LOCUS       DACXMZ010000001        71129 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.001, whole genome shotgun sequence.
ACCESSION   DACXMZ010000001 DACXMZ010000000
VERSION     DACXMZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 71129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.001"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(333..854)
                     /locus_tag="JD016_00005"
     CDS             complement(333..854)
                     /locus_tag="JD016_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAU4998008.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
     gene            1020..1586
                     /locus_tag="JD016_00010"
     CDS             1020..1586
                     /locus_tag="JD016_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAU4998009.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(1803..3866)
                     /locus_tag="JD016_00015"
     CDS             complement(1803..3866)
                     /locus_tag="JD016_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAU4998010.1"
                     /translation="MKAGEMEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNIIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(4247..7582)
                     /locus_tag="JD016_00020"
     CDS             complement(4247..7582)
                     /locus_tag="JD016_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAU4998011.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLYGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(7631..8059)
                     /locus_tag="JD016_00025"
     CDS             complement(7631..8059)
                     /locus_tag="JD016_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998012.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(8049..8429)
                     /locus_tag="JD016_00030"
     CDS             complement(8049..8429)
                     /locus_tag="JD016_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAU4998013.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(8462..8770)
                     /locus_tag="JD016_00035"
     CDS             complement(8462..8770)
                     /locus_tag="JD016_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998014.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(8803..9903)
                     /locus_tag="JD016_00040"
     CDS             complement(8803..9903)
                     /locus_tag="JD016_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAU4998015.1"
                     /translation="MEYIKLNELCYINIGKTPSRNTSDYWGSGNRWLSISDLKEKYIS
                     KSKEEITDLAVEKANMKLVPKNTVVMSFKLSIGRVAILKEDMFTNEAIANFQIKNNEL
                     ITSEYLYYALRTLNFNNTDRAVMGATLNKSKLNDIKIPYFTICIQNKMVEVLNKAQEL
                     INKRKEQIEALDELVKSRFIEMFGNVITNSKDWDTELLGEISNLKAGKNIKAKDIYEK
                     NSHELYPCYGGNGLRGYVKMYSHKGTFNLIGRQGALCGNVKYVNGKFYATEHAVVVQP
                     KVDINSYWLYFTLKELDLNRLSTGAAQPGLTVGKLNEVEIPKVPVYLQNKFVDFVRQI
                     DKLKSRMEDSLKELENNFNSLMQKAFKGELFN"
     gene            complement(9907..11370)
                     /locus_tag="JD016_00045"
     CDS             complement(9907..11370)
                     /locus_tag="JD016_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HAU4998016.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIAVGLEELERMLEV"
     gene            complement(11880..12749)
                     /locus_tag="JD016_00050"
     CDS             complement(11880..12749)
                     /locus_tag="JD016_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAU4998017.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(12769..14685)
                     /locus_tag="JD016_00055"
     CDS             complement(12769..14685)
                     /locus_tag="JD016_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HAU4998018.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGSLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAAIDNGDNLHLKTLSQLSSKLMNEEFLNELLNSETSR
                     EIVSKLNNEEIKSV"
     gene            complement(14688..15608)
                     /locus_tag="JD016_00060"
     CDS             complement(14688..15608)
                     /locus_tag="JD016_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAU4998019.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(15710..16462)
                     /locus_tag="JD016_00065"
     CDS             complement(15710..16462)
                     /locus_tag="JD016_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAU4998020.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(16758..17375)
                     /locus_tag="JD016_00070"
     CDS             complement(16758..17375)
                     /locus_tag="JD016_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998021.1"
                     /translation="MNVIKKFMFVILIINILGCGISLILQSSLGSDAITLLNEGIHIK
                     LGISYTIAGLIYNGTLLLIALLLNKKSLGLGSCVYVLVAGIFIDLYIYLLLDFNISSL
                     GIIIRIMSFIIGHILMCSGFAMLIKFDIGMSPLDAILIYIEGKFKYPYKVLKTIADVI
                     FLILGVYLGGNLGFGTIFSILCTGTTISIVSKMISVNKKRYYSNI"
     gene            complement(17474..18919)
                     /locus_tag="JD016_00075"
     CDS             complement(17474..18919)
                     /locus_tag="JD016_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAU4998022.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEEARYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKKRYKKDSFYWYQNVIRTNGEELKEK"
     gene            complement(18941..19294)
                     /locus_tag="JD016_00080"
     CDS             complement(18941..19294)
                     /locus_tag="JD016_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAU4998023.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(19323..20555)
                     /locus_tag="JD016_00085"
     CDS             complement(19323..20555)
                     /locus_tag="JD016_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAU4998024.1"
                     /translation="MNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMVFVGSL
                     VTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVAGLAGA
                     GLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGEDSALP
                     DFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFVLLNFL
                     VVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWITLGGL
                     GATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPMWIGTV
                     VISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLLFMIWY
                     PFFKAYEKEEIKLESQEV"
     gene            complement(20578..20895)
                     /locus_tag="JD016_00090"
     CDS             complement(20578..20895)
                     /locus_tag="JD016_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU4998025.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            21137..23050
                     /locus_tag="JD016_00095"
     CDS             21137..23050
                     /locus_tag="JD016_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAU4998026.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     NRCPIPFVYVNPYLNRKDYDNIQSKFTQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(23173..24327)
                     /locus_tag="JD016_00100"
     CDS             complement(23173..24327)
                     /locus_tag="JD016_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAU4998027.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLDSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(24606..27221)
                     /gene="mngB"
                     /locus_tag="JD016_00105"
     CDS             complement(24606..27221)
                     /gene="mngB"
                     /locus_tag="JD016_00105"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAU4998028.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEDWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGKKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(27365..29290)
                     /gene="mngA"
                     /locus_tag="JD016_00110"
     CDS             complement(27365..29290)
                     /gene="mngA"
                     /locus_tag="JD016_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAU4998029.1"
                     /translation="MEFKKITNENLVFLKQKIKTKKDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIEDWESVDPNNKVEII
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDSIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(29420..30145)
                     /locus_tag="JD016_00115"
     CDS             complement(29420..30145)
                     /locus_tag="JD016_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAU4998030.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNENKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(30788..30952)
                     /locus_tag="JD016_00120"
                     /pseudo
     CDS             complement(30788..30952)
                     /locus_tag="JD016_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089602.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellobiose-phosphate degrading protein"
     gene            complement(31071..32189)
                     /locus_tag="JD016_00125"
     CDS             complement(31071..32189)
                     /locus_tag="JD016_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAU4998031.1"
                     /translation="MIAVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKKKAGFPKF
                     KSKKINRFSYKTNFTNGNIVYCGQHIKLPKLGMVKVRDKQVPKGRILNATISKEPSGR
                     YYVSLCCTDVDIEAFENTNNHIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRSISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            complement(32191..32289)
                     /locus_tag="JD016_00130"
                     /pseudo
     CDS             complement(32191..32289)
                     /locus_tag="JD016_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            32546..32695
                     /locus_tag="JD016_00135"
                     /pseudo
     CDS             32546..32695
                     /locus_tag="JD016_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589351.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32931..33524)
                     /locus_tag="JD016_00140"
                     /pseudo
     CDS             complement(32931..33524)
                     /locus_tag="JD016_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089602.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
     gene            complement(33526..34953)
                     /locus_tag="JD016_00145"
     CDS             complement(33526..34953)
                     /locus_tag="JD016_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS acetylglucosamine transporter subunit IIB"
                     /protein_id="HAU4998032.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            35344..36066
                     /locus_tag="JD016_00150"
     CDS             35344..36066
                     /locus_tag="JD016_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU4998033.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            complement(36214..37395)
                     /locus_tag="JD016_00155"
     CDS             complement(36214..37395)
                     /locus_tag="JD016_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAU4998034.1"
                     /translation="MKHNFDEVYNRKGTYCTQWDYIQDRFGKKDLIPFSISDTDFTVP
                     KPVYEKLQEILQHQIYGYSRWNHNDFKGSITGYYNRRFEAKIESDWVLYSPSVMYSVA
                     LLLRLLCNNKKKVATFNPMYDAFFSVIEENGFELEKIQLYEKDGKFNIDFDEFEDSVK
                     NCDVLLLCSPHNPTGKVWKEVELEFLINTCKKYNIKIISDEIHSDIIMPDNKHLPIVK
                     YLREYKELYLVSSGSKTFNYPGLIGSYAVIANKNIYDEFLSITRRRDFLNSVSLPGMY
                     ATMVSYNECDYYIEQLVEYIDNNLSYVSQFIKENFEDIKFTKPEGTYLAWIDCRDVPF
                     TCEEIQDSLINIGGVGIMRGEVYGSEKYLRLNCGCPREKLKKGLERFKQSIDYLYFNN
                     K"
     gene            complement(37616..38953)
                     /locus_tag="JD016_00160"
     CDS             complement(37616..38953)
                     /locus_tag="JD016_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAU4998035.1"
                     /translation="MFKKFEQFMNKHLTPIAQRMDKQHHLSAIKKSMVAMTPILIIGS
                     LSLMPEAIGNLTEKAIGANAITSFITNNMDSLQIPFYLTIGMMGFLVCMCITYFLSEH
                     YKLYVPGAMSLAAVGFLLMSFNFTEDGGMDIKYMGTKGLFIAMVAGVLAVELYRWCKK
                     KNFTIRMPESVPDFVSSSFELIPTTVIIVGVFIGIRIITLQVFGVLPPEILTQFLAPL
                     VGSLDNPFTFAFITFLGITLFFFGIHPSVLSPITAPITAQFLAENLEAMAVGQSLPHF
                     YTGGMVSAFANFTGTGVTFGLVFWYLMSKRVDFKRIGQVSLVPALFGINEPILFGGPI
                     VLNPTFFLPFVIGGTILGTFPAFLMNAGILAKPFFNPPYLGVFIEGFLVNGHWLTPIV
                     QAFQMVGSILIWYPFYKMYEKQCDERDTKQVQEIQEESVISPEDLGLLGDLDF"
     gene            complement(38992..39318)
                     /locus_tag="JD016_00165"
     CDS             complement(38992..39318)
                     /locus_tag="JD016_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU4998036.1"
                     /translation="MRIILCCAGGFSTTMLMKNMENVVKNSKKLNDDDFEFKAIPVDL
                     LKNEVDNCDVLVIGPQIAHKLDYIKPILEPKNVPYVIIDQNTYGKMDGATALKMALIA
                     HKKANM"
     gene            complement(39321..39641)
                     /locus_tag="JD016_00170"
     CDS             complement(39321..39641)
                     /locus_tag="JD016_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAU4998037.1"
                     /translation="MENLEMKIMEIIVSSGDSKSKSFEALNKVKEGDYEGARACIEEA
                     RKIDIEAHNAQTALIQSELSGDCESKSMVSLLMVHAQDHYMTSQLSRDLIEKLIDIFE
                     SKEA"
     gene            complement(39659..40687)
                     /locus_tag="JD016_00175"
     CDS             complement(39659..40687)
                     /locus_tag="JD016_00175"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAU4998038.1"
                     /translation="MSIDYLKKLCEADGIASNEQEIRDLLAENINGEISFDNLGSMIV
                     HHRGNGLKMMFCAHMDEVGFMVRHISDIGMLHLINVGGVKDSAKSFQKVNISKVDGTK
                     VKGLINCSYDENNKVKDLYADIGVSTAKEVEDLGINIGDMVCFASESEMLNENVLMAK
                     ALDDRIGCYILKEIDRRVNQIKKNDNDIYLVYTSSEEVGIRGGKTTSKVVNPDIVFAV
                     DVACAPDLIRNYTNQRQLSKGCMIVHYDKTMIPNKKFLNYMKDLATKYDIKFQCDMFS
                     GGGTDAGNAHLANEGKLAIVLGVPLRYCHGNYSMVDMRDVEEVINLCTAVINDMNMEK
                     YNNILKFI"
     gene            complement(40699..42540)
                     /locus_tag="JD016_00180"
     CDS             complement(40699..42540)
                     /locus_tag="JD016_00180"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00359.20,HMM:PF08279.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU4998039.1"
                     /translation="MRILKIINDLENTNKYLSIAYFIDKYSVSKRTLQNDFSYLSKVS
                     SNKGFKIIQKRGLGYLLEIEDNTLFKKFKVELFEYLDQPKLNVESIIACLILNEDYIT
                     IDRILEELKTSRSVVKGYKEDIDSYLKKYNLKLERKSHYGLKIINSMKERRKLIVELY
                     IKENEIIKKYIDSKLENDFKEVQEKFINKLKENNLVINYIELKEILSWLKVTMYIDRQ
                     SKLLELDDSTNEYELNLSSEDYKILKSLIKKKARKKFRRQKGADNLRDDLEDFLKQFD
                     EENNTYFNEDEEFKRSLLNHLTELLERSTFSISYQNPLIDEIQIKYPVAFNIAILLGK
                     IMYKKYNVEIQRDEIGFIATYFAMNMEKQAIYKFRKYNSIAIVCSSGGGSAQLIKFKI
                     SSLFENTNIKTFSLIQMKELEKFNPDIIFSICELDIKLEVPIVYIKELLDDYDILRIK
                     ELIMFEKFKEYSLENKGQAFICKFLRNDYFNIDNDSIDYIKCLEDMSKNIELKGAGNY
                     GYSENVLKREYFATNIYINGVAIPHPIDIDGNENLISVKILQNPIEFKGKKVSLIFMI
                     CLKKENIGLYKKISSSLYEIMNDIETVQLLVNAKTFKEFVALINQRI"
     gene            43047..44714
                     /locus_tag="JD016_00185"
     CDS             43047..44714
                     /locus_tag="JD016_00185"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAU4998040.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(44810..46129)
                     /locus_tag="JD016_00190"
     CDS             complement(44810..46129)
                     /locus_tag="JD016_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAU4998041.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQATEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(46170..46907)
                     /locus_tag="JD016_00195"
     CDS             complement(46170..46907)
                     /locus_tag="JD016_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAU4998042.1"
                     /translation="MAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVMCKA
                     IPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLEAISPKRDSFDMK
                     LLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTHTVQ
                     IKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFVIGI
                     QWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(47160..49169)
                     /locus_tag="JD016_00200"
     CDS             complement(47160..49169)
                     /locus_tag="JD016_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAU4998043.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(49223..49354)
                     /locus_tag="JD016_00205"
     CDS             complement(49223..49354)
                     /locus_tag="JD016_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998044.1"
                     /translation="MAQFLLYRSLGNMIDIATMIFRDIKLIKEYVSILNVQFFVLLK"
     gene            complement(49607..51040)
                     /locus_tag="JD016_00210"
     CDS             complement(49607..51040)
                     /locus_tag="JD016_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAU4998045.1"
                     /translation="MSFKKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDILQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(51056..52480)
                     /gene="ascB"
                     /locus_tag="JD016_00215"
     CDS             complement(51056..52480)
                     /gene="ascB"
                     /locus_tag="JD016_00215"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAU4998046.1"
                     /translation="MALRKDFLWGGATAANQCEGGYKEDGRGLANVDVTPQGKDRFPV
                     LAGKMKMFECDDEHYYPAHEAIDMYHNYKEDIKLFAEMGFKCYRMSIAWTRIFPTGVE
                     DVPNEEGLKFYEDLFDECLKYGIEPVVTICHFDVPMYLVKKYGAWRNRKLVDLYVKYC
                     EVIFTRFKGKVKYWLTFNEINMILHLPFIGAGLYIEEGENEEQIKYQAAHHQLVASAL
                     VTKLAHNINQEFKIGCMLAAGNTYPNTCNPLDVWKSIEKDRENYFFIDVQSRGEYPTY
                     ALKELERKGINIVMEEDDKKILKENTVDFISFSYYSSRLTSADPKVNILTEGNVFASL
                     KNPYLDASEWGWQIDPLGLRITMNSLYNRYQKPLFIVENGLGAVDNPDENGNINDDYR
                     IDYLRAHIKAMIDAVELDGVDLWGYTPWGCIDLVSASTGEMKKRYGFIYVDKDNDGNG
                     TLKRSKKKSFAWYKKVIESNGEEL"
     gene            complement(52567..54483)
                     /locus_tag="JD016_00220"
     CDS             complement(52567..54483)
                     /locus_tag="JD016_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit EIIBCA"
                     /protein_id="HAU4998047.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(54557..55417)
                     /locus_tag="JD016_00225"
     CDS             complement(54557..55417)
                     /locus_tag="JD016_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU4998048.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(55771..56958)
                     /locus_tag="JD016_00230"
     CDS             complement(55771..56958)
                     /locus_tag="JD016_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAU4998049.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFDNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKF
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSGEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(56972..58378)
                     /locus_tag="JD016_00235"
     CDS             complement(56972..58378)
                     /locus_tag="JD016_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAU4998050.1"
                     /translation="MGRLKMSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFER
                     VDIDGRSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKT
                     GAIEAVIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGY
                     DSLTAVATVFIGATAGFGAATTNPFSVGIAQALSQIAPGSGIEFRVVMFIIYMAISIG
                     FVMMYANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIY
                     GVLKLGWYITEIAMIFTAIGIISGIASGLKQDDIVNSFISGAGDLMTAGLCIAFARGI
                     VIIAENGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVL
                     APLGDLVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMV
                     VLTILVMAFLTIGLYLGF"
     gene            complement(58958..60610)
                     /locus_tag="JD016_00240"
     CDS             complement(58958..60610)
                     /locus_tag="JD016_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAU4998051.1"
                     /translation="MKDKLQVLSGAMMIPVILLVVAGVFIGLGSAFANMENVSALGLS
                     GLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKKEKGWGALGGIVLFIGM
                     HTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGSFLGIFSYDCSIFGAII
                     AGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLGTAMYFIWPPVGLALSK
                     LGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVGGTMEVGGAIYEGVNNI
                     RLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLADNANKKKAISILIPAIV
                     TAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFNVSIMGESFRNMFPNLL
                     QPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSEEVKLYSKKEYRERQSV
                     DAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNCATRLRVELNSIDGFYE
                     DGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD"
     gene            complement(60630..62033)
                     /locus_tag="JD016_00245"
     CDS             complement(60630..62033)
                     /locus_tag="JD016_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAU4998052.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEVELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(62298..62789)
                     /locus_tag="JD016_00250"
     CDS             complement(62298..62789)
                     /locus_tag="JD016_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAU4998053.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEADLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(62815..63627)
                     /locus_tag="JD016_00255"
     CDS             complement(62815..63627)
                     /locus_tag="JD016_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAU4998054.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(63640..64791)
                     /locus_tag="JD016_00260"
     CDS             complement(63640..64791)
                     /locus_tag="JD016_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAU4998055.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            65061..65702
                     /locus_tag="JD016_00265"
     CDS             65061..65702
                     /locus_tag="JD016_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAU4998056.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            65728..67272
                     /locus_tag="JD016_00270"
     CDS             65728..67272
                     /locus_tag="JD016_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAU4998057.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(67472..68098)
                     /locus_tag="JD016_00275"
     CDS             complement(67472..68098)
                     /locus_tag="JD016_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAU4998058.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(68233..68949)
                     /locus_tag="JD016_00280"
     CDS             complement(68233..68949)
                     /locus_tag="JD016_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HAU4998059.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            69375..70589
                     /locus_tag="JD016_00285"
     CDS             69375..70589
                     /locus_tag="JD016_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAU4998060.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
BASE COUNT        23039 a        12656 c         7643 g        27791 t
ORIGIN      
        1 ataaataaaa ctaaaaaaga tgttgtctca aaatatagat aaatttattt ttcattgcta
       61 aaatgattaa aatatatttg atttaatata cttgatttaa tataaaaaaa gagtgtctca
      121 taaactaaaa agttcacttt tagacactcc tttatatata ttatctactt cttaattcat
      181 ttattacctc tctacatttt ctttcaacta gtggatacat ctttataagt actaattact
      241 ccacttaaaa atgattcagg tactgtccat cagtcatatt tttctgccaa atataatggt
      301 gtttcataat gagacaataa atatttatat ctctacttat cttctctaag ctcgtcaaat
      361 ccaactatag gtatatttaa attatattct ttaagtagct tttctagctc ttctataaaa
      421 atacctttcc catccaccat ttgtatatca tttattttat ccatcaattc attgcaacca
      481 cttaactcac caaaccaacg tttagaacta catgtcttat tataaccaca acttggactg
      541 ccatctattg caatgatact ttttatatca taatcatttt ttaaatattc atcaaactgc
      601 cttacatgag gtattaacat ttgcctacat tgttctctaa aaaacaaatt gtcaaactgt
      661 tcttttacat gtccccacct atttattcca tacataatca tttctgggca tggtagttga
      721 attattccat accctttatt tattaaattt atcgctatat tttgtgctcc cttaaattct
      781 gacaatccct ccactttaga attacaattt aatatacaat gacatactac tacaatctct
      841 ctatttcttt tcataataat ctccttagtt attcttaatt ttaatgatac tacatatttt
      901 gataattata ttatttattt gttctatatt tccaatattc tatatagaaa tatacaatta
      961 gtaatctttt ataatctata ctatgataaa tgtatatatt tttttaggag gtattataat
     1021 tggatactaa aaaagtcaca ttttcttcaa tgtgcatagt tattaatatt gtcttaggta
     1081 tggttatatc tatgataaat ataccattat tatttttaga tacagttggt actgtacttg
     1141 gggctgtcgt attaggtcct ttttggggtg ctcttattgg tggatgtaca aacttagttt
     1201 taggtgttat atcaggtcct acaaatatac cttttgccct tgtaaatatt gttcttggcc
     1261 ttattgttgg atatatttct aagaaaaaag gttttggtta taaagaagct attattacag
     1321 gagtaatttt atctattgtt tgtcctttaa ttggtactcc tatttcagtt ttattgtttg
     1381 gtggtttaag tggaagtggt gctgatttat tggttggttt tttagttcag tctggtcaga
     1441 agatatttac tgctgcgttc tttccaagga ttcttagtaa tatagtagac aaacctttgt
     1501 cttgtattat ggtggtatat tttataagta aaatgcctaa aggttttata aaacaatata
     1561 gtaaaaatac ttctagttca tattaataaa tcatttatta tgtaaggttt attactcttt
     1621 aaaatgctat atactcattt ttatataaca tttttattaa taatttaatt agatagtatt
     1681 tgtattactt taattaaacg taacctcttg atataaattt gagctataaa tctatatcaa
     1741 gaggtgctat tttattttta atataattaa tattctatta cgcaaaaaat gcatatattt
     1801 cattatttta tggaaaatat tacttcattt aactctatat caaactcatt aagtaaaaat
     1861 ctaattatat ttaatttagc ctctgtattt aaatttgttt ctaaatatat attttctgat
     1921 attttaatac cttttcttaa tttatatgca tctctagact taatattatc tataaaatct
     1981 cttttataag ttttgtctac aataatgtta aaccttacag aatccaaatc atataaagta
     2041 agagcaactt gttcataaaa ttctgtccaa tttttagcct tatatgactc tcctaatata
     2101 ctaaatgata ctatactttc atttgtaaaa ttaacatcat catcctctaa cgtaaatata
     2161 ttttcactgt tacttggagg ttcatatgta acacttggat atttccaaat ttctacagct
     2221 ctatctagta atatcttagt tctatttttt atttcttctt cattccattt atctatagat
     2281 gaaatatatt tatttagata aagcctacta tctttaaatc catcttccat ttctttcttt
     2341 tcttcaaatg ttttattact tagttttgaa ttatatcctg tcaaagttaa atttccaatt
     2401 gtatgtaaat atttatcatg tatctcctta tagttatctc ctagtgattc cttccattta
     2461 tttgttaatg tttgtggcat tatatgctct atatttagca tattattatt gattaaattt
     2521 tctaaatcaa caatttcttt attattataa ttctctaacc tctcaagtat atataactta
     2581 ttctttgact tagtattata tatatcttta gtttcaaaag catttgaaaa ttcactgtca
     2641 gtaggaaatc tttgagatga tttcttattt ataaaaatat atcttaatat attagaatag
     2701 ttttctttat aatcactaaa ttttttaatt tcccttccta agttcataaa tactttattc
     2761 agtgcattcg gaggtacatc acatactatt cttcttaata taaaacattc tactaaatta
     2821 agggattcta caagtacttt tttatttatt ataccttcat tataatcatc aaataattct
     2881 aatataaacg gatatgatac aaatatttca agtttgttta tccttttcaa aactttgttt
     2941 acatcattat catcagtaca tgaataagat atctctctat aatagttaga aaatttaagt
     3001 aaatctttta ataaaatttc tatatctata ccattatctg atacatacct tttaaaacta
     3061 aaatatactt tatttttatt cggtatactt ctctccttta tagtcaaata gtctcttata
     3121 aaactactta cgttgctact tgtatttttt tcaattttat accaataatt attataaaat
     3181 tcctcttgta acttagagct tctacccatt aacacaaagt ttcttactct atcagcttca
     3241 cttaaatcta agccagttga atttagactt tcaaagatta attgagggtc atcttcacca
     3301 ttttttaact ctatttctac tatagttaat ttctgtatag catcaaacaa atcatctatt
     3361 gatatattat cttcttttat tctattataa aaatataaat aatttgaaac tatattagaa
     3421 tttctatctg tattcatttc ttcttcaaat agttcatagt acgcttttct atcttctgct
     3481 attggtttta actttatttt tccatcttta tctcttgagt gcttgtttat aagataacac
     3541 tccttaatct gttcttctgt tgtaataatt ccttttgatt ccccaaactc ttttattatt
     3601 tgacacatgg caatcaataa tattgacaca gtagtcactc tttgttgtcc atctattatt
     3661 aaatattctt ttcccatact atatatagat acaactgacc ctaagaaatg agttctaaat
     3721 ccagaatcta ttatatcctc taaatcatca aacaatcttt tacattgttc tttcttccag
     3781 ttataatttc tttgatatac aggtactaca aaacttttgt ttgacccctc taaataacta
     3841 agaaattcca tttctcctgc tttcatatta actcccccta taagttaaat aaattccatt
     3901 cataactcca tgtatctata tattatatca aaattttgac tcataaatgt atatgaaagc
     3961 cttattatat acctttttat acctgttttt attttattaa agtaaataca tgattattct
     4021 ataaaatata caaatactta attttaaaaa ttattattta atcttcttca tgagtaattc
     4081 atagttattt atcaattaaa aagagatatt gcataattat tcaatacctc tctttgtata
     4141 tttacattat ccatatctag ttatctattt caacccaata ttagactacc tactaataaa
     4201 tttattctct acatcattaa caataatttt gcactaaata acatcattag cctatctctg
     4261 atgcattttc tttaatttta tttatagttt taatcaattt atttctctct tcaatatgat
     4321 tttcaaataa atcaattata cttccaacag ttctaaatgg gtcttccatc aacactttgt
     4381 tatcctctat aaatccattc ttgactacat aatcgactat aagtttaaca aaatgtattt
     4441 gcttagtatt aaagctctta ttatttaaaa actctgaaaa agcttcattt gcagcttctc
     4501 tatccaatcc cactagtctt ctcactaatt ggcttactgg agtatctcca aactccttct
     4561 tataatcatc acaattacct aactgtttta agagtatttc ctccaacgtt tcaacatctt
     4621 gctttgttat ctttttatta tttcttaatt taaatattgc taattcatct ttatggctat
     4681 ttaaataata ctctaccttc tttttataat tcttaagatt gtttgcatta tatacagaat
     4741 catttctatc ttctgctaca atcatatctt caaaacttgt ataatatatc ttttgactag
     4801 acttttcaag atacttaata agctctctta atgatatacg tacttcttct acctcaaata
     4861 tatctgaatc ttcccaaaaa tcctctgtca ttgctttttc aatagtatat ttttgttgct
     4921 gaatctgtgg tatagtccca agttttgaaa gtttttctgc tgtagccatt acatgcctta
     4981 tacctttact tgcattatta ttttgtaagt atgcaagttg tatattatac ataactacat
     5041 caaatctttt agccaattca tcatcactat atgaagttat gagtggtgat atatattctc
     5101 ttatctcatt ttgtgctact gctccaatat tgctccaaac attttcattt ttatacttat
     5161 gtacataact taaattcatc ctaactatat aactatcctc atttaaagct tttacagaag
     5221 ttataagttc ttctaacaat tcttttctaa gttctgcata ctcaccttct atatatttaa
     5281 tatcttgaag ttctttaacc atacttacct ttatattaaa taatttttca gtaagtgttt
     5341 ctgctttatt tccctcaaat cctttaggat taaatttaaa aaattcaaaa ttattacaaa
     5401 aatcaaatat taagaatctc tccttatcca tatcaacacc taataaatct ggacataatc
     5461 tagttcctct acctatcatt tgccaaaact tagtcttact tctcactttc ttgaaaaaaa
     5521 caagatttag tatttcaggt atgtcaattc ctgtatcaag catgtctact gaaactgcta
     5581 tttgaggtaa tttattctta tcagaaaaat catctattat agaatctaca taatttatac
     5641 tatagtcaac tgcttttaca aagttcccac catactttgg atacagctta ttaaatcttt
     5701 caactattgc cctagcatgc tttgtattct ttgcaaaaat tatagtctta ccaatctttt
     5761 cttctccttc tattttcaac ccctctgtca ttagcttatt taaaactaaa tctattgtgt
     5821 cagaattaaa tagccactca tttactgcat tattccctat ttcgtctcca atactctcat
     5881 catcattaaa tgtattttca tattcttcct tctcttcatc agaaagctca tcatacttaa
     5941 ttccatcttc cataatctta gtcttaaact caatactagt ataatctact aaatatccat
     6001 cctcgactgc cttattatac tcataagcat atgtaggtac tccattttcc atgtcaaaaa
     6061 ctgaataagt atttttatct atctcatcct ttggtgtagc agtaagaccc attaagtaag
     6121 aatcaaaata atcaaagatt gacttatatt ttttataaat acttctatgc gattcatcta
     6181 ttataattaa atcaaaatgg ccaggtgtaa acaatctctt tccatcctta gacttcgtat
     6241 catcaattgc attcatcatt gttggatatg tagaaaatat catcctagca tcttctgggc
     6301 tgtctttgct atccaataaa ttacataacg ccaagttagg taatagctta gtaaaacttt
     6361 ttttagcttg ctttaccaat gcttttctat ctgctagaaa taatatattt ttaatccaat
     6421 tatgtcttga aagtacatca actatagata tagcaattct tgtttttcct gtaccagtag
     6481 cacataccaa aagagttttg cgttgcttat tttcaagagc ctcacataca gccctaatag
     6541 cttctttttg ataatatcta ttggatattt catcatttat tgttacagaa cttaaagact
     6601 tcttactctt tcttctatct atcataagtt gtaattctga ttttttataa aatccataaa
     6661 cttttctatc tgaatagtca tcccaaatat acatatcaaa accattagtc aaaaatatta
     6721 ctggtctttg actatatctc ttttccaaac aatctgcata taatttagct tgttgttgac
     6781 caatttttgg atctttactc gttcttttcg cctcaactac tgctaatggc ttaccatttt
     6841 caccataaag aacataatct acatatccta caccactttc attaggcata ccttgtactt
     6901 ctatttcctc ccctatatct tttccaaatt cccaacctgc aagctttaat tctatattta
     6961 tatatctttt tcttgtctca aattcgctta cttcgtctat cttgaaatca tagtttacag
     7021 tattaacttt tctcttttca gttagtgact ttcttaattc ctcattttct tttattactt
     7081 cttcaagctt tttatctttt gaacttaatc tctcataaag atttttaagt tcgtccggtc
     7141 tcgtcctttt ctcttcccca taaagtaata aatcttcatt aaagtcttct gctgtatatt
     7201 catctgaata acaatagtct atccaactaa caaattgatg caaattgtta agtgaaagta
     7261 ttgcttctcc tctctctatg ttcgcactag tatgtacaga taaattacct aatttaacta
     7321 tatattttaa taaaggtaac atatcatagt ctataagttc tataaaacta ttattatgaa
     7381 tcaacgtact gagattatct tgataaggca atactaaatc actatcatta gcatatagcc
     7441 attttactgc aagctctaaa gctcttcttg ttatagttgc acaagtagat ggactaacta
     7501 gtatactttt ttctgcttct aagcaagcag tattaaaact attgaaattt ttcttctttt
     7561 gtaaaaaatc aaaattaccc aagcttttat cccccttttg tcccttagtt aaaataaatc
     7621 tctctaaaaa ctatttctta cagctgtttc ttatactaac aaatacttct tcattttctg
     7681 taaacaattc gtacatatct tcaaactgtg gtatatcata tccatgagaa tcatcatttc
     7741 tcaattttct aagtgtttct ataactatag aagaattttt aggtaataaa ttgtgatact
     7801 cacaagctaa aaggtagtca taaaagtttt tagattttat gtttaaatca attgatttat
     7861 aatacaacat tgcttttgtt aattctagaa atgataccat atagctttta aagccttctt
     7921 ttatgtatct agcccataac tcaggcattt cttccttatc atataagatt ttgaagtttt
     7981 gcaggtcata caagcaagaa ccaaaatccc tatttacttc ttctattgag ttccaagata
     8041 atttgtttct aattaccata catgtccctc caatctaaga tatctattac atcactgttt
     8101 tcaaaataga atttatttat ataatcctta aaatctttag attcataaat aacttcacct
     8161 tctaaaatag cttttttaag tgctattgta taattatctg taacaatttt taggtctaca
     8221 tcaatgtcta atatatttct tagagcttct gtcttagttg caagttctgt aattggtact
     8281 tttttgatgg gaatccaagc tatgtctata tcactttctg atgtatgttt atctgttcca
     8341 taactaccaa atattatgat tgctagagta ttttcttttg gaaatacatt tattattgag
     8401 tttttaatct catttggtat atttctcatt taatcacctc ttgtttatta ggtaatattg
     8461 cttaattaaa tttatatcca taatctttta atctatttat acatatttct ctttcttcat
     8521 cagtaaacaa ctctttatac ttttctttta atactaatac ttctaatgat aaatctaatc
     8581 tcttacactc ccatagcctt gtaaatcctt cagtagctcc ttctttattg attaattctt
     8641 tagcagctaa taaggctcct ttgttattta tcagttgtaa aaatcttgtt ggcttatagt
     8701 tacattgtaa atcagcttct ttatagatat ttaccatgtc atggtataaa tctagctcta
     8761 tttgattcat agattttatt cccccatttt aatacttgta ttttagttga ataactctcc
     8821 tttaaaagct ttttgcatta atgagttaaa gttgttttct agttctttta agctatcttc
     8881 cattcttgat ttcaatttgt ctatttgtct gacaaagtcc acaaacttat tttgaagata
     8941 tacaggtact ttaggtattt caacttcatt taatttgcct actgtaagac ctggttgtgc
     9001 tgctccagtt gataatcgat ttaaatctaa ctctttaagt gtaaaatata gccagtaact
     9061 atttatatca actttaggtt gtactacaac tgcatgttct gtagcataaa actttccatt
     9121 tacatatttt acattaccac ataatgctcc ttgcctacct atgagattaa atgttccctt
     9181 atgtgaatac atttttacat atccccttaa accatttcct ccataacaag gatataattc
     9241 atgtgaattt ttttcataaa tatcttttgc ttttatattt tttccagctt ttaagtttga
     9301 tatctctcct aataactctg tatcccaatc tttactatta gtaatcacat ttccaaacat
     9361 ctcaataaat cttgatttaa ctaattcatc caatgcttct atttgctctt ttcttttatt
     9421 tattaactct tgagctttat ttaaaacttc taccatttta ttttgtatac aaattgtgaa
     9481 atatggtatt tttatatcat ttaacttgct tttatttaaa gttgcaccca taactgctct
     9541 atcagtatta ttaaaattta aagttctcaa agcataatat aaatattcag atgttataag
     9601 ttcattattt tttatttgaa agttagcaat tgcttcattt gtaaacatgt cttcttttaa
     9661 tatggcaact ctacctatag acaacttaaa actcattaca acggtatttt taggtactaa
     9721 tttcatatta gctttttcaa cagctaaatc tgttatctct tcttttgatt ttgatatata
     9781 tttttctttc aaatcagaaa ttgataacca tctattacca cttccccaat aatcagaagt
     9841 atttctagat ggtgttttgc caatatttat atagcacaat tcatttaatt ttatatactc
     9901 cataccctat acctctaaca ttctttctaa ttcttctaat ccaacagcta tttccttctc
     9961 taactcccta actctaccta taatcacact aggagcatca taaactactt cctcatacac
    10021 tacctcttta tacttattaa tagataaatc atacccattc tctcttatct cttccacatc
    10081 aacaaaaaag ctttgttctg ttctttttct atctacttcc ccatcaagat tatcaaatct
    10141 cttaataata tcctctatat cactatcctt tatctcattt ctcttatcat ccaaagaata
    10201 accatcagct ttcatatcat agaaccatac cttatcagtt ccacctgtcc ctgttttagt
    10261 aaatatcatt atagctgtag atactcctgc ataaggctta aacactccac ttggcattga
    10321 tattatagct tccaatttat gattatcaac tatttctttt cttatcgatt tatgtccacc
    10381 tgttgaacca aacaatactc catctggcac tatacatgcg catcttccac caatttttaa
    10441 aactcttaaa aaaagtgcca agaataataa ctctgttttc tttgtcttag tcacttttaa
    10501 taaatctgct gacactgctt cataatctaa acttccctta aaaggaggat ttgctaacac
    10561 caatgtaaac ttatctttat ctgtattaac ctccgataaa gaatccttat actctatatt
    10621 aggattgtca acgccatgca acatcatatt catagcacct attctaagca tagttctatc
    10681 catatcaaat ccatagaaca taccattatt aaaatgctcc ttcagccctt gcactaagaa
    10741 aagacttgaa tgattatccc ttaagtattg ttgtgatgat actaaaaaac ctgctgaacc
    10801 tgctgccggg tctactataa tatcttctgg tgttggttta ataagtctta ctatcatatc
    10861 tattatgtgt cttggagtcc taaattgacc attagtacct gctgtagcca ctttggataa
    10921 taaatactca tacaaatctc cctttgtatc tctatcctta cttaattcta aattactaat
    10981 accatcaact attttagata acataagagg cgtaggaatt ttaaatattg catctcccat
    11041 gtactttgca taagctgagt ctttatttcc atgtaatttt tttatgaaag gaaacacttc
    11101 attttgtact atttcataca ttttttctga atttccttca ttagaaaact tactccatct
    11161 taaatgttga tgttcatcat caaatatcct ttcaaaagaa actcccagaa gagttgcttc
    11221 tgcttcattt tttgtttcta cttcatccaa tcctttaata aataaaagat atgtaaattg
    11281 ttctataact tctaatggat tagttaatcc accagtccaa aatgtctccc aaatcttgtc
    11341 tactttgctc cttaattctc ctgtaatcat atatgacttt ccctccagtg ttcataaaat
    11401 aatttaaata tatattttag tcgtacgcct taatagttag tgtcttatgt taaatccaag
    11461 tttttactac acagttatta aattattttt cctatttaca tgttttagtt aaaaaactaa
    11521 tgcaatactc ttattttatg attattatat ataccataca aaattatttt atcatatatc
    11581 catcatagaa gtcatctacc tggataactc ttaccatatt ataacttttt aatattaatt
    11641 taaacttaga aagaaatttt ttatttaatt atatcatctt ccttatttca aatttctcaa
    11701 ttacaatctt aatcaaaaaa taaaataaga tgctgtctca aaatagatat aatcttcttt
    11761 tttattttcg gaatgatgaa aatatattta atttcataca aaaaagagtg cctcctgaac
    11821 taaaaagttc attttgagac accccttaat ttaatcttac ttattctctc cctttattat
    11881 taacacttca ctatctctat tgcttttcct acacatccac aaacttccat cctactctta
    11941 acaaaatctt taactgactc taaacctttt ttaccatatt tcttagggtc aattacctct
    12001 gggtcagaat taagcacatc cttaacccca ttgctataag caatccttaa ttctgttgca
    12061 taattaacct tgcaaatccc tctttttata gattccttaa taacttcatc tggtattcca
    12121 gacccaccat gtaatactaa tggaactgat acaacatctc taatttcaga taatctatct
    12181 aaatcaagct ttggctctcc cttatataat ccatgtgccg tacctattgc tactgctaaa
    12241 gatgataccc ctgttctctc tacaaactct tttgcttcta atgggtctgt atatgctccc
    12301 ccatcaccac catctaaatc gtcttcctta cctccaactt tacctaattc agcttcaaca
    12361 ggtatatctg aaggcataca tgcatctaca acagatttag atattgaaat attatcttca
    12421 taactttcat gagaaccatc aatcataata gaagtatatc caactctaag tgcctgcata
    12481 gctagtccaa agcttgaacc atggtctaag tgcattgcca ctggaacact tgccttctct
    12541 gatgctacct ttacattcgc taaataataa tctagtcctg catactttat tgttgaaggt
    12601 gttgtttgca atattactgg tgatttaagt tcttcagcag cctctattac tgccataacc
    12661 atctccatat tttcaacatt aaatgcacct actgcataat gcccttcttg tgcatctaat
    12721 aatatttgtt tagttgtcac taatgccata atttctcctc ctatatgttt atactgattt
    12781 aatttcttcg ttatttaatt ttgatactat ttctctactt gtttcgctat ttaataactc
    12841 atttaaaaat tcttcattca ttaattttga agatagttga gataatgttt ttaagtgtaa
    12901 attatcacca ttatctatag ctgctatcat aaatataata tgtgccaagc ttccatcttc
    12961 tgattcaaag tcaaaccctt cttttgatac acctactgct acacgtggta cctttactgc
    13021 atctgttttt gcatgtggta tcgctatccc cataccaaaa cctgtagatg atatttcttc
    13081 tcttttaagg atttcttctt taaacttctt tttatctaaa agaacaccag atttatcaag
    13141 tattccaatc atttcatcaa ttacatctgt tttatttttt cccttaacat ctaaaattac
    13201 tgtttcttcg ctatataaag aaatattatt agatacgttt tccgattctg cactttcatt
    13261 tttatgttca tcttttgttg catttgtatt ttctatagta tttttctcat ttactacttt
    13321 aggtgtaacc atcatataaa gtacagcacc tactaatgaa cctacaaata tacaccctac
    13381 ccataaaagt ggcttattga ctaatcctag aaccaaaaac cctccatgtg gagctggtac
    13441 ttctattttc attatataag ttaaaattga tgatattgat gatgcaatca taagtactgg
    13501 caacactttt aatggatttt ttgctgcaaa aggtattgcc ccctctgtta tatgagtact
    13561 acctagtata taattgatta atcctgctgc tctatcttct tctgtaaatc tatttttaaa
    13621 taaagtagat gctattgcta ttaccaatgg tggtgttata caagctgcag atacacctgc
    13681 cataaataaa taatttccct gtcctagaag catagttcca gttacatatg ctgctttatt
    13741 tactggacct cccatatcaa atgctgacat acatcctatt actattccca aaattatagg
    13801 gcttgaattt tgtaaccccg atagaaagtt catcatagca ttgttaagtg ctgaacatgg
    13861 cccaccaagt aacaacattg ctacacccac aagacctacg ctgacaatag gaattataaa
    13921 aatcgattta agaccttcta attgtcgtgg taattttctt aacaaatgta caaatagttt
    13981 aactaagtaa cctgctaaaa atcctgatat gattccacct aaaaaccccg aacctgtatc
    14041 aaatgatatc attcctccaa caagtcctgc taacatacca gctcttcccg atatagattg
    14101 agctataaat gctgccaata caggaaccat caagttcata gaatatccac caacagattt
    14161 taatgtagct gctatttgat tatattgttc tgaatttggg tcaaatgaat aaatccccca
    14221 taaaaatgat atagcagtaa gtactccacc tgctactact aaaggtaaca tgtgagatac
    14281 accattcatt aagtctttat acaacttttg accaaacgat aaacttcctt cttctgcaat
    14341 tttactgctt gataagtttc cttttctaat agctactttt ccatcaatac atctttgtat
    14401 aagttcatta gctttatgta taccttcttt gacaggtact tctattactg gctttccatt
    14461 gaatctgtcc atatcaacat ttttatcaca agcaactatt attgcatctg ccatttcgat
    14521 atcttttgct gttaacttat tttctacacc tatagctcca ttcgtttcaa cttttatgtc
    14581 acaccctaat tccctggctg aattttttaa tgcttcttct gccataaaag tatgagctat
    14641 tccagttgga caacctgtta cccctagaat cattttcttt cccattttta taccaccttt
    14701 ctgactctta tattttttat ataatcttcc acattctcca aattacctat accattactt
    14761 tcagccacac ttgctcctgt tgctgctgac ctttttaatg cttcttcaat atgttctgga
    14821 ttttccaacc atatacttaa aaaagcagct aatgcagaat caccagcaca tgcagagctg
    14881 actactgtaa ctggttgagc actagcataa taaattgatt ttccattata aaaataagaa
    14941 cctttttctc ctaaagttag caatatattt tgagcacctt ttaaatgtag taatttaagt
    15001 acatccttaa tatcttcttc atctcttaca ataattccaa atatatcttt gatttcttca
    15061 tcatttggtt ttatcaaata tggattatac tgtagtaact cctttaattt tggtgaactt
    15121 atatcgagta ttgtctttat acttttattt ttacaaattt taaatatatc ctcatagtaa
    15181 ttatcatcaa taccaggagg tagacttcca cttattgata agtaacttat atctgctaat
    15241 gactgtatct tatctagcat atctagtttt tgtttttcat ttacatatga cccagagttt
    15301 acaaatttaa attcatcact tccatcattt aaaaatatat ttatacgagt tgtatcttct
    15361 acccaagtag gcaatacgtc aaatcctctc ttttcgcttt cttcaactat atattttcct
    15421 gaaaatcctc caaaaaatcc taatatctta gattctatac cataatgacc tagtacaaaa
    15481 gttacattta atccctttcc attaggagaa tatacagcat ttgatgtacg atttactaat
    15541 tttctcttta tcccattagt agatatattc atatctattg caggatttgt agttaatgta
    15601 tatatcattg tcttccccct tgcttgtgct gtgtatgttt tcattataat aaaaaagtct
    15661 atgaaaatct tttcacaaat agaaaatcga ttttcataga cttttcaatt taattttcag
    15721 aattattata tctgaccata taatctataa ttatcctaga tataatatac aagggcaatc
    15781 tggaagatac ttcatattca gaaattgata tatgttcatg tttgtatcct acaatatata
    15841 aatccgaata ctttataagt tttgcacttt cactacaaca gcaagttata accttagctc
    15901 caactaaatt ggcattctgt gctgattcta caagctctat ggtatttcca ttcaatgaaa
    15961 atataattat aagttcgtct ttttttattg atttagattt tattttcatt atgttggggt
    16021 catttataaa aacaacatta aaatcaagta attgcaattt aagactaaac tcttttgcta
    16081 catattcagt aagccctctt gctaaaatat atatcttact agatttattt atagttttta
    16141 ttatattgat tagtgtagtt aaagatattt gttctataac tctctgtgct tctataaagg
    16201 atttatttat tatatctccc atattttgta tatcatcatt ttcatctttt actgttaatc
    16261 tatacctaag ttcattgaat ccatttaaac cgcattttct aattgctctt gaaacggttg
    16321 ctggagatga atatgtttca tgagcaatct ctactataga taattctggt aatattgatt
    16381 cattttcatt tataaattta attatttcaa gttccgtctt cgttagattt tcagaattac
    16441 ttttgtttag ctcaattatc atatgttatc tccccttata taagttttat ttgtctaaat
    16501 ttatcgagta atataatata ttacctctta actacttatt tttatataca attaagattt
    16561 agtcaatgtc tatatatgta aatttataat ataataattt tatgcttcaa ttaattatgt
    16621 aattattaga gtattttgta ataaaaattg gagttactca attactgaaa ctgagtaact
    16681 ccaattttta cgacctcaaa ttaactcgaa ttattgaaaa tgattcataa acattatcca
    16741 tactctcact tatcaattta tatatttgag taataccttt ttttattaac agaaatcatc
    16801 ttactaacaa ttgaaattgt agttccagta cataatattg aaaatatagt tccaaaccct
    16861 aaatttccgc ctaaatatac acctaaaatt aaaaatatta catctgctat agtttttaaa
    16921 actttatatg gatacttaaa tttgccctca atatagatta atatagcatc taaagggctc
    16981 attccaatgt caaattttat aagcattgca aatccactac acataagtat atgtccaatt
    17041 ataaagctca ttattctaat gataatccct aaactactta tattaaaatc taataataag
    17101 tatatataca aatctataaa tattcctgct actagtacat aaacacagct tcctaatccc
    17161 aaactttttt tattcaatag caatgctata agtaatagag ttccattata tattagccct
    17221 gctattgtat aacttatacc taactttata tgtataccct catttaaaag tgtaattgca
    17281 tcactaccaa ggcttgactg gagtataaga gatataccac atcccagtat atttataatt
    17341 aaaattacaa acataaattt ttttatgaca ttcataatta ataaaccacc ttatttataa
    17401 ttataatccc tcttataaaa ctgtattaat aacatagtat ttataagagg gattatttat
    17461 atttaaaaac tatttacttt tcttttaatt cttctccatt agttcttatt acattttgat
    17521 accaataaaa actatctttt ttataacgtt ttttacttcc atttccattg ttatctatat
    17581 ctacatatat aaatccatat cttttttcca tttcttgaga tgaacaactt actatatcta
    17641 ttggtgacca catacaataa gctaatattt cggttccatc agctatagct tcacctattt
    17701 gttctatatg ttttcctaaa taatctattc tgtaactatc atgaactttt ccatcttcaa
    17761 atttatcatg cattccaaat ccattttcag ctatcattat tggcttttgg tatctatcat
    17821 aatattgaga taaagtattg tataatcctt ttgggtcaat tgcccacccc caaggatttg
    17881 cttttaaata tggattcttt atattgtcag ttggattcat ggtgttttta cttgctgata
    17941 ctgttgtaga gtaatagtat gatagagcta gaaaatccat agtattttct tttaagattt
    18001 tatcatcacc ttcctccata actatattta tgttattttc tttgaaaaat ctcttaatat
    18061 agactggata ctctccccta aaacttacat cagtgaaaaa atattgtagc ctatttcttt
    18121 ttaaagcaaa tactacattt tcagggtcac aatcttctgg ataagatgta caatctgcca
    18181 tcatcatacc tatcatcata tctgaattta tttcttttcc aagcttctta gtcaaagctg
    18241 tagctatgaa ttgatgatga atagcttgat atctagcctc ttctacatta tctacttggt
    18301 ctacacaaaa tgcaactgaa ttaaatgatt cataatgtat taaatttatt tgatttataa
    18361 ctatccaata tttaacttta tttttatatc tatttagtat taattctgca tatctaacaa
    18421 agaaatctac cacttttcta ttagaccatc ctttatattt taatactaaa ttcaaaggag
    18481 tctcatagtg aagtagtgtt acaataggtt ccataccatt atctattact tcgtttataa
    18541 gattatcata aaactttaat ccctcttcat taggctctaa atcatctcca tttggaaata
    18601 ttctagccca atttattgaa aatctaaaac actttaaccc tagctctttc aaaagcttta
    18661 aatcttcttt gtatgtatga taaaagtcta ttccactacg cttaggataa tatccattat
    18721 tatctttcat agcaaacttt attctttcta tagtcatatc agactcataa ggtttagata
    18781 tatctctatc tttatcatac atatgtacat cagctaagca ccaacctttg ccaccttcat
    18841 tccatgcacc ttctaattga ttagctgcta cagctcctcc ccataaaaaa cctttaggaa
    18901 acttacccaa tttaatcact ccttatttta tttatttaaa tcatttacta ttatttacct
    18961 tatattctat agactttact attcctacta aagacatagt tatatttaac tcgctgaata
    19021 tggtcataag agtatcttga gcatgtgcaa ataacagcga atgtgctctt ttctctccac
    19081 cagcttcccc ttgtataatc ttggtttgag ctctatgtgc ttctgtcata aacccttttg
    19141 ctttagctag ttttaaatca gcatttctat aatcaaactt ttccaaatca ttaagagcat
    19201 gtttaatttc tgttctagcc tcacctgcac gaagtattat ttgcattgcc acctctacta
    19261 attcttcatc tattatatgt tcttcaattg tcatatttat aatttccttt caatttctaa
    19321 atttaaacct cttggctctc taattttatc tcttctttct cataagcttt aaaaaatgga
    19381 taccatatca taaatagcaa tactatattt attgctaaaa caataagacc tctaacatct
    19441 tgatttacca taaaagtaga tataccaaaa ggaacatacc ataaattaaa caccttactt
    19501 ggaattgtca caaatcccat actcattgat aagtaagtag ttattgatat tactactgtt
    19561 cctatccaca taggtaacat taaaaatggg ttccatgcca ctggagctcc aaatattact
    19621 ggttcattta tattcattat agaagggaca atacatgctc ttcctattgc ttttattctt
    19681 ccaactctgg ataaacacat cattataact aatggtaatg tagcacccaa ccctcctaaa
    19741 gttatccaac ctgtttgaat aacttcgtaa acatatatat tagttgctac tcctcctgat
    19801 gctactgctg ctgcattagc tgcaattgca ccaagttgaa ttgaataagt tataggagat
    19861 agtacccacc cacttattcc taaagaatat ataaatacta ctaagaagtt taagagtaca
    19921 aatccaggta atgtctgtgc tatatctgat actggtttaa atactctaac tattatttca
    19981 aacatgtcaa agttgagtcc ataaattaat ccccagccta caaacattat cactgttata
    20041 ggtaacatat tatcaaacca cttgcttaca aaatccggaa gtgctgaatc ttctccgaaa
    20101 aatgaaaaag aaccaaataa agacattatt aatccagaga ataatcctgc aactagcgat
    20161 acaagcattc cttctcctcc aaacctgttg aagttaaata tagctccttc attcgtaatc
    20221 tctggacgaa tcatcattaa cattaatcca gcacctgcaa gaccagctac tacactcata
    20281 ctttcaaatt tcttattctt cattatttcc ataggcaata aaaatgctat aaataatcct
    20341 aaaagtccaa atgtatattg atttattgga cttaaatcag gtaaaaagct tatgtaattc
    20401 ttcaagacag aaactatagt tactaatgag cctacaaata ccattggcaa aactttcatt
    20461 atagttgatt gaactgattg tacccatata ttcttagtca tattatttac aacaggagta
    20521 aatttttcac ttaaaaaatc tataaattta ttcataaaca ctctccccat aaatatttta
    20581 tttgtttgat attagttcta gtgcttcttc caaagccttt gcaccatcta gtcttccata
    20641 tgcagtttta gatatacatg ctactggaac gttatattca ttagcctttt gttgaatttc
    20701 ttccttcata taatctaaat gtggtcctag taataaacaa tctatttctg ataaatactt
    20761 atctatctct gactcacttc tcgcttttat atctgcttct ataccttttt tctttgcact
    20821 ctttctcata ctagttgcca taaaacctgt acttgctcct gaaccacacg ccaaaagcac
    20881 tcttatattt ttcataatga tacctccaat atttttatcc attaatttat ttcttaatga
    20941 actttttatt ttattatgtt tatattgtac ttaccaaata agttcatttc acataaatat
    21001 atttcctaat aataggaaat cagttactct taattaaaca tattaatata gtataatgta
    21061 tcttataata cgtacattca attatataat tatcatatca tccagatttt attttaaaac
    21121 tatgcgaggg aatctaatga aaacatctaa aaaagaacta tttttaaatt tcataaaaac
    21181 acaacatgac tggatagatt catctacatt ggctaattat cttaatgtct caacgcgttc
    21241 aattagaaaa tatgtaaacg aaattaattc caatggtgaa tttatattat cctctaaaaa
    21301 aggatataaa gtaaatctca ataataattg tcaaaccaag gttgatagtt cagaaaatat
    21361 atcacctgat aacaggttaa atttaatttt aaaagaactt atagttaata gtaatggaat
    21421 taatatattt gatttaagtg aagaattgtt tgttagccca gctactattg aaggggatat
    21481 agtcaaagct aataaattta taggaagtta taatttaaag ataaagcaat cgaaattttt
    21541 attgaaacta attggaaatg aatctgcaaa gcgcaagtta atgagctcta taatatttaa
    21601 agaaacggga agtgattttt taagcttatt tgatgtacaa aaaatatatc aagagtataa
    21661 tttgactaaa ctaaaagaaa atataatata tatattaaaa aaatacaatt tatttataaa
    21721 tgagtatgct ataaacaaca tacttcttca tttaatgata actatagaca gaataaaaaa
    21781 gaataattac atagactctg ttgaagtagt aaactacata gacaataacg tagatataaa
    21841 tattgcaaaa gacatatcaa attttttaga atctgaatac aatataactt taactagtgc
    21901 ggaattatac tatttagtat ttcagttaac aaacaaaact acagtcttaa actacaatca
    21961 aatggatact aaatcattgt caaattatat agatgagcac tttgttaaat taactaaaaa
    22021 aataattaaa aatgtatatg acttatatct tatagattta tctgatgagg aatttgttgt
    22081 aaaatttact cttcatgtaa aaaatttaat cagtagagcc aaaaataatc aagttttaag
    22141 aaatcaaata cctcaaaagt taaaagattc ctaccctttg atatatgata tatctgttta
    22201 tatttgcaat cagattcaaa ctttagaaaa tgtagatata gacgaagatg aaatatctta
    22261 tatatctctt catgtaggtt cattctttga tagacaaaaa ttattagaag ataaagttct
    22321 atgtgcatta attactccaa attactatga tttacaattc aaaatagtaa gagaccttga
    22381 gaagagattt aatgaatcca tagaaattat tcagattttt tcagatactc ataatcttga
    22441 ttttgacaat aaggtagaca tggttattac tactttacca attaacaata gatgccctat
    22501 accttttgtt tatgtaaatc cttacctaaa tagaaaggat tatgataata ttcaaagtaa
    22561 atttactcaa attaaagata gaaaaaatat acttactgta caaaatcatt tagaaatgta
    22621 ctttagtgaa tctttgttta tgaaaaatat atatttagat agtgctaaag attatataaa
    22681 atttatggga aatattctgt acgaaaataa atatgtcaaa cccaattata tagatgatgt
    22741 tcttataaga gaaaaaatgt cttcaactgc atttaataac aatgtagcaa ttcctcatag
    22801 catgaaaatg gatgctttaa aaactggagt atgtttaatt gttaacgata aaccagttaa
    22861 atggggtgag gaaaaagttc aaattatagc tatgatacct ataaatgaaa aagaaaaaga
    22921 aaaattcaat tacatatttg aaagctttat tgaaatatta tctgagtgga ataatgttaa
    22981 agaacttact aaggcagata actatagtag ttttatgaat agaatagctt atcttattca
    23041 aaatatctaa atcaattaat ttatttccaa tatatgtttc ttaagttatt aacataaaat
    23101 agataagctg tcacactaaa aagtctaaat taatatgtga cagcttttta cctaatatac
    23161 tttgttttaa tactacatat tttttgactg ttgtatacta gttataagat tgaatatttg
    23221 tgttgttgta gctgtcatat tctcactagc agttgcttta tccattgctt cttctattgt
    23281 catagctcta tttactatag aaaaataagc atctataccc tcttcattac atttaactgc
    23341 atcttctgtt gtggctccag ccaaacctat tacttttacc ccatattttt ttgccagttt
    23401 tgcaactcct attggagcct tgcccatagc agtttgattg tccaatctac cttcaccagt
    23461 tatgacaaaa tctgcatctt ttaactcttc ctctaattta atttcttcta atattatttt
    23521 tattccagat tctaattttg aatctaaaaa tgctaaaaaa gcaaaaccta atcctcctgc
    23581 tgctcctgct ccttctacat tagctacatc tttacttaag taatttttaa ctactttgga
    23641 aaaatttcta agcccattat ctaactcctc aattatttct gatgttgcac ctttttgaga
    23701 cccgtatatg tatgcagctc catttttccc aaacagcgga ttatttacat cacaggctat
    23761 tttaaactta cattcatcta attcctttaa tctattttct atttttatat gtcttatttc
    23821 atttaaaact tttccaccta atcctactaa cttgttattt ttatcataaa actcaaaccc
    23881 taaggcttga agcataccta tcccagcatc attagtagca cttccaccta tcccaactat
    23941 aaaatttcta tagcctttat ttattgcttc ctttattatt tccccaacac cataagttgt
    24001 agtgtataaa ggatttctaa gttcagtcgg aaccaatgtt aatcctgatg cttgagctat
    24061 ttctattatt gctgtacttg tattttttaa taatccatat gttgcatcca cttttttagc
    24121 tattggtcca gtaacttcta tagtctcttt tttaccattc atcccttgaa ctaaagcatc
    24181 tactgtccct tcaccaccat cagcaagtgg caatatcttt acttgcgcat cttctttaac
    24241 cttcaatatc ccctttttta ttgcatttcc tgcctctatt gaagataagc ttcctttcaa
    24301 agaatctata gaaattaata ttttcatact tatctactcc tttttattac tccttatttt
    24361 aaacatataa ttttataagc ttaatttatc ttttatttta tactctacat ctctctttgt
    24421 atacaactta taccattaat attcttttat cttataaaaa gggagtgtct caaaataaat
    24481 ttcttagttc atgagacact cttttgtata aaatcaaata tatgtttatt atttcaacaa
    24541 tgataaggag gattatctct attttaagat aattcctttt tagcactatc taatcatttt
    24601 tagcactatt taaccagtat agttttaact tggttcatta agcactctac atttacagag
    24661 ttattatcta ttgatataga ttctaattct ttttcattta ggttaatcca tttagctgtt
    24721 ttaatatttt tgttaaatac tatgttagct gattttgttt cttctttaga attataaaat
    24781 ctaactacta atgcctcttc tttttcagcc ttttttacaa cacttaacac aaggtcctta
    24841 ttagtttcct taagtaaact atatgaataa tctacttcta tttctgattg attcaatttc
    24901 atagcattat atggcatttt attataagtt tgtatttttg ttaaatactc tttagccata
    24961 tttgcaacat ttgcttctag tgtagatttt tcatgagtaa ctattgctaa gtctattttt
    25021 atatctccta tcatttgaga atctggagta ggcaacttta ttcctgatgg acgacctggg
    25081 cgtcttgcca tttcttcttt acctaggaat cctacacttc taaataatgt tatagctatt
    25141 gtgtcaaatt ttttccctac tatttcaaac tctcttgtac tgtttgtaag aacagctact
    25201 ccatgctctt tgtcagacaa tccaacgaat gttagcattg gatatataga atctggtctt
    25261 tcactccagt cttcctgttg ccaaacttcc atagcatcat catacacact tctctttata
    25321 tgtccaaact gattatcaga cacagaaaac tttgaagcta tatttgttgg tatataagtt
    25381 ctaaccctac aatctcttgc ttggttattt atgttgaata ctacatctat tatagcttta
    25441 tcatttggaa catttataac tatatcaaag tcaataaatg aatctgctct attatttttt
    25501 ctagcttgta tatcttttgg tacttttaat ctataattta tctttatctc attattaaat
    25561 ctatttttct ttatagtgga atttgctact atattatcgt taaatattaa tttctcattt
    25621 ggaagtggtg aaaaatcata ctcatctcct tcatctcctc cattttccat caataataca
    25681 ttttcaaaag ttttatttaa ttttttatca aatatcttta atgtcccgtt ttcattaact
    25741 tgaatagtat aaaagtcatt atctaccatg tcacatactt cataagcttt ttctaacata
    25801 tgttcaactt cttttatcat atacgcctta taccccattg caggtatttt atctttaaag
    25861 tttattgtat ataccataaa tgaatcatag tttccataat gaacaatttg acggtctatt
    25921 aaacctgcat ctattacttc tttatgtacc acttcaaaat ccaacttatt attatcttta
    25981 tcaaatattt caaatgcttt cattttagtt ataacttggg cgcttactat ttcattccta
    26041 tcgtaaggca ttaagttaaa tgctactaac ttatctaaag ccatttcaca actcattgca
    26101 tcaactattc ttcttttgta aaatgttatt aattggtcta ctttttcctc agctaagaaa
    26161 aatctattca ttatttcatg gtgaacttta tctgaacaac aacatccaat gctgtcatga
    26221 gcatgatttt tcattatttc tttccaaatt aactctatta atccatgatg atattcaaat
    26281 cctagagaat atgcaataga agctagtggc tccaatatgt tggttatctt tgattctatt
    26341 ctagcatttg ctgattttat atccattctt gttgagaata tacttctatg aaccctcata
    26401 tatttcccat ctaaaaattc cccttttatt gtatctatat cctcattttt ttctagctct
    26461 ttaaatatgt tttcatatct acttaagaaa aattttctct ctggataaca ttcttttaat
    26521 ttatatataa cttcaaatat attcttttga attggcattt ggtcatgtcc atttggaagt
    26581 attatatggt cagtagttgc acccttatca agaaccggta aatatttatc cattcttgtt
    26641 cttagctcat cttcattcat tggtagatat tttcctattg cataacctaa tggaagtatt
    26701 tgaaccaaaa cagaagaccc atcatcactc ttccaattaa actctgtttt attagttcct
    26761 tttctttcag aacatcctct ccaaaacatt gagtatttta tatcaaaccc atttagtatt
    26821 tgtggcattt gagcactttg accaaatgaa tctggtaaat atcctatttt catatattct
    26881 ccaaattcat cacaatcttt tattccatat agtaaatttc taagtatgga ttctccacca
    26941 actaccattt catcagtttg agtataccaa ggacctatta ttagccttcc ttcttgaact
    27001 aattttttta ttctctcctt acattcaggc ttaactgcta aataatcttc taatatagca
    27061 gtttgaccat ccatcacata atatggatag ttatgattat tttctaacat atccattatt
    27121 tcttccatat tatttacaag taatattcta gattcctcag ttgagaagta ccattctcta
    27181 tcccaatgca tatgaggaac tatgtgtaca ttttttttca ttgcttatcc tccaaaactt
    27241 atctgataat attttctgtc taattaaaaa tccaataaaa atactactga gtttacaaaa
    27301 ttttttaata tttattttat cactataatt aaacctttga tttaaacatc taataacgta
    27361 tcttttaatg tatctttctt tgattttttt gtttcctagt tattattaat aatattgctg
    27421 atattatagc tccaagtact gccgcaaaga accatattga tgctataata aatcctgaca
    27481 tacttccctc taaaagcgcc aatgagaata tccccgctcc aggcacttga agacctatat
    27541 tgaagaatgc tactatagac ccagttatgg ctgaaccagc taccaatgat ggtataacac
    27601 ggattgagtc ctctatcata aatggtattg caccttcagt tattccagcc aatcctaata
    27661 tccaagtttg ctttcctatt tccttttctg attctgaaaa taaatctctg cctaaaacag
    27721 tagctattgt aactccaaaa gctgaaacca tcttaactga tgcaaatatt gcataaggta
    27781 taaaatttcc acttgctatt gctcctattg caaatgtata ggctgcttta tttattggtc
    27841 cacctaagtc aaatgaaacc atagctccta ttattgcacc tagtagtatt gcatttactc
    27901 cagtcatccc atttaaccaa tttataagac cattgttaag tgctgctaca ggctttccta
    27961 ctacaaataa catcaataaa cctactacca aagaaccaac tactggatat acccaaaaac
    28021 ttacgaatcc agctaatgtg cctttggctg gtatattctt ctttaaaaat tttactatat
    28081 aaccagcaag taaacctcct aacatacctc caagaaatcc actacctatt aaatcagctg
    28141 ctaaacctgc tgcaaatcct ggtccaagac ctggtttttc agctagtgag taagacatat
    28201 atgctgataa tactggaacc atcaatgtac ctaacatacc tccagaaagc tttcttaatt
    28261 gccataacca agaaccttct tgattgtata gttcttgtag accaaatgct tgagatgcta
    28321 atactgcaaa tgcaagtatc atacctcctg ccacaataag tggtattata tgagatactc
    28381 ctgtaagtat tgctgatttt acatcttttt tccatgatga tttttcacaa tatgatatat
    28441 cagatgattt ttctatataa tcacctttac cttgtttttg tgctacttct aaagcttcgt
    28501 atattatttc ttttgctctt tttagtggag ctgctactga cgtttttatg tttggcaaat
    28561 gttcaaatct ttctgtattt ttaggtgcta catctgttgc atatattatt gcatctgccc
    28621 tttttattat atctggtgta tgagggtcca ccataccatt agctccttgc ttttctacat
    28681 acacatccac accgatttct ttaccagctt taattaatgc ttctgctgac atatatgtat
    28741 gtgctatacc tgcagggcat gctgttattg ctaatataat tttttttgat tcattttgaa
    28801 tcctaatctc ttcttttatt tgttctttct tttcttttat atctaagtta ttaaataact
    28861 ctttcttagt ttttgaattt aacaacatat tcatataatc ttcatttgac agtctagtaa
    28921 ctaactcaga aagtatttgt atgtgtgtat ttccaccttc attttctggt actgctatta
    28981 aaaatattat ctctacttta ttatttgggt ctacgctttc ccaatcttct attggtgtct
    29041 ctaaggtcat agctgcaaat gcagcttctt taactgactt agattttcca tgaggaattg
    29101 caagccctct ttcaaaacct gttggagata aagtttctct gtacattaca gcttctaaat
    29161 attcctcata agatgttaac ttgccttcat catatagctt tcttgctaag aactttattg
    29221 cgtctttctt tgtttttatt ttttgtttta aaaaaacaag gttttcattt gtgattttct
    29281 taaattccaa aattacgccc tccttaaatc atttaatgct atgaatatag cataaaataa
    29341 attatgaaaa tgtttttttg aaattataca cacaatttct tcttaaaaaa taaagatttg
    29401 tgtatttata cacaaatctc tatcttaacc agtattgctc tattattgct ctcataatca
    29461 tcataattgg aaccttatct gatatattgt acccatttac ttgtttaatt gttgaatagc
    29521 aatataaact tatatctgca atttgttgca attgattttc acatatatgt gttagagcta
    29581 ctaccttaca acccttatta actgctgtct ttgctgcttc tataacttct ttcgtttctc
    29641 cacttaaact aagtgcgaaa aacatatctt gttcacctaa ttttcctata tcatgaagta
    29701 cttcatgtct ttgactatag taagttatat tatcatgccc acctctaatt tcttttacta
    29761 atatctcaca taaaggaatg ttttgaccta ttccataaac tataatactc ctagattttt
    29821 gaattatatc tgctacttta tttatttttt cataatctat tagctctaat gtctttttta
    29881 tatctgattc aaaaccattt tttggctctt tattctcatt ttttaactct tcttttaaat
    29941 tattttttag ttgagagaaa ccatcatact ccattttttt acatagccta actatggtat
    30001 ttgggacagt aaaaaattta tctgctaaag attgtattga taattttact atttcttctt
    30061 tgttttttct catatattca attatcatgt cttctgtatc tgtaaattta tactcatatt
    30121 tttttgctct ttcataaaat tccatagttt ctcctccatc tattttatta ttcataaata
    30181 taatactctg tattatctaa attttttagt aatattttta attttttcac atatattata
    30241 ccttttatta tacataaaaa ataagtactt cctatattga tggagataat ttaaaagtgt
    30301 gttgtttaat atacttagtt atcttttgaa tataatcgat ttgacattat cattaacaga
    30361 tattagaatt taagatagat taatttttat cgcaacttga taaatcataa cttaattcta
    30421 tataagtgag gtgaaaagag tatgtttaca gatataagaa gcaaacgagt tatatttgtt
    30481 gcccattgtg tgctaaatca aaattctatt tcagatggta ctgctgatta tcctagcaca
    30541 aatacggatg ttgttcgtat tctaatatat ttatgttatt aacaaggatg taaagcctaa
    30601 ttttacaaag gttctacatc ctatattcat ttacagaaga tgcatttgtc aaaaaaatcc
    30661 taaaattttg tcagcaaact aattatatat cttgctattt tattttgatt tacacaatat
    30721 ttagttaata taaaacctta tatgttgata gaaaatcaac atgaccttta gatttaattg
    30781 ttagttttta aaagctcttt aaaaggtctc taaaatcaac caactcaata ttatttcttc
    30841 ttaaccatat tttaacttcc ttatcacata gcattgcatg ttcatatact ctctcttgaa
    30901 taagagaaga tgattttata agactataat ctataaatcc tggatgagta accctgaacc
    30961 tcccatgact aaagtcacag ggttcctgct tcatagaatt ttgcatacta aaatatgtct
    31021 tactaattct ccacaggcta tccccgtagt tcctacggtt cttacataag ttatttattt
    31081 tgaattgtta ttagtcttaa tccttctttt aatatattta tacttgcatt tatatctcta
    31141 tcgtgagttt cattacaact aggacaaatc cattctctta tatttaagtc cttcacttcc
    31201 tcgtttttat acccacattt attacatatt tgtgaacttg caaagaattt acctaccttt
    31261 acaatttttc taccatacca attagccttg tattctaatt gacgaataaa ctctgaccat
    31321 gacacatcag aaattgagcg agatagttta cgatttctaa tcatattctt tacttgtaaa
    31381 tcttctatgc aaataatatc attctctttt attaatttag tagatagttt ttgtagaaaa
    31441 tcctttcttt gattagctat atgttcttga agtcttgcaa cttttaatct tgctttattt
    31501 ctatttaaac taccgattgt ctttctcgat aattctcttt gtaatttagc aagtttgttt
    31561 aatgacttct taagatactt aggattttct ataaattctc cacaacttga gatacaaaat
    31621 tctttaattc ccaaatctaa acctatatga ttattagtat tttcaaatgc ttcaatatct
    31681 acatcagtac agcataatga tacataatat ctaccacttg gttctttaga tatagtagca
    31741 ttaagtattc tacctttagg tacttgttta tcccttacct taaccatacc aagtttaggt
    31801 agttttatat gttgaccaca atacacgata tttccatttg taaagttagt tttatatgaa
    31861 aatctattaa ttttctttga tttaaattta ggaaatcctg cctttttttt aaagaatttt
    31921 ttatatgcat tatctaaatc ttttaaggcg ttttgtagtg aaaatttgtc aggttcctta
    31981 agccatttta attctttttt taaattggtc aaatcagaac tacactgttt ataagtaaaa
    32041 gtttctttat cgtttttata aacttctatt cttttagcaa gatacttatt atatacaaat
    32101 ctacaacatc caaaagtttt gttaattaac tcttgctgtt ttttatttgg atacattcta
    32161 aacttataag ctttttccac tgctatcacc tcactttact tgttttttct gattattaat
    32221 atattttctt atctgctcct ctgtattttc tgatacagta gcaacgaaat aactaggatt
    32281 ccaaagatgt ctccccataa taatggatgt ctatatttga catactatat gcatatcctc
    32341 taccatgagt aacttccatt tttcatcact tatcataata ttaaaatata caattgctta
    32401 aaacaagtat tttgaagttt tttatattta tttttaaata acatatataa gaaccgtaag
    32461 tcttaacttt tatcattttt gaggttgtcg ttcacataag ttcgctacta cctatgcagt
    32521 tctcttatga acttcttaca ctttcatgta agcacagact atatcttatc cttcggcatt
    32581 atccgctaag gtctacccac ttccactacc aatagcttgt agtgtacttc cctcaagagg
    32641 aatagtcgtt gaagtttctc ctattcggag cttacctgct gattgcccat tttaaagtac
    32701 ttaggattta accttatacc atctaaccaa ttttttctac tttcgtaact ttcacactta
    32761 ggtttatttc atccttatgt tttagtttgg ttagctttag gggtttttag caattcaagt
    32821 agtattggat aggttctatc ctatctctac atataagttt ccctatatgc tgactatttt
    32881 agctactaac tcatgactaa agtcacgagt gtgcgtagct attttttaat caatgttata
    32941 tctttatcta aatttatata tttttcttca aataatttaa caaaagattt atttaacttg
    33001 taaaaatcat tctgaggata tttacttggc atgctaatac atgaatccaa ataataggga
    33061 caacttggta taccatattc ttttgcagca tcacacattg ctgatattga tacatcttct
    33121 tctataccat gtatatcaat atactcaggc aatttattca tatacaactt gtaagtttca
    33181 atttgagcac aaacttcttc atatacttct tcataattaa aaacctcttt acctgtcagt
    33241 agttgcgttc ttcttatttt gctagaaaca aattttccac cttcatctaa caaattcttt
    33301 atcttaatat gttctttgca acaaggttct cctattacta aattaacatg taaaccaaaa
    33361 catacatcat cattttttac catttctata gcttgtcttg aaaaaggcat attcaccatg
    33421 attccagcac ttgtaacaat accgttctta taagaagata ttatccctag tgtaacacct
    33481 tctgaatatc ctatatcatc tgccctaact actaatttct tcattttatg catctgcctg
    33541 ctttctcaac tcatcaagtt gcatttccat ctcatccttt actttaacaa catccgttcc
    33601 tatgatgatt tgaactgatg tattactcaa tttaattacc cctttggctc ctgttttttt
    33661 aatattatca tcattcaaaa tagtagcatc cttcacttct aaacgtaatc tcgtcataca
    33721 attattaaga gtcactatat tttcataacc accacaattt ttaattatct gtttagccat
    33781 atatgaaaaa ttaccatgag aaagttttaa attcttttct ttttcactaa ctttatcacc
    33841 tatctctatc tcaacttcac gtcctggtgt ttgcacatta tcttttatta ttatatattt
    33901 aaatgttaca taatataata caaagaatac aactcctaca ggtataacta accatgcttt
    33961 atctccataa ctaaaactta agacatagtc aattatactt gctccattaa atgaacccaa
    34021 gcgagcaccc agtaaatata gtacaactcc acctagacca gaataaaaag aatgaattat
    34081 atataacttt ggagatgcaa ataaaaacga aaattctatt ggttcagtta ctgttgccat
    34141 aaaagaagtc aatgctccag cggtcattgc accttttact tcttgcttct ttttatcttg
    34201 tgctgtttta tatatagcat aagaaatagc aggtattgaa aacatcataa ttatgtaaaa
    34261 tcctgtccag aagaatcctg catttgggtc tcctgccata aatctaggaa tttctcctct
    34321 tataacttct cctgttgatg ttgtatattc acccatttca aataatatgt aagcatttaa
    34381 aacttgatgt aacccagttg gtattagtgc acggttacag aataaaaaca agaatattcc
    34441 tattgcacca gctcctccca accaaaaacc aaattgattc attgcagctt gtggatatat
    34501 ccataataaa ctaaatatat atgataaaat taaagcaacc actggtgata gtgtaattac
    34561 acatttttct cctgcaaaga agcttaaaaa tgctggaact ttaactttat atgttttatt
    34621 gtaagtccat gctatacaag ctccaacaat tatcccacca aatacacctg catttaatgt
    34681 atcattcaat aactctaaat ttgacttaaa tatcatatat gcaactacgg ctgcaaccat
    34741 tacacttgtc ttgtctttag actttgagta ggcactagct gctcctaata aaaataagta
    34801 gtccacattt gaaaatacta tattagatat attagatata gctggcacat tgagcatatt
    34861 tggaccaaat aataatccta atatacctgc tgctggcatt acagctattg gcaccagcat
    34921 agtctttcct aaaagtgaca gttgttgcat cattttgttt aagaacctct tcatatccaa
    34981 tcccctttct atttcggttt aattttttgt gaaaacgatt tattcacatt ttatatttta
    35041 aatattatat ttgtttaaac aaattgtcaa tattttttat tcattttatt tttatcattt
    35101 agtttaacat ataaatcctt ttaagtgtat tgctatatct agtctatttt tttaattcat
    35161 tttttttatt ttaaaaatat gtttaaccat gtatttgaaa caacccacaa aataattcta
    35221 ttttgtcctt tttatggttg atatgattct attttcacta tacatgtcta aacacatttg
    35281 atataatttg ttcatatcag ttcaataata ttactaaaga cctttaagga gggatagggg
    35341 tatatggcta aaaataaatt ttcagagata tatgaagtat tgaaagagga aatacttgat
    35401 ggtaaatata caagtaatat gatgctgcct actgaacttc aacttattga aaggttttca
    35461 tgtagcagaa acactgtacg tagagcaatt tctcaattaa acacagaagg atatgttcaa
    35521 agtataaaag gcaagggtgt tgtagtgtta gaaaatagtt gttctaatga tttttttcta
    35581 aatatgcaca atttcaaagg tgtagaatcg attgtagaag ataaaaaggt taatactgct
    35641 actagtgttc tccacttcag caaaatctta attgataata agttatctaa aaagacaggt
    35701 tttaaagttg gtagtgaagt atattacctt cacagactaa gatatattga taacatacct
    35761 aaaattctcg acattaacta ttttctatgt agtatagtta aagaccttga tgtttctata
    35821 gcacaaggct ctatttataa atatatagaa gagtgtctag gtacaaaaat tgtttcatcc
    35881 agaaagatat ttaaaattga aaaagctaca gagcttgagt taaaaacgct acctctaaat
    35941 gattataatt gtgttggtgt tattaaaaat agtgtatata ctgatgatgg aaagcttttt
    36001 gaatatactg aatcaaagca tacacctgaa acatttgtat tcatggatgt tactcaaaga
    36061 tattaaaaga ggatgtctca aaataaatat ttgaatcatc tttttttatt ttaccaaatt
    36121 aaaattattc attttagaaa tcaatttttt atcaattgaa aaatagatag gaacaaattg
    36181 tccctatcta tttttaatag tttatttttt aaactatttg ttgttaaagt ataaatagtc
    36241 tatactctgt ttaaatcttt caagaccttt ttttaatttt tctcttggac agccacaatt
    36301 taatcttaaa tatttctcac ttccatatac ttctccccgc attattccca ctcccccaat
    36361 attaattaaa gaatcttgta tttcttcaca agtaaatgga acgtctctac aatctatcca
    36421 tgccaaataa gtaccttctg gctttgtaaa tttaatatct tcaaaattct cttttataaa
    36481 ctgtgataca taacttaagt tattatctat gtattcgacg agttgttcta tataataatc
    36541 acattcatta taagaaacca ttgtcgcata catacctggt aaacttacag agtttagaaa
    36601 atctcttctt cttgttattg ataaaaattc atcatatata tttttatttg ctataacagc
    36661 atatgaacct attaaccctg gataattaaa tgttttactt ccagaagaca ctaaatatag
    36721 ttctttatat tcccttaaat atttaactat tggcaaatgt ttattatctg gcatgattat
    36781 atcagaatgt atttcatcac ttataatttt tatattgtat tttttacaag tatttattaa
    36841 aaactctaat tcaacttctt tccaaacctt acctgttgga ttatgaggtg agcataataa
    36901 taatacatca caatttttta cactatcttc aaattcgtca aaatctatat taaatttacc
    36961 atccttttca tataattgta ttttttctaa ctcaaatcca ttttcttcta ttacactgaa
    37021 aaatgcatca tacatagggt taaatgtagc aactttcttt ttattattac aaagtaatct
    37081 aagtagtaat gctactgaat acataactga tggactatat aacacccaat cgctctctat
    37141 tttcgcttca aatcgtctat tataatagcc tgtaatactt cctttaaaat cattgtgatt
    37201 ccatctagaa tatccatata tctgatgctg taatatctcc tgtaactttt catatactgg
    37261 tttaggtact gtaaaatctg tatctgaaat agaaaatgga atcaaatctt ttttaccaaa
    37321 tctatcttgt atataatccc actgtgtaca ataagtacct ttgcggttat aaacttcatc
    37381 aaaattgtgc ttcatattaa tcctccaata taattcttct cacatacaat ataaaaatac
    37441 gatttattag atttttatgg agtatttttt gcttcatttg catgtttttt atctaaaata
    37501 aaatattaat tatattttgt tactaaaata tctttctact ctataaaata aaagctatga
    37561 atatagattt gtaactctat ctccacagct tttatttaca tgcttgatta tatttctaga
    37621 aatctaaatc acctaataag cctaaatctt ctggagatat tacagattct tcttgaattt
    37681 cttgaacctg cttagtatct ctctcatcac actgtttttc atacatttta tagaatggat
    37741 accatattaa tatagaaccc accatttgaa atgcttgaac tattggagtt aaccaatgtc
    37801 catttactaa gaatccttct ataaatactc ctaaatatgg agggttgaaa aagggtttgg
    37861 ctaatattcc tgcattcatt aaaaatgcag ggaatgttcc cagtatagtt cctccaatta
    37921 caaaaggtaa aaagaatgtt gggtttaaaa ctatcggacc accaaataat ataggttcat
    37981 ttataccaaa taatgctggt actaaagaaa cttgacctat acgcttgaaa tctactctct
    38041 tagacattaa gtaccaaaat accaagccaa atgttactcc tgtacctgta aaatttgcaa
    38101 atgcacttac cattcctcct gtataaaagt gtggtaatga ttgacctact gccatagcct
    38161 ccaagttttc agctaaaaat tgagctgtta ttggtgctgt tattggcgat aatacagatg
    38221 gatgtattcc aaaaaagaat aatgttattc ctagaaatgt tataaatgca aatgtaaatg
    38281 gattatctaa agaaccaact aatggagcta aaaattgggt aagtatttct ggaggaagta
    38341 ctccaaatac ttgtaaagtt attattctta tacctataaa tactccaact attattactg
    38401 ttgtaggtat taattcaaat gaacttgata caaaatcggg tacactttct ggcatacgta
    38461 tagtaaagtt tttcttttta caccatctat acaactcaac agctaataca cctgccacca
    38521 ttgctataaa taacccttta gttcccatat acttaatatc cattccacca tcttcagtga
    38581 aattaaaact cattaaaaga aaaccaacag cagctaagga cattgctcct ggtacataaa
    38641 gcttatagtg ttctgaaagg aaatacgtta tacacataca tactaaaaat cccatcattc
    38701 ctatagttaa gtaaaatgga atttgtaatg aatccatatt atttgttata aatgaagtta
    38761 tagcatttgc tcctatagct ttttctgtta agtttcctat agcctctggc ataagactta
    38821 aacttcctat tataaggatt ggagtcatcg ctaccattga ctttttaatt gcacttaagt
    38881 gatgctgttt gtccattctt tgtgcaatag gtgtaagatg cttattcata aattgttcga
    38941 actttttaaa cattattttt cccccatcat catagtatac tttaatgaat attacatatt
    39001 agcttttttg tgagctatta atgccatctt aagtgcagtt gcaccatcca tttttccata
    39061 tgtattttga tctattatta catatggtac atttttaggc tctagtatag gttttatgta
    39121 atcaagttta tgagctattt gtggtcctat aactagtaca tcacaattat caacctcatt
    39181 ttttaataaa tctactggta ttgctttaaa ttcaaaatca tcatcattta attttttact
    39241 attttttaca acattctcca tatttttcat tagcattgta gtcgaaaatc cacctgcaca
    39301 acataatatt attctcattt ttacgcctcc ttagattcaa atatatctat taatttttct
    39361 attaaatctc tagataactg tgaagtcatg taatgatctt gggcatgtac cattaataaa
    39421 ctcaccattg acttactttc acaatctcca gataattcac tttgtattaa tgcagtttgt
    39481 gcattatgag cttctatatc tatttttcta gcttcttcta tacaagctct agctccttca
    39541 taatcgcctt ctttcacttt atttaaggct tcaaatgact ttgatttact atcccctgaa
    39601 gaaactatta tctccattat tttcatctct aaattttcca tgcttacctc ctatgtcgtt
    39661 aaataaactt taatatgtta ttgtacttct ccatattcat atcattaata acagcagtac
    39721 ataaattgat aacctcttca acatctctca tatctaccat tgaataattt ccatgacagt
    39781 atcttaaagg aacccccaaa actatagcta attttccttc atttgctaga tgtgcatttc
    39841 cagcatctgt tcctccacca ctaaacatat cgcattggaa ttttatatca tacttagttg
    39901 ctaagtcttt catataattt aaaaattttt tattaggaat cattgtttta tcataatgca
    39961 ctatcataca acctttagat aattggcgtt gattggtata atttctaatt aaatctggtg
    40021 cacaagcaac atctacagca aacactatat caggattcac tacttttgat gttgttttac
    40081 ctcctcttat tccaacttct tcagaacttg tatatacaag atatatatca ttatcattct
    40141 tttttatttg attaactctt ctatctatct ctttaagtat atagcatcct attctatcat
    40201 ctaaagcctt tgccataagt acattttcat ttaacatttc actctcacta gcaaaacata
    40261 ccatatctcc tatgtttata cctaaatcct caacttcttt agctgtactt actcctatat
    40321 ctgcatataa atcttttact ttgttatttt cgtcatatga acaatttata agccctttta
    40381 ccttagtacc atcaacttta cttatattta ctttttggaa agactttgca ctatctttaa
    40441 ctccaccaac attaattaaa tgtagcattc caatatcact tatgtgcctt accataaatc
    40501 caacttcatc catatgagca caaaacatca tttttaatcc attacctcta tggtgaacta
    40561 tcatacttcc taagttatca aaactaattt ctccatttat attttcagct aataggtctc
    40621 ttatttcttg ttcatttgaa gctattccat ctgcttcaca tagttttttt aaataatcta
    40681 tactcatacg attaccctct atattctttg atttattaaa gctacaaatt ctttgaatgt
    40741 tttagcattt acaagtagtt gaacagtttc tatatcattc attatttcat ataaactact
    40801 actaattttt ttataaagtc caatattttc tttttttaaa caaatcataa atattaaaga
    40861 aactttttta cctttaaact ctattggatt ttgtaaaatt tttactgaaa ttaaattttc
    40921 atttccatca atatctatag gatgaggtat tgcaactcca tttatatata tatttgttgc
    40981 aaagtactct cttttcaaaa cattttcaga gtacccataa tttcctgcac cttttaactc
    41041 tatattttta gacatatctt ctaaacattt gatataatca attgaatcat tgtctatatt
    41101 aaaatagtca tttcttaaaa atttgcagat aaatgcttga cctttatttt ctaaagagta
    41161 ttccttaaat ttttcaaaca taattaactc tttgattctt aaaatatcat aatcatctaa
    41221 aagttctttt atatatacaa ttggtacttc tagctttata tctagttcac aaatcgaaaa
    41281 gataatatct ggattaaatt tttctaattc tttcatctgt ataagagaaa aagtttttat
    41341 attagtattt tcaaacaaac tgcttatctt aaattttatt aactgagcac ttccaccacc
    41401 tgatgaacat actattgcta ttgaattata ttttctaaat ttatagatag cttgcttttc
    41461 catattcata gcaaaatatg tagctataaa cccaatttca tctcgttgga tttcaacatt
    41521 atacttttta tacattattt ttcctagtaa tatagctata ttaaaagcaa ctggatattt
    41581 gatttgtatt tcatctataa gcggattttg ataagatata ctaaaagtag acctttctaa
    41641 taattctgtc aaatgattta ataatgacct tttaaattct tcatcttcat taaaatatgt
    41701 attattttct tcatcaaatt gcttcaaaaa atcttctaaa tcatctctta agttatctgc
    41761 acctttttgt cttctaaatt tttttctagc ttttttcttt atcaaagatt tcaatatctt
    41821 ataatcttca ctagataaat ttaattcata ctcattggta ctatcatcta actctaataa
    41881 tttagattgc ctatctatat acatagttac ttttaaccaa gacagtattt ctttcaattc
    41941 aatataattt atgactaaat tattttcttt taatttattg ataaatttct cctgaacttc
    42001 tttaaaatcg ttttccagtt tactatcaat atactttttt ataatttcat tttcttttat
    42061 ataaagctct actatcaatt ttcttctttc tttcatacta tttataattt tcaatccata
    42121 gtgagatttt ctttctagct taagattgta tttttttaaa taagaatcaa tatcttcttt
    42181 atatcctttt actactgacc tacttgtttt taattcttct aatatcctat ctatagttat
    42241 ataatcttca tttaatataa gacatgctat tatactttca acatttaact taggttggtc
    42301 taaatactca aataactcaa ctttaaattt tttaaataaa gtattatctt caatctctaa
    42361 tagataacct aatcctcttt tttgtattat tttaaaacct ttattactag ataccttaga
    42421 taaatacgaa aaatcatttt gtagtgttct tttagataca gaatacttat ctataaaata
    42481 agctatagat agatacttat ttgtgttttc taaatcattt ataattttta atattcgcat
    42541 tcttacacct cctattatcc ataacagttt tatttttata ttctaaaaaa tattgtagaa
    42601 ctattttatt ttaattgtac aatctagtat cgatttgtgt aagatgctta tactgttatt
    42661 atattttcta tacaatacct ttaaaaggtt tttaatttca ttataaaatg aaattaaaaa
    42721 cctaatacat tacctattat tgaagatatt gtgtatatct actagaattt aagtatcatc
    42781 tattatttgc atttaataat acaacaagat attttttgaa ataagagtta aataatagta
    42841 caacctttta ttgcatttaa atattatcaa aacaaaaata aatgtactat actatttatc
    42901 ccatactaac ctcactattc tatatacaat tatatgttta tacaaataat catttatttt
    42961 atattttagc tttaatatca gtttaatact tgacaatttg tttaaacata ttataattaa
    43021 cttataaatt acaggaggta tcaagtatga aaaattggtg gaaaaaagca actgtctatc
    43081 aaatatatcc aaaaagtttc aaagatagca acaatgatgg tataggtgat ataaatggta
    43141 ttatagaaaa attagattat ctgtattctt taggtgttga tttattatgg ttgactccta
    43201 tgtacgtttc tcctcaaaga gataatggat atgatataga agattattat aatatagacc
    43261 caaaatatgg gacaatgagt gactttgaaa aactcttaaa agaagctcat aaaagagaca
    43321 taaaaataat gatggatatg gtcctaaacc atacatctac tgaacataaa tggtttaaag
    43381 aatctaaaaa aagcaaagac aatccatatc gtgattatta tttctggaaa gatgctaaac
    43441 ctgatggttc tgttcctaat aactggatat ccagatttag tggaactgct tggaaatatg
    43501 atgaaaccac taaccaatat tatttacatt tatttgaaga aactcaagct gatttaaatt
    43561 gggaaaatga aaaggttcgt gaagaatgtt acaaaatact agaattttgg gctgataaag
    43621 gtattgatgg ttttcgttta gatgtagtca acctactctc caaaacacct ggattacctg
    43681 atgaccctat aacaggacca aaaggagatg gaagaactca ctacgctgat ggtcctcgta
    43741 tacacgaata tttacataat atgaatcaaa aagttttcaa acctaaaaat atagttactg
    43801 taggtgaaat gtcatctact acacctgaag agtgtatcaa ctatactaga gaaaatcgag
    43861 aagaactatc tatggttttt agcttccatc atatgaaaac agattataaa ggaggcgcaa
    43921 aatggactaa tgaaatgttc tctctggata aattaaaaaa agcacaatct gactttcaat
    43981 ataaaatgta tgaaggaaaa ggatggaatg ctctatttta ctctaatcac gaccaaccta
    44041 gagctttatc tagatttgga gatgatagat tttttcgtca agaaagtgct aagatgttag
    44101 ccataacatt atttggtcta caaggtacta catacatata tcaaggtgaa gaaataggta
    44161 tgacaaatgc ctactttact aaaatagatg aatatgatga tgctgaaagt aagaatgcat
    44221 actatcatat gattaaatct ggtattgatg aaaaggaggc attgcttata cttcaacaaa
    44281 aatccagaga taatgcccgt actcctatgc aatgggataa tactatttat aaggggtttt
    44341 ctaatcataa accgtggtta aaagttaata atgacaatat tagtgtagaa aatgagctta
    44401 atgattctcg ttcagtattt tatacttatc aaagattaat caaatatcgc aaacaatatg
    44461 atatttttac agaaggtact tatagattgt tagatgaaaa tcataaaaat ctatttgttt
    44521 atgaaagaga gtataaaaac caaaatcttc ttgttataag taattttact catgataatg
    44581 tagtatataa attacctgaa acttatttga ataaattaaa ctacacaata cttgaaacca
    44641 attacactag agatataatt gaaaataaaa tggaaataaa accttacgaa agtataatga
    44701 tatattataa ataaagttat ttatgtaaaa atcggtataa caccctggtg ttataccaat
    44761 tttactaaat tcaaagtaat atttatagaa tctctataga ttacaataat tatttgactt
    44821 ctacatcatt atatttcttt gaatatactt tagtcatata gaaatatata ggaactacaa
    44881 ttatcatccc acctaatccc caaactatct tcataggttc atcaatacct gcttttatca
    44941 gtaataatat acttataata actgcaaata ttggtataat tgctcctcca ggtattctaa
    45001 aatttacatc tttaccagca tacttctttc tcaatattag tacagctaga catgtaggaa
    45061 tatattgaat aaatctagat acaacactta tttgagataa tgttgtgaat gaacccgacc
    45121 atgctattag taaagttcct atgacagata ctataattgc tatataagga gctccatttt
    45181 tattagtttt tcttatagac ttaggcatta atccttgttc aaccaaagca gcagcagacc
    45241 ttggtgtaaa tatggatgat gcaatattta tacctccaac agatactata gttcctattg
    45301 taattatata cccgcctata ggacctaaca tcttaatggc agcatctgca acaggagtag
    45361 ctgaccctgc taagccatta cccaacaccc ctatacttat tcccagtata agcatatata
    45421 ttactgatac tacaaacatt accattacca atgctttagg tacatttttc ttagggttct
    45481 ccatatcttt tgctgcaaca gctaaagatt caaatcctgt aaaagcataa aataaagtta
    45541 tagatactgc tacaaaagct gatgacgcac tttgaccttt ttgtattatt acaaatgggg
    45601 taaaattaga accctttata aagaataatc ctacagctat aaataaaact attggtaata
    45661 attttcctat agtcatgata ttatttaagt gcttagattg tttcattccc ataaaattta
    45721 taatcccaag tcctacaact aatataccaa ttattatatt tttaataagt ggttctgttg
    45781 cttgtggcca tacagatgat agtgttacag caaagaaatt tgccatagta gcccaagcaa
    45841 tcatactaac tatccacttc ataaatccaa cttcaaatcc aacaaaatct ccaaatgcct
    45901 cttttgcata aagataggga cctccatcag tatcaaacca actacctgct tcagcaaaac
    45961 ataaagcaat agaaacagct aaaagcatac aaaaaactaa tactaatata cttgctggac
    46021 ctatatctgt ataagcatca ccaggtaacc cgaatatacc tgaccctatt attgcattga
    46081 ttcctaatag tacaatactc caaaaaccta acttaatgcc tttttccatt gttattcccc
    46141 ccaaagccaa cttaatatat ttgtattttt tatttagatg cttctttaac tattgccata
    46201 aatatttttt tcatattatc ataatctcta gtcatcattt ctggatgcca ttgtatccct
    46261 attacaaact cacttccttc cttttctata gcttcaatta aaccgtcttt agatgtagct
    46321 gaaacaatat atcccggtgc tactttgttg acaactaagt gatgaaaact attaactaaa
    46381 acttcttttt ctcctagtat ttcatataat tttgtcccct cctttatctg tactgtatgt
    46441 gtaggtacat tagatagatg ttgctgattg tgtttaatat aagcaccttc aattaaagat
    46501 aaatcttgat acaaactacc tccttcagtt acatttataa tttgttctcc tctacatata
    46561 cctaaaactg gttttttcat atctaaagca tgttttaata atttcatatc aaaactatct
    46621 ctctttggtg atattgcctc caacttatta tgaggttctt ctttccatat gagtggattt
    46681 acatcttgtc ccccagatag tataagcgca tctatattag atacctgctc ctttataatc
    46741 tcatcatcat atacaatcgg tacaatatat ggtatagctt tacacataac tactgattgt
    46801 atataatcat tatttacata agccctttca tagccaggaa acattcctcc ttcatcaatt
    46861 agtaaattac ctgatattcc tattattgga tatttctttt tagccaattt cttcacccct
    46921 taacttattt gtgaacaata aaaaatgcta cttctcttaa agagcaagta gcataccaag
    46981 attttattta tttgaacatt ataatttatt ttagaaatac tttttttgaa tacgagtaat
    47041 ataaataact ttatttactt atattttatt ttaatcctgt ttagtttgac ttttttaaat
    47101 tgttttaagt acaactaata ggtaaaatag gcatttagat tttccttttt atcaacctat
    47161 taaatactgt ttaatatatt ttttcccctt ctatttaatt cacttccctt tctaccagat
    47221 tcacatatta tcaaatccag ctcttttaac atattaagta ttccccttac ctcaccttct
    47281 gttatattgt agcctaattc atttattgaa ttaacaatac catttctacc tatattttta
    47341 ttaagattat tggcattttt taaacactcc attactaact ttactttctc tatagatgtt
    47401 ctatccttta atatacttat ttctttacta aaatcttcag ttatatttaa taagttatga
    47461 ggtaaatcat ttattaaaat aacatcctca gaactcataa tgttaatata catagcagta
    47521 ttttttaatt ctcttatatt acctggccat tcatatgcat taataatgtc ttgaacatct
    47581 tgactaattg ctctcttcct tttcataaaa tgctttaaca taacttctat atcttctttt
    47641 cgctctctta aaggtggtat atttatgggc aatacattta tcctatagta taaatctgct
    47701 ctaaatctat ctccttttat catttctaat aaatttttat ttgtagccgc aattactcta
    47761 acatctatat ctattacatt ttctcctcca accctcatta cttgattctc ttgtaatact
    47821 ctaagtaatt tgctttgtaa gtatataggc atgtctccta tttcatccaa aaatatagtt
    47881 ccattgttag cctgttcaaa taatcctttt ttacctcctt ttaaagctcc tgtaaaagca
    47941 ccttcttcat atccaaaaag ttcactttca agtaaatttt ctggcatagc tgcacagttt
    48001 atcgctataa atggctgact atgtcttggt gatgcattat gaatagactg agccatgagt
    48061 tcttttcccg ttccactttc tcccgttata agaattgtat aatcagattc agctattttc
    48121 atacctaatt tcttagttct ttccatcttc gttgaaactg tttttatatc atcaaaagta
    48181 tatctagcaa cttgtccctt ttctctaagc ttctttgata ggttttgctc caattttctt
    48241 atgtaagtta tttcttgaat acaaaaataa tatccagtaa cccttccaaa actctctata
    48301 ttatacttat ttacgtttaa gtacttatta ttaaatttta taacttcatc atgtgcctta
    48361 ctcaaactaa aaatcttttt aatattctca ttctctaatt catctatatt tttccctata
    48421 atattacttt gaatatctag tatatcccct aaactattat tgcaaataat tatgtttccc
    48481 ttatctgata ccattgccat acctaagttt gataaattta ttatagaatt tagttcatct
    48541 gcttttatag taagttcttt gtatttagta tttattcctg aatataaact aattatttca
    48601 tctgtatact taattagcct ctcagtaatt tctttatcat ccagtttgag ttttgctatt
    48661 atttgaataa acgttgaact atcaatatac ctatggccta aatcaataac ttcttctata
    48721 tattttggaa ctaaacttga ctctcctggc gttatagcta tcttaatatt tctatattct
    48781 tcattgggat tgtatggtat aaaattcaaa tggtttattc ctatattgta taaattagat
    48841 attgtttcca atacagtatg cttattatcg tttactacta atacatctat tccttctggc
    48901 aatgaaaata acttatatat atccttttgc tttatgctcc tatttatagt tattatcttt
    48961 gagacatctt ctacatattt ttttattttt aatactctat catctatcat taccaatatt
    49021 atgtcatctt ttattaattc cttctctgtt attttattta aataataatt ttttatcatt
    49081 actttatctt taaatatcat ttctagattt ttatttaaga aacattctaa tttagacaac
    49141 ccatcagtta ctactgctat tgttttcatt tgattactcc ttttaaaata tactctactc
    49201 ttttataata tacatctgat tttcacttta acagcacaaa aaattgtaca tttagtatac
    49261 taacatattc ctttattagt tttatgtccc taaatatcat agttgcaata tctatcatat
    49321 ttcctaaact tctatagagc aaaaattgtg ccaaattaat aattttctta attttattct
    49381 aaagttcctc aatagtattt acagacttag cacttatagc ttataagtct ttatattatt
    49441 aatctatttt tataggttaa ttgtacaaat catatatcac ttttacccat taaatgcatt
    49501 ataaaaaaga tggaataacg attcgttact ccaccttttt tgtaataaaa aattgtatat
    49561 ataaacacta tttactactc ttctaagtac aaacagtatc tacatactat aacctttcac
    49621 cattagattc tattacttgt ttataccagt caaaactctt tttcttaatc ctagacaagt
    49681 ctccacttcc atcatcatac ttatccacgt atataaaccc atatctcttg gccatctcac
    49741 cagtagatgc acttactaaa tctatacatc cccatggagt atatccaaat aaatcaacac
    49801 catcttctac agcttccttc atttgctcta tatgtgcttt taaatactct atacgatagt
    49861 catcatttat cactccatta gattcttttt tatcataagc tcctaaacca ttttcaacta
    49921 ccatcatagg tattctatat ctatcataaa tttcatttag tgcatatcta agcccttcag
    49981 ggtctatttg ccatccccat tcactagact taagatatgg atttttcact ccacctaata
    50041 tgttgccact tgtattatct tctttagaat cactacttac acatgtagac atataataac
    50101 taaatgtata aaaatctaca caaccttctt ttaaaatttc taaatctcct ttttccattt
    50161 ttatttcaat attattttct ccaaaatatc tattcatata agttggatat tcgccacgca
    50221 cttgtacatc tccacagaaa taattcatta ctctactatc tctttgagtt ttaactgcat
    50281 catctggatt acaagtataa ggatacattg ttgcaaatat ttgcatacat ccaacttgat
    50341 aatttgaatc tatttcatga gctaacttaa cagccttagc acttgctaca aattgatgat
    50401 gtaacccttg aaaacgaagt tgaagtatat ctacttggtt tataaaatct gtagtccctt
    50461 catttaatat tccttgtgat aagtagcctc ccataggcat tgtagctgaa tttatttcat
    50521 taaatgtcag ccaatatttt acttttcctt tatatctttt aaatattgtt tcacagtaat
    50581 tcatatacaa gtctattaca tctcttgaaa cccatccatt gtatttctct gttattgcaa
    50641 atggtatttc atagtgagat attgttatta atggttctat attatatttt aaacattcat
    50701 ctatcacatt ttcataaaat ttaagtcctg cttcatttgg ttctttctcc attccagttg
    50761 gaaatattct agtccatgct attgaaaatc taaatacctt aaatcccatt tctgcaaata
    50821 aagctatatc ttctttgtat ctgtgataaa aatctattgc ttcatgactt ggataaaatg
    50881 ttcctttttc tataactctc gttattcttt tacttacttt ttgagaacct ccagtacaca
    50941 tatctgaaca agaaggacct ttcccatcta aattccatgc tccttcacat tgatttgctg
    51001 ctgttgctcc accccataaa aaatcttttt taaatgacat acctatatcc tcctattata
    51061 attcttcacc atttgattct attacttttt tataccaggc aaaactcttc tttttactgc
    51121 gttttaaagt tccatttcca tcattatctt tatccacata tataaatcca tagcgcttct
    51181 tcatttctcc agtacttgca cttactaagt caatacatcc ccatggtgtg tacccccata
    51241 aatcaacacc atctaactca acagcatcta tcatggcttt tatatgagca cgtaaataat
    51301 caattctata atcatcattt atatttccat tttcatctgg attatctact gctcctagtc
    51361 cattttcaac tataaataat ggtttttgat aacggttata taaagagttc atagttattc
    51421 taagtcctaa tgggtctatt tgccatcccc actcacttgc atctagatat ggatttttaa
    51481 gacttgcaaa aacatttcct tctgttaaaa tatttacttt tgggtctgca cttgtaagtc
    51541 ttgatgagta atatgagaac gaaataaaat ctactgtgtt ctctttaagt atctttttat
    51601 catcttcttc catcactata tttattcctt ttctctccaa ttcttttaat gcatatgttg
    51661 gatattctcc acgagattgt acatctataa aaaagtaatt ttccctatct ttttctatag
    51721 atttccatac atctagtggg ttacaagtat taggataagt atttcctgct gctaacatac
    51781 accctatttt aaattcttga tttatgttat gtgctagttt agtaactaac gcactcgcaa
    51841 ctaattgatg atgtgcagct tggtatttta tttgttcttc attttctccc tcttcaatat
    51901 agagacctgc acctataaat ggtaaatgaa gtatcatgtt tatttcatta aatgttagcc
    51961 aatactttac cttaccttta aaccttgtaa aaataacttc acagtatttt acatataagt
    52021 ctactagctt acgatttctc catgctccat atttttttac caaatacatg ggaacatcaa
    52081 aatgacagat tgttactact ggttctattc catactttaa acactcatca aataaatctt
    52141 catagaattt tagtccctct tcatttggaa catcctcaac tcctgttgga aatatcctag
    52201 tccatgctat tgacatacga tagcatttaa atcccatctc agcaaataat tttatatctt
    52261 ctttataatt gtgatacata tctattgctt catgagctgg gtaatagtgt tcatcatcac
    52321 attcaaacat tttcattttc ccagccaaaa ctggaaatct atcttttcct tgcggtgtaa
    52381 cgtcaacatt agctagacct ctaccatctt ctttatatcc accttcacat tgatttgctg
    52441 ctgttgctcc accccataaa aaatcttttc taagtgccat aattctacct ccaataattt
    52501 atttatagta actttatttt aaaataaaaa tacatatata taattcttaa ttaatagaac
    52561 tctcttttac gctaaaacag ttatcaattt ctgactatta tttacatgct tttgtgtatt
    52621 ttctacaata tccacataat cagaataatt agtaacaact actggtgttt ctaagcaata
    52681 tccttctttt gaaatacttt ctatgtcaaa agttattaat gtctgacctt ttttaatctt
    52741 atccccttga ttaacaaaag atttgaaata tttaccttcc aactgaatag tatttaaccc
    52801 tatatgtatc aatagttcac aaccattatc agatataatt cctattgcat gttttgttgg
    52861 aaataaagtc attacagtcc catcaaatgg tgccactact tttccttctt taggtgttat
    52921 tgctacacct tttcccaata tacctgttga aaacgctgca tcttgtatct gctccaaagc
    52981 ctttacttca ccttttattg ggctatatac tacttctctt ttgttttttt ctataacgcc
    53041 ttgcccatta tcatctttag aaatttctac tatagcatca tctttccaaa aaaataatgt
    53101 taaaacaaat cctactacaa tagctactat tattgctatt atagttgcat aaacaccttt
    53161 catatctcca gttgttggat ttatataatt aggtatacca aatattccaa gtccacccat
    53221 tacataactt tttacattca ttatccccat tacagctcct gccacaccac cacctacaca
    53281 gctatatatg aatggtgttt ttcttggtaa agttattcca tatattgcag gctctgttac
    53341 accacatatc cctgaaataa ctgcaggtat acacagctct tttatcttct tatcttttaa
    53401 tttaaagtac attgctgcta ctactgctgt ttgtgcaaaa gatggtacaa atgctcccac
    53461 taatattgtg tcaaacccca tagtcataag gtttgacata gctattggta ctaatcccca
    53521 atgtagacca aatattacaa gcacttgcca caataaacca gttaatgcac cacataaaat
    53581 tggtgaaaag ttaaataaag ctaaaaatcc atttccaact atattagaag cataggtagc
    53641 tataggccct ataaccataa atcccaatgg taaagaaact aataaaactg ccattggtac
    53701 aaagaaattc tttactacat ctggtattat tttagcaaac actttctcca acttagctgc
    53761 taataatact attaaaatta ctggtactac tgttgaagtg tagttcattg ctattactgg
    53821 taatcctaag aaagttatat atactggtga ttcaaatatt gttccagtaa atagattgta
    53881 taaaggttga cctgtagatg ctagagtatc taattgaata cctgaatagc ataaaactgc
    53941 acctataagc attccaataa atggttttaa tttgaatttc ttagcagctg tatatccaag
    54001 aattattggc atgtactgga acattgcatc tccaattgca tttaacataa tatatgcacc
    54061 atcagtttct ccatacaaac ccaatgctgt aaatactgca ttaaatcctt ttatcatacc
    54121 agcagcacac agaactccta atattggttg aaaaactcct gatattatgt ctataaactt
    54181 attaagtaat ccttttggcg catctgagtc tgaattatct attgtatctc ctcctaatcc
    54241 agccaaatcg cacacaactt cataaacatc tggaacatga ttaccaataa ctacttggta
    54301 ttgacctgca cttttcatta cagtaactat accatccatt ttttttagca catcatcatt
    54361 tgctattgac tcatctttta atttaaaacg tagcctcgtt atacagtgag ataaactgct
    54421 aatattatcc tttcctccta cattcgcaat gatttctttt gatagttgtt catactttct
    54481 cattttttct cctccatttt ctttttgaaa tatgaaggtt tggccaactt aatgtcacca
    54541 cccaatatat ataaacctaa ttattaggat ttatatctga cttaatactt ttacttacca
    54601 ctctttcaac gtgaatagtt aagtataatt tttcctcatc tgttaaatcg tagttgtatt
    54661 ttttatgtat aaattcatcc aacttttcta tgaatttata agcattttta tatttaactt
    54721 ttattacatt taataaatca tcttcattat tttcatgtaa attattacta acaattcgtt
    54781 ctgcaaaata ctttaagtga gtaataaatc tatgataata aactgaatct tcatcaaata
    54841 ctattctgta atggtattta accatatttg aaatttcttg tattatttgt gtaatctcat
    54901 atatagtttt tatactttga tccatttgtg cattaactat atgcagtgct ataaatccag
    54961 cttcatcttc tgatagtcta atttcaaatt ttttttctat ataatccaat gcctctaacc
    55021 ctattttaaa ttcactctta taaaatcttt ttatatccca aagtaataca ttttttacac
    55081 ttactccttc acgagctctt tctattgcag tatacatatg gtctgttaaa gaaatataaa
    55141 tactatcatt taatcttttc cctagttttt ctttagcata taaaattatt tcatctgata
    55201 attccatata tcttatagga atatctgcta ttagttcttt aaatttatca gatatatttg
    55261 ggtctgaaag tttgaatatc ttatcaattt tatcctttga tatatgctct cctgtacgtt
    55321 ttttataagc taaacctctc cccataacta ctatttcctt atgattttca tcagttgtaa
    55381 taattacatt attattgagt attttttcaa taatcattgt gatgccccct ttattagcat
    55441 taagttaaaa aataaatctt ttttaattat ttagaatatt taaaatgtaa tttattacat
    55501 aaaaaaaaac ctatttatta cacattaaat agactcccat gagtcctgag taataaatag
    55561 gtttagcttg tatttaaaca atcactatcc gtttgtattt tattttaatt attatttatt
    55621 atatgttagc aaatttattt tttagtgtca actttttctc aatattttaa ttttaaataa
    55681 atttatataa attgaatctt caaagtacta tttttatcaa taaacaaaat aaaaaaggta
    55741 ttactttctt taagtaatac ccatttattt ttaatttaag tggtttttct taatctcatc
    55801 aagtagtcca tcttctaaat aaatttctaa tgcacatcct accattgcct ttactgcttt
    55861 gtgaagttta tcataagctt caccagaatt tgcataattt agaaattctt tttcatgaac
    55921 atataccatt ggacttatat ctaaggctat ttctgtatac atagtagggc aaacttgact
    55981 tacattacct atatctgtag aacctactgg accatcttta ccttctaata tatctgttat
    56041 acctgcctca atcaaattat tctttgttat cttttgaagt actttgttat ttattatgtt
    56101 gtcaaaagaa ttttcaaagt atctataaga aaacttagct cctgtcatta actctgcacc
    56161 ttttgcacag tttataactt ttttagttac ttcatctaaa taacttcttt cagcagctct
    56221 tacgaaaaac ttacaggctg ctttttctgg aactatattt ggagcctctc ctccatttga
    56281 aactatacca tgtattctaa catctgattt tacatgttgt cttagtgcat ttatcccagc
    56341 aaaagtaagt tgtactgcat ctagtgcatt gactcccata tgagggtgac tagctgcatg
    56401 tgctgatacc ccttcaaaac taaactctaa tgaatctatc gccaaagcct tacagtttat
    56461 attattaaaa gcttctatat gcatctgata acttacatca acatcatcaa aagctcctgc
    56521 cttaactaaa tcacattttc ctcctgttgt ttcttcagct ggtgttccta ttagtacaat
    56581 ttttccttta aaattttctt ttaacttact taatactata caagctccta gtgtagatgc
    56641 tgcaatccag ttatgtccac aagcatgagc tggttcttta ttttctccat atccaggaag
    56701 tgcatcatac tcagctaaaa atgctatggt aggtccatca ttatctccta attcagctct
    56761 aaaagcagtc tccatattac aatatggata aactgtatca aatccatatt ccttcatttt
    56821 ttctactaag tattttgatg atatgtattc ttcttcccct aattcaggat tgtcaaatat
    56881 aaaattacaa acttcttcaa tttcttttcc aagttcattc tctatctcat aagctctttt
    56941 ctttatactc tctatcataa tttacctcct actagaatcc tagatataat cctattgtca
    57001 aaaatgccat tacaagaatt gttaatacta ccattaaagg aattacgaat ctaacccact
    57061 ttgaccaagg aatattggct actgctagtg ctcccattaa aacacctgat gtaggtgtta
    57121 tcaagttagt tacacctata ccaaattgat aagctgtaac tatcatttgt cttgatacat
    57181 ctactaagtc gccaagtggt gcaagtacag gtattgtaag tgatgcaagc cctgatgctg
    57241 atggtattaa aaatgctatt aatccttcta ttaagaaagt taaatttata aatatagttt
    57301 ttggtaatcc atttaataaa tttgctgcag aatttaaaat tgtatctatt ataaatccat
    57361 tttctgctat tattacgatt ccacgtgcaa atgctataca caatcctgct gtcattaaat
    57421 ctcctgctcc acttataaat gaattaacta tatcatcttg tttaagacct gatgctatac
    57481 ctgatattat acctatagct gtaaatatca ttgcaatttc agttatatac catcccaact
    57541 ttaaaactcc atatatcata atcgccatac ctattgtgaa aattgctatt accatggctt
    57601 ctctttttgt aaattccttt atatttgtat cactattaac cattgattct tgatttagtg
    57661 atatatcgtg tactaaagat tttttagggt cttttttaac tttattggca tacatcataa
    57721 caaatcctat agatatagcc atatatatta taaacataac aactctaaac tctattccag
    57781 aacctggtgc tatttgtgat aatgcttgtg ctattcctac agagaaagga ttcgtagtag
    57841 ctgctccaaa tccagctgtt gctcctataa atactgtggc cactgctgtc aatgaatcat
    57901 atcccatact ggtcataagt ggaataaata tcatataaaa aggtagtgtc tcttcactca
    57961 ttccaaacgt cgtcccacct agtccaaata gaatcattga aatcggtatt attaaaaatt
    58021 gaaacttctt cattttattt acagtatgag ctataacagc ttctatagct cctgttttat
    58081 taactatccc aaaagctcct ccaactatta aaacaaatcc aactacttca gcagcatcta
    58141 taaacccgtt tataggtgct gtaaatacat cagtaattcc ttgtggattt gatggtgctt
    58201 tttcataagt tccagctaca acaactgacc ttccgtctat atctactctt tcaaaatttc
    58261 cacttggcac tatccaagta agaattgcca ttaacactat tagcaaaaat attattgtaa
    58321 gtgtctgtgg catagtaaac tttttctttt ttaaactgct catttttaat ctccccactt
    58381 tttaaattaa atattttatt attgcatccc tttatcctat tttgtataga tgtaaaaaag
    58441 ctcaacatgg atacatgttg agctaaatgt gctaaataca acatacctgc atcttccctt
    58501 tatcttgttt aagatagctc tccatcaaaa ttcgggataa ttttgatgac agtactgtac
    58561 ctattaaata cagtcccagc actagcttcg gacactagca cttcggcact tactcctttt
    58621 atattgcctc attggtttcc aacctcttca atattaatct tagtaaatgc aacctctacc
    58681 tcaatctgtt tgttagatga ggtaattctt attaattttt attaattcaa agtaattgta
    58741 gcataatatg tcgagattgt aaatattttc agtataataa ttcttacttt atgatttcat
    58801 tccatttttc acaaataatt ttttctccat ataatttaaa tactgtagta tattttaaat
    58861 acacacaaac tttccacaaa ttttaaatta ttataatacc tttataataa aaaataaaag
    58921 ctatgtaaac ttatcaattt acacagcttt atatacatta atctactcca agtacagctt
    58981 taagctttga tgctattgta ataactcgtg gaccaaatat aacttgaact gagtttttct
    59041 ttttaacaac tccacttgca cccaattcat ttacccagaa gccatcttca tagaatccat
    59101 caattgaatt tagctctacc ctaagtctag ttgcacagtt tgtaacattt tttatattat
    59161 tacttcctcc taaaccttct attatagaat gtacaagctt tgtatcttta tcatctactt
    59221 cctcttctac tgcattgttg tatgctgttt taccagcatc tacagattgt ctttctctat
    59281 attctttctt actataaagc ttaacttctt ctgagcttct tcctggtgtt tttatatcaa
    59341 acttcttgat tgcccatgta aatattatat aagtaactat aaaaaatatt ggtatcaaaa
    59401 ataataatgg taacgcatcc actttttgtg gttgtaataa attaggaaac atatttctaa
    59461 atgattctcc cattatagaa acattaaaca tttctgtaaa tacatacgtc aatcctgcca
    59521 gtggtacata tgctacaaaa tacaataatg gtgcaacaaa taggaatgta tactctattg
    59581 gttccgttac ccctaccaac atggctgtta ctattgctgg tatcaatata cttattgctt
    59641 ttttcttatt agcattatca gccaacttat acatagctaa agctgctgct ggtaatattg
    59701 caaaatgtat taataaacga cctgttgtga agttacgagt tatataagat agtgtatctg
    59761 ggtctcccat ttgagctaat cttatattat taacaccctc gtaaattgct ccacctactt
    59821 ccattgttcc acctactctt gtatattcta tagggaatgc aattaagtga tgtataccaa
    59881 atggtaataa tgctttatcc attgctccaa aagtaaatgt tcctaataat ccactagaac
    59941 ctatccaatt accaagttta gataatgcca accctactgg tggccatata aagtacattg
    60001 ctgtacccaa tactattgat gctataaaca tcataatcat tgaaaatctt ggacctgaga
    60061 aaaaagataa tgcactagga agttctatgt caacaaattt attatgtact gcactagcta
    60121 caaatccagc tattatagct ccaaatatac tacaatcata tgaaaagatt cctaagaaag
    60181 aaccatataa agaacttcga ctatatgctt ctgcctctcc taatccagat gccataaacg
    60241 cttctgctgt aactgtatct gaatttatac catttgctgc taacaatgta cttataactg
    60301 tgtgcattcc tataaataaa acaattcctc ctaatgcacc ccatcctttt tcctttttag
    60361 ataatccaaa ggctatacct gtaacaaaga atattggtaa gtatctcatt accataaatc
    60421 ctaaatcgtt tattacttta aagaatacat ttataaatga cccttctttt attagtccgc
    60481 taagccctaa tgcagaaacg ttttccatat ttgcaaaagc tgaccctaat cctataaata
    60541 ctcctgctac aacaagtaat ataacaggta tcatcattgc tccagaaagt acttgtaatt
    60601 tatccttcat agtattattt cctccatttt tattgaaatg ccaggctata taatgcatat
    60661 atatatactt ctattgcttt gtataatgta tctatactta tattttcatt tgcctgatgt
    60721 gaatttggat tatccccctc tattgtgggt ccaaatgcaa ctccattttt taacactctt
    60781 gcataagatg cagctccttt tgtaaaaagt tcgacttctt ttccaaaacc ttgtttatat
    60841 gcatttgata atttatttat taattcacta tctggtttta catacagtaa ttttaaatcc
    60901 ttatatatat caactattaa attttctttt tttccaattt cattaattct attagttata
    60961 aactcccaac ttataccctt aggatatcta aagtctattt tcatattcaa ttcatttttc
    61021 tctaagaaga tactcattat acatattgta gaaactccca tatctacatc ttctttatac
    61081 atccctaatt tttttccatg attatcatct gtaaagtatg aatttaaaat atcccttatt
    61141 ataacgcctt tatcatttag ttttttaatt ttctctaaat cttttgctaa actaaatgca
    61201 cagttatatg acctgtgtgg atttctcgat aaagcccttt ctcctgtata tcttactaat
    61261 actttacctt catctaattc ttctatctct gtataataag ctaatgattc tactaaatct
    61321 ttcttatcat tagttttaaa aacagcctca attttgtcac atacaaatcc atcttctttg
    61381 tttgacttta tatcaactaa attgtgattt ctaaatttat ctttatctat tttctttctt
    61441 agattgaagt ataaaatacc tttttctcca tttactattg ggaagtttcc atctggagaa
    61501 aatgcaaatt ttggctgttc attgacacta aagtaatgct ccatacattc ccaagtcgtc
    61561 tcttcagctc cacccactat caatctaatc tttctcttcg gcttttcatt aagttctctt
    61621 aagaaaagta atgcatataa aatccctatc aaaggtcctt tgttgtcatt aactcctcta
    61681 ccataaagat agttatcttt ttgacatact ttaaaaggtt tactcttcca aagttcttta
    61741 ttatatattg gcaccacatc tatatgatta agcaccccta ctacttcttc tccttctcca
    61801 tattctatat gaattgcata tccatcaaag tcttttacaa caaaatcttt gtcttttgct
    61861 atttgaataa gcttatcgaa ggcatttctt atttctatcc caaatggttg atttatatct
    61921 gttgtacttt catctctaat tgaaggtatt tcaactagac ttctaatatc atttatgata
    61981 ttttcttgat tggattttaa ataatcaata attagttctt tgtttactat catcttaatt
    62041 atttatcacc cctttatatt aattttacaa agcatttcag ttcatatcta cattataaat
    62101 tttgaatatt attttttcaa tatttcaaat acttctctaa gttaccataa tgatacggta
    62161 aacaatcaac agattatgac aaaatcatac tatacctatt actaatattt taacttatct
    62221 attacgtctt ttttaatgta agtttattcc aaaaaataca taaaaaaaga tgtagcctta
    62281 gctacatctt cttatatcta ttcaacttcc attattatgc tattaatatc tacatttcca
    62341 ggttctatcc tatcttttac tgttctctta acagaatcag catcatccat tataactaca
    62401 ggagttatta tatttaaatc atttgacgtt ataaaatcta aatctgcttc cattattaaa
    62461 tctttagctt ttaccttatc tcctgccttt accttaacat caaacggctt tccttctagt
    62521 gacattgtct ctaaacctac atgtattaat atatttgtat tatcattact cttaatcatt
    62581 attgcatgct ttgtatcaaa tacattgact atttcaccat caacaggaga atataactta
    62641 ccttcacttg gtattattgc tactccatct cccatcatct tttgggcaaa aactgcatct
    62701 ggaacatctt ctaagttcac taaatttccc ttaaaagggc tatatatttt ctcaatctta
    62761 ttttcttttt tcttaaatat attaaacata acacactctc cacattataa aattttatga
    62821 taatattttt cttaaagctt ttgctacacc atcatttaca ttagtatcag ttacaaaatc
    62881 tgccatagat tttacttcat ctgtagcatt tcccatagca ataccaagac ctgcaaattt
    62941 aatcatggat atatcatttt cattatcacc tatacaaata acttctttgc tatctatacc
    63001 taatatatca caaaattttt taactgcaat accttttgaa gtacctttac tcataacttc
    63061 aaagtttatc tttccagaac ttactacttc taaatcttct tgttttttaa attcttcctt
    63121 tgcttctctc aaagaatcta cattttctga gaaaagtata cattttaaaa tttcattatc
    63181 agctttttct aataaagttt cacaacttgc attttctatt atttcaattc tgtcttcttc
    63241 tttattttta ctatttatta atctatatgg atgttcttca cctatttcat tgttggatat
    63301 aactatttta cagcctttaa aatgtttgtt taaattatac ttttcaccta ttgtatagat
    63361 tttctttaga gcttctttgc tcaaaacctt cttatactca tatcccagta atttaaaata
    63421 tgtcccatta gttgatataa tatatatatc atcccctaaa acacttgtaa aatatttcat
    63481 agcattataa ggtcttcccg taactaaggc taccttgata cccttttcca tgacttcttt
    63541 tagtgcttta atattttctt ctgatacttc aaatcctttc cccatcaaag taccatccat
    63601 atcagtacaa attaatttat atcgcatttg tatcacatcc tagtaagatt tttctaatat
    63661 ttcaacaagg tcttctttct ttccaaaagg taaaaatcct aaaactggac agcttgttat
    63721 ttgttctaat tgttcctttg ttacattaaa ctcagacatt tttgtcttaa gtcccactct
    63781 ctttaaaaaa ctttctattt catttcctaa agttttagct gcttcatcat cttccacttt
    63841 ttctaattct tggtttagca ttctcgctac tttggcaaat ttctctatat tatccttata
    63901 gaacttttct gcaaattttg ggaacactat agccaaagcc tcaccatgag gaatatgtgt
    63961 aactcctcct attatttcac ttagtggatg tggtgctgct gctccagcat ttgccagtga
    64021 acttcctcct aatgtatctg ctactgctaa ctttgttctg tattctatat tatttttatc
    64081 ttccattact ttaggtagat agttaatgac taattccata gatttttcag acattatctc
    64141 tgtaaaatca ttagcatttg gatttatata actttcaaat gcatgggtaa aagcatcaaa
    64201 tgctgttgat gcagtaattc tctctggcag agttaacata agctctgggt caactataca
    64261 ttcttttgag aaacaatttt gatgaaatat tgtatttttc tcgcttcctc tcgatattac
    64321 tgctgcttgt gtaacttggc ttcctgttcc agaagtagtt gatacagcta ttaatggtaa
    64381 ttctttttca cttaccttat cgtatatttt aaatggattt gtatatgtac taaacatata
    64441 atcccaatct attttttcta aaccaaaagt caatgctaat acttttgcag tatctatact
    64501 tgaacctcct ccaacagcta aaacaacgtc tacagattct tttttagcta attcaaaccc
    64561 ttcgtttatt aattctacag ttgggtttgg ctctactcta tcaaagtgta caacttctat
    64621 acctttttct attaaattca atgtaactct tttatataac ttgtctaatg gctctacatt
    64681 tggagtagta actaacaaac atttctttcc atattttcct attattctag gtaattcact
    64741 taaagttcct tgtccaaaat ttattcttga tggttgataa aagttaatca tatttttcct
    64801 cccttaaatt attgccttca tattcaaatt atacatatca taggattttt aaggcaatac
    64861 cttcactgtt aactcttatt tactaaagct tatggcaatt attagacaat caatgtttta
    64921 ttattaccat atcattatgt aaattttatt aagtacccat gttataattt tataatcaat
    64981 cttattgtat aattgactta ataataaaaa tagagtatat acactttttt atgacaaata
    65041 gcttaaagga gaattaaatt atgaaatatt tgatagacag tgctaatctt gatgaaattc
    65101 aaaagtgttt aaagtatgga tttaagggaa taacagctaa tccatctatg tatcttaaag
    65161 agaaagtaaa cttccatgaa tttattgaga catgtgctaa tttaaatcca gaaatattaa
    65221 ctgctgaagt cattggaaat aatttatacg aattaattaa ttcatgtgat aagatattaa
    65281 atataaataa aaacactata ataaaaataa atttctctga agttggctta gaacttataa
    65341 acatattaaa tagtaaaggt gtaaaaactg catgtacctt gattttcaat gtaaatcaag
    65401 catcattagc tataaatgca ggtgctgatt acctattccc ttttataggt agaaatgatg
    65461 aaaatgggaa tgatggtata aaaaacctta cagaaatatg taaccttata tcaataaatg
    65521 gatataatac taaagttgtt gcagcttcta ttaaaaatgt attccactta actcaagcat
    65581 ctctttgtgg atgtgattat gcagctgtaa catatgattt atttaaaaaa gcttctttta
    65641 accagcttac aatagatggt gccaatactt ttgaaagaga ttggtccaag gttaataaat
    65701 agtaactaag atttaggagg gtttgcattg aacaaaagat tattagatat tgctgaatta
    65761 ttactcaaca gctcagacta tattactgta gatactattg ccgaagaatt aaaagttagt
    65821 aataaaacta ttcgtaatga ccttgttatt ttagatgaat ggcttttaga gtttaattta
    65881 tccctagata aaaaaactgg ttcaggggtt attattctag gtaatgaaga tataaaactt
    65941 aaagttatta gagatatcaa tgataaatca aattgtatat atgcatactc tcctgaagat
    66001 agaaaaaggt atattctaag taaattattt attaaaaatt ctaagtttag gattcgtgat
    66061 atttgcaatg aactacatgt tagtagagct actgtgcata aagacttggt agttattcaa
    66121 aattttttgc aaaactttaa agtgactcta gttagaaaaa ccaataatgg agtatattta
    66181 gagggaaaag agaaagatat tcgaaaagca atttttgaac ttgctactgt taataaaagt
    66241 tatactgaac ttaaagaaat attattctcg aaatctaatg aatgcaaaga taccccatca
    66301 acaaaaatat ttaaggagct atttaactat gattttgaga aattatctca aatcactcta
    66361 cacaccttac aagtagaaaa aagtaacctt agtgatgagt attatataaa cttcttaata
    66421 catatagcta tatgtattaa aagaatttca attaaaagat atattaactt atctgaaggt
    66481 ttttttagag aattattaga acatgaatac tttgaaaaat caaaaatttt atgtgctaac
    66541 ctaagtgaag cttttgacgt agaatttatt gatgaagagg tctgttatat ccttcttcat
    66601 atagaaggat tacttaaatc aataaagaaa actactactc ttgaacctct taaaaatgaa
    66661 gaaattacag agtataatct atctgaagca atagctgttc attggggaag tatactaaat
    66721 ttaaacttaa aggacgattt aatattaata aagtctcttg ctaatcacct taaatcagtt
    66781 ctacacagaa taaactatgg gtttactatt acaaatccca tattaaatga tataaagaga
    66841 acatttcctt atacttataa ggctgctaaa gagtctaata ctgttatcaa acagttgatg
    66901 aattatgaag tcaatgatga cgaaatagga tatcttgctt tatatttaat atcagcaata
    66961 gatagaagca aatctcccct aaatactatt ttaatatgtc actgtagtga tggtgtatct
    67021 aatttattag tccaaaagtt aagttttgaa tttaatcaga ttaatatagt taaatctata
    67081 cctctatctt ctatttcatt tacgaacttt gatgatgtgg acttaatact aaccactgct
    67141 cctgtagact ttgaacataa tgctgaactg ataaatataa atgctctttt aagtaagaat
    67201 gatatttcta gacttagtac aattataaaa aaactatatt ctgaaaaaaa taaaatatta
    67261 tcttataaat aaacaaaaat aggcttgtct caacatagag ataaacctat ttttcattgt
    67321 ccaaatgata aaaatatatt tgatttcata gaaaaagtgc ctcatgaact aaaaaattca
    67381 ttttgagaca ccttcatttt ttatatttaa tctttttatt taagctttta tattttttaa
    67441 acttttatat ttaaagtaaa ttttattatt tttattgagc acaagcatta tttatattat
    67501 taatttcttc agtattctta ccaaactttg tagttacaaa tcctattgtt tttattatga
    67561 gtaataagga tatcaatgaa tatatagata tcataaaatt cttacttatt attccagacg
    67621 ctactattat aaatccatct agcataaata atataactgg caacttcaga aacttaatta
    67681 ccttattaat aagcattgca actaaatcag ttccaccagt actacagccc cttattattg
    67741 ctatccctat agcagttcct accaataacc cacctatcac tcctgctact aatagattgt
    67801 cagttatgtg agtttgtggt attactttta agaataatgg gcccataaga gttatatata
    67861 atgtttttat tccaaaacct ttccctaaaa ataataatcc cattactaaa agtggcatag
    67921 atattcctaa tgatatattc tcaacaggca ttgataagaa tgtgctaagt acgactgata
    67981 atcctgttat acctcctgga gctaatgtat gttctgcaaa gaaaaaatta actgctaatg
    68041 ccattaaaaa tgctgatatt gttatcagta aataatcttt tatcatttct tttttcattt
    68101 aataacctcc tatgattata ttgtttcagt ataaaaaatc aattaaatta gtctagcttc
    68161 ctgtagtttt tcataaaatt aatgcttcct taaaacatta agaaagcatc tcttttatca
    68221 tagtttatta tcttattcta aaccaaactt tgtagctgct tcatctatag cttttctaca
    68281 attcttaaca cctgctgtta taccatcttt atccttgaat attgctgaag ataatattac
    68341 aacgtctggg ttacattcta ttaatggctc catattgtct gctcttagtc caccatctat
    68401 tgctaattca cacttaggat tcttttcgtc tatcattttt ctagctcttt ttactaaatc
    68461 aacagcactc tttctccatc cccagttatc ttttccatca gtttcatcaa caccatgaac
    68521 gactatttgc aatctatcta tatcatatat agcttcatca acaaaacata atggtgtgta
    68581 acatccaatt gttaatccta ctttcatacc aaattctcta cagtaattta ttatgtatgc
    68641 aagaggagca cctaaaaaat gttctgctgg tattatcaac aagttacatc ctgctgctgc
    68701 tattttttct ataaataatc tatcacaatc tcttgtgtat atatggcatt ctattggttt
    68761 atctgtcact ggtcttattg cttctattat ttgatgacca cccataagtt gcatattttt
    68821 taagtcatgc atatctgctg catctgaatg tacgtagtct acacctgcat ccattgcttc
    68881 tttaactacg gctgatatat gaccgtaatt tacatgtgct aatcctgctg ctatttttat
    68941 gtgtttcata tttgtatacc tccatatttt actttaatta gtattaaata ttagtttttg
    69001 atattttttt aatatttttt aatccagtat tacttttatt tagcattact tttattgctg
    69061 tatatatttc ttttatgaac ttattctgct tttgttaact gtgaacttat tttgctttgt
    69121 aaattaatat tacactgata tatttttttt cacaacactt tgactactcc ttaaaagtac
    69181 ctttatattg tggtaaatta tagacattaa ttgtatataa aaagttagtt ttttaccaca
    69241 ttcttatggc atttattgtc tatactaact caattgatat agctagttca tttaatatat
    69301 aatcagacta taaatataaa tttactcgat aaaaagtacc attcataaca atattaatga
    69361 aagggagatg taaaatgaga aactgtatta ttgcccaatc tggaggccct acagctgtta
    69421 tcaatgcttc tgcagttggt atttatgaaa aaaatttaga aactaaatac tttgataaag
    69481 tatattttgg tatcaatggg attgaaggta tattaaacaa aaatataata gattcagact
    69541 ccttaaacaa agataatgta ttaaatttaa agcaaacacc ttcatctggt cttggttctt
    69601 gtagatacaa actggctgat tataaaataa attcttatga ttatgataga gttataaata
    69661 tactagatga atatgaaata gatacattat tttatatagg cggaaatgat tctatggaca
    69721 cagtgatgaa attttctcaa tactgcaaaa acactacttg taaaataaaa tttataggta
    69781 ttcctaaaac aatagataat gatttattag gaactgacaa ctgtcctgga tttggttctg
    69841 ctgcaaagct tatttctaca ctaataatgg aaacatactt agatttttct atatacaaaa
    69901 ataatggtat atttatagta gaaactatgg gtagagatac tggatggtta gcagcttctt
    69961 cttcactagc tagacttaat gatactccac ttgttgattt aatatacctt ccagaaattg
    70021 atttctatga agataagttt atagaagatg ttagaaaaat atttaaagaa aaaaataagg
    70081 cttatatagt tgcatcagaa ggtataaggg ataaatacgg caactttata tgcgaacaaa
    70141 aaacttctgc tcatgataaa tttggtcatt cccaattagg tggagtaagc aactatctaa
    70201 agaatcttat agtatcatca tcaattacaa caagagtaaa aaccttagaa cttggtgtaa
    70261 tacaaagatg tgctatgcat ttagcttcag atactgattt gaaacaagca gctgaagttg
    70321 gtgcatatgg gatagaacta tctaaaaaag gatatacagg agttatggct atcattaata
    70381 gaacatgcaa taatccttat aagtatgaag ttagtcatac tgatatttct aaaatagcta
    70441 acaagacaaa atattttcct aaagagtgga taaatgaaga atgtaattat ataactgatg
    70501 aagccctaaa ttatctatat ccacttattc aaggtaaacc agatatcaaa tataaaaatg
    70561 gactacctta tttttcaatt atttactagg aaattattat aatttgatat acactcaatt
    70621 ctatcgctct taaataccta agtggctgtt aaataaaatt ataaattgtt atatgacagt
    70681 cacttaatcg tttaattatt taactgttta gttttttaat tttgtttaat actaatacaa
    70741 tttgttgtaa taatatctca cacccatttt cattttggat agattataat tagttagaca
    70801 ataaatattt tgtcatgata ttattatata tagtgataca aatagaaata cttttatcat
    70861 ggctagaaaa acaaagatta cacaaaggat ttaaaagtca aacaacttca attaaaataa
    70921 atggttaaaa taacttctaa atcaactaca taaacaataa aagaacataa agtagttaaa
    70981 tctacaatta taaaattagt aagactataa taacttaaat aaaattaaag ttaacaaaga
    71041 ctattaaagt tagcaaatat tcctctacct aaataattgc tttttataaa gagttattta
    71101 ttttaattga atttaattga aatacgatt
//
LOCUS       DACXMZ010000002        69658 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.002, whole genome shotgun sequence.
ACCESSION   DACXMZ010000002 DACXMZ010000000
VERSION     DACXMZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 69658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69658
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.002"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            172..1167
                     /locus_tag="JD016_00290"
     CDS             172..1167
                     /locus_tag="JD016_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAU4998061.1"
                     /translation="MSFLQAKNVGKSYGQVKVLKDISIDIEKGEFICLLSPSGCGKST
                     LLRIIAGLEDKHDGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSDIDKKVRDILEVVELSGYDKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIKSLHRKLGITTIMVTHDQEEALCLADKMVVMNNGEVIQIGTP
                     KEVYKSPKTPFVADFIGTINFIDDDVNMIAIRPEDIKVEFNRDFKEIDVKVGEILDIE
                     FRGFNYRITVEYCSKQIKLDVVSKVVEQMKLCVGSKINFKIPKERTIQYKSEGCA"
     gene            1185..2873
                     /locus_tag="JD016_00295"
     CDS             1185..2873
                     /locus_tag="JD016_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HAU4998062.1"
                     /translation="MTTIKNKRRISDSKIQKCFLVFLVIALLVFILFPLISLLKNAFM
                     DTSGSFVGISNFSKYIENPTLIASFKNSIFVSTISSLISLCLAFIYSYAITRTNIRFK
                     NFFKTMGIFPLFAPAMLYGISLIYLFGNKGIFTIIGIEVPLYGPLGIIICQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFFKITIPGIKYSLVSSFFVSFILAFTDF
                     GAAKIVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTVDKIASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIACSFITLLIFILIFVSVIGSVIKLWPYDLSFTMEHY
                     TFKGISGNGIKTYLSSLKISILTAIFGTIFVFFSAYMIEKVDKFPRLRQISYFLSMIP
                     MALPGLALGISYITFFNSASNPLNFLYGSTTIMVIANIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSINIPFYKVFFYVTLPMSITSILEVAVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLEEIGYIGPSAAMAVLVFLTNIIVKLLYELTTKKLKTKTQKWQQKEVA"
     gene            2885..3952
                     /locus_tag="JD016_00300"
     CDS             2885..3952
                     /locus_tag="JD016_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAU4998063.1"
                     /translation="MKFRKLVSLGILSTLLLSVITGCNNASSGSNLKSNNKIKGELTV
                     YTAIEEDSIDTYLTTFKEKYPDIKLNVLRLSVGDITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            4144..4674
                     /gene="rnmV"
                     /locus_tag="JD016_00305"
     CDS             4144..4674
                     /gene="rnmV"
                     /locus_tag="JD016_00305"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAU4998064.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            4845..5714
                     /gene="rsmA"
                     /locus_tag="JD016_00310"
     CDS             4845..5714
                     /gene="rsmA"
                     /locus_tag="JD016_00310"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAU4998065.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEIGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            5780..7042
                     /locus_tag="JD016_00315"
     CDS             5780..7042
                     /locus_tag="JD016_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998066.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEDNEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDSEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            7193..8308
                     /locus_tag="JD016_00320"
     CDS             7193..8308
                     /locus_tag="JD016_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAU4998067.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYEKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            8494..9390
                     /locus_tag="JD016_00325"
     CDS             8494..9390
                     /locus_tag="JD016_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAU4998068.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            9460..10257
                     /locus_tag="JD016_00330"
     CDS             9460..10257
                     /locus_tag="JD016_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998069.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(10312..11664)
                     /locus_tag="JD016_00335"
     CDS             complement(10312..11664)
                     /locus_tag="JD016_00335"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAU4998070.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            11883..12761
                     /gene="purR"
                     /locus_tag="JD016_00340"
     CDS             11883..12761
                     /gene="purR"
                     /locus_tag="JD016_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAU4998071.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            12917..13195
                     /locus_tag="JD016_00345"
     CDS             12917..13195
                     /locus_tag="JD016_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SpoVG"
                     /protein_id="HAU4998072.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            13408..14787
                     /gene="glmU"
                     /locus_tag="JD016_00350"
     CDS             13408..14787
                     /gene="glmU"
                     /locus_tag="JD016_00350"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAU4998073.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITYDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            14882..15832
                     /locus_tag="JD016_00355"
     CDS             14882..15832
                     /locus_tag="JD016_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAU4998074.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      16671..16756
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            16918..17433
                     /locus_tag="JD016_00360"
     CDS             16918..17433
                     /locus_tag="JD016_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAU4998075.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGEYKLIKVG
                     SKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTNAALSAKLTSTTKVNNEALYIVL
                     IDNIVMDQQGA"
     gene            17562..19238
                     /locus_tag="JD016_00365"
     CDS             17562..19238
                     /locus_tag="JD016_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HAU4998076.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            19250..20458
                     /locus_tag="JD016_00370"
     CDS             19250..20458
                     /locus_tag="JD016_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HAU4998077.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            20473..22185
                     /locus_tag="JD016_00375"
     CDS             20473..22185
                     /locus_tag="JD016_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998078.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDIQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            22178..23086
                     /gene="pilO"
                     /locus_tag="JD016_00380"
     CDS             22178..23086
                     /gene="pilO"
                     /locus_tag="JD016_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HAU4998079.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            23110..23679
                     /locus_tag="JD016_00385"
     CDS             23110..23679
                     /locus_tag="JD016_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAU4998080.1"
                     /translation="MMSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKK
                     QQIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKK
                     NTEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSI
                     YYNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            23716..24243
                     /locus_tag="JD016_00390"
     CDS             23716..24243
                     /locus_tag="JD016_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAU4998081.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            24264..25802
                     /locus_tag="JD016_00395"
     CDS             24264..25802
                     /locus_tag="JD016_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998082.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPDQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            25902..26984
                     /locus_tag="JD016_00400"
     CDS             25902..26984
                     /locus_tag="JD016_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAU4998083.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            26965..27747
                     /locus_tag="JD016_00405"
     CDS             26965..27747
                     /locus_tag="JD016_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAU4998084.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKRKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            27837..28499
                     /locus_tag="JD016_00410"
     CDS             27837..28499
                     /locus_tag="JD016_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAU4998085.1"
                     /translation="MLQTMEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIIT
                     VVLSVLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLV
                     FFNIVQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIV
                     LSFILAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYK
                     FI"
     gene            28513..29073
                     /locus_tag="JD016_00415"
     CDS             28513..29073
                     /locus_tag="JD016_00415"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAU4998086.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            29090..32476
                     /gene="mfd"
                     /locus_tag="JD016_00420"
     CDS             29090..32476
                     /gene="mfd"
                     /locus_tag="JD016_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAU4998087.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            32507..33502
                     /locus_tag="JD016_00425"
     CDS             32507..33502
                     /locus_tag="JD016_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAU4998088.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            33626..34165
                     /gene="spoVT"
                     /locus_tag="JD016_00430"
     CDS             33626..34165
                     /gene="spoVT"
                     /locus_tag="JD016_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HAU4998089.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            34285..35898
                     /locus_tag="JD016_00435"
     CDS             34285..35898
                     /locus_tag="JD016_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAU4998090.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            36005..37453
                     /gene="mazG"
                     /locus_tag="JD016_00440"
     CDS             36005..37453
                     /gene="mazG"
                     /locus_tag="JD016_00440"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAU4998091.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            37542..37820
                     /locus_tag="JD016_00445"
     CDS             37542..37820
                     /locus_tag="JD016_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAU4998092.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            37892..38134
                     /locus_tag="JD016_00450"
     CDS             37892..38134
                     /locus_tag="JD016_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAU4998093.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            38196..38450
                     /locus_tag="JD016_00455"
     CDS             38196..38450
                     /locus_tag="JD016_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HAU4998094.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            38451..38984
                     /locus_tag="JD016_00460"
     CDS             38451..38984
                     /locus_tag="JD016_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998095.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            38990..39274
                     /locus_tag="JD016_00465"
     CDS             38990..39274
                     /locus_tag="JD016_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAU4998096.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            39387..40307
                     /locus_tag="JD016_00470"
     CDS             39387..40307
                     /locus_tag="JD016_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HAU4998097.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            40397..42769
                     /gene="spoIIE"
                     /locus_tag="JD016_00475"
     CDS             40397..42769
                     /gene="spoIIE"
                     /locus_tag="JD016_00475"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HAU4998098.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICVSKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            43240..45012
                     /gene="pepF"
                     /locus_tag="JD016_00480"
     CDS             43240..45012
                     /gene="pepF"
                     /locus_tag="JD016_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAU4998099.1"
                     /translation="MNNNDTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDK
                     DTKELKNYIGKVTKSPTHLSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMND
                     SMSKSYGNYMGICSDLELEILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDD
                     KSEDILSKVSSISSLPSQVYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELI
                     TYNDNINTIAGLITGQVKKNIFYSQERGYESSLEMYLEADNVDEKVYNNLIRTVNSNM
                     DSLHKYIDLRKKVLKLDKVHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYND
                     VVYKAFNEKWIDVYSHENKVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVY
                     EYLSSKNQNYFNSNPSIFTHEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKD
                     TLYTQTMYAEFEKTIHELVEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWAR
                     IPHFYNSFYVYKYATGCSAGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINL
                     TNTKPIENTIKKFDSLVAELEKLL"
     gene            45126..46091
                     /gene="galU"
                     /locus_tag="JD016_00485"
     CDS             45126..46091
                     /gene="galU"
                     /locus_tag="JD016_00485"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAU4998100.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            46606..48195
                     /locus_tag="JD016_00490"
     CDS             46606..48195
                     /locus_tag="JD016_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAU4998101.1"
                     /translation="MILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNELIEIIN
                     SKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKEIDTQN
                     ISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIVDEFNT
                     SKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGITRHPK
                     SGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRIIDLIS
                     PIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDMKESIE
                     GDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLTISPTG
                     RTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEFKGTGN
                     MELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVMEITDK
                     VIELIKRTKNNKEFVKSIKNL"
     gene            48348..48548
                     /gene="rpmE"
                     /locus_tag="JD016_00495"
     CDS             48348..48548
                     /gene="rpmE"
                     /locus_tag="JD016_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAU4998102.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            48693..49586
                     /locus_tag="JD016_00500"
     CDS             48693..49586
                     /locus_tag="JD016_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HAU4998103.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            49604..50452
                     /gene="prmC"
                     /locus_tag="JD016_00505"
     CDS             49604..50452
                     /gene="prmC"
                     /locus_tag="JD016_00505"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAU4998104.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            50496..51560
                     /gene="prfA"
                     /locus_tag="JD016_00510"
     CDS             50496..51560
                     /gene="prfA"
                     /locus_tag="JD016_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAU4998105.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            51689..52369
                     /locus_tag="JD016_00515"
     CDS             51689..52369
                     /locus_tag="JD016_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAU4998106.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            52398..53438
                     /locus_tag="JD016_00520"
     CDS             52398..53438
                     /locus_tag="JD016_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAU4998107.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            53455..53904
                     /locus_tag="JD016_00525"
     CDS             53455..53904
                     /locus_tag="JD016_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HAU4998108.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            53921..54373
                     /gene="rpiB"
                     /locus_tag="JD016_00530"
     CDS             53921..54373
                     /gene="rpiB"
                     /locus_tag="JD016_00530"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HAU4998109.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            54409..55038
                     /locus_tag="JD016_00535"
     CDS             54409..55038
                     /locus_tag="JD016_00535"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAU4998110.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            55339..56154
                     /locus_tag="JD016_00540"
     CDS             55339..56154
                     /locus_tag="JD016_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HAU4998111.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            complement(56151..56408)
                     /locus_tag="JD016_00545"
     CDS             complement(56151..56408)
                     /locus_tag="JD016_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998112.1"
                     /translation="MKYSSHDGLIIYFSPLCLCTGIIYLLDFNLNKYLFLYYIHMLIE
                     CPIKKEHFYHLNFKYPSYALFNLLNLLYHLNKILIALSNIN"
     gene            56380..56817
                     /locus_tag="JD016_00550"
     CDS             56380..56817
                     /locus_tag="JD016_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HAU4998113.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            57002..57229
                     /locus_tag="JD016_00555"
     CDS             57002..57229
                     /locus_tag="JD016_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HAU4998114.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            57234..57620
                     /locus_tag="JD016_00560"
     CDS             57234..57620
                     /locus_tag="JD016_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HAU4998115.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            57623..58327
                     /gene="atpB"
                     /locus_tag="JD016_00565"
     CDS             57623..58327
                     /gene="atpB"
                     /locus_tag="JD016_00565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAU4998116.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            58396..58656
                     /gene="atpE"
                     /locus_tag="JD016_00570"
     CDS             58396..58656
                     /gene="atpE"
                     /locus_tag="JD016_00570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAU4998117.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            58779..59276
                     /gene="atpF"
                     /locus_tag="JD016_00575"
     CDS             58779..59276
                     /gene="atpF"
                     /locus_tag="JD016_00575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAU4998118.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            59273..59818
                     /locus_tag="JD016_00580"
     CDS             59273..59818
                     /locus_tag="JD016_00580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAU4998119.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            59835..61337
                     /locus_tag="JD016_00585"
     CDS             59835..61337
                     /locus_tag="JD016_00585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAU4998120.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            61365..62243
                     /gene="atpG"
                     /locus_tag="JD016_00590"
     CDS             61365..62243
                     /gene="atpG"
                     /locus_tag="JD016_00590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HAU4998121.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            62256..63650
                     /gene="atpD"
                     /locus_tag="JD016_00595"
     CDS             62256..63650
                     /gene="atpD"
                     /locus_tag="JD016_00595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAU4998122.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            63653..63913
                     /gene="atpC"
                     /locus_tag="JD016_00600"
     CDS             63653..63913
                     /gene="atpC"
                     /locus_tag="JD016_00600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HAU4998123.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            64375..64755
                     /locus_tag="JD016_00605"
     CDS             64375..64755
                     /locus_tag="JD016_00605"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAU4998124.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            64770..65231
                     /locus_tag="JD016_00610"
     CDS             64770..65231
                     /locus_tag="JD016_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAU4998125.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            65252..65842
                     /locus_tag="JD016_00615"
     CDS             65252..65842
                     /locus_tag="JD016_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998126.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            65857..67014
                     /gene="alr"
                     /locus_tag="JD016_00620"
     CDS             65857..67014
                     /gene="alr"
                     /locus_tag="JD016_00620"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAU4998127.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            67047..67334
                     /locus_tag="JD016_00625"
     CDS             67047..67334
                     /locus_tag="JD016_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HAU4998128.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            67324..67686
                     /locus_tag="JD016_00630"
     CDS             67324..67686
                     /locus_tag="JD016_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAU4998129.1"
                     /translation="MSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQNDVGNKYSP
                     TVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREKIGCLDEN
                     MMIKVDDGLQISLGLFTF"
     gene            67857..68684
                     /locus_tag="JD016_00635"
     CDS             67857..68684
                     /locus_tag="JD016_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAU4998130.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            68677..69618
                     /locus_tag="JD016_00640"
     CDS             68677..69618
                     /locus_tag="JD016_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HAU4998131.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
BASE COUNT        28045 a         7441 c        12963 g        21209 t
ORIGIN      
        1 tattaataag atatgtataa attttaattt gggaacttgt gttatatatc atgaaaaaag
       61 agaaaaaata aaaggtaaat tgtttcttga taacttgaaa ttggaagtga gaatatttaa
      121 ctagagaaga attatttaag tttaagtcag ctttaggagg aggaattata aatgtctttt
      181 ttacaagcaa aaaatgtagg taaaagttat ggacaagtta aggtgctaaa agacatatct
      241 attgatattg aaaaagggga gtttatttgc ttattaagtc caagtggttg tggtaaaagt
      301 acattgctta gaataattgc aggacttgag gataaacatg atggaaaaat aataataaat
      361 gataaggata tgactaactc accacctgaa agtagaaact ttggtattgt tttccaatca
      421 tatgcactat ttccaaatat gaatgtatat aaaaatatag cctttggatt agaaaataaa
      481 aatataagta aatctgatat agataagaag gttagagata ttttagaagt tgtagaatta
      541 agtggatatg ataaaaaata tccatcacaa ttatcaggtg gacaacaaca aagggttgca
      601 ctagctcgtg cgattgcttt agagccagac tttttgcttc ttgatgaacc tttatctgca
      661 ttagatgcaa aagtaagact taaacttagg gaacagatta aaagtttaca tagaaaacta
      721 ggcatcacaa ctataatggt aacacatgac caagaggaag ctctttgttt agctgataaa
      781 atggttgtta tgaataatgg agaggtaata cagattggaa ctcctaaaga agtctataaa
      841 agtccaaaaa caccttttgt agctgatttt ataggaacaa taaattttat agatgatgat
      901 gtgaatatga tagcaattag accagaagat ataaaggtag aatttaatag agactttaaa
      961 gaaattgatg taaaagtagg agagattttg gatatagaat ttagaggatt caactataga
     1021 attacagttg aatattgttc taagcaaata aagttggacg tagtttcaaa ggtagtggaa
     1081 caaatgaaac tatgtgtagg ttctaaaata aacttcaaga tacctaaaga aagaaccata
     1141 caatataaat cagagggatg tgcttaatag ctagaggtgg ataaatgaca acaataaaaa
     1201 ataaaagaag aataagtgat agtaagatac aaaaatgttt tttagttttt ttagtgattg
     1261 cattattagt atttatatta tttccattaa tatcactatt aaaaaatgca tttatggata
     1321 caagtggaag ttttgtagga atttcaaact ttagtaaata tattgaaaat cccactctta
     1381 tagcatcatt taaaaactca atttttgtat ctacaatttc atccttaata tcactatgtt
     1441 tagcgtttat atattcatat gcaataacaa gaacgaatat aaggtttaaa aactttttta
     1501 aaactatggg catatttccg cttttcgcac cagctatgct atatggtatt tcacttatat
     1561 acctttttgg aaacaaggga atctttacaa ttataggcat agaagtacca ctatatggtc
     1621 cattaggaat aattatttgt caaatactgt ttacatttcc acaggccttt ttaatattat
     1681 ctgtagcact ttcgatggct gattatagac tttatgaagc agcagatagc ttaggggcta
     1741 gtgaatttaa gaagttcttc aaaataacaa ttcctggtat aaagtattca ttagttagtt
     1801 cgttctttgt atcttttata cttgcattta cagattttgg agcagcaaag atagttggag
     1861 gaaactacaa tgtattggct actgacatat ataaacaagt ggtaggtcaa tttaacatac
     1921 caatgggagc tactgtctct atggtaatgt taattcctgt actaatagca tttacagtag
     1981 ataagatagc aagtaaaaag caagggatga ctataactgc aaagtctatg ccatataaga
     2041 ttaaggaaaa taagtttaga gatagagtat ttttaatagc atgtagtttt ataactttat
     2101 taatctttat tttgatattt gtttcagtta taggctcagt tataaaatta tggccatatg
     2161 atttaagctt tacaatggaa cactatacgt ttaaaggtat atcaggaaat ggaatcaaaa
     2221 catatttaag cagtttaaaa atatcaattt taacagcaat atttggaact atatttgtat
     2281 tttttagtgc ttatatgata gaaaaagtgg ataagtttcc tagactgaga caaatatctt
     2341 attttttatc aatgatacca atggcattac ctggtttggc tttaggtata tcgtatataa
     2401 cttttttcaa ttcagcaagt aatccattaa attttttata tggaagtaca acaataatgg
     2461 ttatagccaa tatagttcat ttttattcag tttgctttat aacttcaaat tcatctttaa
     2521 aaatgttgga tagagagtat gaattagttg caaaatctat aaatattcca ttttataagg
     2581 tgttttttta tgtaacactt ccaatgtcca taacatcaat acttgaagtg gctgtatatt
     2641 acttcgtaaa ttcaatggtt actgtttcag cattaatatt tttatatata cctcaaactc
     2701 aaactgcatc aatatcgata ctaaagttag aagagatagg gtatataggg ccatcagctg
     2761 ctatggcagt attagtgttt ttaacaaata taatagtaaa acttttgtat gaattgacaa
     2821 caaagaaatt aaaaactaag acacaaaaat ggcaacaaaa agaagttgca tagaggaggt
     2881 aaaaatgaaa tttaggaaat tagtctcttt aggaatatta tcaacacttt tattgagtgt
     2941 aataactgga tgtaataatg cttcatcagg ttcaaattta aagtctaata ataagataaa
     3001 aggagagtta acagtttata cagcaattga agaagatagt atagatacat atttaactac
     3061 atttaaagag aaatatccag atattaaatt gaatgtactt agattatcag taggagatat
     3121 aacagcaaga ttattagcag aaaaagataa tcctaaagca gatgtgttat ggggggttgc
     3181 agctacaagt ttgataattt tagatgacaa agaaatgcta gagccatacg ctccgaaagg
     3241 ctttgataag gtaaatcctg tatttaaaga tgataagaaa gttccttctt gggttggaat
     3301 agatgcttgg atgactggta taacagtaaa tactaaagaa ttaagtgata agaacatccc
     3361 agttcctagt tcttatgaag atttaataaa gccagaatat aaaggtctta tatctatgcc
     3421 taatccagct tcttctggaa caggatattt aacagtatca gaacttatac aaattatggg
     3481 tgaagaaaaa gcatggcagt atatggataa attgcataaa aatattggtg tatacactca
     3541 gtctggttca gctccagcta tattagcagc ttcaggagaa tatcccattg gtatatcttt
     3601 tggaaataga ggtataaagt taaaaggtga aggttatcca gtagaagtag tatttccaaa
     3661 agaaggttct ggatgggaga tagaatcaaa tgcacttgtg aaaaaagata atataaaaga
     3721 agcatcaaaa gtatttttag attgggctat aagtaaagat gcaatgaatg agtattctaa
     3781 gaattttgca gttactacaa ttgacacggg aaatccggtg ccagaaggat ttccaaagaa
     3841 tccattagaa cagatgatag ataatgattt aaaatcagca gcaagaaata gagaggctat
     3901 actaaataaa tggatagaaa aatatgatgg aaagacagaa aaagagagct aagccttgca
     3961 ataaaattta gtacaaaaag cgcctagaat ataagtttta tatctaggct cttttgatat
     4021 atttatttaa aatgcatatt tctatataaa aataaatagt ttagtacctt gattaaatta
     4081 aataaaaacc ttaaaataaa gagatgtaaa gaaaaactaa aaacgaataa aaggtgaatt
     4141 tatatgataa aagaaataat agtagtagag ggtagagatg acgttacagc agttaagaga
     4201 gcaatagatg cagaacttat aactacaggt ggatttggat ttccaaaagg tgtaatggag
     4261 cgtataaaag ctgctaatga aagacgtggt gttataatat ttacagaccc agattttgca
     4321 ggtgaaaaaa taagaaaaaa aatagcatca gaagttccag gatgtaaaca tgcatttcta
     4381 ccgagagagg aagctaaaaa ggatggagat ataggtattg agaatgctac tcctcaaagt
     4441 ataatagcag ctctgaataa agttagaact gaaaatacag aaaaaagaga tgaatttaag
     4501 caagttgact taattagaaa tggtcttata ggtaatgaag aagcatcact tagaagagat
     4561 gcacttggaa aaatacttgg aattggatat ggaaatgcaa aacaattttt aaatagattg
     4621 aataattatg gagttgaaag agaagaattt atagatgcat taaaacaaat ctaaatgtaa
     4681 tgtttaatgt atgaaaaata tgtatataaa ataaattgtg gaattacggt taatttaaga
     4741 taaattattt ataatatata ccttatttta aatcaatgta aatacatata aataagtata
     4801 attaataaaa ttaaatatca aaataagtta aaaaggagta aataatggat agactatctt
     4861 cacataatgc aacaaaggaa gtagtacaaa aatataattt taaattttca aagtctttag
     4921 gtcaaaattt cttgatagat agtaatataa ttgataaaat cttatctgga gctagaatca
     4981 caagaggaga taatatcata gaaataggac ctggtatagg aactcttact cgtgagatgg
     5041 gtaaaatagc agaaaaagta gtggctatag aaatcgatag aaatttaatt cctatattga
     5101 aagatacctt gtcagattta gataatacag aggttgtaaa tcaagatata ctaaaagtag
     5161 acatacagga attagtaaaa gataaattaa atggtgggcc agttaagctt gtagcaaatc
     5221 ttccatatta tataactact ccaatagtta tgaaattttt agaggaagac atacctgtaa
     5281 cagacatagt agtcatggtt caaaaagaag ttgcagatag aatgaatgct attccgggta
     5341 ctaaagatta tggggcatta tctatagctg ttcaatacta ttgtgataca gagattgtgg
     5401 caaaagctcc aagacatatg tttataccac aaccaaatgt agattccaca gttataggtt
     5461 tacatgtaag agataaaaga aaatatgatg ttcataatga agacatattt tttaaaacag
     5521 taaaagcatc ctttggacaa agaagaaaaa ctcttcttaa ttcgttggga ggtcttggtt
     5581 ttcttaataa agatgaaata agagaaatat taaaagaagc taatatagac gaaaaaagaa
     5641 ggggtgaaac tcttagtata gaagagtttt cagttctttc aaatataata aatacaaaag
     5701 tatcttctaa atagaaggta ctttttttaa ttttgttaac atatattgtt tagagaaact
     5761 gtgttaaggg gggattgcag tgggttctaa aagaaagttt aaaagagact atataatact
     5821 agaagctaaa gatataaact ttagatacaa agaacgagta ctgccaaaag cttttgcaaa
     5881 agttgaagta aatgatgaaa aagcaattat tgcattatat gtagaaaatc taaaatatgt
     5941 taaagatggg tataaagcag tagcaataaa agatgaccat ggattaatag acttaggaaa
     6001 tataatttta aatgaacaag gaagaggtga atttgtttta gatttagaag attatgatga
     6061 tgagataaaa ggtattgcat tattgtatga aagaaatata cctttaattg gatttaaggg
     6121 aaataagctt gaaaattatg aggacatatt atttcaagag gtagaggaag aagaatatga
     6181 agaatatgaa gagttggaag aagaagacga agagatagag gataatgagg aaagtgatag
     6241 agacgaagat agtgaagaag aatatattat agaagaatat gatgaagatg agtatgaaga
     6301 ctattgggaa gatgatgaag atagtgaaga aggagagtta aaagcaagaa cggaagtaga
     6361 agattctggt gatgaggaaa tagaagagtt caataattat aaggaaatag aaaattataa
     6421 tagggttaaa gtacaggaag aatatataga catagaatat gatgaggaag aagaggaaga
     6481 agaagttttt gaagtaaata gagaagaaga taggtttgtt tcctcaaaac ctactaaatc
     6541 aaagaaagtt caaaaagatc agaataatat tgaaaaaata aaaagagcta atggagtaag
     6601 tcaaggagag agtggaaata aacaaaccag tacattactt atgccaagac aaataaaaaa
     6661 aggtttgaaa ttatttaaag aagtaaaacc ttttgtaaca gattatatag ataagacaag
     6721 atggtggaaa atagagataa atcctacaac actgtgtgga tatacaatgc cttatttagg
     6781 atatttaaac gtattgaatt acactatgta tagtgatgca gttttacatt catataaata
     6841 cagacactat ttatttggag ttcagtatga tgaatataat aagagaaaac attatattta
     6901 tgctatccca ggaaatagaa atgaacagcc agacaatgga agcacaggat ttttaagata
     6961 tcaagcttgt gataatagaa caaatagttt aggatattgg ctatgtttta tagattctag
     7021 acaaagaaat atagttaggt aaaaataata aaaatgaaaa aatttagact caattcttat
     7081 tttactgaaa aaattaatga aatgtagtac aaaactttaa aaactattga taatatttaa
     7141 atattacact acaatatatc ttgaaatacc tattatatta tggaggaaaa ttatgataaa
     7201 cgagcaaaga attttaaatg agtttcttga attagtacag atagatagtt tatctttaaa
     7261 agaggggaat gttgctaaag tattagttaa aaaattagaa gaaataggtt gttcagtagt
     7321 aatagacaat gctggtgaaa aagcaaatgg agaaactggt aatataatag ctacattaaa
     7381 aggaaataaa gaaggaaaga aaatattatt tagttcacat atggatacag taactccagg
     7441 tataggggta aaacctatag tggatgaagc aaatggaata ataaaaagtg atggtactac
     7501 aatattaggt tctgatgata aagcaggtat agcagctata ttagaaggct taagatatat
     7561 aaatgaaaat aacatagaac atactgatat acaagttgta ttctctatat gtgaggaatg
     7621 tggattagtt ggagctaaaa atttagacta tgaaaaaata gattctgaat atgcatttat
     7681 attagatagt ggtggttctc caggtgaaat aatagtaaaa gctcctgcac aagatgtaat
     7741 aaatgttaag atattaggaa agactgctca tgcaggtctt gaacctgaag ctggaataag
     7801 tgctataatg gtagctgcca gagcaataga aaatatgaat ttacttagaa tagacgaaga
     7861 aacaactgct aacataggaa ttataaacgg tggaacagct acaaatatag taacaggaga
     7921 agttaatata attgcagaag ccagaagtct aaaagaaaat aaattagatg ttcaaactaa
     7981 acacatggta gagacttttg aaaaagctgc aaaagatttt ggagctcaaa ttgagataga
     8041 tgtcaataga gcatatacac caatagatgt tagtgaggac tctgaaataa taaaacttgc
     8101 aaagaaggct ttttcaaact taggtataga aggtcatact gaatctacag gaggaggtag
     8161 tgatacaaat atattaagta agaatggtat cgaagctata actttaggaa ttggtatgaa
     8221 aaatgctcat acactaagtg aacatatagc tataaaagac ttatatgatt ctgctaaaat
     8281 ggtagtggag ataataaagg aagcataaat ttaaataata gtgtataata tatatatcgc
     8341 ctagcttaag ctaggtgatt tttcatttta tagaagattg ataatctcta ttgaaagcaa
     8401 agagtataca tttgcatata agagagtgtt taatttatgt tcaaatgtag gttcttaata
     8461 atatttagat tgtttcataa gggaggaata aatatggaaa caaggtatga agaagcaaat
     8521 aaaataacaa tacaatcaat actttggaat gtagtattaa caataattaa ggttatagct
     8581 ggtgttattg gtaattctag cgctatgatt gcggatggat tacattcagc atcagatatt
     8641 ataagttcta taggagtact aataggcaat tatgtatctt ctagacctgg agatagagag
     8701 cataattatg gtcatgagaa agcagagaca ttagtatcat ttgtgttatc aatattgctt
     8761 attttcgttt ctataacaat aggtatagag gcaattaaat ctttatttaa cttagatgca
     8821 ttaagtgttc catcaatatt accattagta gtatcagtta tttcaatcct gataaaagag
     8881 tatcaatata gaataacaat aaaagttgct aaaaagataa attcaccagc cctaaaagct
     8941 gatgcttggc atcataggtc agatgcttta tcttcagtag ctgcttttat aggtataggt
     9001 ggttctattt tagggtttaa accattagac ccaattgcgt cagttgtagt ggctatattt
     9061 gtagctaaag taggaattag tatacttata agttctgtta atgaattgat ggatgtttca
     9121 gttgatgaag aagagataaa agaattaaaa tttattgtag cagatacaga aggtgttaaa
     9181 aatcttggtg atataaagac tagaaaacat ggagctatgg catatgtaga tttgactata
     9241 tgtgtggatg aaaatttgac agttaagcaa ggtcatgata ttgctaccaa gcttgaaaaa
     9301 catataataa aacatatgga atttgtaaaa ggcataacag ttcacgttga accatgtact
     9361 aattgccaag gaaataagtg taataattaa ttaaaactta aaaaaaagtg taaaaagtta
     9421 tataattgtt ttatgattat aaactctagg ggtggttaaa tgagcaagaa aagaaaacta
     9481 aagaaaaaag ctgcaatatt aataggagta gttgttttct ttataatata ctttatatcc
     9541 tttagaatag gaataaatat ggaaaaggat aaaagtgaag tgccaaagaa ggtagaaaaa
     9601 gaagaagaag ataataggtc tttaaggact caaataaaag agaagaagaa ggtttatata
     9661 tcagataaaa atatagaaaa cataaaaatt gaagaagata tgtgggatga aataaagtta
     9721 tttttctcag attttaagaa agtaagaaat actacttctt cttacgaagc agtttatgaa
     9781 ggttattctg atgatggagt taaattttct actgacctta acttttttag agtatatact
     9841 gtaaataaag aagaatatta taaagtacca gttagttcta aggatgcttt taagaaactt
     9901 ttagatgaga gtatatatac ttcgtttgac cttttaagtc attataaaga ttggaaagag
     9961 gttactataa ctcatggaaa agaaattaaa aaagttcata gatggaaatt tgatgattta
    10021 gcacataaga tgatttcaaa gagggttgta ggaaaagttc aacctgagaa aagtaaggaa
    10081 agaagtgact ataattttac tataaatata aaaggtgata attttgatgt aaaagttgat
    10141 acaatgggaa aagattatgt aaaagttact tctaagaaaa atgtatctta ctatgaggtt
    10201 catacaagtt tatttgatta ccttaaaaat gagatatttg aaattggaaa gaaataagac
    10261 tttaagaagg ctatcaaatt agatatttga tagcctaaat tattttatat attatttttc
    10321 aaccaacaag tctgcaacct tataaattgg accagcacca gcagttatta ctaaatcatt
    10381 atccttaaca ttttcacgta agtattttac tatatcttca aattctttta tatagatggc
    10441 atctacattg ttttgatata atttctcaac caagtcctta gaatgtatat ctcctgggtc
    10501 tttttcccta gcagcatata tatctgttat gataacttta tcagcagcat agaatgcctc
    10561 tgaaaattca cctaaaagag attttgttct agtataagta tgtggctgga agatacacca
    10621 taaagttgat ttttttaatt ttttggcagc agctaaagtt gcttttaact cagttggatg
    10681 atgtgcataa tcatctacta ctaaagcatt cttatagtac cctttagttt caaatcttct
    10741 tccgacacct ttataaattt ttatattttt tcttatagtc tctaagtcaa taccagaaac
    10801 aagagctgcc attatggcag aagaagcatt gtatatgtta tgaagaccat atacagaaag
    10861 ttcaaattct cctaaatctc ttccttcata ttctattcta aatattccat gaccactatt
    10921 gtcaaagtga atatctttta ttacagcatc attgtcagaa tctctgccgt attttataat
    10981 agtagcttta acatcataca atatgtcatc aatattttca tcatcgccat ttattatgaa
    11041 atagccttct gggggtaata attttccaaa tttgttaaaa gaagccttga tttcatctat
    11101 tccagagaaa taatcaagat ggtcttcttc aacatttaaa actatagata tctttgggtt
    11161 aaaattaagg aaactatcta catattcaca tgcttcagtt ataaagtggt tggaattacc
    11221 tatcttaaca ttaccaccta tcatacttaa gttaccacca actagtatag ttgggtctaa
    11281 gtctgcatat tcaaagattg tagataacat agatgttgta gaagtttttc catgtgttcc
    11341 agaaactgct atagaatttt tatattccct catgatttgg cctaaaaaag ttgctctgtt
    11401 cattaccaat ttattttttt ctttagcagc cacaagttct tcattgtctg gatgaactgc
    11461 tgctgtataa acaaccatat ttacatcatc tgatatatta tcttttttct gaccaatata
    11521 aatagttgcc ccttggtccc taagtttgtc taataggtat gattcgtttg aatccgaacc
    11581 tgaaacttga taacctttat tgatacatat ttcagctaat gcactcatgc tgataccgcc
    11641 aattcctata aagtgtatat tcataataaa accacctttc tttttaatta agtcctacaa
    11701 tatgaactat ttctgtggta taataatata atgttcaata ttttataatt aatatgcata
    11761 taatattata tgtattttat ttttgaatat aataaaacat aagtaatatt ataccacaat
    11821 aagaattgtt atgcattagt tagtttaacg ttatataaag gtacttgtag gaggaaataa
    11881 taatgaaatt taaaagaaca gagagaattg gagcaatagt taaaatactg tctgataatc
    11941 caaataaaat atatacttta agttatttta caaatcaatt taatgcagca aaatcaacaa
    12001 taagtgaaga tttattagtt gtaaaaaatg tatttgagaa gctacattta ggtaaagtta
    12061 taactatttc aggagcagca ggaggagtaa aatatatacc taaaacttct atagcagaaa
    12121 atgcagagtt cttaatggaa ttatgcgaga gaatatgtga caagtctaga atactatctg
    12181 gtggtttttt atacttgatt gacctaattt acgaccctac aatagctgct aagataggaa
    12241 agatattcgc atcaaatatt gagtatgtag atgcagatta tgttgtgaca atggaaacaa
    12301 aaggtatacc tatggcactt atgactgcaa aagctatgaa tttacctcta gttataataa
    12361 gaaaagatat aaaggtatca gaaggtccta cacttagcat gacatatgtg agtggaaaca
    12421 gttctaaagt tgaaagtatg agtttgccaa gaaaagctct taaaccagat agtaaagtta
    12481 taattataga tgatttcatg aggggtggag gtactataaa aggaatggta gaccttatga
    12541 atgaatttgg tgcagaggtt ataggtactg gagtattcat atctacaacg aatccttcag
    12601 aaaaaatggt aaaagattat atttcattga ttcaattgga tgttaatgga gataaaatag
    12661 tagtagaacc aaacttaaag acttttaaag atgaatatag aaatgaagat ttagacgaag
    12721 aagaagattc agaatttgaa tttgagatag atgaagatta ataaaaaaaa tatttagaaa
    12781 aatatgtaaa aataaaaaga ggatatccct agttgttcat agaattattt aaaaaacagt
    12841 ttatattaat ttattccagc gataataatt ttaatataaa ttaatttctg tcaaaatcaa
    12901 agggggatat tttggtatga agataactga cgtaagagta agaaaattaa cagaagaagg
    12961 taagatgaaa tgtatagttt caataacttt cgataatttg tttgtagtgc atgatataaa
    13021 agttatagaa ggccataatg ggctatttat agcaatgcct agcagaaaag taggcgaagg
    13081 aaatttcaga gatatagctc atccaataaa tgcagaaatg agacaagttt tagaagatgc
    13141 agttttacaa gcgtatcatg aagcattagt tcaatgggaa gtagctgctg aataataata
    13201 aatatataaa atggagagag cccgaacatt taggctcttt ttaaatgtag aatttctatt
    13261 aaaatatgta ctttttagta aaaaagtgct atattattag tgataatgta tatgttgtat
    13321 acaaattgtt agataaatgt attataatgt atattgatta agagaataat acgaataaaa
    13381 ttaaattaga ttgaatggag tgtatcaatg aactttaaag ctataatcct tgctgctggc
    13441 aaaggaacaa gaatgaagtc taaataccca aaagttatac ataaagtatg tggaaaagaa
    13501 atggttaacc atattataga tgtttcaaaa aaatcagggg taaaagatac tgtcgtgatt
    13561 ttaggacatg aagctgatgt agttaaagaa aagcttgcag aagaaattat aatagccatg
    13621 caaacagaac aacttgggac aggacatgct gtaaagatgg ctaaagaata tattaatgat
    13681 gaagatacaa tagttgtact ttgtggagat actccactta taaaagaaga gacactaaag
    13741 agactatttg aatatcatat agaaaataaa tatcatgcta cagttcttac aacaagagtt
    13801 ggaaatccaa caggttatgg tagaataata agagataaga agggtgacct tcttaaaata
    13861 gtggaacaaa aagatgcaaa ttcagaagaa aagatgataa gtgagataaa ctctggaatt
    13921 tattgtttta atggaaagag tcttagagaa gcactagatt tgctaaataa taacaattca
    13981 caaggtgaat attatttgac tgatactgct aaaataatga gagataaagg attaaaagtt
    14041 ggtgcatttg caggttcaac tattgaagaa cttatgggcg ttaactcaag agtagaatta
    14101 tcaaaggcag aagaaattat gagacgaaga ataaatgaat ctcatatggt aaatggagta
    14161 actataatag acactaactc tacctatata gaatctgatg ttatgatagg taatgatact
    14221 ataatatatc caggtgttat gttacaagga aaaacaagga taggttcaga ttgtataatt
    14281 ggaatgaatt catctataac aaattcagaa ataggtgatg gtacagaaat aaaaaattca
    14341 actataattg atagtaaggt tggagaaaat tctactgttg ggccatatgc atacttaaga
    14401 ccaaagagtg acttaggaaa taatgtaaaa ataggcgatt ttgtagaagt aaaaaatgct
    14461 attatagaag atggctcaaa agcatctcac ctttcttata taggagatgc acatgtcggt
    14521 aaaaatgtaa atattggatg tggagtagta tttgtaaact atgatggtaa aaataaattt
    14581 aaatcaatag taaaagacaa tgcgtttata ggttcaaatt ctaatttagt tgcaccagta
    14641 gttgtagaag aaaaaggata tatagcaaca ggttcaacaa taacatatga tgtcccagat
    14701 ggtgctttgg caatagcaag agaaagacaa gttatcaaag aaggatgggt agaaaaaaag
    14761 aatcaaaaag acgaccaaag taagtaatta tgtagtttat tattttggct aaaaaattta
    14821 gctcgaaatt attataaatt atattcatta gtattttaaa attttcagga gggttacata
    14881 aatgaatact agcggaagcg agattaaaat tattgcaggt aattcatcga aagagttagc
    14941 acaaaaaata gctgactata taggtgtatc agtactagat tgtgaagtag gtacatttag
    15001 tgatggtgaa atatgtgtaa acatgaatga gacagtaaga ggttgtgatg tgtttgtagt
    15061 gcaatctact aacagcccag taaatgataa cttaatggag ttattaatct taattgatgc
    15121 gttaaagaga gcatcagcag gtagaataac agcagttatt ccttactatg gatatgcaag
    15181 acaagacaga aaggctaagg caagagatcc aatcactgct aaattagttg caaatttaat
    15241 aactgctgct ggagcagata gagtattaac aatggattta catgcagctc aaatacaagg
    15301 atattttgat ataccattag accacttact aggtggaaca atattagcaa attattttaa
    15361 tgaaaagaaa atagaagatt tagtagtagt atctcctgac ttaggaagtg ttactagatc
    15421 aagaaaattt gctaatactt taaatggaga agttccaata gctataatag ataaaagaag
    15481 accgaaagct aatgtatgcg aagttatgaa cttaataggt gatgttaaag gtaaaaatgt
    15541 tatactatta gacgatatga tagatacagc aggaacaata gttaatgctg caaatgcact
    15601 taaagagttt ggagcaaaag atgtttatgc ttgttgtact catggtgtgt tatcaggtcc
    15661 agctattgaa agaattgcta attcagaaat aagtgagtta atagttcttg atactataca
    15721 acttcctgaa gagaaaagaa tagataagat aaagataaaa acagtagctc ctttatttgg
    15781 agatgctata agaatgatat tctctaatga atcagtaagt aaattattct aatttattgt
    15841 atttatgaga gttgtttaaa aggatataaa ccttttgagc aactcttttt tgattttata
    15901 attatggaat gttatttttt tcttgttaga aaggtatata ttattgtaaa aagagattat
    15961 agtttaaaat tatgtatatt taagtaaaat aaacttaata aagggcatat tattcaaaat
    16021 attatgaata taaaaaatat tacaaaaaag tattgaaaaa attcagatta tgtagtatta
    16081 tgtaaatata aataaagaaa gaggtgtttc caatgggaag gttagaagat gaacccctaa
    16141 taattaaatg attcagtcac aatctaaagt gaatataaaa atctattgaa agtttattta
    16201 taagaattga aaatagtatc tattctatct ttagtgtttg tgagagggtt ctggattata
    16261 taagattact gtaaagaagt aattttgcac aataagcata tctgtaaatg aagagtttcg
    16321 tttatataat gattgtggct gaatcataac tataatagat agaatgttta taatagatga
    16381 ttttataaaa tactatattg taatagcttt tagctttcaa ataaatatta gcaaaggatg
    16441 gaaatgacaa tgataaaact aaatgataat ttataaatat ctccttacga tatataataa
    16501 ttgtagggag atgtttcttt ttttgtatag cataattttt ctagaaattc tgtttcaata
    16561 tgtaaaaagt tggtatatat aaatagtata aacagtgttg ctaaaatatg gtagatatta
    16621 tataatataa gtggatatgt atatacatta tgagttatat aatttgaatt taataaaata
    16681 gaaacgttga tttatgttct gtaatgtggg caccttggag catattgagt tagtggtgca
    16741 accggctatg aaattgtatt tatttataaa tactattatt ttcatagctt tttttattta
    16801 aaaaatggtt atatgttaga aataaatttg gttagtacgt ttaaataatt ttacttagat
    16861 ttttaaccag atatattgta agtaagtatt aattaaaaaa tttaaggggg aataaaaatg
    16921 aagttaaaaa agaataaaaa aggtttcact ttagtggaat tattggtagt aattgcaatt
    16981 ataggtatat tagcagtagt ggcagttcca gctttattta gtaatataaa caaggctaag
    17041 gtagcaagtg ttgagtctga ttatagctca gttaagagtg cagcattatc ttattattca
    17101 gatactaata agataccagt tacaccagat ggtcaaactg gtttaagtgt tttagaaact
    17161 tatatggagt ctcttcctga taaagctgat ataggtggag aatataaatt gattaaagtt
    17221 ggtagtaaat tggtattaca gataggtaca aatactgagg gagttacctt aacagaagca
    17281 caatcagcaa aattattgag tgatataggt gaaaataaaa tatatacaaa tgcagctctt
    17341 agtgctaaat taacatctac tacaaaggta aataatgaag ctttatatat agttcttata
    17401 gataatattg taatggatca acaaggagct taaaagtaaa ttgttataat gaaaaatata
    17461 ataaaaaaac aggaagaaaa tcttcctgtt tttttatact attttcttta atgttcaatt
    17521 atgaatgatg atataattga gatagtaaag gagtgaaacc tgtggctaaa aaagttagaa
    17581 taggagacaa actggtcgaa aaaggatata taacagaaga acagcttaaa tgggcactat
    17641 cagaacaaaa aaacagtggg aaaagactag gagaatttct agtacaagaa gggcttatag
    17701 atagtaacct actaataagt gtattaaaag aactcttgga tatagaaagt atatttttag
    17761 aagggacaga aatagacact ttagctacaa agatggttcc agaaaatata tgcaaaagat
    17821 atactgtatt tccattcaaa atagatggaa ataaaatatg tctagccatg tcagacccac
    17881 aagacagaga agcagttcaa gatgtaaggc gtatgtcagg taaagacgta gagatattta
    17941 tatcaagtac agaagatata aacaaagcaa taggtcatgc ttatgcacat tcagaaataa
    18001 ataaagcaat gactgagtac aacaaaaata gaactggtgg agtaagagaa acagtaatac
    18061 ttgaagaaga tgtaaatgct gctccaatag tacgattagt aaataacata ctagaaaatg
    18121 ctgttagaat ggaagcaagt gatatacata tagaacaaag tgaaaattac atgcgtgtta
    18181 ggtttaggat agatggaatg ttaagagaat atatgaggat gaactcagca ccatataagg
    18241 cagtaatcag ccgtatcaag ataatgtcag atataaatat atctgaaaag agaataccac
    18301 aggatggtag aatctattta aaagtggata ataaacccat agattttagg gtttctacaa
    18361 tgccaactaa cagggatgaa aagatagcta tgagggttct tgataagagt aattttatgg
    18421 ttagtaaaga agtactagga atagacgaac atggctctaa gatatatgat gaattgataa
    18481 acacacctta tggtcttata ttggttgttg gccctactgg aagtggaaaa accactacat
    18541 tgtattcaat gttaaatcaa ctaaatacag aaaatagaaa tctattaaca atagaagacc
    18601 ctatagagta tgaattacct ggtgtaaatc aatctcaaat aaatgaaaag gcagggctta
    18661 ctttcgccag cggacttaga gcttttatgc gtcaagaccc agatataata atggttggag
    18721 agataagaga tactgagaca gcagaaatag caattagagc ttctcttaca ggacatttag
    18781 ttttaagtac attacatgca aatacagcag ttggagcaat ttcacgtctt ttagatatgg
    18841 atgtggaaag ttttttaata acttcatctg ttttaggtgt aatatctcaa agacttacac
    18901 gaaaaatatg tgagcattgt aaggtttcat atgaagcaga tattggagaa aaaaaggctc
    18961 ttggtattga tgtaaatgaa tcagtaacta tttatagagg aaaaggttgt gaaaggtgta
    19021 ataatactgg atataaagga cgtctaggta tatttgaaat gcttgaaatt actccagaaa
    19081 taaaagagtt aatagattca tctgcaaatc aaagagaaat tttaaagatg gctagaaaac
    19141 aaggtatggt tagcttaaaa gaggatatag ttaaaaaggt tttaaatgga aagacaacag
    19201 tagaagaaat gattagaata attttgatga ctgattaaag gatgatatta tgaaaagttt
    19261 taaatacaca gtaataaaag aggacggaaa aaagcaaact gatgtgattg aagccaacga
    19321 ttttaatgaa gctagaaatc ttcttagaaa gagaaatctt agagttattg aaatcaaaga
    19381 aagcaaaaat aaaaatataa gattatctag tagaaagaga aaaaaagact taggagctga
    19441 ccagataagc cacttttgta gacaatttgc tattatagta tcttctggta taaatagtat
    19501 ttctggatta gaaacattag ctagaaggtc aactaacatt acattaagag aagaaattaa
    19561 tcgtatagta gcagaaataa aagtaggttc tactattgca gattcaatgc tttcatcaaa
    19621 atcaaagttt ccaaagttac ttggtgctat ggtagctaca ggtgaagcta caggtaaatt
    19681 agatgaagta ttaaaaagta tggcttcatt ttatgcaagt gaacacaggg ttaaacaaaa
    19741 gcttagaaat gctgcaacat atccattaat agttttgatt tcttcattta taatgatttt
    19801 tatatttaca acatttatgg tgcctaagat gattaattca attacaacag taggagcaac
    19861 tcttccactt ataacaagaa ttgttatggg gtttggtatg tttatgaaga agttttggat
    19921 aatagtactt ttagttatta tattatttat ctatcagttt aaaaaatatt taaaaacgtc
    19981 agttggaagg gctcataaag atagagttat aaacaagata cctgttttag gaaaaggaat
    20041 aaattgtatg gtagctacac gattttcaag agcattgtat ctatttgtat caacaggata
    20101 cccattagtt caagggcttg attatattat agatagtgta aataacacta tggcagagaa
    20161 gattttagca tcagctaaag atggaataac aagaggagaa ggcttagcag aaaatttaga
    20221 gagatatacg tattttgact ctgtgttggt acagatgata gctatagggg agcagacagg
    20281 ggaattagag aatataagta gacagatggc agaattttat gagtatgaat ccgagattta
    20341 tttaaatagg atggcctcaa tgatagagcc tgttttaata attgcagttg gtattatagt
    20401 tgcgatattg gttgtatcaa tatttatgcc aatgcttagt atatatgatg ccatgtagga
    20461 gggaaaggac aaatgaacaa ggtttatgta tcatattatg gcgattctat ttccatagtt
    20521 gagggcagat ataaacacga taaggataag ttttatgtaa aaaattttaa tgtgttttca
    20581 atagaggata ttgcacaaga tttttctaag gaaaagtatg ctctattgag atacgcatta
    20641 gaaaatgtta agttaaaatc aagacatgta gttttttgtt taaatacaag agatgtaatt
    20701 ttaaaacccc aaaaacttcc taatatggac aaaaaggatt tagacggatt tatgaagcta
    20761 gagatggacg aaatgatggc tttggagtca gatgaatatg tattttcgta tgaagtatct
    20821 agtgaaacac ctgataattc agatggcgat gaatcaagct taaatcttat tattggagct
    20881 atacaaaagg aagaggtaaa tgcaattgta gagataatgc atgaatttga tttagtatta
    20941 gatagaatag atacattatc aacagcttac ttaagaatgc ttaaaaattt agactacaat
    21001 gatataatgg taaccaatat aagtgacaat agcacaatta taaatatata taagaaagat
    21061 actttattta ttagtgataa tatacctata aagataactg ctaaagatat ggacatacaa
    21121 ttattaaatg tagtagaaga atcaaaaggt cttatgaatt actattcgtc aagaaacttt
    21181 ggtaaggtta cggatactat attaatatta ggaaaaagat attccaataa attaatttat
    21241 gaaactttta gctctgcatt ttcaagctat gtttctcaag gccttactga gataatggat
    21301 atatctgata tgataactgg agatatagat gaagatgata taaattcaat tgtagagctt
    21361 ttaggaagta tgatagaacc taaagggaaa agagattttg aaaatataaa ctttttacca
    21421 atggatatac ttagaaaaca gataaaagct aaacaaatga aaaacatact gaaaattgct
    21481 ccaatagtta ttttgattct tgctatacct tatttatgtt taattttttc aaataattta
    21541 gcaaataagg aacttgaaaa tgttaatggt gaaataagaa atattgaatc tgcatattat
    21601 cagattggaa atatagaaaa aatgatacag agtaaaagcg aagaaataaa ggtatatgat
    21661 atgttaattg ggaaaaaggt aaagtgggaa aatttattgg atgatataga taaaaatact
    21721 ccaagtaaag ttgaactaac aagtttatca acaacttatc aaaatcttgg aactaataat
    21781 aaagctgata attcaaaaag taataagact acaacagata aatcaagtaa caatgaaaca
    21841 aaatcaagta cgaaaagtga tagtaatgga gataaaacag ataatgattt aaatgaagat
    21901 aagactacaa atgtgtcaga agcattaact ggaaatagtg agactaatag taataacaca
    21961 gataaagatg ataaaactcc gttgtatgat aagataccaa atgttataaa tatagaagga
    22021 aatgctaaag attcctctta tgtaggtcaa tttttatata aattaaaggg attatactat
    22081 ttcaaggatg ttaaattaca tagcataaaa agtaatgcag aagattctaa ctatacattt
    22141 agtatgacat tatacctgaa agaaggtgta ttaactaatg aataaagata tatttaaaaa
    22201 agatataaaa gatatattta aaatggaagt tagtctaaaa gggattctta taaaggttat
    22261 aattttatta gctattttat catatggtta ttttggactt taccctcaat atcagaaata
    22321 tattgctaca aaaaattctg taaaacaggc tactgaaaaa ttaaatatat ataaagagaa
    22381 aactgcactt atgccaaaat atgaaaaaca acttgaagaa gttgaaagtg aattaaaaga
    22441 aaaaagcaaa aatcttttat atgatatgca agatggtctt tttttaatag gtttagataa
    22501 aaaaatgaag gcttataata ttgacttaac cagttatgac gttggaaatg ttaaagaata
    22561 cgaacatttt tatggtatat caacaacaat aagtgttaga ggtgattata gaaatgtcaa
    22621 aaaattaatg gcttatttag aagaacaaaa aaatgttact caaatactag attatacaat
    22681 gacttcatat atagaaccag aaaaagaaga ggaagaaaca acaaataata aggatttaat
    22741 taaaaatgtg aacataaata aagatacaaa aatatactgg gtggaaggcg aaaataagtg
    22801 gcatttatat aaggattgtc cattgataaa agagggagag attctaaaaa atgacaaact
    22861 tttagatagg tctactaatt atctatgtaa tacttgtgta cttagagctg caacagaaaa
    22921 agaagcaaaa aaagaagtcg tagctccaaa ggcagaaggt gtagtagatg ctacatttaa
    22981 atttataatg tacactaaag agaatactac aatgatactt gatacagata atccatcttc
    23041 atggagacca ggaaaatata atccatttaa gtctagtgcg aaataaagct aagtaagctt
    23101 taaagatagg tgatgagttt gtacaaaaat aatgaaaaag gtcttacttt attagaagta
    23161 ataatagctg tttttatact gacaatagtt ttaagtattt cttataaagt gttcaatggt
    23221 ataacatcag cagtaaaaaa gcaacagatt attacagatg ctcaagtaaa tattaatcta
    23281 attaataagt atctaaatag agatttggaa aactgtaaag aactaactaa aactggttca
    23341 ggtaataatt atgaatacaa tatagagatg ccagataatg ttgtaaaata tgaagtttct
    23401 atagaaacta aaaaaaatac agaggtatat tctgtaacga gaatccaaaa caatacaatt
    23461 gatacagaaa atgaagttag agaagagata atctacaatc aaccactagt tcaaaacaat
    23521 aaagaaatga aggaaacacc atttaaaata gaaaagcaaa ctggtaaatc tatttatact
    23581 gtaagtatat actataatga atcagtgcaa gaaagtcata aaaatagcaa tttaaataac
    23641 aaaacttata catttgatgt tatgtcaaga atagggtaat aagaatttat taaaaaataa
    23701 gtaggttgat aaaatatgag taaaaagaat agtgaaagag gattttcctt aattgaagtg
    23761 ttagtagcta tggctattat gggaattgta ctatttgcat tttttaatat tatcaatact
    23821 aataacaaag caaacactaa aaatgacaca gatataactt ccttaaacta tgtccaaagt
    23881 gaaatagaaa atctaagaga aaagataaaa agtggagaat ttgattttga tagtttagat
    23941 aaactagaag atggaactgt cgtatatgaa aaattaatag ataaatcaaa aaaagttgta
    24001 tatgataaag ttcttagtga aggtgatgtg agcttatatg atactccata tgaaaagatt
    24061 acaacaataa aagatgaaga tggtaattta atcgacaaag gaaatattac aaataagata
    24121 aagactattg tagaagataa aagtggtcag atatataaaa tagctgtaac tgggaaaagc
    24181 atgaatgact attcaagtaa aaaagaggtt aaaattgtaa ctgaaatatt taaagataaa
    24241 taattttact tgggggtgag attttgagga agtggaataa atttaaaagt gaaagagggg
    24301 cagctttagt tttagttttg gttgtagtag cattgctttc tattgttgga ttgatatttt
    24361 caaatcagat agctaataga ataaaatcta caaagactac taatgaaggt atacaagcaa
    24421 agtatttagc agagacatgt gtggaaaata gtattgataa agcatatgaa aaattgtatg
    24481 atgagcttga gaaaatggat aatgagttta aaagtgaaaa tcaagaaaaa tccatatcta
    24541 ggtcaaaatt aagaaatata tcagatgaag attttaataa tcaagatgag gaaaatattg
    24601 aagcagaaag acttgggtat atgaataata taaatttcta tttaaataaa gcttcttcag
    24661 acttggaaaa agctagtatg gaattgaaaa agctatatga tttagatatg ttggactata
    24721 gagacattga atatgtagat gcaaatatta tttcacatag agattctatc ttagaaattt
    24781 gcaagaatta tacttctgga gatattagta aaataaatga atatattctg aaagaagata
    24841 tagactcaac tacattgatt gaggcaaagt tagtgaataa tgatattctt ttgaaaatgt
    24901 ttttagaaga aaataaaatt gaaaatgaac atttaaattc agccttttca catacttata
    24961 aggcattaga taacatttct ttggcgatgc aaaatatgat tgagtataga catacgtttc
    25021 atatagatga gccaaaagta gaagtatcta atgggattcc agactctcaa caatattatg
    25081 aactaataca aaatcctata ataaatagta tggagtatag ctggaatagt aaatgggata
    25141 cattagaaaa cctattggaa attttaccag atcaaactca aggatttaat tctttaagag
    25201 ttcatttgag aaataatgta agaaaatttg agaagttaag cgataatata tctagtggta
    25261 aaaaaaatac agcaaaaaat ttcttaaagt ataaagagtt attgtatgaa atatcagacc
    25321 aatgtaatca actaaaaagt atgtcttatg agaaaatacc tgttaaatat gataacatgg
    25381 ctttgataac aacttttgac tatatacaaa atgagctttt agcagagata aaatgcagat
    25441 taaaagaatt aaaaccacaa gaaattgata aaacagaagg tattactata aaaatacctt
    25501 tttataaggc tgactacgac atgacaaaag aaggatggcc taaattaaaa gaaaatggtt
    25561 caggggcgga gttaagctta atggtaactg gtgataaaga tggaataaaa gaagtagaag
    25621 ttacagatgg taaaaaaaat attattggtt taggtgttga agaaaattca aattcaaagt
    25681 ataaggttga cgccattgta aactttaatc taaatataga tacaaatgta gttggaaatt
    25741 atgatataaa agataaaatt ttgattaatc atgatataag ttcatacaaa aaagtaaact
    25801 aaatataaga agcaacttag tataaataaa taataaaata attttattat ttactactaa
    25861 aaaaataagt aaactaatag aagatatgga gagtaaatat tatgtatatt tatgatttat
    25921 tagagcaagg tataagacta aacgcatcag atatacacat aaccgttgga acaaatcctg
    25981 tagctagagt aaaaggtgga tttgtaaaac taagtgaaca aatattgaca tcagaagtaa
    26041 caatgcaaat ggcaaaagac atagcggggg aatctatgtt taaagtaata gaagaacatg
    26101 gagaagctga tttttcagct tctctaaaaa caggagaaag atttagggta aatgcatata
    26161 ggcaaaaagg aaattacgca atagcaataa ggactataac agcagaaatt ccaacatttg
    26221 aaaaattagg ccttccagag agtataaaat cttttactga aaagcataaa ggattagttt
    26281 tagttacagg tccaacagga agtggtaaaa gtacaacact tgcaagtatg ataaatataa
    26341 taaatgaaaa acaacaaaaa catataatta cacttgaaga cccgatagaa tatgtacatc
    26401 atcataaaca aagtttagta aatcaaagag aagtaggaac tgatacagaa agttttcatt
    26461 cagcccttag agctattttg cgtcaggacc cagatgtaat actaatagga gagatgagag
    26521 accctgaaac tgtttcaata gcattaacag cagcagaaac aggtcattta gtattttcaa
    26581 cactacatac agtgggggca gctaagacaa ttgatagaat agtagacatg tttcaaccat
    26641 cccaacagca acaaataaaa acacagttat ctacagtttg tgaaggtgtg gtatcacagc
    26701 agttacttcc tacagcagat gggaaaggca gaatagcagc aattgaattg atgtttgcta
    26761 ctccagcaat aaaaaacctg ataagggaag gcaaaacata tcaaattcca aacatgatac
    26821 aaactggagt taaagtaggt atgaaaacta tggaccaaga tttaatggaa ctttataaaa
    26881 atggaaagat tacaaaagat atggcactta gtaggtgtac tgaccaagag tttatgacaa
    26941 gaatgatagg tggagtaaac tacaatggat attataatcg ttaatctata tgtatttata
    27001 gtgggtatag tgtttggaag tttctttaac gtctgtattt ttaggattcc agaaggtata
    27061 tctatttcta gacctccttc tcattgtatg agttgtaata ctagactaaa gccaatagac
    27121 ttaataccag tgttaagcta tttaatgagt ggcagaaagt gtagatattg ttatgagaaa
    27181 atttctagta gatatgctat agtggaatta attacaggac ttttattttt aacagtattt
    27241 atgttatatg gaatatctac atcaactatt tattatttgg ttcttgtgtc tttactgatt
    27301 ataattacat ttatagatat tgaccacttt ataataccag ataaaatatt ggtttttgga
    27361 gctatatttt ctatgatttt taacctttta tttaaagaaa ttccagttaa ggattcaatt
    27421 ctaggtgttt ttatttgtgg aggaagtgtc tggattatag ttcttttgat tgaatttgta
    27481 ataaaaaaag agtgtatggg tggaggagat atcaaactat ttgcaatgct aggtttttac
    27541 atgggtgtta aaaatggtct tttgacagcc ttactaagcg tttatgtggg agcagtctat
    27601 ggaatctgtg taataattat aagtagaata aaaagaaagg aatataattc tgttatacca
    27661 tatggacctt ttatatctat tggagcatta ataacaatct tatgtggaaa gcaattactt
    27721 gaactttatt tttctatggt tatatagatg atagtcaagt taaataaagt tttaatttaa
    27781 aaaaatgaat taaagtattt aataaggaca atattattat taaccctatt actaaagtgc
    27841 tacaaactat ggagtatata ataataattt ccttattttt tgcacatttg ataaataaat
    27901 ctctatgtgt tttttgtgat agagaaaaga aaaatttata caatacgttt tttatttaca
    27961 taataactgt agttttatct gtattaatat atctaaaatt tgggattagt gtagaatgtg
    28021 tacaatatat ggttttaatt ccatttttaa taattatatc cattgtagat tactacacaa
    28081 tatatgtata tgatataaca atagttagtg gtataatagt tcaaggtata atttttttgg
    28141 tctttttcaa tatagttcaa tttgattata taaatcatat atttggacta ttgataggct
    28201 ttattatacc ttatattatt gctaaactaa caaaaggatt aggctatgga gatgtaggtc
    28261 tctatagctt atgttgcttt gcattgggaa ataattatgg gttttatgtg atagttttat
    28321 catttatttt agcgtttttg ttttttatta ttttatacat aactaaaata tttaaagatg
    28381 tagatataag aaagcaaatt ccttttacac cttttatttc cttgtcaaca attttaataa
    28441 tatttacaga gtataatata tttaattttt attataattt gttatacaaa tttatataac
    28501 aggaggtaag aaatgtacgt agtagtaggg ttaggtaatc caggaaagaa gtatgagaag
    28561 actagacata acgttggatt tgatgttata gatattcttg ctaaagaata caatattagt
    28621 gttactaaaa taaagcataa agctttgatt ggtgaaggga gagtaggtac tgaaaaagta
    28681 ttattagtta aaccacaaac ttatatgaat ttaagtggag aaacattaat agatatatat
    28741 aaatattata aggttgattt aagtaatata gtagtagtat atgatgatat agatttagaa
    28801 gttggaaaga tacgtataag aaaaaaagga agtggaggaa ctcataatgg catgaaatct
    28861 ataactaagt gcctaggttc aaatgacttt ccaagagtga gagtaggtgt atctaaacca
    28921 gaagctggtc aagacttagc agactttgta ctttctagat ttagaaaaga agaatctgat
    28981 aacataaatg aagcattaga aaaagcggca gatgctattg actcaataat aagagaaaat
    29041 atagatatgt ctatgaacaa atataatgga tagtgagagg gagatttaaa tgaatgatgt
    29101 ttttttatat cctttgcaaa attcaaaaga gtataaagat ataataaact gtataaaaaa
    29161 tacaaaaggc tcattattgg ttaatggact tttaccagta caaaaacctc atatatcgta
    29221 ttctatattt aatgatttat cacgacaaat gatttttata acaagtagtg atttagaagc
    29281 aaagaaagta tacgaagatt taagttttta tatggaagat aaagttgaat atttaggttt
    29341 tcaagatatt tatttttatc acttggatgc aaaggacaga aacgaagaag cgaagaaatt
    29401 aaaagtattg ttaaaactag caaataaaga aaaaatcatt ttagtaactt ctattgaggc
    29461 agttttaaga aaatacatac caaaacaagt tttgctcgat agtgtatctc attataaggt
    29521 gggagatagc ttagacttag aaaagttaac tgagaaatta gtttcattgg gatatgaaag
    29581 agtttcaaaa atagaggggt ttggtcaatt tagtataaga ggtggaatta ttgatgtatt
    29641 ttcacttgaa tatacaaatc caattcgtat ggaattattt gatgatgaga tagattccat
    29701 acgaactttt gacgtatatt cacaaaaatc aattgataaa ttgcaacaat tctctataac
    29761 tccatctagg gaatttattt atcccgaaaa aactacagat gcattagtta aattaaaaaa
    29821 agaaacaact aaaaatactg atgaaggtac atttcaaaat atagattata tatcaagtaa
    29881 gacttatttt gaaggtgtag agaactatat agattatatt tatccagaag aaaacaaaag
    29941 tatatttact tatttagcag atgatgcaat agtatttatt aatgatataa cgagacttaa
    30001 agaaagatgc gaaaattata taaatgagtt tagagaaaat tataaattaa atcttgaaag
    30061 aggtctggct attaaaaacc aaggtaaatt gttatatcac tatacagatt tagagtactt
    30121 agtaaaagac aagtctgtgg tattaaactc gctattgcca aagtctataa ataacttttc
    30181 tataaaatct ataataaatt ttgaaagtag agaagttcct acatttaatg gaaaggtaga
    30241 tttgcttgta gaagaattaa atcggctgaa atataatgga tacaaaataa ttttagcaac
    30301 aaatacctta gaaagagcaa ataaattagg aaaagattta ttggacaaag gtttagaaac
    30361 gaccatttct aaagatagag atattgaaat taaatcttca caagttataa ttgtacctgc
    30421 acacataaac agtggttttc agtataaatc tataaagttt gttgttataa cagataacga
    30481 aatgataggt gtttataaaa gagcgtctaa gactagcaat aaaaaagtta aaaagggaaa
    30541 gaaaatagaa tcctttttag atttaagtgt tggagattat gtagtccatg aaaatagtgg
    30601 tgtaggtaga tacacaggaa ttgaacaaat tacagtcaat gctattaaaa aagattatat
    30661 gaaaatagta tatcaaggtg gagataatct atatgtgcca atagaccaga tggacaaagt
    30721 acaaaaatat attggtgcag aagtagaaaa agttaagtta aataagcttg gaacaaatga
    30781 gtggacgaaa gcaaaagcaa aagttaaaaa agaaattgaa gatatgacaa aagacttgat
    30841 tgagctttat gcaaaacgtg aaaaaataca agggtataag ttttcaaaag atacaccttg
    30901 gcaggcagag tttgaatcgt tgtttccata tcaagaaaca gaagaccaat taaaggctat
    30961 agaagaaact aaaaaagata tggaatcaag taaggtaatg gataggctag tatgtggaga
    31021 tgttggttat ggaaaaacag aggtagctat tagaagtatt tttaaagctt gtatggacca
    31081 aaaacaagtt gctgtattgg ttccaacaac aatattggcc caacaacatt ataatacatt
    31141 caaagagagg tttgaaaatt atcctttgag agtagaagtt ttaagtaggt ttaaaacacc
    31201 aaaacaacaa aaacagatta ttgaagatgc taaaaaagga ttagtagata ttttaattgg
    31261 aacacataga ataatatcca aagatataaa tttaccaaac ttaggtcttg tagtcattga
    31321 tgaagaacaa agatttggag taaaacataa agaagcttta aagaaaatta aatctacagt
    31381 agatgtactt acactttcag caactcctat accaagaaca ttacatatgt ctttaagtgg
    31441 aataagagac atgagtgtta tagaagaacc tccacaagaa agacatcctg taataacata
    31501 tgttacagag agtaaggaaa gcgtaattca agatgagata gagagagaat taagtagagg
    31561 tggacaggta ttttttgtat acaatagagt tgaacacatt gaagaaatgg caagtatgat
    31621 tcaaaaactc gtaccagatg ctagagtggc tgttgcacat ggaagaatga cttctaaatc
    31681 attagaaaat ataatcctag gatttttaaa taaagattat gatgtactag tctgtactac
    31741 aataattgaa actggtatgg acatatcaaa tgcaaataca atgattatat atgatgcaga
    31801 taaaatggga ttggctcaac tttaccaatt gagaggtagg gttggaagaa gttcaagaca
    31861 aggatatgca tatcttttgt atgaaaaaga taaaacttta agtgagatag cggagaagag
    31921 attaaaagct atcagagaat ttacagagtt tggttctggt tttaaaatag caatgagaga
    31981 ccttgaaata agaggagcag gaaatatttt aggttctcaa caacatggtc atatggcagt
    32041 tataggttat gatttgtacg ttaaaatgct taatgatgca attaaaaaag tcaaaggtga
    32101 acctattgtg gaggaaattg atgttgaaat agatttaagt gtaaatgcat acattccaga
    32161 taattatatc aaagatgagt tgattaaaat tgaaatgtat aagaaaatag cttctattga
    32221 aaacaaagaa gacatgttgg atatacaaga agaacttgag gatagattct ctgatatacc
    32281 taaaccagtc cagacacttc ttacaatagc ttatataaaa tcgttatgta agatattaaa
    32341 aatagaaaag ataagacaat taaaagatga aatattatta gttccaataa caaagtatag
    32401 aactaaacag aaaataggtt ataatatagt tacagaatta gaagaattat tagaaaagat
    32461 gtgcaaggta aaataacttg taaatttaag aagggatgtt ttaaatttga aaaaagtaat
    32521 aacattggta atagctatga ttttggtagt atctgtgact gcttgcagtt ctagtaaagg
    32581 agaaactgtg gcaacagttg aaggtacaaa gatatcatca gatgaattta aaaaaactat
    32641 agctctttat aaagattcta tggaacaaac ttatggaaaa gatatatggg ataaggaagt
    32701 tgaaaagggt gtaaaataca aggacaagtt taaagattta attcttgacc aacttataac
    32761 tacagaagta atatattctc aagctaaaaa agataactta cttcctaaaa aagaggatgt
    32821 tgaaaaaagc tttaaagaat taaaagacgc tatgggtaaa gatgaaaaat ataaagaaca
    32881 acttaagaaa ttaggtatag atgatgagtt tttaagagac caacaagaga aagatttagc
    32941 aatgcaaaac tatcaaagta actttgctaa aaagactaaa atatcagatg aagaaatgaa
    33001 aaaatactat gatacacata aagatgaatt taaaaaagat gaggtagaag cttctcatat
    33061 tttattgaaa acagtagatg ataataacaa gccactttca gataaggaaa aagctgaagc
    33121 taagaaaaag gctgaagagg ctcttaaaga agttaaatca ggtgaggatt ttgcaaaagt
    33181 agcaaaaaaa tattcacaag atacttcagc aagtgatggt ggtaaattag gattcttttc
    33241 aaggggtcaa atggttgctg aatttgaaga tgctgctttt tctatgaaaa agggtgaagt
    33301 atctgattta gttgaaactc aatatggata ccacataatt aaagtgacag acagaataaa
    33361 tgagcaaaca tcatttgaag atgctaaaga aactataaaa gaccaactgt tgaaaaataa
    33421 atatcaagaa caaatagaaa aattaactaa agaagctaag gtagaaaaag atgaaaaagt
    33481 aataaataaa atcacaatct aagacttaaa aaaagagtaa tatgcttaaa aaagctatat
    33541 ctggataaga tatagctttt tttgtatatt tttcttaaca atggtaaaaa taaacttaaa
    33601 gtaatttatg gagggattaa aaaatatgaa agcaacaggt atagttagaa gaatagatga
    33661 tcttggaagg gtagttatcc caaaagagat aagaaagaca ttaagaatta gagaaggaga
    33721 ccctttagag atattcacag caaaagatgg agaagtcata cttaaaaaat actctccaat
    33781 aggtgaatta aatgaatttt cgcaagaata tgctgaaaca ttagctgaag tactaggata
    33841 tggagtgttg ataacagact tagattctat aatagctgtg tctaagcttc ctaaaaaaga
    33901 ttataaagaa aaaagtataa gtgatgagtt agaagcaata ataagtgaaa gaacagtaaa
    33961 atattcaaaa gataaaaaca ttttaccatt attcaaggaa gaccaaaaag aatatactag
    34021 tcaagttatc atgcctataa ttagtgcatc tggtgactgt ataggttcaa ttgtagttgt
    34081 ttcaaaggat aaagatacaa ttgcagaaag tgaagaaaaa tctttaaaaa ttgctgctaa
    34141 ctttttagga aaacaagttc aataatttta tcataaagac cttttacatg aaggtctttt
    34201 gaagtttttg tgtaaaatat tgaatatagc attaaaaata tggtatatta aagtttgtta
    34261 tataaacagt atggaggtta tgttatgaac aataacaaaa ataaagactc ctttctaaaa
    34321 ggagccctta tacttgggct agcaggtgta atcgtaaaaa taatgggtgg attttttagg
    34381 attccccttg gaaacatgat tggttcagaa ggaatgggat actatcaggc tgcttatcct
    34441 gtgtatactc tatttttaac attagctact gctggatttc caacagctgt ggcaaaattg
    34501 gtttcggaaa aagttgcaat tggaaatttt aaaggtgcaa ataagatatt taaggtatca
    34561 catacagttt tattcattac cggtataata tcattttgta tattattttt tggagccgac
    34621 tacatagtaa caaacgtaat gaaaaatcca ggtgcgttat actctatgaa agccattgct
    34681 ccagcattac tatttgttcc tgcaatgtct gcatatagag gatattttca agggagacaa
    34741 gatatgacta agattgctgt gtctcaagtt gcagagcaat tttttagagt tgtattaggt
    34801 cttactttgg cttacttgct tatgaagagt ctaggtcaga cttttggtgc agcaggtgct
    34861 atatcagggg ctacaatagg ttcaatagca tctatgcttt atttggtgtt tgcttatatg
    34921 ctagggagaa aagagcgtag agcagagata gatgcaagcc aaagatttaa agatgaaaga
    34981 gtgtcttata tttttaaaaa gcttttaact gttgccattc ctataacaat aggagcatca
    35041 gtaatgccac ttgtaaatat gatagataat gttattgtaa taagaagact tatggaagca
    35101 ggatttacat acaaagtggc aaattcaatg tttggtcaat taacaggtat ggcaatggcc
    35161 acaataaatt taccagcagt cataacaact gcaatgagta tgagcttagt tccagcaatt
    35221 tctaaagcat atgcattagg aaataagtca aaagctagaa aagatactaa gagtgcagtt
    35281 aaggtaactt tacttatagt attgccttgt gcgtttggta tggcttcttt agcaatacca
    35341 ataatgggtc ttttatttcc acatgaacca tcttctgtag gaactatttt gtttactctt
    35401 actccatgtg tattattttt aggattaata cagacattga ctggtataat tcagggaatg
    35461 ggaaaaccaa tagtacctgt aattgcttta tgtgttggta tgctatgtaa aattgtaata
    35521 agttatactt taacaggtat accagatatt aatgtactgg gttcagcatt tggtacagtt
    35581 acagcatact ttgtggcagc catgattaac ttactatatg ttaaaaaaca catgaatgta
    35641 aacttttcta aaaaagaatt tataataaag ccatttataa cagttatgac aatgtttata
    35701 atggttaagt tgagttatgg tgctttagtt ggattcttag gaaacagcat atctacaata
    35761 attgcaatat gtgttggtgg aattgtatat gtagtagtaa tacttggaat aggtggaatt
    35821 aaaaaagaag agattctaac tatgccaaaa ggtgataaat tatataaatt gttaaaaaaa
    35881 gttaaactta taagataata taggaatata tatgcaaata aaggaaatac tatacaaata
    35941 gtaaatctcc ttatttgtat aataatataa aaataaagtg aaatttgcta ataggagtga
    36001 tgttatgggt aagataagta ttgtaggtct tggaccagga gactattctc ttataagcca
    36061 aggtgtgtta gaatctttaa taaatagccc taaagttttt tttagaacag agaaacatcc
    36121 aataattgat aaacttaaag aaactataca atacacttct ttagactact tttatgaaaa
    36181 agaggaaaat tttgataatg tttatttgaa aatatctgaa tttatcatag aagaatcagc
    36241 aaagggtgac ctagtgtatg ctgttcctgg tcatccaaga gttgcagaga agactgtaag
    36301 tataatagag agattagcca atgaaaagca catagcagta gaaatattgc cttctatgag
    36361 ctttgtagac gctatgttta actatttggc tatagaccca tctgagggat ttaaactggt
    36421 agatgctttt gaaatagaaa attcatatat agacttggat acaagcatga taataactca
    36481 ggtatatgat aaatttatag catctaatgt aaagttaaaa cttatggaac attatgacta
    36541 taatcaagaa gtatgtatag taaatggagc aggtgtcaaa gatttagaaa gtaaaaagtt
    36601 tataaagctt tgtgaattag acagaaatga aaactttttt gactatttaa ctagcttata
    36661 tatacccaaa agttctaaaa agatgtataa tacagtacat gacttagaaa taatagttaa
    36721 tacacttaga agtccatctg gatgtgaatg ggataaaaaa caaacacatc aatcattaaa
    36781 aaattctgtt atagaggaag catatgaact ttgtaatgca attgataata atgatataga
    36841 tgaaatggta gaagagttag gtgatgttct tttacaggtt atattccatt gtcagatagg
    36901 aaatgaggag ggctactttg atttaaaaga agtagtaaat ggcatatgta aaaaattaat
    36961 tcatagacat cctcatatat ttagtggtgc aaaattagat atgtctaagt ttgagaaaac
    37021 ttgggaagaa ttaaagaaag aagagaaagg cgaaagtact ataacagatg gccttagaag
    37081 gataccaaaa catttaccag ctcttatgaa ggctagtaaa gttcaacata aagcagcact
    37141 tgtcggcttt gattgggaca atgtagaaga tgtatttaaa aaaattgaag aagaatatca
    37201 ggaattactt gacgaacaca gggtcgggaa tataaaatac ataaaggaag agctaggcga
    37261 tttattattt tcgatagtaa atctagctag atttttagac atagactcag gagaagcctt
    37321 gaactgtact acagaaaaat tcataaatag atttgacttt atagaaaaaa gtgcaatgaa
    37381 cttgaataaa aaattagaag acatgacact tgaagagatg gatgagtttt ggaatcaggc
    37441 taaaaaacaa taaaattgtg gaaggaaatt tgtcaaaaat ctagaacttt tatcacaaag
    37501 atagaagtct atattataaa catttttagg aggtaaatat cgtgaataaa gctgaattag
    37561 tatcaaagat ggcagaaaaa agtggattaa caaagaagga agcagaagct gcgttaaacg
    37621 catttatgag ttctgttcaa gatgcactag taaataatga aaaagttcaa ttagttggat
    37681 ttggaacatt tgagacaaga gaaagagctg ctagacaagg aagaaatcca agagatccag
    37741 agcaagttat agatatacca gcttctaaag caccagtttt caaagctgga aaaggattaa
    37801 aggatataat aaatggataa ttagaaatct tgtgaaaaag tgtggataaa tttgccacac
    37861 tttttctata gaaccaagga aggtgataat tatgagatta gataaatttt taaaagtatc
    37921 taggattata aaaagaagaa ctgttgctaa agatgcatgt gataaaggga ttgtcactat
    37981 aaatggaaaa gttgctaaat cttcttcaga ggtgaatatt ggagatacat tagaaataca
    38041 atttggagag aagaagatga agtttaagat aaatgaagta aaagagcatg tattaaaaaa
    38101 tgatgcaaaa gaaatgtacg aaattttaga ataaaattaa tagtcctcta gtatattaat
    38161 agtagtatta ttgaaatatt tactgggggg atattatgga acagaacata actttgaaag
    38221 atagatcaaa gctggttatt tcgggagtcg agcacatata ctcttttaat gacaaaagag
    38281 tagagctcaa aacttctgta ggcgagatgg taattgaagg cgaaaaccta gatatgagca
    38341 agctaagtat agacgaaaat ataattagca tagatggaac tataaattct atggtatatg
    38401 caaagcctaa aaagccacaa gagagtttct tgaaaaaggt atttagatag atgatacctt
    38461 ttactcaaga tgtaagcatt ttttatgcta ctatttatgg tggaatactc ataggggtgt
    38521 tgtttgattt ttatagaggg ctaagaggga attttaaatt catcaattat tttgctataa
    38581 tctttgatgt attgttttgg tttctagcta cggttataat attcgtaaca ataaacctta
    38641 cagaattttt tgatttaaga tattatcatt ttgtggcttt gtttattggt tttattttat
    38701 actataacac cataagtaaa attgttttat caattatcaa taaaataatt cgttttgtga
    38761 gaaattcttt taaaaaagtt acacactaca tagtcagttt tttaaataat ttgtattacg
    38821 ttataatcta ttcactacac ctactatttg atattatatt ttatatacca aacatattta
    38881 tagcaacaag aaaaagtata aaaagaaaat caaataaaaa attaaagaac aagaaaaaat
    38941 caaaacctaa gaagaaaaaa aataaaacta aaaaaagggt gtaggaaaaa tgaacttaag
    39001 aaaaaaattt tcaggacaga ttattgttat cagtttattt ttaggaatat caatttttag
    39061 tatgatgaca ggatttgtat ttgagtatac aaaagctaag gaatataaaa aagaaatagc
    39121 aagtctaaat aaacaattaa aaaagactga aatacaaata aacagcttaa agaaagatga
    39181 aaaatcatat gaaggagact tagaggatat agcaagaaaa agattgaata tggtaaaacc
    39241 aaatgaaaca gtttatgtag acataaatcg ttgattaaaa cttgctaata agtttacaga
    39301 tgtattgatt ttaaaattta agatttaata aaaaagtcat tcagttaagg atggcttttt
    39361 gtttttattg aggagggaat ttcataatga aagtaggagc tatagatatt gggacaaact
    39421 caatgagatt attgatagca gattatagag atgggaattt tataaacaga aaaaaatttg
    39481 ttaatacaac tagaattggt tatggtgtgg atagtaaagg atatataaaa gaagatgcta
    39541 tcaaaagaaa tataaaagca ttaaaagaat tttctgataa gtgtaatgat gaaaagtgtg
    39601 aaaaaattta ttgtatgggg acatctgctc ttagagacag taaaaatgga gattactttg
    39661 ttaaattagc taaagaagaa actggaatag atgttgatat agttacggga atagaagaat
    39721 caaatttggg ctttaagggt gttttagaag gaatacaagg tgatgatact attattgtta
    39781 tagatattgg aggaggttca acagaattta ttgtaggtga ttcagaagga attatattta
    39841 ataaaagtga gaatgtaggt gctcttagga tgacagagag gtttttgaag cacgatatta
    39901 ttgaagatga agaatttgaa gctatgagtt catttatata tggtgagatt gataaaacgt
    39961 taaatcatat aaaagacaga ggattaaaaa ctcttatagg tataggaggt acgattacat
    40021 cattatcagc aatgaatcaa gaattagaga tttactctat ggaaaaaata cataatagta
    40081 aaatacacaa aaaagaaata gaagaaattc tacaaaaatt aataaagatg acattaagtg
    40141 ataaaaagaa cttaaaaggg ctacaagaaa aacgtgcgga tattataact gctggtgtaa
    40201 aaatattgaa tataataatg gaaaaattag aaatagaaga aataaccgta agtgaatacg
    40261 ataatttgga aggcttaata tgtcaaaagt caaaaaaaat gtattaatat tttttgattt
    40321 ttaaaaccga ttatgacaaa aaaaattact cctctttgtt acaattctta atataaacaa
    40381 aagaggaggt ttttttatgc aaagaagtgt agcagttgtg aagaataaga ctgtaaaacc
    40441 aaatgtcata aaattaataa gcggatatat tgattttact acggcggcat tcgctgtgat
    40501 gggattttta cttagtaggt ctattatagt agactctgta gctcctttag gaattgcctt
    40561 ttttatatgt gtatcaaaga ttgataagta caagatacct gtattcatat caacattatt
    40621 aggaatattg ttatcatcta atgattttgg aaatgtagca aaatatgctg tgtgccttac
    40681 aatatttatg gctatgaata aaaaattaaa attatcagat tcaattgcta aggttgcatt
    40741 tataggtgca ataatactac ttccaatctc catgggacaa gctttctttt caaataggta
    40801 tgtatatggt cttcttatag caggagtaga agcaataata atgtttatat ctatatatgt
    40861 gttttcttat gggataggtt taatggccaa tgtcaacaat aggatgtctg tacggacaga
    40921 agaagccata tcaataagtt tactgctaac ctttagcata atgggaatag gagatatatc
    40981 tctgtttgga atatcaataa ggaccatttt atcgaccgta ttgatattgc tgacctcaat
    41041 agtaggtgga gctaccatgg gagcatctag tggtgtaatt attggtgtag catccatttt
    41101 aaataatatg acatcagctg tttatatggg gatatattct ttttcaggat taataagtgg
    41161 agcatttaat aaaataaata aatatttctg tatactagga tatattctaa gttggacaat
    41221 aatataccta tatacttctg gtattacttc taatatgatg caattaagag atatactatt
    41281 aggttgtttg atagtactag tattgccaga gcgattattt aataagattg aaaaattaat
    41341 aaaaagtaat gtggcttcaa atgaaatcgt atacgattat attatgagaa gcaaaaacct
    41401 aactaatagc agattgaaca gtatttataa aacttatgat gaccttgcag atacatttga
    41461 taagataaga gaaaaagaca aagtgctaga ccaaagagac atagctaatg taatagatat
    41521 gattcataat gatgaatgta aaagttgtag tatgagaaga atgtgctggg agtcaagatt
    41581 taatcataca tacactatga tatataacat tcttgaaaaa attgaggaga aaggggagtt
    41641 aagtctaaac gatataccta aaaattttag aaaggaatgt atgaaaccag agtcaatagt
    41701 gaaaatatca aatcactatt acaaaatgtt tgtattagat tatgattgga gtgtgaagtt
    41761 ttcagaaagt agaaagttaa tagcaaatca gataagaagt atttcaaaat ccattaaatc
    41821 cttatctcaa gatttagaag gtgacataat gctagatata gaaaaggaaa aaaatatata
    41881 tgaacagtta gaaagatatg atataacagt tgataaagta agttacttaa caaaaagtaa
    41941 cagtgagttt gaaatatcaa tagaaaagaa aacttgccat gatggatgta tgtgtgaaga
    42001 taaaattgta aatataattt cagacctagt aggtgaaaat atgtctgtta gaaaaatagg
    42061 atgtcactgt ttaggaggta aatgcaaggc aacattcgtt aaatctcaaa aatataaggc
    42121 tgttacagaa gtttcagcaa tgtcaaggga tgggcatatc ctttgtggag acaattacac
    42181 atacatggag ataaatgatg gtaaatacat gatggctata agtgatggta tgggaaaagg
    42241 taaaaaagca tatgaagaat cttctgccac tattgatata ctggaaaaaa tgatagatgc
    42301 aaaaattaaa gatgagatag tcattgatac tataaataat atgttgttgt taaaatcttc
    42361 agaagagatg ttttcaactc tggatttggg aattttagat ttaaaacgag gttgtttaga
    42421 aactataaag atgggagcgt gttctactta tataaaaaga gaggatggag aagttgactt
    42481 aatatcatca tcatcattac ctgtgggtat attatcagat gtaaaaattg atagaaaaaa
    42541 tgtaaaagtt aaggaaggag actatgttat aatggtttct gatggaattg tagatgcagg
    42601 aagaaataac aatttaggag acaattggtt aatatacttc ttaaagaata tagagactac
    42661 aaatccaaag gaaatttcaa atttaatatt ggatagagca ttagagttac aagcgttaca
    42721 aatcgaagat gacatgactg ttttagttac gaaaatatgt acaaaataag gtttacaaga
    42781 ttatgtgtat aagatgtgga taacttgtgt ataatatgtt aatagacttt tgaaaaaagt
    42841 atagttatta acaaaaataa catataaatc aacaaaattt gttgataagt atggcagaaa
    42901 tgttaataaa atgtaaaaaa agaaaaaaca aaagctatta tcaaaagaga ttcatgttga
    42961 aataactaaa tttctaagta aagaaaacct gtgaatatgt tgattattat gtggataact
    43021 attaattcga tatttattta tatagtatga ataattgaat aatatatcta aaaaaagcca
    43081 tcctattatt aagtaaactt attgaaagga tggcttttta tgaatagaaa attgtgcaaa
    43141 gcaataatac ctttatttat ttctattttt tgcatgttaa taataagtat aaaagctgac
    43201 caaggatatc aaaagaataa tgacagagca attactgtca tgaataataa tgatactgaa
    43261 gatgatgctg aaaaaaaaca agaaatagac tatgaaaaaa catgttggaa tttgcaatct
    43321 ttgtttaaaa gtgatgacca atggaaaaaa gaacttaaga gttttgataa agatactaaa
    43381 gagttgaaaa attacatagg taaagtaaca aaatcgccaa cacatttgtc ttttgcctta
    43441 aacattaaag aaaaacttga tataagacta aataaacttt ctgcctatgt aaaattaaaa
    43501 caagatacta ataaaaattc gtataagtac ttagatatga atgatagtat gagtaaatcc
    43561 tatgggaatt atatggggat ttgttcagat ttagagttag aaatactaaa attatcaaac
    43621 aaagattaca agaaaattat ttcaaataaa aatataaata ggaaatatgg tgcatattta
    43681 agagatataa gaaggaataa agtacattat ttggatgata aatcagaaga tattctaagc
    43741 aaagtatctt caataagtag tcttccaagc caagtgtatg aattatttag aaatatggat
    43801 agaaaaacta atttgacacc agctcaatat gcgagtgaat tagagtcatc agatagagaa
    43861 aatagaaaaa aagcttatca agatgaactt ataacttaca atgataatat aaatacaatt
    43921 gcaggtttaa ttactggtca agttaaaaag aatatttttt attctcagga aagaggttat
    43981 gagtcttctt tagaaatgta cctggaagct gataatgtag atgaaaaagt atacaataat
    44041 ttgataagga ctgtaaatag taatatggac agtctacaca aatatataga tttaagaaaa
    44101 aaagtattaa aattagataa ggttcattca tatgatatgt ttgtgcctat ggttaaggct
    44161 gttgataata atattaatta tgaaaaggca caaagtatag tatattcagc gttgtcacct
    44221 ttaggaaaag aatacaatga tgtagtatat aaagctttca atgaaaaatg gatagatgtt
    44281 tattcacatg aaaataaagt aagtggtgga tactgcttat ctgtttatga aaatcatcca
    44341 tatatacttt taaattataa taattcatta ggttcagttt cgactttatc acatgagctt
    44401 ggacatgctg tatatgaata cttaagttct aagaatcaga attattttaa ttcaaatccg
    44461 tctatattta ctcacgaagt tgcatctaca acgaatgaag cattacttta cgagatgctt
    44521 ataaaagaag ctaaaggcaa tgatgaaaaa gcttattata taacacagta tttagacttg
    44581 ataaaagata ctctttatac acaaactatg tatgctgaat ttgaaaaaac tatacacgag
    44641 ttagtagaaa aaggaaaaag tgtaaacgca ttagtattaa atgatgtttg gggacaactt
    44701 cttaaaaaat attatggaca aagctatgaa ttagaccaat tatctaaggt cggatgggct
    44761 agaatcccac atttttataa tagtttctat gtctataaat atgctacagg ttgtagtgca
    44821 ggagtaagtt ttgcccaaga catcctaaag aatggttcag ataactatat gagctttctt
    44881 aaaaagggaa gttcagatta tccaataaac ttgttaaaat caagtggcat aaatttaact
    44941 aatacaaaac ccatagaaaa tacaatcaaa aaatttgata gtttagtagc agaacttgaa
    45001 aagctattat aaacattatt gtaatccaca tggtaaatat atcgaattaa tgataaaata
    45061 tactaaaagt ttaaagtcct atcaagatag gactttttat ttattaatta gtgggagatg
    45121 attctatgca agttaaagtt aaaaaggctg taataccagc agcaggttta ggaactagat
    45181 ttttgccagc tacaaaggca caaccaaaag agatgttacc aatagttgat aagccaactc
    45241 tacaatacat aatagaagaa gctgtggcct caggaataga ggaaatacta ataataacag
    45301 gtagaaataa aaaatctatt gaagaccact ttgataaatc tgtagaattg gaacttgatt
    45361 tagagaaaaa aggtaagaaa gaattgttgg aggtagtaca aaacatatct aacatgatta
    45421 acatacatta tataagacaa aaagagccaa aagggttggg agatgcaatt tattgtgcga
    45481 ggcattttat aggtgatgag ccgtttgcag taatgttagg ggatgatatt gtagataatg
    45541 atgtaccttg tctaaaacaa ctaacagatg catatgagga atatagaact acaatattgg
    45601 gtgttcaaaa ggtaaatcaa gaagatacta ataaatatgg gataatagaa gcaaaaaata
    45661 tagaaggtag agtttacaag gtaaaagaca tggtagaaaa gccagagtca ggaaaagctc
    45721 cttcaaatat tgctatttta ggtagatata taataactcc tgaaatcttt gatattctta
    45781 aggatttacc accaggtaaa ggtggagagg tacaacttac agatgcttta aaaatattat
    45841 ctaagaaaga agctatgtat gcatacaatt ttgaaggcaa gagatatgat gttggggaca
    45901 agttaggatt tttagaagct actgttgact ttgctctaaa gaaagaagac cttaaggaag
    45961 attttataaa atatttgaaa catgtatgta gtgaatttga taaaaataac aatgtgttat
    46021 acaataatac agatgaaaaa atagatttaa ttgaagtcaa tgaagctaga gaacttgaaa
    46081 ttcaaaaata attaatgaaa ggtgatgtat ggtggcaaaa tcaagtcaga aaagaattaa
    46141 tagaataata gctgttggga ttgtgggaat cctcgtaata acgagtataa cagctgtttt
    46201 gagtatgatt gcaactatgt aataagataa atgacagcag ttgatataca taaatataaa
    46261 aaccatatat aacttgatgt agtcaaaaac ttacaatatt ttagttatat atggttttta
    46321 gaatttataa atgtaaaaaa aaatttttat aatagttgca tattttaaag gaaaatatta
    46381 ttataagata taaatatagt gataaattta taaaaacgtg ctaagattgg acttgaaata
    46441 cagtaatata gtaggtaata atgttttaaa ttttatttat tgaccataat atataaataa
    46501 tgatattatt agacttaact gtttaaataa aacttttctt tttcatgcct tcaatttcca
    46561 aaataagtat gtatataaat gaatatatag atgaggagga tttttttgat tttagatgat
    46621 aaagtattag aaggattagg tagtaagacc ttagttgagc tacgagagat agctaaagaa
    46681 ttaaaaataa aatcaattac tacatataag aaaaatgagt taattgagat tataaattcc
    46741 aaaagtaaaa atgaggaaaa agtaaacgaa gagactaaag aaaaaaatga agatattaaa
    46801 gaagacataa agaatagaaa agaaaatata gagcgtgaaa gtatagaaaa agaagttgaa
    46861 tataagagcc ctaccaaatc atacaacaag aaagaaatag atacccaaaa tatttcccaa
    46921 aaccaagaac atagaaatca aagaaatgac tataataaga caacgagtgt aaatgatagt
    46981 aataagtcat caaatatatc taatagaatg gtcagaaata ataataaaaa ctattatatg
    47041 cctaaacaag ttgatgaatc taagatagta gatgaattta acacatcaaa agaggatgaa
    47101 gtagtaggtg tacttgaaat tctgccagat ggatttggtt ttttaagagg ttcaaattat
    47161 ttgtctactg aaggagatgt ttacgtttca ccatctcaaa taagaagatt taatatgaaa
    47221 acaggagaca agattaaggg aataactaga catccaaaaa gtggagagaa atttagagca
    47281 cttttatatg ttcaaaaaat aaatgatgaa aaccctgaca cagctataca aagaaatgcg
    47341 tttgagacat taacaccaat atttccagaa gaacgactta cactggaaac taatagaaat
    47401 gaaatagcta caagaattat agatttaatt tcacctatag gaaaaggaca aagaggattg
    47461 atagtagcac ctccaaaagc tgggaaaaca gttcttttaa aaagcgttgc aaatagcata
    47521 gctaaaaatc atccaaatgt tgaacttata gtactcctta tagatgaaag accagaggaa
    47581 gttactgata tgaaagaatc tattgaagga gacgttatat attccacttt tgaccaagta
    47641 tcaagtcatc atgtcaaggt tgcagagatg gtgttaaata gagctcaaag attagttgaa
    47701 catggtaaag atgtagtaat tttactagat agtataacca gattggcaag ggcatataac
    47761 cttacaatat ctcctacagg tagaacttta tctggaggta ttgaccctgg tgcacttcat
    47821 ggacctaaaa agttctttgg tgcagcaagg aatattagac agggtggttc tttgacaata
    47881 ttaggaacag cattggttga aactggttct aggatggatg atgtaatatt tgaggaattt
    47941 aaaggtacag gaaatatgga gctacattta gatagaaaat tagcagaaaa aagaatattc
    48001 cctgcaatag atatttacaa gtctggtaca agaagagatg atttattgct tgatgatgaa
    48061 gaaaagactg ctctttggag attaagaaga gaaatgagta ataattcagt aatggaaatt
    48121 acagataaag tcattgaact tataaagaga actaagaaca ataaagagtt tgtaaaatca
    48181 ataaagaatt tgtaaaatca ataatacaaa tgcaaatatg gattatataa actaatagaa
    48241 aaatcacttg aatggcataa taagttgtgc tataataatg tagttgataa tgtaaaaaga
    48301 aagttaatat aattaatttt tttaaataaa gagaagaggt gactataatg caaaaggaaa
    48361 tacaaccaaa atacaatcca gttgaagtgc gttgtgcttg tgggaacaca tttgttgctg
    48421 gttcaactaa ggacgaaata aaagttgaaa tatgttcaga gtgccatcca ttctatacag
    48481 gaaaacaaaa gaacatagaa aaaggtggaa gaatcgacaa attcaagaaa agattcaaaa
    48541 tggactaatt tttataaaag tggagttgga ggaagccaac tcctatttta ttacaaaaaa
    48601 acattcatat tctactttaa attgtctttt tgtattataa taataaatat gtaaaattga
    48661 gtattctaaa tacaataaaa tgaggtgagt ggatggcaaa acaagctgta ggtgggcaag
    48721 ctgttattga aggtgtaatg atgcagtcaa aagacaaaag agcggtagct gttagaaaaa
    48781 gtgacggaga aatagcacta aaagaagata gaataaaaag ttgggtaaga gataagaata
    48841 tagataagat accttttgtt agaggttctt ttgttatgat agatacaatg attcaaggca
    48901 taaaaagctt aaacttttct tcagagtttt ttatggaaga agctgaagag gataaatttg
    48961 atttatttat aaagaaaatt tttaaagata aggcaaatga tataattata atattttcat
    49021 tggtcatagc aatgttactt tctgctggat tattcatttt tatacctaca ttggtaggag
    49081 gagctttttc taaagtaatg cctaatgatt ttatgcttaa tttaatagaa ggaatcataa
    49141 ggatagctat attatttgca tatatagtat tgatttcacg aagtaaggat atagaaagag
    49201 tctttcaata tcatggagca gaacataaat ctatatattg ctatgaaaat gatttagaac
    49261 ttacagtaga gaatgctaga aaatttaaaa gattacatcc tagatgtggt acaaatttcc
    49321 tatttatagt tatggctgta tcaataatat tgtttgcatt ttttggttgg ccaaatccaa
    49381 ttcttagaat atttatgaga attatatgtg tgcctatagt agcaggttta tcttatgagg
    49441 taattagagt tcttgggaaa tatgataatg gatttactaa aattattgca tatccaggaa
    49501 tgatgttgca atattttaca acaaaagagc ctgatgatga gcaattagag gttgctttgg
    49561 aagcattgaa agcagtagta gattagagaa aaggatattt gttatgacga taaaagatat
    49621 aattattaaa tattcagata aactaaaaga tataagtgat actccaagat tagatacaga
    49681 gttattgtta caaaaaacat tgggtgttga tagattatat attcacttaa atctgaataa
    49741 agagctaact gaagaacaaa aaacaaaatt tatgggtttt gcagaagaaa gattaaatgg
    49801 aagaccaata gcatatatag ttgaaaatag ggagtttatg ggattagatt tttttgttaa
    49861 ggaaggcgta ttgattccaa gaccagatac agaaacttta gtagaagaaa taattgaaat
    49921 atgtagagaa aaaaaagatg taagtatatt agatatagga actggttctg gagctattac
    49981 aataagttta gcaaagtaca ttgaaaattc taaaatcatg tcttttgata tatcagagac
    50041 agcattagaa atagctaaaa aaaatgctat tataaatgaa gttggtgaaa aaataaagta
    50101 tattaattct gatttattta cagcaataag tgatagtaac ataaaattcg atatcatagt
    50161 ttcaaatcca ccatatatta aaaaacaaga tatagaaact ctgcacaaac aagttaaaga
    50221 ttatgaacca tataatgctc ttgaaggtgg agaagatgga ttagactttt atagaagaat
    50281 aacagaacaa ggtaagaaat atttaaataa gtgtggaata ttagcatatg aagttggtca
    50341 taatcaagct gaagatgtaa taaatattat gaaaagtaat ggatataaaa aaatatacac
    50401 aaagaaggat attcaaggca ttgatagagt tgttataggt tacaatatat gatataatca
    50461 acaattgata tgttacattt aatcgaggtg aaagtatgtt aaaaaaatta gaggtattgg
    50521 aagatacata taaagactta agtgaaaaaa taggtgatcc ggatgtaata aatgatcaaa
    50581 aagtttggca gaaatatata aaagaacatg ctgacttgga acctattgtt atgaaataca
    50641 gagagtataa aagcgtttta gatagtataa aagaatcaaa agaaatttta caagaagagt
    50701 cagatgaaga attaagagaa ttagctaaaa tggagttagc tgagatggaa gaaaaagtag
    50761 cacctctgga ggaagaaata aaaatactat tattgcctaa agaccctaat gatgataaga
    50821 acgttatagt tgaaattaga ggtggagctg gaggagatga agcagctcta tttgctggtg
    50881 atttatttag aatgtactca aggtatgctg aaagaagaag atggaaaata gaattattga
    50941 gtgcaagtga tactggagtt ggtggatata aagaagtatc ttttatgata aaaggtaaag
    51001 gtgcttattc aagattaaag tatgaatctg gagttcatag agttcaaaga ataccatcaa
    51061 ctgaatctgg tggaagaata cacacatcaa cttctactgt tgcagtatta ccagaagttg
    51121 aagatgtaga ggttgaaata aatccaaatg atttaagaat agatgttttc cgttcttcgg
    51181 gtaatggagg acaaagtgtc aatactactg actctgctgt tagggtaaca catataccta
    51241 caggtgaagt agtatcttgt caagatggaa aatcacaatt aaaaaataaa gagcaagctt
    51301 taaaaatatt aaaagctaga ctttacgata aagcattggc agaacaacac aaagatatag
    51361 cagcagaaag aaaaagccaa gtcggaacag gagacaggtc agaaagaata agaacatata
    51421 atttccctca aggtagaata agtgaccata gaataaattt aactttatat aagttagatg
    51481 cattcttaga tggagatata gatgaaatga tagatgcttt aattactgtt gaccaaacag
    51541 aaaaaatgac tgcaatataa gtaatgtttt aaagaagctg tttcttccta gaaattattt
    51601 tctattttga aacagctatt ttgcatttta ggtcatgtta tcatattcta ttgtatataa
    51661 ttgttatata caagtatagg agggttatat gatactaaaa gtaacaataa ttgggctttt
    51721 ggcaggtgtt atagggacag gattaggagg agtaatatca gccattttta aaagagaggt
    51781 cgataaatat ctaagttttt ttatgggatt atctggtgga ataatgttgg ctgtagtggt
    51841 gtttgattta atgaaagaat ccatggacaa aatggggata atcaatacag ttatatttac
    51901 gtttgttgga gctctcataa ctatgtacat aaaaacaaaa ttagatgtgt ctggaaatat
    51961 ggcatctgga tatcttatat tcataagtat attactacat aatttaccag aaggactagc
    52021 cattggttct tcttttatgt caactgaaag tttaggtatt acacttgcta tagttatagg
    52081 tctacataat ataccagaag ggttagctat ggcattaggc ttagtttgca ataaaatgaa
    52141 actaagtaaa gttattttgt ttactgtaat agctggatta cctatgggtc taggaagttt
    52201 tttaggtgtt tattttggag gagtatttac ttctcttata ggagtatttc ttgctacagc
    52261 aggaggtact atgatgtatg ttgtattgga agaaattttt ccacattcaa aaagtgtgta
    52321 ttgtataatt ggtttcttgt taggtacaat gattgtaaat tatatataaa attaaaatga
    52381 aagcaggcat acaaaagatg aaaactatta taagtaatat tgatataaat aatattgatt
    52441 atgaggaaat aaaaatacaa gcaaaacttc ttagagaagg taaaacagtt atttttccaa
    52501 ctgagacagt atatgggttg ggtgcaaatg cattagatga aaatgcagta aagaagattt
    52561 atgaagctaa aggaagacct agtgacaatc cattgatagt ccatatatat gaaaaagaag
    52621 aagtatatga cttagccaaa gatataagtg ataaagctaa acttgttata gaaaagcttt
    52681 ggccaggacc tataacaatt atattaaata aaaaagatat aattccatac aaaactagtg
    52741 gagggcttga tacagttgca ataaggatgc cttctaatgt tattgccagg gcaataataa
    52801 aagaagctgg tatacctatt gcagctcctt ctgcaaatat ctcaggtcgc ccatctccaa
    52861 caaaagcaaa acatgtatat gaagagatga atggaagagt ggatggaata gttttaggag
    52921 gagacagtaa ttttggattg gagtcaactg tattggattt aacagaagag actcctatga
    52981 tactgagacc aggaagtata actaaagaag ttttagagga acttcttgga gaagtaaagc
    53041 ttgacccatc attaagtaaa aaagaagata atcaaaaagc taaagctcct ggaatgaaat
    53101 ataaacatta ttctccaaat gcagatgtat atataatatc tggaaaaaga gaaaatgtag
    53161 caacaaagat taatgatatg ataaaatcta atagagacaa aggtctaaag acaggtgtaa
    53221 tgtgtattag tagaaaccga gagttttatg atggagaagt aatcgattta ggaaactctc
    53281 ttgaagaagt tgcaagtaat ctgtttgatg ctcttataga aatggacaaa aagggtgttg
    53341 atgtcatata ttcagaagag ttccctaaga ctggagttgg acaagcaatt atgaatagac
    53401 tactgaagtc agcaggatac aaagtgataa aggcttaaga gggagttgta aaatatgaat
    53461 atacttattg tgtgtacagg taatacttgt agaagcccta tggctgaggc tatactaagg
    53521 aaagcaatta aagaatctgg aagaagtatt gaagaatatt ctatatcatc agcaggtata
    53581 tctacagcaa atggaatggg tgcttctgaa aattctatag aagtactaaa agaaatcggt
    53641 atagacttat caaatcatag aagtaaagta attactaaaa aattaattga tgaatcagat
    53701 ataatactaa caatgactaa atctcataaa gaaattttag tacaagcagt tccaaaatgc
    53761 aaagaaaaag tgtatacttt taaagggttt gcaaataaaa atgaggaaga tatatctgac
    53821 ccatttggtg gaaatttgga tatttataga agcactatga gagaaataat gtattctgtc
    53881 aacgaaatag taaaaaaaat atgaaacgga ggataaacaa atgaagatag gattaggctg
    53941 tgaccatgga ggatataatt taaaaaaaga aataataagc tatttagaaa gtaaaggtat
    54001 agagtgtgta gattatggaa ctaataatgc tacagattct gttgattatc ctgtttatgg
    54061 agaaatagtt gcaaattcag ttataaataa agaagtagat tatggaatat tatgttgtgg
    54121 tacagggata ggaatatctt tagctgctaa taaggttcct gggataagat gtgcagttgt
    54181 atcagatgta ttttctgcaa aaatgtcaaa agcacataat gatgcaaata tgttatcatt
    54241 aggcgaaaga gttttaggaa aaggtctagc acttgagata gttgaagcat ggattaatac
    54301 ggattttgaa ggtgatagac atgctagaag agtaaacatg attaaatcaa tagaagaaaa
    54361 acataataaa taataaaaat tacagtttta tcaggaggaa ttatcaaaat gagcaaagtg
    54421 gtagagacaa accatccatt aatacaacat aaattaactt taatgagaga taaaaatact
    54481 ggttctaaag actttagaga gcttttaact gaaattgcaa tgttaatggg gtatgaaata
    54541 acaaaagata taccattaaa agatgtagaa atagaaactc ctattcaaaa aacatcatct
    54601 aaagtagttg caggtaaaaa attagcaata attccaattt taagagcagg tcttggaatg
    54661 gtagatggat tagtgagctt aatgccagca gcaaaagttg gacatgtagg actatataga
    54721 gacccagaaa cattaaaacc agttgaatat tattgtaagc ttcctcaaga cataggagaa
    54781 agagatataa tagtagttga ccctatgctt gcaacagggg gttctgctgt agcagctata
    54841 gatttactta agtctaaagg agctaaaagt ataaaattag ctaatttagt agctgcacca
    54901 gaaggtatag cagaagtgca aaaatatcat gatgatgttg atatatatgt tgcatcagtt
    54961 gacgaaagat taaatgaaca tggatatata attcctggtc ttggtgatgc tggagataga
    55021 ttgtttggaa ctaaataaaa ataaataact ataaatattt attagggact ttcttatata
    55081 gtgtatataa ttttatgtaa gaaggttctt ttttatatat tttttctcaa aataggaatt
    55141 attgaattga taaatttctc gaattgagaa taaaaagttt ttaccaatta aaaaagattg
    55201 cttattttac aaggttttat agaatagtag tctattttaa tgtggcatgt tagttgctta
    55261 cgattaagat tataggtatt aagtgtatta gttaaattga gaattgaata ttaataatat
    55321 tattgggagt ggtaatacat gaagaattgt atagtagtaa gaggggctgg agatttagca
    55381 acagcagtta ttcataaatt acacaacagt ggttttaaag tagttgcgtt agaaatagaa
    55441 gagccattag ctataagaag gcaaatttct ttttgtgagg ctgtttatga aaaaaaagta
    55501 actgttgaag gtgtagaatg tatattttgc aatactgaaa aagaaataga agaagtttta
    55561 tctcaacata aagtagcctt gattatagac ccaaaaggag aaaaattatc taaattaaat
    55621 ccaatcgttt tggttgatgg aatactcgct aagaaaaact taggtacaac taaagatatg
    55681 gcaccactta caatagcttt aggtcctgga ttttgtgcag gagaagatgt agatgttgtt
    55741 atagagacaa tgagaggtca taatctagga agaataataa ctaatggtag agcagaaaaa
    55801 aatacaggag taccaggagc aataaaaggt ttttctaaag agagagttat gcattcacag
    55861 tatgatggca taatggaaaa tgtatgtaag ataggtgata tagttgaaaa gggagaccta
    55921 ctttgctata ttaataatta tgaaaaaaaa catgaagtta gagcaacaat atctggagtc
    55981 cttagaggtt tattaaagga tggagcaaag gtaaacaaaa atttaaaaat actagacata
    56041 gaccctagaa aagaagaagt taagaatgct tttacaatat cagataaagc tagatgtata
    56101 gctggagggg ttttagaagc catactaaac aatgggattt atccccaaaa ttaatttata
    56161 ttagataaag ctattaatat tttattaaga tgatataata aatttaatag attaaagaga
    56221 gcatatgacg ggtatttaaa atttaaatgg taaaaatgct ctttttttat tggacattca
    56281 attaacatgt gtatataata taaaaataag tatttattta ggttgaaatc taataaatat
    56341 ataataccag tacataagca aagaggagaa aagtaaatta tgagaccatc atgggatgaa
    56401 tacttcatgg aaattgcaga agtagtgaaa aagcgttcaa cttgtattag aagacaagta
    56461 ggagctgtaa ttgtaagaga caaacaaata ttaacaactg gatataatgg ttctccaaga
    56521 aatcttgaac actgtgaaaa tattggatgt aagaggcagg aattaaatat tccctcagga
    56581 gaaaggcatg aattatgcag agcattacat gctgaacaaa atgcgataat acaagctgct
    56641 cataatggta ttagtgtaga tggggcaact ttgtatgtta ctacaagacc ttgtgttttg
    56701 tgtgcaaaaa tgtgcataaa tgcaggtata ttgaaaattg tttatgaagg tgattatcca
    56761 gatgatatgt ctacagaatt attaaaagaa gcaggaatag aactcataaa attttagtgg
    56821 ataaaattta taagacaata aatgtcaaat tatgacactg aaatacaatg gtatcaaatg
    56881 attaaaagaa agtatttgaa aaatcatcag attagtagta agatatattt tataattaaa
    56941 taaatttatg agatagatta attacatgtt atttatttta taataatgag ggatactatt
    57001 tatgagtaaa agaaatacac atacagaaat tgcaaagttg tttagtttaa taagtcaaat
    57061 aggattgatg attgtaatat caatattagg atgtactttc ttaggaaaat ttttagatag
    57121 taaattgaat actactccag ttctgacaat aatattttta ttgttgggtg ttggaggtgc
    57181 ttttagtggt gtttataaaa ctctaatagt atatacacga aggaagtgat atattggata
    57241 taaaattact taaccaggta aggtcagtaa caaaaggtat agtaatattt gatgtagttt
    57301 taattgtgct aatgttgata acatcaacat taagtaaacc agtattatta ggacttatat
    57361 ttggctctat aatagctatg ttaaatttta gattattggc gatatcactg gaaaaatcag
    57421 tgacatttcc tgctgggaag gcacaagctt atgcatcagc acaatatgct gttagattat
    57481 ttataatggc agttgttcta attgcatcag ctacagtacc acatttaaat ataatcggaa
    57541 cagcattggg attattgagc acaaagtttg tcatattatc aaaaaacttt ataaataaat
    57601 taaagaggaa ggaggcataa gtatgaatca agcgcattat tatctcatag gaaactttaa
    57661 gattagtgaa acaatactag taagttgggg aataatggcc tttttagcga tagcatctta
    57721 ttttttgact agaaacttaa aaaaagttcc aactagtaaa gttcaaatat tcttagaatt
    57781 tgcagtaggt ggtcttgcta aaatggttac agatgttatg ggtaaaaata cagtaaaaag
    57841 aatgccatat atgataccat acataggaag cttgttttta tttttcgcat gttcaaattt
    57901 aagcccatta cttggattaa ggtcaccaac aaccgactta gatacaactg ctgcatgggc
    57961 aatgattaca ttttttatga tttattttgc aggagttaaa tttaaggggt tatcatactt
    58021 taaagaattg gctcaaccac ttgccataat gttaccaatc aatattatag gtgagttagc
    58081 tagaccaata tcattaagtt tccgtccatt tgggaatatt ttaggtggaa caataataat
    58141 gggtctgttt tatcaacttt taggttttat ttctagttta ataccaggat tatcaatacc
    58201 aattggagaa atattaatac cagtcccatt acacttgtac tttgacttat ttgcaggatt
    58261 gttacaagca tatatattta taatgttaac aatggtattt atatcaagtg caggagagtt
    58321 agattaagaa atcaacttat attaagataa aaataaaata atttaaaaaa ttaaagtttt
    58381 aaggaggaaa taattatgga aacagcaata gtagcggcag catcagcaat aggagcaggg
    58441 atagcagttg caacaggtat aggtgcaggt ataggtcaag gtatagcagc agctaaagct
    58501 gcagaagcag taggaaacca accagaagct aaaggtgata taacatcaac tttattacta
    58561 ggagttgcta tagcagagtc ttcagcaata tatggtttgg ttatatcaat aatacttcta
    58621 ttcgtaaatc cattctttaa atacttagga atgtagattt agtccttaaa tattttgaga
    58681 taggtttata ggtaaatcat ctcaaactta taatcatgag atgaacttta tcatctcgtg
    58741 attataagta gttatgattt atggaaagga ggataattat ggaaaaagca ctagtaggta
    58801 taacgtggga atttgtgttc caaattgtta atacttttat aatattttta cttctgagaa
    58861 agcttttatt caagccagta ttaaatataa ttgaatcaag agaaaatgat attaaaagtg
    58921 atttggcaga aggcgaaaaa gcaaaaaatg aaggtttagc tctgaaaaaa gaatatgagt
    58981 caaaaatcaa ttttgccaaa gatgaaggtc aagaaatcat aaaacaagca actataagag
    59041 cagagcagaa atcagatgat atagtaaata ctgcaaagaa agatgctctt gatataaaag
    59101 aaaaagcaaa caaagatata gaacaagaaa gacaaaaggt tataaatgaa atcaaaaatg
    59161 atatatctaa catagcacta cttgcagcat ctaaagttat tgaaaaagat ttagataaat
    59221 ctaaacatga agaattgata gaaaacttta taaaagaggt aggggaagct aaatgataaa
    59281 tgtaatagca aatagatatg ccgaagccct atttcaatta ggagaagaag aaaattcaac
    59341 tgatgtctta ttcaaagaac tagaaaaagt agtagatatg atgactaaag ttagtaaaga
    59401 tttctacaaa gttttaaaat caccattagt ttcaaaatca gaaaagaaaa acttggtaga
    59461 aattattttt agtaaagaag taagttctaa cataaaaaac tttttaaaag ttttagtaga
    59521 taaagataga atatcatatt tagaagatat cgaattagct tataaagaat tattaaataa
    59581 gaaaaacaat gtaattgatg gagtagcaat aagtgctatt ccaatgagtg aaacagatat
    59641 taaagaactt gaagtcaaac tttcaaataa atacaacaaa aatgttacta tagaaaatgt
    59701 tgttgacaag actatactag ggggagttct agttagaatt ggaaatgaac aaatagatgg
    59761 cactgtgaaa actcgtcttg ataagatgaa agagaagcta tctgaagtaa tatcttaaga
    59821 atggcggtga acccatgaac ttaaaacctg aagaaataag ttctataatt aaacagcaaa
    59881 taaaaaatta tgagaataaa gttgagttga cagatacagg tagtgtttta acagttgggg
    59941 atggtatagc gagtgtatat ggattagaaa aagctatgtc tggtgagttg ctagagttcc
    60001 caggtgaaat atatggtatg gcacttaact tggaagaaga agtagttgga gctgttatcc
    60061 ttggagatga cagtgaaata aaagaaggcg atatagttaa gagaactgga agaatagttg
    60121 aagttccagt tggagaagct ttaataggta gagttgtaaa ctcactaggt cagccaatag
    60181 atggaaaagg acctatagcg tacacaaaaa ctagaccagt tgaatctgag gcacctggta
    60241 taatagatag aagatctgtt tatgaaccat tacaaacagg aataaaatca atagattcaa
    60301 tgataccaat tggtagaggt caaagagagt taataatagg tgacagacaa actggtaaaa
    60361 catctatagt tattgacact atactaaatc aaaaaggaaa agatgttatc tgtatatatg
    60421 tagcaattgg acaaaaacgt tctactatag ctcaattagt tagctcttta gaaaaaggtg
    60481 gagctttgga ttatacaata gtagtttcag caacagcatc tgaatcagca ccacttcaat
    60541 atatagcacc atatgctgga gcagctatgg gtgaagaatt tatgtacaat gggaaacatg
    60601 tacttatagt gtatgatgat ttaagtaaac aagctgttgc atatagagaa atgtcattgt
    60661 tacttagaag accaccagga cgtgaggcat atcctggaga tgtattctat ttacattcaa
    60721 gattacttga aagagcagct aagttatctg atgaattggg tggaggttca atgactgcct
    60781 tgcctataat agaaactcaa gctggtgacg tttctgcata tataccaaca aatgttatat
    60841 ctataacaga tggtcaaata tacttacaac cagagttatt ctattcaggt gtaagaccag
    60901 cagttgaccc tggtatatca gtatcaaggg ttggtggttc tgcgcaaatt aaagcaatga
    60961 aaaaagttgc aggaacatta aaacttgcat attcacaata tagagaactt gcagcattct
    61021 cacagtttgg ttctgactta gatgaagata ctaaaaagag acttgctcaa ggtgaaagaa
    61081 tcgttgagat attaaaacaa ggtgagcatc aaccaataaa agttgaaaat caagttatga
    61141 taatatatgc tgttataaat aatcatttag aggatattcc gatagataat atagctagat
    61201 ttgaatcaga attgtatgca tttgtagata ataattatcc ggaaatatca agaaaaatat
    61261 taggtggaga agacttcact catgatttaa cagatgcaat aaatgaattt aaagaaaagt
    61321 ttgttgttga agtataatcc tttttaaaaa aggaggtaaa ttaattggca ggagctggaa
    61381 tcaaagcaat caaaacacgt atgggaactg ttgaaagtac aaagcaaata actaaagcaa
    61441 tggaattagt tgcatcttct aagcttagaa aagcaaaaga aaaggctgaa gattcaagag
    61501 aatattttaa tactttatac aatacagtaa aagatattgc ccaaacaaca cgtggaatta
    61561 gaaatgaatt tttaaaagaa agagaagtaa aaaataaatg ctatgtagta gtagctggag
    61621 acagaggact tgcaggtgga tacaattcta atgtattaaa ggaatctgtc tctcatatga
    61681 aaggtaaaaa ggaaacagtg attactgttg gaaagaaagc acatgaattt ttttctaaaa
    61741 gaaactacaa tatagcaaaa gatataaata gtgttgaagc ttgtggatat gaagatgcga
    61801 cagaaatttc acaaactatt attgacctgt ataaaaaagg tgaagtagat gaagtttata
    61861 ttgcttatac aaaatttaaa tctgctttat cacaagaagt tgaaattttg aaattgcttc
    61921 cattagtctt tgaaaaagat acagacgaac aaggaatggc agtaaaagaa gaaaaacaaa
    61981 atagatcaaa agtacaatat ataccatcag cagaagcagt tttaagttat attgtacctc
    62041 agtttatatc aggtacagta tatggtggaa ttttggaatc ttttgcatca gagcaaggtg
    62101 ctagaagaac tgcaatggaa tcagccacag acaatgcgaa tgaaatgcta tctaatttag
    62161 agttaagtta taacagagca agacagtcag ctgtaactca agaaataaca gagatagttg
    62221 gtggagttga agctctaaaa tagggaggtt aggaaatggc aaatgtaggt aaagtagtac
    62281 agattgtagg agctgtacta gatgttaagt ttgatagtga acaaagccta cctaacttat
    62341 taaatgcgtt agtaataaag ttaggagata aagaaatagt tgcagaagtt gctcaacata
    62401 taggtgatga tactgtaaga tgtatagcta tgagtgcaac agacggactt gtaagaggta
    62461 tggaagttgt agatacagga gggccaataa gtgttcctgt tggagatgag actttaggta
    62521 gaatattcaa tgttcttgga aaaccagttg atgggaaacc agctccaaaa tctgcaccta
    62581 aattacctat acatagacca gcaccggcgt atgatgaact agaaactaca gcagagatac
    62641 tagaaactgg tattaaggta gttgacttac tagctccata tctaaaaggt ggaaaaatag
    62701 gtctatttgg aggagcagga gtaggaaaga cagttctaat acaagagctt ataaacaata
    62761 ttgctaagca acatggtggt atttctgtat tttcaggagt aggagaaaga acaagagaag
    62821 gtaacgacct ttatggcgaa atgagtgagt ctggagttat aaataaaaca gctctagtat
    62881 ttggtcaaat gaatgaacca cctggagcga gaatgagagt tgctttaact ggacttacaa
    62941 tggcagaaca ttttagagat gagcaaggac aagacgtttt acttttcgtt gataatatat
    63001 tccgtttcac acaagctggt tcagaagttt cagcacttct aggacgtatg ccatcagctg
    63061 ttggttatca gccaacatta gctactgaaa tgggtgcact tcaagagaga ataacatcaa
    63121 ctaagaaagg ttcaataaca tctgttcaag cagtatatgt accagcagat gacttaactg
    63181 acccagcacc agctacaaca ttctctcact tagatgcaaa aacagtttta tcaagacaaa
    63241 tatcatcact gggtatatat ccagcagttg accctcttga atctacatca aggattcttg
    63301 accctagtat agtaggaaaa gaacattatg aagtagctag aggtgtacaa tcaatacttc
    63361 aaagatataa agaacttcaa gatataattg ctatacttgg tatggatgaa ttatctgatg
    63421 aagataagtt aatagttgca cgtgctagaa agatacaaag attcttatct caatcattca
    63481 cagttgctga gcaatttaca ggaaatccag gtcaatatgt acctgttaaa gagactgtaa
    63541 gaggatttaa agaaattctt gaagggaaac atgatgactt accagagtca gcattcttat
    63601 ttgtaggaac aatagaagac gctgttagaa aagcgaaggg aagtatgtag tcatggccag
    63661 tgaattttca ttgaaagttg taactccaag taatactttt tatgatggag atgttgaaat
    63721 gatagtagtt agaactacaa aaggagacaa aggggtgttg aaaggtcata taccatttgt
    63781 ttcaggtata cctgtaggaa ctttaaagat aaaaaaagag ggaagtttta aagaggcgaa
    63841 aatagcagaa ggattcatta atgtaactca agataaaact gtcatattga cagaatctgc
    63901 tgagtggatt taataaatta atttagagta taaaaagtca ttttgaccaa aaaaactttt
    63961 gggtaaaatg gcttttttgc gttttggtgt taataaaata acgataaaat tgtaagtttt
    64021 tagtgataat aaattcacaa aatgtttaaa tttactgcca aattatgttg taattaataa
    64081 gataaagaag atttagatag cttttatgta agtgaaaaag tcttcatata agaaatacat
    64141 ataaaatttt tataaaattt aaaaaatttg cttagttgtg tagtataata aataatataa
    64201 tagatttgta tataatattc attacaaata attttaaaag ttacagtttt attataatga
    64261 ttattaaatt aagataagta acataaacta aataataatg tgtattatcg atagtaaatt
    64321 tataaattca ttattaatta ttgaaagaag tattataaat tttctggagg aacaatgaat
    64381 atatttgata ttggcgtaga tataattgaa attgatagaa taagaaaggc tgtagacaaa
    64441 aataataggt ttttggagaa gatatttaca gatagagaaa tagagtattt taattctaag
    64501 aattttaaag cagaaagtat agcaggtaat ttcgcagcca aggaagctat aagtaaatct
    64561 ataggtacag gaattagagt gtttaatttt aaagatatag aagttctcag aaatgaaatg
    64621 ggaaaaccaa tagtaaaaac ttataataat ctagcaaaaa tgtgcataga ttataatgtt
    64681 ttagaaataa aagtttcaat atcacattca aaagattatg ctatagcaaa tgcgataacc
    64741 ataattaagg actaaggagt gattacatta tgatggataa aacagcaaaa gaaataatga
    64801 caacagatgt aatagtagca aaacaagatg atagtatagc agatgtagct aatatgctaa
    64861 ttgcagaaaa aataggaggt ctgccagttg tagattcaga gaatagagtt gttggaataa
    64921 tttctgaaac agatattctt aaaaaagaaa aatatataga agcacctctc tatataaatc
    64981 tattacaagg attgattttc ttagatgact taaaaaaagt tgaaaaagat ataaaacaag
    65041 tagctgctta taaggttgga gaacttatgt ctaaagatat aataaaggtt catgaagatg
    65101 ataagtttga tgatgtagct aatgtaatga ttaaaaaatc aataaacaga gttcctgtag
    65161 tagatgatga taacaaactt aaaggtataa tttgtagata tgatataata aaagctttat
    65221 acaatgaata aactgaagta ggaggtgtga tgtgagaaaa aagtggacca tagtatgtat
    65281 tatgttcttg gcactggtta taattgtaat aggatgccaa aaacgacagt ccacaaaaga
    65341 agaagtatac aaagattttc aaaagcagat ttctgatatg aattattaca gttgtaaagc
    65401 tgaagtagaa gtagttggaa ataaaagtcc acacaattat gttttaattc atacttataa
    65461 aaaaaccgat aactataaat tagaagtaat ttccccaaaa catttaaaag ggaaaagtat
    65521 agaatatcaa ggtgataaaa tattagttaa aaatccaaag attagtgatg tagtggagtt
    65581 acctaataca ggaaaaaata atcaatatct ttttgtagga gactttataa aaaactatct
    65641 tcaaaatgag gagatgaaag taaagttatc caaaggacat ttggttttag agacttttat
    65701 tcctggggat aataaatatt ttaacaagca agtattatat gtaaatgctg acacaaaaaa
    65761 tcctgaaaaa atggaagtgt tagataaaga gggagtgcca agatttacag taaaatacaa
    65821 agattttgaa tacagaaact aagggggact aaagacatgc aaaaaataac agtgcctaca
    65881 tgggcagaga taaatctaga taacttaaga tttaacttaa ataatattaa aaatttatta
    65941 gaagaagata ttaagatttg tggagtaata aaagctgatg catatggaca tggtgcagta
    66001 gaagttgcaa aattgctaga aaaagaaaaa gtagattact tagcagtagc aagaactgct
    66061 gaaggaattg aacttagaca aaatggcata acacttccta ttttgaactt gggatatact
    66121 ccagacgaag cttttgaaga ttctataaaa aataaaataa ctatgacagt ttattcttta
    66181 gaaacagcac aaaagataaa tgaaattgca aaatctttag gagaaaaagc ctgtgttcat
    66241 gttaaaatag actcagggat gactagaata ggtttccaac ctaatgagga gtcagtacag
    66301 gaaataatag aattaaataa attagaatat atagatttag aaggtatgtt tactcatttt
    66361 gctacagctg atgaagtaag taaagagtac acttataaac aagctaataa ttataaattt
    66421 atgtctgata aattagatga ggctggtgta aaaatagcta taaaacatgt atcaaacagt
    66481 gcagctatta tggattgccc tgatttaaga ttaaatatgg taagagcagg aataatatta
    66541 tatggtcatt atccatctga tgatgtattt aaagatagat tagaattaag accagccatg
    66601 aaattaaaat caaaaatcgg acatataaaa caagttgaac caggtgtagg aataagttat
    66661 ggactaaaat acacaactac aggtaaagaa acaatagcta cagttccaat aggatacgca
    66721 gatggattta ctagaatcca aaaaaatcca aaggttctta ttaagggaga agtgtttgat
    66781 gtagttggta gaatatgtat ggatcaaata atggttagaa ttgacaaaga tatagacata
    66841 aaagttggag atgaggttat actatttgga gaaggcgaag ttacagctga gcgtatagct
    66901 aaagacttag gaactataaa ctatgaagtg ttatgtatga tatcaagaag agttgaccgt
    66961 gtttatatgg aaaataatga gcttgtacaa ataaacagtt atttgctaaa ataaaaccat
    67021 gatattataa tctgggaggc gattttgtgt acgataagag taaagaaaaa atagaagtta
    67081 ctttacctga ctctctgatt tctgaagttg atagtatagt tcagatggag aacagtaaca
    67141 gagaagattt tgcaaaagct gcttttcagt tctatataac tcagaaaaag aaaattcata
    67201 ttaaagaatc tatgaaaacg ggttatagag aaatgggtca aattaatctg tcactagctg
    67261 aattaggtat gacagttgaa gaagtatctt ctgatgataa aaatgaaggg aagattgccc
    67321 aaggtgagta ctaattgtga aataaagcgt ggtgatttat attacgctga tttaagtcca
    67381 gttgttggtt cagagcaggg aggcgttaga cctgttctga taatacaaaa tgatgtaggt
    67441 aataaatata gtcctacagt tatagtatca gctataacat ctcagattaa taaagccaaa
    67501 ttacctactc atgtagagat aagttccaat gaatatgggc ttaataaaga ctcagtaatt
    67561 cttttagagc aaattagaac tatcgataaa aagagactga gggaaaaaat tggttgtcta
    67621 gatgaaaata tgatgataaa agtagatgat ggacttcaaa taagtttagg attgtttact
    67681 ttttaaagag ggatttgtct tttattagac aatccctttt ttcttttaaa tatctcttaa
    67741 ggtttaattt taatgtacta tgttaataaa attaaaaata acgcaaaaaa atagcataaa
    67801 agtgatataa taatatggaa ttgatataaa attacgaact caagggaggt aattgcatga
    67861 gagaccataa aggattaaat gagattgcgc gtatcataag aagagatata gtttcaatga
    67921 tacatagagc caaatctgga catccaggag ggtcattatc agtagttgaa atacttactg
    67981 cgttatactt tgatgaaatg aatgtggatt cttcaaatcc taaaatggag gatagagata
    68041 gatttgtatt atcaaaagga catgctgcac cagcattata tgctacattg gctgaaaaag
    68101 gctattttga taaggaagaa ctgaatggat taaggaagat aggaaggatg ttacaaggtc
    68161 atccagacat gaaaggcacg ccaggtgtgg aaatatctac tggttcttta ggtcaaggtt
    68221 tttcagttgc atgtggtatg gctatggcat caaagttgga taatgctccg tggaatgtgt
    68281 atactttgct tggtgatggt gaggttcaag aaggaattgt ttgggaagct gctatgagtg
    68341 ctgcacatta taaacttgat aatttaattg cttttttaga taataatgga cttcaaatag
    68401 atggagatat agaaagcgta atgagtttag gttcaatagt tgataaattt aaagcttttg
    68461 gttggaatgt aattgagata gatggacatg attttgacca aatatttgcg gctcttgata
    68521 tagctaaatc aacagttaaa aaacctacaa tgatagttgc aaaaactata aaaggcaaag
    68581 gaatatcttt tatggaaaat caagcaggat ggcatgggac agctccaagt gatgaggagc
    68641 tagaaaaagc cttgcttgaa ttaggaggtg ctgacaatga gtaaaatggc gacgagagaa
    68701 gcatatggta aagcattagt aaaattaggt caaataaatg acaatgttgt agtattagat
    68761 gcagatttat caaagtctac aaaaacacat gatttttaca agtcatttcc agatagattt
    68821 tttaatatgg gtatagctga acaaaattta ataggcgctg cttgtggatt atcaactgct
    68881 ggtaaaatac cttttgcaag tacttttgct atgtttgcta cgggaagggc ttttgagatt
    68941 ataagaaatt cagtttgtta tccaaagcta aatgtaaaaa tatgtgctac tcatgcaggt
    69001 cttactgttg gagaggatgg agcatctcat gaaagtgtgg aagatattgc tataatgaga
    69061 gctataccaa atatgacagt tcttgttcca gctgatggag ttgaaacaga aaaaattatt
    69121 tttgaaatag ctaagtataa tggacctgtt tatgttagat tagggagaag ttcagtacca
    69181 gttttatttg atgaagatta taaatttgaa ataggaaaag gtacagtctt aagagaagga
    69241 aaggatgtat caattatagc ttgtggaatt atggtaaatg aagctctatt agcacaagaa
    69301 aagttgcaag aagaaggtat aagtgctaga gttataaaca tgtcctcgat aaaacctata
    69361 gacaaagatt taatattaga atcagcaaaa gaaacgaatg ctattgtaac tgtagaagaa
    69421 catagcataa ttgggggatt aggttctgct gtaagtgagg tagtaggaga gtcttgtcca
    69481 accattgtaa agaaagttgg aattaaagat acatttggag aatctggtac tccaaacgaa
    69541 ttgttaaaaa aatatgaatt aacttgtgat gacataataa agactgttaa agaagctatt
    69601 attgcaaaaa gaatgtaaga gatataaatt attcatgttt gttaataata taatatat
//
LOCUS       DACXMZ010000003        48836 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.003, whole genome shotgun sequence.
ACCESSION   DACXMZ010000003 DACXMZ010000000
VERSION     DACXMZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.003"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1180
                     /locus_tag="JD016_00645"
     CDS             <1..1180
                     /locus_tag="JD016_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAU4998132.1"
                     /translation="NCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHEKAGRDD
                     KYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFATSVVITP
                     EVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSSYLYGLP
                     HELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAAVDGGKC
                     VDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVYGMTGIS
                     SDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVGENGKAI
                     RADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARDTLRLVK
                     "
     gene            1273..1797
                     /locus_tag="JD016_00650"
     CDS             1273..1797
                     /locus_tag="JD016_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAU4998133.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1822..1998
                     /locus_tag="JD016_00655"
     CDS             1822..1998
                     /locus_tag="JD016_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAU4998134.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2205..2762
                     /gene="fapR"
                     /locus_tag="JD016_00660"
     CDS             2205..2762
                     /gene="fapR"
                     /locus_tag="JD016_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HAU4998135.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2773..3795
                     /gene="plsX"
                     /locus_tag="JD016_00665"
     CDS             2773..3795
                     /gene="plsX"
                     /locus_tag="JD016_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAU4998136.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3821..4807
                     /locus_tag="JD016_00670"
     CDS             3821..4807
                     /locus_tag="JD016_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAU4998137.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            4916..5845
                     /gene="fabK"
                     /locus_tag="JD016_00675"
     CDS             4916..5845
                     /gene="fabK"
                     /locus_tag="JD016_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HAU4998138.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5861..6811
                     /gene="fabD"
                     /locus_tag="JD016_00680"
     CDS             5861..6811
                     /gene="fabD"
                     /locus_tag="JD016_00680"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAU4998139.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6811..7560
                     /gene="fabG"
                     /locus_tag="JD016_00685"
     CDS             6811..7560
                     /gene="fabG"
                     /locus_tag="JD016_00685"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAU4998140.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7614..7838
                     /gene="acpP"
                     /locus_tag="JD016_00690"
     CDS             7614..7838
                     /gene="acpP"
                     /locus_tag="JD016_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAU4998141.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7867..9105
                     /gene="fabF"
                     /locus_tag="JD016_00695"
     CDS             7867..9105
                     /gene="fabF"
                     /locus_tag="JD016_00695"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAU4998142.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9331..11049
                     /locus_tag="JD016_00700"
     CDS             9331..11049
                     /locus_tag="JD016_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAU4998143.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     QYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGKY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDSIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11080..13080
                     /locus_tag="JD016_00705"
     CDS             11080..13080
                     /locus_tag="JD016_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAU4998144.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEEKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13428..13676
                     /locus_tag="JD016_00710"
     CDS             13428..13676
                     /locus_tag="JD016_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HAU4998145.1"
                     /translation="MMKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVE
                     GMNIEDVIQKLKRIECKTKPTSCPDQLSLALENYMNIK"
     gene            13711..14436
                     /locus_tag="JD016_00715"
     CDS             13711..14436
                     /locus_tag="JD016_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAU4998146.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14443..15771
                     /locus_tag="JD016_00720"
     CDS             14443..15771
                     /locus_tag="JD016_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAU4998147.1"
                     /translation="MGGLNMNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVA
                     SIFVYIFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCI
                     SWGALIVGFPTSLSAVWAPAGEPHIQKIIIVVMIVGLTIINLLGVSLSKIVQNVITVG
                     KLIPLILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAE
                     DMENPQKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMG
                     AGLVTFGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATML
                     ITIPIALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLI
                     AVCVGTWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYGENLKKIV"
     gene            15977..16516
                     /locus_tag="JD016_00725"
     CDS             15977..16516
                     /locus_tag="JD016_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU4998148.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16605..18590
                     /locus_tag="JD016_00730"
     CDS             16605..18590
                     /locus_tag="JD016_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HAU4998149.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18814..19782
                     /gene="spoIIIAA"
                     /locus_tag="JD016_00735"
     CDS             18814..19782
                     /gene="spoIIIAA"
                     /locus_tag="JD016_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HAU4998150.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19772..20293
                     /locus_tag="JD016_00740"
     CDS             19772..20293
                     /locus_tag="JD016_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HAU4998151.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20310..20507
                     /gene="spoIIIAC"
                     /locus_tag="JD016_00745"
     CDS             20310..20507
                     /gene="spoIIIAC"
                     /locus_tag="JD016_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HAU4998152.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20523..20909
                     /gene="spoIIIAD"
                     /locus_tag="JD016_00750"
     CDS             20523..20909
                     /gene="spoIIIAD"
                     /locus_tag="JD016_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HAU4998153.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            20988..22199
                     /gene="spoIIIAE"
                     /locus_tag="JD016_00755"
     CDS             20988..22199
                     /gene="spoIIIAE"
                     /locus_tag="JD016_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HAU4998154.1"
                     /translation="MLMKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGET
                     KNSIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGL
                     KILMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLI
                     GFKDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFF
                     KNFLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLY
                     VTSFDKFSVKTAKFAIGNFIPIVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICL
                     LPVIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVA
                     ILTSISVVSQG"
     gene            22259..22876
                     /locus_tag="JD016_00760"
     CDS             22259..22876
                     /locus_tag="JD016_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HAU4998155.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22888..23442
                     /locus_tag="JD016_00765"
     CDS             22888..23442
                     /locus_tag="JD016_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HAU4998156.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23469..24158
                     /locus_tag="JD016_00770"
     CDS             23469..24158
                     /locus_tag="JD016_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HAU4998157.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNIDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKEFQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24268..24630
                     /locus_tag="JD016_00775"
     CDS             24268..24630
                     /locus_tag="JD016_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAU4998158.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24712..25221
                     /gene="nusB"
                     /locus_tag="JD016_00780"
     CDS             24712..25221
                     /gene="nusB"
                     /locus_tag="JD016_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAU4998159.1"
                     /translation="MMKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDN
                     SEHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKH
                     AKNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGIL
                     GSVVDEIGK"
     gene            25205..26275
                     /locus_tag="JD016_00785"
     CDS             25205..26275
                     /locus_tag="JD016_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HAU4998160.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26272..27477
                     /locus_tag="JD016_00790"
     CDS             26272..27477
                     /locus_tag="JD016_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAU4998161.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27479..27694
                     /gene="xseB"
                     /locus_tag="JD016_00795"
     CDS             27479..27694
                     /gene="xseB"
                     /locus_tag="JD016_00795"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAU4998162.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27697..28584
                     /locus_tag="JD016_00800"
     CDS             27697..28584
                     /locus_tag="JD016_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAU4998163.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28614..29051
                     /locus_tag="JD016_00805"
     CDS             28614..29051
                     /locus_tag="JD016_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HAU4998164.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29085..30950
                     /gene="dxs"
                     /locus_tag="JD016_00810"
     CDS             29085..30950
                     /gene="dxs"
                     /locus_tag="JD016_00810"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAU4998165.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDEGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31083..31895
                     /locus_tag="JD016_00815"
     CDS             31083..31895
                     /locus_tag="JD016_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HAU4998166.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            31915..33594
                     /gene="recN"
                     /locus_tag="JD016_00820"
     CDS             31915..33594
                     /gene="recN"
                     /locus_tag="JD016_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAU4998167.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33809..34675
                     /locus_tag="JD016_00825"
     CDS             33809..34675
                     /locus_tag="JD016_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998168.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECIIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKYGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKFTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34768..35247)
                     /locus_tag="JD016_00830"
     CDS             complement(34768..35247)
                     /locus_tag="JD016_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU4998169.1"
                     /translation="MAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQDFNNE
                     LKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKIGKIL
                     LEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYMELKL"
     gene            complement(35320..35760)
                     /locus_tag="JD016_00835"
     CDS             complement(35320..35760)
                     /locus_tag="JD016_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU4998170.1"
                     /translation="MKLNVRIADINNWRDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            35921..36979
                     /locus_tag="JD016_00840"
     CDS             35921..36979
                     /locus_tag="JD016_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HAU4998171.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTIVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37121..37945
                     /gene="spo0A"
                     /locus_tag="JD016_00845"
     CDS             37121..37945
                     /gene="spo0A"
                     /locus_tag="JD016_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HAU4998172.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38358..39479
                     /locus_tag="JD016_00850"
     CDS             38358..39479
                     /locus_tag="JD016_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998173.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39497..40372
                     /locus_tag="JD016_00855"
     CDS             39497..40372
                     /locus_tag="JD016_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998174.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40396..41088
                     /locus_tag="JD016_00860"
     CDS             40396..41088
                     /locus_tag="JD016_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAU4998175.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41096..41920
                     /locus_tag="JD016_00865"
     CDS             41096..41920
                     /locus_tag="JD016_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAU4998176.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            41925..42992
                     /locus_tag="JD016_00870"
     CDS             41925..42992
                     /locus_tag="JD016_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HAU4998177.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            42994..43530
                     /locus_tag="JD016_00875"
     CDS             42994..43530
                     /locus_tag="JD016_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAU4998178.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43598..44203
                     /locus_tag="JD016_00880"
     CDS             43598..44203
                     /locus_tag="JD016_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998179.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIAVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44215..45129
                     /locus_tag="JD016_00885"
     CDS             44215..45129
                     /locus_tag="JD016_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAU4998180.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45171..46343
                     /locus_tag="JD016_00890"
     CDS             45171..46343
                     /locus_tag="JD016_00890"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAU4998181.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGEVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46390..47202
                     /locus_tag="JD016_00895"
     CDS             46390..47202
                     /locus_tag="JD016_00895"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAU4998182.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47391..48731
                     /locus_tag="JD016_00900"
     CDS             47391..48731
                     /locus_tag="JD016_00900"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAU4998183.1"
                     /translation="MEISIMRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQAS
                     ALLMSIYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIP
                     LVASCGAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSA
                     KVAIADKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEE
                     SFELAKSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVEL
                     CEVLGAYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLF
                     KKSKYKYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDY
                     VNEGEVLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
BASE COUNT        19744 a         4924 c         9181 g        14987 t
ORIGIN      
        1 aaactgtggg agttcttcat taaaatatca attaatagat atgaataatg aagaagtttt
       61 atgtatagga ttggttgaaa gaataggaat agaaggttct atattaaaac atgaaaaagc
      121 aggtagagat gataaatacg ttgttgaaca accaatgaag gaccacaaag atgcaatagc
      181 attagtttta gaagctgttg ctcatccaga atttggtgca gttaaagaaa tgaaagaaat
      241 agatgcagta ggacatagag ttgtacatgc tggagaaaaa tttgcaactt cagtagtaat
      301 aactccagaa gtagaagaag ctttaaaaga atgtatagat ttagctccac ttcataatcc
      361 agcaaacata atgggaatag atgcttgtaa agctatactt ccagatgtac caatggtagg
      421 tgtatttgat actgctttcc atcaaacaat gcctaaatct tcatacttat atggtttacc
      481 tcatgagcta tatactaaat atggggtaag aagatatgga ttccacggaa cttctcataa
      541 ttatgtatct caaagagcag cagagatatt aggaaaagat ataaaagatt taaaaatagt
      601 aacttgtcac ttaggaaatg gtgcttctat agctgctgtt gatggtggaa aatgtgtaga
      661 tacatctatg ggattcactc cattagaagg attaataatg ggaactagat gtggagatat
      721 agacccagct atattaccat tcttaatgag aaaagaaggt ttagatgctg atggattgga
      781 taaattaatg aataaagaat ctggagtata tggaatgact ggaatttcta gtgacttcag
      841 agatatagaa gatgcagcta aaaatggaga cgaaagagct caagctacat tagaagcgta
      901 tgttaaaaaa gtacaaaaat atataggcgc ttatgctgct gaaatgaatg gattggatgt
      961 tgtagtattt actgcaggcg ttggtgaaaa tggaaaggct ataagagcag atattgcttc
     1021 taacatggag tttttaggaa tgaaattaga taaagaagct aatgatgtta gaggaaaaga
     1081 aacagtaata tctactgctg attctaaagt aaaaatgctt ttaataccaa ctaatgaaga
     1141 attaatgata gctagagata cattaagatt ggtaaaataa ttaaattaaa cctatgccta
     1201 tcaagtaaaa atacttgaca aacaaatgct agatttgtat aataaatttg gtgatatgtt
     1261 gaggtgaact taatgaaagt tagcatagaa aaaataaata gaaaagaaac tgacaaaata
     1321 gacttgaatt tttgtgaaaa aattgatact attagttatt gtgatgagat ttacaaatta
     1381 gtatcacctg taaatttaaa agggaaagtg tcaaagacta ataaaggttt gtatctagat
     1441 atagatgtca attttacaat tgttgacaat tgttcaagat gtcttaaaga agtagaaata
     1501 ccactagagt attctataca aggtttttta gtaaaagaag aagattatga tgaagatgag
     1561 tttgaggaat ttgatccttt tatattcgat ggtgaagaaa tcgaccttat tgatataata
     1621 gaacagacat tagattttaa tgtacctcat aaagttcttt gtagtgaaaa ctgtaaaggt
     1681 ctttgtcaag tatgcggggc aaacttaaat gaagaagagt gttcttgcag tgaaattaca
     1741 aacgatgagg aatacataga tcctcgtttt gctaaattaa aagatttatt taattaaatt
     1801 ttgactaagg aggtggcgta aatggcagta ccaaagcgta aaacgtctaa atcaaacaca
     1861 aaaatgagaa gagcagctaa ctcaaagatg gaagcaacag gatttgtaag ttgcccacaa
     1921 tgtcatgagc caaaattacc acatagagtg tgtccagact gtggatatta taaaggtaaa
     1981 gaagttgtgt ctaagtaata gcttctaaaa agcatgtaga gtatactcta catgtttttt
     2041 ttgtacatat aaaaaaataa gatgatttac tagagaaaat aagtggattt tatgcatatt
     2101 aaaaaataga aaaataataa tataagtatt gaaaattttt gtgtgttact atatactact
     2161 attagtagct ggtactaatt acagctataa aatttggagg gaacatgaaa aagaaaagta
     2221 aagcccaaag acaaaaagag cttatagata tgctaaagac agatcctttt tatactgatg
     2281 aagaattatc aagtctgttt gatgtaagta tacagaccat aagattagat agaatgagtc
     2341 taaacatacc agagctaaga gaaagagtca aatcaatagc agaaactcag agttcaaaag
     2401 taaagacttt aggagtaaag gaaattacag gagaaatcat tgatttatct gtagggcgat
     2461 taggtatatc tatgttagaa gttacacagg acatgattta ctctaagact aatactttaa
     2521 aagatacgta tattttttct ctagcagatt cattggcaat ggctataata gatgcaccta
     2581 aggttattat gagagttgca aatgtaaaaa gttttaagtt gatagaacaa caagatagac
     2641 ttatagcaaa agcagaagtg tatagaaata tagataaaaa gcattatgta aaagttgtta
     2701 tcaacaataa agcccaagag caaatattta gaggaaagtt tatctttgaa gaattagatt
     2761 aggagggaaa ttatgaaaat agtaattgat ggaatgggtg gagataatgc acctaaatct
     2821 aatgtagaag gcgcagtaaa tgctataaaa gaatatcagg ttgatttaat aatcacagga
     2881 gataaagatt tacttgaaaa ggaattttca aattatgaat ttgatagaaa taaattagag
     2941 atagttcata ctactgagat tatagaaaac gaagataaac cagtaaaggc tataagaagt
     3001 aaaaaagatt catctatggt agtagcatta aatcttgtaa aagaaggaaa ggcagatgct
     3061 ataatatcag ctggaaatac aggagcctta cttgctggtg gattatttgt tgttggtaga
     3121 ataaaaggaa tcgatagacc ttgcttatgt tcagcaatac caaatgtaaa gcgaggaatg
     3181 acacttatag cagactgtgg cgcaaatgca gattgtaaac ctaagaatct agttgaattt
     3241 gcagctatga gtaatattta ttcaagaaaa gttttaggtc ttgaaaatcc taaggtagca
     3301 ttagcaaatg ttggactaga agaaggcaaa ggcaatgatt tagtcaaaag gtcatatgaa
     3361 gaaattaaaa aattagattt aaattttata gggaatgtag aagctagaga agttataaat
     3421 gcatacacag atataatcat atgtgatgga tttacaggta atatattact taaatctgct
     3481 gaaggtgtag cactttcagt tatgagtctt ataaaggaaa cttttatggc cagtacaaaa
     3541 agtaaaatag gagcattact tataaaggat gatttaagaa aattaaaaag ttttatagat
     3601 tattctgagt atgggggagc tccactttta ggtctaaatg gaggagttat aaaagctcat
     3661 ggtagttctg atgctaaagc tataaaaaat gctataaatc aaggaataaa attttcaaag
     3721 ggaaaagtcg ttgaagatat taaccaattt ataagcaaat acaatgaaga aaataaaaat
     3781 aacgaagatg aataaactcg ttttggaggt ttaatatata atgaatacaa aggctggaat
     3841 attaggagta ggaagttatc tacctgagca atcatatgat aattttcact tcgaaaaaat
     3901 aatggataca tcagatgagt ggataagtac tagaactggt attaaggaaa gaagatttgc
     3961 caaagagtct gaagcaactt ctgacttagc atctaaagct gccttaaaag ctatagaatg
     4021 tgcaaagtta aacgtagagg atatagagct gattatatta gctacaatta ctcctgatat
     4081 gtctttgcct tcaactgcat gtatagtaca agatgcaata ggagcagtga atgctactgc
     4141 atttgatata tcagcagctt gttctggatt tgtatatgga gtaactattg caaagcaatt
     4201 tgtggaaaca ggttgctata aaaatgtgct tgtaatagga gcagaaactt gttctaagtt
     4261 tttaaattat gatgatagaa caacagcagt attgtttgga gatggagctg gagcagctgt
     4321 tattggccct gtaaatgagg gtggaatact gtctacacat atgggctctg atggtaaagg
     4381 aaaagattgt ttaaaagtac ctgctggagg ttcaagactt aaggcaagta aagagactgt
     4441 agaagctaat ctacatacaa tagaaatggc aggtagtgat gtgtttaaat ttgcagttag
     4501 aaagatggca gaaacttctt taagagctct tgaaaaagca aatctaaata caactgatat
     4561 agactactta gtgccacatc aagctaatat aagaatcata caagcttctt ctaagagatt
     4621 agagcttgat atgaaaaaag tttatgtaaa tatagacaaa tatggaaata tgtctgctgc
     4681 atctatacca gtagcattag atgaagctta tagagaaggt aaaattaaaa agggtgataa
     4741 tgtagtttta gttggctttg gtggaggtct aacttggggg gcttcagttg tcaagtggac
     4801 tttatagtat aaaatatcta aaatgattta attaacaaaa ttaaacttat acacaaaata
     4861 aagttgatta ataataaaaa gtttactttt atgatttcaa gtgaaggaga attgtatgaa
     4921 taaaatttgc aaaatattaa atataaaata cccagttatc caagggggaa tggcatgggt
     4981 agctactgca tcattagcaa gtgctgtatc taatgcagga ggacttggca taatagcagc
     5041 aggaaacgca ccaaaagaag ctataaagaa agaaattgtt gagtgtaaaa aattaacaga
     5101 taaacctttt ggagtaaatg taatgcttat gtcgccattt gttgatgata taattgattt
     5161 gattatagaa gaaaaagttc aagttattac tactggtgct ggaaatcctg caaagtatat
     5221 ggatagatta aaggaagctg gaacaaaggt tattcctgta gtacctacaa tagctttggc
     5281 acaaagaatg gaaaagctag gagctacagc agtaatagca gaaggtactg aaggtggagg
     5341 acatatagga gaacttacta ctatggtctt agttccacaa gttgctgatg ctgtaaacat
     5401 acctgtaata gctgctggag gaattgtaga tggtagagga attgcagcat catttgcatt
     5461 aggtgccagt gcagttcaag taggaactag atttatttgc agtgaagagt gttctgtcca
     5521 ttcaaactat aaaaacttag tactaaaagc aaaagataga gatgcaattg taacaggaag
     5581 aagtactggt catccagtaa gaacattaaa aaataaacta tcaaaagaat ttttaaagat
     5641 ggaacaaaat ggagctactc ctgaagaact ggataaaaaa ggtacaggag ctttaagatt
     5701 tgcaacagta gatggagaca tagaaaaagg ttcatttatg gcaggtcaaa gtgctgctat
     5761 ggtaaaagaa ataacacctt gtaaggaaat tatagaggct atggtaaatc aagcaagaga
     5821 gattatgcca gcaatagaac tgtaaatcag gaggagcaag atgggaaaag ttgcactagt
     5881 attccctggt caaggagctc aatatgtagg tatggccaag gatttatatg aaaataatac
     5941 tgttgctaaa agtgtaatag atgaggctag tgatgcctta aatatggatt taaaaaacct
     6001 tatgtttaat ggaaatgaag aagaactttc taaaactgaa aacactcaac ctgctatggt
     6061 tacacatagt gtagcagtat taaaagcggt acaagctcaa atagatttaa aatataatgc
     6121 atgcttagga ttatccttag gagagtatag tgcgttggtt gcagcagatg caattgactt
     6181 taaagatgct gtatgtttag ttaaaaaaag aggtaagttt atgcaggaaa cagttccagc
     6241 aggtgttgga gctatggcag caattttagg tttagataga aatgtacttg aaaatgtggt
     6301 taaagatata caaggaggta ttgtagaagt agcaaattac aattctcctg gacaaatagt
     6361 tatatctggt gaaaatgaaa agatagaaga agcaatggta aaatgcaaag aagcaggagc
     6421 aaaaagggca gttaaattaa atgtaagtgg accttttcac tcatcaatgt taaaagaagc
     6481 tggtgtaaaa ttggccagtg aattagaaaa agttaacata actaaaccta aagttgatgt
     6541 ggtggcaaat gtaaatgcag attactataa aaacgaaggt aaagacctgc ttataaaaca
     6601 agtaagttca tctgttcttt gggaagattc tatagaaaga cttttaaatg atggatatga
     6661 tacttttata gaaatgggac ctggaaaaac tttaaaagca tttattaaga agatagcttc
     6721 caataaaaaa gcaaatgtca atatttataa tatagatggt atagattctc ttaatgaatt
     6781 tgtacaaaat tatagaaatg gagagatata atgataaatc ttacaggaca agtagcagtt
     6841 gttactggtg gctctagggg tataggaaaa gaaatagcaa aaaaactagc atcttttgga
     6901 gctgatgtag taatcaatta tacttctaaa gaagatgaag cactaaaaac taaaaatgaa
     6961 atagaaagta tgggggtaaa gtgtacctct ataaaatgtg atgtatctaa atttgatgaa
     7021 gtgaatcaaa tgatagattc tgttgtaagc gaatttggaa aaattgatat attggttaat
     7081 aatgcaggca taactaaaga tggtctgctt atgagaatga aagaagaaga ttttgataga
     7141 gttatagata taaacttaaa aggtgtcttt aattgtacaa aagcagttac taaacctatg
     7201 atgaaaaaga agtatggaag aataataaat atgacttcag tagttggaat tatgggtaat
     7261 gcagggcaaa ctaattattg tgcatcaaaa gcaggtgtaa ttggatttac aaaagcttct
     7321 gcaagagagt tagcatcaag aaacataaat ataaatgcag tagcacctgg atttatagaa
     7381 acagatatga ctaaagtact aagtgatgat gtaaaagaat caacactagc aaacatacca
     7441 aagaaatctt atggtaaacc agaagatgta gccaatgccg tagcattttt agttagtgac
     7501 atgtcaagtt atataacagg acaagtaata aatgtagatg gtggaatggt aatgcaataa
     7561 taatttaaaa agtagagaaa tctattaaaa taaaaaattt taggaggtac acaatgttta
     7621 ataaagttgt agaaataata atagagcaat taggagtaga agataaagaa ataactatgg
     7681 agacatcatt aatgaaggat ttagaagcag attcattaga tgcagtagaa ataataatgg
     7741 cacttgaaga tgaatttggt atagaaatac cagatacaga agcagaaaac ttcaaatcta
     7801 taggagatat agttaagtat atagaagcta ataaatagtc gagctttaaa aaagtggagg
     7861 tatccgatga ataaaagagt agtaataaca ggacttggat gtgttacgcc gttgggaaca
     7921 ggaaaagaag agttctggag taatataaaa tctggagtat ctggtataga taaaataact
     7981 aattttgatg ctagcacata tcaaactcag atagcaggag aagttaagaa ttttcatcca
     8041 gaagaatata taagtaaaaa ggaattgaag agattagata aatttgctca atttgcaata
     8101 gtatctgcta aattagcagt tgaagatgca aatcttgatt tagataaagt tgatagagaa
     8161 cgttttggtg ttataatagg ttctggaata ggtggagttg aagctataga aactcaacat
     8221 aaaattcttt tggaaaaagg aaataaaaga gtaagttcac tttttgtacc tatgatgata
     8281 ggaaatatgg cagctggtca agtttcaata tttttaggag caaaagggcc taacactaat
     8341 gtatgtacag cttgtgcatc aggaactcat tcaataggtg atgcttttaa ggtaattcaa
     8401 agaggtgatg cagacattat ggtagcaggg ggttctgaag ctgctgtaac aggtcttgct
     8461 tttgctggat tctgtaacat gaaggctatg tcaactagaa atgatgaccc taaaactgca
     8521 tcaagaccat ttgataaaga cagagatggt tttgtaatgg gagaaggagc aggtattgtt
     8581 atcttagaag acttagaaca tgctttagca agaggtgcta aaatatatgc agaagtagtt
     8641 ggatatggtt taactgctga tgcatatcat atgacaactc cagctgaaaa tggagaggga
     8701 gcagctaggt ctatgaatat ggctttgaaa gatggaaatg ttccattaga agaagtagat
     8761 tatataaatg ctcatggaac ttcaacttat tacaatgatt tatatgaaac tatggctata
     8821 aaaactgtat ttggagaaaa ggcatatgac ttatgtgtgt catcaacaaa atctatgact
     8881 ggtcacttat taggagcatc aggtgctata gaggctgttg tttgtgctat gagtatagaa
     8941 gatagttttg tgcctccaac tataaacata caagaagttg gtgaagattt ggatttagat
     9001 tatgttccaa atcaaggtaa agaaaaaaat attagatatg cactatcaaa ttctttagga
     9061 tttggtgggc ataatgctac aatagtacta aaaaaatatg tataaataaa tattaaatat
     9121 aaaactaaaa aacaagcact tgttttaact gtataaattt atattagctt aaatttaaag
     9181 tctcctaaat tattttagga gactttttta ttaagattta agtctaggat atgttgtaaa
     9241 tcattttgat atattgtatt attaattatt aagaggttat ttatatatgc aagttttcat
     9301 tctacaatat acaaaaggag gactaaaatt ttgaatttta gactaccaaa gaaaaggtta
     9361 cccaaaaaga taatttttat atgtataata cttattagta ttattttgtt tataaagtat
     9421 gaccaaaatg gattgaaaga aagagaaatt ataaataagg gacaatatat gtctgaaagc
     9481 cttgtggcag ataaggagta tgatgaagca aaaaaagtgg ttaagacttc tttcgaatct
     9541 attccagaaa aaaaatacaa taaagtatat aatgaagttt gggatatgtt aaagactatt
     9601 tcatatgttc cagatggaac gaagatagtc attgattctt tagaaaagat gagagaatca
     9661 gatgtgaaat tatctgatga atgtagattt aatatagaaa aaagattagc atcagtgtat
     9721 ataatggata ataattattc taaggcagta gatttaactg taaagtctat aaagttggct
     9781 gagaaattgg ggaataatta tgaaaaagca agactagatg ttgatttggc gagcatattt
     9841 ttaaagatag gtggctatga aacaggagag aaattaataa aggattcttt taaaatagat
     9901 attgatgaag gtgaaaaaaa tggaatggtt agattatatg ctattataaa tctagcagaa
     9961 atatatgtag aagagggaaa atatgataaa gctattgaaa taagtagcag gataaaagat
    10021 tataaaaagt ttgtaaatac ggataattac aatgattttg atataatggc ctctataatg
    10081 cagtcaaatg cgtatgtggg caaaaataat atacaaaagg caaagacttt tttagaaaaa
    10141 gctgattatc ttataaaatc agatagaaca gtttatatat tagataaaga tatgtattat
    10201 tatatggcaa tgggaaactt agaatataaa agcgaaaatt ataattctgc aatagataat
    10261 tataaaaaaa gtttagagat atctaccaaa agaaagttaa ctcaggagaa aataaaaaat
    10321 ttaaataaga tattaaatat atatgaaaaa caaggaaata tagaatcttt aaatgagtat
    10381 caaaagatgt taataaatga atataaagag aacaaaagta taagagattc agagactagt
    10441 ttctatataa ttgataaagt atcaaacgaa agtaagctat ttgaaaaaac taaaaaagaa
    10501 ataaaatatt ataaaatatt atttacagta attttaattg ctatattagg gtctactttt
    10561 ttatacaacc gattgaagta ttttaaagca caaaatttac atgatggtct tacaaatgtt
    10621 tataatagaa aaagttttga tattatgtat gaaaagtaca gagagaaaga tgataatttt
    10681 gcacttgtga tgatagatat tgataatttc aagctcataa atgataatta tggacataaa
    10741 tttggtgaca ctgttataaa gggaataaca aataccattt gtgttatgtt agaacaagat
    10801 gataaagtct ttaggtacgg aggagaagaa ttttctgtat taataagaaa taaatctggt
    10861 gaggaagtag cagatataat tgacagtata agggttgcaa ttgcaaataa aaaatggaat
    10921 gatgatgtga ctgtgactat aagtgcaggt gtagctcata tttcagatag caaagatgta
    10981 ctagaagaag ctgataaaaa tttatataaa gcaaaacaac ttggtagaaa taaagtagta
    11041 tataagtaat tatatactat ataaataaga ggtataaata tgaaacaatc aatagctata
    11101 gtaactgata aaataactaa actatcatct ttcttagaag aaaatatacg tttggtttta
    11161 ggtaattata ttgatataaa ccactattat ctggaagttt taaaagagaa tgataaaata
    11221 caaggtgatt ttgtactagt aatgaatgat gatagattaa acagtatgag aaagcattta
    11281 gtacaagata gtaagattat agtgataagg agaacactta aagaaaatga aatatatgga
    11341 ttattttcaa taccaaatgg aactgaagta ttggttgtaa atgatacaaa agaaactacc
    11401 ttagaaacga taagcttatt ttacaaaata ggagtaaaaa atttaaagtt tactccatat
    11461 attgaaggaa aaatttataa aaatatagac atagctataa cgcctggagt aaagggaaaa
    11521 gtaccaagtt atataaataa agtaatcgac cttggaaata gatatattga tatatctacc
    11581 tttatagaaa taataaatgg tcttagaata gatgttaaag aaatacgtaa aaatttaatg
    11641 gcatattctg agagtttaat aagtttagat aatggagtta aagataatta taggcaactg
    11701 tttttaaaaa tagaagaaca agatgttata ttgaatctat caaaagatgg aataatattt
    11761 acttcagcag atggtataat aaacacattt aataaagaag ctttaaaaat tttaggtata
    11821 aatgaaaatc cacaaggcaa aaacatagaa gatatcattt ctgaagattt aaatatttta
    11881 cttgaagaga aagaaataat tgatgaagtg gtatttacaa ataaaaaata tataaatgta
    11941 aataaaaaaa gtattttttc tatggggaat aaagctggga tatattataa cttacaagaa
    12001 ataacgtata taaaaaaatt agaacaaaat cttactaata aattaagaga acaaggtcag
    12061 atagcaagat atacatttaa agatataaaa actaaaaata aaaatatggt aaagtgtgta
    12121 gaattggcta aaaaaatatc taaatcagac ctttctgtat taataatagg agaaagtggt
    12181 actggtaaag aattattatc acaatcaatt cataatgcct ccaatagaag taatcaacca
    12241 tttatagcag ttaattgtgc agcagttcca gatagtctat tagaaagtca actgtttgga
    12301 tatgaaaaag gctcttttac aggagcttta agagaaggaa aaaagggtct atttgaactt
    12361 gctaataatg gaactatatt tctggatgaa attggagata tgcctccatt gctacagaca
    12421 aagttactaa gggtattaca agaaaagcag gttatgccta taggttccca taatgttata
    12481 aacatagatg ttagggtcat agcagcaaca aacaaaaact tactaaagat gattagagaa
    12541 ggaaaattta gagaggacct gtattataga ctaaatgtat tgccaataga tgtaccatca
    12601 ttgaggaata gaaaagaaga tataataaca cttatgaatt tctttttgaa aaacaatata
    12661 agactttcgc cagaagttat aactatttta gaaaaatata attggccagg aaatataaga
    12721 gagttacaaa atgttgcttc ttatgtaagt ctaatgtgtg atagtatggt ttttagagat
    12781 gacttaccac catatataag ggtagaatgt gctaacaatt atgaagatgg atttggttat
    12841 acaagaaaag aatttgagga aatactgagg attctatatg aaagaagaga gtcatcctct
    12901 ggaattggaa gagggtttat aatcgaaaag cttatacagg aaggtattga gatttctgaa
    12961 agcaaagtta gaaaggtgct atcttattta agtgaatgtg gatacatatc atctaaatct
    13021 ggtagaggag gaagtcaaat aacctcagag gggataaaac tttataataa tataaattaa
    13081 gctaagtatt tgatattgaa atcacatatt ttgaatttag atatttaaat aataataaac
    13141 ttttatattt atagagatta atttatatta tgtcagaaag aatattgtaa tttataatta
    13201 aatattaata ggtttttaaa ataggattag ataggtaata ggttaagtta gaataaccca
    13261 ttacctattt ttatttgtaa gaaaagtgta catagaatta gaagtaaaaa atacaactaa
    13321 taaaactatt gaaaatacta gataaataat aaataaaaat aattatagat tttggcatac
    13381 atattgctaa ttatagagat aagacgacaa aataattggg aggtttaatg atgaaataca
    13441 aaacaacagg gacttgtgct acagaaattg aatttgaagt aaaagaaaat aaagttacaa
    13501 atgtaaactt tataggtgga tgtgatggaa atttaaaagg gctaaaagta ttagttgaag
    13561 gcatgaatat agaagatgta atacaaaagt taaaaagaat agagtgtaaa acaaagccaa
    13621 catcttgccc agaccaactg tctctagcat tggaaaatta tatgaatata aagtaataca
    13681 taaaacaatc gattagttag gagtttaatt atgaaaccaa taatagggat tttaggaaat
    13741 ttaataataa tggaaaatgg tatgtttcca ggtcttgaaa ggtcatatgt aaataatgat
    13801 tatataaatg cagtgctaaa aggtggcggt agtccagtaa taattcctgt aaatacagat
    13861 aaagaagtta taaaaaaaca gattgaaatg gttgatggag tcttaatatc tgggggatgg
    13921 gatatcaatc ctcaactgta tggagaagaa acaagagaag aaacgacatt tatatatcca
    13981 gaagtggatg aatttgattt aattgcaata agtatagcat tagaacttaa aaagcctata
    14041 ttaggtgtat gtaggggact acaaatttta aatgtaagtt taggaggaac tttatatcaa
    14101 gataataatc ttatagaagg aagttatata aaacatacac aatcttctaa aagacatgta
    14161 gctactcata aagttgatgt taaagaagga agtattttag agggaatttt aggaaaacaa
    14221 ttacttacaa atagttatca tcatcaaagt gtcaatcagc ttggaaaagg attaaaagcc
    14281 atagcatact caaaagatgg tataatagaa gctattgaga aagaggatga aaattttgta
    14341 gtaggagtac agtggcatcc agaaatgatg gtagattact gtgataaaat ggaaaaacta
    14401 tttaaatatt ttataaatat ttgttcaaat ttataatata ccttgggggg attgaacatg
    14461 aacgaaaaga acaaaatggg attaataagt attatacttt taggtattaa tgcagtagta
    14521 ggagctggag tttttctatt accaggagat gcaatgaaat catttggtgt agcaagtata
    14581 tttgtatata tatttgatat gttgttagtt ttatctatgg cattctgttt tgctgaggtt
    14641 gcaggtaaat tcaataaaaa tggtgctgcc tatgtatata caaaagaagc ttttggagat
    14701 ttttgtggtt ttgaagtagg tcttatgaaa tgggtaatag gatgtatttc ttggggagct
    14761 ttaatagtag gttttcctac atcattatca gcagtatggg ctccagcagg tgaaccacat
    14821 atacaaaaaa ttataattgt agtcatgata gttgggctta caattataaa cttattaggt
    14881 gtatctctat caaaaattgt acaaaatgtt ataactgtag gtaaactaat tccattaata
    14941 ctgtttatag gaataggtat attttttata aaaggagtta attttacttc ttcaacaatg
    15001 gtacctccag gagctggagc aacagagttt ggagcagcag cacttttaat gttttattca
    15061 tttacaggat ttgaatctat agcagttgca gcagaagata tggagaatcc acaaaaaaat
    15121 ataccaatag caattataag tgtaatagta attgcatcaa taatatatat attaaatcaa
    15181 gttgtatgtg ttggtatact aggagattcg ttatctagta catctacacc agtggcagat
    15241 gctgcaagaa tatgctttgg aaatatggga gctggattgg ttacctttgg aaccttggtt
    15301 tctgttggtg gaatttgtat gtgtggtgct tttgtaaatc caagaagttg tgtagcttta
    15361 gctgatgata aaatgttacc gagaatattt gcaagaaaag ataaaaaagg tacaccatat
    15421 gtagcgataa tagctactat gttaatcact ataccaatag ctctatctgg aagttttgct
    15481 gaattagcag caataagtgc agttgctaga ttcatacaat acataccaac atctttatcc
    15541 gtattagtgt ttagaaagaa aagaccagaa ttagtaggta cttttaaaac tccttttgga
    15601 gcagtgattc ctctaattgc tgtatgtgtt ggaacatggt tattattcca agcatctatg
    15661 catcaattaa ttatgggatt aggagctttg gcaattggag taccattata ctttataatg
    15721 aagtcttata atagaaaagt ctatggtgaa aatttaaaga aaatagttta attatgttat
    15781 aacagtttat tgagttaaag ttaggtattt gcagaaaatt aaatacaaat aagtatctat
    15841 aaggctatat taaaatatta ttaatttttt attttaatat agccttaaat tgttatcaat
    15901 aagtgaaaaa tattgtaaca atagaagaac aatagtatta tgtttataga caaaaaactt
    15961 attaggaggt aaatttatgc caacaataac attaaaaaat ggtgtagacg tacttattag
    16021 agaaggagta agggaagatg ctcaaagcat tatagatttt tataatgaag tgggtggaga
    16081 aactcatttt ctgtcatttg gaaaggatga atataaaata tctttggagg aacaagaaaa
    16141 tgctatagaa tcagcaaaag catccgataa ttctgttaaa ctgattgcat ttatagatgg
    16201 tgaaatagtt gggattgcaa cgattgattc taatcaaaaa gcaaaaggaa agcatgttgg
    16261 agtattagga attgttgtaa aagaaaaata ttggggaatt ggattgggta aaaggcttat
    16321 gttagacctt atagaatggt gtaaatcaaa tggaataacc aagaagataa ctcttgtcac
    16381 taatgaagaa aattataatg ctataggatt atacaaaaaa gttggatttg aagtagaaag
    16441 catactaaag aaagaatgtt attataatgg tgtttatact gatttaatag gaatgtcact
    16501 tttattggga atataattag tgtacatttt atctcgaaaa aagtatatta agtatggtat
    16561 aattaaaaaa gaatataact taatataatt ttggaggacg aattatgaaa aatctgtgta
    16621 aaatatcaga tgattattta aacagcaagc ttaagtattt aaagttatta tcaaaacaat
    16681 atccaagtat atcaaaagca agtacagaaa taataaattt agaggctata ttaaatcttc
    16741 caaagggaac agagcatttt ataacagatg tacatggtga atatgaacca tttgtacatg
    16801 tattaaaaaa tggttctggt gtaataaaga gaaaaataga agaattgttt tcaaatacaa
    16861 taagagacag cgaaaaaaaa atgttagcaa ctcttgtata ttatcctgaa caaaagttag
    16921 acctaataat taagcaagag gaaaatatag atgattttta tagaataaat atttatagac
    16981 ttatagaact ttgtaaatat gcttctagca aatatactag gtctaaagtt agaaaactgt
    17041 taccagaaaa ttttaaatac ataatagaag aacttttgca tgagcatgtt aaaagtgaac
    17101 ataaagaaga gtactataaa agtatagttg aaacaatagt tgacatagga atagctaagg
    17161 agtttataat tgctatttct acagttatac aaaaattggt tgtagataga cttcatgtaa
    17221 taggagatat ttatgataga ggtccaagac cagatataat tgtcgataag cttattgaac
    17281 atcattgtgt agatattcaa tggggaaatc atgatatatt gtggatgggt gcagcctcag
    17341 gtgaaaaaac ttgtatagca aatgcattga ggatatcagc tagatatgcg aatttagaca
    17401 ttgtagaaga catatatgga ataaatctat tgcctttagc cacttttgct atagaaatgt
    17461 ataaagatga cccttgtaaa gaatttattc caaaggttaa tgaccaaagt gtaacaacaa
    17521 cagaaaagtc tttaatggca aaaatgcaca aagcaataag tataattcaa tttaagcttg
    17581 aaggtgaggt tataagacga agacctgaat ttgaaatgga acatagactc ttgcttaata
    17641 tgataaacta tgatgaaggt acgattaatt taaaaggtaa aacatataaa ttaaaagata
    17701 cttatttacc tacaatagac aaaaaagacc catataaatt aactatggaa gaaagaaatg
    17761 ttattgataa attagtatct tcatttagag gtagtgaaaa attacaaaaa catgtttcat
    17821 ttttgttttc taaaggaagt atatatttaa aggctaactc aaatttactg attcatggat
    17881 gtgtaccgtt aaatgaagat ggaagcttta tgtctatgaa tataatggga aaagagtata
    17941 aaggtaaggc tcttatggat aagatggagt ctctagcaag agaaggattt ttctttaaag
    18001 ataaggcaga agaaaaactg tatggcatgg atattatgtg gtatttatgg actgggaaat
    18061 gttcatcatt atttggtaaa gatgatatga ctacatttga aagatacttt atagctgaaa
    18121 aagagactca taaagaaaat aagaacccat attttaaact tagagaaaat gagatggctt
    18181 gtaaaagatt atttgaagaa tttgatttag aacttgatga atctcatata ataaatggtc
    18241 atgttcctgt ggagagtaaa aatggagaga gcccaataaa agctaatggt aaaattctcg
    18301 ttatagatgg tggtttttct agggcttacc aaaaaactac tggtatagct ggatatactt
    18361 tgatatacaa ttctagaact ttgcaattag tatcgcatga accatttaat tctgctgaag
    18421 aagccattgc aaatgaaagt gatatcttat cgacaactgt tgtagtagag cataaggcaa
    18481 agagaaaaat ggtgagagat actgatgaag gagtaaagat acaagaggaa atagaagatt
    18541 taaaactact tttaatggct tataagaaag ggctaataaa agaaatgtag atgttataga
    18601 ggcacttcat gattgaagtg tctttttgtt aatacttatt aaatatatta taaagattaa
    18661 taatagtagt ttatattata cttactaagt gaatccaaga taaatgggtt cacttttttt
    18721 atttataaac attattaaaa aaatatagat gttttggtta tattccttgt cctttataaa
    18781 tagaattata taaacaaagg aggaggacaa gagatgaata aactttctga tgaaataata
    18841 aactctcttt ctacaactat aagagaaaag gtagaaaaag tctcaaacaa taatctgaac
    18901 atagaagaaa ttaggttacg ctcacaaaaa ccattgatac taaatgcaaa ctctaaagat
    18961 tatttttaca atcaaaaaac tatgacactt gatttaaatc aacaaaactc atatgtagta
    19021 acaagagaag atgtagagca aacttttcaa ataatatgta aatattcaat acattcattt
    19081 atggatgaca taaaaaaagg attcataact ttgagaggag gtcatagagt agggttagtt
    19141 ggaaaagcaa tagtagaaga tggacaagtc aaaaatataa agcatatatc atctttaaat
    19201 ataagggtat caagggaaat tattggatgc tcggataaga ttttaagtca tatcataaag
    19261 ggaaaaaatc agattaataa tactttgata atatcaccac ctcaatgtgg aaaaacaact
    19321 ttaataagag atatagtaag aaatttaagt aatggtaatg aagattatgg atttaaaggt
    19381 ttaaaagtgg ctttagtaga tgagcgtaat gagatagcag gagcttatct aggtgttcca
    19441 caaatggatg tgggaataag gactgatata atagaaactt gcccaaaaga cttgggtatt
    19501 acaatgcttt taaggtctat gtcaccaaat gttattgtga cagatgaaat tggaagtgaa
    19561 aaagaaataa aggcattata cacagcatta aatggaggta taggattaat aactactgtg
    19621 catggagatt caatagagga tatacaaaat agaaaagaat taaatagact tctggataaa
    19681 gagttattta aaaaggttat aattctttct gcaaaaagag gagcaggaac aattgaaaaa
    19741 atttatgatt tagaagaaaa gagatggtat tttgcaaatt aaaataatta ttatagcttt
    19801 cttgatagga agtagttatt tgataggtga acaaatatac aaaacttata ctcgtagaca
    19861 taaacaatta aatgatttaa taagagtttt ggaaattttg aggatggatt tatcttttgg
    19921 actttataca ttagaagaaa tctttaatag gataggtgga aataaagagt tttgtttttg
    19981 gaaatttttc tatcaaatat cagaaggact tcacaatgaa caaagtaaaa ctttggaaat
    20041 aataatatca gaaaatattg atgtcttaag taaagaaact tatctaggca ataaagaaat
    20101 tgaggaactg aaaaatctaa tacttacttt agggaaaagc gatatagagt cacaacaaag
    20161 aatgatagat ttatctatag aaaatttgaa aaaacaaacc tatgagacaa aggaagatat
    20221 aaacaaaaaa ggcgtattgt ataaaaaatt agtgacattc ataggtatag gtatatgtat
    20281 tatattgatt taatatgggg aggtttatta tggaaatatc attgatatta aaggttgcag
    20341 gtgtcggtat tctaatatca gtattgaata tgatattgga aaaaacagat agaaaagatt
    20401 gggcaggtct tactacatta gcaggtgtaa taatcgtatt gggtatggta ataacagaaa
    20461 taagtgactt gtttaatact gtaagaacaa tgtttcaact ttactaaaga ggagggatta
    20521 atttggaaat aatgcaatta ataggaattg caatcatttc aactacactc tgtttagtaa
    20581 ttaaaaaaga tagaccagag atagcaaatt tcatagctat aataactgga gtaataatac
    20641 tattgtcagt tatgtttaag ctaaatttta tagtagatag tatacaagat ttggctaaca
    20701 aggctaacat cccgactatg tatatatcat taataataaa attaattggg atagcatact
    20761 taatggaatt tgcgatacaa ctttgtaaag attgtgggga aggaaacatt gcttcaaaac
    20821 tagaatttgg aggtaaaata atagttatgt caatgtcttt ccctatactt ttgtccattg
    20881 tagaaatggt ggtgaatata attccataga tgtgtatctt ggaatgaata taagtatatt
    20941 agagagcttt cgttgtattg aggtttatag agtgaagaga ggcatggttg cttatgaaaa
    21001 aaagattttt accaatgatt ataggttttt tatttacttt cttttttgta aatatctttg
    21061 ctattatgat atttgctaat gaagtaccaa gtagtgaaga taaagaatat ggtgaaacaa
    21121 aaaatagcat agataagtat atagatggtc aattagacaa gctagatata aatgagattc
    21181 aagactatat aaataaagaa atagttatta atgatgttaa tttaaaatca tttgtaaaag
    21241 atttaattag tggagaaaaa aatatcttag atttattcaa taaagatgga ttgaagatat
    21301 tgatgtttga tgaatttaaa gcaagtttaa aggtagtagc agtaattttg gtactggctc
    21361 tgttatcatc tattttaaag agtctggaaa actctttttc atcaggtgca gtaagtcaaa
    21421 tagcaacata tataatattt atcacaatgg tatcacttac acttattggt tttaaagatg
    21481 tattacagat ttgctatgat gcaatagacc atactgtagg tcttatgcaa gttataatgc
    21541 caatattaat aacattttta cttttaattg gattcccaat aacgtcgact actttgaatc
    21601 ctatattcat aggaggagta acatttataa atgtattttt taaaaatttt ctatttgtat
    21661 ctattactgt tgcatttgga attttaataa taaacaattt atcaaaaaat ataaggctaa
    21721 agagattttt ctcctttgta aaacaaataa attatgtttc tattggagct atgtttactg
    21781 tttacttagg tcttgtatcc atacaaggat tgtatgtaac cagttttgat aaattcagtg
    21841 taaagactgc taaatttgct ataggtaatt ttattcctat tgtaggtgga tttgtatctg
    21901 actctgtgga tattttatta tcttcatctc agcttataaa aaatatattt ggaggaatag
    21961 ggcttatctt acttgttgga atttgcttac ttccagtaat aaaaatttta tcagtaatag
    22021 tagtgtacaa attagcagca ataattgtag agcctgttgg agaggatgga atatctaatt
    22081 ttttaaatga agtggcaaat ttaatgataa taatgctagc atcagtaatt gctataacag
    22141 taatgttctt tgtaactgta gctatcttga cctcgataag tgtcgtaagt caagggtaat
    22201 ttatatatca gtgaaaaata attacattta atctaatgtt tttagagagg aggatgctat
    22261 gttagagggc ataaaggcat ggatagttag tgttttgata ggagcattta tagtaaatat
    22321 agtagatatg attttaccaa gttctaagat aaaaccttat gtcaacctag ttttaaactt
    22381 tatgtttgta tttattgtaa taactccagt agtaggattt ttttcaaaag atatgagttt
    22441 agaagataga atattaaaat ctatgggaaa ctacaacaag cagtatgtag atagtacaaa
    22501 tgctttggca aaagagactg gaaataatag tctatccaag ggatatgagg atgggttaaa
    22561 agaagtactt aaattaaaac ttgatgaata tggttatgat ttagaagata tagagcttaa
    22621 tggggcaaat ataaataata taaaaataaa agaaaagaat aacagtgcaa aaagtaataa
    22681 cagttccaat gaagaaaata aaaataatag tactaaaaga gaggaagaaa acataaactc
    22741 taatgacaaa gaaaattcta aacaagtctt caaaaaaggt acagagtatg ggttgaacct
    22801 aaatgaagaa aaattaaaaa acgacttaat aaaggtgctg gatgtttcta tagaagatat
    22861 acaaattgat aaataggagt tgggagaatg ttgaaaaact taaatgacaa agataagaga
    22921 aaggtatatt cactaataac tatagcagga atatgtgtgg tttcattagt attattatct
    22981 tgttttccta gtagcaaaag cgataaacaa gttggaaaaa cagagactaa taaaaatgct
    23041 gaaaagcaag tcactaaaga acaagaaaaa gatgatttag agtcaaaatt aacagcaata
    23101 ctatctaaaa ttgatggtgc aggagacgtt gatgtaatgg ttacatttga atcaagtgaa
    23161 gaaatacaac ctgcttttaa ttcaaataat acaacagaaa caacagagga aaaagatgca
    23221 caaggtggag aaagaacagt tacgacttct agtgaaaata aaacaatgat aacttcaaat
    23281 tcaagtgacc ctgtagttat aaaaactaca gagccaaaga taaagggagt gatagtggta
    23341 gcaagtggag caagtgaccc aagtgttaaa gagacacttt atagtgcagt acaaacatca
    23401 ttacaagtag ctggtcatca agtagaaata tattctaaat aaatacatat aaccgggggg
    23461 ataaatcaat gaagtttaat tataagggaa gaggatttgt aataataact ttaactgcaa
    23521 tgttagtggt agtaggaaca gtgaattatc aattaagtaa gaaatcttta ttagaaacat
    23581 caaaagagtt taaggcatat gaagaagcac agttgcaaaa aaatactgat gagagtgata
    23641 gtagtaagac agaggattct agtaatatag ataaacaaga tggtaaggag agtgctgata
    23701 ttgatatagt cgatagcaaa gctagtaaag taaaagaaaa ggcaactgag acaagtaagg
    23761 aaataaaagc tcaattatca tcagagaaaa atatgaaaaa ggcatcttat attttagata
    23821 tgaaaatgaa tagagaaaaa caaagaaatg agttagttca agatttaaat gaaatgataa
    23881 ataatccatc tacaacagaa gaatctagaa aagaagcttc taatatgaaa ttaaatatag
    23941 ttaaaattca agaaaaagaa tttcaaatag aaaacctttt gagtacaaaa ggatatgaag
    24001 aagcgttagt ttatataagc gataacaaag ttaatgtagt tgtaaatgaa gctaaattag
    24061 aaaagaaaga tgcagcaaaa atatttgatt tagttgcaga acaagccaat gtgaagtatg
    24121 aaaatattaa acttacaaat aataatagta ataagtaaag tataaacctc agaattaact
    24181 gaggttattt tcttgcaaag tagacttgta tttggtatta ttatacttat aaaaatagtt
    24241 ccttaaaatc aggaggtaag ttttaatatg gaagataata aatttggaca agtaaaaata
    24301 tctaatgatg taatagctac aatagctggt ttagcagcat tagaagttga aggcatagaa
    24361 actacagcaa cattaacaga taagttgtta aaaaataatg gagtaaaaat acaaatcgaa
    24421 gaagaggatg ttaatttaga tgtaatggtt acgataaagt atggaatgtc aataccagat
    24481 acagctttta aagttcaaga aaatgtaaaa aatactgttg agacaatgac aggactgaag
    24541 gtttctcaag taaatataca cattcaagga atcagcttta agaaggataa agttgataaa
    24601 gaagaagcaa aagcaactaa gaaaaattaa tattagccct ctgaaaagtc agagggtttt
    24661 ttgctggtaa ttgattttat atggataatt aaacttaaag gagaattttg attgatgaaa
    24721 aaagataggg cacaaaagag tacaacaaga gaatatataa tgaaacttat ataccaaata
    24781 aatattaata aagaagattt tgaaacttta gaagataaag tagataattt tttaaaagat
    24841 aattcagaac atattataaa tagatataaa gaacttgcac ttcaatactc aaaaaataca
    24901 aatttaaagt tagaagatac tgaaattgaa gatgttatag acaaaaaata tataaataca
    24961 gtatgcaaag cattgaaaga aaatcatgac aaaatagatg agttaataaa taagcatgct
    25021 aaaaactgga cagtagatag aatgccaaag gtagatgttt ctatacttag attgtctgtg
    25081 tgtgaaatac tttatttaga cactcctaat aaagtatcaa taaatgaagc agtagaactt
    25141 gcaaaaatat attgtgatga taagtctcct aaatttataa atggtatatt gggaagtgtt
    25201 gttgatgaaa ttgggaaata atataataat tggaattgat actagctgct atactacttc
    25261 tatagcagct atctctttag ataagaaagt tatattcaat gaaaaaataa tgttagaagt
    25321 aagggataat tcaaaaggtt taaggcaaag tgaagctgta tttcaacata tcaataattt
    25381 agggattctt agtgatagga taaaatcttt taaagataag tttaatgtag aaggagtttg
    25441 ctcatcaaaa aaaccgagac ctgttgaaaa ctcatatatg cctgtattca atgtaggtca
    25501 taattttggg aaattattgt ctagtatata tggttgtagg ttttatgaga ctactcatca
    25561 agagaatcat attgaagcaa gtttattaaa tagtaaatta aaaaataata ataaatttat
    25621 ttctgttcat atgtctggtg gtactacaga gatactttta acaagcaaac aagatagtca
    25681 tcacaatgta tgtgatacta atttaggtaa aattgccaaa ataagtatta aaaaagatga
    25741 taaaagtaag ttatacaata attttggata taatatagat ataattggag gaagtaaaga
    25801 tattagcttt ggtcaactta tagatagagt aggaattaag cttggatata aatttccttc
    25861 aggaaagtat ttggatgaaa atgctttaaa ttgtaatctt aaaatagaaa gtggtctgaa
    25921 aacttctgta agagatgggt atatgaattt atcaggatta gaaaatcagg taaataagat
    25981 tataaatgat aatggagata atactaatca aaaagaatat atatctaaat tggtattaga
    26041 ttcagtagtt agaaatatgt ttaaatcttt agtgtattta tgtgaaactt ataatgtaaa
    26101 tgaagtagta tttgctggtg gagtttctgc aagtaagtat attcttagag aattaagcat
    26161 gaagttaaga aaaaagcata tagaagctta ttttacagaa ccacaatatt caactgataa
    26221 tgctgtcggg tgtgctataa taggattgaa taacttttta ggagaaagag tatgaaacta
    26281 agagctttag atataagtga agcaaactca tatattaaaa gaattttaat aaacgaccca
    26341 atactttcta atcttaaagt taaaggagaa atttctaatt ttaaggttca tagtagtggg
    26401 aatgtatatt tatcacttaa agatgaaaca tcaaagttaa actgtgtaat ttttaaaagt
    26461 aattttaatc gcaatttaaa attagataat ggtgtaaaaa ttatagctaa tggatatata
    26521 tcagtctatg aaagagatgg tgcgtatcaa ttatacatta atgaaattga aatagaggga
    26581 ataggaaatt tacacataga gtttaataga ttaaaggaaa aacttaataa agaaggatta
    26641 tttgacccta aatacaagat acctatacct aaaatgccaa actcaattgg cgttataaca
    26701 tctccaacag gtgctgttat aagagatata ataaatgtaa taaaaagaag atatccgaaa
    26761 gttaatataa agctttatcc agtaacggtt caaggagata aatcagctga agaaatctgt
    26821 gaagcaatta gatttttcaa tcatatgaag aatgttgata cacttatagt tggtagaggt
    26881 gggggttcga tagaagaact ttggtctttt aatgatgaaa tggtagcaag agaggttttt
    26941 aattctcaaa ttccgataat atcagcagtt gggcatgaaa cggattttac tatttgtgac
    27001 tttgtgtctg atatgagagc cccaacgcca tcagcagcag ctgagattgc tactccatca
    27061 ttggatgata taaactataa acttggaaac ataaaaagta ggatgagtaa gtctcttaca
    27121 aatcaaatag agctagacca atataggtta gaaacagtat ttaataagat aaataactat
    27181 ttagattcgt acacaataaa agataaagtt atacaattag acaaaatata tgataaaata
    27241 atttttggaa tagaaaataa tttaaaatta gaagatgaaa aattagtaaa aattggagca
    27301 ttactccata atttaagtcc attagctact atggatagag gttatagtat aactcaaaaa
    27361 aatgggaaag taataaatag tataaaagga ttaaaaataa aggatagtat agatatagta
    27421 ttaaaagatg gaaatttaga atgtatgata gataaaattg aaaataaaga gggatagtat
    27481 gaatttaacc tacgaagaag cttataaaag attagaaagt attttaagtg aattagaatc
    27541 taaaaatgct agcttggatg aatctttaag cctatatgaa gaaggtataa gcctttataa
    27601 acattgtaat aagcttttag atgatgctaa attgaaaata agtaaattta atcaattggg
    27661 aatagaagaa gactttaaaa tagaggagga ataaaattgg aatttaaaca atgtctaaaa
    27721 gaaaaagcaa gttttgtaga aaaagtactt aaagaatata tgcctaaaga agaagggtat
    27781 caaaaaactg ttattgaagc tatgaactat agcttaagtg ctggaggtaa aagattaaga
    27841 ccaatactta cattggaggc ttgtaaaata gttggaggta atgaagatga agctattcct
    27901 tttgctattg caattgaaat gatacataca tattcactaa tacatgatga tttgccagct
    27961 cttgacaatg atgatttaag aagaggaaga ccaacaaatc ataaagtata tggagaagct
    28021 atggggattt tagcaggaga tgctttgtta aactatgctt ttgaagtaat gcttgcaggc
    28081 tcaatcaata aagaaaatcc agaaaagtat ctaaaagcta taaatgaaat tgccaaaggt
    28141 gctggtatat atggaatgat aggtggtcaa gtagtagatg tagaaagcga aaataaacaa
    28201 attgaaaaag aaaaattaga ttatatacat atgaataaaa ctgctgcaat gatggtaggc
    28261 tgtatgagag caggagctac tataggtgga gcaaattctg aacaaatgga agaaataact
    28321 aaatatgcaa aaaatatcgg attatcattc cagatagtag atgatatact agacatagta
    28381 ggagatgaag ctaagttagg taaaaaggtt ggaagtgata tagaaaatca taagtcaaca
    28441 tacccatctc ttttaggttt agataaatct aaagaaattg cacataattt gattgatgaa
    28501 gcaaagaaaa gtatagaaaa attatctgat gatgtagatt ttttaaaggg attggctgaa
    28561 tatataatag atagagaata ctaaaatata cagctaaaat cgaggaggga aaaatggatt
    28621 ttttttcgga gatttttaac aatggggctt tgggaataag tctgattgct tgtttcttag
    28681 cacaatttat aaaaatattt actggtaagg aaaaaagaat tgagttatca agaatactta
    28741 tttcaggagg tatgcctagt tcccatagtt cttttgttac aagtttagct acagtggtag
    28801 gtattgaaaa agggtttaat tctactgatt ttgctataat taccgtgctt gccttaatta
    28861 taatgtatga tgcagcaggt gttagacggg ctgttggtaa acaagctact atattaaatc
    28921 aaatggtggc agatatacag catggaaagc atatagagca gaagaaatta aaagaattaa
    28981 taggtcatac accactagaa gtttggtttg gagctttact tggaatagta acagctttaa
    29041 tattgatgta aattgaaaat ttacagaata aaggtgagga taatatgtat aaatatttag
    29101 ataaagtaaa ttctccaaag gatataaaaa atatgagtat agaagaaatg gatttacttg
    29161 caaaggatat aagaaagttc ttagtaaaat ctgtttcaaa gacaggagga cacttagctt
    29221 ctaatctagg agtggttgag ttaacattag cacttcataa agtatttgat agtcctaaag
    29281 ataagattgt atgggatgta gggcatcaat cttatgttca taaaatagtt actggaagaa
    29341 aagattgttt tgtatctttg agacagttta atggactaag tggttttcca aaggagaatg
    29401 aaagtcctca tgatattttt gatacaggac atagcagtac atctatttct atagctacag
    29461 gtattgcctg tgctagggat ataaaaaaag aaaactatag tgttatatct gtgataggag
    29521 atggttctat aacaggaggt atggcacttg aagctttaaa tcaattagga tatatagata
    29581 ccaatatgat agtaatcctt aatgataatg agatgtccat agataaaaat gttggaggaa
    29641 tgtctaaata tctgtcaagt attataagaa actcaacagt tgagaaaatg acagatgaag
    29701 ttgataaaat tttaaatgtt acacaaacag gtgaaatttt atctaagact gcacatagat
    29761 ttaaagataa actaatgtat agcttttctc ctcaggactg ttcatttttt gattcattag
    29821 gtataagata ttatggacct atagatggcc ataatactaa agaactaata gatatattga
    29881 gaaaagctaa acacaaaaaa gggcctgttc ttttacatgt aattacgaaa aaaggaaaag
    29941 ggtacagatt tgcagaagaa caacctgata aatatcatgg agtatcaaag tttgatataa
    30001 agacaggtgt tacatctgca aaagttaagt ctatgtcaat tagtgttgga gaaaaacttg
    30061 ttgatatggc taatagtaat gaaaatatag tagcaataac agctgcaatg ccatctggta
    30121 cagggttaaa cctatttgaa agtgcatatc caaaaagata ttatgatgtt ggtatagctg
    30181 aacaacatgc aacaggattt gcagcaggtc ttgcaaaaaa tggtatgaaa ccttactttg
    30241 ctgtgtactc atcatttttg caaagggcat atgaccaagt tattcatgat gtatgcataa
    30301 caaaaaagcc agttactttt cttatagata gagctggatt agttggaaat gatggagaaa
    30361 ctcatcatgg tatgtttgat ttaagttact taaattctat tccaaatatt gtagtaatgg
    30421 ctccaaaaga cacaagagag atggagctta tgatggattt gtcattaaaa ttagattgtc
    30481 cactagcaat tagataccca agaggaagta gttattacct agataaggga gagtatggag
    30541 agattgtact gggtaaatat gaggtattag atgagggtca agatacagtc atactttgta
    30601 ttggaagtat ggtaaaacat gctttagagg ctaaagaaat attatcaaga gaaggaataa
    30661 atccgacaat agttaatgct agattcttaa agccaattga tgaaggtatg ttaaaagcat
    30721 tattaaaaaa tcataaaaat gtagttacta tagaggataa tattgtaact ggaggatttg
    30781 gaagtagaat aaataagttt attatagata atgaatataa cgtaaatata cttaatatag
    30841 caataccaga agaatttgta aaacatggca atatagatga attatatgac tttgtaggat
    30901 tatcgccaaa aagtattgct gataagataa ggaaattagt tattgagtaa aaatctaatg
    30961 tagatattgt aaattaaaat ttatataaaa tatagaattt atcaattaga ataaatatca
    31021 agtgtgttac ttcaagctac tttagttatc attaaagtag tttttaacag aaaggtaatt
    31081 gtatgaaaaa aagaatagat ttattattag tagaacaagg gtactttgaa agtagagaaa
    31141 gagccaaaaa agcaataatg gctggattgg tttttgttga taatcaaagg tgtgataagg
    31201 ctggtacaga agtaaaagaa gattgtagta tagaagtaaa aggaaatcca ataccatatg
    31261 tcagtagagg tggtttaaaa cttgaaaaag caatgaaaaa ctttgatttg actattgatg
    31321 gaaaggtatg tatggatata ggggcatcca caggtggttt tacagattgt atgctcaaaa
    31381 atggagcaat aaaagtattt tctatagatg ttggttatgg tcaacttgca tggaaattga
    31441 gacaagatga tagggttgtg tgtatggaaa gaaccaatat aagaaatgtg acaatagaag
    31501 atacaaagca gtttgctgat tttgcgtcaa ttgatgtgtc ttttatatcg ttaaagttag
    31561 tacttccaaa ggcaaaggag ttagtacgtc atgatgggga agttgtcgct ttaattaaac
    31621 ctcaatttga agcaggaaga gaaaaagtag gtaaaaaagg agtagttaga gaaaaatcta
    31681 cacatataga agttataaaa atgatttctg atttctcagt cgaaaatgga tttgagattt
    31741 tagggttaga tttttctcct attaaaggtc ctgaaggtaa tatagaatat ctaattcatt
    31801 tgagaaatgg aaatgaagga tatgaatttg atggtgagac atacaataac aaaatagtag
    31861 aagttgttga agcttctcat aatttagaca aataggttaa aagggggctt agatttgatc
    31921 ctcgaattat atatgaaaaa ttgtgcctta gttgaagaac taaggttgaa tattgataaa
    31981 aacctaaata tacttacagg tgaaactggt tcaggaaaat ctataataat agatgcactt
    32041 ggactttgtt taggtgataa atatgatagg tcttttttaa gaaaaggaac agataaggga
    32101 cttgttgagg ctgtattttt ttcagacaat agatatctta aaaaaatatt agaagaaaat
    32161 gatataagta tggaagatga taatttattg gtaataacta ggttgatata ctcagatggt
    32221 aagagtactg ctagagtaaa tggaaggact gttaaagtgt ctcttcttaa agaaattgca
    32281 tctacactta tagatataca tggtcaacat caaaatcaag ctttgtttaa taaagatact
    32341 catttaaaat ttctagacct ttttggagaa aatgaattgg aagagtttaa aattgcttat
    32401 aaaaatgtgt accataagta tagtgaagta aaaaaagctt taaattgctt gacagaaaat
    32461 aaagatgaga tgcaaattca acgagaaata gatttattaa gatttcaaat aaatgaaatt
    32521 gaagctgcaa atctaaataa gaatgaatat gaagatttat taaaacaaag agaagtttat
    32581 agaaatagtg aaaagatata taataatcta aattcgagtt atagcaagtt acataatggt
    32641 gagtataatg ttattgatct aataggtctt gcatctaagg aacttaatga catatctaaa
    32701 tatgacagtg ttttaagtga atatagtgat actgtagaaa gaatcatgta tgagttacaa
    32761 gacatatctg gtgagataag aaattacaaa gataatatag actttgaacc ttatgagcta
    32821 gagcagattg agcttagaat cgacgagatt aataacttaa gaagaaaata tggagacagt
    32881 atagatgata tatttgagta ttatggaaaa attaaagata gattagatga aatattaaac
    32941 agagatgaaa gagttgaaca gttaagaagc aaactgatga atatagaaga agatttaaaa
    33001 ataaaagcca gtaaattgac taaagcaaga atagaagtag caactacttt agaaaaaata
    33061 cttcttgatg aattaaaaag cttaaatatg aaaaatgtaa tgttcaaggt taattttgaa
    33121 aaaggtccat ttactttaga tggagtagat gatattgaat ttatgatatc ttttaactta
    33181 ggagaagata ttaagcctat atataaagtt gcatcaggtg gtgagatgtc cagatttatg
    33241 ttagcgttta aaaccatatt ggctgatata gatgatattg atacattagt gtttgacgag
    33301 atagatactg gaataagtgg gatagcagca cagatagttg gtgagaaatt gagtgatata
    33361 gcaaagaaaa aacagataat atgtataact catttacctc aaattgctgc aaatgcagac
    33421 actcattatt gtatagagaa agatacttca aataatagga cgtttaccaa tgttagtaag
    33481 ttaaatgaat ctcaacgaaa aaatgaaatt gccagactta tagcaggtaa taatataaca
    33541 gaaaaaacta ttgaacatgc aagtgaaatc attgaattgg ctaaaaaatg ttagtgaaaa
    33601 acaagcattt taatagttgt aaagaatttt tatagaaatg ctttacacct attttttata
    33661 atgaaattat tttaaaatat ctagtgccta tattgaagaa aagtaggttt gaaaatggaa
    33721 taaaatttgt ataaatttgt aactttgaaa tttctaaatg cttaagtata atattattta
    33781 ttaaggtagt agtgagggga ggcataaaat gaataaaaaa gatactatgc acttaatttt
    33841 tataattgta atttttattt tattatggta tattgtggta gcagtaagtg agattaataa
    33901 aaatattgaa agttgtagta acaaattaga ttatttgtca caagagctta tgagtactaa
    33961 gtcggatatt agtaatacta taaatgaaga aatgtcaaaa agttatataa caaaaagtat
    34021 tgacttaaaa gtaaaaaaaa tcgagaaaaa agaatgtatt attgatgtag atgttcaatt
    34081 aagtaaactt ggaaaaaatg gaaaagtatt ttttatgtat aaagaagata gtgataaatg
    34141 gaatgaaact gagatgacaa aatatggaga attatcatat gcatgtgaaa taaagataaa
    34201 tacaggaagt gaatatgact ataaagtagt tacaagtgga gctatatctg aaagttctga
    34261 tgtgcaaaaa tttactaaat ctgattatat gcctgaacaa cccatattta attctgggat
    34321 aagagataat agagaatact ttattgaaat agtagaaaat agtaatcttt tcaatcatga
    34381 gagtgaaaag tcaaaaaata aagcttacga taatattaga ttagaaaaag tagatattat
    34441 tgtaaatgaa ggaaaaaaag acactatata caaagccaaa ttagctgaag gcttagatga
    34501 tggcaaaact aataattcaa atagaaatca agtgaattat gaagtaagtt ttcctaaaag
    34561 ggatattgat gacataattt atattaaagc taaacttaca tacaataatg gagtagaata
    34621 tacaaaggat ataactgaag atataactat gggattacaa gatttagaaa aataaataca
    34681 tatttaaata aatattcaaa tagatttgtt caataaaata gagtataaat aaaactgtag
    34741 tagttttact tatactctat taaaaaatta taattttaat tccatatatc tagccccttc
    34801 aattggatta taagtataag gttctatatc ataaaaacca aacttttcat ataatccttg
    34861 tgcacttttc atgcttggaa gagtatctaa tctcatgtgt gtatatccaa attttttagc
    34921 ttcttcaatg atttcttcca atagtatttt tcctattttt aatcctctaa attgatttct
    34981 tacatataat ctttttaatt cacatatttt accttcaagt ttttttaaag caacacaacc
    35041 tgccaaattt tcatcaacaa atgccaatat caatgagcct gaaggttttt tatattttcc
    35101 tggaagtgtt tttaattcat tattaaagtc ttgaaaacat aagtctatat ttagagaatt
    35161 tgaatattca gtaaatagaa gttttacgtt ttcaaggttt tctaaatctt tttcttcaac
    35221 aaattgtaaa gttatatttt gagccatttt atccgccatt atatacacat gtttatataa
    35281 tgacttcacc tccttgatat atgtatattt ttcttaatat caattagatt ttaataccat
    35341 tactttttca ccaccatagt caagctctcc attaaactca aatccaaaat ctttataaag
    35401 cttaatagcc attttattgt cttcgaatat actaagatat agttcatcac agttatattc
    35461 atttttaaga tgatttacaa gaaaatctaa tgatgctttt ccatatcctt taccttgata
    35521 ttttgaatca atcataaatc tatcaagcca aactctatca tcttctaaac gaccatacat
    35581 agcaaatcct attaaagaat tttcatcata tattccaaca ggtacccaag ctgttataaa
    35641 tttagattca gcaatagaaa gtgcattgct ttctatataa tctaactgac ttttatctac
    35701 agacaaacta actaaatcac gccaattatt tatgtctgca attctcacat ttaatttcaa
    35761 gatattaccg cccttcaaac tttttgttac aatctaatac tatcatatta tgtattaaaa
    35821 atcacttatt aagattttaa ataaactttt taaatgaata aattctcagg aaaaaggaat
    35881 tataaataca taataataca gcataggagt agatggatat atgaacaaaa taaataaaaa
    35941 tttcaaaaat atttggataa tattaggagc tgtatcgatt tttaccatag ttataatttt
    36001 taataaaaat tgtaatgtta ataaagagac tcaaatagaa gcttttacaa ttaagaatca
    36061 tgataaaaaa tttgtatatc ctatgggaaa tataattgga gtaaaagcaa atacagatgg
    36121 tgtattggtc ttaggatatg aggaagagga tgtagactat attggaggca tacaaatcgg
    36181 tgataatata gtaaaaatag ataataaaag gataaaaaat agtcaagatg tatcagagat
    36241 tttgaataag attaaaaaaa gtaaagttga agtgactttt gagagaaaaa ataagtataa
    36301 aacagaagta atagagacta aaaaagaaaa tggcaagtat aaacttggat tatgggtaag
    36361 agataaaata tctggaattg gaactatgac tttttacgac cctagtatgg aaaagttcaa
    36421 agcaatcggt catgccataa aagattcgga tactaatgaa ctattaaaga taaagcaagg
    36481 atatatatat aagccagaac aattaaaaat agtaaaagct agtaatgaaa aagtaggtaa
    36541 aataaaagga gattttaatg acagcaattt aatgggtaat ttctcaaata attcagagtt
    36601 aggtataagt ggtaatatta cagaaaacca taataaagag tttaatgtag caaataaaga
    36661 aaagcaatta atagaagttg gaagaccgca agatgtaaaa ataggagatg cagtaattct
    36721 ttttgaggat aaaaacaaaa atataacaag ctatgatata aaaatagaaa gtatagtata
    36781 tgataaagga aattatagag atatggtaat aaaagtagta gatgacaagt tattggaata
    36841 cacaggaggt atcgtacagg ggatgagtgg agctccaata atacaaaata ataaaattat
    36901 tggtgcaata actcatgttt ttagagataa tccgaaaaaa ggatatggta tttttataga
    36961 tgaaatgata aaattgtagg tgaggcatta aaaaatttta ttattttatc aattatctag
    37021 gaggaatata attttggagt gtcgaatatg ctttagagta gataattagg aagcaattgt
    37081 gtaaaaagtt tagttttctg taataagaag atgtttttta atggggggat ttttagtgga
    37141 aaaaatcaaa atagttttag cagatgacaa taaggatttt tgtcaggtat taaaagagta
    37201 tttgtctaat gaagatgata tcgatatatt aggcatagct aaggatggaa ttgaagcatt
    37261 agacttagta aaaaagacac aaccggactt attaatacta gatgtaataa tgccacatct
    37321 agatggatta ggtgtaattg aaaaattgaa tactatggat atacctaaaa tgccaaaaat
    37381 aatagtacta tcagcagtag gtcaagataa gataactcaa agcgcaataa atctaggagc
    37441 agattactac atagtaaagc catttgattt tgttgtgttt ataaacagaa ttagagagct
    37501 agtttctaat agagttacac aagttgagcc aaaacctaga ccagttcaag agactcaaat
    37561 gactagaagt gattttgtta agaatgtagg aaatatagag acagaaatca caaatataat
    37621 acatgaaata ggagtaccag ctcatataaa aggatattta tatttaagag aagcaataaa
    37681 aatggttatt gataatgttg agcttttagg tgcagtaaca aaagagttat atccaagtat
    37741 agctaaaaaa ttcaatacaa caccaagtag agttgaaaga gcaataagac atgcaataga
    37801 ggttgcatgg agtagaggaa aagttgacac aataaatcaa ttatttggat atacggtaca
    37861 caatactaaa ggaaaaccaa ctaattcaga atttatagca atgattgctg ataaattaag
    37921 actagaacat agtatggtta aataaacaag acataaaaag taaggctttt taattaaggc
    37981 attggctata aatgcgtatt acaagcagcg aaacggtata accactaggg ttataccgtt
    38041 tcgctatttt aataaatata aaaattttct ttattatttg catactatat caatataata
    38101 attttattat actatggata tagtatgtgt ctttacaagt tgtaaactga cagtggttta
    38161 ttttttaata taaatattga ctttgatgca ggtaaacttt gtatttttaa gcgtattatg
    38221 gaatatgtta aataaaaaaa tgatgaaata tagtattgta aatgccaaag atgcaaaaca
    38281 aacttaaaac atttatttta ttgttaagta atgctataat ataatgtgat tttaataatg
    38341 atagtggagg tttaaatatg agagtcgagg cccctataaa agtagatcga aaaaccaaaa
    38401 aacttgctaa aagagttgaa agtggggaaa tagcagttat aaatcatata gacatagatg
    38461 aagttgctgc aaactcttta gtagaagcta aaataaaact tgtcataaat gcggctcctt
    38521 ctataagtgg taggtatccc aataaaggtc cagggatatt aactgaaaat aatatactaa
    38581 taattgataa tgttggagaa gagttatttg aagaattaaa agaaggagaa actatagaag
    38641 ttgtagatgg aaagatatat agaaaaggaa aatttttagg tgcaggagaa gttttagaca
    38701 agtatgaggt ttcacaccaa ataaaggctg cctatgagaa tttagctgta gaattggata
    38761 gatttataga taatactata gattatgcta aaaaagaaaa aggatttata cttggagaag
    38821 tagaaattcc aaaagtgaaa actaattatg ctaataaaca tgtattgata gttgtaagag
    38881 gtcaggacta caaagaagat ttaagtacta tgctttctta tatagaagag gtaaagccag
    38941 tattggttgg tgttgatgga ggagcagacg ctttaattga gtttggatat actccagatg
    39001 tgattgtagg tgatatggat agtgtaagtg atgaagcttt aaaaaaggct agtgaaatag
    39061 tagtacatgc ttatacagat ggaagagcac caggtcttaa gagggttgag gaattaggct
    39121 tagatgcagt tgtatttcct gcgccaggaa ctagtgaaga tatagctatg cttatcgctt
    39181 atgagtataa ggctgagtta atagtagcag ttggtacaca ttcaaatatg attgattttc
    39241 ttgaaaaagg aagaaaaggt atggcaagta cttttttagt tagactaaaa ataggttcaa
    39301 agcttataga tgctaaaggc gttaatttgt tatacagaag taaattaaaa ataaagtaca
    39361 tatgggcatt aatagcaaca gcattatttc ctgtgttggt agttgcatca ttgtcaccag
    39421 gagttcaaca gttcatacaa ctaatgcaat taaagtttag agtattatta cagatgtaaa
    39481 ttttaggagg ataaatatgc atattaatat gaaatattat atagtcacta taggggctat
    39541 atttatagct ttgggtatag gcatgttagt aggatttaat ttaaataata atcaacaatt
    39601 aagtgaacaa caagcaaaca taataaatga tttggatgat aagtttgata tcttaaaaga
    39661 aaaaaatgat aaacttgaca gtgacttagc aagtgtaaat agagattatg aagaggcagt
    39721 taattttata aataaaaatg tagataagat tttagctggt tcgctgaatg gtaagagtat
    39781 aggaataata tctgctaatg aaaatgacga ttatacaaaa aatatagaag acataataaa
    39841 taaatctaat ggaagcattg catttaatat aatattaaaa gaaaacataa caaatccaga
    39901 aaagttaaag gaaatgtcaa ctaaactagg tacagatgta aaaaatgcaa atgatgcagt
    39961 taattatata attgacactc ttaaaaagga agatgcaagt gacattttaa catatcttca
    40021 agaactagat gtaataaagt ttaattttat tggagataca tatctaaaat atgattccgt
    40081 tgtaatagct ggtggaaatg atgcaaaaga cagtacaaaa cagtttgaaa aaatagaaaa
    40141 atttgtagtt tcaaaactta aatcagagaa taaatattta gtagaagttc aaaatacagg
    40201 agttaaaact tcttatgtag aactatattc taaaaacaaa gttgcaacta tagacaatat
    40261 tgatgaaggt ataggaagta tatctctagc gattcttcta caacaaggta atatagttgg
    40321 taattttggt agattggata ctgcaacttc attgttgcca tctattaaat agaaataatt
    40381 ttaaggagag aaaaaatgga ttcttatata agtataataa taccagctta taatgaagaa
    40441 agtagaataa aatatacatt agaaagcata ttggacatta agcaaataaa tgaaataata
    40501 gttgtggatg atggttcaat tgacaatact gctaaggttg tatctgagct agaaaatgaa
    40561 aagattaaat tttttaagtt agataaaaat agaggtaaag gatatgcttt aaattatggt
    40621 atcaaattag caatgaaaaa tgcagatatt ataggttttc tggatggaga tttaggaagt
    40681 tctgctaaag aagttgaaaa attaatagta cctatattaa atggtgaggc ggatgttaca
    40741 atagccaagt ttcctcctgc aaagagaaaa ggaggtttgg gatttgtaaa aggtttagct
    40801 aaaaattctg tgtttgagat gactggtgtt gagttagatg caactttgtc aggtcaaaga
    40861 atatttaaaa gagaagtttt agaaaagttt gatgaaatac cttttggata tggagtagaa
    40921 gttggaatga ccattgatat attaaagtat ggatttaaaa ttaaagaagt attagtaaat
    40981 atgactcata atgaaacagg aagaaattta aaaggtttta ttcatagagg gaaacagtat
    41041 tatcacataa aaaaggtttt aggacaaaaa aagaaagaat ggaggtaata tcttagtgaa
    41101 agattatata ctttatacca ttttattatt aactggatta tttggaacct atgctgtaat
    41161 tccactattt aaaaatttat tgataaatgg aaatgtactt agaccaaact ataaaaaaga
    41221 tatgatacca gtaagtatgg gaattgtatt tttgcctatg attgtaatca atggaataat
    41281 acttgctttc tttactacag aatttaagga tttagcgtat atatttatgt ttatgtttgg
    41341 tatgatatcc atgttttttg cagggatact agatgatgtc attggaaata gagatgtaag
    41401 tggtttaaag gggcatttta aaagtttatt taaaggaagt cttacaacag gaggttttaa
    41461 agctttattt ggtggatttg tagggcttat agtttctgtt gctatatcaa aagacataat
    41521 tgatataata gtaaatacac taataatagc tctatcaact aatttaatga atcttttgga
    41581 tttaagacca ggaagggcaa taaaagcata tttattcata atggtaatta tatttttaac
    41641 attgacagga tttgttcaag ttcttccatt acttatagta ccaaatgtac ttgcatactt
    41701 taattatgat ttaaaagcaa gggctatgat gggagatact ggttcaaacg tccttggaat
    41761 atctattgga atgctcattt cttttgggta tccatttaac attagattag gatggttaat
    41821 atttgtagtt attatgcata tattcactga gaaatactca ttaactaaga taatagaaaa
    41881 aaataaacta ttaaatttta tagataaatt gggaaggtag tattatgata agcaaaagag
    41941 tggggattgt tgaatcaata gtaagtcaaa ctgaaactct agatgatata agagtaaata
    42001 taaatggaga aatacaaaag gcatataatt atccaaaaat atctggaact ataaatatag
    42061 gtgacgaagt ggttttaaat acaacagcag tagaactgag cttaggtaca ggtggatacc
    42121 attttgtaat aactaattta aataatatag aatctacttt aactgaaggt ggacatataa
    42181 tgaaacttag atatacgcct cttcaaataa aagtggattc tgttgaggaa caagaaagta
    42241 tttatcatga taaatttgcg aattttaaag gattagaagg tctttctgtt gtagtaggga
    42301 ctcttcatag tatgttaact ccatttgcag cttcatttaa aagaaataat ccaaataaaa
    42361 aattagtgta tataatgact gacggagcat ctcttccaat ttatttaagt aaaaatgtgg
    42421 atactttaaa agaaaaaaaa ttaatagata gtacaataac tataggaaat gcgtttggtg
    42481 gagattatga atgtataaat atatatactg cattaattac agcaaaagaa atactaaaag
    42541 cggatgcagt atttgtcagt atgggacctg gaattgctgg gacaggaaca aaatatggat
    42601 ttacagggat tgaacaaggc tctattcttg atgccgtaaa aaaacttgaa ggaagagcaa
    42661 ttgcaattcc aagaattagt tttgctgaca aacgagaaag acatcaaggt atttcacatc
    42721 atagtatgac tgtatttaaa gaaattgtaa atgtaaatgt agacatacca ataactatat
    42781 atgatgatga aaaattgaac tttataaaag agcaaatcaa tttaaatggt ttagacaaaa
    42841 agcataatat aatttatata gataataata aaacgaaaga ggatttagac tattttaatc
    42901 taaaagtaaa gagtatggga agaaattatg aacaagatga ggcatttttc aaagctgcaa
    42961 gcacagcagc atattactta atggaggttt aagatggtat tagaagaaaa aactattagt
    43021 agtgataggg tttatacagg taaagtaatt actttaaagg ttgatactgt tgaaattcca
    43081 gggcaaggat atcaaaaaag ggagttagta gaagttggtg gagcagtagg aatagttgca
    43141 atcacagatg ataataaagt tgtgttagtt aagcaattta gaaaacctat agaaaagcca
    43201 atctttgaaa ttccagctgg taaactagaa aaaaatgaaa gtccaaaaga atgtgctgaa
    43261 agagaattaa aagaagaaac tgggtatagt gcaaaaaata ttaagctaat acataaattt
    43321 ttcacttcag caggtttttc taatgagata atgtttgtat atttagcaac aggtcttact
    43381 ccaggagaga ataatttaga tgcagatgag tttttagatg tctatgaaat tgagcttgaa
    43441 gaagcatata acatggtatt aaaaaatgac gttgaagatg caaaaacatc tattggatta
    43501 ttattagtaa aagatatgtt taaaaattaa tatttataaa atgaataacc ctttgtccta
    43561 aagcatatta ttttataaag gacaaggggg aattaaattg agaagaacat atagaaaaaa
    43621 tgattttaga gaattaaata gacaaataat tattatagga ttgttgttta tgatgtctat
    43681 tgctgttggg tcttacataa ataaaatatt gccaggttct agtaacaata tattaaacaa
    43741 tataaatcct gctgtagagt attataatct aaatataagc ataaaagata cagtgataca
    43801 aaatttaaaa tcagatgcaa tctttatggg ctcaatagct ctattaagtc tatttgtagt
    43861 aacaattcca gcagtgttgg tagcttttgt attaaaaggg atgtcaattg gttatacaat
    43921 aaatagttgt atattggcat taaaatttaa aagtataaaa atgattttaa taatattatt
    43981 taaaaatttg attattattc catgtgcaat catattagca ttaattagtt taagttattt
    44041 taaagaaatg gtttatgaat ttaaaaagaa aaatagaaaa aatatgcaat ttttaattaa
    44101 gaggtatata ctaaatatta taattataat agcactatca ttggggttgc aattaatatt
    44161 aaatactgca agtattggta ttattaagtt cctagctaaa taaaggagtt tataatggat
    44221 attatagagg gatatataga ttacataaaa gataaaaaaa aattatcaga gaatacagtg
    44281 gcatcatact ttatggatat aaaaaaatat atggaatata taaatcaaaa aagcattaaa
    44341 ctagtagata tagtagaaaa tgatattata agttacttaa taaagttgga aaaagataat
    44401 gtatcaatag ctacaatagc gaggatgata tcctctataa aatcttttca tgattattta
    44461 tttttaaatc atatatgtac aaataatcct gcaaaggata taaaaaaacc taaaataaaa
    44521 aaagaaaata taaatatact tacagaagaa gaaattgaaa gactattaaa ttttccaaag
    44581 ttaacaacgc ctaaattgat tagggacaag gctatttttg aagtattata tggcactggt
    44641 ataaaagtat ctgaattagt agagatgaat atagaggata ttgacctaga tattgattat
    44701 atatattgta attcatgttg taaaaatcaa agagtgatac cactttgtga tattacaaaa
    44761 ctttacttag aaaagtatct aaaagaagca agacctaaaa tggctgtaga aggtgaaaaa
    44821 tcattattcg taagttcatt aggtcaaagg tttacacgac aaggtctttg gaaagtcata
    44881 aaaaaatatt ctaatctagc caatatagat aaaaatataa atcctacaat gcttagacat
    44941 tcatttgcta ttcatttgtt aaatgaaggt gctaacattg ctgttgtaag taaaatttta
    45001 ggaaatgtta atttatctag tttgcaagta tatttaaatc atatagataa aaacgtgaga
    45061 agagaaataa aggagaaaca tcctagaaat gatgttgatg tagaattaca aaaagcagaa
    45121 gctaaataga ctatatagca aattttataa tttagaagga gcatatttat atgagcagag
    45181 taatttggat tgtaattgat agtgttggga taggagcatt acctgatgct gaaaaatttg
    45241 gtgatagtaa ggatgtaagt acccttggaa atatatttaa ggaatatcca gatattcaga
    45301 ttcctaatat gagaaattta ggtataggaa acatagatgg tatagatttt tttgaaagta
    45361 taaaagaacc aataggttgt tttggaaagt gtaaggaaat gtctcaggga aaagatacta
    45421 ctacaggtca ttgggaaatg actggtataa tagttgacaa accatttaag acatttgaac
    45481 atggattctc taaggagata atagaagagt ttgagaaaaa aactggaaga aaagtagtag
    45541 gaaataaacc agcttcagga actgtaataa tagatgagta tggagagcat caaataaaaa
    45601 ctggagaggt aatagtgtat acatcagctg atagtgtatt tcagattgct gctaatgaag
    45661 aagtaattcc cctagaagaa ctctataata tgtgcaaaat cgctagggaa ataatgatgg
    45721 gggataatgc agtagctaga gtaattgcta gaccattcat aggtaagaaa aaaggtgagt
    45781 ttgtgaggac ttcaaataga agagattatt cactagaccc atttgaacca acagttcttg
    45841 ataatataaa agaatcagga cttgatgttt tagcagttgg aaaaatagaa gatattttta
    45901 atggaaaagg aattacagat gctatccaca ctaaaagcaa tatggatgga gtggatgaaa
    45961 ctttaaatta tatgaaacaa gataataaag gtctaatata ttcaaatctc gttgattttg
    46021 attctaaata tggtcatagg agagacccag aaggatataa gaaagctctt gaagagtttg
    46081 atagtagact tcctgaaata atggctaata tgagagaaga tgatatttta ataatcaatg
    46141 cagaccatgg aaatgaccca acatataaag gtacagacca tacaagagaa tatatacctg
    46201 ttatgattta tggcaataag attaaaaaag gttttaattt aggagtaaaa gatacttttg
    46261 ctgatattgg agcaactgtt gcagatatac taaatgtaaa gttgcctaaa catgggtcaa
    46321 gctttaaaga agatttattt taaactataa aatgataaaa acttagctta ataggatgga
    46381 gagattggaa tgtttgaaaa gatagcacaa agtagtaaat ttataaattc taaaagtaat
    46441 ataaaaccta agattggttt gatattaggt tctggactag gtgatttagc aaatgatata
    46501 gaagaccctg tcacaattaa atatagtgaa attccaaact tccctgtatc aacagtagca
    46561 ggacatgctg gacaacttgt aattggaaaa cttgaaggaa aagaagttat tgctatgcaa
    46621 ggtagatttc attactatga aggatattct cagaaagaag ctacttttcc agtaagggtt
    46681 atgaaagaat taggagtcga gattcttata gtaactaacg ctgctggagg cgtaaataaa
    46741 gaatttaaag caggagattt gatgcttata agagaccata taaactttag tggaagtaat
    46801 ccattagtag gaaaaaatga tgacagattt ggtgctagat ttccagatat gtcagatgca
    46861 tattcttcta aatatgtaga tgtagttaaa aactgcgcta aagaatgtaa tatagatgtc
    46921 aaagaaggcg tttatatgtt ttttagtggt ccaaactatg aaacaccagc agaagtgaga
    46981 atggcacaaa ttttgggagc tgatgctgta ggtatgtcta ctgttccaga agtcatagtg
    47041 gcttctcact ctaatatagg agttattggt atatcttgta taacaaatat ggctgctggc
    47101 atcttaaatc aaccactaag tcatgaggaa gtaatagaaa ctactcaaaa agttaaatca
    47161 gaatttatga atttagttaa aacaacggtt aaaaatttat aagttaaagc ttattataga
    47221 ttttttgagg aagtttataa taaaatagtt aatgtttaat ttgaaaagaa ttaataaaaa
    47281 gctgtcttga tatagaaagt tatttctaat aaaggacagt ttttttataa tactacaaca
    47341 tttgaaagtg ttttaaagtt taatatttat ttaaattatt ataggttaga atggagattt
    47401 caattatgag aatgtatgat attataaaaa aaaagagaga taacctagag cttagtaaaa
    47461 aagagataaa ctactttata gaaaactatt caaagggaga tatacctgac tatcaagctt
    47521 ctgcactatt aatgtcgatt tatttaaata agatgaataa acaggaaact gtacatctta
    47581 cagaagcaat gatgaactct ggagacatga taaacctatc tgagatagat ggaataaagg
    47641 tagataagca tagtactggt ggtgttggag ataaaactac catagctctt atacctttgg
    47701 ttgcttcatg tggagcacca gttgcaaaga tgtcaggtag aggtcttggt catactggtg
    47761 gtactattga taaattggag tctataccag gtttttcgac agaaatggag ataagtaaat
    47821 ttattgattc tgtaaataag tctaaaatag cagtttgtgg tcaaagtgca aaggtagcaa
    47881 ttgcagataa gaaaatatat gctcttagag atgttacagc aactgttgat aatatatcat
    47941 tgatatcatc aagtgtaatg tctaaaaagt tagcttcagg agctgatgca attgtgctag
    48001 atgtaaaaac tggtgatgga gcatttatga agacattaga agagtctttt gagttagcta
    48061 aatctatggt tgatataggt actggtatga atagagatac aatagggatt gtaacagata
    48121 tggatgaacc tttaggtttt gcagtgggaa actctttaga agttatagag gcaattgaga
    48181 cattaaaagg aaatggacct aaagaccttg ttgagctatg cgaagtatta ggagcatata
    48241 tgttggtttt atcaaaggta gcacatgatt ttgaagatgg tgttgaaaaa ataagagaat
    48301 caataaaatc tggtactgct atacaaaaac tcaaagagtt tatagagaat caaggtggta
    48361 ataaagatgt agtagatgat tattctttat tcaaaaaatc aaagtataaa tatccagtta
    48421 aatcaactaa atcaggttat ataagtaaaa ttaaagctga agatgttggt gtatcggcta
    48481 tgattcttgg agcaggtaga gaaactaaag atgatatttt agatttatca gcagggataa
    48541 ttttagaaaa gaaagtagga gattatgtaa atgaaggtga agttttagct tacatgtatt
    48601 ataatgatga acaaaagctt tcattggcaa agggaagatt tattgattcc tactctattg
    48661 tagattcaaa aatagaaaaa aataaattaa tatatggtat tgtaactaaa gaagagatta
    48721 aaaagtttta gaaaatttta gaaaagtgat tataaaaggc ctaacatgtc aaaaataatg
    48781 ttaggttttt tattttttga taaactataa attataccaa aaatatatat tttaaa
//
LOCUS       DACXMZ010000004        46965 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.004, whole genome shotgun sequence.
ACCESSION   DACXMZ010000004 DACXMZ010000000
VERSION     DACXMZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.004"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(110..298)
                     /locus_tag="JD016_00905"
     CDS             complement(110..298)
                     /locus_tag="JD016_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HAU4998184.1"
                     /translation="MAHMDFAVNDLEEAVQYAIHCGATIAEEQFTDDWRVMIDPAGHP
                     FCLCQMKSIMESSHFSLL"
     gene            complement(389..850)
                     /locus_tag="JD016_00910"
     CDS             complement(389..850)
                     /locus_tag="JD016_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HAU4998185.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            complement(888..1721)
                     /locus_tag="JD016_00915"
     CDS             complement(888..1721)
                     /locus_tag="JD016_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4998186.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            complement(1995..2681)
                     /locus_tag="JD016_00920"
     CDS             complement(1995..2681)
                     /locus_tag="JD016_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4998187.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKDVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNEADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TIIVSDGLIV"
     gene            complement(2696..5173)
                     /locus_tag="JD016_00925"
     CDS             complement(2696..5173)
                     /locus_tag="JD016_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAU4998188.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRILIESEEEITENL
                     VNKLSKELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILFPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(5392..6795)
                     /locus_tag="JD016_00930"
     CDS             complement(5392..6795)
                     /locus_tag="JD016_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU4998189.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHVTLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            7294..8154
                     /locus_tag="JD016_00935"
     CDS             7294..8154
                     /locus_tag="JD016_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAU4998190.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTVLDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(8393..8950)
                     /locus_tag="JD016_00940"
     CDS             complement(8393..8950)
                     /locus_tag="JD016_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU4998191.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(9140..10027)
                     /locus_tag="JD016_00945"
     CDS             complement(9140..10027)
                     /locus_tag="JD016_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAU4998192.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            10146..11345
                     /locus_tag="JD016_00950"
     CDS             10146..11345
                     /locus_tag="JD016_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-0--gamma-glutamyl ligase"
                     /protein_id="HAU4998193.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(11539..14094)
                     /locus_tag="JD016_00955"
     CDS             complement(11539..14094)
                     /locus_tag="JD016_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAU4998194.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGITKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(14091..14777)
                     /locus_tag="JD016_00960"
     CDS             complement(14091..14777)
                     /locus_tag="JD016_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4998195.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            complement(14854..15762)
                     /locus_tag="JD016_00965"
     CDS             complement(14854..15762)
                     /locus_tag="JD016_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU4998196.1"
                     /translation="MFILAFIICTVVIFIVSTKINQKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(15764..16435)
                     /locus_tag="JD016_00970"
     CDS             complement(15764..16435)
                     /locus_tag="JD016_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4998197.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            16724..17761
                     /locus_tag="JD016_00975"
     CDS             16724..17761
                     /locus_tag="JD016_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HAU4998198.1"
                     /translation="MFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRPNLCSEGTMT
                     YNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQYGASVVGIT
                     KLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICEVIETSKCYV
                     DASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAILTNKDYGSR
                     LRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGDDGKYNWVIE
                     HETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALDEYTSKFGKR
                     IFIPGNPSWLK"
     gene            17919..18605
                     /locus_tag="JD016_00980"
     CDS             17919..18605
                     /locus_tag="JD016_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4998199.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKGNYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKVKAFLNHLTVKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            18595..19626
                     /locus_tag="JD016_00985"
     CDS             18595..19626
                     /locus_tag="JD016_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU4998200.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALTIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            19708..20373
                     /locus_tag="JD016_00990"
     CDS             19708..20373
                     /locus_tag="JD016_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4998201.1"
                     /translation="MSILQVINLKKYYEQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            20386..22803
                     /locus_tag="JD016_00995"
     CDS             20386..22803
                     /locus_tag="JD016_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4998202.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VFFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMEIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKNVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(22984..23775)
                     /locus_tag="JD016_01000"
     CDS             complement(22984..23775)
                     /locus_tag="JD016_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAU4998203.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(23808..24395)
                     /locus_tag="JD016_01005"
     CDS             complement(23808..24395)
                     /locus_tag="JD016_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAU4998204.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARFFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKSDWNLSI"
     gene            complement(24572..25963)
                     /locus_tag="JD016_01010"
     CDS             complement(24572..25963)
                     /locus_tag="JD016_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998205.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(26147..27451)
                     /locus_tag="JD016_01015"
     CDS             complement(26147..27451)
                     /locus_tag="JD016_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAU4998206.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNNYY"
     gene            complement(27808..28683)
                     /locus_tag="JD016_01020"
     CDS             complement(27808..28683)
                     /locus_tag="JD016_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAU4998207.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(28876..29562)
                     /locus_tag="JD016_01025"
     CDS             complement(28876..29562)
                     /locus_tag="JD016_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAU4998208.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(29611..30162)
                     /locus_tag="JD016_01030"
     CDS             complement(29611..30162)
                     /locus_tag="JD016_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAU4998209.1"
                     /translation="MFKEKSMSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEH
                     EAVYTAEQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSR
                     LSFASPERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTV
                     NISRKGFEEFLEYLKYPIQSIDV"
     gene            complement(30188..30820)
                     /locus_tag="JD016_01035"
     CDS             complement(30188..30820)
                     /locus_tag="JD016_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HAU4998210.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(30910..31989)
                     /locus_tag="JD016_01040"
     CDS             complement(30910..31989)
                     /locus_tag="JD016_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998211.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(32122..33468)
                     /gene="pepV"
                     /locus_tag="JD016_01045"
     CDS             complement(32122..33468)
                     /gene="pepV"
                     /locus_tag="JD016_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAU4998212.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(33537..34817)
                     /locus_tag="JD016_01050"
     CDS             complement(33537..34817)
                     /locus_tag="JD016_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998213.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(34944..35204)
                     /gene="hfq"
                     /locus_tag="JD016_01055"
     CDS             complement(34944..35204)
                     /gene="hfq"
                     /locus_tag="JD016_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAU4998214.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(35318..36253)
                     /gene="miaA"
                     /locus_tag="JD016_01060"
     CDS             complement(35318..36253)
                     /gene="miaA"
                     /locus_tag="JD016_01060"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAU4998215.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEKEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINKKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(36291..38258)
                     /gene="mutL"
                     /locus_tag="JD016_01065"
     CDS             complement(36291..38258)
                     /gene="mutL"
                     /locus_tag="JD016_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAU4998216.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(38269..41112)
                     /gene="mutS"
                     /locus_tag="JD016_01070"
     CDS             complement(38269..41112)
                     /gene="mutS"
                     /locus_tag="JD016_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAU4998217.1"
                     /translation="MQIDINKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHDLIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(41273..42010)
                     /locus_tag="JD016_01075"
     CDS             complement(41273..42010)
                     /locus_tag="JD016_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4998218.1"
                     /translation="MMLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFV
                     SILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLI
                     LKKEKKSKARSMVKSYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDE
                     PFGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKE
                     FVIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(41976..42989)
                     /locus_tag="JD016_01080"
     CDS             complement(41976..42989)
                     /locus_tag="JD016_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAU4998219.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(43011..43781)
                     /locus_tag="JD016_01085"
     CDS             complement(43011..43781)
                     /locus_tag="JD016_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4998220.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(43756..44046)
                     /locus_tag="JD016_01090"
     CDS             complement(43756..44046)
                     /locus_tag="JD016_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HAU4998221.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(44177..44276)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      44896..44985
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            45038..45214
                     /locus_tag="JD016_01095"
     CDS             45038..45214
                     /locus_tag="JD016_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998222.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(45329..45685)
                     /locus_tag="JD016_01100"
     CDS             complement(45329..45685)
                     /locus_tag="JD016_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4998223.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            45941..46855
                     /locus_tag="JD016_01105"
     CDS             45941..46855
                     /locus_tag="JD016_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HAU4998224.1"
                     /translation="MNKKFRLYQVDSFTKEKFTGNPAGVITNADGLSESQMQKIAREL
                     NNSETAFIFNNLAKNHQENYDVHIRFFTPTNEVPICGHATIAAHYVRAVENQFNNKTV
                     FQKTGAGILPVDIIKKDNDYEIIMTQGKIEVSPPLQQTYQKRLLNAFGILKEDIRTDC
                     PIAISSTGHSKVMIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHNKETLQVH
                     GRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPTPNTEFIFEAVQGEAIKRKGKMLV
                     KVIILDNKPVEVKIIGKAVIAFSTNIEI"
BASE COUNT        16083 a         7279 c         5398 g        18205 t
ORIGIN      
        1 ttgataaagc tctactattt atatttttta gtgtattcat gtataataag taaaagcaca
       61 acggtatata ctgttgtgct tttttgtaat taataaacaa tattttggtc tataacaatg
      121 aaaaatggct actttccata attgatttca tctgacataa gcaaaaaggg tgtccagctg
      181 ggtcaatcat aactctccag tcatcagtaa attgctcctc tgcgattgta gctccacaat
      241 gaattgcata ttgaactgct tcttctaaat cattaacagc gaaatccata tgagccattt
      301 gctgttgaga ttcaggtttt tctggttcta ttatgcccca agcctttgtt ttttgtaata
      361 taaagaaaaa attatatatt ttaatatttt ataattgttg aaattgtgta ataaattcaa
      421 ctggatacat agtgcgtttt gaaatttctt ctaaagctaa accttgtttt aaaagcctct
      481 ttatgacgct ttccatactt tctgaaattt ctataatatt tttatcaaaa tcataaaacc
      541 taaaagttct ttgaccccat gggtattctt ttatataatg caaaaattca atatttccta
      601 ttctatttat cttactttcc cattgtttga tgtcattaac ctcaaaatac aactgaaagt
      661 tatttgattg ttgatatatt ggtaaatcat atccaattat agctttataa ttactttgta
      721 atccaaatcc attttcaaat gatacatgtt ctcctaaatc taatgtgatt ttttgctcca
      781 taacatcttc ataaaacttt tttgatttat ttatgtcttt gacaattagc aatactcctc
      841 caaatttcat cttatcacta tcctttcttt gttataagta ttataactta aaattttgca
      901 atttcactgt aaaaatcaga catattagct cggtattttt gaggagttat tccacagaca
      961 attttaaaat ctttaataaa atgtggtata tcatagaatc ctaattcatt acatatagac
     1021 atcaatgaag ttttattatt gtttagaagt tttatagatt tattaatacg taccaaacga
     1081 gaaaatgact tcatactcaa ccctaggtac tgattaaaga gtctgtttaa atgcctagaa
     1141 ctataaaata cttttttaga aatttcttgt gaagatatat ttccagagtt ttgaataatc
     1201 aactgaattg ccaaaataaa ctcatatgag tattcaattt taatattaga aatcaataat
     1261 tcttccaact ttaaaatcaa tttatctata gataaagatt cattaaatat ttttttaatt
     1321 tctaaatcta atgaagtatt aattatagaa aatggaacaa ctttatcaat aagttcactt
     1381 tgctgtatac ctataagcgg gaaaaaacca gcaggttgaa attctataat tagtataaca
     1441 tcacatctat tagcaatatt tcctactgtt tttggtttag tagttggacc aaataataga
     1501 ctatgtaacc ctgtggcatc ataaaaaaac accaaagtta cacttccatg aggaataatc
     1561 gtgtaattat cagaaattat tgtttgattt ggaaacgata tagtaaaatt cgatatccaa
     1621 tttcttaatt gtatacaagg caagagataa acaaaattgt cttcacaaac tataagtgac
     1681 ctatttttat ttataaaatg aatactttct ttttttaaca tatattatat cctgtcctat
     1741 cgtgaatatt tattaaagta agaagaatcc atattatctc tatatatcat taactgcttt
     1801 taataataga gatagtatga atactcatta gatatttaat aaacaaataa aattaaaata
     1861 atcaataaaa aatatagtac ttttatctgt tattttaata ttatagcaca cttatagtat
     1921 ttaacttata tccactttac atatgtctaa tttatataat taaaaatgct tatctcaaat
     1981 acaataatta gtccttatac aattaaacca tctgaaacta taatagtacg actactactt
     2041 ttggctgctt ctggtgagtg agttaccata attatagttt gtccattgtc tctgtttatt
     2101 ttattcagta aatctattat ttctatccct gttttactat ctaaatttcc agtaggctca
     2161 tcagcaaata aaatctctgg ttttccaatt aaagctcgag ctatagcaac acgttgttgc
     2221 tgaccaccag ataactctcg tggtgtatgt ttacgtctat cagttaaacc aactatatct
     2281 aagatttcat ctaattgatt tttataatct tttaaatttt taccatctaa taacaaagga
     2341 agcattatat tttcctccac atttaaattt ggtattaagt tataaaattg aaatacgaat
     2401 cctatatttc tacgacgtat tatgctcatt ttctcatcat taaaatgtga tatatcagct
     2461 tcattcatat acacttttcc gctcgttgga gtatctaaac cacctaaaat atataaaaga
     2521 gtacttttgc cagaaccaga ttgacccatg atagaaacaa attcaccttt cattacttgt
     2581 aaagatacat cttttaacac tcgagttgtt gtatttccaa ttttatattc tttgataata
     2641 ttttttgctt ctacagctaa actattatgt gtaattttca tattaacctc cataattatt
     2701 caaacttaat ttcttctatc agtttcattt tcttcccttt tataataggc actatcgaac
     2761 ctattaaagt tactataatt cccaataacc caacaataat aaatgtttta aaatctaaat
     2821 ctggtttcat tgaaattttt ggtcctgcaa ctataaagat agtttgaatc tcaagtatag
     2881 atatcacaat accaataaca gctccaagta aacctgaagt aaacccttca atcaatgtta
     2941 cttttatatt ttgtttatca cttagtccta tagatttata catagctata gaacgtcttt
     3001 tttgaatata attaatcagt agattattaa taatacctac tgtagcaaga agaaaaatga
     3061 aataggtcat cttattcata ggagacaaaa aactacttat agtatttaaa gaatcattat
     3121 taaattcttc aactgtacga ctccaattat atgtatctcc aaataaatat cttatctgaa
     3181 tcataacagc atctggattg actgctgtat atactaaaaa accataatta gttttatcaa
     3241 aatcattaac tgcataatgt gatggtataa cagcttctac atcatttgca cgggatttaa
     3301 aactacctac tattttataa gaaaaatcat acttatcatt agatagagtt attgtatctc
     3361 ctatagataa gcctactttt tttagagtat cttcattcaa aataacagaa cgtttatcat
     3421 taaaagcttc tattacttgt ttttttatct caaaatttgt ataattgatt ccaaacatag
     3481 agttatatag ttttatatta tctgtaccct ctagtcttga taatgttaca ccattaccag
     3541 aaatctcatt attcataaca taaagaggta atattttttt aattccatcc atatgtctaa
     3601 catcttcaat aaactcttca ttcatctttc catctgcaaa accttgtaat tctgcgtcac
     3661 gaaacacatc tgttatataa gttttgacaa agttaccaac aacacttatt gcaataactg
     3721 cagaaatact tataaatagt aaagtgatat tctgtattat atttttgtta tttttcatat
     3781 ttctagcagc taatttaccc tcattcccta aaatattttt ataaacaaac tcaaaaacta
     3841 tagacatgat atcagtaata agaggaatca acacaatagt agctacaatt aatcccagta
     3901 atgaaaatcc accagctaga tacaatgtat tttcagggga tattcttgga aataaaatag
     3961 ataaaataaa cattatactt cctataaaaa gtatagaacg atttgaaaca ttcttttctt
     4021 ctactgttcc tagtacaata ttttttattg gcaaatggct agctttttta acaggtatat
     4081 atgcactaaa tgaagaaacg attattgcta ctataacaga tataatcact ccaatgggtg
     4141 atattactac tggaattgat attccttgtt cgagagaatt tcctaaccca tgaagcatta
     4201 aatttaaaac aactacacca ataggaatag caattaatcc tcctatactt ccgtataata
     4261 tactttcaag catcaatata cgagttacag ttttttcatt ggcaccaata cttctaaatg
     4321 ttccaataaa aggaagacgt tctaatgtaa taaccttata actgctgtat attataaata
     4381 tactaagggt taatgcaaaa aaacttatta aaaagaaagg cattgtcttt tgtctagcat
     4441 ctgaaattat cttagtttca tttatagtat ttgataccat atatttttca gtagaaagtt
     4501 ctttggataa tttatttact agattttctg taatttcttc ttctgactct ataagaattc
     4561 ttgtatatcc actgccttta tttataattt ttgatagagt ctctttgggt agcagtgcat
     4621 ttacacctct tgtgttcctc aaaaatactg tatcataaga tgcaatatca ctaacttgaa
     4681 aagtataaga ctttccataa atctgcaaag taatagaatc accttttttt attttatatt
     4741 ttgatgtaaa gcgatttgga agaataattt tatcaccaga aaaatcagta atactatcgc
     4801 cattttctaa tcgtggcttg ttgattttat ttagttgact taagtctgct gatataagac
     4861 taaattcttc atagtatccc cctttattat aaatagcaga gctttccaat acaccaacct
     4921 ttgattttat agtatttaaa tcaggtatgt cttctaaatt ggttaatata tctggatttt
     4981 tactttggac agatatagtc gctgtaccat acataccttt tgccattttt ctttgtgcac
     5041 tttcataaga taatccaatt gagaatgaaa caaaaagtaa aactgttgat aaaactatgg
     5101 acaataacat aactgctgtt ctaatttttc gttctttaat atttgtgaaa atatacttta
     5161 atataatttt caaacttaca ccttcttccc ttatctaaga tatttcaatc taatattttt
     5221 atactttaat ctaaattaaa tctctaatag actcctccat acttttatca ttctatttat
     5281 aatattttta atcaaatcac cccttaaata aaaaagattg ataaactact atataaatat
     5341 aataatctat caaccttaat atatttttta tttaacctta attaacctta attaaatagg
     5401 aaaggtgata taaaatgagc tacctaatcc aggtatactt tcaacagata tattccctcc
     5461 atgaacttca ataatttgct tcgttattgc tagtcctaaa ccagttcctt ctattttttg
     5521 ttttgtattg cttcctctat aataacgatt aaacaaattt gatgcttctt ctcttgacat
     5581 cccttttcca ttatcagata taatgatttc aattctttca tttaatgtta catgtacaca
     5641 aactttagta tttttatctc catatgtaaa agcattgaca ataagattac taaaagctcg
     5701 ttttaatagt gtacaatcaa aatcaaagtt aatattatct atattactct taaattcaac
     5761 tatatttgat tcatattctg gataatttaa aatatcaatg gataattctt tcaagaatct
     5821 tactaaatca taattatttt tatttaaagg gattattcca ttttttaatt gataggttaa
     5881 ttttaaatcg tttataagtg tatttgtata ttctatgttc tttaacatga ttgaagcata
     5941 tttatatact ttttcaggtg aataattagt actactatct gtaagaagct ctgcataccc
     6001 ttttattggt gaaagtggag tttttaaatc atgagttata tttgaaatcc actcttctct
     6061 cataatatca gttttatttt gtatctcatc acttctatga atttcttcat ttaaaatatt
     6121 taaactatta tatatatcag caaaaacacc tttaatatta actggaagat agcttcgttt
     6181 tgaaatatcg ctaacagcag atgtaatatt tgatatgcac tttgttaccc agtaaccata
     6241 agcaattcca gaaaccaaaa ttattaaaaa tataatccct atgattgaaa atacaattga
     6301 cttaccacct ataaatttac ctccatcaaa aaacatattc actttagata tatttaatgg
     6361 aaaatatata atataaatat agtcattttt attattttta attgtattaa agtatacagt
     6421 tccattactt gaattatttt ggttagctaa tgctaataat tcagaattag aataataatc
     6481 tttatgctct tttggttcta aaaacccata aactctattt ccactagaat ctaaaatttg
     6541 caaccacaat tgattttctc gaagtttttc taatccagac tgttttactt gtggcttatt
     6601 atctataaat acgatttgtt cattaaagtt ttctgtaaat tttttcggcc aatcactacg
     6661 aataaattca ccatttggtt tctgaatagt aataagtgat aagaaaatac ctattactgt
     6721 caggattaag caaataaaaa tcatgaaaaa aataatgtat gaatgaaata ctgttttata
     6781 actaggttta tgcatttaat caactctttt cttaaacttt tattttagtt tgattgttgc
     6841 ttatataaaa tagacaataa ttattgatat gtaaaaattt atttaaaaat acaactatct
     6901 acataaatta tatttatata attatatgtt atcaagctgt ctgttgtatt taataaatca
     6961 ttttgataac atataaaagt aagataaatt tttccatcaa ataccttata attatacaca
     7021 aaatctctat atacgttaaa cagtatagag aaaattttca tagaaccatc tttatagggg
     7081 atactttata aaagaataaa aaaattataa tatataaaaa acttcatagg agtgtaacaa
     7141 catcagtaaa gatatgaaaa cactatataa aaattcaaaa atattaagaa taatgaaata
     7201 cttgaaattg cttatatcaa gaaattttat ataatataaa ctggtcaaat atgttataat
     7261 attaaattgt gacaagttca tggaggtgtt tatatgaaaa aacaaaagtc gccaatttta
     7321 cttgaaatgc ttggactatt ttttggttgt atatcaatga gtattggtat aaatatgttt
     7381 ttaaaaccac atacaattgc tcctggagga cttagtggtc tatcattagt actaaacaag
     7441 gttacaggac ttccagtatc agctatcatg ttaattatag gtgtcccatt agtaatactt
     7501 gcttttagaa ttatgggtac aaaaaattca ttaaaaactt tatttggaac agttgtattt
     7561 tcagctatag ttcaattaac agacccatta tcaaaactaa gatttactaa tgacttatta
     7621 ttgtcttcta ttgcaggagg aatcctagtt ggtataggtt taggaataat gtttaaatca
     7681 gatgcttcta ctggaggaac agacttaatt gcactcatac ttagtaagaa atttccagga
     7741 ataaaagcta caaaattcat gtcatgccta gatggaatgg ttgttatatc ttctggtata
     7801 gtaaatcgta gtttagaaac tgctctttac tctggtatag cactttgtgt attaataaaa
     7861 atagcagata tgataatgga aggatttgac cattcaaaag cattttttat tatctctgat
     7921 gagcctgaaa gtttaagaca agctataact gaagaacttg atagaggtct tactgttctt
     7981 gatggtaaag gtggatacac tagagaaaat aaagaagttc tattagtcgt agtttctaaa
     8041 aaacaagaac tttatctaaa gagattagtt aaaagaactg accctactgc atttgttatt
     8101 gttacagatg tacatgaggt tcttggagaa ggatttaaaa atttagagaa ctaataaaaa
     8161 ttaatatttt aagtatatat actattttaa ttaaaatacg atttattcaa aaagagtgtc
     8221 tcataaactt tttagttcat gagacactct tttttgtata aaataaaatg cattttcagt
     8281 attttaacaa taaaaacagg attatctata ttttgaggca accactttat tgaaatttta
     8341 tttagaaaac aatcattact aaataaccag catagatatt aaatgattat atctattgtt
     8401 ttttcaattc atattgataa caaggaacat tttttacagt atttccagta tgaatttttt
     8461 tgatatactt tcctgtacat ttaaatccta ataaatctaa tgattttgta gaattttggt
     8521 tttcttcata ggttattgca attattttat ttaagtttag ttctctaaat ccatactgta
     8581 ttactctttt tgttatctca gtagcaaaat catgtcccca atagcttgga tttatacata
     8641 tacctatttc tgcttctaat gctttaggat ttatattatt aaaccccata ttcccaataa
     8701 ctttattgtc tcttttatat actatagcaa agtggccaat tttccctttt ttatatggat
     8761 ttagaaaaaa tgattttata aactttcttg tatcttctaa agatttatgc tcactaaatg
     8821 gtaaatgttt tactactatt ttatttttat aacagtcaaa aaaatcttca acatcatcta
     8881 atgatgcagg tcttagtata caacttctag tttctaaggt tttcatttga ggatattcag
     8941 aatcagacat ttctaatccc cctttataca ttaatctaaa tatactgttc atatttaaaa
     9001 tacgatatta attatataaa tgttctcaaa ttaaccttat ttttattttt aataaaaact
     9061 aattactata tgaatctttc aagtttacga atttatctct atttttatat taaattactt
     9121 gtgaaatatg tattctaaat tatatattat tagatatcat gcttaaaaat tctttggttg
     9181 ctaaggataa agatatatct tttaaatggc agtaacctat atttctctta ggtattggct
     9241 cttttatatc taatttaaac aatttctcat tttccaaata atctatgcta aattcttcta
     9301 taacacaaga cactccaaga tttatatacg caaattctaa aagtaattca tgagaaccca
     9361 attctatatc cgggtttagt tttatacctt ttgataaaaa atattcattt acataaagtc
     9421 ttgaatttgc tttattttct agcattatta aaggcaatgt gtttaattct tccaatgata
     9481 ttttcctacc tttgtattct atgtaatcat ttgcacatac aaatatatca tgtatttcaa
     9541 tacactcaac aatatttagt gtatcatctt ctattggcat gtttataatt gctatatcta
     9601 tattcccatc tttgagtaaa gatataagtt ctctactcgt tctatttatt gtagatacat
     9661 gtatatgtgg atacttctta tgaaaaattt ctaaatattt taacaagaaa aaacgtgcag
     9721 ctgtatctcc tactcctatt ttcagagaac cgtattctaa ttttttaaat ttatttattc
     9781 tatcttctcc tgatgatatt aactttaatg cagtagtgac atgttcactt agaattttcc
     9841 cctctggagt taattttact cccttattgt ttctataaaa taataaagta tctaattgtt
     9901 cttctaattg ctttattgac tggcttacag ctggctgtga cataaacaat tccttagcag
     9961 caagagaaaa actcttatta tttacaactg cattaaaaac cttataaagt tcaagtttta
    10021 tattcatata taattactcc ttatgtcaaa tattagtatt atttatttta cttataatta
    10081 tatttgaatt ataatataca tggtactaat atgacaatat taattcttta ggaggatttt
    10141 agataatgga tagattagtt ggaactgttt caagaggggt tagagcacct attataagac
    10201 aaggagatga cttagttaaa atagttgttg attctgtttt aaatgcgtca aaaagtgaaa
    10261 actttgaagt tagggacaaa gatgtaattg ccgttacaga agctgttgta gctagagcac
    10321 agggaaatta tgctcacgtt gacaatattg caaaagatgt aaaagataaa tttggagatg
    10381 atactgttgg ggttatattc ccaattttaa gtagaaatcg ttttgctata tgtttaaaag
    10441 gaattgcaaa aggatgtaga aaagtcgtat taatgttaag ctacccttct gatgaagttg
    10501 gaaatcactt aataagtatt gatgaacttg atgataaggg aataaatcct tggtcagatg
    10561 ttttaacaga agaaaaatac agagaactat ttggatataa caagcataca tttactggtg
    10621 tagattatgt tgattattat aagaatttaa tcaaggattg tggtgcagag gtagaaatag
    10681 tatttgcaaa caatccaaaa actattctta actatacaac aagtatttta acttgtgata
    10741 tacatacaag acaaagaaca aagagaattt taagacaaaa tggagctaaa aaagtatatt
    10801 cacttgatga tataatgaca gcaagtgtag atggtagtgg ttacaatgac cagtatggac
    10861 ttttaggttc aaacaaagct acagaagaaa cagtaaaatt attccctata aattgtgatg
    10921 aagttgtaaa caaaatccaa ggtaatataa aagaaataac agggaaagat gtagaagtaa
    10981 tggtttatgg agatggagct tttaaagacc ctgtaggtaa aatatgggaa ttagctgacc
    11041 cagtagtatc tcctgcttat acaaaagggc tagagggaac tccaaacgaa gtaaaattaa
    11101 aatacttagc agataatgat tttgctgact tatctggtga tgagttaaaa gaagcaattt
    11161 caaaatacat agttgaaaaa gataataaat ctgatgattt aacaggaaac atggtttctc
    11221 aaggtactac tccaagaaga ttaacagacc ttataggttc tttagcagat ttaacatctg
    11281 gaagtggaga taagggtact cctattattt acatacaagg atatttcgat aactatacaa
    11341 aatagtatgt tttaataaga tttaaatttt ttggattata tatattaaca tgaaaattta
    11401 ttaaaacctc ttaatagata tgacttttca agagttatat ctattaagag gtttatttaa
    11461 tgttcacaga taagatatat ttaaataatt taaacaagat tgtcagaaag ttatataatc
    11521 ttaaaaattt atataactct aattcttatt tatttctgaa ataatatcac tcttcaaaat
    11581 ttgtcttact ggaataatta cagcaactat actaatagtg atatttaaaa taatcatacc
    11641 tatgtataga ggtgtattta ttttaagaat tggatttata tataaatata cacgttttac
    11701 cataaaataa atcataaatt gtccaataac acttccaatt accattataa tacttgcata
    11761 gaatccatat aaaaaacctt ctcttatcat cataagtaaa aacttattat ccgtaatacc
    11821 catagctctt aaaattccaa actcatgtct tcttgaaaaa attaagtagc ttactgtatt
    11881 gattatatgg aacaaactta ttgcaagaag tacaaataca ataccatata taaataagag
    11941 tttttgattt aagtaggtat tatttttttc aatagcaatg gtataatctg ttacaataca
    12001 tctttttaca tcacttacaa cactttttat gttctcagac aattcctcac tgttactatc
    12061 ttctgtcttt tttatactta ctgaattata attttcgata tcaaagttat ttttcatttg
    12121 accattcgtc ataactatat ccataccata atcaccaatg aaatatatgt tatttgccat
    12181 cacacgtttt acagtagcag caactacaaa ttcttttgtt gtataatcat tcttattttc
    12241 ttctttaaat ttaacaattt catctgttgg tttcattgtc tttggcactt taacttttat
    12301 tgtatctcct ggatttatat caactgcatc atacatacca gttccatcca taggtagtct
    12361 aactataacc ttattatttt taaccatatc atgggtatta atctccccat ctataatata
    12421 agaatttaat ccttctaaca taccattgtt ataaccataa atatttgttt ttaaaagata
    12481 gccaccttca ttttgctttg tacaaatacc atcaaaaaaa gtctttatcc tgtaatcttt
    12541 ttcaagaggt ttaaagaaat ttttccaaaa tagttgttct tctttaattt gcatagctcc
    12601 aaataaatat cttactggat agacttttga tattccctca acattgttta atcttgaaat
    12661 tttatctttt ggtataccta catcaaaatc tgttgtttgc atagttattt ggtagtcaga
    12721 atttaaacca tcatcagctt tcattgtaag ttcattatta tttttagtaa gctgtataga
    12781 gtaactagaa catagaaata taattccacc aagtgataat gaaaacaaaa ttccaataaa
    12841 cattcccttt ttttgagtca tgtatttatg agataatact tttgtcatgt tagagtgttt
    12901 tacactttct aatattctct ttttcttatt agtaatctta aagtttttca tagattctat
    12961 actagagtat ttattcaact gatgtacaac acgtacagca attaaaatta acaacaataa
    13021 gataaataaa aagcctaaat aaatagattt ttcacttata aaaaatgctc ctggaacaat
    13081 atcactactc aaaaatatat gtggaaattg ttcatatatt gtagacgcta ctccaatacc
    13141 taagaagcaa cctataggaa atccaacaat aaaaagtaaa aataactcta aaaatagtag
    13201 aaataaaagt tgtttattat tagcgcctaa tacttctaaa accccatatt gtgatattct
    13261 ctgaagtatt gaaacatgga aaatactata tactgctgct gcaccaaaca ctccaacaag
    13321 caatataatt gcatttgtat ttagttctaa attttttaag aactcaaata tattatattt
    13381 ttctatattt tttggtggtt ttactccaaa ttcaactgaa agaggccagt ttatataagt
    13441 tttttctttt gatatattga attttttcat aaatttataa ttttgctttt taatattttt
    13501 attttcatca tattttacat aaactatata agaatccttt tctttaggct gaaaataatc
    13561 gtttacaaat ccctccatag cagtttctgg gtttgaaaga cttgataaaa taccacataa
    13621 cttatagatt ttaccatcta aattgatttc tgttccaatt tctttgttta catttaaatt
    13681 gtttagagaa tatgtgtcaa gtactatctc tccatcttta ctaggaagag ttccttttaa
    13741 taattttcta cctgtcatgt ctaaatatga agaatcagca tttaaaaatt ctataataaa
    13801 aggttcatct atctgatttt taggttttgt aatgccaagt ctatctacag tgtattcctt
    13861 tgatttttta ttttctttaa ttttattaag ttgttcctta tctactttat agaaataatg
    13921 ataatctcca ctatcttccc ttatcttttc aattctactt ttgtctgcac tatgaactaa
    13981 agaaccaatt cccgtcaata atgctactga tagtactatg cttaaaacta tagatataga
    14041 ttgtttttta taatgcttta tatattgaat tgaaaatttt actagagaca tcatagtact
    14101 tcaccaccag tattcaccac aactccatct tcaatatgga taattgagtc gcatacttgt
    14161 gctatatttt cattgtgtgt taccataagt atcgtttgat ggaattttgc actactttct
    14221 tttaataaac ataccacttc cattgtagtt ttactatcga gatttcctgt tggttcatct
    14281 gctaatataa gcgcaggttt atttgccaaa gctctagcaa ttgctaccct ctgttgttga
    14341 ccaccagata gttcgcttgg aaattttaaa cgcttatccc aaattcccaa tgtcttcatc
    14401 agcatttcaa tatattcatg gtctatatgg tttcctttat ctaatgtaac aggaagagct
    14461 atattatcat aagtgtttaa tacgggcatt aaactgtagt tttgaaatat aaatcctata
    14521 tttcttcttc taaatactgt caaatctttt tttcctattt tagatatgtt ctttccttta
    14581 attattacat cacctgaagt tggattatct aatccaccta taatatttaa caatgtgctt
    14641 tttccagaac cagaagttcc aactatagca gtaaatgtat ctcgttcaat ttctatactt
    14701 actccattta aggcttttac tttagtctca ccttttccat agtgttttac taaattattc
    14761 gtcactaata tactcattta atttctcctt tcaatacaga tatatgttct ataaaatatt
    14821 gtaagtgacg ataattacac tcatcctaca aaattagttt aaactcttac aattctgtaa
    14881 gaagatacta aaacaacatc cacttcctag tttagattct actataatgt ttccattttc
    14941 tttttctaaa attaatcttg taagatatag accaatccct gtgccatttt ggttttccac
    15001 atttttactt ctgtaaaatc ttttaaaaat atcatttagt tctctactat caatacctat
    15061 tccctcatct ttaatagtaa tcttcgtata tagttccatt tttattacag aaatagtaat
    15121 cgtgctatct gatggtgagt attttattgc attttctaaa acatttacaa ttgcttctat
    15181 ggtccacttt ttattatgaa ctaattttat gtctgaaaat ggttcgagat ttatccttat
    15241 atttttagct tctgctttat taaagatagt actgatactt tgaataagag tatctttaat
    15301 tagtagttct tctacttcaa attctataac tccatcctct aatttagtca ttttaaataa
    15361 ggattgtaac agccagtcaa tcttaaaagt ttcatttttc atcttttgta aaaactcctt
    15421 cttctgtaat gtggaaatat ttatattctc cagaagttca caataaatgg ttatatttga
    15481 aagtggagtt tttaattgat gtgacatatt tgatattaaa cctttaatcg cttctttttc
    15541 tgactttgac tgttctacct ctatttctat catatcctta atttttttta cttggtgaga
    15601 aatttttgac gctcttgtat cctttgtatc tggataagta acttctttac cttccaatat
    15661 ttgatttaac atcaaattta attcatcata tcttttttga tttattttag tagatactat
    15721 aaatataaca actgtacata ttataaaggc taatataaac atattattct acctcattcc
    15781 ataaataacc cattccataa gctgttttta tatattttgg attagatggg tcatcctcta
    15841 cttttactct aagtcttctt atatttacag atacaatatt atcatcaaca taattttctt
    15901 cgctgtccca aatatggtgt aaaatttgtt cctttaataa tatttgacct ttattttcaa
    15961 ttagataact tagaagctta tattctacag agctaagttc caaacattct ttatttttat
    16021 atacttttag taacttttgg tccaatttta ttccattgga atatattact ttattattcg
    16081 atttttttct tattgctact ttgactcttg cctttaatac agataaagaa aatggttttg
    16141 taatataatc atcaagccct atttctaatc cttttacttc atcaatttct gagtctcttg
    16201 cagtaagcat tataattggt atatctgact tttcccttat tatttgacat acatcaaatc
    16261 catctccatc tggaagattg cagtctaaga ttataaaatc tatattttta ctattaaata
    16321 tatttaatcc ttcttgtttt gtataagcct taaatacatt aaatccatca ttctcaaatg
    16381 cataaaaaag accttcattt aagtttatat catcttcaat aattaataag ttcaatttta
    16441 ctctccttta gattttttag ataaataatt attttcttgc attatcatac taaaaatatt
    16501 ttaattttat ttatttcact aaaaagattg tatttaaatt tgttttcaaa ataaaaacat
    16561 cataatcaag catatagtta tatatttccc aatttaatta cgcttaaatt gaaaatataa
    16621 ctttattctt atataattaa attatacaac aataaagtta tattttttaa atatattttt
    16681 actaggggga atagaattga aaagggttga tgagagaaat actatgtttg caagagcaaa
    16741 ttacaaaaaa gatagtactg cttataatga ctattataag aaaaatcctg acaaaaaaga
    16801 aattgatgat agtataagaa atagaccaaa tttatgtagt gaaggaacaa tgacttacaa
    16861 tgagttaaac tctccaatgg caagtagtgc attcgatttt ttatctgata tcaaatcttt
    16921 atgcgaagga aaagtttctg atactaaagt agatgtagat gcaaagtcaa tgacaaaaaa
    16981 aattaagggt cttgcaaagc agtatggagc ttctgtagtt ggtataacta aattaaaaga
    17041 ctatcatatt tatacaaata ggggtagaca tgaagaaaat tacggtgaag aaattaatct
    17101 aactcataag tatgcaattg tatttggatg tgagatggat aaagaaatga taaatagagc
    17161 tccaatgata tgtgaagtta tagaaacatc aaagtgctat gtggatgcat ctatcgtcgg
    17221 catgatttta agttactata taagaaattt aggatatgat gctagaaatc atatggatgc
    17281 taattattta gtaatgcctg tttttatagc tcgtgatgct ggtttaggag atataggaag
    17341 aaatgctata cttacgaata aagattatgg ctctagactt agattaggtg ttgttactac
    17401 agatattcct cttttagaag atgaatatgt tgattttgga ctagaagatt tttgtaaggt
    17461 ctgtaaaaaa tgctctttta actgcccttc tcattcgctt tctaatgata ttaaaattgg
    17521 tgatgatgga aaatataatt gggtaattga acatgagaca tgctatatta aatggagata
    17581 tttaggtaca gattgtggta tgtgtatatc tgcttgccca tttagccaaa atctcgaaac
    17641 tataaagaat actacttcat ttaagggtaa taatgagctt attcaaaaag ctttagatga
    17701 gtatacttca aaatttggta aaagaatatt tatacctgga aatccatctt ggttgaaata
    17761 attttattta ataaagaatc tgtactagaa tttatcatag gacaggttct ttattaaata
    17821 tataaatttt taaatacttt ttctcgttta taacaattca gcaatgtttt tagtctataa
    17881 ttgacattat aaaatattaa actaaaagga gtaactgtat ggaaaaaata ttagtagtag
    17941 aagatgattc tatattaaat aaaactctat catataacct aattgaagat ggatatataa
    18001 ttacttcaaa atttacagct aaatctgcac tgaagtctat ttttgaatgt gaatttgatt
    18061 taataatttt ggatattaat ttaccagata aaagtgggtt tgaattatgt aatgaaataa
    18121 agggaaatta caatatacca ataatttttt taactgctaa tgatatggaa tgtgatatga
    18181 ttaaaggcta tgaacttgga gctttggatt atataacaaa accatttaat atcaatatat
    18241 ttaaacagaa agttaaagct tttttaaatc atcttacagt aaaaacaaaa caagattact
    18301 atagggatgg atatcttgaa attaactttt ctgagttatc tgccaatata aatggaaatc
    18361 aaattatatt tacaccatta gaatatcgta ctttaaaact gcttactgaa aatcctaaaa
    18421 gtattttaac aaggaaagta cttttagaaa aactctggga tatagatgct aactttgtag
    18481 atgaacatac tttaacatct gttataagta ggattagaag caaaattgaa aaagacaatc
    18541 ttaagtatat caaaacggta tatggtatgg gatatatgtg gttaggtgaa agaaatgaat
    18601 tttaaaaaat tttcttttag ttctatttat ttaataattg ctgttggtat aatttttaca
    18661 gctattttat ttagtatctc aatttatcta ataactgatg atttaaaact aattgctcta
    18721 ttattaatat atacaatagt actttgtgga tttatagcat taacaatatt tttacttcat
    18781 aaaaaaatag ttatattttg ttctaaaata tgtcacactt tagatagtat gatgaatagt
    18841 gaagataata ttgaaattga attagaagaa gaaactattt tatcacgtat caatcacaga
    18901 ttaattagaa tgtatgaagt tattcaagaa aatcgcaata gtattgctat agagaaagct
    18961 gatttacaag aactagtttc tgatatatct catcaaatta aaactcctat tgcaaattta
    19021 aaaatgatta atactactct actaaatcaa acatttaaaa cagaaattca aaaggatttt
    19081 ttaatggata tggaaaacca attagataaa ttagattttt taatgcaatc tatggtaaaa
    19141 acctccagac tagagacagg tataatcaca ctatctaaaa agaaaaattc aatttatgaa
    19201 acaattgcta cagcacttag tggaattttg tttgatgctg aaaaaaagaa tattgaagtt
    19261 acagttgatt gtgacccaaa tttatatata attcatgaca agaaatggac ttctgaagct
    19321 atttttaata tattagataa tgctgtaaaa tacacaaatt ctaatggtaa gatacgtgta
    19381 gctacagaat gttgggttat gtatacaaaa atagatatta ttgataatgg aaaaggaata
    19441 tcagaaagtc accaagcaga aatctttaaa agattttatc gtgaagaaga tgtccatgac
    19501 atagaaggta ttggaatagg tctctatctt tcaagaaaaa ttatatctct tcaaggtggc
    19561 tatattaaag ttacatcaga tattggaaaa gggtcgactt tttctatttt cttacctaat
    19621 aaataaattt ttaaatattt catttgtaat gattctgcaa cattttggtt ttatactgta
    19681 tttacaagat gttaaggagg tattattatg agtattttac aagtaattaa tttaaagaaa
    19741 tattatgaac aaagttcaaa cattacaaaa gctcttgatg gtgtaactct atctattgaa
    19801 aaaggcgaat ttatttctat tgtaggtaca tctggtagtg gcaaatccac attattaaat
    19861 atgatgggag gattagataa tcctacatct ggaaaggtta ttataaaggg taaggaatta
    19921 tctactatga ctgatgaaca gcttacaatc tttagacgca gaaatatagg ttttatattt
    19981 caaaattata atcttgttcc tttacttaat gtatatgaaa atattatctt accaatagga
    20041 ttggatggag agaccataga taaggatttt ataaatgaaa ttataaatat gctttctctt
    20101 gaagaaaaat tatatgatat gccaagtaat ctttctggtg gtcaacagca acgtgttgct
    20161 atagctagag cacttgtaac aaaaccagct attatactag ctgatgaacc aacaggtaac
    20221 cttgatagta gaactagttc agatgtactg agtttactta aagtgacaag taaaaaattc
    20281 aaccaaacaa ttgtaatgat tacacacaat aatgaaattg cacaactaac tgatagaatt
    20341 attcgtattg aagatggaaa aattgttaac taggaggtgt agtatatgtt tagaaataat
    20401 aatcgcacat ttataagaaa aatagcttta aatgatttaa aaattaataa acttaaaaca
    20461 tatctttcag gtatcattat aatgatttct acatgtttat tattaacagt gaccttagtt
    20521 tcatataatg cttctgtaga tttagttaat gcatctccat atcatgctat atataaatct
    20581 gtagatgaaa aagcaaaaaa tatactatat aaaaataaaa actttagtgg cgtaggaact
    20641 tacaaattaa ttggaagtaa taaaaagaca gattgtatta tgtcgattgt ttatgcagat
    20701 gatacagcaa ttaaacttat gaattttcaa ccattgaaag gcaaactacc tacaaataaa
    20761 aatgaaattg ctatttcaga aaaatattta caggaatttg gtttagataa agatattgga
    20821 gatagcatta aattaaatta ttatgatgat attacgaata aagaagttca atgtgatttt
    20881 attattgttg gatttctaga aaattattat caagataatg ccaagcaata ctatacagtc
    20941 gtttcaaatg attattttaa gaatatagct tctacacctt tacaaaataa aagtaatact
    21001 tttgacgagt caagacctga tactgtagat gtattagtta aactatcaga agaaaataca
    21061 gagaaagatt cagcttcaat taagacacag ttaaagaaaa ttggtttatc tttaggtatt
    21121 aagaaatata atatatactt aaatgataac tatattgaat caaatttaat tgatggtgag
    21181 cagattatta cagttttagt agttggaata gttgtttttt tttctagcgt ttttgttata
    21241 tatagtattt tttatatttc agttgttaat tcagttcaga tgtatgctaa gttgaaatcg
    21301 ttaggtatga cctcttttca gttaaaaaag attatttctc tacaaggaaa tattttgtca
    21361 attattttta ttccattagg ggttatagct tcttgtataa ttgcttacat aattcaacca
    21421 ttagcatggc aaatgatagc tgatttattt ataattttaa ttttatcact ggtaatgttt
    21481 ttaactgtta gaatttcttt atttaaacca gctagaatta tttctaaaat atctgctatt
    21541 gaagccatgc aatatacaga aactaaattt agaaaaaaag caaaaaattt tagttatata
    21601 aatataaaaa atctagcatt aaagaatatc gagtcaaata gaaagaaaaa tttgattgca
    21661 ttaatatcgc taagcattag tggagtttta tttatttcaa tcgctaatct tgctaattca
    21721 atagatttca aaaaacaact atctcaacaa tttgtctata acgaagatta tataattagc
    21781 attaagagtg ataacttata tgagcatatt acagaaatac aacaaaataa tccactaaca
    21841 gattctctta agaatgaaat taaatctatt catggagtaa aaaaattaat tgaatccaaa
    21901 tcaattaaaa gtaatattat agaaccttac atcaaagata gagacgaaaa acaatttatt
    21961 actctaatta aaggtattac gcctgaactt tcaaaatcac ttgaagaata tattgtcagt
    22021 ggaaaaatag actatacaca tttagattca gatagtttaa ttataaataa atatagaaca
    22081 aaatattatg gattaaattt aaaagttgga gatacagtta catttaatat ctttagtgga
    22141 aatgatatta ttaaaaagaa aatggaaatt ataggaatca ttgacaattc caatacagga
    22201 ggtatgtttt ttgcttcaga caaaacttta gaatctctaa caccatataa tacaaattta
    22261 gatttttcta ttttaataga taaaaagcat tctaaagaag ttgaagaaaa gttgttaagt
    22321 ataattcaga aaaattcaaa tttaatgctt tattcatatg aagatgatta tagaatgatt
    22381 actagagcat ttcaatatat aattatagct tcatacattt ttgttggttt aatttcttgc
    22441 tttggtatac taaatatgac caatacccta ataaacagcg ttttaattag aaaaaaagag
    22501 tttgccttac ttcaagccgt tggaatgaca agaaagcaac tgcgaaatat gctttataga
    22561 gaaggtttaa acataagtat caaagctata tgtacttcaa gtatactagg ttattttggt
    22621 tcaaatctac tttgcacttt tataaaaaat gtaattagat tagactttat taattttaaa
    22681 ttttctatat ttactatttt aattttttca ttcgtattaa ttggtataca agttttagtg
    22741 actgaattac ttgtaagaaa tatagaaaaa aaatcactta cagaacgctt acgttctcag
    22801 taagaatgta tataaaaaat agccattttt tatacaagtt ttaacataat tttttaaaag
    22861 ctaaaaggat agaaaatttt gactttacat aattttctat ccctatactt gtttttattt
    22921 tatacagata cacttcctta tatctccgag caacatcacc agagacataa acaagtatat
    22981 tccttatttt ctaaatatgt caattgcatg acttgtaacc cctactaaat cttcacgttc
    23041 acaagtagca aggtgatatt ttaaatataa tgcaaatgca tcatcatcca tagagtctac
    23101 tgcttcacgc atatataaag ccaaaccatc tgatgcgaca taatgcagtc tagttacatt
    23161 aaatgcagac atcaaatcat caatgttttc tttacgtaca agctcaaaca aatcctttgg
    23221 ttcagactta gcagcaaatg tctgtggatc aatcaaacct ttttcaatat attcagatac
    23281 attaatattt cctctatgga acccctcgtc aatgagacag ccatcagata taacatatgc
    23341 agcaaaaacc acaccaccag gttttgttac acgaattgcc tcatgtaaag cttgttgttt
    23401 atcttctttg ttataaagat gatatagtgg tcctaacagt aacgtaatat catatttatt
    23461 atctgggaaa acagataaat ccatagcatt accctgagta atgcttatat tttcatttga
    23521 ttgagtattt ttatggaaaa cctctatatt atgttcgact aattccactg cgtcaacatc
    23581 atatccctga cgtgccagtg tgtgagaata acgaccagtt gcagcgccaa tttcaagcac
    23641 acaatcacct gattttatgt acttatcaat gtaatgcata gtagtgagaa attccaccat
    23701 tccatgtttt aatgcaagtc gactatcttc atcataagta ttataaaagt caattaagta
    23761 ttgttttgtt tccataattt cacctcttct atattagtta ataaacatca aatagacaaa
    23821 ttccaatctg atttttcttg tcttataaaa gctgttaata tatttttcaa cattttttca
    23881 gcactatatc tatctaatgt taattttttt gttgctgtta agacagctat gccatgagta
    23941 taaagaaata aatctaaaaa tattactttt gcacattcca aatctattcc aatgctttcc
    24001 gaaaaaaatc ttgccttttt ttcattgtca atttctttat aaaaatcatt cgcctctttc
    24061 atcatcaaat ctaaatcatt tataaataac aacttaaata aatacgtttc ttcttgagca
    24121 aattcaatgt atgaaagcgg aagcagtaaa taagggctta cacttttaga attacgataa
    24181 ttaacaacat actgattata atattcatat gcaaaagcaa gaaattcatt ttttaattca
    24241 ttcatgtttt cataacaagt aaaaattggt cgagtggaac actgtaattt acttgcaata
    24301 ctcctcgcat ttacagcttc aaatcccact tctcttgtaa tatccaagac tgtattcaaa
    24361 atcatttctt ttgttatctt tactttaggt ggcatatatg ttcacctctc cattaaattt
    24421 ttataaaaca catgtgttat ataactatag taacatatca agaaattatg tcaagaaaaa
    24481 gctttgcatt tttttgcaat tcctaaaact tataaataaa acatgagttg caattaatct
    24541 attaatagat aacagcaact cagattttcc tttaaatact cttctttttg tctaatattt
    24601 catatacaca atcttctatt gaattaatat ctttttcttt tcttttttta gaatcaatta
    24661 aaatgaaata cagtataaaa cctacagcaa gtccccatgt tccgccttta gcagcaatta
    24721 cacctcccat aagtccaatc aatcccttct caacacctgt attacatagc tccattgata
    24781 gctttgtaca taaatatcct tgaactaaaa gtgttactga caatgctaat ggtataagag
    24841 gtgtagcaat ctgcgcaatt ggataaaaac agataaaaat tgctgaaacc catactaatg
    24901 tacccatgcc actaaaaaca gactgcatgg cttcctttcc aaccttgtac ctttgagcaa
    24961 cacttcctgt aagtgtcgca gctaatggac cgcacattgg tatatatgga gaaaagattg
    25021 ccataaaaat atttcttatc ccacttataa cattagacct atttgaattg aaatctatat
    25081 attcatcttt tctattttca ctttcagaaa gtactaagtt ttcacctgtt ataaaatcac
    25141 caaaggcaat tatgtatatc ataaaagcca ttggtatccc ttgtataaat aacattacac
    25201 ttggaaaacc tattccaaaa ggagatactg tatttatcag atttttaaaa tctactatgt
    25261 aaactaaatt gtcaaaacta aatctaggaa tttctatttc tttactaaat atacctacta
    25321 ttaaactcac taaaatagct gatactacac catattctcc taaatgaaat aaaaatttat
    25381 tcttaacttt tagagtatca aatctttcag aaaacattac aaaacaagca accaaaattc
    25441 ctactgttat agatatagga taagaatcaa atctcccttt aggattaaac tctgatatta
    25501 tagtcgatat accgacaccc attagtattc cacctttaat agaatttggt atcttactaa
    25561 ctaactttcc agctacacca gttataccca ttaaaataaa tattattcct aaatctattt
    25621 gtaaagctgt taatgcttgt attctttcaa tgcccatgct gaactttaac aaatatgcag
    25681 ttgtaagagt tatagaagct gtaatccagc caggtacaac tgggtctccc ataaaaacat
    25741 gcaaacagta taatattgag ttaataatag ccatactcca tgccacttca tagggtaatc
    25801 ccaaaatttg tgtactaagg ccaattgtgc ctaaacttgt catacttgat attatacctt
    25861 gaatagcttc tggtttctca aatctgtaat gtataaatgg aattcttatc ttaaacattc
    25921 caaattttat atgaggttgt tcccttctag cgagtctttc cataacttca ctccaccttt
    25981 caataactct tataagttta caactttata attgtttata aattatcaat gtcattaaaa
    26041 ttagtatata acatgcaaat tatataaaca ataaattaaa tctataaaat tgatagattt
    26101 tgtgcatata tagaaagacc gcttcatttt tagttactta aatcacttaa tagtagttat
    26161 ttaaatcact tgataaaggt tttttataaa attcaatagt tctattccat gattcaaact
    26221 gtccttttga atttccttct acagttccac ctgaagaaaa aatccctcca ctcatactca
    26281 tacataaagt ctcctttaga ggcataatat atggaattgg taacggatga cccatattat
    26341 tgtatgttaa taattctacg ttatatggat aattattttc ttcaaagatt tctaatgcct
    26401 ttaaacaagc ttcataacta ttccatatgt tatcttcttt tccagcaatc ataagtatat
    26461 ctgctttgga attttctatt tttattctag cctcttttct attctctgct cttttaagac
    26521 ttttcttata agtactatta aatccaaatg gaataccttt tgatatattt tttttctgtt
    26581 cttcaaagaa tatatcattg tctactttga tatatggaaa tggctctcct ttatatgacc
    26641 atgatgaagt atcatttcca ctcatcaatc cattaagagc ttggaaacaa taaatgtgag
    26701 gttgtgatac tacaaccttt ttaatttgtt catatcttga agcaagtaaa aaggccaatt
    26761 ctccaccctt tgatgtccca tgtacaaata tttctttagt actggtaatt tcattttcac
    26821 taatccactc aaatactttt tcaaaatatt ctaatggaac ctcttctaat ttatctggta
    26881 acccttctaa agcaaagtat gcaactgtta gtacattgaa tcctctagat gcaagtggag
    26941 ctgctaaaag gtctagagcg tctaaatttc catcagaacc acctagcatt aaaattgttt
    27001 tattattaga attttgttta taaaataatt ttcctataaa ctcatcagat atgtattttg
    27061 tggtaatatc ttctgattta aatattctct ttaattcaac ttcttttctt tcaatagaag
    27121 tttcaaatat catattcata attattggtt tatcaataga tatattttct gcaaaatttt
    27181 taccttcagt tttagatttt tccagcgaat aaattaaccc catacaatta tcagccttat
    27241 aagtaccttc tataggttca actaaatcta aatctacttc tcctttttca tttgaacaaa
    27301 acactccata agatgaaaat tcttctccct tacaccatgg aaattccatt ttcacactta
    27361 ttttaagttt agtatttgga attaagtctg aaaccataat cttaagtctt tcatccacaa
    27421 atgattctac attgtataca tttactttca taatctattt ctccttataa taattattta
    27481 tgttttatat aaaacagctt tatctaatac aagataaata aatttttaaa ttaagacacc
    27541 atattgacta tttcatttta tcaacatatt tcatctttgt caacatattg tcttttttag
    27601 taatttaaaa ttatggtcta tatgaaatcc agttgatttt tggctaatta aagataggaa
    27661 tgctattagg tttagaatat atatattcaa aataaaaaac cactcattta aagaatggtt
    27721 tttaatataa actatttgtt attaataagg atattttttt atttaaattt atataatcga
    27781 acatctaata tttaatgttt aataaaatta tgcatttgca aaagtatata tggcattaac
    27841 tcttgctaca tataaattct catcactaca tccaatatat ctagcatatt tttcaatctt
    27901 ttcttttgca ttttcaacat cattttcatc gctctccatt aactcattta tataaatatc
    27961 cattaatgac gctttatatt ctcttatatc atagcctaaa aattcagtca atctctcaat
    28021 ttccttacta aaatctccat cttcttttac caaaaattcg tatacatctt ccctatatgg
    28081 atttaaagtt attatttgtt ttactacttt gcattcgtca gccttcccaa taaaaccatc
    28141 tttataatta ttaaataagg cattagattt ttttatagat tcttcatcaa tataagaatg
    28201 taccgcagtt atacgatttt ctataagggc atctacaaca gcataatgaa ttttaaaaca
    28261 atcattgtat aaataattgc tcattttctt tattgtcgat tctatttcta agaagctagc
    28321 gtgaccattt tttttatttt tatttggtat aataaaagtt ttattcaaat tattaaaaag
    28381 tctcttaaaa tctaaatatt tttcacaata aacactttta aaggtatcga tatctatagt
    28441 tataattcca aaacttacta tagtctcaat tcctttctta aaagactctt ctatatattt
    28501 ttctcctaaa cttaaacctc tatcagatat ttgctttaga ttttgaaaac tatttaaaca
    28561 atctctttca aagtttttag atatttctaa agcatataat ttaaagattt tccttatctt
    28621 attataatta ttttttgtat catcaaatct gataatagct ccatttacat caaaagtaat
    28681 cattgagttt taacctcccc attagtttca tcaataaaat aaatttaata taaactttat
    28741 aatcataata ttaaacttat ttatactata attattaatt tgatgataaa agctattttc
    28801 cttctaaata aaaaatttgt ctaaagtttc taaaaaattt agacaaatct tatgttatta
    28861 ctctttaaat tatttttaaa ttattccaaa tacgccaaat aatattggta taaatatagc
    28921 tggtaacaaa ttggctactt ttatattcgt taatttaagt acatttagac caagtcctat
    28981 aattaataaa cttccagctg ctgtcatttc tgtgactaca ggatcactta aaaatccatt
    29041 taaaaatgat gctcccacac atataatacc ttcatacaaa aatacagata ttgcagaaaa
    29101 cattacacct atacctaaag aagcagtaaa tataattgaa gctattccat ctataacaga
    29161 ttttacaaat aaagtatcat gacttcctgt aagaccactt tctaatgaac caactactgc
    29221 catggctcct atacagaata ataaactaga tgatataaaa ccctctgcta tagaatcttt
    29281 tttatttcct ttagaaaatt tactttgtaa aaaagcaccc aacttattta cccatttgtc
    29341 aatatctatt aattctccta tcagagcacc tacagctacg gatattattg tcactaatgt
    29401 gttttttcct tctagtgctc ctgatatacc aatgtacaat acacataaag caattccatt
    29461 cattatagta tcgcttactc tttgaggtaa tcctcctttt attataagac ctactataca
    29521 tccacctgca attgctagac tatttgctat tactccaaac attttttctc ccctcgatta
    29581 gctaattgaa atttatccta ttattaaaac ctaaacatct atactttgta ttggatattt
    29641 taaatattcc aaaaattctt caaaaccttt tctagatata tttacagttt tagtatttat
    29701 gttaggatga aaatttaagt aatcttcttt aattacatct ttatctatgt aaagctcaac
    29761 ttttctttct gtatcattta ttaatgaaaa aggatttaca cttccaggaa gaagtttaag
    29821 tacattgtat agtctttcag gtgaagcaaa cgataatcta gaacttccta tcttatcttt
    29881 aagtgagttt aaatcaactt gtttttctcc tttaactaca ataagataat acttatttcc
    29941 tttggcattt cttaaaaata aatttttaca ttgacacccc tttgttattc catctaaaat
    30001 gactaattgc tctgctgtat atacagcttc atgctctact acatcatatt caacatttaa
    30061 tttatccaaa atatcatata ttttagtttc attcatgcta gattcatttt catcttgttc
    30121 ttttttatta tttaaatcac tcatactttt ctccttaaac acttcatcat aatatcattg
    30181 tgaggtatta ttctactata gctattgctc ttactggagc tccatcagca tcattatact
    30241 taaatggtgc tgctataaat agaaattttt ctggaacatt ttctaaattt gtaagatttt
    30301 ctatgattat ttttcctttt tcaaataaaa tatgatgcac ttcaaatact gatgtgtcat
    30361 atctatccac tgatagcata tctattccta tgccttttat attagtatta gctatatagt
    30421 ttgcagcttc ttttgttaat gtaggataac ctacatagta ctgcttttta tcccaaaatt
    30481 ttgaccaacc tgatttaaat attataaaat cactattttt tatttcatct tcatattttt
    30541 ttagatattc taattctata ttctcatttc ccttttcaag ttccaataca taagctttcc
    30601 caacaaaatt ttctatatcc aacttatcta gggtctcacc tttagtatac atatgtcttg
    30661 gtgaatccat atgagttcca ttgtgagaaa atacactaat taatgtttct tgataaccat
    30721 tttcttcaat aatagctact ttcttgatat ctggtctatt aggctctgca tatacaggca
    30781 tgtcattatg tgtcacatga gttaaatcaa aaaccttcat ctttatatac cccctatgtt
    30841 tttattttct attatatacc taaattcatt ttatttctat cagattttat tccattatat
    30901 accatactac tatatatttt ttataaacct caagggattt accgaaaaat cttggaaaga
    30961 attatcagta aatataacat cttcacccaa aaaacgttta taccttcctt cttctttctt
    31021 taaagtaatt ctctgttttt gtgaatgatt attaaaaacc attatcttaa gaggaggttt
    31081 ttttctatta gtctgcactc tttttaacat gtattttatg tgcatatcag cttcaggaaa
    31141 tgaatatcca caaaatatta aaaggtcagt atctcttaaa gttttttctg tttcattcca
    31201 aacattactt aaaaatatgt ttgacatatt cttaaaatat gttggtggta ctataatagg
    31261 ttctgttaac tcaccacact ctaaacattt tgtctctgaa ctattttcaa taagcttcat
    31321 gacaccacct tcatgtggtg taagagttac actattacaa atggggcaat aaagccaatt
    31381 caatgaccca tgtatcttat ataatttgat taatggttct tttggttttc tccaatttaa
    31441 ctcctcttta aaatttgtaa actccacacc ataatcaagc ataattggca aatcattctt
    31501 atctcttaat ccagtaactg tattgtctat atgtatatca taatttgcac taataaaagt
    31561 agtatcaaga agcaaatcat tttccaaaag attttcaact aaaagtttat gatatttatt
    31621 atctgttcta ggtgtattat gaattgcttc agccattagc agtattaaat attgtcttaa
    31681 aaaacgtata ctatcacttc tgttatttag attttctagt ccaaaatttc taaatgcttc
    31741 ttttctttgt tctgctaagt ctaaaagacc taaaacttct tcaaaggtag gaaaatttgc
    31801 attatctata ttatctttta ctatatcaat attgaacatc tgtttaaaga aaatatacaa
    31861 ttctctattc attttggtat tataatttgt gggatttagt tttttaaaat attcgctaaa
    31921 aagttcattt tgaataggta gattctctgc tgctgaagct cctgctccta aaaatattgc
    31981 tattttcata tagttcctcc taatataaat attactccta tttttatatc cattaaatat
    32041 ccatatcatt tattttatga tacaacaata aagctgtctt ttactagaaa aaatcctatt
    32101 acaagacagc cttatattat attaaatctt taataattcg taaatagaaa gagcatatat
    32161 ttgagtagct tttataatat cctctactat taagtattca ttagcttggt gttctgtttc
    32221 tggttttcct ggaaaaatag ctccaaatgc aacacaatta tctaaagctc ttgcgtatgt
    32281 tgctcctcca gatgaaagtg gtgttccatc aagaccagtt tcttcttcat atacttttct
    32341 aagtgtctta acaagaagag tatcttctgg aacatagatt gaatccaaga aatcatattc
    32401 ttcataattt aaattgtatt tatctgtcat tttctttaac tcttttacaa aatcatcctt
    32461 tttatatgtt actggaatcc ttacatctat tcctgcaaat tctttttcgt tatcaatagt
    32521 tactctacct atatttactg ttagtttacc tgatacatca tccttgcaat taggaattat
    32581 attgtttcca ttagcatctt caccaataac ttctgctaaa aacttaatta tattagaatc
    32641 tatacctata ttattaagag ctatacaaag tctagccact gcatttatac cagtatcact
    32701 tgctgctgaa tggacacttt ttccaattat acagatttta ttaccttcaa cagtatactc
    32761 aaaatttaat ttatcaagtt ctttttttaa tttatcagag tattttccaa tatagattgc
    32821 tttacctgga actgcattta aagcctttcc aactgacaac tctatatcac ttttgctatc
    32881 acaagtcaaa tgaacttgta aaagaccttt ctctgcattt gttattggga atctagaatc
    32941 tggagtaaat ccatagtttg gtatttcttc attattttct ttatatttat ttatacatct
    33001 ccaaagattt tcttcatcag ccccaaaaat gaatcttatc ctcttattaa agtctacatt
    33061 taaatccata agagctttca ctgcataaat tgctgatatt gtaggtcctt tatcatcttg
    33121 tgttcctctt ccatacaatt taccatcttc taaaacagct tcaaatggag ggtaattcca
    33181 gctttctaaa tcaccttctg gaaccacatc aacgtgacca agtataccta tcattttttc
    33241 accttgtcct atttctgcat atccataata tccatctttg tatactgtct taaatcctaa
    33301 agatttacat aagtctaaga tacctcttaa agctttatct acattttcac caaatggaca
    33361 attttcagtt gcttcagata taacactagg tatttttaca ctttcttgta ttgaagaaat
    33421 catttcatct tgtagagaat ttactttttc tttaatttgc tctttcatat tatcaccctt
    33481 caattttcat cataatatac taataaatat tttgaccttt aaaaaaaaaa atatgcttat
    33541 tttttcagtt tttttataaa ttcaatcgct tttaaagtcc ccatttttga atggtcaaat
    33601 gtaagtccac cttgaaaata aacattataa ggaggtctta ttggagcatc tgcacttaat
    33661 tctatagaag aaccttgtat aaatgctcct gctgccatta tgacttcact ttcgtatcca
    33721 ggcatgtccc aaggaacagg ttttacataa gaatcgactg gtgcagcttc ttgtatacct
    33781 tcacaaaaac ttattacttc atcagcatta ttaagtctaa tacattgaat aatatcactt
    33841 cttaagtcat catattttgg taatacatca tatcctaatt tttcaaaagc tcttgagcaa
    33901 aagatagctc ccattacagc ctgagatact atataaggcg ccaagaaaaa accctgaagt
    33961 acatttctag ttgttccaaa agtaagacca cattcttttc ctattccagg agaagtcatt
    34021 ctataagata taagctcaat caaatctttt ctaccagcta tatatccacc tgtcaatgca
    34081 agcccaccac caggattttt tattaaagag ccagccataa catctgctcc aacatcagta
    34141 ggctctttgg tatctaaaaa ctcaccataa caattatcaa ccattactat agcttctggt
    34201 ttaacagact ttattacttc tatagcttct tttatatcac taattgaaag tgattttctc
    34261 caagaatatc cttttgacct ttgtatcata acgagtttag ttctatcatt tattttgttt
    34321 ttaattcctt ctaaatctaa gtttccatcc tctaaaaaat ctacatcatc atatgtaaca
    34381 ccatactctt tcaatgaacc tttttcttct cttatgccta taactccctc taaagtatca
    34441 tatggtctac cagtaacaga taaaatttca tctcctggtc taactatacc ttgtatacat
    34501 aaagtaagtg catgtgtacc atttacaatt attggtctta caagagcatc ttctgtatta
    34561 aatactttag aataaatttc ctctattttt tcacgaccaa tatcattata tccatatcct
    34621 gtagtccagt taaagtgcat atcacttagt ttagattctt gcatagcttt taaaacttta
    34681 tattgattat attctcttat ttcttttatt tcttcaaact tatcttttat ttcttccatt
    34741 atttcttgcg atagtttaaa agtatcatca tctattccat agtagtcttt taataattct
    34801 tttgtctcat taagcatttt atcacctgtc tctttattat tttatttaaa catgtaaagg
    34861 agactatttt actaaaacaa ataaattata gtttataagt tagtataagt ctcctctcaa
    34921 tcatcttaaa ttaaattaat tatctatctg ttgttattat tattgttgtt ttggttttga
    34981 tttgttagat taatatactt tccaggttgt atagttgata cagcatgttt ataaatcatg
    35041 ttttgttgtc tattatcccc ttctattaat attatataac tatcaaaccc ctttacaagc
    35101 cctttaactt gcactccatt aactaaatat atagtaacag gtatcctttc tttccttgca
    35161 ttatttaaaa acaaatcttg taaatttaaa actgtatttt tcattagatt tgtaccctcc
    35221 caagattaaa ttgaacttct aaaatattcc taccttatat tctatataag ttatgacaaa
    35281 ttttcaatat taagttgtat acttaaaaat ttatatatta ttttatagaa tctttaatat
    35341 ataagattat ttcatttttt aattcatcta tattttcata ttggtctaaa tcaaaccatt
    35401 ttgctgtttt atatctttta aaccatgtta tctgacgttt tgcatacctt ctagaatctc
    35461 ttttaattat ttcaatagct tcttcgtatg tatattctcc atccagatac tttaatatct
    35521 ctttataacc tataccttgc attgatatca tatctttttt aaatcccata cttaaaagtt
    35581 tttttacctc ttcaaccaat ccatttttaa tcataatatc aactctcata tttattcttt
    35641 gatacaaatg ttctctatct ctatttagaa ctattattat tggttgatat tcttcattaa
    35701 attttaaatc acttgaaaaa tcattcattt tcttaccact tagacaaact tcaagagctc
    35761 ttatgaccct tttagtattg tttttatgga ttctattagc agattcttca tcaagttctt
    35821 ttaacttatt atgcatgtaa tctattccct tttcagcaag ttgtttttga agttcttctc
    35881 ttaattcatt attggcatca gattgtgcaa aatccatatt atatattaaa gagtttaaat
    35941 aaagtcctgt gcctccagtt actaaaacac atttttcctt tttatttatt tcatgtatat
    36001 atttttttgc tctttgttga aactctgaaa ctgaaaatgg atagtcaggc ttaacttcat
    36061 ctataaggta atgcttcact ccttgcatct ctttctctgt aacttttgca cttccaatat
    36121 ccatatattc atatatttgc atagaatcag ctgaaattat ctctgcattc atatcttttg
    36181 ctaattttat tgacaaatct gtttttccaa cagcagttgg acctgtaagt attataagtg
    36241 gtattttttt cataacattc ctttccatat attttaaata tccaaatatt ttacataatt
    36301 cttttaaaca ttttttctat ttctgtctta gatatttcga ccattatagg tcttccatga
    36361 ggacatgtaa atggattctc acacttttct agctgttcca ataaactttt tatttcaata
    36421 tcgtatattt tatcatttgc ttttatagca gacctacatg ccatagatgc aaatctctct
    36481 ccttttaaat cataattact tgtaatttct tctatattat caattatttg aagtataaat
    36541 ttctcagttt caggtactcc aaatatagta ggaacacatc ttaccattat atgattattg
    36601 ccaaaaattt ctagctcaaa accaaatttc ataaataact ccaagttatt ttcaatttga
    36661 agcatatcta catttgatac ttcaataacc actgggtcta aaagtatttg catatttata
    36721 tcttgtctat agaacttctc catatatctt tcatatagta ttctttcatg tgcagcatgt
    36781 tggtccaaca aatacataga atcatctttg ctcaaaatta tataagtatt aaatacaact
    36841 ccaattacag aatatccgta taaagaaaac ttgcgttttg aactacacaa gtattcttct
    36901 tcactgtcct taatactatc tcctaagtaa taatttcctt catttttaat tccatcaact
    36961 tgaaactctt cttgtatatt atcatttgca ctatcttcac ttagataatt aatgtttttt
    37021 acatcatctt ctacacttgc atttaggact tcacttactg attgaaattc atttattggt
    37081 ttttcacttg ataaagtaac aacttcaatg acttcatcct tacctttagt aatattacta
    37141 tttttaggag tagattctaa taaatcatta tttcttaatt tataatctgt acttttttct
    37201 ctactgttaa tagcgattct aggttgtgtt tttttatcac tataagtagc atattttcca
    37261 ataagatttg agtgtattaa tttaaccttt aaaaaatctc taagttctat atacacttct
    37321 tgttcttttt caaatttaat ttcaagttta ttggggtgta tatttacatc tatacatgat
    37381 gggtctactt ctatatttaa gaagcacact gcatgtttac ctattggaat tatagactta
    37441 taactttctg ttattgcatc tatgattatt ttacttttta caaatctttt atttatatag
    37501 atatgttgta agtttttatt tgaacggtat atattgttat tacctatata tccattcatt
    37561 ttaaaatgat tgcacttaaa ctctacatct attatatttt ccgttatttc ttttccatat
    37621 atacttctaa tagtgtttac aagttttcca tcccctggag tatttagcat ctgtttattg
    37681 ttatttgtat atttaaattg aatgtttgga ttacctattg caagtttatt tatcaaatca
    37741 cttatattta tagtttctgc atgagtagat tttaaaaatt tctgtcttgc tggtgtatta
    37801 aagaaaatat cttttataat gatggtagtt ccattagtag aacctattgg ttctttcgat
    37861 attatttttc caccttcaac atatatttta gttcctatta tttcatcttt agttttagtc
    37921 gtcatttcta attttgatac agctgaaata gatgccaatg cttctcctct aaaaccaaga
    37981 gagtataaat catacaaatc atcaattttt ttaattttac tagtagcatg tctcaaaaat
    38041 gatttttcaa cttcacttga aggtattcct attccattat ctgtaatctt aattagactt
    38101 tttcctccat caattatatc tatggaaatt ttatttgcac ctgcatcaat ggagttttca
    38161 ataagttctt ttactacaga agaaggtctt tctacaactt cacctgctgc tatcttattt
    38221 atagtcaaat catctaggat gttaattata tttttcattt ttacacctct aagattttat
    38281 ttcttttgct tttctttgaa gattataaag agagtttata gcatctaatg gagtcatatt
    38341 taaaatatcc agatttaaaa tttcttctga tagtatatct ctatttacac tatcaaaaga
    38401 gatttgagtt aaggctacct cttttacaga atcttcttga ttaattttct ctttatcatc
    38461 attttgcttc attaaagcaa cttcatcatt taatgcttta aatttagaat ttaatttatc
    38521 ataatccttt tttatatgtt tgtagtcttt tactataagg tcatgttcta tttttagaga
    38581 ttcatattta ttcttaaaat tactttgatt taccttaact aattcttcgt ccaaattact
    38641 attaattata ttattgtttt ctttatctcc atttattgca acactgttgt aaacatgatt
    38701 tttttccaag tctttaagta tatattttgc tctttctatg acttcgtctg gtaatttagc
    38761 taatttagca acatatattc cataactctt gtctgctccc tgaggaatta tttttcttaa
    38821 aaatattatt ccttccccat cttctttgac tgctattgaa taatttttaa cctctttaaa
    38881 ctcttcttct aaatcagtaa gttcatgata atgtgttgca aataatgtct tacatctaat
    38941 atttttttga atatattcca ctattgacca tgctaaactt atcccatcat aggtactagt
    39001 tccacgacct atttcatcta atataactaa acttctctct gttgcatttt ttaatataag
    39061 ggatacttcg ttcatctcta ccataaaagt agattgacct tgagataaat cgtcactagc
    39121 tccaactctt gtaaatattc tatctaatat tggaatatca gcagactctg ctggtacaaa
    39181 tgagcctata tgtgccataa gagctattat agcagtttgt ctcatatatg ttgatttacc
    39241 tgacatatta ggaccagtta ttatgttaat aatgttttct ccacgattta aataagtatc
    39301 atttggaacg aaattttcct ctcctactat attttcgaca acaggatgtc taccatttct
    39361 tatatcaagt ttattatttt catttattgc tggttttacg tagttattta tatgtgcaac
    39421 tgttgcaagg gacacaaaaa catctatatt agctatagtc ttagccactt tttgtattct
    39481 atctatgttt ttatatattg tatctcttat ctctacaaat atctcgtatt ctaaagattt
    39541 aatcttttcc tctgcatgta gtatcttttc ttctatttcc tttaattctg gagttatata
    39601 tctttctgca ttactaagtg tttgctttct tatatatgtc tcatctagct ttgcctgttt
    39661 aaagttagct tttgttattt ctatataata cccaaaaact ttattaaatc ctatttttaa
    39721 tgatttaact cctgtttttt ctctttccct gttttctatt tcctttacaa gaaaggctcc
    39781 attcttagaa atctctctta gttccttaag ttcatcacta aaatctgatt taattatgtt
    39841 tccatctttt atggttatcg taggttcttc tagaatagct tcatctatta aattgtatat
    39901 atcatctagt ttatccattt ctgaaacata ctcttttagg atttttgcat ttgataaatt
    39961 tatagtatct tttaaattag gtaacttttc tatcgaattt tttaagtgaa ttaactcttt
    40021 tggtgttact ctctcaaatg ctatctttcc acatattctt tctatatcgt atatattttt
    40081 aagtatatca tttaggtctt ctcttaatat aaagtcatct tttatttctt ctattacatc
    40141 taatctattt tcaattttag atttatttat caaaggttct tctacatatt ttcttaaaag
    40201 tctacctccc attgctgtag aagttttgtc aagtacatgt aataatgaac cttttttctt
    40261 attccctctt atagtttgag taagttctaa attagttcta gtaaacatat ctaaaaccat
    40321 atattctgat gaattgtata tatttatatt attaatgtta gatgttattt gtttttgagt
    40381 attgtatata taattcagaa gaattgataa gcttgacttt attagacctt tatcatcaaa
    40441 ctttagtttt tgtagataaa catctggaaa atattctttt aatatattta tatccaaata
    40501 gttatcacta aaactttcat ttatgtatat attacttaca gtagctatat ctctaagctt
    40561 ttctataaag tctaaatcat ttataattat ttcagttgga tggatttttg caatttcttc
    40621 aattacctta tcttcattta aacatgttgc attagtttct cctgtactta tatcaacata
    40681 agttagacct atattagtac catccttata caaagataat aaataattat tctttttatt
    40741 ttcaagtaaa tttccatcta aaactgtacc aggagtgatg actcttataa cctctcttct
    40801 aacaattcct tttgctgtag atgggtcttc catttgttca cctattgcta ctttatatcc
    40861 attttcaact aattttgata tatatgaatt ggctgagtgg aaaggaacac cacacatagg
    40921 agcacgttct tctaaaccac aagcttttcc tgtcaatgct atttcaagag cttttgaagc
    40981 tactaatgca tcttcaaaaa acatttcata aaaatctcca agtctaaaaa acagtataca
    41041 gtctttatat ctatttttta cttcaagata ctgtttcatc ataggagtta atttatttat
    41101 atctatttgc attatcaaac ctccttcttt tctaattaaa tattatatca tagtagatga
    41161 ttattattaa cacaatatat aaaattttga catataacta aaaacaatcc aaaatgtata
    41221 ctcacatttt ggattgctaa gtttaatgct tattaaataa aaatattttt ttttacaata
    41281 aagatattat ttctttttta agtttaatga tattttcaag gttatcttca tttaactttc
    41341 tactatctat aacaaattct ttctttacta ttgatggttt ttttgaaata acataaattc
    41401 tgtttgatag cattatagct tcgtcaatat catgagttat aagaagtatt gttgaattta
    41461 cttttttctt aacatctaaa agccattttt gcatggatgt tttagttaat gaatcaagtg
    41521 ccccaaaagg ctcatctaaa agcattatgt cattagaatt tataaaagtt ctcaagaagt
    41581 tagctctctg tctcattcct ccagaaagtt ctgaaggata tttatcttca tatccgctta
    41641 atccaaatac ctcaaaataa gattttacca ttgacctagc tttactcttt ttttcttttt
    41701 ttaaaattag tggtagtgac acattatcta ttatagtttt atatggcaag agtaaatctt
    41761 tttgatacat ataactatac ttaccattta tattcacttt gccactatca taatctgtaa
    41821 gtttagttat aatattaaaa atagtacttt ttccacatcc acttggccct aatatagaaa
    41881 caaactcacc ttcataaaca tcaatactta tatcttttaa aacttcaata tctttataag
    41941 tttttgatac attttctata gatactttta cttttttatt gtggtaaaaa ttcatttgtg
    42001 taagcatcac ttgctttcat atccttatct attaatttat attcttttaa gaatgatgta
    42061 tagttgttcc atacactatc tttcatttct ccccatcgtg gtgcatcatc tttgtacttg
    42121 cttgctaaat attcttgact ctttaaagct agtttttcat ctacttcagg tgcatgtttt
    42181 actaaaattt ttgctgattc ttctggattt tttattgcat attcataacc ttttgttgta
    42241 gcttttaaga attttttagc aagctctgga ttatctttaa gcacagtttc attagaaatt
    42301 ataagtggtg tataataatc caatctctca tctaaatctt ttactggtat aaagtttaag
    42361 tcatatccaa ttaaatcagc tttaactgca tcccaacctt caaatatcca tgcaaaatct
    42421 acagttttca tagctgcaaa gaaatcatct tgaccagtat taactatctt taacttatta
    42481 aaatcagctc catttttctt cataactgct ttaaaaacag cttcttctga tggagagccc
    42541 caaccaccat aagtttttcc ttcaaaatct ttagctgttg ttatattctt ttcttttggt
    42601 gatgcaaacc cagaagtatt atgttgtata acagttgcta cagcttttat tggaagtgga
    42661 tcttcactag tctttgcata agtaacttgt tcttgatagc ttataccaaa gtcagctttt
    42721 cctgttgcta ctaaagtttc tgctccactt tcaggtggtt gtactatttc aacatccaaa
    42781 ccttcttctt tatagtaacc cttatcaagt gctacaaata atccagtatg atttgtattt
    42841 ggtgtccaat caagcaccat agttactttt ttagaagaag tatctttatc attgtttttg
    42901 tctttattgt tatttgaaga acatccagtt attgttgcca atgtaaaaac acttactaaa
    42961 cctaatattc ctaacttttt aaaattcata attgcctccc tcaaatacct ttaatctatt
    43021 aatctatgtc ttataacaac tttttttaca gcctgtacaa ttcctacaaa cattaaacta
    43081 aatataacta ctaatatagt agatgcaaat actttatcaa gtgcatatgc actttttgac
    43141 ctaagcatat aaacaccaag tcctttcgtt ccacctagcc attctgcaac agttgctgcc
    43201 attacagcat atgttgcaga tattttaagt ccagaaaaaa atttatccat tgccatagga
    43261 aatttaaggt gaatgaatgt attaacttta tttgagtcca tagtcttaaa taaatttaaa
    43321 taatctttat caatattctc aatgccatct acaaaactta ttaatatagg aaaaaaacaa
    43381 gtaagcatta ccatcaatac ttttggcatt gttccaaatc caaaccatat cataaagagt
    43441 ggtgctataa ttattgtagg tatcatctgt gtaaccaaca taataggata aagacatttt
    43501 tttattattg atacaaaatc catcaatatt ccaattacca atgatattat aattgcgact
    43561 ataaaaccaa gtattgcttc tcctatagta accacagcat gcatggataa attttgataa
    43621 tctttaaaaa acacattaat tatatctact ggtgtgggta gtatatactg aggaacttct
    43681 aaaactccta ccatagattg ccaaagaata agtataaaca aaaatgttgc tattggatat
    43741 atactatctt tgactttatc tatgatttct aagtttttca tctatagtca gacctccagc
    43801 tttttcatct actctagcta ctgttatgac tctttcacat ccaagattaa aagggatttt
    43861 atgtaccttt tttaataatt caaaaacttc atcaaaatca ccttcaacag tagtgctcat
    43921 tgctcctatt tcacatttaa gaccactttt ttcaatttca gcaatacaag catctactac
    43981 tttatacatc tcttcttcac ctttgtaagg tcttagtggc attactgcta tgtctgcgat
    44041 taccatttaa atcactcctt taaaaataat taatacctac taggtatatt ttgtttttta
    44101 ttaaaaaatt ttattactaa aaagatatta tatttaaata aaagaaaagt cttatccaaa
    44161 ataggataag acttattttt atcttaactg gtctccctac actggcatta tccaaacagg
    44221 ttcaaagggt cgagataaaa tctcctctca gcacattagc tccccgaaat atattatatc
    44281 ttttctactt gattatacta cacttatcat attttaacaa attttttatt tatattagat
    44341 attttatccg ttcaaaaata tctaatataa attgtacatt attacaccaa ataaattatc
    44401 ttaaaatggt aatttatcaa catcttccaa aactagatta actgaatttt ctagttttta
    44461 ttttgctatt tcaagcatat ttggatgtat atcaattcca tacaaatatt gttctaaatt
    44521 tctgttttca ctaatctaat ttgcaatcgt tacagattac agattagaaa tagcttttag
    44581 actctactta tctttatcta caaaaaatcc ttcaataaat tcagtaataa tattcagtaa
    44641 tctaaaaata aacctctatc tggattttta agatacaagc cttttaggaa gtatcttttg
    44701 tgataatata aaaattactc gtcattatca tcaaaatcct cagcaaccaa cattttagat
    44761 aagaatcaaa ctctataata taattatttt atccaattta tttttgagaa ataaaatatt
    44821 tttttaattt ttaaattttt taatatgcac cttgaaaaaa gaaacgtttt cctatattat
    44881 ggtaatagta gaataaaact aaataggcaa aactagagaa atttagtgac gcaaagctat
    44941 agggactaag acttatgaaa tgagttatgt cagccagttg ccaaagagat attttctttt
    45001 tgtttttatg aatgtttttt agggggatat atgatatatg tttatggata aaatggatag
    45061 atgtacacat atactaactg catatatatg cagttcacgc gattactgca attttataga
    45121 tacacaatta aatgatttta tactagaata tggagagaat gtggtagaat cttgtttgca
    45181 tcaagtgatg gtattggtaa gcagatataa ttaaagaaat tttattttct agaaaaccct
    45241 ttttagtttt atctacgttt tataattcat taaagaacta attaaaataa gcttcattat
    45301 atatttttca taaagtaaac agttgagttt atctcatata ctgtttaccc caattacata
    45361 attctaataa tatagattct aatgatttac cacgtttagt agacaaatat tcaacttttg
    45421 gaggaatagt cggataagaa atcctcttaa taagtccatc tctttcaaga tactttaatt
    45481 cttgtgacag tatcttatga cttatatgaa caacttcttt ttttaattga ctgtaacgta
    45541 ttggctcttg ttttaaaata tgccataaaa taattgccct ccatttactt cctatcacag
    45601 tcatggtaaa accaattgaa caattaaaat ctttttgtct ttcttcaata tttggaaatt
    45661 cttcaaaatt attatctata tccatctcaa tcctctcttt caaaatagta agtatctaac
    45721 taaaaagtgc attcttgttt agcgtactaa ttcgtaatat aatcttgatt gtagtataga
    45781 gccttacata atactacttt tttagatgta tatgtttaaa ttttaatata gacatatgtt
    45841 attttattaa ttttatttaa acagataata ataaataata atttatctca agttttctat
    45901 gtagataaac taatctaatg tttagaaagg attatataat atgaataaaa aatttcgtct
    45961 ttatcaagtt gactcattta caaaggaaaa atttacaggt aatccagctg gtgtaattac
    46021 aaatgctgat ggcttatctg aatcacaaat gcaaaaaata gcaagagaat taaataattc
    46081 agaaactgct tttattttca ataacttggc taaaaatcat caagaaaatt atgatgtaca
    46141 tatcagattt tttacaccaa ctaatgaggt tcctatatgt ggtcatgcta ctattgctgc
    46201 ccattatgtt agagcagtag aaaaccaatt caataacaaa acagtatttc aaaaaactgg
    46261 tgctggaata ttaccagttg atattataaa aaaagataat gattatgaaa taattatgac
    46321 acaaggaaaa attgaagtat ctccaccatt acaacaaacc tatcaaaaaa gattacttaa
    46381 tgcatttggt attttaaagg aagatattcg tacagattgt cctattgcca tatcatctac
    46441 aggacattca aaagttatga ttggaataaa ggaattatca acattaaata gtttatctcc
    46501 aaatttatat gaattatcga aaataagtca agaaataaaa tgtaatggat attatatttt
    46561 tacattacat aataaagaaa ctctacaagt tcatggaaga atgtttgctc ctgcaattgg
    46621 tatttcagaa gaccctgtta caggaaatgc taatggaccc ttaggtgcgt accttgtaca
    46681 ttataaagta atacctacac ccaacacaga gtttatattt gaagcagttc aaggagaagc
    46741 aataaaacgc aaaggcaaga tgttagtaaa agtaattatt ttagataata agccagttga
    46801 agtaaaaata attggaaaag ctgtaattgc attctcaaca aacatagaaa tctaattata
    46861 gattccttct aagtaaattt ttgaactttt taatactaat ttaataacaa actaatttat
    46921 atacaatcaa attaattttt ttattatttg ttaatatata tttat
//
LOCUS       DACXMZ010000005        45576 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.005, whole genome shotgun sequence.
ACCESSION   DACXMZ010000005 DACXMZ010000000
VERSION     DACXMZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45576
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.005"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            193..852
                     /locus_tag="JD016_01110"
     CDS             193..852
                     /locus_tag="JD016_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4998225.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            1063..2121
                     /locus_tag="JD016_01115"
     CDS             1063..2121
                     /locus_tag="JD016_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAU4998226.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKRFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            2372..4516
                     /locus_tag="JD016_01120"
     CDS             2372..4516
                     /locus_tag="JD016_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2786..2788,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HAU4998227.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            4640..5398
                     /gene="fdhD"
                     /locus_tag="JD016_01125"
     CDS             4640..5398
                     /gene="fdhD"
                     /locus_tag="JD016_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAU4998228.1"
                     /translation="MDYKYLNSFNVEVSKINEHEVSIDNEEIIAEYPLSFILNDKYVN
                     TFLCTPYNLEELIVGFLKTKGYIENKKSLLKFEINESERFAKVTIQEKQKNQDEQIIF
                     LNSLDYITCAPVKSNIKINYDTIYSIMDKNLNSSELFKNTGGVHSVSVFNFDKKIVTC
                     EDVARHNAMDKAIGFCVLNDITLKDKIVAVSGRISLEMILKAAMTQIPIVVSKSAPTN
                     LSIELSKKLNITLIGFVRGRRMNIYANPQRVIKK"
     gene            5409..5582
                     /locus_tag="JD016_01130"
     CDS             5409..5582
                     /locus_tag="JD016_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998229.1"
                     /translation="MCTKKEKQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            5640..6191
                     /locus_tag="JD016_01135"
     CDS             5640..6191
                     /locus_tag="JD016_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAU4998230.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            6207..7592
                     /locus_tag="JD016_01140"
     CDS             6207..7592
                     /locus_tag="JD016_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAU4998231.1"
                     /translation="MTFMIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCV
                     MCGQCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSD
                     PNVFTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIME
                     EGSELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYG
                     AEINNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGID
                     FKNLREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGEN
                     GFRKSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQP
                     KILLEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLH
                     TTYCIGKEVAK"
     gene            7592..8107
                     /locus_tag="JD016_01145"
     CDS             7592..8107
                     /locus_tag="JD016_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAU4998232.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(8185..9468)
                     /locus_tag="JD016_01150"
     CDS             complement(8185..9468)
                     /locus_tag="JD016_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAU4998233.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            9656..10078
                     /locus_tag="JD016_01155"
     CDS             9656..10078
                     /locus_tag="JD016_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HAU4998234.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISSECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            10180..11118
                     /locus_tag="JD016_01160"
     CDS             10180..11118
                     /locus_tag="JD016_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAU4998235.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            11304..13328
                     /gene="ligA"
                     /locus_tag="JD016_01165"
     CDS             11304..13328
                     /gene="ligA"
                     /locus_tag="JD016_01165"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAU4998236.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     INFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKEMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            13488..14837
                     /gene="rph"
                     /locus_tag="JD016_01170"
     CDS             13488..14837
                     /gene="rph"
                     /locus_tag="JD016_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAU4998237.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            14837..15307
                     /locus_tag="JD016_01175"
     CDS             14837..15307
                     /locus_tag="JD016_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAU4998238.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            15501..16787
                     /locus_tag="JD016_01180"
     CDS             15501..16787
                     /locus_tag="JD016_01180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAU4998239.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            16937..17521
                     /gene="clpP"
                     /locus_tag="JD016_01185"
     CDS             16937..17521
                     /gene="clpP"
                     /locus_tag="JD016_01185"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAU4998240.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            17540..18790
                     /gene="clpX"
                     /locus_tag="JD016_01190"
     CDS             17540..18790
                     /gene="clpX"
                     /locus_tag="JD016_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAU4998241.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQSADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(18861..19388)
                     /locus_tag="JD016_01195"
     CDS             complement(18861..19388)
                     /locus_tag="JD016_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HAU4998242.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(19376..19888)
                     /locus_tag="JD016_01200"
     CDS             complement(19376..19888)
                     /locus_tag="JD016_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HAU4998243.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            20068..22431
                     /gene="lon"
                     /locus_tag="JD016_01205"
     CDS             20068..22431
                     /gene="lon"
                     /locus_tag="JD016_01205"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAU4998244.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKESFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            22421..23032
                     /locus_tag="JD016_01210"
     CDS             22421..23032
                     /locus_tag="JD016_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAU4998245.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(23297..24667)
                     /locus_tag="JD016_01215"
     CDS             complement(23297..24667)
                     /locus_tag="JD016_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAU4998246.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(24842..24957)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25245..26324
                     /locus_tag="JD016_01220"
     CDS             25245..26324
                     /locus_tag="JD016_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAU4998247.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            26716..27396
                     /locus_tag="JD016_01225"
     CDS             26716..27396
                     /locus_tag="JD016_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HAU4998248.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            27419..28768
                     /locus_tag="JD016_01230"
     CDS             27419..28768
                     /locus_tag="JD016_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HAU4998249.1"
                     /translation="MPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDIYALQDLRLAT
                     GKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENASDIHIEPFKE
                     HLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGRVDIKLGGNLI
                     DIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINKRAGILLVTGP
                     TGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGLSFDVGLRAIL
                     RQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLLEMQIPPYLLN
                     ASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKGYFGRTAIYEI
                     LEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLEECLVVNEMI"
     gene            28939..30024
                     /locus_tag="JD016_01235"
     CDS             28939..30024
                     /locus_tag="JD016_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HAU4998250.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMRISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            30078..30437
                     /locus_tag="JD016_01240"
     CDS             30078..30437
                     /locus_tag="JD016_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAU4998251.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            30490..31362
                     /locus_tag="JD016_01245"
     CDS             30490..31362
                     /locus_tag="JD016_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HAU4998252.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKGHKKVFIDKSIIKEINFDIK
                     NDSNSIEKVKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            31363..31806
                     /locus_tag="JD016_01250"
     CDS             31363..31806
                     /locus_tag="JD016_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998253.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRYGEYYRFRIKS"
     gene            31821..32405
                     /locus_tag="JD016_01255"
     CDS             31821..32405
                     /locus_tag="JD016_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998254.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            32646..33695
                     /locus_tag="JD016_01260"
     CDS             32646..33695
                     /locus_tag="JD016_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HAU4998255.1"
                     /translation="MEVILLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQ
                     YTDLYSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKK
                     VMTVGFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTED
                     VKLLRSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKD
                     MKSFLIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELVEEECKVLDTLKE
                     NVEKENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDI
                     SSSTLGKRYKDLKIS"
     gene            33776..34141
                     /locus_tag="JD016_01265"
     CDS             33776..34141
                     /locus_tag="JD016_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998256.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            34146..34463
                     /locus_tag="JD016_01270"
     CDS             34146..34463
                     /locus_tag="JD016_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998257.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            34511..36115
                     /locus_tag="JD016_01275"
     CDS             34511..36115
                     /locus_tag="JD016_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998258.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            36239..37867
                     /locus_tag="JD016_01280"
     CDS             36239..37867
                     /locus_tag="JD016_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAU4998259.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            38132..39481
                     /locus_tag="JD016_01285"
     CDS             38132..39481
                     /locus_tag="JD016_01285"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAU4998260.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            39637..40716
                     /locus_tag="JD016_01290"
     CDS             39637..40716
                     /locus_tag="JD016_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAU4998261.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            41061..41969
                     /locus_tag="JD016_01295"
     CDS             41061..41969
                     /locus_tag="JD016_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAU4998262.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKILFEASNFNTKIGG"
     gene            41974..44343
                     /locus_tag="JD016_01300"
     CDS             41974..44343
                     /locus_tag="JD016_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAU4998263.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKQDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFKSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRILQDEIIGRTEQTF"
     gene            44668..45471
                     /gene="proC"
                     /locus_tag="JD016_01305"
     CDS             44668..45471
                     /gene="proC"
                     /locus_tag="JD016_01305"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAU4998264.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT        18696 a         4818 c         8259 g        13803 t
ORIGIN      
        1 aagtaaatac gtgtaaaatg aatataaagt ttttgaaata taaagttttt gtatctaaaa
       61 tattttattt gtaaaatagt aattaattat tgtaagctat aatatgagag tattatggtt
      121 aatgtgaatt ttaaagatat tattaaatat ataaattaga atgctattag atggataaat
      181 ggaggagtta ttatgtctaa gatattagta gtagatgatg agttagatat gttaaagttg
      241 attgaaaatg tgttgaaaag agatggacat gaagtaaagg taattagtga tgtagaaaag
      301 gctatgaaca tggattttaa ttattttaat ctaataatat tagatgttat gatgcctaag
      361 atggatggtt ttgaagtatg taaaactata agaaataagg ttgattgtcc aatactgttt
      421 ttgacagcaa aaaatatgga aagtgatatt atgtacggtc ttggtatagg tgcagatgac
      481 tatataacca aaccttttgg tgtaggagaa ttaagggcta ggataactgc tcatttaagg
      541 cgagaaagta gagaaaaaaa gaatttatta actatctcaa atgttcaatt taacttttta
      601 ggcaaagaag tttcaattga tggtaaaaaa attaatttta ctaaaagtga atatctaata
      661 tgtgaatttc tagcaaaaaa taagggacaa gtattttcaa aagaaagaat atatgagtcg
      721 gtatatggtt ttgatggaga aagtgatata agtactatca cagagcatat aaaaaatata
      781 cgaagcaaat taaaacaata tgatatttat acaatagaaa ctgtatgggg gatagggtat
      841 aaatgggtgt aataagtata agtgttgttt agtgaaaata ttatatatat ctacaaaatg
      901 tttgtaaaat attaatctat atattaaaat ttaatttata gattaatatt ttttgtaaat
      961 gacaaaattg taaggtattt tgttataaaa tattaagatt catagtgtat actaataata
     1021 tagtatttag tataaatctt gagcaaagga tggttatttc taatggatag actaaagaat
     1081 attagaaaga gtttactaca gtatacattt ttatttttgt taataggtat aacaatttac
     1141 ctagtgttta aaacattgga tattaaaatg ctatcaaatg ttatggttat ggtagataag
     1201 aggtttttat tcataggtgc tttggcaata atgtgtcaca taatgcttga aggagtagta
     1261 atgaagataa ttatagaaag cactcacaaa gttaatatta gatttattgg ttttaagcta
     1321 gccattatgg gattttatta taatttaata actccttttg cttcaggaag ccaaccagta
     1381 cagatatatg tacttaaaaa atgtaaaatg ccacttagta aggcaagtgc agttgttacc
     1441 aataagtcta ttatcttcca aatagtagtt actttttatt gtacaatact tgttttaatg
     1501 aattttgcta tgttagaaaa acaaatgaaa atgattatgc ctctagtgtt tctagggata
     1561 gccattaatt catttgtctt aattatgata atattagtta ttttaaatcc taaaaaaata
     1621 aaatatttta tgagaattac tataaagcat ttatcaaaaa ttaagttttt aaaattttta
     1681 gataataaga ttgaatccat agaaagtttt atagatgatt atagtaaagc aataaatttt
     1741 tttgcaaaga ataaaaaggt attaatttca actataatag taacttttat tcaattaagt
     1801 gcttacttta gtgtttcatt ttggatatat aaagcattta atttacaagg ttatagctat
     1861 atttatatac tgacattaca agcattttta tatatggcaa tatctccaat accaacacct
     1921 gggaatgtag gagctaatga attagccttt ttcactatat tcaaatcagt attccctcaa
     1981 cctttgatgg gatatgcagt atttttatat ggtggattta tgtactattt gattctgatt
     2041 ggaagtggaa tatttactgt aattactcac tatagaatga aaaatagaat tggcagaaat
     2101 attgttttaa gtgaaaacta aataagttta aagaatatat attgagtata ttaaaaaata
     2161 tgtcataaga actagaatat tttaattaaa tggttgaaat ataaagatta ttatgacata
     2221 ttttttttga aatttaagag gaattgtgag aaaattgttg aatttaatag atagtattgc
     2281 aaaatattta tgattttgca tattataaaa acactcatta tctttaagat aaattttact
     2341 tatcagggga taaatctaag gggggaattg tatggagaaa aaaattttaa cagtttgtcc
     2401 atactgtgga gctggatgcc aattatatct agtagtaaaa gataataaga tattaagagc
     2461 tgaaccggct aatggtagaa caaatcaaga aagtttgtgt ttaaaaggtc gttatggatg
     2521 ggattttcta aatgacccta aaattctaac acctaggcta aaaaagccaa tgataagaaa
     2581 acaaggtgta ctagaagagg ttgaatggga tgaggctata aactatacag cttcaagact
     2641 aaatgaaatt aaagaaaaat atggacctga ttctattatg ggaacaggtt ctgcaagagg
     2701 acctggtaat gaagcaaact atataatgca aaaatttatg agagctacaa taggaactaa
     2761 taatgttgac cattgtgctc gtgtctgaca tgcaccatct gtagccggtc tggcttactc
     2821 attaggtagt ggtgcaatgt ctaattctat accagagata gaaaatgcag atgttttatt
     2881 tatatttgga tataatggag cagattcaca tccaatagtg gcaaatagaa tagtaaaagc
     2941 taaaaaaaat ggtgctaaat taattgttac tgaccctaga gtaactgaat ctgcaagaat
     3001 tgctgatata catctaccta taaaaggtgg aacaaacatg gttttagtaa atgcttttgg
     3061 aaatgtatta atagaagaag gtctttacaa taaagaattt gttcaaaatc atacacaggg
     3121 atttgatgag tataaagaaa ttgttaaacc atatacagct aaatatgctg aaaagattac
     3181 aggaatccca gaagagctta taagaaaagc tatgagagaa tatgcaaaag gtaaaaaagc
     3241 tatgatactt tatggaatgg gtgtctgtca atttggtcaa gctgttgatg tagttaaggg
     3301 acttgcatca attgctctac ttacaggtaa ttttggtaga gaaagcgttg gaataggacc
     3361 tgtacgtgga caaaataatg ttcaaggagc ttgtgatatg ggagctcttc ctaatgtata
     3421 tcctggctat caaaatgtaa ctgatgataa gataagagaa aagtttgaaa aagcttgggg
     3481 ggtaaaatta tctcctaata atggttatag tttaactcaa gttccaaatt tagtactaaa
     3541 agagaaaaag cttaaagctt attatatatt tggagaagac ccagtgcaaa gtgacccaga
     3601 tgcatcagaa gttagagaag cattagatga attggaattt gtcattgttc aagatatatt
     3661 tatgaataag acagcacttc acgctgatgt tatacttcca gctacttctt ggggagaaca
     3721 tgagggagta tatacatgtg cagatagagg attccaattg atgagaaaag ctatagagcc
     3781 acaaggagat gtaaaaccag actggcaaat tataagcgaa atttctacag caatgggata
     3841 tcctatgaat tataaaaata ctaaggaaat atgggacgaa cttagacaat tatgtccaag
     3901 tttcttaggt gctacttatg aaaagataga aactcaagga tgcgttcaat ggccttgtaa
     3961 gagtgaaagt atggaagata aaggaactat gtatctgtat gaaggtcaaa agttttctac
     4021 tcctaatggt aaaggtaatt tgttcgctgc tgaatggaga ccccctatgg aagttgaaga
     4081 tgatgaatac ccatttagtt tatgtactgt aagagaagtt ggacactatt cagtaagaac
     4141 aatgactggt aattgtagaa cacttagttc attagaagat gaaccaggac gtgttcaaat
     4201 aaattctaat gatgctgaaa aattaggaat tgaagatgat gaattagtta gaattagttc
     4261 aagaagagga agtgtaataa caagagcaac agttactgat agagttaagg aaggtgcaac
     4321 atatatgact tatcagtggt gggttggagc ttgcaatgaa cttacaatag caaatttaga
     4381 ccctatatct aaaacaccag agtataaata ttgtgctgtt aaacttgaaa aacttgaaga
     4441 ccaagaatta gcagaaaaat gtgtaagaga agagtatcaa agtttgaaag acaaaatgac
     4501 agctacaaat atctaatgat ttattagatt taagatttat aaaaggcaga aaggctaata
     4561 ttttatgaac aatatatact caaaaactaa attacaagaa aatagtaagg ataaagtttt
     4621 tgaaaatagt tatagtctag tggactacaa atatttaaat tcttttaatg tagaagtaag
     4681 caaaataaat gaacatgaag tatcaataga taatgaagaa ataattgcag aatatccact
     4741 tagttttatt ttaaatgata aatatgtaaa tacattttta tgtaccccat ataatttaga
     4801 ggaacttata gtaggattct tgaaaacaaa aggatatata gaaaacaaaa aaagtttatt
     4861 aaaatttgag ataaatgaga gtgaacgttt tgcaaaagta actattcaag agaaacaaaa
     4921 aaatcaagat gagcaaatta tatttttaaa ttcactagac tatataacat gtgctcctgt
     4981 aaagagcaat ataaaaataa attatgatac gatttacagt ataatggata aaaatttaaa
     5041 ttcatcagaa ttatttaaaa atactggtgg ggtacatagc gtatctgtat ttaattttga
     5101 taaaaaaata gtcacttgtg aagatgtagc aaggcataat gcaatggata aggctattgg
     5161 attttgtgtt ttaaatgaca taacattaaa agataaaatt gtggctgtaa gtggaagaat
     5221 atcattggaa atgatattaa aagcagctat gactcaaatt cctattgtag tatcaaaatc
     5281 agcaccgact aatttatcaa tagagctttc taaaaaatta aatataacgc ttattggatt
     5341 tgtaagagga agacggatga atatttatgc aaatccacag agagttatca aaaagtgaga
     5401 atgaatatat gtgtactaaa aaagaaaagc aaagatattc aaagtgtgaa atctgtggag
     5461 tattaattga tgatactgat aaggtgtata ataacacata caatttgtgc aaaaaatgta
     5521 aacgaaatat aagtactgag aaaatagtga aatatataaa tatatatgat gaaagtaagt
     5581 agacatatga tgaaaataac tagagggttg gaaatataca aattgaaagg tgggataata
     5641 tgaaaaataa aataggctct tttgtaatag cagacccaaa caaatgtaca ggttgtagag
     5701 cttgtgaagt agcttgtttt acaatgcata atcagcataa taatgtgggt tatacagttg
     5761 gaacagttga tatacctgtt atacctagac tttacttagt aaaaggtgac aatttctgta
     5821 tgccaataca gtgtagacat tgtgaagatg ctccttgttt aaactcatgt ccacaaaaag
     5881 ctatagttaa agaaaacaat attatgtctg taaatgaaga aaagtgtata ggatgtaaaa
     5941 cttgtctgct agcatgtcct tttggagcta tagacttact tccacaatat gaagatggta
     6001 aagaagtaga acaagtaatt ttagatgaaa ataagaagat agcttataaa tgtgacttat
     6061 gtaaagacaa tgaaaaaata gcttgtataa gtgcatgtcc tcaggaagca ttaaaacttg
     6121 ttactccaat agatgacaaa aaagctaaga atagacaagc tgcacttagt ttattgttaa
     6181 caaataaata aaggatacag gggtaggtga cgtttatgat aataaatata gataaggatt
     6241 tatgtactgg atgtagagaa tgttcaaagg tctgtccagt aaatgctatt gaaggtgaag
     6301 agggaaagcc acaagagata aatttagata gatgtgttat gtgcggtcaa tgtgttcaaa
     6361 cttgtaaatc ttatgcctca gttatagatg aaggatttga gtttttacaa gagaaaaaac
     6421 aagaaagaga gataccagag tctataaatg aacctatatt tgcagcatac aatgtatgtg
     6481 atatagataa ggtgaagaaa gctttaagtg acccaaatgt attcactatg gtccaatgtg
     6541 caccagcagt tagagttgct ttaggagaag atttcggata ttatttagga tacttaggtg
     6601 ctgggaaaat ggcagcagca cttagagctt taaattttga tagagtctac gatactaatt
     6661 ttgcagcaga tttaactata atggaagaag gtagtgaatt aattaaaaga gtgacagaag
     6721 gtggaactct tccaatgttt acatcttgtt gtccagcttg ggtaaaattc atggaaaaaa
     6781 attatcctaa attaactaat catttatctt cttgtaaatc acctcagcag atgggtggtg
     6841 caatatttaa aacttatggt gcagagataa ataatgtaga tgcatcaaag atatttagtg
     6901 tttctataat gccatgtaca tgtaaaaaat atgaatgtga tagagaagaa atggattcta
     6961 gtggatatag agatgtagat gtagtattaa ctactagaga actagcttat ttaataaaag
     7021 acatgggaat tgattttaaa aatttaagag aagaaaaatt tgatagtcct ttaggtagtt
     7081 atagtggagc tggaactatc tttggtgtta ctggtggggt tatggaagct gctataagaa
     7141 caggatatga gcttataact ggagaaagta ttcctgatgt agaagtaaaa caagtaagag
     7201 gagaaaatgg ttttagaaag tcaactatta aaataggaga cttagactta agagttggag
     7261 tagtaagtgg attaaaaaat gtaataccag tgcttgaaga cttggaaaaa ggaaaattag
     7321 atgtagattt tattgaagtt atgacttgtc ctgttgggtg tgtaagtggt ggaggtcaac
     7381 caaaaatttt actagaagaa tataaggaat tggcttatga aaatagaaca aaagcaacat
     7441 acgtgcatga tgaacattta gcaattagaa aatctcatga gaatcctgat attaaaaaac
     7501 tgtatgatga atatctggta gaaccattag gagaaaaatc tcatcatcta ttacacacta
     7561 catattgtat aggaaaggaa gtggctaagt gatgaactat tttgttatag cagaccctga
     7621 taaatgcata ggatgtagaa cttgtatgat aggatgtgta gttgcacatt cagatgaaga
     7681 tattttttac caaaatccag atgaaataaa ttttaatcca aaactttctg tcataaagac
     7741 aaaagaagta agtgctccaa tacagtgtag acattgtgaa gatgctcctt gtgcaaaggc
     7801 atgtccaaat ggtggtatag ttagggttgg taacacaata aaaatcaatg aagaaaattg
     7861 tataggatgt aagacttgta tgttagcatg tcctataggt gcaatagaca tagttacatt
     7921 aaaagatgta gatgaaggaa agctttgctt tagagaaaga atgacagcaa ataagtgtga
     7981 tttttgtata gaatcgccag aagggcctgc atgtgtaaat gtttgtccaa ctaaggcatt
     8041 tactatagtt aaggatgagg atatagatag taaagttaaa aataagagaa aattagctat
     8101 attgtaatat aaaaaagatg gagttttatt tatgattaaa agctccatct tttttattat
     8161 aatatttata tatctaaaaa tcaattattg cattagttta tctaatcttt ctttattcaa
     8221 ttttttcatt cctatagaag ttataataag taaagtatag gcaattataa ctgataaaat
     8281 tataatagac caagaaaatc tgtatccaaa attatcaact aacaatccaa ataatgttga
     8341 acctaatgca aatcccactg caaaaattaa ttgtacgatt ccatatatgc taccaaactc
     8401 cttttgacca aagaaagagc cagttaaata agcaggacca atcatataag caaatacaga
     8461 aagacctttc aaaatggcaa atacaaatgc aaaagtagaa gatttatcta ccaatagtaa
     8521 tgataaagtt gctataagaa ccaatacaaa agatataata agtggctttg tttgaccgaa
     8581 tttatcaaac aataatcctc caaataaatt accaatgaga gcaaataaag caaatgtgga
     8641 gcctactgtt cctattagaa taggatttag ataatttcta agataagttg gatattgtac
     8701 ggataatgat gaaacatata taccaacaaa tataaatcca atagtcataa tccaaaaata
     8761 tttaattttt ttagcttctt ttaatgtata accccatact gaaggagaaa ttgtatcaga
     8821 tgctttctct tttttacctt tagattttac cttttcatta atatctttag gcatcctgag
     8881 tatgaataat attacaggta taccaactat taatgctata attccaaata ttagataaga
     8941 acgactgtat ccttgagttg ctaataagcg aactgcaagt ggttgtagaa atatactacc
     9001 aatagaacca ccagcaaaag caaagcccaa agcttttcct ttgctttctt catcaaacca
     9061 agcacttatt aaaacaggta ttcctatagc agatataatt gtagcgccaa tttgaacaat
     9121 acatgataca gtgtaaaatt gccatagttc agtacatata gaaaaagtag caaaagcaat
     9181 actagaaatt atacaaccta ataaatatag tatcttaata ttgactttag aaaataatac
     9241 acctataaaa ggtgatgcaa ttgctgaggc aatagtacca atagtgaata ttagagaaaa
     9301 agaagctaaa ctaaagcctt ctccttctac aacataactt ataaattgag gctgtatact
     9361 tgctacaata ctaaatggaa cagcttgtat caacatacaa ccaagtacta taacccaagc
     9421 atttgaaaag tttttaatgc taaatttatt gtttagtaat gtattcatgg tatctctcct
     9481 ttaggttatg atttgtagat attaatctaa ttattctaat tttatatatt agttttggat
     9541 agcctaacta tttaaatata atttgggtat taatataaaa attaactata aaaattgatt
     9601 attttaaatg tatggggtaa attaaagtat ataaataaat gaaaggatgg ataaaatgtc
     9661 atttgaaaaa ataaataata ataacctaga taaaatacac aacaggaagt gtattatgct
     9721 tgtaaacttt aataaaaaag aattatcatt aattaaaaat gtaagtaact ttgctggaat
     9781 aagagaccat atcatcttaa gtgataaaaa tggagagaat ataataagag acattttaga
     9841 tggaaatata tcaagtgaat gtgaaaatgg gattaagagt aagtctgtaa tatttaataa
     9901 cactccaagc acacaagtaa atgcattttt agaagggcta aaaaaagtga gaataaatag
     9961 accttttaca gctatggtaa cagagacatc tattgattgg acattgaata atttaataga
    10021 aaatttagtt caagaacgta atgctatgtc tttaggaaaa acaattgaac ataagtaaat
    10081 ttaagatatc ttatagtaat tttaactaat gttcattgag tatcatatta tattttagaa
    10141 tataaaattt taaatgtggg tagatggagg agatttgaga tgaaagtttt atttacaata
    10201 aattatggaa aagaaaaatt tgataaaata agagaattag gctatgatgt catacattac
    10261 tctgaagata gtattgaaaa taatgaggat gtaaatacag cagatgtact agttacatac
    10321 aatccatttg agagattaga tatttctaag atgaaaaatt tacaatatat acagacaact
    10381 agtgtaggta tagaccaact tccaaaagaa gaggtattga agagaaatat taagatagct
    10441 aataatagag gcggatatag tgtacctatt ggtgaaagta ttgtattgta tattttagaa
    10501 atatataaaa atagtgcaaa attttttaaa caacaacaaa ataaagaatg gaagatggat
    10561 ttttctgtat cagaattgta tggacaaaga gttggatttt taggaacagg tactatatct
    10621 tcagaagcag caaaaagatt aaaagctttt ggtgtagaaa tatggggtgt caatactgat
    10681 ggaagtagta aaaaatactt tgataagtgt tttgctactg aggatatgaa tattgtattt
    10741 gaagaatgtg atatagtagt tgtagcaatg ccttctacaa aaagtacaga tggaatagta
    10801 aacaaagata tgtttaaact tatgaaagat ggttctgtat ttataaatgt aggtagagga
    10861 aatacagtta atcagaaaga tttagaagaa tgtgtagaga aatttagagg ggtagcatta
    10921 gacgtatttg aaagtgaacc tttaaacaag gacaataagt tgtgggagtg cgaaaatgtc
    10981 atagtaactc ctcataattc ttgggtgtca gataaaaaca aagagagaac atttaatatg
    11041 gtttataata atttgaaaca ctatataatg gagaataaac ctgtagataa tgttgtagac
    11101 atatcaagag gctattaaaa ataaaagatt ttgataaaaa taaaaactct tattagtcgt
    11161 attaaagata ctaataagag ttttttttgt aataaattta aagccaaaaa agtgatatac
    11221 taaaaaatat ataaatagga gataattaat tatagggaaa tttcaaaata atttaatttt
    11281 aaaaatatga agaggtgcaa aaagtgagcg taaaagatga aattaataaa cttatagaga
    11341 caataaacta ccataatgaa aggtattata atcaagatag cccagaaata tcagattatg
    11401 aatacgataa gttaatgaag gagttaattt ctttagaaga agaacatcca gaactaaaga
    11461 gaattgattc tccaacaaat agggtaggag gtaagccatt agataagttc aatcaaattg
    11521 tacataaaac tcctatgttg agtttatcaa atgctttttc agagcaagat ttgagagatt
    11581 ttgataagag agttcaagaa tatgtgggag aaaatgttga atatgttgtt gaatttaaaa
    11641 tagatggtct ttctgtaggt ctaacttata acaatggaga gtttgaaaaa ggagcaacta
    11701 gaggagatgg agttgtagga gaagatattt ctcaaaactt aatgacagta aaaagcatac
    11761 ctcttaagat aaatgataaa aaagaactaa tagttagagg agaagtatat atatcaaaag
    11821 ataattttag aaaagtaaat gaacaacaag aagaacaaga acagccatta tttgctaatc
    11881 caaggaatct tgcagctggt tcattgagac aattagaccc aaaactaaca gcaaagagac
    11941 cattagatat atttgtattt aatatggaaa atatagaaga atacgattta gaaagtcaca
    12001 gtgaatcact agagtactta gaaaaactag gattttcagt aagtcagagt tataaagttt
    12061 gtaaaagtat agatgaagtt attgagcata ttgaatactg gacagataac aggggtaatt
    12121 tagactacga aatagatggt atggtaataa aagtaaacaa tataaaacaa agagacgaaa
    12181 tgggatatac agccaaaagt cctagatggg caattgcata taaattccca gcagaaaaga
    12241 agaaaactaa aatagtagat ataatagtag aagttgggag aactggtaca ataacacctt
    12301 cagcaattct tgaaccagtc agacttgcag gtactactgt aagtagagct acacttcaca
    12361 atgaagatta tataagagaa aaagatataa aaataaatga tacagtatta gttcaaaaag
    12421 caggggacat aatacctcaa gttttagaag ttataaaaga agaacgtact ggagatgaaa
    12481 taaattttaa aatgccagaa aagtgtccag tttgttcaga gccaacaata agattagaag
    12541 gagaggctgc tgtaaaatgt atcaatatgt cttgcccagc ccaaataaga aggggaatta
    12601 tacactttgt gtcaagggat gcaatgaata tagatggatt gggagaatct ataattacac
    12661 tgctattaaa tgaaaaaatt attcaagatg tatctgattt atattatata aaaaaggaag
    12721 atgtagtcga cctagagaga atgggcgaaa aatctgctga caatttaata aattcaattg
    12781 aaaaatcaaa agaaaatgat ttatggagac ttataaatgg attaggtatt aaatttatag
    12841 gagttaaagc agctaagata cttgcatata actttaagga tttagatgaa gtaatttctg
    12901 caactagtga gcaactagaa gaattagagg aatttggaag tataatggca aacagcgttg
    12961 tcgagttttt taaggaagac aaaaatatga acgttataaa caagttaaaa aatgtaggag
    13021 taaatacaaa atcattggat aatacaggtg aaaaagtaga aaaaatattt gaagggatga
    13081 aaatcgtatt gactggtaca cttcctacat taaaaagaaa tgatgcaaaa gaaatgatag
    13141 aaaaaagagg tggaaaagct acatctagtg ttagtaaatc gacaacattt gttttggcag
    13201 gtgaagaagc tggttctaaa cttactaaag caaatgattt aggaattaaa gttatagatg
    13261 aagagagatt tttagaatta ttaaagttat catcgaaaga tgaagtaaaa gctgtattag
    13321 agagttaaaa agtcgcttaa tttagtagaa aactactata attaatgata gaatagttca
    13381 gtaagctaat aagattacga aaaatgacaa taattaatat ttgacaaaat taaaaattat
    13441 aaaaataaat ttataaaaaa taaaaaatat gagaaaggat ataaaaaatg accagaagtt
    13501 atgatagaag taatgaacag ataagacctg taaagataac tagagattat acaaagtatg
    13561 cagaaggttc tgttcttata gaaatgggag agacaaaggt tatatgcact gcatcagtgg
    13621 aagaaaaagt tcctcctttt ttaagaaata gtggtactgg atggataaat gctgaatatt
    13681 ctatgctacc aagagcaaca caacaaagaa agataagaga tgcatctagg ggaaaaattg
    13741 atggaagaac acaagaaata cagaggttaa taggaagagc aataaggtct gttgtagact
    13801 tgaataaatt aggagaaaga actatttggg tagattgtga tgtgattcag gcagatggtg
    13861 gaactagaac tgcatctata acaggggcat ttgtagcagt tgcagaagca atatataaat
    13921 tatataaagc aaagacaata aaagagatgc caataacaaa ttttgtatct gcaattagtg
    13981 ttggtattgt aaatggaata cacatgctag atttatgtta tgaagaagat tctaaagcac
    14041 aggtagatat gaatataata atgactgata agtgtgagtt tgtagaagta caggggacag
    14101 gagaagagaa acctttttcg agagcagatt taaatttatt gttagagtta ggtgaaaaag
    14161 ggaataaaga attaataagg cttcaaagag aagctttagg tgaaatatca gatgagatat
    14221 tgggtatgga ttatggtaat gaagtagtga ttgctactaa taatgctcat aagctagaag
    14281 aaataggtga aatcttaaaa gattttgaat ataaagtgta ctctttaaaa gatgttgatt
    14341 tagcaggtat agagatagta gaagatggaa aaacatttga gcataatgcg ttgataaaag
    14401 ctagaacaat agcaaaaaag actaaactta tagcaatatc agatgattct ggcttagaag
    14461 tggatgcttt aggcaaaaaa cctggtgttt attcagctag atatgcagga gaacatgcaa
    14521 cagatgaaga aaatagaaaa aaactgttaa aagctatgca aaatgtacca atgagcaaaa
    14581 gaaatgcaag attcgtctct gcaatagcag tagtttttcc tgatggaaaa gaatttgtag
    14641 taagaggaat ttgtgaagga atgataggat ttgaagaaaa aggtaaaaat ggttttggat
    14701 atgatagctt atttatagta aaaggatatg ataagacttt tggtgaaata cctagtgtta
    14761 taaaaaattc aatcagtcat agagcaaacg cactaaaact tatgaaacaa gagtttataa
    14821 aaagggtagt gaagtaatga aaataggcat agtaagcgac acacatatga tgattaaaaa
    14881 tatggaaaaa acaataccat atttgaaaga atgtgaccta ataattcatg caggagataa
    14941 ctttactgat tcaagatata ttcatagcat gactaatgta ggtattattg cagttaaagg
    15001 taattgtgac tttgatgctg ttgaggaaga agttgtcttt gaagtggcaa ataaaaccat
    15061 attcttatgc catggagata agtatggtgt aaaatatgga actaatatgt tagaaaaaaa
    15121 ggcaactgaa gttgatgctg atatagttat atttggccat acccataccc catttagaga
    15181 aattaaagat ggtgtacttt acataaatcc tggaagcact tcacttccaa gaggagtatc
    15241 gtataaaagt tttgtcatta tggatataga agaagatgac ataaaaattg aagaaatacg
    15301 catataaagg tgtatttatc taaaaaaatg gcaaaaaacg ctcaaaaaat taaattttat
    15361 caaataagta caacgatatt aatggaacac ttgtaaataa aaaaacttat gtgatataat
    15421 taagtggttg aattataaat agaatgaatt ataataccgt tatcgggtat tacataaata
    15481 tcactaggag gattagaagg atgaaagcag aactaattaa aaaagagggt aataaagtta
    15541 ctttaaaaat aacagtagat aacaataaat ttgaagcagc agtaaataag gcttataata
    15601 aaagcagaaa taaatacaat atacctggat ttagaaaagg taaagctcca agaattgtta
    15661 tagaaactca atacggaaaa ggtatattct acaatgatgc tatagaaata ttattcccag
    15721 aagtttatcc agaagctatc aaggaattag acatagaccc aatagacaat ccagatttag
    15781 atatagaaga aataagtaaa gacaatgggt tggttatggt tcttaatgta gaagttaaac
    15841 cagagtttga attaggaaac tacaagggta tagaaatagc taaggttgag aatactgtaa
    15901 gtgacgaaaa tgttgatgct aaactacaag aaatggttga aaaaaatgcc agattagtaa
    15961 gtgtagaaga caaagcttta gaagatggag atacagctat aatagacttc gaaggttttg
    16021 aaaatggtgt agcttttgat ggtggtaaag gtgaaaatta taacttagtt ataggttcaa
    16081 acacatttat accaggattt gaagaacaat tagtaggtaa aaaagctggt gaagaagtag
    16141 aagttaatgt tactttccca gaagaatatc actcacaaga tttagctgga aaaccagttg
    16201 tatttaatgt aaaaataaat gatgtaaaag taaaagaatt atctgcactt gatgatgaat
    16261 ttgctaaaga tactagtgaa tttgattctt tagatgaatt aaaagcagat gtaagagcta
    16321 agttagaaga agaagctaaa aatagagcag atgcagaaac tagaaattca gtagtagaaa
    16381 aagttgctga aaatacagaa atagaaatac cagaagttat gatacaacat caaatagata
    16441 atatgttaaa tgaacttaac taccaattac aatatcaagg atttggatta caacaattac
    16501 tagagatgac tggtagaact atggaagaat taagagaaga gagaaaagaa gatgctaaaa
    16561 aattagttaa gtcttcttta gtattagaag caataactaa agctgaagga attgaagcaa
    16621 cagaagaaga atttaaggct gagttagaaa aaatggcttc tgcttacaat atggaagtag
    16681 aaaaaataga agcttcttta agagatgctg acaaagaaga tataaaaggt caaataaaaa
    16741 taagaaaaac aatagattta ttagttgata atgcaacaat agcttaataa aataaacgct
    16801 aactaatata gttgacgtta tgaaatgttt agtatataat gatgtgcaaa tataatttag
    16861 ttaaatgcgt ataataattt acagtagctt aaaaaaagct actgtaaatt atatagaata
    16921 aagggggtat gaaaccatgg cattagtacc tgtagttgtt gaacaaacag gaagaggaga
    16981 aagatcttat gatatttttt ctagactatt aaaagacaga ataatcttct taggagatca
    17041 agttaacgat gccacagcag ggcttatagt agcacagcta ttatttttag aagctgaaga
    17101 cccagacaaa gatatacatt tatatataaa ttctccagga ggaagtataa cttctggaat
    17161 ggctatatat gatactatgc aatacatcaa accagatgta agtactatat gtataggtat
    17221 ggctgcttct atgggagcat tcttgttggc agcaggagca aaaggaaaaa gactagcact
    17281 accaaatagt gaaataatga tacatcaacc attaggtgga gctcaaggtc aagcaacaga
    17341 tatagaaatt catgcaaaga gaattttaaa aataaaagaa actttaaatg agatattatc
    17401 tgaaagaact ggtcagccat tagaaaaaat taaaatggat actgaacgtg ataacttcat
    17461 gagtgcacta gaagccaaag aatatggttt aatagatgaa gtatttacaa agagaccgta
    17521 gatagagaga ggtgttaata tgtctaaata tgaagaaaaa cgacaattaa agtgttcatt
    17581 ctgcggtaaa aatcaagatc aagtaagaag attaattgca ggtccaaatg tatatatttg
    17641 tgacgagtgt gttgaacttt gtgacgaaat tattcaggaa gaaatagaag atactataga
    17701 tgaagatact acatctttac caaaaccaaa ggaaatgatg gagattctaa atgattatgt
    17761 aataggtcaa gaaaaagcta aaaaagcttt atcagttgcg gtttataatc attataagag
    17821 aatatacagt aaaaaatcta gtagtaagga tatagaaatt caaaagagta atatactttt
    17881 attaggacct acaggttctg gaaagacatt acttgctcaa actctagcaa gaactctaaa
    17941 tgtacctttt gcaatggcag atgctacatc attgactgaa gctggatatg ttggagaaga
    18001 cgttgaaaat atacttttaa aacttattca atctgctgat tttgatatag aaaaagctga
    18061 aagaggtata atatatatag atgagataga taagattgca agaaaatctg aaaacccatc
    18121 tataactaga gatgttagtg gtgaaggtgt tcaacaagca cttctaaaaa tattagaagg
    18181 tactgttgca aatgttccac cacaaggagg aagaaaacat cctcatcaag aatttttaaa
    18241 gatagatact actaatgtat tatttatatt aggtggagct tttgatggat tagagaagat
    18301 aattcaaaaa cgtggtggag ataaaacact aggttttgga gctaaaatag aaagtaaaaa
    18361 agaattagat ttagggaagc tatatgaaaa agtacttcca gaggatttat taaaatatgg
    18421 tataatacca gagtttatag gaagaatacc agttttagct actttagagt tgttggatga
    18481 agatgcatta atgcagatat tacaggaacc taaaaatgct ctagtaaaac agtataaaaa
    18541 gttattggag ttagatgatg ttgagctaga atttgaagaa ggtgctcttc gtgcaatcgc
    18601 taaaaaggcc atagaaagaa atacaggagc tagaggactt agaagtatag ttgaaagcgt
    18661 aatgatggaa actatgtttg aagtaccatc tagagataat ataaagaagg ttatagtaac
    18721 agagaaatct gtgaacgaag actcagtaaa tcctatcata gtacttaagg atcaagagga
    18781 aagtgcataa gatgatgaaa aggagcttta aaagctcctt tttttatgtt aatatgctaa
    18841 atataaaata agtatcatta ttataaaaat gtgagtaata tacctagaat accaaagacg
    18901 aatacaattg caatggaacc aatttttcct ttatataaaa gataaaatga tgctataaat
    18961 gcaattattc catataagtc ccaattaact tctttggcag atgaaaaagc tgctgataat
    19021 atgaatccta gagtaattgg tctaagaact gtcaataagt tttctacctt tttattattt
    19081 ttaaatttag aaaataactt tgatataata gttagaccta ttaatgcaaa aattgtaaca
    19141 ccaatagttg cagctagaga accaaagaag ccagcagtct taaatcctac aaatgtagca
    19201 ctatttattg ctacaggacc tggtgtagat tgtgatatag caagtaaatc caaaaactct
    19261 tgattagtta taaagtgata tttagatata aattcttgct ggataaatgg tagcatggca
    19321 taaccgccac caaaactaaa tgcccctatt ttaaggaata ttaagaagag tagtattatt
    19381 tctgacatag actatataca cctcctccaa ttattaaata aatagggctt atattgaata
    19441 caccaacaga taaaactgct ataattaata gtagtatgtt agttttagac tttggtagtt
    19501 tcttgaacat gctcgtaaaa gaatacaata taagagctat tacaacaggc gcaactcctt
    19561 taaaaaatcc atctaaaatt ttagatgatg aatttttaaa gtatagatac gataagacta
    19621 agactataca aaatgatggt aaagcagagc ctaaggtaca tataagaact cctggtatac
    19681 cataaagttt atatcctaat aatacagata tattaactgc cagtactcca ggataacttt
    19741 gagctatgga taaataatca ataaattctt cctgctctaa aagtttttgt ttgtttacaa
    19801 gttcatcttg tataagagga accatagcgt atccaccacc aaatgtaaac gcaccaattt
    19861 ttaaaaaggt taaaaacaat ttcaacataa tagaaacctc caaaagataa ataacatcta
    19921 agtatactaa tattaatatt atatcataat aaaaagataa tatagtatga tagtattcta
    19981 agaatttatc attaatgttg aaaattaact tgttcaaata tataatatta aaagcaaagc
    20041 aattagacta gaaaaggagt aatgttgatg gaacaaaatt atactaaaat agatcatgaa
    20101 ttaccattaa ttcctctaag aggattagct atatttccat atatgatatt aaattttgat
    20161 ataggtagag agatatctct taaagcttta gaccaagcta tgatggatga agaacttata
    20221 tttttaactt ctcagaaaga agcagaggta gatgagccag gagaagagga tttttatcat
    20281 gtgggaacaa tctgtaaagt taagcaaatg ataaaattac caggagatac agttcgtgtt
    20341 ttggttgaag gtgtatcaag gggtagagta aagaaaatag aacaagaaga tgggtatttc
    20401 agagcagtaa tagaagaaat agtatttgat agtgataatt tggatagtga aacagaagtt
    20461 gaaattgaag catttgtaag aaatgtattt gatgcctttg aagagtatat aaatattggc
    20521 aatagggtat caccagaaat attaatatct ttagctgata tagaagatgt agatagattt
    20581 atagatacga tagctgcaaa catatatcta aaatcaagcc aaaaacaaga aatattagaa
    20641 gaatttgata ttagaaaaag acttgaactt atttattcta tattattaga ggaaatagat
    20701 atattaaaaa tagaaaagaa aataacttta agagtaaaaa aacagatgaa taaggttcaa
    20761 aaagaatatt atcttagaga acaattaaaa gctatacaga aagaattagg tgaagaagaa
    20821 gatataaact ctgaagcaga tgagtataga gaaaagctta aaaaaataaa agcacctaag
    20881 acaactaaag agaaaataga aaaagagata gataaatttt caaaaatatc ttctatgtct
    20941 cctgatgtat ctgtaagtag aaactattta gatactatat tctcattgcc atggaataaa
    21001 gagacaaaag ataaattaga tataactaag gcaaaagata tattagatga agaccattat
    21061 ggattagaaa aagttaagga gagaatacta gagtatctag ctataagaac tcttgctaag
    21121 tcacttaaag gtcctataat atgtctagtt ggtcctccag gaacaggaaa gacatctata
    21181 gtaaaatcaa tcgccagagc attaaataga aaatttgtaa gaatatcttt gggtggagtt
    21241 agagatgaag cagaaataag aggtcataga agaacttatg taggttcaat tccaggaaga
    21301 ataattaatg gggtaaaaga agcccaaaca aaaaatcctg tattcttatt tgatgagata
    21361 gataagatgg cagcagatta taaaggtgac ccagcttcag caatgctaga agtattagac
    21421 ccagagcaaa ataaggattt tgtggaccat tacttagaaa taccttttga tttatcaaaa
    21481 atactatttg ttacaacagc aaatagtcta ggtaatatac caagaccatt attagataga
    21541 atggaagtaa tagaagtatc tgggtatata gaagaagaaa aattaaatat agcaaaaaaa
    21601 tacctacttc caaaacagat taaagaacat gctttaaaag aaagctttat aaaaatagat
    21661 gatgaaactt taagaagtat aatcaaccat tatacaagag aagctggagt aagaacacta
    21721 gaaagaacta ttggtaagat ttgtagaaag gttgccaaaa aatacgttga agacccaact
    21781 cttgaagagg ttgttataaa taagtctgac ctagagacat atcttggaaa agacatgttt
    21841 aagtatcaac ttgcagaagt taaccctcaa ataggattag taaatggcct tgcatggaca
    21901 gaagttggtg gagttacact tgaagtagaa gtaaacgtac ttaaaggtaa aggagaaatt
    21961 gtattaactg gaaaattagg agatgttatg aaggaatctg cgaagacagg tatctcttat
    22021 ataaggtcaa tagtggacaa gtttgatata gaccctgaat tttataagac gaatgatatt
    22081 catatacaca ttccagaagg tgctgttcca aaagatggac catcagctgg tataactatg
    22141 gctcttgcag taatctcagc acttactaaa agacctgtgc ctggaaatat agcaatgaca
    22201 ggagagataa ctttaagagg tagagtctta gcagtaggag gagttaaaga aaagttatta
    22261 gcagctcata gagctggtat aacaaaagta ttaattccta aagaatgtga agctgattta
    22321 gatgaaatac cagaaaatgt aaaagaaaaa atggagtttg tgcttgtaga acatatggat
    22381 gaagtattag aacaagcttt attaaagagt ggtgaaaata atgaaaatta gaagttcaga
    22441 gataactatg agtgctgtga ataaatcaca gtatccagca gaaggaatcc cagagatagc
    22501 attggctggt aggtcaaatg ttggaaaatc atcaataata aatacattgc ttaacagaag
    22561 aaactttgct agaaccagtc aaacaccagg aaaaactaga actataaatt tttatctaat
    22621 aaataatgaa ttttattttg tagaccttcc aggctatgga tatgcaaaga tagctaagtc
    22681 tgaaaaagaa aagtggggag gtataatgga aagatatcta gaaagtagac aagagttatg
    22741 ttctatattc ctacttgtag atattagaca tgagcctaca gcagatgata agcttatgta
    22801 tgaatggata aagcactttg gatataactg tgtagttatt gcaactaagg cagataaaat
    22861 atctagagga caatatcaaa aacacataag tataataaga aaaaaattgc aaatggaaag
    22921 tagtgaaaaa gtaattcctg tttcttcact aaaaaagact ggtgttgaag aattatggga
    22981 agagattgta aatcaataca atcaacatgg atatgaaata acagtagatt aaatttaaag
    23041 tgttaaatat tgacataaga tttgtttgaa taataaatgt aataatagtt agttttaatt
    23101 atattaatat taaagcaaaa tataggatgc cttaaaatag ttttaatgaa ctttttttgg
    23161 catccttatt gtttataaac aataaatatc agtaattttg ctaaatacta ataaatttta
    23221 gtaaggtatg ggaaaattag tataaattag ttcaactaaa aaattattga cttatgtagt
    23281 cagcaataaa tagcatttaa gaaatagatt tatctgtttt tctcataaag aaaacagtta
    23341 gtataagccc tataaaacat actgatgctg caatccaata agactgattg ctagctataa
    23401 cataggctga tgtgtgactt ttaaactttt gattgtatgt aagactttgt gcaaaaaaac
    23461 tactaaaaaa tgctgttcca agagcaaatg ataaattacg aatagttgca agaaaactgc
    23521 ctccgtatcc ctgtttttga ggaggaacat ctgctaaaat actactattg tttggtgaac
    23581 taaatgctcc tagcccaagt ccaaaaataa ctaaacatac aattaaaaga tacaatggag
    23641 aatctgcttt taaaagtgcc attccaacta gtgagatggt cataaatgaa aaagatgctg
    23701 ttagaatgtt ttttgcaccg tgtttatcag aaagtgaacc tgcaataggt gctataagta
    23761 ccataataag tggatgtaca gtcattacat aaccagccat cataggagat aaatttttta
    23821 attgttctaa ataaaatgga agtagaaaag atactgccat ttgaggaaaa taacaagcta
    23881 ctccaatgat attgcctaca gtaaagtttc tatttttaaa tagaggtagg tcaataaagc
    23941 tttgttcaaa ttttacttct cttaatgcca acaataagaa gattataagt gctgttatac
    24001 ctaaatacaa tcttgtatta cccttctcat tcattgcaag taatataagc gtagtgcaag
    24061 atgcgaaaag tagaagacct ggaatatcaa agctcttgtt ttgttcatcc aaaacaggtt
    24121 taggcagaaa tttaatgcca agaaagactg ctataatacc aataggtata ttgataataa
    24181 aaatagaagg ccatccaaaa tgttctaaaa ttatacctcc aattacagga ccactcatat
    24241 ttcctatacc aactacagct ccagtgattc ctatagcttt tcctttttca tttgctggaa
    24301 atgcattgga aactatacca agcccagtag ccattaaaat acttgctcca actgcctgaa
    24361 agattcttga agaaattaat gttgataaat tgtttgacat actacacaga aaagaaccta
    24421 ttgtaaatat aaaaaatcct aatgtgtata gacgatttga acctacttta tcgccaagtt
    24481 ttccgaaaaa taacatagta gctgttacaa caagcatata agctgttaca acccactgaa
    24541 tttgtgacat gttaatttta aagtcagcag caattgtagg atttgcgata tttagtatac
    24601 ttccatcaag agtagacata aatacaccaa tacagatgat aatcaatact atccattttt
    24661 gtttcatgat aacactccaa tcttaataga ttttataagt acgatataat tgtaaaataa
    24721 acaacaaaaa aagcacctgt atattaggta ccttgaatgt gtctaatttc atgaataaat
    24781 acggtagttg aaggatttgt atagattact catgtaaaat tacataaagg tactcatata
    24841 ctattttctt ctaccatcca gattttactg ttggctttgg aatatgacca aattatgcca
    24901 taaggctcgc gggcttttac cgccgatagg gaatttcacc cgtttatgaa gaaaaacaga
    24961 tgtattttgt aatattattt taacataaaa ttataatgga aacaattgga tttaagtaat
    25021 atccagctgt attacaatac aaatatttca atatggacac ttaaattgta tttatagata
    25081 gaataaggca cagtatgaga atctataata attagataaa attaacctgt tattagtatt
    25141 agatggcaag ttttaaattt gtcaaaatca gaaagctgta ataaaattct tataatatga
    25201 atatcttaat taagagatat tgtaaattat gaggaggcgc taatatggaa ggaaaggtaa
    25261 agaaggcatt cccgggtgga aatacatcac aaggctcata ttcttttttt gactatataa
    25321 ttcctgaaaa tgtaaaaaga gttttttgtt taaagggtgg acctggagta ggaaaatcct
    25381 cacttatgaa aaaaatttat aaagagtttt tgcatagagg atatgatatt gatttatatc
    25441 attgtccttc tgacccaagt tcgttggatg gtttagttat aaagaaatta ggcgtggtac
    25501 ttatggatgc aactgcccca catactatgg acccaaagct acctggagct ttggatgaga
    25561 taattaattt aggtgattac tggaatacag aagaattaga aaagaataaa caagaaatat
    25621 cagtttatga taaagatatt agtaattcat ttaaaagagc atttaagttt ttaaaatcag
    25681 cagaaccaat ctttagagac atagaagaaa aatattctga ttgtatggat tatggcaaag
    25741 taaatttggt cacagaagaa tttataaaaa ggctatttga tggagttaaa agcacaggaa
    25801 atctaaaaag agaaaagcat ttatttggtt cagctataac tcctgtaggg tgtttagatt
    25861 atacagaaaa cattttaaaa gatgtaaaga aagtctatta tttagatgga gaaataggaa
    25921 ctgggaaaac tacattatta aacaaagtat ataagaaagc tattgaaaaa ggtttaaatg
    25981 tagaagttta tcattatcca cttattccag agaaaattga aactgtacta ttaactgatt
    26041 tagatatagg cataactata agtacgactt ttaaagaaga agatacaatt gatttaaatc
    26101 agtttttaaa tagagaaaaa ctacttaaat atgaagaaga tattaaattt gatgaaaaag
    26161 ttttagatga tttaatttgt tgggcagtac taaatcttaa gagagccaag tctaaacatg
    26221 atattataga aagttactat agtcctaata tgagatttga tgaagtaggg aaattaagag
    26281 ataagttaat taaaagaatt ttaaaatatg aggaaaactt ataaacgtat atgtttaaaa
    26341 ttaacgttaa taataatata atttttagta aattggtgta gctttgaggc tatgccattt
    26401 tacttttttt tgctaaaaaa tgatattaat aatctccaaa gccattattt gtttttcata
    26461 attattattt cctttcctaa tttatgtttt tttataatat cacaacaatg tttattgaaa
    26521 gtttatataa ttttaagaac atctaaaaat gcatacttga tattggtaaa ttagtgatag
    26581 gagttcaatg tatgatatta tatactaaag atgaatttta atatataact ttgatataat
    26641 aagcatatgt aaatgtttaa tatttaatta aagaaaatta tcatataaaa ataaatttgt
    26701 aaggagcaat acagtatgaa aaaacaatat gtgtttataa tactagcaag tactatttta
    26761 ggtatacttg ttggaaccat atttagagat tatagcaatg aagaaacagt ttatacaagc
    26821 aaagacaagt caataagaaa agaaataaaa atgacaagaa aatctataaa agaattaacc
    26881 aaagagaaag acaagttaga taaagaaatt aattcaatga aacaaaatca aaaaaattct
    26941 gaagaaatat cagaaataga aacacttaga gatagtctat catattcaga tgtaaagggt
    27001 agtgggataa tattaagtat agatgctata gatgaaaaaa cagggaatat agctaatagt
    27061 atagactata ataagattct tgtaaaagtt ataaatgaat taaaagtaaa cggaggaaaa
    27121 tacatagaaa taaatggtca aagaattaat caatattcag aagttgtact tgcaggaagc
    27181 catataaata taaatggagt tcctgtagca caaccatatg aaataaagtg cattggagat
    27241 aaggagaaac tatctaatta tgtagataaa ggtagtaaat atttagatag catacaaacc
    27301 aattatccaa tgaaaataag cataaaaact gaagaaaata tctctatgaa aaaaatgaat
    27361 atacctaata aattaaagta tataaagggt gattaagatt atattagagg aaagcagttt
    27421 gccaatgtta ataccagaaa aacttgcaaa aaaatataat gtatttccag ttgagtttca
    27481 agatgataat ttagttgtag aaatagaaga tgaggatatt tatgctctac aggacttgag
    27541 attagctaca ggtaaagaaa ttatattgaa gaaggagaaa aaagaactta taagtgaaaa
    27601 gattgaaaaa tattataata gctttgaact tgatgaggat tatgcaaaaa aattatttga
    27661 aaatctttta gaaaaagcta taaaagaaaa cgcaagtgat atacatatag aaccatttaa
    27721 agaacattta atagtgagaa tgagggtaga tggagaacta aaggaagtat caaagttttt
    27781 agttgatgtc tatccatcat tgtctacggt tataaaatta aaggcatcaa tggatataac
    27841 agaaaaaagg cttcctcagg atggtagggt ggatataaag ttaggtggaa atttaattga
    27901 cattagagtt tcatcaatac caactgtcta tggtgaaaaa atagttttga ggatacttaa
    27961 tagagatact tttttaaaag acaaattaga acttggtttt tcagaggaag ctataaaatc
    28021 tataaatcgt ataataaata aaagagcagg aatattatta gtgacaggtc ctacaggtag
    28081 tggaaagact acaacagtat attccttatt aaatgattta aaaggaataa ataaaaatat
    28141 tatgactata gaaaatcctg tagagtataa gatggatggc ataagtcaga ttcaagtaaa
    28201 ttcaaaagta gggttgagct ttgatgtagg tcttagagcg atattaagac aagacccaga
    28261 tataataatg gtcggagaaa taagagatgc agaaacagct aagatagcag taagagcagc
    28321 tataacaggt catttagtta taagtacttt acatactaat gatgcagtat cttccatagc
    28381 tagattacta gaaatgcaaa taccaccata tcttttaaat gcctctctta taggtgttat
    28441 atctcaaaag ttggttagaa aagtctgcaa tcattgtagc catgagatta tgataaaaga
    28501 taattttagt ggagatgtaa atactaaggt agctgtaggg tgtgaaaaat gtaatgataa
    28561 aggatacttt ggaagaacag caatttatga aatcttagaa attaatgatg atataaagac
    28621 atgtataaga aatatggagg attctagtac tattaaagat gtggcacaaa ataatggaat
    28681 gataacattt gaagatagtt gtaaaagact aataaatgaa aaaataacga cattagaaga
    28741 gtgtttagta gtaaatgaaa tgatttaact gaattgtata gttaaacttc gtttggtcag
    28801 ttgtctttat ttaatctatt ttaattaaga tgtttgagta taaattatat ttaagtgaaa
    28861 taaaattaaa tgcattaaat tagaaaatat taagtttatt taattgtaat taatataatt
    28921 tggaaggtat tatagtacat gagattatct aaaaatatat tttataattt agaaaagttt
    28981 tatttagtat taaatagaat cagtgataaa gaaattagag taatttgcaa agagataggc
    29041 atattgctgg aatctggatg tgaaattaca aaaatatttg aaatcattga gagtcagtca
    29101 agtaaaaaag taaagaattt gcttagtata gtatcaaatc atattcaaaa aggtaactca
    29161 atagcagaat catttcaaat aacaggtata ttttctaaat tctttataag tatgataaaa
    29221 gcaggagaaa ctagtggaaa cttagatata ataatgagtg atttatctaa ttattatgat
    29281 aaagaataca aattaaaaat gaaaataata actatttcaa tatatccaat aatacttata
    29341 atattatcta tcttatcaat gttgttcata ttcgtatttg taatacctaa ttttcaggtg
    29401 gtttttacca acaatggaat tgaaccacct ttaataacta gagttttaat gagaatatct
    29461 acagtggtta caaataactt agcttatata atatttagtt ttatattgtt ttcaatagga
    29521 agcatttatt tctttataac aaatgataat ataaaaaaat tgattaattc gttaaaattc
    29581 aaaatacctt ttataaaaaa aattaatcag ttagtagcta caacaagatt ttcaagggca
    29641 ttgtccgttt taattagcag tggaatacaa attgtggaag ctatagacat gtcagctagt
    29701 gttatagaca acgaatttat ttatgaaagg ttaaagattt ccaataatta tataaaaaaa
    29761 ggaaatagca taggtaaatc tttgaattta gcaaatgttt ttccaaaatt atttatttca
    29821 atggcaaata caggagaaga aagtgggaaa ctagataaaa gtctaaatgt aataaataaa
    29881 ttttatgaaa atgaattaaa taacaaaata gagcaaataa tgaaatttat agaacctatg
    29941 ctaattgtat ttatgggatt aattattggt acttttattt tagcaatggt tgttcctatg
    30001 tttgatgcaa ttactaattt ttagagagaa aaataaattt tattagatga aacttttgaa
    30061 aaggagagaa tatttaattg ataaatttga taaataaaaa acgaaaagga tttacacttg
    30121 ttgaaatgat tgtagtagta actattttag gcgttatatc tagtatagca ttagttaagt
    30181 atagtaaggt tcaagaaagt gccaaattaa atgcagacta tacgaatgct gctaatatag
    30241 taaccgcagc tagcatggca attaatgatg atgaaaagac aatagactct ctaagtgtag
    30301 agacattgaa ggaaaaggga tacctaaata ctgttccagt tcctcagagt acatcaggta
    30361 aattcgaact tgtcataaat gatagcggaa cagatataag cgtaaatata aattctaagc
    30421 aattttatcc aaaataatat tttatattga ttgctgtaag ttttaaaatt atttttattt
    30481 aagaaggaga tggctataat taaaaaaatt ctaaacttag aaataaatga tgattatata
    30541 aggcttgtat ttttaagaaa aggtcataaa aaagttttta tagataaatc aattataaaa
    30601 gagattaact ttgatataaa aaatgatagt aattcgattg agaaggttaa atatatagaa
    30661 gatgtagtta gatttgtaaa agaggagatt ggtaaatcaa aaatattata taaaaattta
    30721 tactttaaca ttcaaattca ggatatagtc attagaaatg ttgaagttct aaatactaag
    30781 aaaaaaagag acatattaag catgattgaa tttgaaataa cacaatacat tccaataaac
    30841 ataaataact attcaataaa atataaagtt ataaattcta aaaatgataa attgaatata
    30901 caagtaattc ttttcccaaa gagtattatt caattaatta agaacatttc agaaaatcta
    30961 aatatgaatc caaagactat aaatataaat tttgatatat tacaaaagct tataaactta
    31021 aatttaatag agaatttttc agaagaaggg gtatttatag aatgtaaaaa aagtcagtta
    31081 attataaata taacaaaaga taaaaaaata agagaaacat acttgctatc aaaagaggga
    31141 aaatcatatg agttcataaa aagattatta agggatttta agtatgttta ttactatggt
    31201 atagaaaatt gttttattga agaatacttt aaatatacag ataaatctat tgaagtaaat
    31261 ccattaagat taaaagataa agtaaaagta gaaaataatt ttgatgcaga tttagaagac
    31321 aataggataa attatataaa tagtatagga atgattattt aagtgaaagt ggataaaaca
    31381 ataaattttc ttaatggatt taaaaaaact aaaccatgta ataaaatcta taaaataata
    31441 atagtcttaa ttttaacgtg tattttattt aaatcattta tgtatgtgaa atctataaat
    31501 gaacttgaag attatataag tcaaaataag ctagtggata aatcaaagaa cactttacca
    31561 acaacagata aaactagaga gaaattttct aaaataaaac ttaatgaaat aaaaaaaata
    31621 tatgaaacaa ttggaataaa tagtattact aaaatatcaa tagataaaaa taagctagag
    31681 atggaaggtg tgtgtagtga tttagatgtt ttaaacaatt taaggaaaat aaagtacata
    31741 aaatattatt acatagacag tattgtaaga tatggagaat attatagatt tagaataaaa
    31801 agttagatgg gtgattattt ttgagattaa ataatagaga aaaaaatcta cttaaaatat
    31861 tgttaatatt agttattata ttggcaagtg atatagcagt aagttatttg gaagaaaacc
    31921 taactagttt gaaaaatgaa aagcaaaata taataaataa aataaataat caagataatt
    31981 atcaaataaa ggataataaa catatagatg ataaatatat agaaagtgtt gaatcaaata
    32041 aaaatgaaga attgaaaaat aattttaata actcattttt agataaaaaa acaaataaag
    32101 atatagtgtt attagtagaa aatgaactga gagatattgt gaatgtagaa tatattaatc
    32161 aagaaagtag tttagataat cacaataaaa aacaagttaa tttagaaata aaagttaatg
    32221 gagaacttgc aaatgtgttg aaacttgaag gtgtaatgaa gaagttaaga ctaaataata
    32281 aacttgaaaa gatagaaatt aataaattaa aagtcaataa ttttgatgtt caaaatattc
    32341 caaatgaata taaacaagaa aaagacaata ttgttgaatg taaaatgaca gttagagtca
    32401 tataacaaaa aagaatagat aaagtaaaaa actattatca attaataaaa ttttatagag
    32461 gaattagttt tacgataatt catttctaca agagggattg ttgatgattt tatcgaaatc
    32521 ctaattaaag ttaatatatt gtaccaaaat ataagtgaat ttaatataat atttacttat
    32581 actatagatt gattaataaa aaaatggtat aataacaaat tgtactttaa tttaataata
    32641 agggagtgga agtcatattg ttagggagaa atgagctatg cccttgtgga agtggcaaaa
    32701 aatacaagaa atgttgttta aataaagata ttgtttcaga aagagcatgc agaaaaactg
    32761 aactttcaca aaaacaatat acagatttat attcaaagtt atatgattat tcaagacagg
    32821 ataagtttaa agaagaatgt gaaaaagcta aagaaatgtt ttatataatg caaaatgata
    32881 gtgtaaatga aaaatttgaa agatttttta atacttattt aatacaagac cacataatgg
    32941 aaaataaaaa ggttatgaca gttggttttc ttgaagaaaa tggtgcaaac ctaagtcaat
    33001 cagaagtatc aatattaaaa agtatgtttg aatcatacgt aagtatttat gaggtaaaag
    33061 aagtttctgc tggaaaaata gtattaaagg actgtttaag tggaaatgaa ctttgtacag
    33121 aagatgtaaa acttctgaga agttttaaaa tcggaagttg tatgatagct agaatagttg
    33181 atattgaagg aactaacata ttgatagata taactataag tatatcaaat gaggttaaag
    33241 atataatttt aaaggatatt atgaatctat tcaatcaata tcaagatgta tacaaagata
    33301 tgaagtcgtt cttaatttat catacacata tactatacaa gtatatacaa cagcttttag
    33361 acccaagtat agctgaatat ttaaagagag aaagagataa aaacgataca aaagttaatt
    33421 cagaaaatga attagttgaa gaagaatgta aagttttaga tactcttaaa gaaaatgtag
    33481 aaaaagaaaa tatagatgca tgtatagagt tttggaatga atataagaaa aatcatgaaa
    33541 atataaaagg agctgaaaat ggttgggcag ctgctgttga atactatgtt aaaaaagaag
    33601 caggacaacc tataactcaa gttcaaatat caaagaaata cgatataagc tctagtacac
    33661 taggaaagag atacaaggat ttaaaaatat cttaattaat tataaaaagc tttaagtcat
    33721 agataaagaa tctatagtat aatatatgta taatgtttag aaagaggtat taataatgag
    33781 tcttagttta ggaaatgcaa ttgctgtatc aacgaacttt ataaaatcta acccagctgt
    33841 acctgtgtac aacggagaag aaatatatgc agttatttat catatagaaa taggatataa
    33901 taatggtgta tcacctgata ccaaacaata tataataata gactataaag agtttgattt
    33961 atctatggat aaaaaaaatg aactaataaa tattgctaaa gagtattgta aatctaaagg
    34021 tattgtagaa aacgaaaacc ttgaaataaa ccttttagac aatgaagaag cagagaactt
    34081 cttagaaaag gtgtttaata atatgaaggt tataagaggt cttataacta aaaaaggata
    34141 aataaatgga ctatatagga atagaaaata taacacctta tgaaaataca tatgaattta
    34201 gtgtatatga atatgatgat gaaatcacct taggtagtga aaagttatat gtatgtgaat
    34261 taagggttgt attgattaaa gttaattctc tgtatgttga aagattgcat aaatcagttg
    34321 aagcaatggt cttagtaaaa aacttgaaaa aagatttaga taaaacactt gttgtaaaca
    34381 aaataaagaa ttttgtgcta gatgagattt gggtagaaaa tctagtaaaa gagaatatag
    34441 aagttatatt tgtagaaagc taggaataac cctagctttt ttatattaca atctaagaga
    34501 ggaaaacaat atgttaagag tatctaatgt aaaacttgga attgataaag atatatcttt
    34561 attaaaagat gttatactta agaagctaaa aataaaagaa aaaaacttag tcaaatatag
    34621 tatatataaa gaatctatag atgctagaaa aaaaggaaaa atggaatttg tttattctgt
    34681 agatgtagaa gttaaagatg aaaataaaat acttaactca aaaataaaag atgtaacaaa
    34741 aataaaggaa atcagatacg taaatgtacc tgtgggtgat aaaaaattaa agaataatcc
    34801 tttggtaatt ggtagtggac cagctggttt gtttgcaggt ctgcttttag ctcaaatggg
    34861 ttatgaacca atcatacttg aaagaggtct tgatgtagac agtagaacca aagatataag
    34921 tgatttttgg acaactagaa aattaaaaaa taattcaaac gttcaatttg gagaaggtgg
    34981 agcaggaact ttttcggatg gtaaacttac aactagaata aaagatatac gatgtagaaa
    35041 agtattagaa gagttagttc agtttggagc accagatgaa atattatact cacataagcc
    35101 acatgttgga acagatatat taaaaaatgt agttaaaaat ataagaaatg aaataataag
    35161 tttaggtggt caagttagat ttaattcaaa agtaacagat atagttgtat caaatgattc
    35221 tatacaatct gtaataataa atgatactga aaaaatagat acagatacaa ttatattagc
    35281 tgttggacat agtgcaagag atacttatga aatgttgtat tcaagaggta taaaaatagt
    35341 tcaaaaacct tttgcaatag gagctagagt tgaacatcca caagatttaa taaataaagc
    35401 acagtacaag gatttttata atcatcctag gttaggagct gcagattata gacttataga
    35461 gcatgtttca aatggaagaa cagcatatac tttttgtatg tgtccaggag gtagtgtcat
    35521 agcatctgca tcaaatgatt ttgaaatagt tacaaatggt atgagtgaac atgcaagaga
    35581 taaaataaat gcaaatagtg catttttagt aaatgtaacg ccagaagatt ttaagagtga
    35641 taatccacta gcaggtgttc atttccagca gaagtatgaa aaattagctt ttgaattagg
    35701 tggaaaaaat tataatgcac cagttcagct tgttggagat tttataaaca ataagacttc
    35761 tttgaattta ggaagtgttg agccatcata tagaccagga tatacactta caaatttaag
    35821 tgaatgtttg cctagcttcg taacagagac tatgagagaa gcgtttatat ctcttgataa
    35881 aaaattgaat gggtttgcaa tgtatgatgc tgttttgacg ggagtagaaa ctaggtcatc
    35941 atcaccaata agaatagtaa gagatgaaaa tactttagaa tcagtgagta ttaagaatct
    36001 atatccatgt ggtgaaggtg ctggatatgc aggtggtata gttacagcag cagttgatgg
    36061 tataaaatgt gcagaaaaaa taatacaaaa atacaccaat aatacaaaaa tttaacataa
    36121 acttaatgat tttttaatat ttaacatgaa tttaacatta tcactcctat aatttgataa
    36181 aatacaatag gttataaaat ttttagatac agtaatctct ttacaaggag gaaataattt
    36241 gaacattata ataagtctaa tgggtggatt aggtctgttc ttatatggta tgaaccttat
    36301 gggagaaggt cttcaaaaat ctgctgggac aaagctaaaa aagataataa aactacttac
    36361 tagtaaccta tttatgggag ttttggtagg tacaggagtt acagcagtaa tacaaagttc
    36421 aagtgctact actgtaatgg ttgttggttt cgtaaatgca ggaataatga ctttaaaaca
    36481 ggcaatagga gttataatgg gtgctaatat agggactact gttacagccc aattggtatc
    36541 tttcgactta actggtatgg ccccagtagc attaggtgta ggtattatac tttatttatt
    36601 tggaaataag cctagaataa aaaatatagc cgaaatactt ataggttttg gtatattatt
    36661 tacaggtatg gattttatga agatggcagt ggagccacta agagactatc aaggatttac
    36721 tgatttactt gttacatttg gtagatatcc actacttgga ttattattgg gatttgggat
    36781 tacagcaata atacaaagtt caagtgcatc tatgggtatg ttggttgcac tggcagcaga
    36841 aggtctagtg ccattatcag cagcactgcc aattctttat ggccaaaata ttggtacatg
    36901 tgtaacttca ctgttatcaa gtattggtgc taacaaaaat gcacgaagag cagctatgat
    36961 gcatctaatc ttcaatgttt taggaacagt gatattctta atattcttga ataaaccagt
    37021 agtaagcatg gttacaagtt gggacccaag caatgttgca agacaaatag ctaatacaca
    37081 tacattgttt aatataatat ctgtattgat acttttacca tttacaaatc ttattataaa
    37141 actagctata aagcttgttc ctgatagagc aggtgatata gatgaagatg aaactaaaac
    37201 tattaaatat atagatgata gaatgataga aactccttct atagctcttg caaatacgat
    37261 aaaagaagca cttcgtatgg gtgagaaggc gaaggaaagc ctaaatgcat ctatggaagc
    37321 tttagtagaa cattcaactg aaaaaataga taagacatat aggcgagaga gattaatcaa
    37381 tgatttacag aaagctattt taaattattt acttaaactt tcaaaagcac ctttagatga
    37441 tgattcaaga gaagttgtgg atactttgtt taatacagtc aatgatatag aaagaattgg
    37501 tgaccacgct aaaaatatag ctgaattatc gcaagttgca atagattcaa atatttcttt
    37561 ctcagaagaa ggacaatctg aacttgatgt tatgtataat agagttgttt cagcatatac
    37621 ttatgcatta gaatcaatga gaactgataa tgttgattta gcttgtaagg ttataaaaat
    37681 tgaagaacaa gttgatataa tggagaaatc ttgtagagct aatcacatgt atagattgaa
    37741 caataatcta tgtagtatag aaaatggagt tatttattta gacgtaataa gtaatttaga
    37801 gagaatatct gaccatgctg tcaatatagc acaacaagtt atagcaaaga gattaggaaa
    37861 tgattaaaaa taattaattt aaaataatat aaaatttaaa atagaggata aataaaagag
    37921 aaactttaaa tgatacgttt agagtttctt tttttgtaaa tttatttgac taaaattttt
    37981 aaatttatac atagttatag catttagaag aatcgatttc ctgataataa atgatgagat
    38041 atgtatgatt ttgtggcaaa cttttaaaaa aaacacctat attttgtgtt ataatctctt
    38101 taaaacgaat atgctgaaaa gagggattta aatgggaaaa ataaattttg attatagtaa
    38161 agctacagag tttttttgtc aaaacgaaat agatgtcatg caaccatatg tagatgttgc
    38221 gcacgacatg ttacataata aaactggttt aggaaatact tttttaggat ggatagacct
    38281 tcctaaaaat tatgataaag aagagtttga cagaataaaa aaatctgctg agaagataaa
    38341 atcagactca gatgttcttt tggtaatagg tataggaggc tcttatttag gttcgagagc
    38401 tgctatagat atggtaagtc attcttttag gaatggactt aaaaaagagc aaagaaaggc
    38461 tccagaagta tattttgtag ggcataatat aagctcaaca tatattatgg atttattaga
    38521 tataattgaa ggaaaggata tttctgtaaa tgtaatatct aaatcaggaa ctactacaga
    38581 accagctctg gcttttagaa tatttaaaga ttacttagag aaaaagtatg gtaaggaaga
    38641 agcaagaaag agaatttatg caacaacaga tgcaagtaaa ggtgcactta gacagttagc
    38701 tacagaagaa ggatatgaga cttttgtaat accagatgat gtaggtggtc gtttttctgt
    38761 attgacagca gttgggttac ttcctattgc tgctgctgga cttgatattg atgctatgat
    38821 gaaaggcgca aatgatgcta gagaagcatt tcaaaatcct gacttaaaaa gtaatgatag
    38881 ttatagatat gctgttgcta gaacaatttt acatagaaaa ggaaaagatg tagagttatt
    38941 agtgaactat gaacctcaac tacattatgt aagtgagtgg tggaaacagc tttatggaga
    39001 aagtgaagga aaagaaaata aaggtttatt ccctgcttct gtagatttct caacagattt
    39061 acattctatg ggtcaataca tacaagatgg aaaaaggctt ttatttgaga cagtacttaa
    39121 tgttgaaaac tgtaagagaa atataactat aagttcagag gaagtagact tagatggttt
    39181 aaactattta gcaggtaaga ctgtagattt tgtaaatcat aaagcttttg aaggaactct
    39241 attggctcat acagatggaa aagtacctaa tttagtaata aatatacctc aactagatga
    39301 gtataatttt ggatatttag tttatttctt tgagaaggct tgtggaataa gtggatattt
    39361 attgggagtt aatccatttg accaaccagg agtagaagct tacaagaaaa atatgtttgc
    39421 tttactaggc aaaccaggtt atgaaaaaga aaaggaagag ctagaaaaaa gacttaaata
    39481 attttaaata gttaaggttt aattaagatt aaaataatat aatgaaatca tcaaaaagga
    39541 gtttactcaa aatagatgat ttcatttttt ttatgaaaaa ttgggaaaaa tatattttga
    39601 taatttatat tatattaaga tagggagtgg tatgagatgt caagaagaaa gaaagggata
    39661 agtttagtaa tattagttgc tataataagt tcaatactta gtagttttct aacaataatt
    39721 ttagttaagg acaatttagt aagtaaatct acaggaagtt ctacgccgat agttgtaaat
    39781 gatgatggta agagtcaaaa tatttatcaa gcagttgcag aaaaagctac gccatctgtt
    39841 gtaggtatta caacaactag tgttgatact agtaatatgt ttgcaatacc aactgaaaca
    39901 caaggtgtag gaactggaat catagttgat tcaaatggat atatacttac taattctcat
    39961 gttatatcag atggtcaagc tacaagtgta aatgtattat tcaatgatgg ttcaacaact
    40021 tcaggtaaag tagtatggtt tgaccaacaa cttgatttag ctatagttaa ggttgataaa
    40081 actggactaa caccagcaga atttgctgat agtgacaaag taaaagttgg agatatatca
    40141 atagcaatag gtaatcctct tggattagat tttcaaaaaa cagttactca agggataata
    40201 agtggattag atagaactat acaaacagaa aaaactaata tgactggttt attacaaact
    40261 gatgcaagta taaatgcagg aaatagtgga ggaccattgt taaatcaaaa aggtcaagta
    40321 attggtataa acacagcaaa agcttctcaa gcagaaggat taggatttgc aataccaatc
    40381 aatacagcta agagtatagt tgaagaagta atcaaaaatg gaaaatatga aaaagtaact
    40441 ttaggcataa aaggtacaga tgtatcaaat tatgaagcag caacaggtac aaaattaagt
    40501 actgataaag gtgtttatgt tgcagaagta atatctggtt catcagcaga aaaagctgga
    40561 gtaaaggtag gggatattat aactaaagta ggagatacag atataacagg tatgaatgat
    40621 ttaaataaaa agttgtatac tttctcaaaa ggtgctagta caaaaataac agtaaataga
    40681 ggcggaaaag cagtaacaat aaatgtgaat ttctaaatat gaccaaaaag atagagacta
    40741 catggtaaag tgtagtctct atttttattt aatttttata gtatatacat ctaaattaat
    40801 taaaatgatg tattgtttat agtttattgc tatattttct tataaaaata aatttaataa
    40861 atatttaaat ttaatttaca aaaacaatat aaatatcata ataaaataaa attttttaat
    40921 attactaaaa atatatagac aaaataataa caaatatagt attatgtaat tgtagaaatg
    40981 cttttcatag gtatattcta tgaagatgaa ttgcataaaa atgaaaaaat ggaatggata
    41041 ttatttaggg gtgaaaaatc atgaatccat tagttataaa cttacaaaaa tgtagcatac
    41101 atgatggacc agggataaga agtacagtat tttttaaagg gtgtccactt gaatgtgttt
    41161 ggtgtcataa tccagagagt caaacatata caaaacaagt tttgtataat gaagaaagat
    41221 gttcaaaatg tgaagcatgt ataaatatat gccctcataa ggcaatttac aaaggtgaaa
    41281 caaaaatatg cttagaccaa gataagtgtg agttttgtga aacttgctta gactattgtg
    41341 taaacaatgc aagagagatt gttggtcaag agtattctgt aagagatttg gttaaagaga
    41401 tagagaagga tagaattttc tatgaagaat caggtggtgg agtaactcta tcaggtggag
    41461 aagtaatggc tcaagatatg gacttcattt gtggagtaat aaacatgtgc aaatcaaagg
    41521 gaatccatgt agcaattgat acatgtggct atgcaaagtc agaaaattat gaaagagttg
    41581 ctaaatgtgc agatttgttt ttatatgata ttaagcttat tgatgaagat aagcacataa
    41641 aatttacagg aaaatcaaat gacttaatac tgaaaaatgt taagatttta agtgaattag
    41701 gggctaacat aaatataaga ataccattaa ttgtaggagt aaatgtagat gatgaaaatt
    41761 tagaggttaa aaaaatgata gagtttttaa agcctttgaa tatacaagca gtaagtcttc
    41821 ttccgtatca taatattggt aaacacaagt atgataaaat ttataaaaag tatgaaggag
    41881 aagaactaca aagaccatca gaagaaaaat tagaggaaat aaaaatactg tttgaagcaa
    41941 gtaatttcaa tactaagatt ggagggtaat aaaatggcaa gaggaacttt tgagagaact
    42001 aaaaaattaa gagaagaaag tattaatgca gagccacaca tatctattga aagagcagta
    42061 cttatgacag aagcttataa aaaatatgag ggaagtgtag agattcctgt acttcgtgct
    42121 ttatcattta agcattatat tgagaataga actcttagca taaatgatgg agaattaata
    42181 gtaggtgaaa aaggtgattc tcctaatgga gcaccaacat atccagaaat ttgttgtcat
    42241 acaatggaag atttagaagt aatgcataat agagatataa taaattttag cgtatcagaa
    42301 gaagctagaa aaatacataa agaagaaata attccttttt ggaaaaagag acaaacaaga
    42361 gataagataa taaatgcaat gacacctgag tggttagcag catatgaagc aggtatgttt
    42421 acagagttta tggaacaaag agccccagga catacagttt gtggagatac aatatataaa
    42481 aaaggatttt tagatttaaa gaaagacata gaagcaagat taaaagagct agattttctt
    42541 aatgatttag atgcatataa taaaaaagca gatttagaag ctatggcaat agcttgtgat
    42601 gcaatggtta tattaggtaa aagatatgct gagaaagcaa gacaaatggc agaagaagaa
    42661 acagatgaag ctaagaaaca agatttatta ctaattgctg aaacttgtga tgttgtacca
    42721 gctcacaaac cagaaactta tcatcaagct atacaaatgt attggtttgt tcatataggg
    42781 gttactactg aacttaatat atgggatgct tttactccag gaagacttga ccaacatcta
    42841 aatcctttct atgaaagaga tgtagaaaat ggaatactag atagagatag agcacaagaa
    42901 ttacttgaat gtttatgggt taaatttaac aatcagccag caccaccaaa agttggtata
    42961 acattaaaag aaagtagcac atatacagat tttgcaaata taaacactgg tggaataaat
    43021 ccagatggtc aagatggtgt taatgaagtt agttatataa tacttgatgt tatggatgaa
    43081 atgaaattga tacaacctag ttcaaatgtt caaataagta aaaagactcc tcaaaaattc
    43141 ttaaagagag cttgtgaaat atcaagaaaa ggatggggtc aaccagcatt ttataatact
    43201 gaagcaatag ttcaagagct tatggaagct ggaaaaacta tagaagatgc aagacttggt
    43261 ggaactagtg gttgtgttga aactggatgt tttggtaaag aagcatatgt tctaactgga
    43321 tatatgaaca ttccaaaaat acttgaatta acattaaata atggatatga cccaattagt
    43381 aaaaaacaaa taggtattga aactggagac cctagaaatt tcaaatctta tgaggaacta
    43441 tttgaagcat ttaaaaagca attacattat atgattgaca taaaaataga aggaaatgct
    43501 gtgatagaaa atatatgtgc aaaacacatg ccttgtccac ttatgtctac aatagttgat
    43561 gattgtatag aaaaaggcaa ggattaccaa agaggtggag caagatacaa cactagatat
    43621 atacaaggtg ttggtatagg aactataaca gatagtttaa cagcaataaa atataatgta
    43681 tttgataaga aaaaattcga tatggataca ttgcttaaag cacttgatgc taattttgaa
    43741 ggatatgaag ctatacttaa tttagtttca aataagactc ctaaatatgg aaatgatgat
    43801 gattatgctg atgaaataat gcaagagata ttcaatgcat attacaatga ggtaactggt
    43861 agaccaactg tttgtggtgg agaatacaga gtagatatgt taccaacaac ttgtcatata
    43921 tactttggag aaattatggg agcaagtcca aatggtagac tttgtgcaaa accagtttca
    43981 gagggaattt cacctgaaaa aggtggagat actaatggac ctacagcagt tataaaatct
    44041 tgtgctaaga tggaccacat aaaaactggt ggaacattat taaatcaaag atttgcacca
    44101 tcagtagttc aaggagaaaa agggttagat aatatggcta atctagtaag agcatacttt
    44161 aatatggatg gtcatcatat acaatttaat gtatttgata aaaatgtact gttagaggct
    44221 caaaagaatc ctcaggatta taaagactta atagttagag ttgcaggata tagtgaccat
    44281 ttcaataatt taagtagaat actacaagat gagataatag gaagaactga gcaaacattc
    44341 taggtttgct tgtaaataaa acttcttact atataaaatc gtaactgata taataaagta
    44401 taaatggttt gattccattt ttaaaatttt agattaatta tgagctaact ttctaaatag
    44461 gaagttagct cgttaagttt aatgaaaaaa tcgttaaatt tatatttaat aaatcataca
    44521 catattagta agtaatacat acaaaagaat aatttattat agttataata aggttatatt
    44581 ttataaaaat aagataattt taagcagtta aaataaagtc aatttagcta ctttaaaata
    44641 aaactaatag aaaaaaggag atttgatatg aaaactttag gatttattgg ttcagggaat
    44701 atgggaagtg caatgatagg tggcatagta cagtcaggat tagtaaaggc agaagatata
    44761 actgtatcag atttaagcca agttgcctta gataaagtaa aagaagaatt tggagtaaat
    44821 actacaactg attcaaaggt agctgttaca aattctgatg tggtaatagt agcacttaaa
    44881 cctaatatat gcgaaaaggc tttaactcca cttaaagaat taatagatgc aaataaaata
    44941 atagtatcta tagcagcagg taaaactata gaaagtctgg aaagttttat aggttcagat
    45001 aaaaaaatag taagagtaat gcctaatact ccagcccttg ttggcgaagg aatgtcagct
    45061 ctttgtaaaa atagtaatgt aacagatgaa gagttaaata tgattaaggc tctgtttgaa
    45121 tcttttggtg aggcagaaat agtatcagaa tatttaatgg atacagttac aggtattagt
    45181 ggttcttctc ctgcatatgt atttatgttt atagaagcta tggcagatgc agcagtatta
    45241 gctggaatgc caagacaaca agcttataaa tttgcatctc aagcagtaat ggggtcagct
    45301 aagatggttc ttgaaactgg aaaacatcca ggtgaattaa aagatatggt ttgttctcca
    45361 gcgggaacta ctatagaagc tgttaaggta ttagaagaag aaggatttag agcagcagtt
    45421 ataaaatcta ttgttgcttg tatagataag tctaaagata tgagtaaata acagatgtaa
    45481 ttaagtttac tatatggttt cttgaattgt ttttttagtg cttatttata tctataaata
    45541 aatgtatttg gtataagtaa aataagagta atttac
//
LOCUS       DACXMZ010000006        44253 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.006, whole genome shotgun sequence.
ACCESSION   DACXMZ010000006 DACXMZ010000000
VERSION     DACXMZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.006"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(114..692)
                     /locus_tag="JD016_01310"
     CDS             complement(114..692)
                     /locus_tag="JD016_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAU4998265.1"
                     /translation="MISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFLKIDLSDIPA
                     IFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMILCYSYKRSGD
                     LKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINPQVHDLFTFV
                     IWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(844..961)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1021..2187)
                     /locus_tag="JD016_01315"
     CDS             complement(1021..2187)
                     /locus_tag="JD016_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAU4998266.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEIGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(2335..4476)
                     /locus_tag="JD016_01320"
     CDS             complement(2335..4476)
                     /locus_tag="JD016_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAU4998267.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(4620..5636)
                     /locus_tag="JD016_01325"
     CDS             complement(4620..5636)
                     /locus_tag="JD016_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAU4998268.1"
                     /translation="MKNIQINEEKMNQQDFWNDNEVAQRVLQENKSLKETLEEYESLK
                     SLLEDIEVLIEIGLEEDDDSVERDIEKSIESMEEKLSEMKIKTLLNGEYDKNNAILSI
                     NAGTGGLDAQDWAQMLLRMYIRWSESKGYKVKLLDIISDPEAGIKTATILVEGTNAYG
                     YLKSEKGVHRLVRISPFDPSGKRHTSFASIDVTPELDENIEVEINPSDLKIDTYRASG
                     AGGQHVNTTDSAVRITHIPTGVVVQCQNERSQHLNKDRAMRLLMAKLIELKELEQKEK
                     IEDIQGKYSQITWGSQIRSYVFQPYKLVKDHRTNAEFGNVDSVMNGNIDLFINEYLKM
                     NKIV"
     gene            complement(5742..8417)
                     /gene="secA"
                     /locus_tag="JD016_01330"
     CDS             complement(5742..8417)
                     /gene="secA"
                     /locus_tag="JD016_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAU4998269.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(8549..9100)
                     /gene="raiA"
                     /locus_tag="JD016_01335"
     CDS             complement(8549..9100)
                     /gene="raiA"
                     /locus_tag="JD016_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAU4998270.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(9652..10326)
                     /locus_tag="JD016_01340"
     CDS             complement(9652..10326)
                     /locus_tag="JD016_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAU4998271.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKESIVHNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(10329..11381)
                     /locus_tag="JD016_01345"
     CDS             complement(10329..11381)
                     /locus_tag="JD016_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAU4998272.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSVLYGLYAIKELNLKLDKSVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KFQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(11430..12389)
                     /locus_tag="JD016_01350"
     CDS             complement(11430..12389)
                     /locus_tag="JD016_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HAU4998273.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(12485..13003)
                     /locus_tag="JD016_01355"
     CDS             complement(12485..13003)
                     /locus_tag="JD016_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HAU4998274.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGIMMDPFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            complement(13014..14348)
                     /gene="celB"
                     /locus_tag="JD016_01360"
     CDS             complement(13014..14348)
                     /gene="celB"
                     /locus_tag="JD016_01360"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAU4998275.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGNVHEFIFNFL
                     QIPLLKLGNTLSATAIAYVFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGT
                     KLPNIITAQFQDMFATFGGAGSTLSLIIAMLLICKSKRIKSIGKLSILPGIFGINEPL
                     IFDLPIMLNPLLLIPFALVPTVNIIIAYFCMSTGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKILDKQYLKDEKSNVAEEDDDISFDDLVL"
     gene            complement(14387..14713)
                     /locus_tag="JD016_01365"
     CDS             complement(14387..14713)
                     /locus_tag="JD016_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU4998276.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(14737..15045)
                     /locus_tag="JD016_01370"
     CDS             complement(14737..15045)
                     /locus_tag="JD016_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAU4998277.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(15186..17099)
                     /locus_tag="JD016_01375"
     CDS             complement(15186..17099)
                     /locus_tag="JD016_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAU4998278.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(17431..18213)
                     /locus_tag="JD016_01380"
     CDS             complement(17431..18213)
                     /locus_tag="JD016_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAU4998279.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(18216..20435)
                     /locus_tag="JD016_01385"
     CDS             complement(18216..20435)
                     /locus_tag="JD016_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAU4998280.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(20699..21796)
                     /locus_tag="JD016_01390"
     CDS             complement(20699..21796)
                     /locus_tag="JD016_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998281.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPVIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(22112..23305)
                     /locus_tag="JD016_01395"
     CDS             complement(22112..23305)
                     /locus_tag="JD016_01395"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAU4998282.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(23442..23545)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            23716..24243
                     /gene="yyaC"
                     /locus_tag="JD016_01400"
     CDS             23716..24243
                     /gene="yyaC"
                     /locus_tag="JD016_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HAU4998283.1"
                     /translation="MKGVFLLYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDR
                     AIGDTLGPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDAC
                     LGSQSNIGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFI
                     LDLAETIALALLVST"
     gene            complement(24354..24782)
                     /gene="fabZ"
                     /locus_tag="JD016_01405"
     CDS             complement(24354..24782)
                     /gene="fabZ"
                     /locus_tag="JD016_01405"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAU4998284.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(24798..25817)
                     /locus_tag="JD016_01410"
     CDS             complement(24798..25817)
                     /locus_tag="JD016_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAU4998285.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(26014..26274)
                     /gene="spoIIID"
                     /locus_tag="JD016_01415"
     CDS             complement(26014..26274)
                     /gene="spoIIID"
                     /locus_tag="JD016_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HAU4998286.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(26372..27040)
                     /locus_tag="JD016_01420"
     CDS             complement(26372..27040)
                     /locus_tag="JD016_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HAU4998287.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(27106..28170)
                     /gene="spoIID"
                     /locus_tag="JD016_01425"
     CDS             complement(27106..28170)
                     /gene="spoIID"
                     /locus_tag="JD016_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HAU4998288.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(28257..29528)
                     /gene="murA"
                     /locus_tag="JD016_01430"
     CDS             complement(28257..29528)
                     /gene="murA"
                     /locus_tag="JD016_01430"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAU4998289.1"
                     /translation="MRRSKNMAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGK
                     STLNGVPNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFL
                     VMGPLLARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDYGFVEAATEKLVGN
                     KLYLDFPSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNT
                     IKIKGVKELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAG
                     CEITEMDNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFE
                     NRFMHVAEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGET
                     QIGEIYHIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(29531..30268)
                     /locus_tag="JD016_01435"
     CDS             complement(29531..30268)
                     /locus_tag="JD016_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998290.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(30696..31991)
                     /locus_tag="JD016_01440"
     CDS             complement(30696..31991)
                     /locus_tag="JD016_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HAU4998291.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(32443..34275)
                     /gene="glmS"
                     /locus_tag="JD016_01445"
     CDS             complement(32443..34275)
                     /gene="glmS"
                     /locus_tag="JD016_01445"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAU4998292.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(34542..35888)
                     /locus_tag="JD016_01450"
     CDS             complement(34542..35888)
                     /locus_tag="JD016_01450"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAU4998293.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAILEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(36058..36615)
                     /locus_tag="JD016_01455"
     CDS             complement(36058..36615)
                     /locus_tag="JD016_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HAU4998294.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(36616..37368)
                     /locus_tag="JD016_01460"
     CDS             complement(36616..37368)
                     /locus_tag="JD016_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HAU4998295.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(37368..38447)
                     /locus_tag="JD016_01465"
     CDS             complement(37368..38447)
                     /locus_tag="JD016_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HAU4998296.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(38476..38691)
                     /locus_tag="JD016_01470"
     CDS             complement(38476..38691)
                     /locus_tag="JD016_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAU4998297.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(38734..39411)
                     /locus_tag="JD016_01475"
     CDS             complement(38734..39411)
                     /locus_tag="JD016_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAU4998298.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(39401..40480)
                     /gene="buk"
                     /locus_tag="JD016_01480"
     CDS             complement(39401..40480)
                     /gene="buk"
                     /locus_tag="JD016_01480"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HAU4998299.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(40502..41404)
                     /gene="ptb"
                     /locus_tag="JD016_01485"
     CDS             complement(40502..41404)
                     /gene="ptb"
                     /locus_tag="JD016_01485"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HAU4998300.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(41592..41711)
                     /locus_tag="JD016_01490"
     CDS             complement(41592..41711)
                     /locus_tag="JD016_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998301.1"
                     /translation="MNKYFEYLVVTRCDKKTYKIKFVNFMFLLHLVYFSYIIK"
     gene            complement(41708..42889)
                     /locus_tag="JD016_01495"
     CDS             complement(41708..42889)
                     /locus_tag="JD016_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998302.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(42886..43725)
                     /locus_tag="JD016_01500"
     CDS             complement(42886..43725)
                     /locus_tag="JD016_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAU4998303.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
BASE COUNT        13178 a         8426 c         4724 g        17925 t
ORIGIN      
        1 ttgataaatt aataaagcca taatatattt aatatatcaa tcacaatata ggactgaagt
       61 tataaataaa ttatggctat tcaatacatt taattttata ttttcttgtc taattatttc
      121 tttattatat ttcccatttt tttataaagt ggcaatgtta ctaaagatat tataacagcc
      181 ttcagcacat taaatggagc tatcatccaa attacaaaag tgaataaatc atgaacttga
      241 ggatttatag ctgaacctaa acctattata gcatctagtc ccataagttg accataaagt
      301 ggcaacatta caaaataatt tactaatgaa ccaattatag tcatagatat tgttcctaat
      361 acaaaaccaa tcagtacacc ttttaaatct ccacttctct tatatgaata acaaagtatc
      421 attacataag aaccaccaat taatatattc gcaaattctc ctattcctcc agttgttgtt
      481 gctgttattg cttgtaacac attttttact acaactattc caaatccaac taatggtcct
      541 agagacattc ctccgaatat tgctggaata tctgataagt caatttttaa gaaatctgga
      601 aatatacctg gtattggtgc ttgaataaac attaataaat aagatattgc agataaaatt
      661 gacattacta ctaaagttct tgttgaaatc atattgcctc tttttgctgt gttttgcata
      721 attattttcc ccctaatcaa tatgtttata ttttttagga aaaacaaaaa actcgaagac
      781 cctatcttcg agtttttatg gatatagttt aaacaaacta aaataaactt atttatcttc
      841 cataaatatc ttctctcatc cagactttac tgtcggcttt ggaatctcac caaatcaacc
      901 acctaagtag ttcgcgggct ataccgccgg tagggaattt caccctgccc cgaagatctt
      961 ctttgatttt ttaattttcc ttacactctt atattagtta attttttaat aattgtcaag
     1021 ttattttttc tcatacacta ctttgccatt tattatagtg taaagtacat tactttgaat
     1081 ctctagtgga gaattatccc aaattactag gtctgcatct ttaccaattt ctatagagcc
     1141 tactctatct tctattccta aagccttagc tggatttatc gtaattgatt ctattgcttt
     1201 ttcttctttc ataccatgtt ttactgcaat acctgcacat attggaagat attgaactgg
     1261 tattactgga tggtctgtca ttatacatac atcaagacct gcatttgaaa gtattcctgc
     1321 tgtattaaat gtcaaatttc taagctcaaa tttagacctt tctgacaaag atggccctac
     1381 tattacagga aacccttctt ccactaattc atctacaata agatgacctt ctgtacaatg
     1441 gtctaatgta agctttaaat taaattcttt tgcaattctt attgctgtaa acatatcatc
     1501 tgctctatga gcatgtgcct taaaaggaat ttctcttctt aaaactggta ttaaactttc
     1561 catttttata tcatattcag gtttttcaca gtcttcatca tcatgactct catacatatc
     1621 aatatcctct aaatattctt ctgctttttt taggttttct ctaagtaaag cagctatagc
     1681 cattctagtt tgaggagatt tatcatcctg accataacag ctctttggat tttctccaaa
     1741 agcaatttta gatgcaactg gattttttat taccatttta tcaattcttt ttccacatgt
     1801 ctttatagct atacattgac cacccataac atttgcactt ccaggggttg tacaaacaga
     1861 agttattcca ccttcaaaag cttctttaaa agttctatcc attggattaa ttccatctat
     1921 aggatttaat tgtggtgtta ttgggtcagt ctcttcattt ccatctgctc cttcaaaccc
     1981 tataccatct tcccataaac ctaagtgagt atgagcatct ataaatccag gaaacacaag
     2041 tttattacta gcatctatta cctctacatc taaaggagct atcaaatctt ctccaatttc
     2101 tattattttt ccttcatcta taagtatatc tccatgaatt atcccatttg ttatggtatt
     2161 tattttacca ttttttataa aaatcattca ttttcctcct gattttcaag actatcgttc
     2221 ttattttaat atattttctt actatatact tttacaaatt tttaacattt atacactcga
     2281 gtaatctcat ttacaaatat agaatttaag tactataacc atacataaac ttatctattt
     2341 tttcattgat aaggcaactc tttttttgtt taagtcaatt ccaatgactt ttacatctac
     2401 tatatcacct actgtaacaa tactcatagg gtctttcaca aaactattac tcatttctga
     2461 tttatgaact aatccgtcat tttttattcc aatatctaca aatgcaccaa agtctacaac
     2521 atttcttatt gtacctttta aagtcatacc ctcttgtata tcttcaattt ttaagacatc
     2581 agttctaagt ataggtttta taccttcttc tcttgggtct cttcctggtt ttttgatttc
     2641 agctatgata tcttttaacg taacttgtcc tacttccagt ttttcactta attccctcaa
     2701 gccaatttct tttatttttt catctatttc acctatattc ttattcttta catcatctaa
     2761 agaatatcca attatctcaa tcattttttt acatatatca taactttcag gatgcacacc
     2821 tgtattatct agtggatttt taccctctaa gattctcata aatccagcac attgagtaaa
     2881 tgcttgaggt cctagtcttt ttactttttt caattgtgct cttgacgtaa aatctccatt
     2941 ttcttctcta tatgctatta tattttttgc tatagcttta cttattcctg caacatgttc
     3001 tagtagggag tatgatgcag tatttaaatc tactccaaca ctatttacag aatcttcaac
     3061 aactccatct agtacttctt ctaatctctt tttgtttaaa tcatgctgat attgaccaac
     3121 accaatactt ttagggtcta tttttacaag ctctgctaat gggtcttgta atcttctagc
     3181 tatagatata gctcctctta ttgatacatt tatatctgga tgttcctcat ttgcaagttc
     3241 agatgctgaa taaacagatg ctcctgcttc actaactatc acatactgca cttcgctatc
     3301 tatttctttt atcatttctg atacaaaagt ttctgattct ctagatgcag taccatttcc
     3361 aattgaaatt atatctatat cgtatttttc aatcaatcct tttaaaactt tctttgctcc
     3421 ctctacatca ttttgagggt ctgttggata tactgttgta taatctagta atttaccatt
     3481 tttatctact acagctattt tacatccagt tctaaatgca ggatcaaatc ccataactac
     3541 tttgtcttta actggtggtt gaagcaataa acttttaaca ttctttccaa atacacttat
     3601 agctctttct tgagctattt ctgttaaatg atttcttatt tctctttcta ttgatgggaa
     3661 aattaatctt ttataagaat cttctattga acttacgatt tcttctttat ttttgaaatt
     3721 tttatcattt acatattcgt ttattatgta atttaaaact ttatcattat ttatttccaa
     3781 cttaaccttt aggaagcttt ctttttctcc tctgtttatt gccaaaactc tatgtggagc
     3841 catacttttt actgcttcgc tataatcata atacatatca taaaccgatt tctcatcagt
     3901 agcacttttg ctaactatca taccttctct taaagctaat tctcttatat actttctaat
     3961 cttagcatca tctgagacta gttctgcaat tatatctcta gcacctttta gagcatcttc
     4021 aatagaaaga acttctttct cttcatcaag ataattcttt gcctctattt ctatattatc
     4081 taaattattc tctagtattg acaatgctaa attttctaat cctttttctt ttgctattgt
     4141 tgctctagtt cttttctttt gtttatatgg agcatatata tcttctactt cttgtaatgt
     4201 ttttgctttt tctatatttt gtgttatttc atctgttaat ttcccttgtt catctattag
     4261 tctaactaca tcatcttttc tactttgaag atttctcaaa tacattaatt tttcataaaa
     4321 ttctcttagt gtaacatcac tcatttcgcc agtcatttct tttctatatc tagctataaa
     4381 tggtattgta tttccttcat caataagttt taatgtatta tttatttgtt catctctaag
     4441 attaaactct ttttttaaga tttgatttat atccaattta ttcactccta atgttagttt
     4501 ttcataagta taacaaaaca gaacgaaggc tgagggttat cgtcctcagc ctaagtgctg
     4561 taagtgcata ttaagcacta accttcttat acatatatct taattacagt gcatatttat
     4621 taaactattt tattcatttt taagtattca ttaataaaca agtctatatt tccattcatt
     4681 acactatcta catttccaaa ctcagcattt gttctatggt ccttaaccaa tttatatggt
     4741 tgaaaaacat atgacctaat ttgactaccc caagttattt gtgagtattt gccttgtata
     4801 tcttctattt tttccttttg ttctaactct tttaattcta tcaattttgc cattaaaagt
     4861 ctcatagctc tgtccttatt tagatgctga gacctttcat tttggcactg aactactaca
     4921 ccagtaggga tatgtgttat tctaactgct gaatcagtcg tatttacatg ttgccctcca
     4981 gctccagaag ctctatatgt gtctatcttt aaatctgatg gattaatttc tacttctata
     5041 ttttcatcta actctggtgt tacatctata gatgcaaatg aagtatgtct ttttcctgat
     5101 gggtcaaatg gagatattct cactaatcta tgaactcctt tttcactctt taaataccca
     5161 taagcatttg ttccttctac aagaattgta gctgtcttta tccctgcttc agggtctgat
     5221 attatatcta aaagcttaac tttataaccc ttactttctg accatcttat atacattctc
     5281 aaaagcattt gagcccaatc ttgagcatct aagccaccag taccagcatt tatagataat
     5341 atagcattat ttttatcata ctctccattt agaagagtct ttattttcat ttcagataat
     5401 ttttcttcca ttgattctat ggacttttct atatctcttt ctacagaatc atcatcttct
     5461 tctaaaccta tttcaattaa tacttctata tcttctaata agctttttaa gctttcatat
     5521 tcttctaatg tctcttttaa agatttattt tcttgaagta ctctttgagc gacttcatta
     5581 tcattccaaa agtcttgttg attcatcttt tcttcattaa tttgtatatt tttcatcaat
     5641 gaagctatgt caaagagaaa ccctcaattc ttctaaattt gaagccatct gctccatact
     5701 atgtctatac tcttgtaact ttaacattta atcctccgag attatcttcc acaacagttt
     5761 ttatattttt taccacttcc acaagggcat aagtcatttc ttccaacctt ttcatctttt
     5821 ttaacagttt tacttgttgg aagtgttcca tctgatggag aagatagatt ttcaacatct
     5881 acaactgctt ttctttccac tggagtttct attgttatat taaataaata tcttacagta
     5941 tcttctttga tatgtttatt catttcatca aacatatcaa aaccttccat tttataagca
     6001 ataactgggt cttgctgacc tacagcacga agaccaatac cttggcgtaa ttggtccata
     6061 gcatctatat ggtctatcca atggttatca acagcttgaa gtaagattac tctctcaact
     6121 tctctcattc tctcatagcc aacttgttct tcctttgaag tatatatctt catagctatt
     6181 tcataaactt tttctgttat ttcaactgga ttaagttttt ctatttctgg tatttctatg
     6241 ctaccttttg gcaagaacaa attatacata tgttctttga atccttcttc atcaaatcct
     6301 ttatcttgtg tataaagagt aactgcttct tctattatag aatgagtcat tgattgtatt
     6361 tgttcttgta aatcttcacc ttctaataca cgttttctct ctgcatatat tatttctctt
     6421 tgtttattca taacatcatc atattgaagt acatgctttc ttattccaaa gtttttacct
     6481 tcgactttct tttgtgctcc ttcaatagac ttagataata ttctatgttc tatcggcatg
     6541 tcttcttcaa gacctatttt atctacaata ccagatattc tatcactacc aaacaatctc
     6601 ataaggtcat catcaagacc tatatagaat cttgaactcc ctgggtcacc ttgacgtcca
     6661 gcacgacctc tcaactggtt atctattctt cgagattcgt gtctttctgt accgattata
     6721 gcaagtccac ctgcttctac aacttgtttt tgttcttctt gtgtttgttg cttaaacttt
     6781 tcaaatagtt tttcatactc ttctctagct tcaaatagaa tttcattacc ttttacaggt
     6841 ataccttcta ttggagtatc tactctattt actatttctt ctttaaatcc atttcttctc
     6901 atttctctct tagttaaaaa gtctggattt ccacctagaa ctatatctgt accacgacca
     6961 gccatattag tagctattgt aacagctcca agtctaccag cttgtgctat tatttctgcc
     7021 tctttatcat gatgcttagc atttaatacc tcgtgcttta tgcccttttt ctttagtatt
     7081 tgtgataata attctgattt ttctattgat actgtaccta caagtattgg ttgattgact
     7141 ttgtgccttt caattatttc ttgtgctaca gcattaaatt tacctatttc acttttgtat
     7201 acacaatctg gtaagtcttc ccttatcata agcttattag taggtacttg gaaaacatcc
     7261 attttgtaga ttgctttaaa ctcttcttct tctgtttttg ctgtaccagt cataccagaa
     7321 agttttttgt acattctaaa gtagttttgg aatgttacag tagctagtgt tttagattct
     7381 ctttgtattt taagaccttc tttagcctct attgcttgat gtaaaccttc tgaatatctt
     7441 ctaccaaaca taagtctacc tgtaaactca tctactatta ctatttctcc atctttagca
     7501 acataatcaa catctttctt cattattaca tgtgctctta aagcttgatt tatatgatgg
     7561 tataattctg tatgagatat atctgttata ttatcaacat taaaatacac ttctgctttt
     7621 tgtatacccg atgctgttag agatacagcc ttatcttttt cttctaattc ataatcatct
     7681 ggttttaagg ttaaaacaaa tgtgtttgca tctgaatata agtgagttga tttgtctcca
     7741 ggtccagata tgataagtgg tgttctagcc tcatctatta gtatagagtc aacctcatcc
     7801 actatagcat aatttaatcc tctttgaact ctttgctctt tgtgtattac catattgtcc
     7861 tttagatagt caaatccata ttcattattt gtaccataag ttatatcaca atcatattgt
     7921 tcttttctaa cttttggatt ttgaccgtgt ataataacgc ctacactcat tcctaaaaac
     7981 tcatagattt ttgccatttg gtctctatca cgttttgcaa ggtaatcatt tactgttaca
     8041 acatgtacac cttttccagt aagcgcattc aaataaacag gagcagttgc aaccaaagtt
     8101 ttaccttcac ctgttttcat ttctgctatt cttccttgat gtagtactat acctccaatc
     8161 atttgaactc tgtaatgtct aagtcctaac actctcttag aaacttctct aactactgca
     8221 aaagcttctg gaagtatatc atctatactt tcaccatttg ctaatctttc tttaaatata
     8281 tttgtcatat ttttaagttc cgagtctgcc atagattcaa atttaggttc taatgaatct
     8341 attatatcaa cagttttatt gaactttttt aattcttttt tatctgccat gttgaataaa
     8401 ttatccataa agctcatatt ttacatctct cctcgttctt tattgtacta ttattcattt
     8461 taccataaaa actatacttt taatattaat ttaaaaagct atcgataatt tatttatcga
     8521 tagctaatat gattaaatct aaattgcttt attcatgttc tattatacca tatccaccat
     8581 tattacgctt ataaactact gctatttcat ctgaatctat gttcttgaac atataaaaat
     8641 catgtcctat taattccatc tgtaatacag cttcttcttc actcattggc ttaacactaa
     8701 atttcttacg tctttgaatt actaattctt gattttcatc cccataatcc tcttgttcat
     8761 cagctagatt tatctctgca tcttggaatc ttatactctt gttgtcttta tgtttatctt
     8821 gtaatctctt tttatatttt tttaattgtt tacttaattt atcatataca acatctatag
     8881 ctgtgtataa attttcctgc atatcttctg ctctaattat aggaccattt acagtagcta
     8941 ttgttacttc tattttttgt cttgcttttc tcgcacttac tgtaactttt acatcagtgt
     9001 ctggatgaat gtaattatct aacttactta gtttagtttc gattttattt tttattgcat
     9061 cagttaattc gatttgtttt ccagatataa ttatgttcat aaatacatct cccttcaatt
     9121 atgccagtat aagaaaattt atactttaat aatatattct acacgaactc tcataatcct
     9181 ctttttatta tttactttta tgattttttc tattcaattg atagaattat ttatcatttt
     9241 tagttacatt gatacttatg tggattaata ttattacaaa cacctgtgga taatgtggat
     9301 aaaactgtta ataactcttg tttttgttta tttaaagcta tttttactcc tatttctatc
     9361 cacaaaaatc tccattaatt gtttattaat atgtgaattc atgtcataag atggtaattt
     9421 aggaaattat gtgtattata tgccaatata cctttataat tttttaataa atataaaccc
     9481 tctaaaacct ccataatcta tcttttcttt cattatttat atttaaaact acttaaaagt
     9541 tataaagcat actcaacaat aatttcatat ttaataccta ttaacattca tatacccaaa
     9601 ttataattaa cttactctct ttatttaaac taagtatttt atattcatat actatcttga
     9661 aattatattt acgagttgtt taagcttttg tctatctata tcttcaaaag gactgttatt
     9721 tattctaatc gctgtaccaa aatgaaaatt acttgcctta gttaattcct ttatcttttc
     9781 tatattatta aaattaagac ctccacctaa aagaattttt atgtgattag aactaagcat
     9841 catatttttt atcatttgta tgttatgaac tatactttct ttcccacctg atgttaatat
     9901 attcgtaatt ttatcataat ccttcaaaat tttaacacta tctattgtat ttgattcatc
     9961 tatggctcta tgaaatgtaa catcaagatt atcacaacat ttcaataaaa catttagatt
    10021 tttctcatct atattgttgt ttttatccaa tacaccaaat acaactccat tagcaccaat
    10081 ctctttaacc acacttatat ctttttgcat tatacttata tcttctttac tgtatacaaa
    10141 acttttcgca tgatgtctta tcattacgtt cacaggtatc tttacactgt taacaacact
    10201 ttctataaga ccaaaacttg gtgtcaatcc accttcagtt aaagcactaa ctaactctat
    10261 tctatctgca ccacaatctt ctataatttt agcgtcttct actgacattc ctattatttc
    10321 taacataact actcctttgc taattcatat attgcatttg caaatatctt tgcatttaat
    10381 attatatctt ctatatccat gtattcatca ggattatgag ctattccttt ttgtcctgga
    10441 aatgaaggtc caaacggaac tatatttggc attacttttg catacgtacc tccattagtt
    10501 gttactggag ttccatctaa tgatgtaacc ctttcataag ctagctgaaa ctttttaaca
    10561 agattactct ctttatcaaa atatacagga ttaaaattag aaattacctc tatatcgata
    10621 ctatttggaa aactagcttt tatttcatct aatacatctt tcaatttata actaaaatcg
    10681 tattttacct ttatatctaa tatagtatca ctattttcta tattcagctc tttatatgta
    10741 acaagcacat tttcagacat attttctata aaacctaggt atacatcttc ttcataattg
    10801 attttacttt ttattctaat ctttgccata cctttttcac catatacaac aggatattta
    10861 caatctggtg taaatcccat aataggtgct ttttcttttt ctaaataata tggtatatct
    10921 tcaaatccac tttcttcatt tgtaccaaat attattctaa cgctcttatc taattttaag
    10981 tttaattctt taatagcata taatccatat aagacagaca ttataggtcc tttattgtct
    11041 agtactcctc gtccataaat tcttccatta gtttcttctc ccttataagg ctgatgcttc
    11101 caaccatctc cttcatgtac aacatctaca tgtccaatta cacaaacata atcatcacta
    11161 tctccatgct ctgcataacc aatataatta tccaagtttc taaccttaaa tcccaatttt
    11221 tctgatattt ctaatgcttt attaagtgcc tcccctactt tttcgccaaa agggaagcca
    11281 ttttctgact cccctttgac actaggtatt tttacaatat ctataatatc acttaataaa
    11341 tcttctttta actcatctat tttattactt atgatattca taaaaatctc cttattataa
    11401 aactctattt taattatgta accactactt tactcactta cttctattgg tgcttttatt
    11461 ctagcctcat atgcttccat ccactcattt gatataacct tatgtatagt ctttccatct
    11521 actctatttg aaatatatac agtaggagat tcttctttag ttactactga aaaagtaaaa
    11581 tcagctatat ttgtagctac tccccattca cctttattat tcatggcaac taatgataaa
    11641 tcaccagctt ttcctcttct tttgataagc tgtttatcaa gttcatttac tgcaatatca
    11701 catgcttctt gtggatgctt cccttctttc ataagtctaa ctatctcata agatatacat
    11761 cccttcatta aatcttctcc caaaccagta gctgtagctc ctcctatact actatcaaca
    11821 tagaatcctg aaccagatag tgcagaatct cctattctac ctcttttctt cataaacaat
    11881 ccacttgttg atgtagctgc acacattttt ccagttttgt ctaaagatat cataccaaca
    11941 gtatcatgac cagcatatgg gcttaaccct ttgtctaaag tctctttttt tctcttttta
    12001 taatgtaact tcgctctatc agtcaacata ttttttcttt caaatccatt tttatgagca
    12061 taatcttcag cacctattcc aactaaaaaa ttatttacct tctcataact taacttccta
    12121 gctatgctga caggattaga ataatctctt ataccagcaa ctgctccaat atctaatgtg
    12181 ttaccatcca taaatgcagc gtcaagttct acctcacaat tttcatttgg aagtccacca
    12241 tatccaaccg atttatagaa tggatagtct tcaactcttc ttacagcaag ctctacagca
    12301 tcacctgctt ccatgccttt ttctaaatgt tctgatgcct cagttattcc ttctagggcc
    12361 attctccatg tcgctatcat tccccacatg ttaaaattcc cctccttaaa ttatatagat
    12421 tttactaatg tatgcttatt ataaatagta taatatttaa acttgctaat aattaatact
    12481 cttattaata acaaaattct tctttcatca aagttatatc ttctttattt gctattgctt
    12541 ttgccaattt acatatgtta tttaatgaat cattaagacc aattccacct ttttttggag
    12601 tcttaactat acatatgtca tttttatatt cgtttatcac atctatattt tcttctgaca
    12661 tagctgcaaa agctggtata tctgttttat catttatatt tttagacagt attgctgaca
    12721 tcttagaaaa gttttcgtaa ttaaagcttg gtccacagat tactacatca gggtttaatt
    12781 tttttaccat agctatcatc ttactcgtta cttcttcttg attatttaca aaaaattcat
    12841 ccccacaaaa taaggtagct attaccttag attcatttaa aaatggatcc atcattatac
    12901 ctggtcctaa tgggcttgat tttccagcag gaggtatatt acttttctct tttcctccag
    12961 ctccagcttg tatttggtcc aatatcatca ctattttctt cataatcttc ctcctataat
    13021 actaaatcat caaaagatat atcatcatct tcctctgcaa catttgattt ttcgtctttt
    13081 aaatattgtt tgtctagtat ttttataaat ggtatataca taaatattcc tagtataaga
    13141 agtactaatt gtaaaactgc cccttgccat cccgttgtta gaaatccaga gaatataatt
    13201 ggagttgtcc acggtagctg aactccattt gtaagtggta ctaatcctgt tgacatacag
    13261 aaataagcta ttattatatt tactgttgga actaatgcaa atggtatcaa tagcaatgga
    13321 ttaagcatta tcggtagatc aaatatcaat ggctcattta taccaaatat acctggtaaa
    13381 attgataatt ttcctattga ttttatacgt ttacttttac atattaataa catagctata
    13441 attaatgata atgtactacc tgccccacca aatgttgcaa acatatcttg aaattgagca
    13501 gttattatgt ttggtagttt tgtaccagct tgaaaagcag ctaagttttc agctgataga
    13561 atttttaata ttggattata cacagctcca acaacactac ttccatttat tccaaagaac
    13621 cagaatagat gtaagaatac ataagcaatt gctgtagcgc ttaaagtatt tcctaatttt
    13681 aataatggta tttgtaaaaa attaaatata aattcatgta catttccata tggtgtagct
    13741 tcaaaagcta tcttaactaa aaagaaaact accataacta ttgcacttgg tattaatgct
    13801 gcaaatgatt ttgatacggt tggaggaact ccatctggca ttttcaatgt ccatcctttt
    13861 tttacaactg cagcaaataa ttttacagag gttattgctg ttatcatacc tacaaacatt
    13921 cccttagagc ccatccaacc tagtggtata cctgttacca tgtaaactga ttctgttcct
    13981 tctggtgtat atggtattac aaatggtgtt attataaaaa aggcaactat tgcaactgct
    14041 gctgatgcta ttgggtctac atctatttgc tttgcataag aatatccaat ccctaaaact
    14101 gcaagtagag tcattacttc aaatgttgct acagttggtt gttgcaattt tacagcccaa
    14161 tctggaccaa aaatttgacc tacaaattca ttccatcctt ttataggaaa atttgctact
    14221 aataaaaata atgaacctat tattaataat ggtgatgata ctaaaaaacc atctctaatt
    14281 gcaattaata atttattttt acctattttt tcagccaatg gcataagtat tctttccaat
    14341 ctattcatct atccaccctc atttcctatt attttttact ataattctac gcttgtttag
    14401 attttattag ctttatagca agttttaata ctttttcccc attcatagtt ccataatcaa
    14461 cttcattgat tatatctata agttttcctt gagcttcaaa ctttggtttt aactccttta
    14521 gcatgtattt aacttgtggt cctaataata tacaatctgg atttttttct tcaactattt
    14581 tgtcaatttc tgctatcgga aatgcctcta cttctattgg taaattgtgt tcatctgcta
    14641 ccttctgcat tttatctgca agtaaacttg ttgacatacc aaagctacaa aataaatata
    14701 cttttctttt cattttttaa atccccctta atttttttat tttaattttt gaatttcttt
    14761 tcttaacata atcatttctt ttataaggtt tctctcactc atacaagtca ttaaatggtc
    14821 ttgagagtga atcaataaaa tagatatatc tgatttatct cccccagctt ccttttgtat
    14881 aagttcagtt tgtactttat gtgcttttaa aatttcttca tttgcatttt caattaattc
    14941 atctgctttt ttaaaatcgt tttcctttgc atagtcaagt gcttcataaa gcatagattt
    15001 tgcatttcca gcatgaatta taatctttaa aactatttct tccatttttt cctcctaagt
    15061 tgcttctcta cttttagatt atttatacct tacaaacata taatatctta taaaaacaaa
    15121 aaaacatgca caactttttc catcacatgg aaaattttgt gcttatccat agttcactat
    15181 gaattttata taagaatatc ttttaccttt tcaatgtcac atttacttag aatatctagc
    15241 atattttcat cctctgttaa attagatatt tctttaaata attctctaaa tacttttcta
    15301 tcatcatctt ttttatatgc taacaaaagt ataatcttta tatcctttcc atttctaatt
    15361 tttattggtg tttttaacct tgcaaaacat aaaacagatt gttttatata tttcatgtct
    15421 ccatgaggga ataatattcc ttttccaaca ttggttggat atgacttttc tcttgttata
    15481 atagattgta aatactcttt ttctgcataa tgtttttcaa ttaataaatt agttaaatat
    15541 gttatagctt cataaatatc atcatgttct aactctctta ttaaataatc agacatcatt
    15601 aatctgttca taagtttata tgaagtgtct ttttctttta ttttgtttgt gaactcttta
    15661 agtttattct catcctcatc agtaaataat ggtgttgtta taaaaattgg tttttggata
    15721 ttttcatact cttgaaatgc aattataaaa tctatagtct tgttaaagtt atctcttttt
    15781 aaatcttcct ctcttaatgt ttcaataatt tttatgttat caaatctctt ttctatcttt
    15841 acttttaata gcatacttac tccaaaacta aatggacata caatacttgc ctttttattt
    15901 gattcccttt tgctgtattt tctttctaga gaggtttgaa agtgaagtgt aagataacca
    15961 atttcatctt ccgttatttg ttttcctttt aattcaattt ctattgaact tgatattact
    16021 gtaaataaaa aacaaaattt agtttttatg ttatctaaaa gtggattttc taagtaaaca
    16081 ttgcttttca tttgatgcat tgtaacattc aaatgacata ttaattgttc ataaagtaat
    16141 atatcattat taaaatttat tcctgattct tctgatacta aatttataat tttttttgta
    16201 taacttgcta attcttcatt tagatataaa gattttttaa tattattact atttatttgt
    16261 ttgttcatac ccaatattaa agattctata aacaattttt cttcataatt taatactatg
    16321 gataaattag atttcaattc atcttctata tccaatataa gcatttttag cttaagctct
    16381 ttcttagtat ttgatacttt taaagaattt cccagcctta ttcgtattat atttataagc
    16441 aaaaatagca tcaattgctt gaatgatata tctgtaagtc ttatatccat atcctcctct
    16501 atagtaagca ttatgttttt tacaattatt atatctacta tatggaagta ctctatttca
    16561 tctatgttta atctgtattt atctatatat ttaaataaga atgatactaa cattattctt
    16621 atgtctttct catccccttc aatatatata ccactgcctt ttttagtcga gatatttaaa
    16681 ttataattat ttaatgctac tgataattta cttatttctt ctctgacaat atttttattt
    16741 atgtacaact tattagataa atcatttaaa gtaacttttt tattattaca aagtagtaaa
    16801 tttaatatct caacttgctt atgaagctca ctatttactc ttttaacttc acctcgcaac
    16861 ttctgattta ccagtatttt gtctgttcca tctccactaa atttaatccc aatattaggt
    16921 tttcttttga tataagcatt acaaaaagta gataaccatt catcaatata tttgcaatca
    16981 tttcttattg tcctttctga acactcaaaa tcattggcaa tcttattaac atttataaac
    17041 tcttctttta atagcaatgt tgttaataaa ttttcttgtc ttttattcaa acatatcacc
    17101 tctttatgac tcaacctaaa tttattatat ataaaaaaga gctatacata aaggtatgct
    17161 agctctcttt taatatgata tttagctgac ctggtttttg accccacact cgcctactgg
    17221 agggttcgct tgggttcgcc gcactactac tctctctcga ttttattatc ggtaggtctt
    17281 acttctaagc cgcaccatga tcattaaccc aatttttagt gttaacttac gtggatccct
    17341 ttgcctgcga ctggcttgga ctttcaacca cagacctaaa tatcacttat tcattttaat
    17401 attttaaact aaaattgtca atatataaga ttattccata atataattat catttgcttt
    17461 tgtaagtaga gtcaatacaa aaaccttatt agcacctgat aactttaaaa gtttagaaat
    17521 ttcgtttgtt gttagaccag tagtaaatat atcatctatt aaaagtatat ttttattatt
    17581 tattaattta acattttctt taacaacaaa tacatttttt aattcttctt ttcgttcttt
    17641 cttattcaat ttatatagca ttcttgtata tttttttcta cttatgcaat ctaataatgg
    17701 aatatttact attttactta aattaaatgc tattttttgt gcctgattaa aacctctttt
    17761 atttagcctt tttttatgaa gtggaacaaa taatatataa tcaaatttta tgttttctaa
    17821 atataatttt tctttcatta tttgagctat atacttagcc ataaaggttt tttggttata
    17881 cttcagccct aacaccatct tttttgtcac atcattgtat tcaatacaag atattgattt
    17941 atcaaaataa aagtctttat taaaacaata actacactct tcaataaatt ctttttcaat
    18001 tgaatgacgt attattggtt ttccacactt catgcaacca tcttgtataa aattcatttc
    18061 tttaaaacag cttttacata ttgaataagt atttgttttc tttatggatt tatcacaaat
    18121 tatgcaactt atattctcag gatatataaa atccaaacat ttattaatca tatttttaaa
    18181 attcttaata aaattatcat tgcatatttt catatttatt ctatcaacaa cccttcttgt
    18241 ttaaatttat ttagcttata tcctaaattt gaataccttt gattaactct attatttttt
    18301 atcatatatt caagatattt cacatctcca actaatacaa ctaatttttt tgctcttgtt
    18361 actgctgtat ataataaatt acggctaagt aacataggag gagcccacgc tattggcaat
    18421 actacaactg gaaattcact tccctggctt ttatgtattg ttgtacaaaa actatggtca
    18481 atttcatcta attcatcata taggtatgat acaattttag tctggtcaaa taatatataa
    18541 atagttttct tatctttatc tatgtgataa acatatccta tatctccatt atagatacct
    18601 tctccacttt ctttttggtc ttctgtttcc catttttttg tataattatt ttttacttgc
    18661 attaccttat cgccaactct aaataatctt ttagaaaaac tttcttcaac tttaaatttt
    18721 tcttttttgt taagatactt ctgtaactct atattgagat tagtaactcc caattcacct
    18781 tttctcattg aagataaaac ttgtatatct tttaatttat caactttata aaatttaggt
    18841 aatctttcat taaccaatcc tattatttca tttaaaattt cttcattagt tgattttctt
    18901 ataaaaaaga aatctttacc ttttgtattt agataaagtg gttctccatt gtttatctta
    18961 tgggcattta caattatcat actttcttgc gcttgtctaa atatttcatt taatttaact
    19021 acatttataa tattagaatt aatcatatcc ttaagtacat ttccagcacc tacagaaggt
    19081 aattggtcac tatctccaac taaaattact cttgttccta actttatagc tctaagtaaa
    19141 ttgtacatta agataatgtc taccattgat acctcatcaa caattattac atctgaattt
    19201 attgggtctt cttcatcctt aaaaaaagtt aaatcatcat ctgttgaaaa tcccatttct
    19261 agcaacctat gtatagtttt tgcttcttta ttagaagttt cactcattct ctttgctgct
    19321 cttcccgttg gagctgccaa agtcacactt ttaccattat tttcaaatat atctatgata
    19381 gcattaatgg tagttgtttt accagttcct ggtccaccag ttataattaa tactccacta
    19441 ttaacagatt ctttgactgc taaaatttgt ttttctgcta acttaatttt cttatcttct
    19501 tctaaaacat taatctcgtt atctatatct atttttaagt cttcaaattc atactgtgat
    19561 aattttacta tttgactaca tactccattt tcagctaaat agtatggcat aaggtatata
    19621 agattttcat tgtttacttt ctctatatgt attttttgat tataaactag catcattaca
    19681 cactctttta ttatttctcc atttagctcc aataattttt ccgaatcttg tattagaaca
    19741 tgctctggta aatatgtatg cccattactt aaagatttat ttaaagtgta taagattccc
    19801 tgacaaattc tatctttaga gtttttatct ataccaattt tacttgctat actatcagct
    19861 actttaaatc caattcctct tatatcttca gcaagttggt atgggttttt atttataact
    19921 tctattgcac tgcttttata ttttttatat atttttaggc agtaatttgg tgttattcca
    19981 aaaggagata gctgtattat tatatttctc aattctctgt cttcttcata actttttact
    20041 atttgtttta cttttttact ccctatacct tctacttctt gtagtttttc tggtgaattt
    20101 tgtatcacct ctaatgtatc tacaccaaac ttatcaatta ttctttttgc cattttttca
    20161 ccaataccat gtatcattcc agaagataag taaacataaa ttccttctaa ggatgatggt
    20221 gttactggca taaaactatt cacttcaaat tgagtcccat aaatcttatg atttacccac
    20281 tttccttcaa cttctatgct ttctccaatt gccaaagtag gcatacatcc tacaaccaca
    20341 atttcatcat tttcatttac caggtgagct attgtataac cattatcttc gtttttaaag
    20401 actatttcac ttatcattcc ttctaatttt tccattttta tctcctaaaa atactaataa
    20461 tcgctccatt taaatgttag tataatttta tatattaaaa ccatgataat cgtagaaaag
    20521 ttttatattt ttatacttaa cgtaatttcc aactattata cattttatca gaaacaaaaa
    20581 aaatctgcat ccataaaata tggtaccatg aaatacaggt accatattta aatagctatt
    20641 ttattttaat tactctatat ttagagtata aattaaattt cataagtata ttacagtact
    20701 aacttatttc agatttattc tccttgtacc cctcagtttt agtaattctt ctataaagca
    20761 tacatagtaa aattaaaatt cccatgtaac ctgtatatgg atatagtgta cctactaact
    20821 tatcaaatgg cagtaaacct cctatacaag ctaatatact taccactatt gttataattt
    20881 taaacttagg gtggtcatct tctacaaatc ggtttgttac tgaccataac aatggtacag
    20941 ctgtagtgta tattcctagt aaaagtacta ctgagaataa aatacctatt actggagata
    21001 tcttatcagc taagtaaagt gctggtatct cttttgtgta tatatttcct atgtctgaaa
    21061 gtaatgccaa attcatcatt atagctgctg ccataagtgc tactccacct actataccac
    21121 cccatacagc atctttctta tttactgcag aagctcccat tcctgtcaag aatgttatta
    21181 ctattattac attgtaacaa gtataaagta ctcccgagaa atatccattt gatgtagcag
    21241 ttttaatatt taaactatca accatatttc cagcatttga taaagcatca atattgctaa
    21301 ataaactaat tgctcctact aaaagtgtaa atattattat tataggtcct atattaccta
    21361 gtattttaga aagccttgtc aaacctaatg aaacagttat tagggaaacg attgccatac
    21421 caatcctacc tacatatgga tttaatccat agtactctga taatgtagct cctgctcctg
    21481 caatcattat tactaatgtt ccaaataaaa acaatggacc aaaccattca aaaaatgttc
    21541 caagatattt tccacaatat acttgataaa tttttattgg ctccttaagt ttcaattctc
    21601 ttcccttatc tataacttct gctccaaacc atgaaaatag taccatagag attattccac
    21661 ctataatacc cgcatatcca taaaacgtaa aaaattgcag tatttcttga ccagttgcaa
    21721 atccagaacc tattacagta gcaacataag ctccagcaaa acttataacc gttttaatat
    21781 ttactttgcc tgacatacac tactcccccc caattaaaat tatttttaaa gtgtaagttt
    21841 attaagatta tattagactc attattgtga tatttcaata tatttttaat tatttaacat
    21901 tagttaacac attcttaacc atcctttaac atatatccca taatatgtat aaatttagta
    21961 gagtattata taaaatgtat tgaccagatt aaatactgtt tattcattat atgatgataa
    22021 attaacattt attaaaaata tttaactcat aataaaaaag ctgttagaaa gtaatcgtaa
    22081 aattacattc taacagcttt ttagttaaaa attattcacc taaagcatct tttcttaact
    22141 gctcaacctt gtcagttctt tcccaaggta aatctatatc agttctacca aagtgaccat
    22201 aagcagcaac ctttttataa agaggctttc ttaaatctaa gtctcttatt atcgctcccg
    22261 gtcttaaatc aaagtgttta tttactaatt caacaagttt ttcttctgaa accttgcctg
    22321 tcccaaaagt atctataaat atagataatg gtctagctat acctatagca tatgcaagtt
    22381 ctatctcaca tttgtctgca agaccagccg caacaatatt ttttgcaaca tatctagctg
    22441 cataagcagc agatctatca acttttgtag ggtcttttcc tgagaaagct cctccaccat
    22501 gtctagaata tccaccataa gtatctatta ttatttttct tcctgtaaga cctgtatctc
    22561 cttgtggtcc tcctataacg aatcttcctg ttggatttat ataaatttta gtctcttcat
    22621 caagtaattc tgctggtata acttccttaa taacatgatt aattaaatct tctcttattt
    22681 tatcatttga tactgtttca caatgttgag ctgatataag aactgtatgt actcttacag
    22741 ctttacttcc ttcatattca acagtaactt gagtttttcc atctggtctt aaataatcaa
    22801 ctaaacctgt ttttctaact tctgtaagtc ttcttgataa tttatgtgcc aaagatatcg
    22861 gaagtggcat taattctgga gtctcattac atgcaaatcc aaacattata ccttggtctc
    22921 cagctccaac tttttctatt tcttcttcta tctcttctcc agtcttactt tctagacctt
    22981 catcaactcc catagctata tctccagatt gttcatcaat agaagttata actgcgcaag
    23041 tatcacaatc aaatccatat tttgctcttg tatatccaat ttctcttact gtttctctta
    23101 ctaatttagg aatgtctaca taagcagatg tacttatttc tcctgctact aaaactaaac
    23161 cagtagtaac tgtagtttca caagctactc tagattgtgg gtctttttct aataatgcat
    23221 ctaatattga atctgatatt tggtcacata tcttatcagg atgtccttct gtaactgatt
    23281 ctgacgtaaa taaatgtctt gccattttta ttccccctta tttattctta atttttatta
    23341 taggttttac cttattttta attttagaaa tttactcaaa ataaaaaaac ctctccttta
    23401 taagaagagg ttaataatcg taattacata ctaattaact gacctcatct ttcaagactt
    23461 ttgtcttgta ggaattagca ccttttccca tttgggtggt tgctgggctt cacagggcct
    23521 atccctcagc ctctcttgat aaggtaatca agatttaagt tttgttttac tttactagct
    23581 tactattttt ttaaaatttt gtcaatattt ttagtcatat tttttctatt cctttttttg
    23641 taagacaaat aaaattttct atctatctga aatttaaagt taggtctttt taatgctttt
    23701 tattcatata tttttttgaa gggggtgttc ctattgtatt tagctaaagt aaactacagc
    23761 gatgatgatg ttatacagat gctaagttca gttctaaaaa ctataattaa tgaaaatacg
    23821 attgttgttt gcataggaac agacagggct ataggtgata ctttaggccc tttagttgga
    23881 acaatactta agaacagtaa ctttaaatac cccgtttacg gtacactaga taaccctatt
    23941 cacgccttaa atatatatga gtctttagat actataaaaa atacacatat acaaggtaac
    24001 ttcttggcta tagatgcttg cttggggtcg cagagtaaca taggtaatat acagattaga
    24061 gaaggaccta tcctgcccgg aaaaggtgtt ggcaaaaaac tacctcaaat aggaaattat
    24121 tctatagtag gaattgtaga taaaattgat gaaaataata aactttcatt taataatata
    24181 cgccttagct ttatattaga tttagctgaa acaatagcct tggctttatt agtatccacc
    24241 taatctaaat ataaactaaa tgataaaatt ctttatataa aagggtagat attttagagt
    24301 atctgattaa atactccaaa ttatctacct ttttactttt atatttcaag tttttatttt
    24361 tctactaaag caaacattaa ttcaccttta cagacaagtt cttcacctac atatgcctta
    24421 gcatctgcct tagctatatt ttttctaaga gttgtcattt caacttccat agttaatgta
    24481 tctcctggtc ttacttctct cttgattcta actttatcta tacctgcaaa tacacctagt
    24541 tttcctttgt tttcctctac agacatcatt gccactccac atacttgtgc tagagcttct
    24601 actattagta ctcctggcat caaaggatat tctggaaagt gaccttggaa aaatggctca
    24661 ttaactgtta cattctttat accaacagct cttttcccaa cttctagctc tgtaatcttg
    24721 tctactaata aaaatggata tctatgtggt attaattttt ttatttcatc tatatttaac
    24781 attctaaact ctcctaatta ttttcttttg aaagacccac cagtttcaag taagtctatt
    24841 gaacctaaag ctattcctgt accttttgca acacaagata atggttcatc tgctattgta
    24901 acagttattc ctgtcctctg ttctattatc ttatctaatc catataataa tgaacctcct
    24961 ccagtcatta ttataccgac atctcctata tctgctgcta actcaggagg cgttttttcc
    25021 aaaacagcat gagtagcaac tactatttgc tcaacacatt ctctaagtgc ttccaacatt
    25081 tctgttgaat ttatagttat agaactagga agtcctgtaa ttaaatttct tcctgttatc
    25141 ttcatatatt tttcttcttc tctcttaaat gcagaaccta tttcaaactt gattttctct
    25201 gctgttcttt ctcctataag aagattgtgt tgttttttca tatatttaac tatagcatca
    25261 tcaaatctat ctccaccaac ctttatagat tcacttacaa cagctcctcc tagagatatt
    25321 acagctatat ctacagtacc tccaccaata tctataatca tattaccatt aggctgtgat
    25381 atgtctacac cagctcctat ggcagctgct attggttctt ctatgatata tacttctcta
    25441 gctccagctt gtctcgtagc atcttctact gctctttttt ccacttctgt gacacctgtt
    25501 ggtacacata ccataattct tggcataaaa aatcttccaa atcccttttt atcaactatc
    25561 ttatctataa aagattttaa catcttctca gttacatcat aatctgaaat tacaccatct
    25621 cttaaaggtc ttatagcgac tatattacct ggtgttctcc ctatcatctt tttagcttct
    25681 tctcccacag ctaaaacaga atctgttctt ttgtctatcg ccacaacaga tggttcttct
    25741 aaaacgattc ccttaccact aacataaaca agcacatttg cagtacctaa atctatgcct
    25801 atatcagctc cagccatttt ttcaactcct ttatttataa tattagatta tattataagc
    25861 ctattttaat actcctataa taaagcatat acaaaaatct cgctttaatc aagagaaaat
    25921 aaatcttttg agagtattat tattttgcta tttataatta cttaatatat ttctttttat
    25981 aatttaattc aataaaaaaa gtaggttttt aacctacatt tttttatgtt ccttctcata
    26041 ttttaatttc gttgctaatc cccctctaat atgtctctct gatttatttt tgtccagcac
    26101 atttttaact tctttggcaa gggatggatt tatttccttc aatctttctg taacatcctt
    26161 atgaattgta cttttactta ctccaaatgt cttggcggtt tgtcttactg tagtgttttt
    26221 ttcaagtata tattttgcta ccactatggc cctttcctct atatgagatc tcaaagatta
    26281 cccccctcac ataatagctt taatgatata tatgtaaaaa caccaaaaaa tataacaagc
    26341 ttaaccaaat atgattaaac ttgttatact attacttaat tagactcatt gggtctatag
    26401 atttttctcc actatacgcc tctaaatgca catgtatacc ttcttcactt tctatttgag
    26461 aagtactacc tacagtacct agagattgtc cttctgttac cttctgttct ttcttaacac
    26521 taacattttt atctagattt gaatatacta ctacaatgtt attgtcactt tttattttga
    26581 ctgattgtcc atattcttca tcatcaaata catctactac tattccgttt aatagagatt
    26641 ttacttcttt gcctttagta caactaatat ctacaccttt atgagtttcc caaacatcaa
    26701 gtgttttaga ataactaggc tctttttctg aatatcctct tattacttta tctccaatgt
    26761 aatttaattt tgttgtactc gatttatttt cctcttttga cttagctttt tctaagtttt
    26821 gttttgaatc cgttgcagta gggatagcat ctttcttgtc tttttcaatt aaatgtattt
    26881 cctcttcact atctttatta gcattttcca taattccttt attagaagcc aatttatcta
    26941 cattattatt tgtaaaccaa actccaccta ctgctaataa acaaacacat acaaacaaag
    27001 ataaataaaa accatctttt tctaacagct ttttcttcat tttgcacctc caaatatatt
    27061 tctacatttg gattattgac gtgtttttgg cattttatac aattattagt atatatcttt
    27121 tatttttgta tctgtgtagt aatgtgataa tatatcataa tatttatagc cctcttctgc
    27181 cattccttct gctccccact ggctcatacc aacaccatga ccatatcctt ttaccacgaa
    27241 atctatgtat ccttctccaa attttatgtc aaagtttgca gagttcaatt taaaaacagt
    27301 tctaatgtct tttccagtca attctttatt tcctaatttt attttttcta cagttccggc
    27361 atcacttctt ttagttattg aaacttgttt acttaaatta ttcacatcta tcacaatagt
    27421 gctatatgcc cttctaagac ttttaacaaa atctgtgtta cttattttaa gtgttgacgc
    27481 aaatttaggg gaatacttat cataaggact ttccacagac tttaaataag gatattttgc
    27541 agaaaatact tcttcactat tttctgtttt tcctgaagat gtagaaaaat aaagaggaag
    27601 tatcgcctta tcattataag ttattatttg acctttagtt cctctaactg cttcttggat
    27661 tttggaatat ttattcttaa tccattcttc accatttaat tttttaagag tatcataact
    27721 cttatattct tgacaatgtt tataatcagt acatactact gcatttttat gtttactaga
    27781 tttaccatgt tcctgtttat atactacata tgttctagct gctacagatt gagcttttaa
    27841 agcttctata tcaaactctg aagacatctc accagctagt actccacata aatagttctc
    27901 catgtccatc ttttcggttt tccctgttat atgattatat acattgataa ttggtgcttt
    27961 tttatttaca gtttcatagt tcactgtatt tttttcttct ttattacctt tgtcttcaat
    28021 atcacttttt ttaatagttt tgtttataga tactgggctc tctggttttt cagttaattc
    28081 tacattttta taagaaacta aagttataag tgatggaact aacacagagc aagtcacaaa
    28141 tcctaacaaa acaaccaatg ggttcttcat atactctcct ccttagcttt taaaaccttg
    28201 tatttaagta tatgaacaac cctattaatt tataactttc ttctataaac caattattaa
    28261 tcttctacta tttcgatttg accacctagt gctgttatct tcttatctat atcaacataa
    28321 cctctttgga tatggtatat ttctcctatt tgagtttctc cttctgctat aagaccgcat
    28381 aaaataaggg ctgcccctgc tcttaaatca gtagcgttta ctgctgcacc gtgtagctca
    28441 tctacaccat taacaaccgc acttcttcca tctattttta tattagcacc cattctatta
    28501 aattcagcaa catgcataaa tctgttttca aaaacagttt ctataactac accagttcca
    28561 ttggctacag taagcatagc cataaattgt gcttgaacat ctgtaggaaa tcctggatgt
    28621 ggtaaagttt ttatatctat tggttttaat acttttggac ctacaactct tacagaattg
    28681 tccatttctg ttatttcaca accagcttct cttaatttag ctacaactgg ttttaaatgc
    28741 tccattaaaa cattttcaac agttatatca ccttttgtca tagctgctgc aaccatataa
    28801 gttgctgctt ctattctgtc tggaattaca ttatgttctg ctccttttaa ctctttaaca
    28861 ccttttattt ttatagtatt agtacctgca cctttaacat ctgctcccat ttcgttcaag
    28921 aagtttgcta aatctactat ttctggctct tctgctgcat tctctattat tgtagtccct
    28981 tctgctaaag aagctgccat cattatgttt tcagttgctc caacagatgg gaaatctaaa
    29041 tataatttat ttcctactaa cttttctgtt gctgcttcaa caaaaccata gtccatttct
    29101 atctttgcac ctaaagcttt aaatcctttt aaatgtaaat ctataggtct tgttcctata
    29161 gcacatcctc ctggcataga aattttcgta ctattaaatc tagcaagcaa tggtcccata
    29221 accaaaaaag aagctctcat ctttcttaca agctcatatg gagcttcaca agtttttata
    29281 ttgcttgcat caacagtaag tgtattttct ttatattcaa cttctgctcc tacatgtctt
    29341 aataaatctg atataacatg aacatctctt aaatttggta ctccatttaa tgttgatttt
    29401 ccgtttgcta ataaagtcgc tgctattatt ggtaaaactg cattttttgc accatctatt
    29461 ttaacactac ctttaagtgg attactcttt tttactatta tcttagccat attttttgat
    29521 ctcctcataa ttaataatct aattttataa ttggtgtggc aatatatatg agtgtttttc
    29581 cgtcatcctc attatattta attcctatat ttaaattaat tttttcccct aaatattcta
    29641 attcataatc atttaatact ttactatatg ctgttataga taaaaagttt acgtctttaa
    29701 cttcatctat ttgcttagca ctcatattat ataatatttt ttgtaaaata tcatcatatt
    29761 tgttgttttg taaccttttt gtgtattctc caacaataca agcagacaca tctacttgag
    29821 atgaatattt atttaacaca ttttctacgt tactataaat aacctcttta tgtttatata
    29881 ctttgttatc caatatgtca actactatgt aactttcttt gttattttta tttattcctt
    29941 ttatagaaat ttctttatcg ctacctttta tagtagcaca taattgtatt ttatcgccag
    30001 accctttttg attccaattt attccttttt tatccaactt tagattgtta attatttcta
    30061 taaaaatatt atgaaattct ttatttgata aattatgatt tataacagaa ttaattttaa
    30121 cattataaaa tttaaaattt gcttctgata tttcaagagc tttacttaat ctattgtatc
    30181 tagtatttat acttgtatct gcataagata ttgtcattcc tataatcatt aatataaaaa
    30241 agcttatagc taattttact tttttcatag taaaaaatcc cccttaaata tgtttttctg
    30301 tataatagaa ttattaacaa atttaagaga gatatacttt ctcaatatgt atttttatac
    30361 atttttgtat ttttcatcta ataatctctt tcaaactctt caatctcaat tttctctatc
    30421 cttctcctgg acttgccaat ttcttatatg tatcaaatac atcttgcatt aattcttata
    30481 tattctcttc tgtaatataa attacttagt ctatacttaa atctataatc tattcatatt
    30541 ttaccaacaa aataaacaga taatactttt ttatcaattt ccatagctcc aaatgaacat
    30601 acctaagtac acttcccaat acacaattta cacactcttc ctaataaatc aaaaacacct
    30661 ttgttttaaa caactctatt gcactttcaa aaactttatt tgtgttctcc cacttcaatt
    30721 aaatttgaat tatcaagact ccagcccatc catccatcac tataaaggga ggtattttcc
    30781 actcctatga catttgcata tgttaatact tcagcagcac gccatccact tccacacata
    30841 aatataagct gtttattcac atctatattt tccttgtccc acatttctaa aatctcatat
    30901 ttatttctca ttgtattatc aatattacga tattcttcta gtgaaacact atcactacct
    30961 gaatgtccat ataaagctcc tggtatacgc ccttttttat cataatatgt atatccagat
    31021 acttttccta tatgttcatc ccaagtacgg ttatctacta gtataaactt atttttttct
    31081 tttagttttt gtcttaattc agatgtagta acaattaatt gtgagttaac tggtatatct
    31141 gctccaaaat tagtacatga atgttttgga ttactaataa attctaattc atatcctgct
    31201 gataaccatg agtttgttcc accattcaaa actcttacat cttttactcc tatatacctc
    31261 aatataacag ctagtctata agatgccata ggtgtactac ttgaaactat tacagtatca
    31321 tcctttgtta atccatagtc taaagcaaac ttagttaagt tatcatcatt atcaagcatc
    31381 catgttggag gaggttctat aatatcagta tttacatgaa cacttgtagg tatatgccct
    31441 tttgtatacg attcttctcc ccaactcgct tcaatcattt ttatattctt tgaatcttca
    31501 aatgtctcag gaatttttcc atctaatata tcttttatta taaaagcagg aactatcatt
    31561 tgataatttt tatatctttc cattggaaga ttttcgtcgt ctgcccattg tttaatatca
    31621 tatttatata gatatttata tcctttttta gataaataat ctgctacaac caaagcatct
    31681 tttccatttg catcatacaa tactatattt ttatctaaat caattctttt agttttcaat
    31741 gcttgttcta acacttcatc ttttctatct gagtatacac ttaaccaatt agcagaaaaa
    31801 tctactgctc cttttatatg tccacctctt tttactccat ctaacttcca gccattataa
    31861 gcatcattta agcgagtatc aactataacc caagcgttat cttgcacttt ttcttgtaaa
    31921 acttcagtag atattgcctt aagattaaca aaataatctt ctttcttttc ttttttaaaa
    31981 tcatttgtca taatttatac tcccaattta aaaaaatttt tatttgaaat tattttacta
    32041 aaataaagta attttcttat actctattag cttcattata ttcttttata aatagttcct
    32101 tatattctta ttttaaacta ctcactttat cttatcacaa ggttactaat accttgtatt
    32161 ttaatttttc tcataagcta ttatttttta tgtctgatat tgactatatg tttattaaat
    32221 actcactata ttttatatga actatattta tcgttcccta tacctggtat ggtctatata
    32281 tttatcattt tacagatgat ttaatatcaa tctttttata aaatttatta tagcaaaaac
    32341 aaaacaaatt taatatttat ctaaaaaact catcctataa aatacaaata acaggatgag
    32401 ttcttttcaa taaatattat ctatttaatt tttaatctgt ttttattcaa ctgttactga
    32461 tttagctaga tttcttggct tatctacatc gcatcctctt agtacagaga catgataagc
    32521 aagtaattgc attggtacaa ctgctaatat tggtgataaa atatcatctg aatttggtat
    32581 atagattatt ttatcagcag ctttctcaac atccttatta tggctttgag ctattgctac
    32641 tacataagct cctcttgctc ttacttcttc catattagaa accatttttt caaataactt
    32701 ttcttgagta gctatggcaa ttacaggagt tcctttttct attaaagcta ttgttccatg
    32761 ctttagctct cctgctgcaa aagcttctgc atgtatatat gaaatctctt ttaatttcaa
    32821 tgaaccttcc attgctaagc tatagtctat acctcttcct aaatagaatg catgttcaga
    32881 actaacaact gttttagcaa cttcttttat atattcttca ttgtctagta tttcttgtat
    32941 ttttgatggt atttctttca tcttagaaat catactatca taaaattctc tagttattgt
    33001 tccttttttg atagcaaagt ctaaagctat catgtataaa gaagttattt gagtagtata
    33061 tgccttagtt gatgcaactg ctacttcagg tcctgcccaa gtatagaata catcatctga
    33121 ttctcttgct atagatgaac ctacaacgtt agttattgaa agtattcttg ctcctttagc
    33181 ctttgaatct cttaatactg ctagagtatc tgctgtctca ccagattgac taactaatat
    33241 tactaatgaa ttttcatcta caaatgggtc gctatatcta aattctgatg ctatatcagt
    33301 tattactggt atatttacaa atttttctat ggcatactta ccaagtaatc cagcattata
    33361 tgctgttcca caagccacta tataaacttt atttattttt tctaagtctt ctttagaaat
    33421 atttatactg tctagaatta tatttccatt atcatcaagt cttctttcta aagtttctct
    33481 tactcctgtt ggttgctcat gaatttcttt tgacataaag tagtcatatc cacctttaga
    33541 cgcagcttct acatcccaag ttacatgaaa tacttctttt tcaacaagat ttctattaga
    33601 gtcataaact gttacatttt cgtcttttag atgaactact tctccatttt ctaagaaata
    33661 aacatctcta gtatatttta ataatgcagg tatatctgat gctataaagt tttcaccttc
    33721 accaacacca actacaagag gactatcttt tcttactgca actaattcat ttccatgttc
    33781 tttacatata acacctaatg cataagctcc tcttaatttt gatatagttt tgtatacagc
    33841 atctaataaa ttaccctcat agtatttatc tactaaatgt gctattacct ctgtatctgt
    33901 ttgagattca aacttaacac cttcagaaat taattcttct tttatttcca tatagttttc
    33961 tattatccca ttatgaacaa ctgctattgt tttggcttga ttaaaatgag gatgtgagtt
    34021 tacatcagat ggttctccat gagttgccca tctcgtatgc cctataccta aatttccatc
    34081 aataggattc ttttctaagt cttctgcaag tacagaaagt ctacctttaa actttctaat
    34141 attaagttct ttttcattag aactattaac tgctactcct gctgagtcat aacctctgta
    34201 ctctagctta gaaagccctt ctactataac ttctgctgcc tttctacttc ctaaatatcc
    34261 aactattcca cacattattt gtgtcctcct aaaaatatta tttatatatt ttaagagtta
    34321 ggtcatataa gtttactttg gcttcccttt gtccatagga ttgctcctat tatttaaaga
    34381 agtctttcga tggtaaacac accgcagagc atccgccgac aattcgatta actctgacct
    34441 cgtcaactta gttcctatat tactcaataa ttatgtctat aactaagttc tggcgcttag
    34501 aaaggtgtcc tcagtattta tatctgagaa taccctttaa attacgataa tttttcttgt
    34561 ataagattag ctaagtttgt tgctaattct tttatttgac cttcctcttt accctctaac
    34621 ataactctaa ctaatggctc agttcctgat ggtcttatca aaactctacc atttccgtct
    34681 aaaatagatt ctattctttc tatctcagtt tttatttctg gatattccat atatttgttc
    34741 ttattttcat tttttattgt tgcatttact aatacttgtg ggtattggct cattattgat
    34801 gcaatttctg ataaaggttt cttttcttgc aagattatat ttgcaagtat tagagaacta
    34861 agcactccat ctccagtagt gttgtaatct aaaaatatca tatgtccaga ttgctctcca
    34921 ccaagattat atccattttt aaccatatct tctaaaacat atctgtctcc aactgctgta
    34981 gtagataaat ttattccatt ttccttagca gctattgtca accctatatt actcataact
    35041 gtaactacca aagtatcttg tgccaattta ttatttttct ttaaatttaa ggcactcaat
    35101 atcattatat ggtctccatc aacaatttga cccttttcat ttactgcaat taatctatca
    35161 gcatctccat catatgcaag acctaagtca gcattatgtt ccaaaatagc cctttgaagt
    35221 tgttctggat gtgtagagcc acatttatag tttatattat ttccatctgg tgaactattt
    35281 attgaaatta cacttgctcc tagttcatca aacactattg gtgccacttt atatgacgca
    35341 ccattagcac agtccaaaac tacttttaat cccttaaagt cagtgcttat tattgatttt
    35401 aggtagtcta catagtctct ttgtgcacaa tgttcatgta attttcttcc tacattttca
    35461 ccaattggaa gacaatcaat tttgtctata tcgtctatat attcttctat tttcaattca
    35521 atttcatcat ctaatttata tccattctta ttaaagaatt tgattccatt atattcaaca
    35581 ggattgtgag atgcagatat aaccacacca caatcagcac catattttct agttaaatat
    35641 gccactgcag gtgttggtat tattccaaca gtaataacat cacatccaac agacataagt
    35701 cctgctatta aagatgcttc caacatatca cctgatattc tagtatcctt acctactact
    35761 acttttactc tgtggtctcc ttgagctaag acaaatccac cagctctacc aagtttatat
    35821 gctaaatcac atgttagttc tgtatttgct actcctctaa ctccatctgt tccaaaatac
    35881 tttctcacta aagattctcc tttcataatt gaaaagcttg taatttgtat tatattttta
    35941 tagttcataa aatatcatta taggcttaat ttcagtcata aaaatatttt aatacttatc
    36001 agcaaaaaag acaatagaaa taattctatt gtcttttttt tgacatcttt ttctttatta
    36061 gttagttgct ttagaacatg tagcatccat acctttttgt aaagcttctc tgtttaatgg
    36121 aacgaatttt tcttttctag gtccgaaaac ttttttgaat gcttctagta cagattctat
    36181 atctacagta tcagatactt ttaagtaagc gcctaacatt atcatgttag caactttatc
    36241 atttcctaat tcagctgcta attcatttgc tggtatataa taaacatcta catctgttct
    36301 atcaactttt tcttttatta aagaactatt tactattatt tttccaccag gaataacatc
    36361 atctttgaat ttttcaaatg ctggtatatt taataatata gctgcagttg catcatctgt
    36421 tataagtgga gaaccaactg gctcattaga tacagttact gcacagtttg ctgtacctcc
    36481 acgcatttct ggtccataag atggtaacca agaaacttct tttccttcta gcattcctgc
    36541 ataagtaagt aattgtccca tagacataac accttggcct ccaaagcctg cacatattac
    36601 tctagctgta gacatttact tcacctcctc aacttcaaca tttttgaaat ttcctaatgg
    36661 atagtatggt atcatgttgt ctcttaacca ttgtaaagca tcgtttggag ctagtcccca
    36721 gtttgtagga caagtagata atacttctac tattccaaat cctaatcctg cttgttgaac
    36781 ttggaatgct ttctttatag cttttttagc ttgtctaaca tgagctggag tatcaactgc
    36841 tactctttct acaaatacag ctcctgttaa tgtagctaac atttcactta ctcttattgg
    36901 ataaccttga gtttcagcat ttcttcctga ttgagcagta gtagctcttt gtccaactaa
    36961 tgtagttgga gccatttgtc caccagtcat accataagtt gtattgttta caaatatagt
    37021 agttattttt tctcctctag cagctgcatg aactatttca gcagtaccaa ttgaagctaa
    37081 gtctccatca ccttgataag tgaatactgt attttcagga tgaactcttt ttattcctgt
    37141 agcagcagct ggcgctctac catgagcagc ttcttgagtg tcacagttaa aatatttata
    37201 tcctaaaact gaacatccaa ctggaacaac acctacagca tcacctaata ctcctagttc
    37261 ttctaatact tcaccaacta atctatggat tataccatgt gtacatcctg gacagtagtg
    37321 agtttgagta tcttgtaatc cttcagtttt cttaaataca acagccatta tctagcacct
    37381 cctgcaacca attctccggc aataattttt ttagcttttt caactatttc tgctggtgtt
    37441 ggagtcattc ctcctggtct tccatggaag taaactggta atttaccttc tactgccatt
    37501 ttaacatctt ccaccatttg tcccatactc atttcaacag tcaatatgtt tctagcatta
    37561 ggaatttggt tgaatgcttc gaatgggaat ggccataata ctttaggtct tataagacct
    37621 gcttttattc cttcttctct taatatatca attgcgcttt ttactactct tgaagtagtt
    37681 ccataagctg caaatacaaa ttcagcatct tctgttttgt acatttcgta ttgtacttca
    37741 ttcttttcca ttgcatcaaa tttttcttgt aaatgccaac aatggtcttc tagtacttct
    37801 ggctctaagt atagagagtt tattacatta ggttttcttt ttcctttagt tccaacagtt
    37861 gcccaatctt taggaggcaa ttctcttttc ttctcaggag ctctaaattc aactggctcc
    37921 atcatttgac ctatcatacc atctgcaact accataactg gtgatctgta ataatctgca
    37981 acgtcaaaag cttccattat catatcaaca gcttcttgaa ctgtagctgg tgcgaatact
    38041 ggagttctgt agtctccatt tcctccacct cttgtagaca taaagtagtc agcttgtgaa
    38101 ggttgtatac ttccaagtcc tggaccacct ctcattacat taagtacaac acaaggaact
    38161 tctgctccta ctgcatatgt tataccttct tgctttaaag caactcctgg tgaagatgaa
    38221 gaagtcataa ctcttgctcc tgcacctgct ccaccataaa ccatgtttat cgcagaaact
    38281 tctgattcag cttgaacgaa cgctccacct attttaggta actctcttga taaatattct
    38341 ggtaattcac tttgtggagt tattgggtaa ccgaagaaat atttgcaacc agcttctata
    38401 gctgcttttc caaatgcttc gttacctttc ataagtatct tagccattaa aattccccct
    38461 taaatattaa ttaaattaat ctctttctac tgttataact acatctggac acatagttgc
    38521 acaacttgca catcctatgc atttttccat ttcaaaaaca gttgctgggt tatacccttt
    38581 tttgtttatt acatttgagt ctaattgtat gatttttact ggacatgctt caacacacaa
    38641 tccacaacct ttacaacgtt cttgattaaa agatactttt cctttagcca tttacctaag
    38701 cctccttaaa ctttctatat atttgagctc aaactacatc cagtcttctc tcatatataa
    38761 ttttatagga aaaatttctc cttctaaatc ttttggtaac tgttcaacaa gattttctaa
    38821 acatgcaaca tatcttatcg gtatattaca tttttcagaa acttcttttg caactttctg
    38881 acctcttaaa acatcatcaa tagtagtaaa cctaataaga tgagtattat taattagacc
    38941 tgttaccttt aatttagaag atttttctat ttcttttata tattggataa cttcttcaga
    39001 agtttgagtt ttctccctat ttgcatttat tacaaaaagc atatcataat cgccttcttt
    39061 taacaaatga ctaaatcttc ctataaccct agcacctaca ttatctccac ctaaatctat
    39121 aacagaattg tatgaatggt ctaccatagg agacattacc tctgctgata ctgctggtaa
    39181 atctaatgtt ggagcattaa ttgagctatc tattggttgt atacctaagg atttcattaa
    39241 ctctttcttt tctctagttc taaagtatac atttaccaca tctaaatcgg ctatagcaac
    39301 tttgccagaa accatatctc ttaacttaac aacatagttc atcgcaaatt cacttttacc
    39361 acttccatag tgaccagtta tgattcttat tcttttatca ttagttatca taaacttgag
    39421 cctcttcttc accagttaaa actctaagtc caccttgagc taatgcaatc atttcatctt
    39481 cacctggata aacttttaca tctgctataa atttaactct atctgcaatc atttctgtaa
    39541 acatttttga atatgcgatt ccaccagtta ataatattgc ttttacatct cccttaagaa
    39601 ctgcagcact agctcctatt tctttagaga tttgatatgc catagcttca tatactaatt
    39661 tagctttttc atcaccagct tcaattcttt cttcaacttc tctagcatca ttagtgttta
    39721 agtatgcaac taatccgcca ttacctttta ttttcttttt aatttcatct tgagtatatt
    39781 ttccactaaa gcacattttt actaatgcac ctactggtag tccaccactt ctttctggag
    39841 agaaaggtcc ttctccatct aatgcgtttg caacatctac tattttacca tttttatgag
    39901 ctccaacaga aactcctcca cccatgtgtg caactataag atttatatct tcatatttct
    39961 tgtttatttc tctagcatat cttcttgcta ttgccttttg atttaaagca tgtactacac
    40021 ttgctctact tatttcaggc ataccagaaa ttctagcaac atcttctaat tcatctacaa
    40081 caacagggtc tactatgtat gaaggaacat ttacttcttc acctatttgt tttgctatta
    40141 ttccacctag gtttgaagcg tgttctccta aaactcccac ttttaaatct tcaatcatag
    40201 cagcacttac tgaataagta ccacctttta taggtttaag aagtcctcct ctacctacta
    40261 cagcatctaa ttcagatgtt tttactccac cttcttttag agcttcttct attacttgtt
    40321 tacgaaattc aaattggtca gacaccttct catattttcc tatttcttct gaagaatgtc
    40381 ttaaagtttt ttcaaatact aaatcctcat tatcaaatac agctatttta gttgatgtcg
    40441 aaccaggatt tattgttaag attttaaata ttttgctcat ttgcctattt cccccttaaa
    40501 attatgcctt tgctgccatt aaaacaccta aagctattga gtttagttta gtttcttcac
    40561 tgtctgctct agaagttaat attattggtg ctttagcccc aactataact cctgcatttt
    40621 ttgattttga gaagaatacc aaagctttat ataatatgtt accaccttca atatctgggg
    40681 ctaataatat atcagctctt cctgctacag gatgatttat acctttatgt ttagctgctt
    40741 ctaaagatac tgcattatca attgcaaaag gtccaccaac catacaacct ttgatttctc
    40801 ctctttcata catttcttct agttctttag cttcaactgt atctttcatt tttggattta
    40861 ctttttcttt tgcgcatatt gcagcaactt ttggttcact tatatctaat gaatgtgcta
    40921 ctgtgcaagc attttctatg atttgctttt tagtatttgt atcaggagct aagttcatag
    40981 ctgcgtcagt tacgaaaaat aatctatcat atccctctac atcaaatact gctacgtgac
    41041 ttaatacatt tccagttcta agacctactt ctttatttaa aactgctttt agtattattg
    41101 atgtgtctac taagcctttc attaccatgt cggcttttcc ttgtgaaact aattcaacag
    41161 attttagaga tgcttctgct aaatctttta tatctatcag ttcataattt tcgatatcca
    41221 tgtctatgct ttttgcaatt tctttagtct tttctatatc tcctactaaa atggcatttg
    41281 ctattttttc ttttctagcc atttcaactg ccattaaaac ttctttatct tggcaacatg
    41341 ctactgatat agttttaggt cctctttctt ttgcaaactt aattacttct tcaaaacttc
    41401 tcatgcattt ccacctcatc ttgaattttt tgaaatagaa ctttttatat ctcaattaaa
    41461 ttataactcg ttcagcaacg ttttcctaga gataattgaa atatttaatt caaaatattt
    41521 atttaaatct atttatcagt cttttttcat atatctagcg ctttttagat ttaacaaata
    41581 tctcattgtc cttattttat aatataacta aaataaacaa gatgcaacaa aaacataaaa
    41641 ttaacaaact taattttata ggtttttttg tcgcatcttg taacaactaa atattcgaaa
    41701 tatttgttca ttccacttca acaatattag gttctatatt tattttttta aattgatatg
    41761 ttgtttcata ttttatttta tatttactat cttcctcagg accttgtgat aaatcaatat
    41821 ataatgaaat atctttttta tttaacttac tcaaatcctc cgttgactct acaacaactt
    41881 ttatatctac atcagaaatt acatttggat tatccatatt tgttgggata tttcttaatt
    41941 caatatctcc cgcattatat aaaaattctg cactcaattg ttttgtatca tttaatttaa
    42001 ttgttataga gctatcctca agagttatac cctctggaat ttctaagcta acttcttttt
    42061 cttcaccatt agccaaatca gctaagtcaa taggttttgt cttaatataa cttattttgt
    42121 ctatatcttc tttctttcct tttattgtaa cttgttcctt acttaattta tagtttttca
    42181 gtacatcatt atctttatta tcatttagac ttactcttat tgggacaatt ttttccttca
    42241 atagttctat ctttgcagtc acaaaggatg aacttaatgt tactccttca accttagttc
    42301 catcactatt taatggtatt aattgcaatt ttttagaaaa atcatctgtc tcattaccaa
    42361 catctagatt agctcttaca cttttgactt ctttaactag agttcttgga cctgatacat
    42421 cgacaaattc tttactaagt tcaattttat ttatatttct ttttgaattt ccacttactt
    42481 ctacctttat atctcttttt tcttttatgt ttttttcaag atttactact aaaacatcat
    42541 gcttaaattc tagagtaaca ccttgaggaa tatttgcttt taaatatact tccttctgtc
    42601 cttgtattgg gtcttcaatg gttccatata catgtatatc ttctttttta acactttgta
    42661 cctttgataa actacctttt atatatacat ctgctgttaa tttttcgtct ggatatataa
    42721 caagattttt atcctttaaa tcactcatat tacttataga tacaggtata tcttcaaacc
    42781 ctttagtttc ttcagggtca actacagcca taacatacat ccataatgca attgcactta
    42841 aaaatgatat tatttttatt ttggtattat tttttagtct atttatcatt taaatatacc
    42901 acctttgaaa aaacttcttg tttctgtatt tgtttttaaa ttactacgta atatattcat
    42961 cattctttct ctagaaatat ctctatataa cttacctgct ttagcaattg atacgccacc
    43021 tgtttcctct gatacaatca aagttataca gtcagacacc tcacttaccc ctattcctgc
    43081 tctatgtcta gtgcccaaat ctttacttaa atccttactt tctgtcaatg gcaaaaaaca
    43141 tgcagctgct tttaccttag aatctctaat tacaacagct ccatcgtgta atggtgtatt
    43201 tggtataaat atatttatta atagttgcct agaaatttca gcatcaataa tagttcctgt
    43261 atttattata tcacttattc tagtttctct ctccattatg ataagagctc ctattttttg
    43321 tctagaaagt gaatataaag cttctacaat ttcttctata tttctattga gagtttcttc
    43381 agaagtattt acattctttc caaataaatt aaactttgct cttcctatat gttcaagtcc
    43441 tgctctaaat tctggttgaa aaattataag agcagctata actccataat ctaaagcttt
    43501 aagagatatc caataaacgg tatgcaattt aaaggtgtta ctaagttgag ttgctaataa
    43561 aagaaatatt atacctttaa acacttgttc tgctcttgta tcttttataa acataaatat
    43621 tttgtaaaaa atatacgcta ctatagatat gtcaattaaa tcatatatac tcattcttaa
    43681 tattatatta aaaaatccat ttaaaaaaga atttatatct aacacagttt tatcctcctg
    43741 tttgataact tcttaattat ttacactaag tattatacta tataaatcta actctataca
    43801 aactatttaa cttttatgta aataattcta gttttatatt tggagattgt taaataatct
    43861 ctaaacacat taaaaatatc aacttaataa atacatattt cttattaaag ttagttttta
    43921 ctcaaataac tcaaaccatc aaggcgcgaa tacaaaatag tactcgcgcc ttaattcagt
    43981 tttatgttct aaaatatctt aatatatctt tgtttaatat atattaatta ttataaattc
    44041 tataatccac ttattaacta taaacaaata caaaatctaa atattactta tcatcaatat
    44101 tattccatta gattcatttt tatattaaag taataactta tttcatatct agattagata
    44161 aatttatttt aatatcttac tataaactat cattaaaact tattatgtat aactagatat
    44221 aatctcctac agtgaaaata atacacaata cct
//
LOCUS       DACXMZ010000007        42169 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.007, whole genome shotgun sequence.
ACCESSION   DACXMZ010000007 DACXMZ010000000
VERSION     DACXMZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42169
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.007"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(89..1690)
                     /locus_tag="JD016_01505"
     CDS             complement(89..1690)
                     /locus_tag="JD016_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAU4998304.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKFWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(1801..2478)
                     /locus_tag="JD016_01510"
     CDS             complement(1801..2478)
                     /locus_tag="JD016_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998305.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(2491..3186)
                     /locus_tag="JD016_01515"
     CDS             complement(2491..3186)
                     /locus_tag="JD016_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HAU4998306.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(3238..4437)
                     /locus_tag="JD016_01520"
     CDS             complement(3238..4437)
                     /locus_tag="JD016_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAU4998307.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKYGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(4698..6743)
                     /locus_tag="JD016_01525"
     CDS             complement(4698..6743)
                     /locus_tag="JD016_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAU4998308.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(7154..7963)
                     /locus_tag="JD016_01530"
     CDS             complement(7154..7963)
                     /locus_tag="JD016_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAU4998309.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(8047..8580)
                     /locus_tag="JD016_01535"
     CDS             complement(8047..8580)
                     /locus_tag="JD016_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAU4998310.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(8730..10079)
                     /locus_tag="JD016_01540"
     CDS             complement(8730..10079)
                     /locus_tag="JD016_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HAU4998311.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTHFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEVIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(10091..11473)
                     /locus_tag="JD016_01545"
     CDS             complement(10091..11473)
                     /locus_tag="JD016_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HAU4998312.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            11717..12931
                     /locus_tag="JD016_01550"
     CDS             11717..12931
                     /locus_tag="JD016_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAU4998313.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            13129..15021
                     /locus_tag="JD016_01555"
     CDS             13129..15021
                     /locus_tag="JD016_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HAU4998314.1"
                     /translation="MKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLKEGIPLAE
                     KSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEPFGIIGFN
                     NVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAHIVKRLNC
                     KGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVDKNFKITL
                     FNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTKIITNRVP
                     IIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHTSEKTKDCIEL
                     AKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSLIESEFFG
                     YEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQVMRIGGNK
                     VIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDIIKLSEFFI
                     HKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSILLEKVSNE
                     YTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRSTLWRKLK
                     EIEDDV"
     gene            15292..16266
                     /locus_tag="JD016_01560"
     CDS             15292..16266
                     /locus_tag="JD016_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HAU4998315.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIIALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            16287..16562
                     /locus_tag="JD016_01565"
     CDS             16287..16562
                     /locus_tag="JD016_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HAU4998316.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            16575..17738
                     /locus_tag="JD016_01570"
     CDS             16575..17738
                     /locus_tag="JD016_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HAU4998317.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            18075..20354
                     /locus_tag="JD016_01575"
     CDS             18075..20354
                     /locus_tag="JD016_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAU4998318.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQGISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(20449..21789)
                     /locus_tag="JD016_01580"
     CDS             complement(20449..21789)
                     /locus_tag="JD016_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAU4998319.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(21801..22583)
                     /locus_tag="JD016_01585"
     CDS             complement(21801..22583)
                     /locus_tag="JD016_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4998320.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(22598..23620)
                     /locus_tag="JD016_01590"
     CDS             complement(22598..23620)
                     /locus_tag="JD016_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAU4998321.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(23626..24639)
                     /locus_tag="JD016_01595"
     CDS             complement(23626..24639)
                     /locus_tag="JD016_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAU4998322.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(24767..25705)
                     /locus_tag="JD016_01600"
     CDS             complement(24767..25705)
                     /locus_tag="JD016_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAU4998323.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDATPFFSQSYNPIGKELLLESVKNELTK"
     gene            25975..26439
                     /locus_tag="JD016_01605"
     CDS             25975..26439
                     /locus_tag="JD016_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998324.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQSKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(26551..26868)
                     /locus_tag="JD016_01610"
     CDS             complement(26551..26868)
                     /locus_tag="JD016_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAU4998325.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(26881..27612)
                     /gene="chbG"
                     /locus_tag="JD016_01615"
     CDS             complement(26881..27612)
                     /gene="chbG"
                     /locus_tag="JD016_01615"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAU4998326.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKSVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(27638..28948)
                     /locus_tag="JD016_01620"
     CDS             complement(27638..28948)
                     /locus_tag="JD016_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAU4998327.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(29045..30358)
                     /locus_tag="JD016_01625"
     CDS             complement(29045..30358)
                     /locus_tag="JD016_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAU4998328.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(30380..30691)
                     /locus_tag="JD016_01630"
     CDS             complement(30380..30691)
                     /locus_tag="JD016_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU4998329.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(30891..31595)
                     /locus_tag="JD016_01635"
     CDS             complement(30891..31595)
                     /locus_tag="JD016_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU4998330.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(31843..33501)
                     /gene="treC"
                     /locus_tag="JD016_01640"
     CDS             complement(31843..33501)
                     /gene="treC"
                     /locus_tag="JD016_01640"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAU4998331.1"
                     /translation="MLDFKKSVVYQIYLKSFNDTTNNGLGDLNGITEKLDYLEFLGVD
                     YVWITPFFVSPQNDNGYDVADYYNIDPIYGTMDDLENLIKEAKKRNMGIMLDIVFNHT
                     STEHEWFKKALKGDKYYKDFYIFKNPKEGKSAPTNWESKFGGNAWEFVEKFNQYYLHL
                     FDKTQADLNWENPNVRKELKNILKFWIDKGIKGFRFDVVNLISKPEKYEDDLQGDGRH
                     FYTDGRKVHQYLKEMCEESGVLENDMLTVGEMSSTTIENCIKYSNPNEKELSMCFNFH
                     HLKVDYKNQNKWMLQDCDFIKLKKLLNEWQIGMEKGNGWNALFWCNHDQPRIISRFGD
                     DKKYYKESAKMLATMIYLLRGTPYVYQGEEIGMTNAYFTNIKQYVDVESLNYFDILKS
                     QGMKDGEIYNILQERSRDNSRTPMQWNNSKNAGFSESKPWIEVINNYRNINVENSLKD
                     KDSIFYYYKKLIELRKKYDVVAYGDFKPLLEENDSIFAYKRCYEKESLVVLNNFYDKE
                     IDVILDNEELEKYKCIISNYGDRKVTSTITLRPYECMALFKNEK"
     gene            complement(33564..34997)
                     /gene="treB"
                     /locus_tag="JD016_01645"
     CDS             complement(33564..34997)
                     /gene="treB"
                     /locus_tag="JD016_01645"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HAU4998332.1"
                     /translation="MGKNKILNSKESISQIIEYVGGKENIQSATHCVTRLRLILKDTN
                     KANINKLEGIDIVKGCFNAGGQFQIVIGVGVDKVYKDLIEQTGAEEVSKEEIKQQVNE
                     KSNKLQKFIRVLADIFIPILPAIVASGLLMGVNNMIANPGIFYEQSVIEVNTGIAGIS
                     GIINLIANTSFTFLPALIGWSAAKKFGGNPLLGVVLGLVLVHPALMSAYDFAANPSAA
                     PTWDIFGMKVAQVGYQGQVIPVLIATYILTKLELFLKKTVPDAVQLVLVSPIALLITG
                     MLTFVVIGPITMTGANMITNGVVHLFEVAPVFAGALYSFISPPLVITGMHHLFLGVNL
                     QMAGTLGYVTLWPISETVTLAQGAATLAMFFILKNNKNLRGVSITSTISAWLGITEPA
                     IYGINLRFRYPFIAVMVASAIGGAFISYHNVKATAVGVGGVFSFLSVMPQYWGVYFTG
                     MAITFVITIGLTYLFYKSGKFKTNDNI"
     gene            35245..36984
                     /locus_tag="JD016_01650"
     CDS             35245..36984
                     /locus_tag="JD016_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998333.1"
                     /translation="MNIKEIPFSYFGSYMSISYSDELGFTLKSLRGKSKQNMDVLRII
                     PTVNNIPVDYEFEADYFSILIKFNNGSISICFDGDSKILFYGKGKNVGLKLDTLPVYN
                     FEYNYLMGKKDSEYCVINSYKNLTKFLVFSVDGKVNLSQNIFIDDCASTNKANNTSII
                     DVNNNTDNEFTCIIEDIPTHMKVPDYKEYNFETSLNNTKKLFNEYYLAQPKIDEKYKN
                     SLLRASYINWSSFVKPQGYLKRYTILASNSTFLGAWSWDHCFNALALAGVDDKLAFDQ
                     MAVLFDYQDEYGQIPGSLSDSTIRWNFTKPPIQGLFFNKMMKKINFTKETLEEIYNYI
                     EKQVLFYIKFKDSNMDGIFEYHHGNDSGQDNSTIFKGVMVVDTPDLTAFIINAMDMLA
                     KVAEKLNKYSEKEYWANKADELTKLFLSYFIVDDLPVARETLTGKILPNNALLPLVSL
                     VLGNRLPKYTRDKMIDLIISERYLTKWGIATEAIDSDEYQDDAYWRGPIWAPTTLLFV
                     EALEECGENELAIDIAKRYCELVDKNGFAENFNAITGEGLRDKSFTWTASTFIYLAAK
                     VYEYNTQRINNIK"
     gene            complement(37000..37731)
                     /locus_tag="JD016_01655"
     CDS             complement(37000..37731)
                     /locus_tag="JD016_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAU4998334.1"
                     /translation="MGRAKYYDIYIDLKNRIENKEYLYQDLLPSEYKLIEEYSCSRNT
                     IRRAIQELANQGYVQSKHGKGVFIIYRANTQSEFLLGGTESFKESAIRNNKEYKTKVV
                     HFEEIIIDDKKSQITNFEVGTEVYYIQRVRILENKPSIIDHNYFMKDIVKDINEEIAS
                     NSIYEYMENELNETIVTTKRTMTVEKATKLDKKYLELGDYNCVAVVSNNTYNAQGIMF
                     EYTQSRHIPDGFIFFQQSQRINNIK"
     gene            complement(38173..39891)
                     /locus_tag="JD016_01660"
     CDS             complement(38173..39891)
                     /locus_tag="JD016_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HAU4998335.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLIGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDNILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(39896..40885)
                     /locus_tag="JD016_01665"
     CDS             complement(39896..40885)
                     /locus_tag="JD016_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAU4998336.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLESSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(40981..42030)
                     /locus_tag="JD016_01670"
     CDS             complement(40981..42030)
                     /locus_tag="JD016_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAU4998337.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
BASE COUNT        14296 a         7082 c         5125 g        15666 t
ORIGIN      
        1 gcatgatttt tatttaaata aatctcaaac ttcgtcttta cttacttaga tatttattta
       61 ctttctaaat cctattattt ttaaaacctt atctttttaa tacctcacca catttattct
      121 cagtaatctc tccattttcc aacgcaattt ttccatttac aattacatat tcaacaccat
      181 tggctagtaa ttgtggcttt gaaaaatcaa tatcttctga cctaggataa ctatagttat
      241 ccatgtcaat aactgctata tcagctatca acccttcttt aagaatacct ctatttttta
      301 ttttaaattg cctagctggt aatgaagtaa ctcttcttac aatctcttcc aatctaacac
      361 cagactttct gtacatattg aagaaaattg gaaaagctcc tcttctttgt agttcaaacc
      421 atgacatatc cccacccaca ctaaacaaac aatctgaacc aaccataaca aatggattat
      481 caattatttt atcatcatgt agtttatctg ggaaatcagc tctatacatt cctcctaaac
      541 aaaagataag ttcttcgtct ccatctaaaa gtaaatcgag taataacttg tcctcatcta
      601 ttcctttttg tgtagctatt tcacgtattg atttcttttc catattaata tctttagtgt
      661 ttagaagtat tatactcccc ctatctccaa gtataaatgc ttcttttaat ataagtgttc
      721 ttccctcttc atttctaaga gcatccatta ccccatctat acctaattca aatagactag
      781 atgatattgc cataataaat tgaattactc tttttttctt catacaatgc ccagaacttc
      841 ttggtactgt gtcaaccatt atttcaacac cattatacct agcctttaat atctcatcaa
      901 atgcttctat ttcaactgga cttaagtgag atacttgaac cctagcacca cttttctgcc
      961 caacctctat ggcttctttt actgcattat atcttcctat gtaatctcta atatgagatg
     1021 tatatatacc atcatgctct ttaatctgcc ttgcaatatc aacaagttca tcaatatcag
     1081 catacttact tggtatatat gccaatccag tagatattcc aaaagcacca cttttcattc
     1141 catcatttat tatatctgta atttcatttt tttccttttc tgtaggaggt ctatccttag
     1201 aacctcccat aacactccat cttatagtcc catgccccaa caagaatccc atattgacag
     1261 aaataccact tttagatata aggtcacaat attctgtaaa attattccaa aatttatttt
     1321 tatctataag atactttttt tcttcttcaa gtaatagacc atttttatac atatattcat
     1381 atacattttc agaagaatat ggtgttatag aatgtccaca attaccattt atagttgttg
     1441 taactccttg ttttagaaac ttttcaaatt taccatctag tattgctact atttcttcat
     1501 gaacatgagg gtcaataaat ccaggtgtta taacttttcc acaacaatca atctctttat
     1561 ttgtattgct atttatctta gaatctattt ttgctattaa gttgtcttta attgctatat
     1621 ctccataaaa accaggatta cctgtaccat ctattataaa tccatttttt aagattaaat
     1681 catatatcaa aatattccct ccttaaaaac caatttttaa ataatcttaa tgtaattttt
     1741 atatttgaaa atcaaattat taaatttcat atttgaaaat tcactattat ttcattgtaa
     1801 ttagtcacta agcttatctt tatacatagc tcttgcaatt aatatagtac caaatacaca
     1861 tataagaact actagagact gtggtattat accttgcttt gataatataa tagtcaatac
     1921 agatattaca attgctacta ggcctaattt tttatcttgt aaaaatactt gtccaaaaac
     1981 tgacccaaat agagctggta aaattaagtt taatttttct actacttcag ctggaatttt
     2041 agatagtacg cttcctccta aaattacccc tatagttagt atacttatat tcactaaaat
     2101 agatacagct attcctattg tagatattat cgagccttct tctgtacctg attcaacttc
     2161 tgctgccttt tgagctgcta ttgaacatgg tagcctcata ttagatatat taccagaaag
     2221 aaaactcata taagttcctg ggatacctaa gattggtgaa taagaaactg gttctattat
     2281 gtaatttgga gcactcatac tgacaattgc cattgtactt gctaataatg ctgatattgg
     2341 aggcataata ccaaatccaa agctcaaaat tattccagga actaatgctg ctataatccc
     2401 caaagatagt gttagtcttc cataccttat cataaaaggt aaatattctt cattgtatat
     2461 agattttact ttactcataa aactcccccc ttatattact gaagctatta tcattccgct
     2521 aaacattgat attgtaagcc cccactctct taaccatttt atatttttct tcttttcaag
     2581 catacacaat attaccatta tagccattcc acttatacat gcaattgttc cattatcaaa
     2641 acgcaacacc ctatcagcat ttagatatgc aaatgaaccc aacattgctc ctagagatat
     2701 tattggtata aatgatttct tgccactaga tataacattg gttatcttat ctagtttatg
     2761 tccaaatagt aatgtgaata ttatccatcc taaagaacct aatatcatag tccaaactgc
     2821 atttgtaaat gcaagttttg tcatttcagg agaacctaaa gttataccta atgcatctgt
     2881 tccaaatccg gctgacattg attcaaaaat tacagaacct ataaatgata atctcatcca
     2941 tgctataggc ccacccatag ttactataag cgataccatt ccagcaaata tagtcattga
     3001 aggcccaata gctgaaatag cactactctt aacagctgac tttatttgtt tatctgttat
     3061 tcccattgtt ttaccaactt tgcaagattt tatagcaaat ataagggatt gtaccactac
     3121 tattgatact gctactgtac tcgctatcca caaaagtgga tgattagcta attttaaata
     3181 atccatttat aaaacctccc tataaatcct caaattttat taatatttct aatatatcta
     3241 tttccaaacc tgttttagta ctctaagtat atttccacct agtaccttct ctatttcctc
     3301 atcactatat ccatacttta ctaaatatct aacaatgttt tttgaagttt ctgttgggtt
     3361 ctcaagacct tttacatatt ctactttggg atgttctttt gttcctgtta ttttttttat
     3421 atcaaattta gcagctagta ctgagtgtaa ttttacatgg tcgccatata ctgtatctgg
     3481 tccaaaagct acatggtcta ttcctactaa atttttaata tactcaaaat gttccataaa
     3541 tgactctatt gaatgttctt tattcttctc tgttattgta gtatgaggtg ctgcttctac
     3601 acctataacc cctccttttt tagcacatgc tattaatact tcatctggag ttagtctctt
     3661 tgtgttccat aaacttcttg ccccaacatg gcttaatatt attgggtcct tgctatattc
     3721 tattacatca agtgtagttt gaactccaac gtgagagcaa tctattgcca ttccaatttt
     3781 attcattctt tcaacagctt ttttcccaaa atttgtaaga cctccatctt tctcttcttt
     3841 tagtcctgaa cctaatgcat tggattcgct gtatgttata cccattaacc ttataccaaa
     3901 tccatacagt atatctattc tatcaagttc attttctatt ggtgctgcac cttctaatgt
     3961 tggtatcaat gctactttac cttcactttt agctctgtat atatcttcta ctttttcaca
     4021 ctttatgaca aaatcttgat gagctaaatc acataatctc atgcctaaat ctattaatat
     4081 atcatcccat ttccaccctg aatttgatgt tatcgtacat acaccatcca ttaaattatc
     4141 aaatatagca tcataatatc ctcttgataa tgcttcataa gcacatcttt gtttaccttc
     4201 cctattatat tcaaatatat ctctttctag gtgttctgga aataatactg gatgttcatg
     4261 taatgatata aatatttttt catccactat acttttaacc ttagcttctt gaatatcatc
     4321 taattcaata aggtattctt taactctttt atctccttta cacatctcaa tagatgaaat
     4381 atcttttcct ttttctaaat aactataggc ttcatatcct ttgtaagctg acttcataac
     4441 aacatcctcc aagttctaca attttttata tttttcctat gtagcaattg atgtgccaaa
     4501 atataaattt taattgtata ataaatcttg caaaaaataa aaacagtagt aaggttgata
     4561 tttactactg ttttattaaa aataatcttc ttagattatt tattcaatat ttttgaatta
     4621 tcttaatctt aaagataatt ttatttattt ctatccatta tagggatttt tttagggtgt
     4681 tttgcctttt tagtccccta tatcactccc ttaattttta catatgatat aaagttcttt
     4741 ccttcatcag ttataaaact accttgtttt tttactccag ataaaagtaa accatggctc
     4801 tttaacttat ctattctttt tctaatttgg tctacactta aaactatatc tttttctttt
     4861 aaaatctctt gcagtttatt tcttcctaat aatatatttt tattattcca agtttctata
     4921 ctggtcaata tacatatact ttcctcaaag aaattagatt gtttgaaatc taagataatt
     4981 gaatcgaaat tttcattaac taaagtattg tttttttcaa atctaaattg ttctggcaaa
     5041 tgctcataat ctactctatc ttcctcaact atagtatcaa tataataaat taaattttta
     5101 agctctctta catttcctgg ccattcatac aatgttaaag cttgcattga ttcctctgta
     5161 aaacatttgt tgctatttat ttcatctaaa aaatacttac ttataagagg tatatcttcc
     5221 ttacgttctc taagtctagg tatctctata tgtaatacat taatcctata atacaaatct
     5281 tctctaaagg aaccttcttt aatttttttc tttaaatctt tatttgttgc agctataatt
     5341 ctaacatcta ttggtataat tttacttccg cccattctca taacttcttt ttcctgtaat
     5401 actcttagca atctctgctg tacatctaaa gatatatcac ctatctcatc taaaaaaatt
     5461 gttcctgtgt gtgctctttc aaatataccc atttttccac cttttattgc tcctgtaaaa
     5521 cttccttcat catagccaaa aagttcactt tcaataagtg tatctgacaa agaagataga
     5581 ttaactgcaa caaatggctt gttttttctt agagattcat tatgtattgc ctgggcaaat
     5641 atttcttttc ctgttccatt ttctccaagt atcaacacag aaaagtctgt agttgctatt
     5701 tttcttgcaa tattaatttt ttcttttatt attttactct cacctacaac actctcaaat
     5761 gtatactttg atacaaaacc ttttgactga agtttatttt gaatcttgtc ctcaagcact
     5821 tgaattgcac ttatattttt aatgcttata atactcttta ttcgttcatt attttcataa
     5881 acatttactt tattaattat cagttttttg ttatttaaat ttactacttc atcattaact
     5941 tcatcttctt gaaaaaatat ttttttgaca accttatcac taaataagtc cataaagtta
     6001 ttagatataa tttcattttg gtctattcca actagattag aaaatacttt gttgcaatat
     6061 ataaattttc ctaatttatc aacagaagca ataccttcat ctataatctc caaaaaactc
     6121 ttcatggttt tattcatagt tgtataatac ctatatccac ttactgtatc ttcattgtat
     6181 ttttccttaa taatatgtaa tttatctata gatatattta gttttgtaaa gatttctata
     6241 actgtattta tatcaattac cttatcacct atgtcaatta tttgctttat gttttgtggt
     6301 atgctgtttc tgcttcccgt aataattcca atctcacatt tagacttatc acaacctgga
     6361 taatatggta ttagattaat atgaattaat cctagttttc taattatctt ggcagtttca
     6421 tctgctgact ctttataagc atctataacc ataacatcag aatcattttc tatacttatt
     6481 atctgattta tattttcaat atttatagta cgatgtacta ttacaatagg tatattttta
     6541 tcaatattat tatgtatata ttcctttata tcattgtctg aacatactac aagctcatag
     6601 cttgacagtt caatatttat cctatttaca tataccaagt tatctatatt acaaaattct
     6661 ccaaatatat tttccaattg ttttgatata tctatattta ttttgtcatc agttgataca
     6721 aataaaatat tatgtttttt catagtccca cctcctgttt aacgagtata gaaagtaata
     6781 atttggtcta ttgtagcact ttttaaaaaa gttttaaatc ttaaaaatca aattttgtac
     6841 catttactat tattttccat aataaaataa tgtaagctat cttttttcac atataattta
     6901 atttctaaaa cggttagttt gacattattg gaatataaac aaaaatttaa tataaattcg
     6961 ttaatatatt aatacatttt taagaatgat aaggaataaa ttataacaaa ttttaggaat
     7021 atataataat agggtctcag aacttagtta caagttctaa aaccctattt gtttagtgta
     7081 aaaaacatca taaaatttag cttaaatctt ttcacataat attactcttt ttctaagtga
     7141 taataaaata tatctattcc tctaaaaact gtaaaaattc ctgattaaat agtttaagct
     7201 catcatagaa tatagcatga ccactatact caaaagtata taaaattgaa tcttctattt
     7261 tttcattcat aaaaactgca aattcatacg gacatatttc atctaatttc ccatgaaata
     7321 taccagtagg cactcttaca cattttaaat cattaaataa ttcttcatct cttaaagata
     7381 cagcagttcc aatagtgcca atacctgatg cactaaaacc aatatcatta aaccatcttc
     7441 taaagctttc tggtggattt gatgcaaata ctttttcgcc aaaatcggtt accatttctg
     7501 gtctatcagt acatgcttgt gctatcaatt tatttacatc ttcacgtgtc ataccatatg
     7561 gaaattctgg aggtctttgt acaaatgatg gtgcagcagc tgcagctaat accaattttg
     7621 acactccata tccattaaat aaactcatat acctaagtac gattgctcct cccattgaaa
     7681 aaccaacaag ggtaaagtct tttaatccaa tagcatgtac caccttataa atatcgtctg
     7741 ctaattggct ataagtatat cctcctgatg ttgcatcaga tttaccaaaa cctcttaaat
     7801 caattgaaac tgttctatat ccgagttttg gcaatatatt tgtttgatat tcaaatatct
     7861 tatgtcctaa gggccaacca tgtataaaaa gtacagtttt tttagcactc ggattcaaat
     7921 catatactgc tatgtttata tcattcacat atacataata catatcaaca gcaactccct
     7981 tcaagaaaat gtatgatggt tattttatat atatgaataa aatgatgaat aattaaattt
     8041 tcaaatttat ttacaatact tctctattgc cttttcaaat aaacttacat gaagtttttc
     8101 atctaggatt attctattta atatagcttt tatataaggg tcttcaataa gacttatatc
     8161 tttataataa ttattaatag ccacatgctc caaatgcaaa tctagtttta gtctgttaca
     8221 aatagaattt ccataacata caaaaccacc attccaatat gcaccacaag aacttgtaga
     8281 acctctatat acaggatttc cacccaataa tcttatagtc tttgctaata tctccatatg
     8341 taacatttct gttatagata tatttatcca catttcactt aactctctat gcgtttcatc
     8401 taaatcaaaa ttatggtata gatattgatt tacactcgta aattcacttg atactccggc
     8461 atagtcatcc atcaataatt ctgcatagta cttatttgga cctaaaactt ttatctctgg
     8521 atatggctca tcacttgaat atccatttcg cttgtgcatg tcataatgtg catcactcat
     8581 aatatctcct cctttaaatt taatgtatga tattaattgt atgcatcaca actttttcat
     8641 ataacttaga taataaaatc tacttaaata ataaaaagcc atattaatta aaattaacat
     8701 gaccttttat ccacaaaatt actttttaat tattcatctt ttccagctac tgatagctct
     8761 ttaataacaa ctgaaggcga acctatacta ctcattggaa attctaaatc ttcccctata
     8821 acttcaatat ctttcaacag gtcaaaatag tttcctgcta ctgtaatttg ctctactgga
     8881 aatacttttt ttccttcttt gatataaaag cctttggcag ctaaagaaaa gtctcctgtt
     8941 actgaatttg cacctgaatg aagtcctgca aagctagtaa ccatcaaccc ttcatctata
     9001 tcctttataa tttcatctaa agatttatct cctttctcta tataaaaatt agttggatat
     9061 atgcttatag gtgatgaata tgaagatttt attccatttc ctgtagtctt tacacctgcc
     9121 ttattagccg tttttaagtt atgaagtaaa gttataagct ttccatttaa aacaacttct
     9181 tttttaaatg tagctactcc ttcatcatca aaatgagtag atgccatacc attttctaaa
     9241 agtgggtcat caactattgt aactatagga gatgctatca tgtctccttc tctatttttc
     9301 aataaagata atcctttttg agctgaatct gcattaaata tcccagaaaa tgtgctaagc
     9361 aaagatacca tagcttcatt aagcaatata gttttatatt tacctgatgg aatactctta
     9421 ccacctactt tagataaagc attatcaaca ccaagttttg ctatctcaca aggttttatt
     9481 tcttcaatag aatttgctat attatatcct attccatcat atttttgtcc attgtcttct
     9541 atgataggaa ctacaaaccc tataagcata ttagttttat tgcttaaatt aagaccttta
     9601 gtattagata taccattact tgatacagaa taagatatag tacatttaga taaatttacc
     9661 accttatctg aataagcttt agtctctctt tccatttcta aagctaaatc aatcaactta
     9721 tctgcctcta aattttctag ctctttagaa taagtcttta catcattata gtgcttatct
     9781 ccttcatata taaattgtac atcttcattt tcaatagtgt ttactccatc ttttacattt
     9841 tttatcaaca tatcaacagc tttatcatct aaaatttctg tataagaata acctatcttt
     9901 ccatttacct tacctctaaa agataaacca aaagatttat caagattgta tttctcaact
     9961 tctccttcat atatatttat acttaaattt tctcctgtat aataatatat ttcacactct
    10021 tcaaaacctt cacttagagc ctttttaaat aatatatctt taaaattttt aagttccaca
    10081 attatccctc ctatcttcca cctacagtaa tttcttttac ccttatcata ggttggccta
    10141 cattagttgg tatacttcct gatgatgagc cacacatccc ctgagcttgt tttacgttat
    10201 tcccaaccat atctatattc ataagtatat cgctaccttt tccaattaaa ctagcacctc
    10261 taactggctc ttgtatttct ccatttttta ttaagtaacc ttctgaaact gcaaaattaa
    10321 actctcctgt tacaggattt acagaaccac cacccatttt tttagcataa agtccatctg
    10381 gtattgactt tataatgtct tctggtctat cctctccagc agcaatataa gtgttagtca
    10441 tccttgaagt tggtgcaaat ttgtagttct gtctacgaga acttcctgta ggattcattc
    10501 ccattcgtct accattaagt ttatctatca tataagactt caaaattcca ttttcaatca
    10561 gtatattctt ttgagttgga tttccttcat catctatatt catagaaccc cagtagtttg
    10621 gtattgtacc atcatctata gctgtaatct ttgtggaagc aatctgttga cctaatttat
    10681 ctgcaaatac agagttcccc tttgcaactg atgtagcttc caaagaatgt ccacaagctt
    10741 catgaaatat tactccacca aatccattat ctattgctac catcatatta cctgcaggac
    10801 aatttttagc atgcaacata gtatgcgcta ctctagctga ttcttttgca taatattctg
    10861 ggtctatttc ttcaaatatt tcaaaccctt tacaaccccc aggaccttca aatccagttt
    10921 gattttcatt acccttagat gcaattgaac ttatccccag tctggttctt attcttctat
    10981 cctcaacata tataccttca gtgtttgcaa ttaatatttt ttggtcttta tccaaatagc
    11041 taacacttac ttgagatata tcattactaa attcttttgc actcttatat gcaaccttca
    11101 taacacttac tttatctcta tttcctattg aatttggata aatttttata ttatgtatgt
    11161 tatttatttt aatactgtca tttaatatta cactttttcc atcttccaat ttacctaaag
    11221 caacagcagc tttgtatgct acatccaaca gactatttaa tgaattgtca cttgtataag
    11281 catatatact tttcaatcct ttaaatattc ttattcctat accataattt cttccaccta
    11341 ttgcattttc tactttatta tctattaaac taatagaatt atttatagtg tcttcttcaa
    11401 aaatctcagc aaaatctcct ccagtaatta gacatttttc taatacctta gaagctatat
    11461 tttttgaaag cataaattta cctcctactt taaattttta attaactttt attcaatctt
    11521 tttacttgtt aacaaacatt atatactata ttacttaaaa taaggtaatt atgaatatat
    11581 tatacatatg ttttatacta aaatgttatc acattttatc tatacatacc catataacta
    11641 gataattaaa atgtataata ttatttgcta tagaaaataa tacactcata cataataaat
    11701 tttaggagtg aagtatatga atcaagaata tgtaaaagga aatcctattt ttaccaatat
    11761 taataaagtt ccaagacaat ttccatacct tactgatgat attgatactg atgttataat
    11821 tgttggtggt ggcgtaactg gatgtatttg tgcctactat ctagctaaaa acaatataaa
    11881 gtctgtcatt cttgaaaaag gaaggattgc acatggaagc actagcgtga caacttctct
    11941 attacagtat gaattagatg acaatttgat agatttaaca gaggttatga ctctaaaaga
    12001 tgctctaaaa gcatataacc tttgtacttc agcacttgaa gaattagata catttataga
    12061 gctatatgga aataagtgtg attatgccaa aagagatact ctactttata ctgccaacaa
    12121 acttgaagtt aaggctatca aagaagaata taacttaaga aaagaaaatg gatttgatgt
    12181 tgaatatatt gatgaatcca caaacccttt tagctttgat ttaaaatcag gattaatagc
    12241 aaagaatgga ggacgagaat tagacccgta taaatatagt caccatttga ttgatgtaag
    12301 tttaaaaaat ggtcttcaag tatatgaaaa tacagaagta aaaaaagtag atttatcaaa
    12361 tgataaggtt actgctgaag tatcgtatgg atacaaagtt catggtaaaa aattaattgt
    12421 agctacaggg tacaatacaa gtttatttac aaaaagaaat tttgcaacta agtcaaatac
    12481 ctttaatata gcaactaaac cacttaaaaa catagcttct tggaaaaaca acattttaat
    12541 tagagataac tgtgacccat ataattatct aagaactact aaagacaatc gattaattat
    12601 aggtggtgaa gatgtaagtt ttgatgatat agaaaatgaa actttagcca atgaaaagta
    12661 tgatatttta gaacaaagat tgaaaagtat gtttaaggat ataaaagaca ttgaagttga
    12721 atataaatat tgtggttgtt ttgcttcaac tttagataat ttaggtttta ttggtccaga
    12781 taataaaaat aacaatctat ggtattgctt aggttatgga gcaaatggta tactctttgc
    12841 aatattagga ggtataatgt taagtgaatt atatcttggc aaacaaaata aaaatatgaa
    12901 attatttaaa gtagatagat ttgataaata gatattatat attaaaagac tgtaaaaaga
    12961 tgaaacttta taattctaaa agttaacttt gatacagtct tttataatgt tccttttaaa
    13021 aatactttat ttataattaa atataaagta aaatgttaaa atataactga taaaatttag
    13081 tcataagata atatttaagt ttatttgatg aaatatggag gaattattat gaaggaaata
    13141 ctcgttattg ctcccactaa aggaacctat gaaaaatcaa ttcacatagt taaaaaaaac
    13201 aaatatacaa atattgatgt tgtatttggc aatctaaaag aaggtatacc tcttgctgaa
    13261 aaaagtatta atcatggtac tagaattatc ataagccgag gtggaactta caatatgtta
    13321 aaagctactt acaatatacc tatagttgaa attaaggtag atgcttatga tattataaaa
    13381 agctacaagg aggtaaaaaa ttccaacgaa ccatttggaa taattggatt taataatgtc
    13441 atctacggtt tcgatattat agaagaaata ttgaataaga aaataactat gattgaaatt
    13501 gaaaaagaag aagaaattta tgatgctata gaaaagtaca gaaagaaagg tataaacaca
    13561 tatataggag ataccactgt tgcacatatt gttaagagac ttaattgtaa gggaatatta
    13621 atcgaatcta gagaagaaaa catacttaga gccattcaac aagcagaaca aatactcgaa
    13681 gcaaccaagg atgaacaaaa aagaagatta cagatagaag ctatgacaga ttttgtacat
    13741 gatggaataa taacagttga taaaaatttt aaaataacat tatttaataa gagtgctgaa
    13801 aaaatatttg gtataaaaaa agaaaatgca ctttatcata atgttgtaga tgttatccca
    13861 aacacagttc ttccacaagt agttgagact atggaacctc aaattggtga aatacaaaat
    13921 atagggagta caaaaattat tacaaataga gttccaatca ttgttgatgg tcagattcaa
    13981 ggggctgttt caacatttca agatagcaag gaaataagtt cttttgaaca tacaataaga
    14041 aggagcttat taagcaaagg tctatctgct aaatacactt ttgatgatat aattcatact
    14101 agtgaaaaga caaaagattg tatagaactt gcaaaaaaat atgcttgtta tgatactcct
    14161 atgattatta ctggagaatc tggagttggt aaagaactat tttgtcaaag tatacataac
    14221 tatagtaagc gtaaaaatgc accttttgtt gctgtaaatt gtgctgctat acctccatct
    14281 ctaatagaga gtgagttttt tggttatgag gaaggttcat ttacaggtgc tagaaaaaaa
    14341 ggtaaagcag gtgtattcga actagcacat gaaggtacaa tcttcctaga tgaaataagt
    14401 gaaatccctc ttgaacttca aggtcgatta ttgagggtat tgcaggaaaa acaagttatg
    14461 agaattggtg gtaataaagt tattcctata gatgtaaaaa ttatctgtgc ttcaaataaa
    14521 gacttaaaaa atatgatgcg caatggttta tttagacaag acctttattt tagaataaat
    14581 atactaacat tatatcttcc ttcattaaaa gaaagaaaag aagatattat aaaattatca
    14641 gaatttttta tacataaata ttcaagcaaa tacaataaaa cgcctttaat tatcactcca
    14701 aatattaaaa aggcacttct tgaacgagaa tatgaaggta atataaggga attggaaggt
    14761 atgatggaaa gggctgttat tgtggaatca tttgactcga ttttattaga aaaggttagc
    14821 aatgaatata caaataatat agttgataaa gataatagtg attttgaatg cagtaattta
    14881 gatttaaaaa ctttagaaaa aaaatatatc tataaaattt ataacgaaaa taatagaaat
    14941 actacaaaaa cctgtgaaat tctaaagata actcgctcca ctctatggcg taagttaaaa
    15001 gaaatagaag atgatgttta aaaataatac atatctatat atatcaaaac gttttatgtt
    15061 taattttaat acataagacg tttatttgtt ttcaataaaa aatacaatta tatgataaaa
    15121 agaatctttg taagtgaaat tctcgtcaat cgtactttta attataagta atctacagca
    15181 tttcaatcga ttattaaaaa taatcatgat aactaaagat acaaactcta tatattggca
    15241 tattaattgc ttttataata cctatataaa aatgaaaggg agtgatacta gatgcacatc
    15301 ttaaaatcag tgaaaaagat accaggagga cttatggtct gtcccttgtt attgggagca
    15361 ctatttaata cagtatgtcc acaagctctt gaaattggag gattcacaac atccttattt
    15421 aaaactggtg caatgagtat acttgctcta ttttgtctat gcaatggagc tcaaatcaat
    15481 atcagacaag ctggtaaacc attgactaaa ggtatagtac ttacagcaac aaaatttata
    15541 attggtgcag tattaggtat tattgttagt aaatttattg gacctaaagg tataattggt
    15601 attacacctc ttgccatagt tgctaccatg actaattcaa atggaggtct atttgctgct
    15661 cttgcaggtg aatatggaga ctccacagat gttggagcaa tatctatctt atctctaaat
    15721 gatggtcctt tttttacaat gcttgctttt ggagtaactg gacttgcaaa tgtacctata
    15781 atagcactat ttgcagcact tataccaata ctacttggat ttatacttgg taaccttgat
    15841 gaagatatac gagaatttct tgcacctggt acaacattat taattccttt cttcgctttt
    15901 cctttaggag cagcattaaa ctttaatcaa attataactg ctggactacc tggagttgta
    15961 cttggtttag gagttactat aataacaggt atgggtgggt actttgtaat gaaacttata
    16021 cgtgcagaac atccagcagt aggtgcagct acaggtacaa cagccggaaa tgcagctggt
    16081 actcctgaag ctttagcagc tatagaccct actctagcta caatagcagc tgtttcaaca
    16141 gtacaggttg ctgctgctat tattgttaca gcaatatgtt gtccaatgtt agtttcctat
    16201 ctagataaaa gagaaaaaaa gaaaaaatct atttttaaaa caaataaaaa tattgaagta
    16261 aattaatact taaggaggta aaattaatga atgcaattat tcatgcaaat gaaaaagata
    16321 atctagttac atgtgtcaga cctttagtta aaggtgaaaa agtttcaatt gatggaaaat
    16381 cttacatagt aaatgataat ataccggtct ttcataagat ggctacagaa gatgtaaaaa
    16441 aaggtgatgt tgtatacaaa tacggtgaaa ttataggaat tgcaacagtt gacataaaaa
    16501 ctggtgacta tgttcatgta cataacattg aaagtacacg tggtcgtgga gataaaaaat
    16561 aggaggaagt taaaatgaaa ttaatgggtt atttaagaga aaatggtcag tttggtattc
    16621 gtaatcatgt tttaattatt ccaacatctg tatgttcaag tgagacagct acaagaatag
    16681 catcacttgt gcctggagca atagcaatcc ctcatcaaca tggatgttgt caaattggtt
    16741 ctgatataga gcttacagca aagacattaa taggatttgg aaaaaatcct aatgttgccg
    16801 cagttttagt agttggtctt ggatgtgatg gtattcaagc aaaagaatta gcatctgaaa
    16861 tagctacaac aggaaaaaag gtagattacg ttgttataca ggaatgtggc ggaacattaa
    16921 aaacagtttc taagggagct gaaatagtaa gtaaaatggc tcgtgaagta tcaaaagaag
    16981 taagagttga atttggtatg agtgaaatta cattagcact tgaatgtggt ggttctgacc
    17041 caacaagtgg aatagcttca aacccttcaa taggcgttgc atcaaacctt cttgtagatg
    17101 aaggtggaag tagcatacta agtgaaacta cagaagtaat tggtgcagaa catttgttgg
    17161 ctacccgttt tgaagatgaa gaaatgaaag ataagtttct taaatttgta agtgatgtag
    17221 aaaaaagagc aattgctatg ggagaagatt tacgtagtgg tcaacctact ccaggcaata
    17281 aagctggtgg actttcaaca attgaagaaa agtcacttgg gtgtatgtat aaggcaggca
    17341 ataagccatt caaaggtgct cttgaatatg ctgatattgt acctcctgat aaaaaaggat
    17401 tatattttat ggatactcca ggtcaagata tagattctat cactggtatg gtagcaggtg
    17461 gtgcacaaat tgtaatcttt tcaactggta gaggtactcc aacaggaagc cctatttcac
    17521 cagttattaa aatcactggg aatagtgata catacaacaa aatgccagac aatatcgata
    17581 taaatgcagg tagaattata acagatggtg ctaaaatagc agatataggt caagaaatat
    17641 ttaatgaaat aatagaagtc tgtaatggta aacataccaa agctgaaagt cttggtcata
    17701 gagaatttgg catttacaga atttcatcta cattttagat tgtaacaaaa tatcttttat
    17761 aaatataata cttatgtaga tacataagct aaaagttctt tctaatacta taaaaatttt
    17821 ctatactgaa aaggaactgt atcaaaatta ttttgaaaac agttcctttt ttaacctctt
    17881 ttactcaaaa tattataaaa taagatttaa acaataaacg tatcttgatt aatctctaag
    17941 aagacataaa taaagctaaa tgtaaagtgg gataaataaa atttaatatc tttgtatgaa
    18001 aagatatttg gtataatatg tctgagagaa ttcttatttg taaacaaata aaggatggag
    18061 gatgaaacaa aagaatgaac tttgaaaaca caaaaaataa aaaaataata gctactttac
    18121 tattgacact ccttttaatc gtgtcattaa catgtgtcac ttttattatg aatctaaata
    18181 aagtaacaca taatgaaaca gaacgatatc ttgtagaagt ttcacaaggt atttccacta
    18241 caattaatgt gaaatgtaaa agtatattta ggacactata ttctatggga gatacttatt
    18301 ttgagattaa tgatagtgaa aaagaagtca atagatactt atcaaaaaaa ttaaaattat
    18361 ttgaatttga ttggttaggt attatagatt ctaatggaaa ttttgtaagt gcccttaaag
    18421 atacaggtaa tgttaaagga caagactttt atagaaaagc ccttcaaggc aaagaaacaa
    18481 ttactcctgt taaatcacaa tctggtacat atggaatttt atattctgta ccactataca
    18541 atggagataa aattgttgga gctgttgctg cttggaatac tattgatact atgagaaatt
    18601 tattaagtgt agaaagcttt catggtgaag gattttccca aattatcgac caagatggaa
    18661 attttattgt aagttcagca aacaaaaatg ctccaaaaaa tgtggtaaat ttttttgaca
    18721 tgattaaaca ccagggaaaa caggttgata aaaataaact tgaagaaatg aaaataaact
    18781 taaaaaagaa caaaactggt tctatgtatt atacattaaa tgatggtatt tctaaagcaa
    18841 tgcactatct tccattacaa gaagaaaact tatacctcct ttcaattgtt ccaacaaaaa
    18901 cagcaagtgc acagtttgat ttacttctaa aaaaagcaat cttcatcaat attttaattg
    18961 ttatactatt tttattgctt attatattga ttatgtttat aaatcaaaag aacagggcac
    19021 aacttataaa aatcgcctat gtagactctg taactaaagg ttacagtaaa gcacgttttg
    19081 atattgaagc taaaaaaatt atacagtcat cttcatcaga aagctattca ttggtatcta
    19141 taaatattca gaaattcaaa cttattaatg attcttttgg tagtgaagca ggagataaaa
    19201 cattaaagta catacatgat gtcattagta attatctaga accaggagaa ttgctttgtc
    19261 gagtttctaa tgaccagttt aatgtactta ttaaaactat tcctcaaaaa gaaattctta
    19321 agtacataga aaatatcacc aatgatatta atagtttcaa taaaaatgaa gaatgtaaat
    19381 attttatagc tctatctata ggtgtatata aaattgatga cccaaatcta cctttagttt
    19441 caattcaaga ccgtgcaaat gttgcacgaa aaaataataa aggaaacatt ggaaatcatc
    19501 cactttatac atgtgttttt tatagtgatt tagagcgttt aaaaatgatt aaagaaaaag
    19561 aaatggaaaa tagaatggag gaagcattaa ataataacga atttatagta tatcttcagc
    19621 ctaaaataag cctcaaagat aatactattg taggtgcaga agcattagta agatggcaag
    19681 acccaaataa aggtcttata ccaccagacg agtttattcc tttctttgaa aaaaatggtt
    19741 ttattacaaa actcgaccac tatgtttttg aaaaagtatg cagtatgata agaaaatgga
    19801 tagataatgg agaaactcca atacctgttt cagttaattt ttcacgtgct aatttaaata
    19861 atatagattt tttagaaaaa tacagaatgc ttcgtaataa atacaacatt cctgctaagc
    19921 tcttagaaat tgaattaact gaaacacttg tatttgaaaa tctccaaaaa ttaattaata
    19981 taatagacca aatccatgaa gaaggttttc aatgttcact agatgacttt ggaagtggat
    20041 actcttcact caatatgtta aaagaaataa aagttgacac attaaaactt gaccgtgctt
    20101 tttttagctc acctaatgca gataatattg ctgaaaatca tgtaatagaa tctatagtta
    20161 atttggcaaa aaaattaaat atgaatagta tatcagaagg tgttgaaaca atcttgcaaa
    20221 tggattatct taaaaaaatt aattgtgata tgatacaagg atatgttttt tcaaaacctc
    20281 tacctcaaga aatatttgag aaaatgactt ttggaaaaaa actagaagaa accagtatgg
    20341 atagttccgt ttaaataaaa taaaaaactt gtctctataa aataatttta atcattttat
    20401 aaagacaagt ctttttattt tccaatttaa ttttattttc taaatttctt acactcggct
    20461 tgttttaaat tcccttttta agaataataa tacgattatc atagcaataa aatctgcaac
    20521 tggctgtgcc aaccaaacac catctaaatt aaattgaatt ggaagtacaa tgattaatgg
    20581 tataaataaa ataaattgtc ttaaaagagt taaaaacatt gaatgtttaa ctttacttat
    20641 acactgaaaa tacacaggtc ctacaataga aatccccatt ataggcaatg taattagatt
    20701 taggcttata cctcttactg ctatatccat caaatctgaa tctttattaa atatacctac
    20761 aaatacttct gggaaaactt gtactactaa aaatccaaat gacaatatga caactgacac
    20821 tatgatagat aatattaaag ttttctttgc acgctcatat tgttttgcac cataattgta
    20881 tgatattata gtttgcatac cttgattcaa tccaaacaca ggcatcataa acatcaataa
    20941 aatagatgta agtgctgtca tagctccaat agctaaatct ccaccataaa cctttagaga
    21001 attatttgta aacatgtgag ttatacttat agctacttcc atagcaaaag gtgtcattcc
    21061 tattgcaaat accttcttta ctatatcttt attcaatttt aagttacatt tttttagttt
    21121 taaattggac ttccctctta taaaataatg tattacccaa aagaatacta ctatttgaca
    21181 gagtactgtt gctatagctg ctccttttat acctaaatta aatacaaata taaatatagg
    21241 gtccaatatc aggtttaata cacatcctac aatcattgtc cttgctgcta attttggatt
    21301 accttctgcc ctaattgcat tattaagaga aaatgcaact aaattaaata tacttccaac
    21361 caatatgaca ctcatatagt cttttgcata aaatattgaa tctttacttg cacccaaaat
    21421 aaaaagtata tcttctaaga aaactaatcc taatatagtt ataattaaag agataattat
    21481 agataatgtc aaagcattac ctaatatctt ctctgcttct tctctatttc tttcacctaa
    21541 ctttatagac aatcttgtgc tagcacctac tgataccaac attccaaaag ctattattag
    21601 agtaaatact ggcatagtta caccaagacc tgcaatagct atagagccaa taccttccat
    21661 agaaccaata aaagctctat ctactacatt ataaagtgaa gttaccatca ttgctattat
    21721 tgcaggtatc gaatacctaa ataataaaat ccatattttt tcatgtctta atgcttcttg
    21781 attctccatg ttgtcaccat ttacaccatg tcataagtta tacaaattgg tttatttgtt
    21841 cttgggtctg ttgctatttc tgcatcaata ttaaataatt cttttagatt atccttcgtc
    21901 ataacatcat atgcatcacc ttcacaaact attttacctt tcttaatacc tatcatgtgg
    21961 tcagcaaatc ttgctgcatt attaagttca tgtattacca tgacaatagt tcttccttgt
    22021 tttttattta gttcatctaa taattttagt atctccaatt gatgtgccaa atctaaatat
    22081 gttgtaggct catccagtaa taatatatca gtttcttgtg ccaaagccat agctatccat
    22141 gccctttgtc tttgtcctcc tgataaatct tccatatttc tatccttaaa ttctgcaatt
    22201 ccagtaactt ctaaactcca atttacaatc tctctgtctt ctttactaga attaccaaat
    22261 ccacttttat gaggaaatct tccataagaa attaattctg aaacggtaag accactagga
    22321 gcttgagggc tttgtggaag tacagccata tcttttgcta tttctttaga cttctttttg
    22381 tagatgtcct ctccatttat atatattttc ccactattag ggctaataat tctacctata
    22441 gttttaagta ttgtcgattt tccacaacca ttagccccta tgatggtagt tatttttcct
    22501 tttggtatat ttaaatttaa attttttata atatctgtgt ttccatatga tacatttaga
    22561 ttcttagtat ttatacagtt cataacaaca ccctttccta atcatttacc aacattaaat
    22621 aaataaagta cggcactcct attatagaaa ctactatacc tactggtatt tctataggtg
    22681 ctagaagatt tcttgatata gtatctgcta ttaaaagtaa taatgttcct atcaatgctg
    22741 atactggtat taatctttta tgctttggtc ctattatttt tctacctata tgtggagcta
    22801 ttaaacctaa aaatgaaata ctacctgcta ctgctgtcga tacaccagat aaaattactg
    22861 catatattat aagtcttctt ctttgttttt ctacatctac accaagactt atagatactt
    22921 catcacccaa atttaaaaca tctatatatt tagatttata aatagttata cccataaaaa
    22981 tcactataaa tggaagcaca gctattacat atttccaact ggttccccaa atgcttcctg
    23041 atgtccatgc cataacccta ttaaattcct gtgttgtaaa tttcaattgg aatattgtca
    23101 aaattgaagt aaaagctatg tttacaccta taccaataag tagtaatctt gacgaattta
    23161 taccaccttt ccatgcaaga gaatatataa gaaatgctcc aaataaagct cctataagtg
    23221 caacaatagg cattgtaaat acagtcaagt tactcatacc ttcatataca ttaccattca
    23281 taaggtatat ataaactact acaaacaatg cagcacctga atttattccc aaaattccag
    23341 aatcagctaa atcattcttt gtaactcctt gaagtattac acctgctgtt gataatgcag
    23401 aaccaaccaa aagtgctatt actattcttg gtaatcttaa tttaaatatt gcaatttcat
    23461 gattacgctg accctgccct aaaaaagtct ttataacatc cattggttct atagaaaatg
    23521 agcccatatt taaactcaat aaaaaagtga taaaaataag caccattaaa attgatataa
    23581 caagtaatgt tttagaattt atttttataa tatttttcat agtacctatt tctccccctt
    23641 tctaacaagg tatataaata ttggcactcc tataagtgct gttatagaac ctactggtgt
    23701 ctcatatgga ggatttatca tcctacttaa tatatctgta tatacaagca atacagctcc
    23761 taatactaaa gagctcggta ttatatactt ataatctgaa cctactattc ctttagctat
    23821 ttgaggaact ataagaccta taaaaataat attaccagac actgacacag atgctccagt
    23881 cattaatatt acaagtacta tacatataaa tctaacaaag ttagtttttt gtcctaatcc
    23941 tattgccact tcttctccaa gacttaatat agttattctt ggagccataa taatagctaa
    24001 aataaatcca ataccaccag ctacaaatag caattttata ccttcccact tcactcctac
    24061 aagccctcct gatatccaga aacttaattg ttgagatagg ttaaaataca tagatattgc
    24121 tgatgccaaa gaaattagca atgtaccaag agcgactcct gcaagagcta attttacatt
    24181 attaactctt cctcttgatt tagaacttat aaaatatata aataatccac ttatacttgc
    24241 tccaataaat gcaaacatca ttacactaaa acttccatgt ataagttgag gaagtttttt
    24301 ttgaagtacc agtgatatcg aaattgcgaa cgtagctcct tgtgtaattc ctataacaga
    24361 tggttcagct ataggatttc tcgttattcc ttgcattatt gccccagaaa ctgctaaaaa
    24421 ccctccaaca agtgcagctg ctattgccct tggaagtcta acatccttta ctattctcat
    24481 atttattcca tcttccattt ttatcaaact atctataaca gttgatagat tcatatcttt
    24541 tgccccaata gttatggata atacaattcc aaatacaagt acaactatac ttactaatat
    24601 caacaaataa gttatatttt ttttactcag cttagtcaat tatcctacct ccttttccct
    24661 tttaatcgtc agtataatat catctattaa gtattttaat tttaagattt attaaaaata
    24721 ggtgtgaaaa atgaatatca ttatctcaca cctactaagt taagaattac tttgttaatt
    24781 catttttaac tgattctaat aataactctt ttccaattgg attgtatgat tgactgaaaa
    24841 atggagtagc atcaagtatt gttacattac catcttttac tgctcttatt tgagcccaga
    24901 tagcactatt ttctaaatct tttttatctg cttctgtagc aattacaact atatggtctg
    24961 catctatatc tgttaaacct tccatactta ccattggtag tgttattcca tcctgttttg
    25021 gcatattctt aggtctagct aatttcatat catcattgat tagagtacct attccaccat
    25081 cactaaatac cataaattga cctgaacttg ccaacactgg taaataagtt ttttctccat
    25141 tcttttctat aacttcctta cctaactttg tagctttttc atcatacttc tgtagccaac
    25201 ttttagcttc ttcttctttg tcaaataact ttgaaacatc agttaacttg cttctccagt
    25261 catttgcata atctttcatc ataactacag gagctatttc ttttaattgg tcatatattt
    25321 tttcttgtct ttgactcatt attattaaat caggatttac ttctaatata gcttccatat
    25381 ccatagtatc catcattgag tgcccaacta tctttacacc tttaagctct tctcttatgt
    25441 aacttggtaa tttatcagtt tcatatgagt ctacatttgc tgttgcaaca ggcttataac
    25501 ctgctaataa tagttcttca ctacttcctg atatatcaac tattttttta ggatttgatg
    25561 gtatcttaac ctctccttta acagattgta caactcttgt ctctgattta tctttatcct
    25621 ctgtattctt atctgaacat gctgtaacaa gtactaacgt agttataata gatataaata
    25681 ttgcaagtga ttttattttt ttcatgatta cttctcctac ttgtttatta tacttatatg
    25741 atattgatta tcataagcac atatttatta tagcattttt ctatttttag tcaatattat
    25801 tttgtaaaag gataaaaatt cctaattaat cttaattata tatagcttag ataaggtttt
    25861 cttatattaa acagtatttt ttttaagaat tggacacaaa tacaatgact tttcagttaa
    25921 acaatgataa aatgcaattt atagataagg aatttagtag ggaggattca aaacatggta
    25981 catttagtat attgtgataa tgcaggaaaa aaaggagaaa aggtcttaga taaaatcttg
    26041 gctggaacaa aaacaatggt tattagaggt gctgctggta gaaaaatacc acatagtaga
    26101 gtatttgaag atgagttact ttattttatg gaaaaaggaa ctgcaaaaat atctgcaaaa
    26161 gccactgtta aaaaggtaga aaactatgtt aaattaacag aagatgaaat ttctaaaatt
    26221 ttaaatgaaa atcaatcaaa attaaattta tctgaaaagc aaaaagttcg ttggcataaa
    26281 aaatgtttat gcttagttga atttgaaaat gttgaagaaa taacacctct tgattttgac
    26341 catcaaggaa atatggatga ttggcttatt gttgaaaaaa ttgaagatgt agttgttggt
    26401 actagtatac cttacaatta tgagaagtct aaattttaaa gtatcaataa cataatacta
    26461 tattgctaaa ctctacttaa ataaaaaaag agtgcattta ataattataa aaattatttt
    26521 tatacactct tttttaataa taattttaaa ttacttactt tctaatcttt catatacatc
    26581 tataaattct tctgctaatt gttgaaaatt catagctgtc attaaatggt cttgtgcatg
    26641 aataagtata acacttattt cagttttatt accattagct tcattttgta tcaattctgt
    26701 ttggaattta tgagctttat gaattgattc tttcgacttt tcaatacatt ctctagcttt
    26761 atctatattc ccgctctttg cttcttttat tgcttcaaat gctagccctt tcgcatcacc
    26821 tgcaaatcca attattccaa aagatatctc tatcatcttc tcatccattt attcgacctc
    26881 ctaaatatct ctatagcttg atatgactaa tccattcttt tctaaaaatt ctttgacttt
    26941 tttagatgta agtatcttgt gctctttcgt tctatcaata gcataagatg tagaatttaa
    27001 aatataatca tctaaaaaag ctgggtgaga cataatatcc actacatcat acttcattat
    27061 ttcgtctata ttttttataa aatattcttc acttacattt tccttataaa aactatctat
    27121 taaaggaact actttagaat actctaaatt atattcaaat ccacctctta ttggaagatt
    27181 atacttggtt acaatcttct ctattactga ctttaaactt actaaagtat ggatatgatg
    27241 gtgactatct aaatgagata tatctattcc aagccctttt accctatcta tttgagcaca
    27301 aagttctcta taaatctcat cacaatccat tttttcaata atttcgtctg taatcctcct
    27361 aataaaaaaa ccgtctttat caacaatagt ttttaagtta gataataatg gtctaccaca
    27421 acttaaagtc atatgaactc cacaatttaa agttttattt tctttaagta agcctactcc
    27481 atgttctacc ccaggcatat ttgccatcat agaagtagac ctaactatac cattattatg
    27541 agctgaaatt attccatagt tcacagcttc acaatatcca aaatcatctg catttattat
    27601 aattttagtc ataccataac ctactttcta tagacaacta tctattaaat ctaggaagat
    27661 aatctttatg agcttccaac atttcgtcta atatagtttt tgctaagtca tcagatggaa
    27721 tcaatgggtt tatacaaagt gccaaaagag cagattcata atcgccatat acagctgctt
    27781 tagctgccaa tacttcaaat gatttgattt gacttactaa tccatgcaca gaatctggta
    27841 aatatcctat acttattggt tttggaccat tttttgttat tacactactt acttctactg
    27901 cttgttcatc tttaaaatct cttatagaac ccttatttaa tgtattaact acttggatat
    27961 cttttttgtc attgtatata gaacttatta aattacaagc agcatcacta tagtaagccc
    28021 ctcctctttt ttcaagttga ggtggtttta catctaattt ttcatcctta tataattcaa
    28081 ataattgttc ttccaattct ttaactactt gacctctagc ttttccctct ttaaactctc
    28141 tcaactcatc ctcaagcatt tctttagttt tatagtagta tctatggtat ggacaaggta
    28201 ttgcacccaa agattttaca aattcagcat taaaatcaat tgcctttata ttttgcatac
    28261 ttatatctgc tttgacaaat ttatcaattg cttcttttgt aacatcttct ccatctaaag
    28321 atacatttag accataaacc atatgattaa gacctgcaaa atccatactt attctgtcac
    28381 tttctacatt aaatagtttg gcaacatcat tttttaaatg aattggcacg ttgcatagac
    28441 ctatatatct tctaaaatta gtatatttga atacagcctc tgttattatt cctgttggat
    28501 ttgtaaaatt caccatccat gcatttggac ataattcttc cacatctttt attatatcaa
    28561 atataactgg tattgtgcga agggctttaa ataaacctcc agcaccatta gtttcctgcc
    28621 ccataactcc atgagataaa ggaatagttt catcttttat tctcgcatct aaaagaccta
    28681 ctctaagttg tgttgtcaca aagtctgcat ctcttagagc ttctcttcta tctaaagtaa
    28741 gatttatttt catatctact ccagcttttt ttaccatcct cttagctaga tttccaacta
    28801 tctctaattt ttcttttcca gcttctatat ctacaagcca taattccttt acaggtaact
    28861 cttcatatct atttataaaa ccctctacaa gctcaggagt ataacttgaa cctcctccaa
    28921 ttgtaactat ttttagtccc tttttcatct cattatacca tccttaccta taatttaata
    28981 tactctacaa aaccccatac atttatttaa aactctatac atttatttac cgaaataaaa
    29041 ctacctagtt gttaattttc aatttattag cttccatttt ttcagacgct atgacaaatg
    29101 gaatatatat taatattgat aatattaaat taaatagtgc taaagctccg ccagtccagt
    29161 cacctgtagc cattgctcca cctaatatag gaggtgttac ccaaggtatt ttaggtaaaa
    29221 ttactggtgc tactaatcca aaatctattg ctaagtatga tacagcacta catattactg
    29281 gtgcaagtac aaatggaatc ataaatatag gatttaaaac tattggaagt ccaaatatca
    29341 atggttctcc tatgttaaat attgcaggtg gaccacctaa tactatcatc tctttacgtc
    29401 ttcttccagc tattaataaa gctactatta atcccaatac cattccagaa ccacctaaat
    29461 atacaaatgc atcaaagaat gcacctgcaa gtacatggta tccttccgtt gctcctgctt
    29521 gagccaaggc agcattttct ccacctaatt tcataagtat agtttctgta aaaggaagtg
    29581 ctatatttgc accatgaagt ccaacagtcc aaagtatatg aattataaat gcaataagca
    29641 ttgtagaacc tagactatca gctacatttg atagtggtgc tcctaaataa gtatttaaaa
    29701 tatctgttaa gaaacttcca ttagaaagca tttttataaa tattcctatt acagtaaata
    29761 tcattatagt taacattcct ggtattaact ttgcaaatga tcttgaaact gctggtggaa
    29821 ctccatctgg catttttata gttaatttct ttaccttaga taatcttaca aatatctcag
    29881 ttgataataa tcctattaat atagaagaga ataaagctgc tgtacctaag taggtttgtt
    29941 gtatcattcc ccatccttca actactgtag ttcctccctc aggaactatt ttaccaattt
    30001 gtggagccat tataaaaaag atagataaag ctataagccc agattgtaac ccatcactct
    30061 cataagattt tgctagaaaa taagcaacac ctattactaa gaatactgcc attgtaccta
    30121 ttgctcccca ccaaaggtcg cctccaaatg tggtccagtt ttctccaaat atacttgcca
    30181 tcaaattttt gtatgcttcc aatggtaagt tatttatcaa tgttcccaat gcacctacca
    30241 tagttatagg tatcattact ataaatgcat ctcttactgc tactaagtgc ctttgagctc
    30301 ctattttagc agctacaggc actatatatt tatccataaa tgacatgaat ttttccattt
    30361 tacatcctcc agtacaactt tattttgaac tatttaactc tacagctcta tctaacacag
    30421 cttttccatt acatcttcca taatccatca tatttataac ttctactgga atattatatg
    30481 gttttgccat ttcagttgct tttcctaata catatctaac ttgaggccct aaaagtaaga
    30541 catcacaatt ttctatttca ttttttaagt tcacttctga tatagcccat atttttgctt
    30601 ctataccgtt ttcctttgct gcatcctcca tttttgtaac taataaacta gttgacattc
    30661 ctgctgaaca agctaacatt atttttatca ttttttcccc tccaaattag attcattaaa
    30721 tattttttaa gtcataagtt ttttaaacta tacatttaaa atatgctttt ctattaattt
    30781 tttcttaatg tatatatctt atttttaaat gtatatacat ttaattcttg atattattat
    30841 ataccaaatg tatatacatt tcaagaaaac tttttcattt tatttaaatt ttattcgaca
    30901 acagcattca aagttaatgt atatccatag tgatatgtgt gtgagtactc aaaaatctca
    30961 ctattttgta acttacaaat ttggcttata tataaaagtg gctctggttc ttctaatttt
    31021 agattatcca tacactcttc attcgatatt acagcttgta cttctctctc tgacatggct
    31081 atcttgtatc cacacacttc ttcaacaaaa ttataaagag aattttcaca atgagtctta
    31141 tttaaatttg gaaataactt aactggcata taagtaatca tatatgaaac tcttgcatca
    31201 ttggctaatc ttactctttt tatttcccaa acattttctt ctaaacttat attcaactta
    31261 cttgataaat tttcatctgc ctgaactatt tcaagagata ccaatatatt agatacttta
    31321 tagcctttac tttccatctc tttagtaaat ccacttacag acgatatatt atcatatata
    31381 ttatttgcaa taactatact acctacacct ctttgtctag ttatatagcc ttctttaact
    31441 agattattaa gtgcagacct tacagtcatt ctcgttacat taaattcttc actcagctgt
    31501 ttttcagaag ggattaaatc cccttctttc aaactgtctg aatttattaa ctctctaaca
    31561 tgattttcta taactttata aattggctct ttcatttaat tcactcctta ttaaatcttt
    31621 tatataataa gataatttta tatacaaaca aagaaaatgt ctatacattt aagataattt
    31681 aaattttctt aatatagatt ataatttaca tatgtggaaa aatattaaaa taaggctatg
    31741 atttttacaa tcatagcctt attaataaat attaaattat aacttcaaaa ataaaattta
    31801 tttaaagagt acacataaat ttttcttaat tttaatttta aactattttt catttttaaa
    31861 taaagccatg cactcataag gtcttaaggt aattgttgaa gtaacttttc ggtctccata
    31921 attagatatt atacacttgt atttttcaag ctcttcatta tctaaaataa catcaatttc
    31981 tttatcataa aagttattta acactactaa agattctttt tcgtaacatc tcttatatgc
    32041 aaatatagaa tcattctctt ctaataatgg cttaaaatct ccatatgcta ctacatcata
    32101 cttttttctt aattctatta acttcttata ataataaaaa atagaatcct tatcttttaa
    32161 actattttct acatttatat ttctataatt atttataact tctatccatg gttttgattc
    32221 gctaaaacct gcattcttac tattattcca ttgcataggt gttcttgagt tatcacgaga
    32281 tctttcctgt aatatattgt atatctcgcc atctttcatt ccttgagatt ttaatatatc
    32341 aaaataattt aaactctcaa catctacata ttgttttata ttagtaaagt atgcattagt
    32401 catgcctatt tcttcacctt gatatacata tggtgttcct cttaataaat aaatcatagt
    32461 agctaacatt ttagccgatt ctttataata ctttttatca tctccaaacc tagatattat
    32521 acgtggttgg tcatggttac accaaaataa agcgttccat ccattacctt tttccattcc
    32581 tatttgccac tcattaagta gtttttttaa cttaataaaa tcacaatctt gcaacatcca
    32641 tttattttgg tttttataat caacttttaa atgatgaaaa ttaaaacaca tagacaattc
    32701 tttctcattt ggatttgaat attttataca gttttctata gtcgtagaag acatttctcc
    32761 tacggttaac atatcatttt ccaatactcc actttcttca cacatttctt ttaagtattg
    32821 atgtactttt ctaccatctg tataaaaatg tctaccatca ccttgtaaat catcttcata
    32881 tttctctggt ttagatatta aatttactac atcaaatcta aatcctttta tacctttatc
    32941 tatccaaaac tttagtatat tttttaattc ttttcttaca ttaggatttt cccagtttaa
    33001 gtctgcttga gttttatcaa ataaatgtaa ataatattga ttaaattttt ctacaaactc
    33061 ccaagcattg cctccaaatt ttgattccca atttgttggg gcactcttac cttctttagg
    33121 atttttaaat atataaaaat ctttataata cttatctcct ttaagtgctt ttttgaacca
    33181 ctcatgctct gtagacgtat gattaaatac gatatcaagc atgataccca tatttctttt
    33241 tttagcttct tttattaaat tttccaaatc atccatagtt ccatatatag ggtctatatt
    33301 ataataatca gctacatcat atccattatc attttgtggt gatacaaaaa atggggtaat
    33361 ccacacataa tcaacaccta aaaattctaa gtaatctaat ttttctgtta ttccatttaa
    33421 atctcctaaa ccattatttg ttgtatcatt aaacgacttt aaatatattt gatacactac
    33481 actcttttta aaatctaaca tattgtctcc tatctatcca atacatatta ataccccctg
    33541 atataaaatc aagggaatta tatttatatg ttgtcatttg ttttaaattt accactttta
    33601 taaaataagt atgttaatcc aattgttatt acaaatgtta ttgccattcc tgtaaaatat
    33661 actccccaat attgaggcat tactgaaagg aatgaaaata ctcctccaac tccaacagct
    33721 gtagctttaa cattgtgata tgatataaat gctcctccta ttgcacttgc taccataacg
    33781 gctataaatg gatatctaaa tcttaagttt ataccgtata tagctggttc tgttattcct
    33841 aaccatgctg atatagtaga tgtaatagat acacctctta agtttttatt atttttaagt
    33901 atgaaaaaca ttgctaatgt agcagctcct tgagctaaag taacagtctc tgatattggc
    33961 cacaatgtta catatcctaa agtccctgcc atttgtaagt ttactcctaa gaataaatga
    34021 tgcattcctg ttattactaa tggtggtgaa ataaatgaat ataatgctcc tgcaaaaact
    34081 ggtgctactt caaataaatg cactactcca ttagttatca tatttgcacc tgtcatagtt
    34141 ataggaccta taactacaaa agttaacatg cctgtaatta ataaagctat tggtgaaact
    34201 aatactaatt gaactgcatc tgggacagtc ttttttaaga ataattcaag tttagttaaa
    34261 atgtatgtag ctattaaaac tggtattact tgaccttgat atccaacttg agcaaccttc
    34321 ataccaaata tatcccatgt tggtgctgcg gatggatttg ccgcaaaatc atatgcagac
    34381 atcaaagctg gatgaactaa tacaagacct aaaactacac ctaacaaagg atttcctcca
    34441 aacttcttgg cagctgacca acctattaat gctggtaaaa atgtaaatga tgtgtttgct
    34501 attaaattta taatacctga aattccagct ataccagtat ttacctctat gacagactgc
    34561 tcataaaaaa ttcctggatt agctatcatg ttattaacac ccataagtaa ccctgatgca
    34621 actattgctg gtaatatagg aatgaatata tcagcaagaa ctcttataaa cttttgtaat
    34681 ttatttgact tttcattgac ttgttgttta atttcttctt tagatacttc ttctgctcct
    34741 gtttgttcta ttaaatcttt atatacttta tctactccga cacctattac tatttgaaac
    34801 tgtccacctg cattgaaaca tccttttact atatcaatgc cttctagttt attaatgttt
    34861 gccttgttag tatcttttaa tattaaacgt aatcttgtta cacagtgtgt tgcactttgt
    34921 atattttctt ttcccccaac atactcaata atttgagaaa tgctttcttt tgaatttaat
    34981 atcttattct tccccatatt catttatcct ttcttttttt catcattacc aacaagttgt
    35041 ttagacaagt acttgtttga acaactttat tgtaatattt tgcattaatt tagtcaatag
    35101 ttttttgaaa acagtttcct atttattttt ttgataaatt gactcttgat aattatttta
    35161 ttatttggta agttaaatat atacttactt gtataaacaa gttagattct atataaaata
    35221 aattatgaaa ggaatatact acaaatgaat ataaaagaaa ttccatttag ttactttggg
    35281 tcttatatgt ctatttctta ttcagatgaa cttggattta cattaaaatc actaagggga
    35341 aaatcaaaac aaaatatgga tgtactaagg attataccta ctgtaaataa catacctgta
    35401 gattatgaat ttgaggctga ttatttcagt atattaatta aattcaacaa tggtagcata
    35461 tctatatgtt ttgatggaga ttctaaaatc ttattctacg gtaagggaaa aaatgtgggg
    35521 cttaaattag atacacttcc tgtatacaat tttgaatata actatcttat ggggaaaaaa
    35581 gattcagaat attgtgtgat aaatagctac aaaaatttaa ctaagttttt agtattctct
    35641 gttgatggta aggttaattt atcacaaaat atatttatag atgactgtgc ttctaccaat
    35701 aaagctaaca acacttcaat aatagatgtt aacaataata ctgataatga atttacatgt
    35761 attatagagg atattcccac tcatatgaag gttccagatt ataaggaata taattttgaa
    35821 acttcattaa ataacacaaa aaaattattt aatgaatatt atctagctca acctaagata
    35881 gatgaaaagt ataaaaattc acttttaaga gctagttata taaattggtc ttctttcgtt
    35941 aaacctcaag gatacctaaa gagatatact atacttgctt caaactcaac attcttaggg
    36001 gcatggagtt gggatcactg ttttaacgca ttggctttag ctggtgtaga tgataaatta
    36061 gcttttgacc aaatggcagt acttttcgac taccaagatg aatatggtca aattccaggg
    36121 tctttaagtg attctactat tagatggaat tttacaaaac cacctattca gggattattc
    36181 tttaataaaa tgatgaaaaa aattaacttt actaaagaaa cattagagga aatttataat
    36241 tacatagaaa agcaagtatt gttttatata aagtttaaag attcaaacat ggatggtatt
    36301 tttgaatatc atcatggaaa tgattctggt caagataata gtacaatatt taaaggagtc
    36361 atggttgttg atacgcctga cttaacagca tttataataa atgctatgga catgttagca
    36421 aaagtagctg aaaaactaaa taaatatagt gaaaaagagt attgggctaa taaagcagat
    36481 gagcttacaa aattattctt aagttatttt atagttgatg atttacctgt agctcgtgag
    36541 actttaactg gtaaaatact tcctaataat gctttactcc ctcttgtttc tttagtgcta
    36601 ggaaatagac ttcctaaata tactagagac aaaatgatag atttaattat tagtgaaaga
    36661 tatttaacta aatggggaat cgcaacggaa gctatagata gtgatgaata tcaagatgat
    36721 gcttattgga gaggtcctat ttgggctcct actacgctat tatttgttga ggctttagaa
    36781 gaatgcggtg aaaatgaact tgcaatagat attgccaaaa gatactgtga actagttgat
    36841 aaaaatggat ttgctgaaaa tttcaatgct attacaggag aaggtcttag agataaatct
    36901 tttacctgga cagctagtac attcatatac ttagctgcta aagtttatga gtataacacc
    36961 caaagaatta ataatataaa atagagtaca aattgtactc tattttatat tattaattct
    37021 ttgggattgt tggaaaaata tgaatccatc aggtatatgt ctagactgtg tatattcaaa
    37081 cattattcct tgagcattat aagtattatt gctaacaact gcaacacaat tataatctcc
    37141 taattccaaa tattttttat ctagtttagt tgctttttca actgtcatgg ttctttttgt
    37201 tgttactata gtttcattca attcattttc catatattca tatatagaat tagatgctat
    37261 ttcttcatta atatctttta ctatatcctt cataaaataa ttatgatcta taatagatgg
    37321 tttattttct aatattctta ctctttgtat ataatacact tctgttccaa cttcaaagtt
    37381 agttatttga ctttttttat catctataat aatttcctca aaatgaacta ctttcgtttt
    37441 atattcttta ttgtttctaa tagcggattc tttaaatgat tctgttcctc ctaataaaaa
    37501 ttctgattgt gtattagccc tataaattat aaatactcct tttccatgct tactttgaac
    37561 atatccttgg tttgctaatt cctgtatagc ccttctaatt gtatttctgg aacaactata
    37621 ctcttctatt aatttgtatt ctgatggtaa taaatcttga tataaatatt ccttattttc
    37681 tattctattt tttaaatcta tatatatatc ataatatttg gctcttccca ttttaacctc
    37741 ccaattttat atataaatat ttaattgtgt cttatttcta aaaatcatgt attaatataa
    37801 tatattataa gttaagcaaa aaccaaaata aatataaatt taaaactgga actaaacttt
    37861 ttataaaaaa cttcaactgt aaaaactatt aaaacattat ttttatgcga aacttaataa
    37921 tttgtcaaac ttagcttgat aatattctaa atccaaccaa tcttccagta acaccaaaaa
    37981 ctttagattt agagaaagtt tccatagaca tctctttatt tgataaatat aattatgcat
    38041 attttcaaat gtataataaa aaaggaaaat taacactatt aatattaatt aaaatttaaa
    38101 aaagtctatt ctttaaataa taaatcacaa gaatagactt ttttaaattt gtgtttttat
    38161 aagataaatt atttaattaa agtttgttct tttttctaaa cgattcttta taaattcata
    38221 tattactttc actaatatat ttgtgactaa tattaacaca gctagtgctg ctgcctgtgc
    38281 agtactcccc ttatcatcta gttgtacaat tgatattgca gctacttttg aacttgcagt
    38341 atatataaaa actagtgctg atattgtcac catggagttt aggaaaaagt acataaagtt
    38401 ctctaaaact gctggcaaac aaagtggtat agttacgttc ttaagaattg atgcactgtt
    38461 tattcccatt gatttagcta ccatatccaa ttctttatct actttcttaa tagcagtagt
    38521 agctgttata aatgctactg aaaagaaatg aactatatta catataacca ttataagtaa
    38581 tgttccatat aatccattaa acaaattctt aatgtatatt gtatccataa aattaaactc
    38641 taatttatta aaaaataata cataagctat acctaaaact aatcctggca atgccatagg
    38701 caatgtactt aaaaaatacc caaatgattt taaatatgga gctttttctg ttttttcatt
    38761 caaatatgca aataagaaaa caaagctcgt ccctagaata gctgtcaata gtgacatttt
    38821 tattgaattt ataaatggtt taatcccatc aatactgttt ttcatagaaa aatgttccag
    38881 tgtcagtctt aaatcatatg gccaaactga tattaatgaa gataagatta cagttaacat
    38941 tacagctatt ataccaaatg caataattcc tacaacccct cctaaaatat tatctcttaa
    39001 cttattagtt ttaaccttat atggaattga ctttgaagtt atatatgaat tttgattttt
    39061 ttcaattttt ttagttacta taaacgatat tattgcaggt attaaaagta atattgatac
    39121 agttgctccc attggcatat tctgttgccc tataatttgc ttatatacat ctattgataa
    39181 cacattaaac tctcctccta tcacctttgg agctccaaaa tctgtaaaac ataatgtaaa
    39241 cgatacgaat gcagaactta ttaaagagta ttttatactt gggattgtta tgcttataaa
    39301 ttctcttatc ttgcttatcc ccatagtatt actagcttca tatagtctat aatcggttga
    39361 acttagagat atataaagta tttgaaatga ttgaggaaat acatatataa tttgtgccaa
    39421 tattattccc actgctccat atattggaac atgcactcca attaaattag ttaccaaacc
    39481 ttgatttcca aataaatata ctagacctat tgcatgcatc attgttggag caaacatagg
    39541 aaacatagct aatgatttaa aaaaaccttt aaatttaatc tttgttctag ttaatgcata
    39601 tgcatatact aatgcaagtg ttagtgatat tacagttgat ataactgata caaatactgt
    39661 attttttaga gaattaagta gagattcaga agccaagtat tcttgaaagt tttgtaatcc
    39721 tacaaagact ccatctttat ctaaaaaagc ctgtacaaat agatatatta taggtgctaa
    39781 taaaaatatt actaaagaca taactaatac tgtaagcata cattttttta tatctaaatt
    39841 catttttgat ttaagactta tacttttaga acttatagca ttattattca tatacttatg
    39901 ctcctttaac ttttggatac tttaaatagt atttttcgtc tatacttatt tttacaaggt
    39961 caccttctac taaatttaaa ttaacccact cttttataga aacatcactt attatgaaat
    40021 tttcatttaa tttatctttt atttcaactc tcaataaatt ccctctaaac tctatatttg
    40081 atattatccc ttggtagttt tcttttgtcg atttctctat ttgaacatat tctggtctta
    40141 cagttaatat agaatctcca ttatcaaaac agtttgtatc tcctataaat tgagctgtaa
    40201 ataggttttg aggattttgg tatatttctt ctggagtccc tatttgcatt atatctcctc
    40261 catttagaat agctatttta tctgccatag ttatagcttc ttcttggtca tgtgttacca
    40321 ttattgtcgt tatattaagt tctttttgta atcttttaat gtccattcta agcttgtgtc
    40381 taacctttgc atctaatgcg gacattggtt catctaaaag taaaaacttt ggttcaagag
    40441 ctaatgctct tgctattgca atcctctgtt gttgtccacc tgataatgct ttagggtatt
    40501 tatgagcttc attagtcaat ccaactgttt caagtacttc cttaacttta ttatctattt
    40561 tttcttttga aacttttctc tcttttagtg gaaaagctat attgttataa gcagtcatat
    40621 tcggaaataa tgcatatgat tgaaatacta taccaaaacc tctctttgat gactctaaat
    40681 ttgtaatatc tttatcctgg agaattatag ttccactatt tacatcttca agacctgcta
    40741 ttattctaag taatgtagtt ttaccacatc cactaggtcc gagtaaacat agaaattccc
    40801 cttcttcaat atccaaacta atattattta aaaccctttt ttggtcataa cttttaaaca
    40861 cattatttat ttttaagtaa ctcatatctt atctctcctt atatagttat ttttttattt
    40921 taaattttat atcctacttt ttcttctaaa attctttcat ctaaattatt aatttaaact
    40981 ttacttactt tcagattttg aaccgtattt tgattcccaa gttttaagta ctgtacttct
    41041 gtttttggca gcagagttta aatcaacttc ttttataagc tgttcaaatg gttgtttaga
    41101 ataacctttt ggaactggat tatctgtctt tatggaagta actgctactt catgtgcata
    41161 tctatccata gctgaatcac ttattgccca atctaaaaat agctttgctt ctttttttat
    41221 attatctttt ttaactaagg catttgcttc taagtcccaa cctgaacctt cctttggaaa
    41281 tacaacctct ataggttctc cactagataa ttgttgtgta gctctatatc caagtgtaat
    41341 acctattgga tactctccag agccagctaa cttcgcaggt tttgaacccg agtgtgtata
    41401 tgtggctata tttttatgta atttatccat gtaattgtaa gcatcttttt cacccatcat
    41461 ttgaataagc cctgatactg taagataccc cgttccagat gatgctggat ttggcattgt
    41521 tataagtcct ttatattctg gttttattaa atcttcataa ctttttggaa tatctatacc
    41581 tagtttctta agttccttat aattaactac tattgctgtc tctgggacac tcattcctac
    41641 ccaagataca tcatcttcag tatctttaaa ttttttatca actttatcat aatttttagg
    41701 cttatatgct tcaagtgcac catctttttt tagctctatc atgttcattg ctgataatcc
    41761 ccatataata tctgcttgtg gattatcttt ttctgctaat agttttgaag ctattacacc
    41821 atgagaatcc ataactatct ctacatctat attgggatac tcttctctaa aaccttccat
    41881 ataataagct atctgctctt cctctagtgc tgcatatatt gtaagtttat tatttccttt
    41941 tccttgagta gtttgtggtg ttgttgatat gcaacttgtc ataaatgtaa tcactacaat
    42001 gcacagacaa gtaaatataa attctttcat atcttccccc tttgtgtttt ttcctttttt
    42061 tatttgtttt ttattgtttt tgcttatgtt tatataatac acctaatttg ttaatataaa
    42121 gttaagtgta aattaacagt tcgttaattt ttagaaattt ttgttaaat
//
LOCUS       DACXMZ010000008        41003 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.008, whole genome shotgun sequence.
ACCESSION   DACXMZ010000008 DACXMZ010000000
VERSION     DACXMZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.008"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(185..892)
                     /locus_tag="JD016_01675"
     CDS             complement(185..892)
                     /locus_tag="JD016_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAU4998338.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYAKRLITATRVLQ"
     gene            complement(1091..3004)
                     /locus_tag="JD016_01680"
     CDS             complement(1091..3004)
                     /locus_tag="JD016_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAU4998339.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            complement(3088..4179)
                     /locus_tag="JD016_01685"
     CDS             complement(3088..4179)
                     /locus_tag="JD016_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAU4998340.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPGNVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            complement(4185..5480)
                     /locus_tag="JD016_01690"
     CDS             complement(4185..5480)
                     /locus_tag="JD016_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HAU4998341.1"
                     /translation="MKIAIAGTGYVGLVTGVCLAEVGNENVVCVDVDEQKVKSMKLGI
                     APIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVGTPERSDGSANLDYIKA
                     VAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNDVKVELASNPEFLSQGTAV
                     KDTLHASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAEMIKYASNNFLALKISF
                     MNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGSCFPKDTKALHWLSEHN
                     GYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGLTFKPDTDDIREAPSIP
                     NIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIEDAEICFIMTEWKDVLN
                     YDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            complement(5506..6258)
                     /locus_tag="JD016_01695"
     CDS             complement(5506..6258)
                     /locus_tag="JD016_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAU4998342.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            complement(6306..7214)
                     /locus_tag="JD016_01700"
     CDS             complement(6306..7214)
                     /locus_tag="JD016_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAU4998343.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            complement(7236..7985)
                     /locus_tag="JD016_01705"
     CDS             complement(7236..7985)
                     /locus_tag="JD016_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAU4998344.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            complement(8014..9204)
                     /locus_tag="JD016_01710"
     CDS             complement(8014..9204)
                     /locus_tag="JD016_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphotransferase"
                     /protein_id="HAU4998345.1"
                     /translation="MRMVFMDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDI
                     YNDNSKFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIV
                     FSHAPSADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKS
                     YNIVTASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWI
                     KFSSEKFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGK
                     RIVFLDKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYID
                     LKSEMFGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVL
                     SKIK"
     gene            complement(9204..10043)
                     /locus_tag="JD016_01715"
     CDS             complement(9204..10043)
                     /locus_tag="JD016_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAU4998346.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            complement(10046..11203)
                     /locus_tag="JD016_01720"
     CDS             complement(10046..11203)
                     /locus_tag="JD016_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HAU4998347.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            complement(11219..11941)
                     /locus_tag="JD016_01725"
     CDS             complement(11219..11941)
                     /locus_tag="JD016_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAU4998348.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            complement(11953..13026)
                     /locus_tag="JD016_01730"
     CDS             complement(11953..13026)
                     /locus_tag="JD016_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HAU4998349.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            complement(13048..14754)
                     /locus_tag="JD016_01735"
     CDS             complement(13048..14754)
                     /locus_tag="JD016_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAU4998350.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVKFKLNNLISYILNVVD
                     SI"
     gene            complement(14800..16356)
                     /gene="murJ"
                     /locus_tag="JD016_01740"
     CDS             complement(14800..16356)
                     /gene="murJ"
                     /locus_tag="JD016_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAU4998351.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            complement(16471..17526)
                     /locus_tag="JD016_01745"
     CDS             complement(16471..17526)
                     /locus_tag="JD016_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HAU4998352.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            complement(17621..18307)
                     /locus_tag="JD016_01750"
     CDS             complement(17621..18307)
                     /locus_tag="JD016_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAU4998353.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            complement(18527..20554)
                     /locus_tag="JD016_01755"
     CDS             complement(18527..20554)
                     /locus_tag="JD016_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAU4998354.1"
                     /translation="MMKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININL
                     QRKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNK
                     DPEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNE
                     YMRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYD
                     APILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGV
                     DRHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQ
                     GTYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIK
                     TFYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAE
                     TVYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGL
                     NGGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSI
                     HYNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKD
                     YLYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            complement(20805..21917)
                     /locus_tag="JD016_01760"
     CDS             complement(20805..21917)
                     /locus_tag="JD016_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HAU4998355.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTISISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKEDI
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            complement(22228..23805)
                     /locus_tag="JD016_01765"
     CDS             complement(22228..23805)
                     /locus_tag="JD016_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HAU4998356.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTTSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
     gene            complement(24102..26513)
                     /locus_tag="JD016_01770"
     CDS             complement(24102..26513)
                     /locus_tag="JD016_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HAU4998357.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            26740..27138
                     /locus_tag="JD016_01775"
     CDS             26740..27138
                     /locus_tag="JD016_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAU4998358.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIKFLFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKVFTNIIVIVLNYLFSKMIIFKEK"
     gene            complement(27174..29318)
                     /locus_tag="JD016_01780"
     CDS             complement(27174..29318)
                     /locus_tag="JD016_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HAU4998359.1"
                     /translation="MKISKKIVSLLTITFLTIMLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWTKSESAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDDRTKVELKRLGV
                     KNVYLIGGTNTLGINVEKQLKAENISFERISGNNRYQTSLKLAEKLSKEKEISNIVVV
                     NGEKGLADAVSVGSVAAQENMPIILSDTINGIKDTEKFIKEKNIKKSYIVGGIYSVSN
                     SVEQSLPNSKRIAGNNRNETNAKVIEEFYKNTDLKNVYITKDGMKKQSDLIDSLAVGV
                     LAAKNNSPVVLVGNKLDFTQNDVLNTKIIKNVVQVGGLGNENAVESIIDSQEETTYTV
                     KNTDELNVAIKKSDANDIIKFKPEKEKNITESFKVETDKSISVEFEGTYTQTISINMP
                     NGSITNLGDVNGTIKIMDVRSSTLVNKGEINYLDIYDNNGCRVENERDGDIWLVTISN
                     ESRDVHIENDGDINKISNSCSSVTIKNSGNIDTLSGSKEIAISGKKPKVDDTDKDDER
                     ASGINIKVEPCSIPKRDYVKVRIVTEPKNSNYKIYYKVVSKKPTSRYVGDKIKVSEWD
                     DAPSAGTSFEVEARNGFYIEAVEINTSTNKISKWGRSDSTDDGFKSIEVAKGLSANVF
                     IDGDKVELTTTRANSGCEIYYRIMSSKPTAMNVGSSITLSSWEKISSSSLELESSEVS
                     EKYIELVELEKSTNLVTRWGSTEKINIPNSSR"
     gene            complement(29351..30058)
                     /locus_tag="JD016_01785"
     CDS             complement(29351..30058)
                     /locus_tag="JD016_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HAU4998360.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELATNKIQYVHAP"
     gene            complement(30165..32036)
                     /locus_tag="JD016_01790"
     CDS             complement(30165..32036)
                     /locus_tag="JD016_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HAU4998361.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKSKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSRTIYVGIGDEKIDLTANPVDSTGTNLDPSTEGFRVNKIDK
                     LGIAGAKNIDDVQLAEITIKNSDLNIVSPQDLYDGYRLTVKGNMVANGTSKSISDISS
                     KDSETGKYKFTIKYTDASGKAIELTVESTSEKDLKDAKAALGGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            complement(32451..34796)
                     /gene="secA"
                     /locus_tag="JD016_01795"
     CDS             complement(32451..34796)
                     /gene="secA"
                     /locus_tag="JD016_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAU4998362.1"
                     /translation="MSVIDSILDKADEQEIKKLNIIVDKIDALEDSMKNLSDEELKDM
                     TAIFKNRLEKGETLDDILPEAFAVVREVSKRKLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYESLDMTVGVIISNQD
                     PSTRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGDGDEDLKLYELANSFIKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSRMLKK
                     QGIKHQVLNAKQHDKEAEIISKAGKLDAITIATNMAGRGTDISLGAGDKEEEQEVKDL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            complement(35061..37187)
                     /gene="slpA"
                     /locus_tag="JD016_01800"
     CDS             complement(35061..37187)
                     /gene="slpA"
                     /locus_tag="JD016_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HAU4998363.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFANVVTGSEGYTVSQKDYKKAV
                     KQIQDGIKKNIITSINIYFDGTKVSTVNVAGDQDARDKIADDFNNTVEGKLDSLGDGK
                     YVDFEISYNKGIEEYTKTELENYKKLLDNKVVIPKASGVNPGAVKEKSGSADEAVAAD
                     NDIKGSDLYNTTVEADTTNGGYKLSITAKTISDVKYGTIGAGKYPDAKVITATGTDAL
                     VKGKTVDISASYALEASTGNVSGLSLTDTNPSSDSVNVRIINAKEITIDLDASSYDSA
                     ESLAKKYVFKPSTLTSIYTDIEAGNIERVNGKYQVEIFADGKRLNTLSAVNSTIADPD
                     STAKVIIKADKLKDLKDYVDDLKTYNNGYSNSTNVSGDDRIETAIALSEKYYNSDDDN
                     ALFEKEVNSVVLVGSNAIVDGLVASPLAAEKKAPLLLTSKDKLDSNVKSEIKRVMNLK
                     NTVGVNSSKKVYLAGGVNSISKDVENELKDMGLKVTRLAGDDRYATSLEIADEIGLDN
                     DKAFVVGGTGLADAMSIAPVASKLESGEATPIVVVDGKAKTLSSDASDFLGNAQVDII
                     GGENSVSKDVERDIDDATGKSPERVSGDDRQATNAEVIKNSDYYEKGTVKNFFLAKDG
                     STKEDQLVDALAAAAVAGNFGTKFDAAGGETSPAPIVLATDSLSSDQSVAISKALGDN
                     DGENLVQVGKGIATSVVTKLKDLLNM"
     gene            complement(37399..38988)
                     /locus_tag="JD016_01805"
     CDS             complement(37399..38988)
                     /locus_tag="JD016_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HAU4998364.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGAPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNINTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYNLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVIDGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDARELANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            complement(39207..40808)
                     /locus_tag="JD016_01810"
     CDS             complement(39207..40808)
                     /locus_tag="JD016_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HAU4998365.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSNKTV
                     LEGAKNITSVGEINEKVIQQCISALKSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYVLTLKATDEWGKSTGKRVEIKVLDDKSQDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
BASE COUNT        12381 a         7572 c         4388 g        16662 t
ORIGIN      
        1 atttgattat aatatgaaat aatttattat aaaaaaaaca gcaaagaaat ttatcttctt
       61 tgctgttttt ataacgttca tcactatttt aatattacta aactcttaaa tctctatgta
      121 aaacattaaa tgtaattata tatttaatgt taatattcaa tcctatgtaa atcaatatct
      181 attattattg taaaacacgt gtagcagtta taagtctttt tgcataatta gttcctggag
      241 ccatgctagt aacttttact acatctcctg tttgtggtga atgtatgaat ttagaatctc
      301 ctacatacaa tccaacgtga tttattgaac cacctgaacc aaagaaaact aaatctcctg
      361 gttttaattc tgctcttgat actttctttc ctactgttgc ttgtgatctt gatactcttg
      421 gtaaattaac tcctgctcca tttttataga cataagaagt gaacccagaa caatcaaaag
      481 aatttggtcc agttgctccc catttatatg gcttaccttg ttgcttaaat gctaagttta
      541 aaacattttg tacctttgca tctacatttc caccttgaat agttaaataa tctccattta
      601 cccatcctgt aacattgtca acacttttta ttttaatcca tccatttgaa tgtaattcta
      661 aaacttcaac tttgtctcct gtgtttatct ttcctactac tgcatcattt gcactagctc
      721 cacttcttac atttaaaaaa tcacactggt ctactgttgc actcataggt gaattagttg
      781 gctctgctgc actagccgtt attgctcctg cttctactgt gacaactcct gctattaaca
      841 ccatagctat aaattttttc aatatatgtt ttttattttt aatattcatc ataatctcaa
      901 atacctaatc ctttcgaaaa tttaacattt tgttccttcg tttccaattc ctaattaaca
      961 ttctatctta catcaaaata aattgcaacc ttttttgtaa tctttttgta atctttttgt
     1021 aatcttttgt ttctttttag tatatatttg tttctttttg ctccccctgg ttacaaatta
     1081 gaaatatatt ctacttttct ctagttgttg caatttcttt gatattagca tcttctggtt
     1141 taatataagt tggcactaat tccctcataa taccatctat tttttcaacc tcttcattgt
     1201 tagtaatttc tcttaaaagt tttaagtatt ttgttatttt ttctctatca acatctattg
     1261 gtctaccaat aaaaatcttt ttatgctctg tatccaacag accttcttct gacataagta
     1321 gttcttcata taacttttct cctggtctaa gaccagaaaa ttcaattttt atatcaacat
     1381 ctggctcaaa tccactgaat ttaattaagt tttttgctaa atccaatatt cttacaggtt
     1441 ctcccatatc aagtacaaat atttctccac ctttagccat agccccagct tgtatgacta
     1501 agcctactgc ttctggtatt gtcataaaat atctaataat atctggatga gtaactgtca
     1561 ctggtccgcc atcttcaatt tgcttcttaa aaagtggaat tacactacca ttactaccaa
     1621 gcacatttcc aaatctaaca gcaacaaatt cagtctggct ttcagcattc atagtctgta
     1681 taatcatttc agcagctctt ttagtagctc ccattatgtt agtagggttt acagccttat
     1741 ctgtagatat aaggacaaat ttcttagctc tatattttga agacaagcca gctatgttta
     1801 aagttccaaa tatattattc tttatagctt cgccagggct cgattccata agaggaacat
     1861 gtttatgtgc tgctgcatga aataccactt ctggtttata tttattaaat atttcatcca
     1921 ttcttttttc ttctcttatt gatgctatta ctgtcttcaa atctaattta tttttatatt
     1981 ttctaagtaa ttcttgttgt attgagtaag cattattttc ataattatca actataataa
     2041 gctctttagg attaaatcct gcaatctgtc tacaaagctc tgaaccaatt gagcctcctc
     2101 ctccagttat taggattact tttccctcaa tgtaattact tatctctctt aaatttgttt
     2161 ttataggctc tctacccaac aagtcttcta tctctacttc tctaatctgt tttatatcaa
     2221 cttttccatc tataatttcg taaattcctg gtattgtctt tattttacag ttagtgtttt
     2281 tacatatatc aataatctct ttttttctac gtttctctat gtttgctatt gaaaagatta
     2341 tctcatctac attgtacttt tctaccacat cttttatatc tgaagttgtt cctacaattt
     2401 tcacattatg gattattctt ccaattttat ttttatcatc atcaattata gccactgcta
     2461 cttttttaag ttctggattt ctttttagtt cctgtataac catttctcct gcatctcctg
     2521 caccaattat aaggatattt gaaacctttc ctctagaata tgtcttgttc atgactattc
     2581 ttatagctct ataggctatt cttgcgccac ttgtaaacat tataataaat attgtattta
     2641 atacataaaa cattatagga taatcaaacc ctaataattt atttattata taaatcggaa
     2701 ttaaataagc taaagttgcc acaaaaatag atataatttc ttcttctcct gcatatctcc
     2761 ataatgtgtt gtagcactta aacaatctaa atgatactat atgaagtact atatatatac
     2821 acgctgaaat tattatatca ttagtggtaa atgcatttaa attacctgtc aagcttaaag
     2881 acattcgaaa cgctactagt acgctacaaa tatctagaat taatagtgtt aaatgtctaa
     2941 atttatattt tttaaaaagc ctattaaaaa agctttcttt ctctaaatct atactttctc
     3001 gcataactag aagccctcct ttaaacagca aaagctacgc acctaagcat agccaacaac
     3061 atctatcttt tgctgtattt tttcatttta ttttttatat ttatcaaata tagaatcata
     3121 aattttttct atatcattaa agtttaaatt aacctcataa ttttctctaa caaattgacg
     3181 ccctttctta cccatattaa gtctaagttc atcattttcc attagtttta ttatagcatt
     3241 ttttatttct tctggttgtt ttggttctac aattaagcca gtttcatttt cccatacaga
     3301 ttctggatgt cctcctacat ttgtaactat aacaggtact tcacatgctt ctgattctat
     3361 agctgccact ccaaatccct ctcttaaaga aggaaataca gttacatcaa aagaattaaa
     3421 agtcttgctt acatttcctg gagatattct tcctaaaaac tgaacatcat tttctatacc
     3481 caattcttta gctaaattta ttaggttgtc catctgactt ccagagccac ctattttaag
     3541 aattattttc ttatctttat attcatcttt aagcatctta aatgcttgta ttaaatattc
     3601 tattccatat tttttttcta aactctttat aattcctatt acaaaagcgt cttctttttc
     3661 tacttccata ggcttaaacc tatccatatc tactccaaaa aaggttacat ctacatgttt
     3721 agctgtatat ttattagcct ctttagccat atctctacta tttgaaaaga tatagtctgc
     3781 ttttctaaag ttatgcttta taatttgttt ttgtataaaa ccattattag ggaaatcata
     3841 aatatcagta ccccaaacag aaataatata tggatgatac ccaagtagac ttcctataaa
     3901 tccataacta cttgcatact gtgcatgcaa tatatctggc tttatttcat ttactagctt
     3961 ctttatctgc tttattgaac ctaagtatcc cgttttttta gaaatatttt cattttttag
     4021 ttcttctaca ttagaaccaa aattataaat ttttattcct tccatgtgac ctccatttaa
     4081 agaaattaca tgaatgtcat atcctttatt tttaaaaaaa ctacaccatt ttaaagtatg
     4141 aggatttgac ccatcagcta aataacaaac agtcttcata atatttacct tcctatcgaa
     4201 taatactcaa ttcctaactc ttccatatct ttaattttat aacaatttct tccatcaagg
     4261 actaaaggat ttttcatctt ttccttatac ttatgtatat cataatttaa tacatctttc
     4321 cattctgtca ttataaaaca tatctctgca tcttctatcg catcatctac agaatcagca
     4381 taaagtatag agtcaccaaa tattttctta gtattattta caccaattgg gtcgtataca
     4441 gtaactcttg cccctctttc gagcaaatat tttatatttg gtatagatgg tgcttctctt
     4501 atatcatcag tgtctggttt aaaagtcaaa cctaaaacag caaccttcat atcttgaaag
     4561 ctctctacta acttagatgc ctttctaact aatttaagtt tttgactttc attaacttct
     4621 attgcagctt ttactgtttt taattcataa ccattatgtt cagataacca gtgaagagct
     4681 tttgtgtctt ttggaaagca agaaccacca tatcctattc ccgcctttaa aaacttactt
     4741 cctattctat catcataact cattcctaaa gctacatctt ctatgttagc tcctgctatt
     4801 tcacaaagat ttgcaatatc attcataaat gatattttaa gagctaagaa gttatttgat
     4861 gcgtatttta tcatctcagc actatttcta tttgtaacta ttataggttg attaaaaggc
     4921 ttatagattt cctctaatat ttctctagcc cattcacttt ctacacctat aacgattctt
     4981 gaagcatgta gagtatcttt tactgctgtt ccttgtgata aaaactctgg attagatgca
     5041 agttctactt ttacatcatt ttttaggttt ctatttatat aatcttctat ctcttcattt
     5101 gtaccaattg gaactgtaga ttttattact actagagtat cttttgtcac agtttcagct
     5161 atttgctttg caactgcctt tatataatct aggttggctg aaccatcact tctctcaggt
     5221 gtacctacag ctattaatat tacatctaca tcttcataag cttttttata gtctgtagtg
     5281 taatctattc ttcctctgtt gtaattttta accataagtt cttctaggcc ttcttcatat
     5341 ataggtgcta ttccaagttt cattgattta actttttgtt catctacatc aacacagact
     5401 acattttcat tgcctacctc tgctagacaa acacctgtaa ccaaacctac atatcccgtc
     5461 ccagctatag ctattttcat ttttttacct ccaagtatat aaaacttata ctcttttttt
     5521 aatggcatta aaaacataga aacaaaatac atatagactt tttggtaaac ttaaattttc
     5581 aacatttcta taagtattcc aagttcgttt taatgctttt atcttgttac ttgatataga
     5641 atttccaact aatctgtaat tcactaaatc ttcttgaatc ccatatgcat atttttcctt
     5701 tttaagtaac tgtaaccagg ttgcagtatc ttgtcctctt cttaccaaag gcatgctgaa
     5761 ctctcctaca atctccttat caatgaccac tgtagaacat cctattatag tatttctaag
     5821 taatccttca tagtcaattt tcttaggagc tcttgcaacc ttattcgttt ttgacccatc
     5881 ttgtctcata tatctataag atgtaaaaga aaaacctaca ttttctttta acatatattc
     5941 tatctgaatc tctaattttc tactatccca taaatcatca ctatctacaa atgcaataaa
     6001 tcgaccttcc gcattcttaa taccaacatt tctagatact gcagctccag aatttttttc
     6061 aagctttata tattttattc tagcatcttc ttttcgaaaa ctttttataa tttcacttga
     6121 attgtcctta gaacaatcat ccactaataa aagctgccaa tttttataag tttgattttg
     6181 gattgacttt atcgtctctg ataaaaactc ttctgaatta taaacaggag taattatcga
     6241 tactaaaggc tcattcatat ttttactctc cttatatagc aactagcagt actataccaa
     6301 caagtttact tagatactaa ttttctttgt ccaatatatt tgtaatatcc taatgggaat
     6361 aatgctaaat acaacaattt atcagatact tttcttaatt caccaaaata atgactatca
     6421 gacaacaatg agtatctggc taaatttatg taatttttta tccacatata tggatatcta
     6481 cttaatggat atgtattttg atttattaaa tataaaaact ctaatgctct acctcttaat
     6541 gcatttttac ttaatatatt cttagtcaaa ccatcatcca aataatctct tattatcata
     6601 ggtttattta cacatataac tttatagtat ttagacattt cattccatac tacactttct
     6661 ggtaaaaatc tcacttcttt tctttctgga aaaggaaatt ttataagctc atcagttaca
     6721 aatactatgc ttttatctcc cttaacgcca tacttaaaat ataggtctgc aaagtgacat
     6781 attttcttat cttcaggcat ttctgtccct atcaaatctc cacttggata tacacaaagt
     6841 ccaacaactc cactaaatcc ttctttattc tctaaagaat cccactcatc attagcaatt
     6901 tcaagagaat ctttaattaa accatcatca ctatctacaa tgaaaaatag ctctccatct
     6961 gcatttttta cacctagatt tattgcagta tgttttcctc cattttcttt gtatatatat
     7021 cttatattta atttattttc tgatataaat ttttcaacta actctttagt actgtcgcta
     7081 cttccatcat caacaatcaa ccattcaaaa tcattgaaat cataagtttg agcttttaaa
     7141 tcattgtaca aattttcaag taaatgagct ctgttataag tcggagtaaa tacagtaaat
     7201 ttatatttat acattaatta aatcttcctc tctgtctaac tttttttgat tccgtttatt
     7261 ccataattaa taaagtagta tatagctttg tataaaggta ttttttctat atttctatat
     7321 atattccaag tccacatagc agcttttatt ttattaccac ttagggaatt gctagatttt
     7381 ctatataatg ctagaacttc atttattcca tgagcaatat gccctttttt caatatcgat
     7441 aaccataaaa cataatcttc atgtcttaca ccactcattc ttatttcaaa atccaattta
     7501 gacttatcaa tcacaacagt aagacatcct aagatatttc cttttaataa tcttctataa
     7561 tctacattag gtggaacttt tattactttg tttaattttt tatcattttc atccatcagt
     7621 tcatatgaag taaatgaaat tacatagtca ttttctaaca taaaatttac ttgtttttct
     7681 aacttactac tattccattg gtcatcacta tctaaaaaag ctataaattg tcctgttgcc
     7741 ttacttagtg ctaaatttct agcattagag acacccttat tagtctcagt ctttatacat
     7801 tttattctac tatcctgttg catataagat ttgactatat taggactatt atctgttgag
     7861 caatcatcaa taattaacat ttcccattct tgataagttt ggtttaatac tgattttatg
     7921 gttgcttcaa taaatttttc agaattatac atgggagtaa ttatagatac taaattcttc
     7981 ttcatattac cttcctcatc atttataaaa tatttattta attttactta atacacaatc
     8041 atatatcctc ttgcagttct tattatcatt atatctgaaa aacttatttc tttcattttt
     8101 ttggttatcc ataacctcaa aattattttc aataatatca ataagtttat tcacggcttc
     8161 atcattatta taagccaaag acccaaacat ttcacttttt aaatctatat atgaatcaac
     8221 tttttccata tattcatcca aatcgaattg gtaaaacaaa ataggtctat tcatatacaa
     8281 aaagtctatg gctacacttg aataatcagt tatatttgca gctgattttc tgatttcatt
     8341 tgctagagtt acccctttat ctaaaaacac aattcgtttt cctgttatat tttcttttat
     8401 atcatctata aactcatgca taagatgatg catataaatt tttacattta tatctttctc
     8461 atctaatatt ttatttaatt tgtcatcatt taaaaaattc attatatttt taaaataatc
     8521 tgtatccgta aatttttctg aactaaattt tatccaatct ctccatgtag gagtatataa
     8581 tatatctcga gtttgtgcaa cttcatttgt tggtaaattg tcatatcttg catttccaat
     8641 tatataaact gagtcatcta acattcccca atcatttacc ataacatttc tttcaaattc
     8701 agaacttgct gtaactatat tatagctttt atataggtta tctataatat ttttgaactt
     8761 tttgttcata ggttttcttt ttttaaaact tatagtccca tgatttaaaa acacttttat
     8821 cgttttttta tgaaaataat taagaacagg tacagcaaaa ttgtatggtg ctatatctgc
     8881 tgatggtgca tgtgaaaata ctatagcttt tgaattgtaa taatacagat agttttctac
     8941 acttcctctt attaattttt tgcctggaat tttatcaaat actttactgt ctttatttac
     9001 tacccaataa gtttctacat cattatgttc ttttagcata tactcataaa aaaactttga
     9061 attatcattg tatatatccc ctgaatgtcc acctataagc cagatatctc taccattaaa
     9121 cttatgctta ttaaatggat atataaaaaa agccaaaagc atatttatta aaaatttaac
     9181 cttgtccata aacaccatcc tcattataac ttgtactgtc taaatccatt aattcttcct
     9241 tttatagtgg cactatgata atctctattg gaactcaata taagacctcc tatatttctt
     9301 acaagttttg ataccataac ttttgcaaac ttataatttt ttctatagac tatttccttt
     9361 ttgcaaagcg ctccaaagcc tcttccatag ttataatctt tttgatactt ttctttagat
     9421 ttagaatgtt tttttgcagg atggtaaatt atatcatttc caaagtattt tcctttaaaa
     9481 cctaaactca ataagtttaa aacataatct atctcttcac cagcaccaaa ctctccacca
     9541 acacccaatt tctcatcaaa tcttgtatag tctttacctt caaaatcaat aaaaaaagtt
     9601 atggatgtta tagtatccaa aacgttggaa cttgtgatat cacaagttcc atcatacatt
     9661 ttcttaaatg catcaacttt atttgaatcc atagttttgc aactgtatat tttataatct
     9721 ttatttttat taaagaaatt tataactttt tctaatgtat cattttcgta gacacaatca
     9781 tcatcaggga aacctataat ttgccctaca gcattgtcta tgcctacatt tctgttataa
     9841 ctcaatcctt ttttagagct ttttatatac tttatatcta acttatcaat gtatttatca
     9901 actatttctt taactttgct attatcattt tggtctacta ctataagttc aaagtttttg
     9961 taagtttgat tttctaagct gtccataagt aagtatatat catcatatct atttaatgtt
    10021 ggcattatta atgatattaa cattactacc tcctactttt catcttccac aaatctattt
    10081 cccttttatc atttaatatc acttctttta ttagtaatac tactgcaaaa ctaaatgaag
    10141 cttctaataa taaattttct gcaaaagagt atagtaaaaa tataaataaa gctactaaat
    10201 atgctttttt atctttttct ataaagcttt ctattccttt atacatcaaa tacataaata
    10261 atatacaagc aactactcct agtacactaa ttatatatac ataagaacta tctaatggat
    10321 tagttgcttt tatagcagga ctatatgaat ttccaaaagg ttttagaaag ttcccctttt
    10381 cagccaagta tacatgccag aacttaggtc tacttgctaa taatctattt aataaagtgc
    10441 ttttatcaaa tactgtacca ataattacac taaataaagt aagtattatc ggtaatgtct
    10501 ttaggagcat tggtagtcct tttatttttt ttatatctat ccatcttatt atttctacaa
    10561 atattaatcc tgctaatata gtgaaaaatc cagttctact atatgtggtt tgataaacaa
    10621 aaaatgcaga accaaataaa attattctat cccatttatt ataatcttta tatcttaaga
    10681 atatatatgc tgcatatata gtaaccatat gataaaatac actatttgga ttaccaagtc
    10741 caaaatccat tcttgtaaca agcatatcta ttttttctat atatctatag tggacttcct
    10801 gattaaatat caatgaagga aatagattaa ataataatat agctaccatt aaaaaacatg
    10861 ccgatattat taaataattt ttaactataa atttatcatc tatttgctta gctgatacag
    10921 ctaataaaac gattaataat atagctcctt ccctatttaa taaataaagt aagacacaaa
    10981 ctccaactag taccatttct gttatacttt ttctcttgtc aaataaaaaa agtagcatta
    11041 gtattcctat tgaagaataa aataacgctg atttaatcat ccctatatcc atatttgggt
    11101 caataaatga tatcatatac tttctaagta atatgataaa caaaacaaat atataaagat
    11161 attttctaat cataatattt ctatcatcat ttaaagtctt cattttactt tctccctttt
    11221 agcttcgtaa agtattactt gcaaatattt tacaacactt ccatatctta tttacacctg
    11281 ttaagtgttt gctttctaag aaataatttc tctttttagc ccaacttttt ccccaccaat
    11341 gtatcccaaa agtgttttct gttatacatt ctggtgtaaa gtcttcttta aatccaaaag
    11401 gataaaaata ttctttgtcg taaacacgta ttccttcttc ataattattt gggtcaatct
    11461 tcttataatt cttttccata agatgagtca ttatctttgg tattgtaaac aatgaagaag
    11521 taagtacttc tttttcataa tactccaata agtcttttaa aaaaggatgt cctttttcta
    11581 cacctattat agcaccattt atatactctc tatcatcttc atacccacag attagcctat
    11641 tattttctaa aagaggagtt atatccttta atatttgcat atctgtatcc atataaactc
    11701 caccttgttc atagagtact tttattcttg tataatcaga tatgaatgcc caaagttttc
    11761 ttttataaca ttcttccaaa aacttatttc cttttatttc tttctctata tcaaagtttt
    11821 tttcattcca ctctactatc tcatattctg gaagcttttc tttccaagaa tttatacata
    11881 tattttctat atttcctttt ggaccaccaa accatacata atgtattttt ttaggaatca
    11941 aatgaacacc tcctacatat attcttgtct atcttcttct tttaactgct ctactatttt
    12001 tttgacatct tgagctttat cttttctaca aaccataact gcatcatttg tagatactac
    12061 tatcaaatca ctaataccta ttgtcgatat taatttgtca tctgaatata taattgaatt
    12121 tttagtatct atagtaatat tttcacctct tttgatatta ccttcatcat cagttggata
    12181 tattgcacct agagaatccc aagaaccaac atcattccat ccaaaatcac caggaactac
    12241 tataacatca ttacttcttt ccataatacc ataatctata gagattgatt gtatagttgg
    12301 gtatatttct tttatttttt ccatttcttc ttttgttcct aagtatttac ttatatcttc
    12361 taatttttca tatacttttg gcaagtatct cttaaaatct tctagtattt tagatacctt
    12421 ccatacgaac attccactat tccatacata ctttttagag tttacatatt ctttagcaat
    12481 ttcatagttt ggtttttcta caaactccac tacctcatat gcaacatctt ctatagtatt
    12541 ttctctatta aaatttatgt atccatatcc tgttgatgga aatgtaggag ttatcccaat
    12601 agttactagt ttatcattat tttctgctat atagatagct ttttccaaaa tagatttaaa
    12661 ttctttttca tcttttatat aatggtcagc tggatataca cacataacac catcaccata
    12721 ttttttcatt atgttaaatg ctgcaaagcc tatagctgct gctgtatttc tggcacatgg
    12781 ctctggcaat atattactat ccaaacattt atcttttact atatccttta aagcttccag
    12841 ttgcttctca ttggttacta taaataaatc atcttttttt gctagagaat ctattctgtt
    12901 tatagtttcg tttattaaag catcttctcc acttaaattt ataagctgtt taggtacttc
    12961 ttgtctacta agtggccaga aacgagttcc tcctcctcca gccattatga cattgtatac
    13021 tttcattatt tatctcccct tagcttttta tatagaatcc actacattta atatataaga
    13081 tattaaattg ttcaacttaa attttacatc ttcttgattg tctgaatttg ctccaaagta
    13141 aaatttaatt ttaggttctg tacctgaagg tcttatagct atccaagagc catcttcaag
    13201 taaaaattta agtacatctg acttaggtaa atcatcaaca ccatctttat agtctaaaat
    13261 tttatctact tttacatcag ccacattatc tattttaaca cttctaaagt aaagcatcat
    13321 ctcttttatt ttttttattc catctatgcc ttttaaagtt actgacttta agtcctcttt
    13381 gtagtatcca aatttattat atgtatctat caaagcttca tacaagttca taccttttga
    13441 agaatagtat gctgccattt cacatatcat aagtgaagat acaacaccat ctttatctct
    13501 agcatgagtt ccaattaagt aaccataact ttcctcatat cccattataa aacttctatc
    13561 attgctttct tcaaacgctt ttattttttc tcctataaat ttaaatcctg ttaaagtatt
    13621 taaacaatct acattataag cctttgctat ttttgcacct agttctgatg taactatagt
    13681 ttttattatt gtaggattgt ttttaggaag tttattttct tcaactaaac tctctaatat
    13741 atatctaact aaaagtgaac cgacttgatt tccatttaag actacatatt caccacttga
    13801 atcttttaca acgataccta ctctatcaca atcagggtct gttcctatta tcaaatcagt
    13861 gccattattc tttgccattt caatagctat attaaacact gacttttctt caggattagg
    13921 atactttact gttgaaaaat tagaatctgg caattcttgc tccttaacta ccattacatt
    13981 ttcaaaacca cattcattta agacttttct tactggctta tttcctgtac catgaatagg
    14041 tgtatatatt atttttaatt ttttcccgta ttcatctatt atattttgtc ttaaaacttg
    14101 cttcttaaca gcttttataa actcatcatc tacttcatta tctaatatag ttattaaatt
    14161 tttagataaa gcttcttcaa aatctatact tttaatagta ctgtaatcat caatcttatt
    14221 aacttctgct attatatcat ttgccatatc tatacagatt tgaccaccat ctgagtcata
    14281 tactttatat ccattgtatt ctttaggatt atgacttgct gttattacta ttcccatagc
    14341 acactttaaa tatctaacag caaaagaaag ttctggtgta ggctttaatt cttcaaaaat
    14401 ataagccttt acaccataag cacttaaagt ctttgctact tctatacaaa actccctaga
    14461 catgtatcta ctatcatgtg ctataactat tcccttttgc ttaccttctt ctccttgagt
    14521 tttcatgata taatttaaaa cgcccagtgt cgcacgtcta acagtataaa tattgattct
    14581 attagttccc gctcctatta tacctcttaa accacctgtt ccaaactcta aatttttata
    14641 aaatctatct tgtatttctt tttcatcatc ttttatactt aatagctcat ttttcgtttg
    14701 ttcatcaaaa taaggagaat ttaaccacat ctcataatta ttcttataat ccatattttc
    14761 atcctttctg actctaattt aaaatttcaa ggcaattatc tatcttctaa gtagtttttg
    14821 ttttcctttt cttaaaaatt caaaagctaa atccatctct tctaccttaa ataaaactat
    14881 caatacagta tatacagaag ctccacctaa tactccagct ccaactgata taatttcttt
    14941 aattgttcct gaacccataa atgcatatag attattatat atcaataaag tagctactgc
    15001 catcacacca gatgctacca aactttttag accagtcttt ataattttat cccctccaaa
    15061 atatccattt ttctttttaa gagatataaa taataatata actgtaatta tgttggatgt
    15121 actagttgat atagcaatac ctgcatgacc caatggtctt atcaatatta aattaagaac
    15181 tatatttatt attaaagcta tacttccatt tatcattggc gtttttgtat ctgaaagaga
    15241 gtaaaatgcc ctatataaga catctcttac tccacaagcc aataaaccta gtgaatataa
    15301 tctaagcgct atagatgtaa ggtctgttga taacgcatca aatttacccc tttggaataa
    15361 tattctaaca agaggctctg ctaatataat agctcctata gatataggaa gcaccaccaa
    15421 tataatacaa tttgaagatt ttacaatagt gcttataaat ccttctttat tatctttacc
    15481 agacattttt gctagtttag gatatataac tgttattata gatgtaacaa ataatgccat
    15541 aacaaactca tttagcctat ttgcatagtt aagagcagac aactttccat cacccaaagt
    15601 ggttgccaat aatcggtcaa caaatatatt taactgtcca acagctactc ctaccaacat
    15661 aggcaatact aggttgacta actcttttat agactcatcc tttaaattta tatatggctt
    15721 atacttgtag gacttcttaa acgcaaatgg cagttgaaac aatagctgac tcgccatagc
    15781 caataatgct cctgctggca gtatccatac atttttacct gcacttagtg ctattgctgc
    15841 tattattata atattatatg ggattcctat aagcccagga attacaaaac tgtcattcac
    15901 ctgtaaaaac gaactaaata tcttacttcc actcaggaat aaaactcctg aaatcattat
    15961 tttagtaaat aatattgctg cttggaactt agcagggtcg tttctaaatc ctgctgcaaa
    16021 tactgtaaca aattgctctg taaataaaag cccaataata gctattacta tagccattat
    16081 atagcatata tttaaaacat tattagtaaa ttttaaggca ccttcatctc cgagtctttt
    16141 ctttgtctca aagtacatag gtatataagt tgtagcaact gcagagccta tgactgctat
    16201 gattaaacca ggtatattta atgttaatac aaatacgtct gcatacatac ctgtcccata
    16261 gaaatttgca agtacaagtt ctctaaggaa acctaatact tttgaaaaca tagtggctgc
    16321 cataatccac aatgctgctt tagctgtttt tgacatcttc tttctcctta tctaatttaa
    16381 actctaaaag tagaactttt aaaactgctt ataaacatta ttctaattaa gtaaatttaa
    16441 cctatgaaaa gcttccttat aaatcaaaaa ctatttcgac tcatatctaa gtgccctaaa
    16501 tgtaatcaat agtaataata gtattaatac tatacaagat aagaatgtta gattaaatat
    16561 gctcttaaat agactataat cacctactgt atacccaact tcaactacat catttatatt
    16621 gctatctttc aagaatttat cttgtgcacg acttaagtta tcactacaaa tcagtattgg
    16681 catattttct tttgctgata ctactcccaa agaaatatat tcgtttacat cagatctact
    16741 accaaagtct aatctagcag tatagatttt ctttatatct ttttcatcat aaaacttctt
    16801 tattaaatca acatttgtac ttgacttatt tatttgcttt attccttcta tattttttaa
    16861 gttagaatca atctctgcaa taaactcttt tgaatcacct aatgcatata tttttttaag
    16921 attcttgcct tttaagaaat caatttgttc ctcaaaccct tcatctttag cccatattat
    16981 tggcattcca cttttagcgg cataagaata tacagatatg gcgttctcta atcctgttgt
    17041 tgagctaagt aaaaatgctt catcaaatcc tactgttcta tacatttctc tagctatctt
    17101 taaagatgta tcatatctgt ctttccctct tattctttct actttcattc ccattttttc
    17161 tatattacgt tcagcatctt tagatactgc ctttaaacca cctactatcg taaccttctc
    17221 tggacctagt tcttttagat aatttcttgt tactctacca agatttttac tcttagttac
    17281 aactattgga gcattttttg catatgctaa aggtgttgca cttatactat ctacaataga
    17341 ctcttcattt acaagaatta cttctttaga atccttaaat gctcttttat ttaattctgt
    17401 cgaaatctct ggtatactcc cttctatcaa ctctcttttg ggatttgcat ctgcaaatac
    17461 tcctatcgta ctagaaatag agattaacaa aactaacgat aatatagtag aaattttttt
    17521 aatcataagt tctcattcct tttctttttt gtattttttg acaataaaaa agctcttttt
    17581 aaagagcttt ttcattgatt agtaatctaa tattttaaaa ttatctagct ccgtctcctg
    17641 tcaatactac tttaacagtt ttaaagagta tcttaaagtc aagtaatata ctcctatttt
    17701 ttatatagta aatatcttct ttaagttttt cttcaggagt gatttcgtat ccaccattta
    17761 cttgtgccca acctgtaagc ccaggtttta tagcgagcct gttgataaat ccaggtattt
    17821 cctcattaaa ttgaacagta aagttcggtc tctctggtct aggacctatt aagcccatgt
    17881 ccccctttaa tatattaaac agctgtggaa tctcatcaat tctagttttc ctaataaact
    17941 ttccaacctt agttattcta ggatcatctt tttcagccca ttgtgcgcca aatttttctg
    18001 cgtctgttct catagatctc aatttatata tatagaatct tctcccgcat tttcctagtc
    18061 tttcttgctt ataagttatt ggtcctttgt cctctatttt aataagaatt ccaaatattg
    18121 ctattaaagg taaacctatc acaagtccta ttatagataa aactaaatcc ataattcttt
    18181 ggtacaagtc gaataaaact gttcctttta ctatgctata gtcaatctca cttgcaattt
    18241 ctttatctgc ccctggatag cttttaattt taggtatttc acactcatca ttagtatatc
    18301 cagacatagt tacctccata ttggaataaa aattttttta attcttaatt tcaacttatt
    18361 tctatcttat ttttgactta tttctatatt atactcaata atgagcagat tatcaataca
    18421 agaaacatca ttgtaaacaa tttgattctt tacaaaaata tatcatctct tgttatattt
    18481 tacattataa caggagatga tatgttttgc tttaatattt tctattttat tttactgtat
    18541 cttctattcc tattccaatt tgagttccca ttgtttttac tttttctgca gtatttaact
    18601 tatccatatc gctcttattg tcaataaatg cacattcaac aagtattgct ggataattat
    18661 tatttcttaa cacatatagg taatcttttc cattatcaag tgttcttgtc ttgattcctc
    18721 tatttttcat gttaaatttt tctagtattc tttttaagat atttgatgct gcagtcttag
    18781 ttgttccacc atttttatct tttactttgt aataaatttc cacaccatta ccagctccat
    18841 tagacgcatt gtaatgtata cttgtaaata aatctggctt tatagtattt gataatgctg
    18901 ttctttctcc taaagccatt gttttatctg tatctctagt cataacaaca tttatacctt
    18961 ttgaacgtaa atattcagta gttgctaatg ctgtattaag agtatatttc ttttcttgtg
    19021 ctccaccatt taatccactt gttgcccctg aatcagaacc cccatgacct gggtctatca
    19081 ctacagtttt tcctgctgct gaacctgaat tatctggttc tgttggtgca ttgtgttttg
    19141 ataaactaga tgctatagaa tttataacac tatctttcat tccatcacca atttgatata
    19201 ctgtttctgc tgttttttct gataagtttg tactatggta tgcagaaaca taagtttttg
    19261 gagttataag tattggagct tttaatttag ctgctaaagg tcctgctgtt attgaatcaa
    19321 ctattatgtc agattttgca actaacattg cactcaactc tttatctgga tagaaagtct
    19381 taattacatt tgcatttgtt tcatgtctat ctgctccaga aactctattt tttgaagtac
    19441 cattttttgc tattttagat atttggtcta taatttttga tgataaactt tgactaccac
    19501 ctatgtaata agcttcttct aaaccttgtg aagataacca attatatgta ccttgtggta
    19561 cagaatcttt attagctaat attataggtt gttggtcttc accagctttt gatgaaatat
    19621 taagagcatc atattcacca gcatatccat ttgctatgta tattttatta acatcatgat
    19681 acttatctat ttcttttgct ataagtaaag atgtttcatg tctatcaact ccaccaatac
    19741 gagtcacatt tacatttaca gcagatttaa tttgagacac tgccttttgt gaaactgaac
    19801 catcatcacc tataattatt acatcacttg gatttagacg ttttaactct gcttttgtac
    19861 tttctggtat atcatcttta tttgcaagta atattggtgc gtcatatgta gatgcaagtg
    19921 gagtagctgt aattccatct gctgctaatt ctccattaac tattacaacc ttactagacc
    19981 catctttcca accttgttta cttatctcaa cagcagtttc aaatctagta gctccagcaa
    20041 gtttttttac tggtgcagaa gtttttctca tatattcatt tactgctttt gttgaatttg
    20101 catcacttat tataaactct tgtgtaacat cataatcctt aagatttaat cccatagact
    20161 ctaagtcttc tttgtttaaa actttctgtt catctttacc atttattttt atgctttgaa
    20221 gttcccatac atttaagtat tctgggtctt tgttatagtt tattacacct tcatatgaat
    20281 ctgtcttttc attgtaattt aaagtagctt caagtggcat atcataacac ataaaattta
    20341 atgttattga atcagcattt aatttttctt taaattttac acttgcatta gatttacttc
    20401 ctaacactac actttttctt tgtaaattta tattaatatc tgaattagat tctgtatttg
    20461 cagtagtatt ttcagccgct aaagctacgt ttggtgccac cattgctaca gacaacattc
    20521 ctgttgccaa taattttgtt gtttttttca tcatatcccc cctaattttt tggttcttta
    20581 actctttaaa tcttttttaa tacatcttaa gcttgtttta tattacataa attcaatatt
    20641 atgtaaatct tttttcatta tagcacaaaa aattacaaat tgtaaacttt ttgtaatctt
    20701 ttatggcatg gtttttaaat tttatcgagg agaaattttg attatatttg atagtttccg
    20761 tatatttgaa caaatcaaag actttatcat ctaaaaatat tgcactaaat tttaataaat
    20821 atttttttct tgtacattat agacattact ataacccaga ataggacata caataaagaa
    20881 aatataagag aatcccaagt tgtacctgcc caaggtgtta taagttcata tgtagtccac
    20941 tctaccaagg tagttggata atcgactttg ttcgtaagtt ttggaacatt ccaaaaaaca
    21001 tgactcaata tttcaaggca catatatgta aaaatagggc tacttccaag tgctattatt
    21061 ggggtaaata ttttactttt atttttgatg tcgcatataa aataaaatat tgacagtaat
    21121 ataccataac tgccagccat taaaagtaca aatgagctag accataatct tttattaaat
    21181 ggaaaatact gattaaagat aaaagcacct attagcagga ttatgctcat aaccagtatt
    21241 ttaaagaatt ttttatattc tcctatgtct tcttttaaaa gtacacatcc catagtgcat
    21301 cccaacatac ctgaagaaat tgctactata ctagttaaaa aaccatctgg ttcaaattcc
    21361 ggcttgtaca aatgtccttt gaagaaatgt aaatccacta actgtgcaag agaaccttct
    21421 agctcaaatc cataaggttt tgctaatatg tagtacccaa ctattataaa tgtagatata
    21481 catagaaaag taattattgt agcagtactt ccaacattta atttttttag tagtaaatat
    21541 actaaactag tcacaaaata gactaatccc atacgttgaa gtactccaag tattcttaca
    21601 gtatctaaat caggatttcc taggtaattt aagaaaaatc caaataatat caataagata
    21661 cttctcttga atatactaag tattatactc agtgtggatt tattattttt tagtttacta
    21721 tttattgata ttgaaattgt aacacctaaa gatataacaa aaaaaggaaa tgcaaaatct
    21781 gcaagtgtta caccatgcca tacagcatgt cttaattgag gatacattct catccaagtc
    21841 ccaggattat tgcagactat catgagggct attgatagcc ctcttattat atctattgat
    21901 ttaatcctac tattcataag tttagaatct acagcattat ttgaaatttt attgctattg
    21961 ctattgctat tgctattgct attgctattg ctattgctat tgctattgct attgctattt
    22021 agattatttt tctttattct agttttgtca atacttttat ttattttttt gttacattca
    22081 attccaatcc ccatgttacc tccccaaagc ttattcaaat atatactaaa ctacatattt
    22141 atctataaat ctatttgtat ataaacctgt ttaactacat aaatttagaa caattacaac
    22201 acacacttca cttaaatata catattctta gctaagaaat ttctttagaa atccaagctt
    22261 atctagtata tcttgtattt tattgacttt ttgacttata gaatctatag ctttttcaat
    22321 tgaatctact ttagcctcta aatcttcaac ctgattaact tttttatcta tagtaccatt
    22381 attagttata tcactattag atgaattatc tataagtttt cctatatatc catcattaat
    22441 caattttgta tcattagcag aagctaatat catcaacgta tctatatctg aatttctagt
    22501 attttctata actctaccat ttttgtcacg aactgacaat atagtgattg tatcatgatt
    22561 attaaatcca ccttctccaa tatttctaac tataacattt accaaagtag catagttatt
    22621 cacttcacca ttaggcatat ctactgtaag agtctttgaa caatcaccct ttatattcac
    22681 agttattggt ttatttgtta ccattctaaa agcttcattt acagtattct cttttggttt
    22741 aaagtttata atatcattag gacttgctat atttatcata tcagttaatt cctcaacagt
    22801 ttttgcttca aacacagttt tttcttgtag acttttaatt tcatcaaaag cttcttcatt
    22861 tccatttcca cctacctgtg ttatagtatt taatttttta gtactaagta tatctctttg
    22921 ttttgtgttt aatttattac caaccaaaac aacaggagac tcattttttg cagccaatac
    22981 tccgacagct aacgagtcta ttagttgatt ctcattttta ctaccatcct tagttacata
    23041 taaattactt aaatttgtat cagtgtaaaa cttttctata actttagcat tagtctcatt
    23101 tctatctttc ccactcactc tttctgcatt aggaagactc ttttctacag ctcttgaaac
    23161 tgaagttgta cctccaatta catatgcttt tataactttc tcatccctta taaatttatc
    23221 aaatgcttct actccatcct taggactaga caaaattata ggcatcttat tttgagctgc
    23281 tggtgctcca acactaacag catcactcaa tcctttttca ccatttacta cagcaacttc
    23341 cttaatatct tttatatcct taagcttatt agccaaaatc agtgaagttt catatctatc
    23401 cttaccatta attctttcta cattaagtcc attacctttt aacttgttct ctatatcttt
    23461 attaagtaca gcagtacctc ctatcaaata aatatcttta gctcctagac gttttatctc
    23521 tttttcagtc ctatcatcta gcttatcact ttctgtcaaa agtattggtg ctcctttagc
    23581 ctttgcaaat ggtgtggctg aaagtgcatc agctaatgaa ttgtcattaa ctaacactac
    23641 attagaacta gaactgtatg ccttttgact tatctttaca gcagtttcat atctaccatt
    23701 acctattaat ttttcctctg taagtgcata tgatggtatt atagaaatgc tacttaaagt
    23761 aagcgctatt gtcagtattg ttaaaatagt ttttggtgct ttcattatta ttccccctta
    23821 ttttgtttaa aatcttacta tatttatatt ataaaataac tttacactat tttacagttt
    23881 aaaattatta catttttcat caataataag aaaactatct aaaaatatag acaatcctat
    23941 ttttcattat ttaaatgata aaaatataaa acaacaaaaa agagtgcctc atgaactaaa
    24001 aagttcattt tgagacaccc ctttttgtta ttttatattt ataaacttct attgaatatt
    24061 ttttaataat aaaatgtata tccttactat ctttccagtc actattttcc taaaagagta
    24121 tttagttcat taaaagcatt ttcatttcca tttccaccaa ctctaatagg agtttctatt
    24181 ttcttagact taagtacatt tttttgactt tcatctaact tatttccaac taaaactact
    24241 ggtgaatcag ttttagcacc caatgctcct actgctagtc catctatcaa gtctccttga
    24301 ctctttgaac catctttaac aacaaaagca tatttcaaat ctgaactagg gtagaattta
    24361 tctattatct tagcattagt ttcatttcta ttacttcctg caagtcttgt tggatttgaa
    24421 agcttacttt ctaaatttga tgataaagta gctgttccac caattatata agacttagtt
    24481 atttttgatg aatttaaaaa ctcatcagca ccttgcaact cacttttttc attagtaagt
    24541 attattggca tattgttatc agcagctgct gcaccaacac ttattgcatc tgcaagtcca
    24601 tttacaccat ttgccactgc aacttgtgaa acagatttta tactatttaa cttttgagct
    24661 agagatatac tggtcttgta tctatcacta cctgaaattc tttctactga tatacccata
    24721 ttactaagtt gagtttgtac attcttgctt aaagaatttt ctccacctat tagataaact
    24781 tttttagttc ctaatctttg taactctgcc ttagtttttg aatctaagtt atcttttcct
    24841 gtcaaaagaa ttggagagtc gattgctgct gtaaatggtg tagctgttaa agcatccact
    24901 attgcgtctc catttactaa tactgcattt tgtgaagaag tccatccttt ttgactaact
    24961 ttaactgctg tttcatatct atctgctcca gttaaagatt ctcctgaagt agcttgtcca
    25021 gaagatgttt ttgttatagc ttttataaca aagttacaag gagaaaattc actatatgta
    25081 tttatatctt caaatttatt gttttgaagt atgtagcttt cacctaagtt tgctttagcc
    25141 tcatataaat aatatgcatc tatattagtt tcatatgcta aagttgattt ttctctatta
    25201 ggatttgctg tgttgtctat aactactact attgctcctt taccttttgg taagctgtaa
    25261 gaattagtag gtacatttat gtatccagaa tatgatactg ttccacttgc aagttttgtc
    25321 attgaattgt tttgaggaac tccatttact actggtgcat aatatacttc atattttgct
    25381 cctacagaat ctgtttcaaa cataacagag tcaagttttt cagaatctct gctaaaatca
    25441 aatacatttg ctgctgttac tttatttgac attatcttgc taagaccagc atattcaagt
    25501 tggtacattt ttttatcact atctggtttt gatactgatt tcattgcgta gttatctgta
    25561 tctgttagta gagttttatc ttcataagaa atccagaaga atcctttcat agaattaaac
    25621 tctccccaac tactttttac taaccatgct ccatttgatt ctggttttac atcagatgca
    25681 aaattatctt ttgaataatt atcatcccat cctactatcg ctacagcgtg atttaatgga
    25741 gctctcttat ttgtacagtt atatgctgtt tcatctttat taaaatatgt tgaataatgt
    25801 gcataacctg atgtcactga accatattgc attatagcat tctttacagt ttccttatct
    25861 ttattaagac gaacaacatc tgttacatta aattgagtag gtgcagtatc catatttgaa
    25921 ggtttagttg caccttgtgc ctcagattta agattatatg gtatgtcctt ttctaactta
    25981 ggacctgccc atgctgtcag atatcctata gctgttacat ttgaactccc tgacatatca
    26041 tctaagttcc atccatattt tccaccagtt gcccaccatc ttaaatgttc ttctgataag
    26101 tcatacgttc catatccttt taatttaaga tatgcttcta aagttgacat acctgaaaaa
    26161 gaccagcatg tattaagact tccttgattt tttgctggtg tagtaagacc aagatccatt
    26221 gggttgtatg ctacacttga tttatatgct gatggaagac tacttctacc cttatttcga
    26281 agtgttgtac cttccatttc atgtggaaaa ggtattactc cattatattt tgctgtgtct
    26341 ccatttttga catcttctag gtattgaaga tagctttcag aatactctgc agtttctact
    26401 ccatctagag ttttatggtt ttctgctgag acaggtattg ttgacactat taaaaagcaa
    26461 actgtcaaca atgctagtag ctttgacaat ttttttgatt tgtactttct catgtttacc
    26521 cccttatatg tatttaattt ttattatatc agatttctaa attaaggtca tattttattt
    26581 tgataaaata tggattatga gtatatatta attatagaat attatagttt tatttttata
    26641 atattttaag atatattgat ttattaatag atgtgctata ctaatgtttg ttagtatctt
    26701 taagtatatt tatattaaat tagcttagga ggattatatt tgattttaaa aaaacataga
    26761 gagacaatat tatatttatt ttttggagca tttactactc ttgtaaatat agtttcgtat
    26821 ttatttttta ctagagttat tttatttaat tttatggttg caaatgcttt ggcatggata
    26881 cttgctgttt tatttgctta tgttactaat aagttttttg tgtttgaaag taaaagaatt
    26941 gaaataaagt tcttatttaa ggaatttttg tcttttgtta gttttaggct actttctggt
    27001 gtcgttgaaa tgttaattat gtatgtaatg attgatttac tttttgtaaa tgatgttata
    27061 gtgaaagtct ttactaatat aattgttata gttttaaatt atttgtttag taaaatgata
    27121 atttttaaag aaaaataaag tagcctacaa atatgttaga ctactttatt tttttatcta
    27181 ctagaatttg gtatatttat tttttcagtt gaaccccatc tagttactaa attagtagat
    27241 ttttctagtt ctactaattc aatatatttt tcacttactt cagaagactc taattctaaa
    27301 ctactggaac taattttttc ccaactagat aaagtaatac tacttccaac attcattgct
    27361 gttggtttac tagacataat tctatagtaa atttcacatc cagaatttgc tcttgttgtt
    27421 gttagttcaa ccttatcacc atctataaat acatttgcac ttaaaccttt tgccacctct
    27481 attgatttaa aaccatcatc tgttgaatct gacctacccc atttagaaat tttatttgtc
    27541 gaagtattta tttcaacagc ttctatataa aacccatttc ttgcttcaac ttcaaatgat
    27601 gttcctgcag aaggtgcatc atcccattca cttactttta tcttatctcc aacatatcta
    27661 gatgtgggtt ttttggacac tactttatag tagattttat aatttgaatt tttaggctca
    27721 gtgactattc taacttttac ataatctctc ttaggtatag aacaaggctc aactttaata
    27781 tttataccac tagctctttc atcatcttta tctgtatcat caacttttgg tttttttcca
    27841 cttatcgcta tctctttact tccactcaaa gtatcaatat ttcctgaatt ttttattgtt
    27901 acactagaac aactattaga tattttattt atatctccat cattttctat atgcacatct
    27961 ctggattcat tagatatagt tactaaccat atatctccat ctctttcatt ttcaactctg
    28021 catccattat tatcataaat atcaagataa tttatttcac ccttattaac taatgtacta
    28081 gatcttacat ccattatttt tatggtacca tttacatctc caagattagt tatgctacca
    28141 tttggcatat ttatacttat agtttgagta tatgtccctt caaactctac acttatagat
    28201 ttgtctgttt ctactttaaa agattcagta atattttttt ctttttcagg cttaaacttt
    28261 ataatatcat tagcatctga ttttttgatg gctacattta attcatcagt atttttaact
    28321 gtgtatgtag tttcttcttg cgaatcaatt atactttcca cagcattttc atttcctaat
    28381 ccaccaactt gaacaacatt tttgataatt ttagtattta atacatcatt ctgagtaaag
    28441 tcaagtttgt ttcctactaa aacaactggc gaattattct tagctgccaa aactcctact
    28501 gccaaagagt ctattaagtc actttgcttt ttcataccat ctttagttat atatacattt
    28561 ttcaaatctg tatttttata aaactcttct ataacttttg catttgtttc gtttctatta
    28621 tttccagcta ttcttttaga attagggaga ctttgttcta cagaattaga aacagaatat
    28681 atccccccta ctatatatga tttttttata tttttttctt ttataaattt ttctgtatct
    28741 tttatcccat ttattgtatc agaaagtata atcggcatat tttcttgcgc agctacagaa
    28801 cctacactta ctgcatctgc taaacctttt tctccattaa ctacaactat attagaaatt
    28861 tccttttctt tacttaattt ctcagctaac tttaaagaag tttgatatcg attatttcct
    28921 gatattctct caaaacttat attttccgct tttaattgct tttctacatt aatacctaat
    28981 gtatttgttc ctcctatcaa atatacattt tttacaccta acctttttaa ttctactttc
    29041 gttctatcat ctaatttatt actttgagtt aataatatcg gcgcatcttt tgcctttgca
    29101 aatggagtag ctgataaagc atctgctatt gaattatcat ttactaatac agcactttca
    29161 gactttgtcc atccagcttg acttattttt attgctgttt cccatcttcc agaacctgtt
    29221 aatgtatctt ttgtagatgc attagatgta tttccatata acataatagt taaaaatgtt
    29281 atagttaaca aggacactat cttttttgat attttcatta atttttacct cctaattctt
    29341 tcataatatt ttatggagca tgtacatact gtattttatt tgtagctagc tcatcaaaat
    29401 gagattttac tgaatggtat cctatagcat aatgtccact ttcaggattc cattttttat
    29461 atgcattcat tgcacttaat aatttctctt cttgactagg taataccttc catacctcta
    29521 catttggatt aactttttta aaatccttat agtcatatgg ttctacataa aatcttaaat
    29581 cttttatttt gccctcttta tataactcta gtgcagcttc tcctaatgct ctatggtctt
    29641 catgtattcc actagcttta aacggagtaa cagtttttac ttttgcatct ggatattctt
    29701 ctaagtactt aagtattatt tcccttgcct tttctttact taactcacca tcatgggctc
    29761 tattatcttc tatatgtata ttgctttctt ttacacccat tgcttcacaa ctatccttaa
    29821 attcttcatc tctatctttt gaaaattcac tttctgataa agagtactta tgaacatctt
    29881 taatatgata agaacatgac cctccatcat ccaaaacttt tcttattata gatttactac
    29941 catctgtgca taatatgaca tgtgtatcac ttttttcaac atgttcaatt atagcacttc
    30001 ccatacttaa ggtttcatca tcttgatgtg gtgtaaaaaa taaagttgca tctaccacta
    30061 ctacatcctc ctactttata aaatgttata atttacaata aatagcataa ttatagaaaa
    30121 agattcctgt attaattaag gaatcttctc cacagtataa attattacca acctaatatt
    30181 ttagctatct tctgtattac tgtcaattca actccattac ctatttgagt tacttctgca
    30241 ccaagattta attttttact aagtgcattc ttttgtgagt ctgttatatc agttttagct
    30301 agaagtattg gacctttacc tgctgctaga ggtcctgctg ctaatgcatc tactagcata
    30361 ccattatttc catatccatc ttttgctata tatagtttat ctaatttgcc ataatatgtt
    30421 tctataactt tagcattagt atcgtgtctt gtttctcctt caactctttg aacttttcta
    30481 tctttatcaa tatctgcaag ttgattttca atttggctag atacattatt agagccacca
    30541 actatgccaa tctcttttcc atccatcaat ttgatagcat ctgctgatag atcattccaa
    30601 ccatttacta ttataggagc cttaagttca gcagctttag cagctatact catagcatca
    30661 gcctcccctt ttgcgcctac tacaaatgca tctttaaaag accccatagc ttttgctact
    30721 gctacagaag tcatatgtct atcatctcca gctagtcttt caacattctt agttactgat
    30781 tctaattgct ttttagctgt atttgatact gctgattctc cacctactat atatatttta
    30841 gctgatgggc ttttcttaat tatatctttt atataatcta aagttacttt tggtatttca
    30901 ttatcagatg ctaataatat aggtgctttt ttagattgag caagtggtgt agctgctaat
    30961 ccatcaacta gtttatttga attaactata actatattgt cagtatattt tgtttgtttt
    31021 gctatagcca ctgcagttgc atatctatca tctccagcta tcaatttaac ctttgaatta
    31081 cctcctaatg cagctttggc atcttttaaa tctttttcac tcgtactctc tacagtaagc
    31141 tctattgctt ttccagatgc atcagtatac ttaatagtaa atttatattt tcctgtttct
    31201 gaatcttttg atgaaatatc actaattgac tttgatgtac catttgctac catattacct
    31261 ttaacagtta atctatatcc atcatataaa tcttgtggtg aaactatatt taagtcacta
    31321 ttttttatag ttatttcagc taattggaca tcatcaatat ttttagctcc tgctatacct
    31381 agtttatcga ttttatttac tctaaatcct tctgtagaag gatctaagtt tgttccagtt
    31441 gaatcaactg gatttgctgt taaatcaatt ttttcatcac caatacctac atatatagtt
    31501 ctagaagtta ctgtttctgt tttatctctt aaagttataa ctaatttatc tttagcacta
    31561 tcatatgaag cttttacatc tgcaacttta taaattccat tagtttcagt tttagatttt
    31621 tctgttatag aattagcctc agcggaaagg tctgctgaat tttcatattt ttcagttgct
    31681 tctgcaacta cttcatttgc actatttgtt atatgccctt tatctgttat agttacaata
    31741 agttctttat tttctcccaa agcatttact aatttctcta attcatctat attagttatt
    31801 atttttaatt ttgataaagt ctcaccaact tttatttcat atattggttg tccaacttga
    31861 gaaccaccag tatacttttg agacattaaa tctctaacct tcgaaactaa ttctgtagct
    31921 tctttcttag ttattgtttc tttttttacc tgtgtagttt ctgcagcaaa aactggagct
    31981 actgatgtac ttatcattgc agcagccata attacagaaa gatttttttt attcatggta
    32041 ttttccccct aaaaaattca aatttttatt attttttcct tatttaccaa ttataatata
    32101 tatttgatat tatttcaata aatgagactt attgtaataa ttaaccaaaa gatacaaact
    32161 ttttcttata ttttattttt ttaatattta tgtatacaaa aaagtaacaa aattgtaatt
    32221 ttttttaata atccagttta taactaatag acttttcatc ttctttatag tataatatta
    32281 aatggaagaa agaaatttca agcatctctt ttttccgtat ttctcacaca gaaaaagaag
    32341 ctgattttca gcttcttttt catttgtatt ccttaattat ttaattgtaa ttacaatata
    32401 aagaatcaaa atactttatt tttcatttaa taaaaattac atgaactttt ttagttaaat
    32461 ttatataagt attgcactgt tgcttccctt atatttttat ttaaagcttc aaacatgtca
    32521 tatccttcta aagcatattc tttaaatggg tctttttgag cataagattt aagacctata
    32581 tactgtctta actgttccat agcatctata tgatctatcc agtattgatc aaccactttt
    32641 aaaagtactg ttttttctaa ctctgcaacc ttatcaatac caatcatcat ctttttcaag
    32701 tcataaactc tttttgaaat ttcatatgtg ctatcaatta tctcttggac acttttttta
    32761 tctacaccag gtattagtaa tgtatctgct ggcataaatg tactatataa atgtttaaaa
    32821 tatccataat aatctctttt tctcccaatt aaataagttt ctcctgcttc ttgtatgatg
    32881 tctttaacca ttttttgaat atcttcttgt atatcttcat catttaacac tttatttctt
    32941 tcattatata taacttttct ttgctcatta atagtatcat catatttaag aacatttttt
    33001 cttatttcaa aatttttacc ttctacaccc ttttgtgctc tttctatagc tcttgtaagt
    33061 gctttacttt caatggcagt attttctttt gagcttgttc tcttcataag tttctctata
    33121 gtttttccac cataaagctt tattacatca tcttcaagac ttacaaagaa tcttgatgta
    33181 cctgggtctc cttgacgacc agaacgccct ctaagctgat tatcaattct tcttgattca
    33241 tgtctttctg ttcctataac ataaagtcca cctaaatctt taacttcttg ttcttcttct
    33301 ttatctcccg cacctagaga aatatctgtt cctctacctg ccatatttgt agcaatcgtt
    33361 atagcatcta atttaccagc tttagaaatt atctctgcct ctttatcatg ttgtttagca
    33421 tttaagactt gatgcttaat accttgtttt ttaagcattc tagacagctt ttcagatttt
    33481 tcaacagaaa ctgttcctac aagtattggc tgtctagtct tatgtatcct tattatttct
    33541 tctacaacag cactatactt ttcttcttct gttttaaata ccttatcatg taaatcagct
    33601 ctaataactg gtttattagt tggtatttgg acaacattta atttatagat tgactcaaat
    33661 tccccttctt ctgtctttgc agtaccagtc ataccagaaa gtttttcata tagtctaaag
    33721 aaattttgat aagtcacagt agccatagtt ttagattcat tctttatctc aacaccttct
    33781 ttggcctcta tagcttggtg aagtccatct gtatatcttc ttccatccat tactcttcct
    33841 gtaaattcgt caactatcat tacttctcca tttgagataa catagtcaac atctttttcc
    33901 ataagcttat gacctcttaa agcttgattt atatgatgat ataattctat gttctttata
    33961 tcagtaaggt ttgttatacc aaaaaatgac tcagctttac ttatgccact tgctgttaat
    34021 gctatagttt tatcttttct gtccatctca aagtcttctt ctttaacagt ttttataaaa
    34081 ctatttgcta actcataaag ttttaaatct tcatcaccat ctcctgcaat aataagtgga
    34141 gttctagctt catctattaa tattgagtct acctcatcta ctatggcaaa gtttagttct
    34201 ctttgtactt tatgagataa atctggcacc atattatctc ttaaatagtc aaatccaaat
    34261 tcactatttg ttccataagt tatatcacat ttatattgtt cttttcttgt acttgggtct
    34321 tggttagata taattactcc tactgtcata tcaagagatt cataaactgg gcgcataagc
    34381 tccttatcac gttctgctag gtaatcattt actgtgatta catgtacacc cttacctgta
    34441 agggcattca aatatactgg tgctacttca actagagttt taccttcacc agttttcatc
    34501 tctgctatct ttccttgatg tattactatt cctccaatta attgcactct atattgacgc
    34561 attcctaatt ttctttttga tacttctctt actacagcaa aggcttctgg aagtatatca
    34621 tctaaagttt cacctttttc aagtctattt ttaaatatag ctgtcatatc ttttagttct
    34681 tcatctgata gatttttcat actatcttct agtgcatcta ttttatctac tataatattc
    34741 aatttcttta tttcttgttc atctgctttg tcaagtatcg aatctataac tgacatataa
    34801 gtacctcctt ttctcttgtc caattactat tatacattaa aaatccataa tagtagacaa
    34861 ttattttaaa ttatagtata atgattttac ttaaataatc aaacatcttt ttatttaagt
    34921 atttctcaca tattaattgg tacaaataaa aaaactctta acaattatta aaactaatgt
    34981 taagagtttt ttatttgata aatattataa ctgtaaaaaa gactcttcaa tgaagagcct
    35041 ttttattata aaaattttga ttacatattt aataaatctt taagcttagt tacaactgaa
    35101 gttgctatac ctttaccaac ttgaactaaa ttttcaccat cattatcacc aagtgcttta
    35161 cttattgcta cactttgatc agaagataat gaatctgtag ctaatactat tggagctgga
    35221 ctagtttcac caccagcagc atcaaattta gttccaaagt ttcctgctac agctgcagca
    35281 gctaaagcat caactaattg atcttcttta gtagaaccat ctttagctaa gaagaaattc
    35341 ttaactgtgc ctttttcata atagtcagag ttcttgataa cctcagcatt agtagcttgt
    35401 ctatcatcac cacttactct ctcaggagat tttccagtag cgtcatctat gtctctttca
    35461 acatctttag atacactatt ttctcctcct attatatcaa cttgagcatt tcctaagaaa
    35521 tctgaagcat ctgaacttaa agttttagct tttccatcaa caactactat aggagtagct
    35581 tcaccacttt ctaatttaga agcaactggt gctatactca tagcatctgc taaacctgtt
    35641 ccaccaacaa caaaagcttt atcattatct aaacctattt catcagcaat ttctaatgaa
    35701 gttgcatatc tatcatcacc agctaatcta gtaactttaa gtcccatatc ttttaattca
    35761 ttttcaacat ccttagatat agaatttaca ccaccagcta agtaaacttt tttactagag
    35821 tttacaccaa ctgtgttttt taagttcata actcttttta tttcagactt aacattacta
    35881 tctaatttat cttttgatgt taataataaa ggagctttct tttctgctgc taaaggagat
    35941 gctacaagac catcaactat agcatttgaa ccaactaaaa ctacactatt tacttctttt
    36001 tcaaataatg cattatcatc atctgaattg tagtattttt cacttaatgc tattgctgtt
    36061 tctattctat catcacctga aacatttgtt gaatttgagt atccattatt ataagttttt
    36121 aaatcatcta cataatcttt taaatctttt aatttgtcag ctttaattat taccttagct
    36181 gtagaatctg gatctgctat tgttgaattt acagctgata gagtatttaa tctctttcca
    36241 tctgcaaata tttcaacttg atacttacca ttaactcttt caatatttcc tgcctctatg
    36301 tcagtatata ttgaagttaa tgtacttggc ttaaatacat attttttagc taaagattca
    36361 gcagaatcat atgaacttgc atcaagatct attgttattt cttttgcatt tataattcta
    36421 acatttactg aatcactact tggatttgta tcagttaatg ataaccctga cacattacca
    36481 gttgatgctt caagtgcata agacgcagaa atatctacag ttttaccttt tactaaagcg
    36541 tctgtacctg ttgctgttat aactttagca tctggatatt taccagcacc tattgtacca
    36601 tatttcacat ctgatattgt ctttgcagtt atactaagct tatatccacc attagttgtg
    36661 tctgcttcaa cagtagtatt atataaatca gaacctttaa tatcattatc tgctgctacc
    36721 gcttcatcag cagaaccaga tttttctttt actgctcctg gattaactcc actagcctta
    36781 ggaattacta ctttattgtc taataatttt ttgtaatttt ctaattctgt ttttgtatat
    36841 tcttctatac ctttattata acttatttca aagtctacat acttaccatc gcctaaacta
    36901 tctaattttc cttctactgt attattaaaa tcatcagcta tcttatctct tgcatcctga
    36961 tcccctgcaa catttacagt tgaaacttta gttccatcaa aataaatgtt aatcgatgtt
    37021 ataatgtttt tctttatacc atcttgtatt tgttttactg ctttcttata gtctttttgt
    37081 gatacagtat acccttcact tccagttact acattagcaa atacaggagc agccgaagct
    37141 aacactgtta atcctgacat agctattgct atatttttct tattcatttc ttaaattcct
    37201 cccaacatta tattaaaatt acatctctta ttatgtacat agaataatca tccatatatt
    37261 cctaatattt ctcacatctc tataataatc catttttatt ctttttgcaa ggcatttttt
    37321 tgaaattatt atttttccca agcatatgag tttttatacc cttaaatttc aatatttaat
    37381 aacttaaaaa atttaatttt acttatttaa aaattcttga gtagcataca cttctccatt
    37441 aggcaatata tataatccaa acccagtata tacaaaatca ctatttaaca tattttcatt
    37501 atatttatct gactttttcc aaactgtaaa tattacattt gctagttctc tagcatcttt
    37561 tgttgtataa gtgctctttg taacaagtgg tacaaaagct atattttctt cacttctcaa
    37621 cccatatcca gtaaatactt caacagattt tttaccatct attacatgtg aaaattggcc
    37681 tttatctgcc atgtacttag accaggaatt agaaagccct tctaagtttt tagaaacttg
    37741 taatggctct ttaccttttt cttttctata tgaatttacc aaattataca tttcactaga
    37801 aaccattctt ttaaactcac tactattcat atcattagtc ttatattcaa gtacattcac
    37861 ttctacactt attgaggtag tctttccacc aatatcagta gcttttatag taactttgta
    37921 tttacccacc ttatcagtat taatatttcc tataagctct ggtgtcaaat cgttaccatt
    37981 gctatccttt gctactatat taagtttact tatatcaaac ttatcacctt gataaatatt
    38041 taaatttcca acagttattg ttggtgcact tgcatccagt gatgatgcac ttaaacattg
    38101 ttctagtgtc ttttcatcta ttcctcctag tgctgtgatt ttatcagcac ttctaagtac
    38161 agacttatca ctatttccat caaccaatac aataggtgca tttttagcta tagaagaacc
    38221 agcaactgca tctactaatt gataaccttt agaaagataa aattcttttg ctgaactata
    38281 aaaatgtttt ataacttttt tattagtctc aaatctatct tctccagcta atctaactgc
    38341 attagttttt ttaactaaat cattacttat tatctcctct gaaccaagag catagcattg
    38401 cacaccttct tttttctcaa atggaacatt tttaccatta gttaaaatta taggtgctcc
    38461 atctcttgat gctactgatg acgcactcat agcatctgct tcacctgtat aaccattagt
    38521 tataaaaacc ttatctatag gttttattga agctatttct tttgctataa gataacttgt
    38581 ttcaattcta tcattaccac ctattctttt aacttctatc cctttttgct ttaattcatt
    38641 ttcaactgat tttcctattg aatcttcagt tcctattata taaaccttct ttacatcttt
    38701 taacattttt tctgtgtctg atggtatact gtttctttgt gataataata taggtgcttt
    38761 aactgttcct gctaatccac ttgcacttaa tccatcagct agtgtattat ctgtatttac
    38821 taatactaca ttttcatatg tttgtttctg tgcaattttg gcagctgtct catatctatc
    38881 tttcccctgt atcttttcta tacttgaaag tgcattgata tttggtgctc ctgccattat
    38941 taatgatatt gtcaatccta ttgataatac tctcttattt accttcatca aactcctcct
    39001 actaattttt ttatataatt cagctaatca taaattacat cctcagtagt gattccctca
    39061 aaatctataa actactaaaa caaaaaacac ctaaaatacc atagcctgta aagcaataac
    39121 aaaacagatt acaaaaagta ctttagatgt gattttcatt ttataatatc agtaaattaa
    39181 caaaaaaaca agatttatgg aattttttac aagctacctt catttccatt taaaaactcc
    39241 tgagtagcat gcacttgacc atcagataat atataaagcc caaatccagt actataaaac
    39301 tcattactaa gcatgtttgc attgtactct ggagacttct tccacacctc aaatatagcc
    39361 tttgccaagt cttttgcatc ttgtgtcgtt tgaacgttct tagaatctat ataaatatat
    39421 gctatatttt cctcacttct cattccaaat tcagaaaata cttgtggagc attttttcca
    39481 tctatataat gtgcaaatac ttttttatcc atcatatact tagaccatgc atttgccata
    39541 ccttctagcc tactagacac taccaaaggc tctttgcctt tttcttttct ataggaattg
    39601 attaagttat acatttcagt agataccatt tttttaaatt ctgggctgtt gtaatcctga
    39661 gatttatcat ccaacacttt tatttctact ctttttcctg tactttttcc ccattcatct
    39721 gtagctttta atgtcaatac atatgtacct actctatttg tatctataaa cccatcaact
    39781 tctattggta ataccttccc tgtgttatct ttagctacta tatttaactt gccagtgtca
    39841 aacttttcac ccttatatac ctctgtactt ccaactgtaa ttgttggggg ttgtccattt
    39901 gactttaaag cacttataca ctgttgtatt accttctcat ttatttcacc tacagaggtt
    39961 atattcttag ctccttctaa tacagtttta ttgcttccat catttactag taccattgga
    40021 gagtttttag taagtggtgc agcggctatt gcatctacta attggtatcc atctgataca
    40081 tagaatccat ctgcactatt aaaaaagtag tctattacgt ttttgttagt ttcaaatcgg
    40141 tcagttcctt ctattcttac tgagtttgta tttttaacta gaggattact cattatctcc
    40201 tctgaaccta tacaatatga ttgaatattt tttacatcaa aaggcacact ctttccatct
    40261 gtaagtatga ttggagctcc atctctagta gctactgatg atacactcat tgcatctgct
    40321 tctccactat atgcattagt taatagtact ttatccactt tttttatagt tgctatttct
    40381 ttagctatta aatagcttgt ttttagtcta tcttctccac caattctttt gacttctata
    40441 tttttagaat ctagttcttt ttctactgat ttacttatag tatcttctgt acctattata
    40501 tatgcttttt tgatattttt taaacatcta tttgtgtctg ctggtatctt attttgttgt
    40561 gtaaatagta ttggagcttt tgtagctccc gataatccac ttgcacttaa tccatctgca
    40621 agtgattttt ctgtatttac taaaattact gtttcataag tctgtttatc agctatcttt
    40681 gctgctgttt catatctatc tttaccgtat attttttcta ctactgatga tgcatttaca
    40741 cttttgaagt ttactaagat taatgatact gctagaccta atgataatat ttttttgttc
    40801 attttcatat ccccctgatg atttttatat tttatattta tattgtgatt attttccatt
    40861 tattttctct ttattttcca tatattatag agtatatatg atatgaatat atctcgtcta
    40921 tatattagtg ttttatttta ttaaatttaa aaaattattt ttaacttctt tttaaacata
    40981 ttcaaaattg aatttgtatt tat
//
LOCUS       DACXMZ010000009        38599 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.009, whole genome shotgun sequence.
ACCESSION   DACXMZ010000009 DACXMZ010000000
VERSION     DACXMZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38599
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.009"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(129..641)
                     /locus_tag="JD016_01815"
     CDS             complement(129..641)
                     /locus_tag="JD016_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998366.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            967..1224
                     /locus_tag="JD016_01820"
     CDS             967..1224
                     /locus_tag="JD016_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998367.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            1367..1993
                     /locus_tag="JD016_01825"
     CDS             1367..1993
                     /locus_tag="JD016_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998368.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(2240..3019)
                     /locus_tag="JD016_01830"
     CDS             complement(2240..3019)
                     /locus_tag="JD016_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HAU4998369.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(3087..3512)
                     /locus_tag="JD016_01835"
     CDS             complement(3087..3512)
                     /locus_tag="JD016_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAU4998370.1"
                     /translation="MKDFEKLYEATTKLVSTSLKVKDDLKKMFKQNGYDITTDHYALL
                     RFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKDRRKFQIFLTDLG
                     RELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(3524..4201)
                     /locus_tag="JD016_01840"
     CDS             complement(3524..4201)
                     /locus_tag="JD016_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur flavoprotein"
                     /protein_id="HAU4998371.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            4434..4820
                     /locus_tag="JD016_01845"
     CDS             4434..4820
                     /locus_tag="JD016_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAU4998372.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVARESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            4823..6622
                     /gene="blaR1"
                     /locus_tag="JD016_01850"
     CDS             4823..6622
                     /gene="blaR1"
                     /locus_tag="JD016_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HAU4998373.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLVSWIIGILIMIAITIHCNIKIKNMKKSIQILRDDEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLKQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(6977..8851)
                     /locus_tag="JD016_01855"
     CDS             complement(6977..8851)
                     /locus_tag="JD016_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAU4998374.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(8856..10520)
                     /locus_tag="JD016_01860"
     CDS             complement(8856..10520)
                     /locus_tag="JD016_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAU4998375.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKNLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESEDVIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKAR"
     gene            complement(10534..11349)
                     /locus_tag="JD016_01865"
     CDS             complement(10534..11349)
                     /locus_tag="JD016_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAU4998376.1"
                     /translation="MYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICTGR
                     SMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNKRLIKEVVCLLKKREVAFSI
                     ESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKICLW
                     SNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKETIC
                     FGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(11454..12218)
                     /locus_tag="JD016_01870"
     CDS             complement(11454..12218)
                     /locus_tag="JD016_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAU4998377.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(12215..12964)
                     /locus_tag="JD016_01875"
     CDS             complement(12215..12964)
                     /locus_tag="JD016_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAU4998378.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(13279..13461)
                     /locus_tag="JD016_01880"
                     /pseudo
     CDS             complement(13279..13461)
                     /locus_tag="JD016_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426285.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13468..14073)
                     /locus_tag="JD016_01885"
     CDS             complement(13468..14073)
                     /locus_tag="JD016_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAU4998379.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            14252..14890
                     /locus_tag="JD016_01890"
     CDS             14252..14890
                     /locus_tag="JD016_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAU4998380.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            15022..15684
                     /locus_tag="JD016_01895"
     CDS             15022..15684
                     /locus_tag="JD016_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4998381.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLRKKLKNPPCIKTLRGIGYRME
                     DN"
     gene            15684..16865
                     /locus_tag="JD016_01900"
     CDS             15684..16865
                     /locus_tag="JD016_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU4998382.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLTVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTIHAENCHNGGARFIIKFY
                     T"
     gene            complement(17046..19706)
                     /locus_tag="JD016_01905"
     CDS             complement(17046..19706)
                     /locus_tag="JD016_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4998383.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(19703..20386)
                     /locus_tag="JD016_01910"
     CDS             complement(19703..20386)
                     /locus_tag="JD016_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4998384.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(20730..21683)
                     /locus_tag="JD016_01915"
     CDS             complement(20730..21683)
                     /locus_tag="JD016_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HAU4998385.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLINKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGYTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(22224..24338)
                     /locus_tag="JD016_01920"
     CDS             complement(22224..24338)
                     /locus_tag="JD016_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAU4998386.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSRFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            complement(24422..25258)
                     /locus_tag="JD016_01925"
     CDS             complement(24422..25258)
                     /locus_tag="JD016_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4998387.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            complement(25853..26215)
                     /locus_tag="JD016_01930"
     CDS             complement(25853..26215)
                     /locus_tag="JD016_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAU4998388.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            complement(26513..26977)
                     /locus_tag="JD016_01935"
     CDS             complement(26513..26977)
                     /locus_tag="JD016_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAU4998389.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            complement(27406..28827)
                     /gene="nhaC"
                     /locus_tag="JD016_01940"
     CDS             complement(27406..28827)
                     /gene="nhaC"
                     /locus_tag="JD016_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAU4998390.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            complement(28857..29921)
                     /locus_tag="JD016_01945"
     CDS             complement(28857..29921)
                     /locus_tag="JD016_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HAU4998391.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFRINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            complement(29906..31315)
                     /locus_tag="JD016_01950"
     CDS             complement(29906..31315)
                     /locus_tag="JD016_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HAU4998392.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            complement(31315..33519)
                     /locus_tag="JD016_01955"
     CDS             complement(31315..33519)
                     /locus_tag="JD016_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HAU4998393.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            complement(33513..33887)
                     /locus_tag="JD016_01960"
     CDS             complement(33513..33887)
                     /locus_tag="JD016_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase"
                     /protein_id="HAU4998394.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            complement(33965..35377)
                     /locus_tag="JD016_01965"
     CDS             complement(33965..35377)
                     /locus_tag="JD016_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HAU4998395.1"
                     /translation="MKDMSYQAVMGKNNEIMKNAIGLDYSSFEQEGIGFDYEKMMSET
                     GYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNASGSFKA
                     RRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNGVGQPEI
                     IEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVETLGYELA
                     IQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLHMASDNQ
                     FNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTTELLAQL
                     EGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARENGIDIK
                     FGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKLSNRDIE
                     FLMEEVKSSRDFVVDVLNNL"
     gene            complement(35388..35696)
                     /locus_tag="JD016_01970"
     CDS             complement(35388..35696)
                     /locus_tag="JD016_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HAU4998396.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTIIGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            complement(35794..36861)
                     /locus_tag="JD016_01975"
     CDS             complement(35794..36861)
                     /locus_tag="JD016_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HAU4998397.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            complement(37117..38517)
                     /locus_tag="JD016_01980"
     CDS             complement(37117..38517)
                     /locus_tag="JD016_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAU4998398.1"
                     /translation="MNQEIFFKIMNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMN
                     RHDILANSFFDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPID
                     IKGEFVGALEISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAI
                     EKSKKACKSDASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGIL
                     FGTSKGGFTGAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTK
                     DIPIDVRIIATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFI
                     SKYNKKLNKKVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINL
                     NDYYNLKENKESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMN
                     RQNLQYKIKKYNIKIL"
BASE COUNT        12830 a         6612 c         4334 g        14823 t
ORIGIN      
        1 aaaaatagag ttaccaattt tattttggca actctattta agtttatgtt ttacagttat
       61 attagattta aacttagttt cattatcaca ctaacttcaa atttactcta taatctttaa
      121 atcaaacact aatctattaa tttatatttc tttagacatc taatatagtt tgctactaaa
      181 taataattat ttatatcata tttatctaat cctttaaaag aagttatttc atcattaagc
      241 tcatcactac cttctacaaa aacacaacct gtttcaaatt ccatacctac aataatctta
      301 cagccactat aaatttcctc ttgagtatcg tacaattttt ttgcatttcc ttcatttgga
      361 attgtaaata caataggttt taattcttta tcactggaaa tcttttgtga aaaaggcttg
      421 tttaacttaa tattattgat aactacatgt ttaaattcct ctgaagtttc ttctactatt
      481 acatattttc gctttaaaaa ctctattatt tctaaaacag acttagaatt tacttttaaa
      541 atacttttat atttttcata taaagatttc cactgttcaa ttttttcctg tggtatttct
      601 tggatatgtg tacttatttt ttcttgagat atttttttca attaaactct cctttcaata
      661 cttaaatatc cttatgaatc tcacatgact aaagttacaa ggtttctact taatagaatt
      721 ttgcatactt aaatatgtct tattaattct ctacaggata tctctgtaat tcctacgatt
      781 cttacataag ttacttattt taaattgtta ttagttagat tctaaaaatt atttatttta
      841 tcactaatat aatactctta aaaactttac aaatcaattt aaatttgcta ccttttattt
      901 ataacaactt atagtacatt tctcctatgc ttttcaatat attactttta aaaggaggtt
      961 ttcattatga ttttaaatag agctgagctt ttccgaggat attctttaaa tagatttaac
     1021 gagatatgca atatattatc agtaaataaa attaaatata agtataaagt gaaaaataat
     1081 gcttactcta aaaaaccttt ttttaatgaa ataacacttg gcaatttagg tcaaaaggaa
     1141 gatttctctt atgagtactt aatttatgtt cataaagacg attatgaaca ggcagagtct
     1201 ctaataaatt ctaagttgcg atagattatt tataaaacat gacattctgt tgtcatgttt
     1261 tatatgttat tatactttca aatatgctac tatattttca aaattgtgtg ctttaaaact
     1321 agctttttat agtaatttaa agcttaaaac taaggagact cgttatatga aaataaatat
     1381 aaaatcaaag ttaagtgaat ttattaaaca gaacaatctt tttgatgaca gcagaataat
     1441 atcctacctt cctaatataa caatagaaac agacaaaata aaaactataa tgattaatga
     1501 ggttgtccca actaacacca atgacgattt ttatagtgta gataaagatg ctgattattt
     1561 aaaaactact attcctttat ttgacagtgc aggaataaaa gtatctaaca taaatgatat
     1621 cctagatatg ggtatctata taactaatgc agtaaaactt cctaaatcag aatatacaat
     1681 aactcgtgat acaattaaat tacatatgcc tattttagaa gaagaaatta aattatttaa
     1741 aaatctagag gtagtaatgt taatgggaga tgttgcaaaa aaatctttta atatgattac
     1801 taaaaagcat ataaaaaaga atgtcatccc ttccatttca acatataaac ttcgtaataa
     1861 tgaactttat tatgataatt taagagtatt tccatcatat attatgactg gtggaaacat
     1921 actgattgaa aaatcaaaat ttgaaatggc atcagaagat ataaaaaaaa tgttcgagat
     1981 tataaacggt tgatattaca aaataataaa aactaatata aaataaaaag tgcctcaaaa
     2041 acttctattt atgaggcact tttattttat attagtgtaa ctatatacta ttaacataat
     2101 ttattattta tattgttctt tttaatctct attccattta gtctaggtaa cattactctt
     2161 tacaagatta ctttatattt cttttatatc tctaacgttt ttatttaata aaacaattat
     2221 taatacaagt caaatatatc tatttatcta aaaatatatc ttttacagta atatctggat
     2281 ggtgatactt cttcctattc gttaaaaaag atttttttac attaatagcc tcttttggac
     2341 accagtgaaa acaagctagg catctttcac actcattacc ccactttggc tttttatcaa
     2401 ctatacttat attctctaca ggacaaaccc ttttacatgt cccacatcca ttacatttat
     2461 tttctgcaaa aaatttagta gttgcaattt gcatattctt acctactaaa cgaagtacaa
     2521 cactatctat atgtgacttt aaattgtttt ttccttctat atttccaatc tccaagttat
     2581 tgatacattt tgcaatctca gctaccctat tagtctgatt tttcaatctt tcttcttcaa
     2641 catctggagg tgttatcaaa gcatttccag gcatattgat taaaaaacca gcagataatt
     2701 ttttccccct ttttttaagt aacttatcta tagtaaataa actatgtcca gctaccccat
     2761 tacaagtggc aatggcatat atatgagtat taatattaaa atttaatttc tctacaaaag
     2821 actttactat atcaggaaca tcagcaaaat aaacaggaaa aacaattacc aatatatctt
     2881 catttatgtc tatattattt tcttttctat gtatagctaa gggtataact tctttacttc
     2941 catttaacgt ttcttttaaa atatttgaaa ctactagcga atttcctgtt ccagaaaaat
     3001 aataaatctt actattcatt cttatccctc tttctatatt tctacatcat caatagttaa
     3061 aaagtctctt aaaataggat ttatatttac tttccaattc tatccaaaat atcagtaaac
     3121 aaaggcaatt cttcatcaga gatagattta aatgtctctg ttgcatatat acttgcaatt
     3181 tcatttaatg gttcttctaa ttctctacct aaatctgtta ggaatatttg aaacttacgt
     3241 ctatctttaa catctacttt acggacaatt aatcccttat ttttcataac atctaaaatt
     3301 cttgttgtat tagatttatc cttacatgat ttttcacata aatctatctg agaaattcca
     3361 tcttgctccc atagaaacct taataacgca taatggtcag ttgttatatc atatccattt
     3421 tgtttaaaca tcttttttaa atcatccttt acttttaacg aagtacttac taacttagtt
     3481 gtagcttcat atagtttctc aaaatctttc attttacctc catttaaata atcccttgct
     3541 cctcccatct atcaattatt cccttatatt gctcaatatc tttaaaagcc attggcttta
     3601 aaacagcatt tatagcaata aaattataga tttgagaaag aacaggtgga tttttctttt
     3661 cctctatgca ataaataaat tttttagata ccttttgtat ctttgattct actttttcag
     3721 ttataggatt ttgattaaat ccagttttta tagataaact accacaaaca taacctcccg
     3781 aatctaagac catggatttt ataaaagaaa ttgcttttga accacgtgca ttttcatatg
     3841 tagtaactgc tatacatggc ttacgataca ataatctttc aagcatcata tgtgctctat
     3901 ctgtaaacac cttgaaaagt ccagttacac tacttccata agttgggcta ccaaatataa
     3961 caccatcact cttagtaatt atatcatgaa tatactctac tttatcattt tgattgatac
     4021 acttgcccat tttataacaa tttaaacaac caatacaata tgatggattt acctcactta
     4081 aatcaaaata attaattttt acatctggta ttaatctttc tatatcttta tacaagtaag
     4141 ttaaaacttt tgatgtagct ccattttttc tagggcttcc atttataact gtaattatca
     4201 tttctatatc tcctttttag ttgttatatc aacaataagt atatcaacta tatttttagc
     4261 tgtcaatata aaccatttat ttaaagccat acttacacag cttaaataaa agttttattt
     4321 aactacatgt gcttatttaa tcaactataa atgaattatt tttaataatt gacaaactaa
     4381 attaatgata atatacttta tgtattacaa atgtaagtag aaggaggtat agtatgaaaa
     4441 agttaccaca tatttcagaa gcagaatatc aagttatgaa aattatttgg aaatatgctc
     4501 ctatcagcac tactgaagta attgaaaagt tggtagaaac aagtacatgg agtcctaaaa
     4561 caattcaaac aatgttattg agattagtta aaaaaggagc tttaacatat gaaaaaaata
     4621 gcagagtatt cgtttatact ccacttgtta aagaagaaga atacgtagct agagaaagta
     4681 gttctttttt aaatcgtttt tataatggta ctcttaattc tatggtacta aactttttag
     4741 aaaatgataa actatctgaa gatgatattg aggaactgag agaaatctta aataaaagga
     4801 cgactaaggg ggataaataa atatgtatgc cccattcttt atacgatttt tactcagtac
     4861 attaacctta tcaatattaa ttgttgtaat tttacttgcc aaaaaagtat ttaaaaaaca
     4921 tatttctgca aagtaccaat acaatattgg atttatattg cttataatgt taataattcc
     4981 atttgtccct cttaaatata ttaatcttgg aaatgtattt aactatttat atacttttaa
     5041 tcaacttgga agcagtacta ataggacttc tatgataaaa aatagtccaa attctataat
     5101 taataactca aatgcattaa acgatttttc agtatcccta aaccaagtta attttgatag
     5161 tttaaatatt ttattactag tatcatggat aattggaatt ttaatcatga tagctattac
     5221 tattcattgc aacattaaaa taaaaaatat gaaaaaatca atacaaattt taagagatga
     5281 tgagatagta tttatatttg aaaagtgtaa aaaagatatt ggtataaata aggattttat
     5341 actatgcaaa tcacctatta taaatacacc tattacattt ggatacttta aaccacacat
     5401 tatattacca gataagtcta tatctaaatt atctttaaaa gatataaaat atatcttact
     5461 tcatgaatta caacatttta aaaataaaga tatactgata aattatataa tgtgctttct
     5521 tcaaatatta tattggttta atcctcttgt atggtatgca tttaaagaaa tgcgtataga
     5581 ccgtgaaatt gcatgtgata tttcagtttt aaaaaagctt gataaagata gtcatataga
     5641 atatggacaa actctaataa actttgtgga taatatttca agaccatcaa atcttacact
     5701 tacatcagat attggtgggt caaaaaaaca aataaaaaag cgactattga agataatgga
     5761 ttttcataat gaatcaaata cgttaaaatt caaaagcatt ttaattttta cattgatagc
     5821 tttaattgta tttggaaata ttcccaaact atctattatg gcagatatca acgaaaaata
     5881 tagacctaca aatgaacaaa ctgtgtacga ggacttaagt cagtatttta aaggttttga
     5941 tggaagtttt gttatgtatg atttaaaatc caagcaatat catatttaca ataaagaaaa
     6001 aagtaaaaca agaatttctc caaattctac ttataagatt tatagtgcct tattgggatt
     6061 ggaaaatggg ataataacaa gaaataattc acatatccct tgggatggaa agcatcattc
     6121 taatagttct tggaatgaag accaagactt attttctgct atggaaaact ccgtaaactg
     6181 gtactttcag actctagata aaaaaacagg gctaaaaaac atacaaactt atcttaagca
     6241 aattggatat ggcaattatg atgtttcagg tggtatctca aacttttggc tagaatcttc
     6301 attaaaaata tcacctattg aacaggtaga attacttgaa agtttttata caaataagtt
     6361 taaatttgaa gataaaaata tagaaacagt aaaagattca ttgttaatat caaaaaagga
     6421 tggggtatca ctttttggta aaacaggcac tgctaatata aatggaaaag atgtaaatgg
     6481 ttggtttata ggttatgtgg aaaaaaatga aaatacttat ttctttgcta caaatattca
     6541 aagcaaagat tattctaatg gtagttcagc atctaagata acattatcca tattaaatga
     6601 caaaaaaatc tataatcctt aatatattta ctgtacattc taaaaaattc acataatttt
     6661 catattaatt atcgattaaa ttcatataca aggcttatta tataaacata gaagatagta
     6721 aaactagctt aaaaacataa tataataccc tatacaaata gtggcaactc tccctcacat
     6781 cctcaattgt tgccactatt taattttttg tatgaagtta aatatatttt catcatttca
     6841 acaatttggg tgcaatacaa tctctatttt gaaacaacct ctttaattgt tttagagttt
     6901 atgaattatt tctcatactt taaaaagatt gaattactct tgataatctt cttttgtagt
     6961 cattacatat cacctactac tcaacacata ttctttgacc tatttttaca tccttgttta
     7021 tatccaaaat atttaccttc ttaccatctg gaaacaatac cattgtcaaa gtagactttc
     7081 ctaattcgtg tattctcttt aaatcaacat taacaagaac atctccctgt ttaaccatat
     7141 ctccttgttt aacagcaaca tcaaagccat ctccatttaa tgcaactgta tcaattccaa
     7201 catgaattaa tacttctcca ttagttcctt taatcccaaa tgcatgtcca cttgaaaata
     7261 ctgtttctaa tttacctgaa ataggagaaa caatttttcc atctataatg tcaatagcaa
     7321 aaccatcacc taacattttt ttagaaaatg tttcatctgg tacattttct aatgcaacaa
     7381 ttttcccctt agctggagac acaaagtttt cattattttc attaaacact aattcttcgt
     7441 cttcatcttc atttatagct tctttaggca ctccaaataa acatgttaca ataaagcaga
     7501 atgcaaccac aaatattgat actaataagt atgtgattaa attatatcca tcatagatat
     7561 acattaaata agaaggtaaa actgctaatc catatgaatt agattgaata tgtaaaattg
     7621 ataatattgc tgctcctaaa cctgaagatg cacacataat aatcaaaggc tttaaattat
     7681 atcgaagctg aatcccaaat aaaacaggct cacttatacc aaaaagttga gatagcattg
     7741 ctccaactgc agtttgttta acttgattat ttttagattt tttcataaat gctagacatg
     7801 ttccaatatt cgcaaatcca tacattgcac aaagagtaat aagggcatta aatcctgtat
     7861 ttgctaatag tgatgtttca atcattacat aagtatgatg taagccagta ataacagcta
     7921 atggatatgt aaatccaata ataaatcctc caattcctaa aggcaaatca atcaataagc
     7981 tgatgatatc aaccattttt aattctattg tatgcattat agggcctaca cctaacaaaa
     8041 taactaacat tgttattagc ataacaaaaa atggtgtgaa aataaggtct aatacattag
     8101 gcatatgttt acggaaccat ttttcaagat tagctccaat aaatgctgta acaatagctg
     8161 ttaaaacact tccttgacaa cctacaatag gaataaatcc aaatgccata attgcttcaa
     8221 tttcatttga tgggtcagca acagaatatg cattaggaag tattggggaa actaacataa
     8281 gtccaataac aagtccaata acaggtgttc ctccaaatac tttaaatgct gaccatacaa
     8341 taattgctgg taaaaaagca aatgctgttt ctgtaagaac cgaaacaagt gtctgaatat
     8401 acaatggaat atttgcagaa gacattccaa ataatccaag tacattatca ttaaacaaac
     8461 aaccctttaa tcccaagaat aatcctgttg ctgcaataac tggaataata ggaacaaata
     8521 tatctgctag agttctcata actttcttaa atccagtttc attattttta actaattcat
     8581 cttgttcttt ttttgataaa gtttttaatc ccattttttc aacttctgca taaactttat
     8641 taactattcc tgttcctaaa ataatttgat actgtccaga agtaaagaaa acccctttaa
     8701 cctcatcaac tttttcaact ttcttatcat taataatttc tctatcttta accatcactc
     8761 ttaatcttgt ggcacaatgt gtaatagaaa taatattatc ttcaccaata attttaataa
     8821 gctcatttgc tattttatta tatttattca tttttctacc tcgctttcaa taatactgca
     8881 tgaaatggtt tcaaacgcac tgttttattt aatagattta tatcatctaa attttggaat
     8941 acaatatcat atttttcttc tactcttatt tctacaggtt tatcagaaaa attataataa
     9001 caatcaataa ctaccaaatc taattgcctt ctatacgcaa taacatcttc actttctata
     9061 tcaataggtt ctattgaacc ataaatcaaa caatcagaat actcactatc ttgtctcaac
     9121 tttatcatct ttttataaaa ttctaaaata ctctctgaat tgttaatctg actctgtgca
     9181 ttaatatctt tataattttc aataaccttt aaccatggtg ttgtatctga aaaacctgca
     9241 ttttctttat ctgcccatgg aaatggcgtt cttgcattat ctcggcttct taaattcaca
     9301 aattctaacg cttcttcttc tgagtaacct tcatctattg aacgatagta ttggtcaatg
     9361 ctagataaat cattaaaatc atttatggta tctcttttaa aatttgtcat tcctaattcc
     9421 tgtccctgat aaataaatgg ggttcctctt aaaaagaaat acattgctcc caacattttt
     9481 actgcatcat cattcctttc gtattgcaaa agatactttg ttgtagctct tggttggtca
     9541 tgattttcaa taaaattagc tgcccatcca tgttcctgaa catacatttg actatctaaa
     9601 atgcattttc ttaaatctgg aattgtccat gaaattcttt taaaccactc actaccagaa
     9661 gcaacatcta agtcagcatg tttaaagtca aagaccattg aaaagaaacc atcttctcca
     9721 ataaaatcac ctaactggtc atagctaata cctgctgctt cagcaacagt catacaatca
     9781 tattttttaa acgtattttc ttttaattca taaagcattt tttctattcc aggctgattt
     9841 ctaactgttt ttgtacattt aacaagacca tcaacaccat cactttctaa actttgaaaa
     9901 cttaaattct ttttgataaa tgtaatagca tcaactctaa atcctgccac tcccatttga
     9961 gaccaggtat taatcatttg gtaaagttca cttcttaata ttggattttc ccaattaagg
    10021 tcaggttgtt ttttaccaaa agaatgaaaa taatattcgt ctctattctc taccttttcc
    10081 caaacacttc caccaaaaac agacctccag ttatttggta aatcgtttcc ttttttaaaa
    10141 atataataat catgataagg actttctgga ttttttaatg cttcttgaaa ccaaggatgt
    10201 tcatcacttg tatgattaag aactaaatct aatataattt tgattcctct atctttactt
    10261 tctttaatta actcttttaa atcttccata cttccaaatt cacttgcaat atccaaataa
    10321 tcactaatat catacccatt atcgtccata ggtgatttaa aaattggaca taaccacaat
    10381 gatgtaatac ctaaattttt taaataatct aaacgagaaa taattccctg taaatctcca
    10441 attccatcat tattactatc ttgaaagctt cttggataaa tttgataaac gatttctttt
    10501 tgccaccatt tcttttccat aaattactcc tttttatatg atatttcttc tttttaattc
    10561 tttataaatt ccattatcga atatgtcctc acatatagat gtcgctatat ctttaagttg
    10621 ttgatgacta tttttcattg caattgtaac gtctgatgct tgaaacatga caatgtcatt
    10681 ttgaccatca ccaaaacata tggtttcttt ttgagttact cctaatcttt cttgtaatct
    10741 cttaattgct ttgcccttat gaaaatcttt ttgaataatt tcataatact gactagaaat
    10801 atctctctgt gctaattcca ttttatcttg tagtatatct ttcacttcgt caaatacctt
    10861 ttcatttgac cataaacaaa ttttatgtat atcttgtgac ttatactctt caatgttatt
    10921 ttcatatgtt attttttctt ggatatgttg tttatttata caagaattag ttcctttcaa
    10981 ctgattcatt gtttcaaata tttctttggc tttttgattc atgaaaactt tttcctgact
    11041 ctcaatcgaa aaagcaactt ctctcttttt taataaacaa acaacttcct ttatcaatct
    11101 tttattaaaa gattgattat aaagtaattc accatgatat tgaatgtaat tacctcctcc
    11161 tgcaatatat ccatcaactc ctaatgataa aacatcatct tgtattgtac ccatacttct
    11221 tccagtacaa ataacaacac tgcaatgatt cttttgacat aaacgtatag catgctttgc
    11281 agattctggg attccatata cctcatctct aagcgtacca tctatatcaa gtattaataa
    11341 tttgtacaat ttcatcttcc tttcttacta tcaaaatcat aatagcattc aaaaaaaaag
    11401 atatcaatat tttgatatct tttaaaaaat atgttcattt ttagcacaaa ttttcatttt
    11461 cggggaatat gttcactttc taaatacatc ttgtatttaa tataaagcag ttcaattaaa
    11521 ataaaataag gtgttgttac atcgtagtcc agttgtttat ctacagaaat agatatagat
    11581 tgaatataca ggttttcatc acataatctt gctaaaggat tacttatcat ttttgtaata
    11641 gaaaccatat aaattccttt cattctcatt tctttagcaa aatcaataat tgtagatgca
    11701 tctccattca atgaaataaa gaaaatcata tcatctgttt ttataaaatg attaagtgcc
    11761 tgaatcatat cataaccata aacatcatat ataattttcc ctgttggtaa gaaaatcctt
    11821 ttcatctctt tagcaactct ttcctgagaa taaccttctc caaagacgat tatacgattt
    11881 gcctttttca ttctttcaaa cataatcgaa caatccttct tctctatatc atctatcacc
    11941 ttattataac aatccattaa ttggtgttta ctttgatttt tatattctac ttctgtttca
    12001 tttaagcgta taataactct taattcacta tatccaggta attgtagttt ttgagcaaaa
    12061 cgagaaagtg cacttgtaga tatatggtat ttgtctgcaa attcatctat agaaagttta
    12121 atacaatctt ctttatgatt aagaatacac tcacagatat acttatcatt tgctgtaaat
    12181 gaattataat atttattgaa aagtccttca attttcattc ttttctcctt ctatactcta
    12241 tgtaattcat atgaagcaaa tctaatagag cataaaatgc tcctacgact tcataagata
    12301 tagcttgtaa tgtatcaagt ttttgcgttt caacatacag attatactca caaagtgttg
    12361 caattgtatt attatcaagc ctagttaaag aaatcgtatg tatatgattg ttgataaatt
    12421 ttgcaatttc aattcctgct tttgtttctc cagacaaaga aatgataata aataaatcac
    12481 ttttttgaaa tgattctctc ataaattcaa tttcacctat atcaaataag tcaataacac
    12541 atttacctac tgaaagaaat attctcttta tttcttgagc taaacttttc tgttcattac
    12601 ctgtcccata aatataaatt gtttctgcat catatattat tttacaaata tcttgaaatg
    12661 atattttttg taattcattc attaatagtc gataattctc gcaaacatca tgaaaattat
    12721 tatcattatt aacaaattct agttgattat ttttaagtaa atactttaaa tcagagaatg
    12781 aatctaatcc aatttttcta caaagcctta ataaagtagc tctagaaaca ttacattcat
    12841 ttgcaatttt ttcacacgaa aaattacggt attctctttg acaaatcaat atttgtttaa
    12901 taataatttt atcattttca tttaaattat cataattttg gttaactaac gattctaatc
    12961 tcattttttc cactccctaa atattatttt aatatacatt agttgctttt tctagggaag
    13021 agttttaaat aataaggata aaataatcat ttgattttat ttttattaaa ataaaaataa
    13081 cctgctaaaa gcaggcacca aaaagtactt atcctttcgg acatgtactt tagatattgc
    13141 tttcttcaga agcttcatct cttcatactc taaaatagag ttttttctaa ctcaaaagta
    13201 ttttccatgg tagaaattac tttaattcat ctgccattta ttttttatct tatttaataa
    13261 ttactattac ttgaatattt acttctttaa gtaaatttca tagttaacaa agctaaagct
    13321 attattatag atgttaaaat acaaaattgt aagtattgtt ataaatatgc ttacagctac
    13381 aaatatacat gatatattaa aaagaagctg taaattatta tcatacatac aatcaactac
    13441 gcctctatta atccatgtta atttttatta tttaatacaa ggagttccat gagacataca
    13501 tatccattca tattttaaat tatccaattc tcttatagat ttcatcatct ttttataatc
    13561 ccaattccat ggattaggat aaggaactat atttccattc ttatttttga ctaaatcacc
    13621 tgcaaataat acatcttcaa aaatcatgca gacatgccct ggagtatgac caggagtatg
    13681 gataattttt atattcccaa tctgcatatt ctctccataa ggttcaacat ccttaatgag
    13741 ccttctggaa attgcctttc caataaactt cttaaatcca ggtctatcct tctctcctat
    13801 aataaaaggt atatcctcta catgagccca tacttttgct cctgtttttt ctttcaataa
    13861 tttaatatta gatatatggt caacatcatg atgagttaat aaaatatgct taatatctgt
    13921 aagtttaatg ccaagtgttt ttaattcttt aattatattt ttccccataa aaggaagacc
    13981 tgtgtctata agaataattt ctttatcaaa cacaataaaa caatttgagc cacgagtact
    14041 atctaacatt aatacatttt cttttatctt cataaataaa ttccccttat ttggtatttt
    14101 acctatataa ttaattaata aagttataaa tagttataat acttgatatt aattttattt
    14161 tttattaaag tatactatag gtatacaagt attatattta cataagtata ccatcagtat
    14221 acttttttat caatagaaag gaataattta tatgtcagaa ttatcaaaaa gagataaaga
    14281 aaaaatacaa agagaaaatg agattattga taaggcagaa aaattatttt gtcttaatgg
    14341 atttgataat acaactatga atgaattagc aaaagaagtt gaatatacta aacgcaccat
    14401 atacaagtac ttttcatgta aagaagattt attctttgct gttgtcttaa gaggatataa
    14461 aagactttgg gataatgtta aaatagaaag tgcaaaaggc aaaacagggt ttgaaaaaat
    14521 aaaactttca tactttgctt ttcataaatt ttactctgta gaaccttctc tattatgcct
    14581 tatgggaatg attggtattg taaactctga aaaatcggat actgatatgc tgtttaaaga
    14641 aaagtttttt tcttttaata aatttatgtt tgatgaaata caaggaatgt ttgaaattgg
    14701 aaaaaatgat agaagtatta ggcatgatat tgaaatacct actcttatgt attcatcaat
    14761 atttacactt acaggatttt ttaatttact atctgtaaca ggaaaatcat acctgaacaa
    14821 ctttaatata gatgaggaaa aatttattga aacaactctt gctcttttaa tagattcaat
    14881 taaagcataa acctaagttt ataaattaaa gtttatatac ttaaatgata attttttcta
    14941 tctctaatat aaaaaatatt aaacataaaa ctaataactt agttaatgga ataactaaat
    15001 tactgaaagg attaaaagta aatggctaaa atacttcttt tagaagatga tgatacaatt
    15061 acatttggaa taaaaacttc ccttgaaaag aaaaatcaca ctgtatgttg ttataatacg
    15121 attgctgaag ctaaaaaaaa ttttgataaa actattgatt taattcttct tgatttaaat
    15181 ctacctgatg gtattggata cgagttttgc caatatgtta aaagtataga agatactcca
    15241 gtaatatttt tgacagtccg tgatgatgaa aaagacatta tacgtggatt agatatgggt
    15301 gcagatgact atgtggttaa accgtttttg ctctcagtac ttcaatccag aattaatgca
    15361 gttcttagaa gaactcattc aaatactaac aatactatta catgtgaaaa tataagcttg
    15421 aataaggttg aagtcaaagc atatttagat aatgttgaag tacctttgac agcaggagaa
    15481 tataaacttc ttctaagtct tatggaaaat aaaaatagag ctatgactcg tacaaagtta
    15541 ctagaaatgc tctgggatgt agatggtaat ttcgtaaatg ataacactct tactgttaca
    15601 ataaaacgtc ttagaaaaaa attaaaaaat ccaccttgta taaaaacatt aagaggcata
    15661 ggatatagaa tggaggacaa ctagtgaaaa ctccaagaaa tatcaacagt acagttgttt
    15721 ttataatatt ctgcttaatt gtaacttttt tattgactgt aattctaaca actataactt
    15781 cttttaatga atacaatgta ctgtctaagg ctcttggtgc atcatatgag tacaatccta
    15841 agtctacaga aagtatacta aaatcactga aaaattctag tgataaggat tttaaaacag
    15901 gaaatgaaat tttatcaaaa catggatatt ctaaaagtac attttggagt aaaaactttt
    15961 tttattttct agtgatatcg tttggtataa tttcaacaat ggctacagtt atatatataa
    16021 tcaaacatag aacaaataag caaaaactga gtagaataaa caatcttact caatacttag
    16081 aagaagtgaa tttaggaaaa gagtctgtac ttttacgatg tgaagatgaa ttttctcacc
    16141 ttgaagatga gatatataaa acagttgggg aacttcgttg ttcaaaagaa tttgctttaa
    16201 aagaaagaca aactctttca gataatcttg cagatattgc tcatcaactt aagactccaa
    16261 ttacctctat gtcacttatg gcacaattac tttcagaaaa ttgtccagat gaagaaatag
    16321 attatattaa cagacttaca aatcaaatct caagacttga gagactagtt tcatctttac
    16381 ttacattatc aaaattggat gcaagtactt taatatttga aagcaaactt atagatgtac
    16441 attctcttct tacttatgca attgagccta tagaaggttc tttaagagaa aaacatcaga
    16501 catttactat cgaggcttct ccttcggtag aatttgaaat cgatattaat tggactttag
    16561 aagctctttt aaatattctt aaaaattgta gtgagcatat agaagatggt ggatatatag
    16621 ttgcttatta tagtcaaaat cctctatatg ttgagattat catagaagat aatggtaagg
    16681 ggtttgctca ggaagaactt ccatatattt ttaatagatt ttatcgtggt aaaaatgcta
    16741 gtaaggacag tattggtata gggcttgctc tatcaaagtc tattatagaa cgtcaaaatg
    16801 gtactataca tgcagaaaat tgccataatg gtggtgcaag attcataatt aaattttaca
    16861 cataaaatgc tggattatag gtaaaatacc tataatccag ctatatctat attctatgtt
    16921 atctattgtt ttagtaatat acttctattt acatttaagg ttttattaaa caaacaactt
    16981 aactatccct atacttgtaa gtagtcttaa atcgaaacct ttaaataagt tcaaatagat
    17041 tttgtttata ttgtttcatt ttttatagat tctactatat tttcattctt caactgcttg
    17101 tatcctgtca tatatgatgc tattacaata aaaagtatca atatagtaaa acctattatt
    17161 gtagatatag gtgcaaatgg taaatactct ataaaatata tttcatttat acgtagaaaa
    17221 gcatacacta ttcctatttg cacaggaata ctaagtagta atggtgtaat tcctaagaat
    17281 aaaccttcca aaataagcat cttttttatg ccttctggag ataatccaac agaacgcaac
    17341 atagcaaact cttgtcgtct acttctaata tttcctgaca ctgtagcaag tacatttgat
    17401 aatccaatca tagccaataa acctgtaata aagcctacta ttaaattcat ggtctttatt
    17461 ccactagcct tttgtttttc actttctaaa acatcctcaa tactataatc tcctgaacca
    17521 tagtctttat tataaatgga ctctaatttt tctcttactc taggaatgtc ctcacgatta
    17581 tttactttaa atactcctat cagcctattt gaacgtaacc tacttacttc atcataattt
    17641 ttagatattt cctgcaccat agacattggc actatttgca ttaatgtata ttttgaacta
    17701 gtatctacta ttttaggaag tgtgcttgta atagctccca ctttcatgtt gtaagtataa
    17761 tcccctttat cttcgtcata tactttttca tttaatttaa tgttatctcc tattgaaaga
    17821 tttaaataat ctatatatac tttatttcta cgtgtactac gattaatatc ttcctcctta
    17881 ttatatacaa ttgaggttgg attttttgta tcataaaata actttgggtc tacagaaatt
    17941 tgcctacaat attctgtaaa actcttatca tcaagcccaa ttatatttga aattatacga
    18001 aacttgccat cacgttctat aggagagtat tttttagatg aaactatact cttaaatcct
    18061 ccatttttcg caagttcttt tgatgcatct ttttcagtca gccaagtgga agcagacatt
    18121 ttttgataat atagtgaact ttttacacct tctgtattat ctaatttttc aatgaaagca
    18181 gactctacag gttcactatt ttccaaataa actgctatat ctctttggtc cttataatta
    18241 tcacttccat aaacagcttt tgctccttct tgatttgcaa ttatatgcaa aaaacatgta
    18301 agtaataaaa aagataatgt taaagatatt gttgcagtac gataagattt tttacgagca
    18361 cgtagtgcgt ttgatgcaag ttctccttct attccaaata tctttcctaa tgaatattta
    18421 tgcgactttt tgattttaac actatctcct tgtcgtattg cttctatagg agatatcttt
    18481 gatacttttc gtgctggtat aagtgcagaa atccagactg ttactattgt aagtaaaact
    18541 gctggcagaa aagcaggaat accataagta aataccactt ctggaacatc agtatgataa
    18601 ttaactgaat taatataaac tataagccta ttacacaata accatcctaa aaataaacct
    18661 attggtaatg gtataattgt cagtaaaaag ccttcaaaca ctacagacct tttaatttgt
    18721 tttggtgatg caccaacact agataatatc cctaactgag taagtctagc atttgcagac
    18781 agtgaaaacg cgttgtaaat caccattaca aacacggcta ctgtaaaaat tgctattgta
    18841 agatacataa taggagttga aaattttgat atactactca tagatgcttt ctgttctggt
    18901 gaaaagacta acatctttaa aagataatta ctattgtatt taagattgta ttttccatat
    18961 tcattagtct tccatcccaa tgatttagca agttttggaa gttccttata cgtgtctctt
    19021 atatttttaa atcgcaagta cacagttata ctatcattag gttttatact tgttttatca
    19081 agatacccaa gagctgtata tgcaggtatt actgatgaag tagaaacatc tacctttcct
    19141 actattgtca atgtagcctc tgaaattttt ttaaaacttt cccctttcga ttttggggct
    19201 atgggttcaa gaggctttcc atttaggatt ctatctccta ttgggagagt aattttatca
    19261 ccaattttta cacttggatt attctcaaaa tattgttttg ataatgcaat ttcattagca
    19321 ttatttggtc gacgaccctc tattatagta tccttttctg gcatagaatt ccaatactct
    19381 gatgtagcat ttctataaat tatataatct cttttctcat cattaatttt gcctacatac
    19441 cattgtcctt ttaccattac atcatcaact gaagaaaaat tttcaattaa tggtaagtca
    19501 cttccataag tatagtcaaa caattcacca tgccagttac cttctttctc aattgaaagt
    19561 tttataaaat ctgtccacat ggtatacaac agtccacaca aagaagacat catggttgta
    19621 gtaagtaata tagctaacat tatagctata ctactacgtt tattgcgtcg tacaaagtct
    19681 aaggtgtatt cttgaagaat attcatttct tcaccacctt ttcagagaca atcactccat
    19741 cttccatagt tacaatacga tttgcctcta gtgctatttt ctcatcatga gtaataagaa
    19801 gtattgtctg attaaatcgt ttatttgata agtttaacaa gtctacaatt tcttctgaat
    19861 tttttctatc aagattacca gttggctcat ctgctaataa aatagctggt ctatagatta
    19921 aacttctagc tatagatacc ctttgttgtt gtccacctga aagttgactt ggaagatggt
    19981 ttaatctatc agataatccc aatatagata caatctcaga gaatctatcc tcatccacct
    20041 ttcttttatc aagtaacatg ggtaataata tattttttct aacatcaagg gtaggtataa
    20101 gattataaaa ttgataaata agacctactt ttctacgtct aaaaactgca agagcttctt
    20161 cctttaaact tgatatttct gtatcttcaa cataaatctt tccagatgta ggtctatcta
    20221 ctcccccaag cagatgtaac aatgtagatt tgccagaacc agatgctcct acaatggata
    20281 caaaatcacc tttttgaact gataaattta cattattaag ggctgtcact ctggtttgat
    20341 ttgaaccata tatttttgtg agattttcac attttaatat ttccatatac aatcatcttc
    20401 tttatggtta aattttagca acctaaaatg acattatagt gacatgaata caaatataaa
    20461 ttactattaa aaatattgtt tagtttttgc acattattta aaattataat ataaaacaaa
    20521 aactatgcaa tatattttat ttatactttt aatatattac atagtcttta aaaggtatta
    20581 actttaaatc tataggtatt caatttaata atcaatagca agtattttat gtactaaagg
    20641 agttttgctc tatatagatt caatttagta aaattattta ataaatatta cagagtatag
    20701 gtaaataata tttacaaata aaattaagtt tattccctta cactataata tttcttaatt
    20761 atattaattg caatttcctt tacctttggc cctgtaattt ctttattgga atcatctgat
    20821 ttaatagcaa aataataggt gtcaccttca cgctctagca taccaacaaa ccatgcatcc
    20881 acacgtttat ttttttcatc aaatcctgtt ccagtttttc catatacatt tatgttttta
    20941 tcatttacat ctattttcat aatatctctt aaaatattaa tatgctcttt tttaaagttt
    21001 gtatatccct caaatatttt tgccattgtt tgcacttgtt cttttggaga tatttgtaat
    21061 gaagattcta accaaaatcc atttaaatgt ccctttccat tttttaaatc tccttcccat
    21121 tcgctaatat cgcaattccc atactttaaa tcatctaaaa tgttttggac agatttagca
    21181 tcaactttat ttatcaattt tttgtagtac caaacacaag actccttaaa tgcctcttct
    21241 aaactaagat ttttattcca atttttattt ggatactctg taccatcata tcccataact
    21301 gattcttttg agtttattac ccctttttct aaaccaatta aagttgaaac tattttaaaa
    21361 gtagaacatg gtgaccttct tgtttcaatt aattctttgt tatatatatt atattcttta
    21421 tttttagtat tataaaaaat agctcctcca ctaatacctt caaaacaatc actatagtca
    21481 accatattaa ttttttcttt tttgttttta ttattgtttt caattttgga tttatttaat
    21541 tcagatttat ttaattcaga ttttatataa ttcttatcag ttttttgatt tatatcgtta
    21601 tgttttttcc ctacataata cattaccata actacaccaa ccaataagat agctatccaa
    21661 ataaaattct tttttctttt cataatttat atcatctccc atatttaaat ttataaccta
    21721 ctattaaaat agtagacatt atttgtaata tactccaatc taggttctac ttataagaaa
    21781 caagactaaa tgaatagctg tatctcttat attttcttca cctagtacaa ttatgttgat
    21841 taaacctatt gcgaagataa ttaggtaact attaataaca tcctgtcctc cttttaattt
    21901 gtgatataat aattgattcc acatttaaaa cttactattg taatatttaa atattacaat
    21961 agtaagtttt aaatgtcaag ttagtttggg aaagttattt taattgtata attgttatta
    22021 catttataaa atatggcatg tttaaaatta ttttgctatt gggaggatta agataagcag
    22081 tattataaaa gctataaatt ataaaattta atatataatt tatagctttt acttttacaa
    22141 atattgtttt tattgattta ttaatgaatt ttattgacag gttatattta tataagtaaa
    22201 cacttacata ataagtatat aatctacata tctataagtt gagtcaattc accagtatat
    22261 gtcaacttaa gtttatgcat agctcgtgta caggcaatat ataacaaact gcggtcataa
    22321 tcactataat atgttttatt atttactgaa ggtacaataa cttcatcaaa ctctaaacct
    22381 tttgacattt taatagaagt tatggatact ccttttgtaa atctagaact ttcagaagat
    22441 attaagtgaa cactatattc ttctcttaac gcatcatata cagctttagc gtcattatct
    22501 gtctttaata taataccaag agtcgcatta tcactctttt taaattcttc aatttctatt
    22561 tttatcttat ttatttcatc ttgctcgtta ttacatttta ataaagctgg ttcttcacca
    22621 tgtcgttcaa taggctctaa tgaagatatg tcttgaatct ttttggcgaa attaataatc
    22681 tcaaaagtag aacggtaact cttattcaac ataactagtt ccccatcatt atatagctgt
    22741 ttcatatcat ctagtgtatg agtatgatta ggattaacca attgtccaaa atctccaaga
    22801 atagtctttt tacacttaaa aagtaagttt attacagcat attgaatagg tgtatagtcc
    22861 tgcatttcat caataacaat atgccttata atcttatctt cttgtatacc ttcatatgca
    22921 gcatgaatat atataaatgg gtatacatct gaccattcaa gtgttttctt ttctgccatc
    22981 acaaacatgt ttgatatatt catttgttta aagaaatcct tatatagtgt aagagtattt
    23041 tttattgtca acattccatt caaactttta agtatacttt ttacctttgg taaatcttct
    23101 tccataatat tatctgattc aaatttataa tgaatgtctt cagctacctt ttctaatctc
    23161 ttcttaactg gatatctgtt atatgcctca aatctactct gtatccagtc acttttagct
    23221 gtaaaacttc caaaagtgta gtcttttgga ataaaaatct tatttggcat ttgttttata
    23281 taatcatcaa taagttttac aaaatcaagt gtagatttaa aacgcactct ttcagaccat
    23341 ttagcatcat ttatttctaa tgggtctttc tcactttcaa aattaataac tctatctaat
    23401 tgtacctctg ctatattttc aaaacttaat tcacataatg gctcttctcc aagctcagga
    23461 aggacattgg aaatatagtc tccaaagact ttattaggtg ataggataat tacattattt
    23521 gctgatattt tatctttaaa tcgataaaga agaaaagcaa ttctgtgtaa tgctatagat
    23581 gttttcccag aaccagctac cccttgaata atcagtgtat cagctttatc atttctaata
    23641 atttgatttt gttccttttg tattgttgaa atgattgact tcattttttc atctgaagtg
    23701 tgactaagtt ctttttgaag aatatcatct tgtatattaa tggaactttc tagtgcatat
    23761 tctaattttc catttttaat tttaaattgt cgttttcttg ttatttctcc atccacccat
    23821 cctattggag catcatatcc agcttttccg atttcataat cataaaacat actggcaatt
    23881 ggagaacgcc agtcaagtat tataagctca tcttcataat caaatgcaaa tctaccaata
    23941 taatacttag attcatcatc catatctttc agcctaaaat ctattctagc aaagtatgga
    24001 gaatctttga ttttattaat cttatctcga acttttacca taaaagcccc atagttatct
    24061 atttgtttta gtccttgttc attttgaaac atttcatgtg ggtctatttc accacgattt
    24121 tttaccatat acattttagc gtccatataa tccttatcaa gcttgtcaac aagattttca
    24181 gctttcttaa aagcattgtc tagcttgtta tttatctcta ttagatgtgc aatctcatct
    24241 ggaaaaaaag ttaataactc tgcatcatat tcacacatat tatgtttaga attagatgca
    24301 ataatatcgc aatttactac ttgtttattt tcagacattt aaatcctcct ttgttaatta
    24361 agtacttaca ctgaatcatt atacttaatt gtgttttgga gtttttaatc attcttgcta
    24421 tttaacttaa aggaagaata aatttttgag taggatatcc aaattccaca agcaactctg
    24481 cttctgcaaa gcccaacttc tttatcatat ctctatgtcc tgtatcagct ttgtcaccct
    24541 ctcgaaaggt agtaattgaa atatctgtat ctatcaagaa atcttcaata actttcttca
    24601 aaaacacttg tgcaatgtct gaattacgat actgtggatg tattccaaaa aaatcaatac
    24661 ttctagtttc tctattaaac accatatttg caatagcgac attattaagt tttaatatta
    24721 aagctctttc ttctaaaata tactgttcta attgatatat atattcttcc tcttgtaaat
    24781 ttggaaatcc atctatgact aatctcacta aattcatcca taatggtatg tcagacatac
    24841 ttgcaagttt aatttctttt tccaactcta gtacattctt tgaaaatagg ttatcttttt
    24901 tcaaaacaaa tcttagctgc aaaggataaa attcttgttc tttcctatat ttatttggag
    24961 attttttata catagcttta aatatagaag tgaatgcctg ttgactttca tatccagcta
    25021 tcaatgcaat ttcaataatt ggtttctttg aaaataccaa taattttgct gcttctgtca
    25081 actttctccg ctttatgtaa tcatgcattg ttaggccaac agaagttgtg aaagttctat
    25141 gcaggtgata ttttgagtaa tgaacagcat tagcaactat atctaaatcc agcttttctg
    25201 ataaatgctc ctctatatag tcaatagcag ttatgacatt aattactttg taagacattg
    25261 cagcttccct ccttttatcc atgcaacatt atatcaatta ttacttagtt atatttaatg
    25321 aatcttgcta ttttataaac aaatttgttg tttaagtttt caattcataa atttaatata
    25381 ttacttttta ctttctgatt aaagactcta atcctttaat tttcaataac ttttcattta
    25441 gtaaatctaa aaagttattt attattttct tcttattaga ttccttttag attccttaat
    25501 taaatgataa atatttatat tatacgacta aaaacagctt tttacaatta tttaatatga
    25561 ttacaaactt accctatctt aaagtttaat ggggtcctgt tatgaatctt tttattttaa
    25621 tttaagctct tcatcagttt attttatata ttgatgtatt tgatttcata caaaaaagag
    25681 tgcttcatga actaaaaagt tcatttttaa acactctctt ttatgtaaat aaatttctat
    25741 tctattttga aatattataa tttgacttat aatctattaa taccaaaaat aataaattgt
    25801 acgtctaaga atagattcta attagttgct cagtagtaaa atctattttt aactaatcct
    25861 gctttgtatg ttgaacatat tctataattg taccatctgg atgtctcaat gtcatattat
    25921 atcctgtagg aacttttttc ttatctctta ctacaactgc tccattatcc aatagataac
    25981 ttctccattc atcaacagaa tcaaccataa aagtagattt tgtttctata aatggtttaa
    26041 gagcatcatc agaaccagat aatagtaaaa catttcctat ttgcgctaat tccaacccaa
    26101 cttctttata ggaaaaacgc aaagaacatt tttcttttaa aagattctca taaaaaggta
    26161 tggctttttc tatatcttga atataaaaac gtgttaatat atcatatatt ttcatatttt
    26221 ccccctatat atctatataa tattacctct aaattatacc actctacaaa ttatataaca
    26281 ataaattaag catagccttt tgagcatctt aagtagacct taacaatata ttggggttac
    26341 attagaataa taaattaaat ctaaattaca ctaagttagt tataaattga ttatctacag
    26401 ttacattaga atacataaat taaatcacct tgatattatt taggataaaa tttatgcagt
    26461 agcagtaaat gttcttctaa ttatcaacta acaaacgata atctttaact ttctatataa
    26521 tgggtaacat cattttcgta agatattcac tagctggacg attgatatca tttaaatact
    26581 ggtaatatat atcgccatga agtttatatc cattgctatt tgcccatgaa aataagtctt
    26641 ctaaagtagg gtcttgtaat tcataaggtc ctaaatcaat tgcagttatt attttctgtg
    26701 caggaacaat cctttgtaga atctcatttt tcccatctat ataagtggct acaggaaacc
    26761 ctacttcaac atccaatttt gctaaatcca tgttatgaaa acatacaatc ggtgctcctg
    26821 aagataaaat tcctctctcc tccagatatt tagaaatttt ttcaaaactt tgttttgaaa
    26881 actctgaaaa ctctaccata aagtcaattg tttttcgtat tgccaaaaaa cagtactcag
    26941 attgttcagc tattgttata ttggatattc tagtcatttt attcctccat tactttattt
    27001 tcatttctaa tctttttata ccaaattata cgaaaattta ctgtgtaact aattatttca
    27061 tatgaatata aaacataacc tccttccatt tcttatatcg aaagtatagc aaaaataatc
    27121 tcatcattct tgtcacacag tgctcttata atcagaacat acttaaaaga cttgcctaat
    27181 ttttaggcaa gtctaaaaga aatctttttc tataaaaaat ttatacaaac aattaaaata
    27241 tcaaaatata gactaatata aaactataaa tttactaatc tgaacctccc atgactaaag
    27301 tcacaagatt actgatttat agaatttcgc acactaaaat atgtcttact aattccccac
    27361 aggctacccc cctagttcct acgttcttac ataagttatc tattatcaaa catcaaaatt
    27421 taaacaagtt tcaggttcat cttctattct tgctatttta aacttacatg caattaatac
    27481 taaagatact attggattta taatatttaa taaagcgtat ggaccatatg ttagtgcaga
    27541 tacacctaat gttgctgtta tatacgcccc gcatgtattc catggtacta atggagaagt
    27601 taaagttcca ctatcttcta aagctctcga caacatctta ggatgtatac ctctcttttt
    27661 atattcctta ttatacattc ttcctggtat aactatagat agatattgtt cacctgctat
    27721 tatattggta attgtacaag ttaccatagt acataatact gttccaaata caccctttgc
    27781 taatcttagt aaagaagatg ctactacttc aagcatacct gttttttcta aaatacctcc
    27841 aagagataaa gcgcatattg ttaaggatac agtggacatc atattcataa tcccaccttt
    27901 agataataaa gcatctacct cctgtatttg tgttgacgaa acatatccat tttgtgctgc
    27961 atctaatatc gtattcatag attctccttg aaaaactact gcaaacaata ctcccaatac
    28021 agcacctact ataagccctg gtatagcagg aactttaaat acaaccaatc caattactat
    28081 tgctggagct aataataata ctggtgataa agtattaaat gtagaatcta atgtagacct
    28141 aattaattct atttgtttta tatctagtgc ttgacctgaa tacttcattc caattacacc
    28201 ataaattact aaacaaatta ataatgatgg tatagtcgaa tatagcatat attttatatg
    28261 tgtaaacaaa tctgttcctg ccattgctgg agctaagtta gtcgtttcag ataaaggtga
    28321 tagtttatct ccaaagtaag ctccagatat tatagaacct acgattaatc caattggcat
    28381 acctaaactt tgaccaattc ctaaaagagc tactcctact gtacccattg tgctccaaga
    28441 tgaacctgtc gcaagagata ctattgcaca gactaatgtt gatgctacta aaaaaatatt
    28501 tggtgataaa atttttagtc cccagtaaat catacatgga actactccac ttactatcca
    28561 agttcctata agtacgccta ttataattat gattaagatt gcttgcattg ccatctttat
    28621 actattaaac atacttaatt ctaactcttt ccatttaaat ccaagtctat atacagccac
    28681 aagtcctgct actgctgctg ctcctattag tggtacatga ggtgatgaac catacactaa
    28741 tactcctaga cttaaaaata atatcaaaca agtaattggt atcattgcat caaacagact
    28801 tgcttttcta cattctcgct ctttcataaa atagtttcct cctaattatt taaacactac
    28861 actataattt tttttgaaac atatttgctt gtactagatg acatgatacc cccataagtt
    28921 aataaaaatt ctaaattgtc acctaattta taatcatatt ttgtgttact tacatctagt
    28981 atcatgtggt ctgaacttgc ccctaatatt tttatatcag tgtctattgg aattaaagag
    29041 tcaatattta tatcttgttt acctatagct atgattgccc tttttaagat tcccttatct
    29101 tcataaacag gtttatttct aaaggcatca actcctattt ctccaatagg aactgatggt
    29161 ttttctttac attcaactac ttggcatatt aatttaaatg catcttgata tgttccattt
    29221 atattttcac cgtatgccgt ttctctacca agtagaatgg actcgcctat tctcaaatta
    29281 gtaatacctt ctggcatatt attatttatc aacagatgaa tcgaacttga attacctcca
    29341 gatactattt ctagatttat tctaaatttt tcttccattc tttttgctat acttaccagt
    29401 ctgcctatat tttccctaga tggtataata gctccataac aggttaagtt tgtagctata
    29461 ccaattattt caatattttt taatcttact atctctctta cattactaaa aaagtcttct
    29521 tcataaaagt atccttctcg taaatcgcct aagtcaaaca taagaactat cttatgaata
    29581 atatttttac tttcacataa ttcatttaat ttctttattg ttcctaattc tgagttaaaa
    29641 ctaatatcgc aataattgat aacttcttct aattcactta acattgggat tctaatcaat
    29701 atcttaggta gatttatatc ttgtaaattt tttaagtttt gtattcttga atctgctata
    29761 tggtctattc cttcactaca tatttcttct actatttctt tttgtgcaca aaatgattta
    29821 gtaacaaaag atatcttaat atttctttca tgacacatct gcgaaattaa ttttgcatta
    29881 tgctttaact tttctatatc tatttctaac ctaggataca taatctttcc tcctcaaaat
    29941 tcaaactttt taacaggagc ttaagagatg cttcttcata ttttttagat aaaacaccca
    30001 tagatgccat aatatagtca ttatctatta atatctttgt tttttccttt ggaaatagta
    30061 attcattatt tttattgtat ttgagcatat tatctaatat ttcaattcta ctagggagcc
    30121 tagtaagtgc tccccctgta ccaactatat atcttacttc tgttaaatct ttcccttctg
    30181 caattttgac ttttccagtt gttccataga tatttcttat ttttcctgaa tgtcttagca
    30241 tagctttaac aacagcttct tttgtcaacc tctcaacaaa taatatttct tcttttgatt
    30301 ttggtattgg aggataattc tctattactg catcaatatc tatagatagt tcactttgta
    30361 agttttcttt acctataact tctactatat tcttcatatt tacatataca cctaaatccc
    30421 cctcaacact tctctttgca gtaggttcag gattaaccaa aattttatta atatagtcac
    30481 ttccatctgt aacagaatgt acatcagtag ttgctccacc tacatccaga gttaataaat
    30541 ctccaatatt tttatataaa agtttagaag cttccattac agctcctggg gttggagtaa
    30601 tattttcatt aaccatttct ttaatgtatt tcattcctgg tgctgtagtt atgtgttctt
    30661 caaatacact ttgaatgatt cttcttgtag gttctatatt caataaatct attttagggt
    30721 atacattttc agttatataa agcttataat tagtatcttc aaatattaac cttatttcct
    30781 cttgattttc aacattacca gcatatataa ctggtatgcc tagattcata gaagctatca
    30841 tttctgcatt ataaatagct gtatctcttt ctccataatc gactccacca gctattaaaa
    30901 ttatgtttgg gtttatttct tttattttat ttaaatcagt tcttttaatt ctacctgatg
    30961 ttatttgacg aataacagct ccagccccta aagctgcttc tttagctgct tttactgtca
    31021 tatcatagac taatccatga acagtcattt ttaatccacc agctgcacta gaagttgcaa
    31081 acatatcatt atactctata tcatctacag ataatttatt agctaaatct ttaatagcac
    31141 ctgataagcc atttcttaca tcgccacctt caaacactgt tgttggagct tgaccttgac
    31201 ccaaaaatat aggattgtta gtattaatat cgtggaaagc gtttaccact gtagtagtac
    31261 ttccaatttc agctaccaac acatctattt ttaaagaaac tttgctattt tccattattt
    31321 attactcatt tctcttcttt tttcaactaa gaaagtggct acatgtatgc ctttgctgtt
    31381 tcttccaaat ccagcatcta tgccttgttc aacagctatt tcaggtgtta cttgtgttcc
    31441 tccacacgca atcattactt tatctcttat acctttttct acacaataat cgtgtaattt
    31501 tttcatgttt ttatagtgaa tatcatcatg acttattatt gtagatgcaa gtattgcatc
    31561 tgcattcagt tcaatcgctg catctaccaa tttttctaca ggtacagaag tccctagata
    31621 gtggcattct atgccatatt tttcaatacc tccatgtttt atatctatta tttctcttaa
    31681 accaactgaa tgttcatctt ctccaaccgt agctgaaact attttcatag gtttttcttc
    31741 tatatctgct cttatttctt catcagttag taccttaggt tcttctggta ttactaaatt
    31801 gtttaaatct atatcaaacg gaactcttcc tttcacttct attctagtcc cttcagattc
    31861 atgcataact tctctactta tgacctcaat ttcttgtaag ttcatttttt tgacaaattc
    31921 caaagctgca aactctgctg ttcttttatc agtaggaagc atcattgtta tcattacaat
    31981 tccatctgca caccattcca tttcaggctt aattttagtt ccatttctgt actcatatga
    32041 ttcttttagt ctattttcta cattatcaaa atcgtcaagc tcatcaatat atattatttt
    32101 gtctggattt tcaaatgtac atccatctat taacattgat ggattatcta tagcattttc
    32161 atcatattga gctacattat tgtatccaaa gtgagcagtt acaggtgcta aataatcttc
    32221 ttctctttca taaacagtac cttcaccaac tccacctttt atttttctag atattccatc
    32281 accatttctt tctggataat ttccagaatc aacaaaaaat ccttgttcca cagattcaaa
    32341 atatccacca acttcagata tttcttccat atataataca gctctttctt taagttctct
    32401 tgctttgtct cttaattcac caacatcttt aagttctatc atatccataa gcccatccat
    32461 accaactaaa gcttgctttg ctgtatcaca tgcttctata ttgtatatat gccaaggtac
    32521 atttctacct tcatcaggtg ttatagttga ttgtatgtca gctctagtta atactgatgt
    32581 taataagttt aatacatgag taactgttgc ttctctagtt gatgattcca tatatttagt
    32641 attcatttga gctctcatct tataatcact aaataactct ctaagtgcaa cagcgtaagg
    32701 taagtctatt tttaaacatg gtgctggagg tgctgttggt ggaactgttg ataagcatat
    32761 attactttta tctataccta ttttttctga gaatatagag tttaaagcgt gttgaaccat
    32821 aagttcaggc attaccttcc atgcttctct agcagtagca ttagcattat gtgctccatc
    32881 aatttgagct atattagcaa aagccattat tttctttgcc tcacaagcat ctacaaaaga
    32941 ccttaccatg tttatattcc tatacagaac attatattgt gggtcttgat gtgctccatt
    33001 gactccttcc tcagcaaaca ttactgctac ttcaggacca gcaacaccac ttacatatga
    33061 atggtaatta ataggtctac caacttcttc ttctataaaa tcaagagcct tcctttgagc
    33121 tcttacttgt tttcttgtta ttggaactcc acctatacct tgtggagttc cttctataag
    33181 cccatcaaaa tgtgattgtc cagcagttct gataaccatt agatggtcag ctccatgatg
    33241 agctgccatt ctcattcttc ttatatcatc ctcaaatcta ccagaagcta tttcagtagt
    33301 aataactgga cttggttgag ggtctatatt attgaaatac ttagatgatg gtagccctac
    33361 actcttcttt aaaggttttg tacagtcttt atatttaaaa ggtcccattt caagattttc
    33421 caagtgttct ctccaaaccc aacctcttcg ttttggttta tatttatcca aatcttttaa
    33481 tatatagtct atatccaatt ttttattttc ctttaacatg attctacccc ttctctaaat
    33541 aaacatgtaa cttcatccca atattcaccc ttaactaata acaaacctgc ttctctaacc
    33601 gttatatttt tagatttagc caatttataa actacatgcc ctgctccttt acctataaga
    33661 cctctttcca taaccccttc taaaataggt tttacttcaa gagaagaaaa tcccattctc
    33721 aacaagacag acctttctat tgatggtgtt gtattctttt taccaagttc tatcattggt
    33781 tctactatct gggtagctaa actccaaaac ctttctttta actcatcatc actcagttct
    33841 tgaagatgtt ttcttctaac ttcaaaatca tcctctcttt ttttcatgaa cattcctcca
    33901 ttcgtattga tttaatcgaa ttttctgaat ttttatttta tattaaatta aaaaacaata
    33961 attactataa attatttaat acatcaacaa caaaatctct tgaacttttt acttcttcca
    34021 ttaaaaattc tatatctcga ttacttagct tatcaatatt atcaatgtga ttattagata
    34081 tacaattttt tataaatgac tttcttattt tattcatatc aatatcaaca catttcaata
    34141 attctggtct ttctggtaat attaagttta ctcctgcaat ttcttctctt ggatttccaa
    34201 attttatatc tattccattt tctcttgcaa aggtaagttg tggacatata tgtttacctg
    34261 cacccgtata ttctgtttct tgtaccacta tagctttatc ctcatctagc tcttgagcta
    34321 aagagaatgc tgctgctaaa gatgtatttc ctgctggacc tctttctata ccttctagct
    34381 gtgccaataa ttcagttgta taaaaaactt ctccttgttt tactaataca tatctatcca
    34441 tatatctaag tggtctagct gcagacctag gtacatctga cctatcaggc catgtacagt
    34501 atggtatccc aaaacctgta tgtcctgttg taaatgattt tttattaaat tgattatcac
    34561 tagccatgtg taatcctttt aaatctacac ttgctcctac tactttagta ttaatagaac
    34621 cagcttttat caatcctctt gcagttccag taagattacc accacctgca tttgtacaaa
    34681 caactatatc agggtctttt ttatactttt ctctaaattg gatagctaac tcataaccta
    34741 aagtttctac acctgcaatt ccaaatggtg aatataatga tgcgttaaaa tagcctgttt
    34801 cttctaataa aactaagaac gtataaaata actctggtcc aacagttaac tgaacaactt
    34861 ctgctccata tgcctcacat tttcgtgctt tttctataat ttcaggttga cctacaccat
    34921 tagaatcata acattcttga actattatac attttaatcc ctgcattgcc gcttgtgatg
    34981 ctacagctgc accatagtta ccactggtag ctgctattac acctttatat cccatttgtt
    35041 tggcatgata tactgcagta gctgctcttc ttgctttaaa actaccagat gcattcattg
    35101 cttcatcttt tacaaatatt ctagctcctt taccttcttt agcacatttt ctagctaatt
    35161 tagtcaaatt tttaagctct attagaggag tatttccaac agcatactgt gattgaattg
    35221 cttcaatatc ttgtaatgta tatcctgttt cactcatcat tttctcataa tcaaacccta
    35281 ttccctcttg ttcaaaagaa gaataatcca atccgattgc atttttcatt atttcgttat
    35341 tttttcccat aacagcttga taactcatat ctttcattga tgttgaatta ttcattatat
    35401 acacctccaa ataatatttc tttagcttgg attcctattt ggagtaactc tggtatacat
    35461 ttaccataat catgagtata atatggattt actttaagta aattaccttt tacaagtcta
    35521 cctataattg ttttaacttc tactaagtcg cctatagaag catcactttg tataaaacct
    35581 ttaacccaca tttctaagga tactttctta gtatcctctg gaacttgtgg agctctttct
    35641 tctggagtca atactatatt gtgtataata acccaatcat taattttagc atccattttt
    35701 tcatcccctt attaattgac tattgcctta ctaattaatt tactgttatt ttaaattaaa
    35761 cattatttta ccaattagct aactgttatc ttactaatta gcctttgatt cttcttccaa
    35821 ttcaacttct atctgatttc ttatatctcc cattattgct ctaggtactg gtatatctaa
    35881 catagtttta agacctggtc tagcatttat tattaatgga atagagttta ctatcattgc
    35941 ataagtacct actcctccag gtatttcagg atttatttgt aaatcaatgt ttggtatacc
    36001 ttttattgat acatagtctc ctgttttaat accttctgct tctggtatta tctgttgtgg
    36061 atgctccatt tcaattaaaa cttctccatt tacatatcca tatccacatt gtctacaacc
    36121 agcaacattt ccagccaatg cctctccgta cttagatttt ctatctactg tagtcattat
    36181 tggctctttt gtttgttcta tcttatctaa tttccaacct aatccatcag ctatcatatt
    36241 tattgattct acaaaaccta catgacctgc tatagtctta tcttctacac cttttataaa
    36301 ttcttccctt gttactccaa caccttgttc caccataaca gactttccaa atggagataa
    36361 atcatttact ctcttagctt ttatactagt aacttcttca caagtacctg aaagtgccaa
    36421 tactagtaaa tctaatacaa atcctggatt tattcctgtt ccaagtatac ttactccatt
    36481 ttcctttgca acttcatcca tttttttagc taaatctgca tcatcagctt gtggatatgc
    36541 catttgttct gctgttgata taacatttat ttttctattt aataagaaaa ttattttatc
    36601 aaaagccttt ttagtaaatg aatcagttgc taacaaaaca acatctacac ttttttctct
    36661 aaaaacttct tcataatttt tattgataat aacttctggt ctttctcctc tttcaattcc
    36721 taaaacatca tacatacttt tccctgaagt acttctacta caaacagata ctatttctat
    36781 accttctttc tttagaatca tattagccat acctattccc atagctccaa atccccatat
    36841 tcctactctt acttttctca tatatacacc ctcctaaaaa tttatatatt ctcaataatt
    36901 agttaaagca taaatcgtgc caactttaaa attaaagcaa atatttattg gaacgttgaa
    36961 actataatta ttttattatg tattttcata aataaaaaat aatatttctt atgagaaata
    37021 aaaaaaagag caaaatattt tgctctttta taataatttt gctcctctat tttgcttttt
    37081 tattttactt tgattttgtt tttttatctt gcttatttat aatattttga tattatattt
    37141 ttttatttta tactgtaaat tttgcctatt catacctaaa attgatgcag ctttagttat
    37201 attgtacgat gccttttcta atgattcata tatatacttt tcttctatct cactgataat
    37261 cttactaagt gttttgtcct ccaaatcgtt taaagaataa ttgttactct ctttattctc
    37321 tttaagattg taatagtcgt ttagattaat catacttgtt tctatttgta cctcatcttc
    37381 catcatactc atagtacaat aaattatgtt ttctagctct cttatgttac cttgccaatc
    37441 gtaatttttt aaaattttaa tagcttcatc tgaaattcct accacttttt tatttaactt
    37501 tttattatac tttgaaataa attttttgca caatagaggt atgtcctctt ttctatctct
    37561 aagaggagga atttttatat gaattatgtt aagcctataa tataaatctt ttcgcatttt
    37621 atgctcgttt aaaattacct ttggactttc atttgtcgtt gcaataattc ttacatctat
    37681 aggtatatcc ttagttcctc caacccttct tacataacct tcttgtaaaa cccttaatag
    37741 tttagcctgt aacataatag gcatggaatt tatctcatcc aataacaatg tccctttgtt
    37801 agcttgttca aataagcctg gcctatctat tgctcccgta aatcctccct tgctagttcc
    37861 aaaaaggata ccttcgaata gtgattccgg caaagctgca caattttgtg ctataaatgg
    37921 atagtttctc ctattacttc catagtgaat ggactggcta aataactctt tacccgttcc
    37981 agtttcaccg tatataaata ctgaagcatc agacttgcaa gctttcttag atttttctat
    38041 tgcttcttta atcaaattac tttcccctaa aatatcatca aattcataac cctttttttg
    38101 tctactacta actttattga ctaaattttt tttataattt ctgtttagta aattttctat
    38161 cgttgtcata ttctttgata tttcaagtgc ccctacaaac tcgcctttaa tatcaattgg
    38221 tattgtagta ttaattgctg ttatctcttt tccattttta tctagatatt tttgaatatt
    38281 gtctttgata acttcccttt tttccaaaac ttctaatagt gtactcctgt ttattttcat
    38341 tttgttgact atatcaaaaa atgaattggc taaaatatcg tgtctgttca ttttttccat
    38401 tttttgtaca gaattattgt agattatagt tttcccgtct ttattaacaa aatgtacccc
    38461 ttcatcaata ttttccaata tttcattcat tattttaaaa aatatctctt ggttcaacta
    38521 tatcacccca gtacctaaat atataaaaac ccaaaaaatt aataaaatgt tgctattgta
    38581 tttatcttat catatattg
//
LOCUS       DACXMZ010000010        38297 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.010, whole genome shotgun sequence.
ACCESSION   DACXMZ010000010 DACXMZ010000000
VERSION     DACXMZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38297
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.010"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1091)
                     /locus_tag="JD016_01985"
     CDS             complement(<1..1091)
                     /locus_tag="JD016_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAU4998399.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSI"
     gene            1243..1330
                     /locus_tag="JD016_01990"
     tRNA            1243..1330
                     /locus_tag="JD016_01990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1277..1279,aa:Leu,seq:caa)
     gene            complement(1517..1960)
                     /locus_tag="JD016_01995"
     CDS             complement(1517..1960)
                     /locus_tag="JD016_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998400.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(1963..2160)
                     /locus_tag="JD016_02000"
     CDS             complement(1963..2160)
                     /locus_tag="JD016_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4998401.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(2352..3218)
                     /locus_tag="JD016_02005"
     CDS             complement(2352..3218)
                     /locus_tag="JD016_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAU4998402.1"
                     /translation="MEWINKLNQAITYIEENLKSGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(3444..4313)
                     /locus_tag="JD016_02010"
     CDS             complement(3444..4313)
                     /locus_tag="JD016_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HAU4998403.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(4819..5040)
                     /locus_tag="JD016_02015"
     CDS             complement(4819..5040)
                     /locus_tag="JD016_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998404.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(5541..6521)
                     /locus_tag="JD016_02020"
     CDS             complement(5541..6521)
                     /locus_tag="JD016_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998405.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRNLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(6685..7557)
                     /locus_tag="JD016_02025"
     CDS             complement(6685..7557)
                     /locus_tag="JD016_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAU4998406.1"
                     /translation="MIIKVSMTVLVLILLYILYKYEIQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(7721..8272)
                     /locus_tag="JD016_02030"
     CDS             complement(7721..8272)
                     /locus_tag="JD016_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU4998407.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(8292..9194)
                     /locus_tag="JD016_02035"
     CDS             complement(8292..9194)
                     /locus_tag="JD016_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAU4998408.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(9209..11581)
                     /locus_tag="JD016_02040"
     CDS             complement(9209..11581)
                     /locus_tag="JD016_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HAU4998409.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(11802..12491)
                     /locus_tag="JD016_02045"
     CDS             complement(11802..12491)
                     /locus_tag="JD016_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998410.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(12732..14111)
                     /locus_tag="JD016_02050"
     CDS             complement(12732..14111)
                     /locus_tag="JD016_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAU4998411.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEMCLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENVNKYIRIKGTMTRSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     DQDTDSWGD"
     gene            complement(14105..14818)
                     /locus_tag="JD016_02055"
     CDS             complement(14105..14818)
                     /locus_tag="JD016_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4998412.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLSKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(15069..15629)
                     /locus_tag="JD016_02060"
     CDS             complement(15069..15629)
                     /locus_tag="JD016_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HAU4998413.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(15724..16527)
                     /gene="zupT"
                     /locus_tag="JD016_02065"
     CDS             complement(15724..16527)
                     /gene="zupT"
                     /locus_tag="JD016_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAU4998414.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(16678..17841)
                     /locus_tag="JD016_02070"
     CDS             complement(16678..17841)
                     /locus_tag="JD016_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAU4998415.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(17901..19088)
                     /locus_tag="JD016_02075"
     CDS             complement(17901..19088)
                     /locus_tag="JD016_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998416.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(19451..21151)
                     /locus_tag="JD016_02080"
     CDS             complement(19451..21151)
                     /locus_tag="JD016_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4998417.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(21394..21669)
                     /locus_tag="JD016_02085"
     CDS             complement(21394..21669)
                     /locus_tag="JD016_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU4998418.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(21788..22321)
                     /gene="mobB"
                     /locus_tag="JD016_02090"
     CDS             complement(21788..22321)
                     /gene="mobB"
                     /locus_tag="JD016_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAU4998419.1"
                     /translation="MYNILSVVSYSGVGKTTLIEGIIKELNKKGYRVGVIKHTCHDFD
                     MDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQLYEDMDLIIIEGYK
                     NYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLNDYKNISKYIESCIK
                     NEILSISNVEIKNILKE"
     gene            complement(22344..23525)
                     /locus_tag="JD016_02095"
     CDS             complement(22344..23525)
                     /locus_tag="JD016_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAU4998420.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            complement(23736..24470)
                     /locus_tag="JD016_02100"
     CDS             complement(23736..24470)
                     /locus_tag="JD016_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998421.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTREKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKSMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            24832..25737
                     /locus_tag="JD016_02105"
     CDS             24832..25737
                     /locus_tag="JD016_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAU4998422.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(25897..26739)
                     /locus_tag="JD016_02110"
     CDS             complement(25897..26739)
                     /locus_tag="JD016_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAU4998423.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(26723..27487)
                     /locus_tag="JD016_02115"
     CDS             complement(26723..27487)
                     /locus_tag="JD016_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAU4998424.1"
                     /translation="MKMEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALG
                     NLHTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPI
                     ATLVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGF
                     SLGVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVA
                     YLNITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            complement(27498..27968)
                     /locus_tag="JD016_02120"
     CDS             complement(27498..27968)
                     /locus_tag="JD016_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU4998425.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(27968..28711)
                     /locus_tag="JD016_02125"
     CDS             complement(27968..28711)
                     /locus_tag="JD016_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HAU4998426.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            complement(28715..29128)
                     /locus_tag="JD016_02130"
     CDS             complement(28715..29128)
                     /locus_tag="JD016_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAU4998427.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            complement(29154..29879)
                     /locus_tag="JD016_02135"
     CDS             complement(29154..29879)
                     /locus_tag="JD016_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU4998428.1"
                     /translation="MDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLPSEGDLCKEF
                     NISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSCHRINAKPST
                     KIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRIDYMFLLKEE
                     LEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKVRQSVYTENN
                     DVLYYNEQFIRSDKYKYAVSAEI"
     gene            complement(30146..31540)
                     /locus_tag="JD016_02140"
     CDS             complement(30146..31540)
                     /locus_tag="JD016_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAU4998429.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            complement(31560..31769)
                     /locus_tag="JD016_02145"
     CDS             complement(31560..31769)
                     /locus_tag="JD016_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HAU4998430.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            complement(31798..33252)
                     /locus_tag="JD016_02150"
     CDS             complement(31798..33252)
                     /locus_tag="JD016_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAU4998431.1"
                     /translation="MINLEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPI
                     RKLSNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQR
                     EDLIHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSD
                     GNIKTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISI
                     PMLDDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYII
                     EDDFISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLW
                     AKYSSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKV
                     RKSCGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSM
                     EELEKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            33353..34147
                     /locus_tag="JD016_02155"
     CDS             33353..34147
                     /locus_tag="JD016_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HAU4998432.1"
                     /translation="MFYDYHMHSSFSTDGKSTLEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            34189..35520
                     /locus_tag="JD016_02160"
     CDS             34189..35520
                     /locus_tag="JD016_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAU4998433.1"
                     /translation="MIFLKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLG
                     IYHMIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALII
                     SIVASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQ
                     IIEQIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYI
                     ISIKDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGI
                     VSGMVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSS
                     IFFYFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTI
                     TGMLIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(35621..36838)
                     /gene="cdeC"
                     /locus_tag="JD016_02165"
     CDS             complement(35621..36838)
                     /gene="cdeC"
                     /locus_tag="JD016_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HAU4998434.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDECNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(37021..37201)
                     /gene="ssrS"
                     /locus_tag="JD016_02170"
     ncRNA           complement(37021..37201)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JD016_02170"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(37316..38227)
                     /locus_tag="JD016_02175"
     CDS             complement(37316..38227)
                     /locus_tag="JD016_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998435.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESIL
                     DLVRGIKKLNNIDIVDIEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDDN
                     EELDYEDEELDCEDKELDYDYDELTDEGEIEANEDELVEDKNTGYENIENNEDIVYDE
                     ELYNINEQESEHNYNEKNTKTRAKKKR"
BASE COUNT        12424 a         6741 c         3908 g        15224 t
ORIGIN      
        1 aatatactat ctaactttga cacttcattt gaatctacat aatatatttt ttctccatct
       61 acacttataa aaatatatat tatatttttt tcaagtatat taccttgtcc tctaacagtt
      121 tttaaaataa cttgtccttt tttgtttact tcatctataa atttttctat atcttttaat
      181 tttaatattt ctattttttc ttctacgtgt tctgtgtcat ctgaatcgac caaatcaact
      241 aatcttgata agagacttgt aaatccaaag tatctaaatt tatctaaaag ttcatcttta
      301 tcataatcgc caaaaatcat actatctaaa tccacttcta ttggcacatc tcttataatt
      361 gttgcaagtc ttttactttg aatagctatt tctttgtttt cctctatttt cttttttaca
      421 ctacctttaa gcttatctgt attctctatt agattctcta tactagaaaa ctcttttata
      481 agctttatac ctgttttttc acctattcca ggtactccag gtatattatc agacttgtct
      541 cccataagac cctttaaatc gataaattgt gttggagtca tctcatattt ttcaattaca
      601 gaattaaagt tgtattcttc tacttctcca accccttttt tcgttataag ggtagttgta
      661 ttatctgaag caagttgaat agcatcttta tctccagtta ctatgtatac cttatatcca
      721 ttttcttctg cttttttaga aacagtacct ataatatcat ctgcctcata gccatctatt
      781 tccaatctat ttattctaaa tttatctaaa aggtctttta atggctgtaa ttgttctgcc
      841 agttcatctg gcattccctt tcttccagcc ttatactctt tatattctaa atgtctaaat
      901 gtaggtgctt ttctgtcaaa tgctacactt atatgtgttg gtttatatat atctatcatt
      961 ttaaatagca ttgttgtaaa tccatatatt gcattggtct ttaacccttc tttattattc
     1021 atttctggaa gagcataaaa agctctattt attattgaat ttccatctat tataattaat
     1081 tttttttcca aattatatca ctcctaaaaa ttatctattt ttgtaacata tatattatat
     1141 accaatatac tgttatttaa tattcccata ataataatag tatattaaaa ttaaatatgt
     1201 tgcaaacaat atattttttt gctataataa tatacatgac atgcccgagt ggtggaattg
     1261 gtagacgcag tggactcaaa atccaccgga ctaatactcc gtgccggttc gagtccggcc
     1321 tcgggcacca ttaaaaactc ctaaagctat ttattcaata gattaggagt ttttttattt
     1381 ttgatttaaa ttttaagtta ttttttgaaa ttactgtgtc tttttaaaat ttatataatt
     1441 atataaattt atcttctagc attaatttaa aaactaattt atttttatat aattttctat
     1501 tttatatcat aacttattac aactttttat ctaatttatt gtatacaaca acactacaaa
     1561 taaattgaaa taaaataagc aatccaagaa caactaaaac ccacttgctt attaccatta
     1621 cagaaaatac actcaatata gaaacaataa atataagaat tacaagaaca taggacatga
     1681 tttgattagt gatacgtcca gctttatcac gtaacataat tcttctttca tcattcatct
     1741 ctatcctttg attttttatc ttttcttcat aatctactgc tctttcaggt ttactccaat
     1801 acatatattg aagaatcata catacacctg gacctaaagc agctccagat aatccccaaa
     1861 taaaagcctc aaatgaaaac tcagtaaatg tagccaataa aacaagtaaa attccacaaa
     1921 taaaatatat aattccaaca tataacatat tctttttcat ttttactcct cctcataaat
     1981 aaatatttct tcaatactca tatcaaagta tctagctatc ttaaaggcta agatgataga
     2041 aggattatac ctaccatttt ctaatgaacc tatagtttgt cttgatactt gcaaaataga
     2101 agcaagttct tcttgtttta tgcctctttc ttttcttatt tcttctaatc tatttttcac
     2161 atctatcccc ctttaatatc tcaactacgg aaagtctact ttccataatt atacaactat
     2221 attccattta tgtaaagctt actttccata tttacaaaaa aataacagac tattttcagc
     2281 ctgtaatttt agaatttcat attgtttatt aattttaatg ttatttatta attccatatt
     2341 atttattaat tttaacttgg tttctgaatt ggaatccaaa cttctgtagt ataatcagga
     2401 gcattgatat cgccattggg ataaagttca atatctggta aattagcata cacataccca
     2461 gaacttggta accactcagt aataattctt ttttgcaatt cctgtattgc cagagcattt
     2521 ggcattggag aaacacattc aaatactgcc catgtgcctg atggtattaa gtattcatga
     2581 gtatcttcta atacaggctt atctgttgga gcagcaatat aataatctaa acccttacta
     2641 tttgtacaca cactaactcc aagcaatcca caaggttcgt tatttattaa ctctgaaagt
     2701 ttatcaattg ttccattttt tatgcattta gcccataact taggaacttt ctcaaaacac
     2761 tcttctacaa tcatgttcat actttctttt ataccaacaa tacgaaaccc tttcttttct
     2821 acaatacgat attgcatttc tatatttcct tttacataaa tgcttaatgt catcttagga
     2881 tatgctttta aaacaacacc ctttgctcta gctgcagatg gagaaatgtt atgtatattt
     2941 tgaaaagctc tattaaaaga tgttggtgaa tcgtaaccat acttctcaga tagttctaaa
     3001 attttgatgt tactgttctg aagttcaaat gcagccttag tcatacgtct tcgtctaata
     3061 tattcagata atggtacttc tgcaatatat gaaaacattc tttgaaaatg aaaggttgaa
     3121 caacatgcaa tttttgctgc ttctgcataa tccactccac ttttgagatt ttcttcaata
     3181 taagtaatag cttgatttaa tttatttatc cattccattt gatattacct ccaagcaaat
     3241 tatacagttt aataaaataa tatacctctc tattcatgca caaaattgta agcatttaaa
     3301 tttataagca tttttcaatc aatacaataa atataaaaat tgtattatac aataaaaaag
     3361 ttcaataaca ttatattata aaatgttatt gaacttttta taaaaattaa tttaagtgta
     3421 gttaaattaa gtattacact atcttatata ttacaatatc ctactatctt atagtcttct
     3481 ggcactttat aatctttgtc tggagtacct aaattccatt ttaaatcaaa catagttgca
     3541 cttattattg ttgcaaaata aagcataact ataccaatgt ctattttttc ttcatataaa
     3601 ttagtatgac catcttttct aacagctaaa actacagtgc ctccatcaac tataaatctc
     3661 catggttgtc tatttaatgt agatggagct aatcttgcat agtgaaatgc atcaagaagt
     3721 gctctttctt ctaagatttc tatacttgca acatttcccc attcgttcat ataaacaaca
     3781 tcttctacac ctagtctagt agacccctta tctgttacaa cttgcatatc agattttgaa
     3841 taattttgcc cagtttttgt agcatttaat actttagcat tacttacatt ttctccatat
     3901 ccaatagcta taattcctgt tacctcttta tctgaattaa ttccaagttt ttcttttacc
     3961 ttttttccat cttcaaaagt aacccaacaa gaaccaattc caagttctgt tactttaaag
     4021 attatacttt ctcctatgta ccctgaattt tcaatgtaat tatcttttat gtcagataat
     4081 attataatat aacttggaga atctatcata tttcctttgt accctgctac tttatctaaa
     4141 ttattaaaaa cttcacttct attcataatt cttacatcaa catctatttc tggaactagt
     4201 ttcttacaag attctgagta tttcttaatt tcactaagat attcttcttt aatatctgtg
     4261 ttcttatatt ctctaacaga ctttctattt aatatgattg ttttataatc cataatttta
     4321 tcctccccaa actagtattg tcttaattat atacccatta atatattatt ttactcattt
     4381 atttaataaa taaagattac aattttgtaa ttattattta ttaataatat tccactttta
     4441 cattaataat gaccagttgt gcaaaatcca ttgccaacca ccaaataaaa acatattata
     4501 aaggtaatat aaaataacaa acaaatctta acaaaaaatt aaactttttt tacacgaaag
     4561 aaggcttccc gttgccttct ttatttttat aaaaaaataa gctgtttcta aaatgatttc
     4621 acatttttga aacagccaag ttattctata atgacaacta taatttgagg atgtgaggga
     4681 gagttgtcat tataaattta gggtaattat attatgtttt aagctagttc tttctatatt
     4741 tatataatac attttatata tgaattttat atgagattgg tataaatgat atatgaaatt
     4801 acatatgttt atattatatt aaattttatt attacatttt ccacaacaat actcatatgt
     4861 ctccacatta tttggaatat ttacaaatac aaattctttg cagttctcac aatactttaa
     4921 tatagatata gttccacaag tgtaacattc aaatattaca caatcctcat tttcctctac
     4981 tatttcaaac tttttactat gttcaccaca ctctgggcac ttgtgattat ttacattcat
     5041 aaatactcct ccttattagt atagttacat gtcaaaaact taattaatgt attctatatt
     5101 acttattata tattctataa taaatcatat tctaatataa taaaacaaat taaataaaaa
     5161 aaaaccataa attttaaatt tatggattaa ttcttgtaat gataactcta tttgaggatg
     5221 tgagggaggg ttatcattac aatagggtat tatattatgg tttttaagct agtctttcta
     5281 cacctcatat gatacatatg ctatatgaat tttatatgat ttttacataa atattttatg
     5341 aaatttaaat tttttagtca ttttgaaatt tgtaaaaagt tctctaaaaa tccacagcat
     5401 cataaacagc caaaatttaa gtagaacatc ataaaaaaat ttgtccaata attttaacaa
     5461 gtaatctcac ctcatcataa ttattgacac aaacttaaat aaacaatcta taaatcttct
     5521 ataaatctta aactttcccc ctattgatac acaatttcta gtttgtcaat tttattttgc
     5581 atttctaaga aaatttcttc aacagaattg ttaaatatat tgcctaaata gattctttta
     5641 tctttttgaa atgaataaga ttttacacta ccatcagaag aaatatataa atgactccac
     5701 tctgctccat attttgaaaa cctctccttt gtatctttaa acctcatatc ttctattttt
     5761 attgcttttt ctctgtctcc tcttactaca gtgtgacccc tttttattaa actggttatt
     5821 tttatatctt cttttaattt aatacgtgta tcatcatttt ttgaatatac tttccatatc
     5881 actttatgac gataattatc taattccttt attaagtttc tatcaatagt aaaactatct
     5941 ttagttacca caattaaatc aaaatggtct aacatatatt gaaatatttc tctcatactt
     6001 ttatttttta aaatatcctc accaataacc tctataaatc tacagccttt ctcatacatg
     6061 cagttcaata aacttgtgat ttcagaaaaa taatcatttg tattattatc tccatctatt
     6121 tctatagtaa agtacaaagg agtaatgtat gatttatttc ctgttatctc aaatacactt
     6181 gatagtgtct gtatatcatc ttcaaacgtt aagtatacat tctcataaaa cataaaaaat
     6241 tccataactt tttctttcat attatggata tttctatatc tattggataa tatatttatt
     6301 atatcgtcag tagtattttt cccatcacat tcagccagta taacaatttg cttccaactt
     6361 aagctatcca catttccgat acttttttta tatccatctt cttcatcata aataggacca
     6421 attataatgt tttcttttgg ccacagatga accctagttc catcttttaa tttcggatat
     6481 ttatttttta cagactcata aaatctactt gtatcaccca tgattcttta ctacccccca
     6541 tagtttaaat attctatgct aaaaatagca ctttattttt attaattcaa caattattca
     6601 aaattggtca ttatatatgt ttaaatggat ttaacatcaa ccttatttat atgttattga
     6661 tgtttatata tgttatataa aatcttattt ctcatacttc aatgtaatct taactatctc
     6721 tggtttacaa ttaagcctaa ttaaaacttt tgagcctcct aagcctttat ttaaaatcat
     6781 agtgctatta tctttttcat attttcccca gtcatactta ggaaagaact ctctatttgg
     6841 agataataca cctccaacaa aaggtattct tattatacct ccatgaacat gtcctgctaa
     6901 aaccaaatct gctccccatt ttgcatattt ttcaaagaaa aatggtgtat gtgctaaaag
     6961 aatgttatat tcattttcat taactttacc tagattgtct tctacaaaat tgtcacttag
     7021 ttctaatttt ttgttattag caaataaata tggatagcat tccaatggta taattaaacc
     7081 atataaatta agatggctat tacctctata tattcttaca gattcatttt ccatatttat
     7141 tattttcttt gaatatagtt ccttaaaata atccttatat agatgtttat acttttttat
     7201 taaagatttt tgttcatgat ttccaattat atgatatact tcatattttt caaccaaatt
     7261 atctattaaa tctaatgcaa cttgaaaatt tttattttct ccatctacta aatctcctgt
     7321 tataaatata acatctgggt ttaatgattc aattttatct atcaatacct tattattttt
     7381 atcaaacacc ttattatgta aatcactcac ttgaactatt ttaaagttat caaattcctt
     7441 tggaattttt ttgttgaaaa tagtatattc tgtcgtttct atatgctgaa tttcatactt
     7501 atacagaata tacaataata ttaatactaa tactgtcatt gaaaccttta taatcatatt
     7561 ctcttcctct ttattctatt ttgttaatat tattataatg cttttatgtt ttttttaaaa
     7621 caataacatc atcacataca tataaatctt ttcgacatta taaaaataag ttatcctttt
     7681 ctaaaatttt catttatttt agaaaaggat aactataaaa ttatctataa acctctatat
     7741 tgtgaaatat agtttttcct ttatttaaat ttttatatat atgctctaaa gttccatcaa
     7801 acctaataga atgtccagac tctaaaatat aagttttccc atccacatta aggtcaagtt
     7861 taccttctat tacgagcaca tattcttcta aaacatgact atgtgtagat gaaacatgag
     7921 tacagttttc ctcaagctca attataaaaa gttcaaacct tctttgaggg ctaaaagggt
     7981 atattggata taacttcatg ttcccttttt gctcaacaat aggctctact tcttcttttt
     8041 ttataacctc ataggtattt gtattttcat ttaatagtgt agtaaaattt attttaaatc
     8101 cagatgaaat tttccataaa gtagatactg tcggacttga ttcacctctc tcaatctgtc
     8161 caagcattgc tttactgaca cctgtcatct ttgaagcttt gtctaaactt atgcccattt
     8221 cttgtctcaa taaccttaaa ttttctccca cattaggaaa tttattatcc atgttaaaat
     8281 cctcctgata gctatttgta ttctatgatt tttactcctg taagttctac tatctctttt
     8341 aattttaaca tatgattgtt acaaatttca tcaaattcat atatataatc ttcaagccct
     8401 aatgccttat atttatcgat tccaaattta tgatatggaa gtaactccat cttgcctcct
     8461 ggaacatttt gtttgacaaa taatgcagta tttcttatat tttcttcact atcatttact
     8521 ccataaataa ttggcactct tataaccata gatttattca actttgaaag cctacatata
     8581 ttatctaata taattttatt acttactcct gtatattctt tatgaatatt atcatccata
     8641 gattttatat ctacaaatat gtggtctatt ttctcaaata catcatttac cttattccaa
     8701 tcaaaatatc cacaagtttc aattgcaata tttatgcctt tatcataaaa aatatcaact
     8761 aattctctta aaaaatcaat ctgaagtgta ggttcacctc cagaaaaagt tatccctcca
     8821 ttagattcaa agaaaaatat aaaatctttt tcaacttcct ctactatttc ttcaacactc
     8881 atttcttcgg tcattataca aatggcatct ttaagacata ccttgataca ctctccacat
     8941 aaatcacatt tattatttat ttgcgttcta tcaaatagtg aacttatatt ttgaggacaa
     9001 acatctatac atctattaca ggatacacat ttatctttca taacacctaa ttttactatg
     9061 ttactccagg aatctggatt agaacaccac ttacatttta atttacatcc ttctaaaaat
     9121 atagtagtcc ttattccatc tccatcattt actgaaaaat gttgtacttt tacaactcta
     9181 ccttttttac tcataatcta cctcataatt aaaaattgct atgtgaattt ctagctatta
     9241 tagcatcttg catttctctt gataaattaa caaattgagt actatatcca gcaaccctca
     9301 ctaataaatc tttatattct tctggatttt cttgagcttt taatagagta tcatttgata
     9361 tcgtattaaa ttgtacatgg aatcctccta aagcaaaata tgatttaatc atagatgtta
     9421 gatttctaag tcctctttca gtttccacca aatcttgatt cagtcttaaa tttagaagtg
     9481 ttccaccagt atgaacatca tgatttattt ttgatgcaga tttcattgct gctagtggac
     9541 ttgttgtatc agtaccaaca aatggagaaa ctccctctgt caaaggtttt gtcgcttttc
     9601 ttccacaagg agttgcacct actatttttc cagttggtac atagtttgat atacccataa
     9661 atgcactatt aaatttagag ccaaaaatat ccttgtattt tctagtttca gtataataaa
     9721 aatcagaaac ttcaattgct aatgaatcta cataatcatt atcatttcca tacttaggaa
     9781 catccaacgc aagttttctt aattcttcat aaccttccca atcattattt agtgcttttg
     9841 ttaattcttc taacgtagtc accttatcct caaatactaa tttttttata gctgccaatg
     9901 aatttgaaac tacacctaac cctataccag ttaatacagg acctatatta tattttgctc
     9961 ctcctttact taaatcaact cccttatcca aacatccatc tacacaagta gataaaaatg
    10021 gtcttggtac catttcttta tgaatttgtt gagcaacagt agttcctata acagaacttt
    10081 ttacaagata tgctaattgc tttaaaaact catccttaag ttcttcaaaa gaagtaaatt
    10141 ctgtgatatt ttttgtatct aaccccattt tttctccagt taatctactc ttaccttcat
    10201 tcatagcata ttctaaagct gctgcaaaat tgatatttac ggctgaagtc cactgtcctg
    10261 tttttctaaa atgaggaact acacaaccac agttactcca atctctagca tcttctgcat
    10321 catatccatc ttgtagtaac atttgtgccc ctgcactatc gctgtgtata gcagggaaac
    10381 cagttccttg ttttaccaat ttagcaactg ccattacgaa attgtctggt gctccttgac
    10441 tcactctaac acttaatcct ggctggtgag tttttacact ttctgtagcc tttaaacaca
    10501 tatatgagat atcattcgtt ccatcagtac catttctctt tttaccacca acagtaagat
    10561 tttggaattg attatatcct gcaaagtaat ttgctgtatt tgctgatata gtccataccc
    10621 attctgataa cttaatccaa agtgcttcta tcaattcttg agcttctgat tcagttattt
    10681 taccttctct agcatcattt tcataatatg gatacatata ttggtcaaat ctacctaaat
    10741 ttaatgccaa tggattttct gatagaatac cacctaattg aacaaaccat acaaattgta
    10801 ttgcttcata aaaattagtt ggagggtttg ctggtacatt catacatatc tctgaaattt
    10861 ttataagttc taactttctc ttttcatctg tttcttgctt actcatctct tttgcaagat
    10921 ttgaatatct ttgtgccaat gtcataatac cctgtgcaac tatagaaaca gatttgtaaa
    10981 aatctatctt ttcaatattt tctgaaactg atatatctaa ttctttaatt tttttatctg
    11041 catcttcctt aatcccttta tatcctttta caaacaatat atcttgataa tctggtgtta
    11101 cttcaccaac tgcctgtctc cacttagaat cattatctat tattcctgta tctactaata
    11161 tcttagctgt atcttctggg attcttgcta agaaaacttc ttccaaagat tttcccttcc
    11221 aatatggaaa tatttctttt cttataaatt ctatttgttc ttttgatatt acatatgggt
    11281 cttgagttct tttatcaaaa gtatccattt ctttgtctac ccattgccaa gaaaactctg
    11341 gtgtcaatat ccctgttctt ctaaattttc ctgctgtacc tactattaat tcattttcaa
    11401 agatttttat aggaagttct ttacatgtct ccaaaaaagc tttagctctt cttatacaga
    11461 tagcttctcc ctcagttttt ttatgtgatt ctgtaaaaat tctagccctt tcacaacata
    11521 tcataggttt agaattaata tattcttgtc taaatgcttc tattctatta gtttgactca
    11581 tttaaaatcc ccctttggat aatatatact acatttttgt atgttataac atactttaat
    11641 tatattatac tgtcctttag cataaaatgg aagcaaaata acttattttt tttcatctac
    11701 cttgaacacc tatatattaa tacttttttt gttaaattta taaaatctaa aaaaattttc
    11761 tatgatatat tttctatatt gttatagtta tttctaatat actaatgatt tgaaatatca
    11821 aatttgtata ctagacttag ttttaaaacc ttagttgatt gtttaacttt gttaacttca
    11881 acatgtatat ccttcatata aggaagctct acaacaccta ctcccttatt aaagtcttct
    11941 tcaaccatag aaaaaatttc tttctcagtt aagttcgtat tagtcaatga tttaaataaa
    12001 ttgtatataa atttaatata tttatcactc tcagataact tatcatcttc tggatactct
    12061 ttagcacaga gaatatctat tgttactttt ccttctttat cgtcttcaga catagaatag
    12121 aataaacttc gaatattttt ttcaggtggt ttttcagcac catttataga tactacaaag
    12181 ctaccatctg attttgagtt agtaatacta taaccactgt ctattatagc tttcgaaaat
    12241 ctttgagata aatctttttt tacttcttct ctacctttcg catcattaat cttttgtgag
    12301 taattattaa atagctcgat agattcatct tttttatctt caacttgttc tttcaatgaa
    12361 tctttactaa tttgttctga gctacttaag ttcttatcag atacaggaga catacaccct
    12421 accatcaatg ctaagctaag atttaaagcc acaatgattg ccactctatt tatattcctt
    12481 tttactttca tacacaatcc tcccatcaca ctaaccgctc taaagtatta ttcattgcac
    12541 tagaatatat ttatattttt tttatattaa agatttatta ttcaatacat atcaccttcc
    12601 tatccaattc ttataactta ctattagtaa aattataagc tccttaatac agaaataagg
    12661 atgttatctt ttgttattaa aagataacat ccttattttt ttgtatcaat acatatgtcc
    12721 caactggtaa cttaatcgcc ccaactgtca gtatcttggt ccaatggtat atatatattt
    12781 agtataaatt tatcgatact attttcaaat aataattctc cattatattt ttgtaaagaa
    12841 gatttaatac tctgaatacc tattccatgt acaaacttat ccattttgtt tgttaacaat
    12901 atatttttat taaaagtaag ctgatttagt ttactatttt cacatttaag tacaaaaaat
    12961 gaccttgtca tagttccttt aattctaata tatttattaa cattttcatc cactatttta
    13021 tcacatgctt ctattgcatt atccaaaata ttagaaaata tgcttgacac atctataggt
    13081 tttacaaaat ttacttttga aaaatttata gagcaagtaa atttaattcc tttttttaaa
    13141 cacatttcac tcttttcatt gattataatg tctaatatca tatttcctgt attatagata
    13201 tatttaaatg tttttatttc atcttttaaa ttgttaacat attcattaat ttctttgcta
    13261 ttgtttctaa ggttgtctat acaataaatg tgattattta tatcgtgata tagtttctta
    13321 actcgcatat gagattcttg aatacttaaa taatgcgcat attgcatatc taacttattt
    13381 tttattaatt cagcttgaga ttttaatcta ttctctttta ttatttcttt aaacaataat
    13441 ataagaaata tactactagc tcctaccaat tttggattaa caaaattatc aaaatagtat
    13501 ccttctgaat gtaatgatcc aaaccgttct aattttttta atacataatt tacaagcata
    13561 ttcattgtat taactaataa ggcaaataat aaatatacat aatttctctt atcttggcta
    13621 attaatttta tagactttct aaaaaagcta aatataagat atataagaat aattattaaa
    13681 ttactatata tctgaatttt tatagattct aagtgaggta ctaataaaga ttctttagat
    13741 gaaataacat tacctgtaat tgcaaaaaat agtattctat atataacagc atccaaacat
    13801 ttataatata cactaaatag ataaaagaaa atggaatact taatataacc catcttataa
    13861 aaataattat agtatcctcc ccaagcaata aatgctaata tataaaataa atagtttata
    13921 ttactaaagt aatttcctcc attaaataat ctacttatct gaattataat ataaataaga
    13981 aacaagacaa tatagaatac tattttttca ttttttttac tatccttttt ataaattaaa
    14041 attccgttca ttatagaaaa ctgtataaat atagataaaa ctactaatag ctcagtcata
    14101 aagtttagca tattgtatca ccaagaaatt ttaaaaattt ctccttaact tctttatatc
    14161 tatatctact aataggtacc tcttctccac tttccagtat tgcagtatta ggctttatat
    14221 tttcaacata tcttaaattt actataaaac ttttatggca tcttataaat ttatatggat
    14281 ttaattcttt ttctattttg cttaaactat attttatatc aaaattcttg tttattgtgt
    14341 gtattagcat atcttttttt tgaacttcta tatattttat ttcatttaaa taaatcttat
    14401 atgtattaga cttatttttt attgtaatat gactttcctt atttatttca atatctttga
    14461 tacaagttag cacatgtttt tttagttctt ccaactctat tggctttagt aaatacctat
    14521 aagctcttac ctcataacct tcttgcacat aatctattaa tgaagttaca aatattattt
    14581 ccatttcatt gtctacttct ctaatctttc ttgcagtatc cattccattt agtttttcca
    14641 tttttatatc aagaagtaat atatctatat cttttaaatt actttccagt aactcttctc
    14701 cagatatata cttttgtatc tcatattcta tacttatatc ttttaatata ttctcaaggt
    14761 aatcttctat taaaagttgt gtttcttttt catcttcaca aactgctatc ttaatcataa
    14821 aatgcctcct tacataattt ctagttaata caattaaaat atttataata tatttaaaga
    14881 taattttgta tatctgatac tttaggataa catttttaaa ttatttttat caatactcaa
    14941 tcccatacaa ctattatttg atatttcaat ttaaagacag aaattactat aattaaaaac
    15001 agtactttat taacaaaaga agatgtctta aaatattaaa aaaataattt taggacacct
    15061 tcttttattt atttaaactt tttaagcctt aaataataca ttaatctcct atataattgt
    15121 actgcatata aaatcaacaa tataatctgt agtacaatag cagttacaga tgctccataa
    15181 ccactatata aatttcttat ttcaatattc aataaatata taaatattac tactaacata
    15241 aagaatataa acatattcat aaataaaatt gactttactt tgtttttttt atcagaatca
    15301 ctatataatt taggaggtat gtctttgtat tctttcgacc atatatacca tgcagatgta
    15361 cccataaaac taccaatact cctaaataca agattccagc catttgattt atgttcatca
    15421 aaataattat tctctacatc atagtagtaa tcaacagtgt attttatttt tcttggactt
    15481 cccttatgaa aataaaatat tcctccaaat ttatttatct tatataaatt atatccttta
    15541 gcttcaatat cttctaacca tttctctatc ttatcaggtg aactatacca gtcaaatttg
    15601 aattttattt tcttttccat agcactcatt ctccaaaatg atatttttaa atagcttaaa
    15661 aatctattta aataattata tcattttgat actaatttta ggttacaatc atatatttaa
    15721 aagctattta aatagtaata aactaatagc cataacaccc attcctgcaa tcaatccata
    15781 aatagataaa tgatgttccc cgtactctct agctgatggc aataattcat ctagtgatat
    15841 gaataccata attcctccta caattgcaaa tacaattcca agagttatat catttaaaaa
    15901 atttctaaga agtacatatc caataatcgc tcctaaaggc tcagacatac cagataaaaa
    15961 ggaataataa aaagctttct ttttatctcc tgtggcataa taaactggta cagatacaga
    16021 tataccttct ggtatattgt gtattgcaat tgcaattgct atcggaatag ctattgtaac
    16081 atcatcaaga gctgatataa atgtagctaa tccttcagga aaattgtgaa ttgctatagc
    16141 cattgctgta aatatacctg ttctcaaaag agacttattt tttttgatat tcttttcttc
    16201 tatattctcc attttcttta tttcatgtgg attttcttct tgtggaatta gcttatcaat
    16261 aacagcaatg atagccatac ctataaaaaa agctattaca gttgaccaac ttcctaattt
    16321 ctctcccata gattttgtca aagcatcttg tgctttagga aaaatttcta tcatagaaac
    16381 ataaatcata acaccagcag aaaatcctaa gcttatagaa agaaacttag tatttgtttt
    16441 tttagcaaaa aatgctatac aacttcctat accagtagat aaaccagcca ataatgttat
    16501 caaaaatgca aatattacat tattcaaata aatcaccttc tttttaattg atattgatta
    16561 tcattttctt tatgctatca tatatatttt atatagtcaa tatttataaa aaatatacac
    16621 aataaaaaag cagagatata aacttagtct ctgctttttc tttatagtat attaaaatta
    16681 tttatttaag aaatccactg catattgcac atataaagca gttccaattt ctagagcatc
    16741 ttcatctata ttaaagcatc catgatgatg aggataacaa gcaccttttg actcatttct
    16801 agctcctaca aatgctagtg caccaggtgc tatattcata aactcagcta aatcttctgc
    16861 tcctgttact ttttcaaata gtgttataca gtcttctcca aatagcttaa ttgctgcatc
    16921 tgtagctatc tttgaacatt ctttgtcatt tataactgct ggtgttaagt acccatactc
    16981 taattcagca tctgctctat atgcttctgc tgtagatttt gctatacgtt ctaaaattcc
    17041 aggaatttgc tttctaagct ctggattaaa tagccttatt gtaccttcta agatagcttc
    17101 actcgcaatt acattaaatc tagttccaga atttaaaact ccaacactca caactaatgg
    17161 ttccagagga cttacttctc tactaaccat agattgtaga ttcataacta ttgctgaact
    17221 tgcaagcact gcatcaacac cttgatgagg aagtgaacca tgaccacctc taccttttac
    17281 agtaatttta aagaaatcag cagaagccat tcttggtcct tcttctaccg atatggtacc
    17341 agattctaca tcagtccaaa gatgtattcc aaacacacta tctacacctt ccatagcacc
    17401 atcttgaatc atagctcttg cacctttacc aacttcttct cctggctgaa aaaataattt
    17461 tacagttcca ttgatactat ccttaatatc atttaacact tttgctgccc ctagaagcat
    17521 tgatgtgtga ccatcatgac cacaagcatg cataagtcct tcatttttag atttgtattc
    17581 tacatcagtg cattcaacaa cttgtagagc atccatatca cccctaagtg ctacagtctt
    17641 tcctggatta gctcctttta tagttgcaat tacacctgta cctccagctg atacatatgg
    17701 tatccctatt ttatcaagtt cttctttaac tctctttgat gttctaactt cttccatact
    17761 tttctcagga ttttcatgaa attcccttct aagcttaatt acatagtctt tataactctt
    17821 tgttatttct ttaatatcca ttccatacct ccaagtagta cacattcttc ttatttaaaa
    17881 tactttttat cacttaaata ctatattaga tttacaaaga tacccgctat tactacagaa
    17941 gctattgtaa ctgtagtaaa tcctccaaca agcatctttg gaagcatctt atccattaaa
    18001 aattcttttt cttctggtgt ttcagccaaa gcctttactg cctcttcagt taatatatag
    18061 tttggtggaa aaccataaag tgatgtaagc gaaactgaaa aagccatttc tttactttca
    18121 cctaaaagct tacctactat aatagagaat attcccatac ctattacacc aaatattatg
    18181 attcctacaa gaggaactgc catttcaaca agcattgctg gtgtagcttt tgctaaccct
    18241 gcaaatataa aagccatcaa tgatgtcatt aaccatccaa atgaaccaga tttattaagt
    18301 ggttttctat caactattcc aagttctgat gctattacac caaatattaa acatataaca
    18361 tatcttgata caacttcatt tgtaagattt gtaaattgta ctgctaacca tgctacaaat
    18421 cctaatctaa gtattatcat atatgtcgta tcatattttt ctggtaagtc tggtattatc
    18481 ttaaatctgc ttttttgttc aggtaaatcc ttaacttttt cttcatctgt atttacagtt
    18541 acttttccac ttctaaagtc tgataaaagt ctttttcctt cttttttaag cattaatgct
    18601 gtaataggat aacccgcaaa tccttgtgca acatacatca aaattgcaag tactgataaa
    18661 cttgcaagac cttttgctgt agcagcatct gccatcatta tagatgcaac tagccctcca
    18721 gtaagaggag gtgtagctat tactgctgta tcccatccaa atactactgt ccctaatgcc
    18781 aaagcaccta cacatattcc tactattcct gccaaggcta ttactattgt tttccattga
    18841 cctgctaatt ccttaatact catcatagtt cccatatgag ttattaacaa aagcatacct
    18901 aattgagcta atggtgttcc taaagcggct aagtccacaa tattttctgg gaaaaaagtc
    18961 caaaatccta ctataaatat aattgctgat acaaaaaccg acggaacaaa agctttagtt
    19021 tttgctgaaa ctatatctcc tatcgtcagt gatactaaaa ctagacaaaa agctgttatt
    19081 gcctccatac aaaatccccc ctcataataa atatattttt ataaaatgta tgtatagtta
    19141 tccattattt tactactttt gaaaaacatt tcaagtcaaa attcgatttt tttcataaaa
    19201 ttcgataaat tccataaatt aaagttagtt ttctaaatta ttctttttgt aaaaacgtta
    19261 tcccgaccat tggctattta actctattct tacaaagaga attaaatgta atttgcaaaa
    19321 aataaaacat aggatacatt ttattcataa aacatcttat aaatataaaa tgaattgtct
    19381 ttatgaatct taatatatcc tatgtttgta agtttaatgt gtatgttagc tatggtgtaa
    19441 aatttgtata ctagttattg tgtaacctac tatatactcc atttttgttt actagttctt
    19501 catgagtacc tctttcttca attcctttat ccgtcaatac tatgatttca tcagcatttt
    19561 ttatagtagt caatctatgt gcaacaacta aatttgttct atctttactt aattcctcta
    19621 aagactcttg aatttctctt tctgttacat tgtctaaagc agatgtagcc tcatccaata
    19681 ttattattgg aggattcttt aaaaagattc ttgaaattga aattctttgt ttttgcccac
    19741 ctgataattt tacgcctctt tctcctatat aagtatcata tccatctggc aaagtctcta
    19801 caaagtcatg tattctagct tttttagcag cagctatcat ttcttcatct gtagcattag
    19861 ggttcccaca aagaatatta tctcttatag ttcctgtaaa taaaaataca tcttgttgaa
    19921 ctatacctat attttttctc aatgatttta aactaacatc ttttatactc tttccatcaa
    19981 tttttatatc acccttatca aattcataaa atcttggcaa tagattacat aatgtagtct
    20041 ttcctcctcc agaaggtcca actaatgcta tagtcttacc agcttttata gataaactta
    20101 aattatccaa aacttgagtc ttatcttcat atgtgaaaga tatattttct atctcaatgt
    20161 caccttttac attttcaagt tcaataggat ttttagcttc tttttgatgg tcttgttcca
    20221 ttatctctat gaatctttta aaacctgtca taccattttg atattgctct gtaaagttta
    20281 ttaatttttt cataggttgt ataaacattt ttacatatag tatataagca gcaaaatcac
    20341 ccaaagtaat ctttcctata taagtaaaat aaccaccagc tattacaact acccactcta
    20401 agatatccat gtataaattc ataccagagt aatattctgc cattacctta taagaatctt
    20461 gtcttgctct tctaaataat ttattgcttc ttacaaactt atttaactct tctttctcag
    20521 tacaaaaaga cttagttacc ttcatacctg ctatactatt ttctaaagta gcatttacat
    20581 cacctatagt aactctactc ttcataaaag caatattcat cttatctttt ttagctattg
    20641 caaaccaaat tataaacggt aatatagcaa atattataag cgtaagagga atatttatat
    20701 ctataagtat aaagaatgaa ccaaccaaca ttactataga aatgaataaa tcttcaggcc
    20761 catgatgtgc aagttctgtt atatccatta aatcatttac tattctagac atagttactc
    20821 cagatttgtt attatcaaaa tacgtatttg gaagtctttg taagtgtgta aatacctcat
    20881 ttctcatatc agcttgcata tctacaccaa taacatgtcc ccaatactgc ataaaatagt
    20941 taagccctgc ttttgctata aatataagta taagtgctac tgcaaaaact actaacattc
    21001 ttaagttttt atttggaaca acgtcatcca ttatatttct agttatcata ggataaacta
    21061 aatcacataa ggaaaataga aaagctgcta taaggtctag tgtaaaaatt ttcttatatg
    21121 gcttataata tttgacaaat tgttttatca taagtattaa atccctcctt tattagtact
    21181 tctaaaaaat acccataaat tagtataaca taacaataac tttaaatctt ttataaactt
    21241 acaaagtaat aacaatcttt ttcataaaat tgaagattta tatcatatag ttgtatactt
    21301 gaaacaacat aaaaagagct atatctctat atttaaaatt ttataaagat acaactctat
    21361 atttaatact aaattttttt tataaataca aaactacttc aatgcattta atatcttttt
    21421 atctaatgct gttgtattta tacctgttat atatcctgtt gcctttattg taggtatatc
    21481 ataagttgtt ggtagtatag tagtagatac gattaaatca gcaccttctt gttttgttga
    21541 acattcagca attttacatt gctcaacttc tgcttgtata ttattttctt taaccaatct
    21601 ttctactctg tcacaaacta ttgttgatgt tgcgattcct gctccacagg ctactaatat
    21661 ttttttcata tattgtcctc ctacaatttc tttaatattt tataactcaa aataaaattg
    21721 ataaatttct tatatcttta tttatataaa taattatatc attttatata agaagaacat
    21781 cttaaaatta ttcttttaat atgtttttta tttcaacatt gcttatactt aaaatttcat
    21841 tttttataca agactctatg tactttgata tatttttata atcatttaaa ttaaactgtt
    21901 caatattcaa tttatattct atatcactga caatacctat caaatcagac ttattagata
    21961 aaatatcctc atacttacct tttctagtta cttccaatct tttaaatcta taatttttat
    22021 atccttctat gataatcaag tccatatctt catataattg aatcatttta tataatggat
    22081 tcttctcttt tagttcttca atatatgcta ctcgtttatc tgatattata cagacttttc
    22141 tcgccccaga ttttctatgt ttataagtat cttttccttc ctcatccata tcaaaatcat
    22201 gacaggtatg tttaataact ccaacacgat accctttttt atttaattct tttattatgc
    22261 cttcaatcaa cgtagtttta cctactcctg aatatgagac aactgacaat atattataca
    22321 taaaattgtg tcctttcata aaactatcta ataacaacat ccactaattg attttctttt
    22381 attgtctcat tctttttaat cttaatcata caattacttc ttagagttgc agataatcta
    22441 gcttttgcat ctttcttatc atttagctct acatacaatt taccttcact tgtatatagt
    22501 tttccactta caaatctatc tttatttgaa agtttattaa attctccttt aaatatagct
    22561 tttatatgat gtgttccctt cttatatgtt aaatactcaa taacaggaac tatcacatta
    22621 tcaaatacca ctattgctgc atgtggatta ccagaaagac caaataatag cttttctccc
    22681 aatgtaccta cataacaagc tccaccaggt ttaatgttta tcttccaaaa taattctgtt
    22741 gcccctattt ttttgaatac atctggaatt aaatctttat ctccaacaga aacaccacct
    22801 gtggtaatta aaaaatctac actctgtaac atattcttta cctttttttc taactccaat
    22861 atatcatcag aacaattgca tattgtatct gcttgtattc caagttttaa taatcttgaa
    22921 tataaggtat atctattact atcgtatatt ttaccattct ctaaagtctc atttatatct
    22981 tgtagttctg tacctgtatt caaaattcca acttttaaat catcataaac agttactttg
    23041 gatattccaa gacttgctaa tactccaata ccatcatatc ctatattctg atttttatta
    23101 ataatcaatt gccccttttt tatatcctca cccttaaata tgtaattatc gtattccttt
    23161 aattttatat ttaaagttat catatcacct ataactgtta catcttcttt ttttattaca
    23221 caatttgcac cctgtggtat ctttccacca gtcattatct tcactgcttc atttttattt
    23281 aataccctat taaatacatc tcctgcatac aaagtcccta taacctttaa atttctattt
    23341 tcagaatttg cattatatgc ataaccatca tagggtgacc tatcaaatgg aggactatca
    23401 attgttgaat atatatcttc tgcaactacc ttatttactg catctataag atttactatc
    23461 ttgaccctgt ttattggtaa aacatttttt tcaataattt ttatggcatc ttctaattct
    23521 atcattttaa atactcccca atatattttt tatttaagat atttctatgt aatatattat
    23581 agattataaa agttggaaat acttttatct tatatttaat tataactaat agttatctat
    23641 aagtgcagtt caaattttat aaatctaata aaaaaactgt tcccagctag aaatttcctt
    23701 ctatcttgaa acagcatcat atgctttaga atattttatt ctaataaagt tccttttatt
    23761 atttttacaa ttttatcact ttcaatagtt tttatagatt ttattttgtt atccacaata
    23821 cttattgtaa gtacctcttt ttttaatatt ttgttatctt ctttcaatct ataactctta
    23881 actactttac tgccttcatg attatatgct gttaaatcat aattcgcatc tcctacaatt
    23941 tcatttattc tggaatcttc aacaccaaca tactctccca ggtcaatatg tctaaaattt
    24001 tctagtgata cagccgattt ttcatggtca cttttttcaa tcactatact agattcagca
    24061 tcaatgactg atataccttg tgatgagaaa gaattggttt caacattgac aactttattt
    24121 tcgttaatat caacatataa agcacttcca tctagttcat catttccagt atattttgga
    24181 taaactaatg tcatataact tgatgtattt atatcttgca ttgtatttga ttcttttaaa
    24241 actttaggtt ttatataata aaatgtatta taaggtatac ccatactctt tataatgtac
    24301 ttataatctt tattcataat ttcattaaca tcattttgaa cttttgtcat agttttttgc
    24361 tgtgttgctt tacttgctac ttgatttttc tctctagtat tttctttatg acatccaaca
    24421 agtataatac ctaaaatagt tacacaaact aatgaccttt ttaacttcat aaatcaatcc
    24481 tcctttctta aattttatag tatttatcta tatctcaatt tacagttatc tcttttttca
    24541 ataattatat ttattatatt ataaaataat tatataatat ttatttgaat ttaataagat
    24601 taagaagcta attgtaattt ttctgtaata aaaaacactt taaagttgaa ttttgaaaat
    24661 tcaatttacg atatttttat tgtaagtacc ttacaacatt ataccaaatt attcctaaac
    24721 aatcaaaaac tccatgaata tttaaaggtt ttctatttta aaaatcacct ttgactatta
    24781 atttatcaaa tatgttataa tagttttaga taatttaagg aggattaaat aatggatttt
    24841 aaacaacttg aagtatttgt tgctgttgca aaacatcaga gtttttcaaa ggctgcaaga
    24901 gaactttttt taactcaacc tactgtaagc gcacacatac agaatttaga gagagagcta
    24961 gaaactgttc ttataaatag aagcaacaaa gttattacac tcacaaaatc tggtgaaatt
    25021 ctttatgaac atgctatata tatattgaat aattgcaaac gtgcaattta tgatataaaa
    25081 gaatattcag gaaaaataga aggtatcata gatatagcat gtagttccat acctgaaact
    25141 tatattttac ctgattttat gaagagtttt tctatgagtt atccagatgt aaaattttcc
    25201 ataagccatt atgattctca atatgctata tcagaaattt taaatgaacg aataagcttt
    25261 ggattagttg gttctaaaat caataatcct caaatagagt acctagactt attagatgat
    25321 gaacttgttc ttatcactcc ttcagatttt aaaattgata ataaaaataa ttgtatcgat
    25381 ataggagaat tggcttatct aaattttatt atgagaaaag aagggtcagg tacaagaaac
    25441 ttaatactaa acacacttag taaaaataac tttccagtaa gtaaattaaa tgtaatcgct
    25501 catgtagaat caaatgaggc tattaaggaa atggttagat taggtcttgg agtatccttt
    25561 atatcgtaca tttcagctat cgattatctc aatgcaggta aaataaaatg ttataaaata
    25621 aaggatgttg attttactag aaagtttttc ttcatttact ctaaaaagaa aactttttcc
    25681 ccattagaag ataaatttct aaacagatta tgtgagtatt tcgaaattat aatataataa
    25741 ctttaataaa gtagtgctta attttgagtt tataactaat taataagcta attataacta
    25801 tcacataaat gcttataaat tattatgaac catctttctt ttttatttaa tttagtcaaa
    25861 aaatccctcg ttcaaatgaa cttgggattt ttttatttat actactccaa tagcagccaa
    25921 tactatacaa ataatcataa ctgaaattaa tatcgtagtt gtctttacct tttttcctaa
    25981 taaccaatac ataataccga atatcatagc tggaagcata catggcatta tttggtctaa
    26041 gtaagtttga ataggctctg cccattctcc actacctaca ttaacactaa gttttaacat
    26101 tacattagat gcagtcatac ctccaattac cataagccct aacattgaag ctgcttccat
    26161 aacttttgaa actatacttg aaccactcac atctttaaag aattgagttc catacttaaa
    26221 tcctttatct agacatatat agcgtataat aaaatgagga acatttatta ttaaaaggaa
    26281 taaaatcggt cccattatgt taccctgaga agctaaagct ataccaaccc cagctgctat
    26341 aagctttaat gttccccaga aaaatgaatc tcctatccca gaaactggac ccattaaact
    26401 tgatttaaca gcatttatac tattagcatc aaattcttta ttctttgcat tttcttcttc
    26461 catagctcca gatataccta ccaaaagagt acttatatga ggagtaactg acataaattc
    26521 taaatgtctt tttaaagcct tagatttctt ttctttatca tctttatata atctacggat
    26581 tattggtgcc atcatgtatg catatgctat attttgcata cgttcgtagt tccaagatga
    26641 atctaatgtc aatgaccttc taaaacattt ccaaaagtct ttcctcgtta ttttactatc
    26701 tggtgaaatt gtctctaact tcttagaact cgttgtcatc tactattacc tccttctctc
    26761 ctacaaagtt aacatatatt aaagctattg ctaatccaaa caatgcaact cctgttactg
    26821 ttatgtttag gtaagcaacc aatataaatc ctaataataa aaatgctact gttttcttat
    26881 taagcatcat tctagctaac atggcaaaac ctattgctgg aatcacgcct gctgcaacat
    26941 ccattccatg agttacatat gatggtataa atgaaagtat tttttctatt actggaacac
    27001 ctaatgaaaa tcctatacct actattattg ctaatattaa tttatttcct ataccaaata
    27061 ataattgcac acgttctacg ccttcaatat tcccctcttc tgcataacgg tcagcaatat
    27121 gagctcccca tgtaagtact actgtcatta agaagttatc tataagcaat actagagttg
    27181 ctataggaac tgcaagtaca actgctgctg atacatcttg acccgttaat attacaaacg
    27241 ctgtggcaat aatagaacca gacacaaaat caggaggatt acttgcccca acaggaaatg
    27301 cacccataaa tactaattct aatgttgaac ctattactat acctgtatga agattaccta
    27361 gtgctaaacc aacaagtggt gcaacaacta taggtctatt atgcattgta gaacctacaa
    27421 aactatgcat atatgcaaag aatgcaatta gagttatcaa aataacttgt gtaaattcca
    27481 ttttcatccc cctaaaatta tattaatttc attacatttg tagcatcatc atcaggaact
    27541 aattgtacaa atacatttac gccttctgaa tctaactctt taatcatctt tatatcatct
    27601 tttgatacat gcactgcttt agatatattt tcacgattat catctgattt tgttccacca
    27661 aggtttatag atttaatagc ttttacctct ttagcaagtc tataaacatc ttctacagat
    27721 tcacaaagta tcaataagtt gtatttatca gtaacacctg agttaagtgc ttttatagaa
    27781 tctgctatgc ttttcattac taacttaaca ttagatggtt tagccatttt aagtcctgcc
    27841 attctcaatt catcttttaa taagtcatca cttgctatca atatacagtc tgtgcttaaa
    27901 aactttgtcc atgtaaatgc tacttgtcca tgtataagtc tgtggtctac tcttactaat
    27961 ttaatcatta taattcctcc ctaatttttt tgatttcatc catgaatgtt tttacgtgtt
    28021 ctacactaac atccccaaaa tctttgtaat tatctttaaa gtaacttcct attattgcca
    28081 tatctcctaa tttaagctgt ttttttgcat tttctaatgt aactccagca gctataacca
    28141 atgggaagtc tcctaaattt ttacgaaaca gttctatttt ttccattgat gtttcttgac
    28201 ctgtagcatt ttctgtaact gctatagcat cacatcttgt cattcctatt tttaaatctt
    28261 cttcaacatc attttctgat aatactggct gatatttaaa acgtacgcct ccaattaaat
    28321 acgctgggca ttttgaacgt tgtaatttaa aaaactcttc taaagtagct tcatctctag
    28381 gtttaacatg acctactaca gaatcaacct gtatataact agcattatat tttgttgcca
    28441 gttcaaatcc catagtatca acatttaaac agtttactcc atatggtatt gataaattag
    28501 cttttgatac atattcaagt attctttcta aatcataata attaccatag taattttcta
    28561 acattattcc atctacacca ttttcttcaa agattgtaat ctctttttta gctctttcaa
    28621 agatatcttc tggagtatct ccttttaagt gtatcattgc tataattggt tttttacttt
    28681 taaatactga taatattccc ttttttttca taaactaaaa tacctcttct tcttctttaa
    28741 tttgtaatgt cacgctttgt attcctcttc tagattcttc cactaattgg tctatatctt
    28801 caactgttaa ttcatcagaa aaccttgtaa gaagttctaa taccatattc aaattcattc
    28861 cagaaaataa cctaacatta tgtagattag ttaatcttaa cattgctgta catacacttg
    28921 ctccatataa gtcagataaa attatatatt cacattcttt atcttctgat attgtttttt
    28981 ctaattctaa agcaaaatct gaagcacttt gtccagggta caaaccatac gaactgacac
    29041 aatctgctaa atctcctact atcattttta ctgcatttaa aagccctttt gaaaagtctc
    29101 catgagatgc taatattatc tttttcatac tcatcctcct actaaattat agattatatt
    29161 tctgctgata ctgcatattt gtacttatca cttcttataa attgctcatt gtaatataag
    29221 acatcattat tttctgtgta caccgattgt cttactttca atagtggcat attacttgca
    29281 catttaagat ggtcagaata ttctttgttt gaatgtttta cttcaaatat attttctact
    29341 cgtttaggta atgtgcttat attattagat ataacctcca ttaaagattt atcttctaat
    29401 tcttctttta ataaaaacat gtaatctatt ctaaaatagt caacttcaaa aataacaggt
    29461 acttcatcta ttaatctaag tcttttgata catattacct tctcttctag ttcaatactt
    29521 aaagcttcag ctacttgctt atctgctttt tttattaaaa cagagattat tttggtactt
    29581 ggttttgcat taatacgatg acaagaatta gtaaaactat ttcctgcaca tgttgcttct
    29641 attctctctg gtattgttac ataagtccct ttaccacgtt ttttacataa tatatcttct
    29701 tttacaagtt cattaagtgc atttctcaca gttatacgac ttatattaaa ttctttacat
    29761 aagtctcctt cagatggaag tttatctcct ggtttatata tgccagatgc tatttgtttt
    29821 tttatgttgt catatagttg ttgatataaa ggaacaaagg aatttacttc taaatccatt
    29881 tctttttttt gtctcttttc atccattttg ctcacctccc tatgtaacat ttttataaca
    29941 ttatataaca agttctttat tacatattat aacaagatga aatcgttgtc aatttttttt
    30001 taaataattt ttaatttgac agtattttct tttattttct ctagtgtgac aaattgctat
    30061 ttttacataa aaataagact gttgtacaac agattaaatt gtttatacaa cagcctttat
    30121 tcacttatta catttatatt tttacttata aatttatatt aaaaaatgcc ttatatccct
    30181 tatacatttc atatatgtct actttagaga caatctcatg tggtgcatgc atatttaaaa
    30241 ctccaacacc acagtctata acctctgcac cttgatttgc caatatatgg gctatagttc
    30301 cgcctcctcc ttggtctact tttccaagtt cagcagtttg ccatactaca tttcctttat
    30361 taaaaattct ctttacttct gacataaatt cagcattagc atcattacat ccactctttc
    30421 ctcttgaacc tgtatactta gttaaaacta ctccatttcc catatatgcc gaatttctct
    30481 tttcatatgc ttctccaaaa tttgggtcat atccagcaga aacatcagct gataatacct
    30541 tactatttgc taaagctctt cttactttaa tatcacaata atctccttct aaagcaatca
    30601 gttcagccac agtattttca aaaaactttg agtgcatacc tgtattccct ttagaaccaa
    30661 tttcttcttt atctacacat aatgcaactg aagtaaattc tggttgttca gtttctagta
    30721 tagcttttac agcagcatat gaacataccc tatcatcatg cccatatccc aacaccatag
    30781 acctatcaag ccctaaatct ctagccttac cagctggaac tacttcaatt tcagcactta
    30841 ataaatcttc ttcaactata ttgtatttct catttaaaat ctttaatata tttgctgtta
    30901 taggttcttt ttcttcatct tctaaaggca tattcccaat taatatattt aaagcttctc
    30961 cactaattcc ttctgatagt ttcttttgca tttggtctcc agccaaatga atcaataaat
    31021 cagttacaca aaatactggg tcactatctt cttccccaat agatatgtcc acttttgttc
    31081 catcatttaa tataactata ccatgtaaag caagtggaat agccgtccat tggtattttt
    31141 ttattccacc ataataatga gtcttaaaaa aacctagatt actctcttga tacagtggat
    31201 ttggttttaa atctaatctt ggcgaatcta tatggcttcc tataatcttc ataccttttt
    31261 ctatataatt tttacctatt acaaaaagtg ctactgcctt ctctttattt ataacataaa
    31321 tcttatcacc acaacttatg cttccttttt ccatagcttc atctattgaa atatagttat
    31381 tttctttagc caattttata atttctttta ctgaaagtct ttcagtcttt gacttactta
    31441 aaaactccat ataattgcta gaatattgca taatttgttc aatagtattt tcttttcttt
    31501 caaaatccca agcattctta aattcgtact ttaagttcat attttcctcc tactaagttc
    31561 taatttttat tgtttttcat gtattgctca tattcttctg gtgaaactac tttgacacta
    31621 tctagtcttg atctaaagtg ggctctaaag ttttctaaat attctttcct taaaatttct
    31681 ctttctttga tttcatcagc agaaaggatt ccctctttat ttttctttgc taattcatta
    31741 atcctatcta atttcttctt gtcaaacatt ttttcctcct aaaaaacctc tatttattta
    31801 ataaaataat ttattgcttt ttatatctaa cttgttttta tattcacaaa aaaactcatc
    31861 tatattatca cttattatag atattccctt ttctagttct tccatgcttg ggcgagctat
    31921 atttatccta aagaatctat catccactag attgtcaaaa aaataagtcc ctggaagcat
    31981 tgacactcct ttgtttaaca taaaatttgt aaatgcttga gatgtatatc ctcttggtaa
    32041 ttctaaaaaa aagtttattc ctccacaaga ttttctaact tttaatttac cactcaattt
    32101 gtcattaatc aagttttttg ccaatttata cttatcagta tatatcttct ctatatgact
    32161 caaataattc ttccaatcaa aattattcat atagtaatac attgattttt gaattaaacc
    32221 tggtgttgat atatctgatg aatatttagc ccataaaact ttttttaaca actcatttgg
    32281 catttctaca attcctatcc taagacctgg cataagtatt tttgaaaagc tttttatata
    32341 tactactcta tttttatcat cataacttct aattgttcta ttgtcctttg attcaaaaac
    32401 aaaatcactt ataaaatcat cttctattat ataaaaatca tgttcttctg ccaattctat
    32461 caatttcttt tttttatatg ttgagtaaga tattccagtt ggattttgaa aattaggcat
    32521 aatgtatata agtcttggtt ttattttttc caactttaat tttaaaatac ctatatcaat
    32581 tccatcatct aacataggta tagatattat ttttgcacct ctacttttaa acacttcaat
    32641 tgctccattg taagaaggtt cttccataaa aacaacatct gaataactta taagtccttt
    32701 acaaactata tctatacctt gttgagcacc tgatattatt tgaatatttt ccttagtagt
    32761 ttttatattt ccatcactta gatactctac cattttttct cttaattctt cagagccaag
    32821 accttcatca tattcaaata ttgaactacc atctttagat aatgccatat ttacagcatt
    32881 tttaaatgaa tcgataggaa acatatcatt agatggattc ccattgtcaa aatgaattaa
    32941 atcttctctt tgcacaataa tcttattttt cctgttatct ataccgcttt tataagaatt
    33001 taaacttgat acaaaagtac cactccctat aattttatat acatatcctt cattttctaa
    33061 aagctcgtat accttaacta tagtagtgtt atttacttgt aaaaaatttg atagttttct
    33121 tattggaggc aatttttcat gttctactaa ctctttattt attatcaatt cttttatatg
    33181 attaaatatc tgtatatatt tagttgattt tttctcattc aaatctattc tgtatttttc
    33241 caaatttatc accgctcatt gtatttatat tttattgatg cttttatgta tatttgatat
    33301 tatttagtat gtatatataa taccaaaatt tttataaaaa ggggaaatat ttatgtttta
    33361 cgactatcac atgcactcaa gtttctctac agacggaaaa tctacattgg aagaaatggt
    33421 aaaaaaatct attgaactcg gtttggagga aatttgtttc acagaccatg ttgactatga
    33481 tgtatatgct gatgattctt tttcaattgt ctacgaagat tattttaaga gtttagagac
    33541 tcttcaaagc aaatataaag ataaaatatc aataaaaaaa ggaattgaat ttggagttca
    33601 aactcaatta atggatacat acaaaaagga agcacatcaa tatccattgg actttataat
    33661 atgttctatc catgctatag atacaatgga cttatacctt ggtaattatt ttaaagataa
    33721 aactcaacat gaagtttatg aaaattacta tctttattta tataatattg ttaagaatta
    33781 taaagattac tctgtcttag gtcatttaga tttgattaaa aggtatgcac cttatgacac
    33841 tattttagat gatagactat tttctgatat aattgaagaa actcttaaac aagctattta
    33901 tgatggcaaa ggtatcgaga taaatacctc ttgttataga tataatcttc cagacctaac
    33961 cccatctaag tatatacttc aaatgtataa agacttggga ggggaaatca taaccacagg
    34021 ttcagattca catcatataa gtcaagttgc atgtgagttt gactacatat attcattact
    34081 aaaaaatatg ggatttaaat atgtatctaa atttaataaa ttgaaaccag aatttataaa
    34141 attataaatt tttacacctc cttgtgaata tatttaaaag ataaggaggt gatttttttg
    34201 aaagtacctt atttagtact atcaacagtt attttattta tatccaattt tattgtaaga
    34261 atatttggtt ttttatataa aatattctta tcaagagcac taggtgaaac aggtttaggt
    34321 atctatcata tgatttttaa ctttttgatg atttgcttag ctgttacaac aacaggtata
    34381 cctactgcac ttagttgtct agttgcaaag agaaaagctt taaacgatag acacaatact
    34441 aatgctttat ttatatcaac tttatatata tcatttttcg tagcactaat tatatctata
    34501 gttgcatctt tcaatagttc ttttttatca cttaaatttt taaaagatgc taaattaaat
    34561 ctatttatac tagctgtttg tcctgctata gttataataa ctctttcaaa cgtactaaga
    34621 ggatattact atggcataaa gaatgtaaaa atccctgcta ttggacaaat cattgaacaa
    34681 attggtaaaa tattatttgt ttttttactt gttatgtaca taaataataa atctatgaat
    34741 tgttatatag ctctattagg tatatcgatt ggtgagttga gtaatattat atttatgctt
    34801 atttgcttat ggcgagattc atcatttgac aataggtata ttataagcat aaaagatttt
    34861 tataattctt ctatggaaac attaaaaatg tctattccca taacttgtaa tagaatgtca
    34921 aacatacttt tgcaatctat aagttctatg atgataccat caagattagc attatcagga
    34981 atgacttatc aacaatcttt aagtatgtat ggtattgtca gtgggatggt aatgcctttt
    35041 atatttttac cttttactgt tggttcagcg cttattgtaa atttaatacc aactatttca
    35101 caggaaatgg ctctaagaaa gcgtaagagt gtaattaaaa aaatcaaata ctccatactt
    35161 cttactttat ttgtaggcat actatcatca atattttttt atttctttgg aaaagattta
    35221 tgtatattag tatttaaaaa tacacttgca ggagaatatc taaaagctat gtttttagtt
    35281 ccactattta tgtccctaaa tcaaactcta tctggcattt tacattcaat aagaaaagaa
    35341 ttagcttcaa gtataaatac tattactggc atgttaattc aactaatagc tttatatgta
    35401 tttcttccaa ttccaggact taatatttat gcatacatat acacaatgac gatagtatca
    35461 atctttactt gtctattaca tactatagtg ctttttaaat cactaaaatc tatacgctag
    35521 ttataaaaac gtgaaggcta ccaatgtaaa ttatacactt tggtagcctt ccttagatga
    35581 tttttattta taatttaaac tgtctatata tcttacctat ctatctgtgg caacttggct
    35641 ttccacttcc atgattaggt ccacattgcg aaaaatcaca tactttagag aaagtatcaa
    35701 caagtcttgg ttggattgga tttggtgatg ctaaaactac tatttgttca cttattaatg
    35761 tgctaactgt ttcttgtaag caaagtacta aatctatatc atttggtata attaattcat
    35821 agaaaggttc acaacattcg ttgcattcta attttaatat agattttcct atacaatcta
    35881 cagttaagtt agtttgatat tcttgtctta aagtaacagc tcttccgtca gctggcaagc
    35941 atattggtgc tgaaagtgtg ttaaaagtaa attctacata atcaactctt ctcttacatc
    36001 cacctcctgc tctaactgct gggaaagcta atgcaacgaa ctctgtacat ccacatctac
    36061 ctcttagctc tagtactaaa ttacgaactg ctacagttaa tcctctttgt ttataagcta
    36121 ctgatttgcc ttttccttgt ctacagcact cagcgtttct ttctccacaa actgcaaatt
    36181 caaaagttgt ttcacagttt cttcctacta tatctcctaa tgggtctaaa tcgaaatctt
    36241 ctaaagttgt cattcctgtg tctataacta ttccatcatt acttaagcat attttttcta
    36301 tagttacact tgcttgacct gcacttggaa ctgaaccaaa tacttctact acttctgttt
    36361 caaaagttaa tggctctcca tttggtcctg ttgcatctgt aaatgtttgg aattgcatag
    36421 tatcaaatat tctattagcc tctattgcaa atggcactgc atttgataca gagaagtttc
    36481 ttggagatat aggattacag cattctggtc cacatttgtt ttcaaaacat tcatctgaat
    36541 ccatttcaca tggttcacaa tcacatttac aattgtcatg gcatccacaa ttgtcatcac
    36601 atggattagg tttgcatgga ttgcatgggt taggtttaca tggattacat gggttgcatg
    36661 gattacactc atcatggcac tcacacttat tatcagtatt acatccatta tgatggtgtg
    36721 attttttaaa ccctctcatg tcttctgaat ttatttcatc tgtatacact tcttcttcat
    36781 tcattgtaga cattggctga ttcatcattc ttcttttatt ttttttataa tcttgcattt
    36841 ttcattgccc tccctgtaag cgattcttat taagtctttg gctttttgaa gcctaattaa
    36901 tactatgctt ttataatctt aattgttact tgctttttaa atttatcaat aattaggcgt
    36961 atttagtaga aaaatgcatt ttaataaaaa aagtactact atttgaaata gtagcacttt
    37021 ttttgtcccg aaacaccggt ggctaagtaa attcacaccc taggcactcc caggtgggtg
    37081 acttgccagt aacctttaac gtccaaacta actaggcatg ttataattta ccagacacgg
    37141 tctcccgtat ttcatttgta ggttgaattt aaaaaagcct tatcgtatgt ttcaggattt
    37201 catattatta ttatataata aattttgaat tttttaaagt ggttttatat agtaattgag
    37261 ataatcttat tctatcatta ctttttcttc cttttttctt tatgtattta gttaattatc
    37321 gttttttctt agctctagtt ttagtatttt tttcattata attgtgttcg gattcttgct
    37381 cattaatgtt gtatagttcc tcatcatata ctatatcttc attattttct atattttcat
    37441 agcctgtgtt cttatcttct actaattcat cttcattagc ctcaatttcg ccttcgtctg
    37501 ttaactcatc ataatcataa tccaattctt tatcttcaca atctaattcc tcatcttcat
    37561 aatctaactc ttcattatcg tcttcatcat cattattact atcatcacct cttaaaagac
    37621 ttcctaacaa tttgagatta ttcatactcc caagattgtt tatgtttgcc aaagagctca
    37681 aactaaaacc gccttcacca aaatcatctg gtaaaaataa ttctgcaaat aaatctatct
    37741 tctttttcat tcctatagct ttttcaactg aattttttct tttttctggg aatacatcta
    37801 taatctcctt tatcatcatg ttctttttat catcactatc catttttctg aaaaaatccg
    37861 tttcatcttc tatatctact atatctatat tatttaattt ttttattcct ctaactaaat
    37921 ctaaaatact ttctattttt attagtattt ttctttcatc tcttgacatg tattttttag
    37981 ttcttgttat tatttccatt cccatttcta aatcttcttc acttaaatcc atgagcctta
    38041 aacctctttt tatgtcattt gcatcaaacc ttaaattgcc atgtgtagac tgtgacagtt
    38101 tatcatttgc attttttttg ttttttctaa ctctattatt tccttttgat ttatttttat
    38161 catcaaatat agagatatta ccattactca atgcaatcac tccaatcaaa aatagtaagt
    38221 tattcaagac aattccccct tatattatat aattgcccta tattaaagta tgtttttcat
    38281 attttaatgt tccattt
//
LOCUS       DACXMZ010000011        36822 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.011, whole genome shotgun sequence.
ACCESSION   DACXMZ010000011 DACXMZ010000000
VERSION     DACXMZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.011"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(22..1977)
                     /locus_tag="JD016_02180"
     CDS             complement(22..1977)
                     /locus_tag="JD016_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998436.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIDKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDDSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(2160..3635)
                     /gene="spoIVA"
                     /locus_tag="JD016_02185"
     CDS             complement(2160..3635)
                     /gene="spoIVA"
                     /locus_tag="JD016_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HAU4998437.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIVQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3953..4984)
                     /locus_tag="JD016_02190"
     CDS             complement(3953..4984)
                     /locus_tag="JD016_02190"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAU4998438.1"
                     /translation="MVLNMEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKI
                     NRTKENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEG
                     QVIVNVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDL
                     KIAQMIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMT
                     RGISEMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLK
                     EVKMVVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEM
                     EEVVLGDDI"
     gene            complement(5003..5617)
                     /gene="plsY"
                     /locus_tag="JD016_02195"
     CDS             complement(5003..5617)
                     /gene="plsY"
                     /locus_tag="JD016_02195"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAU4998439.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLICLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(5652..6977)
                     /locus_tag="JD016_02200"
     CDS             complement(5652..6977)
                     /locus_tag="JD016_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAU4998440.1"
                     /translation="MSISRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRI
                     FAEVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAA
                     DKEIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLD
                     EIVENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAID
                     TYFEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTE
                     QDTKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAK
                     TNQRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTD
                     IRPPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(7005..8342)
                     /locus_tag="JD016_02205"
     CDS             complement(7005..8342)
                     /locus_tag="JD016_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HAU4998441.1"
                     /translation="MKILEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIH
                     DIIEYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCM
                     FCFIDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVH
                     TTNPELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQ
                     LYPYVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFL
                     SDEFYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSI
                     ATGHSAYEFIQSMADAMMDKFKTLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDK
                     ELGEALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(8571..8771)
                     /locus_tag="JD016_02210"
     CDS             complement(8571..8771)
                     /locus_tag="JD016_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998442.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(8773..9705)
                     /locus_tag="JD016_02215"
     CDS             complement(8773..9705)
                     /locus_tag="JD016_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore envelope protein"
                     /protein_id="HAU4998443.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(9750..10682)
                     /locus_tag="JD016_02220"
     CDS             complement(9750..10682)
                     /locus_tag="JD016_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998444.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(10737..12026)
                     /locus_tag="JD016_02225"
     CDS             complement(10737..12026)
                     /locus_tag="JD016_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAU4998445.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYISKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(12055..12747)
                     /locus_tag="JD016_02230"
     CDS             complement(12055..12747)
                     /locus_tag="JD016_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4998446.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(12762..13523)
                     /gene="pgeF"
                     /locus_tag="JD016_02235"
     CDS             complement(12762..13523)
                     /gene="pgeF"
                     /locus_tag="JD016_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HAU4998447.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVHEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(13520..13981)
                     /gene="nrdR"
                     /locus_tag="JD016_02240"
     CDS             complement(13520..13981)
                     /gene="nrdR"
                     /locus_tag="JD016_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAU4998448.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(14003..14266)
                     /locus_tag="JD016_02245"
     CDS             complement(14003..14266)
                     /locus_tag="JD016_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HAU4998449.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(14370..15143)
                     /gene="sigG"
                     /locus_tag="JD016_02250"
     CDS             complement(14370..15143)
                     /gene="sigG"
                     /locus_tag="JD016_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HAU4998450.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(15235..15957)
                     /gene="sigE"
                     /locus_tag="JD016_02255"
     CDS             complement(15235..15957)
                     /gene="sigE"
                     /locus_tag="JD016_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HAU4998451.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(15982..16827)
                     /locus_tag="JD016_02260"
     CDS             complement(15982..16827)
                     /locus_tag="JD016_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAU4998452.1"
                     /translation="MVYIEYYVIENLVINYIIISCTSILTKRYNPKKNKLLGASLGGA
                     YSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLVNIFISGSTY
                     FIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTININLLEKSFCC
                     KALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQEIIGSLDDK
                     TSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDREYGAIMNPN
                     ILPGF"
     gene            complement(17144..18418)
                     /locus_tag="JD016_02265"
     CDS             complement(17144..18418)
                     /locus_tag="JD016_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAU4998453.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(18702..19862)
                     /gene="ftsZ"
                     /locus_tag="JD016_02270"
     CDS             complement(18702..19862)
                     /gene="ftsZ"
                     /locus_tag="JD016_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAU4998454.1"
                     /translation="MMLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISV
                     NTDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFV
                     TAGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVD
                     TLITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTI
                     MKDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEV
                     NEASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSI
                     RSSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(20049..20402)
                     /locus_tag="JD016_02275"
     CDS             complement(20049..20402)
                     /locus_tag="JD016_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1290 domain-containing protein"
                     /protein_id="HAU4998455.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(20501..21175)
                     /locus_tag="JD016_02280"
     CDS             complement(20501..21175)
                     /locus_tag="JD016_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HAU4998456.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VDLFAK"
     gene            complement(21180..21878)
                     /locus_tag="JD016_02285"
     CDS             complement(21180..21878)
                     /locus_tag="JD016_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HAU4998457.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(21901..22641)
                     /locus_tag="JD016_02290"
     CDS             complement(21901..22641)
                     /locus_tag="JD016_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAU4998458.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(22911..24140)
                     /gene="murG"
                     /locus_tag="JD016_02295"
     CDS             complement(22911..24140)
                     /gene="murG"
                     /locus_tag="JD016_02295"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAU4998459.1"
                     /translation="MMKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIE
                     SEIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGG
                     YVSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDK
                     LVFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVN
                     EDIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISL
                     AEITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLG
                     DRELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKE
                     TTPSIEGQAKVIGIKKR"
     gene            complement(24155..25285)
                     /gene="spoVE"
                     /locus_tag="JD016_02300"
     CDS             complement(24155..25285)
                     /gene="spoVE"
                     /locus_tag="JD016_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HAU4998460.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(25337..26692)
                     /locus_tag="JD016_02305"
     CDS             complement(25337..26692)
                     /locus_tag="JD016_02305"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAU4998461.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(26711..27679)
                     /locus_tag="JD016_02310"
     CDS             complement(26711..27679)
                     /locus_tag="JD016_02310"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAU4998462.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(27720..29093)
                     /locus_tag="JD016_02315"
     CDS             complement(27720..29093)
                     /locus_tag="JD016_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAU4998463.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(29260..31239)
                     /locus_tag="JD016_02320"
     CDS             complement(29260..31239)
                     /locus_tag="JD016_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HAU4998464.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(31251..31598)
                     /locus_tag="JD016_02325"
     CDS             complement(31251..31598)
                     /locus_tag="JD016_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998465.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(32079..33014)
                     /gene="rsmH"
                     /locus_tag="JD016_02330"
     CDS             complement(32079..33014)
                     /gene="rsmH"
                     /locus_tag="JD016_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAU4998466.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(33044..33790)
                     /locus_tag="JD016_02335"
     CDS             complement(33044..33790)
                     /locus_tag="JD016_02335"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAU4998467.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(34184..35470)
                     /locus_tag="JD016_02340"
     CDS             complement(34184..35470)
                     /locus_tag="JD016_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAU4998468.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYINYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDVTFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(35485..36750)
                     /locus_tag="JD016_02345"
     CDS             complement(35485..36750)
                     /locus_tag="JD016_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAU4998469.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
BASE COUNT        11206 a         6963 c         3653 g        15000 t
ORIGIN      
        1 taaataatac ctttctaaaa attatttttt taattttatg aatttaatta tactttttct
       61 agtatcagga tttacaatta atatagaacc aatcataatt ggtgttagta tacaaaataa
      121 aaaaataata tcttttttta gattatattc tttagattct aatttataat tattatttat
      181 tattccatca taatcactta tatttgactt atttttattt gtatcttgat ttttgttaga
      241 aatactttcc ctataaggaa aagaatgttc attggtacca aatactgatt ttgagcttaa
      301 atcataatta tcatcatttt ctgtaattga atttttattg taaaatcctt tttctttatc
      361 agaattattt tctttattac cgtctttagg tatatcatga ggatttttat tttcattgct
      421 atcagtactt gattctacat catctttagg agtatttggt tgtttctcat cagaatcatc
      481 attttcatta ttattatttt cattattatt gttttgattg ttattactat cattattttc
      541 atttccatta ttcccatctt gattgttatc atcctcatct ctatccgggt cgtctctacc
      601 tcctcctcca cggtcaccat caatatcatc attttcccgt tcatcatctg gtgtctcttg
      661 gttgtctccg cctcccatta taaatcctgg tttaaaaact atatttgaat acccttcttt
      721 taatcctcct acaactttta ctctaaaaca acgcaactta aaatcttcaa aaaaaagtat
      781 agcttgatct ctaatatcct cctgttcttc agatatccaa ttgattccat cgcttgaata
      841 ttcaacaaaa accttacttg cactatacgg catatcataa aataaaacag ctacatatcc
      901 attttttgta ttttgaaatt ccagttccaa attgtctatt ggttttttat ctataacaga
      961 tacattcaaa ataggaacta catcatcatt tattataatt ttttctttat ttaacatttc
     1021 tgttttaaac tttcctgtta tattaaaatt ttctcccttt tccaactttt cataataatc
     1081 agtaaaatcc cattctacat ctatatcaag tcgattacca ctagatgttt ctaataccat
     1141 atatttggga aattttatat tttctatttt atcatcttta cttactttta tttcatcact
     1201 aaatacacca attcccctat gacaacattt cacaatattg tagccagaat cttcagctat
     1261 agaaggtata agctcattat aatcatcaat tatataaact ttcttacttt ctgatataat
     1321 tgattgtgcc aacttattat tatcttttag taacccactt tcattgttgc tactatatgc
     1381 ttttattgaa gtttcttcta tctcaacatc acctttagaa taaacaccta tagcatgttc
     1441 tccattcact tcaatatcct tatttagtat tctaatatca ttttcagtat aaattcctat
     1501 tgcatctttc ccatttgcct ttattttttc aacagattcg taggatgatt taatatgtaa
     1561 gtctcctcct tcttcaacgt ataaggctgt cccatcagta gcaattatgt tgtttatacc
     1621 aatacttata cttccaccat tttctatatg tattactcct tcttttcctc cttcacctat
     1681 tatattcaat tcattatcat caataataaa tctcccatga ttttttatca atattttata
     1741 tttgttggta tctattgtaa aattactgtc ataaggaata taaaatctaa actcttcatc
     1801 tattaccata tcacaagaaa gagtataaac tatttcttct ttattatttt tattttcttg
     1861 aacccacctt tcaaaatctt caagacaatt ttcctcttgt ccaaaaactt tatgtggaaa
     1921 acttataaaa cacaaaacta ttatactcat aaaaataaat atctcatact ttttcatctt
     1981 acactcctag aaatttttag ctttgaaaca atcctgtcga aacatatact tcacccacta
     2041 taatttttcc acaattatca attattgtca atttatataa gtatctttat taattgttat
     2101 aaataaaaag ctacttacta aagcaaacag acgtttcact ttaataagta gctatatttt
     2161 tataacaaaa tagttataat attagaactt ccttcattta caattttttg taaagtcttt
     2221 tgcattttaa ctcttatctc ttctggcata gtatataatt tactttgtaa ttgctctttt
     2281 actaagtcat gtagtgactt accaaacata tttgattccc ataaatctgc tggttgctct
     2341 tcaaatactt ccataagata ttttatcatc tcttcacctt gattttgatt tccaactatt
     2401 ggagaaacct ctgtagatat atctgctttt ataatatgaa gtgatggtgc attggctttt
     2461 aacttgatac catattgttt tccttgtttc attatttctg gttcttccag actaagttct
     2521 tctaaagaag gagctactac tccatatcct tttatcttgg catctattaa agcactttct
     2581 attttatcat actcattttt aactttagat agtctagtta tcagactaag cagttgataa
     2641 tctccttcaa ttttaaatcc acttttttct tcaaggacat tatagaataa atcttgcttt
     2701 gtctgtaaat caatatttat aacaccttct cctagctcta cattatcaac acctgtatct
     2761 tccaaaaact ctaactcaga aaatccttgt acaatacctt ctatatccct tattttacct
     2821 atatctataa tactttgttt taatgttgtt attatgctac tttttatcca atgatttctt
     2881 tcaagacctt caacccattt tggtaagttg attcttattt ctgtaagtgg aaaatcatat
     2941 aatacagctt ccataacctc ctctatatct tcttcttcca tttcaaccac attcattgga
     3001 agaactggaa cttcatactt ttcttccaat tcacttctaa gcatactcgt ttcctcactt
     3061 tttggactta acgtatttaa aaccactgca aatggttttt taagattttt caattcttgt
     3121 ataactctct cttctggttc tacatagctt tttctgtcaa ttccagtaac tgaaccatct
     3181 gtcaagacaa ctatccctat tgtagaatgg tcttttataa cttttttagt tcctatctct
     3241 gctgcctttt caaatgtcat agcttcttgt gaccaaggag tggatactaa tctttgtttt
     3301 ccaccttcct catgccctaa agctccttca actatgtatc caacacaatc taccattctt
     3361 acttttaaag atactgtgtc ttttattttt atttctacac catctgctgg tacaaatttt
     3421 ggttctactg tcattatcgt tttacctgaa ccactttggg gtatctcatc tcttgttcta
     3481 tcctttttaa attcattgtc tatattaggt ataaccaact tttccataaa ttttcttata
     3541 aaagttgatt ttcctgttct tacaggtcca actactccaa tatatatatc cccctgagtc
     3601 cttttggata tatcttcgta tatgttatta ttcatattat tcctcctata catctaatct
     3661 tattaaatta tatttagagt taagggttaa tattcttaaa atagaaaaat aaagactagc
     3721 aatattaatt tcccctatat acaaaaacta gtaaactaca atgctaattg aaattattaa
     3781 aatccattac attaaaagac ataattatag tcatgataat tacaaattat atataaaata
     3841 atttattata tcgtatatac atagactatg ctaagtaaaa ttaaaatagc cctctttata
     3901 ttaaagaggg ctaaaacaaa tctattattt ttttatttta ttatacaata gtttatatat
     3961 catcacctaa aactacttct tccatttcat gctttttact tctcatcatt agttctattc
     4021 caacttcttt aggatttgaa cctttcttta ttacagaata tatagcatta gttataggca
     4081 tatctatatc taatttttca gcaacatcat gtgctacttc agtagcagtt atgccttcta
     4141 caaccatctt tacttctttt aaagtatctt ctaaactcat acctttacct atgagtatac
     4201 cagctcttct atttctacta tgcatacttg tacaagtaac tattaaatct ccaatacctg
     4261 aaagacctgc aaaggtagac atattagctc ccatagcaat tccaagtctg ctcatttcac
     4321 taatacctct tgtcataagg gcagcttttg cattatcacc ataccctaat ccgtcacata
     4381 tcccagctcc aaatgctata atatttttta atgctcctcc aagttctact ccaactatgt
     4441 ctggattcgt ataaactcta agtttagggc tcataaataa atcttgtatc atctgtgcta
     4501 tttttaaatc tttagaagca actactacgg tagtaggaat atcccttgct acttcttcag
     4561 catgagatgg cccagatagt attacatatg gattttgtgg taattcttct tcacagactt
     4621 gagataatct aagcccagtc cctttttcaa gacctttagc tacattaaca ataacttgtc
     4681 cttcttttat aaatggtttt attttttggc aagtacttct tacagcctgt gatggaactg
     4741 ctaaaactat taccttacaa tcttttatag ttttttctat atcagtactt atagttatat
     4801 tatttggaaa taatactcct ggtaaatagt ctgtattttc tttagttctg tttatttttt
     4861 tagcttgttc ctcttttcga gtccacatgc tcacatcatt accttttttt gcaagcccta
     4921 aagctaatgc acttccccag cttccagtac ctaatacaca tactttctcc atgtttaaca
     4981 ccacctgagt ataatatatt tactattctt tcttttgacc aatttttctc tctgtcccat
     5041 ttaataatcg ctttatattt gctctgtgat tatatattac tgatgcagtt aagaacagag
     5101 ctactattaa accagcttta ttttttacca taatcataaa tattggtgaa catcctattc
     5161 ctacaacaga acccaatgaa acatactttg ttatagctac caccaatata aatactgcta
     5221 gacatattag tgttattact ggatttacag ctagcattgc ccctagtgaa gttgcaacac
     5281 ctttgcctcc tctaaatcct aaaaccgctg gccaattatg acctgctaca acacatataa
     5341 ctgccagata tccagctaaa agagtatcta ttccaaccaa tcttgctgca aattctgata
     5401 ttaaaacagc cattacacct ttaagaacat cacctaaaaa tgtcattgct cctgctcttt
     5461 ttccaagtgt cctaagtaca tttgtcgaac ctgcattacc agaaccttga gtccttatat
     5521 caaccccagc aattcgcttt gcaactatgt atgaggtaga tatatttcct aaaagatatg
     5581 caactacagc aataattata taactaaata tctccactga atttcccccc tttagttata
     5641 ttttaagata tttatttttt atttttttgt ctatactcaa actttatgga agttccttca
     5701 aatccaaaat tttctcttat cttattctca aggtatcttt gatatgagaa gtgagttaaa
     5761 tccttatcat ttataaacaa tgtaatttta ggaggtctaa tgtcagtttg agtcccatag
     5821 taaattttta atcttctacc tttgtcagat ggtggttgat ttaacattac agcttctcca
     5881 attacttcat tcagtgctga agttgatatt ctcttagaat gttcatttga cacatattca
     5941 actgtatcta gtatcttgtt cattctttga ttagtttttg cagaaacaaa tacgattgga
     6001 gcatacatca taaatggaaa tttttctttt atatcttttg tgtaattact catagtctta
     6061 ttatctttct ctattaaatc ccatttattg attacaaata tgcatccttt tccttcatca
     6121 tgtgctatac ctgcaacctt tgtatcttgt tctgttacac cttctaaggc atctattact
     6181 atcaatacta catctgctct atctacagca ctcatagctc ttattacact gtatttttct
     6241 attgtctcat atattttact ttttcttcta agccctgctg tatctattag aagaaatttc
     6301 tgtccatttt tttcaaaata agtatcaatt gcgtctctag tagttcctgc aattggactt
     6361 acaattactc tttcttctcc aagtatctta ttgagtatag aactttttcc tgcatttggt
     6421 ttacctgtta tagctactct tattatatct tcttcatact ctgtatctaa tcctgctgga
     6481 aaattttcaa ctatctcatc taataaatct ccaaatccca tactattggc tcctgaaact
     6541 gcaaaaggcg ttccaaatcc caactcataa aaatcgtata tattatcaaa ttgactttga
     6601 ctatcaatct tattaaccac taagattact ggtttttttg tctttctaag caattgtgca
     6661 atttctttat cagcagctgt tattccagct ttaccatcaa ctataaaaag tattacatgt
     6721 gacatgtcca ttgcaagcat ggcttgattt ctcatttgag ataatattat atcttcgcta
     6781 tcaggttcaa ttcctcctgt atccactaat gtaaaatatt tatctaacca ttctacttca
     6841 gcaaatattc tatctcttgt aactccaggt gtattttcaa ctattgatat cctttttcct
     6901 gcaaatttat taaatattgt agacttacca acgtttggtc tcccaactac agctacaact
     6961 ggcctactta tactcatttt atttctcctt ccatcttttg ttttctattt taaaatttta
     7021 tccacaaaat cttttccttc attcaaacaa gggataattt ttattctcat aagttcttct
     7081 aattgattta attcaatatt atctaagaaa atttcttcat ctgcttttag catacttctt
     7141 gttatataaa gagcttctcc taattcttta tcttcaagtt gttcttttaa atccttagct
     7201 gttaaaagac ctgatacagt tattgtctct ccaaaaaact tgtttttgat ttcatataca
     7261 tttacttgaa gagttttaaa cttgtccatc atagcatctg ccatactctg tataaattca
     7321 tatgcagaat gaccagtagc tattgatact ttcttctctc tttttagaat attctcaggc
     7381 aatatattta gatactcttc aatctctgtt tttaacttac gtatcatacc tacaccatct
     7441 tcaaactgta aaaaaccttc atattcttca tatccaggca attcactatt agatagtata
     7501 taaaattcat ctgataaaaa tgcaaatcga gtacctaatt tttcaagata tttctgttgt
     7561 atttgatgta tttgctctat agttttactt gcgctcttat cattaaatat atttaattct
     7621 actagatttt ctctatgctt tgtaatccca actggaacta tcgcaacact gtttacatac
     7681 ggataaagtt gagctagttc ttttattgtt ctatccaatt cctttccatc atttatacct
     7741 ggacataaaa caatttgaca gttcatttct atatgagcat ctgccaatct cttcattata
     7801 ccatataatt ttcctgcaaa tttattgctt atcatttttt gtcttaactc tggattagtt
     7861 gtatgcacag atatatttat aggacttatt ctatatttta taatattatt tatatcttct
     7921 tcactcatat ttgtaagtgt gacaaaattt ccttgtaaaa aagataatct cgaatcatca
     7981 tctttaaaat aaagagtttc tctcatacct tctggcaatt ggtctataaa acaaaacata
     8041 cacttatttc tacagctttt agcttggtca attatcggat ttgtaaattc aacacccaaa
     8101 tcttcatcat agtctttttc tatctcgtat atgtagactt cgccatcttt tttttgtatt
     8161 tctacatcta aatattcatc acttagtaag aatctatact ctattatatc atgtattggc
     8221 tgttcattca cagatataag taaatctcct acctctatac ccatttcttc tgcaatacta
     8281 tctttataaa ccttacttat aatattattt acttttttta catttacttc caatattttc
     8341 accactcttt tgtattcatc aatgcttatt ataatgtatt ttaatatgca atttatttat
     8401 ttattatatt ttatgatagc atttttaagc atttcatgtc ataataacac attttaattc
     8461 taatagtcaa tataagtaaa tagctataaa atagattaga acaaataata ttcaaattta
     8521 agtttcttaa aaatttaaat attatttgtt cttaaaaaaa ttttataaat ttaatgtttt
     8581 actatgatta acgtcccatt tgtcatatca tgaaccattc cacctacaca ctttgacatc
     8641 aacaatgcct tattttgtaa gtctttctca tcatttgcat aaaatattgg acaacctcca
     8701 ccatttttca tattcttatc aagtgtaact atagctaggg tatattcatt taaacctata
     8761 tccattccca tcttatgcac ttcctttctt tcctatttta taatttttta gtgataagtt
     8821 ttttccttta gagcttgaaa gtattgggca tgactttaca gcttccatta ctttttccac
     8881 attcttatct actggtaaat aagcaatcat tatactttca tcctctttat ttcttctagg
     8941 taatggtacg aatgaaggtt ctccataatc tccatataaa cctaaccttg aatatatatt
     9001 gtatactatt gcttgtcttt gaccggagtc aaataatatt cctgcattag cataatcctt
     9061 atttttagga attatttgta ttcctacacc tctattaata tatacttctc tatcgctttt
     9121 tagacctata tttgttatac cttttaaatc tcccaccttt agtatagatt cgtccacaaa
     9181 actaatttca gcaggcatta catcagctat gtctccaata ctttttctag taagaagttt
     9241 ttttaatata aaggctaaca ctaatccaca aattgaactt attaccaaac tcatcccttc
     9301 acttattttc gtttctgcaa gtattacata atacaatcct attgttaaaa aagatgtaac
     9361 tatacacata taatttctta cttcataggt tctagctatc tcttctatga atgcagctcc
     9421 tctctttaca agttgaactt cctctaaatt ttgcaatgta tttcttctat ttgctctcac
     9481 ttgtctaaat tgttctgctg ctagagataa aaatgttatg gatgtataag aacgatttat
     9541 aagagctgga accaataatg ctcctaatga agaagcgacg aatgctaata taagttgaga
     9601 tagtaaaaca tttggttgcg ttggatactg tttttggtct aaattcaaca ttataattct
     9661 agatgttata ccaataataa cagcaacaaa aaatactcta tccattaaat cactcctaaa
     9721 atatttaatt tgaaatttta catgtaaatc tacaaagtct ttctagaaaa atcactctgc
     9781 tttccttttg atgtttctat gataggtgta cttttcacta catctatcat gacatctata
     9841 tctttgagta ttggcatata tggtataacc acagtacttt tttctaaatt ggtcttagat
     9901 atagcaacaa tatcaacctc atctacatct ctatctatcc ccatatgaat aaatagatta
     9961 tgtattatag cttgtctttg ccctatatca tttactattc caaatgcacc taaatctctt
    10021 ggtataactt ctatagccag acattcattt ctatactttt ctcttgtact ttctagacct
    10081 atgttagtta ttattacacc atttaccatt aaaataggtc cttcaaaact tatttttgca
    10141 ggaactatat ctgctatatc tgctatactg ttccttctta aaaatcttct aaaaatcaaa
    10201 ccaacgatag ctcctgacac aatagctaag attgtgcaat aaaaaaagct aaggccatac
    10261 tttcttgcaa aaacgatata aactatcgaa gataccagtg cagaaaaaag acttatataa
    10321 cttctagatt catacgtaga tgctatttct tcaacgtaag cgtctccttt tggtactaat
    10381 tcttcattat cgatattctt tagtgttatt ctttcttgtt ctgcaagacc ttgaaactgt
    10441 tgtattgcta ctgcaaaaaa tgtcaaggct gcaaactcct tatctataag tgcaggtaat
    10501 gctatagcac ctaacgaagc tgaaagccca gatataatta tctgttctaa ataatcttgt
    10561 ggtcttgatg ggtattgttt gtctgtaact cttaaaacta atcctctaca taatattcct
    10621 acaactaatg caactataaa agagtgtcta aataaatttg cattcaataa actttcttcc
    10681 attttctcag ctccttctct tatatagttt gtgcaaaaaa caaaaaaaat tcccttctat
    10741 ttaaggaaat ttttttgtaa tattgtaact ataaattcgc taccaatgcc aagttcactt
    10801 tttactttta tacatccatt tagtgatttg actatatgct ttgttatagc caatcctaaa
    10861 cccgttcctc ctacatccct acttcttgct ttatctaccc tataaaatcg ttcaaatatg
    10921 cgttctacat cttcttttgg tatccctaca ccattgtcta taactttaat tacgattttt
    10981 tcttcatctt ttaacactac aacttgtaca atcccattat cttgggtata ctttatagca
    11041 ttatcaacta ggtttaagaa cacttgcttt atatagtctt tattggatag tatacttatt
    11101 gatttatcat cacatttata taaaacttct atattttttt cactagctga atgttgagtt
    11161 atttcatata cctctttaaa tacatcatat actacaacat tttccataag aagagtatct
    11221 ttattttcta caaatgataa caacaatata tcatcaatca acctttttaa tctatctgat
    11281 tcgctttcta ttatttctaa gaatctattt ctagtattca catctatatt ttcatttgac
    11341 tttagtgttt caacaaaccc acttatagat gtaagtggtg ttttaagttc atgacttaca
    11401 tttgcaacaa aatcacttct catattttca agtttaactt ttttagtaat atcttctata
    11461 tttactatcg aacctattat tacgttgtta gtattctgaa gataaactgg gtctactttt
    11521 atcttatata cgatatcatc tttagtagta atattagcag tttcattatc tttagaacca
    11581 ataaattgaa gtatttgttt taatatttct tcttctttta ttacaacatt aatattatga
    11641 ccttcaacta attgatttgg ttcactcttt atcatttctt ttgcttcatc atttatcaga
    11701 agtatatttc catttacatc tatagctaaa attccatgag atatactctt taatatagac
    11761 cttaactgta aatgtttata ctgtacttct tctaaggttt tatccatatt ctttatcata
    11821 tgattaaaat ttctagctaa ttcgccaagt tctcctttgg ctgatatgtt aagcctagca
    11881 tgatattcat tattacttac tttttttgag atgtatataa actcttttaa atatttcctt
    11941 aatgttattg tgtatcttac agctaatata acaatggcta tggacaacat aacaataaaa
    12001 aaatatatac tatttaaatc ttccacaaga taacactcct aaaatataac ctttttaatc
    12061 tattttgtat cctacacctc ttatagtctc tatatatttt tccgatttat cttcatcttc
    12121 tatttttttt cttaaatatc ttatatgaac atctactgtt ctagtttctc catagtattc
    12181 atacccccat attttatcca aaagataatt tcttgaaagt acttttcctt tattttgaat
    12241 taataatttt aacaattcaa attccttaag agttaactct atcttttcag agcctttggt
    12301 aacttcatgt tttgataaat ctagttttaa attacctgta gtaagtatat tgttttcttc
    12361 acctaagcta gtcaaattat atctccttaa aacagaacta atcctagcta acaactcttt
    12421 tatactaaat ggcttagtta tatagtcatc agcacctatt tcaagccctt caaccttgtc
    12481 attttccata tttttagcag taagcattat aacaggaata ttttttaagt ccttatcact
    12541 tcttattttt ctcaaaactt ctattccact tatatttggt aacatccaat ctaaaagtat
    12601 taaatctggt ttaatttcct tagcctttat aaatccgtca aatccatcat atgaataact
    12661 aacttcgtaa tttgacacct ccaaattgaa ttttaatagc tcaactatat gcatttcatc
    12721 gtctataaca agcactttag tattcattaa aaagctcctc tctattttat ctgtagtacc
    12781 gttcctattc gttctgatgc cttattatgt tttctataag agtggaattt atctgctcta
    12841 caactagtgc ataaatttaa atttattata ttttcttttt taactccact acatctaagc
    12901 atatactcat taattttcca taaatctaaa tagtaacttc cctcttttat tatatagaac
    12961 ttttcatcat tatttgtaag aattgtgtta aatttttcta ctaaatcttc agacacttca
    13021 taacaacatg gtccaattga tggcccaatt atacatacta aatcttcatg aactgtttta
    13081 tagcattcag acattaattt tattgtttta tgacctatat tactaaaagt ccctctccaa
    13141 cctgcatgag ccagtcctat ggctttattt ttagcatcta taattgatat aggtacacag
    13201 tctgctgtaa acagtaaaag tggtactttt tccaagtttg ttataagtgc atctccatct
    13261 cttcttttcc ctatagtatc tttattgact atattaacta tatctgaatg tatttgagaa
    13321 ttactagtta gattttcaaa tgtcaaatca cattctttac atacttttaa catatcttct
    13381 ctattttttg catctatatt cttacatgtt ataactagat ttacagaatt taatttttca
    13441 tcaagttctt cacaattacc acttaaatta acatactctt taatatcttg tacatttttt
    13501 atatttatat aatctttcat catttgtctc ctttttctat cagtatactt ttaagttcat
    13561 ttacaaaagt atttatatct ttaaattgtc tatacacaga agcaaatctt acataggcaa
    13621 cctcatctaa ctctttcaat ttatccatta ccatctcacc gatttttttg gtttcaattt
    13681 cttctataaa acatttattt atttcatttt caatatgtac aacaatactt tcaatctcgt
    13741 caatagaaac aggtcttttt tcacatgctt ttagcaatcc atattttatc ttttctctat
    13801 caaaatattc tctactatta tcctttttaa tgaccattaa aggagttgtt tctattttct
    13861 catacgtagt aaacctcttt ttacatgatt cacactcacg tcttcttctg atagatttta
    13921 aatctgtatg tctagaatct attacctttg attctttata attacaataa ggacattgca
    13981 tctatattcc tcctttgttt aactatatct caaactcatc taagcttaat tctgaaccta
    14041 tatttactat tattgtatca caacctattt taagtatatc attccaaaat ataacttctt
    14101 catcccctcc tcttgaaaaa aaacttctac ttccatctcc aaaaatggta aatgagacta
    14161 ccttaccttc gtgaatatcc atgtctatgt ctattataaa ccccatcctt tttccatttt
    14221 ttacatttat aatttctttt tccattatat cagaaactct aatcatattg tactcaccct
    14281 ttctttaaaa ataaatatgt atcgaagtaa aaaaaaatga cctatatagg tcatttaaat
    14341 taaaataatg tttacagtcc atggattact tatacatatt ttctcatatt ctttaaagca
    14401 tttttttcaa ttcttgatac ttgtgcttga gatataccga tttcatcagc aacttctatc
    14461 tgagttcttc ctttgtaaaa tcttaaatct aataccaact tttctctact atttaatttc
    14521 ttaatagctt ctttaagtga tatttcttgc aaccaatttt catcagtatc ttttttatct
    14581 tgaacctgat ccataacgaa tattgcatct ccattgtctt gatatactgg gtcaaacaga
    14641 gatattgggt cttgtatggc atcaagtgcc attacaactg attctacttc taattctaat
    14701 tcctttgcta tttctgatac tgtaggttct ttagaatttg ttctaattaa tctttctcta
    14761 acttgtagtg ccttatatgc tatatctttt aatgacctac ttactctaat tgggttattg
    14821 tctcttagat accttctaat ttctcctata atcataggaa cagcataagt tgaaaacctt
    14881 acattttggc ttaaatcaaa gttatctatc gcttttataa gaccaataca acctatttga
    14941 aataaatcgt ctatattttc tcctctgttg ttaaactttt ttattacact caagactaat
    15001 cttaaattcc ctcttacaaa ctgttgtctg gcttcttcat caccattttt tatttgtaat
    15061 aaaagttcct tcatttgctt atttttaaga actggaagtt ctgatgtatt aacaccacat
    15121 atttcaacct tattaacttg catatatttt cagtcctctc tcaaaagtat tcatctattg
    15181 aaattattta catttccaat ttttttatac tttttaagaa gaattaattt agcattatac
    15241 aaattttttc atttcttttt gcaaccttga gattattttc ttttcaagtc tagatatgta
    15301 ggactgtgat attccaagca ttccagcaac ttctttttgt gttttttcaa tacctttatc
    15361 aataagacca aatcttaatt ccataatttg cttttctctg tcacttaatc tgtcaagtgc
    15421 cataaccaac aaatctctat ctatttcttc ttcaattatt ttgtatattt catcattttc
    15481 tgtacccaat acatcagata ataaaagttc atttccatct aaatctatat ttaaaggttc
    15541 atcaaatgat acttctgttt tctttttatt attttttctt aagtacatta atatttcatt
    15601 ttctatacat ctcgaagcat aagttgccaa ttttatattt ttattcagtt taaaagtatt
    15661 aactgctttt atcaaaccta tagttcctat tgaaatcaaa tcttctacat ctatacctgt
    15721 attttcaaat tttctcgata tgtagactac taatctaaga tttctttcta ttaaaattga
    15781 ctttacactt tcatctgttt ctagcttttg taaaagctcc atttcttctt ctggtttcaa
    15841 tggaggagga agtatatttg cccctcccat atagtatatt ccctttggct ttattatttt
    15901 taatttaata cctaacatag taataaagct tattattctt tctttcaatc gtaacattcc
    15961 ttgtccccct taaattttca cctaaaatcc aggcaatata tttggattca ttattgctcc
    16021 atattctcta tcattaaaat ttgaaatacc aagtattata tttcctatct tttgctcatc
    16081 tacttctata tagtcagctt ttaagcctat tattatgctt gtcttattac ttccagcatg
    16141 tttataaggt attattctaa ctctacctga agttttatca tctaaacttc ctataatttc
    16201 ttgtgctttc ttcatatcta tatcttcata attaatattt aatagttttt ctggaataaa
    16261 tccttctaac acactagact taactattat tacatcactt tgacttattg ggtcttttaa
    16321 caaattacca ctatctacta aagctttaca gcaaaaactt ttttctagta agtttatatt
    16381 tatagtcttt gtaagctcct taatgtactt aagcattttc aaatctttat aaatgtattt
    16441 taataattca caactcacat aagtacatgc aattaagaaa gatattttta agtgacttat
    16501 tcctgtaaaa taaattataa aataagtgct tccagaaata aaaatgttta ctagataaaa
    16561 aactaacgct acatgaacat aatctttttt gctcttactt ctaaatgaaa ttaaagttat
    16621 tatactcatg attattattt taaatggtac agtaaatagt atattcattg atggatacaa
    16681 ataagcaaca gaataggcac ctcctaaact agcccccaat aacttatttt ttttaggatt
    16741 atatctttta gtaagaatag aagtacaact tataataata taatttatta ctaggttttc
    16801 tataacatag tactcaatat acaccatgta tcccccctta aaacctttaa acatattata
    16861 agatagtatg tacaaaattt tatgtcactt ttgatggtcg aatccaaaac aaattctatt
    16921 taaaaagaac aatgatatat gtcttatact ttatgtcgtc aaaagatatt tttttaatac
    16981 caaacaaata gacttttttg tagaatccaa tatatctagg tagaagttat tcgctctact
    17041 tttggtatat ttacaaaaaa agactacata atttaataat atatcaagta aatcttttac
    17101 ttatataaaa tcaaattata tagtctgtaa attcaaatat aaattattgt gctagtaatc
    17161 catttgcata ttttgcagat tcatttagtg cttgttctgg agttatgtct ccagctatca
    17221 tcatttgaag ttgtttccat aaatattcat atatctctac tccatgaggt ccaactgtca
    17281 atggtctata catatgttta tctcttgtca ccatttcttc aaatcttggg tctccttggt
    17341 atttttcacc tttagcaact ggagctctag gtatttcttt gtggaaagct tccattgatt
    17401 ctttgcttag catatactca agaagtttaa atccaagttc tttattttta catgctgaca
    17461 ttaatgttaa atcatctact gaagcaaaac ttccaactgt cttatcttct aatgatgtta
    17521 catatcccca gtttaaatcc gggaatgatt tatcaaatgt acttgtagca gcagaggata
    17581 gcattatggt aaatgcagct tttcctggtc caaatacact ttcaatcatt tcattactat
    17641 cttttgataa agcatcttct ggaatatatt ttttcatatc atttataaag tttgcagctt
    17701 tcaagccttc tgcattatta aatttaactg attttaaatc atcattatat atatctccac
    17761 cagcttgcca taaatacgga taccagttcc agtttaagct tccaaatact ttagaacccc
    17821 aaccttgtgc aagaccccac tggtcgattt ttccatctcc atcagtatct tttgttgctt
    17881 tttcacagat tcttttaaaa tcatcccatg tttttggagg tttttctcct aatttttcta
    17941 gtatatcttt attgtagtac aatacgcctg gattagcagc ttctataggt aaaccataaa
    18001 taccacccat catttttgca tcatctaagt atagataatt atctgaagtt cctgattttt
    18061 ctaaataagg tgttaaatct tctaccgcac ccatttctat aaattgagga aacatctctg
    18121 catacatgta tcctatatct ggaccttcac ctgcacttat tgctgttgca tatttttctg
    18181 cgtagttatc ccatggtact atctgaaatt caatttcaca gttattttct ttttcaaatt
    18241 tttcaaatat cggcttccat gcatctgcgt catggtctgt taatggtgca gaccatacaa
    18301 ctaatttttc tttaccctct ttgcctttag aatcttcttt ttttgaacat cctgcaagaa
    18361 ttgatactga tagaatccct accataacta aagaaagaac cttttttagt ttaatcatgc
    18421 ttaatcccct ttcagacttt aatttgtctt tttcctcaaa tgtttacatt taattcagat
    18481 ttaatattaa caaataagat tactcttgtc taatcataaa aaactatact aactcctata
    18541 actatatttt ttttctatac ctattagatt taattcatac taaaaactta ctttttaaat
    18601 tttatcaata tttaaaaata tttttaagtc tagccacaaa ctaactatct aaattgaaca
    18661 aaataaaaag aagtctattg acttctttat aagcttacat attatcttct tcttcttagg
    18721 aatgtaggta tctccatgct ctcatcatca tcttcttcaa ttatacttgc ctttttagga
    18781 ggctctattt tttcctcagc aattacttct tcttcaattt cttttacagc ttgttttact
    18841 gttgtattta atgaagaacg tatgcttgat ttaggtttag tatctaaatc taaggcacca
    18901 ttttgtaatc cttcaaatcc tgtcgcgatt actgtaatca ttatttcatc acctaaatct
    18961 tcttttatag aagctccaaa tataacattt gcttctgggt cacaggattc cataactaaa
    19021 gttgatgcct cattaacttc aaataatcct aagttaggac cacctgtaac attaagaagt
    19081 actcctttag ctccttgtat tgatgtttct agaagtggag attgaatagc ttgtcttgca
    19141 gcatcaattg ctctagtttc accactagca ctacctatac ccatatgagc aagtcccttg
    19201 tctttcatta tagtagtaac atctgcaaag tccaagttta ttaacccttc aactgctata
    19261 agatctgaaa ttgattgtat accttgtttt aatacatcat ctgcaactgc aaaagcatct
    19321 aacattgatg tatttttttg tactatctgt aaaagtctat catttggtat cgttataaga
    19381 gtatctactt ttgatttaag ttctgctatt ccaccttcag cgttcttcat tcttatctta
    19441 ccttcaaatg caaaaggttt ggtaactaca cctactgtaa gtatccccat ttcttttgcc
    19501 aagcctgcaa ccactggagc agctcctgta ccagtaccac cgcccattcc ggctgttaca
    19561 aagaccatat cagctccttg aagtaacttt actatttcat ctttactctc ctcagcagct
    19621 cttttcccta cttctggatt tgctcctgct ccaagtcctc tagttaattt ttctcctatt
    19681 tgaactttat attcagcttt tgatgtatat aaagcttgtt tatctgtatt tactgatata
    19741 aattcaacgc cttttagttg agcttctacc attctattga cagcattgtt tccaccgcca
    19801 cctacaccta ttactttaat ttgtgcacat tcttctaatt ctacgtcaaa gtttagcatc
    19861 ataaaccccc ttctctcatg attcaaagtt atcgtatgta ttaattatat cattttcttc
    19921 ttttattttg ttaaccttta agtaacatat tccacaaaaa cgtaaatttt cctttttaaa
    19981 tttcttactt atattgtcaa atattactct ttaagtataa aataatttac gtatatatta
    20041 aatgctcttt aatttttacg ttttttgttc ataatattac taataatata tcgtctaatt
    20101 atagataagt tattaaacaa tctcatacca aaaactaata cagcaacata gtaaagagaa
    20161 actcctaact tttctcctat ataagtcaat aatatagcca atatagcatt acctatgaaa
    20221 ccagtaataa aaattacatt atcatagtta tcttcaaaat tggattttat cgctccaaaa
    20281 atagaatcca ttattgcaag aatagctact gacatatata tggaataatc catagaatat
    20341 gtaaatggaa tatagtaacc tactactaca ccaaatgcta atcctcctaa aacccataac
    20401 ataaatcttc acctctttat tttactttga aacacaacta cattacaatc ctttttatta
    20461 atttttttta ctttttcttt atttttttgt atttttcgtt ttattttgca aataaatcca
    20521 ctttatttcc attattttcc tgaactttta tattaattcc atatatatcc ttaagagatt
    20581 tagcatatga ttctggagag tttatagctg cactcaataa atctatatct cctatagctt
    20641 ttattataaa tggttgtcca tatgtagtat catttactgt aatagtagct cctgaacatt
    20701 ttatcttact tgttgctagt actctttctc catttataga aattgctctt gctccagcct
    20761 tttttaaatc atttaaaacc ctcaaaatat ctatatcatg tataatcaaa tcgtttggat
    20821 tctgtccgtc ttttaactct ctctcactat ccctcatagt aatagttatg cctggccctg
    20881 taacagttga ataaccactt aactctttaa cttcttcaag ctctttttgc ataagtgttt
    20941 ggatagattc atttccattc ttttttgctt ctttatattc attcaaagtt tttttgttct
    21001 ttttaagagt ttcttttaaa ttttttattt ctttttttgt attttctacc tcactttcaa
    21061 tactttcagt ttgacctaat gttatgtata ctctatttgg gtctagtgat tttatataag
    21121 ttgatatgaa aacgcctaat atgaatgctc ctaatataac tattttcttt ttcatactat
    21181 tatttcccct ctgctttgtt tatatcacta tgaccattta tttctatatt gtcttctttg
    21241 gtcactttta ctttggcatt ataatatgtt tctatttccc agacaatacc atactttata
    21301 ttaagtgctg aagccaatgt atcagaatca ccaattgctt ttattataat tggttctttt
    21361 atgacagtgt cattcatata aagcttgtct tcttttaaat acatatatgt attagaaact
    21421 attctctggt cgtttataga tatggcactt gcttgtgctg aatttaattt atttatcatt
    21481 gataataata aatcataatt atatagtata ctatcatttt tatcagagtt tcctaagtct
    21541 tttgtagaat ttatctcaat ttttatgcct gttccttcaa catctgtata tccagctaat
    21601 gtttcatatt tttctatttc tgacttaagt atgtcttgtt ctccttcatt gtttttgcct
    21661 ctataagtct ctatatcttt cttcacacta ttaacttctg attgtagatt ctctttttct
    21721 ttttttaaag atttaagttc ttctgtaagt tgctctcctc ttttaaaagt tgtcatccct
    21781 ctattttcta tattaataat tttaagttgt aaactaacaa gaacacccaa tacaatactt
    21841 cctatgatta tacttttatt tataaatctt tttttcaata tatcacttcc ttacaattgt
    21901 ctatccttct ggactataca ctgccaactt tccatagtta agatttatag tccctccatt
    21961 agtattttta ttttgcaggt caatcagaat tttacttacc ctagatatat tataatctaa
    22021 gttatcatca ttagaaagta agatttttat attacttctt gtaagcattt ctatattgct
    22081 ttcttttttt aagtttacat aatttatttt tctataaatt ccttcttctt ttaaataatt
    22141 taaagtttta aatacattct ccttagtttt ataattttta aattttatag atttattatc
    22201 atcaatgcta taatctacat ctactatcaa gtctttttta ctttcattag aacctcttag
    22261 ttcttccaat aaatttcctt ttttatctat ataacaataa ttatcttcat cttttaatac
    22321 tgcaaatatt tctttttctt ttaaacttat aattatctta ttgggaagtt ttctttttat
    22381 ttccacattt tctatatatg gattttttat aagtttattt ttcatatctt taaaattgta
    22441 tgcaaaaata ttttcattta gatttacatt tagttctttc attatatttg atttggtcac
    22501 cctcttattt ccaattacat ctattttctt tacatcaaat aaatcacttt ttacaattat
    22561 acatatacaa aaagataaca taagtataaa aactaaaact atcatgacat tattcgtatt
    22621 taactttctt ctttttttca aatataatca acccctttgg ttaataaata cacctttaga
    22681 gcttgttttt cctacaaaaa aattataata tttaaagtca aatttttgca ttttattaca
    22741 taacatccaa ataaggtatt aaattttata aataaatcaa tatttcatca aaataaacat
    22801 agcatactac atactattta tatatttaaa tctgtataca tacaaataaa cccctaattt
    22861 ataataattt agaaaatcta atatattata aattaggggt aaatatttac ttatcttttc
    22921 tttattccta ttaccttagc ttgaccttct atacttggag tcgtttcttt ttttatttct
    22981 tttacttcct taataacttt ttcttttttt gtttttttag aagttgactt ttgtgttgaa
    23041 ttgtatactt tcattatctc atcatatata aggtctatgg cttcaggctt acctatagtt
    23101 ttacttgcat ttgccatatc aaccaaaagt tctctatctc ctaaaagttt aaatactgct
    23161 gtatttaggc tctctggagt tagatttttt tctaaaatag caattcctgc cccttgtttt
    23221 tctatactct tggcattata ctcttgatga ttttcagcag tataagcttt aggtattatt
    23281 atagatggct ttccaagagc cgttatctca gctaatgaaa ttgctcctgc gctacctata
    23341 accaaatcac ttgctgcaag tgcatttgcc atatcttcta agtaaggcac aactttttgg
    23401 tatggtttaa gatttatatc gctaatactt cccataaatt catcataata gcttttgcct
    23461 gtagcaaata taaaagctat atcttcattt accatattct ttattacaag tctcattgca
    23521 tcatttattt ttcttgaacc tccactacca ccatagcaaa gtaccattct tttttcgtca
    23581 cttattgata gattctttct tgcaatattt tttcttgata ataaaatttc ttttctaact
    23641 ggatttcctg taaaaactaa tttatcttct gctgcctctg gaaatctttt atgcgaatct
    23701 tcaaaacttg taagtacctt tgtaactgtt ttagataaaa tcttattagt tacaccagga
    23761 aaagaatttt gctcatgtat tatggctggt attttcccca tagatgcatt aaataaaaca
    23821 ggtccactaa catatccacc agttcctata actatatctg gtttaaattt cttaacaatc
    23881 tttcttgatt gctccaaacc tttaaaaagc ttaaaaactc tttttacatt atcgaaatct
    23941 atttttcgct taaagccttg aactgttaca gtctttaatt caaatccata cttaggaact
    24001 atttctgact ctattccctt ttccgtccca acaaaaatta tctcagcgtc tggatgctca
    24061 tctcttattt tgtttgcaat agctatagct ggatacacgt gccctccagt tccaccgcct
    24121 gataataaaa ctttcatcaa atcatcatct cctgttaatt aattttgaca tgctttgaga
    24181 tgtttaacac tattcctaca gctcccataa agatggtgag tgaagttcct ccataactta
    24241 taaatggtag tgctacacct gtagctggca ttgatgatgt cgcaactgct atatttaaag
    24301 ctgcttgtat tccaatttgt gcacctatac ctataacaac catacaagca aatacattag
    24361 aagtcttcaa agctattctt acacatctgt agactaaaac tacaaatagc ataataacta
    24421 ttatacaacc aatcaatcct aactcttctc ctattatagc aaatataaag tcattttgtg
    24481 gctctggtat ataaaaatac ttttgtttac tctttcctaa gcctagacca aacaatccac
    24541 ctgaacctag tgcatataga ccttgtatta cctgataccc cttaccaaga gggtcttgaa
    24601 aagggtctaa aaatgatgta acacgactaa gtctataggg ttcagcaatt actaatgcag
    24661 caaataaagc agccccactc cctatcatag caaatacaat tttcatatcc attccagcaa
    24721 caaatatcat tacaaaagta actagaatta cagtacctgc tgttgacaaa tttggctgaa
    24781 gtattatcaa cgcaaaaaat atccctggaa ctacgagaag tggtacaaca ccttttgtca
    24841 aagattttat tttatcataa tttttttcaa ttaattttgc agttaatatt attgtagcaa
    24901 actttgcgat ttctgcaggt tgaaatgtta aagctcctat tcccaaccat ctttttgctc
    24961 cgtttgcttc tatccctaaa ggggtcaaaa ctaacaataa taatactact gctatagctc
    25021 caattgctgt agcatttttc ttccaaaaac tataatctat tcttgatgta ataatcataa
    25081 ctataaatcc aagtacagca tatattacat tcttttttag aaaatagtac gcatcattgt
    25141 gtttaaatga agattgtata aaacttgcac taaacaccat aactattcct acaaatacta
    25201 aaagcatagt agtgtaaaac actactccat caaattcagt cttcatcctt atgtcaattt
    25261 gttttttcaa attttcttta ggcatccaac taatctacct ccattcttcc atttaagtaa
    25321 gtttagaggt aagagattat tttaaattgt ttacattatc tttgaagtct tttcctctta
    25381 cttcaaaact ttcatacata tcccaacttg cacaagctgg tgaaagaagc actatatctc
    25441 cactttttga aatttcataa gaagtcttaa ctgcttcttc catatctttt actatatgta
    25501 tgtcattaaa tcctttattt ttagcacaat tttctatatt tgatgcagtt tctcctaata
    25561 agaccaatga tttaacatat gactttgcag tttcaagaag ttcatcaaaa ttacttccct
    25621 tatccatccc ccctgctata agtattatag gtctatcata agactgtata gcttttatag
    25681 tggaatctgg attagttccc tttgaatcat ttacatatat tacattgtct aaatttctta
    25741 caaactcttg tctgtgttcc actcctttaa atgtcataag tacatccttt ataacttcta
    25801 aatcaatttt gcagacatat gctattgcta tagctgccat acaattttct aagttatgac
    25861 ctccaggtaa acttacatca tctttattta gaaatttgat tttatcatct atatctatta
    25921 ttatgtcatt attttctaaa tatactcctc catttacttt tttagttctt gaaaagtaaa
    25981 ttactttggc attgcattta tcagctaaag attttactat ctcatcatca tagtttaata
    26041 cacagaaatc ctcaacagtt tggttcataa atatatttgc ctttgcttct atgtattttt
    26101 ccatagtatg gtgtctattt aaatggtcct ctgtaatatt taatattgcg cttactttag
    26161 gtctaaattc atctatactc tcaagctgaa aactgcttag ttcagttact aacacagact
    26221 cttcacttga agtttctata gtatctatta caggatttcc tatattacca actacatatg
    26281 tatctcgttt tgctcttgaa aaaatttctc ctaccaaact agttgtagtt gtctttccat
    26341 ttgtacctgt aatccctata aaaattggat tattcgatag tttaaaagct aattctacct
    26401 caccaattat atacttgcct gaatttttta atttcaatat gaatggtagg tctagtggta
    26461 cacctggaga cactacaacc atatctatat catctacatc ttctgggtga tgtcctaata
    26521 tgtattttat gtcatttata gatttcaatt catcaagtat atttctcaat ttattttcat
    26581 cttttatatc atttacgatt atacttgcac ccaatttatc taaatgttta attgtagata
    26641 taccagtttt tgcaagtcct actaataaaa ctttttttcc ttttaagtcc atttttcatt
    26701 ccccctagcc ttaaaatact gctattatac ttatccaagc taaaacaact gttataatcc
    26761 aaaaaataaa tacaactctt gtttctggcc aaccacactg ttcaaagtgg tggtgtattg
    26821 gagccatctt aaagaatctc tttcttgttg ccttaaaata tccaacttga atcaaaactg
    26881 atagagcctc cgcaaaataa attcctccaa ttataggtat tataagcact gagttagtta
    26941 gaactgaaaa tgctacaact gcacctccta atgccattga acctgtgtcg cccataaaca
    27001 ctctagccgg atatgaatta aatcctaaga aacctaaaca tgctccaaca gttgctgctg
    27061 ctaaaactgc gacttctgta tttccagcaa tagaacttgc aaatagcata aagaatacag
    27121 aaactattaa tgttactcct gatgctaatc catctaatcc atcagtcaag tttacagcat
    27181 ttactattgc tacgattata aacatcatta ttggtatata taatattcca acatttatca
    27241 caaaatctgt aaatggtatc ataagttgtg aagcacttga tgatgatgta tactgataaa
    27301 aagcaacata aaaagataat gcaacttgaa ggattatttt ttgatatgct tttagtccaa
    27361 gtgatctttt tagctttatt tttataaaat catctaaaaa gccaataaaa ccaaaacctg
    27421 caatacatat aagacctact gccatatcat gactaacttt tactcttgta agtccagtta
    27481 ttagaattgc aacaatcatt ataattccac ccatagtagg tgttccgttt ttagctagat
    27541 gtgtttgggg tccatcatct ctaactgtct gaccaaattt aaattttcta agcattggta
    27601 taaatattgg tcctattata attactatta aaaacgcaat caatgcggtg tacgtaagct
    27661 ctgttattcc taacatcata aaactcctct cccttgtcaa ttaactcatt agtttaagct
    27721 tatttattta gatattcgac tattttttct aatctcatgc ctcttgagcc ttttactaaa
    27781 atagtgtctc ctgtttttac taattcattt aacttgttaa acacatcttc tctattctca
    27841 aagtgatata tattggctga attgaatcct aattgttttg cttcttctcc aataaacact
    27901 gaattttcac ctatagttat tataatatca gtattattca tagatgattt tccaactaat
    27961 ctatgaccat actctgaaat ttcacccatt tctagtatat ctccaagtat cgctaccctt
    28021 ctacctttat atcttccaag tatctttaaa gcagcatcca tagaatctgg actagcatta
    28081 tatgaatcat taattattgt taatttttca ttttttatta tgtctaatct catttttgtc
    28141 cctttaaaat tttttaaacc tttttttata taatctaaag aaatatttag acacaaacca
    28201 actaatatag cagacattgc attatatata ttatgttctc ctattgttgg tataaatatt
    28261 tcctcttttt tcccatttat tacacatata aatgttaaac tatcctcttc catagtataa
    28321 ctttcacaat aaacatcatt gtttttatta aagccaaatg tttttaactt atatacatgt
    28381 tctttttctt ttaatgtaga taaaaactta tcatctccat ttacaattaa agtatttgtt
    28441 tcatcaaagt ttgaagtaat ctccatcttt gctttcaaaa taccctcttg tgaacctagt
    28501 ttttctacat gtgataatcc tatattcgat atgactgcaa ttttaggatt tactatatcc
    28561 actaaatatt ttatttcatt gaatccagac atacccatct ctattacagc acactcatgt
    28621 tctttattta gattaaataa agtcaatgga actccaaaat gattatttaa gtttttttca
    28681 ttttttagta tatttaactt tgcagatatg gcagagtaaa tcatatctct agtagtagtt
    28741 tttcccacac tacctgtaac acctataaat ggaatatcaa atttttcttt atagtatttt
    28801 gaaatatctc ctagagccaa agatgtatct tttacctcta ttaaatttat atccgaactt
    28861 tctaatttta ttccattact ttcatttttt ataaaagttt tacatccact atcatatgca
    28921 gacttcataa atgtatgccc atctaaattt tctccaacta tggctacaaa tgcattttgg
    28981 gcatttgcct ccctgctatc tataactata tcacttacac agtcattata atctccatat
    29041 attaacttcc cattagttgc aagtactaac tcttttattg ttaagcactc cattattaaa
    29101 ctcctccttt aaaaccttag ctatattctc ttagcaacaa aaggcaatgt ataccgttat
    29161 atatacattg cctacgataa ctcttaactt tataattata tactatattt ctgttttttt
    29221 gaagatgttt atgggtatat gttaattatc tgttactttt tagttttcaa aatatagggt
    29281 tatacttgag tcttcattaa cttttactcc aggttttggg aatatatcct ttacctttgt
    29341 atcttctgga agttctatat ctgcatctaa gtctggtttt aacttcgcat cctccaatgc
    29401 ctttacagca tctcctattt ttagatttct tacatctggc actttaactt gcttcttttc
    29461 atactcagct ttttcttctt ctttatatac tggtttaact cctaaatatt ttaaagagtc
    29521 attcattatt tctttcacta ttggtcctgc tgtagtacta ccaaatgcac ttactcctgt
    29581 tggttcgtca acaatagcaa gaactactat ttgtgggtca tcacaaggtg ctattccaac
    29641 aaatgaacat atatattttc ctggagcata cttaccatct ataacttttt gagctgtacc
    29701 tgttttacca ccaagtctat aacctggtat atacgctatc tttcctccac cttcagttac
    29761 aactgattct gcaatttcta acattttttt agatgtttct ttagatataa cacttcttac
    29821 tttttttggt tttactgttt cagttatatt tcctttattg tcagtataag attttaccac
    29881 tcttggttgc attaaatctc caccattagc tattgatgat atagctgtta taagttgtat
    29941 tggagttact gatatagatt gaccaaatga tattgtagcc aattcaactg gtcctacatt
    30001 tttttcgtta tacagtattc ccttagcttc tcctggtaaa tctatacctg tcttatccat
    30061 aagaccaaat gattctatat aatcatacat ttttccaaca cctaatctgc ttcctaactc
    30121 aacaaatact gggttgcaag agttttgtac tgcttgttta aattcttgtg ttccatgagg
    30181 tctgtaatgt ctccaacatt taatctttct tcctccaaca gttacactac ctgtacatgt
    30241 gaatttttct ccatctttaa ttactccttc ttcaagagca ctggaagatg taataagctt
    30301 aaatgtagaa cctggttcat atgtatcact tactgctgga tttctccaca tttgataata
    30361 gccttttatt ttatctttat cattatattt ttcaagttct tcttgataat atggatatat
    30421 tggcgttctt gagtcattag ggtcataatc tggttttgat gctaaagcaa gtatatcacc
    30481 tgttttaggg ttcatagcta ttgcagtaac tttttttgca ttatttaact cataagcttt
    30541 ctgtactgct ttttcagtat agtgttgaat tacttcatct atagacaaca caagaccatt
    30601 accttgtaca ggctcataat atttttccat tccttgtggt atttctcttc ctgaagcatc
    30661 agtacttact ataagttttc caggctttcc ttttaacttt ttatcatatt gcatttctac
    30721 accagatata cctgttgcat ctgaagaagt atgtcctaac acatatggtg caaaattccc
    30781 atatgggtaa tatctttgat tatcttctgc cacccatatt ccacttaatt tagcatctct
    30841 tatttgactt gccttatcat catctatcca tctttttact tttacaagag ccatattttt
    30901 tttgcttatt aaagcgtaaa tatctttata atcttcatcc aaaatctcag ctaccttttc
    30961 ggctgcttct tttttatctt ctacttccac aggtttacac caaacagtat acttagttac
    31021 acttactgct aactctttca tatttctatc atatatagtt cctcttttgg gttctattgg
    31081 tatatctctt gtttgttgtt ctagtgcttt tgtactcaac caatttccct tcataagttg
    31141 tagataccca gtcctaataa caaggcaaaa aaacaatgca catgctagaa taagaacgag
    31201 taccagcctt ttcttactta tcctctttac ttttctcaaa tctattcccc ctagttatcc
    31261 acatctatat actttatttg tccatctttt ggatagtcca tattgagcaa ttccttagcc
    31321 tttagctcaa tttcttttat tgatgttttt tcagtttctt ttgcctttag ttcctctaat
    31381 actatctctt tttttcttaa atctttttgt aaataataga tatcgtactt catttccgag
    31441 atttttgaat aaccacatag attcaatatt attactgaaa aagttgcaat cagtaaaatg
    31501 catttagctc ctattttttt cttatttctc tttttataat tattcttttt tttattttta
    31561 tattcattta gattatgtat tttataattt tttttcaaat tctcaccccg atagtatata
    31621 gtttaaattt tcctctatac tatatttgtt attaatcata tttcttcctc taaaatttca
    31681 tcgctaaatt aatacactat tacacaatct aattgtattt ttttctatac acaacaaaaa
    31741 cctataaatt cacttatact tttatatgaa tctataggtt agatattaat ttctatttat
    31801 ttttatcact tctagtattt ttagataatt tcttccataa atataatatg taactaacta
    31861 tctactctct gattcctaag ttctccatta tattacttaa taccatctat atctatattt
    31921 ttatatctaa attttcatta tatatctcaa taacaaagaa catttcttat agtgttaatt
    31981 gttttttaat tgctttacca gtgcaacaaa atatataata ctttttaatt aattataata
    32041 atcttatata taagattatt ctttagcctt ataactactt atattttttc tgctactctt
    32101 agcttagcac ttcttgaacg tggatttact tctatttctt cctcactagg taatataggt
    32161 tttctagtta ttattttcac ctcagattct ttatcacatt ggcatatagg taaatgctgt
    32221 ggacatatac aatctgatgc taaatgctta aatgcatttt tcactattct atcttctaat
    32281 gaatggaatg ttattataca tattcttcca ccttcattca ttatagaaga agcatcattt
    32341 atcatctttg taattactcc aagttcatta tttacttcta ttcgtattgc ctgaaaagtt
    32401 cttttagctg ggtgaggtcc atctattctg gctttcttag ggatagcttt tttaattaca
    32461 tctactaatt ctcctgtagt ttctattggt ttgtttgctc tttcttcaac tataaattta
    32521 gctatacgct tagcccagtt atcttctcca taatctttta ttatcttagt taattcatct
    32581 tctgtataag tatttacaac atcatatgca gaaaactcac acctaacatc cattctcata
    32641 tcaagtggtg catcttgcat ataagaaaat cctctatcag cttcatcaag ttgatgagat
    32701 gaaactccta aatcagctaa tacaccatca attttgtaaa ctcctatttt ttctaattcc
    32761 tcttttatat tttcaaaatt gctgtgaaca aacttcactc tactttcata ttcactcaat
    32821 ctctcttttg ctgtgcttat agcattttta tcttggtcaa accctataaa taatccctta
    32881 tctgacagtt tttttactat ttctttagaa tgtcctgctc ctcccatagt acagtcaaca
    32941 tacacgccat ctggctttat atttaaattc tctatacact catttaaaag aactgataca
    33001 tgatgaaatt ccatagttac ctcccttaaa ttcacttttt aaactattct tcactaatat
    33061 tatttttatt tttctttgat aaataaacat gtgcaattat gccaccaata actccaatta
    33121 gacttataac ttgtgccatt ctaagtggtc ctaacattaa actatctgtt cttaatccct
    33181 ctataaaaaa tcttcctatt gaatataaag ttatataagt aactataact tgaccttcat
    33241 attttttatt ttttctaaat agtaataaaa ctataaatat tccaaaatcc catatagact
    33301 catataaaaa agttggatgt acctttactc catcaaccat tattccccaa ggtaaattgg
    33361 tagcacctcc atgtgcttct ccattaataa aatttcccca tcttccaata gcttgtgcta
    33421 aaggcattcc aagtataact gtatcagcca tttttaagaa gttaatattt ttaatcttag
    33481 tgtatatata accagctaat attccaccaa ttaaccctcc atgtatagca aggccaccac
    33541 ctctaaaatt aaaaatttga gacatatttt gagcatagta actccaatta aatacaacat
    33601 aatagattct tgcacagatt agtccaactg gaattgctat tatagctata tttaatacat
    33661 catcttcttt aattcccact ctttttgctt cttttattgc tattaaagtt cctaaaatca
    33721 ttccacatgc cataagtatt ccataccaca tgatgtctat gccaaacagt gtaaatgcta
    33781 ctctatccat aacgtcacct ctttgtatta attgaaaata tttccaaagc aatatgctat
    33841 aattacaatt aattgtaatt atactaaatt atgataacac attaatgtta attctacaaa
    33901 aaatattaaa atttattcta caactcccta cactatatta tatctaatta ctataaaatt
    33961 ttttaaactg aatctctcat gactaaagtt acaagtgtac atagatattt tttaatcaaa
    34021 aagattgatg catatataca tcaacctctt ctatttctta tctatagctc ttaaattttt
    34081 acctcaattt atataaatat attttataaa tttgttttac caaaataaac atttaaaaac
    34141 ataaactaat acttaattag ttattcttat tatgattaag tttctattta ggctctacaa
    34201 tagaaataac acaatactct tctttaaaat gctcttttaa tctttcttca acttcctcaa
    34261 aagtaacatc tttaataata tctaaataat ccaatagatt tatatcctta aaaacataag
    34321 atataaaact attacctata aagtttatag aatcaaagca cttaataaat gaccctattt
    34381 ttttcttttt agttctctca aactcttctt ttgataatcc ttcttttttt gatttttcta
    34441 tataatcaag aattatttct tttacttttt taggctcttt agaatctcca gctattatgc
    34501 taaaagcata atccacttga gacgaaaatc cagctccaaa attttcattt attaaacctt
    34561 gcatatacaa atcttcataa agcttacttc cttttttaaa taacatccct acaagtatat
    34621 cagttacaat ctccttacgt aaaagttctt tcccttttaa accaacatta ctatctttaa
    34681 aacctatgtt gaacataggc atagatatag ggaatttttc tataacctct ttttctttta
    34741 caccttctgg ttcttctgga taaaatcttt ctatactctt agagagtttg tctactttgt
    34801 aattgtttga ctttttagtt acatctataa ctttttctac atctaagtca ccaacaacaa
    34861 ataaagccat atttcctgga ttatagaaag tattataaca tttatatagc tcttccttag
    34921 ttatcttata tatactatca acagtacctg ctatatcaat tctagcagga taatttatat
    34981 acattgcctt caagcaatta aagtatacat tccaatcagg gtcatcattg tacattttta
    35041 tctcttgtgc tattatacct ttttcctttt caacattttc atctgtaaaa taaggtgttt
    35101 gaacatagtc aattagatgt tctaaactct cataaaaatt ttcagtagca gaaaataagt
    35161 aggctgtcat tgtaaaatta gtaaatgcat ttgcatttac tcctaatttt gaaaatttat
    35221 caaatgcatc tcctccatca ggttgctcaa acatcttatg ctctagaaaa tgtgcaatac
    35281 cttcattcac tctaattttc ttatcttctc caattggtac aaattctaag tcatttgaac
    35341 cataattagt agccaaaata gcatactttt tcataaaacc ccttttaggc ataaaatata
    35401 catctaatcc attttgcaat ttctcataat aaacttcttc tttcaatata tcattaacta
    35461 ttttttccat agacatctcc tccattaatt tctcaagaag tatacagtat caagctctat
    35521 attttttata gcttcaacta tatctttttt tgtaaccttt tctatactag atattatatc
    35581 ttgtacttca gaatttgtct ttgccataga ttgagagaaa acaaaatcag acataccacc
    35641 tatattatca gttatagatt tcatagaatt aactaaagca agtttactgt tttctaattc
    35701 ttcatcagat atatcacctg cttttacact ttctacttgt ttttttatca attctacagc
    35761 tttatcataa tctttagttt ctataccaga tgatataaac atgatacttt tatacttctc
    35821 tattgaagag aatatatagt aacataaact ttctttttct cttacattaa caaataattt
    35881 agagtgtgga ccaccaccta agacattgct cccaactact aaagcataat atttatctat
    35941 gtctgcataa tccacatttg ctctataacc cataactaac ttaccttgag ttatttccat
    36001 ttgttcatct ataactttta cctcatccac attttttata aaatctgctc ttggtatatt
    36061 tataatttct tctctttcaa attttagatt tttacttatt atatctacaa ctttatcttc
    36121 atcaaaatta ccttctacaa ctatatctat aggtgatgtt ttaagtatgt ttttataatg
    36181 ttcatataac tctcttgaag ttatactatc tatttcatct tcataaccaa attcacttac
    36241 actatatctc tctcctttac acatagcctc aatacattta tcttgcgcat attccttttt
    36301 atcatttatt attgattgta tcttttctct taaattctct ttttcaatat ttaaatattc
    36361 ttctttaaac ccaccatcta caacaagcgt attatttatt acttcattaa aaaattctac
    36421 tacttcttca aatatactct catctaaata cttttcactt atgttaatta ctttaaaact
    36481 caatacttga cgttctccac gttttacagc atcagctccc atagatgaac catataaatc
    36541 atctaattta ttggatattg ctctagcact aggatattta gcgcttccac ttgctattat
    36601 tgatggtatt agagcatttt ttgtagtttc tttttcatcc aatagtctct gaacatataa
    36661 acttataaga ttcgatttaa acttttctgt ttttatcaaa gtaagattaa tattattacc
    36721 taaatttatt atttttttag tattaaccat atgtacctcc tatttttatt taaacatcaa
    36781 ttttaaaatt gaaatttatt tattttttaa ttacatatac tc
//
LOCUS       DACXMZ010000012        34742 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.012, whole genome shotgun sequence.
ACCESSION   DACXMZ010000012 DACXMZ010000000
VERSION     DACXMZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.012"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            136..1329
                     /gene="nifV"
                     /locus_tag="JD016_02350"
     CDS             136..1329
                     /gene="nifV"
                     /locus_tag="JD016_02350"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HAU4998470.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1354..3279
                     /locus_tag="JD016_02355"
     CDS             1354..3279
                     /locus_tag="JD016_02355"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HAU4998471.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3301..4296
                     /locus_tag="JD016_02360"
     CDS             3301..4296
                     /locus_tag="JD016_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HAU4998472.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4646..5095
                     /locus_tag="JD016_02365"
     CDS             4646..5095
                     /locus_tag="JD016_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAU4998473.1"
                     /translation="MIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKGQFTFLTRI
                     CENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLTPTDKSLEV
                     YELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5129..5623
                     /locus_tag="JD016_02370"
     CDS             5129..5623
                     /locus_tag="JD016_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU4998474.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNQNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5720..6241
                     /locus_tag="JD016_02375"
     CDS             5720..6241
                     /locus_tag="JD016_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAU4998475.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            6430..6741
                     /locus_tag="JD016_02380"
     CDS             6430..6741
                     /locus_tag="JD016_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAU4998476.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6769..7455
                     /locus_tag="JD016_02385"
     CDS             6769..7455
                     /locus_tag="JD016_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAU4998477.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKGKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            7687..8571
                     /locus_tag="JD016_02390"
     CDS             7687..8571
                     /locus_tag="JD016_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAU4998478.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            8670..9845
                     /locus_tag="JD016_02395"
     CDS             8670..9845
                     /locus_tag="JD016_02395"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HAU4998479.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            9973..10827
                     /locus_tag="JD016_02400"
     CDS             9973..10827
                     /locus_tag="JD016_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998480.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFYEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            10871..12106
                     /locus_tag="JD016_02405"
     CDS             10871..12106
                     /locus_tag="JD016_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAU4998481.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            12207..13148
                     /locus_tag="JD016_02410"
     CDS             12207..13148
                     /locus_tag="JD016_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HAU4998482.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(13367..13720)
                     /locus_tag="JD016_02415"
     CDS             complement(13367..13720)
                     /locus_tag="JD016_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAU4998483.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDIIKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            14007..14927
                     /locus_tag="JD016_02420"
     CDS             14007..14927
                     /locus_tag="JD016_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HAU4998484.1"
                     /translation="MIIMNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVF
                     TMYNPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVY
                     NRDIVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREE
                     SPKFFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIM
                     SGGFNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYI
                     PTNSKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            15188..15622
                     /locus_tag="JD016_02425"
     CDS             15188..15622
                     /locus_tag="JD016_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998485.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            15645..17180
                     /locus_tag="JD016_02430"
     CDS             15645..17180
                     /locus_tag="JD016_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998486.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDVILVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            17264..18631
                     /locus_tag="JD016_02435"
     CDS             17264..18631
                     /locus_tag="JD016_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAU4998487.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            18666..18890
                     /locus_tag="JD016_02440"
     CDS             18666..18890
                     /locus_tag="JD016_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HAU4998488.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(19244..19642)
                     /locus_tag="JD016_02445"
     CDS             complement(19244..19642)
                     /locus_tag="JD016_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HAU4998489.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(19645..20079)
                     /locus_tag="JD016_02450"
     CDS             complement(19645..20079)
                     /locus_tag="JD016_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAU4998490.1"
                     /translation="MKSNYDCFRIAMLLKELYSKTMYTVEENFKENGLTHQQIIVIKL
                     IAHNQELTISQLCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFE
                     FATNFKIKMQESFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            20406..21392
                     /locus_tag="JD016_02455"
     CDS             20406..21392
                     /locus_tag="JD016_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4998491.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            21376..22287
                     /locus_tag="JD016_02460"
     CDS             21376..22287
                     /locus_tag="JD016_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4998492.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            22400..23968
                     /locus_tag="JD016_02465"
     CDS             22400..23968
                     /locus_tag="JD016_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAU4998493.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            24043..25068
                     /locus_tag="JD016_02470"
     CDS             24043..25068
                     /locus_tag="JD016_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4998494.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            25061..26005
                     /locus_tag="JD016_02475"
     CDS             25061..26005
                     /locus_tag="JD016_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4998495.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            26364..29021
                     /locus_tag="JD016_02480"
     CDS             26364..29021
                     /locus_tag="JD016_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU4998496.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            29236..29865
                     /locus_tag="JD016_02485"
     CDS             29236..29865
                     /locus_tag="JD016_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998497.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            29980..30306
                     /locus_tag="JD016_02490"
     CDS             29980..30306
                     /locus_tag="JD016_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU4998498.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            30307..31611
                     /locus_tag="JD016_02495"
     CDS             30307..31611
                     /locus_tag="JD016_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAU4998499.1"
                     /translation="MGGIKMEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPII
                     IVGSLSMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKK
                     YNLNHVEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDK
                     NLVIRFPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAI
                     SSVDNVFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYV
                     FTIGFYYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPI
                     VMNPYMFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIV
                     LVFASIILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            31635..31964
                     /locus_tag="JD016_02500"
     CDS             31635..31964
                     /locus_tag="JD016_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAU4998500.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            32112..33440
                     /locus_tag="JD016_02505"
     CDS             32112..33440
                     /locus_tag="JD016_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HAU4998501.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKTGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            33763..34617
                     /locus_tag="JD016_02510"
     CDS             33763..34617
                     /locus_tag="JD016_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HAU4998502.1"
                     /translation="MKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKEVP
                     SKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDIEE
                     VEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCDKI
                     IKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLNTI
                     KEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEEEE
                     EKYRRIFK"
BASE COUNT        13869 a         3548 c         6335 g        10990 t
ORIGIN      
        1 gaaaagctaa aaaaataaat tgcaaatgaa attaaaatga ataaaaaata aaaaaataat
       61 cataagttag aggtatcaat gaaaaataag acttaatgtt tttaaaatta aaagtatact
      121 aaggggggca ttaatatgtg cgttatatca aaagatagag caaaggaaat aaaaatagta
      181 gatactactt taagagatgg agagcaaaca gctggggttg tatttgcaaa tagagaaaaa
      241 ataatgattg ctgaaatgtt aagtgaccta ggtgttgacc aaattgaagt tggtatacct
      301 actatgggtg gagatgaaaa aaatgtaata aagcacatat gtagtagaaa tttaaaatca
      361 gatataatgg cttggaatag agcagtaata aaagatgttg aagaatctat ttcttgtggt
      421 gttgatgcag tagctatatc aatatcagta tctgacattc atatagaaaa taaattgaga
      481 acttctagag gctgggtttt agaaaatatg gctaaaacag ttgaatttgc aaagaaaaat
      541 ggattatatg tttcagtaaa tggagaagat gcttcaagag cagatataga cttcttaact
      601 gaatttataa atgttggaaa acaagcaggg gcagatagat ttagatactg tgatacagta
      661 ggagttatga atcctttttc tataaagaat gcaatagaaa ctctttatga aagaactaat
      721 tttgatatag aaatgcatac acataatgac tttgggatgg caactgcaaa tgcacttgca
      781 ggaatagcag caggagcaaa ttatgttggt gttacagtaa atggattagg tgaaagagca
      841 ggaaatgcag cattagaaga agttttaatg gcacttaagt gtgtttataa atgtgattta
      901 aataatatag atactagaaa atttagagga atatgtgagt atgtagcaca ggcatcagga
      961 agaatacttc ctacatggaa accagtagtt ggggataata tgtttataca tgaatctggt
     1021 atacatgcag atggagcttt aaaagaccca cataactatg aaccatttga cccaagtgaa
     1081 gtaaatcttg aaagaaaaat agtaataggt aaacattcag gaagagctgc agttgtaaat
     1141 aagttgagtg aatatgaaat gtatatttct ccagaaaacg ctaccaagct attaaatgct
     1201 ataagagcta catctataag attaaaaaga agtttaatgg ataaagaaat tcttcaatta
     1261 tattgtgata tactagcaca tgagaaaggc acaacagaag aagaagcagt tagaggttca
     1321 tatatttaag atataattgt ttaggaggaa aatatgggag ataatatagt ttataaaata
     1381 attaaaaaac atatagtaga tggagaagct gttgctggaa gttcaattgg tataaaaata
     1441 gaccaaactt taacacaaga ttctactgga acaatgacat atcttcaact agaagctatg
     1501 ggaatagata aagtaaagac taaaagaagt gtggcatttg tagaccataa tatgttacaa
     1561 caaggatttg aaaatgcaga cgaccataag tatatacaaa cagtggcaga taaatatgga
     1621 gtttatttct ctaagccagg aaatggtatt tgtcatcaag tatttttaga aagattttca
     1681 acaccaggag atacactttt aggttctgat agccacacac ctactgctgg aggtgtgggc
     1741 atgatggcta ttggagctgg tgggttagat gttgctttag ccatggcagg aggagcatac
     1801 tatataaaag caccaaaagt ctgtaaggtg aatctagttg gaaaattaaa taatatggta
     1861 tcatcaaaag atataatatt agaagtgtta agaaaacaaa ctgtaaaagg tggagtaggt
     1921 aaagtttatg aatatggtgg agaaggtgtc aaaagcctat ctgttccaca aagagctaca
     1981 ataacaaata tgggggctga acttggagca actacttcaa tcttcccaag tgatgaaaaa
     2041 acactagaat tttttaagag tcaaggaaga gaagacgcat gggtagagct aaaaccagat
     2101 gcagatgcag tttatgatga agagattact ataaacttgg atgaattaaa accattagct
     2161 gcaaaacctc atagcccaga caatgtagat gaagttgaaa atataggcaa gattaaaata
     2221 gaccaagtgg caataggaag ttgtacaaat tcttcttatg aagacttaat gaaagtagca
     2281 caaatcctga aaggaaataa agtacataag gatgtgagtt tggtaatagc accaggttca
     2341 agacaagtta tggagatgat tgctagaaat ggggctttgg cagatataat aagtgctgga
     2401 gctagaatac tagaaaattc ttgtggtcca tgtattggta tgggtcaatc tccaggtact
     2461 gactctgtgt ctttaagaac tttcaataga aatttctatg gtagaagtgg gactttgtca
     2521 gcacaagtat atttagtaag tccagaagtg gcagcagtaa gtgcaatcaa gggagtgctt
     2581 acagacccaa gagaatttga tattaaattt acaaatttag atgtaaatga gtttttgata
     2641 gatgatagca tgataattaa acctgctgat gtaggaagtg atgtagaagt agttagagga
     2701 cctaatataa aacctttccc tcttaataca gagctaagtc agtctatagg tggaaaagtt
     2761 atattaaaga cagaagataa tataactact gaccatataa tgccatcaaa tgctaagtta
     2821 cttcctttta gaagtaatat accgtatctt gcaaactatt gtttcaatac tgtagataca
     2881 gaatttcctc agagagcaaa agacaataat ggtggattta tagttggtgg agataattat
     2941 ggtcaaggtt caagtagaga acatgcagct ttagcaccat tatatctagg tgttaaaggg
     3001 gtaatagtta agagctttgc aagaatccac aaggctaact tgataaatag tggtataatt
     3061 ccaatggagt tttgtgatga gaaagattat gaaaatatat ctcttttaga taatctggag
     3121 ataccaaata ttttagacaa tctaggtagt ggaattttag aagttaagaa tacaactaaa
     3181 ggaacttctt ttaaggtcaa agtagaattg tcagcaaaag aagttgatgt attaaaagct
     3241 ggtggaaaat taaactacac taaaaatcaa gctaattaat atgaaaagag ggaatattga
     3301 atgtataaag tgacacttat accaggagat ggaataggac cagaagttgc gaaagctatg
     3361 aaaaaggtag tagaagctac tggagtagaa atagaatggg aagaagttaa tgcaggagag
     3421 gcagttattg aagagtatgg tactccactt cctgaatata ttatagatag tataaaaaag
     3481 aacaaaatag ctataaaagg cccaattact actcctgtag gtaaaggatt taggagtgta
     3541 aatgtaactt taagacaggc attagatttg tatgtcaatt taagacctat taaaagtttt
     3601 aaggggataa aatcaagata tgaagatgtt gatttagtgg ttgtaagaga aaatacagaa
     3661 gatttatatg ctggaataga acataaaata ggtgattatg cagctgaaag tataaagata
     3721 ataacaagaa gtgcaagtga aagaatcgtt gattttgcat gtaactatgt taaagataat
     3781 aagagaaaaa aagttacagc tatacataag gcaaatataa tgaaaatgtc tgatggattg
     3841 tttttagatg tgtttagaga agttgcatca aaacatggtg tagaatatga tgatttgatt
     3901 gtagatgcag cagctatgaa cttagtttta aatccagaaa attatgatgt aatggtaatg
     3961 ccaaatcttt atggagatat actatcagat ttaggtgctg ggcttgttgg aggtttaggt
     4021 ataattccaa gtgcaaatat tggtaaagat tgtgcgatat ttgaagctgt tcatggaagt
     4081 gctccacaaa tagcaggtca aaataaggct aatccaacag cacttattca atcatcagta
     4141 atgatgctta gatacttagg tgaatatgaa aatgcacaaa aaatagaaac agctttagaa
     4201 aaagtatttc ttgaaggaag taaattgact gtagatttgg gtggctcagc ttctactaca
     4261 gagtttgcgg atgaggtgtg taagtatatt gtatagattt tatttaggga aaaagtaaaa
     4321 tatagattga aggaaagagg ctttctcaaa atagagataa gcctttttta ttggaatgat
     4381 aaaatatatt tgattttata caaaagagag ttcctcataa acgaaaaagt taattttggg
     4441 atatccattt taattttaaa attaattgat taatatgcaa agaaagatta aataaattta
     4501 ttatttttaa ttgaactttc aaccaaaata tggtaaaatt taaaaaagtt gaactttcaa
     4561 ctaaaaatga atatattgat tatgtgaaat tctataaaac agaaactatt tgactttagc
     4621 catagtagtt tcagtggagg tgtgtttgat taagacttta gacagtaata tattaagaga
     4681 agtaggtact ttatctagag cagtaaattc aataaatgat attaagtaca aggaattaaa
     4741 gttacaaaaa ggacaattta catttttgac aagaatctgt gaaaatcctg gtataaatct
     4801 tgtggaatta tcaaatatgt taaaggtaga caaggctaca acaaccaagg ctatacaaaa
     4861 attaataaag gcaggatatg tagataaaaa gcaggatgag tttgataaaa gaggatataa
     4921 tttaactcca actgataaat ctttggaagt ttatgagttg ataattgaag aagagaatag
     4981 aagtatagag atatgttttg ataattttac agatgaagaa aaacaagtgg taactaagtt
     5041 attagaaaaa atgagcaaaa acgtagaaaa tgaatggttt aaagtaaaga gatagtaaat
     5101 aaatacatta acttaaggag atatttttat gattagaaaa gcgaatatga atgatttaga
     5161 aagcattatg aaaattataa aatctacagt ggaagaaatg aagacttata ataatacaca
     5221 atgggatgaa aactatccat tagaaaaaga ctttgtttca gatattaaga aacaagattt
     5281 atatatttat gaagtggatg gagaagtagc agggtttata tgtcttaatt atgaagagcc
     5341 agaagaatat ttaggcttaa attggtcatc taataaaaaa gcaatggtaa tacatagaat
     5401 ggctgtaaat caaaacttta gaaagacagg aatagcatct aagttagttg attttgctga
     5461 aaaattagct gtagaaaaca atgtatcata cttaaagagc gatacatatt caataaactc
     5521 aaaaatgaat ttattactta caaagtgtgg ttttataaaa atcggagaaa tgagtttttt
     5581 aggaaaggag aaatctttct actgttatga taaaatatta taaagagcat ttacaataca
     5641 aggggtaaat aatagatttt cagcattagt aagggcataa tataacctag tatatgctat
     5701 tatgaaaggg atgatttata tgattggatt tctaaagaaa agaagaagta ttagaaaata
     5761 caaggatgtt gaagtagaaa aagaaaaatt agataaaata ttaaaagctg ctcttttagc
     5821 tccatcatca aaggggctta gaacatggga atttatagtg gtagatgata aagaaaagct
     5881 aattaattta tctcaatgta gaactaaagg tggtggattt ttcttaaaaa atgcaccttt
     5941 agctattgta attattgcag ataaagaaaa aaatgatgtt tggatagagg atgcaagtat
     6001 agctgcttca tacatacaac ttcaagctca tgaactaggt ttaggttctt gttggataca
     6061 agtaagaaat agaatgcatg atgataatat agaagctgat aagtacatta gagaagaatt
     6121 aaaagtacct agcaagtaca gtgtagaatg tataatttct ataggatatc ctgatgaaga
     6181 aaagaaagca tacggtgact ctgatttaga ctataaaaag gttcatttta acaactttta
     6241 gtatataata tgttgtatta agacattata tttttttcta ttgttaatat atacttaaaa
     6301 ttgtaaaaat atatctgaat tttactaact tatctagggg ttatgtacaa ggattttaaa
     6361 gataaatata gaaattaatg tttataatcg atacataact tgagtaacaa aatttaagga
     6421 ggttaaagta tgggtatatt taaaacaaaa atggatgagg actggaaagt aaactacatt
     6481 aaagagttta atgaaatgag agattcttat gagagtaaac ttcaaaaaaa acagtttgag
     6541 gtagatagtt tgaaatcaga attggataga ctaagaagct ataaaaatag ccttaagcca
     6601 aaagaaaaac aaattactga tgatgatata aataatataa agagtttaag aagagatggc
     6661 ttaagctata aagaaatttc aaatcaaaca agttggagca aagcaactgt aagtagggta
     6721 ttaaatggac tttacgatta gcatatacat ggggtgattt aaattgggat gaagtatagt
     6781 tacaaaaaat gcataataga ttctatttta ttgatgttta tagttcaaat attaaggatg
     6841 attttgaatt atgtacttct aagtcaattt gaatttactc ttgaaaattt taatattata
     6901 aacttaattt cttttacatt agttggttta tcgttaattt tatttttaaa agataattct
     6961 ttgtataaca aggttagaaa tagaaaaatc acagaagctt ttgaagaaaa taaaaacaat
     7021 atacttattg aaaagtgtaa gctgatactt tttgttgtgg ttttatcttt agcaataatt
     7081 gtaacttatt gtactaaggg atatgtactt tttaatgtaa ctatgatgac tttgtctgta
     7141 ttgatagttc caatatttga agagttattt tttagagaat atatatggaa ctatctaagt
     7201 aattttataa agtcaaaggg taaaattatc tgtataacat ctatactatc tggaatttat
     7261 aatataggat acatagatgt tataagaaat tatgtaatat tgtataacaa ttcaagctac
     7321 acctttgaag ttattatttc aaaaattatg ataggtactg tttttggtat tgtacttgga
     7381 ttagttaagt ataggtttag agatgtaggg ttttgtatat tattgaggag cttatttgct
     7441 atatttatta ggtagatagt ataatcggtt tataaggttg ttttaaattt aattttaaat
     7501 caaccttatt ttatttgtgt tttataaagc acttatatat taataactaa taaatttaat
     7561 aaaaattatt tgaatatttt taaaatatat aaatatgtat tgacatataa atattatgaa
     7621 ttacaattta tataaactaa taaaaattaa ataatattaa tcaacttatt gaatggagga
     7681 ataaaaatga aatttgtgac tttttgtagt agcaatgaag aaaaaatagg tgtatttaat
     7741 tcagaaacaa atagtatata tgaaattaat agtttaggat taagtaaatt atatactgat
     7801 atgaatgatt ttattgaaaa cgtatctaca ggagatttag aaaaaataaa aaacaattca
     7861 tttgaaaacg caaagtgtta taagttagaa gaagttaaat tatgttcccc aattgtaaga
     7921 cctaaaaaag atattatatg tttaggtcta aactataaag accatgtcaa tgaaatccca
     7981 gatggagtta taaaaaacgt agttatgcca gattatccaa tttatttttc taagagagca
     8041 gacaaaataa ttggagtgga tgataagata agcttacatg gagatttggt agagaagttg
     8101 gactatgaat cagaacttgc agtaatcata ggtaaagaag gtataaatat atcaaaagaa
     8161 gatgcttatg aatatatatt tggatataca atagtaaatg atataagtga aagagcactt
     8221 caagataaac atgttcaatg gtttagagga aaaagcttag acacacatac atctatggga
     8281 ccttgtatag tacataaaga agaatttgaa catcctttaa aattagatat aagtagtgta
     8341 gtaaatggag aagtaagaca agattctaat acagagtact ttatatttga tatacctact
     8401 ataataaatg atttatcaag aggaatgact ctaaagccag gagatataat ttcaactgga
     8461 actccagcag gggttgctat ggggatgaat cctcaagtat atttaaagca tggcgatata
     8521 gttgaatgta aagtcgaagg aataggagtt ttaaaaaata tagttgatta atggataaat
     8581 atgccatata attttattag tacgaataat ttaattaata taggattaat caaacttatg
     8641 attaatttat tatagagagg gatgagtata tggcaagaga actagaatta aagtatggct
     8701 gtaatccaaa tcaaaaacca tcaaagattt atatgaagaa tggagaactt cctatagagg
     8761 tattaaatgg aaaacctgga tacataaatt ttttagatgc atttaatagt tggcaacttg
     8821 tgaaggaatt aaaagaagct acaggtcttc cagcagcaac ttcatttaaa catgtaagtc
     8881 cagcaggagc agcagtaggg gtacctttat cagatacttt aaagcaaata tattttgttg
     8941 atgatttaga gctttctcca ttagcttgtg cttatgcaat ggcaagagga gcagatagaa
     9001 tgtcttctta tggtgatttt atagcacttt cagatgtatg tgataaggag actgcaacta
     9061 ttatagcaag agaggtttct gatggtataa tagctccagg atatacagat gaagcccttg
     9121 aaatattaaa aggaaagcgt aaaggcaact ataatattgt aaaaatagat gaaaattata
     9181 ctcctgaacc tattgaaaca aaggatgttt atggaattac ttttgaacaa ggtagaaatg
     9241 aaatattaat aaatgaagac ctattaaaag atattccaac tgataataaa atttttacag
     9301 atagtgcaaa acgtgattta attatagctc ttattacatt aaaatataca caatcaaatt
     9361 ctgtttgtta tgctaaagat ggacaagtaa taggggtagg tgcaggtcaa caatcacgta
     9421 ttcattgtac tagacttgca ggtaacaaag ctgatacttg gtatcttcgt caacatccta
     9481 aagtattaaa cttaaaattt aaaaaggata taggtcgtcc agatagagat aacactattg
     9541 atgtatatct ttctgatgat tatatggatg tgcttgctga tggcatatgg caaaactttt
     9601 ttgaagaaaa accagagcct ctaactggag aaggaaaaag agcatggctt aaaactttaa
     9661 caggagtagc acttggttca gatgcatttt tcccatttgg agataatata gaacgtgcaa
     9721 aaagaagtgg ggtaagtttt atagcacaac caggtggttc tattcgtgat gataatgtta
     9781 tattaacttg taataagtat aacatagtca tggcatttac taagaataga ttattccatc
     9841 attaaaatac tgaaagtata aagataatat agattaagta aaaatctaca acaagttttg
     9901 ttgtagattt ttttatttgc tgtataattt taaatagaac tttattttta gatattcaat
     9961 ttggaggttg atatggcttt aaatataaga aactatagcc tattatgtaa tcaaggaaca
    10021 gctaaattta atgaagatgt agtcggtata aatccatttg gcgcatgggt tttagatggt
    10081 gctacaggac ttaatggaaa aaatttagtg tcaactgaaa gtgacgcaag gtggtatgtt
    10141 cagtggtgga ataggtattt atataaaaat ataaataaag aagaatcatt aaagagtatt
    10201 attaatactg gaataataga agtaaaaaaa gagtatgaac taagattaaa tgggtttaag
    10261 gttgaaaaat tagatttacc atctagttct atagttgttg taaaatttca tgaaaataaa
    10321 gtagagtatt ttttattagg agattgtgct ttgtttatta aaaatggaga ttccaaagtt
    10381 ataaaagata ggagtatttg taaatttgat aatactgtat tttatgaaat gagtaaactt
    10441 tctaacttgg aagagatggc atttgatgag attagaacta gtgttatgga tacaatagta
    10501 agcaatagat tgaagaaaaa tacaaaagag ggctattgga tattggattt tgatgaggaa
    10561 gctgtggaaa attctatata tggatacatt gaaataaaaa aagattttca attgatgcta
    10621 acgagtgatg gttttacaag tgcatgtgat agatataatc taataaaaga agaagaattg
    10681 atacgtatag cagaaaaatt tggtatagga tatatacaca ataaagtaag ggattttgaa
    10741 gataatgact ataaagcggt taaagtgcca agatttaaag taaaagatga ttcatcttgt
    10801 ttatatttag atatatataa tgattagact gtataaaact atttaatttt agaaaggagg
    10861 caagtgaaaa gtgaaaattc ttcatataat aacacaaaaa ccaaatagta caggtagtgg
    10921 tatatatttg tctggtatga taaaagggtt cgaaaaaatc ggtcataagc aagctgtaat
    10981 tgcaggtata gatgtaaatg atgatgtaaa ttgttttcca agtgaagtaa gtttttatcc
    11041 agtaaagtat aattgtgggg agttaaattt tcctgttgta ggtatgagtg attctatgcc
    11101 atatgaaagt acaagatata aagatttaaa tatagacatg ataaatagat tgaaatatca
    11161 atttaaagta aatatagata aggctatgaa tgattttaaa ccagaactta taatatgtca
    11221 tcatttgtac cttttaactg catttgttag ggaaatggta aaagatataa aggttatgtc
    11281 tatttgtcat ggaacttgtt taagacagtt aaataccatt gatttagaga aagaatacat
    11341 aatagcaaat ataaggaaat tagatttaat atttgctctt catgaaaatc agaaatatga
    11401 tattataaag acttttggag taagtgaaag taaagtagtt gtaattggta gtggttataa
    11461 tgatgatata ttttataata aaaattataa aataaaggat gataaaataa aaatagtatt
    11521 tgcaggtaag atatgtaaat caaagggact gattccattt ataaaagcta tatctaaatt
    11581 aaaatatagt aaagatttaa tagaagtcaa ttttgcaggt actggaagcg atatcgaatc
    11641 atataatgag atagttaaac tggcaagtaa gtctccattt aaaatgaatt tcctaggaaa
    11701 gttagaacag agagatttag ctgagctatt caacaggtca caaatatttg tgttaccatc
    11761 attttatgag ggattaccag ttgtagtttt agaagcttta tcttgtggca cagatgtaat
    11821 cactactgat attcttggtg ttaaagagtg gattggtagt gagattaata attctggaaa
    11881 gatagaatat gtttctctac cttttatgga gaaagaaggt attccaaaag atgaagagtt
    11941 atatgatttt gaaaataatt tgtataatgc aatagattct aagatacaat ctttattgaa
    12001 taactcaaat aagaaaacat ctgtagatat gtcaaaaaaa acgtgggatg gcttagcata
    12061 tagaataaat gaagttattt taatggatga attgtgttta gtctaaaatt atttaaatta
    12121 taaaaaagtg taactatttt tacactattt ttaaatgatt ttatattata ctaatactaa
    12181 atatatttgt tttagggagg taatttatga ggattagtaa aaaggtattg gcgttaggta
    12241 tttcagcatt actgttaagt gttagttttc caacatcaat aaacgcacta gataagattg
    12301 aaaatattca aggagtagat aaatatgaaa cagctgggct tatttcagat aagcaagatt
    12361 ttacaacagc agttcttatc aatgcagatt ctacaatggt agatgggatt gctgcaagtg
    12421 gtcttgcagg agtaaataat gcagctattt tattaactaa taaggatgat ataccagaag
    12481 caacgcttca aagactgaat agagttacta agatatatgt aataggtgga gaaaattcta
    12541 taagtaaaga tgtagaaaaa atgttactta tgagaagaat gcaagttata agaattgatg
    12601 gtgtagatag agtagataca agttataaaa ttgcaggtga aatagaaaaa attaaaaaca
    12661 gtgataagat gtttttagta aatggattta aggatgaagc tgatgcagta agtgttgcat
    12721 ctgttgctta tagagatgga gctcctataa tattgacaaa agatatacct tcagcagaag
    12781 aagacacaga cttagaccca tggtttggag tttctcctgt tccagtatat gctattggag
    12841 gagagtctac attaagtgat tacatagtga gcagatatag agcaactaga attggtggtg
    12901 ttgatagata tcaaactaat aaaaatgtta ttgaaaagtt ctataatgga gcaaaagagt
    12961 tctatataac aagtggagac gatttagttt atgctttagt tgcatcacca cttgcaaaaa
    13021 atgctcctgt tgttttagtt tcaaataaaa gtgataagag tatattaagt ggagctagta
    13081 aagtaacagc aataggtata agtgataaga gtattataga gcaatgtttg gatgctgtaa
    13141 agaagtaatg gagtgttatt agtaaaatga atatacacat tgaaaattag tagattgttc
    13201 attttataat ggtataaaat atatgggaag aggctatcaa atggagataa gcctcttttt
    13261 cattgttaaa attatgaaaa gatactttat tttatataaa aaagtatctt atgaactaaa
    13321 tgtctatttt gatatactat ctccttttct tatattatat tattttttat atggaataaa
    13381 tttcataagt attatcttcc tttaattttt ctaaccaaac catctctaaa atatctagtt
    13441 cttctaaata gtctttctct gttttgttat catcatgttt tataagttct aaaacttcaa
    13501 atgtaaatgc tttttcacca tattttttcc attcattatt taattctttt atcatacatg
    13561 agccagcacc aagctgaaat ttgagagagt taaatttgct ttttatgtca ttactttttc
    13621 caaggtaaat ctttttaata atatcatttt ttattatgaa tacacctgtg tcaaatttca
    13681 tttctttgta taattgtttt aattcacgtt ttctatccat gattttagat acctctcttt
    13741 ataattgata tgtaatatgt aagtgtaaaa atatattaca tatcaattat aaagagatta
    13801 attttatatg taaatagttt ttataaattt aattatgaat tagtcatcaa taaacaagaa
    13861 atttagctgt agaaatccat tataaaggct attataaatt ttaatatgct aaaaatataa
    13921 aaagaatagt tgacagtgta gaaaatatgg aataaactaa aagtgtaata gtaatatgaa
    13981 attatatatt acattgaagg gtgtgaatga ttattatgaa taaaaaaata gaattaatag
    14041 ggaactttgc aagtgattgg attaggtata gtgattatga atataaaatt actgaagaag
    14101 atgaattata tatagttcca acagaagatg ctgtgtttac catgtataac ccatttgatg
    14161 tagcagatga tataatagta gatataattc gcataggaca agaggcatta caagatatat
    14221 caaaagagac aaaagaaatg aaactaaaga aagaagtact tgaatttgta aaaaagtatg
    14281 gattattggg tttaatatat tcaagtgtat ataatagaga tatagttgga gaagaaagag
    14341 tactgatgat tgaaaagaat tatattacaa aagagaatat attgagtaca gatgaatatg
    14401 taaacatgtt tataccattt gcaagtgatg aagatgtgta ttttaaaaag tatagaaggg
    14461 gtatagatgt atcaaagaga gaagaatctc caaagttttt tggaaagaga ccattgattt
    14521 tagatttagt attttcaaag ttctacactg aacaactaga ttggattgta agttttgcta
    14581 aagatatgtg taagcatttt aatcaaattt gtgtatataa aaatacatct aatctattaa
    14641 ctgaaagtgt tactattatg tctggagggt ttaatcctca aaagatagga ttttctataa
    14701 gccaattaga taaaccaatt attgcatggg agtttgattc tttgaaaaca gctattgaga
    14761 ctatctatgc ctttgcaatt acaagtgaac cttcttcttt aaataaatgc aagtactgtg
    14821 gtaaaattta tattcctaca aattctaaat ctcaatactg ttcaccttct tgtagaaatt
    14881 gctcaaatgt aaagaaaagt agaagtagag ccaaaaaaga taaataaaat taataaatat
    14941 atcattgttt agattttagt aaataactac tgaattaaaa gttgttatag aaaattatat
    15001 atctaaatga tggtattttt tttgttgaga aaattgaaaa gtttaattga gatagtaatt
    15061 ttgagttgac aaaaaccact tttaacacta ctattataag tggttggcaa aataatatat
    15121 actaatttaa gtatagcata aaaaacaaaa aaagtcaatg gaaaaaataa aaaaggagaa
    15181 tttaaaaatg atatcaccta ttattttgag ttctataaat cagaatttaa aggaaataga
    15241 aagaaatgaa ctgcttgaaa ctaatataga atctggagat tatggtctag ctctttctga
    15301 atctgatgta aaagatataa taaattcgag agataatact ttaaaggggt atggaagaat
    15361 agaattagat ataaaagtta caaaacagtt aatagaaaat atatatacat cacaatatac
    15421 aaatgtggac aattatttag aggctattaa tgatatgcaa gaaatttttt attatttaaa
    15481 aaatgaaact gatgacaaga tatgtgatga tgagattata gaaatactgg gtgaatttta
    15541 tgaaaaattt tcaggcaata tggacaatgt aagaggagaa gcagacgaat ttgcaaagaa
    15601 atttaaattt ggagaagttt aatatatgat aggagagatt ttgtatgtta aataatttaa
    15661 gtaatattaa tataaacgaa aataatttaa taaagaacca atattccata tctctactta
    15721 aggaatgtct aaattgtaaa gtgatagatg aaagagaagt atataatata caacaagaaa
    15781 tatctctaat acttatggat ttgattaaaa aatatacaaa tggtcaaagc acatcagtaa
    15841 aaacagaagt tgccgaaaaa ctgttgattt caatatggta tgctattgat gcatatataa
    15901 ataagcttgg agatatagaa aaaagagttg tggagttgac tgctaagaat gttaaaaagg
    15961 tgtaccttgg aggcattgaa atattagagg aagagttagt taggctaaaa gatttttata
    16021 aactaatgat gagtagaaaa ttggatacta attcaatagc atataatgat accttgatgg
    16081 agttatcaag tttctttaaa agttatattg taaaatttga agctcaggac gttccagtaa
    16141 gtatagatta tccattggca ctagatgata tggatgttaa gggtatttat tatgtgaaga
    16201 attatattga gagtatagat atagaaaata gattttgtaa cctatttaag aaaaaagata
    16261 gagaaaaatt attttatgac tatggggtga cttataaaat agattgtgat gttatacttg
    16321 taaatttatt tgaacttatt ataaataatt gtattttttc tactatttta ggaaatgaag
    16381 tgactaattt agaaatttca aaatatgagt atgaattttt agaaaaacaa tttaaaaata
    16441 tgtacaataa tattactgaa gataaagtta tagactctga agaaagtaat ataattgaat
    16501 tgattgaagt agatgatagt tttaacactg aagtagatag taatttggat tataatgatt
    16561 ttgaagaaat taacagagaa attagaataa ataaaaaaat aactttacta cttatggaag
    16621 caattgaagt attaataaaa agactaagca tagaagatga agatgtaata agatatatta
    16681 aaaaatatga aaagatattt attgaatctg tgctaagctc aataaaaagt aacactttta
    16741 aaagtatggt aactgtgagt aaaaataaaa aggaacactt agaagattat attgttgatg
    16801 atgaaagtaa attggatgat gaatcgttta gagagatatt caatgagata ttagatagta
    16861 catctataat tgaaaaaata agaataataa aggaaaatat caatgctaaa aaagacttta
    16921 tcgatattct tgaatctgaa tgtttatttg gagaagagta tttaatgttg tttgcatcat
    16981 taagtgaatt agaattggca atattgggta gtgtagtatt ttatgaagat attagaatga
    17041 gagaaattaa tatattagaa tttatgttaa atgagaaaac tgaaactact ttatggaaga
    17101 tagagtacat taaatttatg aagaggcaaa gtgaagataa gatatactcc atagaagagc
    17161 atatgaataa aattaattaa caaaaaattg tgaataaata gaatgttagt atataatata
    17221 atctgtaaag catatcaaaa atacatatat ttggaggaaa attatgaaag agtatatcat
    17281 aaataatgtt gaagaaataa gagaagaatt aattgattta tctaagaaaa tatgggaaaa
    17341 tccagaatta gcctttgaag aaaagtatgc aagtagcatt caaaaagaat acctaaagtc
    17401 aaaaggattt aaaattgaag aagtagaaaa tttacctact ggatttatag caagttttgg
    17461 agagggtaaa cctgtgattg gcatacttgg agaatatgat gctttaccag agctatcaca
    17521 atgtgtgagt gcagaaagaa agccattggt agaaggaaaa gctggacatg gatgtgggca
    17581 taacctgttg ggtgtagcag gtgtaggagc agttgtttct ataaaaaaat taatagaaga
    17641 aaataaattt aatggaacaa taaaatattt tggttgtcct gctgaagaag aaggtggagg
    17701 aaagacagtt atgtgtataa atggctgttt tgatgatgta gattgtgcat ttacatggca
    17761 tccttttgat ataaatgcac cttggagagg tggaagtctg gcaaacttat ctgtaaaatt
    17821 taaatttaaa gggataacag cccatgcagc tcaagctcct cataatggta gaagcgcact
    17881 tgatgctgtt gaaattatga atgttggagc taattatttg agagaacatg ttattgactc
    17941 tataagaatg cactatgtta taactaatgg tggtggaaga cctaatgtag ttcctggatt
    18001 tgctgaaagt tggtacttta ttagaggtaa aaaagcaaag gatgcagaac acgttcttga
    18061 tagattaata aaagtagctc aaggagcagc aatgatgaca gaaactgaaa tggagtataa
    18121 ggttacagat ggaatatatg actatatacc aaaccaatgt ttgactgatt tagtttatga
    18181 caatatggtt tttgttggtt gccctaaaaa tactcctgaa gaagaagaat ttgcaaaaaa
    18241 attgtgtgat actcttacta gagaagaaag attaggtgta gcaacatcat tacaaaatga
    18301 taaatctatt gtagaaagct atatacacaa ggaaatagtt gacggtgaca aagataaagg
    18361 actagctggt tctactgatg ttggagatgt aagttatgta ataccagtgg ctcaatttgc
    18421 aatggcagct tggcctgttg gaattgcaag tcatacatgg cagtcatgtt catcagcagg
    18481 ttcaaatata ggattttctg ctatgataaa ttcagctaaa gtactggctt gtagtgctta
    18541 tgatgtattt atggatacta aaattattga tgaagcaaaa attgaatttg ataaatcatt
    18601 agatggacaa aaatttaaac cattggtata attttggtaa taaaatattt ctggggggaa
    18661 tagctatgag agaaaaagtt gaaaaagtgc ttgaggaaaa gataaaacca gtattacaaa
    18721 gagatggtgg agatgtagag cttatagatg taaatgaaaa tggtgtggtt ttagttagac
    18781 tacaaggggc atgtagtggg tgtccaggag cgactatgac aatcaaagct attattgaaa
    18841 atgttttggt gagtgaagtt ccaggagtaa ctcaagtatt aggtgtttaa tagttaacgt
    18901 ttattaaatg tatgttatac ataagtattt aataaagaag taaaaaaaat agtgaagcaa
    18961 tatatatatg aaaaagtatg agaaagataa taaaacattt atagaaataa tgaaatattt
    19021 catatataca tatgaagaaa tagtaaaaca atatatatat gaaaaagtgt gagaaaataa
    19081 taaaatacta tagaaacaat taaatattct atataggtat taaggaacat gaaagaaata
    19141 atggagtatt ataaataaat atgtaaaagt gtgaaagaag gatgtagata aatttgtctt
    19201 atacaaattt acctacagta tttcttacac actttttatt ttactatcca gccataccaa
    19261 taaacatacc tataaaatat ggaactaacc atatagtcca tacaactata ctaaacttat
    19321 gaaagaattt tttcttttcc tcatcatctt tgtataggac aaaagtagcc caacttgcat
    19381 gaaaaagcat aagcacaatt gcaagaagac ctgtaatact atgaaggttt tgtgataaag
    19441 cagattttac ttcaaaagta tggctgttgg ctatgttact cattgtaaat gttcctaaag
    19501 tatcaaatat aaaaccaagc caaaatataa ttacatgttt cttttttaaa atttttgcct
    19561 ttctttctcc aaatacacct atagtgtaga aaattaaagc tgatgttata aatactattg
    19621 caagtattaa tttataattc ataattatct ctcctttact tttgccaata tatttctaag
    19681 attttttact aagtcactca attcgttttc atcacaattt ttaaaaatat catcaaaact
    19741 ttcttgcatt ttaattttaa aatttgtggc aaattcaaat cctgttgttg taaacttaac
    19801 gtatgtattt cttttatcat tagatttctt gaatttttct atatatccaa tctgttctaa
    19861 tctgctgatt atgcctgaaa ccgtaccttt agctaaagac atttcatcac atagttgtga
    19921 tattgtaagt tcttgattat gagctatcag ttttataact ataatctgtt ggtgtgttaa
    19981 tccattttct ttaaaatttt cttctactgt gtacatagtt ttagaataaa gttcttttaa
    20041 gagcatggct attctaaaac agtcataatt acttttcaat aaaatcacct cttaaatggt
    20101 ttgcatacaa actaatataa tatacttttc ttatatagtc aacctaaaat tttataattt
    20161 tatagaaaat aatgaagaat agaatataaa aattttattt taggtataaa taaaataatt
    20221 tgatgaaatt ttaacaattt ttaaaaagtt tgtttacaca gttaataaat gatgctaaaa
    20281 ttaactcata gataaatata gaaaatttaa ttttgtttaa tgataatgga accacgaagg
    20341 ttttaacgtt actttacgtt tgcttttatg tggttttttt tattgcaaca aaggggggtt
    20401 ggggtttgtt aaaggatata ttgaaaaagt tgttgcagtt tattttagta atgtttttat
    20461 tatcttttat tgttttttac atggcaagac ttgctccagg agacccatta atctcttact
    20521 atggggatgg agtagaaaga atgagtacac aagaaaaaga aaatgctatg aaaaaacttg
    20581 gtttggatga accaatatac agtcaatata taaaatggat ttcaaatgca tcaaaagggg
    20641 aatttggaat ttcatttaaa tataaacaaa atgttacttc tgttataaat gatgtctata
    20701 taaacactat aatattaggt ggttcaggat atatacttac ctttgtgcta gccttattgt
    20761 taggaatatt ttgtacatta catgaagata agctcattga tagaatcata tgcaaattag
    20821 gaacaataac caactgtatt ccatcatttt gggttgcctt agtactcata cttatattta
    20881 gtataaactt aagtatactt ccaagtagtg gtgcttattc aatgggagaa gagtccagta
    20941 tattaagtag gatatcacat ctaatcttac ctcttacagt acttatatta agccatctat
    21001 ggtattacac atatatgata agaaataaat tactagagga aataagagaa gattatgtgc
    21061 ttttatgtaa agcaaaaggt ttaaataata gaactattgt ttttaagcat tgcctgagaa
    21121 atataatgcc atcatatatt agtataatgg ccatttcgat acctcatata ctaggaggaa
    21181 cttatgtagt tgaaaaagtt ttttcttatc ctgggttagg aacactttgt tttgaaagtg
    21241 caaagtatca tgattataat atgttattag ttttatgctt aataacaggg gctttagtag
    21301 tatttgggaa tatgttagct caaattataa acaataaaat agaccctaga atgaaatatg
    21361 atagaggtga tactaatgaa gccacaatat agtgattttg aaattgtagg agaaaattat
    21421 attttagtag cagaagatga acatatagaa acaaagaaaa ccttatctaa aaaaattaaa
    21481 gaattaccat atatatcaat tattatttta tcaatcatag tcattggaag tgtattctca
    21541 tcttttataa tgacacatga accaacatac atggatttag caagttctaa tttagctcca
    21601 aataaaactt ttttatttgg aacagactca atgggtagag atatatattc tatgatatgg
    21661 tatggaggaa aaatatcttt atttatagga ctattttcca ctgtaatatc aactgttata
    21721 ggaattgttt atggtagtat aagtggttca gcatctgaac tcgtagatga tgcaatgatg
    21781 aggtttacag aaatcatact tagtatacct tcaatattaa ttataatatt tgtacaagct
    21841 atacttggaa attcaaatcc agtatctatg tccatagtaa tagggataac aagttggatg
    21901 aatatatcta agatagtgag aacagaagtt agacaaataa gaaatagcga atacatacta
    21961 gcagctaaga gcatgggagg aggattcttc tatgtattaa agcaacattt acttccaaat
    22021 tttgtggcat caataatgtt catggttgta acaaacatag gcgcagctat tggtactgaa
    22081 tcgacactta gctttttagg aattggttta ccaatagaaa ttgtttcttg gggaagtatg
    22141 ttatctttgt cagaggaagc attactttca aatagatggt ggattatact gattccgggt
    22201 atatttttgg ttacaacatt ggtgtgtata acaaatatag gtaattatat aagaaagaat
    22261 aataataaaa aatctagtaa tttataaatc agcatttgag tactaaataa gcaaatactg
    22321 aggtatttga aatatctatt taaataaatg gggtcaaagc tttgaaaaat attttataaa
    22381 aagcaaaagg gggagcatca tgaaattaaa gaagttaaaa gttttaagtt tagtaatgat
    22441 acttagtcta atggcaggtt gttctagtgg aggagacaaa gataagaagg cagatacgcc
    22501 taaagatgga aaggtacttg tttatggaag taatgactat acaagtataa atcctgcact
    22561 atatgaacat ggagaaataa attcgttaat atttaatgga ttaacagctc atgatgaaaa
    22621 taataaggtg gttccttgtc tagcaaagga ctggaaattt gatgaggcaa caaatacata
    22681 tacttttaat ttaagagatg atgttaagtg gcatgatgga gagaaattta cagcaaatga
    22741 tgttaagttt acaatagaaa ctataatgaa tccagataat gcttctgaga tagcatcaaa
    22801 ttatgaagat ataacaaaaa ttgatgtggt taatgataat actataaaaa ttactttaaa
    22861 agctccaaat acagcaatgc ttgattattt aacagtagga gtattaccaa aacatgcttt
    22921 ggaaggtaag gatatagcta ctgatgaatt taatcaaaaa ccaataggta ctggaccatt
    22981 taaacttgaa aaatgggaca aaggtcaaag tataacactt gttaagaata gtgattattt
    23041 tgtaaaagaa ccaggattag ataaagtagt ctttaagatt gttccagatg ataaggctaa
    23101 ggcaatgcaa ttaaaatcag gagaattaga cctagctcaa ataactccta aagatatgtc
    23161 taactttgaa aaagacgaaa agaattttaa agtaaatata atgaaaacag ccgactatag
    23221 aggtatatta tacaatttta attctaaatt ctttaaagat aaaaaagcta aaggattacc
    23281 aaatgcttta agctatgcaa tagatagaaa agcaatagtt gacagtgtat tattaggaca
    23341 tggtgtacca gcatattcac ctttacaaat gggaccatat aataatcctg atatagaaaa
    23401 gtttgaatat aatccagaaa aagctaaaca agaaattgaa aagctaggtt ggaaattagg
    23461 ttctgatgga atctatgaaa aagaaggtac taagttagct ttcgagataa cagcaggtga
    23521 gagtgaccaa gttagagttg atatggctaa aatatgtgca caacagttaa aagaaattgg
    23581 tgttgatgct aaagctgttg ttgtaactga aacagattgg gctaatcaag atgctcattt
    23641 aataggttgg ggaagtccat ttgacccaga tgaccataca tataaagtat ttggaacaga
    23701 taaaggtgca aattacagtg cttactctaa tcctactata gataaaatac ttcaaaaagc
    23761 aagagaaaca gaagataaag atgaaaaatt aaagttatat aaacaattcc aagtagaaat
    23821 gactaaggat atgccttata catttattgc ttatatagat gcaatatatg ttggaaaacc
    23881 aaacataaaa ggattaacac ctgatacagt tttaggtcat catggtgtag gtatattctg
    23941 gaacatagct gactggacaa ttgaataata ctgaaattaa ttagaaacct tagggataaa
    24001 aatatatccc taaggtttga atagcatagg ggttgatggt ttttggaaca tttattggag
    24061 gttaataatc tatctgtaag ttttaaagta gaagaaggag aagttcaggc tgtaagaaat
    24121 gtatctttta atctaaaaaa aggtgagaca ttggctatag ttggagaatc tggttgtggg
    24181 aaatctgtac tgtgtaagag tttgatgagg atactcccat acaatggata tattaaaaat
    24241 ggagaggttt tacttaaatc aagtgatttg gttaagaagt ctgaaaaaga gatggaagat
    24301 ataagaggaa agaatatatc aatgatattt caagacccta tgacatctct caatccaact
    24361 atatcaatag gaaagcaaat tgcagaagct gtaatcattc atcaaggtat aagtaaaagt
    24421 gaagcaaaaa agagagccat agagcttatt gaattggttg gaattgataa tcctgagaaa
    24481 agatttaaac aatttccaca tcatttttca ggtggaatga gacaacgtat agtaatagct
    24541 atagcacttg cctgtaatcc agatgtattg attgctgatg aacctacaac tgctttagat
    24601 gtaactatac aagctcaaat aattgattta ataaaggatt tacaacataa aataggtttg
    24661 tcaataatat ttataactca tgatttagga gtagttgcaa ctatagctga tagaatagca
    24721 gttatgtatg caggaaagat tgtagaaata ggaactgtag aggatatatt ttatgaccct
    24781 agacatccat atacttgggg gttgttgggt tcattaccta ctttagattc gcaggatgac
    24841 tatttatata atatacctgg aatgcctcca aatttattga acccaccaaa aggagatgct
    24901 tttgcaataa ggaataaaaa tgcacttaag atagattatg aaaaagagcc accaatgttt
    24961 aaaattaatg atacacatag tgcagcaact tggttattgc acccagatgc acctgaagta
    25021 gatgtaccag ttagagtaaa ttgtggaagg gtgatttcta atgaatagag aaaaaatact
    25081 ggaaataaaa aacttaaagc aatattttca tttggacaaa agtactactg taaaagcagt
    25141 tgatgatata agttttgata tatataaggg tgagatattt ggattggtag gagagtcggg
    25201 ttcaggaaaa tctacaactg gtaaaactat aataagacta catgaatcta ctggtggaga
    25261 agtaatttac aaaggaaatt gcatatcaga taaaaaaacg tacaaattta taaaaaaaga
    25321 tgtaaacaaa agtatgcaaa ttatttttca agattctact tcatcattaa atccacgtat
    25381 gacaattgca gatataattt cagagccact aaaaattcaa ggtatctgta aaaataaaac
    25441 agatagaatg aataaagtat atgagatgct aaagctagta ggacttgata gaagttatgc
    25501 aaataagtat ccatctgatt tttcaggtgg acaaagacag cgtattggca tagcgcgagc
    25561 tctttcagta gacccagaat ttataatagc tgatgagcct atagcttctt tggatgtgtc
    25621 tatacaagca caaatagtaa atttatttaa aaaattgcaa caagaaaaga atttaacttg
    25681 tttatttata gctcatgatt tatctatggt gagacatatt agtgaccgta taggagttat
    25741 gtataatgga aaattagtag aattggcaga ttcaaatgaa ttgtataata atcctattca
    25801 tccatataca aaatcactac tttctgcaat tcctgttcct gaccctagat atgctaagtc
    25861 aagaaataga atagagtata attctaatgg atatgattgt tcaaatgaaa agtcattaag
    25921 ttggattgag gtttcagatg gacattttgt atattcttct aaatcagaaa taaataaata
    25981 tcaacaaaat ttaaaagttg tttagtataa aaatacttga acaggtattt atttgaaata
    26041 caaactaaaa ccctattttt ggagaaattt taaaactact ccaaaatagg gttttttatt
    26101 tctacttttc aaatataata aattcaaata taataaaatt aaaaacaagt aaagtatgtt
    26161 gttaagaaag tactataaaa ttaaaaatag taatgtagaa aaaatgagca ataatcttat
    26221 tttttctaca ataattttct ataatttaaa gtaaggaaaa tttgataaat tataacctta
    26281 gttaaagaat aagagtttat taaaaaataa agtgtgtata ggggttttag ttaataaaat
    26341 attataatgt aggggagctt gtaatgatat ataaaaaaga taaagtaatg aaagttttag
    26401 aatcatcata tagggaaaat gaaaaaggga ttacagctaa ccaattatca gaaaagacag
    26461 gaattgcaag aaacaatata agtacatatt taaaccaatt attcaatgag ggtatggtta
    26521 cgaagataaa aggaagacct gtgtattata tcccagcaaa tcttaacctt aacaagaatg
    26581 aattgcttga tacacaaagt gagttgcttg gcataaaaaa aggaacaaaa gtagaaaaca
    26641 aaataaaaaa agatagtttt gaaacactaa taggtaaaga acatagttta agacctataa
    26701 tagaaaaatg caaggtcgca atgctctatc ctacaaatgg acttcatact attatctatg
    26761 gagaaacagg agtaggaaag agtatgattg ctagatatat gtacgattat tctatagata
    26821 gtggaattag aaaaaataaa gctccttttg taacatttaa ttgtgctgat tatgctaata
    26881 atgcacaatt acttatggga catatttttg gggtagaaca aggagcttat acaggtgcag
    26941 aatcttccag aaaaggtctt ttggaaattg caaatggagg gatactattt ttagatgaaa
    27001 ttcatagact accagcagaa ggtcaagaaa tgttgtttac ttttatagat aagggaacat
    27061 ttaaaagact aggagatgca acgaaagaaa gagagtctaa ggtattaata atatgtgcta
    27121 ctacagaaaa tccgacatca acactattag atacattcaa tagaagaata ccaatgaaaa
    27181 tagaaatacc atcactgaga gataggacaa tgattgaaag aatggaatta gtcaaaaatt
    27241 ttttcaagga agaatcaaaa agtatttcta tttcaataaa agtacataaa gaaataatta
    27301 aatcactatt actttatgat tgtaaaaata atgtaggtca acttcaaaat gatataaaat
    27361 tagctgttgc aaatggatac ctaagatata aaagagataa taaagaacca gtttatatag
    27421 agaaaaaata ttttgataaa tctataaatg aagtgagtga tgattataga gagaagtcta
    27481 ttaaagcaga tgaagttata acacatggaa tagaatattt tgtatttacg tcattaggag
    27541 aagaagaagt tattaactac aatgatataa atattattaa tgcagtaaat actaatctta
    27601 ataaatctgt agaaatgaat atagcagatg cttctctatt taataatgtt aagtttaaaa
    27661 gcatgtgtga atcaataaga caaattatta aagaagatga agaaatagat ataagtgaat
    27721 caatatttaa gtcaatggca atttatataa aaagcgtatt ggataaaagt agctctacaa
    27781 acaacataga tttgaatcaa attagaagaa ataacagaag tgaatttaaa acagcactaa
    27841 aaatagttgg aataattgaa aatgaatttg atttattttt atctatagaa actgtagcat
    27901 atattacatt attcatagta caagctaaac aagatgaaca aaataaaata gtgaataaca
    27961 ttaatattat agttgctatg catggagaaa ctacagcaag tagtatggta aaagcagttg
    28021 gtgatattat tggaaagtgc agtgctattt ctttcgatat gaagttagat aaaagctatg
    28081 atgaagtaat tgtagatttt aaaaatttaa ttaataatat agacaataaa gatgtattac
    28141 tatttacaga tatgggttca ttaaactctt ttgatgaaat tataaagaaa gaaaaaaagt
    28201 gtggagtaag agtaattcct atggtgacga cattaactgt gttagaagca gttcagaagg
    28261 ccaatatggg attaccttta aatgacgtat acaactctat cactaacacg agaaagtact
    28321 attttggaac taatgaaatt caaaataaag aaaatctgtc taaaacaatt attatagcat
    28381 cacatgtttc agaaggtgta gataataaaa caagaaaaat attagaagaa aagatgagta
    28441 ggtacttaga tggaatagat attatatctg tcccatataa aacagaaaaa gatttaagtt
    28501 taaatattac gaaacttaaa gaaagtagta atattgttgc agtaatcaat gaacaaagaa
    28561 ttaatattag aggaattgac tatattagca aaaaagatat agataaggat gaaaatatta
    28621 ataaattaaa aaatattata aagataagta taggttatga tgatgtagtt gaaggtttaa
    28681 agacatcctt aaaaagctct aattataata ggatatttaa agatataaaa tatgtgtctg
    28741 atgaattgtt cttagtattt aatatagaaa agaaatacga taaagtaatt ggacttatga
    28801 tgcatttagc tttcatggta gatgggttga ttggaaatac tagagaaatt gaaaagcttg
    28861 ataaagaaaa aacacttgat tatcacaaga gcctaagcaa aataaaagat atagtatctc
    28921 aattagataa aaaatataat atagaaatta atgaaaaaga gtgctatcaa atattattaa
    28981 tattagaata tgcagaaata atcgaaaaag attaccaatg agtataaaaa cttatttggt
    29041 aatttttttt agtctaaaat aatattaata ttagacacat ataaatatac ttagacaaag
    29101 tatctaagtt ttgatgataa aaatgattga aataacagtg ttttaaagtg tctaaaaaaa
    29161 ctggcataat acttgcttat attattaaat gagcaatata aataattaaa atttaaataa
    29221 aaaggagtaa ataatatgca agataaaata ttaagagata aaaaggcaaa tgacccatgg
    29281 gcaagaatga ctacaagaaa cggatttagt gcagacgaaa taatatcgtc atttcaaaaa
    29341 gctataagaa gaaatatggt ggaagaagct tgtgaatttg cttatgaact atatatatca
    29401 tcaccagaat tagaagataa gctatggaga aggatactta caatatcagt tgaagatata
    29461 ggaatggggg acccatcagc tgtaataatc ataaataatt taaatcaagt aagaaaggaa
    29521 tattcatatg cagatggaga cagaccttta tttttcatac atgcaataag atatctttgt
    29581 gcatgtgaga aagatagatc aagtgattta ttaaaaaata taattataaa aggatttgca
    29641 atgggtaaag ttccagaagt tatggacgtg gcacttgaca agcatacata tagaggatta
    29701 gaaatgggaa gggattcatt ccacttttta aatgaagcta gtatagtaat accagaaaaa
    29761 gaagtagata atgattataa agaaagatac ttaaaaatat tagaaaaata taagcaagaa
    29821 gaagttatag atacggcttt caagtttaat cattggcagt actaagaatt agagtaaata
    29881 ttaataagaa ttaaagtaaa aactgatagg aattagagta agaattggct gataaaaaat
    29941 tagaaaacaa aagaggttag atattgtagg agggtaatta tgaatatttt attagtatgt
    30001 gcaaatggag cttcaacagg tgtacttgtg gaaaagatga aaagtttttg tagtgagcat
    30061 gaaaagttaa aaactaaaac tataaatata gaggcaacat catttgaaaa tttaaaatca
    30121 tacattgaag ctaatgatac agatgtagta cttgtagcac cacaaattag gtttaaggaa
    30181 gatgatgtag ttgaagcttg taagaattac aaaatagcag ttggtttaat agatactaag
    30241 cattatggtc gtatggatgc tccttctgtt atgaagtcgg caatcgaatt atataaaaat
    30301 agataattgg ggggaataaa aatggaagca cttcaaaata atttaagaag atttcttctt
    30361 ccaatagcac aaaagattga aaaacaaagg catcttcaag ctataaaaga aggtatgata
    30421 agcattaccc caataataat tgttggttct ttaagtatgt tatttatggc attaaacaat
    30481 atgttacctg aaggaagtgc aaaaacatta ttatctgaaa atatggatac attacttatc
    30541 cctaataaat ttactatgag tttattatct atatattcag cattttttat agctcaggca
    30601 ctagctaaaa aatataatct taatcatgta gaaataggta tgacagcagt tgttgcacaa
    30661 cttgttgtat gtggacaagt agtagatggt gtattagata caagttattt agatgcacaa
    30721 ggtttatttg taagtatatt agtagcactt ttagtagttg atattactaa gtttatgaat
    30781 gataaaaatc tcgtaattag atttccaaaa gaagtaccta gtgtagtaaa taaaagtttt
    30841 agaaacttaa ctccaatgat agtatgtata atgttattta cagcaatagc agcaataact
    30901 aaaaatgtct caggtcaacc actaccagct attataatga atttcttagc accagctata
    30961 agcagtgtag ataatgtttt tgcagtaact ataatactat ttataactca attattatgg
    31021 ttctttgggc ttcatggagc agcaataact tcaagtatat ggatgccaat agcagctaca
    31081 tatatggcag aaaatgcaac actgatagca gcaggaggag acccaaaata tgtgtttaca
    31141 ataggttttt actatggttt cttgcaagta actggttcag ggataacttt gggattagtg
    31201 taccttatgt caagaagtaa gtgtaagagt tttaatagta tgggaaaagt tgtgatttta
    31261 ccatcactat ttggaataaa tgagccagta atatttggaa caccaatagt tatgaaccca
    31321 tatatgtttg taccatttgt atttggacct gtattggttg gagcacttaa ttttatggca
    31381 ttaaaagtag gattagtagg attacctatt gcagaaccac caggcttttt acctccaggg
    31441 gttgcagcat ttttgatgac attagattgg aaggcaatcg tattagtatt tgcaagtata
    31501 atacttatga ccttgatata ttatccattc tttaagataa tggagaagga agaattaaat
    31561 aaaaatgcag aattagctac aacattagat gatgatagct ttgattttta acaagtcatt
    31621 agaaggagaa ggatatggta cctgaaatgg aagaaaaagt atttagtata atatcagaag
    31681 caggagatgc taaaagtgat gtaatgatgt ctcttaagga aataaaaaaa ggtgactaca
    31741 ataaagcaaa acacctttta aattcagcct cagagaaaat acaaacagca tcaaagcatc
    31801 atttagaatt actatcaaat gctatgaata gtgaagatag tgggactgat tttttagttg
    31861 tgcactctga agaccattat tcaaatgcat tatttgctca ttctttagta tctgaacttg
    31921 tagatatatt tgagatgatg gacccaagaa ttaataagaa ttaaatttct tgtaaatagt
    31981 gcttgaagtt tggctatagt ttattaaatc aatagattta ataaatgaac tcccttttac
    32041 tttaaagtac tagtatttat aaatttattt ataaatacag taaattttta aatagaaaag
    32101 gagatattat tatggaaaaa ttttcagtag taatagctgg aggaggaagt acatataccc
    32161 cagaaatcat attaatgttg ctagataact tagataggtt accattaaga gcaatcaaat
    32221 tatatgataa tgatgaagaa agacaaaata aggtagctaa agcttgtgaa atactaataa
    32281 aggaaaagga ccctaatata gagtatttag caacaacatg ccctaaagag gcttatacag
    32341 atgtagattt ttgtttagct catataagag ttggaaaact tgaaatgaga gaactagatg
    32401 aaaagatacc tctaaaacat ggggtagttg gtcaagaaac ttgtggacca ggtggaatag
    32461 cttatggaat gaggtctata gaaggtgttc ttgaaaacat agattatatg gaaaagtatt
    32521 ctccaaattg ttggatgtta aattattcta atccagcatc aataatagcg gaagctgtta
    32581 gaaggttgag acctaattct agggtaataa atatttgtga tatgccaata ggtatggaac
    32641 ataatatagc aaggattgca ggactcaaat ctcgtaaaca tatggatata agatactttg
    32701 gattaaatca ctttggttgg tatacttcaa ttaaagataa aaaaggaaat gaacttttac
    32761 caaaattagt agaacatata aaagaatatg gttttataaa tggtgaagaa ggaatgaaaa
    32821 atgacaagaa agatagttgg tttgaaacta atttatttac taaagaaata gtaaaaacag
    32881 atttaactac aataccaagt agttacttaa agtactatct atttccagat tatgttgtaa
    32941 atcattctga tgtaaattat actcgtgcca atgaagttat tgatggaaga gaaaaagaag
    33001 tttttggttc atgtgcattg atagaaaagc aagggcattc taagggcagt gggcttaaga
    33061 caggaataca tgcagagttt atagtggatt tagctacagc tctagctttt aatacacagg
    33121 aacgtatgtt attgatagtt caaaataatg gagctataga aaatatacaa gatgatgcta
    33181 tggttgaaat tccatgtata gttggaaaag acgggtatga accattatca atagggaaaa
    33241 taccaacatt ccaaaaaggt ttaatagaac aacaattagc agttgaaaaa ttagtagttg
    33301 atgcttggat tgagaagtct tataataaat tgtggcaagc acttatgcta tctaagacag
    33361 ttcctagtgc aacagttgca aaagaattac tagatgattt aatagaagca aataaaaatt
    33421 attggccaaa acttaattaa gaataatata tttttaaagt tcctacagtt tagtacatag
    33481 cctagttaat gtatttgtag ggactttatt tatctacaaa tgagatacaa gtggcaaaca
    33541 tagtgtattt tgcagttagg aaagtaggtt tagttacttg ttagttggat taaatttagg
    33601 tacaataatt tactatcaat caatagaaat aaccagagaa gttgcataat aaaaaattta
    33661 cacttttaac aggatttatg taggtgttta tagaaataaa taaatatgta cataaataca
    33721 taattatttt taagtctaaa atacaagaat ggagagatgg ctatgaagtg gagagattat
    33781 gctgatgatg taaatttatt tgaggatttt gataaaacta tcaagcctct aactgacgaa
    33841 caaagaagaa aaaatataaa cactttaatt gcatattttt caaaagaagt tccttctaaa
    33901 atatacaatt tatcaaatga tgaaattaag cctagagata tacttagagg acttttgaat
    33961 gtatatccac caaaagaaat tgctccagag atacttaata tgttacataa tttattgttg
    34021 attgaatgtg aagaaagaga gttagtagat gtaaatgata tagaagaagt agaggaaggg
    34081 attgccatat ggagaggtaa tataacgaat ttaagagcag atgcaatagt aaatgctgca
    34141 aataacaaat tattagggtg tttacaacca ttacatctat gcgtggataa tgagatacat
    34201 tcatgcgcag gtcctagact tagagaagat tgtgacaaaa taataaaaaa acaagggcat
    34261 ttagaatata ctggagatgc taagataact agaggttatt gtttacctgc aaaatttgta
    34321 gtacatactg ttggacctat tgtatctggt ggtcagccaa gtaaagagca agaaaagcaa
    34381 ctgcttcatt gttataaatc atgtttaaat acaataaaag aaattgatga aataaaaaat
    34441 attgtttttt gtggaatttc tacaggtgta tttggttatc caaaaaaaga ggcagctaat
    34501 cttgctgtaa gtagagttag actatggcta aaagaaaatc ctgaaaaaaa tttaaaagtt
    34561 gtatttaatg tgtttactga agaagaagaa gagaaatata gaagaatatt taaataatat
    34621 tatgtaacag aatcttaaaa atacttatta tataatacaa attcattgta taagataata
    34681 atttacaagt agtaaaaatt aataaaatat aatttgtatt tttaataaat aagtaggtat
    34741 aa
//
LOCUS       DACXMZ010000013        33148 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.013, whole genome shotgun sequence.
ACCESSION   DACXMZ010000013 DACXMZ010000000
VERSION     DACXMZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.013"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            15..716
                     /locus_tag="JD016_02515"
     CDS             15..716
                     /locus_tag="JD016_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU4998503.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            940..2241
                     /locus_tag="JD016_02520"
     CDS             940..2241
                     /locus_tag="JD016_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAU4998504.1"
                     /translation="MENFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            2378..3472
                     /locus_tag="JD016_02525"
     CDS             2378..3472
                     /locus_tag="JD016_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAU4998505.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            3474..4607
                     /locus_tag="JD016_02530"
     CDS             3474..4607
                     /locus_tag="JD016_02530"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAU4998506.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDVGKFY
                     EDTKRYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIEENE"
     gene            4625..5734
                     /locus_tag="JD016_02535"
     CDS             4625..5734
                     /locus_tag="JD016_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAU4998507.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            5799..6539
                     /locus_tag="JD016_02540"
     CDS             5799..6539
                     /locus_tag="JD016_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAU4998508.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKIKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            6544..7536
                     /locus_tag="JD016_02545"
     CDS             6544..7536
                     /locus_tag="JD016_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAU4998509.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEGMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(7724..8605)
                     /locus_tag="JD016_02550"
     CDS             complement(7724..8605)
                     /locus_tag="JD016_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAU4998510.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFIRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            8878..10266
                     /locus_tag="JD016_02555"
     CDS             8878..10266
                     /locus_tag="JD016_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998511.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFTPAFSSRL
                     AGARTHTALPLPGRDLVVIQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            10288..11247
                     /locus_tag="JD016_02560"
     CDS             10288..11247
                     /locus_tag="JD016_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAU4998512.1"
                     /translation="MKMKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSIN
                     IKHCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHT
                     IMDRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHA
                     MLALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVC
                     PHCHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHG
                     DDIKKMEGHYAEVRKTQEFKERKTSYNFIETLTKEKQAGVSLG"
     gene            11307..11531
                     /locus_tag="JD016_02565"
     CDS             11307..11531
                     /locus_tag="JD016_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998513.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            11524..12036
                     /locus_tag="JD016_02570"
     CDS             11524..12036
                     /locus_tag="JD016_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAU4998514.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            12068..12922
                     /locus_tag="JD016_02575"
     CDS             12068..12922
                     /locus_tag="JD016_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HAU4998515.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            13456..14250
                     /locus_tag="JD016_02580"
     CDS             13456..14250
                     /locus_tag="JD016_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAU4998516.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            14354..15160
                     /locus_tag="JD016_02585"
     CDS             14354..15160
                     /locus_tag="JD016_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAU4998517.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIKLINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            15222..15743
                     /locus_tag="JD016_02590"
     CDS             15222..15743
                     /locus_tag="JD016_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAU4998518.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            16158..18245
                     /locus_tag="JD016_02595"
     CDS             16158..18245
                     /locus_tag="JD016_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAU4998519.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGIYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNTEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            18260..18547
                     /locus_tag="JD016_02600"
     CDS             18260..18547
                     /locus_tag="JD016_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU4998520.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            18574..19845
                     /locus_tag="JD016_02605"
     CDS             18574..19845
                     /locus_tag="JD016_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HAU4998521.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            19879..20502
                     /locus_tag="JD016_02610"
     CDS             19879..20502
                     /locus_tag="JD016_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAU4998522.1"
                     /translation="MISRRIHLMENTLSEFLDNISNELSEFIASIDETSINKACELIL
                     EAEKNHGRVHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVI
                     AISNSGETQELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKA
                     PRASILAETIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            20507..21412
                     /gene="dapA"
                     /locus_tag="JD016_02615"
     CDS             20507..21412
                     /gene="dapA"
                     /locus_tag="JD016_02615"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAU4998523.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            21518..22150
                     /locus_tag="JD016_02620"
     CDS             21518..22150
                     /locus_tag="JD016_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HAU4998524.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGLNIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNANKIREIIKNVRKI"
     gene            22628..25135
                     /locus_tag="JD016_02625"
     CDS             22628..25135
                     /locus_tag="JD016_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAU4998525.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            25477..27639
                     /locus_tag="JD016_02630"
     CDS             25477..27639
                     /locus_tag="JD016_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAU4998526.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            27644..28261
                     /locus_tag="JD016_02635"
     CDS             27644..28261
                     /locus_tag="JD016_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4998527.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            28450..28794
                     /locus_tag="JD016_02640"
     CDS             28450..28794
                     /locus_tag="JD016_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAU4998528.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            29364..30230
                     /locus_tag="JD016_02645"
     CDS             29364..30230
                     /locus_tag="JD016_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAU4998529.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWKK"
     gene            30379..30747
                     /locus_tag="JD016_02650"
     CDS             30379..30747
                     /locus_tag="JD016_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998530.1"
                     /translation="MYDDYKYNKCNKCYNDYEEMTHVHEYSESVKLAEECEDRHNHRA
                     AGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGTDKHIHLICGETT
                     VNDGHCHKFLFTTQIEAPLV"
     gene            30914..31363
                     /locus_tag="JD016_02655"
     CDS             30914..31363
                     /locus_tag="JD016_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU4998531.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNEIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(31519..31902)
                     /locus_tag="JD016_02660"
     CDS             complement(31519..31902)
                     /locus_tag="JD016_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAU4998532.1"
                     /translation="MVMLTPYLIFNGDCEKAFNFYAEAFGGGKTIFARLDSNPNNPVM
                     HASVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLP
                     HPPPHQNDGAAEVLDRYGYTWYLST"
     gene            32158..33075
                     /locus_tag="JD016_02665"
     CDS             32158..33075
                     /locus_tag="JD016_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent protease"
                     /protein_id="HAU4998533.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
BASE COUNT        12919 a         3601 c         5876 g        10752 t
ORIGIN      
        1 ttaagaggtg aatcatgtta agaaatcaac cattatatat acaagttgta gatatattaa
       61 agcaaaaaat attaaataac gaatataaaa ttgatgaaaa tattccaaca gaaagggagt
      121 tagagcaaga gtttggagtc agtaaaatta ctattagaaa agctattgag atattagaat
      181 cagaagggta tgtagagaaa aaaagtggaa aagggacaaa agtaataagt aatagtcttt
      241 ttaataagct atcaaaagca gagtcttttt caagtatact tttatctaaa ggtcataagt
      301 tatctaaaaa gactatttca attgaaaaaa taataaatga tacaaattca caagtattta
      361 gtatatttgg agaaaaatgt agcaagatta ctagaatgta ttacttagat agtaagccat
      421 atatattttt tactcattat attccttttg atgaaaatct aactgttgaa agttttaata
      481 atgaaagttc tttatatatg tacttatata ggagaaatat aaatataact agatttgaag
      541 atgaattttt tatagaagaa tcagaactag aggtatcaaa ggctctaaat ctagctaaag
      601 acatattatt aggaagaaaa agaaagacct atgatgagaa tgataatata gttgagctat
      661 catttgcaag atataataca gaagtacaaa actatgtaat taaatacgaa atataataag
      721 aatcctgagt ttattgaaat tataatatgc tcaggatttt tgtttagttg taaaaacaaa
      781 aaatattata acattataat gtttacaagc aaacattata atgttataat atttttaata
      841 aacaagtgat attatcaatt acataaaaat tagtttatgt tatgaaatcg ttattgcatt
      901 ataaataaag gataatatca ataatacagg gggataacta tggaaaattt cgcaagtttt
      961 ttagagcgca agattatgcc tgtagcagcg aagataagta atcagagaca tatgagagct
     1021 gtaagagcag gtataatagc aacattacca ctaacaatag tggggtcatt tttcacaata
     1081 ttattaaaca tacctattga tgcttatatg gattttatag ctccatttaa agcaatatta
     1141 gatataccat ttagatatac tgtaggattt ttatctctat atgctacttt tgggatagca
     1201 tcatcacttg ctaaaagcta cgatttagat acaacaggtg tcggtatgct tgcagttatg
     1261 gcatttttag tttctacaat agttccaact caagtagttg aaccagtagg tggagttata
     1321 gaagctggta gatggatgga tatggcaaaa cttaattcgc catcattatt tggagctatc
     1381 gtaacatcat taattacagt tgaaatatat cgttttatga aggaaaagaa tattacaatt
     1441 aagatgccag cgggagttcc agtagaggtt tcaaattcat ttactgcatt attaccgaca
     1501 gcagtaattt taatgttatt ctgggtaata agacatattt taggatttga tataagtgtt
     1561 gcactaagcg ctatgttatc acctttaaaa ggattcttat caggaaatag tttaggtggt
     1621 gggttactga cagtattcct aataacttta ttctgggtac ttggaataca tggtccagct
     1681 ataatgggac cagtaataag acctatatgg gacatgtcta tagcagaaaa tataactgca
     1741 ttcacagaag gagtatcagc tcatgcaatg ccaaacatat ttacagaaca atttttacag
     1801 tggtatgtat ggataggggg agcaggggct actcttgcat tagttgtaat gtttatgttt
     1861 tctaagtcac aatatctaaa aagtttaggt agattaggat ttttaccagg gttattcaac
     1921 ataaatgaac ctataatatt tggagcacca atagttatga atccaatact agggatacca
     1981 tttataattg gacctataat aatgacaatt ctatcatatg ttcttactat tacaggggtt
     2041 atacctatga tggtagcaag attaccattt gcaatgccag caccaatagc agcagtaatg
     2101 agtactaact ggagtatact agcaggtatc ttagtattaa tcaactttgt gatatcattt
     2161 gctgtttatt atccattctt caaagttttt gaaaaacaac aattacaaag agaacaagaa
     2221 gaaatgagtg aaaatttata aaaatacata gatatagagg ttttgtacta tataaaatat
     2281 agaaaaagct tataagttat taagggtgaa ggtaggagga atgacctttg cctttaattt
     2341 tatatatttt aaataatgta atttgaggag gagaaagatg gaaagaagat taggaatatc
     2401 agtatatcca gagcatagta acatggaaga tgataaggca tatataaaaa tggcttcaaa
     2461 atacggattt aaaagaatat ttatgtgcat gctttcaatt acaagaccta aggaagaagt
     2521 taaaaaacat tttaaagaga ttataaccta tgcaagagaa ttaggatttg aagtaatcct
     2581 tgatatagct ccaaacatat ttgatatatt acaaatatct tatgatgatt taacattttt
     2641 tagtgagtta aatgctagtg gaataaggct tgactatgga tatgatggaa gtaaagaagc
     2701 tatgatttct ttcaatccat atgatttaaa agtggaacta aatatgagta atgatgtagc
     2761 atacttggat aatataatta cttacaagcc aaatgtaaat aatatatatg gttgtcacaa
     2821 tttttatcct caaaggggta caggcttgcc atatgatttt tttgtaagat gttcaaatag
     2881 atttaaaaag tatggaataa gaactgctgc ttttgttaat tcaaaatacg caacaatagg
     2941 gccttggaat ataaatgatg ggctgtgtac gcttgaaatg catagaaact tagatattgc
     3001 tacagcaacc aagcacttat tcgcaacagg attgattgat gatgtaattg tagggaatgc
     3061 atatgcaagt gaagatgaat taaaaagaat gtctgaagta aataaatatc aagttgtatt
     3121 taatgtagta actgaagaaa aaacttctga agtagaaaag acaataatgg caaatgagca
     3181 acactttaga agaggggata taaccaacca agtagcaagg tctacagaag taagaaaaaa
     3241 atatgctaaa gaaaatttcc ctaagcatga taatacgacc atctttaaaa gaggagatgt
     3301 agtgattggt aatgatgact ttggaaagta taaaggggaa ttacagttga tacttcaaga
     3361 ttgtgcagat gaaagaaaaa ataaggtggg tagaataaag gaagacgaaa taatactttt
     3421 agactttatt gaaccttgga gtaaatttaa gattatttta gagggagagt aaagtgaaga
     3481 tagacatatt aataaaatgt ggaaaaacag tagataaaag tataattgat atagctatat
     3541 tagacgataa aataatagaa gtaaaaaatc agatagatga ttctaagtat caggcaaaac
     3601 aaattataaa cttagatgga gagaaattta ttagtgctgg ttggatagat atacatacgc
     3661 actgctacga aagactagac ctttataagg attatccaga tgaggttgga ataaaaagcg
     3721 gtgttactac tgtagtagat gcaggaacaa caggagcttt agatgtaggt aaattttatg
     3781 aagatactaa aagatataag acaaatgtat atgctttaat aaatattgct aagcagggaa
     3841 taacatctca agatgaactt tcaagtatga tgaatattga tgaatatgaa cttaaaagag
     3901 cagttaaaaa atacaaagat tttgttgttg gaatcaaagc tagaatgagt aaaagtgttg
     3961 taatttcaaa tgatgttgaa cctttaaaag tagctaaaag aataaaaaat gaacttaatc
     4021 tacctatgat ggttcacttt gggagttctc ctccaacgat agaggatata tttgactata
     4081 tggaaaaagg agatatatta acccatatat ataatggaaa gccaaatgga atacttagag
     4141 gcaatgaggt taaaaaagaa atcatagaag ctagagaaag aggtataatc cttgatgtag
     4201 gtcatggaac cgaaagtttt agtatggata ttgcaatgaa atctaaggat gcaggaattt
     4261 ttccagacac tataagtaca gatatatata ttaaaaatag aataaatggt ccagtgtata
     4321 atctatctac aacaatggaa aagtttatat atatgggcta ttcacttgaa gatataatag
     4381 ataaagttac taaaaatgca gcagatgcaa taagtttaaa aaataaaggt ttaataaaag
     4441 aaggatatga tgctgattta actatatttg atgtggttaa tgaagaaaaa gaacttcaag
     4501 attctttgaa taagtcagta atcacaagta cgtctataaa gccaagagca gtagtagtca
     4561 atggagaata tttaaatata ggtaagtcta tagaagagaa tgagtagaag ggagaaacta
     4621 ttgtatgagt agtatatttg aaaaatactc tgtaagacgt gttataaatg cttcaggcaa
     4681 aatgactata ctaggagtat caacagtaga taaggaagtt attgaagcaa taggtgaagg
     4741 gtgtaaaaat ttctttataa tagaagaact tataaataaa acaggtgaat atatagcaaa
     4801 tttattaggg tgtgaaagtg cactaatagt ttctagtgca tctgctggga ttgcacagag
     4861 tgtgggagca atgatagcaa aagatgatat gagtctagtt tacaatgtaa ataatcctaa
     4921 gaaacaagtt aaaagggaaa taataattcc aaagggacat aatgttgatt atggagttcc
     4981 agtagaagta atgataagac ttggaggagg agaagttgtt gaggccggtt atgctaatat
     5041 gtgtaacaag gaacatataa taagagagat aaatgaaaat acagcagcaa ttatgtatat
     5101 taaatctcat cattgtgttc aaaagagtat gctaagtgta ttagatgcag tacaagttgc
     5161 taaagaatat aatttacctt tgatagtaga tgcagcagca gaagaagatt taaatgtata
     5221 ttatgaattg ggagcagacc tagtgatata cagtggtgct aaggctatag agggtcctag
     5281 ctcagggtta gtaataggta agaaaaaata catagataat gtaaagcttc aatcaaaggg
     5341 tataggaaga gctatgaaaa taggaaaaga aaatattgta ggtctaacat gtgcaattga
     5401 gagatatata agtaatgata aagtaacttt aaaagaaatg gaggacaagc taaatccatt
     5461 tatagacaag ataaatacta taaagggagt gagtgctagt ataactaggg attcagcagg
     5521 tagagaaata cttcgtggag aaattgactt taatgaagat ataataaata agtcaacaca
     5581 acaaattata aatgaactta gaagtggaga gatagctata tacactagag actataaggc
     5641 taatgaaggc aagattgaga ttgatataag aagtgtaact ggaagtgatt tagatactat
     5701 ttataatagg ataaaaaata tagtagaaag ttagagtgta gagtaagtga cattataatg
     5761 atttaataaa ttaaatttta ccaatggagg ggataaatat gaattgtata ccacaatatt
     5821 ataaaaatag agtttgttta aatgttcttg caggaagcat aaaaaatgct aaagaagtat
     5881 atgatgcttg tgacgggcat gtattgatag gagtactatc aaagaattac agcactgtag
     5941 aagcagctat tgaagatatg aaaaaatact caaaggaaat agataatgca atatctgtgg
     6001 ggcttggagc tggagaccca aatcagtcaa atatggtatc tcagatttct aaagaacttc
     6061 agccacaaca tataaatcaa gtgtttacag gtgtggctac gtctagagca ttgcttggtc
     6121 aaaatgatag tataataaat ggattagttt ctccaactgg taaaccagga tttgtaaaaa
     6181 tatccacagg tccattaagt tcaaaagaaa aagatggtat agttcctata gaaacagcaa
     6241 tagctatgat gaaagatatg ggaggaagct caattaagtt ttttccaatg ggaggactta
     6301 aaataaagga tgaatatata gaagttgcaa aagcttgtgc aaagcataat ttttatttag
     6361 aaccaacagg aggtatagac ttagataact tcaaagagat agttcaaatt gcacttgatg
     6421 ctggtgtaga aaaagttata cctcatgttt atacatcaat aattgataaa gaaacaggtg
     6481 aaacgaaagt ggaagatata ggagtacttt ataaaataat gaaagaatta ttgggataga
     6541 tttatgaata tattagcttt tggtgaaata atgatgagac tttcagtacc tgactacaag
     6601 ttcttaacac aaaccaatga actaaattat ataattacag gcacgggatt aaatatatta
     6661 tctggactta aaaactttgg atacaatacc tatatgttga caaagttgcc taataataat
     6721 gtaggtaaag catcaagtgc aaatataaga aagcttggag taaaagatga ctttatcacg
     6781 tatggaggga atcatatagg agtgtatttt cttgaaaatg ggtatggaga aagaccttct
     6841 gaagtgactt atttgaatag gttaaatagt tcattttgtg agagtaaatt acaagattat
     6901 gatattgata aatgtttaga aggaatggat atagtccata tttgtggtat tgctatgcaa
     6961 cttacagatt cagttaaaga gattgcttta gaactagcta gaaaatctta tgaaaagggg
     7021 ataaaagtat tctttgattt taattttaga caatcattaa atccagaaag gacttatgat
     7081 gatttagtaa atgattataa gaaaatacta ccatattgtc atgggctgtt tgctagttat
     7141 agagatataa ataagatact taatatagat agtctaaata taaaatctag ttcagaattg
     7201 attaaaaaag agtatgatat agatattgta gctggaacta tgagaagatt tgatgatatg
     7261 aataatagat ttataaaggg atatatatca aatgatgatg gttattttga aagtagggag
     7321 tataagttag aaatatatga cagggttggt gccggagatg gatatgtagc aggaattata
     7381 tattcatatt tggaaaatat gaagagtgaa gatatagttg agtttggggt tgctagttcg
     7441 gtgttagctc atacaactta tggggataat tctttagtaa gcacaggtca tgtacttaga
     7501 ctgatgaaca atgaaaaaat agatatattg agataataca tttgtatata ataaaatttt
     7561 tatctttttt agacacattt ttgacttgct tatatttgtt aaacagagtt atttttaacg
     7621 attataaata ttaaattaaa agttaaataa taaaatagta gaaaatagca ttaactatag
     7681 aaaaaatggt taatgctatt tttatcgtta atattgatta attttatatt atagaaatga
     7741 acttttctaa tgctggattt gtattttttt tattccatac aactacaact tcaattctat
     7801 tttctacttc ttttataggt actattgcaa tatcacgttc tatcattgta tctgtaacac
     7861 aaaatgaagg taaaatggat attcccatgt tagaagatac caatgattca atagtattaa
     7921 gattatttga acgttgtacc acattaggta caaaccctgc ttcacgatat ctattctgta
     7981 tcattgactt ttgtccatac tcatcccctg tttcttttac aaaaataaat ttatcatgtt
     8041 gtaattcaat taaatcgata gaatcctcat tgtaaagtgg atgaccagga ggtaaaagag
     8101 ctaccaaaga atatctttct acagttttat attcaaaatc ttttaatggt tgattttctg
     8161 gattaataac aaaagcgagg tccatagaat tattttttac taagtttaaa agttcggtta
     8221 tttcattagt acaaatatcc atagatatat tagggtatgt attagaaaat tcttttaagt
     8281 attgttgaag tttgtttttt tcatatccta ttaaggttcc aatgctcaaa gttccaaaaa
     8341 aaccatgtgc aaattgttgt gtttttgcaa tagcatcatt gatatttttt attatagtac
     8401 gtgcctcaga taaaaaaact tttccagctg gtgttagttc aacatgacgt ttacttctta
     8461 taaatagagt tacatttatt tgttcttcta aagcttttat ttgctgtgta acagcagttt
     8521 gagaaatata aaatttttct gctgttttgg taaaacttag attttctgct acagatataa
     8581 aatattctaa ttgtttagta ttcaaataat cacccctatt taaacttgat tgtttgtaaa
     8641 ccttatttat ctataattaa agcttattat agtataagaa aatttacttt taaagaatat
     8701 tagaaaatat ttgtataaaa atatatgttt taagtatatc tttacattag attttataaa
     8761 tttattaata agaataactt atatattcca atgaaaacga tattggattt aagtaagttt
     8821 gtctcataaa atgaaagtat atcaattgat aatactttaa ataagaaggg agaaaaaatg
     8881 gaagataaat tttatgcaaa aggcaacgga aataacggat atattaaaaa tcttgaagtt
     8941 tgttccttta ataacttaga tggaacttgt ggaatgtttc aaatggctct gtacaaaaga
     9001 gatgaaaaat actatttata tggatgctgt tttggaggaa ataaaaaaaa tggagtaatg
     9061 attagcgata ttacagaccc ttataatcca caatttataa aacattttca aatgttagac
     9121 cctaaagagt atcctacaac aacaactccc aaaattcaaa tagcagatga tttaatgata
     9181 gtagcaatga gttgtggaag tggaccagga gcacttgttg accaagctaa attagcaaat
     9241 attaagtgtg aagcaggaat tagaatatac agtttaaaag aagacccttt aaatcctaag
     9301 tttttaggat attgggattg tggcttaaag catgtaatgg gtgttcatag attcatgtac
     9361 aatggtggaa gatatgtaca tttatcaagt gattgtgttg gctttgaagg tctgatttat
     9421 agggtcatag atataataaa tcctactaat ccagtggaaa taggtaaatg gtggagacca
     9481 gaccaatatg cagatggata tccaaataga acttttgatg caggagcacc tcattgccca
     9541 gaatttatgg ataaaggatg gcttcatgga cctccatttg taagagacgg aaaagcatat
     9601 tgtggttatg gaggagctgg tttagttgta ttagatgttg aagatttaac aagaccaaga
     9661 tgcttaggtg aattgccatt tacgcctgca ttttctagta gacttgcagg tgcaagaact
     9721 catacagcat taccattgcc aggaagagat ttagtcgtta ttcaaaatga gggagaaaga
     9781 ttccagttct ttaaaccaga taacattaca gatgttcaag ctatgaataa tatacatatg
     9841 gttgatgtta gtgacccaac aaaaccaaca ttaattgctc aatttccata tcctgaagtt
     9901 ccaaaagatt tcccttatcc taactttaat gttgcgggat taggaaaacc agggccattt
     9961 ggtccacata atcttcatga accaatggat aataagccat ggttagagca aagaggagat
    10021 agagtatatt gctgttattt ccatgcaggg ctaagggttt atgatgtatc agacccatat
    10081 tatatcaaag agctagcata ttttatacca ccaaatccaa ataaaacacc agaagaatct
    10141 tatttcccag gattcccagg accacgcttg gcagtaacag aagatcttat cgttgatgat
    10201 agaggctaca tcatcataga tgctttagat gatggattct atatattaaa aatgaaagaa
    10261 gattaaataa atactaaaga gggagagttg aaaatgaaaa tactaggtat ttcttttgga
    10321 acaaaaaatg gaaataatga cacaatgtgt aaagttgcac ttaaaggtgc caaagaagca
    10381 ggggctgaag tagaatttat tcaaatgtca tctattaaca ttaaacattg tactggatgt
    10441 tgtgcgtgtg ttaaaacttt attatctggg aaaggtagta tgtgtgtatt aaaagatgat
    10501 tttgaatggt tgcttgacaa aatgaaagat gcagatggta tagtagtttc agaccctatt
    10561 tttgaagaag gggcatcagg attattccat actataatgg accgttttgg acccagagca
    10621 gatagaggaa ataacattat aggaacaaaa gttgcagaag ctataggagg aaaaattcca
    10681 gattcaagaa tgcttaatga taaagtaata tcatttatgg gaataggagg ttctgactgg
    10741 ggtacaagag ttcaatgtga acatgctatg ctagctctca taccaatgtg gaaagttata
    10801 gataatgctt ggttcccatg ggcaaaggaa ttagtaatgg atgatacaag gcttgttcaa
    10861 gttcatcaaa ttggtttaaa tatagttgaa gcagcaaaag attttgaaaa agcttcatat
    10921 caaggagaag aaggtgtatg tccacattgc cacaatagat tattctacct tgagccaggt
    10981 actaaaaagg caatttgtgc tctatgtggt atagttggag agttagatac tataagtgga
    11041 aaaactatat ttagttttcc agaagagcaa ttaggtcatg ctcatgatac tttaccaggt
    11101 aagtttatac atggtgatga tattaagaaa atggaaggtc attatgcaga agttcgtaaa
    11161 acacaagaat ttaaagaaag aaaaacatct tataatttta tagagacatt aactaaagaa
    11221 aagcaagctg gagtttcatt aggataataa ttaaaatact aagactcttt tttaagggat
    11281 gttagatgtt ataggaggct tattgtatga atgaaaagcc aagttttaaa caaattgaag
    11341 ttattttagc agttattttt ttggtactta catattacaa ggttagatta actccagcat
    11401 ttattgttat atttatacca ctatatttaa gttattttgc agtatctatg attgtaaatc
    11461 aaatagcaat taagctattg gagataaggc aagctaggga agctactaat tacaatatag
    11521 gaaatggata gaaagaaaaa agaaatatta acactgtcta tggggattgc tttactacct
    11581 cctttatggg cagtattagc accttatata ggaataaaaa ctggagctgt agcactaatc
    11641 tgtgcaggat tatatgtaac taatggtaat aaacaaaaag atggattaaa aataatgttt
    11701 ggtttttggt gtggagattt atgggcagtg ttagccattt taattatggg atatatgaat
    11761 ttcaatcaaa acctagaatt atttttaaca cttagtatat taggtttttt tgcagttgta
    11821 attgcatcct tatttgaaaa aataattttc ctaccatcat ggctttgtgg ttgggctata
    11881 ggtctaacta ttatgacagg tgaaaatatt attaatttac aagatatttg tattcaaatt
    11941 gctgtagcta tggcagtagg agtatggtac gtgggtgcag gtgtagattt gtttacaata
    12001 tatattttga gtaaagatag gaaaaaggga aattgaaagc atgtgaatat actttgatta
    12061 taggagaatg gctatgggtt ttgaatttaa aataatgaga agtttaatat atgtaggact
    12121 tgccaaggaa gaatatagac ctaagctaat ggactggtta tatcgtcacc atattccaga
    12181 tagtattagc acttttggac catattgtac taaatatgcc ttttatcaag catatcctac
    12241 accaaatgaa ggtgagcgtt ttggtgcacg taagatgcaa ctaacagaac attattggct
    12301 tgtagatgaa catatgcctg agatggcaaa tagaattatg acagaatata tgcctatgga
    12361 tgttctacgt tggcaagggt gtataccaga tgtagaaaat aaaagggttc atgaaaatgc
    12421 agaaagtgga gatgcaggac gtgcagtagg tggagataat ggatgtccac catttatatt
    12481 tgcctttgtt ccaataaact gggaagaaga ctttagagga aaaggacgta ctgtacaaga
    12541 tggaccaaac tatcgttggc aatttatgat taagtatcca gatggtatct ctaaagaaga
    12601 aggagaaaaa tggttctatg atgaggtagt gccatatttt acaaactgtt gctatgttaa
    12661 tcgttttgtc agtagtaaaa taatgattaa ttatggagca actgcttttg accgtgtgtc
    12721 agaactatgg tttgaagggg aagaagaatg gtataaagct gtggttgaag agacaaagtc
    12781 gtttattaaa aaaccagaat gggcacaaga agaggagttc ccatatttaa aaccacaatt
    12841 caatatcgca tcagtattct taggtgatat agcaactatg gatgcatact cacagtatcg
    12901 tggatatata ccaatgagat aaaaattata gtttgtgata agtagagaag gcatttactc
    12961 ttactggtag aagaagactt attgtatcct taaaagtagg tgaatatata gttttatatt
    13021 taaatttata taaaactaag actaataaat atgaaacaat agaattagaa taatttgata
    13081 atatttgtta aaagagcatt tagaatgtgt ttaaagatta agatattcct tttatggtag
    13141 cagatagttg aaataataaa actgttatca tgaaaggaat atttttatgt ataggaaatt
    13201 tttaaaaata aaagttaaat atgtttaaga acatcttaaa gtgaaagaat cattgttaga
    13261 aatatgtaga caatatagtc tttataacgt atcatattaa caagtttaca cttggattat
    13321 gaaataaaaa gagtaattcc aaaggttgta aatactatgc tgactttgat acttcaaagt
    13381 ttaggtgatt ttgtaattgt taatagaaat acgtttaatg agcataaaga atttatctaa
    13441 taataggaga atataatgta tacaattgga caagtatcta aatttttagg aatatctaga
    13501 gatacactaa aattttatga ggacaaagga ttagtaaatc ctaagaaaaa tgatgaaaat
    13561 ggatatagaa tatataatca ggttgatatc tatgatattg caacaataaa cttttataga
    13621 gaaattgata tagaaattaa aaagattcaa gaaataagaa aaagtaagag tataaacaat
    13681 ttagaactct tactagaaga gaaggaacaa atcatactaa aagagataga atataaaaag
    13741 cttctcttaa aaaaggttca gttagtcaaa gaagattgtg acaaaatcaa aacatatttt
    13801 ggaaaataca caataaaaga aatgaagcct ttggaagtaa aaggtaaaat aaaacatgtt
    13861 actaactatg aggaatatga agccttaaaa gagaatacaa acaacttaaa aaaagcagtt
    13921 acattaactg atttaagaag agtaataaat tttaatgaag aaggtataat agaagaaaaa
    13981 tttataattg taagggaagt agatgactac aacaaaatca tagaaggtga agttctatct
    14041 catcccaatt gtatttatac tattattgaa gatggcagat ggtcaactgg aggagaaaat
    14101 attgataata aagtggaaga cagattaaga gaaatagcaa tagaaaatga atgtgaatta
    14161 cttggaatag tctatgttaa tatattgctt actacttatg aagaagggat tgagcgtata
    14221 tttttagaga tttatgcacc tataaaataa tataaaatca ttgacctggg acttactcat
    14281 aggtttaaac tcatgatata agcagttata acttatatag caattaaata ttaatttgaa
    14341 atgaggatta cttatgagaa cagagaaatc attaaaaagg cctatagcca gttttattat
    14401 tctaacaaat ataatttttt taccactttt tttattggta gtagtgacta taatgttaaa
    14461 acttccaaca gtgatatctg acattgcgtt gtgtatatca gcgtggtcat ccacttttgc
    14521 atttatgata ttatttaaaa aaatttatcc aggtaagaaa tttttagtac acgtaaaaga
    14581 tagatttaga aataaactta aattttcaac agtatctata ataattagta tacaagtcct
    14641 aatagctgta gtagttatat ttatattagc atctaaaaat catggaataa tgcctaactt
    14701 tacagtttca tcatggggag catttatata tttattttta aaaaatttat ttgcaggatc
    14761 attaggtgag gaattaggtt ggagaggatt tgttcaaaat catcttcaga aaagacattc
    14821 tcctttaaaa gcatccataa tagtaggttt ttggtggggg atgtggcatt taccaatatg
    14881 gtttacaaca ggattcaaag ggcttgattt aataaaatat attatatttt ttatgattgc
    14941 aattatatca atcagtatta taatgacaac tttttataat ctaaataaaa ctttgataat
    15001 acctattgta atacatcaac tatttaattt tttaataggg ataattaatg gagacttaat
    15061 aaagcttata aattactatg caatgttata tttaatattt gcaattgcat taatagttat
    15121 aaatccagga aatgtattat ataaaaatat tagagagtaa tatatatctt tatggtaaga
    15181 tataaagaaa ttattagaag aacatccaag gaggaataaa catggtttta ctgattgttg
    15241 atgcacaaaa gttaataaca aacgagaggt tatataaatt taatgaattt gtagtaaatg
    15301 tagaaaattt aattgataca gctagaaaaa ataatataga agtaatatat gtacgtcatg
    15361 atgatggagt tgaaaatgaa ttgactaaag gtaaaaatgg ctttgaaata tacgaaaaat
    15421 ttaaaccatg taatggagaa aaaatatttg ataaaaaagt aaatagtgct tttaaagaaa
    15481 ctggtctatt agaataccta acaaataatg gtgaaaaaga tattattatt gctggtctcc
    15541 aaacagatta ttgtattgat gccactataa aatgtggatt tgaacatgga tttaatataa
    15601 tagttccagc ttattcaaat acaactgttg acaatttttt tatgtcagca gaacaatcat
    15661 ataaatacta caatgaattt atatggaatg aaagatatgc gaagtgtatt tcattagagg
    15721 aaacacttag aaaaatgaaa taaaaataga gtatgaattg tatatgtaca taagttgatt
    15781 tcataaaaaa ggatgttaaa ttccaagtta aaaattagat taaatatatt atattataga
    15841 taaggcacat caattttatt ttaaattaat gtgcttttta attgtagtaa tttatttata
    15901 atcaatatga tttttattat ttaaatcaat ttttcttgag tttctttgtc taaagaaact
    15961 ttttttattt ataaaatcta cagttatatt atagtatcta ttttagatga gtttaatttt
    16021 tgtatcaatc taaaacaaaa gttatactta aatgaaattg aaaacgataa aattctcaaa
    16081 taaaataagt attgtggagg tgatgaaaga atccaatgat aatatgaaga ctaatcttaa
    16141 taagaaagta ggggttaatg gtagtggaca aagaaattgt atcaattctc cagattttat
    16201 taaatagcac attgattaca actaatggat tacaagagga gtctaattca tcaaagagac
    16261 aaattactta tagaataaat aaaatcaatg acatgcttaa agtaaaaaat gtaccactta
    16321 tatgtttaag agccgacaaa gatattatcg ttagaaaaga aacaaaaaaa gcaattaaag
    16381 aaattttaga aaaaaactat tctaagaata cttattattt tagtaagaca gaaagacttt
    16441 tatatatgta tttaatgctt tttattaaca tggaggatat ttcaattaat cattttataa
    16501 attctataaa agtaagtagg tctactgtaa atcttgattt caaggatttg ataccagagc
    16561 ttgaaaagaa gaacattaaa gttaagaaca atcgtataaa tggatattac atagttggca
    16621 atgagatgga aattaggaga gtacttatta aaaacattat agaaactttg tctaatgaga
    16681 aaagttcaag agtatttgat atttttatta aagaatataa tttagatagt tttgaagagg
    16741 ctaaaaaaat tatattaaag ctaataaaaa aatatgaaat tacttttgtt gaagatagat
    16801 taatagaatt tatatatata tttatctttc taaaagcaag gatgtattca actagaatag
    16861 ttactcagga aaatgtagat attcctaata tttcagttat gagttcaatg aaggagtata
    16921 aatttgctaa ggaattatta gaagtatatg ggagtgaaga taagataaag cctcacaata
    16981 taatgtatat tagtgcttgg attttaggta tatcagtagg aaattatgaa gaagatacag
    17041 aagataagga tgtaatttca aaaattgtaa ataaaatgat gacaaagttt ggatatttaa
    17101 gcggaattta ctatataagc caagaaaaaa tatttaaaca attatattct cattttagac
    17161 ctgcctacta tagactttta tttaaattac ctatttataa tccgatttgt gaaaaagtaa
    17221 aagaagaata taaattagtt tatagaattg taagtaatgc aatgaaagag ttttgcggat
    17281 tatttggaga agaaattcag gaagaagagt tagcatatct tacaatgcac tttgcaacac
    17341 ttttttcaaa taaaaaagaa tttgatggtt ttagaaaaag aatagcatta atagtttgtt
    17401 caaatggcgt tggtagttca gctattttac atgcagaact tacaaactta tttcctgact
    17461 tacactttat accaatgtcg gaatcagttg gattagaaaa tataagtgaa cctgtagata
    17521 ttgtatttac tactaattat aacacagagg gtttaaaaac agatgtacca gtaattaaag
    17581 taagccctgt tatgaccaca aaagaaaaat ataagattac acgtgaagtg tatattcaat
    17641 taggtgatat ttttttaaga caacctaaaa tagatgaggt tatggatata ataaaaaaat
    17701 atgcaaatgt aacctctgaa agtattcttt caagtgagct attagcttat tttacacaga
    17761 tagagaattt tacatctaag gaaggagagg ggcctatgct aagtgagatt actaatgaaa
    17821 cactagtaaa aataaaggta aaagctaaga attgggagga agctattaga cagtcagctt
    17881 ctgtattagt tgaaaataac aaagtaactg aagggtatgt agatgctatg attaactcag
    17941 caaaagcatc tggaccatac atagtaatta caaaacatgt tgcacttcca catgctagac
    18001 cagaagctgg gtcaaaagag attgcaatag gcattgctac tttagatagt cctattgagt
    18061 ttgggaataa cgaaaacgac cctgtaaaat atgtattttg tctaagtgca atagacaata
    18121 atagccattt acgtgctatg tctgagctag ttgaactatt ggaagaagat gagtttttta
    18181 aggttttaga tagtgctaaa gaagctaaag aaattattga ttttataaaa acacacgaat
    18241 tataaaggag gatttaatta tgtataaaat tttaatcgca tgtagagcag gagttggctc
    18301 aagtttaatg ttaaaaataa agactcaaca aattattaag gaaaataact ttccaattga
    18361 agtagaacat ggttcattgg attcgctaaa tggattcaat ggagatgctg tagaaacatt
    18421 aattgatgtt gctgaagaat taaaaaataa aaatttgaag tttgatataa ttgggattca
    18481 caatattgtt gaccgtaatg aaataaagac agcactagaa aaattccttg cttcaaaaga
    18541 gggataaatt taagatgata aggaggaata tttatggagt ttattattag tttgctgagt
    18601 acacctgctg ttttgcttgg cgtagttgca tgtataggat tgattcttca aaagaaatca
    18661 gctgttgaag tatttacagg ctcttcaaaa acacttatcg gttttttgat atttggtatt
    18721 ggtgcctctg ctatgacagg agcacttcaa aatttcaata aactgttcca acatggcttt
    18781 ggaattactg gtgttatagc ttcaccagaa gcagctacag cacttgcaca aggtacatat
    18841 ggttttgcag tttcatgtac attaattctt ggattcatat taaatttagt atttgcacgt
    18901 attacaaagt ttaagaatat tttctttaca acaggtcata gtttgttctt ttcttgtgta
    18961 ttagttttaa ttatgaaagc acatggattt gataatacaa tcactatttt aataggagga
    19021 actatcttag ggtttatgtc tgctgcacta cctcaattct gtcaaccatt tatgcgtgaa
    19081 ttaacaggtg gcgacgaaca agctattggt cactttaaca tgattggtta tggtttatca
    19141 ggttatattg ggcgtttatt ttcaaaacat aaagatgata caacagagac tatagaattt
    19201 cctaagtggt tatcaatatt cagagacttc ttaatgggtc tatccatagt tatgttaatc
    19261 ttattttata ttgcaacatt aaaagctggt caagcattta ctcaagaaat tgctggttca
    19321 acacattggt tggtttttcc gcttatagag gcatttacat ttgtggcagg tatgtcaata
    19381 ttgatgtcag gtgttcgtat gttcttagct gaaataactg cagcatttgt aatcatttca
    19441 gaaaagtata ttccaggttc acgtcctgcg ctagatgttc caaccgtatt tccatatgca
    19501 ccaaatgcag ttattattgg gtttatatca gcatatgctg ctggtctatt agcaattgct
    19561 attatggcat catttcctag tatatttcct gtagtaatta ttccagctgc tcacatatgt
    19621 ttcttctcag gaggtacagc tgcaatattt ggtaatactt caggaggatg gagaggggct
    19681 attgctgggt catttgttgt ggggttatta cttgcatttt taccagttat actatatcca
    19741 gtttatggca ctcttggtat agaaggagca actttcccta atattgatta caatgtagtt
    19801 ggttcgatat tacataatat tttacaattt ataaaaccta tttaaactaa attatataaa
    19861 agtatagtca tagttactat gatttcaagg aggatacatc ttatggaaaa tacattatct
    19921 gaatttttag ataatatatc taatgaattg tcagaattta ttgcctcaat agatgagaca
    19981 tctataaata aagcatgcga attaattctt gaagctgaaa aaaatcacgg acgtgtacat
    20041 gtgactggta ttggcaaacc aggacatgta tcgggatata tttcatcatt gctttcatct
    20101 actggaacgt cagcatatat attgcatggt acagaagcag tacatgggtc aagtggtcaa
    20161 gtagttgaag gagatgttgt tattgcaata tcaaatagtg gtgaaactca agagttaaaa
    20221 gcaactctta agactctaaa agtaaatgga gcaaagataa ttggtgtaag tggtaatgag
    20281 agttcatttt taaaaaatat ttctgatata ttcctgtttg caggtgttaa gcaggaagga
    20341 gattgtctta ataaagctcc tcgtgcttca attttagctg aaacaataat attacaatca
    20401 ttaagtgttg tattacaata tgctaaaggt cttaatacac agcagtattt gaagtggcat
    20461 ccagcaggaa gtcttggaaa gtctattaga gagggaatat aattttatga aatataaagg
    20521 gattatatgt gcaatgataa caccatttga tgaaaatcaa aatattaatt cacaagcaac
    20581 atgccaatta attgattatt tgattgaaaa gggaatttat ggattattta ttttgggcac
    20641 taatggtgaa tgtcatgtat taacagatga tgaaaaagtt gaatttgcaa agattgttat
    20701 taatcataca aataatcgtg tacctgtttt tgttggaaca gggggtaatt caactcgtga
    20761 agttgtagat ttatctaaga gaatggaagc gattggagca agtgcattat ctattattac
    20821 accatacttt gttacaccaa cacaacagga attaatctta cattacaaaa ctattgctaa
    20881 ttcagttaat ttacctatta taatgtataa tatgccaggt aagactggaa ttaatattga
    20941 accagagtca gtacgtgaat tatcaaagat aaagaatatt gttggaatta aggatagtag
    21001 tggtaaactt gacaacatga aagcttatat tgaagcaacg aagaatgaga attttagtgt
    21061 gttttcaggc tcagattcac ttattctgga tagtctaaaa gctggtagtc aaggagctgt
    21121 tgcagcaaca acaaacttcc tcacagagat tggtatctct atctatgaaa actttatgaa
    21181 aggggatttg gaagaagctc aaaaagctca aagtagtata gaagaacttc gacgtatact
    21241 gaaacttgga acaattccat ctgttatgaa gaaaactatt gtcttgaatg gaattaatgt
    21301 tggaactgca cgacttcctg taactgaacc aactggggaa gtacttgaag aaattaaacg
    21361 agtagttgaa aattatagga caatacttaa taaaagtagt gagaatagat aatataaata
    21421 gggaaattac actggtatat aattaatata tatattataa aacatgacta aaattaatga
    21481 ataagtaaag tggtttgatg agtatagagg tgataatatg aaaaagatga atataaccaa
    21541 aagaatgtca gaatgtgggg ctcttgcaat tgtaagagaa gaaaatctta accgtgcatg
    21601 tgaaattgct gaaggatgta taaaaggtgg tataacagtt atagaaatga gttataccct
    21661 aaataatgct ggagaaataa ttcaagggct taataaaaag tatggtgaaa cactttgtgt
    21721 gggagcagga acagttttgg atagtgaaac agctcgtcat gctatattac atggagctca
    21781 atttattatt gcaccaaatt acaatgaagg tgtagcgaag atttgcaata gatatcagat
    21841 tccttatgct ccaggatgta catcattgac tgaggcagta gacgcactaa gtttaggagc
    21901 aacttttata aaagctttcc caatatcaga tttctatgga cctaaactag taaaggtatt
    21961 taagacacca atacctgata tgccagtttt agcaagtgga gggcttaata ttgaaaacct
    22021 acatatatgg ttagaaaatg gagttgatgt atgtggattt ggtggtttat taactaaagg
    22081 ttctgttgaa gatattgcta aaaatgctaa caagattcgt gaaatcatta aaaatgtaag
    22141 aaaaatttaa catagggctg tatcatagta tcttataatt actatgatac agccttttta
    22201 atattattca aatttaaaac agtttacatt tctattatat tacatataat ttcagtaatt
    22261 ttatcttaat ttttctatct ggaaaaaaat gatttaaaac ttaagttatg atataatttc
    22321 attaaaagaa ggcgattttt agtaaatatg agaaaataat gtgtttttag gggaaaagtt
    22381 aaatacagat ttaagtgaac aagaatcgat aattaagata ttaaatggtg aatagggcat
    22441 caaaaaaatt actaaattaa aagttttaga gggagaatct tagcaagtgt gtgtgaaaat
    22501 tcaatttcaa acaaaaaata attgtaaatg aaatcaattt atacacgttg aagattcact
    22561 tttatcatta tattaaaaag cagagtgaat taaaagtgag tctaaagata ggaggataat
    22621 taaaacaatg aaagaagaca ttaggttaca tcgaatgtgt aaaaaagaac aagcaacaaa
    22681 gaatgaagtt atatcagagg atattctaaa atctatgagt attggggtca gtaaacattt
    22741 tttagatgat tcatttacaa taatatgggg aaatacatca ttctatcaat tattaggata
    22801 tacagaagaa gaatttttag ttaaattttc tagtttaaaa gattattatg aacagaatct
    22861 ttatcatttt gaatctatga aaaatcattt tttagaggct tatcatcatg gcgaaaagag
    22921 tatagaatat caaatttgca tttcagcaaa taatggagac cctgtttggg taataatgac
    22981 tggtatatta attgaatcac aagataatga gaaatcaatg gtctatatat tgtattcaaa
    23041 aattaatgag ttaaaaataa agcaagaaga actatctgat atggaaaaag agagtacaga
    23101 gaattttaaa tggatgatgt cagagtatgc tgggaatgtt tatattagcg atatggatac
    23161 atatgaatta ctatacttaa ataagcactc atgcaatact ttacaggcat cagcaaatga
    23221 acttattgga agaaaatgtt atgaggtaat tcaaggtaga acttcgccat gtccgttttg
    23281 tacaaattcg tatttaaaaa aagatgacat atatgaatgg gaattttata ataaaaaact
    23341 gaaacgaaca tttataatta aagaccgtat gttgaattgg caagggcgaa gagttcgtat
    23401 tgaattatct tatgatatgt atagtgctga atataaatta gcaaaaaaag atcaggaaag
    23461 agaatcaatt ttgaaaacta ttccagcagg aatggttaga ttagatgcaa gagattatct
    23521 tactatactt tggtgtaatg atatattttt gaatatgatt gaatatacaa aggaacagtt
    23581 tgcagaggaa cttaataatc aatgtggata tttattttct gaagattata aacgtagtaa
    23641 gaaactggca caagatttga aggagtctgg ggataatgtt gtatttgaag caaaaatata
    23701 tacacgttca agggaagaac gtatatggac tgttacattg tgttatatca gtggggaaga
    23761 cagttgggat ggaataccat ctttttatag tataggtatt gacataacaa aggaaagaaa
    23821 acagattgag aaattacagc atatatcaga gaaagatgca ttaacaggaa tttataatcg
    23881 tgctgagact gaaaggcaga ttaagaaata ttttgaaaaa aatttaaatg ttatgggtgc
    23941 actgtttatg attgatacag ataattttaa acagattaat gatacagaag ggcatatgat
    24001 tggagatata gtacttactg agatggcatc aggtatgaaa aaaataatgc gtgacagtga
    24061 tgttgttgga agaataggtg gagatgagtt tacaattttt atgaagaata tttcctcagt
    24121 taaagatgca gaaaagaaag cagaagaact attgtatatg ttccgtcatc tatttcagaa
    24181 agaaaaaagt tttctaaaag taacttgtag cataggaata gctatatatc caaaggatgg
    24241 tactacattc aaggaaatat atgcaagagc tgataaagcc ctgtatcaag caaaaaatat
    24301 gggaaaaaat aattatgtaa tctacaacca agattatttt aaagaattag aagaatttga
    24361 ttgttcttct cttggtacag ttattgattc agaaaaacaa tacgctgaat atcctgataa
    24421 tcttacacgt tatatattcc gtatgcttta tcagactgat gatatagacc aggcgattaa
    24481 tatgatactt gaggttgttg ggaaacagtt tgatgtaagt cgtgtttata tttttgaaaa
    24541 tacagaagat ggtagatata cttctaatac ttatgaatgg tgtaatgaag gaatctcttc
    24601 tcaaatggct tatttgcaaa acagcaatta tcaagactat gaggattatg aaaagctttt
    24661 tgaggatgaa ttagtttttt attgtcgaga tatccataca ttaagtccta aactagaaga
    24721 attgttttct agtcaaggta tccactctac acttcaatgt gcatataaag aagatagagt
    24781 tttcagtgga tttgttggat ttgatgaatg cacaggacta cgtctatggt cacaagagga
    24841 aataagtaca ttatcattga tttctcaaat tatatcaatg tttttacaac gtaaaaaaaa
    24901 taataaactt aatcaacaga ttcaacaata ccagaatatt ttagataata ttgaccagtg
    24961 tatttgtgtg attaataaag aaaataattt tttattatat gtaaataaaa aatttaaaaa
    25021 tacatattct aaattccaaa ttggacaacc ttattatctt gattctatag atattcataa
    25081 gatatccatt atatggaata aaaaagaagc atacctatgt actagcacga tttaatggta
    25141 aagtaaagtt tatattggac ttaaattaaa tttcgtttta aataagatat tattttagtt
    25201 gcttactgaa aagactagaa tctatattaa tgaattatac taattttata ccactattaa
    25261 aatattgttt gctaaaaata aaaaatagcc ttaaaacagt tgattataaa aaattaactg
    25321 ttttaaggct atttttatct ttgtgacgaa atagacactt gccatttaga atgtgacaat
    25381 atagtagttg tttagaaaag gcacaaagca ttatacttta actaaagtaa aatgtatttt
    25441 gcttttagaa taaaaacaat taggagggag ctataattga aaaagataat atatgtatta
    25501 tttacaattg aactattaat agtatcttta ttaggtctaa atatagttaa agataatgaa
    25561 attaataata ttttatataa cgatacaaca agtatatctg taatgtttaa agattataaa
    25621 aagttaaata aaaattatag taagtggatt aaagatattg cagatgaaaa taatgtggct
    25681 atatcaaagt atgtttttaa taataatgaa aaattatcta tatatacgac tgatacaagt
    25741 ttgaataaca atataaagtt aaaatcagga aaatttccta atactaattc aaatgaattt
    25801 attagtaata taaacactaa taatagtaat caagtaggta aattctcaac tatgagtaaa
    25861 gacatatcaa ttttaattag ggattttggt aaaataagtg aagttggaga atcaggaatc
    25921 ttatatattt caactcaaaa tgaagataca ataaataaaa tattaagaga actaaatgta
    25981 gacagaagta tttttgtagc aagattacat gatagatatg ttaactcaaa tttatactta
    26041 aatccttcaa taattagaga tttaatacta attatccttt gtttcttggc tacaataata
    26101 cattattcaa ttagtgtgag tagagaggct tctattttaa gattaaatgg atactctaag
    26161 ctagatataa ttgctagagt tataaaaaat atacttagaa ttatgatttt atcatctgtc
    26221 acagcattat taatatatat aatctatatt ttaaaattaa atataggtgt tagagcatgc
    26281 ttatactttg ctatgttttc gattatatgt attttgataa atattttgat atctatatta
    26341 atagttggtt ttaatagtag aagttctaaa tatgtgctat ctctaaatgg aaaaaaatct
    26401 tatggttttg taaatataat gcatttcaca ttgaaaattg tatttgtatc atttcttata
    26461 ttatccataa ataactgtat tttaaattat aatatgctac aaactcagtt aggaaatcta
    26521 tctgagtggg ataaagcaaa aaatgtttat aatcttactc ttaaagatac aggcgaacaa
    26581 tctttaggaa aggaagaagt aattaaaaat gccaagatta aaggtttcta taaaaatttg
    26641 gtagaagaaa aagatgcttt cttaatagat tcaactaatt ttgaaaaact tgaagatgga
    26701 agttacatgt atgaaataaa ttcaaagggt aaaaacccag aagtaagtca atatggtaaa
    26761 aatataaaaa taaataaaaa ctatcttaaa gttaacccta tttactcaaa tggcaaagaa
    26821 atatttaatc taatcgatta tgacgataag actataaatt tattagttcc taaaaagtta
    26881 caagtacatg aaaatgagat taaaaaagag tttagagaat tattttattt tgaaaaaata
    26941 gaagtagaaa atatgtacaa taaagagtta aatagagagt taaataaaac aaagaagtca
    27001 gatttaaatg ttaatataat atatgtagat aataatcaaa gctattttac ttataactcc
    27061 cttgttatgg atgacaatag gaatctcatt gatgacccaa tagcaatagt tgacatagat
    27121 aatattgatg attcttttta tctttcttat atatctagat gtgtatattt taattctaag
    27181 aaattagatg cccttgcaga tataagtaac attattgaag ctcaaggtgt agaagcgcat
    27241 atccaaagtt tacatgctgt ttataatgag tatggtttag aaataaataa actagaaaaa
    27301 tatttgaata gtgaaatttt tactataata ataatagcaa tatcaaactt aatgattacc
    27361 tacaatatag tagctagcta ctatgaaaga aataaatata agttatatat taaaaaaata
    27421 tttggttact caactacagc tagaagtatg ttgttaatag tctcattaat attaacaaac
    27481 attattccaa taggcattat atcaacaaga gtagatttaa gtaataatat tatcttattt
    27541 ggacttctaa tattagttat agaagtaatt gtatctattg tattagacaa gattatctca
    27601 aatagttcat ttaataaaat tataaaagga gaacattaaa aagatgattt cattaataaa
    27661 tatatctaaa aaatttaaag ataaatctat aatagacaaa tttaacctag aaatttctaa
    27721 gggcgagttt gtagcgataa caggaaatag tggttcaggt aagacaactt tacttaatat
    27781 aatgggatta ttagaaaaac ctaattctgg tgatattata ataaataata taaaaaatcc
    27841 aaatagtaaa cagataagaa ttttacaaag ggatttttat ggctatctat ttcaaaatta
    27901 tgctttaatt gagaatgaaa atgttgaaaa taacttgaaa attgctttaa agtatcaaaa
    27961 aaatatcaac cataatgaaa aaataaatta cgcacttgag tctgtaggtc ttagaaaata
    28021 caataaaaaa aagatatacg aattgagtgg aggagaacaa caaagggtag cattagcaag
    28081 agtaatatta aaagaatgtg aagtaatatt tgcagatgaa cctacaggta atttagataa
    28141 aaaaaataga gacatagtat ttgatatact taaaagccta aataagaatg gtaaaactat
    28201 cgtatttgta actcatgatt tagagttatc ggagcaggca gatagagtta taaaattgta
    28261 ataatatcaa ctataataaa tataagaaag agattacagc taaatcaagt ttaataataa
    28321 tacgaataga atttaaacat taagtaaatt agtagtttgt aaatataagt ataatatgat
    28381 atcataaagg ctcaaaagaa attttatata ataagaaatc taaaaataac aaatggagga
    28441 catataaaga tggaaaattt accaaattgc ccgaaatgta attcagaata tacttatgaa
    28501 gatggaactc ttttagtttg tccagaatgc tctcatgagt gggcattagg attgggagct
    28561 gaagaagatt caaatattat caaagatgta aatggaaatg tattaaatga tggagattct
    28621 gttacagtaa ttagagacct taaagtaaaa ggaagttcat catctataaa gattggaaca
    28681 aaagtgaaaa atatccgttt aatccatgac tcatcagatg gacatgatat agaatgtaag
    28741 atagatggtt ttggagctat gaagctaaag tcttctgttg ttaaaaaagc gtaatattat
    28801 aaataagctg tagtgtttat tgtttttcag tacagtataa aaccataaga atctattaag
    28861 taataaaagt ttgtttattt atctttatat tttcttatgg ttttattaaa tataaatttt
    28921 attgaacacc aaaatattct ctcctctaca aataagccaa tcttatttaa actaatatac
    28981 tttgtactat ttatactgaa attgtaaatt taaatgctat gttatttagg tttgtatagt
    29041 taatttatat atttctgatt ttataaattg tatttattat tatatcttga ttctaaaatc
    29101 attaaaaatt tcaacttcta taaagattta caatttatca aatacattat gagtatataa
    29161 acaatttatt attacagtgc tacatttaaa ataagtctta gtataaaggt attgttacaa
    29221 gtataaagaa acttatgtat gctattggaa taaataatac cttaaattat aattaagaat
    29281 aagaactgtt aaataaggga cttgattatc atttagttat agataatata atattaaaaa
    29341 taaagtttaa gaggagaaaa aatatgatag atatacataa aaatatatat gataataagt
    29401 tatttgagga gctaaagatt gactgtaaga agtgctttgg actttgctgt gtagctcttt
    29461 atttttctgc ttcagatgga tttcctatcg ataaggaatc aggaaaaccc tgtattaatt
    29521 tacaaccaga ttttaaatgt tctgtacata atagtcttat gaagcgaggt tttaaagggt
    29581 gtactgcata tgattgtttt ggttcaggtc aaaaagtagc acaagtaaca tataagggga
    29641 tagactggat gcaatcacca gaattaacaa atcaaatgtc tgaagtgttt ttaattatga
    29701 gacaattaca tgaaatgtta tggtatctta aagaagcatc tgtattaaat atatcagata
    29761 caatcaaatc aaaaattgat ttaataattg aagaaactga aaagataact aatatgggcc
    29821 cagaacagat aattaatttg gatatcatat cacatagaac aaaggttaat ttattattat
    29881 cacaagcgag tgagtctgtg atgggaaagg ttaaatcatt tatcaagact tctactctta
    29941 aaaacatgaa aaaattaagt aaaaatatag atttaatagg agcagacctt agaaaaataa
    30001 atctaatagg agcagattta agaggtagat ttctaatagc agcaaattta agaaatacag
    30061 atttgagtgg ggcaaattta ataggagcag acttaagaga ttgtgatatt agaggtgcta
    30121 atcttgaaaa tagtatattt ttaacacaat tacaagtaaa tactgcaaaa ggagattcta
    30181 gtacgaagtt accagcttca ctaatacgcc ctaaatactg gaaaaaataa ttaaattaat
    30241 acataatttt acaaaatatg aacttttttg actttaattc atattataca ttagagattc
    30301 tttacaagaa tttcttaata taatataggg agagatataa aaatgagtta tcaaaattat
    30361 aaatgtgaaa gagattatgt gtatgatgat tataaataca ataagtgcaa caagtgctat
    30421 aatgattatg aggaaatgac acatgttcat gaatattcag aaagtgttaa gttagcagaa
    30481 gaatgtgagg atagacataa ccaccgtgca gcaggggtta ctggtgaagc tataccaata
    30541 aatggaggta caaatcatgt tcataaaata aatgataatg tagatttcct tgaccatttc
    30601 cataagatat gtgttacaac agggccagca attagaatac ctggaactga taaacacatt
    30661 cacttaatat gtggagaaac tactgttaat gatggtcatt gtcataaatt cctttttact
    30721 actcaaatag aagcaccttt agtataagct gatttgaatt attgtagtga atttgctaga
    30781 ttatagtata ataatatata atagataata tttaaactta atgaggacta tgatatttaa
    30841 ccttcgtagt cctttctaag ttttgtataa aaaagtgtta tgcaattaaa agtgatatag
    30901 ggaggaaatt tttatgaaat ctggaataat tcttgaaatg aaacaaataa attcaacaga
    30961 atatgacgaa atgttgcaag tctgggaatc atcagtaaga gcaacgcatg attttttaac
    31021 agaaaaagat attgaatctt tgataccatt agttgagatt ggacttaaag aagtagaaaa
    31081 tatagtttgt ataaaagata atgagataat aaagggcttt ataggaatag ataaagataa
    31141 aattgagatg ctctttatag aagataaata tagaggaaat ggtattggta aaaaattaat
    31201 aaaatatgct atagataaat acaatgtaaa atatgttgat gtaaatgagc agaataaaaa
    31261 agctgtagga ttttatattc acttaggatt taaagtgttt gataggtctg aaatagatgg
    31321 acaaggtaac ccatttccaa tactacatat gaaattaatt taaagttata cttctcactt
    31381 attcgcaaaa atatttactt aaatttttat acaaataaaa gtcgtgacag tcttaggaca
    31441 cgactttttt actacactat taaattgaaa tttatctgtt ctatattatg attattagat
    31501 gtagtttttt tatttttact atgtacttaa ataccaagta tacccatacc tatcaagtac
    31561 ttcagcagcg ccatcatttt gatgtggtgg tgggtgtggt aaaaattctt gtacaagtgt
    31621 accaccttcg gcaagtttta cagatatttc ttctatcgct tctcgagatg gtagaacaac
    31681 acaaatcgcc atgccagaaa ttacaacagg cttgtctgta tccgcaccca ttatacaacc
    31741 ttcgtatttt gtgaaagtaa cacttgcgtg cataacagga ttgtttggat tgctgtctaa
    31801 tcgcgcaaat atagtttttc ctcctccgaa agcctcagca taaaaattaa atgctttttc
    31861 acaatcacca ttaaatatta aatatggagt tagcatgacc aatatatcct ccttgatata
    31921 gaataattta tagttgcacc tgataatcta aattatgatt tagtaaattg tatcataggg
    31981 aaaccttgaa gtatataatt ttttattatt atattataca aatttattaa tttaaaaaaa
    32041 tgttatatgt acggaaggtt tacaggataa aatataataa aaagattaag gtgacattat
    32101 aaaaaatgta tattgacatt gacgctacgt aattgtatat actttgtctt aaaggagatg
    32161 gtaaatatga atattataga aattcgctca gataaaatat acaagaagat aatggatgcg
    32221 ccaataaaca aaaaagaaga tatatacaga tatgaattaa tgaagccttt tgaatttaag
    32281 tggaagtgta tgaatgttcc aatagttgct agacagaaag gtggatatga tgtaattata
    32341 gcaagtgaaa tgttaggggt tttatcgcct aaggatattg atgaaaagca aaaaaagaat
    32401 ataaatgtgt tatctgctga taaaatttgg ggcacttgca aagaaaccat agaaaactct
    32461 ataaatgctt ttataaaaga agggtatgat ttaaacatta aggactataa atattcaata
    32521 ttattggcga atccaaatag tccttataca atattaagtg atggatactg gggtgatggt
    32581 gggattcctg gatatatatt tctatcattg gttcctaatg aatatactat caatagatta
    32641 ccagtattaa tagcacatga atgtaatcac aatattagat ttcagtttat agagtggaat
    32701 aataatataa cattagaaga aatgatgata aatgaaggtc ttgcagaaaa ttttgcaaca
    32761 tggatgtttg gagaggaaat gttaggacct tgggtcagta gaacagatat cgaaacatta
    32821 aatacttata taaagccaat aataaaaagt gctttaaaag aaactggatt tcaaaatata
    32881 acatcttatc tttatggtga tgatatagct aaaatgcaag gatattttcc agtagggttg
    32941 ccttattgtg caggatatgc ttgtggatat tatatgatta agtattattt agaaaagaca
    33001 aataaatcaa taatcgaagc gactttattg ccttatagtg agataatcga agcagtaaaa
    33061 gagttttggg aataaatttt ataaattgca taatttgaaa taaattatgc aattatttta
    33121 tttttaagaa aaaataaatt gacaataa
//
LOCUS       DACXMZ010000014        33086 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.014, whole genome shotgun sequence.
ACCESSION   DACXMZ010000014 DACXMZ010000000
VERSION     DACXMZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.014"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            183..1004
                     /locus_tag="JD016_02670"
     CDS             183..1004
                     /locus_tag="JD016_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAU4998534.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    1083..1294
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1381..2697
                     /gene="argH"
                     /locus_tag="JD016_02675"
     CDS             1381..2697
                     /gene="argH"
                     /locus_tag="JD016_02675"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAU4998535.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            2946..3284
                     /locus_tag="JD016_02680"
     CDS             2946..3284
                     /locus_tag="JD016_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HAU4998536.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(3411..4700)
                     /locus_tag="JD016_02685"
     CDS             complement(3411..4700)
                     /locus_tag="JD016_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAU4998537.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            4780..5484
                     /locus_tag="JD016_02690"
     CDS             4780..5484
                     /locus_tag="JD016_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HAU4998538.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKLNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            5576..6622
                     /gene="selD"
                     /locus_tag="JD016_02695"
     CDS             5576..6622
                     /gene="selD"
                     /locus_tag="JD016_02695"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5624..5626,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAU4998539.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            6649..8052
                     /locus_tag="JD016_02700"
     CDS             6649..8052
                     /locus_tag="JD016_02700"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAU4998540.1"
                     /translation="MSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREVIDL
                     KRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLGRSL
                     LSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTNTIKRLTGAEDVLVVNNNAAAV
                     LLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYKEAI
                     TEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKYGLS
                     YEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDKLTI
                     CALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINIEDG
                     FSQVGGGSMPLETISTKVISITPEYMNVSSLEKKLRLSEAHIIARVYDNKYVLDVRTI
                     FDDEFDVIVEELRKAFN"
     gene            8166..10049
                     /gene="selB"
                     /locus_tag="JD016_02705"
     CDS             8166..10049
                     /gene="selB"
                     /locus_tag="JD016_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAU4998541.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            10154..12892
                     /locus_tag="JD016_02710"
     CDS             10154..12892
                     /locus_tag="JD016_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAU4998542.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLVSLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(13011..13955)
                     /gene="manA"
                     /locus_tag="JD016_02715"
     CDS             complement(13011..13955)
                     /gene="manA"
                     /locus_tag="JD016_02715"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAU4998543.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIIYSHI"
     gene            14119..15489
                     /locus_tag="JD016_02720"
     CDS             14119..15489
                     /locus_tag="JD016_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAU4998544.1"
                     /translation="MIIMELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFG
                     KGVYDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSE
                     PFKLNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKC
                     IKHYYSKELYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDK
                     VYIKNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSE
                     NIDEKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYP
                     EILTLEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDL
                     KNDVRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAII
                     TLCNKI"
     gene            15577..17466
                     /locus_tag="JD016_02725"
     CDS             15577..17466
                     /locus_tag="JD016_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAU4998545.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAIKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEEGSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            17481..17936
                     /locus_tag="JD016_02730"
     CDS             17481..17936
                     /locus_tag="JD016_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAU4998546.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            17995..18312
                     /locus_tag="JD016_02735"
     CDS             17995..18312
                     /locus_tag="JD016_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAU4998547.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            18337..19599
                     /locus_tag="JD016_02740"
     CDS             18337..19599
                     /locus_tag="JD016_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAU4998548.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GATFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            19704..20768
                     /locus_tag="JD016_02745"
     CDS             19704..20768
                     /locus_tag="JD016_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAU4998549.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            20822..22000
                     /locus_tag="JD016_02750"
     CDS             20822..22000
                     /locus_tag="JD016_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative C-S lyase"
                     /protein_id="HAU4998550.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(22191..23249)
                     /locus_tag="JD016_02755"
     CDS             complement(22191..23249)
                     /locus_tag="JD016_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAU4998551.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENDKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            23316..23687
                     /locus_tag="JD016_02760"
     CDS             23316..23687
                     /locus_tag="JD016_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAU4998552.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            23724..24398
                     /locus_tag="JD016_02765"
     CDS             23724..24398
                     /locus_tag="JD016_02765"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAU4998553.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            24647..25486
                     /locus_tag="JD016_02770"
     CDS             24647..25486
                     /locus_tag="JD016_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF348 domain-containing protein"
                     /protein_id="HAU4998554.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            25730..25826
                     /locus_tag="JD016_02775"
     tRNA            25730..25826
                     /locus_tag="JD016_02775"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25764..25766,aa:Sec,seq:tca)
     gene            25977..26684
                     /locus_tag="JD016_02780"
     CDS             25977..26684
                     /locus_tag="JD016_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HAU4998555.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(26826..27662)
                     /locus_tag="JD016_02785"
     CDS             complement(26826..27662)
                     /locus_tag="JD016_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAU4998556.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            27850..28755
                     /locus_tag="JD016_02790"
     CDS             27850..28755
                     /locus_tag="JD016_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HAU4998557.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            28764..29948
                     /locus_tag="JD016_02795"
     CDS             28764..29948
                     /locus_tag="JD016_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU4998558.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            30069..31364
                     /locus_tag="JD016_02800"
     CDS             30069..31364
                     /locus_tag="JD016_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HAU4998559.1"
                     /translation="MIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQGV
                     VKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGRYI
                     KSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEVEN
                     LTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYCIM
                     VGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLSFL
                     ATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPFVG
                     VIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIENPKFYI
                     VVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            31318..32361
                     /gene="holA"
                     /locus_tag="JD016_02805"
     CDS             31318..32361
                     /gene="holA"
                     /locus_tag="JD016_02805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAU4998560.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(32548..32814)
                     /locus_tag="JD016_02810"
     CDS             complement(32548..32814)
                     /locus_tag="JD016_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAU4998561.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT        13226 a         3365 c         5604 g        10891 t
ORIGIN      
        1 atattcagaa aaatactaca agaaacgatt tattttgctc ttaaaataag ttaaatgaat
       61 gttattaatt ttgtaaattg aaatagttta agtataaagt tatgatataa tctaataagg
      121 gtaattcttt agattatatt aaggaaatat aggtatacaa taaaaagtta tggagttgga
      181 ttatgggata taaattaatt gtaacagata tggatggaac tttattaggc aataatcata
      241 aagttacaga tgaaaataaa actgcactac aaaaagttat aaaaagcggt attaatgtaa
      301 ctttagcaac aggaagagcc ttcgattcag ctaaatgcaa tgtagatttt cttaaagagg
      361 acatgccaat aatagcttgt aatggttcat taataagaga gcaaaatgga aatataatat
      421 attcaaataa gattgataca agaacatgtt taaacatttt agatgtatta gataaatacg
      481 atatttacta ccaatgtaat agtatagatt ctatgctttc gaaaaaaata gaaggacgtg
      541 aagatagatt aagtgtattt ttaggctctg aaacagaagt aattgtaaaa gatgatttga
      601 gagaagaaat ctttaaaaaa gatatactaa aatttgtaat catagaagaa aaaaatccat
      661 ctattttgga tgaaataaga aaagaattga gaaaagttca aggaataaag ataacttctt
      721 catggccaaa caatattgaa gtcatgaatg aaggtgttga taaaggtaat gcagttaaaa
      781 ttttagctga aaaaatgaat atagacagag aagatataat tgcatttgga gacaattaca
      841 atgacataga aatgataaag tttgctggtc ttggggtagc tatgggaaat gcagaagaat
      901 tgattaaaca agaagctgat tatgttacag atacaaatca agacagtggg gttgcaaagg
      961 caatttataa attcctagag ttaaaagaat cacttagctc ttaaaacagt atataatagg
     1021 aaaaaatagg ttgactattt tgtagatagt aattataata caaatagata aaacataaaa
     1081 atttagattc agtgaaaaag agagtagata tagagaaaat ctcagcgaat tgggggatgg
     1141 tgtgagccta atgataacac tttatattga aaagaacttt ggagaatact acttgaaatt
     1201 cagtatggta gtaggtaatc tcgccttaag agaatagagt gggtaaacat tgtttaccaa
     1261 ctagagtggt aacacggaat atttcgtctc ttggtctttt tgaccaagag attttttagt
     1321 ttcattgagt tacaagactt ataattaaaa tacaaaaaat taaaatatga ggtgaaagtt
     1381 atgaaattat ggggcggacg ctttagaaaa gcggaaaatc aacttatgga agaatttaat
     1441 aaatcttttg gctatgattg tgtactgtac aaaaaagata tcgaaggaag tgtagcacat
     1501 gttcatatgc aagtaaaatg tggactttta acagaagaag aaggaaagtc tataacagaa
     1561 ggtttaaaag ggatacttga agatgtagaa aatggaaagt tagtattgga tggagagtat
     1621 gaagatatac atagttttac tgagataaat ttaattcaaa gaattggaga tgttggcaag
     1681 aagcttcata cagcacgtag tagaaatgac caagttgcag ttgatatgag attgtatgct
     1741 aaagaaaaag caaatgattt ggtggattta atatctgaat ttaaagctac tataaaagat
     1801 gttgctgaca aaaacccagt gatgatgcca ggctatactc atcttcaaag ggcacaagta
     1861 gtgacattta agcatcactt aatggcatat tatagtatgt ttgatagaga tgagaaaaga
     1921 ataaaaaatg ctatagaaat attagatgaa tcacctttag gctgtggagc tttagcagga
     1981 actacgcatg atatagatag aagtataact tgtgaacaat taggatttaa aaaagtagta
     2041 gataatttta tggatggagt aagtgataga gattatttac tagagctaat gagtgatttt
     2101 tcaattataa tgatgcactt aagtagatta agtgaagagt tgatactttg gagttctcaa
     2161 gaatttggat ttgttgagat agatgattta tatacaactg gaagtagtat aatgcctcag
     2221 aagaaaaatc cagatggtgc agagcttata agaggtaaga ctggtagagt atatggaaac
     2281 ttatttggtc tttttacagt aatgaaagga ataccattag catataataa agacatgcaa
     2341 gaagataaag aaggtttttt cgatagtgta catactcttg aaatgtgtat acaaataatg
     2401 gatagaatga tagccacact aaaggtaaat gaggataaaa tgaaacaagc agtaaaaaat
     2461 ggttttttaa atgcaacaga agttgcggat tatttggtta aaaataatgt tgcttttaga
     2521 gatgcacatg gtatagtagg aagtatcgtt atatattgtg aagataataa aaaagcaata
     2581 gaagatttaa cattagaaga attacataag ttttcagatg cttttaagga agatatttat
     2641 gattttatcg actatgagag tatattaaat aagggaataa aaaagaattt aaaataatgt
     2701 atttattttt aaaagctgta aatttgaatg ttatcattta aatttacagc tttttattgt
     2761 gtaagatttt attatataaa agtaagaaac ttaaaaggag tagtatttaa aataaataca
     2821 aataaaaaat tattattaaa atgataatta atatcaataa gtattgacaa aattatttct
     2881 aaatactatt atatataata taaataataa tgaaaatcaa tatcaaataa atgggaggaa
     2941 atctaatgaa cttattagtg attggtggtc atgaaagaat ggaaaaagat tatatggtta
     3001 tggccaagaa aaaaggttat aagacaaagg tatatactac catgtcatct aaattaaata
     3061 attctatagg aagaccagat gcagttgtca tacttacatc aactgtttca cataagatgt
     3121 ctagaacggt tgagtctcaa gctaaaaaac aagatatatt aatcgtaagg cataaaaata
     3181 gtagtaaggt agcatttaat gaatgtctag atatggttga tgaatgtctg ggaaattgtt
     3241 caaaatgtaa gcttaaagac aaacaaaaac aaaaaaatat ataggtataa aaaatacata
     3301 aacataaaat taaaagtact gagataatag ttataattat tatctcagta cttttagatt
     3361 tataaggata agtatagtat aaatttttta ttataatttc caactacttg ttactttttc
     3421 atattttttt tacttctagc tttttttctt tttttcttct ttatatttga agctattata
     3481 aataaaacta aaagcactag aatacctgca ataatcttaa caaaagtcat taaaacatta
     3541 ttttcagtca aaaatggaag actgctatca agattatttt ttgcaattaa gtctatactg
     3601 ttttctagtt taccattatt atatacattt aaagtaccaa ccttagctcc tttttttata
     3661 ggcaaatcaa ttttatttaa ctctgttttg gtatcataat tgccttgaga ttcatctttt
     3721 tctaagaata tcctataact attttttggt tcataaatca attcatgttg ttttgtaaac
     3781 aatacttttt tagtatctgt gtatttttct ttatcaacaa tagtagcaca cttaaagtta
     3841 tcaaatccat aatcaatcag aatacgtgag tctaaatata aatcagcatt agttgaatta
     3901 aatacagcaa cgataactct tctgccatct tttacagcac tcgataaaag acatttacca
     3961 gcagcatcag tatatccagt ttttatacca tcaactatat catattttat atttataggt
     4021 tgacctttgt atgtaatctt atcatgtgat gtcaaaaact tattagtgtt tacaaagtat
     4081 ctctcataag gatatgcttt tgtagcttca aatttaagtg acttagtttt tacaatttgt
     4141 ctaaaagtat cattattcat agcttctcta gctatgagag ccatatcatg agctgttgtg
     4201 tagtgatttg ggtctggtaa tccatgagga ttattaaaat gagtattagt agctccaatt
     4261 ttttttgcct cagagttcat aaggtcaata aatttttgta cattgccacc accaacatat
     4321 ctagctaata caaaagcagc atcattagca gaatgaacca aaagagcatc tagtaactgc
     4381 tttacagtaa aagattcccc attttttaga tacatactag agccatctat ttgaggtaaa
     4441 tctttaattt ctattacttg atttaaatct ttaacctcct ttagaactaa acaagctgtc
     4501 catactttag ttgttgaagc tggataaagc ttagaatttc catccttatt atatagtatt
     4561 ttgccagact catagtccat taaaactgca tgtttagcaa ctatagctgg ttcattatct
     4621 gcaaaagaga tattagagct tcctaaaaat gaagtaaata tcaataaaca gattaataaa
     4681 agtgataaat ttcttttcat tgtgagcctc caaattttaa attcatcttt tagtatagca
     4741 tatatttgtc aataattaaa agaaaggagt gattaatata tgaaaaaaaa ggtaaaattt
     4801 atttcgtttt ttgttttatt tattttaata atatcatcaa taatcgttta taaatcgctt
     4861 attttcaaag atgatattta tgttgtaagt aaaaaaaatt tacctaatga aaaaacacat
     4921 gttgtagaga atgaaattaa aggtgaagat aataatcaca gtaaagtaac taaagacata
     4981 gataaaaatt ttcaaaataa taaaataact atatacataa gtggagcagt aaatagacct
     5041 ggaattgtaa ctatagagtc agacaagaga ctttatgatg ctgtagaatt actaggagga
     5101 acgacaaaag aagcagattt aaatggagtc aatctatcag taagattaga agatgaacaa
     5161 cattacataa tacctaaaat aggtgaagca acatctgtta caagtaatga tgattccaat
     5221 aaactaaatc agaagaatga gtcaagaggt gagcctaaaa ataaatccaa taaaaatata
     5281 tctaacgaat caaaagtaaa tataaatgta gcgacgatag aagagttgga ttctctacct
     5341 ggggttggag aagctacagc caataaaatt ttacaacaca gagaagaaaa tggacagttc
     5401 agttcaattg aggaaataaa aaatgtaaat ggtattggag ataaaaagta tgaaaatata
     5461 aaagatttaa tttgtgtaga ctaaaatata atacaaaact tacatataga tatgttatta
     5521 taaatatgta tgtatatgtg tgtatatgtg gaaatttagt aaaggaggca cttttatgaa
     5581 aaaagaacaa aaaaaactta cagagatgac aacagcaggt ggttgagccg ctaaaatagg
     5641 gccagaggtt ctggcatcag ttttatctca attgcctaaa aatgataata tagaaaattt
     5701 attagtagga ctagatacag cagatgatgc tgcagtttat aaattaaatg atgatatggc
     5761 acttatccaa actttagact tctttacacc aatggtagat gacccatacg tatttggtca
     5821 aattgctgca tcaaattcac tatcagatgt atatgctatg ggaggcaaac ctttggttgc
     5881 tatgaatatt gtttgttttc catcatgcca tgatatggat gtacttgcag aaatattaaa
     5941 aggtgggttt gataaagtga aagaaagtgg agcacttttg gttggagggc atactgtaga
     6001 tgacaaagag cctaaatatg gtctttcagt ttctgggatt gtaagtccaa ataaagtttt
     6061 atcaaatgct actgcaaaac ctggtgataa gttaattata acaaaaccaa ttggagtggg
     6121 tgtattaaat acagccatga aagaaggtat ggtagaacaa catatagcag ataaagtaat
     6181 agaaataatg atacatttaa ataaatatgc agcaatgagt tttgagaaat ttgatgtaaa
     6241 ttcggttaca gatataactg gttttggtct acttggtcat actttagaaa tggccaaagc
     6301 ttctgaagta agtattgaaa ttgaatcaaa acatgtacca attattgaag gtgcaataga
     6361 aatggcacaa atgggaatta tacctgctgg gatgtataaa aatatgcatt atgtatctaa
     6421 agatgtagaa gttgtaggaa atatagaagt tgcagttcaa gatattttat acgacccaca
     6481 aacatcagga gggttgttaa tttcagtaaa agaagaactt gcagaggaac ttgtaaaaga
     6541 tatgaaatta aatggagcaa tagaagctaa aattataggt tctgtagttc caaaagggga
     6601 aaaatatatt acagttttat aaaatattat agagaaagag tgattgtttt gagcaaaaga
     6661 gaattgtttg caatgttgcc ttcagttgat gaagtattaa gtgataatag aattgtagaa
     6721 ataataaatg aatatcctag aagtttagtt ttagaatctg tgagagaagt aatagattta
     6781 aaaagacaat ttatattaag attaaaagaa gatgcatcaa actctgtaac aatagagttt
     6841 ggtgaaataa tagaatctgc aatagagaga gttaagttaa attactcatt atctttaaaa
     6901 aaagttataa atgcaactgg tacagttatt catacaaatt taggaaggtc tttacttagt
     6961 gaagatataa aagatgaatt atggtgtgct gcttcaaggt attcaaactt agaatacgat
     7021 ttggataatg gagaaagagg ttcaagatac tctcatttaa ctaatacaat aaaaagactt
     7081 actggagcag aagatgtact agttgtaaat aataatgcag cggcagtact tcttgtactt
     7141 agcacaatgg ctaaaggagg agaagcaatt gtatctcgtg gagaattagt tgaagtaggt
     7201 ggttcattta ggattccaag tataatggct cttagtggtg cagaattagt tgaagttggt
     7261 tcaactaata aaactcattt gaaagattac aaggaggcca ttacagaaga tacaaatgta
     7321 ttgatgaagg ttcatacaag taattataga ataatgggat tcacagagag tgtttctata
     7381 gaagaattgg ttaatttagg taaaaaatat aaactacctg taatagaaga cttaggaagt
     7441 ggagttttta tagatttatc aaaatatggt ttatcttatg aaccaactgt attggattct
     7501 attcgacaag gtgcagatgt agtaactttt agtggagata agatgcttgg aggtccacaa
     7561 gcaggtatta tagttgggaa aaaagagtat atagaaaaaa tgaagaaaaa tcaacttact
     7621 agagcactta gagttgataa gcttacaata tgtgctcttg aagcaacatt gagaatgtat
     7681 ttagatgaga caaaagctat agaaaatata cctactttaa aaatgttgac atataaaatt
     7741 gaagaattag aagtaaaagc aaataaacta tttgaaaaaa taactgcttt aaacttaaat
     7801 gcaaacataa atatagaaga tggattttca caggtgggag gagggtctat gcctcttgaa
     7861 actataagta caaaggtaat atcaataact cctgaatata tgaatgtttc atctttagaa
     7921 aaaaaactaa gacttagtga agcacatata atagccagag tgtatgataa taaatatgta
     7981 ctagatgtaa gaactatatt tgatgatgaa tttgatgtaa tagttgagga attgagaaaa
     8041 gcatttaatt aaataaattt gagttaataa atattaaatt tagaagtata agtatgaagt
     8101 tttaattaaa aaatagctac acacacttgt gactttagtc atagcaggtt tagagggggc
     8161 aaaaaatgaa aaatgtagta attggaacag caggtcatat tgaccacgga aaaactactc
     8221 ttataaaagc attgactgga agagagactg atacgttaga tgaagaaaaa aagagaggta
     8281 tttcaataaa cttaggattt acttattttg atttaccaaa caatagaaga gctggaattg
     8341 ttgatgtccc aggacacgaa aaatttataa aaaacatgtt agctggagca tcaggaattg
     8401 atatagtttt acttgttgta gcagcagatg aaggggttat gccacagaca gttgagcatt
     8461 tagatattct aagcttttta aatatcaaaa atggaatagt ggttctaact aagtctgata
     8521 ctgtagatga tgaatttaga gagctagtaa aagaagatat ttgtgaaaaa ataaaaggaa
     8581 cttttcttga agatgctgaa ataattgaag ttgattctat atctaaaaga ggtatagaca
     8641 ctcttattga aaaaatagat aaaatgagtg atgatataga agataaaaat gaaaactctc
     8701 cagcaagact taatatagac agagtatttt caataaaagg gtttggtact gtaataacag
     8761 gcactttaat agaaggaaag ataagtatag aagatgattt ggttatatat ccacaggtat
     8821 taaaaacaaa aataagaagt atacaagttc atggagaaaa taaagataca gcttatgcag
     8881 gtcaaagaac agcaatcaat atatcaaatg taaaagtgga tgatataaaa agaggagatg
     8941 ttttagctgc accaaactca ttggaggaat caatgatgtt agatgtaaaa ttatcattgg
     9001 taaatcattt agataaagga ttaaagcact gggatagatt aagattgtat catggaacta
     9061 gagagatact ttgtagagca gttccactag ataaagaatt aattgaaagt ggagaaagtg
     9121 gctatgttca gttgagactt gaagaaagca tagtatctaa gaaaggtgat acgtttgtag
     9181 tgagacgtta ttctccaatg gaaactatag gtggaggtgt aataattgac ccatctccta
     9241 aaaaacataa gaaatttgat gaaaaagtta tagaagcatt aaaaataaaa gaaaaaggcg
     9301 aactaaaaga tataatagaa gagtacttaa aaaggaattt aaaaaattat ccaaatataa
     9361 aagaaataat gagctatagt ggtgctcatg aagaagatgt aaaaagagcg cttgaaacac
     9421 ttatatcaga agataaagta tttattatag gaaatatgta tatgcatata aatcaataca
     9481 ataaattaaa agaaaataca ataaagcttt taagtgagta ccataagaag tataggctta
     9541 gaaaagggat actaaaagaa gaagtaaggt ctaagataga gagtaatttt aagactaggg
     9601 agatggatat tttattagaa aaattgtcta cagaaaatgc tataaaaata gaaaataata
     9661 tagtatctct attagatttt gaggttattt taaatgataa gcaaaaagaa atagcaaaaa
     9721 aaatagaaaa aagattaaaa tcatgtggag tttcttctat actgacaata gatgaagttt
     9781 ctgaaggaaa tcataactat gctgaagtat tggagtcaat gataggtaat aaagtagaga
     9841 aattagatga tttgtatata atggacaaag atatttatga aaatgcaaag aacattttga
     9901 taaattatat aaaagaaaat aaagaaataa cattaggaga atacagagat ttgattgatt
     9961 ctagcagaaa gaattgcatg attatattgg aaaacttcga tagaaacaaa ataactaaaa
    10021 gagtagaaaa caaaagaatt ttgttttagc agaaaatgtc tgtttttgct ttaaggtata
    10081 aagaaggaat tataatgtcg agtatcgtat aattaattag tataaaatat taattaagag
    10141 gaagtgtaaa gtattgaaga atataatacc tgatgtaaaa ccagatataa aagaaagaga
    10201 agacatgagc cttttattta taataatggg ctcagtactt ttattattta ttataaaaga
    10261 tatagagaaa tatgaaactt tattacatgc catatatacg atatttttag tagctacagg
    10321 agtaattata tttaatacta caaaatttaa agtagattca ttttctacat ttttaggatt
    10381 aatttttata gctactggag tattggagtg tatatattta tttaattcct tggggattaa
    10441 aactaagtct ataatggaga ttaacatcac aatatcagca ataacagatt tatttcctat
    10501 actgggtgta tacttaagtt ttaaatttgt taaggataac aaacaaattt attcaagtgt
    10561 aattttattt atagcaactg ctacattaac aataacatct ttattcataa tatcaggaat
    10621 ttgtgactat ctgggaaatg gtaaatcaca atatgcttta ggagttataa tatctatatt
    10681 cataataatt atttctatca ttagtggaat tgaattaaat tcaagttcaa ataaaggtaa
    10741 atgggaatat gatgaaaaaa agatttttaa cagaataata gtaataataa tcttatcaag
    10801 agttccaaat ctattgcata tagttattga taatagaaat gtagagagga tattatcaca
    10861 aataataata aatgtagcct tatattattt atataattac atagtatcta agaatattaa
    10921 gaaaacagca ctggaattgc atgatacgaa tgaagaatta acaaaaaaaa cggaaagttt
    10981 aaaagaaaag aataaaaaat taatctatga gacagaaaag atagaggaac ttaagggaat
    11041 acttatgaaa agagaatcta ggctacaatc tacattagat gtagcagtaa atagcatagt
    11101 agtatttagc aaagatggag aaattacata tgcaaataag agctttagaa atacttttgg
    11161 agactataaa gaacaagata aattgactga taaaataaaa aacttcaatg atttgataga
    11221 aagcattcat tatgtgtttg ttcatgagaa gaatgcagag aaacttatta gtacaagcga
    11281 taataaagtt tatcaagcta tatttgcacc tttaattatt gcttcacaaa cagaaggagt
    11341 attgtgtgta ctagaagata agactaagaa aaaggagtac gaacgcaaac ttattgaagc
    11401 aaataagaga agtaaagatt ttttagaaag tattggagat ggcatagtag tgcttgaagg
    11461 taataacaaa atatatgtta ataatgcatg tagggaaatt tttaaaaata atttagagag
    11521 tattgatttt tcattagtat gtaaaagtga agaaagtata gaaaaaagat atgtaataga
    11581 tggtgaagta aagtatgtag agatgagttt ttctcagtat actaatgctg gtactaataa
    11641 aactattatt gtaatacgtg atacaacctc tcgaaaaata tctcaaataa aacttgagga
    11701 aagtcaatca tcatattcaa gatttataga tatattacct gatggcatat gcttattaaa
    11761 agaagattta agtataaact atgcaaataa gtcgttttta gatatgctag cttttacaaa
    11821 catagatgat ataaaagatt ctaatattaa actattaatg aatgcgaata ctgaagaaaa
    11881 aatgaaattt acggataata tggctaaagt tttaaatgaa aatagaagta tacttttact
    11941 ggaatatgaa ttgataaata gttatgatga tatagtagaa gtagaattaa gtgcattacc
    12001 ttttgctata tacaatacta gatacataat gcttattata aaagatttag tacataagaa
    12061 atattccgag caagcagaaa aagaactgct tgaaagattt aaaacagata aaatcaaaac
    12121 ggagtttttt gcaaatatgt ctcatgaact aaaaactcca ctaaatgtta tatctagttc
    12181 aaatcaactg gtagattctt tctatagaaa tgaaaagata aaagattaca ataacaatat
    12241 taaatctcat gtggatttag ttagacaaag ttcttatagg cttcaaagat taataaataa
    12301 tattatagat ttaacaaaga tggagtctgg gttctataca ctaaaacttg cgaaatacaa
    12361 tattgtatct gtaatagaag acttatttat gaatatagaa gagtatgcac ttagaaaaga
    12421 cataaaaatt ctttttgata cagaccttga agaaattaat gtttatatag acaaggttga
    12481 aattgagaga attatgttga atctattgtc aaattgtatt aagtttactg ataatggagg
    12541 ctggatatat gtaagtattc attataaaat agataaagta atcataagtg taaaagacac
    12601 tggagttgga ataccacaag ataaattaga attgattttt gaagaatttt cacaagttga
    12661 taagacttta tccagaaata ctgaaggaag tggaatagga cttgctatag ttaagaatct
    12721 agtaagtctt catggtggag atatagaagt agtaagtgaa gtgaataaag gcacagagtt
    12781 cttagtttca cttccaatga agggatttag tagtgaacat tacacagaag ataagagaat
    12841 atataatatt caagaaaaaa taaaaataga attttctgat atatattatt aaaaatgatt
    12901 atgagcaaaa ataaaataaa aatactattt gtttaaatat ttaagttgta agaaataggc
    12961 tatcataagg taaattatta tgatagccta tttttagcta ggttttatat ctatatgtga
    13021 gaatatataa tctctaaatt accttcaaaa ctaaaatctc caaagtcgtt tggtagtata
    13081 aaatgcatac ccttttttaa attatattct acagaattgt gtataagttt tccatttcca
    13141 tccaacacac tgtataaaga aaaaggtgta ttgtgtgtaa aattacattt tccaaaaaca
    13201 tctaatttat aaacactaaa aaattcgttt gacacaaatg ttgtgcattt aaagtctgat
    13261 gttgatacga ttttatagtc agtatcgaaa tttatatgag gaacattcgt aacatctatt
    13321 gatttttgaa catgaagttc acgcttatta cctgaattgt cagttctatc ataatcatag
    13381 actctataag tagtatctga gttttgttga gtttctaaaa tcaatgttcc tttgcatata
    13441 gcatgtatag taccacttgg aacatagaaa aagtctccct tttttatgtt aacctttcta
    13501 agtagtttgt cccattcatt attatttacc atatttacaa attctttatg agattttgca
    13561 ttatgaccta ttataatttc tgcatcatca gaacaatcta ttacatacca acactctgtc
    13621 ttacctaact ctccattttc atttttttta gcatattcat catttggatg aacttgaact
    13681 gataaattat catttgcatc caatattttt gtaagtaaag gaaacttatc accaggtgtg
    13741 tttccaaata attctctatt tttattccac aattcagata gttttttacc tttatatttt
    13801 ccattttcta ttaagcaatc tccattttta tgagagctta tagcccaaca ttcaccagtt
    13861 gttggcgaat ctatttcata attaaattta tctttaagtg cagtaccacc ccatattcta
    13921 tccataaaaa taggttttaa aaataatggt tccataattc tgttcctcct atgatattaa
    13981 atttatccta caaataatta tatataaata gattataaaa tcaatagtaa aaaagttttg
    14041 tcccttgaaa tgacataaag tataaggagt tattaaataa gataaactat gatatataat
    14101 ataaagttct aaattggagt gataattatg gagttgttta aggcagaaag caaaaaatat
    14161 gaaaaagaat ttttaaaatt attggagcaa tgggttagta taccttcatt ttatgataga
    14221 aaaactgtat ctaaagatat gccgtttgga aaaggagtat atgatgcatt aaactggttt
    14281 gaaaatctag gtagagaaaa caatttcaaa gtaaaaaata tagataatca tgctgttcaa
    14341 attgaatatg gaaatggtaa agagtatgta gatatatttg gacattgtga tgttgtaaat
    14401 ccaggtgaag gatgggatag tgaaccattt aaactaaata ttataggtga taaattagtt
    14461 gctagaggtg tatctgataa taaagggcct atgatagtga attttttagc actaaaaatg
    14521 ataaaggatt tagatattaa tttaaaaaga aaggttagat taatagctgg gggaaatgaa
    14581 gagagtggat ttaaatgtat aaagcattat tatagcaaag agctatatgg tgtatgtgga
    14641 tttactccag atgctaaatt tcctgtatta aatggagaaa aaggtggagc aataataaag
    14701 ttaatttcaa atatagacga taaatcttta tatataagtg gtggaataga gtttaataca
    14761 ataccagata aagtttatat aaagaatgta gaaaaattgg gtaaagataa tatatgtttt
    14821 gatattaaca atatatcgat aaattatgat aatggcaatt atattgtgta tggaaaaggt
    14881 ggtcattcat ctaaacccga aaaatcaata aatcctatat tagctacaat taaacttttg
    14941 tcagaaaata ttgatgaaaa atggacaaaa gatttatata agttgataaa tcaagataat
    15001 ataaatggaa acttatttgg tttaaatatt gaggggaaat gtgggatttt atcaatggtg
    15061 ccaacaataa taaatatagt agatggaaaa ttagaagtag ttttaagcgt tagatatcca
    15121 gaaatattaa cccttgaaga tataataaaa aaatttaatt tgtatatgga gcaaaataat
    15181 attaataaat ttgagcttat tggggaaaat ttaaagcaag caaattatat agatagaaat
    15241 tcaaaacttg taagaagttt gcatgatata tatataaagt attctggaga tttaaaaaat
    15301 gatgttagag taactagtgc aggaagttat gcttctgaaa tgaataattc agtaatattt
    15361 ggttgtgaat ttcctgatgg tagctttggc aatgtccata gtgcgaatga atttgctagc
    15421 ttagatagat ttataactgc aataggtata tatgcagagg caataattac attatgtaat
    15481 aaaatataaa tacaaaatgt cccttatata gacatattat atatacaatt aaaattttac
    15541 ttgatatata attgaaatca aagagaaggt gatacaatga taactaagaa acatgcaata
    15601 atagtaaaga gtttaagcaa taaagatgga tatatgacat caaacgaatt agctatcaaa
    15661 ttggatgtgt ctactaaaac tattaaaaga tatatagcag atttaaatag tgttttaagc
    15721 aagtatgatt tggtaatagc ttcttctaga ggtataggat acaagttaag cggttctaaa
    15781 aacaatatag ccagagctat taaagaagca aataaatata ttgatggttt tttagatgac
    15841 tctgaagagt caagaatgtc aaatataata tgtatgctta ttaatagaaa ttatatgagt
    15901 atagaagcaa tggcagaaga attaaatcta agtatagccg ctataaataa attgtctagt
    15961 aagcttaaaa gaaaacttga aaagtatgac ttagttataa aatctaaacc ttactatgga
    16021 agctatatac atggtgaaga aattaatata aggcaattaa ttactgacta tgcaataaaa
    16081 ttagatgaaa aaagtaaagt gaaggtgttt ttagatgaca ttagtgaaaa cgatatacac
    16141 tgtatagaaa gtattttaga aaagcactta agagaaaaag atacaataat ttcagataaa
    16201 gattttaatc ttttgctttc aaaaataatc gtttctgtat ttaggagtaa gagaggtcat
    16261 agtaacaata taaacttaat ggatacaagt tatagatttc ataactacta ctttatagaa
    16321 aatctaatga aagaaatatc tgataaaatt ggatttaaac ttattgaaga tgaagtaatt
    16381 tatatttcaa attattctgg tgtaatagcc tataaaggta cacaaggaag aaaaaacaca
    16441 agtgaaattg aggaacgaat aagtagtgtt ataagtagtg cacttcaaga tatattcctt
    16501 atttcaggta gtgattatac aaaagatgat gagtttatgg ttgcaatctc tgaccatata
    16561 aagagatttt taaatagagc tagagctaat gtaaaatcaa ataatccttt acttcatcaa
    16621 ataaaagaga agtttcctat agcttttaat ttagccgtgt ttttatcaaa taaactagaa
    16681 actgagttta atttaaaact tgatgaagat gagcttggat atatagctat ccactttgct
    16741 gcttcaaatg aaagaatgaa aaaaaatact agtaagaaaa tttgtatagt ttgtcattat
    16801 gggataggaa caggacaact tatttctgaa aagctaaaac agaatataag tgatttaagt
    16861 gttgtaggtg tttatccagt tagatatctc gatatggcta taagtcaaga tgttgatttg
    16921 atagtatcta ccgttgaatt aaaaggatat gaaaaacctg tcttatatat agaaaatata
    16981 tttgatgata gcttaattga aaacgtaaac aaagcctttt atgaaaagga agaaagaaga
    17041 aagataataa gcaacatgtt tgatgaaaaa gcttttttca gtataaaggc tagtactaaa
    17101 gaagaggtca taatttcact ttcaaataaa ctaaaagaaa gagattttat agaggaaggt
    17161 tctataaaaa gtataataga tagagaaaat atttcatcaa ctgaaatagg aaatctagta
    17221 gcaatacctc acacaatagt gaagggagat aaaaaatcta taataggagt tggaatactt
    17281 gaaaatccaa taatttggga taagcaagag gttcagctag tatttatggt attttttaat
    17341 actaaagaaa aacataattt ctcgatattt aaataccttt ataattttat aaaagatgaa
    17401 ggaggagtaa gaggaattat taaaatatgt gactttaata aattaatggc actgataggt
    17461 aattaaggag gaaattaata atgggaacaa acatatttaa caaagaatat gtatttttaa
    17521 atgttgatgc taagtcaaag gttgaagttt taaaatttat atcaaaaaaa gctaaagact
    17581 taaacttggc agaagatgag agcttagtat atgaaggttt aatggctaga gaagaacaat
    17641 ttactacaaa tttaggagaa tctatagcaa tacctcacac taaaaatgat gcaattgaaa
    17701 atccagcagt agtagtattg aagtttaatg aagatgttgt ttggaatgaa ggtgaggata
    17761 aggttaaatt agcaataagc ttacttatgc caggaaaaag taaagagaat attcatctta
    17821 aattattatc atctctttca agaaaattaa taaataaaga atttaaagat tctcttttaa
    17881 aaagtgataa tgttgaagaa atatctaact taataaatga agctttaggt ttataattaa
    17941 acactaaggt tataaaataa aaaaataatt aactaaaaat aggaggaatt tgaaatgaat
    18001 aaaaaattag tagcattatg tgcttgtcca atgggactag ctcacacttt catggcagca
    18061 gaagcaatag agcaagcagc aaaggcatta ggttatgaag caaaagttga aactcaaggt
    18121 gctgatggtg tacaaaatga gttaacaaga gatgatatat taggagcaac tatgataata
    18181 catgcagtag caataacacc agaaggtatg gaaagatttg atggttgtga agtttatgaa
    18241 gttgaattac aagaagctat aaaaaatgca gaaggtgtta taaaagaaat agaagaagat
    18301 ttaggaatat aataaattta ttgtggagga aatagaatgg caattaagaa aaaagttata
    18361 aacagttcag gtgcttctac aacaggagga aataatgtag gaaaatctac tccatctagt
    18421 ccaactcaat caaagggaaa tggaaaatgg aaagaaataa gtaaacatat aatgacaggt
    18481 atatcatata tgataccagt tttagtaatg ggtggactta taggggcctt atctcaatta
    18541 ataccatatg ctatattagg acttgaccca tctgtaggta tagttgatgc tatgaactca
    18601 ggagaattta cagggttcaa gcttagtctt ttaaatatag cacaattaat gtcaaatttc
    18661 ggatttactt tgtttggatt tgcgatacca ctgtttgcag cattttgtgc aaactctata
    18721 ggtggaaaaa cagctttaat tgcaggattt ataggtggat atatagctaa taaacctgta
    18781 ggtgtaccac aattcgttga tggacaatgg actgaagttg ttccagttgc atcaggattt
    18841 ttaggtgcaa tactaatagc atttataata ggatattttg taaaatggtt aaataaatct
    18901 ataaaagttt ctcataactg gttagctttt aaaacaacat ttttaatacc attaatagca
    18961 tcattagctt gtatggtatt gatgatattt ataataactc catttggtgg attaattaat
    19021 gaaagtatga aaaacttctt aacagcagca ggagcagctg gagaatatgt atatgcaaca
    19081 gctttggcag cagctacagc attcgactta ggaggaccaa taaataaagc agcaggattc
    19141 gttgcacttg gtttaactac agagaatgta cttccaataa cagcaagaac aatagctata
    19201 gttgttcctc caattggatt aggattaaca acattattag ataaaagatt agtaggaaga
    19261 agagtatatg atagacaatt ctatcaagca ggaaaaactt ctatattctt atcatttatg
    19321 ggtatatctg aaggagcaat accatttgca cttgagagac caggttttgt tataccttta
    19381 aacatagttg gaagtgtaat aggagctatt acaggtataa ttctaggagc aattcaatgg
    19441 ttcccagagt cagctatatg ggcatggcct ctagtagata acttatttgg atatataata
    19501 ggtatagcag ttggtgctac attcatagca gtaggaaata tattctatag aaataaatta
    19561 ataaaagatg gtaaacttgt tgtagattat atcgactagt atttaatttt tactatctat
    19621 aaataacttt ggctggtaat atggttttaa tcaatattac cagccttacc tatatgatta
    19681 attaaaggcg gtggagataa gatatggata agatagaaaa agtaagagag tattttagag
    19741 aatatgatat agatggattt cttataaata gtcctacaaa caagttttat gttggaaatt
    19801 tatttagctc ttcaggttat gtttttataa cgaaagaatc acaatatata atagtagatt
    19861 ttagatattt tgaagaaatt aaaagaaaat ctagtttgtt taatgtagta ttaatggaca
    19921 aaactagaac tcattttgat ataattaatg atatttgtag agagcagaat ataaaggaaa
    19981 ttggatttga gggaaatgaa gtatcttttg atttatatag aagtatgagt aataagttaa
    20041 gtgcaacctt aaaatcagtt gatttatcta cattaaggga aacaaaaaat gaagacgaaa
    20101 taaaatatat aaaaaaagct tgtgaaatag ttgatgctac attttatcat atagtagact
    20161 ttataaaggt tggtatgact gaaaagcaag ttgaaaatga aattgttaga attataaaag
    20221 atttaggtgg tcaaaaagaa tctttcgata caatagttgc atctgggctt agaggagcat
    20281 tacctcatgg aaaagcaagt gaaaaagtta tagagtatgg agattttgtt acctttgact
    20341 ttggtgctaa gtataataat tattgttcag atattaccag aaccatttgt atgggtacta
    20401 taaataaaga gttagaagaa atatataata tagtaagaaa agctaatgaa gagtgtataa
    20461 gggttttaag accgggaatg actactggag agatagataa agttgcaaga gacataattg
    20521 gttcatatgg atatgccaat aattttggtc ataatctggg tcatggtgta ggaattatgg
    20581 ttcatgaata tcctgcatta gctcctgaat caaatgaagt attaaaagaa ggaatgattg
    20641 taactataga gcctggtata tatgttccaa gtcttggagg cgtaagaata gaagatgatg
    20701 tacttataac acaagatgga tgtatgagat tgactacatc tactaaggat ttaatagttg
    20761 taagctaaaa ataaattaga taaatatttt ataacaataa gaaaagaaga ggtaaattta
    20821 aatgtataat tttgatgata aaccagatag agtaagtgaa aagtgtagaa aatgggattt
    20881 aaatattata agagataaat ttggagatat tagagaagat tttgtaccaa tgtggattgc
    20941 tgatatggat tttaaaatac caacagaaat agaaaacaaa tttattgaag ctgtaagaag
    21001 aggtgtattt ggatatacat actgttatga tgaattttat gattcagtta ttggttggca
    21061 aaaaaatatg catgaggtaa atgtagaaaa agaatggatt acattaactt atggaactgt
    21121 atcaacactt cattatgtag tacaagcttt ttgtaagaaa ggtgatagta taatattaaa
    21181 cacaccagtt tatgacccct ttgaatcatc agcaaaaaaa caaggtgtaa atgtaatttg
    21241 taatacatta gatgttgtaa atgatagata ttatataaat tttgataaat tagaagttca
    21301 aattaaagaa aataagccta aattaatgat gttttgtaca ccacataatc catctggaag
    21361 gatatggaca attgaagaaa tgacaagggt agcaactata tgtaaagaaa ataatgttat
    21421 tttggttgca gatgaagtac atgcagagca tattcattat ggtaaattta attcaatact
    21481 taagattgga aaagagcttt tagaaaatgt tattctattg acatcaccaa ataaaggatt
    21541 taatcttggt ggattaaaga cttcttattc aatagttata aataaggata taagggacaa
    21601 atttagaaat aaattaaagc aaaattctat aacttctcca aatgtgtttg gaataatagg
    21661 attgataacc gcttataatg aatgtcatga gtggttaagt ggtgtaaatg aatatataaa
    21721 atcaaattat gaacttttag aaacttgggt aaataaatat aataagataa aattgatgaa
    21781 aatggaatca tcttacttag cttggatgga tataagtgga ttaggtatta gtgcaagtga
    21841 gtttacagat aaattggcta tagatacagg agtattatta gaagatggga gtcattttgt
    21901 aaaagatggt gagaagtatg ttagaataaa tttaggaact caaaaggaaa atgtaattga
    21961 agctttaaat agaatggatt tatttttaaa gtctttataa aaatttggtt gaattagtat
    22021 tttatataaa tttaaactat taatatggta tatacataag tagaatttgt tttagattac
    22081 ttaagtatat accatatttt atataaaaat taaatttgca ttagtcatgt gataactatt
    22141 taaattcata tgtattttgt atgtataata gatataatta ttatttaaac ttattgttta
    22201 tttaattttt caatagaaaa agatttatct gtaagtttaa aattatcttt tataccctta
    22261 gttaagtata ctttcttatc cttagttatg aatatagcat ctacattatc aattttatta
    22321 attaaattca tacctttttc aagaccaaga ccaaatgtag aagtggataa ggcatcacaa
    22381 ttaatagatt tatcagagat tattgtaaca ctacttaaat tgttttcaaa aggataccct
    22441 gtaaatggat ttaacatatg atgatatatt tttccgtctt tttcgataaa tctttcataa
    22501 attccagaag taacaacaga cttatttgtg gtttctatgt ttcctatgga ttcaccattt
    22561 ttactagttg ggtcttgaat acctatttta aaaggtttat catttttatc attttctaaa
    22621 gtaaatatat ttcctcctaa gtttactaaa ccttttttta catcctctga ttttaaatag
    22681 gctacaattt tatcagcagc atatccttta gctatggcac ctaaatcaat tttcatattt
    22741 tctttggtta attttataga tttatttttt tcatccaata tgatgttctt ataatcaata
    22801 agaggcagaa gtttttgtat ctcagaatca cttggaactt tagctttatc tgttccaata
    22861 gcccataaat ttgataatgg accaattgtt atgtcaaaat atccatctga taatttggaa
    22921 tattgtatag cttcttttac gacgaaaaat gtatcatcag aaactctgac aaatttttta
    22981 ccagcattat catttatttt agaaacatca cttcctggta tatgagtgct cattttgttg
    23041 tcaatatatc ttaaaattga atcacattca tttaagattt tatcactttt ggattctttt
    23101 acattaaata cagtaacttc gtttactgta ccaaggtagt agctagtttt actatattta
    23161 gtattctggc ttgaactcct aaaaaatgaa aataaaatta atgctaaaat caatatcaca
    23221 aaaataaatg tgattttttt cttattcatg gtatattcct cctatgaaat tgtgttatta
    23281 ttttattata acataatttc ataggaggac aaaaaatgaa gaaaaaagac attcttctta
    23341 ttttaggcat tttggttgtt attacagcat gttatgggat aataaatgtt ataaattcta
    23401 aaaatgctgg aaatattgag atttatgtag ataataaatt gtataaaact gtttcaataa
    23461 atgccaaaga agaatttaaa atagagaata gaggggggta taacatagta aagattcatg
    23521 ataaaggggt tgagatagta gatgcttcat gccctgataa ggtttgcgta catactggat
    23581 ttataaacaa gccaagtcaa agcatagttt gtatacctaa tagagtgagc ataaaaataa
    23641 atacaaatga aaagaatgat aatcaagaag atataatttc taaataaaat aataaaaata
    23701 attgaaagga attggtgata attatggtta acttaggaaa cgattgggat gaattattga
    23761 aagaagaatt tgaaaaagat tactacttaa acttaagaaa atttcttata gaagaatata
    23821 aaacacgcca aattttccca aatatgcaca atatatatga agctttaaaa cacacttcat
    23881 acaaagacac aaaagtctta atattaggac aagaccctta tcatggagat aatcaagctc
    23941 atggtttagc tttttcagtt caaccacaag tgaaaactcc accatctcta ttaaatatgt
    24001 ataaagaatt aaaagatgat ttaggttgtt ttatacctaa taatggatat ttaatgcctt
    24061 gggcagacca aggagtgtta cttttaaata ctgctcttac tgttagagcg catgaagcta
    24121 attctcataa gaacaaagga tgggaaattt tcactgatag agtaatatct attttaagtg
    24181 agcgtgaaga cccagttatt tttgtactat ggggaagtaa tgcacgtaaa aaagtagagt
    24241 tgattgatac aagtaaacat tatattttgg aagcacctca tccaagccca ttgtcagcaa
    24301 gtaaaggctt ttttggatgt aaacacttct ctaagataaa tgaaattttg aaaaagctag
    24361 gaaaagaacc tattaattgg cagatagaaa atatataaaa gtaaaaggta acaaaatgat
    24421 aacaaaacaa caataaacaa tgacattgtt gtaactaata gaaatatgct taaatagttg
    24481 aaaatactat gtatgcatat ttctattttt ttgtcaagtt gacaactctt gaaaaaatag
    24541 ttacaattta gttacacaaa aaacataaat aaaaaaagaa acttacaaag cagaatttta
    24601 tgaagcaaaa tatatagtgt aaaaaatctt agggaggaaa ttttatatga attttaaaaa
    24661 attgagtaat gctaaagtct cggtaatggc agttgtttta tcagttggaa tattatcggg
    24721 atactctgcg cttaataaac aagtaacatt agtagtaagg ggagaagaaa gaactatatc
    24781 aacatttagt tctaatgtag gagacctttt agaatctcaa aacattagct atgatgcaaa
    24841 tgatatagta actttagatg aaaatacaaa gttatctaat ggagataaaa tagaagtaat
    24901 tgatgtaaag gaaaaaacag ttgtagaaac taaagacatt ccatttgaag ttacagttgt
    24961 tgaagatggc tctttattaa agggtgattc taaggttaaa gaagaaggaa agtctggtaa
    25021 aagtgaatta atatataaag aaacttatca caatggaaag aaagtagaaa aggaatttgt
    25081 gaaaaaggtt gttaaagctg agccagtaaa taaggttgta agcaaaggga caaaggtaga
    25141 aatgcaagta gcctcatcaa ggggagaaag ttcaagaaga atggcttcat catcatctag
    25201 tagcagtagt agtaacaatg gtaaaaatat gagagtggta gcaactgcat atactggtca
    25261 cagtataact gcaacaggaa caaaacctaa gtggggaaca atagctgtag accctaaggt
    25321 tataccatat ggaactaaag tttatatacc tcaatttgac atggtgttta ctgcagaaga
    25381 ctgtggtgga gcaataaaag gaaataaaat tgatatctac atgaatgatt caaaaacagt
    25441 ttataactgg ggaagaaaaa ctatagatat acgtatatta aaatagttaa ataatttata
    25501 tttttactgg aaattatatt ttgattatat agagaagaaa ctctttatag attctaaaaa
    25561 tataatttcc agttttttat tttggatatg taataaaaca ggtaaatttt aaccatgtat
    25621 agataaaagc tatttaaatt ataatttact atcatataaa tctataaaaa ttgttgcaaa
    25681 gagtcaaaat atatgatatt atagatttcg ttgagaagga tttcattatg ggagtggatg
    25741 ggtgccggtg tgccctctag tcttcaaaac tagtatgagg ggttaagagc ctcttaggtg
    25801 ggttcgattc ccacgcattc ccgccaatat gtaaaaggta tcttttaatt aaaagatacc
    25861 tttttaaatt tattttaaga taatttattt ttaataatat ataatgtttt gtattatata
    25921 atgtaaattt tctaatacta ttatatattt agataaaaat ctaggagtgt ggaaagatgg
    25981 aaaataattt tacaacgaga actagttttc tagttggaga tgatgggata aaaaaactta
    26041 ataattcaaa tataatagta tttggagttg gaggagttgg tagttttact gttgaagctt
    26101 tagcaagagc tggagtaggc aatattacta ttgtagattt tgatgatgta gatataacca
    26161 atataaatag acaaatacca gccctacatt caactgtagg aagatacaaa gttgatgtca
    26221 tggaagaaag aatattagat ataaatccta atatcaatat aaaaaaaata agaagtcttt
    26281 ataataaaga tactagtgac gaaatactaa ctgaaagata tgattatgtt gtggatgcga
    26341 tagacatggt aagttccaaa atacatctaa ttgaaacttg tgaaaaaaaa ggattgaaaa
    26401 taataagttc tatgggtatg ggaaataaat tagacccaac taaaatagtc gttacagata
    26461 tacacaaaac aagtacttgt cctctagcta aagttatgag gaaagaactt agggatagag
    26521 gtatcaaaaa gttaaaagta gtatattcta ctgaacaacc aatagaactt aagaagaaag
    26581 taatgaatgg tagaaaagta acacctggta gtgtatcatt tgtcccatct gtaggaggtt
    26641 taataatcgc atctgtaata gtaaatgaac tattaggaca ataagtttta aatattttaa
    26701 tttataaatt ttatatacaa atttgaaggc tagaaggatg aatataaagc aattttatat
    26761 tcatactttt agcctagtta ttttatatta ccttatttta tgtagttgta gctttatttt
    26821 ttagattatg tatctatttc ataattatat tttgtaaata taatttctcc atcactataa
    26881 aaaacaatat ctcctattct gtctgttcta taaattgata catggttttg ttttagatta
    26941 ttaataacct ccctatgtgg atgaccatat tgatttttat atccacatga tattgcagct
    27001 atatctggac taattttttt tataaattct aaagatgaag aagaattact tccatgatga
    27061 cctattttta taaaattaat attatttaat ttaaaagaat gtaaaatttc tttttcattt
    27121 tcttcttcag catcacccat aaataaaaaa gaattatcat taaaagttaa tttgaaaact
    27181 atagaattta aattagattc ttcttgtata tatgatggac ttaatacata tatatttata
    27241 ttgttatcta tgtttaaaac atcattttta tagagatgct gaatagataa gtttttatcc
    27301 gtacaagaat ttattagatt ttgataagcc tcactatcag ttgattgttc aggcatatat
    27361 atggagttta cattaaattt ttttataata ttatcaagac ttcctatgtg gtctgaatct
    27421 ggatgagttg ctattataat atcaatagtt tttattcttt tttgtctaag ataactaata
    27481 attatatttt cgctatcttc atcacctcca tcaattaata tattcttatt tgtgggtgtt
    27541 tgaactaaaa tactatcacc ttgtccaaca tcaatcatat gtatagatag tagagatttt
    27601 ttgtcacagc cgattaaaag tgatattata ataagtattg ataaaaaaat tttatttctc
    27661 aataaaaaat cactccattc attacatgtt tatataaatt tatgatgggt aatataagat
    27721 atatatgact tattgtattt tatattttaa acaatataaa tgaaattaat accataataa
    27781 aattggagtt ttttatatat acaaaataaa atttttaaaa ttaagcaata aaaaaggtag
    27841 gaggatttta tgtttttcaa agatattgaa ctaaattcaa aaaaagagtt agacccatat
    27901 tttgatttag tagattatga agcttgtgag tactgtttta gtactttata tatgtggcaa
    27961 catgtttata agacaggata ttatataggt gaagattttg ccgttttagt tggggaatac
    28021 gaaggagata gcttctcaat attaccactt gctaaaaaag ataaacttcc agaagttgta
    28081 gattttgtcc ttgaatattt ttctaaaaat aataaaaaaa tatacttaag aggtattact
    28141 actgaagtag tagagttttt aaaagaaaaa tatccaggta gatttgagta tatagaagaa
    28201 agagatttat ttgactatat ttatgatgca gaaagtttaa gaactctagc tggtaaaaag
    28261 aatcaaaaga agagaaacca tataaactat ttcttaaaag agtatgcagg tagatatgaa
    28321 gctaaattat tagataaaga aaactttgat gagtgtttag tactaatgaa ggaatgggaa
    28381 agtaataagg aagaaaataa tgaatttgat gaaagtatgg atgatgaatt aataggtatc
    28441 aaaaagatat ttaaccatta tgacatatta aaagataagg taaaagtgtt tggtgtttat
    28501 gtagatggaa aattagaagc ttttagtata ggagagcttt taaatccaaa tatggcattg
    28561 attcatatag aaaaagctaa tcccgatata agaggtctgt atccatttat aaatcaacaa
    28621 tttttggtaa gtgaatttaa ggacgttgag tttgtaaata gagaagaaga tttaggtatt
    28681 gaaggattaa gaaaagctaa actttcttat catccttgca gatttgttga aaagtacagt
    28741 gttagggagg cttaatagac tatatggaga ttaggtatgc taaagaagaa gagatagaaa
    28801 gtataaaaga gatttggagt tattgtttta acgatacgga gagttttatg aagtactatt
    28861 ttaatgataa atataaatct gaaaatactg tagtagcttt agatgaagga aaaattattt
    28921 cttctcttca gctaaatcag tataagttat tactaaatag caaggtatat aatacatcat
    28981 atgtagtagg agtttctaca cttccagagg gaagaggagc tggatatatg aacaaagtaa
    29041 tgaaatttac tttaaacgaa ttgtataaaa aagggcaatt agtttctata ttgatgccta
    29101 ttgactatag actttataga agatttggat atgagcattg ttacgaccag attgaatata
    29161 ctattaacac agatgattta aaaaatttta agtcaagtgg aaaaatgata aagtctaatt
    29221 tatcacaaat tgatgatttg atacaaatag atagaacttt tttaaatgag gttaatggta
    29281 atgtgttaaa agatgaacat tattatgaaa acttatttaa agagattcaa agtgaagatg
    29341 gatttttata cattcatgaa ggaaatgaaa aagatggata tatagtttat tttcttcagg
    29401 aagataaaat gtttgtcaga gagttatttt ataaaaatat agatgccctt aaatcaatgc
    29461 ttaaatttat atataatcac aatactcaat gtaagatagt tactatatct actcctacaa
    29521 tagataagat aagatttata ttagacaacc ctaaagatag tgatataaaa ataaaacctt
    29581 ttatgatggg aagagttata aatgtaaaga agtttattga agatatagat attgagaatg
    29641 atattaatag ctcatttaat ttattaatag aagacaagtt tatagatgaa aataatggtc
    29701 tatttaaaat ttctatacag aataagaagg tatctgtaga gcagttagat aaaaaaggtg
    29761 cagaaaagcc tcaagaagat tttgatatta aattagatat aaatacctta acacagttat
    29821 cattttctta tatagatgta aatgaagcta ttttcttaaa tgatataaaa gatgtttcag
    29881 aagaaacact agaaacactt aattgtattt tttctaaaaa aaataactat ataaatgaat
    29941 atatataggt agagtacgtt aaaagatttt aaaaatcttt tgacgtattt ttttgtttag
    30001 ataacaattt atcttgttta aaaattttct atttgaaaat aatctaaata atatactttt
    30061 atatgggagt gattataatt caaataagaa gaccattatt aattatatta atgtttgtgg
    30121 tagctatatc atttatcatt acaaataaca caaaaaacga cgaagaactt aatgataaga
    30181 taataactat acaaggagtt gtcaaaggaa aaattgaaaa gaaaagatac aatcagtaca
    30241 aggtaggggt ttttctaata aatgattata caaaaaataa aaatttgaag attggtcaga
    30301 aagttaatat tacaggtaaa ttcaaaagtt tagataaaat gaagtatgat gattttgatt
    30361 atggaaggta tataaaaagt actggatata aaggtatagt gtatataaat aactataaaa
    30421 ttattgataa aaataaactt tactctttaa taggggaaat aaagttttat attagtaaaa
    30481 cttataggta cttatataag aaaaactcag attttataaa ttctatacta ttagcagaag
    30541 ttgaaaattt gactgaggaa caaaaagaaa tttttactag aactggaact agtcatgtaa
    30601 tatctatatc aggtcttcat acaggaatat tatgtgtaat tatatccttt ctattaagag
    30661 gtataaataa attgtataaa ttattaatac tgtcgatttt tataacttta tattgtataa
    30721 tggtaggtgc gtctccatcc ataattaggt ctatagcatt tgttatgatt ttttatttat
    30781 caatatttat tgatagaaag aaagatggaa tatcggcatt gtctctaatt gggataattt
    30841 taattatgaa taacccctat gttatatata atgtaagttt tcaattgtct tttttagcta
    30901 cactttctat attaatattc tataataaaa taaatagtat tataaaatta agtatggtat
    30961 cattgacaat atcatcaaat atattaactt tacctataat ttattataca tttaaaggta
    31021 taccattgct ttcaattata ggaaatttaa ttatagtacc atttgtggga gtaataatgt
    31081 atttaagtat tgcaagttta atcgtattta aagtaagtgt agtaatagct aaaattatat
    31141 ctttttttaa tagtacattg atagaaagta tatttttttt actagaaaaa atcagcaatt
    31201 taagttttgc atacattaat atagaaaatc ctaaatttta tattgttgtt atttattaca
    31261 taggagtgtt tttttacata ttttacatag aaggaaaaga gataaaggag caagagaatg
    31321 aattacaagg atattataag gaatgtaaaa gagaaaaact ttgaaaaaat gtatcttttt
    31381 tatggaagag agtattattt aattgagaat gcaataaaag catttaaaga tagtctaaac
    31441 gagggaatgc ttgattttaa cttagatata atagatggga aggaaatagt cttaaatcag
    31501 cttataagct ctatagaaac acttccattt atggatgata gaaagatagt aataataaaa
    31561 gactttgaac tattaaaagg aaagaaaaag aattttacag atagtgatga gaaatactta
    31621 atagagcatt tagacaatat tccagacacc acaactatag tttttgttgt atatggagat
    31681 gtagacaaaa gaaaatcatt agttaagaaa ataggaaaca atggaattgt ttttgattgt
    31741 gataagcttt ctgatatgga tttgtttaaa tggataaaaa aatcttttgc tttaaatgat
    31801 gttataatag ataattctca aatcatgtat tttattgaac aagaaggata cagagataag
    31861 agtagtgaaa agacattatc tgatttagaa aatgaaataa ataaaataag ttcatttgtt
    31921 ggtaaaggga ataatgtaac caatgatgta attgataaat tatctcaaaa aaaagttgag
    31981 aatgatatat tcaaattaat cgattatatt ggagaacaaa atgcttctaa tgcaatgaaa
    32041 atattaaatg atatgataca agaaggcgaa tctgtattag gtatattttc tatgatagca
    32101 agacaattta aaataattat gcaagtaaga cagttacaac ttgatggata ttcaacaaaa
    32161 cttatagctg ataaattaaa aatgcatcaa tttgttgtag gaaaagcatt aaagcaaact
    32221 aaaaattttt ctgatgatat aattgtggaa atattaaact atatattaga aagtgattac
    32281 aaaataaaga caggtcttat aagagatact ttagcagttg aaatgttggt tagtagatac
    32341 tgtaaaagag aagcaattta aaaaatctat atattaataa aaaataaata gcaagatatg
    32401 ttatagttca gagtagatgt aagctaaatt aagtttgata tacaatcaac tataatatgt
    32461 ctaactattt attttttatt gtataaattt acaaaataaa aaacccctac ataggggtta
    32521 ttatatataa gaaatataaa ctggtaatta agcgttcata ccgtttaatt tttgagctaa
    32581 tttagctact tttctagctg cagcattctt atgtatagtt cctttagaag ctacttggta
    32641 tattcttttt tgagcgtatt ggaacttagc aacagcatcc tctctgtttc cagctgcaac
    32701 agcatcttcg aatcttctta ttgcagtttt tatttgagat tttctaactc tatttaaagc
    32761 agttttcttt tcgataacac ttattctttt cttagctgat tttatatttg ccaccgtgtt
    32821 cacctccctg tattttttta tcatattcat cgtcaacata ttagatttta acagaaataa
    32881 atctaaaaat caaggattat tatttatata ttttaataat gttttttatt ttgatttttt
    32941 agactgtaaa ttgtgtataa ataatttgca atatcttgtt tttaaattgg tatattttgt
    33001 gttttaaata ttttgacata tataaattaa tattttattt ttgtaacaaa tattttgaga
    33061 taatacaaac tattaacata catttt
//
LOCUS       DACXMZ010000015        32726 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.015, whole genome shotgun sequence.
ACCESSION   DACXMZ010000015 DACXMZ010000000
VERSION     DACXMZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.015"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1063)
                     /locus_tag="JD016_02815"
     CDS             complement(<1..1063)
                     /locus_tag="JD016_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998562.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTCLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFM"
     gene            complement(1041..2117)
                     /locus_tag="JD016_02820"
     CDS             complement(1041..2117)
                     /locus_tag="JD016_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HAU4998563.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            complement(2181..3317)
                     /locus_tag="JD016_02825"
     CDS             complement(2181..3317)
                     /locus_tag="JD016_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAU4998564.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            complement(3318..4880)
                     /locus_tag="JD016_02830"
     CDS             complement(3318..4880)
                     /locus_tag="JD016_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998565.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            complement(4967..7726)
                     /locus_tag="JD016_02835"
     CDS             complement(4967..7726)
                     /locus_tag="JD016_02835"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HAU4998566.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            complement(7716..8345)
                     /locus_tag="JD016_02840"
     CDS             complement(7716..8345)
                     /locus_tag="JD016_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HAU4998567.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKNLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            complement(8431..9009)
                     /locus_tag="JD016_02845"
     CDS             complement(8431..9009)
                     /locus_tag="JD016_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HAU4998568.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            complement(9048..9626)
                     /locus_tag="JD016_02850"
     CDS             complement(9048..9626)
                     /locus_tag="JD016_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HAU4998569.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            9955..11391
                     /locus_tag="JD016_02855"
     CDS             9955..11391
                     /locus_tag="JD016_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HAU4998570.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKRSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLSNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKISPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            complement(11483..13357)
                     /gene="asnB"
                     /locus_tag="JD016_02860"
     CDS             complement(11483..13357)
                     /gene="asnB"
                     /locus_tag="JD016_02860"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAU4998571.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            13530..14234
                     /locus_tag="JD016_02865"
     CDS             13530..14234
                     /locus_tag="JD016_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAU4998572.1"
                     /translation="MKKKILIPVIMSLFIISQCITSFAFTPENNKFKVKPLPYAYDAL
                     EPYIDKETMKLHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVR
                     NNAGGAYNHKFFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWA
                     WLVATKDGKLSIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVV
                     NWNGALENYKNLKSQD"
     gene            14347..14883
                     /locus_tag="JD016_02870"
     CDS             14347..14883
                     /locus_tag="JD016_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAU4998573.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(15049..15669)
                     /locus_tag="JD016_02875"
     CDS             complement(15049..15669)
                     /locus_tag="JD016_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAU4998574.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(15983..18121)
                     /gene="vanT"
                     /locus_tag="JD016_02880"
     CDS             complement(15983..18121)
                     /gene="vanT"
                     /locus_tag="JD016_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HAU4998575.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(18139..18945)
                     /locus_tag="JD016_02885"
     CDS             complement(18139..18945)
                     /locus_tag="JD016_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAU4998576.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(18942..20042)
                     /gene="vanG"
                     /locus_tag="JD016_02890"
     CDS             complement(18942..20042)
                     /gene="vanG"
                     /locus_tag="JD016_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HAU4998577.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(20303..21445)
                     /locus_tag="JD016_02895"
     CDS             complement(20303..21445)
                     /locus_tag="JD016_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAU4998578.1"
                     /translation="MKSKNIKVNPDFTQVRNKIFMRTALMAFIAIIFIWILYSFILYG
                     KFSIWIVSFFENAFRMEYKAALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTK
                     YFSEINQGIDSLIKEDVGEVALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELI
                     VYLAHDLKTPLTSVIGYLTLLRDESQISEELRKKYLSISLDKAERLEDLINEFFEITR
                     FNLSNISLEYSTVSLTRMLEQLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVF
                     DNLLRNAVYYSFEDTTIEITAVQEDDYIKLKFINYSNTIPEEKLERIFEQFYRLDTSR
                     NSNSGRAGLGLAIAKEIVELHNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(21435..22136)
                     /locus_tag="JD016_02900"
     CDS             complement(21435..22136)
                     /locus_tag="JD016_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanS"
                     /protein_id="HAU4998579.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(22570..25101)
                     /locus_tag="JD016_02905"
     CDS             complement(22570..25101)
                     /locus_tag="JD016_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAU4998580.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKKALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNIKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(25209..25826)
                     /locus_tag="JD016_02910"
     CDS             complement(25209..25826)
                     /locus_tag="JD016_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998581.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIIKPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(26188..27072)
                     /locus_tag="JD016_02915"
     CDS             complement(26188..27072)
                     /locus_tag="JD016_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HAU4998582.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(27263..28513)
                     /locus_tag="JD016_02920"
     CDS             complement(27263..28513)
                     /locus_tag="JD016_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAU4998583.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYENSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVGYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(28735..29499)
                     /locus_tag="JD016_02925"
     CDS             complement(28735..29499)
                     /locus_tag="JD016_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4998584.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(29493..30218)
                     /locus_tag="JD016_02930"
     CDS             complement(29493..30218)
                     /locus_tag="JD016_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4998585.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(30220..30588)
                     /locus_tag="JD016_02935"
     CDS             complement(30220..30588)
                     /locus_tag="JD016_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU4998586.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(30764..32308)
                     /locus_tag="JD016_02940"
     CDS             complement(30764..32308)
                     /locus_tag="JD016_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HAU4998587.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT        10983 a         5692 c         3380 g        12671 t
ORIGIN      
        1 gcataaattt acttttaata ggataattat tcttatcaat attaataatt ggacttcttg
       61 ttgttgaatg ataatatata tcatattgat tttctttttc ttttgcaaac agcataggta
      121 tatacataaa ttcttcagta cctaaaaata gaatcttttc tttttcattt tttaaagatt
      181 ctttactggc catagttatt tttttacttt ctctcttaac gatttctttt aatctaaatt
      241 gttctcttct attaattcca aatcttcctg tatattttac atatttctta tttccaatat
      301 aatcttctaa atcaacataa atatgattta ctactaaatt tactttatca cttgaaatag
      361 tctctaattt agtaatacta taattatcgt tgtaatttga aataatatct tcatctatat
      421 aaaatttaaa gtttccttta aataagtata caaaatctat cttacaatct agctctcttt
      481 ctaaacaagt aactttactt aaattttcat cacttaccca attgagtatt gagcatatag
      541 tgtatttctt tttaggataa atgctttgaa ttttcctaat tatattcatg catgtattgg
      601 ctgtggtaat ctcatcatca accaatatta tctcatctcc aaatttaaag ttgttaagca
      661 taccaaagta taaattctgg tctgttgcat gggaatgttc ctctaaaaat tccagatttt
      721 ctaactcgtc aaactgctct ctagtagtat gaataaattc ataatctcca cataagtaat
      781 caaaaaaact atgcccaaca gctgtaccag tttcagcaaa agctataaca gtaccatttg
      841 attcttcctt atttttttca ttataaattg tagccattaa ttttcccaaa ttatccatct
      901 cacttggctt acatgctaaa tgtttaccga gtttctttga gataaacaaa aaacgtcttt
      961 tgctattatt tcttattcct acgtctaaaa aatcatttag ttctaaattc aatggattat
     1021 caataatgtc aatctcacaa ttaaaattta agcaattctt catagtctac tcctttattt
     1081 aatacaccat atattttaga tagaatcaat gtttttttag cccataaaaa atgtggattt
     1141 gtttcattca ttctctctcc taatacaccc ttgctcacac catacttact gtctgtacta
     1201 tccagtatat ccaaagcatc agtaaaatct tcatatgata ctgtagatag cgcttgtaca
     1261 atttgaattt gcattggatg aattacggtc tttccaatta atccatgata tttatccaat
     1321 aatatttctt ctatatactt atttcttata tctttattcg tcatatcaaa aaattctgat
     1381 actccaccac ttataggtat atctaactct ggtctttgaa gatatgtgat tatatctaat
     1441 ataaatgaag aacaaatcaa attattatat atagaaaagt ttttatctct ccttatacca
     1501 tacatcccta atatatcagt tatgcctact ctaatgctta atattcttga cttatgtctt
     1561 aaaatagcat tgtacaaatt agtaaaacta agttcttttt tttcattata tataaattct
     1621 tttgtctcta ctatcggaat aacatacata tcttgaagat tcatagaatc taaagcttct
     1681 atacaatttt caattaactc cgaatttgct ttaggaatta atattcctgt tataaaatga
     1741 ctatttttaa ttataatctc tttaattctc aacagttgct ccacatcttt tattcttaca
     1801 aaaattagtg gtattccatc tttattagtt atactttcat tgctaatatt ttttaaaatt
     1861 aatctaagat tctctatagc ctctaactct ccatttactc caacagcatc ttctaaacaa
     1921 atagctaatg gtctaactcc cttaacatcc cctataatac tcttataaat catattatat
     1981 tgagtagctg gaacgtataa aaaagcgccc agtgcatatt tcaacacatc tttttcagtc
     2041 ttagcactaa aatcttctgg ctccttcaaa aaaattctct ccaaatcatc tttgcagata
     2101 taatcgaaat atttcaatgt ctagcctcct catatacata gaaagggcca actttataca
     2161 aattagccct taatataata ctacttatta taaattatag tcgtatttaa ttcttccaat
     2221 ctctttgtat tatccttaat taaagctcta ttgttttctt ctattctcat agtttcatct
     2281 ataccagatt tgatagtatt gtacattttt tccaatgttt ctatttgaac agaacttgtt
     2341 ccagccattc tagctatttg tgcactttga gtacttacat tttgtgcatt tctaagcaat
     2401 aattcattag ttttgtcatc aagtgctttt aaagattttg cttgtaattc ttgcttttta
     2461 agtaatatag cttgagataa acattgttta aatactggta aagttacgac aaatgcacta
     2521 tttatttttc taattaaacc ataattatta tgttgcatac ctctaagcat aggtattgtt
     2581 tggatagcta tattttctgc tatccttaaa tcatagactc tttgatttaa catatcataa
     2641 acttttaata gttcttgata attgacaaca tccatttggt ctccactagt ctctgatttt
     2701 tgtttaaaga aaggtagtat ttcattatcc atttcttcta ttgccatttc tccagcaaca
     2761 atatattttt gaagttcatt gtaaaactca aagttttcat ttaacatagc acctatttgc
     2821 ttattagaaa gtgctatatc tctttcgtat tgttcaagct caatttgaat tttctcaact
     2881 tctcctccca atgtttcata cttttgaaac atcatttcaa tggcattatt agcttttttg
     2941 aaaagttttt gaacgaaatt tggttcctta gtttcttgaa aatcgtctaa atcaaacttg
     3001 tccattattt tagttaaatg aaccagcatt ttagaatttt cttcatttct atttaatttt
     3061 attgtattta aaaggctatc agaaactctt gctactcctt cagatgcttt cctaccaaac
     3121 tgtaaaatcg tatctggatt ttctacttct ataagagatg tcaatgcctc aacttcctta
     3181 gactttctca gttcattttt tttattattt gtatattcca ttatatcgaa gttttcttct
     3241 tgaactgctg taggcaaatt ctcagtaacc tctacagagt tcttatcttt tgaaaaacta
     3301 tttatattta tccccatcta aaatcgtctc ctctttccaa aaattttttt aagtaaaggt
     3361 atatcatttt caagcgaatt taatttaagg tcatatacca ttttatccaa tctgtgtaca
     3421 tcaatcaatt ggttatttat aacattctct atattgtttt ctcctcctgg acataatatg
     3481 ataatatcaa atccaatctt actaagtaaa agcataagaa aaactatttc tttatcaaat
     3541 acaatactct tgtacatgta tataatcaat tttgggttta taagtgagta atcgaaattg
     3601 ttcatcatat gaacaaattt cttatccatg tgaagtactg taaatagacc ttttatcttg
     3661 tcttctccac ttaaaccatt caaaaacata cttccttcta acaactcttc cactttctct
     3721 aaaatcataa tttgttgatt caatgctagt gttgatatgg aataatcttt attatttaaa
     3781 acagagtttt tatctattgc accttttgaa tccaataaaa aggataaact aaaagcttct
     3841 cttgtaaaag cttttgaaaa tctatctaca tctccattaa attcaataaa tgttgaactt
     3901 tccgaatctc ttaataaatc taaaaactta atatattcat tcttatcgtt atatattcca
     3961 ttaatcttag aaagaaaatt tggagcttct acaatagcat cattaaactt aaatcctggt
     4021 cttaatttaa gtggttgatt ccagtatatt gatatttcat caacagttga acttagaagt
     4081 agatttttta tttttctatc ttggagttgc caaggtttta taaatcctgc atcatttaac
     4141 aattcctcac ttatacgctt tgatgcttct gccccaacag taaaagcttt ttttacagaa
     4201 cttttatcta ttttttcccc taaaataacc ctttcttcaa aagaaatttt ttcttctatt
     4261 atgttggctt cttcaaactc tatattgtat ctttctgatt ttaaaatatc tatattactc
     4321 tttgaatttg gattaagata taaaacatca aaacctgcca agtataatat taagagtaaa
     4381 taaacttcat gtttttttat atctccataa aatattactt ttggagcttc atttttattt
     4441 atatctaaag caccgatata aatttttatc catgccatta ttttcactat aaaattttgt
     4501 ttaactctat cattagtaaa ttctttttcc tcttctttta acactctatc cattgcttgc
     4561 tttattttat aatcaatctc ttcaaatccc aaaatattta tatctacatc ttccatgcta
     4621 gttctatatt tttgaagtcc acttttaaaa taatttataa tatcaaaatc actaggattt
     4681 gctataacat cagtaaataa agcaatatct tttttgctat caatattttg ttttgtttta
     4741 aatatctcga gattgtacaa aacttcatct tctaaaccaa tatacgaaat aaaataactt
     4801 agatatccct ttgaaggagt gtaatttcct ctatctgatt ggctggagaa tattttttca
     4861 aatacctttg agtcacccat aactatcctc ccaatttaaa tgtttattca gaactgaccc
     4921 aaaattcata ttttgagtca gtctgttttc ttctaaaaaa tttgttttaa gcagtctttt
     4981 ttggttttac cagtcttatt atacatttta caacttcatt aaccacaatt acacttgctg
     5041 ctacacataa aacttttacc catgtcataa aatccattgc tacagagtta aagaaatctt
     5101 ggaatagctg agtaaatatt acttgtgcaa ttcctgtcaa cactattatt tgtagtgcta
     5161 atttgttatg tgtaaaatta gggaagatac tatctgcatt aaactctcta cagttaaatg
     5221 cattaaacag tgcactaaaa gcaaataacg caaacattac tgttccctta tgcgcttcat
     5281 caactcctaa gaaattaaat gtagattgta acattagtat tgctgttata taaaatgcat
     5341 ttaatactat tgtatacacc attgacttag aaattatatt ggcttttcta tcaacaggtt
     5401 ttctatttaa cacatggtcc ctaactggct ctaaacctaa tgctagtgct ggtggtccat
     5461 ccataatgat gtttacccat aataattgta ttgtagtaaa tggcatctct tgtcccataa
     5521 cttgagatat tatagccact aaaaatgcaa ttacatttac agttaattgg aattgaacga
     5581 atctttggaa gttatcataa attcctctac cccatttaat accttttaca atggtactaa
     5641 aactatcatc agttaaaatt atgtctgctg cgtttttaga tacctctgta ccagatatac
     5701 ccattgctat accaacatct gcttttgaaa gtgctggagc atcatttata ccatcacctg
     5761 ttactgcaac aacatctccg ctcttttgca gagctgaaac tattctcatt ttactatctg
     5821 gtttacttct tgccacgatt gaaatacttt gtatctcttg ttttaattct tcatcatcta
     5881 aagtatctat atatgaagct tcaaccactc tcatattatt ttttaatagg cctaactctt
     5941 cacctatagc tcttgccgta tttatattat ctcctgtaag catttttaca gaaacaccag
     6001 ctttatttgc tgtatttaca gactctataa catcttttct aagtgggtct cttataccaa
     6061 caaaaccact aaatactaaa ttgttttcca ttgcacttac attcatagca tcactttcag
     6121 cagttactgc tgcttcttct tcagcttgtg agatttcttt atatgcaaac cctaaagttc
     6181 tcattgattt tatttgcaat ttctttattt cttctaaaat ctcatgtttt atattttcag
     6241 ttaatggaac tatgtctttt cctctttgaa tatgtgaaca taattcaagt aagacttctg
     6301 gtgctccttt tgataatagt acatgatgat tatcttgacc tataacagaa gtcattctct
     6361 ttgaatctga agtaaaagga ttttgagcta ctatattaga attttttcta acttgtctat
     6421 aatctacgtt tttgtaatat aaaagcaatg cacattcagt agcacttcct aaatacttaa
     6481 tatcaccatc attgtgctct acatcagctg tagagtttat catacagttt tcttcaaaat
     6541 aactatttat ttcctcttct gaactttcaa tatatctgcc atcaacatat gcaacttcta
     6601 cagtcattct attttcagtt aatgtaccag ttttatctga acatattaca gaaacagaac
     6661 ctatcgtttc acaagcttct tttttggtaa ccaatgcatt tattttagcc attttttgca
     6721 ttgttattgc aagtgtcata tttatcatag ttggaagacc ttctggcact gctgcaacta
     6781 tcaacgcaac acatactgta aatgctgttt taactgggtc tattgattct aaaaacggta
     6841 atattccaga catatctaca tttaatacat tcttacttat catttggaaa accataaata
     6901 taaatagtaa tcctgctact gcactagaaa ctttggctat caaaccacct aaatttccaa
     6961 gttttatctg aagtggagtt tgctcatctg tttctgataa gttttgagca attttaccca
     7021 tttcactatt atctccaatt gaagttacaa ctagagctcc tctaccataa gctacaagtg
     7081 taccaccaaa caccatattt atctgttttg caggtattgg ttcctgtact actactgctc
     7141 ctttagattc tatctcttcc atagaaacta ctacatcaca ctttttagat acgtcatcag
     7201 attctcctgt taacatatct tctctaattt taaggtttat actttctata agtctaccat
     7261 cagcaggtac catgtcacca gtttcaatat atactatatc acctggtacc aattcactct
     7321 tagaaatttg atgtacttcc ccatctctca atacttttac ttctatgttt tcagtcaact
     7381 tagaaagagc ctctgctgct ttcttagaac gaccttctgt aatcatacct attgctatac
     7441 ctattgctat agcacctaca atccctattg catcatgagt ttccccaatt actgcactta
     7501 ctactgctgc aataatcaag ataactatca ttggttcact taaactttcc tttaaatcat
     7561 cccaaaaact accctcttct tttggagtaa attcattcaa accatatttt tctcttctag
     7621 tcttgacttc tgattctgat aaaccttttt caggattaac agataagtct tttgtaactt
     7681 cttctttatc tacattataa tatttgatat cattattaaa ttccatatgc acactcaatc
     7741 ctgtctaaat tttgcattac ttctttaaga cccgcatctt ttgttccttc accaattgct
     7801 ttaaattgcc aacttccatc ttttctatat atagctccta ctataagagt agttttttca
     7861 gaataattat cagatagatt gtattttgct atctctttct tattaccttg gtcttcaact
     7921 cttatatagg cattttctat cattccaaag tgttgttttc tatctacaca attgtatata
     7981 tttactacaa acaataattt atgaatattt gatggaactt tatccaaatt aactaggatt
     8041 tgttcatcat cacctgtacc ctcaccagtt agattgtctc ctgtatgcaa aactgattta
     8101 caggcacttt ttagatttcc aaaataaatt aaatttttta ttccacttaa tttaccttct
     8161 tgattaagca taaatacaga tgcatcacaa tctatatcag cttgtcctcc accaaataac
     8221 gatttaaaaa aacctccgcc agattgttgc acagggtccc atcctagacc tacaagtatg
     8281 ttactcagtc ctgggttaga tttttttaaa tctattttat caccttttga taaattaatc
     8341 gacatactaa acctccacaa aattttatat aatataacct atacattatt tgtataggtt
     8401 atttatattt aatatattat attgcttaaa ttaaacattt aagccatagt ttctacaaag
     8461 tgcttctaag ccaccttcaa atcctgctcc aactgcacta aacttccatt ctccattata
     8521 cttatatatc tcacatactg taattgcagt ttctatacta aattcttcac ctaagtcata
     8581 tcttaatatt tcttcatcat tttcagaatt taatactctt atatatgagt tgcttacttg
     8641 tccaaaattt tgtcttcttt cagcagcctc atatattgta actgttacag ctatcttttg
     8701 tatatgcttt ggcattgtct ctaaattcac taatatttcc tcatcatctc catcaccttc
     8761 accagttcta ttatcaccta agtgttcaac tgctccagat ggatgtttta agttattgta
     8821 gaatataaaa tcttcatctt tttcaactct ttgagattct ccaaccatga aaacacttac
     8881 atctaaatca taatcatagc cactatcaaa tgaatttgta tcccatccta gtccaagttt
     8941 tatattttta agacctggat ttcccttagt taaatcgatt ttttgtcctt tttttaatac
     9001 tatagccatg gaaaatatcc ccctttagtt tattgttata gttgaaatta taagtttatt
     9061 ccaaagtttc cacataatgc agcaagacca ccttcaaagc cagaacctag tgcattaaac
     9121 ttccattctc cattgtgctt atatatttca gctactacta tagctgtttc tatactaaaa
     9181 tcttctccta attcatactt tattaactct tcatttgtgt ctttatctac tattcttata
     9241 taagaattac ttacttgtcc aaaattttgt cttctttctg ttgcatcatg aattgtaaca
     9301 gtaaaagaaa tcttgcttat ttctccagga atcttagata aatcaacaac tatttgttca
     9361 tcatctccat caccttcgcc agttctgtta tctcctagat gctcaactgc tccagatgta
     9421 tgttttaaat tgttgtaaaa tacaaaatct ccatcattag taacacttcc acttgctcct
     9481 gttaaaaatg ctgctgtatc taaatcaaag tcaaatccac catcatactt gttagtatcc
     9541 catcctaaac ctactaaaat atttttaaga cctggatttc ctttagttaa atcaaccttt
     9601 tggccttttg ataatgttat acccataatt tatcgctccc ttgttaattt tagctaaaca
     9661 aatcttgcta attatttatc tttttcttta atcatattat aacatttttt attcatttgt
     9721 gaagaaaact tgcttgatat ttttaaaata taattaaaaa aatgtttata tttcttactt
     9781 tttacataga ttttacatac tttattaaat gtataaaagc tatgtttaat atgtaattat
     9841 atatcattta tataaacaag tacatatttt attttgaaac tacaaaatta ttgtactata
     9901 ttgttttaag ttgttttata atagattata tcatcttatt ataggagaaa aaatatgatt
     9961 tgtactatta aaccattaaa gatttcaata aaaaagtcta ctgaacctct aattgaattt
    10021 atatctgata ctacctataa aaataagcgt tcttcatata ttaaagaagt ctactttaca
    10081 agatatatag gtgttgatga ttcaattgat aaatacatag aaaaaattaa aaatatagat
    10141 acttattttt ttaataatcc tacaattccc tatattagat taagcaatct aaaaataaat
    10201 tttgacaaag aacaaacaga taagatgctt aaaattttta atatttatga tagttctaag
    10261 tttaacagtt gggatttata taatatcgac tttccatgta aaatagaaaa tgacacctta
    10321 aattggacta aaaagattgc atttaaaaat actttagatt tattttccac aagtatgcca
    10381 aattgtacat cctctatcat caaaaacttt gcagttaaac ttttgtgttg gatggactat
    10441 tttctaccta aattatttta taacaaggtg aagactacta tttcaccaaa gatagtatac
    10501 tttggaaata ttaaaaggca agaacttttt ttcttatatt ttttatcaca acttgggtgt
    10561 gatattttat atataaatcc aaataaagat attcttgatt tatatcctga atgttcaaaa
    10621 ttttctactc tatttgaact ttctaggaag actccaaata tattagaaat accttttgaa
    10681 aggcatatta caaatataaa ttacagtgaa aataccaata agatttctcc tactacgaat
    10741 aaacctaact ctaaaaatac acttaataac acaaatctaa catctaaaaa aattgtacaa
    10801 gatgaggaag tagaactttc ttatgaagac cttgcaaagt tatcagcttc tgttgttatg
    10861 attgttgtgt gcaataacga aaataaaccg tttaagagcg gttctggagt agtaataaat
    10921 aatgaaggtt atatacttac gaatcttcac gtagtcaatg atgggtattc atttttagtt
    10981 agatttgaaa atgatgataa agtttataca tcttaccaaa ttataaaata ccattcagat
    11041 tatgatttag ctgtgataaa ggttgataga aaatgcaaac caatacctgt taaagtcagt
    11101 aaaaaacctg tgagaggtca aaaaatagtt gcaattggaa gccctttagg tctttttaat
    11161 acagtttcag atggaattat ttcagctttt cgtgattttg aaacggttca aatgatacaa
    11221 tttacagctc caatctctag tggtagttca ggaggagctc ttttagatat gtttggaaac
    11281 ttactaggat tgatttccgc tggatatgat gatggacaaa acttaaattt agctgttgaa
    11341 agtaatcttg tcaaaatatt tgccaataat tttattgaga tagtaaacta atcaatcaaa
    11401 attctactta tcctattcca taataatcag gagggtgaaa acattgcatg cttttgccct
    11461 ctgattaata ttgaaaaaag tattaatact cgacattttt atgctcaata aataattgat
    11521 gccatagaca aaatgtaaat atagtccata tttttctcga attatctcta tgacctttaa
    11581 gatgcgtatc taaaagtttt attgcatatt ttttatctat aaattcatct acatttgaat
    11641 tataaatagt ttcctttaca atatccccta gagaatcttt taaccaaact cttattggcg
    11701 ttggaaatcc taattttttc ctttgaacaa catgttctgg gactatttct ctaaacgcct
    11761 ctctaagtaa aacttttgta ttattcttat ttattttttg ctccattctt aaattttttg
    11821 ctacatcaaa tacatcttta tataaaaatg gtactctaag ttcaacctgc tcagctgtag
    11881 aaagtttgct aattttttga agtatatcgc cttgaagcca tgtattcaca tctatatgtt
    11941 gcattttact tatataatcg taattatttt tttgtgcatc tctatacaaa ttagacaaca
    12001 ggtattcata tatattcttt tccttatact taaaaaagaa tctttttact tcgctgtttt
    12061 caaaaacttt tgcatttcct atatatcttt tttctaatgg tgttgtagct ctataaagat
    12121 aatttcttcc ttttatgttt ggcataagtt ctgaaactct tcctaatata cttttaatat
    12181 aagtaggtga attgacaact gatttcatag tactatactc tttatatata ttatatcctc
    12241 caaataattc atctgctcct tctccagaca atgctacttt aacatgttta cctgcctctt
    12301 taattaagaa ataaagacct acttctgaag ggtctgctat agggtcatcc aaagaataaa
    12361 ttacattcgg taatgatttt atatattcat cttgtgttat attaatctgg atattttcta
    12421 tacctaactt atcagctgtt ttctttgcta cttctaattc actatatcct ttgacactaa
    12481 agcctgcaga aaaagatttt atatttggat taatttgtga agccacagta gttataatag
    12541 atgaatctat tccaccagac agaaatgttc caatttcttt ctcctcttcc atatgagtta
    12601 ctattgaatc tactactata tttcttatat catcctcgtt cacatctcta ttgcctaagt
    12661 gatttttagt ataaaaaaat tttgtactac ttctaaactc aagtttatta tatctcttta
    12721 aagatagtat accattttct accctaaaat agtgacctgc tggtatcagc cttattcctt
    12781 gtatcatagt atcttcagga agtacatatt gaaatgaaaa ataactttgt aagcttcttt
    12841 catttatatt taaatttttc atatattcaa gtaaaaactt atactctgag gaaaaaatta
    12901 ttgcattttc cttatttata taataaagtg gttttatccc aaataaatct cttgctccaa
    12961 atatactttc attttctcta tcatagatta taaagttgaa cattccttta atcttatgta
    13021 cacaattctt accaaattct atataagatg ctaataagac ttcaatatca tcatttgtag
    13081 taaaattgtg tcctttttta attaattctt cttttatttc atgatagtta tatatatttc
    13141 cattaaaaac aataactttt ccatcttttt caaatggttt atttccataa atagcatcaa
    13201 ttacattaaa tctttcaaaa tcaaaagttt tttcctcatc cttatactct attctactag
    13261 aaagtcttgt attatttaca gattctttta ttttaaattt aatatcttct tttttagata
    13321 tatcaccttt ataataaatt gaaatattgc cgtccatata atcaaaattc ctttctgtcc
    13381 tttagtctta ttctatattt catagttttg tctttggctt taaaacttat tcaatttaaa
    13441 atgaaattgc tattaaattc ttataaatta tctgttaata tttcatattt tttttatttc
    13501 tagaaatact atattaggag gtgattttta tgaagaaaaa aatattaata ccagttatta
    13561 tgtctttatt tataatctca cagtgcataa cttcatttgc ttttacacct gaaaataaca
    13621 aatttaaggt taaaccatta ccttatgcat atgatgcact tgaaccttat atagataaag
    13681 aaacaatgaa actgcatcat gataagcatt atcaagctta tgttgataaa ttaaatgctg
    13741 ctcttgaaaa atatcctgag ctttataatt attctttatg tgaattattg caaaatttag
    13801 attctttacc taaagatatt gctacaactg taagaaataa tgcaggtgga gcttataatc
    13861 ataaattctt ttttgatata atgacaccag aaaaaaccat accttctgaa tctttaaaag
    13921 aagctattga tagagacttt ggttcttttg aaaaatttaa gcaagagttc caaaaatctg
    13981 ctttagatgt ctttggttct ggttgggctt ggcttgtagc tactaaagat gggaaattat
    14041 ctattatgac tactccaaat caggatagcc ctgtaagtaa aaacctaact cctataatag
    14101 gacttgatgt ttgggagcat gcttactatt taaaatatca aaatagaaga aatgaataca
    14161 ttgacaactg gtttaatgta gtaaattgga atggtgcttt agaaaattac aaaaatttaa
    14221 aatctcaaga ttaattaaat attaaaatcc ttttgaaaaa aacttatgtt tgctatataa
    14281 tataataaaa gatagtctaa atttaccaat ataatgttta tagtgacgat tcaggaggat
    14341 ttttatatga taaaagaaac acatgaaagg ggaggttata tattcgcact tttgatactc
    14401 ccctttataa acaatctata cctagttaaa tacgatattg catataaaat agtattaatt
    14461 ataatttata tttattttgc atatttaggt tctttatttc cagatataga tatgagaggt
    14521 tcttacataa gtaaaaaatt tatgttaata tacaaattat ttggaagtag gtttagacat
    14581 agagggttta ctcacagtct aattgcctta ttacttattt atagtttttt taaatctctg
    14641 acaatcttca ctaacaataa tatagttttc tcatgtttgt ccagtgggtt tataatagga
    14701 tatctatctc acttgtgtct tgaccttatt actaaagaag gtattgaatt gttttatccc
    14761 attaccataa acatttcttt actacctata aaaactaact caaaaacaga aaaattcata
    14821 tctaagctat taaatttcat agttattttt ttgattggat atcaatttta tattttatta
    14881 tagttgtaga aaaaataaat aacaatattt tgaactaagt ataaataagt tttatatatt
    14941 ttagtttaca aaagaactaa aaattttgta ctttaataca aaaaataaaa cgtatcttaa
    15001 aataatttag tactaaatca ttttaagata cgttttattt cgtacaagct aaatcatttt
    15061 atcatcttta tcattgtgat gttttttatg atggaaatca taattatgat tttcaagatt
    15121 tttatttact ctatttccaa tcttccatga caaaaacacc aataatataa atgatgctat
    15181 ctttaaagga cttgttataa aaccttctat atcacttgcc acgtaactta taactataaa
    15241 cattacaaat tttcctgcca acattgctgg cacaaaatct tttaaatctt ttttaccaaa
    15301 agctgatgcg atagtaacta aacaaccggg cataaatgga caggcatacg ctataaataa
    15361 taacttaaat ccctgtttat ccatccaact tattgctttt tcagttttac tatttctcaa
    15421 cttattaaaa agcttactat cattaaatct gcttattaat aagaataatg aagttgtccc
    15481 aagtcctgag ccaatccatg ataacaaaca tccaataaat aatccttgta ttgcagcatt
    15541 tgctgtaact atagcaacaa gtggtaaggc tggtataaag ctttctataa atgcacttaa
    15601 taagccgatt attgtggcta acacccaata attttgagtc aaatcaagta gttcttgtat
    15661 atactgcata ttgcttttcc ccttttttaa ctttaactat tattatatac tctatatgtt
    15721 tattatatac caatctcaaa aaatgaatct aatattaaaa agtaaataat ctatataagt
    15781 ttacagttaa ggtataatta ttttattata tattataaca aaaaaggtga tgtttcaaaa
    15841 taaaaatctg aaacatcacc tttttatagc ttaaattgtc atgtaagtta taaaaaatat
    15901 atattagaca taatattact aaaaatgtct agtcagagta aaataaatta cacagtacaa
    15961 caaatattac tatttaattg agttacttct ttataacatt taacctcctt cctactctgc
    16021 tcaataattc atttgatata ctacatgagt tatttgcaac ttcagaagca gataattcag
    16081 ataaattatc tcttcctatt attattgccg tattaccaac ttctacatta ggtatatctg
    16141 taatatcaac agcaagttgg tccatgcaaa ttcgacccac tattgctgct ctacaaccat
    16201 ttattattac gtaacttttt ccacaggata aatttctagg tataccatcg gcatagccaa
    16261 tagataatat agcaattacg ctatctctgc ttgcaataaa gactcttcca tatccaaaac
    16321 tatcatcttt ttgtattttt ctaataagaa ttacttgtga ttttaaggat aaaacaggtc
    16381 gtaaatcaag ttgcaattta gtcgtatcat ttggagaact taatatcccg tataatgcaa
    16441 ttccaattct tacataatta cacttaagat tgggataatt aaaaaaacca taactacttt
    16501 gaatatgtac tttaggaatt tttatacctt tttttaacag aaaatcaatt aacttataaa
    16561 aacattcaat ttgtttattt gtaaagtcta catcttcatc tttcagacta tctgaaacac
    16621 acaagtgtgt aaatatgcca catatatcta agtattttaa tttaaatgcc tttaaaattg
    16681 acctcacatc attaacatca aaacccagtc tgtgcatacc tgtatcaatt ttaatgtgtg
    16741 attttatgtt gtatttctgt ttgtttaaag agatagcatg attaaggtca ataacactct
    16801 gtgtaagatt gtatttatga agttcctttg cccttattgg gttagtatat ccaagaatca
    16861 atatttctcc tagtattcca tatcgacgta aatcaatccc ctcatctatt gttgctactg
    16921 caaatgcttt tacacctatt tggtttgcac aagtcgctac ttcaaaagca ctatgaccat
    16981 aggcattagc ttttacaact gccatcaatt cacaatcttc tggcatagct ttttgtatca
    17041 tctttacatt atgttccaaa ttatttaaat caatttcaat ccaagctcta ttggtattta
    17101 ccttatgttc tcctttcaag tcttttttta gaggtttaag aatcaatata ataattatag
    17161 aagctataaa agatgctata cttaccacta tataatgaat aaggctattg tcaaccaaga
    17221 cagtttgtat tccgaatatt ttagaaaaca tacgaataac aacaatcatc ataggatgta
    17281 taatataaat aattaatgat gatgttcgca ataacctagc tcgagaacct ttcaaaaatg
    17341 ttaaggctgt aaataagaaa tacatacatg gcaataacat aatatacatg ctatcatgac
    17401 gctgtacccc tagtttatgt aaaaacattc cctcacataa cataagtaag aatgatataa
    17461 gaaatccaat caagctattt ttagttgaca tataattaga tttattagat attattccac
    17521 caagtatgaa aaatacaggt gcaaaaaata taccatttcg tgtataatct gatacttcaa
    17581 aaatataccc atataaattc tttaatagtg gtattttttc taaaaaacca taataactat
    17641 caccaaacat accaattaca taaataacaa tagtgattac taatgagtac tttaaaccta
    17701 gtttttttac aagaaagtat gcaatacttg caccaataat tgatgcaggt agataccata
    17761 aatgatataa agtcccatca aatacaatgt ccttaataat attgggcact aaattattca
    17821 tggaaaaata tccagtgtaa atatttaagg gaatataaat tacaatagct atcccgtaaa
    17881 taaataatac attttttaga aaagatttca gtttattaca attataatta tatgttgaaa
    17941 ttagaaaaaa gcctgatagc ataaaaaaga atggcacagc aatacgagca attatactag
    18001 ttaacacaaa gtcagccatt ttactatatg ataataatgg agatgtatga attgcaatta
    18061 taagtaatgc agctataatc ctaaaataat caagccctgc ataagattta ttcctaatca
    18121 ttcttgttcc tccatttctt agtcctttct atttctccaa aatgtcacaa taaaaccatc
    18181 cttattgtta cccgatattt gatatacaca ttgttcaggt atagagattc tcacttcttc
    18241 tttagtatga ataggcacat aaaagatttc tataattttt tctttatctt ctatttttaa
    18301 acaatttgta tacgaaaatc tcttaatata ttctatatat tcttcaagag cgagattcat
    18361 tttattgata atctcagaat gcggatatcc cacataacga aaatgccatg gttcttgaga
    18421 tattcctgtt atttcttctt ttttacactg ataacgctct ataaaaccat aatgtagtgc
    18481 tgccttacga aactcattac aaataccatt ataaggaaat tcagggcaga taaaatctat
    18541 aacttctttc tttaacccta aatcaatagc aagcccagtc tgatgttcac tgtgatttgg
    18601 aagtgctaca aactttttag taaagtcttt tccattgtca cgcagagaac ttgaatatat
    18661 atcttgttgt tcctctgcag tacgatatcc acttacaggt actatttcat ttattccaca
    18721 tatacttttt aaaatatttt gtaaaatgtt tgtagcttca cgtttcataa gaatacttgg
    18781 aaaccttgta tctacaggaa ttaaatctat atcttcagat gttttaactg gtaaaaaagc
    18841 atttactaga attagattcc catcataaac tttatcttta gtcaataaga tagtatttgt
    18901 cattttatct tctttatttt tatctaataa aatattattc atcattttat gtacacctca
    18961 attatcttgt ccaatatttc ttcaaaagac aatccaatac ctttcatcat atttggataa
    19021 cgactatgag aagtaaagcc aggaattgta ttgacttcat taaaaataat ttcaccagat
    19081 ggagttaaaa acatatctac tctagcaaat ccagaacacc caagagttct ataaataatt
    19141 tttgctgtct cttgtattct tttctcacaa tccaaatcaa ttctagctgg catatgaatt
    19201 tttgaacttt tcaatgtata tttttctgta taatcaaaaa atccttttga caattctatt
    19261 tcatctactc ttccaacaag taagtcattg tttcctaaaa ctgcacaccc tacctcaaaa
    19321 ccatcaatat tttcctcaat tattacttca ttatcatgtt caaaagccaa gtctactgct
    19381 acttgtaact cttctttttt ataaatttta ctaataccaa atgatgaacc tgcacgcaca
    19441 ggttttataa ataatggata agttaagtct tttgacagtt cagccacatt aaatttatct
    19501 gagtatttta gtaatattga ttttggtact ttaatacctt ctgaatgaac aagtttatgt
    19561 gatttgtctt tgtccataca taatgctgaa gaaagagtac cacatccaat aacctgtata
    19621 cctgccaatt caaataaccc ctgcactgtt ccatcttctc cattttttcc atgcaataca
    19681 ggaaatacaa aatcaagttt agtgtattta atatcatttt cacatgattc aatagcacta
    19741 tttggaatat gattctgaga aacagcatca ggaattaaaa attcaataat accatttttg
    19801 acacggttct gcgaaacaat aactggaatt atatcagtat ccctttcaaa ccaagtgtta
    19861 ttctggatat tattataatc tccaatataa tgataccaat taccctccct tgtaattcca
    19921 attggtataa tatcatattt ttccgtattc aaattctcaa gtactgcaaa agctgattgt
    19981 aatgatacat catattctgt tgagtttcca ccaaatataa ctgcaatttt tttattattc
    20041 atctttgatt ctcctttgtt gatttattta tttttataca atctacagaa acatgttaaa
    20101 atactttttc tatttcaaat ccaatcaacc ttcacttaca attgcatctg ttccacataa
    20161 tatattttca catttttcta ttgatataat tactgtatca aaataaaagc actcttgttt
    20221 tgatacagta attatatcaa taacaagagt gctctttatt tatttatcct aaagaatttc
    20281 ctattttttt atacttaaat tcttacaaat ttcctacaat cggaataata accttaaagg
    20341 taattatatt gttctcgcta tgagctgtta ttgtcccgtt atgaagctcc actatttctt
    20401 tagctatagc aagtcccaac ccagctctac cactgtttga attacgagat gtatccaacc
    20461 tgtaaaattg ttcaaaaata cgctcaagtt tttcctctgg aatcgtattg ctataattta
    20521 taaatttaag ctttatataa tcatcctcct gtacagctgt tatttcaata gtagtatctt
    20581 caaaactata ataaacagca tttcgtaata agttatcaaa tacacgttgc attttattaa
    20641 catcacacct tatcatcata tttggtgtaa cattcagtgt atattttaaa ttcttactca
    20701 tcaaagtagg tttaaactca tatgttagtt gttcaagcat acgagttaag cttactgtac
    20761 tgtattcaag tgatatattt gataaattaa accttgtaat ttcaaagaat tcattaatta
    20821 aatcttcaag acgctctgcc ttatccaacg aaatagataa atattttttt cgcagttctt
    20881 cagaaatctg actttcatct cgaagtagtg tcagataccc tataactgat gtaagtggtg
    20941 tttttaaatc atgtgcaaga taaacaataa gttcattctt acgttgttct gccatttgtg
    21001 tctcaaattt acgttgttca agtatatgtt taattgagtt tattttcttt tctattgcca
    21061 ataactctgg agataaagcc acttctccaa catcttcttt gataagagag tcaatacctt
    21121 gatttatttc actaaaatat tttgtaaacc aatttaaata aacacggaat attataaaaa
    21181 acacaatcac catagctagt atcatgaaga gctccatgct attacggaaa atctgctgat
    21241 ataaattcaa tgcagcttta tattccatac gaaaagcatt ttcaaagaat gatactatcc
    21301 agatagaaaa cttaccatac aatataaaag aatataaaat ccatatgaaa ataatggcta
    21361 taaatgccat taaagctgtt ctcataaaaa ttttatttct cacttgagta aagtcaggat
    21421 tgactttaat atttttactt ttcaattgta taacctactc cccatacagt ttttataaat
    21481 ttcgggtttt tagaaggttc atgaattttt tcacgtaatc gccctatatg agccataact
    21541 gtgttattat tatcaagata tttttctccc catacagctt caaacaattc ttcagaagga
    21601 accacttttc cttgatgttc acataaatac caaagaatag aaaattcaat cggtgttaat
    21661 gctacttctt tcccaaataa actacattta tgtgtttctt tattgataat caatccttta
    21721 atatcatact cattaacaat tatcgactgt tgttcataag aattattata tcgcatatat
    21781 cttcttaatt gtgtctttac tcttgcaaca acttccaaag ggttaaatgg ctttgttata
    21841 taatcatcag cacctatggt taaacccatt atcttgtcta tatcttcaat tttagctgtt
    21901 aacataatga taggataata gtatttttct cgaattttct ggcaaatatg aaatccatca
    21961 atatcaggaa gcataatatc taaaatagcc atatccaaat ttttagattc aatacattct
    22021 agagcttcct tacctgtata aaacttgtat actgtaaaac cttcattttt taaatatact
    22081 tcaattaaat ctgctatttc tttttcatca tccacaatca aaatattttc gttcatagat
    22141 tatttttcac tctccttatt atcgaggtca tcatttataa tattggtaaa tttacatatc
    22201 aaataattac aagcatattt gtaactaatt tttataagaa aagaactact acatcagaat
    22261 gtgttgatgc taccattgga acacaaaaac tgagattaat tgttattata aaaactcccc
    22321 taacacgtcc ttattatcgc ataacaggta ttaactttca ataaaaatat ctatcagtca
    22381 aacacaattc aatttattat accagagaca atccatataa aaaacaatta aacctatgta
    22441 tgtaaccaca gacattacta aaatttccat gttattttat ataaaaattc aaaaaaggag
    22501 ttgaatttaa aatttgatta gaaaaaaatc aattttaaga caactccttt ttattttaat
    22561 gtatttaaat tatagtatat cagccttcat aatatcctca aaatcacctt tttcagatat
    22621 taatcttatc aatttaacag tacagcttaa atcatttata agtatagctc caactaatga
    22681 accatctttc ataaataatt ttttatatat tctttgacct tcatcttttg atgaaatttc
    22741 tttatcaaaa ttttgtatat taccaatact aaatactttt acattcattg catctaatga
    22801 aactggatat acttcacttt caaatgtcaa attatctcca cttgcatttg cccctgctat
    22861 ttttcccatt tctactgaag atggccatat tgctaaacta atatttccca cttgtgcaac
    22921 atctccacaa gcatatatat ctttaatatt tgtctgtaat gtcttatcta caacaatacc
    22981 tctgtcaatt tttacagatg tatcctgaac catttgagtg tttgctctta caccaattga
    23041 aaatactacc atatcacagt cgacactatc tccatcttta aatacaactt tttgagcttt
    23101 tccatcacct tcaataccat caactgctac acccaatctt acatctattc ctgtatctct
    23161 tacacattgt tctaatattt tactaccttc tttatcaagt tgttttgata gtatgctatc
    23221 catggcttca actactacta cttctaatcc ttttagtcta aattcccatg cagcttcaag
    23281 tcccaataaa ccgccaccaa tcactacaac tttcttagat ttttctagtg ctttttttac
    23341 actatataaa tcttttatat ttctaagagt aaacacattt tctaaatcat ggcccttaat
    23401 tggtggtata aagtttctac ttcctgtagc tattacaagc ttatcaaatt taatagctcc
    23461 atcattcact ataatagttt tattcacttc atctaattta tctacattcg ttcctaatat
    23521 tactactata ttatttttat cataccaatc tttatcttcc atgtagaaat ctgaaccaag
    23581 ttcttcattt ataccatcag aaagtgcagg tctatagtaa gtcaactcat cttcattaga
    23641 aatcatagtt atcttacaag ttttatttct ttttcttata gcatctgctg cataaaaacc
    23701 agctgctcca tttcctacta ttacgaaata ttcatcagta tcttttctaa aagtaacttc
    23761 atcttcttca acttctataa attggtcttc cttagctcca catacagggc atacactagg
    23821 agcactatca ccttcaaaaa cttcattaca aattacacat ttaaatttct tagttttctt
    23881 tttacttcca aaagtagaat ttaacttatc taggaattta cctgtataag aatgtaattt
    23941 atcaatctct tcatttgatg gtttaaagtt aacagcaaaa ggttttactg ctgtcattct
    24001 aagctgactt attctctcca tagcattttc tataccttct ccactccatc catatgaacc
    24061 aaatactgaa gcaacttttc caccatgaag aactgggttt aacgatatta atatatccca
    24121 tataggcttt aacgcatctc ctaatattgt tggtgttcca aataatacac cttgtgatac
    24181 tgctattttt gccaaaatat cttcttgctt atgctctatt acatcaaata aatttacttt
    24241 atagttgcta tttttttcaa tatatgcttt tatagcttct gccataatct ttgtatatcc
    24301 atgtgcagaa acataacaaa ttgtaacttc tttttctaac tttatttgtt catttacact
    24361 ccagttgtaa taaaggtcta taatttttct aggattttca gttaatactg gaccatgacc
    24421 tggacataca gtatctatat ctaaatcttt tatcttttct atagcagtta ccatagatgg
    24481 tttaaatgga cccattatac aatcataata gtatcttaaa gcatccaagt aatccttttc
    24541 ttcattttca tcaagtgtat ttactatctt atcattacta tagtgactac caaatgaatc
    24601 acatgttaca agagttttat cctcttttat atatgtgtat atggtatctg gccaatgaag
    24661 cataggtaca gagaaaaatt ctaaagtttt gtccccaatt gataatgtat ctccatctgt
    24721 aactgcaaca tattcaaagt ctttgtttac gatttcctta agatatttaa ttgctgtttc
    24781 tgaagctact acttttgcat ttttagctaa atccaaaagc ttttcaacgc ttcctgcatg
    24841 gtctggttca gtatgactta caacaatata gtctattttt tcaaaatcta tatttaaatc
    24901 atttaatctt tcaatataat tgtcaaaatg cttatcttta actgtctcaa ataaaactgt
    24961 tttttctgtt ccttttaata catatgaatt ataagtagtc ccataaggtg tatacataat
    25021 tatgtcaaat actcttaaat cagggtcaag tgaacctacc caatataaat tttcttttat
    25081 ttttagtgat ttcaatgaca taccgtactc ctcctaaaat agttatttag tctctttata
    25141 ccctgcttgt tatattttaa acaaaattta ttcaaaaaag aaattatttt aaagatagtt
    25201 taaatcttct aatcttcttt ttctaccttc aatacagttc cctttttaga atctactttt
    25261 atgtcatatt catttttatc tttatctatt cttattttat aaaaagtaac atctaaatct
    25321 ttgtctaaac tccattcttt aaccattcca ttttgttctt ttaatgcaat ttccatagct
    25381 tgttgaggtt taattatgtc atttaaatct aatttttctt ttctagcaac tccatttaaa
    25441 tcttctgaat ccaacttttc tgttttatct tctaatacat cttttgtatt agcatcaatt
    25501 ttcatcttat attcattatt atcatctaca ccttccacag tataaacaaa tttatttaaa
    25561 tctctctcaa gacttaaatc tactatatca gcatccttat atttatcttt aaatatgttt
    25621 attgcatctg tgtatggcac tttaaaatct tgttccatta aattttcatt atttttttca
    25681 tcttttaaag cgttatttga tgcatttgcc tccttttttg tattatcttg tgcttgtcct
    25741 gaatccttag ttggactccc actacatgca gttaataaac ccgctataag catacttacc
    25801 actaataaat acgttttttt acacataaaa accctcctta aatatattaa tttattctat
    25861 attatgccct ctaaaccctt tatttaccct tttatattaa aaattcctcc tattttatca
    25921 attttaaaac tttatattta ctaaaattaa agttacataa aaattatact gtctcaaaat
    25981 ggaaacaagc ctctttttca ttgttgaaat aataaaaata tattttattt cgtacaaaaa
    26041 gagtgcctca tgaactaaaa agttcatttt gagacactcc cattttttta tatttttact
    26101 tacaaaataa cagtacttat taatttttta caaaccttat aagtatatct atatgatatt
    26161 cttaaattta aacactatat taaaaaatta aattttgttt aattctttta ccgttaaatc
    26221 cattttagga tatggaatcg taattccttc ttcgtcaaac ctaactttaa cctcttctag
    26281 taaatcaaga taaattgtcc aataatcttc tgtcttgcac caaactctag ttgcaaattt
    26341 aatagcatta tctccatgtt ctactactcc tataaatggt tctggttcat ttattattaa
    26401 tttatgatta ttaacaatat ctttaagtac tcttctaaca tgtagtatat catcttcata
    26461 ccctacacta aaagtcaaat caactcgtct agtattcttt gctgaatagt ttattagact
    26521 atcatttgat acagaaccat taggtataag tatctgttta ttatctggag taaccaattg
    26581 tgtataaaaa agacctattt gttcaaccgt tcctccatga cctgctgctt ctacgtaatc
    26641 tcctacttta aaaggtctaa tcaatagtat aataaatcct ccagcaaaat ttgatagact
    26701 accttgtaaa gccaaaccta ttgctacccc tgctgatgct atcacagcag caaaactgga
    26761 caaactcatt ccccaatatt ccagaacaat gattataacg gcaactttaa gtacactcaa
    26821 aagaagagac tttaaaaact ttcttaaagt taaatcaata tctcttttac taagtaataa
    26881 cataacatgg tttacaaatt tttttattat tttaaatcca atagaaagta taagtatgcc
    26941 tattataagt tttactccgc ttgtagtagc ccattctaat agactttcca ttatacctcc
    27001 catatccatc ttattaatat ctaaattatt taaatctaac ttgcttatat catctatttt
    27061 cgtcgtatcc ataaaaaact ccttacctta tattttagaa ccaatattat agtttaaagt
    27121 tcttattttc tatttaatat aatacaatac catttgtaca tagtaaacaa gatttatatt
    27181 ccaattatac atattaggaa aatgcaaact atttgttata tatatgcaaa atataatact
    27241 aaattaaact attaataaaa cactatatag ccttaaaatc atcatttttt acccctatat
    27301 gagatgatac tatagttata atacttagta ctataaatac tacaaaacct tttttatatc
    27361 catcaactat attatatgaa tttacttgta aagagatagc tataatcaaa ttaataattc
    27421 cacttcctat aaattcaaaa gtattaataa cactagttgc cagtccagaa taaatttctt
    27481 tatttttata cttggcatca ttaaaaacct gtagatgtga cataactata catcccataa
    27541 taaagaatga tattggtaaa actataattg gtggttttac ctggtatcca actacaatta
    27601 caatccatat aaacaaatct atcattgctc caaattttat tatattaaat ttgctagacc
    27661 ttattttagc aaatacaaaa tccataatta ttgaaccaaa tataaatcca tatgtaaaaa
    27721 atgatactat aaacgcagaa aaactcttac tcacaccata aacgtccatt atgtatctta
    27781 ctccccacaa gcttataaat gcacttgtaa gacccacaaa agagaacatt atcattgaat
    27841 tataccatgt tgatttgttc tttataacag acttaatacc atcaacaata ttaaccttct
    27901 ctgaattttc atagggttca gtatctacat tgaatccata ctcttttggt gtatctctaa
    27961 caataatata cataaaacat ccaattacaa caccaattat accgattaat aaaaatgaat
    28021 ttctccaacc aacaagttca gacaaaaaca caagtggaaa agtagcaaga actccaccta
    28081 aatttcctac aaatgaaaat ttagctgttg cagaagcaaa ttctgactta ttaaaccatc
    28141 tcccttgaat ttttaaaata caaagtaata ttactgatgt tcctgcccct acaattacac
    28201 gagaaatata tgctaattct atactttgaa tcaaaccaaa taaaattgac cctaaacttg
    28261 ccattattat tccaagactg ctaatttttc ttgctccata tttatcaatc aaaattcctg
    28321 caggtatttg cataaatgca tatgcatata ggcaaaagga tgctatattt gaaatttgta
    28381 tagaattaaa acctaactca tttgctaaat tgtctgaaac aacagcagtt gacatcctta
    28441 aaaataatac taaaacaaat gaaaatgtaa ttatacccca aacactccac ttagcctttg
    28501 tataactttt catatcttct ccctcaaatc catatatttt taaattttct tgatataact
    28561 tatataaagt gtatagttat tcttacaaat ttttacacgc aatatcatta tacaacatat
    28621 gtatatattt aactagaaat aaaacactat tcatattata tacatgaatt aactacataa
    28681 ataataagag ccatctttta acattactca aaagatagct ccatataaaa acatttattc
    28741 taaatttaat tttttatcta atataaattt tgtagcgaaa aataacgcta ttgcaactac
    28801 aagactacca agtattgcaa gcataaaact aggactctga taaagatgat ataccatatt
    28861 tgtatcttca tttggtaaga tgcttccaac tgcatctcca acaattgata ttactatatt
    28921 tatagctata aaagaagcaa atgccactat gtttctatgt ttattgaatt ttggcaattg
    28981 tcccattgag attgatgtat ataataacaa aataaatgat gaatatgaaa taaacattac
    29041 tactactaat aataaaatat ctaaaaagtt ttcagaaatc catttaaaac tagtgttaaa
    29101 caaatctaaa atttcaggta gtaaaattcc agaagtacca aaaagcataa gaactccaaa
    29161 agtgaatact gcaactataa aactaagtac agcgtatata agtgctgtta tacatttaga
    29221 taatataagt gaactagtgc taacaggaag agtaaacatt aaataacctt catcccccaa
    29281 aagatttttt ctaaaccttt gaattgtaac tacaatagtt aaaactccta atgccataaa
    29341 aagacttgtt aatactagta ttcctattcc ctgtacctga agtatatttg tattcataaa
    29401 tattccatta aatacagcta ctatcaatat ggctatatat aacggtataa atattcttcc
    29461 acttgctttt aactcatatt ttaataattt acctaacatt taaattcctc cctaaataat
    29521 gcatctatag attttccttt ttcttctctt attgcttcaa cgtctccttg taacactatt
    29581 tctccttttg atatgaatat tatctcatca catatgtttt ctatttcact tattaagtgg
    29641 gtagcaatca gtaaagtact atcttcagaa taatttttta atatagtctt aagtatatat
    29701 gaccttgcag ctgggtcaac accacctata ggttcatcca atatatatat gctcgcattt
    29761 cttgacatta ctaaaataag ttgaactttt tctttagttc ctttagacat tgtctttagc
    29821 ttttcattta catcaatatc taagctttta atcatctcat ttgctcttct aacatcaaaa
    29881 tcactataaa aatcatagaa aaacttaagt aaatctgata ccttcatcca atcatttaaa
    29941 taagttcttt ctggtaaata tgaaactatt tttttagtat ctatggatgg tttcattcca
    30001 tttatcataa tttcaccatt atcaggctgt aataatccat tcattagctt aatcattgta
    30061 cttttgccac ttccattagg tcctaacagc ccgactattt ttccctttgg tatatttaaa
    30121 tcaatatttt ttaaaacatt cttagttcca taactcttgt ttacaccttt aaattcaact
    30181 attccatctt gagaaacatt gcttatattt ttatccattt tataattctc ctttcagatt
    30241 ttctgtaata agttttagca tttcatctct atcatatcca agcttttcta aggttacttt
    30301 taatgtattt atctccacat tagctatttc ctctttcata gcttttattt tttccttatc
    30361 atctgttaca aatcttcctt tagttctctg actataaact aaaccttgtc tttcaagttc
    30421 tgttagagcc ttttgcattg tatttggatt aacttccgca tcttctgcta gtgctctcac
    30481 tgtttctaat ttagatccta ttttaatttc ccctgaaatt atttttaact ttaaatgttc
    30541 tactaattgt atatatatag gtttgttatt atcaagttcc cattccatgt tcatgcctcc
    30601 ttattatatt attgtattaa tttgttaata caataatata ataatacaaa gtatatgtca
    30661 attacttttt agtaaaagta taaaaaattt tacaaaaaaa atgaacacta cattagagtc
    30721 catttttaac acaatttatt aaattcttta tattacagat taattaccct cttaccaagt
    30781 tagatacaat taaatgttca aattcttcaa ttggcattgg ctttccaaac aaatatcctt
    30841 gtgccatatc acactttact tcttttaaaa gtttaaactg ttcttctgtc tcaactcctt
    30901 ctgatatgac tttcataccc aactcttttg ccatttctat tactttagta acaattattt
    30961 tgcttctttt actattataa gtttccataa taaatttttg gtcaagtttt aagatatcca
    31021 taggaatttc ttttaacata tttaatgatg agtaccctga accaaaatca tccatagata
    31081 ttaaaaatcc tatttttttt aaattattca ttatatcaat taaaatatca aaattatcaa
    31141 atatgatact ctccgttaat tcaagttcta tatactttgc aggtatattg tatttatcaa
    31201 ttatattttt gtatgtttct ataaaattat tgcaatataa atgtatcctt gaaacattaa
    31261 ctgaaacagt aactggattt attcctgcat caatccattt tctaaggtat atacatactt
    31321 cttcaagcac atataaatct aaatttataa caaaaccgtt tctttcaaaa aatgggataa
    31381 attcatttgg aggtattagc cctttatcag gacgtttcca cctaacaagt gcttctgaac
    31441 cttgtatctc tcctgtatta agttctatct taggttgtaa gtatataata aattctttat
    31501 tttctagact tgaaaacatt gtattttcca tctctttttc ttttatctct tggtcatgaa
    31561 tcttattatc ataaaatgca tatgaacttt tatgtcctcc ctttgccatt ttatgaggta
    31621 tattagccct atctatgatt gagttgatat ctctatcttc tggaagtata aaatagattc
    31681 cacaacttaa aactaactta tatttttctt cttgattatt attaaacaag tctaattctt
    31741 caaatatagt ttttaacctc tttgttatat cttcttcttc tatatatttc ataattataa
    31801 taaaattatc cgcagatatt cttgcaaaca tttcctcttc aaatatatta ctcttaataa
    31861 tctttgttaa attacgcaaa atttcattcc caacttcata tccaaataaa tcatttatat
    31921 atttaaattt atcaatatct atatataata aagcatactt tacttgagta tttttacata
    31981 gtattttgtt agcatcaatt acaaatttat ctatagtatt tgctcctgta agattatctg
    32041 tatatgctat cctttgcaat ttaattttaa aatttaattt atcatatatt atatagaata
    32101 taagtattac acatattata gccataataa gtattatttg tattgcatgt ttgctaataa
    32161 gctgattatt agacacaata gactcaaagt tatttatgga gttgttttta tagagtttgc
    32221 ataaagaata gactggtgtt gtgtatgtaa tttgtaaaat tatacaaata aataaaaaaa
    32281 acatcaattt tttttgtaaa ttcctcataa aaatttcctc ccattaaaac acctctattt
    32341 tattagcaaa taagtacatc tcaaaagttt ccatttcttg taaaaatgga tagttttgaa
    32401 aagaaaatat taaaattata atataaaaat aaaatttttc agacaattta aacaaaaata
    32461 atcacctttt aataaataat tatatgccaa aataatccaa tttattttct attataccta
    32521 tatgaaatat ataaatcaat agttaagaca atgaatatgc tacattctat aattatagta
    32581 gcagaaaatt ttacaaaatt aaagtagata taaactatcc attttacaag aaatgactac
    32641 tttttattaa aatacagtat ttgcaacata ttcacacttt attttttcat ttttttctta
    32701 ttacaatggt ttcgacaaat cagtaa
//
LOCUS       DACXMZ010000016        31383 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.016, whole genome shotgun sequence.
ACCESSION   DACXMZ010000016 DACXMZ010000000
VERSION     DACXMZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.016"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..821
                     /locus_tag="JD016_02945"
     CDS             <1..821
                     /locus_tag="JD016_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HAU4998588.1"
                     /translation="TIFKYIKLKNLINDLSYDVDDGEIVNLYKNILEEFNITKDIKLK
                     YSYEVETPAFFNSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWF
                     NPFIYIMDKHIDLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLH
                     KQSDIKKRVMNMFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGMEEKDV
                     TNSAIVTMIEFTYAEAPKSMREEHKINCEFLGLIPKDSDKIGVGIEYYDSLLND"
     gene            1063..1338
                     /locus_tag="JD016_02950"
     CDS             1063..1338
                     /locus_tag="JD016_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998589.1"
                     /translation="MYVELHLKWNFKVAIVSSLSILLILIFENNVFKKVSKKFNYCIL
                     MIVVIDILSKKIKYEKITVRTIFSYLIFNIYIIQNFHLFNLEFENHY"
     gene            1737..2759
                     /locus_tag="JD016_02955"
     CDS             1737..2759
                     /locus_tag="JD016_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAU4998590.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANANQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            3055..3975
                     /gene="pyrB"
                     /locus_tag="JD016_02960"
     CDS             3055..3975
                     /gene="pyrB"
                     /locus_tag="JD016_02960"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAU4998591.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            4014..4706
                     /locus_tag="JD016_02965"
     CDS             4014..4706
                     /locus_tag="JD016_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAU4998592.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            4699..5601
                     /locus_tag="JD016_02970"
     CDS             4699..5601
                     /locus_tag="JD016_02970"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAU4998593.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            5686..6270
                     /locus_tag="JD016_02975"
     CDS             5686..6270
                     /locus_tag="JD016_02975"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAU4998594.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            6485..7411
                     /locus_tag="JD016_02980"
     CDS             6485..7411
                     /locus_tag="JD016_02980"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAU4998595.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERN
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(7463..8353)
                     /locus_tag="JD016_02985"
     CDS             complement(7463..8353)
                     /locus_tag="JD016_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAU4998596.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            8541..9572
                     /locus_tag="JD016_02990"
     CDS             8541..9572
                     /locus_tag="JD016_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAU4998597.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            9866..10741
                     /locus_tag="JD016_02995"
     CDS             9866..10741
                     /locus_tag="JD016_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HAU4998598.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            10759..12249
                     /gene="cls"
                     /locus_tag="JD016_03000"
     CDS             10759..12249
                     /gene="cls"
                     /locus_tag="JD016_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAU4998599.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSYCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            12442..12726
                     /locus_tag="JD016_03005"
     CDS             12442..12726
                     /locus_tag="JD016_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAU4998600.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            12759..14387
                     /gene="groL"
                     /locus_tag="JD016_03010"
     CDS             12759..14387
                     /gene="groL"
                     /locus_tag="JD016_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAU4998601.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDVGMPGMGGGMPGMM"
     gene            14649..15389
                     /gene="larB"
                     /locus_tag="JD016_03015"
     CDS             14649..15389
                     /gene="larB"
                     /locus_tag="JD016_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HAU4998602.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            15473..16207
                     /gene="larC"
                     /locus_tag="JD016_03020"
     CDS             15473..16207
                     /gene="larC"
                     /locus_tag="JD016_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HAU4998603.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            16237..16656
                     /locus_tag="JD016_03025"
     CDS             16237..16656
                     /locus_tag="JD016_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HAU4998604.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      16716..16817
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            17038..18573
                     /gene="guaA"
                     /locus_tag="JD016_03030"
     CDS             17038..18573
                     /gene="guaA"
                     /locus_tag="JD016_03030"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAU4998605.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(18650..19696)
                     /locus_tag="JD016_03035"
     CDS             complement(18650..19696)
                     /locus_tag="JD016_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAU4998606.1"
                     /translation="MPAYKDEQRKSWYASFYFTDFDGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRKWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGTIGKKNAD
                     EMNFWTLEEFKKFIEFEKKPEPKLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IKLNGEDIVSSPKTSKSKRVVPIPCFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LNRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(19773..19988)
                     /locus_tag="JD016_03040"
     CDS             complement(19773..19988)
                     /locus_tag="JD016_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU4998607.1"
                     /translation="MLKKLRKKKRMTQLELAEKMRRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKLQEIKRRGK"
     gene            complement(20422..20823)
                     /locus_tag="JD016_03045"
     CDS             complement(20422..20823)
                     /locus_tag="JD016_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002128791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4998608.1"
                     /translation="MQTLAEIIKEYRSEHNLSLREFSNLCGVSHTYIDKIEKNRDPRN
                     GKAVEPTLDMLEKMSFGLNLTLKELLTKLGKIQPNTQQDDELLKKVNSKEDNEVNELI
                     NRLASLDSDDKNAIKKMIDNAYYKAVNTKKE"
     gene            20988..21200
                     /locus_tag="JD016_03050"
     CDS             20988..21200
                     /locus_tag="JD016_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4998609.1"
                     /translation="MKNNLQYIRKESRISQKKFADKIGISRPYLSRIENGKVNPSLEI
                     AHKISLETGKTINEIFFDFTVNHSKL"
     gene            complement(21495..21713)
                     /locus_tag="JD016_03055"
     CDS             complement(21495..21713)
                     /locus_tag="JD016_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4998610.1"
                     /translation="MTKFKDNIKLVRKQMNMTQKQFASLFGISERAYQYYESGSREPN
                     IETLILISNKLNVSTDFLLGLSPNQNRN"
     gene            21826..22020
                     /locus_tag="JD016_03060"
     CDS             21826..22020
                     /locus_tag="JD016_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4998611.1"
                     /translation="MKINNNLKLQREKIGLTQLEVAQKAKITERSYQYYEAGERLPNI
                     RTALKIARILNTNCEKLFNE"
     gene            22062..22874
                     /locus_tag="JD016_03065"
     CDS             22062..22874
                     /locus_tag="JD016_03065"
                     /inference="COORDINATES: protein motif:HMM:PF03374.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAU4998612.1"
                     /translation="MNNLQLNNKNTITTLEIADMLEIRHWEVLRKLEGTEKTKGIIDI
                     LNDNNFVVVDYFIKSTYLDSKNESRPCYNVTKLGCDFLANKFTGEKGIIFTARYVKRF
                     NDMEQELKEQLKCSYMIDDPIERAKCWIKEQEESQSLIKKKDNIIQLQQPKVEKWERF
                     LNNEGLTTIEDFSKTLAIKGYGRNNMYKLLREMEYLKKDNSPYTKYVEQGLFTRKPSG
                     THIEYGKVIEDYKTFLTSKGIDKIMEKLLKLGVIKQSSNVSYVEQKQLSILS"
     gene            22961..23152
                     /locus_tag="JD016_03070"
                     /pseudo
     CDS             22961..23152
                     /locus_tag="JD016_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056356.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23256..23624)
                     /locus_tag="JD016_03075"
     CDS             complement(23256..23624)
                     /locus_tag="JD016_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998613.1"
                     /translation="MKKLSGNYVDIEILSSYCDSMGIKDIIILTNFGFVIGEPDFKND
                     STGIHKDLNKLKVDLVNKMKDELQLVNNTNTIALKNVIVKYPNIQLNFNEMILYPEQI
                     VGFFPVDREDYLEQFSLCQD"
     gene            23705..23902
                     /locus_tag="JD016_03080"
     CDS             23705..23902
                     /locus_tag="JD016_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4998614.1"
                     /translation="MLIGDNISQILRKRNIKPYKLAKELEIDESGLYKIIRNEKKNPT
                     INTLIKIADYLDVTLDELVGR"
     gene            23941..24150
                     /locus_tag="JD016_03085"
     CDS             23941..24150
                     /locus_tag="JD016_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998615.1"
                     /translation="MTKVVAKEQLFYRAKDVAKFLDICEATAYKIIAELNEELEKEGF
                     KTFSGRVSIAYFKERYCYKPRKGVI"
     gene            24252..24479
                     /locus_tag="JD016_03090"
     CDS             24252..24479
                     /locus_tag="JD016_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998616.1"
                     /translation="MKILKVKFPTKILKAGKAIRVTCARFGFESDCIITQSQEFELTV
                     VYFDKELDDLVQSSITIDDAISYDYCIEMLN"
     gene            24492..24809
                     /locus_tag="JD016_03095"
     CDS             24492..24809
                     /locus_tag="JD016_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998617.1"
                     /translation="MKSLIIVRNAVEQQLNRANLEINKNEELYTKLRKKEERNILEEI
                     ELSNALREKSVNERLKIFAESLLEIIDTQIEIKEYEESEDYKIFQLILEELERDRPID
                     VQI"
     gene            24894..25403
                     /locus_tag="JD016_03100"
     CDS             24894..25403
                     /locus_tag="JD016_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HAU4998618.1"
                     /translation="MSTLYELTTDLLEIEEGLTEITGNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVIIDIDSDINSLDLEIKRMQELKKIKKNSLDRLKSNIKECMELLGIKKVETFL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNTIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            25413..26021
                     /locus_tag="JD016_03105"
     CDS             25413..26021
                     /locus_tag="JD016_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAU4998619.1"
                     /translation="METNNVYIKLVNIQSTLKAPKSQFNSFGKYNYRSCEDILEGLKP
                     ILKEEKALVVLDDNIVQIGNRFYVEATATLIDAETGEKVSTKALAREDETKKGMDLAQ
                     VTGSVSSYARKYALNGLFCIDDTKDSDATNKHGNEQKKKEVNESELNILYSLGESIEK
                     DKNRVDSEVYKKFGKLAVDLTKQEYEKVLNGYKSILEKQKQE"
     gene            26031..26912
                     /locus_tag="JD016_03110"
     CDS             26031..26912
                     /locus_tag="JD016_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAU4998620.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDVKLSWQAKGLHSYLISKPDH
                     WKIYVNDLYKRSKNGRDATANILRELIENGYITRTPCRDSNTNKMLGGYDYQVYEIPL
                     ENPQKLKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQPQQDETT
                     YIKKYFEKYIGVITPNNFIELMSYLDDGMEADVIIRAIDEAISNGVKNYKYVKTILNN
                     WIEAGVKTNLELTEYQNEFERKKKSKQEKKQSNHKNVNTPNVSKNKFHNFNETFTQYS
                     PDELDDIIKKSQKVKFK"
     gene            26972..27145
                     /locus_tag="JD016_03115"
     CDS             26972..27145
                     /locus_tag="JD016_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAU4998621.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKVAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            27161..27577
                     /locus_tag="JD016_03120"
     CDS             27161..27577
                     /locus_tag="JD016_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAU4998622.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKGKPIYAVGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKSDSNNHKEQQEYIPPGLDPQGFQAIDDDDIPF"
     gene            27651..27923
                     /locus_tag="JD016_03125"
     CDS             27651..27923
                     /locus_tag="JD016_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998623.1"
                     /translation="MKYECEKVFLECDKGSFEINDTRTEEVTFEGTEIDNPFKRVKYE
                     GKATFEIVSGWEYLQREMLWFKILHLSAVVAKIMQYKMLGILIREM"
     gene            27928..28308
                     /locus_tag="JD016_03130"
     CDS             27928..28308
                     /locus_tag="JD016_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998624.1"
                     /translation="MAKIWMDANEVLSKTMDLEDMFELNLRAIRKRNEKIKNEIEKQI
                     KYEDSEIKTTNRGGPGKIIKIFNINTGEVKILKSAEEASRYIKVSRSYASYLARENKS
                     TEDGWKAEYIQEVSDGISKCGTSN"
     gene            28292..28426
                     /locus_tag="JD016_03135"
     CDS             28292..28426
                     /locus_tag="JD016_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998625.1"
                     /translation="MEQVIKLAEKILNKKKCSVNKAIDIAIKILSKYECEGMIRNEFN
                     "
     gene            28413..28862
                     /locus_tag="JD016_03140"
     CDS             28413..28862
                     /locus_tag="JD016_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998626.1"
                     /translation="MSLIKYRGYDFENEKWIYSATIMWSDAVDCLFMLKENCEWQRVC
                     NVGVCSGEWARNNQEICEGDILKEYDNFHDTSQYGIVKRSFNSIKIYLEWHYLKKLEG
                     EWIELINKTEIYHSRDYKIVGNEYENLEEVRTEFLERKEMLENEHLG"
     gene            28846..28962
                     /locus_tag="JD016_03145"
     CDS             28846..28962
                     /locus_tag="JD016_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998627.1"
                     /translation="MNILASVLLIGISFIAGRVYEYRLNLKECENCDNKRGV"
     gene            29251..30324
                     /gene="dcm"
                     /locus_tag="JD016_03150"
     CDS             29251..30324
                     /gene="dcm"
                     /locus_tag="JD016_03150"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAU4998628.1"
                     /translation="MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHK
                     PKEDEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
                     RERIFIVGHFRGRSTRKVFPIERKSGKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGG
                     GGAKTGLYFIDLNKNSKVTINARCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQ
                     NGRRIKESGEPMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDS
                     QLYKQAGNAVTANVVYEIAKRMG"
     gene            30408..31103
                     /locus_tag="JD016_03155"
     CDS             30408..31103
                     /locus_tag="JD016_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998629.1"
                     /translation="MDLNKIMNDALVEIEESGFVEETVKKELEETIKRAVNEIFGSYS
                     KFRKNIEQHLGENININLDELDIQKYNLLVANVVKEKVDATMKGQGIEHLKRNLDNML
                     VGLKKEYKMSELLEELKEDKYNLDEYPCDEDCITFILESNFNSEWARWIDFDECPNVS
                     RNNCEHSILLRNDGTIAAFRYQNREISSKDIMNGFGNFGDLLFKIYAHNSKIILDLGN
                     DVDDYDLTNGEDY"
     gene            31119..31340
                     /locus_tag="JD016_03160"
     CDS             31119..31340
                     /locus_tag="JD016_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998630.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSIENLKKKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMAEYYCDGGIEI"
BASE COUNT        12658 a         3222 c         5719 g         9784 t
ORIGIN      
        1 acactatatt taaatatata aaacttaaaa atttaataaa tgacttatct tatgatgttg
       61 atgatggaga gattgtaaat ctttataaaa atatattaga agaatttaat ataacaaaag
      121 atataaaact caaatactct tatgaagttg aaacaccagc attttttaat tcatgtgtgc
      181 ttttgccacc acatgagtat aaattgaaag agttagactg gatttttaga catgagctta
      241 tgcattttaa gagtagggac ctttatttaa agtatgtaat cctgttttta aaaacagtat
      301 attggtttaa tccatttata tatataatgg ataaacatat agatttagat tgtgaacttt
      361 attgtgatga aagagtttta aaaaatcgaa attcagagga aaaaaaggat tatgctttaa
      421 caattctaaa tgcaatgaga aaaggttcaa atacttcaaa taaatttata gctggacttc
      481 ataagcaatc tgatattaaa aagagagtta tgaacatgtt taatgaaaag tacaagaatg
      541 gtatattaat ggcattaact ttatgtttat tatcttctat tacttttttg aaggttaatt
      601 caactagtat tgttaatctt aatgtattgc caaaaggaat ggaagaaaaa gatgttacta
      661 atagtgcaat tgttacaatg atagaattta catatgcaga agctcctaaa agtatgagag
      721 aagaacataa aataaattgt gaattcttag gtttaatacc taaagactct gataaaatag
      781 gtgttggtat agaatattat gacagtctat tgaatgatta aataaatatt attttaacag
      841 atattaatta taaagttttg aatagtaatt tgttgagaaa taaatatatc cttataaaaa
      901 tagatactta tataccaaga ataaaaaata gttaagatag tacacaagga tatacttata
      961 aagaacttaa ataagtgaaa taaattatta attataaggt ttataatttt aaaaaattag
     1021 ataaattcag gtgtaaaata ctataaaaca caattcggaa aagtgtatgt tgaacttcat
     1081 ttaaaatgga atttcaaagt tgctatagta agtagtttaa gcattcttct tatattaatc
     1141 tttgagaata atgtatttaa aaaagttagt aaaaaattta actattgtat attaatgata
     1201 gttgttattg atatattatc taaaaaaatt aagtatgaaa agattactgt taggacaatt
     1261 ttttcatact taatttttaa tatttatatt atccaaaatt ttcatctttt taacttagaa
     1321 tttgagaatc actattagga aataggcttt gatttatgta gggttaaaaa tttcacagaa
     1381 tatctaactt tatactaaat ccatcaaaaa atatacgcca tataaaagcg cataaaaaaa
     1441 cacataaaac aatatttatg cgcactttaa cctcgaatgg tacaaaattc tttactttta
     1501 cagacccata ttatataatt aagtaaataa tgtatattta ggtgacatat tggataagct
     1561 aatttattta ttttattaat ataataatac tataaaagtt atataaagtg gtttacaaaa
     1621 aagttacaga tagagctttt attaatgtat tgtgtattag tattttatcc ttaggtacac
     1681 ttcacctaca aaatagaatc ataagataat tatgggaaaa ttttaggagg tttatcgtga
     1741 agaaaaagat attaatacca gtatttgcat cagttatggc attatcagta agttcaattg
     1801 taaatgcaga tgaagtaaat gattcatctc aaaataagga tgataaaaca aatactgaat
     1861 taaatttagg tgaatataaa gaagtaaaat ataaagttgc aaaaataaaa gatggagtag
     1921 ctataaagat aagagaagaa ggtcaagttc aaaacatagc ttattctgga gatgaattta
     1981 cggttttagg aactcaagga gaatgggtta aggttaaagt tgaagatggc gaaggttggt
     2041 tagctactag atatgtagat atatctgaag gtgtaggata tactaacgct gataaagtaa
     2101 acttaagaaa agataagagt gaaagttcag aagtaattga agagctggaa aaaggaagtt
     2161 ctttattggt tttggaagat aatggagatt ggttaaaagt taaagatggg gaaacagaag
     2221 gatatgtaaa atcatcttat atatctgata aagctcctgt aatagaagag ccacaagtaa
     2281 acccaagtat tgaccaaaat gtagattcta caaataataa tcaacaaaac aatgcaaatg
     2341 ctaatcaaca aaataataat tcaaatgtgc caacagctaa ctctaatgca gttcaagctg
     2401 tacttaactt ggcatattct aagcaaggtt gtccatatgt ttggggagca gaaggaccaa
     2461 acacttttga ctgttctgga tttacacaat atgtgtatag aaatgcagta ggaaagaata
     2521 taccaagaac atctaaagca caatctaaat atggacaaac tgtaagtaaa gcaaatttac
     2581 aaccaggaga cttagtattc ttcacaacta atggttctgg ttctgttagt catgttggaa
     2641 tatatgtagg tgggggtaat atgatacatt caccatcaac aggaaaaaca gttagtgtaa
     2701 ctagtataaa ttcatcatac tatacagcaa gatttgttac agcaaaaaga atattataat
     2761 cggtttgtaa tatgacaaat tgataaatac ataaatatat ataaacgctc tatattatat
     2821 ttaatttaat catggaaaca aaaaatgatt atattttaaa tgtactatag agcgtttttg
     2881 tatcacttta aatctaatca agattcaagt taatatgact gttaaatagg agccttttca
     2941 tatgatgatt atttgcgcaa tcccttgaca ggatagggca aaggtggtaa gctactaaca
     3001 gtacaaaaat acttttaatt ttagttcaga gaggctaaaa aaggaggacg aaagatgtta
     3061 aaatcaagaa atttaatcca accagaagac ttttcaatag aagaaataga tgaaatatta
     3121 gaattagctc aaaaaataat tgacaatccg tcaaaatatt ctcgtatatg tgaaggaaaa
     3181 ctattagcta cattatttta tgaaccatca acaagaacaa gattaagctt tgaatctgct
     3241 atgaacagat taggaggacg tgtagtaggc ttttcagagc caaattcatc ctctgcatca
     3301 aaaggtgaaa ctctaggaga tacaatgagg atagtatctg gttatgttga tataatagct
     3361 atgagacatc ctcaatcagg tgcagcttca gaagcagcta gatatacgga agtgccattt
     3421 ataaatgcag gagacggcaa aaaccagcat ccaactcaaa cacttacaga cttacttact
     3481 ataaaatcat taaaaggaac tttagaaagc catacaatag gattatgtgg tgatttaaaa
     3541 tatggaagaa ctgtgcattc tttggttaag gctatggcaa gatataaaaa tacaaagttt
     3601 gtatttatag cgcctgaaga gttaaaaatg ccagattata taaaagaagc tataaaggga
     3661 catgcatatt atgaaactaa taatttggat gatgttatag gaagcttgga tgttttatat
     3721 atgactagag ttcaacaaga aagatttgaa gataaatcag agtatgaaag acttaaaaac
     3781 tattatattt taaataaagc taaattagaa aaggcatcta aagatatgtt agtaatgcat
     3841 ccattaccaa gagtaaatga aatagacata gatgtagata gtgatgatag agctgtatat
     3901 tttaaacaag ctaaatatgg aatgtatgtt agaatggctt taataataaa acttttaggt
     3961 ataaatgaag actaatagtt atcatataat agtaaaacaa aggagatttt gggatgtata
     4021 aaattctgga aaatatatat ataggcgaag atatgtatag aatgaaagta aaaggtaatt
     4081 ttgaaggtaa aatgggacaa ttctatatgt taagagcatg ggatacttat ccagtacttt
     4141 caagacctat aagtatacat gatatagatg aagaagggat aacttttctt tataaagtag
     4201 taggagaagg aactcaaatt ttgagtaacc taaaggtaaa tgatactata aaattggaag
     4261 gcccttatgg taatggatat gctaaggttg atggaaaggt agctctagta ggaggaggaa
     4321 tcggtgtagc tccactatat ttggttgcta aaaatataaa aaattgtgat gcatatcttg
     4381 ggtttagaga agatgttata ttagaagatg aatataaaca agtatgtaat aaagtctata
     4441 caacagtagg aaatactttt gtaacagata taattgatgt tgagaaatat gattatatat
     4501 taacatgtgg tccaactcca atgatggaaa aattagttaa gatggtagaa ggtactaaaa
     4561 cgagaattat ggtatctctt gaaaaccata tggcttgtgg agtaggtgcc tgtttagtat
     4621 gtacgtgtaa aactaatgga ggaaataaaa agacttgtaa agatggtcca gtgttctggg
     4681 gagaggacgt gatttttaat gggtaattta agtgtgaaat ttggaagtgt agaatttaaa
     4741 aatccagtaa taatggcgtc tggaactttt ggatttggaa aagaatataa tgagatttat
     4801 gatatacaaa aacttggtgg tataagtagc aagggtctta ctttaaataa aaaacctggt
     4861 aacaatggta tgagagttca tgagacacca tccggtatga tgaatagtgt tgggcttgaa
     4921 aaccctggag ttcaaggatt tatagactat gaattacctt tttttagtaa actagactta
     4981 gtcagaatag ctaatgttgg aggtgggaca ttagaagact acttattagg ggttcaaatg
     5041 ttaaatgaca agcctataga cattattgaa ttaaatatat cttgcccaaa tgtcaaagct
     5101 ggtggaatgg cttttggtat aaaaaatgaa gtggcaagag aagttgttcg tgaggtaaga
     5161 aacataacga aactgccttt agtaataaaa ttatcaccca atgcagaaga tattgtagga
     5221 atggcaaaag tttgtgaaga agaaggtgct gatggagttt cattagttaa tacttttaaa
     5281 gcaatggcaa ttgatataaa aaatagaaga ccagtatttg aaaatgtata tgctgggtta
     5341 tctggaccag ctataaaacc tatagctttg agaatggtac atgaagtgtg taaaaatgta
     5401 aatatacctg taatggggat gggcggaata actaaagcca cagatgctat agagtttata
     5461 atggcaggag ctacttgtat ccaagttgga actgcaaatt ttatgaatcc tagaataggt
     5521 attgaaataa tagatggaat taatgaattt atggatagag aaggaataaa aagtttagac
     5581 gaaataagag gaattatata aggtagaata aatagtattt taaagataaa aaatatagat
     5641 taaataaaca ataattttat aacattaaca tggaggaaaa ataatatgag taaagtagat
     5701 gtagtggata tattaaagaa aagtgatgca ttgttggagg gacatttttt attatcttca
     5761 ggaaaacaca gtaatagata cgtacaatgt gcaaaagtat taagattccc agaatatgct
     5821 gctgaggtat taagtacagt tgttgaacaa ataaaagact tagatataga cttagtagta
     5881 ggaccagcta tgggtggagt aatagtttct tatgagttag gaagacaatt aggaaaagaa
     5941 gctgtattta ctgagagaaa agacaataca atggagttaa gaagaggatt tgaagttaaa
     6001 aaaggagcaa agataataat tgctgaagac gttgtaacta ctggtaaatc aactatggag
     6061 acaaaaagag tattagaagc cttaggtgga gaagttgtag gtgttgcatg tatagcagat
     6121 agaactaatc atgatatagg tatgcctata tatagtgcta taaaacttga tattcaagtt
     6181 tatgaatctg atgagtgtcc tttatgtaag gaaggaaaat taccagttgt taagcctgga
     6241 agtagagagt tcaaggaatt agggatgtaa taaaaactta attatttata gtgttactta
     6301 aaaaatgtaa attttttaga gtaaaaattt gtaatataac atataactta aaaataaaaa
     6361 gcagttagcc ttataaatga aggttagctg ttttttttgt taaaaatata tttacaaaat
     6421 aagagatagt agcagtacaa tataagtata aggtaaaagt attatatatt agggaggggt
     6481 aagtatgaaa aatcaattaa atgaaaatca taaaaaggtt gtattttttg gagtaggtgc
     6541 tgtaggagct acttttgcag aacaattttt taactctaaa tatgatttta aaattctttg
     6601 tgataatgaa agaaataaaa gatatttaga agaaggattt ataataaatg gaaaaaggta
     6661 tgattttgat tatgtaacta aagatgagta taaacaagag gctgatttta taattatagg
     6721 tctaaaatat aataatttaa aagaaaatat aaaagaatta gatggattag ttggaaaaaa
     6781 tacagttata atgtctctgc taaatggagt tgatagcgaa gagataatag gagaaagatt
     6841 tggaattgaa aaaatggtat attcatatgt taccaatata gatgcaaaga aaatcaataa
     6901 taatattata cacactacta atgggataat tgtatttggt aacaaagata atagtgaaga
     6961 tagaaaaact aatataataa ccgaagtttt tgatgatgta aatatagaat atactttatc
     7021 aaaagatatt caacgagata tgtggtggaa gtacatggtt aatattggtg taaatcaaac
     7081 ttcagctata cttggtgcac cttatggagt ttttcagagt tctgagcatt taagagaatt
     7141 agcaaaatct gcaatgaggg aagttgttgc tatagcacaa gcaaaagaca tatctcttac
     7201 agaagatgat gtggaacatt cattacatag aatactagaa cattcaaaag aaggaagaac
     7261 atcaatgctt caagatgtgg aagctcatag acttacagaa gtagatatgt tttctaagaa
     7321 tatctgcaaa cttggaaaaa aatataacat acctactcct ataaatcaga ctttctttta
     7381 tatgataaaa gtaattgaaa gtagatttta aagggtcttt gtaagaaata ttgataatta
     7441 ttgattttaa attttattaa tgttatatac tatttaagtt actaaatatt tttaactctt
     7501 caatgaaaat agttgctgct tttgataaag ttatatcttt tggaaatata tatgagaaat
     7561 gtctgttgta gctgcttcca agctctatga cagacagctc attgtttaga acaggtaagc
     7621 ttgtcactag attagagact attgtaattc ctaaattatt tctaacagat tctttgacag
     7681 cataattact tccaaaaacc atcatacttt ttggaataat ttctttaaca cttaaaaaca
     7741 tatctaaata atctctggtt ccagaaccat cctccctgac tatccatttt tggttttgaa
     7801 gtttatcaaa actaaaatct tttaataaat gactcttgta tggaagtgct aaaaccatct
     7861 tatcttcaaa aaaatattct tgaataaatg aaggggaaga gcaagtgcct tcaataagac
     7921 caatgtctaa aatataatcc ttaacatgag aagatacgat tgatgtattt tttataaaaa
     7981 cttctacatc tatgtcagga tactttttag aaaataatgc tagaaaattt ggtaaaatat
     8041 actctccaat agttaaactt gcaccaatct ttaagtgacc agttatagca tctgaaactt
     8101 ggcttacatc atgataagta atatttaaaa gatttaatat ttctttagct cttttgtaca
     8161 agatttgtcc actttctgtt ataaaaatgg ttttctgttt aatagacctg tttataagca
     8221 caactttaaa ataattttct agatttttta tgtgtttact tacacttggt tgtgatagat
     8281 ttaaatattc cccagcttta gtaaagtttt tgtattcaac aacagctaca aatgttttta
     8341 attcttcaaa caattttttg tctcctttac ctaatgaaat tttacatttt ttatatactt
     8401 tctacagata attatactat atataataac cattaaaaac attaatcatt acgatattat
     8461 atatttattt tacttatggt tattacaatt atatactatt gctatgaaga aaactatatt
     8521 aattataagg taggtatatt atgttaaaga aaaaaagcag tttgacatta aaaactataa
     8581 gggaaatatt accaggactg tttgtatcag tattagtagg ttatattagt atatttattt
     8641 ctattttaat tcctaaggta ggggcagcat caatttctat atttttaggt atgtttgtag
     8701 gaaacctatt tttaaatcaa aaggtatttc aaaaaggtta taaattttca gagacagatt
     8761 tgttatcata ttcaatagta ctattaggag caacactcag tgtatctacc ttaatagact
     8821 taaaagtttc aggaatttta tttataatat tgcaaatgac aataacaata atagctgcac
     8881 tttatatagg taaaaaacta ggatttgaag aaaactttag gtttctaatg gcaagtggaa
     8941 atgcagtatg tggttcttct gcaataggag caacagttcc agtggttaat gcgacagata
     9001 aagaaaaggg aattgcagta actattgtaa atgttactgg tatatttctt atgtttttac
     9061 ttccaataat atctcaattt ttatatagcc atgaacttgt aaaaacttca gcaatgatag
     9121 gtggaacttt gcaatcaatt ggtcaggttg tagcaagtgg agcaattgtt ggagaaaatg
     9181 taaaagattt agctactatt tttaagatag taagagtaat atttctagtt gttgttgtat
     9241 tagtatttgg acatatgaaa aatcaatcta atagggaaat tcttgaagaa gaaaaggatg
     9301 agataaaaaa gaaaaaagtg actataccat ggtatgtaat tggattcttt ataacttgtg
     9361 cacttttttc aatgaacatt ataccacaag aagtatctgt gttgtgcaaa gaaataagta
     9421 acaaactaga aataatagct ttagcagcaa ttggattaaa ggttaatgta aaagatttag
     9481 tgaaacaagg gaaagaagtt tctttatatg gtttatttgt aggtatagta caagttgtat
     9541 cagctgtagt tttaataaaa atatttatat agcataaaaa tagtatttgt aatattaatt
     9601 tgattaatta aaataatatg atactaataa tttataatat aaataaaaaa tgaatttcga
     9661 taagtttaag tgggtataaa cttatagaaa ttcatttttt atttaaggta aaaatttaaa
     9721 gtaaaatatt attttacata ataataacct tataaatttt gaaattactt atctttgtgt
     9781 taaaataagt aaaatgcaca tattagagct aagtttttaa gtaaataaac aaaatgaaaa
     9841 tataaatatt aaggagattt ggtatatgaa tgtttatgaa aaaggtaatg gagttatact
     9901 agaaggagta acagattttg acccagtaca tatatttgag tgtggtcaat gttttagatg
     9961 gcacaaacaa gaagatggct catacactgg agttgcaaag gggagaatat taaatgtcaa
    10021 aaaagagaat gataaaatat atttaaataa tactaattta aaggagttta acagcatttg
    10081 gtataattac tttgatttag gaactgatta tacagaaata aagaataagc taaaaaatat
    10141 ggatgaatat ttaaataagg ctactgaatt tggatgggga ataagaatat taagacaaga
    10201 tggatgggaa atgcttatat catttataat ttcctctaat aataggattc ctatgataca
    10261 aagagctata gaaaatttat caagaaagtt tggtaagtat ataggtgaat atgaaggtaa
    10321 tgaatactat gcattcccaa caccagaaga actaaataag gcctcacaag aggaaataag
    10381 agcatgtcaa acaggtttta gagataagta cataaaaagt actacacagg cagttataga
    10441 gaataatgat gaggtatctg aatatacaaa tttaagtaca gaggattgta gaaaagagct
    10501 tttaaaattt aatggagtag gaccaaaagt atgtgattgt attgcattat ttggtatgca
    10561 aaaatatgat tcttttccag ttgatgtttg ggttaagaga gttatgcaag aattttatat
    10621 agatgaagac atgagtttac ctaaaatgag aacttatgga atcgataaat ttaaagaaat
    10681 gtctggattt gctcaacagt acttatttta ctatgcaaga gaactcggta taggaaaata
    10741 aattaagggg gagaaattat gggagtaata ggaacaatat ttctgttcta tttaattata
    10801 tcatatctag ctggagccat aatttctgtg attatattac ttgaaaatag agaccctgca
    10861 aaaacaatgt cttggctatt gatgtttatt atatttccag gtgtaggttt aatgatatat
    10921 gctatttcag gtagaaatat tagaaaaaga aaattattta agactcaaaa actagctaat
    10981 aatataaaag aaaagaaact atttgataca ttagaaaaga taacagaaat agttgaatta
    11041 gaaaaagaat ctataaaaca aaataagctt ttaagagatg aagaggatgg tagttataga
    11101 aaaagggtaa taaatatgtt gttaaagact ggtatgtttc cctttactaa aaataataaa
    11161 gtggatgtat ttgtagatgg aaatgaaaag tttaaaagac ttatagaaga tataagagaa
    11221 gctaaagacc atattcattt agagtacttt atcatcaaag atagtgaaat aggtagggtg
    11281 ttaaaagaag aacttataaa aaaggctaaa gaaggcatta agataagaat tttatatgat
    11341 gatgtaggat gctggagatt ttggtttaat agaaaatttt tccgagaaat gagggaagta
    11401 ggtatagaaa ttgcagcatt tttacctaca aaatttccta taataggagg gaaattaaac
    11461 tatagaaatc acagaaaaat tgttgttatt gatggaataa ttggatatac aggtggaata
    11521 aatataggag atgaatattt aggcaaaaat gataagtttg gatattggag agatactcat
    11581 attaggatta agggtatttc tgtatacatg cttcaaatga catttctgat tgattggtat
    11641 tatacgacaa aagaagtatt agtgactaaa aattatttcc caagtgtagg gaatgttggt
    11701 gaaagtatga tacaagttgt tgcgagtggt ccagacagtg actgggaaga tattcattat
    11761 gcttattttt ctgcaatatg tcaggctaga aaaaatgttt atatagaaac tccatatttt
    11821 attccagatg aaagtctact aaaagctata aagagtgctg ctcttagtgg agttgatgtg
    11881 agaataatat ttcctaaaat tgcagaccac aagatagtaa atatagcatc ttattcatat
    11941 tttgaagaaa tattaagagc aggtggtaaa gtttatttat ataataaagg atttatacat
    12001 tctaaagtcg taataataga tgataaaatt gcttctgcag gtacagctaa tatggattta
    12061 agaagtttta tgcttaactt tgaggtaaat gcatttatct atgatgaaga agttattagg
    12121 gtaatgacag atgatttctt tgaggattta agttactgtg aagaacttaa tttggaggta
    12181 tttaagaata gaaatatcat acaaaaaatc aaagaatctg tagcaagatt attttcacca
    12241 atattataaa aaattattaa aaaaggtatt gaaaattatg tgtgattggt atattataat
    12301 aattgttagc actctattag atagagtgat aataaataaa aagtatatat ataaatctac
    12361 atatataagt agtattaatt taaaaataac taaaaacata ggcgaactag tctatactta
    12421 atattttagg aggcgtgaat aatgaaaata agaccattag ctgacagagt agtaattaaa
    12481 aaagtagaag cagaagaaaa aactgcaagt ggaatagttt taccaggagc agctaaagag
    12541 caacctcaaa tagctgaagt tgtagaagtt ggaccaggtg gaatagttga agggaaagaa
    12601 ataaaaatgg aattgacagt gggagataag gttatattcc aaaaatattc tggaacagaa
    12661 gttaagatag aaggacaaga atacacaata ctaagacaga gtgatgtatt agctgttatt
    12721 gaataaatat agaataaatt tattaggagg ggtttaaaat ggctaaagaa attaaatttt
    12781 cagaggaaac aagaagagct ttagaagctg gtgtaaataa attagcagat acagtaaaag
    12841 taacattagg acctaaagga agaaatgtta tattagataa aaaatttggt tctccactta
    12901 taactaatga tggagtaact atagcaaaag agatagaact agaagataga tttgaaaata
    12961 tgggtgctca attagttaaa gaagtagcaa ctaaaactaa tgatgtagct ggagatggta
    13021 ctacaactgc tacagtttta gcacaagcta taataagaga aggtttaaag aatgtaacag
    13081 caggggctaa cccaatactt ttaagaaaag gaatacaaaa agcagtaaca gtagcagtag
    13141 aagaattaaa aaatcaatca agaatagtag aaacacaaga ggctatatct caagttgctt
    13201 ctatatctgc tggtgatgaa gaagttggaa aattaatagc agaagctatg gaaatagtag
    13261 gtaaagatgg agttataact gttgaagagt ctcaaactat gaatactgaa ttagatgctg
    13321 ttgaaggtat gcagtttgat agaggatttg tttctgcata tatggttaca gatgtagata
    13381 aaatggaagc agttttaaat gacccatata tattaattac tgataaaaaa atatctaaca
    13441 tacaagaatt attaccagtt cttgaacaaa tagttcaaca aggtaaaaag ttattgataa
    13501 tagctgaaga tgtagaaggt gaagcattat ctacattagt agtaaataaa ttaagaggaa
    13561 catttgatgt agtagcagtt aaagctccag gctttggaga tagaagaaaa gaaatgcttc
    13621 aagatatagc aatacttaca ggtgctcaag taatatcaga agaattaggt tatgatttaa
    13681 aagaagctga tttatctatg ctaggtagag cttcatctgt taaggtaact aaagaaagta
    13741 ctacaatagt agatggttct ggagataaaa aagctataga agatagagta actcaaatta
    13801 aacatcaagt agagcaaact acttcagatt ttgatagaga gaaattaatg gaaagattag
    13861 ctaaacttgc tggaggagta gctgttgtaa aagttggagc tgctacagaa gtagagttaa
    13921 aagaaagaaa gttaagaata gaagatgctc ttaatgcaac tagagcagct gtagaagaag
    13981 gaatagtagc tggtggagga actgcttttg ttagtgttat accagcaata ggaacactaa
    14041 ttgagagttt agaaggagaa gttaaactag gtgctcaaat agttaaaaaa gcgttagaag
    14101 agccattaag acaaatagct ataaatgctg gtcttgaagg tgctgtaatt gtgcaaaatg
    14161 ttgttaattc tgaagcagaa actggatttg acgcattgaa tgaaaaatat gtaaatatga
    14221 tagaagctgg tatagttgac ccaactaaag ttagtagaag tgctttacag aatgctgcat
    14281 caatagcaag cactttctta actactgaag ctgctgttgc tgaccttcct gaaaaagaag
    14341 atgtaggaat gccaggaatg ggtggcggaa tgcctggtat gatgtaattt aaaaaattta
    14401 aaatataaat tttaatataa agaactattt cgacttagat tttaaactct aagttgaaat
    14461 agttcttttg tatatatcta atttttgtat atctataaat tcaatacaca tatttagata
    14521 atcagtatct agtttgtaat aatattttag agtagtattg ttatttttta ggttatgatt
    14581 ttagatatat attaattaag gtataattgt tgatagagaa taaatcaaat ttaaaaggat
    14641 gcgataatat ggacttaaga aaattgcttg aagaagttaa aaatgaaaat attgatatag
    14701 attttgcgtt agaaaagtta aaagatttac cttatgaaga ccttggatat gcaaatatag
    14761 accatcatag agaattaaga aatgggtatc cggaagttat ttattgtgaa ggtaagagtg
    14821 atgagcatat tttagggatt attgataaaa tgaatcaaaa aagttctaac atattgggga
    14881 caaggtgcag aaaagaaact tttctgaaaa ttaaagaaat ctataatcat gctgagtacg
    14941 aagaggcgtc taaaattctt aagattcaaa atcatgatat agagaatata ggaaaaggta
    15001 aaattgtgat tgctacaggg ggaacttctg atataccagt agcagacgaa gcttatcata
    15061 cagctaagtt tttaggtaat gatgttgata gaatatatga tgttggagtg gcaggtatac
    15121 ataggctttt aaataaaaga cataaaatag atagtgctag agtaatagta gccgttgcag
    15181 gtatggaagg tgctcttgca agtgtaattg gtggactggt tgatgttcca gtaatagctg
    15241 ttccaacttc tgttggatat ggagcaaatt ttggaggatt agcagcatta ctagctatgt
    15301 taaatagctg tgcatctgga atatctgttg taaatattga taatggattt ggagcaggat
    15361 atttagcatc aatgataaat aaactgtaaa tacattgata taaattaaac aaaactatat
    15421 aataaaattt aatttctatt atatttttac tattaaaatt gaggagacat atatgagtga
    15481 aaaaatactt tattttgata taataagcgg tatatcaggg gatatgaccc ttgcaagttt
    15541 attaaattta ggtgtgccta aggaaatttt tttggaggaa attcataaac taagaatgag
    15601 cgatgaattt aatataaaca tcagctcgaa aactgaaaat ggaatagtag gtacaaaagt
    15661 agaagtaatt acaaaagaaa aacatacaca tagaaattta gtggatatat ttgaggttat
    15721 agataaaagc actctaaacg aaaatgtaaa atctaaagca aaaaaaatat ttatggtaat
    15781 tggagaagca gaagcaaaag ttcatggaac tacaatagac aagatacatt ttcatgaggt
    15841 aggggctata gattcaattg tagatatagt aggagcttct atattagttg acttactttg
    15901 tgtagataaa gtttgtgcta gtacagtgtc tgttggttca ggatttgtaa aatgtgaaca
    15961 tggaattata cctatacctg cacctgcaac tatagaaatt ttaaaaggtg tgccaattaa
    16021 attaaataat gtaaatggag aatgtacaac tccaacagga gctgcaatca taaaagtctt
    16081 gtgtgatgag tttgtagatg agcttgaatt ttctccaaag caaattggat atggtatagg
    16141 tcataagaag tttgaagtac caaatatact cagaactgta ttaggtgaaa aaaaaaagaa
    16201 gaattaattt atgaaattag tgcaaacatt gatgatatgt cttcagaaat ctattcttat
    16261 ttgtatgaaa aaatattaga tgagaaagca ttagatattt atacagagag catttatatg
    16321 aagaagaata gaccagcaac aaaagtatca attttatgta atgaatcaga tttagaaaaa
    16381 tttattaaaa tattactttt agaaactagt acttttggag tgagatataa tgcatataat
    16441 agagaaattc tagagagaaa atttgtaaaa ttagatacaa aatatggagt ggttacagta
    16501 aaactagggt attataaagg aaaactagtc aaggctactc cagaatataa tgaatgtaaa
    16561 actatagcaa aaaatgaaga tattcctata aaaaaagtgt ttgctgatat aaattttaga
    16621 ataaaagaag aatttagtga aaacttattg acataagtaa taaattttga taaattaata
    16681 ttcagcctta ttaaataaaa gtaaacagaa aaaaatatat aaaatataaa ctcactcgta
    16741 tatgctcaga aatatggtct gagagtctct accaagatac cgtaaatatc ttgactatga
    16801 gtgaaattat tataccaaaa gacctagtta agtaaatgtg taggctgagt taaactccct
    16861 atgtgttggc tataagataa aggtattttg gaagcctgta tagtaatttc acttttgcta
    16921 gaaattatgg aagttactat acaggttttt tgtgtaaaaa gatttaatat aatttagact
    16981 gtattgtgtt aaaataaata tataaaaact atattataca aggagagatg taaatttatg
    17041 aaacatgaat tagtacttgt aatagatttt ggaggacaat ataatcagtt gatagctaga
    17101 agagttagag aaaacaacgt atattgtgaa atacttccat gtactgcaag catagaaaga
    17161 ataaaagaaa aaaatcctaa agggataata tttacaggcg gtccaaatag tgcgtattta
    17221 gaagattctc caacaatatc aaaagaaata tttgaattag gagtaccaat acttggaata
    17281 tgctatggta ttcaaataat gtctcatgta ttgggtggag ttgtaagaaa aggtaataaa
    17341 caagaaaaag aatatggaaa aacagctata acttatggaa aatcatcatt atttgaagga
    17401 ataactacaa atagtgtatg gatgagtcac acagatttaa tagaaaaagt tccagaagga
    17461 tttactatag ttgcaaatac taatgattgt ccagtagcag ctatggaaaa tgtagaacga
    17521 aacctatatg gagttcaatt ccatccagag gtggaacact gtttagaagg ggacaagata
    17581 cttacaaact tcttatataa tatttgtaaa gtaaaaggtg actggactac agattcattc
    17641 atagaagata aaataaaaga attaaaagaa aaaataggtg ataaaaaagc tttatgtgct
    17701 ttaagtggag gagttgattc ttctgttgct gcagttctta ttcataaagc aattggggac
    17761 aacttaactt gtatatttgt tgaccatggt ttacttagaa aaaatgaagg aaatgatgta
    17821 gaaagaatat ttagagagaa atttgatata aatttaataa gagttaatgc agaagataga
    17881 ttcttatcta aattaaaagg agtttctgaa ccagaagcta agagaaaaat aataggtgaa
    17941 gaatttataa gagtatttga agaagaatca aataaattag gaaaaatgga tttcttagta
    18001 caaggaacaa tctatccaga tgttatagaa agtggacatg gaaatgctgc tactataaag
    18061 tctcatcata atgttggtgg aatcccagaa gatgtagact tccaagaaat tgtagaacca
    18121 ttaagagagt tatttaagga tgaagttaga aaaataggat tagagcttgg tatagaagaa
    18181 ggattaatat ttagacatcc tttcccagga ccaggtctag gaataagagt tataggtgat
    18241 gtaactaaag aaaaatgtga tatattaaga gaagctgatg ctgtttatat ggacgagctt
    18301 agaaaagcag gtttatacag agaaatatgg caagcttttg caacattacc agatgttaag
    18361 actgttggag ttatgggtga tgaaagaaca tatgcttatt tagttggttt aagagcagtt
    18421 acttcttctg atggaatgac ttctgactgg tataagatgc cttatgatgt attagaaaga
    18481 atttctaata gaataataaa tgaagttgat ggagtaaata gagtagttta tgatataact
    18541 tctaagcctc ctggaactat tgaatgggaa tagtaaacaa aaaagtcgta agctttgtga
    18601 tttcaaggct tgcgactttt ttaaaaatgc ttatgatact aaaatgatac tatatattta
    18661 aattatctag tttttgagca acttcaagtt gcttattcgg gtataaatgc gaatatgtat
    18721 tccaagttgt ttctactttt tcatgcccca atctttccgc tatagttaat atatttacat
    18781 ccatattcac taataacgac gcatgagaat gtcttaaatc atgtactcta attcttttta
    18841 cattagataa cttacaacat ctatttagtt ctttagaaag ataactttta gcaaatttaa
    18901 atattctttc attatctttt aagtcataca gcttagatag ataatctttt atattattat
    18961 ataaaaaaca tgggataggg acaacacgtt tacttttaga agttttagga gaagaaacaa
    19021 tatcctcacc atttaatttt atgtaacttt tttcaacact tattttattt tcaaaaatat
    19081 cttttggagt aagagctaat aactccccta gtctaagacc tgtccaaaat aaaatttcaa
    19141 aagctaattt aggttctggt ttcttttcaa attcaataaa ttttttgaac tcttctaatg
    19201 tccaaaaatt catttcatct gcattttttt tgcctattgt acctgctaaa tgagcgggat
    19261 tagaaggtaa gttataatat ttaactgcat aatttaatac agcaacaagc tggttgttta
    19321 ttgtttttat atacgtttgg ctgtaatcac tttttaataa ttcattttgc catttccgta
    19381 tatgtgtggc agtaatttca tttattttta gacttttaaa aaaaggtaaa attctaaagt
    19441 ttattaaata ctttttagtt tctaatgtag ataattttaa tctagaagac atatcgtgca
    19501 tgtattcttc tatgagactt tcaaaactca tatcagtact cattttagat ttattcaaaa
    19561 attctctttc aaactctaga gcttcttttt tagttttaaa acctctctta attttctttt
    19621 tcctatcccc atcgaagtct gtaaaataaa agctagcata ccaacttttt ctttgttcgt
    19681 ctttgtaagc aggcatttta gacaccccca attatgtcat tttaatatta caataattat
    19741 aacatgttag atatgctatc taaataattg ttttattttc ctcttctttt tatttcttga
    19801 agcttgtaat atttacacag ttctaagaag tcttcttcta gtgctataga taagtctaat
    19861 atcgtagata tacctatatc tttgtactct tgattttcta gttttgatat ataacttctg
    19921 ttacgtctca ttttttctgc taattctagt tgtgtcattc ttttcttttt tcttaatttt
    19981 ttcaacattt attaaaatcc accttttaat tatttttatg acatttttat aattaatagt
    20041 ttgtgtaatt tcttgtaaaa aatgttcctc attagaacat tttttggtgg aaaaatgtgt
    20101 taaaatgtaa ataagaaaag cactttcaaa gcactataaa aatagtgctg aaatagataa
    20161 acaaataaaa gtaataagga ggaaagatag attctagagg gaaatcatta tcatttttaa
    20221 gaacgtatgt tcagttggtg ggatagaaaa atactttaca ggggatggtt tatttgaatg
    20281 agaatgaaaa tatgagttta tatgtcagta agttaaaaga agtattgaaa aataattcag
    20341 aagagtataa aaaaataaga gctaaaataa atgaaattta taatctaaat gaaaagaaag
    20401 aataaaaggg ctttagctct tttattcttt cttagtatta actgctttat aataagcatt
    20461 atctatcatt ttttttattg catttttatc atctgaatct aaagatgcta atctatttat
    20521 tagttcgtta acttcattat cttcttttga gttaactttt tttaataatt catcatcttg
    20581 ttgagtgtta ggttgtattt tacctaattt agttaatagc tcctttaaag ttaaatttaa
    20641 tccaaaactc attttttcaa gcatatctaa agtaggttca acagctttac cattccttgg
    20701 gtctctgttt ttttctatct tatctatata tgtatggctc acaccacaaa gattagaaaa
    20761 ttctctaaga gataagttat gttcacttct atattcttta ataatttctg ctaatgtttg
    20821 cataatttca cacctcatct aaataatagt tgacttaatt atatcagaaa aatgtaaact
    20881 attgttaaca aaaataaaaa tttagtaaaa aataattgac aagagtaaaa aaaagacgta
    20941 tactatgatt aacaaaaaag ttataaataa agttaggagg gattaatata aaaaataatt
    21001 tacaatatat tagaaaagaa tctagaatat cacaaaaaaa attcgctgat aagataggta
    21061 tatcaagacc gtatttatct agaatagaaa acggtaaggt taatccgagt ttagaaatag
    21121 cgcataaaat ctcgctcgaa acagggaaaa caataaatga aatttttttt gattttactg
    21181 taaatcatag taaactataa ggttttaatt gtaccttgaa aactaaatat aaaatatttt
    21241 aaaggagcaa gtatatgaaa aataaagaga aagaaatatt cattagagga attttaattg
    21301 gaatctttta ttttttagga attgtattta gtaatacttt ttttaaataa taaatagaag
    21361 gggtaggtga aataccaact cccaaaatta aaataacact ttggaagtta aatatagaat
    21421 attcaaaaga ggtgattaga tggaaataga gcaaacaaca atacgcctgc taaaaagcag
    21481 acgaaagttg aatattaatt tctattttgg ttaggcgaaa gacctaacaa aaaatctgta
    21541 gatacattaa gtttgttaga aattaatatt aaagtttcta tatttggctc tcttgaaccc
    21601 gattcataat attgatatgc acgttctgag ataccaaaca aactagcaaa ttgcttttga
    21661 gtcatattca tttgctttct tactagctta atattatctt tgaatttagt cataactaca
    21721 cgctccaata aaaaaataaa taaatttctt gacacgaaca atattagcgt gtaatatata
    21781 aaacatagca cgaacaatat tggcgtgtta aataaaaagg agagggtgaa aataaataat
    21841 aatttaaaac tacaacgtga aaaaattggc ttaacacaat tagaggttgc tcaaaaagcc
    21901 aaaataacag agagaagcta tcaatattat gaagctggcg aacgtctccc aaacattcgt
    21961 acagctttaa agatagctag aattttaaat actaattgtg aaaaactctt taatgaataa
    22021 tagcataaaa ggactcagat tacaagaaag gagctttaga tatgaataat ttgcaactta
    22081 acaataaaaa tacaataaca acattagaaa ttgctgatat gttagaaatc agacactggg
    22141 aagtcttaag gaaattagaa ggaacagaaa aaacaaaagg aattattgat attcttaacg
    22201 acaacaattt tgttgtggtt gattacttta taaaatcaac atatttggat tcaaaaaatg
    22261 aaagtagacc ttgctataat gtaacaaaat taggttgtga ctttctagca aataaattta
    22321 ctggagaaaa aggtattatt tttacagcta gatatgtaaa aagatttaat gatatggagc
    22381 aagagctaaa agaacaacta aagtgtagtt atatgataga tgacccaatc gaacgtgcta
    22441 agtgctggat aaaggaacaa gaagaaagcc aatctttaat taagaaaaaa gacaacataa
    22501 tacaattaca acagcctaaa gttgagaaat gggaaagatt tttaaataat gaaggtctta
    22561 caacaataga agatttttca aagacactag caataaaagg ttatggaaga aacaatatgt
    22621 ataaattatt gagagaaatg gagtatttaa agaaagataa tagcccttac acaaaatatg
    22681 tagagcaagg tttatttaca agaaaaccaa gtggaacaca catagaatac gggaaagtaa
    22741 tagaagatta caagacattt ttaactagca aaggtataga taaaataatg gaaaaacttt
    22801 tgaaacttgg agttataaag caatcaagca atgtatctta tgttgagcaa aagcagttgt
    22861 caatattaag ttaaataact gtagcacttt agaaattaaa tacagaatat tttgaaatat
    22921 attgttttaa ttaattatta actaggaggt tgttaaatac atgaaaaata atacaagtga
    22981 ttcaagagta agatattttt gcaagtgtcc atactgtggg tttgataatg aggtagaagt
    23041 taaaaaagga ttgaagccta agatatgttg tatgtgtaca aaagaagttg agtatgaaaa
    23101 actggagcga caaagtgatt cagaaaatac cgaaattaaa ggagtgtgta attaaatgaa
    23161 aataatatta gagtttgaaa atgtagaaga gtacaaagat ttcataaaaa aatctgctca
    23221 atggccaaac ggccaagaac agattaaaat aaacattaat cctgacataa agaaaattgt
    23281 tccagataat cttctctatc aactggaaaa aaacctacaa tttgctcagg atataatatc
    23341 atctcattga agtttaattg tatattagga tatttaacaa taacattttt taaagcaatt
    23401 gtattagtgt tgtttacaag ttgaagttcg tctttcattt tattaacaag gtcaactttt
    23461 agtttattta aatctttatg tatacccgta gaatcatttt tgaaatctgg ttctccaatt
    23521 acaaaaccaa aattagtcaa tattataata tctttgattc ccatagaatc acaataagaa
    23581 ctaagtattt caatatctac atagtttcct gataattttt tcattgatat atagcacctc
    23641 catttttaat aatatgggac ataatccata tttatattat accatgtaga actgaggtga
    23701 ttaagtgtta ataggcgaca atataagtca aatactaaga aaaagaaata taaaacccta
    23761 taagttagca aaggaattag aaatagatga aagtggatta tacaaaatta tacgtaatga
    23821 aaagaaaaat ccaactataa atactttaat aaaaatagct gattatttag atgttacatt
    23881 agacgaacta gttggaagat aaaaattaaa tacagaatat tttgaaagta tgtgttttag
    23941 atgacaaaag tagtagctaa agaacaatta ttttatagag caaaagatgt agctaaattc
    24001 ttagatatat gcgaagcaac tgcatacaaa ataattgcgg aattaaatga agaattagaa
    24061 aaagaaggtt ttaagacttt ttctggcagg gtatcaattg cttattttaa agaaagatat
    24121 tgttataagc caagaaaggg ggtgatttag ttgaatgtaa gagtattaat agcttatata
    24181 cagttctgta agcaatataa taagaaagca agttttgaag gacttaaaaa atataataag
    24241 gggatagtga tatgaaaata cttaaagtaa aatttcctac aaaaattctt aaagcaggta
    24301 aagcaatcag agtaacatgt gcaagatttg gttttgaaag cgattgtata ataactcaat
    24361 cacaagaatt tgaattaaca gtagtttatt ttgataaaga attagatgat ttagtacaat
    24421 cgtctataac tatagatgat gcaataagct atgattattg cattgaaatg ctaaattaag
    24481 ggggattaat catgaaaagt ttaattatag tgagaaatgc agtagagcaa caactaaata
    24541 gagctaattt agaaataaat aaaaatgagg aactttatac aaaacttaga aaaaaagaag
    24601 aaagaaatat attagaagaa attgaattga gtaatgcttt aagagaaaaa agtgtaaacg
    24661 aaagattaaa aatatttgct gagtcattac tagaaattat agatacacaa attgaaataa
    24721 aagaatatga agaaagtgag gattacaaga tatttcaatt aattttagaa gaacttgaaa
    24781 gagatagacc tatagatgta cagatataag aaaagagcct atagcgaggc tcaattcaaa
    24841 aatagaagta aaaaatttaa ttaagctaat tatagcacaa acggagggaa attatgagta
    24901 ctttatatga attaactaca gatttattag aaatagaaga aggtttaaca gaaataacag
    24961 gaaatgaagc tgaaaaacta gaggaaataa aagaaataat aaaacaagag atacaaaata
    25021 aaaacactag gatagtttca gtgataatag atattgatag tgatataaac tctttagact
    25081 tagagattaa gagaatgcaa gagttaaaga agattaagaa gaatagtctt gatagattaa
    25141 aaagtaacat aaaggaatgt atggaactgc ttggtattaa aaaggtagaa acatttttag
    25201 gaaatataag tataagaaag tcagcaggta gcttagtcat agaagatgaa gaaaagatac
    25261 ctgctatata taaaacagta gagcaagttg taaaagtaga taaaaatacc attaaagact
    25321 ttatcaaaaa aggtcatgag gttgaaggtt gtaggattga atatggaact acactaacaa
    25381 ttccaaaagc taaaaaagag taggtgagga ccatggaaac taataatgtt tatataaaac
    25441 ttgtaaatat acagagtact ttaaaagctc ctaaaagtca atttaatagc tttggtaaat
    25501 acaactatag gagttgtgag gatatactag aaggtttaaa gcctattcta aaagaagaaa
    25561 aagcattggt tgtattggat gataatattg ttcagatagg aaatagattt tatgtagaag
    25621 ctacagcaac tttaatagat gcagaaacag gagagaaagt atctacaaag gcattagcta
    25681 gagaagatga aactaaaaaa ggtatggatt tagcacaagt aactggaagt gtatcaagtt
    25741 atgcaagaaa gtacgcttta aatggattat tttgtattga tgatacaaaa gatagtgatg
    25801 caacaaataa acatggaaat gagcagaaaa aaaaagaagt taatgagagc gaattaaata
    25861 tactatattc gctaggagaa tctatagaaa aagataaaaa tagagttgat agtgaagtat
    25921 ataagaagtt tggaaagtta gcagtagatt tgactaagca ggagtatgag aaagttttaa
    25981 atggatataa gagcatttta gagaagcaaa aacaagagta ggtgatagta ttggggatta
    26041 taagagtaag caaagacaaa gataatccat atgtagtttt aaataaaact tgtttggaag
    26101 atgtaaaatt aagctggcaa gcaaaaggct tacattcata tctgattagt aagcccgacc
    26161 attggaaaat ctatgttaat gatttatata aaagaagtaa aaatgggagg gatgctacag
    26221 caaatatttt aagggagctc atagaaaatg gatatataac aagaacacct tgtcgagatt
    26281 ctaatactaa taagatgctt ggaggatatg attatcaagt atatgagata ccacttgaaa
    26341 atcctcagaa gctaaaatcc cgaaaaactg atttcccgga aaccggattt cccggaaacc
    26401 gggtttctcg gaaaccggaa aacacggaag tagtaagtaa tgactttaaa gtaaataatg
    26461 atattactac tattgttatt aatgaacaac ctcaacaaga tgaaaccacc tatataaaaa
    26521 aatactttga aaaatatata ggtgtgatta ctcctaataa ctttatagag ttaatgagtt
    26581 acttagatga tggaatggaa gctgatgtaa ttataagagc tattgatgaa gcaatatcaa
    26641 atggagttaa gaattataag tatgtaaaga caatattaaa caattggata gaagcaggtg
    26701 taaaaactaa tttagaactt acagagtatc aaaatgagtt tgagaggaag aaaaagagta
    26761 aacaggagaa gaaacagtct aatcataaaa atgtgaatac tcctaatgtg agtaaaaata
    26821 agtttcataa cttcaatgaa acctttactc aatattcacc tgacgaacta gatgacataa
    26881 ttaagaaaag tcaaaaggtt aaatttaaat aaaattatac ttctaggaag taaatatcaa
    26941 tatattactt cctagaaagg ggaggtataa aatggcgaga atatatgcac aaagaagtgg
    27001 ttctttaaac gaacaagata gattggagtt attaagatta cttgggaaag ctggatatac
    27061 agtaaaggtt gctagagaga agcaaaatag caagacaact tatacttact ttgttgagta
    27121 tacagaagaa caggaagaaa aatagaaggg ggctagttaa atgaatacaa taactttagt
    27181 tggaagatta gttgcagatg cagaattgaa gtaccttcca aattcaggta ctccaaaaat
    27241 aaccttttca atggcagtag atagaaggtt taaagataaa aatggaaata aaataactga
    27301 ttttattcaa tgcgagcaat taggaaaaca tgtagagaat ttagtgcaat atcttgttaa
    27361 aggtaagcct atatatgctg ttggagagtt aaatatatat aattacaaag atgaaaatgg
    27421 ttgctggaaa tctattacta aagttaacgt gaatgcttta gaattacttt ctagtaaaag
    27481 tgatagtaat aatcataaag agcaacagga atatatacca ccaggactgg acccacaagg
    27541 ttttcaagca atagatgatg acgatatacc tttttaatta agttaagtag tctagggagt
    27601 aattatacaa tattacttcc tagaagttaa aaaatattgg aggtctaaga gtgaaatatg
    27661 agtgtgagaa agtgttctta gaatgcgata agggaagttt tgagataaat gatacaagaa
    27721 ctgaagaagt aacatttgag ggtacagaaa tagacaatcc atttaaacga gtaaaatatg
    27781 aaggtaaagc tacttttgaa atagtatctg gatgggagta tctacaaaga gaaatgttgt
    27841 ggtttaagat attgcattta tcagcagttg tagcaaaaat aatgcaatat aaaatgttag
    27901 gtattttaat aagggagatg taataaaatg gctaaaattt ggatggatgc aaatgaagtt
    27961 ctaagtaaaa ctatggattt agaagatatg tttgaactta atttaagagc aataagaaag
    28021 agaaatgaaa aaataaaaaa tgaaattgag aaacaaataa aatatgaaga ttcagaaata
    28081 aaaactacaa atcgtggtgg accagggaaa attataaaaa ttttcaatat aaatactgga
    28141 gaggtaaaga ttcttaagag tgctgaagaa gcaagtagat atataaaggt tagtcgtagc
    28201 tatgcaagtt atttagctag agaaaataaa tcaactgagg atggttggaa agcagagtat
    28261 attcaagagg tgtcagatgg tattagcaaa tgtggaacaa gtaattaagt tagctgaaaa
    28321 gatattaaat aagaaaaagt gttctgttaa taaagctatt gatatagcta taaaaatatt
    28381 aagtaaatat gagtgcgagg ggatgattag aaatgagttt aattaagtat agaggttatg
    28441 attttgagaa tgagaagtgg atttattcag caacaataat gtggagtgat gcagtagatt
    28501 gtttatttat gctaaaagag aattgcgaat ggcaaagagt atgtaatgtt ggagtatgct
    28561 ctggagaatg ggctagaaac aatcaagaaa tttgtgaagg ggatatattg aaagaatatg
    28621 ataatttcca tgatacaagt caatatggaa ttgtaaaaag aagttttaac agcattaaaa
    28681 tatatttgga atggcattat ttaaagaaac ttgaaggaga atggatagag cttataaata
    28741 aaacagaaat atatcatagc agagattaca aaatagttgg taatgaatat gagaacttag
    28801 aggaagtcag aacagagttc ttagaacgta aggagatgtt ggaaaatgaa catcttggct
    28861 agtgtgctac tgataggaat tagttttata gctggtagag tttatgagta tagattgaat
    28921 ctcaaagagt gtgaaaattg cgacaataaa agaggtgtat aagaatggat gatagattag
    28981 aaatgataaa tgcttctgta aattatatac agatgatatg tgaaagttca aatatagcta
    29041 ttatagcaga gcatgaccga gttagaatat tggatttaga aactaaagaa aaatatgatt
    29101 tattaaaaaa taaactcgag gaaatgttag aagaaatata agtgaaaata tctaattaaa
    29161 acagtttaga gagttgcaaa atgtcttttg gtataaatta ttgttgaagt gttttgtgac
    29221 tctcaaaaat gaaaataagg aggcgttgta ttgcttacat ttttagattt attcgcaggg
    29281 ataggtggct ttaggctagg gatggaaaaa gcaggacata aatgtttggg acattgcgaa
    29341 tatgataaat tcgcaaattt aagttataat gccatgcaca aaccgaagga ggatgaatgg
    29401 tttgaaagag atattagaga aattagaaca gaaaatatcc caagagcaga tgtctggtgt
    29461 tttggattcc catgtcaaga catttctgtt gcagggaaac aatttggatt cagaggagaa
    29521 cgttcaagtt tattttttac agttacaaaa cttattagag aactcaaaga agaagataga
    29581 cccaagtatt tacttattga aaacgttaaa aatctactta gtgttaatgg aggatttgat
    29641 ttcctcaaag ttctcgttga actggatgaa atcggctatg atgcagagtg gcaagttctt
    29701 aattctaaaa acttcggagt accccaaaat agagaacgaa tattcattgt tggacatttt
    29761 agaggacgaa gtacacgaaa agtatttcct atcgaaagaa aaagtggaaa aaatcttgag
    29821 caactaaata atccaactca tagtacaaat agaatttatg atgcagttgg aattgctaga
    29881 tgtattagaa gtcaggcagg aggtggaggt gctaaaacag gtctatactt tatagactta
    29941 aataaaaact ctaaagtaac aataaatgct agatgcctta aagcaaaata taatgcaggt
    30001 gtgacaaata gaaattgtga taatagtgga gttttagtta atgcagtttt aacgcccgat
    30061 agggtaaata aaagacaaaa tggtcgtaga attaaagaaa gcggagaacc aatgttcaca
    30121 ttgacagctc aagataaaca tggaattttg aaaaatggag atataagaag gttaacacca
    30181 aaggaatgct ttaggttgca aggatttccg gataaatatt acgaaagagc agcaagtgta
    30241 tgctcagata gtcaactgta caagcaagca ggaaatgctg ttactgcaaa tgttgtatat
    30301 gaaatagcaa aaagaatggg ctaaaagttg caaaatgtct tttagtatga atatttttga
    30361 agtgttttgt aactctcaaa aatgaaaata aggggtggga taataaaatg gatttaaata
    30421 aaattatgaa tgatgcacta gtggaaatag aagaaagtgg ctttgtagag gaaacagtta
    30481 aaaaagaatt agaagaaaca ataaaaagag ctgtgaatga aatctttgga agctatagta
    30541 aattcagaaa aaatattgaa caacatttag gtgaaaatat aaatataaat ttagatgaat
    30601 tagatataca aaaatacaat ttacttgtgg caaatgttgt aaaagaaaag gtagatgcta
    30661 cgatgaaggg gcaaggtatt gagcatttaa aaagaaactt ggataacatg cttgtaggtc
    30721 ttaagaaaga atacaaaatg agtgaattat tagaagaatt gaaagaagat aaatacaacc
    30781 ttgacgagta cccatgtgat gaagactgta taacatttat ccttgaatct aattttaatt
    30841 cagaatgggc aagatggata gactttgatg agtgtcctaa tgtaagcaga aataattgtg
    30901 aacattctat actattaaga aacgatggaa ctattgcagc atttagatac caaaacagag
    30961 aaatatcatc aaaagatata atgaacggat ttggtaattt tggggattta ttatttaaga
    31021 tatatgcaca taattcaaaa attatcttag atttagggaa tgatgtagac gattatgact
    31081 taacaaatgg agaagattat tagaataggg gtgaataaat gtcaaagtat gtattgagat
    31141 ggcaaaaagg attattacta gatgaacgca agataaatta ttcttgtgga agtatagaaa
    31201 atttaaaaaa gaaagctgaa ttattagcta aagatgacaa gatattgctt gtaacaatag
    31261 ataaagttga agaagttata aaagatacta gaagtcaaaa aatggctgaa tattattgtg
    31321 atggaggaat tgaaatatga taatacacaa atttataata catgttttag ataagaatag
    31381 cga
//
LOCUS       DACXMZ010000017        30776 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.017, whole genome shotgun sequence.
ACCESSION   DACXMZ010000017 DACXMZ010000000
VERSION     DACXMZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.017"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            201..3749
                     /locus_tag="JD016_03165"
     CDS             201..3749
                     /locus_tag="JD016_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAU4998631.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKCSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSKGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            3934..5868
                     /locus_tag="JD016_03170"
     CDS             3934..5868
                     /locus_tag="JD016_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAU4998632.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5974..6969
                     /locus_tag="JD016_03175"
     CDS             5974..6969
                     /locus_tag="JD016_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAU4998633.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMVAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6941..7726
                     /locus_tag="JD016_03180"
     CDS             6941..7726
                     /locus_tag="JD016_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAU4998634.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7714..8412
                     /locus_tag="JD016_03185"
     CDS             7714..8412
                     /locus_tag="JD016_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4998635.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8535..9008
                     /gene="ybaK"
                     /locus_tag="JD016_03190"
     CDS             8535..9008
                     /gene="ybaK"
                     /locus_tag="JD016_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAU4998636.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9205..10044
                     /locus_tag="JD016_03195"
     CDS             9205..10044
                     /locus_tag="JD016_03195"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAU4998637.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10057..10605
                     /locus_tag="JD016_03200"
     CDS             10057..10605
                     /locus_tag="JD016_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HAU4998638.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10668..11852
                     /locus_tag="JD016_03205"
     CDS             10668..11852
                     /locus_tag="JD016_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAU4998639.1"
                     /translation="MMSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVA
                     EPCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFA
                     DVDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFH
                     DDQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILC
                     DRSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVE
                     SMSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDV
                     KAKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVN
                     RI"
     gene            12144..12533
                     /locus_tag="JD016_03210"
     CDS             12144..12533
                     /locus_tag="JD016_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU4998640.1"
                     /translation="MMEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNIT
                     AYDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQ
                     PGVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12683..12946
                     /locus_tag="JD016_03215"
     CDS             12683..12946
                     /locus_tag="JD016_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAU4998641.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13075..13917)
                     /locus_tag="JD016_03220"
     CDS             complement(13075..13917)
                     /locus_tag="JD016_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAU4998642.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14212..14946
                     /locus_tag="JD016_03225"
     CDS             14212..14946
                     /locus_tag="JD016_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU4998643.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            14956..16089
                     /gene="nagA"
                     /locus_tag="JD016_03230"
     CDS             14956..16089
                     /gene="nagA"
                     /locus_tag="JD016_03230"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAU4998644.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGEVVYKNI"
     gene            16188..16937
                     /gene="nagB"
                     /locus_tag="JD016_03235"
     CDS             16188..16937
                     /gene="nagB"
                     /locus_tag="JD016_03235"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAU4998645.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            complement(17069..17488)
                     /locus_tag="JD016_03240"
     CDS             complement(17069..17488)
                     /locus_tag="JD016_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998646.1"
                     /translation="MKKALRYLLFLIILLLMYFLAVKLNYDFYTQFYTGYMQDFKRYI
                     FINLISSGGIGLLLGTELLIREYKKDGSWYIDIPRLLLLCFPSFLLSLMPVFFFMFPV
                     GNIPIIGNFIMLDRIPLNIIIFNILFGYFLITSFRKK"
     gene            18044..18748
                     /locus_tag="JD016_03245"
     CDS             18044..18748
                     /locus_tag="JD016_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4998647.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            18846..20237
                     /locus_tag="JD016_03250"
     CDS             18846..20237
                     /locus_tag="JD016_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU4998648.1"
                     /translation="MLSILCLIIIVFMIILVSFNVFLDTYIKTTANEELTKSTEIVEH
                     MDSNFRPIKPPQDDGKLPPKVLSNFMRNVQDKVRMAETQSDADAMVVDSKYNLIFPTR
                     EDDFLKNVDEMELIRECIQNEKLDLNSDANTRISTRNKDYYISTVKITSIYGEQDKYL
                     ILFIDISKTLNLAQKINIVLISVMCFAGILAIFTAVILSEKIAKPIKELCEFAKKMGQ
                     GDFKRTYFDFSDKELVELSTVMNKSAEYLDKYDNEQKIFFQNASHELRTPLMSIKGYA
                     EAIKYNVIDSKHASDIILEESDRLSDMVEDLLYISKIDNITKDFELVECDLREVLSNC
                     GARQNVRAINKGIKFIYEFDEEMVLFECDEKNISKAFMNLIENALRYAKSEIKIVCKY
                     NQKNIVVIIEDDGIGIKKEDLPHVFERFYKGVGGNHGIGLSIVKSIVNKHGGRIYVEN
                     GKKGAKFTILFKL"
     gene            20442..22055
                     /locus_tag="JD016_03255"
     CDS             20442..22055
                     /locus_tag="JD016_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAU4998649.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(22212..22382)
                     /locus_tag="JD016_03260"
                     /pseudo
     CDS             complement(22212..22382)
                     /locus_tag="JD016_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432172.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(22418..23248)
                     /locus_tag="JD016_03265"
     CDS             complement(22418..23248)
                     /locus_tag="JD016_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAU4998650.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIRPKGLYVTA
                     YHKGSYETIYKTYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            23321..25048
                     /locus_tag="JD016_03270"
     CDS             23321..25048
                     /locus_tag="JD016_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4998651.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            25085..26947
                     /locus_tag="JD016_03275"
     CDS             25085..26947
                     /locus_tag="JD016_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4998652.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            27280..27969
                     /locus_tag="JD016_03280"
     CDS             27280..27969
                     /locus_tag="JD016_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HAU4998653.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            28074..28760
                     /locus_tag="JD016_03285"
     CDS             28074..28760
                     /locus_tag="JD016_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HAU4998654.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            28777..30684
                     /locus_tag="JD016_03290"
     CDS             28777..30684
                     /locus_tag="JD016_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HAU4998655.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKITVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
BASE COUNT        12163 a         3209 c         5386 g        10018 t
ORIGIN      
        1 tataattttt aatattaatt aagaatgatg aaatatgaat aaagatttta caaataaatc
       61 ttaagctatc ttatttaatt ctaatacagg aagaaggtaa atgtatattg aaaaaatcat
      121 agaaatagaa gtaaacaatt taaataaata caaaggtttg caaaagaagt aatataatta
      181 tatacaatga ggatgtgagt atgatgaaca gactaaagcc aagaatttta ggtattagta
      241 tcttgtttat tattataatt attttaacat gtggagctgt tttcaagttt aaatttaaat
      301 tagataataa attaaaagaa accacacata catatttgtc tgagtttaca gaacacaata
      361 taattaatct caagacaaga cttagtggtc aatttgacat gcttgaatct attgctaact
      421 tcataggaaa cttgaataat ataaatgatg aaatggtgat tgatttaatg gattctgatg
      481 taaagtgcag ttctttaatt agaatgacta tctctacatt agatggggaa tgttattcta
      541 atgatggaat tacccataat gttcaagata gagattattt taaaaaagct aaaaatggag
      601 aaaagaatat atcagaacct ttaaaatcta ttgttaataa taaagaaata atagttttat
      661 ctaccccagt ttataaaagt gggaaaatag ttggtgtttt aagtggaatt tatgattcaa
      721 aaaagttaaa tgagttatta ggattatctg catttgatga aaaagcagat gtttttgttg
      781 caaagaaaaa tggagatata gttgctcaaa aaacaaataa aaatagaaag acgctatcat
      841 gtatatttca gctttttgac aaagataatt gtgatgaaga tgattgtcta aaaactataa
      901 aagaaaatat gaaaaatgat aagtctggat attttaatta taacttagat aatgaaaaat
      961 ttatgttaaa ctatcagcca cttggagtta atgattggta tgttttttct gttgtgccaa
     1021 ataaaatagt atctattcag tcagagaata tatcttatga tgtatatatt tttgcattag
     1081 aaatactact agttatattg atttttataa tatatatagc atgtattata aataataaca
     1141 ccaaaattat tataaatgaa aaagaaaaat ttaaagtact cactgataat atattaggag
     1201 gggttaaaac ctgtttaaaa gatgaatata tgactatgac atatgtaagt caggggtttt
     1261 tagagcttac aggttactct tgtgaagatt taaataaaat atttaaaaat agatttaaag
     1321 acatgattta taaagatgat ttagacaagt ttaattgtat tattctaaat caattagaaa
     1381 aacaagataa aattgaagtt gaatatagaa ttgtaagaaa agatggaagt ataatttgga
     1441 ttttggagag aagtaagtct ataaatgata ataaaggaag tgagtgtatt cagggagttt
     1501 taactgatat aacagaactt aagaaaatac aagaagaatt agattcaaaa agatttgaaa
     1561 tacaggctat caatgatagt attgttggtg gaatagtgat aactgagata aatgatgatt
     1621 ttgatagtat atatttaaat gaaggttatt taaatatgat tggatataca cgcaaacaac
     1681 tggaagatga gctaaataat aaattaatca aacttatttt taaagatgat agaacagaat
     1741 taattaatag tataaacaaa caactcaaat taagtgatac atttgcatct gaacaaagag
     1801 ttgaaaaaag ggatggcact attatatgga ttttattaaa aggaagaaag atgatttatg
     1861 ataatggaaa atctgtactt tgttgtgtta ttgtagatat tacttctgct aaattattag
     1921 agcaagaact taaaaagagt gaagaacgtc atgaaatcat tctaaagcag acgcaaaata
     1981 ttataataga atgggatata gaaaaagata aaatgaatat atcacctaat tggtataata
     2041 agtttaaata tgaccctata actgaaaacg caacaaaaaa tataattaaa tcagtagata
     2101 tatatcatga tgatatggat aagtttattt cattaattaa acttacttca aaaggtagtg
     2161 aatatgaaga gtgtgaaatt agacttagaa ctagagatac tatttacgct tggtttagta
     2221 taaaggcaac aactttattt gataataatg gtaaacctta taaagtagta ggaagtttga
     2281 ctgatattga tgaatcaaaa cgagaagctg aaaagttaaa agaaagggca gaaaaagacc
     2341 ctcttacaga tttgtataat aaaaaagcat cacaatcact tatagaagag tacataacat
     2401 tggataaaaa aagaaaaggt gctttaatga ttattgatat tgatgacttt aaaggaataa
     2461 atgataattt aggacattta tatggggatg ctgtattaag tgaaattgca aatgacctaa
     2521 taaatctatt tagagcaacc gatatagtag gaagaattgg tggagatgag tttattgtat
     2581 ttataaaaga tatttctgaa acaagagata ttataaataa agcagaagaa ttggtaaaaa
     2641 catttgaaag gtctttttta ggtaaaggac gaaattataa aatatcatta agtgtaggaa
     2701 ttgctaagtt tccacaagat ggtatggact atatgacact atttagaaat gcggatatag
     2761 ctctttactc agccaaaggg aagggtaaga attgttatac attgtatgat aaaaaaattg
     2821 ataaagtcca atatataact aatacaagaa atgcaggaga tagtgataat gtagagatgt
     2881 cttttaaaga acatatcaca aattatatat ttgatatact atatgaatca agtgatatag
     2941 aagtggcagt aaatttaatt ctaagtattg taggtaaaca ctttaatgtg agtcgcatgt
     3001 atatatttga aaattcagat gataatcgag tatgtaaaaa tacatttgaa tggtgtaacg
     3061 aaggtgtaac ctcggaaatt aataatctta aaaatatttg ttacaaggat ttaggcaact
     3121 atcttttaaa ttttaatgca gatgggttat tttattgtaa tgatattgaa aatttaggta
     3181 gtgatgtgaa aaaagtactt aagccacaag gcatacgttc aatattacaa tgtaaaattt
     3241 tagagggtaa tgaatttaga gggttcatag gatttgatga atgttcatat aatcgtatgt
     3301 ggactaatga tgaagtgaac actttgacat ttatatctaa aacgcttagt atgtttttat
     3361 taaaaatgcg tattcagagt aagctagaag agtctttaac aattcaaaaa tctgtgatgg
     3421 ataatatgga tatatgggca tatgttgttg acaggaatac ctatgaatta ctatttatca
     3481 ataaaaaaat gcttgagtca gcaccaaaca ctcatgtagg tgatttatgt tataaagcga
     3541 tatggaataa tcaagataaa ccttgtaaaa aatgccctat gatatattta aaagatggtg
     3601 atgatactta taaatcattc atgcataata aaaagtttga ggtacaggca aatataacta
     3661 caactacttt gaaatggttt gatggtaaag atgcatgttt aatatttgga agtgaaataa
     3721 ctagtattga agctgaagaa aatacttaaa atactgtgtt atatatatac ttaaaatcgc
     3781 aatttgaatt tattagactt aaaatgtcta tataaatcat ttaagttgtg gttttaagta
     3841 ttttttatga tataattaag cgttaagaac aaaattaaaa gatacttttg tataaaaaaa
     3901 tatttgttaa atatatattg agcaggtgat aatatggaaa ataagttttt accaataagc
     3961 aaacaagata tgatagatag aggatgggaa gaattagatt ttgttctagt tacaggagat
     4021 gcttatgtgg accatcatag ttttggaaca gcaataatat ctagagtttt agaaagtgct
     4081 gggtataaag ttggaataat agctcaacca gactggagaa ctacagatga ttttatgaag
     4141 ctaggaaaac ctagacttgc atttctagtt aatgcaggaa atatggattc aatggtcaat
     4201 cattattctg taagtaaaaa gcatagagaa aaggatatgt attctccagg tgggaaaatg
     4261 ggttatagac cagatagagc tactatagta tattgcaata aaataagaga agcatacagt
     4321 gatgttgcaa tagtaatagg tggaattgaa gcgagcttaa gaagatttgc acactatgat
     4381 tattggagtg ataaggttag aaagtctatg ttaatagata gtggagcaga tttattagtt
     4441 tatggtatga gtgaaaagca gattgtagaa gtagccaatg cacttaatga tggatatgac
     4501 ccaaaataca ttagacatat tgatgggaca tgctatattt cagatacatt ggaagaaata
     4561 tatgataaat atatattaat tccatcatat aaagaaattt gtgaagataa aatgaagtat
     4621 gtagaagctt ttaaaattca atatgatgag caagacccat ttagaggtaa tataattgtt
     4681 caacctcatg gaagtaagta tctggttcaa aataagccag aaaaaccact tagtagagaa
     4741 gaactagatg aagtatatgg attaccatat cagaagacat accatcctgt gtatgaaaaa
     4801 tttggaggaa taccagctat agaagaagta aaatttagta tagtaagttc aagaggctgt
     4861 tttggtagtt gttcattttg tgcaataaca tttcatcaag gtagagcagt gcaaagtaga
     4921 agtgaaaaat caattattga tgaagcaata ggaataacaa atttagatga ttttaaaggg
     4981 tatatacatg atgttggagg tccaacagct aattttagaa gaccagcatg taaaaaacaa
     5041 attactaaag gtgcttgtaa aaatagacaa tgcttatctc catcaccatg taaaaattta
     5101 gatgcagacc acagtgaata cctacattta cttagagctg taagaaaatt acctaaaata
     5161 aaaaaggtgt ttgtgcgttc tggaattaga tacgattatg taatggcaga taagaacaat
     5221 aaatttctta gagagttgat agagcatcat gtaagtggtc agttaaaggt agccccagaa
     5281 catatatcag aagaagtttt aaagtatatg caaaaaccag caggaaaaac ttatgataaa
     5341 tttagacaga agttttttgc tataaatgaa gaattaggta aaaagcaata tttgataccc
     5401 tatttaatgt ctagtcaccc tggttctacc ttaaatagcg ctatagagtt agcagagtat
     5461 ttaagagata ctcactacca accagagcag gttcaggatt tttatccaac tccagggact
     5521 ctatctacaa ccatgtttta tacaggtatt gacccgctta caatgaagcc agtttatgtt
     5581 cctaaatcaa aacgtgacaa ggcaatgcaa agggctttat tacaatatag agcacctaga
     5641 aattatgatt tggtatactc tgcgttagtt gaagcaggaa gagaagactt aattggtttt
     5701 ggacatagat gtcttataaa gccaaaaaat gaaaagcctt attttaatag aaataattct
     5761 aaaaaaaatg tatctaaagg aacaaataaa aacaaaaaaa ctaatacaaa taacagaagt
     5821 aataaaaatc aacaaaaaag ttcaactaaa aaaaagaaaa aacattaaag tataaataaa
     5881 aatacttaaa aaagaaggat tattcgataa attaacagaa attagtataa ataaatataa
     5941 ttattttaat gacgttacct ttgggaggaa gagatgaaaa agtatatttc tataatatta
     6001 ttagtggtgt tgactatggt tgttttggta ggatgttcac caggaaaaga taaccctaaa
     6061 aataaggagc tatcagttgt caaacaagta aaagtagctg ttccagatgg actaccagca
     6121 gtggcaatag ctaaacttgc aaatgaaaat ccagagatta aagaaggata tgaaacagtg
     6181 tatagcattg aaaagacacc tgaggcgatt tccactagag tgatgaaaaa agatgcagat
     6241 atagctatag ttccttcaaa catggttgca atagcgtata ataaaacttc atcatataat
     6301 attgttggaa ctgtaggaat gggttcactt tatttggtgt caacagaaaa tataaaagat
     6361 tactctgatt tagttggtaa agaagttgga tgtactggaa aaggacttac tccagacata
     6421 actataaaaa gtcttttgtt acaaaaggat ataaattatt cagatataaa atttaattat
     6481 gtaaattcag caagtgaact ggtaccatta ttggctactg gtaaagttaa aacaggtatt
     6541 gttccagaac ctgcacttag tgctttgatg tctaaaaatc cagacataaa aattataaaa
     6601 agtttaaatg actcatggaa ggaagtaagt ggttcaaaag acggttatcc tcaatctact
     6661 ttaattgtaa agtctgaatt tttaagagat aataaagatt ttgttgattc ttttgtaggt
     6721 caattatcaa atagtataga ttgggcaaac aaaaatcctg aagaattagg tgcatattct
     6781 gaaaaaataa aaatatctac tgaatcaaag ataataggaa aatctcttga aagagctaat
     6841 ttgaaatata ttccagttaa ggacatgatt aaagattata aaaattacta tgagaaatta
     6901 gctaattttg atgataagac attaggaggc aaagtaccag atgaagcaat atactttgtt
     6961 gagaaataaa aaaaataaag aacaatttat agataaaatg gaggtaacac tttcttgtgt
     7021 tatcctctta tttttatggc aaattattgc tttaaaaatt gataatgata tttttttacc
     7081 aacagtaggt caagtattta atagtataaa agagattatg ctaaattcta gtttctatat
     7141 agatatactt tactctatgg ggagatgttt ttttagtttt ttactggcta tgatttttgc
     7201 tattatactt tcaatagtgt cttatttaaa tagatttttt agaaacttat taaagccaat
     7261 caatgctttg acacgttcaa taccaactat gatattggta gtacttgcac ttatttggtt
     7321 tgaaaaggat agtacccctt ttatagttgg atttactata gtttttccta tattgtatga
     7381 taacgttttg ggcgctatat tgaatataga caagaactta ttagaaatgg ctaatgtgta
     7441 taaaataagg tttatagata aggtgttaaa aatttattta ccagccataa aatttcaaat
     7501 tatatctata ctagtatcaa cattctcttt aagtttgaaa gttgctatag caggagaggt
     7561 atatagtcaa cctacatatg gtattggaac tatgatacag actgaaaaaa ttaactttaa
     7621 cacatctgga atatttgcat ggataattat tgttctttta atatctgcta ttttacaaat
     7681 agctcaaaag tttttagcaa ggagagcttt tttatggaaa agataaagtt gagtattgaa
     7741 aatataaata aaagatatga tagtcgtatt atatttagag attttaatat tgatttttat
     7801 gtaaatgagg taaattgtat ccttggaaaa tctggttgtg gaaaaactac actcttgaac
     7861 ataataagtg ggattattaa aaatgacacc aacaatctaa atatcaaaga aaatttaaat
     7921 agagtaggaa ataagcttga agctagttac atatttcaag atgatagact tatagactgg
     7981 ttaacagtag aagaaaatat aaagatagtt gtaaataaat actataataa aactcaatta
     8041 aataaaattt gtgatgaata cttggaattg gttggaatta gtgattataa aaaattctat
     8101 cctcagatgc ttagtggagg tattagacaa agagtaaata ttgctagagc ttttatatat
     8161 ccatcaaaaa atattataat ggatgaacct tttaaatcta ttgatgcaaa gaatacacag
     8221 cttataatgg ataatttcag aaatatatta agaaaagaaa aaagaactgt gttatttgta
     8281 actcataata ttgaggaagc attgttttta gcagatagga tatttatttt aggagactct
     8341 ccaattagaa taaaaaaaat attaaaaaat tctaaggaat tggaaaaaaa tgaagttttg
     8401 aagttgatat agagttataa taatactgtc gttttaaaat atatttggtt aaaatctata
     8461 agactatatt aaaatagtac agaatattca aaactgtgta taatgttcgt attttataaa
     8521 aaggaagtga atgtatggtt aagactaatg ctatgagaat attagattca aataagattg
     8581 attataaagt aatgtcttat gaagtgaaaa gtgaacatgt ggatggtgtt gaggtggcac
     8641 atgatatagg aagagatgta aatgaagtgt ataagacatt agtaactcaa ggagttagta
     8701 agaatatata tgtatatgta ataccagtac atgaaaactt agacttaaaa aaggctgcaa
     8761 aagtagctaa agagaaaagt gtagagatga ttcatgtaaa agatataaat aagcttacag
     8821 gttatattag aggcggatgt tctcctatag gtatgaaaaa gctttataaa acttttgtaa
     8881 atgaaagtgc aaagaattta gatacaatta ttgtgagtgc aggaaaaata ggatatcaaa
     8941 tagaactatc tccatttgat ttacaaagac ttataaaagt agaatttgta gatgtaataa
     9001 aaaaataaga attacatata aaatatagag cttatattct caataatagg tgttgtagta
     9061 ttaataaaat gaataataat tttaaaataa atattcagtt acttatgtgt aattggtata
     9121 aaaatattga gaaattttaa aaacaatggt aaagtgtaag tataatcacg aatatttttt
     9181 ataaagtaat ggagggatgt tcgcttgaga aaaataaaat ctgaacaaat cgtcgagcag
     9241 gtaaaaaaac tttgtataga agcgagccta tatttaggag aagatgtttt aagctgtata
     9301 aaagaaaaag cgaaaagtga aaagagtgaa gttggaaaaa atatattgaa tatattagtt
     9361 gaaaatgctg aaatagctaa agaaaaaaat ataccaatat gccaagatac tggtatggct
     9421 gtattttttg ttgaaatagg acaagaagtt ttaattgaag gagacacact tactgatgct
     9481 ataaatgagg gagtaagaca agggtatgaa gaaggctact taagaaaatc tgttgtaagt
     9541 ccaattaaca gagtaaatac aaaagataat acacctgctg ttattcatta tgatatggta
     9601 aaaggtgata aaattaaaat agaatttgca gctaagggat ttggaagcga aaatatgagt
     9661 aagatgaaaa tgttaaagcc atcagatgga ttggaaggaa ttaaaaaatt cataatagat
     9721 acagtttcag aagctggacc taacccatgc cctcctatgg taattggagt tggtataggt
     9781 gggactgtgg acaaatgtgc tcaaatagct aaaaaggcac tttttagaga actaggtgaa
     9841 tttaataagg acgaaaatat agcaaaactt gaaagtgaac tattaacagc tattaataaa
     9901 cttggtatag gacctcaagg cttaggtggt actacaacag ctttgggatt aaacatagag
     9961 acttttccta cacacatagc aggtttacct gttgttgtaa atataaactg ccatgcatca
    10021 agacataaga aggtagtaat ttaggaggaa aataaaatga taaaaataac tacacctgta
    10081 aatgaaattg atatagcaaa attaaattgt ggagatacaa ttagtttgag tggtatactt
    10141 tatacagcaa gagatgctgc tcataaaaga ttgatagatt gtataaataa aggtgaagaa
    10201 ttaccatttg acgtatatgg tcaaggaata tattatgtag gacctactcc tacaaagcca
    10261 ggagaagtta taggtgctgc aggtccaacg acgagttata gaatggatga tttgactata
    10321 cctcttttag aaagaggtct tagattgatg ataggcaagg gaaaaagaag tgatgaagtt
    10381 atagaaggta tgcaaaaata cggagcagtt tatcttgctg caataggagg agctggagcg
    10441 tatatttcaa attcaataaa atcatgtgaa attattgcat atgaagattt aggagctgaa
    10501 gctataagaa agattgtagt agaagacctt aagcttacag tagcaataga tagctatgga
    10561 aataatatat atgagcaagg tcgagcaatt tatgaatgta aataatgttt agaaataaat
    10621 atgaatatca aaattaaaaa tagttgtgta tattttaagg aggaaaattg atgtctaagg
    10681 attatgcaaa attggcatta gaaatgcatg aaatcaacaa agggaaagta agtgtagaaa
    10741 gcaaggtaga aattaaaact aaggatgatt tatcaacagc atatacacca ggggttgctg
    10801 aaccttgttt aaaaatacat gaaaatcaag atgatgttta tagatacact tctaaaggga
    10861 atctagtagc agtagttagt gatggaagtg ctgtacttgg attaggtaac attggtgctg
    10921 aagcatctat acctgttatg gaaggtaaag ctatattatt taaacaattt gcagatgtag
    10981 acgcttttcc aatatgttta aagactaatg atgtagatga gatagttaaa tctgtagagt
    11041 tgatggagcc tgtatttgga ggtataaatt tggaagacat atcttctcca agatgttttg
    11101 agatagaaga gagattaaaa aaatctttaa gtatacctgt ttttcatgat gaccaacatg
    11161 ggacagcaat aatagttgca gcagccataa taaactcaat aaagttaatt gaaaataaaa
    11221 aaatagaaga tttagaaata gttataaacg gagcaggagc tgctggtata gccatagcaa
    11281 agatactact taatatgaat gttaaaaata taattttatg tgatagaagt ggagcattgg
    11341 aagctagtat agaaaatcta aactatgttc aaaaagaaat gttaaaagta acaaatataa
    11401 gaaatgaaaa aggaccttta aaagatatta taaaaggaaa agatgtattt attggtgttt
    11461 caggtccagg agctgttaca aaggaaatgg ttgaaagtat gtctgagaag ccaataatat
    11521 tggcaatggc aaatcctact ccagagataa tgccagaaga agcaaaatta ggtggagcat
    11581 ttataatggg aacaggtcgt tcagacttcc ctaatcaagt caataatgta ctggcttttc
    11641 ctggaatatt tagaggtgca cttgatgtta aggctaaaga gataaatgaa gaaatgaaga
    11701 tagctgctgc atatgcaatt gctaatacaa taagtgatga agaaatatgc ccagagtata
    11761 tattaccaga tgtatttaat aagaatgtag taaaaaatgt tgctaaggca gttaaagaag
    11821 cagcgataaa aactaaagta aatagaatat aaatttttat atgttcatat atccttatat
    11881 tatcgggtat atgggcattt ttattatagg tttatactca tatatcctta cttttatata
    11941 catatcttta tgattaaagg tgttagaaaa tgtagtaaat tttatactat cagactctat
    12001 ctttattact ttagtagata catggttgta aaggtttata gaaaaaaata taggaaggca
    12061 gatttttatt gttgcgtata tatgagaatg atataatatg aatattataa attaataaat
    12121 ttaaatttat ggaggtacaa gagatgatgg aatataaagt taatgaccaa gaacttaatg
    12181 cttcaatgtt tatcccattc gtcaacagga tatggccagg agattatgat atggataaaa
    12241 cacagactgc attatcaaaa actttgaata taactgctta tgatgataaa gtgcttgtag
    12301 gatgtctgcg agttctttca gatggttatt attttggaac aattacagaa ttgcttgttc
    12361 ttccagagta tcagaaacaa ggcattggta gtaagctttt ccagcttgct aaagagaaca
    12421 ccccaactat gctatatttt ggtgcacagc caggagtaga agagttctat gaaaaaaatg
    12481 ggtgccaaag aagtctgcaa tcctacataa taaaaaagaa agatagttat taaagttcca
    12541 gctttcaata atcttaaaag tacaatattt tagtggaatc atttcatttt ttattactaa
    12601 tatgacataa acatgtcata ttagtattga tatactttta atatggttaa gagccaaaga
    12661 aaaatataag gagtgattta aaatggaaga aactaaattt tgccaatgct gtgcaatgcc
    12721 aatgggacaa actgatgaat tatatgggac aaataaagat ggaagtaaaa gtactgatta
    12781 ttgtagttat tgttttaaag atggtgagtt tacagctgat ataagtatgg aagagatgat
    12841 agaagtttgt atccctcata tgcttcaaag taataaggat atgactgaag atgaagcaag
    12901 aacaatgatg aacaatttct ttccaacatt aaaacgctgg aaataatttt atgcaaaatt
    12961 agttatatgc aaaacatgaa atatggtgtt gagaataagg ggtggtgtgc tttatagcat
    13021 accacctgtt ttgttacttg gagttcagtt attttcctaa atattttaaa tatactattt
    13081 tagataagtt ccctcttcat cttgataaat taatcctttt ttcataagca ctccaagagc
    13141 tcgtttaaaa tttttcttac ttgaattaaa taatatagaa atatctttag ggtcagattt
    13201 atcattaaat ctcatatatc catcagaacc ttctaaataa cttaaaatct tattttctaa
    13261 agtatcaagt tcatcctttc tatttcctct aggagaaaga ccaagttttc catcttcata
    13321 tatctttatg acatgtaaat tttcaagaac atcaccagtt tttagttcag taaagtactc
    13381 attatgaagt attacaccag catatttatt gtctacacat atcattgcag aattattagt
    13441 ttggaatcca tatacaactc cagttacaat atctcctaca ttgtaagtat ggtctgttaa
    13501 taagtatgaa tctatatctg tagtagcagc aattcttttg cttttgtcta aatatatata
    13561 aaataaatat ttttcgtctc taaatagtgg ataagtctta gctttaaaag gaactaatat
    13621 gtctttagga agtcccatat ctataaaagt accaatgtct gtattatcaa ctacttttaa
    13681 atgtgctaca tccccaacct tagctaaagg cggtattaaa gttgcagcag ttctgtcatc
    13741 agaatcttta aatatgaaag catttacttc atcacctatg tttatttcat tttttccgat
    13801 taatctatta tgaagaagta tatcgtcttt tgtgttatta gtttgtccat ccaaaaaata
    13861 tccaaattca gtttttcttt ttactgtaag tttattaaaa tcacctatgt ttatcaaatt
    13921 aaaatcatcc tctcatttta tatttataat ttagccataa tatacttatg tagtagtttg
    13981 tcacagtatc tatattagta ttagtgaaat ttacaataaa taatatagtt atttatgtat
    14041 ttttcaattc tctagcttct tatatagtat aaactccaat ttcttttttg tctttaggtt
    14101 ttattaaaat ttttacaaaa taagttaaaa agtattgaaa taaaatttct tttaaagtat
    14161 aattggtata taccaaaatt ggtatatacc atatcgaaaa gtggtgacta tatgaaaata
    14221 gtaaataaat ctagtaatat accattgcat actcaattaa gttcaataat aagagagatg
    14281 atagaaactg gtgagcttaa agagggcgat gcaataatgc cagaaagaga actttgtaac
    14341 atacaaaatg taagtcgtat gacagtaaat aaaaccattg ttggattagt tacagaagga
    14401 cttctttata gggtacaagg taaagggaca ttcgttgcaa agcaaaaaaa gaagtatcag
    14461 ttttcaaatg taaaaggttt tactgatgtt atgaaagaaa agggagtaaa cataaaaacg
    14521 gatatattat cctttgagat ggaactacca gatgatttag ttaaaagaaa gcttggaata
    14581 agtgataata ctacaaatat atataagatt gtcagactta gatatactga tggagagcca
    14641 tttggattag aaatagtata tctatcagaa gaaatgtgta agggtctaac aaaagggata
    14701 ttggataatt cttcattata tagggtttta aatgaaaaat atggctataa gatacaaaaa
    14761 gctgaacagg taatggagcc agtaatactt tcagatgaag aaagtaagct tcttgaaaca
    14821 gatgaaggtg ctttagctct taaacttcat agaaattcct ataatagaga aggtagccct
    14881 atagaatata ccatatcaat atttagaaca gataaatatc aatatgaaat agtattgagt
    14941 gagtaggtga ataatatgaa atgtttaatt aatggaaaga taatattaaa aaatcaaatc
    15001 ttagaaaata aagtattagt atttgatgaa aaaataattg atatagctga tagtgtacca
    15061 aaagattgtg aagttataga tgctgatgga aaatatatat ctccagggct tattgatatt
    15121 catatacatg gaaacatggg aaaagataca atggattcga ctgatgaatc tattgaaaca
    15181 atttcaaagt caattatgag acatggagta acatcttttc ttccaactac aatgactatg
    15241 gataaagaac atgtttatga tgcacttgaa gttattaaaa aagcccaaaa tagaaagctt
    15301 gaaggggcac aagtattagg agcacattta gaaggacctt ttataaatga gaattataag
    15361 ggagcacaaa atgaaaaatt tataataaat tcaaaatacg aatttataaa agaatataaa
    15421 gatgttataa aagttataac ttatgctcca gaaaaagata ttgattttga ttttactaga
    15481 gaaataaaaa gatgtacaga tatagtttta tctattggtc atagtaatgc aaattatgac
    15541 caagctaaag aagctataaa tttaggagta actaatgtaa ctcacatgtt caatgcaatg
    15601 actggtttaa accatagaga cccaggcgtt gttggtgcag cacttacaac taatgtgtac
    15661 agtgagttaa tagcagatac aatccatata aataaagatt tatttcaatt tatactaaat
    15721 aataaaggaa aagaaagact gattttaata acagattcta tagaagctgg tggtcttgaa
    15781 gatggcaatt attctcttgg aggtcagaaa gtcatagtta agggtaatga agctagactt
    15841 gaaaatggag cattagctgg aagtgtgttg tctttaaata aaatggtatt taacttctta
    15901 gataatacaa accttaaagt gaatgaagca attaatttag cttctttaaa tccagcaaca
    15961 tctttgggaa taaatgacaa aaaaggaagc ttagaaatag gtaaggatgc agatatagct
    16021 gtatttgatg agaatttaga ttgtaaaatg acactttgct tgggggaagt agtatataaa
    16081 aatatatagt atacaaaaca aactttaaag acagtaaaaa caatataaaa aataataaat
    16141 taaacttaca ttagttaaag tagtagttaa aatttaggag gaataagatg agagtattag
    16201 tgtgtaaaga ttatgatgga atgagtaaaa aagccgctga aatgatagca gctcaaatag
    16261 ttttaaaacc aaattcaata ctgggactag ctacaggaag tacaccagta ggtatgtata
    16321 gagatttagt aaaaaaatac aatgataata tagtagattt ttcagatgtg atgagcttta
    16381 atttagatga atattataag ttaccaatat ctaatgacca aagctatgat tattttatga
    16441 aagagaatct atttaatcat gtaaacataa agccagaaaa tactcatcta ccaaatggaa
    16501 tggctgatga tattgaaaaa gagtgtatga actatgaagc ttctatagat gcagctggtg
    16561 gtatagatgt acaagtactt ggaataggtc gtaatgctca tataggtttt aatgaacctg
    16621 atactaaatt tgcaaagaga actcatgtag ttgaacttac agagtctaca atagaagcta
    16681 atgcaagatt ctttaaatca agagaagatg ttcctaaaaa agctgtaagt atgggaatag
    16741 gttcaatatt gaagtctaag aaaatattgc ttttagcttc aggagaagaa aaagctgatg
    16801 cagtatataa tactgtttat ggagatataa ctccagaagt tcctggttct atacttcaac
    16861 ttcataaaga cactatagtt atagtagatg aagcagcagc aagtaaattg aatccaaaag
    16921 attataaatt agtataagaa tagaatgtag tttaagtaaa aaatagctat cattgaataa
    16981 tcaaatttat ttaatgatag ctatttttat ttaaattttt tacttctttt tatttaaatt
    17041 agttattatt gtttaaatta cttgcttatt actttttacg aaaactcgtt atcaagaagt
    17101 atccaaataa gatgttaaaa attattatgt ttagtgggat tctatcaagc ataataaaat
    17161 tacctataat tgggatatta ccaactggaa acataaagaa gaatacaggc atcagtgata
    17221 gtaaaaaaga tggaaaacaa agaagaagta atcttggaat atcaatatac caactaccat
    17281 cctttttgta ttctctaatc aataattcag ttcctagtaa taaaccaata ccaccagagg
    17341 aaattaaatt aataaaaatg tatcttttaa agtcttgcat ataaccagtg taaaattgag
    17401 tatagaaatc ataatttaat ttaacagcta aaaaatacat aagcaataga atgattaaaa
    17461 ataaaagata tctaagagct tttttcattt taaattctcc cttcagaaat actttattca
    17521 gttttatttt gttggaatct gattttatca tttagtttaa gaaatccata atattagtga
    17581 agaatgaaat aagtattata tatgaataaa tatttttaga attaaggtga attgtagtgc
    17641 ttaatataac tataatatta attgtttgaa tattcaatga aaagtttgaa aaatatcaat
    17701 tttgatataa actgaataaa tacagttgaa tattaaatta ttattttatc aaaaatatgc
    17761 aaagtactac accttcaaat atcatctaaa gtaattaaaa ggcaattaag gtgaagtagt
    17821 tcaagtattt ctaaaataag tttaataaat tctataataa tttatttttt gccttaaaat
    17881 tgccttaaaa tccaaacaat tttaaaacta atgtactata aaaagctggt ataatttaaa
    17941 tatagtaaaa aataagattt atcttatcta ttgggattta attatttaat caatatattt
    18001 ataccattta atttaaataa aaagtctatt aaggagtgag cttatgagta agttaattta
    18061 tatagcagat gatgaagata atataagaaa tttggtaaag acattcttaa aaaatgaagg
    18121 tcatgatgtt atggatttta aaacgggtga tgaactttta gaacaattta atataaagga
    18181 gtgtgactta gtaatactag atataatgat gcctggctca agtggttttg aagtatgtac
    18241 aaaacttcgt gagaaaagta cagtcccaat aatcatgctt actgcaagag atacagatat
    18301 agattatatt acagggataa ccttaggtag tgatgattat tttacaaaac catttagccc
    18361 aatgtctttg gttatgagag taaaatctat atttagaagg atagagtttg aaaagaaaca
    18421 aaattatgat aaatatagta actcgataga tatggaatta aaatttggtg atgtaataat
    18481 aaataaaaag aataaaatcg ttacaagtaa aaatgtaaat atagacctta caccaaatga
    18541 atataaccta ttgacttatt tgtttgaaaa tatagataga gcagtttcca gagatgaatt
    18601 attaaacaaa atatggggat acgatataga agttgagaca agggctgctg atgatacagt
    18661 gaagagatta cgtaaaaaaa ttttaaatac aaatatttta atagaaactg tatgggggtt
    18721 tggatttagg ttgaaggaga aaagttaaga tgaagaatgg aaatgacaag aatagaattg
    18781 gaaagattaa atccaaattt aaattaaagt tctcaaagaa aaatataagc ataaaaacta
    18841 ggattttgtt gagtatactt tgtttgataa ttatagtatt tatgataata ttagtatctt
    18901 ttaatgtatt tcttgataca tatataaaaa caactgcgaa tgaagaattg acaaagtcta
    18961 cagaaatagt agaacatatg gatagtaatt ttagacctat taagccacca caagatgatg
    19021 gaaagcttcc tcccaaagta ttatctaatt ttatgagaaa tgtacaagat aaagttagga
    19081 tggcagaaac acaatcagat gctgatgcta tggtagttga ttctaagtat aacctgatat
    19141 ttccaacaag agaggatgat ttcttaaaaa atgttgatga gatggagctg attagggagt
    19201 gtattcaaaa tgaaaaatta gatttaaatt cagatgcaaa tacacggata tctaccagaa
    19261 ataaggatta ttatatttca actgtaaaaa ttactagtat atatggtgaa caagacaagt
    19321 atcttatact ttttatagac atatcaaaaa ctctaaatct agcacaaaag ataaatattg
    19381 tattaatatc tgtaatgtgt tttgctggaa tattagcaat atttacagct gtgattttat
    19441 ctgagaaaat tgctaaaccg ataaaagagt tgtgtgagtt tgcaaaaaaa atgggtcaag
    19501 gagatttcaa aagaacctac tttgactttt cagataaaga attagtagag ctctcaactg
    19561 ttatgaataa gagtgctgag tatctggata agtatgataa tgaacaaaaa atattttttc
    19621 aaaatgcatc tcatgaatta aggacaccac ttatgtctat taaaggttat gcagaagcta
    19681 taaaatataa tgttatagat agtaaacatg caagtgatat aattcttgaa gaaagtgata
    19741 ggttaagtga tatggttgaa gatttattat atatttctaa aatagataat ataactaaag
    19801 attttgaact tgtagaatgt gatttaagag aagtactttc aaattgtgga gctaggcaaa
    19861 atgtaagagc tataaataag gggataaaat ttatttatga atttgatgaa gaaatggttt
    19921 tatttgaatg tgatgaaaaa aacatttcca aagcatttat gaatcttata gaaaatgctt
    19981 tgagatacgc taagagtgaa ataaaaatag tgtgtaagta taatcaaaaa aatatagttg
    20041 tgataataga agatgatgga attggaatca agaaagaaga tttacctcat gtttttgaaa
    20101 gattctataa gggagtagga ggaaatcatg gaattggatt atcaatagtt aaatctattg
    20161 taaataagca tggtgggcgt atatacgtag aaaatggcaa aaaaggagct aaatttacta
    20221 tattatttaa attgtaaaaa aattagagaa aatatagaga tgtttgaagt tgttttttat
    20281 atatatctgt tgtatactgt tgaaaaaaga acaatattaa gctgacatat attacgatag
    20341 ttgacattta aagtaaatgc ttaaatatag atacgaaagg aaaagtaaaa tctgaagtgt
    20401 atttaggttt aagtaagctt cttattttat aaaaaatatg tatgaaagaa cagctgattt
    20461 ttaatattac aaatttagta acggttgtat ttgaagcttt tgtcatccat atgtttttat
    20521 cagacttttt aggccagaaa gaagggtata taaaagttgt aagatatgct aaactgggtt
    20581 ttataatatg tttaggattc tgtaatttaa taactttaaa ccctaaaatc acaatgcctt
    20641 taattttcat ccttattttt tcaacatcat ttttatataa aggaaattta aagacacgat
    20701 tgtttactac tgttttatta agtatattct ttatactgtc agaaattgta gttacaagta
    20761 tttttgtttt atttgtaaaa gaaggctttg aaataatgct tgaaaataat agtataagag
    20821 tattggcgac aattttatct aaaattgtct ttttgttgac ttgtaaaatt atttgtctat
    20881 ttaaaaaaga tgtacattta gatatgccca taaagtattg gctcccttta tttttaatac
    20941 ctatatttag tttatttctg tctgtttcta tctttgatgt aagtaaattt tttagtcttg
    21001 aaagtttaaa atttctaagc ttaatatcta gtgttggtat tttatatatt aattttattg
    21061 tcttttattt atttaaattt attatagata aaactaaact atctatgaaa tatgaactac
    21121 ttgaaaaaga aataattcat aaggaagaat taagacttag taatgagtgt tataaaatta
    21181 ttgttgagca gactgattct gtcgttttcg aatggaatat caaagaaaat aaatcatttg
    21241 tatcgcaggc ttggactgaa aaatttggat ataacaatgc atgtaagaat atttttaaag
    21301 aaattaaaga taaagactta gttcattctg aagataaagc tatatttgaa ggttttcttg
    21361 aaagtattaa aaaaaagaat atgcacaatc aagcagtata tcgtttaaaa aaatctaatg
    21421 gtgaatatat ttggtgtaga acatcaatta caagtattta taatgatgaa aatgaacttt
    21481 taagagttgt aggagtaatt gtagatgtag atagtgatat aaaaaaatat gaagagttaa
    21541 gaactagagc agaaagtgac tcattgacca atatatacaa taaaggtaca tttgaaaaat
    21601 tagtggaaga aactatagtt atgaatacag gtgataaaaa agatgcatta ttcattattg
    21661 atttggatga ttttaaagaa ataaatgata attttggtca tccatttggt gattttgtac
    21721 taaaaacatt tgctgataag atacaaacta gttttggtag taaagactta gtcggaagaa
    21781 ttggtggaga tgaatttgtt gtatatatgc aagattatgt tacagaagta aatttgcata
    21841 aaaaagctaa agaattaaat agagtacttt cggataatta cactgatttg agttttagtt
    21901 ttgatgcttc tgtcagtata ggtattgcaa ggtatcctca agatggaact tcattttttg
    21961 aattgtttaa aaatgcagat agagcacttt atagcataaa agcatcaggt aaaaattcat
    22021 attgtttatt tgaagaagaa ctatatgtac agtgaatttg aatttagatt ttatgtgaga
    22081 gtaagattta tatatacttg tggctgtctg atttataaaa tatgttactt tgtatatttt
    22141 tatatgtttc agtaaggtta attaacataa gcaaaggtgt aagtaaattt atattagttt
    22201 aagtttactt acacctttat aaaaatacaa ataatagatt catttacatt cttaaatgat
    22261 taaagctctt taccttctaa acatgcataa catatcgtta taattccagt aaataagact
    22321 aaaataccca ttcctaagat tacagtttca ttatctatta ctccaaatgt ttttaaagta
    22381 acttatttcg ctttgtatca taataatttg tctcttttta cattttaact tctgctgaga
    22441 tttgagttag atattcatta gaatctcgca cacttatatc atatagaaga tattcttcat
    22501 aaacaaaatc cccaatacgt aaattattct gattaaaaaa atttaataac ttttcataag
    22561 tcttgtaaat agtttcatag ctccctttat gataagcagt tacataaagt ccctttggtc
    22621 tgattgatac aggatatttc ttgtactctt tattaacttt tgtaaataaa tatgaatatg
    22681 attcaaagtc tttattaatt atatcttcac ctttaatgat tgagccaatc gtaccataat
    22741 cactcaattc atagttgtcc ataaagttaa tacattcata taaaaaattt tcttcctcat
    22801 ctcctaaaaa ttgttcttta tatgtaacag gtgttttgac caagtactct tcttcatgtt
    22861 cttttaaagt aatagtattc gcatctatat tacaggcatt tttagtgaat gaaatagtag
    22921 attccattag agcttgaata ttatttagtt tttcaatttc atttttaata tcaatggttt
    22981 tcttttctaa aagctcaatt agtaaattag gtgttctttt gtctatataa gctttaattt
    23041 cttttaaagg catttttact tccctaagtg aagaaattat accaaaaatg ctaagttgat
    23101 ggtatgagta gtatctatat ccattttctt tctttaattc tggtgagaaa agacctatat
    23161 catcataatg gaaaagtgtt tttttattga ttccacaaat ctttgcaaat tctccagttg
    23221 taaaataatt ttttgaaact tgactcataa ttataattcc tccttgacta tcctgttaca
    23281 gtatagttta tcatagtatt ttgtaaagga gtgtgattat atgaaaaaat taattcattt
    23341 tttaaaacca tatagagttt taattgttgt agttttgata tttacgtttt tacaaacact
    23401 tggaactttg tatataccaa cacttacagc aaatatagta aacaatggtg tagtaaaggg
    23461 agatatagat tatattgtaa aaacaggttt aatgatgatg atagtagctg gtataacagc
    23521 tttatcagct gtgttagttt gtaaagtatc agcaaatctt tcatctggat tttgtagaga
    23581 tattagagaa gcagtattta taaaatcaca agatttatca ataaatgatt ttaataatat
    23641 aggtacagca tcaatgatta ctaggagtac tagtgatatt actttaatag gtcagtctgt
    23701 gtttatgttt attcaattag tattaccagc accaattata actgtttctg gtctattctt
    23761 agcatattct atagataaag caatgacaat tattattgtt gtagtaatgt ttttattcat
    23821 gctttcagca tttttagttg gtaaaaagct tataaagttg tttaaaatga tgcaaataaa
    23881 gatggacaat atgaataggg tgttacgtga ggtagtaact ggagtaagag ttataagagc
    23941 ttttaatagg agtcgttttg aaaaaaagag atttgataga actgcaatag attattctga
    24001 aacagcaatt tcaataaata aaatatttgc agtacttatg cctattgtaa tgttaataat
    24061 gaatttaggt atagtaagta ttatttggtt tggtgggatg agggtcagta atggaaatat
    24121 ggaaataggt catataatgg cacttgttga atattgtatc ttaattttat tctatttaat
    24181 catgggagta atggtattta tgtacatacc tagagcaggt gcatgtgcag atagagtaaa
    24241 tcagatatta gatatagaac ctgaaattgt agatggaaat ggtcataaag atactgtctc
    24301 agagagagga catttagtat ttaaaaatgt aaccttttca tatgcacaat cagaagaacc
    24361 tgtgttaaat aatataacat ttgaagcaaa atctggagaa gtaactgcaa ttataggaag
    24421 cactggctca ggcaaatcta ctattgcaaa tataatacca cgtttttttg aaattcaatc
    24481 tggagaaata tctataaatg gtcaggatat taagaagatt cctcaaaaag aacttagaga
    24541 taagattgga tttgtaccac aaaaagcttt tttatttagt ggaacaatag aagaaaatat
    24601 aaggtatgga aaagaagatg caagtattga agaagtaaag catgctgcaa gtatagctca
    24661 agctgatgaa tttatttctg atatggaaga caagtatgac tcatttgtag cacaaggtgg
    24721 aaataatttg tcaggtggtc aaaaacagag aatatccatt gcaagagcgt tagttagaaa
    24781 gcctgaagta tatgtctttg atgatagttt ttctgcatta gattttaaaa cagataaaag
    24841 acttagaaaa gcgttgaaaa atgaaatcaa agattcatca gctataatta tagcacaacg
    24901 tataagtaca atcatggatg ctaatcagat tatagtttta aatgatggaa aaattgtagg
    24961 tattggaaaa cataaagatt tacttgaaaa ttgtgaagtt tataagcaaa ttgcggattc
    25021 acaacttagt aaagaagaac ttgcataaaa tgaagaatta atctgatatt tggaggtgta
    25081 tgatatgagt gaaaaaagaa tagatgaaca agatatagaa gtacttagtt ttgaagatgg
    25141 catgtcagca gaaaaagcta aggatgcaaa aaaaactact aagagattat taaaatatat
    25201 ggcaaaacaa aagtttaaaa tattaattat ttttatttca gtattgataa gttcagcgct
    25261 tacagtttta gcacctatgg ttatgggaaa agctattgac caactattta atggaataaa
    25321 aactgcagtc caaacaggta caaaattcag tgtgaatttt agtactatgg gagggatagt
    25381 atcaatactt ctagggttgt acttaattag tgcagtattt atatatattc aacagtatat
    25441 tatgtcagga gttgcacaaa atctagttct tagcatgaga aaagatttaa gtgataaact
    25501 caataaactt cctcttaagt actatgattc tcataaaaag ggggaaacac ttagtatagt
    25561 aacaaatgat ttagaaaaag tagcagatag tttacaagaa ggattaatgc aacttattac
    25621 agcagtagtt acagtagttg gttctattgt aatgatgata agtataagtg taccacttac
    25681 aatagtatca gctataacat tacttgtttc attgggtatt actgttgtaa ttgcaagaaa
    25741 atcacaagaa agattctctg aaaatcaaaa ggcactagga gagttaaata gcaatataga
    25801 agagatattt actggtcaaa tagtggttaa agcatttagt aaagaaaagg atactataag
    25861 aaactttaaa atattaaatc aaaatcttta taatgcaagt agaaaagcac agttttcgtc
    25921 atatgcaata tctccaatta ttagatttat aaacctaatt ggctatgtta ttatagcagt
    25981 tgtaggtggg atatttgcat caactggagc aatgactctt ggtagtatac aagcgtttat
    26041 tcaatatgta aatcaagcat cagagcctac tacagaaata tcttatattg taaatatgct
    26101 tcaagcagca atagcatctg cagagagagt ttttacagtt atggacgagg tggaagagat
    26161 aaaggatgaa gaatcttcaa aggttattag tatgcctaaa ggtaaagtac aatttgagca
    26221 tgttaaattt ggatatagtg atgattttat tcttatgaaa gatattaata tcaatctaaa
    26281 tgcaggagat aagattgcaa ttgttggacc tacaggagct ggaaagacaa ctcttgtaaa
    26341 tttacttatg agattttatg agatacaagg tggaagaatt actattgatg gtgttaatat
    26401 aaaagattta aagcgtggag aacttagaac tatgtttggt atggttcttc aagatacatg
    26461 gctatttaat ggaagtataa aagaaaatat tgcatatagt aagtcagatg ccacaatgga
    26521 tgaaattgta agagcagcaa aatcagcaag agtagaccat tttataagga ctttaccaca
    26581 agggtatgac actatattga ctgaggatgc ttctaatcta tctcaaggtc aaaagcaact
    26641 tctaacaatt gccagggcaa tactttcaga cccatcagta ttgattcttg atgaagcaac
    26701 atctagtgta gatactagaa cagaggtaga aattcaaaag gcaatgaata acttgatgaa
    26761 gggaagaact agttttgtta ttgcacatag attatcaact attcgagatg ctgatttaat
    26821 attagtaatg aaagaaggaa ctattattga acaaggaagc cataaggaac ttattgaaaa
    26881 gaaaggattc tatgaagaac tttataacag tcaatttaca agtgaatatg atgaagatgt
    26941 tgtatgatta tcaagtttta ggcaactaaa tgattgataa atattaagtt aaatatagta
    27001 acatgtaagg ttatatggaa taagagtatt tctctatttc atataacctt ttttatttat
    27061 caaaataaga taaaatttcc tttctaaatt agattgaaga atgtatttat caatataaaa
    27121 gtaaaataat cattattatt agtgaagtaa atattgtttg ccacatatat gccataaaga
    27181 tgcacttatt ttacggagat ttaacaaagt agtgtaaagt atactctaaa tataaaataa
    27241 atcaaataga gttaaataaa ttgagaggtg atataaatag tgaataaaaa attgaactat
    27301 agatttgaaa tgaagcacaa aataactgaa gcagatgtgt tagctttaaa atcaagactc
    27361 tatccaataa tgaagaaaga tgaaaatgca tctaaagatg gaaaatatct tattaggagt
    27421 ttatattttg atactcctga agataaagca cttttggata aattaaatgg cgttgcaata
    27481 agagaaaagt ttaggataag attttataat aatgactgct cttatataag gttagagaaa
    27541 aaaatcaaac attacaacat gacatcaaag ctatcaacta atttaaccaa aaaagaagta
    27601 ataaatatac taaataatga tattgaattt ctaaaagagt cgacaaattg tttattaaga
    27661 gaattttatt tgaaattaaa atgtgaaaga ttagaggcaa aaagcatagt tgattacaat
    27721 agagaagctt ttatttatcc agttggaaat gtaagagtta caatcgatag tgatattaaa
    27781 acttctgtaa attcggtaga tttatttaat aaagatttgc caactgtttc ggtaattgat
    27841 gagaatatga ctgtattgga ggttaaatat gatgaattta taccagattt tataaaggat
    27901 ttaattcaaa ttaataagac tacctctaca gcagtttcaa agtatgcttc aagtagatta
    27961 tatatttaag aataaaggct atgatttata taaagatata cataaataag gttaaaaaac
    28021 aaataagtta aaaataaata aaataaaaaa taaataagtt aaaaatagga aggatgattt
    28081 taattatgac atttaatgat atttttaaat caagttttat agaaaacgta tcaggttttt
    28141 catttgtgga cagtgcttta gcactaggtt cagctttttt agtaggtttg tttatatata
    28201 tggtctataa aaagacttat atgggaatta tgtattctcg accattcaat gtgtcgcttg
    28261 tagcacttac aatgcttaca acttttgtaa tattggcagt tacttcaaat gtagttttat
    28321 ccttaggtat ggttggtgct ttatctatag taagatttag aacagcaatt aaagacccaa
    28381 tggatttagt atttctattt tggtcattgg gttcaggtat agttttagga gcaggtttga
    28441 ttcccttagc tgtcatgggt tcaataatta tgggattgat tctaatattc ttttcaaata
    28501 aaacaatatc tgaaactcca tatatattga tggtaaattg taataatgaa gattcagaag
    28561 atatagcaac tgataaaatt aaaaaagtat ttactaaata tcaaataaaa tcaaaaagtg
    28621 ttacaccaga aaaaggaata gaattagtgt ttgaagtaag aatgaaagat ggagaaacaa
    28681 gtcttataaa tgacttgagt caagtagatg gagttactaa tgcagttctt gtaagttata
    28741 atggtgacta tgtagcatag agaggagtaa tcttttatga aagataaaaa atttaccctt
    28801 cttatctcga ttatgattat atttttatgt gctgtagttg gagtttatag tacatctagc
    28861 aacaaaagtg ttgatttata tagtgatgta tatattgaaa aatattttaa cagagacaag
    28921 gttatggaag ttaatataga gatagatgaa agtgacttga aggatatgaa tgaaaatgct
    28981 ataaaagaag aatttaaggt tgcaaaaata actgtagatg gagatacata tggaaacgta
    29041 ggtataagaa ctaaaggaaa ttcaagcctt atatctgtag caaatagtga tagtgataga
    29101 tacagctata agattaattt tgataagtat aatactagtc aaagtatgga agggcttact
    29161 caattaaatc ttaataactg ttactctgac ccatcttata tgagagagtt tttaacatat
    29221 agtatttgcg aggaaatggg attagcgact ccagaatttg catatgctaa agtctctata
    29281 aatggcgaat atcatggttt gtatttggca gtagaaggat taaaagagtc ttatcttgaa
    29341 aataattttg gtaatgtaac tggagactta tataagtcag atgaaggaag ctcgttgcaa
    29401 tataaaggag atgacccaga aagttactca aacttaatcg ttgaaagtga taaaaagaca
    29461 gctgattggt ctaaaattac aaaactatta aaatctttgg atacaggtga agatattgaa
    29521 aaatatcttg atgtagattc tgtccttaaa aatatagcaa taaatacagc tttattaaac
    29581 cttgatagct atcaagggag ttttgcccat aactattatt tatatgagca agatggagta
    29641 ttttctatgt taccatggga ttttaatatg tcatttggtg gatttagtgg ttttggtgga
    29701 ggtagtcaat ctatagcaat tgatgaacct acgacaggta atttagaaga cagacctctc
    29761 atatcctcgt tattaaaaaa tgagacatac aaaacaaaat accataaata tctggaagag
    29821 atagtaacaa aatacctaga ttcagactat ttagagaata tgacaacaaa attgcatgac
    29881 atgatagcat catatgtaaa agaagaccca acagcatttt atacttatga agaatttgaa
    29941 aaaaatataa catcttcaat tgaagattct agtgataata agggatttgg taataaaggg
    30001 tttgacaaca ataactctaa taacagtgat tctaataata attctaatag tgaaaataag
    30061 cgctctggaa atcaaagtga tgaaaaagaa gttaatgctg aattaacatc aagcgtagtc
    30121 aaagctaata cagataatga aactaaaaat aaaactacaa atgatagtga aagtaagaat
    30181 aatacagata aagataaaag tggaaatgat aataatcaaa agctagaagg tcctatgggt
    30241 aaaggaggta agtcaatacc aggggttttg gaagttgcag aagatatgag taaaactata
    30301 aaatctcaat taagtggaga aacttcttcg acaaagcaaa actctggtga tgaaagttca
    30361 agtggaatta aaggtagtga aaagtttgat gaggatatga gtggtatgcc agaaccaccg
    30421 gagggaatgg atggtaaaat gccaccagga atgggtaata tggataaggg agatatgaat
    30481 ggtaaaaatg gcaatatgaa tatggataga aatcaagata atccaagaga agctggaggt
    30541 tttggcaata gaggaggagg ctctgtgagt aaaacaacaa catacttcaa attaatttta
    30601 ggtggagctt caatgataat aatgtcgatt atgttagttg gtgtatcaag ggtaaagaga
    30661 agaagattta taaagtcaaa ataagattaa aattgaatat aaataatttt aatactgtta
    30721 agtctaaata tttacttagc agtatttttt agaaaataat gtgtttgatt aataat
//
LOCUS       DACXMZ010000018        30664 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.018, whole genome shotgun sequence.
ACCESSION   DACXMZ010000018 DACXMZ010000000
VERSION     DACXMZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.018"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            87..1988
                     /locus_tag="JD016_03295"
     CDS             87..1988
                     /locus_tag="JD016_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAU4998656.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            1991..3034
                     /locus_tag="JD016_03300"
     CDS             1991..3034
                     /locus_tag="JD016_03300"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAU4998657.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3049..3354
                     /locus_tag="JD016_03305"
     CDS             3049..3354
                     /locus_tag="JD016_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAU4998658.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3451..3786
                     /locus_tag="JD016_03310"
     CDS             3451..3786
                     /locus_tag="JD016_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU4998659.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3818..5140
                     /locus_tag="JD016_03315"
     CDS             3818..5140
                     /locus_tag="JD016_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAU4998660.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            5150..6220
                     /locus_tag="JD016_03320"
     CDS             5150..6220
                     /locus_tag="JD016_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAU4998661.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6225..7412
                     /locus_tag="JD016_03325"
     CDS             6225..7412
                     /locus_tag="JD016_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAU4998662.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7609..8481)
                     /locus_tag="JD016_03330"
     CDS             complement(7609..8481)
                     /locus_tag="JD016_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAU4998663.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            8715..8834
                     /locus_tag="JD016_03335"
     CDS             8715..8834
                     /locus_tag="JD016_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998664.1"
                     /translation="MEKPNTKVVNFQEYKDRKNQREVNRDELLKLIKKIVTSK"
     gene            complement(8875..9066)
                     /locus_tag="JD016_03340"
     CDS             complement(8875..9066)
                     /locus_tag="JD016_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998665.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9286..10332
                     /gene="cobT"
                     /locus_tag="JD016_03345"
     CDS             9286..10332
                     /gene="cobT"
                     /locus_tag="JD016_03345"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAU4998666.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10364..10924
                     /gene="cobU"
                     /locus_tag="JD016_03350"
     CDS             10364..10924
                     /gene="cobU"
                     /locus_tag="JD016_03350"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAU4998667.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            10934..11707
                     /gene="cobS"
                     /locus_tag="JD016_03355"
     CDS             10934..11707
                     /gene="cobS"
                     /locus_tag="JD016_03355"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAU4998668.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11727..12341
                     /locus_tag="JD016_03360"
     CDS             11727..12341
                     /locus_tag="JD016_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAU4998669.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      12408..12587
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12702..14216
                     /locus_tag="JD016_03365"
     CDS             12702..14216
                     /locus_tag="JD016_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HAU4998670.1"
                     /translation="MGKKIMLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMAL
                     NSFITKEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSS
                     KYHDYKLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPV
                     IIVGDIDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKV
                     PVIGVVPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVS
                     IRYVDYGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQ
                     MLGKKLKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGK
                     KVKGYEIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEIS
                     FTRAILNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14255..15631
                     /locus_tag="JD016_03370"
     CDS             14255..15631
                     /locus_tag="JD016_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HAU4998671.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYVVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTFLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15632..16603
                     /locus_tag="JD016_03375"
     CDS             15632..16603
                     /locus_tag="JD016_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAU4998672.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            16635..17705
                     /locus_tag="JD016_03380"
     CDS             16635..17705
                     /locus_tag="JD016_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAU4998673.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17707..18594
                     /locus_tag="JD016_03385"
     CDS             17707..18594
                     /locus_tag="JD016_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAU4998674.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKECLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18582..19214
                     /locus_tag="JD016_03390"
     CDS             18582..19214
                     /locus_tag="JD016_03390"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HAU4998675.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19223..20449
                     /locus_tag="JD016_03395"
     CDS             19223..20449
                     /locus_tag="JD016_03395"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HAU4998676.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINIFDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20446..21054
                     /locus_tag="JD016_03400"
     CDS             20446..21054
                     /locus_tag="JD016_03400"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HAU4998677.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            21044..21619
                     /locus_tag="JD016_03405"
     CDS             21044..21619
                     /locus_tag="JD016_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HAU4998678.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21612..22364
                     /locus_tag="JD016_03410"
     CDS             21612..22364
                     /locus_tag="JD016_03410"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HAU4998679.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22348..23469
                     /gene="cbiG"
                     /locus_tag="JD016_03415"
     CDS             22348..23469
                     /gene="cbiG"
                     /locus_tag="JD016_03415"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HAU4998680.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSIIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFGHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23466..24191
                     /gene="cobJ"
                     /locus_tag="JD016_03420"
     CDS             23466..24191
                     /gene="cobJ"
                     /locus_tag="JD016_03420"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HAU4998681.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     DKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24188..24940
                     /gene="cobK"
                     /locus_tag="JD016_03425"
     CDS             24188..24940
                     /gene="cobK"
                     /locus_tag="JD016_03425"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HAU4998682.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNKFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIIAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            24959..26437
                     /locus_tag="JD016_03430"
     CDS             24959..26437
                     /locus_tag="JD016_03430"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HAU4998683.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26467..27372
                     /gene="hemC"
                     /locus_tag="JD016_03435"
     CDS             26467..27372
                     /gene="hemC"
                     /locus_tag="JD016_03435"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAU4998684.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27362..28861
                     /gene="cobA"
                     /locus_tag="JD016_03440"
     CDS             27362..28861
                     /gene="cobA"
                     /locus_tag="JD016_03440"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAU4998685.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELVPILNDNDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28878..29843
                     /gene="hemB"
                     /locus_tag="JD016_03445"
     CDS             28878..29843
                     /gene="hemB"
                     /locus_tag="JD016_03445"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAU4998686.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            29996..30574
                     /locus_tag="JD016_03450"
     CDS             29996..30574
                     /locus_tag="JD016_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HAU4998687.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASIIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNVLVTLDLEELKKFDI"
BASE COUNT        12410 a         3059 c         5682 g         9513 t
ORIGIN      
        1 aaataaaaag aaaaggttgt ttttataaaa aatatttttt ataccacttt aaaaaacaca
       61 ataataacac tgagggaggt ggggatatga gagaacgcat tattcttgaa acattaaaaa
      121 atagtagtgg ttatctaaac atagattatt ttgcagataa gttgggggta tctactcgaa
      181 ctatcagaaa tgaaataaaa aagatagaaa ctataaaaga atgtaatggt tttaaattag
      241 aatataagtc taaattagga tatattctaa atattaaaga ccaagataaa ttcgagcatt
      301 atctaagaaa cttaccctca gatttagttg aaaatccaga acaacggttg gagtctataa
      361 tagtagagct tttagtaaat gaaggttata aaactataga acagttaagt aaaaaatttc
      421 tagtaagttc atcccaaata aaaaatgatt taaaaaagat agatgaaaag ataaaggata
      481 cagaattaaa actagagcga aaggctcatt atggaattaa aatagaaggt tctataaaag
      541 aaattcaaag tatattggtg gatagttatt ttagagggaa tagaaatata acagagtaca
      601 ggaataagtt tatagataat ttaaaattag ctaatattag aagtacaata aaaaatgtac
      661 ttaatgaaca tgatttagaa gcaaatttaa cagaacttga ggaaatatta gcacaaatta
      721 ttatattgta tataagagtt agtatgagag tagtgggaaa tgctagcgat ttaaaattat
      781 atacagaaga tttaattata gataaattat tagataaaat ttttagagat aaaaaatata
      841 accttaatta tgatgaaaag tattatttaa aggagcttat aaaactaaag actaaagata
      901 agaaagccac tataaagaat attgataaga ataaacttca agatattatg ttcgaatttt
      961 ttagagagat agataaaaaa tataactcta attttttaga agataaagag ttttttaact
     1021 tattttactt acatattgca tgtttaatag aacgtataaa gaagaaccac aagataataa
     1081 atcctttttc agttaaaata agtcaacagt atccaactgt ttttaattta tctattcaat
     1141 tttcaaaaat catagaaaat gagtatcata taaaaataag tcaagatgaa atagggttta
     1201 tagctactca cattgctgtt ccttttgaaa agagagaaga agctaatttt aataaaaaat
     1261 ataaaatagc aataatatgc tcttctggtg gaggtagtgc atttttgata aatttaagac
     1321 ttaaagaaat ttttccaaat gctgagataa gaaatttttc attactagaa gaaaaagctg
     1381 taatagaatt tgcaccagat ttgatttttt caataacaaa tcttttattt gaaataaatg
     1441 ctccagttat acttataaat gaaattttag acgaattgga ttatttagat attaaagaaa
     1501 gtgttagatt tgcagaccat ataggaaata tatcaagccc aaaacaatat atattaggtc
     1561 tatttgataa gaatcacttt aggtgtataa aagataaagc taagtataag gatatccttg
     1621 atagtatgtc aaaatgtata gttgacgagg gagcttgttc tccaacctat cccaaagatg
     1681 tatgggaaag agaaagctat ttgagtacta tttatacaaa tggggtggct atacctcatc
     1741 caatcgagat gacaggaaac aaaaatataa tatcagtagc tttgatacaa actgatatta
     1801 tttatgaaaa tagggtgccc aaaattattt tcatgatttc attaataaag ggaaatttag
     1861 aacttcataa gcagatatct aaatatttaa gtaagattat ggcaaataag gatatggtag
     1921 acatgctaaa taagtcacaa tcttatgaag agtttatgta taagttaaaa atctatctgg
     1981 gaggttagac atgaatatag agttttttaa gaggttgtca gaagcagatg ggatagcatc
     2041 taatgaagaa gaggttagac atgttttatt ggaagaacta aaagcttata gtgataaaat
     2101 tatctgtgat gggctaggga gtatcatctt ttctaaaata aaagatgaga gtgcaccaaa
     2161 cgtaatgatt tgtgctcata tggatgaagt tggatttatg gtgagaagta tagacaaact
     2221 tggtatgatt cacttaataa caataggagg agtaaaacct cttgcacagt ttgtacaaaa
     2281 agttagaatt acaacaaaag aagggaaaaa aatccccgct gtcataaatg ctacatataa
     2341 taatggtaag gctgaaaata tatatgcaga tattggagca tatacagaag aagatgtata
     2401 taacctaggg attaatgttg gggatatggt aacatataca acaagctttg aggaatttac
     2461 tttaccagac cgattagtgg gaaaggcttt tgatgatagg ataggatgtt ttgtgatggg
     2521 agaagtttta aaagaactta ggaaagaaaa tctaaattgt aatattcact ttgcggctac
     2581 aagtagtgaa gaggttggaa taaggggagc aaagacatca acacagttaa taaatccaga
     2641 tatagtattt gtaatagatg tagcatgtgc taaaaatgag tttgtgagag actatacaaa
     2701 tcagaaacaa attgataaag gtgtaatgct aatgcataga gataggactc tagtccctag
     2761 tagaaaaatg atagattatg taatggaaat tgctgataaa aataatatac cacttcaaca
     2821 tgatatgttt gaatcaggtg ggactgatgg aggagaagca catctcgtaa atgaagggaa
     2881 accttgtgta gtaacttgtg taccagttag atatggtcat tgtgcatttt caatagtaag
     2941 taataaagat ttggagaaca ttataagatt gtatacacag ttaattttaa attttgatga
     3001 agaacaatat aaacatataa gaaattttat ctaaaggaga ataagaaaat gtcaacagaa
     3061 gtacaaataa tggaacttat atcaactgca ggagagagta aatcgaaagc ttttgaggct
     3121 ttaaaaaaag tgaagtcaaa agattttaaa ggagcaaaat tgttaataga ggaggctcgt
     3181 aaaatagata ttgaagctca caggatacaa acagaattaa taactcaaga tttaaatgaa
     3241 gatgttgaaa gtccagcaat gagtctttta atggtacatg ctcaagacca ctatatgact
     3301 tctcagctag caaaagactt gatagaggct ttgatatatt gtctcgataa gtaattgtaa
     3361 tgtagaaata gattatttat gtaaaaattc ttttaaaagt ttagatgctt ttaagagata
     3421 ataaaaatac aaactttagg aggaattatt atgaaaatat tattatgttg tgcaggtgga
     3481 ttttctacta atatgttaat gcaaaatatg aaaaaggtgg taaaagagag tgctaaactc
     3541 aatgaaaaag attttgagtt tacagctata ccagcagatt caatagaaag tgaagttgat
     3601 gattgggata tagtacttat aggtcctcaa atagctcaca aaatagactt cttaaaagga
     3661 atattagagc caaaaaataa gccttttgca gtaatagata aagatgtata tggaaaaatg
     3721 gatggagcta cagttatgaa gttggcatta ataacttata agaaacatca attagctcaa
     3781 ggataataaa aataatgagg gaaaagggag attagatatg ttttcaaaat ttgaagcatt
     3841 tatgaataga gtatttatgc ctttagctca taaggttgac aatcaacgtc accttggttc
     3901 tataaaggct ggtatggttg ctatgacacc attcacaata ttaggaagta tatttgcaat
     3961 acttccagca cttccaaata tgcttggaga aaataatcca ataagcaaat tcatacttaa
     4021 caattcagca atattggatt taccagtaag tctttccata ggtttaatag gattatatgc
     4081 ttgtatgtgt attgctttta atctggggaa ctactataaa ttatatatac caggttgtgt
     4141 tgcactatca actttttctt ttttattcct agtagcctca tttacaccag atggagcttt
     4201 agaaatagga aatttaggag ctaaagggct atttacaggt atgatagcag ggcttggttc
     4261 tgtggagtta tataattttt gtaataagaa gaatatcaca ataaaaatgc cagaaaatgt
     4321 acctgatttt gtatctaggt cttttgaatt aatacctact actataatag tttgtggaac
     4381 ttttatagtt gctcgtttta tttcattaaa agtatttgga gaattacctc cacagatact
     4441 tactcaattt ttatctccat tagtaggaag tatggataac ccatggtcag ttcttgcact
     4501 taactttgct atatgtacta tattcttttt tggaatacat tcatcagtat ttagtcctat
     4561 aactagacct ataatgatta catttatagc tgaaaacata gcagctatgc aagctggaga
     4621 aacaatacct catttcttta cagcaggagc ttcaagtgca ttctttggat ttacaggatg
     4681 tggaattagt ataggttgtg taattgcatg tatgttatca aaaaatgctt cctataagaa
     4741 gattggacgt gtatcattat tccctgcatt atttgggata aatgaaccaa tattatttgg
     4801 agcaccgata atattaaatc caataatgtt tataccacat gtatttggtg gagctataat
     4861 aggtacacta ccaatgtttt ttatgcactg gggatggtta gcaaagccag tatttgaccc
     4921 tccatatgta ggggtattct tagaaggatt tttaactaat ggagattggc attctatatt
     4981 agcaaatgca ttgcaattag tattagcagt tttattatat tggccattct ttaaaataat
     5041 ggaacgtcaa caaaataata aaaaacaaaa agaagaaact aagaatatat tctctaaaga
     5101 agaagaagac ttattggatg atttaggatt ggatttctaa tattttgtta tgaataatac
     5161 aatggagaga ttagtatcct taatctataa aagaggtgtt gacgctcttt tgattaaagc
     5221 aaaaaatact aaaagatata taggagcttt atcaggtagt ggtgtatatg tgcttgttac
     5281 taaggataaa aattatcaaa ttctagatgg cagatatatt gatgaagcag ataaaaaaac
     5341 gactggtttc ataaaaagga ttgtatctca aggttcttat ataccaacta taattgagtt
     5401 attaaaagaa ttaaatataa aaaaattagc aatagaaaac caagctatga gtatacaaga
     5461 gtatctttca ctaaaaaatg aaggttttga aatcaattta ataacaaatg aattatggaa
     5521 gctaagggct ataaaatcta aagaagaaat tgaacttatt aaaaaggctt gtgagattac
     5581 agatgaagta tttgatgagg ttatttctga aattaaaaaa gatatgactg agctagaagt
     5641 atcagctcta attcagtatc atgcattaaa aaaaggtgca agtggaatgt cttttgaaac
     5701 aatagtggta tcaggagaaa gaggtgcaat gccacatgga agacctacaa acaaaaaatt
     5761 gaaagtgaat gaagctataa caatagattt tggtgttgtt tatcaagggt atcaatcaga
     5821 catgactcgt acaatatcta taggtaaacc tcctaaaatt attaaagaga tatatgatgt
     5881 agtattggaa gcacaattat ctgcaataga gtcaataaag gaaggaacta gggcaagtga
     5941 tgttgacaaa gtggctagaa aaattataga taaacatgga tttggcgaat attttaatca
     6001 tggactgggt catggtatag gtcttggaga tggtgaagtc cccactttaa atccaaatag
     6061 tgaagatata ttagtggaag gaatggtaat gtcttgtgag ccaggtattt atattccaaa
     6121 tgtaggggga gttagaatag aagatgatat tgttataata gatggaaaag gtgttccact
     6181 caataagaca tccaaagagt ttataatatt aggagagtaa aattatgaaa tataatttta
     6241 ataaagtatt aaatcgtaaa gaaggaaatt gtagaaaatg gagcaaccat gtgattaaag
     6301 aaaaatttgg cttaaatgaa gatgcaattc ctatggattt agctgatatc gattttgagt
     6361 gtgctcctgc tataaaagaa tctatcttaa aaagagcatc agtaggagat tatagttata
     6421 cttttatagg agatgacttc tatgatgcta ttataaattg gaataaaaga agatttgatg
     6481 tatatataga aaaggattgg ataaaattaa cttttggtac tgtttcaacc cttcattata
     6541 tagtccaagc atatactaaa gaaggagagg gtgtattgat aaatacacct gcttatgacc
     6601 cttttgcaga ggctgtagta aataataata gaaagttgtg ttgtagctca ttaaaattag
     6661 ataacaatag atattattta gatttagagg atatggaaaa tcaaatgaaa tacgagaata
     6721 taagattgct tatattctgt agtccacaaa atccatcagg tcgtatatgg acaaaagaag
     6781 aactatatca agtgtcagag ttatgtttaa agtataatgt aattttggta agtgatgaaa
     6841 ttcatagaga tataatattt aaagattata gatttgtatc tttgtggaat gctcatccag
     6901 aaatttataa aaatagtata atctgtgtat ctcctaataa aggcttcaac ttaggtggat
     6961 taaaaacatc atatgtatta ataagagatg taaaaattag ggagacttta ttagaaaggc
     7021 taaaaagtaa ttcaataact tctccaaatg tatttgcaat accagcaata gttgcagcat
     7081 ataatgaaag cgaagaatgg ctagatgcta tgactagtta tgtagaggag aattttgaaa
     7141 tagtttatga tttctttgaa actaatatac caaaagctaa agtgatgaag tcagactctt
     7201 cttttttagc ctggatagat gtaagagaag tatttagaaa tgaagaagaa tctaaagaat
     7261 tttttagaca tgcaaactta actatggtag ttggaagtta ttttgtgaaa gatggagatg
     7321 gatttattcg tattaatgtg gggtgtccta gagaaacatt aaatgaggct ctaaatagaa
     7381 tcaaaaaaac gtatataagt atgtattgtt aaatcataaa aaaagtatct atggtttata
     7441 taaaaacaca ttatttagtt atcaaatatt actggaattt tgtattccag taatattttt
     7501 tgattaagga atattattga ggaatattaa ataatattat ttcataccct tatattaact
     7561 tatataaaaa tattttatta ttttgggaac gggttagcta acctttatct agtgtatatt
     7621 tatattgtaa gaagcagtaa aaacttttcc tttactaagt ttattaataa acttcttatc
     7681 tttataaatt ttgttagaat ttatactatc agcaagacca taccaaggtt ctatacataa
     7741 aaaaggagca gtagaattag tttcactata ataaggagtc caaataccaa ctagagggaa
     7801 atcttccata gatatagtta catatttact accatcatta ttacatatag acacttcatc
     7861 tatgttggtg tatatcaaag catcattctt gaatacttct ggatttaact gtaagttttt
     7921 taattttcca ataggtatta tatcatctgt aaaagaacca tttaaattga ttttttctac
     7981 atcattttta cttttaaact caaggtagtg tttagaaaaa tcattttgtt cgtaaaaagg
     8041 aaggttgaat gcaggatgtc cacctataga aaagaatata tctttagaat cagtattttt
     8101 tacaatccat tctacctcaa tactattatc atttaatgta tagtttatga ttagctcaaa
     8161 agaatatgga tacttcttta aagtagattc attatctgtt aatttataag ttacaaatgt
     8221 atctccttta tctacaatct caaagtccat atctctagca aacccatgtt gagtcatgct
     8281 atataaattt tcttctatta ttgtttcatt atcaaaaagt ttacctacta tagggaacaa
     8341 tataggtgat tgtctacccc aatatttttt attaccatcc caaagaatgt ttttgtcgta
     8401 ttttttagaa aatattttag ttagttcagc accaaaactg cttgattcga taattaaatt
     8461 ttcattttgt aatatattca tttaaaatct cctttattat attttatgag tatattatac
     8521 tataaagtat aaagattatt aaattttatt tgatattata tttgaaaggt agattttagt
     8581 atattttgaa aattttgttt tgaaaatttg ggattattta agaaattttg tattataaat
     8641 agaaggaaat agataaattg tatagaataa aatatattaa gataagttaa taatgttaaa
     8701 tgggaagtga gtttatggaa aaaccaaata caaaagttgt taactttcaa gagtataaag
     8761 acagaaaaaa ccaaagagaa gtaaatagag acgaactttt aaagcttata aaaaaaattg
     8821 tgacttcaaa atagggagtt gttaaactcc ctattttaat gggtttcata ataattaaga
     8881 cattctaagt ccatctatat aatctttgaa ttgtgagaaa tctgattcac tacttaattc
     8941 tatggtttct gaaccttccg tataaaaatt cttaagatag atagtataat tattatcaac
     9001 acaatcatga taaatacatc tatatccatc ttcatacaaa ctttttaatt tagaaaaatc
     9061 atccacaata tcatctcctt aaagttcttt taaatatatt tttacaaaaa gcatctaatt
     9121 gtatgcaaat tgacaatgta aaataaagga aatgaacata aaatgtagaa taatttttat
     9181 atgtatgtaa aaataaaaag aaaaagttga aatttttaga aaataagagt attttttatc
     9241 taaaaagaga gtttaaaaaa taaaaaaatg ttgggaggat aaaaaatgag tttattagaa
     9301 agtatatcaa aaaacatata ttccctagat aactcttcta ttgaaaaaac aaagcagaga
     9361 ctggataggc ttatacatcc aactggaagc ttaggaaaga tagaagatat ttgcatgcaa
     9421 ttagcaggaa tatttggcaa tgaaaatttt gacacaagta aaaaggttat aattgctttt
     9481 gcgggagacc atggtgtata tgaagaaggc gtagcacctg accctcaaaa tataacaaaa
     9541 ttacagtttc caaatttttc gaaaggtcta tgcggagttg gagtaataag taaatttgta
     9601 ggggcagatg ttgtagctgt agatgtagga ataaactgtg atgaaaaact ggatggggta
     9661 ttagactaca agataagaaa agggacatca aatatggcaa aaggtccagc tatgagtaaa
     9721 caagaagcaa ttagatgttt agagataggt attgaaatag cagagcagtg tatagaaaaa
     9781 gattataagg ttattggtat tggggagatg ggtatagcaa atactactcc aagtacagca
     9841 ataatatctg taatttcagg gtgtgacccg ttagaagtta ctggtatagg tgcaggtctt
     9901 aagaaagaaa gactaaagca taaggctgaa gtaattagaa aggctattga aattaataat
     9961 ccaaatccaa ctgatggagt agatatttta tctaaagtag gaggatttga aataggctca
    10021 atggcaggtg ttatattagg ttgtagtgct aataggattc cagtagtaat tgatgggttt
    10081 atttcttatg cagcagcatt aattgcatat aagataaatc caaaaactag agaatatatg
    10141 atagcatctc atttatcagc agaatcagga actaaaagag cattagacat attaaaatta
    10201 gaccctctat taaatatgga tatgagatta ggtgagggaa gtggagctgc tttagcattt
    10261 gatataatag aagcttcaaa ctatacttat aaaaatatgg caacttttga tgaaattgac
    10321 atgggaagat aaatataaaa atttaatatg taggagtgtt ttgatgagta aaattattct
    10381 agtaacagga ggagctaggt cagggaagag taactttgca gaaaaactat gttttgatag
    10441 aaataataat acagcttata tagcaacctc tattccattt gatgatgaaa tgaaagatag
    10501 agtaaaaaaa catcaagaga atagacctag aaactggaat acttatgaaa tatatgaaga
    10561 tatatattcc ataattaagg agatatataa agaacatgaa actgttatac tagattgtgt
    10621 aacattatta gtaaataatc taatgtttga acacaatcta aatatagaag aatctagtca
    10681 agaagaaata aacaaattag aacaatacat aaaagaacaa gttgtcaaac ttattgaaga
    10741 aatagaaaaa acaaatctat actttgtatt tgtaacgaat gagttaggta tgggaattgt
    10801 tcctgggaat aaacttagta gaatttatgc tgatattgta ggtcgtataa atcaatatat
    10861 agcttcgaaa agtaatgaag tttattttgt agttagtggc atagctatga agataaagga
    10921 gtagtatgca attatgaaaa gatttatttt aatactacag tttttgacaa ggatacctat
    10981 aaaattaaat gtaggatttg atgatgaatt ttataagtca atagtatatt ttccacttgt
    11041 aggttttgtc ataggtatat tatcctattt aataggatgg atttctatgc ttttatttga
    11101 accatttata gcttctataa taataactct agctggtgtc ttaattactg gaggacttca
    11161 tatagatgga ttaggagata catttgatgc aatatatagt tatagggata aagaaaagat
    11221 gcttgaaata atgaaagatt caagattagg tacaaattca cttctagcaa ttatgtttgt
    11281 acttttatta aaggttggat tcgtttacga tataataagt aataattcat tgtgggttat
    11341 aatttttatg ccaatgattg caaggttagg agtaatgtta ttgacatata aaactgtgac
    11401 accaagagaa aaaggaatgg gaaatttatt tataggaaaa ttaactacaa gtatgttaat
    11461 aactgctata atatacacat tattgatagt tgctcttatt actaaattta tatttttatt
    11521 acctaatata gtgttgatta aagtattagg ttcgattata gtggtttttg tatttataat
    11581 attattcaaa aaacatattt ataaaaaaat tgacggagta acaggagata tattaggctg
    11641 tggaatagaa ctttcagagc ttgtatattt aatttacata tatttgttaa tttttatgtt
    11701 tttttaaagt aaaaagtggt gataatatga taagattaat attaataaga catgcactga
    11761 caaatgataa taaaaaagga agattatcag gccatataaa tagttgtatt agtgaagagg
    11821 gcaaactaca aataaataaa attaccagat atctgagtaa tgaaaacata gataaggttt
    11881 atacaacacc atctacacgt actaaagata cagtagaaaa aatatcaaaa ttgaaattat
    11941 tagaaataga agaaaaagaa gctttaagag aaattagttt tggagacttt gaaggaagaa
    12001 cttttgaaga aattaaaatc aaatatccaa atgagtttga aaaaatgata aaagaaggta
    12061 acaactatag atatccaaat ggagaaagtc tgatagactc ttataaaaga gtggcgaaag
    12121 aaattgataa tataatttta gaaaatgata gtaatttaga tacaaagaca atacttatat
    12181 gttctcatgc aggaacaata agaaatataa taacacattt gatttctggt agttataagt
    12241 atcactggaa ctttaaaatt gataatgcat ctattacagt tttagaaatt gatggtggat
    12301 ttgctgtaat agataaaatg aattttacag acttcatata atttaatcaa aaccaaacat
    12361 tacaatctgt gtttgtaata tatctgtaaa attagcaatg aaaatagaat attagattaa
    12421 aattaggttc ttaattagat gttaaaaggg aaaaaggtta gatgcctttg cagcccccgc
    12481 tactgtgaaa ccaacgaaac tataaatacc actgtcagtt tgatgggaag gttatagaag
    12541 taggatgaag ttaagccagg atacctgcct aatttaattt aaaacaagag gtcttcgggg
    12601 tgaaagattt ttaattaata tatataaaag tcctatcttt agaagtatta ggactttttt
    12661 ttatttgttt tttagtaaat tttaacaagg agggatggaa agtggggaaa aaaataatgc
    12721 tacaaggaac agcttcaaat gtaggtaaaa gtgtcttagt agcaggtttg tgtagaatat
    12781 ttaagcaaga tggttatact gttgctcctt ttaagtcaca aaatatggct ttaaattctt
    12841 ttataactaa agaaggatta gaaatgggaa gagcacaagt atttcaagca gaggctgcga
    12901 aaatagagcc aatagctgat atgaatccag ttttattaaa accttctgga aataataaaa
    12961 gtcaagttat tgtaagaggt aaagttgtag gagaaatgcc ttcttctaag tatcatgatt
    13021 ataaattgga gttagtagat gtgttgaagg aaacatttaa tagcttaaat tcaaggtatg
    13081 atattgtagt tatggaaggg gcaggaagta atgcagagat aaacttaaaa gatagagata
    13141 tctctaatat gggtatggca gaaatagctg atgcacctgt aataatagtc ggagatattg
    13201 atagaggagg cgtatttgcc tcattagcag gaacaatgct tttattaaat gaagatgaga
    13261 aaagaagagt aaaaggtgta ataatcaata aattcagagg aaaaaaagag cttttacaag
    13321 atggtgtaaa gatgctagaa gacatcataa aagtacctgt tataggagta gtaccttata
    13381 cagatataaa aatagaagat gaagacagtg taactacgag atttaagaaa aaaatgaata
    13441 aaagtgatat acacatagaa ataattagaa ccccacatat gtcaaatttt acagatttta
    13501 atatatttga gacacaagaa gatgtaagta ttagatatgt agactatgga gaatctttag
    13561 gaaatccaga tatattaata atacctggaa ctaaaagtac tatagacgat ttaacatata
    13621 taagaaaaag tggtcttgaa tcgcaaatca aaaatttaca tagtcaaggt aaattgatat
    13681 ttgggatatg tggtggatac caaatgttag gtaaaaaatt aaaagaccct tatcatgttg
    13741 aaagtgaaat tgaagaagtt gatggaatag gtcttttaga tacagaaaca acgtttgaga
    13801 aagaaaagac tacaacacaa gtagaagcta ctattgttga atgtatcagt gaatacatga
    13861 ataaccttaa gggaaaaaaa gtaaaaggtt atgaaattca tatgggaatt acaaagcgag
    13921 gtaatgatgt aagaagttta gatttaataa ctaaaaaact tgataaagaa gtgaattata
    13981 ctgagggaag tgtaaataaa gaggggaatg taattgggac ttatcttcat ggaatatttg
    14041 atgaaataag ctttacaaga gcaattttaa acaatataag aaagaaaaaa ggtttaggag
    14101 aattagaaag caaagtaaac tcttttaatg aatttaagga taaagagtat gataagttgg
    14161 ctgatttttt aagagaacat ttagatatag agaaaatata tgagattatg aaataatgat
    14221 gacttttatt tataatttag gtaggaggtg tattatgaaa aaaattttaa ttgcgggtac
    14281 taatagtgga gtaggtaaaa caacaatatc attaggaata atgcaagctt tggttaagag
    14341 aaatatgaaa gtacagccat ataaagtagg gccagactat atagaccctt catatcacac
    14401 ttttataact ggaagacatt ctagaaattt agattcttat atgttagatg atgaaaaaat
    14461 aaagtatgta gtaaataagt cctcaaaaga tgcagatatt tcagtaatag aaggtgttat
    14521 gggcttatat gatggttttg gaatagattt agataattgt acaagttcac atacttcaaa
    14581 ggtattaaag tcacctgtta tattagttat aaatggtaaa tctatggcag catctagtgc
    14641 tgctatggta cttggatata aggaactaga taaagatgta aatataaagg gtgttatagt
    14701 aaataatgta aagactaata gccattatca aataattaag agttctatag aaaaatattg
    14761 taatgttgaa gtattggggt attttcctcc aaataacaaa ttctctttgg aatctcgaca
    14821 tctaggatta gttcctagtg tagaaatgaa atcactaagg gaaaaatttt ataccttagc
    14881 agatgaaatt gaacactata tagatattga cagaataatt gaaatatcag aaagtgatga
    14941 atttgagagt gattttgatt ttcaaacttt gatagaaaat aataaaataa aagaacatgt
    15001 taataataaa tcaatagcta tagcatatga taaagcattc aatttttatt atagagaaaa
    15061 tatagagctt ttagaggaac ttggtttaga aataaactat tttagccctc ttgaagatgc
    15121 atcagtgcca aatgcagatt atatttatat tggaggaggt tttccagaaa tatttggaaa
    15181 agaattagaa gcaaatgagt caatgagaac ttccatatta gaagcacata acaataatat
    15241 acctatttat gcagaatgtg gaggactgat gtacctaggt gagaaactat tgaatcaaga
    15301 agaacaagaa tttaatatgg ttggaatatt tagtggaatt agtaagatga catcttcttt
    15361 aaagagattt gggtactgta ttggtgtagc caaggaaaat acttttttat ctaaaaaagg
    15421 ggaatccatt aaagggcatg agtttcatca ttcaatattt gaaagtgatg aaaaatgtgt
    15481 atattcaatg agaaaagaaa gagatggaaa tatagtagaa gagtgggatg gtggttatag
    15541 taaaggaaac actttggcta catatcttca tactcatttt tataataact taaattgtat
    15601 agaaaatttt ttgaaaaaag gaaatgaata aatgagtatt ttatctattt atataggata
    15661 tgtgacagac ttaattgtag gagacccata ttcttttcct catccagtga gatttattgg
    15721 taaacttata aattttactc aaggtataat aagaaaggtc tctaaaaatg ataggcagtt
    15781 aaaatttggg ggatttatat tatggtttat aaccgttgga ataacatatt taacaactta
    15841 tgctatagtg aagttattta gttttaatat attattgagt attgtagtga atagttttat
    15901 aatttataca acactagcca caaaatgttt aaaagacgaa gcattaaaaa tttataatgt
    15961 attaaaaact gaagatatag aaaaatctag gattcagcta tcttatatag taggtaggga
    16021 tactactaat ttaagtgaac ctgaaataat aagagcaact gtagaaactg ttgcagaaaa
    16081 tactgtagat ggtataattg cgcctatgtt ttatgcattt attggaggag caccacttgc
    16141 tatggcctat aaagctataa atacattgga ctcaacagtt ggatataaaa ataaaaagta
    16201 taaagatatt ggttttgcat ctgctaagat tgatgatata gccaattata taccagctag
    16261 aatatctgtc attcttatga caattggaag tttcttttta aattataact ataggaactg
    16321 ttttaaaata tctattagag atagaaagaa tcataaaagt ccaaattgtg ctttttcaga
    16381 aggtgcagta tctggtgctc taggaataca actaggaggt accaacgttt attttgggga
    16441 aaaagtatat aagccaacaa taggggataa gcttagggag atagataaag aagatataat
    16501 taaaactaat aaaattatgt atgcatcttc atttgtatct atgttggtat ttacactaat
    16561 atatgtgtta tgtaggatga ttttaattaa cttaattata taaattattg tttgttaact
    16621 taagggggac atagatgaag gatttagggc atggtgccaa tgtagatgaa atggcaagat
    16681 tatatgggaa aaacccaaaa gaaataattg attttagttc aaacataaat ccaaatgtat
    16741 taccaaattt agagagatac attttaaaag gattagaaga gtgtaggaat tatccagata
    16801 taaattatac aaacttgaga gaaaatatat caaaatatat agatataaat ccagacttta
    16861 taatacctgg caatggtgca acagaagtga tttatttgct tatgaagagt attaaaaaaa
    16921 aattggctat aataaatccg actttttcag agtatagaag aagtgctgag ttaaataatt
    16981 tagatattat agatttagag ttagatttag aaaataactt taaacttaat atagatataa
    17041 taaaagaaaa tataaaaaga tttgatagtt tatttatatg taatcctaat aatccgagtg
    17101 gtaatgtcca agatttaaag gaacttgttc atttactaga taaacataat aaggtactaa
    17161 taattgatga aactttcatg gaatttgttg aagatgaaag taaatatagt ttggttaaat
    17221 atatagaatc aaataaaaat atatttataa ttaaagctgt gactaagttt tttggtatgc
    17281 ctggtttaag gctaggatat ggtcttacaa gcaacactga aattatgaac aaaatttatg
    17341 aacacaaaga gccatggact atcaattctt ttgcagatat actatccaat tttatttttg
    17401 aagataaaga gtatattaaa aatagtaaag aatattacat agaagaaaga aaatatatgt
    17461 tacaagagtt gagaaacata agaaatataa aggtctatga tacagatgct aattttatct
    17521 tgataagaat atataaaaaa acaactaaag aattgaaaaa agatttgttt aagcaaggaa
    17581 acatattagt tagagatgcg tctaatttta taggattaga tgatagtttt ataagagtgg
    17641 ctataaaatc tcatgaagat aacaaaattc ttatagaaaa tatgaaaaat ttattgggtg
    17701 attaaaatga aatcttatgg aatatgccca gcatcttgtg gagaatttgt acaaggtata
    17761 attgatgatg aagaatattt atgttcatat gcaatagata tgtattctaa agtgtatatt
    17821 gaagaaaaat tagtagatat aaatttaggg agatataagt caaggttagc aattgaaaag
    17881 gtatttgaaa aatttaattt acctaaaaaa tatactaaaa atatttcttt aaatataaat
    17941 agtaaaatac ctgttggaaa aggaatggca agttcaactg ctgatatagg agctactata
    18001 aaggcaacat tatcattgat agataaagat ttaagtagcg aggagatttc taaattagca
    18061 gcagaaatag aaccaacaga ttctatattt atagataaaa atagtatatt taatcctcta
    18121 aatggaactg ttataaaata cctaggaaat ctgaccaatg cgaaagttgt aatacttgaa
    18181 ccaaacaaag tactagatac tatgaagatt agattgaggc aagattataa taaattaaaa
    18241 gtagagaata aagaagtaat aaaaaaatca tttgctcttt tagaagaagg attgaaaaaa
    18301 aataatttat ctctagttgg agaggcttgc actttgagta gcttagcaaa tgagaatata
    18361 gaaaaaaaag agtgtttaaa tgaaataata aaaatatcaa aaaaatatgg agcatatgga
    18421 gtaaatattg ctcacagtgg aactgtagta ggtattttaa ttgataagtc tatgaatgat
    18481 aaaaagatga tagatgcctt atgtgaatct aacataaatt ctgtctataa taaaatttat
    18541 acacaaaaca taattaatgg aggaattaag ggggaaatag aatggaatac ataaagaatc
    18601 ctatgaaaat agaagaaaaa agttttgaat tgatacaaga aataatagac gaaattagac
    18661 cagattataa atttaagaat aatatcgaag aaaaaataat aaaaagagct atacatacta
    18721 ctgctgattt tgattactta gatatattaa aaatatcaga agaagcagtt gatagtataa
    18781 tagatgcatt aaaaaataat gcaagtattt atactgatac aaatatggct cttagtggta
    18841 taaataaaag aaagttagaa tcattaggat gtaagtataa atgtttagtg gcagatgatg
    18901 aaactgtaaa attagctaaa gaaaaaggaa ttactcgttc tatggcagca gtagaaattg
    18961 cagctaaaga agaaggaaga aaaatattcg ttttaggtaa tgctccaaca gctttatata
    19021 aagttatgga gatgaaatca gaaggtagat tagatttaga tgcagttata ggagttcctg
    19081 taggttttgt aggagcacaa gaatcaaaag atgaagttga aaaaacagat ataccatata
    19141 taatttctaa aggtagaaaa ggtggcagta atttagcagc agcaatagta aatgctatat
    19201 tatatactat gtaggtacct atatggaaga atatgtatat atagatggaa aaaaatatag
    19261 aagaggatat actacaggtt catgtgcaac aggtgcatct aaggctgctg tatatatgtt
    19321 gattacaaaa aatagaatca atactataaa tatagatact cctaagggga taccattact
    19381 actaaaagtt gataatataa atatttttga tacatttgtg gaatgttcca taaaaaaaga
    19441 tggtggagat gatatagatg caactcatac catggatatt tatgcaagag ctgaaattgt
    19501 agctaaaaat gataagaaca aagggtatct tactctaaag gatatagata gtttaagcac
    19561 caatagtgag tgtaaaagtg agctctataa gtttattagg gtttatggcg gaactggtat
    19621 tggtgtggtt acgaaaaaag ggcttagtgt ggatgttggt aagcctgcta taaatccgac
    19681 tccacttaaa atgataaatc atgaaataag aaagcttata ggagataatt ttgaatctat
    19741 acttggaaat gataaagttt taaaaataac aatctttgct ccacaaggag aaactgtagc
    19801 aaaaaaaaca tttaatccaa gattaggtat agttggagga atatctataa taggtacaac
    19861 tgggattgtt gaacctatga gtgatgaggg ttggaagaag tctctatcta tagaacttca
    19921 gatgaaaaaa gaacaaggct tagataaaat aatattggtt ccaggaaatc atggagaaca
    19981 gtttataaga gaaaaattaa acttagatat taagtatgta gttagggtaa gtaattttat
    20041 aggctatatg attaaagaag ctcaaagaat aggatataag aaaatcttaa tggcaggtca
    20101 tataggtaaa tttataaaag tatctgctgg tatttttaat actcatagta aggtagcaga
    20161 tgctagaagt gagatattag tggcaaactt agctttaatg ggagctagat acgaattttt
    20221 aaataaaata aatcaatgcg taactactga agaagctgta gagctaataa ataatagtga
    20281 atatagagaa gtatataata tattaagtaa taaatgtagg gagagagtaa agcaatactt
    20341 gaatgaagat tcagatgata ttgatgtgga agtaatcata ttttcaatgg ataagtcttt
    20401 attaggtaaa tcggataata cagatgattt agtggaggtc tttatatgat aaacataata
    20461 ggtctaggac ctggtaactt agactatata acaaaaaaag gtgaaaattt aatttccact
    20521 tctgatgtat taattggtgg taaaagaaat ttagagtcta taaaaaattt tgaaggagaa
    20581 aaaatagttc tggattctaa tttaagagaa attatagagt acataaataa caataaagaa
    20641 aagcaaatat caataatagc atctggggac ccactaatat atgggatagg tagatatcta
    20701 tctaaaaata ttgataataa gatgttaaac atggtttctg ggattagttc attacagtac
    20761 atattttcaa aaatatatgt agatatgaat gatgtatata ttacaagtag tcatggtaaa
    20821 gtaccagatt ttgattacat attatcacat aaaaaagtat gtatggttac agattcaaag
    20881 ataggaccaa aacaaataag tagagaaata atagatagaa atctaaataa aattattgtt
    20941 gtaggagaaa atctgtcata tgacaatgaa aaaataacaa tagcaaaacc agaagagata
    21001 ataagaatgg acaattttga tatgaatgtt gtggtgattt tagatgagga atagtgaatt
    21061 tataacaggt aaagttccaa ttactaagga agaggttaga gcaatatcaa taagtaagtt
    21121 aaatttagta aatgctaaaa acctcataga cataggggct ggtacaggaa gtgtaagtgt
    21181 tgaagctgca tataattatc ctaatttaaa ggttatatct atagagaaaa atgatgatgc
    21241 tatagagctt atagagaaaa ataaaaaaaa attcaactta aaaaatatag aagtgataaa
    21301 aggatatgct ccaattaatc tagggttaaa tgttaaagtt gagtctattt ttcttggggg
    21361 aacaggaaat aatctggaag aaataataga atggtctaaa aaaaatcttg ttacaggggg
    21421 aagacttgta gctaatttta taataataga tacatttaat caaactttaa aactgcttaa
    21481 aaagcatgga tttaaagaaa tagatgtatg tgttttgaat gtttctaaac tggagaaact
    21541 aggaaaaggt gaatatttta aaccactaaa tcctatatat ataatatctt gtgaaaaagg
    21601 ggaaaatgat gatgaataaa gtacattttg taggagcagg acctggagat aaagagctga
    21661 taacattaaa aggatacaaa ttattaagta atgcagatgt agtgatatat gcaggttcac
    21721 ttgttaatcc tgaacttttg gaatactgta aagaagattg ccaaatacat aatagtgcac
    21781 atatggattt acaagagata atagatgtta tgagagaagg tatagaaaat aataaatctg
    21841 ttgtaagact gcaaacaggt gatttttcta tatatggttc aattagagaa caagtggaag
    21901 atttaaataa attgaatata gattatgact gtactccagg agtaagttct tttttagggg
    21961 cagcatcatc tttaggggtt gaatatacag ttccagaaat ttctcaaagt gtaattataa
    22021 caagaatgga aggcagaact ccggttccag agaaagaatc aatacaatca tatgctaagc
    22081 atcagacatc tatggtaata tttttatctg ttcaagaaat tgagaaagtt gtgtctaaat
    22141 tactggaagg tggatatcca aaagatactc ctatagcagt tatatacaaa gcaacttggg
    22201 cagatgaaaa aatagttaaa ggaactttaa gtgatatagc tgtaaaagtt aaagaaaaca
    22261 acataaataa gactgcttta ataatggttg ggagattttt aggagaagag tataataatt
    22321 caaagttata tgataaggat ttcaaacatg aatatagagg gtaatattgc tataatctgt
    22381 attacagaaa atggaaaaaa attagctcta aagataaatt ctctagtaaa taactgtgtt
    22441 atatatcaag taaaaaataa aaaaagtaat ttagttgttg aagaaacatg tgtgcataca
    22501 gttcaaaaga agttaagtga ttttgtagga gaaatattta gcaaatttga ttacatagta
    22561 tttataatgg ctacaggtat agttgtacga agtatagcac catacataat aagtaagttt
    22621 agtgaccctg caattttggt aatggatgaa aaaggcaaaa acgtaataag tttattgagt
    22681 ggtcatattg gaggggcaaa cagcttgaca cagtacataa gtagtattat tgatgctaat
    22741 ccagtaataa caacagctac tgatataaat caaaaagctt ccttagatat gatagcaaaa
    22801 aaactaaatg cacatattga taattttaga gaaagtgtaa aagaagtaaa ttctatgcta
    22861 gtgaataata aatgtgttgg attatatgtt gatgaaggat atcaagttga tactagggga
    22921 tttaaactta tagaaagtaa ggatgagtta agagatagtt tttcaaacac atatatagaa
    22981 aaattagttg tagtaagcaa taaaataaat tgtattgaag atattgagaa tttaaaccta
    23041 gataataaat ttggacattg ctatagtacc aaaaaaataa taaaggtaat tccaagagat
    23101 atagtgatag gaattggttg tagaagaaac acagatagtg aactgatgta taattcactt
    23161 gaagagtttc ttaataaaca aaatatagat attagagcaa taaacacagt tggaagtata
    23221 gaactgaaaa aagacgaaaa agcaataatt gaattatcaa aaatattgaa ggtgccattt
    23281 aaaacatttt cagtagatga aatatctaag gttgataatc tttttgataa atctgatttt
    23341 gttaaaaaaa atgtgggcgt gtattcagta gcagaacctg tagcatatct tttgagcaaa
    23401 ggtaatttgt tagttgaaaa acataaatat aaaggaataa ctttttcagt ggggagaata
    23461 aaaatatgat atacgttgta ggaattggac caggaagtaa agatacaatg actttagaag
    23521 caataaaagc aatagaagat tctgaggcta ttgttggata taagacttat ataaaactaa
    23581 tagaagaatt tatacaagat aaagaaataa tccaaaatgg tatgaggcaa gaagttgata
    23641 gatgtaaaca agcagtagag gaagcaaaaa aaggaaaaaa agtagcagtt gtaagcagtg
    23701 gtgatgctgg aatatatggg atggcaggtc ttattttaga attaatatct aaagaagatg
    23761 aagatataaa agtaaaggta attccaggtg ttactgcaag tattggtgca gcagctatac
    23821 ttggagctcc tattatgcat gatttctgtc atataagttt gagtgattta atgactccgt
    23881 gggaagtaat tgaaaaaaga ttgagattgg ccgctgaagc cgattttgta atttgtttgt
    23941 ataatccaag aagtaaggga agaagtgaac atctaagcaa agcatttaaa ataatgggtg
    24001 agtttaaagg tggagataca cctgttggag tggtaaaaga tgtaggtaga gaaaaagaag
    24061 aaaagtttgt atgtactttt gaaaatatgg actttgaaaa agtcgatatg actacaatgg
    24121 ttataatagg aaataagtct acctatatta atggtgaatt gatgataact ccaagaggtt
    24181 atacagtatg attttaattt taggaggaac ttcagatagt ttatctatat gtgataagat
    24241 aaatgaactg aaaaatcaac catatatcct ttcagttact acagattatg gaagggaact
    24301 tgcacaacaa catgcagaga atgtaatttt aggtaagctt ggaaaagaag atatgctaaa
    24361 atttataaaa gataataata tagtaaaaat aatagatgcc actcatcctt atgcagttga
    24421 agtatccaaa actgctatta cttgtgcaaa attattgaat atagattata taaggtttga
    24481 gagaaaatct ttaattgaag aaatagatta tgataataaa tttatagtag atacaattga
    24541 agaagcctgt gaaatagcaa ataaaatagg gacaaatata tttataggaa caggaagtaa
    24601 aaatttgaac aaatttatag aggaaataag tgataaaaat ttagttgtta gagttcttcc
    24661 aacgagtgaa gtcatattaa gttgtgaaaa attaggtcta agtgcagata atataatagc
    24721 tatgaaaggc ccatttagcc agtatataaa tgaagaaaca tataaacatt acaatataga
    24781 tttagtcata acaaaagaaa gtggaatagc tggtgggttc ttagagaagg taaatgcatg
    24841 taaaactttc aatataccag taataattat aaaaagagaa tcgattaact acgaaaatgt
    24901 atttaatgac ataaataaaa ttaaagacat acttgtgtaa tttttaggag gagaatatat
    24961 gaagaaagcc ttattagtag tgagttttgg tacaagctat catgaaacga gagaaaagac
    25021 aatagatata tgtgaagata aaataaaaaa ttcgttaaaa agccatgatt tttttagagc
    25081 ttatacatca aatatgataa taaataaaat aaaaaaaagg gatggaatag aaatagataa
    25141 tccaatacag gctttagata aaatatatga acaaggatat gatgaagtaa taatccaaac
    25201 acttcatatt atatgtggcg aagaatttaa caaattaaaa gaacaagtag atggatattc
    25261 ttctaaattc aaaaagattg tattaggaag acctttgctt acacatattg aagattatca
    25321 ggaagctgta gaggcaatta agcatcaaat tcctcatatg gaaagtaatg aagcagtagt
    25381 atttatggga catggaacac ttcatgaatc acattcagcg tatccagcat tggaatacat
    25441 gcttagggat aatggaataa atgcttatgt tggtacagtt gaagggtatc cagaaataga
    25501 acatgtaata agaagattaa aagaaggaaa tataaaaact gtaaatttga tgccatttat
    25561 gttagttgca ggagaccatg ctataaatga tatggcagga gatgaagaag attcttggaa
    25621 aacaatatta gaagaaaatg gatttgacgt aagagttcat ttaaaaggac taggagaaaa
    25681 ttcttatata caagataaat ttgttcgtca tgctgtaaaa tgtgcagaga atgcgaagtt
    25741 ctatggtata ggagcagggc caggagatgg ttctttactt acgataaaag cggttaatac
    25801 tttaaagaaa ctggatattc tatacactcc agaatcaaaa aagggtggag atagtctagc
    25861 tttatcaata gttagtgaat atttacctga atcagtggaa ataaagtcta ggcatttccc
    25921 aatgagtttt gatgggaatg aaaaaagttt agcttgggat aatgtagcac aagaaattat
    25981 atcagatgtt aatgatggta aaaatgtagg atttgttaca ttaggagacc ctatgattta
    26041 tagtacatat gtatatataa tgaaaagaat attaggagag gtagaagtcg aaactatacc
    26101 aggaatttca tcattttcca atatagcatc taatcagaat tttccattag tcatggacaa
    26161 agaagcttta gtggtaattc cttgtactat ggaagatgat aagatagagt atgctttgca
    26221 aaattataat tcaatagtat tgatgaaagt atataaaaat tttaaagaaa taataaaaaa
    26281 attagaaaag tatgatttaa tagaacatgc tattttagta agtaattctt cccaagaaag
    26341 cgaaaatgtg tttacagatt taaaagaagc gcaccttgaa gataagatat cttatttttc
    26401 tactatacta gtaaataaag ataataaaat aaaataggtg aaagcaatat ttaaaggaga
    26461 cttaatatga atatagtggt tggaactagg ggtagtaatc tagcgcttat acaaacagaa
    26521 tgggtaataa atgagttaaa aaagaagtat ccagaaatta gttttgagat taaaatcata
    26581 aagacaaaag gagatttaat tcaaaatgtt tcactggata aaataggtga taaaggattg
    26641 tttgtcaaag aaatagagca acaattatta gatggaaaaa tagacatagc agtacatagt
    26701 atgaaagata tgccttctta tttagcagat ggactaaaat ttgctcatac gcctaagaga
    26761 gaagacccaa gagatgttct aatacttaga gaaggatata aaaatttaga tgatttgcct
    26821 catggagcag taataggaac aggaagtaaa agaaggaagt ttcaattatt gaaacaaaga
    26881 cctgatttaa atatagtaca agttagaggt aatgtggaaa ctaggatacg aaaaataaaa
    26941 gatgaaaaca tgcatggtat tgtgttggca gcatcaggaa taattagagc taatttacaa
    27001 gataaaataa gttcttattt acctgttgat gttgttattc cagcacctgc ccaaggagct
    27061 ttagctatag aaattagaag caatgatagt acgatagagg gaatagtaaa ttcactgaag
    27121 gatgagaata cagaaatcca aatattggct gaaagaggtt ttttagatgg tgtaaatgga
    27181 agttgtcata ttccaatggc agcttattgt gaaataatcc aagataaaat acatcttact
    27241 ggcttatatg gagattcaga aggaaaaaaa gtagtaatca agtctataga tggagatatt
    27301 tcttctccta gagagttagg tttaaagtta gctaaacttg tattgaagga gtatgagaat
    27361 tatgaagggt aaggtttact tagtaggggc aggaccaggt gattataaac tcatgacttt
    27421 aaaaggttta gagtgtataa gaaaatcgga tgtaatagta tatgatagat tagcaaatag
    27481 tagttattta agagaagcta agcctgattg tgaacttata tatgtaggca aggcatcaag
    27541 taatcatatt ttgccacaag aagatataaa tagaattata gcagaaaaag ctaaagaagg
    27601 aaaaattgta actcgtctta aaggaggaga cccttatgta tttggcagag ggggagaaga
    27661 agcagagact ttagtagatg aaggtataga atttgaagta gttccaggta taacttctgc
    27721 tataggaggt ttatgttatg cgggtattcc tataacacat agagaccatg cttcgtcatt
    27781 tcatgtaata acaggtcatt tgaagggtga tgatagtgga gaattaaact ggaatgctct
    27841 tgcaaataac aaaggtactc ttgtgttttt gatgggaatt tcaaacctag agaaaataag
    27901 tgaaaactta atgaaagaag gaaaagataa ggatacccct gttgctctta taagttgggc
    27961 aactagatat aatcaaagag ttgttacatc tacacttgaa aatgtttatg aaactgctat
    28021 taaagaagaa gtaaaaccgc caactcttat agtagttggg agtgttgtag gacttagaga
    28081 aaagttgaac ttctttgaaa gtaagccttt atttggaaaa agtattgctg taactaggtc
    28141 tagaaatcaa aatagtgtat tggtagaaaa aataatggat ttaggtggaa atcctataga
    28201 aatacctact attaaggtag aaaaaataca aaataatatt aacttagaaa atgaaataaa
    28261 aaatataaat aagtataatt atctaatact aactagtaaa aatgccgtag aaatcttctt
    28321 tgaaaagata tatgaaatga attttgattt aaggatatta tctaatttaa aggtatgtgc
    28381 tataggttct gctacatcaa atgagttgaa aaaaagagga attatagcgg atatagtacc
    28441 aaaaaaattt gtagcagagt cactgtatga agagttagtg cctattttaa atgataacga
    28501 taatatactt atacctagag cagaaaatgc tagagattat ctggttgaca aacttaaggc
    28561 tatttgtgaa gtaacagaag tacatatata tagaacagta atagatgagg agaaaaaagg
    28621 tgagatatta gatattttaa attctaatga catagattat ataacattta ctagttcctc
    28681 aacagtaaat aatcttgttg aaattatagg aaaagaaaat attaaaatat tagaaaaaat
    28741 aaaaacacta tctataggtc ctataactac agagacaata aatagtttgg gaataaaatt
    28801 gtacaaagaa tctgctacat ctactataga ttcgttagta gatttaatta taaaaaatta
    28861 attttggagg cttgaatatg ttaagaagac caagaagact aagagcaagt aaagcaatca
    28921 gaaatttggt aagagaaact aaggtgaatg tagaagactt aatatatcca ctgtttgtag
    28981 tagaggggga aaatataaaa gaagaaatta aatctttacc agatgtctat catttatcaa
    29041 tagacatgct agaagacgaa ataaaagaaa taagtgagtt gggaattgaa catataatgt
    29101 tatttggtgt tccagatgaa aaagatgagt gtgcttcaca agcttttaat gataatggaa
    29161 tagttcaaaa ggcaattaga aaggtaaaag aaataaattc agatataaat gtaataacag
    29221 atgtctgtat gtgccaatat acaagtcatg gacattgtgg gattttaact gatactggtt
    29281 atgttgataa tgataaatca ttggagtatt tggctaaaat atctgtaagc catgctaaag
    29341 caggagctga tatggttgct ccatctgata tgatggatgg aagaatacaa gcaataagag
    29401 aagctttaga tgaaaatgga ttagaaaatg ttggaatcat ggcttatagt gttaagtatg
    29461 cttcaaatta ttatggacct tttagagagg cagctcattc agcaccatct tttggagata
    29521 gaaaaacata tcaaatggac ccagcaaata gcaatgaagc tataatagag actgaacttg
    29581 atatattgga aggtgcagat atacttatgg ttaaacctgc attatcatat ttagatatta
    29641 taagaagagt taaagataat tttgacttac ctctagcagc ttataatgta agtggagaat
    29701 actctatgct taagtttgct gtgaaggaag gtttattaaa tgaagatgta attcttgaat
    29761 caattacatc tataaaaaga gcaggagcag atataataat aacttatttt gctaaagaca
    29821 ttgctaaact gttgaaaaaa taaattctag tagtataaag aatatcaaaa agtataggaa
    29881 ttgtaattaa ctattataca gtagttttgt ttagacattt attgaagaat aatagaaact
    29941 aatttgcaat ttctatactt tatttatgta actaaataaa atttggaggt ggaagatgct
    30001 atatcctatt aatttaaaat tagatgagtt agatgtagtt ataattggtg gtggagaagt
    30061 tgcatatagg aaatgtaaaa attttttaga gtttaacaaa aatgttacta tagtttctaa
    30121 acaaatttta aataaatttt atgatttaaa aggaaatata aaaataataa aagatgatta
    30181 taaagaaata tatataaaag atgcttctat aataattgct gccacaaata atagagagtt
    30241 aaatatggaa ataggattat attgtagagc aaaaaataag ctagtgaatg tagttgataa
    30301 tatagaaata tcgaacttta cggttccatc atatataaaa agaggagatt tacttataag
    30361 tgtgtctact gggggcaaga gtccttcatt atcatcaaag ataaaaaaag aaattgaaga
    30421 gagatatact gaagattatg aggagtattt aaatgtatta ggagatataa gaaaaaaggt
    30481 tataaaaaaa tacgaagata aaagtaaaag aaaagacgtg cttaatgtgt tggtaacact
    30541 tgacctagaa gaacttaaga agtttgatat ataaaaaata aaataaaaaa tgttaaaaag
    30601 gtgttgacgg attattttta agatgatata gtattacttg tccttaagaa aagggcaaac
    30661 ataa
//
LOCUS       DACXMZ010000019        30624 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.019, whole genome shotgun sequence.
ACCESSION   DACXMZ010000019 DACXMZ010000000
VERSION     DACXMZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30624
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.019"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(105..890)
                     /gene="aroF"
                     /locus_tag="JD016_03455"
     CDS             complement(105..890)
                     /gene="aroF"
                     /locus_tag="JD016_03455"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAU4998688.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            complement(871..1377)
                     /gene="folK"
                     /locus_tag="JD016_03460"
     CDS             complement(871..1377)
                     /gene="folK"
                     /locus_tag="JD016_03460"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAU4998689.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEKLIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            complement(1370..1735)
                     /gene="folB"
                     /locus_tag="JD016_03465"
     CDS             complement(1370..1735)
                     /gene="folB"
                     /locus_tag="JD016_03465"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HAU4998690.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            complement(1746..2549)
                     /gene="folP"
                     /locus_tag="JD016_03470"
     CDS             complement(1746..2549)
                     /gene="folP"
                     /locus_tag="JD016_03470"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAU4998691.1"
                     /translation="MSLIVFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDM
                     VDQGADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEE
                     ALKLGVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVS
                     IEMAMSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGK
                     VLPVEPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            complement(2601..3173)
                     /gene="folE"
                     /locus_tag="JD016_03475"
     CDS             complement(2601..3173)
                     /gene="folE"
                     /locus_tag="JD016_03475"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAU4998692.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            complement(3199..3900)
                     /locus_tag="JD016_03480"
     CDS             complement(3199..3900)
                     /locus_tag="JD016_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998693.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVVIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            complement(3934..4677)
                     /locus_tag="JD016_03485"
     CDS             complement(3934..4677)
                     /locus_tag="JD016_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAU4998694.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYTKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            complement(4667..6025)
                     /gene="pabB"
                     /locus_tag="JD016_03490"
     CDS             complement(4667..6025)
                     /gene="pabB"
                     /locus_tag="JD016_03490"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HAU4998695.1"
                     /translation="MCNMIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSF
                     ISSQPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENL
                     PRTAVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEE
                     KKGIDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETE
                     LTSFELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNKRIETRPIKGTRPRGK
                     DKEEDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNH
                     LVSTVVGELKDDKDVTDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYI
                     GFNGDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYY
                     EE"
     gene            complement(6018..6602)
                     /locus_tag="JD016_03495"
     CDS             complement(6018..6602)
                     /locus_tag="JD016_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HAU4998696.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            complement(6626..7345)
                     /locus_tag="JD016_03500"
     CDS             complement(6626..7345)
                     /locus_tag="JD016_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAU4998697.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            complement(7373..8437)
                     /locus_tag="JD016_03505"
     CDS             complement(7373..8437)
                     /locus_tag="JD016_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAU4998698.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            complement(8581..9390)
                     /locus_tag="JD016_03510"
     CDS             complement(8581..9390)
                     /locus_tag="JD016_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998699.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            complement(9632..10498)
                     /locus_tag="JD016_03515"
     CDS             complement(9632..10498)
                     /locus_tag="JD016_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAU4998700.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            complement(11018..11707)
                     /locus_tag="JD016_03520"
     CDS             complement(11018..11707)
                     /locus_tag="JD016_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAU4998701.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            complement(11700..12071)
                     /locus_tag="JD016_03525"
     CDS             complement(11700..12071)
                     /locus_tag="JD016_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAU4998702.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            complement(12253..13599)
                     /locus_tag="JD016_03530"
     CDS             complement(12253..13599)
                     /locus_tag="JD016_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAU4998703.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKNEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSIVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            complement(13781..14374)
                     /locus_tag="JD016_03535"
     CDS             complement(13781..14374)
                     /locus_tag="JD016_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAU4998704.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(14439..15908)
                     /locus_tag="JD016_03540"
     CDS             complement(14439..15908)
                     /locus_tag="JD016_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAU4998705.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SGFFGNLSKTISANFGRKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(16023..17414)
                     /locus_tag="JD016_03545"
     CDS             complement(16023..17414)
                     /locus_tag="JD016_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAU4998706.1"
                     /translation="MYFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLVQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKACKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISESGLNEY"
     gene            complement(17580..17870)
                     /locus_tag="JD016_03550"
     CDS             complement(17580..17870)
                     /locus_tag="JD016_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998707.1"
                     /translation="MSGLKKILRILVISMMICFAMTFALVLINSVMGVHIGLDYYFLM
                     GSVLTVCLTIGIVLCNARKIFDSKAIHKTRTKTVSTNKKQKSHSRDIKRKIS"
     gene            18085..20223
                     /locus_tag="JD016_03555"
     CDS             18085..20223
                     /locus_tag="JD016_03555"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAU4998708.1"
                     /translation="MIYMPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGD
                     FTPVCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIE
                     DRDMRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDA
                     LQTSDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYN
                     DEEVSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEK
                     KHTKNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYDKEDSSYEDFYKHNYK
                     NYDYTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPT
                     NVDAQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGF
                     SDAALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLT
                     AIRDVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKH
                     QANLFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDY
                     INKNGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYD
                     QANILARTMSIGINDPEVLKEELEGIYGQF"
     gene            complement(20465..21064)
                     /locus_tag="JD016_03560"
     CDS             complement(20465..21064)
                     /locus_tag="JD016_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HAU4998709.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(21082..24201)
                     /locus_tag="JD016_03565"
     CDS             complement(21082..24201)
                     /locus_tag="JD016_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAU4998710.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPVEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVLDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     NFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            24422..25246
                     /gene="pdaA"
                     /locus_tag="JD016_03570"
     CDS             24422..25246
                     /gene="pdaA"
                     /locus_tag="JD016_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HAU4998711.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(25343..26128)
                     /locus_tag="JD016_03575"
     CDS             complement(25343..26128)
                     /locus_tag="JD016_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU4998712.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(26228..26362)
                     /locus_tag="JD016_03580"
     CDS             complement(26228..26362)
                     /locus_tag="JD016_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998713.1"
                     /translation="MNISRKAMKIIELAQKIANKRGISVEEAWSEAVTEYKNKYEHIA
                     "
     gene            26593..26958
                     /locus_tag="JD016_03585"
     CDS             26593..26958
                     /locus_tag="JD016_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4998714.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            27036..27589
                     /locus_tag="JD016_03590"
                     /pseudo
     CDS             27036..27589
                     /locus_tag="JD016_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
     gene            27809..28483
                     /locus_tag="JD016_03595"
     CDS             27809..28483
                     /locus_tag="JD016_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAU4998715.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      28838..28934
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            28988..29164
                     /locus_tag="JD016_03600"
     CDS             28988..29164
                     /locus_tag="JD016_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998716.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(29351..29590)
                     /locus_tag="JD016_03605"
     CDS             complement(29351..29590)
                     /locus_tag="JD016_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998717.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(29735..30544)
                     /locus_tag="JD016_03610"
     CDS             complement(29735..30544)
                     /locus_tag="JD016_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAU4998718.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT         9980 a         5171 c         3109 g        12364 t
ORIGIN      
        1 tcaatattaa atctttattt atattaaata atctaactat caaattataa cttataaatt
       61 taccttagca acgattacga ataaaaaaga ggctcttgcc tcttttaata attattaatt
      121 gatttcatta aatcttcaaa ttcattaaag caaagagatt gagcaccatc tgacaaagca
      181 ttttctgggt caggatgtac ttctaccatt attccatctg ctccacatgc aaaagatgcc
      241 agtgacatag gttttaccaa atatctaact ccagtggcat ggcttgggtc taccacaaca
      301 ggaagacctg tttccttttg aataatgggt actgcagcta aatctaatgt atttctagta
      361 taatcattat acgttcttat acctctctca cacattataa tattactatt tccttctatt
      421 gctatatatt cagaagcacc tatccattca gtaattgtgg atgctattcc cctttttaac
      481 ataacaggtt tattttgttt tcccacttca cttaaaagag taaagttttg catatttctt
      541 gacccaattt gaattatatc acttatacta tacagttcgt ccatatctct tacatccata
      601 agttctgtaa taacagccat accaacttcg tctttaaccg cttttaatat ttcaagacct
      661 tcttttttta atccttgaaa tgaatttggc gatgttcttg gcttgtatgc tccacctctt
      721 aaaatgtttg ctccctgact ttttacaaat ttagcagtct ccaaaagttg ctcataactc
      781 tctattgcgc atggtccagc tattataagt ttatcttttt ttacagttat ctttttgtta
      841 tttgtattta tctctatctt tgaattatta ttaaataggt tttctttcat aacctacaag
      901 ctcctttact tcttctcttt cttcagcaga taggaggttt aaatgttctt ttattgtttt
      961 ttcatttatt ataagttttt catttaaatc cataagtggt atcaaaacga aagctctatc
     1021 ttgaatcctt ggatgaggta ttgttagtct ttcatcagtg gaaactacat cattaaaaaa
     1081 tagtatgtct acatctatag ttcttggtcc ccaatgaact attctctctc tgtgtagttc
     1141 tctctctatt tcttgacaat attctaacaa ctcataaggt tcaaactctg tttctatttc
     1201 tacacacata tttagaaaat ctgcttgttc tgtatatccc catggttttg tttcgtataa
     1261 gcttgattct tttactttat atatagaatc actatttttc aaaagttcac atgctcttga
     1321 taaattatca aatctatcac ccatgttagt acctattccc aaataagcct tattcatctc
     1381 ttgttctcct tatctcaaca ccaaaatagt cataaatacc atttacagga gcctcaggct
     1441 ttctaactct gaccataact ccatttataa gtataaattt attcaagact tcttcagcaa
     1501 tattttccgc taaagcctcc aaaagattaa attgtttatt ttcagtaata tctttaacaa
     1561 cattgtaaac ctcggcatag cttacagatt tatttatatc gtcactaaga cctgcttctc
     1621 ttgaatcaat ataaagttcc atgtcgacaa agaatttctg acctagaaaa ttttcttctt
     1681 taagaacacc atgatatcca tagaatccta gattactaag taatatttta tccattatat
     1741 tatacctact ttctatatat tgcatcagtc attctagctg ccattaaatt ttcataaaca
     1801 tcatgtactc taactatatc tacgccatct cttataccaa gaacagtagt agctattgta
     1861 ccttctaatc tttttttagg ctcaactgga agtacttttc ctattactga ttttctggat
     1921 gtcccaagca aaataggata ccctaaatct tttaattcat ttaatcttct caatacttca
     1981 atattttgtt caaaatcctt gccaaatcct acacctgggt caagtactat tttttctttt
     2041 tttacaccac aactcattgc catttctata cttaccttaa aaaagtcttt tattgactcc
     2101 attatatctt tgtcatagtt tgtcccatct tgattatgca ttatacacac ttctgcatca
     2161 tattttccta ttacagaagc catattttta tcttttctaa gaccccaaac atcatttacc
     2221 atagtgacac ctaattttaa tgcttcttct gcaacatctg ccttataagt gtctattgat
     2281 attggtatat ccaattcctg tttaagtttt tttattacag ggattactct tcttagctct
     2341 tcgtctgagt ctacatagct atgacctggg cgtgtagact ctcctcctag gtctattata
     2401 tctgcacctt ggtcaaccat atcttttgca tgttgtattg ctatgtccaa gttattaaaa
     2461 tctcctccat cagaaaaact atctggagtt acatttaata ttcccattat ataagttctt
     2521 ttaccataat caaacacaat taatgacact cccttaaatt aaattttatc tcaataaatg
     2581 attatatcta taataaattg ctacttcata gtaatcaaac tcatagcctc ggctctagac
     2641 gcaatgtcct tttcaaatat tcctctcact gctgatgtaa cagtttttga acctggtttt
     2701 tttacgcctc tcattgtcat gcacatatgt tcagcttcta ctactactaa gacaccataa
     2761 ggttgtaatt cttccattat tatatctgca gcatttttag tgattctttc ttgtaattgt
     2821 ggtctttttg ccaatgtatc tattactcta gcaagtttag aaagccctgt aagtcttcct
     2881 ccttttggta aataagcaat atgagctttt ccaaagaaag gaactagatg atgctcacaa
     2941 catgaataaa atggaatatc ttttaccaat actaactctt catgtttttc atctgcaaaa
     3001 agaactttta aatggtctct tggttcttca ctaagaccag agaatatctc ttcatacatc
     3061 ctagcaactc tatttggagt ttcgattaga ccttctctat ctgggtcttc cccaattgcc
     3121 tctagtattt ctcttactgc atgttgtatc ttttctttat caactttatt catttaaact
     3181 ccccccattg atttgtgact agaagattag ttttataacc tcttttgtct caatttcttt
     3241 aaaaatataa ttataaacat ctaaaagtct ttgatagtca taattttcac ttacttttga
     3301 cactagttca aaaagctcat tattttcttt ttttaaaact tttagcaatt ttttgtcttt
     3361 tggtacccat ttatcattta tattaaagta cccaattatt atatctttta aatataaatt
     3421 cgtcaaaaaa tgatattcaa aaacatcaaa tttttcttta ttttttaaat tcgatatttt
     3481 tgctccaata tcttgcttta taaaagactt ttcctctaat gacattttac taggtccttc
     3541 taagtaaata tctctactta tatcttttat aatatgtgat atattctccc tatcaaacag
     3601 tactttagca tctttcattt ctttcacaaa gaaatattcc ctatttgata ataatttttt
     3661 tacaccatct tttgaaaagt aatttacatc aaattctatt cccttttttt taaacagtat
     3721 tctctcttgt ttttcccctt cttttacaaa aacgaacaca tcaatatcgt ttatcactac
     3781 atcataaaga gtaaaatcta catctttact agaacccact aaatatacag ccaaagtgtt
     3841 ttggttttct attattttag tcatctcttc attgtatact tcataaatac aacctatcat
     3901 cttttgtctc caacaaattg ttaatatttt aatttattct aataacttca ctattttatc
     3961 aaaaacaaca ttttctttat taaattttat tttgtcaatt tctgtaacct ttatagcgcc
     4021 cataagacta tttgtgacaa aaacaaaatc aaaactacta atttctgata tattaatttc
     4081 attttctatc aactctatat gtaattcgtc acaaatctct ataatccttt tttttattat
     4141 tccatttaat atcggtaatc tatcactagg agtgtacact ctttctcctt ttataaagaa
     4201 aatattgctc atagaacatt ctaatattac accttctgaa tttaaaaata taccatcatt
     4261 ataaccagtc ttatttgcga tatttttggt atatatgttt tcaaaataat tagtagtctt
     4321 atgtctatga attaaactat tccccctaac tattggagat atagctaatt taaacccctt
     4381 ctcatacgtc tcttgattat atggaatatc cctaatagat atattataac cttcatcaaa
     4441 aacagtaatt ctaagtgctt tattaatgac attttctttt tttatatatg taacaatttc
     4501 atttatcaag aaatcttttg tatagtttat atctaaatcc aaacaattta ttgaacttag
     4561 cattctttct atatggatat ctaaatctac tgctattcca tttttaactc taatagtttc
     4621 aaacaaacca attccaaatt tacttaaact actatcaaaa cctacattat tcttcataat
     4681 atcccctcaa agccttcatt atggatttag ctttatcaag tgtttcttga tattcttcat
     4741 ctgggtcaga atcccaagtc attcctcctc caacttgaaa gtagactttt ttgtcatttt
     4801 tgattattgt tctaattgct atattaaaat ccatatcacc attaaaacct atataaccta
     4861 ttgaaccagt atatacattt ctttgcgttg gctctaattc gtctataatt tccattgctc
     4921 taatcttagg tgctcctgtt atagaccccc ctggaaaagt tgcttttatt acatcagtaa
     4981 catctttatc atcttttaac tcacctacaa ctgtggaaac taagtgattt acatttgcat
     5041 atggttcaat tacaaatagc tctggaactt taacacttcc agttttagat attctgccaa
     5101 tatcatttct ttctaaatct actatcataa gtagttcagc tctatctttt tcactatttc
     5161 taagttcttg ttgaagtctt aaatcttctt ctttatcttt ccctcttgga cgagtacctt
     5221 ttattggtct agtttctatt ctcttattaa cacatcttat aaatctttct ggagaatttg
     5281 ataatatatg tgtatcttca aagtttaaga atgccccaaa tggtgctggg ctaaatcttc
     5341 ttaagtccct atacaattca aagcttgtaa gttctgtttc tccactgaac ctttgtgtta
     5401 aattagcttg atatatatct ccttgtctta tgtactctct aacactttga actgcatttt
     5461 taaattcttc ctttgtaaaa ttagatttaa gtcttatagg tgttacttct ttttcttcat
     5521 aacatatgct atctatacct tttttctctt cttttaatat tctttgttct atatcatcta
     5581 ttatcttttc ttctaattcg atatctatgt ttggagtagc tatataagtt ttttgcacaa
     5641 gatggtctat aactattaca tgattataaa atccaaaata catatcaggc atctcaatat
     5701 catctacagc agttctaggt aaattttcta tgtaattacc taaatcataa gataaatatc
     5761 ccacagcacc tcctacaaat ggaagattag tatcatttac aaccctatac ttatgtagtt
     5821 cttcttttaa aacttctaaa ggattttcat cagtatcttt atactttaat accttaaatg
     5881 gttggctact tataaaagaa tatcttccca atttttcttt atccatagca ctgtctaata
     5941 taaaactgtc atgttcattt ctaaaaatag taaatatctc aaaagaattt aatttagtat
     6001 ttatctctct tatcatatta catatgtacc ctttctctag cttctgttat aaaattttta
     6061 agcatttcat gaccatactc tgacaaaata gcttctggat gaaactgtac tccttctatt
     6121 aaatatttct tatgcctaat tcccattatt tcccctttat cagtaattgc tgtaatttct
     6181 aattcttttg gaactgtatt ggagtcaact attagtgaat gatacctagt gacatttaaa
     6241 ggatttttaa gtcccctaaa aactccttta ttagtatggt tgatactata tacttttcca
     6301 tgcacaggct gttgtgcttt tataatatct cctccaaaaa catgtccaat agtttggtga
     6361 cctaaacaaa ttcctagtat aggttttttc cccttaaaat gctcaacaat atctatgcaa
     6421 ataccagctt cttttggaga acacggtcct ggagataaca caattacctc tggatttagt
     6481 tcttcaatgt cacttatttt gatttcatta tttcttttta ctacaactgc ttcccctaat
     6541 tcttctatat attggactag gttgtataca aaagaatcat aattgtctat cataagaatc
     6601 atactgtaaa tctcccccct aaaaattatt ttatcaactc agaaactgtt acaaatttaa
     6661 aacctttttc aatcattttt ggaataaaca tatctaaagc ttcaactgta tcattagttg
     6721 cataatcatg caaaagtatt atatctccat tttgtacttt attaattatt ttatctgcaa
     6781 tgctattagc tcctggattt ttccaatctt tcacatctac acctgtccat aaaacaactt
     6841 tatagtcatg cttttttgca atttctatta attctttttc tctataactt ccaaatggtg
     6901 gtctaaataa tgtcggtttt tttccagtta cttccttcaa aatgtcttca cacttaacaa
     6961 tctcctcttc tatctgacta gaacttaaat tacttatatc aggatgattg aaagtatgat
     7021 ttcctatctc gtgaccttct ttacttgctc ttactaatgg ttctttatac cattttgcat
     7081 gttttccagc tataaaaaaa gtagctttta cattgtattt ctttaataca tctaaaactt
     7141 gactagtttc tttaggatga ggaccatcat caaaggtaat tgctattagc tttttttccc
     7201 ttgaaccatg tttaatcaaa acatttccat tagtatccat catcaaagca gaaactttac
     7261 taaaattaga tattaacact attccacaaa acaagactac tgctattaag cacttatata
     7321 ctgtcttttt tttccatgat gtcatttttt aacttccttt caaatatttt agttatttat
     7381 aaaaattttg agtgtaatat gttttatagc ttcctccaaa tattactcca actccaatat
     7441 tattatagtt ccccaaaata ttttttctat gtccttcaga gttcatccaa gcttcatgcg
     7501 catatattgc attaatctgt cctgctgcta tgttttctcc agctgaagta tatactatac
     7561 cttctctttt cattctgtca aatggagttt cattcttctt atttacatgg tcaaaaaaat
     7621 tattatctct catatcttca ctatgttttc ttgaactcaa agttgcctgt tcactgtatc
     7681 tcaatgagtt taaccctctt tgatttctaa cactatttac aaggtctata acttcaagtt
     7741 caaaacattt tttaagttct tcgctatcat ctggatataa atttttaaac tcatcttcag
     7801 tgcttttatc aattattaaa tatgaacaaa ctctattttc ttcatgaata tcgtagaaaa
     7861 ttgtaatata cttaccttcc ttacttatta tatcatattg attttctgag tttattatat
     7921 atctggtatt tccttttctc ttatatttta atggtgtaat cttagttctc actgtttgtc
     7981 tatcttgatt tagttttata tcttgatttt cacatgaatt aatagaatta ctataaagtg
     8041 ctactacttc atcattttca agtccaacca ttgcaaattc ttttccatat tgattatata
     8101 cataccaatc aaaaccatat tcactaatat caattctacc aggtttgccg atacttgata
     8161 aaacatagct taaattatct cctaagctta ttttttcaaa attctttata tctgactctc
     8221 ctaagttctc cccttcagta ttatttgatt gtaaattttg acttttttct tttttatctt
     8281 tcttagaatt atcatttata tttattttgt ctaaaaattc attccatatt tcactttgaa
     8341 aaaacttttc ttttaaatca tatccttctt ttaataattc aggactataa aataaaacaa
     8401 taagtactac taacactaaa gcaattaatc tcttcaatat atctctcctt cttaaaatca
     8461 atcaaaattt tttaatattg catttattaa gttaatattg atagtgtata ctaatcaata
     8521 tcattcatac ctatttacag taagtattag aaaaaagctt tatctactaa ttagataaag
     8581 ctacagtctc aaggaaaata tttcatctaa tgttctatca aatatagatg gcttatcaaa
     8641 tttccaagtc ccttcataaa ccaattcatt atttagataa aaagttcctt gcccatgttt
     8701 tttaccatgc ataaattcac ctatatactt tttacctttt tcccatatat aagttccctt
     8761 accatgaatc aaattattct tccattctcc ttcataaagt acttcccctt tactattata
     8821 gcaaataccg tacccttcca tctttccacc tttaaattca cctgcatata tacatccatc
     8881 actccactta taaatacctt ttccatcttt ttttccgttt ttccaagttc caatatattc
     8941 atctttacta ctatataaca tatttccaat accgctcatc atatcttcct caaaataacc
     9001 tatatattca tttccatttg aaaatataaa gtttcctttt ccattttttt tatcttcttt
     9061 aaattctcct atgtactcca taactgttcc ttcatctaca aaaaaagtat atgagccttt
     9121 cccctccatc tttccctcta agaatttccc tacatataca tccccatttg aaaatttata
     9181 gattttattt tttggttttt ttgtatcgac atcatctata tcgcgtattg tttttccttc
     9241 caattctaat acttcatttt ccggagcaaa atcgcccctt tttaaaacac gtttcttcat
     9301 gttctttata cttctaatct ctttgtttac ttcatttata tatttcaaat aattatcata
     9361 caaattaaca tattctttag ataaattcat aatgccattc ctccaacgtt attcctagat
     9421 attttactaa atctattaaa tttattatat cctaatagtg gtcatgtttt ctacatatta
     9481 agtaattttt tcaattttaa ttttaaaatg atatctttta aagtgataca caaatcatta
     9541 ataaaacctt tattttcgta tactaaattt aaataagctg agagatataa tatacaaatt
     9601 tacattataa actcccagct tgtaaaattt tctataaatc ttttgcttta aaccctcttc
     9661 cctttacttc tgtaacatca tttacagata taaaggcctt tttatcatac ttcaaagcta
     9721 tatcttttat ctttggtatc tcatttgatg aaactattgt atatataatc ttcttttttt
     9781 gatgagtgta tgcaccttct gcatctaaaa aagttacacc tctaactaaa ctcttcatta
     9841 tatcttctgc tattaaatcg acttcatttg acattaccat aacagatttt tggtaattca
     9901 ttgcatcttt aataaaactc attgaatacg catttagaaa tagacctata agagtgtata
     9961 atgctaaatc caacccaaat aaaaaacttc ctgtaagtac tatcaaacca tttattgcta
    10021 aagcagtgtc tttcatttct atattcttct taatttttag tattgcagct attatatctg
    10081 taccaccttg agaagaccta gttctaaata caactccgac tcctaaacca gataatatac
    10141 caccatatac actttgaagc aatatatcat gtataggtat aatattactt atttcctgag
    10201 ttgctcctaa taataatgaa aatacaatca tatttaccaa acttgaaata caaaactctt
    10261 tttcaaggta tatgaaccct aagataaata ttggaatatt gattaaaaat gttaataaac
    10321 ctacatttat atttgtcaaa tagtttatag atgttgctat acctgtagca cctccactaa
    10381 gcagatgtgc tggtcttaag aacccattta ttcctataga tgtcatcaac atcccaaata
    10441 gcattgttga atatcttaaa actggatgtt tttgtagggt attcttaact tcactcattt
    10501 ctttctccct tcaatctttc taaatcttta gaattttaaa agcttactct tacatatatt
    10561 attcctgatg taatagataa tatttcaaat aaattttttt tataatttct tgaactgtca
    10621 tctattacat catctattat attatctttt tcatatatgt ctagtgcaat cttttatatt
    10681 ctaatcttta aaaacgttca aatcatctca tttatgcctt aaatgaaact ttttctagca
    10741 taatatgata ctgcttatta tttaacttgc aatatagtaa atttatagta tttttttcta
    10801 aatatctatt ttcgcagtaa tttattttat tttagcaaat tcaagtttag taattgtctc
    10861 tttataatta attcctaaat gaaatttttc tttttataat ttcagtacat gattttttta
    10921 tttcattatt tgcatttttt tcgttcagtt tttattttaa atttttttta ttttattttg
    10981 ttttaagtat tcaaggtact catcttaatt ttatttttta tataaaattc attataattg
    11041 gaattagtat tactgtaatt gtaccagaaa ctccaataga aacaccactc atagctcctt
    11101 ctatttctcc aagttcaaaa gcctttgtag ttcctaaagc atgtgcagat gttccaagag
    11161 caattccttt agcaactgga tgatttatat tccccagttt gaatactgta gaagcaacca
    11221 taccaccaaa aatacctgta aaaattatgg ctacaactgt tattgattct ataccattta
    11281 aagaatttgc taaagatatt cccattggtg ttgttataga ttttggaatt agtgaattta
    11341 ttatacttac atctgaatta gctattttgc caattatcaa tacagataca aaagaaatca
    11401 ctataccaca acctattcct acaataatct caaacatatg ctttttaaat aattcaaatt
    11461 gtttataaag tggaactgca agtactactg tgacaggacc taaaaagaaa tttagactat
    11521 cagctcctat cttgtaattc tcataaggga ttttagatat gcttaaaaac aatattattc
    11581 ctacgattgc aattaataaa gggttacata taggattact tgtttttcgt tgaatgtatc
    11641 ttcctaaatt gaaaaaaact attgttacta ttataccaaa tactggtgtc tgtaatacat
    11701 tatacatttt tttgtccttc ttccttcttt tttatcattt tctccacaat aattccagta
    11761 gtatacatta ctattagtgt acttaaacat atgactaata aaagcactaa tacattttcc
    11821 cttattaggt ctaatgactt tataagtgat acacctgctg gaataaaaaa tatagccata
    11881 ttatcgagga aaaatccact tacattttct atactactca agtctaaaac tttaaattgt
    11941 agtagtagaa ataataatag cataccaact atactcccag gaataagtat aattccttgt
    12001 ataaaggaac ttatatactc tcctattacc cagatagata aaattattac taattgattg
    12061 aaaactttca ttttccacct ccaaaaatta aagagattgc ttcaaatcat atatttgtag
    12121 ttttatcaac aagcaaatat ctttctttaa aatagaaagt tataatttga cacaatctct
    12181 ttttaaattt atttttattt taatttaata aaatacatac ttggttgttt caaaaaactt
    12241 aactatataa acctattttt cacttaaaat ttcttttatt tttgaattgt tatacatact
    12301 gtctggaact tcacttctca aactataaaa agtctgttgt gcatttttta aatcgccatt
    12361 atcataataa agcataccca actcgtaata tgaatcatca taatactggt catttttagt
    12421 ataagtattt atatattttt tgtaatattt tatagcttca tccttatttc ctaataattt
    12481 atttgttatt gctaattgat atattgactc agaaatatat tttttagttt taccagaaga
    12541 aactatactt ttaaaattat ctatggcttt ttcatattct ttgtttttct ttaaatttag
    12601 agcttgataa tataaatctt cctcatcagc tacagttatc tctctttctg gttctttatt
    12661 tttaacctca tttaattcct tattcgtttt ttctaatttt ttactagttt ctcctaactt
    12721 tttatcagtt tcacttaact tttcctcttt actacttatt tggttattca agttttctat
    12781 tctctttttg ctttgaatca aatatatccc catacctatt aaaacacagc caactataat
    12841 atatggtaaa atacttttct ttctttttac tttttttatt tcttcatttt tacttgtatc
    12901 ttctttttct tcttcatttt tttcaaatac aggattatct ttatctagct ctttaagttt
    12961 gtctaaatat ccttctctct tttttacatt acctagttta tcatataaca aagataaaat
    13021 tacatatggc tctattagtt caggttcttc ttcaattatg ttctctaaaa tatgtattga
    13081 ctcataattt aattcttcat ttataaatct tatagaatga ttgtatcttc ctaaaaaagt
    13141 tttaaaatct tcagatgaaa ttagatttac atattttcta gatatatgat tattctcata
    13201 acataaagca gtataaaaac tctcaaatga tttagcaaaa tcacatttta ataattttaa
    13261 aagacctcta agatttagta tatcaatgtc tttaggattt atctccagag ctttattaac
    13321 catattaatg gcactagtta tataattttt atatgctaaa tccaatgcat tgttatatct
    13381 tttatatgat tctaatttta actccttaga cgttgtgtct gactttgaat ttaaagctcc
    13441 ttgcaagttt aatagagttt cttctttata atttgtagtc acataactct gttttaatgc
    13501 ttttaatgtt tcatcaccgt cttcttcttc tatattaaaa aaagtcaaat cacttttatt
    13561 atcttctatc aaagatatat ctaaaatttt tctaggcatg ctttctatgt cttgttttat
    13621 atctttaact aaaataatac tcacctccac tctaaaatat atatttaaga taacatattt
    13681 aatatgtcaa gtcaaaacta tacatatttt tccaccattc tcataataaa aaaggtaatc
    13741 ctcttctttt cataaaaagg ttacctcttt ttcaatacta ttaactttta atgttgaatt
    13801 tttttacttt tctatagagt gtagctatac ctatatttaa tgcctcagct acttttttca
    13861 taccttctgt attacttcca tatttcttta tggcattttc tatagcttcc ctctcaaggt
    13921 catctatagt cttaacttct tgtttataaa gttcatctag tttaaaagat attaaatcag
    13981 acatactcct tacaaaagca gttaactcct tatcttttga aagtatcttt tctttttgtt
    14041 cttcattaaa cgcagctata cccaagattc caagtatatg ctctcccatt ttaacgggag
    14101 tacatatatc agctaactct ttacaatttt ctctatttgc acatttatta cagttctctt
    14161 ctatttcttt atttataaca taggattttc catcttttaa aacaagagag tacactgact
    14221 tctctgatat cgtctctcct attttacctc tgaatattcc tgtacctgct atcctagtta
    14281 aatcttttgt aactaaagta acatctacat ccaagacatt agaaatattc tcgcaaatac
    14341 tttgaacgta attcgttata ttatttaaat tcaaagtaat ccctcctatt tagatttata
    14401 ttttttatta ctctacataa atcctattct tgagaacttt aagcttgcgg ttgtggttgt
    14461 gtctcattag gcttattatc agttggtggt gtttctggtt tttgttgatt gtttggattt
    14521 tgattgtttg tattttgatt attcttattt tcgtctgtat ttggtttttc ctctggtttt
    14581 ggttccatag tcccaactaa tataatacct tccttcttag gataatatga attagctatt
    14641 ttttcagttt taattttttt accatttgca tcattaaatg ttctatatgt tgatacagaa
    14701 tatgcatttc ttccactttt gtctactttt tctttacctt taggcattgt agggtcatta
    14761 actcttttca ttgtagtttg tgaaacgcca tctaattttg ttgatatcgt tatattttgt
    14821 ttatcttctg cacttccata tatattgcaa acaatttgat ttcctgaaac gtaatttttt
    14881 atgtaaactg gatgctttag attattttta aataagaaat ctattccact atctgcaaca
    14941 gttgcatctc ttccttttgg aacataactt gatggtatag tatgattttt tacattaaca
    15001 atttctaaac ctgcgtatag cacagaattg tataacgtag atgaaacttg acaaactcct
    15061 ccaccataat cttcttccat ctcaccttcc attattacag gtgcactttt atatccattt
    15121 tccttacttc ttttccctgt atgtttatta aatgagaaag tttctcctgg cataagtaat
    15181 acattactta tattatctgt tgctaatttt atgtttatac ttctatttat ttgagaagtt
    15241 ttaaatgttg ttgaatacgt acctaaattt gtatctatat tagcaagttg ctcttttttt
    15301 acttttgctg gagtagcttt tactactaac tcatttttat agttaccttt tttagtttga
    15361 ttatcaaaat tctccatact cttagaaata tccattttaa gaccatcaga atctggtata
    15421 accttgactt tttcaccact tatatcaagt gtagcatctt ttacatctac atctatttct
    15481 ttagctatct tttccatttc tgcttttaat ttatttttat cataatttat cacaactact
    15541 aagttgcttt ttctgccaaa atttgctgaa atagtttttg atagattgcc aaagaaacca
    15601 ctctttctat ttaaattata tgcgttttct actgttttat ccaggtcagt agataaattt
    15661 aaatctttgc tctttacttt ccaactttta tcattgtaat taaaatcaac attctctaat
    15721 ttgtattttt tagctaattc ctgttttgct tgcgatttag ttaattttcc aatatttact
    15781 ccattaacat atgtgttttt tgctattttc tcccctttaa attgcatgca cattatgcct
    15841 ataaaaatta ttactactaa tgctacagct ccaatagcta taactagctt tttgttcaca
    15901 ttactcatcc catacttctc cttacaataa aattatttat atatccctat tattatatta
    15961 ctacataatg cgccttaaaa ccccaaaaac tttttaatta tatattaatt taatattatt
    16021 aattaatatt catttagtcc actttctgat atggcatata ttttttcatt gtaaatatta
    16081 atagcataaa cttctttgcc tctaaataag cctttattta ctacagcata ataataaata
    16141 tttttatatt ttccaaacaa ttcttctaaa ctctttttac tttttacata tctatctgct
    16201 ttctctatat ctaatatctc aatcttttta tacttatttt ctttttcatc aagagtatta
    16261 gttaacacaa acttagcttt atcttttgta ataccaaatt gataacttcc atcttctaat
    16321 attctaaagt atccatcacc ataatctact ttgtaatctt ttttaccatc tttatatgaa
    16381 tataaatctt tagtttttgc acttaccaaa acttcattta ttaatttatc atactcatat
    16441 acttcagcat aatagtattt attatttttt atcatttgtg gaagtgagtt tatagataga
    16501 tttgccttgt ctttgccact ttttttagct attacattta taatgtcatc agatgttaaa
    16561 gtatacaatg ataaattctt tttatatttt ttttctaaac tctgtacctt ttcatcatct
    16621 gaataatcgt cttttaaata ctcaatatat tgtaatgctc tttcataatc tccagaagta
    16681 tttgcctctt cagctctact tatatagtaa tcatacattt cttcaataca ttgttctttc
    16741 ccattttgag ccatactgta atatttttca tcttctttta atactttatt atatttttca
    16801 atagcttcat aatacttaca tgctttttgg tcttttactg ctgagtcata aacatctcta
    16861 gactctttga ttgtttctat attctgtttg tatacatcta ggttactacc cattccattt
    16921 aatattgaaa cagtacttat gtagttaatg gcaatatcat aagtagtatt ttcttcttta
    16981 tacatttctc taactctatc agcttgaact aataagtcat ccacatctat acttattttg
    17041 tttaattctt tcacggtatt tattaatcca ataaaacttt cttgacttat gtctccatct
    17101 aaatatttat caccactatt taatagcaat ttatttattt ttttagaaac agacttatta
    17161 aacctttcca tttttgaatc agaaaacttt ttttctatac ttgtgtagta atcaacagct
    17221 atagaatact gtcttttctc tataatcttt aacaactcat tagcttcatt tcctacacta
    17281 aactcgttac tacttaagaa aaatactatt atcaaagcaa ttacacctaa tgttgataat
    17341 gaaatcgcaa ctatagattt ccttttttca tcaataaaat ctacagcctt ctcaaataaa
    17401 tctctaaaat acattatagt tccctctcca tttaataatc tttatatttt atggtaaact
    17461 aaatttctta ttggttatta tatcataatt cttgaaaaaa aataaaaaca caagttaaat
    17521 cgtgttttta ttttacctat caatcattat tttatacata aatatttcct ttacataaat
    17581 tatgatatct ttctttttat atccctagaa tgactctttt gttttttatt agtactgaca
    17641 gttttcgttc ttgtcttgtg tatagctttg ctatcaaata tttttctagc attgcataaa
    17701 acaattccaa ttgttaaaca aactgtaagc acacttccca tcaaaaagta ataatccaat
    17761 cctatatgta ctcccataac tgaatttatt aacactaatg caaaagtcat tgcaaaacat
    17821 atcatcatgg agataactaa aattcttaga atttttttca atccactcac ctcgtataat
    17881 cactaaaatt gacactaccc cttatccatt actataccaa attagtaaaa aaatagcaat
    17941 tactattgtc atttatatta ggaaaatatt aaatcttttt aataaatatc tttttttgac
    18001 ataaggatta cttaactttt aataaaatag taacactaat atgtattaat taatagaata
    18061 aaataaatga taatttattg aggggtgatt tacatgccaa atttgccaag tttagggtca
    18121 aaggctcctg attttaaagc caatacaaca aatggtccta ttagactctc tgactataag
    18181 ggtaattgga ttgttttatt ttcacatcct ggtgatttta caccagtttg tactacagaa
    18241 tttttatgtt ttgctaaata ttatgacgaa tttaaaaaaa gaaatacaga actaattggt
    18301 ctaagtgttg atagtaacag ttcacattta gcttggatgt ataatatttc tttacttaca
    18361 ggtgtagaaa ttccatttcc tattatagaa gatagagata tgagaattgc caagttatac
    18421 ggcatgatat caaaaccaat gagtgataca tcaactgttc gctctgtatt tattatagat
    18481 aataatcaaa ttctaagaac gattctttat tatccactaa ctacaggaag aaatattcca
    18541 gaaatactta gaatagtaga tgcacttcag actagtgata gagataatat agttactcct
    18601 gcaaactggt ttcctggaat gccagtaatt ttaccttatc ctaaaaacta taaggaatta
    18661 aaaaatagag ttaacagttg taataagaaa tattcatgta tggactggta cttatgtttt
    18721 gtaccagata attataatga tgaagaagtg agcaagaaaa ttgataatac ttgtagctgg
    18781 aaaaaagaac atactaaaaa cattgaaaat gaatgtaact gtgaacatga acatcatgac
    18841 tacctgaaca aagctcttga ttgtaaacaa gaacacaaga ctgatattaa agatgattgc
    18901 aaccatgaga aaaaacatac taaaaatact aacaaagttc acaactccaa acaagataag
    18961 tttaaagata agtcttgtga tgaaatgaat tttaactatg ataaagatga atcttgcgac
    19021 aaaataaatt ctagctatga taaagaagat agtagttatg aagatttcta taaacataat
    19081 tataaaaact acgattatac tagcgaaaaa aatactaaaa aaatagctat gaaaacttta
    19141 aaagattcaa aaaaattagt tagaccacaa ataacagacc catacaatcc aatagttgaa
    19201 aatgcaaact gtccagatat aaatccaatt gtagcagaat atgttcttgg aaatccaact
    19261 aatgtagatg ctcaactatt agatgcagtt atatttgctt ttgctgagat agaccagtct
    19321 ggaaatttgt ttattcctta tcctagattt ttaaatcaat tacttgctct taaaggtgaa
    19381 aaacctagct taaaagtaat tgtagctatt ggaggttggg gagctgaagg attctctgat
    19441 gcagcattaa cacctacatc tagatataat tttgcaagac aggtcaatca gatgataaat
    19501 gaatatgctt tagatggaat agatatagac tgggaatatc ctggaagtag tgcatctgga
    19561 ataacatcaa gacctcaaga tagagaaaac tttacactct tactaactgc cataagagat
    19621 gttataggag atgataaatg gcttagtgta gctggaacag gagatagagg atatataaat
    19681 tcaagtgctg aaatagataa aatagctcct ataatagatt attttaatct tatgagttat
    19741 gattttacag caggtgaaac aggtccaaat ggtagaaaac atcaagcaaa tctttttgat
    19801 tcagacttat ctttgccagg atatagtgtt gatgcaatgg taagaaatct tgagaatgct
    19861 ggaatgcctt ctgaaaaaat ccttcttggt ataccatttt atggaagatt aggtgctact
    19921 ataacaagaa cttatgatga gcttagaagg gattatataa ataaaaatgg atatgaatat
    19981 agatttgata atactgctca agttccatat ttagttaagg atggagattt tgcaatgtca
    20041 tatgatgatg ctttatcaat attcttaaaa actcaatatg ttcttagaaa ttgtctaggt
    20101 ggtgtattct catggacatc aacttatgac caagcaaata tactggctag aaccatgtct
    20161 attggtataa atgaccctga agtattaaaa gaagaacttg aaggtattta tgggcaattc
    20221 taatagaatg taggaaaatt ttatagttat ataaaaagca gattttatta tattaaataa
    20281 gttgtatcta attaatacta ttattaattg tttataaatt agatacttat tttttcataa
    20341 tacaatctgc ttttttaatc aaaatttatt taattataag ttgtttaatt acaagttgtt
    20401 taattataaa gtaggaattt agatatactc ttataccatg taattatgta taaatatttt
    20461 tttattatac accaatagct tttttagcta atttatcagc ttcctcatta tacttatttc
    20521 cagaatgagc tttaaccttt gtaaatttta cattaagctt attcttaata ctgtcataga
    20581 aatttttata tgctatagtt ccttctttat ttgttttcca aactccagta caccactttt
    20641 caatcccttc atagtcaaag tataaaatca agttttgaac attattgtca atacagtact
    20701 gcattgcaat tttagaagcc tctatctctc cagctacatt tctcatactt actaaagttt
    20761 tctcttttcc tttttcacta taagttttta taacttcatt attttttaaa atcacaacac
    20821 ccgaaccata catttttact gaatgttcat aacttccatc tacatatgct tctataaact
    20881 ccttatcttc ttgaaaactc aatttttctg acccatcaat atattcttta gcttcttgaa
    20941 aagtagaaaa actcttatat tcagctcctg agaatccatt tacttgtttt ttgcactcat
    21001 cccatgtatt atatacacct atatttttac cttttttcac agcataaaac ttttgttttg
    21061 ccaaatatat cactcccact cttaattttt atcttcttct ctcataaata tatctaaatt
    21121 tataagatag tttctatcaa aatattttac tttcatatct ttttttataa tatcaatatg
    21181 gtacacttct ccttttacag ctctttgtct tatattttca tttatatctt tttttataat
    21241 atccttatca acatcattgt tatccatttt agcatcaaca ttcaacgctt ttatagtatc
    21301 aaatataaca gattttagta ttaaacttct agatttattt aaatgtcgcc ttatataagt
    21361 ataaaactca ttgctattaa cctttgaaat tttacaatcg tgtaaatctt tctctaatct
    21421 ctttaaaaca cttatattag ttcctacatc aaaaatcaca aaacatccat agtcatattt
    21481 gcttcttaaa attctcccca tagcttgctt tacctttatg gtcatttgag gataatttat
    21541 agtatagtag tctataccat acttcttcat tatagtaaaa tacaaagggt cttttggatt
    21601 tagattcgga agcttgtcta aagttacaca aaccaaacca tcccctggaa tgtctacgcc
    21661 ttcaaaacat ccttttgagc caagtactac acattttcta tttaaatcat taaggtgtct
    21721 aatgcccttt ttatcagcat aaatttctaa gttaaaacta tgtaagtatt ttttcaatat
    21781 ttcataagtt ttttcttgtc tatctttact attaaaaaga gctagtgtat gaccttctgt
    21841 aatctttgat atattagata taattttact catctcattt ggaaattcag aatttctgta
    21901 ctcacaaata tcagaaaatc caactacaga gactctattt ttataatcaa aaattggttc
    21961 tataatcttt tccacattct taactctatc tattcctaat gtatttttaa aataagacat
    22021 actttcagat aaactcaaag ttgctgataa aaatattcct ttttctaatt gagataaaat
    22081 attttcttca aataaatctg ctatttttaa tggtactact cgaaattcaa agtcattata
    22141 attcttatca atttcaacta tccttgcata atcatccttt tcatcatact ctaaaaagat
    22201 ttcaaatata atctttatat cctctaaatc tcttgtcttt gcctttccaa atttatatat
    22261 atctgcttct ttatctatac tatcatcatc aatattccta taaattatta ttattattga
    22321 aactaaattt tttattattt tctcacaact caactttatc ttttcactat aggctctata
    22381 acttatttca gtatctatac catccttttt tagttttccc acaatttcat ctatttgcaa
    22441 attcagttcc catcttaaat tataattact tacattatta tattcattac aattaccaaa
    22501 atttaatatt gaatctatct cttcaataat gagatttatg cttctagcaa ttttttgttt
    22561 atcttctctt ccaattttta atacattata aaatttatca aaagccttta tttttctcat
    22621 atttctacta tattttttat atataaagct actattttga ataaactcgt aaggataaat
    22681 ttcttgtaat aagtacctta aagactttga atttataata cttgaaaaga aatcatatcc
    22741 tttttcagtc aaattatgtg cttcatctac tattatattt tctaaaggtt tttcatcctt
    22801 atacggccat ttagctaata gtgaatgatt tactactgtt atatgttctt cttttaattc
    22861 ttctattctg tttttatata aacaatcttt tttgcatttt ttaggtctgc acatatttgg
    22921 gtcacaactt acatttctta agtgagtact tatttccttg aagttgtcaa gcactaaata
    22981 atttatttct tctatatctc catacttgcc accttcaact aatcttttca aaaatattaa
    23041 agataaaatt tctcctttag tagggttttg agactcataa tcatctatat atgcttccag
    23101 tctatctata caaatatagt tattttttcc ctttatatat ccataactta cttttccatt
    23161 tagacccaaa gaatctaaca catttggaat atctttattt attaattgta tctgcaattc
    23221 ctttgtgtca gtagaaatta ataatctttt tttattaatt ctagcctcta atattgcagg
    23281 caataaatat cctacacttt tacctatacc agttggtgcc tctatacatg caattttttc
    23341 ttcatcttcg ctatttctaa acaactctct tattgtttta gtaagctcat attgaccagg
    23401 tctatattca tatgtaaatc cttctttatt ctcccaaata gttttgtatt ttaataattc
    23461 ttcataattt ttttcttctt catttaaaat ttttaaggtt tctctttcct tcttcttttc
    23521 ttcttttgag tcaaatatat tatattcttt ttttatattt atattatttg ataaatcata
    23581 atttgcctct tctaaaaatt tgctccattc ccaggttgga agattaaatt ttttaagata
    23641 tgaatttatt ttaaaactca atggctccaa agtagttttt tcatagttat taaattttac
    23701 taaaaggcaa tttacaatat ttattatatc aatagcatct gataaagctc tattttccac
    23761 tttagaatta caatttgtta aagtattttt taagtattct aagctataat ctttatgata
    23821 tggttctaaa ataactgcta attcaattaa atcaataaat ttgtttttta actttggcat
    23881 atgatactca aaaaactttt ttttcagact taaatcatga catataatga aactatcttc
    23941 tacaaaattt acaagtctat cttccaccag ttctatttca ttagctattt ctaaatcatt
    24001 tttatctaaa tttttacata ctgagaatac ttctacaggt acctcaaatt tgttttttat
    24061 taatgattca taggtatata ttttccagtc tttaaccttg acagctccaa cttctaaaat
    24121 ttctgaattt aaacaatcta atccactaac ttctatatct aaaaaaacaa catcattcaa
    24181 tacacttatt atattatcca tttttatatc ccttcaagtt aaaccagcct aacttattta
    24241 caatcatttc tctttatatt ttatcattta tatacacttt atttaagata attaatatca
    24301 actttatatc ttacagtaaa cttctttcat ttattatttg tatatgtgaa tatttttaac
    24361 tataaatatt caaactatat tataagaaat atactttaaa ttttaaataa gaggtgaaat
    24421 tgtgaaaaaa gatagcctaa aaaaatacat tatgatagga gcatttgctc taatactttt
    24481 tggaattgct agtataaatt ttaaatcatt agataaaaca aaaactcaaa tttctagtcc
    24541 aacactcgac acacatgaat atgattggta ttttaaccct agggaagatg gtaaacagcc
    24601 atcacctata aaagaagctg atttttttaa aaaatacgga gcttattatg taggaaatcc
    24661 caatgaaaaa gttatatatc tatcatttga tgctggatat gaaagtggaa acactccaaa
    24721 attattagat acgcttaaaa aacacaatgc taaagctcaa tttttcgtag ttgaaagtta
    24781 tataaaaagc aatccagagt taataaagcg tatggaaaaa gaaggacatt tagtttgtaa
    24841 tcattcaaaa agccatccat caatggcagg tattacagat tttgaaaaat tcaaagaaga
    24901 aattacaagt gtagaaaaag catacaaaga tgttactgga aaagaaatgc ctaagtattt
    24961 tagacctcca atgggtaaat ttagcgaaca atctttaaaa tatacacaag atttaggata
    25021 taaatcaata ttttggagtt ttgcttatgt agattggtat gaaaagaaac aacctactca
    25081 tgaatttgcc aaaaacaaaa tttattcaag aactcatcct ggtgcaatag ttttactgca
    25141 tccaaattca tcaacaaata ctgagatatt agatgaggtt cttacgcatt gggaaaaaga
    25201 aggttataaa cttaaaacac ttgattattt aaataataaa aaatagtatc ataaatttaa
    25261 aaaaactcta tcatgaatta tagtaaaaat atagttattt ataaatactt taattctcga
    25321 tagagttttt tattttttat agctatttaa aaagaattgt ccattcattt atctctttgt
    25381 atcctatatt atgatatatt ttacctgcta catcattatt ataaaaaaga catggaactt
    25441 ttccttctaa caacaattct ttactcaaat tgtaaaccat ataacttgca agtccttttc
    25501 ccctatatct tttatctgta cttacagaga ccaccattgc caaaaaacta gtttccatac
    25561 cagttgacac agtgctaatc atagtatcat catttctaat aaaatatgct cttacatttc
    25621 cctcttttaa atgttctttc ctttcttcta tgtaattttt acttacttta tagtctgacc
    25681 tatttaaaag ttttcctatt tcatcaatat ctttttcctg agcattttct attttatact
    25741 ccttcatgtt ggaaaaatct atttctttta tttcttttaa tacacaaaat ttattatcta
    25801 gtttttcata aaaatctttg tatttactta ttagtctatc tattacacat ttttttccac
    25861 ttatgctttc tacatctaaa tcttttatat gattcttcat ttctcctata ttaaaattat
    25921 ttttaaaact ataaattata agtgttgact tattttttaa tattatagag gttatatttc
    25981 catcttctac ctgagaccat atatcttgat attgagagct aaatccataa ttctcaacgt
    26041 atccaattat gaataaattt agactttctt catgtgatac atattgccaa aattcttttt
    26101 ctaaagtatt atctattttt acaatcaagc agatatcctc cttctatgtt aataatgata
    26161 taccttataa tttatagtat catttaaaat cattatattc taccaaatat tctaaatttt
    26221 taaatatcta tgcaatatgt tcgtatttat ttttatattc tgttactgct tcactccaag
    26281 cttcttctac tgaaatcccc ctcttatttg caattttttg tgctaattct attattttca
    26341 tagcttttct tgatatattc atatatattc ctcctctaaa ttatcataag ttattttaaa
    26401 gttaaatttc tatagtcatg ttatatattt atttacttat atcttactaa ttatttatgt
    26461 actaaatatg aactacaata gaagaaattt agaacttaat aaaacataaa atttatatac
    26521 agttcatata tatttgtcat atcacaagac aagtgatata atttattatc attttactgg
    26581 gggtgatatg ctatgaaaat aagagctgaa tatacatgtc ctcttgaaat tgtacatgac
    26641 ataatcaaag gaaaatggaa aactatcctt atatttgaat taagacaatc taataaaaca
    26701 ttttctgaat tgcaacataa tatttatgga ataaatcaaa aaatgttgat tcaacaatta
    26761 aaagagttac gtgattttgg gattattgat aaaataagtt ctgctggata tccattacat
    26821 gttgaatatt ttcttactaa tcgtggtaaa aaaatgttaa aagcagtgga aataatgcaa
    26881 gaaataggaa tcgaatatat gcttgaacat ggtcaacaag aaattctaga taataaagga
    26941 atttgctaca ataagtaatc ctatacatac caaaaagtaa gtaatttaca ttttttacaa
    27001 tagtattata ataaaaataa ataggaggta taatcatgtc tatagcttta atattgattg
    27061 atattcaaaa tgattatttt aaaaatggaa aatgtgaatt atttcaatca gaagaaactg
    27121 ctgaaaatgc taaaaaaata ctactgtttt ttagaaaaaa caatttacca gttattcata
    27181 tacagcatat atcaacagat aaaacagctt ctttttttct tcctaatact catggttcag
    27241 aaataaataa aattgttttt ccactagata atgaagaaat tataattaaa cacacaccag
    27301 atagtttctt tgaaacagat ttgcaaagta tccttgagaa aaaaatatta ctaatctagt
    27361 aatctgtgga atgatgagcc atatgtgtat tgatacaagt gtaagaacag caaagaaact
    27421 tcgatacgac ataactctaa taagcgatgc atgtacaacg aagaatttat cttggaataa
    27481 tatagaaatt aatgcaaata ctgtacatca agtattcatg gcatcactac agaacagttt
    27541 tgcagatgta aaaaacacag ataaattttt atctaactat cacaaataaa aacatacact
    27601 atgttaaaag agcaagtttg atttatttaa gcttgctctt taatcattta aaattagatg
    27661 ctttctatca tttttatata cataatatac gtttatacat tttatattca tatataacta
    27721 ttatataaat tttaaacttt actatattca cataaaaaat gtagtattat agtatatata
    27781 atattatata gaagggggtg ctagataaat gaaccatcat atattgattc aatttgaata
    27841 tatagcaaga atcattactg ctggtttctg tggggcttta attggatatg agagaaagaa
    27901 tagactaaaa gaagctggaa tacgcactca ttttatagtg gcattagctt cagcacttat
    27961 aatcattgtt tccaagtatg gatttaatga tatagtgggt actccaggta ttggtctaga
    28021 cccagcccgc gttgcagcac aagttgtcag tggtataggt tttttaggtg ctggattaat
    28081 atttattaga aatcaatcag taagtggtct tactacagct gctggtatat gggcaacagc
    28141 cggtacaggt cttgccattg gctctggatt atacttagtt ggtattgttt caagtatcat
    28201 tattgttatt gtacagataa cacttcatag agataggaaa tggatgaaac tccctacagc
    28261 agagcaagtt tctcttaaaa tttctaacag cactgattcc atagaatata ttgaaggtaa
    28321 atttaaaaaa tctaatattg aaattataaa catgaatatt aaacgccttg atggggattg
    28381 gttagaagta gttatatata ctaagttacc taaaggatat aaaaaaacaa atttattaaa
    28441 attatttagc gataatcctc ttatagaaac tttagaaata taatataaaa attgaagctt
    28501 aaaaataata atatatttaa tcaaggtcaa tatataaata attaagggat gagtctatat
    28561 aaaattcatc ccttgttttt tgccattcat atatattttt tcaagatatt tttgtttatc
    28621 tattatctag ccaagtaacc cttgcgaact aaacttttta atacaaaaac ttcaatacaa
    28681 aactaaatat aatttttatt aaacattcta tacatatagt ttgtttcttg agtgaaaaat
    28741 agatttcata ttgttaagat attgttaaat atttcaattt tctgacaagt atgttgaaca
    28801 ataacttatc ctagtgtatt attttagttg tagaataaaa ctaaatcggc aaaactagag
    28861 aaatttagtg acgcaaagct atagggacta acgcttataa aataaactat gagttatgtc
    28921 agccagttgc caaaaagata ttgttctttt tgttttatga aagtttttta gggggatagt
    28981 aatatatatg tttagagatg aaatggataa atgtacacac atgttaactg cttatattag
    29041 tagtttatat gattattgtg attttataga tacacagcta gatgatttta tactagagta
    29101 cggagaaaat gtagtagaat cttgtttaca tcaagtgatg gtattggtaa gtaagtataa
    29161 ttaaatagaa attgttttct agagaaccct ttttagtttt atctacattt aaaaatgtta
    29221 ttatattaaa aaactaatct tccatatatg caaagtttct tccatataac aatttcttcc
    29281 catttatact ttttcacttg tatctttaat aaaattcaac ttcaaatatt aatatacttc
    29341 aaaaattatt ctatttctta tctttacacc aattcctatc agtattatcc tttatatagg
    29401 ttctaactat ttcgccacaa tctaaacata aatctgaata tatagtagat tgtactggaa
    29461 aagttttatt tataacttta tctaattttc catctcctct atattttaat ccaatagttt
    29521 cagcatatat gccaccagtt gttaaaattc ccttttcaat atttacactt ccacaattag
    29581 aacatttcac atttataacc ccttaaacaa ttattttttc atgctatctt ataatttaaa
    29641 atataatttg ctaccgattt tataacacag tggcaaatcg gtggcaaatc aacatttttt
    29701 cattctattt accatctaat ccattgaaat atagctattt agttatagga acttgaattt
    29761 ccgttacaaa ctcctctaca attccagtct catgtatatc tatcttgtag atttctatag
    29821 ggtcagcaat tatcttataa ttgttttctt ctataaattt aaacaccttc tccatataca
    29881 tcttattatt agaattactc ccagtataat ttaaagttac ataatatcct tcatcaaaaa
    29941 ctatattata tactttctca ttactttcca gaaaacaaaa tacagattta aactcattaa
    30001 atataccttt attaattgct tcagtagaaa aggcagaacc aatattgtta tttccaagta
    30061 tattaaatct atcttcatat tctttctgaa gtttttgaat taaaaaatcg aagtcttcat
    30121 ccctctttat atcagcattt aattctaaag cttttctttt ttctatatat acaacctcta
    30181 tctcatctat ttttgtattt tgaattacac tttcaattga actcaatctt tttataatat
    30241 tttccttatg gcttataaat tcagctattt ttttatcaat taaatcgagt tcttcattta
    30301 aaatattttt cgtagactct atacttctgt catctaggta ttcttttatt ttcttcattg
    30361 gaaagttcaa gcttctaaat tcttttatta aatttaattt ccatatatct gatatattat
    30421 acattctata tccatttata tctctaactg gtcttagaat gcctatttct tcataataca
    30481 ttaatgaatc tctacctatt ccatatattt tagatatctc tcctatttta tagtaatctt
    30541 tcatttatat aataaactcc tccgaaatct tatttttatt ttaaatcaac attattactc
    30601 tataatacta tagtattaga atat
//
LOCUS       DACXMZ010000020        29012 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.020, whole genome shotgun sequence.
ACCESSION   DACXMZ010000020 DACXMZ010000000
VERSION     DACXMZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.020"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            141..2783
                     /gene="adhE"
                     /locus_tag="JD016_03615"
                     /gene_synonym="adhC"
     CDS             141..2783
                     /gene="adhE"
                     /locus_tag="JD016_03615"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAU4998719.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLNAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3143..3484)
                     /locus_tag="JD016_03620"
     CDS             complement(3143..3484)
                     /locus_tag="JD016_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998720.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3511..4182)
                     /locus_tag="JD016_03625"
     CDS             complement(3511..4182)
                     /locus_tag="JD016_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4998721.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4194..6770)
                     /locus_tag="JD016_03630"
     CDS             complement(4194..6770)
                     /locus_tag="JD016_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAU4998722.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKMGQCEIFSNLNESKLSDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMVGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFMIIVLSMSIGGIIVIKDNYKYSFLDVQNKSVQEQTYM
                     NADFILSNVYLNETGNNTSFKDIKGFSDSQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NKLDYYETINSKPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYTFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLIYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIIAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISEREFKNMILYEGILYGVLSSIITIVSGLI
                     IQLKMYYMQGFISHGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            6978..7913
                     /locus_tag="JD016_03635"
     CDS             6978..7913
                     /locus_tag="JD016_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAU4998723.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            7932..8669
                     /locus_tag="JD016_03640"
     CDS             7932..8669
                     /locus_tag="JD016_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4998724.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(8854..13911)
                     /locus_tag="JD016_03645"
     CDS             complement(8854..13911)
                     /locus_tag="JD016_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAU4998725.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIIQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(13936..14370)
                     /locus_tag="JD016_03650"
     CDS             complement(13936..14370)
                     /locus_tag="JD016_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998726.1"
                     /translation="MRGDFMQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIV
                     SKNVLEYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAF
                     EDGIYKVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(14588..14839)
                     /locus_tag="JD016_03655"
     CDS             complement(14588..14839)
                     /locus_tag="JD016_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998727.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            14928..15659
                     /locus_tag="JD016_03660"
     CDS             14928..15659
                     /locus_tag="JD016_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAU4998728.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            15718..16629
                     /locus_tag="JD016_03665"
     CDS             15718..16629
                     /locus_tag="JD016_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAU4998729.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFENNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            16680..17492
                     /locus_tag="JD016_03670"
     CDS             16680..17492
                     /locus_tag="JD016_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAU4998730.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            17601..18293
                     /locus_tag="JD016_03675"
     CDS             17601..18293
                     /locus_tag="JD016_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAU4998731.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            18359..19321
                     /locus_tag="JD016_03680"
     CDS             18359..19321
                     /locus_tag="JD016_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998732.1"
                     /translation="MRKETRLMRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVV
                     LATTIMISNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEE
                     ISNNKNLNNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYT
                     SKEKNSEDIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKD
                     VTNARNKCKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLIN
                     TKSPKYIGKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            19358..20515
                     /locus_tag="JD016_03685"
     CDS             19358..20515
                     /locus_tag="JD016_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAU4998733.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGKKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            20667..21227
                     /locus_tag="JD016_03690"
     CDS             20667..21227
                     /locus_tag="JD016_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAU4998734.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            21255..21746
                     /locus_tag="JD016_03695"
     CDS             21255..21746
                     /locus_tag="JD016_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998735.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            21975..22526
                     /locus_tag="JD016_03700"
     CDS             21975..22526
                     /locus_tag="JD016_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU4998736.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            22612..23544
                     /locus_tag="JD016_03705"
     CDS             22612..23544
                     /locus_tag="JD016_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998737.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAVVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFGKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            23599..24462
                     /locus_tag="JD016_03710"
     CDS             23599..24462
                     /locus_tag="JD016_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAU4998738.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            24709..26571
                     /locus_tag="JD016_03715"
     CDS             24709..26571
                     /locus_tag="JD016_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit EIIBCA"
                     /protein_id="HAU4998739.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGKRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            26574..28040
                     /locus_tag="JD016_03720"
     CDS             26574..28040
                     /locus_tag="JD016_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAU4998740.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDVDV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASAIATKICHEIISNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIESNSIDFK"
     gene            28064..28900
                     /locus_tag="JD016_03725"
     CDS             28064..28900
                     /locus_tag="JD016_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU4998741.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFIFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT        10710 a         3484 c         4651 g        10167 t
ORIGIN      
        1 taagagagta aaataaataa aatctaaggt atagagatga aatctataaa attattttaa
       61 gtataagtta aaaatataga tgatgcattt aagatttata aaaattagag atgcataatt
      121 attttaggga ggtttttagt atggagaaaa aagaaaaggt agtagaaaaa tcaaatgttg
      181 aagtatgtag tcctgaaatt gtcaacagtg ttgaaacatt aagaatgaga ttagaagaaa
      241 ttagattggc acaaaaagaa tttgcaacat ttacgcaaga acaggtagat aagattttct
      301 tagctgcttc aactgcagct aatcaacaaa gaatacctct agcaaaaatg gcagtggaag
      361 agactggtat gggtattgtt gaagataagg ttataaaaaa ccattttgcc tcagaatata
      421 tctataatgc ttataaagat actaaaactt gtggtgtcat agaaaaagat gaagcatttg
      481 gttttacaag aattgctgag ccagtaggtg ttttatcagc agttatacca acaactaatc
      541 ctacatcaac tgcaatattc aaatccctaa tagcactaaa aactagaaat ggtataattt
      601 tctctcctca tccaagagct aaaaactgta ctatagaagc agctagagtt gtacatgatg
      661 cagcagttaa ggcaggagca ccaaaaggac ttataggttg ggtagatgtt ccttcgattg
      721 aattaactaa tgttgtcatg gcagaggcag accttatatt agctactggt ggacctggaa
      781 tggttaaatc tgcatactct tcaggtaaac cagctgttgg agttgggcct ggaaatgtac
      841 ctgcaataat agatgagagt gcagatataa aaatggctgt aagttcaatc ttagtatcaa
      901 aatcattcga taatggtatg atatgtgctt ccgagcaagc tgtaatagta ccagaaaaaa
      961 tatatgaaga agttaaaaaa gaatttaaat atcgtggagc acatttccta aataaagaag
     1021 aaactgaaaa agtaggaaaa gtagtaataa ttgatggttc attaaatgcc agaatagttg
     1081 gacaaccagc acatgtaata gccaaaatgg cggatgtaga agttccaaaa acagctagaa
     1141 taataattgg tgaagtagaa tctgttgaat taaatgaacc ttttgcacat gaaaaacttt
     1201 ctcctgtact tgcaatgtac aagtctaaaa gttttgaaga tgcagttgct aaagcagaaa
     1261 aattagttgc agatggaggc tatggtcata catcttcatt atatgcagac tctataaatc
     1321 atccagagag agtagaaaag tttgtaaatg ctatgaagac ttgtagagta ttagttaata
     1381 ctccatcatc acaaggtggt ataggtgatt tatataactt taaattggca ccgtctctta
     1441 ctcttggttg tggttcttgg ggtggaaact cagtatctga aaacgttgga gtaaaacatt
     1501 tgcttaatat caagacagta gctgaaagga gagagaatat gctttggttc agagcaccag
     1561 aaaaggttta tttcaaaaaa ggatgtttag gtgtagcagc aagagaattt aaggatgtta
     1621 tggataaaaa gaaagcattt atagtaacag attctttcct atataataat ggatatacta
     1681 agaaattaac tgatttatta gatgagatgg gaattaagca tactacattc tttgatgtag
     1741 ctccagaccc aacattagct tgtgcaagag aaggagctaa agctatggct gatttccaac
     1801 cagatttaat aatagctgta ggtggaggaa gtgcaatgga tgctggtaag ataatgtggg
     1861 taatgtatga gcatccagaa gtagatttcc aagacttagc tatgagattt atggatataa
     1921 gaaaaagagt atatgtattt cctaagatgg gtgaaaaagc gtattttgct gctatcccaa
     1981 cttcagcagg aactggttca gaagtaactc catttgcagt tataactgac caagattcag
     2041 gtgtaaagta cccattagca gattatgaat taatgccaaa tatggctata attgatgctg
     2101 atatgatgat ggagatgcca ccaagattaa cagcagcatc aggtgttgat gctttaacac
     2161 atgcattaga agcatatgta tctatgttaa gaactgaacc agctgatggt ctagcactac
     2221 aagcaggtaa aataatattt gaatatttac ctcgtgcata taaaaatgga aagaatgata
     2281 aagaagcaag agaaaaaatg gcaatggcat ctactatggc aggaatgtct tttgcaaatg
     2341 ccttcttagg aatatgtcac tctttagcac ataaattagg tgcattccac catgttcaac
     2401 acggtgttgc aaatgcatta ttaataaatg aagtaatcaa gttcaattgt gcagaagctc
     2461 caaataagat gggtgcattc tcacaataca gatatcctga ttgcatacaa agatatgctg
     2521 aatttgcttc ttttgcaggt attaaaggaa gtacagacca agaaaaagta gataatctta
     2581 taaaagcaat agatgaacta aaggcaaaag taggtcttcc taagactata aaagaggctg
     2641 gagtagaaga atctaaattc ttagaaagat tgaatgctat ggttgaacaa gcttttgatg
     2701 accaatgtac aggtgctaat cctagatacc cattgatgag tgaattaaaa gagatatatc
     2761 taaaagttta ttatggtaaa taatatctat tgtgcaacat aggttctatt gggtttgtat
     2821 ataaattatt agatttgaat ataaatagag ttttattaga gacacgtaga agtaactata
     2881 aataatagat tataacttaa attctaagtg gaaatgataa aaagtcagta atttaatggt
     2941 aatttcgaat atgtgtattt aatcaattaa ctttaaaatt agtcggaaaa tttttttatt
     3001 acatataatt ttaaagcata atatataaaa aactttatga aaaaattata aaaatattca
     3061 gaaaattcta ttgactaatt tatttatcat gatataataa gtttagaaaa gaaaaaaatt
     3121 ggggcctatt aaggccccct tactattttt tgatagattt atttaaaatt actttctcat
     3181 tttgattttt tatatttatt atagagtcag tataatccac ttcaaagttg gcaaaagcag
     3241 agaataaatc tatatctctt aacaaatgaa ttgtcataca ttgattgttc ttatttagcc
     3301 ttagaatcaa tgctcttaca aatggaaatt gtattctaga attaaataag ttagaaccat
     3361 cagtatctat atctaagcta cgataattaa tatctataaa tatatctaca tcatcatttt
     3421 cttctcttat tattctgtag tcaaaatcca agtttaaatt taaattacct aaaatcaata
     3481 acatttaaat cctcttttct ataaatagtt ttagagtgtt aatttaagct ctccatctat
     3541 tatagatata attctatctg cttttctagc aatattttca ttatgtgtta ttattataag
     3601 tgtttggtta tattttttta tagaactttt tagtaaatcc atgacttctt ctgtagtttt
     3661 actgtccaaa tttcctgttg gttcatctgc taggattata cttggttttg cagataaagc
     3721 acgtgcaata gcaactcttt gctgttgacc accagataat tggttgggaa gatgattttt
     3781 cctttcatta agacccaata aatttataag gtcatcaata tattctttat ctattttttt
     3841 attgtctaac tttagtggta attctatatt ttcctttaca tttaatacag gaattaagtt
     3901 atactgttga aagacaaatc ctaccttttg tcttctatat cttgctaatt ctttatctga
     3961 aagacaacat atatctttac catctacctt tatagtacca ttacttggtc tttcaagacc
     4021 acctactaaa tgtagaaatg tacttttccc acttccactt ggcccaatta ttgcaacaaa
     4081 ttcaccttta tttattttta aatttgcatt ctttagtgca tgtacagatg tttccttttt
     4141 cccataaatc ttacctaaat taatagtttc caatataggc ataattatcc ctcctattct
     4201 gtaatgttta tagcttcaac tatactaatt ttatttatct ttcttgatgg aatatatgta
     4261 gctagaatac ctacaattat atttgctaca acaactagta tatatatttt gtaatctatt
     4321 gaaaatccca atccatgtga tataaagccc tgcatataat acattttaag ttgtattata
     4381 agtcctgaca cgatagtaat tatacttgag agtacaccat ataatattcc ttcataaagt
     4441 atcatatttt taaattctct ctcagagata ccaatagctc ttaacattcc aaattcacta
     4501 gttcttgaag ttaagttata acttatgttg tttattatgt taagtacact gattatgaac
     4561 attattgcta ttatgccata actataaatc atagttctag atgtcatctt atcagaattt
     4621 tctttttcta ccatcatatc tactgttgta gttccaggaa cttcacttcc aattttacct
     4681 aaaaggttat ttattttttt cttgtttgca ttttcattaa ggtctgcata aattaagtta
     4741 tatttatcta caccagttaa tttttttagg tattcgctac tcgttattac atctacacca
     4801 tcatctccag agtacatacc attatctgca aaagtataac ttataatagc tccaacttta
     4861 aattcatatt ctaagtattc ataattatct tttagacttt cataaagccc tgtatccatc
     4921 ccaccctttg gatatttgac ttttatagta tcacctactt ttatatctac agctggtgtt
     4981 ccttcaccag gagtaaaagt ctttgtgtat ttctgtgttt tagaaatttc tggtacatat
     5041 acaatagcta aattttcttt tttcattttt ttcaagttta tatttccact tactaagtat
     5101 ttttctaatg actttatcat ctcatcatta taacctttta gcttttgctt tagtgtatat
     5161 ttgccagtag tcttattttt tatgaacaga ggatttagtt tataataagg ttttgaatta
     5221 atagtttcgt agtaatctaa tttatttatt ttatttacct ctattcttgt gtttaagata
     5281 ctagcagttt tgactttgtc aattccatct atatttttta ttttatctat ctgactatca
     5341 ctaaaccctt ttatatcttt aaaagaagta ttgttaccag tttcattgag ataaacattt
     5401 gatagaataa aatctgcatt catatatgtt tgttcttgca cggatttgtt ttgcacatct
     5461 aaaaaagaat atttataatt gtcttttatg acgattatac ctcctatact catagataat
     5521 acaattatca tgaacccctt tttatttcta aagatgtttt tcgcagatat agattttgtt
     5581 actgacatat ttaaagttaa ttttgatatt aaataattaa ctttggttac atttttatca
     5641 gctccaaaag tttttctgat agcatcaata ggagaaattc ttcttatttt taagtatgta
     5701 aagaaactta taattagtat tattagtatt gtacatgcaa ttgataagag aattatttta
     5761 tctggaataa caaaaggtgt aacatttata tttccttcaa attgaatatt tcctacagac
     5821 ctattaaata tttgtgcacc taccatacct atacatatac ctataggcat tgctattaga
     5881 gcaagtatga aaagttcata aagcataagt ttgaaaatcc taaagttggt ggaaccaata
     5941 gctcttaaga cgccatattc ctgtactctt tggtatattg atataacata gatactatat
     6001 attacaatac cagcaaatag actcatagca attgcaacgt atttacttga attatctaaa
     6061 gtaccgtttt tcatagtaga ttctataagc attgagtttc ttcctatact tttctcatct
     6121 aacatagcat tttttattat attgtctatg tttgtattta tatcactgcc ttcattaaat
     6181 tcaatataag ttgtaaaatc acttagtttt gattcattga gattagaaaa aatttcacat
     6241 tgtcccattt ttttctctat aaatctatct tcaaggattc caacaacttt aaaagtttct
     6301 ggattttctt tttgatataa tttaaaagta aggttttgat ttatctctgg ttttaagccc
     6361 atactattta aaatccattt ctcgactacc acctcattgc tagcttttgg aaatcgacct
     6421 tttataagtt tagaatgact agtcaaataa tttttatttg catatagtat gtttataggt
     6481 aatttttcat taacatctgt agacgcatag taagatgtta tacccagatt tttaatatct
     6541 ttgctagatt ttatggactt taattgattt ttctttatat ttttaaagta tacatggtca
     6601 gttccaagct ctcttttcaa tctatctaca tctgcttgct gagcacttct tgagagggta
     6661 ccaataccaa caacgagtga tgtagcaaga attatactca caacaattgc taaagaccta
     6721 gctttataac tttttatata ccttagtact attttcaaat tatctttcat atttaccact
     6781 tcctttctta actaaatcaa ttatatattt atctaaaata tatagcaaag taagtatttt
     6841 caagttttaa gtagttaatt gtacaaattc atctgtattt ttacaagttt aattaatcta
     6901 tatgtaaatt ttaacttttt acataatttt tttaaaatga tataatccct atataaattg
     6961 tagaggggag gggaatcatg gataacaatt ttgtatttcc tatagtaata ctaactttgt
     7021 ttatatttag caatcttata atgaataagt ataaatttta caaagaatta gacatattaa
     7081 aaagaggcat attaacattt tttatatcgt gttttgcatt gattttattt atggagggca
     7141 ctaaatatat acaattaagg ttaggatata gttttttact tgaaggagta ttaaatatta
     7201 tacttttttt ggttttaatt attttaagtt tttatacagg ctttaaagtt ataagattga
     7261 taaataataa taatgaacag gaatataaac tacaaaaact agatagtaaa ataaatcata
     7321 aggataatat aatcatcaaa ttaagaagtc aaaaacatga ctatttgaaa catttgcaag
     7381 tagtgtattc tctattaaat actgggctag atgaagatgc caaagaatat ataatgaata
     7441 tatcaaatag ctttaaatca aaaaattcaa ggtatggaaa agtatcttat ttagatgcta
     7501 tagcttcatt aaaatacgag gaatgtcttg aaaaggatat atcttttgat gtctacattc
     7561 aagattttat ggataattta gagattgaac ctaatgatat gagttcagta ctattaaaca
     7621 taatagataa tgcaatagag tctttgtcta ctataaaaaa agaaaagaag tatataagat
     7681 tacatgcata tgaagatgaa tttcagtata ttatagctat aaaaaataat ggtcctaaaa
     7741 tacataatgt tgaaagtata ttttggaatg gatttagtac taaagaagga caagataggg
     7801 gatatgggct acatttagtt aaaaatatat tagataagta tgattatagt atacttgtaa
     7861 atagtgatga ttttttaact gaatttgtaa tcttaatccc aaaatattat tagttaggag
     7921 gagaaaagtc tatgataaaa gttttgattt gtgatgatga taagattgta agagatgata
     7981 tagcaaatat agctaaagag tctggatatg tagattatgt taaaaaagtt aaggatggta
     8041 tagaggcaat tgaatttata aaaaatgaga aaatagattt attgattatt gacattgata
     8101 tgccatataa aaatgggatt gattctgctg aagaaattct ttctatagat aaggatatac
     8161 atatagtatt tgtgacgggt ttcgctgatt atagtcttga aagctttaaa gtgcatccta
     8221 tagattttgt agttaaacca tttaaaaaag aaaaaatact tgattcaatt aacatagcaa
     8281 tagaccatat taactcccat aagattgcta ataataattt tatagaagac accctttttg
     8341 tatataaaat aaggaagcag atacatatga ttaattttga tgatatagtt atgtttgaga
     8401 aaaattcaag agcaattaat ctacacacta aagatgaaga tataataaag ttttatgaga
     8461 actttgatga tttaaaaaaa cgcctacctc ctaatttctt tatgacccat agacaataca
     8521 tagtaaatct aagatttata cataaggtta tacctataaa taaatcttca ttagagataa
     8581 ggtttataaa ttttcaagaa aatgccatat taaacaaatc tctagaaaaa gattttttat
     8641 atagatttta tagggttaaa agattctagt gaagatagaa ggaaaatact ttttgtgtga
     8701 cataataagt accgtaaaga taatgtataa gagaagttta tataaaaatt atttgtaatt
     8761 atgatttaat ctatataaat tacagtatag tatatattgg actttctaat ttttttaaga
     8821 aagtccaata tattttagag atagttttta tacttataaa ttacttaaga tactagggtc
     8881 tttaatctta ctatcttcag caagtaatag tatcttagac ataatctcag cagttttagg
     8941 gtcttcatca gcgaatggaa ggaatatctt acctctactt tgagaatgta taggaagtat
     9001 attcaaagca cccatccctt ttttatgtac aataccagaa cccatatgca cagaatactc
     9061 acctaaatta cctgctataa gagcatgaga gccttcaaaa ttgacattgt taatttttaa
     9121 taataataaa agttctttag caattgctat acgcatctct acagtagaat ggctagtttc
     9181 agggtcaaca ccaccagcat gagcgacact tacaacaagg tctatatcac gcataacttc
     9241 tgagaataga attggaggta tagaagctaa ttctaaactt ttataagttt ttctatcaaa
     9301 aaattctact gtttcaatag taggaggctc tatatctgct ggtgaaaacc aatctgcaag
     9361 tgcaaacatc tttgccacta tattttcttt ataaaatact ttctgtaatc cctcttcata
     9421 atcgacagtc caaagctgat ttctaagaag tgctacagtt ttctttggct gaatttggtg
     9481 accagcatat ctgcgagata tagttttttc agataattca tcactattta ttctataata
     9541 ctcacggaat acttgcttaa atggttgaat aatttgctta ctaaataaat catgttggaa
     9601 ctcactccac ttaccagatt caaacaaatg aactggatgt gcaagtttta atttattttt
     9661 tgccgtcaac ttataagttt caccttctat attgcaaata aagaacttag acttatcatt
     9721 ataaacatat ccaatattat catcacatac ccatactaga tttttaatca aagggcagat
     9781 aactggattt ttatagatat tggcaatttc tttaactgta aatactattt catttttcat
     9841 agcttgttct aaactctgtt ttgcacgaga atactgactc tttaaatctt tttggacaga
     9901 tttaagttca agtacatatg gatgtttagc aatagctttt ggtatactct ttaaagactt
     9961 gttgcctttt tgaactttag tagatgcttt tccgttttca tcaacatcta tccaaattga
    10021 agtaccatca atatcttttg gtttaaatag atgagcaact tcatttagtt tttcagtctc
    10081 aagagcccac atcatacgat ttacatctcc aaatccagca cttctagcta gattttctag
    10141 cgctatagta caagcttttg attcactttc acgacgttgt gcaccaaatt gcttgctttc
    10201 tttagcaaac ttttgaataa attcataacg gaaagttgct tcttttaggt ctcctttttt
    10261 cataggaagt aaagagaaac ttctcaattt atcttgattt ctgtttgttt caatttcttt
    10321 catcaacatg tctttatcaa gcttaccaag tgtagcatca gcatagagtt gtgcacgtct
    10381 atgagagtta ccaccactag ttatatattt agctgacttg taaagtatat tgaatctttc
    10441 ttccccaata gttttatatg aatctaaaaa ccaatctaca tcaaatgcac catcaatgaa
    10501 actacgtggt gaaatagggg agaatagagc aacctctgat tctttttgtg cagagaagcc
    10561 ttcatttata tgtgcatgga agtaccaaat tgtacttttc agacccttcc agtttaaata
    10621 tttttcaatt atgtttaccc attgaggggc atacatagca gattctatca atcttaactc
    10681 agtaatgtcg gtatttttaa gtaaactttt taattttttt gagtcatctt ctttagtagg
    10741 atagcaattt ttaagtagag aacttaaaac atcttttttt gaataattgc taccatatga
    10801 atatcctctc tggaaagttt ctttaccaag agcgaccaaa atatttacaa aataatttat
    10861 accttcaaag tattgtatat tattagctag attagacact tctgttggca tatcacctct
    10921 acgtgattca atttctataa ttcggtttgt aacagttttt aaattatctt taagccaagg
    10981 gtacttttct aatgtgtcgc cagcccatct aggatttgtc aatgttctta ttatatttga
    11041 tgcgttgtat ccacttatca tttctctatt aacttgctct tcagtcatta atcctaattc
    11101 atgtgcacga gcaaacacat ttatgctagt atagaaagga aacttatatt ctagcaactg
    11161 ataatttgaa aacgtagtgt agaaaaactt agtaaaagaa atatcatcat aaataaacga
    11221 atgtgcttta tcaagccaat agtttaaaat aggggcattt aaagcagggc agtattcatt
    11281 ttgtctgtaa tagtttttag taaattcaac tctttgtttt ttacattgtt ctatagtcaa
    11341 taagtttcta agtttatagt atatttctaa acaatagtta aatatttctt tatggtcaaa
    11401 ctcatttagt aaaactctta aaagttttct tgtaagtcca ttaaatttaa gtttttcaag
    11461 ttttgtatat attttatttt gttttttaga gtcaaggaca tttgaatata tagatgaaaa
    11521 ccaacttttt tctgtttcat tatatccaca tgtacttata ctataaaata agatatcaag
    11581 tattaatttg taatctaagt caagtttttt actagtttct ctccaaagct ctgctaatgg
    11641 ataattatca agagtatcat tagaaacttt atatttaata ggagtcaagt tataagtaga
    11701 ataacctaaa agtactttgt ctacattacc tgaccatctt tcaacttcat attcataatc
    11761 tgcattttca gtaataagct catcaaattt ttcataaact tttattattt tatttatatc
    11821 cgtatcgaga attttttttg cttctttttt tgttgctaca gaaacagaat cctcacgagt
    11881 agataaactt atatttgaga taattttatt aagctttgat gagctattag aatcatcttt
    11941 aaaatcaaat tgttctggaa tttgaacttt gttttcaggg ttatatattc caaaaccatt
    12001 ttctttgcag taatcatttt ttgctttacc taacaaatta tcaattagta tttgaacttg
    12061 agatgtattc tttttagatt tatcaatatc ttctataagt aaaacaagct tattatatag
    12121 ttcttgtcta gcagtattgt cttttaataa gtttacaatt tcaagacctc caaggcattt
    12181 ttgagaattt gaagatttta tcagtgaaga agcaatattt tccaagtttg aatcatcttg
    12241 ttcaagtaaa atttttatta ctgtttgtct aatagagctt gtctttaaag acaatagatt
    12301 ggttatctta tctaattcta catctgttag ctcaagcttt ctaagtagac tcaataccgt
    12361 ttctctattg gaaacactct tatcacttaa acattccaat aaaaattcac gttgtctatc
    12421 tgtctttggt tcatttacaa aattgactat aagtgaagct cttaaatctg aactcataag
    12481 atgacaattt tccataagca catctataaa ttgagcattc atatcgtatg ctgttaaagt
    12541 catcatacat ttaagtattt catgagaaga aatttcaatc caagtccatg gaaatacact
    12601 tcctgaaaat gattttgtct ttgaatttaa aatttctaaa gtatgtttta aactttcaaa
    12661 ttgttctttt ctagcatcaa agtctttgtc aataggagtg tttgacaagt caaaattagt
    12721 ttctctgttt tgccaactat agtagtagcc aacagaacct tctacataga aatttgtaat
    12781 tacccatgct agtatctcta aatcatcacc cttaatgttt ttagatgcta tagaatgttt
    12841 aaaatatgta ctttctgtat gcgataaaaa gtaaagagct acaagttttt tatatttttc
    12901 ctgacaagaa agtaagtttt caataagaga gtaagagtca tatatgttgt aagttgcagt
    12961 agcccacaag gcaaaataaa tttcaagatt atcattgcta ttaattgatt ctatacggaa
    13021 cttttcatca gtcaatgatt tatatgaaag ttctaaacat ttacgtatta ttccaggttt
    13081 ttggtctgaa aaaccaagtc ctgtccatgt atccaaggct cttataacag aactaaatcg
    13141 ctctaaattg ttgtccaaaa tagtttttaa catgtatatt gatgcagaaa ttgtaccctc
    13201 atctaaactc tctacaattt gctgtctaag tccttcttgt tgcttggctg ccaaaagaag
    13261 tttagaaagc atttcatagc atttatcact atgactcttc atgataccaa ttatcatttg
    13321 tcttgtaagt agagcagttt gattttctcc aaatattact tcttctaagg cacttattac
    13381 tttttgattt cctttgtcta tttctagtgc tattttatca ctcattatag agtcatagta
    13441 caagacctta tttggaagag taagataatc tataactgaa aaatcataat aaattaaatc
    13501 aacatagttt gaaataaacc ttataatacc tctaaaataa tccacaagtt tatttgagcg
    13561 atacccttta cgataatatc ctcctgaata tgtataatcc atctttcttt taattaattg
    13621 gcaaagttct tctacttgtt cagaagtaaa tattaattct aatgcatttt ttaaaggtcc
    13681 agaaaggagc tcgtccatat ttttaatttc gtagtagtgg caattgtagt atttctttat
    13741 catatttaag aatttgtctt caggttctct ataatagtat gaactatttt ctatgcagtc
    13801 ccaaagagac tttgctaagt cgtagtactg ttttgaagaa gatttagtaa aattattttt
    13861 aaaattttca attattttag gatttttttc tactctagta cgttctaaca tttatgtacc
    13921 tccaaattat ttattttaaa acattcttcc agcaagcaag gtaagtctta taaaagtaaa
    13981 tatatctcca cttttcagat ttatagagga atcgagtttt tcaataacat cttcatttat
    14041 aagcactttg tagattccat cttcaaatgc ctgtaacgct acctctattg ccttttctac
    14101 agattgttta ttctcattgt aaatagagcc aaatccaact tttccaatat cagcttgaga
    14161 ttcaatttct tcattagtta agaagtttat tattgatttt tctgggattt tctcattata
    14221 ttcaagaaca tttttagata caatttcaga gatagcttct tttaggcaat tgatgttgtc
    14281 agatacatca aaaggggtaa gttctagcat aggtttgcgt ttgccaacag ccttaactct
    14341 aaaataaatt tgcataaaat cacctcgcaa aatattaatt atgtatttag aaatttaaaa
    14401 tttatctatt tgttatatta tatcattttg tgaattatta atgtgaatta tggaattatt
    14461 ttacaaaaaa tagaaaaaaa gctatcttaa agtctaaaaa agcaataaaa gataactttt
    14521 aactataatt atatagaatt ataactgtac atttattaaa ttttaatttt agaattattt
    14581 ttgataacta tcttttttta tttataaatt ttgctcctaa atattttttg atatcttctt
    14641 caggagaaaa gtcatcaata agtatttctt tcccagtgtc ttcaaatact acaagaactt
    14701 cacagtcacc ttgtaagaaa aattttctaa ctgtatcttc tttaacttct ctaactttta
    14761 ttacattctt tttttctttt tcaaagtctt ttgtgtaata acttcccatc atattaatta
    14821 tttttgtata tctattcata aaatacctcc atatgcgatt aaatttaaaa aatatgttat
    14881 aataattgtt tgtattaact taataattat aacaaggagt aataataatg actagtataa
    14941 aaaataaaga tattttaaat tgtatagatt attccattaa aaataagctc tttgacaaac
    15001 taaatgatgt atataagtcc ttgccagtag gaaattgttc tggatgtgga aattgttgta
    15061 tggagtcagt aggtataaat ttaattgagt ttttgaatat attttgttat cttgaagata
    15121 gagttgattt aaaaaaaagc tgtataagta aaatattaga ttattatttt gaggagtata
    15181 caagaaagaa cccatgtcca tttaaagata ctaataatag atgtttaata tatgaagtta
    15241 gaccattaaa ttgtaggatg tttggacatt ggaaaaaaga ggattataat aaaaatttag
    15301 ataatgttat tgagaaaaat aataactata gagagttaat gaaaaagcaa tatggatttg
    15361 aaataaatga tgaagtagta aattacagga tagattattg tgatagattt attccaaata
    15421 aagattattt aagtaaatct aagagattaa gcttttttga tgagcttatg atattagact
    15481 caaatattta ttcaagtgga aatatagata tagactttag ggatagaggt atagttgaat
    15541 attttataga gtcattactt tatagaaatt tagcatataa tgtgaaaata aggatatcta
    15601 aagaatacaa aataagaaaa agaactatag atagaataaa aaggattgtt cttgtatagt
    15661 tttatattag taagtaataa attgtatcat aaatattgtc aaaatgaggt gaagaaggtg
    15721 aatcttacaa aagtaaaagg aaataccttt tttataaaag gtggcacgaa tacaggaata
    15781 tatgtatttg aaaataacac agcacttatg atagacccag ggttatgtgg ttcaagacct
    15841 aggagagtaa ataaagtatt agatgaaaat aatatacagg ttagatatat tataaatact
    15901 catggtcatg atgaccacta tggagcttgt aatcaattta aagaatatta taaagatgtg
    15961 tgtataatgt catctgaata cgataaatta tatatagaaa atccagaatt gttttcaaag
    16021 tatattgttg ggggaaagag caatgtattt atggataata tcttaaaaaa taaaatgttg
    16081 gatgatataa aaatagataa atctatagaa gctggaaaaa ttgaattaaa caatgaaatg
    16141 tttgatataa ttgaatttca gggtcataca cctggtagta tagctgtttt gacgaaggat
    16201 aatgtgattt ttgttggcga tttattgata ggagatgaaa ttttatcaaa atacgatttc
    16261 ttgtttctat atgatataca agaacaaata aattcattag aaaaattaaa aaatatagat
    16321 tttgagtact tagttttagg gcatggcaag caagttatat cgaaagaaga ttcttataaa
    16381 ttaatagaaa aacatttgaa tgctttaaaa aaatatttat atcaaataag agagttgctt
    16441 gtagaaccga aatctttaga aattttatta aaaaacatta taaataacaa taatttaagc
    16501 aataattata aagagtatca tttttttaaa tcttcgttag tatctgtcat aagttactta
    16561 gttgatttag aagaaatagg gtatatattg gaaaatggag aattgcttta ctatacaaaa
    16621 acaaaataaa ttatgataaa ataaagaagg tcataaaaaa caaataaagg gtgagcaata
    16681 tggaatacga aaaatggaat gagatactag caccttatga acatgcagta gaggaattga
    16741 aaataaaatt taaaaatata agaaaagaat atttagcaaa aggtgaatat tctcccatag
    16801 agtttgtcac aggtagaaca aaaaaaatat cttccataat atctaagtta aaaaggataa
    16861 atgcaaaaga tatagaaact gaaattgatg atattgctgg tataagaata atgtgccagt
    16921 ttgttgagga tatatatgct atagttgatt tgataaaagt aagaaatgat atgactataa
    16981 taggagaaaa agattatatt acaaattaca aagatagtgg ttatagaagc tatcatgtta
    17041 taataaaata ccctataaac tccattgcgg gttcaaagga aattatatgt gaaatacaaa
    17101 taagaactct tgcaatgaat ttctgggcta caatagaaca ttcattaaaa tataaatatg
    17161 accattacat acctgagact cttgctgaaa ggcttagaag agcatctgat gctgcctttc
    17221 ttttagacca agaaatgagt gaaataagag aggacataat gaaagctcag gctatgtacc
    17281 aaatgaagtc tatagctatt agagatactc taaatagaat acaagagtta tatgacttgg
    17341 gtgatacaca taaagctatg cagtaccaaa gaaaacttga tagaactgaa acagataaaa
    17401 atataactga aattttggaa ttaaaacaag aaatagatat attattagag caatataagg
    17461 atactaaggt agatgataaa gtagttcagt aaaaactact tgtagaaaat ttgctaaata
    17521 ttaatggggc tgcctcttaa tggaaaataa tttctaaaaa gagacagcct atttagtgta
    17581 attaagaaag gaatgatatt atgagtttgt atgctatagg tgatttacat ttttcaacat
    17641 cagtcaataa gccaatgaat atctttggaa gtaattggga tggtcatgaa aagaaaatta
    17701 tagataattg gaaagaagtt gttaaggaag aagatatggt cttagtacta ggagatacat
    17761 cttggggaat taatttatcc gaagctaaaa aagatttaga tataataagc aaacttccag
    17821 gtcagaaaat acttataaag ggaaatcatg attactggtg gacaaccgtt acaagtttaa
    17881 ataagctata tgaagacatg cgttttattc agacaaattt ttatgaatat aaagattatg
    17941 caatttgtgg aggtaggggc tggatatgtc ctaatgatgt taaatttgat gagactgatg
    18001 aaaaagttta taaaagagaa gaacatagac ttagactatc attagaatct gctcgtaaaa
    18061 gtggtcactc aaaaataatt gtgattactc attatcctcc tacgaatgat aaattggaag
    18121 aatctttatt tacaaagtta tttgaagaat ataatgttga aaaggtgatt tatggtcact
    18181 tgcatggtaa agaatctttt aaaatgggat tgaaggggat tcgaaatgga gtagaatata
    18241 cattagcatc ttgtgactat acagaattta atttaataaa agttcacgat taaaaaacat
    18301 aaattggtaa atactataag atacatgatt aaaaataaat atttgaggga tttcggtttt
    18361 gagaaaggag acacgactta tgaggtggat attagttttt atatttttat atcttattcc
    18421 tttagtagtg atattcagaa attacaagaa ttttaagagg tcttgtatct acagtagtat
    18481 ttatgtggta ttagctacaa caataatgat aagtaatatt tatatgagtg gacttaataa
    18541 aataaaagag tctatgtatt atcacaatta tatttctgat gagacatatg atgaaaagta
    18601 tgtatcaaat tttaaggaaa aagaaagtac tgatttaaaa caggataata aaaagaatga
    18661 agaaatatca aataataaaa atttaaacaa caaacaagat agaaatatac aaaataaaga
    18721 aagtaatgaa gaaggaagta atgatataaa agaaaagaat aattctgata gtaatgtttc
    18781 agtgaataat aaatcagaaa aacaagaaga taatacaaaa gaaaataata aatatacaag
    18841 taaagaaaaa aatagtgaag atataaaaaa aatagatatg gaaagtatag gaatttttaa
    18901 gaaagaaatt tataatatag aaagagcagc tttagttcca atgagagaat gtatacctta
    18961 cactaaggat atagcaaaaa atataactaa attatctagt ataaaaaaag atgttacaaa
    19021 tgccagaaac aagtgtaaag aagttgtaaa agaatatgaa aatatgcaga ttccagaact
    19081 ttctaaggag aagtatacag aaacattgag taatgctaga gatgacgtaa agaaagcata
    19141 tgagcttaga gaaaaatcta tggaaagtgc tttgaaatta ataaatacta aaagccctaa
    19201 atatataggg aagataacag aatatcttga tttatcagat aagcagatag aaagttttaa
    19261 aaatagattg aacgatttaa aagaagaaat aaataattca gaaacaaata atattaatta
    19321 ataagattta aagatataag ttaaaaggga gattatgatg aataaacaga gtataggaaa
    19381 actaaatatg ttttttatgg ggattactca aagaaacctt gataataggg atttttttat
    19441 tgagttagag gtgattttta aatctggaaa aaaagagttt aaaggaattg caatagagga
    19501 agataataaa tataaattta attttaaagg aaaatcagat ttatatacgt ttaatgagtt
    19561 gcttaagaat atttctaaag aggcagaaaa ttatgatgga ttagtattta aatatgttga
    19621 gcgaggaact attgttgtaa ttgaaggaga caataagaag gtaaatgtaa aatatttaga
    19681 taataaggaa gaagtaccta aaatagatga atttacggca tctcaaataa aaaacagaga
    19741 ttattatgta aaagtaggac aagcaaatgc acttttaaaa gaaattggag tacttacaaa
    19801 agatggtaag ataaaaaatg ataaaataag aaaatacaat cagatagacc attttgttga
    19861 attgatagat agtattttaa aagaaataaa ggataaagac tgtataacta tcttggattg
    19921 tgcttgtgga aaatcttact taagctttgt cttaaatttt tatataaaag aagtattgaa
    19981 gaaaaactgt tattttatag gaatagatta ttcagatgta gttatagaag catcaaaaaa
    20041 tatggctaaa aacttgggtt ataaaaatat gtcatttata aaagaggatt tgactaatta
    20101 tacacctaat agggatgttg accttgtaat aagtctacat gcttgtgata cagctacaga
    20161 tatggcaata ggtctaggaa ttagagcaaa atctgaagct atagtagtag taccatgttg
    20221 tcataaggag ttattagggc aatatagata tgaagctatg gaaccaatat taaaacatgg
    20281 tgtatttaaa gctagatttg cagatttgat aactgatggt ttaagaacat tgttattgga
    20341 gggtaatgga tatgatactt ctgtagtaga atacatatct ccacttgata caccaaaaaa
    20401 tttaatgata agagcgataa aaactaaaac gaataatgac aaagcattaa aagaatataa
    20461 agaactgaaa tcacaatttg gagtggaacc aaccttagaa aaacttatat attagttaaa
    20521 aatgtaaaat ttacgaaaag cttgcttaaa tatgcaagct ttttggtatt atataggtat
    20581 agtcatatcc agagttttga tttataatta aagctgtgct ataatatttg attaatcaaa
    20641 tcaataaatg ttacatggag gtaaaaatga acaaattgaa tatatgtatt gatatagatg
    20701 gaacaataac aagcccttat cattttcttc catacctaaa tgatatcttt aataaaaata
    20761 taactgaagc agagtgtata acacataatt gggaagaatt gtatggttcg ggaataagag
    20821 atgtgtatgc ggagtttaat aataagtata tacactcata tgatgaagca aagattgtag
    20881 agggtgctac agaggtaatt agcacattat ctaaaggaca taatttatct tttgtaacgg
    20941 ctagacatga gtgtttaact gatgtaacta aaaattggct atcgagacaa ggattttcag
    21001 atatagatgt ttacttatta ggaagtgatt ataaagttga aaaggcaaga gaactgagtt
    21061 gtgacatatt tatagaagat aatcctttaa actcagtgca actagctgat gatggtgtta
    21121 aagtaatact tctggatact aactataata aagatgttaa acatcaaaat atagttagag
    21181 ttagtaattg gatagatatt gaaaagatta taaaacaagg tatttaaaga tttgtagatt
    21241 gaaaggggga gcatatgata gatttaaaaa ttgaaaatag aaaaggaaaa atccatgtaa
    21301 agagcagtga aataaaagac attttagaat tacgtccaga cttcgaatat gtacaagata
    21361 tatcaaatac tataaatcaa gaaaatatat tggtatttga ttgccagttg acaggaaatt
    21421 cttttgatat ggatgattta gatatagaag aaatattgga agagttaggt gaagaaatag
    21481 atgagtctta tttcagtgtt ttatttgaag atgtaagagc gtatttaaaa gatgctactg
    21541 atgagataga agcagactta caggataatt acttatttga taatttgaga tgttattttg
    21601 atatatacaa tataaatcaa gaatttacag attttaagtt tgtattttta gtttctttta
    21661 aagatataaa aatttcttca ttagcaaacc tagctaagat tgtttctaag agacagctta
    21721 taggtgcatc taagttttat agttaaaatg aaatattatt ataataaaag ctagtaacct
    21781 tgtaaaaata taggttacta gttttttatt tattcatttt cacaaaattt cagaaaacta
    21841 tttacattct aaaaaaagta atgtatatta aacataatgt acattataat ggataaaaag
    21901 tttctattaa attactaagt ttatttaata atcaaaataa tttatcttat attagtatgt
    21961 attagggggt atttatgcaa agtttaaatg aaataattgc tgaaaattta aaaaagataa
    22021 ggaaagaaaa acatcttagt ttagacaaga ttgcacaact tagtggtgta agtaagagta
    22081 tgctatctca aattgaaaag tgtgaggtaa atcctactat atctactttg aagaaaataa
    22141 ctaatggact aaaaatctca tttacatcat tgatggagag gcaggaaagt gacatagaat
    22201 tgattcaaaa aagtgatata gaccatttta ttgaagataa tggaaagtat ataagttatc
    22261 caatatttcc atttgactcc aaaagaagat ttgagatatt tatgattgaa atagaagaag
    22321 gtggaaattt agattcaaat gctgaacttc caggaacaca agagtttata actgttttta
    22381 gtggcgaagt aaccattaaa ataaatggag aagattacat tgtatctagt ggaaattcta
    22441 taagatttaa agctgatgta tcacatatat atagaaactc aggcaaaggt atagctaaaa
    22501 tgagtatggt agtatattat atataaataa tttaatggaa ttagagtgat atttagatga
    22561 ttttaggtat gaagatatat tatatttaag ttataggagt gtgatttatg gatgaataat
    22621 aaaaaagcag tttttttctt ttgtagtgat ttaaagaaag acccagtagc cagtaatgtg
    22681 ataaaatgtt gtgaagaaat aatggattta aatcaaactg atattgttat tgatgataat
    22741 tttgtattag agtttaagga taaaaacaat aacttatttc attttgtaaa aacaaaagat
    22801 gtaataagtc ataattataa atattatctt ccaattttaa atagacattt tagagattat
    22861 gattttgcag tagttgtaaa ttggcatgag ggagaaaatg ctccagaaca tatcctaaca
    22921 gctcatacaa ttggagatgt tccaacagga gaatttggaa attcaaatcc aagatacttt
    22981 aaaaatctaa tcaatgctat tgaggatata aaaaatgaaa atctacttga tgaatttact
    23041 acattaacag aagcaactca ctggtctgga acaacatatg gtgaagaatc taaattaatt
    23101 acagaatatt ctgtacctat gttggatatt gagataggaa gtagtagtga tagttttaat
    23161 aatagtatag caatacaggt attggcaaaa tcattaatta gagtatttga ttgtgatgaa
    23221 cctttgaaga ctttgttatg tgttggaggg gtacattttg agaaatcttt ctcagatatt
    23281 ataaaaaata aagaatataa catatcaatt ggacatgtat tgccaaatca atggattgta
    23341 agtggaatgt atgatgatga atctggattt gggaaactag aaaagtgtat aaatagtatt
    23401 gaaggaggaa ttgattgtat agtttttcat gataaattaa aaggtaccta taaagagcaa
    23461 tgccgtaaac taggtgaaaa acttaatgta cctgtattta aacataaaat attaaaaaat
    23521 ccaaaagact tacctatatg gtagttttat acagttaata taacaataag tcggattaat
    23581 ttttaagcga ggtcaaaaat gatattatct tattttgcat tattaattag agtagtgcta
    23641 ttagcttttg aaagagtagt tgtaagatta ttaggtgatg aagaaggaga tatttataaa
    23701 aatatagctt catcatttct atttttcttt atagggggag tatgtttact tccatttagc
    23761 ataatggata gagttagtga ttggtcgttt ttaataccat gttatataag tagtttagtc
    23821 tattcaattg gttcagtagc gtatgtaacg tctttagcaa ctggggaagt ttcacttgtt
    23881 actccaataa atagtttaaa ttcattgttt ttactgcttt tatcagttat atttttaggt
    23941 gaaggtttaa gtttagcaaa agttgcagga atcataatta tgattggtgg agtgtttata
    24001 ttaaaaaaag taagctcttt tttaaagaat gtctctgtaa tttttaacaa tttaccatgt
    24061 agattaatgt ttttatacat atttttgcag tcagtaggaa gagtattgga taaggctttt
    24121 tttgtacaag tatctcctat attatattca actatactgt attttttcgt aggtttaaat
    24181 ttatttatct ttttagcatt taaaaagaaa caaaaaataa tttgtgagat ttttactaat
    24241 aaaaaggggc tttcaatttt aagtggtgct attaatggat attcttattt agcattatta
    24301 attgctttaa ataatctaga acttagtata gcagagccat tttctcaagt atctatgata
    24361 ataactttaa tacttgctca ttttatattc aaagaaaata taaaagaaaa aataccaggt
    24421 tcaattttga tactaattgg tgggtggcta ctgttacttt aattgacata aaaaatgaat
    24481 tataaaggtg ctattagtta gtatcagaaa atttaaaaaa gtattgacaa ttcttttagg
    24541 caaatgttaa tataataata caaaatagta taaatagaag acatgttact ggtaatgcag
    24601 gcaggatcga atatattgct ttgatttttc aaggtgttta tataggttct gccttttttg
    24661 catcttgaat ggccattcat aaaaaattat ggagaaggag attacaagat gaattataaa
    24721 aaaactgcca ttgacattat tgaacaagtt ggtggttcaa aaaatatttt agctttagaa
    24781 cactgttcaa ctagactacg ctttaagttg attgatagaa gtaaagtcaa tcaagatgct
    24841 ttaaaaaaag tacaaggtgt tatgagtata gttaatgggg cacaatttca agttgtcatt
    24901 gggaatagtg ttgttgatgt gtatgatgaa attttaaaag tttgcccttt agatacaaat
    24961 gatagtactg taaaaactga agaaaaaata aacttaggaa aaagatttat agaatttatt
    25021 attaatattt ttcaaccact gatttttgct attgcaggag ctggtatttt aaaatcactg
    25081 ttgatgttat tagcaatgtt taatatttta aaatcagatt ctactatata tacattatta
    25141 gttgctattt cagatgcaac attctatttc ctacctctta tggtagctgt aactactgcc
    25201 aatgtattga aatgtaatcg tttagtagct attgccgctg taggatactt attgcttcca
    25261 gcaacaacaa ctgctttaag tgaaggtgtc gaactatttg gatttacaat tccaaacatt
    25321 gcctataatg cacaggtttt cccagctatc ttatgtgtgt ctttcttggc aacaatggag
    25381 aaaattttta acaagtattc accaaaacca attcgaactt tctttgttcc tatgatgtct
    25441 ttggcaatta ctgttccaat tactctaact attttaggtc ctttaggata taacgttggt
    25501 acaattttta caaccattat tttattctta tataacaaaa tgggattctt agttgtagga
    25561 ttacttgctg cttttcttcc atttatggtt gcaacaggaa tgcacaaagc actaatacct
    25621 tattcaatta atacaattgg aaagttgggt tatgaagctt tatatatgac atcttcatta
    25681 gcccacaata tttctgaaag tggtgcttgt tttgcagtag ccttgcgaac aaaagatgaa
    25741 acattaaaac aaacagcatt atctgcagga atttctgcct tgatgggaat tacagagcca
    25801 gctttatatg gtattacttt acaacataaa agagcaatgt taggtgttgt attctcaagt
    25861 gcaatttctg gtaccttttt aggattattt gcagtaaaag gatttgtaat tgttggacca
    25921 gggcttgcca gtatgagtat gtttattgac ccaaacaatg gaaacaattt gatttttgca
    25981 agtattggtt ttgttattgc aatattaggc tcgtttatta ttacactgat tatttggaag
    26041 gaagatacaa atacagtaga gtcatcaatt ggagatgttg ttgaagaaga gctagaagaa
    26101 gaaaaagaag taaatatctt agtaactcca gtagaaggaa aagtaataga tttaagtaag
    26161 gttaatgatg aactatttgc aagtaagact ttgggagatg gagttgcaat tataccgacc
    26221 aatggaaatt tatacgcacc ttgtgattca gaagttgtga tgttatttga aacaaaacat
    26281 gcaattggtt taagaacaaa aaatggggct gagattttga ttcatattgg aataaatact
    26341 gtaagtatga atggagatgg atttaaaaca tttgttaaga caggcgataa tgtaaaagaa
    26401 ggagatttat taattcaatt tgatttagac aaaatctcac atgctaattt agattcaact
    26461 gttatgattg taaacaataa tggagcagat tatgcttata aagtgttaaa ccagtcttat
    26521 ggcaatgtaa aaaaaggaag tatcttgttt gatgtaaagg gaggaatata aatatgagaa
    26581 agtttccaga aggatttctt tggggaggag ctacagctgc taatcaattt gaaggtggtt
    26641 ggaatttagg tggtaaaggg tggtctgtat cagatgtagc aaaggcacac tttgatgtag
    26701 atgtgaaaga ttataaatct aataatgaaa tcacaacaaa agatatagaa gaaggattag
    26761 cacatcctga agatgaagta aattatccaa aacgtcatgg tagtgatttt tatcatcact
    26821 ataaagaaga tattgctcta atggcagaaa tgggatttaa gacatatcga atgagtattg
    26881 cttggtctcg tatttttcca aatggagatg ataaagagcc aaatgaagaa ggtttacagt
    26941 tttatgatga tgtatttgat gagttgataa gttatggaat tgaaccactt gttactatgt
    27001 cacattatga accaccatta aatatcgttt taaactatga tggatggtat tcacgacaag
    27061 ttatcaatat gtttgttagg tatgtagaaa ctatctgtga aagatataaa aacaaagtga
    27121 aatattggct tacatttaat gaagttgatt ctatgattcg tcatccgtac actactggtg
    27181 gtttagttcg agaccgtttt aaggataaaa actttgagga agttattttt caagctatgc
    27241 atcatcaatt tgtagctagt gccattgcaa caaaaatttg ccatgagatt atttcaaatt
    27301 caaaagtagg gtgtatgtta acaaaattaa cgtattatcc atatacatgt agaccagaag
    27361 atgttttggc aactcaacaa aaaatgagaa gtatttatgc atatagtgat acgcaggttt
    27421 ttggtgagta tccagtatat ttactgtcgt attttaaaaa taataacatt caaatagtaa
    27481 aagaagaaca tgatgatgaa attatgaaaa agtatccagt agattttatt tcattctctt
    27541 actatatgtc ttcttgtgag gcagctgata caactggact agatattaca cctgggaata
    27601 cgttattagc tgtaaaaaat ccttatttag aaatgagtga atggggttgg caaattgatt
    27661 caataggtct acgtatatct ttgattgaat tatatgatag atacagaaag cctttattta
    27721 tagttgaaaa tggattggga gctaaagata ttttaactga agataaaaaa gttcatgacc
    27781 aatatagaat taattattta aaagaacact ttaaatgtat gttagatgcg attattgaag
    27841 atggtgttga actttgggga tatacttcat ggggttgtat tgatttggtc agtgaatcaa
    27901 cgaaacaaat gtcaaaacgt tatggatata tttatgtgga tgctgatgat tatggaaagg
    27961 ggacatataa tcgatataag aaagatagtt tttattggta taaaaaggta attgaaagta
    28021 atagtataga ttttaaatga ccaatagaag gtgagcaaat aaaatgaact acataataaa
    28081 gaaagttttg aactcgagtg ttgttttagt tcatgacaca aaggataatg aatttatttt
    28141 gctagggaaa ggaattggat atggtaaaaa aacaggagaa tatatttgtg gttccaatga
    28201 taatcaaatg tttgtccctg ttgaaaatac aaaatcaaaa caatttttag aattaatgga
    28261 tgatattcct gtcgacattt taaagataac acaggaaata atagtagagg cagaaaagtt
    28321 attgaactca tcttttaata aaaacttata cttaatatta gcagaccatt ttaactttgc
    28381 aattgaaagg atgcgtaaag gaataaaaat tacaaatcga gtattttggg aaataaaaaa
    28441 ttattatcca aatgagttta aggttggaat ggttgcaatt tatatggtag aggagagatt
    28501 gggcattcaa ttaccagagg aagaagcagc taacattgct ttccattttg caaacgctat
    28561 ggcttcagat ggaaattcat atgatacgat taagtatgcc aaagtaattg gagaaataat
    28621 taatattttt attttttctt tgaatagaac cttagataaa aagagtatgc attatatgag
    28681 atttataaca catataaaat tttttgtgga aagatttttt tcagatagta tgttaagcag
    28741 tggtgatgat ttattattta atcaaatgaa aaggtcaaat ccaaaagaaa tgttaattgc
    28801 attaaaagtt agagattttt tagaaaagaa atatggaaaa aagttaacaa atgaggaaat
    28861 agctttcttg gttgtccata tcgcacgagt taagcaatag agtgaattac taatattatc
    28921 ttatacgtct acatggtaga aaattgaaag aaggtaaagt atataaagaa actgagacat
    28981 attattttgt tgttcattat gtttatttta tg
//
LOCUS       DACXMZ010000021        28783 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.021, whole genome shotgun sequence.
ACCESSION   DACXMZ010000021 DACXMZ010000000
VERSION     DACXMZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28783
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.021"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(448..1023)
                     /locus_tag="JD016_03730"
     CDS             complement(448..1023)
                     /locus_tag="JD016_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HAU4998742.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            1283..2575
                     /locus_tag="JD016_03735"
     CDS             1283..2575
                     /locus_tag="JD016_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAU4998743.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            2680..3282
                     /gene="yedF"
                     /locus_tag="JD016_03740"
     CDS             2680..3282
                     /gene="yedF"
                     /locus_tag="JD016_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAU4998744.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            3355..3618
                     /locus_tag="JD016_03745"
     CDS             3355..3618
                     /locus_tag="JD016_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HAU4998745.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            3633..3818
                     /locus_tag="JD016_03750"
     CDS             3633..3818
                     /locus_tag="JD016_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAU4998746.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            3921..5117
                     /locus_tag="JD016_03755"
     CDS             3921..5117
                     /locus_tag="JD016_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAU4998747.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            5331..5609
                     /locus_tag="JD016_03760"
     CDS             5331..5609
                     /locus_tag="JD016_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAU4998748.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            5646..6080
                     /gene="ssb"
                     /locus_tag="JD016_03765"
     CDS             5646..6080
                     /gene="ssb"
                     /locus_tag="JD016_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAU4998749.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            6099..6326
                     /gene="rpsR"
                     /locus_tag="JD016_03770"
     CDS             6099..6326
                     /gene="rpsR"
                     /locus_tag="JD016_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAU4998750.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            6469..6789
                     /locus_tag="JD016_03775"
     CDS             6469..6789
                     /locus_tag="JD016_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998751.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            6797..7753
                     /locus_tag="JD016_03780"
     CDS             6797..7753
                     /locus_tag="JD016_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HAU4998752.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            7764..9758
                     /locus_tag="JD016_03785"
     CDS             7764..9758
                     /locus_tag="JD016_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAU4998753.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTAHIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            9759..10208
                     /locus_tag="JD016_03790"
     CDS             9759..10208
                     /locus_tag="JD016_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAU4998754.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            10227..11555
                     /gene="dnaB"
                     /locus_tag="JD016_03795"
     CDS             10227..11555
                     /gene="dnaB"
                     /locus_tag="JD016_03795"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAU4998755.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            11760..12068
                     /locus_tag="JD016_03800"
     CDS             11760..12068
                     /locus_tag="JD016_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998756.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            12150..13439
                     /locus_tag="JD016_03805"
     CDS             12150..13439
                     /locus_tag="JD016_03805"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAU4998757.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            13765..13840
                     /locus_tag="JD016_03810"
     tRNA            13765..13840
                     /locus_tag="JD016_03810"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13798..13800,aa:Lys,seq:ttt)
     gene            13847..13921
                     /locus_tag="JD016_03815"
     tRNA            13847..13921
                     /locus_tag="JD016_03815"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13880..13882,aa:Glu,seq:ttc)
     gene            13926..14001
                     /locus_tag="JD016_03820"
     tRNA            13926..14001
                     /locus_tag="JD016_03820"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13959..13961,aa:Val,seq:tac)
     gene            14035..14109
                     /locus_tag="JD016_03825"
     tRNA            14035..14109
                     /locus_tag="JD016_03825"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14067..14069,aa:Thr,seq:tgt)
     gene            complement(14492..15484)
                     /locus_tag="JD016_03830"
     CDS             complement(14492..15484)
                     /locus_tag="JD016_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAU4998758.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(15493..16551)
                     /locus_tag="JD016_03835"
     CDS             complement(15493..16551)
                     /locus_tag="JD016_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAU4998759.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            16733..18139
                     /locus_tag="JD016_03840"
     CDS             16733..18139
                     /locus_tag="JD016_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HAU4998760.1"
                     /translation="MVLIIGCFIEKDDSLETYKEKTNSKINQYNMDVIFDDETKRLMC
                     NQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEMTRAYPNGFNEGYIDIKNIL
                     NNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYNVKIPNSIGRFGYGENTINV
                     TNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLLIPNKYKIAHTGKLIDDKHD
                     NKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTYNLNKKLSKKATEVAKDSIN
                     IFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLYNEKNEFLLEYVIAHETAHQ
                     WWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMANKLIKTMEIQTKSYLSENIF
                     KPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFETLNEYYNKYKHKNANGRAFV
                     ELWNSKGVDINKIISECK"
     gene            18241..19038
                     /locus_tag="JD016_03845"
     CDS             18241..19038
                     /locus_tag="JD016_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAU4998761.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            19051..21153
                     /locus_tag="JD016_03850"
     CDS             19051..21153
                     /locus_tag="JD016_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAU4998762.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            21221..21700
                     /gene="rlmH"
                     /locus_tag="JD016_03855"
     CDS             21221..21700
                     /gene="rlmH"
                     /locus_tag="JD016_03855"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAU4998763.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSRMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            21767..23458
                     /locus_tag="JD016_03860"
     CDS             21767..23458
                     /locus_tag="JD016_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998764.1"
                     /translation="MDRLDIFIENYDFKEYLEKLPRELIEENYKDFVIEFWKNPSVDI
                     IKLKSKYSIKNVVSISSILMQNPIQEYYEFLEDIWKNNIKIDELINKYLITATRMDQI
                     KFPLIYIDEDSFCPYCLGNKFNIINNIKQQDIKVECEKCGEAISYFIHKEEVDNIIEE
                     EKRKKELFIRTLENVYNKINKIKCHRCQSDLYVCINDKIMEYNIRCDRCNSQYESVEI
                     AQEQYRLWQQRSAMMANIKNKENELLLKTLSIKDEKDIKFKLEDIITESESIDVISDL
                     VDMQDRGENIFKQLFNNYVRLGRLDRRLLVTVCKDCKQNENTYTWERGKEHLINMRGI
                     TYSEAVVYELMEKTKLLPIRAIIRRLMEKKLLYCDERSNNLQVHPVLIENLAKLESIN
                     KSQNINNQVAYLIFNKQKYICYHCGESGRPLKIGYLTIDKNINDLSSLVGICDSCYED
                     ITEDEIIIDNIFCLVDERNNELGEAWKFLIQYDSSFRNNTIAEVEHARLYDMYDEDNL
                     IRAYANTLYKQTKDGFANEKVFFAYARAILKNSKGQVSVLQIAKEKFKLEKWLNG"
     gene            23690..25162
                     /locus_tag="JD016_03865"
     CDS             23690..25162
                     /locus_tag="JD016_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998765.1"
                     /translation="MSHSKLSDHKLKKGKFIAPFNEFMTPLNSNESWCLGRLPEYIWI
                     GLLINKYGRKKGMEIIYKIIVKLHSIESDIVTPRLSEILSMEDVNQDEFFSYILTIVE
                     KEALSPLTLIFTYSNYPIFVKYFAEPSIKIEDRVTYLDETLRKAMHHQSEFATDIRFI
                     VLYYFCMSGKLVLQKEQINNLLEYPNLEHTDEKMRMLRPFIRAAELMVLNMEKKSDWY
                     LDIFWGRISVMTECKLYATKFEDETANTDLYMEQLHEVFVYLSDLLCKVNPLDEKMLV
                     LLGIAIYSYKRLREVVEHKLFNSISGRGSVRVLIENYIMMKYLVKIEAQHENIWSEYQ
                     FYGIGQYKLIVARYREQEQDLKNSHVDYKYIEALVNEFVIEETIDMDTKYFDKQNIRS
                     KAEIVGEKELYGLYYDYDSAYEHGLWGSIRESSMLKCDNPAHHYHCIPDVDNNQNLKS
                     VWHDCVNIMEKTLLFLDSVYGIPQHLLLEVIKNGKEFFTK"
     gene            25392..26201
                     /locus_tag="JD016_03870"
     CDS             25392..26201
                     /locus_tag="JD016_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998766.1"
                     /translation="MGDIHIVDDSLCKYSKTVLVLSNQEFAKVLKDQIISCIDDNIKI
                     LSNYSFAMLILPMNLLYGNDMKIIHEYSNNIFLNFFSRKDLNLDTYFEEFNSIEDIAL
                     ALNPGIESYIVFDEGEDLTKNLEERFKSFIDKGKVPLPAHKSDAFKFWLIIYGFITQA
                     LDIILSCKGFNLTPYLRYRVTLQYVLLLGDNVFDIEDLSNMLLKCKVAHVLYAIFDKS
                     EIKNISFVQFYKKLSEYKFSYKVEDDIKENININQIAEILNKNITLFLNYI"
     gene            26341..27624
                     /locus_tag="JD016_03875"
                     /pseudo
     CDS             26341..27624
                     /locus_tag="JD016_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019555567.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            27940..28251
                     /locus_tag="JD016_03880"
     CDS             27940..28251
                     /locus_tag="JD016_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU4998767.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            28299..28616
                     /locus_tag="JD016_03885"
     CDS             28299..28616
                     /locus_tag="JD016_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAU4998768.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
BASE COUNT        11508 a         2992 c         4874 g         9409 t
ORIGIN      
        1 tttattcaaa aactataaaa gaacattaac ttttttagaa gtagaattta caactaaaga
       61 ttgtaatatt gctattgaat ctaagcatta taaaaaacat tggatacaat ttattggtat
      121 agcattttca aaatgtcaat atcacttgtt atattactgt aaattgtata gattatctta
      181 gccttgaaag tatttgtgta tatatggtga ctatgataat tgacaatcta gtaaataaga
      241 taaagataac attagtagga taaaatattg gtatataaaa gatctaaata gaacctgaaa
      301 ggtgttgttt caaaattgat tttttataat caaattttta tttctatttt tatgtttttg
      361 tatgtaaaaa agaaaggaag gagataattc aaatttttat tttgaaccat ctccttctat
      421 atgataattt ataattgttt taagaagtta ctttaagtaa tctccttctt caagaactat
      481 atgaccactt tcgtaattag aaccatcaac agtaattttt atttgttcaa cattcatatt
      541 ttttatgtaa gtacgagcaa ctgaatcaag cattaaagtt tctgcatcac tacctaagtt
      601 tgactttgta aagtcagagc taacatctaa tataccagtt tttacaccat caatgtcttt
      661 tgtagtaaat gaatttaatt ttgcagattc tggaacaact ttaagttttt gaagttcttc
      721 aaataaagtg ttctcatcaa ttttatttaa atcaacttta ttttcaatta aatctgtctt
      781 atctacatca aaagagtaaa tagtagctga ttgagtttta gtttcttctt ttggagtagg
      841 tttagctttg ttattaggaa cctctttttc agtattgttt tcttcttttg gttgctcaac
      901 atttgtattg ctcttagttt cttttttaga tgaatcactt ccattttgag cattagtaca
      961 accaactgcg aatatagata tagataatat acttaacact aacattattt tcttattttt
     1021 cataaaaatt tctcctctct taaaaactat tcttatatat aattatagtc gttatgagca
     1081 aatatatgaa tacaaaatag taacaaattt aaaaaatgat ttatttaaat taataaaact
     1141 caaaataaac taaattttag tcatttttat gggaaatcat ttaatgtaca gtgaaaatat
     1201 tgtaaaataa aagtagtgtt tttaaaataa atataagtta taattatatt ataaaaaaat
     1261 gtcttcaaaa ggtgggaata gtatggaaat tttgagctta ggagaaaaga ttaaaaaatt
     1321 aagaaaagaa aaaaacatga cattaaaaga attagctgga gataggataa cagcagcaca
     1381 aataagccat atagagaggg ataaatctca tactagttat gaactgttgg aatatctagg
     1441 cgataagcta gatgtaagtg tagaatactt attagagacc aaggaaatgc aatcaaaaaa
     1501 gataactgat aatttaatac tacagagcga aatttatata aaagaaaatg aattaaacaa
     1561 ggctgaggac caaataaaag aagttttaga tatatgtaag agatacgatt taatggagaa
     1621 ctatggcaga tgtaattttt taatggctaa tattaatttg aaaaaatcta attataatga
     1681 tgctatagaa aactttgaaa aggcattgta cttctttata aaaaatagtg atagtgaaaa
     1741 tatacttaag tgttatttaa acatagggaa aatatatatg caggaagact tttataaagg
     1801 tgctataagc cactttaatt ttgcagagga aattttgtca gagaataaat ttgaggatgt
     1861 taacatacac aaggaactat atagtaagat ggcatattgt tacataaaac tagataatcc
     1921 aaatatgtca ttgaagtaca tagaaaagat aaatgaaata gataatagaa atgatagaaa
     1981 agaagatgtc gatattttgg tgttaaaagc aaataatatg cttgagattg gaaaatatga
     2041 agaatcaaga gattacttta aaaaagcact aaaaatactt gagagtgagg ataataaaac
     2101 tggattagca aatgtgtatt taacaatttg tgatgtttac agaaaaatag gtgaaacaga
     2161 tagagtactt gaatattcac aaaaggtata tgacataaag aaaaatgatg aagatgaata
     2221 catgatgtca ggattattta agataataga agcatatata gataaagaag attatgattt
     2281 agctagaaaa tattgtaaga tagccttagc ttcatcaata aaaaataaaa ataaattcaa
     2341 tgaatataaa gcattgagat tctatgctaa tatgtataaa aatcagaatg aagtaactct
     2401 tgcaattgaa tatctgatta aatgcataaa aatagtatca gatttaggca acaatagaat
     2461 attggccaat ttatatatag acttaggtca attatattca aatatatcaa aagaaaaaga
     2521 gttagaatat tatcaaaaag gtgtatttat gtacaaaaat ttagaaataa tgtagggtaa
     2581 ataaacctat aagggtatag aaacaatcta taacaagcaa gataatatct atgataaaat
     2641 ataaattgta tgaatatttt taaggagatg aatcagacaa tgagtataaa aatagatgcg
     2701 agagggcttg cttgtcctaa accagttata aatacaaaaa gagaattgga taacctagaa
     2761 gaaggtgttg tagttacaat agttgataat gaaacagcta aggagaatat attaaaactt
     2821 gctaaatctt taagttgtga agcaaatatt gttgacgaaa aagagaattt tatatctata
     2881 gaaattagaa aaggtaaaaa tatactagat aaacaaaatg ttgtagataa taaagaaagt
     2941 gaattagaca atacatgtat atttatatct tcagataaga tgggacttgg aaatgacgaa
     3001 ttaggaaaag tgttaataaa aggatttata tatacattaa cagaatctaa accttatcct
     3061 aagtatgtac ttttagttaa tggaggagta aaattaagtg ctgaaaatga agaaaccata
     3121 gaaaatctaa aaatacttga gaatatgggt gtagaagttt tatcttgtgg tacttgttta
     3181 gattattata atttaaaaga caaattacaa gtaggtacag ttacaaatat gtatactata
     3241 gttgagacat taaagaatgc atcaaatacc atttctatat aatgtatgta tgtttaataa
     3301 tataaaacta acaataaaga ttaactcaaa tagatagtca gaaaggatta agtaatgaat
     3361 caaatgtata tagtgtcatt taattcgaca catcatgcga taagatcaga aaagttattt
     3421 ggtgaaaata gtctaaaagt aatggctctt ccaacaccaa gagaaataac agcaagttgt
     3481 ggaatatcaa ttaaattttc ttttgaagat atggaaaaga taaaaactat tttggttgaa
     3541 aataatgtag atataaaagg aatttattgt ataagtaaat tagaaaatgg ttcaaaagaa
     3601 gtagagaagt tagattagga ggctaattat ttatgccaat ggatttaaaa ataggtgata
     3661 ttgttgaatt aaaaaagcaa catgcatgtg ggtgtaaaga gtttgaaatc ataagaacgg
     3721 gaatggatat aaagataaaa tgtactaaat gttcaaggct aataatgctt gatagagaga
     3781 ccttagaaaa gagagtaaaa aagctaataa aaaagtgata aaaatttaac acttaaaaaa
     3841 tgtcaaatac cgtatattaa tatccaaaat tttggtataa taagaattga agaaaacatt
     3901 ttcatggggg tgctttaatt atggcagtta aatatgcaaa aagaatgcaa ggattacaag
     3961 gatcagaaat acgtgagctt ttaaaactta ctcaacaacc acaaataata tcttttgctg
     4021 gtggaatgcc ggctccagag ttatttccag ttgaggaaat gaaaaaagta tcagttgcag
     4081 tgcttgaaga aaatggaaga tcagctatgc aatatactac aacagaagga tatgaaccat
     4141 taagagaaaa aatagcagct agaatgaatg ataaaaacaa aactaatgta aacaaagatg
     4201 atatattagt tacaagtggt tctcaacaag gtctagattt tgcaggaaaa gtattcattg
     4261 atgagggtga tgtaatttta tgtgaaagtc catcttacat aggagctata aacgcattta
     4321 aatcttatca acctaaattc atagatgttc caacagattc tgatggaatg ataatggaag
     4381 aattagaaaa gatactagaa acaactgata gaataaaaat gatttacgta atacctgact
     4441 tccaaaatcc aacaggaaga acttggccac ttgaaagacg taaaaaattc atggaaatag
     4501 taaataaatt tgaaatacca gtaatagaag ataatccata tggagattta agatttgaag
     4561 gagagacttt accatcttta aaatcaatgg atacaaaagg attagtaata ttcttaggaa
     4621 ctttctctaa aatattctgc ccaggataca gattaggatg gacttgtgct tctccagaaa
     4681 ttttatctaa gtttaacttt gcaaaacaag gtgcagactt acaagcatca actatatctc
     4741 aaatggaagt aagcaaattt atggatatgt atgacctaga tgcacatgtt gacaaaataa
     4801 aagcagttta tgttaaacgt agagatgtaa tgcttaagac tatggaagaa gagttcccag
     4861 aaggattagt atttactcat ccagaaggtg gattatttac atgggtagag cttccaagta
     4921 atttaaatgc taaagaatta atgccaaaat gtttagataa gaatgttgct tatgttgctg
     4981 gaggaggatt cttccctaat ggtggtagag aaaatacttt cagacttaac tattcaaata
     5041 tgccagaaga aaaaataata gaaggtataa aaaatatagc agctgtatta aaagaagcta
     5101 tgggtgtaga agcttaatta aaaataataa cttatatata atacaaggga tggttgttgc
     5161 catcccttgt ttgtttgaaa agtatataat tcaccttata ctaaactttg tccaaaattt
     5221 aaattgacag aaataagaat aaatgataaa atgtgtaggt atgagatttt agtctcattt
     5281 ctctgctcta agagtgtaga gccgttaagt ccaaagggag gtgaattata gtgagaaatt
     5341 atgaattagt ttatgtggta aagccaaact ctgatgaaga agtaagagag gctatactaa
     5401 acaaagttaa ggaagttgtt gcaactgacg gagaaatagt taaagttgat acttggggaa
     5461 ctaaaaaatt agcttatcca atagctaagt ttacagaagg tttctatgta ttagttaact
     5521 tcaaatcagc agtagacgtt cctaaagaga tagacagaaa cttaaagata aatgaaaacg
     5581 taataagaca catgatagtt gttgcttaat aactggtttt attataaaaa ataggtggtg
     5641 tttttatgaa tcaagttgta ttagtgggaa ggttgactag agaccctgaa ttaagataca
     5701 tcccaggtac aggaacacca gtggcatcct ttacaatagc cattgataga gactatgtaa
     5761 aaaaagatgg atctaaagaa actgatttta tacctgttga agtaatgggt aaatctgctg
     5821 aattttgtgc taactacata actaaaggta gattagtagc acttcaagga tctataagag
     5881 tagacaatta tcagacacaa tcaggtgaaa agagaacttt tacaaaagta agtactagat
     5941 cagtacaagc tttggatagt aagaataaat cggaaaattc atatagagag agtgctccag
     6001 cttttgagcc aagctttgaa cctcaaggtt tagacccaca aggtttccaa gctatagatg
     6061 atgacgatat accattttaa aaaggaggga aaaaagtcat gatgaataag aaaagacgta
     6121 agaaaaaaag agtttgtcaa ttttgtgctg acaaaaacgc taaaatagat tacaaaagta
     6181 ctcaaagatt acaaaagtat ataactgaaa gaggaaagat attaccaaga agaatatctg
     6241 gaacttgtgc taagcatcaa agagaattaa cagttgctat aaaaagagcg agaaatatag
     6301 cacttttacc atatacatta gactaaaaaa atgagccttg tgctcatttt ttattatatg
     6361 tatttatata tctaaatgag gcgagctata aagctcgttt ttttgtttat atacttaaat
     6421 atattattat ttatgatata atattatata ttaaggaggg ttgtaaacgt gaaaatagat
     6481 aaaggttctg agataacgag aaacataaag ataatagaat ggatgaaaac agaaatatta
     6541 atgagtgtaa gtgatttgtt taatttattg tttaaagggg taaaaccatt agatgaagca
     6601 attcaagata ctttagcaaa tataataatg ataacatatc tcttagcaaa gagattagga
     6661 attagtttta gagatgtgga ttataaagtc aaggaaaaaa taaaaattgg tatagatgag
     6721 gaccatagcg ttgaaagatg gtatggagat ttatctaatt taaaaaaaca tatggataat
     6781 agggagtgat attaaattga ataataaaat tagactatct aaggcaatgt ctataatagt
     6841 tttagctata ataatcgctt tagtaatagc ctatattcca atgctaggaa tatttagtat
     6901 gttagcagct gttccatatg taattatagg tgctattact gatagaaagt attctcttat
     6961 atctattttg gttacatttt gtgtactgat tctatttgca gacatttctt atgcattaaa
     7021 tatatgtata atgtattctt taccaggtat agtaatagga aagatgctaa agagaagctt
     7081 tgaacaagag gacagtaata aattcgaacc gatatatggt ggaaccataa tatttgtact
     7141 ttcaatgctt gtatattttt ttgtattaaa gacattttta aatgtgaatc tattagatga
     7201 agtttctaag atgatatcag aaattgtaaa tattcaaaaa aatagttttt ctgcaactga
     7261 acttaaagtg tttgataaaa tgaagccaga tgagattgta agttatttta caaatatgct
     7321 accaatgatg ttatttttac aagggttgtt atcagcgttt gtcacatatt atttgagtgt
     7381 attttttata aaaaggatta caaagatgaa tataagattt cctaagtttg ctgattttta
     7441 tttaccggga aatgctatac tcacaacttt cttactttat ttattagtct tatttattca
     7501 aattattggt tcaaaattat atacagaatt aataatgact aatttacagc tagtttttaa
     7561 tttaatgttt gtaattcaag gtatagcagt ttgcatttat ttcttaaaga aatggataag
     7621 acagggacca aataaaatta tgtttttttc agggataatt ttatgtttat ttggatttat
     7681 gggaatatct tttgttggta tggtagatag tataattgac tttagaaagg taagaagcta
     7741 taaatccact taggaggata gaaatgagta ataaacaaac ctttaaatta aatatgccag
     7801 aaataaattt atatataatt gttattggta tttctagtat aattttgctt tattacaact
     7861 tgtatgtagg gtgtttattt ttctgtattt ttgtatatat ggtttttcac aattggagaa
     7921 ctactaatat tagacgtcat gaatggacag agtatattca aaatttatct ttagatatag
     7981 atgaaacaac taaaaaagcc ataataaatc ttccaatacc attatgtatt ttagaatttg
     8041 atggaaatat atcctggtat aatggaaagt tttatgatat gattggtcaa aaagatttac
     8101 ttgataaaaa tatagaggat atagttaaaa atcttaattt aagaaaagta ttaaatgaaa
     8161 ataaagagat gtacacagaa ataaactata aagaaaaaga atatacaata atttataatg
     8221 ttataaaaaa tgatcaagaa aaaaatccta aatatttgat gatattatac tggatagaca
     8281 agactgaata tttaaaagta aaacaaaatt atgatgatga aaaaaatgca atgatgctaa
     8341 tacaagtaga tggatatgat gaagtattaa aaagtgcagc agaagataag agagctttaa
     8401 ttaatgttga agtagagaaa atactttctg ctttagaatt aaattcaaat ggtgcattga
     8461 gaagaacttc aaaagacaaa ttcttcttgg taatgcataa aaaagagcta aaaaaattgg
     8521 aagctgagaa gttttctatt ttagatacaa taagacatat tgattatgga aataatcttc
     8581 ctgttactat aagtatagga attggtatag atggagatac attaaatgag aacctaaaac
     8641 ttgctacggg tgctctcgat ttagcacttg gtagaggtgg tgaccaggct gttgtaaaaa
     8701 caaaagataa atttgtgttt tatggaggaa aatctaaagc tgtagaaaag aaaaccaaag
     8761 taaaatccag attaataggt catgctttaa gagaggttat acaacaaagt gaccaggttt
     8821 atataatggg acataaatat cctgatatgg atgctatggg agcagcagtt ggtgtttatg
     8881 atatatgtaa atcctgtaac aaaactgcac acatagtttt acaatctgta aatgaatcta
     8941 tagaaatatt tataaataaa ataaatgaaa ataattacta taaaaagttg tttattggaa
     9001 aagaggaagc tattgacaac tgcactaaaa atacattagt tgtagtagtt gatactcata
     9061 gaccaaatta tactgaatgt gaagaactgt taaagctatc agagaaggta gtagttatag
     9121 accatcatag aagaggtgta gagtttataa atgatgcagt gcttttattc catgagatat
     9181 atgtatcctc aacgtgtgaa atggttacag agttagttca gtatatggat gaagatgtaa
     9241 caataaataa acttacagct gaagggcttt tagcaggtat aagtctagat actaaaaact
     9301 ttgcattcaa aacaggtgtt agaacttttg aagctgcttc ttaccttaga aaagttggag
     9361 cagatactat agaggtaaag aaattcttca attctgatgt aaaagatttc ataataaaag
     9421 ctgaaataat tcaaagtact aaaataatta ataatagaat atgtttagca tattcaagta
     9481 ctgagataga tagtataaat gttataatag ctcaaactgc tgatgagtta ttaaatatca
     9541 aagaagtaga agcttccttt gtattaggtg aaaaagatga tactatattt ataagtgcga
     9601 ggtctttagg acaaataaat gtacatgtac ttatggaaaa attaggaggc gggggacaca
     9661 tagatatagc tggagcacag ttaaaaaatg tttctctaaa agaagcttat aagatggtaa
     9721 ataaaataat agaggagtat ttggaggagg aagaatagat gaaagttata ttattaaaag
     9781 acgtaaaagg aactggtaaa aagggagaaa tgaaagaagt aagtgatgga tatgcaagaa
     9841 atttcttatt tcctaaaaag atggctgtgc aagcagatag tgtagcaata aaagaattaa
     9901 atgaaaagaa taaatctaaa gaaataaaag cacaaaaaga gtatgaagaa gcagttttat
     9961 taggtaagca aatggaagaa attaatatag aaatatattc aaaagcagga gaaggtggaa
    10021 gattatttgg ttcaataact tctaaagaaa ttgctgagca attaaagaaa caaaaagaca
    10081 tagatgtaga taaaagaaaa atattattag atgaaccaat aagatcttta ggttcaactt
    10141 ttgtagaaat aaaaatacat caaaaagtaa ctactaaaat aagagtggat gtaaaagaaa
    10201 aacagtaata actagaggtg attaagatgg aagatatgac gagaattcct cctcatagtg
    10261 tagaatcaga gcaatcaata ttgggttcta tacttctaga taaagatgct ataataacgg
    10321 ttacagaaac tataaagcct gatgatttct ataaagaggc tcacaaaata atatatgaat
    10381 gtatgataac tttaagtaat aaaggtgagc ctatagactt gataacatta acagaagaac
    10441 taagaaaaca agggcatctt aatgatattg gaggaatcag ttatattaca agtctttcta
    10501 ctatagtacc gacgacttca aatgtaaaat actatgctga tatagtaaaa gaaaagtctg
    10561 tacttagaaa gcttataaaa gcttctaatg aaataataaa tctaggatat agtggagcaa
    10621 ctaagattga agatgtttta gaacaagctg aaaagagtat atttgatata tcacaggaga
    10681 aaactagtga tgattttaaa tctataaact tagttttgat ggatgcctat gatatgatag
    10741 aaaaattata cactaacaag agtgatgtca ctggtataac tactggtttt aaagatttaa
    10801 acaaaaaaat aaatggtctt caaaggactg atttaatatt aattgcagct agaccagcaa
    10861 tgggtaaaac tgctttttct ttaaacttgg ttcaaaatgc agcattaaaa ggtgatgcct
    10921 ctgtagctgt atttagttta gagatgtcta aggaacaatt agtacaacgt atgttatctt
    10981 cacaatctag tgtagaatta aaaaaaataa agactggaac acttaatgat aatgattggc
    11041 ctagaattat agatgctatg gcagtattat cagatgccaa aatacacata gatgatactc
    11101 ctggtataaa aatatcagaa ctaaggtcaa agtgtagaaa gttaaaaata gaaaaaggac
    11161 tagatttagt gcttattgat tatcttcaac ttatggaagg tgaaggtaat aatgaaagca
    11221 gacaacaaga gatatctaag atatcaaggt cactaaaaat attagcaaaa gaattaaact
    11281 gtcctgtagt agcactatct cagttatcac gtgcaccaga acaaagagca gaccatagac
    11341 ctatgttatc tgatttaaga gagtctggag caatagaaca ggatgcagat atagttatgt
    11401 tcttatacag ggacgaatat tatcatgctg attctgagag taaaaatata ggagaagtaa
    11461 taatagcaaa aaatagacac ggtgaaactg gttctgtgga acttgtttgg cttggtgaag
    11521 ttcaaagatt tggggataag ttgagagacc tataatctaa cgaatatcca cagaacgtcc
    11581 tttctttcta caggaaaaat gtggatacat gtcactaaat atgtccaaac attgaaattt
    11641 agctaaaaaa ctatccacac tatccacaga tgacctgttg ataagtgtga ataagtagat
    11701 aaagcaagct tttggcttgc tttatctgtg tataaatatt aattattagg aggaataata
    11761 tggaaaaaat aagcttaaaa tatatatatc ccaatataat taaagtactt gatgaaataa
    11821 acttatttag agtaattgac aataatttaa gagaatctat tgttgtttat gctaataatg
    11881 tggataatca gtatcatata aacatgacta atacaaactt tggaaacata attaacatat
    11941 gtaaattaga aaaactttta gatgtggata aatttatgga aaaagttata aaatatgaaa
    12001 aagaaataat agagaaagaa gagttttcaa aaatagaaga atatatgtta aatattggag
    12061 aatactaaaa aagttgaaaa aaatacgaat gtttgataga ataagtaagt aaagtgttcg
    12121 agagtatata aaatatagag gtgaattaca tgaaaacagt tgcaattgtt ggttctcaat
    12181 ggggagatga aggtaaaggt aaagttatag attatcttgc tactcaagca gatgtagtag
    12241 taagagggca aggtggaaat aatgcaggac atacattagt tgtagaaggt aaaaaatacg
    12301 cattacatct aattccatct ggagttttaa atccaaatac agtgaatata atcggaaatg
    12361 gaatagtgtt tgaccctaaa ggatttttag aagaattgga aatgtttaaa acggataata
    12421 taagtacaga aaatataaaa ataagtgata gagcacatgt catattccca tatcataaag
    12481 aactagatgc attgtcagaa gaagccagag gcgatttaaa gataggtaca acaaaaaaag
    12541 gtattggacc ttgttatatg gataaaacag agagatctgg tattagaata tgtgacttaa
    12601 tggataaaga taaatttgct ataaaattaa aagctcaaat agatgctaaa aatgaaatag
    12661 ttaaaaatat atatggaaaa gaagaattat ttgattttga aactatatat aacgaatatc
    12721 taggatatgc agaacaaata agaaaatatg tggcagatac gtctgttatt gtatatgatg
    12781 cagtaagagc aggtaaaaaa gtattgtttg aaggagcaca agggacttta ttagatttag
    12841 atttaggaac ttatccattt gttacatctt ctcatccaac atctggtgga tttgcaattg
    12901 gtgcaggtat aggaccaaat atgataaaag acgttgttgg tatagtaaaa gcatatacta
    12961 caagagttgg agaaggaccg tttgttacag agcaaataaa tgaaactgga gataagataa
    13021 gagaacaagg acatgaattt ggggtaacta ctggtagacc aagaagatgt gggtggtttg
    13081 atgcagttat agttaagtat gcagctagag ttaatggatt gactagtatt tcatttatgt
    13141 tacttgatgt attaactgga tttgataaga taaaagtttg tacgtcttat aaaatgggcg
    13201 ataagataat aacggatttc ccagcttcat tagatgattt agcaaaatgt gaacctgtat
    13261 atgaagaatt agatggatgg aatgaagata taactcaaat agataacttt gatgaccttc
    13321 cagaaaatgc aaagaaatat gtagcaaaaa tagaagaact agttggagta agtgtagata
    13381 tggtttctgt tggtccaaat agagctcaaa ctataataag aagaaatata tttgcttaat
    13441 attttatcaa ttaaataatt taaaatagtt gaagaagagt atctcaagat aatgtttata
    13501 gttattagag atgctctttt atatttgact tacattgatt ttttattaag ttaaagctac
    13561 ttatacatat tctagctgaa attaaagttg aatttatgta gatacatata ggttttttaa
    13621 aaagaatttt gttgaaaata aatggctaaa gatgatttta attatattct aaaaaaagta
    13681 aaaaaaacta ttgactaaaa attatataga cgctataata taacttgtga gtgaaagaaa
    13741 cgacgaaaaa agtcgaaaaa atgtggtcta ttagctcagt cggtagagca cctgactttt
    13801 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaatatggct cgttggtcaa
    13861 gaggttaaga caccgccctt tcacggcggt aacaggggtt caattcccct acgagtcacc
    13921 agtatgggac tatagctcag ctgggagagc acctgcctta caagcagggg gtcacaggtt
    13981 cgagccctgt tagtcccacc aaattttgaa ttagagataa atactagatt atatgctggt
    14041 gtggctcaac ggtagagcag ctgacttgta atcagcaggt tgtaggttcg attcctatca
    14101 ccagctccac aaagtacata aattcaaatt aatataattt tctatgttac aggatacttt
    14161 taagtatctt tttttaattt catttttatt gtattaaaaa tgaaattaaa catacaacaa
    14221 aattttgaat tacttgcaat gtgatagttt taaatataaa atttagaatc attctagcat
    14281 agtattatgt aatatagcaa atttaattta tagtatcaat aaatatagac tactaagaat
    14341 gtaaatgatt tgttagatta agatacatta gatatgtaaa tagaaaaata aaaagtatgt
    14401 tttaatttat gtgatataag ttttaaacag agaagcaagt ttgctcttac ctctctattt
    14461 aaaaataaaa aaatgttttt taagggctat tttatttaaa tctttcccat ctcaaatcat
    14521 ctccagtaaa ttctaaaatc ctaaacttat ctaatattct agagagagtt ctttgagtgt
    14581 aagtatttcc tatttgagat ggtgttaagt tggttgaaat aatgattttt ttaccagcaa
    14641 caagtcttgt atttacaata ttgaagattt ctccactagt aaaagaatta ttaagttcag
    14701 ttccaaggtc atctattatt aataaatcac aatcaaacaa gtttttataa ttatcttcac
    14761 ttatttgatt atttgtgtct cttctaaatt tataatcttc aagaatatct aaaattctaa
    14821 aagaagtttg atagattaca acattgcctt tatctaacaa ttcctttgca atacaattgc
    14881 acatataggt ctttcctagt ccagtagaac cataaaacag tagattttca ctattatcct
    14941 tcttaaaatc atgaacaaaa ttttcgcaga tacttaaatt atttagcata ttttctctag
    15001 gagatatttc accatcagag cctttcttag gagaaaatat attcaagtta aaattcgaaa
    15061 aattttcctg acttaaaatc ctatctaaat tagacatttt ataagcttca tttacaattt
    15121 cttgtttgag gcaagaacat ttttctccat taggtaaaaa tcctttatct ttacaagaaa
    15181 tacattgaaa ctttatttcc aagtaatcaa gaggtatgtt actttcagct aaaagtcttt
    15241 ctttctttac ttttaaagac tctatattct ctttagtctt ctttacaatt tcatctttag
    15301 acttgggatt taaaagtact atcttagcta aactgagacc tattttagaa atttcatcat
    15361 ctatagattt tatctcaggg attctattat atacctcatt ttttctatgt tctaataaga
    15421 gttcattatc atctcttctc ttagcatatt tagcaagtat ttttcttatt ttatcctcat
    15481 tcatttaatc acctacttaa acttttcttt ttgacttttt ttaataatct catcgagctc
    15541 atcagaggta tactgagtga atgtttcgtt gaaattatga aacttagttt ttttgtaagt
    15601 attgttgttt tgagtagtat taatctgctt cttattaata ttctccttaa ctatacgttc
    15661 ttcctctttt tgctttaaat catttaaatt ttttatattt ttttcattcc aatttttaat
    15721 tataccattt atataagata tacttgggtt tgaaatattc ttagacttag aacatgcatt
    15781 tattatcaaa tctatatcca tattaaattt atcaaaccag gtgtccataa gttctttttc
    15841 tgattttgat ggttgtcttg aaaatcccaa ttcattgaaa atcgttttat ataaaatata
    15901 tcgttcactt cttaccaaaa agctttcttc aacatctttt ggcgtatata aatttgaatc
    15961 ataccaattt ctaataatgc cttcaatgta tttaacgggc tttgatgtac cagttttatc
    16021 tttgacgtat tcatatgcgc aaagtatcat atcggggctc atattgtatt tattcattat
    16081 atccataata ctcatctttt cgcttgggtc gagatatcgc ccaactattt tatttataga
    16141 attaaacatt tttctaatac ttggattttc agaagtagat acaaccttat ctgtattaga
    16201 ttttatagat gaattattgc ttaatatatt ctcaatatag aaatttttta aatctaaaaa
    16261 ttctattgaa taattaaaat tatcatattc tccattgtca tgtattttta taatcttctt
    16321 ttcttcccag aatttccaag cagataacac atcagacaat ggtatattta ggtttttggc
    16381 tattgaatta ttgtcgaact taggattaga agtaatatca catgcttgtc tatatcctaa
    16441 aagataaact tttacgtata acccgtccgc cattggcata aatatatcta taaagatatt
    16501 gggaatagtg atttcaccta aatctatttc attgctttct ttaaaaaaca ttttatcacc
    16561 tcagattaaa ttatatcatt ttttatatta tatttgtctg taaacaaata aaaggtcaca
    16621 agattagcat gtaaatattt tctaaaagta ttcattttga aaaggaggag atattttggt
    16681 tataaaattc aaccaaaaag taagaagaac attatcagta gcgattctat ttatggtatt
    16741 aataataggt tgctttatag aaaaggatga cagtttagaa acatacaaag aaaaaactaa
    16801 ttcaaaaata aatcagtata atatggatgt tatatttgat gatgagacta agaggttaat
    16861 gtgcaatcag aatgttgact acataaataa tacaaaatca aacatagata aaatatattt
    16921 ccatatatat ccgaatgctt tttccaagaa agattttgct ccttttgaaa aaagtgaaat
    16981 gacaagggct tatcctaatg gatttaacga aggatatata gatataaaga atatattaaa
    17041 taataatagt aaaatggagt ataaaattaa aggagacaaa aatgacatat tagaaataga
    17101 attagggaaa gagttaaaac caaatgaaag aatgtcatta gatataaaat ataacgtaaa
    17161 aataccaaat tcaattggaa gatttggata tggagaaaat actataaatg ttactaattg
    17221 gtttcctata gcttgtgtaa atgatgaaag gggatggaat ttaaagagtt atgaaactat
    17281 tggcgaccca ttttatagtg agactagcga ttttcatgtg aaactattga ttccaaataa
    17341 atataaaatt gctcatacag gaaaacttat tgatgataaa catgataata agaaaatgct
    17401 atatgaaata gaagctgaaa acgttagaga ttttgcattt atactaagta gcaaatttga
    17461 tgtaaacaaa gtagattcaa agggagtagc tatcaataca tataatttaa ataaaaagct
    17521 ttctaaaaaa gcaactgaag tggcaaaaga ttcaataaat atatttagtg aattatttgg
    17581 aaagtatcct tacaaagaat tttctgttgt agcaagtgac ttttttatag gggggatgga
    17641 ataccctatg cttgttatga tagaccaaag tttatataat gaaaaaaatg aatttttact
    17701 agaatatgtt attgcccatg agacagcaca tcaatggtgg tattctgctg ttggaaatga
    17761 tgaaataagt gaaccctggt tagatgaagc acttactgaa tattctacaa tagtttattt
    17821 tgaacaaaag tatggtaaag atatggcaaa caagcttata aaaactatgg agatacagac
    17881 aaaaagttat ttgagcgaaa atatatttaa gccagcaaat gagtataaaa attctactga
    17941 atacagcttg aatgtttata caaaaggcgc aatagcattt aatgaaataa gaaaagaagt
    18001 tggagataaa gtattctttg agactttaaa tgagtactat aataagtata agcataaaaa
    18061 tgctaatgga agagcttttg tggaactatg gaatagtaaa ggtgtagata taaataagat
    18121 tataagtgaa tgtaagtaaa tagtgaatgt aagtaaattt tgtgcagagt attaaatttt
    18181 atttaatact ctgttttttt ggtataatat aatttataag gcagaaaaag aggtgaagga
    18241 atgctaaagt attgttcagt agggagtggt agtagtggta attgccacta tatagggtat
    18301 aaaaatacta atatactagt agatgcagga ttaagtggca agagaataac tacaggtctt
    18361 aaagacattg gtgtagatgc agataaatta aaaggaatat ttataacaca tgagcatgtt
    18421 gaccatataa aaggagcagg tatattatca agaaagtttg atataccagt atttgctaat
    18481 ataaaaacat ggtgttctat gaaggacaaa ttaggtgaca tcaaagataa aaatatgaaa
    18541 gtatttgaaa atgataaaac ctactcatta ggtgatatca tagtaagacc tttttcaata
    18601 gaacatgatg cgtctgaccc agttggatat aatttttata cagagagtga tgaaaaaatg
    18661 tctatagcta ctgatatagg atgtataact caaaatataa aaaaacatct atataaatct
    18721 aaattagtag tattggaatc aaattatgac cctaatatgc ttatgatggg ttcatataca
    18781 tatgctttaa agaaaagagt aatgtcaagt actggtcatt tatccaatga agatgcagct
    18841 aatttctgcg ttgaacttat taatgaaggt acagaatcta tattattagc acatttaagt
    18901 aaagaaaata attttcctga gttagcgtat gagacttcaa aaggagtatt agcatcaaat
    18961 gatatagtag ttggacaaga tgtaaaacta gatgtactat cacgaaatga tgtttctaat
    19021 gtttatgaga tgaagtagag gtgaataact ttgctaaaga gaaattttga aaatgttgag
    19081 gataataaaa ttttatactc tataaagcag ggtatgattt tagcgatacc tgctattatg
    19141 actggttcaa ctgctcttgt aattttgaat cttccaataa aggcttatca agaatattta
    19201 tcaggcttgt ttaatggaga ggttacaaat attcttaact ttataaatga ttctacttta
    19261 ggaattattt cattaataat attattaaca ataagctata gttatggaaa aatatatggg
    19321 agcaagtata cagtacttgt tcctatagtt gcaatgtgct cttttttagt attttcacat
    19381 ggtaatgagc ttaattctta tatagaaata tttaaaacaa agtggttatt tacatctata
    19441 attgtttcta tgacatcttc tgtattgttt gtaaaactta cagaatcttt tgttacaagt
    19501 gtaaagtttc atacagaagg ggctgatgca gactttaata tggtagtttc ggctatagtt
    19561 cctttcataa tagtagtatt tttgttttct atttttagag tgataatgat aagtctaatt
    19621 ggcagttcaa actttcaaga tatattttat aatttatttt caaatgtttt taataaaatg
    19681 ggaacaaacc ttatgagtgc tctgttgttt atttttttaa tgcactttat gtggtttttt
    19741 gggattcatg gtagtaatgt attagatacc gtcgcaaaaa atttatttga aaatagtatg
    19801 gcaattaaca taaatttagt taataataat caattaccaa cagaaatttt tactaagacc
    19861 ttttttgata caatggtttt attgggagga tgtggttctt tactatgttt agtaattgca
    19921 atttttctta gtgaaaaacg tataaatgta agaaagttag ctaaaattgc ttcaatacca
    19981 gctcttttta atatcaatga gatgctagtt ttcgggatac caattgtttt taactatatt
    20041 atgtttgtac cttttgtaat tactcctata atcttaacca ttacaagtta tgtggctatg
    20101 tctacaggta ttgtaccatg tacagtttca gctgtagaat ggacctcacc tatattttta
    20161 agtggatata tgtctacagg ttcaataaga ggtagtttgt tgcaagtatt taatttatgt
    20221 gttggtgtta tgatttatat tccgtttata aaaatgtcgc aaaatagata tacatatatg
    20281 tttaaaaata atatagaaaa tttaacaaac ttattaaaaa ggtatgaatt aacaggtgaa
    20341 cagcctaatc ttctaaacca taatggtaaa ataggttcaa tttcaaaaat gttggcatca
    20401 gacttgaagt ttgctatgaa aagaggagaa atagagcttt tttatcaacc acaagtcaat
    20461 tataatggaa atgttgtggg agcagaggga ttacttagat ggaaacattc aataggaggg
    20521 ttcatttatc caccactagt tataattttg gctaaagaag aagggttctt agatgagctt
    20581 ggagagtata ttattaataa agcctgtatt gatttaaaga agagtgaaaa gctattgaga
    20641 aatcctgtta agttctctgt aaatatatct ccagaccaac ttgatgaccc taagttacca
    20701 gagcaaataa aaaatatcat tagtagtaat gatataaatc ctaatatgtt aggtatagaa
    20761 ataacagagc aaattgcatt atcaggttca caaataatta ttgaacgtat caatgcgata
    20821 cataacttgg gaataaagtt aattatggat gattttggga tggggcatag ctctcttata
    20881 tatcttcaaa ataataactt tgatatagtt aaattggatg gttcattagt taaagagata
    20941 cttacaaata aaagaagtag tgaaattata atgactattg taaatctttc aaaaaattta
    21001 gattttgata tagttgttga gtatgttgaa aatatagacc aaagggataa gttatatggt
    21061 ttaggatgtg aaatatatca aggatattat tatagtaaag caataccttt tgaagagttt
    21121 gtagaatatg caaatcaaga aagagagata taattttata agagcttgta aattctagta
    21181 atgcatagat ttgtaataaa attaagttta ggagaaatat atgaatataa gtatagtcgt
    21241 agtaggtaaa attaaagaaa aatatttaaa attaggtatt gatgagttca agaaaaggct
    21301 ttctaagtat tgtaagttag aaattataga gttggatgat gaaaaagctc cagagaattt
    21361 gagtgttaag gaaatggaaa ttataaaaga caaagaagga aagaaaattt tagggaagat
    21421 aaagcataat agttatgtta tagccttggc tattgatggg aagggtttat cctctgaaga
    21481 acttgctaag actatgagtg atttagcagt tagaggtaat agtagtctct gttttataat
    21541 aggaggttct ctagggcttt ctgatgaggt tttaggaagg gctgattata ggttatcttt
    21601 ttctaggatg acttttcctc atcagatgat gagattgata ttgttagaac agatttatag
    21661 ggcttatagg ataaataatg gagaaccgta tcataaataa atgatacggt tttttatgat
    21721 ataattgaat tataaaataa aatcaaaatg taaagtaggt gtagtaatgg acagactaga
    21781 tatatttatc gaaaattatg attttaaaga atatcttgaa aaattaccta gagaattaat
    21841 agaagaaaat tataaagatt ttgttataga attttggaaa aatcctagtg tagatattat
    21901 taaactaaaa tcaaaatata gtataaaaaa tgtagtatct ataagtagta tattaatgca
    21961 aaatcctatc caagagtatt atgagtttct tgaagatatt tggaaaaata acataaaaat
    22021 agatgaatta attaataagt atttaattac agcaacaaga atggatcaaa ttaaattccc
    22081 attaatatat attgatgaag attctttttg tccttattgt ttaggtaata aattcaatat
    22141 tattaataat attaagcaac aagatataaa agttgaatgt gaaaagtgtg gtgaggcaat
    22201 tagttatttt attcataagg aagaagtaga taatataata gaagaagaaa aaagaaaaaa
    22261 agaactattt ataagaacat tggagaatgt atataataaa ataaacaaga ttaaatgtca
    22321 tagatgccaa tcagatttat atgtgtgtat taatgataaa ataatggagt ataatattag
    22381 gtgtgataga tgtaattcac agtatgaatc ggttgaaatt gcacaggaac aatatagatt
    22441 atggcaacaa agatctgcaa tgatggctaa cataaaaaat aaagaaaatg aattgttgct
    22501 aaagacacta tcaattaagg atgaaaaaga tattaaattt aaactcgaag atataattac
    22561 ggagagtgaa agtatagatg taattagtga tttagtagat atgcaagata gaggagagaa
    22621 tattttcaag caattattta ataattatgt tagactagga agattagaca gaaggttatt
    22681 ggtaactgtg tgtaaagatt gcaagcaaaa tgaaaatact tatacatggg aaagaggcaa
    22741 agaacattta ataaatatga gggggattac atacagtgaa gcagttgtat atgagttaat
    22801 ggaaaaaaca aagctattgc caataagagc aattattaga agacttatgg agaaaaaact
    22861 attgtattgt gatgaaagga gcaataattt acaagtacac cctgttttga ttgaaaattt
    22921 agctaaacta gaatcaataa ataaatctca aaatataaat aatcaagtag cttatctaat
    22981 attcaataag caaaaatata tatgctatca ttgtggtgaa tcaggaagac ccttaaagat
    23041 aggatatcta acaatagata aaaatataaa tgatttatca agtttagtgg gaatatgtga
    23101 tagttgctat gaagatatta ctgaagatga aattattata gataatattt tttgtctggt
    23161 agatgaaaga aacaatgaat taggggaagc ttggaaattt ttaatacaat atgatagtag
    23221 ttttagaaat aatacaattg cagaagtaga gcatgcaaga ttatatgata tgtatgatga
    23281 agataattta attcgtgcgt atgcaaatac attgtataaa caaacgaaag atgggtttgc
    23341 taatgaaaaa gtattttttg catatgcaag agcaatattg aaaaatagca aagggcaagt
    23401 aagtgtacta caaatagcaa aagaaaaatt taaattagaa aagtggttaa atggataaag
    23461 tgataataca tattaaaatt ataaatttat ataaaactgt attgcaatta agcaatatgg
    23521 tttttatata taagataatg atacaattaa ccaaaatgtg gtagtacata agtgttatga
    23581 gatttaaata ttagcgatta aaataatgat taagtaaaat tgtaattgat gaggagtatt
    23641 aacgtattag tttattttta tatataatta aaatatagga gggaagaatg tgagtcatag
    23701 taaactatct gatcacaaat taaaaaaggg gaaatttata gcacctttta atgaatttat
    23761 gacaccattg aatagtaatg agtcatggtg ccttggtcga ttgccagagt atatatggat
    23821 aggattatta ataaataaat atggtagaaa aaaagggatg gaaataattt ataaaataat
    23881 tgtaaaactt cattcaattg aatcagatat tgtaacacct aggttgtctg agattttatc
    23941 tatggaggat gtaaatcagg atgaattctt tagttatatt ctaactattg ttgaaaagga
    24001 agctttatct ccattgacat taatatttac atatagtaat tatcctattt ttgttaaata
    24061 ttttgcagag ccaagtataa aaattgaaga tagggttacc tatttagatg agactttaag
    24121 aaaagctatg catcatcaaa gtgaatttgc tacagacata agatttatag ttttatatta
    24181 tttttgtatg tcaggaaaac tcgtattgca aaaagaacag attaataatt tgctggaata
    24241 tcctaattta gaacacactg atgaaaaaat gagaatgtta cgtccgttta ttcgtgcagc
    24301 agaattaatg gttttaaata tggaaaaaaa atctgattgg tatttagata ttttttgggg
    24361 gaggattagt gttatgactg aatgtaaatt atacgcaact aaatttgaag atgaaactgc
    24421 taatacagac ttgtatatgg aacaattaca tgaggttttt gtttatttat cagatttact
    24481 atgtaaagta aatccattag acgagaaaat gttagtatta cttggaatag caatatattc
    24541 ttataaacga ttacgagaag tagttgagca taaattattt aattccattt caggtagagg
    24601 ctcagtaagg gtattgatag aaaattatat tatgatgaag tatttagtaa aaatagaagc
    24661 tcaacatgaa aatatatggt cagaatatca attttatgga ataggtcaat ataaattaat
    24721 tgtggctaga tatagagaac aagaacaaga tttaaaaaat agtcatgttg attataaata
    24781 tattgaagct ttagttaatg aatttgtaat tgaagaaaca atagatatgg atactaaata
    24841 ttttgataag caaaatatac gtagtaaggc tgaaatagtt ggagaaaaag agttatatgg
    24901 gttgtactat gattatgatt ccgcatatga acatggttta tggggatcaa taagagaaag
    24961 ttcaatgtta aagtgtgata atccagctca tcactatcat tgtataccag atgtagacaa
    25021 taatcaaaat ttaaaaagtg tttggcatga ttgtgtaaac ataatggaaa aaactttact
    25081 tttcttagat agtgtatatg gtataccaca acatttgcta ttagaggtga taaaaaatgg
    25141 aaaagaattt tttactaaat aaaataaata gtattcagaa ggaatattta gaattattag
    25201 taaagttaaa agataatata gatactatag attatattta tataatagat gaaataaata
    25261 tattttggta ttcatataga gatgttataa agctttactt agggaatatt acacaaaaag
    25321 acaatgcata tatttttaca ggtgcaacat atctagatgt tgatgattta gaacattatc
    25381 catttgttat gatgggagat atacatatag tagatgattc gctatgtaaa tattcaaaaa
    25441 cagtactagt attatcaaat caggaatttg ctaaagtatt aaaagaccaa attatttctt
    25501 gtatagatga taatatcaaa atattgtcta attattcttt tgcgatgtta atattaccaa
    25561 tgaatttact atatggtaat gatatgaaaa taatacatga atattcaaat aatatatttc
    25621 tgaacttttt ttcaagaaaa gacttaaact tagatactta ctttgaagaa tttaactcaa
    25681 tcgaagatat agctctagca ttgaatccag gtatagagag ttatattgtt tttgacgaag
    25741 gtgaagattt aactaaaaat ctggaagaaa gatttaaaag ctttattgat aaggggaagg
    25801 tacccctacc agcacataaa agtgatgctt ttaaattttg gctgataatt tatggattta
    25861 taactcaggc attagatatt atattgtcat gtaaaggatt taacctaact ccttatttaa
    25921 gatatagagt tacattacaa tatgttttat tactaggcga taatgttttt gatattgaag
    25981 atctcagcaa tatgttgtta aaatgtaaag tagctcatgt actttatgca atatttgata
    26041 aaagcgaaat aaaaaatatt tcctttgttc aattttataa gaaattaagt gaatataaat
    26101 ttagctataa agtagaagat gatatcaaag aaaatataaa tataaatcaa attgctgaaa
    26161 ttttaaataa aaatattaca ttgtttttaa attatatata aatttattta tataattaca
    26221 ttccttaggg tacataacag aaaaaaggct gtcgcactaa aaaatagtgc tacgcccttt
    26281 tttatacaaa aaaataaagc tcaagttcaa aagagcctgg gattcctgta taataattgt
    26341 atgctaaaac aaaatactat acaaattgat tatactactt atggtaataa ttatcaattt
    26401 aaattaccac taaatattga ttatatgatt ccaaaagatg ctatatttat tgatggtaca
    26461 aaaatagagt catctgccaa tagatatact tttgtttgga aaaaaactat aaacaaaaat
    26521 atggttagag tgatgaatga tatatctaat ttcatattaa aatgtgagat tgattttggt
    26581 attaaaataa tttatagaaa taaaatcaaa atatatcatt taaaaaaact tttaaagaag
    26641 cttaaaaaaa tatcaaaaga atcaaatata acatttgttc atggaaaagg aaagaaaaaa
    26701 tctatagcga aattaaataa ttatatcgat agattgaaaa aatatactaa agaccttcat
    26761 ataatgggtg aacgaaatag ttattctaaa acagataatg acgcaacttt catgagaatg
    26821 aagaaagacc atatgaaaaa tgggcaattg aaaccagcat ataatataca atttggcgta
    26881 gattcagaat acatagtttg ggtaagttca gagcataaac ctactgatac tacaacatca
    26941 ataccatttt taaaatctat tgaagagcat acaaaattta aatatcctaa aatagtagct
    27001 gattctgggt atgaaagtga ggaaaactat acatttttag agtgtaataa tcaattatct
    27061 tttataaagc ctgcaaatta tgaaattagt aaaactagaa aatataaaaa tgatattggg
    27121 cgtattgata atatggatta tgatgaaaaa aatgattatt atatttgtaa aaatggcaaa
    27181 aaactatttg ctaacaaaat aatcaataga aaatcaaaaa caggatataa aagtaaaata
    27241 acttgttata cttgtaaaga atgctgtgat tgtcaatata aaaacggttg tattaaggga
    27301 aataatagta agattcctct tgaaaataga actaaaaatt tacaagtatc taagttattt
    27361 catgaaaaaa gaaaagaaaa tttaaagaga attataagcc aagaaggttg tcaacttaga
    27421 atgaatagaa ttatacaagc agaaggaact ttcgctcaag taaaacaaaa tatgaaattt
    27481 agaagatttt taagctgagg caaagaaaat gttatatcag aatgtattat tgtagcgata
    27541 gctcataaca tttctagact acacaacaaa ataataaaaa ataaatgtgg aatatatctt
    27601 catcatttaa aagaagtatt ctaaatttat tatttatagt tagatattat ctgaaaaaat
    27661 ggtattttta tacctttatt tgttatgcat aattttactt agaacataag ctttatataa
    27721 aacaattaaa aatatatcta attaagataa gtaaaaaaag ctgtcacaga acaaatttaa
    27781 tttgctaatg cgacagccca tttttgttaa tttgacactt aataatatta aaataacaaa
    27841 aaagtattga aagtatattg catttaaggt agaattaaat aaaatattat aacattataa
    27901 tgtttatata gttgttaata aaaatttgga ggaaaatata tgataaaaat aatgttggcc
    27961 tgttcagcag gaatgtctac aagtttatta gttactaaaa tggaaagtgc agctaaagaa
    28021 aatggtatag agtcgcaaat ttgggcaata ccagaatcaa ctatacaaaa tgaaatagaa
    28081 aaatgtgatg tattattact aggaccacaa gttagatatg tattacctaa ggctcaagaa
    28141 attgctaagc catataatat accagtagaa gttataaata tgatgcacta tggaacagtt
    28201 aatggagaag ctgttttaaa tagagcgata gaacttaaca acactaagta atataaatta
    28261 taatgtattt ttaatatata aattgggaga atcaaaggat ggatgaaaag attatagaaa
    28321 tatcttttaa tataataggt tatgcaggtg aaggtaaagg gttagctttt gaagcaataa
    28381 aagaggctaa gaatggaaat atagaaaaag caaaagaatt attaaaagag tctagggaag
    28441 ttataaataa agcacataga tatcaaacag aattaataca aaacgaagct agtggaaaca
    28501 ggactgaaat aagtgtcata cttatacatg cacaggacca cttaatgaat ggcatgaatt
    28561 ttcaacagtt agctgaggaa atcgttgatt tacatttgaa attacaagat aaataaaaat
    28621 taaagataat aaattaagag agttcttaaa agatacatta tgtgtagcac actaattgtt
    28681 agattattga ttcaatattt agtgagtaat acattaggat tattttgagg actcttttta
    28741 tatttagttt aaagttaata aatatttatt attaataata taa
//
LOCUS       DACXMZ010000022        28740 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.022, whole genome shotgun sequence.
ACCESSION   DACXMZ010000022 DACXMZ010000000
VERSION     DACXMZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.022"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(359..1198)
                     /locus_tag="JD016_03890"
     CDS             complement(359..1198)
                     /locus_tag="JD016_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAU4998769.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            complement(1412..3109)
                     /locus_tag="JD016_03895"
     CDS             complement(1412..3109)
                     /locus_tag="JD016_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAU4998770.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEVVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            complement(3175..3765)
                     /locus_tag="JD016_03900"
     CDS             complement(3175..3765)
                     /locus_tag="JD016_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HAU4998771.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            complement(3875..4672)
                     /locus_tag="JD016_03905"
     CDS             complement(3875..4672)
                     /locus_tag="JD016_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF348 domain-containing protein"
                     /protein_id="HAU4998772.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGIKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            5028..5549
                     /locus_tag="JD016_03910"
     CDS             5028..5549
                     /locus_tag="JD016_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU4998773.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSSLQEEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            complement(5968..7182)
                     /locus_tag="JD016_03915"
     CDS             complement(5968..7182)
                     /locus_tag="JD016_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4998774.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYNLKEIL
                     EKYPIHENLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(7647..8582)
                     /locus_tag="JD016_03920"
     CDS             complement(7647..8582)
                     /locus_tag="JD016_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009109552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HAU4998775.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            8870..9436
                     /locus_tag="JD016_03925"
     CDS             8870..9436
                     /locus_tag="JD016_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAU4998776.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRKRVNKND"
     gene            complement(9627..11663)
                     /locus_tag="JD016_03930"
     CDS             complement(9627..11663)
                     /locus_tag="JD016_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HAU4998777.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDENYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(11837..12514)
                     /locus_tag="JD016_03935"
     CDS             complement(11837..12514)
                     /locus_tag="JD016_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAU4998778.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNSVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            13925..14110
                     /locus_tag="JD016_03940"
     CDS             13925..14110
                     /locus_tag="JD016_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAU4998779.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(14152..16443)
                     /locus_tag="JD016_03945"
     CDS             complement(14152..16443)
                     /locus_tag="JD016_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HAU4998780.1"
                     /translation="MKNLSKKAVILSLSLVVVSPLVHKINAQDIEKNREVRYETRKAN
                     NLENMTMEEAFPDENFRKVICDKLSILDDGNPIGISQKAIIESTKGLTLGSKSIKNLS
                     GINYFIGLENFSCRGNNLTSLDLSSNINLKELYCNENQLTSLDLSNNRELTTLHCGEN
                     NLTDLVIENSKLDELNCRKNNLKSLDITKATNLTTLLCFENELTSLNLSKNQKLKILK
                     CDYNNLNDLDISTNLELQDLNCSFNQLENINLDNNKELVKLICHNNKLQNLNLKNHEK
                     LVKLYCNQEQLSTLNVENCINLEDLTCSRSNLKNLDISSNKNLKYLECVANKLTNLNI
                     ENNIELLELYCYDNEIKALDISKNIDLENLTCYKNKLDSLNTSKNKNLKYLYCSQNEL
                     TSLDVTKNTELVRLYCGENRLTSLDVSKNTKLKELDWSNQKKSTSTGGSSGSGGGSSS
                     TNEESSEPTSTPSKEKLTGVDRNETSVKISQKGWNKADNIVLINDSSISDALSATPFA
                     KSKDAPILLTKNNNLNKLTEKEINRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRI
                     SGNDRYETSIKLAKELDKNSNLSKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQND
                     DMKDIKDLIANKNISKSYVIGGESLFNNKEVNNTLPSVTKIAGSDRTETNSKVISHFY
                     SKDTLNDLYVAKNGMNKQDDLVDALSVGVLAGKTESPVVLVGNGLDNSQKELIKNKKF
                     KNITQIGGNGNEKAFNEVENLVK"
     gene            complement(16600..17412)
                     /locus_tag="JD016_03950"
     CDS             complement(16600..17412)
                     /locus_tag="JD016_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAU4998781.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            complement(17491..18354)
                     /locus_tag="JD016_03955"
     CDS             complement(17491..18354)
                     /locus_tag="JD016_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HAU4998782.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKNNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            complement(18615..18872)
                     /locus_tag="JD016_03960"
     CDS             complement(18615..18872)
                     /locus_tag="JD016_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAU4998783.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            complement(18892..19122)
                     /locus_tag="JD016_03965"
     CDS             complement(18892..19122)
                     /locus_tag="JD016_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998784.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLIGFFIFAIQRGIF"
     gene            complement(19174..19737)
                     /locus_tag="JD016_03970"
     CDS             complement(19174..19737)
                     /locus_tag="JD016_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998785.1"
                     /translation="MTFKELVNKVRNLILEAKKVTIEDTENKFTSENVEEALKECINR
                     ADEAFQEADSGKELIATAIGSPATSEQTFQEYANYITEFKGTITDLQQQVNMRYKISA
                     GSFEGEETKPYKVTFPSVPTHLVVFSIMNEKECYYTPLKQKLESNPGGSTAYIEINAD
                     RKGFKAGSTSYTTGKSPFKGYFIACYK"
     gene            complement(19771..19953)
                     /locus_tag="JD016_03975"
     CDS             complement(19771..19953)
                     /locus_tag="JD016_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998786.1"
                     /translation="MNINNVVVRILAERILNGGLNSLKNREFQLDDVTNTEYRKAIED
                     YIIKNSGVVEGAEPTI"
     gene            complement(19953..20258)
                     /locus_tag="JD016_03980"
     CDS             complement(19953..20258)
                     /locus_tag="JD016_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998787.1"
                     /translation="MTEYIEVGRRIFFDEEGEIIFYEGQSKGNVPERKNIKKIEYIDL
                     EYDYVDYDKYKIKGINIETKQPILEEIPIYMSEEEKRIQELENQILIAENEKSGGLL"
     gene            complement(20282..20827)
                     /locus_tag="JD016_03985"
     CDS             complement(20282..20827)
                     /locus_tag="JD016_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998788.1"
                     /translation="MKDELQIGKNNIAGTLGSPFVGTDKLSVTKTKIQTLKNTLAISL
                     SMKNISAQSTETLHSLIGKVAGIANIFIAQGECTQVGEKTFQLYQVDNNTPKTCSANA
                     ISFNLAFTPKFIIVYRNAYNIGLCSFVFYSSFRNIITAVPQPSNFGEMYMPNTFDCVV
                     GHTCILPVKDYKIPHFFYAIA"
     gene            complement(21250..21558)
                     /locus_tag="JD016_03990"
                     /pseudo
     CDS             complement(21250..21558)
                     /locus_tag="JD016_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728847.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21570..22181)
                     /locus_tag="JD016_03995"
     CDS             complement(21570..22181)
                     /locus_tag="JD016_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998789.1"
                     /translation="MIEKLNENASLSDLITTFENSTKELKTSKDNFTNLLGNPFLEST
                     KFTEFEGKMQNLMNTFKENLKSKGVNSGNTESLLSLINKVANIHIPTPIYTASGECII
                     DSKGTCKRYTISTNGDVYYVRINLNFYPKLVVMYNTGGASKYFTTSYFCSYVPFIAST
                     VESYTTYLLDKSDSNIQVQKGTCILPIVYSKYGSSSFYFYAIG"
     gene            complement(22395..22529)
                     /locus_tag="JD016_04000"
     CDS             complement(22395..22529)
                     /locus_tag="JD016_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAU4998790.1"
                     /translation="MWYKIIKKYYEDELWTKEQVKTAVEKNKITTIEYKNIIGEDYIV
                     "
     gene            complement(22531..22896)
                     /locus_tag="JD016_04005"
     CDS             complement(22531..22896)
                     /locus_tag="JD016_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998791.1"
                     /translation="MIKIWDKKEKINGVEAEEILKGNYDFQTSEVFLILDDYNRVTNI
                     ESVNTIKSIYKLDKSLTALETAEKYLEMHKKQEEEIKAHEELEKNSKNATIATHEEVK
                     ALREETAALTFAMMEKELI"
     gene            complement(22915..24048)
                     /locus_tag="JD016_04010"
     CDS             complement(22915..24048)
                     /locus_tag="JD016_04010"
                     /inference="COORDINATES: protein motif:HMM:PF13964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HAU4998792.1"
                     /translation="MTIKLEEDSSIKEVMDSFENIKNDLQNAKDNLASSLGSPFISTD
                     SFSTTKTKIQTFKSTLATNLIKKGVSTSSNETVQNMVSKVDDIELGNTDIPNWYKPKN
                     IIFDGFSSSLQQYPSCIAVENYIYFLCKNTLGLSLLRYDTLTNVNNSLARPSVCDDAL
                     MVYHDGYIYRIGGYNDSSAAPSCQRYNIKTNTWSYISNMITPRGGTCGNTYSDEIHVL
                     YGYTDTNYDTVTSAISEYYKISTNTWSTKTNLPASRNRRLFVCSKTDTYTFCITGTTD
                     PSSYWDTCISSYKPAYGTLTRVEGVYLGFATSINNMLYTVYRGDTYFYVGGYNQNGVV
                     FSRKYDKKNSTFPYDSLLKAVSVKDKFIYYCENSRYKCFIPEL"
     gene            complement(24240..25373)
                     /locus_tag="JD016_04015"
     CDS             complement(24240..25373)
                     /locus_tag="JD016_04015"
                     /inference="COORDINATES: protein motif:HMM:PF01344.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HAU4998793.1"
                     /translation="MATKLEENAKLREMIDTLETAKNDLQVGKNNIADVLGSPFTGND
                     KLDTTRNTLNSIRSTFVSNLNKKGVSTASNTPFKILVENVGKIEQGNMNVPIWYKPKN
                     ISITTASDSYIRNDNYAAAIGEEVFFFIKYDKNTMYLRKYNTIANSLTSLASPAYNTN
                     FLLVSYNDEIYQIGGLSGSTALSTCKKYNVKTLTWSSLPNMGANRFGAGGEAYKNQIH
                     VFYGKKNTTDSVAVNTSEYFLVDTNTWTNKGNLQLSKYAGYTCAKGSNNFVVYGLSDP
                     NNVYSVIIANYSPIAGTISGRYGTSGSFVVPIKNYLLLGNYDYYSSGKYTTIAFGENG
                     RIFERNLGDLRIPSSYDIKAVSVEDKFVYYCSNGNIICFIPEL"
     gene            complement(25924..26499)
                     /locus_tag="JD016_04020"
                     /pseudo
     CDS             complement(25924..26499)
                     /locus_tag="JD016_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087447.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(26511..27128)
                     /locus_tag="JD016_04025"
     CDS             complement(26511..27128)
                     /locus_tag="JD016_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HAU4998794.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLYDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            complement(27121..28170)
                     /locus_tag="JD016_04030"
     CDS             complement(27121..28170)
                     /locus_tag="JD016_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HAU4998795.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLSATNTIAKGVFNIDIPLDSRFNLGE
                     YNYIAIERISEKTYKMKCETAGPIFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHKVTVTGVVSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNL
                     TINSNSIVVRGEVVG"
     gene            complement(28171..28590)
                     /locus_tag="JD016_04035"
     CDS             complement(28171..28590)
                     /locus_tag="JD016_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HAU4998796.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLNIEKNRVDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            complement(28595..>28740)
                     /locus_tag="JD016_04040"
     CDS             complement(28595..>28740)
                     /locus_tag="JD016_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HAU4998797.1"
                     /translation="IEQKLSIGEIFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLIL
                     DRM"
BASE COUNT         9323 a         4737 c         3054 g        11626 t
ORIGIN      
        1 ttctaaacct aattacagta gcttattcta atatattggt aattagaaat acaggcacat
       61 ataaaaacac atgactaaat actgcattat ttgaaaatac agaaattaac tcattaggac
      121 tataattttt atttagtata ttttttgata cttaccaatt ttatctctta cttatcaata
      181 gtcatctttt taaagatgat tataaattaa aactatccca ttaaataaaa tttgtcaagt
      241 tatactaaaa ataaaaaaag cccagaaaaa ttctagactt tttaacttaa atgtcaatat
      301 ttctttatta attcaattac atttataata ctaaatactc tcttttattt taaatacttt
      361 actttaacat ttcatgtctt atctttctaa aatacaatct tgaatttacc tcatagccaa
      421 taacgataga ccaactacta aaatacatcc aggtcataag tacaattatt tcagctatac
      481 ttccatagat tacttcatag ttagcatagt aatttgtata ataagaatat aaaaatgata
      541 ctaaaaacca agcaaaagta gcaaaaatag cacctggcgc agattcttta atagttaact
      601 ttttatttgg agtgtattta tataaaagtg taaatatcac tattattgtt attataccta
      661 cagtgtatct tagaatatcc catattttta taaatatctc atccaatcct acaagattaa
      721 aaataaaata acctattttt tccccgtaaa ctaaaaatat catagataaa aaaataagga
      781 ccaaaagcat tattgtaaat aaaaatgata tagataaaat tttaaaaaaa gacctagttt
      841 ctttaacttt ataggctcta ttcattcctt taataagagc ttttactgct cttgaaaatg
      901 tccacatagt aagtacaaaa ctgactgcta aaaatttcag gtttctattg tctattgcag
      961 aaactatgat tgaagataac acagcaaaag caccttctgg catcatattt ctaaacaaag
     1021 ctatatactt atttaaatga agaatcggta tataagctat agaacttatt gtaaatatta
     1081 aaaaaggaaa tatggacaac attaagaaga aagatacttc tgctgacttt gaacttaatt
     1141 cactgtaatt ggattttgca attatatatt caatcaattt cttatctata ccaaacataa
     1201 atatcccctt ttctatcaaa ttaatttcat tatacaatat taattatact cagattcatt
     1261 ctataatata attatttaag taaaaataac acaaataaaa gctgaaaata acaattctac
     1321 taacatttcc atattctact caatgaaagt aaattataat tttcagctaa ataaatatta
     1381 ttacaaaatt atcttataaa ataataatat tttattttaa tttttcgacc atgtcatcta
     1441 tatacttttt caaaccttga atctttttat ctgcttcaat atcactagct ccttttacag
     1501 ctatgtaaaa tttaagtttt ggttctgttc cagatggtct tatagctacc caggattcat
     1561 cttccaaaaa gtattttaaa acatcagatt taggaagatt atctatacca tttttataat
     1621 cttttttatc aactacttta atactattta tataatcaat ttgattttct ctaaaataag
     1681 atattatttc ttttatcttt tctacacctt ctataccttt taaagtcaaa gatatagtct
     1741 gttctttgaa aaatccatac tttttataaa gttctattaa accatcatat aaactcatac
     1801 cttttgaata gtaaaatgct gccatttcag atattaacaa tgaagaaact acaccatctt
     1861 tatctctagc atgagtccca actaagtaac cataactttc ttcatatcca aatacataac
     1921 ttctattttt attttgttca aataatttta ttttttcacc aataaattta aaacctgtaa
     1981 gaacatttaa tacatcaaca ttatttgctt tagctatatc tgctccaaat tctgatgtaa
     2041 ctatggtttt tattattgta ggattttctt ttaatttgtt attttctttt aatccttcta
     2101 ttatataatg tgtcagcata ccaccaattt gatttcctgt aagtaaagca tactcacctg
     2161 tagtagtttt tactgctaca cctactctat cacaatctgg gtcagttgct attacaaggt
     2221 ctgctccttt ttcttttgcg agtaatatac ctctatttaa agcttttttc tcttctggat
     2281 ttggataatc aagtcctgca aaatttgggt ctggattttc ttcttctgga actacaatta
     2341 tattttcaaa accaacttct ttcaatgctc ttcttattgg aacattccct gttccacaaa
     2401 gaggtgtata aactatctta aagcttttac ctacattttt aactaaatct tttcttataa
     2461 tttggctttt tactacttct ataaatttag tatcttgtga ttcgtccaac aaaactacca
     2521 aatttttatt agcttccgtt aaggttggta ttgtactata atcatgtata ctatttactt
     2581 tctctgttat tgcatttgca atctctggca taacttgtgc tccatcttcc cagtatacct
     2641 tatatccatt atattctgga ggattatggc ttgctgttat gactatacca gcgattgcat
     2701 ttaagcttct aacagcaaaa gataattctg gagtagttct caaactatca aatatatatg
     2761 cttttatacc acatgctgcc aaagtatttg cagattctat acagaattgt ctagacttgt
     2821 gtctattatc atgagctatt actactcctc tacttgtttc ttcttttgtt gtgttttcta
     2881 atatatagtt tgccaatcca aaagtagctc ttctaactgt gtacttattt attctattcg
     2941 ttcctgcttc aattattcct ctaagacccg cagtcccaaa ttctaaatcc ttatagaatc
     3001 tatcctctat ttctttttca ttatttttta tattctccaa ctctagcttt gtatcttcat
     3061 caaaatatga accctttatc cactcttcat aagttttagt ataatccata ataaacccct
     3121 ctctactgta tgtatttatt gcaactgacg cataaacaag cattttaatg taatctataa
     3181 ctcttctata tacttattaa ttgaattaat aaaaatgtct gccattattc tatgaccctc
     3241 tttggttgga tgtaacccat caaaatataa ttttttatat ccttctagtt ccatattttt
     3301 attatattct gaataaaaat ctacgaaatt agtattaaat gtcatacaaa atttttttat
     3361 ccaataacta tatgattcta agttttgaga tactatatta aaatcagtaa aacaactcca
     3421 atcttcccta acatttggaa tatctggttt taatggtata cctattatag gtataatatt
     3481 ttttgaatat gactgatgca ccatagccat tatattagaa ttaataattt catttcccgc
     3541 accagcaata aaatcattgg tgccacccat tataaaaact atatcaggag agttttttac
     3601 aacatcttca ttaaacctag ctaacatacc acttgttgta tcaccattta tacccttatt
     3661 tactatttct aatcgagtct ctttatttac aatgtttgtc catgaattac ttcgtgatac
     3721 tccaaatcca tacgttaaac tgtctcctaa gcagactatt ttcatcttat ccccccttag
     3781 atatattttt atgttttaag aataatctaa cagtttagtt aattcatatt ttatactttt
     3841 ttgtccttca agtaaagtta ttctattcac taatttaatg tagatatata tctatacttt
     3901 tccttcccca atttgaagct tttccttcac tgttcataaa aatgtctatt ctattacctt
     3961 taattgcacc tccacaatct tctgctacaa aagtcatacc cagtttaggt atatatactt
     4021 ttgttccata tggtattaca cgtgggtcaa ctgcaataac accccatctt ggagttgtac
     4081 cagtagatgt tattgtatct cctgcatatg cagtggcaac cactttcata tgtttgcctc
     4141 ctcttatatt tgctcctctt gatgctatta gtactttttc tttagttcca tattttataa
     4201 tttttgtagt tggctcctta gatattactt cttttgataa gacttttttg actaactttc
     4261 catcatggta agttaaatta tagactagtt ttttttctcc ttcttgacct tcttgataaa
     4321 ctttagattt tccttttaac aaattactat catttacatg ctttgtatca aatggaattt
     4381 tttcaatctc ttctttttta cttttagtta ccttaacaac ttttattttc atatcatctt
     4441 ttagttctgt atctaaactt ggattaatct tatcttcaga attatacttt ataccctgtt
     4501 catccaaaag ttcctcaact gttttcttaa aggaagatac tttttgctct tgacccttaa
     4561 cagttaaaat tatgtcttct ttatttaata tagaatatat acttaccaaa cccattaaaa
     4621 ttgatacact taataaagat gatattatta tcctcttttc tcttttctcc ataataatct
     4681 ccttccatgt tcaaatttta aattaatatt attcattaca gttttaacct tatattatat
     4741 atgtatatta tagctttcca aatttatgta acttttttgt aacttctaaa attattaaga
     4801 ttacttttag acctagaaaa gtccataaat atactgtttt tagattatca aagcttaata
     4861 taatagtttt tagttatgtc ttaagcaatt atctcttagt ctatgagata taagcataaa
     4921 taaaagaaag aaatgacact caacttaaaa cacattgtat gcaaatactg actatggtaa
     4981 aatgaactca taaataaatt ggaattttcg tgattaggag gagtaaagtg aaatttgaac
     5041 ggttaagaac cagtgaaaat aaattatatt acaaggctat ggaattatat aaaattagtt
     5101 tcccatttca tgaacaaaga aaatcttctt tgcaagaaga aattttgaaa aataaagaat
     5161 atcaattcaa tttaatttat gataaaaacc agtttgtcgg cataatttta tgttgggaaa
     5221 cagatgactt tatctatgta gaacatttct gcatttttcc agaaatgcgc aataatagat
     5281 atggacaaag agcgttagag cttttaaata agaaaggaaa aactattatt ttagaaattg
     5341 accttccaat tgatgagatt tcaatttatc gcaaggcgtt ctatgaaaga gtaaaatata
     5401 agacaaataa ttatgaacac attcatccac cataccatga aaaatttaat gggcataacc
     5461 tgattgtaat gtcttaccca gaaaagttat ctaaaatgga atatgataaa tttaatcact
     5521 atctaagaaa taatgtcatg agtttctaaa tataattcct atttattgaa ctgtataaaa
     5581 cttcataatt aaataaaaga gatgtctcaa aatagaaata agactctttt ccattgttta
     5641 aataataaag atatattgga tttcatacaa agaaggatgc atcatgaact aaaaaattca
     5701 tgatacattc tctttttcac gcttaaagtt agaaaacagg tgagatttta tagacaaaat
     5761 tacattttat ataacaaact ttattctatt tttataaatt taggtttcta tttttgtgta
     5821 ataatacaga taattcagat ttctattttg aaacctctat ttttctaaat aactgtttaa
     5881 gacagacaaa aaaggtgatt caaattttta ttttgaaaca ctttctttta atttgattaa
     5941 tctttaagtc ctatattaaa tttttactta ttctttattt ttcaacattt tttccatatc
     6001 taaaatcttt tcaattacat ccttaaaaat tggttcttct ctaagctcat tataactatc
     6061 atcattaagt acaattaaca agtttgtata tatattaaaa gtatataaat ttttagactc
     6121 taccttatca aaaaatctca tttgacttac atccaaattt tcatgtatag gatatttttc
     6181 taatatttct tttagattat ataaggcttt ttttgcttct tctttcatgt taaaaaataa
     6241 aaaaattaga gataattcca tataatccat atacatacta aaaaatgcac ctttatcttc
     6301 actgagcatt ttttttattt ctaaagaaag atttgcatac cccagcaact tattttttat
     6361 atactccatt tctctctcaa ttgcattttt attaaaattt ctattctcca attttttctt
     6421 taattccttg tattgatttt tgtatacatt aattattccc ttacaatcca tgcttatatt
     6481 aaatacatta ctaaacaatt tattttgaag caatatttct gcttcttcaa gtctattttg
     6541 ctttaaatat atcatagcta aattaacact tgtatcccct attgacttat gtatcatatt
     6601 taaatacaac tctgcctttt cataatcctc caacatacaa tatgcgtttg ataatataat
     6661 aagagctgtt tcttttattt ggatatcatc tgtatttctc actatatctt caagaatatt
     6721 cgttgtttct tttactctgt cattaagttc actcttacca aatttatatg aatacatctg
     6781 atacagcgaa catatcctta gcttcaataa atagctttta ggatacttct ctatatacat
     6841 attggaaagc ttttttacag attctaagtc ttgtctaaat tcttcttttg gttcttctgt
     6901 tgataaatca atttcaattc tagcaaagcc tgattctaat tctttataaa ttttcattac
     6961 ttcttcttca cttaaatcaa tcttgtaatt taaaagctta tcaatagaca cattaaaaaa
     7021 gtctgctaat aaaggcaata attctatatc tgggtaagaa tttccactct cccacttaga
     7081 tactgctggt gttgacacac ctataaactt tgctaattgt tcttgagtta aatttcgttc
     7141 ttttctaaga cgctgtatga ctttacctat tttaatttgc atatatacct ccctagtaca
     7201 tctgttgtaa tttaagtata ttaaaaaaat atttactagt aaatcgaggg aaagttaaca
     7261 aaatgtctgt aatttaacca ttggttatat tttaatctaa aaccctatct cattggcttg
     7321 aaatataaaa tctattaatt aagtttaagt ataacctaat taatagattc acattaaata
     7381 gcatttaaat atttagattt tctctttact acaacttact atatttaaac tttcaaggaa
     7441 ttttaaataa aataaattcc atatacaatg ataataatac tgagtgttaa taatttattt
     7501 ataataacac ccacctattc aataaatggc attatagcaa aaaatcactt ctgggctctc
     7561 tcttttctac tttgcatata cataatccac ttttcaaata ttatatataa ataaatttaa
     7621 agcaaatata tcattatatt tttttattac aatatttcta ttttattatt taaccaacaa
     7681 attatatcct caaaaacttc ttctctattt atttcattca acaattcatg ccttccatct
     7741 ttgtaaagtt tgcaagttac atcttttact cctaatttta tatatctatc ccttagtctt
     7801 aagactcctt taccattttt tcctatgggg tctttatctc cagatattat gtaaataggt
     7861 atatcaagtg gcactttttt taagttttct ttatcctcaa tttcttttaa cccttgaact
     7921 aaatcataaa aaaagccaca actacacact actccacaaa atgggtcatt tatatatttt
     7981 tcaacttgtt ctttatctct acttaaccag tcaaacttag tcttttcatt tcttcttatt
     8041 atttcgccac caaatatcaa attatttatt ttattacttt tagaacgtct tccatacttt
     8101 ttaatctcac gattgattat cagatgagca agatttagaa tgattccttg ctttccattt
     8161 gaaccacata gtataagacc agataaatta ttactgtaat ccattatata tctttgactt
     8221 gcaaatgaac ccatgctgtg tccaaataga taaattggca aatcttcatt ttctttcttt
     8281 ataatatttg ttaaagtata catgtcctct acaagacatc taaatccttc tttttcagct
     8341 aaatgtccaa cattttctat tatctttgca gtttttccat gacctctatg gtcattgata
     8401 tatactatat aaccattttt agtaagtacc ttggcaaaag tctcatacct ttgtgcagtt
     8461 tcagccattc catgtgcaat ttggataact gcctttggtt tttttatatt ttcatcttcc
     8521 catttatatg tatatatatc taaaccttcc tctcctttga aagtaaagtt tgtacacttc
     8581 ataattatcc ctccagattg attttattca gtatattaat ttatatatta tattacaagt
     8641 attttcaagt caaaatcttt ctaaaaaatt taagaattat taattcctca tagaaattat
     8701 cattggtaca tataagtttt tacataaaat tatacaataa tttaattaat taatagttac
     8761 ataaaaaagt aatactttaa tatattttga caccatattg acattagaat taattcatga
     8821 tattctgttt tcgacaaaac aacatctaaa aataaaggag atatatttca tggattattc
     8881 aaatgaacta aaagaactct ttttaatgaa tcaaacatat gcaactcttt ttacccttac
     8941 aaacaaaata caaatagaag gagataaata ttttggtata cttacttcaa gacagtatat
     9001 gactatttta tcaattcttc atttaccaga ggaagaaaca acattaaata atattgcaag
     9061 aaaaatgggt acaagtaaac aaaatataaa tagactagtt gctaatcttg aaaaaaatgg
     9121 atatgttgat gtaattccaa gtcctcatga taagagagca attaatgtta aagttacaga
     9181 cttaggaaaa aaggttatgg taacatgtag taggactgga ataaacttta tggcagatgt
     9241 atttcacgaa tttaccaaag atgaattaga gaccttatgg agtttactaa aaaaaatgta
     9301 tcgttttaat ggtgaagaac aagatggctt tgaagaagat gctaatttta tggaatatga
     9361 agaaatagat aaaattaaat ctgaagcact tgaagaattt gcaaaaagaa gaaagagagt
     9421 aaataaaaac gattaatact atcgcatcta taatactact tttttatagt acaatatttt
     9481 atataactat aatgtcattt ttatttttta acaaaacaat cattacatag attaggtgtt
     9541 ctcaggaact ctttttctgt tttttgtaca aaaaaaggag atgattagac ttttatttta
     9601 aagcatctcc tttttcacta ttatgattat tctactccta aaatcatatc ttttaatttt
     9661 tgtaaatcat taattggttg actacaacta ttactttgac acacgtaata actaatctta
     9721 tcatccttca atctatagtt atttaaaaaa cctataatag tgttttcttc attgtatttc
     9781 tttgctaaaa aagttgcatt tggtataaaa ttttcactta tcaattcttt aaaggcaatt
     9841 aaatcagaat cttccttaaa aatacatata atttccttag tagaataaag ttcaaacatc
     9901 aatgaaagca tataaaagct gtagccagtt ggagaactct ttacattatc cacatacagt
     9961 tttaattgtt tatatgacat ttcttcaagt ctattatctc cagtaatttt cgcaagtctt
    10021 ataagattgt ataattgaac agaatttcct gatggaatag ctccatcata caaatctttt
    10081 ggtcttgcta ttaaattttc actatcttta ccatatatat aaaatccact tttttcataa
    10141 tcccaaaaaa ggttgataca gctttcattc aaatttaaag ccttttctaa atatttcata
    10201 ttaaatgttg actcataaag ctctatatac gcccagatta aaaaagcata atcatctaaa
    10261 tatgctaaat agtcggaact tccatctcta tatctagcta ataatctacc tgactcattt
    10321 acaagattat tatttataaa gttaagacac ttatttgaat actctaaata tatgtcattt
    10381 tttaaagtac tatatgcctt tgttaaagca actatcatca atgcattcca tgatgtaagt
    10441 atcttgtcat ctttatgcaa agatgttctt tcttttctat attcaaatac ctttttacta
    10501 aggtcagcta tcttttcatt atgtctctca tattctttat tttttatgag attaggaata
    10561 cttttgcctt caaaatttcc actactagtt atatcaaaat aattattaaa gaaagttcca
    10621 tcttcttcac ccaaaacttc aattatctca agaggattaa atgtataaaa tttaccttct
    10681 tcaccttcac tatcagcatc ctgtgctgaa taaaacccac cttctttatc cttcatctct
    10741 ctaactacat aatctatagt ttttatagca atttctttat acaactcttt ctttgttatt
    10801 ttatacgcat ctaaaaaagc tatagtaagc atagcattat catatagcat tttttcaaaa
    10861 tgaggagcca accacttttt atcagtagag tatcttgaaa acccaaatcc aatatgatca
    10921 aacaggcctc ctctatacat tccatctaaa gttttttcca ccatttttaa cacatcttta
    10981 tctttttcta tactataata tttcattaaa aacataagat tctgaggact tggaaattta
    11041 ggagcattac caaaaccacc ataattttca tcataaatag ccttaaatac tctaactgat
    11101 gaactcaaca tctcttttga taaatcaccc tcagtatttt ttacaccaaa atcatcctta
    11161 agagcttcta ttatttcatc tccagatttt attaatatat ccctgcttgt attccatttt
    11221 tcagatacat tctctaataa atctataact ccaggtctat tataccttga atattttggg
    11281 aaatatgttc ccgcaaaaaa aggtttttta tctggtgtca ttattatggt cattggccat
    11341 ccaccactac ctgtcatagc ttggcaaact gtcatataca cgctatctac atcaggtctt
    11401 tcctctttat ctacttttat tgccacaaag tttctattca ttatttcagc aacttcttca
    11461 tcttcaaaag attctttttc cataacatgg caccaatgac atgttgaata tcctacactt
    11521 aaaaatattg gtttatcctc ttcttttgct tttttaaaag cctcatcatt ccaactatac
    11581 cagtttatag gattataagc atgctgtaaa agatatggag atttttcatt tattagatta
    11641 ttaggttttc tattactgtc caaaatacca cctcatttgt tattttatta tatgttttgc
    11701 aataaacaaa tagatataca cttataataa gtgtataccc atttttaact atacatattt
    11761 aatttttcat ataattaatt cttctaataa ataattttta taactactaa aacaaaacaa
    11821 cataaaaaat atcaaattat ttttttaaag gtttttcaaa agaaggagga ttaatatcta
    11881 tcttagtatc gtgatttggc cctggagtag acaaatcgaa atataattta gaatcttctg
    11941 taactccaaa atctctattt gaacctgtat ctttaacttt atttaaagca tctctaaata
    12001 gagcattatg tgcttcttct ctattaagta agaaatctat tgtttctttt acatatttat
    12061 catctatttg tctatacaag tactcataaa ctaccttagc tctttgttca gaagctatat
    12121 ttgaaagtaa gtcagctact aaatcccctg taactgtaac gtaattagca gtccaaggag
    12181 ctcctgatga gtttattaag actggtgata gaccagttaa tacatgtgtt tctatttcgc
    12241 ctgtattaac tgaattataa tcaacatcat gtccatttaa taaatttata gtttgtgcta
    12301 ccatttccat atggctaagc tcttctgctg ctatatcaag aaataaatct tttatttgag
    12361 ggtcttttat tctaaaactt tgggacaagt attgcatagc tgcttttaac tctccatttg
    12421 caccacccaa ctgttcttgc attaaaacag catattgagg atttggtctt tctactttta
    12481 cttcttttaa taatgcttta tcgtgtttaa acatatgaat acctccgtaa attattattc
    12541 aaaattatta tttacttaag ttgtaatatt attctaggtt atttgtttat tgttgttatt
    12601 ttttacaagt gttggtattt caatgtttta ttaaggtcag ttttgttgtt tatgttttat
    12661 tatgtttaat ttaaatttat tctattattt attttcggtg gcaaaagggt ggcaagtttg
    12721 gttttgccac ccttattttt atttgtaaaa aaatatagtt tgtcaatata actttattga
    12781 aaaatatgga aataaatatt gcttttgccg gttcggcata ttataatata aatatagaaa
    12841 gggggtgaaa aggaaaatga gtagaataga aagaagaaaa aaaaatagag aaagcaaact
    12901 cgccaaagtt aaaactgctc tctctatctt acccatttta ataaatttaa ttgataaact
    12961 aattaaatta ttaaaagatt tatagttttg taagctagga agaatttaat cttcttccta
    13021 gtgacttctt tctaaatatt ataacacatt ttctataaaa tatgagtgat aaagtttata
    13081 aattctgtct tattatagtt gctataacta tagtaataga cataatactt ttatttttac
    13141 attttaatat caacaatata ataggattaa tagtaagcat tttattgtta atatttgtta
    13201 cattacaata taaaaaagga ggaaaatagt ttggagcaaa aagaatctaa aatagtttac
    13261 agtaaaggtg gttcaggtac ttattcggct aaaatttctt tacctttaag ccaacttgaa
    13321 aaaatgggat ttacaaggga aaaacgtaag gctgtagtta tttttaagga agatgagatt
    13381 ataataaaaa aatctgatga ggaataactc taaaataagt gggtgtataa tgtaaacatc
    13441 cacttatttt tattatattt tgtattttat attaatatat tttataactt tattgaaaaa
    13501 tatggaatat ttattggctt tcacgtcacg tgatgatata atataaatat agaaaggagg
    13561 taagaaagtg gggagattag aaagaagaaa aaataaaaga gaaaacaaat ttaataaaat
    13621 taaaaatgct ttctctttca cattagtttt aattaatcta gtactcgcaa tactaagatt
    13681 gattaaagaa ttataagttt caatcctagg aggaaaatac cttccttcta ggtagcttct
    13741 ttctaatatt ataacacatt ttctaattta atatgaataa taaattttat agaatttgta
    13801 tgattattct tggtatcact atagttttaa atattattaa tataataata agttttactt
    13861 ttaaagctct tattgctttg gtatttactt tattattgct ggcatttatt aatagtagaa
    13921 aggaatgata ttattggagc aaagaaaact tagaattgga tttaataaaa gtggcaaagg
    13981 ctcttttaca cccagattga tacttccaat gtcatggatt aaagagatga atatctctcc
    14041 tgatgaaagg gatgttcttg ttacttttga ggatggaaaa ataattattg aaaaaactga
    14101 aaatgaataa aaaaaggagg tagtaactat ttccagttac tacctttcaa tctatttaac
    14161 taagttttct acttcattaa atgctttttc atttccattt ccaccaattt gagttatatt
    14221 tttaaacttt ttattcttga ttaactcttt ttggctatta tccaatccat ttccaactaa
    14281 tactactgga gattcagttt tacctgcaag cactcctact gataaggcat ctactaaatc
    14341 gtcttgttta ttcattccat tcttagctac atataagtca ttaagtgtat ctttgctata
    14401 gaaatgactt attacttttg agttagtttc tgttctatca gaacctgcta ttttagttac
    14461 agatggtaat gtattattca cttctttatt attaaataaa gattctccac ctattacata
    14521 tgattttgat atgtttttat tagctattaa gtcttttata tctttcatat catcattttg
    14581 attagtaaga agaattggca tttcctcttt tgctgatata gctcccatac ttactgcatc
    14641 agctagtccc ttttctccat ttaccactac tacttttgac aggttagaat ttttgtctaa
    14701 ttcttttgca agttttattg atgtttcata tctatcattt ccagatattc taattacatt
    14761 taatcctttt ttctttaaat cagacactac tttttcatca acagacttta atccgcccac
    14821 tatgtacaca ttttttgctt ctaatctatt tatttctttt tctgttaatt tatttaagtt
    14881 attattttta gtaagtaata ttggtgcatc tttagactta gcaaatggtg ttgctgataa
    14941 agcatccgat atgctagagt catttattaa cactatatta tcagctttat tccatccttt
    15001 ttgacttatt ttcactgatg tttcatttct gtctacacct gttaattttt ccttagatgg
    15061 tgttgaagta ggttcagagg actcttcatt tgtagatgat gaacctcctc cactccctga
    15121 cgaacctcct gttgatgttg attttttttg atttgaccaa tctaattctt ttaattttgt
    15181 gtttttgctt acatctaagc tggttaatct attttcccca caatatagcc ttacaagttc
    15241 tgtatttttt gttacatcca agctagttaa ttcattttgt gaacaatata agtatttaag
    15301 atttttattt ttacttgtat ttaaactatc taatttattt ttataacaag ttaaattttc
    15361 taagtcaata tttttactta tatctaaagc ttttatttca ttgtcataac agtataattc
    15421 taaaagttct atattattct ctatattcaa atttgttaac ttgtttgcta cacactctag
    15481 atattttaaa ttcttattgc tacttatatc caaattcttc agattacttc tactacaagt
    15541 taaatcttcc aaattaatac aattttccac atttaaagta cttaattgtt cttgattaca
    15601 atatagtttt acaagctttt catgattttt taaatttaaa ttttgcaatt tattgttgtg
    15661 acaaattaac ttaacaagtt ctttattatt atctaaattt atattttcta attgattaaa
    15721 agaacagttt aaatcctgta attctaaatt tgtagatata tctaaatcat ttaaattatt
    15781 ataatcacat ttcaaaattt ttaatttctg attcttagat aagttcaaac tagtcaattc
    15841 attttcaaaa cataaaagag ttgtcaaatt agttgcttta gttatatcta aactctttaa
    15901 attatttttt ctacagttca attcgtctaa tttagaattt tctataacta agtctgttag
    15961 attattttct ccacaatgca acgttgttaa ctctctatta ttacttaaat ctagacttgt
    16021 taattgattt tcgttacaat acaattcttt taaatttatg ttgctactta aatctagact
    16081 cgttaaatta tttcctctac aactaaaatt ttctaatcca ataaaataat taattccaga
    16141 taaatttttt attgatttgc tccctaaggt taaacctttt gtagattcta taatcgcttt
    16201 ttgtgaaatt ccaattggat ttccatcatc taaaatactt agtttatcac aaattacttt
    16261 tctaaaattt tcatctggaa aagcttcttc cattgtcata ttttctaagt tattagcttt
    16321 ccttgtttca tatctaactt ccctattttt ctcaatatct tgtgcattta tcttatgtac
    16381 caaaggtgat acaactacca atgaaagtga taaaataact gcttttttgc ttaaattttt
    16441 catccccaca cctcattcaa ataatattta cccacttgaa ttgtatcaca taatattaac
    16501 cttagaaaca aactttaata ttattatata ataaattatt tttttattat gaatgaggca
    16561 ttagaagata acaactactt ttagttgcta ctttttaatt tatctattaa taaaatctaa
    16621 tgccttataa agtgtatcaa atctatcatt accctttatc atagtaaatt tttctttagt
    16681 catagaacca atcttctcac atgctgcacc accaatgaca taaagatttt gcgtctgacc
    16741 tggcacgtaa tcttttatat cacatatcag tattttccca tcattataac cccaaccaac
    16801 tacagttgca gagattttgt caacttctcc atcataaaca attgtatgtt tgtacatctg
    16861 tttaactccc tcattattta tatttttatt taatacacct tctacaatta acttagcaat
    16921 accttcatga cctagtttct tagctttctc ataatcttct ttattatcac agaagaaact
    16981 ttcaattaat actgctgtag gatttgaact atttaagata tataatcctt tatctaattt
    17041 agctcttcta tttctaaata ctgtacctag cttattacat attctagttg catattctaa
    17101 acctttatta ctataatata gaacttctga accttttcct tgaccatcac ttgcatttag
    17161 atgtagttct ataagtaaat catatcctcc actattaact ctaggtattt tataagtctt
    17221 ttcttctgct ttagttttaa actgcttttc agggcatatt attacatctg ccttatgacc
    17281 ctcttttcta aatgtatctg ctaatactgg tgcaagagat ttattgtatt ggtattcgtt
    17341 aactactcca tcagcagaag tgcatgcacc actttttaaa atactgtgtc ctactgtaat
    17401 acatattttc attatttatt ttcctccttc ttttcaataa aaaaacactt acatatagta
    17461 agtgtttata aacttcttaa agtaactatt ttatttagcc aatatctcta tccaattatt
    17521 aggaaaacca ataagcttta agtctataga attattataa tcattaatta acttttgaag
    17581 ttttataaaa aaactattcc aaattaattt atccatagtc aaatgcttca tagcaagtat
    17641 ataagaaaat atctttttat tatccaagtt atacttttta tattcgtttt ttattgttat
    17701 aatacggaaa ttattattat atattctacc ataatgagcg cattgatttc tgacctgtgt
    17761 taatgattgt aaccaagatt taactaataa agtattcact ctacacaaat tattttttat
    17821 gtatctagta tcttctggca acatgtttga atataatttt gacaacatac caaaagtcat
    17881 tatttctgtt gcaacccaaa tgggaagttt tccttcatat ttttctttat ggtgtataat
    17941 aaaaagctta tctgaattat tatttttttc tctctctaat gctgtcaaaa aattaatata
    18001 gaatttttca tctttgaaac tctccctttc tagatatcca caagcaccat gtttaattgc
    18061 taatgtatat gcaatgtatg tcctaaatgc tatttctatg ctccctaaca aatctgttaa
    18121 taatatccta aattccttat taaacctata tatatcataa acttcttcaa acgtagttcc
    18181 ttctttatat gagccatcat cattcttaaa acttagaaga tatgctgtaa acctataata
    18241 attgacatta cttaatacaa atttagcatc ttctttatca tttattatta atcctctact
    18301 ttttaaaata tctatttgtt catcaaatgt tttttcttct tttacttcaa ccattgtttt
    18361 caccttttca tagatatact tttatataat aaaacccacc aattaatatt agtgggtaaa
    18421 tttatctgtc ccgcctattt gagctataaa agaagcttgg cgggttcctt caaaaatcaa
    18481 tgtcccgtat atttgagcat ataaatgctt tacgagttct gtactattat tatatactta
    18541 ttagctataa aagtatactc tatttaatga aattttttaa atttttgtga gtaataatat
    18601 ttttgttaat ttcattactt ttcaccatcc ttcaactgtt tgtaagtttg atttatacct
    18661 attgcaactc cccaacaaat tacaccttgt aagactgcaa caggatttaa tcctagcatc
    18721 catattgaga aacctattcc aagtatcagt aacactactg gaatgtattt attatctaat
    18781 tgtttatatt ttttacaacc tttacctata atagagagag cagctactaa aattagtaac
    18841 tgctctggta taaaacttat taaattatcc atatcttaac ctcctaatta attaaaatat
    18901 tcctctttgt attgcaaata taaagaaccc tataagtgtt gtaatcattg taccaattag
    18961 ccatttgagc atacttgtaa gtgagtttag attctcacac aatgctttta attctgcttt
    19021 agactctata tttgctattt ttaattcgtc tatttcttct ccatgtttat ttattcttgt
    19081 ttcatgtctt tttaaatctg cttcgaaaag ttcttcattc atgtaaacct ccttatttgt
    19141 attaaaatag tacttagaaa ttctctaagt cttttactta taacaagcaa taaaatatcc
    19201 cttaaatgga gatttacctg ttgtatagct agtacttcct gctttaaatc cttttctatc
    19261 tgcattaatt tcaatataag cagtactacc acctggatta ctttctaatt tttgttttaa
    19321 tggagtataa tagcattctt tttcgttcat tattgaaaat actacaagat gagtaggaac
    19381 agaagggaag gtaaccttat atggctttgt ttcttctcct tcgaaagaac ctgcacttat
    19441 tttatatctc atattaactt gttgctgtaa atcagttata gtacctttaa actctgtaat
    19501 atagttcgca tactcttgaa atgtttgttc tgatgtagca ggagagccga tagcagttgc
    19561 aattaactct tttccactat cggcttcttg aaaagcctca tctgctctat ttatacattc
    19621 tttcaatgct tcttctacat tttcacttgt aaacttattt tctgtatctt ctatagttac
    19681 tttctttgct tctaatataa gatttctaac tttattaact aactctttaa aagtcattta
    19741 gtcaccttct ttcaataaaa aaagaaccct ctatatagtt ggttctgctc cttctactac
    19801 tccactattt tttataatat aatcctctat agcctttctg tactctgtgt tagtcacgtc
    19861 atctaactga aattctctat ttttcaatga gtttaaccct ccgtttaata ttctctctgc
    19921 taatattctt actacaacat tatttatatt cattataata gtcctcctga tttttcattt
    19981 tctgcaatca atatttgatt ttctaattct tgtattctct tttcttcttc actcatatag
    20041 attggtattt cttctaaaat tggctgttta gtctctatat ttataccttt tattttgtac
    20101 ttatcataat caacataatc gtattccaaa tctatatatt ctattttttt aatatttttt
    20161 cgctctggaa catttccttt cgattgtcct tcatagaata ttatttcgcc ttcttcatca
    20221 aaaaatattc ttcttcctac ttctatatat tcagtcatag tttcctcctt taacttattt
    20281 tttaagcaat tgcatagaaa aaatgtggta ttttgtaatc tttcactggt aaaatacaag
    20341 tgtgacctac tacacaatcg aatgtgttag gcatgtacat ttcaccaaaa ttgctaggtt
    20401 gaggcactgc tgttataata ttacgaaaag atgagtaaaa aacaaaacta cacaaaccta
    20461 tattgtaagc atttctatag acaataataa attttggagt aaaagctaag ttaaaactta
    20521 ttgcgtttgc tgaacaagtt tttggcgtat tattatctac ttgatagagt tgaaaagttt
    20581 tttctccaac ttgagtacat tctccttgtg caataaaaat atttgcgatt ccagctactt
    20641 taccaattag tgaatgaagt gtttcagttg attgagcaga tatatttttc atagacaaac
    20701 taattgctag tgtatttttc aaggtctgta tttttgtttt agttacactt agcttatcag
    20761 ttccaacaaa aggacttcct aatgtaccag ctatattatt tttaccaatt tgcaattcat
    20821 ctttcacgtt ctcaaaagtt gttattaagt cacttagact agcattttcg tttaattttt
    20881 ctatcataaa aatatcacct ttctattttt agtaaccatt tttaaagaat aggttacttt
    20941 taaagtagaa agatgattaa atttgaacgt ttcttttaat gtagaactta gaatgatttc
    21001 tttataagta tgtgaaagta agtaaaataa acaaaattaa aactttttag taacctattc
    21061 tttaagaaga gttactaaaa agttgctatt ttatttcttg ctctattgta tttatataat
    21121 cctctactgc ttttctatac tctgtattag ttatatcatc tagtttgaat tttctatttt
    21181 ttagaggatt taatccttca tttattattc tttctgataa tatctttaca accacttcat
    21241 ttatattcat tataaaatac ctcctccaat ttcttgattt actgataata ataattgatt
    21301 ttctaactct tgtattctct tttcttcttc tgtcaattct acttttattt cttctaaaat
    21361 tggttctttt gtttctatat tcatacctat tattctatat ttttcatagt ctatataatc
    21421 atatggtaaa tctatatatt ctattttttt aatatttttt ctttcgggaa catttccttt
    21481 cgattgtcct tcatagaata ttatttcgcc ttcttcatca aaaaatattc ttcttcatac
    21541 ttctatatat tcagtcataa aatcacccct taacctatag cgtaaaaata aaaactagaa
    21601 ctaccatatt tactgtaaac tataggtaaa atacatgttc ctttttgaac ttgtatatta
    21661 ctatcgcttt tatcaagtaa ataagtcgta taactttcta ctgttgaagc aataaaagga
    21721 acataactac aaaaatagct agttgtaaaa tatttactag ctccgccagt attatacata
    21781 acgacgagtt tcggataaaa atttaagtta attctaacgt agtaaacatc tccattcgta
    21841 ctaattgtat atcttttgca tgtaccttta ctatcaataa tacactcgcc acttgctgta
    21901 tatattggcg taggtatatg tatgttggca actttattaa ttaaagacaa taaagactct
    21961 gtattaccac tatttacgcc tttacttttt aaattttctt taaaagtgtt cataagattt
    22021 tgcattttac cctcaaactc tgtaaattta gtactttcca aaaatggatt gcctaacaaa
    22081 tttgtaaaat tatctttact tgtttttaat tcttttgtac tattttcaaa agtggttatt
    22141 aagtcactta gactagcatt ttcgtttaat ttttctatca taaaaaatat cacctttcta
    22201 ctttcttagt aaccatttgt caagaatagg ttactttttg aaatagaaaa gtgattgatt
    22261 ttaagtaatt tttacaaagt atatatagta taaataattt tatttttaat agaaaaacaa
    22321 gcaaaataaa caagtataaa gctttttagt aactctttct tgacaaatag ttactatata
    22381 aaattaaaaa aggactatac aatataatcc tctccaatga tatttttata ttctattgtt
    22441 gttatcttat tcttttctac tgctgtttta acttgttcct tagtccataa ctcatcttca
    22501 tagtattttt ttattatttt gtaccacatg ttatatcaac tccttttcca tcattgcaaa
    22561 agttaacgct gctgtttctt ctcttaatgc ttttacttct tcgtgtgtag ctatagttgc
    22621 atttttagaa tttttttcta gttcctcatg tgcttttatc tcttcttctt gtttcttatg
    22681 catttctaaa tatttttctg ctgtttctaa agctgtcaaa ctcttatcta gtttatatat
    22741 agatttaatc gtattaactg actctatatt tgtaactctg ttataatcat caagtattag
    22801 aaatacctca cttgtttgga aatcataatt accttttaaa atttcttctg cttctactcc
    22861 atttattttt tcttttttat cccatatttt tatcattttt attttctcct ttttttataa
    22921 ttctggtata aaacatttat acctactatt ctcacaataa tatataaatt tatcttttac
    22981 tgatacagct ttaagtagtg aatcatatgg aaatgtagaa ttttttttat catactttct
    23041 tgaaaatact acaccatttt gattataccc gcctacgtaa aaatatgtat cgccacggta
    23101 cactgtatat aacatattat taatacttgt tgcaaagcca aggtatacac cttctactct
    23161 tgtaagagta ccataagctg gtttgtagct acttatacaa gtatcccaat agctgcttgg
    23221 gtctgttgtt cctgttatac aaaaagtgta ggtatctgtt tttgaacata caaataacct
    23281 tctattgcga gaagctggta aatttgtttt agtcgaccaa gtgtttgtac taattttata
    23341 atattcacta atagcacttg ttactgtatc gtaatttgta tctgtataac catacaaaac
    23401 atgtatttcg tcggaataag tatttccaca tgttccaccc ctaggtgtaa tcatattaga
    23461 aatataagac caagtgtttg tctttatatt gtatctttga caacttggag cggctgaact
    23521 atcgttatat ccacctattc tataaatata accatcatga taaaccatta atgcatcatc
    23581 acaaactgac ggtcttgcta aagaattatt aacatttgtc aacgtatcat agcgtaaaag
    23641 cgacaaacct agtgtattct tacaaagaaa atatatataa ttttctactg ctatgcacga
    23701 aggatattgt tgtaaacttg aagaaaaccc atcaaaaatt atatttttag gtttatacca
    23761 gtttggtatg tctgtatttc ctagttcaat atcatcaact ttacttacca tattttgaac
    23821 ggtttcattt gaagaagttg aaacaccttt tttgattaaa ttagttgcta atgtactctt
    23881 gaaagtttgt atttttgttt tagtagtgct aaatgaatca gttgaaataa aaggacttcc
    23941 tagcgaacta gctaagttgt ctttagcatt ttgaaggtca ttttttatat tctcaaaaga
    24001 gtccataact tcttttatac ttgaatcttc ttctaatttt atagtcatta aattcctgcc
    24061 tcctttttaa gtatcctttc gtttagaaat ggttacttaa aaaagtaaaa gagatagaaa
    24121 tttaaactat tctaaaaaca gctttatatt ttaaattaat ttactttaat gtataaggta
    24181 gtaaattcaa agattacaaa agtattaagt aaccatttct aaacaaatag ttactaattt
    24241 tatagttctg gtataaaaca tataatattt ccatttgaac aataatatac aaatttatct
    24301 tcaactgaaa cagctttaat gtcataactt gatggtattc ttaaatctcc taaatttctt
    24361 tcaaaaattc tcccattttc cccaaatgct atggttgtgt acttgccaga tgaataataa
    24421 tcgtaattac ctaaaagcaa ataatttttt ataggtacaa caaatgaccc tgatgtacca
    24481 tatctaccac ttatagttcc agctattgga ctataattag ctattattac agaataaaca
    24541 ttatttgggt cacttaaacc atatacaaca aaattatttg aaccttttgc acaagtgtaa
    24601 ccagcgtatt ttgaaagttg taaattccct ttattggtcc atgtatttgt atctacaaga
    24661 aaatattcag aggtattaac agctacactg tcagtagtat ttttctttcc ataaaaaaca
    24721 tgtatttgat ttttatatgc ttctccacct gctccaaatc tattagctcc catgttaggt
    24781 aaagaactcc aagttaaagt ttttacatta tattttttac aagtacttaa agctgtgcta
    24841 ccagataatc cacctatttg ataaatttcg tcattataac ttacaagtaa aaaattagtg
    24901 ttatatgctg gagatgcaag acttgtaaga gaatttgcta tagtattata ttttctgaga
    24961 tacattgtat ttttgtcgta tttaataaag aaaaaaactt cttcgcctat agccgcagca
    25021 tagttatcat ttcttatata agaatcagaa gccgtggtta ttgaaatatt ttttggttta
    25081 taccaaattg gaacattcat atttccctgt tctatttttc ctacattttc aactaatatt
    25141 ttaaaaggtg tattacttgc agttgatacg ccttttttat ttaggtttga aacaaaagta
    25201 cttctaattg aatttaatgt attcctagtt gtatctaact tgtcattccc tgtaaagggt
    25261 gaacctagaa catcagctat attattttta cctacttgca aatcattttt tgcagtttct
    25321 aatgtatcta tcatttctct tagttttgca ttttcttcta attttgttgc catttttcac
    25381 tctccttcat taaatcatat ctattaaatt attcactata gttattcctt tagttctttg
    25441 accacttatt tctaccatta tttcctctaa tactccatct aacttatcac ttgtaaatct
    25501 atcattagca tctgtaatac ttatactggt atctataagc tgtatactgc taatagaatc
    25561 ctctattttc ttagacgaat aagtagtcat ttcagacact ctgttatcat ctacagttgc
    25621 atttataaaa tgagtttctg catttccatt tatcacataa acgtttaatt ctgaccttgt
    25681 ttcacttcta acttctatag aattatcatc tataatttta aagtttgtaa ctacattttc
    25741 ttttgtagta gcatctataa tatttacaac tattctctgt gttaataaac tatgtgttac
    25801 agttgctttg aatccatttt ctgcatcctc aacccaatca tcaattgtta ttatttgagt
    25861 agatgccaca tttgaaccac ctgcgattaa ttggtcaatt ttaatatttt gtttctcatt
    25921 ttctgtgtca attctagtat ttaactctgt tttagcagtt tctaaattat ttgttaattc
    25981 tgttttagtt gtatctattt tagtattaac agtacctatt ttagtttcta agtcttgtat
    26041 atctttgagt gttgcaaaga ttattgttgg gtcaatttta agttctatat tatttacatt
    26101 agatacaata agcacagttt taaccttcat gtctaccact gcaccttgtt ctatagaagg
    26161 tttataacac tctttgtatt tagaaatggc aattaaatta ttttcatcat ctaaatatcc
    26221 tatttctctt atcataaatc cgcctacact tgatggtatt aaactctcta atattataca
    26281 atttggtgca gtttcatctg tagttgtatt tccaatattg ccttcccata ccacgttttt
    26341 gagagctgtc tgactctcag ttggagtata ttcactccct cctccatcac caagttgaat
    26401 ttttacaaat cccactttat tacctgtgac acttgcattt gctatctttg cttttcctac
    26461 atctgtaatt atagtgtaat aacttttatc tatagccaat atatcacctc ctaaaatatt
    26521 gttatctctt ggtatccaac tccattgcca gttaatacat caatttctcc ataagtttct
    26581 atatctggtg gactccaagg gtatatagtt atttcttgac ccattagggt tgttatacca
    26641 aaattcatat aattgtcttt gcttataagc actctagtgt aatctaaagt catattacat
    26701 ggcttaatat tacttacaaa agaatgaact tcctcaaacc aatcttgatt tctagcatca
    26761 ctttcaagat gtatgttata agtagcatta tttatagtta actcataatt gccttctcca
    26821 actacattat ctagccagtt ccttaaaaat ctctctgagt aaggtagttt acttatatat
    26881 ttactaaaaa tcctaaacct tctatcttct aaactctcat tacttttagg agttatagac
    26941 attatctttt cccatctttt tataccactt atagttaggt cctctaaaaa ctggtcattt
    27001 gataggtcct ttaatttatc atataatgtt tttatttctt tattttctac attaaatact
    27061 tttatatatt cttctttatc ttgtagaatt tgtggtaagt aatttattag attaatctct
    27121 ttatccaact acctcacctc tcactactat actgttacta tttattgtta gattagattt
    27181 aacctcattt atcattgtat ttgcaatgtc taatactcca tcaatactaa gtaatctagt
    27241 ttcaatttga gatatacgga ctattaagtt ttcttcatct tcccaactca tgttaagttc
    27301 atttaaatag tcgtctattg cttcttctgc aattgatttt atattctccc aagtgtagcc
    27361 atttttgtat gttatctctg ctgatatatt tatagttgta cttacaacac ctgtaacagt
    27421 aactttatgc cctattggtg ctaatcctaa gccttgtcct tgatgtccaa ttgggtcaat
    27481 ttcttcttgc actaaattaa ctaaatcctc tgatggtact ttgaaattag agttaattat
    27541 tactaactta acagttcctc caccgtccca cacaggataa accttaactc ctccaacatc
    27601 ttgtattttg ttaacttcat ctttatagtt ttgtatattc ccaccaaagc tctgtgaatt
    27661 taggctatca taatatcttt gtcttaaact atcttctgac tcttcatcct ctccatttat
    27721 caagatttca gttagttcag cagtttcaag accatctata tattcaatag gtattagttt
    27781 tcctaactca aatataggtc cagcagtttc acatttcatt ttatatgttt tttcagatat
    27841 tctctcaatt gcaatataat tgtattctcc tagattaaac ctagaatcaa gtggaatatc
    27901 tatattaaac actccttttg caatggtatt agttgcagat agtggtgtaa ttcctcgttc
    27961 tttacatctt ttctccaagt aataataact agcagtatct acgaatgttt ggtctagtaa
    28021 ttcatccatt gcaatgtatg tttctgtaag ttctatagca acaggagcaa gagcattata
    28081 tattatagaa ccttcccttt tatcaaaagt atctggtaca ctatctaaca ttcttttaat
    28141 tatattttca aatgtcatta actcaaacaa ttatacactc accaccttct ctgcttttat
    28201 atttccatat tttgtatgaa ctgaaaatct acattgtact ttacccttta tattttgaaa
    28261 ctcaaaatta tctatatttt caatcctatc atcttgaatt agtgcttctg ttatccttct
    28321 ttcaagttct ggtattacat atgaaatagg ttctccaatt aaatcattta attcaacacc
    28381 ataatttcta gaataaataa gatgctcata cctttcagta tttaaaatta aaaaaatggt
    28441 ttgttttaat gcttctacat catcacaaat accatctact ctatttttct ctatattcaa
    28501 tttaaacgtc ttacttggtt cttgtctaac atcaaaatta attatcgata catcttcaat
    28561 gtcataatct aaattatcgc ttggtaacac cttatcacat cctatctaaa atcaagtatt
    28621 gttgccctcc ttgcatacga attaaaacta acttatctcc tatcttttta tctgtatatc
    28681 ttttaaatgt atctgtttgt attagaaaaa tttcaccaat agatagtttt tgttctatat
//
LOCUS       DACXMZ010000023        28647 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.023, whole genome shotgun sequence.
ACCESSION   DACXMZ010000023 DACXMZ010000000
VERSION     DACXMZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.023"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            208..558
                     /locus_tag="JD016_04045"
     CDS             208..558
                     /locus_tag="JD016_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAU4998798.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            577..2220
                     /locus_tag="JD016_04050"
     CDS             577..2220
                     /locus_tag="JD016_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAU4998799.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2298..4358
                     /gene="recG"
                     /locus_tag="JD016_04055"
     CDS             2298..4358
                     /gene="recG"
                     /locus_tag="JD016_04055"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAU4998800.1"
                     /translation="MLDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDR
                     NNLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIK
                     NTFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIM
                     SIIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEE
                     LLILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLE
                     KIMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFG
                     INVGLLIGSLAKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFG
                     VMQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEK
                     SKRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGL
                     LHGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQ
                     LRGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTR
                     QHGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKE
                     ISLN"
     gene            4417..4971
                     /gene="rsmD"
                     /locus_tag="JD016_04060"
     CDS             4417..4971
                     /gene="rsmD"
                     /locus_tag="JD016_04060"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAU4998801.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4973..5470
                     /gene="coaD"
                     /locus_tag="JD016_04065"
     CDS             4973..5470
                     /gene="coaD"
                     /locus_tag="JD016_04065"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAU4998802.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5474..5998
                     /locus_tag="JD016_04070"
     CDS             5474..5998
                     /locus_tag="JD016_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998803.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6342..8261
                     /locus_tag="JD016_04075"
     CDS             6342..8261
                     /locus_tag="JD016_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAU4998804.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITEVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNNEVKEDVVEEYIDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8274..8561
                     /locus_tag="JD016_04080"
     CDS             8274..8561
                     /locus_tag="JD016_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU4998805.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8694..9953
                     /locus_tag="JD016_04085"
     CDS             8694..9953
                     /locus_tag="JD016_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009109555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAU4998806.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            9956..10696
                     /locus_tag="JD016_04090"
     CDS             9956..10696
                     /locus_tag="JD016_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAU4998807.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10709..10819
                     /locus_tag="JD016_04095"
     CDS             10709..10819
                     /locus_tag="JD016_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998808.1"
                     /translation="MLMNFEVYQQRILIIYNKCYHLKAVGKNLQLFIIVD"
     gene            11052..12329
                     /locus_tag="JD016_04100"
     CDS             11052..12329
                     /locus_tag="JD016_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAU4998809.1"
                     /translation="MGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSKKDGEKEKLVLW
                     MPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISAGEGPDVGYMYA
                     EMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAANPGVLYYNKDI
                     LEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFGNLNWNWYPYLW
                     QAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEMIESVFGPGKAA
                     FTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSSAKNKELAYKLI
                     QHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPLVVGPHGVEIYE
                     YLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12427..13344
                     /locus_tag="JD016_04105"
     CDS             12427..13344
                     /locus_tag="JD016_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAU4998810.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSNTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            13360..14169
                     /locus_tag="JD016_04110"
     CDS             13360..14169
                     /locus_tag="JD016_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAU4998811.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14227..15975
                     /locus_tag="JD016_04115"
     CDS             14227..15975
                     /locus_tag="JD016_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HAU4998812.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKELDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVSTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     RYDKVKKYVLELLDRV"
     gene            16237..17259
                     /locus_tag="JD016_04120"
     CDS             16237..17259
                     /locus_tag="JD016_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HAU4998813.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGVTKEAPETSRRAWVKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDREILS
                     ALIEKR"
     gene            17264..19570
                     /locus_tag="JD016_04125"
     CDS             17264..19570
                     /locus_tag="JD016_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAU4998814.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSERRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYVLS
                     SLSLKIFSQNIRNVRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSITYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFHMYIKNIGKLRIVNFNYQYVLLNF
                     ENKNIYPKEIALEISEGIVLECDLCEGKIDERGILYRVNNIDSIMQNLFLRDEMMDWI
                     LQNKKILDSKPNEKKEKSIDEFEPMEYI"
     gene            19581..20657
                     /locus_tag="JD016_04130"
     CDS             19581..20657
                     /locus_tag="JD016_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998815.1"
                     /translation="MKTLKVFFCSAKYFILVFIFFINTTFISSATSDKILDLSFKKIE
                     TDLSSKITYEDTGVKIETNSSKSDKKRYLYIYQNMKENWSMYNNFYIEIQNKNKSSQK
                     INLSIQSKNMLEFRLKEGSEVFLEDKNIIYSDKIKEGCIEVPGEFEGKIYVNFNSLIN
                     EKSNVVLDSSMLSNIVSWGITFIPSDEEHNNIIIKKISLLSEEKLSFLHNIKITGNEE
                     VQIPVLGQGISQYEVLGLESDSKIKYSLIGKKDNLSISQKGKLTLNNKSKPGQIILQV
                     DVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMKKINFMNNIITFVR
                     ILFVSLVIICFGIYLYWKKCSKTK"
     gene            20675..22093
                     /locus_tag="JD016_04135"
     CDS             20675..22093
                     /locus_tag="JD016_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HAU4998816.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22108..23316
                     /locus_tag="JD016_04140"
     CDS             22108..23316
                     /locus_tag="JD016_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998817.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(23390..24778)
                     /locus_tag="JD016_04145"
     CDS             complement(23390..24778)
                     /locus_tag="JD016_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HAU4998818.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            25119..26453
                     /locus_tag="JD016_04150"
     CDS             25119..26453
                     /locus_tag="JD016_04150"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAU4998819.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(26582..27499)
                     /locus_tag="JD016_04155"
     CDS             complement(26582..27499)
                     /locus_tag="JD016_04155"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAU4998820.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27707..28591
                     /locus_tag="JD016_04160"
     CDS             27707..28591
                     /locus_tag="JD016_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998821.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
BASE COUNT        11197 a         3068 c         5089 g         9293 t
ORIGIN      
        1 ataaagtaaa tatatttgga aaataattat tatttttctt tgaatatata tactatagaa
       61 gaaattttat ccaaaaactt ttctaggttt aaattatacc atatttatag tatgatatga
      121 ttataatttt atataataaa taagtaaaaa tagtaaaaaa taaattaaga aatgttttta
      181 ataaaaataa gcttaagggg gtcaaccatg agtgcaaagg taacgaatca atatggaaat
      241 atagaaatag ataatcaagt aatagctcaa gtcgtatata gagctgcaat ggaaagttat
      301 ggcttagtag gtttagcata taagtctaaa ggaatagtag agctattaaa aggcgagaac
      361 gcaactaaag gtgtaagagt tgaggaagta gaagaagatg ccatagcaat cgaactttat
      421 gttataatac aatatggtac aaatatatct actgttgcca ataatataat agatagagta
      481 aaatatgtag ttgaaaaaat gactggcgta agagttacta gaattgatat taatgtacaa
      541 ggaataagag ttaagtaatt tcataggagg aaacaaatga ttcaatatat agatggtaaa
      601 aggttaaggg agatgtttat ttcaggtgca aacaatctcc aaaacaataa agagttagtt
      661 gacaagttaa atgtattccc tgtaccagat ggagatacag gaactaatat gtcattaaca
      721 atatcttatg ccttaaaaga attagcaaaa gttgaaaatg ataatatatc agatatcgga
      781 aaagcattat ctaaaggttc tttaatggga gctagaggaa actcaggagt tatactttca
      841 caaataatca gaggtatagc taaatctata gaaggaaaaa gtaaattatc aacagaagat
      901 ttagcaaaag cttttaaaaa tggttcagac acagcatata aagctgtaat aaaaccaatt
      961 gaaggaacta tacttactgt agttagagaa agtggagagt ttgcaataaa aactgcgaaa
     1021 aaagagaaag atgtagtaaa gtttttaagt atgcttgtga aggaatcaaa ttcttcatta
     1081 gaaagaactc cagaccttct aaagaatcta aaagaagctg gtgtggttga ctctggtgga
     1141 aagggtcttg tacttatata tgaaggaatg ttagcttcta taaaaggcga taacatagaa
     1201 ataaaaaatg ttgacctaga tactaatatt tcaacaagta tggattttgc aaagagtact
     1261 actagcacag atgatataaa atattgctat tgtacagaat ttattttaga aagttctaag
     1321 gtagaagata ctaaaataag agacataatg atggcttatg gtgatagcct agcagtagtt
     1381 ggagatgatg gagtcataaa ggtacatgta cacactaatg acccaggaaa tgtacttcaa
     1441 gaagctctaa agtacggtca attattaact ataaaaatag aaaatatgaa gttacaacat
     1501 gaaaatacat tactggatgt agaagaaaag aaagaaaatg atagtgaacc tttagaagaa
     1561 gaaaaagagt ttgggtttat agctacttct atgggtgaag gtttggcaaa tatatttaaa
     1621 gatttcggag ttgaccatat aatagaaggt ggtcaaacta tgaatccaag tactgaagat
     1681 tttatgaatg ctataaagca tataaatgct aaaaatatat ttattttccc aaataacagc
     1741 aatataataa tggctgctaa tcaggcaaaa gaattgagtg ataaaaatat aatagttata
     1801 cctacaaaaa atacgcctca aggatttgct gcattagtaa cttttaatgg agaattaagc
     1861 gaagatgaaa ataaagaagc tatgatgaat gctttaaact cagttaaatc aggtcaagta
     1921 acttttgctg ttagggatac agtgatgaat gaaattgatg ttaaagaagg taatataatt
     1981 ggaattgcag aaggaaattt attatcagca ggagattatg tggatgaagt aacatcaaac
     2041 cttattgaaa agttggttga tgaagatact gctataataa cattattctt tggtgaagat
     2101 gttacagaat ctcaagcaaa tgaactacgt acttctttag aagaaaagtt tgaagatgta
     2161 gatgtagagt tatattatgg aggacaacct ctttattatt acttaatttc agttgaataa
     2221 tgattataag aacctgtatg accaagtagc ttttataaaa ttggttgtac aggatttttg
     2281 tgtagtaggt gatatatttg ttggatttat ataaagatgt tcaatatgtt aagggaatag
     2341 gacctaaaaa agcagataaa ttaaataaac tgggtatatt tacactaaaa gacctattgt
     2401 attattttcc aagacaattt gaagatagaa ataatctaaa gaaaatagca caattggaag
     2461 atggtgagaa agttacgata aaagcagtaa tatcaagtat aaatacattt agcccaaaag
     2521 aaggtatgac tttaacaaag attgatgtaa aagatgagac aggctcggct aaattagtgt
     2581 tttttaataa gagttatata aaaaatactt ttcgtccagg agattcaata ttagtttttg
     2641 ggaaggttaa gaaaaaattt aataacttag aacttacatc atgtgaatta gaatatctta
     2701 ctaattcacc taagaataca tgtagattta tgcctgtgta ccagcttaca tacggtgtta
     2761 caaataaaga aattatgagt ataattagaa cagtacttga agataaagaa ttaattatac
     2821 aagaatatat gccacaaaga attatagaaa aatataggct atgtagtata gattttgcag
     2881 ttagaaacat acattcacca agtagtaagg aatcattaaa aatagcttta tatagaattg
     2941 tatttgaaga attacttata ctacaactag ggttgtttgt atttaaaagt ggcagaaata
     3001 aagaagatgg aataaagttt gaaacaagta aagacttaaa aaaaataata agtgctttac
     3061 catttaaact gactaaagct caaaacaggg cattagatga aattatacaa gatatgaatt
     3121 tagagaaaat aatgaataga ttggtacaag gtgatgtagg gagtggtaaa acagtagtag
     3181 ctctcttagc acttgcaaat tgtgttttaa atggttatca gggcgctttg atggcaccta
     3241 cagagatatt agcagggcaa cattatattt cacttactga gtctctaaaa gattttggaa
     3301 taaatgtagg tctattaata ggtagtcttg caaaaaaaca aaaggataca gtattggaac
     3361 aaataaagaa taatgagatt gatatattaa taggtacaca tgcactaatt gaagataagg
     3421 ttgaatttaa taatattggt ttagttataa cagatgaaca acatagattt ggtgttatgc
     3481 aaaggagcaa actttcattg aaaggagcaa atcctgatat attagtaatg acagcaacac
     3541 caataccaag aactttagca cttatactct atggagattt agatatttct attatagatg
     3601 aacttcctcc aggaagacag ccaatagaaa caattgctat tgaaaaaagt aagcgagata
     3661 gagcatataa taatcttgta agaagagagg ttgaatcagg aagacaagtg tacattgtat
     3721 gtcccttggt agaagaaagt gaagccatag aagccaaatc agcagttgaa cttgttgagg
     3781 agttaagagc ggaatatttt cacgacttaa ggcttgggtt gcttcatggt aaaatgaagt
     3841 caagtgaaaa agatgaagta atgagacttt ttaaagataa agaaatagat attttagttt
     3901 caacaacagt aatagaagtt ggtgtcaatg tgccaaatgc aacattaatg ataatagaaa
     3961 atgcagaaag atttggactt gcacaattac atcagcttag agggcgagtt ggaagaggta
     4021 gtcataaatc gtattgtgta ctaatttatg attctaaaac agatgtatgt agacaaagaa
     4081 tggctataat ggaagagacc aatgatggtt ttaagatttc agagaaggat ttagaaataa
     4141 gaggacctgg ggaatttttt gggacaaggc agcatggtct tccagaactt aaggtagcaa
     4201 acttatttaa gcatataaaa atactgaagt tagcacagca agaagctcga tatatattgg
     4261 gtgaagataa taatctacaa ttaaaagaaa acatggcact caaaaaagaa attatagata
     4321 aatttaaaga tacattgaaa gaaatttcat taaattaatt ttaaattgtg ttaaaatatt
     4381 actaatgtaa taatgtgctg aagggagatt gtcatattga gagtaatttc aggaaaagcg
     4441 agaggattaa aactaaatac tccaaaaaat gaagatgtaa ggccaactac agatagagta
     4501 aaagagtctc tatttaatat gataaattca tatataatgg aaagtgaagt tctggattta
     4561 tttgctggga ctggttcttt aggaatagag tgcttatcta ggggtgccaa gtcatgtact
     4621 tttgtagata tcagtaagga aagcatagat attgttaagt ccaatataaa aaaggcaaga
     4681 gttgaaagtg aaagtattat tttaaacctt gattttaaga ctgcaataga taaacttaaa
     4741 ttacaaaata gtaaatttga cataattttt atggaccctc cgtattataa aaatatgttt
     4801 atagaagcta ttgaaaaaat tgataattct aatctattga acgaagatgg aataatagtt
     4861 gtagaacatg atacgaatga tttatttcca gataagatat gtaaattgga gaaaactaga
     4921 gataagaaat atggaaatac tacattaact ttttataaga tggaggcata atatggaaaa
     4981 taaacctaga aaagctatat ttgcaggaag ttttgaccca attactaatg gacatttaga
     5041 tattatttgc agggcttcga agttatttga cgaattacaa ataggagtat taaataatcc
     5101 taataaaaag ggtctattta gctttgatga aagagtaaaa cttatagaaa aaagtacaag
     5161 ccatttaaat aatataaaag ttgtgacttt tgatggttta ttaataaatt actgtcagga
     5221 aaatgggatt ggagcattag ttagaggtgt gagaagtggt gctgatgttg attatgaact
     5281 tcagatggca catatgaata gagagcttaa tccagatata gagactataa ttttaccaag
     5341 ttgtacaaaa tattcattta taagttcatc tttaataaaa gaggttttgc tctttgatgc
     5401 tgatataaaa aatttggttc caaagattgt tttggaagaa ttaaagaaaa aaactagtgg
     5461 gggtaattga tatatgaaga tagatttaga aatgattgaa ctatttgagc agttagagga
     5521 gatatttaga agtgcttcta caatcccttt ctcacataaa tgcacagtgg ataaagatga
     5581 ggttttagca ataattaatg atataagaac tttaatacca gaagaagtaa cacaggcagt
     5641 atggattaat aaagaaagaa ataaaataat aagtcaagct aagcaagatg ctacaaatat
     5701 agttgaacaa gcgaaaaaag aagctgagag aatacaacaa gaatatcaag ataatataga
     5761 ggaattaaag aaaaattctg aagatgtagt aaaggcatat gtagaatcca gcgaacctgt
     5821 tgttcaagca gaccaaagag caaaagatat agttgataga gctgagagaa tagccaatga
     5881 gataagaatt gggtcaattg aatatgcaga ggatgtgctt tctagtgttg agtataattt
     5941 aagagaaata ttagaagaag taaaaagaga taaagctgaa cttagaccta aaaaataaat
     6001 tttaaataaa taaaaatgga gctatcacac aaaaaattcc aatttatgtg tgatagctct
     6061 cttttaatat agcaattttt tgttatataa ttgtgctttt tagattactt atatcttatt
     6121 ttgatattat aatgtagttt aatcttttat ttgttgaaat caacttattt atttgtataa
     6181 ataatatagg ttattttcaa atttttttaa taattttacc actgtcttct tttagattag
     6241 agttagactg tgcaaaatta taaaataata ctaaatcatg tgcaattatt tggtatggca
     6301 ctacatgtag aaaatataga taataaaatt aaggaggtag tatgaaggaa agggctaaaa
     6361 aaatattaga ttttattatt aaaaataaca atgtaacatc acaagaacta caagaaaagt
     6421 ttaatgtttc aaagagaact atttattacg atattctagc tataaatgaa caactaggaa
     6481 aaagtggtaa tataaaaaat gtaaaacaca aatttatatt tgaaggtaat ttatgtgatg
     6541 caaggaaaat aatttcaact gaagaagata aatttttaga tagtgattac agaaaaactt
     6601 ttatattaaa caagatatta cttggcgaaa aaatatcaat agaaaaacta acaaatgaaa
     6661 tgttactttc aaaaaacaca gtagtacaaa caataactga agtaaaaaaa taccttcaaa
     6721 caatggggtt aagattagag tataagggaa agtacaaaat tataggtgat gaatacgtta
     6781 ttagagagct gtttctgata attgttcaag aaaatgtact ggaaattaat tctataagtg
     6841 aggaagtaag tagtttcgat acaaaaggac atataaaact tactgattat tctttattaa
     6901 atctaactaa gtttgtggaa tttctaaata aaaggataag agatgggaaa acattatact
     6961 catataaata tctaaatgaa gctaaaaaaa ttagttactt tagtaattgt aaagaacttt
     7021 tatgtgaaga agctaatgaa aatgaacaag cctatatatg cacttacata agttcacttc
     7081 ccagcttaaa taatgaagtt aaggaagatg ttgttgaaga atatatagat aaacttatag
     7141 ataagtttga agtaaataca gcaataaaat tagaaagtaa gcatgagttt aagaaaaata
     7201 ttttaaggca cctccactct tcttacaaca gaatacgatt taagtttcct attagaaatc
     7261 caatgttaga tgagactaag tataaacatg agtcacttta taaaattata aaatcaatta
     7321 tagagaatga agaagagttt cctgtatttg aggggataag agaagaagaa ataggattta
     7381 tagcagcgta ttttggagga tatttaagag ggtctaggga taatggtcta agaagaaata
     7441 aagtcttact agtttgtcct aatggtctta tggtatcaaa gagtttggaa atacaactat
     7501 ataaatacat accaaccata gaaatagtag gtatagtatc cattaagcaa ctaaaagaag
     7561 taaatgtata ctacgattac ataattacaa ctatagatat acaaaatgta aacaacgtaa
     7621 ttgttgtaaa tcctctttta acatcatcag atgttcaatt acttatgaac aaactaataa
     7681 gtgtaaaaga aaacgagaag tatttcaatc ttgaattaat aatacaagct ataagaaaaa
     7741 atggagttat caataatgaa gaagcactaa aagctgattt attaaacata atacacaaga
     7801 tagatgaagg agagatgtat caaccaatgt taaaggaatt aattaatgca gaaagggtca
     7861 atataataaa aaatgttaga gattggaaag aagcaataaa aatagcctca aagccattgt
     7921 tagaagataa ctctatagaa gagttatata tagaaaatat gataaagtca gttgagaaat
     7981 atggaccata tattgtttta gctgatagat ttgctctacc acatgcaagt tctaaagaag
     8041 gagtaaacaa gcttgctatg tctttactaa tagtagaaga tgaagttgat ttattaggaa
     8101 aaccagtaaa tatatttatg gtattagctg ctgtagataa cactactcac attagagcat
     8161 tagcatcact atcagagatg atgtatgagg aagaaaatgt aaaactaata attaatggtg
     8221 ataaaagttc aatactagaa cttataaata agcaaaatta ggaggaatga aaaatgaaga
     8281 tattagcagt atgtggtttt ggtgtaggtt catcaatggt attaaaaatg acaatccaaa
     8341 aggctctaaa gcagttaggt atagaatgtg aagtagaaaa tacagatata aattcagcta
     8401 gaggtgctga ttgtgactgt atattcacaa gtcaagaatt gttaaatgaa ttaaaaggaa
     8461 cttgtaatgt agatgtatat ccagttaaaa agtacatgga tttagcagaa gtcacagaag
     8521 ttgttcaaac ttttttaagt gataaaaatg ataagaaata gtataagttt taaaaagtaa
     8581 atttaagtta atcagaataa aataaaaact tactaaattc aaaaatagta agaaagagag
     8641 gagggattta gtggtagaaa acttaaacag ttgacctatc actaaaaaat ataatgcttg
     8701 attttatttc aaataatatt ttacgtaatc catctatgtt aattggatta atagcagcat
     8761 ttggtcttgt attacagaag aagaaagcat ctgatgttat aaaggggtca ctacttgcag
     8821 ctttcggaat tataattatg gagactggaa caggtatgtt agttagttca atagcaccaa
     8881 ttaatggagc tttccaaagt ataagtggtg gtgcagtaac aaaaggttta agtgatgtta
     8941 cttttactgc aagttatggt ggaattatag gtttagcaat gtttgcgggt ttagctattc
     9001 acctaatgat agcaagattt actcctatta agacaatctt cttaacaggt cacatgcttt
     9061 attggttccc attcatattt gtagcttcag gagttgaagc tggattaaat ggtggagtat
     9121 taatagcttt tgcagcagta ttatcagcac tctattggtc aataatgcct tggttattaa
     9181 gaaaatatgt gtttgatgtt ataggagatg attcttttac attaggtcat ccatctggtt
     9241 gtttagcact tatatcagga tttgttgcta aattagtagg aaataaagaa aaatctacag
     9301 aagatataaa catacctcaa agtttatcat tttttagaga aatatctata agtggtggaa
     9361 tggcagtgtt tttaatgttt atagttgttg ggatagtaat accaggtgcg tttaaagata
     9421 gtaatgagtt tattggaacg tatgcaataa agcaaggtct ttcttttgga gcaggtctta
     9481 taataatgct tcatggggta agaatgctaa taaatcaaat aatgccagca tttcaaggta
     9541 tatctgaaaa agttgtgcca aatgcactac ctgcttttga ctgtccaata ttatttaatt
     9601 ataaaccaaa tgctgtatta ataggattct tagttgctat gctaacatct acagctatga
     9661 ttttaatatg taactacttt aatgtattta atttgctact tataccactt gttataactt
     9721 cattctttga gtgtggagca gcagcagtaa taggagaatc acaaggtggt atacgtggag
     9781 ctataatagg tacagctata gctgcaatat ttatggtagt tttagtagga atatctgctg
     9841 taatttactc aggaactatt caaaattgga tactaatatt tggaggtaat gacctttcat
     9901 tatttggtat aatcagtagg tttataggtt cactatttgg aggaatatta tagatatgaa
     9961 atttaaatac atagatgaga taagaagatt aattgatata atagaaaacg aagaaaaagt
    10021 caatatggaa aaagcagtac aaatctttgt tgaagcaata gaaagtaaaa atgctatatt
    10081 ttcatttgga gcttcacatg caggaatatt aacagaagaa ttattctata gagcaggtgg
    10141 ctgtgttctt ataaatccag tatttgcaaa atctctaatg ttagatacag cgcctataac
    10201 tcacacaagt aagatggaaa gattagaagg atatggtaca gctctagctg ataaaactcc
    10261 aattaaaaaa ggagatgtag ttatagttca ttcagtatct ggtagaaatc cagtaagtat
    10321 agagtttgct atgaaagcta aggaaaataa ggcaatagta atatgtataa ctaatttaag
    10381 ttactcaaaa agtgtatctt caagacattc ttcaggaaaa aaactttatg aagtatgcga
    10441 tttagtgata gataatcatg gtcaaaaggg agatgcctgt gtagagatag atggattaaa
    10501 ccaaaaagtt agtccaactt ctactgtaat agcaactact gtgatgaact ctattatagc
    10561 tcaagctact caggaattag ttaataaagg gctaaagaat cctccgattt tctatagtgc
    10621 aaatattgat ggtggagatg agttaaataa gaagattttt gatgaatata aaagtgtaat
    10681 tcattatgaa tattaaggtt aaaaaaatat gttaatgaac tttgaagttt atcaacaaag
    10741 aatattaata atatataata aatgctatca tttaaaagct gtaggtaaaa atctacagct
    10801 ttttattatt gtagattaaa aagatgcgta attaaatttt aaattggaga aaataaattg
    10861 gaaggtatca attataaata ttagaaacgt tgtcatagaa aaaaactata ttaaaaaaaa
    10921 gtacatagaa aaaactgatt agacaataaa tacatgcttt gataatattt taaatggagt
    10981 gaaaatgtgt agaattataa tgaaaaaaga aaataaaaat gaaaaaagaa aataaaatgg
    11041 agggaataaa agtgggtatg ttaaaaaaat tatgttcact agctatggta gcaattatga
    11101 ctacaacact tataacaggg tgttcaagtg gaaatggaaa agattcaaaa aaggatggcg
    11161 aaaaagaaaa actagttctt tggatgccac cagtagaaaa taatatgaag gaagtttggg
    11221 accctatttt agataaattt gaggaaaaaa ataactgtga agtagattta cagataattc
    11281 cttgggataa ttattctgaa aaatttgcaa cagctataag tgctggcgaa ggtccagatg
    11341 taggatatat gtatgcagaa atgtatccac agtttataga aagtggagca gtagaagatt
    11401 tgactaacta tgcaactaaa gaggataaag agcaatctat ttatataaaa acatctgaaa
    11461 tgatgggtgg aatgtatggt atgccattcc aagcagcaaa tccaggggta ttatactata
    11521 acaaggatat tttagagaaa ctaggagaaa aacctcctaa aacatgggaa gattttaaaa
    11581 gaatttgtaa aaaagcaacg caagatactg atggtgatgg aaagatagac caatggggat
    11641 tagctcaggg atggggagct aaaacttttg gtaacttaaa ctggaactgg tatccttatt
    11701 tatggcaagc tggtggagat atatttaatg atgatttaaa atcggttaaa tttaatgata
    11761 agagtggttt agaagcagct aagttcttaa aagaattaca agcatatgtg cctgaagatt
    11821 ctttatctaa agatagtaat gaaatgatag aaagtgtatt tggaccaggg aaagcagcat
    11881 ttacaataat gttatcttct gctgcaacaa gtgtatttga tgaatcattc ccggatttaa
    11941 attggggatt tgtaactgga ctagaagata agaaagcagc aacttttgga gcagtagacc
    12001 atttatcttt aatgtcatct gcgaaaaata aagaacttgc atacaagtta atacaacata
    12061 tgttaagtgt tgaatcaatg acagaatttc ataaagcaat accaagagca ccaataacaa
    12121 aaggtgaacc atatcaaggt gatgaaagat tcaaagaaat ggtggaaaat gacaaaaatg
    12181 tatatagacc attagtagta ggacctcatg gagttgaaat atatgaatat ttatggaaag
    12241 aacttcaaac tatgatttca ggagataaaa ctccaaaaca agcgttagat gatgctgcaa
    12301 aatattcaaa tgatttatta gcacaataaa agtttaaagc tgtaatatgt tgtaaaggac
    12361 tttacagatt aaaattagca ttgtatttat aaatacaatg ctaatattta tattggagag
    12421 gataaaatgg aaaacttagt tagagagttt gaagaaaatt caaaaagaaa aaaaacaaaa
    12481 ttaaatatta aaccatatat gtatatattg ccattgggaa taattttagt ggcgttttat
    12541 gtgataccaa taataatgtc aatatacttt agttttacaa agtataatat tattagtcca
    12601 gctactttta taggtttaga aaattataaa aaattattta cagatgaaac attaaaagta
    12661 tctattataa atactattaa atttactgtt gttgtagtac cttgccaaac tatattatca
    12721 ttgattttgg ctgtttggat tacaggaaaa ggaaatagta aaatagcttc atttgctaaa
    12781 ggtgctatat ttataccagt attatcatct atggttttga taggtatggt atggagggct
    12841 ttattgaatg gagaaggttc tattatatat caggtattag gtgcctttgg aatagaatca
    12901 tctaaacttt taggagatag taatacagca ttacctacac ttatgtttat atctatgtgg
    12961 aaaaatatag ggtatttcat ggtaatttat atttcagcta taatgaattt accaaaacac
    13021 tgctatgaag tagcgaaggt tgatggggct acaaaacttc aagaatttat aaaaataact
    13081 gtacctttat taaaaccaac tacaataatg gttgtatttt tagggtcgat atggtcatta
    13141 caagtatttg acttaattta tactgtgaca ggtggaggtc caggtatatc tactatgagt
    13201 atagttatgc atgcatttaa cttaaacttt aaaaacttta attctggata tgcaatgact
    13261 gtagcaaacg tattattttt gttaatagca gtagtttcga ttttacaaaa taagcttgta
    13321 aaacgagaca attcagattt ttagaaaggt aggtataata tgaaaacagc aagaagatta
    13381 ttaagtgatg gtttactttt atttatagca tgtgcaagtt tggtaccttt tatatacatg
    13441 ttgataatat cattgaagat aacatataac tcatatagtt tggatatatc tttttcaaca
    13501 gtaacccttc aaaactatat agatattttt actaaaaaag gatttgcaca atattttttt
    13561 aatacagcaa tagtatcttt ttcaggtgta ttattaaatt tagtatttag tacattagca
    13621 ggatattctt ttgcgaagat ggattttaaa ggtagtgata agttattttt atttatgata
    13681 atgactttaa ttataccttc ccaagttaca atgataccac tatatataat aatgaaacac
    13741 ttaggatgga taaatagtta tcttgcactt attatgccaa taccaactgc atttggcgta
    13801 tttataatga gacaagctat tttaggagta ccaaaagagt tattagaatc agcaaagata
    13861 gatggttgct cggattttag aattttaata caaatagtgt tgcctttaat taaaccagca
    13921 ttaattacac tagcaatatt tacttttatg ggagcatgga acgagtttat gtggcctctt
    13981 atagctacaa ctaaagatgc gatgagaaca ttaacagttg gattatctac tcttaagaca
    14041 ttccagataa ctaattatgg acagatgatg gcaggggcta caataacatt tttaccgcca
    14101 tttattttct atttgatttt acagagtaaa tttgttgaag gtgtgtcttt gtctggtata
    14161 aaaggttaaa tagtctaatt taataatata taaagaaact tatataaata tggaggaatt
    14221 taaactatgg aaatttcata tgtactgaaa gattgcacat tcaaacacaa taaatattca
    14281 gttatagaag ctgaaacttg gaaagataag gatttggata ttgtagtagc taaaggtgaa
    14341 aaatttgcat ttcaaattat gttaaaagca acagaagaat ttaattgtac aattgataaa
    14401 agtagtgcta tatcatggaa aggcctggga aatagagtaa ggttagcatt aaatatgcct
    14461 aatagtttag aaaataattt tagtataaat atattaggat atgttcaaga tgatacaaag
    14521 gcatttgtga atgatgctat tttaagggat aaagatgtgt tagtagagca atatttgcct
    14581 caaacatttt ggattgaagg tcaagtacca gaagattttg aagaaaataa ccttgatata
    14641 ggtattgata tatttaaaag ctttggatat gaagatgaag aaaaagtttg tacaattgat
    14701 gttcctgtaa aagtaagaaa tgttgtgcta aagcctttag atgaaagcaa gttcttttta
    14761 gatttatggc aacatccaag ttgtttagct agaatgtata aagttgagct atggtctgat
    14821 atacattttg aaatagtcga taactattta aaagaattag catctttagg agaaaaagtt
    14881 gcaacagtta tagtttcaga ttatccatgg gcagggcaaa gttgttataa agtttataaa
    14941 aatccatcaa acttatatga atataacatg gtaagtgtat caaaagaatt ggatggaaag
    15001 attaaatgta actttgagag tatggatagg tatataagca tagctgataa gtataaaatg
    15061 gctaaggaga tagatttatt tggtctatta ggtaattggt gtgctggtga gtttggaaat
    15121 cctgtagaag gttataagga ccctataaga gtaagatatt ttgatgaaaa ggataaagta
    15181 tttaaattta taaacaatac aaatgattta aaagaatata taggattagt attaaatcat
    15241 ttaatagagt gtggattatg ggacagagta agaattatag ctgatgaacc aaataatcca
    15301 gaagtagtca aagagtgtat agaatttata aattctactg taagtactca tcaagttaaa
    15361 tataaatcag caactcacga ccaaaacttt ttagacagag ctaaagatga aattgatgat
    15421 atgtcaataa atttaaaact tactatacaa aattataaag atatagaaag tcttaagaaa
    15481 aaaatcaatg acaagggtgg gatattgact tggtttgtat gctgttttcc agaaaaacca
    15541 aatagttttt taagttctcc ttttgtagaa aatagaataa taggatggta tacttattac
    15601 tttggtcttg atggattttt aagatgggac tataatttat ggacagaaga cccttggaaa
    15661 gattcaagtt acaaattccc tatttggaaa gctggagata tgttctttgt ttatcctgga
    15721 aaagatttaa agccagttag aagtgtcaga atggagaatt taagatttgg aatacaggat
    15781 tttgagttat ttacaatgtt agaaaaagaa aagggaagag agtatataga agttgagctt
    15841 atgcaagaat tattaaataa aaaagaaaat gctgaaatta aaggttttgg tgacatagaa
    15901 ctaggatatt cacttgataa ttggagatat gataaagtta aaaaatatgt attagagttg
    15961 ttagacagag tataaaaaat ataagagtgt ctcaaaatga actttttagt tcatgaggca
    16021 ctctttttgt atgaaatcaa tatagtttca ttgtttcaac aatgaaaaat aggattattt
    16081 ctattctaag acagcccttt agttttttag ttgtattcaa tgttttaaaa ctaactataa
    16141 acgctataaa gtttataaat gacaataatt atatataaca tgatgtactt taatttgcta
    16201 taatagggat tgaataatat tatatggaag ataacattgt ttggggggat taatataaaa
    16261 agaataggta ttattataat gtttttttta agtatatctt ttattggatt ttatcttgta
    16321 ataagagata acagtgatac agttaagatg caacgaggga taaatatagg taatgcacta
    16381 gaatcaccta aggattttcc gtgggatgta aaaatgtcaa ataagttttt tgatgatata
    16441 aaagatgcag gttttgacac agtacgtata ccagtaaggt tttctgatta tacgtcagat
    16501 tctgataatt ttaaaattga tgaagaattt tttaaaaaga ttgataaata tgtagattat
    16561 gcattggata aagacttaat agttgtgttg gatttacacc attttgaaga aattatgaaa
    16621 gaaccaagag ttcataaaga aaagttttta aaaatatggc aacagatagc taataggtat
    16681 caaaaatatg ataaaaaatt ggtatttgaa ttattaaatg aacctaaaga aaatttatat
    16741 tcacagttac taaacgaata cattgaagaa gcaataaaga taattagaaa aactaaccct
    16801 aaaagaacta ttatagtagg accatataat ttctatcaaa ttgattattt gaatgaatta
    16861 aatattccta aagattctaa tatagtagtt tcctttcact attacgagcc aaatgatttt
    16921 gcatttcaag gaaatatcta tcataaggga tttgagcact taagcaatat tacttgggaa
    16981 ggaacaaatg aacaaatgga ttatttaaaa aaaaggtttg atactgtgga aaattgggca
    17041 aataaaaata aggtaaaaat cttcttgggt gaatttggag taacaaaaga agcaccagaa
    17101 acttcaagga gagcttgggt aaaagctgta agagaagaag ctgaaaaacg aaacttttca
    17161 tgggcttatt gggagcttgc ttctggattt ggtatataca atcaaattga agggacttgg
    17221 gatagagaaa tattaagtgc attaatagaa aagaggtgat tttatgaaga aaaattcaaa
    17281 aaagatactt ataactttaa ctattattac taatatagta tatatattgt ggagaatttt
    17341 ttatacagta cctaaagaag aagggatgtt tgccctaatc tgtgccataa tacttctttt
    17401 tgtagagata atgggcatga tggaaatgtt tgtgcattat tatggtatgt caaatattga
    17461 gtaccctgaa aaaccaatca taagtgaaga actttatcca catgtagatg tattcattgc
    17521 aacatataat gaatctgttg atttggtgag aaaaactgta aatgggtgta ttcatatgca
    17581 gtatccagat aagaaaaagg tacatatata catatgtgat gatggaaatc gtgaagaaat
    17641 gtgtattcta gcagaaaaaa tgggtgtaaa ttacataact agaactgaaa gagaaggagc
    17701 aaaagctgga aatttaaata atgcaatgca acatacaaat tcccctttga ttgctacatt
    17761 tgatgcagat atgataccaa tgcatgactt tttaattgct acagtaccat actttttgaa
    17821 aaatgagcaa gctaaaaaag atggagaaaa agaggagtat gaaaaggttg gttttgtgca
    17881 aactccacag agtttttata atccagactt gtttcaattt aatctgcact ctgagcgaag
    17941 gattccaaat gagcaagatt atttttacag agacattcaa ttggctagaa ataggacaaa
    18001 ttctgtaatt tatggtggta gtaatacagt tatttcaagg gaagcacttg aagaagttga
    18061 tggattttat acttattcta ttacagaaga ttttgcaaca ggtatcttaa ttcaaagtaa
    18121 ggggtataga tgttatgcta ttccagaagt acatgcatca ggtttatctc cgacagattt
    18181 aaaaagtctt attaagcaac gtgaaagatg ggcacgtgga tgtatacaaa ctggtagacg
    18241 tctaaatatt ttgtttagga gaggtttagg tttttggcag aaaataagtt atatttcttc
    18301 aattacttat tggtatgcaa gtataaaacg ttttgtatat ataatggcac ctattttatt
    18361 ttctgtgttt aatgtgattg tagtaaaatg tacattactt caagtattgg tattttggtt
    18421 gccaatgtat gttttaagta gtttatctct taaaatattt tctcaaaata taaggaatgt
    18481 tagatggacc aatatatatg aaactataat gtttcagtcc ttaatgccag cagtaatact
    18541 tgaaacgttt gcaatatcta aaaataaatt ttcagtaaca aataagagta aattagaaga
    18601 aaatagaatg tataagtttt tacagggaat accttatttt atttacatgg tcttatctat
    18661 cataggaatt ttaaaaatgt ttgtagcaat atttaaaatg agttccataa catattcagt
    18721 tgtattattt tggttaattg gaaacctttt taaccttgtt atggctacat tatttatatc
    18781 agggagacag cagctaagaa agtctgagcg ttatattgct gaaattgatt ttaagttaaa
    18841 gcaaaattct tatgttcttt cttcaaaaac aattgatata tcagaaaatg ggtttgcgtt
    18901 tttgctagaa aatccagaat atatttcccc agaagaagag tttgaggtag agtttagaga
    18961 aaaatctgga aatgaaatgt atatagctaa tatgaaagca aagattgtta atgttgtgga
    19021 agtaaattca aagtggaaat atgcagctta tattacacat attgaagata gtgaaataga
    19081 taattggatg tgtattgttc acgatagaat acctacttta ccaatgacaa tatctaatca
    19141 actaggtttc tttgatgatt tgcaaattaa tgtaaaaaaa cgtattgaaa aaactagaac
    19201 attatcacga agaagtccaa gaatcaatat gaattttcat atgtatataa aaaatatagg
    19261 taaattaaga atagtaaatt ttaattatca atatgtatta ttaaattttg agaataaaaa
    19321 tatttatcct aaagaaatag cattggaaat tagtgaaggt attgtactag aatgcgattt
    19381 atgtgagggt aaaattgatg aaagaggtat cttatataga gtaaataaca ttgacagcat
    19441 aatgcaaaat ttatttttaa gagatgaaat gatggattgg atattacaaa ataagaaaat
    19501 tcttgattca aaacctaatg aaaagaaaga aaaaagtata gatgagtttg aacccatgga
    19561 atatatttag gagagatgaa atgaagactt taaaagtgtt tttttgttct gcaaaatatt
    19621 ttattttagt gtttattttt tttataaata ctacttttat aagttcagca acaagtgata
    19681 aaattttaga tttgtccttt aaaaagatag agactgactt aagttctaag attacatatg
    19741 aagatacagg tgtaaaaata gaaacgaaca gttctaaaag tgataagaaa cgttatttat
    19801 atatttacca aaatatgaaa gaaaattgga gtatgtataa caatttttat attgaaattc
    19861 aaaataaaaa taaaagttct cagaaaataa atttaagcat acaaagtaaa aatatgcttg
    19921 aatttagatt aaaagaaggt agtgaagtat ttttagaaga taaaaatata atatattcag
    19981 ataaaataaa agaaggatgc atagaagtac caggtgaatt tgaaggcaaa atttatgtga
    20041 actttaatag ccttataaat gagaaaagta atgttgtgct agactcaagt atgctttcaa
    20101 acattgttag ttggggaatt acatttattc catctgatga agaacataat aatattatta
    20161 taaaaaagat atctttattg tctgaagaga aattaagttt tttacataat attaaaataa
    20221 ctggcaatga agaagtacaa ataccagtat tgggtcaagg tataagtcag tatgaggtat
    20281 taggtcttga aagtgattct aaaattaagt attctcttat aggaaaaaaa gataatttaa
    20341 gtatttctca aaaaggtaaa ttaacattaa ataataaatc caaacctgga caaattattt
    20401 tacaagttga cgttgatgat aagtttaaaa ttggaaagaa aataacatta actgagtctt
    20461 ggagtgttaa taaaaaagat aaggatggtg ttccatatac attagtatat cctgagcaat
    20521 caccaacagt acaagatatg aaaaaaataa actttatgaa taacatcata acgtttgttc
    20581 gaattttatt tgtatctcta gtaataatat gctttggaat atatttatat tggaaaaaat
    20641 gttcaaaaac taagtaaaga gaggaggtta gattgtgtta ttttcaagtt taattttttt
    20701 attttatttt ttacctataa ctttggtttt atattatgta tttaggttta accgaacgat
    20761 acagaacatg atattgcttg ctgttagttt atttttttat gcttggggag agccaaaatt
    20821 tgttgttatc atgatagttt ccataataat gaactatata tttggtttac tggtagatag
    20881 atatagggaa agtaaaataa aagtaaaagt ttttttagtt ttgatgtgtg catacaatat
    20941 aggtgtttta tttatattta aatatctagc ttttgcgcta agaaatatta gtgaacttat
    21001 tagtactgaa ttgactatac ctagtattgt tttacccata ggaatttcat tttttacatt
    21061 tcagggaatg tcttatgtta tagatgtata tcgtagacat ggagaagtcc aaaagaaccc
    21121 tttttatgtg ggattgtata tagcattttt cccacaatta atagcaggtc ctatagttag
    21181 atatgagagt gtggcagaac aaattctcaa tagaaaagaa acttggaata aattttctat
    21241 tgggacttgt aggtttataa caggtctagg taaaaaagta ttaatttcaa ataacatggc
    21301 tatagtagca gactatattt acactatgaa tagtcaagga gaaatagctg catcacttgc
    21361 atggcttggt tcaatagcct atactctgca aatattcttt gatttttctg cttattcaga
    21421 tatggcgata ggcttaggtc ttatgtttgg ttttaaattt gaagagaact ttaattatcc
    21481 atatatatca aaatcaatta gtgagttttg gagaagatgg cacatttctt tgggaatgtg
    21541 gtttaagagc tatatatact ttccgcttgg aggttctaga gtagcaaata aagatattat
    21601 gattagaaat atgtttatag tgtggttatt cactggaata tggcatggag cagaatggac
    21661 atttgtaatt tgggggattt taaactttgt tttcctaatt atagagcgat ttataatgtt
    21721 tgaaaagatt gaaaatcaca attttataaa acatatttat gctttattag tagttaattt
    21781 tggatgggta ttgtttaggt cacctaattt aaaagaagca tataattatt ttaaagctat
    21841 gtttggaggc aatggagcta tatggagtga ttatacatat atgtttttaa aagaatactg
    21901 ggttttcttt atatttgcat ttatttttag tgtacctatt gcaaagaaaa tcaataagtt
    21961 tgttgttgaa caagctaaat atagtatgat atttaacgta ttttatccaa tctcagctat
    22021 tttattgttt tttatttctg tgacatattt agtaactgga agctataatc catttattta
    22081 ttttaatttt tagttgggag gaaaagtatg aatagatttt ttaaagtaag attaataact
    22141 gcaattatgt tcttaattat tttattcgct ttctctatct ttaatgtttg ttattctgga
    22201 aaagatacga tacaggaaat aaaaaattca atagctgaaa aaagtagttt aaaagataca
    22261 atatcatcta ttgataatac agtgaatgac aaagtaatat acaaaaatac gttcattgaa
    22321 acttatggct atttacaaaa attaatgggt aaaaatgagt tttcaaaatt tagtgttgta
    22381 aaagatactt ctggaaagat gcattataca tattttgcta aagctccaaa tccagttggt
    22441 gtgatagcag atagggtaag aaaattttct gatgaaatgg aaaagcaaaa cactaaattg
    22501 gtttacctaa tgactccaga taagtatata aaaggagtga caaaatttcc aaaaggaata
    22561 ccttattctt atagtaatga gacagcagat aattttttaa atcaactaaa aaagtataat
    22621 gtggactata ttgattttag agaaaatatt ttaaagagtg gtattccaaa agatgattta
    22681 ttttttaaaa cagaccatca ctggaaagta gaaacttctt tttgggcatt tggagaatta
    22741 gttgagcaac ttaataaaaa atacaatatg aatttagatg aaaatcatta ttatagagat
    22801 aaggacaatt ataattcaat tgtatatcct aagtcatttc ttggttctat gggaagaaag
    22861 actggtatct tatatggtgg tatagatgat tttacattaa tttatcctaa atttaagact
    22921 aattatactt attatacaga ttctaaatct caaaaatttg aattaaaagg aaggtttgaa
    22981 gagagtttaa tattatcata tccttttaat gcagaccttg atttaatgga tggtcaatca
    23041 gataagtatt ttacatattt attggggaac agaccattag ttaaaataaa aaatatagaa
    23101 aatccagatg gattaaaagt gctgtttgtt aaagattctc taattgtacc aacagcagca
    23161 tttttttcct ctgtttgttc atctatagat atgatagacc ctagatatta tgatggagat
    23221 atactagaat atgcaaaatc ccatgactat gactttgttt ttatatctgt atatccccaa
    23281 aatcttacaa aagaattttt tccgttttgt gagtaaattt ctaaaaaagt tttctgataa
    23341 aataaatggc taactggtaa aaagttagcc atttattttg agtgttattc taagaaatat
    23401 tttttttact aaatacttct ttatctattt ttccaagtga ttttcctaca gtaattgatg
    23461 ataccatagc accattaaca tttatcatgg tacgtcccat atctagtata gggtctattg
    23521 caagtatacc accaactaat ggaaaataag aaccaagacc aactccagag ataacaactg
    23581 aaacggccat tgtagcagtt cctggaagcc cagctattcc aaacgaacta attgttataa
    23641 caattaacaa cataacataa aagctcatat ccatgtttac tccagccata ttggcaagtg
    23701 ttacagccat taaagcagga tatatacctg cacaaccatt catacccata tttgcaccta
    23761 aacttgctgt aaagcttgat acaccctcat caactccgtg attttgtact agactttcta
    23821 tcgttacagg caatgttcct aaactagaac gtgaagtaaa tgccagaagt aaagcttcag
    23881 ttgaattttt tatataagta atgggattta gaccatttaa agttataaaa attaaatgta
    23941 taataaataa tattgcaact gatatataaa gagctaatat aaactgtaaa acagatacta
    24001 ttgaactaat accttgtgaa gttattgtat ttgcaagcag agcaaccaca gcataaggca
    24061 taaatttaat aacagtcata gacacactta caattatttt gtaagatgct tctataaaat
    24121 ctataaatgg ttttattgtc tcaaaatgtt ttttacttaa tctctttatt gaggtagcta
    24181 taaatgtagc aaatattatt gtagctacaa tatttccatt tgccatagcc attacaggat
    24241 tagatggtag taatcctctt aaagtatcca caagagagtt tatttcttta agttcttgat
    24301 tttcaactaa agtaggcata ttagctccta cacctaactt cattatattt gcaacgataa
    24361 ttccaacaat tgcagctata gcagtagtag caagaagcat acctaaagat ttaaatgtca
    24421 gagttccaag attgttatct ttcatattta taattacttt tataatggat aagaaaacta
    24481 aaggtactac taacattttt aataagtcca taaatccaga accaaatagg ctataccact
    24541 tacttacttc ttgtagccaa gtgacttgag ttgtatcatt aggaaaacca gcaaaagctt
    24601 gtattatgat tcccaatatg agtcctagta ctgtagcata tattgttctc aatgaaaatt
    24661 ttattttttt cttttcaaat tgtctaacaa taaagaacgt acctagaagt aatattatga
    24721 aaaaaattgt tttaaaatct gtaatcataa gaaattgtga taaaaatgaa ctttccaata
    24781 tttttctcct tttatttaaa aataaatttt tctaagttta aatgatatca atatattaaa
    24841 aaaatgtcaa tattatatca caatttactt aaatagagta aaacaagttg attttgaaaa
    24901 atgtgattaa gtacagaaaa atagggtata ataaatgttt aaagaatttg aattgtggta
    24961 agaaaataaa aaatagcttt tataagattt gatttcaaat taaaatatgt taaataaata
    25021 actaaaaatt attataaaaa atgttttcat agaatatata tcaaatgaaa agcttaaaat
    25081 aagtgaagaa attaactaga catcaaagga gagataaaat gagagtaata ataattggtg
    25141 gtgttgcagc tggtatgagt gctgcagcaa aattaaaaag aataaaacct gagtatgaag
    25201 ttgttgttta tgaaaaaact gaaatagtat cttttggtgc ttgtggatta ccatactttg
    25261 ttggtggatt ttttaatgat gctgatgaat tattagcaag aacaccagag aaacttagag
    25321 aagctggaat tgatttaaat atatttagag aagtcgttga ggttgatagt gaatctaaga
    25381 aaataaaagt aaaaaacata aaaacagatg aaatatatga agattactat gataaattga
    25441 tgatagcaac tggagcaaga agtattatgc ctcctataaa aaacataaaa ttgaagaatg
    25501 tttctacatt aaaaagttta tatgatggag aatatctaaa aaaactttta agtaatgaag
    25561 ataataaaag agtgacaata ataggagctg gttttatagg gttagaagca gttgaggcat
    25621 gtaagaaatt aggtaaagat gtagatgtaa ttcagttaga agatagaata ttgccacaag
    25681 tatttgataa ggagatgacg gatgtattag aagaagaaat aaatagacat aatgttaatt
    25741 tgcacttaga tgaaatggtt gtagaacttt gtggagaaga taaagtagaa aaagttataa
    25801 caaacaaagg ggaaatagat acagatgtag taataattgc tactggcgtg agacctaata
    25861 ccgagttttt gtcaaatact aatataaaaa tgctaaaaaa tggagctata gtagttgatg
    25921 agtatggaag aacttctgta gaagatatat actctgctgg agattgtgca actataaaga
    25981 atatagtaag taatgaaaat gtatatgttc ctttagctac tggagctaat aaattgggta
    26041 ggatagtagg agaaaaccta gcaggaagag aagtatctta tcaaggttca ctatcttcaa
    26101 gctgtataaa aattatggat atggaggcag catcaactgg aattacagaa aagcaagcta
    26161 aagatttagg cataaatgtc aaatctaaat ttatttctga ttataatcaa actcattatt
    26221 atccaggaag aaataaaata tatgtaaagc ttatttatga tgcagataca aaagttattt
    26281 taggcggaca agtggctggg tttaaggatg ctgtccaaag agcaaatgta ttggcagctg
    26341 ctatctttgg aaaaatgacc acaaatcaac ttggaatgtt agatttatgt tatgctccac
    26401 catttgcaag aacatgggat gttttaaatg tagctggaaa tgtatctaaa taatatttag
    26461 attatagcta atatagttga caagcaatta agggaatgaa tttacctaaa ctatttttat
    26521 catggtaaaa ttcattccta aatttttatt ccaaattttt agaaataagg attccattaa
    26581 attattcatc aaaatttaaa cgcaagctta atctatcttc tccaagaata gtattttcaa
    26641 aaacattagt agcattttct aaatacatac taggtatgtc taaagaagga ctaaaatgag
    26701 tcagtaaaag ttgtttgaca tttccaagtt ttgcaagatt agcagcttct ctaaaagtca
    26761 tatgtttgtt tctaacagct tttgatatat ctaaatcatc accatacatg gcttcacata
    26821 taaataaatc actatctttt atgaaatctg gtattgattc tataggtctt gtatcggttg
    26881 taagacttat cttaactccc tttctcaatt ctcctaagac catatctgga gtgtattgtt
    26941 ttgaatctaa aactatattt tgaccttctt ggagtttgtt ccataatatt ttaggaactt
    27001 cgtttttagt tgccttatct atatcaaatt tagggtttct tctaaaatta aaactgtatc
    27061 ctaaacactc tatagagtgt tctaaactga ttgttgaaat ttctaaattt cttaaaactt
    27121 tattatctaa agaaaaattg ccttgagggt tttctataat ttttaaagtg tatggtacat
    27181 attctactaa atttctcata gaacgtatac aatcgactat tccaactgga ccaactatag
    27241 ttaaatcact tgtacgacca ctattcccaa ttgttgataa aagtcctaaa agtccaaata
    27301 tatggtcacc atgtaaatgg gttatacaaa tcaagtctat atctttaaag ccacattttt
    27361 ttagcttcat agatacctga gtaccttcac cacagtcaat tagtattttt ctacctttat
    27421 agtttataaa tacagaggat agaaatctgt ttggcatagg tacatttcca ccacatccaa
    27481 gtaaagttaa atctatcatt ttttcccctt tctaaaatta acacaaatct gttgtttgtt
    27541 tttattatga tatcctatta taattatttt aacattaaat ataacaataa atgtgtattt
    27601 gagtcaagaa atattgtcaa ataaagtttt tatatagaaa tgtataaaaa tttggaaaac
    27661 aaaaagtgaa aattatattt aggagcattt aaaggaggaa aatttaatga aagtattaaa
    27721 gaaaattact attttgtcat tgattacatt aaccttagtt ggttgtagta acggagaaga
    27781 taaaaaagtg gttgaaggtt ctaagaaaat tcaaaattct aaaaaagatg aacagcctat
    27841 aatagtacat aaagatgcaa atatattgaa agataggtat gagccaccaa aaggatttaa
    27901 aagagttgat gtagaaaaag gtagttttga atcatttctt aggaatacta agctaaaaaa
    27961 ttatggagag aaagtaaagt attatgatgg aagaactaag gaatcaaaag gtatttatga
    28021 tagtgtattt gatgttgata ttggcaatag agatttacat caatgtgcag atgcaattat
    28081 gcttatgaga gcagaatact tgtatcagaa taaactatat gatgaaataa gttttgattt
    28141 tgttgatggt tttaaggcga agtattcaaa atgggcacaa ggatatagaa tttcagtaaa
    28201 agatagtggt tcaagctggt ataaagcaac tgaatattca acatcttatg agagttttag
    28261 aaagtttatg gatatagtgt ttgcatattc aggaacgtta tctcttgaaa aagaattgga
    28321 gcctgtaaaa gttgaggata tgaaaatagg agatgtgttt atagttggtg gaagtccagg
    28381 acatgcaagt ataattatgg atatggcaga aaatgaaaag actggtgaaa aggtatttat
    28441 gatagcccaa agctatatgc ctgcacaaca aacgcaactt cttattaatc gtaaaaatcc
    28501 aaatttgagt ccttggtata gtttagattt tgaaggtgat ttgacaatac ctcaatggac
    28561 ttttaaaaga gaccagttga aaagatttta aaataataat tttatataat aaaagaaaaa
    28621 tatagatgtg aaaaaatgta attagat
//
LOCUS       DACXMZ010000024        27863 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.024, whole genome shotgun sequence.
ACCESSION   DACXMZ010000024 DACXMZ010000000
VERSION     DACXMZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27863
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.024"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(121..894)
                     /locus_tag="JD016_04165"
     CDS             complement(121..894)
                     /locus_tag="JD016_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HAU4998822.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKIL"
     gene            complement(894..1301)
                     /locus_tag="JD016_04170"
     CDS             complement(894..1301)
                     /locus_tag="JD016_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAU4998823.1"
                     /translation="MACETIKMEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVA
                     VSEACTNAIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLG
                     LFIIKTLMDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1616)
                     /locus_tag="JD016_04175"
     CDS             complement(1305..1616)
                     /locus_tag="JD016_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HAU4998824.1"
                     /translation="MNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNMLD
                     VKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFKVE
                     G"
     gene            complement(1634..1894)
                     /locus_tag="JD016_04180"
     CDS             complement(1634..1894)
                     /locus_tag="JD016_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAU4998825.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="JD016_04185"
     CDS             complement(1894..2190)
                     /locus_tag="JD016_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAU4998826.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="JD016_04190"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="JD016_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAU4998827.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="JD016_04195"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="JD016_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAU4998828.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="JD016_04200"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="JD016_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAU4998829.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="JD016_04205"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="JD016_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAU4998830.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="JD016_04210"
     CDS             complement(8083..9189)
                     /locus_tag="JD016_04210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAU4998831.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10750)
                     /gene="dnaA"
                     /locus_tag="JD016_04215"
     CDS             complement(9431..10750)
                     /gene="dnaA"
                     /locus_tag="JD016_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAU4998832.1"
                     /translation="MDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILLA
                     PSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYPN
                     LNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHHI
                     VSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERTQ
                     EEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIAI
                     LRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEAL
                     KDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTDL
                     SLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /locus_tag="JD016_04220"
     CDS             11409..11546
                     /locus_tag="JD016_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAU4998833.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="JD016_04225"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="JD016_04225"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAU4998834.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="JD016_04230"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="JD016_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAU4998835.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="JD016_04235"
     CDS             12283..12990
                     /locus_tag="JD016_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HAU4998836.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="JD016_04240"
     CDS             12959..13588
                     /locus_tag="JD016_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HAU4998837.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="JD016_04245"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="JD016_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAU4998838.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15174..17069
                     /gene="mnmG"
                     /locus_tag="JD016_04250"
     CDS             15174..17069
                     /gene="mnmG"
                     /locus_tag="JD016_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAU4998839.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGDDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRR
                     TRGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="JD016_04255"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="JD016_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAU4998840.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNENYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17944..18729
                     /gene="noc"
                     /locus_tag="JD016_04260"
     CDS             17944..18729
                     /gene="noc"
                     /locus_tag="JD016_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HAU4998841.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18864..19637
                     /locus_tag="JD016_04265"
     CDS             18864..19637
                     /locus_tag="JD016_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAU4998842.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19639..20502
                     /locus_tag="JD016_04270"
     CDS             19639..20502
                     /locus_tag="JD016_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAU4998843.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20535..21677
                     /locus_tag="JD016_04275"
     CDS             20535..21677
                     /locus_tag="JD016_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAU4998844.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(22102..23001)
                     /locus_tag="JD016_04280"
     CDS             complement(22102..23001)
                     /locus_tag="JD016_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAU4998845.1"
                     /translation="MSIYGYTIFETVLEQNSFIKAASILNLTPSAVSHAIAKLERDVG
                     FQLFIRNRNGISLTREAEKILPHIRILLHSNEILEQEINRISRSESGIIRIGTFNSIT
                     INWIPEIMKSFRQKHPNIEIHILQGNYINILEWIETSIVDLAFISNTVLPPHIESIPL
                     HKDRIICVFPKGFVPCNPGYVTIEDIQNMSFVMPKKDYDQEVEKILREYHLNVYSQFH
                     IDTDSAIVAMVEAGFGIAILSELIYKSCVGNVDTYPFVPEHYRVISLAYPNSQYDSFA
                     VQKMKEHIVSYTKEKGFFNFKSF"
     gene            23271..24467
                     /locus_tag="JD016_04285"
     CDS             23271..24467
                     /locus_tag="JD016_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAU4998846.1"
                     /translation="MVKFAKRMENMKNSANIVRNLFNSMTDPEIISFGGGAPAIEALP
                     VDIVREISNEVLAKNKRGIEALQYSHPYGLKDLREAVAQKLLEPKGVHASLEDIMIVN
                     GGLETMNLICQVFIDPGDVILVESPTFVHCVEIFEMFQAKCIAVDMDEHGIIPEDIER
                     KIQKHHPKMVYVIPTFHNPTGRTLTTERRKILAGIGSKYDVLILEDDPYRDLRYSGEE
                     LPPIKAFDQTGHTILANSFSKIFSPGARLGYVLAEHAIIERLLDAKTATNSHTSAISQ
                     VLCAEFFNRGYFDEHLKNIRDIYRERRDVMIESIDKYLPKGTKRVFPDGGLFTWVELP
                     ENINTTELLKEAVEHKVAFVAGEGFFTEGGGKGRNCMRISFGNVPPEKIRIGMERLGQ
                     LIASKM"
     gene            24535..25281
                     /locus_tag="JD016_04290"
     CDS             24535..25281
                     /locus_tag="JD016_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HAU4998847.1"
                     /translation="MTNKVPNLWNQLENLKKSYKWVDLSREVSPETPHWTGFPAMEVE
                     PFYLLDRGDGFNVNKYTLVTQYGTHVDAPIHFVADRKYLDEYTPMDMVLPLCVIDKSN
                     EVAKNSDYVVTVEDIQAWEKEYGNIPEGAFVAFRSDWSKRPDDSLDNYDSDGNKHFPG
                     WSIEALQYMVEKRNIAAIGHETCDTDAPILSNVSGYQAETYILGENRYQVEFLVNLSE
                     LPAIGAIIFVTFPRIHKASGFTARCFAICP"
     gene            25314..25817
                     /locus_tag="JD016_04295"
     CDS             25314..25817
                     /locus_tag="JD016_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAU4998848.1"
                     /translation="MSKKAMFVWCVGLGLLDGIYCILCSYLPHIQNYMWIGFISLPIY
                     FCGGAKLEEMPKYFCCAASGIFWGGLTLVVLDIGFISNIHLNMLVFVTIVVCIACFVH
                     LGVLTENVWKGLFSSCPMMFGGFAAIFSQGLAEWFWVLVTLTLGLIFGCIMASISTPI
                     TKWIDKN"
     gene            26065..26898
                     /gene="panB"
                     /locus_tag="JD016_04300"
     CDS             26065..26898
                     /gene="panB"
                     /locus_tag="JD016_04300"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAU4998849.1"
                     /translation="MKNTIQTFKNAKSEGKKLSMLTAYDYSMAKIMDECNINGLLIGD
                     SLGMVVKGEENTLSVTIDEIIYHTKAVKNGAKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LVKEGGAHAVKLEGGANVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGITSEA
                     AKQLIEDAKLIEKAGAFSIVLEGVPAKISEIITNSISIPTIGIGAGINCDGQILIYQD
                     MLGIFEDFVPKFVKQYANVGDIMKDSIKNYILEVETGAFPQEKHSFSIKESELEKLYE
                     D"
BASE COUNT        10069 a         3901 c         4143 g         9750 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaaatt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca acgagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttccta
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactataatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcat gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt tcaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtatt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtat ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcgt taaatccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aaccgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag gatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctgagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctatta ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttactag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaagta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaagaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga attttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac caaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa atctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa aaagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt ctggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atagtggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaat tcctatacag gagaagatgt tatagaaatt aattgccatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 aggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga cattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataagag atactgatga tattgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagacttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaagaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaacttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggc ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcatta gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gccttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggtg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacat cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aaaggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 ttgagaactt atttagtgca gggcaattta atggaacttc tggatacgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccatttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gtttctaaat aaaaaggctg ctgtagagaa agaatttgaa agactaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaaaggc gctggagatg atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ctagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatctgg tgtttctcca gctgatatat cagttttact tatataccta gaacaaatca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgaatgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aaaaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaaaatgaaa actataggga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaagtgat ggaaacacca gttaagtacc ctagaaaagc tggtaaacca
    17761 gccaaaaatc ctattaagta atgaaaaatt tattattggt aagcttaatt atttctcagg
    17821 aaataattaa gcttactgta aaaaaacaaa aataaaggaa ttattaaagt agatgcagaa
    17881 ataaatttgt atgtacatat agagaacaaa aataatttat atctaaaata agagaggtat
    17941 gttatggaag ataaaagagt tatggagata cctatagaag atgtagttcc aaatccatat
    18001 caaccaagaa aaatattttc acaagtttca ttggaggaat tgagcaattc tataaaggta
    18061 tatggtataa ttcaacctat aacagttaga gcaaaagatg gcaaatatga actaattgca
    18121 ggagaaagaa gattaagagc agctaaatta gcagagctta aaaccatacc agcaattata
    18181 aataatatga acgatgaatc ttctgctgtt ctagcgttat tagaaaattt gcaacgagaa
    18241 gacttaaatt tcattgaaga agcaattggt tatgaaaact taataaaaga acatgctttt
    18301 actcaacagc aattagcaga aaaattgggc aaaaatcaat ctactgtagc aaataagctt
    18361 agaatattaa agcttccaaa cgatataaaa atgaagctaa tagagaataa tttaacagaa
    18421 agacatgcta gagcatttct taaactacca agtgaagatt taatgcaaag tgtgttagac
    18481 aaagtcataa aaaatgaatt gactgttaaa aaaacagaaa aattgataca agatgtatta
    18541 gaagaaacta aagttcaaga agagcctgat aaaaaacaga atataaaggg tgctatgagt
    18601 ataagaattt acataaatac tataaagcag gcctttgatg ctatatcaaa tacaggaatt
    18661 gatgctaagt ataatgaaat agataaggga gattatatgg aagttgttgt aaaaatccct
    18721 aaaaaataga gattactgct taccagtaat ctttttttgt aatataagcc ttgtaatata
    18781 aattgattta tagacattat ttatacttaa aaaacaccta ttcaacattt aaaattaatt
    18841 taattcttaa aggggggaac atcatgggca aagttatagc tgtattcaat caaaagggtg
    18901 gagtcgggaa aactacaaca aatgtaaatt taagtgctag cttaggaact ttgggaaaga
    18961 aaatattagt attagattta gacccacaag gaaatacaac tagtggatat ggtattaata
    19021 aaaacgaagt agaaaataca atctatgaaa ttatgttaga tgggttacat attaaagaag
    19081 caataataag tactgaattt gagaatgtag atgtagttcc atcagctact gaactatcag
    19141 gtgcagagat agagcttaca tctaaaacta atagagagta tattttaaaa aattccataa
    19201 aagcggtaat agatgaatat gattatatat ttttagattg tccaccctca ctggggatgc
    19261 ttacaataaa ttgtttaact gctgtagata gtgttttaat tccaatacaa tgtgaatatt
    19321 atgctttaga aggtgttagt caattaatgg agactataaa attagtaaaa tcaagattaa
    19381 atgctgatat agaaattcaa ggtgttgttt taagtatgtt tgatggaaga gctaatttat
    19441 caatacaagt agttgaagaa gttaagaaat actttaaagg tagtgtatat acaactttaa
    19501 ttccaagaaa tgtaagactt gcagaagctc caagtcatgg aaaacctgta atttattatg
    19561 ataaaagatg tagagggtct gtagcttatt tggagttagc agaagaattt atagatttag
    19621 aagaggagga atggtagtat ggagaataag tctaaaagaa gtaatagatt aggtagaggt
    19681 cttagtgcat tgataccaga aattaaagga gaaacttcag aaaaagaaat agtcaacata
    19741 gatatagaca aaatatatcc aaacgaagtt caacctagaa agcaatttga tgaagaaaag
    19801 ataaaagtat tgtcggactc tataaaaaat tatggtgttt tacagccaat agtggttaaa
    19861 atggatgaaa ataataaata tatgataata gctggagaaa gaagatttag agcatcaaaa
    19921 cttgcaaata aaaatcaaat acctgctata attaaagata tagatatgaa agatataatg
    19981 gagatagcct taattgaaaa cttacagagg gaagatttaa attcaataga agaggctcta
    20041 gcatataaga gtttaataga gcattacaat gtaactcagg aagaaatatc agaagctgtt
    20101 gggaagagta gacctcatat aactaataca ctaagacttt taaatcttgg acaagatgtt
    20161 atagaaatga tagacagtgg tagaatcaca gcaggccatg gtaaggcttt attgagaatt
    20221 gcagataaag atttgcaact tcaaatagcg aaaaaaatag aagaagaaga actttctgta
    20281 agagaagtcg aaaatatagc taaaaaaata tctgaaaata aacaagaaga acctaaaaaa
    20341 tctaaaccta aggatgtatt tattttagat gttgaagata aacttagaaa tatatttgga
    20401 acaaaggtga atatttctaa aggaaagaaa aaaggtaaaa tagagataga atactataat
    20461 gatgatgatt taaatagtat tgtatcaatg cttcttgaat agaacaaaaa tataaaacag
    20521 agaggaagat tgttatgatt tatttagata atgctgcaac tacatatcca aaaccagaaa
    20581 gagtgtataa tgctgtttta gattgtatga aaaattattg tgcaaatcca ggtagagcag
    20641 gccataaact tgctatgaga gctgctaggg agatatatga tacaagagag aatatagcta
    20701 agttgttcaa tgtaagtaat cctatgaata tagtatttac atctaatgca acagattctt
    20761 taaatttagc gataaaaggt gtattacaag aaggagacca tgtaataaca acgtctatgg
    20821 agcataattc agtaataaga cctataaaag cattggaaaa aaggggtatt gaaaatactg
    20881 tagtaaaatg cgattatgag ggatttttag attatgaaga tttagaaaaa tcaataaaga
    20941 gtaatactaa actgattgta acaacacatg cgtctaatgt atgtggaact ttaatagata
    21001 taaaaaaagt tggagaaata gctaaaaaac ataatatact atttttagta gatgcatcac
    21061 aaacagcagg tgtttatgat atagacgtaa atgaatgtaa tatagatatg ttggcaatgc
    21121 caggtcataa atgcttattt ggacctcaag gaacaggtat attatatgta agagaagggt
    21181 tgaacttaaa tatattaaaa gaaggtggaa caggtagtaa atcagaagaa atagttcaac
    21241 cagagttatt cccagataaa tatgaatcag gaactcataa tactccaggt atagcaggtc
    21301 ttaaccaagg tatactattt atatttgaaa gaggaatcaa caatataaga caacatgaag
    21361 aagaattatg tcaatatatg atagacaaat tagaggaagt accagatata aagatatatg
    21421 gaccaaagga tagtaaaaaa agagcatcag taatcgctct aaatatagga gatatggatt
    21481 ctggagaggt tactttctta ttagatagtg attataatat agcgactaga tcaggaatac
    21541 attgttcacc actagcacat accactttag gtactttaaa acaaggtgca gtaaggttca
    21601 gtataggata tttcaacaca aaagatgaga tagataaagc agtggaagct ttgaaaaaaa
    21661 tttcaaaaaa taaatagcat gagatttaat aatatagaaa taaaaaatgg ggatgtttca
    21721 aaataaaaat ttgaatagag tattgaaatt tttatgtata ttctttaaat aaaaacttca
    21781 atactctatt aattaaatta ctaattttgc taattttcat aaaatctgtt aaattactca
    21841 taaagttata tattttcaaa ggctcaagtc ttgtatgaaa ttacttgttt taatttttta
    21901 tataactatg acttttgcaa tctttttagt tgaaaagcgc cttcaatcat actaaaataa
    21961 taatattaca atgctaaatt attttatcct ctattataat tataaataca aaccggtgtg
    22021 actcatctta tgaaaaaagt attgttcagc gttgataaaa ctgacaatta agtaataaaa
    22081 atattatact aattttactt tttaaaaaga cttaaaatta aaaaaaccct tttcttttgt
    22141 ataacttaca atatgttctt tcattttttg aactgcaaaa gaatcatatt gtgagttggg
    22201 ataagctaaa ctaattacac ggtaatgctc tggtacaaat gggtatgtat caacattgcc
    22261 aacacaactt ttataaatta gttctgataa aatagcaatt ccaaaccctg cttctaccat
    22321 ggcaacaata gcactatcag tatctatgtg aaattgagag tacacattaa gatgatattc
    22381 acgaagaatt ttttctactt cctggtcata atctttttta ggcataacaa aggacatatt
    22441 ttggatatcc tctattgtaa catacccagg attacaaggt acaaatcctt ttggaaagac
    22501 acaaatgata cggtctttat gaagcggaat agattcaata tgtggaggaa gtactgtgtt
    22561 ggatataaaa gctaaatcca caatagaagt ttcaatccat tctaggatat taatatagtt
    22621 cccttgaaga atatgtattt caatattagg atgtttctgc cgaaatgatt tcataatctc
    22681 agggatccag tttattgtaa tactattaaa agttccaata cgaataatac cactttctga
    22741 acgtgaaata cgattaattt cttgttctaa aatttcgtta ctatgtaata aaatacgaat
    22801 atgaggtaaa attttttcag cttctcttgt taaactaata ccatttctat ttctaataaa
    22861 caattgaaaa cctacatctc tttctaattt tgcaatcgca tgacttacag ctgacggagt
    22921 taagtttaaa atacttgctg cttttataaa actattttgc tctaaaactg tttcaaaaat
    22981 agtatatcca taaattgaca tatgatttac tcctttcctt tagatgagta aaaactcatc
    23041 taaaacgata aaaagattcg ctttatttta taataataat ctactattat taaattataa
    23101 atacaagtga tcatttgcat tttttactca agataataaa aatagtacca cccaaaatac
    23161 aagaattaaa atattatttc tgtttaaaag tgagcggaaa cggaaccttt gctcatctaa
    23221 tctcatataa ttacagacaa atatcaactt gataaaaagg aggaactaat atggtaaaat
    23281 ttgcaaaaag aatggaaaat atgaaaaatt cagcaaatat tgttcgtaat ctttttaatt
    23341 ccatgacaga tcctgaaatt atatcttttg gcggaggtgc accagcaata gaggctttgc
    23401 cagttgacat tgtaagagaa atttctaatg aagttttagc aaaaaacaaa agagggattg
    23461 aagccttaca gtacagtcac ccatatggtt taaaagattt acgtgaagca gttgctcaga
    23521 aattattaga accaaaagga gtacatgcaa gtctagagga cattatgatt gtaaatggtg
    23581 gtttggaaac aatgaatctg atttgtcagg tattcattga tccaggagat gtcattttag
    23641 tagaatcacc aacatttgta cattgtgttg aaatttttga aatgtttcag gcaaaatgca
    23701 tagcagtaga tatggatgaa catggaatca ttccagaaga tatagaaaga aagattcaaa
    23761 aacatcaccc aaaaatggtt tatgtaattc ctacttttca taatccaact ggcaggacat
    23821 tgactacaga acgaagaaaa atattggcag gtattggtag taagtatgat gttttgattt
    23881 tagaagatga cccttatcgt gatttgagat atagtggaga ggagttacct cctattaaag
    23941 catttgatca aacaggacat actattttag caaatagttt ttctaaaatc ttttctccag
    24001 gagcaagact tggttatgtt ttagctgaac atgcaatcat tgaacgatta cttgatgcaa
    24061 aaacagcaac taattctcat acatcagcta tttctcaagt attgtgtgca gagtttttca
    24121 atcgtggtta ctttgatgaa cacttgaaga atattcgaga tatttataga gaacgcagag
    24181 atgtgatgat tgaaagcatt gataagtatt taccaaaagg tactaaaagg gtatttccag
    24241 atggtggttt atttacttgg gtagagctac ctgaaaatat caatacaact gaattattga
    24301 aagaagcggt agaacataaa gtagcttttg tcgcaggaga aggattcttt acagaaggtg
    24361 gtggaaaagg gcgaaattgt atgagaatta gctttggcaa tgtacctcct gaaaaaatta
    24421 gaattggtat ggaaaggctt ggacaactaa tcgcttccaa aatgtaaata attacttata
    24481 taaaatacaa ctttatattt taagatttat aatataacca aaggaggaat acttatgaca
    24541 aataaagtac caaacttatg gaatcaattg gaaaacttaa aaaaatcata taaatgggtg
    24601 gatttgagtc gtgaagtaag tcctgagaca cctcattgga caggctttcc tgcaatggaa
    24661 gtagaacctt tctacttatt ggatagagga gatggattta atgtaaataa atatacacta
    24721 gtaactcaat atggaacaca tgtagatgca ccaattcatt ttgttgccga tagaaagtat
    24781 ttggatgaat atacgccaat ggatatggta cttccacttt gtgttattga taagagtaat
    24841 gaggttgcta aaaattcaga ttatgtagta actgttgaag atattcaagc atgggaaaaa
    24901 gaatatggta atattccaga gggtgcattt gtggcatttc gttcagattg gtcaaaaaga
    24961 cctgatgatt ctttagataa ttatgattca gatggtaata agcattttcc aggatggagt
    25021 atagaagctc tgcaatatat ggttgagaag agaaatattg ctgcaattgg acatgaaact
    25081 tgtgatacag atgcaccaat tttatcaaat gtaagtggat atcaggcaga aacatatatt
    25141 ttaggagaaa atcgatatca agttgaattt ttagtaaatt tgtctgagtt acctgcgata
    25201 ggagctatta tttttgtaac attcccaaga atacacaaag catctggttt tactgcccgt
    25261 tgttttgcaa tttgcccata aggttttaac tgttagaagg gagggggaga cagatgagta
    25321 aaaaagcgat gtttgtttgg tgtgttggct taggtctact agatggcatc tattgtattc
    25381 tttgttccta cttaccccat atacaaaact atatgtggat tggttttatt tctcttccaa
    25441 tttacttttg tggaggtgca aaattagaag agatgccaaa atatttttgt tgtgcagcaa
    25501 gtggtatatt ttggggtggg ctaacattag ttgtattaga cataggattt atatccaata
    25561 tacatttaaa tatgcttgtg ttcgtaacaa ttgttgtatg cattgcttgc tttgtacatc
    25621 taggtgtatt gactgaaaac gtatggaaag gattgttctc aagttgccca atgatgtttg
    25681 gtggatttgc agcaattttt tctcaaggat tagcggagtg gttttgggta cttgtaacac
    25741 ttacactagg gttgattttt ggttgtatca tggcttcaat ttcaacacct atcacaaaat
    25801 ggattgataa gaattgagat aaagtagcta ttcaataatt tttaaaaagt taatttattt
    25861 atgaaatata atggtatatt atttcaagat ggtttaaaag gaaaagtttt ttaatacttt
    25921 tccttttaat ttaaaatata aatacatttg ccacataagt gcttctttaa aatctagtat
    25981 tatctttact agatatagga gtaagttgtt ttaccaaatt agttttatat gaagatattt
    26041 acaaaatttt aggaggaaaa aacaatgaaa aatactatac aaacttttaa gaatgctaaa
    26101 agtgaaggaa aaaaactatc tatgcttact gcttacgatt actcaatggc aaaaataatg
    26161 gatgagtgta atataaatgg tcttttaata ggagattcat taggaatggt tgtaaaaggc
    26221 gaagaaaata ctctctctgt tactatagat gaaattatat atcatacaaa agctgtaaaa
    26281 aatggggcaa aaaatgcttt aatagtaagt gatatgcctt ttctatctta ccatgtttct
    26341 atagaagatg ctgttaaaaa tgctggtaga cttgttaaag aaggaggagc tcatgctgta
    26401 aaattggagg gtggagcaaa tgttataaag caaatagaaa gcattgtaaa tgctcaaata
    26461 cctgtaatgg gtcatttagg actaactcct caatctgtaa attcatttgg tggatttaag
    26521 gttcaaggaa ttactagtga agcagcaaaa caacttattg aagatgcgaa attaatagaa
    26581 aaagcggggg cattttctat tgttcttgaa ggtgttcctg cgaaaatatc tgagataata
    26641 actaactcta tttcaattcc tacaataggg ataggagcag gaattaattg tgatggacaa
    26701 attttaattt accaagatat gttaggtata tttgaagatt tcgtacctaa gtttgtgaag
    26761 caatatgcaa atgtaggaga tataatgaaa gattcaatta aaaattacat actagaagtt
    26821 gaaacagggg catttccaca agaaaaacat agtttttcaa taaaagaatc tgaattagaa
    26881 aaattatacg aagattaagg atgttataag ctgtcatata taacaattta ttattatatt
    26941 tagagatgga ttgtccatct tttctggata aaaatttatc cagttttatg attataataa
    27001 ccaattgtaa aatatatcta aacaatatat tatactatgt agatttttca atgaaataac
    27061 atcagatatt tactgaatat ttttatttct tattttttag aggaacttct tttaatttaa
    27121 atataaaaat aatgaatgtg tttatatagt aactgtatat attaatagtt taaaaggaca
    27181 ctattcattc gtaaaaactt aaattgatga ttttattaaa aaatatgtag aagatattgt
    27241 aggtttatgt cttcatagaa taatgattat attatatgaa tgtgatttag cataaaacta
    27301 gatatattaa agttgagtga gaagaatctt gaacaaagtt cttcttacca actttaatat
    27361 atttctatta tacaatctat tttttatcat tattgaaatt gtttacaact tgatttaact
    27421 caattaatat acttccaact tccatataga gaattttact ataaacacaa gtaatacaat
    27481 aggaatgtaa ttatcacttg atttttttat tttttaaatc ccataacaaa acattaagtg
    27541 aaactattgg agttaacaat tgttgtagta taaaagctat taagttatct atatattatc
    27601 ttcctaaaaa agttttctta acactttaat tattattcaa aaactattta aacaaagcta
    27661 taagagagtt taagctatca ctccaataag atttattctt tattttatac atctaatatt
    27721 tcaaattcgt aaatttactt ccaattatag aattatactt gtttcgtatt tattcaattt
    27781 atcttataaa agcttttgtt ttgtaaaaat attaatttta tattaaaaca agacttagaa
    27841 actatatact tcttgaaaaa att
//
LOCUS       DACXMZ010000025        27611 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.025, whole genome shotgun sequence.
ACCESSION   DACXMZ010000025 DACXMZ010000000
VERSION     DACXMZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.025"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            279..623
                     /locus_tag="JD016_04305"
     CDS             279..623
                     /locus_tag="JD016_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAU4998850.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            654..2255
                     /locus_tag="JD016_04310"
     CDS             654..2255
                     /locus_tag="JD016_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HAU4998851.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENMKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2265..3374
                     /gene="dprA"
                     /locus_tag="JD016_04315"
     CDS             2265..3374
                     /gene="dprA"
                     /locus_tag="JD016_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAU4998852.1"
                     /translation="MDFMEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKE
                     IYNLKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPK
                     LLFYKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAY
                     SHIGCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFS
                     NRNRIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIK
                     EGAKLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSIC
                     DYTGIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3407..5494
                     /gene="topA"
                     /locus_tag="JD016_04320"
     CDS             3407..5494
                     /gene="topA"
                     /locus_tag="JD016_04320"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAU4998853.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5659..6444
                     /gene="codY"
                     /locus_tag="JD016_04325"
     CDS             5659..6444
                     /gene="codY"
                     /locus_tag="JD016_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAU4998854.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6551..7816
                     /locus_tag="JD016_04330"
     CDS             6551..7816
                     /locus_tag="JD016_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAU4998855.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDVEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7847..8617
                     /locus_tag="JD016_04335"
     CDS             7847..8617
                     /locus_tag="JD016_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU4998856.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8726..9172
                     /locus_tag="JD016_04340"
     CDS             8726..9172
                     /locus_tag="JD016_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAU4998857.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9190..10383
                     /gene="nifS"
                     /locus_tag="JD016_04345"
     CDS             9190..10383
                     /gene="nifS"
                     /locus_tag="JD016_04345"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HAU4998858.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEESIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10437..10877
                     /gene="nifU"
                     /locus_tag="JD016_04350"
     CDS             10437..10877
                     /gene="nifU"
                     /locus_tag="JD016_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HAU4998859.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11160..12245
                     /gene="mnmA"
                     /locus_tag="JD016_04355"
     CDS             11160..12245
                     /gene="mnmA"
                     /locus_tag="JD016_04355"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAU4998860.1"
                     /translation="MFMNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDV
                     VEIEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCI
                     ACNKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYN
                     LTQEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSK
                     KRIEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDN
                     EDLFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQ
                     RAITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12388..12626
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12694..15333
                     /gene="alaS"
                     /locus_tag="JD016_04360"
     CDS             12694..15333
                     /gene="alaS"
                     /locus_tag="JD016_04360"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAU4998861.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15358..15615
                     /locus_tag="JD016_04365"
     CDS             15358..15615
                     /locus_tag="JD016_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAU4998862.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15625..17796
                     /locus_tag="JD016_04370"
     CDS             15625..17796
                     /locus_tag="JD016_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998863.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18002..18427
                     /gene="ruvX"
                     /locus_tag="JD016_04375"
     CDS             18002..18427
                     /gene="ruvX"
                     /locus_tag="JD016_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAU4998864.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18505..18792
                     /locus_tag="JD016_04380"
     CDS             18505..18792
                     /locus_tag="JD016_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAU4998865.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19074..19532
                     /locus_tag="JD016_04385"
     CDS             19074..19532
                     /locus_tag="JD016_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAU4998866.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19649..20386)
                     /locus_tag="JD016_04390"
     CDS             complement(19649..20386)
                     /locus_tag="JD016_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAU4998867.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20603..22279
                     /locus_tag="JD016_04395"
     CDS             20603..22279
                     /locus_tag="JD016_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAU4998868.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22485..22685
                     /locus_tag="JD016_04400"
     CDS             22485..22685
                     /locus_tag="JD016_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HAU4998869.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22739..23902
                     /locus_tag="JD016_04405"
     CDS             22739..23902
                     /locus_tag="JD016_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAU4998870.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(24113..24535)
                     /locus_tag="JD016_04410"
     CDS             complement(24113..24535)
                     /locus_tag="JD016_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HAU4998871.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(24556..25392)
                     /locus_tag="JD016_04415"
     CDS             complement(24556..25392)
                     /locus_tag="JD016_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAU4998872.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            25690..26286
                     /locus_tag="JD016_04420"
     CDS             25690..26286
                     /locus_tag="JD016_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HAU4998873.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLLYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
     gene            26316..27035
                     /locus_tag="JD016_04425"
     CDS             26316..27035
                     /locus_tag="JD016_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HAU4998874.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            27037..27576
                     /gene="scpB"
                     /locus_tag="JD016_04430"
     CDS             27037..27576
                     /gene="scpB"
                     /locus_tag="JD016_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAU4998875.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVVTSE"
BASE COUNT        11187 a         2903 c         4986 g         8535 t
ORIGIN      
        1 ggatacaatg tattttcaac atttttcaaa tagcttacaa ctaatataaa taaagataaa
       61 tatcgtataa aagcaactaa tttttctctg ttcattaaag gcactcctcc aaaaaaataa
      121 acttttccta tgaaaactag aataatatca taaaagaata aagtcaataa aagtatttat
      181 taatattgat ggaaataaaa tcaatccaaa aacttgaata gtaaaattgt atactgacca
      241 taatttaaaa gtgaaaattt aatgtattgg agtgaataat gaataataaa gaaaaaggtg
      301 attttggaga aaaagtagca gttaattatt tgctatcaaa aggagcaaag atactagaaa
      361 aaaattacag attaaagata ggggaaatag atataatagc aaaaatggaa gatgaaatta
      421 tatttgtgga ggtgaaatca aggtctaaca taaagtttgg ctacccttgt gaatctgtaa
      481 gcttcaaaaa aagaaaaaag ataattggag ttgcaaatta ttatattata aaaaataact
      541 taaataacac tcccataaga tttgatgtta tagaggtata tttattagaa aaaagaataa
      601 atcacataat gaatgctttt taaaaaagat aatgtttttg gaaaggatta aatatgttaa
      661 gtattattaa ttcaagcaac ttagtaggaa tcgacagctt tttagttaag gttgaagtag
      721 atgtaagcaa tggaatacct agttttaata ttgttggtct tccaggcaaa gaaattaaag
      781 aagcaagaga aagagtaaag tcagcaattc ttaatagtgg atataaattt ccaagcacga
      841 gaatagttgt aaatttatca ccagcagata taaaaaaaga aggagctttt ttagatttat
      901 ctataagtat aggcctttta agagagttaa taaaaaaaga tgaaaattac ataagagaaa
      961 gtatgtttat aggagaatta tcattagatg ggaaaataag aaaagtaaga ggtatattgc
     1021 ccataattat gggtgcaaaa actcagaaca taaaacgtat ttttatacca attgaaaata
     1081 tgaaagaaag tctatttgta gatgaaattg atataattcc tattaaaagt ttaaaagaat
     1141 gtgtcgattt tttaaacgaa gagataaaag tcgataaagt aaggataatg tcttttttgg
     1201 atgataagtc aagaaaagag aacgaagaat tagaaaaaga taatagttat atagattgca
     1261 aatatactaa gattaataat gaagaaagta aatatgatga agactttaaa gatgtaaaag
     1321 gaaattattt tgtaaagagg agtgcagaaa ttgctgctgc tggaaatcat aatatgttta
     1381 tgattggacc tccaggctca ggaaaaacta tgattgcaaa aagagtaagg acaattttac
     1441 cagatataag tgtagaagaa atgatagaag taagtaaggt ctatagtata ttgggtatga
     1501 taaatgaaac taaaggaata attgataaaa gaccttttag agcacctcat catacaacta
     1561 caaagcaatc tttaattgga ggaggaatgg atgcaagacc tggagaaatt gctttagcac
     1621 atagaggtat cttattttta gatgagatag ccgaatttga tagaaaaata cttgagactt
     1681 taagacaacc tatagaagat ggatatgtaa atatatccag agtaaagtac agtgcaaaat
     1741 atccttgtag agtactgtta gttgcagcta tgaatccatg tccatgtggt tattatatgt
     1801 cagagacaga gtgtagatgt agaagtaacg aaattgatag atacataaat aaaatatcag
     1861 gtccattgtt agataggttt gatatttttg tagaagttaa ttctataaaa tattcagatt
     1921 ttaattcttt aaaacaagaa gaaagctctc aaaaaataaa gagaagagta gaaaatgcta
     1981 gaaaaataca gataaataga tttaagaagg ataatataaa aaataatagt gaaattaaag
     2041 cttacaattt atttaaatat tgtaaactgg aaaaagaagc ttcaaaaacg gctgagatga
     2101 tatttaataa atacaaccta agcagtagaa gttatacaaa acttttaaaa atggcaagaa
     2161 caatagcaga tttagaagaa agagacttga taaattcaca gtgtattata gaagcttttt
     2221 catttagaaa ggcttattat agttatttta aatagttttt tgaggtggat tttatggaaa
     2281 aaagagatac ttatttatgg ttaaaatcaa ttggtggaat aacaacaaaa acaattgaaa
     2341 taatagaaaa tgaaatagta aatattgaag atatatttga tttttctgaa aaagaaatat
     2401 ataatttaaa aaatataagc ttgaatatta gaaaaaatat agtaaaatat agaggtcacg
     2461 cttatttaga aaatataaaa gaactactct acaaaaaagc gattaaatat atttgtaaat
     2521 atgataaaga atatcctgaa aatttaaaga atatatacaa cgctccaaag ttattattct
     2581 acaaaggtga cataggctta gttaataata attttaatat agctatagta ggttcaagaa
     2641 aacctacagc ctatggaatt aattgtgcaa aaacaataag ttgtcaacta tcccaatatg
     2701 gagttaatat agtaagtgga ttagctatag gaatagatgc atactcgcac ataggttgta
     2761 tgagtggaaa atctaaaact atagcagtac ttggttcagg agtagacaat cctcttccaa
     2821 aacaaaattt acatttatca aataaaatat tagaaaatgg gggattatta ttatcagagt
     2881 ataatattaa ctcgacagta gccccgtacc atttttctaa tagaaataga attatcagtg
     2941 gtttaagtga tggagtagtt gttgtagaag cagcaataaa aagtggtgca ctaatcacag
     3001 tagattttgc tcttgaacat ggaaaaaatg tttttgcaat acctggtaat ataaattctc
     3061 aaatgagtag aggttgtcat aaaattataa aagaaggtgc aaaattaatt gaaaatatag
     3121 atgatatttt aaatgaatat aacatattta atattattga taaaaaaata aatcaaaaat
     3181 atgataatat aagtctaaat gccaagtcta agcaaataat tgaagctata aaaagagaag
     3241 gaaacttgca tatagatagt atttgtgatt atactggtat agaaataaag tatgtaaatt
     3301 caataatcaa tgaattagta ctaaatgagt tagtagtaga aatgaataat aaaacgtaca
     3361 gtttaaatgt ataaattaag attaggtagt gggggtgtat ggttttatgg ccaaaacatt
     3421 agtcatcgta gaatcgcctg caaaagctaa aactatagaa aaatttttag ggaaaagtca
     3481 ttacacagtt aaagcatcag ttggtcatgt cagagattta cctaaaagta aattaggtgt
     3541 agatatagaa aataattttg aacctcaata tataaatata agaggaaaag gcgatgtaat
     3601 aaaagaatta aaaaaagaag ctaaaaaatc taaacaagta tatctagcaa ctgaccctga
     3661 tagagaaggt gaagctatat catggcattt agctcatata ctaaatttag atgaatctga
     3721 ggattgtaga atagagttta atgagataac taaggatgca ataaaaaaag caattaaaca
     3781 tcctagaaat attgatataa atttggttga tgcacaacag gcaagaagag tgctagacag
     3841 acttttaggt tatcaaataa gtcctatatt atggcaaaag gttagaaaag gtcttagtgc
     3901 aggtagagtg caatctgtta caacaaagtt aatttgtgac agagaaaaag aaataaaggc
     3961 atttattcca aaagaatact ggacaataga tgttgaggct aagacagtaa aagacaaaga
     4021 tataactttg aaattttatg gtaaaaatga tgaaaagatt gaattagaaa atgaagaagc
     4081 tgtaaatgaa atactaaaag aaatggaagg taaagattta aaggttgaga agatagaatc
     4141 taaatcaaga cgtaaatcag ctccaaaacc atttacaaca agtatgcttc aacaagaagg
     4201 agcaaataag ttatttttta ctactaaaaa aacaatgatg atagcacaag aattatatga
     4261 aggtatcgac atagaaggtg aaggtactgt tggtcttata tcatatataa gaactgactc
     4321 taaaagaata tctgatgaag caaaagaaaa agctaagggc tatatcgtaa acaatttagg
     4381 agaaaactac tataaaaaca ctggtgataa aaaagaaaaa aaggatacta aaaaggttca
     4441 agatgcccat gaggcaataa gaccaacctc agttgataga actcctgaga gtataaaagc
     4501 ctcattgagc aaagaccagt acaagttata caatttaatt tggagaagat ttgtagctag
     4561 tcaaatggaa gattcagtgt ttgatatact aaatgtagaa tgtaaaatag gcgatattgt
     4621 atttaaggca actggttcaa aaatgaaatt tgatggttat accaaggtat ataatttttc
     4681 agaaagagaa gacaaaattt taccaagtat agaaaaagga gatatattaa atatagaaaa
     4741 tatattgcca gaccaacatt ttacacaacc accagcaaga tatacagaag ctagtttagt
     4801 aaaaacacta gaagaactag gtataggtag accaagtacg tatgctccaa caatagctac
     4861 tatactgaat agagagtatg ttgaaaagca aggaacaagt ctatgtccaa cagaacttgg
     4921 aataatagtt actgatattt tggaaaataa tttccataaa tttatagatg tagattttac
     4981 tgctgatatg gaaagtaaac ttgatgttat agaagaaggc aatacagatt ggaaaaagat
     5041 tgtatcagaa tcatatgcac cacttaaaga ggctattgaa gaagctcttg aaaatataga
     5101 aaaagtaaat atggatgaag aaactgatga gatttgcgag aactgtgggt ctaatatggt
     5161 tatcaaatat ggtagatttg gaaagtttat ggcttgtaaa aactatcccg attgtaaaac
     5221 aactagacca attatgaata aaattggtgt taaatgtcca aaatgtgaag aaggagatat
     5281 tatacttaga aaatctaaaa gaggtaaagc tttttatggg tgttctaatt atccaaactg
     5341 tgactttgta gcttggaata agccaacagg agagttttgt gagaagtgtg gttcatatat
     5401 ggttgaaaaa gtgactaaat ctgaaacaaa aacagtgtgt tctaataaag aatgtaataa
     5461 agagacaaaa gagcataatg acggaaaaga gtgaaaattg aaaaaaatca cataattctc
     5521 ttgaaaaagg ttccaatcta tgataatctt tcaagtgtaa acaactaata gaaaaataaa
     5581 ttttcattaa atgtgaaaat aatattagaa aatataaaag gttattaaaa ggcctaaatt
     5641 tcatgaggag atgattaaat ggcaagtgaa gtgttacaaa aaacaaggaa aataaataaa
     5701 acattacaaa caagtggtgg aagcagtgtc tcttttgatt tactggccgg agcattgggc
     5761 gacgttttaa gttctaatgt ttatgtagta agtgcaaaag gtaaagtact aggtcttcat
     5821 ttaaatgatg ttcaagacag ttcagttata gaagatgagt atactaagca aaagaaattt
     5881 tcagatgaat atactcaaaa tgtgttaaaa attgatgaaa cattagaaaa tttaaatggt
     5941 gagaagatat tagaaatctt tcctgaagaa catggaagat tacaaaaata tactacagta
     6001 gttccaatat taggaagcgg tcaaagatta ggaacattgg tactttcaag atattcaaat
     6061 tcattcaatg atgatgattt agtaatagct gaatacagtg caactgttgt tggtcttgaa
     6121 atattaagag caataggtga agaattggaa gaagaaatga gaaagaaagc tgtagttcaa
     6181 atggcaatag gcactctgtc ctactccgag cttgaagcag ttgaacatat ttttgctgaa
     6241 ttggatggaa aagaaggtct acttgtagca agtaagatag ctgatagagt tggtataact
     6301 aggtctgtaa tagtaaatgc acttagaaaa tttgagagtg caggtgtgat agaatcaaga
     6361 tcattaggta tgaaaggtac tcatataaga atacttaatg acaaacttac agatgaatta
     6421 aaaaaattaa aaaacaatca ataatctata ttttataggt ttagattaga aaaggtatct
     6481 atttcaaata gatacctttt ttagacgaat tattaaggta atgataagta tattaaaaag
     6541 gagtgagaaa atgcctttag cagataaaat aagaccaaaa acaatgaatg atgtaatagg
     6601 tcaaagtcat ataataggta atggaaaaat attgtctaag atattacaat ctaatttttt
     6661 accaaatatg atattttttg gtcctcctgg tgttggtaaa actacagtag cggaaataat
     6721 agcagaaaga agcaataaaa gcttctataa aataaatgct actaattcat ctttagaaga
     6781 tataaaaaag gtgatatcag agcttggaag tataaataat gtaaatggag tcctattata
     6841 tatagatgaa atacaaagtt ttaataaaaa acagcaacaa tcaatattag aatttatgga
     6901 aaatgggagt ataactttga ttgctagcac tacagaaaat ccatatcatt atgtatataa
     6961 agctctttta agtaggtcta ctgtatttga atttaaacct ttagaaaaac ttgatgtaga
     7021 aaaaggactg aaaagagctg ttgaagtatt aaatgaagat agctatatgg atattgagtg
     7081 caataatgat gctattgaag atatagcaat tctttctgat ggagatatga gaagagcctt
     7141 aaatattttg gaagtggtag tctattcaac taaaccaaat aaggataata taacttatat
     7201 agattcagat gtaataaggg catctacatt taataaaatt ataaactatg acaaaaatgg
     7261 tgatagtcat tatgatattt taagtgcatt tcaaaaatct ataagaggaa gtgacccaca
     7321 ggcatctata cactatctag caagacttat aaaaggtggg gatttgataa gtatatgtag
     7381 gagactttta gtaatagcta gtgaagatat aggtttagct tatccaaatg ctattgtaat
     7441 tgtaaaggca tgtgtagact cagctatgca acttggattt ccagaagcga aaataccatt
     7501 agcagaagct actattcttt tggctacatc tccaaagtct aattctgcat gtatagcaat
     7561 aatgaaagct atggatgaac tggataaaga gtttgttaag gatataccaa atagtataaa
     7621 ggatgctcac tattctggtt ctaaagaaat aggtaggggt tgtggataca aatatcctca
     7681 taattttaaa aatcattatg taaagcaaca gtatttacca gatagcataa aagatagtat
     7741 ttattacata ccacaagaaa ataaaactga gaaaaatata aaaaaatatt tagaatatct
     7801 aaatagtgat ttttagactg aactttatgt atcgggggta gatattatga ttataaaact
     7861 taactcaact tatcacagta gagttatgaa atacttaaaa aaagaacctg agtacaattt
     7921 gtttataata ggggatattg aaagatatgg atatggaaat aattttttaa atatatgggc
     7981 agatgtgggt gaacatggtg aaataaaggc aatattactt aaatattttg aatttatgat
     8041 gttttattct gatggagagt atgatgttga aggtttttat aatctacttc gtaatactaa
     8101 ttacgaagaa atatctggga aaatatgtgc tgtagatgct ttagcaaaga gattaggact
     8161 taataattta aaggtagttg atttttgcaa acttcaaact aaaaagtttt tgatagataa
     8221 taattgtaat gcaaaggtta aaagaattag gcttggaaat ctaaaaaaga cagtgaaact
     8281 ttatgactta atagatgaat ttcacagtac aactttagag aatttaaaaa atggactaag
     8341 gacaggtaga ggatattgta tagagattaa caaacaggtg gtgtcaatgg caaagagtac
     8401 ttcagaaaat aggactcatg caatgataat tggagtagga acacatccta aatatagagc
     8461 taaaggactt gctacaaaat gcctaattaa actatgttca gagttactga gagaaaacaa
     8521 aattccttgt ttgttttatg ataatgaaga ggctggaaag atatataaaa aactaggatt
     8581 tgaaaacatt ggaaaatggg gaatttattc aaaataaaaa attaaaatta atcttgacaa
     8641 aattagtagg gtataataaa attatcttga aagtaattcc tactaaaaca gtatgaatta
     8701 gatttctata tagaggtgat aatatatgaa gttgtctact aaaggtagat atggactaaa
     8761 agcaatgttt gaattagcat taaatcaaga caatggacca gtatcattaa aatttattgc
     8821 taagaagcaa aaaatatcag atcagtactt agaacaaata ttttcatctt taaaaaaatc
     8881 gggtctagta aaaagtgtga gaggtgctca aggaggttat ttgctttcaa agaatgctga
     8941 agacataaca gtaggagata tacttgtagt attagaagga ccagttgcat tatctgactg
     9001 tgtactagat gaagatgtat gtgaaaactc aaacatgtgt gtaacaaaaa tagtttggga
     9061 aaagatgaaa aagggtatag aagatgttat agattcaatt actcttaaag atatgataaa
     9121 tgactataac aaaaataaat tagaaaatga tataacaaat attaaaaaat agatttggag
     9181 atgaatgtaa tggaaaaaag aagattgtat atggattatt ctgcaacaac acctattaaa
     9241 aaagaagtat tggatgcaat gatgccatac ctaacagatt attttggtaa tgcatctagt
     9301 tttcacacat tcggaagaga agcaaaagat gctttggata aagcaagaga acaagtagca
     9361 gcccttataa atgctgaacc tagtgaaata tattttacag caggtggttc agaaagtgat
     9421 aactggacac tagagggtgt agcttatgct aataaaaaca aaggtaatca tataataact
     9481 tcaaaaatag agcaccatgc aatacttcat acatgtgaat acttagcaaa acatcatgga
     9541 tttgaaataa cttatttaga tgttgatagc gaaggaaaag tagacttaaa acaactagaa
     9601 gagtcaataa aagatactac tatacttata agtataatgt ttgcaaataa tgaaatagga
     9661 actatacagc ctattaaaga aatatccgaa atagctaaaa aacataagat attattccac
     9721 actgatgcag tacaagctac aggtaatatt ccagtagatg ttaaagaatt aggaatagat
     9781 ttgatgagta tgtcttcaca taaaatatat ggaccaaaag gtgtaggagc tttatacata
     9841 agaaaaggtg taagacttca taactttgtc catggtggag cacaagaaaa aagcaaaaga
     9901 gctggtacag aaaatatacc tgcaatagtt ggatatggaa aagcagcaga attagctaag
     9961 gaaaatatgc aaaatcatgt agaaacttta actcgtctta gaaataagtt aatagatggt
    10021 gtgttagaaa gaatacctta cacaagagta aatggtagct tagaaaatag attgccagga
    10081 aatgcaaact ttgcattcca atttatagaa ggtgaaggaa tacttttatt attagatatg
    10141 ttaggaattg ctggttcaag tggttctgca tgtacttctg gttcattaga cccttcacat
    10201 gtacttcttg caataggttt accacatgaa atagcacatg gttcattaag acttacagtt
    10261 ggagatttta caacagatga tgatatagat tatatactag agaacttacc aaaagtaata
    10321 gaaagactta gaagcatgtc accactttat gatgatgcga agaaacaagg tttagtaaaa
    10381 tagacataaa gataaagcat aaaaaataga cataaattga gtaggaggaa aagataatgc
    10441 aatatagtga taaagttatg gaacatttta tgaatccaag aaatatggga gaaattgata
    10501 atgcaagtgg tgtaggtgaa gttggaaacc ctacatgtgg agatataatg aagatatttt
    10561 tagacataga tggagatgta ataaaggatg ttaagtttaa aacatttggc tgtggttcag
    10621 ctatagcaag ttcttctatg gcaactgaaa tgataaaagg aaaaactata aaagatgcat
    10681 tagaacttac aaataaagct gttgcagaag ctttggatgg tttaccacca gtaaagatgc
    10741 attgttcagt tttagctgaa caagctgtaa aagcagctct tatagattat gctcaaaaga
    10801 acaatatcca tataccagaa ttggatggat atgttataga tgatgctcat gaccatgatg
    10861 ttgaagaaga agaataaaaa taagtggttg tgttatgaga agtttacaat gattaaaaag
    10921 aagtactaat gatatagtta ggacataaaa aatagctgtt tttaaaatgg ctatttttta
    10981 ttgcctaaat taagtatttt aacttgattt agaggttaat tagttaacaa aataatccat
    11041 aagattcaaa taataaagat gattattttt ttagaaaata gaacaagcta tattttaaat
    11101 aagtacaata cttgctctat atggtataat aataagtaat gcaaaagatg aggtgaaatt
    11161 tgtttatgaa taaaaaagta atgataggta tgagtggagg agttgatagc tctgtagctg
    11221 catatctttt aaaacaacaa ggatatgatg tcattggtgt tactatgaag ttgtggcaag
    11281 atgatgatgt tgttgaaatt gaaggtggtt gttgctcttt atctgctgtt gaagatgcta
    11341 gaagggtagc aaataaaata ggtataccat tttatgtttt aaattttaga gaagtgttta
    11401 aagaaaaagt aatagattat tttatagatg aatatttgga aggaaaaact cctaatccct
    11461 gtatagcttg taataaacat ataaaatttg atgattttta taagaaggct agacaaatag
    11521 gttgtgatta tgtagctaca ggtcactatg caaagataga aaaggatgaa agtacaggaa
    11581 gatatttgct aaaaaaatct gttacagata aaaaagacca aacttatgca ctttataatc
    11641 ttacacaaga gcaactagag catactttat tgccaatagg tgactatgaa aaagatagag
    11701 taagagaaat tgctaaggaa atgggtatgg cagtacacaa taagcctgat agtcaagaaa
    11761 tttgttttgt taaagacaat gactatgcaa attatgtaaa aaaacattct aaaaagcgta
    11821 ttgaagaagg tttttttgta gatactaaag gcaatatact tggaaaacat aagggtatat
    11881 tatattatac tataggccaa agaaaagggc ttggaattac ttttggaaaa ccaatgtttg
    11941 taatagacat aaatccaata aataatacca tagttcttgg agacaatgaa gatttattta
    12001 aaaaggaact tattgctaaa gatgttaact ttatatcaat agatacgtta gaagaaccct
    12061 taagagttca agccaaaata agatattcag caaaaccatc accagctact attcatagag
    12121 taggagaaga tactattaaa atagtttttg atgaagctca aagagctatt actaaaggtc
    12181 aatctgtagt aatgtatgat ggagatattg tagtaggtgg aggtataatc gagaaaagtt
    12241 tataaaaaca tctaaaaaat taaaaaaata aacaataaag tatattaaga gtaaataaag
    12301 tttataaaat aaatttattg tatatgaaaa tatagtattg attaattttg taaaatttag
    12361 taatataatt agtatattaa ttaaaaaaca aatacagtga taaggaatag taaaatattt
    12421 gatgtcttac agagagaggg aaatgctgga agcccttaga atatgatatt tgaagcagcc
    12481 ttttagtagc aatcttgaac aatttatctt ttataaagaa gatttaagta gagattgacg
    12541 gtagttacgt tatagctaag gttttccgaa gatatcatac tttgtatgat aattagggtg
    12601 gtaccgcgat tgtcctcgcc cctataaatt tatttatagg gacgaggttt tttatttaaa
    12661 cgaaaaaata aaatttatta ggaggaacat gatatggaaa agatgggatt aaatgaaata
    12721 agaagtaagt ttttagaatt ttttgagtca aaaggtcatt atgtagcaaa tagttattct
    12781 ttagtaccaa acaatgataa gagtttgtta cttataaact caggtatggc acctttgaaa
    12841 aactactttt caggtgtaga agtacctcca agtgtgagaa tgtgtacatc tcaaaaatgt
    12901 ataagaacag gagatataga aaatgtaggt ataactgcaa gacatgctac attctttgaa
    12961 atgatgggta acttctcatt tggagattac tttaaaagag agtcaatcaa atggggatgg
    13021 gaatttgtta cagaatggct aaatatacca gaagataaga tatgggtgac tgtatatgaa
    13081 gaagatgatg attcttatga tatatgggca aaagaaatga atttcccaga agaaagaatg
    13141 gttagacttg gaaaagatga taatttctgg gaaataggta caggtccttg tggtccttgt
    13201 tcagaaatat attttgatag aggagaagaa tatggatgtg acaatccaga ctgtaaacca
    13261 ggctgtgatt gtgataggta tttagaattt tggaaccatg tatttactca atttgataga
    13321 gatgaagaag gaaattatag tctattagaa aacaagaata tagatacagg tatgggtctt
    13381 gaaagaatgg gatgtattat gcaaggcgta gacactatct ttgaagttga tacaattaaa
    13441 tctatactag aagcagttga gaagttaaca ggagtaaaat atggagagaa tccaaaaaat
    13501 gatatatcaa taagaataat aactgaccac ataagagctg taacattctt agttagtgat
    13561 ggagtgcttc catcaaatga aggaagagga tatgttctta gaagactcct tagacgtgct
    13621 gctcgtcatg gtaagttatt aggagtaaaa gaattatttt tacagaaact aatagatgaa
    13681 gttataaaag taaatgataa agcatatcca gttttagttg aaaaagaaag ttatattaaa
    13741 aaagtagttg gaatagaaga agaaaaattc aatgaaacta tagaccaagg aacagaaata
    13801 ttgaactctt atatagaagt attaaaaaat gaaggaaaga ctgtcctaag tggtcaagaa
    13861 gcttttaaac tatatgatac ttatggcttc cctatagact taactaaaga aatattagaa
    13921 gaagaacatc tatctgtaga tgaagaagct tttaatgaag aaatggaaaa gcaaaaagaa
    13981 agagctagaa atgctagagg aaatatggat ggagaaagtt ggaaggaaga ccctctatca
    14041 aaattagaat ctacagttga cagtacattt aatgggtata gtgaaattta tggtgaagga
    14101 actattgaag ctatagttaa agatgatgag ctagttcaaa gtgcagaaga aggcgataag
    14161 gtatctatag tactagataa cactactttc tatcctgaag gtggaggaca agttggagat
    14221 tgtggattga taactaatga aaacttagtt ttagaagtat taaatactaa aaaaggtgct
    14281 aataatagta taaaacatat aggtataata aaatcaggaa gaataagtaa tggggacaaa
    14341 gtaaagacac tcgtagatag agaaactaga atgtcagctg caagaaatca cagtgctaca
    14401 catttacttc ataaggcatt aagagaagta ttaggagaac atgttaatca agctggttct
    14461 ttagtaactc cagaaagact tagatttgat attactcact ttgaagcaat atcaaatgaa
    14521 gaattaaaag taatagaaga aaaagtaaat aatgttattt tatcatcttt agacataaaa
    14581 tgtgatatta tgaatataaa ggaagctaaa gaaaaaggag ctacagcact atttggtgaa
    14641 aaatatggtg atgaggtaag agtagtttct atgggagatt attctacaga actttgtgga
    14701 ggaactcact tgacaaatac ttctcaagta ggaatgttca aaatattatc tgaaggtgga
    14761 gtagcagcag gtgtaagaag aatagaagca ataacaggaa aagctgttta tgaataccta
    14821 aaagaaagag atggaattat ctctgaagtt tgtgtaaact taaaatctaa agaagataat
    14881 ttaattcaaa gaataagttc tttattagaa gaaaataaaa acttatctaa agaattgcat
    14941 gatatgaaag ctaagatgag tttacaatca gttgattcta tttttgattc taaagttgaa
    15001 gtaaatggag ttaatttgat aactaataaa tttgaaggta tggatatgga tacactaaga
    15061 gaaacagcag ataatctaag agataagctt ggttctggtg tagtagtact tgcaaatgta
    15121 gtagatgata aagttaactt tgtagttact gccactaaag atgtattaga taagggaata
    15181 cattcaggta atattgttag agaagttgca aaaatagctg gaggaaaagg tggaggaaga
    15241 cctaatatgg cacaagcagg agctagtgat gtatctaagg ttgaccaagc tttaagctat
    15301 gcaagtgagg ttattaaaac acaagttaaa taacattcac tcaaggaggt ttttagtttg
    15361 gaaaaagatt tagactacac tatgaaattt gaaggaatac cagaagatag gatgagtgta
    15421 ggagatacta ttgattttgt atataaagca ttagttgaaa aaggatataa ccctataaat
    15481 caaattatag ggtatctcct ttctggtgat tctagctata taactagtca taaaaatgca
    15541 agagctataa ttaaaaaatt tgaaagagat gagatacttg aagaagtaat cacgcactat
    15601 ctgaatagaa agtaggtcaa ataaatgaga aaaaagatta tgtcgttgtt tgcaatagtc
    15661 attatgattt ttgtatcaac tgctagctat ggttttgctg aagaaaaagg caaggtcata
    15721 tttattgata tgaataggac caatcttgga agtatgatga atattccaat cttgaaaaat
    15781 gagatagaaa agagaggcta tgtagctctc atgaatataa gaggggatca aggtactgat
    15841 gatagaaggt cttatgcaag tatgggtgca ggaacaagag ctagtgtaac tactgaagac
    15901 tatatcaatt ttcaaaaagt agaccaagat actgctaaag cttttgaagt cgcgacaggt
    15961 caaaaagcta aagaaataaa tgatttaggc attaatagat ctataaatga aaatcttgag
    16021 tatggagaat atggagcaaa tttagggtca ttaggaaaaa ctttatcaga aaatggttta
    16081 aagacatctg taattggaaa tgctgatata attgaaaata atcaattagt taaaaacaga
    16141 aacttatgtt tagtagctat ggaccaatat ggaaggattg attcaggtaa tattgataat
    16201 attaataaga aagattctaa aatgccattt ggaatatcta cagattatga taaattacta
    16261 gatgaaacta aaaaatccta taaaaatagt gatgctatat ttgtagaact tggagataca
    16321 tatagattag atatgtatag attaaatcta aatgataata cttatgatac aatgaagaaa
    16381 aatattgtaa aaaatataaa taaatatctt gaacaagtat tttctatggt tggagaaaat
    16441 gacactgttt atatagcaag tgcatttcca agtgacctag attataagaa taagagaaga
    16501 ctttctccaa taataaaatt tagtggagaa gggaaaggtc tactggaatc tgccacaaca
    16561 agaaaggctg gaattgttgc caatttagat gttggagtag atatactagg tacatttggt
    16621 cttgaaaata aagagatggt aggtagaagt tatactttag taaataaaga ggacaatatt
    16681 agctatctaa aatcacttta tgaaaggata gtgtctatat cttcaataag gtcaactatt
    16741 ataaatggct ttgtaggcgt aatatctgct tcttgggtaa ttgccatgtt tgcgattttc
    16801 tttagaaatt atatgccaca taaagagcgt gtattcaaag tacttaagga gtttataaaa
    16861 ttaggcataa taatgccact atcatttatg ttagctcctt tgatgaattt taaaactcca
    16921 atttcaatat ctttgggaat atttgcaact acgctaatat tatacttgtt aggtagaaaa
    16981 ttatttaagg acaatgatat aaagcacatg ggattttttg caacacttac tattttagtc
    17041 atagttatag attctgtatt tggaacatac ttaatgcaaa ataacataat gagttatgat
    17101 gctgtcatag gagctaggta ctatggtgta ggaaatgaat atgaaggtgt aactatagca
    17161 agtgcagtat ttggcttagc agtattatta cattataaga aggtatctaa attacttgtt
    17221 gtaatttttt cattaataat acttataact agtgcatatc cttcaatggg tgcaaatgtt
    17281 ggtggagcta tatcagaatg tgtagcatat ttgttgttta taatgcttat atatgacatt
    17341 aaaatagatt ttaagaaagc tgtattatta gctgtttcag ctgtagttgt cgtttttgca
    17401 tttgcagctt tagatatact ttcaggtagt gaatctcatt taggtatgtt tgtacagaga
    17461 attttcctaa atggaccagg agagatatta caaacatttg gaagaaaaat tcaaatgaat
    17521 atgcaactag cacagacaag tgtatgggta aatattttac ttgtaggaat tggagtaatt
    17581 gcagtgctta tatttaaacc atctagacac tttagaaaga taatgaataa ttttccaatc
    17641 ttgtttaaag ggtttattgc atctatggtt ggttgtataa ttactctttt agttaatgat
    17701 tctggaatag ttgctgcatc tacagcatct atatatatac ttataccact aataataata
    17761 agtataaata tgataatatt tgaaaaaaat aactaaaaaa atttttactg ttcatatagg
    17821 atttaaataa aaaatgcaat atgattatta atgtaaactg gatgcttaca taaaaaatca
    17881 tattgcaatt tttatgattg attatgtaaa attctatgaa gcagtaagcc tgtgacctta
    17941 gttatgagag gttcagaata tgtatttaat ctatagaata taaatagata aaggaggaac
    18001 tatgttagat ggaagaataa tgggtcttga tgttggtgat aaaactattg gagttgctgt
    18061 gagtgatttg atgggattaa ctgcacaagg tgtaaaaaca ataaaaagag ttggcaagaa
    18121 aaaagatatt gaagagctaa aagcaataat aaaagaaaaa caagtaaata aaattgtatc
    18181 aggattacca aaaaatatga atggaacttt aggtccacaa ggagaaaaag taatcaagtt
    18241 ttgtgaattg gtaaaagcag aaacaggtat tgatgtagag ttttgggatg agaggctttc
    18301 tacagtagcg gcagagaggt ctttattaga agcagatgtt agtagacaaa aaagaaaaaa
    18361 ggttatagat atgttggcag cagtaataat tttgcaagga tatttagatt ttaaaataaa
    18421 ttcataaaag aactataaaa ttgaatactt atgatatata atataaataa taaaaataaa
    18481 aataaatttg aggtgaatga cattatggaa gaaaatataa taaatttaat agacgaaaat
    18541 ggcgttgaaa gccaatttga aataatatta acattagaag cagaaggaaa agagtatgca
    18601 atattaatgc ctttagatga tgaagaagct gaagaagctt taatctttag aattgatgag
    18661 gatgaagaag gtgaaatctt aatacctttg gaaagtgatg aagagtatga aactgttgta
    18721 gctgtgtata ctgctataat ggaagaagaa ggtttaaatt acgacgaaga tgaatcaaat
    18781 ggacttaact agataagtaa ttaagtttgt atataaaaat cagatattta ttatatttgc
    18841 tttaattaaa ccttattaat tgtaaaagca tttatagtat atatctgttt ttttgttttt
    18901 tattaaaaat accaaaaaat ttatgaattt tggtgaataa tatatctttt atgggtatat
    18961 atttatatta tgagaataaa ttaaatttat ttaaatgtct tgacaaaaaa tgaattatca
    19021 attacaatat tatttaataa agattatcaa ttgaaaatag taagggtgat gttatggcaa
    19081 atacaatgga tttattaaaa gacaagttaa aagaaactgg ttttaagatt acgccacaaa
    19141 gaagagcaat agttgaaata ttgcttaaac atgaccattc acatctaagt agtgaggaaa
    19201 tctatgattt agttagggtt gactgtcctg aaataggctt agctactgta tatagaacaa
    19261 tgcagctatt ggatgaaata ggattaatat caaaactaaa cttagacgat ggatgtataa
    19321 gatatgagat tagtctacat aaagaagact gtcataatca tcatcatctt atttgtaaaa
    19381 attgtggaaa gataatggaa gctaaagaag accttttaga caatatagaa aaagagatac
    19441 aatcattgta caaatttaaa atacttgatc atgatgttaa attttatgga ctatgtgacg
    19501 agtgtaatgg agtaagcgat tcagaagaat aaacttctat tgcttaatgt cgatggaaga
    19561 atagtataga acttttatat tctatgctat tcttccattt tttattataa accatctata
    19621 aaatgcaaat atacagtttt caagatgctt atagttttaa tattgaaaat ataattaatc
    19681 ctataagtac aaaaatgaat gatttttcta tgaaattact aaatggtcct ccaacagtta
    19741 aaaaacctaa agaaattttc ttttcgctta gcggataaaa aattggtatt ccttgctttg
    19801 taaataaatc tcctaaaaac atatgacttg cataaccaat cgtaccataa aaagataaat
    19861 tattaatatt agtgattaat tctatctgtt ttagtaaaaa atatactatt atagttgcaa
    19921 atatgctatg agaaaaactt ctgtgcttta accagggaaa aattgacaac attagacaaa
    19981 aaattgtaaa atatatttca acttctatga tgtataagca tatagctaat agtataaata
    20041 ttaatgatag aaaaactttt cttagaaatg tgtgggttag ttttgcttca ataattataa
    20101 ttgcaacaaa tgtaattatt gcgcttaaat aaaaattatc atttatattt attgaaataa
    20161 agaaaataat aatattaaca gcatatataa aaattctata tatgtatttg gatacattgt
    20221 tttttagaat gaaatttgag accatagaat tagatttatc caaatcgggt agtacagcaa
    20281 aagtagctga aactaaaata tcaactaaag atataggaat tttaaatatt aaagatgttt
    20341 ggatagctgt taagattcct attgcacaat gagttttacc acgcataaat cctcctaaat
    20401 tatccgatat taaatcatat aatatttaga ggatatcaaa ataaaatagg tttgtcaaaa
    20461 taatacgtta aatgtatttt aaatatagct taaaatatga tacgaattat ggtacaataa
    20521 gaataactgg tagcgagtgc gtgatttgat attatttatg attgtagaac gtgttaaaaa
    20581 attaaataga cagaaggaga tgatgcaatt gttcaaaaag aacaccaaca agataaaggt
    20641 aatggccttg ggtggactta acgaagttgg aaaaaatatg actgttgttg agtataaaga
    20701 tgaaataatt gttatagatg ctggacttag ttttccagag gatgaaatgt taggagtaga
    20761 tatagttata ccagatataa cttatttagt aaaaaatagg gataaaataa agggtatatt
    20821 tattacacat ggacatgaag accatatagg agctcttcca tatatattaa agaaaataaa
    20881 tgtacctgta tatggggcaa gacttagtat aggtcttata caagtaaaac ttaaagaaca
    20941 taaaatgaat aatgttaagt taaatgttat aggtcctaga caagttataa agcttgataa
    21001 tatggaagta gagtttttga aaaataacca tagtatacca gatgcttact ctattgcaat
    21061 acacacagac caaggtataa tataccacac aggagatttt aagattgact tgactcctat
    21121 tgatggagat gttatggata tgcatagaat atgtgagttg agcaaaaaag gagttctttt
    21181 aatgctggca gatagtacta atgcagaaaa accaggattt actatgtctg aaaagactgt
    21241 tggggtaggt cttgacgaat tatttgcaaa aggaaatggc agaagaatta tagttgctac
    21301 ctttgcatcc aacatacaca gattacaaca aataataaat actgctgaaa agtttaatag
    21361 aaaagttgct atatcaggtc gctcaatggt caatgtagtt ggagttgcaa aagaattagg
    21421 atacctagat atttctgatg acatgcttat agatttaaat gatatatgta agtatgaaga
    21481 tagtgaatta gtgataataa caactggttc acaaggtgaa cctatgtcag ctcttgctag
    21541 aatggctttt tcagagcaca aaaaagtaga aataaagagt ggcgatttag ttataatatc
    21601 tgctcatcca ataccaggaa atgaaaaatt aatatcaaga gtaatcaatt tcttatttga
    21661 aaaaggagca gaagtagtat acagtgatat agctgatata cacgtttctg gacatgcctg
    21721 tcaagaagaa ttaaaactta tacatgcatt agttagacca aagtttttta tgccagctca
    21781 tggtgaatat agaatgttaa aaagacatgc tgaaatagct gaacagcttg gtatggataa
    21841 agaaaatatt tttgtcatgc aaacaggaga tgttttagaa ttagataaaa actctgctaa
    21901 ggtagcaaac cgtatacaaa caggaaacat attagttgat ggtctaggag taggcgatgt
    21961 aggtaatata gttttaaggg atagaaagca tttatcagaa gatggtctta tgatagttgt
    22021 agttactatt tccaaggatg agggtaaggt cttagcaggt ccagatataa tttccagagg
    22081 attcgtatat gtaagagagt cagaagattt aatggatgga gcaaaggata taattaagaa
    22141 tgttctaaat gaatgtgaag agaaaaatat taaagaatgg gcttatttaa agaataatat
    22201 taaagaaaac ctaaaagaat atttatatca aaaaactaaa cgaaatccaa tgatacttcc
    22261 tatcataatg gaagtataat ataaaaaaac tataaatttg aaaattagac ctcttgtaat
    22321 ttttaaagta atatcaaaaa tccaattgat attatcttaa gaattacaag aggtcttttt
    22381 aatactcatt taaaaaaaat atatattaca taaaatacca attataacaa tcttttatta
    22441 gtaaaaacta cttatatata aattttactg ataggaggat tcttatggat gatgtttcaa
    22501 gacaaaatgc tataaaagct ttaaaacaaa caaaaatgga aatagcagga gagtatggaa
    22561 tgaattatga agatgctttt gaaataatag aaaatgcgtc taataaaggt gttttagaag
    22621 gttactttaa aaagcttgaa aagaaaaaaa atttaggtca aggtatatct aggcatttag
    22681 aataaaaaaa gttaaatgtg agaaaataag tttaaataaa ctttaagagg tgacatcgat
    22741 gaaaaaatta gctagtactg ctttggctat attaattgcg cttactcctt taagcttttc
    22801 ttttgcaaat aataaggaaa atgctgatgc caaccaacta aatatatctt caaaatcagc
    22861 aattttgatg gatgttggta gtggacaaat tttatatgaa aaggatgctc ataaaaagct
    22921 tcctcctgct agtgtaacta aggtaatgac tatgctactt atagtagaag ctttggattc
    22981 aggcaagatt aagcttgatg atgaagttca agtaagtgaa actgcatcta gtatgggtgg
    23041 tagtcaaata ttcctagagc caggagaaac tcaaaaggtt gatacattat taaaaggtat
    23101 agctgttgct tctgcaaatg atgcttgtgt tgctatggct gaacacttag ctggaagtgt
    23161 tgaaggattt gttgatagaa tgaatgctaa agcaaaagag ttaaatatga atgatactca
    23221 ttttgcaaat acaaatggac ttccagttgc aaaccactat acatctgctc atgatatagc
    23281 tttaatgtct agagaactat taaaacacga aatgataagt aaatacttaa ctacatggat
    23341 ggataaggtt gtagtaggga agaaacaagt tacagtaggt cttgcaaata ctaataaact
    23401 tataaagcat tatcaaggag caactggtgt taaaactggt tttactcaag aagcaaaata
    23461 ctgtttatca gcatctgcaa agagaggtaa tactcatcta gttgctgcaa ctttaggagc
    23521 agagacttct ccagaaagat ttaatgatgc ctctagcctt ttaaactatg gatttgctaa
    23581 ttatgaaagt gttaagttat gtagtaaagg tgataatata gcaacactta cattggataa
    23641 ggctgatgaa aacaaagtta aattggttgc taaagaagat ttaaatgctc ttattaaaaa
    23701 aggaagttct aaagaatttg agaagaaaat tgaaatagta aaaaatccta agatgccaat
    23761 taaaaaggga actgttttag gaaaaataaa gatatgtaaa gataaaaaag taataggcga
    23821 agtggaattg ataaatacta aggatataaa taaggcaagc tatttacaaa tgcttcaaag
    23881 aataattgat aatatgatat aaataattat cagatgaaat ataattataa tacacaaaga
    23941 tatgattata atacacataa aaaataccct tatgtaatta tttgccatag gggtattttt
    24001 atttaccata tagaagtatt ttataatctt atgattttta tagaaatata gaaaagttta
    24061 tgtatcttaa aatatcattt gatttaatat catatatagg gttgtttaac aattatctaa
    24121 gtaaaggcat actaccagta agtttattca ctttcttttt attcccataa attgctatcc
    24181 caaaatactg taactcatct tctggaacac tagcaatctt ttgtgtaaat tcttcataaa
    24241 gattgcaact ttgtgctaca tctgaaaaat ctacaacagt taaatcgtta aattccaaag
    24301 tatataattt tttacgtaaa tcttttatta tttctttgtt tccttttaaa ataggtacag
    24361 gaatgtcaat aattcctaag tggctattac ctgttttgtc aattacatct gggccaacag
    24421 tttctggggc atgttttcct agtgtaattc ccattatagc tgctgtattg gatataattc
    24481 ccattggtaa attttcgtct attaccatga cacattttga atttttagtt tccattttta
    24541 tttctccttt ttaccttatt taaagttgtt tgaatcatca tttataaata tattttgata
    24601 ctgctttggt gtaagaccaa taaaattctt aaaaaagttt gtaaagtgac tttggtctac
    24661 aaatcctgtt tgtatcgcag catctatagg ttctacgcct ttttctaaaa gctttttagc
    24721 tttgtttacc cgtatatttt ctaggtatct atatggtgta atacctttta atttcgtaaa
    24781 attacgaagt agagagtact tattcatacc tgctatagtg cttaattcgt caagtactat
    24841 atgttcagca taattatttt ctagataatc acatactgct tgaatttcta tatttgtatt
    24901 ttccaagtta gattgcaaat ttggctctgt atgttcttca attaattgtt caattaggaa
    24961 gaaaaaaagc tcttcctttt taaaatccaa ttcttcttcc atgattatat agtgtaattc
    25021 ttgtagtaga ggtactagct cactgcgaaa tataacaggt tgattaaatt ttggtaaata
    25081 atttttacct gtaatttcaa agacggtttt tttcattatt tctggcttaa tattaataca
    25141 tctgtaatca agtactttat catctatttg ttcacaagta tgactatcaa aagggttaaa
    25201 aagtagcaaa tctccagtac ttgtggtata ctctttattt ttacaagata aatatctttg
    25261 acctttttct ataaaaccaa ttacataatg ttcatgaaaa tggtttggga atttttgcat
    25321 tatgccttta aatctatatg cttcaatttt taattcccta tcaaagcata ctgtgcgttc
    25381 ttcttgtctc acttaggtaa cctcctttca tgttatacaa agtgtaacac atattttttt
    25441 atacttcttg tataatattg actaagagac aataatgtca attcatgtca atataatatt
    25501 acacttacag taggtgtaat tattatattt tcacaatgaa tatgatataa taataaaagt
    25561 tataaaataa aaagcaaatt ttaattttat gcctagaagt taaaattatt ataatgtaaa
    25621 ataattttga ttataagcta tttaattgtt ataataatat taaaaattta atttaggaga
    25681 tgtacattaa tgttaagtat gaatatagga gaaatagtag caagtttagt tggtatagct
    25741 atggcaatat caatacatga gtttggccat gcttattcag ctcacttatt gggagatgat
    25801 acagctaaag cttatggtag aatgacatta aatcctgtaa gacatgttga cccaataggt
    25861 cttttggcta tgtttatcct taagataggg tgggcaaagc cagtaccagt aaatccaaat
    25921 aactttaaaa actataagat aggaaacctg atagtttcac ttgcaggcgt tttttgtaat
    25981 gttttgactg ctatactgtg tgtaattatc aataagtatg tgcatatgta cgctataaat
    26041 acaatagctg gatatgtgct tttatatagt atgggatttg cagcttttaa cttacttccg
    26101 atacctccac ttgatggatg gggagttata tctacatttg taccatataa atggaatgaa
    26161 tatttgtata aatttgaaag tatgagttat ataattttat taattgcttt atttactggt
    26221 atatatcaaa taatactatc accaatatat acagtgttgc taaaaatagt atatttattt
    26281 gtttaatata ataaacagat taaagaggga ttgatatgaa gtataatata caattacaag
    26341 tctatgaggg accattagat ttactttatg atttaataac aaagcataaa atagacataa
    26401 aggatatatc tataatagat ataactaagc agtatctaaa ttatttgaaa atgctagata
    26461 aaatggattt agagataact agtgaattta taactatggc atcaaaactt ttagagataa
    26521 agtctaaata tctgctttat aagcaaaaag atgaagaaga agacccaaga atagaactta
    26581 tggaaaagtt agaagaatat agaaagttta aggtagcatc acaagatata aaagaaaata
    26641 taacttatgt taatgagaga ttttatagaa ataaagaaga gataattatt gatgacaatg
    26701 tggatttaga agatatatca attgaagcaa taaaaaatat acttccatac atatttaaag
    26761 ttaaaacatc acagatagaa aatgctaatg atgagaaact agataaaata gtaaggaaga
    26821 aaataatatc tgtagaagaa aaaatattat atataagaga tattataaaa gataagattg
    26881 aagttacttt tacaaatatt ataaaatcat atgaaaatga tgaaataata gctacttttt
    26941 tatctatatt agagcttata aaagaaaaag aaatagtcgt agtacaagat atattttttg
    27001 atgatattct aataagaaaa agttcggagt gttaaaatga aaagagaaga tataaagtat
    27061 attatagaat ctgttatgtt tgcatatggt gaaccaataa gtataaaaga attaaactat
    27121 ataataaata aagaactttc atcaaaagaa atagagatta tgttaaattt acttattgag
    27181 gagtacagag aacaaaatag aggtattcaa ataatcaagt tagagaataa gtaccaaatg
    27241 tgtacaaata aagactatgc agaatatata aagaagatta ttgaaccaaa gaaaaagaaa
    27301 agtttgagcc aagctacatt agaaactctt actataattg cctacaaaca accaataaca
    27361 aaggtagaaa tagaggatat aagaggagta aaatgtgata aggtattaca aactttgttt
    27421 gaaaatgaac ttataagaga agctggtaga ttgaataaga tagggaaacc aataatttat
    27481 aaaacaacag atgaattttt aaagttgttg aatatagaaa gtcttgaaga attgccacct
    27541 atagaaaatt atcaagaagt ggtaacaagt gaataaaaat ataaaatcac aataatttag
    27601 tatgtaattt t
//
LOCUS       DACXMZ010000026        26904 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.026, whole genome shotgun sequence.
ACCESSION   DACXMZ010000026 DACXMZ010000000
VERSION     DACXMZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.026"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..757
                     /locus_tag="JD016_04435"
     CDS             <1..757
                     /locus_tag="JD016_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HAU4998876.1"
                     /translation="KKTQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNIN
                     ISKSGYSSINAKQVPYNLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGD
                     SIDLNVDLDTIMNNKDAKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKI
                     SFKNNKNISIINGFLRYPETKNVFIGHTGICIKTKNGILFLEKYGATSPYQVTKFKNK
                     KDVKNYMFNRLKMSEGEIELPDPIIMENDKLMK"
     gene            complement(1070..1849)
                     /gene="hisJ"
                     /locus_tag="JD016_04440"
     CDS             complement(1070..1849)
                     /gene="hisJ"
                     /locus_tag="JD016_04440"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HAU4998877.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(2395..2745)
                     /locus_tag="JD016_04445"
     CDS             complement(2395..2745)
                     /locus_tag="JD016_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HAU4998878.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(2761..3177)
                     /locus_tag="JD016_04450"
     CDS             complement(2761..3177)
                     /locus_tag="JD016_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HAU4998879.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(3196..3387)
                     /locus_tag="JD016_04455"
     CDS             complement(3196..3387)
                     /locus_tag="JD016_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HAU4998880.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(3538..4353)
                     /locus_tag="JD016_04460"
     CDS             complement(3538..4353)
                     /locus_tag="JD016_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAU4998881.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNIEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(4400..5338)
                     /locus_tag="JD016_04465"
     CDS             complement(4400..5338)
                     /locus_tag="JD016_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAU4998882.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIS
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(5335..6207)
                     /locus_tag="JD016_04470"
     CDS             complement(5335..6207)
                     /locus_tag="JD016_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAU4998883.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(6232..8160)
                     /locus_tag="JD016_04475"
     CDS             complement(6232..8160)
                     /locus_tag="JD016_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HAU4998884.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGIIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLVLISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(8272..8649)
                     /gene="gcvH"
                     /locus_tag="JD016_04480"
     CDS             complement(8272..8649)
                     /gene="gcvH"
                     /locus_tag="JD016_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAU4998885.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(8736..10862)
                     /gene="cdhC"
                     /locus_tag="JD016_04485"
     CDS             complement(8736..10862)
                     /gene="cdhC"
                     /locus_tag="JD016_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HAU4998886.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(10919..11725)
                     /locus_tag="JD016_04490"
     CDS             complement(10919..11725)
                     /locus_tag="JD016_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HAU4998887.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(11775..13142)
                     /locus_tag="JD016_04495"
     CDS             complement(11775..13142)
                     /locus_tag="JD016_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HAU4998888.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(13173..14117)
                     /locus_tag="JD016_04500"
     CDS             complement(13173..14117)
                     /locus_tag="JD016_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HAU4998889.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(14137..14907)
                     /locus_tag="JD016_04505"
     CDS             complement(14137..14907)
                     /locus_tag="JD016_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAU4998890.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(14963..16348)
                     /gene="lpdA"
                     /locus_tag="JD016_04510"
     CDS             complement(14963..16348)
                     /gene="lpdA"
                     /locus_tag="JD016_04510"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAU4998891.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(16392..17273)
                     /locus_tag="JD016_04515"
     CDS             complement(16392..17273)
                     /locus_tag="JD016_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydrofolate reductase"
                     /protein_id="HAU4998892.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKLLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(17309..17983)
                     /locus_tag="JD016_04520"
     CDS             complement(17309..17983)
                     /locus_tag="JD016_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydrofolate reductase"
                     /protein_id="HAU4998893.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(18020..18883)
                     /locus_tag="JD016_04525"
     CDS             complement(18020..18883)
                     /locus_tag="JD016_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HAU4998894.1"
                     /translation="MSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVRVGA
                     RSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCFRPL
                     PKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNVDLK
                     GSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRAKMV
                     DESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSILAKH
                     VVKACKLQNNK"
     gene            complement(18984..19616)
                     /locus_tag="JD016_04530"
     CDS             complement(18984..19616)
                     /locus_tag="JD016_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HAU4998895.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(19721..21397)
                     /locus_tag="JD016_04535"
     CDS             complement(19721..21397)
                     /locus_tag="JD016_04535"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAU4998896.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(21646..22425)
                     /locus_tag="JD016_04540"
     CDS             complement(21646..22425)
                     /locus_tag="JD016_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAU4998897.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(22735..24654)
                     /gene="cooS"
                     /locus_tag="JD016_04545"
     CDS             complement(22735..24654)
                     /gene="cooS"
                     /locus_tag="JD016_04545"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HAU4998898.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(25261..26172)
                     /locus_tag="JD016_04550"
     CDS             complement(25261..26172)
                     /locus_tag="JD016_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HAU4998899.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(26288..26866)
                     /locus_tag="JD016_04555"
     CDS             complement(26288..26866)
                     /locus_tag="JD016_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAU4998900.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRAIDEIPE"
BASE COUNT         8015 a         5444 c         3056 g        10389 t
ORIGIN      
        1 taaaaaaact caaaatagtg taaaggaaat cctaaaagaa aataaaatta atgaaaaaga
       61 catagataca tttattaaag cagtcaacaa ttataataaa ttgcaagtta aaatacttca
      121 aaataatata aatatatcta aatctggata ttcttcaatc aatgcaaaac aagttccata
      181 taacctagaa aaattgcaag ataattgggt gaaaaaattc cctgattata tggatgtaaa
      241 ttgtagaata actgctttta gactttttaa agattttata aattcaaata agaaatttac
      301 tggagactct attgatttga atgtcgactt ggacactata atgaataata aagatgctaa
      361 atttagcacc aaagatgtag aaaaatttat caacttcttt tctgcaatac cagctaaaga
      421 cacagatgat acaatcaaaa ttgcagaaca aataaaaaat gaatggaaaa aaagaaaaat
      481 atcttttaaa aataataaaa atatatctat tataaatgga tttcttcgtt atccagaaac
      541 taaaaacgta tttataggtc atacaggcat atgtattaaa actaaaaatg gcattctatt
      601 tttagaaaaa tacggtgcta catcacctta tcaagttact aaatttaaaa ataaaaaaga
      661 tgtaaaaaat tatatgttta atagattgaa gatgtcagaa ggtgaaattg aattacctga
      721 ccctataata atggaaaatg ataaacttat gaaataataa ctttaaacgc aaaatagtat
      781 ttataattac taagtcaata taatagagaa ggtctataga tttgtgtaaa tacaagctta
      841 tctacaatct tatataagaa tgtttatata aataagatag attaattcta agtcctatta
      901 ttctattgaa ttttattatt attttattta gattctattg ttattttact tagaacttat
      961 tattatttta tttaagttct attactattt tcaatcatat aaatttacaa aagactgtac
     1021 ataaatttat tttatacaaa cttatctaca gtcttttgtg tgaatatatc taaataatat
     1081 cgtatttatt tatttcctct gcacagtgag aatcagaacc taaaactaat ggaatatcgt
     1141 atttttttac caactctaat aagtatccat ctatataagg ctctttgcag tactcttttc
     1201 tatttccaga aacattataa tcaagttcat aacctttttc tttaattaac tttattaact
     1261 cttcaagaat tgtatttcct ttataatcgt atggaaatac ctgattaaac tttctaacta
     1321 aatttaaatg acctattctt tttggtttat gtatacccaa atctgaattt acagctttta
     1381 tcaatgtttc ataatactta ttataaacag cttcaatact tcctaatttg tcgattaaat
     1441 tttgaaactc ctctacactg taatctacac aataataatc atcatcaatc ttaattatat
     1501 gtactgataa aagagaatcc tctaattcct ttccatattt atttaaattc tcctttatcc
     1561 cttcttcgta tccttctata aaatccactt ccgacccaac atttatcttg attttaccct
     1621 catacttttc tttcaacttc ttgacatcat taaaatactt tggtaaatct tcaatactca
     1681 tacaactatc attttcagga gcagggtctc taaatccatt tgcatacgga aaatgttctg
     1741 taaatgtcat ttctgttatt ccaacatcca ttgctctttg tacatatttt tcaaaatcat
     1801 ccttacttcc atgaggacaa taaggtgaat gtatatgacc atctttcata aataattcac
     1861 tcctttttta aagaaaaaat ctaatataaa tgcataaatt ttttaacttc aaaataatat
     1921 ttttaatcat gctaattttt attatatcta atattagagt aattttaaac tgccttttaa
     1981 gtatcttatc attagtacta ttttcaactt aacattaata ttatttttta acttaacatt
     2041 aatactatct ttaaatatta atactcaata tataattact taacataaaa taactcaaat
     2101 tgcttgatga ggcttttgca atatttaaag aatatataat tatttaacat taaataatcc
     2161 aatcttgtaa taaaatttac tcattaaagt atattacaga aaatacctca aaataaactt
     2221 tttagttcat gagatactct tttttacatg aaattagata tatttttatc atttaaacaa
     2281 tgaaagggag gcttatatcc atctttagac aaccccttag ataaaatttt taataattaa
     2341 acatcacaaa atataaaaat cgcttatatc aaacgtataa gcgattaatt atatttacat
     2401 atgtttttta gcctctaaca acattttatt agcttcttcc atatactcta tagccttttc
     2461 aatactcttt gctgtttctt cttttccaat agctctagcc ttttcaaccc actctctaaa
     2521 tccttcttca tgagtttcat tgtgatttat ccaatgtact aaaagtattt ttaaagtttt
     2581 ttcatcctta gactctgatg attcctcatg actatgttcg tggtcgtggt cgtgagcatg
     2641 gtcatgtgga tgttcatgac atatatctcc atgagtatgt tcgtggcagt ggtcatggtc
     2701 atgatgtcca tgatgatatc ttacaatctc tttatcaaaa atcattagtt attccccctg
     2761 ttattttata ttaaactctt tttttataag gtttacagaa tcttttacaa gtagtgctaa
     2821 tggtttacct tctaatccaa ctaactccac attttctggc ataataggta aaagtatctt
     2881 aagtgcctca ctgtctgcaa tagcttcact tatattacaa gtaacttctc ccatcataga
     2941 attaggtatt attacagaaa taggtgcaac tattatattt gcttttttag ctgatactac
     3001 tatagcattt tcccctgtag ctcctttatt agcatgagct ttcatcatag aactagttgc
     3061 tatagaatta gtccctagtg cataaatttc tacatatgta ggtaactctt ctctcaaaga
     3121 agaaactatc tgggctccaa tacctccacc cattccatct atcactgcta ttatcatatg
     3181 aaattcccct attaattaat tttcttgtat tattatctta tggtccatca atcttatttc
     3241 tttaattatc ccatctatta tcttttgctc tcctaataaa tccgttaaat aaatctttcc
     3301 atcacaaggg tctatattaa ccacattttc cataaccttt tctaactcat tattactttt
     3361 acaaatgaaa gctgatgatt cacacatata aatctcctcc taatatgatt tttattaaaa
     3421 atcaaaaaaa gattccctta ggtatttcaa tacaccaaag aaaccttcat gatttcagtt
     3481 tttgtatatt attatacctt aagtatatca ctttaattta tgtaaagtca attaaagtta
     3541 acgcttttta ttatactctt cattcttgta tttttcatat agttctttaa ctaattgctt
     3601 ctcataatca gtcaaatcgc cctccacaaa ctcaatatta aattgttcag ctaatccctt
     3661 tactatgttt ttctgtagaa tatctatgtc tatttttctg ccaatttcat tttctattcc
     3721 acttgctttt ttagctgtaa atttcatagc tctttctttc aattctggtg tcttagtttt
     3781 tattaattta aacaattttt ctacatcaaa atccagtatt attgaaccat gttgtaataa
     3841 aacaccatct tttctacttt gagcacttcc aattaccttc ttattattaa tagtaacttc
     3901 atatgatgca tgtgcattaa aacatgcagc tgataaattt tccctactta ttctctcgcc
     3961 tctatttaaa ttatctgttt ctataccact tagattcaac cctttaacta gaccttcact
     4021 tatatatcta taagataggt tgatgctctt atcgattaaa ggattatctt ctcctataat
     4081 tatactatag gttagctcat tatcatgtaa tacagctctt ccacctgtaa ttcttctagt
     4141 atagtctata ttcatacatc tacacttatc taaatctatc tcatcttcca atttctgaaa
     4201 atatcctata ctcaaacaag ctggttccca tgtataaaat ctaagagttg gcttattatg
     4261 tcctttctta tatgcagtaa atatagcttc gtcaattgcc atattcattg ctccttcata
     4321 tgatttatta tgtataactc tccactgatt catgattatt acgcccccat ttttagtata
     4381 aatatatcct agaaatcctc tatagattta tttctcttat tgctaaatct atctcacttt
     4441 gatttaaagg tcttgaatag ttgtatatag tacttcctgg tctttcatta tagtatggtc
     4501 tattacaatc cttacaacct cttatttcaa aaggatagcc tttgtttatg tcttttaaaa
     4561 tatcattgtc aagctttata ctatttaagt atccattttt aaacttaaaa tactcttttg
     4621 gataaccttt atttatcata taagacatta actgtaccct tctataactt tctatgcttg
     4681 gttgacttat cttttccatt ttagttcctc taactggagt aaatgcaaat aaactaatcg
     4741 ttacatcatt ttcctttaaa taagtatata aattgtatat atcttcatga ctttcaccca
     4801 taccaactat tatatgtgta cttattttat tgggatatga ttttgacatt tcagttataa
     4861 attttaactt ttcatcatag ttatttcctt ttattttttc atatagttct ttatttgctg
     4921 catctattgc tattcctatt ttatcaactc ctgctgaaaa aaacttcttt atctgttcat
     4981 cattttctaa ttttgcagat agtgaaattg gcatgtctat cttaccactt atatagttta
     5041 taaagtctaa aacagagtta tgtgcttctt cacttgccat tgattgaata catattctct
     5101 ttatattttt tcctttataa gcttttaaag catctaatat ttcttctttt gaaaactctg
     5161 gccaaattac tcttgataat ttatcttttc tagtactact ttctattgat tgagaacaaa
     5221 aactgcattt atttatgcat ttttctccaa tcattatata tgctgtggta ggaggtatat
     5281 cgcttttttt gtttaatatc ccaagttcta ttgctgtccc aactgatagt ctaatcataa
     5341 aacgcaacac tccttacact catttattgt catattcatt tctatagcct tattctttgc
     5401 agaccttgat ggtaatacta ttctattgac tccacacatt atagataatt ggtctagctc
     5461 ttttctatac actcctcttg gtctcataca acctagatta atttcagtat ctggtaaatt
     5521 aattctagct tcacataaaa tatctgctac cccttctaac tcaggaggtt ctacattttc
     5581 atactcagtc ccttttgttg gaataaggac tataaatgta agcttattag gaggattttt
     5641 ttgtaggtat tctataattt catattcacc ctttatttgt cctcctttta aaccaataca
     5701 tatatgtggt gctacttctg tatgttcttg tattgtatta tagacttcaa tataatcttc
     5761 tttactcttt ttcatcttat aaacttctct tattgtctca tcatcaaaca ctaaatcaaa
     5821 agaaactata tctaaatact tacacaactc tacaatgcta tctttatcca taagacctaa
     5881 atgagcattc aatctatatc cttgctcttt taagttttta atggtattaa tatgagtatt
     5941 tatagggaca tctccctcat aactacaacc gccgctcaat agaaagctag ttatattcct
     6001 tgattgtact ttttcctcat attcttctat tggcaccata ttgtttaagt agtgaccgtt
     6061 gcaatgggca catttcaaac tacattgatt gcttgtagta ctaagagcta gtgtctggtt
     6121 aggataagca aactctattt tatttccaaa attctttaat ttaatatcaa atgctatttt
     6181 cattttttca gatagattct tattcatagt ttattccttc ttaagtacat tttactcttc
     6241 tagtaatttc tttgctgttg ggaactgctc tatattcgta tgtggtaaga aacatgcaga
     6301 aataaattca tccatataac ttggatatac actaagttca acataagtta tttgattacc
     6361 tatttcttca agtttatttt ttgcatcttt acttatgaga actaagtaag aaccaacaag
     6421 tgaactattc cctatataag taaatttttc tctatcaata tcaggaagta accctataat
     6481 tattgagttt tctatgtcta ggttgtttcc tataccacct gctatataaa ccctatctaa
     6541 tatactaaaa tccataccta aactatctac aagagtgtat gcgcctgaat atatagctcc
     6601 tttagctctg atgaaattat ctatatcaac ttcattgact actatatcat tttctaaatc
     6661 aaattcttct ttaaatgcta atacatactc tcctatctca tgctcattaa atctaactct
     6721 cttattatct aaatccctgt atattttacc tcttctatct attatacctt tggtaatcat
     6781 ttgacagatt aaatcaatta taccagaacc acatatacca actggtgcac attcatcaat
     6841 aatctttaat gttggctcta aagtttcttt atcaataatt actttctcta ttgctcctgc
     6901 tgatgctctc attccacagc ttatccctcc accttcaaaa gcaggtcctg ctgaacatgc
     6961 acagctcatc atataatctt tatttccaaa tacaatctct ccattagttc ctaaatctat
     7021 aaataaaaca ttttcttcac ttgaccatat ccctgctgat aatacacctg ctgttatatc
     7081 tcctcctaca taacttgcaa cagatggagc taagtacaca tatgcactgt catttacaaa
     7141 aagaccaaca ttttcaccca taagttttgg agattttaaa aatggtggta tgtaaggttc
     7201 ttgtcttaag aagtctgtat acacacccaa gaataaactt gtcatagttg tattcccggc
     7261 agccactaat gtaactacat cttccttatc tattccattt ctttcatata ttgtttttaa
     7321 tagtggattt atagtttcct caactatagc tttatgaagt gtctctaatc catttttctt
     7381 agttgaatat ataattctat ttataacatc tgctccatat tttatctgtg catttccaga
     7441 agatgcttta tctactacct cttttgagta taggtctact aaacaaacaa ctactgaagt
     7501 tgtaccaata tctattgcaa ctccgtataa ggaattttct ttatttcctt gttctatatt
     7561 tatgattgta agtttctttt gtttttgaac atatgttatt gtaaccttaa aatcactctt
     7621 tctaaataca gttggcattt ttctaagtat atccaatcta aaatctattt cattatatcc
     7681 taggttattt ctaacatatc tttccaatct atctacatca cttatattat catctaaatt
     7741 tggttcttcc atttctatgt atttcttttt tatatttgtt ttaaattgaa ggttatgttc
     7801 ttcaattatt tttttagctc tttcaaaaat ttccctatcc tcttttttat tactaccttc
     7861 tatcttcata ccatgcatag aggatgatac tttagaagga acttcaattt ctatatctga
     7921 tattaccttt gtacaacaag ctagtatata accttgctcc cactcatcat ctgttatatg
     7981 tcttgttttt tctgtatcaa cttttccatt caataatttt actttacact tcccacaaga
     8041 cacgtttcca ttacaaggag catctatgaa tatatctgca tttcttgcaa cctctagtaa
     8101 tatatccccc tcattacaat aaacctcttt attgtttggt gtaaaactta cttttatcat
     8161 gttatccctc ccaatttgtt ctatatatga taaaaggaaa gtatagctaa atttgcgtgc
     8221 actcaaattt tactatactt tccccaatta taataatatc ttaatcttta attattctaa
     8281 tccagcctcg tattctttat cacttaataa cgtatcaagt tcagccacat cagctagttt
     8341 tacttttaat atccagtttt catatgggtc ttcatttata cattctggct catcttctaa
     8401 tttttcatta acttccaata cctcaccatt aacaggagat attaaatcag aagctacttt
     8461 agaagactca actactccaa aatctactcc cttagttaca gtatcttcta cttctggtgt
     8521 ttcaacaaac aatatttctc ctaattgatc ttgagcatag tctgttatac ctatatatac
     8581 aacatctcca tcaattactt ttacccactc atgatcttta gagtatttta attctggtaa
     8641 taatttcatt tcgatagacc cccaatatta tattattgat taaattactt aaatcatatt
     8701 aatattatat catattattt taaattttcc atcaattaca ttacactttc catagctaat
     8761 gctggatgac ctttttcttc taagaaagct aatactcctt cagaatcgtc agctatagtt
     8821 tcatcacaaa ccatatcaca gaagttttct attccagtca tttctttaac tgtagcattt
     8881 aatttatcag ctacataatc ttttaattct tttggcatcc aaactattct ttctggtcca
     8941 ccttctgcat aagcgaattt tttagaagat ataaaatgtc ttccgtgtcc cataaatcct
     9001 ggagtttgaa ctcctccacc tgtcatagaa gcaagttctc cgaaagtcat accaactgga
     9061 gtaacacttg caaattctct gttaactaca acaaatccat ttgcttctgg cattatacca
     9121 cagatacatt cgaagcatcc acaagaagtc attgggtctt ctagtataga gtataatgta
     9181 acagattcaa ctgcaccttg agatatttgg tttacagttt catttactga attccataca
     9241 cctgttctat catctaagca ctcgcctttt tctattggtt gacaaggacc tgttgggtct
     9301 aattctttag tagccttagc atctaaccag ctaactgctc cacaaagacc aagtctttca
     9361 ggagtaacaa cacaaacgtg agctggtgca aatgattgac ataagttaca agaatagaaa
     9421 gtgtcaacac tttcatcaac taatgaagct aatctttcat ctctagcatt gtacttagct
     9481 attgcttcac cttttaattc agaaactttt tcagcatctg taattatagt tatttcacac
     9541 ttatcaacta ctgattcaaa ttcatctaac atcttagcat ataaaacttc tcctatatgc
     9601 tcaagtctaa atcctttatc aaatgcatct ttagagattc ttacccaagc catatctctt
     9661 tgtccaacgt gcattacacc ttctatatag tttaagaagt agtggaaacg tctttcaaga
     9721 actggctcga aatcttcttg catattttta ccagctattt taactattac agcaagtggt
     9781 aatcttaata caccatctgc atcaacttca tctatatttg gtcctattat ttctatctta
     9841 tggtcttcaa cttctgttgc ttctttctta acaacaagct cccaagcttc agttctgttt
     9901 ccaccaaact cagcaaacat atcgctcttt ctaattctct caccttcaaa tgctgctgca
     9961 aatgaaactg ggataggtat ttcagtaact tttattttta ttcctctagc ttctaatgaa
    10021 gtagctacta tcttatcgta atctttttga gttaataggt tcattggaac ttcaagaact
    10081 gtttggtcag ttataactgg gaatcctaaa gctattgctc cagcacctgc agatacaact
    10141 aattcactta atggtccaaa tgcattaaca aatgcaggaa ctctattagc tgtgtactct
    10201 aataatccgt ttaaatcacc aggagttaat ccaccgaata ttaatgctgc tcttactgca
    10261 acagatacaa cgtgtataac agaagttaca tcatatccta gaggtataac tcttagctca
    10321 agacccattt ttactccagc ttctatagct tggtctataa ctttaccaac taagaaagtt
    10381 agtaaacctt tagattggta atcttttata acttttgctg ctgattctgc atctggacac
    10441 tcaccaagaa caactgcaac accaggtata tctcctgtaa ctaatggtac accaagtgat
    10501 ctgattatag ggtcagttat atgacctgca catggctcac tatatggagc atccatagtt
    10561 gaatatttta atgcttcaat tacttctgct gctaaagctg tagctaaacc agcattaaaa
    10621 gcatgttcta gtaaatgagt tctatttatt aaagatttta ctacttctaa agcaccttct
    10681 aagtccccta aagtgttcat cttttgtcct gttgcagcat atatacaagg taatgaatat
    10741 gctgtatcag ggaaagcaac tttatgctct tttccattct tttctatagc ttcagctaac
    10801 atagcttgag ccgcacctaa agcttgttct gaccctgtaa agattatatt atatagattc
    10861 attctatttc cccctttaat atttacacct attttttatt aactataccc taaaaaccct
    10921 agaaagcaaa tccaccttca tttaactcta agaaagagtc tgcatataaa gatgccccgt
    10981 tgattatgtc acaagatttt acagttttca ttaattctgg gtcacatgga ttcattatag
    11041 ctgaactaaa tccattagcc attgccattg ctaagaatgc actatctaaa acaggtctaa
    11101 catgcttagg acatccatta gatacgttag ataatcctcc tgtagttaaa agtcccatct
    11161 cagtcatcat ttttatagct tctaaaactt ctacttgttt ttcttgcata ccttttataa
    11221 ctaagcataa tgggtcaaat agtatatcag ttggctccat tcctaacatt aagccttttt
    11281 ctaatatttc ttggcagtaa gccatacgct catcattatc tcttgggata ccttcttttg
    11341 cacataaacc tataaccatt gcttcgtatt cagctgctat atctattaaa tcaaatcttg
    11401 agccagcatc agcagagttt attattggtt ttgcattagt tctatcataa actttaagtc
    11461 cagcttcgat agctttttta ttagctgtat ctaatgctat aggaacattg ttgaactcag
    11521 attgaagtaa tttgattccc catgtcatta tttcttcgcc atctctttcg gcaggtccta
    11581 tattaaaatc tatatagtgt gctcctgctt ctaattgctc ttttgctctt tttaagattg
    11641 gagctgggtc tctttctgct aatgcttttc ttattgaagg tgatatacaa tgtattctct
    11701 cacctataat cataaatttt tccatttata atgtccccct ttaaatttaa tattaatagt
    11761 tagttaatat tttattagtt tgtttggcat gctttttctt ggtatccttt taagaactta
    11821 ggtaattcca tagattcctc tggtcctata actacattcc atcctggtaa gttatcttct
    11881 atatcacctt taagtactgc aacttttcct ggaatgataa ggtctttaca attagttact
    11941 tcttctacct tactttcttt aatgaaagca gagattgagt ttccaccaaa tttaccagca
    12001 gcccaagatg taagaactga atatccacct gcatctggta ttactaacca tactggaact
    12061 tttgatcttt ctatatcacc agcaactatg aagtaagtta atgcaaagtc aacagtaact
    12121 aatactggag aattttcatc tgggttgttg attggataaa tctttggttc aactctcatt
    12181 ggtctttgtg ggtcagtgta tatgttttgt cttagtgcaa ataatggtaa tgccttagca
    12241 taactaatat catctattac tattatagaa ccgtatttta tagtgaatat tgatgataaa
    12301 gcaacttcca tcatagggtt tgaattagat aatctatttg caaatactat tgaaggatat
    12361 ccaaatgttc tatcttgctc ttttaaagct gttcttctaa cttgtatagc attagtgtaa
    12421 gtatctttta tgttttctcc tgttacatct aatactaatt ctttatatcc agcagcttga
    12481 attaattcaa ctgtttcata taattcttct aagctaccag cttttacacc taatggtaaa
    12541 cttgctcctt taactacttc tatcatatct ttgtagttct ctttagttgc tccatatacc
    12601 attggtctag catcttttaa tacttcaaca gcttctttaa ctacttgtgc atcatcacaa
    12661 gctaaaatat aagctacttc taatccactg ccttttattt tatctattaa ttttaaatac
    12721 gcatctttat ctccacaata ctctaaaaca gccatttcaa ctttcatttg ttcaccaatt
    12781 cttacatagt caactttttt catgttagct attttagcat ctactgcttc atcactcata
    12841 caagtacaga atgaaactgc atatctattt ctacttacta atgttttttc atgtctaaat
    12901 aatactgttt ctccacctag ttcatactca gatgcaccag caccaacttt taaagctttc
    12961 attaaaggtg cagtagcctc tgataatttt gctatagcgt catcagacat atgtgggcat
    13021 ttacctactt ctacagctcc tgaagcaacc ttcatagaga aagccataca agttgggaat
    13081 ccacaatcct tacagttttt ctttggtgtt aatttaaata tatctaaagc ttttaatgcc
    13141 attatttttt ccccctaacc ttaacaattt aattaagcta atgcgttaac caattctttt
    13201 atagtttcta ttgattttgg atgacgaagt ataactgcgt ttgaaccacc tactaaaaca
    13261 cttgctgcag ttgaaacttc cattgctata gttctttctt ctggacaacc ccaatctggc
    13321 tcatcttcta ttggagctat agcttcttta acatgaccta cttcaaaagc tactggtgtt
    13381 atgataggca tttgtaatgt cttatcattt tgaccaaatg ctgcaagtct aattctatcc
    13441 atagtagatg caacgtactc atatccataa ccaactgctg aacatccaac attcataact
    13501 atattttcac ctttaacgcc tagttgagtt aacaatacgt ttaattgttt agctaggttt
    13561 atatcaacag aagattcagc tcctacttta tgtgcataag ccataccagc tgatgctcct
    13621 actcctttgt agttatcttc tactgctgac ataaataagc agttatgtcc atccaatgtt
    13681 tgagctaatt tttcaaataa tttaccatct ttttcatgat ttcctgaacc agctacaact
    13741 aaaggtaatt ttattgcttc aattactgcc ttagctacat cagcacattc atcaactgac
    13801 ttatctaatc catttgggtc agaaccatca aattttaaac aaataaaatc tgcttgtgta
    13861 ttagcttcaa catatttagc ccactctact ggacaattag ctacatcttt gtataattcc
    13921 ttatatgaat cagtccagct ttcaggataa acgtctgata tttgtatccc tatttttgga
    13981 gaattaccta cttctccatc aaaactataa aaaggtaata cattttcccc acctaattta
    14041 attgcttttt cccctattcc tacttcaact tctgatattt ttccagaata tttttgagta
    14101 gacattttaa atgccatatt tttttccccc ttaagcctat tttaattcta atttagcaaa
    14161 aatttctttc atagccactt tagattttga atcttcaggt aaattaacta aaggaatacc
    14221 tgtagaatca tattcatata tcaattcatc cataggtaca acaccgatta gctctaggtt
    14281 atgcttttca atttcttctt taacaccatc attcataacc ccatttggaa ctttatttac
    14341 aatcaaaagt attcttccaa cacttaactt tagttcctca gctaaatctc ttattctagc
    14401 aacagcttgt atgctacgtc ttgagcaatc actaaccaat aaaagtgtat caacatgtct
    14461 agtgactttt ctacttagat gttccatacc agcttcgttg tccatgacta agtatttgta
    14521 atgaccagat attttgttta cttgttctct aagtatccca tttacaaaac agtaacatcc
    14581 ttcaccttct gacctaccca ttactaataa gtcatatcct tcgccttctt cgattataga
    14641 atttaatcta aattgaagat attgtgcctt tgtcatacca ccagggaatg cattgcctaa
    14701 cttttctctt tgatttactt cttctcttat ttctcctatt gttgcttcaa cttcgatacc
    14761 taatacttca tttatgttag cattggcatc agcatctaca actaatactg gtcccttttt
    14821 gtccttaacc aaataatcaa tcaaaagccc tgtgagactt gttttaccag ttccaccttt
    14881 tcctgcaaca gctatattgt atcccatttt aagacttccc ccttaaatct agccacagag
    14941 ccttatgtac tctgtctgtc tattataatt taggtacaga atgaacacat tctccatgta
    15001 catcatgaag tgcttccatt agcccttcag ataaacttgg atgtggatgt attgcatccc
    15061 ctacttgttc tactgttaat cctagatgaa cagcaagtga caactctgtc aataaatctg
    15121 tagcatgagg tcctactact gctgcaccta ttatcttatc agtttctttg tcagctataa
    15181 cttttacaaa tccttggaaa tgtcctatag cttgtgcttt tccaagacct ctaaaatcaa
    15241 attgacctac attgtattct acaccttcag cttcaagttg cttttcagtc ttaccaacac
    15301 cagcaacttc aggttcagta taaacacatc ttggaattgc tctataatct actaccttag
    15361 tcttacctaa agcattttca actgctacga taccttcttt agaagcaaca tgtgctagga
    15421 aaggagtatc tattatatcg cctattgcgt atataccttc tacattagtt tcaagatgtt
    15481 cattaactac tacttttcct ctttccattt caattcctat atcttctact ccagaattgt
    15541 caaggttagg tcttctacca acacatacta atgcatattg tgcttctata acctttccat
    15601 tagaaagagt tgcaacagcc ttaccatcaa ctacttcaca agtttgtact ccaatacctg
    15661 taataacctt aattttatct ttcttaaatt gtctaagtag ttgtttagct acatctttgt
    15721 cttcatttaa tagtatttgg tcaaccatct caactatagt tacttctgtt cctaaggctc
    15781 taaagaactg ccctatctca catcctataa ctccaccacc tactattaac atagactcag
    15841 gtatctcttc aagtccaaga acttcatcac tagttattac aacttttcca tcatatggga
    15901 acattcttgg tacaactgga acagaaccat ttgctagtat tatcttatct gcttttatag
    15961 tttctactgt tccatcatct ttagttactt ctatagtgtt tttatcaatt aattttccaa
    16021 aaccattaac taagtttact cctctttttt caaataagaa ctctatacca cttattaatt
    16081 ggtttacaac tttatttttt ctttccatta tggcagtgaa gtttggctta actgtaccat
    16141 ctatttctat accaaaatcc tttgcttctt ttacagtatt caatactcct gaagaagcaa
    16201 gaagtgcttt agttggtata catcctgcat ttaagcaagt tcctccaact cttctttttt
    16261 caacaactgt tacatctgca ccaagcatag aagcttttat agctgctaca tatcctcctg
    16321 gtccaccacc tactactact atcttcatct ttaattcccc caaacctaat ataatctaca
    16381 atcttgcaat actataaacc tgcggcatcc agtatagcag gtatattctc ttctgcccca
    16441 attgccatta tatgaacacc atcacataaa tcttcttctt ttaattgttt gataagttcc
    16501 cctgccattt ttattccttc acttacccag ttttcttttc cagctgctct taatctttcg
    16561 atttgttcat ctggaacaaa tattcctggt acatttgcag tcataaattt agccattcct
    16621 ggtgatttta aaggtactat tcctgctaat accttgcaat ccatatccct tgttaattct
    16681 ctaaactttc tcattgtatt tatgtcataa agtgcttgtg tttggaaaaa tttagcccct
    16741 gcttttattt tctttttcat ttttaataat tgaacttcta ttggagaata ttctggagtt
    16801 actgatgctc ctagataaaa atctggagaa ccttttaact tattaccagc catgtccgta
    16861 cctgccatta aagtctcagc agtttgaagt atccccacag aatctaaatc aaacactccc
    16921 ttagcttgtg gatggtctcc tacgctagta tggtctcctg ttagtgccaa cagattgttt
    16981 atcccaaata cacctgctga caacatttct ccttgtatag ctattctatt tctatctctc
    17041 cctgttattt gaataactgg ctctaatcca gcatctttta ataatttaca tgtagcaaga
    17101 gaagttgctc tcataactgc cgattgaaag tctgttacat ttgctgcatg aactcttcca
    17161 actaacagct ttgcacattc tattaaatgt gaaaggtcag tcccttttgg aggagccatt
    17221 tcagtagtaa ctgcaaattt tccactttct aatgtttctc ttaataagct catctctaat
    17281 ccccctttta accttctaaa aaaaactttt atgaatttgc tgctgcttct ttctttttct
    17341 ttgcacttaa acttcttgga ttattttgct tagaatagtc ttttggtggt cttatttctg
    17401 ctaagttatc taattgtcct atagcttcta gtctttcata tatctttatc caagcacaat
    17461 cattctctga gtttacttca cacttaccat ttttagctcc accacaagcc ccattcataa
    17521 gacctttagc acacatagtt actggacata tcccacctgt ccaaccaagt tcacagtctc
    17581 cacaagcctt acaggcttct tcatattctc caactctttg tacttcccct ataaacaaag
    17641 tattgttggc tggataaact ggcttatctt ttaaattctt aactattgtc tgtgtaccat
    17701 ctccacatgc taaagataaa actgcatcag cttccttagt ctcttctttt agagccttta
    17761 aatccttttt agactttaaa aggttacaag cagggtcaag cattacatat cctaagattt
    17821 ttttgccttc accttcaaga gtttccttca tttttaaaac ttcttcttcc ccaccacttt
    17881 tacaagttgc tgcacattga ttacaaccaa ctaaaaccag cttgtcaaaa tcttttaagt
    17941 accctagcac ttcttctaat ggtttatttt cagaaattat catatctact tcccccctaa
    18001 accttccaca tatatatatc tatttattgt tttgtaattt acaagcctta actacatgtt
    18061 ttgcaagtat agatgttgta actgaaccaa cccccgctgg aactggagtt atcattgata
    18121 ctttgtctaa aactgcattt gtatcaacat ctccacataa atttccttct tcatcaacat
    18181 ttataccaac gtctataact accgctccat ctttaacaaa actttcatca accatcttag
    18241 ctctaccaat agctgctatt agcacgtctg cctctgctgc tacacctgat agatttttag
    18301 tttttgaatg acatattgta acagttgcat gttcacttaa taaaagcatt gatactggct
    18361 ttccaactac cattgatctt ccaagaactg ttaccttact tccttttaaa tcaacattat
    18421 agtgttttaa tatctcaact actgctgtag gtgtacatgg tggaaatcca cttttatccc
    18481 cttccattac cttagcagag tttattggac taaaacaatc tacgtctttt tccggtgcaa
    18541 taacatattt tataacttct tcatttaagt gttttggaag aggtctaaaa cataatatac
    18601 cattaacatt tttgtcatca ttaactctct ttaatacatc aatatattcc tcttgtgtta
    18661 tatcctcagc taactctaac acttctgttg ttatacctat gttttgacat ctctttagtg
    18721 ctcctctttc ataagataaa tcatcacttc ttgccccaac tctaactatt gttagtttag
    18781 gatttatacc ttctttaact agtaaatcga cttcttttat taattcttca ctaatcttat
    18841 cagctacagg ttttcctttt ataatttgtc ctttagttga cattccttcc atacattcac
    18901 aactccccta caagcaaact taaattaatt tattctatat accacatatt aataacatag
    18961 tatctacttt cgtcatttaa aaattatttt ccaagagctt tttcaacttt ggcataaact
    19021 tcatcacaaa ttttcacacc atcttcaact aacttatcta cttctgtttt taccttatta
    19081 acatattctt tatcttgtat agaatttata tttataacta catttaattg tccaccaagt
    19141 atagcagctc ttaaacactg aactcctaca ccaacatcac ttatagcaag tcttgaacct
    19201 ttatcaacta aatcttcatg taacttaata gcttcatagc aaactcttac tatgtttaat
    19261 ggtacttcac aagcaacttt taatgccttt tccattgttt ctgcttttat tcttttttct
    19321 tcttctgttt ctttaggaag cccgtaagct tttgataatg gtaagaaaca ttctgcatca
    19381 tcatctatca tttttaataa atctttttct aactttcctg ccttacttaa tatctctttt
    19441 acactttctt catactcagc atacttcttc ttccctattg taaggttaca taccatacta
    19501 cctaaagcca tacctattgc acctactagt gcagcagctc ccccaccacc tggtactgct
    19561 tctttggaag atagtacttc tacaaaatca acacaagttt tatctgctat tttcatgata
    19621 atcctccttg aaaggatgta ggaaaatatc tcaaattgtt cgagatattt tacgaactat
    19681 tttaagatat ctcctactct ttatttataa attaatttcc ttagaataat ccagctatta
    19741 ctccattttc atcaacatct attttttctg ctgctggaac ttttggtaat cctggcatct
    19801 tcattatttc tccagtaagt gcaactataa atccagctcc tgctgaaatt gttgcttgtc
    19861 ttacagttat tctaaatcct gttggtctac ctaatttagt ttggtcatca gttaaagaat
    19921 attgagtctt agccatacat actggaactt tagtaaatcc aagtttttct aagttagcta
    19981 tttccttatc agcttcagga gtataatcta caccatctgc tccataaatc ttagtagcta
    20041 tagcatttat cttgtctttt atagataaat catcttcata gcagaatcta aagttatttt
    20101 cttcttcatc caataatctt aaaacttctt tagctactgc aattccacct tctccacctt
    20161 ttgcccatac ttcagaaaga gcaacattaa cacctaattc cttacacttg tcttcaacaa
    20221 gtttaagttc agcttcagta tcaagtggga atctgttgat tgcaacaact gctggtaatc
    20281 catatacttg agttatgttt tcaacatgtt ttaataagtt tggaagtcct ttttcaagag
    20341 cttctaggtt ttcgttattt aagtctgctt tagcaactcc accattgtat tttaatgctc
    20401 ttacagtagc aactattata acagcatctg gttttaagtt tgccattcta cattttatat
    20461 ctaagaattt ttctgctcca aggtcagcac cgaaaccagc ctcagttatt acataatctg
    20521 caaagtgcat agccattctt gtagctataa cagagttaca tccatgagct atatttgcga
    20581 atggtccacc gtgtacaaat gatggtgttc cttcaagagt ttgaactagg tttggtttta
    20641 aagcatcttt taataatgca gccatagcac cattagcttt taattgtcta gctgttacag
    20701 gttcaccttc gtagctgtat ccaactatta tgtttcctaa tctttctttt aaatcagata
    20761 tatcacttgc caaacagaat gctgccatta tttcagaagc aacagttata tcgaatccat
    20821 cttgtctaac tgctccatca ccttttttac ccataccatc aacgatattt ctaagttgtc
    20881 tatcgttcat gtctacacat cttctccaag ttattttctt agggtctatt ctaagtgcat
    20941 ttccttggtg aatatggtta tcaagcatag cagctaaaag attgttagca gctcctatag
    21001 cgtgaaaatc tcctgtgaag tgtaagttta tatcttccat aggaacaact tgtgcatatc
    21061 ctccaccagc tgctccacct ttaacaccaa atactggtcc tagagatggt tctcttaaag
    21121 caactaatac atttttgtct agtttagcaa atgcatcagc aacacctata gtagtagtag
    21181 tttttccttc acctgctgga gttgggttta tagctgtagt tagtattaac ttagcttttt
    21241 taccagtctc tctttttagt agattgtagt caacttttgc tttgtattta ccataaagtt
    21301 ctacatcatc ttcacctagt cctaattttt ttgcaacctc tctaatatct tgaggcttag
    21361 cttcttgagc tatttcaatg tcagatttaa atcccataac tgaatctcct cccacaacct
    21421 aaatagattt aaaatataat ttttgtaaaa attgtattaa tatgtaaaaa ttatatttta
    21481 taagataatt atacatgtaa aattacttaa aaactagcta ctaagctatt aaaatattca
    21541 aacttaatag ctagttattt atctaattac ttacttatta cctatctatt gcctatgtct
    21601 aacctttaac caacttaaaa tcccaaatca aaatttctat ctattttatt tatccttaag
    21661 tgacattaat ttatctttta ttgctttaat ctgttctttc gttgtttcac tagaatcata
    21721 tggagattga ttagctctat cagaatcaat aacatcttgg ttataataaa taaatcctaa
    21781 agattctcca tcttctaagt tttggattat aaattcttca tcttccttat ttctaatttt
    21841 atttcctact acaaatactt tatttacacc tatatcatgt ccaagtttct taacttttct
    21901 ataagtctgt agacttctct ctcctggttc tacaactact ataaaagcat ctacaccttg
    21961 ggctgttcct cttcctaagt gttcaattcc cgcttccata tccataacca ctacatcttt
    22021 attttgtagt attaaatgtg agcagagtct cttcaaaaga acatgctctg gacatacaca
    22081 tccagtacca ccagaatcta cggttcccaa tgtaagtaat cttactccat tgtattcttt
    22141 acagaaattt tcagggatat cgtctacttt aggattcatc ttaaacattt ttccaaatga
    22201 acctacagaa gcagctgttc tatctgaaac taattttttc atttctgata taggtactat
    22261 tgattcatat acttcttttg gaaaacctag tgctaaagct aaattagcat cagggtcggc
    22321 atcaacagca acaactctgt atccgtcttc agcaaacatc ctagagagca ttgaagaaaa
    22381 agtcgtttta cctactccac ctttacctgt tattgctatc ttcataatcc ctaccacctt
    22441 ttattactat ttgcttttta gtagttaatt ttacagttaa atatttttta tactctatat
    22501 ttttatacac taaaaataaa ctacagctag tccttacagg aaccaattag atttacccta
    22561 ctagcttttc aaagcagcat gtatagtttt attattcttt aatattgttt atattttatt
    22621 tattcagtat attcattata ttttgatgtt ctttacttta atatttgact ttattcaact
    22681 ttactcaacc agaaacttct tataaaaacg tttattctat aacttcacta ttttttaagc
    22741 ttcagccttt tctgccattt tttcttcaac taatgcttca aagtgaacac gttttttctc
    22801 tatatcttct attattgtag ctgctaactc ttttggattt gtatttacag taaatgtagc
    22861 tccaacttta tcttttaatt cactagttaa tatatccact gctcttgaag aaccagctat
    22921 tggaggcatt attcctaaat aagtgtctat accagaagct acaacatagc atcctatagc
    22981 aactgctttt tctgacatcc actctggagc tatacctaca actggaaggt cacacatatc
    23041 catatctaag taattagctg ctgctccaac caaatctagt atacgactta tatcaacaca
    23101 agaacccata tgtagtactg gtgggattcc tactaattta cagacagttg caagaccttt
    23161 acctgcatat ttttcagccg catcagattc cattaaacca aacttagctg ctgctgaagc
    23221 tccacaacct gtagtaacta ctattatgtc gtttttgatt aattctttca ttattgttac
    23281 gtgagcctca tttgaaactc ctttagcatt gttacatcca actacaccag ctgctcctct
    23341 taatactcca gcttttagac agtctgttaa tggcttaact gttcctgctg ggtctatatg
    23401 agagtttaca actctgtcta attgatttac tactgcttct acactatacc caactgttgc
    23461 ccctgatttt aattcaggta taagtacttt tgatttatct ctatttttaa agtttaaaat
    23521 agcttcttta actattgctt tagcatcatc aagtgcttgt tcttctctaa attcaatata
    23581 agttgaacct gttatcttag cttttggaga agttgttaca aatttagtat ggtaacaatc
    23641 tgcaactcta gctaatgcag ggaatataca ttgaacgtca actattacag cttcaactgc
    23701 tccagttatt attgcaagtt cttgttgatg gaagtctcct gctattttta caccatgtct
    23761 cattgtaatc tcattacctg tacaacacat acctgctaag tttattccat cagcaccaac
    23821 ttctttagct aaagctacta agtctggctc ttctgatgct aaaactatca tttctgataa
    23881 tgctggctca tgtccatgta atattatgtt aactttattt tcttctaata ctgctaaatt
    23941 cgcctctgtt cttcttggca ctggagttcc aaataagata tcgcttagac gagtacctat
    24001 catagaacca ccccaaccat cagccataga agttctaaga ctcatgtgta ttaaactatc
    24061 tatatcacca gtacatccta tatgagtaga gtgcattatt gttgcaattt ctctatctat
    24121 agctcttggc tctatagcgt attctttcca tattttttgt cttggttctg gtgcattttt
    24181 tataaatctt aatgtcccaa aaggttttcc aaattccatt aatgcaattt ctgccacttc
    24241 atgtgctaca tcatagatat ctctaccttc tgtctctaca tcccattctt tagctaaagt
    24301 aattaactta gcttcgtctc ttattttata gtttccattt ggatcagcta atgctaatgt
    24361 atgtgctata tctctagcat ggtctgaatg ggcagctgtc ccaccagcaa ccattcttgc
    24421 aaagtttcta cctactatag tatgctcatc tgctccacat aacccccttg gagtcttagg
    24481 gcttattcta catggtccca ttccacatat tctacagcaa acaccttgaa gaccaaaacc
    24541 acattgtgcc ttaaagcctt cttttctatc atacattgtt tctacgcctt cttctctagc
    24601 tttagctatc atcttctggc tgtttaaatc aattgttaac attttttcat ccatattcct
    24661 atcccctttt tttgattttt aaaatccccc aggtgactga cttcatgtca taaaataata
    24721 aaataaatgc tttctaagtt gattatatac taattttcag tgctttgctt tagatttaaa
    24781 tattttttta acaaaattag ataatacata aaaattttat tttctacact taatttaagt
    24841 tgattttatc gcttgttttt ggctatttgt taatatctaa acctactttt ttgatacttc
    24901 agtttaatta aattatgttt tgattttttt aaatcaaatt tgaaattttt ctagtcattt
    24961 ttatattatt gcagattgtt tgtgtattta ttatctttca ctactgtttg gacttattac
    25021 agatttttac tccctaaact taattagtaa aaatttctta gaatagcatt ttttggacta
    25081 atttttttga atttttttat ttttcctact ttaatattaa ttatagcttc aaattgaaca
    25141 tttgtatata ttcatttgaa gctataatta ttttttatat aaaacttttt taatacagca
    25201 ttaatttaga cattttgact attttttcta ctttaattca tttatttcaa gcgatttatc
    25261 ctatttcata tttttagcta caatagcccc atatgcaata gaatataatt tagattcatg
    25321 tgaatcagct cttgacacta aaactatagg agcttttgca cccataatta tacctgctga
    25381 tttagagtct gccatatatg taaaggcttt ccctattcca ttaccaacct ctattgttgg
    25441 aaccaatatg atatctacat ctccagatac ttcactttta aaccctttta tctcactagc
    25501 ttcttttgaa acagctaaat caaaagccag aggaccttct acaattacat tttcaccaaa
    25561 ttctccttct ttacaagctt ttgctaacat atctgcatct acagttgctt gcatcttagg
    25621 atttactttc tctttagctg ccaaacatgc aacttttaca gtttccattc ctaaagcttt
    25681 tgcagctttt atagaatttt ttaatatctt tgccttttgt tcataatcag gagctatatt
    25741 catacctcca tctgtagtta taagtaattt atggtatttt tctaactcat ataccataac
    25801 atgacttaat aaactatcag ttctaagacc atagtcttta tttaacactt cttttaatat
    25861 gacagatgta tctaataatc ctttcattac aaagtcggct tctttacttg atactaattt
    25921 aactgctatt tctgcacact ctttgatatc atccttatcc actattttga taccacttaa
    25981 atcaaatcct atttcttttg atatttctct tattttgcct tcatttccaa ccaatatagg
    26041 agtaattata tttctttcta cagcatcctt tattgccatt aaaacttctt tatcatgagc
    26101 tgctgctaca gagagtacaa ttttctctcc tcccttaagt tgttctaaaa tatcatctaa
    26161 tttttttatc aaaagtaacc cctcccttat atatatttaa ctaaataata ccatatttgc
    26221 tatacctttt ctcaatttat tttttatgtt ataaagaatt tatataacta aaagacagtt
    26281 taatatttta ttctgggatt tcatcaatag ctcttaaaag ctttgcagga acaccaccta
    26341 caatagtatt ttctgcaaca ttttttgtta caactgctcc agcagcgata attgcattat
    26401 ctccaatagt aactcctgga actacagtta cattagcacc aatccataca ttttttccta
    26461 ttgtgattgg agaaggataa gttgtaccac gtttctctgg tgcaatacca tggttaagtg
    26521 tagccaatac cacattatgc ccaatttgcg tattatctcc aatctcaatc cccccctggt
    26581 cttgaaaatg gcaactgcta tttataaaaa cattttttcc tattgtgatg ttttttccac
    26641 aatctgtata aaatggtgga aacataaaaa atgattcatc tacctttttt ccaattaact
    26701 cagaaaataa actacggact tcttctggtt catgataaga agaatttaac ttacttgtga
    26761 ttttcattgc ttcattactc aacttaacca taaattcatg tatttctgat ttactagaaa
    26821 caggttttct ttgattgata tgtgttaaaa attcattgat attcatttta tacatccttt
    26881 caatatgtgc tttaatttat ttgt
//
LOCUS       DACXMZ010000027        26246 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.027, whole genome shotgun sequence.
ACCESSION   DACXMZ010000027 DACXMZ010000000
VERSION     DACXMZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.027"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            49..963
                     /gene="murB"
                     /locus_tag="JD016_04560"
     CDS             49..963
                     /gene="murB"
                     /locus_tag="JD016_04560"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAU4998901.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            967..1680
                     /locus_tag="JD016_04565"
     CDS             967..1680
                     /locus_tag="JD016_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAU4998902.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            1711..2568
                     /gene="rapZ"
                     /locus_tag="JD016_04570"
     CDS             1711..2568
                     /gene="rapZ"
                     /locus_tag="JD016_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAU4998903.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            2568..3686
                     /locus_tag="JD016_04575"
     CDS             2568..3686
                     /locus_tag="JD016_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAU4998904.1"
                     /translation="MEANMAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVE
                     NREPNVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIR
                     NCLLALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFA
                     ITGRVLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDV
                     IASIYDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYN
                     VLEHVNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGI
                     KTVEKNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            3755..4180
                     /locus_tag="JD016_04580"
     CDS             3755..4180
                     /locus_tag="JD016_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAU4998905.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            4188..5144
                     /gene="whiA"
                     /locus_tag="JD016_04585"
     CDS             4188..5144
                     /gene="whiA"
                     /locus_tag="JD016_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAU4998906.1"
                     /translation="MIDMSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGY
                     KKLNLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEM
                     LLKKLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVT
                     NNEEFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNT
                     KIVKEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIR
                     IEYEDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            5301..8870
                     /locus_tag="JD016_04590"
     CDS             5301..8870
                     /locus_tag="JD016_04590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAU4998907.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNSIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            9079..10038
                     /locus_tag="JD016_04595"
     CDS             9079..10038
                     /locus_tag="JD016_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HAU4998908.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            10090..11850
                     /gene="pyk"
                     /locus_tag="JD016_04600"
     CDS             10090..11850
                     /gene="pyk"
                     /locus_tag="JD016_04600"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAU4998909.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            12025..13389
                     /gene="rlmD"
                     /locus_tag="JD016_04605"
     CDS             12025..13389
                     /gene="rlmD"
                     /locus_tag="JD016_04605"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAU4998910.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETCVLLQ
                     RRTM"
     gene            13634..16687
                     /locus_tag="JD016_04610"
     CDS             13634..16687
                     /locus_tag="JD016_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HAU4998911.1"
                     /translation="MKKMLKRLCTGFLALATIVTALPTTPVHAESKQYWTESAERVGI
                     IEKVMNDGSIGSTFNEGMMKVEGETAYCIDINTDFKNGYKTRTDASSRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSISGATYGVFSDKDCTKQLATLTTDENGNTNVVEVKAGTVYIKELSAPAGYKVDKT
                     VYPLTIKAGETATLKVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKAPNGYLLDGAYMQAGDKSEQIKGLYVTQITEDGDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNAVLVEGKLYKKNEVVKTIH
                     TDIEGVASTSADLLPYGNFRIVESEAPNGYLTDGAKPIDFAITENEKIMDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHIVVTDDNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKNGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELVIDGKRVENDYTFVADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKIAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDITIIDTVT
                     LEGLEVGTQYKLVGWQMLKEKNAELLINGKRVESDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEEEGQTITFKEKPEVPEEPEKPETPQTPE
                     EPSRPSDSPKTGDSTNVIAFLVMLLTSGGGLAGTYLYKRRKMKKS"
     gene            16700..16897
                     /locus_tag="JD016_04615"
     CDS             16700..16897
                     /locus_tag="JD016_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998912.1"
                     /translation="MKKGKSRSPPKLSNGRLPLILACLPTTCKNTDSQGCVSIYFTLY
                     YRWDCWYFPNRQNKKSQSINL"
     gene            16926..17252
                     /locus_tag="JD016_04620"
     CDS             16926..17252
                     /locus_tag="JD016_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 domain-containing protein"
                     /protein_id="HAU4998913.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            17268..17651
                     /locus_tag="JD016_04625"
     CDS             17268..17651
                     /locus_tag="JD016_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 domain-containing protein"
                     /protein_id="HAU4998914.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNAGNPQNHTPQGQTKK"
     gene            17742..18356
                     /locus_tag="JD016_04630"
     CDS             17742..18356
                     /locus_tag="JD016_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998915.1"
                     /translation="MSEKLLDLLKIFLEKYLVPTVIAIVFSFIAYYVTPTDNKLLVKF
                     TIWGYAVFLFCVWFLIIELVIWLVQKIQYHNYSKGVEQRNKQRQAEEWQQSLEFLWTD
                     VDNLSSSDYKLLLRFIKNENQPYCSSGTPMGHCLLNSDWVHKTVSKPAKRVLIKSNQR
                     TSSKAIPIPVYETISETYQYILREDIYQMLKYSYEKYGKISHFK"
     gene            18423..19817
                     /locus_tag="JD016_04635"
     CDS             18423..19817
                     /locus_tag="JD016_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAU4998916.1"
                     /translation="MRKIWNKGHRIRASDKHLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLEKGLTLSPYNLITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNSFYEGMVQRSEEMKQHSNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLKTIGSLAQARQ
                     DGTATCEAKGDGTD"
     gene            20001..21197
                     /locus_tag="JD016_04640"
     CDS             20001..21197
                     /locus_tag="JD016_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAU4998917.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LVDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLNEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEKQATLSEKHEMIIKQQTTPA
                     KDLVES"
     gene            21210..21344
                     /locus_tag="JD016_04645"
     CDS             21210..21344
                     /locus_tag="JD016_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAU4998918.1"
                     /translation="MWELVKDFLLVSMGMGIGVVLMCILSIGKEADYEMKQLEESEDN
                     "
     gene            21345..21566
                     /locus_tag="JD016_04650"
     CDS             21345..21566
                     /locus_tag="JD016_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998919.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            21774..22415
                     /locus_tag="JD016_04655"
     CDS             21774..22415
                     /locus_tag="JD016_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HAU4998920.1"
                     /translation="MEYKYTKIDLEKWNRGKLFQSYIDDMRIVMSLTVDIDVTNLIEF
                     SKSHDLKFYPAMIWVVSKVVNEHDEFKYSWDNKGNLIKWDYISPSYAEFHKEDENFTK
                     MVTDFSDSIFEFHERFMLDREKHKMERAFVANQPLNFFDVSCLPWVKYNHFDVHVFDE
                     GKFLAPVITWGKYELERERYIMPLTMNIHHAVADGFHLSRFFNEVQELINSIS"
     gene            22498..22794
                     /locus_tag="JD016_04660"
     CDS             22498..22794
                     /locus_tag="JD016_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU4998921.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWHEMKRLNPADYEEKQL
                     EDFSKYVFGMSYQTIKDVRKQQKGREEQCRKQGCWWKREEQLAKKQYHTGLTCR"
     gene            22713..23216
                     /locus_tag="JD016_04665"
     CDS             22713..23216
                     /locus_tag="JD016_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAU4998922.1"
                     /translation="MQETRVLVETRGTTGEETISYWFDLPIDVAEFEEKLGVGAESGD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSSDMQEEYVSLLTVYENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            23234..23737
                     /locus_tag="JD016_04670"
     CDS             23234..23737
                     /locus_tag="JD016_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAU4998923.1"
                     /translation="MIDDMAVYIANLGRYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYFYPDCETMTDVAYYYIDELNALGDIPANLQNYIDYEAYGRDLDMGGRFIETSR
                     GICEIPY"
     gene            23855..24253
                     /locus_tag="JD016_04675"
     CDS             23855..24253
                     /locus_tag="JD016_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU4998924.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            24231..>26246
                     /locus_tag="JD016_04680"
     CDS             24231..>26246
                     /locus_tag="JD016_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAU4998925.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIHDFGYLMEHLYGRDGIAYEDYEYQLPKKKLKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNVIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSENRGLLHVIEELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFA"
BASE COUNT         9547 a         3745 c         5631 g         7323 t
ORIGIN      
        1 taaataataa tgacagctaa agttcagtat aatttgggag gaaatacaat gaataatcaa
       61 gatatatata taaatttatt aaatatatta agtaaagagg atataaaagt cgatgaacct
      121 atgaaaaaac atatttcatt tagggtagga ggaccagcag atatattagt tagacctaga
      181 actgaagaac aattaaaaaa tgtacttaag ttggtaaaaa aagaatctat tccgtattta
      241 ataattggaa atggttcaaa cattttaatt aaagatggtg gaataagagg agtggtaata
      301 gaattagctg ataatttcaa ttcttatgag ataaatgata ctaggatgac tgctcagtca
      361 ggagctttat tatcagtttt agggaaagct ctccaaaagc aagaattaaa agggtttgag
      421 tttgcatcag gaatacctgg cacattggga ggagcattag ccatgaatgc tggagcatat
      481 ggtggtgaaa tgaaggacat agtgaagtca gtaagactga tggatatgga aggcaatata
      541 tttgaattat caaatgaaca gatggaattt gggtatagaa aaagtataat atcgaaaaac
      601 ggatatatag ctttatcagc agaattagaa ttacaagaag gaaattatga tgaaataaaa
      661 agtttaatgg atgatttagc gacgagaaga ataactaaac agccattaaa ttttgctagt
      721 gcaggaagta catttaagag accaacagga tattttgcag gtaaattaat agaagaaact
      781 ggattgagag gtcttacttt aagaggagca caagtatctg agaagcattg tggatttgta
      841 gtcaatcaag gagaagcaag tgctaaagat attttggatt taatatatgt tataaaaagt
      901 gctgtatatg ccaagtttgg ggttatgttg gaagaagaag tcaaaatact tggagaggat
      961 taacaaatga aaatactggc tgattatcat acacatacat tatatagtca tggcaaaggg
     1021 actattgagg ataatgtaaa ggtagccatt tctaaaggaa taaaaacaat aggaatatct
     1081 gaccatagtt ataaacatat tgcatatggt gtaaagaaaa aagatattct caaaatgaga
     1141 gaagaagtag attatttaaa tgataagtac actgatataa atatactttt aggtatggag
     1201 tgtaacatat tagatgataa aggaaatata gatatggatg ataaatttat tagagatttt
     1261 gattatatta tggctggata tcattttggt tctactccaa catcattaag aagcttatta
     1321 aatcattgta ataactatat ttttaaaact gaaaaagcaa aagaatataa tacaaaagct
     1381 gtaataaatg cgataagtaa taatgatatt tttataatta ctcatccagg agataaaggc
     1441 gatgtgtata tagaagaagt agcaaagaca gcaaaaaaga caaatactag acttgagata
     1501 aatagtagcc attctttttt gaatgtaaat caattaaata aaataaaagg gctagaaaat
     1561 aaatttataa taggctcaga tgcacattgt ccagaaagag ttggtgattt tgaactagct
     1621 atgaagacta tatctgactc taatctggat atatcgttag tagaaaacat tataatataa
     1681 taaacataag ataaaaggga ggagttagca atgaaatttg ttatagttac aggtctttca
     1741 ggatctggaa aaagtgagac aatgagagct ttagaggata tgggatttta ttgtgtagat
     1801 aatctacctc ctgctttgat aactaaattt gcagaattat gttatcaacc aaattcaagt
     1861 attgataaag ttgctctagg gatagatata agagggagaa agttttttga agcgcttcat
     1921 gaaagcttaa attatttaga aaaagaaaat tatgaatatg aaatggtata tttggattgt
     1981 aatgacgatg tattgttaaa gagatataaa atgacaagaa gaaatcatcc attggcaaaa
     2041 gatatgcaga ttccagaagg tataaaaatg gaaagaaaga taatggaacc tttaaaggag
     2101 ttatctacat gtataattga tacaactaac atgaaaccta aagaccttaa ggaagaaata
     2161 aagaaaatat attcttctgg tgaagataat ccaaacctaa ctatttctgt ggtatctttt
     2221 ggatttaaac atggtatact tgctgatgca gatttagtat ttgatgtaag atttcttcca
     2281 aatccctatt atgttgaaga gttaagagct aaaactggtg atgataaaga agttagagat
     2341 tatgttatga attctaaaat cagtgaagaa ttttatgtga aacttttaga tatgattcat
     2401 tttttagtac ctcaatatat agaggaagga aaacaacatc tagtaattgg tgttggatgc
     2461 acaggtggaa gacataggtc agttactata acaaatctta tagcggagga tttatctaat
     2521 aagggatata gagttgtaaa gaaacataga gactctatgt tgcgataatg gaggcaaaca
     2581 tggctaaatt acttattatt gtaggaattt taggttggat tgcattaatt gtatcgttat
     2641 ttatatataa aaaagcaaaa aatgaaaaag taaaactatt tcaaaggtct attgttgaaa
     2701 atagagaacc aaatgtggtt gtgataggtg gaggtactgg tcaatcggta tttttaagag
     2761 gtcttaaaca cactacgcaa aatataactg caattgtaac tgttgctgat gatggtggag
     2821 gttcaggggt attgagagaa gacttaggaa tgctcccacc aggagacata agaaactgtc
     2881 tattagcact tgcaaatata gagccaacaa tgaatgaagt tatgcaatat aggtttacag
     2941 aagggctttt aaaaggtcag agttttggaa atttgttttt ggctgctatg aatgggttgt
     3001 atggaaactt tgaaaaagct gtatataaat taagtgaaat ttttgctata acaggaagag
     3061 ttttaccagt aactttggaa gatgtaaatt tagttgccaa attagaaaat ggcaatataa
     3121 taaatggaga atccagtata cctgaagagt caaaaattca aaaaagctct attgataaaa
     3181 tttttttaaa tccaaaagat gtaaaaccat taaaggatgt tattgcatct atttatgatg
     3241 ctgacattat aattatgggt cctggaagtt tatatacaag tataataccc aatttacttg
     3301 tagaggggat tgtagacgcc attaaatcgt ctgtagctcc aaaagtatat atagcaaata
     3361 taatgactca accaggtgag acagaagggt acaatgtatt agagcatgtt aatgctatag
     3421 taaaacacac tgatgagaat ttaatagatt atgtaatcgc aaataatgag atattgccag
     3481 aagaaatgct tgacttatat aaacaagatg gagcagagca agtcttgtta gataaaaagc
     3541 aaaaagataa attaaaagag atgggtataa aaactgtaga gaagaattta attgaaataa
     3601 aaaataatta tataagacat gatgcaaagt atatatctaa catagttatt gagttagccc
     3661 taaatcataa ttacaacaaa agttagtaaa aaaggatttt aaaaaattgt gtagaaatcc
     3721 ttatcattat gttagtttta aggagtgaac atcgatgaga gaagaggtta gtgctggtgg
     3781 tgtagtcctg tttggcaata cgattctttt actaaggaag ttcaatggag attgggtgtt
     3841 acccaaggga aaagtagaag aaggggaaaa taaccaagag gcagcattac gtgaagtaag
     3901 tgaagaaact ggagtaaaag cagatatatt gaaatattta ggtgaaatac actatacatt
     3961 caaagaaaac tgggatgaaa atagagcagt tcataaaaca gttttttggt atttgatgca
     4021 agcaaagaat atggatacta ttccacaaaa agaggaaggc tttattgatg ctaaatttat
     4081 ccacctagat agagtagtag acttagctag gtatgatgat gaaaaggaga taataaaggt
     4141 tgccttacaa gaaataaaaa aaaggttaaa gaaaaattag ttaataggtg atagatatgt
     4201 ctttttcaac tgaaaccaaa aatgaactag caagagttat atctgggaat gaatgttgta
     4261 atatagctga attgtcagct ctagttaaat cagggggaag tatacaaatt gttggatata
     4321 agaagctaaa tctaaagata acaacagagt taaattcaat agcccgtaaa gtatttaaat
     4381 tattaaaaaa gaattttaat attaatacta caatctcagt aaataagaat caaatgctaa
     4441 agagaaataa cagttatgta ttaatggtaa caagcgaaat gggttcagaa atgttattaa
     4501 aaaagttagg tatacttgaa gataaagaag gattttttac tataaacaaa atttcagaga
     4561 acttaattaa gcatgaagga tgtaagagag ctttcataag aggagctttt cttggtggag
     4621 gttctataag cgacccagaa aagaactatc atatggaatt tgtaactaat aatgaggagt
     4681 ttgcagattc tctaaaagaa cttataaact ctttaggttt taatagtaaa attgtggcta
     4741 gaaaaaataa tcacgttgtt tatatcaaag agagtgaaca aatatcagat ttgttgagta
     4801 ttataggagg acaccacgct cttttaagtc ttcaaaatac taaaatagta aaagaaatga
     4861 gaaataatgt aaacaggata gtaaattgtg agacagctaa cctttctaaa acagtaaatg
     4921 cagcagtaag acaagtagaa aatattaagc tcatacaaga gactatagga attagcagtt
     4981 tgccagaaaa tttacaagag atagctaaga ttagaatcga gtatgaggat atgactctca
     5041 aagaacttgg agagatgtta gaaccaccaa taggaaaatc tggagtcaat catagactta
     5101 gaaaaataga agaaattgca acagatttga aaaaaaaatc atagcaataa attgagggga
     5161 caatctcaga ataagcatta atttataaca tataatggta tatgttgaag ttaattccta
     5221 ttttgagata aaagtctctg tttttgtttt agtacatata tttgtggtaa aattaaaatt
     5281 ataaagcagg aggtgattta atgaacaaag attttgctca tcttcatgta catactgagt
     5341 atagtttact tgatgggttt tcgagggtga aaaaacttat aaaaagggct aaggaactaa
     5401 acatgagctc aatagctata actgaccatg gttgtatgtt tggtgttata gatttctaca
     5461 aaacagctat aaaagaagga ataaagccaa ttatagggtg tgaagtctat acagctgcac
     5521 gtggattgag agataaagac ccaaactatg acaaatatca aggacattta gttctgttag
     5581 ctaagaatat ggaaggatat aaaaatttaa taaaaatagt ttcaacttca tacgtagaag
     5641 gtttttacta taagcctaga gttgacatgg aagaacttaa aaaacataac aaagggatta
     5701 tagcactttc tgcatgcctt gcaggagatg tagcacgtgc tttaatgaat agaaattatg
     5761 aaaaagcaaa acaatttgct cttgactaca gagacatatt tggagaagaa aatttctttt
     5821 tggagattca agatcacaat ttacctgagc aaaaagaagt aaactctggt ttagtaaaat
     5881 tatcaaaaga gactggtata ccactagttg ctactaatga tgttcactat gtaaataaag
     5941 aagattcgaa aatacatgat gtacttatgt gtattcaaat gggaaaaaca ttaaatgacc
     6001 caaatagaat gagatttggt agtgatgagt tttatttaaa atcaagagaa gaaatggaag
     6061 aattatttcc atatgcatta gaggctattg acaatactgt gaaaatagct gatatgtgta
     6121 atgtggagtt tgattttaat actatacatc ttcctaagta tgatgttcca gaaggatata
     6181 caccagaaac ttatttaagg gagttgtgtt ttaatggatt aaaagagaga tatgataatc
     6241 ctagcaatga gatactagag agattaaatt atgaattaga tgtaattgaa aaaatgggtt
     6301 atgtagagta ttttttaatt gtttgggatt ttataaactt ttctaaagaa aataatatta
     6361 tagtaggacc aggtagaggt agtgcggctg gttcgatagt agcatataca cttaaaataa
     6421 ctgatataga cccaataaag tactctctat tatttgagcg ttttttaaat ccagaacgta
     6481 tatccatgcc agatatagat atagattttt gttatgagag aagagaagaa gttatagatt
     6541 atgtaaagag aaaatatggt gatgaccatg tagcccagat aataactttt ggaacaatgg
     6601 gtgcaaaagc tgcaataaga gatgttggaa gggtattgga tataggctac aataaagttg
     6661 acaaggtagc caaggaaata ccatttgctt taggaatgac aatagataag gccctagata
     6721 caaatcctaa tcttaaagaa ttgtatgacc aagacccgga aacaaaagaa ataataaata
     6781 tatcaaaaca gatagaggga atgctaagac atgcttctac tcatgcagca ggagttgtta
     6841 tatcaaaaaa tcccgtagat gaatatgtac ctttgtacaa acatcaagat gcaatcacaa
     6901 cacagtttac tatgacaaca cttgaagaac ttgggctttt aaagatggat tttcttgggc
     6961 taagaactct tacagttata agagatactc ttgatttaat agaaaaaaat agagttataa
     7021 aaaattacac agaaaaaata gacttttcaa agatggatta tgacgacccg aaagtatatg
     7081 aaatgctttc atcaggaaat acactagggg tgtttcaatt ggagagtgct ggcatgagaa
     7141 gttttatgaa acagctaaaa ccagacaatt ttgaagatat tgtagcggga atatccctat
     7201 ttagaccagg tccaatggat tctatacctg catatataaa aaataaaagc aacccaaaag
     7261 atgtaacata tttacatgaa aaattaaagc ctataatgga agttacatat ggttgtttgg
     7321 tatatcaaga gcaggttatg caggtcgtta gagatttagg tggttatagt tacggacgaa
     7381 gtgacctagt tagaagagcc atgagtaaga aaaagatgga cgtgatggaa gaagaaagac
     7441 aatattttat tcatggtaaa tttgatgaag atggtaatat agaaattgca gggtgtatca
     7501 gaaatggtgt agaagaggaa atagctaata aaatatttga tgatatgata gacttcgcta
     7561 gatatgcatt taataaatca catgcagctg catatggggt tttagcatat gagacagctt
     7621 atctaaaaac atattatcca gttgaattta tggcagcatt gataaccagt gtaatgggaa
     7681 atactgataa ggtagttgag tatataagag aatgtaaagc actagaaata gatgtattac
     7741 ctccagatat aaacaaaagt ttttcaaagt tttcagttga aggcgacaat ataagatttg
     7801 gtctagcagc tgtaaaaaat gttggtgtaa atataataaa caatataatc gctgaaagag
     7861 aatctaatgg attatttgta gatttagtag atttagtcaa aagattagac caaaaagata
     7921 caaataagag agtaatagaa agtctaataa aatgtggagc ttttgataat ataagtgaaa
     7981 atagagctag tttgatggct ggttatgaaa gtttattaga aagtgtatct atggatagaa
     8041 agaaaaatgt tcaaggtcaa atttctttat ttgatgcttt tggagatagt gaggaagata
     8101 gtgattttca acaaatatac agtttaccta aaaggcaaga gtacgaagaa agagagagac
     8161 ttaaccttga aaaagaagtc ttgggaatgt atgtaagtgg acatccttta tcacaatttg
     8221 aaaaagaact acaagaaaaa acatctatgg ataatggaaa attaaattca ttaaaagagg
     8281 aagaagagtc atacctacaa atgaatgaaa cagatgttat aatgggtgga atgattgtaa
     8341 ctaaaacaat taaaacaaca aaacgaaatg aaattatggc atttattgaa ttagaagatt
     8401 tgtatggtgc tatagaaata atagtttttc ctcaattatt acaaaagtat aatgtcattt
     8461 tgaatgaaga taatataata tatgtaaaag gaacattaag tataaaagaa ggcgaaaatg
     8521 caaaacttat agctagagag ataaaagata taaatgatga tactgacttt gcagatagaa
     8581 actacaattc aggaagaact agaaatgtag aaaatattaa gccagaaagt ggtaaaaaat
     8641 tgtatttgaa aatagatagt atgtctgata ctgatacaac taatagtatt gtagaaatag
     8701 ccaaacaata tccaggtaat gacagtatat acttgtaccc tatggatgaa aatataaatg
     8761 gtaaacgtaa agcatataaa atgaccgggg cattgacttg tatatgtgat gaatttataa
     8821 ataacttaga agagttactt cctaaagata acataaaagt aaaagcttga ttttttatat
     8881 aaaaatctaa atatattata ataatatgaa aaatattgtc cgttttgtga tataatatca
     8941 caaaatggac atttaaggac atttaatttt tttagtcttg atgggattaa aaagtgatat
     9001 agggacaaaa aaagtccatt agaatacaat aacaatgatt ataataaaaa attgacataa
     9061 aatccaagga ggtaaaaaat gaaaactata ggattactaa caagtggagg agatgctcca
     9121 ggaatgaatg ctgcgattag agcggttgta agatcagcaa tatattatgg atgcaaagta
     9181 tatggaatca atagaggata taagggatta ctagaagaag atttaactga aatgaactta
     9241 tcttctgttg gagatattat acacagggga ggaactatat taaaatcttc cagatgtgaa
     9301 gaatttaaaa cagaagaagg tagattaaaa gcagtaaaaa ttcttaaaaa gtataaaatt
     9361 gattgcttag ttgtaattgg tggagatggt tcatttgcag gagctcaaaa acttagtgac
     9421 ttaggattcc cagctatagg cataccagga actatagata acgatttagc atatacagat
     9481 tatactatag gatttgatac tgcaatgaat actattatag atgctatagg taagataaga
     9541 gatacatcat catctcatga aagagttaac atagtagaag ttatgggaag acactgtgga
     9601 gatttagcct tatatgcagg tcttgcagga ggagcggaaa ctataatagt accagaagta
     9661 gaaattacag tagatgaagt tgctctaaga ttaaagacta ctcaaaaaag aggtaagaga
     9721 catagtataa tcgttttagc agaaggagtc ggaagtgcta gtgatttaga aaaggaactt
     9781 aaaaaagagt caggagcaga tttaagagtc actgtattgg ggcatgtcca aagaggagga
     9841 agcccaactg tatctgatag aatactagca agtagacttg gagtaagagc agtagaatta
     9901 ttattagatg gaaaatcagc tagagttgta ggaataaaag aaaacaaaat aattgattta
     9961 gaaataagtg aagctttagc tcaaaagaaa gtatttgata aagaagctta tgagatggca
    10021 aaaatattat ctatataagt tttagtatta aaaataaatc tgtggaataa tatctcagga
    10081 ggagcaaata tgttaaataa tgtaaagaag acaaaaattg tttgtacact aggtccagca
    10141 tcacaaagtg aggaagtttt aactcaactt atgcaaaatg gtctaaatgt gtgtagattt
    10201 aacttttctc acggttcgca tgaagaacat aaagaaagaa ttgatatggc aaagaaagtt
    10261 agagaaaaat taaataagcc agttgctata ttattagata ctaaaggtcc agaaattaga
    10321 acaggtaact ttgaagaccc agaggtattt ttagaagaag ggcaaaagtt cactataact
    10381 atgaaagata ttatggggac taaagaaatg tgtacagtaa gctataaagg tcttgctgaa
    10441 gacgttaaat caggagattc tatactaata gatgatggtt tagttggctt aagagtaaaa
    10501 gaaataaatg gagaagatat agtttgtgtt gttgaaaatt caggaatagt taaaaatcat
    10561 aaaggagtaa atgtacctgg tgttaagata aatttaccag caataactcc taaagatata
    10621 agtgatatag agtttggaat aagtcaagga atagattata tagctgcttc ttttgtaaga
    10681 aaggcatctg atgtactagc aataagagaa gtattagaga acaataatgc aactgatata
    10741 caaataatat ctaaaataga aaaccaagaa ggtgtagaga acctagatga aatattaaaa
    10801 gtttctgatg gtataatggt ggctagaggt gacttaggtg ttgagatacc aactgaagag
    10861 atgccaatag ttcaaaaaat gatgataaaa aaatgtaatg aattagcaaa gccagttgtt
    10921 actgccactc aaatgcttga ttctatgata agaaatccaa gaccaacaag agcagaagtt
    10981 acagatgttg ccaatgctat atatgatgga acagatgcga taatgttatc tggagaaact
    11041 gctgctggaa aatatcctgt agaggcagta aaaatgatgg ctacaatagc aaaaagaaca
    11101 gaagagactt tagattatga tagattacta aaggaaaatg gtactaacaa tgttactgta
    11161 acagatgcaa taagccatgc aacttgtaca acagcagtag atttaaatgc atcagctata
    11221 ataacatcta cttcttcagg atatactgct agaatggtat ctaaattcag accaaaatct
    11281 ccaataatag ctactacaaa taatgaaaag actatgaata aattagctct tacatgggga
    11341 gtatatccag ttaagtcttc tgttgcagga aatacagatg aagttataga aaaggcaatt
    11401 gaggcagcta gacaagctga ttatatagat aatggggaat tagtagttat aactgctgga
    11461 gtaccagtag gagtaagtgg aactactaat ttaataaagg ttcatgtaat aagcgaagaa
    11521 atcgtgcaag gtataggtgt aggtactcaa acagtagaag gtaaagttcg tataataaag
    11581 aatggaaatg cttgtgtaga ctttaatgaa ggcgacatac ttgtttctac tgctactgat
    11641 gctgaaatga acaattatat agaaaaagca ggagctgtag taactgagaa tggtggaatg
    11701 acatcacata cagctattgt aggtataaat ttagatatac cagttattgt gtctgcagct
    11761 gatataacaa atctagtaaa agatggagaa gttgtaactg tggatgcatc aagaggcgtg
    11821 gtatatagag gttcaactag agttttataa gaaaaataat aaatttattt actaaggggc
    11881 tttataagct cctttttttt tggtaaaata aaatattgta gcttagtaaa aaatatagtt
    11941 taattaatag caatatttta atataaattt aaagaaaaaa gtttgtaaag atttaaatat
    12001 aatagattga ttggagggtg atttatgctt tcgaaaaacg agatgtatat tgtagatata
    12061 gtagacattg gtcaaggcgg agtaggagta ggaaagtaca atggtattac agtttttgta
    12121 gagggcggat taatacatga taaagttaaa gttaggatta ataaagtaaa gaaaaattat
    12181 gctgtgggag atattgttga gataatagaa aagtctccat tcagggtgaa aagagtgtgt
    12241 agtgacaatc ttagggattg tggagggtgt cagatacaag acttagatta taataaacaa
    12301 cttgaaatga aaacaaatga agttaaacaa gtactttcga gaataggtaa gttagaggat
    12361 gtagaagttc atgacactat aggaatggat aatccatata gatatagaaa taaagcacaa
    12421 tttccaatac aaaagaataa tggtattcca attattggct tctataaaaa gaagactcat
    12481 gatgtaatat cgacagaaaa atgcgtaata caacatgaga taaatgataa aattgttaaa
    12541 ataataaaaa catatataag ggcctataag gtaagcatat atgatgaaaa gactcacacg
    12601 gggttgatta ggcatttagt aacaaaaata ggttttacaa ctaaagaagt aatggttgta
    12661 ttagtagcaa atggaaacaa acttccatat cttaaagaat tagcttcagt gcttaaggaa
    12721 aatatacctg gatttaagac tttggttgta aatgtaaatc aaaaagatac gaatgctatt
    12781 ctaggaagag aaaatatagt tatatatgga gatggaaaaa taaacgatta tataggagac
    12841 ttagtgtttg agatttcacc attatcattt ttccaagtaa accctgtcca aactaaagta
    12901 ttgtatgata aggctcttga atatgcaaat ttaaatgata ctgatactgt ttttgatatt
    12961 tactgtggga taggtacaat atctttattt ttggcacaaa aggctaaaaa ggtttatgga
    13021 atagaaatta tagatgatgc cataaaagat gctaagataa atgctaaatt aaacaatata
    13081 cataatgctg aattttatgt tggaaaggca gaggaaattg tacctaagat gtatagagag
    13141 ggtaagactg ccaatgtagt cgttgtagac ccacctagaa agggatgtga cgaaaaggta
    13201 ttagagacta ttgtttcaat gaatccggaa agaattgtct atgtttcttg taatccttct
    13261 actttagcaa gggatttaga ttatctaaat gttcacggat ttaaatgtgt tgaggcacaa
    13321 cctgtagata tgttccctca ttctacacat gtggagactt gtgtgttgct acaaagacga
    13381 actatgtaga aatccttaat tttaggcact ttttcaagca tatcgtcttt acgagcgagg
    13441 gcgataaaat cagaaataaa gagataaaaa actatatcaa tgtttcggta gtagttgata
    13501 tagagtgtgg gaacacaaac aattaaatac taaactttag gggtacttgt taaaatagta
    13561 tcccttttta aatgtaagac gttcagaaat gagcgtctat ttttataccc aaaatgaaag
    13621 gagcattaga tttatgaaaa agatgttaaa acgattgtgt acgggcttct tagctcttgc
    13681 aactatcgtt actgctttac cgactacacc cgttcatgca gaaagcaagc aatactggac
    13741 ggaaagtgca gagcgtgtcg gtatcattga aaaagtaatg aatgacggtt ctatcggttc
    13801 tacattcaat gagggcatga tgaaagttga gggcgaaact gcctattgta ttgacatcaa
    13861 tacagatttc aagaatggtt acaagaccag aactgacgca agctcacgca tgagtgccga
    13921 ccagatttca gatgtggcgt tatccttaga gtatgtcaaa cagtatggcg aagctcacaa
    13981 ggaattgaat tacaaacaag tttatctgtt ggaacaatgt gttgtctggc agagattaag
    14041 cgtacatctt ggctggcaat gcgacaacgt gcgagcttct tatgatgaaa taccaaaagc
    14101 tacgcaggac gaagttttcg ctggtgcaaa agcctttgtc aaagaaaata aaggacgcta
    14161 tgaatgtggc ggttatatct actctggcga ggggcaggaa ttgggacaat tctgggcgaa
    14221 gctgaatgtc ggaaatgcga agctgcaaaa gacttccagt aataccagca ttacagacgg
    14281 taacggaaat tactctatct ctggtgcaac ttatggtgtc ttttctgata aggactgcac
    14341 aaaacagctt gccaccctta cgactgatga aaacggaaat acgaatgttg tagaagtcaa
    14401 agcagggaca gtatatatca aagagctttc tgcaccagca ggatataaag tggataaaac
    14461 agtctatcca ttgacaatca aagctggcga aacagcaacc ttaaaagtat ctgatacacc
    14521 aaaggtaacg gatactttga ttgagctttt caagattgac atggaaacac agaaagacaa
    14581 tccgcagggg aacgcttctc tagcaggtgc ggaatttaca tggaagtatt atgctggctt
    14641 ctataacaaa gacaatcttc ctgccgaagc tactcgtaca tgggttacaa agacaatcgc
    14701 tgaaactgac agcgacggga caacccatta catcacaaaa ttagcggacg catacaaggt
    14761 atctggcgac agcttctata tgcaggacgg taaggcggtt cttcctttag gaacattaac
    14821 cgttgaggaa acaaaagctc caaacggcta cttgcttgac ggtgcatata tgcaggctgg
    14881 cgataagtcc gaacagatta agggcttata tgtaacacag attaccgagg acggcgacct
    14941 tgccgtatta tctggaagta accagttttc tgtatcagat aaagttatcc gtggcggtgt
    15001 caaaattcag aaacgagatt tagaaacggg cgataccaaa ccacaaggaa gtgctacctt
    15061 gaaagatact gcttttgaca tcatttcctt aaatgataat gcagtattgg ttgagggtaa
    15121 gctatacaag aaaaatgaag tggtaaaaac aatccatacg gatattgagg gtgtcgcttc
    15181 tacttctgcc gaccttttac cttatggaaa tttccgtatt gttgaaagtg aagctccaaa
    15241 cggatatttg acagacggtg caaaaccgat tgattttgca atcacagaaa acgaaaaaat
    15301 catggactta accgacgaag cccattctat ctacaatcag attaagcgtg gagatattga
    15361 gggcgtaaaa atcggtgcag gcacacacaa acgtcttgct gatgttccct ttaggatcac
    15421 aagcaagaca acgggcgaaa gtcatattgt ggtaactgat gataatgggc aattctccac
    15481 ttctgctgac tgggcttcac ataagcacaa taccaacgca ggcaaaacaa gtgaggacgg
    15541 tgtatggttt ggaacttctg aacccgacga cagcaaaggt gcattgcctt atgatactta
    15601 catcattgaa gaattacgct ctgacagtaa caaaggcttt gagcttatcc caccttttga
    15661 aatcgtggta tcaagaaata accttgtgat tgacttaggg actttgactg atgaatacga
    15721 aaaagaaata tctatccata ccacagcgac cagtaagaac ggcgaaaaga ctatccttgc
    15781 aggaaaagag gtaacaatcg tcgatactgt caaattagac ggacttacaa aaggcacaaa
    15841 ataccagtta aaaggctggc agatgttaaa ggaagaaaat gccgagcttg ttattgacgg
    15901 gaaacgtgta gaaaatgact atacctttgt cgctgatgat gaagaaatga aagtggaaat
    15961 ttcttataca ttcaatgcgt ccgagcttgg cggtaaaaat ctggtaacat tcgaggaatt
    16021 atacgattta agcaatccag acgaacccgt aaaaattgcg gaacacaagg acattgagga
    16081 cgacgggcaa actgtattga ttacagagcg tatcatcaag atacatacga ctgctaccga
    16141 caaggacggc aacaaagagc ttgaagctgg aaaagacatt acaattattg ataccgtaac
    16201 cttagagggt ttagaagtcg gtacacagta taaacttgta ggctggcaga tgttgaaaga
    16261 aaaaaatgca gagcttctta tcaatggaaa acgtgtggaa agtgattata cttttaccgc
    16321 tgacagcgaa actatgaaag tggaagttgc ctttaccttt gacgctactt ctcttgacgg
    16381 caaacagctt gtaacttttg aggaattata cgatttaagc aatccagacg agccgaagaa
    16441 agttaccgag cataaggaca ttgaggaaga gggacagacg attacattta aggaaaaacc
    16501 agaagtccca gaagaaccag agaaaccaga aacgccacag acaccagaag aacctagcag
    16561 acctagcgac agtcccaaaa cgggcgacag tacaaatgtc attgcatttc ttgtgatgtt
    16621 gcttacatct ggtggtgggc ttgctggaac atatctttac aaacgccgta aaatgaaaaa
    16681 atcatagggc ggtaatagca tgaaaaaagg aaaatcacgc tctccgccga agctgtcaaa
    16741 cggcagactt ccgcttattc tggcttgtct gccaacaacc tgcaagaaca cggatagtca
    16801 agggtgcgta agcatttatt ttacccttta ctatcggtgg gattgctggt acttcccaaa
    16861 ccgccagaat aagaaatcac aatcaatcaa cttataaaat atagaaagaa acgaggtaaa
    16921 acaatatgga aatgaaatat gtcgtgccag atatggcaca gtcttttgga actcttgaat
    16981 ttgcaggcga aagcgagcct atctttgaaa gagataaaaa taaccgcagg gttttagcca
    17041 gacgaagcta taacctttat tctgacgtac agaaaggtga aaatgttgtg gtagaaattc
    17101 ccgtgcaggc tggtgaaaag catttcaagt atgagcagaa agtaaaactt gtcaatccga
    17161 agttatacgg cagaggttac gcaatcgggg atatgggaca taccgattat gtattagttg
    17221 ctgatgatat tgtggcagtt gaagaaaagt aaaggagtga agaatttatg agattatcaa
    17281 acggttttgt cattgacaaa gagaaaacat ttggagaatt aaaatttaca gcggtgcgtg
    17341 atgtgttctt gcagaacgag gacgggacac cgagtactca gcttaaaaag cgtatctatg
    17401 atttgaagtg cagtctgcat ggtggaatta tccccgtgtc cgtaccgcca gaagtaccgt
    17461 taagggaatt tccgtacaat gcagttgtgg agcttgtcaa tccagtagcc gatacggtat
    17521 caagaaagac ctatacgggt gcagatgtgg actggtatgt aaaggcagag gatattgtgt
    17581 tgaagaataa aggcaatcaa aacgcaggca atccccagaa ccatacaccg cagggacaaa
    17641 cgaaaaaata attagttcat caaatatatt ttgattgcct ctgcaattaa gaaatcgtat
    17701 aatattttta atgtgatttt ttaattaagg ggcatagatg catgagtgaa aagttattag
    17761 atttattaaa gatatttctt gaaaagtatt tagtaccgac agttattgca atagtttttt
    17821 cttttatagc ttattacgtt acacctacgg ataataagtt attggtaaaa ttcactattt
    17881 gggggtatgc tgttttccta ttctgcgtat ggtttctaat catcgaatta gtaatatggt
    17941 tggtacagaa aatacagtat cataattatt caaaaggtgt ggaacaacgt aacaaacaga
    18001 gacaagcaga agaatggcaa cagagtttag aatttctttg gacagatgtg gataacttat
    18061 cttcaagtga ttataaatta ttgcttagat ttattaagaa tgaaaatcaa ccatattgca
    18121 gttctggtac acctatggga cactgtttat tgaattcgga ttgggttcat aaaacagtat
    18181 ctaagccagc aaaaagagtg ttgataaaat cgaaccaaag gacttcatca aaagcaattc
    18241 ctattccggt gtatgaaaca atctcagaaa cataccagta tattcttaga gaagatattt
    18301 atcaaatgct taaatattca tacgaaaaat acggaaagat ttcacatttc aaataaatcg
    18361 taactagcag ttagtcttag gattgactgc ttttttcgta cccagaaagg agtgattgat
    18421 ttatgcgtaa gatttggaac aaaggacacc gtatcagagc cagcgacaag caccttgtct
    18481 atcacttttc catagggacg cttctgtttg tattcgtggc ggttctcctg ctactgaata
    18541 tcaaacagct catgcgtacc gattgggaac atttcagctt gttggaaaaa ggcttgacgt
    18601 tatcccctta caacttgata accatactga tagcgactgg tgtttgtgca ttggtcgctt
    18661 ttctgtatta tcgcttctgt tacgacagtt tcaagaagct cctgcaccgt caaaagctgg
    18721 caagaatgat actggaaaat aagtggtatg aagccgatac cgtacaagac agcggttttt
    18781 ttactgacct gcaaagcaga tcaagggaaa aaatcgtctg gtttccaaag atttattatc
    18841 aaatggaaaa gggactgctt catatccgct gtgaaattac gctggggaaa tatcaagacc
    18901 agcttttacg gttagaggat aaattggaaa gtggcttgta ttgtgagcta accgacaaaa
    18961 cactgcatga cggttatatt gaatataccc tgctttatga tatgatagca aaccgcatta
    19021 ccattgatga agtacgggca gaaaacggct gtcttagact gatgaaaaat cttgtctggg
    19081 aatatgacgc actccctcac gccctgattg ctggtgggac tggtggcggt aagacttact
    19141 ttctgctgac gctcattgaa gccttactgc ataccaacgc tgtcctttac atcttagacc
    19201 caaagaacgc tgaccttgca gatttgggaa cagtcatggg aaacgtgtac cacaccaaag
    19261 aagaaatgat agattgcgtc aattcctttt atgagggcat ggtacaacga agtgaggaaa
    19321 tgaagcaaca ctcgaactac aagacaggcg aaaactacgc ctatctggga ctgccaccct
    19381 gctttcttat ctttgatgaa tatgtggctt tttttgaaat gctgggaacg aaagaaagcg
    19441 tgagcctact tagccagtta aagaaaatcg ttatgctggg gcgacaagca ggttatttcc
    19501 ttatcgttgc ctgccagcgt ccagacgcaa agtatttctc ggacggtatc agagataact
    19561 tcaatttccg tgtaggattg gggcgtatca gcgagttagg atatggtatg ctgttcggtt
    19621 ctgatgtgaa aaagcagttt ttccagaagc gtatcaaggg tcgtggctat tgtgatgtag
    19681 gaacaagcgt gataagcgaa ttttacacgc ccttagtccc gaaaggacat gattttttga
    19741 agactatcgg ttctcttgca caagcaaggc aggacgggac ggcgacgtgc gaagcgaaag
    19801 gcgacggcac ggactagccg ttgctggtgt ggcgtaagcc acgccagcag gtaaaacccc
    19861 tcggtatcta acagaggggt acgtttgcaa atagacaata gacaaaaaac ataggaaaca
    19921 taaggcttct ggcatggttt cctagcaaaa atcggttgtg aagtcgcccc taaaaacttc
    19981 acactttaca gattgggggt atggttctga atgaagaaca atggataaaa gaattacggg
    20041 agaaacggat tgcttacggt atctcacaag gcaggctggc ggtggcttct ggtataacaa
    20101 gggaatatct caataagata gaaagcggaa aaatgaagcc gtcaaaggaa cttctggaaa
    20161 ctctgcataa ggaactggca aggttcaatc cagaagcacc gcttaccatg ttgtttgatt
    20221 atgtaaaaat tcgttttccc acgctggata tacagcacat catcaaagat atattaaaac
    20281 tgaatatcaa ctatatgctc catgaagatt acggacatta cagctatacg gagcattact
    20341 ctttagggga catctttatc tatacgtcgg ctgacgaaga aaaaggtgtc cttttagagt
    20401 taaaggggcg tggttgcagg cagtttgaaa gttacctgtt ggcacagcaa agaagctggt
    20461 atgacttcct catggacgca ctcgtagacg gtggcgtgat gaagcgtatc gaccttgcta
    20521 tcaacgacca tacgggcatt ttggatattc cagagcttgc ggaaaaatgc aggaaacggg
    20581 aatatatcgg aaagtccaga agttataagt tttaccagtc gggcgagctt atcaagcaca
    20641 gagaggacga cagagaatat atgggacgca ctctttatct tggttcgctg aaatcagatg
    20701 tatatttctg tatctatgaa aaggactatg agcagtacgt caagttgggt acaccgctta
    20761 atgaagccga cattatcaac cgttttgaga tacggcttag aaatgagcgt gcctattatg
    20821 cagtacgaga tttgctgacc tattatgacg cagaacagac cgccttttct atcatcaacc
    20881 agtatgtgcg gtttgttgat gaagaaccag acaagcgaaa aaatgactgg aaactcaatg
    20941 accgctgggc ttggtttatc ggcgataaca gacagagctt gaagctgacg acaaagccag
    21001 agccttacac cttagaccgt acattgcggt gggtacaaag gcaggtagca cctaccttga
    21061 aaatgctgaa aaagattgat aagggaaacg ggactgacta tatggaaacg attgaaaagc
    21121 aggcaacttt gagtgaaaag catgaaatga taatcaaaca gcagacgacc cctgcaaaag
    21181 atttggtgga aagttaggag tgataaaaaa tgtgggaatt agtaaaagac ttcctgctgg
    21241 tatctatggg aatgggtatc ggcgtagtct tgatgtgtat tttgagtatc ggcaaagaag
    21301 ctgactatga aatgaaacaa ttagaagaaa gcgaggacaa ttaaatgaat ttcggacaaa
    21361 atttgtatca atggttttta tcaaatgcac agagcttagt gcttatggcg attgtggtta
    21421 tcggtatcta cttagggttc aagcgtgagt tttcaaaact tattggcttc ttggtagttg
    21481 cccttattgc tgtcggctta gtattcaacg ctggcggtgt gaaagatgta ctgttagagc
    21541 tgttcaataa gattattggt gcataatgat ttttattgta gagcctatat atgaatggta
    21601 taatctgagt atgaaaacaa taaactaagg tttattggat aaggagggat attatgaaat
    21661 tgtgtattgt attatctcca tacaaggctt aatggaatag acttgcatgg agtgaaatgt
    21721 tatccagaga gccttgcatt ttactgaatt tcaaaatgta aaggagaatg gacatggaat
    21781 ataaatatac aaaaattgat ttagaaaaat ggaatagagg taaattgttt caaagttaca
    21841 ttgatgatat gcgtatcgtt atgagcttaa ccgtagatat tgatgtaaca aatctaattg
    21901 aattttcaaa aagccatgat ttgaaatttt atccagctat gatatgggtt gtttcaaagg
    21961 tcgttaatga acatgatgag tttaaatata gttgggacaa taaaggtaat ttaattaaat
    22021 gggactatat ttccccgtcc tatgctgaat ttcataaaga agatgagaat ttcacaaaaa
    22081 tggttacaga tttttctgat agcatatttg aatttcatga acgatttatg ttagatagag
    22141 agaaacataa aatggaacgt gcattcgtag caaatcaacc tttgaacttt tttgatgtat
    22201 cttgtttgcc atgggtaaaa tacaatcatt ttgatgttca tgtttttgac gaagggaaat
    22261 ttcttgcacc agttattact tggggtaaat acgaattaga gcgagaaaga tatataatgc
    22321 ctcttacaat gaatatacat catgccgttg cagatggttt tcatttatcc agatttttta
    22381 atgaagtaca agaattgata aactcaattt cataatctaa taccagatat acacaaagca
    22441 gttagtctta ggattgactg cttttttctt gccaaaattt cagattggag tgatgaaatg
    22501 aacgatattt ttaaggatat gcaggcaaaa gtcggctgtg aatacatttc cgacctgccc
    22561 tcttacaagc gtaaagtgtg gcatgaaatg aaacgactga accctgccga ttatgaagaa
    22621 aaacagttag aagatttttc aaagtatgtg tttggtatgt cgtaccagac cataaaagat
    22681 gtgaggaaac aacagaaagg acgtgaggaa caatgcagga aacaagggtg ctggtggaaa
    22741 cgagaggaac aactggcgaa gaaacaatat catactggtt tgacctgccg atagatgttg
    22801 ccgagtttga agaaaaatta ggtgtcggtg cagaaagtgg ggattaccgc attatcgaaa
    22861 aggtactgcc ttatgctgat gaagtccacg aacacaccag cgtgtatcag ctcaacgaat
    22921 tagattttat gtaccgccag ctttcaagtg atatgcagga agaatatgta tcactactta
    22981 ccgtgtatga gaatttagaa gcactttata tttgcaggaa cgtgattacc gtttatcccg
    23041 actgcaaaag catgattgat gttgcaaggc aaaagctgat gaacgacccg acattcaaac
    23101 atttatccga ggactgtcaa gaatactatt ttgactttga agcctatgct tctcacttgc
    23161 aggaacacgg aaaattttta gtaacggaac acggtatctt tgaactgcca gagtaggaaa
    23221 tgaggtggtg cttatgattg atgatatggc ggtttatatt gctaatcttg gcagatacaa
    23281 tgagggatat ttagtcggtg cgtggttcac tttccctatt gacgaggaag atgtaaaaga
    23341 aaaaatcggc ttgaatgaac agtacgaaga atacgctgtc catgataccg acaactttcc
    23401 tatcgacata ggggaatatg tttccattga agaactcaat gaaatgtatg aaatgataga
    23461 ggaacttccc gactatatcg tagagtgtct ggacgaattt atcagccact atgggacgct
    23521 ggaagaagtc gtggaacata aggacgatat ttatttttat cccgattgtg aaaccatgac
    23581 agatgttgcc tactactaca tagacgaatt gaacgcactt ggggacattc cagctaactt
    23641 acagaactat attgactatg aagcctacgg gcgagatttg gatatgggtg gtcgattcat
    23701 tgaaacaagc cgaggtatct gtgagatacc atattaacgc tggaagatca gcaacaagca
    23761 ttgttgctac tgacagcgta tttctcttta agggacagtc tgaaaatggc tgtccttttc
    23821 tctttgaaga acttacgaaa ggggctgaaa gaacttgaaa aagattaaaa gctacacggg
    23881 tatctggaac gtggaaaaag tcttatatgc aatcaatgac tttaacttac cctttcccgt
    23941 tacctttaca cagattacat ggtttgtgat tacggaattt atcatcattc tgtttgggga
    24001 tatgccccca ctttccatga ttgagggagc atttctcaaa tacttcggta ttcccgttgc
    24061 tctcacttgg tttatgtcgc agaaaacctt tgacggaaag aagccgtaca gctttttgaa
    24121 atcacagata acctatgcct tgcgacctaa aatcacttat gcaggaaaag ccgtaaaact
    24181 gcataagcag atattgaatg aaacaatcac ggcagtaagg agtgtgaact atgttcccga
    24241 taaaatatat tgacaataac cttgtctgga acaaggacaa tgaggtgttc gcctactatg
    24301 agctgatacc gtacaactat tcttttctat ccgcagagca gaaatttatc gtgcatgaca
    24361 gttttcgaca gctaatcgca cagtcccgtg agggaaaaat tcatgcgttg cagattgcca
    24421 cagaaagctc cattagaagt atgcaggagc agtcaaagaa actggtaaca ggcaaacttc
    24481 gtgaggtggc agtccaaaaa atagacgaac agaccgaagc gttagtatca atgattgggg
    24541 acaatcaagt ggactaccgc ttttttcttg gctttaagct catggttacg gaagaacagc
    24601 tcaatctgaa gaacatcaaa aaatcggcat ggctgacgtt caaggaattt cttcatgaag
    24661 tgaaccacac gctgatgaat gattttgttt ccatgccgaa tgatgaaatc aaccgttaca
    24721 tgaaaatgga aaagttactg gaaaataaaa tctcccgtcg ctttaaggtg cgtcgcttgg
    24781 aaatccatga ttttgggtat ctcatggaac atctttacgg cagggacggt atcgcctatg
    24841 aagattatga gtaccagcta ccaaagaaga aattgaagaa agaaacgctg ataaaatact
    24901 acgaccttat ccgtccgaca aggtgtgtga ttgaggaaag ccagcggtat ttacgattgg
    24961 aacatgagga caaggaaagc tatgtgtcct attttaccgt caatgtaatt gtcggggagt
    25021 tggattttcc gtcctctgaa atcttctatt tccagcaaca gcaattcaca ttccccgttg
    25081 atacttctat gaatgtagaa atcgtggaga accgtaaggc attaacgaca gtccgcaaca
    25141 agaaaaagga attgaaagat ttggataatc acgcctatca agcaggaagt gaaaccagct
    25201 caaatgtggt ggacgcttta gacagcgtgg acgagctgga aaccgattta gaccagagca
    25261 aagaaagtat gtataagtta agctacgtca tacgggtgtc tgcacccgat cttgacgagc
    25321 tgaaacgccg ttgtgatgaa gtcaaggact tttacgacga cctcaatgta aaactggtgc
    25381 gtcctgctgg cgatatgttg ggacttcatt ctgaattttt acctgccagc aagcgatata
    25441 tcaatgacta tgtgcagtat gtaaagtcag acttcttggc tgggcttggt tttggtgcga
    25501 cccagcagtt aggggaaaac acgggtatct atatgggcta ttccgttgat acgggaagaa
    25561 atgtgtacct gcaaccgtct ttagcttcgc agggagtaaa aggcacagtt actaatgccc
    25621 ttgcttctgc ttttgtggga agtcttggcg gtggaaagtc attctgcaac aatctacttg
    25681 tatattatgc ggtgctgttt ggcggtcaag cacttctgtt agaccctaaa agtgagcgtg
    25741 gcaactggaa agaaacgctc ccagagatag cccatgaaat caatatcgta aaccttacca
    25801 gcgacaagga caatgcagga cttcttgacc catttgtgat tatgaagaat gtgaaagacg
    25861 ctgaaagtct ggcaatcgac atcttaacat tccttacggg tatttcctct agggacggcg
    25921 aaaaattccc cgtgttacga aaagcggtgc gttctgttac ccagagtgaa aatagaggac
    25981 tgttacacgt tattgaggaa ttgagaaaag aagataccgc tatctcacgc aatatcgcag
    26041 accatatcga cagcttcacg gactacgact ttgcacacct gctgttttca gacgggacgg
    26101 tagaaaatgc tataagcctt gataaccagc tcaatatcat tcaagtagca gaccttgtac
    26161 tgccagataa agataccact tttgaggaat acacgaccat tgaattattg tcggtgtcta
    26221 tgctgattgt gattagtacc tttgcc
//
LOCUS       DACXMZ010000028        26092 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.028, whole genome shotgun sequence.
ACCESSION   DACXMZ010000028 DACXMZ010000000
VERSION     DACXMZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26092
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.028"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            39..776
                     /gene="cas6"
                     /locus_tag="JD016_04685"
     CDS             39..776
                     /gene="cas6"
                     /locus_tag="JD016_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HAU4998926.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGKERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREFYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            781..2256
                     /locus_tag="JD016_04690"
     CDS             781..2256
                     /locus_tag="JD016_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998927.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIVKNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     VNTKNLGKKMIDNGNLSNLKELLLDNISFLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2276..3289
                     /locus_tag="JD016_04695"
     CDS             2276..3289
                     /locus_tag="JD016_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HAU4998928.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYIGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINGRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            3305..4102
                     /gene="cas5"
                     /locus_tag="JD016_04700"
     CDS             3305..4102
                     /gene="cas5"
                     /locus_tag="JD016_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HAU4998929.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4129..6537
                     /locus_tag="JD016_04705"
     CDS             4129..6537
                     /locus_tag="JD016_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HAU4998930.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINNILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNKNVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDIDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            6978..7925
                     /locus_tag="JD016_04710"
     CDS             6978..7925
                     /locus_tag="JD016_04710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAU4998931.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            7944..8702
                     /locus_tag="JD016_04715"
     CDS             7944..8702
                     /locus_tag="JD016_04715"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAU4998932.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8704..10002
                     /gene="mtaB"
                     /locus_tag="JD016_04720"
     CDS             8704..10002
                     /gene="mtaB"
                     /locus_tag="JD016_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HAU4998933.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKNINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10045..10395
                     /locus_tag="JD016_04725"
     CDS             10045..10395
                     /locus_tag="JD016_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HAU4998934.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10558..10737
                     /locus_tag="JD016_04730"
     CDS             10558..10737
                     /locus_tag="JD016_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAU4998935.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10767..11210
                     /locus_tag="JD016_04735"
     CDS             10767..11210
                     /locus_tag="JD016_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAU4998936.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11321..11818
                     /locus_tag="JD016_04740"
     CDS             11321..11818
                     /locus_tag="JD016_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAU4998937.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12011..12574
                     /gene="raiA"
                     /locus_tag="JD016_04745"
     CDS             12011..12574
                     /gene="raiA"
                     /locus_tag="JD016_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAU4998938.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            12781..13023
                     /locus_tag="JD016_04750"
     CDS             12781..13023
                     /locus_tag="JD016_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HAU4998939.1"
                     /translation="MLMLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIK
                     TKVKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13036..14379
                     /gene="yqfD"
                     /locus_tag="JD016_04755"
     CDS             13036..14379
                     /gene="yqfD"
                     /locus_tag="JD016_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HAU4998940.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14417..15433
                     /locus_tag="JD016_04760"
     CDS             14417..15433
                     /locus_tag="JD016_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAU4998941.1"
                     /translation="MIIQKKFTIADGNFERELFGNFDENVKLIEKTLNIDVILREGNI
                     ILIGEEKNVDSALKLMNELHQTVSNGKHLDKQSISYSLSLLLEGSEQKIKELEGTIVI
                     TQRGKAVQPKTLGQKEYIKLIENNDITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRII
                     LTRPAVEAGESLGFLPGDLKDKVDPYLRPLYDALFEMLGADKFNKYLERGTIEVAPLA
                     FMRGRTLDNSFIILDEAQNTTSEQMKMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLI
                     QATEILKGVPGIGSIMLTDRDVVRHELVQRIIRAYEKHDKREEFKKEERKKAKMQEKT
                     FKRK"
     gene            15490..15951
                     /gene="ybeY"
                     /locus_tag="JD016_04765"
     CDS             15490..15951
                     /gene="ybeY"
                     /locus_tag="JD016_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAU4998942.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            15957..16676
                     /locus_tag="JD016_04770"
     CDS             15957..16676
                     /locus_tag="JD016_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAU4998943.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            16795..17196
                     /locus_tag="JD016_04775"
     CDS             16795..17196
                     /locus_tag="JD016_04775"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAU4998944.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17260..18153
                     /locus_tag="JD016_04780"
     CDS             17260..18153
                     /locus_tag="JD016_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAU4998945.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18156..19544
                     /gene="mgtE"
                     /locus_tag="JD016_04785"
     CDS             18156..19544
                     /gene="mgtE"
                     /locus_tag="JD016_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAU4998946.1"
                     /translation="MGYMDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFD
                     IMENLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDM
                     ADKLSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEA
                     IDHMREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVD
                     EDREEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHE
                     REVAEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFI
                     PVVIGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVM
                     RKLDIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVY
                     FIITTALLSKIV"
     gene            19617..19772
                     /locus_tag="JD016_04790"
     CDS             19617..19772
                     /locus_tag="JD016_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HAU4998947.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            19738..20511
                     /gene="recO"
                     /locus_tag="JD016_04795"
     CDS             19738..20511
                     /gene="recO"
                     /locus_tag="JD016_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAU4998948.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20501..21103
                     /locus_tag="JD016_04800"
     CDS             20501..21103
                     /locus_tag="JD016_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HAU4998949.1"
                     /translation="MMSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYL
                     ENKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIP
                     LTVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMIT
                     NAAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21263..22141
                     /gene="glyQ"
                     /locus_tag="JD016_04805"
     CDS             21263..22141
                     /gene="glyQ"
                     /locus_tag="JD016_04805"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAU4998950.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22141..24207
                     /locus_tag="JD016_04810"
     CDS             22141..24207
                     /locus_tag="JD016_04810"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAU4998951.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFDKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKIKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(24373..25920)
                     /locus_tag="JD016_04815"
     CDS             complement(24373..25920)
                     /locus_tag="JD016_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HAU4998952.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
BASE COUNT        10675 a         2579 c         4693 g         8145 t
ORIGIN      
        1 agaataatga attatactag aaagtaggag gtgaaaatat gagcagaatt tcaattttta
       61 tggaatcatg taatggcaaa tttccaaatg aaaattcaat gttgtcagtt tcctttataa
      121 aaaatatttt aaatgcagaa aacaaagatt ttgctaaaaa catatttaac tatgggaaag
      181 aaagaaaaaa caataagcaa ataaaggata taaatacagc catttacata ccaaatctta
      241 taagaagcaa aaatgagtta cttgttgatg gagatataat attcaatata agtttttata
      301 attattctat gttcagtaaa ttatacaatg gaattttaaa agtaaaggag ctagaatata
      361 aaggttatag atttaaaata aaaaatataa aaatacataa agaacacgag ataaaagaaa
      421 atggtgttat atttaaaact atgtctccta taatagtaaa gaataaagaa ggtaaatatt
      481 tagatgtaga agattcaaat tatatagaat gtttaaacta tatagctaat ctaactctgg
      541 agagtataag aggtagtggg cttagaaagc cattggaatt tatacctctt aacttcaaaa
      601 aaagagtgtt aaaagaaaag attcgtggat ttaaagtgag agaattttac tatataaatg
      661 cttatgcagg aacatttttt ttaaaaggag atatggaaga tttaaatgca ctgtataaaa
      721 tgggaattgg atatagaagg acggaaaatg caggaatggt tgatatatta aagtagcgag
      781 gtgagagaaa taaaagctaa attttataga tcaggtgata ttgtaagaga tattggcatt
      841 gtttactttt atgaattact aatggattta aaaaacgaac tagaaaaaaa tgattataag
      901 ctaaaattta cttgtgagtt aaatagaaat tatttatctt tagaatctgc agaatatata
      961 gaccctaagt atatatctga ttatatatta gaaaatcaac tttttaaggt atttaaaaat
     1021 gggaaaaagg aaaccttaga aaaaatattt agtaatatag ataatttaac gtatgtaaat
     1081 tttattgaag aattggataa atcttctgca actgctaaac aaaaggaaga tattaaaaaa
     1141 gattttaaaa acaggaggtt tccatatgtt agaaatagtg gtaagtatgg attaaacacg
     1201 agtttagaaa atatggaaac aaatgtaaaa agaatagtag atactgtaat taaatcaaat
     1261 atagagatag atgaaataga taaacttgat ttgacacaat atgaagaaaa agatagtgta
     1321 tgtaatgttt gcaatataaa taaaacaaca aaactagata ttgatttaag agaacgaaag
     1381 gactctaagt ataacttttt atttagaggt gcagaaaaat caggctttaa aagaagtgga
     1441 caagttgaaa gtaatatttg ttttgagtgt gaatttttca acttaatgtg tcttttatat
     1501 ataaatttaa aaagaccaat ggtttttgca tatacgaatg atttaagaga attagcattt
     1561 ttaaatcata agataatgct taaaagaaaa atgtattcag ataaatcatt ttataaaaaa
     1621 cttttgcatg aaaaaataag ttcaattaga ctatatagat ttgatattgt taagaataaa
     1681 ggtataattc taaagtttga ctctattata gagtataaag aattattaaa aaaaattgaa
     1741 cttatagata ttatagataa ttataatttc tcaagggagc cagtaaacac taaaaattta
     1801 ggtaaaaaaa tgattgataa tgggaattta tcaaatttaa aagaattatt acttgataat
     1861 ataagttttt taaagcaatc aactggaact tctcgagaaa tggattttgt atctagctca
     1921 tataatatcg gtttatatat aaaattgtgt tggattgtag atggagggga ggagtataaa
     1981 atgaaaaact atatggaaag taatttaata tattcaagag taggtaaaga cttatttaat
     2041 aaaatgacag atgaatccag aagaaaaaat ttttctatga aagtgattca actacttaag
     2101 tctaatgata gaactcagct atttcaaact ataatgcatg tgttagtttc taatggaatc
     2161 ttaataggag aagggtttgt agaaggtcta atgcagagtt ctgaattaga attaaatacg
     2221 aatgtaggtc tatttattca agaaatcatg aaataaaaaa gggggaaaat ttaaaatgaa
     2281 tgaaaaaaga gtaaaaggag taacattaac tatactgaca gaaagcccag ttccactttc
     2341 atatgaccaa gggtatggaa attatacacc aataaaaaaa gaacaatata gagaaaaaat
     2401 acatgcaaaa actagtatag ccacaataac atatgattta agaagaatgc tccaccaaga
     2461 atatggttgg aatttaagta atattgtttt tggaaaaaaa ggaaatatat atccatctat
     2521 taagaaaaat gtaggatgtg tagatgaaaa tggattagag actgatgtat ttggttattt
     2581 gataccactt aaagataaag gttctataag taaagcttct ccacttagaa taataccttt
     2641 taggtcatta aatccatata taggttcaac acagctaata acaaatagag gttttttaag
     2701 tagcgaattt ggtagaaaat attatgatga gaaggaagaa aatgaagtac ctagagatga
     2761 aaactttcca acaacacaag cattagcaat agaagagact ttatcagatt actacgttta
     2821 tacaataaca ttggagcttg atagaatagg agtagtagaa gtagaagatg ggaagttact
     2881 tttaccagaa gaaagaaaat ttatgagtaa ggaattaaga gaaaaggcag taaaagatat
     2941 attagatgca attactgtgt ttacaagaaa cataaaacat tcatcagtac tattaaaacc
     3001 acttgcagta ataggtggag cttttgataa agttgtacca tttttttggg atgatgtaga
     3061 ttataatgct gatagtggtg agataaattt agagggagtc atagaaacta tagaaagtta
     3121 tagccttaaa gaaagtaaca caatacttgc tatcaatggc agattaaaaa tttctaataa
     3181 aaaggaatta aataagttta atttaagtaa ataccctgta aaagagatta aaaatctagc
     3241 agatagattg gaaattggag aagataatat gtggtattta aaagagtagg aggttttttg
     3301 aattatgaaa gtattacaat gtaaacttaa gcaaccaact gctcactata gagacccaaa
     3361 agtatttcaa aatgaatata taagtacttt aaatttacca tctaagacca caataatggg
     3421 aatgataacg tatttatgtg atagaagatt aaactctgat atagatatag gaataattgg
     3481 aacacatcat catagagaac tagaattttc aagaggagag aacattgatt tttggaatga
     3541 atatacaaat atgagaaaag gcaaagataa agaaaagttt ttgcttcaag gaaactacta
     3601 tgattactat aaagaacata aagctcaaaa tagcatatta aactatgaag ttcttaaaga
     3661 agtagaactg actatattta tttcttgcaa ggatgatgaa gaacttgaat ttattaaaaa
     3721 aaagcttgaa agtccatgta aatatgcaaa tttaggaaga aaagaagatt ttgtaatacc
     3781 aagtgaaaaa gggtgttttg tgaaagaagt tgagttaaaa gaggttatac ctatgaatac
     3841 tcgtgatgct ataaaagaaa atataaaatt aaagaataca tatgtaagag ttgacttaag
     3901 agataaagag aatgtcgaga ctataataaa tcaaggtgta ttgattgcat tacctcataa
     3961 atataaagat ttagaagcaa atagagatga tagagtttat gagttttgtc attatatata
     4021 tgttgataat gatggtatat atcctaaaaa tattaaggta aatgtatgta ttgatacaaa
     4081 agaagttttt acatggcttt aaattatttt gaaagatagg tgtttatcat gaaaaatatc
     4141 atgcaaaaag aaatattagc taagtcatac aatttaagaa ctaatcaaca agagcaaaca
     4201 ttagttgaac atattaagga tttatttgaa gtattagaat ctatattaga actaaatcta
     4261 tattcagata aagatgtaga gatattgaaa atatgttgtg ctttgcacga tttaggaaaa
     4321 attaattcaa ttatgcaaca aaaaatggag attaataata aaatatcgta tagttgctca
     4381 gaagaagaga ggaaaaagtt agagtctgac aaaaaatctt taaaaaagat agcaagacat
     4441 aatatatttt ctggagcatt tttaaaagat atattagaaa aaatgaactt gtcagaagaa
     4501 gataagttat atatatataa aagtataatg ttacatcatg gaaactatga agattatatg
     4561 aggttaagta ttagtaaagt tcaagaagaa atttatgagt atattgaaaa aggcatttta
     4621 gaaaaagaag agtttaatct gaaagatata gaaagttata taaataacat tttaaatgta
     4681 gactttaaat ttggtgaaga tgtattggat tatgattaca tagataaatt aagtgaaagt
     4741 atgtttatag acagtgatta taacaatggt caaatagatg attcagtttt aaattataga
     4801 aagtttaagt atattttata taaagggatg ctgaacttaa tagaccatag tgcatcaagt
     4861 agacagaaag gtataaaatt ttataacgat tttactgatg aagaaattga taatatgatt
     4921 ttaaatgaga tatataagag tcagggtaat ttagaaaaaa ataatataga gttcaatact
     4981 atacaaaaaa ggttgagaga actttctgga agaaatgttc tgactgttgc atttacaggt
     5041 tcaggaaaaa cagctgcaga ttatagaaag acttttaaaa gaaaattctt tttagtgcca
     5101 aataaaatat cagcagaaag tttttataga aaaaatattt ttcaaaataa aaatgattta
     5161 gatacaagaa gtagtaatga ttacattgga ttacttcatg gagatataaa cttatattca
     5221 gagaatgaag ataatggaga acatgatttt gtattgacat taagggatat agatttgagt
     5281 ataaactttt gtaaaccttg tgtaattgca acagttgacc agttattact tagtatgttt
     5341 aaatttccgg gatatgaaaa aatattcgca gccgtaaaaa atgcatctat aactgttgat
     5401 gaagtacatt tattggaacc aaagatgttt ttgattttga tatattttat gcagtttgca
     5461 tgcaagtact tagatgttga ttttcactta atgacagcta cacttccaaa atcatataaa
     5521 gagcaaatga taaacaaggg aattattttt caagaagaaa gtaataaaaa tgttactgat
     5581 aaaggagaaa tagtttttat tgagagcaac aaggaagaag agatttgtga aggtaaagat
     5641 gtaaaggttt cttttataaa ggaaaaagaa atcaagtcaa tagttgaaga tgctttagaa
     5701 aataaacaaa aaattcttat aataaagaat actattgatt cagtcaatag aacatatgag
     5761 tttttaaaag aaaatttatc tgataagtat ggtggtgtag atatagatgt tttacatagt
     5821 agatttaagt tcaaagataa aaaagagaaa tatagtaaaa tattaaatgg agagggtgat
     5881 atttggatat caactcaatc agtagaaata tccttggatt tggatttcaa tataataata
     5941 agtgatttag caactatgga tagcttgata caaagaatgg gtagatgcaa tagaaataat
     6001 aaatatgaat atggaaactt ttatattcta cctagtgaag ataaaattta tgataacaaa
     6061 cttaagaaca caactaaaaa catcttaaag gacatattaa aaactaactc tatttttaca
     6121 atgggaatta gaaaaactat tttggatgat tactatgata atagtgtagt caaaaaatat
     6181 tttgaagaga attttataag ttgtgataaa gaaattaaga atatttatgg tataaataaa
     6241 gaaatttttg atggattaga tttaattttt aattttgaac cttacaaaaa tattgttgat
     6301 agtaagaagg aagcagctaa aatttttaga gatattgatg ttagttacaa aattattctg
     6361 gaagaagatt tttataagga agatagggag ttacaacaag attcgattca agtatcaggg
     6421 tttatattta atagattata ttattatgga ttaataagta aagtagaagg atacatggta
     6481 ctaaaatcta gtagtaagtt tgagtataat agtactgtag gcttaatttt gaagtgagca
     6541 tatgtttaaa tataatatac ctttagccat tgaaaataca aaaagtacta ctgtaagtcc
     6601 actgtaaaaa ctatttttta aaaggaaaaa taagttgtga gatagagtgt ttacaagtgt
     6661 atatagataa taaatttgag gtttaagatt aactaagtgg gtttcaggta catcgcgaaa
     6721 ataattataa gttggagatt ttgtatgttt aagattaact aagtggattt caaatattga
     6781 tatagtagat ataatagaag ttttattatt aggtttaaaa ttaactaatc ggatttttaa
     6841 atattctgac tattatataa ggatattatt agcttgaatt taaaaccaat aatataaaac
     6901 aacaaaatat tatatttatg atataataaa agttatggaa aaacaggaat attactatag
     6961 agaagagagg taaacttatg aataattgga tagaagtaac tataaaaaca acaacagaag
     7021 cagttgaacc tataacgaat atactatatg aacaaggagc tggaggagct gtaatagaag
     7081 acccaaaaga ctttttattc caaaagaaaa atgaactaga ctgggattat gttgaagaag
     7141 aagtatttaa aaagaatgaa gaagatgatg ttcttataaa aacttatgtt tctgaagaaa
     7201 aaaatgtgat ggaatttgtc gaaataataa agcaaaaagt tttaggatta aaagattttg
     7261 gtatagatat aggtgaagga agtgtatcat tggaccaagt aaatgaagct gattgggcaa
     7321 atgcatggaa agcatactac aaaccaacaa aagtaggaca aagggtagta gtaaaaccta
     7381 catgggaaga ttatgctatg caagaggggg atttaatcat agagcttgac ccaggaatgg
     7441 catttggtac agggacacat gagactacta gtatgtgcat tagagaatta gaaaagtatg
     7501 tgaataaaga ttctaaagta tttgatattg gctgtggtag tggaatcctt gcaatagcag
     7561 cagctaaatt aggagcaaaa gaagttgtag ctgtagattt agatgaagtg gctgtaaaag
     7621 ttgcaaaaga aaatgttctt gaaaataaag ttgaaaaaag tgtatctgta atgcatggta
     7681 atttaactga tgtaataaaa gataaagcgg atgtaatagt tgcaaatata atagctgata
     7741 taataaagat acttgcaaaa gatgttcaaa actttatgaa agaagatgct atattcatat
     7801 cttcaggtat aattttggat aaagtagaag aagttaaaga aagcttaatt gaaaatggat
     7861 ttgaaatagt tgaagttcaa aaattaggtg aatggtcagc aatagtttct aagcttaaaa
     7921 aataagggat atggtgacgt ataatggata gattttttgt tgaaaaaaat aatataaatc
     7981 tacaagataa aacttgcact atagaaggtg aagatgtaaa acatatttca aaagttttaa
     8041 gatgcaaatt aggtgagaaa cttgaaatat gtgacaagaa taataatgag tatatttgtg
     8101 agataatgaa tatagataag agtatagtta atttagagat tttagaaaaa gttgatataa
     8161 atagagaatc agaacttaaa gtaaggctat accagggact tccaaaagct ccaaaaatgg
     8221 aaatgatact tcaaaaatta actgaagtgg gtgtagaaga aataatatta gttcaaacta
     8281 agagaagtgt tgttaaagtt gatgacaaaa aagaagataa aaaatttgaa cgttgggaaa
     8341 gaataattta tgaagcagca aagcaaagca aaagaggtaa gataccaaaa ttaagaggtg
     8401 ttttaagttt taaagaagct ttagaagata tgaaaaaaaa taatgtaaat atttgccctt
     8461 atgaaaatga aagaacagtt tcaataaaac atgcactaaa aaaatgtgat tctaatatag
     8521 atagtgttgg aatttttata ggtccagaag gtggattttc tgaagaagaa atagaacaaa
     8581 tacaaaaaaa taattgtaat gttgtatcat tggggcctag aatattaaga actgagacag
     8641 cttctgttgt agcatcaaca atagctttgt atgaactaag tgatttagga ggagaaaaat
     8701 aaattgaaaa aagtagcttt ttatacactt gggtgtaaag ttaatcaata tgaaacagaa
     8761 gctatgctag aactatttga gaaagatggt tatgaacaag tcaatagtga agagtatgct
     8821 gatgtttatg ttataaatac atgtacagtt actcatatga gtgatagaaa atctcgtcaa
     8881 tatataagac gagttaaaaa gaagaatcca gatgcaataa tagccgttgt aggatgttat
     8941 tctcaggtat ctccggaaga aattttggat atagaagaag taaatttagt catgggtaca
     9001 aatgatagaa gaaagattgt tgaagaaatt aaaaatataa attcaagtaa gaaagttagt
     9061 acagttgatg acataatgaa agttaaagct tttgaagaaa tagaaatcag ccaaactaat
     9121 ggaaagacta gagcatttat gaaaatacaa gatggatgtg acagattttg tacttattgt
     9181 atcattcctt atgcccgagg acgcgttaga agtagagata ttgatagtat agtcgatgaa
     9241 gtgaaaaaat tggctaataa tggctataaa gaggttgtat taacaggaat acatgttgcc
     9301 tcttatggaa aagatttaaa ggatagggat attaaacttc ttgatgtaat aaaacaaata
     9361 aaccagatag aaaaaataga aagaattagg cttagttctg ttgaaccaat tttatttaca
     9421 gatgaatttg taaatgaagt tttaaagatg gataaagttt gtcctcatta ccatttatca
     9481 cttcaaagtg gatgtgatga aactctaaaa agaatgaata gacgatacac aactttagag
     9541 tacaaaacta ttgtagatag attgagaagt aaaatgccag acgtggcaat aactacagat
     9601 gtaatagtag gttttcctgg agagacaaat gaagagttta aaaaaacgta cgagttttta
     9661 aaagaaatag agctttcaca aatgcatatt tttaaatatt caccaagaaa aggaactcca
     9721 gctgcaacta tggaaaatca agttgaccct caaatgaaac actttagaag tgaacaactt
     9781 ttaaatttaa gtaaagtaaa ttttaataaa tttgcaacta aatttatcgg ccgtgagttg
     9841 gatgttttat ttgaacaaaa tatagaaggc aataaatatg agggattaac ttcaaattat
     9901 ataagagttg ttgttgaaag tgacaaaaat atacaaggtc agatattaaa agtgaaaata
     9961 aatgatgtaa aagatgaata tgtagaagga attttgctat aacatgtaga aattataata
    10021 tttataagat aggaggtgga aagaatggat tgtatttttt gtaaaatagc gaacggagaa
    10081 ataccttcaa ctaaagttta tgaagatgat agagtcttag cttttaatga cttgaaccca
    10141 gttgctccat atcatatatt agttgtacct aaaaaacatt atgatagttt aattgatatt
    10201 cctgataaag agatggacat tgtgtcacat attcatgttg ttattaataa aatagctaaa
    10261 gagaaaggtt ttgaccaaac tggattcaga gtcataaata attgtggaag tgatggaggg
    10321 caagaagtta aacatttgca ctatcatatt ttagctggta aaaagcttcc taattacgaa
    10381 gcgggacaaa actaaactaa attattgaaa aattgagttc cttcttgata aaaatacttg
    10441 ataaaagtaa aaatttagtt tataataaac aaagtgttag gttgtaatgc atattatgca
    10501 aaaagaacat agttacagtc ccagcataat tgcgctattg gagggaggga aaaagaaatg
    10561 tcagaagtaa gagttagaga gaatgaaaca ttagacagcg ctttaagaag attcaagcgt
    10621 caatgtgcaa tgtctggcat catgtcagaa gtgagaaaaa gagagcacta tgataagcca
    10681 agcgttaagc gtaagaaaaa agctgaagca gcaagaagaa aaaacgctaa gaaatagtag
    10741 taaataagaa taaagaggtg aaggggatgt cccttaagca aaagttacaa gaggatttga
    10801 aatcttcgat gaaaaacaaa gatacagtga gaaaatctgt agtaacttta ataagagctt
    10861 ctataaagca gtatgaggtt gataataggg tcgagcttga tgaagatgga attatcgatg
    10921 taatagcaaa acaattaaag caacgtagag atgcattagt tgaatttgaa aaagctggaa
    10981 gagaagactt aataaaagaa acagaaggtg aaattgaagt tttaaaagag tacctacctc
    11041 aacaattaag cgaagaagag ttagaagaaa ttgtaaaatc tactatatct gaagtagggg
    11101 caacttctat gaaggatatg ggaaaaataa tgtcagttat ccaaccaaaa gttaaaggaa
    11161 gagctgacgg aaaacttata aataagttgg taaaacaaaa cttacaatag attgatatag
    11221 cctagagaga aatctctagg ctttttatag tttttgggaa taacgagtca tatttgagca
    11281 tatctataat caaagaaaat tttgaaagaa ggggaaaaat ttgattatat cagtaaaaga
    11341 gattaggaat tttttgataa acatatttat accactaata ataggttact taagtagcat
    11401 cttaacaatg attatcagtg gaacagatat ttcaacctat tatttacaac tcacaaagcc
    11461 aagctttgca ccaccagctt ttatctttcc tatagtttgg actatattgt acatattgat
    11521 gggtatatct tcatacctaa tattaagaaa gggttacaac ttacctaaag taagagatgc
    11581 aattttttat tatggattac aacttgtatt aaactttatt tggagtatac tattttttgg
    11641 atttggactt agatttacag ctttgataga tataataata atgattttgg tttctataat
    11701 aatgatttcg aagttttcta aaatagataa gagatctgga tacataaatt tgatttattt
    11761 aatgtggctt gtttatgcag gatttttgaa ctattttata tggtttatta ataaatagaa
    11821 tgtaaagatt ttgtaaatta ccctgtactt ttaataatat cataacaaac gaaaaataaa
    11881 tagactatta aaaataatca ttttcatata caataatatt aatttaaata caataaattt
    11941 ataaaaattg tatttaaaga ttaacatatt aaagtattag aattggaaag gcttgaaggg
    12001 agaggttttt atgcaaataa tagtatctgg gagacaaatg aaattaactg acggaataaa
    12061 aggatacgta gatggaaaat taagtaggtt agaaaaatac cttgatcctg aatcagaagt
    12121 gaaagtaaca gtaagtgcga aaaaggatag acaaaaagtg gaggttacaa taattcctat
    12181 taacggtcaa ataataagag cagaagatgt agaagatgac ttatatgcag caattgatat
    12241 tgtttgtgac aaattaagta gacaagttgt caaatataaa actaaagtta aagacaaagt
    12301 tcaaaataat aagagcataa gatttgaaaa tcttgacttt atagataata gcagtgaatt
    12361 tgatgattat gattatgatg atgaagaaga tgaaaatata gtaatagaaa gaagaaagaa
    12421 atttaatgta aaacctatga gttctgagga ggcgatactt caaatggaac tagtaggaca
    12481 caatttctat atgtttagaa accaagataa ttttgaaata aacatagtgt ataagcgtaa
    12541 agctggtgga tatggtttaa tagaacaaga ttaataaaaa ttgtatacgt ttagatttta
    12601 tataagaaaa aataaaaagg taatcatgtt gattttttat caatatgatt acctttttct
    12661 tgtttaaata atatattaga tattaggtat ttattatata aaaattttaa aactaatctg
    12721 ttacaacatc aataaagtgc ataaatacct atttcaagca taaaattaat taagggggta
    12781 atgcttatgt tagaaatatc cactgattta caagtaaatc aaccagttat tactgttaca
    12841 agtaatacgt ttattagtat agaaaattat ctatcaatat tagaatatga agttgattta
    12901 ataagaataa agactaaagt taaaacaatt aaaatatctg gggataaact ttcacttaag
    12961 tatattactg atagtgagat aggtataaaa gggattatct acaatgttga gtatgttgat
    13021 taggaggaga tacacttgat aagttttata aggggatact atgttatagt tgtggaagga
    13081 gtaggactcg aacaattttt aaatcatctt ataagaaatg ggataagtgt ttacaatgta
    13141 actagaataa aaaatactaa aatggagttt catatagata gacaagatat aaaagacttt
    13201 aagaatgttt atagaggaag taagtttgat ataaaggtaa agcaaaaaac tggggtccct
    13261 tttattatca aaagagttta taagcacaaa ggtatgtgga tttgtgctct tgtttcttta
    13321 tttttattga tgtctacatc gcagtttgtg acagatgtat atatacaatc tcctgaaggt
    13381 ataaaaaaag aagctcttag aaatgagctt tataaggttg gagtaaggcc aggagtctat
    13441 aagaagagta ttgatagaaa agaagttaga gaccatatga tgtcaaaatt taatgatgtg
    13501 gcatatttat ctatcaatgt aaaaggaaca aatatatttg ttactgttac aaaaaaggca
    13561 gaatccctaa aatctacaga ccaatcaaat tattgtaatg taattgcttt aaaaaatggt
    13621 ataatagaaa aggtaatccc tagaagtggt aaatcagtta ttaaatctgg agacatagtc
    13681 caaaagggag atgtcttatt aaatggagct aataccaagt caataccaga agtatgggca
    13741 agtacttttt atgagtccac aaaaaaggca agttatgtag atactgtaaa taaaaaaact
    13801 ggagaaaaga aaaatatata tacactaagt ttttatgata aagagtttac aatgagaaaa
    13861 aatataaaat ataaagatta tgttgttgaa aataaagaaa aaaaactatc aataggaaac
    13921 tatacttttc caataaagat aaaaacgagt actttttatg aaacaaaaaa ggttgaagta
    13981 aaaaggaata aagaagagtt aaaaaaagaa ttatctgaaa aagctttaaa agagttagaa
    14041 tacataatcc cagcttcggc tagaataata gatgtaaaac ataactttaa ggttaataaa
    14101 aatatgttag aatatttaat aacagttcaa actagtgaaa atattgctaa aatttatcct
    14161 cttagtaagt cagaagcaga aaggtttata aaggaagaaa gtaaaccaga tgaaggtgaa
    14221 gaggaagttc cttccaatcc agaaaaaaga ccattaaatg atataagaaa tgagtttgat
    14281 gaggataata aggataataa agaccaaaac aattctgatg aaaataatag taatcaaaat
    14341 agtaataata gtcaaaataa taatagcaat aacaactaat aaaattaaac ttaaaatctt
    14401 aggaggacta attcaattga taatacaaaa gaaatttaca atagcagatg gtaactttga
    14461 aagagagtta tttggcaact ttgatgaaaa tgtaaaatta atagagaaga ctttaaatat
    14521 agatgtaata cttagagaag gcaatataat tttaattgga gaagaaaaaa atgtagattc
    14581 tgcattaaaa cttatgaatg aattacacca aactgtatca aatggtaagc atttagataa
    14641 gcagagtatt tcttattcat tatctctttt gcttgaagga agtgaacaaa aaataaaaga
    14701 acttgagggg acaatcgtca taactcaaag gggaaaagct gttcaaccta aaactttagg
    14761 tcaaaaagaa tatataaaac ttatagaaaa taatgatata acttttggtg ttggacctgc
    14821 tggaacagga aagacttatc ttgcggttgc tatggcagta aaagctttta aaagagatga
    14881 agttagtaga attatactta caagaccagc tgtggaagca ggagagagtc taggtttttt
    14941 accaggagat ttaaaggaca aagttgaccc atatttaaga ccactatatg atgccttatt
    15001 tgaaatgttg ggtgctgata aatttaataa gtatttagaa agaggaacta ttgaggttgc
    15061 accacttgca tttatgagag gtagaacact tgataattca tttataatat tggatgaagc
    15121 tcaaaatact acttctgaac aaatgaaaat gtttttaact agacttggat ttggctctaa
    15181 agctgtagtt acaggagatg taactcaaac agatttacca caaaataaga agagtggtct
    15241 aatacaagct acagagattc ttaaaggtgt tccaggtatt gggtctataa tgctaacaga
    15301 tagagatgtt gtaagacatg agttggtaca gagaataata agagcttatg agaagcacga
    15361 caaaagagaa gaatttaaaa aagaagaacg taaaaaagcg aaaatgcagg aaaagacttt
    15421 caaaagaaag taaaagtgta gtaatataga tatatagcat tattaatgaa taaaaagtag
    15481 gtgtagtgaa tggatttgat actagatgat agacaagata aattagaagt aagtgaggag
    15541 ttaattgaaa agataaagga tataatcata gaatgtttag attatgaagg atatgatgat
    15601 aattatgaag tgagtctttc ttttgtagat aataaagaga ttcatgaatt aaatagagaa
    15661 tatagaggag ttgatagagt gactgatgtt ttatcatttc ctcttttaag tgatgatttt
    15721 gaggatgttg agttagaaga agaatcccta ggtgacatag tagtatctct tgaaagagcc
    15781 ttagaacaga gtatagagta taaccatagt tttgaaagag aagtatgttt tttaatatgt
    15841 catagtatgt ttcatctttt aggttatgac cacgatacag atgaaaatac aaaagaaatg
    15901 agagaaaaag aagaacacat attaaataag ttaaacataa caagggagta gtttttatga
    15961 aaccagaaaa aactcgtcaa ggtataatta aagcatttaa tgcagctata gaagggatat
    16021 tgtatacatt taaatttgaa agaaatatga aaatacatta tttaggttca gttgctgttc
    16081 ttataataag tttgtttttt aacttttcaa aattagaaat gataatgtta cttatgtcca
    16141 tatgtttagt tgtagttgct gaaatgttta atacagctat agaaaaagct gttgatttag
    16201 taactgatga atatcatgtt cttgctaaga tagcaaaaga tgttgcagct ggaggcgttc
    16261 tagtggcagc actaaattca gtagtagttg gatatatttt gttttatgat aaattgacag
    16321 atatatctgg aatattaatt tataaaataa gagaatcaga attacatata accttgatat
    16381 gtatattatt ggttcttata gctgttgtag ttgtaaaagc attgacttct acaggtactc
    16441 ctctaaaagg aggaatgcca agtggtcatg ctgccttagc atttgctata gcaactgcaa
    16501 ttacacttat gactgaaaga gttgttgcct ctactcttgc atatataatg gctgtattag
    16561 ttgcccaaag tagaattgaa ggaaaaatac ataccttttg ggagactatt gctggagctt
    16621 tattaggtgt gttgatagct atattagtat ttcagcttgg aatgttctat aattaaaata
    16681 aatgtacata aaatatttaa aataaaacaa atctgtgcta attaaatttt tatgatataa
    16741 tgtagagtat taaataaaaa taacttttaa ttattgtata caggaggagt tgttgtggat
    16801 aacatagaat tgttaaggtt ggcagaagat gccagacaac attcttatgc tccttactca
    16861 ggttttagag taggtgcagc tcttttaact aagagtggta aagtttatac tggatgtaat
    16921 atagaatgtg catcattagg tggaacaaat tgtgcagaaa gaacagctat ttttaaagca
    16981 atatcagagg gtgataagga tatctataaa atagctatag caagtgataa ctcagaaaat
    17041 aatgagcaaa catatccatg tggaatatgc aggcaggtaa ttatagagtt tggttctgat
    17101 ataaaaataa taacgggata tactaaagga gaggtgtttg agcatagtat aaaggatttg
    17161 ttgccaaact atttctctgg aaatgattta aaataaattt aaaagtataa ataaatttaa
    17221 gtaattaaaa caagaagata aaaagttagg agacagttaa tgttcaaatc aggttttgtc
    17281 agtatagtgg gtagaccaaa tgtaggaaaa tctactttaa tgaataatgt agttggagaa
    17341 aaaatagcta taatgagtga taaacctcaa actacaagaa atacaataca agcagtatat
    17401 acagatgaag aaatgcagat agtattctta gatacaccag gtattcacaa accaaaaaat
    17461 aaattaggtg aatttatggt aaaggcagca acagaagctt ttaaaaatgt agatttaata
    17521 ttatttgtag ttgatgattc taaaaaaata ggtcctggag atagaaagat aatagaagat
    17581 ttaagaagtg ttaagacacc aataatttta gttgtaaaca aaatagacca actagaccaa
    17641 aaagatgagc tatttgatat aatcaaaatg tatgatagag aaggtatttt taaagaaata
    17701 gtaccaatat cagcattaaa aggaaagaat acggatacct taataaaagt tatacaaaat
    17761 tatttagaag aagggccaaa gtattttcca gattatatga ttacagacca gccagaaaga
    17821 gtattaatag cagaacttat aagagaaaaa gttcttcact atcttaatga tgaaatacct
    17881 catggtgtag ctgttgaaat tgaaaaaatg aaagcgagaa atgataagga aatagtagat
    17941 gtatctgctg taatatactg tgaaagagat tcacataagg gaataataat aggaaaaaat
    18001 ggtaggaaac taaaaggaat aggtaagtca gcaagacaag atatagaatt gcttttaggt
    18061 tcacaaataa atcttcaatt atgggttaaa gtcaaagaaa attggagaaa tttacaaaat
    18121 tatataaata attttggata taatgataaa taaaggtggg ttatatggat aggaaactgg
    18181 atgcttccca agatttatta tatgaagtaa aatcattaat tgataataat aaagtattgg
    18241 agttaagaga attaatagag gaatatcata taattgatat attcgatata atggaaaatt
    18301 tagaggaaga catgaaaatt caattatttg aagtacttcc tttagatatg gcatcttcta
    18361 tattagaaga aggtagtgta gagtttttta ttagtatttt atctaaactg gatgtagaac
    18421 actcgaaaaa tatacttgaa cttatgtctc ttggagatat ggcagacaag ttgagtgaac
    18481 ttgaagaaga agaaagagaa cacataataa atcttttgaa tcaagagaat gccgattatg
    18541 taaaagagtt actattctat gatgaagatt ctgctggtgg aacaatgacc actggatata
    18601 tttctataaa taaagatatg acagctcttg aggctataga ccatatgaga gaagaagctg
    18661 aagaagctga aactatttat tatatatatg tggttgatga tgaagaaaaa ctagttggag
    18721 tattgtcatt aagagaatta ataatagcta gagacgcaaa tatagtagaa gatttgatga
    18781 gtgaaaatat aatctctgtt tatgtagatg aagatagaga agaagcagta agattagtat
    18841 ctaaatacaa tttaatagca attccagtag tagacagaca agaaaaatta aaaggaataa
    18901 taacagtaga tgatataatt gatgtaatgg aagaagaagc aactgaagat atgtataaat
    18961 ttgcaggaag ttcagaacat gaaagagaag ttgctgaaaa agaaaaccct acattgagag
    19021 agcaaataat atcagcactt agaggacgat taccttggtt aattattacc ttagttggag
    19081 ggttgttggc ttcgttaata ctttctaact tagactatat aatgaatcct gtatatgctc
    19141 cattagtatt ttttatacct gtagtaattg gtatgggtgg aaatattgga actcaatcat
    19201 catctgttac agtaataaca ttatctaata aggatttaaa ttttagcaat gtggttagag
    19261 aaggtattgt aggtattatt acaggtcttt tgtgtagtat aataacaggt attgtaattt
    19321 actttgttat gagaaagtta gacattgtat taattgtttc aatatcatta tttataaata
    19381 tggttttagg tgcaacaata ggtgcgttta tgcctgtttt attaaaaaaa atggatgcag
    19441 acccatctac agtttcatca ccaattatat ctacagctct tgatataact ggtatagcag
    19501 tttactttat aataacaacg gcattattgt caaaaattgt ttaataactt tatgtatgat
    19561 ttatcctctt cttactaaaa atataagtat aaatgtaaga agggggtttt ttaaaaatga
    19621 acaatatcag ttgggaagtt tttaaaaaaa caggtagtat agatgcatat ttatatttct
    19681 gtgattgcaa aaacatacat gaagaaattg aagaagtaag ggagaaaaaa gaagacgatg
    19741 ataatcctga acacccaggg gatagtactt aaagctataa ggtataagga aagtgacata
    19801 atacttacac tatttacaag aaagcttgga aaagtatctg caatagcaaa aggagctaaa
    19861 aaaaataaga gctctttgct ttcatcatca cagttatttt cttatagcaa ttttacctta
    19921 aaaaaacaag gtaatatgta caaggttact caaagtgaaa taataaaaag tttttataat
    19981 atatcctatg atatagaggc attttcttat gctacatata ttacaaaatt agttgaaaat
    20041 tctatacttg aaaatcagac taataatagg ctatttatat tacttgcgca gactctgtat
    20101 ttgtatacac aggataatac agacaatagg tttataacag cagcattcga gttaaaattt
    20161 ttagactata taggatttaa acctatagta aataaatgca ttaattgtga atgtaaaatt
    20221 ttacaaaatt ctgtgtttaa tatctacgaa ggtggtatac tatgtagtaa gtgtagtaaa
    20281 ctttttgata ataatataaa attagactta acaactataa gtcttatgga atatgtatta
    20341 agaaatgata ttttaacatg tagtaaagct aaagtatcaa aatatataac acatgaactt
    20401 gagaatattc tagacaagta tttgaaagtt tatgttgata atattaattt taaatcatta
    20461 cacgtattgc aaagtgtaaa aaataataag ggagtggata atgatgagta gtaatataac
    20521 tattgaaatg gttgattcag tctttgaaag agttccagga gcaacttatg cagaagcaaa
    20581 agaagcatta gtaatgtgca atggagatgt aattgaggca gtaatatatt tagaaaataa
    20641 aaaaaatact tgtaattgta aagcaaaatc tgctaaagaa actatggaag aagtatttgg
    20701 caaagatgga gaaaatataa agtttcaatt aaaagagctt cttagaaaaa gtagtgtagt
    20761 gagaataata gttgaaaaag atggaaaaac tatgatgaac attcctttaa cagttggagt
    20821 tgttggatta gcttttgtac cattagctac tttagttgga ttatcagctg ctgttataag
    20881 taaatatcgt ataaaagttc aaaatgaaga agatggagaa attgtagatt taggtgaatt
    20941 gaatgaagaa aaacttcacg ttttgaagaa tatgataact aatgcagcaa aagatgtgaa
    21001 ggatgtagta gttgacaaaa aagaagatga caaagatgta actgctgacc ttatgaaaga
    21061 agaatctgaa aagaataaag aagacgaaga cgaaaataaa tagatataaa gtgactttta
    21121 aaaaatgaag agaagataat tataagaatt atcactaaca ttgacaaatt ttatagaatt
    21181 tgatattcta aaagttgtag aagaactatt aaaatagcct ttcgtgtaga cgagaggctt
    21241 tattgttata ggagggattt ttatgaattt tcaagagatg atactggcgt tgcaaaaata
    21301 ctggtcaaag caaggttgta tcatgatgca accttatgat attgaaaaag gtgcaggaac
    21361 tatgaatcct aatacttttt taagatcatt aggacctgag ccttggcaag tatgctatgt
    21421 agagccttca agaagaccag cagatggaag atatggtgaa aacccaaaca gactatacca
    21481 acatcatcaa ttccaagtta tactaaaacc ttctccagat aatatacaag aattatattt
    21541 ggaaagttta aaagagattg gaattgaccc aagtgagcat gacataagat ttgttgaaga
    21601 taactgggaa gctgctacag ttggtgcttg gggacttgga tgggaagttt ggctagatgg
    21661 tatggaaata actcaattta cgtattttca acaagtaggt aatattgaat gtgagcttga
    21721 gacaggagaa attacatatg gattagaaag attagctatg tacattcaag aagttgatag
    21781 tgtttatgat ttaaaatgga atgataaaat aacttatggt gaagtattta ataaagctga
    21841 gtatgaaaac tcaatgtacg catttgaatt atgtgatgca gatatgttgt ttaatctgtt
    21901 tgatatatat gaaaaagaag cattaagact tatggaaaat ggacttgtaa taccttcata
    21961 tgactatgtg ttaaaatgct ctcatgcatt taatacattg gatgcaagag gtgcaatagg
    22021 agttagtcaa agagcttcat ttataggtag agttagaaat atggcaaaga ctgttgcaga
    22081 aacttttgta aaacaaagag aagaaatggg attccctctt ttaaaggacg gtgacaaata
    22141 atgaaaaatt atcttttatt tgaaatcggt gttgaagaaa tgccatcaag atttgtaggc
    22201 agtacactag aacaattaaa aaacaatctt agtaaattat ttgatgaaaa tagaatagct
    22261 tttgataaaa taaatgcgta tgggacacca agaagactta ctttagtagt cgaaggattg
    22321 agtgatagac aaaatgattt agaagaggaa ataaagggtc ctgctaagaa aattgctgtt
    22381 gatgcagaaa ataatttaac aaaaccagca ttaggattta taaaaagcaa aggattaagc
    22441 gaatctgaca tatattttaa acaagttgga aaagaagaat atatatttgc gactataaag
    22501 caagatggaa aagaaacttc agaagtacta aaagacatat taccagagac tataaaatct
    22561 gtagtattcc caaaagctat gcgttgggga ggaaaaaatc ttaaatttgc aagacctata
    22621 agatggatag ttgcattgtt aaatgataaa gtattagagt ttgatttaga aggaataata
    22681 tcttctaatg taacaaaagg tcatagattt ttaggtgaaa gtacaataga agttgattct
    22741 atagaagatt attttgaaaa attagaaaaa aattatatag ttttagacca aaacaagaga
    22801 aaagaaatga taagaaaaca atgtatagaa gtagcaaatt ctttaggtgg agaagttgaa
    22861 tttgatgaag atttacttga agaggtaact tatttagttg aatatccaac tgctttttat
    22921 ggagaatttg atgtggatta tataaaacta ccaaaagaag ttgttataac tcctatgaaa
    22981 caacatcaaa gatattttcc agtacttaaa gaaggaaaac tgttacctaa ctttatagct
    23041 gttagaaatg gtgattctta cagaatagac aatgttaaag ctggaaatga aaaagtttta
    23101 gaagcaagac ttgcagatgc tttatttttc tatagagaag atactaagag aaacttagaa
    23161 agctacatag aaaaattaaa aactgttgta ttccaagtaa aactaggaac tatttacgac
    23221 aaaactttaa gacttgaaaa attaagtgct gatatacttg atagtttagg tttagtagat
    23281 gaaaaggcag atactataag ggctgctaaa ttatctaagg cagatttagt tactggaatg
    23341 gtaggagaat ttgatgaatt acaaggtttt atgggaaaag aatacgcaaa agttggagga
    23401 gagaatgatg ttgtaagtga agctattttt gaacattatc ttccaagatt tgcaggagat
    23461 atacttccta agactaatgc aggagttgcc ttatcaatag ctgataagtt ggattcaata
    23521 gcaggtttct ttgctatagg aatacaacca acaggttctc aagacccata tgctttaaga
    23581 agacaagctt taggtatact tagtatactt atggacagaa aagttgctgt ggatataaaa
    23641 gtacttattg aacatgcttt agacaattat tctgaattag attttgataa agaagaagtt
    23701 gttgagcaaa taatgaattt cttcaatgag agagtaaaaa atctatttaa agatttagga
    23761 ataagatatg atgttgttga tgctgtttta agttctgata taaatgatgt ttctgatatg
    23821 catatgagag ctttagagct taataattgg ctagaaaaag atgaattagt ggagatgctt
    23881 actgcattta atagagtttc tactctagct caaaaagcag aaagtgataa aattgatgaa
    23941 tctttattaa aagaagatgc tgaaattaaa ctttacaatg aatttaaaca agtaaagata
    24001 aaagttaatg aattattaaa ggataagaaa tatggtattg ctttagatga atttgcatct
    24061 ttaagaccag tagttgataa tttatttgat acagttatgg ttatggataa tgatgaagct
    24121 attaagaaca atagacttgc tttacttaaa caagtatatg atactatgct agaaatttgt
    24181 gatttatcta aaattgttta taaataaaat ttaaactatt tataagaaat aaggctgtct
    24241 caaaatagag atagtcttat ttttcattgt tgagatgatg aaaatatatt tgatttcata
    24301 gaaaaagagt gtctcataaa ctaaaaagtt cattttgaga cacccatgtt ttttgtaagt
    24361 aaatataata aactatattt tatacttatc agttattttg tttaaaatat tatcatttga
    24421 atttacccat tcataaaaat tgcctgatga tgcgtccgat attttataaa gcatttcagg
    24481 aatatcacta gctttaaaat ctccaagtga ttttcccaat ctagtcttac ctatttcaaa
    24541 actgcctcca acaaataatt caaatgcatc tgtagatata ccatcaacat tcttcttttt
    24601 accacataaa cctatgctac caacttgatg aacaccacat gaatttaaac atcctgatat
    24661 atataactta ggtgctttat ttaaaatttc ttcattattt tgtaatctaa aataatctat
    24721 gatttcatgt aacattttct gactattttg tatgcccatt tgacaaattg gaacacctat
    24781 gcaagatata ctttgttcta attgagaatt acaactaata gttttactta tttctaatat
    24841 tctttttgct tcatttccat ttaaatttaa aatatataat ccctctgtca ttcctagtct
    24901 aatcatagga ttttcaacat tatctaattc tttaagtaaa gtacttaaat cctttgttaa
    24961 caaaatacct ccaactggat gtatatatac agtgtataaa ccatcctgtt tttgaggtat
    25021 tagtctagaa tcttgtatat ctattttaac tccaggttta gagtaatcta tttcttcaat
    25081 attgagttca agactaccat tttctttttc taatttatag tattctttaa atttttctaa
    25141 gaatagttct tcaccaagtg attcgaccat atatctaacc ctagctctgt ttttattttc
    25201 ataatttcca taatccataa agaatttaat cataccttca acacaatata aggcatcttt
    25261 cggctttata agttcatcaa gttctaaagc aactttgggt tgtttaccta gacctccacc
    25321 taagaaaact ctgaaatatg gttcatcatt tttcaaagta gcaacaaatc ctaagtcttg
    25381 tatactacaa tgagcagtat ctgtgtaaca attagaataa gaaactttta attttctagg
    25441 taaatggtat gtagtagcat ttttgataaa atatttatca gtagcaactg catatggagt
    25501 aacatcaaag acttcactag ggtcaactcc agataatgga gataatccga catttctagg
    25561 ataattacca ccaccacctc tagtgaatat atcatttttt attccttctt ccatgatatc
    25621 aacaattgaa tttatagata aatcgtgtaa ttgtatagct tgtctagttg ttaagtgtat
    25681 cttatctaag ttgtgtttaa tagctatttc atagatttta ttaagttgat actgagataa
    25741 aactccagca gatactctaa gtcttatcat aaaagatttt ttgtctcttt gagcataaac
    25801 accatatcca ccagaaaatc ccttatattg cattttattt atttctccat ttaaaaattt
    25861 tgaagaatat tctttaaaat taggtagttc atttaacaat atctttttaa aattatccat
    25921 tatttcacct cattgttagt cttgtataaa aatatatcta ttgtataata gatatatata
    25981 ttattatcta gtcaggataa aaatataaca gaataattct attaaaagta agaataataa
    26041 ggacatagtg agtattttgt ttttatttta caaattaagt ataatatttt tg
//
LOCUS       DACXMZ010000029        26046 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.029, whole genome shotgun sequence.
ACCESSION   DACXMZ010000029 DACXMZ010000000
VERSION     DACXMZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26046
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.029"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(28..840)
                     /locus_tag="JD016_04820"
     CDS             complement(28..840)
                     /locus_tag="JD016_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HAU4998953.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            1261..2412
                     /locus_tag="JD016_04825"
     CDS             1261..2412
                     /locus_tag="JD016_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU4998954.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRSHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTKDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVNFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            2530..3168
                     /locus_tag="JD016_04830"
     CDS             2530..3168
                     /locus_tag="JD016_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HAU4998955.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            3169..4191
                     /locus_tag="JD016_04835"
     CDS             3169..4191
                     /locus_tag="JD016_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HAU4998956.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLDCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            4181..4801
                     /locus_tag="JD016_04840"
     CDS             4181..4801
                     /locus_tag="JD016_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998957.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(4925..5611)
                     /locus_tag="JD016_04845"
     CDS             complement(4925..5611)
                     /locus_tag="JD016_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAU4998958.1"
                     /translation="MNTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(5625..6170)
                     /locus_tag="JD016_04850"
     CDS             complement(5625..6170)
                     /locus_tag="JD016_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU4998959.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            6398..7645
                     /locus_tag="JD016_04855"
     CDS             6398..7645
                     /locus_tag="JD016_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HAU4998960.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMSNIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            7732..9726
                     /locus_tag="JD016_04860"
     CDS             7732..9726
                     /locus_tag="JD016_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAU4998961.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            9726..11048
                     /locus_tag="JD016_04865"
     CDS             9726..11048
                     /locus_tag="JD016_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAU4998962.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDEISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            11327..12613
                     /locus_tag="JD016_04870"
     CDS             11327..12613
                     /locus_tag="JD016_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAU4998963.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGILYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            12699..14006
                     /gene="grdB"
                     /locus_tag="JD016_04875"
     CDS             12699..14006
                     /gene="grdB"
                     /locus_tag="JD016_04875"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:13746..13748,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HAU4998964.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            14308..15015
                     /locus_tag="JD016_04880"
     CDS             14308..15015
                     /locus_tag="JD016_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4998965.1"
                     /translation="MISIAICDDESYMLNHLKENIELYMKQKNLLYNIELFNKVENLL
                     CNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAFEVDAINYL
                     IKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVIKRKIYVHL
                     KKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGDRIPVSRLR
                     QQIFSKVMLEFMKNRRI"
     gene            15017..16375
                     /locus_tag="JD016_04885"
     CDS             15017..16375
                     /locus_tag="JD016_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAU4998966.1"
                     /translation="MINLFSSDLFLKYSFFFSFIVYRILMVIVQYYFLINFTNFNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            16465..16899
                     /locus_tag="JD016_04890"
     CDS             16465..16899
                     /locus_tag="JD016_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998967.1"
                     /translation="MKVGNINGQAPITNGLDSCQSEVQGLEQKKKSLQDEIEKVRSDK
                     SIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEENNVSEVNNNLLERKKEPS
                     NNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRPI"
     gene            complement(17162..17398)
                     /locus_tag="JD016_04895"
     CDS             complement(17162..17398)
                     /locus_tag="JD016_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998968.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSGHLLIVHNGI
                     VIHNKFLILNDFGNKNGVSISEGYQPTYKIQTAS"
     gene            complement(17553..17777)
                     /locus_tag="JD016_04900"
     CDS             complement(17553..17777)
                     /locus_tag="JD016_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAU4998969.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(17874..19127)
                     /locus_tag="JD016_04905"
     CDS             complement(17874..19127)
                     /locus_tag="JD016_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HAU4998970.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNDFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            19387..20376
                     /locus_tag="JD016_04910"
     CDS             19387..20376
                     /locus_tag="JD016_04910"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAU4998971.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHEKAYNLVKDKKILLELCPTSNVQTKTVDSYEVHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            20448..21188
                     /locus_tag="JD016_04915"
     CDS             20448..21188
                     /locus_tag="JD016_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HAU4998972.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            21388..22539
                     /locus_tag="JD016_04920"
     CDS             21388..22539
                     /locus_tag="JD016_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HAU4998973.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            22564..23319
                     /locus_tag="JD016_04925"
     CDS             22564..23319
                     /locus_tag="JD016_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HAU4998974.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            23511..25898
                     /locus_tag="JD016_04930"
     CDS             23511..25898
                     /locus_tag="JD016_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HAU4998975.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPSPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVDDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
BASE COUNT        10388 a         2807 c         4333 g         8518 t
ORIGIN      
        1 ataaatataa aattataaaa tttttattta tttaattttt tcaacaacat tagtcaatat
       61 tgaatctaaa tcatcactaa tttctgtaac tccattatga gtaatatcta taatttcttt
      121 agtcatagga agttctccta aaagctctaa gtctaaatca tctaaaaact tttcagtaga
      181 ttctccttca aataacttga tttttttaga acaatcagga cattggatat aactcatatt
      241 ttcaataact ccaaggacat ttatgttcat ttttttagcc atattaacag cttttgatac
      301 aatcatagat accaaatctt gaggtactga aaccataact attccactta taggaataga
      361 ttgcataact gttaaggcaa catctccagt accaggaggc atatctatta ataaatagtc
      421 taattcttcc caaagtacat cagtataaaa ttgctttaca acccctccaa gtaaaggtcc
      481 tctccaaaca acaggttcat tttcatcatc tatcataagg tttatagaca ttacttttat
      541 gttatttgag ttttctacag ggtaaatata agagccatca gaataagctt ttacattttt
      601 tacacccata agtctaggaa tacttggtcc agttatatct gagtctaata taccaacctt
      661 atatcccatt ttgtttagct tattagcaag taaagcagtt acagttgatt ttccaactcc
      721 acccttccca ctcataacac caattatttt tccaaattta ttgttagggt tattttcaat
      781 agaacaattt gattgactat tacaatttcc ctttgatgga catgaattac aatctgccat
      841 atctattacc tccaaacttt aatatttatt agattatttg taacctacat atactatttt
      901 agtacaattg aaccataaag tcattattta aaagcaactt agaagatttt ctattgattt
      961 attcatagac tatagcaaaa agaatacaat aaaaaaatta taacattctt gaaaaagtga
     1021 ttatttcaat ttagttatag aacttagtca tataaatata tagaaaatat tttaatatat
     1081 tatacataac gggttatttt ataaatttaa ctataaatta ggtttatatt ttataaaaat
     1141 ttgctaaaaa aacaattaaa aattgtaaag aaaaaagata taattagaac tgtagtaata
     1201 aatgttttat tatagataat agagttttaa gtatcttaaa taatatttgg aggaatatga
     1261 atggaattaa gaccaagaag tgcaaaatta gaagaatttg ataaagtaat agaattaata
     1321 aactatgtat ttagaatatc aagaagtcat aaaccaacta tgatggaaga atttcctctt
     1381 ttactaagta aaaataatat agaaaatatg attataatat cagaagatga taaagtggtt
     1441 tctgatgtaa actatctaat tcaagatgta tctattcaag gaaacagatt gaaggttgca
     1501 gctataggtg gagtatgtac tcatccagat tatgaaaaga gaggctattc atctaagata
     1561 ttggataaag tagaagaaaa aatgttttat gatggtgtag atattgtaat tatatcagga
     1621 acaagaagtc tatacagtag aagaaattgt agtttggtaa agagttttta taaatataca
     1681 ataaaacctg aagatgtaaa aatagcatat gaaatagttg aatttgatga aactaatttt
     1741 gaaaaagata atgatttaga taaaatgatt gaactatata atcaaaatag tactagattt
     1801 ataagaacga aagatgagtt tcaaaaacta ttgcatgcag caactattgc ctggggacca
     1861 attggttaca agaaggtatt tataaaggaa aataataata taataggata tttgataata
     1921 agaacaataa aaaaagaaga ttctactgta ggagaagttg ctgaaatagg gttaaactct
     1981 gtaaatgttg aaaatatatt gaaatatgtt gcaaataaat ttggattaga atatttaaat
     2041 tataaagtac atgtgaaaaa tcttaaagac cagttgaaat gtaatgggac aaaatctttg
     2101 gactatcaac aaggaactat gaaaataata aattttacta agttgtgtga tagtttaaga
     2161 agttatttta gtcaatatgt aaattttgaa ttactaaaat acatggaatt taaacaagtt
     2221 gaaaataaat atattattaa atacaaagaa gaagaacttg taatagaaaa tttggataag
     2281 cttaataaac tgttttttga aaaaaatgag gaacagtaca atgaatttaa acatctaaaa
     2341 aatatatatg aatttgctac aaaggcattt cctgtagatt ttccatggac tgcaaattta
     2401 aattatcaat agttttagat gatattaaat aaaattgatt gatacctaga gttagtcctt
     2461 gttaaataag agaaattatt ggatattgaa aaagcaattt tgtaagtaat tagaaaggat
     2521 gagtattata tggacatact tgatgatata gcagaatgta tattaaatat gggtgtagat
     2581 aatatagaga atttagtaaa aattgctata gataaaaata ttgatgtgga agatatttat
     2641 gaatatggtt taaacaaagg tatgatagga gctttagata agtttgagaa taaggaatac
     2701 tatctttcag aagttatagt atgcacagat gcattaaata aaggtattaa tcttttgaag
     2761 ggaactggaa aagtaaagaa aaaatcaaag ggagtaattc tgatgtctgt agtagaagga
     2821 gatactcatg agataggaaa aaatattgta aaagtaatgg tagaagccac tggatataag
     2881 gttattgact taggtgtaaa tagaaaaagt gaagatataa tagaagaagc tataaaaaac
     2941 aatgtagata taataggctt atcttcaatg atgacaacta caatggaaaa tatgaaatcg
     3001 gttatagacg aacttaatat gattgagata gataaaagac caaaagtaat aattggtgga
     3061 ggacctgtaa gtatggagtt tgcagaagaa ataggagcag atggatacag ttcaaatgct
     3121 ccaaaagcag ttaaattaat aaataaactt ataggaggtg aagtttaaat gttttcagat
     3181 atgactttac ttcagtatat aaatagtgaa gatagaggtt tttttctgcc tgatatgggt
     3241 acaaatggat tgttcttaat aaataaaaag gcatatgaag tatatgatag tgtagatttg
     3301 caattagaat tagcaaagac tatggacaag tattttgaat cagattttat atactcattc
     3361 tgtgatggga ttacattttg tgaaactctg ggtttagaga cattaagacc agattatgat
     3421 tttccaagtg tattgacaca tactataact gacagagata aattaagaaa attagatgtt
     3481 ccagaccctt atacgagtgg acgtatgcca ctcaatatag agagcttatc agttatatca
     3541 aaaagtatta aaaaaccact atatgaatcg atacaaggtc catttacact tgcaggtcaa
     3601 ttagctggag ctactcaact tttaagatgt ataataactg ataaaaaatt tgtagaagaa
     3661 ttattggaat ttacaactga attggtaaga agatatgctg tagctgcaaa taaagcagga
     3721 gcaaaatata tttcaatagc agagccaaca tcagtaactc taagtaaaga tagatttgat
     3781 gaatttatag taaaaaattt aaataaaatc tacaatgaac ttgattgttg gaaaggtatg
     3841 catatatgtg gagacaccag agaattatta gataatatgt tagattgtaa tatagatgca
     3901 gtaagtttag accaaatttt agactatgaa gaaattgctc caatgattcc tagagatata
     3961 gttttgattg gtaatttaga cccaataaaa cttcttggac gttctaagcc agataagatt
     4021 agaagagaga cgctaaaatt gttaaagaag atgagaagat atgataactt tttatgtgat
     4081 tttggctgta actgcttaaa taccactcca gtagaaaatt tacaagcagc tataaaggca
     4141 ggaagaatta gttataaaga attagatagg attgatattg atgaattata atagtaataa
     4201 aaataaatat cttttaaagg atataaattt agatgatatt tttatagata agaatattgt
     4261 gctaaagttt ttggggtatg gaaatagaaa agctccagat acaattagta aaatcgttga
     4321 aagagaaata agaaatataa atgatatttt agatatcaag atatatataa gtgaagttga
     4381 tattaataaa acacctaaag aatgtaaaaa agcatttgct gtactatata caatagggga
     4441 taaaattgat attaagatga atgactatat ggaaaattgc aatatgatgg cagggctagc
     4501 attggataaa ataggtatag tatgtttaga ttatattaat gagaaaataa aaatgtattt
     4561 aaaaggaaag tatgatagtc tcaaaatatc gcatgaaata tatccaggag ataaagattt
     4621 tgaagtagag agacaaaagg atatttataa ctatgtaaaa aataaatata atagtataga
     4681 aatagaaatc aatgattatc atcaactatc ccccataaaa agtgttgcaa tgcttatttt
     4741 aatgggggat gaagaaaatt tagaatcaag atgtagcaaa tgtcctagaa aatgctttta
     4801 actagttttc aagactaaga tttgttttgt gtatgaattg caataatttt tttggtcatt
     4861 ttatgtataa actaaaaaaa ttattgcaaa gtaatattct atttaatttg aacagactta
     4921 caaactatag tagtttcttt aattcaataa gtgtatttat ttcataagtt ggtatatgat
     4981 tagtataatt tatactgtta ttggaattta accaacaagt atctattcct gaatttattc
     5041 cacctaagac atcagaggtt aagctatccc caattattaa ggcagaagat ttatcatgac
     5101 aattaaactt tttgagagcg tatttaaata tttcaggatt tggttttgat attttcaact
     5161 cttcagatac aaccattcca tcaatatatc cttttatttt tgaattttca agtcttttct
     5221 tttgaactgc agaaattcca tttgttatta taacaatagt atactttttt ttaagatatt
     5281 gtaatatatc gattgcacct ggaattaaaa atgtacattc acctaaaaaa tttaagtaca
     5341 ttttgctaag agtttcagaa tcaacatcca aagatatttt atttgcaaaa agttcaaatc
     5401 taagtaattt aagttcattc aaagttataa tatttttttc aaggtcgagc caaagcttat
     5461 cacttatatt tctatatgac tctatataat tctcaaaatt aaattctaaa tcaaactcag
     5521 atagaagttt tttaaaagca tgtgattcac ttttcttaaa atcaaataaa gtatcatctg
     5581 catcaaaaaa tataaattta tatgtattca tggtttttac tcctttacaa gatgactgca
     5641 aaaattactt tactttcact atttgagtca ttttgccatt tatgttttgt tcctaaagga
     5701 acttttacac tatctccttt ggacaaagta aattcatcat catccataaa aagtttaact
     5761 tctccctcca aaacatatgc aatttcatct ccattatgag taatcctatc aacacaagaa
     5821 gaagtgtttg gagctaaatc cataattgca aattctaaat taccactaga gtctggaatt
     5881 aaaatctcat atataacttc tttgcttcca ggtaaaataa ctttttttct attatcatgt
     5941 cttactatca agtttttttt atctacttca gatacaaaaa atgtaaataa aggaacattt
     6001 agagaagagg ctatagattt taatgaattt acagatggat tagcaagtcc tcgttctatt
     6061 tggctcaata atgatggtgt aacatcagct agttttgcta aatctcgaat acttaagttt
     6121 tgtttttttc gaacttcagc tattttttcc cctaaattta aatcattcat ataaatatcc
     6181 tctcttttta atttaaaaaa tattaactta aaataatcaa gtaaaatgat tatgtagtat
     6241 gaaattatta taacatatag aattaggttt tgaaaaaact atgttcaata taattaaata
     6301 aattaaaata taattgacaa agtggataca gaatacttaa aatatattta aataaaatta
     6361 agcaaaagat agttaattta tgataggagg ggatattatg caaataacga cttttggagc
     6421 tatatttggt ttattaatag caattatttt aattataaaa aaatttcaag ctgtatatag
     6481 tttaatgtta ggagcattta ttggaggatt agttggagga gcaaatataa cacaaactgt
     6541 agattttatg gcaaatggag ctatgaatat atcaccatca attcttagag ctttggcatc
     6601 aggtgtatta gctggaagtt taataaaaac aggggcagta gataaaatat cagaacagat
     6661 tgtaaaaata tttggtgaaa agagggcttt attttcgatt gccatttcaa ctatggtact
     6721 tgctggagta ggtgtaaatt tggatgtatc tataataact gtagcaccta taggattata
     6781 tataggtaga aaattaaatt attctaaatt gtcaatacta ttagctatgt tgggaggggg
     6841 taaagcaggt aatataatat caccaaatcc aaatactata gcagtagcag ataatttttc
     6901 tgtcaatctc tctagtgtta tgatgtcaaa tataattcca gcaattattg gagttgttat
     6961 tacagttatt ttagcaagta tattaataaa taaaggaaac aaagtacaat catatgaaat
     7021 tttagagcaa agagaagatt taccaagttt atttaaatca ttatgtggtc caattatagc
     7081 tatatttcta ttgtttttag gaaatgtatc tcctatagtt atagacccaa tgatagccct
     7141 tccaatagga ggaatcgtaa ctctaattgt cacaggaaac ttgaacaatt caagagaata
     7201 tttagctttt ggattatcta agatgcaggg ggtatgcata ttattacttg gaacagggac
     7261 tatcgcagga attatacaga tgtctgaatt acaacagagt acaataggtg ctttacaatt
     7321 tttaaatatg ccacaatttt tattggcacc agtttcgggt atacttatgt cattagcaac
     7381 agcatcatca acagctggtg ctactatagc atcatctact tttcatgatg ctataataaa
     7441 tggtgggcta tcaccgattt caggtgcatc aatagtaaat gctgggtcta gtgtattcga
     7501 gcaattacca catggctcac tatttcatac tagtgcaggt agtataaaca tggatatagg
     7561 tgaaagattt aagttaattc catatgaagc actgatagga atagtaatga ctataatttc
     7621 aacatctatt caattagttt tgtaataaat ataaatattt aatgaaaaaa gtaggatttt
     7681 gttattaaat attgaattta ttatagtaaa aaagaagatt ggagttttaa agtgaataaa
     7741 aaaaagattg ttattatagg gattatttat tcatttttag tagtattttc acttacaaat
     7801 atgtatgtaa atatggagta taatctaaat gtatttgaat atattaagaa gtcacttcca
     7861 tttacagaag aagaaaaaaa gtggttagaa aaacacaaga atttgattta cagctctgac
     7921 caaagttcac ctcctttgag atataaaggt aaggaagatg gacagtataa gggaattatt
     7981 gttgatttaa taaattctct gtcaattcaa attggtagag atttttattt taaaccaaat
     8041 aattggtgga aagaatcttt cgtaaattca atagatgata gtataaagtt ttttgacttg
     8101 attccatcaa aagaaagggc aaataaattt atatttactg acccaatata cactttgagt
     8161 gcaaatatat tgaaagataa aaagtcccaa gatataaata gttacatgga tttaaagggg
     8221 aaaacagtag caattccaga aggtgattat tctataaatt ttttaaagca aaaaattcaa
     8281 gatataaaca ttttattgac tccagatata aagactggag tcaaccattt aatgtctgga
     8341 aaagtagacg cagttgttgg tgatgaacca gtattgcgat actatataaa taattatgga
     8401 ctttcaaata aatacagtgt tttaagtaat cctatatata ctaaaaaagc agttcttgct
     8461 gtacctaaac aatatgaaga gctggttagt attttaaata aaggcatttt taaacttcag
     8521 aaaaatggag tgtataaaga tttaaagaaa aaatggtatt ctacgtataa tgaagttgat
     8581 gatatattgt atgagcgagg aattgtgcct agtatttatc tttttatagg tataatttta
     8641 atatcaattt atgtttttta tagctataca tatttgttaa agatagaaat caaaaggaaa
     8701 acagaacagg taatagaaaa taaaaaaact ttagaggcaa cttttaatag tattacagat
     8761 ataataatgc ttgtagatga aaataacaat atagtagaat cgaacaaagt actatatgac
     8821 tttatgggtg aaatgagtta taagatagca gatttgatta gcatgataaa aggtgttatt
     8881 gaaaatacct tctctgaaaa tactaataaa actagtgaaa tagaaataca caataaaatt
     8941 ttaaagataa acacttttcc tgtagagtat aaaaaaaata atactgagta tatagtagtt
     9001 cttattaaag atataactaa tgataaaata gtagaggcga aattacttag agaaaacaaa
     9061 atgatatcta taggacaatt agcttctggg gtagcacatg agataagaaa tcctttgggt
     9121 ataataagaa ataattgcta tctcttaaaa gataacgtta caatggaaga agtaaatgat
     9181 tgtgttaaat caatagaaag taatgtagat agggcaagta atataataac caacttgtta
     9241 aatttcgcaa gaatatcaga tgataattta gaacatataa atataagaaa ttttattgaa
     9301 aatatagtga aacttcaata taaaatgtta cagttaaaga atgttgaaat aaaaattgac
     9361 tgtgaacata atttaatatg ctatataaat ggagaatcgt taaaacatgt atttataaat
     9421 ttaatttcta attcaataga tgcaatacat caagatggaa aaattattat atactgttat
     9481 gaaaaaaatc attgtttatt tatagatttt aaagataatg gtgaaggaat aaaagaagat
     9541 gccctaaagg acatatttaa tcccttctat acaacaaaac caattggtga gggaactggt
     9601 ttaggtttat acattactta taatgagatt aaaaagaata atggagatat aagtgttgaa
     9661 agtaaattgg gggttggaac ttgttttcat ataaaaattc cattaaataa agaggtgact
     9721 atataatgaa aatactatta gttgatgatg agctagaata tggagtggta atgaaaaaaa
     9781 ttctacaaaa gaagggctat cttgtagata tagcactaag tggtgaagaa gctataaata
     9841 taattaaaaa agataaaaat tatgatttgg ttttatctga tgttatgatg aaaaatatgg
     9901 atggagtaca gctattagat agaattaaat ctattaatag agatatagag gttatattag
     9961 ttacaggata tggaagtatt gaaaatgcag ttgatgctat gaaaagagga gctttatctt
    10021 attttataaa aagtaatccc attgaaaatt tattagaaga agttgaaaag gtaaaggcaa
    10081 gtaaaatatc tgtttctgta caaaaaaata atctggaatt tactttggaa tctaaaaata
    10141 gagattttaa taatatcata aaaatagcaa aaaaagctgc ttgtaaagat gttaatatat
    10201 taatcttggg ggaatctggt gtaggaaaag atatattagc tagatatata catagcataa
    10261 gtccaagaaa gaatgagata tttgtacctg taaattgttg ttctttttca gaaaatctat
    10321 tggagtctga gctgtttggt catgaaaaag gttcttttac aggagcagta gatagtagaa
    10381 aaggtagatt tgaactttcg aataaaggaa ctctattttt ggatgaaata ggagatatac
    10441 ctctaaatgt tcaggtaaaa cttttgagga cacttgagga caaatctata gagaggatag
    10501 gaagtaataa atctattaaa gttgacttta ggttaatttg tgctatgaat aaagaaccaa
    10561 aagtggaaat atcaaatgga aatataagag aagatttttt ttatagaata agtacaataa
    10621 caataactat accaccacta agaaagagaa gagaagactt aactactctt atagaatttt
    10681 tcttaaataa gtaccaaatt gaacatgata aaaagataca ctctattgat aaggaagtaa
    10741 aggatttttt attaaattac aattatccag gaaatataag agaattaaaa aatataataa
    10801 atagacttgt agttttaagt gaagaaggca atttatcaaa agacaattta aatttaatat
    10861 caaataatgt atacatagat gatgaaataa gtattagacc tttaagagaa ataagaaaag
    10921 agtttgaatg tgagtatatt gaaaaagttt tatctttgtg tggaaataat atttcaaata
    10981 cagctaaaaa attggagatt agtagaagac agttaaccaa taaaatatca gagtataata
    11041 ttaagtgaga aaaaaacttc tcattaagtg agaaaaaaat ttctcaaact gtattaaaag
    11101 ataaacagaa aaaatctgtt tatctttttt tattataatt ttaaaaatta atatttacaa
    11161 taattattgg taaattcaat attggtgtaa attaaaaatg aaaatttcta tgacattaat
    11221 acatagaata tatattttaa ataattggca tagaaaatgc tttatatatt aataaacgta
    11281 ataaatactt ttaattgcga ttaaatatat taagggggat tttcatatga agctagagct
    11341 agggaatata tttataaaaa atgttgaatt tggagaaaag acagaagtaa aagatggaat
    11401 actatatatt agtagcgaag aaatagaaaa gatagtatta gaagatgaaa ggattgtaag
    11461 tgtaaatgtt gaacttgcaa gacctggcga atcaataaga atagctccag ttaaagatgt
    11521 aatagagcct agagttaaaa ttggagatga aagcaaaata tttcctggaa ttataaataa
    11581 agtgaagact gtaggaagtg gaagaaccca tgtactttta ggagcttgtg ttgtaacttg
    11641 tggaaatata gttggatttc aagaaggcgt aattgatatg agtggtccta ctgctaaata
    11701 tacacctttt tcaaaaacaa ataatatatg tattgttata aaagcaaagg atggtataga
    11761 tactcatgat tatgaagaag cagctagaat tgcaggatta aaaataggtg catatgtagg
    11821 tgaggcagga agagaagttg agccagatga agtcattgta tatgagacaa agccattatt
    11881 aaaacaagta aaagaatatc cagacctgcc aacagtagca tatgttcaca tgttacaatc
    11941 acaaggactt ttacatgaca cttattacta tggggtggat gctaaacaga tagtgcctac
    12001 atttatgtat ccaactgaaa taatggatgg agctataata tctggaaact gtgtagcacc
    12061 atgtgataag gttacaacat tccatcattt aaataatcca gttatacatg acttatataa
    12121 aagacatgga aaagatttaa actttatagg agttattttg actaatgaga acgtattctt
    12181 agtcgataaa gagagaagct ctgatatggt agctaaatta atagaatttt taggagttga
    12241 tggagttcta gtgacagaag aaggttatgg gaatccagat actgacctaa tgatgaattg
    12301 tagaaaatgt agtgaagttg gagcaaatgt agttttgata acggatgaat ttcctggaaa
    12361 agatggcaag agtcaatcta tagcagacgc tactaaggaa gcagatgcag ttgtttcttg
    12421 tggtcaagga aatctagtgg ttcatttccc agcaatggaa aaaattatag gaactttaga
    12481 ttatgttgaa atgatgatag gtgggtataa aggatgctta aatgaagacg gaagcatgga
    12541 tgcagagcta caaattataa tagcatcaac tatagcaaat ggctataatc atttgacagc
    12601 aagatattat taaataagta tttgaatata aagttctgtt gatgtaatca ttggtctaac
    12661 tgatgtaata actataaagt gatttggagg attaagagat gagtaaaatc agagtggttc
    12721 attatataaa caatttcttt gctggtatag gtggagagga aaaagcagat attccgccag
    12781 aaaaaagggc tggtgtagta ggacctggaa tagcatttca gagtcaattt aatgatgatg
    12841 cagagataat ctcaactgta atttgcggag atacgtattt tggtgaaaat attgagagtg
    12901 caactcaaga acttttaaaa atgataaaag aagaaagccc tgatttattt attgcaggcc
    12961 cagcatttaa tgcaggtaga tatggagtag cttgtgggac tatttgtaag gcagttgaag
    13021 aagagttaaa tattcctgta attacaggca tgtataaaga aaatccaggt gtagatatgt
    13081 ttaaattaga tttgcatata gtgtctacag gaaattcagc agcaagtctt agaaaggtag
    13141 ttccaataat gacaaaacta ggtcttaaat tggttagagg tgaggaaata ggacctcctg
    13201 aagaggaagg atatataatg agaggaataa gaaaaaattt cttccatgaa cttagagggt
    13261 ctgaaagagc aattgatatg ttggttaaaa agatgaaagg ggaaagcttt gaaactgagt
    13321 atccaatgcc tgaatttgat agagttacac ctatgaaacc tgttaaagat ttatctaaga
    13381 taaaacttgc cctagtaact tctggaggga tagttccaat tgataatcca gacaaaattg
    13441 aatcttcaag tgctactaaa tatggaattt acgatttaac agatatggat tctatgtcaa
    13501 ataaagaatt tacaactata catggggggt atgacagagc atatgtcctt gaaaatccaa
    13561 atttagttgt accactagat gtagttagag agcttgaaaa agaaggtgta attggagaat
    13621 tagtagatta ttttataact actacaggaa caggaacgag tgttggcaat tctaagagat
    13681 ttggagaaga gttttctaag aaattgttag aagatgatgt agatgctgta attttaactt
    13741 ctacatgagg tacttgtact cgttgtggag caacgatggt aaaagagatt gagagagctg
    13801 gtatagcagt tgttcatata tgtacagtag taccaatatc acttacaata ggagctaata
    13861 gaattgttcc agcagtaggg ataccatatc cacttggaaa tcctatgtta ggagaagagg
    13921 aaagtaaaaa aattagaaag aatatagttt taaaagcttt aaatgcacta acagaagata
    13981 tagaagaaca aacagtattt gaataataaa aatacaacac aacactgaaa taaaaaaaga
    14041 tatcaaaaaa taatttttta aaattatttt acgatatctt ttttttatta ggtaaattcg
    14101 aaatttgcat ttttttaaat taaatttata aaagtgataa attagtttat tataatattg
    14161 atacacaaat tcaaattaga tatactatga aaagcgagat tattacattt tggaacaaat
    14221 attcaacata ttagatcata ttaattgaac agaaaaaatt aactttatat aataattaga
    14281 ataatgataa gaaagaaggt gtatatattg atttcaattg ccatttgtga tgatgaatcc
    14341 tatatgctaa atcatttaaa ggaaaatata gaattataca tgaagcagaa gaatttatta
    14401 tataatatag agctttttaa taaagtagaa aatttactat gcaatcatga aaagtttaat
    14461 attgtatttt tagatataca gatggaagag attaacggaa tgcaggcagc tagaaaatta
    14521 aggtcatatg gtaatgagtg ctttataatt ttcgttacag ttttaaatga atttgtattt
    14581 gatgcttttg aagtagatgc tataaattat ctgataaaac ctctatctaa tgagaaacta
    14641 tataaaactt tggataaaat agttacaaaa cttgatgata atgaaagtaa ttatatcaca
    14701 atacaaaaag gttattcata ttggaaatta aaacttgctg atattttata ttgtgaagtt
    14761 ataaaaagaa agatttatgt tcatttaaag aaagagactg tagattatta tgagactatt
    14821 gaaaatttag agagaagttt accaacaaat ttttttaggt gtcatcgtag ttatataatc
    14881 aatttacaat atgtatatgg atatgaaaat ggtaatgcag ttatggaaag tggagataga
    14941 atacctgttt ctagactacg tcaacaaata ttttcaaaag ttatgcttga gttcatgaaa
    15001 aataggagga tataagatga ttaatttatt ttcttccgat ttatttttaa agtacagttt
    15061 tttcttctct tttattgtat atagaatttt aatggtaata gtgcaatact attttctaat
    15121 caattttaca aattttaatt caaaattttg gtgttttatt ttatattcga taatttcaat
    15181 ttttttaatc attataaata catattttaa tttatataat ttattttctt cacagattct
    15241 attttttctt atgacttttt tatttgtaaa gtacataata aaacaatcta ctgttttatc
    15301 tatagtatca actttgttag tatatttaat tgaacaatta gtatatggat ttacagctcc
    15361 attaaattac ttgatattta ctagtgatat aaatacagat atttataaat ttgatataaa
    15421 tatgggacta tcttctttaa ttactattat aatagctgga tttatatatt ggtatagttc
    15481 taaaaagttt aatattaagg tcatgaactt tgataaatat atagcaattt tgatgattcc
    15541 attgttgctt ataatattat ttatgcaatc atttgaatat agttcaaata taaatataga
    15601 tacaagtctt gggattgtaa agctgctatt aaatacttct ataactgaag aaattcaagc
    15661 ttttcttttt tctataattg gaactatatg tgtatttttt tctctattta cctttaagaa
    15721 actaattcaa gctctcgaag atgacaagga gagggcaatt atgaatcaac agattcatgc
    15781 tcaaaaaaac tatattgaag aagcgaaatc acgattatca caaactatat cctttagaca
    15841 tgattttaat aatcatttag ctattgtcaa tggattgttg aaaaaagacc aaattttaaa
    15901 agcacaagat tatttaaata agcttgaaaa agtaacaagt gacctatcat tttcttgtaa
    15961 tacacagaat attgttattg atgctctttt caacaataaa ttaagtatag caaaacaatt
    16021 aggcatagag gtagattgtg aagtaattat accccaaagc actaacatga acgattttga
    16081 cctatgtata gtgtttgcta atgctattga caatgcaatt aaagcttgta gagttgtaga
    16141 taaacaaaat aggtatttaa agctttccac taagttagag ggtggatttt ttatgataga
    16201 aataaaaaat agctataatc caataaataa ttatacaagt ggctcaggta taggtctttt
    16261 aaatataaaa gaaattgcta aaaaatatca aggggctata agtatagata agacatcaga
    16321 ctattttcaa ataaatgtat tgctagttat ttcattacat tgcgctagta tttgagaaca
    16381 atttcattga aaatggtatt tgtaagctat attatgtact tgaatagaga aagtttgata
    16441 tatagaaatg aggaggttac taaaatgaaa gttggaaata taaatggtca agcaccaatt
    16501 acaaatggtt tagatagctg tcaaagtgag gtacaaggct tagaacaaaa gaaaaaatca
    16561 ttacaggatg aaatagagaa agtaagaagt gataaaagta ttgatggtga aacagccaaa
    16621 ttaaaaatag aagctttaaa aaaagaaatg gaagagattg aaaataaaat tcaagagtta
    16681 aaaggaaata aagcaaaaaa aacaaataaa gaagaaaata atgtttcaga agtaaataat
    16741 aatctattag agagaaaaaa agaaccttct aataatgaaa atgtaaaaca attatatgat
    16801 gtttttgaaa gaagcaatgt agacgaacaa tgggataata cttatcgtgt tctccaagat
    16861 gataaaaact taaaagtaaa atttaataga cctatatagt ttatttgtaa aaatagttaa
    16921 tattttaatt atcaccactt tttagaagaa tacacacttt attttaaaga cccacatatt
    16981 tatttacagt tagcacattt atatgtggtt ttataactat tatttacaag tatatattga
    17041 taacttttac atagtatata atgtttaaat acttattcaa atttatctat acataaacaa
    17101 aaaaagagat tttaaaatct ctttttagat tgttgactta tggctttttg atttaataaa
    17161 atcaagaagc cgtttgaatt ttatatgttg gttgataccc ttctgatatt gaaacaccat
    17221 ttttgtttcc aaaatcgttt aaaattaaga acttattatg aatgactatt ccattatgaa
    17281 caataagcaa atgacctgaa tcttcaatca atgttttaac agtagcatta aatgcttgct
    17341 tatacagttc tatcgtttgt ttgaactgcc cattaaagta aatatgagtc aataacataa
    17401 ctatatctac acaaattaat ataaattttt tatactgtac attatctaac tgttgattat
    17461 ctgtattaca acatgattga atttaaaaag ccatcaacac atagaatgtt gatggcctga
    17521 aaatagattt aaaatctctt ttttattata tattaattat tttttaaatt tatatttttt
    17581 gcatcagatt ttctaatagc taagtcaaac cctctaaatc taatcactat agggtcatta
    17641 agaggagcta tctttttcac ttcaacttca gtgccatcta ttagtcctaa ggctagaagt
    17701 ctcttcataa gtttttcatt tccagctata ttgtctataa ttcccttttg tccaagtttt
    17761 aagttgtaaa cagtcattat taacacctcc taatgaaaat cattatcaaa ctttatattt
    17821 ataatatata acaaaaagag caaattgtca atatataaca aaaaataaac atactaagtt
    17881 agtatgttta taaaaaatag tatacatggg atactaatag catctagcaa aaaaccactt
    17941 actaatggta caaccaaaaa ggcttttgga gaatcaccat atttttgtgt caaagaactc
    18001 atatttgcca aagcgttcgg agtagctcct attccatgtc caatcaaacc gcttattatg
    18061 atggcagaat caaaatcttt tccaagtact ctaaagcaaa taaatattga aaatagaatt
    18121 ataaagatta cttgaaaagt tactattaag agaataggag gtaataatgt agataattca
    18181 aataaatcta tattcataag tgataaagtt aaaaaaattc ccaatgaaag ttcttttaaa
    18241 aaatctataa gtttaaaatt taattctagt atatttatct tatcatttaa atttctaaaa
    18301 ataacagccg aaaacataca agttacaaca gagggtaata gtatattaaa cgttatatag
    18361 aagcatctac ttataatttc tcctgtattt atacaaataa atatgagaag aacctgttcc
    18421 aaaaacacag atggtgttat tgtttcggag aagttattat tttttatcct attcttattt
    18481 ctaaaaaatt tacttccaaa tctctttaga tttctattat atctaggaat cgacaagtct
    18541 atatttgatg atggttttaa tttatattta tttattagaa atttagctac aggacatccc
    18601 aatataccac ccataataag tcctattgtt gcagcagata atccaacccc aactgctcct
    18661 tctataccga gattttctat tgtaagtcca aatgcagcag aataaccatg accaccttcc
    18721 atagatacat ttccacacat aagccctaaa agaggttcaa gttttataat ctttgatatt
    18781 gtaatagcta atatattttg acaaaatgcc aatactccac acaaaatcca atatctaaat
    18841 aatacactac cccctttctt tataagattt atgcttattt cgagtcctat agttgtaaaa
    18901 aagaaggtta taaaatttgg cattaaattt ccacttatag atatatctgc tatattaaaa
    18961 tatttaaaaa ataaatttag aaaacaaaat aatagacctc ctattactgg tgcaggtata
    19021 cagaaatcgt ttaaaaagtt aactttattc ttcaataaat taccaattaa aaagaagaat
    19081 atagatagtg tcaaggtttg cactatatca aaacttaagg taatcaaaat aagtcctcct
    19141 ttgtaattca aagttttaat tatataactt aaattcttat gtattgaaat tttattatca
    19201 aatgtatatt aataaatgta tattaaaaag aatcagtaga tataattaga tagtcataaa
    19261 ttaaattaaa atattttaat gagatatgac ttttttagaa ttaaaaaccg tagtatattg
    19321 atataataga aataagactt aaaaatctgt tgatagtatt atttataagt acagtaggag
    19381 gataaaatgt ttgaaaattt acctaagata gatttacatt gccacttaga tggaagtgtt
    19441 agagtagaaa ccatgcttga tatagctatt aaagagaaaa tagatttacc ttcaaataat
    19501 atggatgaaa taaaaaaatt agcaaaggtt tcttttaatt gtacttcact agatgaatat
    19561 ttagaaaaat ttgatttacc cttaaaagta atgcaatcta aggaaaattt aaaaagaatt
    19621 acttttgaac ttttggaaga tgcaagtaga gaaaatgtaa aatatattga aattagattt
    19681 gcaccattac ttcacaccca aaaaggtatg agtgttaaaa atataattga gggtataatt
    19741 gaaggaatta gagaagctga aagtatttat gatataaaag gcaatttaat attaggttgt
    19801 atgagaacaa tgacctcaaa agaggcatta ttagtaattg aagaagggaa atcatttgta
    19861 aataaaggtg ttgtagcagt agacttgtgt ggtccagaga aagagggatt ttgtaaggaa
    19921 tataaagatg tttttaaatt agcaagagaa tatggatata aagttacaat acatgcagga
    19981 gaagctgcaa gtggagaaaa tgtattggat gctataaata ttttaaaggc ggataggatt
    20041 ggtcatgggg taaaaattaa agaccatgaa aaggcgtata atttagttaa agataaaaaa
    20101 atattgcttg aattatgtcc aactagtaat gtacaaacta agacagtaga ttcttatgag
    20161 gtacatccat tttatacttt ttataaagat aatttacatg taagtataaa tactgataat
    20221 agaactgtat cagatataaa tttaagttca gaactaaatg ttatatttga tacttttaaa
    20281 ctaggattag aagactataa aataatttat agaaatgcag tagaagctag ttttgcagat
    20341 aaagaaacta aagagtattt gaatagttta atttaataga taaaaatatt taaactttaa
    20401 attaagctat taaattgaca aaaatacatt gtattaggag aaaaaatgtg aaagattatg
    20461 aagatgttgt ttatattaaa ctagtcaaaa aagaacttga agaatggaag aaaaatatat
    20521 taaagaaacc ttctatattt gacaaagcat caaagggtat acagaataaa tttaatggag
    20581 tattgccaga taaatatcat gaaataatta cttcttctat aaaagtaatg acaaaaaccg
    20641 tactgtttgg atataaatat gctacaaagc caccacttag aaatatgtca ttagaagaaa
    20701 gagaaaaatt agttttagaa aaagctaaaa cttacaagac cacagcaatg attgaaggag
    20761 caggaacagg ggcaggagga atcctcttag gtttatctga ttttcctttg cttttaggta
    20821 ttaaagtgaa atttttatat gatgtagctt caatatatgg atatgatgtt aaagattata
    20881 gagaaaggat ttatatatta aatatattac agttggcttt ttctagtcaa cagcatataa
    20941 acaaaacttt taaattgatg aataactggg atgaatatgt aaatagtcta ccagaagaca
    21001 tcaatgattt tgattggaga acatttcaac aagagtatag ggattattta gatttagcta
    21061 agtttttaca actaatgcct ggaataggag catttgtagg attttatgtg aatggaaaat
    21121 taattgataa attatgtgaa actgctgtgt ctgcttatag attgaggatt cgagagctta
    21181 agttataaga gaaattataa cataaaaaat tagttataca atatatctat atgtatctaa
    21241 ttttgcattg ataaaaacaa ttatggataa aaggtaaagc atgatagaat ctaatgtaga
    21301 ggtttgtatt ttaatggaaa aatttagttt ttattgctta agatacaaat tgaattacga
    21361 agagaagtaa ttgggaggaa tattttcatg acaaatttgc caaaacaatt tgatagcttc
    21421 aatgaagcta gacaaaaagg atttataaat gcaaaggaac ttaaggaaag tgggaaaaag
    21481 atggttggag ttttctgtac atttactcca gttgaaattc caatggcagc tggagcaact
    21541 gtagtaggtg tatgcggcgt tagtgaagag ccaatacctg atgcagaaag ggtgcttcca
    21601 agaaatcttt gtcctttaat taaatcaagc tatggacatg caataacaga tacctgtcct
    21661 tatttctatt tttctgattt gttgatagga gaaaccactt gtgatggtaa gaaaaaaatg
    21721 tatgaagaac ttgcaaaagt taagccaaca tatgtaatgc atttgccaaa tacttcaaaa
    21781 ggagaatttg cctataaact ttggaaagat gaaatgataa gattaaaaga agaggttgag
    21841 aaatctttag gtgtaactat aactgaagaa gatataagaa ctgctataaa agataaaaat
    21901 gaagaaagag aacttttaaa agagttttat gcacttggta aattacaacc atctgcactt
    21961 acaggccttg agttacataa cgttttatat caagcaggtt ttaaatttga ccgtgcagaa
    22021 cttaaacatt ctttaagaaa agttatagat gatatgaaag aaagatatga aaaaggtgaa
    22081 tgtcctgttc aaaaagataa gccaagaata ttgataacag gttctcctat tggtggaata
    22141 agcgaaaaaa tagttaaaac tttagaagat gcaggggcat cagtcgttgc atatgaactt
    22201 tgtggagcta ttagatcaaa cgatttattg gttgatgaag aaatagaaga tgtatatgat
    22261 gctttaacac aaaaatatat aaatataggt tgttcttgta tgatgaataa tgacaatcgt
    22321 attgaattat tagatagaat aatagatgaa tataatgtag atgctgtaat agatgtagtt
    22381 ttacaagcat gtcatacatt taatatagaa agttatagaa taagagaatt tgttacaaaa
    22441 gaaaaaaata agccatttat gtctcttgaa actgattatt caaaatcaga tactgaacaa
    22501 ttaagaacta gatttgaagc gtttgtagag atgttgtagg aataaataaa agtaggagga
    22561 actatgttta gtataggagt tgactcaggg tcagtggcaa caaagggtgt actatttgat
    22621 ggtgaaaaaa ttattaagaa aataattata ccaactggat ggagccctaa aagtacatct
    22681 aaacaggtct atgagttgct aagtagtgaa attgacaaaa aagatataaa aaaggtggta
    22741 ggtacaggtt atggaagggg agtaatggat tttgctgata agaaagtaac agaaataact
    22801 tgtcatacta gaggtattta ctttttaaac aagaacataa ggactatttt agatgtaggt
    22861 ggtcaagata gtaaagttat aaatttagat agagatggaa atgtatttaa ctttataatg
    22921 aatgataagt gtgctgcagg aactggtcga tttttagaga ttacttctaa ccttttagga
    22981 agcgatattg aaagtataga tacattagct aaaggatatg aaccagttaa tatatctagt
    23041 atgtgtacag tttttgctga gtctgaaatt gtaagtcttc tagctcaaaa catttctact
    23101 ggagaggtag cagcagggat acttaaatct attgcaaata aatccacctc aatgttagca
    23161 agaggtgagg taatagatga agttgcattt actggagggc ttgcaaaaag taaagaacta
    23221 gtaaaaatga tagaagaaat actgggtaaa aaaattttta ttgcagaaga tactcaaata
    23281 ataggagctt taggagctgc tgtaatagga tttagataaa aacatataaa aagaaaatga
    23341 gaagctgaat ttattataaa taaattcagt tttttatttt attttattta tttataaagg
    23401 aaaactttgg tataattgat ttaaatgatt gtatatttgt aaatacttga tatttagtca
    23461 tatataacat tataatttta aaaattagct caaaataagg gggagaaagc gtgaaaaaat
    23521 ttacttcaaa aaaagtaaca aggtttgttg cgctgttttt aatatctatt ttagtaatgt
    23581 caacaattcc tgtatcagct gacaatagca atacattgaa tcagataaaa aaagcagaat
    23641 acagtgagac gtataaacaa tatctaaatg atgctaaaaa tggacgtaca gaaaaatata
    23701 atgggattat accaagtcca tatcagttgg aaggtactca aatccaaagt aaaggaagaa
    23761 cagctccaac atcttatgac cctagaaaat taggtcttat gacatcaata aaaaatcaag
    23821 aggatttagg aatatgttgg gattttgctg caatggcaac cttagaaagt ttcttaaagt
    23881 taaataatta tggagactat gatttatcag aagaacactt aagatggtgg gcttcagatg
    23941 gtgaatatgg atggtctgta gatgatatga atggtgcttt aaattatgaa gctatgggat
    24001 atttaacatc ttggtcaggt cctaaattag aaaaagatat cccatacaat ggtagagtat
    24061 ctaaagcaca aggagcaaaa aaaccaacta atatgaatac agcacctact gtttttaatg
    24121 tgacagatgc tgtgtgtgta agcaatgaca taaattctac aaaaaatgct atattacagt
    24181 atggagctgt aacatctggt tattatgaag atattaaata tcaaagtgat gaccaaaatt
    24241 catattattg tccaacaaaa agttttaata caaatcatgc tatttcaatt gtaggttggg
    24301 atgataacta ttctaaagat aattttaatg caaatattag accatcaaaa aatggtgctt
    24361 ggttaataaa aaatagctgg ggagattata attctgaggg tggatatttc tggatatctt
    24421 atgaagacaa gacacttatg tctgatatag ataatttttc aatcaaaggt ggtaaaaaac
    24481 caaatgatga tgagaagatg tatcaacatg actatgcaag tattgtacct ttaatttcaa
    24541 aaaagataac agctgcaaat gtatttgatt tcaatagagg cgatgaaact ctaaaatctg
    24601 taatgttctt aactgaaagt ataggagcta agtatgaagt atattatgct ccagtagtaa
    24661 atggtatacc tcaagaaaac aatatgaaaa aattaaaaga aggtactgcc caatattcag
    24721 gatacataac agttccaatt gattcttttg atataccaga aggaaagggt gcaattgtag
    24781 tcagcataga ggggaaaaat ggagagtcaa ctataggttc tgagtcaaat gtaccaggat
    24841 atgatacatt taaagcaaaa gctaatttag gtgaaagtta tatcatagat aatactggaa
    24901 aattctttga tataaataga gatagtaact tctatccgtg taattttact ataaaagctg
    24961 ttacagaaaa gtcttctggt gagagtatcc caaatgagtc tttaattggt agtgatagat
    25021 atgaaactgc tatcaaagtt agtcaaaatg gtttcaattc ttcagaaaat gtagttttag
    25081 taaatggcag ttcaatagta gatgcattgg cagcaactcc ttttacatct gctataaatt
    25141 caccaatatt attgacacaa aaagaagctt taaattcaaa aactaaagct gagatacaga
    25201 gattaggtgc taagaaagta tacctaatag gtggagaaaa ttctataagt aaagaaattg
    25261 aacaacaatt aaaaagctta aatatatcga tagagagaat atcaggaagt gatagataca
    25321 agacaagttt attattagct caaaagttaa atggtataaa aaatgtatct caaatagcag
    25381 ttgtaaatgg agtgaaagga ttagctgatg caattagtgt aggcgctgca gcagcagaaa
    25441 ataacatacc aatcatactt gcaaatgaaa agagcgaatt gcaaggagct gatgaatttc
    25501 taaattcatt aaatatagag aaatcttata ttattggagg aacagcatcc ctatcaaaca
    25561 atctagaaag taagttaaaa aatccaacta gattgtctgg tagtagtaga gacgaaacta
    25621 attcaaagat aatagataat ttttataaaa aagatacttt aaaaaatgca tttgtagtta
    25681 aaaatggaat caagaatcaa aatgatttaa tagatggatt ggcagttggt cctttaggag
    25741 caaaaactgg gtcaccagta attttagttg gtgataaatt agctgatagc caaaaagaag
    25801 ttcttaaaaa taagacttta gaaaaagtaa ctcaagttgg tggaggagca aataaaaatg
    25861 catttaaaga gttagtaaaa ttaaaaagtg ctaaatagag tacttaagta ttaacatata
    25921 ttagtgtgta ttagtaactt atcttaataa aagctgaaaa taattacttc aaattattga
    25981 taaataagat aatcaatgat tgtaaagttt tgttttcagc tttaaattta tgtagtataa
    26041 gtttaa
//
LOCUS       DACXMZ010000030        25904 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.030, whole genome shotgun sequence.
ACCESSION   DACXMZ010000030 DACXMZ010000000
VERSION     DACXMZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.030"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(93..1100)
                     /locus_tag="JD016_04935"
     CDS             complement(93..1100)
                     /locus_tag="JD016_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HAU4998976.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            1328..2212
                     /locus_tag="JD016_04940"
     CDS             1328..2212
                     /locus_tag="JD016_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAU4998977.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            2414..3964
                     /locus_tag="JD016_04945"
     CDS             2414..3964
                     /locus_tag="JD016_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAU4998978.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            3993..4640
                     /locus_tag="JD016_04950"
     CDS             3993..4640
                     /locus_tag="JD016_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAU4998979.1"
                     /translation="MRKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDINYLNISEL
                     SGLSKADIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(4782..5042)
                     /locus_tag="JD016_04955"
     CDS             complement(4782..5042)
                     /locus_tag="JD016_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
                     /protein_id="HAU4998980.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            5259..6542
                     /gene="hflX"
                     /locus_tag="JD016_04960"
     CDS             5259..6542
                     /gene="hflX"
                     /locus_tag="JD016_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAU4998981.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            6550..7227
                     /locus_tag="JD016_04965"
     CDS             6550..7227
                     /locus_tag="JD016_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HAU4998982.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            7313..7756
                     /locus_tag="JD016_04970"
     CDS             7313..7756
                     /locus_tag="JD016_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAU4998983.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            8061..8705
                     /locus_tag="JD016_04975"
     CDS             8061..8705
                     /locus_tag="JD016_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAU4998984.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVVVVVTRYFG
                     GTKLGAGGLVRAYTKGTKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(8791..9168)
                     /locus_tag="JD016_04980"
     CDS             complement(8791..9168)
                     /locus_tag="JD016_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAU4998985.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(9168..9554)
                     /locus_tag="JD016_04985"
     CDS             complement(9168..9554)
                     /locus_tag="JD016_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAU4998986.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            9733..10488
                     /gene="nadE"
                     /locus_tag="JD016_04990"
     CDS             9733..10488
                     /gene="nadE"
                     /locus_tag="JD016_04990"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HAU4998987.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            10571..11290
                     /locus_tag="JD016_04995"
     CDS             10571..11290
                     /locus_tag="JD016_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAU4998988.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(11457..12461)
                     /locus_tag="JD016_05000"
     CDS             complement(11457..12461)
                     /locus_tag="JD016_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAU4998989.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            12727..13623
                     /locus_tag="JD016_05005"
     CDS             12727..13623
                     /locus_tag="JD016_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HAU4998990.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            13744..15006
                     /locus_tag="JD016_05010"
     CDS             13744..15006
                     /locus_tag="JD016_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4998991.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            15082..16275
                     /locus_tag="JD016_05015"
     CDS             15082..16275
                     /locus_tag="JD016_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HAU4998992.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            16916..17698
                     /locus_tag="JD016_05020"
     CDS             16916..17698
                     /locus_tag="JD016_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAU4998993.1"
                     /translation="MENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQVIDVV
                     SENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEKTVIA
                     AVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTGIAKE
                     LIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVINKGI
                     DMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            17793..19133
                     /locus_tag="JD016_05025"
     CDS             17793..19133
                     /locus_tag="JD016_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HAU4998994.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSTAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            19264..20502
                     /locus_tag="JD016_05030"
     CDS             19264..20502
                     /locus_tag="JD016_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HAU4998995.1"
                     /translation="MKVLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFG
                     DPGRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSS
                     YRTGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDI
                     AEKDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSL
                     LASTQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIK
                     EEKFASSVESKKNATELISILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQ
                     AIANNYIYEYTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSD
                     IEALIESGIVTMNKEACEIC"
     gene            20542..21684
                     /locus_tag="JD016_05035"
     CDS             20542..21684
                     /locus_tag="JD016_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAU4998996.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            21695..22489
                     /locus_tag="JD016_05040"
     CDS             21695..22489
                     /locus_tag="JD016_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAU4998997.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            22500..23522
                     /locus_tag="JD016_05045"
     CDS             22500..23522
                     /locus_tag="JD016_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAU4998998.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            23621..24997
                     /locus_tag="JD016_05050"
     CDS             23621..24997
                     /locus_tag="JD016_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAU4998999.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(25329..25673)
                     /locus_tag="JD016_05055"
     CDS             complement(25329..25673)
                     /locus_tag="JD016_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999000.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT         9783 a         3035 c         4839 g         8247 t
ORIGIN      
        1 atatttggtc tgtaggttaa ataaaagtac ctttagacca aatatattat taataactta
       61 ataacatctt aaaacttatt tatttatatt atttacaaca tccatttaat aaatacacca
      121 taacctacag ctgggtcgtt ttcaattgca tgtgacactg ctccgattat tttattatct
      181 tgaactattg gagtaccact cataccttgt acaataccac cagtttgagc taaaagcttt
      241 ttatctgtaa ttttaatttt aaaactttta ctttctggat gtttttggtt ttctatatct
      301 ataatttcaa tattatattt tttacactta ttaaagttat cttgtaaaat tatttgagct
      361 tttcctaatt taacttcatc taaagatgct actttatatt tttttaactt ggaaatatca
      421 aagttattaa tttgacctct tattccaaaa tcagtatttt ctgtaaagct accaataata
      481 ttttctcgtt ttgcatttat acatccaaca ctacctttat aagatttttc tatattaagg
      541 tgcttagttg tagaaattga acctgtttta ataggtattt ttcttgcatt accaacactt
      601 attggatgac ctacagctcc aaactctgat gtatctggat tgatatacgt taaagtagca
      661 aatccagaaa gtaaattgtt aaaactaatt ttttctaata tgtgtttggt agttttcatt
      721 gttataagtt gtccacctct atttaataaa atagatactt gtttttcttc tggtaaagca
      781 tttaaggtat tggcaaagtc actatatcca tcaacttcag cattatttac ctttactata
      841 gcatcaccta atctaaatgg agatgcttct ccaggatttc taactatgat attttttaat
      901 tctgcgtcaa tttgaagtac gtttccaatt ggtattaaat attcaggttc atttttttgt
      961 aaattttgtg cataaattaa attatttgag aaaaaatata ataaaaataa aaatgttaaa
     1021 acaattgtaa attttgactt agctaagttg gtttttttac ttaaagtatt atttttaaaa
     1081 tgaaaatttt taagttgcat aaaacacctc tttcttatta aattatttta ataatagaat
     1141 tgaagttttc ttgctgcttc tatattatgt tgtgcaagaa tcatgttttg atgcttggaa
     1201 aactagctca ataacacctt agttttcata aaaataaata aaataaatag tgtatatgat
     1261 aaaataaata catattcata taaataaaaa taaacaataa ctgtctagca taagaaagga
     1321 gcaaaaaatg aaaaaatata gattgttagt tacttgtata attgccatgg gaatcataat
     1381 tataggaaca tcaaatccag ttagcaattt ttacaagact acaaatttga ttagttcagg
     1441 aaatatcaca aatgaaaaag cagataaaga tgaaaattca aacaaaaata ataaagataa
     1501 aaatgaagac aaatctaatt ctaaaagtaa taaagataag tcatcagagg acaaatcgtc
     1561 agataataaa aatgaaattc aaaaaaataa aaaactttta atttgtatag acccagggca
     1621 tcaaggaaaa ggagatagca atttagaacc agtagctcca ggttcttcct ctaaaaaagc
     1681 gagagtatct tcaggaacag agggtattgc tacaaaaaaa ccagaatatg ttttaaactt
     1741 agaagcatct cttgttttaa agagtatatt agaatctaaa ggttataatg taataatgac
     1801 tagagagaca catgatgtaa atataagtaa ttcagaaaga gcaatacttg ctaatgacaa
     1861 aaaagcagat atggttgtaa gaatacatgc agatagttta aataattctt caaaaactgg
     1921 agcatctata ttgattcctg aaaaagatgg aaaatataca gctcctatat atgaagaaag
     1981 taataaatgt gctgaattta tcaaacagaa tatggaacaa tcaggtatac aaatcaatgg
     2041 aatcgttcaa agaggtgatt tgactggatt caactggtca aaagtaccag ctgtattagt
     2101 agaaatggga tttatgagta actataatga agaccaaatg atgtcaaatc cagactacca
     2161 aagaaaaatg atgcagtgta tagctgatgg attggatgcg tattttaaat aagatatgaa
     2221 tagagaaaaa gttaggttgg tattatttgc ctaattttta tctctatttt ttattgtaca
     2281 taacacaaag aagaagttta ttttaggtta aattgtcgta aatataacta aaaatattgg
     2341 gaatacttga tttaagttta aaaagaatat attatagtat agaatgtgta aaattttttt
     2401 gagaggtgtt gaaatgctag tagtagaaaa tgtgagtcat ggatttggtg cgagaacaat
     2461 attagaaaat gtatccttta gacttagaaa aggtgaacat attgcactag ttggtgctaa
     2521 tggagaagga aaatcgagtt ttttaaatat aataacaaag aagcttatgc cagatgctgg
     2581 aaatataaaa tggtcttcaa gagctacagt aggttactta gaccaacata cggtattaag
     2641 caagggaaaa actataagag aagtgcttag agaggctttt aagcatatgt ttgacttaga
     2701 gcaagaaatg attgctatgt atgacaaaat gggagaagct agtgatgatg aaatgagtaa
     2761 gcttcttgaa gaaacagcag agatacaaac tatacttgaa aatagtggtt tttatatgat
     2821 agatgcaaag atacaagaag ttgcaaatgg tcttggtctt ggagaaattg gtcttgataa
     2881 agatgtaact gacttaagtg gagggcaaag aacaaaagta cttcttacta aacttctact
     2941 tgaaaatcca actattctta tattggatga gcctacaaac tatttagacg aagagcatat
     3001 aacatggctt actaagtacc ttcaagagta tgagaatagt tttgtcttag tttctcatga
     3061 tattgaattt ataaataata catgtaatgt aatatatcat atggaaaatg gagagttaaa
     3121 tagatataaa ggaaattatg atgaatttgt aagacttaat gatataaaga aacgtcaaga
     3181 agaacaagct tatgataaac aagttgaaga aagaaaaaga ctagaggatt ttgtagctag
     3241 aaataaagct agagtagcaa ctagaggaat ggctaacagt agacaaaagc aacttgataa
     3301 gatggaaata ttagaaagac caaaagaaaa aataaaacct acttttgctt ttaaagatgc
     3361 tagagcagct agtaaaataa tttttgaaac agaaaattta gttttagggt atgatgaagc
     3421 attgacaaaa ccacttaatt tccatttaga aagaggaaaa aagattgcct taaaaggaat
     3481 gaatggtatt ggaaaatcta ctctattaaa aactttactt ggaataataa aaccatttga
     3541 aggaaatgtt aaattaggtg actatttgga agttggatat tttgaacaag agagttcaag
     3601 agaaaatagt aatacaccta tggatgaagt ttggagtgaa tttccagggc ttacaaactt
     3661 tgaagttaga caggctcttg caaaatgtgg acttacaaat gaacatataa cgagtcaaat
     3721 gagagtatta agtggtggag aagctgctaa ggttagactt tgcaaagtaa tgttaaaaaa
     3781 tataaatttc ttagtattag atgagcctac aaatcattta gatgttgagg caaaagatga
     3841 attgaagaag gctatcaaag agtttaaggg aacagttctt ttagtatgtc atgagccaga
     3901 attttattca gaaattgttg atgatgtatg gaatatagaa gattttacaa ctaaaatcgt
     3961 gtagtgattt aacttattgc tggggggatt tcatgagaaa gtttctaaat gtactttttt
     4021 caatttgtat tagtattgtt ataatagtag gtgttattaa ttttactgtt ggatttaaac
     4081 aactttatta ttttgacatt aattacttaa atatatcaga gttatctggt ttatctaaag
     4141 ctgatataaa attgaactac gattatctaa ttgattataa cttaaataag aatgtgtctg
     4201 agttcaaatt accaacatta aaatcttctc cacaagggaa gatacatttt gaagaagtca
     4261 ggaatatatt tcaaaatata aataaattag ctaaattatt attggtagtt tctttagtag
     4321 gaattatatt gagtgtaaaa aataaaaata taaaaatctt aaaaactaca tcaataacac
     4381 ttataattat gccattatta ttaacagttc caattttatt aaattttgaa aagtcattta
     4441 ttatttttca taaattatta tttagaaatg attattggat atttaatccc gatttagacc
     4501 cagttataaa tatattgcca gaggaatttt tttttcactc tggaatgatg attttaatat
     4561 taattttgtt agtaagtata gtactgtttg taatgtataa attatgtaaa tctttatcaa
     4621 taaaaatatt tcaatgttaa atagagggat atttttattt ccaaaataag ataataaaaa
     4681 aggattagat taaaagttat acaataaaaa tagctgtgca ataatattta gactatgaaa
     4741 gtagttaaaa aatatttatg cacagctatt tataatagaa tttacttttt actagtgatt
     4801 ttaactaaat cttgaaattt actcataatg taattgtaat tatctttttt atgaggtaat
     4861 atacaactac tgcaatcttt gataccttta tctgtatact taaagtttcc accacagttt
     4921 tctcctaaag catacaaagg acaatagcaa aatagacagt taaactcttc tggtttatta
     4981 gttttatgac aagggaaaaa ttcgcaatct ttgtgattaa aaaacttata attttcgccc
     5041 atattttcct cctaattaaa taaaatttaa attttttata ctatataaga ttttggtgat
     5101 aatataatta tactaaataa ttttatgaat agtcgataat atagtattat taatcatagt
     5161 attaatggta taattagtca acagaaaaat aatgtaaaaa taatgtaagt gtattttgta
     5221 attagaatat gttttataac aaaaaatgag gtgattaagt gaaagaaatt caagaaaaag
     5281 cacttcttgt aggtctgaat ttgactacaa tagtgaaaaa aaatgatgat atagacacta
     5341 atgaatctat ggaagaatta aaagaactaa caaaagctgc tggggcagaa gttgtaggta
     5401 gcttaattca aaataaacac tctgtagatg cagcttatta tataggaaaa ggtaaagtag
     5461 aagaaataag agcctatagt gactctttag atgctacttt agtaattttt aatgatgaat
     5521 tatctggtgc tcaaatcaga aatatagaga atgttgttgg aagaaaagta atagatagaa
     5581 ctactttaat tttagatata tttgctcaaa gggcacttag caaagaaggt aaattacaag
     5641 ttgaattagc acaattaaaa tatagattgc cacgacttta tggaatgggt ggtgagatga
     5701 gtagaacagg tgcagggatt ggtactagag gtcctggaga acaaaaacta gaaatagata
     5761 agagacatat acttaataaa gcagcagata taagacgtga acttaaagaa gtaaaaaaga
     5821 atagagagac acagagagtt aagcgtttaa agtcaaatat accgattgta gcacttgttg
     5881 ggtatacaaa tgcaggaaaa tctaccctgt tgaacgaatt aataaagaca cacaaggatt
     5941 atgaacaaga aaaagaagtt tttgtaaaag atatgctttt tgcaacttta gatgtaactt
     6001 tgagaaaggc tcttttgccg aataaaaaag aatttttagt agtagatact gttggatttg
     6061 taagtaaact tccccatgat ttagtagaag cgtttaaggc aacattggaa gaagtacagt
     6121 atgcagattt aatcttacat gtaattgatg caacaaatac tagttatgaa ttgcaaaaaa
     6181 gtacgactga aggtgtatta aaagaacttg gtgtaaatga taaaaaacat atacttgtat
     6241 ataataaagt ggacaagcta gaattggata tatatcctaa aagtcaagaa gatattgtgt
     6301 atatttctgc aaaacaaggc ataaatatgg ataagttatt aaatatgata gaaattgctt
     6361 taatggaaaa tacttattca gtaagtttga tgttacctta tgagagaggt gatatattta
     6421 gtagaataaa agataaatat aatgtggaga attttgagta tggggaaagt ggtataacgc
     6481 tagatgtaga tttagatgaa gaagatttta atatctatag agagtacata ctagaaaaat
     6541 aggttgaata tggatataaa ctgggaaaat atagataatc ttgaagatta ttttataacc
     6601 tatcttcttt ataaagaatc taaaacagta tctcaaatta gtaaaattag aaatatttca
     6661 agtactgaag caagtgagca acttatacaa gcaaagttaa aaataaagga aatgcaaaaa
     6721 gatgattttg aggcatctaa ggatattttg gataaatttc tagaactaga taaaattaag
     6781 agacttgatt ttatggatag cttagatgac gaaaaaatgg tatactttaa gagaaaggtt
     6841 ttcaaaagaa tccttgtaga aaaaaatgct gaagatttaa ttgttttaat ttgggcgaca
     6901 ggtgagctaa aagatgatag gtttttgaaa ttgcttcatc agctcacaaa ccacagacac
     6961 tcagatatta gaagaataac ttattcagca atcagaaaga ttgaatcacc aagtagtaga
     7021 gaagtactgc aaaaaggtct ctacgacaaa aatgcacaga ctaggcagta ctgtgcaaaa
     7081 gctctttcaa aattgggaga tgaaaacagt ttaaagattt tacagcagtt gaaagacaag
     7141 aataagaatt ttgaaaaaga atatgtgctt agagcttatg acgaagccat taagtcattg
     7201 gaaaatgcaa aaaatttgaa aaagtaattt tttattaaag caatttgttt acttaatgat
     7261 gtatttctaa agtttaacaa ggtaataaat aaattaatgg gaggggtttc aaatgatagt
     7321 aagacgttgt gcaggtggag tagtgtttta tgctaataaa gtacttattg taaaaaatga
     7381 taggggtgaa tggacacttc caaaaggaaa gatactagga gggggacttc catatgagag
     7441 tgctgttcaa agagttaaag tagaaactgg catagatgct aaaatgatag atgtggctgg
     7501 cgatactatg tatgaatttt tctcaagaag tagacaacaa gaagtttgta atgcaataat
     7561 gtggtatgtt atggaagctt gtaacactga ttgtgtactt gctcctgaat ttcaagaagg
     7621 tggattttat aaagtaaagg atgctttaga aatgttatca catcataaag agcaagcttt
     7681 agtagaagta tcttataaaa agtttaagga attaaagaaa ttagtttcag agggcgatga
     7741 tgttccagca aattaattgt gaatttagag gattctgagg aagggatagc cccttccttt
     7801 tttaattgaa gcagttttaa gcgtaaatta tatagtctct ttaaactatg atttatcaat
     7861 ttagagattt agtagtttat atttaaaatc agtaagatat atttaaaata aaaattaaaa
     7921 agtatacaat tttattatat aattgtctta tgatgttgat taaagaatta ttaaaagtcc
     7981 atttattttt aaatagaata attttataaa tatatatttg atgtgtatat tagaaaatga
     8041 ttaaaatagg aggaaataca atgagcaatt acaggacatt acatgaattt ggaacggatg
     8101 aaataacgat tgaaaagtct gtttttattg gatatgctaa gcctataaag agtgaagaag
     8161 aagcattaga gtttataaat gaaataaaga aaaagcataa agatgcaact cacaatgtgt
     8221 gggcttatac tgtaggtaaa aatatgaata tacaaagata tagtgatgat ggagaaccac
     8281 aaggaacagc tgggatacct acattagagg ttataaaaaa agaagatttg agagatgtag
     8341 tggttgttgt tactaggtat tttggaggaa caaaattagg tgcaggtggt ctagttagag
     8401 cttatacaaa aggtactaaa ctgggacttg aagcaggtaa gataatttat aaggtaatgt
     8461 atcaagaagt taaggttaag atagattata ctcaacttgg aaaagtgcaa aatgagttaa
     8521 tgaatttggg gtattttata aaagatactg tatatgagga taatgttgaa atagtagtat
     8581 attcaagatt agaagatgta gagaaattat cagaaaaaat gattgatata acaagtggaa
     8641 caggaaaaat agttttagga gaggaatttt atttgtctga acaaaatgga gaaattttaa
     8701 tataatctaa aaggttatta gaatagttaa tacctcaaat agccaattag tatttttaag
     8761 tgaaaagcca ttaaattgat ttaatctaaa tcaatttaat ggctttttat cggattttgc
     8821 acagaatcct ttttttactt caaataaacc aactaaaagt aaaaatatcc caaatatttt
     8881 ttttaatata tctgaagaca gataattagc taatagtgaa ccaaatacag ctcctaaaac
     8941 cccaaacaca gctataggta taaccaattt aaaaattaca tttttatttt ttatatgtat
     9001 agcaagtgct ataagtgtca tagggataga tgaaagtaaa tttacgcttt gtgctatttt
     9061 agtgtctatg ccagcaagta aaacgagtgc aggtataagg attgttcctc cacccattcc
     9121 cattcctcca ataattcctg caaaaaatcc aattagagca aataacatta aaacaccatc
     9181 cttaatgctg caattatcat aattattcca aaagaaattc taagaaactt tccagtaacc
     9241 ttatttagta atttagctcc tataatacca ccaagaatac ttccaactgc aactttaatt
     9301 gttaagttaa catcataagt acctttagat acatataaaa cagaacttgc tgtcgttaag
     9361 aatattatta tagaaagagc agttgcatga gatttatgtt cttctacctt aacgatgtca
     9421 tttaatattg gaactaaaag agtacctcct ccagaaccaa aaactccatt tataaatcct
     9481 gtaaaaactc caattatcgt attttttaag ttaaaatcaa agtatttttt ctttaaatca
     9541 tcagtttttt tcataaaaat caccttctga aatagtttta ccaaaatagg caaattatat
     9601 ttatataaaa taagattaaa tataatcttt catgtttgtt taattttgca taaaaaaaaa
     9661 catattaaaa caacatatta atatatggta taatatatta aaacaaagta gcaaatttga
     9721 gaggagataa caatgtcaaa tataaaaata caaatagaca aaactgtaga atggcttata
     9781 aataaggtta atgaggcaaa tgcaaaagga ttaattgttg gtgtatctgg aggaattgat
     9841 tcggcagtag ttgctaattt aattaaaaag gcatttccag aaaactctat gggagttata
     9901 atgagcataa aaagtaatcc tcaagataga gaagatgcac ttaaggttat agagggatgt
     9961 gatatagaat accttgattt agaccttata gaaccacaaa gtgctatatt agacatggta
    10021 gtaggaaatt taaaagataa acatttatat agagaagaat atttaaagat gacagatgca
    10081 aacttaagag caagagtaag aatgagtaca atttatacaa ttgctaacaa tctaggatat
    10141 ctggttgttg gtacagacaa tgctgcagaa atacacacag gatattttac taaatttggt
    10201 gatggtggag ttgatatttt acctattgca aatttaacta aaggagaggt gtatgaatgg
    10261 gcaaaagagc ttggtgttca tgaagattta ataaacaaag ctccttcagc aggtctttgg
    10321 gaaggtcaaa cagatgaaga tgaaatggga actacttaca atatgataga tgctgtgtta
    10381 gaaggtagat tggatgaagt tcctaaaaga gaccaagaga taattgaaag acttcataga
    10441 ttaagtgaac acaagagaaa aacgcctgca caacctccta agttctaatt tactatttat
    10501 aagatttgtg ataaaattta caagtgatga aaattagaat aattaattgt acgtattaag
    10561 gagtggaaat atgggacgta taggtaatat aattaataga aaaggtaaac aagatgctca
    10621 aagagcaaag atatttacta aacatgctag agcaatagca gttgcagcta aagaaggtgg
    10681 agcagaccca gagtacaatg cagcgttaaa aacagctata gaaaaagcta aggcagataa
    10741 tatgccaaat gataatatag atagagctat cgctaaaggt gctggagcag gtgctggaga
    10801 agactatgaa actatagttt atgaaggata tggacctggt ggagttgctg ttatagtaga
    10861 aactttaact gacaacaaaa acagaactgc tggtaacgta agatattatt ttgacaaaaa
    10921 cggaggaaac ttaggaacta gtggatgtgt gtcttttatg tttgataaaa aaggacaaat
    10981 attagttggt ttaggagatg gagtttcaga agaagaattg atggatgttg cattagaagc
    11041 aggagctgaa gatttcataa cagaagaaga tggttatgaa ataataacta caccagaaga
    11101 tttttctagt gttcgtgatg agttaaaagc taaaggatat gagtttatat ctgctgatgt
    11161 gaagatgata cctcaaacta caactgtatt gactgaagaa tctcatttaa aaatgatgaa
    11221 taaattagtt gatatgcttg aagaagatga tgatgttcaa gatatatatc ataactggga
    11281 agtagaataa atagtataag catattgaaa tttataagta ttgaatcaat tttaacatgt
    11341 gataaatttt atagaataag aaacactaaa atagacatac gcacctctaa cctatatttg
    11401 cataataata tgtaagcata ggtaaggggt gtttttatat ttataggtta agaaatttaa
    11461 agtaaatttt gagctggact cgattgtcta taaataattg atgggtatat aacttgttgc
    11521 tgaataccat ttatacgccc agttactgta tcaataatca tcctaagact acgttctgct
    11581 gtctcttcca tttttaaatc tataactgtc aaaggaggaa tacttcttgc cattagtcgt
    11641 gaaggtccat tacttgctgc aaatatttga atatcctctc caattacaat tcccgattca
    11701 tgaaatgcat tcatcatacc aagagctacc acatcatgat tacaaaaaat tacttttttt
    11761 agtgaatttt ttgcaagcat tttttgagca atatcatatc cactatctat tttattagaa
    11821 caataatata tagaatctga tagatgtatt ccatgttctt tacatgtttt taaaaaagat
    11881 ttacccctta aagtcataca tgtaaaggaa gttgggttga atacaacagc aatatcattt
    11941 ttaccatttt gaatagcatg taaagctgct atacgaccag tttcctcatt atcaatagat
    12001 acacttgaaa aaccaggtat ttcacgattt acaacaacta caggaatttt aggtttattt
    12061 tttactagat attctatatc atcagcattg atacctgtta aaacaattcc atcataataa
    12121 ttatttgcaa aaaagtattc atcatcacat agatgaccaa ctttgtaagg tcgtatgatg
    12181 atatttatgt ttaaatgttc taaagaaatt atgtggttta ttgcttgaag tatattggtt
    12241 gatgccaact caaatccctc atcctgccaa taaaaagcta ttttaatatt attatgttta
    12301 ataggagtag agcgtttata tccaagctta tcagctgaat ttaatataag ctcttgtgta
    12361 tgtagcggaa tcctgcgttc atttccttta ccacttaaga cgtacgagac agttgctgaa
    12421 gaaagaccac agtcttttgc aatatctttg attgttgcca tgataaaatc ctccttaaat
    12481 agttgcttaa atttgcttat accaataatc ttaacatata ttcaactaaa gaacaatatt
    12541 ctacaaggtg catattttac aggattagaa taaataaatt aaaaaaaact tcttacttat
    12601 gttgacataa tttagtttat aggattataa tatgcttaaa taagcttatg attaacttaa
    12661 attaagcaaa gttaagcagg aacatgcaat gctagcttgc aatgtgactg taaaggggga
    12721 aaaggtatga gtaaggtaaa tatatttgaa gttggtccta gagatggatt tcaaaacctg
    12781 aaagagtttt tagaaactga taaaaagctt aagattattg atggtcttat tgcttcaggg
    12841 attaaacaac ttgaaattag ttcatttgta agcccaaaag caatacctca gatgaaagat
    12901 gcaaaagaag tagcaactta ttgtgtagaa aaatatcctg atgtgaaact ttatgcatta
    12961 gttccaaatt tatacggtgc taaagttgca tgggagtgtg gtataagaga tatatcctat
    13021 gttatatctt tgagtgaaac tcataataaa aaaaatataa atcgtaccca tgaagagtct
    13081 attgatgagc ttaaaaaaat aatgaatact tatccagaca tgaatatatg tattggaatg
    13141 gctactgtat ttggatgtcc ttttgaaggt ttaccaaaaa ttgaagatgc cataagattt
    13201 gctaaacgcc tttgggaaaa cggtatcagg gagataaatc tagcagatac aatagggatt
    13261 gctgacccta agcaagtaag agaaactatt agtgcattag ttgaagcttt accagaatgc
    13321 agatggcaag tacatattca tgatactaga aatatgggaa tggttaatac tcttgtggct
    13381 attgaatctg gaattactac tgttcagtct acacttggag gattaggagg atgcccattt
    13441 gctcctggag catctggcaa tactgcaact gaagatttag tatatatgct tgaacgtatg
    13501 gggtatgata caggagttga ttttgttaaa cttcttgaat tatcaaagta tcagaagagt
    13561 tttgtaaatg gagtatatag tggtcatcat gtaaatataa agtctgatag ttgtaattgt
    13621 tagttctatt gattaatcga ttaaaaaaat atgctgattg gtaaaataga tttattgata
    13681 aaaaatataa attataaaat acattatcta ataaaacaaa taaaaaaatt gagggggatt
    13741 agtatggata ttatagcttt acttttattt ttaggagtta ttattattgc atttgtacgt
    13801 aagaataatg caggtatttt ggctctagct attggtgtca tagctgttag aatttttgga
    13861 atggatgata aagaacttta tggagctata agcaactcaa tgtttactac acttgttggt
    13921 ataacactac tctttgcaat agtgaactca acaggagcat taaagcttgt tgcagaaaaa
    13981 attgtagctg cttctggtaa acgtgtttgg cttataccta tagcagtttt tatagcagga
    14041 tttgttgttg ctggtgttgg acctggggca attccagcac ttgcaattat cccagcaatg
    14101 gctgttcctg tagccataac agttggttat aatcctgtaa tgcttgcact tataggtgaa
    14161 tgtggattaa tggcaggtcg tatgactcct attactccag aaggtcaaat tataacaacc
    14221 gttgcagaag gggttggaat tgaaaatgtt atgcctacta tcttagcctg tcaaactctt
    14281 tctacaattg tattttcaat agtattgttt ataattttca aaggatataa acttaaaaaa
    14341 cctataaatg ttatagatag taagcaaata gaaaaattca atataaagca gataatttct
    14401 ctaataagca tagtagttat gattgctttt atcataattt ttgatatgaa tgttgggctt
    14461 gcagcttttg cagtttcagc tgtacttatt tttttcaata ttgctgatga aggagaatgt
    14521 atcaaatcaa ttccatggag cacgatagta atggttcttg gagtaggtgc aatgatgaca
    14581 atcgtagatg cagcaggagg tattgacttg atgagtaatg cattatcatc actgatgaat
    14641 agtaaaacag ctactccaat tatgagtatt tcagcaggac ttatgtctat ggttagttct
    14701 gctttagcag tagtttatcc aactatgatg cctatgtgtg ttgatatagc taaggcagtt
    14761 ggaggagtag acccattagc acttattgct gcagttggtg ttggaggttc tcttgctggt
    14821 gtaagcccac tttctactgg tggggcattg attttagcag ctatgggttc aagtaagaaa
    14881 gactttaata aggaagaaca aaataaggtt tttgtacagc ttttcattat gagtgcagtt
    14941 ggcttattgg ttattgcagt agtatcaata ttatcattta atgcaataac acatttaatg
    15001 aactaattag aataactgaa aataaatcat taaatttaac ttttaatatt aatcaatata
    15061 atataatttg gaggggaagt tatgaaaaaa ggagcattaa gcaatataaa ggtacttgac
    15121 cttacaagag ttcttgcagg tccatattgt acaatgatgt tggcggattt tggagctgat
    15181 gttataaaaa tagagatgcc aagaaaaggt gatgatacaa gaggttttgg accttttgta
    15241 aatggagaaa gtttatacta tgcaaatata aatagaaata aaaaaggtat cactctaaac
    15301 cttaaatcag aagaaggaaa aaatatattt ttagaaatgg taaaacatgc tgatgttgta
    15361 gtagaaaatt acagaccagg tgtaatggaa aagttaggtc tgggttatga tgagttggct
    15421 aaagttaaca atcaaataat ttatggagct gtatcaggat ttggatgtta tggaccatat
    15481 tcagaacgtc caggttatga tattattgca caggcaatgg gtggtcttat gagtataaca
    15541 gggcaagaag gaggagaacc aacgagagtt ggtaatgcta tgggtgatgt actaggtgga
    15601 atgaatttaa ctattggtat actcacagca ctcaacgcta gaaaaataat aggaaaagga
    15661 caaagagtag atgtttccct cgttgattct gttgtatcgt ctctcgaaac tggtatacag
    15721 agatactttg attcaggaca acctgaactt atgggtaata gatatgcttc agcctatcct
    15781 tatgactctt ttaaggcaag tgatggaagt tttgtaatag gctgtggtaa tgacaaactt
    15841 tttgaaaagt tatgtaaaaa agttcttttt agagaagacc ttttaataga tgagagattt
    15901 aaaacaaatc ttaatcgttg tgaacattac aaggctctta aaccagaaat agaaaaatgg
    15961 tctgttaagc atacaataaa agaagcagtt gacttaatat cagaggctgg agttcctgct
    16021 gctcctatct ataatcttga ttgtgttaca acagatgaac atatagcaaa agcaagagaa
    16081 atgattgtag atattagtca tcctattata ggaaatatga aagtcaatgg aaatccagtt
    16141 aagttgatga caacaaaagc taaaattgat atgccagcac catctctagg ggaaagtaac
    16201 gaggagattt ataatggttg gcttggtttt gatagtgata aaataaaaga tttaaaagaa
    16261 aatggagtta tttgattaaa aaataactaa acacacttgt gactttagtc atgggaggtt
    16321 cagttagaat aatatcaaaa aatattaaat caatactgta aataaaatat agaattgtca
    16381 gaagcattta agatgggaaa agctacttag acattgagtc tttgtggctt ttcctatttt
    16441 cattaagaat tttatattat cagaatagat atgtactaaa ctaaagtatt aacaatttaa
    16501 attaatagaa aataaactac ctaattatgt tgaataaaga ctactttagt gaaaataaac
    16561 ttatagagat gatatttaaa ttattttaat ggaaatatca aaaatgagac tgcattctca
    16621 aaaatgagac tgcgtttttt aagtattttc tcgaaaatga gacaaaaata ttgttttatg
    16681 ggttttattg aagaaaaatt cttctgtgaa ttacaattta atcaatttta aagttgaaaa
    16741 ttattaaaaa tttagtgaaa tttatgtata tttcgtttat tttattaaaa ataacaattt
    16801 tttactaaaa ataaagaact tcaatggtga ttggcatagt acttgctatt ttatattaac
    16861 aagaaatgta gaaaaaataa caatgtataa tggggggaat atgatggaat ctgtaatgga
    16921 aaatttgaac aatcttaagg tagaggttaa ggacaaagtc tgtgttatta ctataaatag
    16981 acctaaagct ttaaatgctc ttaatagtga cactcttagg gaactatcac aagttattga
    17041 tgtagtatca gaaaatgagg caatattagg agttattatt actggagaag gtaaagtttt
    17101 tgtagccggt gctgatatac gccagatgca aaactataaa agtgaggaag gcagaaaata
    17161 tgcaggttat gctcagggaa tttttgataa aattgaagct cttgagaaaa cagtaatagc
    17221 agcagttaat ggatatgcat taggtggtgg atgtgaactt gctatgagtt gtgatattag
    17281 aattgcatca gaaaaggcta tatttggaca acctgaagtt aatttaggag ttattccatg
    17341 ttttggtgga actcaaagat tatcaagact tgttggaact ggaatagcta aagagttaat
    17401 ttttacgggg agacaagtaa atgcagaaga agctaaatct attgggctta ttaataaagt
    17461 ggttccaagt gatttacttt tagaagagtc tatgaagatg atgaaccaaa tagttgaaaa
    17521 agcgccaata gcaataagat atgcaaaagt tgttattaac aaagggatag atatggattt
    17581 aaagaatgct cttgaactag agaaagatat tgcaggttta acttttgcta caagagataa
    17641 gcaagaagga atgaatgcat ttatagaaaa gagaaagcca gtttttgaaa ataagtagct
    17701 attataacta aaattggaac aatgtagaat atattcaaaa tctaaatttc gtgaaaaaat
    17761 acatatagtt taattttaaa aggagttaat ttatgaatac gggagtaatt ataagtttag
    17821 tagggattat catagctcta tctcttctag ttgtaatggt tatgaaggga ataaatattt
    17881 ttattatagc aattgtatgt tcatcattag ttgctataac aggaagtcta aatgtgtatg
    17941 atgcacttaa agtggattat atgacagggt ttgttggatt tcttcaggca aatttcttta
    18001 ttttcctaac agggacatta atgggaaaaa taatggaaat tactggtgga gcaaaatcta
    18061 ttgctaagat gattgtaaga tggattggga aggataaagc actactttct attccaatag
    18121 cttgtggaat attagcctat ggtggagtaa gtgttttcgt tgtaagtttt gcagtgtttc
    18181 cgatagcttt agaaattttt aaagaagctg atttgccacg aagatttata ccagcagctt
    18241 tgacttttgg atgctcaact tttgctatgg ttgcaccagg ggcaccacaa attcagaata
    18301 ttgttccagc atcaacatta gggacagata ttatggctgg agcagtaaat ggttttatat
    18361 catgtgcagt aatgtttata gttggaagta caattttgta caaaatggtt tcaaaagaaa
    18421 aagccaatgg tggtcatttt atagcacatg aatcagatat atttgatgaa gcagaagcaa
    18481 ctaattctaa gagagaaaat ggtcccaatg gtgtaattgc cttaattcca ttgatagtgt
    18541 caattttaat aattaatgta aaagttaatg gaaaagcgat agtaccaatt gaggtgggag
    18601 tatttattgg agctttatta gtatatttat tgttaaataa atatcaagat aatactaaaa
    18661 tagttggaca tgttggtgat gcatgtaaaa ctacagtagt agcaatatgt aatacttgtg
    18721 ctgtagttgc atttggttca gtagtaaaat cagcagttgg ttttgacttc atagttaatg
    18781 ccatgactaa tattcctggt cctcctattg caggagctgc agttggaact acagttattg
    18841 caggtatttg tgggtcagct tcaggtggac taggaattgc agctccactt cttggaccag
    18901 tatttttagc caagggtgtt tccactgcag cattaactag gactatggca atttcatcag
    18961 cagcattaga ttcacttcca cacaatggat atattgttac tgtaacaaat ggtctgtgta
    19021 aagaaacaca taaggatgcc tatatgccaa tattctgggt aactgtttta actccgttta
    19081 ttggaaccat agtagcagtc ctactgttta caatgttccc gggattgcca taatagaaaa
    19141 gataaaaggt ataaattata ttttgatgtt ataaaatcaa ttttcaaaat atatacaata
    19201 aatttcatgt tttaatactg taaatatgta tttaaaataa caaattgacc atataaggag
    19261 gaattgaaag tgttaaataa accattagag ggagttaagg ttattgacct aacgtatttt
    19321 gtagcaggtc cagggacttc taaaatatta gctgactggg gtgctgacgt tattaaggta
    19381 gagccaagct ttggagaccc aggaagaaag acaggggcaa caatgacaat gccaatagat
    19441 gattataata atccttttta cagcacatat aattcaaata aaagaggttt gtctataaat
    19501 cttaagagtg aaactggaat tgaaattatg gataagcttt taagcgaagc aaacgtattt
    19561 gtttcaagtt atagaacagg agcattaaaa agactaggtc ttgattatga atcattaagt
    19621 aaaaagcatc cacaccttat ctggggtcag ataaatggtt ttggcgattt tggacctgct
    19681 aaggataatg caggatttga tacagttgcc ttttgggcaa gaagtggggc tatgcttgat
    19741 atagcagaaa aagatacaag tccaataaat ccacctatag cctttggaga tgcaacaact
    19801 tcttgtagtt tatctggagg aatatgtgca gctttgtacc agcaagctaa gactggcaaa
    19861 ggacaaaaag taatggcatc tttatttggg caagcaatat ggaatgcatc atctttatta
    19921 gcatctacac agtttagtga tgagtatcca aaaactagaa aaaatgctat ttcaccagta
    19981 ataaattctt atcgttgtaa ggatggaaag tggatattct taagtatttt agagcatgaa
    20041 agatattttg aggcactttg tagattattt ggaagagaag acttaataaa agaagagaaa
    20101 tttgcatctt cagtagaaag taagaaaaat gccacagaac ttattagtat tttggatggt
    20161 gaatttgcaa agtttactca aaatgagatg gttgaaaaac ttactgctgc tgatatagct
    20221 catgaaaaaa ttcaacatgt taatgatgta gtgactgatg aacaggctat tgctaataac
    20281 tatatatatg aatatacaag taaaaatggg aataagacaa tgcttgcgtc tactccagtt
    20341 aaatttggaa atattgaagt taacatgact tgtgatgctc cacttatagg agaacattca
    20401 gatgagatat tgagagaact tggatacagt gattctgata tagaagctct aattgaatct
    20461 ggaattgtaa caatgaataa agaagcatgt gaaatatgtt aataaacatg tgaagtatgt
    20521 taataaatag gagggaattt tatggatttt agattaacag aagcacaatt aatgcttcaa
    20581 agagtagcaa aagaatttgc agagaatgaa atagcaccaa tagctgctga aacagacaaa
    20641 acaggtattt ttccaaggga gctttttagc aaaatggcta aaataggatt taatggaatt
    20701 ggaacacctg tagagtatag tggttctggt ggggcagata tagaaaaggt aattgttgtt
    20761 actgagattg ccaaaaaatg tgcagcatct gcagcaatat tatcaataca cacaatatat
    20821 gctcaagcta ttttgaaatt tggtactgag gaacaaaaga aaaaatacct tccaatgatg
    20881 gctgaaggtg ggtgtgtagg tgcttttgcc ttaaccgaac ctaatgctgg ttcagatgct
    20941 gcgagagctg caacaactgc aattattgat gaagaaactg atgaatatgt tttaaatgga
    21001 actaagtgtt ttatttctgg tggaggtcaa gcagaatcat taataatatt tgcactgaca
    21061 gacccatcaa agggaataaa gggaatgtct gctatcattg tcgacaaagg tactccaggt
    21121 ttttcgatag gaaaaattga agaaaaaatg ggtattcatg gttcagaaac tgcagagtta
    21181 atatttgata actgtagagt tccaaagtct aacttattag gaaaagaagg caaaggattt
    21241 aatatagcta tgacttgttt agatggagca agaattggag ttggagctca agctgtagga
    21301 attgctgaag gggctttaga agaaagtatc aaatattcta aagaaagagt ccagtttggc
    21361 aagccaattt cagctttgca aggtattcaa tggtatatag cagatatggc gacaatggtg
    21421 gaatcagcaa aattattagt atattatgct gcagatttga aagctagagg agaaaaacac
    21481 actaaagaag ctgctatggc aaaatacaat gcgtcacgta ctgcaagaga ggttacaaat
    21541 ttagcccttc aaattcatgg tggatatgga tatatgaagg attatccatt agagagaatg
    21601 tacagagatg ccaaaataac agaaatttac gaaggtactt cagaaataca caaagttgtt
    21661 atttcaagag cagtactagg ataggaggaa aagaatgaat atagttgttt gcttaaagca
    21721 agttccagat actaatgaag ttaaaataaa taaagagaca ggtactctaa taagagatgg
    21781 tgtaccaagt ataatcaatc cagatgatag aaatgcttta gaagaagctc taaaaatgaa
    21841 agatgaacta ggagcagtga ttaaggttat aagcatggga cctcctcaag ctaaaagtgc
    21901 tttaaaagaa gctttggcaa tgggagctga tgaagcttat ttaatctcag atagagcttt
    21961 tggtggttca gatacttggg ctacatctac aattattgct gctgctatag agaaggttgg
    22021 aaagtatgat gtaatattct gcggaagaca agctatagat ggagatacag ctcaggttgg
    22081 accagaggtt gcagaatttt tagggatacc acaagttact tatgcgaaag aagtaaaggt
    22141 tcaagatgac aaattactag taactcgtta tacagagaca ggggattatt taatagaagc
    22201 aaagatgcca gttttattaa ctgctataaa agagttaaac aatccaagat atccaagtgt
    22261 caaaggaatc ttagaagctt ataataatgg agatgcaaag attactgtat taacacttgc
    22321 agatttagat gttgatacaa ctcaaattgg attaaagggt tcacctacaa acgtttataa
    22381 gtcttttgta ccagttaaag acaagcataa tgaaataata gaaggtataa ataaaaaaga
    22441 aaaagctgag aaactaatag aaatactatt cgatttgaaa ctagtatagg agtgttaaga
    22501 tgatgagagc aaaagttaat caaggtataa atttaaatga ttataatggt gtttgggtaa
    22561 taggagagca aagggaaggt aaaattaatc ctgttacgat agaacttatt ggtgaaggaa
    22621 gaaagcttgc tgaccaatta ggaaaagagc ttgcagttgt gatagctgga tatgaagtag
    22681 agaaagaagt gaaggaatta cttcactata gtgttgataa agtatattac atcaatgacc
    22741 ctcttcttaa agatttcact actgatggat atgcaatttc aattgcaaat ttaatcgaaa
    22801 gaaaaaagcc agaggttgta ttggttggtg ctacttctat aggaagagat atagcgccta
    22861 gaattgcagg taaggttgga acaggtctaa ctgctgactg tacaaaactt gaaatagatt
    22921 caacagataa taagttatta caaacaagac cagcatttgg tggaaattta atggccacta
    22981 ttgtttgtcc aaaaaataga ccacagatgt caacagttcg tccaggagtt atggcaaaag
    23041 ctgtaagaaa cgaatcagag actggaattt tagaagttgt tactccagaa cttactgaaa
    23101 aaatgattag aactagatta gtagagatac ttccacaaga aaagaaatct gtaaatctaa
    23161 ctgatgctag aataatagta tcagggggaa gaggattaaa aagagcagaa ggatttgagc
    23221 ttataaaaga attggctgat aaattggggg cagagattgg tgcttcaaga gcagcagtag
    23281 attcagggtg gatagaacat tctcatcaag tgggacaaac aggaactact gttagaccag
    23341 aactatacat tgcatgtgga atatctggag caatacaaca tttagcaggt atgagtgatt
    23401 ctaaatatat agttgctata aataaagatg caaaagcccc tatttttagt atatgtgact
    23461 atggaatagt aggagattta tatgaaatta tacctgaaat gatagaatca ttaaataggt
    23521 aacttttaga aaacattgaa tatgtagaat gataagtctt ataatattgt attgtaagac
    23581 ttattatttt tgctgaaggt tatagtgggg agatgaaaca atgaaagagc agtggtataa
    23641 ggatatattt gcaagagtat tatctatgac tgatgatggt tttattgttg taaatacaag
    23701 tggagtaata atagatatta atgataaata ttgtgacttt ttaggaaaag aaagaaaaga
    23761 tataatagga caaaatatac aaagtattat accaaataca aaaatgctag atgttatgaa
    23821 aaataagtat tgtgaagaag gagctataca tcattattca ggtggaaata caaaagaaaa
    23881 aagtgtaata gtaagtaggt cttatgtgga aaatgacaat ggagaagtag ttgcaggagt
    23941 agctcaagtt aaatttagat tgcaatcatt tgatgtagct aaaaagttaa tgtcagaata
    24001 tatggagctc caatattata aagagcagtt taaggataac tgtggttttg acaagctcat
    24061 aggtgaaaat agagacttca tagaacttaa gaaaacaggt gtgaaagctt ctaaaacaaa
    24121 ctttccagta cttttaacgg gtgaaacagg cacaggtaaa gaagtttttg ctagagcaat
    24181 tcacaataat agcagcaggt cagataaacc tatggttagt ataaattgcg ctgcaatacc
    24241 agaagaatta ttagagtcag agctatttgg ctatgacgaa ggagctttta caggagcaaa
    24301 aaagggtggt aaaaaaggta aatttttagt tgcgaataat ggtacaatct ttttagacga
    24361 gataggtgac atgcctttga ccatgcaagc aaaactttta agagttcttc aggaaagtga
    24421 gatagaacca gtgggaggtc ttaaaactat aaagatagat gtaagagtaa tctctgccac
    24481 aagaaagaat ttaagtaaaa tggttgaaga gggattattt agagaagacc tatactatag
    24541 attaaatgta ataaacattc atatgatgga attgaaggac aggcaagatg atattttatt
    24601 gctagcaaat tatatattaa acaaattaaa tgttgaatac aaggaattga aggttttaag
    24661 tgataaggtt aaaaattgtt ttattaacta tacttggccg ggaaatataa gagaactaca
    24721 aaatgtaata aaaagtgcct atgctgtaag tgatgatatg gtcattatga tgtgtgacct
    24781 tccatctaag atggataata tcagcagagt agctcaatgt aatgtggata gcaattgttc
    24841 aattcatgaa atggttgaga attatgaaaa gagcttaatt attgatgtgt taaggaagta
    24901 taattggaaa tgttctaagg cggcagaggt tatgggaata cacaagagtc ttttatataa
    24961 gaaaataaaa aagtatgaaa ttgaattaaa taattaagat aaattgaatt aaggcaatgc
    25021 taaaaaagcg cccttattca aagcgaactg tataaactgt tttatgcagt taaaacgtgt
    25081 aaaagtagat gtgaagtact aatgtcaatc attagtactt ttttattata tttataatta
    25141 ctatctatat ttcagtatct tttgatatct aagattatta tgtttaattt aggatataac
    25201 aaaaccctct tagttatgga cagtttttta tattttaact gtctactaag aggggatagt
    25261 ttagtttaaa tttagagtaa attttatcta gcatttataa aaaatatttt gaatagcttt
    25321 tttgttattt agtcataatt ttctacttct ttatcaagtt ctttgccaca agaatcacac
    25381 ttatatatag cattgtatcc gtaatcatat tcatcaacta ttttcttttg tctaaataca
    25441 ccaccacact cacataggaa accaattttt ataagtgtaa cgataaattg tgattttata
    25501 aattttatat cattgcattt atcgttagtg taaaatgtat aaatataaga gttatcgcac
    25561 tcatctctta attcaaatct ttcaatatat gtgttatttt ttaattcctc aaattcttct
    25621 ttagttaaat atgatttttg ttgcaattca tgtaaacgct taatttggtc cattatttat
    25681 atcctcccaa taattttagt attttaagct tagataagga atctaagttt tattaaattt
    25741 atcccaagga attatttaaa tcatatttag ttttattaat tacaacatat ataaattaca
    25801 cgaaaataaa actaattgta aaaataatat cctgtgaaaa cctaaactac taacccctta
    25861 ctaacccgaa aactaaaaaa agaaaatata tccaaatata agtg
//
LOCUS       DACXMZ010000031        25833 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.031, whole genome shotgun sequence.
ACCESSION   DACXMZ010000031 DACXMZ010000000
VERSION     DACXMZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.031"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..429)
                     /locus_tag="JD016_05060"
     CDS             complement(<1..429)
                     /locus_tag="JD016_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAU4999001.1"
                     /translation="MQEETNEKTIALYIKTGKLTAQQLQKAMKALLAQMKKQHDKQKI
                     PHGKQTLKQLMKQNAGVSNIEITKDNIKAFESTAKKYGIDFALKKDSTETPPRYLVFF
                     KGRDADALTAAFKEFSAKKLTQEQNPSIRKLIVSLKEKAAA"
     gene            complement(524..742)
                     /locus_tag="JD016_05065"
     CDS             complement(524..742)
                     /locus_tag="JD016_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999002.1"
                     /translation="MTQKTGALIFDETADRYDIRFDVNDYYGGLHCGDCMEVFVRGKW
                     KPTRMEYGDNWYLVGIRAADLSGLRVRI"
     gene            complement(767..1711)
                     /locus_tag="JD016_05070"
     CDS             complement(767..1711)
                     /locus_tag="JD016_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU4999003.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKELSLKAKGLLSQMLSLPEDWD
                     YTLAGLSLINRESIDAIRTAVWELEKAGYITRRQGRDEKGKMTAIEYTIYEQPQPPAL
                     DCPILENPTADNPTSENPTQLNKELLKTNLPNKEKLNTDISSTHSIPFHSLNPSPLED
                     AAQPPERKRKETTDAYSVYEEIIKDNIEYDYLIQDRYLDRDRIEEILALILETVCTKR
                     RTIRIAGDDHPAELVKAKFMKLNSEHIRFVLDCMQENTTKIRNIKQYMKAALFNAPST
                     IGSYYTSLVSHDMADGTFYGKPKKSGIPDYTCDEGESL"
     gene            3099..3977
                     /locus_tag="JD016_05075"
     CDS             3099..3977
                     /locus_tag="JD016_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAU4999004.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            4013..5908
                     /locus_tag="JD016_05080"
     CDS             4013..5908
                     /locus_tag="JD016_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HAU4999005.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            6176..8083
                     /locus_tag="JD016_05085"
     CDS             6176..8083
                     /locus_tag="JD016_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAU4999006.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDELMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            8073..8522
                     /locus_tag="JD016_05090"
     CDS             8073..8522
                     /locus_tag="JD016_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAU4999007.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            8622..9509
                     /locus_tag="JD016_05095"
     CDS             8622..9509
                     /locus_tag="JD016_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAU4999008.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            9914..11362
                     /locus_tag="JD016_05100"
     CDS             9914..11362
                     /locus_tag="JD016_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAU4999009.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MSYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIKTDGEILFNDMETHA"
     gene            11388..13778
                     /locus_tag="JD016_05105"
     CDS             11388..13778
                     /locus_tag="JD016_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAU4999010.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSDITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDVSRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            13780..14451
                     /gene="pgmB"
                     /locus_tag="JD016_05110"
     CDS             13780..14451
                     /gene="pgmB"
                     /locus_tag="JD016_05110"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAU4999011.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLA"
     gene            14490..15890
                     /locus_tag="JD016_05115"
     CDS             14490..15890
                     /locus_tag="JD016_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAU4999012.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASI
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKEGIDNVVEEV"
     gene            16186..17049
                     /locus_tag="JD016_05120"
     CDS             16186..17049
                     /locus_tag="JD016_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU4999013.1"
                     /translation="MLKRRIIMIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGN
                     LIDKTKVNKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYI
                     SLSDHTYSAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEA
                     GFIAFHIVNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFF
                     AQRLLLDSAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTI
                     HLAKVVKDSNM"
     gene            17194..19122
                     /locus_tag="JD016_05125"
     CDS             17194..19122
                     /locus_tag="JD016_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit EIIBCA"
                     /protein_id="HAU4999014.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            19177..20610
                     /gene="ascB"
                     /locus_tag="JD016_05130"
     CDS             19177..20610
                     /gene="ascB"
                     /locus_tag="JD016_05130"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAU4999015.1"
                     /translation="MTMSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRT
                     AVITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKG
                     DEETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEK
                     LCNVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVAS
                     AIATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYP
                     NYALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFT
                     SAKNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDD
                     YRIDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSG
                     NGSLRRMKKKSFEWYKKVIASNGEDLY"
     gene            21015..21797
                     /locus_tag="JD016_05135"
     CDS             21015..21797
                     /locus_tag="JD016_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAU4999016.1"
                     /translation="MNMKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQD
                     CLMCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVS
                     SEIIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSK
                     EVEIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNS
                     PKLKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            21813..22178
                     /locus_tag="JD016_05140"
     CDS             21813..22178
                     /locus_tag="JD016_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAU4999017.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(22344..23093)
                     /locus_tag="JD016_05145"
     CDS             complement(22344..23093)
                     /locus_tag="JD016_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAU4999018.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            23285..23443
                     /locus_tag="JD016_05150"
     CDS             23285..23443
                     /locus_tag="JD016_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999019.1"
                     /translation="MYMRRDNNLLMKDYKYKGYFESVEIFDNVNDLNLNTLTTVSRFV
                     LVFLFTIT"
     gene            23571..23996
                     /locus_tag="JD016_05155"
     CDS             23571..23996
                     /locus_tag="JD016_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999020.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIC
                     SCIKLVKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            24028..25215
                     /locus_tag="JD016_05160"
     CDS             24028..25215
                     /locus_tag="JD016_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAU4999021.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            25407..25811
                     /locus_tag="JD016_05165"
     CDS             25407..25811
                     /locus_tag="JD016_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HAU4999022.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT         9913 a         2957 c         4685 g         8278 t
ORIGIN      
        1 agccgccgcc ttttctttga gggaaacaat cagtttgcgg atagagggat tttgttcctg
       61 cgtcagcttc tttgcggaaa actccttgaa agctgcggtc agtgcgtccg cgtcccgtcc
      121 cttgaaaaac accaaatagc ggggcggggt ttcggtgctg tctttcttta aggcaaagtc
      181 aatcccgtat tttttcgccg tactctcaaa ggctttgata ttgtcctttg tgatttcaat
      241 gttggaaacg cccgcgttct gcttcataag ctgctttaga gtctgctttc catgcgggat
      301 tttctgtttg tcatgctgct ttttcatctg tgcaagcagg gctttcatag ccttttggag
      361 ctgctgcgcc gtcagctttc cggtcttgat gtaaagggct atggtctttt cgttggtttc
      421 ctcctgcaat gggtttcctc ctttcgtgtt ctatggtggt atgcccgccg ccgggaagtg
      481 gggcgggcat gaaaaaaaga gcgtccgttt tcacgacgcc ctgttaaatc cgtacccgca
      541 gcccggaaag gtctgccgcc cgaataccca caagatacca gttgtcccca tactccatac
      601 gggtaggctt ccatttgccc cgcacaaaga cctccatgca gtcgccgcaa tgcaagcccc
      661 cgtaatagtc gtttacgtca aagcgaatgt cgtagcggtc agcggtttca tcaaaaatca
      721 aagctcctgt tttctgtgtc attataaaat cctcctttgg gttggtttac aggctttcgc
      781 cctcgtcgca ggtgtaatcg ggtatgccgg attttttcgg tttcccataa aatgtgccgt
      841 ctgccatatc gtgagataca agggacgtgt aatagctgcc aatcgtagac ggggcattga
      901 aaagggcagc tttcatgtac tgcttgatgt tgcggatttt ggtggtgttt tcctgcatac
      961 agtccagtac aaagcggata tgttcgctgt tcagtttcat aaattttgct tttacaagct
     1021 ctgccgggtg gtcgtccccg gcgatacgga ttgttcttcg tttggtgcag acggtttcaa
     1081 gaataagggc aaggatttcc tctatcctgt ccctgtcaag gtatctgtcc tgtatcagat
     1141 agtcgtactc gatattgtcc ttgatgattt cctcatacac gctatatgcg tctgtcgttt
     1201 cctttcgctt ccgttccggc ggctgtgccg cgtcctctaa gggagaggga tttagggaat
     1261 ggaaaggaat ggaatgggta cttgatatat ctgtatttaa tttttctttg tttggtaagt
     1321 tagtctttaa tagttcttta tttaattgcg ttggattttc cgacgtcggg ttatccgctg
     1381 ttggattttc caatatcgga caatccaatg ccgggggctg tggctgttcg taaatggtgt
     1441 actcgatagc ggtcattttg cctttctcgt cgcgtccctg ccgccttgtg atatatccgg
     1501 ctttttcaag ctcccataca gcggtgcgga tagcgtcgat actttcccgg ttgataaggg
     1561 acagccctgc aagggtgtag tcccaatctt cgggaagtga gagcatttgc gataacaagc
     1621 cctttgcctt tagggaaagt tccttgttgc gtaaatggtg gttgctcata accgtataac
     1681 ctttgttctt ttccacgcga aaaactgcca tagcttcgtc actccttttc gttggatttg
     1741 ttacgcagta gaaacgcggt ttggagggaa aaggtatcaa cccttgtctt tcttatctcc
     1801 gcgctctaag agccacataa atcaagactt cttttgccct tactgcgtaa catgagggca
     1861 taaaaaaagc agcgttcctg ttctcctgtg taggagtaag aaacgccgcc ttataggtgg
     1921 tgcttatttt attttttctt atcgacgtgc cgcgcaaggt ggtgtagcgg tctatccggc
     1981 agcttcgcag gtgcttttgc atatcaaggc gcaatccgtt aaaagtagta aatgagtagt
     2041 aaaatcaatc tgcaaacctg cgaatatgcc cgtaaatcaa gcatttttca agacaatcaa
     2101 ctaatttatt aaaaatgtaa taaaatttaa tggagaaatt ttataggtag ataaatttta
     2161 atatttataa gattatgtta aggaaacact taaaaattag attaaaattg tgggtattta
     2221 aatcaactaa aagatattga ctatttagtc aatatctttt tttattaaaa aggaatatac
     2281 atataaatag gttagattaa aaattgggat aaatatgatt tatatagcat catttaatta
     2341 aaaggagcta tattagtatt cattcataca attggataat atacaatgtc agccttgctt
     2401 tatggtgttt gtaataaacc tatttcaatt tctattgtaa ctaatatgca agaatttaag
     2461 ataggagtat ctatggatta tggtgtatta gtaattggta tatgctatat agcattagga
     2521 ataattttta aattagatag aaaaagattt acataagata acaaaggcta atttataaat
     2581 tggtgcttag aaaaccaaat cactttatta gctagagatg aatttggagg gtgtcacttt
     2641 ggtgttttat gtaaagttta tggaagtaaa acattagata atcgtgagta tgatttctat
     2701 aaatcatata tactattgga atccaagaaa aggtattggc caaatagcta atatcttttt
     2761 aatttttttg actctaatat agcattaact attgcatgag tatctttaaa tttatagtta
     2821 atgtatctta attgtttatt ataaaatcta ggaaggctaa agcataaaac acttaaaaag
     2881 agaagatgta catgagaaat ataattaact atgatgaaga cttctaaaaa ttatttagtg
     2941 aaaaggaact tagagtgtat aataaaagtt ccttttcact aaggaaaacg attgttattt
     3001 tctgaccttc attaccctga tattatttaa atataaacaa caagaaaatt aacaagaagc
     3061 aaattcaacc taaaatttaa aatatcggga ggtacaatat gttaataaac atgaaggaaa
     3121 tgctaaaagt ggcacaagaa aatcaatttg ctgtaccagc atttaatata ggaagtgggc
     3181 aaatacttaa agcagtagta cagagtgcta acgaaaaaaa tgcaccagta atacttgcaa
     3241 tacacccaaa tgagctaagt tttctaggag atagctttgt agctagttgc atagaagaag
     3301 caaataagag taaagtacct atggtaatac acttagatca tggggaaaat aaagaacaaa
     3361 tattaagggc aataagatgt ggatttacat cagtaatgat tgatggctca catttaccat
     3421 atgaagagaa tgtagctata tctagggaaa tagtagaaat agcaaaagga ttaaatgtgt
     3481 ccgtagaagg agaacttgga acaataggaa caacaggaac atcaagtgaa ggtggtactg
     3541 atgaaataat atatacagat tcaaaattag ctaaggattt tgttgaaaaa acaggagtag
     3601 acacactagc tattgcaatt ggtacagcac atggtatata tccaaaaggt tttaaaccag
     3661 agcttaaact agatttatta aaagaaataa gagaagtagt agatatacct ttagttcttc
     3721 atggtggttc atcaaatcca gatgaagaaa tagcacaagc agttaaacta ggagtttgta
     3781 aaataaatat ttcgagtgat gttaagtcgg catattataa gaagtgcaga gaactattag
     3841 aacaaaaccc aagcttatat gagccagata ctatatatcc accatgtata aaagctgcaa
     3901 gagaagttat agagtttaaa atgaatctat tcaatgctat agataagtta aagtgctttt
     3961 ataaaaataa aaattaatta tgaggtaaca aattaaaagg aggggagaat ttatgatagg
     4021 taaaatacta cgaagcgatt gtattgtact agataaacaa ttacaaacaa aaaaagatgt
     4081 aattaattat atggcaaatc atttgtacca tggaaattat ataaataata aagaaaagtt
     4141 tatagctgat attaataaac gtgaaaaaga atcatcaact aatatgggta atttaatagc
     4201 tatacctcat gctaagtcaa atgtagtaaa tcaacctgta atgatgtatt taagaacaga
     4261 tagagagatt acagataatg atggtcaaaa acttaggtca ttttttatga tagcagttcc
     4321 agaatcaggt tcagatgaac atcttaagat tatatctaaa ctagctacat atctaatgga
     4381 agaaaatttt cttaagcatc tactaagtgc agaaactcca tacgaaatat taagtatatt
     4441 taataataaa gctgcagaat caggagattt tagtaataat caaaataaag gtcttatagt
     4501 tgggataaca ggatgtccaa caggtatagc tcatactttt atggcaaaaa aagcattaga
     4561 ggaagcaggg caagaacttg gatatgaagt taaaatagaa actcatggag ctataggtgt
     4621 agaaaataag ttgactaaag aagatataaa aagagctgaa tacgtaataa tagcttgtga
     4681 taaagaagtt caacttagta gatttatagg aaagaaagtg ctacaaaaga aagtagtaga
     4741 tgctaagaat gtttcattag ctaaaaattt aattataaac gctgaaaatg gaatggctaa
     4801 agaaattaaa ccaggtggaa agaccttaga agattcaaaa gaaatgcttc aagggtcaga
     4861 gaaaaagggt gtttataagc acttaatgag tggtgtatct gaaatgattc catttgtagt
     4921 gataggtgga attggtatag ccatagcatt tatgtttggt atatttgcat cagacccaac
     4981 tcatgaaaca tacaacccta tagctggatt ctttggacaa cttggaggcg attcagcatt
     5041 taagttattt ataccgatac ttgctggttt tatagctaaa tcaatagcag gaagacaagg
     5101 atttgctcct gctatgatag ctggttttat ggcagttatt ggaggttcag ggtttatagg
     5161 tggtatggta gctggttttg caacaggata tatagcttta ggagtaatga agataacaga
     5221 aaatttacca gatgtattaa aaggagtaaa tgctgtattg atatgtccac ttttgattgc
     5281 atttacgagt ggagctataa tgttctttgt agttaatacg ccagcatcat ggttaaatga
     5341 agctttacaa tcttggttac aaagtatgtc tggaactaat aaagtattat taggtgcaat
     5401 attagcaggg atgatggcat cagatatggg aggacctatt aataaaacag cttcagcttt
     5461 tggattggct atgttttcaa gtaatatatt tgaaccatca gcagcattaa tggtaggagg
     5521 aatggtacct ccattagcga tatcattggc tatgttaata ttcaaaaata agtttactaa
     5581 agaagaaaga aagtcatctc taccaactgc tataatgggg gcatctttca taacagaagg
     5641 cgcaataccg tttgcagctg cagacccatt aagaatgatt ccagcaaata taattggttc
     5701 ttgtataggt ggggcaatat gtatgagttt agatataact ttaatggcac cacatggggg
     5761 aatatttgta ataccatttg catgtagtgc accagttgca tatatagctt gtatattgat
     5821 tggtacagta ataacagcat tgattatagg ggtaacaaag cctactataa ggagtgaaaa
     5881 tagtgaagtt gaattacttg gtgcttaaat tataaataat agtaaaaata attaatggaa
     5941 taacaacaag tatatagaat aataataagg atatttagaa tcagttaact acttctaaat
     6001 atccttaatt tttattttga aattatatta tctattatat caatttagta aggtgtatca
     6061 taaattcttt tatgatacag aagtatctta gtaattttac atagttagta aatatgttaa
     6121 aatagtactt aatataaaca tagaaatata taaacatagg tagggagggc taagtatgct
     6181 tgtgtcgagg caaattgaat ttcttaaatt gatactaaac gaaaataaat atagacctat
     6241 aaaatacttt aaagataaat taaatgtatc tgataaaact ttacaaaaag atttgaagat
     6301 gattgagaaa tatttagaaa cttttaatat aaaaattgat ataaaaagag gctatggaat
     6361 attaatagaa gatttagcaa agaagaacat agacttaatt aatagtttaa atatacaatc
     6421 gaaggaaaat aaatctttat cagtagagca gagaagaatg gagattttaa agtatttatt
     6481 gctaaattca aataatataa cttcaataaa tcagttagca gataaatatt atgtaagtaa
     6541 aacttctata gtaaatgatt ttaaatatat agaggcatgg ataaaagaat acaatttaaa
     6601 acttaataaa actttagaag gaactaagat tataggtaaa gaagtggata taaggaaatc
     6661 gattgccaaa atgatggatg atttattaga tgaaaattat gaacaacatg atatatctga
     6721 gttaacaaga ttagattctg ctacatttag tgcattgata aatttatttg atattgattc
     6781 tattatattt gtagaaacta taattacaga attagaatac aatttaggat atactataaa
     6841 tcagccctac tatataaatt taattaccca tatacttata tgtttaaaga gaatcgaaga
     6901 aggtaatcaa atagaatctc aggaagaaag agaaatttct gtagataatt tggacgagtt
     6961 agtatataaa aatgtcacta cacttataga taaaatagaa aatagataca gagtaaaaat
     7021 aacgaaagat gaacttatgt acatatatat ttttttggta tcatctggat ttagtagcga
     7081 aaataatagt agttacaatg atgaatcaga tagattaagt gaaagtgaaa aagtttcatc
     7141 tataatgata gagaatatgt cacgattttt aaatataaat ctcaaaaatg atgacttact
     7201 acagaaaagt ctagcatctc atgtaaggcc tatgttaaac agattaagat atgatatcca
     7261 aataaaaaac ccgttgttag gagagataca agagcgattt tcagatgtgc tgggattatg
     7321 tatgatgact ataaatatta tgacagagta tgataatctg aagaatatta gtatagatga
     7381 agtctcgtat cttgccacat attttcaagc ggcaatagaa agaaatatga gtagtaaaag
     7441 agtaatagta gtttgtcata caggatatgg aacatctcag ctccttgcag ccagattaaa
     7501 aagagaattt cctgaatgga ctatagttga tatagttcct atgcatcagt taagtaagcg
     7561 aaatttagat gatgtagact ttgtaatatc tactgtaaac ttagatttga aagataaatt
     7621 acatatagtt gtatcagtac tattaatgga aaatgatatt aataatataa aaaatgcttt
     7681 actttctaaa cctaaaaaaa gtatgaactt agatacaaac ttattaggaa tgtatttaga
     7741 agataatata tattttaatt ttgatgtagc tcagatggaa agtttaatag gtgtgaattt
     7801 agggagtaaa tatgaatcta tttcattagg aaaagattta aatatatata tacataagca
     7861 aagtaaatta aataaaggga taatgaatat caataatgtt actagaacga ttgatattta
     7921 tttagaaatt agtaatcaaa gttttttaaa gaatatttta atggaaatat caaatttata
     7981 taggcaaaag aactatataa actatataat agattgcaag agcaaagagg atattaaaat
     8041 attatttaag aataatctat tggagaaaac aaatggaaat tagagaaata ttaacagaag
     8101 aaagcattat tttagatttg aaatcaacta caaaagaagg cataatacag gaattagtag
     8161 ataaattata cgatacagga agtataactt taaaaagtaa gtttataaag gcattaaaac
     8221 atagagaaaa aataggctct actagtatag gttatcaaat ggccatgcca catggaaaaa
     8281 caaaatatgt aaagaaggca tccatagtaa taggtaaatc taaaaaaggt gtagattggc
     8341 caagtataga tggcttacct gtaaaggtaa taattatgtt tgctatgcca caatctggag
     8401 gcaatgaaga tatgatatta atatctaaaa tagcttacat tttaggtggt aaaaatatag
     8461 gtgaaagact tttaaatgca catacaaaaa aagaaatatt agatatattt tatgaagtgt
     8521 aggtattgta taaattataa atttgaggtt tttaaaataa aatgtgtatt cttttaaata
     8581 gtataaatta atttttagac actagagagg agaggatata tatggcaata tatttagaaa
     8641 tgcctaaatt aatacatgaa tttccttttc gtagtctatc aaatgaagga gaagtattaa
     8701 caacaccaca ctggcataag gaaatagaaa tattacttat aacagaaggt gtagtaaatc
     8761 tatttataaa tgacaagcct atgcaattaa aaaaaggcga gatagtgata ataaaaggag
     8821 gagatattca ttatatactg ccatcaccgg gaagtgaaag attagtattt caatttgata
     8881 tatctttttt taatgattta attttattaa atgaggaaaa agaaagctta attaatttat
     8941 tcagtagtat aaaaaaatgt agtagagatt ggtcagcaaa aataagaaag tcagttttcg
     9001 atatattgat agatatctac aatgaagacc tatacaaaat agaaggatat agttatgcaa
     9061 taaaaggtaa aatgtataat ttaattccta tattatatag acagattcct agagaaattg
     9121 aagaaaatac agtagaatat gaaataaact ctaaagagat attagagaga ttgaacaata
     9181 tattcaaata tgtagaaaaa aattataaac aacctataaa attagaagat gtgtcttatg
     9241 aagtaggatt tagtgtatat tattttacta aatttttcaa aaaaaataca ggaaaaacat
     9301 ttataacttt tttaaatgaa tatagattag aaaaagcaaa gtggatatta cttaataaca
     9361 atatatccat tgtagatttg gttgaagaag ttggttttgg aagtacaaaa actttttaca
     9421 gagtatttaa agaaaaaatt ggagtctctc cattagaatt taaaaaaaga tataatagct
     9481 tggagaaaaa agggagtgtc tcaaaatgaa cttagttcat gaggcacttt cttttatatg
     9541 aaatcaaata tattttcagt atttcaacaa tgaaaattcc attcatttat aaagtctaaa
     9601 ttttagttct caatacaacc tctatttcga aataaatcct tttatatata taaatataca
     9661 gcaatatatg agaataagat aacaataatg aggatataaa aatgtatttg ctatatatat
     9721 aattatatac atagatatct aatattgata tttaatatga aagtattgaa aatagacaat
     9781 attaattata gtgagtaaaa aatataattg gatttttaaa ttgaattatt taaaaggtat
     9841 agtatgaaaa aaatcttata actaaatatg agatagaaat tataaaactt tatattgtga
     9901 aagaggtaga tgcatgaaat tagggaaaga ttttttatgg ggaggggcaa tttcagccaa
     9961 tcaaagtgaa ggattttatt tagatggagg aaaaggttta tctcagtttg ataaactccc
    10021 attaaatgat aagagattaa aagatgtatg tttagatgag gataatattt taaatgaaaa
    10081 taatcaatac tatccaagtc atatagggat taaattttgt gaaaattaca aagaagatat
    10141 agcaatgtta gcagaaatag gttttaaatg ttttagattt tctatatctt ggtcaagaat
    10201 attcccaaca ggagaagaaa gtgaaccaaa tgaaaaggga ttacaacttt atgataatat
    10261 aataaaagaa ttaaaaaaat ataatataga gccattaata acgataagcc actttgatat
    10321 accttattct ctagttgaaa aatattctgg gtgggctgat agaagagtgg ttaatttata
    10381 tgtaaaatat gctgaaattt tgatgaaaag atataaagat gatgtcaaat actggattcc
    10441 ttttaacgaa atgaatatga taatgcatat tccatatata ggtgctgggt taacttttat
    10501 agctgatgaa aatcaacttc agaaaaaata tcaagcagca caccatcaat tattagctaa
    10561 tgcaaaaact atagaaatag gaaggtatat aaataaagaa tttaagtttg ggtgtatgtt
    10621 agctgctgga aaaacatatc catacacatg taaaccagaa gatgtatttg caactttaga
    10681 gcatgaaagg cataatttgt ttttttctga tgttcaagtt aggggtgtgt atccaagctt
    10741 tatgagttat tatttaaaat caaatcaaat aaaattaata ataaaagagg aagatttatc
    10801 aacacttaag gataatactg tagattttgt atcttttagc tattattcta gtgcttgtag
    10861 ttcagctaga gctgaaggtt tagaaaaatc aaaggcaaac ggaccagaaa ccataaaaaa
    10921 tccatatctt ccaaaatcaa atagtgtatg gcaaaacgac cctgtagggt taagaataac
    10981 tatgaatcag ttatatgaaa ggtatcagtt acctttattt atagtagaaa atggactggg
    11041 tactcaagat attgtaaatg aagatggaaa aataattgat gattatcgta tagattacat
    11101 gaaagaacat attaaaaata tgattcttgg agtaaatgaa gatggaatag agcttatagg
    11161 atatcttatg tggggatgta tagatgtaac cagtgtaagt gaaggaaaga tgacaaaacg
    11221 atatggcata attcatgttg attctgatga ctatggaaga ggctcattta aaagaactaa
    11281 aaaacaaagt gctttttggt ataaaaaagt aataaaaaca gatggagaaa ttttatttaa
    11341 tgatatggaa actcatgcat aattcatttt aagaaaggag tgttattatg agtttaatat
    11401 ttactactaa ggaccaactt atagagcaag ggtttagtaa ggatacaata aaatccaatg
    11461 aaacgctgtt tacactagct aatggacatt taggagttag agggaatatt gaagagtctg
    11521 attttaataa tgagtatgta gataatatgg gtacatatgt aaatggattt tatgaagaat
    11581 ctccaattac atatggagaa agcgcttatg gctatgcaaa acaaaatcag actatctgta
    11641 agttacctaa tgctcatata gtaaatttct ctattgaagg agattatttt gatttatcaa
    11701 aaggtattac aagtgaacat tctaggatac tagatttaaa tgaagggatt cttaaacgtt
    11761 catttatatg ggaaaactct cgtggtagaa aagttaaagt atctatagaa agattagtat
    11821 cacaagatat agaagaatta atgatgattt catatacaat aacaccattg aattttaatg
    11881 gaaaaataaa atttaaaaat aaaatggaaa atagtatgga gaagtttaat tgtgaaaaag
    11941 tagatgaccc aagagtggca tctataaata agaagcaatt taaaattaat gtaaaaaaat
    12001 ataacaataa gtattttatg aatttagtta cagaaagaag tagattaggg ttatggtgtg
    12061 ggtttgattc agatattact tgtggaaata ttacaaatga gacctataat gtttcagatg
    12121 aatgtattga gcgagaaata gaagtctatg ccaaaaaaga tgaaaggtta aatttagaaa
    12181 ttgttgtagg ctatagtaga gtatattctg atttaaatct aaatgaagaa cgtttaaaat
    12241 cattagaatg tctggagtcg ttaaaatgtt tagaatcaga agcacattta aaatcattag
    12301 aattggtttt aagagaagca tgtaatattg gatttgataa agtcaaaaat attcagagaa
    12361 agaaaatgaa tgagttttgg acaacctctg atgtagagat tgaaggcgat aaaactcttc
    12421 aactggggat aaaaaccaat ttatttcata tttatcaatc agcaggaaga gatggaaaga
    12481 ctaatatatc agcaaaaggt cttacaggtg atggctatga ggggcattat ttctgggata
    12541 cagaaatgta tatattacca ttttttatat atactcaacc atccattgct agagctttat
    12601 tagattatag atactcaatc ttagatgtat caagagaaag agctagagaa cttggaataa
    12661 ataaaggttg cttatattca tggagaacta taaatggtaa tgaatgcagt gcatattatc
    12721 cagcaggaac agcacagttt catataaatg cagatatagc ttatggtatt agaacgtatt
    12781 ttgaagcaac taatgatgaa gaatttatga tgcaaaatgg cttagaaatt cttatagaaa
    12841 cagcaagatt ttgggttgaa tttggagatt atatagaatc aaaaaataat aaattctgta
    12901 taaatggagt aacaggacca gatgaatata cagcaatagt aaacaataat tactacacta
    12961 atttaatggc taagtataat ttaatatatg cagtacagat ggtagataaa tttaagcata
    13021 atttattttc aaatgaagta tttagaaaag ttaaattaca agaatatgaa gtgaaaaact
    13081 ggataaatgc cgctcaaaat atgtatttac catatgatga agagaagaaa ataagcaaac
    13141 aagacgattc atttttagaa aaatcagtat gggattttaa aaatacacca aaagaaaaat
    13201 atccattact tctccattat catccattaa caatatatag atatcaagta aataaacaag
    13261 cagatactgt actttcagcg ttgcttttcc ctgatgagtt tagcttagaa caaaagaaga
    13321 gagattttga atactatgaa aaaataacta ctcatgattc atctttgtca aggtcaatat
    13381 ttggaatgat ggcaagtgaa attggaaatc atgaaaaagc ttataactat tttatggaca
    13441 cagcattaat ggatattaat aacctacaaa aaaatacaag tgatggaata catgctgcta
    13501 atatgggtgg aacatggatg agtttagtat atggatttgc aggaatgaaa ataaaaaatg
    13561 ataaattaag ttttgaacca aggataccta aacactggaa aaatattaaa tttaatatat
    13621 tatttagaga taatttagtt tctgtaaata tagaagaata taaatcttat tataagttaa
    13681 tcaaaggtag aaatataacc atattacatg ataataaaga aataaaatta tcaaagaaca
    13741 taatagaagt taataattgt tttaaaggga gtaattaata tgaaagcagt cttatttgat
    13801 cttgatggag ttataacaga tactgctaag tatcattttg aagcttggca tgagatagca
    13861 aaatcgctag atatagaaat agatgaaact tttaatgaaa aattaaaagg cataagcagg
    13921 gaagaatcat tagataaaat attgttatct gctaataaag tagatagtta tactaaagag
    13981 gaaaagttaa aggtgtgtga agaaaaaaat aacatttata taaagttaat aaactcatta
    14041 acatataaag atatattacc agggataaat gagcttataa aagaacttaa aaaaaataat
    14101 gtgaaattgg gaatagcatc tgctagtaag aatgcaccta ggatattaga atctttggga
    14161 ataaaagatg aatttgattg tatagtaaat ccatctttat taaaaagggg aaaaccatat
    14221 cctgatattt ttattgaagg gagcaagatg ctaaatttaa aacaatatga ttgtgtagga
    14281 atagaagatt cttattcggg tattaaagct ataaatgctg ccaatatgac atcaattggt
    14341 gtggggaaaa aagaagtact aggaaaatgt gaccatattg tagaagatac tagcttatta
    14401 aattacaata cactattaca agcttggagt aaaaagatta atttggccta aaactatatt
    14461 aaaaattatt ttaaaagaga ggataaggta tgaagaataa atatgaagat gtagctcaat
    14521 tgataattga tggacttgga ggaaaagata atataattga cttaactcat tgtatgacac
    14581 gattaaggtt catcttaaaa gatgaaaaga aagtcaatgc agataaatta aaatctatag
    14641 ataaggtagt tggagtaaac tcaacttcaa ctcaatatca ggtcataata ggtaatgagg
    14701 taggtgttgt atttaatgca ataataaata aggggataaa taccacagga gatattaaaa
    14761 gcgatataaa aaataaaaat aaaaaaaata gtattttaaa taatataata gatacaataa
    14821 ctggttgtat gacacctatg ataccagccc ttacagcatc tgggatgata aaagttatat
    14881 tatctctttg caccactttt aattggctat catcaaattc aagtacatat agagtattag
    14941 atataatagg agatgtagca ttttacttta tgcctataat acttgcaata aatgcagcta
    15001 aaaaatttaa tgttaatacg tcaatagcag ttatagtagt aggtgtgttt ttacatccaa
    15061 atttctctgc atgggtatca agtggggacc ctatatcatt tataggagta ccaattcaag
    15121 gtgttatata tgcagcatct attatccctg cacttttaac agtatggatg atgtcatata
    15181 tagaaaaatt cattgataaa ttaacaccta gcatgttaaa aacaatttta aatccaactt
    15241 tagtattatt aataagtgcg ccaatagcat taatagtaat tggaccaata ggaaatttct
    15301 taggagaggg attagctagt ataataaatt tactacaagg aaggttagga tttataatgg
    15361 tttgtttatt agcagctgca atgccattta tcgttatgac agggatgcat catgcattga
    15421 ctcctatatt catatcagca tttgcaacta caggacaaga atcgctaata ttaatatctc
    15481 aagtttgtgc taatttagcg caaggtggcg cgacattagc tgtagcatta agaagcaaag
    15541 atagtaagat gaaacagtta gcatcagctg catggatatc agctattatg ggtataactg
    15601 aaccagctct ttatggagtt acattgaaac ttaaaaaacc tgctatgtta gcatctatag
    15661 gagcaggaat cgcagggtta tttgcaggaa ttactcatgt gactttatat gttccacaaa
    15721 atagtataat ggcagtactt ggattatcag gggaaaaggg gatttcgaat atagtaaatg
    15781 gacttatagt tatatttata gcattggttg ctccatttat tatgacatat gtttttggag
    15841 ttgaaaaaaa tgttaaggaa ggtatagata atgtagtaga ggaagtataa tattaactat
    15901 ttacattaat agaattttta ttattattga ctataacttg ttttgtattt atattgatgc
    15961 aaagaaataa gttaatatta ataagaataa ttattagaac tgacaaaaag caacacaaaa
    16021 gattgatgaa gtatatggaa ataaaaagtt gacaaaatat gtctgatgtt ttatcatatt
    16081 aattggaatt aggtagtaat tgtttaagta caagctaagc ttattcatta ataatagcag
    16141 tatcctagga aggagtatgc ccattattat ggataagctt tttttatgct taaaaggagg
    16201 ataatcatga taattaataa gatattaaac aataatgttg taatcacatt agatgataat
    16261 gatgaagaag ttatcgttat gggtaaagga attggttatc aaaaatcaaa aggcaatcta
    16321 atagataaaa ctaaagtaaa taaagtattt agaatatcaa ataaagaaat atcaaacaaa
    16381 ctacaagaat tactcaataa tattcctata gaacacatga aattatcaag tgaaattata
    16441 gaatacgcac aaataaagtt aaacaagaag ctaaatgaaa gtatttacat atccttaagt
    16501 gaccatacat attctgcaat tcaaaggatg aaagaaggaa taaatgtaaa aaatgcaatc
    16561 ctttgggaaa ttaaaagatt ttataaagaa gagtttgaaa ttggaatgaa agcactagat
    16621 attattgaaa acaaaacagg tataaaatta ccagaagatg aggctggatt tatagccttt
    16681 catatagtga atgctcagtt aagtgaaggt catactttgg catctgatat tacgaagcta
    16741 atacaagaag tacttagcat agttaggtat cattttagga tagaatttca tgaagaatca
    16801 gtgttttatt atagatttat aatgcattta aagttttttg cgcaaagatt gcttttagat
    16861 agcgcacatg aaggtgagac agacaaagaa ttactaagca ttattaaatc taaatataat
    16921 aaagagtttg aatgtgtagt taaaattaaa aatttcatta aaaaacaata taactacata
    16981 ttaacagatg atgagcttat atatttaaca atacatttag ctaaagtagt taaagactca
    17041 aatatgtaag tgaaataatt tttcagcagt atattaagaa tgattgaaag gagtgatact
    17101 aacaaaatta tacttatggt tggatggtaa cattgagttg gccaaacctt caaatttcaa
    17161 aaaattaatt taaacgaaat atggaggaga aaaatgggaa aatacaaaca attagcacaa
    17221 gagatagtaa acaatgttgg tggaaaagaa aatattagtg gattagtaca ctgtattaca
    17281 agattacgtt ttactttaaa agatgaaagt atagcaaatg acaatgtatt aaaaaacatg
    17341 gaaggtattg ttactgtaat gaaaagtgga ggacaatatc aagtagttat aggaaatcat
    17401 gttgaagctg tctataaaga tgtagttgaa ataattggtt tagatgattc aagtacaagt
    17461 tcagaaacta aaaaatcagg taatatacta gataaaggta tagatataat atcaggaatt
    17521 tttcagccag tgttagggat aatggcagct tgtggtatgt tgaaaggatt taatgcatta
    17581 tttgtagcaa tgggattata tagtgatgta tctggtggtt atatggtaat aaatgcagcg
    17641 ggagatgcat tatttacatt ccttcctttg ttcttaggat atacttcagc taagaagttt
    17701 ggattaaaac caatgttggg attagcacta ggagctgcag tatgttatcc tgctatacaa
    17761 ggtagcagta tatcagcagg agcagatgtg atgtacactt tatttaaagg aacaatgttt
    17821 gcatcaccag tatatattga tttttttgga ctaccaattg tatcaattga ttatactgga
    17881 acagttgtac caattatatt tatagtgtat tttgcttcta ggtgtgaaaa attatttaat
    17941 aagtgtgttc cagatttagt taagtttttc tttgtgccaa tgcttacatt attaataaca
    18001 gtgcctgttg cacttatagt tataggtcct atagcaacat ttggttctac acttatttca
    18061 caaattgtaa tttcaataag agattttagt ccactattgg caggtgctat agtaggattt
    18121 acttggcaaa ttcttgttat ttttggtatg cactggggat ttattcctgt atatataaac
    18181 aatgttatga ctttaggata tgataatgtt atgatgccat tctttgcctg tacatttgca
    18241 agttcggcag ttgtgttggc aatatttttc aagactaaag ataaaaaact aaaagagatg
    18301 gcaataccaa actttatatc tggtatattt ggagttacag agccagcaat ttatggaata
    18361 ttattgcctt taaagaaacc atttataata agttgtattg ctagtggtat tggaggcgct
    18421 ttctatggat tctttaatct tagaaaattt ataactggag gaatgggaat atttgaactt
    18481 ccagcaatga ttgaaccaga tgggggaatg ggtaacttaa tagtagctct ttctggtata
    18541 gctatatcaa tggtcgtagc ttttgtatta actatgattt tatataaaga tgagaaagta
    18601 gaagagtcta aaaaagtaag aaataagggt aaagaagaaa taaaagaagt caagtctact
    18661 aaattagaaa gagaaatagt aacaagtcct ataaaaggtg aagtattaaa attaagtgat
    18721 atagaggatg cagcatttgc atctggtgta ctaggtcaag gggtggcaat aatacctagt
    18781 gatggaaaag tagttgctcc agtagatggg gtagttacaa ccttatttcc tagtctacat
    18841 gctataggga ttctttctga tacaggtgtt gaagtgctta ttcatattgg attaaatact
    18901 attcaattag agggaagagg ttttgaggca tgtataaaac aaggtgatag aattacaaaa
    18961 ggtcaaacta tattaaactt tgatgttgat gcaataaaag agttggggta tagtactgta
    19021 actccaattg ttattacaaa ttcatcacaa tttttagatg tagtagaaac agaaagtaaa
    19081 aatattgaat tagaagacaa tctaattaca gtcttatttt agtaaagatt gaggtattag
    19141 cataatctag tgctttggtt ataattttag gaggctttga ctatgagttt taagaatgat
    19201 tttttatggg gaggagctac tgcggccaat caatgtgaag gtggatacaa tgaagacgaa
    19261 agaggattgg caaatgtaga tgtatgccca acaggaaaag atagaacagc agttattaca
    19321 gggaaattaa aaatgtttga ttttgatgat gagcattatt atccagctaa aactggtatt
    19381 gatatgtatc ataattataa agaagatata aagttatttg ctgaaatggg atttaaagta
    19441 tatagaatga gcattgcatg gagtcgaata tttccaaaag gtgatgaaga aacaccaaat
    19501 gaaaagggac ttcaatttta tgaaaatata tttaaggagt gtaaaaaata taaaatagag
    19561 cctttagtca ctattacaca ctttgattgt ccaatgcatt tagttaaaaa gtatggtgca
    19621 tggcgtaatc gaaaaatgat aggtttctat gagaaattat gcaatgttat ctttagaaga
    19681 tataaaggat tagtgaagta ctggctaact ttcaatgaaa taaatatgat tttacatgct
    19741 ccatttatgg gtgctggtat ttgctttgaa gaaggtgaag atgttgaaaa gataaaatat
    19801 caagctgccc atcatgagct tgtagctagt gcaattgcaa ctaaaatagc acatgaagtt
    19861 gattcaaata acttaattgg atgtatgttt gcagctggaa gtgtatatcc ttatagttgt
    19921 aatcctaatg atgtatggga agctacaaag ttagatagag aaaattactt ctttgtggat
    19981 gtacaatcta aaggtaaata tcctaactat gcgttaaagt atatggagca aaaaggtatt
    20041 acaccagaaa tggaacctgg tgatattgaa ttattgaata aatatacagt agattttatt
    20101 tcattttcat attacaatag ccgttgtgtt agaaccgatg aaaatgcaga tgatatggct
    20161 gaaggaaaca tatttacatc tgctaaaaat ccatatcttt catacagtca atggggatgg
    20221 ccaattgacc cattaggttt gagaattaca ctaaatcatg tatatgatag atatgaaaag
    20281 ccactattta ttgtagaaaa tggattaggt gctaaagata tagctgatga aaatggttat
    20341 gtagaagatg attacagaat tgattatata agagaacata taaaagctat gcatgatgca
    20401 gtttctatag atggggtcga tttattaggt tatactacat gggcacctat tgatttagta
    20461 agtgctggta caggtgaaat ggaaaagcgt tatggattta tatatgtaga tagagacaat
    20521 agtggtaatg gaagtctaag aagaatgaaa aagaaaagtt ttgaatggta taagaaagta
    20581 attgcttcaa atggtgaaga tttatattag acaagaaatc tatgttaaat aagaaatttg
    20641 tattaaataa gaagagtata aaaattaata ataagtataa tttttaactt ttttaactat
    20701 agaaaatcaa tacagaatta ttttacattg tatttatgca atagtatgat tcacaaataa
    20761 aattttagaa aataaaattg ttaagaagta tgggaagtaa gtgcaattta ctttgtaata
    20821 aaagatgaaa aaggcaagct taggttttaa gcttgtcttt tttattatgt ataaactatg
    20881 atactttaaa attctatgaa aaatttattt aatagagaaa ttaaaaattt aatggtaatc
    20941 aaattcttta ccaatgaata taaaaaatgt ctacgtgtta taatagttta aactaataaa
    21001 cgaaaggtag atgaatgaat atgaaatacc aacatataat agaagttgac aaagaacttt
    21061 gcattggatg tgggctttgt aaaaatgatt gtccagtaaa caatataatt atagaaaata
    21121 aaaagtcagt aattaaaaaa caagattgtc taatgtgtgg acattgtgca gcaatatgtc
    21181 ccacaaaagc tattaccttg acagggtttg atgaacctcc tatagaactt acaaataagc
    21241 caaagctaga ttcagatgaa ttactaatgg caattaaatc tagaagaagt atacgtaaat
    21301 ttaaagacaa agaagtttct tcagaaatta taaagcaaat tattgaggca ggtaggtata
    21361 cccctagtgc taaaaattca caagatgtat catatattgt tttagacaat aaaaagtcaa
    21421 tttatgaaaa cgaagcagtg aaatttttta gaaaaataaa acctattgca aatatagcta
    21481 ttaaatattc aaaagaagtt gagattgacg ataacttttt ctttaagcat gctcctattg
    21541 caataatgat aattacaaaa gataagatta gtggttcact tgcagcatca aatatggcat
    21601 taatggcaga gtcttatggc ctaggtgttt tatttagtgg atatttttca gatgtagcaa
    21661 ataattcgcc aaaattaaaa aagcttttag gtctaaaacg cagtaatcat gttcttacaa
    21721 cattagtaat tggataccca gatgttaaat atagacgtac tgcacagaaa gaagtagcaa
    21781 ctgttagata cttataagag agggtggatt ttatggaaaa agaatgtcaa caaagaatgc
    21841 aagaaataac gtctaaattt aaagaatgtc gcaaagctat ctctgctatg ggtgatgaga
    21901 ctagacaatt gattttaatt gctctcttag agagtgattt taatggtata cgagtgggag
    21961 aaatcacaaa aaaaacacat ctttctcgtc ctgctgtatc tcatcatctt aagatattaa
    22021 aagaagcaga gattgttaat ttacgtagag aaggaacgaa aaattattat tatcttgatt
    22081 caaaaaaatc acaatggaag tcactaacag agctaataaa tcttatattt gttggaatac
    22141 aacatattag tgaaaatgat ataagaaaag gtgattaaat tatacttgat ttaatacaaa
    22201 aagattgatt taggaactta aaagttcact ttgagacact atcttttgaa ctatacccat
    22261 cttaggagac agttaaaata tgtaaaactg tataaaacaa tgggtatagt tcattttata
    22321 atggtttaaa tattaagaat atattattct aaatagtatc tttctctgtt ttgagtttta
    22381 tttttatact caatagcttc agtggggcaa ccacaaatac atgccataca atgagtacag
    22441 ttatttttcc aaactggttt tttgtttttt aaattaatat tatttaaagg acatagctct
    22501 acacattttc cacagccaat gcatttatca gtataatgaa atcctttggc actaataatt
    22561 gtcttataaa aaactgtatt tattatacca cttttaattg tacctcctaa gccactagga
    22621 gtaatagttt caaattcgtt ttcattgcgt atatcagaaa taatcctata tatttgttta
    22681 tctgcttctt taatcatttg ttttgatgtt tctttgtctg ttgtagaaaa taaagctata
    22741 taattctctg gcattttaat ttctgccata ccttttaatt gccattcctt atatttacat
    22801 agtttttgaa tatagtttat ggattttgct gtatctccac cacaagtcat tacaaagtaa
    22861 actctgttgc ttcctagaaa ttttgtttct tttataaaat ccgtaaccac tattggcaat
    22921 ctccatgcat aggtaggaca tacaaatatg aatggtttat ctgctgatat taattcatct
    22981 gttttttcct cttttataag ttgatttagt gatatcagtt catcatttaa ttcttgtgca
    23041 attttacgag ccacatatcg gctattacca gttccgctaa aatataaaat cataataata
    23101 tctccctaac tattttttat tttaactctt aattttttat ttattatagt aatttattga
    23161 tatattctat tcaaatgtat gtaaaattta gtagttaaga aaattataac acattagtaa
    23221 tttttatact tattggtaaa gaatttgatt accattaaat ttagcaacaa gacatcaact
    23281 ggacttgtat atgagaaggg ataataattt gcttatgaaa gattataaat ataaaggtta
    23341 ttttgaaagt gtagaaatat ttgataatgt aaatgacctt aacttaaata ctttaacaac
    23401 agttagtaga tttgtgcttg tttttttgtt tacgataact taaggaaata tgttacctga
    23461 ataataaatt tatataataa aagtatataa atatatatat gaatgttaag attagttgat
    23521 agaatttaaa taataaatcc aatattataa acataggagg tatgaaaaat atgattaatg
    23581 agaatttaaa aagtctaaga aaaatcaacc agtatacaca agaagaatta gctgaaaaac
    23641 ttaatgtttc tagacaatca atagcaaaat gggaaagtgg agaatctatt cctgatatat
    23701 gtagttgcat taaacttgta aaattgtaca atgtcaaact agatgactta gttaatcatt
    23761 cagaagaaaa aacaggtatt atagtacctc ctaaaggaaa gtttttcttt ggagcagttg
    23821 tagttggtga acgtggtcag attgtcattc caaaagaagc aagagaagta tttaatataa
    23881 atgcaggaga taaattattg gtacttggag atgaggaaag aggattagga attgttcatc
    23941 aacgtgattt aataaatttt ataggtcatg tgggagtagt acgtaaagaa gaatgatttt
    24001 aaaaaattat aaaaggaggt atgaaatatg agtaaaatag tattttttag cattcctgca
    24061 tatggtcata ctaatcctac tattgaagta gttcgtgaat tagtggatag aggaaatgag
    24121 gtattgtatt attcttttaa tgaatttaaa gataaaattg aaggtgctgg agcaaaattt
    24181 atttgttgtg ataaatacct tccagaatta ctccctggag atgagaaaaa aattggtaag
    24241 gattttcctg gattaattga gatgatagta gatactacta tttctttaga tgaaaaagtt
    24301 tgtagggaac ttaaagattt taatccagat tgcattgtat cagattcatt aagtttttgg
    24361 ggaaagttat ttgcacaaaa acttaatatt ccatatgtat gctctacaac aacttttgca
    24421 tttaataaac atacagcaaa gttgatgaaa caaaacttta tagaaattat acgtatgatt
    24481 tttggtgtaa gacgcattaa taaaaaaata aagcttttac aaagaaaagg gtatgaagta
    24541 aaaaatttta tttctatagt ttctaatgat aatgatacgg ataccattgt atatacatca
    24601 aaagaatttc aaccaatggt agaaaccttc tctagtaagt attcatttgt aggtccatca
    24661 gtatcaaagt tgattattga acctaaagag agaaaaagaa agctaatata tatttcgctt
    24721 ggtacaatta ataataaaaa tgttagcttt tataaaaact gtataaatgc atttaaagat
    24781 tctgatgttg atgtgattat gtctgttggt agaagtactg atattaaaag tttaggaaat
    24841 ataccaaata attttgaggt taaaaatagt gttgaacaga ttgctatact tcaaaaaact
    24901 gatgtgtttg ttactcattc tgggatgaat agtgttaatg aaagtttata ttatggagta
    24961 cctatggtat tatttcctca acattcggag cagagaatgg tagctaagag agttgttcat
    25021 ttgggagcag gaattatgtt aaaagaggat aaatcagaaa gtattaaaaa agcagtattt
    25081 caagttataa atgacaatga atataaagaa aatgcaacta aactatcaaa aagcttttat
    25141 aatgcaggag gctcaaaaaa agcagcagat gtaatattac agattatatg taatagtaga
    25201 caagttgaca tataatagat ttgttaaaaa tatatataca tacatgtaat ttaatcaaag
    25261 tactgacagt ggggtctttt aacctaccag ttggggcata tatattgatg ctaaatatga
    25321 gggatgttat attttaataa aaaagataac atcctttttt tagtgcatta tatcgagttt
    25381 taaataataa atgaaggagg attaatatga aaaaatataa agttattttt atagtattaa
    25441 ttataggtat tataagtgta acagggtttg ttgtatataa aaactgcatt aaaccatgga
    25501 aagatgctga aacatatatt gataagtata tggtaaaaca agatatttca aaagataata
    25561 taaaaagtat tacaaaagaa aaagccaaga aggcaagtta cgaaggaata ctatacaaag
    25621 tgtattacaa agatgaccct cgatatgagt atcaatattt ttatagtgat aattattatg
    25681 ctctatatgt aaataaagta atgttacaaa tttatgatat ggaaaataat aataaacttt
    25741 taagaagtaa tgaggaatta aaaagtgtaa agtatccacc aatatatctt aaatataagc
    25801 cagataaatg atttatgaca aaatagaatg att
//
LOCUS       DACXMZ010000032        25470 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.032, whole genome shotgun sequence.
ACCESSION   DACXMZ010000032 DACXMZ010000000
VERSION     DACXMZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.032"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            104..487
                     /locus_tag="JD016_05170"
     CDS             104..487
                     /locus_tag="JD016_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999023.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            995..1378
                     /locus_tag="JD016_05175"
     CDS             995..1378
                     /locus_tag="JD016_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAU4999024.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGLLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1380..1730
                     /locus_tag="JD016_05180"
     CDS             1380..1730
                     /locus_tag="JD016_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999025.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(1831..2289)
                     /locus_tag="JD016_05185"
     CDS             complement(1831..2289)
                     /locus_tag="JD016_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999026.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLEEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2334..3122)
                     /locus_tag="JD016_05190"
     CDS             complement(2334..3122)
                     /locus_tag="JD016_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAU4999027.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILNWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3261..3698)
                     /locus_tag="JD016_05195"
     CDS             complement(3261..3698)
                     /locus_tag="JD016_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HAU4999028.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            complement(3932..4066)
                     /locus_tag="JD016_05200"
     CDS             complement(3932..4066)
                     /locus_tag="JD016_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999029.1"
                     /translation="MGILEGAAMIREIAIKIAKEKGITEQKAWPEAVKEFKEKYELVL
                     "
     gene            4520..5029
                     /locus_tag="JD016_05205"
     CDS             4520..5029
                     /locus_tag="JD016_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999030.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEALETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            5056..5205
                     /locus_tag="JD016_05210"
                     /pseudo
     CDS             5056..5205
                     /locus_tag="JD016_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            5434..5640
                     /locus_tag="JD016_05215"
     CDS             5434..5640
                     /locus_tag="JD016_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HAU4999031.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTKLSLCYLTNRAQK"
     gene            complement(5941..6741)
                     /locus_tag="JD016_05220"
     CDS             complement(5941..6741)
                     /locus_tag="JD016_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4999032.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(6731..6919)
                     /locus_tag="JD016_05225"
     CDS             complement(6731..6919)
                     /locus_tag="JD016_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate/nitrate/taurine transporter
                     ATP-binding protein"
                     /protein_id="HAU4999033.1"
                     /translation="MAEAISISDKVAILSKRPATIKNIYDIELNMDGTKTPLKTRKVP
                     DFQKYFDILWKELDTHEA"
     gene            complement(6936..8534)
                     /locus_tag="JD016_05230"
     CDS             complement(6936..8534)
                     /locus_tag="JD016_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAU4999034.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KADKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLNN"
     gene            8856..9038
                     /locus_tag="JD016_05235"
     CDS             8856..9038
                     /locus_tag="JD016_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999035.1"
                     /translation="MHMEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNE
                     AFSILEKELNISKANT"
     gene            9212..10342
                     /locus_tag="JD016_05240"
     CDS             9212..10342
                     /locus_tag="JD016_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAU4999036.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            10583..10933
                     /locus_tag="JD016_05245"
     CDS             10583..10933
                     /locus_tag="JD016_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HAU4999037.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            10941..11390
                     /gene="eutP"
                     /locus_tag="JD016_05250"
     CDS             10941..11390
                     /gene="eutP"
                     /locus_tag="JD016_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HAU4999038.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            11492..12067
                     /locus_tag="JD016_05255"
     CDS             11492..12067
                     /locus_tag="JD016_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAU4999039.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            12060..13469
                     /locus_tag="JD016_05260"
     CDS             12060..13469
                     /locus_tag="JD016_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAU4999040.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            13653..15086
                     /gene="eutA"
                     /locus_tag="JD016_05265"
     CDS             13653..15086
                     /gene="eutA"
                     /locus_tag="JD016_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAU4999041.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            15104..16468
                     /locus_tag="JD016_05270"
     CDS             15104..16468
                     /locus_tag="JD016_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HAU4999042.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            16481..17362
                     /locus_tag="JD016_05275"
     CDS             16481..17362
                     /locus_tag="JD016_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAU4999043.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVEKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            17382..18035
                     /gene="eutL"
                     /locus_tag="JD016_05280"
     CDS             17382..18035
                     /gene="eutL"
                     /locus_tag="JD016_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAU4999044.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            18046..18747
                     /locus_tag="JD016_05285"
     CDS             18046..18747
                     /locus_tag="JD016_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HAU4999045.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKFANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDK"
     gene            18750..20219
                     /locus_tag="JD016_05290"
     CDS             18750..20219
                     /locus_tag="JD016_05290"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HAU4999046.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            20315..20602
                     /gene="eutM"
                     /locus_tag="JD016_05295"
     CDS             20315..20602
                     /gene="eutM"
                     /locus_tag="JD016_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HAU4999047.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            20729..21490
                     /locus_tag="JD016_05300"
     CDS             20729..21490
                     /locus_tag="JD016_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HAU4999048.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEIVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            21503..22132
                     /locus_tag="JD016_05305"
     CDS             21503..22132
                     /locus_tag="JD016_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HAU4999049.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            22174..22899
                     /locus_tag="JD016_05310"
     CDS             22174..22899
                     /locus_tag="JD016_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HAU4999050.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            22912..23184
                     /locus_tag="JD016_05315"
     CDS             22912..23184
                     /locus_tag="JD016_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutN"
                     /protein_id="HAU4999051.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            23177..23728
                     /locus_tag="JD016_05320"
     CDS             23177..23728
                     /locus_tag="JD016_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HAU4999052.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKICAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            23740..24831
                     /gene="eutH"
                     /locus_tag="JD016_05325"
     CDS             23740..24831
                     /gene="eutH"
                     /locus_tag="JD016_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HAU4999053.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            24833..25306
                     /locus_tag="JD016_05330"
     CDS             24833..25306
                     /locus_tag="JD016_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutQ"
                     /protein_id="HAU4999054.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
BASE COUNT        10057 a         3173 c         4219 g         8021 t
ORIGIN      
        1 atcttttctt tttggaataa actatacttt aaatatagat atttaattta aataaatttt
       61 acagtattaa tagtaaatta tcttagaaag gaatgtgctt tatatgtcta cagacaaagt
      121 tgttggaatc atagatgaag aaacggcaga acttgcagga attgaatata ctggtaaaat
      181 ttatgcttct tctggtgtta taaaacatat aaagaaaaaa catagatgtc aactgagtaa
      241 ggatattttt aatgatataa tagacactat aaaaatggta ttaaagtcac ctgaatatat
      301 aggaagtcat cctaaaaaac ctggaaaaag tgtggagttt ataaaaaaaa ttgatgatta
      361 tattttagta gctacagaat tagatacaaa aaaaggatat ttatatgtat cttcaatgta
      421 tgctataaaa gaagcaaaat tagaaagtag agtaaacagt ggaagagtaa aacaatatga
      481 tggttaacat aatattatta attttcccta aaatattgaa aaatattatt atataagtta
      541 taatattaat atacacgtag atatttatta gttagatttg aggttggaaa aggcacccaa
      601 tacgctacta tagagtagtt aatagagatg caggatacgc cgccctgcca aatctaatta
      661 aattaaaaag agaggattaa ttcctctctt attgtttttt tgagatacta atttaatcta
      721 taatagaaag attgaaggtc gtttatacaa aaaaattttc agttatatac attaaactaa
      781 ttaaataaga tatttataat ctatatgtga tacatattat atattaataa aagaaaaata
      841 tatagaaaat ccttataaaa atttaaattg taatgaatag gaagtaatta aaaagaattt
      901 ttattgattt ttaaaaattt agttagaaac tatatcatta tggtaaaaaa tgtattataa
      961 tgtttagaaa tattataata aggagcgatt aaacatgaat ctatcgaaga aaatagaaga
     1021 taaaagaaaa gctaaacaga gagctgaaaa agttaagaaa gctaaaattg ctacagctgg
     1081 tgtggtatta ggagctgtta ctggtgcagt gagtggatta cttttggctc ctaaatctgg
     1141 aaaagaaaca agagaggata taaaagatgc ttcacaacaa attgctgaaa agataaatat
     1201 gaaaactgta gatgtaaaag gcaaagtatc tgagaagcta gaggataaaa aaggaaactt
     1261 tatagaatca aagaaaaaaa taaaaaaata tatagataac aaaaagagtg ttgaaaattc
     1321 tagtgaggaa gataaaataa tagatatagt tgaaccagtt agtgttactg aggaataata
     1381 tggacaatac aataacgatt aacataagta taatatatgt attattagct atacttgctt
     1441 gtattgcttt agtttattta attattgtac ttgttaaact acataagatt ttaaataatg
     1501 ttggaactat gttaaatgaa aatactcaaa atataaatac aactttaaat aatcttccta
     1561 atgttgtaga aacttttgga gaagtaggag aaaatatgaa agatgtaaca gaagtagtta
     1621 ctgaagttac agcagatttt cttgttacta aagacagtgt caaatcaaat atggatgtta
     1681 tagttgatat attgactata attaaaaaca tttttctaga taaaaaataa taagttaata
     1741 taaaataagc ctataagata taatttaaac gaccttaaaa tagcatttat aaggtcgttt
     1801 tttattagct tgatttgaca ctatgtgttt ttaataattg gtaatattcc tctttatcat
     1861 ttgaacatat atatctatgc ttgttgtttc aattacatcc ataagaacat ctttacattc
     1921 aagctcaaaa tcttccattc cttctaaata ttcatcatct tcatcaaaaa agatataaga
     1981 attatatagc cattcttcaa atttttcttc aagtatatcc caatacgaac tccaaaataa
     2041 tccacaacct gatatataag atgctctact ttgatttata taaacctcct ctagaagctt
     2101 ttgtaatcta ctgctataaa gaaaatctgc tcttccactg cttgatatta attcatttaa
     2161 atcttctatt gacattaatt caactttacc acttttatca taaggagtat aattttcatc
     2221 aacccattta tgtggtttgt tttctctgca tactgttgct atcaactcta caacttttgt
     2281 tcttatcatt tttttatcct aacttattta ttttttataa aataatcatg attttataat
     2341 ttatctattt cttgtaatgc taatttaaca gtttcatatc tatcagcacc atttattact
     2401 acatcacctt ttacaagttt attagcttct ccacctacaa cataaacctt tttacctaaa
     2461 ccttgcttat aactagacgc ctcaattatt aaagaatctt tcaattgcca atttaaaatc
     2521 tgagcagcca ctttatcagc atcacctgta taaacgatta cattttcata cactttattt
     2581 tcccctcccc cttgagaatt tgatatagat ttgtttagta taccttctgc tatcaattta
     2641 gcaactttat ctttattatt aacataataa tctgtatctg ctttactatc tacaaaacat
     2701 acttctatta atattgctgg tgcaatagta tgattaagcc aataaagacc tctaacatca
     2761 gactttgcac ccctatcttt gaatactgta gctaacctag tattcactct ttctgcatag
     2821 gttttgccat tattagtctt atatattgtt tctgtcccta ctggagttga agttgtagca
     2881 tttgaattaa aatgaatctg aacagccaaa tcagtttcct gtttatttgc tatagcacat
     2941 tggtctgcta aatgattact tgattcatca actcttcctg tataaacagt agctccacct
     3001 attttaagcc atttaacaat taagtcattc agtattcttg tttctttgct ttcgtttata
     3061 tacccaacag ctcctgtacc ttttccaatt aaagtgtgcc ctggtattat tactactttc
     3121 aaactaacat ctcctttaca attaaattta acttctattt aatatatgca taacctagaa
     3181 ttttgtttct ttttatatct tatactttta ttataaccca ttttgtaaaa atatagaata
     3241 ttaagtaaat aatgttaaaa ttataatttt gtatataaga cattacttga ttgcaataat
     3301 tttatagcat attcatctat tctatatcca gttctataat atatatgttt aattccagct
     3361 tgaattatag ccttagtaca atttaagcaa ggaaagtgag ttacataaat agagcaatct
     3421 ttaactgaaa taccttcttt tgcacaataa taaagggcat tttgttcagc gtgaatggtt
     3481 ctaatacaat gtccatctct catttcatga ccaacttcac tacaatgctt atctccagaa
     3541 atactaccat tatatccagt agacactatt ctgttttctg aattgacaat tatagctcct
     3601 acataagctc tatcacaagt tcctctttca gctactgttt cgcacagtct cataaaatat
     3661 tcttgccatg agcatctatt ttcaagtttt ttattcaaat catatacctc caaaatttat
     3721 atctaatata taagatttat accttaaaaa taatattaaa aacaaattta tacgataaat
     3781 aaaatttatt aatactgttt ttagtctaaa ttatttcatt ttaaattaaa gttacaagga
     3841 atctaagatt atatatatct ttcttcgtaa tatgaatgtt acaatataaa aaactgtctc
     3901 aataaaatag agacagtttt acagtcaaag tttataatac aagttcatat ttttctttaa
     3961 attcttttac agcttcgggc catgcctttt gttctgtaat acctttttct ttagctattt
     4021 taattgcaat ctctcttatc attgcagcgc cttctaatat tcccattttt aatctccctt
     4081 caaatatctt tttgtataga atataaatag tgctaagatt tattacatgc tgcatataca
     4141 atatctatac aacacataac ttgtaaattc cttttatttt catacgtctt taaccgacat
     4201 ataattatat tcaataagtt ttaaaattat gaactcaatt ataattattt ttattttttt
     4261 agaatctaaa cactaaaaat tgtaggtaaa tttgtcttat gcctttataa atatttctac
     4321 agttttaatt agtaaacata tctattttta ttataaaaag cgattaaaaa tggagaatat
     4381 cctcataaaa actatatata agcaaaacgg ttaaaggaaa aagaagaaat ttacttgaat
     4441 gttaaaatgc gttgcttgaa gcaatgggca gttttgccta taatatcggt aacaactgaa
     4501 agaaaggtgg ttagccctaa tgttaagcga aaacattaaa acaatcagga agtcaaaagg
     4561 actttcacaa caagaacttg ctatcaaact gaatgtagtg cgacaaacaa tatctaaatg
     4621 ggaacaagga ctgtcggttc ccgattctga tatgttgatt tccatatcag aggcgcttga
     4681 aacgcccgta agcactttac ttggagaaac tattgttgag tcggaagttg atagcttaaa
     4741 agtgatttct gaaaaattag agattataaa cttgcaactt gcacaaagaa aaaacatgag
     4801 aagaaaaatg tttcattggt tatttatttt attatgtgca gtcatattaa tagtttttgt
     4861 ggtcttaggg ggactaaata gtccttactt ggattggaat tataccgacc ctgaaactgc
     4921 tgtttttgga gtagcttttc atgcttttga gtggctattt gtcagattag taccgattgt
     4981 ccttatagga gcaattattg gaatcttttt gacacgtaag aaaatatgaa gtaactctgc
     5041 tttttataat acagcaagcg ttccttaaaa gtgtcattct acatggcaat cgaggacaac
     5101 tatgtaagga agaacctttt tgatttccaa ctaagcgaag ttattgagga cgacagtagg
     5161 ctcaaaaccg cattaagtaa agtacaggaa gcaagactgc tggacttata ctctcatgcg
     5221 tcagatgcag gaaatgcgag gtctggtagc atgatttaca acgatttgta caacgactac
     5281 gagtagaaat atatggagat aagtattatc tacgaaatca gaaaagaaat gacttgataa
     5341 tttacccatg ttataattat tgatgtgaaa ttaataaatc gaaattcctt ttttagattt
     5401 aacaatactg agtaaggaaa aagtgaatga cttttgcaag gaggtgttaa tatcaggcaa
     5461 gtaaagtctt ttgtttttcc agttatatcc ttaatattct tagaccaaat tagcaaagtt
     5521 cttataggat tattcttaat ggactttgaa attgatataa ttgggaaatt tttaagattc
     5581 aatcctgttc aaaatacaaa attaagtctc tgctatctaa caaacagagc acaaaaataa
     5641 aaattaaata ttaaatataa agcacagtta attttgatta aaaagttgac tgtcttttct
     5701 tttgtagaaa tttaagcata ggtaataaag aaatgactgc atttaagcat tatgtatata
     5761 attggaggca ataaaaagcc agcaaagccc ataaaataga gctgtgctag caatatgatg
     5821 agttataaga agataatcaa aaacttacgc tttattttaa tttaaaaaaa tgattactgt
     5881 aaagaaatca ttttttatta taatcactga tacatcaaat attctaaata aaatttttat
     5941 ttatctggaa ttgtatattt tttctataat attaactaca gcgtacattg cccaagcaca
     6001 gatagccaat acaaatactc ccatcattac taaatctaat ttaaatactt gagaaccata
     6061 tactatcaag tagcctattc catatcttga aactaaaaat tctccaacta ttacacctac
     6121 ccatgccata ccaatattaa ttttagttaa atttattaaa tttcctgtgt ttgcaggtaa
     6181 tatgagttta aataatattt gagatttagt agctccaaag ctttttaaca tcttaatttt
     6241 ttcttcatct atattcataa aataattata agctgaaagt atagtaacaa caacagaaat
     6301 cgttacagct gttacgacga ttccttctat tccagcacct acccaaacta taattatagg
     6361 agctaaagca gtcttaggaa gagcatttaa aactaccaaa aatgggtcta atatttttga
     6421 taatttttct gaccaccaaa gagcaattgc taccaaaata cctaatacag tacctataac
     6481 aagaccaaga acagtttcat atacagatat tccaatatgt ttaaataatt gaccactaaa
     6541 tgcatattga ataaataaat tgtaaatatc acttggtttg ctaaatagaa atgtatttat
     6601 aacatttaag tcagctaaaa gttcccagat tacaataaaa ccaattaaaa ttaatagctg
     6661 gtagaataat acatttcttt tttctctttt tacaccttta acatagttta tatatccttc
     6721 agatctttta ttaagcctca tgtgtatcta gctccttcca taatatgtcg aaatactttt
     6781 gaaaatcagg aactttacgt gttttgagag gcgtttttgt tccatccatg ttaagttcaa
     6841 tatcgtaaat attttttatg gtagctggtc ttttagatag tattgcaacc ttatctgata
     6901 tgcttattgc ttctgctata gctaaaaaag tgcaactaat tgtttaaaga aacagaatta
     6961 tccacttcag cacccatata aacaatatta agatttccta aactgctatc ccaaacgata
     7021 ctattaataa tagtagataa taacaatctc ttatcatcta aactggcatt atcaacttca
     7081 tcattaaatc tcttcaaatt actaacaata atatcaatat taatactgtc actactaata
     7141 ttgatgttac tagattccaa agtattaatc ttatccttta aaatactaat ctcattattt
     7201 aggctctcaa tctgggaaac tatataatca gaagcagtac taccagtatt ttttgcaagc
     7261 tctataacta aatttttaat aagtctttcc ttatctaaaa tttgattttt aagtaaatta
     7321 gtctcagatt taaaaatatt aaaaacatta tcagtcttaa tgttattact attgaatgca
     7381 cttataatag actcttcttt acaattctta attttatcta taactaaact ttcaactaat
     7441 ggaccattaa gatttttaca gttgcaacta tcagacccaa gtgttttttt agtaccacaa
     7501 atataataat actttaaatc cttgtcagct tttttgcctt tataggtaat tctcatatta
     7561 gaaccacatt taccacattt tataagacca gataaaagag cagtttttcc agttgatatc
     7621 cttggagttt tatcacgatt tttatctaat tgattttgta cttcaagcca tatttcaggt
     7681 tctatgactc catggtgttt tgcaattgct gctattgagt tagatgtatt tttagcatag
     7741 gtaaggattc cacattttct atctggagta cctacaacat ccataccact atttttaagg
     7801 taatctaaaa tttcatcatt agcttttaca tatgctggat ttctaagtat tctagataaa
     7861 gaactagggt caaatttacc accacgagga ccccttattc cattattata gacatattta
     7921 tataacttag ataaggacct aagttctaaa tatttattgt atattagctt tacaatctcc
     7981 atttgttcat catttacttg aagcttatac atacttcttt cttttagatt ttcatctaag
     8041 aatgtgattt tttcactgtt gaatccatga ggtgtattgc ctccaagcca tctaccacta
     8101 cgggctaatt catacatatt gtcttttata cgttctgcta ttgtttctct ttctaattga
     8161 gcaaatacac ttgatataaa catcatagct cttcccattg gagttgaagt atcaaattgt
     8221 tctttgatag atataaatga tatgtttagt tcatttaatt tttctattaa agtagagaag
     8281 tcagatacat ttctcgatat cctatctaat ctataacaaa ctaggtaact aaatttctta
     8341 tctttggcat cctgcatcat tttcttaaat tgtggtctat ctatattgct accactaaat
     8401 ccctcatctt catatatcaa aaaatctttt acaccaatag actttgcata atcaatacat
     8461 aattgtattt gattttctat agaatcacct tttccagtaa atcttgattt tcttgaataa
     8521 attgcagctt tcaaaattaa aacctcctat aagttaggat atatatttga aactatttat
     8581 ctacaacctt ttaagtaaac ttattagaat gaatttatta attaggtaaa tttatataat
     8641 tctacttttt ataatatatg actatttatt ttatttcttt actattcaag ttctaaatca
     8701 ataataaatt ttgatattta attgtatcag gacaaatctc taatttcata gattattaat
     8761 ataaaatcat aggttagtgt gaaaaagttg aaatttaaat attttaaaag ataatttttt
     8821 tgaactaata ggtataacaa aaatttatag ggggattgca tatggaaatc aatgttatag
     8881 aaattttccc taaagataaa gctaaactta ataaaataga aatggataaa gctagttggt
     8941 ttgtaaatat aataagtaaa aaatatccta aagaagcttt aaatgaagca tttagtattt
     9001 tagaaaaaga attaaatata agtaaagcta atacataagc tttgattatt gattttatat
     9061 actattggtt ttatacgcta tgacttgaca ttatattaat caaatagtat atttttaaat
     9121 atagcacaat gaagtgttaa aagagcaatg gagctccata gataaagtct atggagcttt
     9181 tttgcgtatt taacttaagg aggataggat tttgaataac tttgagatta aaactagaat
     9241 aaaatttgga gaaggctcac taaaagcttt gcaagaatta aaaaacaaaa atgtactaat
     9301 aataacagac ccttttatgg tgaaatcaaa aacaatagat aaaataacat ctaatttaat
     9361 aaactcaaca tatcaagttt ttgaagaaat agtaccagac ccaccaatag aagtagtagt
     9421 agcaggaata gaagcattta aatctataaa tccagacgta ataatagcat taggtggtgg
     9481 ttctgcaata gatgcagcaa aatctataat ggacttttct aaaaaaatat taaaaattaa
     9541 cgaagtagag tttatagcaa taccaacaac aagtggaaca ggttcagaag taacatcatt
     9601 tgctgtaata acagatagtc aaaaaaaatg taagtatcca ttagtgagtg atgatttact
     9661 tccagatatg gcaatactag acccagagct tgtaaaaact gtaccaaatt ttataacagc
     9721 agatacaggg atggatgtat tgacacatgc tatagaagct tacgtatcta caaattctaa
     9781 tgacttttca gatgcactag ctgaaaaagc tataaagtta gtatttaaat acttaataaa
     9841 agcatataaa gatggaaatg acatagaagc tcgagaaaaa atgcacaatg catcttgttt
     9901 agcaggtatg gcatttaacc aagcatcact tggcataaat cattctattg cacatgtatt
     9961 aggtggaaaa ttccatatac cacatggaag aacaaactct atattacttc ctcatgtaat
    10021 agaatataat tcatcaatag taggatatac taacacaact tactctgata cagctaaaaa
    10081 atatgcacaa atagcagaat tagtaggact tgggagtgct aatataagaa tatctgttag
    10141 aaatttagta aatgaaataa agaaactaca aaaagaaatg aatatgccaa ctaaattgac
    10201 acaatgcaaa ttaagtttag aagatataac taatgaagaa agagaaatag caaaacttgc
    10261 tataaaagat acttgtacat taactaatcc taaggtacca actgaagatg atataataaa
    10321 catattaaag aacattaaat aactacattt tattacatgt aaatataata ttatacaaat
    10381 agggaaaatg tttttaaaaa tataaattat atattgacaa atataaaaca taaaactata
    10441 atgaaagtat cacaaaggcg tgatacttta gtagaaagca atggagcttc ttaggaatat
    10501 taatattatt cttatggagc tccttttatt ttaatcaaat agctttttaa taaactatat
    10561 tcaaagaaag cagggaataa atttgactga agaatctaag caaagagtta ttcaagaata
    10621 tgttcctgga aaacaggtta cattagcaca tatcatagct aaccctaatg aagatatata
    10681 taagaagtta ggattagtac ttgataaaaa agatgcaata ggaatactta caataacacc
    10741 aagtgaagcg tcaataatag cagcagatgt tgctacaaag gcttcaaatg tatctttagg
    10801 ttttatagat agatttagtg gttcagtagt aatatcaggt gatgtaagtt ctgtagaatc
    10861 agcgttaaat gatgttttag aagtcttagg aaatatgtta aacttctcat ctacaaaaat
    10921 cacaaggaca taataataca atgaagaata taatatttat gggaaagaca ggtagtggta
    10981 aaactacctt atgtcaaaaa ctagaccagt tagaattaaa gtataaaaaa actcaatcag
    11041 tggaacttta taataatgca atagacactc ctggtgagta tatggaaaat agaaacctat
    11101 ataatgcctt aataacaaca tcagcagatg ctaaaattat agcaatagtg tatgatgcca
    11161 cacaagaaga aaattatata gcaccaggtt ttgccagtat gttttgcaaa gacgtaatag
    11221 gaattataac taaaataaat aaagctactg aaaacgaagt caatatagct tatgaaagac
    11281 tatcactagc aggagcaagt caaatcttta aaatagacac agttgataat attggacttg
    11341 aaaaattatt taaatatcta gaaaaatata aaaatataga tgaagtttaa ggggaaatat
    11401 ttttttattc aaaaatatta ttaaagtatg ataaaataaa aaaatgtata aactatttta
    11461 agaagattta atctttaagc gaggtaggaa catggggaaa aaaatactat tggttgatga
    11521 tgaaccactg ttaaggatgg atacaaaaga tatattagaa agtcatggct atgaaataat
    11581 aggcgaagca agtgatggat ttgaagctgt tgaaatgtgt aaaaaacaca aacctgattt
    11641 agttataatg gatatagaga tgccaatact tgatggtata aaggcaggca aaataatatg
    11701 taaagaaaat ctagctggtg gagtactttt attaacttca tttgaagata aagaatacgt
    11761 agaaaaggct aaaagtatag gtgcttatgg atatttagta aaaacagcaa gtgaaaaatc
    11821 acttatccca accatagaaa tgtgtttaaa taaggtaaat gaatttgaaa aaatgaaaaa
    11881 agatttagaa aaagtaaatt ctaaattgaa tgaaagaaaa ttagtagaaa gagcaaaagg
    11941 tatacttata agagagttta agatttctga ggatgaagca tacactagaa taagaaaatt
    12001 aagtatggat aaaagaacta caatgatgga aattgccaaa atgattataa taggatacga
    12061 tgactaatac gataaaaaaa atatcaactg cacaaacata cttaaataat aacgaaattg
    12121 aaaaaattat agaagtatca aaatccttag atttaatggc ttctttttat gaagctgatg
    12181 tttttataga tgtactaagt aaatttgatg atgaagcaat tgttgtggca catggtagac
    12241 ctaaagaaaa gtcaatgtac tgtgaaaatg ttataggtaa aaaggcttta attgaaaatg
    12301 aaccaggagt tataaagtgc ttaaaaacag gagaaagagt aagagatatt aaagctttaa
    12361 cacaggaata taaactggtc aaacaaaata ttcagccaat aacactagat gacaaagttt
    12421 tagcagtact aatagttgaa aaagatataa gtagtgaagt taagaatgag tttaatgcta
    12481 gtgaagacga agaaaataaa aatttacatc agtttataaa acttatcgga aataatgact
    12541 ttttagttga taatttaaat tcagccatat taatatttga taaaaatgga aaattaaaaa
    12601 ttaaaaatcg tattgcaact gagatataca aaaatcttgg gtttggatat gatatacaag
    12661 atatgcatta caatgattta gatatagaaa ccaacacctt tgaaaatata ttaatggatg
    12721 aaaattatga tgagacaaat gaagtttcaa taggtaatat gttttttaaa ataaagacca
    12781 tatacatcaa agatgatgaa tttaaggttt gcaaaatagt acaagatata agtgatttaa
    12841 agagaaaaga agaagaatta gttttgaaaa cagtggctat aagagaagct catcatagag
    12901 taaaaaataa tcttcataca gtaatatcta taataaaaaa acaaagtaga ttatctcaaa
    12961 atgatgaagt aaagcagtgt ttagataaca taataaatag ggtatttgca atactttcga
    13021 ctcattattt actatctaaa gaagttgata ataatatatc tatacgagaa ggtatcaatt
    13081 tattagtgtc aaatattcaa ggtggctatt tatttagtaa ggatatagat atacgtataa
    13141 ctggtgaaga ttttaaaatt agtggagaaa aagcaacagc aatacttttg gttataaatg
    13201 aaatattaca aaattgttat gaccatgcat tttcggatag agaacatggt actatacaaa
    13261 tattaataaa taaagataac gactatagaa atattgctat tatagatgat ggaattggat
    13321 ttgatagtaa taatataaat aaaaatagct tagggacata tataatagat agttatataa
    13381 cacagatgtt aaaaggaaat atagagagaa aaagtagtga gaatggtaca gaagtattaa
    13441 ttactatacc tacacaaatt aatatttaaa taataactca tctacaaagt agtagtttta
    13501 attgagcaaa gaggcttaag gttattaata gattaagtta attgaatgta tttataaaaa
    13561 ttataagtat atattcaatt taacaggtat ttattataac tttaagcctc tttgcttgtt
    13621 taagacatac ttaaatttta ggaggtgaga aaatgagaga agagttatta agtgttggta
    13681 ttgacattgg aacaagtact actcagttaa ttttttctaa attaatagta gaaaatactg
    13741 aatcaaattt ttcggtacct agaataagta tagtagacaa agaaattatt tataaaagca
    13801 aagtttattt cacccctctt atttcaaata atgaaataaa tggtcaacaa ataaaagata
    13861 tagttgaaaa tgaatataaa aaagcaaata tatctaaaga agatatacat acaggagcag
    13921 taataataac tggtgaaaca gctagaaaag aaaatgcaaa tgatgtactt catactttga
    13981 gtggatttgc tggggacttc gttgttgcaa cagcaggacc tgatttagaa agtataatat
    14041 caggaaaagg agctggagct catatttatt caaaagaaca ttcaactaca attgtaaata
    14101 tagacatagg tgggggaact agtaacatag cctcatttaa aaggggagaa gtaaccgata
    14161 ctgggtgttt ggatataggt ggaaggcttg taaagataga taaaataagt aaaaaaataa
    14221 cttatatatc acctaaaata gataaaatca taaaagataa taatttaaat cttcaagtag
    14281 gtacaatggc tactgtagaa aatacaaagc caatagtgga tataatggtt gacttattaa
    14341 tggaaagcat aggattaaag aaacagacac ctttgtttga ttttataata accaataaat
    14401 caataagtat agatgaaaat attaaaaatg tttcattctc tggtggagta gctgattata
    14461 tttactatga tggagaaata aaagattatt ttgaatatgg tgacttggga attttacttg
    14521 ggcaagcaat aaaaaattgt gaatacctta aaaaagtaaa tgtagttaaa tctatggaaa
    14581 caataagagc cacagtggtt ggagcaggtt ctcatactac tgaagtaagt ggaagtacaa
    14641 taacttacac tagcgaaaag tttccactta agaacttgcc agttttaaaa ttatcaattg
    14701 atgaagaaac aggcacttca aaacaaatca gtgaagctat aaaaaataaa cttaattggt
    14761 ttaaactaga aaatgattta cagaaagttg ctatagccat gataggaaag aaaaatccaa
    14821 cctttttaga tatacaagag tatgctaaag ggtttgtaga tggaatgcaa caaatcatag
    14881 aaaatgattt accttttata gtaattgttg aaaatgatat ggcaaaagtg ttaggtcaag
    14941 caatatatgc attattaaat tatgaaaaag aaattgtttg tatagacagt gtcaaggttg
    15001 aaaatggaga ctatatcgac attggaaaac ctatagttga aggtaaagtt ttacctgtgg
    15061 taataaaaac attagttttt aattaataga aggaggtaag taaatgattt taaatacaaa
    15121 attatttgga cgtacttatc agtttaaagg cttatatgat gtaatggcta aagctaatga
    15181 agaaaaatca ggagataaat tagcaggtct tgcagcagaa tcagctgaag aaagaatagc
    15241 agcaaaagtt gttttatcac acataaaatt ggaggatata agaaataatc ctgcagtggc
    15301 atatgaagaa gatgaagtaa caagaataat acaagatgct gtaaatgaaa aagtttatga
    15361 agaaataaaa aattggacag tctctgaact tagagaatgg ctattggatt caaatactac
    15421 aagtcttgat ataagaagaa tatcaagagg attaacttct gaaatgatag cagcagttgc
    15481 aaaattaatg tcaaatatgg acttagttta tgcagctaaa aagataaagg ttactgctca
    15541 ctgtaataca actataggag aacaaggaac attatcagca agacttcaac ctaatcatcc
    15601 aacagacgac ccagatggaa tacttgcatc acttcttgaa ggtttaactt ttggtatagg
    15661 agatgcagta cttggattaa atccagttga tgactctgtt gagagtgtta ctaaagtatt
    15721 aaaaagattt gaagaagtaa aacaaaaata tcaaatacca actcaaacat gtgtattggc
    15781 acacgttact acacaaatgg aagcaataag aaatggtgct cctgcagatt taatattcca
    15841 atctatagca ggttctgaaa aaggaaatga agcatttgga tttaacggcg acactataga
    15901 agaagctaga cagttagctt taaaacacgg tacagctaca ggacctaacg ttatgtattt
    15961 tgaaactgga caaggttcag aattatcttc agaagcacat catggaatag accaagttac
    16021 tatggaagct agatgctatg gatttgctaa aaagtatgaa ccattcttag taaatacagt
    16081 tgttgggttt ataggacctg agtacttata tgatgctaga gaagttgtaa gagcaggact
    16141 tgaagaccac tttatgggta aactacatgg cttaccaatg ggtgttgatg tttgttacac
    16201 taaccatatg aaggctgacc aaaatgatat ggaaaactta gccatactat taacaacagc
    16261 aggatgtacg tattttatgg gaatacctca tggagatgat gttatgctta actatcaaac
    16321 aacaggatac catgaaacag catcacttag agaaatgttt ggactaacag caataaaaga
    16381 atttgatgct tggatggaaa agatgggatt ctctaaagat ggtaagttaa cagagttggc
    16441 gggagacgca tctgtattat taggatagga ggtatttaat atgaatgaga aggatttaaa
    16501 agcattggta gaacaattag ttggacaaat ggttggagaa ttagatacta atgttgtatc
    16561 tgagactgtt gaaaaggcaa ctgaagttgt agtagacaac aatgcttgta tagatgatat
    16621 aacagaagtt gatataagaa agcaattact agtaaaaaat cctaaggatg cagaagctta
    16681 tttagatatg aaagcaaaaa cacctgcaag attaggtata ggaagagctg gtactagata
    16741 taaaacagaa acagttttaa gatttagagc agaccatgct gctgcacaag atgctgtatt
    16801 ctcttatgta gatgaagaat ttataaaaga aaataatatg tttgcagtgg aaactttatg
    16861 taaagataaa gatgaatact taacaagacc agatttggga agaaaatttt ctccagaaac
    16921 tataaataat ataaaatcta aatttggtac aaatcaaaag gttttaatat tagttggaga
    16981 tgggcttagc tcagctgcaa tagaggcaaa tttaaaagac tgtgtgccag ctataaaaca
    17041 gggtctaaaa atgtatggta tagattcaag tgaaatttta tttgttaaac attgtagagt
    17101 tggtgctatg gaccacttag gtgaagaatt aggttgtgaa gttatatgta tgttggttgg
    17161 agaaagacca ggattagtta ctgctgaatc aatgtcagca tatatagctt ataagccata
    17221 tataggaatg gcagaagcaa aaagaacagt tatatcaaat attcacaaag gtggaacaac
    17281 tgcagttgaa gcaggtgctc atatagctga acttataaaa actatgttag ataaaaaagc
    17341 ttcaggaata gaccttaaat aaagtcgagg gagtgtagaa tatgaaaaat gatgtaattc
    17401 gtcctaatat actaggaatt aaaataattt caaatataag tccagaaatg gcacaaaaac
    17461 tagaattaaa atcacatcac aaaagcttag gtttaataac tgcagactgt gatgatgtta
    17521 cgtatacagc tcttgacgaa gcaactaaag cttcagaagt tgatgttgta tatgcaaaat
    17581 caatgtatgc aggtgctgca aatgcttcaa caaaacttgc tggtgaagta ttaggaataa
    17641 tagcaggtcc aagcccagca gaagtaagaa gtggtctaaa tgcagtagtt gattttctag
    17701 agtatggagc aacttttata agtgcaaatg atgatgattc aatagcatac tatgctcact
    17761 gcgtatcaag aacaggtaca tacctttcag aggtagcagg aataagagaa ggtgaagcat
    17821 tagcttattt agtagcacca ccacttgaag caatgtatgc actagatgca gcaatgaaag
    17881 ctgcagatgt taagatgtgt gaattatttg cacctccaac tgaaacaaac tttggtggag
    17941 cattacttac aggttctcaa tcggcttgta aagcagcatg tgatgcattt gcagaagcag
    18001 taaaatcagt tgcagacaat ccaacaggat tttaaggata gttagatgga tgctataggc
    18061 ttaatagaag taataggata tgttgctgca atagaagctt ctgatgcttg tttgaaattt
    18121 gcaaatgtta atattgtaag agttgataag gttggggcag gtattgttac tttaacaata
    18181 tgtggagatg tcggtgctgt aaaagcagct ttagaagcag gtgaaatagc agcttccaga
    18241 gttggtacat taagaacatc acatattata ccgagaatcc ataatgaagt aacaaatgct
    18301 ttattcaaaa ctaaagaacc taaagtctgt gaagtttcag aagataaaga aaaaaacttt
    18361 gagattacat tggaaatagc tgataatgta tcgaaaaatt tatcagaaaa agaaaatgta
    18421 attttagatg tcaatgaaaa tagtggagat actgaaaatt tatatagtga tatagataaa
    18481 acaaacaaaa aaattgaaac agttgatgat agtttaacag aagctaatga ggaaaaaata
    18541 gaaaacacta atgataaaga agtattggaa caagttaaaa tagaaaattc cgattttgat
    18601 agagatgacc tatcaaagaa cagtgtaaaa aaacttaaag ctatggctaa gaaattagat
    18661 tcttcaataa cttataaaga cttaaatgtt cttaaaaaag aagaattaat aaacttagtt
    18721 aaaaagctta gtagaaggga taaatagtta tgacactttt agataaagat ttagtatcta
    18781 tacaagaagt aagaactctt gtaagaagat ctaaagtggc tcaagagaaa ctttctaaaa
    18841 tgagtcaaga gcaaatagat aaaatagtaa aagctgttgt agatgcagca tttaataatg
    18901 ctactaaatt agctaaaatg gcaaatgaag aaactggatt tggaaaatgg gaagataaag
    18961 taattaaaaa tacttttgca tctatgggag tttatgaaac tttaaagaac acaaaaacta
    19021 ttggaataat tggtgaagat aaagaagcca atacgataga tatagctaca ccagttggag
    19081 ttgttgcagg tcttatacct tcaacaaacc caacatcaac agtaatatac aaaactataa
    19141 tatctctaaa agcaggaaat actatagttt tctctccaca tccaaatgca aaaaactgca
    19201 tacttgaaac agttaatata ataagtgatg ctgctataaa ggctggcgct ccagaggatt
    19261 caatattatg tataactaat ccaagtatag ttggaaccaa tgaattgatg aaacataaag
    19321 atacaaactt aatactagca actggaggag aagcaatggt tagagctgct tactcttcag
    19381 gaacaccagc tattggagtt ggaccaggaa atggaccagc atttatagat aaatcagcaa
    19441 atgttaaatt agcaatcaaa agaataatag attctaaaac ttttgataat ggtgttatat
    19501 gtgcatcaga acaatctata gtaatagaaa aatctatgga agaaactgtt ttaaaagagc
    19561 ttaaaaatca aggtgcatat gttttaaatg aagaggaaag tgaaagatta gcaaaattca
    19621 tattaagagc taatggtact atgaatccac aaatagttgg taaaagtgtt gaggtaatag
    19681 caaatttagc agacttaaca aatgtaccta aaaatacaaa agttctagtt gcaagagaaa
    19741 ataaagttgg tcacaatgta gcctattcaa gagaaaaact tacaccaata ctagcgttct
    19801 atgttgaaga aaatgtagaa gctattatga acagatgtag agaaatattg ttaaatgaag
    19861 gtgcaggtca tacattctca atgcatgtaa atgatgaaga attggtaaaa agatttgccc
    19921 ttaatatgcc agtatcaaga atacttgtga atactccttc agcacttgga ggaataggtg
    19981 catcaacaaa ccttgtacca gcactaacac ttggttgtgg tgcaataggt ggaagttcaa
    20041 cttcaaacaa tataggacca cttgatttaa taaatgttaa aagagtagct tatggaatca
    20101 aagaaataga agaacttgga agagataaat ctcaagttga aaaatcaatg tttgactcaa
    20161 tggataaaga agaattaata aatcttcttg ttcaaaaact agtcaaagaa ctagcataac
    20221 taaaataaac aatgaaactt atagctatcg ctataaattt ctaaaaatat aaactataaa
    20281 aaactataaa attaaatttt tggaggataa gattatggca agtgcaaacg cattaggaat
    20341 gatagaaact aaaggattag taggagcaat agaagcagca gatgcaatgg ttaaagctgc
    20401 aaatgtacaa ttagtaggaa aagagcaagt aggtggagga ttagttactg taatggtaag
    20461 aggagatgtt ggagctgtta aagctgctac agatgcagga gctgcagctg ccgagagagt
    20521 tggagaacta atatcagttc atgttatacc aagaccacac tttgaagtag atgctatatt
    20581 accaaaggta tcagctgagt aatataagaa taattataaa atttaaattt tgattgaaaa
    20641 agaggctatc tcaaaaatga gtcttactaa taaactcaat gtattttgag gtagtctttt
    20701 ttatacatct aataggagat gtgataaaat gggtgtttta acagaaaacg aagtaaaaag
    20761 aaaaattgga aaaaacaaaa ataatataac tgaattttat atagaaaaag gtcaaataat
    20821 aactccttct gcaaaatctt atttagctga gagaagtatt gagcttaaat atgttgataa
    20881 actttcagat gtaaacaatg aacctaaaga aattgtaaaa gaagtagtta aagagaaaaa
    20941 atatagatat actactgtat ttggcgctaa attggatgaa aaaccagaac atatgactca
    21001 attaaatgga aatcttttag tttttaaaga ccataaaaga ataatattga gaggaaaaat
    21061 agattctctt gaatcagaaa tactaaaggt acagatactt tgtcagaaac tagatatgcc
    21121 aaagcttgtt aaggaattgc aagaagtatt agactttgta agaaatataa tgagatgtga
    21181 agttttagat gaaaagctag gggactttaa acttttagga ttaactcctc aagaacttag
    21241 agaacaatct cactatccta aaaaatactt tggtatagga catgagttcc caagttatga
    21301 aatgggagaa gtagtagtag ctataaatag tataagaagt aatacacgag aggttgaact
    21361 tgcagcctat gaagcattta agggtgagta tggaaatgtt cacagagaag atttaataaa
    21421 ggcacttaat agattatcgt cagtattttg gataatgata tataaggtta gaactaataa
    21481 gtataagtag ttggaggaca taatggagaa tctattaaat gaaatagtgg aagaagtaat
    21541 aactagagtt aaaaaagaag catttataga agttgaagca tcaggaagac acgttcactt
    21601 aagtagagaa gatgtcgata aattatttgg agaaggatat actcttacaa aactaaaaga
    21661 cctatctcaa ccaggtcaat atgcatgtaa ggaaagagtt acaataactg gtcctaaagg
    21721 aagtataaaa aatgtagttg tacttggacc atgtagaaat gaaactcagg tagaaatatc
    21781 tttaacagat ggttcaactc ttggactaaa agctcctata aaacaaagtg gagacttaga
    21841 aggtacttta agtattaaaa tatcaacaca atatggtgaa gttgaacttg ataaggggct
    21901 aatggttgct aaaagacata tacacatgac tcctaaagat gctgaaaaat tcaatgtatg
    21961 tgataaagag atagttgaag ctaaggtgat gggtcaaaga cctttaacat ttgatgatgt
    22021 agtaataaga gtgagtgaca gttttaaaac ttatatgcat atagactatg atgaagctaa
    22081 tgcatgtggt tattcaaaag gaactgttgc taaaataata aagaaagcat aaatttaacc
    22141 tactatataa attacctcta gggaggattt aatatgaatt atgataacct tgtagattta
    22201 ataacggaag aaatctataa aaaaataaat agtaatgaaa taaaaatatc taataaacct
    22261 aaagctgtga ttgtatttga acaagataac aataaattta acttattaaa agatgaattt
    22321 gaaatagtag aatttgataa aagtataagg gaatgtgaaa tagtgatagt ttcaagactt
    22381 tgtatgaggg ggctatcgaa tatagcactt ggaaattcaa caagtgatga agaaagattt
    22441 atactaaaaa tgattatgaa gggcaaaaaa gtatatgtcc tagatgaagg aattgaatac
    22501 aaaaaatata aggatactgc accaaaagca ttatataaga aatttatgtc atttgaagat
    22561 gaaatatgta aatttggtgt agaaattata aaagacttaa atgttataac taaaaataag
    22621 ttaaatataa agaaagaact agaaagtact agaggttcta aaaaagaaga tacaaaagtt
    22681 ttagataaag atgttttaaa tttaaccagt gtagaaagtg taaaaataga taatgaattt
    22741 agtttagatt taaggaataa aaaacttata tcggaagctg acttaagaaa accaactata
    22801 aatggagtta aaaacatatt agttaataaa aaaagtataa taacaccatt agctgtagat
    22861 ttcatgagaa tacaccatct caaactaaag aaactgtaag gaggtaattt tatggatata
    22921 ggaaaagttg taggtaatgt ttgggctaca agaaaagatg aaaaattatg tggccaaaaa
    22981 cttttagttg taaaaatttt aaaaactaaa gacacataca aggaaggctt catggttgca
    23041 gccgataatg caggagcagg aaatggagac ttagttctta tatccaaagg ttcctcagct
    23101 agagagtcga ttgaaaaatc tcatgttcca gtagatgcaa ctatagttgg agtagtcgat
    23161 tcgtttgagg tgtttgatga ataaaagtat aggagctata gagtttaaaa gcattgctaa
    23221 aggtatagaa gtatccaatg aaatgataaa gaaatcttca gttgatgtat tgtacttaaa
    23281 aagtatttgc ccaggtaaat ttctaataat agtaggtgga gaaacttctt atataaatga
    23341 atgtgtagat tatggaataa aacttggaga aggatatatt gtagataact tcgtaataaa
    23401 tgcaatttca caagaaatat tagatggatt taaaaataag tatcaaaagc tagatagtat
    23461 agtctctata ggcgttgttg aaagtagtaa aatatgcgct ggaataaaaa tgcttgataa
    23521 gactttaaaa tctggcgact tagtattagt aaaacttcaa ttatcttttg ctataggtgg
    23581 caaattagta tatatagtag caggagattt aagtagttta gaatatgcat taaaagaaag
    23641 tgaaaatgta gtaagagaaa aagaagtaat atataagact gtaataccat cagtagacag
    23701 tcaaataata aaaagtctaa taaaataatg gggggaatta tgagtataaa cgaaattata
    23761 atctatgtaa tggtagtctt catggtactt ggagctatag ataaatgtat aggtaacaaa
    23821 tttggtcttg gagaacaatt tgaggaaggt ataatggcaa tggggtcact tgcagttgca
    23881 atggttggtg ttatatgttt agcaccagtt cttgctgatg tattaagacc aattgtagta
    23941 ccagtattta atatacttgg agctgaccca gctatgtttg ctggaagttt attagctaat
    24001 gatatgggtg gtgcgccact tgcacttgaa cttgcgaaag accctaatgc tgggttattt
    24061 ggtggattaa tagtaggggc tatgatggga cctacaatag tatttataat accagttgcg
    24121 cttggaataa ttgaaaaaga agaccaaaaa ttcttagcaa caggaatact tgcaggtata
    24181 ataacaatac ctattggagc attcgttggt ggattagttg caggattcga ggtaatgatg
    24241 gttcttagaa acttagtgcc aataataata tttgcagtag ttatagcact tggattatta
    24301 aaattcgaaa atgctatgat aaaaggattc acatactttg gaaaaggtgt agtaatagtt
    24361 ataactttag gacttgctgc tgccatagtt gaagctttaa caggaatagt tgtaatacct
    24421 ggaatgaaac caatcagtga aggtatagaa atagttggag atatagcaat agttttagca
    24481 ggagcattcc cattagtgtt tgttataaca aaggtattta ataaaccatt aatggcttta
    24541 ggaaaagttc ttggaatgaa tgatgtatca gcagcaggcc ttgttgcaag tttagctaac
    24601 tgtataccaa tgtttggtat gatgaaagat atggacaata gaggaaaaat aataaatgta
    24661 gcattctcag tatcagcagc attcgtattt ggagaccact taggatttac ggcaggattt
    24721 aattctaata tgataacacc aatgatagtt gctaaattag ttggtggtat aacagcagta
    24781 ttgttagcta tggttatcgc aaataagaca ctaaagaagg agaatgtata agatggatat
    24841 atcaaatata gataaaaatt taattgaaac attagtaaga caaataatag aagaaaaaat
    24901 aagtggaact aaagacacag tagattttgt tagaaataaa gacattagtg gaataacttc
    24961 tataaagtta ccaactgtaa aagttagtga atctgataga ttagatacag ggaatccaag
    25021 tgatgtagtt tacacaaaag atttatttac attagaggaa agcccaagat taggttgtgg
    25081 tatgatggaa atgaaagaaa caacttttga ttggactcta aactatgatg aaatagatta
    25141 tgtaatagat ggaactttag atataataat agatggaaga aaggtttctg cttcttcagg
    25201 tgaattaata ttcataccaa aaggaagtaa gatacaattt tctgtacctg attatgctag
    25261 atttatatat gtaacatatc cggcagattg ggcatctcaa aattaataaa aatcaagtta
    25321 agttaataat aatcaaataa ataatttaaa atacaaactc ttaaataagc acacatattt
    25381 aaaaaagcta tgaattaaaa ttcatagctt tttttataaa gaatgttata ttttagacgt
    25441 attttttcta tatttactgg gagacatatt
//
LOCUS       DACXMZ010000033        24929 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.033, whole genome shotgun sequence.
ACCESSION   DACXMZ010000033 DACXMZ010000000
VERSION     DACXMZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.033"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            48..1904
                     /locus_tag="JD016_05335"
     CDS             48..1904
                     /locus_tag="JD016_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAU4999055.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    1987..2227
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2293..2528
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2613..3830
                     /locus_tag="JD016_05340"
     CDS             2613..3830
                     /locus_tag="JD016_05340"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HAU4999056.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            3947..4429
                     /gene="cdeM"
                     /locus_tag="JD016_05345"
     CDS             3947..4429
                     /gene="cdeM"
                     /locus_tag="JD016_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HAU4999057.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            4694..5959
                     /gene="hisD"
                     /locus_tag="JD016_05350"
     CDS             4694..5959
                     /gene="hisD"
                     /locus_tag="JD016_05350"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAU4999058.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6154..6906)
                     /locus_tag="JD016_05355"
     CDS             complement(6154..6906)
                     /locus_tag="JD016_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAU4999059.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFNY"
     gene            complement(7024..7293)
                     /locus_tag="JD016_05360"
     CDS             complement(7024..7293)
                     /locus_tag="JD016_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999060.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7768..8862
                     /locus_tag="JD016_05365"
     CDS             7768..8862
                     /locus_tag="JD016_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAU4999061.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            8864..9814
                     /locus_tag="JD016_05370"
     CDS             8864..9814
                     /locus_tag="JD016_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAU4999062.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            9807..10991
                     /locus_tag="JD016_05375"
     CDS             9807..10991
                     /locus_tag="JD016_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999063.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISNYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGMKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            11004..12500
                     /locus_tag="JD016_05380"
     CDS             11004..12500
                     /locus_tag="JD016_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAU4999064.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12523..13473
                     /locus_tag="JD016_05385"
     CDS             12523..13473
                     /locus_tag="JD016_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4999065.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13494..14456
                     /locus_tag="JD016_05390"
     CDS             13494..14456
                     /locus_tag="JD016_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAU4999066.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14828..15460
                     /locus_tag="JD016_05395"
     CDS             14828..15460
                     /locus_tag="JD016_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAU4999067.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15886..15963
                     /gene="kdpF"
                     /locus_tag="JD016_05400"
     CDS             15886..15963
                     /gene="kdpF"
                     /locus_tag="JD016_05400"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020062503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAU4999068.1"
                     /translation="MWFLAVVIIMLIIYLVYALINPEKF"
     gene            15992..17686
                     /gene="kdpA"
                     /locus_tag="JD016_05405"
     CDS             15992..17686
                     /gene="kdpA"
                     /locus_tag="JD016_05405"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAU4999069.1"
                     /translation="MGGTIFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFD
                     KVDNFIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSM
                     EQGLSFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGL
                     VGKKKTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDI
                     ARGPVAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGH
                     MIKNKKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGI
                     AQSSLFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAI
                     IAVFLCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGIS
                     NPGFHGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAIS
                     SLLASKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17711..19768
                     /gene="kdpB"
                     /locus_tag="JD016_05410"
     CDS             17711..19768
                     /gene="kdpB"
                     /locus_tag="JD016_05410"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HAU4999070.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            19787..20413
                     /locus_tag="JD016_05415"
     CDS             19787..20413
                     /locus_tag="JD016_05415"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HAU4999071.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNASYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            20650..21558
                     /gene="cysK"
                     /locus_tag="JD016_05420"
     CDS             20650..21558
                     /gene="cysK"
                     /locus_tag="JD016_05420"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAU4999072.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21585..22175
                     /gene="cysE"
                     /locus_tag="JD016_05425"
     CDS             21585..22175
                     /gene="cysE"
                     /locus_tag="JD016_05425"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAU4999073.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22335..22523
                     /locus_tag="JD016_05430"
     CDS             22335..22523
                     /locus_tag="JD016_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAU4999074.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22577..23446)
                     /locus_tag="JD016_05435"
     CDS             complement(22577..23446)
                     /locus_tag="JD016_05435"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HAU4999075.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23643..24893)
                     /locus_tag="JD016_05440"
     CDS             complement(23643..24893)
                     /locus_tag="JD016_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999076.1"
                     /translation="MIMENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFY
                     KKEKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLS
                     EFYDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNS
                     LSLVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDS
                     SIDIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEP
                     EFSDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETA
                     HAIDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDE
                     DSKNTLKENCPLTYDFLEKLELNY"
BASE COUNT         9604 a         2665 c         4506 g         8154 t
ORIGIN      
        1 attaagtagt attattataa ataacattga aaggtagtgg ttagaatatg gatacccata
       61 ataaatatgt aaattttata aaaaatatac ctgtaccttt tttatattgt agaattgtaa
      121 agagacaaga agatatagaa tatcgagtag aatatataag taagggtatg ggcaaagtat
      181 tacaattaga agaaggtatt tgtgataaaa atatactaga tgtattgcct gtatttaagt
      241 ctaaaaaata tttcaaagag ttgttctcaa atgaagtgga ttgtataaaa aggtacattc
      301 cgacacttaa aaactggata aatataaaaa aacagataat aggggattca tacataattt
      361 tgtattttgg taaaattgta tttgattata gacagataat cgattccttt gataaaaagg
      421 aaaaagtagc ttatattaaa gacgaagaag gcatatatat agattgcagt gaaaatttaa
      481 taccaatatt aaataacaac attaaaacaa cgaaagatat ctttggtaaa aatgatatag
      541 aagtatgggg agagaataca ggaaaattat ttagagatga ttatagagaa ggagtatcta
      601 gtaaaaaaag atttttacaa aatctttttg aatatgaaga aacatttttt atggttgaaa
      661 aatatttttt gtatgatgaa gacgaacttt taggtactat agggatagta gacaatataa
      721 tatatagtgg ttacagtaat agaaattata actcaaaaga tttaatgaag atgatagaac
      781 actcaattcc agagaatatg ttttataaag atgtgtatgg aaattatatt ggatttaatt
      841 caggtttttt gaatttagct tgtatgaata aagaagaatt attagggaag aatagttata
      901 aaatatcaga agaagaagct ttaatagata agatatttga aagtgataag ggtgtagttg
      961 aaaataaaaa agtagttaca tttgagttaa atataagtat gaatgatgaa aacaagtgca
     1021 ttgagattac aaaaaggccg ttttttgata gctatggaag tgttattgga ataattggaa
     1081 cagcaaggga tataagtaga agaaaaagat tagaagagga aatggataaa actaggatgg
     1141 aattttttgc aaatttatcc catgaactta gaacgcctat aaatttaata tcatcttcat
     1201 tacaagtgat agaaaaaaaa gaagcagatt taatagaatc aaatgatact ttaaaaagaa
     1261 atctaggtat aataaaacaa aatggaaata gaatattgag gttagtaaat aatgttatag
     1321 actttacaaa aatgcaatca ggatatttag attttaaacc agaagaatct gatataattg
     1381 catttataga ggagatatgt atgtctgtag ctgattttgc aagccaaaat aatatacaac
     1441 ttacatttga tacagaaata gaagaatttt caatgttatt tgattcagaa aaattggaaa
     1501 gaataatatt aaatttactt tctaatggaa ttaagtacaa taagaaagat ggaaaaataa
     1561 atatattttt atatgtgaaa gataatgtat ttaatatgaa aatatctgat agtggtatag
     1621 gtataccaaa agaaaaaata gataaaatat ttaataggtt tgaacagatt gacaatgaac
     1681 tttcttacag agttaaagga agtggtattg gactttcatt agtgaaatct ttggttgagt
     1741 tgcatgaggg aagtatatct ttaaaaagtc aacttggtat tggaagtgaa tttatagttt
     1801 cattgcctgt tagaagtaaa aataatattg aaaaatataa tcataaaaga gaaatcagca
     1861 atgaactaag taaaaaatta gaaatagagt tttcggattt ataagtttaa atataaaaaa
     1921 taaaatttta aaaaagtatt gacaattgat ttagaagggt ttaaaataaa ttacaacata
     1981 ataaataaaa aggcgatgaa taggaagagt aaatctaatt cgattaccag agagaagagt
     2041 ccgttggctg gaagactttt taaattagag ggattgaaaa ctacctagga gctactttct
     2101 gaagtcatct gcattagtag attttagtaa gaatagtcgt tagctgcgtt aaagtgtaaa
     2161 gtgagttttc atgacatatg aaaactaaat taggtggaac cgcgggaata atactctcgt
     2221 ccttatgtta taaggatgag agttttttgt tatttaaaaa tataaacagt aatcataata
     2281 attatagaaa atataaaagc aatgaatagg aagagtaaat ttaattcgac tatcagagag
     2341 gaaagttcac aggctgaaag actttctaag tttgagaaat tgaaaactac ctaggagctg
     2401 tggtctgaag ttactttaaa aagttttagt aagactaacc gttaactgcg ttaaagtgta
     2461 aagtggattt tatcaataaa tgataaaatc aaattgggtg gaaccgcgag tatagactcg
     2521 tccctttata tagggatgag tctattttta ttgcaataaa agattgaaaa taaaaaaaaa
     2581 tcggaaaatt ataaatttag gaggaaaata aaatggagaa taaagtgaaa attggagtac
     2641 taggatatgg agttgtagga agtggactta tagatataat agataataat aaagaaaaaa
     2701 gaaatataga aatagttgga attttagtta ataatttgga aaaacataag gacaaaaaat
     2761 attctaatat aataactaat aatatagatg atatatttaa taaagatata gatatacttg
     2821 ttgaagtcat gggtggttta gaaccatcat tatcttatat aaaaaaagca ttaaacaata
     2881 aaattcatgt tgttacagca aataaagatt tgttagctga atgtggagat gagcttgcaa
     2941 aattagcaag tgaaaataaa gtaagtatta aatttgaagc ttcagttgca ggaggtatac
     3001 cagtattaaa accaataata gaatcactag aaggaaataa tatagatagt ataaatgcaa
     3061 ttttaaatgg aactactaat tttatattat ctaaaatgta tgatgagaat ctatcttatg
     3121 atatggcatt aagacaagca caagaattag gttttgctga agcaaatcca gagtcagatg
     3181 tattagggta tgatgctgct agaaaactat caatattatc aactctagct tatgacaata
     3241 gagtatattg gaaagatgtg tatttagaag gaattacaga tatagatgaa aaagatattg
     3301 aatatgcaaa aaaattaaat tgtaaaataa aattgattgg tcaaagtaag tatgaaaatg
     3361 ataaagtaag tgcttttgtt agacctgttt tagtagaaaa agataatatc cttgctagaa
     3421 tagacaatga gttcaatgct gtcatagtga atggagattc agtaggagaa gtttcttttg
     3481 taggtaaagg agctggaagt ttagcaacag gaagtgcggt ttattcagat gttatagata
     3541 taatagataa tagagtttca agtatagatt cttttactaa agataaaata caagttaata
     3601 aaatagttag agaaaagtgt ggagcacttc ttagatttaa aaaatgtaat aaagatgaaa
     3661 ttctaaatat agttgagaat tgtttagtaa aattcgatat tttaaatgat gatgatgaac
     3721 ttgctattat ggtttatgct gattctgaat atgaaataaa caacagttta tgtctaataa
     3781 aagataaagg atactgcgaa aaaatgaata aaatgttgaa aataagttaa aaaagaaaaa
     3841 atataagttt atatgtaaca tattttgacg attttaatac tatatagtat gagatgaaac
     3901 aaaacatctc ataaaaaaat taaaattaaa gggagagata tgttatatgg aaaataaaaa
     3961 atgttattca gaagattggt atgaaagagg agaatctaca gctaaatggt tccaaaatga
     4021 tagagaagaa tatgaaagag aagcatatga tgaagataga gaaagaagag gttcaaactg
     4081 tggatgttca gattcaggag aaaatagacc tagaaactgt gaaagattta gaagagaagc
     4141 tgagataaga gaaagagaag caagagaagc attttgtgaa tcttcagaga aaaagaaaga
     4201 ggcattagca tatgaatgtg aagctagaaa attatgggaa gaagcagaaa aatactggga
     4261 tgaatattca aaatacaact ataaaggaat cgaatattta gcagaagctg ctagattatt
     4321 tgatgaaggt atggaatgtg aagctagaag aaatggaaat aatggaggaa acaataataa
     4381 ttgttgccat aaatgccata aatgtaattg taactgctgt agaaaataaa aaaattaatt
     4441 gacaaatcaa tagttaaaat atatcatatt atgtataata aaaagaaaat tcagaaaaaa
     4501 ataatgatta gaagaaatat aagtatattt attagacaaa tagtgctcag ttagtgaggt
     4561 gagtacaata tgattaaact cccatcctct agcttttgat agagggtggg agtttttatt
     4621 tattgatata tcttaaatga ggataatatt gtaaaataaa tgagtatgag attgtaaaat
     4681 taggaggtaa aaaatgaaaa gtattaaaac atcaagtatg aaagaaaagg tagatgaaaa
     4741 gatattaaga gagcaagtaa gttctataat agaagatata agaaataata aggatgttgc
     4801 acttaaaaaa tataatgaga agtttgatag aaatacaaga gatgaattta gaattactaa
     4861 ggaagaaata aaggaagctt ataagcatgt agatgacgaa tttataaata acttaaagat
     4921 tgctgccaaa aatataagag aatttgctaa agctcaaaaa ctaagttttg aaaacccttt
     4981 tgaaaaagaa atttatccag gtgtaatctt aggtcaaact aatattccaa tagagtcatg
     5041 tcttgcatat gttccaggag gtcagtatcc attgttttca acagctctta tgcttataat
     5101 acctgcaaaa gtagcaggag taaaaagaat agtggcatgt tcaccaacta tgaaaaatac
     5161 tgaaaagata aatcctaaga cattggtagc tatggatata gcaggtgctg atgaaatata
     5221 tgcaactgga ggagttcaag ctatagcagc atttacatat ggaacagaaa agattaatcc
     5281 agttgacata attgtaggac caggaaataa gtttgtaact gaagctaaaa gacaatgtta
     5341 tggacaagta gggattgatt ttgtcgctgg gccaagtgaa gttcttataa tagcggatga
     5401 aacttcaaat ccagtttata ttgcagctga tttattagca caatgtgagc atgatttaaa
     5461 tgcaagagga atattattga caaatagctt ggaagtagca caaaaagttg aaaaaaatat
     5521 agaaagtatg ctaaaagatt tgccaacaaa ggacatagca tatagttcat gggaaaataa
     5581 tggagagata atattggttg atgatatgga agaagccatt aaaataagta atttttatgc
     5641 accagaacat ttagaagttg cagtaaatga gtataatgat atttgtgata gattaactaa
     5701 ctatggttct ttatttattg gaaatctttc tgctgaggtg tttggtgact atgtttcagg
     5761 taccaatcat acattaccaa cacttaaagc atcaagatat actggtggtg tttgggttgg
     5821 aacttttatc aaaacttgta caaagcagat atttaatgaa aaagctatac aatcattagc
     5881 acctgtagct gaaaagctgg caaaagaaga gggattatat gcacatgcaa aggctgctga
     5941 ggttaggttt aaaaagtagt aaaaaatttt attttggcag tagtattaga ctgagataag
     6001 gaaaacgctg agaacctgaa taaatactat attttgtggg tctcggcgtt tcttttatat
     6061 ctgtatataa tagaataata ctatctgtgt gtttaaatac taacttaata tttcttttct
     6121 aaataatgtt tatgaaatag ttaatatttt tagttaatag ttgaaaaaaa cttttgctaa
     6181 ctgtgcaaat tcatctgcat ttgtcaatat agcagggtgt cctccctgac taaaaatgtg
     6241 aatacttgct tttgaaataa aagttgccat ttgtttgtac tcttcttcaa gattatgacg
     6301 acacatttcg tcttccttac ttcctgtaaa taaaattggc atttctaatt tacacaattc
     6361 tttatgaaac aaagggcgtt tctcgtttgc acattgcaat agagcttttg tatcaagatt
     6421 aacaacattc tcccaatctt ttccatgaca ccattcatag aattttcgtg cttgaatatc
     6481 ttgctttgat ttttcccttc cagtgattag attatctatg aagttatcat taagagtacg
     6541 tccatcaaag ctatctgcta taattgcttc aactaaatcg ggacgttcta atccagtatt
     6601 aacagctgcc catgctcctc cacttgttcc aattaagcca actttaccac aattcaaatg
     6661 ttcaatcagt gctattgtct gtaaggcttc atcatgccat atgtcaggag aaaattgaga
     6721 aactctatcc gactgtccat tacctaaaaa atcaattaag atacatctaa aattttctac
     6781 atataatggc acaagtgctt taaacatatt agaagaacca gtattaccat gcaggaaaat
     6841 tatcggttta ccttgtccat attctttata aaaacaagaa ttaccttgat actgaaaata
     6901 tgacatatat tttcctcctt tttatttgtt aagttaatta taacaatata ggtatgaatg
     6961 aaaaacctca tgttctttct tagaataaaa atgtgccacg cataaaacgt gacacatttc
     7021 taattataaa ttttgctgtt taagtattct ttgtatgctt ttctctgtca aaaagtattt
     7081 ctctgataac tgcttgatac tcattccatt ttggaaattt atacagattt gagaattacg
     7141 agaagctaag acttgtttgg tgtttgtgtt ttcaccccaa tgctttttgt gctcttgctt
     7201 tttgggaata taaatcactt taccatctat atattcttgc acttgctcaa tcagttcttt
     7261 aggcagtaag tcaatcgaat tttgatagct cattttgcac ctcctaaata attattcttt
     7321 aggattagca aaggctactg ccactcacaa ttaaaaataa aattttttat tatgtacaat
     7381 agcctttgct atgcacaagt tccaattccc ataaactaaa tactcctttc tggtttgaaa
     7441 aattcatctg aacattgatt tcgcttttaa gatagagttc aatgacaaat ttacattttt
     7501 attatatata ttcagataga taattgcaac tgtctttgta tgaggatatt ttattatagt
     7561 caatactata atatgtattt atatcatcaa actaatatga acagttgaaa ggaatatgaa
     7621 caataaaact tagtcaggaa gaataattat gaagcagaca atagagaaat ttgattttaa
     7681 agttttattg aaaatttatt gacaaattta acttgtaaat attatactaa acttaataaa
     7741 taaagtttat aaagtaaggt gttgtctatg aaaaatatag ttggaaaacc gcaaacgata
     7801 aaaaaagtaa atgaagattt aataaaaaac attataaaaa atgaaggtcc tataacaaaa
     7861 ccagaaattt caagcattac tgaattgagt ttagctacag ttaataagat tgtagagcaa
     7921 ttagctttga aaaatgaagt taaagtaagt ggactaagtg aatctactgg aggaagaaga
     7981 gctcaattat ttgaaataaa tgcaaactta gaacacataa tagctttata ttattataga
     8041 aattgttgta taggggtagt tgccaattta ttaggtgaaa taatttatca agaagaattt
     8101 aatataagaa tggatacata tgaaaacgtt aaccaagata ctttttttgt tatagatact
     8161 ctaataaaaa aggaaaaagg aaacaatata agggctattg gaattggtgt cccaggagtt
     8221 gtaaaaaaag gtgtagttaa aaatatacca actataaaaa gttgggaagg tataaattta
     8281 gaaaatttaa taaagagtaa atacaatact aaagtgttta tagaaaatga tacaaatatt
     8341 actactattg ggatttatca aaaacagtat aaagacaaat ataaaaatat ggctcttatg
     8401 tatttagaag atggtattgg ttcaggtata gttataaatg gtgaattgta tttaggttct
     8461 acaaattttg ctggagagtt aagttatcta aatatagaat ttggtaagta taactctaac
     8521 aacttaaatc ttgaagatta tattatgagt ttaagaaaaa gatatttaga aagtaaagat
     8581 aataaattta agaaagaaat tttatctata atatcaaact ttataaggac tcttgtatgt
     8641 gtcctaaatc cacaagctat gattatacag tgtagtatat taaccaagag tgacatggag
     8701 tttatagaaa aagatgtaag caataatgtt ggagaagaaa attttcctaa attaataaaa
     8761 gtagagtcat tgagagaagg aagtataaat ggagtaatta gtatgtgttt aaaagaaact
     8821 gactatcaat attccttatc taataaaaaa ggaggatatt aatgtgaaat atttggtttc
     8881 aatagatgga gggggaacaa aaactaaatt ttgtgtaagt gacttagatg gaaacatact
     8941 taaagaacat accactggtt caactaatta taagtcagtt ggaattaaaa aaacatatga
     9001 aaatattaat aatggattta aaaaaatatt aaaagattta tatatcgatt atgacgatat
     9061 tgagtacact gtatttggta tatctggttg tgattctcct aatgattata agattataat
     9121 ggatgaaata ttaaaaattg ggattaacaa agaaaaaata tatctggcaa atgatgcagt
     9181 tttagctttt tatgcacaag ctgattcccc tggtttagta attgtggctg ggacaggttc
     9241 aattatactg ggaattaaag aagatggaga aatatataga gttggtggat gggggtataa
     9301 ttttagtgat ttgggttctg gatatgatat agggagaaaa ttattaaaga aagtattgct
     9361 ttattgtgat gaatgtcatg agtatagtga tttatttagt tgtgtcttgg atttctttgg
     9421 agcaaatagt tttgaacaac ttgcatatat gattactgat ataaatgata atgttgaaat
     9481 agcaaactta gcttcattag tgattgattg tgccgaacaa ggtgataaat tggctattga
     9541 aatattaaga gaatcatcaa ctgaactatc taaattagct caagtaatat tgagaaaaat
     9601 atctaatcca atgaaaatgg ataaaagcga aataaatata gttttgtctg gtggtacatt
     9661 aaataaaact gtatatgctc aaatgttaat gaataattta aaaacagaaa atacagataa
     9721 aaatttaaaa tttatatctc aagaaaatca gccagtatat ggggggataa gacttgctat
     9781 ggcattagct aataggaggg tcaagcatgg atagaaaaaa ggtaatagta caaattttta
     9841 ctggaggttt taaggctaaa gaaataaagt ttaatgatat aaaatgtaaa ctactagaat
     9901 tattggactg tattgatata gaaaaggtta tcatgggatg gagtgtagat gaagaattat
     9961 atgtagaaac taaaaaacta ttaaataaat ataatgttga actatatctt tggttgccat
    10021 gtttttctga aataggatta cttgaaaaaa gtaatttact aattgattat atggggaagg
    10081 aagtcaaggg ctatgcttta caagaggatg aaaattttga gttttattgc ccaaataata
    10141 aggaaaatat aaaaaatata aagaaaatat atagtactta ttttagtgat attaaatttg
    10201 atggaatttt tttagataaa ataaggtatg gttctttttc gaataaacta aatggtgttt
    10261 ttaattgttt ttgtaaagat tgtttaaata tatatgaaaa aaataatatt gataaagagt
    10321 cgttaatgag agaaatgaat aatgtgtcta agggagttat agataatgaa gatactccat
    10381 ttggtataac agaatacatt aatggaaaat acaaatttaa aaataaaata tgggaagatt
    10441 tttttgaaac aaaaaatcaa aatatataca aggcattatt agagataagt aattatttta
    10501 aaagtaaggg attgaaaata ggtatagaca ctttttctcc ttttatttct tactttacag
    10561 gtcaagattt agaaaaatta caagaagtag cagattttat aaaaccaatg atgtatagaa
    10621 ctacaaaagc tccagcaggt ttaccatttg aatttgaaag gtttattgat gaatccatac
    10681 ataagaataa agagattgga aaaagaaatt tcttagatat tttaggaatg aaggagtatg
    10741 aaggagatgt atatcctata gaatttgtac tagaggaaat cgattttatg acaagaaatc
    10801 ttaataaaga tatatatgta ggagttgaaa taaataaaaa ggaaattata gctcctgtta
    10861 cacctaagta cataagtgaa aatatttcaa atataaataa gacaacagta aaaggatatg
    10921 ttttatcttg ggatttgttg tcagcaccaa aagaaaatat tgatgaaatt ataaaatggt
    10981 ttcggaatta gtgaggtgat aaagtgagta agatattgga aatgcaacaa atatataaaa
    11041 gttttggaaa ggtagatgtt ttaaaaggga taaattttga gttagaaaag ggagaagtaa
    11101 gagctctttt gggagctaat ggagcaggaa aatctacact tattaaaatt ttaggaggag
    11161 ttcattccca aactaaaggt aaagtaattt tgactggtaa tgaaataatg tttaaaaatc
    11221 cacatcattc taaagaaaat ggtattagta taatttatca agaattaagc ttgataccaa
    11281 cgcttacagt acttgaaaat atttttcttg gtagagaaat tttaaaagga agtgtgtttt
    11341 taaacaagaa agacatgctt gaggaatata tgaaaatttg tgaggatttt gattttaata
    11401 tagacccaca tatagtagtt tctaatttaa gtgtagcaaa ccaacaaatg gttgagataa
    11461 tgaaagctgt ttcatgtaat tctgaaatta tcataatgga tgaacctaca acatcactta
    11521 ctaataatga aaaagatggg ctatttaaaa taataagtag actaaagtct aaaggtaaaa
    11581 caatagtcta tatttctcac atattagagg aagtttttaa agtatgtgat agtgcaagta
    11641 taatgaaaaa tggaattatg attggaactt atgatgtaga ggatttaact aaattaaaga
    11701 taactcaact tatgacagga aatgataatc acaaacttat tgagaagaag gattatacat
    11761 ttgcaaaata tgaagagcca ccagtactag aagtaaagaa tataagctgg tctaacaaaa
    11821 taaaaaatgt ttcatttaaa gtttatagag gcgaagttgt aggtcttgca ggtcttgtag
    11881 gttcaagaag aactgaaatt ataaatttga tatttggtat agataataaa gatagtggag
    11941 acatttttat caatggtaaa aaagtagaca taacttctcc taaggttgct ataaaaaaca
    12001 aaataggatt gattcctgaa gatagaaaaa atctaggatt aattttagaa caagctatat
    12061 acacaaactc atcatcagta caaattgata aatttaagaa attcttgttt atagatagaa
    12121 ataaagaaat tgagttttct aaaaaaggtg ttaaggaatt aggaataaaa gttagtgatg
    12181 taaagcaaga agtaaaagag cttagtggtg gaaatcaaca aaaagtagta gtttctaagt
    12241 ggttagagaa agatttagat ttaataatat atgatgagcc gacaaaggga atagatattg
    12301 ctgcaaaaga agatattttc aatacaatca aagaattttc aagtaaagga ataggagtaa
    12361 tatttatatc ttctgattta gaagaagtaa taagagtaag tgatagaatt ttagtaacta
    12421 gagaaggttc tattgtagat gatttaaaaa atgagaatat acaggttcaa gatataatga
    12481 acagtatatt taatgtataa aaaatgaaaa tggaggactg agatgaagaa agcttttgag
    12541 aagttaaatt taagagatta tggagtatta ttaggttttt tagctttatg tatagctata
    12601 agtattgcaa ctccatcatt tttaggtaaa caaaatatac ttaatctttt acgacaatct
    12661 tctataattg gaataatttc agcaggtatg acttttgtta taatatctgg aaactttgat
    12721 atatcagttg gagcagtagc agctcttgca ggtgctataa caatgaaatt tgcgactggt
    12781 ggaactaatt tgtttgcatc catgctctta ggtgtagcga tttgtgcagt aataggatta
    12841 atcaatggta tagtagttgc aaagataaat gtaccatcat taatagccac tatggctatg
    12901 gtcactattg taagaggatt gctgttgatg cttacaggag gatatccaat tactgaaaat
    12961 ataccaatac ttgattacat tggaaatgga tatttgttta atataccaat accagtagta
    13021 attttcttta tagtggtgtt agtttcgttt attgtactta ataaaactaa gtttggtaga
    13081 tatgtttatt ctgtaggagg gaatcaagaa gcaagtaaac ttaatggtat aaatgtagat
    13141 tctcacaaag tgaaagtatt tattattaat gctgtacttg caggaatagc tggaatcgta
    13201 ctaacaggaa gacttgggac tgcaactgcg attgcaggtg aaggatatga tatggatgca
    13261 attgcatctg tagtaatagg aggtacatca gtggcaggag gttctggttc tgtacttaaa
    13321 actgttatag gagtattact tatgagtgtt ataaacaaca gttttaatct tcttggaata
    13381 gatgtatatt tccagtacat atttaaagga ttaataattt tagcagcagt tggatttgat
    13441 tcttatagca agaaaaaact tgcttcaaga taaaaaataa gggggatttt tttatgaaaa
    13501 aattaatgaa acatttagca ttgttgctat cacttgttat gatatttggt ttagttggtt
    13561 gttcaaatgg tggtgacaaa gaaaaaaatg gagaaaagaa aatagcagta ttactaccgg
    13621 gttcaacagg ttactttgta gcaactaaac aaggtataga tgcaaaatct aaagaattag
    13681 gtgtaagcgt ggaatatgca gatgctcaat gggatgctag taaacaatta tcacaagctg
    13741 aagattttat ggctaaaggg gtagacatga ttgcattatg tggagttgat tctgctgtaa
    13801 gtgagagaat agttaaagct gctaatgatt cagacgttcc tatagtagca tttacaaatt
    13861 ctataggaag taacccaact ggagaattta aaggtttagt tacatatata ggacaaaatg
    13921 aggaagaaac aggagcatta actggaaaaa ttgctaaaaa cttattagga gaaactggtg
    13981 ggaaagctgt attaatagaa ggagtaccag gaactactcc acaagtatta agaaaaaaag
    14041 gtcttgaaaa agaattaaaa gatagtaata tagaaatagt ttataatcaa acttcaaatt
    14101 gggagaaaga gcaagcttta aaagtaacag aagatttaat tcaaaagaaa actgatttta
    14161 atgttataat atgtcaagat gataattctg ctacaggtgc aggtcaagct ttaaaagatg
    14221 ctggtcttaa ggataaagtt aaagtcatag gtttgggtgg aagtaaagat ggattaaaag
    14281 ctataactga tgggttaata gatggtacaa cttatatgtc tgctgtagaa gaaggtggat
    14341 tagtcataga aaaggcatct aaattcctta aaggtgaaaa aatagaacct gttacacaaa
    14401 ttaaacaagt tgaagttaac aaagataata tttcagagtt caagggtgaa tggtaaattg
    14461 tcatagataa taaataagat ttttagagtt actttagaaa ctatataaag taactctttt
    14521 tttaatttaa tttagtgata aattattgaa tagtatttct aaacaaataa aaaattaata
    14581 taaacaaaga cttaattata ctaaattata agtaaaacta atataaactt atggaaaaaa
    14641 ggaaaacgtc taaagtattg gaattactat agtgtaggtg tggtattaaa aaaacataaa
    14701 aaaacataaa aaaattgttg aaaaaaatat agaaattttg tataattaaa gatttgaaaa
    14761 gtgcggtata caaagccaat tcttttgtat ataatatata taaattcaga aaaaggaagg
    14821 gatatgtatg gccaggcggt tatttttaga aaggattgga cttatatttt taggttcagc
    14881 aatattagca tttggagttt ataattttta ttatttaaac aacataacag aaggtggagt
    14941 gttaggtatt ttactactgt taaaaaattt attcaatata caaccagcaa tagcaaatgt
    15001 tgtaatagat ggtttactac ttttagtagg atataaattt ttcggtaaaa aattccttat
    15061 atattcaata gtagcttcta taactttttc agtgttatat gacttatttg aagctatagg
    15121 acctctagta ccacaatcac aaaatatgct tttatcaact attttggcag gtgtaactgt
    15181 tggaactggt gtaggtatag tagtaaaagc aggttgtgca tcaggaggag acgatgcttt
    15241 agcattagta atttctaaga ctacatcttt gaacataggg caagtttatt tagcaacaga
    15301 tgttattgta ttgttgttat cattatttta tctatcagcg tttgatatct tttattcttt
    15361 gattgctgtt acaattagtg gcaaggtaat tgattttatt tattatcatg gtaaaagttt
    15421 ggatatggat ataagtaatg atatagttcc agaatgttaa tataaattaa ataactttag
    15481 attttattag gggtggcaga tttgccacct tttttgtgtc taaatcttta tagaaagtat
    15541 aatcccatct aggtttttgt aaatctcaaa tatagaatta tgttggaatg actgggaata
    15601 cgattgctag atgtgcattt taaaaaccaa attttatatt tatgaaatac taatcattaa
    15661 tggatgtttc ttaatcctat ctttatacct aaaatgataa gataaatgaa gtagcaatac
    15721 aaaactaaaa tttgtaaaag agattaaatt aaatggagga gctaaaatgt tggatatagt
    15781 tatgatagca ttgttattca taggatttgt ttccatgaag ttacttgctg attggtgtgg
    15841 gaaacaaact gaaaaaaaat aatgtaatgt atggagggat tacctatgtg gtttctggca
    15901 gttgtaatta taatgttaat aatatatttg gtatatgcgc ttattaatcc tgaaaaattt
    15961 taaacagtat aaggctaata agtaagttga attgggggga acaattttta tggtgcagat
    16021 agtaatagtg ttagcgattt ttatgatttt agtaatacct atgggcaagt atttatatca
    16081 tatagctact aatcaaaaaa catttggaga tagactattt gataaagtgg acaattttat
    16141 atataaggta tgtagtatag ataagaaaaa agaaatgaac tggaaacagt atgcattagc
    16201 tttattattt acaaatgcag ttatggtatt tataggatat atcatactta gaactcaaag
    16261 tatgcatatt tttaatccaa gtggaattaa aagtatggaa caaggactat catttaatac
    16321 aataataagt tttatgacaa atacaaatct tcaacattat tcaggtgaat caggactttc
    16381 atactttagt cagatgacag tgataatata tatgatgttt acatcagctg caacaggata
    16441 tgcggctgct atggcatttg taagaggtct agtcggtaaa aagaaaacat tgggaaattt
    16501 ttatgtagat ttaattagga taacaacaag agtactttta ccaggagctt taattattgg
    16561 gcttatatta gttacacaag gagtaccaca aacttttgct gggacagaaa cagtgactac
    16621 tatagaagga aagcttcaag atatagctag aggtcctgtg gcagctcttg aatcaataaa
    16681 acatttagga acaaatggtg gtggattctt tggttcaaat tcatcacacc catttgaaaa
    16741 tccaacaata atatcaaata tagtagaaat attatctatg atgatattac caggagcttg
    16801 tgttgttgca tttggtcaca tgataaagaa taaaaaacaa ggttgggttg tatttggcgc
    16861 aatgtcaatc atatttttaa taggtctagt agtgtgcttt aaagcagaaa gtgcaggaaa
    16921 tcctatttta tcacaattag ggcttaatca aagtatggga agtatggaag gtaaggaagt
    16981 tagatttggt atagcacaat catcattatt cacaacagtg actacttcct ttacgacagg
    17041 tacagttaat aatatgcatg atacactgac tcctttagga ggattagtac cacttttaaa
    17101 tatgatgcta aatgtagtat ttggtggtaa aggcgtaggt ctgatgaata tgttgatgta
    17161 cgctataata gcagtattct tatgtggatt aatggtagga agaacaccag agtttttaac
    17221 taagaaaatc gaaggaaaag aaatgaagct tattgcttta cttattatat tacatccact
    17281 tttaatacta atgttctctg gtctttcagt agcaatacca gcagggcttg aaggaatttc
    17341 aaatcctgga ttccatggat tatcacaggt attgtatgag tttgcatctt cagcagccaa
    17401 taatggttca ggatttgaag gtctaggaga taacactatg ttttggaata taacaacagg
    17461 tatagttatg ttctttggaa gatatgtgtc tattatagta ctactagcaa tatcaagttt
    17521 gttagcatct aaaaaagctg taaatgaaag tataggaaca cttagaacgg ataattttac
    17581 atttacaata gttttagtac ttgttgtttt aattgttggt gctttaacat ttttcccagc
    17641 cttagcgctt ggacctattt cggagcatct agtgttgtgg cactaaaaaa ttgggaggga
    17701 gtaaagtttt atggaaacta aaaaaacaaa gtttattaca aaagatattt tcaaaacttc
    17761 cattatagaa gcttttaaaa aattaaatcc taaatatatg atgaaaaatc cagttatgtt
    17821 tgttgtagaa attggattta tagtaacatt gatacttaca atagtaccta gtttgtttgg
    17881 tgatgaaggc aataacttaa gagcttataa tgggattgta acaataatct tatttataac
    17941 agtccttttt gcaaattttg ctgaagctgt agctgaagga agaggtaaag ctcaagcaga
    18001 tacacttaag aaaactagaa aagatacagt agcaagggtt atagatacgt taggaaatga
    18061 aaaaatgata agtgcaagtg agctaaaact tggtgatatt gttttagtaa atgctggaga
    18121 ggttatccca aatgatggag aagttataga gggtatagct tctgtggatg agtcagcaat
    18181 aactggagaa tctgcacctg tcatgagaga atcaggtgga gatttcgcat ctgttacagg
    18241 tggtactaca gttgtaagtg actggttaaa gataagaata acaacaaaac caggagaatc
    18301 gttcttagat aaaatgatag ctcttgtaga aggtgcttca agacaaaaaa cgccaaatga
    18361 gattgcttta aatactttgt tagtagggtt aacacttata tttatggttg ttttaatatc
    18421 tctttatcca atggcaaact atgcaggtgt aaagattcca atttctacta tgatagctct
    18481 tttggtatgt ttaataccaa ctactatagg tggattgttg tcagctattg gtattgcagg
    18541 tatggataga gtaactagat ttaatgtaat tgcaatgtca ggaaaagcag tagaagcttg
    18601 tggagacgtt gatactatga ttttggataa gacaggtacg attacttatg ggaatagact
    18661 tgcatcagaa tttatagcag ttggaaatgc agattcaaaa gatttaataa attattctgt
    18721 tatgtgttca ttaaaagatg atacaccaga aggaaaatct atagttgatt taggtatgaa
    18781 attaggttca acaggtaaga caaaagaggc agaggaagct gagtttgtag aattttctgc
    18841 acaaactaga atgagtggta ttaacttagc tgatggtaga gctataagaa aaggtgctta
    18901 tgatagcata ataaaaagag taaaagaatc tggtggtaca gtgccagaag atttagaaga
    18961 aaatgtaaat cgagtagcta aactaggagg aacaccttta gtagtgtgtg tagataatga
    19021 gatatatgga attatctact taaaagatac agtaaaatca gggcttgtag aaagatttgc
    19081 tagacttcga gagataggaa taaagacaat aatgtgtaca ggggataatc ccttgacagc
    19141 agctacaatt gcaagagaag ctggagtaga tggatttatt gcagaatgta aaccagaaga
    19201 taaaatagat gcaataaaaa aagaacaatt agagggtaaa atagttgcaa tgacaggtga
    19261 tggaacaaat gatgctcctg cacttgctca ggcagatgta ggtcttgcaa tgaatagtgg
    19321 tacaacatct gcaaaagaag cagcaaatat ggtggattta gattcagacc caactaagat
    19381 attggaagtt gttgaaatag gaaaacagct tttaataact cgtggggctt tgacaacatt
    19441 tagtatagca aatgatgttg caaaatattt tgcgattata ccagctatat ttactcttgc
    19501 aatacctcaa atggagatat taaatgtaat gaaactttct acgtcattta gtgcgatatt
    19561 atcagcttta atatttaatg ctataattat cccatgttta atacctcttg caatgaaggg
    19621 agtaaaatat agacctatga aatcacaagc tatgttaatg agaaatatgc ttatatttgg
    19681 tcttggtgga gttatagtac catttttagg aataaaatta attgatttat tgattacacc
    19741 tttagtaatg gttcttaatt taggttaata aatggaggat ataaaaatga aaacattaaa
    19801 gccagcatta cttgtaagta ttgtattgtt agttgtttgt ggacttgtat atccacttgt
    19861 tttgacaggt gtaagccaag ttgcatttaa agataaagcc aatggaagta tgatagaagt
    19921 aaatggtgta aaagtaggtt cagaacttat tggtcaaagc tttacagatg cgagattctt
    19981 taagggaaga gtatcatcag taaactataa tacttataca aaagaagatt tagtaccaga
    20041 taaagatgga aatgcatctt atggaggtgt atcatcaggg tcttttaatt atggagctac
    20101 aaatccagaa cttcatgata gagtacaaaa agatatagaa aagtttttaa aggataatcc
    20161 aactgtcaaa agagaggata taccaacaga tttacttaca gcatcaggtt caggacttga
    20221 ccctaatata agtccagcct ctgcaaaaat tcagatacca gctatagcaa aagcgtctgg
    20281 aatatcagaa agtaaattac aaaaaattgt agatgataat acttccaaaa aactttttgg
    20341 agttttaggt gaagatagag ttaatgtact aaaggttaat gtagaggtcg caaaaatatt
    20401 aggtcttata tagtattttg tagaatttta gtaacaaaat attactcaag atttaattta
    20461 aaggttaaat tttaaagagt gaaaatcaat aatgattttc acttttttat ttgaatatta
    20521 ctaatatttg gaataaagtg attgtaagta taaaatccaa aaaaaggttg cattatcaaa
    20581 atgataatac tataatatat ttatcgttat caatttgata atagatttgg acatatagga
    20641 ggaaatagta tgttatataa taacgcatta gagttgatag gaaacacacc tgtagtaagg
    20701 ctgaataatt taggctgtac taatgggtgt tctaacatat atgtaaaatt agaaaaagta
    20761 aatccagcag gaagtataaa agatagagct gtttatgaaa tgttagaggg tttagagcaa
    20821 agaggagaat taaaaaaggg agatgtacta gttgaagcaa ctagtggaaa tactggtatt
    20881 gcattatcta tgataggaaa attaaaagga tatgaagtag taatcgtaat gcccgaaaca
    20941 atgagcgttg agagaagaga tttaatgaag gcttatggag caaatttggt gcttacagat
    21001 ggtaaagctg gaatggcagg ttccatagaa aaagcaaatg aacttttaaa agaaaatcca
    21061 aactataaaa gcttaaaaca atttgaaaat aaagataatc caaatgcaca ttataaaaca
    21121 acagcagttg aaattatgaa tgatgtcaaa gatttagata tattcgtatg tggagttgga
    21181 acaggtggta ctttaatagg aactgcaaga tatttaaaag aacaaaatcc aaatataaga
    21241 gtaattgcac ttgaaccaaa aaaatctcca gctatatcag aaaacaagac tggacctcat
    21301 aaaatacaag gaataggtgc taactttgtt cctgaaaatt atgattctag tgtagttgat
    21361 gaagtaatat tagtagatga tgaagatgca tttgatacag ttaaattact tgcagcaaaa
    21421 gagggaattt tagtagggat atcttctgga gcaaatatat ttggtgctat agagatggca
    21481 aaaaagtatc cagataaaaa gatagttaca gtagctccag atggagttga taagtatatg
    21541 tctatgggaa tattttaata attttatttg agggagtgaa tactttgttt aagaaaataa
    21601 ataaagatat tgaatatata atgaagaatg atccagcagc taggtctaag atagaagttt
    21661 ttttacttta tccatcagtt catgcaatga taatgcatag gatggctcat gctctttata
    21721 agaagaagaa gctttttaca gcaagattaa tatcccagat atccagattt atgactggta
    21781 tagaaataca tcctggtgct aaaatggggg aaggtatatt gatagaccat ggaatgggtg
    21841 ttgtaattgg agagacagct gaagtaggta atagggtgac aatatatcaa ggagctactt
    21901 taggagctac aggaaaagat actggaaaga gacatccaac tgttggtgat gatgtattaa
    21961 ttggtgctgg aacaaaaata ctaggacctt taaatatagg ttcaaattca aaaattggtg
    22021 caaattctgt tgtcgttaag gatgtaccta atggtgcaac agttgttggt ataccagcaa
    22081 aaatagtaaa aataagaaac ctagaacctg taaaaaagaa taaaaaagaa gtgtcctatg
    22141 agtatgatga attagataat gtatattata tataggattt atataactat aaaataaact
    22201 ttataaaaat tattttaaaa ggtatttttt tcaaatttga ggacataata aaaaagaact
    22261 gaatgttaca gttttggttc atcaatcttt gataaacaag attttaaatt tgtagcgtct
    22321 aggaggattt tattatgaaa gcaaatgtta atcaagatac ttgtataggt tgtggattat
    22381 gtccatcaat atgtccagaa gtatttgata tgaaggatga tgggaaatca catgttatag
    22441 ttgatgaagt accaagtgat gctgaagaat cagcagcaga agctagagaa agctgtccag
    22501 ttgatgcaat agatgtgcat taataaaata aatgcctata aaaattgaat tttcaatttt
    22561 tataggcatt tgtcttttat tcagttagta tttcaacacc tgtttctgta actaaaacca
    22621 tacattccca ttgagcagat aatttaccat cttttgtata aatagtccaa ttattttctt
    22681 catcaacaaa aatttcgtga gttccttcat ttaccatagg ttctattgtg aatatcatcc
    22741 caggaaccaa tagcatctca gtcccttttt tagatacata acttacaaaa gggtcttcat
    22801 gaaagcttaa tccaactcca tgaccaccta tttctctaac aacagagtat ccatttttct
    22861 ttgcataaga gttgatagca tcacctatat ctcctaaaaa tccccatggt ttggcaactt
    22921 caagaccttt ttggacacat tcctttgtaa cttgaactaa tttttttgca ttttcattaa
    22981 cattacctat cataaacatt cttgaagcat ctgaatagta tccattaaaa atagtagaaa
    23041 catctacatt tataatatct ccatctttta agataatgtc cttacttgga ataccatggc
    23101 aaacttcatt atttactgat gtacatacac ttttaggaaa tccattatag tctagtggag
    23161 cagggatagc tccattttgt agtgtaaagt catgaaccaa cttatttata tcttctgtag
    23221 acattccaat ttcgatttta tctgaaatat aatccaaaat ttttgtgttt attacagcac
    23281 ttttttttat ttcctcaatt tgactttcat tttttataat atcatgagtt ggtacaatat
    23341 gacctaactt agcaaatgaa tctattttat tatccatatc catatgacat tttttatatt
    23401 ttttagtact tccacaccaa cagctatcat ttctatttaa cttcataact tttcatcctt
    23461 cttttattat ttatttaaat tatgggttac ataaactatt ttaaaacata accattataa
    23521 atattataac ataaatacta agaaatcctt tatagatagt gatttattca aagtataaat
    23581 tattatatgg ttattgttga tgaaaagagg atagttaaat gtattattgg atattagcgc
    23641 acttaatagt ttagttcaag tttttcaaga aaatcatatg ttagaggaca attttccttt
    23701 aaagtatttt ttgaatcttc atcaagataa taatgtacaa atgattctgc aaaaaattct
    23761 tctataaact tgcttatata tgcatgtgca acttgcttag ggtcataaag cttatatctt
    23821 tcctgagcaa atacttccat aaattcttca gaatttgatt ttttatttaa aacattttta
    23881 tctatagcat gtgcagtttc atgtaattct aaattttttg atgaatgtac atttgcatag
    23941 ctaggattac tataacctat tttagccact atagaattat ttctatatat accaggtaca
    24001 tctttccaag tcttgcctga tcttacccaa catggaggta attgatattt taaatcacta
    24061 aattctggtt catcagttag atttgaattt attaatttta tttttatatc cttttcttta
    24121 agtgtctgta aatatttaat atttattttt gcaagtctgt ttttcatatt ttcaacttca
    24181 tctttatcat aattcaagtc atcaggaagt ataagcattt gttcaattat atctatacta
    24241 ctgtctttta tattttcaga ttcttcatca aatttatatt gaagagtatt attttcatag
    24301 atagttatat ttgtattatt ttctatagaa taagagtgaa tatcctctaa atctaaaata
    24361 ttcatttgtt gtaaaatatg ttttgtttcg ccactccaca ctactaaaga aagtgaatta
    24421 aaattaatta gtggattttc ttttgctttt atatcattgc ttatatctac atcaaataca
    24481 tattcaattg agtcttctat gttaagacta tctgtattct ttttaatata taaatacgct
    24541 tgttttaagt ctatataaat ctcatctaaa gattcgtcat aaaattctga taaattatta
    24601 gtaaagagtt cttcacttaa aagttcattt ttatcagtta tactttcaat taaaatatta
    24661 atttctttta tcatatcgta aatacttatt tttctatttg ctttttcact agatacattg
    24721 catggcgcaa gagcaatgca ataaaaatct ttttcttttt tataaaaggc tttaagtgat
    24781 tttgataaaa gtgtaaagtc tacacctatc tgtaagttga ccttaagtga gtcattttgt
    24841 aataattcag tattatttct tttagctgta tttaaattgt tttccataat cattgccccc
    24901 tttttattag ggtatcataa tttttttat
//
LOCUS       DACXMZ010000034        24548 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.034, whole genome shotgun sequence.
ACCESSION   DACXMZ010000034 DACXMZ010000000
VERSION     DACXMZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.034"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     regulatory      2..87
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            494..811
                     /locus_tag="JD016_05445"
     CDS             494..811
                     /locus_tag="JD016_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HAU4999077.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            819..1223
                     /gene="flgC"
                     /locus_tag="JD016_05450"
     CDS             819..1223
                     /gene="flgC"
                     /locus_tag="JD016_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAU4999078.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            1233..1550
                     /gene="fliE"
                     /locus_tag="JD016_05455"
     CDS             1233..1550
                     /gene="fliE"
                     /locus_tag="JD016_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAU4999079.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1575..3125
                     /gene="fliF"
                     /locus_tag="JD016_05460"
     CDS             1575..3125
                     /gene="fliF"
                     /locus_tag="JD016_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
                     /protein_id="HAU4999080.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISIDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEQEDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            3138..4208
                     /gene="fliG"
                     /locus_tag="JD016_05465"
     CDS             3138..4208
                     /gene="fliG"
                     /locus_tag="JD016_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAU4999081.1"
                     /translation="MSTEITNVNAFDLSDISESDKNEKIEGEGLKKAAVLLMTLGPDV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAIRISSMGSISPEVIKVIESSLEDKLHSISGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDAISDAIYRNQSSRAAEALREEMEMVGVIKVTQVEAAQQKIVNS
                     VRKLEREGAITIVRNFGDDFVE"
     gene            4201..4932
                     /locus_tag="JD016_05470"
     CDS             4201..4932
                     /locus_tag="JD016_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAU4999082.1"
                     /translation="MNNKIIKSKKASYKGVLDLSIIDKVRVGESNLNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFKLFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            4936..6255
                     /gene="fliI"
                     /locus_tag="JD016_05475"
     CDS             4936..6255
                     /gene="fliI"
                     /locus_tag="JD016_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HAU4999083.1"
                     /translation="MYKTDIYIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            6292..6732
                     /gene="fliJ"
                     /locus_tag="JD016_05480"
     CDS             6292..6732
                     /gene="fliJ"
                     /locus_tag="JD016_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HAU4999084.1"
                     /translation="MDKNFKFRLQKVLDLKVKDEEEIKMEFAKIQQKKIDIETNLENL
                     ESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLISKQ
                     IERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            6761..7966
                     /locus_tag="JD016_05485"
     CDS             6761..7966
                     /locus_tag="JD016_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HAU4999085.1"
                     /translation="MKIQADSYNIIKDIGDRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRREVSKQNISSDKPENSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENII
                     DDDRVDLLA"
     gene            7984..8592
                     /locus_tag="JD016_05490"
     CDS             7984..8592
                     /locus_tag="JD016_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HAU4999086.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            8619..9602
                     /locus_tag="JD016_05495"
     CDS             8619..9602
                     /locus_tag="JD016_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HAU4999087.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQIYAFQNPAGLDAAGGNLFKPSANSGNPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9615..9818
                     /locus_tag="JD016_05500"
     CDS             9615..9818
                     /locus_tag="JD016_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlbD"
                     /protein_id="HAU4999088.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9818..10636
                     /locus_tag="JD016_05505"
     CDS             9818..10636
                     /locus_tag="JD016_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086725.1"
                     /note="Homolog of MotA, appears to be involved in motility
                     on surfaces and under different ionic conditions. With
                     MotS (a MotB homolog) forms the ion channels that couple
                     flagellar rotation to proton/sodium motive force across
                     the membrane and forms the stator elements of the rotary
                     flagellar machine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HAU4999089.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10626..11321
                     /locus_tag="JD016_05510"
     CDS             10626..11321
                     /locus_tag="JD016_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAU4999090.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11333..11776
                     /locus_tag="JD016_05515"
     CDS             11333..11776
                     /locus_tag="JD016_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HAU4999091.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11791..12165
                     /locus_tag="JD016_05520"
     CDS             11791..12165
                     /locus_tag="JD016_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HAU4999092.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            12166..12831
                     /gene="fliP"
                     /locus_tag="JD016_05525"
     CDS             12166..12831
                     /gene="fliP"
                     /locus_tag="JD016_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086729.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAU4999093.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12844..13113
                     /gene="fliQ"
                     /locus_tag="JD016_05530"
     CDS             12844..13113
                     /gene="fliQ"
                     /locus_tag="JD016_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAU4999094.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            13125..14939
                     /locus_tag="JD016_05535"
     CDS             13125..14939
                     /locus_tag="JD016_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HAU4999095.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGVNETITGIMLEYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            14948..17023
                     /gene="flhA"
                     /locus_tag="JD016_05540"
     CDS             14948..17023
                     /gene="flhA"
                     /locus_tag="JD016_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAU4999096.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16998..18014
                     /gene="flhF"
                     /locus_tag="JD016_05545"
     CDS             16998..18014
                     /gene="flhF"
                     /locus_tag="JD016_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HAU4999097.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQVINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            18011..18889
                     /locus_tag="JD016_05550"
     CDS             18011..18889
                     /locus_tag="JD016_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HAU4999098.1"
                     /translation="MIDQAQILRKIAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18903..19604
                     /locus_tag="JD016_05555"
     CDS             18903..19604
                     /locus_tag="JD016_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HAU4999099.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19620..20042
                     /locus_tag="JD016_05560"
     CDS             19620..20042
                     /locus_tag="JD016_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HAU4999100.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            20027..20212
                     /locus_tag="JD016_05565"
     CDS             20027..20212
                     /locus_tag="JD016_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HAU4999101.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            20253..21026
                     /locus_tag="JD016_05570"
     CDS             20253..21026
                     /locus_tag="JD016_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HAU4999102.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            21047..21808
                     /gene="flgG"
                     /locus_tag="JD016_05575"
     CDS             21047..21808
                     /gene="flgG"
                     /locus_tag="JD016_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HAU4999103.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21831..22712
                     /locus_tag="JD016_05580"
     CDS             21831..22712
                     /locus_tag="JD016_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAU4999104.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVRDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDIGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22715..23089
                     /locus_tag="JD016_05585"
     CDS             22715..23089
                     /locus_tag="JD016_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAU4999105.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            23121..24524
                     /locus_tag="JD016_05590"
     CDS             23121..24524
                     /locus_tag="JD016_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAU4999106.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISSGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDEAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFERYNQLIRLINRGMYKEAIQGFKLITEQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
BASE COUNT        10150 a         2617 c         4614 g         7167 t
ORIGIN      
        1 taaataggca aatctagaga aatctagtga cgcaaagcta tagggactaa ggtttatata
       61 cataaactat gtcagccagt tgccaaaaag agtcctaggt gtattgtata cctaagaaaa
      121 gtctataatg actgcctttt tggcagtcat tttgttatgg aaaaatcact ttatttcagt
      181 agaaaataca tataataaca tattttacca attataacat aaaataaaga aaaattatct
      241 caaaaagtta ctattttaca aaaaagagca acttttcgaa gaaatattta aatacatatt
      301 aaaatttttt tatattttca tgggacttca ttaaaaaaat attagttttc ttactaaatt
      361 gatacattat tgtattaatg taatggaaat gcgattaatt taaataaata aagaagtcat
      421 tttttgtaat atagcaactt cattttttat taataaacaa aatagataaa atatattttg
      481 aggaggggtg agtgtgaata catatgacat tataaaaatg ggattagatg caactaatct
      541 aagaagtcag acaatagcaa ataatatagc aaatataaat actgaaggat ataaaagaca
      601 ttatataact tttgaagaaa cactaaatga tgctatgcct aaaatagaag aaaaaacaga
      661 caattctaaa ggtttaagag tagatggtaa taatgtagac atagaagaag aaaaagtaaa
      721 tcaagcaatg gcaacattac aatataatgc tttgataagt tttgcaaatg gaaaaatagc
      781 aatgaccaaa tcaataataa gtgggaggta attagaagat gagtgtgttc agtggaatga
      841 gaatatctgc tagtggatta acagcagaaa gtttgaaaat ggatatcata tcatccaatg
      901 tagctaatat aaatgctaca agaggtccaa atggagaagc atataggaga aaagttgttt
      961 catttgaaga aaactatgat aataaattag gtatgctagg agtaaaagtt acatctgttg
     1021 aagatgataa atcacctctt aaagaagtat atgacccatc tcatccagat gcaaagccaa
     1081 atggatatgt agaatatcca aatgtaaata tattggtaga aatgacagat ttaataacag
     1141 cttcacgagc atatgattct aacgtagata cattgaatgc tcagaagtct atgatatcaa
     1201 aagctttgga aataggaaag tagggataaa ttatgccaat tacacaaatt aacaatttaa
     1261 tgcaaggtgc aaagttagat aatttgattg gagaagcaaa gccattaact cttaataata
     1321 aagaagaaaa aaagaatttt gaagatgtaa taatagatac tatagataaa gttaattttc
     1381 aacaagtaac agcagatgat atgaaagaaa aatttataaa aggcgaagat gtttctatgc
     1441 atgaagttat gttaataggt caagaagcac aactatctat gcaatattta atagaagtta
     1501 gaaataagat tcatgatgca tatcaagaaa tgaataaggt gcaaatttag ccttttgaac
     1561 aggagtaata atcaatgata accagtttta aaaataagat taaggagcaa gttgctaaat
     1621 ctaggacaaa gataagttct ttaaaaaagg gaactaaaat agcattaggt ataggggtta
     1681 ttgctatatg tttagcgcta gtatttacag caatatctgc gagcaaaaat aagtatgcag
     1741 tcttattttc agaactagat gctcaagatg ctgcagatat atcagcagag ctagagtcta
     1801 aaaagataga tactaaggta aaaggaagta cgatttatgt acctagaaca gaagttgata
     1861 agttaaggct ggagttagcg ccaagtctta caggtggttc taaaggattt gcactaatgg
     1921 atgatagttc atcaataggg cttactgatg aagaatttca gattaagaaa caaagaatga
     1981 tacaaggtga aatagaaaaa acaataaaga gttttccaca agtggaagac actagagtac
     2041 atataacatt tggagagtca tcagtatttg agaaggaggc tgttccaggg agtgcagcag
     2101 tttatttgat gctaaaacca gggacgactt tagaaaaaaa ccaaataaag tctgttatgg
     2161 cacttatatc aggaagcgtt acaaatatac cagagaaaaa tgttcaggtt attgataaca
     2221 aaatgaattt attatctgaa ggtattttgg aggatgaaaa tgttgatgaa aataatcaac
     2281 caataaatag tggcattagt attgataaaa ataaggcatc tgaacaagca ttgaataaac
     2341 aactagaaaa gtctatttta gaaatgctag aaccgatatt tggtaaggga aaggtgaaag
     2401 ctacagtaaa tgctgattta agttttgata cagctgaaaa aactgagata aaaattgacc
     2461 ctgataaagt tgcaataaag gagacaaggt cgaaaaattc atctaaatct gctgcaaata
     2521 atgttcaagg tgtagatgct aacatgaata accaaggaaa taacaatggt actaatacag
     2581 aagatagttt agatgagagt tttgagtatg aaacaggtaa gattgaaact cacactatag
     2641 ttgcgcctgg agaacttaat agattgacag cttctgttgc aatagatgga aaaatagcta
     2701 aaggtgttca agctgatgta gaggatattg ttaataatgc tataggtatg gatggagcga
     2761 ggggagatac tcttagcgtt gtttcaatgg tatttgaccc agaaggtaag aaagaggcta
     2821 aacaagagct ggaagagatt aaaaaagaag gatttaaaaa gaacataata aaagcagtta
     2881 ttggagtagt agcagtaata gcagttggag ctattggata cttaatatat aagaaaagaa
     2941 gtgaacaaga agatatggat tatgaagatg aagattatat ttctaaatct gattctgttg
     3001 ataggaaaga taatcctcta ttaggagatg acccagaact gacattagag gaagctgtta
     3061 aaatgtatgc agaagaaaag ccagaccaag taacagagat aataaaaact tggttaaatg
     3121 agtaaggggg atagaaagtg tccacagaaa ttacaaatgt taatgcgttt gacttaagtg
     3181 atataagtga aagtgataag aacgagaaaa tagaaggtga aggtttaaaa aaagctgctg
     3241 tattattaat gacattaggg ccagatgtct ctagcgatat aataaaaagt ttgcctgata
     3301 aacaagttca aaaaataggt ttggaaattg caaatatttc ttcaatagct tctaaagaaa
     3361 gaagagaaat cttaaaagag tttgtagaaa taaatagagc aaaagattat gttatagaag
     3421 gtgggcttga atatgcaaga gagttattaa gtaatgcttt gggaaatgct aaagcaaaca
     3481 aattacttga aggtatatca ataaatacaa gtacaaagcc ttttaaacat attatcaaac
     3541 tagatgtaaa gaagatatta gaaatactta ggaatgaaag tgcacaaact atagccgttt
     3601 tacttgcaca tatggaacca gaaaatgggg cagaagtttt atcatattta gactttgatt
     3661 tacaaaatga agtagctatt agaataagtt caatgggttc aatatctcca gaagtaataa
     3721 aggtaattga aagttcatta gaagataaat tacattcaat aagtggtgat gattttaagg
     3781 attatggagg agtgtctaca ttagttcaaa tattgattaa ttctgataga aaaacagaaa
     3841 agaatatctt gggaactatt gaagaacaaa acccggattt agcagctcaa attaaatctc
     3901 atatatttgt ctttgaagat attgtcaagt tagatgatat aagcatacaa aaagtgttaa
     3961 aagaagtaac tatgaaagat ttagtatatg ccttaaaagg tgtggatgat gcgatttcag
     4021 atgctatata tagaaatcaa tcttctaggg cagcagaggc acttagagaa gaaatggaaa
     4081 tggttggtgt tataaaggtt acacaggttg aggctgctca acaaaaaata gtaaatagtg
     4141 taagaaaact tgaaagagaa ggtgccataa ctattgtaag gaattttggt gatgactttg
     4201 ttgaataata agattattaa aagtaaaaaa gcatcatata aaggagtttt agatttatcc
     4261 ataatagata aagttagagt gggagaatca aatctaaata aagaaaatga ttgtagattt
     4321 agggatgagg taataaagga agctatagaa tctgcagaga taaaaaagaa tgagatatta
     4381 gaagaagcaa aaatgaacgc acaaaatata gaaaagcaag catatgaaaa agggtatagt
     4441 caaggtcaaa aaaatggata tgaggatgga tataaagagt catatgatga atatacccaa
     4501 aaagctaaag atgaagcaga ttttataaaa aatcaggcaa atttaatctt attacaagct
     4561 aatgagaaga tgactgagta cataaaagaa aaaaataaag agattgtaag acttgctatt
     4621 aatatggcca gttctgtttt aaaaaagcat ttcgaagatg aagaatctat gagtgatttg
     4681 ttaaagcaaa ttattcaaga ttatgaagga agtagttcat ttatagtaag atgtaattct
     4741 ttctataaag ataatataga gaaagaattt aaattattta aagaggaaac atcactaaaa
     4801 tcaagtgtat ttgttattgc agatgatagt atagataaag gaaatgctat tatccaaaag
     4861 gaaaatggaa gaataatagt tggtattgat tgtgctatgg aaaaactaaa agaagaattg
     4921 cttggtgaat aaagtatgta taaaactgat atatatatag attttgacaa aataaataag
     4981 aaaatagaaa atttagaact cactatatca gaaggtagag taaagaagat aataggactt
     5041 actgtagaag ttgaaggaat aaaagctttt gttggggagt tatgcgttat atacaaccaa
     5101 gttaataaac cagtgaattg tgaagtagta ggttttaaag ataaagaagt catattaatg
     5161 gcgctgggag agttaacatt gatagctcct ggttgtaaag ttatttctaa ggggatacct
     5221 ttaagtgtta tgtgtagtga taatttacta ggtaaagtac ttgatggtct tggaaatcct
     5281 atagacaact cagatgctgt tttaggagat agatacaacc tgaacaatga acctcctgac
     5341 ccaatgaaga gaaaaaagat acgaaatata atggaaacag gtgtaagagc aatagatgca
     5401 tttactactt gtggtgaagg acagagaatc ggcatatttg caggaagtgg agtaggtaag
     5461 agtacgactc taggtatgat tgctaggaat gcaaaagcag atgtaaatgt aattgctctt
     5521 attggagaaa gaggaagaga agtactagat tttattgata aagatttagg tgaagaggga
     5581 atgaaaaaat ctgttgttgt gtgtgcaaca tcagataaag cccctctagt taggttaaaa
     5641 ggtgctctta ctgctactgc aatagcagaa tattttagag accaaggcaa aaaagtaata
     5701 cttatgatgg attcagtaac tagatttgct atggcacaaa gagaagttgg tgtagcaata
     5761 ggagagccac cagctcaaaa aggatataca ccatctgtat ttgcaatgtt gccaaaactt
     5821 atggagagaa cagggacatc agataaaggt tctataactg cattttatac agtattagtt
     5881 gatggagatg actttaatga gccaatagca gatacaacaa gagggatact tgatggtcat
     5941 atagttttat ctagagactt ggcaaataag aatcactatc cttctataga tgtattaaac
     6001 agtttaagta ggcttatgaa tgaaattgct tcaaaagaag acataaaaat agcgtctttt
     6061 gcaagagaca tgcttgctga gtatagagaa gcagaagatt tgataaatat aggtgcatat
     6121 gcttcaggta caaataaaaa aatagatgaa gcaatctatt atcatgagca tattattaat
     6181 tttttaaaac aagggataaa tgaaaagagt tcttttaatg aaactataag tagtttgaga
     6241 agagtttttg aataaacaat taaaaaaata tttgaatagg ttgtgatgga attggataaa
     6301 aactttaagt ttagattaca gaaagtttta gacttaaaag tgaaagatga agaagagata
     6361 aaaatggaat ttgctaagat tcaacaaaaa aaaatagata tagagactaa tttagaaaac
     6421 ttagaaagta attatagcaa atattctata agtaaaaata acgatagtat tcaaaatcaa
     6481 aaaataacta ttaactattt actagcttta aacaattcta taatggattt atcagaagag
     6541 ttggataagt caacaaatga acttgaaaaa gctagaaagc agcttataag caaacagata
     6601 gagagaaaat cattagaaaa attaaaagaa aaaaagtatg gacagtatta taaagaagaa
     6661 aatttaaaag aacaaaacac taatgatgag tttgcaagta tgagttattt aaggaataga
     6721 caagttctat aagaatagta ggaagtgagg atattattgt atgaagatac aagcagattc
     6781 ttataatatt attaaagata ttggggatag aaaaagttca tctaagaaca aaaatataaa
     6841 aaataataaa tttgaagata atctatataa ggcaacagac cataaaaagg ttaaagatag
     6901 aagggaagta agtaagcaaa atattagctc agataaaccg gaaaatagtg ataatgagtg
     6961 tcatttagat gaagataaaa gtaatcactt agaagatgaa aatacatatg aaaaagattg
     7021 tagtaagaat aatgaagctt tgcagcaatt actgaatctt ttaaaggata attataaaaa
     7081 agataaagat ataaacacta tattgaataa tatagaaaaa ctaagtatat cagatgaatt
     7141 aaaacaagaa cttaagctta ataaaaatat attaaataag attgcaaatc aaaattcaga
     7201 gctaaataaa gctaatataa ataactctaa tgatatagaa aatttagaca tagttaaatt
     7261 aagagaactt ttggaagata atgctaataa agatattgaa gtaattacta aaactacaga
     7321 aaatgatacc aaaaatatag atttgtatag ttttgatagc aatagaatgg ataatcttaa
     7381 ctcaaataaa cagaaagatg atagctctga tattttggaa aaactagcag gggtaaatgg
     7441 aaataagagt gggaattttg gacaagtaat aaataaaaat gttgatatga ataaaattaa
     7501 aaatgataat gtcaatttaa aacagataga atcaaatgta atggatgact ctataaaagc
     7561 tattaagcat atgaaaacaa atgatataca agaattgaca attaaattaa gaccaaaaga
     7621 attaggagat atgaatatac aattgttaaa agatggtgaa agtatgagag ctgttgtgac
     7681 tgttttcaat aaagatgtct ttgactctat aaataaaaac atagcagatt taaagcaaca
     7741 tcttgaactt actaatgtaa atataaaaga tgtatcagtg caaatgcaca gcgataatac
     7801 aaatacatct gatacctttg ataaagcatt tgaacaacag aatagacaaa ataaccaaga
     7861 aaacatgagt aagaagaata caaatgataa gcataataat gtagtcatag aagatgacat
     7921 taaagaaaat attattgatg atgatagagt agatttacta gcttagtaag ggggaagtat
     7981 tgcatgtacg gaatagcaac ggctttggca ggaactcagg gaaactatag gtcaaataac
     8041 ttaagccaac ttgaaagaaa tattaatgat attgccaata aacaagttag aagcaaagga
     8101 aatcagacag taacagacaa tgggactcca attataggac aaggtgatga tgctgataaa
     8161 gacttgttta tgaagatact tgtggcacag atgggtaatc aagacccaat gaatccgcaa
     8221 gacccaacag aatatgtgtc acaattagca cagtttgcaa ctttagaaaa gatgtcagct
     8281 atgaatgaga agctagatgt acttttagcc ttaagtaatt ccactctaat aaactctgct
     8341 ttgagtatgg caacatcatt aattggtaaa gaagtagaag tatctgggga agagaaagaa
     8401 gatgaaaaca ttgtaggagt agtaaagtcc acatatataa aagatgggga agtacactta
     8461 gaattagaag tagatggaag tgacaaaact gtaggtgtta aatatgaaaa attacttaaa
     8521 ataggagagg tagatgaaaa ttcaggagaa gaaaatgatt cttcagaaca cgaatctcca
     8581 gaagaagcat aatattaaat gagaaaagag gtaaattcat gataaaagca atgtattcag
     8641 gtgtaagtgg aatgaaagca aatcaaacaa aattagatgt aataggtaac aatgtagcca
     8701 acgctggaac tacttctttt aagaaaagtt cagctagaat aacagattca ttttatcaaa
     8761 ctgtactata tgcaagtgct ccaacagctg cattaggagg aacaaactta ggacaagtag
     8821 gtgtaggtag taagatatca agtataaata aagatatggt acaaggaaat gtacaaccaa
     8881 ctaatagacc atctgattta atgatagatg gagatggata tcttccagta gtaagacaag
     8941 gaacagttat gtatacgaga gatggttctt ttaatttaga tacaggattt aaagcagctg
     9001 atggtgcttt gaagaatatt aaaggtggaa gacttgtaaa tggtgatgga tatatgttac
     9061 aaggtatgat ttatgatggg gaatataaag ctgatactgg agaatttgtt aaaaaaggtg
     9121 atgctaaagg aaaagagcct attgtaatac cacttcaaag tataagtcct aaggatggaa
     9181 cagttcaaaa tgtactagag tataatatat ctaaggatgg aacagtagaa tttttattga
     9241 gtgatggtca aagaactcaa tttgtctatg atatacctga tggtgctaag gtaggagata
     9301 cacctgctgg aatagataaa ttaaaaggta caatacaaaa agtgcaaata tatgcattcc
     9361 aaaatccagc aggcctggat gctgctggag gaaacttgtt taaaccatca gcaaactcag
     9421 gaaacccaca aatagcagga gctacaggca caataataca aggggcaata gaagcttcca
     9481 atgtagatat agcagaagaa tttacagaaa tgataatagc aagtaggtct ttccaagcaa
     9541 acagtaaaat aatatcgaca tcagacgaga tacttcaaga aataataaat cttaagagat
     9601 agtaggtaca tattatgata agagtaacgg atttaaacgg aagaaaatac atattaaatt
     9661 cagaccttat agtgaggatt gattctgtac cagaaagtag gattctcctt acaacaggag
     9721 aaaaaaacct tgtaaaagag tcggtagatg agatagtaga taaagtaata aagtataaaa
     9781 gaaaaataaa tgtaggttat attaggagcg aaaaataatg aaaaaaacag attcactgac
     9841 atttattgga ctaatagtat ctacagtatt agttttagtt ggtgccgcaa aaggaagtag
     9901 ttcagggtta aagaattttt ttgatgtttc atcaatatta atcacagtgc ttggttcatt
     9961 tggagctcta atgattacat ttactataga tgatataaaa cttataaaaa atgcattaca
    10021 atactcattt aagacaatga gtgtatcaaa gttagattta ctagaacaat ttaaaacact
    10081 ttctaaaaaa gctagaaaag aaggtctttt atcaatagag agtgatgtaa tgggaataga
    10141 agatccattt atgaagaaag ggttagagct gtgtattgat ggattggaaa tcaatgagat
    10201 aaggtccatc ttagaactag agatagattc tgttgaagat acaaacaaca gatactctaa
    10261 actatttaaa acttggggaa cctttgcacc tgcttttggg atgttgggaa cactaatagg
    10321 tttaatacaa atgttagctg accttacatc tccagattta atagcatctg gtatgggaaa
    10381 ggcattaatt acaacgtttt atggttcttt acttgccaat atcgctttga acccaattgc
    10441 atataatata gatgagaaga ctgaaaaaga aatttatgta aaggaaatga tgttggaagg
    10501 tataataagc atacaatctg gggagagcag tatagtagtt gaggaacgtt tagccacata
    10561 cctttcaaac aatgagaagt tagaaattat gaaatccaat aaaaatacag aaagagcaat
    10621 gagcaatggc gcgtaaaaaa aataagaaaa aggatgagat aaatccagat gcatggctgg
    10681 ctacttatgc agatactata acactgattc ttacattttt cgtattattg tatgcaacat
    10741 cagttgtaga tatggaaaaa tttaaaaaca tagcagatgc gttaaaaggt cagttttctg
    10801 ggacaagtat atttagtacg ggttcaagta caagtgagaa atctccaata gacagtgttt
    10861 tagtggataa aaatgataaa tcaaatttag aacaggattt aaaagataaa gttaataatt
    10921 ctaacttaca aaatagtgta acagtcaaag atgatagtag agggatttta ctagagttag
    10981 atgattcaat tttatttgac tctgggattt ctgagttaaa agtagatagt aaagctactc
    11041 ttaataaagt atatgacatg ataaagatga tgaaaaataa gatagttata gaaggccata
    11101 ctgataatgt acctgtaaaa tcaagtacac atgaatccaa ttgggattta tcttctagta
    11161 gagcagtaag tgttgtaaga tactttgtag aagaaaaggg catggaccct agactatttt
    11221 cggcaacagg atgtggagag tatcaacctt tggtttcaaa tgaaacagca aagggaaggg
    11281 ctgagaatag aagagttaat atacttataa ctattgacta ggaggaagag gtttgttcaa
    11341 aaataaaaaa attattataa ttattttgat actgattatt ttctcagcgg gagcaggttt
    11401 ttttatatta ggagaaggtg gaaataaaca agttgacaag attgcaggtt tgtttattcc
    11461 aacaggaaaa ggtaaagatg aagtaaagac cgaaaatctt gacttagaag agatggtatt
    11521 aaagctagct gatgaaggtg taagatattt aaaagtatca ataactgtat cttatgattc
    11581 ttcatataaa gatatagaaa aaaatacgcc tttaattagg gataatatta taaattgttt
    11641 tatggcaaaa agggcagatg attttactgc tgataattta aagaatataa aagaagaaat
    11701 taaaaataca ttaaatatac aactaggtga aaatgtaatt caaaatatat atttcacaaa
    11761 tatagtagtg caataaaagg atggtaagac atgatacttt atttactaaa aatattaata
    11821 tgtatcccaa taattttaat attgatagtc ttatctttaa aaattagcca aggaacttta
    11881 ttaaaaaaat ataatgataa atatgtaaaa atactagata ttgtaaatat aagtaaaaac
    11941 aatagcataa taattttaaa aataggtgaa gaaggatgca ttatggcatc ttcttcttct
    12001 agcatgagta aaataaaaga tttgaccaaa gaagaaatat taaaaataga aaacgacatt
    12061 aaagaaagaa attttaaaat tcaagatttt aaaatggaaa gaattaaaaa gaaatatgat
    12121 ttcagtagat atattggaaa atttacaaaa aaggaagata agtaaatgga actattatca
    12181 aaattacctt tggctgatgt accatataca agttcaatgc aattgttttt atttctaact
    12241 gcattaatgt ttttaccatt tgtaatgttc atgatgacaa gctttgtcag aatagttata
    12301 agtttatcat ttttgaaatc agcattagga gcacagcaag caattccaag tcaaattttg
    12361 gtaggacttg caatagtatt gacaatattt ataatgagac cagtattaaa tgagataaac
    12421 gaaaaggcat tacaaccata tatgaaagaa gaagttacta tggaagaagc tatgaaggaa
    12481 gcagaaggac ctattaaaga gtttttatta acacaaacca gacagacaga tttagattta
    12541 tttgtagaac aggctggttt aaaagaaaag aaattaactc gtgaaaatat accactatct
    12601 gttgtagttc cagcatttgc tataagcgaa ctaaaaactg cttttcaaat aggtttttta
    12661 atatatatac catttttgat aatagacctt gttgtggcaa gtgttttgat gtctatgggt
    12721 atgtttatgt tgcctccagt catgatatca ttaccattta agttattatt atttgtaatg
    12781 gtagatggat ggaacttgat agtaaaaaca ttgatattgg gatttggata ggagaaaaat
    12841 agtatgagtg aatttatgat aatgagtgtt cttaaaaatg gcatgtttac aggagctatg
    12901 attgcagctc cagtactcat tgtttctctt gtagttgggc ttttaataag tgtgtttcaa
    12961 gctacaactc aaatccagga gcaaactttg acttttgtac caaaactaat agctattctt
    13021 ttagtagtag ctattggtgg aagctggatg ttacatactc ttattagctt tacttctgag
    13081 atattcaata tgatatctct tataactaaa taagtaggat acatatgatt tttgtattta
    13141 ttagaatagt agcattcttt ggaacactaa gaatactatt tccagctggt acacctgctt
    13201 tatttaagtc actctttgca atcattatat caatattgat ttcaagtact atgcaaattg
    13261 aatatgcaac aaatatagac aacattgtat tgtttacctt atatggtgta aatgagacca
    13321 taactgggat tatgcttgaa tatataacca acctatgttt ttatagtatc agaatggcgg
    13381 gttctatgat ggaccaacag atgggattat caatgattaa tatgtttgac ccaaattcaa
    13441 tgactcagac aactttaatt gataatctta tgaactggac agcacttatg atatttttta
    13501 gcatggatgg tcatcatgtt ttaattagag gtataaggta tagttttgag ttaattccaa
    13561 taggaaaacc atttgtagat aataacatag attatataat aaacatattt gtgcagtgct
    13621 ttttaactgg attcaaaata gcaataccta ttgtactttg tctgcttatg gcagatttta
    13681 ttttagggct tatatcaaga agtgtacctc aattaaatgt aatgatagtt ggtatgccat
    13741 taaaaatatt ggttggtatt gcattattta tcatatcaat acctttaatt gtcaatcaaa
    13801 taagtcattt attaagtcaa ataccaaaga tgtatgaggg aacttttgca ctagctccta
    13861 tgttttttat gggttcagca gataaaacag aagaagctac tccaaagaaa aagggagaac
    13921 aaagaaagaa aggtaatata gcaaagagta gagaattacc cgttgcaatg actctacttg
    13981 cattcacctt attagtacct acattatttt catatgtagt agatacactt aaatcttcgc
    14041 ttaattattt tttgagtcta gatttttata tgaacataaa ttattcaaac ctagagaaat
    14101 tagttatagc aggattaatg gattttttta agatattttt accaatagct attccctttt
    14161 tagttttagg tataatagca aacctatttc aggtgggaat actgtttact ggtgaaactt
    14221 taaaaccaaa tttatcaaaa ttaaatccag ttagtggatt caaaaacatg ttttctatga
    14281 gaagtttgtc aaccttaata aaggatacag cgattatatc tattttggct tacataggat
    14341 atacattctt ccaagataat tatttagata tattaaagtt aggtaacata tacctaccaa
    14401 ctttaatgta cacagttaaa gacttagtat attcaatatt gagtaagatt tgtgtagcta
    14461 tgatagctat agcagttgca gattatgttt atcaaagata cagtcataaa aagcaactga
    14521 gaatgacaaa acaagaagtt aaagatgaat ataaaaactc agaaggagac ccagaagtaa
    14581 aagctaagat aaaacaaaaa caaagacaaa tttcgtctca aaggacaatg caagcagtac
    14641 caagtgctac tgtaatagta accaatccaa cccacttatc tatagcagtg agatatgaaa
    14701 aagggaaaga ccaagctcca gtagtggtgg caaaaggtgc agattattta gcttttaaaa
    14761 taagggaaat tgcaaaggga aatgacatcc ctataataga aaacaaacct attgctaggt
    14821 tgttatataa gcaagtagaa atagaccaag aaattccaga ggacatgtat caagcttttg
    14881 cagagatatt agtggcagta tataaaatca aaaatagata caaagtcccc aaaaggtagg
    14941 ataagtaatg aataagttta atttaaagga aaatatggat gtaatagtgg catttggtgt
    15001 tgtagggatt gttcttatga tgatagtgcc attacctaca tttatattag atattttact
    15061 ggcaattaac ataagtttat ctgtgctaat tttactaatg acattattta caacaaatgt
    15121 acttgattta tctatatttc caacagtgtt acttgtgaca acactattta gattgggatt
    15181 aaatctttca tcaactagat tgattttaac acagggatat gcaggtgaag ttatagaggc
    15241 atttggaagt tttgttgtag gtggaaatta cgtagtagga ataattatat tcttaattat
    15301 attaattgtt caatttgttg ttataaccaa tggttctgga agagtatctg aagtaacagc
    15361 tagatttacg ttagatgcca tgccaggtaa gcagatgagt atagatgcag atttaaatgc
    15421 aggagttata gatgacaaaa ctgctagaaa aagaagaaaa gagcttcaac aagaagcaga
    15481 tttttatggt tctatggatg gagcaaataa gtttgtaaag ggagatgcca tagcaggtct
    15541 tatagtaaca gcaataaaca taataggtgg aattgtaata ggagctgtaa tgctgaatat
    15601 gactcttatg ggtgcagcgc agacatatgt aagacttact ataggggatg gtcttgtaag
    15661 tcagatacct gctctattaa tatctacatc agcaggtata atcgtaacaa gagcttctac
    15721 tgatgaaaac tttggtggat tagtaggtaa acagttaacg gcaataccaa aagcagtaat
    15781 gatgactggt tgtgttctaa tcgtacttgg tatgatgcct ggattaccta aaccagcatt
    15841 tttttcacta ggtataggag ctatagcagc aggttacttt ttaggaagag aaaagaaaga
    15901 tactttagaa gacttggagt ttgaaaaacc tgatatggta aacccagata aaaatgaagt
    15961 agaagatgta acaaacttaa taaatgtaga atcaatagaa gtagaaattg gatatggttt
    16021 aatatcttta gctgatgaat cagcaggagg agatttactt cagagaatag catcaataag
    16081 aagacaatgt gcaatagaca tgggtatagt cgtacaaccc ataagaataa gagataactt
    16141 acagttaaat ccaaaccaat acaatataaa aataaaagga aatgtagttg gtgggtatga
    16201 gataatgcct aatatggttt tatgtatgga tcctatgaat caaggattta gtatatttgg
    16261 aataaagaca attgaaccta cttttggaat agaggcactt tggatagaaa gtgacaaggt
    16321 agaagatgca gagttaaagg gttatacagt tgtagattca tccacaatac ttataactca
    16381 cttactagat ataataaaat caaatgcaca cgaattactg ggaagacaag aagttaagac
    16441 tattatagat gcagcaagag aaaattatag tgctgtagta gatgaattga taccagattt
    16501 gatgacactt ggtgaaattc aaaaagtact taaaaatctg ttaagagaaa aagtaaatat
    16561 aaaggataga gttacaatac ttgaaacgct tgcagaccag tctagaaata caaaagatat
    16621 agagcttctt actgaatatg taagaatagc cttggctaga agtatttgta ataatttagt
    16681 tgatgatgat aagacaattg tagtggctac attgtctcca gaaactgaag agttggtatc
    16741 taataattta cagaggtctg taaatggaac ttatccagca gttgacccag aaaatacaaa
    16801 taaaatattt gaaagtatac aagaagtaat gagcaatgta tatttcaatc ataatatacc
    16861 agtaatagta gtatcaccaa agataagagc tccatttaga aaattagttg aaattgtata
    16921 tcctaatttg actgtattat cattaaatga aatacctaac gatattaaaa tcaaagcaga
    16981 aggtgtggtg agcatatatg atgacgaaaa agtatacagc taacacaatt caggatgcta
    17041 tgaatctagc caaaatggaa ttgggtgaca atattacatt aatagataaa aaagaggtaa
    17101 gaaagtctgg aatacaaggt atcttttcaa aaaaagatat tgaactaact attggatggg
    17161 aaaagagaga aaatgtagaa caaaaagatt taaaacgaga gatagaacaa ttaaagtcaa
    17221 ttattaacaa tatggggttt gataataaga atgataatga tatagataaa atatgtaaaa
    17281 atctactaag tttagaattg aatgaagaaa ttgtagaatc tataagaatt gatttgcaag
    17341 aaatgaagtt caatggaatc gatacaagta aaaatttagt agaaatttta aagaaaaaga
    17401 ttaaaataga aaatcaagta ataaatggta aaattgctct agtcggacca ccaggagtag
    17461 gtaaaacgac tacaatagca aagttagccg caaaattagt ttttgaagaa aataaaaaag
    17521 taggagtaat aactatagat acatacagga ttggtgctgt agaacagttg aagatatata
    17581 ctgatataat gaatattccc tttaaagggg taataagtcc tgatgaaatg gaattggcac
    17641 ttgacgagat gaaagattgt gatgttgtat taatagatac aacaggtaga ggctacaaaa
    17701 actctatgca aatactagaa atcaaaaatc ttatagataa agctgaaact gataatatac
    17761 atttagtttt gaattgtaca acaagagaaa gtgatacaaa agcaattata gattcatata
    17821 gaaatgtgaa ttttaaaagc ttaataatta caaagctaga tgaaacaata acatatggct
    17881 caatatttaa cataatgaat tatgcgcaaa aaccaatatc atatattaca acaggacaaa
    17941 atgttcctga tgatattata aaacctaatg aagataagat tataaggtta ctattaggag
    18001 ttgaaagtat atgatagacc aagctcaaat tttgagaaaa atagcaatgg aaaaaagagg
    18061 acttgatgag tttatagtag agaatgagaa tgtacctaag ataattacaa tagcttctgg
    18121 taaaggcggt gttggaaaga gtaatctcgc tacaaatttg tctatatgct tgaccaaact
    18181 taataaaaaa gttcttatat tagatgcaga cataggaatg tctaatatag atataataat
    18241 gggggtaaat gtaaaaggga ctataattga tgtaattaat ggcgaaaaaa atatagaaga
    18301 tataatttca cagactaagt atggtgtaaa tataatatct ggaggttctg cccttaatca
    18361 tatagaagac tttacagaag ctcaaagaaa taagtttata catagcatag aacaaattca
    18421 cgatgtggat tttataataa ttgatacagg agctggtatg agcaaaagcc tattgtcatt
    18481 tatatactgt agtactgaat ttttcttaat aacaactcca gaaccgacat cattgactga
    18541 cgcatatagt ttattaaaag ctataagtaa ttttggcata aagagaagtg caaatataat
    18601 aatcaatagg gtaattgatt tagaagaagc aaaatctaca tacaaaagaa taaaaatggt
    18661 tgtggacaaa tttctaaacc ttaatctaga attgtatgga tatataatag atgacaaaaa
    18721 agtaagttta tgtgtaaaaa aacagatacc attcatttta gaatatccta caagtattgc
    18781 atcaaaatgt attatagcca tagcaaagag aatggtaagt cagtcaagag aagatattgt
    18841 tgataataaa agttttctaa gaaagatgtt tagtatattt aaaagctaag gaggcgtagt
    18901 taatgaatag agaagaatta ataaaagaga atatgcctct tgtaaagagt atagcaagaa
    18961 aattttttat accagggaaa ggttttgaat atcaagactt agtaaatagt ggagtcattg
    19021 gattgattga tgcaataaat aaatttgatg ctgaaaaagg ggcaaagttt tctagttact
    19081 cttatataaa aataaaatct gctatattag atgaaattag aaatcaaagt ccaatttcta
    19141 aacataatct aactaaagta aataagtata atagagtggt agaaaagttg caatctaaat
    19201 tattaagaga gccaacttca tatgaaattg caaaagaatt aaaggtttca gaaaaagaac
    19261 ttcatgatat cgaaagtaat atagatatgt taaatattgt atcattaaac tatgtaatct
    19321 ttgaagatac aaatgaaact gtacaagatg taataagtga tagagaagag gaagctccag
    19381 aaaatataat agaagaagaa gaaaagttag agattctatc caaagcaatt agtaatttaa
    19441 atgaaagaga aaaattaata ctttcactgt actattatga agatttaaat ctaaaggaga
    19501 tagggaaagt gctaggtgtt tcagaatcaa gagtttcaca actccacaga aaatcaataa
    19561 gaaacttaag aaacaagata aaagaactta aatattctat atagatggtg attgataaga
    19621 tgatacaaat tatattaata gtagtgtcta ttttaataat agtaactata gttataaatt
    19681 tacataagga atcaaaaaat aaagataaca gatactttga taaaatattt gagcaagaat
    19741 atgaagcaaa tgatagatta ataattgcag aaaaaagaag attcttttta gacaaagtac
    19801 ttgcagaaaa aagaattaat agtcatataa aagtttatga aaattataac ctaataaagg
    19861 ctaatgaaga aagaaaggaa aacatattta tagaatcaaa aaaggaagta agacaagaaa
    19921 atgagttatc tcttaaagtg aaacatctta taagttcagg ttatgatgat gtggaaatat
    19981 gtaagatgct agatattgga aagggggaac tgttattaat aaggagttgt tacaaaatat
    20041 aaggatatta ttatgtgata aaaaagtatt cttaggaata ggtattggta tattaatatc
    20101 aactttatgt ataatgcttt ttagtaataa tgatatgagt aaggcagaga tagaaaaaaa
    20161 ggccagagcg ttaggcatga aatatacaag tgagatgaaa gttttagaat agatgatatt
    20221 gtttagacat ttacaaaaaa ggaggtcata atgtgataag aggattatat acagcagttt
    20281 ctgctatgat aactaatcaa caaaagcaaa atgttgttgt caataaccta gctaatatgg
    20341 atacaaatgg atataaaagt aaacaacttt tgactaaaag ttttgatgaa ttaaagttgg
    20401 agggttacaa taactacgca aatggaacta aacaaaagca gattataggc gatataagtc
    20461 caggtatttc tatggatgaa actatcacaa attttaatca aggacctata aaaactactg
    20521 ataataaaat ggatgtagct attcaaggta agggattttt tctagttagt gatgcagctg
    20581 gaaatcaatt ttacactaga aatgggaact tcagaacaga caatcaaggt gaccttataa
    20641 caagtgaagg gtattatgta cttgggacga atactgctac tggtgctgta ggtcatataa
    20701 atgtaggaaa tcaaaaattt gaagtggcaa aagataatac aatatcacta gacggaaatg
    20761 ctatgtataa atttaatatt gtagatttta ataactacaa taacttaaaa aaagaaggag
    20821 ataacttata ttctggagga ggagctatga aagtaaataa tgcacttaca atacaaagtg
    20881 cgatagagtc ctctaatgta gatatggtat ctgaaattaa taatatgatg acaatatcaa
    20941 gagaatatga ggcaaatcaa aagataattc aggctatgga ttctaaatta gcaaaaatag
    21001 caagtgagat tggttcagta agataggtaa taggaggtga atttttatgt acacaatgtt
    21061 ttatactagt aaaacagctt tatcagcaaa tcaaagtaaa ctagatataa tatctaacaa
    21121 tatagcaaat tctgattcaa caggatataa aaaaataaat atggaatatt ctgatttagt
    21181 ggatgaagta ctaaatagac catcatatcc tacaaatggt aaagatatat cgactggaac
    21241 aggagctaag gcaacagctc ccatgagaaa ttatgaacaa ggtgcattgg ctcctacaga
    21301 tagtaagagt aatttggcga tagatggaga aggattcttt agagtgataa gaaaagatgg
    21361 tacgtatgca tatacaagaa atggtggatt taatgtagat gctttaggta agattgtaga
    21421 tgataatgga aatatattag atgtccaatt tgatgcagga tataactata ataatacaaa
    21481 tatagatagt acaaacctga cagtttcaag aaatggagag ctattcgtag ataataaaaa
    21541 aataggaaaa attaacttat atcaacctat aggaactcaa aattttatat ctgaaggaga
    21601 tagtttattt gtcgcaactg atgcagcaca gattaaacaa gttgagaaag tcgatatagt
    21661 tcaaggctat agagagaggt caaatgtttc tttacaagaa gaatttgttg acttaatagc
    21721 cactcaaaga gcatttcaaa tgaactcaaa gggaataaag acagcagatg aaatgtggca
    21781 aatagcgaat aatttaagag ctaaataatt atgtaaggtg aggaatattc atgaatgata
    21841 tgaaagagta cgattttggc aaacctcaga gttattcaag agaaaattta acttgtttaa
    21901 attttttatt agctgaattt tgtaaaaaat ataaaaacta tataatttat gaacttaagt
    21961 gtaattctag tatggttgtt gatagtatag accaagtaaa ttatcagact tttttagata
    22021 gagtaacttc ttcatgcgta gtagtacaaa atgcaatgga acctgttatg aaaaactttt
    22081 cttttagaat agatagaagt gtagctgata tgtggattga tataacttct ggaggaactg
    22141 gagttgtaag agataatgat agagagctta cagaacttga taaaaaagtg cttttacacc
    22201 ttatggatga tttagtaaga aatatgcact tatttgaggg ttttgaaaat atacaagtat
    22261 tggatacaca tacacatatt aatttaccac aattatgttc tccaactgca ccagtatgcg
    22321 ttgtagattt aaaagtgcta aatgataatg agtatattgg aagtgtttct ctttgttttc
    22381 catacaatgg tttagaagct ctgttagaaa gtatatctgt tatagaattt tttgataatg
    22441 atataggaga tgattcagag gaatttacca aaaaaatata taatagtgta tctaatatag
    22501 aattatcggt tattgcagag ataggaaaag tatcaattaa tgttgaagac ttacttaaac
    22561 tagaagttgg agatgtaata gtaacaaata aaaaaataaa tgattatata gatatatttg
    22621 tagaaaattc aaaatcatat acagcaacac caggatttat atcaagtaaa aaaggtgtaa
    22681 aaataaatga tgctgttgga aaagaggtgt agttatggac aatagtaatc ttatttcaga
    22741 tgaagaagca aagatgctat taaatgataa agttgaaagt gaagttgatg gagatgatga
    22801 gttagcctta gatgtgtctg aagttaatcg aaataatata caaaggatac ttgaccttaa
    22861 gttagaatta tctgtggtta taggaagaac taagatgact ctaagagata ttttaaacct
    22921 acataaaggt tctcttattg aactggatac tttagcagac caagatgtgg aaatactaat
    22981 agataataaa gtgttggctt atggaaaagt agtggtggta gatttaaatt ttggtgtaaa
    23041 gataacaagt atagtaagtg aagaagatat ggtaaaatct cttgtttaga gataacatca
    23101 attaatactt aaggcatgag gtggtactta ttttaaactt aaatgatata aaaaaagata
    23161 tattaaaact caataaaaaa gactcatgtt ctctatataa tgaatctatt aataatataa
    23221 ataataattg tatatcgagt ggattgaaat ttttaaaaca agcaagagat ttaaacccag
    23281 atgatgttga tatattaaat ctcacaggtc ttgtaaattt actaaaatgt aattttgatg
    23341 aggctgtaga aagtttctat aagagttcac tttgtgaaaa aactgctctg tgtgaaaagt
    23401 atataaacat gcttacatct gaggagtttt taattttctt tgaaaggtac aatcaactca
    23461 taaggttaat aaacagagga atgtataaag aagctataca agggtttaaa cttataactg
    23521 agcaatactg tgatttaata gaaccttatg aattgcttgc cttactgtat gacaaggaag
    23581 aagaatatat aaaatttgat gaatgtttag aagtattgaa gattattgat aaggaaaatt
    23641 atttattaaa tcaagaaaat aacacattag atatgtataa tagtgctaat atacaaacta
    23701 aaagtaatat aaaagtagat gattataaga atgagttctt acataaaagt agtagtaacc
    23761 ataaaattaa tgattataat gatgtaacta aagaaaagag ttcaagcaaa aaccagaaaa
    23821 gttcagattc taataagaaa aaaagtatta aaaaaaataa atacctatat atagccataa
    23881 tattaggaat attattagta ggacaaagta tatatagttc ttataagatg aataatttaa
    23941 ctgataaaat atctaaaaat aatgaaacca aaaatgaaga gtcaatagat tctaacttga
    24001 ctaagaaaga aaataatata gaagaaaatg aagatatgag taaggcttct aatataaata
    24061 agagtaataa aaacattaat aaagaggatg atgtaaaatc taaaaaaaat actgtagaaa
    24121 atgaagatga taaagattct gacttattta cagaggatga attgatgtct aaggcgaata
    24181 ctttaaaatc agaaaagaaa actaaatctt ctatacaact atataagaaa gttgcagata
    24241 taggtgaaaa taaagggaat acatctgaag caacttatca agttgctatt ctaagtgaaa
    24301 agttaaaaga ctataaaact gctgaagaat attataaaat gtatgtagaa aattactcag
    24361 aaaaagatgc ttattttgat gaatcttatt ataatttagg tatgatgtac tataacaatg
    24421 gtgatttaaa aaattctaaa ttgacattaa agaagttggt taataaggta ccaaatagta
    24481 tgtataataa ttcaaaagta aaagaaattt taaaagaaga atagttaaat tagataagaa
    24541 aaataaat
//
LOCUS       DACXMZ010000035        24473 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.035, whole genome shotgun sequence.
ACCESSION   DACXMZ010000035 DACXMZ010000000
VERSION     DACXMZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.035"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="JD016_05595"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="JD016_05595"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            275..466
                     /locus_tag="JD016_05600"
     CDS             275..466
                     /locus_tag="JD016_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAU4999107.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            621..2030
                     /locus_tag="JD016_05605"
     CDS             621..2030
                     /locus_tag="JD016_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAU4999108.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            2161..2850
                     /locus_tag="JD016_05610"
     CDS             2161..2850
                     /locus_tag="JD016_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAU4999109.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2853..3788
                     /locus_tag="JD016_05615"
     CDS             2853..3788
                     /locus_tag="JD016_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAU4999110.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3785..4678
                     /locus_tag="JD016_05620"
     CDS             3785..4678
                     /locus_tag="JD016_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAU4999111.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4756..5502
                     /locus_tag="JD016_05625"
     CDS             4756..5502
                     /locus_tag="JD016_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAU4999112.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5503..6336
                     /gene="rsmI"
                     /locus_tag="JD016_05630"
     CDS             5503..6336
                     /gene="rsmI"
                     /locus_tag="JD016_05630"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAU4999113.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6359..7063
                     /locus_tag="JD016_05635"
     CDS             6359..7063
                     /locus_tag="JD016_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAU4999114.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLKTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7251..7679)
                     /locus_tag="JD016_05640"
     CDS             complement(7251..7679)
                     /locus_tag="JD016_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAU4999115.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7716..9146)
                     /locus_tag="JD016_05645"
     CDS             complement(7716..9146)
                     /locus_tag="JD016_05645"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAU4999116.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9278..9862
                     /locus_tag="JD016_05650"
     CDS             9278..9862
                     /locus_tag="JD016_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HAU4999117.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9878..10399
                     /locus_tag="JD016_05655"
     CDS             9878..10399
                     /locus_tag="JD016_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HAU4999118.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10465..10711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10795..12732
                     /gene="metG"
                     /locus_tag="JD016_05660"
     CDS             10795..12732
                     /gene="metG"
                     /locus_tag="JD016_05660"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAU4999119.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12762..13532
                     /locus_tag="JD016_05665"
     CDS             12762..13532
                     /locus_tag="JD016_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family deoxyribonuclease"
                     /protein_id="HAU4999120.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13870..14301
                     /gene="phnG"
                     /locus_tag="JD016_05670"
     CDS             13870..14301
                     /gene="phnG"
                     /locus_tag="JD016_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAU4999121.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14316..14891
                     /gene="phnH"
                     /locus_tag="JD016_05675"
     CDS             14316..14891
                     /gene="phnH"
                     /locus_tag="JD016_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAU4999122.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14894..15979
                     /locus_tag="JD016_05680"
     CDS             14894..15979
                     /locus_tag="JD016_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HAU4999123.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15999..16868
                     /locus_tag="JD016_05685"
     CDS             15999..16868
                     /locus_tag="JD016_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAU4999124.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     DPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16861..17727
                     /locus_tag="JD016_05690"
     CDS             16861..17727
                     /locus_tag="JD016_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAU4999125.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17768..18481
                     /locus_tag="JD016_05695"
     CDS             17768..18481
                     /locus_tag="JD016_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAU4999126.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18482..19651
                     /locus_tag="JD016_05700"
     CDS             18482..19651
                     /locus_tag="JD016_05700"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAU4999127.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19655..20491
                     /locus_tag="JD016_05705"
     CDS             19655..20491
                     /locus_tag="JD016_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAU4999128.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20492..21487
                     /locus_tag="JD016_05710"
     CDS             20492..21487
                     /locus_tag="JD016_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAU4999129.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21505..23193
                     /locus_tag="JD016_05715"
     CDS             21505..23193
                     /locus_tag="JD016_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HAU4999130.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23205..24272
                     /locus_tag="JD016_05720"
     CDS             23205..24272
                     /locus_tag="JD016_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAU4999131.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9755 a         2748 c         4378 g         7592 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgtaattt tttttatttt ttgtcaaaaa
      181 aaacaaaaaa tacgtatttc ttttttcatt tcgggaaaac taaacacagg aattatggac
      241 tttataaaaa tttaattctt gaggagtgat ataagtgaaa aaagttgctt taattttagc
      301 tataataggt gcgcttaact ggggtgcaat tggtattcta ggtacagact taataggtag
      361 tatctttggt ggaacatatg aaatggtaag tagaataata tacttcattg taggtttagc
      421 tggattatac ttaataccaa gtttaatgtc tgatgataga gaataattta tgcttttatg
      481 ttaaatacct cattctaaat gtttagaatg aggtatttaa ttttataaaa atattaattt
      541 gtttcttttt taatggtaaa atagataaga attgaagttt taaaaagaag tattatgtaa
      601 aaaaggagta ctattaatat atgtttatta ttaatgaatt aaataagatt gtaaatgaag
      661 aagtaatatc ttttcatatg cctggtcata aaaagggaca ggtttataaa aagttaggat
      721 ataaagatat actaaaaaat ttgtataaaa tggatacaac tgagatatta ggaacagata
      781 atttacactt tccggaagga attataaaaa attcacaaaa aaaagcatca agagtattta
      841 aaagtgatta tacttgctat ttagtaaatg gtagtagctg tggaatacaa tctgcaataa
      901 tgtcagtatg tagtcctaaa agtaaaataa taacaaatag agactgtcat cagtctgtta
      961 taaatggatg tattttagga gatatagata tagagtatgt gccttgtgag ataagtgaag
     1021 agacaaatgt cttaaaaggg atatctataa gtaatgtgat tgatataata gataaaaact
     1081 tggatgcaaa agcagtactg cttacttatc ctacatatta tggaatgaca tatgatttag
     1141 aatacatatg taattatgca catagcaaaa agatggtagt gatagtagat gaagcacatg
     1201 gtgcgcattt agggcttagc gagaggcttc ctaaaacagc tcttgagcaa ggcgcagata
     1261 tagttgtaca gagtacacat aaaactcttc cagcatttac acaatcgtca atgatacata
     1321 tacagggaga ccgagttgat atagataaaa tattgaacat gttaagaatt acagaatctt
     1381 caagcccttc atatttatta ttatcttctc tggaattagc agtagatata tatgaaaata
     1441 aaggaaaaga attaatggaa gagcttttaa ataatattat aactttcaaa aataaagttg
     1501 attcaaatat taatatatac gacacaaatg ataaaacaaa aatatttata tcatctaaaa
     1561 atatgggttt aacaggttat gaactagaaa atattttaag aaacaaatat aacatacaag
     1621 tggagttatc taattattat ggagttcttt taatatgtac aataggaaat actgttgagg
     1681 atttctctag tttagaaaca gcattaaaca atattgtaaa ggaagacttt aaatttgaaa
     1741 agttagaaaa taaaaaatac cctgttgtta ttccaaagaa aatattaaca cctagagaag
     1801 cattttataa aactaaaaaa agtgttaaaa tatatgacag cataggtaaa gtttgtggtg
     1861 aatgtatagt tccataccca cctggaataa gtgtaatatc tcctggtgaa gtaataagta
     1921 aagaaataat agactatcta atgttttgtc attctaaagg catgattgtg agtggattga
     1981 aagatgtaga tttaaatttt atacaaataa tagatttaga agacatttaa aaattatgat
     2041 ataaaagtag atagaagtag atagtttaaa attgtgaaga gaaataaata gactgcttat
     2101 atagagctat ctaataaaag tactaaaaaa gtaaaaagtg tttgtatgga ggatatgaaa
     2161 atgaaaggta agttaataat tatagagagt ggttcagatg caagtggaaa ggctactcag
     2221 acagaaatat tatatgaaag attaaaaaaa gaaggctata tgattaaaaa ggtagaatat
     2281 ccaaattatg aatctccatc atctgccttg gtgaaaatgt acctaaaagg agactttggt
     2341 aaaaatgcat cagatgtaga cccatatata gcatcaactt tttttacagc tgataggtat
     2401 gcatcattta aaaccgattg ggaggagttt tacaatgaag gtggaataat tatcgcagat
     2461 agatatacaa cctctaatat ggtacatcag gcctctaaaa tggatatttg tgatagagaa
     2521 gagtatttaa attggttagc tgattatgaa tttaatttat ttaagatacc acaacctgat
     2581 tgtgttatat ttttagatgt gcctattgaa tttagtaaaa agcttatgga gaatagaaaa
     2641 aataaaatta caggggaaga aaaaaaggat attcatgaaa gtgacataga gtatttagag
     2701 aaatcttaca acaatgcttt atatatagca gataaatatg attggaaaaa aataaattgt
     2761 gttttagata acaaattaag aagtattgaa agtatacata atgaaattta taaaatagtt
     2821 cttgattcaa taaaacctat ggagagataa atatgtattt tgaaaatatt atagggcaag
     2881 attttgcaaa gaaatattta acaaattcta taaagaaaaa taaacttaat aatgcgtata
     2941 tgtttgaagg aatggatgga ataggaaaaa agaaatttgc agatgaatta tcaaaactat
     3001 tacttgatta tgaaaatctc gaaaatagtc cagactatgt actcataaaa ccagatggaa
     3061 atagtataaa aatagctcaa atcagaaatt tacaatcaga tatagtaata agacctcata
     3121 aagactataa aatatatata atcaataatg ctgaaaaaat gactgtggaa gctcaaaatg
     3181 cacttttaaa gacgctagag gaacctccaa actatgctat aataatctta gtcacaaaca
     3241 acaaggaatc tttattagaa actataaaat ctagatgtga tataataaag ttctcaccta
     3301 taccaattga agacttaaaa cgttatttga taaatacagg tatagaagaa gaaagagctc
     3361 aattattggc tattttttcg agaggaagta ttgagaatgc tctaaatcta tctcagtctt
     3421 ctgagttttc aatgatgaga gaagatatac aacagtatat tcagataatg ttagataaaa
     3481 acatagtgga gattttaaat ataccaaata atatggaaaa atatagaggt aagattatag
     3541 ctttattaga tatgatgata aactattttc gagatatcat cctattaaaa gaaaatgtaa
     3601 acaagaacat gttaataaat gtagataagc tagttttcat tcaaaatatg agtggaaaaa
     3661 ttagttattc tcaattgtcc aagattattg atataataga agatgcaaag agtaaaataa
     3721 aaagcaattg caatttcaat ataagcatac aagttatgtc tttaaatata tacgaggtga
     3781 ttaaatgata aagatagtag gtataaggtt taaaagtgct gggaaaatat attattttga
     3841 ccctgttgat tttaatatag agcaagatat ggatgtagtt gtagaaactg caagaggctt
     3901 ggagtatggg aaagtagtcg ttggtccaaa agatatggat gaaagcgaat tagtttctcc
     3961 tttgaagcca attataagaa tagctacaga agaagataaa aaagtatatc tagaaaataa
     4021 ggaaaaggca aaagaaactt ttgagttgtg ccaacaaaaa ataaaagaac atgagttaac
     4081 aatgttttta atagattgtg agtatacatt tgataggaat aagttgattt tttactttac
     4141 agcagaagga agaatagatt ttagagaatt ggtaaaagac ttagctgcta tatttaaaac
     4201 tagaatagaa cttagacaaa taggtgtcag agatgaagct aaatctatag gaggtctagg
     4261 gccttgtggt agaaaacttt gttgttcatc ttggttaggt gattttcaac cagtttcaat
     4321 aaaaatggca aaagaccaaa gtctatctct aaatccaaca aaaatatctg gaatatgtgg
     4381 gagattattc tgttgcttaa aatatgagca tgatgtatat agtgaagcaa tagaaaaaat
     4441 gcctgttgta ggtgcattgg tgcaatcaga tgatggaaaa ggaaaagttg tagaagtgaa
     4501 tcctttatta gaacaaatta aagtggaatt tcaagacaag acagtaaaga tatacacaag
     4561 agaagatata aaaatactta gagagcctaa aaaatgtgat ggctgtggtg gtaaatgtaa
     4621 ggataatgat ggattagatg aagccacact aagagagcta aagaaattag aagattaaat
     4681 ttaaggtagg gcttttccta ccttttttaa agcaattaat gcttaaagtt attaatattt
     4741 gaggagaatt tatatatgca attaaaagat tcagaaagaa tagatgattt acaattaaaa
     4801 ggccttaaaa ttatacaaga tactaatggt ttttgttttg gtatagatgc agttttatta
     4861 gccaatttta ctaaaataaa aaaagatgcc aaagtagtag atttgggtac tggaactgga
     4921 attataccta ttttgatagc tggaaaaagt gaagctaaaa aaattattgg cgtggaaata
     4981 caagaagatg tatatgaaat ggcaactcgt tctataaaat taaatggatt agaagaaaga
     5041 gttgagatta taaatgaaga tataaaatac atagacaaag tcttagacat aaatggttac
     5101 catgtagtca catcaaatcc tccatatatg catattgatg gaataaaaaa tccaaatgat
     5161 aaaaaagcaa tatcaagaca tgaagtaagg tgcaatttgg aagatgtaat tagagctgca
     5221 tccagattgg ttatgcctag aggaaaattc ttcatgatac atagaccaac aagattggtt
     5281 gatataataa ctttagggag aaaatataaa ttagaaccaa aggttattca atttgtgcat
     5341 ccaagacctc aaaaagcacc gaacttagtt ttggttcaat ttgtaaaaga tggaaggcca
     5401 gagcttaaaa tattggaccc attgtatgtt tatggagaag atgggaatta tacaaaagaa
     5461 ttaaaagcaa tatataataa taaagatata ggagagttgt aaatgagcgg gaaattgtac
     5521 atatgtccaa caccaatagg aaacctagaa gatataacat atagaacttt aagaatatta
     5581 aaagaagtag acttgattgc agcagaagat acaagacata gtataaaact tttaaatcat
     5641 tttgaaatct caaaaccact aactagttat catgaacata ataaggattc aaagggagat
     5701 tatttgataa ataaactaat tgatggtgaa aatatagctc tgataagtga tgcaggtatg
     5761 cctggtatat ctgacccagg agaagagata ataaaacaag ctatacaaaa taacatagag
     5821 atagaggtat tgccaggtgc aacagccttt gtaacggctt tagttggctc tggtatggac
     5881 acacatagat ttgtttttga gggcttttta gatagagata aaaaagttag gaaagcacaa
     5941 ttggaagaat taaaggaaga aagtagaact ataattttct atgaatcacc acatagattg
     6001 aaggatacac taaaagatat gcttaaaatt cttggtaata ggaatatttc tataaataga
     6061 gaacttacaa aaaaatatca agaaataata agagaaaata tagaaactac cataaaaata
     6121 tttgaggaaa aagaagtaag aggagagttt gtattaatag tagagggttt tcatggtgag
     6181 aagtcaaaga aagaaaatta tgatgggctt acagataggg aatatgtttt aaaactaatt
     6241 gaaagtggta tgaacaaaaa agatgcaata aaaatagttt gtaaagatag aaagttaaag
     6301 aaagatgtgg tttataaaca agttttagat ttataaaacc tgaatctgag gtgattttat
     6361 ggaaataggg aaaattataa tagagagttt aaagagggaa gatacacata aaatatttat
     6421 agaattagat aataagcatg aattagaaaa aataattcct aaaataagct gtatgaagtc
     6481 tgtaggtgaa tgtaaatatc atgtagtaaa ttgttttgaa cattctataa atgcattaaa
     6541 agaattagaa atagtattaa atgataagga tttttttcca atacatctaa gaaaacatgt
     6601 gtcaaactat ttaaaaacat atattgaaga aggaataaat aagttacatg tattaaagct
     6661 gggaacattt ttacatgata taggtaagcc tgacagtatg accttagatg aaactggaag
     6721 agttcatttc acaaatcatg aaaaaatagg tgctcaaata attgacaata tgggaataaa
     6781 gttagagtta tcaacagaga catatagact tataagtaaa tatgtaagat atcatatgat
     6841 acttttgtct ctatataaga aaaatgattt gagtaggaaa gaattaatta atgtatttaa
     6901 cttagtagat gaggacacta taggtgtaat aatgcttggg tatgcagata tagtgtctac
     6961 agtaaaatta ttgggcaaga gtggagaagt cagtgtgtta aagacatata tggaatatat
     7021 tttaacaaat tacttgtata agagtaatta tacacatgta taaatttagt taatataatt
     7081 gagttttaga ggattaattg aagtttgaaa tatacggatt tcaaaaacat aaaagaagtt
     7141 gtctttgttg taaaattata aaagacaact ttttttatat caaaaattga aattttatta
     7201 aggatactta atggtctatt aattaataaa ccattagtat ttaatttata ttacaatatt
     7261 gattttgctt gtattggcgt tgatagtata acagaggtct tagtagaacc aattttcttt
     7321 atagtatcaa tcacattttc tagctcgtac atatctttta caattacttt taaaagtaag
     7381 caatcatcac ctgttatatg gtgacattct acaatcctag ggtcctttgc agctgactca
     7441 ataaattctg tatatccatt gcttggaaga gaaatatgaa taaatgcctt tataactctg
     7501 cctaatgaat ctgggttgac aatagcttta tatccttcta taacaccaga ctcttctaat
     7561 cttttgactc tttctgaaac tgcaggagaa gttaaaccaa ctatttttcc taagtccttc
     7621 atagaaattc tcccatcatc ctgtaaaatt tctatgattc tgtaatctgt aacatccata
     7681 aataaacagt cccccttaaa taaaaataat taatgctata aaactttttt agaatctatc
     7741 atttgtgttt ttaaatacca aagatttcta gataaatcaa ccttataaat gtgtggtttt
     7801 gaaagtcttt tgtattgttc ccagatagta cttaattcat attgtacatg attttttatt
     7861 tcaagaacag cttttttatt atatttgcat ctgcctttta tatacaatgg tttaagaagc
     7921 tctttaactt tgtaatttgt aaataccttc gttttccatg tataagtagg atggaagatt
     7981 tctaaaggtt tagactcatc tataacttca tcatgtaaca taatcaaatc tgcctctgcc
     8041 ttattgtctt cgttatatat tcttataact ttcttgtaac caggattatt tattttttct
     8101 ggattctcag aaatctttat cttaggttca agaactcctt tttcacaaga tgcagctaat
     8161 ttataaactc ctccaagaga gggtgaatcg aaagatgtaa tgagctttgt acctactccc
     8221 catgaattta tagtagctcc ttctgcttta agtgatgtta tggtatattc gtctaggtca
     8281 ttagatgcag ttatgctgat atctgaaaag ccagcattat ctaattgttt tttagcttct
     8341 ttagataaat aagctaaatc tccagagtca agtcttatac ccataggttt gtatcctttt
     8401 tcagaaatat ttttgaatac ttttattgca ttaggaactc cactgtttaa tacatcataa
     8461 gtatctacaa gtagtaggca tttatctgga taaacatcag catatgcttg gaatgcttca
     8521 agttcattat caaatttttg tacccaactg tgagcttgtg taccaattat aggtatatca
     8581 aacattttcc ctgctagtac gtttgcagtt ccagcacaac caccaactac ggcagcccta
     8641 gccccatata tccctgcatc tggaccttgt gcacgacgta atccaaattc aaatacaggg
     8701 tctccttgag ctgcaaagca aactctagat gctttagtag caataagaga ctgaaaatta
     8761 actattgtta acaatgctgt ttcaataagt tgagcttgat ataaaggtgc cttaacagta
     8821 attaatggct cattagggaa catgatagtt ccttcctcaa cagcatatat atctcctgtg
     8881 aatttaaatt cttttaaaaa ttttaaaaac ttgtcagaaa ataaatttaa gttttttaag
     8941 tactctaaat cttcatctga aaaatgaaga ttatctatgt attcaacaac ctgatcaata
     9001 ccacaaacta tagtataccc tccatcacaa gcgttttttc taaaaaacat atcaaataca
     9061 acaatgtctt catgaatatt cttttcaaaa tagccattta acatagtaag ctgatataaa
     9121 tcagtaagaa gtgttagatt tctcattatt tctctcctta attattatta aatattaaaa
     9181 attgtaaaaa ctcccacact tatactaaaa cataatatag tacaactttc aagtataaag
     9241 tattacctaa ctatcataaa attatttaaa gaggaggatg attataatag gaaaaatatg
     9301 gttttatatg gtatcgatag gaataatagg aagcttgatt tctaaaaata tgggtgaatt
     9361 aaataaagtt atattaagtg aaacatcaaa aggtatagag tttgcaataa gtctagctgg
     9421 tatcatggca ctctggatgg ggataatgaa tatcgcaaaa gaatcaggtt taattgaaaa
     9481 gataggtcaa aaactaaatc ctataatgag aagattattt ccttctattc caaagggtca
     9541 taaagcaatg tcttatatag ttatgaatat tgctttaaat atgttgggtg ctggaaatgg
     9601 tgcaacagca tttggtttaa aagcaatgag tgagcttcaa actttgaata acaagaaaga
     9661 tacagccact aatgatatga ttatgtttat ggtaatcaat atatcatcta tacaaataat
     9721 cccatttacc ataatcaaat taaggatgga tatgggggct caaaatccaa gtgaaataat
     9781 ttttacgact ttatttgcaa ccataatatc tacagcagta gcaataataa catgtaaatt
     9841 ttttcaaaga agatatagat aaaggggatg tagatacatg gagatatttt caaacttact
     9901 tataccaata ataattctgt acatagtagt ttatggtaaa tataaaaaaa tagatgtgta
     9961 tgatagtttt gtaaaaggag caattgatgg attaaaagca gcatgggata tcctaccata
    10021 tataataggt atatttttgg caataggaat attcaaaact ggtaaaggtc ttgatatgtt
    10081 agaatggcta tttacaccaa tagcaaatat gatgagtata ccaaaagaat taataggtct
    10141 aataagtgta aaacctttat ctggaagtgg tgctttaggg atgtacagtg aacttgcaaa
    10201 tagagttgga ataggaagtt tagtagaaaa aatgggtgct acgattgtag gttcttctga
    10261 gactattttt tacacaatgg caatatacta tggaagtctg aagataaaga atactagaca
    10321 tacattatca tgtgctatga ttagccatgt tgcaggtgtc atagcagctg tttttatttg
    10381 ctatgtaata tttgtataat ttgttgacaa tatgggaaaa attttataat atagtgatta
    10441 tatagaaaca aatagtaata caaattacaa gctatgaaaa gaagagtaaa taaaggtttt
    10501 ttctatcaga gagctaggtt aggtgaaagc tagtgtcaaa agctttattg aagatggtct
    10561 tggagctttt tatctgagca agtgtgctag ggtaaaacgg ttggacacgt tatagtctac
    10621 taaggtatct ataaaatgtt tctacatttc atgtgaaaca taactattat agatgaatta
    10681 gggtggtacc gtgaataatc tcgcccctat gttaactcat aggaggcgat tttttattat
    10741 ttaaatgaaa gaatcatatg attacgaacg ataaaaaatt aggaggtaaa taacatgagc
    10801 aaaccgagtt tttatgtaac aacaccaata tactatccaa gtggaggatt acacatagga
    10861 catacttatt caacagtagc tgcagataca atagctagat tcaagcgttt ctgtggatat
    10921 gatgtaaagt tcttaacagg aacagatgag catggtgaaa aaattcaaaa gaaagccata
    10981 gaacaaggaa tgtcagagat agagtatctt gatggaatga taaaagatat aaaggcctta
    11041 tggaatacta tggatatatc ttatgatgat tttataagaa ctacagaaaa aagacataca
    11101 gacataatac aaaagatatt tactaaacta tatgagcaag gtgatatata taaaggtgaa
    11161 tatgaaggta gatactgtac tccttgtgag agtttctgga cagaatctca attactagaa
    11221 ggaaataaat gtcctgactg tggaagagaa acatatttag taaaagaaga atcttatttc
    11281 tttagattat ctaaatatga agacagatta aaagagttat ttaaagatga tagtttctgt
    11341 tttcctgctg ctagaaaaaa tgaaatggtg gcaaactttt tagacaaagg attagaagat
    11401 ttaagcgtaa ctagaacgac ttttgactgg ggtataaaag ttccttttga tgaaaaacat
    11461 gtaatttatg tgtgggtaga tgctttatgt aactatataa cagcattagg ttatatgact
    11521 gataatgatg aagaatttaa aaaatattgg cctgccaatg ttcaaatagt aggtaaagaa
    11581 atagtgagat tccatacaat aatatggcca gcgcttttaa tggctttagg tctagaagtt
    11641 ccaaaacaag tatttggaca tggatggata ctatttgctg atgataaaat gagtaaatca
    11701 aaaggtaatg tggtttatcc agaacctata atagaaagat atggaataga cacacttaaa
    11761 tacttcttat taagagagtt tgcatttggg caagatggaa gttatactca tagaaatttt
    11821 gtaacgagaa taaattatga tttagcaaat gatttaggaa acttaataag tagaactgta
    11881 gctatggttg aaaaatacaa taatggcata ataccaacag caaaagtttc aactaatttt
    11941 gatgctgact taaaagaaca agctgtttca actagagaaa actttgaagc tgaaatggat
    12001 aaaatgcaat tccatgaagc attagagagt gtatggaaat tagttagaag aactaacaaa
    12061 tatatagatg aaactatgcc ttgggcacta gctaaagatg aaactaagaa agatgaatta
    12121 gacacagttt tatataattt atgtgaatct ataagaataa tcgcaacact aataaatcct
    12181 ataatgaatg agactgctaa taaaatatat gaacatatag gaataaaagg tcaagatgat
    12241 ataactacat gggaaagtac aaaaacattt ggtcttattg gagaaaatgt aaaagtattt
    12301 aaaggtgaac ctttattccc aagattagat gtagaaaaag agatagaaga attaactaaa
    12361 atgttctctg ggaaacctcc agtagaagaa aaacctttag agcataaaga agaaattact
    12421 attgatgatt tagacaaaat agagcttaga gtagggaaaa taataagctg tgaaaaacat
    12481 ccaaaagcaa ataaattatt agtatcacaa gttaagattg gaccagaaac aagacaaata
    12541 gtatcaggaa tagctgaata ctataagcca gaggatttag taggaaaaga ggttacagtg
    12601 gtatgtaact taaaaccagt taaattaaga ggtgtagaat ctcaaggtat gatattagca
    12661 gcaggtgatg atggagaccc atatgtttta ccatttacac aaggggctaa agatggatgc
    12721 gaggttcgtt agtaggtaat taaatagtaa ggagattaaa gatgttattt gactcacatg
    12781 cacatttaaa tgatgaaagt tttgatgaag atagagatga acttattggt tcattaaaag
    12841 ataaaggtgt agatttggtt gtaaatccag gagcagatat agagacgtcg ataacagcaa
    12901 ttgaacttgc gaaaaaatac gactttatat attcagcagt tggggtacat ccacatgatg
    12961 tatcaaaact agatgataca gcaatagaaa ctctaagaaa acttgcaact gagaatgaaa
    13021 aagttgtagc tattggagag attggattag attattatta tgattattct ccaagagaag
    13081 aacaaaaaga gtggtttaag aagcaaattg aattagctaa tgagctaaaa cttccaataa
    13141 taatacatga tagagatgca catggagata cttttgagat aataaagaat actaaaaatc
    13201 ctgaaatagg atgtgtactt cattgttata gtgggaatgt agagttagct agagaatatg
    13261 ttaagatggg ttgttatata tcaattccag gtacggttac ttttaagaat aataaaaaaa
    13321 ctagagaagt agtaagagaa atacctctag aaagattatt catagagact gattcaccat
    13381 atatgtcacc agagccacat agaggaaaga gaaacaatcc ttcccaagta tcttttgttg
    13441 ctgataagat agcccaggaa aaagggatat cttatgaaga agtatgtaga gttacaaaag
    13501 aaaatgcaaa gaaatttttt aatataaaat agacaaatta aatcttataa tgatatatat
    13561 aataagtcat tataagtata ggatgagagt gcctcaaaat gagtttttta gtttatgagg
    13621 cactcttttt tgtatgaaat caaatatatt ttcatcattt caacaatgaa aaagaagttt
    13681 atcatatttt gagatagttt gtttttattt atttaattca tattttaaaa tatattttct
    13741 taacattgtt aaaaaagaat ttacaagata ttcattagaa ttaactcaac tctaatattc
    13801 acttaactta agttgtttat tatattatta aagagaagta aacgaaaggt agattaagga
    13861 ggttgaattt tgtatacagt tgatagtact aagatattga taaatatgaa cttagagaaa
    13921 ctagaaaaga tatactcaat gataaaagat acatcaagta tttctataat taaagaacct
    13981 aacttggcta cagtaatggt aagagctaat gaaagtgtaa aaaacacaac atttaattta
    14041 ggagagatat tagttactga atgtagtgta aaggtagatg aaagcttggg ttacggaata
    14101 gtttcagaaa acaataataa gaaagccatt tacttagcag ttatagatgc agttttacat
    14161 agcaataaca gtaaatttga tgagttaaag aattacataa ataaaagtgt atatgaagag
    14221 aacttaaggt atgaacaaga gattattgat gaatttagct tgattaataa aacgaaagtt
    14281 caattcaatg ctatggatta aataggagga gaatcatggt aaattataca caagaaatat
    14341 atagaaagat gctagatagt gcttcatttc ctgggaaatt aaataatata gataatataa
    14401 atatagaaaa taatacaaaa ctattgaatg gagcaattgg tatagcaatc actttattag
    14461 accaagaagt taccttctat atagaaaatt atactcaaaa agatataaaa aatataagag
    14521 cgcttacaat gtcaaatcaa atagattata gaaaatcaga ttatatattt ttagatatta
    14581 attcagaagt ggatatatta cagattaaaa taggttcttt tgaatatcct gatgaaagtg
    14641 caactatagt acatcaagta aatgatttat caacgtactc tcaaactaag tatataaagc
    14701 ttgatttgag tggtcctggt ataaaatcta aaaatagtat atatataaat ggaattagag
    14761 aagaatttat agaaagatta tctaccataa ataaagatta tccaatagga atagatttga
    14821 ttttagtaga taaaaaagga gaaattgcat ttataccaag gtcatcaaaa ttaagttggg
    14881 aggtattgta gaaatggctt atgtagcagt taaaggtgga gaagaagcca tattacaggc
    14941 taaaaatata cttgagtttt atagggtaaa aggagatagc ttgcctatag atgtaaagca
    15001 gattgaggaa caaatgtctt atgctgtaga caaagtaatg tctgaaggaa gtttatacaa
    15061 caaggaacta tcagcattgg ctataaaaca agctcaaggt gatatattag aagcctcttt
    15121 ctatgtaaga gctttaaggt caacaatgcc tagaattaca tattcagaac ccataaatac
    15181 agaagaaatg agaataaaaa gaagaatatc agctactttt aaagatatac caggaggtca
    15241 gtatcttggg gcaactagag actatgaaaa tagaatatta aatgtagaat tattagaaga
    15301 agatgaaaaa aagaatagac attttagaga tagttttata aatgaattag aaacagtaga
    15361 tgaaatccct gaatttacaa aaatatctaa tatatttaga aaagaaaatt tattacaaga
    15421 agaagtttgt gaaaacaaat tggaagaagt tattgatgat attacaagaa atagtttaag
    15481 atttccagtt tctaggtcag caaagttaca ggcattaact agaggtgaaa gtggagtagt
    15541 gtcaggtttt gcttatgcta ttgtaagagg ttttggtgat gaacatccat atataagtga
    15601 acttagaact ggtaatgtat ttataaaagt aaaacatccc atctatgaag atgaagaaat
    15661 aataataggg gatatgctgg ttactgaatg tgaaatagtt tcaaaagcat cacaaaataa
    15721 gactgaaaat tctaaaacta attcggaaga taaaaataag atgtctttag gatatggtct
    15781 ttgtatgggg aataatgaga ataaggctat atctatggcc atactagaca aatctttgga
    15841 gccaaatagt gaaaaactag cacaagatga agaatttgta ttgcttcata tagatggaat
    15901 agattcatta ggatttatat ctcactttaa gcttcctcac tatgtagatt ttaaagcgga
    15961 tgtagaaaga ataaaatagt ttgtggaggt aagtttaggt gatatataac tatggttttt
    16021 tagacgaaaa cacaaaaaaa gaaataagaa gaaagatatt aaaagcaata tcaatacctg
    16081 gttatcaagt tccctttggt tcaagagagt tgcctatagc taagggatgg ggtactggag
    16141 gtttacaatt aacactatca ttaattggta aaaatgatgt agtaaaagta attgaccaag
    16201 gaagtgatga ttctacaaat gcttgtaata taaggagctt tattgcaagt gtaagtaatg
    16261 tagaaaccac aaaagataca ttagaagcta cattgattca aacgagacat agaataccag
    16321 aagaaaaact taaatctaat caaatactaa tatttcaagt accaatacca gaaaccctta
    16381 gaatagtaga acctagtgaa gtagaaacaa gaagaatgca ttcagaagaa gattatagta
    16441 ggatgtgggt atatctttat gaagatatag ttagatttaa tgatatatct atagcagttg
    16501 aatatccatg taaagtaaat gatagatatt taatgaatcc atcacctata cctagatttg
    16561 atataaaaaa attaaatatg tcagacaatt tattcttgtt tggtgcaggt agagaaaaaa
    16621 gaatttatgc tattcctcca tatacaaagg tagacccttt agagtttgag gattataagt
    16681 ttgaagagga aaagtttgaa ggaaaatact gtagtttatg taacagtaca aatacatttt
    16741 tagatgaagt atatgacagt gacactaatg aaaagtacta tagttgctca gatacaagtt
    16801 attgtgaaaa agttagatta aaaaataaca gtatagatgt gacaatagga ggtacttgga
    16861 atgagtaaaa taaatgactc taaaagatta gaaaatatag acaagcaagt attaatagga
    16921 agaaatatca atcttattta tggagatggg tgtgaaaagt gttttgaaag cacaggatat
    16981 gaaagcaata atatatgtaa gtattgtggt tctatagttg catgtaataa tgtaaattta
    17041 gaactttatg aaggagaagt ccttggaatt gtaggtgaat ctggttcagg aaaaagcact
    17101 ttattaaaaa tactgttttt ccaagaacat gcaaatagtg gagaagtata tatatcccat
    17161 tacaataaag acacaaatat actagagctt tcagaccaaa aaaagagata cataaagaat
    17221 cattttatgg gtattgtgta tcaaaatccc caccttggtt taaatttaga ctttagtagt
    17281 ggtggtaata tagcagaaaa attattgatg gcaaatttat ataatgttga aaaaataaga
    17341 gatagaggca aagaactttt aataaaaact aatatacctg tagaaagaat agaccataaa
    17401 ccaaaatact ttagtggggg aatgcaacaa cgagttcaaa tagcaaaagc tctttcaaat
    17461 aaccctccaa tattgctatt agatgaggtt acaacaggtt tagatgtttc tgtgcaagcg
    17521 gaagtattgg atttaataag agaaatccaa agagaactta agatttctat gatagttgta
    17581 tcccatgact ttgatgttat aaagatgctt gctgatagga caattgtaat gaaaaatgga
    17641 agtgtaattg agtctggtct tacagaccaa ataatggagg acccacaaca cccatatact
    17701 cagcaactta taaattcatt gctataaata tcgtttgtaa ataagcaaaa tgggggtttt
    17761 taataaaatg aaagaaacta tacttgaagt aaataatttt agtaaagact ttgagttaca
    17821 tgcattgaac aagactataa cggcatgtag taatataagt tttgaagttt caaaaggtga
    17881 gtttttagga ataataggta aatctggagc aggtaagtct accatattaa aaagtatcta
    17941 taaaacatat ataccaacaa ctggagaaat aatttttaat tcagaaatat atggtaatgt
    18001 agacctggct aaaattggag accgagaaat gataaatctt agaaagaaag aaataggata
    18061 tgtatcacag tttttaaaga cacttccacg aataactgca attgaactgg tagtacattc
    18121 tttaattgag agtgggtttg aaaaagattg ttcgtatgat atggcaaaag atatacttaa
    18181 ccaatttgaa ataaaggaaa atttatggga tgcgtatcca aataattttt ctggtggaga
    18241 aaaattaaga ttaaatttag ctcaagctat ggttaaaaag cctagacttt tgttacttga
    18301 tgaaccaact gcatcacttg ataatcaatc taaaatatat gtaaaagaaa aaatgttgga
    18361 gcttaaaagt caaggaacta caatgatagg aatttttcat gatatagagt ttatggaaac
    18421 tgttatagat aagaccttta ctatggcaaa agggactata agtgaaagtg gggtagccta
    18481 gatgataaaa ctaattcata atgcaaatat tgtattagaa gatatgattt tagaaaatgg
    18541 tcatttaatc atagagggag atacaataaa aaaagtatca aatgataaga ttgattacgc
    18601 atttgacttt aatgagatag atgaaataat agatgcaaaa aacttatatg taatacctgg
    18661 tattatagat attcacagtg atgccattga aaaggaaata gagccaagac caagtacatt
    18721 attaccattt aatatggcat tttatgaact agataaaaaa ctacctgcaa atggaataac
    18781 tacagtttat cattctatat ctttagggga tggagtagga gttagaagta ttgataactc
    18841 tttaaagatg attaaaaata ttgattcata taaaaatata gatagtaaga gcataaatca
    18901 taaagttcat ttaagatatg aagtgttgta ttatgaaggt ttagaaaagg ttttagaact
    18961 attagatgaa aataaaatag actacttatc aataatggac cattctccag gacaaggtca
    19021 gtatacaaat ccaacatttt ataaagagta tgctactaaa gtgtggggag ttacagaaaa
    19081 ttatgtagat acatggttag atgatttagt aaatctgcat gataatttag attggaataa
    19141 aatagctaat attataggta tagccaaaac taaaaatata aatgttgcat cacatgatga
    19201 tgatacatta gaaaaaatgg atttcataaa aaatataggc ataaatgtat ctgaatttcc
    19261 tataactctt gaagttgcaa aacattcaaa gaaattgggt gttctaactt gtttaggtgc
    19321 accgaatatt gtaagaggta aatctcataa taacaattta aaagcaatgg atgctatatt
    19381 ggaagattgt tgtgacattg tgtgttcgga ttatcttcca tcagctatga taaagtctat
    19441 gtgtatagtt gcagaaagaa taaataattt aaataaagca gtcagtttat ttacctcaaa
    19501 tcctgctaaa gcagttggta tatatgatga aagaggtagt ataaaagaaa ataaaaaagc
    19561 agatttagtt ttagtagata ttgatagtga atatccaaag gttgtaaata ctattgtaaa
    19621 tggaaaaaca gtttataaaa gggaggtata aagattgcta acagagttac attgtcatac
    19681 aagtatatca gataataatt ttactactga agaaataata aaaaaagcag cagaaaagaa
    19741 tataaatatc ttagcaataa caaatcatga cacattatct ggattggatg aagctcttgt
    19801 gcttggagag cattatggaa taacaataat accaggtata gaaatttcag cctatgatta
    19861 taaaaataga aaaagagttc atattcttgg atataacata gatttaaaca gtcaagaaat
    19921 aaaaaatttg tgcaatccaa tggtatgtga cagacataaa gcatctgtta agatggtaaa
    19981 taaaattata gatttaggat acaaaatatc tctggaagat gtaaagaaat attcatcaaa
    20041 gactggaata tttaaacaac atataatgaa agctcttata gataaaggat atacagataa
    20101 tatatattca aatctatata aagaattgtt tcatagagga aacggcaagg tatatgtatc
    20161 tttaaaatat gtggattaca gagatgcaat aaaagctata aaaaattctg gtggaatttg
    20221 tgtgttagct cacccgggtc aaatggataa ttttagcgct attgaagaga tggtaaaagt
    20281 aggattagat ggaatagaag tatatcatcc atctcacaac aaagaactag aaaaagagtc
    20341 gctttcttat gctaagaagt ataatctagt ggtcacagga ggttcagatt accacggatt
    20401 ttattctcaa cataattatg aacttggaag caagagctta aataaagaag atttatttaa
    20461 gtttaggtta gctttaggag gggtattata aatgtctttt ttacaagtaa aaaatgtagg
    20521 taaaagttat ggacaagtta aggtgttaaa agacatatct attgatattg aaaaaggaga
    20581 gtttatttgt ttactaggtc caagtggttg tggaaagagt acactactta gaataattgc
    20641 agggcttgag gataaacatg gtggaaaaat aataataaat gataaggata tgactaattc
    20701 accacctgaa agtagaaatt ttggtatagt ttttcaatca tatgcattat ttccaaatat
    20761 gaatgtttac aaaaacatag cctttggact agaaaataaa aacataagta aatctaatat
    20821 tgataaaaaa gttaaagaag ttctagaagt tgtagaatta agtggatatg agaaaaaata
    20881 tccatcacaa ttatcaggtg gacaacaaca aagggttgca ttagctcgtg cgattgcttt
    20941 agagccagac tttttacttc ttgatgaacc tttatctgca ttagatgcaa aagtaagact
    21001 taaacttagg gagcagatta gaagtttaca tagaaaacta ggcattacaa ctataatggt
    21061 aacgcatgac caagaagaag ctctttgttt agctgataag atggttgtta tgaatagagg
    21121 agaaataata caagttggaa cgccaaagga ggtatataaa aatccagaaa caccttttgt
    21181 agctgatttt ataggaacaa taaattttat agatgatggt attaataaga tagctattag
    21241 accagaagat ataaaagtag agtctaatag agattctaaa gataaggata taaaagtggg
    21301 agaaatttta gatatcgaat ttagaggatt taactataga attacagttg agtatcgttc
    21361 taagcaaatg aaattagatg tagtttccaa agttgcagaa caaatgaaac tgtgcatagg
    21421 ttctaaaata aactttaaga tacctaaaga aggaattgta caatataaat cagagggatg
    21481 tgcttaatag ctaggggtga ataaatgata acatcaaaaa ataaaagaag aataagtgat
    21541 agcacaatac aaaaatattt cttgattttt tcggtaattg tactaatagt atttatatta
    21601 tttccattag tatcattatt aaaaaatgca tttatggata caagtggaaa ttttatagga
    21661 atttcaaact ttagtaaata tgttgaaaat ccaagtctta tagcatcatt taaaaattca
    21721 atttttgtat ctacagtctc atctttaata tcactatgtt tagcatttat atattcatac
    21781 gcaataacaa gaacaaacat aaagtttaaa gattttttta aaactatggg aatgtttcca
    21841 ctttttgcac caactatgtt atatggtatt tcacttatat acctttttgg aaataaggga
    21901 atctttacaa ccatgggtct ggaagtacca ctatatggtc cattaggaat aattatttct
    21961 cagattctat ttacatttcc acaagctttt ttaatattat ctgtagcact ttcgatggct
    22021 gattatagac tttatgaagc agcagatagc ttgggtgcta gtgaatttaa gaaattttgc
    22081 aaaataacaa ttcctggtat aaagtactca ttacttagtt cattctttgt atcctttata
    22141 cttgcattta cagattttgg agcagctaag gtagtcggag gaaactacaa tgtactggct
    22201 actgacatat ataaacaagt agttggacaa tttaacatac caatgggagc tactgtttct
    22261 atggtaatgt taattcctgt actaatagca tttacattag ataagatggc aagtaagaaa
    22321 caaggtatga ccataactgc aaagtctatg ccatataaga ttaaggaaaa taagtttaga
    22381 gatagagtat ttttaatagt atgtagtttt ataacattgc taatttttat tttaatattc
    22441 gtttcagtta taggttcagt tataaagtta tggccatata atttaagctt tacaatggaa
    22501 cactatacat ttaaaggtat tttgggaagt ggaataaaaa catacttaag cagtttgaaa
    22561 atatcaactt taacagcaat atttggaact atatttgtat tttttagtgc ttatatgata
    22621 gaaaaggtgg ataagtttcc taaattaaga caaacagctt accttttatc aatgatacca
    22681 atggccttac ctggcttagt tttaggtata tcatatataa ctttttttaa ctcagcaagt
    22741 aatccattaa attttttata tggaagtaca gcaatattag ttatagtaaa tgtagttcat
    22801 ttttattcag tttgttttat aacctcaaat tcatctttaa aaatgttgga taaagagtat
    22861 gaattagttg caaaatccat aaatattcca ttctataaag tattttttaa tataacagtt
    22921 ccaatgtcta taacatcaat acttgaaata gtagtatatt attttgtaaa ttcaatggtt
    22981 actgtttcag cattaatatt tttatataca cctcaaactc agactgcatc aatatcaata
    23041 ctaaagttag atgagatagg atatatagga ccatcagctg ctatggcagt attagtgctt
    23101 ttaacaaata taatagtaag acttttgtac gaatttgtaa caaagaaatt aaaaaataac
    23161 acacaaaaat ggcagcaaaa agatgttgca taggggaggt aaaaatgaaa tttaagaagc
    23221 tagtttcttt aggagtatta tcaacacttt tattaagtgt aataacagga tgtagtactg
    23281 attcatcagg ttcagattca aagtctgata gtaagacaaa aggggattta acagtttaca
    23341 cagcaattga ggaagatagt atagagccat atttagctac atttaaagag aaatatccag
    23401 atattaaatt aaatattgtt agggcatcaa caggagatat aacagcaaga ttattagcag
    23461 aaaaggataa tcctcaagca gatgttgtat ggggagttgc agccacaagc ttattggttg
    23521 cagatgacca ggggatgtta gaaccatatg ctccaaaagg ttctgaagaa atagagtctg
    23581 catttaaaga tgataaagaa gttccttctt gggttgggat agatgcttgg atgacaggta
    23641 taacagtaaa cactaaagaa ttaagtgata agaacatccc agttcctagt tcttacgaag
    23701 atttaataaa gccagaatat aaaggtctta tatctatgcc taatccatct tcttctggta
    23761 cagggtactt aacagtatca gcccttatac aaattatggg tgaagaaaaa gcatggcagt
    23821 atatggataa attgcatgag aatattggtg tatacactca atctggttca gctccagcta
    23881 catcagcagc ttcaggagaa tatcctattg gtatatcttt tggatataga ggtataaagt
    23941 taaaagagga aggctatcca gtagaagtag tatttccaaa agaaggttct ggatgggata
    24001 tagaagcaaa tgctcttgtg aaaaaagata atataaaaga agcatcaaaa gtatttttag
    24061 attgggctat aagtaaagat gcaatgaacg aatactctaa gaactatgca gttactacta
    24121 tttctacagg aaatccaata ccagaaggat tccctaaaaa gccattagag caaatgatag
    24181 ataatgattt aaaatcagca gcaaaaaata gagaagatat actaaataaa tggatatcaa
    24241 aatatgatgg taagacagaa aaagagagtt aagtaaaact atacaaatgg agtaaaaaga
    24301 aagaattttt ttaagtagat tttattataa taatataagt tgagaataat ataggtcaag
    24361 aaaagtgaat attctataaa aatgtataga gtaagtatac cattcagttt gaatggtata
    24421 ttttttattt ttagagatta atttagaaat agatatacta cagtttaaaa tag
//
LOCUS       DACXMZ010000036        24391 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.036, whole genome shotgun sequence.
ACCESSION   DACXMZ010000036 DACXMZ010000000
VERSION     DACXMZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.036"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            70..444
                     /locus_tag="JD016_05725"
     CDS             70..444
                     /locus_tag="JD016_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAU4999132.1"
                     /translation="MIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTTL
                     TLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALHD
                     DENIGKDKLDFLESYFDNLKDE"
     gene            437..1585
                     /locus_tag="JD016_05730"
     CDS             437..1585
                     /locus_tag="JD016_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HAU4999133.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENAVRNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNTNEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1602..2204
                     /locus_tag="JD016_05735"
     CDS             1602..2204
                     /locus_tag="JD016_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAU4999134.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2297..3319)
                     /locus_tag="JD016_05740"
     CDS             complement(2297..3319)
                     /locus_tag="JD016_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HAU4999135.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKIRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3648..3830)
                     /locus_tag="JD016_05745"
     CDS             complement(3648..3830)
                     /locus_tag="JD016_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999136.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4143..4562
                     /locus_tag="JD016_05750"
     CDS             4143..4562
                     /locus_tag="JD016_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HAU4999137.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            4693..6534
                     /locus_tag="JD016_05755"
     CDS             4693..6534
                     /locus_tag="JD016_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAU4999138.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            6549..8447
                     /locus_tag="JD016_05760"
     CDS             6549..8447
                     /locus_tag="JD016_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAU4999139.1"
                     /translation="MVIYLKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKD
                     LLNTKLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIY
                     IYLTLSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVI
                     ALVEEVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSI
                     EAFCKCIISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKED
                     MNKEAEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSK
                     NIYKSFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKIS
                     IFYGLIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESL
                     RLLVDDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYND
                     NYGHLAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELY
                     IKRVFKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKA
                     GRNQYIII"
     gene            8464..10455
                     /locus_tag="JD016_05765"
     CDS             8464..10455
                     /locus_tag="JD016_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAU4999140.1"
                     /translation="MRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLMENQ
                     GRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVVLIL
                     GDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACSFAR
                     QYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAIDSNG
                     SIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINNLIV
                     NTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKDKLT
                     KEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAENLL
                     ESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEERQIM
                     RIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRREDIIP
                     LFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYVVTG
                     KIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKEVNK
                     YVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            10717..11976
                     /locus_tag="JD016_05770"
     CDS             10717..11976
                     /locus_tag="JD016_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAU4999141.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKGKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            12037..13824
                     /gene="iorA"
                     /locus_tag="JD016_05775"
     CDS             12037..13824
                     /gene="iorA"
                     /locus_tag="JD016_05775"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HAU4999142.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            13826..14401
                     /locus_tag="JD016_05780"
     CDS             13826..14401
                     /locus_tag="JD016_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HAU4999143.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            14454..15524
                     /gene="buk"
                     /locus_tag="JD016_05785"
     CDS             14454..15524
                     /gene="buk"
                     /locus_tag="JD016_05785"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HAU4999144.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            15739..16443
                     /locus_tag="JD016_05790"
     CDS             15739..16443
                     /locus_tag="JD016_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999145.1"
                     /translation="MMGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGL
                     SIKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHAS
                     NTYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEIL
                     ALVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKS
                     NEHKCKSSIVIKNEQN"
     gene            16503..17009
                     /locus_tag="JD016_05795"
     CDS             16503..17009
                     /locus_tag="JD016_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAU4999146.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            17184..18206
                     /locus_tag="JD016_05800"
     CDS             17184..18206
                     /locus_tag="JD016_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAU4999147.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(18353..18661)
                     /locus_tag="JD016_05805"
     CDS             complement(18353..18661)
                     /locus_tag="JD016_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HAU4999148.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(18721..19149)
                     /locus_tag="JD016_05810"
     CDS             complement(18721..19149)
                     /locus_tag="JD016_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999149.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(19372..20829)
                     /locus_tag="JD016_05815"
     CDS             complement(19372..20829)
                     /locus_tag="JD016_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HAU4999150.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            21247..22161
                     /gene="nadA"
                     /locus_tag="JD016_05820"
     CDS             21247..22161
                     /gene="nadA"
                     /locus_tag="JD016_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAU4999151.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            22174..23475
                     /locus_tag="JD016_05825"
     CDS             22174..23475
                     /locus_tag="JD016_05825"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAU4999152.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            23480..24292
                     /gene="nadC"
                     /locus_tag="JD016_05830"
     CDS             23480..24292
                     /gene="nadC"
                     /locus_tag="JD016_05830"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAU4999153.1"
                     /translation="MIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQDGIIAGLGV
                     FKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYLQRMSGIAT
                     ITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGILLKDNHIG
                     AAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSLEMAKQAVD
                     IINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLRNI"
BASE COUNT         9626 a         2602 c         4174 g         7989 t
ORIGIN      
        1 aagtgtttga ctaaaaaatt cacatgttgt agaattaagg taacaggagt tttgagggag
       61 gtgtaaataa tgataagaaa aataccacaa gcagaactaa aagtaatgaa attcatttgg
      121 aaagtagatg ttacagtgac atcaaaagat gttattgaag ctatggaaca aaaatatggc
      181 tggaaacaaa ccaccacact gacactttta tctaggctgg taaaaagagg attcttagat
      241 gctcaaaaaa tagacagata tacacattac acaataagag taaaacacaa agattattta
      301 agttttgaga caaaggattt cctaagtaat atacatgatg actctttaag aagcttaatt
      361 tcagcattac atgatgatga aaatataggt aaagataagt tagattttct agaaagttat
      421 tttgataact taaaggatga ataattatat gctatatgtt tttgaaaatc ttataaagac
      481 aactattgta tgtagtcttg gtatttgtct attattattt ttgaaaagat atttatttaa
      541 gaagtttagt aagagattca attattatat ttggttgata atagtcttta gaatgttact
      601 ttttctattt aattatacaa tagtatatga agtaaaagaa cccaaagaaa atgcagtaag
      661 aaataatatt acacaaattg atatttctac agataataat cttatgctgt atgtagctta
      721 tttatggtta tttgtaacta tagtcattgc agtttataca tttataaagt atacaagatt
      781 taaaaatcta gttgttgatg tatcttatga tattgaagat aatgatataa actgtatcta
      841 caaaaacttg ttaaaagaat taaatataaa aaagaaaatt gaattaagag gttctgatga
      901 attgataagt ccagcaggca tgggattatt taaatcttac atattcttac cagactaccc
      961 ttatagtaaa gatgaactaa cttggatttt gaaacatgaa ttaatgcatt ttaaaaataa
     1021 agatatttta ataaagtttt tagttttgtc tgttagaata atatattggt ttaatccact
     1081 tgtatatgta atgagcaata aagtaaatct agattgtgaa ttatgttgtg atgaaagtgt
     1141 tttggtagaa tgttctttaa aagataaaaa agaatatgca ctagcactta taaagtcaat
     1201 taagtttagt aaaaattata atagtaagat tttgacaacc gagtttgata agacaaattt
     1261 ggaaaagaga cttgagagta ttgttaaaaa gaaaggcaaa agtggaatac taattgctgt
     1321 tttagtattt atgacttcta taacatcttt tgttgaagtt gatgctcaag ttagaattga
     1381 tgagggtaat aatggaatga ataacaatac taatgaattt ttattgaata agacgactga
     1441 taaaattata aatatttatt ataaagatgc accaaatgaa attaggaaat tttatgaaga
     1501 taaatgcgaa ttagaaggca aaatacctag agactatgac ataatagaaa tggatatgaa
     1561 agattataat caattaataa aataattata aggaggattt tatgttaaca aaattaagaa
     1621 aaatagtagc tattatgtgt tgcttattgc ttacttttac aagtgtggca tttgctgaca
     1681 caagtaaaga gaaaggagga agtaaagaga ctgttccaga gaaaaaccta gattctttta
     1741 ctctttctgg gtatatatat acatatgaaa atgcgccaca agaaataaga gatgaacata
     1801 aacaaaattg tgaagagata aatatagacc ctaagccaga tgatgagata tttgttccag
     1861 aatcagctaa tctaatgaat gaggacaata gtaaaggtgt ttatgcaagt tatactgttt
     1921 cttataatat aggtgctaaa actataacaa ttatgagtaa tgagtatttg actataagta
     1981 caacaaaagt tatacgaaag ggtaatagcg gaaaagaagt aaaagctgca caaatcatgt
     2041 taactttact tggttataat gtaggtatag atagttcttt tggaagtaag acatataatg
     2101 cagttgtaag tttccaaaaa aaatacggtt tgtctgcaga tggaataatt ggtccagcta
     2161 cttgggataa attgggtaga ctaactgacc ctactctttc ataggattaa aatctaaaaa
     2221 tatattaaat agtgtctcaa tatgattttt caatttaaat gagacactat ttaaatataa
     2281 atgttataaa actattttac aataaattcc atataactct accatctata tccttaacat
     2341 tttgacaacc agtcaaaatc atagtctcac ataactcatt cctaatcttc tctataaaaa
     2401 actccacacc atcaagacca ccgccaaaag aagcagtaac aaaaggtctt cccatcaaaa
     2461 cagcatcagc acccaatcca agcatcttaa ctacatcaac accagtccta actccaccat
     2521 caacaagtat tgttatcttt ccctttacag acttagctat atctggaagt acctcacaag
     2581 taccaggagt ataatcaagt actcttccac catgatttga aacaataatg gcactagcac
     2641 cagactctac agccatcaaa gcatcatcta cagtcataat tccttttata ataaaaggta
     2701 atctagtgga ttcaaccaat tctctaatct catcaattgt tttaggagaa aaaggatttt
     2761 cttgaaattg attattaata agtccacaag catcaaggtc tacaccaact gcaaaagcac
     2821 ctgcttcttc agacaatcta attttatcta ttatctttga attattccaa ggctttataa
     2881 atacaattcc atttccccta ttatccttca atacatctaa attatccagt aaaaaagtat
     2941 ctacattagt atcgccaacc atagcataaa ttccagaatt agaacaacct ctaacaacag
     3001 gctctatgta ttctttttcg gaaactttac cacccatgtt caatatagta cctgatacag
     3061 gtgcagcgaa tataggagaa ctcatttttc taccaaataa ttctatggat gtatctggtt
     3121 ttgaaacatt atgtattact ctcatattta tctttatttt ttctagactt tttctatttt
     3181 caatgaaaga cgaaccactt cctttaccac ccataccagg aacatctcca gcacaagcta
     3241 atccattaca aattttacat actttacatg ttccattaaa gttctctcta gctgatttta
     3301 gtaagtcatt gtaattcata caaaatctct cctttttata atattaaaat tgagtattac
     3361 tatattgaaa atatcttaat tgtaaatata gggatgttat tatgatgagc taaactagat
     3421 aatacatatt agatattttt tattgttgta gatagttaaa ttatatcata ttagatagtc
     3481 aaattttgaa aaaaactaac ttatttaagt atacattttg aaaaaattaa aaaagctata
     3541 tatagatttt taaagaaatc tatatatagc ttttaagttt atgtcaatct tttttgttaa
     3601 tgaataaata atatagaata caatatacaa aaaagtttct tgaaatgcta agataatccc
     3661 catttacaaa gcaacatcat tatactgtgt aaatttgaat caacaatgtt ttcaccatat
     3721 ttcatcataa aatcctctaa ttgtgtgtca ataaatgaat aatatccact tatactatca
     3781 atgtatgcag tcattaagtg tatacattca tcacttttat ctttatacat atatatctcc
     3841 ccctatatac aatatataaa tttagatacc aaatcgtatc atattaaata gttaagtgtg
     3901 taacatctat gcagttatat atttatatca ttatattaat aactaaagtg agttttgaca
     3961 atagattttg ggaaactgga tgttcaaagt gcaaacttgg attttaaaat cattaattaa
     4021 gatagcgcat aggaatatat gtatacataa cttaattata aagattataa acaattactt
     4081 ttattaactt tatttaaatg gtagaataac ttggagatta ataattataa ggaggatata
     4141 atatgagttt atataagttt atatatgatg ataaggaata tttattaaaa gaggagaact
     4201 gtagtgcttt aataaatgat gaagaaaatc cagttcaagg agtaagtatt tcaaaaataa
     4261 ttgatatact taatgaagca gaagaagttg attttgatgt agaatattat caagaagcat
     4321 gtccattatg tttagaagga gtaaaagaaa agaaaaaatt ctttcctttt ttagaatatc
     4381 acttttatat tttcagtaaa gatggagaat atgtaataag caatatatct gttgattata
     4441 aaggactatc ttttaataaa ctatcaaggg caaataaagt tgataatagt tatattgtaa
     4501 gtgtgataat ttgcgaaaat tgtcaggatt atatagttca gatagaaaat tgtatagttt
     4561 agtgtaaagt atgtcttaat ttaagagaaa tttctaaatt gagacatcaa cttttttcaa
     4621 aaatagtatg gaaaattact aaatttatga gataatgtaa gtcggaggtg agttacaata
     4681 tatagtaaat atttggaaga gatagatgat acaattttag attcaatgtt taaactaacc
     4741 ttgaatgaaa gattattgac taataacgat tttattgaaa ataaaattga tgtagtaaag
     4801 aatttaattg attgctataa aactgaacaa aatagtttaa tgttattaaa atcaaatttt
     4861 ttgctagctt tattatatgg tattcaaggg tttagtgaaa aaatgaaaga gtatattttg
     4921 atttcctgta aatatattga taaatgctta cccaaagatt ataaattttt agcacgattt
     4981 tattcacaaa tagctatttt aagtttaaaa aatgaagatg taaataaggc aaatttatat
     5041 atggataaat ttaatcttat ttgtgatgaa aataattttc taattgaaga aattattttt
     5101 aaatcacaac ttatttacat taaggcaagt aagtgtatag aatcttctgt aataattaaa
     5161 gaaatagaaa cgttgtatat aaagataaaa gaaattaacg attttaattg taaaaaaata
     5221 tattttttta ttattggtaa aatatatttt ttgtttttgg atgatgctct aattgctaag
     5281 gtaaattttt taaaagcaag aaaatatgca gatttactaa aggatatgca agtatcttca
     5341 ctatgtaata taaagatagg tgagtgtgaa tgttcgtttg aaaattatga atgttccatg
     5401 gaatatttca atgaagtaat aagcaataaa aagtatagaa atgtaaatat aattcaaaaa
     5461 tatagagcat caaacaacat aactaaaata ttaataaaga ctagaaatta tcctgtcgca
     5521 ataaataatt tagcaaaatc tgaagtatat ctagaaaaaa ttaaaaatca tagcttaaaa
     5581 gaagtagaga agtttgattt atttataagt ttagccatgt attatgcaaa aagtaatgaa
     5641 aaatcttttg aacaatcaat atgctattta gatagagcta aaaacatatt aaatatgata
     5701 aaaaatatag aagaatatat aatatatgat ttagaaataa tttatcacca ggcaaagata
     5761 tattatatct ttggatatta tgataatgcc ttaaacacaa ataaaaaact ccttgaaaaa
     5821 tctaaaaatg ctaaaaatta taattatgtg aagttagcat acaagagtat atatttgtgt
     5881 tttgaaaaga ttcaaaatta tgaaatgagt atgaaatact ttaaaatgta ttatgagtta
     5941 aaaatgatgt atataaaagc tcgaaataga aattatatag attccttaaa ttatagacat
     6001 gagtatatag aaaaagaaat aaataacatg agaaaagtaa aacttgattt agataaaaaa
     6061 aagtatgttg accatttgac aagtgcatat aatagaactt ttttgaacga ttttttagaa
     6121 aaacaagaag ttagtgaata ttgcacagca tttatgatag atgtagatta ctttaaagaa
     6181 tacaatgata cttatggaca ctacaatgga gatattgctc ttaaaaatat aactataata
     6241 ataaaaaaat atctaaaaaa agacatgctt atgataaggt atggtggaga agaatttctg
     6301 atattatctg tatgtaaaga ttataaaaaa agtaaaatat ttggaagaaa attatgtagg
     6361 attgtaaaga aatttttaaa caataatttg acagttagta ttggcattga tacatgtaaa
     6421 aatagtagta tagatataaa agaaataatt gaaaatgctg ataaagcttt atataaagca
     6481 aaggaaagtg gtagaaatag atgtttgcat tatcatgatt ttaagaattt ttaactgttg
     6541 aaataggatt ggtgatttac ttgaaaagga gtttaataga agatttaaag agaaatataa
     6601 acaagataga atacgatatg ttgactaaat tctatctaaa tttgacagat ttaaactaca
     6661 tcttggatta tcacaaagac cttttaaata caaaattaga agataatagt agagtctata
     6721 ctttatattt aattagtctt ataaactata taaaaaaaga tagagaagca tcatacaact
     6781 atttgataga gtcattaaag tatattaaaa aattgtcatt aaaagatgat tcagatataa
     6841 tatctaaaat ttatatttat cttactttat cagctatttc tataagaaaa tatgataagg
     6901 taaataattt ttatttctcc gcaaaaaaaa ttataagaca aaatcattta aatgagttgt
     6961 tggtattgtt aaatatgagt ttatgtgtgg aaataagtgc tttatatcag accaaaacgg
     7021 atgtaatagc tttagtagaa gaagtgtctt tttttaaaac tttaaaaaat aaagctcttg
     7081 taagtagggc aaattatata tttgggagag tttatctata tttatttaat aactttataa
     7141 aaagtatgga atacttaatt gaatgtctaa aattggctca agaaaataat ttatactcta
     7201 tagaggcttt ttgtaaatgt ataataagtt tgtgttattt agagagtgga aataatgtag
     7261 aagctataaa atacataaat aatgtattag atgttgcaca cagtaataac tcaatatcag
     7321 taactgaaag agtgtctata aaaatagacc ttatatgggc atatttaaaa gaagatatga
     7381 ataaagaggc agaagttatt ttattaaaga ctataaaatt aatgaaagtt gttgaaggtg
     7441 tatataagga ttatttatat tcatttatat ttttatattc agctgaaatt gagttaaaga
     7501 aagataattg tagttttgaa aaggtaaaca cctatttaat tttatcaaaa aatatatata
     7561 aaagttttgg agatgttttt atatataatt ctaaactaga ttacatatat aagttgtatg
     7621 gagatttata tataaaattt ggtaatatag atgaaggtat aagttgttat ataaaaggtt
     7681 taaatttagt taaaaagagt tcattgaatc ttagaaaaat atctatattt tatgggctaa
     7741 tagcaaatgg atatgagcta aaaaaagatt ttaaactggc aatagaatat tataaaagta
     7801 tggattggta ttctgatagg tgggaaaaaa ataattccta taaaacatct caagcaattc
     7861 acaaacaata tgaactcaga caaaaacaag agagtttaag gttactagtg gatgataata
     7921 agaaattaga aaatgatatt ttaaaagatg gacttactaa actatataat agaagatatc
     7981 tggaacagca gttggattta tttaatggaa ccagagacaa aaatatggtc gcaatactgg
     8041 tcgatataga ttgttttaaa agttataatg ataattatgg tcatctagca ggagataaag
     8101 ttatagttaa ggtaactgat attataagaa gtgtatttat agatattacc gaacacataa
     8161 tcagatatgg gggagaagaa attttaatat tagtagattc tatagaatca atcggtgttg
     8221 aagagattga taatatagaa ttatatatta aaagagtttt caaacttctt gaatttgagg
     8281 gtattgaaca tatatattct aaaataaaaa actatataac tataagtgct ggagtaagta
     8341 taaaaagatg tcgtagtaga aaagatgttg aaatattaat tgaagatgca gataagaacc
     8401 tatataaatc aaaaaaagca ggtagaaatc aatacattat aatttaaaat tgaggtgatt
     8461 ttgttgcgaa aaaaaatagg aattatagcc tctgatattg aattaaaaga aagaatagaa
     8521 gaattatata gagaagatgt agaaaatgga actataataa tcgacatact taacctagac
     8581 ttaatggaaa atcaaggtcg tatacttgtt gaaaaaggtg cccaagctat aattggtaga
     8641 ggtggaggtt atagtttagt aatagataca gtaaatgtac ctgttatacc aatgaatatg
     8701 aagtcaactg accttttaag agcaatagaa atagcaaaga aatattctaa aaaagtagta
     8761 ttgattcttg gagacaatga agtttctttt gactatgttg gctggagaaa tgtcataagt
     8821 actgaaataa ctgaagaatg gtttgaatct aagtatgaga taagaagtaa agttgtaaaa
     8881 tatatagacc aaaaagatga agttgtaatc gtaggaggag gtttggcatg ttcttttgca
     8941 aggcaatatg gaattgatag tgtatttgca actgctagtg atgaatctat aagagaagct
     9001 gtagaatatt gtaaaaaatt attagataca ttaggtgaag aaaagtttaa taatgaagtg
     9061 ttacgaaata tacttgatgg tataaaagat ggagtaattg ctatagatag taatggcagt
     9121 ataattttgt acaatgaaag tgcaaaaaac atgcttaaag tagagagaaa atgtgcactt
     9181 aataaataca tcttagatgt atttcctaaa atggagtgga tgttagattg tttacatgaa
     9241 aaagaggacg tagaagatag aaagataaga aatataaata atctaattgt taatactagg
     9301 acaacattga taaaagttga taatagtact tatggagttt taggaataat acaagatata
     9361 accaagctac aaaacttaga aagaaaaata agatttgact tgaatcaaaa aggtctctat
     9421 gctagatata cttttgatga ttttttattt aaagataaat taactaaaga atttattgaa
     9481 gaagctaaaa aaattggaaa aagtgattat acaactttac tttatggaga aagtgggtct
     9541 ggtaaagaaa ttatagctca tagtatacac aacataagta aaagaaaaga taggcctttt
     9601 gttgctataa attgtgctac tatagcagaa aatttacttg aaagtgaact ttttggctat
     9661 gaagaaggtg catttactgg tgctagaaag ggtggaaaaa gaggactatt tgaacttgct
     9721 catggaggga ctcttttttt agatgaaata aatagtctat cctttaatat tcaaacaaaa
     9781 ctattaagag taatagaaga acgtcaaata atgagaattg gttctgatta tataatacct
     9841 ctggatataa ggattatagc agcaacgaat gaatctttaa ccgaaaaaat agttatgggt
     9901 acatttagag cagacttatt ttatagattg agtagtttag agataaatat acctccttta
     9961 agagacagaa gagaagatat aatacctttg tttaacaatt ttgtaaatga agtattaaag
    10021 gatgatggtc tcaatggaat aaacagtata gatgaaaatt ttgttttgac taaagatgaa
    10081 atggataagc tatataatta tagctggcct ggaaatgtaa gagagttaaa gacaatagct
    10141 caaaaatatg ttgtgacagg taaaattaaa ttgagacaag atagagattt taaaactaaa
    10201 aaattacttc ctaactctga agtagacaaa tttaattctg aaacgacagc aagtgtagaa
    10261 gtccaagatg aatctataaa tataagtaag ataaatgatg gtaagataag tatagatata
    10321 aaagaagtaa ataagtatgt tgaagaaaaa ataataagta tgctttttgc tcagggactt
    10381 tctaaaaatg aagttgcaca agttcttgga ataagcagga catctttatg gaagaaatac
    10441 aataaaaata tttaaatata attacatata aattattttg aagtaatgtt aattttttaa
    10501 aacaagtgtt aagaaaaatt aacattactt ttttgcaaat ttttattttt ttagacaggc
    10561 atatatattt ataagaaaaa tagttaaaac acttaattta ataatgaaaa aaataataat
    10621 tctaaacaaa atcaatttta cataaagtgg gatttattgg catagtaatt gcttaatata
    10681 taatgtaaga gtaaatacaa ttttaggggt gatggtatga gtcaaagtat ggttggtaag
    10741 catgcaatgt ggccaaaaga aaatgatgtt atatttagca tatctggcag agctcaagca
    10801 gcagagaagg cttttggtat ggataatgta ataaatgcaa caataggcgc tctaatggat
    10861 gattcaggaa aattgataac aatgaaaact gtatatgaag aatataaagc attagataac
    10921 tgtgagatag gtgcatatgc agctcttgaa ggtcaaccag attacttaga agcagttaaa
    10981 aaagtatttt tcagagatta cttaccagaa ggacatataa gagtattagc ttcaccagga
    11041 ggaagtggtg caataaaatt agctgtatgg aactatacta atgaaggaga tgaggtatta
    11101 acatctgatt ggttctggag tccatatgta agtatagctg aagaggctaa tagaaaagta
    11161 gttaattacc aactttttga tgaaaataga aggtttaatt ttgagtcgtt taaagaaaaa
    11221 tttgtaaata tagcagaaaa acaaggtaga gtctttacaa ttataaacac accagcgcat
    11281 aatccaactg gatatagtgt tgcagatgat gagtgggata aaatattaga cttatcaaaa
    11341 gaagtcgcaa aagataaagg caaaaagata atatttttcg tagattcagc atatattgac
    11401 tttgcaggtg atgatgatgt atgtagaaag ttttttaaga aattttctaa tttgcctgaa
    11461 aacgttctcg tattggttgg attcagtatg tcaaaaggat ttacagctta tggtatgaga
    11521 atgggggcta taatttgtat atcttcaagt gaagatgtag ctgaagagtt ccattactca
    11581 tgtgttcatt catgtagagc aaattggtca aattgtaata gaagtgccat ggcagtatta
    11641 tctaacattg taaatgaccc taaaaaattc aaagaatatg aggatgaaaa agaaatatac
    11701 aaaaacatgc ttactagaag agcagatgta tttgtaaaag aagctgaaag agtaggatta
    11761 gaaattcttc catatatagc aggtttcttt gtaagtatac catgtgataa tccaaaagaa
    11821 gtatgtgaag aattgactaa acataattta tttgcagttc cattaaaaat gggcttaaga
    11881 tttgcagttt gtgcagtttc tgaagataaa tgtaagaaag caccaagtat tataaaagaa
    11941 gcacttgaaa gtttagaagt aaaaataaat aattaatttc attgaacaaa ttgggtggac
    12001 tatgaattta ataattgtag atggagggaa ttttatatga aacaattgat gacaggtaat
    12061 gaagcaattg cacgtggagc ctatgaggct ggtgttaaat atgcatctgc atatccaggg
    12121 actccaagta cagaaatatt agaaaatata gcaacatata aagatgcaat agttgcagag
    12181 tgggctccaa acgaaaaggt agcacttgag gctgctatag ggggttcaat tgcaggagca
    12241 agaacaatgg cttctatgaa acatgttggt ttgaatgttg cagctgatcc aatatttact
    12301 tatgcatata caggggtaaa tggtggtatg gtacttatta cagcagatga gccgggaatg
    12361 cattcatctc aaaatgagca agacaatagg atgtatgcaa aatttgcaaa gataccttta
    12421 tttgaaccat caactagtca agaagctaaa gatatgataa aagaagcttt tgaagttagt
    12481 gaaaaatacg atacaccagt tttatataga gttacaacaa gactttgtca ttcaaaagga
    12541 ttagtggagt gctatgatag agaagaagtt gaaattaaag agtatgttaa aaatgctaag
    12601 aaaatggtaa ctgttccagc aaatgctcaa ataagaaggg gcgttgttga agaaagaatg
    12661 gaaatcctta aaaaattctc aaatgaaact gatttaaact actatgaaat aaatgataca
    12721 aaaataggtg ttatagcctc tggtatgtgt tgtaattttg ccaaagaagt ttttggaaaa
    12781 aatgcatcat atatgaaact aggatttaca aatccattac cttatgaaaa aataaaagaa
    12841 tttgcagaaa aagttgataa aatatatgtt atagaagaaa atgacccatt tatagaagaa
    12901 caaataaaag catatggaat agattgtatt ggaaaagatg taataccacc atatggagaa
    12961 atgacaccag atgttttaag aaaagctata tttggaaaaa caaatgatac tatagaatac
    13021 aaatctgaat tagtaacacc aagaccacca agtttctgtg caggatgtcc acatagagga
    13081 ttcttctatg aacttggaaa gagaaaaaat ttaatagttg gtggagatat aggttgttat
    13141 actcttggat ttgctcctcc atataatgga atagactatg ttgtttgtat gggttctgct
    13201 tttggaactg cacatggagc tcaaaaagtt cttaatatga aggatgataa tgaaaaaaga
    13261 ttggtaggtg tacttggaga ctcaacattc ttccatacag gaataaatgg acttttagat
    13321 gttgtttaca atagaggtaa ttctatttcg gttatattag ataatagaat aactggaatg
    13381 acaggtcacc aagaaaaccc aggttcagga tatactttac aaggtgctaa aactaaagaa
    13441 gttgatatag aaggattagt taaagcttgt ggaatagaac atgtaagagt tataaaccct
    13501 aacaatttaa aagaagtaaa tgaagcacta gattgggctt tagctataga agatgaacca
    13561 tcagtaataa taacaagatg gccatgtgta cttaaaaaat tctctaaaga agatatagaa
    13621 gaatttaata atccatttaa gactaagtgt aaagttgacc atgataaatg tataggatgt
    13681 aaattatgtt tgaaaactgg atgtccagct ctatcatttg ataaagagaa taagttatca
    13741 aatatagata gaaatcagtg tgtaggatgt ggagtatgtg cacaagtttg tccaaaacaa
    13801 gcaatagtta aggaggaaaa ataatatgac taagagtata cttttggtag gtgtaggagg
    13861 tcaaggaact atacttgcta gtaaattatt aactatggga ttgatggaag ctggatatga
    13921 tgtaaagatg agtgaaatac atggaatgag tcaaagagga ggttcagttt cctcacaagt
    13981 tagatatgga gattgtgttc attctccagt tatagaaatt ggtggagcag atatattagt
    14041 atcttttgaa aaaatggaag ctttaagatg gttcaattat ttaaaaccag aaggaaaagc
    14101 tttagtaaat aatcatagga tagattctat gactgtttta atcggaggag ctgaatatca
    14161 agaaagtgat atagatgcag agttaagtag attaaatgca aaagttataa atgcagctga
    14221 taaagctgaa gaactaggaa attcaaaaat aatgaatgtt attttattag gatgtctagt
    14281 taaatctatg caattagagt ctattgattg ggaaaaaatt ataagtgaaa atgttaaacc
    14341 aaaatttgtt gaattaaaca taaaagcttt ccatgaaggt atggaaatgg ttagcaaata
    14401 gtttataata gatataaata gactatagaa attaaggtga aaggaagaaa attatgactt
    14461 acagaatatt agccataaat ccaggttcta cttctacaaa aatagcagta tatgatggag
    14521 aagaacaaat tcttgtgaag acgatagacc atccggctga agagattgca aaatataata
    14581 ctatacaaga ccagtttgaa atgcgtaagg aagcagtttt gaatattctt aaagaaaata
    14641 gtatagactt aaaatctctt agtgcaatag taggaagagg tggagtttta ccaccagtaa
    14701 aatcaggagc atatttagta aatgaagaaa tgattgatgt actaagacat agaccagtac
    14761 ttgaacacgc ttccaattta ggtgctgttg tggcacatgc aatatcagaa cctcttggaa
    14821 tcaactcata tatttatgat tctgttgcag tagatgagct tatagatgta gcgagaatat
    14881 ctggactttg tggaatggat agatcaagtg cagggcatgc attaaatact agagcaatgg
    14941 ctttaaaata tgctaaggat aaaggaaaag attataagag cttaaactta atagtagctc
    15001 atattggtgg aggagtaagt atttatcttc atgaaaaagg aagaatggtt gatatgctat
    15061 ctgatgatga aggaccattt tctccagaaa ggtcaggaag agtacctgct acaaaattag
    15121 tggctgcctg ttattcaggt caatattcag aaagagaaat gactaaaaag ataagaggta
    15181 aaggtggtat agtttcatac ctaaatactg tagatgctag agaagttgaa aaaatgatag
    15241 cagaaggaaa tgaagaagca aaaattattt atgaagcaat ggcttatcag ttagcaaaag
    15301 gtattggaga gttagcaact gtagtagatg gaaaggtaga tgctataatt ataacaggtg
    15361 gaattgcata ttctgaaatg tttacttcaa tggttaaaaa gaaagttgag tttatagcac
    15421 cagtagaaat tatggcagga gaaaatgagt tggaatcact tgcttttgga actttaagag
    15481 tactaaatgg agaagaagaa gctagaattt atagtgaaaa ttaataagaa aaactaaaaa
    15541 tgtttatata ctaaaaaagt ggtatatccg ttttttatac acgaaagctt agagtatagg
    15601 tgaaaatgta taaaatttgg attgccactt tttataatta aaattttatc ttaacctaat
    15661 aaataattct gtaaaatttc gtatttatat atatatttta ttggtattta ttgttattat
    15721 tattatgagg agtgattttt gatgggaaaa ataagtttaa aaagtatgat aagattattt
    15781 tcaggttttt ttatatttgc aatttcttct gtgcttatga ttaatgcaca tgtaggtctt
    15841 atgccttggg atgtacttca ccaaggttta tctataaagc taggcataac tataggtcag
    15901 gcttctataa tggtaggagt tgtaattgtt atattggatg cagtttttgg tgaaaatata
    15961 ggatggggta ctttactcaa catgacattt ataggaattt ttattgattt agtaatattt
    16021 tctggagtga tacctcatgc aagcaacact tatataggtg tgtttatggt tgttatagga
    16081 attattttgg cagctatagc aagttttcta tacttaggtg tttgtttagg aagtggaccg
    16141 agagatgggc ttatgattgc tcttcagaaa aaaactaata aatctgtacg tcttgttaga
    16201 acaattcttg aaatactagc attagttgtt gggtggttat taggaggttc tgtaggtatt
    16261 ggtacgcttg taagtgcttt aggtttagga tatgtattac aaatagtgtt tagaatattt
    16321 aaatttgaca caaaattgtt gaaacataga tttattatag atgatataag agaatggaag
    16381 gaaaagaaaa gcaatgaaca taaatgtaaa tcaagtattg taataaaaaa tgagcagaat
    16441 taattatgct aaaatatatt tgtagtttat ctaatttata gtaaagtcaa ggagattttg
    16501 aaatggaatt atgggattta tataatgctg atggaattaa aacaggaaat gttataaaaa
    16561 gaggtaattc tattgaagaa ggttattacc atttggcagt tgaagtgtgg attttaaata
    16621 gtaactctca aattttaatt cagaaaaggt ctaaaagtaa aaagacattg ccaaatatgt
    16681 ggggaatgac aactggatgt attgtttctg gggaagacag tttagaagga gcaattcgcg
    16741 aagcaaagga agaaataggg atagatataa ctaaagatga gatgaaagtt tttagaagta
    16801 tgattcatga agatactctt tgggatgtat acttagttaa aaaagaatat gatatatcca
    16861 aagctatttt acaggaggaa gaagtaagtg atataaagtg ggtaagcaca gatgaaataa
    16921 gacaattact aaaagagggt ttgttttttg aatatccaga gatatacgaa ttgctatatg
    16981 agatagataa taataaatta aatatatgat gtagagcaga tttcagtata agtaagatta
    17041 tttaaaatgt atcttctcta aattttaata ttgttttatg aaaattaata agttattttt
    17101 agtatttttc tacttgtttt ttgagattaa aataaaaagg gcagtcatta taaagtcata
    17161 aaaatttggg gaggatttaa actatggaag ataacaaagg attggcacgt gacagttcaa
    17221 aatataaaat tgtttctata tctgtcgtag taattattct aattacattt tggtatatgt
    17281 taaatatggt gcttttaaca tttattatga cttttatttt ctataacttg cttgtagcca
    17341 caagaaaacg aattaagaag ttttcttctt taaatattcc agattcattg ataattatag
    17401 tgttatatgc actatttgct atattgttag tattgattag ctatgcagtt gtaccaataa
    17461 ttatagttca acttacagag ttgagtaggg tttttagtga ttttgatgta aatcaatttg
    17521 ctcaatcttt aggaccaaaa ttgtatccta ttgtctcaaa gctggatttt aataaatata
    17581 tttcacaagc agggctactt atagcatcaa cagcaacaaa agttggaagt tttggagtaa
    17641 atatattatt ggcattttta ctgagtttac tccttttact tgaaaaaaat gaaattaaaa
    17701 attttggaga taaactttct gatagtaaga tttcttttat ttataattcg cttgtatttt
    17761 ttggaaagag ctttgttaaa aattttggtg aagtaatgaa agtacaagtt atgattgcct
    17821 ttataaattc tgttgtttct atgattttct taggtttcat gggattccct caaatatggg
    17881 ctttaggatt tatgattttt gtgttgggtc ttattccagt agcaggagtt atagtgtctt
    17941 tgattccatt aactgtaata gcatttaata caggaggaat aacaaaggtt tttggagttt
    18001 tacttatgat ttgtatagtg catgcagtag aaacatacat attaaaccct aaattgatgt
    18061 cgaatagaac taaacttcca gtttgttttg tttttattat tttacttgta ggagaacatt
    18121 atttaggtgt atggggatta ctaattggag ttccaatatt tatgttcctt atggatattc
    18181 tgggtgttaa atttacttct aggtaatata ttaaaataat aatttatttt tatatataaa
    18241 ttatgaattg ttttaaaaaa gaacctataa atagacatca ttaataaaga catctatttg
    18301 ataggttcaa ttttgctttg tataaatact ataaaactaa ataaaaagaa atttatttca
    18361 tttcaaaagt ttcacaactt gcatttcttt catttatatg aactttttca gctttgcaaa
    18421 gttcattttt attgtattta cattttacag cttcacaatg aatgttatct gtatcaactt
    18481 ggttagtttc attagaacga ctagttatac cagatgtata tttgtcttca aaagaagcac
    18541 aataagtatt ttctgtagta gttgcttgca ttccatctac ttttatacta ccagcataac
    18601 aatatccact agtattataa gcacaattag tagcagcaca atttaaatta ttatttttca
    18661 taaataacct cctaagtttt atttaaactt attatgtgaa gttatctaaa ttttatacac
    18721 ttatttacaa ttacatttat cacatttaac aactacatct ttggaagttt cccttcctaa
    18781 atttttatta acatgataca catcaacatt ttcagttgat ccacatatag aacatttaaa
    18841 attgttttct agtagggttc tttctttaaa taacttccaa tacatagaat tgatgtaatc
    18901 attataattt accttgaggc atgtctcaag aaagtttatg tatagattgt ctaaatctcc
    18961 tgtatttaat tctgtatatt ctggttcaaa atattcacaa catcctctaa aatcgggctt
    19021 tgtatctgaa ataacacatc taatatcata tctatagtct aggctaggcc ccttaaatga
    19081 aagatgtgtg caaaaagaac aagatgtttc tggtagattt ttttcaagtt catgtaattt
    19141 attattcata agtattcctc caaaaaattt attttaagta tataaacgaa tagattattt
    19201 taaaactttt atttatttat attagagatt taggataaat attccaataa ggagtaaata
    19261 cttaagtatt tattactgta ttaaataaaa aagaggcaaa tctaacttga gattgcctac
    19321 ttttttatca ttttttaata aaatttattc taagtattat tttatttaga tttagtcagc
    19381 caacttagta tctgctttgc tttttaactt tctaccagag cgtacagtta aaatagcata
    19441 taatacagta aatatggcag ctaaaacata tgatatattt atgtttaagt taaatccaat
    19501 ttgagcatta aatatataac tgaatataac aaaggaataa aacataccag gaattaagct
    19561 tataatataa ttcttttctt ttattataag gtaaactgta atcattgcaa atgcaaatat
    19621 ggcaatagtt tgattactcc aagagaagta tctccacaat atattaaatc cagaagcact
    19681 aagctttgca aatataagga tggctatgac aggaataaat atacagattg acaatataac
    19741 acgatgtttc ttttcctttt ggtcataatg aaagtaatct gcaagcatta atcttaaaga
    19801 tcttaaagct gtatctccag aagttattgg taaaacaatt acacctatta tagctatgat
    19861 accaccaata gaaccaagta aatctcttgc aaccaaacct attacatcag gagaacctac
    19921 taattcctta ggtattcctt tattgtaaat acccatagca gctgcagccc aaatcattgc
    19981 aattaaacct tctaaaatca tcatattata aaaagtagtt tttccttctt tttcatttga
    20041 aactgatctt gctataagag tagcttgagt tgagtggaat ccagaaacaa taccacaagc
    20101 aactgtaaca aagaatgtag gaataagagg aataccatca ggatgaatac ctttccaatt
    20161 tgcaagactt aaattagcta aatcataacc ttgtgtaaag attccaattc caacaccagc
    20221 agcagataaa agtagaatag ctccaaatat tggatatacc tttcctatga ttttatcaat
    20281 tggaaatagt gttgcacaca agtaatatag aaagataagt ccataaacta tccagacaac
    20341 tggattattt atagtcgatt tcatatttaa aatttgagta acaactaaat ccccaggtgt
    20401 ataaataaaa actgcaccaa ctaaaagcat taaaagacaa aggaatatat tatagacttg
    20461 aaatacttta ttacctaaaa atctactgat aagagaaggc atttgtgctc ctttttctct
    20521 tatactaatc attccactca tatagtcatg caaagctcct ccaaatacac agccaatagg
    20581 tattagtatg aatgctattg gaccaaacaa tataccttgg ataggtccta gaattggtcc
    20641 tgtaccagct atattaagca actcaattag actatttttc cattttttca ttgggacata
    20701 gtctactcca tcactttgag caagtgcagg tgtttttctg tcatctgggc caaatacttt
    20761 ttcacaataa gcaccgtata aagcacctcc tattagtaat atagccaagc cgattaagaa
    20821 tgttgtcata atatcacccc atttataaaa ttttttggtc tttgtataca tattatctaa
    20881 atgtgtagat ttatattgaa tttaattctg gacaacctac aaattctata aagtattaca
    20941 actaatataa tttcagaaat aaatacatat acataaccat gtacttagta tacaggtatt
    21001 tggtaatatt ttcaatgtta tagcaataaa gaatataaac aaaattctga taatatttta
    21061 caaaaaaatt aaaaaatata aaaattagtt taaatagatg gagaaaaaag gaaaatcaat
    21121 tgagaatata aatgtctata tcttaacaat attaagaggt ttaaaattct tgaagaaaga
    21181 atagtcaagt gatataatga gtttacaggt gtatatacac tagtacatat agaaagcggt
    21241 gcaattatgg ataaagattt aacatatcaa atcaaagagc taaagaaaga aaaaaatgca
    21301 ataatacttg cccattttta tcaaccacca gaaatacaag aattagcaga tgcagttggt
    21361 gactcatatt atttaagtga gattgctaga gattgtaaag aagaagtggt tgtattttgt
    21421 ggagtaaggt ttatgggaga aagtgctaag atattatcac ctgaaaaaac tgtattgatg
    21481 ccagtaagta acgctgggtg tgctatggca gatatggtag atgaagaagg cgtgataaaa
    21541 ttaaaacaac agtatccaaa tgctttggtt gtatgttata taaactctac agctaaagtt
    21601 aaggcacatt gtgatgtttc tgttacatct tctagtgcaa ttaaaatact agaaaacata
    21661 gataataaag aaattatatt ccttccagat aaaaatttag gaggatatat tgcagaacaa
    21721 tttccagata aaaactttat attttgggac ggatattgta aataccataa caatataaga
    21781 gctgaagaaa taattgagtt aaaagataag tataaaaatg cagaagtatt agtacatcca
    21841 gaatgtaaaa aagaaataag agatttagga gattatgtag gtagtacatc aggaataata
    21901 aaatatgcta ctaatagtaa aaacaaagac tttataatag caactgaaga aggtatatta
    21961 catgaattaa agaaaaataa tcctaataaa aacttttata ttcctggtgg aaaaatacta
    22021 tgtacagata tgaaaaagac aacattagaa aatttatata gtacacttaa aaatatggaa
    22081 aatgaagtta ttgtagaaga tgaaatcatg gaaaaggctt taaattcact gcttaacatg
    22141 cacaaattag cagaaggata gagggataga cttatgaatt tagaacaaga tgttttaata
    22201 gttggaagtg gagtttcagg attatactgt gcattaaatt tagataaaag tttaaatgtg
    22261 ctagtagtat cgaaatcaac aattgaaaac aacaatacat atcttgcaca aggtggaatt
    22321 tcaactgcta gaaatattga tgatatagaa tcctttgtag aagatacaat gaaggctggg
    22381 cagtataaaa atagagttga agcagttcaa gtacttgcag atgaatctat agaaaatgta
    22441 gggcagatag tagaatatgg tatgccttta gacaaagaaa atggagagat agattacaca
    22501 agagaaggag cacatagtgt aaatcgaata gttcatagta aggataatac tggagaagtt
    22561 gtttttaaaa ctttattaaa agaagctaaa actagagaaa atataactct tatagaagat
    22621 gcatatcttt tagatatatt aaaagatggc aataaatgta taggagctag aatttttaaa
    22681 tctaaaaaag agattcatgt tttttcaaaa atagtagttt tagctacagg tggaattggt
    22741 gggcttttta aaaattctac taatcaaaga catcttacag gtgatggaat tgcaatagct
    22801 ttaagaaata atataaaaat agaaaattta gaatatattc aaatacatcc tacagccttt
    22861 tatgaagaaa ataatgaagg taggagaatg cttatatctg aatcattaag aggagaggga
    22921 gctaaacttt taaataaaaa taaagaaaga tttgtggatg aacttcttcc tagagatgta
    22981 gtttcaaaag ctatttttga gcaaatggaa aaagataaat taccttatgt gtatttagat
    23041 gcaacacatt tagatagtga atatttaatt aatagatttt cttttattta taatgaatgt
    23101 ttagctagag gaacagacat aacgaaagaa tgtataaagg tatctcctgc acaacattat
    23161 tttatgggcg ggatacatgt agatttggat tcaaaaacat ctatggaaaa tttatatgca
    23221 gttggtgaaa ttagttgtac aggtgttcat ggagccaata gattggcaag taattcatta
    23281 cttgaagggc ttgtattttc aaaaagagca gctaaaaata taaactcagt tatagataat
    23341 gtaaaagtta agtttataga tgtaccagat atggatatag atatagaaca ggtaaaaaaa
    23401 gaaaataaaa tattagtcat aaaagaaatt gaaagaacaa gtgaggattt tggcgatgaa
    23461 ttatttgatt attgatagaa tgatacagga tgctttgata gaagatgttc caagtgaaga
    23521 tataacaact aattctattg tggatgaaaa ttctaaatct actgtagatt taatttgtaa
    23581 acaagatggt ataatagctg gcttaggtgt atttaagaga gtttttgaga tacttggtga
    23641 tgtagatgta aaattatata aaaatgatgg tgatagagta aagaatcgtg aaaagatagc
    23701 tgtattaaca ggtagtacta gaaacttact tgtaggagag cgtgtagcat tgaattattt
    23761 acagagaatg agtggtatag ctactattac aagtaggtat gttgaaaagc ttgaaggtac
    23821 aaatacaaaa ttattagata gtagaaaaac gatacctaat cttagaatat tagataagta
    23881 ttctgttaaa gtaggtggag gatgcaatca tagatttaat ttatctgatg gaatattgct
    23941 taaagacaac catattgggg ctgctggtag tgttaagaag gcagttgaat tagctagaaa
    24001 aaatacttct tttgttagaa agatagaagt ggaagtggaa actttagaca tggtaaaaga
    24061 agcaatagaa gctaatgcag atataataat gttggataat atgagtttgg aaatggcaaa
    24121 acaagcagta gatattataa atggaagagc cattgtagaa ttttccggaa atgtaaattt
    24181 agatactata gaagatatag gaaaaattgg tgtggatgtt gtttctgtag gagcgcttac
    24241 acattctgtt aaagctctcg atataagtat gaagaatttg agaaatatat aaattttata
    24301 ttgaaataaa cttaattaaa atagactcct taaatttgtt ttataaattt aaggagtcta
    24361 ttttttattc attaaaatta ataaaaatag a
//
LOCUS       DACXMZ010000037        24054 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.037, whole genome shotgun sequence.
ACCESSION   DACXMZ010000037 DACXMZ010000000
VERSION     DACXMZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.037"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            79..534
                     /locus_tag="JD016_05835"
     CDS             79..534
                     /locus_tag="JD016_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999154.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(742..1002)
                     /locus_tag="JD016_05840"
     CDS             complement(742..1002)
                     /locus_tag="JD016_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999155.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(1265..1618)
                     /locus_tag="JD016_05845"
     CDS             complement(1265..1618)
                     /locus_tag="JD016_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999156.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(1635..4502)
                     /locus_tag="JD016_05850"
     CDS             complement(1635..4502)
                     /locus_tag="JD016_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999157.1"
                     /translation="MKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKGTHGISV
                     NSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDVGQLGVG
                     DEINRKVFAKINIDNIKSISVNRFTDNSKHAFAIKNDNTCYAVGLNNSGQLGIGDNVN
                     RNVFTKINVENVKYVTVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNIFTRIPI
                     NGVRDVYLCNDVSIIVKNDNTCYVCGFVNGYFGFTESSISTFTKINIENVKSVVTAGS
                     EATFFITNDNMIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTSAILTND
                     NKLFVGGLSGYGSITNNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDTYGISDE
                     YYMDIPVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIHHRYDND
                     KNEALEGSLYSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIFKDNFKY
                     TSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTFTEVNISD
                     VKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVKDISIYN
                     SMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIITTFTKVLTNVKEIKSHNDDHILVI
                     KNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYMITKVDT
                     SKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFEIPQNKI
                     VLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINITENNTDL
                     IVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSYQEIYEL
                     EEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(4839..6959)
                     /locus_tag="JD016_05855"
     CDS             complement(4839..6959)
                     /locus_tag="JD016_05855"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00415.16,HMM:PF13540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999158.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGNGNTMLIDNKGILKVAGNNDIYQLGIANYSENIDSSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTMVILGNGDVYGLGNNGYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLINKSASQYT
                     EISISNVTDAYSAYNATFIVVDEKKVYVTGINKNYMLGVGVNNEAYTANLNLLSTWNY
                     INSIGTSYSRVSCTNDITKINNIVIYEYVTIFCTNIGSFLTGYHGTLWTKPTDSSYRV
                     QYQGISYAGYLDSYIYNYYPTRCT"
     gene            complement(6976..7959)
                     /locus_tag="JD016_05860"
     CDS             complement(6976..7959)
                     /locus_tag="JD016_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAU4999159.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(7960..8658)
                     /locus_tag="JD016_05865"
     CDS             complement(7960..8658)
                     /locus_tag="JD016_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HAU4999160.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(8655..9707)
                     /locus_tag="JD016_05870"
     CDS             complement(8655..9707)
                     /locus_tag="JD016_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HAU4999161.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(9700..10128)
                     /locus_tag="JD016_05875"
     CDS             complement(9700..10128)
                     /locus_tag="JD016_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HAU4999162.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(10128..10454)
                     /locus_tag="JD016_05880"
     CDS             complement(10128..10454)
                     /locus_tag="JD016_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HAU4999163.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(10470..12002)
                     /locus_tag="JD016_05885"
     CDS             complement(10470..12002)
                     /locus_tag="JD016_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HAU4999164.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGTEFSAIFTA
                     YYPGPGIEGGDTDCREKKLNPSKKTCAAPMVGAYEKPYYTKEFLSKHPLFKYGDEVSI
                     VTGVSGRDGLYKVNDNGSAIIIEKDGTYHIDVLVKNAEEMKRFGKRKGKIIIGGYSGN
                     ASNKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKG
                     KKVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARR
                     VL"
     gene            complement(12284..12706)
                     /locus_tag="JD016_05890"
     CDS             complement(12284..12706)
                     /locus_tag="JD016_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999165.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(12706..15159)
                     /locus_tag="JD016_05895"
     CDS             complement(12706..15159)
                     /locus_tag="JD016_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999166.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(15159..15326)
                     /locus_tag="JD016_05900"
     CDS             complement(15159..15326)
                     /locus_tag="JD016_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999167.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(15353..15799)
                     /locus_tag="JD016_05905"
     CDS             complement(15353..15799)
                     /locus_tag="JD016_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAU4999168.1"
                     /translation="MSENGLLKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMCYGKNAVIEEVKN"
     gene            complement(15925..16353)
                     /locus_tag="JD016_05910"
     CDS             complement(15925..16353)
                     /locus_tag="JD016_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAU4999169.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(16367..17431)
                     /locus_tag="JD016_05915"
     CDS             complement(16367..17431)
                     /locus_tag="JD016_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HAU4999170.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(17436..17879)
                     /locus_tag="JD016_05920"
     CDS             complement(17436..17879)
                     /locus_tag="JD016_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999171.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTLTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(18128..18568)
                     /locus_tag="JD016_05925"
     CDS             complement(18128..18568)
                     /locus_tag="JD016_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999172.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(18673..18870)
                     /locus_tag="JD016_05930"
     CDS             complement(18673..18870)
                     /locus_tag="JD016_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999173.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEENIEEVFFIQS"
     gene            19039..19359
                     /locus_tag="JD016_05935"
     CDS             19039..19359
                     /locus_tag="JD016_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU4999174.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            19441..19947
                     /locus_tag="JD016_05940"
     CDS             19441..19947
                     /locus_tag="JD016_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAU4999175.1"
                     /translation="MNNLDKLFELASQEGIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            20111..20527
                     /locus_tag="JD016_05945"
     CDS             20111..20527
                     /locus_tag="JD016_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999176.1"
                     /translation="MYKEKLFDNYFKFLALLFWPIMWYKWIVISNGTLENMLFTIYAI
                     VAIVFIILYSVFMIKYKDITQIDFFYRISTLLAFIFTLFSFLIYPKSLFFLYLKIIFT
                     GIYLYYSIVKTLKFKDDEGVVGIMSSLLLIVITLFY"
     gene            20648..21403
                     /locus_tag="JD016_05950"
     CDS             20648..21403
                     /locus_tag="JD016_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAU4999177.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(21546..21974)
                     /locus_tag="JD016_05955"
     CDS             complement(21546..21974)
                     /locus_tag="JD016_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAU4999178.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(22395..22595)
                     /locus_tag="JD016_05960"
     CDS             complement(22395..22595)
                     /locus_tag="JD016_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAU4999179.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(22778..23932)
                     /locus_tag="JD016_05965"
     CDS             complement(22778..23932)
                     /locus_tag="JD016_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HAU4999180.1"
                     /translation="MKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNMEEKVM
                     ESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYENEVKK
                     MKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQKDKENA
                     FRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGVELQLT
                     DEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGRFKKNS
                     IWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFVGYEKK
                     GRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
BASE COUNT         7584 a         4161 c         2556 g         9753 t
ORIGIN      
        1 tataaatgta gtaaattttt agtttcaagt aatatctcgt tattataact aaatatttat
       61 taaaattagg aggataaaat gggcttaaga gacaaattcg cgcaatcatt tgctagatca
      121 aaaactatga gtggccctga gaaaaaggct aatgaaataa tgggtaaatt gcttcttaag
      181 aaagctatat tacctatcgt tttaatgttc gtgataataa tcgctggagc tatgcttaaa
      241 atcaattctt gggttacact tggcataaat ttagttattg ctgttggtgc tttcttctac
      301 ataagaaatt ctagtaaaaa gtatcagaac tttaaacctt atgttggcaa tttgattagt
      361 ttagagaaaa aaggtaaaaa agagtacgtt gctattataa aacaaggtaa gttaccagtt
      421 aagttacaaa tagcttatgg tggtgaagac cttgagcatg ttaagaaaaa tcaaatggtt
      481 caaataagtt ataatccaga tgctaaaata gctattttgg ttaatagaca ataatttaat
      541 aaaaaaaagg agttgtctta aaattgattt tttctaatca aatttaaact caactccttt
      601 tttaattttt atatattatt tttattactt tttttaatta atatttttaa atattaaatc
      661 cttctcaata aaatagtgaa ctatctacaa tactacttta tcttttccta acaatatata
      721 atctggtgct actctttcat gttatacacc gttttgtaac atacctttta tatcagtaac
      781 atcttcctta atttcctcta catctgtttt cattattccc atttgaacaa gtatattttt
      841 gtttatttct tgttgttgtg tagataattc tataaaattt tctacagttt ttttatacat
      901 gtctctatct tcttttttct cctgcatagt atttttaaat aatagagcac atagtatacc
      961 tattgcccct agactactca attcggttat taatttatcc attatagcct cctattgtct
     1021 aaatttttta tacttatagc tttaattttt gttgtattct tacatcatgc aatattatat
     1081 tttcaggatt atcaatttaa tcttctgtac tttaaattgt aggtgtaatt tatagattga
     1141 ataaataata aatatatata caatttctaa tataacaaaa aataagtaca aggcttactt
     1201 attaaagtta agaccttgta ctttacataa aaaaaataac tagtatatag ttattctaaa
     1261 tttactattc aactggtatt tctaattctt tacatctaat tctaacttgc tctctaatta
     1321 ctgctggtac ttcttctatt gtttttttgc ctcttaaaat taaagctaca tatacttcta
     1381 ccattccact ctcacctcca gattttctta cactaaaagt gcttatatta cttgcttcat
     1441 tgacagaact ttgatttaac aacatctcat atatttctgc atttgctata aaattatttg
     1501 ttatatcatc atcttgcttt acttgaccta tacttatgtc tgagatatct ttatactgca
     1561 tttctacttt tctaccttgt ttgatttgtt ctattttatt tttatcgaat ttaaacaatt
     1621 ttcacacctc cattttaagc ttgtggctcg ttgttttcag catcatatct aaaaatcata
     1681 gatggacgtt ttatagctgt ccatttatct cctttttcta ctatgatttt aggctgagca
     1741 ctttttatgt tggcttcttc taattcatag atttcttggt aacttgagtc tgattttttg
     1801 atttcaacta gtttcatgtc tttgaataag tcagatttat ttatttttac ttgaacttta
     1861 gttttattta ttaattgata tataggattg ttttccatca tgtcatcctt taatccctca
     1921 tttaatgtta ctattaaatc agtgttattt tcagtaatgt ttatgattga atatacttta
     1981 ttgtttatca ttaaaataga atcctttttt actttaagcc ctgtttctga tttaaatata
     2041 aacatacttg aatataaatc gcctgtagga ctactggctt taatcagtat cttattttct
     2101 cctaatacta ttttattttg tggtatctca aatataatgc tatttatttc caaattagtc
     2161 ttagtagata ttaattcatc atttataaac atttcaagtt tttctataac attattagga
     2221 tcttgtacta caactctcac ttgagatatt gatttttcaa ctatttctat agatgcttta
     2281 cttgtatcta cttttgttat catgtatatt gtatcatctg aaagctctat atctaaaata
     2341 tcatttagat gttcaggaat tggtatttta gtaaattcat ataaatcagt aattgatata
     2401 gatattttat acatagattt atttttacca gttatccata gactattatc atttttaatt
     2461 actagtatgt ggtcatcatt atgtgactta atttctttaa cattagttaa tactttagta
     2521 aaagttgtta ttattttatc tttttcactt tctcctagtc caaacaatcc atttgtatta
     2581 tacccttgag cgtacaactc tccctcatta gttaaaacaa gtgtcattga gttatatata
     2641 cttatatctt taacagaagg taatccagta accttttgta actctcttac atcattattg
     2701 tgaccaactc ccaattggta ctcagtattc tttccatatg accatagagt attatctgtc
     2761 ttaataatat aggagtagtt atctccacaa gcaacttttt tgacatctga tatatttact
     2821 tcagtaaaag ttaataattc tatatcttca agagtttgat taatttcttt atatgaatta
     2881 cttcctattc catataattt tccatcctta gcaacagcca atgaatgtgt ttttgatata
     2941 tcaatatcag atacctcaaa tggcaatatt attgaagtat acttaaaatt atctttaaat
     3001 atgtttttat aatttatata cttattattt gtttttatat acttatcatt aaacattact
     3061 agcttttcat ttttcttatt aaaaactatt ttatctgatg tgttatctgt tttaaaatat
     3121 gaatataggg aaccttcaag agcttcattt ttatcattat catatctatg gtgaatacct
     3181 aaatgattat atctgttatc tcctgttgaa gaataaaatt ttccatatat atctttaata
     3241 aatatagtat tgtaactaga aaatacagta tcaacatctt tctttataga tactttataa
     3301 tatggaactg gtatatccat ataatactca tcagatatgc cataagtatc tctacctgat
     3361 gatatcaaat tgttattatt atctatataa agtgtattat tagctgtcac aaaaacatct
     3421 ttaacatctt ccacactatt tgtattgtta ttatttgtta tacttccata tccactaaga
     3481 cctccaacaa atagtttatt gtcatttgtt aaaatggctg aagtatatcc attaactact
     3541 attttttttg catttataat attattaatt actcgtatac cctttatatc attagtttct
     3601 gttgaaaaga ataccctctc ttttttccct gtagtataaa tcatattgtc atttgttata
     3661 aaaaatgtag cttcacttcc tgctgtcaca acagatttta cattctcaat atttattttt
     3721 gtaaatgtac ttatactact ttcagtgaat ccaaaatatc catttacaaa tccacataca
     3781 taacatgtat tatcattttt gacaatgatt gatacatcat tacatagata tacatctcta
     3841 acaccattta taggtatacg tgtaaatata ttcctacttg tagtatcacc caatcctaac
     3901 tgtccttttc cattattacc tgctccatat aaaagaccat cattagttaa taatagagat
     3961 gtgtttccat atacagttac atattttaca ttttcaacat ttattttagt aaatacattt
     4021 ctatttacat tatctcctat tcctaactga ccagagttat ttaaaccaac agcataacag
     4081 gtattatcat tttttatagc aaatgcatgt ttactgttgt cagtaaatct atttacactt
     4141 atagatttta tattatcaat atttatttta gcaaatacct ttctatttat ctcatctccc
     4201 actcctaatt gacctacatc atttaaacca acactgtaaa gcatattatt attagttaat
     4261 aaatatgtag actttgaatg gctatatatt tcttgtatat tagaagatat ttcactactg
     4321 ctattctttt tattgtaatt tatcttatta tttaaattta aactactatt aactgatata
     4381 ccatgcgtac ccttagagtt atatccacat ataaataagc tcttatcagt taatattgca
     4441 aatctagttt tatctctact taaatcataa ggaggtataa atatattttt tatatttttc
     4501 attacctttt tatagttagt tgcattatat ccaccttgta agtctgatac atctagagct
     4561 tttgcacttt cagatatatt cccaaaacca tatagtgtat tagaggatag atataataat
     4621 gctttatata aaagtatagc tttgtcagag gtaataggac ctgaacttat attagatgat
     4681 gcaatattac tagatgatag attattaaag taattccttc catattgatg tatataagat
     4741 gaacctccag aaataagtaa attatctgga ttgacatttt ttaaattact tgatgattcc
     4801 ccattatata aataagcaaa agttgtagaa gatgatgatt atgtacatct tgtaggataa
     4861 taattatata tataagaatc aagatatcct gcatatgaaa ttccctgata ttgaactcta
     4921 tagcttgaat cagttggttt tgtccataaa gtaccatgat atccagttag aaaagatcca
     4981 atgtttgtac aaaatatagt tacatactca tatatgacaa tattattaat tttagtaata
     5041 tcatttgtgc atgaaactct actataactt gttcctatag agtttatata attccatgta
     5101 cttaataagt ttaaatttgc agtatatgct tcattgttta cacccactcc tagcatatag
     5161 tttttattta taccagttac atataccttt ttctcatcaa ccacaataaa tgttgcgttg
     5221 tatgcactat atgcatctgt tacattgcta atactaatct cagtatattg acttgctgat
     5281 ttattaataa gtccattaac ataacttgaa ctctttccag aagtatatat ttgcttatta
     5341 ttagttacaa ttaatgaaaa ccaatttgat aaatcagtat ttataacaac tttttttaca
     5401 ttatctatag atattttctt aaatatatct gaattagaag gaagaacacc tgctattgag
     5461 ttactatttg gtccagaata ataacaagta tcatcatttt taatgtagaa tattcctcca
     5521 gaaatatttt ttgatgctac tatagacttt acattagaga tacttaattt tgtcaattca
     5581 tttaattgac ttggtaagtc agaacccatt cctttatatc cattatttcc tagaccatat
     5641 acatctccat tccctaaaat aaccatagta caataaggac taaaaacgac atctcttaca
     5701 gaatgtatat ctagtggaag ttcaatatcc tttatttcca tttcttcttt taataaagtt
     5761 aatttatcct cagatttaat taaatttata taattactat aactatattt ttcactatcc
     5821 attttcttaa attctacaat gtgcatatca tcaaaaaacg aacctaattg tccataagta
     5881 ttatctcctg tacaatatga atcattattt gaatcaatta gtatgctatg tgataaacca
     5941 atgaaaatat tcttagctac aatatttttt aaatctataa atgaactatc tatattctca
     6001 gagtaatttg ctatacccaa ctggtatata tcattatttc cagcaacctt caaaatacct
     6061 ttattatcta taagcatagt attaccatta ccacatgcaa tagtttttat atttacttct
     6121 tctatcttag taaatagtga tgattgaagt gcatttccaa aacatttttc ctttccacaa
     6181 ccatatatat cattattata cgtcattaac acagtgtgtt cagaaccaca agctatagtc
     6241 ttaacatcat taatagaaac taaactaaat aaatctctat tattcttatc tcctaaacct
     6301 agttgacctt gattattaaa accagtagaa tagcatttat tgtcattctt tattataaaa
     6361 gctgatttat tatatgaaaa tacagccttt acattgtcaa catctacttt tgtaaatgta
     6421 tttacatttg cagatgctcc attagttcct agacctaatt gaccatacaa attatttcca
     6481 gcaacatata aagttccatc attgtataaa agatatgttg attgaagtcc acatgcaata
     6541 tcctttacgc cttttatttc tggaaccttc tcaaatgtag aaactgtttt ccctactttt
     6601 tctaatccca atcccatttg accatctgta tttatacctg cgacaagtaa ttctccatct
     6661 gaagtaagta taactgcatg agattctcca catgcaactt ttttaatatt ggtagatata
     6721 tttggtattt tcgtaaataa ttttcttgtt gtattgtctt ctcccaatcc aagttgaaaa
     6781 ttactattat ctccacatcc atacaagata ttattatttt ttagaccaaa tgtatagtta
     6841 caacatccag taaaaaaatt aataaagtca taattatcat acccttcatc aaattcatca
     6901 actaccatat ttttatcgcc aaagaaatta cctctttgaa gtagtttagt tcttttcatt
     6961 ataaaaacct cctaattatc ttatttcata cgaatattta tttaaagtta tatctgtctg
     7021 accaattgaa tatgccctat tagattttgt ttcaacaaag ttattatcca tttcatgtat
     7081 atatctaact ctatttgcat taaatggtag caccacattc ttaaatgtaa cactaccaag
     7141 ttctccagtt atacactttc tatttaaatc taacttaagg ccttctatag ctattatgtt
     7201 tttagcatta gttaaggtat cataccaata tccagcttca tctacatcta tttctgactc
     7261 taattctaag tatattaaga tatctaatac tttttcagat aatttattgt aactattttt
     7321 taatacagat agttcagttt gtatttgacc tcctattaca tcatcaatag atatattttt
     7381 aattttttct actacctcat ttatagctcc tgataagttt ttagcttctg tagataagag
     7441 ttcttctcca atttttactt ttatttcttt tatattttta tcaaacttat catctaaatc
     7501 ttgaatatcc ttttgagtta caaaaaccaa cgtcgggtct acttctaatg taacatttga
     7561 agtatttgac aattgtaaaa tcatttttac aaccaaatct ttaatactgc catcttcagc
     7621 acgtggctta tacgtctctg gatacttacc tatagctatt atattattct cattatccag
     7681 aacagcagct tctcttatca taaatccacc tactgttcct ggtaaaatag tctctataac
     7741 tatccaatta ggattttttt catcaattct tagagaattt atatttcctt cccaaactac
     7801 attctttaaa gcagtttgtt cctctgttgg ctcataatat tctccattac tatctccaac
     7861 ttgaaacttg gttaatccaa caagctctcc tagtgcagtt gcatttgcta ttttttctct
     7921 accaattttg gttaatatta tataaaattt ttcttccaat tataccacct ccgatttagg
     7981 ataaactact acattttcta cacctgtatc atttgcaata gctatattaa tcttacaatt
     8041 attttcaatg catttaatgc ttaaaggata aacctttatt tcttctctac ttaacattcc
     8101 agctccacag tttacaacag cactacattt tataattggt tcaaagtatg gatgaacttt
     8161 tacctcttca ctacatacca tacctccacc acaatagacc atacttttat ctagtattat
     8221 tggttctaaa tagtgtaata agtgagcagg ttttactctt tcaatcatat cgctacaatc
     8281 taaaagagtt ttatagtcac agttatttgc tataaaactc aatacgaatg taaattcatc
     8341 actataaact tttatatctg tttctgattt tgtatatgcc tcacatatac tttttataac
     8401 ttcaatagta ctagtacctc tgcttctcat tttagctttt atattgctac gtcttgtgtc
     8461 aaaatcaagt tctttttttt caatgcagag tatgtcttcc cacatatcta atccccatgt
     8521 tgcagtgtca acaaataatt ggttaaaggt atcatcaata tcttctttaa gaatatttaa
     8581 ttctgtatcg tatgcacctt gtatctcctc tacaatgtaa tttctatcaa atgatggtag
     8641 tttatcaatt agcttcattg attttccacc tcactaaaat taacagttgt tacactaggt
     8701 attttatctt cattaatagt tatattatct gtacttccat ttataagtag gttacttaaa
     8761 tcgtgtacac cagtagtatt tataagtatt cccattactt ttgtatagat tatctctcct
     8821 ctaatatctc taaagtatgt atttatactt tctaggaaag attcttttac attgtctaat
     8881 gtatatccat cttctagttt cattactgca cttatactta tttctattgg taatggtgtc
     8941 acaactgtta tagttggtcc aataggcttc tcttcatcta tatgttgctg acacctttca
     9001 attgtttctg tatcaacagc ttgattattt ttcccaaata tcaagacctt aactgttccc
     9061 ggaccatccc atcttggata aaccttaaca ttatagactc catctacttc caaagcccat
     9121 tcttcatagt gagctttatt tccacttgta gcttgatttc tttggatttt ataaaatctt
     9181 tcttttagtt cttcatctgt ctctatatct gtaccacctt caaaacttct tgtgttagta
     9241 atctttgtta ctccagatat attatcaact agtttaaatt cacaatttgt aggtaaatta
     9301 tatttcttac caacttccag agcttgaact gggctattgt caccttcttc actaccaata
     9361 gttacatctt ttattactac aaatagtaaa tctctatatg atattattgt gccattattt
     9421 attacagtac ctttctctcc aataaattca acttctccat ttgactctgt acctaacttt
     9481 ctatatacac caaattcatt gactctttta tcaagaaact ggttatatgt gtcttgtata
     9541 aaagccattt tatgcatctt tgaaagttct agatatatct ttgaaagttc tagattattt
     9601 ccagatacca tgttgtttag aaaagaacct tctcctttat aaatatcaag attaatattt
     9661 tcaagagttc tattttttat tacttcatat gtctggtcac tatacattta tttccacctc
     9721 cccatagata gtggatactt ttacatttgc actcaatacg tctctgttaa agttagcact
     9781 ttttacgttt acttctaata tatatggatt tattagaagg gcctctttta tgaatctact
     9841 agcttcactt tctgtaagac cttttgtata tttttgtcct attagttctg aaagctctgt
     9901 cccatattct aaagagtata tctcatgttc atatctattt gtctttatac acttgtatat
     9961 ccaaactttt aaggcttctt ttttttcaat tattttaaag tcaccttttt ctaaaatagg
    10021 ttcatctttt tcaaaatccc atgccacttc acgaaagatt ggcaattctt ctgttttagg
    10081 taagatataa tcctctggga cacctataaa aggaaatatt gtactcatta taagctcacc
    10141 aacttactta caacagcaaa tttttcgcct attctaaaca ttattaccat atccccagat
    10201 tcaaaattat ctataaaagg attttttact tcatgctggt gttcttgatt tgtttctgtg
    10261 tcaaatgttt ctagctgtct atcaatcatc caactatcta tcaaaatatc ttctttttct
    10321 aatatgatgt tatttgtctc tatttttaaa tctggtaatt tacttttaat ttttccaata
    10381 aaaaaagaag gttgattatg atactttcct tcttctctta ttattcctat aaattcattt
    10441 attggattag ccattatatc accatccttt tataatactc tcctagctgt attaaaatct
    10501 cttctactac ttaacttact tattttaact acatcacctt tttgtggagc atgtaaaaat
    10561 tctccattac ctatatataa tccaacatgg ctgactggat tatgaaaaaa tactaaatct
    10621 cctgcttgaa gatttttttg ttctactttc ttgccttttt tagattgttg atttgacgtt
    10681 cttggcaaac taacattaac ttttttaaaa caatagacca ttaaaccaga acagtcaaaa
    10741 ctacttggtc catttccacc ccatttataa ggtttaccta gatgtttttt tgcttctgat
    10801 attactattt tagctttatt agatgcatta ccagaataac caccgataat aatttttcct
    10861 tttctttttc caaatctttt catttcttct gcatttttta ctaaaacatc gatatgataa
    10921 gttccatctt tttctataat tatagcagaa ccattatcat ttactttata cagtccatca
    10981 cgtccagaaa ctcctgttac aatacttact tcatcaccat atttaaataa tgggtgtttt
    11041 gataagaact cttttgtata ataaggtttt tcataagctc caaccatagg tgcagcacat
    11101 gtctttttag aaggattaag ctttttctct ctacagtctg tatctccacc ttctatacca
    11161 ggtccaggat aatatgctgt aaatatagca gaaaattctg tccctcctgc ataatcttct
    11221 ccccctaaat tactttcttc cttaggttca tcctgtcctg ctgacttttc atccataagg
    11281 ttttgaaaat taagttcaag ctcaatttga tactctccat tttgccaagt atgtttgtct
    11341 gtatctatat aaaatagtcc tacaagcttt gtataagaat ctttaacttt tactcctcta
    11401 ccagttatac aacttacatc tccataacct ttaagagaac agcttttttc tatcccatta
    11461 aactcgctat caatatctac atcttgattt tcttgttgct gaattacttt ttgcattatt
    11521 acatttactt ccttaaaaat ttcattatct attttttcgc taatcttgct tccatactgg
    11581 tctaccacta ttactttatt ttttacattt tccatgctct ctgaaaaggt ggtatttata
    11641 atattaaatc cctcttcaaa cataacactt aatgtaacag ttcctttttc aataacatta
    11701 aacttatcta aattggcctc tatcatatac tttttctttg tctttttact tgcttctgta
    11761 tatgcactca ttattgtgtc ataaccattt actcctataa acatctttgt atacttgaca
    11821 ttggtctttg ctattgttcc aactgaaagc ctattttcag caaatacttg ctttgctatg
    11881 tcttcaacta acttatcttt aaaattgtat gacacttcac tttgtgtaag taaaaatccc
    11941 atatctttag atacaaaact aatttcatta ctgcttgaat ctttagacct attaattatc
    12001 attcctctaa agagttcttt ctcatctaca tagaaacaga ctgtactagc tattggtata
    12061 tcgatttgtt ggaaatttac atcaaatgat gattgtatta ttgaaaactc tagtgtcctt
    12121 gatggagatt tataatcacc tgaccatgat accttgtcta ctatgtcagt tatgtcatat
    12181 atacttccgt tttttatgtg tacccaaatc ttaataataa aaaatcacct gccttttcat
    12241 ttaattctat cagcagttat ttataactta ttatctataa tagttattgt tttggagtta
    12301 cttttggtat ctttattctt ttatactctt ttaaatctaa tgtaaaatat acatctcgag
    12361 tcccatctct ttcttcataa ctaaaccctt caattatgac ttccatattt atatttgttt
    12421 ccgtaattgt aaatcttaat ataaaacctt ccttcatcca tttttctatc ttatttacac
    12481 aatcatatgg tgatggaaaa cctgtatagt cacagaaagt atagtcttta tttggaaaaa
    12541 aacttgatat tgaaatgctt ttaagagcta caccaccaaa agttgctatt tcacctattt
    12601 tgagtatact agaagagttt acagcagctt ttccattaat actaaaggaa gatggaaata
    12661 ctggaaatct aaatctatct tcagcttgtc taagccacat ttccattata agacaccccc
    12721 taactgtgcc aattggatac ttgcaactaa ttttgatgtt atcttttcta tatcagcttc
    12781 ttctctaatc actattgtat cagctaactt agctatgttt atatttccac cattttttct
    12841 gcttctatat tgatttgctt cttgtttagt taaaaccatt tcaccttcat ggagtctggt
    12901 ttgatagtca ttataaggaa catagttcaa tccaaatgca ttattacctt tcttacctga
    12961 ataagcttta ccagaatcac cagcctttgt tccagcatat ggattatctt tgtaattttt
    13021 tttactcttt tccactagat ttaaaactgt tcctgtgatg cttccatttt ttatcttatt
    13081 aatcatagtt tgccaccact tgcatatatc tttaactatt ttacatactt tatctaatat
    13141 gttaaaaaaa gctcccaata ttggctctat aattttccat ccagtttcca acagaggtcc
    13201 taaggtctcc catacagttt tccaaataac acccagtgtc tctataaaat tgtttatttg
    13261 aggtgcatat tcacctaaaa aagtaaaaat tcttgtgata ataggactaa cagcttcaac
    13321 tacaggtccg attagttgga atgctgtagt tactattgta ccaaatatac ttattaatcc
    13381 ttgtgcaatt ggtgatttat tgtttatact atctagtaaa ttatttacta agtttactac
    13441 tggaagtaat gaatctatta atgctgtaaa atcgatattt tgtaatagtg tacttcctgc
    13501 gttaacaatt gcttttggtg cattccctat cattgttgct gcatgatttt caaaacgttc
    13561 ttgcttattt tcaaccatca ttttacgctt ttcagcctca ttagaaattc gatattgagt
    13621 agaatcagaa aactcttttc cattttttac acttctagat tgcatctggt catatttgtt
    13681 gccaataatt atcttaccat tcattacatt gcctatttta ccagccattt tttcagataa
    13741 gccagctaaa ttgcctaatc cactttctat aaaagttttt attttcttta gtccttgacc
    13801 taaaatatca tcaaaaggtt taacaatact cttcaagcca ttgttaatgc ttgtttgagc
    13861 tcctgatatt atacctggta ttgtattact catttcttta gctaacccca tctgctttac
    13921 acttatgcct tccaatgctt catcaaaagt tttataattt tctcctacca gagtattaag
    13981 agattccatg tttccttttg ctgcacttaa gaaagctgct gatacatctt gttcactact
    14041 tgtattcata tttaaagctc taacatctac catagcttga gttatatctt tttctttttt
    14101 gccaccagta atattactaa tcattttagc tttagttccc attgcaacaa catcttcagg
    14161 gtcaaaaggc gtatcatttg ccattcttac taaactgcca taataatcat caacatctat
    14221 ttgattaact tttccagtat ttcttttcat tacactttct agaacaactt tctgattttg
    14281 ttcctgacca agtaaatcaa cacccttttt cacagcgaca cctatagtag caccatttgt
    14341 caaatcagat atcttccctt ttatcgaacc aagtattcca cttgccatat cctttacagc
    14401 tattgcacca ctccatacct tacctgcaaa taatctcaat ttgggaaata ctttacttat
    14461 aatgccactt gccatatctt ttatagctat tacaccactc caaaaagtat ttttaaacag
    14521 cattaactta ggcaaaattc tacccatagc actactcacc atatctttaa gattaatact
    14581 tccttgataa ggacgtttct ctacttgttt taattttccc aagatactac ttatcactct
    14641 cttagtcata tcttttgctt ttattacgat attccaaatc ttgccaccaa tcttttttaa
    14701 atttccaagt aatttatttt tagttttttc tacttcatca cttttagctt taaatattaa
    14761 tataatcata atatttgaat taaactcatt tttcttttga atcaacttat taaaataatc
    14821 aataatcata gctatagtta ttgctagtct cataatactt ccatttaaac ttccaataac
    14881 tcctgaaaca gcatttatgc tagaacttaa ttttgtcata gttgaactta cactactagc
    14941 agtagtttga acttttctta acgaggaatt taaactgttt actctattac ttgcacttcc
    15001 aatagaattt tttatgctac tagccattac attagtggaa agtgaaacag ccacagttgc
    15061 tctcctaaaa ttatcagtta ctgatataat ttgattcatt tttggtgtgt aattatctgt
    15121 aaggtacata tttatacctt cttctctcac atttcccatt acataccacc tcctgtcaaa
    15181 ggacacactc catatccatg tttcattttt tcaacttctt cctcaatttc taaggcgaaa
    15241 aaagcttcaa taagctttaa ttcgccttta tccattgcat aaaaaaggga cggtcttata
    15301 ccttttttct tccaataata gtacattatt gtagtcctgg tatccgtccc tattagtttt
    15361 ttacctcttc tatcactgca tttttaccat aacacataac atcttgaagt attctatata
    15421 agttggtaat ttcacctggt aataacaatt ttcttgctaa ttcttttggt gttggagcct
    15481 taaatttatc cattagctcc ttatttttaa ataaaagctt tccatccaaa tcacaaacac
    15541 cttctataat tgtaagcatc tgcatctccg gtaagtctat atccgttttc atattttcat
    15601 caattttaag acaagtattt tgtatctctt catctttact tattgttaaa gctctacaca
    15661 ttactttaaa ttcctgccca aatatagttg ataatctctt aagttcaact atagtacttg
    15721 gtctttctaa gttttctgta tctgcattta aaagtaaatc tactatgttt atatttttta
    15781 ataatccatt ttcactcatt attatttgct cctttatcaa tcaaattcaa tcaactaact
    15841 tttttactta ttaactatct gaatttatta agtatatatt tactaaatat cagtataaaa
    15901 ccaaatataa aactaaatat aaaactaaat tatatcaaga aaatcatact cagtaaatgt
    15961 gaaaggtgct tctatctctc cttttacgcc aacctcccaa tcagctaaag ttaaatcatc
    16021 aaatgctata ttctttactg ctattctttc tgctccataa gaatctgggt catttaattt
    16081 agatatagca acaaatctag gttcagaacc tctctttatc ttttctccaa ttaacttgtg
    16141 cattcttgaa ctaacatggt atagagttat tgagcctttt cctttatatc ccatatactt
    16201 tgtatcagta cccatttgac ctgctattat aatatcttct tttgtaaatt ccatctttgc
    16261 ttgaaacttc tttacttctg ctactttgtt tccatcaagc caaagttctc cccaagtacc
    16321 actcattaca tttctagctt ccatatttgc catattaata atcctcctat atttctattg
    16381 ataaatctat atcttccata gcatcaagta cttttatttt tgcttttaaa aatactttag
    16441 aacctgtgtt agcttctttt atttcttgta atgtcatata agataaatct actccttttg
    16501 attttaaata cgatttttgt gcttcaaaat ctatttcaac agtagaatca gattctataa
    16561 gtgctgattt ttctaattct tctaaataac tttttatagc tactatcaat aaacatttgt
    16621 tgtcataact gttagtaacc tttcctatat agtcatctat tatcaccttt cttatgtcac
    16681 tatgtataat atctaaagtg tcaactattt ttattttctg gaacatttct cctttttctg
    16741 ctgttaattc agttaaagaa tttactcctc tagcaattct tatagctcct gcttctttaa
    16801 taagtataag ctctccttta ttaacctttg attctgcatc aactttcgtc atcttaggta
    16861 tatcgactac atcactaagt ttagtataag ttactgattg acttaaaggt gtacctgcta
    16921 taagtccagc caccctactt gtaaactcat caacactgta tttcttttct ccaactaaca
    16981 catcttctgt agtaaaatta attatccctt catgatttcc tacaactttt cctaatacag
    17041 ctttaacctt aaccttatct atatctctaa gtttaattat ccaatttttt atagcagtct
    17101 tatcagcttc tactgctttt ggcatacata gataattaaa ttccttagtc tctaaaaaat
    17161 ctaatgcagt ttgaatatct gcttctcctt ctattacata aactaataat ttatttggag
    17221 tgttaacatt tcccatcaag gctaaattta tatattcttt attttcagca gataaatcaa
    17281 ctggtatatc ctctttttca tgtatttcat taagacctag tgccttagca tctttaagca
    17341 ccattgcaat tattcctcta gctgaacgtt ctttaacagt tgtagctagc tccttaaatg
    17401 atatgttgat acttggtaat cctatagcca tactatcact ctcctaaatt taaattgatt
    17461 tcttccatat tttcatatac tacatcttct tcatatacac tatccagata gtttagtgta
    17521 agtgtaaagt tcaatacata aattgactct tcttttttaa tactttgttc tagctttgtt
    17581 atatttaatt ttctgtctgt tacctttata tttctgttaa atagcttatt taattcatct
    17641 gaaacttcat acattctaat actctttgac tgtggttttg gtaagtattt tatagagata
    17701 acaatatctt tgttattcgt atttaacatt acaggtgtac aaataactga tagtatactt
    17761 acaaaaaaac aagacccttc agagccttgc acactttcat catcaataaa aatactttca
    17821 gtaaagtttt ttttgagaat ctcaataatt gtttctaata tttccttgta tttaagcaaa
    17881 taatcaccac actatcacta aatattcttg cttaatctga taatcttact ataaatgata
    17941 taagtcattt ttaaatatat atctcattta ttcctgtcaa gtatcttcta tatttttagg
    18001 taatattatt cttttcaatc tatcacctcc ttcaatatgg atttctttca caataacatt
    18061 atctcacata ttttattgcc tattgttgca atggtgtata taaagtgttg taaaattgtc
    18121 ctatttttta taatatctca ctcaatttaa atatcatctt tttctttaaa ttgtagcaag
    18181 atgttctatc taagtgtagt ttcatagaaa tatatgtcat attgtttttt gtcttactat
    18241 tataaaaaag attaaaaaat tccatttcta tatcatttag acacgttaga gcattttcta
    18301 tcttctcttt ttctatttct atatcttctt tttccatttt taattttctt aatctttcct
    18361 ctctttttaa cacctcattt tccacagaag agcttatgtt atatgtgtta ctagtctttt
    18421 ctgtatatac cattgctcca catccagagt attcattctc acatcttttt atgtccaagt
    18481 taatattctt tatctttata tccaaatact tataattatg taatctttta tctgtttctt
    18541 taaataatat atcttttttg ttttccatag tacttctcct tttttatata cttattatta
    18601 gcttcaaatt tacaataatc tctttagtgc acagtttttt actaatctca caactttcaa
    18661 gaacatattg ttctatgact gtataaaaaa aacttcttct atattttctt ctaaaatact
    18721 agaaatcttc ttagccataa gaagtccagg attttttatg cctctttcta tttgagatat
    18781 ataatcattt gtaattccag cctttctggc aagttgaatt tgtgtcatac ctttgcattt
    18841 tctataatat ttcaagttat ttttaaacat tgttttatac ctccattact attaagaata
    18901 ttttgttctt cctcacttag attatataga attatttgtt ctttatcaat atattttaga
    18961 aaaaaatatt cttttatatt ttattagttt tattttttag aacaatttgt tataatctat
    19021 acataaggtg gtgttataat gtttgccaaa agattaagag aacttagaaa ggaatttgga
    19081 ttgactcaaa gagaacttgg agaaaaagta ggcgtttccc aaagagtctt agggtactat
    19141 gaaacagaaa acagatttcc tgatgaacac attttaaata aattggctga tgtatttaat
    19201 gtatcagttg attaccttct tggaagaacc ttagttaagg aaaatattga tacagtagct
    19261 gcacatagaa aaaatccaca tgaagagtta cctgaagaag cacaagaaca acttaatgat
    19321 tatatagaat ttttactaaa taaatataaa aaaaaataaa ccatatgatg agcagtctat
    19381 ttttctgctc ttttaatata atttaaaggc aaacatacat tctattttag ggggattttt
    19441 atgaataatt tggataagct ttttgaatta gcttctcaag aagggataat aattcattat
    19501 accacttata ttgcaggtga tttagaagga ctatatataa acaaacatgg tattaaaatt
    19561 atatcattac tcagtaattt aaaacaaaac tcaaaaaaat taacatccat cttagctgaa
    19621 gaacttggac atcatttcac tagccttgga tactatgtat cctcttataa cgattattat
    19681 acaaaaatta ttatagataa gtgcgaaaat aaagccttaa aatgggcttg tgagttctta
    19741 attactgaag aagatataat aaacataatt aactctggca tcacctgcgt ttatgaaatg
    19801 gcagacatac ttaatgtgga tattactttc tttcaaaaaa gattagaatt tttatcatta
    19861 aaaaaacagt ctttacaact tggaaataat aaatatttaa tattaactaa tttgccgtat
    19921 ttctacatat ttgatccaat ttcataataa aaatagtagt aattttaact ggcttaacag
    19981 taaaatctat atatttattt taacaaattt tggtttctat aaattcaata ttaatatata
    20041 ataatgatat catattattt aaaattctaa aaacaaatat atgcttttgt taagaaaggt
    20101 tgtttgatga atgtataaag aaaagttgtt tgataattat tttaagttct tagcactatt
    20161 attttggcca attatgtggt ataagtggat agtaatttca aatggtactc ttgaaaatat
    20221 gttatttacc atatatgcaa tcgtcgcaat tgttttcatc attttatatt cggtgtttat
    20281 gataaaatat aaagacatta cacaaataga tttcttttac aggataagta ctctacttgc
    20341 ttttatattt acacttttta gctttttaat atacccaaag agtttatttt tcttgtattt
    20401 aaagattatt tttactggaa tataccttta ttattcaata gtaaaaactc ttaaatttaa
    20461 agatgacgaa ggtgtagtgg gtattatgag ctcactttta ttaattgtaa taactttgtt
    20521 ctactaaatt taattctatt tgacttaata tggctttaaa tattagacat ctacattatt
    20581 ttctgatatt atttaagatg gtagtcttat attaataatg aaatacacta tgaaggagtt
    20641 aactattatg gaaaaaaaag ttttagcaac agttggagaa aaagagataa ctaatattga
    20701 tattgaaaat gcattaaaat cattagaccc ttatcaagct atgcaattta aaactgaaga
    20761 aggaaaaaaa cacttattaa atgatttagt gaatcaagaa ttattcttct tagatgctaa
    20821 agaagaaaaa ttagatgaag aagaaatatt taaattagaa atgaaaaaga tagaagaaaa
    20881 tgtattaaag caatttgcta taaataaagt tttatctagt gttaatgtaa cagaagatga
    20941 aaaagtaaaa ttctttgaag ctaataagag ctctttctct aaaccagaaa gtgcaactgc
    21001 taaacatata ttagtagata gtgatgaaaa agctaaagaa atacttgctc aaataaaatc
    21061 agaagaaata tcttttgaag atgcagctct taaacattct tcttgcccat ctaaagatat
    21121 gggtggtgat ttaggaacat tcggtagagg acaaatggtt cctgaatttg aagaagctgt
    21181 gttctctatg gctaaaggtg aagtaagtga accagttaaa actcaatttg gataccatat
    21241 aataaaatta gaagaccttc aagaaagtac agagtctaca tttgatgaag ttaaggctga
    21301 agttgaaaaa agtttattat atcaaaaaca aaatgaagtt tatggaaata agataaatgc
    21361 tcttaatgct aagtacggaa accttgtaaa atacaacgat taatcataag gtcatatata
    21421 aaaaagctgt cttttcacaa tatttattag aaaagacagc tttttatatt aaagtttaaa
    21481 tattcagttt catattatca ttcttttatt gtaaagaact cagcttaaaa gtttaaataa
    21541 gatttttaat tatcattagc taaattttta tatccacgta ttatctcata acagtaacct
    21601 attacatctc ttctttttat tatcccaata aatgtgtctt ggtcatctat aacaggaatg
    21661 aaattttgat ttaaagattt tataactaaa tcttcaatat ctgcgttgat ggaaacagga
    21721 gaattatcca tttttctatt tatatctgtc actggaacat cttccaatcc ctttaagtcc
    21781 agatttaaat cattttttaa tgtccataaa aaatcacctt ctgttatagt tccaacatat
    21841 ttaccatcct tacttattat tggaattgca gagtatttgt gatattccat tttttctagg
    21901 gcttgcctta tagtgtagtc ttcatatata tatgctactt cactttttgg tgttagaaag
    21961 aataatatgt tcattacact acccaccgtt actgaaaact tataaatatt actcaatatt
    22021 tataatgtta cttttttgca gtaactttct cctttcttat tttctaagta ttcttaatac
    22081 tatacgctgt aacaagcgtt tttcctttgc tcattttaat cttattatgc tttattaata
    22141 tttttatatg gtctatcagt atcacttatt ctctttttag aaaatttact aaaaattaat
    22201 tgacttaaac ttagccataa taattttact ctacactatt aattttaaca tatttatctc
    22261 attattaatg attttatttt aataatttta atttattttg ctattaattt tatttacaaa
    22321 aactgaaaaa accgctaaat gattagcggc ttatctctca tattactaga acactaatat
    22381 atatgaattt ttaattataa tctaactacg ttagcagctt gaggaccttt agctccatct
    22441 actacttcga actcaacgct ttgtccttct tctaatgttt tgtatccatc tccagttata
    22501 gctgagaaat gtacgaaaac atctccttca ccttctacag atatgaatcc aaatcctttt
    22561 tcgttgttaa accatttaac tacaccagtt ttcattaata aatcctccta aaaaaacaca
    22621 aatatttagc tactcttttc tgtaacctta ttactactct atcatatata gctatgtttg
    22681 tcaattatta ttatttttat acattaataa tcaatttttt tactttataa ttttaaatga
    22741 ttattatctt ttacacttta tttttcttta taatttttta ataatttatt tttattatat
    22801 ggaatcttat ggacttatca ctccagccta aatcccatgg caccaacatt ctatcagtat
    22861 tatggcaagt taccaaagga taccctcttg agtctaatcc tgtcactgtc gaaacatgtg
    22921 ttattctgcc tttcttctca taaccaacaa aatcccctgg acgtaaatta taagcttctt
    22981 tgtatacttc ttcataggaa cctttagcaa ttaatgaacc tcttccacta tacagtgcat
    23041 aatttttaaa agcttgtgca tttacccatg cttttgtacc tgctctctcg ctataattcc
    23101 aaattgagtt ctttttgaac cttccacttt caaacattat ctgtgatgca aagtttgcac
    23161 aatctccacc ttctggatta taatccctat agtttggatt aaatttcata ccatgttctt
    23221 catcagctgc ggctccacag tatttatgtg cataatctat agcctttttt tgttcatctg
    23281 taagctgtaa ctctacacca tcttgttgac caatatagtt tcttatatca tcagatttta
    23341 tattttctaa atttaaagag tctgcaaaag ggtctgtata ccactcttta gttatgacat
    23401 attgattatc tataattttt acatttagat aatgatatgt acctattcta aaggcatttt
    23461 ctttgtcctt ttgattttca tatgaatact tatattcagt cgatactgaa cagattatac
    23521 catagaggtc tttttctttt tcttttattt ttttaatctt aacccttgtc tttatgtctt
    23581 taaattttac accttgctta ctagaccagt tttctaaata cttcatcttt tttacttcat
    23641 tttcataagc ccacagacca aattttttat ccgtctcata aagacctttt aaaatgtttt
    23701 catttttctc taatattgcc ttatttctgt agtcaaataa gttttcaagc aatagtttat
    23761 attggtcttt tacatcatca ccttcaacat ttgcagattc catgactttt tcttccatat
    23821 ttttcatact taaatcattg attaaaaata cacctgctgt agtagctaaa gacaaaaata
    23881 caaatgccat tatacctttg atgtacttat tcttattttt cagtattttc aaagctacac
    23941 ctcccaaata gtacacctta caaatcaaat tacaaagaat attctgtcac atatagtaat
    24001 tttcatattt aataattctc tattttatta tgtccaatat taaaaattaa ttgt
//
LOCUS       DACXMZ010000038        23876 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.038, whole genome shotgun sequence.
ACCESSION   DACXMZ010000038 DACXMZ010000000
VERSION     DACXMZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.038"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            63..1142
                     /gene="mltG"
                     /locus_tag="JD016_05970"
     CDS             63..1142
                     /gene="mltG"
                     /locus_tag="JD016_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAU4999181.1"
                     /translation="MNFKENRLKIAVLIIVILIILTGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            1314..1988
                     /locus_tag="JD016_05975"
     CDS             1314..1988
                     /locus_tag="JD016_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAU4999182.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            1975..3222
                     /locus_tag="JD016_05980"
     CDS             1975..3222
                     /locus_tag="JD016_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAU4999183.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            3294..4964
                     /locus_tag="JD016_05985"
     CDS             3294..4964
                     /locus_tag="JD016_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAU4999184.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDIDKSMKKELEKNGIMVEEKTMRYAKDGL
                     LSHTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKT
                     VDNKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQND
                     ISKSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNT
                     NEILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGL
                     DEEKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNM
                     NTIKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLN
                     KKAIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(5061..5702)
                     /gene="sigK"
                     /locus_tag="JD016_05990"
     CDS             complement(5061..5702)
                     /gene="sigK"
                     /locus_tag="JD016_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009109550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HAU4999185.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTP
                     REREIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            5995..6211
                     /locus_tag="JD016_05995"
                     /pseudo
     CDS             5995..6211
                     /locus_tag="JD016_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6257..6631
                     /locus_tag="JD016_06000"
     CDS             6257..6631
                     /locus_tag="JD016_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999186.1"
                     /translation="MFLLLKIRKRGFISIECIISIAILYVAVYLVSTSLYNCYSFISR
                     NISDREMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCY
                     EINIEIKNEFKKLRFNSYVTRK"
     gene            6615..7331
                     /locus_tag="JD016_06005"
     CDS             6615..7331
                     /locus_tag="JD016_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999187.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWTGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEAVEFTEEDEEYEEELKQNIIE
                     NIKKNFDEKNLVQVYKYRGV"
     gene            7351..7770
                     /locus_tag="JD016_06010"
     CDS             7351..7770
                     /locus_tag="JD016_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999188.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            7760..8284
                     /locus_tag="JD016_06015"
     CDS             7760..8284
                     /locus_tag="JD016_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999189.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIIGNSMGIMNISNYEETFKKTTSIKCRYVDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKMKVWNFSESI"
     gene            8596..9153
                     /gene="efp"
                     /locus_tag="JD016_06020"
     CDS             8596..9153
                     /gene="efp"
                     /locus_tag="JD016_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAU4999190.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            9245..9700
                     /locus_tag="JD016_06025"
     CDS             9245..9700
                     /locus_tag="JD016_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAU4999191.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            9863..10573
                     /locus_tag="JD016_06030"
     CDS             9863..10573
                     /locus_tag="JD016_06030"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAU4999192.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            10627..11697
                     /locus_tag="JD016_06035"
     CDS             10627..11697
                     /locus_tag="JD016_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAU4999193.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNINQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            11725..15279
                     /gene="smc"
                     /locus_tag="JD016_06040"
     CDS             11725..15279
                     /gene="smc"
                     /locus_tag="JD016_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAU4999194.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLESSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     NISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            15306..16586
                     /gene="ftsY"
                     /locus_tag="JD016_06045"
     CDS             15306..16586
                     /gene="ftsY"
                     /locus_tag="JD016_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAU4999195.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            16689..17057
                     /locus_tag="JD016_06050"
     CDS             16689..17057
                     /locus_tag="JD016_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAU4999196.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            17054..18406
                     /locus_tag="JD016_06055"
     CDS             17054..18406
                     /locus_tag="JD016_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAU4999197.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            18444..18716
                     /gene="rpsP"
                     /locus_tag="JD016_06060"
     CDS             18444..18716
                     /gene="rpsP"
                     /locus_tag="JD016_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAU4999198.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            18781..19008
                     /locus_tag="JD016_06065"
     CDS             18781..19008
                     /locus_tag="JD016_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAU4999199.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIILKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            19116..19631
                     /locus_tag="JD016_06070"
     CDS             19116..19631
                     /locus_tag="JD016_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAU4999200.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            19640..20335
                     /gene="trmD"
                     /locus_tag="JD016_06075"
     CDS             19640..20335
                     /gene="trmD"
                     /locus_tag="JD016_06075"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAU4999201.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            20520..20870
                     /locus_tag="JD016_06080"
     CDS             20520..20870
                     /locus_tag="JD016_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAU4999202.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            21218..22087
                     /gene="ylqF"
                     /locus_tag="JD016_06085"
     CDS             21218..22087
                     /gene="ylqF"
                     /locus_tag="JD016_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAU4999203.1"
                     /translation="MNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELLDARIPFSSK
                     NPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIKGVGINKIIE
                     ECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQTGDKPGVTK
                     GKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETLALRLIEKLM
                     KIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTVLNEFRDGKI
                     GNITLEVPENVKR"
     gene            22476..23768
                     /gene="brnQ"
                     /locus_tag="JD016_06090"
     CDS             22476..23768
                     /gene="brnQ"
                     /locus_tag="JD016_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAU4999204.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT        10058 a         2316 c         4370 g         7132 t
ORIGIN      
        1 aattaaagta tctagtttta taatagaaaa tggataaata tacataagga gtagattata
       61 ttatgaactt taaagaaaat agattgaaaa tagcagtatt aataattgtt attttgatta
      121 tcttaacagg tatatttgtg tttatacaaa taggtcctta tgataaaaat aacaaaaaag
      181 atgtaattat cgacgttcca agtggagcat ctgttggaaa aatatctgac atattatatg
      241 aaaataaatt aataaagaat gaactattat ttaaactttt ggtaaaggtt agcaataaag
      301 ctccgtctat aaaatcagga acatacttac ttaatcaatc gtattcaaac aatgatataa
      361 taagtctttt agtttcaggt aaaatatatc aggatggtat aaaagtgact attccagagg
      421 gagctacatc taaagaaatt atagcaatgt tagtaagtaa aaatttagga gataaagcta
      481 cttttgaaaa tttaataaaa aaacctcaag aattttatga taaatttcca tatttgaagg
      541 aagatggaat aacctcttta gaagggttct tgtatccaga aacttattat tttaattcaa
      601 agaaacaatc agaagaagat attttaagcg aaatgttaaa agtatttgac agtaaataca
      661 cggacaagtt taaaaagaaa caaaaagaat taaatatgac tcttcaagaa gtaatggaaa
      721 tggcatcaat aattgagaaa gaagctgttc ttgacaaaga cagacctata atagcaagtg
      781 tgttttataa tagacttaag gtaggtatgc ctcttcaatc agatgctaca atacaatata
      841 tatttgaaga gagaaagaaa attgtaacat atgatgattt aaaaattgat tcaccatata
      901 atagttataa aaataaggga ttacctccta caccaatatc aaatcctggt ataaaatcta
      961 ttgaagcagc attatatcca gagaaaacag actatttata ttttgtagct aagattgacg
     1021 gtggaaataa ttatagtaca aactatcaag accatttaaa atacgttaaa gaatataaag
     1081 aagcaaggga taaacaatca aaagatacaa aagcaacaaa taaagaaaat acaaaaaaat
     1141 aaacaatgat tataaaaagt ctaacagggt aagaataatg ttgggctttt ttattgaaaa
     1201 ttgttattct tagatttata ataaagaaag tgtaaatata tataacaata tgaatttaaa
     1261 aaataataaa ttaatataaa acttgaatat ttattatttt agggagtgtt tttatgagta
     1321 atatagttaa tgatttggtt gaagattata taagagtgac attaaaagaa aaagaaggat
     1381 ttttaaaaga tttagaaatt tatgcagagg aaaatagtgt tcctataata cataaagaag
     1441 tttcagattt attaaaagtc ttgttaaagg ttcaaaaacc aaagagaatt ttagaagtag
     1501 gatgtgcaat tggttattct tctatttttt ttgcaactat tataggaaaa gatgcagata
     1561 taataactgt tgagagaaat gaaaaaatga tagaaaaagc aaaagaaaac ataaaattag
     1621 ctggatttga caataacata actatactag aaggagatgc agaagaaaaa cttagtcaag
     1681 tccaaggtga atttgacctt atatttatag atgcagccaa aggtcaatat aagctatttt
     1741 ttgatttagt tatagataaa ttaaaagatg ggggactttt agtatctgat aatattttgt
     1801 ataaaggtat ggttgctcat gatgattttg ttgtaagaag aaaaaagact atagttaaaa
     1861 gaatgagaaa ttatttagat tatatatgta attgtgacta tttaagcaca tcattggtac
     1921 caataggtga tggtgtagca ctaagttata aagaaagtaa aagaggtgat gtagatggaa
     1981 ttaaataaag tagaactttt agctccagca ggtgatttgg agagattgaa aatagctata
     2041 acatatggag cagatgccgt ttatataggt ggagaaattt ttggaatgag gtctgcagcg
     2101 aagaatttta gtaaggaaga tatggcagag ggagtagctt ttgcacatga aagaggcaag
     2161 aaagtattcg taacagtaaa tataatacct cataatgaag attttctaca attagaagac
     2221 tatttgttgg aactagaaga gataggaata gatgcagtta taatagctga cccaggagtg
     2281 cttagtgtca ttaaaaaagt aattccgaat atggaaatac atttaagtac acaagcaaat
     2341 acaaccaatt atctaagtgc aaacttctgg tacgagcatg gaataaaaag agttgtagtg
     2401 gcaagagaat tatcttttga tgaaatatct gagataagag caaaaacacc tttagatatg
     2461 gatatagaag cgtttatgca tggtgctatg tgtatttctt attctggcag atgtttaata
     2521 agcaactata tgactggaag agatgcaaac aaaggttctt gtgctcaatc ttgtagatgg
     2581 caatatcatt tggtggaaga aaaaagacct ggtgagtatt ttccaatata cgaagatgaa
     2641 aggggtacat tcttctttaa ctcaaaagat ttatgtatga tagagtatat accagagtta
     2701 atcaaatctg gtataacaag tttaaaaata gaaggtagaa tgaagactgc atactatgta
     2761 gctactgttg taagagcata cagaatggca atagatgaat tttacagaga cccagaaaac
     2821 tggaagttca atccaatgtg gatggaagaa ctaaaaaaag gtagtcatag acattttaca
     2881 tcaggttttt acttgaataa accaacaaca gaagaccaaa attatcaatc agcatcatat
     2941 gttagaaact atgattttat aggtatagtt agagaaacag aagatgaaga tggtcttatt
     3001 gttgttgagc aaagaaataa aatgtgtgtt ggtgatgaaa tagaagttat gggaccatat
     3061 aaagaaacta tgtttactaa aattgaagaa atgtacaatg aagaaggaga agctatagaa
     3121 tcagctcctc atcctagaca aatagtgaag ttaaagctat cagtaaaagt tggtaaagat
     3181 tatatgctta gaaaagttat agaagaaaag gttgaagaat aataaaaaat tgataaaaat
     3241 agtattagcc ccttagaaat tgtcaataat gaaattatct aaggggtgat ttcatgtcaa
     3301 aaaagaagac tccatttttg aaaaaagtgg gaaagagaag ctggtgtata tttactataa
     3361 ttttaataat ttatagtgtt ttaatttata ggctagttga tatacaagta ttaaaaggtg
     3421 ataaatacaa acaaagtgtt gaatcacaaa gcgttgagaa agtcgagtta aatagtggaa
     3481 gaggaataat ctatgataga aacaacaaga agcttacaga tacaagtaaa tctcaggtat
     3541 taattgttga aaaagaaaaa ttaaataata actataaaat tttggagctt attaagaaag
     3601 ctactaagat gaatgattta gatatatata aagctgtaca agagcaattg acacgcccta
     3661 taatacagat tcagactaaa aatatcgata tcgataagag tatgaaaaaa gaacttgaga
     3721 aaaatggaat aatggtagaa gaaaaaacta tgaggtacgc aaaagatggt ttattatcac
     3781 atacaatagg atatataaaa gaagatgata aatcaggtca atcaggaatt gaaaagagta
     3841 tggatagtgt acttagaaat tcaaatgaaa agtatataag tgcttttaaa gctggagatg
     3901 caggaaatga aaaatctcta aacatactaa agggaagtgt aaaaactgtt gataataagg
     3961 ataaagatag acatctaaaa actactattg attataacat tcaaaaaaaa ttagaacaaa
     4021 tactaaataa agaagaaaat cctacagcag caataatctc ggaagcatct acaggtgaaa
     4081 ttcttgcaat gtgctctaga cctaattttg accaaaatga catatcaaaa agtttaaaag
     4141 gaaaaaatgg agaatttgaa aatagagtta taaaagcgac atatccacca gggtctgtat
     4201 ttaagatggt agttttattt tctgcacttg aaaatggtgt tatagatgag aactatactt
     4261 acaactgtac aggtaagact aaagttggga atactaatga aatactgcga tgtaataaaa
     4321 gagatggtca tggtttccaa aacttaagac aagctttttc taattcatgc aatcctgcat
     4381 ttttagatat agctatgaaa cttggaaaag aaaagatact caaatctgct gaaaaattac
     4441 atttatttga aaaggttgat attggtcttg atgaagaaaa aattagagaa gctccaaaaa
     4501 acatatcaat aagaaattta gctataggtc aagaaaatat agaatttaca ccacttcaaa
     4561 taaatcaaat gactcaaata atagctaata atggaacatt caaaccacta tacttatata
     4621 aaagtcttgt ggacaataat atgaatacaa taaaaactta taagtcttca aaaaaagaag
     4681 aacttatttc tccttatgtg tgtacacaag ttaaagaata tatgaagtct gtatctagga
     4741 ctggtacagc taaagaccta aaagatattg aaggtggttg tggagttaaa actggtacag
     4801 ctcaaagtag tttaaataaa aaagctatag accacggatg gataacagga ttttatcctg
     4861 aagaaagacc taagtatgta ataactgtgt tagtagaagg aactcaaaaa ggaaataaat
     4921 ctgcaacacc tatatttaag gaaatatgtg aaagtataaa ataaattatg tactataaaa
     4981 tttaaggata tatcgtaact ttgattttag atagaaaatt ttgtctaatg atgtacgata
     5041 tatcctttta aaatatagta ttaactttct tgaacaagct ctttttctag ttttttaaga
     5101 gctttttttt cgattctaga tacatatgac cttgatatat cgagtttttg tgcaatttct
     5161 ctttgtgtct tatagccata aggagttaac ccatatctta actggacaat ttctctttct
     5221 ctaggagtaa gaattttgtt tagttgttcg taaagttttc caacttgaac ttttagttca
     5281 acttcatcaa gaacatagtt agcctctgta ccaagtatat ctaaaagact tatttcatta
     5341 ccttctttat ctgttccgat agggtcttgt agagatactt gagttttatt ttttttgtta
     5401 gatctaatat tcattaaaat ctcattttca atacatctag aagcatatgt tgctaatcga
     5461 gttcctttat ctatgttata agtttctata gctttgatta atcctattgt acctatggat
     5521 attaaatcat cctgatcttc cgttgaatta ttatatttct ttgctatata agctactaat
     5581 ctcatgtttc tttcaattaa tgtatctttt gcacttttat cattttctat tttaaattta
     5641 gttaaatact caatctcttc ttcaggggtt aagggttttt caaaagattt aagagctgcc
     5701 aataggtcac ccccttttca acggtcactt taaattatat gaaggagact tatccaagtt
     5761 gactgttatc taataaaata atatgcatat ttttagaatt taaaacatat atttgcaaac
     5821 ttttgaatgg aatatggcat aaagagtggg aatttaaatt taagtttttg ttgtatatat
     5881 atgaaacaag gcgaagaaat tattgattta taaagtataa attggaattg aggtgtcaat
     5941 aaatataaaa ggaaatagta tttatatatg ttttggtgtt gaagtaaaag attaatgcaa
     6001 agcattagag tctataaaaa tatgaatata ggaggtgtaa ttttacaata cttaaaaaag
     6061 taaatgtaat taattatttg tataaagttg attacaagca aaagcatcag tcatagttaa
     6121 tactgttaag gatgaaggag gtgtgttaat ttatatatgt atgggaagaa attttgatta
     6181 caataaaaaa acttacaaaa gatatttatg aaaaaactta tagttaaatg tttatatata
     6241 aaatattatt taggggttgt ttttattatt gaaaataaga aagagaggtt ttatatcaat
     6301 cgaatgtata ataagtattg ctatattata tgtggctgtt tatttagttt ctacatcatt
     6361 gtataattgt tatagtttta tcagtagaaa tatatctgac agagaaatgt taagtacagc
     6421 aaaaaaatat atagaagatg agaagtatag aatacaaaat agtaagtatg agttaattga
     6481 agataagata gaaaaaaatt acataaatgg atatgaaatt aacagtagaa tagagcaaat
     6541 tttagattat tatcaatgct atgaaataaa tatagagata aaaaatgaat ttaaaaaact
     6601 gaggtttaat agctatgtta ctagaaaata aaggctctat attaataata acattgataa
     6661 tattttctat tataagtact gtgtgcatta tgtgtatagg tttaatttat acacataata
     6721 atatgtttga gttaggatac aaagacattc aactaaaaga aatttcttta agtggaatag
     6781 aaatatcagt aagtaatgta ctttcttgtg ttgaggaagc tattgatgta tctaataatg
     6841 aagacgaatt tagagaatat ttcttaggaa ataatatgat tgaatgtata aaaaaaataa
     6901 aagatacatc ttatagtagt ttagaagaac tttctttaaa agtagaaaat aatactattt
     6961 atgacagagg agattttttt gaatttgata tagaaagtaa tgttagagaa aaagaatttt
     7021 taaaagcaat aaaagtaaga gtgaaaatca aaaatccatg gacaggaatt gatattgaag
     7081 attatatgga taaatctaat aacaataaaa ttgatgacaa aacattcaat aattataaag
     7141 catctgaaaa taatgaaatt ctaaatcaat ttaaaaatga aagtgaaaat ataaggaatc
     7201 aagaggctgt tgaatttaca gaagaagatg aagaatatga agaagaactt aaacaaaaca
     7261 taattgaaaa tataaagaag aattttgatg aaaaaaacct agtacaggta tataagtata
     7321 ggggtgtata actttgaaaa aagaaggaat gtgactgtaa tagaattaat agtatctatc
     7381 agtataatac tcttgatagt tacattatct tttccaaaag ataatcttga aaatcataaa
     7441 ataaatttat ttgcacgtca actttgttca gatataaggt atgttaggcg aattaatatg
     7501 tttagcaact acaatacata tatatattat ataaatcaag gtaaacacga aggttatgta
     7561 ttgagaaaag atggtaaaaa cattaaatca ataatgctac ctaaaaatgc aaaaatagct
     7621 aggagtattg aaataataaa gtttaaatct gatgggtcgc ctcaaaaggc gtctacaata
     7681 gagatatata ataatagatt aagaaaaact ataacaataa cacctgtaag tggaagagta
     7741 cttctgaaag agggaaaata tgaaacataa atatggttac ttgcttttgg aaagtgtagt
     7801 atcattgtct tcaatggtga taattatact agttttatat tctatatttt tgtctacaat
     7861 aaacttaaag ttaaaagtag aagataagat agaactacaa caacaaagtc ttgaaatcat
     7921 aaaatctatg gaaggaatta ttggcaattc tatgggaata atgaatatat ctaattatga
     7981 agaaacattt aaaaagacaa cgtctataaa atgtagatat gtagatgaaa ataataatga
     8041 agaaggcata agtaataaag aaattatatt gaatgaaaga cgaaataagt tatttgtaaa
     8101 ttctttaaat ggcgaaagta gtcaagcagg tggttatgaa attggagatt atgtagatga
     8161 aatgtatgta tcaattacta ataatggtca atacgttaat attaaattga aattatcaaa
     8221 acgtagtcaa aaatatgaga ctgatttcaa aatgaaagta tggaatttta gtgaaagtat
     8281 ataaaaatac ataaatttaa gagaaagaaa tgacatcaat aagtcatata aaatataatt
     8341 attgagaact atataactaa ataatatcaa tatgtaaagt gtatagatta agaatataga
     8401 ggataataat aaaaatatag aataagtaat ttgccaaata cttgtttata ttgtataact
     8461 tatgatatta tgatattagt aaaaatttgt aatcctgact tgacagaatt caagtaagag
     8521 atataatttt aaaggcatag taatgtaaaa tttactaaaa aataagaaat ttattttaat
     8581 atggaggaac aaaaaatggt atcagcaggt gattttagaa aaggtgttac atttgaaaaa
     8641 gatggacaac catgtttagt agttgatttt caacacgtta agccaggtaa aggagctgct
     8701 ttcgttagaa ctaaatacaa aaacttaaaa acaggagcta taagagaaga aagtttcaat
     8761 ccaagtgaaa aattccctaa agctgttata gatacaagac aaatgcaata tctatacaat
     8821 gatggtgagt tatattattt tatggatcaa gaaaattttg aacaaatacc attaaactat
     8881 gagcaagttg aagatgcaat taagttctta aaagaaaatg aagttgctac aataagattt
     8941 taccaaggac aaccattcca agtggaagcg ccaaactttg cagagctaga agttacagat
     9001 acagagccag gtataaaagg tgatacagca agtaatgtaa ctaaagcagc tacagttgaa
     9061 actggagcag ttgttcaagt tccattattt ataaatactg gagataaagt aaagatagat
     9121 actagaacag gtgaatattt atcaagagta taaaaattta tgtatataaa agcttatatg
     9181 tcttaataat ataagatata tgagctttta gtttatatat aaaaaagaaa gcgaggggtg
     9241 ttacatgaaa catttatatg aagaagttgc atacttaaaa ggcctagcag aaggactaga
     9301 aataagtgct gaaagtaaag aagggaaaat gatacataaa atagttgatg cattagaagt
     9361 atttgcagat gctattgtaa cattggatga agaacaagaa gaacttcaag attttgttga
     9421 atctatagat gaagatttag cagatttaga agaagatatt gctgacatgg aagaagatct
     9481 ttatgaagaa gatgacgatg aggatgatga ggacttcagc tatatagaaa tggaatgccc
     9541 aaattgtgga gagttagtag aaatagacga agatttgtta tatgatgatg aagtagatgt
     9601 tgtttgtcct gattgtaagg ctgtgatatt atcatcagaa gatgattgtg atgatgattg
     9661 tacatgtggt ggttgttcaa gttgtgatga tagagagtaa aaattcataa gaattaaaac
     9721 tgcctatgat gcctatgaga tattttgctt cataggtagt tttattttta tgaggaaggg
     9781 tattatgtat tttggttttg caatacttgg acaaaaatag tacactagaa tttatagaat
     9841 aggattttgg agggatgtaa atatgaaaat aagtaaaaaa ttactagata atatacaaag
     9901 atttgaaaat gttataaatt ataaatttaa aaataaagaa tatatactag aagctcttac
     9961 tcatagttca tattctaatg aaaataaaaa atataatttt aacgaaagat tggaattttt
    10021 aggggattcg gttttaggca tagtgataag tgattattta tttaatgaag aagctaatct
    10081 gcccgaaggg gaactaacta aacttagagc aaatatagtt tgtgaagatt cattaagtga
    10141 agtagctaat gatataaatt taggtattca catgttatta ggtagaggag aagaagcaac
    10201 aggaggaaga cataggactt caatattagc agacgcattt gaagctgtaa tagctgctat
    10261 atatcttgat ggaggttttg aaagtgcaag acagtttata cttcatcata tggaaaatat
    10321 aatttatgat tcaagaaaag gaaatatatt tagagactac aaaacgcatc ttcaagaggt
    10381 gttacaaggg aatggagaaa ataatatctg gtacaggctt atagaagaaa aaggaccaga
    10441 ccataataaa agatttgtaa tggaagttgg aattaatgac gatgtacttg gtataggaga
    10501 aggcaagagt aaaaaagaag ctgaacagtt agctgctaaa atagctttaa agaaaaaatt
    10561 atgggaaaaa taaatataat atgtaaaaat ataaaaagaa atatttatat tagaaggatg
    10621 ttgtagatga aaaagagaat tatacctata tttgttcctc acaaaggatg tcctcatgat
    10681 tgcatatttt gtaatcaaaa aaaaataaca ggtgtgagta ctgatgttac tagtgaagat
    10741 gcaagaaata taattgagga atgtttagag actatagata aagatgcaga tgtagaaata
    10801 gcattttttg gtggtagctt tactgctata gatattgata tacaaaaaag tttattatca
    10861 gttgctaaag aatatgtaga aaaaggtctt gtaaaagata ttagaatgtc tacaagacca
    10921 gattgtattg atgaagatat attaagtatg ttaaaagaat ataagacaag tataattgaa
    10981 ctaggagtac aatccttaga tgaaaaagta ttattagata gtataagagg tcatcaatct
    11041 gaaattgtat acaagagttc aaagatgata aaaaatagtg gaatcaaact tggattacag
    11101 atgatgattg gtcttccagc tgacacagaa gaaaagtgta tatttacagc aaaaaaattt
    11161 gtagagctaa aaccagattg tgttagagtc tatccaactt tggtagtaaa agatacaggt
    11221 cttgaaaagt taatggaaca aaataaatat aatccattta ctcttgaaga gagtgtaaat
    11281 atagtaaaaa aagttcttgt actgttttat gttaataata tcaatgtaat aagagttggg
    11341 cttcaagcta ctgatgatat acaaattgga aaagctgttt tagcaggacc atatcatcct
    11401 gcatttagag agttggtaga agcagatatg ataaaagatt atttagaatt tgttattttg
    11461 caaaataaaa atataaatca aatgttggtc aaatctaata aaaagaatat ttctaaaatc
    11521 ataggaaata aaaaaacaaa tgttaaatat atggaagaaa aatttggtgt gcttttaaaa
    11581 acacaagaat cagatttaga tataaatcaa ttagaaattg tgttagatgg gaaaagttta
    11641 attagtgcta atatgagaga cattcataga aaattgtatg acatttatga cctttagttt
    11701 taataatgaa gggagagaaa agatttgtat ttaaaaagac tagagttaaa gggatttaaa
    11761 tcctttccag taaaaacaga tattattttt aaagaaggaa taacagccat agtaggacca
    11821 aatgggagtg gaaaaagtaa tatatctgat gctgtaagat gggtattagg agaacagagt
    11881 attaagagtc ttagagggga taaactcgaa gatgtaatat ttgcaggtac agatacaaaa
    11941 aaacctatga attattgtga agtggctctt actattgata attctgaaaa tcaattggaa
    12001 ttagatttta cagaagtaac tataagaaga agggcttata gaaatggtga aagtgaattt
    12061 tttctaaata ataaaagttg tagattaaaa gatataaaag aagtcttttt agatactggt
    12121 attggaaaag atggttattc aatcatagaa caaggaaaag tagatgaaat attaagtaat
    12181 aatcctttaa gcagaagaaa agtttttgat gaagcttgtg gcatatcaaa atatagatat
    12241 aaaaaacagg aagcagaaag gaatttaagt aatacaaagg aaaatttaga aagaatagat
    12301 gatgtatata tagaaattga aaatcaatta aagcctcttt ttaatcaaca gacaaaggca
    12361 aaaaagtact tggaaataag tgaaaaatta aagacacttg aagtaaatag ctttataaga
    12421 gaaattgaag gaatagaaaa agaattaagt gaggttaatg aacatcgtaa ggttattgaa
    12481 aaggagctca atgaaaaaga agaacaaaaa aatgttgtag agaaaaagca agaagatatt
    12541 aataaagagg tagaggtatt acaagatgta attgaaaaat ctgtagatta tataaattca
    12601 attaaaggag ttatatcaaa aaaagaatcc caaataaatt taattaagga aagaataaga
    12661 aattttacga atgaaattag tagaaaaaat ttagaaataa aggatataaa agagaagcta
    12721 aatgaaaata agcaatacat aaaagaatta gaaagcaata aattatcagg tagtgaagaa
    12781 ctatctacct tgcaggaaaa tataaaagta ttggaatcaa gtaaagataa acaaaaaatt
    12841 aagttagaat ccctaaataa tgagatagaa ttattgaaag aaagtataat tgatatatta
    12901 aataagaaac aagagtttag taataagctg tccactttaa acgcaaataa agaaaatatg
    12961 aatatcagag atgaaaatat aaattcagag ataacagagt taaacaaaaa tattgaaata
    13021 aaatcatctg aattagatac tataaataaa gaatttaata tgcaaaatga aaatttaaag
    13081 aatgtaaata atagacataa agaattatca attaatttgc aggattcaat tagtgaacac
    13141 aataaactag aagatgaaat acagaaaagt aaatataatt taaatggata taattcaaag
    13201 ttaaatgtct atatagatat ggaaaatcat tatgaaggat ttaatagagg agtaaaagaa
    13261 gtactaaaaa ataaaaattt aaaaggagtt catggagcat taggacagat tataaatgta
    13321 cctgaaaagt atgagaaatc tatagaagca gctcttggtg cttatatgca aaatataata
    13381 actgataatg agtttagtgc taagtctgca ataaactatc taaaacagaa taatttaggt
    13441 agagtaacat ttcttccatt aaatataatt aaatcaaata aaataagtct aggaaattta
    13501 aaagcaaata ctaagttcat aggaatagca agtgatttaa ttacatttga tgaaaagtat
    13561 agaaatataa ttgaaaatat acttggaaga acaatactta taaataatat agatgagggc
    13621 ataaaatttg caaaagaaac tggtcatagg tttaaaatag taactttaga tggtgaaatc
    13681 ttaaatccag gaggttcttt gactggggga agtctaaaaa ctaatgggaa tatactatcg
    13741 agaaaaagat acattaatga atatacagaa aacataagta atatcaagaa tgaaatttca
    13801 catctagaat taaaaagaga atctttagac aaagatgtga aaaatatcaa aaatgaaatt
    13861 gattcacatg aaagtaaaat aaaagattta gaaaaaagta taataataaa aagtacaagt
    13921 ataaagaatg ttgagtctga aattgaatcg ttaaaaggta gtattactaa attagaaaat
    13981 gagaagaatg atttaaattc taacctaaat tatacacttg aaaaaagtga tgatgttaga
    14041 aaagatatgg aagagttaga tgatttatac aataaaaata aagaaaaaat tgatgcattg
    14101 aatgaggaaa taaaaagata caatgattta tatgataaag aaaaatctga atttgatgaa
    14161 ttgaacttaa gtttagtgaa gaaaacagaa gtttacaata gtatagttag agatataaaa
    14221 agaatatctg gtgaaaattg tgagctagaa gaaaaaaata aacaattaga agagtcatta
    14281 aattatgaag aacatgaaat aattaaatta caagattcta tactcactga agaaaaagaa
    14341 aaagaaaatt taacaaaaca actaggggat agtaatagaa atcttgaaac tagaaaaata
    14401 gcaaaagatg atttaaaaaa tagttttgat gaaattaata aagaattaaa aactatagat
    14461 agacagcata tagaacttaa agaaagttta tttaaagttg gtggaagatt agaaagatta
    14521 aaaactagtc aagacacata tataaataag ttgtttgagc aatatgatat gaccttagta
    14581 caagcattgg aaattaaaga tgaagatttg gatatagata gaaaattctt agaaagtcta
    14641 aagagagaaa taagaagtct tggaaatata aatatagact ctataaagga atatgaggaa
    14701 ataaaagaaa gatacgattt ttatagcgaa caaaagcaag atttagaaga gtctatggaa
    14761 gagatagaaa aacttataca tactttagaa gaaaatatga aatctgaatt tgaaattaag
    14821 tttgaagaaa taagtaaaaa ttttaaatat gtatataaga gattatttgg tggaggttgt
    14881 ggagaattaa ctattttaga taaagaaaat ttattagaaa gtgatatact aataacagct
    14941 caacccccag gtaaaaaaat gaaaaattta aacctattat ctggtggaga gaaagcttta
    15001 acggcaataa gtatattatt tgctatattg attacaaagc caacaccatt ttgtatatta
    15061 gatgagattg aagcgccact tgatgacgca aatatattta gatttggaga atttttgaag
    15121 gatttatcta aagagactca atttatatca gtaactcata ggagaggaac tatggaagct
    15181 gctgattata tctacggagt tacaatgcaa gaaaaagcca tttcaaaagt tattagttta
    15241 aaattaaaag aagctcagga aataacagat attatataac ttagattgac ataggaggaa
    15301 taacagtgtt aaaaaaatta tttggttttg gaaaagacaa ggaaaaggaa atagaaaaaa
    15361 aagatgctga agaagaaata gaagttgaag acagtgttga taatttagaa aacttggaag
    15421 aaactatttt ttcaggactt gaagaagaag tgatagataa agtggaagat gttgaagaaa
    15481 aaaatgagga aagtgtcaat aatgaaggca ctgaagaaat cgaaaacttt gaaaaaatag
    15541 aagttgagat ggatagtaat ggaaaagaag tagaaaatat ttcaaataat gattctcaag
    15601 aagagataat cgaagaatta gaaaattatg atgaaattga gtctaaagag atagaagata
    15661 aagaagataa gaaagttaac ttgtttgaaa gactaaaaca aggattgaca aaagcaaagc
    15721 aaggaataac tgatagaata gatgaagttt taaaatcata tacaaagata gatgaagaat
    15781 tacttgaaga tttagaggaa attttaataa cagcagatgt tggtgtaaat acaactatgg
    15841 atataattga aagactaaga gataaaataa agcaaaaagg tattactgaa ccaataaagg
    15901 taagagaaga acttaaatct atagttgaag atattcttac aaatgaaaac tctacactag
    15961 atatagaacc agcaccatgt ataatattaa tggtgggtgt aaatggggtc ggtaagacta
    16021 ctacaatagg taaactagca aatagataca aaaaagatgg taaaaaagtt ttattagcag
    16081 cagcagatac atttagagca gcagcaacag agcaattaga aatttgggca aatagaacca
    16141 atgtagacat aataaaacat caagagggag cagaccctgg agctgtagta tttgatgcta
    16201 taaaagctgc aaaagctaga aaaacagatg tattaatatg tgatacagca ggaagattac
    16261 acaataaggc taatcttatg aatgaacttg gaaaagtatt taaaatagta gatagagaat
    16321 ttccagaagc aaaacgtgaa gtacttcttg tggtggatgc tacaacaggt caaaatgcag
    16381 ttgttcaagc aaagacattt aaagaagttg cagatataac aggtatagta cttacaaaac
    16441 ttgatggaac tgcaaaaggt ggagtagtcc ttgcagtaaa atcagaagta gatgtacctg
    16501 taaaacttat tggagttgga gaaagtgtag aagacctaca agatttcaat gcaaaatcat
    16561 tttcagatgc attatttggt aactaatgtt cttttaaaaa taatggttga caagagtgtt
    16621 gttatgatat aaaatacaat agtgtaaagt aaataacctt tacagtatac tgctaggaag
    16681 tgtaagatat gaatatagaa aaaatggtcg aaattggatt gttatttgaa cagtataaag
    16741 aattattaac tgacaagcag aaagaaatag ttgctctata ctatgaagaa gattattctt
    16801 taggtgaaat tagtgaaaat ctgaatgtat caagacaagg tgtttatgat accttaaagc
    16861 gttcagaaaa aatactaaga gattatgaag aaaagttaca cttagtatca aagattcaag
    16921 agcaggaaaa aaatattaag attataaaag ataaaattat tgacattaaa gaagatttat
    16981 tgcacaatag agattgtgct aatttaatcc ctaagctaga aaatatagaa gatgtatgta
    17041 gggagatgat aaaatgatat ttgaaggatt gtctgataaa ctacaagggg catttagtaa
    17101 gttaaaatct aaaggaaaac ttacagaagt agatgtaaaa aatgctatga gagaagttaa
    17161 acttgctctt cttgaggcag atgttaactt taaagtagtt aaagatttca taaaaaaagt
    17221 tcaagagaga tgtgttgggc aagaagttat gcaaagctta actccagctc agcatgtaat
    17281 caaaattgta aatgaagagc ttacaagttt aatgggagat gtacaaagta aagtaatgat
    17341 ttcctcaaag cctcctacaa ttttaatgat ggttggattg caaggtgcag gtaagactac
    17401 tacagcagga aaacttggag gatattttaa aaagcaaggt aaaaaacctc tattagtagc
    17461 ttgtgatatt tatagaccag cagctataaa gcaacttcaa gttgttggag aaaaattgga
    17521 tatacctgta ttcaatatgg gagataaaga aaatccagta aatattgcga aagcaggttt
    17581 aagccatgcg attaaaaatg ctaatgattt agttattata gatacagctg gtagacttca
    17641 tatagatgaa gtacttatgg atgagttaaa gtctattaaa tcagaagtta agccacatga
    17701 gatacttttg gttgtagatt ctatgacagg tcaagatgct gtaaatgttg cagaaagctt
    17761 caatgaggca cttggtgttg atggagtagt attaactaaa ttggatggag atactagagg
    17821 tggtgctgca ctttcaataa gagcagttac acaaaagcct ataaaattta tggggatggg
    17881 agaaaagcta gatgatatag aacctttcca cccagataga atggcatcaa gaatattagg
    17941 tatgggagat gtcttaagtt taatagaaaa agcacaagaa aatatagact tagagaaagc
    18001 taaagaatta gagcaaaaaa ttaaaaaaca agagcttgat tttgaagatt tcttacaaca
    18061 aatggaacaa atacaaaata tgggacctct tgacaagata cttggtatga tacctggtat
    18121 gggaaatgta aaagaccaac ttggagatgt agatttgaat ggcaaagaaa tgaagagaac
    18181 aaaagccata attcagtcaa tgactacata tgagagaaga aacccaggag tattaaatgc
    18241 ttcaagaaag aaaagaatag ctaaaggcag tggaactagt gtacaagatg taaatagact
    18301 tataaaacaa cttaatgaaa tgaaaaaaat gatgaagatg tttgctggtt cacaaaagag
    18361 tatgaagaaa aggggcggct taggcggttt accttttttt aaataaaata taaaaaagta
    18421 aaaattattc ggaggtgact gtaatggcag ttaaaataag attaaaaaga atgggagcta
    18481 acaaaaaacc tttctacaga atagtagtag ctgattcaag agctccaaga gatggaaaat
    18541 ttatagaaga aataggatac tataatccaa tttctgaacc taaacaagta agaataaatg
    18601 atgaaaaagc tataaaatgg ttagctactg gtgctcagcc aacagaagta gttaaaaaat
    18661 tacttgtaaa aaacggagta atagaaaaat tcgaagcttc taaacaagca aaataatttt
    18721 ttcttggcaa ttagtttaag taagggctca ttgctaaggt tactttagga gatgaaatat
    18781 atgaaagagc tagttgtaga tatagctaag gctctagttg ataatcctga ctcagttgtt
    18841 gttgaggaat ttgaagataa tgatggtatc atcttaaagc ttacagttgc tcaagaggat
    18901 atgggtaagg ttattgggaa gcagggaaga atagctaaag ctataagaac tgtcgtaaga
    18961 tctgttgcaa atagagaaaa tataaaagtt tctcttgaga tagtataaaa taggaggatt
    19021 aggtataatg cctagtcctt tttatataaa tgcgtagttt aagattttat gatgatatga
    19081 ccacataaga ttaaattttt taagaaggtg ataacatgaa agaaaaatta actcatttta
    19141 aggtaggtca aatagtaaat acacaaggtt taaaaggtga agtaagagtt tatccgctta
    19201 ctgatgatat agagaggttt gatgaattaa aagatttcta tctgggaaaa gatttaaata
    19261 ataaattggc tgttgaaaaa gtaagataca aaggttctat ggttataatg aagataaaag
    19321 gcatagacag catagagaaa gcagaaaaac taaaaaataa atttatgtat gtatctagag
    19381 aagatagcag agagcttgaa gaaggcgaat tttttatagc agatatgatt ggaatggaag
    19441 ttttaactgt agatggaaaa tatgtaggga tactagaaga tgttcttcaa tactcagcaa
    19501 atgatgtcta tgtagtaaaa ggtgaggaag ataaagaatt tatgatacca gctataaaaa
    19561 aatttgtacc tacaatagat attgatgaaa gaaaaatgat tatagaccct attaagggaa
    19621 tgatagatta ggtggtaata tgagatttca tataatgaca ctttttccag aaatatttaa
    19681 ctcgtacatg gatgaaagta taatgaagag agcagttgaa aaaggtataa ttgaggttca
    19741 tatatataat ataagggatt tttctaataa taaacataaa aaagtagatg attatccatt
    19801 tggaggagga gcggggatgg taatgactcc tcaacctata tatgacacat ataagcatat
    19861 aataacaact cataatataa ataacccaag tgtaatttat cttactccaa aaggtaaggt
    19921 atacaatcaa tctatggcaa agcagatgtc tttaaaagaa gatataatac tactatgtgg
    19981 tcattatgaa ggaatagatg agagaatcat tgatttaata gttactgatg aaatttcaat
    20041 tggagattat gtattaactg ggggagagct cccagcactt ataatgatag attctatatc
    20101 gagattaata ccaggtgtat taaaccaaga agaatcattt gaagaagaat catttaaaga
    20161 caacttattg gaatatcctc attacactag acctagagat tttgaaggat taaaagtacc
    20221 agaagtgctt ttatcaggga atcataagaa gatagatgag tggagaagag aagaatctat
    20281 aaggataacc aaagaaagac gatttgattt atataaaaag tctaatgaaa aataaaaata
    20341 taatgaaaaa ttaatatata tgtatacaat aaagtaccta taaatggaat taaaaacata
    20401 aaagtgcttg taataactat tgaagtatag tataatataa aatgttgaaa atacgggcgt
    20461 tcctctgttt acactggtcg ggtagttaag aacgtctatg atatctagga ggaagaaaga
    20521 tgaatgaaat gctaagagca attgagcaag aacaattaaa aaatgaagtt cctaattttg
    20581 gacctgggga cacagttaaa gtacacgtta gaattataga aggaaaaaga gaaagaatac
    20641 aagtattcga aggtgtagta ttaaagagac aaggtggagg agcaagagaa actttcacag
    20701 ttagaaaaat gtctttcaat gttggtgtag aaagaacttt cccagttcac tctccaaaaa
    20761 tagaaaaaat agaagttact agaaaaggta aagtaagaag agctaaacta aactacttaa
    20821 gaggtagagt aggtaaagct gctaagataa aagaagctag aaataaataa ttaaaaactt
    20881 tacttataaa tggggggctg atttatcagt cctctttcta ttttaatgaa aattacagca
    20941 aataattata ctagaaataa atagaaatat ttgttgaaaa tatacattat ttatctatga
    21001 atttatagct tttaaaatga aaaataaaat tggtaaatat tgcattatat tttgtaacaa
    21061 tattaaaaat aagagaacag tattatgtaa agtatgacgt taaaaagatg acaatattat
    21121 agtttgttaa gatagatttt ttaagtggaa ttaggatata ataatataat attaataagg
    21181 agtgatacat gtgtcaaatg aatacgaaga gtatcttatg aatgacaatc tacatataaa
    21241 ctggtatcca ggtcatatga aaaaaactaa ggagttagta aaaaataact taaaattgat
    21301 tgatgtagta attgaacttt tggatgctag aatcccgttt agtagtaaaa atccagacat
    21361 agacaggctt gtaggagata agccaagagt tgtcgtactt aataagagtg atatggcaga
    21421 tagggataaa ttgaatcaat ggattgaata ctataagaaa attaatatca aagctatacc
    21481 agtagataca ataaaaggcg ttggtataaa taagataatc gaagaatgta aaaatgtaac
    21541 tagagaaaaa atgagttctt taaaagacaa agggagaaaa gaaagagcta tacgaataat
    21601 gatagttgga gttccaaacg ttggtaaatc gtctcttata aacaaattaa caggtagaaa
    21661 aagcactcaa actggagata agccaggagt aacgaaaggg aaacaatggg ttagattaaa
    21721 gggaaatctt gaacttttag atacacctgg aatactttgg cctaagtttg aagaccaaga
    21781 agttgcatta aatttagcat ttagtagagc tataaaggat gagattttag atactgaaac
    21841 tttggcttta agacttatag agaaacttat gaaaatagaa cctgagaagt tgaaagccag
    21901 atataagtta gattgtctag gagaaactcc aattgaaact atggatatga ttggccataa
    21961 aagagggttt ataacaggta aaaaagaact agattataca cgcatagcca caactgtatt
    22021 aaatgaattt agagatggaa aaataggaaa tataacattg gaagttccag aaaacgttaa
    22081 aagatagatt ttaaaacaaa atcaaaaatt taatgttaaa aatttaaaaa agccttttag
    22141 caaaggcttt tttatttttt tgaaatatag acaaatatct ccatataaaa gtataaatta
    22201 ggttagaaaa cctttaaaaa tattaattta agacaaatat acctaatatt ttgaaaatta
    22261 aaatgagtgt gattatttaa aattttagca tattgacttg cttgtttcgt ataatatata
    22321 ataagcacaa ataaatacaa tatattaaaa atttaattta tttaatttta ttaatttaat
    22381 aataagttct tattgtttaa ttcaacttac ttatgcaagc gtaaacaatc gagacatttt
    22441 attaatttaa taaggttaaa aagggggatt ttataatggg taagaccaag gatgttatag
    22501 tatttggatt tgcgttattt gcaatgttct ttggagcagg taacttaata tttccacctt
    22561 acttgggaat aattacaggt ccagaatggt taattgcctt cttaggattt acgtttgctg
    22621 atgctggatt ggcacttcta gcagtaatgg ctacagctaa attcgatgga aacgtagttg
    22681 aaatgtttaa aagatgtggt atgaagttag ggattcttat aggttgtgca gatattttat
    22741 gtataggccc attcttagct ataccaagaa caggtgcaac aacttatgaa atgggtataa
    22801 tgcctttgtt tggaacttca attccagtgt tattattctg tatacttttt tttgcgattt
    22861 catatgtact aacaataaga ccttctaagg tcgtagatat agttggacag tttttaactc
    22921 cagctcttct aatagcttta gcatttataa ttataaaagg tattatatca cctcttggag
    22981 atatagtaga taaacctatg atacctaatg tatttgcaga aggtataggt caaggatatc
    23041 agacaatgga tgcttttgca gcaatagcac ttgcatcagt gctaatagta tctttaaatg
    23101 ataaaggata ttctactatt agtgacaagt tgaaaatgat tggtaaggcg ggtgttcttg
    23161 cttgtggagg attagctcta gtttatggag gattatgttt ccttggggca actgtatcaa
    23221 caatgtatgg aacagatgca gtacaatcac aagtaatagt aaatataact gaaggattgc
    23281 ttggaaatgt tgggaaagcg attttagcag tagtagtatc attagcatgt ttaactacat
    23341 ctataggtct tacatctgcc acaggtcaat atttctctag acttacaaaa ggtaaattga
    23401 gttatgaaaa aatagtatta gctgtatctg tattcagtgc agtagtagca tcttttggtg
    23461 ttggaactat aataaagata gcatctccta tactaagtat agtttatcca ccatcaattg
    23521 ttttaataat tttagctttc ttcaatgaaa aaattaagaa tgataatgta tacaaaggag
    23581 cagtatatat gtcattgctt gtaagtatac taacagttat atctagttat ggagtagctg
    23641 ttccagtagt aaattcttta cctcttaatt cattaggatt taactgggtg gtgccagtta
    23701 taatagctgg aataattggt aattttatac catcaaagag tcaatctaat acacttggaa
    23761 caaactaagt tttgtttaat taaaatataa tcaattgtaa ataaatgagt taactaaaag
    23821 taaaaggagt tatttgtaca aataactcct tttattttta gattctatat ttaatt
//
LOCUS       DACXMZ010000039        23477 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.039, whole genome shotgun sequence.
ACCESSION   DACXMZ010000039 DACXMZ010000000
VERSION     DACXMZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.039"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            134..1582
                     /locus_tag="JD016_06095"
     CDS             134..1582
                     /locus_tag="JD016_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HAU4999205.1"
                     /translation="MSILNLFRKNKNKNNVDCNEKSNESKQSYNDSTTLSLECNSNSP
                     HNIEVKNEINKVLNVSCNDTTNLYELEKQAREKSDALYDIHTNSINKFNPQNNILETD
                     TKPLTSIEKSFLKYIIGENIYEPYIATYWTYEYNINYSYLISKFFNMDYLKISNYIED
                     LTKLTVSELKEILKSNNIKSTGKKAELIERIEKEISCKDLSNFFNSSNKYYALTDKGK
                     ELLKDVRKSVTKNTDLENQCLELIYIDKYEEAYDLICKYESSKNIQRGININWENHKI
                     TPMKIESYKAIKELDINLKDTLLDNIIKSSYILCDMLGNNSKTSILVKRLVGEKIDNI
                     EINNAINSINDVIYNHSTITYNANDSINNKNLHYMDIVKNNKSIPFEVNNKNYNFKIN
                     ENEIYFFNILKIKMYENELKNNFVFDRMSDGAFNVFDTSDNFIGKVKLQGRKKWIMYT
                     KNEFGSEHIYGELIHLIDGIDAWIKYSKEYLK"
     gene            1853..3517
                     /locus_tag="JD016_06100"
     CDS             1853..3517
                     /locus_tag="JD016_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAU4999206.1"
                     /translation="MNNENEQLKSDLLINDEKIKNLNFKIPWYYSLWTISILILSTFS
                     TYSISFIVAIIFLFKRNKIMKKHKDSISILLSDVEKINNKYILLNDEIKMKEKHFEDL
                     CESNENKLKELSNLLDKKKVEIDKFDSENQDKFKLIEELKIEKERLDNLIKDKNILKD
                     NINTLNSHLEELKNEREELRDINTTLKNKKEELKRLSEELIQTEDEVLLQSFGLYNPK
                     YDFENSDEYMEKLKEIREMQKLLIRNKTGVKYSDSWTVDGSVQKGRTMTNQNIKTALK
                     LFNSECDIAMSKVSFKNIDSIEKRIRKAFTDTNKLNTSNKVSIKENYLNLKIDELYLY
                     YEYLQMKEEEKEEQRALREQMKEEALVQKEIENQKRKLKKEELQFKNELLRLKSTIPE
                     DENDKLEWEQKINSIEEKLALLSKDLDDVLNREQNTRAGHVYIISNIGSFGENIYKIG
                     VTRRLDPTERINELSSASVPFKYDIHATIFSEDAPKLESALHKAFDNKRVNKVNNRKE
                     FFKVTLDEIRTEVEKNFDKTVEYTKLAEAQEYRQTLKIQELNNKLA"
     gene            4031..5149
                     /locus_tag="JD016_06105"
     CDS             4031..5149
                     /locus_tag="JD016_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAU4999207.1"
                     /translation="MNIKSAFIRKRGTKFHVYVEYIEETTGKIKQKSYGSYEKKKDAE
                     KHLIEIKSTINNNKFITPNKTTLVERCYKYIIANEKNWSPYTTVNRKSWVNNYIEPFF
                     KDIKLIDINPSLLQFFINKSFNNSTSSSAKVRYNFLSSVLKEAYRLKEISENPCDFVK
                     LPAQNVTYEIEIYNREETLLLIEKLNNSIIEIPILLMLLLGLRIGEVAGLKWSDVDLD
                     NSIININQILIYANSKITFKEPKTAKSKRTLSVPKELIEKLKIEKLKQNKMKLQGTLE
                     NENNLVCLNTNLKPWIPAALSKTFHNFIKRNNLRNIRVHDLRHTNASLLLLGGTNMKV
                     VSERLGHTDIKITMNRYSHVLEEMDKEASDNLSKLLFK"
     gene            complement(5300..5980)
                     /locus_tag="JD016_06110"
     CDS             complement(5300..5980)
                     /locus_tag="JD016_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4999208.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(6030..8057)
                     /locus_tag="JD016_06115"
     CDS             complement(6030..8057)
                     /locus_tag="JD016_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAU4999209.1"
                     /translation="MVDTMKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIR
                     ASNGILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSM
                     RKGNQSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYR
                     PKIKNSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSII
                     CFTTFAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVE
                     DLSYFMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVN
                     IYGKLIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSN
                     RFEPIFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTL
                     LNERKRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRV
                     RSLQEFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFEAYILEKKIYAKINK
                     EMLTRAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRF
                     FTERDNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(8117..8794)
                     /locus_tag="JD016_06120"
     CDS             complement(8117..8794)
                     /locus_tag="JD016_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4999210.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(9374..9457)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(9690..10532)
                     /locus_tag="JD016_06125"
     CDS             complement(9690..10532)
                     /locus_tag="JD016_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAU4999211.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(10784..12568)
                     /gene="pepF"
                     /locus_tag="JD016_06130"
     CDS             complement(10784..12568)
                     /gene="pepF"
                     /locus_tag="JD016_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAU4999212.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(12634..13299)
                     /locus_tag="JD016_06135"
     CDS             complement(12634..13299)
                     /locus_tag="JD016_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAU4999213.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(13450..13665)
                     /locus_tag="JD016_06140"
     CDS             complement(13450..13665)
                     /locus_tag="JD016_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HAU4999214.1"
                     /translation="MFDKMYIKIYKFVIVVIIMANNELEALKNEIEAVREEINTYIEY
                     PEIFQEELVESSKKIDILINKYMYLSK"
     gene            complement(13689..14198)
                     /locus_tag="JD016_06145"
     CDS             complement(13689..14198)
                     /locus_tag="JD016_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAU4999215.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            14404..14622
                     /locus_tag="JD016_06150"
     CDS             14404..14622
                     /locus_tag="JD016_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAU4999216.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            14640..16586
                     /gene="feoB"
                     /locus_tag="JD016_06155"
     CDS             14640..16586
                     /gene="feoB"
                     /locus_tag="JD016_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAU4999217.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(16765..17820)
                     /locus_tag="JD016_06160"
     CDS             complement(16765..17820)
                     /locus_tag="JD016_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAU4999218.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(17868..18674)
                     /locus_tag="JD016_06165"
     CDS             complement(17868..18674)
                     /locus_tag="JD016_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAU4999219.1"
                     /translation="MTLNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIK
                     KLYNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTA
                     LMGPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKY
                     GYQVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVIL
                     QDILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(18661..19452)
                     /locus_tag="JD016_06170"
     CDS             complement(18661..19452)
                     /locus_tag="JD016_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAU4999220.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(19492..19914)
                     /locus_tag="JD016_06175"
     CDS             complement(19492..19914)
                     /locus_tag="JD016_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAU4999221.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(19930..20409)
                     /locus_tag="JD016_06180"
     CDS             complement(19930..20409)
                     /locus_tag="JD016_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU4999222.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(20541..23297)
                     /locus_tag="JD016_06185"
     CDS             complement(20541..23297)
                     /locus_tag="JD016_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU4999223.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         8276 a         3707 c         2677 g         8817 t
ORIGIN      
        1 tcaaaactat ttaattctat gttttttcct aatatacata gcatttatac agaaagtttt
       61 atatacttaa attgttattg tcacaaagta gaatacacta gaaattaatt aaatacaaaa
      121 ggagtaattt tttatgagca ttttgaatct atttagaaaa aataaaaaca aaaataatgt
      181 tgattgcaat gaaaaatcga acgaatcaaa acaaagctat aatgatagta ctacactttc
      241 actagaatgt aattctaatt cacctcataa catagaagta aaaaatgaaa ttaataaagt
      301 gctgaatgta agttgtaatg atacaacaaa tttatatgaa ttagaaaaac aagcaagaga
      361 aaaatcagat gctttatatg atatacatac taatagtatt aataagttta acccacaaaa
      421 caatatatta gaaacagata ctaaaccact tacatcaata gaaaaatctt tcctaaaata
      481 cataattgga gaaaatatat acgagcctta tatagcaacc tattggacat atgaatataa
      541 tattaactat tcatatttaa tttctaagtt tttcaacatg gattatctta agatttcaaa
      601 ttacattgaa gaccttacta agttaacagt ttcggaattg aaagaaatat taaaatctaa
      661 taatattaaa tcaactggta aaaaggctga attaatagaa aggatagaaa aagaaatctc
      721 atgcaaagat ttatctaatt tttttaatag ctccaacaaa tattatgctt taacagataa
      781 aggaaaagaa ctgttgaaag atgtgcgtaa atctgttact aaaaatactg acctagaaaa
      841 ccagtgttta gaattaattt atattgataa atatgaggaa gcatatgact taatttgtaa
      901 atatgaatcc tctaaaaata tccaaagagg aattaatata aactgggaaa atcataaaat
      961 tacacctatg aaaattgagt cttacaaagc cataaaggaa ttagatataa atttaaaaga
     1021 cacactttta gataacataa ttaaatcatc ctatatactt tgtgatatgt taggaaataa
     1081 ctccaaaact tctatattag taaaacgttt ggtaggagaa aaaattgata atatcgaaat
     1141 taataatgct ataaatagta taaatgatgt aatatacaat cactctacta taacatataa
     1201 cgcaaatgat agtattaata ataaaaattt acattatatg gatattgtta aaaataacaa
     1261 atcaatacca ttcgaagtta ataataaaaa ttacaacttc aaaattaatg aaaatgaaat
     1321 ctactttttt aatatactta aaattaaaat gtacgaaaat gaattaaaaa ataattttgt
     1381 ctttgataga atgtcagatg gagcatttaa tgtatttgat acttcagata acttcattgg
     1441 taaagttaaa ttgcaaggta gaaaaaaatg gataatgtac acgaaaaatg aatttggttc
     1501 agaacatatt tatggcgaat taattcactt aatcgatggt attgatgctt ggattaaata
     1561 ttcaaaggag tatctaaaat aataaaagag ctacttaaaa tgattaaaac taattttaag
     1621 tagcttttcc ttatgttcaa aatactgata acatactttt ataagaatgt tatattattt
     1681 ttataatttc tgttaacata cctttataat ccaactaaat tcttttaaat aactaccatt
     1741 ttgttttatt tttacctttt ttttcaaata tatggaaatt gcacataaaa ttttatatac
     1801 ttaaattatt attatcataa agtagaacaa aaaataaaaa aggagttttg atatgaacaa
     1861 tgaaaatgaa cagttaaaaa gtgatttact catcaacgat gaaaaaatca aaaatcttaa
     1921 tttcaagata ccctggtatt attctttatg gactatatcc atactaattc tttctacgtt
     1981 ttcaacatac tctatatcat ttatagttgc aataatattc ttatttaaaa gaaataagat
     2041 aatgaaaaaa cataaagata gcattagtat actattatct gatgtcgaaa aaatcaataa
     2101 taaatatatt ttattaaatg atgaaattaa aatgaaagaa aaacactttg aagatttatg
     2161 tgaatcaaat gaaaataagt taaaagaatt atccaatcta ttagacaaaa agaaagttga
     2221 aattgataaa tttgattcag aaaaccaaga taaatttaaa cttatcgaag aactaaaaat
     2281 agaaaaagaa agacttgata atcttataaa agataaaaat atacttaaag ataatattaa
     2341 tactttaaat tctcatttag aagaattaaa aaatgaaagg gaagagctta gagatataaa
     2401 cacaacttta aaaaataaaa aagaagaact aaaaagatta tcagaagaat taatacagac
     2461 tgaagatgag gttctgcttc aatcatttgg attatataat ccaaaatatg attttgaaaa
     2521 ctctgatgaa tatatggaaa aattaaaaga aataagagaa atgcaaaaac tattaataag
     2581 aaataaaaca ggtgtaaaat attctgattc ttggactgtt gatggaagtg ttcaaaaagg
     2641 tagaactatg actaatcaaa atattaaaac agctcttaaa ctcttcaata gtgagtgcga
     2701 cattgctatg tcaaaagtta gttttaaaaa tattgattct atagaaaaaa gaatacgaaa
     2761 agcatttact gatacaaata aattaaatac ttcaaataag gtttctataa aagaaaacta
     2821 tcttaatttg aaaatagatg agctttattt atattatgaa taccttcaaa tgaaagaaga
     2881 ggaaaaagaa gaacaaagag ctttgagaga acaaatgaaa gaagaagctc ttgttcaaaa
     2941 agaaattgaa aatcagaaaa gaaaactaaa aaaagaagaa ttacaattca agaatgaatt
     3001 acttagacta aaatcaacta tcccagaaga tgaaaatgat aaattagaat gggaacaaaa
     3061 aattaattct atagaagaaa aactagcttt gctatcaaaa gatttagatg atgtattaaa
     3121 tagagaacaa aatacaagag caggacatgt atacataatc tccaatattg gtagttttgg
     3181 agaaaatata tataaaatag gagtaactag aagattagac ccaactgaga gaataaatga
     3241 attaagtagt gcatcagttc cttttaaata tgatatacat gcgactatat ttagtgaaga
     3301 tgcacctaaa ttagaatcag ctttacataa agcttttgat aataagagag ttaacaaggt
     3361 aaataataga aaggaattct tcaaagttac gcttgatgaa ataagaacag aagttgagaa
     3421 aaatttcgat aagactgtgg aatatacaaa attggcagaa gcacaagaat atagacaaac
     3481 attaaaaata caagaattaa ataataaatt agcttaaata tattcaaaca attatacttt
     3541 aataaaatat tatttttgaa tatctatacc aatttgtgat ataataatag caaggagata
     3601 aaatttactt tttaaaaggt gaatttctct aattgattag aatttaaaag ttatttcttt
     3661 aaatcaccct tattggcgtt tgggtgattt tttattttgt catatatgaa agctgataca
     3721 ataccagcta ctatactcaa taaaaagtta attaacatat aaattcacct ccttcctata
     3781 ttggaatttg gcgtttatat gagaaaatca cccttcgctt ttcaatttta ttttccttgc
     3841 taaaaatatt ataacatata attcttacat attttaccta tatattactt ttttaaagat
     3901 tcgtctatat atttctgtat acatctactc tatcatcgaa tagaatttat aacttagatt
     3961 tgatataata aaaccatagt tattcaatat attttaaaca tgaataatac ttataaagga
     4021 gtgtacatat atgaatatca aatcagcttt tataagaaaa agaggaacaa aatttcatgt
     4081 atatgtggag tacatagaag aaacaactgg caaaataaag caaaaaagtt atggaagcta
     4141 tgaaaagaaa aaggatgctg aaaaacattt aattgaaata aagtctacta taaacaataa
     4201 taagtttata actccaaaca aaacaactct tgtagagaga tgctataagt acataattgc
     4261 aaatgaaaaa aattggtctc cttacacaac tgtaaataga aaatcttggg ttaataatta
     4321 tatagaacct ttttttaaag atataaaact tatagatata aatcctagtt tacttcaatt
     4381 ctttataaat aaaagtttca ataattctac ttcctcaagt gcaaaagtca gatataattt
     4441 tctatcttct gttttaaaag aggcttatag gctaaaagaa atatctgaaa atccttgcga
     4501 ttttgtaaaa ttgccagctc aaaatgttac atatgaaatt gaaatatata atagggaaga
     4561 aacattattg ttaatagaaa agttaaataa tagcataata gaaattccta ttttattaat
     4621 gttgctttta ggtttaagaa tcggagaagt agctggtcta aaatggtctg atgttgatct
     4681 agataatagt ataataaata ttaatcaaat tctcatatat gcaaatagta aaataacttt
     4741 taaagaacca aaaactgcaa aatcaaaaag aacgttatca gttccaaaag aattaattga
     4801 aaaactaaaa atagaaaaat taaaacaaaa caaaatgaaa ttacaaggta cacttgaaaa
     4861 tgaaaataat ttagtatgtt taaatacaaa tttaaagcct tggataccag ctgcattaag
     4921 taaaactttt cataacttta ttaaaagaaa taatttaaga aatattagag tacatgattt
     4981 aagacataca aacgcaagtt tgcttttgct aggaggtact aatatgaaag ttgtttcaga
     5041 aagattaggt catacagata taaaaataac tatgaatagg tactctcatg ttttagagga
     5101 aatggacaaa gaagcttctg ataatttaag taaacttcta tttaaataat aaaaactgac
     5161 tgtcagttaa atgtcagttg aacctaaaaa aataggtttt gtcagttaaa tgtcagtcag
     5221 ttagtttata ataatttctt ttaaataccg ctaaaactgc acattttaac acttatttat
     5281 gtttgtttat acgtatttat tattctttat taaaataata tcctgctcct cttattgtta
     5341 taattttctg aggattttta taatctttct ctattttctt tcttaatcca ttcactgcaa
     5401 ctaccacact atagttatcc tctaagtatt cattcatcca tacatgttca tatatctctt
     5461 tagtagtcaa agtcttttca ggattctcta taaaaaagtt taacaactta ttttctgttg
     5521 gagtcaattt tattatttta tcttctaact ttattactcc actagttcta ttatattcct
     5581 ctaattttgt attattttca ttattattta catatgagtt cattcttctt aaaatagctt
     5641 ttacttttgc atataatatt tctaaattta gaggctttat taagtaatcg tctccaccag
     5701 aatttaaagc atttaaaatg ctttggtctt catttatata agtcatataa acaattggac
     5761 aagtagttac atttctaatt tttttacaaa gagtccatcc atcaccatca cttagaatta
     5821 tttctaaaaa tattaaatca tatttattgc taaatatttt tccaatagct tcttcacaat
     5881 tatatgctaa atctatactt attccttttt cctccagaaa tccttttagc ttctggcaat
     5941 tatccttgtc tccatctatt atcaatatct tagtttgcat tataatcact cccaaatatc
     6001 attattcttt tatattaaat aagctattat tataaaaaac atagacttaa ttttattgta
     6061 aaaacagttc cttttcctag ttctgaatct acaaagattt ctccaccatg ctcccttata
     6121 atggatttga caatatatag ccctaaccct tgcccttttg gctcattatt atctctttct
     6181 gtaaagaatc tgtcaaatac ctttggtata ttttctggag gtattccttt tccattatca
     6241 gaaattttaa taattgcaaa tccatcttct ttatccattg ataaaattat ctttccatca
     6301 aatggagtaa aggtcaaagc attataaatt aaattttcaa atgctcttgt aagcatctct
     6361 ttatttatct ttgcatatat ttttttctcc aatatgtaag cttcaaagtt tacaccacat
     6421 gcttgtgtat ctggcaaatt attttcatat atctctttaa taaaattatt cagacatatt
     6481 ttttcactaa taccaatgct tttatcttgt gatgtaaatt cttgcaaact acgtactcta
     6541 ttttgtactt catttgactt ttgatttata gcataaagat aggttcttgt ctcttcatct
     6601 acatgaatat taccaacctt tatcaaatct ataaatgctt gtatagccga aacaggtgcc
     6661 tttaaatcat gtgctacatc tgccaagaat cttttacgtt catttaacaa tgttgtaagt
     6721 tcattagttc tcacttcaac ttcatgttca agattccttg taagtttttc attctctttt
     6781 attattattc tattcctgtt tatcatcata acagaaaata aaataacaat tacaaaccca
     6841 caatattctg tttgccaccc tgtaaatata ggctcaaatc tattactcgt aactgcatca
     6901 aataatatgc taattccaaa aacagtgttt actgaaacta aaatataaga acttatatgc
     6961 tcagagcatg tctttttata tattgaaacc agcgaagaca ttaatatata tataaataca
     7021 atatatttat atatgtctat taacttccca tatatgttga caaagtacat atcatatgga
     7081 agaagaataa ttggtatcac tatagatatg atactcattg taattcctaa tggtaaaatt
     7141 aaaaatctat acactttatt ttcttctatt ttggatatac ttccatttat acaaataata
     7201 cataaaacta ccataaaata tgacaaatct tcaaccgcat atgtaaagct taccaaaggc
     7261 agtcctaaca aatgtacaaa gggataggat acatggatgg caaagaaaac acacatacat
     7321 ccaaaatata agtaaatttt acttttgttt gaaagaaacc atacagacaa tgaaaatata
     7381 gctattgcaa aagttgtgaa gcaaattata ctataaaaaa gtaatcgata aaacatcata
     7441 ttacttatgt cctttgtttc tcctaatgca ggcggataat acaaaccact ataataatgt
     7501 gaaaaattcg atacattgat tataatttct gtatttttct ttgcaataaa acttactact
     7561 gaatttttaa ttttgggtct atatttttct gttcctacat ctcctaattt agaaaccatt
     7621 tctccattta tccatacttc acttgcggaa aaaatctcag gaagctctaa acttagtagc
     7681 ttatccttgc cttcattttt tattctcata cgataagtag cattcccata tggtgactga
     7741 ttcccctttc tcatagaaaa atctggatat tgacctataa aaacatattc tggttttata
     7801 tcttgctctg taaaatcatt cggtttaaat attttatgat tataaaactc ccagtcatct
     7861 atcaaaaaga caagtttacc tttagtgaaa gtatcattat ctatagttaa aattccatta
     7921 cttgctctaa tagacttata tgtatattta ttatcatatt tatacagaag atttaatcct
     7981 gttattgcca acagagagac tatcaataca atgagagttc cttttagtat attatacttt
     8041 ttcattgtat caaccacatc tctttcatag tagtattaaa taaaacaaga actatttatt
     8101 aattacagta tctcacttat ggtacaaact tatatccttt tccccatacg gtttgaatgt
     8161 attgatgctc cctgcttaaa gaattaattt tttttctcat ctgtgcaata tgaacctgca
     8221 ctgtatgttg atctccaata tcaggcattt tccaaacttg gttatatatc tctatcttcg
     8281 aaaatacttg ttctttgttt tcagctagaa acaataatat ttcaaattcc atagtagtta
     8341 aatctatact ttctccatca aaagttactt ttcttgaatt tacatttatt actaacgctg
     8401 aaaactttaa cacattagga actgatgtaa tatttttgta ctgcctcata cgagcatata
     8461 ttctaagttc aagttctttt aaactataag gttttgttat ataatcatcc cccccagcta
     8521 aaaatccccg aactttttct tcttcatgct cataattaga taaaaatatt ataggtatgt
     8581 taaccttttc ttttatatca ttgcacattt tatatccatc tatatctggc aatgaaatat
     8641 ccaaaattat acagtctaaa actatactct ctactatatc catagcatct ttagcagtct
     8701 ctgatgtata tacattaaaa cctatattag aaaaataaat ttgatttatg tttaaaatat
     8761 caacatcatc atctacaacc agtaaattat acattacacc actccattca aaggtatttt
     8821 aattataatt ccaagtttta tagttattac aaattataat gttttatttt catatataac
     8881 tggtattatt attttataat atctttacaa actagactat aatacttaag tatttttgaa
     8941 taattcttga atattgtatt aaacattaag attcattatt aaatactaat taaagtctcc
     9001 gattgttgga gaatggagct taaagtcatt ttcacgtata aatttattat ttttctagaa
     9061 acttgtctat tggctagttt gagtattgat ttatatgatt tatatggtta taatataaaa
     9121 taaattgtat tattttggta aatatattgt tacaaaaggt ttatattctg catctcaatg
     9181 tttataaaaa ctttattttc ttattataat actattattc ttttaaaaaa atttcccttt
     9241 tccaacaaat ggctacttta aaagccaaaa ttttacctat caataaaaat cttaacgact
     9301 aatactctta ttttcaaatt ttgcaacata tttgttaatt ttaaaacaaa aaaagactat
     9361 gcaatataaa aattatattc atagccggtt gcaccactaa ctccatatat ctctagatgc
     9421 ccaataataa gatatacttc tcagtttcta aaatattttt attaaaaatc ttttatatat
     9481 tttcataaat ttctttttta ttctaacatt gtaatattat tttgtcaata tttccatatt
     9541 cttagagata agatactaaa atacctattt tatctaaaga tatttatatt tctacattca
     9601 ataatagaca ataccctgca agttaaagca tcatttttat gagcttatct cacagggtat
     9661 tatatgttta ctttatatta taactcttgt tattgattaa ctttaataaa tcctttttct
     9721 gaaacaagtg cttgtccttc atcacttaat atataatcta ttaaagcttt acctttttct
     9781 gacaaaccat cttctttatt aactaatata aatggtcttg atattggata tttattagct
     9841 attacatttg catcagttgc ttcaacatca tttattttta aactatttac tttctcatca
     9901 acatatccaa atgatatata tcctatagca ttttcatttc cttcaactgt agtttttata
     9961 tttcctgaac catcacttat ttgtgcatct ttagttagtt cttctgaatc aaaccctaca
    10021 atttcttgga atccatctct agttcctgaa ccttcttctc ttgatactat tactattggt
    10081 gcatcatttc ctcctacttc tttccagtta gtaatctttc ctgtatatat acctttaact
    10141 tggtccattg tcagattttt tactgagttt gcagtattag ttatcaatgc tataccatct
    10201 attgctattt tagtttcctt taatccagca cttttttctt catcttttaa atctcttgaa
    10261 ctcattccta tttcagatgt tccttctata gcatttttta tacctccaga agaaccaatc
    10321 tgttgtactt ctacactaac accttgattt ttttcttgaa acttttctcc gatagtttcc
    10381 ataagaggac ctattgaagt tgaacctgaa acagaaactt tgcttgaatc tcctgaatca
    10441 cctgaacctc ctgagttaga actacatcct actgctaaag ctccaactaa cattgtacta
    10501 gctaaaagaa cgcctatttt ctttttaaac attttactta cccccgctta tattttaatt
    10561 ttttgttgtc atttttctat attttaataa tacagattct ttgttaaatg aatattagag
    10621 cagggttaag taaatgtaaa tattgttaaa tacatgtaaa aacttaattt tatatgattt
    10681 attcttaatt agattaactt tttaaagtta ctttaggcag atataaacat aatacaactc
    10741 atattatcag aagtcttcat tgaagctatc aacctatcac atcctataat tctttttcta
    10801 gtttattaac taattcagca aatacattta aggcttcctc tatagattct tttttcgtca
    10861 tatctacacc agcagatttt aattgttcta gtgggtattc tgaccctcca ctttttaaga
    10921 agtttatata tctatctact gcagtacttc cttctgaaag tatctgttga cttaaagcac
    10981 ttgcagcaga aaaccctgtg gcatacttat atacatagaa gtttgaataa aaatgaggta
    11041 ttctcgccca ttctattcct atttgtttat ctactattgc agattttccg taatactttt
    11101 catttaattt gtagtatata ttagtaaatt catcagcagt aagtggttct ccactctcta
    11161 ccctttgatg tacaattttt tcaaattcag caaacatagt ttgtctatga actgtagttc
    11221 taaattgttc taaataataa tttaataagt atatagtttc atccttagac ttagaatttt
    11281 ctaataaata atttataaga agtaattcat ttaatgtaga tgcaacttct gcaacgaata
    11341 tcttatagtc cgaatataaa aatggttgac ttgttcttga ataatggctg tgaacagagt
    11401 gccctaattc atgaattaaa gtgaatagag aattaaggtc atttttatag ctcattaata
    11461 tataaggatg tgaatcataa cttccccaag aataagctcc tcctttttta ccttcattat
    11521 catatacatc aatccaccct tcatcaaaag cactttttat gaccttcaag tattcttctc
    11581 ctaaaggttt aagtgcctct aatatgatat cctgtgcttt atcatatggt atatccatgt
    11641 caaatttatc tgtcaaagga acatacaaat catacatgtg tatttcattc aatcctaaaa
    11701 atttcttttt aagctcaaca tatttattta gattaggaat acttttatct atagctgata
    11761 taagattatt gtaaacatct aaacttacat catctgaaaa taaagacgct tctattgctg
    11821 aattatactt tctagttttt gcgtaaaata tctctgattt tattccacca taaagcgttg
    11881 aggcaaatgt atttttatat ttctcatatg ttgaaaattc accttcaaaa gcgtcttttc
    11941 taactctact attcttactt tttataaata aagagtagtt tgaatgagaa agcttaacct
    12001 tattcccctc ctcatcttct atctcaggaa attccatatc tgcatatgct aacatatcgt
    12061 atacattttc tggaattgtc gataattctg atgtagctgc aagtatttct tcctctcgtt
    12121 cactcaaagt atgtggttta tctctcaata tatcctttat atattttgca taaaaggata
    12181 gcttctcatc ttttaagtat tcacttaact tattctcttc tatcgccagt atttctggaa
    12241 ctatataaga agtagccaca cctaaatcag tagataacat ttctgtctta gtggctcttc
    12301 cttgattatc attaattcta gtatcttcat gttgtttcat atgagtatat acatataaat
    12361 tttgaacaat cctagatgca ttttcagaga tatttaatac tttatataag ttctccttac
    12421 tctcagaaag cttcccttta tattctttta cttcttggat aagatttttt actttactta
    12481 tgtctttttc aatttcctct ttactggagt acatcttatc tatcttccat ttgtactttt
    12541 cttctattgt acttctatca gttcccatat catacacctc tattttaatt tgttttatat
    12601 tttattcact ttacaaaata tattgtatat attttagtcc aaataatcta catttattac
    12661 tccttctatc atatttattt ctttcataac gtcgtgtaag tgtattctct tagcataaga
    12721 tacaaaaaaa ttaaccttcc agtattcttc attacgacat tctatttcca tatttcttac
    12781 gataagttga tttttttcaa atttatcata taaaaaaggt atagaatcca aattgcttaa
    12841 ttttaatatc aatacattct tttcttggct aaataaaaac gtattgatta tcctaagcac
    12901 aaataatgct atcattacaa ataatccacc tagaattgcc aattcataat aaccatatcc
    12961 aactgctata ccaatacatg cagttgccca catcccagca gctgtagtaa gcccttttac
    13021 tccaccttta ttttttaaaa ttgctcctgc acctaaaaaa ccaattccag ataatacttg
    13081 agcaggcatt cttgctgggt cagcatttgt atacaaatta taatcaaaga ataattgaat
    13141 cgaagttatt gaaacaatac aagaacctat tgcaattagt atatgtgtcc taaatcctgc
    13201 aaactgtctt attttttctc tctctaagcc tattattcct cctattatta gtgctaatag
    13261 taaccttatt gctatctcct tacttcctat atcaatcata atataaaacc ctcctgactt
    13321 aattacaact tatgtcctac agataattta cacatcagaa atactatttt atatcttaat
    13381 attattatta atatatgtaa aatatagcca tctgttaatt tttgatacaa aaaaacccac
    13441 aactgtgggc tatttgctta ggtacatgta tttatttatt aatatatcta ttttcttact
    13501 tgattctact aactcttctt gaaatatttc tggatattct atgtatgtat ttatctcttc
    13561 cctaactgct tcaatctcat tttttaacgc ttctaattca ttgttcgcca taataatcac
    13621 cactattaca aatttgtata tttttatata cattttatca aacaatatta tattttgtca
    13681 atattatgtc aataaaactt actataaaaa tgctttaatt taggtattgt atacctggat
    13741 actaagccta caaacatacc agttactaat gatgccatta acataaatgg taagtaccct
    13801 atagcaagta tattttctat gattaaactt gcaactatta attgacctat attatgccct
    13861 attgcaccta taatgcttat ccctattagt gataaatctt ttattttact cgctaaatac
    13921 ataaataaaa gactcagata agctcctcct atactaaata gtagatatga taaagggcct
    13981 gcaaatattg atgacaaaat taccctgata gtaacaatta taacagtatc tttgacacct
    14041 aacaacatta atgaaattaa agttataata ttgctcaaac ccaattttgc ccctggaaat
    14101 ataactataa gaggatttgg tatatatgtc tctaatacat ataaagctaa actataccct
    14161 atcataagac ctaaaaatgt catttttcta gttttcatta agttaactcc tccaaatttt
    14221 atctatcatc ttacattata atacaaaata tataaaaaca cctaatttag actaaaatat
    14281 ttttaatcta tttagaataa atattagtta tttcaatgtg caattacctc tataaatctt
    14341 gactttttgt atcatacttg ttacaatttt aatgataatg aatttcatta ggaggtgtta
    14401 ataatgactg tttacgattt aaaaatagga gaaaaaggat ttattaatag tatagatgga
    14461 gatattaaat tatctaaaag attgcttgca cttggatgta taaatagtac tgaaatagaa
    14521 gttaaaaagg ttgccccact tggagaccca attataatta gatttagagg ctttgacctt
    14581 gcgattagaa aatctgatgc taaaaatatt tctttacaat aatagaggag gagctacccg
    14641 tgattaatgt agcattatta ggaaacccaa atgtcggtaa aactactgtt tttaatctac
    14701 ttacaggttc aaatcaatat gtaggtaact ggcctggtgt tactatagaa aaaaaagaag
    14761 gatttcttgg aaaagagata aaggttgtcg atttacctgg tatatatgca atggatactt
    14821 tctcaaatga agaaaaggtt tctaaatcat atttagaaaa tgaagatgtt gatgtaatag
    14881 taaatgtagt tgatgcttct aatttatcaa gaaaccttta cttaacaact caacttatgc
    14941 aatttaacaa gcctatagtt atattgctta atatgttaga cattgcagaa tctaaaggag
    15001 ttaatataga tgccaaaaaa ttaagtgaag aacttggagt aattgttgtt ccaattatag
    15061 caaaaaagaa aaatggtgtt gataggatag aagaagagat taagggagct acgcaaaaag
    15121 cttttatata tcagaaaaac tttggaagtg aaacagaaac atataaaaaa ttagaatcca
    15181 ttttatcgag atgtacaaaa actacatata cacaaaagaa atctatcagt gataagatag
    15241 acaatatagt cttgaatcct atattagcat atccgatatt tattggtgta ttgttcctct
    15301 tatttaaatt tacctttgac tgggtcggag gaccactaca agagggattt gctagtctta
    15361 tagaggctta tatttctgca cctgttagtg atatgttatc taactctagc ccatggttta
    15421 aatcacttat agttgatgga ataattggag gtgttggtgg tactttacca ttcttccctt
    15481 taatatttac attattcttt ggtatatcgt tgtttgaaga tagtggatat atgtcaagaa
    15541 cagcattttt aatggataaa gttatgagaa aggttggtct atctggtaaa gcttttatac
    15601 ctatagttat gggtatggga tgttcttctc cagctataat ggcaactaga acattggaaa
    15661 gcgaaaaaga tagaagagtt acagctctta ttgcaccact tatgacttgt ggagcaaagc
    15721 tacctatata tgcactattt gtagctatat tctttccaca taatgctgct ttagtaacta
    15781 catcactgta tttggttggt atagttatgg caattgtagt tgcattattc ttaaataaaa
    15841 cagcctttaa aacagaggct gaaccattta tattagaatt accagaatat agaatcccta
    15901 ctatagatgc acttattaaa aatacttgga ataaatcaaa aggtttcttg ataagagttg
    15961 ttactgtcat gtttgcaatg tcagtcgtaa tttggggact ttcttcattc aatgtttttg
    16021 gatatacaga agatataaat gtaagcttct tggctactct aggtcatatt atatctccta
    16081 tattcaaacc tcttggtttt gatgattgga gaacttcagt tgctatttta agtggtcttg
    16141 gagcaaaaga aatcgtagta aactctttaa atatactata tggtaatcta actgttgtct
    16201 taccaacaat atttaatgga gttactgcat atacattctt gatatttact gctttatata
    16261 ctccatgtat agctgcactt gcaactatga aaaaagaata tggcaataaa atgatgttta
    16321 catcatttgc atatcaattt atactagcat ggataatggc ttttatagtt aaaagtatag
    16381 gtggagtgct atttatgggt aattcaataa tggaagcttt gataggtgga ataattatag
    16441 tccttgcact ctttattctg tttaataaat tcaagtctac aaaaaacgga aatgtctgta
    16501 gtggatgttc tatttgttct ggatgtccaa gtgctaattc ttgctcaagt aaggaagaca
    16561 gcaataaaaa aaataatgca cactaatata taaatatttt agtttaaaat aagataaaag
    16621 tgggtgtctc aaaattaact ttttagttca tgaggcactc ttttttatag aaaatcaaat
    16681 atattttcat catttcaaca gtaaaaaaga gcttatctag taaaaaagag gcttatctct
    16741 attttgagac aacctctttt tttgttactt attcagtttc ttgctaccta acttcatgtg
    16801 gaacctagta tcttttggaa cacttgggtc tacacctctt aatgtaggta tttccccaca
    16861 tattagttgt ataggtacta taaaattaaa tgtttgatag taaggattat ctgtgatatc
    16921 aatctttaaa tttttatcat tttctgtaac atctctacct atagcaaata cattttccgt
    16981 ccaaccagaa agtacatcaa tcatcttagt tactctaggt tcttttcctg tatctagtat
    17041 aaatatagta gaatcactat ttatagcatt atatatacca tgtataaact cttcaaactc
    17101 atacccagtt acaggtattc tcattgtttc caataatttt aatgctgctt ctaatgtatc
    17161 tccatatata tcagagtgac ctattattct tatttcttta gaattaacta acttttcttt
    17221 gtttctttca atccattgtt tagagagctt atacactgct tcaaatctat taattgcatc
    17281 taatatctta tttatttcct cattgtactt ttcactagat attatacctt tttctctagc
    17341 tatttgaaga cctaataaca ttaagtttaa ctttgtacaa taatatcctt ttgttttagc
    17401 tccagatttt tcttcaccac aatttactgt taatatataa tcagaaatct catctattaa
    17461 agcattctta caacctgcca tagatgctat tttacagccc ttatcttcag ctaacttcat
    17521 agcgttgtat gtagaataac tactcccacc ttgagataca cctactacta gggtattttc
    17581 attatcaaac ttgaatgtat cttcagttat cataaatgga tacattttca caacatctat
    17641 atctaataaa ttttgtaaat atggttgcac ttgcactcct gagtgatagc tagtacctga
    17701 acctgttatt ataatctttt ttagatttgt tctcttaact tcattaaata agctatctgc
    17761 attagatata atttctctca ttctaactgg tgtttctaac atataatctt gtattgtcat
    17821 tttatacacc ttccttatct atatattcaa taaattataa ttaatttcta taacaaacct
    17881 atagcatgta aagcgattcc tgttactata gtgcctatag ttaattttgt tacacttacc
    17941 tttctcttta acatgtagta tattacaaat gcaaaggtta aaggtaacat attaggtatt
    18001 attttatcta gtatatcttg taaaattact tctgcaccat ttaagttgaa tttcagagga
    18061 gtagttatac tcaacatagt tgcaaccatt cctcctacaa cgaataaacc aacagttgta
    18121 gccatagaca ttattttctc cataagtccc tctctttgta ttctttcaag agaatttacc
    18181 cctacttgat aaccatactt aagtccaaat attctaaggg caaatgctgg tatattgtat
    18241 aatagtaaga ataatagtgg tccaaagata ttaccttctt ttgcaagtga tactcctact
    18301 cctgctgcta taatcctaaa tgttccccag aataatgaat caccaattcc tgcaagaggt
    18361 cccataagag ctgttttaac agcactaatt gaactttcat caaattctgg attattagca
    18421 ttctcttctt ccatagctgc tgttatacct aatatagcag gaactactac tgtcgttgta
    18481 ttaaaaatct ccaaatgcct ttctattgct tttgcttgat tttctttctt attgtatagc
    18541 ttctttattg caggtaacat tgaataacag taacctaagt tttgcattct ttcgtaatta
    18601 aatgcacctt gaagagcaaa tgaacgccaa aacatacttc ttaattcttt ttttgttaat
    18661 ttattcaaag tcaatctctc cttccgttaa agtattgatt gctaattgag gattttgatt
    18721 attttctttg ttattgatat aaaagtttag tacaaatgct acacatactc caaataaagc
    18781 tatagcagtt atatctaatc caccataaga tgctaacaaa aaccctataa agaaaaatac
    18841 agacatcttt tttgaaagta acatatccat aagcaatgca aaacctactg ctggcaacaa
    18901 tgttcctgca acacccagtc cgtctaatat aactttaggt attgcattta tcaatgctgt
    18961 aactttatca gcaccaatca taattgctaa aaaggttgga actgatgtac ttaaaaagaa
    19021 taatattact ggcaaccaca tcattatggt aactttctta aaatcagctt gtttagcata
    19081 gaaaactgcc ttttgagaaa agtacgaatt tattattcta actaatactc caagagattg
    19141 tgctaagact gctactggta ctgctactgc taaagcaact tctgcaccat tacctgataa
    19201 tatagcaaaa gctgtaccta atacgcctcc tgttactaca tctggaggag tagctgcacc
    19261 tattcctgca attcccatcc aaattaattc aagctgtgca ccaatcataa ttcctgattt
    19321 tatgtctcct aaaactaatc ctactaacat acctgtaact attggcctat caagcataga
    19381 ttgaccaaat atacgcccat ctagtatacc aactcctgct ataatagcta acatagtagc
    19441 ttgtactaac atattgaacc tccattttaa cttagtttaa tttacattaa attacattac
    19501 atttttgatt ttgttttgat tttctaataa ctcatttaca taaacaattc cattttttgc
    19561 ttcctctaat cctgatttaa ctattagttt tgtatcctcc tcaactaata aaagtataga
    19621 taaaagcatt ggtaaattta ctcctgtcaa taattcaaat ttgaaccctt gtaaaagtaa
    19681 gtttgtacat atgtttgcca cacttccacc aaaaacatct gttaagacaa caaattcttc
    19741 actatcttta ttatttgata atatacttct aatttctttc tcagcatcat ctgctgattt
    19801 atccgttgtc atacaaaaat actcaatatt agttaatgaa ccaattatta atttacttgt
    19861 gtcaacaagc tctctggaca attctccatg tgttgcaatt agaaattttt tcattctctt
    19921 tctcctaaat tattttatta aatctgatag ttgtttcttt ttgtctctag gtaacattct
    19981 aaattctata tcaatcccta aatcacctat ttgtttaaag ttatttatat catcatcatc
    20041 aacagcaact aaatctgtaa gtttcttctt cccttcctca tatcttaatc ctcctacatt
    20101 tatttctttt aatactcctc ctgaattttt agctacaact aaagcatctt tggtattttt
    20161 tactaacact aatacattgc ttttctttga agaagcaaat ttttctacta tttctccaga
    20221 ttcctctacc ccccttatat ataaagttac attgctaggt ttcgctaagt tcagtgacat
    20281 agctttcatt tggtcatttt ttgcttcatc atttgcaact agtattacat ttatacctag
    20341 tgatgttgcc catccatatg caacctgacc atgtatcaat ctgtcatcaa ttctcatcaa
    20401 agataccatt ttccccaaat cctccatcat acattaaaat ttttattgtt tatactctca
    20461 tttatctata aaagcaaaaa gtatgccaaa tactggcata cttttttaaa tttcattcac
    20521 ttattttgta atcttgtatt ttactgtggt agaccataga taatattata aatatatcct
    20581 atctctgaaa ttggtatttg cacactataa ctcttttcta gcacactaaa tgcatttttt
    20641 attagtttta cttcactatg atggtttttg attaacaaat ccatatcagt atactcttca
    20701 atttcagttt gtctaactaa tctttcaatc atacaactta agtgaacata taaagaaatc
    20761 tttttatcat ttgaaagttt tttcttttga atattttcta tagcattaat tccttcttct
    20821 attctctcta ttatcttttt tgaatctaaa atagttagac tatctattac tctaattaat
    20881 gaaaaattct ttacaataga atcatttatg ttctttattt tttcattttc tactactctg
    20941 ctaaatattc taaaaacatc atcttctcct tttcctgata ttatatcctc taataatata
    21001 aaatctaccc cattcacatt tggattggca gttcctacta tagccatcac atcatacatt
    21061 tcaaaaactg gttctttatc tcccatagaa acaagactat cataatcata aggtattatt
    21121 ttgacatcaa ctattccaat taaactttcg gaaagtagat gttggatttt tatagcagta
    21181 cctatccctg taaaacaaga tgatattata gcatattctt tatttttttg aggataatac
    21241 aattgtatat taggtttagt ttcttcatcc ataatttctg caatctcttc aatgttttta
    21301 ccttgcatta ataaatttcc tacttctagt gcagaaatag tggatatttg gtctataaat
    21361 aacattgggc tatttatctt attctttaaa tcttcatata taagattaag agagcccata
    21421 tcaaataaca caattaaacc attctttatc ctatctccct ctaggtatct agacatttgt
    21481 tttgatacat cagcaatagt agcatctata ggcatatcaa tagctgtaaa tatattttta
    21541 cccaacatcc gattacatac attcgccata ctactggcag tagcatatcc atgtgctata
    21601 atagtcgcat taacttgatt aaatttcatt cttataattg ttgatttaat aaatataatc
    21661 attatgattg tatctacatt attaagatac atatccaatc tattcgttat taatgttgat
    21721 attttactta ttatagagta ttctttgtaa ctctcttttt ttaaatatat tagtaattta
    21781 ttttctaaag ctttatactc atcagacaat tgataagatt cgtccttcaa ataaagataa
    21841 gacgaaaatg ccaatacatt attcccatca tattttatat tgttattaac ttctaaattt
    21901 ctaaatatat cctgcattat cgatattata agctccataa gtacgttatt attttcatca
    21961 ttatttgaaa agactaaatt gtccattagt ctaatcatta gattagaact ctttttacta
    22021 aatttttcct ttgttatttc atttttttct agtctatcaa agtaatctaa acattttatg
    22081 aaactttgat agattatatt tcttttattt cttccatttg acatttcaat actaaattgt
    22141 ttaggagaaa agataatgct tttgctttta ccactttgga attgaaatat atctacattt
    22201 tctatataaa gatttctagg aaggtcttga agagtaacaa aaatatttcc atttcttctt
    22261 tgattttttg cataagctcg tccacatgca tattttatta catttttaca ttcacctata
    22321 ttccccttga attttatctt taaaatcgca tttaatgtaa tttgagttat ctctataggc
    22381 aaaccaagta ccttactttc ttcataaata aaatgttcta caatcttcct tttttcaata
    22441 gtccctcttt cttctatatt gggaacataa atatttatgg gtattcttct taaaaaggtt
    22501 tgtaaaaatt cagatagact ttctgttgta gcaaatatta atcttaccct agcttttcta
    22561 attactccat tctctccaat cctagaaaac gtacctgtat ccataaatgt aaataacttt
    22621 tcctgacctt cactatttaa tctatgtact tcatctaaaa ataaaacacc accatctgat
    22681 tgttctatta gacctgctct agattcattt gctccagtat atgctccttt tacatgccca
    22741 aataatagac tagacaataa ctccaaatta ttataatact gcgcacagtt taacacattg
    22801 tatggagaat ttttttctaa tacattactg cttacactat atttgtatat tatcttggct
    22861 aaaaaagttt ttcctactcc actttcacct gtcagcatta ttgtaagtcc gccatttgga
    22921 taaagtacag cggtttttat ttgttcgatt acttctttta agctattatc atatcctatg
    22981 atactattaa acacagtctc ttgctttaat aatttacact catttttaag ttcatctaaa
    23041 ttttcataaa tacttttttt tgtaggtcct actacatcat ttattttatt tttaggtaaa
    23101 aaaataacag gtctactgtt tatctttata agattatcag attttactaa atcatttaat
    23161 aatctacttg attgagttct acttaaatta aactttttta ctatatcttc tgttattatg
    23221 ccactttctt ttttaagttc atcaaagttg ttaaataata cacattcatt tacaaaactt
    23281 aaaatttcat ccctcatgtt caacctccaa tacaaatatc aataatacta taatattagt
    23341 atatcattta tatatttatg aaaaagataa ttatattcct atcttgattc tcttttgtaa
    23401 ggttgtaaaa tcaaactttt tataattata aaatctagat tttttaacat aatctcaagg
    23461 ttcttatctt tccatat
//
LOCUS       DACXMZ010000040        23220 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.040, whole genome shotgun sequence.
ACCESSION   DACXMZ010000040 DACXMZ010000000
VERSION     DACXMZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23220
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.040"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1085
                     /locus_tag="JD016_06190"
     CDS             <1..1085
                     /locus_tag="JD016_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAU4999224.1"
                     /translation="YPNKKQQELINKTFGCCRFVYNKYLAKRIDVYKNDKETFTYKQC
                     SSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKFKSKKINRFSYKT
                     NFTNGNIMYCGQYIKLPKLGMVKIRDKQVPQGRILNATISKEPSGRYYVSLCCTDVDI
                     EVFENTNNHIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQRELSRKTIGSLNR
                     NKARLKVAGLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISD
                     VSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCN
                     ETHDRDINASINILKEGLRLITIQNK"
     gene            1203..1508
                     /locus_tag="JD016_06195"
                     /pseudo
     CDS             1203..1508
                     /locus_tag="JD016_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            1521..2426
                     /gene="truB"
                     /locus_tag="JD016_06200"
     CDS             1521..2426
                     /gene="truB"
                     /locus_tag="JD016_06200"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAU4999225.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            2557..3486
                     /locus_tag="JD016_06205"
     CDS             2557..3486
                     /locus_tag="JD016_06205"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAU4999226.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            3486..3584
                     /locus_tag="JD016_06210"
     CDS             3486..3584
                     /locus_tag="JD016_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999227.1"
                     /translation="MYDKMIKENNHYSAFEYSNQCLVIGVSKKIRR"
     gene            3590..3847
                     /gene="rpsO"
                     /locus_tag="JD016_06215"
     CDS             3590..3847
                     /gene="rpsO"
                     /locus_tag="JD016_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAU4999228.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            4087..4932
                     /locus_tag="JD016_06220"
     CDS             4087..4932
                     /locus_tag="JD016_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAU4999229.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            5057..7168
                     /gene="pnp"
                     /locus_tag="JD016_06225"
     CDS             5057..7168
                     /gene="pnp"
                     /locus_tag="JD016_06225"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAU4999230.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            7450..8193
                     /locus_tag="JD016_06230"
     CDS             7450..8193
                     /locus_tag="JD016_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAU4999231.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            8351..9598
                     /locus_tag="JD016_06235"
     CDS             8351..9598
                     /locus_tag="JD016_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAU4999232.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            9633..9878
                     /locus_tag="JD016_06240"
     CDS             9633..9878
                     /locus_tag="JD016_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HAU4999233.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            9888..11111
                     /gene="dapG"
                     /locus_tag="JD016_06245"
     CDS             9888..11111
                     /gene="dapG"
                     /locus_tag="JD016_06245"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAU4999234.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            11224..11958
                     /locus_tag="JD016_06250"
     CDS             11224..11958
                     /locus_tag="JD016_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease"
                     /protein_id="HAU4999235.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            12081..14492
                     /locus_tag="JD016_06255"
     CDS             12081..14492
                     /locus_tag="JD016_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAU4999236.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            14495..15553
                     /gene="mnmH"
                     /locus_tag="JD016_06260"
     CDS             14495..15553
                     /gene="mnmH"
                     /locus_tag="JD016_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAU4999237.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            15547..16881
                     /gene="rimO"
                     /locus_tag="JD016_06265"
     CDS             15547..16881
                     /gene="rimO"
                     /locus_tag="JD016_06265"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAU4999238.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            16868..17410
                     /gene="pgsA"
                     /locus_tag="JD016_06270"
     CDS             16868..17410
                     /gene="pgsA"
                     /locus_tag="JD016_06270"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAU4999239.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            17698..18744
                     /gene="recA"
                     /locus_tag="JD016_06275"
     CDS             17698..18744
                     /gene="recA"
                     /locus_tag="JD016_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAU4999240.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            18920..20461
                     /gene="rny"
                     /locus_tag="JD016_06280"
     CDS             18920..20461
                     /gene="rny"
                     /locus_tag="JD016_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAU4999241.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            20775..23111
                     /locus_tag="JD016_06285"
     CDS             20775..23111
                     /locus_tag="JD016_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAU4999242.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMVLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
BASE COUNT         9438 a         2378 c         4381 g         7023 t
ORIGIN      
        1 tgtatccaaa taaaaagcaa caagagttaa ttaacaaaac ttttggatgt tgtagatttg
       61 tatataataa gtatcttgct aaaagaatag atgtttataa aaacgataaa gaaactttta
      121 cttataaaca gtgtagttct gatttgacca atttaaaaaa agaattaaaa tggcttaagg
      181 aacctgacaa attttcacta caaaacgcct taaaagattt agataatgca tataaaaaat
      241 tctttaaaga aaaggcagga tttcctaaat ttaaatcaaa gaaaattaat agattttcat
      301 ataaaactaa ctttacaaat ggaaacatta tgtattgtgg tcaatatata aaactaccta
      361 aacttggtat ggttaagata agggataaac aagtacctca aggtagaata cttaatgcta
      421 ctatatctaa agaaccaagt ggtagatatt atgtatcatt atgctgtact gatgtagata
      481 ttgaagtatt tgaaaatact aataatcata taggtttaga tttaggaatt aaagaatttt
      541 gtatctcaag ttgtggagac tttatagaaa atcctaagta tcttaagaag tcattaaaca
      601 agcttgctaa attacaaaga gaattatcga gaaaaacaat cggtagttta aatagaaata
      661 aagcaagatt aaaagttgca ggacttcaag aacatatagc taatcaaaga aatgattttc
      721 tacaaaaact atctactaaa ttaataaaag agaatgatat tatttgcata gaagatttac
      781 aagtaaagaa tatgattaga aatcgtaaac tatctcgctt aatttcagat gtatcatggt
      841 cagaatttat tcgtcaatta gaatacaagg ctaattggta tggtagaaaa attgtaaagg
      901 taggtaaatt ctttgcaagt tcacaaatat gcaataaatg tgggtataaa aacgaggaag
      961 ttaaggattt aaatataaga gaatggattt gtcctagttg taatgaaact cacgatagag
     1021 atataaatgc aagtataaat atattaaaag aaggattaag actaataacg attcaaaata
     1081 aataacttat gtaagaaccg taggaactac ggggatagcc tgtggagaat tagtaagaca
     1141 tattttaata tgcaaaattc tgtgaagcag gaaccctgtg actttagtca tgggaggttc
     1201 agtcaaaggt atctagtata gttttaactc aagacatgat gaatagaaat aatattagct
     1261 ttaatgatgt tgatggaata acaacatata caagagacat aaaaggtata gaagttggaa
     1321 ttttatttaa agagaaaaaa caaaatgaaa ttaaagtgag ttttaggtca aaaaattatg
     1381 ttgatgtaag tgaaatagcc aagttatttg gtggtggtgg tcatgtaagg gctgctggat
     1441 gtactattag agatagtatt gataatgcaa agaaaatggt actagaggca gtgttaaaat
     1501 caatttaagg tgacagtaaa atgaataaga ttataagtat attgaaaccc acaggtatga
     1561 catctcatga tgttgtaagt agagtgagaa agattttaaa tataaaaaaa gttggtcata
     1621 caggtactct tgacccagat gcttcaggtg ttttaccaat ctgtataggg aaagctacaa
     1681 aagtatgtga agttatttta aacaaagata aatcgtatat atgtgagctt actttaggta
     1741 taagtacaga tacttatgat gcttcaggtg aaattttaaa aaaagttgat gattttaaat
     1801 ttagtaatga agacatagaa agagcttttg acacacaaag aggagagata aatcaacttc
     1861 ctccaattta ttcagcttta aaggtaaatg gaaagagaat gtgtgattta gtaagaagtg
     1921 gtagacaatc tgaaattact ttaaaaacaa gaagagtaaa tataaaagac ataaaaatac
     1981 taagtataaa aggtaataaa gtaatgtttt atgttgagtg ttctaaaggg acttatgtga
     2041 gaagtatttg ccatgatata ggagaatatc taggttgtgg agctcatatg tcgtttttga
     2101 atagaacatc ttctggtaaa tttgacttag ataattcaat aacattagaa gaattagaat
     2161 tattttatga aaataaaact ttagacaaat acttatatga tatagattat gtccttgatt
     2221 cgtttaatta tgttgtatta aaccctaatg ctataaaata ttatagtaat ggtggctcaa
     2281 tagatgataa aaggttttta aaaaataatt tcgataaaga tgatgaattt gtaagagtat
     2341 acagtacaga taatttctta ggattaggaa aattatcaaa acacaataat acaataagtg
     2401 ttaaaagtga taagatgttc atataaccaa tgtatttaaa aaaacaacaa aagagatagt
     2461 actataataa cttctatagt ctatctcttt tacttatgct ataatagttt gtgaataagt
     2521 taagagtaaa ttatatttta gcggggaaca tttattatgg tgattataaa atctataaaa
     2581 gaaatagcaa acataaaaga aagtgtcatt accataggaa attttgatgg tgtacacaaa
     2641 ggacatcaag ttcttatagg aaaaacagtt gaacatgcaa aaaaagaaaa tatacagagt
     2701 atcgtattta catttgcaaa tcatcctgta aattacttta gaccaaactc aacaaaaaat
     2761 attataagca atgatgataa aataaaatta ttagaagatt tgggaataga tatagttgta
     2821 atcataccat ttgatgagta tatgactaaa atacctgcaa aggatttcgt agatgagata
     2881 ctagtaaaaa aattaaaagc taaaaagata gtaattggac atgactttac atttgcaagg
     2941 gcaaaagaag gcaatgttaa tctattaaaa agcttagaac atgaatttgg ttttgaggta
     3001 gaagtaataa aacccatcaa aatcaatgat gtaagagtga gtagtactta tattagaagt
     3061 ttagtatccc agggtgatat ggctaatgtc aaggaatatt tgggtagaaa ttacaagtta
     3121 gaaggttgtg ttatacattc taaacacctt ggaagaacca taggattccc aacggcaaat
     3181 attgacctta agaataatat gctcgttcca aagagaggta tttatgcttc tattgtacat
     3241 attggagatg agacttattt tggtgctaca aatgtaggct ataacccaac agttaatgga
     3301 aaaactttgt ctattgaaac aaatatttta gagtttgata gagatattta tggggaaaat
     3361 ataattgttg aatttttgga aagaataaga gatgaaagaa agtttaattc aatagatgac
     3421 ttaaaaaatc aattgtataa tgatacaaat tatgtgtatg aaaactatgt ttgcaaaaac
     3481 aagtaatgta tgataaaatg attaaggaaa ataaccatta ctcggcattt gaatactcca
     3541 atcaatgctt agtaataggc gtttctaaaa aaatacggag gtaatctaaa tgataaacaa
     3601 atcagaaata ataaaagaat atggaagagc tgaaggggat actggttctc cagaagtaca
     3661 aatagcttta ttaacagcta gaataaacga gttaaatgga catttaaaaa ctcataagaa
     3721 agaccaccat tcaagaagag gactattaaa aatggttggt agaagaagaa acttattagc
     3781 ttacttaaag gaaaaagatc ttgaaggata tagagcatta atagctaaat taggattaag
     3841 aaagtaattt cattctttat aaaaaagcag gtatagtgta tacctgcttt tttatagtta
     3901 attcattata aagtaaaata gttaataata aaagtagttt atatgttata aaaaattata
     3961 aaatgaataa tattgggtaa gaatattaaa atagtataca aatacataat caatttaaaa
     4021 ggaaagacag gataaatata gaaattttat aacaggataa ttagttggaa taaggaggat
     4081 attattttga aaataaagtt agtatgcgat agtttgtgcg atattccaga tgaaatatca
     4141 gaaaaagatt atatagagat agttccactt acagttattt ttaatgatag ggaatatata
     4201 gaaggagtag atataaagaa agaagagttt tataaaaaag taaaagaaat caagcaaata
     4261 cctaaaactt cacaagctac atatatggag tttaaagaag tgtttgataa gttcataaca
     4321 gaaggttatc atataatctg tatgacaggg gctgcaaacg catcaggaac ttttcaaagt
     4381 gctatgatag ctaaaaatga tgtaaatgaa aatgaaaaaa tacatatatt tgatactaga
     4441 aatttatctc taggtagtgg acaatatgtt ataaaggcat gtgaacttct tgaagaagga
     4501 ctgggatttg aagaaattat agatgaatta gaaaatactc gcagtagtgt aagattgctt
     4561 tttgcaccat atacacttga ttttttaaag cagagtggaa gagttccagt tgcaactgct
     4621 ttgataggaa atatgttaaa tataaagcct atatttttct ttgataatgg ggaagctaag
     4681 ttggtaaata aggttagagg aataaagaat atagcaagta aattggtcga tataatttta
     4741 gaaatgaatg aagggtcttt agaaggaaaa atcgtcacaa taggttgtgg agataattta
     4801 cacgattgtg aaatattaaa agatgaagta aataaaagaa taaaagcaag aagagtatta
     4861 tttactcgag gaggagtttc tatttgctct catacagggc cagatatatt ggctataagt
     4921 tgttctaatt agtagtaata taaggttttt gcatatatat gtgaagataa ttttatattg
     4981 ttaatttata gtatttttga taatatagta aatagagtga tatacgtgtt ttttaataag
     5041 aggaggacaa gcattaatgt ttgaacataa aatatttaaa atggattttg caggaagaga
     5101 actttctgta gaaataggaa agatttgtga aatggctagt ggtagttgta tagttcgata
     5161 cagtgatagt atggtaatgg taaatacaac taaatcagcc aaacctagag acggaataga
     5221 cttttttcct ttaagtgtgg attatgaaga aaaattatac tctgtaggta agatacctgg
     5281 aggtttttta aagagagaag gcaagccttc agagaaagct atacttacat caagattgat
     5341 agatagacca ataagacctt tattcccaaa aggatttaga aatgatgttc aggtagttgc
     5401 aacagttcta tcagtagacc aagattgtac accagatata gtagcaatga taggttcttc
     5461 aatagccctt tctatatcag acataccatt taatggacca acaggttcag tatgtgttgg
     5521 ccttgtagat ggtgcatttg ttgtaaatcc aaacgcagag caaagagaaa aaagttcaat
     5581 gcatctagtt gtttcaggta caaaggaagc aataatgatg gttgaagccg gagctgatga
     5641 agttccagac gaggtaatgc ttgatgctat tttatttgct caccaagaaa taaaaaaaat
     5701 agttgagttt atagaaggta tagttgcaga agttggaaaa gaaaaaatgc ctgttgaatt
     5761 ataccatgca ggagaagaaa taactcaatt ggttcgcgaa tttgcaactg ataagatgaa
     5821 aaaagcagtt cagacttttg aaaagttaga gagaatggaa aatatggata gagttaaaga
     5881 agaaacttta gctcactttg aagaaacttt agaagatttt gaagattttg ttggagatat
     5941 agaagaagta ttacaagata taataaaaga agaagtaaga aaacttatag tacatgaaaa
     6001 tgtaagacca gataatagaa aacttgaaga aataagacct atatggtgtg aaactggcat
     6061 gatacctaga gctcatggtt cagccatatt tactagagga caaactcaag tacttaatgt
     6121 tgctacttta ggtgctttag gagatgttca aaaactagat gggttagatg aagaagaaaa
     6181 caagagatat atgcatcatt ataatttccc tgcatatagt gttggagaag ctagaccatc
     6241 aagaggacca ggaagaagag aaataggaca tggtgcttta gcagaaagag ctttattacc
     6301 agtaatacca tctcaagaag aatttcctta tgctattaga ttagtttctg aagtattaag
     6361 ttcaaatggt tctacatctc aagcaagtgt atgtggctca acattatctt tacttgatgc
     6421 aggtgtacct ataaaagata tggttgcagg tatagcaatg ggacttataa agcatgatgg
     6481 taaggttgca gtgctttcag atatacaagg tatggaagac catttaggcg atatggactt
     6541 taaagtagct ggaacagagt atggtataac agcaatacaa atggatataa aaattgatgg
     6601 tatagataaa gaaattctac aacgtgcttt aaaacaagct aaagagggta gaatccatat
     6661 actaggagag atgagaaaaa ctatatctca gccaaaacca gaactttcac catatgcacc
     6721 taagattgtt aaaatgcaaa taaatcctga caagataaaa gatgtaatag gacctggtgg
     6781 aaagataata actaagataa tagacgaaac tggagttaag atagatattg aacaaactgg
     6841 ggaagtattt atatcaggta ttgagattga tatgattaag aaagctcaag aacttataaa
     6901 taatatagta gttgaaccag aagtaggaaa aacttataaa ggtaaggtaa gtagaataat
     6961 gaactttgga gcatttgttg agatacttcc aggaaaagaa ggattacttc atatatctca
     7021 tatagctcat gaaagagttg ccaaagttga agatgtattg aatatagggg atgaagttga
     7081 agttaaagtt acagagattg atgaaaaagg aagagttaat ttatcaagaa aagttctttt
     7141 accaaaacca gaacataaaa ataaataact tattatataa ataacttatt atataattaa
     7201 aagcaaagtc ttaggatttt gcttttttaa atacaaaaaa gtagataatt ttaaaatgat
     7261 agttatggat ggcttatagt tctttttctg tcaggcttta ctcttataaa gaagctattt
     7321 aaaagctgat taatagagag ctttattgat atatatagaa tcacttaagt tatatttagt
     7381 gcataaacag agcttatttt taatagtatt aaataagaaa caatttgtac ataattaggg
     7441 ggagttttaa tgataatgat ggtacttaat aagagtaagc tcaaaaaaat cataatacta
     7501 attttagtag tgttattagt attatcagga ggggtaatgg tattaaagtt atttaacaac
     7561 aagccagtat ttaatttatt taatggtgta gatttaccat atgaaagagg tacaaaggat
     7621 aaaagtggat atgttgcatt aacctgtaat atagaccttg gatgggaaac agagtacgta
     7681 gaatccattt tagaaacact taaaaaagaa aatgtaaaga ttacatttaa tgtaactgga
     7741 aaatgggcag aaaagaataa ggatgaatta ttaaagataa aaaaacaagg acatgaaata
     7801 ggtaatcatg gatataaaca tttagattat tctacattat catatgaaga caattatgag
     7861 caaatagaga cctctaaaaa gataattgaa gaaattatag gtgaaaaaac taaatttttt
     7921 caagcaccag caggttcttt tggtccagaa acagtaaagg ctgctaaagc attagggtat
     7981 acttctatca aatgggatgc cgatactata gattggaaat acaaagacca accagaggta
     8041 atcattgata gaatgaagaa aaaagacatt aaagatagta gtataatttt aatgcatcca
     8101 accaacgcaa cgacaaaatg tatagatgat attatagcta ttgtaagaga aaaaggacta
     8161 aaaccaggaa aacttagtga tgtatttaag tagacaaagt ataaatattt ggttaagatt
     8221 gtataagttg tagttgaatt aattaaaaga ctgacttata atgtaataca tataaaaaat
     8281 aaatataatt aaaacatagt gttttcaata atccaatatg aaacaaagaa taaattgtta
     8341 ggagaatgta atgtataaga caaaaatact ggaaaatggg cttacaataa ttggtgaaga
     8401 aataccatat ctaaagtcaa taacattagg tatttggata aatgcaggct ctaggataga
     8461 agaagcacag gtgagtggaa cttctcattt tatagagcat atgatgttta aaggaaccaa
     8521 aaacagaaca tcaaaagaga ttgctagttc aatagataat ttgggtggtc aaataaatgc
     8581 atttacaagt aaagaatgta catgttatta tgtaaaacta atagatgagc atatagatac
     8641 aggaattgat gtattgagtg atatgatact aaattctaaa tttgataaaa atgatataga
     8701 taaggaaaga cttataatat tggaagaact taagatgtat gaagactctc cagatgactt
     8761 atcttatgat ttattagtgg aaaatatata tgctaatgat ggattaggaa tgaatataat
     8821 tgggacaaaa gaatctctgt acaatataac tagagaatcc atgttagaat acttaaataa
     8881 gtattatatt ccaaataacg ctgttatttc tatagctgga aactttaact ttgatgatat
     8941 ggtggagaaa atcaaatcaa aatttggaca ctgggaaaag aaaaatttaa gtatagacat
     9001 aagtgaggct aagtttaatc cttgctttat atcaaaaaat aaagatactg aacaggtgaa
     9061 cttagctatg tgcctaaaag gtattccttt tgaaaatgat gaagaagtct attcaatggc
     9121 agtggtaaat aatattttcg gtggaagtat aagctcaagg ctttttcaga aaataaggga
     9181 agaaaaaggt ttagtatatt caatatactc atcacagaca ttatatagga aatgtggtga
     9241 attaggtata tttgcaagca tgagtacaga aaatttacaa gatgtatata atttaataaa
     9301 gaaagaaatt gaaaatataa gagaaaacta tcttacagaa aaagaaatat ctgaaagcaa
     9361 ggaacagtta aaaggaaatt atatattgga tttagaaagt actagcagta gaatgatgtc
     9421 tactggtaaa tctatgttac ttagcaaaaa agtaaaaact acagatgaaa tacttgaatg
     9481 tataaataat gttaatatta attcaataaa gaaggttgtt gataaggtat tcaatatcga
     9541 aaatattggt acatgtatag ttggaagaga tgtagaaaag attctacact tggattaata
     9601 ttgaaaaaag taaatctctg gaggtataaa atatgaattt atctgaaata gcaggtaagg
     9661 aaatagttaa cttggtcaca ggagaaagat tgggggtcat tggagaatgt gaccttatac
     9721 tagatgaaac tacaggcggt atattagcgc ttctaattcc aaaagaaaaa ggattttttg
     9781 ccagaagaaa agataagtca tttttagaag taccatggag aaatgtaaaa aaaataggta
     9841 atgacatgat aattatagaa tatgagggat atatttagga ggaaaaaatg agtattttag
     9901 tgcaaaagtt tggtgggaca tcagtagaat catatgaaaa aatgaatgaa gtatgtaaga
     9961 tagtaaaagc atacaaaaaa aatgatgaag aattacaatt agtattagta gtttcagcaa
    10021 tgggaagaaa aggagctcca tatgctacag atacacttat aaatttgtgt agtgcagtaa
    10081 atgatgagcc aagtaaaaga gaacttgatt taataatgtc ttgtggagaa ataatatcag
    10141 gaacaattct tgctaattta ttgaatgcac aggggataga ttctgttttc tttactggtc
    10201 agcaagcagg tataattaca agtgatgaat attctaatgc aaagataaag tatataaatc
    10261 ctaaaaaggt aaaaagagct ttagatgatg gtaaggttgt agttatagct gggttccagg
    10321 gtgtaacaga tgatggagaa ataactactc ttggaagagg cggaagcgat acatcagcag
    10381 ttgctattgg taaagcttta gaatgtgaaa cagttgaaat ttacactgat gtggatggta
    10441 taatgactgc agaccctaga gtagaaccaa acgctaaagt attgagtttt atagactatg
    10501 aggaagtatt ccaaatggct gacaaaggtg caaaagtaat acatccaaga gcagtacagt
    10561 tggctaaaag tggaaatata actttagcca taaagaatac aatgaatcca acttttgaag
    10621 gtacaaaaat aggttcttta tgtagacact tagaagacaa tatagaatat gaacaagaaa
    10681 gagattttaa ggttgctgtt gcaaataaag atagcgtagc acaagttaag attaagtcag
    10741 cagaagaagt gtttacagaa gtattaaatg agatagaaaa gaaacttata actatagata
    10801 tgataaattt ctttatatca gaaaaagcat ttgttgtaga agatgctgat ataaaatcat
    10861 taaaggaaat actagataaa tttgaattag attatgaagt aaatagagac tgtgctaagg
    10921 taacattgat atgttcaaga attgctgatg aaatgtctgg tatcatgtca aaggtagtta
    10981 gaggattatc aaaagcaggg gtatctcttt tacaaacatc agactcaaat atgactatat
    11041 cttgtttagt tagtgaagaa gatatgcata cagcagtaca tgctatacac caacaatttt
    11101 atttaaaata aactgtataa tacaattatt ttttaatcaa atttaagaat ttcatatata
    11161 tcttaatata tttgatttat ttggtaaaac taaattaaat ataattttga taggagtttt
    11221 tttatgaaag attatagaga agataatgaa agatttgata aagataattg tagtgaagaa
    11281 tctagttgcg aagagagaga atgtataaag caatttggta ccaatgaaat gccacctcaa
    11341 cctcctaaag atattcaatg tataactata attggagaaa ttgagggaca ttttatagga
    11401 aatcctcaaa aaaaagctac aaagtacgag catataattc caatgctata ctcaattgag
    11461 gaaagtaatg atgttaaagg agtgttagtt gttttaaaca ctgttggagg agatattgaa
    11521 gcaggtttag ctattgcaga acttttaaac agtacatcta aaaaagtagt tactcttgtt
    11581 ctaggaggaa gtcatagtat aggtgttcct cttgctacag caggagatta ctcatttata
    11641 gcaccaacag caaccatgat tatacatcca gtgcgaacta ctggtcttgt gattggtata
    11701 aatgagactt ttgagtattt taaaaagatg caagatagaa taattcaatt tataataaga
    11761 acatcaaata tcaaaaaaga tgtacttgaa aaacttatgc atgaaaaaga tgaacttgta
    11821 agtgatgttg gtagtgtatt aataggaaaa gaagctgtgg attatggtct tatagatgaa
    11881 gttggaggac taaaagaagc acttaaaaaa ttaagagagc ttataaaaga atctgaggaa
    11941 gaaaaaaata atgattaaaa tttgtttaaa taataaaatt tatgatataa tgatgtaata
    12001 aagtaggagt gttagtgtta aggcattagc actttttgct gtgaaggggt aaaacttgct
    12061 aaaaataagg agtgatatta atggccacga aaaagaagaa aaaaaagaag gtaaatacct
    12121 taagttttaa tgcagaatat cataatttaa taacaatatt tataggtgta tttttattat
    12181 atagtttaaa ttctaattct atgggatgga tacctgtgtt aatgcaaaat ctttttaagg
    12241 gactatttgg agggctttca atagcaatac catttatagt aattataaca ggacttttag
    12301 gattttttga tggaaatgaa tatatatata ggctaagaaa gactaaacta tactacataa
    12361 ttattttatt tatatttgta ttttatggat tattaaatgc aggaactctt cctgtagata
    12421 gcccactaaa aggtaacatg tttgatgatg ttatgaaatt gggtgtaagt ggacaaggta
    12481 gtggtttgat agcaacaact attgcgtatt atatgagtaa aatatttggt atagcaggtg
    12541 gatggttaat aagtatcttc gcacttatac tttctgtaat gtttatattt aatatttcta
    12601 taaaagattt ggtgtcaaat gcaaaatcaa aatctaaagc atctaaagac agcaatttaa
    12661 cgtttaaaga taaaattgca aatatgaaaa aatctgcaat agatatgatg actgatgaag
    12721 tagatgatac tactataaat aaaaaaccag gtttctttaa agggttaatg tcaaaaggtc
    12781 ttaataatga tgatgaagaa gatgaatatt ttgaagaaga aaatagtgat ggtgtagatg
    12841 ataaaactat aaaaatagtt ggatttaata aggcagaaga tgagtattta gaaatattag
    12901 aaggaactca aagtatgcca gagttagatg ttttaaaaga attacaaaaa gctaccaatg
    12961 aaaatccagt tgtagatact aaaccagaaa aaaaagttga tatagctaaa cctaatttaa
    13021 atatagaaaa aactcaacct atgagtattg tagcagagcc agttaatgag gattattcaa
    13081 actataaaaa gcctagcatt gagcttttaa ataaagtaaa taagaagtct gatgaaaatg
    13141 gtaaaaagaa agtgttgaaa aatgcttctc ttttagaaaa gacactatca gattttggag
    13201 tggaagcaaa aattaatcaa gttacagttg gacctactat aacaagatat gaaatacaac
    13261 caagtccagg agttaaggta agtaaaattg taaatttaac tgatgatata gctttaagtt
    13321 tagcagctaa aagtataaga attgaagccc caattcctgg taaaagtgca attggaatag
    13381 aagttccaaa tgaagaagct caaatggttg gtgtgagaga agttctagaa agtgaagaat
    13441 ttaataattt taactctcct cttgcaatgg gacttggaaa agacgtagca ggaaaaatta
    13501 taattggaga tataggtaag atgccacact tactaatagc tggttctaca ggttcaggaa
    13561 agagtgtgtg tgtaaataca ttaataagta gtattttata taaggcaaat cctgatgaag
    13621 taaaattact attaatagac ccaaaagtag ttgaacttgc aaattataat ggaataccac
    13681 atctattgat accagtagtt actgacccta aaaaagctgc aaatgcttta aattgggcag
    13741 ttacagagat gaatagaagg tacaaattat ttgcagatgc tcaagtaaaa gatgtaacaa
    13801 gttataatga gaaagcggaa gaaaaacttc ctaaaatagt aataataata gatgagttag
    13861 cagatttaat gatggcaagt gcaaatgatg tagaagatta tatttgtaga ttagctcaaa
    13921 tggcaagagc tgctggaatg cacttgattg tagcaactca aagaccatct gttgacgtta
    13981 taacaggggt aataaaggct aatataccat ctagaatagc atttgcagta tcatctcaaa
    14041 ctgattcaag aacaatcctt gatatgggtg gtgcagagaa gcttctaggt aaaggtgata
    14101 tgttattcta tccacttggt gcagcaaaac cagttagact tcaaggggca ttcatatcag
    14161 agagtgagtc ggaaaaagta atcgattttg taaaaagtca ggttaaagat ggaataaaat
    14221 atgaagaaga tataattgaa actatatcta aggttaatac ttcaaagggt agtgatgagg
    14281 atgagttttt gagtgaagcg atagaatttg tcgtggaaag tggtcaagca tctgcctcta
    14341 tgctacagag aagatttaaa ataggattta atagagcagc tagacttata gattctatgg
    14401 aagaaagagg aataattgga gcaagtgagg gaagtaaacc gagaaaggta ttaataagca
    14461 aacaggattt gcagaattta gaaggtgaat agaattgtca gttataaaaa tagaaaaagc
    14521 acttaagcta gataaagtaa tatttgtaga tgtgagaact gaaggagaat atgaagaaga
    14581 ccatatattg aatgcaatca atatgcctct ttttaaaaat aatgaacata atgaagttgg
    14641 aacaatttat aagatgcagg gaaaacatga agcaatacaa aaagggtttg actatgtatc
    14701 gtataaatta aaggatatat atttacaagc agcagaactt gcattaaatt atgataatat
    14761 tgtcatatat tgtgcaagag gcggaatgag aagtggcagt atagtaaatt tattatcatc
    14821 attaggtgta aatgtatatc aacttgaagg tggatataag gcttatagaa attttgtttt
    14881 agagtattta aggcatgtaa tggatacaaa aaactttgta tctgttcatg gattgactgg
    14941 tacaggaaag actgatttgt tacatttatt agatgaaaaa ggaatagacg tgctggattt
    15001 agaaggcata gctaaaaaca gtggttctac ctttggtttt ataacttttg ataaaaagcc
    15061 accatctcaa aagaactttg aaacaaaact atttgaaagt atattttttt cgaaagagaa
    15121 ttatatattt gtcgagagtg aaagcaaacg agttggaagt gttacagttc caaatgaaat
    15181 tttcgaggca atgacacgag aaggacatca tatattatta gaatgtgata ttgaaaatag
    15241 agttgataga ctttgtagag attatattta tgataaagat gaaggtaata tattgatttt
    15301 aaaagaatgt ataaacaagt ttagaaaaag attgggtaat aaagtagtag atgattatat
    15361 tgatttatta gaatcaggtg aatataaaga acttgtaaaa agatatcttt tagaatacta
    15421 cgacccactt tatatgcatt cagtagagaa atataagtat agtaagatta taaattttaa
    15481 tgatacacaa aaagctttag aagatgttat agacttatat gagagtatat tggaaggaga
    15541 aagtgaatgt taaaaatagc attggaatca ttaggatgct caaaaaattt agtagatgca
    15601 gaaataatga tgggaatttt aaataacaaa ggctataaat taattggaga ttttgaagaa
    15661 gctgatgtaa ttatagtaaa tacatgtggg tttatagagt cagctaaaca agaatctata
    15721 gatacgataa taaactttgc agagttaaaa aaaactggca atttaaagtt gttgatagta
    15781 acagggtgtc tagcccaaag atattctgaa gaattaaaaa ctgaaattcc tgaaatagat
    15841 gctatagtag gaacaggaag ctatcaaaat atagataaaa tacttaaaga attaagtgaa
    15901 atgcatcaaa ttgtaagcct taatgatata gagtttgtat ttaatgaaga tttaccaaga
    15961 tatatatcta cacctagcta tatggcctat ttaaaaattg gagaaggatg ctccaataat
    16021 tgtacttatt gtataatacc aaaactaaga ggtaaatata gaagtagaaa atttgaagat
    16081 ataattaaag aagctaagaa attagctgaa tctggagtaa aagaacttgt ggttatagca
    16141 caggatacga caaagtatgg ttttgattta tatggaaaag aaagattatc agaactttta
    16201 gaagaacttg caaaaataga tggatttaag tggataaggg ttatgtattc atatccagaa
    16261 tctataacag aagaattgat acaagttata aagaaatacg ataatatatg tagttacttt
    16321 gatatgccaa tacagcatgc aagtaacaat attttaaaat taatgaatag aaaaactact
    16381 aaagaagata tattaaataa aatcaatctg ataagaagta atataccaga tgctatactt
    16441 agaactacaa taatagtagg gtttcctggt gaaacagaag atgacttcaa acaacttgtg
    16501 gactttgttg aagaggttaa atttgataga cttggtgcat ttgcatattc aagagaagaa
    16561 gatacaccag cagatagatt gccaaaccat atagatgaag aagtaaaaat acaaagaaga
    16621 gatactttaa tgatgattca acaaaaaata tctgaagaac ttaatgacaa aaagatagga
    16681 aaaacttatg aggtgttaat agaagaacag atagaagata atgtatatac aggaagaaca
    16741 caaggtgatg cagaagaaat tgacagtata gtgtatgtaa aaagtgtaga caatcttgaa
    16801 gtaggagaat ttgtaagtgt tcaaataaat gatgcaatgg aatacgattt aatgggagat
    16861 gttttatatg aacttgccta acaagttaac tttgtttaga atatttttaa taccagtatt
    16921 tgttttaatt atgttgctca atgtaccaaa taaattttta atagcttgta ttatttttat
    16981 aatagcttca attactgatg ctttagatgg aaaaatagct agaaaataca atctagtcac
    17041 tgactttgga aaatttatgg acccattagc agataagtta ttggttattt cagcacttac
    17101 atgtatgata gaagaccatc ttgtaagtag ttggatggta attataatag tggctagaga
    17161 gcttacagtt agcatattaa gagctatagc tgctgctgat ggaaaagtaa ttgctgctgg
    17221 aaacagtgga aaacttaaaa cgataactca aatggtatct atagtgtttt tattgttagg
    17281 tgctcagttt gaaaatgtat tgatattaaa tattggtgaa attctcattc ttatagctac
    17341 actacttaca ttatattctg gatgggaata tctatataaa aataaagagc tttttatgtc
    17401 ttctaagtaa aaaattatgc tttaataaat aaaaactaat gattaataaa taaaaaataa
    17461 caacttaaaa ttttaaaaat gtaaaaaagg gtatactatt atagtatacc tcttttcatt
    17521 ttaagaatat tttcttttgt gtatataatt attaataatg gtttataaat aaaatgtaat
    17581 gaataaaaat ataatcatta gtagcgtttt taaattgact aggtttgatt tatgttatat
    17641 aattaatata taacaagaga acaaatgttt gtagataaat gaagggatgg tatagagatg
    17701 agtgtagatc aagaaaaatt aaaagcgttg aatgaagctt taggtaaaat tgaaaaagat
    17761 tttggtaaag gttcagtaat gaaattggga gaagcaacat ctatgtctat agatgttata
    17821 tcaacaggag cgattggttt agacatagct attggtatag gaggtctacc tagagggaga
    17881 atagttgaag tatatggtcc agaatcttct ggtaagacta ctgttgcgct tagttgtgta
    17941 gcatcagctc aaaaagatgg aggaatagct gcatttatag atgcagaaca tgcacttgac
    18001 ccagtatatg caaaagcttt gggtgtggat gttgataacc taataatatc tcaaccagat
    18061 acaggtgaac aggctttaga gatagcagag gcattgataa gaagtggagc gatagatata
    18121 atagtaatag actcagtagc agcattagtt ccaaaggctg aaatagatgg agatatgggt
    18181 gattctcatg taggtttaca agctagactt atgtcacaag cacttagaaa gttaactggt
    18241 tcaattaaaa aatcaaattg tgttgctata tttataaacc agttaagaga gaaagtagga
    18301 ataatgtttg gaaacccaga aactactact ggaggacgtg cactaaaatt ctattcatca
    18361 gttagattgg atgttagaaa aatagataca ataaaacaag gtgataaagt tataggtagt
    18421 agaactagag ttaaagttgt taaaaacaaa gtagcaccac catttaagca agctgaattt
    18481 gatataatgt atggagaagg gatttcaaaa attggagacc ttttagatat agctgctgat
    18541 gtagatatag ttaaaaaatc aggttcatgg tatagttaca atgatactaa acttggacaa
    18601 ggaagagaaa atgttaaaaa attcttggaa gataatttag atttaactac tgaaatagat
    18661 gagaaagtta gagcatttta caatttaaat gaagaacatg aagaatcagg tacttcagta
    18721 tcaaaagaaa ttgtagaaga ataagtaaaa atacaaaaaa ataactagta attaaagaca
    18781 tgccacgctc cctattagct tgacacaatg ttgaaaaaat tataaaatta agtgaggata
    18841 aaatatctaa atttaatttt aattattagg aatatttttt aatcatagtg atgacatttt
    18901 gggattatta acgaaggagg tggatgtcat agatagtgta gtaatgattg taataggtgc
    18961 agctgttggt attatagctg gatattttgt tagaaaaaat atatctgaag caaaaattgg
    19021 acaagctgaa aacttggcca aagaaataat agataaagca caccacgact cagaaacagt
    19081 acaaaaagaa aaattactag aagctaaaga agagatccat aagtggagaa cagaagctga
    19141 aagagaaaac agagaaagaa gaacagaagt acaaaagtat gaaaaaagag tagtacaaaa
    19201 agaagaagtc ttagatagaa aattacaaaa tttagaatct aaagaagtaa acctaagtga
    19261 aaaattaaag gtagtggaaa agaaagaaga agaagttgaa gtaataaaaa ctcaacagtt
    19321 agaaaaatta gaaagtatat caggaattac ttctgataag gccaaagaaa tcatcttaac
    19381 caatgcggag agagatgtaa gacgtgaaat gtctataatg ataaaagaga tagaaagtca
    19441 agctaaagaa gaagctgata agaaatcaag agaaattata ggatatgcaa ttcaaaaatg
    19501 tgcagcagat cacgtagccg aaactactgt aacagtagta agcttgccta atgatgaaat
    19561 gaaaggtaga ataataggta gggaaggtag aaatataagg actttagaaa ccttaacagg
    19621 tatagacctt ataatagatg acactccaga agccgtaatt ttatcaggat ttgatccaat
    19681 aagaagagaa attgcaagaa ttgctctaga aaaattaata gcagatggaa gaatacatcc
    19741 agctagaatt gaagaaatgg ttgaaaaggc gagaaaagaa gttgacaata ttataaaaga
    19801 atatggtgaa caagctgcat ttgaaactag tgtacatggg attcatccgg aattaataag
    19861 attattaggt agacttaact atagaactag ttatggacaa aatgtactta aacattctat
    19921 agaagttgct catatagctg gtataatggc tgctgaaata ggagcagata ttagattggc
    19981 taagagggca ggtttattac atgatatagg taaggctgta gaccatgaaa tggaaggtac
    20041 acatgttgaa ataggtatgg atttacttag acgttataaa gagtctaaag aagttataca
    20101 tgccatgagt actcatcatg gagattacga gcctcaaaca gtagaggctg tattagttac
    20161 agcagcagat gcaatatcag ctgctagacc tggtgcgaga agagaaactt tagaagcata
    20221 tataaagaga cttgaaaaat tagaagaaat agctaattca tatgaaggtg tagaaaaatc
    20281 atttgctata caagctggta gagaaataag aataatggta aaaccagaag ctataaatga
    20341 tgaagaaata catctattgg ctagagatat gactaagaaa atagaggatg aacttgaata
    20401 tcctggacaa ataaaagtga gcataataag agaaacaaga gctatagagt atgcgaagta
    20461 aaagaaataa tatctaaata ataagaaaat atttttagaa atttaaattt gccacaagat
    20521 atgataatat atcttgtggc ttttaaattg tttaagttta attttatata atgcaaaaat
    20581 aatctaagtg ctaaaagtgc tacttacaaa agattaataa aataaagatt tatatatgga
    20641 ttatttatat aattatccaa gtttgtttga tataatgaag ttgtaaaata aatatattac
    20701 tacttggaat gcttgtactt aaataagatg aaaatacaat gacgctaaag tgaggattag
    20761 agagagggta cactatgaaa aaaaatacac ttttacatac aaatattatg gtttgcatta
    20821 ttattactct tggttttgtg gctacctcta taattggata tcaatctaat accaatattt
    20881 ttaaaaatga tattgaacat gtatctacat tggctgctga aggtatttat tatcagatag
    20941 ataaactact ttcagaacct ataaatgtat ctcttacaat ggcaaatgac agtctattaa
    21001 aaaatttttt agatggtgag aaagaacatt taaatgatga agaatttatt tataaattac
    21061 aagattatct gaatgtgtat agaaataaat attcatatga ttctgttttt ttagtatcaa
    21121 ctaaaaccaa taattattat cattttaatg gacttgatag aaatcttagc gctaataact
    21181 cagagaatca atggtattat actttcttaa agaatgatga tgaatactca ttaaatgttg
    21241 ataatgatga agcaagtaac aatagtatta cagtctttgt aaattgtaag ataaaagatg
    21301 ataatggagc aactatggga attataggtg ttgggttaaa ggttaattca ttacaaatgt
    21361 tgcttaaagg atataatgac aaatttgatg ttgttgcacg cttaattgat gataaaggat
    21421 ttgtacaatt agcagtagat aaaaccggtc atgagaatat aaattttttt gaaaattcta
    21481 gtagcgactt gtctaattcg aaacaattaa tattaaacaa caaaaaggaa caaaagagtt
    21541 tttggtattc atctgaaaaa tcaaaaagtt atattgtatg tcagtacata cctagtttaa
    21601 aatggcatct tgttctagaa aatgatttta ctttaatgat taaacagctt catctgcaat
    21661 tttttaaagg cattgcagtt atagttttaa ttataatatt tgtacttttt actattacac
    21721 atgtgcttaa aaaatacaaa aaccaaataa taaagcttag tgtttcacag aaattggaat
    21781 atcaaaaatt gttgagcaaa tctactgaag gtctttatga aaatattttt gaaattgata
    21841 ttactaacaa taaagctggt tgtgaagatg agaataggta ttttcatgaa tttggattag
    21901 ggcttaatac cccatatgat gaagccttac atacaatagc taaaaaccaa atcaaagaag
    21961 aatatgcaca aggttattta gatacatttt tacctgaaaa tgtaattaaa cgttataata
    22021 gcggaattaa taatatcagc tatgattttt taattaaaaa agatgaagaa agttatcatt
    22081 ggattagaat taatgcacga attttctttt ggacatcaga tgaatctgtt cgtatgatat
    22141 cttatcgtaa aaatatagat gaagaaaaaa atagagaatt actacttctc gaacgttcac
    22201 aacgtgattc tcttacaggg ctttacaata aagctacaac agaggaattt attcgttcat
    22261 ttttaaaaaa tgaaagtaat caagatttag aacacgtttt tctaattttc gatattgata
    22321 attttaaaaa tgtaaatgat aaatatggtc atgtatttgg agactttgtt ataagtgaat
    22381 ttgccttaga attaaaatct caatttcgtg aggacgatat tgtgggacgt attggtggag
    22441 atgagtttgt agtattaata aaagacttta ataataataa ctatgtgctt atagacaaat
    22501 tagagcgttt ttgttctaag attaatcaga aaaaatttgt tgagcaagca gaattttcta
    22561 ttgcatgtag tattggtgta gcaatgtatc ctgaacatgg atatgtatac tctgaactat
    22621 atgagaaagc agaccaagca ctttactatg caaaaagcca tgggaaaaat tcttatcata
    22681 tattttcaga ggattctatt gatagtattt catttcatat agactctaga gatataaaag
    22741 tattattaga aatggctact gatggtgttg tcaaatttgc ttgtatggaa aacttaaaat
    22801 tactttattt caaccaaaaa atgcttgaat ttactggata ttcagctcaa actgttactt
    22861 ctataggttt tgatattttt tcgcctgtat tacaagacga cttacaaatg gtactagata
    22921 cattaagaga agcaatttct aaaagagaga cttttgaaat agtttttcgt ttttgtcata
    22981 cagatggtca tatttttcct gtaaggatgc aaggtaagtt tgtaagtgaa ttatatcaaa
    23041 atcgttatcc tgttttttat gcgatttata cagacatatc aagtatcaat actttactag
    23101 aaaataaata gtaaatacta cagtaaaatc taaaaatagg atataatagt attaatataa
    23161 gaagattata aataaaacct gctaatgaaa atagattatt tttagcaggt ttttaaattt
//
LOCUS       DACXMZ010000041        22786 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.041, whole genome shotgun sequence.
ACCESSION   DACXMZ010000041 DACXMZ010000000
VERSION     DACXMZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.041"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            200..664
                     /locus_tag="JD016_06290"
     CDS             200..664
                     /locus_tag="JD016_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAU4999243.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            671..1165
                     /locus_tag="JD016_06295"
     CDS             671..1165
                     /locus_tag="JD016_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAU4999244.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1181..2206
                     /locus_tag="JD016_06300"
     CDS             1181..2206
                     /locus_tag="JD016_06300"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HAU4999245.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2193..4640
                     /locus_tag="JD016_06305"
     CDS             2193..4640
                     /locus_tag="JD016_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAU4999246.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4820..6193
                     /gene="radA"
                     /locus_tag="JD016_06310"
     CDS             4820..6193
                     /gene="radA"
                     /locus_tag="JD016_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAU4999247.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6196..7266
                     /gene="disA"
                     /locus_tag="JD016_06315"
     CDS             6196..7266
                     /gene="disA"
                     /locus_tag="JD016_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HAU4999248.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7355..8449
                     /locus_tag="JD016_06320"
     CDS             7355..8449
                     /locus_tag="JD016_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAU4999249.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8578..10755
                     /locus_tag="JD016_06325"
     CDS             8578..10755
                     /locus_tag="JD016_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999250.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(10941..11786)
                     /locus_tag="JD016_06330"
     CDS             complement(10941..11786)
                     /locus_tag="JD016_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAU4999251.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            12003..13169
                     /locus_tag="JD016_06335"
     CDS             12003..13169
                     /locus_tag="JD016_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HAU4999252.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            13191..14837
                     /locus_tag="JD016_06340"
     CDS             13191..14837
                     /locus_tag="JD016_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAU4999253.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            14934..16307
                     /locus_tag="JD016_06345"
     CDS             14934..16307
                     /locus_tag="JD016_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAU4999254.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWAKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQDYIG
                     VIFSSAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            16752..17705
                     /locus_tag="JD016_06350"
     CDS             16752..17705
                     /locus_tag="JD016_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAU4999255.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            17737..18705
                     /locus_tag="JD016_06355"
     CDS             17737..18705
                     /locus_tag="JD016_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HAU4999256.1"
                     /translation="MYMSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSV
                     GQEASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSM
                     HIADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGIN
                     LSSIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYET
                     VQKAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLI
                     ENNLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            18730..19716
                     /locus_tag="JD016_06360"
     CDS             18730..19716
                     /locus_tag="JD016_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAU4999257.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            19729..20775
                     /locus_tag="JD016_06365"
     CDS             19729..20775
                     /locus_tag="JD016_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin dehydrogenase"
                     /protein_id="HAU4999258.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            20942..22672
                     /gene="lpdA"
                     /locus_tag="JD016_06370"
     CDS             20942..22672
                     /gene="lpdA"
                     /locus_tag="JD016_06370"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAU4999259.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSMPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
BASE COUNT         9234 a         2380 c         4313 g         6859 t
ORIGIN      
        1 aatcatgaag caaaaaatta gcatttggtt atcaataaat ataataaata aaaactcata
       61 attaatgaaa gaataaattc atatctaaat ttctataaaa aatttaaatt tatacaattt
      121 agggctttaa aaatttaaat aataggtgta aaataatagt caaagaaagt caaaaataaa
      181 aaggtgtgtg gtgatattaa tggcaactat gacagatata attgaaaaat ttattaaaga
      241 tttgatggaa gaagataata gcatacaaat tcaaagaaat gaactagcaa acttgtttag
      301 ttgtgcacca tcacaaataa attatgtctt aacaacaaga tttacaatag atagaggata
      361 ttacattgaa agtaaaaaag gtggcggagg atatgttcaa atagaaaaaa tacgaaaaag
      421 taaagatggg catattagag aactactaaa tgaaaaaata ggaagtcaga tatcctataa
      481 aaaagcaaag gaattattag aaggtttaaa agagtcggat ttgattaacg aaagagaatt
      541 aaaattaata ttatatgcaa tagatgataa atcattgtgt atgccaatat atgaattaaa
      601 agagaaggta aggtctaata ttttaaagaa tataataata ggattattta gtatagagga
      661 gtgataattt atgctatgtc aaaaatgcaa taagaataaa gcatctgtat attataataa
      721 gatagtcaat ggagaaaaaa cagagatgta tttatgtagt gagtgtgcaa aagagaatac
      781 agaaatgaat tttaacttag atatgccttt ttcaatgatg gatatatttt ctaatttagg
      841 ttttcaacct aaaaaagaac ttgaggaaaa gctagtttgt cctaaatgca atacaacata
      901 tagtgagttt aagaataatg ggagatttgg atgtagtgaa tgttataatg catttagtag
      961 tcaagtaaat ccaatgcttc aaaatataca tgggcatata gaacatacag gaaaagcacc
     1021 taaaaaatct ttttataaga taagtgtaga aaatgagatt aaagaactaa aagaagattt
     1081 agatagagct ataaaaaatg aagagtatga attagcagct caatttagag ataaaattaa
     1141 atatttaaaa ggttctattg actaagattg gagtgatatt atgaaagaga acatagtcat
     1201 gaaatcaaga gttaggctag ctagaaattt aaataactat ccatttccaa ataaattaga
     1261 taaagaatgt gccatggaaa taatagaaaa agtaaaaaat gcgtttatta attctaactt
     1321 agaacaaaaa gaagaatttg atttctataa aattgaagat ttagaccaat ctaaaaaaat
     1381 gcttatggtt gaagaacata taataagccc agatttagca gaaaatgata aaagtgcagt
     1441 tatagtaaaa aaagataaaa ctataagtat aatgataaat gaagaagacc atataaggat
     1501 acaaactata tgtgatgatt taaacttaga atatgcatat agtgtagcaa atgaaataga
     1561 tgatttgtta gaatcaagtt tagaatatgc ctttaacaca aagctaggtt atctaacatc
     1621 ttgtccaact aatacaggaa caggaatgag agcttctgta atgatgcatt taccagcatt
     1681 aagtcaacta ggctatatgg atgaacttta taaaatttca tcacaaatag gtatagcaat
     1741 tagagggata tatggagaac gaacagaagc attgggaaat atatatcaaa tatctaatca
     1801 acttacactt ggaagaacag agtctaatat tatagaaaat gtaagtggac ttacaaaaga
     1861 tgcaatttca aaagaaataa aagctagaga aatattgcaa aaaaaattag gtataaaact
     1921 agaagataaa atatttagat cgatagggac acttgaaaat tcaagagtaa tgagtagcgc
     1981 agaggcaatg agccatctat caaatataaa aatgggaata gaaatgaact atatagataa
     2041 actagattta agggctatag aacaacttat gataggtata caaccagcgc atcagtcaat
     2101 aatgtataag agtgatgatg ttgagaatag agatataaat agagcaacat atattagaga
     2161 aactttggaa aaattaagag gagggatgaa ttgtgaactt caatagattt acacaaagag
     2221 ctaaaaaggc aattgattta gcgtttgaat ctgctaaaag tctaggacac aatattgtcg
     2281 gaagtgaaca tatactttta ggtcttttaa gagaagaaga aggtatagct gctaaagttc
     2341 taagtaaagt tggatttaca gaagcttatt tagaaggcaa aatagttgat atggaaggta
     2401 agggagaaga aatctcagaa gatatagtat taagtccaag aagtaaacaa atacttgaat
     2461 tatcaggaat gtttgcaaat aaattaaaaa caaattatat tgggactgag catatattat
     2521 tagcaattat tcaagaaggt gaaggaatag ctaataagat tttaaattat gctggagtaa
     2581 atgataggac tttagcccaa ttaacaattg atatgatggg tattagtgat aaaaatcaat
     2641 acaaagcaga aaatagttac acgggcaacc agaatcaagc agaatctaaa gtacttgata
     2701 agtatggaag aaatcttaca ctatatgcaa aacagaataa aatagaccct gtaataggta
     2761 gagaaaaaga aatacaaaga gtaatacaaa tattaagtag aagaactaaa aacaatccag
     2821 tgttgatagg agacccaggt gtagggaaaa cagccatagc tgaaggtctg gctacaaata
     2881 tagccttagg aaatgttcca gaaacactaa aaaacaaaac tttatactct ttagagatgg
     2941 gttcattatt ggctggggct aagtatagag gagaatttga agaaagaatt aaagaagttg
     3001 tagatgaagt tgttaaaaat ggaaacataa tcttatttat agatgagatg cacacaataa
     3061 taggggctgg gtctactgga gaaggctcta tagatgcttc aaatatatta aagccagcct
     3121 tagcaagagg agaaatacaa gttataggtg caactacaat tgatgaatat agaaagcatg
     3181 ttgaaaaaga ttctgctcta gaaagaagat tccaaccagt tatggtagat gaaccaacta
     3241 aagaggactc aataaaaata ttagaaggat taagagataa atatgaagct catcataaag
     3301 ttaaaataac tgatgatgct ataaaggcag ctgtagaatt atcaactaga tatatatcag
     3361 acagatatct gccagataaa gctatagact tgattgatga agctgcatca aaagtaagat
     3421 taaaagaaaa tacacctcct tcagaaataa aaaaattaga actagaaata gaaaatatag
     3481 ataaagaaaa agaagaagca gtaagatgtc aagactttga gaaagcagcc aaaataagag
     3541 atgaacaagg tctacttaaa aaacaattag aagatgttag agaaagatgg aacaagtcat
     3601 ctaaacattc agatttagtt gatggagaag ttattgctga agtagtgggc ttatggacag
     3661 gaataccagt taataaaatt cttgaagaag aggctgatag acttttgaga cttgaggaaa
     3721 tactgcacaa tagagttata ggtcaagaac aagcagtaaa atctatttca aaggcaatca
     3781 gaaggtcaag agcaggtctt aaagacccta atagaccaat tggttcattt ttatttttag
     3841 gtccaacagg agtaggtaaa acagaattat ctaaggcatt agcagaagtg caatttggag
     3901 atgaaaatca aataattaga atagatatgt ctgaatatat ggaaaaacat gctgtgtcaa
     3961 gaatgatagg ttcacctcca ggttatgtag gtcatgatga aggtggtcaa ttaactgaaa
     4021 aagtaagaag aaatccatat tcagttatct tatttgatga gatagaaaaa gctcatccag
     4081 atgtatttaa tattctgtta caaattctag atgatggtag actaactgat tcaaaaggaa
     4141 gaactgtaga ttttaagaat actattgtaa taatgacatc aaatgttgga gcatctacaa
     4201 ttggtagaca aaaaacttta ggatttagta tagctaaagg agacgaagaa gaaaaatctc
     4261 aatatgaaaa aatgaaagaa aatataatgg gtgaattaaa acaaagattc agaccagagt
     4321 ttttaaacag aatagatgat ataattgttt tccactcact aaatgaaaat catatatcaa
     4381 aaatagtact tttaatggca gcgaaactac aagagagatt aaaagaaatg gatataaaat
     4441 tagaaatgag tgatgaggct gttaaattaa tttctaaatc tggatttgac ttagaatatg
     4501 gagcaagacc tcttaaaaga gctttacaaa aagagttgga agatgagcta tctgaagcaa
     4561 tcttaaaagg taatgtgaaa aagggaagta atgtagtagc aaaagtcaaa gatgaaaaaa
     4621 tagtttttga aactaaataa tttaatagag ttgcatttta aaatattgca aatataagtt
     4681 ttataatcta aacaaaattt taagggcaat gaatctataa taattcattg tccttaatta
     4741 ttttatttaa tctttacata ttgtctttca gatatgctaa aattatagta tagaataaaa
     4801 aattaaggat gataaaagta tggcaaaaat aaaaacaaaa tatgtatgtc aatcttgcgg
     4861 atatgaaaca gctaaatggc ttggtaaatg tccagaatgt acaaaatgga atacatttgt
     4921 agaagaaata gaccaaaaaa gcaccaaaaa agaggtcttt ataatagata aatcttcatc
     4981 taaaccagtc agtataaact caatagaaag taaagaagaa gagcggttta caacagatat
     5041 aaatgagtta gatagagtgc ttggaggagg tatagttaaa ggctctttag tgcttgttgg
     5101 tggtgaccct ggtataggta aatcaacact tttaatccaa gtatctagta atgttgccaa
     5161 tttaggaaaa actgttttat atataactgg agaagaatca gaatctcaaa taaaaatgag
     5221 agctaaaaga ttaggtatta actcagaaaa tttatatata tttgctgaaa ataatttaag
     5281 tataattgaa tcttatttag aaagtgtaaa tccagaatta ataattctag actcaattca
     5341 aactgtcttc agtccagaaa tatcctcggc accaggaact gtcagccaaa ttaaagaagg
     5401 tacttcaaaa ttcatgaaaa tttctaaaaa aatgggaatt tcaacgttca tagttgggca
     5461 tgttacaaaa gaaggttcac tagcaggacc aaaattatta gagcatatgg ttgatactgt
     5521 tctatatttt gaaggagaaa gatataatac ttatagatta gtaagagctg ttaaaaatcg
     5581 atttggttca actaatgaac ttggagtatt tgaaatgagg gatttaggat tagtagaact
     5641 tgataatcca tctaaaattt taatctctga aaaaccaaaa gatgtagcag gttcagtaat
     5701 aatatctact gtagaaggta cgagaccaat gttacttgaa ttgcaagcat tagtttctcc
     5761 tacaagtttt ggtataccaa aaagaacatc tacaggagtc gattacaata gagtaggtat
     5821 gttattagca gtattagaaa aacgtgttgg tttgcaaata caaaatcaag atgtctatat
     5881 aaacatagtt ggaggaataa agataaatga accctcaatt gatttaggga tagccatttc
     5941 tgtagcatct agttttagaa atataccgat agatgaagat atagctgtta caggagaagt
     6001 gggacttaca ggagaagtta gagcagtaag ctttatagaa aagagaatag ctgagtgtaa
     6061 aaaacttgga tttaaaaaga tagtagttcc tagaagtaat tatgatgttg taaaagagac
     6121 caaaggcata gaaatatggc cagtagataa cttaagacaa gctataaata tagtacttgg
     6181 gaggaatcaa taaatatgga gaattttcta gataataaaa atatgctata tgcattaaaa
     6241 atgatatctc ctggaactcc acttagatta ggtctaaaca atgtactaag agctaagact
     6301 ggtggattaa ttgtaattgc aacaaacgaa gatgtaatga aaatagtaga tggaggattt
     6361 gctataaatg cagaatattc accatcatat ctatatgaat tagctaaaat ggatggagct
     6421 atagttttaa gtggtgatgt aaagaaaata ttatttgcta atgcacaact tatacctgac
     6481 tattttatag aaacatcaga gacaggaaca agacatagaa cagcagaaag agtagcaaaa
     6541 caaactggtg ctatagtcat aggaatttca caaagaagaa atgttataac agtttataga
     6601 ggaaatgaga agtatgtagt cgaagatata tctaagatat ttactaaggc aaatcaggct
     6661 atacaaactc tggaaaaata taagacagta ttggaccaag ctgtaacaaa tttaaatgcc
     6721 ttagagttta atgatttggt aactatttat gatgttgcat tagtcatgca aaagatggaa
     6781 atggtaatga gagttacaag tataattgaa aaatatgtga tagaattggg tgatgaagga
     6841 actttagtaa gtatgcaatt agaagaatta atgggtacaa ccagaataga ccagaaatta
     6901 atattcaaag attataataa agaaaacaca gaaataaaag aacttatgaa aaaggtcaaa
     6961 aatttaaatt cagaagaact aatagaattg gtcaatatgg caaaactatt agggtatagt
     7021 ggtttttcag aaagtatgga tatgcctata aaaacaagag gttataggat tcttagcaaa
     7081 atacatagac taccaacagc aataatagaa aacttagtaa attattttga aaactttcaa
     7141 caaattttag atgcatctat tgaagaatta gatgaggttg aaggaatagg tgaaataaga
     7201 gcaacatata taaaaaatgg actcataaaa atgaaacaat tagtcttatt agatagacac
     7261 atatgatgaa ttttaaatga attttactac ttttttcttg aatataatat acactttggt
     7321 ctataataga taagtataac tagggggtga agcattgata aggaaagtaa caagaatatt
     7381 gtttatttta cttggattta caataggtat aacaacctat ttaacattaa tgaaagattt
     7441 tgaaatatta acatttggaa aagaaacata tggttatatc gcagctgtag tagcaggtat
     7501 aataatagcc attttaggat atcttataga accatgggtt gtaaataaag taaaagaaat
     7561 tgctaaaata gtggataaag aattgtcaaa atatcctcag actgatatat tgttagggtc
     7621 tatgggtctt atagtaggat ttgttattgc gtatttatta agtggattag taaatagaat
     7681 accaatagta ggtggaatac tatccttact attatattta ttcctaggat atcttggaat
     7741 gaaagttgcg cttaaaagta aaaatgattt atttaatatt ggaaaactag gaagattagc
     7801 aaatcctatt aaggataaag ataaggataa agaaaataaa aaagaagtaa aagccattcc
     7861 tccaaaagta ttagatacaa gtgtaataat tgatggaaga atagctgata tttgtaagac
     7921 tgggtttata gaaggaaaac ttattattcc tgcttttgtt ttggaggaac taagacatat
     7981 tgcagattct tcagatgatt taaaaagagt aagaggtaga agaggtctgg atatattaaa
     8041 tataattcag aaagaattaa atatagaagt tgaaataagt gagagagact ttgatgatat
     8101 agcagaagtt gatagtaaat tattaaagct tgctcaggtt ttaaatggaa aagttgttac
     8161 aaatgattat aacttaaata aagttgctca attccaaggt gttgaagtat taaatataaa
     8221 tgagttagct aatgctataa aaccagttgc tataccaggt gaagatatgg ttgttcaagt
     8281 ggttaaagaa ggaaaagagg ctcaacaagg tgttgcatat ctagatgatg gaactatgat
     8341 agttgttgat ggaggaagaa aatatatgaa tgagactata aaagtactcg ttacttctgt
     8401 tcttcaaact cctgcaggta gaatgatatt tgcaaaacct aaaaattaat atgttcaaag
     8461 aaacctataa tttgggctgt ttattttaaa tagatggtcc aaatttattt atttataacg
     8521 ataatgttta atgtattctt aaagatgggg atttaaatca gaaagcaggg agaatttatg
     8581 agaaaaaaga ttatatcttt cttagcaaca tttatcataa tattaacatc agcaagtcag
     8641 tatagttttg cagatgatat atcaacacgt ggaaaagtaa tatttataga tatgaataga
     8701 actagtatga gtaatatgtt aagaataaaa tcattaagag aagaactaga caatagaggg
     8761 tatattggct tgatgaatat aagaggcgat aaaggaagtg atgatagaag gtcatatgct
     8821 agtatgggtg ctggtggtag agctaatgtg gctaatgaag aagatattaa ttttgaaagc
     8881 tcgagtaagg atagaaatat agtttttgaa tcagctacag gaaaaagtgc aaaaggtata
     8941 aataatttaa ctataaataa gtctataaat gaaaatttaa actttggaga gtatggttct
     9001 gtattagggt cattaggaca aagtttatct gataatggat taaaagcatc tgtattgggt
     9061 aattcagata taattgaaaa tggtcaactt ataaaaaata gaaacttatg tctaactgca
     9121 atggatgaat atggaagaat acctaatgga aatgtagata ctataaataa aaaagactta
     9181 tctatgcctt atggaatatc aactgattat gataaattga ttgtagagac taaagaagct
     9241 tacaaaaata atgatgtgat ttttgtggag cttggagaca catatagatt agatttatat
     9301 aaaccaaatt taaatgaaaa aacatatgag agtatgaaag ataacataga aaaaaatata
     9361 gatatatatt taaaaaatat attttctatg gttgaagaaa atgacactgt ctacatagca
     9421 agtgcatttc caagtgattt agattataaa aataaaagaa gactttcgcc aataataaaa
     9481 ttaaatggag aagggaaagg tatattatca tcttcaacta ctagaagaga aggtattgta
     9541 acaaatttag atgttggggt tgaaatatta gacaacttta atataaaaaa tcaaaatatg
     9601 gttggaagaa aatatgaact tattaacaga gatgataata aagaattttt gatggatgaa
     9661 tatcaaaaga tagtatctat ttcaagcata aggtctacaa tactgaatgg ttttgtaagt
     9721 atagttttct tgtcatggat agttgctatg attgcaattt tatttagaaa gcatatatca
     9781 aaaaattata aagaaacaac attttttata ttaaaggaac ttttaaaaat aggaatagta
     9841 atgccactgt catttatgat aacaccaata atgaatttca aaacaccatt agcaatatct
     9901 ttaggaataa ttataataac acttactata tatcttataa gtaaagtttt aataaagaac
     9961 gatttaaaaa acatgctgtt ttttactgga ttaaccatag taattatggt tattgatgct
    10021 gggtttggct catatttaat gaaaagcaat gttatgagtt atgattgtat aattggagct
    10081 agatattatg gagtaggaaa tgagtatcaa ggtgtggcaa tcggtagtgc aatatttaca
    10141 tttgcaattt tacttacata taaaaatata ccaaagtggt ctataatagt tttttctttg
    10201 gttatcttaa ttacaagtgc atctccaatt atgggagcaa atgttggaag tgctatttct
    10261 gaatgtgttg catttttact atttatacta ttgatatata atgtaaagat agatttcaaa
    10321 aaaatcgtat tattaggtat agcagtagta tttgtattag gagtttttgt agcaatagat
    10381 atgattttgg gaagtaattc acatttaggc atgtttgtaa aagaaattta ctttaatggg
    10441 ccaggagaaa taatacaaac atttagtaga aagatagaga tgaatttaaa gctagctcaa
    10501 accagtgcat gggtaaatat actgttaact ggaataggtg taattttagt tcttatgatt
    10561 aatcagatta gatactttaa gcaactgatg gatgagtatc caattgtatt taaaggattt
    10621 atagcatcaa tagcaggatg tttagtaact cttttagtga atgactctgg gattgtgtca
    10681 tcagctacag cctttattta tgtaattgta cctatgataa cattaagtat aaatcttaca
    10741 gcactaaaag aataagtact gtatgaataa tatgtaaata ttaatacatt aatttaactt
    10801 ctttatttac aaaataaaga agaaagttta taaaaaataa attataataa aaaagagttt
    10861 atctcaaaat tgattattct aatcaaattt taagataaac tttttttatt ataaagataa
    10921 ataacatata acctcatatt ttaggtagat ttttttctta tatgtttagg agtgcattta
    10981 aatttttcct taaaaattct gtaaaataat tttgtattag taaatccatg agcttctaat
    11041 atactgcata aggtatcatt tgtgttaatt aaatctttat aaatatgaga aatacgcaca
    11101 tcatttaaat actctaagta agtttgtccc atatactgtt taaattttct acaaaaatat
    11161 tctggttgta agtgtgctac cttagcgatt ttgttaatag aaataggcat tttgtaatta
    11221 atctttgtat attctaaaac tggttcaaat tttgacaaat caagtactgt ttttttcata
    11281 tttttattac ctacagatat tttaaagttt gtatataatt caaataaaag ttcaaataat
    11341 aagcttgtaa agagcagatt agctgcagga ggagaactaa gtttaatttc tctcatgttt
    11401 tctaatataa gtttaacttt acttgaattt tttttatagt tattgtcatt ggcattaacc
    11461 ttaaagtcaa aatagcaatt tttaaaatca gggatatatt tgtttagaaa agacaatggt
    11521 acttgcaata atatagaggt attttgataa atacatctag ttgagtgaat aattccagaa
    11581 ttaatgaaca tacactcatt agcttttaaa ttataagtgt aaccctccat gttaatttgt
    11641 aaatcaccag aagttatata aattatttct aatgagttat gccaatgatt tgctacataa
    11701 ctaccactat catcaaatag tttgaaatta acatctaaat tatcaatagg ttcaattatt
    11761 tcatgtgaaa aattatttct gttcataata atatcctcct tataatttta aattataaat
    11821 agtaaattta taatgttcaa tattaaaaag ataatataga ctaataaatt agataataaa
    11881 catacttaag aaaacggata aaatcaaata taataactgt atattaaaat aacttaaaaa
    11941 aattatttta ttataagtgc aaaatatacc tatttaaata agataagagg ggaggatttt
    12001 atgtgtatat aaaaggtgta aatttaggag gttggttagt tttagaaaaa tggatgacct
    12061 caagtttatt tgaaggcaca gaagctgaag atgaatacta tttaccaaga cagctttcaa
    12121 gagaggcata tgaatctaga attaagactc atcgtagtga atacatcaca gagagagatt
    12181 ttgcaacgat taaatctatg ggatttaatt cagttcgtat accagtacca tactttattt
    12241 ttggagattg tgaaccattc ataggatgtg ttaaagaact tgataaagca tttgcttggg
    12301 cagataagta tgggcttagt atattgatag acttacatac agttccagga agccaaaatg
    12361 gatttgataa tggaggaata tcaggtatat gtagctggtc acaaaatcca gaatatgtag
    12421 cgtttacatt aaatgtatta gaaagacttg caaaacgtta tgggatgaga catgaattgt
    12481 atggaatcca aattttaaat gagcctataa cagaaagaat gtggaatata atgaatgttc
    12541 caaatcggtt taaggctgta gataaggaga tggcaagggg gagtaaacct aattcattag
    12601 agtttttacg tgatttttat ataaaagcat ataaggtaat gcgtccttat atgagagagg
    12661 aaaacgttat agtgtttcat gatgcatttg aattaaaagc atggaaagac tttatgagag
    12721 aggaagaatt taaaaatgtt gttctagata cacaccaata tttgatgtta gctgaagcag
    12781 atggatgtga acaaagtatt gatagctatc tcaaatatat tagagaaaat tatgctaaag
    12841 atattttaca aatgcaaaaa tattttcctg ttatatgtgg ggagtggagc ttatttaatt
    12901 catatgcttg tggtatagat acaaatggag gtcaatcacc tctaaatggg attgaatcaa
    12961 atatagataa attatcaaaa gacgataaaa gagaattata taggaagata gcaaaagcac
    13021 agcttgatgc atggagaaat ggaagtggtc actactattg gaattataag ttattactag
    13081 atacagttaa tgaagaaggt tggataggat gggatagttg ggacctaggc aaatgtgtag
    13141 ctcaagaatg gtaccctata gaatattaaa ctaaaattgg aggaaaagat atgattaaaa
    13201 accctatact tccaggtttt aatccagatc cttgtatttg tagaaaggga gatgattatt
    13261 atcttgtagt ttcatctttt gaatggtttc caggtatacc tgtttatcac tctaaagact
    13321 taaaaaattg ggagttatat actcatattt tgacagatga aacaaaaata gatttgaaga
    13381 agcttccttc atctaaagga atttgggcac cctgtttgac ttattgtgaa gaagaagatt
    13441 tattttatat agtttatgga attatgaact ctatgaatgc aagatatttt gatgttgata
    13501 attatttaat tacatcaaaa gatataaagg gagagtggag tgaaccagta tatctacact
    13561 catcaggttt tgatgcgtct atttttcatg atgatgatgg taaaaagtgg attgcatcat
    13621 tagactggga gactagagaa ggatatgaaa aacctggtgt tatatgttta gtggaatact
    13681 gtacaaaaaa gaaagaaata gtaggatatc caaagagaat atggtcaggg ggaactgata
    13741 gaggttgtat tgaagcacca catataacaa agcgtggaga ttactattat ataatgtgtg
    13801 cagaaggtgg aactggatat ggtcatagtg taactatggg aagggctaaa aatatatggg
    13861 gaccttatga aaaagattca atgaatccaa ttgtaacttc aattccaggc gatttttatg
    13921 aaagacatga ccctgaccat ctaaagccta aatactataa tccagaatca aaacttcaaa
    13981 aatctggtca tggaagctat atagaaacaa catcaggtga agtatattta gttcatctaa
    14041 catctagacc atttgtacca gagttaaggt gtactttagg tagagaaaca gctattcaaa
    14101 aaatgaaatg gacaaaggat aattggcttc gaatggaaga tgaaagtaat ttggcaaaag
    14161 aatacgtatc agaaagtaaa ctagaagaac atttagtttc atctatacca agttttgatg
    14221 actttgattc aaatgaatta ggtttacagt actatgctcc taggatttca ccattatcat
    14281 ttgcagatgt taagtctaga ccaggatatg taaggattag aggacaagaa tcaagaacat
    14341 cattaaataa agtgagcata ttggcaagaa aattaacttc agtatatgca agaataacca
    14401 ctaagatgga gttttaccca gaggtacatc agcatagtgc gggtttgata atgtattatg
    14461 ataatatgaa ttatataaat ttaagaaagt attatagtga aacattgggt caaagtgcac
    14521 tatcaataat acatcttgaa aatggagaga agacggaatt tttaaatact aggataccaa
    14581 taaaagatat accaatatat cttcgtctgt atatacaagg tcgtaagtca tattttgaat
    14641 ggagttatga tgaaaaaaac taccagagaa taggaaaggt atttgataca actaagtttt
    14701 cagatgagta ctgtaaatat ggagaattta caggtacgtt tatagggtta acttgtgcag
    14761 atcgtgtaaa gcataaacac tatgctgatt ttgatttttt tgaatatata gtagatgaat
    14821 caaaagatgt cgattaataa aatcattact aaaattttca gagcaaaggt aaatcttatg
    14881 ctctgaaata agaaacatta gaaggcaaat cttacaaaaa ggagtatggt attatgaata
    14941 cagaagttaa tgttaaagca actactatta gatatgaaaa gatttcattt tttgaaagaa
    15001 tttcttatgg ttgtggggat ttggggtgca atatcatata ctcagctatg tcagcatttt
    15061 tgctgtttta ttatactaat tatgcagatg ttagtgcagc agctgtaggt agtattatgc
    15121 ttgtatctag gatactggat gggtttagtg acttaacaat gggaattata gtggatagaa
    15181 ctaaatccaa atacggtaag gctaggcctt ggattttgag aatggcgatt ccttttgcta
    15241 tagcagcagt attattattt tctgttccat caaatcttgg tatcacttct aaattaattt
    15301 atatttttat tacatataac ttagtttcta ctgttattta tacagcaatc aatgtaccct
    15361 atgcaactct aaattcactt ataactcaaa accagtatga aagaggagta ttaagtatat
    15421 ttagaatgat attagccact tgtggtacct tactaataaa tggacttaca ctacctctag
    15481 ttgaatactt tggaaataat ttatcagcat gggctaaaac attctttgta tttggaatag
    15541 catctataat ggtatttttt ataacattta ctggaaccaa ggaaagagta aaagcagtta
    15601 agcaagatag gaatgaagta ataccattta aaataggtat taaatcttta tttagaaata
    15661 agtactggat acaaataaca ttatgtttag tttgcatatt tattgtattt gcgataaatg
    15721 gtggttcatc cgtatattat gcaaagttta ttttgggaga tgaaaaatta tttggtccta
    15781 taaatatggt ttcaaatata tcccaaatta tagctatgtt tatggttgct ccttttataa
    15841 aaaaatttgg aaaaagaaat gtattaatag taggttcaat aatattaata tcgtcaaatg
    15901 ttatgtttat aatagttgga caagactata taggagtaat attttcaagt gctataaagg
    15961 gaataggaag tgctggaata gcatcaacaa tgtttgctat tgtttcagat actattgaat
    16021 atggagaatg gaagactgga tatagaactg aggggcttat aaatagtgca tctagttttg
    16081 ggtttaaagt gggaaatgga ttaggttcag caatattagg tgcagtatta tcaattggtg
    16141 gttatgtagg aacatcagct actcaaagtg atttagctat agtatcaata aaagtatgtt
    16201 ttatatacct acctatattt ataacaatat tacaaattat cataatgtct ttttataagc
    16261 ttgataaaga atatagtact atacttaatg aattaaatac taaataatga aaaagtttct
    16321 tataaagtct taagtttaat taaggtctat tgtaaatgtt ccctataaaa actattatat
    16381 atttatgatt attgaagaat ttttagagaa gcgaataata aaaatataag aagactttaa
    16441 atgatgaaat ggttggtttt ttagaaatga tttaataagt tattgttatt taaagcatta
    16501 taagtggaca attaattttt aatttatgga gatgatattt aacaattaga agtaatatat
    16561 aatatgcact aagaagcaat ttttcaacta tttatgaagg tggtggtaca agaaaagtat
    16621 cacctttttt ttgttataca aaaaaacagt tgacttttta tatgaacccc ttctataatg
    16681 ttactgaaat taagtaaaat gattgaacaa atgtaaagat aattgaaaag cttgattttg
    16741 gaggaaagca aatgaaaaaa attggagact taagattaat gatgaagtgt tgttcacttt
    16801 attacgaaga taatttgaat caacaggaaa tagcaaatca attgggaata tcaagaccaa
    16861 caatatcaag aatattaaaa gaagcttttg aacaaggaat agtaaaaata cagatagttg
    16921 atgttttgaa aaatgattac caaaaaatag agagaagttt ggagagaaaa tataaattaa
    16981 aagaagtaat agtagtagat gataaacagg atgcactaac acaaaaacaa gaactagcaa
    17041 gagctgtatc tgaatattta actagagtag taaaggaaaa tgatataata ggtgtttcaa
    17101 taggaacaac actaaaagaa ataccaagat atgtggaaaa aagtaactgt aaaaatgtta
    17161 catttatacc tctattagga gggattggag acaatgaaat agatattcat gcaaatcaaa
    17221 tagccgtaag tttagcgagg gcttttggag gtgattttaa attattacat gctccagctg
    17281 ttatgtctga cttgtctaca aaagagaaac tttgcaaaga cgaaaaaatt aaagaagtgc
    17341 tagatttaat agataaaaca actatagcaa ttgtgggtat aggaaatcct atgagtttaa
    17401 actcaacaat aatggcttct ggatacatga atgaagcaga tatagaagat ttaaaaaaat
    17461 acaactcaat aggtgcaatt tgtttacaag cctttgataa agaaggaaaa acttctatac
    17521 ttgaatttaa tcaaagagta ttaggagtga aactggaaga tttaaaaaag ataaaaagaa
    17581 caataggtgt cgcttcagga gatgaaaaaa tagaggcaat aaaggcatca ttaaaagcga
    17641 aatttataaa tagtttagca attaatcaca gtttagcact taaattatta gaagattgtg
    17701 actaatagaa agatagataa aagttaacag gagggaatgt atatgtcaat atcaaaagaa
    17761 acattgcttg aaatgtataa gagaatgaat caggctagaa aatttgaaga gaaagttagt
    17821 tggttttttg caagaggtat ggttcatgga acaactcact tatctgtagg tcaagaagcg
    17881 tcaagtgtgg cggctgtaat ggctttagaa aaaggagatt tagtatcatt aactcataga
    17941 ggacatagtc aatttatagg aatgggaata gacctaaata aaatgatggc tgaacttatg
    18001 ggtaaagaaa ctggattttg taagggaaaa ggaggctcaa tgcatatagc agatattgag
    18061 tcaggtaact taggagcaaa tggagttgta ggtggaggtc ttacaatagc tccaggagct
    18121 gcacttacac aacagtataa aaaaacaggc aaaatagttt tatgtagttt tggtgatgga
    18181 gcatcaaatg aaggtacttt tcatgaagga ataaatttat cttcaatatg gaaattgcca
    18241 ataatatttt actgtgagaa taatttatac ggtatgtcaa caagcataaa acgtcatatg
    18301 aatatagaaa gtattgccac tagagcagct tcttatggaa tagaaggaat atcaatagat
    18361 ggatataacc caatagaagt atacgaaaca gtacaaaaag ctgctgagaa gtgtagaaga
    18421 ggagaaggac ctgtattaat tgaaagtaga acttatagat ggttaggtca ttcaaaatca
    18481 gatgcaaatg tatatagaac aaaagaagaa atagaatcat ggaaagcaaa agaccctatt
    18541 gaatttttaa aaaattatct tatagaaaat aatctatcta atgaagatga attagataag
    18601 attcaagaat ttgcaaagca atcaatagaa gatgcagtag agtttgctca aaatagccca
    18661 aatccaaaaa tagagtcttt attagaggat gtatatgcag actaagaatg gtaaaggagg
    18721 aaaataaaaa tgagtacaag ggaattaaca tatgcacaag caataaagga agcaatgtca
    18781 gaagaaatga gaagagatga aaatgtaata tttatgggag aagatatagg aatatatgga
    18841 ggagcatttg gtgtatcagt tggtatgata gatgaatttg gaccagaaag agtaagggat
    18901 actcctatat cagaagcagc tatagcagga gcggcagcag gtgcagcagc aactggctta
    18961 agacctatta tggaagtaat gtttatggac tttgtaacaa tttctatgga tgcaattgta
    19021 aatcaagcgg caaaaatgag atatatgttt ggaggaaaag cacaagttcc aatggttgtt
    19081 agatgcccag gaggttctgg aactggttca gcagagcaac attcacaaag tttagaagct
    19141 tggttttgtc atgtaccagg agtaaaagta gttgcacctt ctacaccagc agatgctaag
    19201 ggattgttga aagcagctat aagagataat aatccagtca tatttgttga gaataaattg
    19261 ttatacagaa aaaaaggttt tgtgccagaa gatgattatg taattgaaat tggaaaggca
    19321 gatattaaaa gagagggtac tgatgttaca gtaattactt atggaagaat gttacaaagt
    19381 gttgaagaag cagcagagac tctatcaaaa gaaaatataa atgttgaaat tatagattta
    19441 agaactttat atccattaga taaagagact atagtaaaga gtgtatgtaa aactgggaga
    19501 gttttaattt gccatgaagc agctaagact ggtgggctag gtggagaaat atcagccctg
    19561 ataacagaaa gtgaatcatt tgattattta gatgctccag taaaaagaat ttgtggaaaa
    19621 gatgtgccaa taccatataa tccagagtta gaaaaagcag ttgttccaag agttgatgaa
    19681 atagaagaag caataaaatc tttaattgtt agataaggag ggatagatat ggtggcaaat
    19741 aaaataaaag ccacaccagc agctagaagt caggcaagaa aagacaatat aaaattagat
    19801 aggctgattg gaagtggaga aaatggaagg attcatttag tagatgtgtt aaattattta
    19861 aaagataata aagcaaatac tactccttta gctagaagaa tagcagaaga tttaaatata
    19921 gatttagaaa ctattgtagg tactggatat aatggaaaga taagaaagtg tgatgtggaa
    19981 aaattaactg gaaaagaaac tattgtatca actaatacat ctaaatctag tgagaaaaaa
    20041 gagctgaaaa ttgaaaatga aaattcaaga atgtttaaca cagtagaagg aatatttgaa
    20101 aagccaaatc caatgagagc tactgtagca aaacgaatgt cagaaagtta ttttagtgct
    20161 cctgtattta cattcaatat agaagtagat gctacagaat tgaaggtact tagagcaaag
    20221 ttaatcgaca cagtaaaaga atcaactgga gtaaaattaa ctatgacaga tttaatagta
    20281 atggcagtat ctaaaatact tccaaatcat caagccttaa attcagcatg gacagatgag
    20341 ggtatattta gatataaaga tgtaaatata gctattgcag ttggtttaga tgaaggctta
    20401 tatgttccgg tagtaaaaaa tgcaaacaaa aagtctttaa aagaaatagc aaaagaaagc
    20461 aaagaacttg cggaaaaagt aaagacaggg aaacttatgc cagcagacca agaaggtaat
    20521 acatttacaa taagtaatgt tggaatgtat ggtataacta catttacacc tataataaat
    20581 atgccttcaa gtgcaatctt aggggtaggt gctacacaag ataagtttgt tccagtaaat
    20641 ggagaggcta aaataaaacc tataatgaac ttatcattaa catcagacca tagagtaata
    20701 gatggaacag tagctgcaaa attcttaaaa gatttaaaag aattgttgga aaatccttta
    20761 tcaatgcttg tataggtttt gatattttgt tactgatata aatagtttga atagattata
    20821 ttattataat tgtttcagta aatttaaagc ttttatatat taagaattat ttttaacagt
    20881 aactatatgt gaatatgatt tgcttaatta aaatataaga aaataaatag gaggagagcc
    20941 tatgtcagta gaagtaataa tgccaaaagc aggagtagcc atggaagaag gtactatagt
    21001 atcttggcta aagcaagaag gcgaagaagt aaagattgga gaacctatac tagaaataac
    21061 aacagataaa gttaatatgg agatagagtc agaaggagaa ggtacacttg ctgtaatcat
    21121 acataaagag gaaggtgaag tattaccagt atttacagta ataggtgtaa tagcagaaaa
    21181 aggggaaaat caagaggaag taaaagctaa atatttatct ggaaatgttt caaaagaaga
    21241 tatagttgaa gagaatcaaa atatagaatt taaagaagaa aaaataaaca aaaaagagtg
    21301 taatcatgat tatgatgtag tggtaatagg aggaggacct gggggatatt tatcagcatt
    21361 aaaagcagct cttttaggtg gaagagttgc tttagttgaa gaaaatatac taggaggtac
    21421 ttgcttaaat agaggttgta tccctacaaa gacttatata aaaacagcag aaatattaga
    21481 agaaattgac caattatcaa agagaggtgt aaaagtaact gtagataaag aacaggacat
    21541 taagaaggcc ataaaatata aaaatagagt agttaaaaaa cttacagctg gtgttggcgg
    21601 attattaaaa agtagagatg tagatgtgtt taatcttaaa gctagtgtta aagaagagca
    21661 taaagtaatt ttatcagatg gaaaagtttt agatacagaa aatataataa ttgctacagg
    21721 ttccaaagta aggatattac ctataaaagg aatagaatca aatttaataa taactagtac
    21781 agaagcatta gatttggaaa cagtgccaga agaattagta ataattggtg gtggagtgat
    21841 tggttgtgaa tttgcagaga tattcaattc aagaggttct aaagtaacta tagtagaaat
    21901 ggaagataga gtaatcccaa gaatggataa agagttaagc gaatctttaa aatattctct
    21961 tagtaaaaaa ggtataaatg tattaactaa aaagaaagtt tctgaattta aagaagaagg
    22021 aaataacata ttagtgtgca ttgaaggtga agagccaata aaagcagatt tatgtctata
    22081 tgctatagga agagaagcta atctttctgg catagaggac ttagatatta aaatagataa
    22141 aggttcaata gttgtaaata gtaaaatgga aacaagtatg ccaagcatat atgcagtagg
    22201 tgatgttact ggaggtgtaa tgttagctca tgctgctttt aaaatgggag aggtagcagc
    22261 ttcaaatgct ctaggtgtga ataaagaagt tgacttaggt gcattaccta gttgtgttta
    22321 tacaattcca gaagtagcat cagttggtat tacagaagag gatgctagaa aaaaatataa
    22381 tgtaaaagtt ggtaagttta attttgctgg aaatggaaga gctttagctt ctggtcaaga
    22441 acaaggatat gtgaaggtag tagcagatgc taaatatgga gaaattttag gtatacatat
    22501 gtttggatgt ggtgtagcag aacttataaa tcatgcagca tcatttaaag cgttagagat
    22561 acctactgat gaagcaagtg agttgatatt tggtcatcca tgtacttcag aagctttaat
    22621 ggaagctttg gcagatgtaa atggagaatg tttacactta cctaaaaaat aaatctaaac
    22681 ataaattatt aatttaagtc tattattaat attaatttat atcataaaat tttaaaacac
    22741 ttacttagcc atacccctta ttatgcaaat aatttatttg cataat
//
LOCUS       DACXMZ010000042        22725 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.042, whole genome shotgun sequence.
ACCESSION   DACXMZ010000042 DACXMZ010000000
VERSION     DACXMZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22725
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.042"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(61..501)
                     /locus_tag="JD016_06375"
     CDS             complement(61..501)
                     /locus_tag="JD016_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HAU4999260.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
     gene            complement(707..1084)
                     /locus_tag="JD016_06380"
     CDS             complement(707..1084)
                     /locus_tag="JD016_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAU4999261.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            complement(1200..1694)
                     /locus_tag="JD016_06385"
     CDS             complement(1200..1694)
                     /locus_tag="JD016_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAU4999262.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            complement(1843..6096)
                     /locus_tag="JD016_06390"
     CDS             complement(1843..6096)
                     /locus_tag="JD016_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HAU4999263.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            complement(6476..7603)
                     /locus_tag="JD016_06395"
     CDS             complement(6476..7603)
                     /locus_tag="JD016_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999264.1"
                     /translation="METFIFLILLVVGFGYVGSIMGAGMMFKVIMSTAHALLLETVFL
                     IMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIAGAITTYLSDNPAII
                     PFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEYVLPALIGNLGAIIG
                     SVISVRIMLTFTKKFYKYSPEEDKATGTLEKKDEFREIREGNVFQRALDAILEGGKMG
                     VDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGIKLLPVIGDKLGFIL
                     EPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIAVFTAMGMCWSGYLS
                     THIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            complement(7614..7685)
                     /locus_tag="JD016_06400"
     CDS             complement(7614..7685)
                     /locus_tag="JD016_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999265.1"
                     /translation="MLNFITKKEGMIQNGEVNRQETG"
     gene            complement(8040..9056)
                     /gene="gap"
                     /locus_tag="JD016_06405"
     CDS             complement(8040..9056)
                     /gene="gap"
                     /locus_tag="JD016_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAU4999266.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            complement(9693..10157)
                     /locus_tag="JD016_06410"
     CDS             complement(9693..10157)
                     /locus_tag="JD016_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HAU4999267.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            complement(10481..11089)
                     /locus_tag="JD016_06415"
     CDS             complement(10481..11089)
                     /locus_tag="JD016_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAU4999268.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGFHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            complement(11171..11872)
                     /locus_tag="JD016_06420"
     CDS             complement(11171..11872)
                     /locus_tag="JD016_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999269.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            complement(11890..12003)
                     /locus_tag="JD016_06425"
     CDS             complement(11890..12003)
                     /locus_tag="JD016_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAU4999270.1"
                     /translation="MKYLILIGKFKKIKQLANRGYIYKTQSDKIYLENNVK"
     gene            12084..12350
                     /locus_tag="JD016_06430"
     CDS             12084..12350
                     /locus_tag="JD016_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAU4999271.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            complement(12453..12872)
                     /locus_tag="JD016_06435"
     CDS             complement(12453..12872)
                     /locus_tag="JD016_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2992 family protein"
                     /protein_id="HAU4999272.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(13139..13921)
                     /locus_tag="JD016_06440"
     CDS             complement(13139..13921)
                     /locus_tag="JD016_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAU4999273.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKKIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            complement(13986..14747)
                     /locus_tag="JD016_06445"
     CDS             complement(13986..14747)
                     /locus_tag="JD016_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HAU4999274.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNKKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            complement(15252..15386)
                     /locus_tag="JD016_06450"
     CDS             complement(15252..15386)
                     /locus_tag="JD016_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAU4999275.1"
                     /translation="MDLINTIDKVQQAHKPIDEIIKTIRDIVDIIKILEPIYNFKSNQ
                     "
     gene            complement(15796..16626)
                     /locus_tag="JD016_06455"
     CDS             complement(15796..16626)
                     /locus_tag="JD016_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAU4999276.1"
                     /translation="MLNKLCMYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGA
                     SRNFNFIEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSIN
                     YAQNRNKETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSL
                     KPGGKLILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTL
                     VITEKDENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTIGLVLQK
                     "
     gene            complement(16978..17880)
                     /locus_tag="JD016_06460"
     CDS             complement(16978..17880)
                     /locus_tag="JD016_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAU4999277.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSEGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            complement(17930..18391)
                     /locus_tag="JD016_06465"
     CDS             complement(17930..18391)
                     /locus_tag="JD016_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999278.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(18443..18898)
                     /gene="def"
                     /locus_tag="JD016_06470"
     CDS             complement(18443..18898)
                     /gene="def"
                     /locus_tag="JD016_06470"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAU4999279.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVGGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(18870..19133)
                     /locus_tag="JD016_06475"
     CDS             complement(18870..19133)
                     /locus_tag="JD016_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999280.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(19230..19946)
                     /locus_tag="JD016_06480"
     CDS             complement(19230..19946)
                     /locus_tag="JD016_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4999281.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(19943..20626)
                     /locus_tag="JD016_06485"
     CDS             complement(19943..20626)
                     /locus_tag="JD016_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4999282.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            complement(20623..21468)
                     /locus_tag="JD016_06490"
     CDS             complement(20623..21468)
                     /locus_tag="JD016_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4999283.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(21582..22172)
                     /locus_tag="JD016_06495"
     CDS             complement(21582..22172)
                     /locus_tag="JD016_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAU4999284.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            22461..22694
                     /locus_tag="JD016_06500"
     CDS             22461..22694
                     /locus_tag="JD016_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAU4999285.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT         7508 a         3838 c         2202 g         9177 t
ORIGIN      
        1 ttttattatc ttagttataa ataattgtaa ctagataata ttcaatttct atattaatat
       61 ttaactaata ttagataatg tatcgtttat atccttttct actaatggtt tcatattatt
      121 attataaata cttatatctg cattacttaa ttccattttt atgcgattaa taagattttc
      181 tttatacttt gtaatattag gtatggattt aataaacatt cttgatacag aaggtttttt
      241 atctacgata tgacttagta tactatctat atttatttca attttattat caatatccca
      301 ctgagcattt gcagagatta ataacaatcc ccttgttcga atatatgagt tttcgttatc
      361 aatcatttct aggaatgagt caaaaaaaca atatgtaata tttgatttta aactttcatt
      421 ttctaagaat aaaaaagttt tatatgcttc attatgattt tttgaatata atttattaat
      481 aagtaaattg agattcttca atcttctcat ctccctcatt acaaatattt atttaaacaa
      541 agtataacat attcaaaaat aaatataaat aaaaactata ttaaataaaa agcctaaaat
      601 tatttgctgt ttatttacaa ataataattc taaaatatat ttgatttatt tagtatcggc
      661 tataaaaata agctatcaaa taatttatat ttgatagctt attttcttat aattcttttt
      721 ttaaagtttc ctttagacta tcatatgcga aatccattat ttctctacaa gcaactaaat
      781 ctttttttag gtctatacat aactctgaac catactcttt tcttactgtt gtagttaaat
      841 ctttagcata tactttagct ttagtacttt catcttcgtg gtttctgcct tttacatatc
      901 caattattat atttgatgca tttactgcac cacataaact agcaacccca caccctgaac
      961 ccagtccact tcctaaacaa actggtatat ctgtattaaa ttcctcatta tatgctttta
     1021 taactgattc tgcacaatta aatcccttct catgatattt tgatgctata actttatcac
     1081 acattaaaat tccccctcat ttttgtaaat taaaataaat tcttcattgt ttttgtaaaa
     1141 aatacttctt tttactatta ttatatcaaa tattaacaat aaaaacattt ctttctaagt
     1201 taactctgtc tttctaaatt taatagtatc tctttcaaat cagaatgatt ccccatatat
     1261 ccaccaatat cttttttgcc aactactcta tgacaaggta ttattattgg aattttatta
     1321 cttttacttg cttgtcctat tgctcttact gattttggat tgtttatatt tcttgctata
     1381 tctgaataac acaatgtttc tccataaggt atttttaaca tttccctata aactgatttt
     1441 tgaaattcag tcccttcaat acgaatattt aaattaaaga cttttctttt tccattaaag
     1501 taatcatcta tctggcttat tgcttccttt aaatcttcct tacttctctt tatatcaatt
     1561 ccttttttaa tacattcatt ttttaaatta ttaaattttt catctcctat gtatatacta
     1621 actataaaac catcttcact taatatgtac aaatttccta attttgattt ataaacatca
     1681 tatactaaac tcatattact tccccccttt aatatcagta tacatataat tttttaacta
     1741 ataaagagtt accctaaaaa ataggataac tcttttttat agatacttaa tatagagtat
     1801 atttttaaat tcattaactt ttaacatatc aaaatataaa tcttatatat ttttaaaagc
     1861 cttagaaagc ataagtttta tcctgttaag ttgatttacc tcacttgcag aagggtcata
     1921 atcaataggt attatatttg catttttata caacctcttt aattctttta tagaaccctt
     1981 accaattatg tgattaggta aacatccaaa aggttgcata caaataatgt tttctacacc
     2041 ttcatttaat aattcaacca tttcacctgt taaaagccaa ccttctccag tttgattacc
     2101 taatgacact actggttctg tacttttagc aacatttttt atattggctg gagcataaaa
     2161 tctctcactc ttatcaagtg cttttttata tactctttga tatattccag ttatgtatat
     2221 aaatgcctct cctatcattc tactctttct acttccttct aaatgagtgt gtttgtatat
     2281 agtattaaag gcacaactta agaagaaatt tgtaaggtct ggcacgactg cttcagcacc
     2341 ttctcgctct aatatatcta ctaaattatt atttgcaatt ggatggaatt tcaccaatat
     2401 ttctccaacc aaacctactt ttggtttttt gatatctagt atctctaaat tatcaaattc
     2461 ttttactata tcttttatat ttctagtaaa tacagaaatt tttgctttaa caagagaaga
     2521 tttgcatata tcaacccatt tttcatatag agcatttgca cttccagtaa ctttttcata
     2581 tggtctaact ctatatagta ctttcatcag caaatcacca taaacaacag acataaagag
     2641 tctattgatt aattttagtg atatattatc cattatacca ctatcttcta ttccatttac
     2701 actaagtgca atgacaggaa catctttaaa tccagcatcg tatatagcct ttcttaagaa
     2761 tccaatataa tttgtagctc tacaacctcc accagtctga gttatagtaa cagaagtgtt
     2821 atttaaatca tatttgccag attttaatgc tgttattagc tgacctacaa ctattatagc
     2881 tggataacat gcatcattat ttacatatct taacccttct tcaattacac tactatcagt
     2941 atcttttaga atttctatat tataaccact taagttaaca gctttttcaa ggaattggaa
     3001 atgtattgga gacatttgtg gtgctaatat agtatgtttg ttcgttatct gattattttt
     3061 agcatactgt atcctgttta cctttatatt ttttacatct atatttttgc tttctctctc
     3121 aaacatagct gcttttaagg acctcatcct tatcttagct gctcctaaat tgttgccttc
     3181 gtcaatcttt ataatagtat atatcttcga tttttcatta agtatttctt gaacctggtc
     3241 tgtagttact gcatctaatc cacagccaaa tgaattaagt tgaacaagtt ccaaatagtt
     3301 tttatctctt acaaaactag ctgctttata caatcttgaa tgatataccc attggtctac
     3361 aactctaagt ggtctttgta cattggctaa atgacatata gagtcttcag taagcactgc
     3421 catatctaat gaatttataa gctctggcat tccatgattt atttctgggt ctatatgata
     3481 tggtcttcca cataacacaa tccccttcat atttctttct tttatttctt taagtgctct
     3541 ttcacctgct gcttgtatct ccattttaaa taaattctgt tcatcagtag ctttatcaac
     3601 tgcatgatta acttcttctt tagttatttt tatatcagaa aaatgtcttg ataattcatc
     3661 atagagtctt ttcttcaatt tttccttatc atttagactt ataaatggat ttatataatt
     3721 tatgttctta tttcttatat tctctacatt gttttttatt acttcagaat atgatattac
     3781 aacaggacaa ttataataat tatctgcatt tgaatcctct ttattttcat ttgttacact
     3841 tggataaaat atatttttta taccttttgc aattaaattt tctatatgtc catgaactaa
     3901 ttttcctgga taacaaactg tttcagatgc aatactagtt ataccagttt catataactg
     3961 ttttgatgac ctatctgaaa gtacaactct aaagcccata gcattgaaaa aagtaaacca
     4021 aaatggataa tcttcataca tatttaatac tcttggtatc cctatttccc cattttttgc
     4081 ttcttctttt tccaatggtt tgtatctaaa aattctattg tatttgtact taaataaatt
     4141 tattggtttt ttcttttcta atcctatttt tttatcacca taaatagctt cacccttctc
     4201 acatctatta cctgaaataa aaatttcatt atcagaaaat ttatttattg taagaagaca
     4261 atgatttgaa caacctttac atctagttac acttgcttct agcttgatat tattcaactc
     4321 ttccttagat aacaaggatg ttttgtatcc actttcatat ttttctttag ctataagtgc
     4381 tgaaccaaaa gctcccataa ttcctgaaat attaggtcta attacatttt ttccaattag
     4441 tttttcaaag cttctaagca ccaaatcatt ataaaaggtt ccaccttgta caacaatatt
     4501 attaccaatc tcatttaaat ctctaatctt tattacttta aataaagcat tttttattac
     4561 tgaataagat agccctgctg atatatctcc aacacttgca ccttcttttt gagcttgttt
     4621 tactctagaa ttcataaata cagtacatct tgaacctaaa tctacaggat tttgagaata
     4681 aataccttca tgcgcaaatt cttctattgt catagataaa gaacttgcaa atgtctctaa
     4741 aaatgagcca caacctgatg aacaagcctc atttaatatt atattatcaa ttactccatc
     4801 ttttattttt aggcacttca tatcttgacc accaatatca agtataaaat ctacatcttt
     4861 gttgaaaaat ttagcagctt tgtaatgtgc tatagtctct atctctccat tatcgatttt
     4921 aagagctttt ttaataagac cttctccata tccagttaca gttgaactca atattttaat
     4981 attttttggt aatatatcat atacttcatt tataacttta atggttgttt ttaatggatt
     5041 tccttcattg ctattataat aagaatatac aagtcttcca tcctcatcaa taagtgctgc
     5101 tttagttgta gttgagcctg catcaattcc taaataacaa ttgcttttta ttgaatttat
     5161 gtccacataa tttatttttt ctttttcatg tcttcttgaa aattcttcat actcattttt
     5221 atctttaaat aaaggttcta ataaatttac ttctccatct tcaatattct ttatactatc
     5281 caatttttct attagagatt ttagttgtat attatcttca tctactgata ataatcctgc
     5341 tccaatagca atataaagtt gtgcattttc tgggaagatt atgttttcat cttttaaatc
     5401 taatactctt ttaaatgctt ctctaagttc agataaaaag tacaatggac cacccaaaaa
     5461 cgcaacattt ccttctattt ttcttccaca agaaaggaca ctcacagttt gtacgacaac
     5521 tgcattgaaa atactcattg ctatatcagt ctgattggct ccatcattta taagaggttg
     5581 aatatctgtt ttagcaaaaa caccacatct tgaagctatg ggatatataa cattataccc
     5641 tttagcaagt tcatttaatc cactggcatc tgtctttaag agtgatgcca tctggtctat
     5701 gaatgctcca gtgccacctg cacatattcc attcattctt tggtctattc ctccacttag
     5761 atatgttatc tttgcatctt cacccccaag ttctatgaca acatcagtct caggatgaaa
     5821 atactctatt gattttgtac tagatataac ttcctgaaca aattttacac ctaatttttt
     5881 tgatattcca attccacctg aaccagttat tataatggta gtatttatat ctcccaattt
     5941 ttcatatata ccatttagta cttctttaac tgattttttt acatcagaat aatgtcttct
     6001 atattcttta tgaactatat tattactatt attaagtacc acaaccttca ccgttgtaga
     6061 acctacatca attcctatat gaaataattc atccattgta cccctccata tttatatgct
     6121 tttttaattt ttgtaagttc tatggcttta ttttaagtaa gttatttata atctttatga
     6181 ctaatatttt tattttatga ttcttttatt aatcaaagct tactctacta ggtaattcct
     6241 aattctaacc ttaaatttta aataagaaca tatagttagt gtactaaatt ttgtcataaa
     6301 tttctacaga tttttgttac tttttacata aatataactt aactctaata tccacttaat
     6361 gtttatgtat tataaattat ctttagtcag aaaaaaatgg ttgttgatat gtaaattctt
     6421 attacatatc aacaaccatt ttttgtatat tattttttat cttagctgat tacatttatc
     6481 ctacgagtgt aaatataaag tgagcagctg ctcctgcaca taaaccacct attgtatgag
     6541 aaagaatagc tttacctgcc aactgtctag catttaatgc atccatcata ccaatatgtg
     6601 tacttaaata tccactccaa cacataccca tagctgtaaa tactgctata tcattaggag
     6661 ttatagctcc tgactttata aactctggaa ctagagacat tgctgcgcca acagctccca
     6721 atgaagttat agggaatgct attgcctctg gtgatgtaaa tccaaataat ggttctaata
     6781 taaatccaag tttatctcca ataactggaa gtaattttat tccttcatat gcagctcctg
     6841 tataaacttc ttgacctgta acagggtctg tagatggtcc aaaagttaat aacatcacca
     6901 atgtacatac tactaggact ccaggaatta tagccattcc catatctacc cccattttac
     6961 caccttcaag aattgcatct aaagctcttt gaaatacatt tccttctctg atttctctaa
     7021 attcatcctt tttctctaat gttcctgttg ctttatcttc ttccggactg tatttataaa
     7081 acttcttagt aaatgtcaac attattctaa cacttatgac actacctata attgcaccta
     7141 aattaccaat taatgctgga agtacatatt ctgtaccttg agaaatcata aatgttgtta
     7201 agataagtcc cataccaaag gctgttccta agttacataa tgctggaact tggtattgtt
     7261 taaaatactg tgtgaatgtt ttatctttag caaaaggtat aatagctgga ttatctgata
     7321 aataagttgt tatagctcct gctatactag caccaggtag tccatataaa ggcttcataa
     7381 atacagcgaa aattttatta attaacgcta ttactccaaa ctctgaaagt agtgcgctta
     7441 atgcgcctgc aagaactgcc atagccataa ttaagaaaac tgtctccaat agtaaagcat
     7501 gtgctgtact cattattact ttgaacatca ttcctgctcc cataatacta ccaacatacc
     7561 caaaaccaac tactagtaat attaggaaaa tgaaggtttc catgcttact gccttaacct
     7621 gtctcctgtc tgttaacttc tccattttga atcatccctt ctttttttgt tataaagttt
     7681 aacaaaacct tttctctttt ttaattatac ttataagtat tactatttgc aactttattt
     7741 gtcatttttt tatcattttt aaaatggaaa tcagtatttt aataataaaa atttttcaga
     7801 tttatgcaag ttttcgacat cttatctttc attttataaa aagtatgatt ccttactaaa
     7861 tatatatata cttctctaac gacctaatat gcaaatataa gttgtaactt gtatcttttt
     7921 gcatgaaaga aaaaagatta tgaaaaaatt tttccttcaa tatttttcat aatctttttt
     7981 tttagtttta tataattttt ttaaaaaatt tttttaaaat ttgctttaag aaatatatat
     8041 tattttaatt tttcagctac atattttact aagtcaacag ttcttgaaga atatccccat
     8101 tcattatcat accaagaaac taccttaacc atattgtctt ctataaccat tgtggataga
     8161 gcgtctataa tagatgatct tgaatctcct ttataatcta tagaaacaag tggcttgtca
     8221 caatatccta aaacaccttt taattctcct tcagcagcat cttttaacac agagttaact
     8281 tgctcagatg taacgttttc tttaagttcg cacactaaat caacaagtga aactgttggt
     8341 gttggtactc taagtgaaaa accatttaat tttccttcta attgtggtag tactcttgac
     8401 acagcttttg ctgctcctgt agtggtaggt attatagatt cagccgctgc tcttgctctt
     8461 ctcaaatctt tatggctttt atctaatatt ctttgatcgt tagtatatga atgtactgta
     8521 gtcattaacc cttttactat accaaatttt tcgtccaata ctttagcaaa aggagctaga
     8581 caatttgtag tacaagatgc atttgaaatt atattgtgct tttcattatc atattctttc
     8641 tcattaactc cgataactat agttatatct tcattttttc ctggagctgt tataattact
     8701 ttttttgctc ctgctttaat atgtttttct gccttttctc tttgtgtaaa taatcctgtt
     8761 gattctatta ctatatcaac ccccaattct ttccaaggta attcttctgg gtcattatat
     8821 cttaatattt ttatctcttt agagtttact actagagtgt cttcatcctt tgcctctact
     8881 tcacctctaa aagttccata gcatgaatca tatgtaaata aatgtgccaa agattctgtt
     8941 gttgctcttg catttatggc tacaatttct atattatctc ctaattcttc ttgagctatt
     9001 cttaatacag ctcttcctat tcttccaaat ccgttcattc ctaccttaat tttcattatg
     9061 aaatgtctcc ctccattact atgtttttac atgattattg tacactaaat aaatcatttg
     9121 ttcaatactt tttcttgatt aaataatcaa attccatgtt tttttattat caaaagacaa
     9181 ttagtatatc atttttcttt attttacccc tcatttagta tgtacttttt agtgtaaata
     9241 agctgaaatc atttatttta ctcaatgtcc cttgggacat attttgccat cttttttttg
     9301 aatttttaaa ctatacttat ttttaaaaat aatatatctt caagaatctc ctctaattta
     9361 aaataataaa aataaatttt tatttagttc aaaaaatata tgatttttta aaatttgcta
     9421 caattattat ttatatatca aaatataaat aataattagc ttattgttaa ctaaattatg
     9481 gaaatatact taattgaaaa cttaaattta tttccttgta ttatcaagat ttcatgctta
     9541 ccatttccaa tatcatgctc accaactttt gatgtgcata aactatttca agtaaaaatg
     9601 cacaacctat cctttttgat aagaaaaagc agtcaatcat aaatatcaac tgcttttatg
     9661 tactgaatat caagttatag aaaagctgga atttattttg agggtttttc tttctcctta
     9721 actaaattgc tttcaaaagt aacctttaca actgcatgat ttattttaat tccagtaaag
     9781 catatagcat actctttctc tttttgtata ttagaaagag aaattgtgaa tgtatcatta
     9841 tcaacgcttc catgccatgt attactccat aaagtttcat ctgttttatt gtcctttatt
     9901 tccacaatac cactatctcc atccatttta aaagaaatct ctgtgtctaa aacatctata
     9961 tcattagaca cacaaaacaa acgtgcatta acaaatgggt ctgaagtatc ataattttta
    10021 tctaagtcca tttcaatggt tgtttgtccg gattctttgt tgtttaaatt attatattta
    10081 ctttcattgc tttttttaca agctgtcaag ctaaccatac taatgatact aattaaaata
    10141 ataactaatt ttttcataga atttatctcc cttacaattt ctaattttta accctacaaa
    10201 ctatagtatt aaactaagct actattttac atcaaatttt tcaatcattc agcctttacc
    10261 ttttctcatt ttaattgcaa atatgttaaa taattttgga attttaatta ttgtagtatc
    10321 ttaaattggt ttataacata ttatgaaaat ttaagagtca agtcacttat ttaaaaatac
    10381 acaaaattat actttattgt ataaaaaaag agattataat ttatacaagt aaaataaact
    10441 ttattatttt actcttctat ataacctctt ttctatatat ctattcaaat aatttagctc
    10501 ctaaactcac aaacatttgt aaaatcaaaa atgcttttgt gtctatctta tgattacttt
    10561 gtaaaatctc ttttgcatct tcttgcgaaa ttaaaagcgc ttcaatctct tcatctggtt
    10621 ctaaaaattc atctgttata tcaccatcac atatacaata aacaaaggca acagattcat
    10681 cactcattcc aggagataaa taaactttat caatactttt atttttttta atttctaaaa
    10741 gagtaagtcc tgtttcttct ctaagttctc tttcaacgga aatatctata gactctcctt
    10801 catcaacaag acctgctggt aactcgtata tatatccatt aataggtact ctaaattgtc
    10861 ttattaaaat taatttttta ctacttttat gaaacccaac tattacaact gcatctacac
    10921 tatcttcttc attcttcaaa taaatatttt ttaattgctc actacttttt cttgatgcta
    10981 ccatccaaac cttatcttca tctaatttgt ttttatactc tatttcaaaa agtcctataa
    11041 acttattttc aatcattgga tttacattct ttattatagt ttttttcaaa tttactacga
    11101 cctttctttt ttaatacctt tttaacatca ttaagtaaat ataatatctt aacaagtatt
    11161 caacaactta ttatatttcc atttcaggat tatctttatc tacaaaacct actttaattt
    11221 cacaattaaa tatttccttt aacaattttg ctaattcttc tcttgcactc attaccttat
    11281 ctaaattagc atcagtaaaa ccatctatag gaaaaaatat attttttgag ctattagtaa
    11341 tttttccttc ttctccttgt ctccatatcc atcttctagt ttttaccatg tctccaactg
    11401 aataaattaa ttctccttct tccattaatt caacatcagt ttctccaaat ggtacaaatt
    11461 tatccccttt tttagaaaat ctaacatata catcatcatt tctaaaatcc atatcatgag
    11521 cacccattgg aagtaaatat tttaaagaaa tagagtttcc taaatctaca ataggattta
    11581 tatgaggaag tttcttattc ttagcaaccc ttgttgtcat tgcttctatc gaactcataa
    11641 atttatttgg gttcatgcct agacttctaa atgcctctct atatggtata atttcttctg
    11701 attcctttac ttttttatct ttgaagtaat cttctactct atctatactt atatctaaaa
    11761 ggttgcttat tctttctatt tctttagtat tatctattcc ttttgcaact actacaccaa
    11821 agcatacatt ttctaattta tcaaaaactt ctttttctac tataaatttc attttgcttt
    11881 atcctcctgt tattttacat tattttctaa atagatttta tcactttgtg tcttataaat
    11941 atacccccta tttgctaatt gtttaatttt tttaaatttt ccaattaata tcaaatattt
    12001 tatatgtact aattacaaat ttaactttcc aaaacaaata tcttgtttta taatgtataa
    12061 tatattttta tggaggcaag actgtgaaaa attttattct aatatatttt atagtgatta
    12121 attctattgc tttcttttca atgtacatag ataagaaaag agcaattaga aacgaatgga
    12181 gaataaaaga agctactctt atgtctatag cagtaattgg aggtagtata ggttctataa
    12241 ttggaatgta ttcttttaga cataaaacta aacacattaa atttacattt ggtataccat
    12301 ttatactatt tttacaattt ttattagtat atttttatat tttaaagtaa ttgaaatata
    12361 aaaccttatt cttttcaata aattaaggtg tataaaatta aaaattttat acaccttttt
    12421 ctagcaatta ttaaaaagat atttaattaa aattaatgac ccttatgttt agcttttctc
    12481 ttttcttgtt tcaaattaaa tagtctttct ttttcttctt cttttctatt tttagctagg
    12541 ttttttctat cctgttttct ttcttcatat tgaagcttaa gagcattttg agccttagtc
    12601 cctatagttt ctttttcttg ttcctttttt acttttctct gcattctttt gtatccaatg
    12661 ttgtctttgg tcacattttt ttctaattta atgctaccaa aatctaatga gaaaaatctt
    12721 tcaagtataa attcatacac ttctacttct ttaggttctg ctccaaaaac taccctacat
    12781 gcttcatatt ttttaccatc ttgtctttca aatataccta cccaaaatgg ttcttcaaat
    12841 aagactgtta attttccact aattttgtcc attgctagtt cctccttaaa ttactttcta
    12901 aagaacggac aaccaaggag gcaggttact gacagccatt gctacgtctg gactaccaac
    12961 cagactgtgt ttttatcttt atagattaaa tattatcttt tttaatcatg cttgtcaatc
    13021 ttagtatcaa catctatcat attgatttaa gtgaaatcaa gttttttaac tctactttat
    13081 tattgttaac actaagaaaa ttaattattt ttcataataa attagttatt atacttattt
    13141 aaatatcaat ctttattgtt ccctgcataa acaagactgc ttcaccagaa attttcacag
    13201 tattatcctt caacacttca cactgaatta taccaccacg attagaaagt tgagcagcta
    13261 ccattttgtg ttttcccaac ttttcagacc agtatggaat aagattacaa tgtgatgaac
    13321 cagtaacagg gtcttctgaa tctaattctg gacaaaaata tcttgataca aaatctgtat
    13381 tacttccttg tgctgtgatt attataccta accagtctgt caatttacgc aattgtgcgt
    13441 aattaggttt ataatttata acttcttgtt cactgtttag caataagatt aaatctcttg
    13501 aagaataaac agcacttggt acacagccta tcaaattagc ttgttgcggt gataactcta
    13561 tttcaatagg cattatttct ggaaatatca tttcatatag atttccattt cgtgtaactt
    13621 caagctttcc actttgagta aagaaatcta tcttttttac attaacatca ataaaatttg
    13681 atattacata agctgctgct aaagttgcat gaccacataa atcaatttct gctttaggag
    13741 taaaccatcg taattcataa tttcctttac ctttaactac aaatgctgtt tctggtaaat
    13801 tgttttcttc tgctattttc tgcattaact ctaaaggtat tcttctgtca agaacacaaa
    13861 ctccagcagg atttccttta aatagtttag ttgcaaatga atcaactatg tagtattcca
    13921 ttatttaacc tctctttcta acataaaata tacatcaatt tacattcaat aaaactttaa
    13981 atttcttaca tatatttttt aatattactc tgttcaacaa cttttttacc taacaatgta
    14041 attgcttttt tggcacaatt atttacacaa cgataacaca ttgtacattg cccatttgca
    14101 acaatcatac aatttttaat tactagattc ttcattggac ataaattaac acatttacca
    14161 catacaatac attcctcagt attaattttt agtttatctg tatacatatt tgttttattg
    14221 ataaaataca atcgttgtcc aaataaccct acaatatgac tcaaaaaacc caacccttct
    14281 tttggtggtg acccatgttt cagtttttta gcagccttag ttactttctt ttctgctttt
    14341 attacaagct ttttattgta ctctaaagaa tgttttaata ccttttcatc tcctatacta
    14401 tctggcatct tcacatgtaa tccaccaaca gttatagcac cgtatttttt taatattcta
    14461 gataacacac cagagccatc tccactaaat agagccatag ttgcaataat aaaaacacgt
    14521 ttaccattcc acaaagttga atgatttatt ataaaatctt tcaatatttt tggcacatta
    14581 ctatattgca caggataact aaaaataata ttctcatgat tattgatgtg ctgtaaaagt
    14641 tcattatctt caatagaata agaatttgct gttatatcat attcttgtaa aaatttttct
    14701 acacagtatc ttgaatttcc tgttccacta aaatatattc ctatcatagt acttctccta
    14761 atagctaaga ttatatagct ctattcttaa aattctgact ttttatatta tctaaattaa
    14821 gtagtttatt taaataatat aaacttaatt caacaagtta cctactgaac ctcccatgac
    14881 taaagtcata gggttcctgc ttgatagaat tttgcataat caatttacta aatacttgtc
    14941 taattctttt tttatcattt taaatataca ttttaataat attagtaagc tatacttcct
    15001 taagcatata ttttccctaa agtaaaacta ttgatgatta ttccattata taacctaatt
    15061 ataaatttag taagttcatc acatactata cccttaattc tcaattataa agttccctaa
    15121 aaaatatttg aaaatttaac tataaaaaaa ttccctattg cctaatatgt ataagtaagg
    15181 agtactatat agtactcctt atattgtact cttataacat tgatgcgtta atattatttt
    15241 aatcttaatc attattgatt tgatttaaaa ttataaatag gttccaatat tttaattatg
    15301 tcaactatat ctcttatagt tttaataatc tcatcaattg gtttatgtgc ttgttgcact
    15361 ttatcaatag tattaattaa atccatttac acatctagca cctgtatggc aatttagcat
    15421 tatccttatc ttatactttg ttgtaaattc ttgattacat aattctatta tctaacttat
    15481 agctgtgcaa tctacattat ctgtaaatat ctttgcttta tatattttcc ctgaacctct
    15541 aacattaata taacagcctt tttgctatac acttttaact atatacttaa ttttaataac
    15601 attacttgtt tataaaaata tactatagat aaatttcaca taaaaataat aattcaagta
    15661 ttgattttta tataagcaaa tttgtaatgt actaaaaata acataaaagt ttccattttt
    15721 caatatgtaa gcttttatta tgttatttta ttgttaaagg tttcaagttt ttatttattc
    15781 attaaaaaaa aattattatt tttgtaaaac taatcctata gtcatactat cgtcagaata
    15841 tgcttcaccc ttaacattac taaaaaactc aacagactta aatccaatat tttcaacttc
    15901 taataagaaa ctttcttttg aaaaatagtg attccaaaca taaaacacat tttcatcttt
    15961 ttcggttatt acaagagttt gttctaatgt attataatca ttgtatttac aattatcttg
    16021 tagacacata tatttttcat tactccaaaa tcctccatct tcatttattt cccaatattt
    16081 aatttcctca aaattattgt atttatttat tgtaaataca tctaatatta attttcctcc
    16141 aggttttaaa ctatcataaa ttttttccat taataacctt ctattttcag ttgacaaagc
    16201 tccataatca caataaatta aagtagccaa atcaaattcc tcattatagt tcatatttaa
    16261 ataactttga aacaagtaat ttatgtttaa attagtctcc ttatttctat tttgtgcata
    16321 gttaatagaa cgttttgaga aatcaattcc agttacctta taaccttttt gtgcaaattt
    16381 ctctgcatat aaacctggac cacaacctaa atcaagtaat ttaggataat tagctggatt
    16441 tgcaactgta actatccaat ttacagaatc ctcgataaag ttaaaatttc tacttgctcc
    16501 ttcaaaatta gtgtctaagt gtgcttttaa taattgcttt gaaatatact catcatccca
    16561 aaaattaatc tcactttgtt tataaagctc tggtctttct aaatacatac ataatttgtt
    16621 taacaaaaca taccactcct taaaatttat ttttaaaata aaaaaaccac agagtttact
    16681 ctgtggttta acttcaaaaa gatgagttta aataagagta aaatagaacc aaatattttt
    16741 aagcacaaca ataaaactat agctttactt tgtttgctta ttaaatttga ttttattcac
    16801 tcttttccag caatacttat atcaacgatt ttatgatatc aaatatcacc gaacaacatc
    16861 atcctttcat atttttattt tatgaattaa ttatatattt aaattttata tttgtcaata
    16921 aattttaatt ttaataaatt ttaatcatca tagtaaaaaa aattttttta tataaaacta
    16981 atcaaaaaaa gatatctgta catctcctga gttttttaac ttttccttca ttattttaga
    17041 gtaactgctt gatagagaat acttatatct aagttcatta accattttat acaattgatt
    17101 tttatactcc ctattaagtg aaccttcact atatagtatt gataatttat caaataattc
    17161 aggaaactct tttttaacaa agtcaaaaaa aacacctcta gttatacccc ttaagtataa
    17221 agtcccaggt aaaacataat gtacattaca ttcttttgca tgtctaaata gcgaatttat
    17281 attatcaaaa tcatctgtaa tatatggtat aattggcata atatgtagac caactgatgc
    17341 gttagttttt ctaaattctt ttaacatttt aaatcgtctc attgaatcca caccatttgg
    17401 ctcaataagc ctttgtgtat cttcatttac tgtggtaatt gtagatgcta cattgatata
    17461 tgtaatccta gatagcttat caattaaatc gtaatctctt aaaattaaat ctgactttgt
    17521 agaaataata gctggtgtct tgtattttat cagcaggttt aatatttctg gcataatttt
    17581 atagtcagct tctattggtt gataactatc tgtaactcct cctatattga ttacttcttt
    17641 cttccattta ctgcttttaa gttgtctctc aagtttttct actatatttg tttttacata
    17701 aatctcttca aaataattat tggagttgat gtacttatga gagtaaatag cataacaata
    17761 tttacatcca tgctcacatc cacgataaat atttaaatcc catttatatg gcatactcct
    17821 ttttaattta ttacaagcta tttcacaatt tattggaata tattttattt tattatacaa
    17881 ataatcacct ctttatatac aataatacta taatgttaat ctatagtaat taaaacatga
    17941 tatttccaga ccacaattca taatctttac tatatttaca aagtaatata ttccattcat
    18001 ttattggtat tcctccacat gttgcttttt cttctaaatt atataagcat tgctttaaat
    18061 acattttatt atgccaatca tcatataaat tttctaaatt taaactatgt tcattagata
    18121 tatcatttgc aaaatttcta tttttccatt ttaatatact tgtatattta agttcattag
    18181 gtttataatt tctttttaac atttcatcat gtacaatttt tgtataaaat ttaaattcat
    18241 ctatactata attcaatact ttattcacta atagatggtt tggagtacct tttttatcta
    18301 tagaaccttt tatagcaagt agctctctcc attgacttac taattgattt ttaggtaata
    18361 catcaattaa atccttatgc cataatctca taatttaact ccttactata aaatatttta
    18421 taaattcaat aaccaatcta tcttacaatt ttacatgttc aataatttta tctgtaaaaa
    18481 ctataccatt taaatggtct atctcatgac aaagacattt tgccataaaa cctgaacctt
    18541 taatttctat tttctcaccg tattcgttta gagcttgcac tgtaactttt tttggtcttt
    18601 ttaatttacc ccctacttca ggaaaactta aacatccttc aacaacaatt tgttcccctt
    18661 cttgtttaat aatttcagga ttaacaagct taataagtcc ttctcctaaa tctataacaa
    18721 ctaatcgctt taatactcct acttggcatg cagctaatcc tcctccattt ggagtattgt
    18781 acatggtctc tgccatgtca tttaatattt ctcttatctt attatctacg ttttctacat
    18841 acttactctt ttttcttaaa atttcatcat caaatgttct tatctttctt atagccataa
    18901 tattctcccc tacttatctg tgctcttgat tttctttcac tttcttcaaa taaaaataca
    18961 ctttcaattg gttcattaaa aattcttgaa atatcatatg ccaaccatat tgaaggttca
    19021 tttttaccag tttctatagc atttatagcc tgtctagatg ttccaactaa tcttcctagt
    19081 tgttcttgtg tcaatcctaa tttttctctt aatatcttta ttttattttt catatttgct
    19141 ccattcatgt aaggttaact ttacatctta aatgtaacat ttttcaacat tactgtcaag
    19201 tcaaccttac atttatatat taaattttct tatttaacta tttttttact aaacttttta
    19261 aataataata aaatccatat taatgatata acaattgaaa taaacatata gacataatta
    19321 tcttccttaa atgccttcga tatccaaaaa gttggtaaga agaacagaat atattgtgct
    19381 ttatttaaaa taaaaaatgg tgctaaaata cccatcataa aaacacctga gaatttcgta
    19441 attaccattc cttctagttt atttgatgat aaagaaatca ctaatattga tataataatt
    19501 ccttgtaata aaactaataa agaaattgat aagtttaaca agagagaaat tttagttaga
    19561 gaaaaaaaga taagtagaat catagtaata ataaatgcta agatacctgg tattccaaat
    19621 cgtgaaataa gataaccagt tttaccaagc ggagttacta ttaaatatct tgaaatgctg
    19681 tcatcaattt ctcccaatat gaccattgta gaagcaaaaa agaacataaa aggtgtaata
    19741 aaagctaaaa gtaaatcaaa catcaagaaa tacggctcta aatttaattg ataataaaac
    19801 ttgttaagtg atatattctg tattaatggt attccaaatt ttataaatac tccacataat
    19861 attggagaaa cacatactat aaaaagcata gcatctctct taatttgttc aaactcttgt
    19921 ttaaacgcat ttataatctt tatcatagtt ttactccacc tacctttttc caactgctac
    19981 aaataaaata ataagttata aaataaatta ttattataat tgatattaaa attaaaatat
    20041 taatcattat aaaattttta tctccactaa taagagaaat gcatatatta aaagggtaca
    20101 aatttgcata tgattttgtg tcaagaagta cattcagtat tggtggaata aagcatataa
    20161 tctcaatagg aatacttaaa acaataaact gattcaaact agatgcttta cttgcaatta
    20221 tcaatccaag caaagaaaat ataatagaag aaaaaaaggt acctatagtt gatattataa
    20281 tattatctaa atttaatgta atagcaataa acatagctac aatactagat attactccta
    20341 atgaaattac ctttgataaa atatactcag atacttttac tggagataca gctaaggaat
    20401 ttaaaaccct ttgacttttc tcaagaagta caatagctcc cataaaaaac aatcccattg
    20461 ctgctgggtc agaataaatc ataattatag ctattttttc tctcataaaa gttggaaata
    20521 cgttgataat acaaatatat ataatactta aaaaaagata taaaaaataa aaaccatact
    20581 taaattgaaa ctttatatct cctaaaataa gattttttag cctcattgta aattcctccc
    20641 tgttatctca ataaaaatat catttaatgt tggttcactg ctatgtattg ataaaagttt
    20701 atttttctca ataagcatat tcaaattttt atcattagca gtattgttca gaaaacattc
    20761 agaagttttt tctccattat caaagtaagt atatctaact ttaatagctc cttttgacat
    20821 aataagatta tgtggtgtat ctaaagcaga tattttacca tttacaatga acgctactct
    20881 gtcacaaagc tctgttgcat ctaacatgtt atgagttgtt aaaataatag ttttaccttt
    20941 agatttttct gataaaatta tatctttcat aaccttgcta tttgatgggt caagaccact
    21001 tgttggttca tctaaaaata aaatatctgg attatgtaat aaagctttta taaaatttaa
    21061 tcgagatttc ataccttttg aatactcaga taccctttta tttgcttcat tttctagtcc
    21121 tactgatttt aataactcat caatagatag tagtttttta gaatatagag acccaaaata
    21181 ttttaaattt tctattgcag tcaacttttc atataaacta ggaaattcaa aatcaactcc
    21241 tatattttca taaaatttat ttgagtgtct tttactctct actccattta caatgacact
    21301 tcctccataa ttagttatca ttccaattag aatcttttgt aatgtagatt ttcctgcacc
    21361 tgatggtcct aaaaatccta gtatttcccc ttttcctact ccaaagttaa tattttgtaa
    21421 aaaatctcta tctgtataac taaatgataa atcatcaact ttaatcattc aatatcaccc
    21481 tttcaaattt ttgactattt tgactatatg gtcaataaaa tataattttt ttgtttgtta
    21541 ataattttta ataaaagtta ttagcaaaca aaatttaaat tctattccat aagttgtata
    21601 actaatccgt ttatcattaa tcttaaagca tcttcaaaac aattacttcc aacctcgcgc
    21661 ttatatagca tggtaagaaa aactgctcta aatgctccac taaagctttc tatgtcttta
    21721 ttttttgcat ttggtatgca agatataatc tctccaattt caaagtcatc atgtctaaag
    21781 tgttcttcaa ttatagaatc tggtaacttt ctcaccaata tctcaaattc tccatttatc
    21841 atgagattta aaattgaagt actatcaacc atcctataga atttcataaa tatatctgtc
    21901 aattgttcac atgtaatact ttcatctact ctatttatct catataagat ttctttttgt
    21961 atagagtcat gtatatctct taatacctca aaaaagagca attcttttga ttgaaaaaat
    22021 agataaaaag ttccttttgg tatttttacc cgttttacaa gctcatcaac agtagttttt
    22081 ctaataccat attgctccat acacaatctt gcttcttctt taagtctttt tattatatac
    22141 tctctttctt tatcagaata tgatttaggc atcttttccc tccagtttga ccaaaataat
    22201 ttatatagtc aatataatac tttttctatc taatgtcaat agtattatta gtatatttaa
    22261 aaataagctt aaaaactttc gaaaattctg aattttatat agcatttttt attatctagt
    22321 gttataatta aaatataaat aatatattat tctaattttc atatctattt gcttattttt
    22381 taacttagtt tctaatagaa gtaatcatat ttatcttttt aaaagtttca actttaataa
    22441 aaaataaagg agttgatttt atggctacta ctacaaaaaa ccgtttttac gattatgttc
    22501 aagaaaactt aaataaatat gttgaagtac attcttactt tagcagttgt cctctatatg
    22561 gtgaaattat agaagcagat agcttatcaa ttacactttg ctcaagatat atagatgaat
    22621 ctaatccaac tttaaatgaa acttacacat tatatttacc attaagttca ataatatcta
    22681 ttagaacact ataaaaagtt aattaaatat gggttattta tatat
//
LOCUS       DACXMZ010000043        22518 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.043, whole genome shotgun sequence.
ACCESSION   DACXMZ010000043 DACXMZ010000000
VERSION     DACXMZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.043"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            29..1309
                     /locus_tag="JD016_06505"
     CDS             29..1309
                     /locus_tag="JD016_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAU4999286.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRMLAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1572..2963)
                     /locus_tag="JD016_06510"
     CDS             complement(1572..2963)
                     /locus_tag="JD016_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAU4999287.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(2974..4194)
                     /locus_tag="JD016_06515"
     CDS             complement(2974..4194)
                     /locus_tag="JD016_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAU4999288.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4208..5005)
                     /locus_tag="JD016_06520"
     CDS             complement(4208..5005)
                     /locus_tag="JD016_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAU4999289.1"
                     /translation="MYFMEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYA
                     LETALRIKEEKKANLKVLSMGPPQAKKVIEESFMMGADEGVLISDRRFGGADVLATSY
                     TISQGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVV
                     EIDMPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDEN
                     NYGLNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5013..6287)
                     /gene="larA"
                     /locus_tag="JD016_06525"
     CDS             complement(5013..6287)
                     /gene="larA"
                     /locus_tag="JD016_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HAU4999290.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMATSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6405..7118)
                     /locus_tag="JD016_06530"
     CDS             complement(6405..7118)
                     /locus_tag="JD016_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAU4999291.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7243..8502
                     /locus_tag="JD016_06535"
     CDS             7243..8502
                     /locus_tag="JD016_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HAU4999292.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            8764..9336
                     /locus_tag="JD016_06540"
     CDS             8764..9336
                     /locus_tag="JD016_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAU4999293.1"
                     /translation="MKMATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALA
                     LMLEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYN
                     LKSNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKEL
                     LNHSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9537..10742)
                     /locus_tag="JD016_06545"
     CDS             complement(9537..10742)
                     /locus_tag="JD016_06545"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HAU4999294.1"
                     /translation="MKIALLGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(10845..10951)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11043..11411)
                     /locus_tag="JD016_06550"
     CDS             complement(11043..11411)
                     /locus_tag="JD016_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HAU4999295.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11463..12740)
                     /gene="obgE"
                     /locus_tag="JD016_06555"
     CDS             complement(11463..12740)
                     /gene="obgE"
                     /locus_tag="JD016_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAU4999296.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(12851..13141)
                     /locus_tag="JD016_06560"
     CDS             complement(12851..13141)
                     /locus_tag="JD016_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAU4999297.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13155..13472)
                     /locus_tag="JD016_06565"
     CDS             complement(13155..13472)
                     /locus_tag="JD016_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAU4999298.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(13487..13798)
                     /gene="rplU"
                     /locus_tag="JD016_06570"
     CDS             complement(13487..13798)
                     /gene="rplU"
                     /locus_tag="JD016_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAU4999299.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(13899..15320)
                     /locus_tag="JD016_06575"
     CDS             complement(13899..15320)
                     /locus_tag="JD016_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HAU4999300.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKVEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15338..16039)
                     /locus_tag="JD016_06580"
     CDS             complement(15338..16039)
                     /locus_tag="JD016_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HAU4999301.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16041..17885)
                     /locus_tag="JD016_06585"
     CDS             complement(16041..17885)
                     /locus_tag="JD016_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HAU4999302.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLKDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNRDGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            18204..19397
                     /locus_tag="JD016_06590"
     CDS             18204..19397
                     /locus_tag="JD016_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HAU4999303.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(19609..20691)
                     /locus_tag="JD016_06595"
     CDS             complement(19609..20691)
                     /locus_tag="JD016_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAU4999304.1"
                     /translation="MKKIRKLATKLLIAMMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDVMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNQTMIADQEGSILNEVYGNNMKPQNKMYEGVPCSSIDSSINTIKFTSSNSNLDPS
                     KHTVYVIGFKKTQYGMVALAPESKPVSYPFEYDKPFSLDFTGKNRSAAELSKYCKKNN
                     IIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(21255..22046)
                     /locus_tag="JD016_06600"
     CDS             complement(21255..22046)
                     /locus_tag="JD016_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999305.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYESEADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFVFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(22050..22379)
                     /locus_tag="JD016_06605"
     CDS             complement(22050..22379)
                     /locus_tag="JD016_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAU4999306.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
BASE COUNT         7240 a         3993 c         2519 g         8766 t
ORIGIN      
        1 aaataattaa agataaaagg agtattttat gaacatactt ggattaatag ttgaatataa
       61 cccatttcat aatggacaca tatatcattt atctaaatct atagaaaaaa caaatgcaac
      121 acatactgta gcaataatga gtggaaattt tctacaaaga ggcgaaccag ctttatttga
      181 taaatacact agagcaaaaa ttgctgtcga aaatggtgta gacttagtta tagaattacc
      241 aactttattt gcttgtcaaa gtgctgaaat tttttctcat ggtgctataa caattttaaa
      301 ttcactaaac tgcataaatt ctatatgttt tggtagtgaa gaaggaaata cagaaattct
      361 ttataatata gctgaaattt tagcaaatga accaaatgaa tttaagttac ttttaaaaaa
      421 atatttagat gatggtctgt tatttccaac agctagagct aatgcacttt ttgattatat
      481 acatcaagac aaaaaaaata attttataca aaattattca aaggaacaat tatcaaaaac
      541 attaaattca tcaaacaaca tactgggtat agaatacata aaaaacctca tccatttaaa
      601 cagtagtata aaaccattta ctatctccag ggtccaatca gaatataatt ccgaagaaat
      661 tgagagtgat atttgttctg ctactgctat aagaacttca cttaaagaaa atacagacct
      721 ttttcattta aaaaatgtta ttccagaaaa aacttataac atcctaaatg aaaaaataga
      781 taatggtttt ttacctatgt ttgatgactt cttctttgat attttaaaaa caattatact
      841 tagagatatg gacactttaa aagagtattt tgaagtaaat gaaggtatag aaaacagaat
      901 ctataaagat atctttacat ctgaatttct acatgaacta caattagcaa ttaaatccaa
      961 gcgatacact ttaaccaaaa tcaaaagaac tctaaacaat atattgctag gtattaaaaa
     1021 aagcgacatg caacttataa aaaacataga tactatacct tatataagaa tgcttgcttt
     1081 taatgataaa ggtagagaaa ttctaaaaga gataaaaaat aaatctgaaa taaaaataat
     1141 aaacaagttt tcaaatatat cattttcatt agatgatacg atttttaaaa cactcataag
     1201 ttatgatata aaagctacaa atatatataa ttcaatatat tataaaaaca ataaagattt
     1261 agtaaaaggc cctatggatt tttatactca tccaatatat gtaaagtagt tttttaaaat
     1321 aactatattt ataaaaatca ttttattaac tctttattat ataagataca atagtcttac
     1381 tttatttatg tacacatgct tttaggatga caacatcaac ttaaatatta tgttactttc
     1441 taaatttaag taaatattgc catcctaaaa attttacaat ttagcttcag atttaaaagt
     1501 aaagtttaaa atattatatt taaatctaat ttaatatttt ttgttttaga aatattatta
     1561 aattaatatt tctaatatat tttccctgga tttaaaatat ttttagggtc aaaagctaac
     1621 tttatatttt taattaacat catataagca tctgaatttg attcatgtaa gtactctttt
     1681 ttagcatatc ctatgccatg ttcaccagaa acttgaccac taagttctct agatttttta
     1741 tacatatagt caaaagtttc ttttaatctt attttccatg tattatcatc cataccatct
     1801 tttaaaatat atatgtgtag atttccatct cctgcatgac caaaactttt tattcttatt
     1861 tttaatttgt cttgaagctc gtgagtgtat cttataaact ctgctatctt atctcttgga
     1921 acaacgacat cacattcatc catttgtgtc gtagatgcct ttatagcttc taaaaatgca
     1981 cctctggctg accatataga atcgtttctt tcttgtgtat ctgatataaa tacatctaat
     2041 gcaccatttt ctaaacataa attagctact ttttcatatt ctttttctat atcttcagtg
     2101 ctatttccat caaatgtaag taaaagataa gcatctgatg tattatctgg aaacttttta
     2161 cctaaaaatt cttctgctgc caatattaca tctctttcca taaattctat agccgttggt
     2221 attgattttg attttattat tttaggaaca gtttctattg ccattgataa atctggaaat
     2281 ggtataagca aacttatact tttctttggt aaaggaagca attttaatat agctttagta
     2341 actattccta aagtaccttc tgagcccact aataaatcct ttatactata tccagaacta
     2401 tttttaacca cctttccacc tacatttata atttctccat ttggtagtac aacttcaaga
     2461 cctcttacat aatcccttgt aacgccatat tttactgctc tcataccacc tgcattagtg
     2521 tttatattac ctgcaattgt agcacttttt tcacctgggt caggtgggta aaataaatct
     2581 ctatcttgaa catattgacc tattgtcatc aataaaacac ctggttctac tgtcaatgtt
     2641 aaattttcat aatcaacttc aagtatttta ttcattttac atagattaat cattattcct
     2701 ccatttatgg caacagcagc acctacaaga ccagtacctt gacctcttgg tgtgactggt
     2761 atattatttt tatatgcgta tttcattatt ttagatactt gttcagtttc aagtacattt
     2821 actaaaactt cagggtactt ctcaatccct ccaagttcat catgtgaaaa atcttcattt
     2881 atatcacttc caactaatac attttcttct ccacaagtat ctattaaaaa atctatatct
     2941 tttttatcga ttaatttata catttctatc cccctattta gctgctgatg ccatatattt
     3001 ttgattatta tctttgttat tttctatttt ttcaatcaat ttaggaataa tatcataaat
     3061 atcacctatt aaagctagat gtgctacatc aagtataggc gctttatcat ctttatttat
     3121 tgctactata tagtcagcac cctgcatccc tgcaacaaat tgaacagctc cagatacacc
     3181 acaagttatt atcaatttag gttttacagt tctaccacta agacctattt gttttttagc
     3241 atctatccaa cctgcttcta ttacaggtct tgttcctgcc acttgagcgt ttaacttatc
     3301 agctaattta tatatcatct ccatatcttc ttgttttttt attgctctgc ttgccacaac
     3361 tatcacttca gcttcttcca gaccaacttc tttatttttt tcttttacac tcagtacctt
     3421 aattttagat tttagttctt ttttttctac tttacataaa gttatctttc ctgtagtatt
     3481 ctctattttt tctggtgctg aaaatatttt atatcttaca gtggcaaatt gtggtctatt
     3541 attaggtgtg tttatatgag ccattatatt tccaccaaat gctggtctta tttggtctaa
     3601 atcagtattt gattgaatat caagtattgt acaatctgct gtaagacctg ttctaaatct
     3661 agcggcaagt cttggagcta atgacctacc taatgtagtt ccaccaacta atactatcgt
     3721 tggttttatt ttatctataa agttttctat tgcttttgaa tatggttcta tcctaaaatc
     3781 tttaaattca tcttcatcat atacaaatac ttcatccacc ccataagaaa tcaaattact
     3841 gcacttatct tttatgtctt ttcctacaaa tacacaatac acaggttgtt ttatttttga
     3901 tgccatctca cgagctttac ctataagctc gtacgttaca ggatgtatat ttccattatg
     3961 atgttcaaca tatactgcaa ttcccctcca ttcatcttta ttaatttgta ctttactact
     4021 ctctataaat tcaaatgcgc cttttggtcc acttttgaca cacaacttgc acatcttaca
     4081 gccagcactt atttctacta tacctgactt ttcttctata gccccaaaag gacatatttc
     4141 taaaattctc tttaagtcag ttattttatc ttgatttaca actatctttg acatttatta
     4201 ctccccctta caccaacttt aattcctcta atttatttac aagtgcaaaa cttagttcat
     4261 ctgcatttcc tcttacaata tttttatctg tatttggctt tggaggaaat attcttacaa
     4321 cttgagtagg agaaccgtta agtccatagt tattttcatc tttatcttca aagtcatcta
     4381 agctatatac aggtatttct ctatctttag tattaagttt taatttaaat gaaggtagtc
     4441 ttggttgaaa aatgtctttt tctacagtta ttaaacatgg atattcaatc tcacatactt
     4501 gtaaatcatt tggcatatct atttcaacaa ctatactttc atctttaact tcaattaatt
     4561 ttgtaacgtt agtaacatga ggtatatcta aaaattcagc tacttcaggt cccacctgtg
     4621 ctgtatcacc atcagtagtt tgttttccac aaattattag gtctaccttt cccatcttct
     4681 taataccttg tgatatggta taggaagtag ccaatacatc tgctcctcca aatctcctat
     4741 cacttattaa tactccttca tcagctccca tcataaaact ttcttcaatt accttctttg
     4801 cctgaggagg acccatgcta agtactttta gattcgcttt cttctcttcc tttattctaa
     4861 gagctgtttc taaagcatat aggtcatagg gattcatttt tgaatctact ccatctcttt
     4921 ttagtactcc tgtagtttca tcaacttcta ctgaagtcgt tccaggaact tgtttaatac
     4981 atgtcaaaat ttccataaag tacactcctt ttttatttaa ctataactga tactccatca
     5041 ggtataacta ctactttgct ctccttacca actatactag tcgccatttc taaagcttct
     5101 ttaaagttaa atgcatgttt catatgcatt ttttttatca tttcaggatt acacttatct
     5161 gttaccatta tcacagtata tttagataat attctcgcta gtatctgaaa ttcccattgg
     5221 tctggaatcg tatctactct atcgacactt ctaattttat ctaaaagttg ttttggagaa
     5281 tctgcatttg ctacattttc ataaaaagat tgacctccat gaccatcatt acatgctgaa
     5341 atcattatta ttactccacc gtctttacaa gtagcttcag ctgctgtcat tcctttaaca
     5401 gattgataaa tattttggtc tagtggatat ccaccatttg tagttacaac aatatcagat
     5461 tttatagaat ttacttttga taactctgtt acaaatctac atcctttagt atgtgctttt
     5521 tcgacatcac cagcaaagga agcaattatt tttttatctt tatctattac aacattcaat
     5581 atgaatgcta attttgcaac tttagctgca tataccatat cctcatgaat aggattatta
     5641 ttcaattttc cagttcttga attatcacta tctataaagt cactacagtg attgtacatt
     5701 atagtctttg cagatgcgat tcctggaagt acgctttttc tgccacctga aaaacctgca
     5761 aagaaatgag gttctataaa accttctgct atcaaaagtt ctgcctcata tgctagtttg
     5821 tttatatata aatctccacc tgaaggcaat atccctactt tacacataga tttatcatca
     5881 gtagatatat gcataactat atcttcatta tctactattt cttctcccat tttataaatt
     5941 agctcttccc ttgtacttgg cctatgaaat ccagttgcca ctataatctt cacatcaatt
     6001 tctggattaa cattcctaat ccttcttaat ataattggca tagttattct acttggtaca
     6061 ggtcttgtat ggtcacttgt aattatgact acattctttt tccccttaac cagttcttcc
     6121 agagaattag agcctatagg attattcata gattcttcaa ctatctcttc ttgagtctta
     6181 tgttctatat aattatccga ttttgactcc aaaacaccga gaaaacgttc atcaggtatt
     6241 tctaaaatca ttccttgttt tgaatacggt acttttaact ttaccatttt taatctccca
     6301 ttataataaa ctataaaatt ggtatgtggt cataccagtt ttacatttat atgatagtac
     6361 cattatctac catttgcaag aataatttat tttataacaa acttttaatt ttcatttttt
     6421 ctataatttt tacttataaa atccatatgt tcctttgctt tctcacatgc aagttctaca
     6481 tcattttcta caactgcatt gtatatagct tcatgctgtt taagcaatat attttcatta
     6541 aaaggttctg cgattatttt ttctcttgca tcttctataa atccatcaaa taattttgat
     6601 gctgtcataa aaagactttg aattaaataa tttttactta atgttgctag tttactgtga
     6661 aatttttggt ctaataatac cagtactcga ttattatctt ttttattagt ttctaccctc
     6721 atttcatcta ttatatgttt caattctata acttcagaag aagttattct ctttgaagca
     6781 agttttgcaa tctcaatttc taggattatt ctaagctcta atatatcttc aagtgtacca
     6841 ttattaagtt taaaaatcat agataaaggt tctattaaag ttttttctat atttgtacat
     6901 ataaaatttc cttcaccctg tctgctttct atcactccca tagtctctaa taccctaata
     6961 gcttccctta tggaagttct actcacattc atttgttcag acagttctct ttctgatagt
     7021 aatttatccc ccttttttaa ctctccattt agtattttat attgtatttg ctcaattact
     7081 tgttcataaa cttttttact agctatacta ttaaacattt tttcctccaa tatgataatc
     7141 gttgttgtaa tatatatcta ttatatatct taagccatta cattcatata tttttattac
     7201 ttttaatata atatcttata actcaaattt acaggtgata ttatgaaaaa aaataaaatt
     7261 attgcttttt ttgctccatc tatcatagtt ggagttctag ttttagggat tatcctatac
     7321 ccaaatgaag ctataaaatc tgcaagagaa ggcttttcta tatggtctaa tgttttagtt
     7381 ccatcactac taccatttat aattggagca aacttaattg ttgatttaaa aatagtggat
     7441 attatagggt ttatcatcaa tcccataact agatttgtat ttaatgtttc aggaaagagt
     7501 gctttagctt ttgctatatc tacagtatct ggttatccag taggagtaag tttagcttct
     7561 gaatttagga gtaatggaca aatttctaag tatgaagcac aaagacttgt gtctttttgt
     7621 tctacatcag gacctttatt cataataggt tcggttggta caggtatgtt ccaaaattca
     7681 aacttaggtt atttaatgat tctctgccat tatctaggaa ctatattggt tggtcttttc
     7741 tttagaaatt atggtaatga aaatcttcca aagacaaaat tggatttaaa aacaaatata
     7801 aaaaatgtaa tcaacattag aaattcaagt gggaatggtt tttttgtttt atttggaaat
     7861 gctgtattta atggagtaaa tacattactt acagttggtg gatttgtaat tgttttttca
     7921 gtagtattta aaatactatc tctttttaat gtaatctcat taatagcatc agttatatat
     7981 ttgcctcttt catttttagg tgttagtaaa gaactctgtc aagcatttgt aagtggtcta
     8041 tttgaaatta ctataggatg caataaagta tctagtataa caagttctcc agaaatttta
     8101 agagcatctt tagctagttt tttgatagga tttagtggat tatccatcct agctcaatgt
     8161 tgtacatttc tatcaaaaac ggacatagat ttaaaattat acatactaag taaatttgcg
     8221 catggagttt tagctgctat ctttatattt atactttatc caattgcaaa ctctgctgtg
     8281 ttggtttcta gctttgctga tacatacaac gttatatata ataatttaat ctggttttat
     8341 tatcttagct attatgatac aattcttcaa attgtagtaa taatttatct aatctcagcc
     8401 gtatttattg caaaaaaaca tatgaaaact ttatctacaa atgggaaact aatgcgatat
     8461 aaaaatactt ttttcactaa gaaattttca cgaaaaaaat aaaactaata aaacttaact
     8521 aaatacttcc atttttttat tatatttgtc ataattttaa taaccttttt aattagttta
     8581 acagcatagt tatttcatca caatttaata attttattag aaatattttt caaaaaattc
     8641 tccttaatca catatatcac acttgtataa gtttactgga aaatttttcc attatataat
     8701 atttcattta tattatataa taaataccga cctaaactta ccaaatcaaa ctaagaggag
     8761 tggttgaaaa tggcaacaag acctatagaa atagacgaat ataaaaagat aatggaacta
     8821 ttacatactg gctttactta tagtgaaaat ggcgttgaaa aaagatttag aaaaaatcct
     8881 aaagttgcat tggcactaat gttagaagct aatttaggtc taagaatatc agatatttta
     8941 agattaaaga taggtaattt taaaggtaat atgttggaaa taaatgaaaa aaagacgggc
     9001 aaattacagt atagaccagt taataaaaat ataattgagt ccataaatga acatgcaaga
     9061 aagtataatc ttaaatcaaa tgactactta gtaaatataa agacaaaggc tattcaaaaa
     9121 cagttgagga taatttgtaa acacttaaat ctgtataata taagtacaca tagctttaga
     9181 aagctctatg ctactactca atttgaaaag agtaacaata atttagagtt ggttaaagaa
     9241 cttttaaatc acagctctgt agccactaca cagcgatata taagggtaac tcagcaagct
     9301 attaatgaag ctagtgaaaa tttctttatt ggttaattat aacgtataca tttttctatt
     9361 atcttattaa ttcaccagat attaaaaaag agtgtctcaa aatgaacttt ttagttcatg
     9421 agacactctt tttttataaa atcaaatata tttttattat ttcaacactg gaaaaaaggc
     9481 ttatctctct attttgagac accctattaa attttcataa ttcttgcata aacatattaa
     9541 tctatcttca ataatttttt agctgattca ctgtaattat ttctttttac aatttccaaa
     9601 actctatcaa tctcatgttc tgactttgca cttacaaaaa tcgccaattc ttcatcatct
     9661 ataatatcat aactatctac atatttatct aaacattcta aaattccatc tttattatta
     9721 tacccaccaa atctaactac tgcactacac tcatcttcta taagtccact taaatccaat
     9781 ttcccttttg aaaaagactt tatatttgaa ctcctattat ctattatatc tataatatct
     9841 gcataaacag cacttcctgt tgcacctctt ccagctccct tacctacaaa agaaagttca
     9901 cctactgaat cacctactaa aataactacg ttgaactcat tatctatctt tgaaagcata
     9961 ttattattgt ccactaatgc tggtctaaca aatccactta cttctcctga ttcatactta
    10021 cttctagcaa ccaattttat cttacaattt aatttttttg catattctat atctttttca
    10081 tctattgaag ttattccttc taataataaa tctttccaat atactatttt tccataagat
    10141 aaagtagaca atatagataa ctttctagca ctatcatatc ctaaaacatc tgcatctgga
    10201 ttaacttctg caaatcccaa ctcttgtgcc tgttttaaag cgacatcata cggtaaattt
    10261 tcatcataca tctttgataa tataaaatta caagtcccat ttaaaatagc gtttatgctc
    10321 tttatattgt tgccttccag tgattctatc aatggtttta aaactggaat accacctgcc
    10381 acagatgctt caaacttgat gctaacatta tttaagttag ctaattctat aaggtcacta
    10441 cctttttcag ccagtaaatc tttattagct gtcactacat gtattttttt atttaacgct
    10501 cttgttacat aatcaaaagc tggatttaat cctccaatta cttcaactag tatatcaata
    10561 tccttgtcaa atatatcttc tatatcagta gttatcttat caaaatactt cttatacttg
    10621 tgtttttctt tatttttaac taaaattcca acaacttcta tatctctttt tgaagtatta
    10681 ttttctatta aatcaattaa tccacttcct actgttccat accctaataa tgctatcttc
    10741 actgtaacat acccccatca ttttgattat attaaattat ttatatatat aaatatacca
    10801 ataaaaaaag cttgagtata aactcaagcc atatgacggg tttatactca tctttcagca
    10861 taaatgctgc aggatttagc accaatgtat aattaaatat actggttgcc gggcttcaca
    10921 gggccagtcc ctctgccact cttgataaga ttgctaattt aaatttatgt ttaatagtat
    10981 aatacaattt cttccaattg tcactatttt tttaacttat ttccctaaaa ggatataaac
    11041 tattatctaa cagctctttt agttggttta cccttttttg taacattatt atttttagtc
    11101 tttgctttcg cctgttcatg tcctggaaaa agtattttag gctcttcaat attcctcttg
    11161 taaatagtaa atttaaaacc aatagcctgc acaaattcag ctcttaaagc ttcacatact
    11221 gcattagcag tttctttagt atcaagacct gcattttcta atatagtaac ttttactaat
    11281 tctctagttc ttaacatacc atctaattgc tctaagaaac tctctgtaac accttcttta
    11341 ccaatttgtg tagtaggctt taaagtattt gccaaagatc ttaaatacgc cctttgtttt
    11401 ccttttaaca tattctcacc tctataattc tatacttaaa ttatctataa tctatattta
    11461 aattaattgt aaaactcaaa ttctaattca taaattttaa ccacatctcc atcttctatt
    11521 cccatttctc taagcctatc aaaaacacct tgactctcca tagctttttg gaaatattga
    11581 agggattcca tatcatcaaa atttacagaa tacattattc ttctaagtgc tttacctgta
    11641 actacataca caccatcttc aatatctata cttaactctt cttcagtacc aatatcaagt
    11701 tctggtctat acatttcttc ttctgaaact agctccacat cttctacttc ttttagcatt
    11761 ttagaaacat aggctattac ttcatctatg ccttgacgag ttgctgctga cattttaaat
    11821 actgtatatc ctctgccttc taattcactc ttaaacttct caaacttact ctcatcttct
    11881 aatatatcaa atttatttgc aactacaact tgtggtctct ttgaaagctt ttcattgtac
    11941 aatttaagct catcatttat cttgtcaaaa tcttcaatag ggtctcttcc ctctaatcca
    12001 gatatatcta caatatgtat caataccttt gttctctcaa catgtcttaa gaactcatga
    12061 cctaatccta tgccttcaga agcaccctct ataattccag gtatatctgc aagtacaaaa
    12121 ctatctccaa acttagtttg aacaacacct agatttggtg taagtgttgt aaaatgatag
    12181 tttgcaattt tgggtttagc tttagttaca actgatagaa atgttgattt acctacattt
    12241 ggaaatccta aaagaccaac atctgctatc attttaagtt caagagttat ccatctttct
    12301 tctccatctg taccagattt tgcaaaagct ggagcttgtc ttactgcatt tgcaaagtgt
    12361 tggttacctt taccacctat accacctttt gcaactatag ctgtatctcc ttcttttttt
    12421 aaatctgcta atacaagacc tgtagcctca tctcttatta ctgtacctgc tggtactttt
    12481 aatactaaat tttctccatt tttaccagct ctttttcctt tgcttccgtc gccaccattt
    12541 tgagcttgat attttttctg gtacttaaag tccattagag ttctaagccc caaatcaact
    12601 tcaaatatga tgcttgctcc acgaccacca tctccaccgt ctggaccacc agctggaacg
    12661 tatttttctc ttctaaatgc taccgaacca tttccaccat ttccagcctt aacaaatatt
    12721 cttgccttat ctataaacaa attcttcacc tacttaatta aaaactttta attagcatta
    12781 ccaaaaatac cattataaat tcatcagtat tataagaaaa aggagtgcct caacactcct
    12841 tttatgtata ttactcagct attgctactg gatatatact aacttttttt ctcttcttgt
    12901 cttttctttc gaatttaaca gtaccatcaa ctaatgcaaa taaagtatca tcactacctt
    12961 ttccaacatt agttcctgga tgtattttag ttcctctttg tctaactatt atacttccag
    13021 cagttactaa ttgaccatca aatctcttaa cacctaatct tttagatata gaatctctac
    13081 catttttaga agaacctacc ccttttttag atgctaataa ttgtaaattc atgtttaaca
    13141 tatggagtca ccccctactt ttttaaaatt tttatatttt ttgggtattc ccgtttaatt
    13201 tctttaaccg ctaattgaaa gtgatttagt aataattgtg cgtcttttac atgtgtatct
    13261 ttgactatac actctatatg accttcttct cctactactt tttcaaattc aatcttctgt
    13321 aatttgtcta aagaatttat aactgcaata gcattagatg taactgcagc acatactata
    13381 tcatagccta tttcagcaaa atcagcatgt ccctttaaac aaaaaccttg caacaagaaa
    13441 tcatcattat aataatattt aacttttatc ataacttaac caataactaa gcgtttattt
    13501 tttctacaac tatcttagtg tatgattgac gatgtccttg ttttcttctg taatcttttt
    13561 ttgctttata cttgaaaact ataactttct tagctttacc ttgttcaaca actttagctt
    13621 gaacagatgc tccttctact actggagaac ctaattttaa ttctccatct ttagagcaag
    13681 ctaatacttc gtttaaagta acaacatcac ctgcgttagc ttctaatttt tcaacgaata
    13741 atacatcacc ttcagaaact ttatattgct ttccaccagt ctttactata gcgtacatta
    13801 aatacacctc ctcggcctca aactcgccag tacaaggtac ttcatttgaa gatttaaacc
    13861 tttactgtgc ggcattacaa ttattaatta taaaatattt acattttatt gtcaactatt
    13921 ttatcgaaaa taacattggt tttattgtgc tttaatgtac tatttgcgtt taaagaaatc
    13981 tttatattat atttttcacc aattttatca attatatcca tataatattc ttttatttgt
    14041 tcaaaaataa aatcattaaa ttctaccatt atgtttttgt aaacagtatg ttcacttatt
    14101 ctcataattt ctttttcaat actatctagt atataatgta cagattttat tgtttgttca
    14161 ccatcacaac atggacaaga cattaaataa tacttatcaa tggattcttt ttctctacgc
    14221 ctagcaactt ctactaaacc aagttttgtc atacctaata cttcaacttt tcttttatct
    14281 ttattaaatt cttcttctag gatgtgtaaa atctcctttt gatgcttctt tttatgcata
    14341 tcaataaaat cgattattat tatcccagct atgtctctaa ttctaagctg tcttacaatt
    14401 tcttttgcag cttcaagatt agttttataa acggtttcat caagtttacc agttccaata
    14461 aacttacccg tatttacatc tataaccgta agagcttctg ttttttcaat tattaaataa
    14521 cctccactct ttaaccatac ttttttattt aataattttt ctattttaga ttctattctg
    14581 tataaatcaa atacatctct attttcctca agacacagtt tatcaacata agtcttatct
    14641 atgcctctta aaatagactt aagttcagag tacttatcat aactattggt aattatagat
    14701 tcaatatcat cattcacatt atcttttaca tatttagttg caaaatctaa agacttatat
    14761 aataatttcg gacctatacc aagtttatat tcttttaaaa tgttttcata ctttaatttc
    14821 aactcttcta tatctttctt tatttcatca tgagtgcaac cttgagcctc agtccttatt
    14881 attaatccta gattttcctt atttactgcc ttagtaattt tttttaatct aaatcgttct
    14941 ttttcatcag taattttatt tgatattgta gttctatcat tggaaggtat atagacaata
    15001 taccttccag aaatactaat ctcagtgttt aattttgcgc ctttagttcc tatttcttct
    15061 ttatttatct gaaccaaaat atcctgacca gattttatag tacttccctt ttttaattgt
    15121 aaataagcaa gtttcttaaa tccaatatct acaaaacaag cttctattcc tggtataact
    15181 ttttttacga ttccacgata tatattggat acagtcttct tatttaaatt gtcttcaaca
    15241 aaaagttctg ttaatctttc atcttcaagt actgctgttt tctgggaccc tattagggac
    15301 tcaataacta tctttttcac aacttcacct ctcgagctta cataggagtc actaattccc
    15361 catcttctaa tacatataaa tctcttctca atatatcaac atctaatggg tcaatatcaa
    15421 gttcaaacat ctctaatatc tttggtataa gtatattagt atttaaatta gtttttgagc
    15481 ctgtagctat agtagcagta agagtaatat gttcatcttc taaatttaaa acatcaaagg
    15541 ttcttatcat aggtcttata tctacttcaa ccatcttacc ttttttattc ttttttgtta
    15601 tgattatttc tttattattc ataaagtcaa ttacttttct ttttacaaac tctttcgtca
    15661 taggtctttt caaatctata ttaaacaaat attctccata gtctatcaca gaagaaagag
    15721 aaggtgtttg tctatcaatt tcttttgctt tgataaaatc tatcccatct ggaagttgaa
    15781 ttgatacttt atttaaaaat tcctctactg ataaatcatc ttcttctatt tcaatatcta
    15841 catactcacc ttgactttct gtccctaaag ccaaagcatt gccataactc attttagggt
    15901 gaggattaaa tccttgagaa tgtgataagt ttatttcagc tcttctaaac gctctttgta
    15961 acaatctctg aaggtcaaga tgtgatatat atatcatgtc gccttccttt ttaaatttta
    16021 ctcttattat cttactcata ttagcaaagc ccccttccta tatcatgtat atttattcca
    16081 caaccattgc agttatgtct acaatcagaa gtgatttttt cttttttagc attttcgtct
    16141 tctctaatta agaatttttt actaatacct acatctatat ggtcccaagg aaatacctct
    16201 tcatagtttc tatttctatg tgcataaaaa tctatagaaa gattattctt ttccatagct
    16261 tctttccata tatctaaatt aaaatgttct gcccatccat caaatttagc acctagtcta
    16321 aatgcatcat atataacttt tcctattctt ctatcgcctc tagctactac tgcttccatc
    16381 aaactagtct ttgaatcatg ataattgtat tttatattgt tatttcttaa tttatttact
    16441 aaatgtctct gcttattaat aacttcttct gtagtatctt gaccatgcca ttggaaaggt
    16501 gtaaatggct ttggtacaaa tgtagaagta ctcactgtaa cactaaaact tctctttagc
    16561 tttcctccat ttacttttct ataaatatca ataactttat aagctaattt agctatacca
    16621 tctaaatctt cataagtctc agttggtaaa ccaatcataa aatacaactt aacactattc
    16681 caccccatct caaaagctct ttctgttgca ttttccaaat cttcttctgt aacaccttta
    16741 tttataacat ctctaagtct ttgagttcct gcttctggag caaaagtaag tccagatttt
    16801 cttacttgtt gtattttatc agcaatttcc attgaaaaat tatctaatct aagggatgga
    16861 agcgaaatcc caatattatt agatgcatat tcatctacca aataatcagt caactctgat
    16921 aacttactat aatcactcgt actaagtgat gataatgata tttcatcata tccagtattc
    16981 ttaactaatt tatctaatat ttctttaagt cttttaacac tcttttctct tattggtctg
    17041 tatatcatac ctgcttgaca gaatctacat cctctagtac atcctctaaa taattctagt
    17101 actattctat catgaacagt atctatataa ggtactatta gtttttctgg ataatctaca
    17161 gtctcaacat cttttattat ccttttagta ggatttttag taatcccgtc tctattagga
    17221 gttacacttt gaactgtacc atcttcattg tacttgacat cataaaaact tggtatgtat
    17281 actccttcta tgctagatat tttatataaa aaatcttctc tagtcgtttt atttttcttc
    17341 cattctttat actcatttac tacttctaaa ttaacctctt caccttcacc taatattact
    17401 acatctacaa aatctgctaa tggttcggaa ttataagcac aaggaccacc aaccattata
    17461 aatgggtctt tcaaagttct ttcttctttt aatattggta tattggctaa atctaatata
    17521 tttaagatat tagtgtatga aagttcatat tgaagtgtaa aagtgacaaa atcaaagttt
    17581 gttataggct ctcttgactc taatgcaaat aatggtatac ttttttctct cattatattt
    17641 tccatatcaa ctgcaggaga atacactctc tcacaaaata catcttcatc cttatttatt
    17701 acatcatata atatatgact ccctaagtga ctcataccaa cttcatataa atctggaaag
    17761 caatgtgcat atcttatcaa ttcactatta ctagtatctt tatgaattga atttatttca
    17821 tttccaagat atctagcagg cttttctact ttttttaaga tttttttcaa gtctacttta
    17881 ttcatttatt aaaattcctc caaacaagct ctttattttt attcttattt acaagtaata
    17941 ttatacacga taaaatattt tctatcaaaa atataactaa aatattgaat ttaaaataca
    18001 tattattcac aaaatagaat agctaaaaat atttaagaat attttctatt aaatttttat
    18061 tgaaatacaa tttttttaca aagtatgata tattataaac ataaaacgat tgttttacat
    18121 gacttatcat gacatctttt gtttcattca cctcaaaatg gggtatgtta cctatataat
    18181 gataaatttt aggagggtta aaaatgagta aagtttttga agtaaaaaaa gatatctact
    18241 ttactggtgt agtagatgaa ggtttaaaag tattcgacat aataatggaa actgagtttg
    18301 ggacaactta caactcttat ttaatcaagg acgaaaaaac ggttttattt gacactgtaa
    18361 aggcaaattt taaagatgag tttttaagta atttatcaga ggttactgat atagctaaaa
    18421 ttgactatgt tgtaattcat cacacagaac cagaccatgc aggtagttta aaatacttat
    18481 tagatataaa tcctaatata gaagtttatt gtactaaagc tgctaaacta tacttagatg
    18541 gtcaaataaa tagacctttt aactgtcatg taataaaaga tggtgaaatt ttaaatatcg
    18601 gtaaaagaaa tcttagattt ataactgctc cattcttaca ttgggttgat acaatgttta
    18661 cttacattga ggaggacaag actcttttaa cttgtgatgc atttggttgt cactttgcaa
    18721 gtgttgatgc agaggtagtg aatagtgaag attatctaaa atcagctaaa cactactatg
    18781 attgtatagt taagccattt gctaaacatg ttttaagtgc tgtagataag gtggtaggac
    18841 taaacataga atttgatact atattgactt ctcatggtcc aatgcttact aaagacccaa
    18901 tggctgctgt taaaagatat gttgagtggt ctacagaagc tgttaataca actaatcaaa
    18961 atcaagtttc tatattctac ttatcagctt atagtaatac tttagagatg gctaaaaaga
    19021 taaaagaagg acttgataaa gaaggtgcaa aggctgaatt atatgattta gaagatatga
    19081 cattaacaga aatgcacgat acattagtag tgtcaaaagt aatattacta ggctctccaa
    19141 ctataaacaa aactatggtt aaacctatgt gggatttatt ctctgttata gacccaatgg
    19201 ctaaccaagg caagatagca ggagtatttg gttcatttgg ttggagtggt gaaggtataa
    19261 ctatggctga gactttactt aaatctatgt cattcaaaat gcctgtagaa tctcttaaga
    19321 aaaaattctt ccctagtgaa gagacattaa aagagtgtat ggcatttggt gcagaatttg
    19381 caaaattagt taaatagcca ttaatcacac ataatattat ttaaaataaa tgattttata
    19441 tttaaaaata aattaaaatg gagttgtctc aaaattaatt tttctaatct aattttaagt
    19501 tcaactcctt ttttattttc taaaatgact gatataatga taaatgcacc ctttgtgcga
    19561 gggtgcatta tttataatat gttctatttg tacactagta tatttgtatt aattcacata
    19621 tctagcgtat gctaccatcc ctcttccaga attagatgct gtttcaccaa tttccacttt
    19681 aataattata ttattttttt tacagtattt tgataattct gctgcagaac ggttcttccc
    19741 agtaaaatct aaagaaaatg gcttatcata ttcaaaagga taactaacag gtttactctc
    19801 tggtgctaat gcaaccatac catattgtgt tttcttaaat cctattacat aaactgtatg
    19861 ctttgatggg tctaaatttg aattactact tgtaaactta attgtattta ttgatgagtc
    19921 gattgaacta catggaactc cctcatacat tttgttttgt ggcttcatat tatttccata
    19981 aacttcatta agtattgacc cttcttggtc tgctatcata gtttgattcc caggtccaac
    20041 tggcgcagaa ggcttagctg gcgtaggttt cttaacagtt ggcgttggat tctgtgtctt
    20101 tactacttcg ccaacagctg gatatgcttt cttgtcaaat ggtaatgggt gagtaagact
    20161 cttgtatatg atgtctgctc ctgctgattt gttggcatca atatctacat gatacaaacg
    20221 tccatcctca aaacgaataa gattccactg atggtcattt gttgtactat gtacataata
    20281 acattcaatt cccataacat cacataaagc tttcaagctc ctacacattc cagaacaagc
    20341 acctttttta gtaaccaatg tataagcagt attggctccc tctgctgcgt tccagttaac
    20401 atatgttgtg ttgtttttaa tgtgattata agcaacccta atcttagcat ctgtggtcat
    20461 gttctttgta attttcttgt tcataaagtc tgctgttgct tgtgctaccg aattaagttg
    20521 agcttgtgac aactttggac catatatact gttggttcca gctacatagc ttttcggtgt
    20581 ctttgctgta acgtctttag ctgtcatacc ataaactgtt acactcatac atgaggttaa
    20641 aaccatcatt gcaatcaata gtttagtagc taatttcctg atttttttca tattagcatt
    20701 ctcctttata atatattttt actttcaatt ccctaccatt aattaagttc attttagatt
    20761 taaaatatgg attatcaaca tgtgtgcata gcgattcacg tggtcataga atatttagat
    20821 ttcttggaaa atctgttcta tatttcctat atagttcgta tacaatccaa ctcacaaacc
    20881 tgcatttttg atatcatcac aataaaatga taataattaa tatattcaaa aatgttggtc
    20941 tgtaacaggt atgtcaattt ccaattatcg gtaatagcga gagtgaggag aattgtcaat
    21001 aatgcattta aaattaaaat tgtatacact tcatacattc tcccccaaca acattataca
    21061 gattataatt tgtatattca atacattttt tggaaagtat aattatatca aaatatgtga
    21121 ttatgtatag atacaatcca ctatggagaa atataaaaga aaagagtata acaaacaata
    21181 aaaaataaga tgattcaaat tttattttga aacatcttat ttactctaca aattaaaatt
    21241 atttggtgaa ttatttaatt attatttttt attaaagaaa ttttaaatct taatataaaa
    21301 tacgctatta tacataataa tccaaatacg aatgctactg gaattaacat ctccgattca
    21361 aaataaaaga aatattctaa gaaaatatct ggatttacta tattcccatt cttgtctaaa
    21421 aaatcttttc cattatatcc taaatgctgt ataaaattac ttatgttatt ataatctgct
    21481 tcactctcat acaccatatt agcattcaat ccagaagagc ttaaattagt gtttccatct
    21541 ttcatatcta ttacatatag tctaagagct ttgactttac tatacttatc tttatcaagt
    21601 attttttcaa gttctaactt atattcacca atctttgtca tatcatgtct ttctgccaat
    21661 tgctttattt ctccatcaag ttcttttcct aaattataca tattatgttg taaactatta
    21721 ttgtaatacc acataaatcc agaaattaaa aatgcaatta tacttatgta acctaatact
    21781 tttttaaacc ccattacaat ggatttatgc ctatccaatg ataaagatag ttcttttatt
    21841 atattacata attcatactg catttcatca ccatcatcaa atctttcaat tgctttttta
    21901 catgcttctt cttcatctaa cccttctaat ttaaattcat ttgcagattc tataagatgg
    21961 catctcattt cctgttttaa ttctatagta cttttattgt cacatttttt atatagttta
    22021 tctaaatatt catctattag tttcataaat taatccacct ctcccaaaaa ttgattcata
    22081 acctttttaa taaatctcca ttcttgtttc tttatttcta gaaaatcttt accttcatta
    22141 gttaaattat aatattttct tcttccacct gaactttgtt cattgctata ataagattta
    22201 attaaatttt tagattccat tcttttaagt gcaagataca ttgttccttc ttttaattca
    22261 aactcacttc tttctcttac ttgctttgca atttcatatc cataacaatc tttcttttct
    22321 aatatagaaa caattaatat atcgatataa ccttttaaca cttctttatt taactgcatt
    22381 atgtaccacc gccttttcac ctactactct ataatgtata gtacttatat ttactataat
    22441 aatacatagt actctataat gcaatgttta tattctacaa tagacttaaa aataataaat
    22501 agctgtatat taatattt
//
LOCUS       DACXMZ010000044        22476 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.044, whole genome shotgun sequence.
ACCESSION   DACXMZ010000044 DACXMZ010000000
VERSION     DACXMZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.044"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            102..2321
                     /locus_tag="JD016_06610"
     CDS             102..2321
                     /locus_tag="JD016_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAU4999307.1"
                     /translation="MKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRGNYA
                     VLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGDQLI
                     EQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEIIYT
                     VNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLTKVE
                     NSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDLKKF
                     LEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLSEKL
                     SNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVISGQ
                     QPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLDKAE
                     AKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMKKLV
                     SLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALLSTS
                     VHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTSMVF
                     NFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYAIGA
                     TREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLTGH"
     gene            complement(2559..2858)
                     /locus_tag="JD016_06615"
     CDS             complement(2559..2858)
                     /locus_tag="JD016_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAU4999308.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            3064..6105
                     /locus_tag="JD016_06620"
     CDS             3064..6105
                     /locus_tag="JD016_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HAU4999309.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            complement(6266..7540)
                     /locus_tag="JD016_06625"
     CDS             complement(6266..7540)
                     /locus_tag="JD016_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAU4999310.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(7777..11583)
                     /locus_tag="JD016_06630"
     CDS             complement(7777..11583)
                     /locus_tag="JD016_06630"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAU4999311.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLKTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKIEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKEQKIFES
                     GVKYFR"
     gene            complement(11608..12858)
                     /gene="purD"
                     /locus_tag="JD016_06635"
     CDS             complement(11608..12858)
                     /gene="purD"
                     /locus_tag="JD016_06635"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAU4999312.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            complement(12869..14401)
                     /gene="purH"
                     /locus_tag="JD016_06640"
     CDS             complement(12869..14401)
                     /gene="purH"
                     /locus_tag="JD016_06640"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAU4999313.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            complement(14394..14987)
                     /locus_tag="JD016_06645"
     CDS             complement(14394..14987)
                     /locus_tag="JD016_06645"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAU4999314.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGI
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            complement(14981..16057)
                     /locus_tag="JD016_06650"
     CDS             complement(14981..16057)
                     /locus_tag="JD016_06650"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAU4999315.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            complement(16051..17418)
                     /gene="purF"
                     /locus_tag="JD016_06655"
     CDS             complement(16051..17418)
                     /gene="purF"
                     /locus_tag="JD016_06655"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAU4999316.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            complement(17430..18128)
                     /locus_tag="JD016_06660"
     CDS             complement(17430..18128)
                     /locus_tag="JD016_06660"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAU4999317.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            complement(18134..18610)
                     /gene="purE"
                     /locus_tag="JD016_06665"
     CDS             complement(18134..18610)
                     /gene="purE"
                     /locus_tag="JD016_06665"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAU4999318.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            19060..19572
                     /locus_tag="JD016_06670"
     CDS             19060..19572
                     /locus_tag="JD016_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAU4999319.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            complement(19956..21119)
                     /locus_tag="JD016_06675"
     CDS             complement(19956..21119)
                     /locus_tag="JD016_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAU4999320.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNKGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(21116..21514)
                     /gene="tnpA"
                     /locus_tag="JD016_06680"
     CDS             complement(21116..21514)
                     /gene="tnpA"
                     /locus_tag="JD016_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAU4999321.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            21962..22177
                     /locus_tag="JD016_06685"
     CDS             21962..22177
                     /locus_tag="JD016_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999322.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            22183..22476
                     /locus_tag="JD016_06690"
     CDS             22183..22476
                     /locus_tag="JD016_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HAU4999323.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
BASE COUNT         7323 a         3852 c         2748 g         8553 t
ORIGIN      
        1 caaaatttta actttactat ttgtgtaaaa agtaatataa tttaaaattg attataaatc
       61 taactttttt ctttatcaaa atattttagg aggtgagaca tttgaaaaaa actaatagtt
      121 taaaagaaat tttaaactgg aataatatca agaatttaag accttcaaag ggtttaaaaa
      181 atatattaga aatttttaaa agtgacctaa aagatatacg tggcaattat gccgtacttg
      241 taatagtttt aggtcttgct tttttaccat ctctgtatgc ttggttcaat ataaaggcat
      301 cttgggaccc ttatggaagc actgctaata tgcttgtagc agtaacaaat gaagataaag
      361 gaaatgaaat agcaggacaa aagattaatg taggagacca attaattgaa caactcaaag
      421 ataacaagag tttaggttgg cagtttgtag atgaaaaaac tgctcttgaa ggtgttaaaa
      481 aaggtactta ttatgcatca cttaagattc caaaaaattt tacaaaagat ttgacatctt
      541 taattacaga tgatgttaaa aaaggcgaaa ttatctatac tgttaatgaa aaaataaatg
      601 cagttgcacc aaagattaca gataaaggtg cttctacaat acaactacag gttaatcaga
      661 ctatagtaaa aacagttagt gaaatcgttc ttggtatatc aaacggagtt ggtgcaggaa
      721 ttgaacacag tcttccaaaa ttgacaaaag ttgaaaattc ccttattgac cttcaaaaga
      781 gatttgacca tataaataag actgttgatt tagcttctga tgctacttca aaattatctg
      841 atacagttaa agatattaaa tctgatttac ctacaattaa aaagactctg aatgatacaa
      901 aactattatc aagtgactta aaaaaattct tagaggatac aaatgataat ttagacgaac
      961 tttctcctct tattaagtct gacttaaatt taatggttga cctttcatct tctgcttctt
     1021 ctcttacatt aaatttaatt gatgcagtaa atagtggcag tgaagatgta cctaagttga
     1081 tagataattt atcagaaaaa ctttctaatt tgcaatcttt aaacgatact ttggtggact
     1141 ttttaactaa attaaaccaa cttacatcaa ataatagatt ggatgatgta atagataatc
     1201 ttgaagactc tagcaataaa atagattcat ctatatcaac attaaatgat attaaaaaca
     1261 aagttatatc tggacaacaa ccatctataa gtgctttgaa taatgtttta agtctatcaa
     1321 atggtattgg aagaattaat ttaaatattt taaataattt tgactcaaaa atatcaaaac
     1381 ctataaatag tatatttgca aacagtataa aagtagcaaa tgatataatt actgttttag
     1441 ataaggcaga agcaaaactt ccaaaggttg aagaaatact tactacttct ctaaaacttt
     1501 caggaaatgc acaggaaagt atatcattga taagagaaag acttccttta gcaaaaggaa
     1561 tgttggatga tttgattgat actttaagca aaattagtaa tggtgaagat atgaaaaaat
     1621 tagtcagctt gcttgaaagt gatatattga caaagtctaa tttcttaaaa gaacctgttg
     1681 aggtgaaaac agagaaatta tatccaattg aaaattatgg ttctgcaatg acaccatttt
     1741 atagtgttct ttctacatgg gttggtatat tacttatttc agcactattg tctacatctg
     1801 tacatggaaa ctataaacct tatgaaattt actttggaag aggccttact tttttaagta
     1861 tagctcttgt ccaaggtttt ataataggaa ctggagatat attattgtta ggagtaacag
     1921 caaaacatcc aattttattg atagttctgc ttatgtttac tagtatggta tttaacttta
     1981 tagtgtattc tttagtttct gtatttggaa atgtaggaaa agcaattgca atagtactat
     2041 tagtcgttca gattggttct tctggtggta cattccctat acaggttaca cctacattct
     2101 tccaaagaat aaaccctata atgccattta cctatgcaat aggtgctaca agggaagcag
     2161 ttggtggtat atatgctcca aatctaacta aagatatact agttctatta ctatttatga
     2221 ttctgtctat ttgtttaaat gtacttctaa aaggacctat aaatagagtt ataaaaccat
     2281 ttaatgataa attcggcgat agtaatttaa ctggtcatta aaaaattaat tctgtcttta
     2341 aatatatttt tttaataata ttctaagttc atattaaaag gtagtagcta aaaagctact
     2401 acctttttcc aacttaaaaa ctaatattta ctacaaaaac tgtcatagaa aaaaacgaaa
     2461 aaattcgttc tttcctaaat ttatcttata aatatttaga tatattccta cttaataagc
     2521 agagctatac atagtattta atgtataact tacattattt aactcatata atcaattatt
     2581 cctgaattta aattatataa atgctcaaaa ccttcttcag ctaacaaatt acatgcagta
     2641 acacttctat gaccacttct gcaataaact actactttct tatctttata atcgtaaatt
     2701 tcatctatat tattgattaa ctcttgtaaa gatatattta tagcattttc tattttatat
     2761 tccttaaatt catctttagt tcttacatct aataataaaa catccttttc atttttcatc
     2821 atttctttaa gttgcttttt atttatattc ttatacattg tctaaaattc ctccatttta
     2881 tttatatatt acctataccc tttattcttt atacaacaac aaatattatt tttatatttt
     2941 tctctgttat ataaaaagct atacttataa aagctttttt tattgataat aatctgttga
     3001 ttaaaataat taagaatgtt atgctaatta taaattatta aaaagggaaa gaggtaatgt
     3061 aatatgttta agcaaaggct ctctaaatta ttatctagta cattagtatt gagtatgtta
     3121 tttactgctg ctccaaatat tacttttgct gataatacaa aagataattc cgaaaaatat
     3181 caaagttctg atatagaatt acatgattat agtaaaaacg cggaatctta cacaaaaact
     3241 aaagctcttg ctaaagagaa aatccaaact ttgctatcaa agtatggtgc tgtaagtgct
     3301 caatatgctt tgattgataa tggcaaaatt gagatttctg gtaatggtgg agtttacagc
     3361 aaacaggata acaaaaactt aaataaggat aatatgtata gtatcgcatc tatcagtaaa
     3421 atgtttacta caacagctgt tatgaagttg gttgatgatg gaaaattaaa tttagatact
     3481 cctgttgtaa agtatatacc tgaatttaaa atggctgatg atagatacaa ggaaatcact
     3541 ccaagaatgt tattaaatca ttcatcaggg ttgatgggaa gtagttttaa aaacactatt
     3601 ttattagcag acaatgattc ttatggtcat gataatttct taaaagaatt acaaaaacaa
     3661 agattaaaag ctaaaccagg agctttttct gtttattgta atgatggatt tactttagct
     3721 gaaattttag tagaaagagt atctggtatg tcatttacta attttcttga taaatacata
     3781 aataatccac ttaacttaca aaatacaaaa actacagaaa atagttttga tagttcaaaa
     3841 ctagcaaaag catatgtacc ttattgggaa gatgcagttc cacaagataa ccttaatgct
     3901 ataggtgcag gtggtttata ttcaagtgca gaaaatctat gtacatttgc acaaacattt
     3961 atgaaaaatt caaatggtat tctttctcct gcttcagtaa aagctatgga aaacaaagaa
     4021 tatttaaatg gtctatggcc tgaaggagaa gactctatac ttggatatgg tttagggtgg
     4081 gattgtgtca acacttatcc atttaatcag tataatttaa aagctcttac aaaaggtgga
     4141 gactctttat tattccacag taatcttata gttcttccag atgaaaatat ggcagttgca
     4201 gttctttctt ctggtggaag tagtcaactt aatgaaataa ttggacaaga aatcttacta
     4261 tctgcattaa aagaaaaagg taaaattaaa gaaataaaac ctgataaaac atttagtaaa
     4321 ccacaacaag ttaaaatgcc aagctcttta aaagaaaata gtggtcttta tgcttcttca
     4381 aatatgataa aagtagatgt taatgataat ggtactttaa cagtatcttc tccttatata
     4441 gaaaatggac ctgaagataa atacgtatac ataggtcaag atagatttgt atctgaaaaa
     4501 ggtaatagtt gtttaaaatt tgtaaaagaa aaaaataata tcacatattt aaatatgagc
     4561 tcttatgatg atgtccctgg attaggtcag acagcaagtt tatactatgt agctcaaaaa
     4621 gtagatgaca acaatatatc aaatagtgtc aaagaagtgt ggaaaaaaag aagtggcaaa
     4681 ggttactatc tagtggatga aaaatatact tctcaaagct atatgtttgg ctctgtaaaa
     4741 gcaagtttta gtctttctga tgaaactcca ggttatatag ttaacactaa aataatggat
     4801 gagaataact caaatgcttt tatagaaata cctggtgtaa taggtcgtga tttatccgac
     4861 attaaacttc ataaagaaaa tggtacagag tatttatctt ttggaacact aacgtatgtt
     4921 agtgaagatt ctataactaa tttgccagca gaaaaatctt ttacttgtga actagaatcc
     4981 aatggttatg caaaatggta taagattggt gacgatatcg caaataaaaa aatagaagtt
     5041 aatcttcctc aaaattcagc ttttgctgtt tatgatgata aaggtgttcc tgtaaactat
     5101 tctttagtta caaagaacaa tagagttaga ttgccaaaag gaggcgtaat tgttttctta
     5161 ggaagtccaa atgctagatt tgaggttaca tatcaagatg aagtaaatgc aagtgcactt
     5221 acaggaactg atagatatga aacttctatt aagataagtc aagcaggttg ggaaaatgct
     5281 gaaaatgcag ttttaataaa cgattcagca attgcagatg ctttggctgc aacaccattt
     5341 gcatacaaga aaaacgctcc tatattattg actggaagct ctcaaataaa tgaaaaaact
     5401 ttggctgaat taaagagatt aaaagttaaa aatgtatatg tagttggtgg agaggcttct
     5461 ataaatgaaa aatctttaga tactataaaa tcaaacaata tctctgtatc tagaatttca
     5521 ggaagtgata gatatcaaac ttctatgaac attgctaaag aattgaacaa tattagcaat
     5581 atatcaaaga tttcagtagt taatggcgaa aaaggattag ccgatgcagt aagcattgga
     5641 gctgtatcag ctcaaaatga tatgcctata atacttacta atgaaaatag taatataact
     5701 gaaataaata acttgtttaa aaacaaaaag attgataaat cttatgtaat cggtggagaa
     5761 tatacagttt ctaaaaatat agaaagtaaa cttcaaaatc cacagagaat atcaggtagc
     5821 actagaaatg aaactaatgc aaaagtaata aaagaatttt acaaggattc taagatagac
     5881 aatttatatg ttgctaaaaa tggaatgaat aaacaagatg atttaataga cggattatct
     5941 gttggagttt tggctggaaa aactaaatct cctgtaatgc tagtaggaaa ctcactagat
     6001 tataatcaaa aagaactatt taaaactatg agatttaaat ctgtaacaca aattggtgga
     6061 aatggaaatg aaaactcatt taagcaaata aaagaaatag cttaacataa ctaaaaaagg
     6121 ctgtattcaa gtaatattaa ttgaatatag ccttttttta tttaagttat aagttttata
     6181 tgtgtaaaaa ttaaatttaa atattaaatg ataagtttat taagatatag ataagtctaa
     6241 aactttgcct tttttaagtt agtttctatt tatcattcat acttaacaat ttatctaaat
     6301 atactgactt taattgttta ctggctaaaa tctttttagc tggttcaagc tttaatatag
     6361 ctgacagtat agctcccata gctctatgac tagccagagc attattatca aaaattaaat
     6421 tttgtaactg ccctttttca attgccatga gaactgctct atgtactgaa tttgctggag
     6481 ttattatttt ttcatctctc tttaataaca taatgctatt tttatggcaa tttctaacac
     6541 aaactccaca acctagacat ctatcctcat caatttttat atattcttta ccatcttcct
     6601 taaccttgct tatcgcatct attggacatg cagttataca tttaccacac tttacacaat
     6661 tttcatggtt tatatttggt ataaaacttg ttgtctgtac tggatgaaga tttccaaatc
     6721 ttttagctgc taccattgct tcacagcaac atccacagca attacatata aacgtaaccc
     6781 ctttcctaac attttctcca cattgaacta agttatgttc atatgcttga tgaagtagtt
     6841 ctttacattc tattttatcc acacgtcttg caaatttatt atttattaaa gaatttgcta
     6901 cattgtcaaa cgtcatacat atatccatag gagcatcaca agcttttcca acatgttgca
     6961 ttctatgtct acagtaacac atacttatac ctatatgagt tgattcatca attatatgag
     7021 tagccctttc ataatctaaa atactaacct cattatcatt agttaaaact tcctcttgaa
     7081 cgtatactct tccaagcttc gtttctgttg aataaaataa atctttgata aaatcttctt
     7141 ctacattcat atattgatag taaagctctg ccaaaagttt ttggtctata tcgtgtctcg
     7201 ttctcatcat ggcaaactca aaaaaaccag ccattggagg aggcattata tatttcttcc
     7261 ctttattatc ttctatatct agtatcagag ccttactagc aagtttatcc aatactctat
     7321 atgcttcact ttcacttaca ctccaaattt tagctgctgt ttttactctg aaaggcttta
     7381 ttggaagctg agcaactaac ttcgcctctt gttctgtata tagtacattt agtattttgt
     7441 ataatgtatc agatggtgga gcaccttgtg gaaacttgtt tattctttcc tctaaacttt
     7501 tatacatatt cttcgcagtt atatgagaaa tagtaatcac cttctttttc ttttgttttc
     7561 caataaaatc aaatattctt tcttattata tattctctta ctttatataa taccaattct
     7621 ttctaaatag ttaaatacct ctagtaattt attattagat ttcttatata aataaaaaag
     7681 agatgtaata ttaaatgatt atcatcacat tattacatct cttcaaatct aaatatctaa
     7741 aatatatatt ctagctatca attaaacatc aaagttttat ctaaaatact taactccaga
     7801 ttcaaatatc ttctgttctt tttcccctat gatattctta tatacttcct caccaattct
     7861 ttcagaatgt cccattttac caaacactct accatcagca ctagttatac cttcaacagc
     7921 atagaatgaa ccatttggat taaactcaat atcataagta gcttcattat taaagtcaac
     7981 atattgagtt gctacttgtc cattagcaat taattctctc ataacatctt cactggcaac
     8041 aaacttacct tctccatgag aaattgctat attatgaata tctccaacat tagtttgagc
     8101 taaccaagga gatttattag atgaaattct agttctagca atcttagctt gatggcgacc
     8161 aatattattg taagtaagtg ttggagcact ttcacttgga actcttattt ctccatatgg
     8221 aactaaccct aatttaatta atacttggaa tccattacag atacctaaca ttagtccatc
     8281 tttttgagtt aaaaattcat ttatagcttc ttgcactcta ggatttctaa atacagtagc
     8341 tataaattta ccagaaccat ctggctcatc accagcacta aatccacctg gtaacattat
     8401 tatttgagaa gattttattt cgtttactat tgtatctata gaatcttcta tgtctttata
     8461 agttaaattc ttaaatactc ttatacttgc attagctcct gctctttcaa atgctcttgc
     8521 agagtcatat tcacagtttg ttccagggaa tgctggtata aatactcttg gagtagctat
     8581 acttaatgat gattttttag cttctccttg tgatatataa cttatagttt ctatctttga
     8641 ttttacttct tctgttttag ttgggaaaat aggctctaaa gtactacaat gtgctttata
     8701 taattcatct aatgcaagtt cttcaccttt gataaatata ctagcttctt taactgtact
     8761 tccaagcaca acataattgt atccttctaa catacttaaa tcatcatttg ctaattcaag
     8821 tactatattt ccataactag cctcaaataa tttatcatct gtaaatgctt tttcagcctc
     8881 ttcactaaac ttaaatccta ttttattacc aaaagccatt ttacttattg cttcacctac
     8941 acctgcaaat cctaatgcat atgttgataa aactttattt ccatgtataa gttctcttac
     9001 tttatctaag tttctcttta attcctcaaa atctacaaca tcattttctg ccttgtttac
     9061 acataacatt actacttttg agtctgcttt tttaaattct ggagatacta ctttcttcgc
     9121 atctactgtg tcaacagcaa atgaaactaa tgttggtgga acttcaatat ctttaaatgt
     9181 accagacata ctgtcttttc cacctatagc aggtatctca aatttacttt gtgcataaaa
     9241 tgctcctaaa agtgctgaga atggtttacc ccatttttct ggattgttac caagtttttc
     9301 aaaatactct tggaatgtaa gtcttgttgt actataattt cctccaatag caaccaattt
     9361 acacacagat tctactactg catacaaggc accatggaat gggctccatt ttcctacctt
     9421 tggattgtat ccataagtca ttattgtaga agtattagtc tctcctccaa gtactggtat
     9481 cttagcaacc ataccttgtg ttggagtcgc ttgatacttt cctccaaatg gcataagtac
     9541 tgtattacca ccaattgtat tatcaaacat ctctactaat cctttttgag aacaaacatt
     9601 aaggtcagat agtacttttg taaacttgtc tttcatactt gcacacttaa catcttttac
     9661 tatttcatta gagctaaaga atgtattttc ttcatctact ttagtaacat gaacatcagt
     9721 agtttgtttt actccgtttg tatctaaaaa ctctctattt atatctacaa ttgcttttcc
     9781 attccagaac attcttaaat atcctgtatc tgttactgta gctacatgcg tagcttctaa
     9841 gttttcttct actgctaatt gtataaattt atctttattt tcttttttta tggctactgc
     9901 catacgctct tgtgactctg aaattgcaag ctcagttcca tcaagtccat catatttttt
     9961 aggaactaag tctaagttta tatctaagct atcagcaatc tctcctatag caacacaaac
    10021 tccacctgct ccaaaatcat tacatctttt tatcatttga gcaacttctt tatttctaaa
    10081 gaatctttgt atttttcttt cgttaggagc atctcctttt tgaacttctg cactacaagt
    10141 taagattgac tcttcactat gttctttaga tgaaccagtt gctcctccac agccatctct
    10201 tcctgttttt cctccaagaa gaacgattac atctcctgct tcaggtcttt ctcttactac
    10261 attctctttt ggtgctgctg ctataactgc acctatttcc attctctttg caacaaagtt
    10321 ttcatcatat acttctgcaa cttgcccagt tgtaagacct atttggttac catatgaact
    10381 atatccatga gcagcttctg tagttatttt tctttgcata agttttccag gtaaagtatc
    10441 ttctaaagtc gttcttgggt cagcacttcc tgtaacacgc attgcttgat aaacataact
    10501 tcttcctgat aatgggtctc ttatagctcc acctaaacaa gtagctgctc caccaaatgg
    10561 ttctatttca gttggatggt tgtgagtttc atttttaaac attactagat atttttctat
    10621 cttaccatca acttcaacat ctacgtttat actacatgca tttatttctt cactttcatc
    10681 taggtcattt aatttcccat ttttctttaa ttcttttact gcaacagtgg ctatatccat
    10741 taaacatata tctttatgtc tgttcacata tacattgtct cttgagttaa gatacatttg
    10801 atacgcttct tttactatat cattaaactt accatcttct atctttacat tttctatttc
    10861 agtcataaaa gtagtatgtc tacaatgatc agaccaataa gtatctaata cttttatttc
    10921 tgttatagtt ggatttctct tttctgtatc tttaaagtat ttttgaacgt gttgtaagtc
    10981 tcctaaagtc attgctaatc ctttttcact tacaaatgtt tttaatccat tttcatctaa
    11041 atctataaat ccatctaata cttcaactgt tgttggtatt tcagtttcca tttttaacgt
    11101 ttctggtttt tctaaagatg cttctctact atctactgga tttatacagt aatcttttat
    11161 tttagaaaac tcttcatcag ttattttgcc tgataatata taaacttttg ctgtattaat
    11221 agcaggtctt atcccttgat ttacaatttg tacacattga gctgcccaat cacctctttg
    11281 gtcgtattga ccaggaagaa attctattgc aaatactctt tcatcattta attttggtat
    11341 ttcttcataa tataccacat ctaaatttgg ttcagagaat attgtctttg ctgcattttc
    11401 ataaacttct tctgatattc cttctacatc atatctattt aatattctta aattttctat
    11461 attatctatg tgtaaacttt ctactaagtc tttttttaaa gctttagcct caaggtcaaa
    11521 accttctctt ttttcaacta atactcttct aaccattgag tctttatttt cagtattcaa
    11581 cataccttat cctccatttt agaaaaatta tcttcctatg tctgttctat agtgcattcc
    11641 ttcaaagttt atcttcttta tgttttcata aactttttct gctgcatcct taactgtagt
    11701 tgactttgca gttataccaa ttactcttcc tccattggta accaaattac catcaaacat
    11761 cttagtgcca ctatggaata ctactatatc atcatccaag ttttcaagac cagttataat
    11821 tttccctttt tcataactgt ctggataccc tcctgatgta agcataacac atacagcttc
    11881 atcatcacta tagtttattt ctatatcttt taacttatta tctagtatag cttccattat
    11941 ctcatgtaaa tctgtttcta atctaaataa aacagattgt gtctcagggt ctccaaatct
    12001 tacattatat tcaagtactt tttctccatc ttcagttatc ataagaccaa caaataaaat
    12061 acctttaaaa ttaagtccat cttttttgaa accttctaaa gttttttcta atactgtctc
    12121 ttttacttta tttgctaatt cttctgtata tatttcacta ggtgaaaaag ttcccattcc
    12181 tccagtgttt ggaccttgct catagttgtt tactttttta tggtcttttg cacttgccat
    12241 aggaactatt gtatcattgt ctacaaatgc tagaattgaa gtttctattc cttttaaaaa
    12301 ctcttcaatt actattttat ctccagcagc tccaaacttc ttatctgaca tcatttcttt
    12361 taaagttgct atagcatctt ctctattttc aggtattaca acaccttttc cagcagctaa
    12421 tccgtccgct tttattacta ctggatatcc aaaactatct atttctgata tagcttcttc
    12481 taagtttgta tattctttat attttgcagt aggtatgtta tgttttatca taaattcttt
    12541 tgaaaaagct ttacttcctt caagttgagc acatttttta ttaggtccaa atactcttaa
    12601 tccttctttt tcaaactcat ctactattcc cataactagt ggtacttctg gtcctactat
    12661 tgtcagacct atttcatttt ctttggcaaa ttttaaaagt tcttctatat tagtatcacc
    12721 tatatttaca cattctgcta cgtttgcaat tcctgcatta cctggtgcac aataaatttt
    12781 ttctacattt ttttctttgc ttagtttcca gcatatagca tgctctcttc cgccaccgcc
    12841 tacaaccaat atcttcataa tatcctcctt agtgtttgaa gtgtcttatt cctgtaaata
    12901 ccataaccat atcatacttg tcacaagctt ctattgaatc tttatctttt atagagccac
    12961 ctggttgaac tactgctgat attccatatt catgagctaa tgttacacaa tcatcaaatg
    13021 ggaaaaatgc gtctgacgct aaaacagctc ctgtaaaatc tttatctttg ttattttcaa
    13081 gagcattttt taatgcccaa attcttgatg tttgtccaca acctaaagct agagtttgtc
    13141 cattttttac tattgcaatt gcatttgatt tcatattttt tactactctc ataccaaatt
    13201 ccatgtcctt aagttgtgca tctgttggtt tttcctttgt tacattttca cttttttctg
    13261 ctaatatatt gtttctatct tgtatcaata attttccatc taaatatttc atttcatatg
    13321 gttgtaaact attttctatt ttagctaatt ttaatattct tagatttttc ttttgtttaa
    13381 gtatctctaa agcttctggt tcaaaatcat atgctactac tatttctaag aatatttcat
    13441 ttaatttctc tgctgtagct ttatctatag tagaagttat tcctactatt cctccaaata
    13501 ttgaaacctt atccgcttca taacattttg tatatgcttc aaaagcatct tttcctaaac
    13561 ctaccccaca tgaattagca tgttttatag ctactgaaac tacttcttca ctatctttaa
    13621 attctctcat tacttctaaa catccatgta aatcatttaa gttgttaaaa gaaagttctt
    13681 taccgcctaa ttgctcataa tttaagattg gattttttgc atttgactgt gaatataaga
    13741 aaccattttg atgtggattt tctccatatc ttaaagtttg ttctttttgg aaagttaaat
    13801 ttaatatgtc aggatacgta tctccaactt ctccagcaaa ataactagat attaacgcat
    13861 catatcttcc tgtagttgag aaagctttat atgataattt ttttctgtct tctaatgtca
    13921 ttgtatctgt ttttaatttt tctattatag aatcataatc ttttatatct actactacta
    13981 ttacatcctt atagtttttg gcagcagaac gtatcataga tggtccacca atatctatat
    14041 tttcaattat ttcatcatga ctttttccag cttttaaagt tccttcaaaa tcatatagat
    14101 ttacagctac taaatctatt gaatgtattt tgtgttcatt tacagtttct acatgagatt
    14161 ctttatctct tttataaagt ataccaccat gtatatatgg atttaatgtt tttactcttc
    14221 catctaatat ttctggaaaa tttgtaacat cttcaacttg cattacatta acaccatttt
    14281 cttttaatgt tttaaatgta tttcctgtag atataatttc ataatctaat ttatttaact
    14341 ctttagcaaa ttccactaca ccagtcttgt cagttacact tattaaagct ctcttactca
    14401 tttataaaca ctctcctacc ttgaagtttt aatttatttt cacaaaaaag acttatactt
    14461 tcttttaaaa tcctatgttc aacttctaac actctttttg caagtgtctt tgcatcatca
    14521 tcttgattta ccttaactac atcttgcatt atgattggtc cagtatcagc accttcatca
    14581 acaaaatgaa ctgtagcacc agttacttta gctccgtaat ctataacacc ttgatgaact
    14641 ttttctccat agaatccagc tccacaaaat gaaggaatta atgatggatg tatgtttatc
    14701 attttgtttc taaactcatt aaccagcttt gggctgatta tttttaaata accagccaaa
    14761 actactaaat ctattttatt ttccttaaga atttctatta tcttgtcttc atcagtttca
    14821 caaattgctt ttatgttatg atttttagct ctctctatcc cataagcacc ttgcttactt
    14881 gagataacta ctttaacctg accttttatt tctccagact cagtaccatc tataactgct
    14941 tgtaaatttg ttcctccacc agatattaaa actcctatat ttagcataat tctacacctt
    15001 catgactatc tactacttca ccaattatat aagctttatc ttctaataat tcactatatt
    15061 ttttatctac ataagcagca ttgtcatttt ctctattatt tataaagttt acaacatcat
    15121 cagcattttc tttatctact attaaaacta gcccaatacc catgttaaaa ctcttatgaa
    15181 gttctctctc atcaacagca ttaaaacctt ctatcatttt aaatatagga ggcttctccc
    15241 aagatttttt attgatatct agtcctaacc ctttaggtat aactcttgtt atattttcta
    15301 taagccctcc accagttatg tgagctatag cttttatgtc atgtttagct aatacatcta
    15361 aagctaattt aacatatatt ttagttggtg ttaaaagtgc ttctccaact gtcattccta
    15421 gttcttcaac atattgttcc attttataat tgtaagtttc taaaaatatc tttcttataa
    15481 aagaaaatcc attgctgtgt actccacttg aagatatacc aaccaataca tctcctgatt
    15541 taacattgtt tccagaaaca atcttttctt tgtctgctat tcctacagaa aatcctgcta
    15601 aatcatagtc atcttctcca tacattccag gcatttcagc agtttctcca ccaattagtg
    15661 cacaaccaga cattttacaa ccatctgcaa ttccaccaac tattttttcg atatgctcag
    15721 gcactaattt cccaagagct atatagtcaa ggaagaataa tggttttgct ccttgacaaa
    15781 ttaaatcatt cacgcacatt gcaactaaat ctattccaac agtatcatgc ttgtccatca
    15841 tctgagctat ttttaactta gtcccaacac catctgtaga tgcaagcaaa actggttctt
    15901 tcatacctac aaaatctttt aaactgtata atccactgaa gttacctaaa tctccaacta
    15961 catttccatc ataagttcct tttattttac cttttataag gtctactgct ctatttcctt
    16021 catctatatc tacacctgat tctttatatg ttaacatgaa agtagttcct ccttatcaat
    16081 tttcttaact gggtattctc catcaaaaca agctttacag aaagtagact tatgttctgt
    16141 tgcatctaac attccttcta tatctaggaa ttttaaacta tcacatccaa tatattctct
    16201 catttcttct acgttgttac ttgatgcaat taatttagag cgatttggag tatctattcc
    16261 atagtaacaa gagtaagcta ctggaggaga agttattcta agatgaattt cttttgctcc
    16321 tgcttctctt aaagatttta ctaattgttt agatgtagtt cctcttacaa ttgagtcgtc
    16381 tactaaaatt atagattttc cttttattat tgttgaaaga ggatttaatt ttatttttac
    16441 tgctatttct ctttcttctt gcgtaggttt tataaatgtt cttcctacat atctattttt
    16501 aactaagcct tcacttattt ttaatccact agcatttgca taaccaatag ctcctggcca
    16561 acctgaatct ggaactggaa ctaccatatc tgcctttaca gtttcattct gagctaatct
    16621 ctcaccacat ttaattctaa attcatatgc atttacattg tctatagttg catcatttct
    16681 tgcaaagtat atgtgttcaa atatacaact tttcttaact ggtttataat tttctgaata
    16741 aaaatatgat tttagttctc catctttgac tactattatc tctcctggct ctacatctct
    16801 tataacttct ccgccaagta tatctattgc acaattttct gatgcaaata tatattcatc
    16861 acccttttta ccaagtacaa gaggtctgaa tccatgtggg tctctaactg caaccaattc
    16921 ttcttgactc attattacaa gagcatatgc accttttata taatccattg tcacttttaa
    16981 actttctact atatcccctt tgtaatatct agcaagtata tataaaatca cttctgtatc
    17041 tgaattagtt tggaacatat atccatcttc ttctagcata tcttttaaat aatttgcatt
    17101 aactagattt ccattatgag ctatggctaa ttgtcttttt ctacaacttc caactaaagg
    17161 ttgacagtta gaaacatgac ttcctccagc tgttgaatat ctaacatgtc caatccctat
    17221 atttcctttc aacttagata attcattttc cttaaaaaca tctccaacta atcccatatc
    17281 ttttttgtac ttaatttctc catcatctaa aagagctaaa ccacagcttt cttgccccct
    17341 gtgttgcata gaatataaag aataatatag ttctttagtt acatctttat tagagtaaat
    17401 ccctaaaact ccacacatac atttgcctcc tatttattat tcattcttga aagtacttcc
    17461 gtataacctt ctacaaggtc tccaaggtct tttctaaatc tgtccttatc taacttttca
    17521 tttgtattta catcccaaag tctacaagtg tctggtgata cttcatctgc caatattata
    17581 tttccttcag aatctttacc aaattctatt ttaaagtcaa ctaactttag gtttaatttt
    17641 aagaaaaatg cttttagtaa ctcgtttatt tttaaagttt cttctcttaa gaattttaac
    17701 tcttctctag ttgctaactt cattgccact gcataatcat catttagcat tgggtcacca
    17761 taagcatcat ttttgtagct tatttcaaaa attggctcat cgaaaactac accttcttca
    17821 agaccaactc ttttacagat agaaccagca gcaatatttc ttactattac ttctaatggt
    17881 aaaatctcta cttttttaac taacatttct ctatctgata aactttttat aaagtgagta
    17941 tttatattat tttcttttaa catttcaaag attattgttg atatcttatt atttagtata
    18001 ccttttgaag atatttctgc ttttttttct ccattaaatg ctgttgcatc atctttataa
    18061 taaacaacat attcattttc attatctgta gaatatactt gttttgcttt tccttcatat
    18121 aataacattt ctgctacccc cttaaattct tgtcgtcttc taacaccttt tgtgccatct
    18181 cttctctata tgatttcaaa tcctctttta attttgggta ttttaaagtc attatttgta
    18241 atgccaataa agctgcgttt aaacctaagt ctattgtaac tgttgcaact gggattcctt
    18301 ttggcatttg aactattgat aaaagtgagt ctagtccatc catagttgat gatttaatag
    18361 gtaatcctat tacaggaatt aatgtttgtg aagctattac acctggtaag tgtgctgctt
    18421 ttcctgctgc tgctattatt acatcagtat catcttctat ttcttttagg aatattgaaa
    18481 gttgttctgg tgttctatga gctgataatg ctcttgctac aacttctatt ccatattttt
    18541 caagcagttt tatcccttct tctaactttg gataatctga ttttgaaccc ataactactg
    18601 ctactttcat tagacttccc ctctccgttt ataagttatt ttacactaca aatcattcgt
    18661 attattatac gaattataca ccttaaattt tatattaata tacatatatt atcaactctt
    18721 ttttgaaatt ttttaaaaaa aatatttctt gtaatttttt cattcgctac gtatcgaact
    18781 ttatttatgt ttttttgtta aaataaaaaa cagattatct caaaattggt tttctaatta
    18841 aattttaagc ccaatactct tttttaaatc ttgtatatta ttttttatta cttctttttt
    18901 aattaatact ttgggtttat agtcttgttt tttagttctg aatgttactt ttacttagat
    18961 aaaaccaaca caaagtttat tctttatata agtaatagct aaaatttaaa gtaatgatat
    19021 tatataaata gaaataattt tatttaaagg agaatctgta tgttagaagt tataaaaaat
    19081 agacgttcaa taagaacata tatagataaa aatatagaag aagataaaat aactgaaata
    19141 ttaaaatcag ctatgcaagc accttcatca aaaaatgctc aaccttggga atttattata
    19201 gtagatgata aggaactgct gaaacagctc tcaaagtcac agcatagagc aaagcatata
    19261 gaatttgcac cattatgtat agtagtgctt ggaaatagag ataaattctt aaaacctggt
    19321 aaatggatac aagatttagg tgcttgcaca caaaatttat tattagaagt tactaatcaa
    19381 ggtctagctg cttgttgggc aggagtattt cctaaaaata aagtggtaaa taaagttaga
    19441 caaactttag acttaccatt aaaattagtt ccttatgcac ttatatctat tggatatagt
    19501 gaagaaaaaa atgagtttat agaccgattt gatgaaaata aaatacatag aaatgtatat
    19561 aaaaatagat aatgtttagt aatataaatg aaaatacata gtaaaaatgc ctttagaata
    19621 catttgtatt ctaaaggcat taactttgac taaacaccta attagcctct aaggtaaaat
    19681 ttaactatca tttattattt atataccaat tgaatattat atccttcaat atttttattt
    19741 atttcaaata tattaagaat atcctacaat taaagttagc tatttttcat taaagtattt
    19801 tattgacaca ctccccatag ctaaagcaag gggattcttg gtggctaaca cacttattta
    19861 tacttaaaca gtacaacaga ggtggttaca aagccaagct acttcggacg gtgtcttcgc
    19921 cactactacc ttaaagttat tcgcccacac atggctcaaa cggtagatac ttttaaattt
    19981 attggttgct tatgatttat tctaaaatct actaaaccat atttatgctt tttatttaaa
    20041 atattccaag caccaactat atctctatga gtttcataac cacaagtaca actataagtt
    20101 ctaccagttg gtttgtgttt tttagaacac tttgggcaag tttgagaagt gtagttttct
    20161 ttaactaact taacttcaat accttgctct tttgctttat attttatata gtcctttatt
    20221 cgaccatact ccatttgaga tattttctgt ctattaactc tgtttaatct tttctttttc
    20281 ttagtatctt tttcaacccc ttttatatcc ccaagaacta tttcacatac cttctgctct
    20341 acacaaaagt ctataaactt tctagttgtt tgatgatata aattcattaa ctgattatct
    20401 gttttacttc ttatcttatt ttttaatctc cataacttct ttgattgtct actacctttt
    20461 ttacatttac ttaatctttt agatatataa gcatacatct tatttctaaa ttgtttaata
    20521 cttctgcctt ctctattaga tagaataagc cctacacctt tatttgttac actagctatc
    20581 gaatgtattt cccctaagtc tacacctacg gtattagtgc ctttggctac aactccattt
    20641 aattcattgt caaaagtgta gtataaataa tatccatttc gccatactat ttcagcagtt
    20701 tttatattct caacattaaa atctaattct aaatacctgc cttttgacat tgataatttt
    20761 aagttgcctt tagcatttct attaatagca ctagctttaa aaggtattat ataaaatttc
    20821 ttagtcttgt atggatattt tgctttagta ttaccttttt ttcttaattc tgatatagtc
    20881 tttctatttg catcaaattt atctgatata gcttgaatag tttgactatg aagattgtac
    20941 ttacctttga ttaacttttg tatatcattc tttttaatcc attgtttatg ttcaaaatag
    21001 tattctttag ataagttaac tatgtagttc caacagttag cactttccat ttgcttttcc
    21061 cttactaatt caaattgtgt tctagttagg tttaatttca ctctatgagt ttttatcatt
    21121 tctttgtcct aacttgttga ttttctatat attgtttaac tacttctaaa ctcacagaac
    21181 caacagtacc tacaaaatag ctattagtcc acatgctagg catactagac tttaaatgtg
    21241 gaaagttctc tcttaatact ctcgaactaa agccttttaa accttttact actttatgaa
    21301 ttccatattg agggtcacaa gatattaata agtgcacatg ttctttatca gtttccattt
    21361 ctattatttc tgctccaagt tcatctgctt tatatggtag tatttctttt aacatctttt
    21421 caacttcgcc aactaaaaca ggtttacgat acttagggca aaatacaata tgatatttgc
    21481 aatcataaac tatatttttg ttagatttat acatgaaaac taacacctcc taaatatagg
    21541 gtatcagtag cggtattaga ttacaagcat tattctaact ttttttatat taagacctta
    21601 cctttcatcc ctacacctaa aggagagggc ttttcggcaa ttttattgta aattaataat
    21661 gataatcaca actttaagaa aaatataacc cttaatgatt atcataaaaa ttaatttttt
    21721 gaaaaaactc atattgactc ttgaagaagt acatacaaat aaattttgct ttatgaatat
    21781 tcaaataatt tattttatag atatttaatg ataaatatcc tacaaaatta tattaatata
    21841 ttaggtcgac taaattttga tataaagaga atataaaata ctgtaaaaaa gtgtaatggt
    21901 tatttccttg aaaagaaaga catactatta aagcataaat tacaatatag gagatgatta
    21961 aatgagaata ctttcagaaa ctgaaaaaat atctttagca gcaattataa aaatggagtc
    22021 tgacggatta cttatgcaaa gagcaataaa tatgttaata tcagatgaag acctaaaaag
    22081 acaatcagaa tctagtatat tagcaacaga aggaagaatt aaagctatac aacaatttat
    22141 agttgaaaat gaaatactaa tttctgagga ggtatagata acatgagaaa tatgatagaa
    22201 aatctaatta agaacaatac ttatatagac gataaagtaa taatgttaag tatgctttca
    22261 tctgctaaag catctgccaa tatgtatctt aattctgcat tgacaagtag tacacctgaa
    22321 ttaagagcta tatattcagc ttcattaact caaatggtag aaggtcacac agcacttaca
    22381 gaattaagta ttaataaagg atgggttaaa ccatatgata aacctattca acagttaaca
    22441 tgtagttata aagaatccca aaatgttatt gattaa
//
LOCUS       DACXMZ010000045        21195 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.045, whole genome shotgun sequence.
ACCESSION   DACXMZ010000045 DACXMZ010000000
VERSION     DACXMZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.045"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            72..3020
                     /locus_tag="JD016_06695"
     CDS             72..3020
                     /locus_tag="JD016_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU4999324.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEVISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            2998..3462
                     /locus_tag="JD016_06700"
     CDS             2998..3462
                     /locus_tag="JD016_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAU4999325.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3575..3877
                     /locus_tag="JD016_06705"
     CDS             3575..3877
                     /locus_tag="JD016_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU4999326.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            3954..5303
                     /locus_tag="JD016_06710"
     CDS             3954..5303
                     /locus_tag="JD016_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086506.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAU4999327.1"
                     /translation="MEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGLTVGVG
                     FIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALIIPIGL
                     VVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIVLVMAD
                     LTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQKKFGL
                     FGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLPVSEAA
                     QEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVLPFADL
                     AVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFKMPEGA
                     TMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKLSIEE"
     gene            5338..5811
                     /locus_tag="JD016_06715"
     CDS             5338..5811
                     /locus_tag="JD016_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999328.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YVKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            5983..6633
                     /locus_tag="JD016_06720"
     CDS             5983..6633
                     /locus_tag="JD016_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999329.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6807..7739
                     /locus_tag="JD016_06725"
     CDS             6807..7739
                     /locus_tag="JD016_06725"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAU4999330.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYYDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKNRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            8013..8726
                     /gene="ispD"
                     /locus_tag="JD016_06730"
     CDS             8013..8726
                     /gene="ispD"
                     /locus_tag="JD016_06730"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAU4999331.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8723..9208
                     /locus_tag="JD016_06735"
     CDS             8723..9208
                     /locus_tag="JD016_06735"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAU4999332.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9303..9511
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9575..11290
                     /locus_tag="JD016_06740"
     CDS             9575..11290
                     /locus_tag="JD016_06740"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAU4999333.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11412..12857
                     /locus_tag="JD016_06745"
     CDS             11412..12857
                     /locus_tag="JD016_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Proline--tRNA ligase 2"
                     /protein_id="HAU4999334.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13295..14776
                     /locus_tag="JD016_06750"
     CDS             13295..14776
                     /locus_tag="JD016_06750"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAU4999335.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            14997..15152
                     /locus_tag="JD016_06755"
     CDS             14997..15152
                     /locus_tag="JD016_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999336.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15297..16694
                     /locus_tag="JD016_06760"
     CDS             15297..16694
                     /locus_tag="JD016_06760"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAU4999337.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFVK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            16697..17092
                     /locus_tag="JD016_06765"
     CDS             16697..17092
                     /locus_tag="JD016_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HAU4999338.1"
                     /translation="MEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRKVNDLHK
                     LAIKYVKAKAQATIIHEVEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKHATGFEA
                     LVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17198..17956
                     /locus_tag="JD016_06770"
     CDS             17198..17956
                     /locus_tag="JD016_06770"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HAU4999339.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            17960..18706
                     /gene="rlmB"
                     /locus_tag="JD016_06775"
     CDS             17960..18706
                     /gene="rlmB"
                     /locus_tag="JD016_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAU4999340.1"
                     /translation="MNNLASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMA
                     KDKNIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIIL
                     DEITDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIV
                     NTIKRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMP
                     MIGNVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            18709..19236
                     /locus_tag="JD016_06780"
     CDS             18709..19236
                     /locus_tag="JD016_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAU4999341.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            19286..19948
                     /gene="sigH"
                     /locus_tag="JD016_06785"
     CDS             19286..19948
                     /gene="sigH"
                     /locus_tag="JD016_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HAU4999342.1"
                     /translation="MGEIYMLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIIT
                     KYRNFVKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQI
                     ITAIKTATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKN
                     IESKMNELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENR
                     ND"
     gene            20022..>21195
                     /gene="tuf"
                     /locus_tag="JD016_06790"
     CDS             20022..>21195
                     /gene="tuf"
                     /locus_tag="JD016_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAU4999343.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVE
                     DVFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLDQAQAGD
                     NIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFDGYRPQF
                     YFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRTVASGV"
BASE COUNT         8763 a         2225 c         4018 g         6189 t
ORIGIN      
        1 taataagtac aaaaaaaaca atatatggca tataagttgc tatataaata ataaggaggg
       61 aacaattata aatgaggcaa agaataaaaa acttaattga aaatgaagat aagaaaaatc
      121 ctttaacaga tgaagtgatt tcaagtaggt taaatattcg aagagaagat gttacctttt
      181 tgagaaatga gttaaaaata gaagattcaa gacaaagaag aaaagttgtt ttaattaaag
      241 ctataaaaga tattttaaaa gaagaaaaaa ctataaataa aaatgatata acaagaagat
      301 taaacagcat tgggtttaca gtatcaagat ttacagtcat acaatactta aaagagattc
      361 aagattctgg tgacctaaaa cttgataaag agagtgatga taaaaaagaa gaaataatca
      421 ataatagaga aaataaagat atagcatttg ataaactcat aggtagtagt ggtagcttaa
      481 gtacaattat aaaactagcc aaagcagcaa ttctttatcc acctcatggt cttcatacta
      541 ttattatggg gccaactggt gttggaaaga gtgagttagc agagtgtatg tataaatttg
      601 cagtagaatc atccagattc cctaagaatg caccatttat agtttttaat gcagcagatt
      661 attcagagaa tccacagctt ttgctatcac agttatttgg acacgttaaa ggtgctttta
      721 caggagctga tgagaacaaa gaaggattag tatcaaaggc agatggagga atattgttta
      781 tagatgaaat acatagatta cctcatgaag gtcaagaaat actatttcaa ctgattgata
      841 aaggtatggt cagaaagtta ggagaaacaa gactaacaca taaaatagat gtaatgatta
      901 tttcagcaac ttctgagcca atagattctc atttacttaa tacatttaaa agacgtatac
      961 ctgtaacaat tgaaatacca gaattagttg caagaccatt aaatgagagg tttgacatta
     1021 taaataattt cttcttaata gaagctcaaa gaatgggagc aaacatacat ataaagtcag
     1081 atgtattaaa agctcttatg ctttacgatt gtataggtaa tgtaggtcaa cttagaagtg
     1141 atattcaagt agcttgtgct aggggactat taaaccaact tacaaacaaa ctaaaagaag
     1201 taaatataag catatcagat gtacctggat atgtaaaaca aggtcttata aaaatcagaa
     1261 attgtagagg caaaatagaa aattatgtag atggggattt aattatagat tcaaatcttc
     1321 aaggtgagat agaaaagaaa aatgaagata tatatacttt tccagatgaa atatacaaac
     1381 ttactgaaag aagacatatt gatttattgg aacaaggttt agattttgat gttataaata
     1441 gaatcattgg aggagaatta gaaagttgca ttcaaaaata cataaaacaa gttaaaaaag
     1501 tttctattag taacaatctt ccagatataa gtccaattgt tggaaaatat atcgtagagc
     1561 taacttatga agtaattaaa atagcatcac aatacctcag ttcactagaa ccgaatctac
     1621 atttatgttt agcagttcac ctaaaagcag cttttgagag attaaaagaa ggaaaagtaa
     1681 tatcaaatgc tcatttgaaa gaaataatag gaaattatcc agtagaatat tctgtagcag
     1741 aaaaggtagc tcaatattta aaggaaagat ataacataaa cttaccaaaa gaagaaatag
     1801 gtataatagc aatatatttt aaaatgagta gtagaagaga tatgtctgaa caaaaaaaga
     1861 taggcgtact tcttatggct cacggaagag tagcatcttc tgtatgtgag gttacaaata
     1921 aactattagg aattaatcac gcaaggtatt tagatatgcc atttgagaag aagccggaag
     1981 aaatgattga agaagctact acaataataa aagatattga tgaaggtaga ggtgtgatta
     2041 tattagtaga tatgggttca ctttcatatt ttggagatat tatttctgga aagactggaa
     2101 ttaatataag gacaatatct agatttgata cattgctttc tatagaggtt atacgaagag
     2161 cagttttacc agatttaaca atagacgaga tagttgatga agttaaaggt atagagccac
     2221 aagtaaaaga aaaggttagt aaatgtaaca atacaaaagt agattttaga aaacctataa
     2281 ttttaacatt atgtattact ggattagggg gagcttgtaa gataaaaaaa atcttagagg
     2341 aaaatattaa aagtataact gaaaaggttg atatagaacc tataggtatg attgaatatc
     2401 caagtcttga tttagagata aaacaaataa gcaatgataa aaatgtaatc gccatagttg
     2461 gaacaattga ccctcagata gcaaatatcc ctttttttag tctagtagac ataaatgata
     2521 aagcaaagtt aaatcaaata aataacctaa ttgatacaaa tcaaattggt gaaacaaata
     2581 aaaaatatga attatcagat ttaatacata aagaagatat atttgtggat ttacaggtta
     2641 aatcaaaaga agaagttcta aaatatatga gtaaaaaact tagagagaaa ggttatgtga
     2701 caaaaaattt ctataaaaaa gtagtggaaa gagataactt atttccaact gaattatcag
     2761 atgaaatagc aatacctcac acagatggaa tagatgtaat taggccagct ataagtatag
     2821 ccatcttaaa gaagcctatt gtgtggagta aaagtaaagt aaaaataata cttttattag
     2881 ctgtagacca aaagtgtttt aatccattaa gcactctact ctcatttata gaaacagatg
     2941 aatttaagaa gataaaaaac attaaagata gagattttat tagggagatg atattagatg
     3001 gagtcaatga aaataattag tagcaattta atttttaaaa atattgaagt gtctaacaat
     3061 gaagatgcat taaagttttt gggacaacgt ttgtttgacg aacagtatgt aaaagaaagc
     3121 tacatacaag ctgttgtagc aagagaaaaa aagtatgcta cagggttacc aacagaaata
     3181 tacggagtgg caataccaca tacagatata gtacatgtaa atgagccagg aatagcaatt
     3241 ggaatactga ataaacctgt aaaatttata atgatgggta cagacgatac tgaaattgat
     3301 gtaaaagttg tatttatgtt agcagtgaaa gagccacaag aacaattgca actattagaa
     3361 aaacttatga ctatatttca agataagaat atactaaata atattgttga cttaagtgaa
     3421 gaaagtgtga gtgatttatt aaactctaaa ttgaaaaatt aaacatgtaa agtatattaa
     3481 aaatgtaaaa ttaaacatgc aaagtatgtt aaaaaatata aaaagattat taaagatttc
     3541 taactataga tatctttaaa ataaaataat ttcaatgggg gtgactaatg tggctaaaaa
     3601 gatattagtt gcatgtggaa caggagtatg tacttcaaca attgctataa acaaattaaa
     3661 gaaagcactt caagacattg gtaaattgga tatggttaat attacacaat gtaaagtagt
     3721 agaagttgca tcaaaagcac ctgattatga tttaataatt tgtactacac aagtatctag
     3781 tagtataaaa acaccagtga taaatggctt acctttttta actggtgtcg gaatggataa
     3841 attaatcaat gatgtattag aagagttaga attataatta aaaaaattaa ggaacatggt
     3901 ctctaaaatc ataagtttta gagacttaaa attaaataat ggagggtctt tatatggaga
     3961 tattaaattt cattgttggt ctaggtgcac aagtaatgat gccaataata atatgtattt
     4021 ttggtttgat attaggtaca aaactaggaa aatctttaag agctggttta actgtgggag
     4081 ttgggtttat aggtcttaac actattatag ctttgctaac agataattta ggtccagcta
     4141 cacaacaaat ggttaagaat ttagggctaa gtttatcaat aatagatgta ggatggccag
     4201 cagcatcagc tatagctttt gcatcaacag taggggcttt aattattcca ataggtcttg
     4261 tggtaaatat agttatgcta attactaata ctactcaaac tgttgatgta gacatatggg
     4321 attactggca ttttgctttt acaggagctt tagtggcagg ggctactcaa agtgtaatgt
     4381 ggggagtatt tgcagcagta gcaaatatgg taattgtatt ggtaatggca gatttaactg
     4441 ctccaggtat agaggaatat ttaggtatgc caggtatatc tcttcctcat ggatttacac
     4501 aggcatttgt tccaattgct atagtagtaa ataaattatt agacctaata ccaggcataa
     4561 ataagattga gataaatgct gatacattgc agaaaaaatt tggattattt ggagaacctt
     4621 taataatggg gtctgtaata ggtgtaataa ttggtattgc tgcgaaatat gatataaaag
     4681 gaatattaca attaggtgta actatgggag ctgtattaat attaattcca aagatggcag
     4741 cattattaat ggaaggatta ttaccagtat cagaagctgc acaggaattt attgagaaga
     4801 gatttaaaaa cagaggcaag atttatatag gcttagattc agcagtagga ataggtcatc
     4861 ctgttacatt atcagttgca ctagtgttgg ttcctttgac tatacttata gcagctatat
     4921 tgccaggaaa taaagtttta ccatttgcag atttagcagt aattccattt gcacttgtat
     4981 taatcgtacc aattacaaag ggaaatgtgt ttagaacatt aataatagga ataattataa
     5041 ttacttctgg attattaatc gcaactaact tagctccatt atttacccaa atggctttaa
     5101 atgctagttt taaaatgcct gaaggagcaa ctatgatttc aagtatttgt gatggtgcga
     5161 atccattaag ttgggtattt gtaaaagtta tgaactataa agttattgga ggagtagttt
     5221 ttggagttat agctttagga atggctattt acaataggaa tagaataata aatgaaaata
     5281 aaaagctatc tatagaagaa taaggctaaa acaattttat taaggagtgt ataggttatg
     5341 aaaaaatata aatatgattc taaaagatac ttactcagtg ttacaatacc aggatttttt
     5401 atgattttaa tattgattta tgcactgttt aataactata caaattttaa tttgaatata
     5461 tatagtttgt taataattgt ctgtacttat ggtatattca atacttttat atcatcaagt
     5521 aatcctaaaa aaattattat tgatagcaaa tgtatacatt ttacctcatt tatgactaca
     5581 cataagtatg aaattaaaga tatagaaaag ataagaataa gagaatttta taataaaaaa
     5641 atttatgtaa aaattaatga tggaaacctt tttaaaggta ggtattggat aagaacaaat
     5701 atgtttaata atagtatagc attatatagc tatttcattg agttggaaga gtgcttatat
     5761 ccagattcac taaaatttag aaataaaaaa tttgagaata agaacatata aaaataaaaa
     5821 attgaattga agcctagctt aatctttttt gataagatat aagtttgaac tttatactaa
     5881 atttttagaa ttttcaatca agacaataac ataatagcta agcaattaga acacaattaa
     5941 gatgtataag gcatgtatat gatgaatggg aggatttaat taatgttatt agagaaatta
     6001 agaaaagaag tattacaagc atctttagac cttttaaact ataatttagt gacattaaca
     6061 ggaggaaatg taagtgggag agatgaacaa actggctata tagcaatcac cccaagtgga
     6121 atggattata gaaatcttac accaagtgat attgttatag tagatgtaga tggaaatatt
     6181 atagatggaa aatggaaagc atcagtggat ttatcagacc acttgtatat atataagcat
     6241 agagaagaca taaatagtat aattcataca cattctacat actcaagttg ttttgctatt
     6301 ttaaatgaac ctatagaatg tgcaagtaca actcttgcaa atgaagtagg gggttcagta
     6361 ccagttgcaa aattttcacc accaacatct aaaaagatgg gaaaatgtgt tattgaagca
     6421 attggtgaca aaagagcatg tcttttagca aatcatggcg taattgcagt tggtccatct
     6481 gtaggacatg ctcttacagc cgctgtaatg ttagaagatt cagcaaaagt atattactta
     6541 gcaaaatcaa ttggcacacc agtgctactg cctgatgaag aaattcaaag agcgagagat
     6601 gtatttttta atgtctatgg tcaggataaa taaattagat aaaattttaa aagtaatatg
     6661 aaaatataga tattcaatga tattaaggag atttataatg atataataag tttaatagtt
     6721 aagaagtaaa atatgaaatg taatagtata taaattcatt taagactaaa aagaataaga
     6781 taaaataaaa aggagtctgt atttctatgt attacttatt aaacaaaagc acaaatcctt
     6841 actttaattt ggcattagaa gaatatctat ttttaaatga taaatataat gatgatatta
     6901 taatcatatg gagaaatgaa gaatccattt ttataggtaa aaatcaaaat ccatatcagg
     6961 aagtatatta tgatgttatt gaaaagggag aaattcctat tttaagaaga atatctggag
     7021 gaggaactgt ttatcatgac ttaggtaata ttaatatgtc attcatacaa aaaaataggc
     7081 aacttcatga gatagatttt ttggagcaca ctaagtttat gcaaaagatg ctttcaacct
     7141 taggactaga tgtatctata actgaaagaa aagatttatt tttaaatggg aaaaaaattt
     7201 caggttctgc acagtctata aaaagaaaaa attctcttta tcatggaact cttctttacg
     7261 attcagattt aaataaatta actaaatatt taaattcaaa taaagcaact gaatcaaatg
     7321 caactaaatc tgtttcaagt aaagttacaa atattaagtt tttactcgaa aaagatataa
     7381 attacttttt agattattgt gttgaatatt taaaatctaa tataaagaat ataaaagaac
     7441 ttgaattgga tgaagaagat attaaaagtg tatacaatat aggagaagat aaatataaaa
     7501 agatagagtg gacatatggt aaaacgccga aatttcaagt agtgctacca tttaataatg
     7561 aaagtaaaat aaatatacat gttaacagat ggaaagtatc aaaatttttt atttcttatg
     7621 atgataatgt aattaatatg gacaagttgt tagatttgaa tttctttaaa gaagaaataa
     7681 aagagagtat tttagaaaat tatccacatt acatagattt aatcaattta atattttaat
     7741 ataaaacctc taatttgata aaattgtctt atatcaaact agaggttttt aatattcttg
     7801 catattaaca accattacaa aattgtaaaa ttaaaaacaa cactattgaa aaatcaatat
     7861 atattgtaat aaatatatgt gtttttcatt agcaaataga cattaactat ttatacaact
     7921 gaaaatttaa tatataatta atcagatggt taaaataaaa atattaaaag ttttaataaa
     7981 tgatggttaa aaataaaata cgaggtgata ccatgtatag tgttataatt gttgcagcgg
     8041 gaagcggaag gagaatgaac ttagatataa acaaacagtt cataaaatta agagaaaaag
     8101 aaattattgc tcatacaatt caagtttttt atgaaaatat aaatatagat gaaatagtgg
     8161 tatgtataaa aaaagaagaa gaagattttt ttaaagaaaa tataataaat aagtataact
     8221 ttaaaaatat aaaaatagca tatggcggaa aagaaagaca ggactcaata tataatggtt
     8281 taaaaaaatt agatgaaaat tgtgatatag tacttatcca tgatggagca agaccatttg
     8341 tcgaccatag aataatcaat gaatctataa aggtagctaa agaaaaaaag gctgtggttg
     8401 taggtgttcc agtaagtgat actataaaga tagtgtcaga tggaacagta caagaaactc
     8461 cagaaagaaa tttgctgtgg gcagcacaaa cccctcaaac ctttgaatat aatctcataa
     8521 tagatgctta tgaacaagca tataagaaca attattatgg tactgatgat tcaatgttgg
     8581 tagaaaatat aggtcaaagt gtcactatgg taatgggttc ttatgaaaac ataaaaataa
     8641 ctagtccaga agatttaaat atagcagaac aaatattaaa tatggaaaaa agagatgaag
     8701 ttaacagtag aaggagaatt atatgagaat aggattagga tatgatgtac acaaattaac
     8761 agaggataga aaacttataa tcggtggtgt agagatacca cacgataaag gtttgttggg
     8821 tcattctgat gctgatgtat tgatacatgc aataatggat tctattttag gtgctctagc
     8881 attaggagat atagggaagc atttcccaga tacagatgaa gaatataagg gagcagatag
     8941 tatgaaatta ttagaacatg tgtataatct aataacaagt aaaggatata aaattggtaa
     9001 tatagacagt actataattg ctcaaagccc taagatggct ccatatattg agagtatgag
     9061 aagtaatata tcaaaagttt taaacacaga cattgataat ataaacataa aggcaacaac
     9121 agaagaaggt cttggtttta caggagcaaa acagggaata gcttcacaaa gtatttgttt
     9181 attattgttg acaagtcaga ataattaata tattattagt aataagttta atggatatca
     9241 agtaataggc ctatatagaa tatctataat gtatgaattt aagtgaaatt aatataaata
     9301 taaaattagc tatgataaag aaatagtaaa atgatgaaat cttcacagag agaaaacggt
     9361 ggtgagagtt ttcaagagga agtctttgaa cctgtctttt agcttaaagg tataaaaatc
     9421 tttacggtag gaaccgttat attcccaaaa gagagctatt catatagcta actagagtgg
     9481 taccgcggaa attaaacctt tcgtctctat acttgtatag agatgagagg ttttttatat
     9541 tttcaggaag ttaatattca ggaggaatct attaatgaaa atgtcaaaaa tgtttatgcc
     9601 gacattaaaa gaaatcccag cagatgctga gattacaagc catcaattaa tggttaggtc
     9661 aggcatgata aaaaaaatga cttcaggtgt ttataaccaa ttaccaatgg ggttaagagt
     9721 gttcaaaaaa attgaacaaa taattagaga agaattaaat aaaaaagatt gtcaagaaat
     9781 actttgtgca gccttacttc cttctgaatt atggaaagag tctggaaggt ggactgctat
     9841 gggtgaagaa atgtttagac taaaagacag aacagaaaga gagtactgtc ttgggccaac
     9901 acatgaagaa gcattcactg atataataag acaagaaatt acttcatata aacaattgcc
     9961 actaaacttg taccaaatac aagtaaaata tagagacgag agaagaccaa gatttggtgt
    10021 tatgagaact aagactttta caatgaagga tgcctatagt tttgatgtag atgataaagg
    10081 tttagataag tcatatcaag atatgtttga tgcatatgta agtatatttg atagatgtgg
    10141 attggagaat agccctgtac aggcagattc aggagctata ggaggttcta catcagccga
    10201 atttatggtt aagtcagaag ttggtgaaga tgaagttgta ttttgtagtg gatgtgatta
    10261 tgcagccaat gtagaaagag cagaatcttg taatctagca tcacaaaaag aagaaatgaa
    10321 agaattagaa gaggttcata caccaggagc agctacaata aaagagttag aagaattttt
    10381 aaaaacttct ccagataagt ttgctaaaac tttagtatat gaggcagatg gaaaaacagt
    10441 tgtagtcgtt gtaagaggag atagagaagt taatgaaatc aaagtatcta atgccatagg
    10501 ttctgttata gaatttgctc ttgctacaga tgatgtagtg agaaaagtaa ctaatgcaga
    10561 agtaggattt gcaggtccaa taggtataaa tgctgactat gtatttatag ataaagaaat
    10621 tgttgaacaa agaaacatag ttgttggggc taataaaact gagtaccata taaaaaatgc
    10681 taactatgga agagattttg aaggaatagt tggagatttt agaaatgttc aagaaggcga
    10741 caagtgtata gtatgtggta agccacttga aatagctaga ggtgttgaag taggacatat
    10801 atttaaacta ggaactaagt actcagaatc tatgaatgca aacttcatag acaaagatgg
    10861 aaaatcaaag cctatagtaa tgggatgtta tggaataggt gttgaaagaa cagcagcagc
    10921 tataattgaa caacataatg atgagaaagg tataatatgg cctttgtcag tagctccata
    10981 ccatgttgtg atagttccag caaatatgaa aaatgaagaa caaatatcaa tagctgaaaa
    11041 tatatataat gatttgcaag ctatgggtgt agaagtttta ttagatgaca gagatgaaag
    11101 aataggtgtc aaattcaatg attcagaatt aataggaata cctatgagga ttacagtagg
    11161 taaaaatatt aatgaaggta aagtagaatt taaacttaga cataaagaag ataaagagat
    11221 aattgacata gaagaaataa atgaaaaagt aaaggcagag tttatcagga ataatgttag
    11281 gttaggataa taacttaaat aagatattgt atagataatc aaagttgtac tttatagaca
    11341 ccattttatc tatatataag gatataaaat ggtgtttttt tataaaacaa ataaatgagg
    11401 aggtaattga aatggcaaaa aatgaaaaac aatttgttga agaaataaca aagatggaag
    11461 atgattttcc acaatggtat acagatgtaa taactaagac tgacttagtt gattatgcac
    11521 cagtaaaagg ttttatggtt gtaaagccat atggatatgc attatgggaa aagatgcaag
    11581 aatttatgga taaaaaattt aaagaaactg gacataaaaa ctgttatttt ccattattaa
    11641 taccagagag tttattaaac aaagaagcag aacacgttga aggttttgct ccagaagttg
    11701 catgggtaac acatggagga aataaaaaat tagaagaaag attatgtgta agacctactt
    11761 cagagactat aatatgtaca atgtatgcga aatggttaaa atcatataga gaattaccat
    11821 atctttataa tcaatggtgc tcagttgtta gatgggaaaa atcaactaga cctttcttaa
    11881 gaacatctga gtttttatgg caagaaggtc atacgttaca tgaaacagca gaagaagcac
    11941 aagaagaaac aatacaacaa ttagaagtat ataaagcgct atgtgaagaa ttacttgcaa
    12001 tgccagttgt agctggtcaa aaaagtgaaa gtgagaaatt tgcaggtggt gaaagaacat
    12061 atactataga agctatgatg catgatggta aagcattaca atcaggtaca agtcacttcc
    12121 taggtcaaca ctttactaag gcttttgata ttacttttgc agatagggaa ggaaatttag
    12181 ctaatccata ccatacatct tggggagcat ctacaagact tataggtgga cttataatga
    12241 ctcatagtga taacagaggt ttagtattac caccaagagt agctccaatt caagttgtta
    12301 tagttcctat agctgctaaa aaaggaaatg taatggagac tgttgataaa atatatgctg
    12361 atttaaaagc taagggagta gctgtagaag ttgatgatag agataactat acaactggat
    12421 ggaaatttaa tgagtgggaa atgaaaggtg ttccagtaag agttgaaatt ggaccaaaag
    12481 atatagaaaa taatcaagct atggtattta gaagagatac tttagaaaag gattctatgc
    12541 ctttagaagg attagctgat gctatatgtg atttatttga tgttatacac aatgatatgt
    12601 ttgaaaaagc aagaaaacat agagaagaca atacatctat agttgaaaat atggatgagt
    12661 ttagaaaagc tttagaagaa aagccaggat tcataaagac tatgtggtgt ggagatgcag
    12721 aatgtgaagc taagattaag gaagaaacag gagcaactat aagatgctta ccttttgaac
    12781 aagaaaactt aggtcacaag tgtgtgtact gtggaaaaga agcagatagt atggtagtaa
    12841 tggcaaaagc ttactaaaat acatttaaat aaaaactggg gtagaaagta tttaatactt
    12901 tatcccagtt ttttgttttt tatgagtaat aaaatgtatt tgtaactatg tcataagata
    12961 agtcatagat acttatatta aaaagaatat aatagagaac tatttttaat aattacatat
    13021 taatgaaagc aaaatatttt gttatataga ttttcaataa agcaaacaat aaatataaat
    13081 gaatattaat aaggagtgct tttaattttt agaaaataaa tgacttaaaa gaaagattta
    13141 tgaaattaaa actaggattt tctgatataa taaacatgtg tatttttaaa taaaaaggat
    13201 attattatta gatttgatta aaaataaact tgcaaaacaa tgtactagtt taatattata
    13261 gttatattga ataaaatatc aggaggaagt aaaaatgaat gtaagagtaa ggtttgcacc
    13321 aagtccaaca ggatttgtac atataggtag tttgagaact gctttatata actatttatt
    13381 tgcaaaaaaa atgggcggag aatatatatt aagagtggaa gatacagacc aaagtagatt
    13441 agtagaaggt gctatagaga atatgcttaa tgctatgaaa tgggctgggg tcaaccatag
    13501 tgaaggtgtt atattagatg atagtggaaa agtagtacaa aaaggagaat atggtccata
    13561 tattcaatct caaagattag atatatacca agaatatata aagcaacttt tagatagtgg
    13621 aaaagcttat tactgtttct gtacaaaaga aagactggat gaagtaagag atgctcaaag
    13681 agcagcagga gaaacagcta aatatgatgg tcattgtaag aatctttcta aagaagaagt
    13741 agaagcgaat ataaaagcag gaataccata tgttataaga ttaagattgc cagagaatca
    13801 tacaattaag tttacagact tagttagagg agatatggaa tttaatacta atgacttaga
    13861 tgaccaagta ttaatgaaaa cagatggatt tccaacttat cattttgcag tagttgttga
    13921 tgactatttg atgaaaatta cacatgttat aagaggagaa gaatgggtat catctactcc
    13981 aaaacatgtt tatttatatg aagcttttgg atgggaagca cctgtatttg tgcatttacc
    14041 aaatatactt aataaagaaa agaaaaaatt aagtaaaaga caaggtgatg tagctgttga
    14101 agactttaag aaaaaaggat atttaccaga gggattagta aactatgtag cattagttgg
    14161 ttggtctcct gaagataatc aagaattgtt tacaatggag gaattagaaa aagccttctc
    14221 tgtagaaagg gtatctaaaa gtggtggagt atttgatact gaaaaattaa attgggttaa
    14281 tcagcactat ataaaagatg gtgatgatgc ttatctaaca gacttagcaa taccattttt
    14341 aatagaagat ggatttataa cagaagaaga agctactaat aaatatgatt tcttaaagag
    14401 tatgatatca gtacttaaag agaaattaca atatgtaaaa gaagttactg aacatgcaag
    14461 tatatttttt ggagataaaa tagaggtaga aactgaagag ggaagagagt tcttaagatt
    14521 agaacatata cctactttaa tagatgcttt gagagaaaaa atagaaaaaa cagaggtatt
    14581 aaatgctgag tttgttcaag ctatgcttaa agaaatacaa aaagagtaca aaataaaagg
    14641 gaaaaattta tttatgggct caagaataat tttgacaggg caaatgcatg gtccagattt
    14701 accaaaagta atggaagttt taggaaaaga aacttgctta aatagaatag cttatgtaaa
    14761 aaataatata ttatagtcat aaaagtccgc taatttagaa gcggactttt tttgtgttag
    14821 tatttggtga aaatatctgc ttaagaaaat gaaggtattt aaggtaataa atttaatata
    14881 taaaaagcaa agcaaaaata taaataaaat atgaataata aactaataaa atattaagca
    14941 tagaagaagg gaatctatta ttttagtttg atttaattat taagggggta attactatgt
    15001 atagtaatat tgatgatgtt aaaaaggaat taaaggagct atgcttagaa tatgttacaa
    15061 ttttagaaaa attaaaggat gaaaaaatga ttacagaaga aacttttgaa aagtgctctt
    15121 ctcaaaaaaa aatattttta gaagaacaat agaggatggt aatagtatta ttattatgta
    15181 gtttgaaaga tagtacgaaa aatgatagta atactattat ttattgcaat tttcaactat
    15241 aatataatat ataatgttaa gaagataatt tataaaaata cagtgaggtg atgagtgtga
    15301 aagtttataa tacattgact agaacaaaag aagaatttgt tccattagaa gaaggaaagg
    15361 taaaaatgta tgtgtgtggt ccaacagttt ataactatat acacataggt aatgcaagac
    15421 catttataat atttgatact ctaagaaggt atttagagta tagaggatat gatgtaactt
    15481 atgtacaaaa ctttacagat gttgatgata agattataaa tagaagtcat gaggagggaa
    15541 tatctcctga agaagttgcg gctaagtata taaaagaata ttttgtagac tgtgatgggc
    15601 taggaataaa aagagctact gttcatcctc aggttacaga taatatacaa cagattatag
    15661 agtttataaa agagttggaa gataagggat atgcatatgc agtaaatggt gatgtatatt
    15721 ttgatacaaa taagtttgaa ggttatggaa aactttcagg tcaaaaacaa gaagatttag
    15781 aggcaggggc tagaattgag gttaatgacc aaaaaagaca tcctatggac tttgttcttt
    15841 ggaaggcaaa aaaagaaggt gagccaggat gggatagccc ttggggagaa ggtagaccag
    15901 gatggcatat agaatgttct gttatgtcta agagatattt aggtgaaaca attgatatac
    15961 atgcaggtgg gcaagattta acattcccac atcatgaaaa tgaaatagct caaagtgaag
    16021 ctagaagtgg aaaaacattc tcaaaatact ggatgcataa tggatatata aatataaatg
    16081 atgaaaagat gagtaaatca aaaggtaatt tctttacagt aagagatata tcaaaattat
    16141 atgatttaga gatagtaaga tttttcatgt tatcagcaca ttatagaaat ccagtaaact
    16201 ttagtgatga gatgttaaat caagctaaag caggtcttga aagactatat aatactaaag
    16261 aaaaattaga atttacgcta agtaatttag tagaatctcc tttaacagaa aaagaagtag
    16321 agcttgttaa agaattagat gactttagac aaaaattcat agatgctatg gatgatgatg
    16381 ttaatacagc agatgcagta agtgtaatat ttgaattagc taagttaata aattctaatg
    16441 tagatgaaaa ttcttctttg gagtttgtta aaaaatgctt agatgaattt aatgaactta
    16501 caggagtatt aaatattgta aataagaaaa aggatactgt attagataaa gacattgaag
    16561 aattgataca aaaaagaact gatgcaaaga aaaataaaga gtttcaatta gctgatgata
    16621 ttagacaaca attactagat atgggaattg tattagaaga cacaagacaa ggtgttaagt
    16681 ggaaaaggat ataaaaatgg aaaaaactga attagttact atgtcaccat tagttttagc
    16741 atacttaggt gatacagtgt atgagacata tataagagaa tatttaatta gacagaatac
    16801 acaaagaaaa gttaatgatt tgcataaact agctataaag tatgtaaaag ctaaagctca
    16861 ggcaactata attcatgaag ttgaaattga gttaactgaa gaagagagta aaatttataa
    16921 aaggggaaga aatcaaaagt caaatacttc tcctaagaat gcagatatta tagactacaa
    16981 acatgcaact gggtttgagg cattagttgg atatttgtat ttaaataatg aaatagagag
    17041 acttcaatat attatcaata aaggaatcaa aattattgaa agagatatgt aaatattaaa
    17101 gagtgttgtc ttaataataa gtaataaggc aacacttttt ttataactaa tgtataataa
    17161 atatatagaa attagtaatg agaaaggata aaaagttatg aaagtaaaat taatatcaca
    17221 tacacctgaa ccagaaaaag taatagctat ggcagctaag ctatgttatt caccagttgg
    17281 gacagacgaa atagaaaaag acttaacaga tgaaagtata gagaaatttt taaatatgtt
    17341 attaagtata ggtcatgggt caatattaga acatgcttca tttacatttt caatagaagg
    17401 tatttcaagg gcatgttcac atcaaattgt tagacatcgt atagctagtt tttcacaaca
    17461 aagtcaaaga tatgttaaat tagaacaatt tgagtacata attcctccag aaatagaaaa
    17521 aatagaaaag gcaaaagagt tatttataga ctccatgaaa aaagaccaag agaattatga
    17581 taagttagtt gaaatattgt ttgaaaatca ttataatgac ctaataaaaa acggtaaaaa
    17641 tgagaaaaca gctaaaagac aagctgagaa gaaagcaata gaagatgcta gatacgtatt
    17701 tccaaatgca tgtgaaacaa aaatggtatt tactataaat gctagaagtt tgtttaattt
    17761 ctttgaacat agatgctgtg aaagagcaca gtgggaaata agaaatttag ctgtggagat
    17821 gttaagagag gttaaaaagg ttgctcctat attatttaaa aagactggtc caagttgtgt
    17881 aaatggaagt tgtccagaag gaactatgac ttgtggagat atagttcaag ttagagaaaa
    17941 atttaaggcc ttgtaggagg tgaataattt ggcgagtata gaaggaagaa atccggtaat
    18001 agaagcaata aaaagcgaca gagaaataga taaaatatta attgcaaatt cagctaaaga
    18061 aggttcaatt aagaaaataa taggaatggc taaagataag aatataatta ttcagtatgt
    18121 tgataaacat aagctagatg aagtaagtac aagtcattca catcaaggtg taatagctta
    18181 tgcaagtgag tataagtatt atgaattaga tgagctaata gatttagcaa agaataaaga
    18241 tgaagaccca tttttcatta tattagatga gataacagac ccacataatt tgggttcaat
    18301 aataaggaca gcagatgcag taggagcaca tggagttatc attccaaaaa gaaggtctgt
    18361 acacataact cctgtagttg ccaaagcatc tgctggagca gtagaatata tgccagtttg
    18421 caaggtaacc aatatagtta atacaataaa aagactcaaa gaagaagggc tgtggattgc
    18481 tgcagctgat atggatggag aaacttttta taaacaaaat cttactggtc ctcttggtgt
    18541 agtaattgga agtgaaggtt ttggaatatc tagattggtt aaacaaaact gtgactttat
    18601 agtaaaaatg ccaatgatag gtaatgttac atctttaaat gcatctgtag caggtggaat
    18661 acttttatat gagatattca gacaaagact ggataaaagt aaataggcat gaaaaaaaat
    18721 ataaatcatt acttaataat agatggctac aatattataa atgcgtggga caatttgaaa
    18781 gaactggcta aagaggactt agaagattct cgagaaaagc ttattgatga cattatagaa
    18841 ttttctgagt ttatgggtta taagactata atagtttttg atgcttataa tgttaaaaat
    18901 tctcgagaaa aagttgaaaa aagaaaacac ataacaatag tttatacaag agaacaccaa
    18961 acagcagata gttatataga gaaatttata acttctttat caaagtatga tgatgtaaaa
    19021 gtagccacta atgactatgc agaacagcaa atgattttag gaaaaggtgc tactagaatg
    19081 tctgcaagag aattaaagtt agagttagat cgttccaaga ataaaatgaa ggaaaaaaat
    19141 atcggtttga ggaaaaaaat tcagcgaaat tggttggagg aaaggttaga taaagaaaca
    19201 ttgtcgaaac ttgagaacat tcgtagaaag cgttgaaatt actaggttct ttagactata
    19261 atatacctat aaatttgttt tatatatggg ggagatttac atgttggtag caaaagaaaa
    19321 aagttatgag ttagtggaca attgtcagca ggatgagtat aacattgtat taagagcaag
    19381 tgaaggggat aaaatagcac tagagtacat tattactaaa tacagaaatt ttgtaaaggc
    19441 aaaagcaaag tcatattttt taattggagc agataaagaa gatataatac aagaaggaat
    19501 gataggcctt tataaggcag ttagagattt tgatggaagt aagaccaatt catttaaatg
    19561 ttttgcggag atatgtataa caagacaaat aataactgct ataaaaactg caacaagaca
    19621 aaaacatata cctttaaatt catatgtttc tttgaataaa ccaatatatg atgaagagtc
    19681 agacagaaca cttttagata ttatagctac aagtatagta acagatccag aagagcttat
    19741 aattagtaaa gaagagttaa aaaatataga atcaaaaatg aacgaattgt taagtgactt
    19801 ggaattagaa gtactagaat tatatttaaa tggaaagtca taccagttta tagcggacaa
    19861 gcttaaaaga gatgtaaagt caatagataa cgctttacaa agggttaaga gaaaattaga
    19921 aaaacatctt gaaaacagaa atgattaata gtaaaatact taccataagc ttattgtgag
    19981 caaaattaat ttatatcgaa agataaggga ggaaattcaa aatggctaaa gctaaatacg
    20041 aaagaacaaa acctcatgtt aatataggga caataggaca cgtagaccac ggtaaaacta
    20101 cattaacagc agcaataaca aaaacattat atgacagata tcaattagga gaagcagtag
    20161 atttcgcaaa catagataaa gctccagaag aaagagaaag aggaatcaca atatcaacag
    20221 cacacgttga gtatgaaaca ccaaatagac actatgcaca cgttgactgc ccagggcatg
    20281 ctgactacgt taagaacatg ataacaggag cagcacaaat ggacggagca atattagttt
    20341 gttcagcaac agatggacca atgccacaaa caagagagca tatactatta tcaagacaag
    20401 ttggagtacc atatatagta gtattcttaa acaaatgtga catggtagat gatgaagagt
    20461 tattagagtt agtagagatg gaagtaagag atttattaac agaatatgat ttcccaggag
    20521 atgacactcc aatagtaaga ggttcagcat taatggcatt agaagatcca aagagtgagt
    20581 ggggagataa gatagtagaa ttattcgagc aaatagatga gtatatacca gctccagaga
    20641 gagatacaga taaaccattc ttaatgccag tagaggacgt attctcaatc acaggaagag
    20701 gaacagttgc aacaggaaga gtggaaagag gagtactaaa agtacaagac gaagtagagt
    20761 tagtaggatt aacagaagca ccaagaaaag tagtagtaac aggagtagag atgttcagaa
    20821 aattattaga ccaagcacaa gcaggggata atataggagc attattaaga ggagtacaaa
    20881 gaaacgagat agaaagagga caagtactag caaagactgg atcagtaaag gcacacacaa
    20941 agtttacagc agaagtatat gtacttaaaa aagaagaggg tggaagacat acaccattct
    21001 ttgatggata tagaccacaa ttctatttca gaacaacaga cgtaacagga gcttgtaagt
    21061 taccagaagg aatagagatg gtaatgcctg gagataacgt aacaatggaa gtagacttaa
    21121 taaactcaat agttgtagaa gagggattaa gattctcaat aagagaaggt ggaagaacag
    21181 tagcttcagg agttg
//
LOCUS       DACXMZ010000046        20889 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.046, whole genome shotgun sequence.
ACCESSION   DACXMZ010000046 DACXMZ010000000
VERSION     DACXMZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.046"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            48..869
                     /locus_tag="JD016_06795"
     CDS             48..869
                     /locus_tag="JD016_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAU4999344.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            890..2086
                     /locus_tag="JD016_06800"
     CDS             890..2086
                     /locus_tag="JD016_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAU4999345.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            2253..5648
                     /locus_tag="JD016_06805"
     CDS             2253..5648
                     /locus_tag="JD016_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HAU4999346.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDMGYRSINKSFKDEENSYKLIDGYNIIH
                     NDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSINN
                     TDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINIT
                     GRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDINR
                     AIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNSA
                     MLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGNE
                     GNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKSV
                     GISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYIIT
                     GRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRGP
                     TIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYPN
                     QAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            5658..7331
                     /locus_tag="JD016_06810"
     CDS             5658..7331
                     /locus_tag="JD016_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HAU4999347.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            7604..7798
                     /locus_tag="JD016_06815"
     CDS             7604..7798
                     /locus_tag="JD016_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HAU4999348.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    7967..8200
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8277..9674
                     /gene="asnS"
                     /locus_tag="JD016_06820"
     CDS             8277..9674
                     /gene="asnS"
                     /locus_tag="JD016_06820"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAU4999349.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(9847..11022)
                     /locus_tag="JD016_06825"
     CDS             complement(9847..11022)
                     /locus_tag="JD016_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAU4999350.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            11441..12805
                     /locus_tag="JD016_06830"
     CDS             11441..12805
                     /locus_tag="JD016_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAU4999351.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYFSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            12827..13318
                     /locus_tag="JD016_06835"
     CDS             12827..13318
                     /locus_tag="JD016_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAU4999352.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTHQKIREGSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNINDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            13591..14262
                     /locus_tag="JD016_06840"
     CDS             13591..14262
                     /locus_tag="JD016_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAU4999353.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            14332..15213
                     /locus_tag="JD016_06845"
     CDS             14332..15213
                     /locus_tag="JD016_06845"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAU4999354.1"
                     /translation="MRTTDMKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGL
                     YVGGSTGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEGVELAKFTTDLGY
                     DAISAVTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENE
                     KIIGVKFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRA
                     RQIFELTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKE
                     ATDEMKSRAKEIYRKYF"
     gene            15246..16748
                     /locus_tag="JD016_06850"
     CDS             15246..16748
                     /locus_tag="JD016_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088753.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HAU4999355.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            16878..17330
                     /locus_tag="JD016_06855"
     CDS             16878..17330
                     /locus_tag="JD016_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAU4999356.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DVFVNIVEYRTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEEGDFL
                     PLDGKNNGYVELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            17490..18293
                     /locus_tag="JD016_06860"
     CDS             17490..18293
                     /locus_tag="JD016_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAU4999357.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDKNWFLNDTIEKF
                     NAGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            18351..19238
                     /locus_tag="JD016_06865"
     CDS             18351..19238
                     /locus_tag="JD016_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAU4999358.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEKNEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGKDATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            19257..20105
                     /locus_tag="JD016_06870"
     CDS             19257..20105
                     /locus_tag="JD016_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAU4999359.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            20168..20857
                     /locus_tag="JD016_06875"
     CDS             20168..20857
                     /locus_tag="JD016_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAU4999360.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
BASE COUNT         8194 a         2227 c         3754 g         6714 t
ORIGIN      
        1 aaagtctata tacatttaga aaatactgtt taaaaggagt aggatttatg ataaaaaaag
       61 agatatctaa ctttagagga agtagtgatt atgtagtatc accagaattg atggcatcag
      121 ttaatgttgc tattgctctt gaaaagccac ttttgattaa gggcgaacct ggaacaggta
      181 aaactatgct agcacaagca atttcaaatg aattaaaaaa agatttagta atttggaata
      241 taaaatcaac tacaaaagca caagaagggc tttatgtata tgatactgtt caaagacttt
      301 atgatagcca atttggagga gaaggtgtag atgatattag caagtacata aaatatggta
      361 aactaggaga ggcctttagc tcaaatcaac aagtaattct tttaatagat gaaatagata
      421 aggcggattt agaatttcca aatgacttgc tttgggaatt ggataaaatg gagttttaca
      481 taaatgagac aaaagaaact gtaagagcta aacaaagacc aatagttatt ataacttcaa
      541 atgcggaaaa agaacttcca gatgcttttt taagaagatg catatttcat tatatagaat
      601 ttcctgatag agacatgatg gaagaaatag ttaaagtaca ttttgataaa gtagaagaac
      661 acctattgga acaagtaatg actacttttt attggattag aagtttaaag gacatacaaa
      721 aaaagcctag tacatcagag ttaatagact ggatacaagc attaactttg agtggaatgc
      781 ctattgaaaa aatagaaaaa gaagttcctt ttgcaggaat actacttaag aataatgagg
      841 atattgaatc tatgcaaaga cacttatagt taataggagc tggataatta tgtttattga
      901 atttttttat ttattgaggt caagaggatt aaatgtatct ttaaatgaat ggatgacttt
      961 aattgaagca ttggataaag gactgtgcta ttcaaacttt tcaaattttt actatctttg
     1021 caggatgatt ttaataaaaa gtgaaagtga ttttgataag tttgatgcag tgtttttgga
     1081 gtattttaaa ggtatagaac accaagaaga aattccagaa gagattatga attggcttaa
     1141 aaaaccagat atagatttag aagagtttga aagattagaa caaaatccaa atataaatct
     1201 tgataaatta agagctaaat tggaggaacg aataaaagaa caagattctc ctcataatgg
     1261 tggtaattat tggataggca caggaggcac ttcagagtta ggacattcag gaaaagggca
     1321 aactggtata cgtataggtg gaaattctac ttatggtaga gctgttcttg aagttgcagg
     1381 agaaagaaaa tacagagatt ttagagatga tgaagtttta aatatgcgtc aatttcaagt
     1441 tgcactaaag agacttcgtc aattttctac tagaatagat gctccaaaga cagagcttga
     1501 tttggataag actattgaag agacttgtaa taatgcagga tatttaaagt tattttttga
     1561 gaagccaaga aaaaatacag tgaagctttt attgcttatg gattcaggtg gttcaatgag
     1621 agggtatagc actttatgta atacattatt tcagtctgta agtaaatcaa atcattttaa
     1681 agatgtaaag atatattatt tccataactg tatttatgat agattattta caacacctga
     1741 atgttggtta agtaaatcta ttaatactga atggatatta aaaaatatag ataaaaacta
     1801 taaagttata attgttggag atgcttctat gtcgccttct gagttgcttc atattggtgg
     1861 taactatcgt ggtccatata attacacacc tggtatagag tggcttaaaa ggtttaagag
     1921 aaagtacagt aaaattgtat ggatgaatcc agaacttaga gatggatggg attcaattaa
     1981 ttattggtat caaactcaaa gaatgataca aagtgaattt aatatgtttc cattgactgt
     2041 aaaaggtctt gagaaagcat tgaaaaattt aatggtttca agataaagta aatataaatg
     2101 atggtatgat ttattcaaaa ttgtaccatc attatttatt tttttaatat acctattata
     2161 aattgtataa ctgaaactta aaaaattata tagtctaata cattttagac tcacacaaaa
     2221 catatacatt aataataatt ttagaagtcg aggtgattat aataaattat gaattaattg
     2281 taaaatacaa tggagatata ttgaggttag aagaagaatt aggtgtatca gtagagatac
     2341 ttaattcatc atatgcaata ataacatcaa gtaatgaaga agatgtaaat atacttttaa
     2401 cttatccaga aattgaattt atagaaaagc cttttatatt acagactcag gatgtgcaaa
     2461 gtttttcaag tacaggaata acaggattta aaaatagaac tggtttaact ggaaaaggaa
     2521 ctataattgg tataattgat tcgggaatag attatactct acctgtgttt agagatagtg
     2581 atggaaggtc taagatatta tattattggg accaatctat tcaaggaaat ccaccagaag
     2641 gttttagaga aggaacatta tatactaatg aagacataaa taatgcaata gatggaagta
     2701 tgtatatacc tatttcaaca actagcttac atgggacaca tgttgcaggt atttgtgcaa
     2761 ctattgcaag tgatgccaga ataatagtgg ttagagttgg aaatatacag accgatattt
     2821 tttcgagaag tacagagttt atgagggcaa ttaaatttat tttagatagg gcattagaat
     2881 taagaatgcc tgttacatta aatataagtt atggaagtaa tgaaggttca catagaggaa
     2941 catctttatt tgaacaatat atagatgata tgtgtttatt ttggaaaaac aatatagttg
     3001 tagcagctgg aaataatgca gataaaggag gtcataaaag gattaggctt caaaataata
     3061 ttacagaaga agttgagttt atagttgggg aaggagagcg tatattaaac ataaatatat
     3121 ggccagattt tgttgatgat tttagtgtac atttggtaaa cccatcaaat aatcaaacac
     3181 aagcaatctc tctaacttca ggtgagataa ggaatacttt gggtgaaact agaataactg
     3241 gttattttta ccctatagca ccgtattctc tcacaagaag agttactcta caattaagtt
     3301 caaatacaca gataactcct gggttatgga aaattgtatt tgaaccaata gatatagtaa
     3361 ctggtaatgt aaatatatat ttgccaactt ctgaaggtct aaatagaaat actagatttc
     3421 tgattcctac tcaagagctt actgtaacag ttcctggaac agcaagtaga gttataacag
     3481 taggtagttt taattcaaga acagatatag tatctatatt ttctggtgaa ggagatacac
     3541 aattaggtgt ttttaaacct gatttattag ctccaggtga agatatagta tcttttttac
     3601 caggaggtac aagtggagca ttaactggaa ctagcatggc tacacctcat gttacagggg
     3661 tttgttcatt gtttatggaa tggggaattg taaatggaaa tgatttattt ttgtattcac
     3721 agaaattaag ggcattactt ctaaagggag caaggagatt aagtaatcaa tcttatccaa
     3781 ataattcatc aggatttggt tttttaaatt tatcagatat agatttgtac actctatcta
     3841 atataaatca agatttagaa acagaggata tgggatatag aagtattaat aagtcattta
     3901 aagatgagga aaatagttat aagcttatag atggttataa tataatacat aatgacttag
     3961 agaatgaaat ttatataagt aaaaatgcaa gtagacaatc aggaatacta tcaggaatag
     4021 atatagtcca tactcctgaa tttgaagaag aactagctgg gttaggtatg agtcaaaggt
     4081 tttttaagat aagtgattca ttaggtgtac tatctataaa taatacagac tatagtagta
     4141 tacaacgagt tttgcaactt ccttcaatca taagaactgt atcgactaca aagatgacat
     4201 tacttggaga aattaatcga ggtacttttg gaggtgtagt tgcaacagaa gaaatgggcg
     4261 taaatttctt taaaaataat cctaatatta atataacagg aagagggact ctaatatcaa
     4321 ttgcagatac aggaatagac tatctacacc cagattttat ttatccagat ggcacttcaa
     4381 aaatagtata tttatgggac caaacaaaag aaggaactcc tcctgatggt ttttatattg
     4441 ggacagaata tacacgagaa gatataaatc gagctatcgc agaaaatgac cctagtctat
     4501 ctcaggatga agttggtcaa ggaactatgt tgagtgggat atgtgcaggg cttggaaatg
     4561 taaatagtga atatgcaggt atagcagaag attctgagct aataataata aaactcggca
     4621 agatagatga tttttataat agtgcaatgt tgtttgctgc atctcagtat gcatataaaa
     4681 aagcttttga acttggaaga ccattagtta taaatatgtc tttaggtacc agtagcctag
     4741 caggtttaac caataggagt aatagtgaaa aagcgttctt tactagaggg ttatgtataa
     4801 cggcaggagc aggaaatgag ggtaatacac agacacatac atctggaata ataccatatg
     4861 ttggaggttc tgttgaagtt gaattagagt taaatgaaga cgaggaagaa ttatcattag
     4921 aactttggtt aaatagacca gataaagctg atgtaataat agtatctccc actggtgaag
     4981 aaagtaaaag tgttggaatt tcaaattata ataaagtaac aggcttattt gatttagagg
     5041 ggactgaata ttctataaca tatatatacc caacaacttt ttctggacag caatttacta
     5101 atgttacttt aaagaatgca aaaagaggtg tatggaagat tcgattagta ggagtttata
     5161 ttataacagg aagatataac ttataccttc caaatagaga acttttaaaa agtggaacta
     5221 gatttagaga ggtagaccct ttttacacta taaattatcc tgcgattcaa gatgatttaa
     5281 taaccatagg agcctacaat actattaatg gtagtttatg gcaaagttca tcaagagggc
     5341 caactattga agacaggtta aaaccagata tagtagcacc aggtgtcaat ataatagctg
     5401 cttatcctgg gaacacgtat gcaactataa ctggtacagc agcagcaagt gcacatgcag
     5461 ctggtgctgc tgcaatgtat tttcaatata cttttgttga cggtagatat ccaaaccaag
     5521 catatgtgca aaaaataaaa acttttatgc aagctggtgc tagaaaagat tcaaacacag
     5581 tatatccaaa tacaaattct ggatatgggc tcttggatgt tagaggaatg tttgatgttt
     5641 taagatagga gggatttatg gaaaaatctt attgtataat ttatcaaggt gatatagaat
     5701 ctgccctaca agaaaatgga ataaataggt atatggtttt aaatagtcag cttgcagtaa
     5761 tatatgtacc tttagatttt gatgaaacta ttttaaataa tataatacaa gttgcttggt
     5821 gggaagaatc tgaaccaatg agcagtctaa ttgaaataac taataatgta aataatggag
     5881 aaaccattac aacagcagct gaaactgatt atatttatga aaatccatat aatgatataa
     5941 cagggagagg aattttatta gcagttatag attctgggat agattatttg catcctgatt
     6001 ttataaatga tgatggaact agtaaagttt tatatttatg ggaccaagaa gcaaatacaa
     6061 atccacctcc agaaggtttt atttttggaa gtgaatttac aagaagtgag ttaaatatag
     6121 ccataaatag aaatgatggg agtttgagtc aagataatat aggtactgga actttagttt
     6181 ctggtatatt agttggaaat ggaaggataa attctcagta cagaggtatt actacagaat
     6241 cagacttaat agtagttaaa ttaaaatcat atacagatac ttattatgct ggaaggataa
     6301 attacagtgt ttcagatttt ttagcagcta taacgtatgt tactaacata gcaagaacag
     6361 aaaataaacc actgataata aatttgacta ttggagttaa gtcaagtgct gtagcaacta
     6421 cttcaatact agatactttt aatatattga gtagtgctgg tgtggtagta gtgagtggtg
     6481 caggaaatca agggaataca gatattcatt attctggcag atttagcagt gtgggagaag
     6541 ttcaagatgt aataatacaa gatggagatg actatgctct tgatataaca ctaaatacga
     6601 atggtccaga taaagtggga gcacagataa tatctccttc aggtgaagtt agtcatgata
     6661 taaggtattc tccagacttt tatatatata gaggaaaatt taacttagaa aatactactt
     6721 atgcaatgag atttatttat ccatatatta cctctggaaa agaaaatttg gagataagat
     6781 taagagatat aaaacctgga gtatggattt taagattgac ttcagaactt ataataaatg
     6841 gagaatatga catatacttg ccaaataaaa atttgatagc tccagacaca aggtttttag
     6901 accctgattc agtagctact ataactatgt atgctgctag tgatgatgta attacagttg
     6961 gtacatttaa caataagact gatagtatgt ggattggttc ttcgaaagga ccaattagag
     7021 gaagaggaat taagccagat atagtagctt caggtgtaga tataatatcc acatataaaa
     7081 atggaacata taatacagga acaggaacag gagtgagcag ttctatagtc acaggtgttt
     7141 tagccttatt gatggaatac ttagaaaaac aggataatgt accgagatta tcattgttta
     7201 cacaagtttt aaaaacatat ttaatattgg gtgctacaaa acttgagata tatacatatc
     7261 caaatgtatc gcagggatat gggattttaa atttaaaaaa tacgattcaa cagatagcaa
     7321 atactctata gataagaaac tatgtaaaat tataattgat tattagttta attgtatcaa
     7381 atactttaga tagagtagaa aaatagcata aaaaataaat atgaaaatta aaaaacagtt
     7441 ttatattttg aatacacaaa gtaaatattt tgtatataaa taggtataaa ttaagtattg
     7501 aaaagatatg atgttaattt aaaaataatt ataatttaga cttatgaatt ttatttgtaa
     7561 aattgctaaa actattacaa aataaataat agaggtgata gttttggaaa gtaaaaaggc
     7621 aagagcatta aaaggtaaag ataataaaag acttaaaagt tcacttttag ttggaaatga
     7681 gtcgacagca gcatgggctg atgttgaaaa attaaaacct catagtaaag tttcaatacc
     7741 ttcaattaag ggtgtcgaag aagcaaaaga ctgggttgac aatggaagta aattataaaa
     7801 ttttggaaat tgaagtaata aagaagtaat gaagataagt atttattatc attatgtaag
     7861 gtagaattat tatattttaa ataacaatca gtatcaatta tataaattta ttgactaaaa
     7921 atttataaat tggtataatt ttgttaatta gcagaaataa taaaagataa atactgtgaa
     7981 gagaagagta gatataaaat gtagttctag cgagttgagg atggtgaaag ctcaatacga
     8041 ggtttatatt gaagaacatc ttggagtttc taatcgaaag cttatttagt aagtagactt
     8101 agacggaagg caccgttata ggctttaagc attgcttaac taagaggtca ttatgacaac
     8161 tagggtggta ccgcgagagt aatagtcttt cgtcccttta tatagggatg aagggctttt
     8221 tttattgaaa ttatatttaa aagaaattaa taacaattat actaggagga tttaaaatgc
     8281 aaaaggaatt tataacagta aaagaattgt acagagaaaa agaacagtac atagggaaga
     8341 ctgtaaaagt agctggatgg ataagaacat ctagagtatc taaaaatttt ggatttatag
     8401 agctaaatga tggaagcttc tttaaaaata tgcagatagt atttgatgag aaagtagaaa
     8461 attttaaaga ggtaggaaaa ttagcaataa gctcatctat acttgtagag ggagagttag
     8521 tatctacaga aggggctaaa caaccagtcg aaatacatgc aaaaagcata gttgtagaag
     8581 gggaatcaga tagttcgtat ccactacaaa agaagagaca tacgcttgaa tatttaagaa
     8641 cgatagcaca tttaagacca agaagtaata ctttttcagc tgtatttaga gtaagatcaa
     8701 tagctgccta tgcaatacac aaattcttcc aagaaagaaa ctttgtatat gcacattctc
     8761 caatcataac aggtagtgac tgtgaaggtg caggagaaat gtttagaata acaactatgg
     8821 atttaaatga tattccaaaa actgaagatg gcaaaataga ctatactcaa gatttctttg
     8881 gaaaagaggc taatttaaca gttagtggtc aattaaatgc cgagataatg gcactttctt
     8941 ttagaaacgt ttatactttt ggacctacat ttagagctga aaactcttat acaggaagac
     9001 atgcttcaga attttggatg atagagccag aaatagtttt tgcagacctt gaagataaca
     9061 tggaacttgc agaagatatg ataaaatatg ttataagcta tgtattagaa aatgctcctg
     9121 aagaaatgga attttttaat agttttatag ataaaggatt gttagaaagg ttaaataaga
     9181 tagtaaactc tgagtttatt agaataacgt atactaaagc agtggaatta ttaattgaat
     9241 ctgggcatga atttgagtat cctgttgagt ggggatgtga ccttcaaact gagcatgaaa
     9301 gatatataac ggagcaaata tataattgtc cagtgtttgt tacagattat ccaaaagata
     9361 taaaagcttt ctatatgaga atgaatgaag atggtaaaac tgttagagca atggatttac
     9421 tagttcctgg agttggagaa atcgtaggag gttctcaaag agaagaaaga gaagatgtat
     9481 tgttagatag aattaatgaa atgggattaa gagaagaaga ttattggtgg tatttagaac
     9541 ttagaaagtt tggtactgct actcactctg gatttggtct tggatttgaa agaataatta
     9601 tgtatatgac aggtatgtct aacataagag acgttatacc tttcccaaga actcctaaaa
     9661 atgctgaatt ttaataatta tatataataa aacttatata actttaagta taaacactaa
     9721 aatgacttgc atatttatct attttagaga tatgcaagtt atttttattg tactataaat
     9781 atataatttg attactttta ttttgcgata taatacttat aggtttatgt aatagtatgt
     9841 aaccttttaa taaaatgcaa cacaattacg tccttgttct tttgcagtat atagagcctt
     9901 atcacaatta cttataaact catttaatgt tattgtcttt ccaattttct ttgtataaac
     9961 tccaatacta acagttacac attttaaagg aacatcatca cgctcgaaat tgcaggactc
    10021 tataattttt cgtaaacatt ctgatttaag tttagcatct gcctcattac agttaaaaat
    10081 acatataaaa aattcctcac caccatatcg tatagcatgt tcattttcag aacttgtaaa
    10141 atctaaaatt actttggcaa tattttgtag acaatagtct cccttttgat gtccataatt
    10201 atcattaaat tgtttaaaat aatctatatc tatcatcatt ccagttatag gttcttgatt
    10261 tgcgtgactg attaattttt tttgtagaat atcttcaagg tatctacggt tgttcatttt
    10321 agtaaggtca tctgtcataa ctttgtcgtt aagtaaatta tttatatgag atatttcttc
    10381 atattgttga ctaattaaaa gtttatcata gtaagtaaat acacgatttc tatatagcat
    10441 tgtagaaaca aaaatggcaa gggatgttat aaaaaatgtg ttgacaatat tactaaactc
    10501 attgcctggt atagtttgaa tcccaggtaa gaataaatta agaaacaaaa agcatcctcc
    10561 aaaaactatt actgattctc caaattttaa tactgagaaa gctgcagctg ctaacataat
    10621 ataactaaat acacttaggt catttccacc taactggtca agtagagtaa ttacacaacc
    10681 ccataggcag aaaaaagtca cataaattat ccataaattt atataaaacg tacagcaatc
    10741 ctttttttta ttccaaagat aagaaaatac aaacataaag acagaagata cacaaaatag
    10801 tgttacatac atataaaaat accattggcg tcttggtgtg acaaaagggc ctccttctct
    10861 agctgagatt gatatcatca taattatttg agatattact attattatgc aaatgatttt
    10921 acccgtatga taaagataat tgcagagttc tttctcaaca ttgtctttgt gttttttgtc
    10981 tagaggaaat ccaaaaaatt tttttatttt ttctaagttc atataagcct ccattaaaaa
    11041 taaagcataa aaataagtat tcctttctat cattagatat ttggaatact tcgtttatga
    11101 tttaattata tcaaaatata ttgtgtgtaa caatctaaaa ttgtgataga taaaatttat
    11161 tagataatgt cgagtaactt tattgattaa tactttatta taattaagat ttacagatag
    11221 tttactactg aaaataaaga atagaataaa ttcaaattaa gcataataaa atataaataa
    11281 taaagttcgc attaacaaac tatttaaata tcaagacctg ttaaaaaaca acagcaataa
    11341 acaaataaaa tagataaata tatccaatta aggtataatt aagttttata tgatatagtt
    11401 atatttgata atttacaata gtaaaacttg gagggaaaaa atgggtgaaa agaaaataaa
    11461 taatatgaat tattcacata ataattattc caaaaagaca ggtgaaccaa ctagaaaaat
    11521 aaaaaatagc catatcactc ttaaaagtaa agaagaaaaa atttgttcta gttgcaataa
    11581 agaaaataat actaaggata attactgtaa attttgtgga aatgaacttt atgaaatagc
    11641 atcattaaga ccacttgaaa caaaactaga cttgaaatct aaaataaaag agctttcata
    11701 tcatgcaaat aaaagaggtg tatttttaac aacatttaca accatattta tattgtttat
    11761 tattgcattg atatttaaag ctattataac tattcaattt aatgatatta gttatttaat
    11821 aaatccagca catataatcc tagctctcaa cttgggtcaa attagtgtat ctatgtctac
    11881 catgatgggt tctgggttta taaatgccaa tataggtttg ttgatattac ttataatccc
    11941 tattttggta ttattaatat caaacctaat ttttatgaga aaaagatgta gagattcaaa
    12001 aactgtatta gcaaattctc taggagttgg aattttttat ggattagttt tagctatttt
    12061 atctattttt actaatgtaa aaactagttc acatagtatg ttagaatatg gatatgcatt
    12121 agaatatagt tatgaatttt ttagtgttct tttaaatgga ttcgtattag ggtttatatg
    12181 tacatacata actacttata aaaaaagtta tgaaaaggaa aatatgtatt tctctctttt
    12241 ttcaaattct ataagaacat tcatattagg ttatgtttta gtattagtaa tactattggt
    12301 actaacgata tctgatagta gttacttaaa tgaactagat atgagtagtt attcaaatgg
    12361 attaaattta tttactatac tcccacaaat agctagttat atgtgggctt ttgcaaatgg
    12421 aatttcagta accataataa actcaacagt atcaatgttt actttaagtt cttcatcttt
    12481 atttggagat actaaattaa tgttttatgc aatgggagca ttgtctatgt taatattatt
    12541 attaaatgga tacaagctaa gatttaagta taatacagat agtataagac ctataatagt
    12601 atttagtata tattatgcat ttttgatggg aatattggct ttatttagta catttatact
    12661 agatagtaat attaatttct ttaatacaac taattatgga acaactttaa taatgcaatt
    12721 taaagtctta caagctattg taatttcatt tgtatatagc tttgtgatat ctttaatagg
    12781 atataagcta aattcagctg attaaatatg attggaggga atttctgtgg aaggaaatac
    12841 taataataaa gtagatgata aattttctaa ggttaagaaa aagtttggta gtggaaaatt
    12901 taaagttgaa ttatataaag aaaaatcaaa attaaataga gagattgatg atattcaaga
    12961 aaagaaagca aagatattct tggaaatggg aatactgaca catcaaaaaa ttagagaagg
    13021 ttctattaat gataatagtt ttgataaatt ctgtgatgag ttactagaac ttgataagtt
    13081 aatttatgaa aaaaatatgg aaattaatga aatggaaatg gcagaaagta atgtaacttg
    13141 tgagtgtggt tatgttggaa atataaatga taaatattgt gctgaatgtg gtaaaaaatt
    13201 tgaattggaa aatgaatgtg atgattttat aatttgtggt tattgtgaaa gtgagataga
    13261 tacagaggct gaattttgtc catgctgtgg aagaaaaatt ataatggatt tagaataata
    13321 ttgggaaacc tatgtatagt atacatgggt ttttttattg aaattattta aatggactta
    13381 taacagtagt tatataaaat gaaaataaac accaaaaaaa tgaaataaaa tttcttttaa
    13441 atgtaaaaaa caagctaata tataaaataa atcagtaaaa atatagatgc gagaaaaaaa
    13501 tatgaaaaaa tattgcaaag aaaaagtttt catgttatta ttaatacata aatcaagaaa
    13561 ataaatttca gaaacgaggt aatttaaaaa atgctagata aggtaaaggg aagattaata
    13621 gtatcatgcc aagctttaga aaatgaacct cttcatagtc cttttataat ggggagaatg
    13681 gctaaggctg ctatggaagg tggagctgtt ggaattagag ctcaaggagt tgaagatatt
    13741 attgaaataa aaaaagttac tggacttcct gttataggta ttataaagag aaactatgaa
    13801 gattcagata tatatataac gccaactaaa aaagaagttg atgaactttt aacaacaggg
    13861 tgtgaaatga tagcattaga tgctaccaat agagttagac caaataatga agacttaaaa
    13921 gaacttataa agtatataaa agaaaacggt gttctagtaa tggcagatat ttcaaattat
    13981 gatgaggcta tcaaagcaca agagtacggt gtagattgcg tttcaactac actatctgga
    14041 tatactccat atactaaaac attagaaggt ccagattttg tattaatgga aagattagtt
    14101 aaagatttag aaatacctgt aatagctgaa ggaaaggtaa atactcctca agatttaaaa
    14161 aaggtctttg agttgggtgt tcattcatca gtagtaggtt cagcaataac aagaccacaa
    14221 ttaataacag aaaaatttgt taaagcaatt gaaattaatt aaaaattaat ttataaatta
    14281 aaaaattatt atatttaact atatttgaaa attaaaattg gaggtaaaga tatgagaaca
    14341 acagatatga agggaattta ttcagcatta ttagtttcat ttgataagga aggaaatata
    14401 aatgaaaaag ggttaagaca aataattaga cacaatatag atgtttgtaa agtagatggt
    14461 ctttatgtag gaggaagtac aggggaaaac ttcatgcttt caactgatga aaagaaaaga
    14521 atatttgaaa tagctaaaga tgaagtgaaa gaagaaataa aattaatagc tcaagttggt
    14581 tcagttaatt taaaagaagg tgtagaactt gctaaattta caactgattt aggatatgat
    14641 gcaataagtg ctgttacacc tttttactat aaatttgatt ttgaagaaat aaaacactat
    14701 tataatacaa taataaattc tgtagataat agattaataa tatactctat accattttta
    14761 actggtgttg atatgagttt agaccagttt ggagaattat ttgaaaatga aaaaataata
    14821 ggtgttaagt ttacagcagc agatttctac ttattagaga gaatgagaaa aacattccca
    14881 aataaattaa tatttgcagg atttgatgaa atgatgttac cagctacagt tttaggtgta
    14941 gatggggcaa ttggttctac attcaatgta aacggtgtca gagctagaca aatattcgaa
    15001 ttaactaaga atgaaaaaat atcagaagca cttgaagtac aacatgtgac taatgattta
    15061 ataacagata tattaggaaa tggcttatat caaacaatta aattactact tgaagaacaa
    15121 ggtgttgagg ctggatactg tagacaacct atgaaagaag caactgatga aatgaaatca
    15181 agagcaaaag aaatatacag aaagtatttt taataaaata cttttagtat gggaggatta
    15241 gtcaaatggt aggatttact tggatagatt ttgcaatact agtggtttac ttattggcag
    15301 ttttatttgc aggattgtta ttttcaaaaa aggaaatgaa gggaaaagag ttcttcaaag
    15361 gtgatggaac tattccgtgg tgggttacat cagtatcaat atttgccaca ttattaagtc
    15421 caatatcatt tttatcatta gcaggaaatt cttatggtgg tacttggatt ttgtggtttg
    15481 cacaattagg tatgtttata gcaatacctt tgacaataaa atttttctta cctctatata
    15541 gtaggttaga aatagataca gcatatgaat acttagaaat gagatacgaa agtaaaggtc
    15601 ttagagtact tggagctttg atgtttataa tatatcaaat aggacgtatg tctattataa
    15661 tgtatcttcc atcaatagtt ctggctggtc ttacaggaat acctgtaaat gtactgataa
    15721 tagttatggg tgtaatagca ataatttact catatacagg tggattaaag gctgtcttgt
    15781 ggaccgattt tatacaagga atggtattaa tagtaggtgt tacagggaca ttgttctatt
    15841 taatagcaag tataaatggt ggttttggaa cagtaatgga tactcttaca actggacaca
    15901 agttcttagc aagtaatgaa gttatgtttg ataaaaatat actatctaca agtgcattta
    15961 taatatttgt tggagctggt cttaatactt tttcatcata tgtatcgagt caagacatag
    16021 ttcaaagatt tactacaaca actgatataa agcaacttaa taaaatgaca tatggaaatg
    16081 gtgttttatc aatgggactt gcaacagtat tttatctaat tggaacttgt ttatttatat
    16141 actatactca aaacccagat ttagctcaaa cagttcaaca agaccaagta tttgcatcat
    16201 atatagctta tgaattacca gtaggtataa caggaatact acttgcagcg atatatgcag
    16261 catctcaatc aactttatcg acagggctta actcagttgc tacaagttgg actttagaca
    16321 tacaaactat cataacaaaa gatataagta tggaaaaaca aactaagata gcacaatata
    16381 tatcattagg tgtaggtata ctttctatag ctgttgcaat agtattagca aatggagaaa
    16441 taaagtcagc atatgaatgg ttcaatagtt ttatggggct agtactaggt gttttagcag
    16501 gaatatttgt acttggtgca ttttgtaaaa aagcaactaa aatgggagca tatgttggat
    16561 tcatagtatc agcaatttta gttgtttatt taaaataccg tatgccagaa gtaacttctt
    16621 gggcatattc tctaataaca atcacgactt ctgtagtggt aggtacaata gttagtgcaa
    16681 tagaagctaa agttaaagca aaagtatctt taccaaaagc tgagactact gtttattatg
    16741 aaaaataata tttaaatgat aaattgtctt atagagtgat tagataaata atttagttgc
    16801 tctataagat tgtataatat actttgtata taaaagtata aaaatagact ttataattat
    16861 atagggagat agtgcttatg atatttggaa atataaatca tagtaagaca tattcaacat
    16921 tgcatgaaga tttacttaga tgttttgaat atgctaaaga taattcatta atagattatg
    16981 aaaaaggtac ttatacaata gatggtgatg atgtttttgt gaatattgta gagtatagaa
    17041 cttgtgaaaa agaagataga ttctgggaag ctcataagaa gtatatagat gttcatttga
    17101 tgcttgatgg atgtgaacga atagatataa attttataga aaatttagaa caaaaaatat
    17161 atgaagaaga gggcgatttc ttgcctcttg atggtaaaaa taatggatac gtagaactta
    17221 gaaaaggtga ttttttagtt tgttatccag aagatgctca tatgacagga ataaaagttt
    17281 tagaaaaaga aaatatcaaa aaagctattt ttaaggtttt ggttagataa tgataattta
    17341 tataaccact agggctgaaa atagtgattt aactttcagc gattagtgag acttcgtaaa
    17401 ttttagttag attactattt tcagccttag ttttttatga gaaaattgaa aataattaaa
    17461 agcttaaaat tttgtaaagg agttaactta tggaaaatat agatgtattc ttattaatag
    17521 gacaaagcaa tgctagagga ttgggaaatc caaaagagag catcattcca aatgaaaatt
    17581 gttttgaata tttgagtact gatgaaatta taaatatgag atgtgagcta gagacttcag
    17641 aaggtgatgg cactatagca cctgcttttt caaatgaatg gaataagctt actggaaata
    17701 aagtttgttt tattcacaat gcaaaagatg gttcaagaat aaaaaactgg aatcatgata
    17761 aaaactggtt tctaaatgac actatagaaa aatttaatgc aggctgtact accttaagta
    17821 aacattataa tatagaaaat aaatacgtta tttggattca aggtgaaagt gatgcaaaat
    17881 atggtagtga tgtattgtac tataaagaaa gtctaaaaaa aattgcgtat aggcttaaag
    17941 aagaatgtat gattgataaa atgtttgtaa gtttaactgg ttattggctt ggagaagatg
    18001 aatattttat tagaacaaga cgaattgcag cagcacagga gtcggcttgt agtgaatgtg
    18061 atatattatg tgtaggaagc aaaattgcta tgcagtttca tgacagagga ttaactattg
    18121 atgatgttca ttacactcaa gaaggtctta atatgttagg agaggactta tgtaaaaata
    18181 tttataagta tcatataacc aaagaaaaaa gtgtattaag tgatactata gacttatcag
    18241 aagcaaggaa atatatctgt gaattggaaa agataagtaa aagatttgtg taatataaaa
    18301 ttttaaatag agttgttaat ttataaaaat aatttgtatg gagatatgtt atgaaaaatt
    18361 acgtttgtat agatgttgga ggtacttcaa taaaatatgg ttttattaga gaagatggat
    18421 ttataattga taaatcaagt cttgatactg aagcaaagga aaaaggtgga gaaggaatat
    18481 tagccaaaat aaaagatata gtaaaaaaat atatagagaa aaatgaaata agtggtatat
    18541 gtatatcaac tgcaggtatg gttgacccag ttgaaggtaa aatactattt gcattagaag
    18601 aattaattcc aaactataag ggcatgcaat taaaaaaaga agtagaaaaa gagtttaata
    18661 taaaatgtga agttgaaaat gatgttaatt gtgctggact tggtgaaatg tggcttggag
    18721 caggtaaaga tgcaacatct tctatatgtt taacaatagg tactggaatt ggtggatgta
    18781 ttataataaa taataaactg ataaatgggt ttagtaatag tgctggtgaa gtaggatata
    18841 tgaatgtaaa tggaagttct tttcaagaat tggcatctac tagcagtcta attaaaaaag
    18901 tagcaaaaat aaaaaatttg aatgaaaatg acttaaatgg aaaaataatt tttgatttag
    18961 caaagaataa tgatgaagat tgtttaaaag aactcgataa tatgataaaa tcattagcag
    19021 ttggaatcgc aaatatatgt tatgtaataa atccagaagt ggtaatttta ggtggtggga
    19081 ttatggcaca agaaaagttc ctaaaaccaa aaatagacaa agcattaaag gaggttctta
    19141 tagagagagt ttacaaaaac actaatatag aatttgcaaa gagacaaaat gatgcaggaa
    19201 tgcttggagc attatataat tttttaaaca aaatataaag aatagaaggg tttttaatga
    19261 gtattttaga acaattggaa tcaccaactt ttaaagtcac aaaatcagat aaattattaa
    19321 tagcttatat aaaagaaaat atagatgatg tattttataa accaatatca caaatagcta
    19381 aagagagcaa tattggtgaa gcaactataa ctaggtttgt aaaaaaaatg aattttaatg
    19441 gattacagga ttttaaagtt actttagcac aagaaatatc aactatcaat aaaaaaaata
    19501 ttataaataa aaatatacaa aatgatgagc cagctttgga tacagcaaaa aaacttttaa
    19561 gttcaaatgt aaccacttta gagaacacag tagagataat caatagtaag gatgtacatg
    19621 actgcgcaag gcttataata aatgcaaaga aagtttactt cataggtatt ggatattctg
    19681 gtataatagc acaggattca aactataaat ttatgagaat aggtcttaat tgtgtaagtt
    19741 ttgacagtag tcatacaatg ataatgatgt cttccataat ggaagaagga gatttaataa
    19801 ttgcaatttc acattctggt gaaacagagg agattataaa aactgtaaag cttgcaagag
    19861 ccaataatgc taaaattata tcaattactg aaaataaaaa ttctgaatta aaggatattt
    19921 cagatgtaca cttatcttat gtatcgggag aaacagtact ggaaaccggt tcaatatctt
    19981 ctaaattagc acaatttttc attattgatt tagtatatac acaggtagta aaagaacttt
    20041 ctaatgaagc tattgaaagg aaaattaaaa ctacaaatgc tataaaattg tttaaaaatg
    20101 aataaaataa gaaatttgta atgttttata gagctctctt aaaaatttag gaatggagag
    20161 atattacatg tctaaattaa aagattataa tattgataaa gtaataattt ttaataattt
    20221 aagtactaaa acgaagaatc aacttaaaga aaaagttaaa ctaaaaagat tacaaaaaaa
    20281 agaaatttta ttttatgaaa aagatagctt agacagtgtt tatatacttt tagaaggaaa
    20341 ggtatctatt tttaagataa gtgaaaatgg agagagaaaa gtaattttca tattaaatag
    20401 tggggaagtc ataaatgata ttacacttga cgcttctaaa aactctactg ttggctgtga
    20461 agcatttgaa aattcaattg tagcttatta tagtattgaa gaatttttag aaattatgca
    20521 aaatgatttt caacttacaa aggatattat ttcttatatg gaaagaagaa taagacgttt
    20581 atatagacag ttgaaaaatt ctatatccat aaaagtagat aaaaaacttg cagcaaaatt
    20641 atataggtta agtagagaat ttggagtttg ctgtggagaa tggactttac taaatgtaaa
    20701 tattacagtt acatatttag ctgatatgct tggctgtaaa agggagactg tttccagaat
    20761 gataaaggta ttgcaaaaag aagaacttat taaaattgac aataaaggtt tttatgttaa
    20821 agaaactgaa ttatcaaggt attttaaaaa taattaatta tgattaagta agagtattat
    20881 tagaatttt
//
LOCUS       DACXMZ010000047        20735 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.047, whole genome shotgun sequence.
ACCESSION   DACXMZ010000047 DACXMZ010000000
VERSION     DACXMZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.047"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            90..1967
                     /locus_tag="JD016_06880"
     CDS             90..1967
                     /locus_tag="JD016_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAU4999361.1"
                     /translation="MVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTG
                     AYEGTINYNQDPEYLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIE
                     DITSRKDVNKYLRKTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGI
                     ISTPLATTYNAPILLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTV
                     NASQTRLNGSNRYETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPI
                     ILTDKDSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGAD
                     RHETNANVIKKFYTDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYH
                     KDNLEAKSANKVYKIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGS
                     APGNSSGGMIEKDYNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPD
                     LFTSIHYNGSTNKQGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRV
                     LPSDSTKDYLYVLRSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSL
                     K"
     gene            2209..4122
                     /locus_tag="JD016_06885"
     CDS             2209..4122
                     /locus_tag="JD016_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999362.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDGISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSVFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     SKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKE
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDDIDEHSQYREILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            4115..6334
                     /locus_tag="JD016_06890"
     CDS             4115..6334
                     /locus_tag="JD016_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAU4999363.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            6443..7273
                     /locus_tag="JD016_06895"
     CDS             6443..7273
                     /locus_tag="JD016_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HAU4999364.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            7263..10730
                     /gene="addB"
                     /locus_tag="JD016_06900"
     CDS             7263..10730
                     /gene="addB"
                     /locus_tag="JD016_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HAU4999365.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRISKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRY
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            10730..14557
                     /gene="addA"
                     /locus_tag="JD016_06905"
     CDS             10730..14557
                     /gene="addA"
                     /locus_tag="JD016_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAU4999366.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            14603..15814
                     /locus_tag="JD016_06910"
     CDS             14603..15814
                     /locus_tag="JD016_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAU4999367.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            15801..19328
                     /locus_tag="JD016_06915"
     CDS             15801..19328
                     /locus_tag="JD016_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAU4999368.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDELEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            19459..20712
                     /locus_tag="JD016_06920"
     CDS             19459..20712
                     /locus_tag="JD016_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAU4999369.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
BASE COUNT         8998 a         1958 c         3686 g         6093 t
ORIGIN      
        1 caatcgtcgc acctacaatg gcatttgcta ctgaatctaa tgctatggaa aataacgctg
       61 atttaaatat aaacttagag aaaaaaagta tcgttttagg tagcaaatca aaagttagtg
      121 tcaaatttaa agaaaaacca gatgcagata gcattacatt aaagtataaa tgctatgaca
      181 tgccattgaa tacaactcta aattacaatc aatcaactgg ggcatatgaa ggaactatca
      241 attataacca agacccagaa tatctaaatg tttgggaact acaagggata acaataaaca
      301 gcaaaaataa tcctaaaact ttaaacagac aagacctaga aaagctggga ttaaatttaa
      361 aagactataa tgtaacacag gaatgtataa ttgaagatat aacttctaga aaagatgtaa
      421 ataaatattt gagaaaaact tcttcaccta ttacagaact tacaggaagt gatagatatg
      481 aaacagcagt taaaataagt aaagagggct ggaaaaatgg ttcagataaa gtagttataa
      541 taaatggaga tgtaagtata gatggcatta tatcaactcc actggcaacc acatataatg
      601 caccaatact tttggttgaa aaaaacaatg tgcctaatag tgtaaaatca gaattaaagc
      661 gcctaaaccc taaagatata attataattg gagatgagaa tgctatttct aaaactactg
      721 ctaatcaaat taaatcaact gtaaatgcta gtcaaacacg tttaaatggt tctaatagat
      781 atgagacatc tttattgata gcaaaggaaa tagataaaaa tcatgatgtg gaaaaagtat
      841 acataacaaa tgctaatggc ggagaagtgg atgcacttac tatagcagca aaagcaggtc
      901 aagacaagca accaattata ttaactgata aagatagtat tacagacaat acatataaat
      961 ggttaaagag tgaggattta caaaatgctt attttatagg tggtcctcaa atgatatcaa
     1021 caaatgttat aaataaggta aatggaataa ctaaagatag tgttactaat aatagagtat
     1081 acggagcaga tagacacgaa acaaatgcaa acgtaataaa aaaattctat acagatgatg
     1141 agttagaggc tgttttagta gctaaatcag atgtacttgt tgatgcttta gcagcaggtc
     1201 cattggctgc gaacttaaaa tctccaatac ttataacacc aaagacgtat gtatctgcat
     1261 accataaaga taatttagaa gctaaatcag ctaataaggt atacaaaata ggaggaggat
     1321 tgacttctaa ggtaatgagc tctatagcat catcattatc taaacacaat acgactccaa
     1381 cagaaccagg aaatagtggg ggcaagacag ttatgattga cccagggcat ggtggttcag
     1441 cacctggaaa ttcatctgga ggaatgattg aaaaagatta caatttaaat acttcacttg
     1501 caacaactga atatttacgt tcaaagggat tcaatgtaat aatgacaaga gacacagata
     1561 agactttatc tcttggaaat agaactgctc tatctaattc attgaaacca gatttattta
     1621 caagtataca ttataatggc tcaactaata aacaaggtca tggtgtagaa gtattttata
     1681 agcttaaaga taaaaatgga gggactacta aaactgtagc taccaatata ttaaatagaa
     1741 ttttagagaa atttaaactt acaaatagag gtataaaaac aagagtactt cctagtgatt
     1801 ctacaaaaga ttatttatat gttttaagaa gtaatgatat gccagctgta cttgtagaat
     1861 gtgcattttt ggataatgaa aatgatatga gtttaataaa ctcatctgca aaagtaaaag
     1921 aaatgggtac acaaataggt aaaggaatag aagattcatt aaaataaatg gaaataaaaa
     1981 tatttaaaaa cttaatgtaa atttatagtt gtaaatttat actaaagtca aaaaatgaaa
     2041 tataagtgta tcaatagtaa gactgtatgt acatttgcac atgtgaatgc aagtatatat
     2101 acagtctttt tttgtagtta aatgcaataa tttgattgtg ttttgtaaaa tacacaatat
     2161 gatataatta gataatatac acttaattta attgaggaag gtgattatat ggaatattta
     2221 tttgaaaaaa gtagtttaaa agaatatgga aatgaagctt tagataaata tagagaatta
     2281 ttagataaaa ctggtaataa ttcaaggtgt attcttttgt tagtaccaaa caataacaca
     2341 aagatacgat atgaaagggc tctaagatta aattatagtg aagaactgaa aataacaaca
     2401 tatataagtt ttgtgaagaa agaattagtt aaatactggc cactaataat agaaaaatgt
     2461 gatggaataa gtacaaaggt tgtatctcca acatttatat caaatagttt aagtgaatat
     2521 ttaatagtgc aaaaagttaa ggaaaaaagg atacaagaag gatattttga agatattaca
     2581 ggaaccaata aatccatagc aaatagtata atgactaaca taaataagtc tgtatttaat
     2641 ttaattgatt ttgattttat tggagaaaaa atatattcat caaagaaaaa taaagatagt
     2701 atatatagat tttcatacac tcaaatggat gaaattatat catattatat aaattcctta
     2761 ctatcaaatt caattttaga taatgcatta agcatatatt tatacaatca atacctactt
     2821 aatgatgatt tttatttaaa aaacttattt acagaagtga gatatataat tgtggatagc
     2881 cttgaaagct caagtaatgc agaagttgat tttataacag aagcattgga taatacatta
     2941 caaagttata tatattttga ttatagtagg gattattctg tatttaataa tgtggatata
     3001 gactatataa atgagaaaat aatatcaaaa attaagtcta aagaagaaaa tattaaaaag
     3061 aaggagatta gaatagaaga tttatactta ttacctgcaa atatagaatt aaatcaatca
     3121 agtcaattat ataatgaaat gttagattta ataagtgaaa aagttatatc tcttatagaa
     3181 gaagatgtat ctccaagaga tatagcaatt atatctccta taaacaatag tatattggag
     3241 catcaaatta gagattcttt gatagaaaaa aatatagatg tttttagtac taagaaggat
     3301 aaaaaagctg tggattatcc atatgggaat gcattggtag tggctacatg tatattttat
     3361 ggatatttag attttataaa agaggatgaa tacttaagct ttttagaaac tcttttagaa
     3421 ataaatagga taaaagcatt taagattttt aatgaaacaa gacatttaag tattgatgat
     3481 atagatgaac atagtcagta tcgtgagatt ttacagtata ttgaagaaaa aaagaagagt
     3541 gatataaaaa tacatgaatt tttaactcaa ttttacatag ataaaatgtt aaatttaaaa
     3601 gaaggtaaaa agaatgttgg gttatgtaaa aagataatag ctgaaagtga gtcttttagt
     3661 gaaagtttaa gtctacttgg gatgaaaaaa gaaaagatat ttgtagaagc tttaaaaagt
     3721 acaataaacg attattactc tgttgtcgat atagaagagt tgaaatccaa agacaaaata
     3781 gtaataacta ctccttattc atatatatca tcaaatatag atagaagtat acaaatatgg
     3841 gtagatatag gaagtaatgc ttggaatatg aaaatagaaa aagatataag taatttaatt
     3901 gtacttagaa aatcttttga agaaaaaaag atttatacaa atgaaatgga agaatattat
     3961 aaaaaatact atctatacaa tacagtatat aatttgttat taaatgcaaa gaaaatctat
     4021 gcatttaaaa gtgaatatgc gattaatggt tatatccaag agagtatatt atatagtata
     4081 cttttaaaaa tctcacataa aggagataaa aattatgatt aatataaatt atagagaaga
     4141 ccagatacca ataataaatt acgataatgg tactatggca gtaccagcag taccaggtgc
     4201 tggaaaaaca tttataataa caaatttagt agctaaacta ttacttgaac agaaacataa
     4261 gggtggtaaa atattaattc ttacatatat gaatagtgct gtaaataatt ttaaaggtag
     4321 aataagaaag attcttgaag agaataaaat agatgatact aattcatatg aagtaatgac
     4381 tatacacagt ttggctgtaa aaataataaa ggaaaagcca gaagttgtaa tgttaagtga
     4441 ggattttaac atagctgacg atttgcaaaa aagtattata ttaaatgaat gtataagtag
     4501 atttagattt gaaggtggag aaagagcttt tagatggttt ttaaaggagc aaaaagatga
     4561 acgctggaaa gaaataactc ttgaagcctg ggaaagaggt ttttttgaat ttgcaggaaa
     4621 tgcaataagt gaacttaagt ataagggaat ttctccagat aaacttgaag aaatattgtc
     4681 aagaggacac aaaggaatgc ttaaaattat tttaccaata tataaattgt atgatagaaa
     4741 attaaagcaa aatggattgc tagattatga tgatatatta attttagcag aaaaaacttt
     4801 aagcttagac gaagggctta gaaagaagtt tcaaactaga tacaaatata tatttgaaga
     4861 tgagtgtcag gactcaaatg aaattcaagg taatataata aaaattatat ctagcgaaaa
     4921 taaaaaccta gttagagttg gagatataaa tcagagtata acaggaacat tttcatcatc
     4981 agaccctaaa ttttttaaag aatttataaa tagtgcagat ttctgttata ggatggatat
     5041 gtcaaatcga agttcaaaag atatacttga tttagcaaac actcttgtca agtatgttac
     5101 aaaggaattt agacagaaag aatgtagaca tgctcttgaa gatatgcaaa taaaaactgt
     5161 gccaaatgga atgggatata aagaaaaccc taatccagaa cattataata taaatgtaaa
     5221 gtggtatgag agttggaaaa ttgaaataga gcaaactgct agatatgtta agggtataaa
     5281 aaataaatat ccagataaaa gtataggtat attggttcca tttaatgaac aagtgacaca
     5341 agttgctaaa gaacttagag agaataatct aacctttgaa gaattaggac caaattcttt
     5401 gaataaaagg aaggttataa acaacatagc atatattatt gattttatgt tgcattgtga
     5461 tgatgtagaa aagttaatag ttgtacttaa taaagttttt atagatactg ataatgaaga
     5521 agaaataaag tattttttag aagaattaaa aaactattct accgaagaaa ttatttataa
     5581 ttttgaattt agtaagttag ataatgattt aatagaattt aaagaagaaa gtgatatata
     5641 cttaagtttt aaaagaggaa tagatattat aaaacagata ttagaatatc caataataag
     5701 gttagacctc ttagttttgt ttattgggaa aaaacttaat ttagaaaaag aagataaagc
     5761 tgtactggat tatatatctt tttatataaa gtatctagta tcagaaaata taaatatgga
     5821 tttagaagat gtatataatt tattgtttga tgtaaagaac agggtgttta atcatataat
     5881 agatgtggta tatgagataa atggatatga accagagccc ggtagtataa cactatgtaa
     5941 ctatcataaa tcaaagggga tggaatggga ttgtgtgttt ttactaggtc ttgttgaata
     6001 taatttccct gacaatatta atcaaaagtt tcagagtgat aaatggtatc taaaagaaaa
     6061 atataaaaac cctatggcaa ttattaaatc agaagttgaa gctatattaa aaggaagtat
     6121 ttcaacagat tatgctcata aaacgaagat tgattctata aatgaaaaga ttagactttt
     6181 atatgtaggg attactagag caaaggaaat gttagtactt tcaggaagtg cttatagaga
     6241 tgaaagtgat atagggaata agagaaaaga gcaaaaacca tgtatatatt taagcagact
     6301 taatcaacat attattgaaa aaaggtcaaa ttaatatata ataagttatt aaaagattgc
     6361 ttgaaatata tctaaaaggg gataaaacaa tatgtacagt aagtaactac taaaaaataa
     6421 agttaagaga gaagtgatat gagtgaacga taaattaaaa tatttttctt atagtcaaaa
     6481 ttcaattaac acatataaat catgtccatt gaaatttaaa tataaatata ttgatagaat
     6541 aaattggaaa aatgatgatg ttggtagtag agagtattat gaaactttaa aaacaggaag
     6601 agattttcat ttgatttgtg aaagatattt tagcaatata cctcttggaa tctattttaa
     6661 tgaggatgat aaaaatagta aaaagttttt aaagtggatg gaaaatataa aaaaagttgt
     6721 tcctattgaa aaaggaaaaa cgtatttgcc agaatatgaa gtaaggatga cattaaatgg
     6781 ggatataata caggcaaagt atgatttagt tgtaattgga aataacaata tagaaatatg
     6841 ggattggaaa accgagtcta aaaagattga ctataaacac gtagaaaata gaatacaaac
     6901 tattgtttat atgtttttag caaaagaagt aatacctaaa gttttaaaaa tggacataaa
     6961 tgttaaagat ataaatatga gatattatca acctgaattt gatgacttac caattaccat
     7021 tttatatgat gaagaaaaac atgaagcaaa tagaaataaa attcaaaatt atataagtat
     7081 gataaaaaac actaattatg aagaacatac atacgaagac aatctataca atgatataga
     7141 aaaggtatat aataaagaag aacgtatgtg ctatagaaat aaaaagcatt gcaagtattg
     7201 tgaatttaat aagttatgta atggcaaaga aatagattat agtattttgg aggcggaaat
     7261 ttatgggact taggtttgta ttaggtagaa gtggaagtgg aaaatccacc tatatactag
     7321 atgagattaa aaaagaagct caaaaaaatg aaacgacatc aattatactt ttagttccag
     7381 aacagtacac atttgaggct gaaaatagaa taagtaaact atttttagga aaagaaaaag
     7441 ataagtatct aagagttagg gtacttagct ttaagacact aagtaatata gttttttctc
     7501 aagtaggagg attgacagat gtaaatataa attctagtgg aaaagcaatg atggtatata
     7561 gagctattga agatgtaagt gaagaattaa atgtgttttc aaaatcaaaa tcgcagtctg
     7621 gttttgtaag ctcaattaca gatatgatat cagaaatgaa acaatacaat ataagccctg
     7681 aaatgttaga aaatatatct ggagaattgg ataatgaaac tttaagttta aaattaaaag
     7741 atataagtaa aatatataat tcttttgaag ggaaattgca tgaaaattat gtagatgctc
     7801 aagatatgtt aacttcattg gcaagtaaaa ttgaattaag cagttattta gatggagctt
     7861 gtgtttatat agatgaattt acaggtttta caccaaatca gtacaatgtc ataaagagta
     7921 tattaaataa gtctaaaagt gttaatatct ctttgactgt agatgatatc aactatattg
     7981 ggtacagtaa atctgatatg ttttccagaa cgaaatttac ttattcaaaa ttgactcaat
     8041 tgtgcaacga agaaggcata aaaatattgc cacaagtaaa tttaaacaca ggtgttataa
     8101 aaagatttga aaaagttaag gaattacagc acttagaaag attttataat gcttatccat
     8161 ataaaatata ttcaaatcca actgaaaata taaagataaa agagtttaac aatttatatt
     8221 cagaggtgga agagatagcc agagaaattg tacatttagt tagagataag aatgtaagat
     8281 atagagatat aaccattgct acaagagatt taaatagata tgatttttta gtacactcta
     8341 tatttaatga atataatata ccaaacttta tagataagaa gagagaggca aaaagtaatc
     8401 caatagttat tttaattatt tcagctttag aaatgaagaa tagacgatat gggtatgaaa
     8461 ctatgtttag gtatttaaaa agtggactta taggaattga taatgatgat ataaatttac
     8521 tagaaaatta tgtgttggct aatgggatta aaggtaaaaa atggtttgat gaaaaatggg
     8581 attatagaat aacccaaagt ctttcaggtc aagaaagtga atttgaactg gaattaaaag
     8641 agaaaataaa tgaaataaaa aatagagtat tagagcctat agtaatttta caagaaaaat
     8701 taagaggaaa aaatagagta aaagaaatct gtagatatat atatgaattt ttattagata
     8761 taaatatgcc agaaactata gaaagtttaa tagtaaattt taaggataaa ggggaacttg
     8821 atgttgcaaa tcaatactca caagtttggg acatagtagt ggacatatta gaccaaatgg
     8881 tggaacttat gggtgatgaa ataatttctt tagaaaaatt tataaaatta ataaccttag
     8941 gttttgatga atatgaatta ggactggttc ctcctagtat agaccaggta cttgtaagta
     9001 gtgtggatag aatgaaaaat ccagacacga aatatttata cctagttgga actactgatg
     9061 gtgtgtttcc gctgattaca aaggatagtg gaatattgag tgataatgat agggaaagtc
     9121 ttggaaacaa aggaatcgaa gttgatattg atagtaagac taggagtttt gaagaacagt
     9181 ttcttgtata taaagctctt acttcgacat ctaagaattt aaccataaca tatccaatat
     9241 cagaccatga aggtaaaacg cttagaccat caataattat atcaagactt aagaagatat
     9301 ttccaaatat agaaaataaa agttatttag tggaagaaaa taaaagcact gataaagata
     9361 tacttaaaaa aataacggtt aaatccccta catttaatga acttataaac gtaataaaaa
     9421 attatgacag tgatggttac aatacagaag agattaatag tatttggtta gatatatata
     9481 ggtactattt aaaagatgaa atctatagtt ctataacaaa aaaagttata aaaggtctta
     9541 gttatactaa tcaagttcat aagattgaag agaaaaaaat aaggtcattg tataaaagta
     9601 attctcttag catatctagg cttgaaaaat atgctgaatg tccatttgct tattttatac
     9661 aatatggtct aaaagcaaaa aagcgtaaag agtattcatt tacaccacca gatttaggaa
     9721 cttttattca taatatttta gataggtttt caaaagaact tttacaagat aatttaactt
     9781 ggagagatat tgatgaaaaa tatatagaat taaagatagg tataatagtt gatgaaataa
     9841 ttttaaagat accaggatat atattaaata gttctgagag gtataaatac cttgcatata
     9901 gattaaagaa tatgttgaca actgctataa ctataattag tcagcaaata aaacaaggtt
     9961 catttgaacc aatagattat gaagttaaat ttggcgacaa tggaaaatat ccacctataa
    10021 aaatggtgtt agaaaatgga caagaagtaa gtttaattgg acaaatagat agagttgatg
    10081 aatttgaaga aggagaaaat aaatatataa gaataataga ttataagtca ggcaataagt
    10141 ccataagtct aacagaaata tattatggtc tacaattaca attacttgtg tatttagatg
    10201 caatccttga aagtgcaaag gatgaagata taaatataaa tccagctgca atactatatt
    10261 gtagaataaa taatccaata gcaaaattta atgaagataa agatgatgaa gaaatacaag
    10321 aagcaatatt aaaagaactt agaatgaagg ggcttgtagt aaaagactct cacatagtta
    10381 aagagatgga taaatcacta atagatggag aaaggaaaaa ctctttagta ataccagtag
    10441 gtcttactaa ggatggaaat gtaggtaaaa gtacttcagc aataagctat gaggatttca
    10501 aactgcttag aaaatatgta aggtatgcta taaaggattt atgtgaagaa atgttaagtg
    10561 gagaaatacg tatagctcca tataaacata aagatggaac atcatgtgac ttttgtgatt
    10621 actctgctat atgtcagttt gattcaacta taaaagataa caagtataaa aatttaaata
    10681 ataagagtaa tgaagagata ataaaaatga tgaaaggaga tgttaattag tgagttctcc
    10741 taaatggaca aaagagcaat tagaagttat agaaagtaga gaatgcaatt tattggttgc
    10801 tgctgctgct ggttcaggaa aaacagccgt gctagttgag cgtataattc aaatgataac
    10861 aagtagagaa aatccaattg atatagataa gttattagtt gttacattta ctaatgctgc
    10921 tgcgtcagaa atgagagaga ggatagggga tgctatagga aaagccttag atgaaaaccc
    10981 agaaaataaa catttacaaa accaacttgt tttattaaat aaatctagta ttacaacaat
    11041 acactcattc tgtttggatg ttataaaatc aaactttcat agaataaact tagaccctaa
    11101 tttcagaata ggagaccaaa ctgaatgtgc tattttgaaa caagaagcaa tagaggaagt
    11161 atttgaagat ttatatgaag aaagagatga ggggttttta aatttagttg aaagttatgc
    11221 agaaagagga ggagataaag aagtacaaga tattatcttg ggaatatatt catttgcaat
    11281 ggcttctcct gaacctaaaa aatggttgat tgactcagca gaacgattta atattgatga
    11341 aaattttgat ttttcacagt ctatttgggc tagagctata ttagacactg taaaaataga
    11401 gattaatggt ctttgtctaa atatggaaag ggcattaaaa gaagtggaat caatagaaga
    11461 attagaaact tttgcagaaa agttatctgt tgaatataaa aaaattgctg acatttcaca
    11521 agcatgtaat aaatcttggg atgaggcata taaaaagatg gcaagtatgt cctttgaaaa
    11581 ttatgtaaaa ggagtaaaaa gaatttcaaa agatgctcct tcgtatataa aagaatcaaa
    11641 agaaaaggca aaaactataa gagataagac taagaagtct ctagaaagta tagtaagtgc
    11701 cacattcaat aaagataatg attctattag agaagaaata aaatatcttt ataacatagt
    11761 taaaccaata tctagtgttg tactgagatt tgaagaggaa tatagcaata agaaaagaga
    11821 aaaaggaata atagatttta acgatataga gcattttgca ttaaatatac ttacagatgt
    11881 agatgaaaaa ggtaatattg taccttctga tatagcagtt ggatatagaa acaagtttta
    11941 tgaaatattt atagatgaat atcaggatag taatctagtg caggaagtac tgcttaaagc
    12001 tgttgcaaat acagaaactc caaatagatt tatggttggt gatgtaaaac agagtatata
    12061 tagatttaga caagcaaagc cagaattatt tttgcaaaaa tataataact ataatgataa
    12121 aaagggaagc tcccatagaa aaataatgct ttacaaaaac tttagaagta gagaagaagt
    12181 tgtagatgct gtaaattata tatttgaaaa tataatgaat gagaatatag gagaaattga
    12241 atatacagaa aaagaaagac taaatttagg tgcaaatttt aatgtggata cagacgagaa
    12301 atctattatt ggtggagcaa cggagataca cctaatacaa aaagataata aacttgatga
    12361 tgatattata aatgataaag acgatagaat taataacaaa gaaaatgaaa tagaggaaga
    12421 agaaaaatta gataatatac aacttgaggc tagaatggtt ggaaacatta tcaaagattt
    12481 aatgaaagtc aatgaagatg gaaaaattca aaaggtctat gataagggaa tagatggata
    12541 tagacctgtg gaatttagag atatagttat tcttttaaga gctacatctg catgggctcc
    12601 agtgtttgca gatgaattga tgaatatgga tataccaacg tatgctgatg ttggagtggg
    12661 gtattttgat acgattgaaa taaaaacaat attgtcatta cttcaaataa tagataatcc
    12721 aatgcaagat atacctctta tatctgtatt aaaatcacct atatttggat ttacaccaga
    12781 ggatttgatt gatattagag ttcagagtaa agataaaatt ttttatgagg ttttaaaaag
    12841 tacagcagag tatgatggat ttacagattc tcaaaatgaa aatgagtctg agtttatacc
    12901 aagtgaagag tgcataaaca aaagtaaaga ttttttaatt aagctaaagg aatttaaaga
    12961 aaagtcaatg tacatgagta cagatgaatt tatatggtac ttgtacacaa ggactggata
    13021 ctatgcctat gttggagcat tgccaggtgg aagtcaaaga caagctaatt taaaagtatt
    13081 gtttgagaga gcaaaacaat ttgaagaaac cagtcttaaa ggaatattca actttgtaaa
    13141 ttttatagag aaattaaaaa aatctagttc tgatatggga agtgcaaaaa cacttggtga
    13201 gaatgcaaat gttgttagaa taatgagtat tcataaaagt aaaggtctag aatttccagt
    13261 agtcatatgc tctgcaatgg gtaaaaattt caatactcaa gattttaaaa agagtatttt
    13321 atatcatcat aatttagggt atggacctca atttgtagac tatgagagaa ggatttcttt
    13381 tccaagtata gccaaagaag cattaaaaag caaaataaat atagaaaatt tatcagaaga
    13441 gatgagagtt ttatatgttg catttacaag agctaaagaa aaattgatta taactggttc
    13501 tactagaaat atacaagata gtataaagag atggtcaaat ggaattgaaa gtttagatac
    13561 aatatcacaa tacgagatat taaaaggtaa aaatttttta gattggataa tgccttgtgt
    13621 attaagacat agggatttat ctaatctact agaagaagta ggattagacg cagtttttaa
    13681 tgttgaacat aattcaaaat ggtatggaaa gttgtggaat aagaatgata ttttagtgga
    13741 aaagaagagt gatgaagaaa aagaaagtat tgaagaaatt cttgaaaaaa tagatgtaga
    13801 taatcctgac agtgattatt acagtgaaat agaagaaaaa ttaaactata tatatccata
    13861 tgaatttagt acaagaaaac ctgctactat atctgttaca gaaataaaaa agatacaaaa
    13921 taattatgaa gaagagttaa taaatactat atttgaacaa aaagtaatat taaaaaagcc
    13981 tctatttatt caaaatgaag aagaaagaga aaaaataagt ggaacagaaa gaggtacaat
    14041 agtccattta gttatggagg ttttagactt aaaaaatgta agtagtgtaa atgatataaa
    14101 atcacaaata agaggttttg tttcaaaagg tataataaca gaaaaacaag ctagtatagt
    14161 aaatccatat aaaatatata aattttttgc ctctaacata ggaaaaagaa tgttgaatgc
    14221 tgaaattata aatagagaaa aatcaattta tgcacaagta aatatgaaag acatatatat
    14281 ttatgaaaag cttataaaca atgatgataa aaaactttat gataatgaaa gtgtcatgtt
    14341 aagaggtata gttgatgctt attttgaaga agataatcaa atagttttgg tggattataa
    14401 aacagatttt gtaaatgagg aaaatataaa tcaaataata gagaaatata aaaaacagtt
    14461 ggatttatat gcagatatta ttgaaacttt aactggtaag tcagtaaaag agaaatgtat
    14521 atatttattt ggagttgatg aagctgtttg ttattagttt ttatatataa attaaaccta
    14581 aaagatagag gaaggggaaa aaatgagatt tattcataca tcagattggc atctagggaa
    14641 gtccctagaa ggccattcta gaattgagga acaagcaaag ttttgtgagg aatttataaa
    14701 aatagtagaa tcaaatgaaa tagatatggt tataattgct ggagatgtat atgatacttc
    14761 taatccacca gcacaagcag aaaaactatt ttatcagact gtatcaagac ttgccaataa
    14821 tggtcagagg tgtgtattaa taatttcagg aaatcatgat aatccagaga gactctctgc
    14881 aataacacct ctagcacatg aacaggggat acttatatat ggttatccat taagtgctac
    14941 gatagaagct aagtataaag ggtttgaaat aacatatgca actcagggat gtacaaaact
    15001 gaatataaat ggagaaaata ttgtggttgc tacattacct tatccaagtg aaaaaagatt
    15061 aaatgaagtt ttttcaagtg aagatgagtt tgagaaacaa aaaaattatt ctgaaaaggt
    15121 aggagatata tttagaagtt tagaagaaaa ttttagaagt gatactataa atatagccgt
    15181 atcacatata tttgtaatag gtggagagag tactgaatca gaaagaccta tacaattagg
    15241 tggaagtttt ttagttgaaa gaaaagattt gccagaaaag gctcaatata ctgcactagg
    15301 tcatttacat aaacaacaaa aagcttccga acgtttaaat gcatactatt ctggttctcc
    15361 acttcaatat agcaaggatg aaagagcgta taccaaaggg gcatacatag tggatataaa
    15421 agctggtgaa aaaccaataa ttgaagatgt atattttaat aattacaaac ctatagaagt
    15481 atttaaatgt aatggaatag aagaagcact agatatatgt gagcaaaatc aagatagaga
    15541 gatttggtca tattttgaaa taaatactga tgagataata tcacagaatg aaattaaaaa
    15601 aatgaaggaa ctattaaaag acataataga aataaaacca ataataacat cttgttatga
    15661 acaagaaagt gtagatataa aagaaaagtc tatggcagag ttgtttagag agttctattc
    15721 ttttagtaaa ggtgtagagc caaaaggaga acttatggat ttatttctag atataataag
    15781 tgaagaaggt gaatcagcag atgagaccaa ttagattaga acttacaggg cttaatagct
    15841 atattgataa acaagttata gactttgaaa aattaattga aagaggtttg tttgggatat
    15901 ttggaactac tggaagtgga aaatctacaa tattagatgc aataactatt gctatgtatg
    15961 gaaatatatc cagaaataca aaagaatata taaacagtgt gtgcgataag gcaattatat
    16021 catatgagtt tgagatagga agcaaaaaca ctagaagaag atacattgta gatagaacca
    16081 ttgctagaag caaaacagga acaaagacct ctaatgctag attaatagaa gtattgaatg
    16141 acaataccca aaatgtttta gctgataaag ttgtggaagt aaatgaaaaa gtagcgcagg
    16201 tcgtaggatt aacggcaaat gattttacta ggtcagtagt tttaccacaa gacaaattta
    16261 atgagtttct aagattatca cgtgcagata gaagagacat gttagagaga atttttaatc
    16321 ttgagaaata tggaagaagt cttggagaaa aagtgaaaaa gagaaaaaat atacagttac
    16381 aaaatttaaa agatttgaaa tcaaaattaa gtcaatatga tggaattaca gaggaagtat
    16441 ataacaatat aaaccaagaa ttaatagagt taaaaaattt ggaaaaagat aagaataatg
    16501 ctttagacct agctcaaaaa tcttatgaag aaagtaaaac agtatatgaa gaacaattaa
    16561 aattagaaaa aaatgaactt agaaaaaatg agcttgattt aaaaaatagt gagataaaag
    16621 aaaagattgc tcttgtagaa aatgatagta acgctaaaaa ggtagaccca tacataagtt
    16681 cagttcaaaa tctagaaaaa aagatagatg aagatagctt tgcagtaagt aatttagaaa
    16741 agaaattagc aatattaaat caggagcttg aagttacaaa aaatagatat gaaaaaataa
    16801 acaaaattaa gaatgaagaa gtccctaaat tgagtgaaga aaaaataaga ttgcaacaag
    16861 caataaaatt agaagaagag ttagttttat tagatagaga gttaaaagag ctaaaagaaa
    16921 gtggaattaa tttaaataag actaaagttg aattagaaaa agtaaagcag gtgtcagagt
    16981 ccagaaaaga tgcagttact aaatctataa aagaagttga aggaaagatt gataaagtaa
    17041 atataagtgc agaattaaaa caaaaaatat ttctagcata tgaatacgag aaagattaca
    17101 acaacgtatt ggaagaaaaa aatcaaaagc taaataaact agaagaaata ctcaaagaga
    17161 cagaaaatat aaatttaaaa gttagatata ttgataaaga taagaatgat gtaaatcgta
    17221 atttagaaaa tttatcactt cacttggatg ttcttcttaa aaaatgtcct ggtaagagtg
    17281 ctgatttact tttaaaatct gaatatgtta ctgaattaag aaataaagca aataatacta
    17341 aagaaaatga aattaagaaa agcagtatac aagatgaatt aaagataata cttgaaagta
    17401 agtttaatac tgaaagggaa gtaaatttac taaatgaaaa acttgaaaat aatagaaaga
    17461 acagagatga gctagaaaaa gaattagaag aactaaagta tttaaattta gcctctgaac
    17521 ttagacgtga attaaaagaa aatatgcctt gtccagtttg tgggtcaaaa catcatgaaa
    17581 atcataatat aactaaatat gatgaaaata tatcttttgt caaagaaaaa ctggagaaat
    17641 tagaaaaaga aaaaataagt ataagaaaca atatagagga attaaatgct aaagtaagtg
    17701 ggtatttatc tatagaaaaa atgaagacta aagaacttga agatgtaaaa ggaaaactag
    17761 gggggattcc atctagtcaa ttattaaaaa aacttgatga agaacaaaga aaacttgcat
    17821 tgttaaaatc taatattcag gagtgggaga aagaaaaaga gagtactgaa aataaagtaa
    17881 cattagctaa agaagaaaaa aataaaattg aaaaagaaga attaaaaata agagagagtc
    17941 tgaataatta taagaaatta acaagagatt taaacataga aatagaaggt ttagaaaata
    18001 aatgtaaaaa acttaaacag gaatacctag gattaaagac gattactaaa gtctctaatc
    18061 tattgtcaaa agtaaaagaa ataagagaga atgaaaaaga attagaatta ctaagtactg
    18121 ggcattcaaa tttattaaaa aatagagact cactagatat taaaataaga gaaaatgaga
    18181 gtaaactaca tgaaatagag atagaattaa taaaggctag agaattatat gtagaaaaga
    18241 aattgagtag ggataataag tataaagaag ttattagcat aacgaaaggt gatttagcta
    18301 aaaatctttt acataatgtt gaagaaaata tatgtaagat attagaacaa gaagaatcaa
    18361 gtaagaaaaa attggaagag caaagattag aatatgaaaa aaacattgct gaaaagcaca
    18421 atatagatgg tagacttaaa actgctaagg aacaatataa agaccaaaaa tcaacactaa
    18481 ataaactatt ggcagaaaat aaatttgaaa gtatatatgc agtaaaaaga gctttattag
    18541 atactgatgc agtaaaaaaa ttgattgaag aaatactaga gcatgaagaa gaacaaaagt
    18601 tattatcatt taaaataaaa tcatcaaaag aaaaattaaa tggaagaagc ataaagaagg
    18661 agtactttga acagttaaaa gatgaaattt ataatttgaa agtagaaata ggaaatatat
    18721 ccaaagatat tggagctaat caaaatcagt taataacatt aaaagactct ttagataaga
    18781 taaatgattt taataaacaa cttaaggtag ttgaacacaa tgtggactta ttggaagaat
    18841 tagataaatg tgtacaagga aataaatttg tagaatatgt ttctacaaac caattaaaat
    18901 atatagcgtt ggaagcatca aaacgattgg atggaataac aaaaggaaga tatgcacttg
    18961 aaattgactc aacactaaac tttgtaatga gagataactt taatggagga gaaagaagaa
    19021 gcattgatac tctatctggt ggagagacat tcttgacatc tctatcatta gcacttgctc
    19081 tttcatctca aatacaatta aagggaagtg caccattaga atttttcttt ttagatgaag
    19141 gttttggttc attagatagt gagcttttag aaattgttat agaatcattg gaaagattgc
    19201 atagtaataa tctaagtgtg ggtataataa gtcatgtcga ggaacttaaa aatagagttc
    19261 ctgttaagct attagtttca tctagtgagg ctggaatagg ttcaaaagta aaaattgaat
    19321 atagttagaa aatatatgtt aattgaatat attatctaat atttccaaat aatatgtaat
    19381 tgtgatataa taaaaactaa tattaaagtt atttataatt aatattaata taaaaacatg
    19441 taagtaggag tgtggtaagt gttggaatcg cccaataatt tgattcagat tattttttta
    19501 atagtattac ttatagggtc tgcatttttc tcagcatctg aaacggcctt aatgtcgttg
    19561 agtaaaatca gaatcagata tatgcaagat gaaggagtta aaggagctaa gttagtaagt
    19621 tcattaatag aaaatccaaa taagctttta agttctatat tggttggaaa taacgtcgta
    19681 aatatagcag caacatccat atcaacatct ttgtttatag gattaatggg agaaaaagga
    19741 gtggcacttg ctacagctgt tatgacagta ttagtattaa tatttggtga gattactcca
    19801 aaaacaatag cagcaaataa ttcagaaaag gtttctctat tagtatctaa acctataaaa
    19861 gcaattatat ttatattaag accaatagta tggatattta atataattac aaatattata
    19921 tttaaattat ttggtataac aaataaagga gctaaatcat tcataactga agaagagcta
    19981 aaaactatgg taaatgtaag tcatgaagaa ggcgtccttg aaatggaaga gagagaaatc
    20041 ataaataatg tttttgaatt tggagatatg caagctaaaa atgcaatggt acaaagaata
    20101 gatatggtag ctatagatat ggaagatagt tatgatgaga taatacaggt atttaaaact
    20161 gaaaaattaa gtagaatgcc tgtgtatgaa gaaactatag atgatattgt aggaatactt
    20221 aatataaaag atattatatt tttatctgat gaagaaatag agtcatttga tattaaaaat
    20281 tatatgagag aacctttttt cacgtatgag ttcaaaaaga taacacaact tcttgaagag
    20341 atgaaacttg aaaaaagtca gatggctata gttgtagatg agtatggtgg aacatctgga
    20401 cttttaacta tagaagattt agtagaagtc attgttggag atatagagga tgaatatgat
    20461 gaagaagagg atgaaataca agttataaaa gaagatgagt atattgtaga tggaagtact
    20521 aaaattggtg atgttaatga acttattggt gtaaacttgg aatctgagga gtttgattcc
    20581 ataggaggtt ttataattgg acacctaagc agattgccag aagaaaatga agtaattgaa
    20641 gttgataata taagattttg tatagaaagt atagaaaaaa atagaattaa aaaaataaga
    20701 atatatacat aatgtaataa tgttcttaaa tttat
//
LOCUS       DACXMZ010000048        20707 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.048, whole genome shotgun sequence.
ACCESSION   DACXMZ010000048 DACXMZ010000000
VERSION     DACXMZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20707
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.048"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="JD016_06925"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="JD016_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAU4999370.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /locus_tag="JD016_06930"
     CDS             773..1402
                     /locus_tag="JD016_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAU4999371.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="JD016_06935"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="JD016_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAU4999372.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /locus_tag="JD016_06940"
     CDS             2055..2345
                     /locus_tag="JD016_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAU4999373.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="JD016_06945"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="JD016_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAU4999374.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="JD016_06950"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="JD016_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAU4999375.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /locus_tag="JD016_06955"
     CDS             3553..3888
                     /locus_tag="JD016_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAU4999376.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="JD016_06960"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="JD016_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAU4999377.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="JD016_06965"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="JD016_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAU4999378.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /locus_tag="JD016_06970"
     CDS             5195..5398
                     /locus_tag="JD016_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAU4999379.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="JD016_06975"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="JD016_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAU4999380.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="JD016_06980"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="JD016_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAU4999381.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /locus_tag="JD016_06985"
     CDS             6094..6399
                     /locus_tag="JD016_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAU4999382.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="JD016_06990"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="JD016_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAU4999383.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="JD016_06995"
     CDS             6989..7174
                     /locus_tag="JD016_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAU4999384.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="JD016_07000"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="JD016_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAU4999385.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /locus_tag="JD016_07005"
     CDS             7636..8178
                     /locus_tag="JD016_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAU4999386.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /locus_tag="JD016_07010"
     CDS             8214..8582
                     /locus_tag="JD016_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAU4999387.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /locus_tag="JD016_07015"
     CDS             8603..9112
                     /locus_tag="JD016_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAU4999388.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="JD016_07020"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="JD016_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAU4999389.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /locus_tag="JD016_07025"
     CDS             9345..9788
                     /locus_tag="JD016_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAU4999390.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="JD016_07030"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="JD016_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAU4999391.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="JD016_07035"
     CDS             11117..11767
                     /locus_tag="JD016_07035"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAU4999392.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="JD016_07040"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="JD016_07040"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAU4999393.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="JD016_07045"
     CDS             12536..12805
                     /locus_tag="JD016_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HAU4999394.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="JD016_07050"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="JD016_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAU4999395.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="JD016_07055"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="JD016_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAU4999396.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="JD016_07060"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="JD016_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAU4999397.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="JD016_07065"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="JD016_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAU4999398.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="JD016_07070"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="JD016_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAU4999399.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="JD016_07075"
     CDS             14901..15848
                     /locus_tag="JD016_07075"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAU4999400.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /locus_tag="JD016_07080"
     CDS             15869..16210
                     /locus_tag="JD016_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAU4999401.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="JD016_07085"
     CDS             16330..17163
                     /locus_tag="JD016_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HAU4999402.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="JD016_07090"
     CDS             17151..18017
                     /locus_tag="JD016_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HAU4999403.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="JD016_07095"
     CDS             18011..18814
                     /locus_tag="JD016_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAU4999404.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="JD016_07100"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="JD016_07100"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAU4999405.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="JD016_07105"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="JD016_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAU4999406.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="JD016_07110"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="JD016_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAU4999407.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8265 a         2447 c         4323 g         5672 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaaagagggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt gtcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgagaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctacttcat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc taggtgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tattccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaagtgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtactaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tacagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattt
//
LOCUS       DACXMZ010000049        20559 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.049, whole genome shotgun sequence.
ACCESSION   DACXMZ010000049 DACXMZ010000000
VERSION     DACXMZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.049"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..518)
                     /locus_tag="JD016_07115"
     CDS             complement(<1..518)
                     /locus_tag="JD016_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAU4999408.1"
                     /translation="MIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDYILKL
                     GDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIKSKVT
                     DNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGILTNP
                     NLYYQMTFTFNPV"
     gene            complement(508..1200)
                     /locus_tag="JD016_07120"
     CDS             complement(508..1200)
                     /locus_tag="JD016_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU4999409.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKGKEEMVKELKKYESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
     gene            1545..1745
                     /locus_tag="JD016_07125"
     CDS             1545..1745
                     /locus_tag="JD016_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999410.1"
                     /translation="MLYVVNNNVIMQTPKATNSPDKVSTSLFKEITTSLLIDELKNMY
                     EIIHINTGVKTNSTTSLEFIAL"
     gene            complement(1801..2775)
                     /locus_tag="JD016_07130"
     CDS             complement(1801..2775)
                     /locus_tag="JD016_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999411.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFDKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            complement(3078..3314)
                     /locus_tag="JD016_07135"
     CDS             complement(3078..3314)
                     /locus_tag="JD016_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999412.1"
                     /translation="MPNLGTTFVTEEAVYNMSFRSNKAEAKLFTKWVTKALKQIRIHG
                     YYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVY"
     gene            complement(3799..4278)
                     /locus_tag="JD016_07140"
     CDS             complement(3799..4278)
                     /locus_tag="JD016_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999413.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMIKELGVYLFGIDGLKLTV"
     gene            complement(4432..5802)
                     /locus_tag="JD016_07145"
     CDS             complement(4432..5802)
                     /locus_tag="JD016_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAU4999414.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCALTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKDGAENA
                     IHNKIKDICISLKAEDYLNMPKKIDNKIEIHLDSKILKYYKELEKEKILELEKDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKDTIDEQVMEALENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            complement(5751..6077)
                     /locus_tag="JD016_07150"
     CDS             complement(5751..6077)
                     /locus_tag="JD016_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HAU4999415.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            complement(6359..8776)
                     /locus_tag="JD016_07155"
     CDS             complement(6359..8776)
                     /locus_tag="JD016_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999416.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIETFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDAGVKKTNKSIFNGQLDNERNQIWAEAVELYKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITKNWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            complement(8788..10746)
                     /locus_tag="JD016_07160"
     CDS             complement(8788..10746)
                     /locus_tag="JD016_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999417.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTSISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWLAGEKWRLDVFNSHGKIYEASASQMFKIPIENIKKGSELRQKGKLAELSN
                     GYGGSVGALISMGAIKMGLKEEELQPIVTAWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VEIQHGIKFIYNPGVLFIELPSSRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            complement(10746..11318)
                     /locus_tag="JD016_07165"
     CDS             complement(10746..11318)
                     /locus_tag="JD016_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HAU4999418.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            complement(11341..11913)
                     /locus_tag="JD016_07170"
     CDS             complement(11341..11913)
                     /locus_tag="JD016_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HAU4999419.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            complement(11966..12301)
                     /locus_tag="JD016_07175"
     CDS             complement(11966..12301)
                     /locus_tag="JD016_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999420.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     ILNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            complement(12316..13482)
                     /locus_tag="JD016_07180"
     CDS             complement(12316..13482)
                     /locus_tag="JD016_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HAU4999421.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPSTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            complement(13494..13931)
                     /locus_tag="JD016_07185"
     CDS             complement(13494..13931)
                     /locus_tag="JD016_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999422.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            complement(14132..14524)
                     /locus_tag="JD016_07190"
     CDS             complement(14132..14524)
                     /locus_tag="JD016_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU4999423.1"
                     /translation="MDISESITKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVERGDEIAES"
     gene            complement(14555..14752)
                     /locus_tag="JD016_07195"
     CDS             complement(14555..14752)
                     /locus_tag="JD016_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999424.1"
                     /translation="MLIGDNIKQILRKRDIKPYKLAKELDIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            14828..15202
                     /locus_tag="JD016_07200"
     CDS             14828..15202
                     /locus_tag="JD016_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999425.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            complement(15212..15421)
                     /locus_tag="JD016_07205"
     CDS             complement(15212..15421)
                     /locus_tag="JD016_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999426.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(15468..16298)
                     /locus_tag="JD016_07210"
     CDS             complement(15468..16298)
                     /locus_tag="JD016_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HAU4999427.1"
                     /translation="MSNNLMVFEGKEVEVFEFEGQILFNPKHVAECLNIKNVNENLRN
                     MNNKQVIKLINSKISSTDFRKLHNTGENFLTESGVYKLIFKSRKEEAERFQDWVTDEV
                     LPSIRKTGTYNMVNRQLKDSYMIDNPIERAKRWIEEQKEKEQLQLEGKMKDQVIKELK
                     PKADYTDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGK
                     GYTHSETIDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKEYSYQTSILG
                     "
     gene            complement(16418..17191)
                     /locus_tag="JD016_07215"
     CDS             complement(16418..17191)
                     /locus_tag="JD016_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAU4999428.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKRKKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFDEMEKALKNEQTKLPTTYKEALQQLLIEVEEKEQLQLENQTMKPKADYFDALVER
                     NLLTNIRDTSKELGVKEKVFVLWLIEKKYCYRDLKGKIKPYSNKMQYFELKEFTTPYG
                     HSDTQTLINPKGREAFRLLLIKDGLIREKERECQITLLG"
     gene            complement(17222..17437)
                     /locus_tag="JD016_07220"
     CDS             complement(17222..17437)
                     /locus_tag="JD016_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999429.1"
                     /translation="MYVNRLKGLMKENQHTQKFVADLLGLSLFGFRLKLNGKNEFKAN
                     EIKKLSELYGVSTDYFFSELVAKMAIK"
     gene            17618..18163
                     /locus_tag="JD016_07225"
     CDS             17618..18163
                     /locus_tag="JD016_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU4999430.1"
                     /translation="MFRLKELREEKGISLDRLSMDLNVNKSTLSRIENGLREPKQSFI
                     EDCSNYFDVSTDYLLGKTDIKNPNQVVKSLNPIFSKRLRELIKEKGVTLNILGKKLEI
                     STDLLSKYENNIISPKSDDIIKLAHYFDVTTDYLLGTTLVRNHIDTVAAHKANPHEDL
                     PEEAQEQLNDYIEFLMNKYKK"
     gene            18187..19776
                     /locus_tag="JD016_07230"
     CDS             18187..19776
                     /locus_tag="JD016_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999431.1"
                     /translation="MKSIISDVINRFNATPFLFVGSGLTRRYYNLPNWEDLLKVFAEK
                     ISNDDFIYTSYKNKAKSENPKMGINPRIAELIENDFNKKWFSDSSIRSLDSKYLDIVK
                     SGVSPFKAEIAMYIKNNSKIVDKYQDEVEKLSNISKKSLSGFITTNYDCFLETIVDNY
                     TTYIGQENLVFSSIQGIAEIYKIHGCVSSPNSIVINEADYIDFDSKSAYLAAKLMTIF
                     VEFPIIFIGYSVTDVNIKKILNAIVNCLSNENAKKLEERFIFIEYEKNFNDIEISSHT
                     IAFDNKMITMTKIKLEDFNLLYEALSEKKSKLPVRILRMFKQEFYDFTITNKPTAKIR
                     VGSVDDTRIKDEDLVLAVGKASDFGLKGLKGLSFDEWYRDIVMNDLEFSSDELLEYAY
                     PSLIKQYNKLPLNKHLFNSTLEFPDYRTIALESDFENIISNSIKKNRNNTYIKNRSVY
                     GIWNDEHQSFEKATRLIAFLEEKEIDTEQLENLLKKIFEENPNILESAKTSEKTNLRR
                     LIRIYDYMKYSNMQKSLGYSE"
     gene            20039..20545
                     /locus_tag="JD016_07235"
     CDS             20039..20545
                     /locus_tag="JD016_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAU4999432.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
BASE COUNT         6364 a         3721 c         2149 g         8325 t
ORIGIN      
        1 actggattaa atgtaaatgt catttgatag tataggttag gattagttaa aatacctctt
       61 aaacggtcat ctagtatgtc tatgtcactt tccataagtt ctgtagcttc ttcacaccaa
      121 acccatgtta attttccttt cgagaagtta attgatttta atttttctct ttgttttgca
      181 tcattaactc ctctgaaaat tatagagtta tcagtaactt tactcttaat ttctaaagga
      241 tttaaagtag ttttccaata cttatcagct tgtttaccat aaatacgatt tatagctcct
      301 gtaagctctg catacgttga atacttatgt gtagcttctg actttctaac tactaataga
      361 ttagctcctt gatacttctt atctcctaac tttagtatat agtcttgtgc tacattaaca
      421 gattttccac tccctgctga acctttcatt gctctgtatc tttttttagt aaaattagct
      481 tccttgaaat ctggattaaa atttactcta actatcattt ctatcaccat aatctacact
      541 tattttcaac tcatcatctc caatatcatc tttacttagg ttatcaactt cacatttcaa
      601 cttctcaact cttgttttct gctcctctgt agccaaattc caatccttat gaatcatttc
      661 atcatactgt ttaattaaac ttctaagttc actcatagct ctactctgtg cattaagaaa
      721 agatgcttgc ctatcccatg caaattgaaa ttcatactct atcttctcac cattttctgt
      781 gctttcatat ttctttaatt ctttaaccat ttcttccttg cctttaacat acattatctt
      841 ctgtgctctt attattgctg cgtattgaat tgttatctgt tcccaaagaa tatcaaattt
      901 atcttttata gatatttctt gtatcaattc cctagtttct tcgggtagat attttgagaa
      961 gaaaccaaac ttttcagcgt tcttattctc ttttggagca ccatgaccaa ctgaattttt
     1021 attagaaaag ggtgcacctc ttttatttat aggtgcaccc ttcttttttt cactagccca
     1081 gttgtatctt tttatccatg actttaaagt atttaagcta atgtcatact ttgatgatat
     1141 ttccttttgt ttcattcctt ttaagtaatc ttgttttacc ttttctttga catcttgcac
     1201 atcaccacct ctttatttgt ttgttttggg aataaaaaaa acaatcctat ttaataggat
     1261 tgtttcaaat ctagtctcct ctatccttcc aggataaatg aaacatactt attattaatg
     1321 tagttagaga taaaattata tgaactgtaa aaaatattaa aacatagaaa attatattag
     1381 aataatatcg agtaagcatt attgtattga taaaacatat tacaacaacc aaaatctcca
     1441 ttattacagt ataatatgct tttaactgta tgaattgaaa gtatgatata ttaaatccat
     1501 ttacctttct ttctgttttt atatcttttg cttctgatat tgatttgtta tatgtagtaa
     1561 acaataatgt aataatgcaa actccaaaag ctactaatag tcctgacaaa gttagtacat
     1621 ctttgtttaa agaaattaca acttctttat taattgacga attaaaaaac atgtatgaaa
     1681 taatacatat taatactggt gttaaaacta actctactac ttctttagaa tttatagcct
     1741 tgtaataatt gagtaagata tttaatacct ttttatacat tttaacacct ccttcgcaat
     1801 ctatgcttca ctcatagcaa cttcttgata cattaaatta aaaaaaactg aattattctc
     1861 aattaaatca tctatcaaat tattcatctt atcaaaaatg tcttcactat ctatatgatt
     1921 atctatatca agagaaacct ctatttctct acacaacttt ataccctcag tatccaactt
     1981 taattttctc ttgtttttct ctccctcaat tattatccta tttatttttt tatttctaaa
     2041 ttcctctcct ttaatatatt tttcgcaaca tcttttaact tcactaggta aatatctagt
     2101 tctaaatgtg ggcttatata tcaaatcgtt atgtcgtctt gaattattta aattagaaaa
     2161 caaattatct tcatcggttc caaacttctc tctatcaaca tttaaagtta tttttgaaac
     2221 accatctaaa tctaatattt gttctataaa atctttactt gctatttgtg taatattaat
     2281 tgatgttttt gacatattat tatcaaattt attattttca atcatatcct cataatatag
     2341 atgtaagttt ttacttagtt caatcatggg aacccccaca aaaactcttt caaataatac
     2401 tactgctata ttattttcct tcataaattt aataaatagg tgctgcttat tgctatctcc
     2461 ttgatccatt tttttattac cctctacttc tttagtatga gcatttataa tatttgtatt
     2521 ctgattgtat ttagtatatt caagtttcac cttgattatt aaatcatcct cgctacattt
     2581 tacttctgta atccaaaact ttttatcttt agtaatatta attccatttt tgttttcttt
     2641 acaatattct ttatccttaa agaaggtctt tattacctct tttaaaattt cttcatctga
     2701 atagacactc ccattaattt tatttataat atctattcta taaacatcaa tatatctttt
     2761 tttaaaatta ctcattatac cattattcct ttcacaaata tttataatga tataattcta
     2821 tataatcagt caatatcctt caataatcat tcgacaacca caaaataagt ctaaataata
     2881 cttttacctt attcctgtct ataatgtaca aatatatttc atttttaatt cattacataa
     2941 aaaaaagacc ctctattaat acagtcattt aaatcatttc tattaattct ttaatctttt
     3001 atatacctct ttataattca tacttttatc tatcaaccta ggtgatttca tagatataac
     3061 tctttcaagt gcttgtatta ataaacaaac tcttgtattt catctgtaaa atcttttctg
     3121 acctttttac cttctgttct tatatccagc cattcttgat ctttttctgt ggcaatatag
     3181 taaccatgta ttctaatttg tttaagtgct tttgtaaccc acttcgtaaa tagttttgct
     3241 tctgctttat tacttctaaa tgacatattg tacacagctt cttctgtaac aaaagtagta
     3301 ccaaggttag gcaatttatc tttaaagttt ctagtgtagg aatctccgac agtagactca
     3361 ttaatttctt tttatattct ctatctatat ttcttaatgt atcacgaata tttactatgc
     3421 ctagttcctc tcctacgtca tttgcattaa atcaaacttc ttctccattt tttgaccata
     3481 tcatccttac atttttctct tgtaaaattt tcaacatact actacctcct aaattagtta
     3541 ttaataaaga atattcaatt tgaacaccct tattttcgac ccttcaatat gagggattaa
     3601 aattgtataa aaaagaccta gaaatcactc taagtcatga attactcatt tatcattctc
     3661 cttatttaca aaataaaaaa gactaagttg gggtaactta gcctttttca agggggaata
     3721 tattatacac ttgtttcata ctaccattat aacagggtta aaatatcatt aaaatatcat
     3781 ctttttatca tatatttatc aaacagttaa tttcaagcca tctataccga ataaatacac
     3841 tcccagttcc tttatcattt cattaatcca acgtcttgat gtaactactc cacaatttaa
     3901 tatctctgct atttcttcat atgttttttc atcacaatag taaagctcta aagctctata
     3961 cttttcataa gaatgtaaac gctcttgtct taatttcaaa gttttcattg ctgaatctat
     4021 atgtgctatc ataataatag ttcttgcttt acttttcttt atacttaaaa tatacaactc
     4081 ttcattatca agcttaccta aatcatcctc taagtaatct acatctttag cttcacttac
     4141 cgctgagtct atatgtctct taaaatcatt ataatgcttc atcaataatc tagtattata
     4201 taaaacgcct tttttcctat cttctctttc ttcttttctt aactctctta caatttcttt
     4261 tatactttct ttatccactt aaatcactcc ttcttgctct cttcgaacaa cttctactgc
     4321 aatacttaac tttagattta tccaacttat aaaatcttct accacaccaa gcacatctta
     4381 ctattttacc tgggcttata agttccatct tttcttttta ctttcatacc ttcactccct
     4441 atattctttt aattttgctt tgactgcttc aagtaatgca ctttgtcctt tatctttatt
     4501 ctctagagcc tccattactt gttcatctat tgtatcttta cagattagat gatggataat
     4561 aacagtttcc ctttgcccct gtctataaag ccttgcattg gcttgttgat acagttctaa
     4621 gctccaagtt aatccaaacc aaacaattat acttccacct gattgtaaat taagtccatg
     4681 tcctgtactt gctggatgac aaagtaataa ttgtattctt ccattattcc aatcatatat
     4741 atcttttgag ttttctattg ttcttggttt caaactctta aactctttca ttaaacgatt
     4801 ataatcatgt ttatagttat aaaaaactat aataggctta ccatttgaaa catctataat
     4861 ttcttttaaa gcttctaatt tttctctatg gagttccttt acatttttat cattatcata
     4921 aattgctcca ttagctaatt gtaaaagttt atttgctgct acagctgctg atgaagctgt
     4981 tattatatct ttttctaatt ctaatatctt ctctttttct aattctttgt aatatttaag
     5041 tattttacta tcaaggtgta tttcaatttt attatctatt tttttaggca tattcaaata
     5101 atcttctgct tttaagctta tacaaatatc ttttatttta ttatgtattg cattttctgc
     5161 tccatcttta ggctgccaat tatatatagc tcctgtttga taattttttt gtcctggctc
     5221 aaagtatcgt tctttatatc ctgtaatagt tttacctagt ctttcacctc tatcaagtaa
     5281 gtacatctgt gcccataagt ctattaaact atttggtgtt ggtgtaccag taagcccaac
     5341 tactctttta gttaaaggca atactttctt taaactttta aatcgttgtg ctttatttga
     5401 tttaaaagaa ctaagttcat ctatcacaac catatcaaat ggccattttc ttttataaaa
     5461 atccactatc cagggtacca tttctctatt tattatataa atatctgagt ctgtacttaa
     5521 agctctcact ctatctaatt tactaccaag aactttagat acttttagat gctttagatg
     5581 ctcccatttt tttacttcac tactccaggt atctcttgca actcttaaag gtgctataac
     5641 taaaactttt gatatatcaa agtaatcata cattaattca cttatagctg ttagagcaca
     5701 gactgtttta cctaatccca tatctaatag taaacctata ttattattat ctatagtttt
     5761 tctaattgtg tattcttggt atggatgtgg tttaaattcc atcccttaac ctccttgata
     5821 aaatcatcta tctcttttaa tgtgcttaca cacttaactt taaatcctaa ttctcttaat
     5881 tctcgcattt tatattgttg aagctttctt agttttttac ctggtgcttt aagttctaca
     5941 aatataacat gtccttctgg taataagaca atcctatctg gcacacctgc ctcacctggt
     6001 gaaataaact tcatagcctt tccacctaac aactcaatct cttttttaag tcttttttct
     6061 atttttgatt ctaacaaaat atcacttcct taaaatttaa gtgtcaacaa tgtcaacaga
     6121 ttttctatat atatgtatat atacatatta ggcatacata tgtatatacg tatatgccta
     6181 atattactta tactacttta tacttaattt ttgttgacat tgttgacatt aatacctcaa
     6241 acattgtaat tactgcatct taggtgtcaa cataagcgtc aacaaagcca ttttttttgt
     6301 tgacattgtt gacactttta aaattttcaa atttactcta tttttaattc tgttgacact
     6361 aacttttacg tacataagct ctctgcctac catatatttt cccaaacctt aaatttccat
     6421 tatatgatat ccaatcatct aatcctttta atatatcatt aatttctctt gataagatag
     6481 gcgtgagttg tttaggttct ccattaaata gctcaaccca tatctccata acacacgttt
     6541 tttctcttaa tattgtccct tcttttaaat caccaaaatc tgaaccatga atatattctc
     6601 tcttttctga aatacttaaa tcataccaat tcttagtaat aggtttattt agatactctt
     6661 cgataattcc agatttagca ttttcttctg agtgagttct ttgttgtctt tcagcttctt
     6721 ttttctcctc atctgataga tatagctgtt cattggcttt atataattct actgcttctg
     6781 cccaaatttg atttctttca ttatcaagtt ggccattaaa tatactcttg ttagtcttct
     6841 ttactcctgc atctattggc caaaaccttc tattcccagt cttatctctt aaaaattctt
     6901 tatcattagt tgttcctata actacacatt gacgtaaaaa tcttgaggtt ctcttcccat
     6961 atgcgaccct atatatatcc tctgatttgc ttagaaaatg cttaactgcc tcaatatctg
     7021 cttttttagt agccatcatt tcacccattt ccagcaacca tactccttgt aattgttcat
     7081 acgcttcttt accttgtaca gtagttaaac tatcagaata ccagtctcca ccaagttttt
     7141 taataaaagt actttttccc attccttgag gaccagataa aaccatcata ttatcaaact
     7201 ttattcctgg attaaatact cttgctactg ctgctactaa aacttttctt attatagtcc
     7261 ttgtatagtg gttatcttct gcacctaaat agtcaattaa aagtgtatcc actctcttaa
     7321 tgccatccca ctttaaagaa tttaaataat cttttatagg atgaaaagta ttattttcaa
     7381 aagcaattat tagagcatca tttacttttg atggtgatga gatattgtaa attgtttcta
     7441 tatgatgtct aagcccagaa tcatcactat cattccaatc atttaattta tcatcttttc
     7501 tccaaggtaa cttacctaaa acgacagctc tatttgaaaa ttcattataa gctatttttc
     7561 ctttcaaata tgggtcattt tctataaaca ttaagatatt gtttgttgtt ttcttataac
     7621 ttcccttatt gtcataatct aacctagtta accattcatc atcttcaaaa tctatatcac
     7681 caaaatcatc cttagcttta tcaaggtttt ctcttcctat agtctttcgt actttagtgt
     7741 cactgcttgc aaattcgctc attcgagtaa atgaaggtaa tctatttaca ggcgtttcgg
     7801 gtttagcatc ttcatcaagt tcaccaaatt tatgtatcct aactaaatca aaagcattgc
     7861 ataaaattcc gcttgctggg tctgtaccat gatgactata tgaaaacttg tcatcataaa
     7921 taactactcc accacttgta ctaccttctg aatatgtata cctggtttca tcaataccag
     7981 gaatatatac ttcatttaag aaagtttcta tagtttcttt tatactatag gacctgcaaa
     8041 atgcacctat aattccagcc ttttcaatag ggtcttgttg tttttttaat tgtgtattaa
     8101 acttttgcct ctccctcgaa ctttctggcc aatagcttac atctgtccag tctaaatata
     8161 aatctagtat ttcatttggg tttaaaaact ctccatcttg aaatttaaat atatagtctc
     8221 catcaattga agtacttgga aaatacataa gtctatgagg ttgatatgta gtatcatcaa
     8281 acatatctat tcctatagta tctgctatca tcctggaaat tgcttgatat tcttctggta
     8341 gtactggtct agttaaaggt attacaagtc tatatctggg attattctca gtatgtgagt
     8401 gcgtagaata cataagacaa gcataatcat ttaataatgt tatatcctcc catatatctt
     8461 tattcgcata atctatatct aaagttataa tacttctatt tgcaatattc tctgcttttc
     8521 gtcttccatt ttttaagctt ccacctacaa atccaccaac atctttaact ctatcttttt
     8581 cagtctttga catcttccta tattctgtat atgtttcttg agttcttaat gttttactta
     8641 atctatttac aagttcggac cataaaatac ttttattttt ccaatgtgtt tctaatttat
     8701 tttttcctat ggccagcatg agttggccat catgtcttac atttatgtgt tcaatttcac
     8761 tggcctttat atccataaat caatcaccta gtctttctta taataatcac attcatatcc
     8821 atctgcttta agaggaagac ctttagccca agatatttct tttcccataa tactgttaac
     8881 ttcttctaaa gaacctcctt ttttatctac atcaattaca agctcatcat gtacatgcat
     8941 tacaatgcta taacctgcat ctgtcacatt aaacatagcc tctcttaagc aatctctagc
     9001 tgtagcttga acaatattct caactaactt aggtccataa gtatctattc ttttccattg
     9061 tttacttgtc tgttccatac cttcatatgt tatcttatcc ccactaaatg tagtatgtgg
     9121 ctctatctta ggccttagat acgataatct tctactactt ggtagttcta taaataaaac
     9181 gcctggatta taaataaatt taatcccatg ttgaatttct actatagtcc tatcttttat
     9241 agctttttta gctgccttat ctacatccca ccaaaactta gttatatttg gattagcatt
     9301 tctccatgca gtaacaatag gttgaagctc ttcttcttta agccccattt taatagcccc
     9361 catagaaatt aaggccccta cacttccgcc atatccattc gataattctg ctagctttcc
     9421 tttttgtcta agttcagaac cttttttaat attttcgatt ggaattttaa acatctgact
     9481 ggcactagct tcatatattt ttccatgaga attaaacaca tccagtctcc acttctcacc
     9541 tgcaagccaa gctataactc tagcttctat tgcactaaaa tctgacacta taaatctatg
     9601 accttcactt ggtataaaag ctgtccttat caattgactt aagacatctg gtacactatc
     9661 atataaaagc tctattaaat caaaatctcc ttcttttaat aggtttctag ctaggtctaa
     9721 atcctctata tgattttgtg gtaaattctg tacttgtact aatcttcctg cccatctacc
     9781 agtcctatta gccccataaa actgtagtag acctcttact ctattgtcat tacctttagc
     9841 taatttcata gcctcatatt tctttataga agttttggac attaattttc taagttctaa
     9901 aattctaact acattttcat catcaacttg ttttaatatt tctggaatac tttcttttgt
     9961 taggcttgta atctcaaagc caactttatc acttaaccat ttttttaatt gagctggact
    10021 atttggatta tttagaccag ttattttaat tgcttctttt gtaagttttt cagtatatct
    10081 tttatcacat tctattgcat tctctattaa ctctgtatcc actttaatac cagtatcatt
    10141 aattctctgg tctaaatacc ataatttaat ttctctttcg gtagtcttat acttactaag
    10201 tttgtttctt atttctcttt caactacaac atcttgttta caatattctt taaatttatt
    10261 ccatttttcc atatcatgta ttggtaaatt tctagttctt cccttgttaa cttttgtagc
    10321 cttacaaggt ttacagaaat attgtattaa tgctttacct tctttcattt tctgtttatc
    10381 ttcattaaac tttaaagcct tagacacact atctaaactt cctggaagtc ctagtgttaa
    10441 cgcctttatc attgtacatg accactcatt tggttttaaa ttaatattta aaaatttact
    10501 tattgatgtt ctttcaaaat tagcattaaa tgctgatttt ataactttat tatcatttaa
    10561 agcttctatt acttcttttg gtaactcttc atcatttact aaatcaataa cttttacctc
    10621 ttcattatca aaagcatagg caaataacag tatctcaaaa ttagcagagt ctacatatct
    10681 gtagactcca acttttttta tatctaaatc actatatgtt tctatatcaa ttgataaggt
    10741 cctcattaac ttaagaagtc ctcttcttca tcttcatatt caaagtcatc tgagaaatct
    10801 gcttctgcac tagctctagc tcctccaagt acttctccat ctgctaactt ttgaacattt
    10861 tgtaagccac aacctattcc tttatttcct gcactattat atgggaaaaa gtttatacta
    10921 actcttccat aacaaccact atatacctct gtattatcta atatttcatt taagtcttta
    10981 tcaactattc ctggcttttg agtactattt gcatttaaaa aatacattcc tacatattcc
    11041 tctgcttcat cagctctttc tgcatctcca tcacgaagag gtgtttttaa attacctggt
    11101 aattttccac cccatttaga agttttacct tgttctttag ctgcatcaat agccttcttt
    11161 attcttccta aagtcacctt atctgatttt ggtattaaaa tacaaactga atacttgggc
    11221 tctgcacctt ctaccattgc tctgctttta aagatattgc aataacttaa tcttaccttt
    11281 cctgttacta cctttgttga ttgtactgaa ttactcataa ttttcttcct cctaatattt
    11341 ttatatttgg tatattgatt ttatatttga agttatttct ccaaatttat ctaaatctct
    11401 aacttgctta ctaaacaatt taattcttcc acaaataact gttgaagaaa catttaataa
    11461 ctctcctata tcagatatct ttttatcttg taaaatccaa tctactattt tatcaatatc
    11521 ttttaaatat gactttcttg caatttttct tatataatca acatcatagt taaattgaat
    11581 ttcataaaat ccatgtatat cttttaatgg tcttttacta tcttcaaata cacttacatc
    11641 taaaccttct atagagtttc tataatgttt ttcgccataa cgcaaagctt taaacatttc
    11701 tattcttatg taagaaacag ccactgtaga aaacttgcct ctgttactat cataatttaa
    11761 tgctgcttta tataacccaa tacatccttc ttgataaaac tcttcatata agtatggatg
    11821 ttgctctaca taagacttaa aaaatctatt tatactaaaa tgtacaagat tcaaattatt
    11881 ttcaactagt ttagttattt cttgattact cataaaatta tatccccttt taaatctttt
    11941 tatagctact aggaattaaa tatttttaat tatcctttgc tcttactgat acaaagcata
    12001 ctggactaac ttttatatgt tctggtatat acactctatc aagatgattg tcatttactt
    12061 cgaggaaagt tgacatcact ttatccttta ttaaactttc tcttttaaaa tccatcacta
    12121 taacttttgc attttcttca gttgagttat attctgtaat atttagtatc tttaacatat
    12181 ctagtaaatt atctttttca taatctaaat ctttttcgat actctttttt ctatccttta
    12241 aatctaaaat tatacttatg ctttcatcta atttatcttt tatacctgca tctaatggca
    12301 tgtaatatcc cccatttata tttcaaaatc ttttttcgct gaatctatac tattaatttc
    12361 tgacctttta tcactttcta ctactaaagt agcttttcca actggtttta taattaaatc
    12421 acttagtagt ttagcaaaac tctttttacc tatagctttc tccatatcac taattccttt
    12481 taaggtcctt ggcttataaa ttttttcttc atcataatct gaatttaata aaaccttagc
    12541 aacttcttgc tcatctacat actttctatt acttctgcct tctacaagct tatatcctgg
    12601 atatttaaca ccatgctttt cagcttgttc tagtgcataa ctttgaacat cttttaacca
    12661 atcttgtata ttcttagcaa aacctaaaat atctgctatc tcatatttat taagagcaaa
    12721 tgtgtcagca aaatcatatt ttctagctag tttaaggtta tcctcagctc tttttctgca
    12781 atcattttta gctctacaaa atccacaatg actgccactt acaaactctc cttcaccatt
    12841 aaaagccatt tgagccttct tcttaacatt atctgcccat gtaagtaatt tagtaacttc
    12901 tatttcttca cttgatatgt tatcaagtct aggttgaatt attgttgttt taattaaatc
    12961 aatatcgtat agcatttcaa actgattata tgcccctaaa ccatatagtc taagttgagg
    13021 attctctata gctgaaactt ctaaaccttt tccatacttt aggtctataa cttgaagtat
    13081 accatcagat attacaacaa cgtctcctgt tccaaatcct tctggaaccc actcactaaa
    13141 atcgagtctt tcttctaaca tcactattac atcatcacat atagctttac tatcatttac
    13201 taattcaact acattctcaa cataagattg tatatagtcc tccatttcag agttataata
    13261 ctcacttttc tgtatctttt taattcttgc attatatgcc tttttactta ttttttcata
    13321 ctctagcatt aatttaactt ctgctaattc atgtgcaact gttccttctt ctgcatatat
    13381 actagttgat ggtggatagt tttcttctaa ttttatacta ggagtgcagt gaagccatct
    13441 atgggctcca ctcgcactaa gtcttgcatg ttgtaatggc atttatgtac cccctataaa
    13501 ctttctaatt tgttcataaa agctgaataa tcttcttctt ttacttcact taacttacta
    13561 gctccaaatt caccaaataa ctccttaagc ttatccttct tacctgcctt acttacttgt
    13621 gctgccttag ttcttacctc ttcttttgtg tatttaactt cactagttgt attttcatta
    13681 ttcttttcta tttcttcctt tgcttctgct atttcttctt taacgtcttc ttttacttct
    13741 acctttttaa tcttttcagc ttttttaact tcaactttct tctcttcttt catatctgtt
    13801 atatttatag gttctatctg cattgctttt cctagattaa gacctcctaa agcattcgcc
    13861 actactaata gtgcatttgt aaattctggt gcttctactt taacttttac atttacattc
    13921 acttcaacca ttttaaatct ctcctttttg tgttatactt tacttgtgtt atattttatt
    13981 ttttattttt ttgtgtgttg gttattttac caacactttt ttatttaata ttccaaccga
    14041 tattttctta ccagttttaa tatctttaaa tactatatct gctataactt ttccatcttt
    14101 tttaagagtt actacattct tattattagt atcaagactc agcaatttca tcccccctct
    14161 ctactgcttt taaaagttca tccaaatttt taccttgatt tctttcaata aaatcatcaa
    14221 cttcatatct tgaaattttt ctaccatcac ctctaactag tgattttatc aaacctgtgc
    14281 ttactaacct acgcataaaa gctgtatcta actttaaaat tccccttgct tcttctactg
    14341 ttattagata atttggataa cctcttttta ttaaaacaac tatatcttta ggctccagta
    14401 cctttacctt ttgttcaata gtttgactct taactctatc tgtctcttgt ttacttatct
    14461 ctatttctat taagcttttt aagctatcac taaattgttt tgttatgctt tcagaaatat
    14521 ccatgtgaaa atgctctccc ctcttttcaa ttatttatct tccaaccaat tcgtctaatg
    14581 taatatctaa ataatcagct aattttatta gagtatctat agttggatta gaacttttat
    14641 ttctcagtaa tttatataaa ccacttacat ctatatccaa ttcttttgcc aatttataag
    14701 gttttatatc tctttttctt agtatttgct ttatattgtc gcctattaac attgtattca
    14761 cctcagttct acatggtata atataaacat ggaataaatt ccaaaatttt caaaaggatg
    14821 gtgtattatg cagttttcaa acaatatatc taaagaatta caagaaagta taagaaagac
    14881 agtctctgat acttctaaca cagagtattt ttactatgct gaatttcaat ataaaattat
    14941 tcttaaatct atagaggaat ttgaaaaaga acttgatgat gaacatgaaa ttgcactaaa
    15001 attgactaac tttggaaaag atgtattaat gattgttgaa gaagtaggtt atcataaccc
    15061 ttgtctaatt cactactacg gcatagtaaa tggagtttat tctgaaattc ttcaacacac
    15121 atcacagata aactttatga taacttcagt aaagaaaact gacccttcaa aacctgctag
    15181 aagaataggt tttatattgt aattatcatt cttattttct gattctttct ttagtaattt
    15241 cactaatttt tcttttttca cttttattca tctcatgtaa atacaaaact atacattggt
    15301 acatataatc tataaaatca ctatttgatt gagctgttat ctgactaaat aactgtgctt
    15361 gcaattgatt ttcaagcatg gttattcttt tctctatttc ttctattttc ttattttcca
    15421 attcaatctc tcctttccaa aatattctgt atttagtttt caaagtacta acctaatatt
    15481 gaagtttgat aactatattc tttttctata tctggtaata tgttatttgt tttcaataaa
    15541 tcatataaaa acaatctacc tttttgagtc cacttagttg tcatttttac atcaggcatt
    15601 ccattacttc tagttatgtc tattgtttct gaatgagtgt atcccttccc ttgatgttgt
    15661 ttatataaaa gccattgtcc actttgttta tattgaatcc ctctttcatg aagtatttta
    15721 ttcatttctt ttccactcat tccatagtct tttgctattt gagttattgt gacaagtcct
    15781 ttgtttttaa gtatcatatc tgtataatcc gccttcggtt ttaattcttt tattacttgg
    15841 tctttcattt taccttctaa ttgtaactgc tctttctcct tttgttcctc tatccaacgt
    15901 ttagcacgct ctataggatt atctatcata taactatctt ttaattgtcg atttaccatg
    15961 ttatatgtgc cagttttacg aatacttggt agaacttcat ctgtaaccca atcttgaaat
    16021 ctctctgctt cttcttttct tgatttaaag attaacttat acacaccact ttcagtaaga
    16081 aaattttctc ctgtattgtg tagttttcta aagtcagtac tactgatttt agaattaatt
    16141 aacttaatta cttgtttatt attcatattt ctcaaatttt catttacatt ttttatattt
    16201 aagcactctg caacatgttt tgggttaaat aaaatctgcc cttcaaactc aaacacttct
    16261 acttcttttc cttcaaatac cattaagtta ttactcataa attctttccc ccttaattat
    16321 ttatagttga tttttctaaa agctcatttg atactttcat accatctgcc attgcagcta
    16381 tttattctgt ttttagtttt caaagtacta tcaatcttta gcctaataaa gttatttgac
    16441 actctcgttc tttttctcta attaatccat cttttataag taacaatcta aatgcttctc
    16501 taccttttgg atttattaat gtttgagtat ctgaatgccc gtatggtgtt gtaaattctt
    16561 tcagttcaaa gtactgcatc ttattagaat aaggttttat ctttcctttt aaatctctgt
    16621 aacagtattt cttctctatt aaccataaaa caaatacttt ttcttttact ccaagttctt
    16681 ttgatgtatc tcttatatta gttagtaaat ttctttctac taaagcatca aagtaatctg
    16741 ccttaggttt cattgtttga ttttctagtt gtagttgttc tttttcctca acttctatta
    16801 ataactgttg taatgcttct ttatatgtag ttggtaattt agtttgttca ttttttaaag
    16861 ctttttccat ttcatcaaat ttttttgtat atattcctgt aaatgctgta ccttttatgc
    16921 ctgtcatttt atttgctata aagtcacaac ctatctttgt tattgcgtaa catggtttct
    16981 ttcttttata atcatctaaa taagtatttt caataaaata atcagatggg tacaaatctg
    17041 tactcatgtt tttatttgct tcttccattt ggtttatata atttcttata tcccttagta
    17101 aaatcttgtg ttctttttca accattagag ctatatccct actatcagtt gttagtttat
    17161 tattaacttt tactattttt aaatctttca tatttacaaa ctcctctcta taaaatgtcc
    17221 atcattttat tgccatttta gcaactaatt ctgaaaaaaa atagtctgtt gatactccat
    17281 ataattcaga caactttttt atttcatttg ctttgaactc atttttacca ttaagtttaa
    17341 gcctaaaacc aaataaacta agacctaaca aatctgcaac aaatttttgc gtatgttggt
    17401 tttctttcat taaccctttc aatctattga catacatttt ttcaccctct aactttttgc
    17461 tatataagca agttcttgtt tatataatag caacaaaaat gttaaaagtc aatatttttg
    17521 ttgctttttt ggcaatttta attgtaataa caaaaaaagt tgctataatg taaataaaag
    17581 ttgctataat gaaaactaca ataaaggatg attttatatg tttagattaa aagaactgag
    17641 agaagaaaaa ggaatatctt tagatagatt aagtatggat ttaaatgtaa ataaatccac
    17701 tctatctagg attgaaaatg ggttaagaga gccaaaacaa tcttttattg aagactgttc
    17761 aaattatttt gatgtatcaa cggattactt acttggtaaa acagacataa aaaatcccaa
    17821 tcaagtagta aaatctttaa atcctatttt ctcaaaaaga ttaagagaat taataaagga
    17881 aaaaggtgtc acattaaata tattaggcaa aaaattagaa ataagtactg atttattatc
    17941 taaatatgaa aataatataa tttcacctaa aagtgatgat attataaagc ttgctcatta
    18001 ttttgatgtt acaacagatt atcttttagg tacaacactt gttagaaatc atatagacac
    18061 tgtagccgca cacaaagcaa atccacatga ggatttacct gaagaagctc aagaacagct
    18121 taatgattat attgaatttt taatgaataa gtataaaaaa taaattaaaa gtaggtgata
    18181 aattatatga aatctataat atcagatgtc ataaaccgat ttaatgctac accattttta
    18241 tttgttggtt ctggtttaac tagaagatac tataatcttc caaattggga ggatttacta
    18301 aaagtctttg cagaaaaaat tagtaatgat gactttatat atacaagtta taaaaataaa
    18361 gcaaaatcag agaacccaaa aatgggtata aatcctagaa ttgcagaact tattgaaaat
    18421 gattttaata aaaaatggtt ttcagattct tctatacgaa gcttagactc taagtattta
    18481 gatattgtta aatcaggagt ttctccattt aaggcagaaa ttgctatgta tattaaaaat
    18541 aattctaaaa tagtagataa atatcaagat gaagttgaaa aacttagtaa tatctctaaa
    18601 aaaagtttat caggatttat tacaacaaat tatgattgtt ttttggaaac tatcgttgat
    18661 aattatacaa catatatagg tcaagaaaat ttagtttttt catctataca aggtattgca
    18721 gaaatttata aaattcatgg atgtgtttct tctccaaata gtattgtaat aaatgaagct
    18781 gattatatag actttgacag caaaagtgcc tacttggcag ctaagctaat gactatattt
    18841 gttgagtttc ctatcatatt tattggttac tctgttacag atgtaaatat aaaaaaaata
    18901 cttaatgcta tagttaattg tctatctaat gaaaatgcta aaaaactcga ggaacgtttc
    18961 atattcattg agtatgaaaa aaactttaat gatatagaaa tatcaagtca tacaattgct
    19021 tttgataata aaatgattac tatgactaaa attaaactgg aggattttaa tttactctat
    19081 gaggcattat ctgaaaaaaa atcaaaatta ccagttagaa tattaagaat gttcaaacaa
    19141 gaattttacg atttcacaat tactaacaaa cctactgcta aaattcgggt tggtagtgtt
    19201 gatgatacaa gaataaaaga tgaagattta gttttagcag ttggcaaggc aagtgatttt
    19261 ggtcttaaag gattaaaagg attaagtttt gatgaatggt atcgtgatat agtcatgaat
    19321 gacttagaat tttcatcaga tgaattactt gaatatgctt atccatcttt aattaagcaa
    19381 tataataaat taccattaaa taaacatctt tttaatagca cacttgaatt tccagattat
    19441 aggacaattg cactagaaag tgactttgaa aatattatta gcaatagtat taaaaaaaat
    19501 agaaataata cttacataaa aaatagaagt gtttatggta tttggaatga tgaacatcaa
    19561 tcatttgaaa aagctacaag acttattgct tttttagaag aaaaagaaat tgacactgaa
    19621 cagttagaaa atctacttaa gaaaatattt gaggaaaatc ctaatatttt agagtctgca
    19681 aaaacaagcg aaaaaacaaa tttaagaagg cttattagaa tatatgacta catgaaatac
    19741 tctaatatgc aaaagagtct tggttatagt gagtaaaact ctacccaccg ctccaaaact
    19801 cctttaatcg ctttaagcac aaatacaaat ttagtgctaa ctatactaac acacatttgt
    19861 aagtgcttac atatattata tgtaattata ttataatcat atactataat ccatattttg
    19921 tctacagcta aaattatcca atcttttttg gataaaaaat gaatatttag agcagttcac
    19981 acctgctctt tatatataac aaaaatcaaa aatacattct ttttataggg ggatttcaat
    20041 gaacaaacta gacgcacttt tagacttagc aaataatgaa gagatagaaa tttactacac
    20101 tgacaaaata gcagatgaca taaaaggatt gtatataaac agacaaggac taaagattat
    20161 atcattactt aattcattaa aacaaaacaa tgctaaacta atagaaatct tagcagaaga
    20221 attaggacat cattttacca gtgttggaaa ctatgtatct tcaaaaaaca gttacaaaaa
    20281 taaaatcttg atagacaaaa ctgaaaacaa agcactaaaa tgggcatgtg aatttcttat
    20341 aacagaagaa gaaataatac atgttattaa ttcacacgct acaagtgtat acgaaatagc
    20401 tgaagaatta caagttagca tcaacttctt actaaaaaga ttagaatttc tatcaaaaaa
    20461 gaaaagcatg ttggacttag gaaataatag atttttagta ttaactaatt tgccaaattt
    20521 ctacatatat gaggatattt tttaaactca tttattcta
//
LOCUS       DACXMZ010000050        20524 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.050, whole genome shotgun sequence.
ACCESSION   DACXMZ010000050 DACXMZ010000000
VERSION     DACXMZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.050"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            81..1004
                     /locus_tag="JD016_07240"
     CDS             81..1004
                     /locus_tag="JD016_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAU4999433.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(935..1162)
                     /locus_tag="JD016_07245"
     CDS             complement(935..1162)
                     /locus_tag="JD016_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999434.1"
                     /translation="MYLILLVKWGYLKIEISLFFSAEMMNIYFTLYKKECLIQCKVHF
                     ETLSLFIMIYFSLGVSNLDVSNMIEEKSFCI"
     gene            complement(1176..2318)
                     /locus_tag="JD016_07250"
     CDS             complement(1176..2318)
                     /locus_tag="JD016_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAU4999435.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(2336..4090)
                     /locus_tag="JD016_07255"
     CDS             complement(2336..4090)
                     /locus_tag="JD016_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HAU4999436.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            4226..4432
                     /locus_tag="JD016_07260"
     CDS             4226..4432
                     /locus_tag="JD016_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999437.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(4437..5360)
                     /locus_tag="JD016_07265"
     CDS             complement(4437..5360)
                     /locus_tag="JD016_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999438.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDHDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIADPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(5614..7314)
                     /locus_tag="JD016_07270"
     CDS             complement(5614..7314)
                     /locus_tag="JD016_07270"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAU4999439.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(7724..8137)
                     /locus_tag="JD016_07275"
     CDS             complement(7724..8137)
                     /locus_tag="JD016_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAU4999440.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(8180..10558)
                     /locus_tag="JD016_07280"
     CDS             complement(8180..10558)
                     /locus_tag="JD016_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAU4999441.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     ISQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKAEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKSGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            10877..12328
                     /locus_tag="JD016_07285"
     CDS             10877..12328
                     /locus_tag="JD016_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HAU4999442.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(12532..15030)
                     /locus_tag="JD016_07290"
     CDS             complement(12532..15030)
                     /locus_tag="JD016_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAU4999443.1"
                     /translation="MNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVAYPIQK
                     GLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENGEIDIM
                     GGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVIENSDT
                     RLKELDEYCKMNLMSPELVYCKTNDEQLEVLKSGKADVLLNVSMNPIEGVRTVAKFSS
                     KPFYFITPKGSTDLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDEETNYI
                     KETEKLKVGVLLNKPPFQNADKDTGELRGISIDLLNHICDKTGLEFELVPVKSQEDLE
                     DMAKKHEIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLNGKRIALT
                     KGVNYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNVRLVPQ
                     TYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLYIMQQN
                     PIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFEYDYKQ
                     DKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQENGTTE
                     MYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQRDSLTN
                     IYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDVAKVLD
                     ESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLSISMGV
                     VIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(15268..16089)
                     /locus_tag="JD016_07295"
     CDS             complement(15268..16089)
                     /locus_tag="JD016_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAU4999444.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSMYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(16607..17083)
                     /locus_tag="JD016_07300"
     CDS             complement(16607..17083)
                     /locus_tag="JD016_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HAU4999445.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(17117..17695)
                     /locus_tag="JD016_07305"
     CDS             complement(17117..17695)
                     /locus_tag="JD016_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAU4999446.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(17799..20399)
                     /locus_tag="JD016_07310"
     CDS             complement(17799..20399)
                     /locus_tag="JD016_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAU4999447.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHSNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT         6455 a         3690 c         2292 g         8087 t
ORIGIN      
        1 taaatactta actatactta aaaagcatat aaattgatat aattatataa tagataagtt
       61 aatattgagg agggataatt atggaaataa gagtattaag atatttttta gcaattgcaa
      121 gagaagaaag tatatctggt gcggcaacct ttttacatat aacacaacca actctttcta
      181 gacaaattat ggaacttgaa gaagagttgg gagttaaatt attggttcga ggaaatagaa
      241 aaatcacctt aacaaatgaa ggtaggcttt tacataaaag agcagaagaa attatatcat
      301 tagttgataa ggttgaatta gaatttgaca atcccaatga gattattagc ggtgatatat
      361 atataggaag tggtgaaact gaagctatgc gacttatagc tagaactgcc catcattttc
      421 atcaaaaata ccccaatgta agatatcacc tttttagtgg gaatgctgat gatgtgactg
      481 aaaaattaga caagggactg cttgattttg gtattttaat tgaacctgca aatatacaga
      541 agtatgatta tgcacaaatt cctgctactg atgtatgggg gttactaatg cgtaaagata
      601 gtgcccttgc atcacaaagc acaataaatc caaaagattt atgcaatata cctcttatta
      661 tttcaagaca gacacttgtt gataaagcaa tttctaaatg gcttcaagac agtaatgaaa
      721 aactaaacat tattgcaaca tacaatttag tttataacgc ttctcttttg gtagaagaag
      781 gtctgggcta cgctctgtgt ttagatagac tcgtcaatac tacagaaaac agtaatcttt
      841 gttttagacc tctatctcct cctttagaag ctaaattaaa tattatatgg aaaaaacatc
      901 aaatattttc aaaggcagca gaaaaatttt tacttcaaat acaaaaagat ttttcttcta
      961 tcatgttgga tacatctaag ttggatacac ctaaagaaaa ataaatcata ataaaaagtg
     1021 agagtgtctc aaaatgaact ttgcactgta taaggcactc ttttttatac aaagtaaaat
     1081 atatgttcat catttcagca ctaaaaaaga ggcttatctc tattttgaga tagccccact
     1141 taaccaataa aattaaatac aacttaacaa tatccttact tatttattcc aagttctctt
     1201 agacaagcaa tagtaatttc atcttgtgga tttaattcat aagctctttc tatatagtaa
     1261 gttgcatcat caatatcacc attatttaaa taagccattc ctaaattgca aagtatttct
     1321 gggtcttcct ttatcttagc cgcttgttca aaatactcta ttgctttatc taaattattc
     1381 ttataagcct cacaaagccc caattccact atagtatcca cttgattagg ctttattatc
     1441 aatatcttct caagatacat cttagcctgc tctatttcgc ccataccctt atatccaagt
     1501 gctatcataa acagcaagtt ccaccaatcc atatgttctt cttctaatgg aagtaacttc
     1561 tctaatcctt ccttaaactt accttggaat acaagtgtat atccttcttc atattgaact
     1621 ttaaagtcca tcttgcccag attctcttgt acctcagcta ctaaatcagc atctaaccct
     1681 agccttaacg cttcttccca agtcagctta gtctttaaat attgaccttg attataataa
     1741 tggtagccta agtgataata acctaatgca aaattctcat caacatcaat taccttttca
     1801 agcttagcca tggcttcaag taagaatgca ttcattgcct tatcatccat atctttttga
     1861 tactgtttag ctatctcttg gcaaacaatc gcatagttat acattgcatc taaatcatct
     1921 ggatacattg ttataaatgc ttttatatat attaaagaat ctgtatgttc accaatctca
     1981 aagtattttc tcgacatata tcccatatac gatttcaaat ccaaatcaat atcttttgat
     2041 aaagcattta aaaacttctc atactctgca ttgtttttaa acttactatc tattccaatt
     2101 atatatatca ttgcatctgc tatagacatt gaatttaact tatcttgagc agtcttttct
     2161 tttatccctt ttactaatac ctcatttttt ataggtacat ctaatccacc ttttggcact
     2221 tcatagcttt ttaatttaaa ctctccacca tgttttatag ttataaaagc taattcttct
     2281 gcttttttta gcaaatattt ttctatctta aaatccattt tatcctccta aatacttaaa
     2341 tctctacttt atgagtttta gatttctcaa atattttttt atcatcatac tcaaataaga
     2401 ctacattttc tacttctctt atttctgtat ttatatcatt ttttaaatcc acaacattaa
     2461 atctaaatgg ctcaaaatga acatatttta ttatatgttt agctggttta tcaacaaaac
     2521 taggtaaata tgttgataag ttatcattat cttgtaaaaa tacatggcat ctattcttaa
     2581 aatcatccac atctagctta ttaaaaaact gtggtatatt tgatgtttta cctagtgata
     2641 tatagtcata ttttaatata tcgttgaata catcattatc tatgttgatt ttttcattat
     2701 agaaatccat caatatttta tataactgat tctttccttg tgccaaatca aagtagccat
     2761 tttcatcaaa atacttggca aaaacctcaa agaatttaaa agggcttttt ttataaaatc
     2821 tgcctataat atatctcata gataacacaa agtttttaga attgtagtat ctctccagta
     2881 tatcttctat atcctttagc ttcaaagttt cttcatagct tatataatta ttgtataaaa
     2941 actcatatgg agcataatcc ttatatttaa aaccatgctc atctgcaact tttctcatac
     3001 ctgtcccttt aatcatcttt aaaaagccta attgcagatg ctctatacct agattaaata
     3061 catcattaaa agaattttca aagctcttat aatcttcata aggcagtcct gcaattaagt
     3121 ctaagtgctg atgtatattt ctataagatg ctacagtttg tacaacatga gacaactttt
     3181 taaaatcatc ccttctgcct actgcatcaa gtgttttttc attcgttgac tgaaccccta
     3241 tttcaaattg aaaaagacct tctttacaat cagccaaaaa ctcaagcatc ttatcatcta
     3301 tcaaatacgc tgtaacttca aaatgataag ttgtatatcc attatcattt ttcataagga
     3361 aatccatgat ttccatagcc acttttctat ttgcattgaa tgtcctatct ataaacttta
     3421 tttgtgatac tcttgcatct attaaagctt ttaaatcact ctttatcctg tctaaactaa
     3481 aataccttag acctggaata gtagatgata aacagtattg gcagttaaaa gggcaacctc
     3541 ttgatgattc ataatacact attctatttt catattcttt tggattcaag ttttcataag
     3601 gactaggaat ctcatccaaa tcttgaagta attccatagg cttattcact attatctcat
     3661 tgccttgtct atatacaagt ccatttacat catttatttc catctttcct tgcaagtgaa
     3721 gtactaagtc tctaaaaatt aattctccct ctccataaag tatataatcc acaaaatcat
     3781 actttttcat tgcctcataa ctatcataac tcacttccgg tccgcccaaa gctattttag
     3841 tattaggtct aatttttttt agattatcac aaagtttaac tatatctccc acattccaga
     3901 tatatgttga gaataatatt atatcatatt catttttata tatatccttt aaaatatagt
     3961 ccaaatcgtt atttatcgtg tattctttca tattaacttc tatcaaatct cttacaaatt
     4021 cttttagata tcttatagcc aaatttgtat gaataaactt ggaatttaat gttgttaata
     4081 aaattttcaa atatttctca cctcttttta agattatact gtctttttgt accccttata
     4141 ggttaaaata gtatatgtaa agtctaaata atatctatat aattaattta ttaatttcaa
     4201 gtaatgtgag gaaaggaagt tttttatgga attaattgaa aaagtatcta tcaactcttt
     4261 atcaaaaaga gatttactac ttattataaa gtctcttgaa tacactaatg aaaatactca
     4321 aattagtgat ttcattgacc ttaggaataa tatagtaaag gaattatgtt ttttaactga
     4381 cactaaggaa aaagattttt tagattatct agaaaaaaat agcacactct aataacctaa
     4441 acctttttaa actttaatat ataaatacct gatttagaat ttatttttat gcttaatata
     4501 ttatcattaa aggttttcga cataactgtt atttgattaa atatagggtc tgctatagta
     4561 ttaccttctt caagtcttat aagacttctt gggtctatgc ttttattata tgccttaact
     4621 ccaattttat attttctcaa tttaacaaca tattcggaca tagaaaaaat aaaatctata
     4681 ttatcttcat ttaatttact atcttcaccc aaaatggata atatagattt ttttatgcta
     4741 ttaattcctt cattttctac agttctatct tccatatata catcatatag aacatttaaa
     4801 acatttaata aaatactttc ttcatcatga tcttcagatt tcaaatcaat gtacttatca
     4861 attaattgta ttgctttaat ccttgccatc tgaaacttta caacatctga tttgtctaat
     4921 tctatagaaa atcctattat cttgtcttta atactctgta gtaattcgac atactctatt
     4981 ttactattat ttataagatt gtgcataatg gaattataca ccacagaacc taataaataa
     5041 tcaaataaaa gttcttcttt tttaaaataa ataacatact tttttattag tttaataacc
     5101 tcatcttcac ttttttgaat attagtattt gataaaatta gtggtaataa aatatttaaa
     5161 aaattgatat ttcctatttt ttctccataa aatctatagt aaataagttc atctctcagt
     5221 ctattttcgt ctatatacac acatctttgc gttttatcat ctattaagga ttttgtattt
     5281 attatagtta aatctttaag agccctactt gcactactaa aagaagatgt gtagactttt
     5341 tttagtaatt cgtcattcat aaatatatcc tttctgaaaa attgaatgta aataaattgt
     5401 tattaaaaaa aaagagacag cccgtttaag aagctgtcat atataggggg atagtagtgt
     5461 acaaaaatca ttgtaatcta ctatctatat attattatac gatactactt ttttatatgt
     5521 aaagcatcag ttttcgacat ccctatattt tacagttata caaattgcgt actaagttca
     5581 attttattct ttttatttca acattttatc atgctacatt ctttcaggag cgtgcattcc
     5641 caacaatttc agagaatttt ctaaagtttg ttttgttgct aaaactaatg caagtcttgc
     5701 ttttcttatt tccaaatttt ctactaaaat tggattgtca tgataaaatt tattaaacgc
     5761 ttgagctaaa tctagcataa atctagtcac tatatgtggc tcatttttct ttagagcaag
     5821 taaaatattc ttattgaatg attctataac tctaagaact tctgcactat cttcatcagc
     5881 taatagacta taatctatat cagaagtcac ttctacttct gcttttctta aaactgcaca
     5941 gcatctagca tgtgtatact gaacatatgg tccagtctca ccatcaaaac taagcgttct
     6001 ttcccaagag aaggtatagt ctttaattct actattagat aactcttgga atactactgc
     6061 cccaacacct acttgcttag atatttcatc cacattttta gcatttgggt ttttagctag
     6121 tataatttcc ttagttttat ctatagcttg atttaaagca tcttctaaaa atactactct
     6181 tccttttcta gtcgacatag taccttcttc aagtgcaacc ataccaaatc caacatgaat
     6241 taaatctttt gcccaatcat atcccataag ctcaattacc ttaaaccatt gttggaaatg
     6301 taagttttgt tgagaaccaa ctacatatat acacttgtca aaatcataag tttcttttct
     6361 atatattgca gcagctaaat ctcttgtcat atataacgtt gaaccatcat tcttagtaat
     6421 aagtgctggt ggcattttat actcttctaa atcaacaatt cttgctcctt tagactcttt
     6481 taaaagattt ttctcttcta atagttcgat aactctgttc attttatcag aataaaaact
     6541 ctctcctgct aaagaatcaa attctatatc taaaagttta tacactctgt taaactcttt
     6601 taaactctca tttctaaacc attgccataa ttcttgtgct tcttcgtcac cattttctaa
     6661 tttattaaac catgctcttg cttcatcttc catttctggg tgttgttcag cttcatcatg
     6721 gaatcttacg tataatttta gcaattctgg aattggatta gactctacta cttctttttc
     6781 tccccacttt ttaaaggcca ctattaactt accaaactga gttccatagt cacctaaatg
     6841 atttattctt atagtcttgt atccttgcga atcatatatc ttatataaag cattacctat
     6901 aactgtagtt cttatgtgcc ctatatggaa aggttttgct atatttggag aagaatattc
     6961 aactattaca gttttatctt ttccaaaatc actatgtccg taattttctt tttcgtctaa
     7021 aactttttta attacagttt ctgctaattg agatttattt acaaagaaat ttacatatcc
     7081 gcctaattgt attacttttg ttatctctcc acttggttct atactttccg ccaattcact
     7141 tgctatcatg tttggagctt ttctaaacac tttggctaat ttaaagcatg gaaaagcata
     7201 gtcccccata tccttatttg gtggaacttc tataagtgcc tctatctctt ctttcgataa
     7261 atcttctatt ttttctttaa ggcaatttga tattgccacc ttaaaatctt gcattctgtt
     7321 gctcctctct acattgttcc tggtttatat tgtgcttatc tctttatgtt aaatcaaatt
     7381 tcgtgttttt tcaagtattt cattacagtg taaaacactt tagtaattat gttttctgta
     7441 tatattaaaa atactataat tgcagttttt aatcacacta ttattgattt tttataagac
     7501 ttttttctat tttattgttt cttgtactat ccagttgact ttatatactt tttttactct
     7561 tttatctgtt ttcatacatc aatgtcagct ttatatacat ttttttaatc ttttatctat
     7621 tttcatacat caatgtcagc tttatatact ttttttactt ttttatctat ttttatacat
     7681 caatattagc tttatatact ttttttactc ttttatctat tttctataaa tctttttcac
     7741 tcttaacttt tattccttct tcttctaata gccttgctgt tatacctttc cctggtatct
     7801 ttttacctga aaaactacca tcatatatta gattacatcc acaagagggg ctaccctctt
     7861 ttagtattgc ttcgctacat acatataaat ttgctatttt tagagtttca ttagcccctc
     7921 ttacaaagtt ttctgttaca tccttattat ctttagtaat tacttttcca tcaactatct
     7981 cagcaggtgg tcttggtgta gatagaccac ctaactgctc tgggcataca agtgtaaatt
     8041 ctttgccttt taaatattcc ttgacttttt cattatcatt attatcgcca ctatatttac
     8101 aatttattcc tattaaacat gcagatacca atatcataaa aatgtgctcc tatacaaatt
     8161 atatatattc tcatacaaat tattttaatt taacaattgt tactcctgct ccaccttctc
     8221 catattgacc acctctttgt gattttacat gtctatttct tcttaaaatg tcttgtagcc
     8281 ctgcttttaa tactcctgta cctattccat gtattactgt tacagactct aaacctgcca
     8341 catatgcatc atcaagatac ttttctactt ccattatagc ttcttctagg tttaaccctc
     8401 taaggtcaac ttcatttttt acactaccag attttgacct tataatgttt ctcgttgatt
     8461 tagttacatt tgttgaaaca tctttgcgtg ttttttgtaa agatttaaaa ggtagagtca
     8521 tcttcattat accaatttgc actactgctt cttttctctt tttatctact gaaactacac
     8581 taccattttg atttaaagtt attaccttaa cttcttcacc tggctttaag tcttttatct
     8641 ccttatttga tacctttgga actatcatac tttttactgt tggctgaaga cttcccatag
     8701 agctagtcaa ttcttttcta agttcttcta tcttacgatt cttttctttt gaagctcttt
     8761 cttgttcaag acttctaagt tcttttatta taatatctac ttcttcttta gcttgtctta
     8821 taattgaaaa tgcttctgat ttagcttttt caatcatctt atctctttga ctgaccaatt
     8881 tttcaagttt ttcgtcatac tctactttca gtttttcaat ctcttctttt aatctttcag
     8941 cttcttctct atcttctact gctttgattc tattcttttc aacattctgt aacacatctt
     9001 ctaaagctat gttttctgta tttatatatt ctttagcacg tgaaatcaca taatcactta
     9061 gcccaagttt tctagaaatc tcaaacgcat ttgattttcc gggaacacct ataagtaact
     9121 tatatgttgg gcttaaagtt tctatatcaa attcaactgc tgcattttct acacctgatt
     9181 tagtaagagc ataattttta agctcactat agtgagtggt agctatacat ttagctccta
     9241 cagaatttat atcttctaat actgctattg caagggcagc tccttcaaca gggtctgttc
     9301 ccgctcctaa ctcgtcaaat ataactaaag agtctgctgt tacgttttgt aatatagata
     9361 ctatattcgt catatgagat gaaaatgttg atagactctg ttcaatactc tgctcatctc
     9421 ctatatctgc aaatacatta tcatatacac acatactact tccataatca gcaggtatat
     9481 gaagtccact ttgtgtcata agtgcaaata gacctacagt ttttattgta acagtcttac
     9541 cacctgtgtt tggacctgtt atcaccaatg tgtggaagtc tcttcctaaa taaattgtat
     9601 ttgctactac cttcttttta tccaacagtg gatgtcttcc attttttatg tttaaatatt
     9661 tatcctcatt taaagttggt tctattcctc tcatctgtat agaaagtttt ccctttgaaa
     9721 atgcaaagtc caatcttcca agtatctctt gattagagat taaatcttcg ctcacttctc
     9781 caaccatagc agaaagctct gataaaattc tttctatttc ctcttgctct cctagtttca
     9841 actgcctcaa ttcattgttc atctctacta tagtcattgg ctctataaaa agtgtcgcac
     9901 cagaagatga ctggtcatgc actataccag ctacttgcga cctatattca gcttttacag
     9961 gtactacaaa tctatcacct cttagggaaa ttattgcatc ttgtaagtac ttttgataag
    10021 tagttgaact tattattgaa tttagttttg acctaatgga ttgatttttt tgagatattc
    10081 ttcttctgat atctctaagt gtacttgaag cattgtcaga tatttctact tcactaatta
    10141 tagcattgta tatctggtct tctatgtcct tgtatacata tagagaattt gatagagatt
    10201 gtattattgg ataattaaaa tcttcttcat cactactaga taagctattt cttaatcttc
    10261 tagccactct taaggtatct gctatcataa tcagacttcc tgggtcaaga gaagcaccta
    10321 tataagctct ctttacctta tcttctatat catgaatacc ttccaaccct actgaacctc
    10381 tctttataat aattgattga gcttcactag tttcttctag catagatttc acttctacaa
    10441 aatcagtatt tggtactaaa ttttctatat attttagacc taaagaagat gaggctttct
    10501 tctttaacaa atctattatt ttattgtatt ctagaactct taacgatttc tcattcattt
    10561 tctatattca ccatcctcga tattttaact tttttattta attttaaaaa tgtatgtttt
    10621 caagcatttc caataaaaac tgcaagtttt atttcttttt catttctaaa attattatat
    10681 tttttctctt ttagatatat tttacacata ttttgtatta tactatgtat gtagctatct
    10741 atatattata cataaacatg ctttatttgt aatataatct gttaaaaaat cttctttaat
    10801 atttttaaag gagtttattt tagcttataa aaaaaaatag ctacttatat agataaatta
    10861 aggaggaaca tgagtcatgg gaaacgtatt agaaggatta aaaccagaaa gtgtttttaa
    10921 aaactttgag aaaatatctc aaattcctag aggttctgga aatgaaaaag gcataagtga
    10981 ttttttactt agctttggta aaaatttagg tcttgaaact attcaggatg aaagcttaaa
    11041 cataataata agaaagcctg ctacaaaagg atatgaaaat tgtccaggtg tagttcttca
    11101 aggacatatg gatatggttt gcgaaaaaga aaagaatgtt gaacatgatt ttcttaaaga
    11161 ccctataaaa ttaagaattg atggtgacat gatatatgct actggaacta ctctaggagc
    11221 tgacaatggt atagctgttg ctatgggtat ggctatatta gaagataata ctttagagca
    11281 tcctgcacta gaagttttag ttactgttaa tgaagaagat ggtatgaatg gtgcagatgc
    11341 actagaccct agcttaatta aaggtcaata tatcctaaat atggactccg aggaggaagg
    11401 atacttatta gttagctgtg ctggtggtaa aacttgtgtt gttagtttac ctgttgaata
    11461 caaggaagtt aaaggtgaca aacaaggatt attagttgag gttactggac tattaggtgg
    11521 acactctggt atggaaattg ttttacaaag agcaaatgct aacaaagcaa tagctagagt
    11581 attaagtgta ttgaatgttg attatgaatt agcttctgtt gatggtggta ctaagcacaa
    11641 tgctatacct cgtgaagcta aatgtgtaat agctgttaat aaagctgatg ttgaatctgc
    11701 taaaaaacaa ataaatgaca ttttaactgc atttaaacat gagtttacta cttctgaccc
    11761 tggcatgact tactcagtag ctgaaacttc tgtagacaaa gttcttacta aagattgtaa
    11821 agaaaaagtt gttcaaatgt cttgcttaac tcctcatgga gttcaatcag taagtcttga
    11881 tatagaagga ttagttgaaa gttctactaa cttcgctata attgaaacta aagaatctac
    11941 tattgaattt ttaacttctg ttagaagttc tgttatgtct attagagatg aaatcgctga
    12001 tagaataaga cttttggctc aagctttagg tgctaattac gaccttatag ctcaataccc
    12061 agcttgggaa tttaaaaaag gctcaaaatt agaaaaaata tgcagtgaaa cttatgaaaa
    12121 attaactggt aaagttccta ctgttatggc tctacatgct ggtcttgagt gtggattatt
    12181 acttgataaa cttcctcatg ctgaagctat ctctatagga ccagatatgt ttgatgttca
    12241 tactccaaat gaacatgtta gtatcccttc tgtagctaat gtttgggatt atgttattga
    12301 aatattaaaa tctatgaacc aatattaaat ttaagttttg attctaagta gattttaatt
    12361 ttaagtaatc tatataaaat taactctgta aattaatact actgttagta taatatttac
    12421 tttcaatatt aacttataga aataaagcct aatgatagat aataacatgt atcattaggc
    12481 tttattgtaa tttaaatatt attaagcttt attctgtttt aaaattaatt cttatactat
    12541 tttaaatcta tttcttcctt gtctcttaga atcataaaga gcattatctg ctatttgata
    12601 cagttcatca tactcttgac caggctttgc tataactacc cccatactaa tagataaact
    12661 ttctccattt ggcaacttaa tgagatgaat atgattgtat acatcattac atttattaat
    12721 aagttcttcc ttatttatta tatcattgat ataaacacta aattcatctc cacccaaacg
    12781 acctactata tcattttttg caaaactttc atcaagtacc tttgcaacat caataagtac
    12841 tttatctcct aacaagtgac cataagtatc attaatcgac ttaaaataat caatatctat
    12901 aatcaataaa gcaccatttt tttcagttga taaattcaac aaattttgtg cagttaattt
    12961 tctaatatat gctgcattat aaatattggt taaactatcc ctttgagcct tatctaacaa
    13021 tctattcttt tcctcttttt cagaatcaat attacatatt ttcccgatta catacattgg
    13081 tatttttcta tcattataaa ttatttttga agtaactcta atccaatgaa aagtatcatc
    13141 aagcataata gaatacattt cagttgttcc attttcttgt gattttatta catcaaaaaa
    13201 ctttttatta tattctttcc ttattttatc tctttcattt tctatattgc cattttcact
    13261 ttccttatca tttttatcca aaccataata agtttcaata cccttttctg gcatagaaat
    13321 tatcaattta tcttgcttat aatcatactc aaaaaagtat tcacttgtca tttcatacaa
    13381 ctgagtatgt tttttgagtt ccaaagatat cttctcgttc atacttgtac gagtatgtaa
    13441 accaaatgcc ataaatccaa taatacaaat taataaactg gctacaataa aaatagtctc
    13501 aattggattc tgttgcataa tataaagcaa cgagaaatct tgtctatatg tagtattttg
    13561 gtagacaatt gattgcattt tttctgatga aatacttata atagctttat taaatatatt
    13621 caataattct tgtttatctt ttttaattat accaaaacaa atactctttg ctttataggt
    13681 ttgaggaact agacgcacat ttttatactt aggttgattc atatagtact gcatagaata
    13741 accttctcca taagtgtaat ctgcatagcc tttattaaca gcttctatac actcttctaa
    13801 attatcatac caaactatat tctcttcaaa ctggccataa taattaacac ctttggttag
    13861 agctatacgt tttccattta gactattctc atttaattta ttattaatca tcatagtata
    13921 ttgagtagaa agaaaaggcc gagacatagc tatattatgt tcacgagagt actcataatc
    13981 ataaatcatt cctgctacaa tatcaatttc atgtttttta gccatatctt ccaaatcttc
    14041 ttgagatttt acaggcacaa gttcaaattc caaacctgtt ttatcacata tatgatttaa
    14101 caaatcaatt gatatacctc ttaattctcc tgtatcttta tctgcatttt gaaacggtgg
    14161 tttgtttaaa agtactccta cttttaattt ttctgtctct ttaatgtaat tagtttcttc
    14221 atcagataaa aacatttgat tattctctgg cgtaaagtat ttctcatata aagtagttga
    14281 aaaatatggg tccttctgtt caatattatt aattgctgaa ttaagctttt gagttaagtc
    14341 tgtactgcct tttggggtaa tgaaataaaa tggctttgat gagaattttg ctacagtacg
    14401 tacaccttct attgggttca tactaacatt aagaagtaca tctgctttac cactctttag
    14461 tacttctaat tgctcgtcat ttgttttaca atataccaat tcaggtgaca tcagattcat
    14521 cttacaatac tcatctaact ctttaagcct agtatcagaa ttttctatta cagcaatacg
    14581 gatttttttc acatcttgtg aactcattat gttatcaata tctgtatttt catataatgc
    14641 ttgtagtact gtatttgcta ctccataatt attccctgca taattatata ttttttgcat
    14701 tggctcattg tataacatcc ctcccattat atcaatttca ccattttcta acatcttcat
    14761 taattttgta atagattcat ttatctctcc aggaacttga acaaattcat aattccatcc
    14821 agtatactgt gcaatttcta gtaaatattc atatgtatac ccaaaatagt taccttgctc
    14881 atctatttca gttaaacctt tttgaatagg ataagcaaca cgaatggtac gattatcatt
    14941 acctatagcg ttgataccaa aagaaaatat gcaagaagtg aatatagatg taattacaat
    15001 aattaataaa agtaattttt ttaaattcac tacacaatcc tccatatagt taagatttac
    15061 agtataaatt actgcataaa cttgtagaca tgaaaaaata gattagtata taaaaggata
    15121 tataaatcta cttagagatt taaatatcct taatatatac aatatattta aattataact
    15181 catttatatg tctattacaa caaatttacc ctgctaaata cctactctat atcccataat
    15241 tttacaagtt gtagtgaagt ttaagttcta tctcacctca aatttagcag tgtttctatt
    15301 tacagttctt ttatatttca catctggata acccaatata aatgaagtga taactgcttc
    15361 gttctcgctc attcctaaca gttcttttat ctttggctct acatttgatg ccataacaaa
    15421 aaatccatta taacaaattc ctaatccttg agaataggcc ataagttcca tattagatgc
    15481 agccaatcct ccatctacat aagcacttcc acctagagac atatcaccta caactaccat
    15541 aagtagtggg gcatcataaa ataaagaatc atttccgttc tctttgtatc ttttgtacat
    15601 tttcttaaat gtatttttat acatagaacg tacagggtca ttgtcatcag tatctaatgc
    15661 caaattataa agaccttgta ttgccagttc tttcacttct tttagctttt cttttactaa
    15721 tatgtatctt ataggctgtc tattaccacc tgttggtgtg tatctaccag cttccactat
    15781 gttttttatt ttttcttctt ctacctctat atctttatat tgtcttatac ttcttctaaa
    15841 tttaataaaa ttcaataact tttcgggttc taattcaaaa ctttcacgat tgtattctat
    15901 tacttcattt ttatcaaatc cttgcatttc tattgcatct gttgggcaaa ttgccataca
    15961 gtgaccacag ttaatacaga gaatattttt aacctttgct ttatcatcct taacttttat
    16021 tgcatttaca agacagtctg attcacacat cccacagcct acacattttt ctaaatttat
    16081 gtctatcatt ccaaattatt cctcctttta aaaaatttac tttagtataa aacaaatagt
    16141 tgtctaagtc aatttttatt tttttaacaa tatactagcc ttaactacac tttaatggag
    16201 aaaaatatac ataaattgga acttaaatta gaacttattc aaagtaaaag ataaaagaag
    16261 tagatgtcta aaagtgaact ttttagttaa ttagatattc tttattgtat gaaatcaagt
    16321 ggatatttat catttcaaca ataaaaaagg cttacctcta ttttggatat gtccctgtca
    16381 agtagacata ctaaaaaaag gctatcttta tgcactgaga tagccccttt tcccaactac
    16441 tattgtttat cttaatatta gtcaataaat ttaatttgct ataacctgta agttacattt
    16501 taatttgatg taactttaaa ttacattttt aagtaatatc tttaaattat attcttaaaa
    16561 tatatcatta aactacattt taattaatct aaaaactata tcttaattaa tctaagaaac
    16621 ttacattata atcttttagt ttcccattat catatacaat ctttgcacta aagatatttc
    16681 cagtcttagc ccaatttaag aacagcctgt caccttccca catatttaaa tcgaatattt
    16741 tatcattatc aacccaaatt aaatttcctt ctttacaatc atttaaatct ttttcagata
    16801 attcaccttc aaactcatct gagaaataca aaaacatata ccaatcttct tcatctttaa
    16861 attttggaaa agttatcaac cctttatatg acaatgattt aagagtaaga cctgtctctt
    16921 cttttacttc tctaattaca cattcttctg gagtctctcc ttgctcaaac tttcctccaa
    16981 ctcctacata cttaccttca tgtatatcat ctttcttttt atttctgtac aacatcaaag
    17041 tcttgtcatc tttttctata taacaaatag ttgtaagttt catgtatctc gccttgcttt
    17101 ctctttatat tctcaactaa ttattttcag cactttcaag cacatcactt atattcttca
    17161 ttgcattaag agaaagctct gcaaattcat caaatggaat ccctatatct tctatacttc
    17221 tcattgcatc tctatttaca tttgaagcaa atctcgtttc tttcattctt ttaataacag
    17281 acttagtctt tacactggta agttttttat ctggataaac taaagctata gcagttataa
    17341 acccagttat agggtctgct gcatatattg ctttttccat taggctagtt cttgtaactc
    17401 ccattccatc atgatgacca agtattgctc tatacatttc ttcgtcaccg aagttttctt
    17461 ttttaagcag ttctactgtt gttgtagcat gtccttcaca tatttctcct ggctttcttc
    17521 caactatatc tgaatccaaa tcatgtaaaa gacctgccac tgcccactct tcttcttttt
    17581 ctggctctag tcttttggct aattctctca taacagcttc aacagcataa caatgttttc
    17641 tcattctatc agtttctaaa tacttaaata aaatttctaa agcttcttct ctcattttca
    17701 ccaatccccc tgttttttca catatatgat atttttatat attaatacac ataaattcaa
    17761 gtttttatat atattcatgt atatttttca tgttatatct attctacacc tttatagtag
    17821 tgtcctgcaa ctgttccaat ttcatctaat ttcttataca agtctgtagc cttttttcca
    17881 agtttataat tattactcaa tacctcaatg tgagcttcaa taaccttttt aaccatctca
    17941 ctatcttcaa ctgtaaaatc tatcctaaat acatctattc caatttcctc taactcatat
    18001 agattgtcca acatacaagt aaccttcgaa ttgtaaattg tacttctaca gaatatatct
    18061 tgtgttatcc tataacttgc atccgtaaag tctcttaaag cataactact ttccttacat
    18121 ttagcaactc tcttgtcctt tttacaatct ctaacaacta ctcccatagg acagtattct
    18181 gtaaccatca taggagcata tccatatatt actgcttcaa tttctacatc tgtatatttt
    18241 aaagtttgtt taatttcatt taagtttagt tcttgtgaca aacaaactgt actcgctccc
    18301 tcttctttga agtgatttat actttcacta ttgaatgcat ttaagtagca atctatattt
    18361 aagtcttttt tcttaaagta ttccatagag ccccagttac caatttgaac cttattaatt
    18421 ccttttatat tatccaatat ctcaaattgc ttgtactctt tatttctaac aatccttggt
    18481 gctgaataga ttaatttttt gtcagtgact gctgtattag agtggttctt attcaaagta
    18541 tctattttac ttgtatttga caaagtatct atgttatttg tatttgaacc cattagttct
    18601 ttaggagtac ctaaagacga agaagtatct aaatccatag aaatacttga atctatagaa
    18661 atatttgaat ctatagaaat acttgagacc atttgtaaag cttttgataa tgtatttata
    18721 tcttcataat agattaaatc tacattataa tttaatactg cttctaattg ctccaagttc
    18781 ttaactttaa ctctaatctt aggagctgta tcaagttttt tctgtttatt tactctattt
    18841 ggctggtatt ttaatctatt cttcttaaat tttctatctt ttatctttat tctttcttca
    18901 tctaaaagct caatacaatc ccttctcatc tgatttaaca cacttatagg catacttaca
    18961 ccttcatcca ggtcaatttc tatctctttt aacttatatg gtgtatttcc aagcttttga
    19021 atttgtgttt ctattttttc tttacttaaa gccaccttta tagctttttc aactattttg
    19081 tctccttcta ccaccgattt atttccacaa aaatcactta taaaaagctc tggcttttta
    19141 cctagtttaa tactcacttt tgcacgtata ttagttttta tgaactcttt atcttgctga
    19201 aatgtagctt gtactctatc cattaattcg ctgtcagaag tcttatatat aagctgattt
    19261 ctctttgctt ctccaacaaa atctaattct atcaaatccc ctttatgtgc tattgtagtt
    19321 atctctccat cctttattat tcttccaata gtacctccac ctaagttaat tccatcacct
    19381 ttcttaagcg tattttctag cattactcta agtcttttgt tctttctatt ataatctaaa
    19441 acttttccaa tatatagccc ttgattatta ggtctattag agttcattat atccttacca
    19501 acttcaccta atatatatcc ttttgtaaac tttctattaa atatggtata caaattattt
    19561 attatttcct cagaaatact tatttttttc ttatctacaa attcattaat cgcttctcta
    19621 tatcctgaaa ccacagtagc tacatactct ggctttttca ttctaccttc aatttttaaa
    19681 gataatacac ctgcttcaat tattttatcc aaatcttcta tagtacttaa atctctagga
    19741 ctcaacaaat aatctccatt actttttact acttctcctg tatttatatc tatcaactca
    19801 tacttttgtc tacatggctg tgcacaccta cctctatttc cagacctatt gcctatcata
    19861 ctactcataa ggcattgacc cgagtaacat acacataaag ctccatgtac aaaaacttct
    19921 atatctacag ttgtattttc acaaatccat tttatctcat caacatttaa ttctcttgct
    19981 aaaacaactc tatcaaaccc tacactctga aggtatttta catcttctaa ggaatgtgct
    20041 accatttgag tgcttgcatg tagttcaaag tcaggtaata gtttttttat aagtcttgcc
    20101 attcctatat cctgtactat taaagcatca atatctatat cgtataaaaa ttttacatac
    20161 tctacaaaat cttctatttc atactgtttt atcaatgtat ttacagtaac aaagacttgc
    20221 actcccctta tatgagagta tttaacagcc tcaatcagct catttctatc aaagttattt
    20281 gcagatgctc tagcactaaa gtctttacca cctaaataca cagcatttgc tccgttttga
    20341 actgctgctt ttaatgaatc aaaagagcct actggtgcaa gtaattctat ttttttcata
    20401 tttttctcct ctatttatac ttgttaggtt aatataatta tatctataac ttaaatattt
    20461 gtattaaatt tatatatttc ttactatatt ttaaattctt caatcaattt atatattagt
    20521 tatt
//
LOCUS       DACXMZ010000051        20467 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.051, whole genome shotgun sequence.
ACCESSION   DACXMZ010000051 DACXMZ010000000
VERSION     DACXMZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20467
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.051"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            453..1523
                     /locus_tag="JD016_07315"
     CDS             453..1523
                     /locus_tag="JD016_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4999448.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1507..2376
                     /locus_tag="JD016_07320"
     CDS             1507..2376
                     /locus_tag="JD016_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAU4999449.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2420..3280
                     /locus_tag="JD016_07325"
     CDS             2420..3280
                     /locus_tag="JD016_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAU4999450.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3311..4615
                     /locus_tag="JD016_07330"
     CDS             3311..4615
                     /locus_tag="JD016_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAU4999451.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4678..5940
                     /locus_tag="JD016_07335"
     CDS             4678..5940
                     /locus_tag="JD016_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HAU4999452.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6127..8097
                     /gene="uvrB"
                     /locus_tag="JD016_07340"
     CDS             6127..8097
                     /gene="uvrB"
                     /locus_tag="JD016_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAU4999453.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMIEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8116..10941
                     /gene="uvrA"
                     /locus_tag="JD016_07345"
     CDS             8116..10941
                     /gene="uvrA"
                     /locus_tag="JD016_07345"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAU4999454.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11200..13017
                     /gene="uvrC"
                     /locus_tag="JD016_07350"
     CDS             11200..13017
                     /gene="uvrC"
                     /locus_tag="JD016_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAU4999455.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13017..13946
                     /locus_tag="JD016_07355"
     CDS             13017..13946
                     /locus_tag="JD016_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAU4999456.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14160..15956
                     /locus_tag="JD016_07360"
     CDS             14160..15956
                     /locus_tag="JD016_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HAU4999457.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16096..17877)
                     /locus_tag="JD016_07365"
     CDS             complement(16096..17877)
                     /locus_tag="JD016_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HAU4999458.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17900..19786)
                     /locus_tag="JD016_07370"
     CDS             complement(17900..19786)
                     /locus_tag="JD016_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAU4999459.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19803..20300)
                     /locus_tag="JD016_07375"
     CDS             complement(19803..20300)
                     /locus_tag="JD016_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HAU4999460.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
BASE COUNT         7905 a         2718 c         3501 g         6343 t
ORIGIN      
        1 ttttttttgg gtaaatttat aaaaattaaa aaagttttat tttttatgtt taaaccttgt
       61 aaatttggga cataatataa ttgtaaaact taatatagca aaaataaatt actcaatctc
      121 ccccacttaa agacctctat cccccataga ggtctttttt attttcaaac caaattatat
      181 aaaaaataaa tagttttata tgactcttca taaattaaaa tcacattgtg atagcaaacc
      241 atgaatatga aaaatataga aaattatagc taacataagc tgtatatcat cttaacttca
      301 gtatatttaa caaaaaatgt ttataattta acctttattt taataagttt actatatatt
      361 cactttgtct taatatatcc tttacaaatt aagagtatta ttaatctata agtaaatcgt
      421 agaattaaat taaatatttt gggaggataa aaatggcaga tataagacta gaaaatgtaa
      481 caaaatctta taataaaaaa aatacagtta tagaaaattt aaatcttact atagaagatg
      541 gttcttttac agtattggta ggcccaagtg gatgtggtaa atctactacg ttaagaatga
      601 ttgcaggtct tgaagatata acttcaggta ggttgaagat tgaagaaaat gatgttacaa
      661 aaacggatgc tagtaaaagg gatatagcta tggtatttca aaattatgca ttatatccac
      721 atatgactgt tagagaaaac atagaatttg gtcttaaaaa caaaaaagta gaaaaagaaa
      781 aaagagaaaa acttataaca gaagttattg atgtagtagg attgagagaa tatttaaatg
      841 tgaaaccagg caacttatct ggaggtcaaa gacaaagagt ggcacttgct agagctatgg
      901 ttaaaaatcc aaaggtattt ttaatggatg aaccattgtc aaatttagat gctaaattaa
      961 gaaatcaaat gagaacagaa ttaatacaat tacatcaaaa actaaagtct acatttgttt
     1021 atgtaactca tgaccaggtt gaggcaatgt caatggcaga taagatagtt ataatgaaca
     1081 aaggaaagat aatgcaaata ggttctccaa agaagatata tcaaaatcct aaaaatttat
     1141 ttgtagctca atttataggg agtccttgta tgaacatact agatataaaa aatgatatta
     1201 aatttggatt cagaccagaa aaagcaacac tagagagtat aaacatagat ggaaaaaatg
     1261 atgatgtatt tagtctattt ggaaaagttg taactaaaga agtgttggga aatgaaacta
     1321 tatattgttt atctgttctt aatagagaga taagagtcaa aacggaaaat gatatatttg
     1381 atattggaag aactttaaga ataagtgtat tagaaaaaga tttatattat tttgataaaa
     1441 atggagaaag aatagaagat gtaaaagtat gtaaaagtat gtattctaaa attaaaggag
     1501 atatttatga aaaagcaatt taataaagca tatttatata taacacctgc tattatagga
     1561 actttattgt ttatagtcta tccaataatt aagacgatat ctctaagctt caaatctggt
     1621 agtcttctta gttcaacctt tgaaaatgta ggcttaaaca attatacaga acttttaaag
     1681 aatccagagt ttatacaaac tataacgaac actgcaattt atactttttt tatggtgact
     1741 atatctatat cactagccat agtacttgct gtttggctaa ataaaaattc tataatacac
     1801 aatttaacgc aaagtataat ctttacacca catgtaattt cactagtatc tgtagctgta
     1861 ttgtggcttt ggattatgga gccacaatat ggctttttaa actggatact atcgacatta
     1921 catttaccaa catcaaagtg gctttcaagt gaaagttcag ctttaatgtc actaattcta
     1981 ataggaatat ggaagactct tggatataat atcttaatag tgttatcagg tcttaaatcc
     2041 ataccaaact atatatatga agcatctaaa ttagataatg caaacaaaat aacgaatttc
     2101 tttaaagtga cattgccact attatcacca acaatatttt ttcttatgat aacaacaaca
     2161 actgcatctt ttcaagtatt tgatgagata agtataatga cacaaggtgg gccagtgggt
     2221 tcaactaaca tgttatctta ttatatatat gagtctggat ttatatacta tgacataggg
     2281 caagcgagtg cagcgagtat gtttttgtta gctatagtta tgattgctac atatgctaac
     2341 tttagactct tatcaaagaa agttcattat caataaagaa aaagtagata ttataaggtt
     2401 tatccaaaag gaggagacaa tggatattat acaacctatc aaaaaagaca aagttgatat
     2461 aaaaaaaata gcatcaaaat caatagaaat attaattcta ttacttttat ctgcaatatt
     2521 tatattccct tttatatgga tgctatctac atcctttaaa aatccacaag aaatgatgca
     2581 attaccgccc actattatac caaatgatat agtgttagaa aattatagta cagcatggca
     2641 ttcaggtcca ttttttagat atttgctaaa cagtgtattt gtaacaggaa gtgttttaat
     2701 tgtacaattt ttagttatgg ttccagcagc atatgcattt tctaagatta aatttaaagg
     2761 tgaaaaaatg ctatttgggc ttttgcttgt aggacttatg attccaccac aagtaacttt
     2821 tctaccagtc tatatgatga tgagtaagtt tggatttata aatacttatg ttccacttat
     2881 aattccattt atgacatctt catttgggat atttctgctt agacagaatt ttatgcagat
     2941 ttcagatgaa attattgaag cagctaaact agataatgct tctgacttaa aaataatgtt
     3001 tagaataatg gttcctatgg caaaaccagc agtaataaca tttatattat ttaattttat
     3061 ataccattgg aataattatt tctggccctt agtaatgaca aatacagatg caataagaac
     3121 tttaccaata ggagtagcgc tccttaaatc ttcagaagga attactgcat ggaatgtaat
     3181 aatggcagga aatataattc taattttacc aataattctt gtttatatat ttgcgaataa
     3241 aaaagtaaaa gaagctttta tgtattcagg aataaaataa aaaattaaag actaaatttg
     3301 gaggatgaaa atgaagctta aaagaaaatt agcaacagga ttagcaataa cattattatt
     3361 aggttcttta gttgggtgtt caaattctaa tgatgcaaat gcatctaaag gagaaaagac
     3421 tgatagcaat attacgaaga tagatttctg ggctccttta ggtggaacta atggagaaac
     3481 tgctcaaaaa atggttgacc aatttaatag tgaacataag gatgtacaag taaatatgtt
     3541 aaaacagaaa gattactatg aaaatgcaac aaaactacag gcagccttaa catctaaaga
     3601 ccaaccagat gtaacattat tagaaataac tcaaacaggt acattcgcat cagcaggtgc
     3661 tttagtagat atgagtaaat attttgataa aacatatcaa gaaagattct tttctggtct
     3721 tttaacaaat tcatattatg aggataagtt tgttggtatg ccatttaata gaagtacacc
     3781 aatattatac ataaataaag atatggcaac taaagcaggt cttgaccctt caggtccaaa
     3841 atcatgggaa gagcttaagg attatgctag taaaatgaca aataaatcag aagatactta
     3901 tggttttgaa acgccaatag atatttggtt ctatgaagct atggtaatgc aaagtggagg
     3961 agaaataact aatggtaaaa aagtagcatt taataatgag gcaggacaag caccagttaa
     4021 gttctggcaa gatatgatga aagctggaat aatgaaaatg cctcctggag aagattataa
     4081 tgcctgggac gtagcaaaac aagattttgt aaatggtaaa gtaggtatga tatttacatc
     4141 aacagcagat ttagcaggac ttatgcaaca aacagaaggt aagtttgaaa tatcaacagc
     4201 attcttacca aaaaatcaaa aatacgcaac tcctacaggt ggagctaatc tagttatgct
     4261 tgaaggtgga acagataaag aaaaagaagc aagtgcagaa tttatagaat ggatgacaca
     4321 aacagataaa atcgttcagt ttagttcatc aacaggatac ttaccaacaa ctgaagatgc
     4381 aacaaactca gaaaagttac aaacattata taaagaaaaa cctcaatata aggttgcaac
     4441 tgaccaatta caatatgctg ttgcacttcc aatgttaaat ggatacaagg aagcaactga
     4501 taagctaatg gatgagatta aaaaggggct tactgattta aatgcaaatc caaaagatgt
     4561 agtaagcaaa ggaacttctg caatgcaagc agtaatagat aaaacaaata aataattata
     4621 tagacttgta agttgattaa aagtattcaa taatatataa atgaagaggt aatgtaaatg
     4681 acaaaggtta tagtattatc agtagattct ttatttgaaa aagacttgga atttgtaaaa
     4741 aatctaccaa actttaaatc tatactagaa aattgctcca tagtaaaaaa tataaattgt
     4801 gtttatccca ctctgacata tccgtgtcat actagtatgg ttacaggagt atatcctaaa
     4861 aaacatggta tatatcataa tgaaaaattt gacccaaata aagaaaataa agactggtac
     4921 tggtattcaa aggacataaa agcaaaaact attatagatg tagcaaaaga aaataatcta
     4981 actacctcta gtgtattatg gcctgtaatg ggagcaaatc caaatataga ttataatatt
     5041 gcagaaatat gggctcctag tagagaagat gacccaagag aaacttttga acgaagttcc
     5101 agtaaggtta taatggatgg tatttacaat agacattgtc attatatcga ttggaaatta
     5161 gaacccaata tggatttatt tggagtaaac tgttcagtag atataataaa ggaatataaa
     5221 ccagatttaa tgctaataca tttagctaca ttggaccata cacgacacaa ttatggctta
     5281 tttaatgacg aagtagataa agcattaaaa atgaatgata aatggttagg agatattatt
     5341 caggctacaa aggatgcagg tacatacaaa gatacaaatt ttataatact tggggaccat
     5401 ggtcatctta gagttgataa agttataaat ccaaatgtct tgcttaaatc aaatggattt
     5461 ataaaagttg aaaatggaga agttaaggat tttgatgcct atgtaaattc ttctggaata
     5521 tcagctcaaa taatagtaaa taacttagat aggttaacag aattaagaga actgcttgaa
     5581 gaatttaaag aagaattatt catagagaat atttttacca aagaggaagc atctaacctt
     5641 ggtcttgaag gtgattttga aatggtaatt gaaggtcttg aaggaatttc atttggaaat
     5701 gatttcgaag gaagtattat aaaagattca gatataaaag attacaagtt tgctgttgcg
     5761 acacatggtc atttacctac taaaggtaat agacctccat ttatagcttt tggaccaaat
     5821 attaaaggtg gtgtggttat agaagaagga gacctgagag cacatgcagc aactatatta
     5881 aaaatgttta atttaaaatt agatggagtt gaaaaagaag catttgattt tattatgtaa
     5941 gttgaataaa gtatttattt tccatgaatt atagacttct attgcaaaga tagaagtctt
     6001 tttttatgtt ttagatttaa gtaaaattta aatgggtaaa atatttaaaa taacttaaac
     6061 aattttataa aggtatgata taataataca tgtcaggaac taatgttcga aaaaaggagt
     6121 gatactatgg attttaaaat aaaatcagac ttcaaaccta caggtgacca accagaagct
     6181 attaaatcaa tagtggattc tataaataga aatgagaaat tttcgacatt attaggtgta
     6241 acgggttcag gaaaaacatt cacaatggca aatatcattc aacaagttaa gaaacctacc
     6301 ctaataatgg ctcataataa aactctagca gcacaacttt acagtgagtt taaagaattt
     6361 ttcccagaca atgcagttga atactttgta agttactatg attattatca accagaagca
     6421 tatgtagctc acagtgacac ttatatagaa aaagatgcaa gcataaatga tgaaatagat
     6481 aaacttcgac attctgctac agcatctatt ttagagagaa gagatactat aataatatca
     6541 tctgtatctt gtatatatgg acttggtgac ccaaaagact ataaagaact tatgttatct
     6601 ataagaccag gtatgcaaag agacagagat gatgtaataa aaaggttaat agagatacaa
     6661 tacgaaagaa atgacataaa ctttacacga ggtaccttta gagttagagg agatatacta
     6721 gaaatattcc cagctagtaa tgatgaaaaa gcaataagaa tagaattttt tggtgatgag
     6781 gtagatagaa taacagaaat tgattatgta acagggaaga ttgttggtac tagaaatcat
     6841 gttgttatat tcccagcatc tcactatgta acaacgccgg aaagaattga aaaggcaatt
     6901 gtagaaatag aaaacgagct tcaagaacaa attaaatttt tcaaggaaaa tgataggcta
     6961 ttagaagcac aaagaattga acaaagaact aagtatgata tagaaatgtt aaaggaaata
     7021 ggattttgtc aggggataga gaactattct agacatatta caggaaggtc agaaggagaa
     7081 agaccatata ctttaatgga tttcttccca gatgattatt tgataattgt agatgaggca
     7141 catgtcacta taccacaagt aagaggtatg tatgcaggag acaggtctag aaaaacatca
     7201 cttatagaga atggttttag attaccttct gcacttgaca atagaccatt aaattttcaa
     7261 gaatttgaag gaaatataaa tcaaatgttg tttgtaagtg ctacaccagg accttatgaa
     7321 attcaacact cagagacaat agcagaacag ataataagac caacaggcct tttagaccca
     7381 attgtagaag taagacctat aaataatcaa atagatgatt tggtaggaga aataactaaa
     7441 actatagaaa aaaatgaaag agtgcttata accactttaa ctaaaaaaat gagtgaggac
     7501 ttgactaact atcttaaaga aatagggata aaagttaaat atttacactc agatatagtt
     7561 acattagaaa gaacagaaat aataagagat ttaagattag ggaagtttga tgtccttgta
     7621 ggaataaact tacttagaga aggattggat ataccagaag tttcacttat agcaatttta
     7681 gatgcagaca aagaaggttt cttacgttcc gaaacagctt taatacagac tattggacgt
     7741 gcagctagaa atgaaaatgg aagagtaatt atgtatgcag acaggataac tgactcaatg
     7801 caaaatgcta ttgatgaaac aaagagaaga agagatatac aaaatttata taatgaagag
     7861 cacaatatca ttcctaaaac tatacagaaa aatataagag atagtataga agctactaag
     7921 gttgcagagg aagaagttgt atatggaata tctgatacag atgataaaga cgaaataaga
     7981 gctaatatag ataaattaaa atcagagatg atagaagctg ctcaaaactt acaatttgaa
     8041 agagcagcag aacttagaga taaagtgaaa caattagaag aaaaattgga aaaatagaaa
     8101 ggtaaaaaca acattatgga agataaaatt ataataagag gagcaaaaga acataattta
     8161 aaaaatatag atttagaatt accaagagat aaatttattg tttttacagg tttgtcaggg
     8221 tctgggaagt cttctttggc atttgataca atatatgcag aagggcagag aagatatgta
     8281 gaaagtctat ctgcttatgc aagacaattt ttaggtcaga tggaaaagcc aaatgtagaa
     8341 tatatagagg gtttatcacc agctatctca attgaccaga agactacctc aaaaaaccca
     8401 cgttctactg ttggaacagt aacagaaata tatgattatt tgagactatt atttgcaaga
     8461 gtaggagatg tgtattgccc tacatgtggt gaaaaaataa gtcagatgac tattcaagaa
     8521 atagttgata aaatgctaga ttttccagat agaacgaaat tacaaattct ttcaccaatt
     8581 gttagaggac aaaaaggtac acataaaaaa gtctttgata atatcaaaaa agaaggattt
     8641 gtaagagtta gagttaatgg tgaaaactat gaagttagtg atgatataat cttaaataaa
     8701 aatcaaaaac ataatataga agttgtagtg gacagaatag taattaaaga tggaatagaa
     8761 tcaagactag cagattcaat tgaaactgca gttaagctat ctgatggtct tgtaatagcc
     8821 caaataatag atggagaaga aattcttttt tcaactaaat ttgcatgccc agaacatgga
     8881 attggcatag aagaattatc tccaagaatg ttttcattta atgctccctt tggtgcttgt
     8941 gatgtttgta atggtcttgg ggaaagtaaa gaagttgacc cagaattagt agtaccaaac
     9001 aaagatttat caataaaaca aggtgcagtg gcagcatggg gttcaacagg tgttaatgat
     9061 gacacttatt acagtaaaat gataaaaagt ttagcagagc attttaatgt atctttgaca
     9121 acaccgttta aagatttacc agatgatttt gtggaggaat tactttatgg taaagataat
     9181 ataattgtag agtttactta tgagtcaaaa tttggaggaa ctagaaatta taaatcatac
     9241 tttgaaggtg taatagtaaa cttagagaga agatatagag aaactaattc agattacatg
     9301 agagataaga ttgaagaata catggcagag agaccatgtc caaagtgtaa agggatgaga
     9361 cttaaaaagg aagttttatc tgttttagtt gatggaaaaa atataatgga agttacaaac
     9421 ttatctgtaa atgaattgat tgactttatg gaaaatataa atcttacaga aaaacagaga
     9481 tttatagctc atgagattat aaaagagata aaaggaagag ctatgttctt aagagatgta
     9541 ggtcttgatt atttaaattt atcaagaaaa gctggaactt tatcaggagg agaagcgcaa
     9601 cgtataagat tagctactca aataggttct gcattggttg gagttttata tgtacttgat
     9661 gagccaagta tagggcttca ccaaagagat aatgataggc ttataactac tcttagacat
     9721 ctaacagatt taggaaatac cttaatcgtt gtagagcatg atgaagacac aatgagagaa
     9781 gctgattatg ttatagacat tggtccaggt gcaggtatac atggtggtga aatagttgca
     9841 cagggtactt tagatgaaat aattgaaaat gagaactcaa taacgggtca atatttaagt
     9901 ggtaaaaaac aaatattgct tcctgagact actagagaag gaaatggaag ttttatagaa
     9961 atagttaaag catcagaaaa taatctgaaa aatattgatg taaagttccc attaggtaag
    10021 tttacatgca taacaggtgt ttcaggttct ggaaaaagta cattaataaa cgatatactt
    10081 tacaaaggtg ttgcaagtaa agtgaataga cttaagcaaa gagctggtaa gcataaggag
    10141 ataaaaggaa ttgaaagtat agataaagtt ataaatatag accaaagtcc tattggaaga
    10201 acacctcgtt caaatcctgc tacttataca ggtgtatttg attttataag agatattttt
    10261 gcatctacta atgaagctaa agcaagaggt tataaaaaag gtagatttag ttttaatgta
    10321 aaaggtggta gatgtgaagc ttgtaagggt gatggaataa taaaaataga aatgcatttc
    10381 ttaccagatg tatatgttcc ttgtgaaatt tgtaaaggtg aaagatacaa tagagaaaca
    10441 ttgcaagtaa aatacaaaga taaaactata tctgatatat tagatatgaa tgtggaagaa
    10501 gctgttgagt ttttcgaaaa tataccaaat ataaaaagaa aattagaaac tttaatggat
    10561 gtaggacttt catatattaa attaggacag ccttcaactc aattatcagg aggagaagca
    10621 caacgtataa aattagctgc tgaacttagt aaaagaccaa caggaaagac tttgtatata
    10681 ttagatgaac ctactactgg acttcatatg gcagatgtag acaagctaat ccaagtatta
    10741 caaagactag cagatacagg aaatacaatt atagtcatag aacataattt agatgttata
    10801 aagacttgtg attatgtaat agacttagga ccagaaggcg gagataaggg tggaactata
    10861 gttgcaactg gaacaccaaa agaagtatct aaagttgatg gttcttatac aggtcaattc
    10921 ttaaagaaat atttttctta agttatataa attgtgaaaa attttagagc agatatacga
    10981 taaactgttt tgttataagt aaattagtca aatataagtt gtaaatagta atacaaagta
    11041 aagtccatat tactcttcat ggttaaaatt gttttcaaat caattataga agggaatatg
    11101 ggctttaact tgtctaaagc actggaaatt gtataacttt ttaaaattca tattttaggg
    11161 tataatttta gtataaaatt taatgttttg gagggcgaga tgtttgatat acaagagcat
    11221 ttaaaaaaat taccttctga gcccggagta tatttaatga aagacaaata cgaccatata
    11281 atatatgtgg gaaaagccat atctctaaaa aatagggtaa ggcagtattt tcaatcttca
    11341 aaaaaccata catctaaagt aaaatctatg gttaagaata tatataagtt tgagtatatt
    11401 ataacggatt cagagttaga ggcattaata cttgaatgta atttaataaa aagatacaga
    11461 ccaaagtata atgtagtgct tagagatgat aaaacttatc catatataaa agtaactaca
    11521 aatgaagatt atccaagaat cttaaaagtt agaagagttc ttaaagataa ggcaaagtat
    11581 tttgggccct ataccaatat aactgctgtg aatgatacct tggagcttat aagtagtacg
    11641 tatccaataa gaagctgtaa aatagatata gataaagcaa taaagaataa gacaagacct
    11701 tgtttaaatc tacacataaa taaatgttta ggaccttgta caggaaatgt ttctaaagaa
    11761 gagtatggaa aaatgataga agagattata atgtgtttat ctggtaaaga agaaaaattg
    11821 atggaacttt taaaggaaaa aatgaatgag agttctatga actttagatt tgaagaggct
    11881 gctgtatata gggataagat aaagagcctt gaagagatga tacagaaaca aaaaatagat
    11941 gctactgtaa gtgacttaaa ccaagatgtg gttgcaatgg caagagctca taatgaagct
    12001 tgtgtccagg tattttttat aagaaatggt aagattgtag gtagggaaca ttttatatta
    12061 gaaggtgtaa tggatagtcc aagagcttct atattaagtt catttgtaaa acaattttat
    12121 aatgaacaag agtatatacc taaagaatta attatagaag atgagataga agatagttct
    12181 atattagaag aatggctgtc atctaaaaag gggcaaaaag taacaataag agttcctcaa
    12241 aagggagaga agaaaagctt agttgaaatg gttaggaaaa atgctgtaga atatcttgaa
    12301 aaattttctg atatgaataa aagaaaatat gaaaaaagta ttggagcatt ggaagaatta
    12361 aagcaaatat taaatctaga aaagttgcca ataagaatag aggcttatga tatatcaaat
    12421 atacaaggtg tagattcgat tggttctatg gtagtgtata cgaatgctaa aaaagataag
    12481 aaggaatata ggagatataa aataaagaca gttattggac ctaatgatta tgattctatg
    12541 gctgaaattg tagatagaag attaaaacat ggaaatttac ctgacttgat attgttggat
    12601 ggtggaaaag gacaagttag tgcagtaaaa aaagtactag aactcaatga tgtagatata
    12661 cctctttggg gaatgtacaa agatgataag catagaacta aaggattgat atgcaaagaa
    12721 aaagaaatag aactagataa aacaactaat ttatatagat ttattgctag tatacaagaa
    12781 gaagttcata attatgcaat aacatatcat agaagtctaa gaaataaagc tttgacaaaa
    12841 tctatcttag atgatataca aggaataggt gaaaaaagaa agaaatcatt attaaatcac
    12901 tttaaagatg tagatgctat aaaaaaagct actatggaag aacttttaga agtggatgga
    12961 atgaataaaa gcattgcaga taatgtttat aattttttta gaaaggaaga aaattaatgg
    13021 aaactagcaa taaagtatca ataagagaga taataaaaga gttgaagtta aaagtagtat
    13081 acatgcctga tgatgtagat tattatgtct actctcaaga tataaataga ccaggattac
    13141 agtttgcagg atattttgat tattttgcat atgaaagaat acaaatagtt ggaaagacag
    13201 aatataattt ttttggatta atggatagtc atattcgaga agaagtacta gatagatttt
    13261 tctcatatga aataccagcg ttaataattt ctagagactt ggaaatcaag ccagatgtaa
    13321 ttgaaaaggc taaaaaatat aaaagaatac ttttgagcac aaatagaaat accactagac
    13381 ttataaatag actgtcaaac tatttggata ataagttagc accacacaca acaattcacg
    13441 gggtgctagt agatatttat ggtataggtg tactgataaa aggtgaaagt agtataggaa
    13501 agtcggaaac agcattagaa ctgattcaaa ggggtaatag attagttgca gatgatgctg
    13561 tagaaataag aaaattagat gaaagtatgc ttgtgggtca gtcacctgaa ctaataagac
    13621 attttcttga gataagagga ataggaataa tagatgttag aagtttatat ggagtagggg
    13681 caatcaaaaa ttctaaaatg atagatttag tagttcattt agaagcttgg gatgaaacta
    13741 agtattatga tagactggga ctggataaag aatatgaaga aatacttggt gttaatgtag
    13801 agaaattagt tgtacctgtt aaacctggaa gaaatactgc tatgatatta gaagtagcag
    13861 caatgaattt tagacaaaga ggaatgggat acgatgcagc tcaagaattt actaaaaaat
    13921 tatcaaaatt aatcgatact aagtagtact gaataatagt gaactacaca tcatttaatt
    13981 aaatattgat taggattatg tcctacacct tataaattaa aatcaagttg tatatatata
    14041 atgtttatta ataagatata ttatataagg ataatttaaa gtacagtgtt agattataat
    14101 aatactagat taattttata tttgagtttc attatataac aagaaagttg gaatttttta
    14161 tgggaataga atattttagt aaacttttag aaagtagtac tgaaaaagaa ttgctcttaa
    14221 gtaaaaagtc aaataacata agtacagcaa gattaatatc ttttttgata gtgatagcag
    14281 ggcttgctat tggattttac aataaaaata tagtaggtat ttttgttgga gttttatcaa
    14341 taataatatt tatagcactc ttagttattc atagtaaagt aaaagaagaa gaaacatatt
    14401 ttaaaagcaa gagcgaagta ttaaataagt atattaagag atttggagat ggttggaagg
    14461 aatttaaaat agatggaaaa gaatacttaa aagatgaaaa ctcgcaagca aaagatttag
    14521 acttatttgg aagagcatca ttgtatcaat atatctgtgt agctaataca tcttatggga
    14581 aaaatttttt agccaaatat ttgtggaatg aaaatccaga tgagaatgta atcttagaaa
    14641 gacagagagc tataaaagaa cttttatcaa aagatgattt ttctattcat attcaaacac
    14701 tcagtaatat aattggtaaa gagcaaaaaa acaattcaga tagtagtata gaatcgttta
    14761 tagaatatgg agaaaataag aatgtgtata taccaaaatg gatgcatatt tttacttggg
    14821 gtttaccaac tgcaacaatc ttatctttta tattttgtat gttaggattt ttacctgttt
    14881 tacctgtttt tttacttttt ataatccagt tagggtttag tggttttgga tatccaagac
    14941 ttatgcaaac tcttgctcca ttattttcat ttagtagaag tatacaagta tatgaaaaaa
    15001 tgtttgaggt tttagaaaaa gagacgtttg agagtgcata tttgaaagaa ttacaagata
    15061 aactttctaa gggaagtgga gtttcaagag gtgttaaaca attaaattca ataggaaatg
    15121 ctgtaaacct aaggtataat cagatactat atataattgc ctgtggtgtt ttgatgtgga
    15181 actatcattg tgcagaagct ttggagcgat ggaaaggtat atatggaaat caaattagag
    15241 attggtttga atctattgga gaatttgaag ctctaataag tttaacagtt gtttctcatg
    15301 taaaggaaaa tacatgtttt ccagtaataa aatatgaaga tactccaaga ttgaaagttg
    15361 aagaggtata ccacccatta atagcagaaa aaagtgtcat agcaaattcg atacaattaa
    15421 attctcagac ttgtattatc acaggttcaa atatgtctgg aaagacaaca tttttaagaa
    15481 gtatcggtgt aaacttagtg ctggcatatg caggtgctcc agtatgcgct aagagttttg
    15541 atgcgacctg tatggctata tttacttcta tgagaataca agatgatgta agtcaaggaa
    15601 tatcaacttt ctatgctgag attcttagaa taaaatcaat gattcaatat agtttaaaag
    15661 aactgccaat gttggttcta gtagatgaaa tttttaaggg aactaactct gctgatagaa
    15721 ttattggagc aagtgaggca gtaaagaaat tatcaaaacc ctgggttata agtatggtga
    15781 caacacatga ctttgagcta tgtgaccttt caggtagcgg agatgttgaa gttgtaaact
    15841 atcatttctc agagtactat gtagacgata aaattcattt tgactataag ataaaagatg
    15901 gaagatgtaa aactacaaat gcaaaacaac ttatgagaat ggcaggtatt ctttaattaa
    15961 tagataggtt aattaataaa taagtctatt tagaaatata atttaatttg tgaaattaat
    16021 aaggattgtc ttaaattacg taaaagtatt ttaagacaat ccttttttaa gaataaattt
    16081 tatactttgt tatgactact tagcttcttc acaagcacat tcttgtggac actctccata
    16141 gacacaactc ttatcaaagt aatgagtatg aaggtattta tgagctacat cactatttgg
    16201 ttctccaaag aagtctttat aaatagcttg taaatcagga ttctcatgag atttacgaat
    16261 agccttattt ctatctattt catataacgc atttgcacgt ctatcaacta tttcagtatt
    16321 accatggtga taaggttgac cagcacctcc aacacatcca ccaggacatg ccataacttc
    16381 tatgatatgg tatttacatt ctccacttct gaccttatcc ataatctttc tagcgttttt
    16441 aagtgtattt acaatacaca cattaactgt agttccatca acatctatac ttgcttccct
    16501 aattccttta agacctctaa cattagtgaa gtttacatta tctaaagtct tattagttat
    16561 cttctcatat gaagtacgta gggcagcttc taaaactcca ccacttgctc caaatataga
    16621 tgcagcacct gtagattttc ctagaggatt atcaaaatct tggtcttgaa gattaggtaa
    16681 atcgatagca gcttctttta tcatcttagc taactctcta gtagttatag aaaggtcaac
    16741 atctagtatt ccagattcac ttaattcttc tcttgaagct tcatattttt tagctacaca
    16801 aggcattaca gatacaacat aaagttcttc aggattgatt cctaatattt taggtgcata
    16861 gtagttttta gctatagaac caaacatacc ttgaggagac ttacagctag atgctaagtt
    16921 taacttgtct ggataattgt gctctaagaa gtttacccat gcaggacaac aacttgttaa
    16981 tataggaaga ttttctcctt tttggattct ttctataaat tcagtagctt cttccattat
    17041 agtaaaatct gcaccaaagt ttgtatcaaa tacatgttca aatccaagtg cttttaaagc
    17101 agctaccatt tttcctgtag agattgaccc tggttctaat ccaaactctt caccaatagc
    17161 aactcttaca gcaggagcaa cttgcactac tatagtcttt tcttttttat ttaaaacatc
    17221 ccaaagttta ggtacattat ctacttctgt taatgctcca gttggacaga cagatataca
    17281 ttgaccacag aatgtacact ctgtttcaac catatcagag ttaaagaatg tactaactaa
    17341 agtattaaat ccacgattaa ctccagataa tgctcctact gtttgtatgt cattacatac
    17401 agtctcacat cttctacata atatacactt agaatggtct ttaactaaag atttagtaga
    17461 agtatcttta cctgcaaaag atttagaacc ttgatatctt atttttctta ctcctaaatc
    17521 agcagctata gtctgtaatt cacaatctcc atttttttca cagacaaaac aatcttgtgg
    17581 atggtctgat aacaataatt caacaatagt tctacgagca tttaatgctt tagcactatt
    17641 agtctgaact ttcatacctt cctttataac tgttccacaa gctggaacta atcctctctc
    17701 tgtctcaacc atacaaacac gacaagaagc acaagtgtca agcttatcta tttcattcat
    17761 atgtaaatga caaaggttgt gtatttttat atttattaat ttcgcagcat ctaagataga
    17821 agttccacta ggtgctgaaa cgtgtttgcc attaatagtt aaattaacta aactcattgt
    17881 tattcctcct ccaaataagt tatttcttga ttatagcgtt aaatgtacaa ttatctatac
    17941 aagcaccaca ttttagacac ttagaagtat ctattgtatg ttgttcctta acagaacctg
    18001 ttatacaacc agcaggacac actctagaac attttgtaca acccttacag tcatctgtta
    18061 taaagtaaga aagtaagttc tgacatttac cagcaggaca tttcttatct acaacatgag
    18121 ctatatactc atctttaaaa tattgaagag tacttaaaac agggtttgga gcagctttac
    18181 caagaccaca taatgaagca gtttgtatag tttcagcaag gttttctagg tcatctaaat
    18241 cttgtagagt accattacct tctgtaattt tagttaatat ttctaataat cttgtagtac
    18301 ctatacgaca aggtgtacat ttaccacaag attcttcaac agtaaactca aggaagaact
    18361 tggctatatc taccatacag tcagattcat caagaacaag cataccacca gagcccatca
    18421 ttgaaccgat agaacttaat gaaccaaagt ctataggagt gtctaaatgt tcagttggta
    18481 tacatccacc agatggtcca cctgtttgaa cagccttaaa tttcttacct tcagggattc
    18541 ccccaccaat ttcaaataca atatctctta aagtagtacc cattggaact tcaacaagtc
    18601 ctacattttc tacttttcca ccaagagcaa agactttagt accactagaa tcttcagtac
    18661 ctaagttggc aaaccaatct ccacccttaa gtataattgc aggtatgtta gcaaaagtct
    18721 caacgttatt taagcaagta ggagatttcc atagaccact cttagaagaa ctataagttt
    18781 tcattctagg ttcaccacgt ctaccttcaa ttgaatgcat tagcgctgta ccttcaccac
    18841 aaacgaaagc acctgcacca tattttaatt ctaatttaaa gttaaatcca gtgcctaaga
    18901 tattttcgcc aagtaaaccg tatttttcag cttgagctat ggctactttt aatctttcta
    18961 tagattttgg atattctgct cttatgtata tgtatcctgt atcagaacct atagcgtatc
    19021 cacatattgc catagcttct agtacactat gagggtcacc ttcaagtaca gatctatcca
    19081 taaatgcacc tgggtcacct tcatcagcat tacatacaac aaacttttta tctgttggac
    19141 cagctggatt ttttgaagca gcttcccatt tagagccagt agggaaacct gcaccacctc
    19201 taccacgaag gccagaagtt tttacctctg caataacttc ttgaggagta agagaagtta
    19261 aacatttgcc tagagctaaa taaccatcat tggcaatata ttcttctata ctatctgggt
    19321 ctataagacc acaatttttt aaagctatac gtaactgttt tttgtagaaa gacatttctt
    19381 cttgttcttc aactttttta cttaaagatt cttcctcata taataattcc tcgacaactg
    19441 tatttctaat taagtggctt tgaactatct tttcagcatc agatggctca acttttacgt
    19501 agaaaacgtt atcaggataa acttttacaa taggaccttg agcacagaaa ccaaaacaac
    19561 ctgtcaaacg aacactaact ttatcttgga ttcctgattt ctttatttca ttttctaatt
    19621 cactaactat ttctagactg tttgaagatg tacatccagt atcacaacaa acaagtaatt
    19681 ctcttcttaa tacattttcc ttagaataat tttttcttaa gctaagatta ggtttcagct
    19741 catcagctaa aacttttaat tcatcaaatg aatttacttt acgcattatt tattcacccc
    19801 tcttaacaat tcaattctaa ttctctataa gtatctaata tttcgcttac ttggtcaggt
    19861 ttcactttac cataaacttt accatttaca gtaacaactg gagctaaacc acatgccccc
    19921 aaacatctta aaccttctaa agaaaattta aaatcactag tagtttctcc aacttttatg
    19981 ccaagctgtt tttcaaaagc acttaagatt ttgtcagcac cttttacaaa acatacagtt
    20041 cctagacaga cactgatttt atacttacct acaggttctg tagtaaaata agaatagaat
    20101 gtaacaaccc catatacctt tgcaggagct actccaagtt ttctagcaac atgaagttga
    20161 acttcttttg gaagatatcc aaatatacct tgggcttcat gtagaacttg tattaacgca
    20221 ccttcctttg ttgcaagaga gtcaataaat atatctagtt cgtcaaataa ttgcttgttg
    20281 tgtgaaataa aatcgcacat aacaaatccc ccttttcttt ttaaagttat tatttttcat
    20341 aaaataatta accatgagtt gaccccaaca ttatgtgggt gaacaaaacc aaaacaataa
    20401 agaaagaata atatacaaca atttctttat ttataaaaac aatgcaaaaa agttacctat
    20461 taaaaat
//
LOCUS       DACXMZ010000052        20040 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.052, whole genome shotgun sequence.
ACCESSION   DACXMZ010000052 DACXMZ010000000
VERSION     DACXMZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.052"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            52..1893
                     /locus_tag="JD016_07380"
     CDS             52..1893
                     /locus_tag="JD016_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAU4999461.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSSRESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            2198..2905
                     /locus_tag="JD016_07385"
     CDS             2198..2905
                     /locus_tag="JD016_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4999462.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            2935..4209
                     /locus_tag="JD016_07390"
     CDS             2935..4209
                     /locus_tag="JD016_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU4999463.1"
                     /translation="MKDDYIMRRVIVYFVLSVLIITLVFILFEYSRLHKLYEHQIKNT
                     QNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLENKMSNDKNFSLYL
                     KSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQENYLKDTTESEET
                     GYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSLVTDISHQLKTPL
                     ASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISRLEISMINIKKED
                     NDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESIFNVLDNAVKYTQ
                     VNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADEIESVEGSGVGLY
                     LSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            4326..5009
                     /locus_tag="JD016_07395"
     CDS             4326..5009
                     /locus_tag="JD016_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4999464.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            5002..7389
                     /locus_tag="JD016_07400"
     CDS             5002..7389
                     /locus_tag="JD016_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAU4999465.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMNNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            7669..8811
                     /locus_tag="JD016_07405"
     CDS             7669..8811
                     /locus_tag="JD016_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HAU4999466.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKVPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            8830..9354
                     /locus_tag="JD016_07410"
     CDS             8830..9354
                     /locus_tag="JD016_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAU4999467.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            9454..10272
                     /locus_tag="JD016_07415"
     CDS             9454..10272
                     /locus_tag="JD016_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAU4999468.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDFMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGVSFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLSPTQSVEKVVLCLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            10350..10661
                     /locus_tag="JD016_07420"
     CDS             10350..10661
                     /locus_tag="JD016_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999469.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(10838..11521)
                     /locus_tag="JD016_07425"
     CDS             complement(10838..11521)
                     /locus_tag="JD016_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999470.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(11614..12255)
                     /locus_tag="JD016_07430"
     CDS             complement(11614..12255)
                     /locus_tag="JD016_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAU4999471.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYDVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMQVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(12429..14432)
                     /locus_tag="JD016_07435"
     CDS             complement(12429..14432)
                     /locus_tag="JD016_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan-branching enzyme"
                     /protein_id="HAU4999472.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLSFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(14585..15223)
                     /gene="recX"
                     /locus_tag="JD016_07440"
     CDS             complement(14585..15223)
                     /gene="recX"
                     /locus_tag="JD016_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAU4999473.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            15499..16188
                     /locus_tag="JD016_07445"
     CDS             15499..16188
                     /locus_tag="JD016_07445"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAU4999474.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            16189..16761
                     /locus_tag="JD016_07450"
     CDS             16189..16761
                     /locus_tag="JD016_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAU4999475.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            16878..17228
                     /gene="rsfS"
                     /locus_tag="JD016_07455"
     CDS             16878..17228
                     /gene="rsfS"
                     /locus_tag="JD016_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAU4999476.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            17582..20002
                     /locus_tag="JD016_07460"
     CDS             17582..20002
                     /locus_tag="JD016_07460"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAU4999477.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDALEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
BASE COUNT         7842 a         2255 c         3355 g         6588 t
ORIGIN      
        1 aatataaatt ttatattaaa gctaaaacta atttgaggag gctaatttat attgaagatt
       61 agaaggtatt taattttggt aattataatt ctaattacaa caagtcagag ttttatttct
      121 tatgggctag agcgatttga agaaataacc atttttcaca ctaatgatat acatggaaga
      181 tatgctgaag gtgatgacca tatacaaata ggaaatctag ccacattgaa aaaagaaact
      241 ccaaactcaa ttttagtaga tgcaggagat tgtttgcatg gacttccaat agtaaatatg
      301 gatagaggga aaagtgcaat tgaattaatt aaatgtgctg gatatgacta tattactcct
      361 ggaaatcatg actttaatta tggaaaagat agactgtttg agttatctaa aagtgctaat
      421 ttaggagaga atactttaaa atttttatca tcaaatattt ttgaaaatgg aaaaaaagtt
      481 tttgatagta atgatataaa agaaatagat ggaataaaaa taggattttt tggactctca
      541 acccaggaaa caaagagcaa aactggattt aaaaatgtgg aaggattaga gtttaaaaat
      601 cctatagaaa gtgcaacaga acaagtaaat gaattaaaag gaaagggtgc caatgtaata
      661 gttgcaatat gtcatattgg tacaaatagt ttaagtaagc caaatagtat agatatagca
      721 aatgaggttc aaggaattga tttgattatt gatggacaca gccacactaa gtttgaaaat
      781 ggtaaacaag tgggtgatac attgattgta agtgcaggac aatatttaga aaatattggc
      841 gagataaaac taactttgga tactaatgat gtagaaaata taaaaataga aaataaatca
      901 gttagattaa ttggaaaaga agaagcatta aagtataagc ttgactccgc tgtaagtaaa
      961 aaggttgatg aaataaaaga acaacaagaa aaaatattag ataaagttat aggggtaact
     1021 acaaatactc tagatggttc atatgaaaat gtaaggacaa aagaaactaa tttgggtaat
     1081 ttaataagtg acattttact agataaaact aaggctgata tttctttatt taatggaggg
     1141 aatattcgag atacgataga aaaaggcaat ataacaagaa gagatattgt agatgtattt
     1201 ccttttagca atactatagt taccaaagaa cttacaggag cacagattaa agatgtattg
     1261 gagcatggag taaaacttta tccagaaaaa agttcagcct ttttgcaagt gggaggaatt
     1321 tcatattact ttaatcctaa acaaaaagaa ggcgaaaaaa taacaaatat tcaaaaagaa
     1381 ggaaagccat tagatttaaa taaaaagtat gtagttgcaa cgaatgatta tatagcatca
     1441 ggtggagatg aatttccttg tttttcaaaa gagccagtac taaaggaatt tggaaattta
     1501 gagagtgcag tcattgaata tatagagtac aaaaaagaaa tatcaaaaaa tgtagatgga
     1561 agaattggta ttaaatctaa agatgagtta ataagcaaga atcatgagag tgatttgata
     1621 ttaagtgaaa atgaaaaaaa tatcaatgat aatgaagaga atctatctaa taatataaaa
     1681 agctctgata gtgataaaaa taaaagctta gataaaacaa gttctagaga aagttctaat
     1741 ctaggaatat ctaaaagaga agctaaagaa aaatctccta aaacaggaga tttaggtttt
     1801 tcaaatagta taattatatt tatagtttca agtacattga tatgtttatt gaattttaat
     1861 caaaaagagt taaaggataa aaagtcaaaa taaaaattaa tgagcaaaaa ctagtagata
     1921 gtaatttaat tttagataat agtaaacagc aaaggaaatt agttatataa atactaattt
     1981 cctttgtttt atgttttaac atgattactt ttaaataaaa ttgtatttta tgaaaaataa
     2041 ttacactaaa tggcatagta ttatggggat ttttagtttt tattcaagta aatgaaatca
     2101 tattaacaac ttctgatata tttacaaatt gataaaaaaa tgattttatt agtatgatat
     2161 aatttagcta gtatgtatga taatggagga tatagggatg aataatatat tacttttgga
     2221 agatgacaaa tctttaaata gaggaattag ttttaaatta aaaaaagaag gatacaatgt
     2281 ttttagtgca tttggtatag aagaagcaaa aaatattttc gcaaaagaag agatatgctt
     2341 aattatttcc gatataggtc tacctgatgg tagtggtttt gacttttgtg aggagattag
     2401 aaagaaaagt gatgtttaca taataatgtt gacagctctt gatgaagagg tggatattgt
     2461 cacaggatat gatttaggtg cagatgatta tatcacaaag ccttttagct tgatggtatt
     2521 aatatcaaaa gtgaatgcgc ttatgaaaag agtaaataca gtaaaaaatt atactctatt
     2581 agtttgtgat gatttattct tttattatat agaaaataaa ttaattataa gaacagatga
     2641 taaggaagaa gagataattt taagtaagac agagacaaaa ctattaaaat atttgatgga
     2701 aaattctatg cagacattga ccaaagagca actgttagaa tctttatggg atagtagtgg
     2761 taattttgta gatgataata cgattgcagt aaatataaga agacttagac aaaaggttga
     2821 aaaaaatcca tctgaaccta aatatataaa aacagtaaga ggggttggat atatgtgggg
     2881 agaaaggagt ataaaaaaat gctaaaaaat atgaacctca aagatgtttt aagtgtgaaa
     2941 gatgattata taatgagaag agttattgta tattttgttt tatctgtact aataataacc
     3001 cttgtattta tactttttga atatagcaga ttacataaat tgtatgaaca tcaaataaaa
     3061 aatactcaaa atattgctgg aatattaatt gaaaaatatc ctaatgatga aatagatata
     3121 gtaaattcta tttataattc tgattatagt aaggtgtcta ctggagaaaa ggcatttaag
     3181 aaatttggct atggattgga aaataaaatg tcaaatgata aaaattttag cttatactta
     3241 aaatcttttt ttaaggaatc attttttatt tttttaatga tgtttatagt agttataggt
     3301 ataatatatt attttataag atacataaat aaaagattga gtaaaattta ttttatagta
     3361 gataagatgt cacaagaaaa ctacttgaag gacactactg agtcagaaga aactggttat
     3421 ctagtagaat ataatgaata ctttaaagaa ggcatattta gtaagataaa taactgtcta
     3481 tatgagctta atagaagttt aaagataaag tttattaaat tagaaaaaga aaaagaaagt
     3541 gtaaaatcat tagtaacaga tatttcccat caattaaaaa ctcctttagc atccttaaaa
     3601 ttatacaata cattacttat agaagaagaa ttagaagaag atgaagccaa cgagttctta
     3661 ttaaccaata aggcttctat aaataaatta gaaaatttaa taaattcttt agtgaacata
     3721 tcaaggttag aaattagcat gataaacata aaaaaagaag ataatgatat aaaaaatact
     3781 attctaaatg cagtaaaaag tgttacccct aaggctaaga gcaaaagtat aacattaaat
     3841 ttaaatgaat ttaacagtag aattatacca catgataaaa agtggactga ggagagtatt
     3901 tttaatgtat tagacaatgc tgtaaagtat acacaggtaa atggagaaat aaatatatct
     3961 gtagaagaaa ctgctaattt ctttaaaatt ataatagagg ataatggcat agggattgaa
     4021 aaaaatgagt ttaataatat atttaaacgt ttttatagag gttctgctga tgaaattgaa
     4081 tctgtagaag gtagtggggt aggattgtat ttaagtagaa agattttaga agaacaaggt
     4141 ggaaatatta tagtttcttc aaaagtagga atagggagca agttttcttt gtttcttaca
     4201 actatgtaat attaaatgaa aggaaaatgt aagcctctac atttattata taagtataaa
     4261 cagctccaga aataaaaaat taataaaata tggggtaata taacttagaa tgagaggata
     4321 attttatgaa aatactatat actgaaaatt tatcaaagca ttatggaaaa ggagaaagct
     4381 tagtaagagc tttggacaat gttgacttag aaataaatga aggtgaattt gttgcaataa
     4441 taggaaaatc aggttctgga aaaagtacac ttttgcatat gattggaggt cttgatatac
     4501 ctacatctgg gaaggtgtat atagataata aaaatatatt tacattaaaa gaagaagaac
     4561 ttgctgtatt tagaagaaga aaaatagggt ttatatttca aagctataat ttaattccat
     4621 ctcttaatgt atgggaaaat gtagtgttgc caatcggttt agatggtaga gaagtagatg
     4681 aatcttttat aaaagaactt ttaaaatcat taggacttga aaataaacac gacgttttac
     4741 caaatacatt atcaggtggt caacaacaaa gggtggcaat agcaagagca ttagctacaa
     4801 gaccagctat aatacttgct gatgaaccaa ctggaaacct agattctaaa acgtctgatg
     4861 aggttatgag tatattaaaa tctatgtcta agaagtacag tcaaactcta gttatgataa
     4921 ctcatgatga cagtatagcc caaatggctg atagagttat attcatagaa gatggcaggg
     4981 tgtcaaaggt tggtgataaa aatgattaat actactaaaa tagcaaaaag taatttaaag
     5041 caaaataaat caaaaagtat tctaataata attactataa tactttcaac cttaatgctt
     5101 tcttctattg gaatttatat tgttgatgca ggagcatatc aaaaagaaaa tactataaag
     5161 tattcaggca attatcaagg tatattagca aatgtagatg aaaaacaggc tgatatattg
     5221 agtaatcatg cagatgttga attaactggt gaaatgaatg gagtaggtgt tgaaaaactg
     5281 gaagatgatt caaatatttc tctagcatac atgaatgaag atgctttgaa attaaatagt
     5341 tttgaattta tcaaaggaaa attgccatca aaagagaatg aaattgtgtt agatagtgga
     5401 gctttaaagg cacttggtta taaaaataaa gatttaggtg aaaagataaa aatatcttat
     5461 gatgactata aaagtgataa aaaaattgaa aaagagttca ttataagtgg aatcttaaaa
     5521 actagcgaaa tatctgaagc aggtaaatat tattatgcta ttatatcaga atcatatatg
     5581 agaaatacta gagatatgtc gcaagaagat tttaatattt atgtgaaatt taatgataaa
     5641 agtaatttat caatagagca agcaaaagaa aaattagata aaattgctaa tgatatagga
     5701 ctggatacta ttaatactgc tgtaaatgaa aattatataa atgctttaaa accagatatg
     5761 gaaacgatta tgggtggagt ttttgtaggg ttagtaattg tactatctag tattttagta
     5821 atatacaata tattttatat ttcaatagtt acaaaggttc aagaatttgg aaaacttaga
     5881 gcaattggtg caactaagaa acaaataaaa aatatagtgt ttaaagaagg gttcatatta
     5941 gccggaatag caataccaat aggaataatt ttaggctatg tacttgcaaa tattataata
     6001 aaatcattta tggatataga tgtaaaatca tcacgattac cagttatatt atcggttgtg
     6061 gtaataagct ttatatctgt tgttttatca cttttaaaac caatgaaagt agcatcaaaa
     6121 gtatccattg ttgacgcagt aagatataca ggaaataaaa taagtaataa gagtaagaga
     6181 aaaggatata aaaatataaa tctaaagaga ttatcacatg ccaatttaga aagaaataaa
     6241 aaacgtactt acatgacatt ggcatcactt atacttagtg gaactatatt tataacagta
     6301 tcaactgctt tagaaagttt tgatgctgag aaaatggcaa gagaacactt tccttatgat
     6361 atagaagtta gactatctgg ttatgaaatg aacagtgata aaaatcctaa aaataatttg
     6421 aatattttac aaatgaataa tcctctaagt aaagattttt ttaatcaaat aaagaatatg
     6481 gaaggaatta aaagaataga atctgcaaga agtgtaaaaa ttggtatgga ggactatgat
     6541 gtcgaattta agtatgacct tttacaaagt ataaatgaaa atgatgtaaa atcattaaac
     6601 aagaatataa tagatggaaa aataaatctt gaaagacttc aaactggaga tgaaatagta
     6661 attactcatg ttgatacagc aaaagaaatg ggagtaaaag caggagataa aataagatta
     6721 actttatatg atggagataa aaagataaaa aaagaattta aagttcaagc aatagctatg
     6781 ggggttccaa gttttggtat aggaaaggat tttattgata gaactttgaa atacgattca
     6841 actagtagct tgggaatata tacagaagaa ggaaagtatc aagaaatcaa agatagtatc
     6901 aagaagattg ctaaaaataa tggattttta gagactgatt ttatagatag tagaattgag
     6961 tcaaataagg ctactatttc atttataaag ataatgggat acacgctaac aggcataata
     7021 ggtgtgattg gatttatgaa tcttgtcaac acaatgataa ctagcatagt tactagaaaa
     7081 aaagaacttg gtatgcttca agctattggt cttactaata aacaattagt gaaaatgctt
     7141 aattcagagg caatgtatta cacttctgga atgatgatag gaagtattct atttggaggt
     7201 atattagggt atattgctgt tatatttcta aaaaagactg gattatctta tgcaacatat
     7261 tctttgccaa tagtaccaat cttgttgatg attgtgtgta ttcttatagc tcaatttata
     7321 acaacttatt tgataggacg aagttttaat aaagaatctc ttattgatag agtaagatac
     7381 agtgaataaa atgcaaatat ttatggttag tttaactagc aaaaaacata taaaaaaata
     7441 tagttaaaga gattcataat taaataggtt aaagcatagt acagagcaaa caatttaaac
     7501 agatgtttgc tctgtatttt attatgtaat caataatata tctaatatac tgatattatt
     7561 catgttagaa aatgtaccgg tacaattcta aaaaagtata aaattgtacc atataaaatg
     7621 aaaattttgt ataataatcg tatagaaaat aaataggagc tgatatatat gaataagaag
     7681 attaattatg caccaggacc aactgaaact agggaaaatg taagattagc aagagcagaa
     7741 aaaacaacaa atccagatat agatattgat tttgttgaat tttataaaaa gacatgcgag
     7801 aagtttggta atataattgg aacaaaaaac gatgtttaca tattgagtgg tgaaggaata
     7861 ttaggtcttg aagcagcatg tgcatcactt acagaaaaag gagatagagt acttgtaatt
     7921 gataatggta tatatggaga aggttttaaa gattttgtaa caatgtatgg tggggagtat
     7981 gtgctttttt caagtgaata tactaaaagt atagatattg atgaattgaa agcattttta
     8041 gatgaggata gtaattttaa atatgcaaca gtagtgcatt gtgatactcc aactggcgta
     8101 ttaaatgatg taagcaaaat atgtccatta cttaaagaat atggaatatt aactgtagtt
     8161 gattctgtag caggtatggt aggagaaaga ttaagtgtag atgaatctaa aatagatata
     8221 atattaggtg gttcacaaaa agctatttct gcaccagcag gacttactat agtcggaata
     8281 agtcaagatg caaaaaattg tataaaaaat agaaaaactg atgtaatagg tttttattgt
     8341 aatttaaata tatgggaagg ctattatgaa aaaaaatact tcccttatac aatgccaata
     8401 agtgacataa tggggcttga taaagcactt gataatatat tagaagaagg aatagaaaat
     8461 gtattaaata gacatgaaaa gatagcttat agtgttagaa aatctattga ggaatatggt
     8521 ctagaactat ttttagaaga aggatattct aatacagtca cagctataaa agttcctgaa
     8581 agtataggag ctttagaact tactgattat atgcttaaaa attataatac acttgttgca
     8641 acatcactaa atcaatatat ggatactatt ttaagaatag gtcatatggg agagaatgca
     8701 aatctaaata aaatagaaca tgtactaaat gttttggata aaagtttaag tgcactagga
     8761 ttcaaaggaa atggaaattt gttaaattta tttaataagt attattttta atagataggg
     8821 ggatatagta tggaagttac agctaagcaa aaatttacaa cgaaagacat ggtggaaacc
     8881 tcactattaa tagcattagt atttattgca actaaattta taaacataag attaccaata
     8941 tctataaatg gtgggcttgt gcatttggga actgcaatgt tgttcatatc agcaatagta
     9001 tttggaggta aaaaaggtgc tttatcagga gctattggta tgtcactttt tgatttgata
     9061 tctgggtgga ctttatgggc accgtttacc tttatagtta gaggagttat gggttattta
     9121 cttggaaaga ttgcatgggc aaatggtaat aatgggaata attttttaat caatgtaata
     9181 ggaatatgtg tctcatcaat ttggatgcta tttggatatt atgtaacaga agttatactt
     9241 tatggtaatt ttatagtacc tcttacttca ataccaggta atttaatgca agtattgata
     9301 ggtcttataa tagcgcttcc tatttctaag gtattgaaaa aatgtataaa ataaatataa
     9361 agtgaactaa aatttatgat agcataaatt atactgtata atattaggat ggagtacata
     9421 attaaaaaat tttagtatag aggttagttc acaatgttat cagttacaag atattcaatc
     9481 aaaaatgttc gtttaaagcc gtgggtaaag tttatttggt attttgagac aaaatcaaat
     9541 atactgttaa ataataagtt acttccgaca gacagtatag atattattct taatctttct
     9601 gattttatgg aatataaaat tgaaaatcaa gactatactg ctagtaatat gcattttaat
     9661 ggaataaggg ataaacatgg atttattatt caacatggaa atatacgtgt tataggggtt
     9721 tctttttatc catttggttt atatccattt ttaaagatac caatttcaga gtttaacaga
     9781 caaattgttg acctagaagc agtatcaaaa ctatttgcaa aaaaattaga agagtcatta
     9841 agtccaacac aatcagtaga aaaagttgtt ttatgtttag aagaagtatt attatccata
     9901 ttagaagaag atttaatttc aaataaatat gtaaagcttc taaattcatt tatatatgtt
     9961 aatagatata gcaatataaa ggcattttgt gatgatacaa atataaatat taaaacattg
    10021 gaaagaatct gtttaaaata tacagggtat acacctaaga ttttaaagag aatttacaga
    10081 tttaaaatgg caagcaatca acttatttat aattataaag atgatgaact ttttgatttt
    10141 atttatgaaa atgagtacta tgatcaagca cactttataa aagaatttaa aaaattttca
    10201 gggacgtcac caattaaatt tattggtgag aataagacga ttaaagaaaa tataaagtac
    10261 agctatttat aattatactg tcgatttttt acaatacctc caatttattt tttgatatga
    10321 tataggaaaa taaattggag gttttacata tgaaaattat tacagaaata gttgagttta
    10381 atgtagaaga aaatttatca aaagaagagt ttatagaaat tgtaaatgac ttagaattaa
    10441 aatttcattc tattcaatct ggttttattg atactgagct tttatatgat gaaaagtctc
    10501 aaaagtggat tatgatacaa cattgggaat ctatagaaga cctcaagagt gcttctagta
    10561 aaatgtttaa agattcttca actgagaaat ttagaaaagc tttaaagaat caaactgtta
    10621 agatgaatat aataccacaa attaaaagtt ggaaattata gaactgaatg taaaagatag
    10681 ttaaatagta tcaaactaaa ttagccaaat aacattttgt atttaaattg ttatttggct
    10741 tttagtgttt attttaatat gagtgttata tactagaaaa accaacaaac tgttagagtg
    10801 tggattatca taatgttata ttcgggagct aaaaggctta atactgattt gccaattggt
    10861 caatccatgc atgtccatct ggatatgctg ttattaaagc atttacaagc caatcccttt
    10921 gctcttgtga taatagtggg actgttacat cgaagtcttt ttgataacca agcctatcta
    10981 tgtatattga tttgcaaaac aaaaccactt cgggagccat atatgggata ttatctgatg
    11041 ttatcagtct ggctttatcc atatctctta taattctttg attttgggaa aaaagaaatt
    11101 ttccatcttt ctgtggatta tatatcaatt caataaaatc gaactgtaac tgttcgtcac
    11161 actgtatatt cattttatag ttatcatcct cgactttttt taattcaata aatgagcaat
    11221 caggcttaat cgcaaaaaca caaatttgct ctatatgttg cttgttaagg tcgataatag
    11281 gtcgtaataa ataattaccc aagggctcat atatatccca tccttgatta agcatatact
    11341 gaatgctctt ttctttatct gtgtcagaaa tccatatgtc aatatcactg tgtgggcgaa
    11401 tatgcatatt cgcaaataat tctagggcat atccaccaca tattgaatat gaaaagccac
    11461 aatcattaaa aaagtttaca caatctttaa ttagtttgtt catagcaacc tccttttgca
    11521 tgtactgaaa taaattaaat ttttaaaaat accaactaaa tgtttgtttt gtgtaaatat
    11581 aaaactgccg atattaaggc agttttatat ttactattta gaaaatttat taacaaattt
    11641 ttgatattca cacattatct tatcattcat aaaagaaata cccataaaag cagcttgctc
    11701 accaaacaaa gtaaaacgtt ctatcatctg ttgtggatta cctggataaa ctgttggaat
    11761 acaccatgtg tttagaagaa aaccaaacat ttgagcagca atctctggat ttttaacttg
    11821 catagaatta tcttgatttc cttcttcaat taatgctttt atccaaggca caatatcatt
    11881 tataatctca tcaaactgcg tagctaaata cttaggatta cgcaatagtg atgatgacat
    11941 taaaaaaatg tcttgtttat tgtgttgttc ataagcatga agcaacctat attgtaattt
    12001 ttctaatgcg gtctttcctt catcattttt tacatcatat ccataaaatc catcattata
    12061 caaacgactt gtgattgcct taattatttc gtcttttgat ttaaagtgat gataaatagc
    12121 acctcttgaa aggccatcta aattatctac aatatcttga atcgttgtct gctcataccc
    12181 tttttctaag aacaacttat gtgaaatatc aagtattttt tgtatagtat tatcaggttc
    12241 tttttttctt cccaaaaaac tcactcctca aaacaatcat ttggtatgtt tatcttagca
    12301 aatacataat gatttgtcaa tgtgtctttt tagcatgttg aacaggtata ttgccatgat
    12361 ttcattattt tatttttttg ccttatactg gactatattt gcagtttcct aatatatttg
    12421 gctcatattt aaataccatt atctaaaagc actattgctg tacgagaggc aataggcatt
    12481 ttagaagaag gaggagttga gctaaagtat acatcataat ctcttaagtt ttcttttttt
    12541 gtgtttcctc caaactctac catatcagta tttaatatcg ttttgtagtt tgtattttga
    12601 aactttgaaa tatcaactga ttgtattaga ttagggtgga aattaaatat aaataaatat
    12661 ttgcccttac tgtacaccaa aatcttttta tcattatcca tatgtataag ttcatgacta
    12721 ttttggataa aaatatcact gtattttgta agttcgagca tagatttatc aaaacttaat
    12781 aattgtttat actttaagtt gttatcttct gaaaggctcc attgtcttct ggcatactta
    12841 tagctccaat tgttgccttc tcgtggaaaa tcaatccact caggatgtcc aaattcattt
    12901 cccataaaat ttagatatcc ttcacctgct agagaaaaag ttattaattt aattaattta
    12961 ataagagata ttgctctatt gataacatgg ttatttgagt ttatttccat attccagtac
    13021 atttccttgt cagctagcca aaaaataatt gttttatcgc caaccaatgc ttggtcatgg
    13081 gattcacaat atcctatatt tttttctcca ggtcgtctag tagtaagttc ataccacatt
    13141 ttgcccaaat cccagtcttc atcagataag ttagagatag tttttatcca aaaatcaggt
    13201 actcccattg cgagtctata gtcaaatcca attccgccgt cttttatagg gatacacata
    13261 ccaggcattc cactcatatc ttctgcaata cttatcgaat taggtttaat ttcttttatt
    13321 aattcatttg caaattggag atatgttata gcttcaatat ctgtattcat actaaaatat
    13381 ttttcataac tatcaaatga aactccaaga ccatggttat gatataacat tgaagttaca
    13441 ccatcaaacc taaagccatc aaaatggtac tcatttaacc aaaattttat atttgaaagc
    13501 aaaaaatgta ttacttcagg tttaccatag ttaaaaagtt ttgtacccca agcaggatga
    13561 tttccctttg aacctgagtg gaaaaattga tgttcacttc catcaaactc atttattcct
    13621 tctaaagtat tctttacagc atgagaatga actaaatcca ataaaacagc aatccccatt
    13681 gaatgagcag tgtttatgag gttttttaag tcttcaggag taccaaacct agatgagatt
    13741 gcataaaagt ttgaaacttg gtatccaaaa gatgcataat atggatgttc cataatagcc
    13801 ataagctgaa tggtattgta tccagctttt tttatcttag gaagtatttt ttcagtaaat
    13861 tcattataag ttccaataga ttcgctttca gtagacatac ctatatgaca ttcatagatt
    13921 agtggagaag ttatgttttt taagtcaaaa ttattgtcag tccagataaa tggagttttt
    13981 ggttgccata tttgtccatt aaatccacca aaatcttttt gaattactct ttttatatat
    14041 aatggtatac ggtcaaatgt ttttccattt gcagtaactt gtacctttac ctctgatttg
    14101 tgaggtaagc tattttgacc aggaataaat atttcccaat taccagaccc aatttttctt
    14161 agaggatgag attttctatt ccaattatta aagtcaccaa ttagtgacaa actatctgca
    14221 tttggagccc attccctata gaagaatcca tcatttgtct gatgaaatcc atagtaaaga
    14281 tgagcatttg caaaagaact aaaatcttta tagtttttca atgtttttga tttcatatcc
    14341 tcataatgtt tcattcgctt ttttatatca gattcaaatg gttgtaggta tacatcaata
    14401 tctaaaattt taaatctttt ttgacttttc attgtatttc tcctccataa taaatattac
    14461 atctaaaaaa taatatttat ctcatactat cttctctgta taattgatat atattatata
    14521 atcctaataa aaagcttaag ccaccaatga ttaatttcat aatttacctc cctagtattt
    14581 atacttatat atcgtattca tcaaaattta aaagtttgtt taaaactctt ttaataatat
    14641 cataatcaaa gcctttatag gaaagatgtt gagaaatttt ttgatatatt ttctttttat
    14701 cttctttctt tactctttca tacctttttt ttgctagata catagcattt tcaaattcag
    14761 tgtccttgtc taattcagat actgcttcat tgattgtact tctattgata cctttattat
    14821 ataaattctg cttaattctg tttttgccac atttatttag gttaacattt gtgttaacaa
    14881 ttttttgagc taatacactg tcatttacga gattataact tttaagaaaa tctatgaccc
    14941 tctcaattat attttcatca aattctgatg atagtttttc ttttaatttt ttttcagatt
    15001 ggtcagcatg agataaaata tttaatgctt tatttttagc ttttatatac atttcatcat
    15061 ctaaaatagg ttttaattct tcttcattta tttcattccc tttttttaaa ttaaatgtat
    15121 aaacaagttc tttaaatata gcaataaaaa atttatcatc tacataaata ttaactctat
    15181 catcatttct tttttgttgt tctatttttg taattatacc cataagtcct ccttttttat
    15241 atgtaattat attaacaaaa gcaactaata aaatttaaca aaaaacttat atttgtatgt
    15301 tatattttat caaaattaag acaaaatagt aaaaagttat ttttattaaa aagtaaagca
    15361 gaatcatgtt aaataattat tttttaaagt gttttttagc atataaatat aattatatac
    15421 ccaaaaaaac aaattataag ctttaagaaa taaaaaataa tggtacaata gtattataac
    15481 attaaaagga gacaaaatat gagaagtgaa aatctagtta agatggcaga gttgaaaaat
    15541 actgaaaaac tagagaaatt taatcgccat aaaggcaaaa tcaaaatagg tatattggga
    15601 ggtacttttg accctataca ctatgctcat ttagcaacag cagaatttat tagagacaag
    15661 tatgacattg acaaaataat ctttatacca tcaggaaatc caccacataa attatgtatc
    15721 actacagata agtatgatag atataatatg acacttctag caactgaaag caacgaagat
    15781 tttttagttt caaaagtaga aatcgaaaga aagaaaagga cttatacaat agatacatta
    15841 aaatacttaa agaaaaaata caaaaatgca gacatttatt ttataacagg tgcagatgca
    15901 atttgtagtg ttgaagagtg gaaagatgta aagaaaaact ttgagttagc tacttttata
    15961 gccgcaacta gaccaggaat tagtttgtta aggtcacaag agactataga aaaactaact
    16021 aagaagtata atgctgacat tataacagtt tatgttccat cactggatat atcgtccaca
    16081 tatataaggg aacagctaaa tgaaggcaag tcaatacgtt acttagtacc cgaaaatgta
    16141 gaaaattatt tatatgaaaa taaactatat caatatggag atgattaaat gaacttagaa
    16201 aatattaacc aaagactaaa tcaaatgcta ccagtgggga ggcttagcca ttcaaaaaat
    16261 gttgcaaaat gtgctgaaaa attatgtgag atttatggat gtgacaaaga aaaagcttac
    16321 ttagcaggaa tgattcatga ttgtgcaaag tatttaagtg acaaggaaat agaggattat
    16381 gtaaataagt atgaaatata tttagaccca atagaggatg gaaatcgttc cttgtctcat
    16441 agtgtaattg gggcgtacat atgcgagtat gaatttgaag tagaagatga agatattata
    16501 aatgcaatca aatatcatac aacagggaga gaggacatgt ctcttttaga aaaaataatt
    16561 tacatagctg atttaattga agaaggaaga aaattcccag ttgtagatac attaagagaa
    16621 ttagcatatg gagggaagct tgatgaagca cttttaactt cttttaataa tactttaatg
    16681 tttgtcatca ataaaaaaga agagatacat ccaagaacag ttatggcaag aaactatcta
    16741 attaaagaaa agttattata aaaaagctat ataaaggcaa acatattaat aaatttaaat
    16801 ttgactatgt tacaaaatcg tattaatatg ttacaataac taagtgtatt ttaattagga
    16861 aagtgaggaa tttttatatg acagtggaac aaatgacaaa aatagcatat gatgccatag
    16921 aagataaact gggtcaagat acagttataa taaatatagg aaaagtttct agtttgtgtg
    16981 attattttat tattactaca gcatcttctc agagacaggt aaaagctatt gctgacaatg
    17041 tggaagatga attagcaaaa ctaggattag agccaagagg taaagaagga caaggaactc
    17101 aaacttgggt actccttgat tatggagaca taatggttca tgtatttaat gaggaaaata
    17161 gagggtttta taatttagaa aaattatgga aagatgcacc ttatatagat attgacacat
    17221 tagcataaac atgataatat atgttattat aattagaaaa tttcaaaaat tatattaaat
    17281 tgaatagact agagcagtaa aagattatat tttttcagag agttagtgga aggtgagaac
    17341 taatattata taactttgaa cttactagat taaaatctat tctccatggc tggattaaaa
    17401 gctatctaag agagtctata tatgtagtag gctaattagg gtggtaccgc gaagatttat
    17461 cctcgtccct aaacgtaagt ttagtgacga ggatttttta ttttcaaaga attatttgga
    17521 aattataagt aaataatact aaataaggta gttatacaca attaaaaata ggaggcaaaa
    17581 tatgagcgta tataatttta aagaagtaga atcgaaatgg caaaaaattt ggaaagataa
    17641 taatcagtat aaaatggata ctgcacaaac tgaaaaacct aattattaca cattagaaat
    17701 gttcccttat ccatctggaa aaatacacat gggacatgtt agaaattatt ctataggtga
    17761 tgttgtcgca agattcaaaa aaatggaagg ctataatgta cttcatccaa tgggatggga
    17821 ttcttttgga ttaccagctg aaaatgcagc tataaaacat ggaatacatc cacataaatg
    17881 gacaatggaa aatatagaag aaatgaaaga gcaattaaat ttattaggta ttagttatga
    17941 ttgggataaa gaagttgcaa cttcaacgcc agaatactat agatttacac aagaaatatt
    18001 tttgaagttt ttagagcatg ggcttgcata taagaaaaaa tcatatgtta actggtgtcc
    18061 ttcttgtgag actgttcttg caaatgagca agttgttcaa ggagcatgtg agagatgcaa
    18121 agcaacagta ttaaagaaag atttagaaca atggtatttt aagactactg agtttgcaga
    18181 agaattactt aatgacttag atacattaga tggatggcca gaaaaggtta aaacaatgca
    18241 aaaaaattgg ataggtaaaa gtacaggagc agatttagtt tttgatatag atggaactga
    18301 taaatctatg acagtattta caactagacc agacacaact tatggagtta cttatatggt
    18361 acttgctcca gagcatgaat tagttaaaga attggttgca gggactgaat atgaagcaga
    18421 tgttgaagct tttgttcaaa aaatgcatac tatgacagaa atagaaagaa cagcagctga
    18481 tgtagaaaaa gaaggaatgt ttataggtag atatgttata aatcctttaa atggcaagaa
    18541 agttcctcta tggatagcta actatgtatt agttgagtat ggaacaggtg ctataatggc
    18601 agttccagca catgatgaaa gagatagaga ttttgctgaa aaatataatt tagacataat
    18661 tgatgtaata acagaagata acaaaatgat aaattcagaa gaatttaatg gattagatgc
    18721 tttagaagga tttgaaggaa taatcgataa gttagaaaaa gaaggtagag gaaagagaac
    18781 aattaattat agacttagag actggttagt ttcaagacaa agatactggg gttgtcctat
    18841 acctgtggtt tattgtgatg aatgtggaat agttccagtt aaaaaggaag atttaccagt
    18901 tcttttacca acagatgttg aatttactgg aaaaggggaa tctccactta cgacttcaaa
    18961 gcaatttatg tcaacaactt gtcctcactg tggaaaacca gctagaaggg aagttgatac
    19021 tatggataca ttcgttgatt cttcttggta tttcttaaga tatgtggata gcaataatga
    19081 aaatgaacca tttagtaagg aattagttaa tagatggcat ccagtagacc aatatatagg
    19141 tggagtagaa catgctataa tgcatttgct ttatgcaaga tggtttgtaa aggcatttaa
    19201 gagtatggga atggtagact tcaatgagcc atttaaaaac ttacttactc aaggaatggt
    19261 acttatggat ggttctaaaa tgagtaagtc taaagggaat acagtatctc ctatggatat
    19321 aatagatgag tatggagcag atactgcaag acttttcgta ttatttgcag caccaccaga
    19381 aagagattta gactggtcag agcaaggtgt tgatggatgc tttagattct taaatagggt
    19441 ttatagattg gttgacgagt tagcagatgt ggttaaaaag gatgtagagt ttggagaatt
    19501 aaattcccaa gataaggata tgagatatac tatacactct acacttaaaa aagttactgc
    19561 tgatttaagt gaaaaattcg gatttaatac tgctatatca gctcttatgg aattaataaa
    19621 tgatatgtat aaatataaag agttagataa tataaacgaa gcagttataa aagaaggtgt
    19681 acaaactata gtaactataa ttgcaccatt tgcccctcat ttaggtgaag aactatggac
    19741 tatgataggt aaagaaggaa gtgtatttga tatagactgg cctaaatatg acgaaaaagc
    19801 tttagtaaaa gatgaaatag aagttgttgt acaagttaat ggaaaagtta gaggaaaatt
    19861 aactgttaac tcaaatatat ctaaagatga gatggaaaag gtagctttag aagatgaaaa
    19921 aataaaagga ttagttgaag gtaaaactat agttaaagtt gtagctgtac ctaaaaaatt
    19981 agtaaatata gttgttaaat aactcaaaaa tataaacaaa aatataaatt tataaaataa
//
LOCUS       DACXMZ010000053        19951 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.053, whole genome shotgun sequence.
ACCESSION   DACXMZ010000053 DACXMZ010000000
VERSION     DACXMZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.053"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..756
                     /locus_tag="JD016_07465"
     CDS             <1..756
                     /locus_tag="JD016_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAU4999478.1"
                     /translation="AGSAVANKRAERKNTASKENRGNTTSSMGQRAGSKVGAVLDTKN
                     KVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKRGMVREQQSRQTGRLEKQE
                     QHKKNIADKRMELQKAQEARQAQRKADGSATTGATRPHERPATASKPSAEKMQEVKRP
                     ATATTSKASEPVKTNVIKERPLSSGASDRKATQSAQPVHRQNVEKVVSQETRQNYPKD
                     RRTKVQQTQSVQKNQQTTEKTRNLVTKKGQKKK"
     gene            753..1760
                     /locus_tag="JD016_07470"
     CDS             753..1760
                     /locus_tag="JD016_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAU4999479.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVAHFDNATAQAIM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYADLSSSYWQQHLIGAGRVK
                     Q"
     gene            1777..2688
                     /locus_tag="JD016_07475"
     CDS             1777..2688
                     /locus_tag="JD016_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU4999480.1"
                     /translation="MFKKNKKQTETLKEPKEKKVRTVKVGTHKKTVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWNNSKEAIEARTQA
                     INGYLTKELQDLNVDTIRTDIPTSSTVTDVIVWSIEQSGTDTFSATYEVDQQIKEGEQ
                     TSNVKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDYEPKTPEADASVDADTVNDATS
                     FLETFFKLYPTATEKELAYYVTGNVLEPIGRDYLYSELVNPIFTKDGDNVKVKVAVKF
                     LDNQTKATQVSQYELVLHKDSNWKIIG"
     gene            2811..3185
                     /locus_tag="JD016_07480"
     CDS             2811..3185
                     /locus_tag="JD016_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU4999481.1"
                     /translation="MDIFINNHASIPIYEQISSQIKAMIINGDLKAGEALPSIRGLAN
                     SLHISVLTVQKSYDVLQKDGFIETTAGKGCFVSVQNQDFYLEEQQKKIENYFIEAIEI
                     AKVSGIKLDKLQSLLALLYEEE"
     gene            3190..4044
                     /locus_tag="JD016_07485"
     CDS             3190..4044
                     /locus_tag="JD016_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4999482.1"
                     /translation="MEKYALELNGVTKQYSDFKLDNISFTVPQGSIVGLIGENGAGKS
                     TTIKAILGLIVTEQGKISVFNGKDVNAETKEHIGVVFDGSNYPDNLTPKKLGKVFQNI
                     YAEWNNEKYFELLNKFELPINKKLKEFSKGMNMKYSLVVTLSHNSKLLILDEATSGLD
                     PVIRDDILEMFLDFIQDEEHSILVSSHITSDLEKIADYIVFIHEGKVLFSRPKDELTE
                     QYGIIKCNAKQFDELNKNDIIRYKKQDYEWQVLVANRQKAKKEYPHTLVVPASIDDIM
                     LLYVKGEC"
     gene            4038..4652
                     /locus_tag="JD016_07490"
     CDS             4038..4652
                     /locus_tag="JD016_07490"
                     /inference="COORDINATES: protein motif:HMM:PF13346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAU4999483.1"
                     /translation="MLIMKGLLLKDYFAIKSSMIILLIGFVVFGFGVSFYAPPYVLML
                     IASIILGQFVTTTIYSDRKCGWLKSSITFPTSKKSHVSSKYIMYALLCLLGVVVGLVL
                     SIIITSIRNELDTHLLLLFTCMTTTMIFMSGAVLIPCYYFIKEELSGAIGILAYGISG
                     VFFFGMIKLIENHVLALTISIFTAIILFVISWCFLCQQIINRDI"
     gene            4663..4893
                     /locus_tag="JD016_07495"
     CDS             4663..4893
                     /locus_tag="JD016_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999484.1"
                     /translation="MLRIKKRDIKKALKAGAKAGGMSLADVRENIEATIDEAMNSTDP
                     EVQANFKKYFGNKRPTPEEYIYIITKKTKVKV"
     gene            complement(4951..5274)
                     /locus_tag="JD016_07500"
     CDS             complement(4951..5274)
                     /locus_tag="JD016_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999485.1"
                     /translation="MENNCCGFSCPVEATIQLIGGKYKAVILWHLMNKTLRYNEIHKL
                     IPKATDKMLAQQLRELEKDGLINRKVYPVVPPKTEYSLTEFGKTLTPILDELCNWGEN
                     YLLQK"
     gene            5399..5917
                     /locus_tag="JD016_07505"
     CDS             5399..5917
                     /locus_tag="JD016_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HAU4999486.1"
                     /translation="MNVLDIAKKRYSVRTYEDKPVEKEKLEKILASAHVAPTAANLQP
                     VKLLVVQDKERLKSIAKAGNIYNAPLAIIVCADHSKAWTRPFDNKQTTDIDATILTDH
                     MMLQATELGLGTVWVCFFKPNVIKREFQLPDNLEPVNILVIGYGKETPADPERHFATR
                     IPMEELVSYDYL"
     gene            5968..6042
                     /locus_tag="JD016_07510"
                     /pseudo
     CDS             5968..6042
                     /locus_tag="JD016_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6117..6887
                     /locus_tag="JD016_07515"
     CDS             6117..6887
                     /locus_tag="JD016_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4999487.1"
                     /translation="MEHILNLEQVKKYYGGNSGNITKAVDGISMYVDKGEFVAIMGAS
                     GSGKTTLLNCISTIDTVTSGHITVNNQDITKIKDKDFADFRRENLGFIFQDFNLLDTL
                     TIEENISLSLVINKQNPADIDKRVHAIADKLGIRDILSKFPYEVSGGQKQRCACARAL
                     INEPKLILADEPTGALDSKSSRLLLETMSDINKKMQATILMVTHDPFSASFCKRILFL
                     KDGKIFNEIFRGEKSRKDFFNEILDILTLLGGEVGNVR"
     gene            6877..8856
                     /locus_tag="JD016_07520"
     CDS             6877..8856
                     /locus_tag="JD016_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4999488.1"
                     /translation="MLGKLAVRNTKRSIKDYLIYLITVTIAFSLIFAFNLVASSEEVI
                     ELSTGMDTFKYILAFVNVVIIFVICFLINYTTKFMFEKRSKELGTYMLLGIRKKEIAR
                     LLVIENVLLAICALVLAIPLGFIFSQFVSLVIVNLLGIPEVIFISINFVSIGLLAIYF
                     LAIYVLVLLNLLRRIRKMTVHDFLYFDKQNEKKMFRSNKKRNIIFIVSVILGITAILL
                     WASRWTMENSGKQETLTYLMISMMMLIISIYGVSATCADMLLSVLLKNKKLKYQKDNL
                     FVTRTFASKARTMSFTFGTLSMLILLSLLCLNFSSINKGVYKASIEMTAPFDVHVFEE
                     KQPFKDFKEYVNVVDEDYTINKAIEFDVYKEPKHQMQNYFDVQFYDYDPVMKLSDYNE
                     ILKLKNMDTIELSNDEYFLVTSKDLLYKVENNKDIEKIQLTSGKELKLKGIDTKTYWY
                     QINNTGRFAVIVPDEYVQGLEVSEQHLIIDTIEETDTKLREKIKQDLKHRLVIVNDDG
                     ETVVQYYRLSVRGSAIEEQNTMTAMIASICLYIAFILISAVGTVLAIQSLSDSTKYKY
                     RYQTLKRLGVNDKSLFKTIRKQLLILFGVPVIYSILASFFMLVSVNNVYKIYLESEYS
                     YLIYFVVGLAIFFFIYGIYWIATYIGFKRNINEES"
     gene            8915..9574
                     /locus_tag="JD016_07525"
     CDS             8915..9574
                     /locus_tag="JD016_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4999489.1"
                     /translation="MRIAIIEDDEITRLELSKLLNTQGYETVLLTNFENLTEELKQYC
                     VELVLLDINLPYENGYEVCRKIKQAMPVPIIFVTSRDTNADELKSIQVGGIDFITKPY
                     DTLILLEKIKRALQLSNPNNFRELVKKDCTLDLHLSILKYQEKSIELTRNEFRILYYF
                     FMNEDKVISKEELLEKLWNDKYYLDENVLLVNMTRLKKKMKEIGIVHLLENIRGKGWK
                     L"
     gene            9571..10572
                     /locus_tag="JD016_07530"
     CDS             9571..10572
                     /locus_tag="JD016_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU4999490.1"
                     /translation="MSFFTFLEEKITEILFQLFFLALVVFLLIFYGVDTLFVVLLVIL
                     FISIQGFFQWCLYRKKRNASQHIIDLVDGLEETYYIADVLPKPKEFQNEAYYYALKKA
                     CKSMNDEISKITEEKQDYQEYVESFAHEIKIPIGALSLTFDNTKNYTLKKETDKIFQL
                     VEQMLYYARSENTEKDYFVKQLNLDEVIHNIILKFRHSLMERKTVINIHDIENVVYTD
                     EKWLTFILSQIVQNTIKYFDKQENKLTIYSQDNETNILLVIEDNGCGIKASDLSRVFE
                     KGFTGSNRNKANATGMGLYLSKKLSDRLGLKLDITSKETEYTRLTITFPKGTVHNLH"
     gene            10620..10994
                     /locus_tag="JD016_07535"
     CDS             10620..10994
                     /locus_tag="JD016_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU4999491.1"
                     /translation="MELYLNDVYPYAQQISNYIEREIFQQKLKAGQKLLSITNLSKEL
                     NVSKHTIQLSYKILRKKELIVKKGNDFFVTKDNCCIITKMKEETDLQIIKCLHELAEM
                     GYSREQTKSLILRKLETLKFPQ"
     gene            11277..11705
                     /locus_tag="JD016_07540"
     CDS             11277..11705
                     /locus_tag="JD016_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAU4999492.1"
                     /translation="MRLMKPSDFQKTVQCRFESCLKKVVRSVVKDYYKELNRRKNKEI
                     SFSELPDVIVDKMTVWDDYETDYTIFSVCGIDIRVLDDELAEALKKLPERKRNTLLMY
                     YFLEMTESEIANLQKITQSGVFRNRHHALEIMKKILKEEH"
     gene            11709..11963
                     /locus_tag="JD016_07545"
     CDS             11709..11963
                     /locus_tag="JD016_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU4999493.1"
                     /translation="MKQLFKKPSYYLISSAMDGNEKAIEKLLAFYDPYISKCCLRSLY
                     DEYGNVYIVVDMELKGRIREALIKMILGFNIEDMDLEPEK"
     gene            complement(11858..12103)
                     /locus_tag="JD016_07550"
     CDS             complement(11858..12103)
                     /locus_tag="JD016_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999494.1"
                     /translation="MARYLTNVSVCFIQLSKSCSESVLLSCRSRGRAGKRGTLNHAPL
                     KTAYFSGSKSISSILKPKIILISASLIRPFNSMSTTI"
     gene            complement(11986..12224)
                     /locus_tag="JD016_07555"
                     /pseudo
     CDS             complement(11986..12224)
                     /locus_tag="JD016_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12334..12537
                     /locus_tag="JD016_07560"
     CDS             12334..12537
                     /locus_tag="JD016_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAU4999495.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENLDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            12616..13806
                     /locus_tag="JD016_07565"
     CDS             12616..13806
                     /locus_tag="JD016_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAU4999496.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYSWK
                     LVPTDKTPAGKRDDISLREKETQIKKDLNDGIDTAGGKMTVCQLYDKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSEKGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEQKVILTPEQEERLLAFMETDKIYSKYYDEIV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLKDSEIGYYIETPKTKNGKRELPLTE
                     RAYQAIQRILKNRGKAQPLIVGGYSNFLFLNREGLPKVAGNYEGMVRGLIKKYNKYHT
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            13976..14590
                     /locus_tag="JD016_07570"
                     /pseudo
     CDS             13976..14590
                     /locus_tag="JD016_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088348.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            complement(14539..14847)
                     /locus_tag="JD016_07575"
     CDS             complement(14539..14847)
                     /locus_tag="JD016_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999497.1"
                     /translation="MNFLKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIEKYKT
                     SYKQLLVNPNIEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
     gene            15651..19793
                     /locus_tag="JD016_07580"
     CDS             15651..19793
                     /locus_tag="JD016_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HAU4999498.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDAVVESIPN
                     PNPTNNIANIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVNFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGN
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVASA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSTTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQIVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENVQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNI
                     AEVTSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDF
                     WC"
BASE COUNT         7275 a         3031 c         3773 g         5872 t
ORIGIN      
        1 gctggaagtg ccgttgctaa taaacgagct gaaagaaaaa atacagcttc taaagaaaat
       61 cggggcaata ccacttccag catgggacag cgtgcaggct caaaggtggg tgctgtctta
      121 gatacgaaaa ataaagtgaa agacaaagca aacgctgtca aagaaaacat caaggatatg
      181 ccgacacaga ccgcttatgc ggtgtattcc gcaaaggaaa aggcaaagtc cagcgtgtcc
      241 gacttcaagc gtggcatggt gcgggaacag cagtccagac agacgggacg attggaaaag
      301 caggaacagc acaagaaaaa tatcgctgac aagcgtatgg agcttcaaaa ggcacaagaa
      361 gcaaggcagg cacagcgaaa ggctgacgga tcagcgacaa caggagctac ccgtccccat
      421 gagcgaccag ccacagcttc aaagccgagt gcggaaaaaa tgcaggaagt caaacgtcct
      481 gccacagcga ccacttcaaa agcaagtgag ccagtcaaga caaatgttat caaagagcgt
      541 ccgttatctt ctggtgcttc tgataggaaa gcgacccagt ctgcacagcc agtacatagg
      601 cagaatgtag aaaaagtggt atcgcaggaa acacgccaga attaccccaa agaccgcagg
      661 acaaaggttc agcagacgca gagcgtccag aagaaccagc agacaacaga gaaaacccgt
      721 aaccttgtaa cgaagaaagg acagaagaaa aaatgaaact gaaacatatc gctatcattg
      781 gcagtctgtt tcctatcctc ttttctctgg tgcttttctt tggagtgttg attagtgcgg
      841 acagcgacga cgagaacagc aatttttctt ctggcattac gggtatgaac ctatccgcag
      901 aagtcttgaa acatcagcct atggtagaaa aatacgccag agaatacggc atttccgagt
      961 atgtcaatgt gctattggct atcattcaag tagaaagtgg cggtacggca gaagatgtta
     1021 tgcagagttc ggaaagtctt ggtttaccgc ctaattcctt agatacggaa agttcaatca
     1081 agcaggggtg taagtatttt gcgtccctgc tttcttcctg caaaaatcaa ggtatcgacg
     1141 atttgaatgt agcgatacag tcctataact atggcggtgg ctatgtgggt tatgtggcag
     1201 gaaaaggaaa gaaacacact tttaatcttg cagagagctt cgctcgtgag aagtcgggtg
     1261 gaaagaaagt aacctacacc aacccgatag ctgttgcgaa gaatgggggc tggcggtatg
     1321 gctatggaaa tatgttctat gtggaattag tcaatcaata tctgactgtg gcacattttg
     1381 ataatgcgac ggcacaagcg attatgaatg aagcgttgaa atatcaaggc tggaagtatg
     1441 tgtatggtgg cagtaacccg aacacttcct ttgattgtag cggacttgtg caatggtgct
     1501 atggaaaagc tggtatctcc ttaccgagaa cagcacaagc acagtatgac gctacccaac
     1561 atcttccact ctcgcaggca aaggctgggg acttggtgtt tttccattct acctataacg
     1621 ctggttcgta tgtaacgcac gtcggcattt atgtaggaaa taatcagatg taccatgcag
     1681 gcgacccgat aggatatgca gacctaagca gtagttactg gcaacagcac ctaatcggtg
     1741 caggacgagt aaaacaatag aaaggacttg aaaaatatgt ttaagaagaa taagaaacag
     1801 acagaaactc tcaaagaacc aaaagaaaaa aaggtgcgta ctgttaaggt aggcacacat
     1861 aagaaaaccg tgattgcgtt gtgggtggtg cttatcgcaa gcgtgagttt tggggtgtat
     1921 aagaatttta ccgctatcga ccagcacacc acccatgaaa aagaaatcat tgagcttcgc
     1981 ttgcaggata ccaacgggat agaaaatttc gtaaagaatt ttgcgaagtc ctactacaca
     2041 tggaataaca gtaaagaagc gattgaagca aggacacagg caatcaacgg ttatctgaca
     2101 aaggaattgc aggacttgaa tgtggatacc attagaaccg atataccaac cagctccacg
     2161 gttacagatg tgattgtatg gagtatcgaa cagtcgggaa cggacacttt ttctgctacc
     2221 tacgaagtag atcagcagat aaaagaggga gaacagacaa gcaatgtgaa agcaacctat
     2281 accgtaaaag tccatgtgga cgctgacggg gatatggtaa tcgttcagaa ccctaccctt
     2341 gcaccagcaa tcgaaaaatc ggactatgag cctaagacac cagaagcaga tgcaagcgta
     2401 gacgctgata ctgtcaatga cgctacctct tttctggaaa cattctttaa gctgtaccca
     2461 acagccacag aaaaagaact tgcctattat gtaacgggaa atgtgcttga acctatcggc
     2521 agggactatc tttattctga actggtaaac cctatcttta caaaggacgg ggacaatgtg
     2581 aaagttaagg ttgctgtaaa attccttgat aatcagacaa aggcaacaca agtatcgcag
     2641 tatgaacttg tactacataa ggatagcaac tggaagatta taggataaat caagaggctt
     2701 gtagttagca tttttgcatg attacaagcc taaacttatt gacaatcgta tcatataata
     2761 ctatactgta cttgtaaagg taatacagta taagacaagg cggtggtata gtggatattt
     2821 ttatcaataa tcatgccagc ataccgattt atgagcaaat ttcttcacaa ataaaggcaa
     2881 tgattatcaa cggagatttg aaagctggag aagcacttcc ctctataaga ggactggcaa
     2941 actcgttgca tattagtgtt ttaacggtgc aaaaatctta tgatgtactt caaaaggacg
     3001 gttttattga aacaactgct ggaaaaggtt gctttgtatc cgttcaaaat caagatttct
     3061 acttagaaga acaacaaaag aaaatagaaa attattttat agaagcgatt gaaattgcaa
     3121 aggtaagcgg aataaagctg gataaattac aatctttact tgcactatta tatgaggagg
     3181 aatgagatta tggaaaaata tgctttggaa cttaatggag taactaaaca atatagtgac
     3241 ttcaaacttg acaatatttc ctttactgtt ccgcaaggta gtatcgttgg attgattgga
     3301 gaaaacggag ctgggaaaag cacaacaatt aaagctattt taggtttgat tgttactgag
     3361 caagggaaaa tttctgtatt taatgggaaa gatgtcaacg ctgaaacgaa agaacatatc
     3421 ggtgttgtat ttgatggaag taattatccc gataatttaa cacccaaaaa gttaggaaaa
     3481 gtttttcaaa atatttatgc tgaatggaat aatgaaaaat attttgagct attaaataag
     3541 tttgaacttc ctatcaataa aaaattaaaa gaattttcaa aaggaatgaa catgaagtat
     3601 tccttagtgg ttactctatc tcataattca aaattgctta ttcttgatga agcaacaagt
     3661 gggcttgacc ctgttatacg agatgatatt ttagaaatgt ttttagattt tattcaagat
     3721 gaagaacatt ccattttggt ttcatcacac atcacaagtg atttagaaaa aatcgctgat
     3781 tatatcgtat ttatacatga ggggaaagta ttattttcaa gacctaaaga tgaattgact
     3841 gagcaatacg gtatcatcaa atgcaatgct aaacaatttg atgaattgaa taaaaatgat
     3901 attatccgtt ataagaaaca agattatgaa tggcaagtgc ttgttgcaaa tagacaaaaa
     3961 gcaaaaaagg aatatccaca tactttagtt gtacctgctt caatagacga tatcatgtta
     4021 ctttatgtga aaggggaatg ttgattatga aaggcttatt attaaaagat tattttgcta
     4081 ttaaatcttc gatgataata ttactcattg gctttgttgt ctttgggttc ggagtttctt
     4141 tttatgctcc accctatgtt ttaatgctga tagcttctat tatactaggg caatttgtaa
     4201 caactacaat ttattcagac agaaaatgtg gttggttgaa atcatcaata acatttccaa
     4261 catcaaaaaa atcacatgtt agcagtaaat atattatgta tgctctatta tgtttgttag
     4321 gggttgttgt tggtctagtt ttaagtatta ttattacttc aatccgtaat gaactagata
     4381 cacatttgct tttattattt acttgtatga caactactat gatatttatg tcaggggctg
     4441 ttcttattcc ttgttactat tttatcaaag aagaattaag cggagctatt ggaattttag
     4501 catacggaat atcgggtgta tttttctttg gcatgattaa gttaattgaa aatcatgttt
     4561 tggcacttac tattagcatt tttacagcga taatactttt tgttatttct tggtgctttc
     4621 tttgccaaca gataataaac agagatattt aggagtgatt taatgctaag aataaaaaaa
     4681 cgagatatta aaaaagcgtt aaaagcaggt gcaaaggctg gcggtatgtc attagctgat
     4741 gttcgggaaa atattgaagc aacgattgat gaagctatga atagcaccga cccagaggtg
     4801 caggcaaatt tcaaaaagta ttttgggaat aaacgcccta caccagaaga atatatttat
     4861 atcattacga aaaagacaaa agtaaaagtg taaataaaaa cgccgtggga aacaactcac
     4921 agcgtgagat ctgattatca cggcgttaaa ttatttttgg agcaaatagt tttctcccca
     4981 gttgcatagt tcatcaagaa taggggttaa ggtttttcca aattcagtta aagaatattc
     5041 tgtttttggc ggtacaactg ggtaaacttt gcggttaatc aatccgtctt tttctagctc
     5101 acggagttgt tgtgctaaca ttttatctgt tgctttggga atgagcttat gaatttcgtt
     5161 ataccgtaat gttttattca ttaaatgcca caaaatgaca gccttatatt ttccaccgat
     5221 tagttgaatg gttgcttcaa ctggacaact aaaaccacag caattatttt ccataatgtt
     5281 ctctcgcttt tcttttgggt agtatattac aaaatagtgc gttcttgtct aatagactat
     5341 tacgaatata attatagtat aggttgacct aattcacaag aacaaaggag attgaaacat
     5401 gaatgtatta gatattgcaa aaaagaggta ttccgttaga acatacgaag ataaaccagt
     5461 agaaaaggaa aagttggaga aaatacttgc ttctgctcat gttgcaccaa cagcggcgaa
     5521 cttacagcca gttaaattat tggtggtgca ggacaaagaa cgattgaaaa gtattgcaaa
     5581 agctggaaac atatataatg ccccattggc aattattgtt tgtgcagacc attcaaaagc
     5641 atggacacga ccttttgata acaaacagac aacagatatt gacgctacta ttttgacaga
     5701 ccacatgatg ttgcaggcaa cagagttggg attaggtaca gtttgggtat gcttttttaa
     5761 accaaatgta atcaaacgag aatttcaatt accagataat ttagaaccag tcaatatatt
     5821 agtcattggt tatgggaaag aaacaccagc agacccagaa cgtcattttg ctacacgtat
     5881 tcctatggaa gaattggttt cttacgacta tttataaaag gtttgtaatt gtaatttttt
     5941 agacaatgaa agtaacgggt cgtgaaaatg cttcacagaa ttgtgatctg cattatcacg
     6001 gctcttttac tctctgttta ctttcaaaac tgtaagtgta attgaatact gcccttgcta
     6061 taatttaatc gtattccaaa acaaataaat gtggcaggaa gaaaggagcg attttaatgg
     6121 aacacatttt aaatttagaa caagtaaaaa aatactacgg gggtaactct ggaaatatca
     6181 caaaggcagt agacggtatt tctatgtatg tagacaaggg cgaatttgtc gcaataatgg
     6241 gagctagtgg ttctggaaag actaccctat taaactgtat ctctacgatt gataccgtaa
     6301 ccagcggaca cattacagtc aacaatcaag atattaccaa aatcaaagat aaagattttg
     6361 ctgatttcag acgtgaaaat ctagggttca tttttcaaga tttcaacctt ttagatacct
     6421 taacgattga agaaaacatt tctttgtcat tggtaatcaa caaacagaac cctgctgata
     6481 ttgacaaacg agttcatgct atcgctgata aattgggtat tcgtgatatt ttatcgaaat
     6541 ttccttatga agtttctggc ggacaaaaac agcgttgtgc ttgtgcaaga gccttaatca
     6601 atgaaccgaa actgatatta gctgatgaac cgacgggagc tttggactca aaatcttcca
     6661 gattgttact ggaaacaatg tctgacatca acaagaaaat gcaggctact attttaatgg
     6721 taacacatga cccgtttagt gcgtcttttt gtaaacgtat tttgttcttg aaagacggta
     6781 aaatattcaa tgaaattttc agaggggaaa aaagcagaaa agatttcttc aatgaaatac
     6841 tggatatatt aaccttgctc ggaggtgaag tgggaaatgt taggtaaatt agcggttaga
     6901 aatacaaaaa gaagcattaa agattatttg atttatttga taactgttac tatcgcattt
     6961 tctcttatat ttgcttttaa cttagtggca agttctgaag aagtaataga attatcgaca
     7021 ggcatggata cgtttaagta tattctagct tttgtgaatg ttgttattat atttgttatc
     7081 tgttttttga ttaactacac cacaaaattt atgtttgaaa agcgaagcaa agagcttgga
     7141 acatatatgc ttcttggaat tagaaaaaaa gaaattgctc gattgcttgt aattgaaaat
     7201 gtgttattag cgatatgtgc tttggttttg gctattcctc ttgggttcat atttagtcaa
     7261 tttgtatcat tggtaattgt aaacttatta ggtattccag aagtaatctt tatttcaata
     7321 aattttgttt ccataggatt gcttgctatt tactttttgg caatatacgt attggttctg
     7381 cttaatcttt tacgaagaat tagaaaaatg acggtacatg attttcttta ttttgataag
     7441 cagaatgaaa agaaaatgtt ccgtagcaat aaaaagagaa atattatttt cattgttagt
     7501 gttatattag gaattactgc tattttgctt tgggcttcac gttggacaat ggaaaacagc
     7561 ggtaaacaag aaactcttac gtatttgatg attagcatga tgatgttgat tatcagtatt
     7621 tatggtgtat cagctacttg tgcagatatg ttgttgtcgg ttttattgaa aaataaaaaa
     7681 cttaaatatc aaaaagataa cctttttgta acaagaactt ttgcgtcaaa agcaagaaca
     7741 atgagtttta cgttcgggac actttccatg ttgatattac tttctttgtt atgtttaaat
     7801 ttttcaagta tcaataaagg agtgtataaa gcaagcatag aaatgacagc tccgtttgat
     7861 gttcatgtgt ttgaagaaaa acaaccattt aaagatttta aagaatatgt gaatgttgtt
     7921 gatgaagatt atacaataaa taaagcaatc gaatttgatg tttataaaga accgaaacat
     7981 caaatgcaga attattttga cgtgcaattt tatgattatg acccagtaat gaaattaagt
     8041 gattataacg aaatactgaa attaaagaat atggacacca tagaattaag taatgacgag
     8101 tattttcttg taactagcaa agatttgtta tataaagttg aaaacaacaa ggatattgaa
     8161 aaaattcagt tgacaagtgg aaaagaatta aaactaaagg gcattgatac aaaaacgtat
     8221 tggtatcaaa taaataatac tggaagattt gctgttattg tgcctgatga atatgttcaa
     8281 ggcttggaag tatctgagca acatctgatt atagatacca tagaagaaac agatacaaaa
     8341 cttagagaaa aaatcaaaca agatttgaaa catcgattag taatagtaaa tgatgatggt
     8401 gaaacagtcg tacaatatta tagactttct gtaagaggtt ctgctattga agaacaaaat
     8461 acgatgaccg ctatgattgc aagcatttgt ctttatattg cctttatttt gatttcggca
     8521 gttggaacag ttcttgctat tcaatcacta agtgactcta caaagtacaa ataccgctat
     8581 caaacattaa aaagattggg agtgaatgat aaatcattat ttaaaacaat cagaaagcaa
     8641 ttactaatat tatttggtgt tccagttatt tattcaattc ttgcaagttt ctttatgctg
     8701 gtatcagtca ataatgttta taaaatttat ttagaaagtg agtatagtta tttaatttac
     8761 ttcgtagtag gtttggcgat tttcttcttt atctatggaa tttattggat agcaacgtat
     8821 atcggtttca aacgaaatat caatgaggag agttagattt ttctagctct cctgctgtgg
     8881 aattattata atggtaaatc aagggggtgt ggaaatgaga atagcaatta tcgaagatga
     8941 tgaaatcact cgtctggagc tttctaaact attgaacaca caaggatatg aaacagtctt
     9001 gttgactaac tttgaaaatc tgacagagga attaaagcaa tattgcgtag agctggtttt
     9061 gcttgatatt aacctgccgt atgaaaatgg ctatgaagta tgcaggaaaa taaaacaagc
     9121 tatgccagta cctattatct ttgtaacaag cagagataca aatgctgacg aattgaaaag
     9181 tattcaagtg ggtggaattg actttatcac aaagccgtat gacacgctta ttcttcttga
     9241 gaagatcaag cgtgcattgc agttatcaaa cccgaacaat ttccgtgagc ttgtcaaaaa
     9301 agattgtacc cttgatttac atttatccat tttgaagtat caagagaaaa gcattgaact
     9361 gacaagaaat gaatttcgta tcttatacta cttctttatg aatgaagata aagttattag
     9421 caaagaagaa ctattggaaa agctgtggaa cgacaaatat tatttagacg aaaatgtatt
     9481 actggttaat atgacccgtc taaaaaagaa aatgaaagaa atcggtattg ttcatttatt
     9541 ggaaaatata cgaggaaagg gttggaaact gtgagctttt ttacattctt agaagaaaaa
     9601 ataacagaga tattgtttca gcttttcttt cttgccttag tagtatttct cttgattttc
     9661 tacggtgtag atacgctatt tgttgtattg ctggtaattc tgttcatcag catacaaggt
     9721 ttttttcaat ggtgtttgta tcggaagaaa cgtaacgctt cacaacatat tattgattta
     9781 gtagacggac tggaagaaac ctattatatt gcagacgttt taccaaagcc aaaagaattt
     9841 caaaatgaag cctattacta cgcactaaaa aaagcctgca aatctatgaa tgatgaaatc
     9901 agtaaaatca cagaagaaaa gcaggactat caagaatatg tggaaagttt cgcccatgaa
     9961 attaaaatac cgataggggc attgtctttg acgtttgata atacgaaaaa ttatacgtta
    10021 aaaaaagaaa ctgataagat atttcagtta gtagagcaaa tgctctatta tgcaagaagt
    10081 gaaaatacgg aaaaagacta ctttgtaaaa cagttaaatt tagacgaagt gatacataat
    10141 atcattttga aattccgtca ttcacttatg gaaaggaaaa cggttatcaa tatccatgat
    10201 atagagaatg ttgtttatac cgatgaaaaa tggctgacat tcattctatc tcaaatcgtg
    10261 caaaatacga ttaagtattt tgacaagcag gaaaataaac tgacgatata cagtcaagac
    10321 aatgaaacaa atatactgct tgtgatagaa gataatggct gtggaataaa agcgtcagac
    10381 ttatcaagag tatttgaaaa gggttttact ggttcaaata gaaacaaagc aaatgcaacg
    10441 ggcatgggac tgtatttatc gaaaaaatta tctgatagac taggtttgaa attagatatt
    10501 acttctaaag aaacggaata tacaagactg acaattacat tcccaaaagg aacagtccat
    10561 aatttacatt aagcattgaa tagttgctta ttgatgataa aacaaaggaa gtgatacaca
    10621 tggagttata tcttaatgat gtttaccctt atgcacaaca aatatcaaat tatatagaac
    10681 gagaaatttt tcaacagaaa ttaaaagcag gtcaaaagct actttcaata acaaatttaa
    10741 gtaaagaatt aaatgttagt aaacatacaa ttcaattatc ttataaaata ctgagaaaga
    10801 aagagttaat cgtaaaaaaa ggaaatgatt tttttgttac aaaagataac tgctgtataa
    10861 taacaaaaat gaaagaagaa acagatttac agataatcaa atgtctgcat gaattagcag
    10921 aaatgggata ttcaagagaa caaacaaaat ccttgatttt aagaaaatta gaaactttaa
    10981 aatttcctca ataagttcga ttaggcattg catagtattt tgctgtaagg gggcttgagt
    11041 tatggaatgt tttaagtgca attctttaca atttaattcc acctaacagt acactattaa
    11101 aaaataaata ttatttagct ttaaaagctc gtacagacta tatgttttgt gcgggctttt
    11161 ttcatacccg aaaaaaactt aaaaattttt caaaagaggt cattaaatca cacctagctg
    11221 tccttatatg gtgcggaaag agggataaac acttttcaaa tcataaagga aaggaggtga
    11281 gattgatgaa accgtctgac ttccagaaaa cagttcaatg tcgttttgaa agttgtttaa
    11341 agaaagttgt tcgtagtgtc gtgaaagatt attacaagga attaaaccgt cgtaaaaata
    11401 aagaaatatc tttcagcgaa ttgccagatg tcattgttga taaaatgact gtttgggacg
    11461 actacgaaac agattataca atcttttcag tatgtggcat tgatattcgt gtccttgatg
    11521 atgaattggc agaagcattg aagaaacttc cagaaagaaa acgcaatact ctgttgatgt
    11581 attacttttt ggaaatgaca gaaagtgaaa ttgccaactt gcagaaaatc acgcaaagcg
    11641 gtgtatttag aaatcgacat cacgctttgg aaattatgaa aaaaatactc aaggaggaac
    11701 attagaaaat gaaacagtta tttaaaaagc cgtcctatta cttgatttca tcagctatgg
    11761 acggaaacga aaaagcgatt gagaaattgc tggcatttta tgacccgtac atatcaaagt
    11821 gctgtttacg ttcactttat gacgaatatg gcaatgttta tattgttgta gacatggaat
    11881 taaaaggacg tatcagagaa gcacttatca aaatgatttt aggttttaat attgaagata
    11941 tggacttgga accagaaaaa taagcagttt ttagcggagc atgatttaat gtccccctct
    12001 ttcctgctct gcccctgctt ctacatgaaa gcaacacgct ttcgctacag ctctttgaca
    12061 actgaataaa gcagacagat acgtttgtga gatagcgagc cacgggaact gtacgccacg
    12121 accttttaaa agaaagcgag cgacccacgc agtgatccga gagcgactgt tggaaaagtc
    12181 gttttgccac gacctatcct cacagaataa tgatacgtcc gcatggtgcg gttctgccca
    12241 caagaatggg aatagttgag atactaacgg agttttccaa agccgtctgc ctgcttgtaa
    12301 aaaatgacac acagtaaagg gggtgcatag attatgaaca atactgatgt gcctatctgg
    12361 gaaaaatata cgctgacgat tgaagaagcg tctaaatact tccgcattgg cgaaaagaaa
    12421 ttgcgtaaat tagccgaaga aaatcttgac gctggctggg ttatcgtgaa cggcaaccgt
    12481 attcagatta agcgaaaaca atttgaaaaa atcatagata cattggacga aatctaatgt
    12541 cattgagccg tagatatggt ataataaggt atagcgtatc acggctcatt ccatgacgga
    12601 aaggagcttt acactatgtc taatgtaaaa cgaaaagaca gtaaaaatcg caatttgcgt
    12661 aatggagaga gccagcgaaa agacggaaga tacgtttata aatataccga tatatacgga
    12721 aagccacaat ttatttattc ttggaaactt gtaccgacag acaagacacc tgctggaaaa
    12781 cgtgatgata tatcgttgag ggaaaaagaa acacagataa aaaaagacct taacgacggt
    12841 atcgacacag caggcggtaa aatgacagtc tgccagctct acgacaagaa gaacagccaa
    12901 agaaagaaca tcaaaagggc tactgaaaaa ggacgacagt atcttatgaa cgctctgaaa
    12961 aatgacccgt tgggtatgag agcgattgat accgtcaagc agtcggacgc taaagaatgg
    13021 gctatcagaa tgagtgaaaa aggatatgcc tacaaaacga ttgataacta taagcgttcc
    13081 ttgaaagcgt cattttacat ggcgatacaa gacgactgta tcagaaagaa cccgtttgaa
    13141 tttaagctaa gtgatgttct ggaagatgat acggagcaga aagttatcct tacaccagag
    13201 caggaagaac gcctgcttgc ctttatggaa acggataaga tttacagcaa gtattatgat
    13261 gagattgtgc ttctgctgga aacgggactt cgtatttctg aattttgcgg actgacgacg
    13321 catattgata tgcagaacag aatactcaat atagaccatc agttattgaa agatagcgaa
    13381 atcggctact atattgaaac gccaaagacc aaaaacggaa aacgggaact tccattgaca
    13441 gaacgggctt atcaagcaat ccaaagaata ctaaagaaca gaggaaaagc acaaccgctg
    13501 attgtaggtg gttacagcaa ttttctattc ttaaaccgtg agggcttgcc taaagttgca
    13561 ggaaactatg agggcatggt gcgaggactg attaagaagt ataacaagta tcacacggac
    13621 aagttaccga acatcacacc acattcattc cgacatactt attgtacgaa tatggcaaac
    13681 aaaggaatga accctaacac tctgcaatat ctcatgggac acgctaacat aaccatgaca
    13741 cttggctatt atgcacacgg tacatttcaa tctgcaaaag cagaactgga aagactggct
    13801 tgttaatatc ggagcttata tttactactc atttactact tttggttgca ttttcatgct
    13861 ggtaaatgcc agcttatgca aggtatcttc caaaaagaaa ataccgataa agccctaaaa
    13921 tacgggatat atcggcattt accaacttat gaaaagataa tcagaaattt agtaagtttt
    13981 taagaaaaaa attaaagaaa agaaggtttt ttatagggtg ttaaaaataa taatcagcat
    14041 ttttttaata gtatttgtag ttactgagag tctaattatt ttttatccca aaaatagttt
    14101 agaatcaaaa agtgattatc tcttaatttt aggagcttct gtaaaaaaga ctacaccaag
    14161 tacaaccctg aagggtaggt tagacactgc attgaaatac ctaaaaatta atgataattg
    14221 ttatgtggta gtatctggtg gtaaaggaag tggagaaaaa ataactgagg caaaagctat
    14281 gaaagattat cttataaaaa atggtataga taaaaatagg ataatagaag aggataaatc
    14341 cacgaatact tatgagaatt ttaaatattc aaaattgaag atagaagaac atagtcaaag
    14401 aaaactaagc gatttaaaag taaaaatagt aacaacagat tttcatgttc tcagaagtaa
    14461 aatattagca tatagaaatg gatataaaaa tacgtctttt tatgctagta aatcaaaact
    14521 ttcatttgta ccaacttact atacaagaga attttttgca ttatggaaaa caatcatttt
    14581 tgatagataa taaataaaaa gactagatat aaactcatat ctagtctttt ctattccctt
    14641 aattctaatc cattttccaa ctttacttgt tgtagaaatt tcaatattgg gatttacaag
    14701 taattgctta taagaagttt tatatttttc gattccaaaa tataattttc cttcataatc
    14761 cattataaat ccaaatggtc ttactcttgg tttattatct tcaactgtag caaaatagaa
    14821 tgttgagttt tcttttaaaa aattcattat tttttcatga ttaaatcctc tttgtatctt
    14881 atactaatat aatgaatata aataaaatat tcattatact taatataaat cgatataaaa
    14941 acaattacaa tattatattg attattcaaa attatatata aaattcagaa aaaaaattta
    15001 gtttacataa taaaaataaa gtcaactaaa attattgatt ttagattaaa gtaccagatt
    15061 aagcatttaa gtagatttta agcttgattt gattagaaag gtacaaacta tcaccgaaaa
    15121 aaattaaacc catttaaaaa gcaatataaa agcttgttat ttttgagaaa aatagaccct
    15181 ctaaactcaa ttagataaaa ataaaataca gaaaattata tctctaaata tctcaagtaa
    15241 ttaattttaa attttacgat attttgtttg atgatttata atataaaagc atactcttaa
    15301 tctgctcaaa ttaaaaagaa taaaaaataa caaaaaaaat cactgacatt tcactgacaa
    15361 atggtaattt tgttattata aatacattga aaatacttga ttattgaaaa atggcataaa
    15421 ataatacctt ttatgccatt tactattatt tgatagtttt taacttatga cataagatat
    15481 tttttaacat atacaataaa atataaaaaa attgatattg caaataaaaa aaatacatat
    15541 tagatgttat agaaaaaaag cgcacttata ggcttaaaat gtcacataaa agagttttaa
    15601 agaataatat attatgaaca caaattttgt aatcacaaag gagtgataat atggcaacag
    15661 tatcaggaca aatagttttt gatagaaata gaagtgctac cattgattct ggagactcag
    15721 gaatagccaa tataccagtt gtattacaag atatagcaac tggagttaga cttgtggttc
    15781 tcacagatgc atcaggaaat tatgttttta ctaatgttcc aaatggtaac tatagaattg
    15841 tagaagcttt tggaactaca ggaggagtac caacaccagg gaattttgca acagcgatag
    15901 taggtccagt tccaattgca gaaatgccac ctattagttc tgtgacaaat cctcctattg
    15961 gttcaacaaa cttagatggt acaacaccta acacactatt aataacagta gcaggttcag
    16021 atataacaaa tcaaaatata ttaaatgggc ctgtgattta tacacctata gagaatatat
    16081 tggattcttg cgtatctgta tctaatataa accttattac agatgcagat aacggaacat
    16141 tcggtttttt tccaccaggt acaccaagta atacaggacc tgaaatagaa ccttacccaa
    16201 atgtgacgcc agattttacg tatgttgtac cagacccaac taagtttaca cctttagatg
    16261 gagaatatac agtacaaaat attatgacaa atgcaatgag taatgttata ggagcttggt
    16321 ggcgtattgc tgaccatact acaggaaatg aaacaggaag aatgatggtg gtaaatggtt
    16381 ttaatcctgg agctgtattt tttaaatcaa cagtacaagt tacacctaac actaattatt
    16441 tatttagcac ttggatttta aaccttttta aggtaacagg atatccacca ccacagctag
    16501 gtgtaagaat atttgaccaa aataataata tactatatca agcaacatta ggagctcaaa
    16561 ttccagttaa tacaaacgca ccagaatgga aagaagtagg tactgtgatt aattctcaaa
    16621 ataataatca acttacagta gaatttttta gtgaaggaga aagcgccatt ggaaatgact
    16681 atgctataga tgatgtttct ttaagagaaa tagctgttcc aacttttact ccaataaaaa
    16741 caagtaacac agaaactgct aatgtaggtg atataatcac atatacagtt acattagata
    16801 atacttgtga tagcccttta actaatgtat tttttaggga tttaattcca aatggtttag
    16861 agtttatacc tggaactgta acggttgatg gagtatctgt gccaggagta gaccctaata
    16921 caggtttccc acttccagat attggaggag gaacaggggt tgaagtaact tttgatgctg
    16981 tggttgaaag tattcctaac ccaaatccta ctaataatat agctaatata gattactcct
    17041 atacacctgt agaaggcggt ataccaaatg atttttcagt agattctaat ccagttcctg
    17101 ttgaagtaat atcagcagat attgaagtta caaaattatc tgaacctact atagtgaatc
    17161 caggagagga attaatatac actataaaag tagtaaataa tggaccattc ccatctgaaa
    17221 atgtggtttt aacagatgat gtaccagctt ctattataaa tccagagtat tcattagatg
    17281 gaggagtaaa ttttcaacca tggactggaa gtttaaatat agggacatta gaagtaggag
    17341 agacaagagt tattataatt aggggtattg taaatccatc aacagtagga attataacaa
    17401 atacagctgt agtatcttca actacagcag acccaaacct taataataat acatctacaa
    17461 tagagacaga agtgaattta ttagcagata ttttagtaat gaaaacagca gaacctaatt
    17521 cagctgttcc aggtacttta ttgagatata ctattcaagt agaaaactta ggaccagcaa
    17581 atgctgaaaa tgtaatatta aatgatgata ttccagcttc tattataaat ccagaatatt
    17641 cattggatgg aggagcaagt tttcaaccat ggaatggaag tcttaatata ggaaatttaa
    17701 actctggaat ttcattaaca gttttaattc aaggaacagt tagtttaaat tcttcagaat
    17761 atatagtaaa tacagcaaca gtttcttcaa ctacacctga cccagattta agcaacaata
    17821 tatctaccat tattacacca gttaacccac aagcaggtat ttctattata aaagtagctg
    17881 acgaggatgt agctgttcca ggagaagaat ttgtatatac aattgaaatt tttaatgaag
    17941 gaccatctaa tgctacgaat gtagttttaa cagatgatat accagatgtt attttaaatc
    18001 cagaatattc attggatgga ggagcaactt tccaaccatg gactggaagt cttaatatag
    18061 gtacggttgc accagggcaa ttaataagaa ttatcataaa gggattagta agttcaactg
    18121 ctacaggaga tattactaat acagcagaag taagtgcaga tgtaccagaa ccagttacgg
    18181 attcatcaac agtgacaact cctatagttg catcagcaga tatagaagta ataaaaacta
    18241 gcaatatgga tacagcagtt ccaggagaaa cttttagtta tactataaca gttacaaatt
    18301 taggaccatc tgctgcacaa agtatcgttt tgacagatga tataccagat gttattttaa
    18361 atccagagta ttcattggat ggaggagtaa ctttccaacc atggaatgga aatcttagta
    18421 taggcacttt ggatgctgga gaaataagaa gtattataat aagaggaaca gtgagccaga
    18481 cagcagtagg tacaattata aatacagcaa caataagttc aactacacct gacccaaatc
    18541 cagataataa tacttcaaca gatgaaacag atataagtcc attagcagat atttctgtga
    18601 taaaaggcaa cgaaccagtt gctataccag ggggaagatt tatttatgga attgaaattg
    18661 caaatgcagg accatctttt gcagagaacg taactttaac agataatata ccagcttcta
    18721 ttttaaatcc agaatactca atagataatg gagtgacttt ccaaccatgg aatggaagcc
    18781 ttaatatagg tactttagat gctggagaaa taagaaatat cataataagg ggaacagtaa
    18841 gtcaaacagc aataggcaca attataaata cagcaacagt aagttcaact acacctgacc
    18901 caaatctaaa taacaatact tcaacatcag aagcagaggt aagttcatca gcagatattt
    18961 ctgtagtaaa acgaagcaat cagattgttg ttgtaccagg agatgtacta gactatacaa
    19021 ttgaggtgag aaatgcagga ccgtctactg ctcagaacgt aactttaaca gataatatac
    19081 cagcttctat tttaagtcca gaatattcaa tagataatgg agtgactttt caaccatgga
    19141 atggaagtct tagtataggc actttagatg ctggagagat aagaaatatt ataataaggg
    19201 gaatagtaag tcaaacagca ataggtacaa ttataaatac agcaacagta agttcaacta
    19261 cacctgaccc aaatccagat aataatactt caacatcaga aatagatata agttcatcag
    19321 cagatatttc tgtaataaaa ttagcaaata aaacagaagc atgtgtagga gagcaaatag
    19381 attttgtaat agtagtatca aatgcaggac cagaaaatgt tcaaaatgtt actttaatag
    19441 ataatgtttc agataagctt aagaaagcag tattttcact tgatagaggt gtaagttttc
    19501 aaccatggac tggaagtctt aatataggta cactaccagc tggaacttta agagtgatta
    19561 tattaagagg aattataaaa tcaacatgtc ttgatagatt aacaaatatt gcagaagtta
    19621 cttcaacaac accagattca aaccttaaca ataatatatc aagagtgcaa gtagaaataa
    19681 aacaatgttg ttgtaatgat tgctgttgta acaactgtag taattgttgt tgcaatgatt
    19741 gtggttgtaa agattgttgc tgtaattgtt gtaaaaatga cttttggtgt tgattttata
    19801 aaataatttt gtatacctta aattaaactg aaataaaaaa taaattcaca tatgagtaaa
    19861 atattttata aatattaata aattttctat ctttgatatg gaccacattc gtaatactag
    19921 ttattaaatg aagtggtcct ttttaattca t
//
LOCUS       DACXMZ010000054        19942 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.054, whole genome shotgun sequence.
ACCESSION   DACXMZ010000054 DACXMZ010000000
VERSION     DACXMZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.054"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(87..1691)
                     /locus_tag="JD016_07585"
     CDS             complement(87..1691)
                     /locus_tag="JD016_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAU4999499.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVVDNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            2078..3004
                     /locus_tag="JD016_07590"
     CDS             2078..3004
                     /locus_tag="JD016_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAU4999500.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(3124..4122)
                     /locus_tag="JD016_07595"
     CDS             complement(3124..4122)
                     /locus_tag="JD016_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HAU4999501.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            4226..4663
                     /locus_tag="JD016_07600"
     CDS             4226..4663
                     /locus_tag="JD016_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAU4999502.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            4749..5336
                     /locus_tag="JD016_07605"
     CDS             4749..5336
                     /locus_tag="JD016_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HAU4999503.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(5636..6256)
                     /locus_tag="JD016_07610"
     CDS             complement(5636..6256)
                     /locus_tag="JD016_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HAU4999504.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(6264..7031)
                     /locus_tag="JD016_07615"
     CDS             complement(6264..7031)
                     /locus_tag="JD016_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HAU4999505.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGAIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(7072..7893)
                     /locus_tag="JD016_07620"
     CDS             complement(7072..7893)
                     /locus_tag="JD016_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999506.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(8252..10162)
                     /gene="phnW"
                     /locus_tag="JD016_07625"
     CDS             complement(8252..10162)
                     /gene="phnW"
                     /locus_tag="JD016_07625"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAU4999507.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRENGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(10441..11226)
                     /locus_tag="JD016_07630"
     CDS             complement(10441..11226)
                     /locus_tag="JD016_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAU4999508.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(11443..11673)
                     /gene="dltC"
                     /locus_tag="JD016_07635"
     CDS             complement(11443..11673)
                     /gene="dltC"
                     /locus_tag="JD016_07635"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAU4999509.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(11692..12840)
                     /gene="dltB"
                     /locus_tag="JD016_07640"
     CDS             complement(11692..12840)
                     /gene="dltB"
                     /locus_tag="JD016_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAU4999510.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     VSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMVTMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(12840..14354)
                     /locus_tag="JD016_07645"
     CDS             complement(12840..14354)
                     /locus_tag="JD016_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HAU4999511.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(14475..15665)
                     /gene="dltD"
                     /locus_tag="JD016_07650"
     CDS             complement(14475..15665)
                     /gene="dltD"
                     /locus_tag="JD016_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAU4999512.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(15953..16972)
                     /locus_tag="JD016_07655"
     CDS             complement(15953..16972)
                     /locus_tag="JD016_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAU4999513.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(17148..17687)
                     /locus_tag="JD016_07660"
     CDS             complement(17148..17687)
                     /locus_tag="JD016_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HAU4999514.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(17788..18468)
                     /locus_tag="JD016_07665"
     CDS             complement(17788..18468)
                     /locus_tag="JD016_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAU4999515.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(18497..19786)
                     /locus_tag="JD016_07670"
     CDS             complement(18497..19786)
                     /locus_tag="JD016_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAU4999516.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         6529 a         3476 c         2047 g         7890 t
ORIGIN      
        1 gttgatttaa ctgtaatctt atttaattct acctaactta ttatataaaa ctatttttta
       61 tagattataa aatatctaac tttcatctac ttcaaagaat acaatacttc tccttctctt
      121 atagtctgca atactttaat atcttttatt ttcatagggt cgattgtaag tgggttttca
      181 tctaatatta tcaaattagc taatttccct tctttaattg aacctttttt atcttcttca
      241 aaatattgat atgctgcatt tatagtaaca ccctttaaag catctaatgg gcttatcttt
      301 tcattttctc ctatttgtat accttttttt gtaattctat tgacagcaca ccatactgtt
      361 tccagcatat taggtgctat tactggtgta tcctgatgaa gagtgtaaat taatcccttt
      421 tctatagttg ttttaagtgg gctaatctta aacgctctat cttctccaag gtttttgata
      481 tgaatatcac cccaataata tgtgtgagct acaaagtatg aaggtatcat atttattact
      541 ttcatatcat caatctggtc tgctcttact gtttgagcat gtatcataac tggtcttata
      601 ttattttcac tactttgctc ttttaagttt aaaactttct caaacgcatc tatcaactgg
      661 tctgctgctg catctccatt acaatgagtt aatagttgca tcttttcttt tagtgaaata
      721 tctataaact tttctacttc ttcatctttg tatataggat atccacaata accatcatct
      781 gaattttcat atggtctact caaccatgct gtcttacctt gtggagaacc atctaagaaa
      841 atcttataac ctcctatttt aaatctattt atatattttt ttataaactc cctattatta
      901 tctactacag acttactttt tttcaaattt acatatccta caacatccat ttttaattta
      961 ttttgatttg ccattgcttt taaaatgtta aattgatttt cttccattaa accttcttgt
     1021 gctgttgtaa taccatagct tagatatatg ttctgtgctt tttcaattga attaaaaatc
     1081 tcatttgatg atggttgttt tattttagat gctacattaa aaaatgcatt ttcttctaaa
     1141 tatccatttg gttcttcact gccttctact ctacctatat gtccaccttc tgggtcacgt
     1201 gtttcattgg taacaccaag ctgttttaac cccaatgtat ttgcaactcc catatgacca
     1261 gaagcatgtg aaattagtat tggattttct gaagatgcac tatcgagtac actcttatct
     1321 ggatgcttat tttcttctaa aaaattatta tcatatgaaa atccaattat ccagtctcct
     1381 ttttttatat tattcgactc tttgaaatcc tgtatttttt ttactatgtc tttaaaactg
     1441 gttgcatctt ctaaaggaac tagctttaga gtagtagcaa aagctatcaa gtgactatgt
     1501 gaatctataa aagatggcat caatgtctta ccttgtaaat ctataatttc tgtatcttta
     1561 tttttcaaag caaatacttc ttcttttgtt cctatctttt ttattatttt gtcttttata
     1621 agaatagcat ctccacatat tgaatcttcc attgtgatga tatttccatt gtaataaatt
     1681 tttgctttca taaaattctc cttacgttgt ctacttttaa taaatttttt tcattatttt
     1741 ctgcttaatt atattatctt ctttataaac tttttcttta gagtttaatc atataaaagt
     1801 tttattttta attattgttt gccaagaagt aaatactatt cattgtaaat aagacaaatt
     1861 aataggcttt atactaattg aaaaaatatt ataagttgtt aaatttaaat atctaaactc
     1921 aattatatct gtacaattgt aattttttaa atcatgatgt attattttaa atatacagtt
     1981 acattattat tcacaatata agtatcttag ttctcaaata acatctcatg taatctattc
     2041 aatgcaaaaa atttaaatat tttttaggag gttttttatg agcaaggaaa ataaaaatgt
     2101 aagaattgct gttgtccagg cgtcaccagt cataatggat ttagaaaaaa ctgttgaaaa
     2161 agcattagga ttaattaaag aagctggtag aaaaggggca aatatcgtag ttttccctga
     2221 agcattcata cctgcatatc caagaggtct ttcatttgga tttgttgttg gaagtagaac
     2281 aatggaaggt agagaagatt ggaaaagata ctatgataat tctgtaccag ttcctagtgc
     2341 tacaacagat ttattaggaa aagcagcaca agaagcagga gtatatctat caatgggtat
     2401 aacagaaaga gatggtaatg atataaattg taccctttat tgcacaaact tatttttctc
     2461 tccagaaggt aagctaattg gtaaacacag aaaacttaaa cctacaggaa cagaaagatg
     2521 tatttgggga gaaggagatg gaagtactct tacagtagtt gatactccat atggaaaaat
     2581 gggttcgttg atttgttggg aaaattatat gccattagct agaacagctc tttatgctaa
     2641 gggtgtaaaa ctttacatag cccctactgc agattcaaga gaagagtggc aagcaactat
     2701 gaaacatatt gctttagaag gaagatgttt tgtaattgga tgcaatcaat atgttgaaaa
     2761 aaatatgtat ccaactgacc ttaactacta taaagagcta gatgcagaac cagaaattat
     2821 gtgtccagga ggaagttgta tagttgaccc ttttggaaaa tatgtagcag gtcctatttt
     2881 caataaagaa gaaatgctaa tagctgattt agacctagaa aaaatagtac ttagtagact
     2941 agattttgat tcagaaggtc actattcaag accagatgta tttgaattaa ttgttcatga
     3001 ataatctttt tacacaaaca tctacataaa tctttttaca taaaaaaatg agatactcat
     3061 aagtaaattt caagtatctc atttttgctt aaaacctata atcctaatta taaatttatt
     3121 cacctatatt ttttctaaat caactactgt tcttccaaca tgttttcctg ccattattga
     3181 atctatacta tcatttaact cttccaatga tactacattt accatatcat ctaaatcatc
     3241 taatttccat tcatttgata ataaatccca tatttttaat cttaagttca ttgggcaatc
     3301 cacagaatct ataccatata aagtaattcc ctttaatata aatggatata ctgaacttgt
     3361 aaaatcaact cctcctgcat taccacaagt tgttactgct ccacaataat tagttgtctt
     3421 tattgctgtt gctagagtat ttcctcctac tgtatctatt actccagccc atcttggtct
     3481 aagcagaggt cttcctgagt tatcatccac ttctgaccta tgtataacat ctttaacacc
     3541 taagtttagt aatacttctt tttgttcttc taattttcct gttgaaacta caactgaata
     3601 acctaattta atcaatattt tagttgcaat tacagcaact cctccagtac caccagtgac
     3661 taatacatct cccatattct tgtctacaga atctataaat ttatacacag ataaagctgc
     3721 tgtaaaccct gctgtaccat aatccattgc ttcttttagt gaaagatttt taggaagttt
     3781 aactacccaa ctagaaggaa ctcttatgta ttctgaaaaa ccaccagaag tattcattcc
     3841 caagtcataa cctgttacta aaacttcttc tccaatttta aaatcatcac aactactaga
     3901 ttctactata ccagatgcat caattcctgg tgtatgagga tactttctcg taacaccctt
     3961 attaccattt gcagaaagtg catccttgta atttaaagaa gaatatttta cttttattag
     4021 aacttcgcct tctggcaatg tatctacttc tctttcaact atatttcttc taaaatttcc
     4081 ttcttcttct gtaatatata gtgatttaaa ttttttattc ataaatttcc tcccaataca
     4141 tttagtttta tgttaaacta attatagttt ataaatagtt ttatgtcaaa ctaatttaaa
     4201 tagattaaag attggagaat aatttatgga agataaatat attgtacatt tcataagtaa
     4261 aacaaaggct agtatgatta aattcattga aaataaactc agtaaaaatg gtttaggtga
     4321 gttaatacct actcatggaa atattttaac tgcactttat gaaaataatg gaatacttac
     4381 aatgaaagaa attgcagcta aaataggaaa agataaatcc actgttacag tgttggttaa
     4441 taaacttata aacctaggat atttagaaag acaaaaatgt acaaatgaca aacgtatcac
     4501 gtatataaag cttacaaaaa aggcactgct tatagaagat acttttaata gcatctcaac
     4561 acaagttaaa gagacggcat atcataatat tacagaaaat gaaaaacaag aatttcttag
     4621 aattttaaaa aaaattaatg ataattttaa aaatgctgac taaattgagc ataattatat
     4681 ctcctattgg aataataaat aaaaagaatc aaatattgta aaaatttatt gtgatagaga
     4741 ggtagaatat gaatttaaaa gatagtaaaa ctaaagaaaa tttattaaga gcttttgcag
     4801 gtgagagtca agctagaaat agatacaata tttctgcatc tgtagcaaaa aaagaaggat
     4861 tatatataat agagtcttta tttaattaca ctgctgacca agaaaaatca catgcaaaac
     4921 aattcttttc taaattaaaa gaatttgctg gtgaaaatat aagtatatca ggagcatacc
     4981 ctgtagattt atatgacaag acagcacaac accttaaagc tgcacaacat aatgagcttc
     5041 aagagtggga tgatgtatat aaaaactttg gaaaaacagc acgtgaagaa ggatttgaat
     5101 ctatagctaa gttatttgaa aatatagctg aaatagaaaa agttcatggt gatagatttg
     5161 gtagatatgc tactgattta gaagaaggta ctttattcag aagaaatgaa gatatacaat
     5221 ggatatgtac acattgtggc catatacata taggtaaaga agctccaaaa gcttgtcctg
     5281 tatgtcttca tccacaagga tatttcttag attttaaaaa ctcattattt gaataaatta
     5341 aggcaataat tctatttaaa caatttacta tatttatatc aagtattttt ataaatactg
     5401 tattatctta ataacaataa aatgaaccat tgatattact ataagtagaa aatgtagttt
     5461 aaatacaatt tatagtagtt catcatggtt cattttatta tttactaatc cctataacct
     5521 acttaaatct atctttaact tttacacact cacataaaat ctcatcttta aactaaatat
     5581 ttaaataaag tgtattttaa gtatattatt ataatacatt atataagata atacactatg
     5641 ataactttat acttatatca tttatttctt ttgtcaagtc aacctcaata atcttaacta
     5701 catctttttc aaactttact tttttataat ccaaacatcc tactacgaat ggaactccaa
     5761 actttctaaa caaataaatc tttccacttt cattgtatat tgatgtaaaa ttttcttcta
     5821 ttatattcac tatcaatttt aactcttcaa aagttattat agatttaact aaaaacttct
     5881 ctattatgct caatataagc agttcttttt tacctatgct ttgctctttt ttatataaat
     5941 tttgaaatat ttctttggta taattacata taacttcttt actgagaata aaatcaatgt
     6001 caaattcttt attaatatcc tttttcataa acatatttgc aatatcatca agagaagtct
     6061 cttctttcat agacaatatt aactctattc tttctagtat tttatctctt ggaaagaaag
     6121 tctcttgacc tgtaaaagct gattttttta taaaccattc ctctggtatt atgttttttc
     6181 ttttccatct atataattga ccataagata tattagtttt gctcaataaa tctttcttag
     6241 atattaattc ttccataact tttctagccc acctttatac tgttttttac agtagaatta
     6301 tctgaaactg ataaattatc actgtattca atatttccaa cgtcacaatt atctactaga
     6361 gatatgcttt tgcctcgtat atcttttatt gatgcatctt ctaattcaat agtatctccc
     6421 tctatcaaat tacatttaaa ctttcctgaa aaaatattaa tgattttaat taatgaaaaa
     6481 gcatctttgc ttcttcctat ttttatatta gttgccccta tctctttaca ataagaacct
     6541 cctcctgaat aaacatatat attttctcca tttaaaagac catcacattt tattgctcca
     6601 tataccttta tatcttcaca ttctatattt gatttatttc tcatttcgcc atagattttt
     6661 atatcttgtg catacatatt ttcatttaca cttaattgtc ctcttattgt aacattatta
     6721 catttaagtt tgccatccac tttctgttga gcagttataa ctaagtcatc tatttctgca
     6781 tctccctcta tattaagttg accattcact cttacacgct ctgctttaag attgcctctt
     6841 ataatagttt ttccgttaac aacaaaattt tttatattga tgtcaccttc aaattttgca
     6901 tctccaagga cttttacttc gttactttta acatttcctt ttgatttaga acttcccacg
     6961 atttttatat tttcatactc accactactt ataaagcctt ctccaagtat ttttatagac
     7021 cctttttcca tattattacc tcattttaaa ttttattttt aaacctattt attacttatc
     7081 cttctttaat tttatttttt cacttatatt ctttatttca ccaactattg aatcttcatc
     7141 aatctctata ctttcactgt actccaaact ttctacataa catcccttgc ctattttaat
     7201 attttttcct cttacaactt tgacttcact attttctaag cttatttcat ctgcttctat
     7261 aatatttgca aacaatttac tttctgtata ctttctaatt gataatagac ctaaacttat
     7321 taaatttgaa gacacttttt caaagttgat atgctttttt tctagcatac gaaatttttt
     7381 gcaataatct ccatgcccct tttttatatt tatactactt gctccaactt cattaaaatt
     7441 agaagttcca tttaaactaa tattaacttc ttcacaattc aaaaatccat tacactccaa
     7501 gattccactt aaattgatat tttcacaatc tatattctca tttgctttta aatatccttc
     7561 tccatgaata atatccgatt ttatacttcc ttctacatta ataattccac tagtatcaat
     7621 ttcaacagaa ttaatatttc ctgtacatct aagaagtccc gatatctcaa cttttgatgc
     7681 cagtatgttt ccttctactt tacttaaacc agatgtaaaa aattcattac attctgttgt
     7741 cccaaaaact tttgctgaac cagaagaatc aaatttacta cattttacac ttccttctat
     7801 tttgcctgtt cctgatatat taacattatc aaaaattcca cctttacatt ttccaacacc
     7861 atcaaatctt agtgaactta attcttcatt cataatgata tcctttcttt accgtatttt
     7921 atctttaaac ctaatataac aaaacattgt tacgttgtca agtatgataa taatacttaa
     7981 taaagcaact atcatttatt ttctataaat aggcataaaa ataataggga aattctttat
     8041 ttaaactata aatctaattt cttagattgt ggtttaaata aaatatatcc ctattactca
     8101 atctttatat attcattgta taaacttcta aaatataagt ctttaaattc tactgtcaat
     8161 actttttata cactgtttgt gctatcatct cacacaccta aaggagtggg ctttccgcac
     8221 tattctgtaa cctacaagac actatcttct tctatctatt gataaatttt tctattacat
     8281 cagctagtct ctccatatca gttggataaa cttcacctat gctacctatt ctaaatgtat
     8341 ctatatcagt caatttacct ggatagataa caaatccatt atcttttaaa taagtataaa
     8401 attccataaa ttcaaacttt gcatttttag gatataagaa agttgttatt acaggtgatt
     8461 gagcattatc attcaccaaa gtatcaaatc ctaaagattt taatcttgaa gctattgtaa
     8521 actgattttc tttatatcta gcatatcttt tttcaacaga accttcttct tccaattcta
     8581 ataaagcttg ataaaaagct cttacaacat gtgtaggtga ggtaaatctc catttaccat
     8641 tatttttctc catagtctcc cattgcgcat acacatcaag agataatgat ttagctatac
     8701 ctttacattt agataatttc tctttattag ctattataaa accaaatcca ggtactcctt
     8761 gtatacattt gttagaacta ctaactaaaa aatctatatt aaaatcttca acatcaatct
     8821 caattccccc aaaactactc atagcatcaa ctatataaat tttgttatat tcttttgcaa
     8881 gtttccctac ctcttgtata ggatttaatc tacctgtagt agtttcacag tgaaccattg
     8941 atatatgtgt tatatccttg ttttctttta ataaattttc tactacattt aaatccactg
     9001 cttctatatc tttaaatgtg caatcaacaa attctatgtt aagataatta catatatctt
     9061 tcattctctt tccatatgct ccatttgcaa ttacaagaag tttaccatct tttgaaatag
     9121 tacttcctat tatagcttct actgaaaaag tcccacttcc ttgcattaat acacttgtgt
     9181 atttatctgt attttgggtt gctagagata ctaatcttct tctaacatct tgtactaggt
     9241 tattatattc aacatcccaa gtacaccaat cttttaacat agatgctctt actgatttag
     9301 tagtagatag tggtcctggt gtaagtaata tgtaatcatt ttctggtaca aaatctgatt
     9361 taatagtttc attctctatt tttattagta tatcttctaa ctcagccata gtttctatta
     9421 caaaatgtgc acctgcctct ttaaatttct ttgaaactat actcatctta gcttttaatt
     9481 cttccttatc catgttctct acttcttctt gtgtaagccc taactcactt gaacctttta
     9541 ttacagcaac actccacatt cctgcattta cgccttcttt tacatcactt attgtatctc
     9601 ctacttttac catagaactc ataggagaca cccctaaagc ttcagcattt ttatagcaca
     9661 tccaaggata cggtcttcct tgagatactt ctgatggtgt tacaagaaaa tcgggtgagt
     9721 atcctttctt tgctgcattt ggttcaacta tgttcatcat ctctcttgta taacccgttg
     9781 tagaacctat ttttaatcca ttttctctca acttttcaat agtttcaaca acatgaggaa
     9841 ttggagtcgt atactcttct aatgtctcaa ataacattgg ctcaaattca gcataaagtt
     9901 catttacatc atcttcagtt ggcactttac caaacttatc tgaccaaata ttctttattc
     9961 tatccatttc acacatttct cttatatggt ctatttttaa ttttcccata ggttttcttg
    10021 cttcttccat tgttacatct atacctcttc ttttaaatat ctctataaat acattaagag
    10081 gtgcaaaaca tccataatct actgttgttc cagcccaatc gaatactact gcttttattt
    10141 tttctccata tatctttttc atatttatag cctccataga ttctttttag aatttttgtg
    10201 tttttaattg tttttacttt taaagtataa ctttcatatg ttaattatac atcatattta
    10261 ggttaaatat atattaaatt tctgttttta gttgtattta gttgttttta ttttgtatca
    10321 cattcctata aaatcctaag ttacaatcta aaatttactt tctaatctta atctatgaac
    10381 tgactatatt ttatgaattt agttgtattg agcatagttg tattatataa aactaagggt
    10441 ttattttgct tctatccatg ttatatttat ctcatttaac ttatccttcc attcattcgg
    10501 aggttctaca tcacaaataa caaaatctaa ttcctctaaa tctgctatct tatacatact
    10561 agcaattcct attttagaac tatcacataa tacaaaactt tcctttgaat tttttatata
    10621 ttcttttgac aacaatgcct tattttggtc tatactacta attccaaaac tatcaagtat
    10681 accttcacta gatataaaag ctttatctac gtataagttt ttcatagtat ctattgacat
    10741 tggacatgct gtcctttggt gttttaaatt tacttttcca ccaataaata taacttctcc
    10801 atcaaacaaa ccttgattct cataagaaat caattttgat agaactggat aagagtttgt
    10861 aataattttt aaattgttta cagatattat ataatctaca atctgcaaat tggtagtacc
    10921 ttcatctatg ataattgact cattgtcatt tacaaactta gctgcatatt tagcaatact
    10981 ttgcttttta tctaggttag tattgtttct ctttatatgt tctggttcaa tcactccata
    11041 atttattttt atagcaccac catatacctt ttttaattta ttttcatttt ctaattcttc
    11101 caaatatctt cttattgttt ctgatgatac ttctaatctt tttactaatt ctttagtgtt
    11161 taccttccct tttgaatcta ataatttaag tatttctttt tttctatcta tcgaatttaa
    11221 agacattcat tttcctccta aatataatat atttttctcc tataatatta caatgatacc
    11281 taagatttat gaaatcatat ttatgtatta aattttttac ttaattaaag ttattttcaa
    11341 aagactttgt attatcatta aaaaagtcca tcaaatatta gtatcagatg gactttgtaa
    11401 aattttattt tataaattaa tatttaaagt ctaactacta atttattttc ttgtctccaa
    11461 gaattttact aaattattta cagtagacat atcttttctc tctaaatcag ttggttgaag
    11521 ctctatccct aatctatttt caatttcaag taaaacttca attatagcta atgagtctaa
    11581 aagttccgtc tcaaataaat taaggtctaa atcttctctt atctcatcag tccctaatac
    11641 atcttcaaat atttctataa ctgtttcttg cataaatttt cctccttaaa attaagctaa
    11701 ataacctgag aatatcagca atccaaaaca cacaatatgg aatgtaatga aaatctgcac
    11761 tctctcgaac catttatctt ttttatgttt tttataaaat ttagattttc gctgatatat
    11821 atcagttaac acaagtgcca atccttggta tacaccataa gcaacataat accaagttag
    11881 accatgccaa aatcccattg ttatcatggt taccatctga gccacatgag cagcagtagt
    11941 ccttttctta aatcttttct ttctcattga tgacataaca aatctagaaa aaatataatc
    12001 tccaaaccat cttgaaagac ttatatgcca tcttgtccag aactctttca tatctttact
    12061 gataaatggc ttgtcaaagt ttataggtac ttgtattcca aatatgtacc ctgtacctat
    12121 agcaaataaa ctatatcccg caaaatcaaa gaacaggtat aaactatatg cgtacatata
    12181 acttacaata tttataaaac tcatatcttt aggtattctt gatacccaat atgtatttat
    12241 caaaaatgct ataacaaact tataacctac tcccatgaca atatttttta ttcctggcag
    12301 taagtattct tctatatact cttttctaga gatttgtttt tccaagtcct gttcaaatct
    12361 tcttgaccta tcaataggtc cagaacttaa agttggaaaa aataacataa aataaagcat
    12421 ctttgaaatt ttaacttctt ttatagctcc atcataaatt tcaataacca tttgtatagt
    12481 tctgaaattt aagtatgata ttccaataaa tccaattatt ccaaaagaag taactggaga
    12541 tattttattt attattattg ggagcattga tgcaaacaag aatccccaat atatgtattt
    12601 atttttagtt tttcttctta catattcgta tcctttaact attattactt caagaataat
    12661 aaatattacc aagtacttta gttggacatc aattcctact attaagaata tcataaacaa
    12721 acttgccaac ataccataat actttatatt cttgcccatt agacctaaaa ttactgctgg
    12781 tattgatgtt aataataata tataaagata aaaataatct ccatattgtg aaaaaatcat
    12841 taaatttcct ccataagttt ctttctgtct attttaccat tgatatttgt tggaaactca
    12901 gagattattg aaatatttct tggaatcata tatgaaggta tatactttcc aagttccttt
    12961 tttattatca tcccattttt aatattactt aaatcattct tttcatttaa ctcaacaata
    13021 ccttttaagt aagcaatttt ttcgtcctta tatactggta aaactactgc attttttaca
    13081 ttatgtactt ttctcaaatt attttctata tcttctattt caattctaaa tccatttaat
    13141 ttaatctgaa aatcttttct tccacaataa tatatatttc catctaaaag atatcccata
    13201 tctccagttt tataagctct ccactttaca ccatctattt catcatagaa aaatacttca
    13261 tcagtctttt ctttattatt gaagtaacct ttagaaactg atggtccaat tattataatt
    13321 tcaccctttt cattttcttt aagttcattt ccatcttcat caagtatttt tattttacaa
    13381 tttgacatag ggtatccaac tggaagactt ttttcatcat ctatagcctt ttgagtcata
    13441 tcatttacac ttacaccaac agtagcctcg gttggtccat atccattgat aattcttgtg
    13501 tttggaaatc tactcataag ttctttagta agattttttg aaagtgcttc tcctatgaat
    13561 atcattgact caaggttagg aagcatttta ctattaaact ctttttcagt tatacatact
    13621 cctgcaaaag aaggagttga cacccataca gctatatctg agatactaaa ttgtctaaat
    13681 agttctttat agtctgctaa tacatctttt gatattgaaa ataatgtagc tccatggata
    13741 agaccagggt atattgttgt aactgaaagg tcaaaagaat atgctggttg gttcattatt
    13801 actttttcac ttccatctat gtttagatat gggcttatcc aatctgaaaa gctatctaaa
    13861 ttgtttgaac ttatttgaac cccttttggc tttcctgtac ttcctgaagt aaatagtata
    13921 tatgcatttt catcatcttt tacccagttt tctttatcta atgattttcc ctgatattca
    13981 tttattatat aatttaattt atccatatca attacattga tatctccaaa atttctttca
    14041 tcactaaagt taaataatac ctttggtttt atctctttag tgacttcaaa aactctatca
    14101 attggaaaac taatatcaag aggcacatac gctctacctg attttaaagc tcctatcata
    14161 catgccatta tcatgttttc tttatttcca taaattacta taggagtatc ttcttctttg
    14221 tatacatcct ttagaaatac tgatatagca tctgaatatt catttaaatc tttataagaa
    14281 agtttatctc cattacacat tagtgctgtt ctatctgtat ttgaatattt ttttattcct
    14341 tcaataattt tcatataaga atgctccttt aaaatccgtt ataaataaat ggcaattctt
    14401 taaatggagt aaatgttaga actcctatcg ccaatattat aaccatagtt aaacaaatac
    14461 tagttaacaa atctttattg ttctttaaat attttaaata atctctcaca gacatctacc
    14521 catcctttcg taccaagatg cattacatcc cttaaataat acttatctga ccctttatct
    14581 ttaaggttta gaacttcaaa gcctttgctt tctgctattt tttgtgcttt atcgtagtac
    14641 tgatttcttt ctttttcaga aataccagtc aaattataga atttatccat acttgggatt
    14701 aatacaacaa ctggcttaat acctaagtca gtacatacat ttagcatcag tttataattt
    14761 tcaaactcct tagatgttaa taaatttaca tctttgtctc ttcctttgta ttgctctata
    14821 ccatctttaa aatgctcttt ataatagtac ttatcaatat ttagtggatt tttaccaact
    14881 ctttttttag catcttcaat ggctttcttt ctctcatatg accaatttat tggtcttttt
    14941 ctttttgttg ctctcttttt atctagctta attaatcgtt tataaagaat acccttttct
    15001 ttaagagcta tacagtactt tcttccttga aaataaggct ctaaaagcac cttttcagct
    15061 ttttcaggaa atgtcttagg ctcatatagc ttagcatata atgcttctgc tttatactcg
    15121 tcacttcccc ataataactt acttgatttt tttgcatatt caatcttatt ttgtttagat
    15181 atttttggat tatctaaaaa tcgataaaat tgtattggtg aaaatctaga ctgataatgg
    15241 tgacttgtga ctccgtcttt ttccataaac cattgcatag aaattaacaa gacaactttc
    15301 ttattatcaa tattaggatt catacttcct aatattgcag catcttgtaa tgtctgagta
    15361 taggctctcc caattgcaaa caccttattt ttactcctat ttgtattaaa atagtacttt
    15421 ggatgttgtt ttgttgaatt actaagttct gatgacccca aaatcataat atccttttct
    15481 cttaaaaaat gattatttgc tgtaacccct ttatctttta tatcacttaa agtatcatcc
    15541 ataattggaa gtagattttt ctccctcaaa agttcatcag ttttagaatc aagaaatttg
    15601 tctaacccaa ataaaaatac tacgcctatt ataaaaggtg taataaagta tatcaatttt
    15661 ctcatatttt ctctcctcta aaaatattca aatttatgtt attattaatc atattagttt
    15721 tgatatgtat attcaactac aaaattgtaa taaaatcgtc aaaacttttt ttgatactgt
    15781 tacaaaattg taaaaaaaaa gaaaaaaacg ccaaaaataa atttcttttt agcacttttt
    15841 catacttata atttgacatt ttaacacttg gctaagtata caccttacct taacttaaaa
    15901 ttaaatttat ttaagaatat atattataaa ttcttatatt ttctaatata aactatatac
    15961 tatcagctgg ataaacaata ctactttgtc ttcttatctc atccataatt tccattacta
    16021 ttattgaatt ttcatgagaa tttatatttg attctatttt attgttattt ataagactta
    16081 taaactctac aagttcataa tacattcctt ctatttctct aggcttttcc tctgcttttt
    16141 cttttggaat agatatatct tcttctcttc catctctgta tttaataata gctttttcaa
    16201 atagattaag cttttcaatt attatacttc cctcttctcc ttgtatttca gatggtatat
    16261 aagaatcagc tactttagaa tactgaatta tcgcatccat atcatcatat tgaaatacag
    16321 ctgaaccttc tccatccact ccagtttgaa gtatatgaga aactgccttt acagtttttg
    16381 gagcgccaaa taaattcacc ataggatgta tacaatacac tcctatatcc attaaagctc
    16441 catttgataa ctctttttta aaggcatttt ctataattcc atttttatat ttatcatatc
    16501 ttgaagaata ctgacaatag cttccaaaaa atcttcttat tttccctatt ttatataagt
    16561 tttctttaac tgctttaaag tttggaacac atgtaattct catagcttcc ataagcaata
    16621 cattattatc tttggctgtt tttatcattg atttgacttc ttttaaatta gatgctaacg
    16681 acttttcaca aagcacatgt tttttgtgtt ttaaacaagt aatggtttgc tttgaatgta
    16741 atgcatttgg agaagctatg tatacagcat ctatatcctt cgatttagcc atttgttcta
    16801 aatcatcaaa aaaaatactt gctccatata gagttccaaa ttctctagct ttttctaaat
    16861 ttctagaata tacagcaacc aattcaaagt ctttaaccct agatgcagct cttagaaata
    16921 tatttgctat attgcttgtc ccaacaatac caaatcttat tttacccatc ataaatcact
    16981 ccccattttc tacactttat tttaatttta ataaaaaact ttacaactta tagatatatt
    17041 ataaaataat tttatcaaat aaaaaagaaa gctaatttta aatgatttca ccattttaac
    17101 atagctttct tttaactaca atctaaattc aacttatatt tttaaagtta tctttctact
    17161 atatctttat ttcccctcaa aaattttata gtaagaattt catccacaaa caatgttact
    17221 aaaagtatag ttacaacaat tggccaataa ggtattataa gactatataa actatcaact
    17281 atatacactt gaaaatatct aactccaata aatgtcaata tagtccaata tacagaaaac
    17341 tttagacata ctattcctct actattgtac ttataatctg aataatccca atagacctta
    17401 ttaaaatata ctttcataag tattcctgta atatattcta ctactgtcgg cactactaga
    17461 cataataaaa gcaatgcaaa tatattattg gggtcaataa atcttgagac ctctattata
    17521 atcgacattg caattgcata cattggttta aatggaccat ttaaaaatcc atcttcttga
    17581 aaatgccctt gtttataata actatataag ttttcaatta tccaacctat aaaaccatac
    17641 aagaaaaaat taaataaaaa atataataga gaacctacat tatccatgaa tattcctcct
    17701 agctgtttat acttattttt tctcatattc atggatttat tctaaatcaa agaaattata
    17761 tttaatttat ttaattaata ctttatatta tagattgtga cattctttag agttcaaact
    17821 ttttaggtat tctctaactt ccagtaaatt ttcaagtatc ttatgtgatt gtgatttaat
    17881 ttgctcatct ggctctttta aatctggtac atttatacat cttataccac cattgtatgc
    17941 agcttcaact cccataggcg aatcctctat gacaatacaa tttttgggat taacatttat
    18001 ctttttagct gcttttagga atatctcagg gtttggtttc gagtttacaa catcatctcc
    18061 acaaactata gcatcaaagt aatcttttaa atttgcctta gctaatctct ttactgctct
    18121 ttctctcttt gttgatgttg caacagccat tttgtatcca ttttccttta aaaaagagat
    18181 tagttcattg actcctaatt tgataggagc tcccttcctc tccataaatt caatcatatt
    18241 tttagttttt tcgtcatata aatctattat tggtacagaa ctatcgtata tatctgtaag
    18301 accttctatt attcctttac gatttcttcc cataactgat gtataaattt ccttagtcat
    18361 agtatatcca tacttttcaa atgtctccat ccaaaattca agagaaattc tctctgagtc
    18421 aaacaaaact ccatccatgt caaaaataat tccttctact ttttgcatac aattcctcca
    18481 atattttttg atataattat attacacttt ttatgattaa ttttacaacc ctcaatttat
    18541 ccaattctaa tattgtaaaa ttgtaatttt caaaagttac actttggttt actttaatat
    18601 ttgaaccaag catataggat atccatccac ctatactgtc aaatccatca tgctctatat
    18661 caagtccaaa atacttatta atatagttga tagattcagt tccatcaact aagtaagaac
    18721 ctgctcctat tttcttaata tgagtctctt ctttatcaaa ttcatcttga atttctccaa
    18781 ctatctcttc tagtatatct tctattgtta ccacaccaga tgtaccacca tattcatcca
    18841 caactatagc aagttgaagt ttttcctttc ttattctctc aagagcttta tctatagtta
    18901 aattttcaga tatatatata atatctctca atatttcttc tagttcaatt ttattttcat
    18961 ttatcttttg attgtataaa tctcttatat gtacaaaacc taatatatca tctttatttt
    19021 ttctgcatac tggatatctt gtaacacctt cctcttttaa aatagctaat atcttttctt
    19081 cagaatcact ttcatatata cataccatat cagttctagg aaccattatc tctctaattt
    19141 tcttttcttc aaattcaaaa atattatcaa ctaatctttg ctctgattca tctataagtc
    19201 cacttttata actttcttct actaatagtc ttatttcgtc acctgtatgg ggttcatcaa
    19261 cctcatcagc ttgtgaatat ccaaatggtt taagaaataa atcagtactt aagttgaaaa
    19321 aatatattat tggatatgtt aacttataaa atcctactaa taaaaaagat gtactcaaca
    19381 ttattttttc tgtattataa agtgctaaag ctttaggaac caactctcct attacaacct
    19441 ccatcattgt tataattaaa aaagaaatgg aaattgatat agtatatata atactttcac
    19501 taagattaaa tacacttact attggtaaaa ttaactcttt aactgtagat tctcccatcc
    19561 aacctaatgc aagtgaacaa agtgttatac caagttgaca tgcagaaaga taggaattta
    19621 agttgtcttt tattatttta catctttttg cattattatt accttcagtt atcatggtat
    19681 caatcctaga actacgaagt tttaccatag caaattcaca agctacaaaa aaagcattta
    19741 tcaaaacaag cattactata agtgttactt ttaataactc ccgcataaag tattggttct
    19801 tataatgtaa gaaatctata agaactcaaa gttcccccct tatatttata aatttaattt
    19861 ataatatata ttctatcata tttattaata tatcctactt tattacaaat aatttttata
    19921 aactaaataa ccccttttaa ta
//
LOCUS       DACXMZ010000055        19856 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.055, whole genome shotgun sequence.
ACCESSION   DACXMZ010000055 DACXMZ010000000
VERSION     DACXMZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.055"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            6..473
                     /locus_tag="JD016_07675"
     CDS             6..473
                     /locus_tag="JD016_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAU4999517.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGCCLLINTIIECVIG
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            484..699
                     /locus_tag="JD016_07680"
     CDS             484..699
                     /locus_tag="JD016_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999518.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            1140..1313
                     /locus_tag="JD016_07685"
     CDS             1140..1313
                     /locus_tag="JD016_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HAU4999519.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
     gene            complement(1469..1762)
                     /locus_tag="JD016_07690"
     CDS             complement(1469..1762)
                     /locus_tag="JD016_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999520.1"
                     /translation="MDEMLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICI
                     IEKEHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     repeat_region   1922..3128
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1988..2016
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(3959..4831)
                     /locus_tag="JD016_07695"
     CDS             complement(3959..4831)
                     /locus_tag="JD016_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAU4999521.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(5225..5803)
                     /locus_tag="JD016_07700"
     CDS             complement(5225..5803)
                     /locus_tag="JD016_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAU4999522.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(5861..6577)
                     /locus_tag="JD016_07705"
     CDS             complement(5861..6577)
                     /locus_tag="JD016_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAU4999523.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            6768..7853
                     /locus_tag="JD016_07710"
     CDS             6768..7853
                     /locus_tag="JD016_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAU4999524.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(8008..8667)
                     /locus_tag="JD016_07715"
     CDS             complement(8008..8667)
                     /locus_tag="JD016_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAU4999525.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(8884..10176)
                     /gene="lysA"
                     /locus_tag="JD016_07720"
     CDS             complement(8884..10176)
                     /gene="lysA"
                     /locus_tag="JD016_07720"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAU4999526.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(10194..11387)
                     /locus_tag="JD016_07725"
     CDS             complement(10194..11387)
                     /locus_tag="JD016_07725"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAU4999527.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(11338..11577)
                     /locus_tag="JD016_07730"
     CDS             complement(11338..11577)
                     /locus_tag="JD016_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999528.1"
                     /translation="MMKGYIAEEILLTDKFSGLMQNMHKAVTFKVVSYKVTWSMKFTR
                     PKYTFGRFYLSKFSLRGRKHEYNCSKIWWKFSSRY"
     gene            complement(11748..12395)
                     /locus_tag="JD016_07735"
     CDS             complement(11748..12395)
                     /locus_tag="JD016_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999529.1"
                     /translation="MQIILQGECTMTNMEKEILLLISRVRCVTESQFRKIYGDQKRYN
                     KKNFKKTLRKMCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSEL
                     VVRINTAGCDVKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYEN
                     LSKKIDKSTIPFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(12535..14235)
                     /locus_tag="JD016_07740"
     CDS             complement(12535..14235)
                     /locus_tag="JD016_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999530.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(14632..15198)
                     /locus_tag="JD016_07745"
     CDS             complement(14632..15198)
                     /locus_tag="JD016_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999531.1"
                     /translation="MMNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYS
                     FGRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYR
                     EDYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNL
                     SGLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(15356..16069)
                     /locus_tag="JD016_07750"
     CDS             complement(15356..16069)
                     /locus_tag="JD016_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HAU4999532.1"
                     /translation="MGKVAKLLLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            16361..18025
                     /locus_tag="JD016_07755"
     CDS             16361..18025
                     /locus_tag="JD016_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HAU4999533.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(18132..19031)
                     /locus_tag="JD016_07760"
     CDS             complement(18132..19031)
                     /locus_tag="JD016_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAU4999534.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(19058..19696)
                     /locus_tag="JD016_07765"
     CDS             complement(19058..19696)
                     /locus_tag="JD016_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HAU4999535.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
BASE COUNT         6603 a         3193 c         2126 g         7934 t
ORIGIN      
        1 attttatgat tacaaaattt tttagtaata tattatcagt atttctatat tttctattat
       61 cacttttagg agttgctggt ataatattct tatgtatgat acctatgttt ttatatgatt
      121 ttagttctga caaagtaatc gctttaattg caagaatact atatttttct gactacttta
      181 tagtcatctt attcttgata tttataatta atagtactaa gtattcccca ttcgtacttg
      241 aaaatgtaaa aaggtttaag gtaatgggat gttgtttatt gataaacact ataattgaat
      301 gtgtaattgg ttataactca aatactagtc ttataatgat atttggagat aatagtggtg
      361 gtatctcacc acttatggtt atttctttta tatttgcact aatgtgcttt gtaattgcag
      421 aaacatttga taaagctata aaaataaaag aagataatga tttaacgata tagaggtgat
      481 gttatggcaa tagtagttaa cctagatgta atgatggcaa aaaggaaaat atcacttaaa
      541 gatttagcag aaaaaataga tattacaaat gcaaatttat caattttaaa aactggaaaa
      601 gcaaaagcta ttagatttac cactctaaac gaaatttgca aagctttaga ctgtcagcca
      661 ggtgatatac ttgaatatgt agaagatgaa aatgattaaa aaagagtgtt tagaaacact
      721 cttttttatt atactatatg atttttattc ttttactata ttattgacat taatattaac
      781 tttgtttaaa gtcataacaa aatatgttct aagaaagaaa ctgttccttt atctatttgt
      841 tatccattac ctataagaac tgtatcacac taaataatac ttttaaattg ataattttac
      901 cacagattcc tatatacatt cttttctacc taatatttat ttaaactgtt agcaaaacat
      961 ggaacttttt catttactta tacccccatt tatattattg catagtttat agctacgtgt
     1021 tataaattac attattgatg attttatcta acatttgtaa tcttttagat actctttgaa
     1081 gttacaacac tgttatactc taattaagta aactaaatct gatacaagtt ggtgattata
     1141 tgaaaagaaa actttcaacc tattttaaag gatatatttt aggtttatgt atagcatttt
     1201 tattatttac ttttatatta aaaaaattaa ttatagaaaa tatcattgga ataataattg
     1261 gaactggaat tgcattttta ctgatttacc tatttaataa aaaaagaaaa taatattcta
     1321 taaaaaggtc ttttagtgaa tttacaaaaa attaataaga ataatatata cttagtattt
     1381 aaaaagggta acaactctct ctagctgtta cctcatatat ttatctatat ctttagtatc
     1441 aagtttttta atttctactt tttaattatc aatttctgta tgtagatata ctaaaccttc
     1501 caacttgaga tttaaataac ttttcaatct cataaactat tctatacacc ttattttcgt
     1561 actctaaatc ataatacaca tttatttcac ctatactgtt tacacttatc gtataatgtt
     1621 ctttttctat tatacatatg cttccaaaat ctgacaggtc attatctatc aattttaact
     1681 gtttttttat ttttgaaaaa tctaatcttg gaaatatgtc attaggataa aaacttttat
     1741 tatatacaag catttcatcc accccttatt tattctattt atatcatttt ataccctaat
     1801 taataattta ttaatcatct ttttccatat tattcaaatt ctcttatata aaaacaaaat
     1861 caatttagaa tatgtattat taaatataaa gtgactcaat ctttatttaa taagaaaaaa
     1921 catttatatt acatatagtt aatataaaac taatagttaa tgaagcattg caataggggg
     1981 gtattgtatt tacattccat atagttaata taaaacccaa tgtaataaat gaccatgtgc
     2041 gagaagagga aaaatttaca ttccatatag ttaatataaa acttttcatt ggcaccaata
     2101 cttctaaatg ttccaatatt tacattccat atagttaata taaaaccttt gaaaaagaga
     2161 tagataaact aaatgtatct tatatttaca ttccatatag ttaatataaa actatctaat
     2221 cgacgaacag gtttatcgcc attttctata tttacattcc atatagttaa tataaaacga
     2281 tttgtaacat taaaaaagaa taaaactttc tcagatttac attccatata gttaatataa
     2341 aacgatgtaa taaatgagta ttcagtgcat ttcaaaaatt atttacattc catatagtta
     2401 atataaaacc acatattgtt aacagctcta tagcaatgat tgaaaattta cattccatat
     2461 agttaatata aaacattatg agtatcggga aagatgttgt gagaggctta atttacattc
     2521 catatagtta atataaaact gatgaaatac atcaaactta tacagatgca tatagattta
     2581 cattccatat agttaatata aaacattgta tgatttattt ttatagcggt actcctggga
     2641 gatttacatt ccatatagtt aatataaaac ttattccaag aaggacgagt tttagcattc
     2701 caagaacgat ttacattcca tatagttaat ataaaaccat tatgctatag tttcctagct
     2761 ctccgacacc tacatttaca ttccatatag ttaatataaa actacaccag gttataatga
     2821 aaatggagca ttagggaaat ttacattcca tatagttaat ataaaacgag tctaaattac
     2881 aagcagaagc ggaaaaaatg ataaatttac attccatata gttaatataa aacattgtat
     2941 gttgatatta ctattctttc agttaaaaga tttacattcc atatagttaa tataaaacgt
     3001 ggttggaact tataaaaaag atgctgataa gttatttaca ttccatatag ttaatataaa
     3061 acgttctgtt gcaacttttg aaatgtattg accaagtata tttacattcc atatagttaa
     3121 tataaaaccc caaaataaat taagtatttc caatacttat acatatacaa ttctcctaaa
     3181 cttgcagtga gcgagcaata gtgcaattga taacagttat catacactct caacgcttta
     3241 tatttcaact attaaaccct actttatcac aaaaatcgct cactgcaaaa cttttatatt
     3301 tttattatat cataaaactc atactataaa ataaaaatgg gataatttta caaactaaaa
     3361 gcatctacat ttttataagt atttattata ttctctattc caatgattcc tttttctttt
     3421 tattgcatcc ttcctatgta atcgcacctt agctcaataa gtcttttatt ttcattaaca
     3481 attttttaaa ttattcaaaa tcatcaaatt tttcatctta tacagttaaa tcaaaatatg
     3541 tgttatatct ctttatctat atacttttct tccttccatt tcttttcctc tttatacatt
     3601 ttcactttgt tactttacaa tctcaaggtt attatctgct attaaagttt tatctttcca
     3661 ggtttcacaa aaataaattg aataagtgca aaaataacag atacaaaacc ataactattt
     3721 tcttttctat aaaaaaatat ataatcaaca agaaatataa taaatgaaaa taactatgaa
     3781 taaatactct catttagaag aaatagacaa agatattcct taaaatttaa gcaaaatatt
     3841 gtttaaataa aaaaactgac tgtcagttaa atgccagtca gctattttat atattttatc
     3901 caaacatcac tatatttgaa cattttaata attaataata cttattaata tacaaacttt
     3961 actcatacac agaatatctt ttccaaattt gctctaaatc atcaaaatgc tgtttaatat
     4021 cttctttttc cttcatccca acagatatag caagagcatt tgaaatacta agagctggaa
     4081 ctagagaatc tacaaatgaa gccatattac tcttaactag aagagtgtta tctgcaagtg
     4141 atgctactgg cgcaaataag ctatctgtta atgatattac atgggctcct ttttctttag
     4201 cataattgac aatttgataa gatttcttag aataacgagg gaaactaata gcaactatta
     4261 catcttcttc atttattctt acaatctgct caaaagcatc tcccatatcc attctaatta
     4321 tatgaacatt gtcaagtatt atatctaagt aaaaacctaa atattgagct actacaaaag
     4381 aactcctcat tccaagtata tatatttttc tcgctcttaa aagtttgtta gaagcttctt
     4441 taaacgctct ctcgtcaatt tcttctagtg tagaccttat attatctata tcacttttta
     4501 aaactttgtt tagtattgca aaatcatcgg aataatcatg tgccatttca actctttgta
     4561 ctgtagttag tttatttttt atgagttctt gaagtgcagc ttgtaattta ggatatccac
     4621 tataacctaa tgcatttgca aatcttacaa ctgtagactc acttactcct acagtttcac
     4681 ccaatttaca tgcagtcata aatgcaactt tatcataatt atttaaaatg tattgtgcta
     4741 taagtttttg acccttactc aatctcgtat attgagattg tatatttgat atcaaatgtt
     4801 tactgtcctt catttcattt gtttcttcca tttttttctc cttttagtta tataaactat
     4861 tttgttttat taacactgtc tttttctcta aaaattttat tttttatctc aatttcatct
     4921 tagtttttat catagcttct taaactcata aaattctaac ttttctgtac cttatacttc
     4981 acatagtatt ttcgattaaa tttattatac catctttcct tgcatttttt aatatttatc
     5041 caatatttct tttaaatttt tcaattttta ctcatttaaa agttatctat cttattttgc
     5101 atatttagat gtatattttg ttttgaaaat aagctgttgt atacttatta tttaaagtat
     5161 ttttaatagg tcacactata tatattaact cttaatacaa tcttgtcaag ttttttagta
     5221 tttattatgc ttttttcttt tcaattaaaa tcaattgtgg tgggttattg acttgattta
     5281 aaaacttaga ttctaaaaca ttaaattcat cttgatttaa attattgaca aggttatata
     5341 tgcaatcttt ttcttcttga cctccatcat gccctgtata tatggcaatg cttattactc
     5401 cattttcttt taacatttct aaacttaact ttatagcctt taaagttgta tctggtttgg
     5461 ttataattga atgtttcgct cttggaagat aacccaaatt aaataaaaca catgaaactt
     5521 cttcttttat atattgattc attttttcat gcccatcaaa tatcaatttt actctatctg
     5581 tatatccttc tttctctagt ttctttcttg tagactttat tgcttcttct tgtatatcaa
     5641 aagcatatac aaagcccttt tctccaacct tctcagctag ataccttgta tcatacccat
     5701 ttcccatagt agcatcaatt actatgtcac cttcatttat aatattttct aaataaaatt
     5761 tatttatatc tgtaatttta gttaaatact ttgcatatgc cataatacta tttctcccta
     5821 ctattaactt tatttcattt tttctcaatt tatttttatc ttattttatt gattttaaat
     5881 attttatttc atcttctgtt aggtatctat attctccaac tttggtgtct tttaatacta
     5941 ttttccccac agaaatcctt cttaaattta aaactggatg atttatagct ttgcacattt
     6001 ttcttacttg tctatttcta ccctcatgta tagttatttc acacactgaa taattttttt
     6061 ctttattttc tttagttacc cttattttgg caggtgccgt tgtgtaatct tctattttta
     6121 gtcctgtttc gaattgtctt atctcatcat cacttataat tcccttcaca cttgccacat
     6181 acgttttatc aatttcatgt tttggatggg taagcttata tgttaaatcg ccatcattag
     6241 taagtattaa taatccacta gtttcataat ctaatctccc tattggatat actctttccc
     6301 ttacatcttt aagtatgtct attacactgg gtctattgaa ttggtctttt actgtagtta
     6361 tatatccctc tggtttattt aatactatat atatataatt ttcacttggt tttacttttt
     6421 tattatcaaa ttctactatg tctttttctt catcaatatt aaaagataac tcttttacta
     6481 cacttccatt tacctttatt cttcctccaa gaattatttc ttctgctttt cttcttgatg
     6541 ctattccaca agaagctata tacttattaa ttctcatact taatccttcc ttatcttaaa
     6601 ttatgtgtgt ttaatatttt tatcaatgtt aattttgcta cattattata ctatactaga
     6661 ccttaattaa aaacaagcaa aaaaattatt taattttcct aaataattgt aatttctttt
     6721 cacatactat taatatgtaa tagctgacta aacactattt taaggaagtg attattattt
     6781 caaaggaaaa tataaaatat tatttaatag tcgtgtttat tggaatttta ttttttaaat
     6841 ttattaatac tccatcagac tttatatcta gtattgaagg attcttaaag ttttttagcc
     6901 catttttatt agctatttta cttgctttac ttttaaatcc acttgtgatg ctttttgagg
     6961 taaaattcaa agcacataga cttttagcca tttttttatc ttatatattt ataggtttta
     7021 ttttagcctt tgccattagg ttattaattc catcaatagc taatacatta aatagattaa
     7081 taaatgaaat gcctatgtat actgattata tagatagttt tatagaaaaa aatatgtcaa
     7141 acatagattt tctaaaaact ttaattcctc atatacaaca tagtttggat aatttattaa
     7201 aagaagcaag taattttgtt ggtaaaattc ctaaaaactt cttaatctat acacttagta
     7261 tatcttctat gctatttaat atgacaatgg gatttatact atccatatac attatatatg
     7321 acaaagaaaa aattgcattg ggatttaaga gatttctata ttcttcaact gctagaaaca
     7381 aggcagataa cattatagaa ttttttagga ctactcatga tattttctat gattatttac
     7441 ttggtagaat attagactct cttattattg gtataattgc atttttagga tttcaatttg
     7501 ttatcagaat agaaaatgct ttatttttag cttcaataat attcttaggt aatataattc
     7561 cctattttgg tccttttatt ggtgcaatcc ctccaatagc catgactata ttatatagtc
     7621 cacaaaaaac tatatgggtc attgcatttc tatttatatt gcaacaatta gatggaaatt
     7681 ttatagaacc aaaagttatg ggtaatcaag ttggtatagg agctatatgg gtaatatctg
     7741 ctattttggt gggacaatct ctatttggtt tcataggagt ttttctttca gtaccaatag
     7801 ctgctgttgt aaaaacttat gttgataaat atatagataa tcgtttacaa tagattatct
     7861 ctactgtgac tttaattttt agtttaaaat ttaactgcta tttattaaat tatttctcaa
     7921 aaaatatctc ttagtaagaa aaaattgcta agagatattt tttataatat ataatttgtt
     7981 cattaaattt atgcatatat tttatactta ttgattattt gagttttgag aatttccacc
     8041 actatctgaa ccaccagtag tatcttgtgg agttggtgct ggtgttggat tactattatt
     8101 tggcttttct tgattttgag aagaactgtt attattgtca gacttattac tatctgattt
     8161 tttatcattg tttttagaac tagatttatt atttttacta cttccactat aagaagattt
     8221 ccttgcatct gattcaaccc tatacctatc ttctccagcc gtatcttttg cctttttctt
     8281 tctttgttcc tctgcttcgt tttgttcttt ttctaattta gttaactctt ctttcttttt
     8341 agttgcctct tctttaataa cagaggaacc attcttgatt ttctctataa gtttaagttc
     8401 cttgattgca ccctcataat tttttaattc ttcaaactcc gtcatatttg acattctcat
     8461 agcttgcata tacatagctc ttgcattctc attagtacta tcttcttcca gtgcagagga
     8521 taatgattcc atagcttttt tatagttatg tttttctaaa tatgtttttc cttgctctac
     8581 taaatttatt tcatcttttt tattaaatga acatcctaca agaaatatag ataatatcga
     8641 tattaataaa attactcttc ctctcattaa atccctcctt gtcatatact ttaaatttta
     8701 tcataattat aaagttttta aaatatttta tatttattac aagaaatttg ttagtaaaac
     8761 aaaaaataag cagatatgct acgaacgata caataaacca aaaattaatt tagagataca
     8821 tatcctagca aatctgctca taataaactt taatatttaa tttttattat atacttctct
     8881 attttatatc tctaaagcta ataagtcctc atagctttgt ctcttacaaa ttatcttatc
     8941 atttccatca aatactgata ctactgctgc tcttggtatt ttattataat ttgaagacat
     9001 tgaatatcca tatgcaccag ttgatgttgt tataagtata tctccacttt taatatccat
     9061 tatgtttata tctccaatta atatatctcc actctcacaa cattttccag ctattgttac
     9121 atgattcata gcctcatgat taattttatt cacaatacta cactcataac tagcttggta
     9181 caacgaaggt cttacattat ctgtcatacc tccatctaca cttacataaa ccctaacatc
     9241 ttttatatct tttatagaac ctactgtata caatgtactt cctgcatttg caactattga
     9301 tcttcctggt tctattacca atgtaggtac ttctattcca agctctttac atgaagtttc
     9361 tgctttagtg attatggttt cacaaaactc ttttatagtt ttaggtttat ctccttcgct
     9421 ataataaacg ccaactcctc cacctaggtc aatttctgtt agttgtatgt tgaatttttc
     9481 ttttatttct tttaccaagt tcatcattat atccactgta tctatgtatg gttctacatc
     9541 aaatatttgt gaacctatat gagcgtgaag acctacaagt ttaatatttt tatattcact
     9601 taatttttct attgctctaa ataaatctcc atttactaaa gcaaatccaa atttagaatc
     9661 tatctgtcca gtttttatat aatcatgtgt atgagcttct ataccaggag ttattctaaa
     9721 gtatatctct tgagttttac ctttatcttc acataatgct tcaagcaaat ctaattcata
     9781 aaaattatcc actacaaatt tacctacacc taaatctaat cccatgttta tttcttcttt
     9841 agtcttattg tttccatgaa agaaaacttt ttccatagga aaatttgatt tataagctgt
     9901 atataactcg cctcctgaaa ctacatctaa acacatttgt tcttcattta taagattaca
     9961 catgtaaaga ggtaaaaaag ctttccctgc ataagcaacc ttattttttc cctctctagc
    10021 cttaaaatat tctatatatt ctctacaatt ttgtctaact aaagcttcat caaatacata
    10081 aagtggagtt tgatattttt tactaagttc taaacaactc actcctccaa tatataattc
    10141 atcattattt acagtcatag ttccatgaag tttcataata gcctccccaa attttatata
    10201 ttaaatactt ctgctatttt ttctatagct acttgcttat catcagaatt tatagcacaa
    10261 gttatacgaa tctctgatgt agttataagt tttatcatta taccattttc attgaatatc
    10321 ttaaatactt tagcagcaac tccagaagta tttttcatac caagacctac taaagaaaat
    10381 ttagtgatat tattatcaat cactacatgt tcttcatctg tatatttaga tactatcttt
    10441 ttacattctc ccaactcttc ttttggaact gtaaatgata cacttatctt atctaaaata
    10501 ggtgcagttt ggcttatcat atctacactt atacctatag ttgctatatc ttcaaataag
    10561 ctagatatat tttcagcttt aaaatctttt attgttatag aactgtcatc atcactagta
    10621 gctaatcctg ttattacttt atcctctaat ttcatgtctt ttccccctct tatatatgtt
    10681 cctttttctg taccacatgt acgtccgaca tagatttcaa caccatattt ttgtgcaagc
    10741 tctatacttc ttgaatgcat tacttgtgct cctaaactcg ctaactcaag catttcttca
    10801 tattcaatct catcaagttt actagccttt gaatattttc ttgggtctgt aaaataaatt
    10861 ccatcaacat ctgtatatat ctcacatttt ccatttaatt taactgcaag agctacagca
    10921 gatgtatctg aaccacctct tccaagagtg gttacatcac catcttcgtt gattccctga
    10981 aatccagtta ctatcacgac atttccttca tctaaactat ttttaattct gtttacgttt
    11041 atatcatcaa tttgagactt tccatgaaga ccactcgttt ttatatttaa ctgataagca
    11101 ttataactta tagctttaca tccaagtgca tttaatgcta ttgataataa agatgctgaa
    11161 atcatttcac cagtagacat taaagcatct aattccctct tactaggttc attactcaat
    11221 tcctttgcta aagttatata ttcatcagtg gattttccca tggcagacac aactattacc
    11281 atttgtggat tttcttttct tcgttctatt atattttctg ctattgattt tattttttca
    11341 gtatctgcta ctgaacttcc accatatttt tgaacaatta tactcatgct ttctccccct
    11401 caagctaaat tttgataaat aaaaacgccc aaaggtatac tttggacgtg taaatttcat
    11461 agaccaagta accttgtagc tcaccacttt aaaagtgaca gccttatgca tattctgcat
    11521 aagtccagaa aatttgtccg tcaacaaaat ttcttcggct atataccctt tcatcaatta
    11581 tcaacgctta ccagctccta ttgaatacta ttgaatacag ctacctctac cctaggctca
    11641 ttatttttaa ttaatttatt aatatagttt tatcatattc tatataatat tgcaacaatt
    11701 attttacaaa atgtggcaaa tcgatagttt tcttttctaa agttagctta tatacaatta
    11761 aatagtttat ttaatcctaa atctataaaa tttatgctat actcattatc atatctctct
    11821 aaatcattta attcggatag attatctggt gtaactacca aaacgtttgg tactttaaaa
    11881 aatggaattg tagatttatc tattttttta gaaagatttt cgtatttaga aatgtctatt
    11941 tcattattta agtcaacatc aactaaaact tgctttatat taccaaattg gtctgtatat
    12001 tcaataaata aatcgtattt cacatcatct acactaacat ttcggtaaaa tctttttaca
    12061 tcacaacctg cagtattgat tctaacaact aattctgatc ctactagagc ctttattaaa
    12121 tcatctccct taaaaagttt actaccgttt aaatagtata cataagagag gtctttataa
    12181 ttataataat ccacatcaca aggatatttt tttagtgtat attcactaca catttttctc
    12241 aaagtttttt taaaattttt tttattgtat cttttttggt ctccataaat ttttctaaat
    12301 tgagactctg taacacatct aaccctgcta attaaaagta atatctcttt ttccatattc
    12361 gtcatagtac attctccttg aagtattatt tgcaaagttg tactaatatt atattaattt
    12421 tatgacatta aatttacaaa gaattaaaat aaaatagcta atttttatag aattaaattc
    12481 tataaaaatt agctatcact atgttaaata ctattatcaa tctttttatt taaattatac
    12541 attaccttga ttttcagaat ctttggaatc tgtattttca tcagttcctg ttttttcttt
    12601 tgctttttta tcttcaaagt ttgcaatatt taggttatca aattctgaca tttctattac
    12661 accagattct tcttttgaat acgatatttt gactttatca ccaacctgag tcaatggcaa
    12721 ttcgctagat acttttgaag tagccctaaa tattatatct ttatttgaat ctaaagttac
    12781 ataataatta gtgttaccac ctcgcacatc tgggcttatt cttgtcactg tgccttctaa
    12841 tacctcatct tttaaatcat tatcaagttt taaatcatta cccttagatt ctaatgcatc
    12901 cttatagttt cttaatgctt cattcttatc ttcacctatt ccaagtacat tataatcttc
    12961 aacagataca aatgcaactc tcttaacaag accagcctta tcttttaatg atgatacata
    13021 tgttggctga cccaatatat tatacattac agggaacgta gcttcataat ttttttcttg
    13081 aacattacct tcagctgatt tcattgcagc cgtttcagta gctcctggtt gtttatataa
    13141 tctagcttct tttgttctag aatcaactaa cataaatcca attgtagatt catctccacc
    13201 tgaagatgtt atacctgtat accaataaga cctgttatca ttgccataaa ctagtgatgt
    13261 accttctgtt ggtactaaaa ctcccttttc tgatataaca gagtttaaaa atccattaac
    13321 ataaacaccc cagtctttaa tttggtctgt tacaaagctt tgtggttgta ttctatccac
    13381 ccatttaggc gtgtttttaa catcataaac atttattttt ccagtttctg catcaacagt
    13441 ggctattcct gttgcatttg ctcctccata gccaatttta tgttcatata aacttacaac
    13501 ccaataaggt cttccttcat catttatttc aagagtgaaa tctgtcatac caacatttac
    13561 aataccatgt atgtataaat gtctttgtaa atcctgatgt aagtaggctt ctggttggta
    13621 aactatcttt attggctttc catctatttc ttgaactaat tggacgtctt gaggattact
    13681 agctgatact tttacataac cacttgtacc acttagagat gttatccatt taattatgtc
    13741 tctgtgaaca agaggcgcta cccagtacaa ctctccatct acattttgta tatgaaattt
    13801 tcctaatttg gatacactcc ctattgcagg aacttctcca agctttttgt cacctaattt
    13861 cattgccata tcttcatcta caagacgaat gtcatttaca ctgacagggc taatgtcatc
    13921 agtaaattta ctttcagtta cttttcccaa tagttctcta tatgattttg catgaagtac
    13981 aggtgtagaa gttataaagg gaactactac aatatacaca agcaaagcta ttgctattga
    14041 aaaattatat ttagccattt tagatgctct ttcatttcta tctaacatca tatctagtat
    14101 tcctgctaca acaaagaaaa ttattcctac tgaaaataaa gatgtaaaat ctaatcttag
    14161 tacaggtaat ttaataaatg cataaattat tacaattatt aatgcaactc catatgtttt
    14221 aagatatcgt ttcatttaat actcctctct aaaaaatgtt attattacta ttatatccat
    14281 atatttacaa aaaataaata tttatcatat ttattatata ttaagatatc tttatatatt
    14341 tatagttctg cttattcaac tatatttaac attatacgtg tatgtataaa tactttttat
    14401 acactgtttg tactatccat ccctcaccta aaggagtggg atttttgcac tattctgtat
    14461 tatatatatc caaaaatatt ataaaaatac aaatattagc agaaatttaa aaactgaaga
    14521 aagtattcta actaagattt acaaattctt aatcctatcg aagttaaact actatcttca
    14581 gcctaattat ctagcaatat ttttttatat gaaaaactct aaatcaagtt tttaaatgtt
    14641 tgcgattttt gatacataag aatgttgtct agaattatat tctgataata aaccttcata
    14701 ctctaaggtt aagccagata aattataaaa atcatttggc gttacttcaa atgcttgttt
    14761 atttaaaatg tctatctcac ttttttctaa catgtcagca acaaaatttg aacatacata
    14821 tttatattca gattgtctct gaatattcat tagtaaatat attaatgcca ttgtatcata
    14881 atagtaatct tctctataat cactaattaa ttttatattt ttatatagat tttcatattg
    14941 taaattatct acttctaacg aatataccct acacacagtg ttttttctta tagcatataa
    15001 tccttgattt atattttctt caacaaatcc tccaataaat ggattccttg gatttagcct
    15061 tccaaaagaa tacattggtt ttagattttc atttaatgat attgacacat gtgtatacag
    15121 ctttttactt atattcctta ttaagtaaga taatatagtt cctgtatatg ttgttacaat
    15181 atagacttta ttcatcattt ccccccaatc tagtaactaa ataatattat acacttacaa
    15241 tttaatatca taagatattc taacataata aatgtggttt tacaaatttt gattttaagg
    15301 tactttaata ttatataaaa tataataaaa atataaatat aagttttcta tatttttaac
    15361 caacagatgt tttcttcaaa ttaatcagat gatttagtat atcttgaacc tttccagagt
    15421 cctttaaata tcctaatgct ccatttattt gtgattttaa atctatttct acttccatat
    15481 ccaaatcaac atttcctttt tttatagata tatttttaag tgttattggg taaagataac
    15541 ttttttccaa agtaatctta tttccattaa cctcaaatgg aactttttta ttgtagttac
    15601 ttaatatttt ttctagcatt ttatcactta gtttaaattt tccaattttc acattctcaa
    15661 gacttacaac taagtttcca tcctttaaag taggtctcag ttcaagttca tattgactat
    15721 ttaccaaacc aaatacctta tatggtcctg ctacctttat tttaccattt ttcatttcaa
    15781 caaaattatt ttcaagttca ttaattccat gcttctgcat caatgtatat attaaatttt
    15841 taaactcttc ctctgaaaca gatatttttc caaccattct aagtggattt tttactacct
    15901 ccatagaatt tatatatgag ccttttgata aatattcttc atctatgatt ttattatttt
    15961 gtgaactaac atcatatctt tcttttggtg tcaagatata tacaacaatt cctgcaagta
    16021 ctaaaacaat aacaattaaa cttaacaaaa gctttgcaac ctttcccata atcgtctcct
    16081 ttacaaatct gtcatcagat ttaaaataaa tttagataac taacttcaca taatattctt
    16141 aacaatatac atactataat acagtctttt aaaaattaat tcaaccaatc taatgttttg
    16201 taaataaact gtaaaaacat gtttttagta taaaagttaa tattttgcat aatactctta
    16261 attacacctt tatttttttc taatcttgga ttaaaattaa tattatgcaa tacagtgtag
    16321 tgttctgtat tataatcaaa ttaaatagga ggtatagaat atgtcaaatg aaacgaactc
    16381 atctaacttt ataaagaata ttattataaa cgacttagaa acaggaaaac atgacagtat
    16441 cataactcgt tttccacctg agccaaacgg atatttacat attggtcatg caaaaagtat
    16501 atgtcttaat tttggattag ctaaggagtt taatggaaaa gctaatttaa gatttgatga
    16561 tacaaaccca ttaaaagagg atgttgaata tgtagagtcc ataaaagaag atgtaaagtg
    16621 gttaggattt gattggaacg agttaaattt tgcatctaac tactttgatg aaatgtataa
    16681 gagagcttta atactaataa aaaagggtaa agcttatgta tgtgatttga cacaagagga
    16741 aatgagagaa tatcgtggta ctctaactga accaggaaaa gaaagtcctc atagaaacag
    16801 aactattgaa gaaaaccttg atttatttga aagaatgaaa aatggtgaat ttaaagatgg
    16861 tgaaaaaaca ttaagagcca aaatagatat gtcttctcca aatataaacc ttagagaccc
    16921 aattatatat agaatatctc attcaacaca ccataataca ggagataaat ggtgcatata
    16981 tcctatgtat gcttttgctc atccaattga agatgcaatt gaaggaataa ctcactctat
    17041 atgtacttta gagtttgaag accaaagacc tctttatgat tggtttgtta aagaatgtga
    17101 gatggaaaat atacctagac aaatagaatt tgctagactt aatataaata atactgtcat
    17161 gagtaaaagg aaattaaaac aacttgtaga tgagggtatt gttgatggtt gggatgaccc
    17221 tcgtgttcct acaatatccg gaatcagacg taagggatat actgcagaag cattgcgcaa
    17281 tttctgtagt gaaatagggg tatcaaaagt taactctacg gtagatagtc aaatgcttga
    17341 ttatttccta agagaaaact tacaaccaaa ggctcctctt actatgggag ttttaagacc
    17401 tttaaaactt attataacta attacccaga agataagatt gagatgcttg aaatagagaa
    17461 taatgctaaa gatgaatctc aaggaaagag attagttcct ttctctagag aattgtatat
    17521 agaacaagat gattttatgg aagaacctgt taagaaatat tttagatttt tcccaggaaa
    17581 tgaagtccgt ttaaaaggtg cttattttgt aaaatgtact gatgtaatta aggatgaaaa
    17641 tggaaatgta gttgaaatac atggtactta tgaccctgaa actaagagtg gctctggctt
    17701 tacaggacgt aaggtaaaat ctacaataca ttgggttgat gctaaatcag ctataccatg
    17761 tgaatttaga ttatttgaac cattaattct tgatgatata cctgaaaatg aaggtaaaca
    17821 tttcttagaa caaataaatc ctaattcatt ggaaatatta caaggttttg ttgaacctac
    17881 acagataaaa gatgctaaac cttttgataa attccaattt gttagaaatg gattctttag
    17941 tatagataat aaatatacaa ctgatgaaaa atttgtattt aatagaatag ttcctctaaa
    18001 aagttctttt aaaccaggta aataatctta caatcaaaaa atgaagactt aaaatataaa
    18061 ttacttctac tataaataaa cttagttaga agtaattttt tatttgagtc ttcatcattt
    18121 actttaaatg attaactttt ttcactacag attcccatga cttctattcc atcttttatg
    18181 cattctatgt taagaggaaa atctggtcct ttttctccgt caatatctgt tataagtgaa
    18241 tcttcacatt ctatatgcac ctttttagtt ttaaagtata gtatttcatt tccattatat
    18301 tgatctaaat aatcacctct aagcacactt atcaaaactg gtattgattt tggtattggc
    18361 attcctttaa aaataattac atccaaatat ccatcatcta tttcagcctt atatgctaga
    18421 tttatatttc ctgctgtttt gccattaaaa atcaacataa gatacatatc tccatcataa
    18481 tacatttcct cagaagatac ttttatctta aatttcctca tatacaatgc ttcttctatt
    18541 ccctttatat aataagaaat tcttccaaat gaatttttta agtctgtgtt tatcctttgt
    18601 gatacatccg taaacatacc agcacttgca acatttataa agtacttatt attgactttc
    18661 cctatgtcta ttttctttgg ttttgaattt atgatttcat ttatagcttc ttttatatca
    18721 aatgaaattt gaagagcatt tgaaaaatca tttgcagttc ctgtaggcaa aattccaatt
    18781 ggaattttta tgtctaattc tttcattgca tttactacta aatctacagt accatcacca
    18841 ccagctatca atatgtgttc ataagaacta tcaacttcct taaatgcatt ctttacaggt
    18901 tctttaatag ttaatctata gggtatcaat aaataatttt tttcttgata taattctatg
    18961 atagtgtcta aatttgataa tatctttttt tcacctgaat taggattgta tataagtttt
    19021 acttttttca ttatttctcc ttactcattt tattctttta gtatccaaaa tcttcattta
    19081 aaaaaatcac atgtattctt tctggtgtta attgtctttt tataagtgta taactacagc
    19141 atattcttcc tggacttgaa cagtctatac atataccagt agtcttacat ccagtatttg
    19201 tacttaatct tttacaattg gctggagcag ctattgtttt tgttcttttt atagcttcat
    19261 ctacatcttt aactatttta tttactcctg ctataactat tactttatca ggtccatata
    19321 tcatagcagc aaccctattc ccattcccat ctaaattgta aagttctcca tcctctgtta
    19381 tcgcattagt acttgtaaaa taagcatctg cagaaaatga ctttctatat atttctttca
    19441 tttcttctgg tttgatatct tttgcatatc tatcaagaaa attatatttt tctcttataa
    19501 aatcaatcac tcctgtttca aataaggtca tagaacctcc acatcctact agagaacctg
    19561 ctttaactat ttcttcaatt tttttaagaa gctcttcttt gttacttact aaataccctt
    19621 tcatattatt gttttccaaa gattttattg ttttttctat tcttttttca ttaagccaat
    19681 ttaaattttg atccatttta acttccccct tatttttaat atgtacttaa ttatttataa
    19741 actttatttt ctttataagt ttattttagt acatctaaaa aaaaatacaa tataaaactt
    19801 aaatagtttt acataatttt ttccaaaata acttactcaa tcaaaaaagg aaagta
//
LOCUS       DACXMZ010000056        19798 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.056, whole genome shotgun sequence.
ACCESSION   DACXMZ010000056 DACXMZ010000000
VERSION     DACXMZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.056"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            185..1273
                     /locus_tag="JD016_07770"
     CDS             185..1273
                     /locus_tag="JD016_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HAU4999536.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            1301..2092
                     /locus_tag="JD016_07775"
     CDS             1301..2092
                     /locus_tag="JD016_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAU4999537.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(2207..2770)
                     /locus_tag="JD016_07780"
     CDS             complement(2207..2770)
                     /locus_tag="JD016_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAU4999538.1"
                     /translation="MYLMNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNM
                     VVPSIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPN
                     LSGKKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYF
                     NSTIPHVYENYDDEVCISVWAMTPPSF"
     gene            2957..4159
                     /locus_tag="JD016_07785"
     CDS             2957..4159
                     /locus_tag="JD016_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HAU4999539.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            4235..5215
                     /locus_tag="JD016_07790"
     CDS             4235..5215
                     /locus_tag="JD016_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAU4999540.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            5636..8680
                     /locus_tag="JD016_07795"
     CDS             5636..8680
                     /locus_tag="JD016_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HAU4999541.1"
                     /translation="MKKTLKRLCTGFLALATVVTALPTTPVHAESKQYWTESKERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASSRMSIDQISDVS
                     LSIEYVKQYTDRHSGISKNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNAGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFADKDCTKQLATLTTDKNGNTDVAEVTAGTVYIKELSAPAGYKVDKT
                     VYSLKVEAGKTATLNVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYTKDNLPAETTRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKAPNGYLLDGAYMQAGDKSEQIKGLYVTQITEDGDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKTIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGENHVVVTDDNGQFSTSAEWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSESNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFVADDEEMKVEISYTFNASALGGKTLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKEIEAEKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYMFTADSETMKVEVAFTFDATSL
                     NGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKPEEPEKPETPPTPEKPS
                     RPSDSPKTGDNTNLYGLLALLVTSGAGLAGIFFYKRRKMKKS"
     gene            8693..8971
                     /locus_tag="JD016_07800"
     CDS             8693..8971
                     /locus_tag="JD016_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999542.1"
                     /translation="MMKEKSRSPPKLSNGRLLLILACLLLTCKNTDSQGCVSIDFSLY
                     YLGDCWYFPNRQNKKSQSKNLSKIERNEVKHYGNEICRARYGTVFWNS"
     gene            8919..9245
                     /locus_tag="JD016_07805"
     CDS             8919..9245
                     /locus_tag="JD016_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 domain-containing protein"
                     /protein_id="HAU4999543.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPVFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYSIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            9261..9644
                     /locus_tag="JD016_07810"
     CDS             9261..9644
                     /locus_tag="JD016_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 domain-containing protein"
                     /protein_id="HAU4999544.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSSQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNAGSPQNHTPQGQQKK"
     gene            9737..10156
                     /locus_tag="JD016_07815"
     CDS             9737..10156
                     /locus_tag="JD016_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HAU4999545.1"
                     /translation="MMFEDLDKFIAENSEEILVTAGRTDDKISELETQLDIAFREEVK
                     IFIRKYGVLIGYGVEIVACGLNGHSRFVEDTLRFRKNGLEKKYIVIEGDSELAYCLNN
                     ETGEIVNWGLDNFEVYLVAESLESFVLAQFEEGKTNW"
     gene            10231..10473
                     /locus_tag="JD016_07820"
     CDS             10231..10473
                     /locus_tag="JD016_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999546.1"
                     /translation="MNIKEAIKMIQDEKLQGYNMNERKDDRENEVVLMRENDKWIVYA
                     TDERASKVTNSQDIYLDEEEALDDFIDRLRAFNRLR"
     gene            10582..10803
                     /locus_tag="JD016_07825"
     CDS             10582..10803
                     /locus_tag="JD016_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999547.1"
                     /translation="MEECEVKIYYKGFLCNLAPYRVMGEDRHALFPVTQSNDPIFYEE
                     FDEVHYGLWAKVLTDEEYQEIVDTVTKNE"
     gene            10840..11490
                     /locus_tag="JD016_07830"
     CDS             10840..11490
                     /locus_tag="JD016_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999548.1"
                     /translation="MIKRIWTFKRITLAIITFMCTIIFAISLQKSIMGTDKLQRDFGV
                     AFLFIVVLVCFLYFLYRLLIPKSFRCMTVVKKYLSFRELKDRINNESFSKVVIDEKKS
                     GKIEIYYSSKWIYADEVYIPRKLVLDLIEERKSLYSSFEKLSIATKNGENIIFAIVDI
                     EEAEKIINSLQEIFEEFTLDFNNMRKIQNRLLRKEIKQEFYKRVINKEDFLKESGL"
     gene            11701..13068
                     /locus_tag="JD016_07835"
     CDS             11701..13068
                     /locus_tag="JD016_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAU4999549.1"
                     /translation="MHKIWNKGHRIRASDKHLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFRLLENGLTLSPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQEIGRLEEKRT
                     ASNES"
     gene            complement(13074..13274)
                     /locus_tag="JD016_07840"
     CDS             complement(13074..13274)
                     /locus_tag="JD016_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999550.1"
                     /translation="MQVYIDLENLLKEKNISKNKVCEACKLQRTQLNNYCKNKVSRID
                     LTILAKLCDFLECSPNDILKLK"
     gene            13386..13796
                     /locus_tag="JD016_07845"
     CDS             13386..13796
                     /locus_tag="JD016_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999551.1"
                     /translation="MCKKRKIITITVLFVLGVAVSYLQWGREIDVAGSMGTSSENFHE
                     EHVTFTLNRLIITDKEKCAEEIIKKCRENDFTSILFSYDVAKPNALYGTVYRSRFSMK
                     GQKPLFHFRYTQTADTLGSYNIVDDPEAFTLVIE"
     gene            14035..15231
                     /locus_tag="JD016_07850"
     CDS             14035..15231
                     /locus_tag="JD016_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAU4999552.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFSTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LVDGGVMKRIDLAINDHTGILDIPELAEKCRKQEYIGKSRSYKFYQSGELIKHREHDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPA
                     KDLVES"
     gene            15244..15378
                     /locus_tag="JD016_07855"
     CDS             15244..15378
                     /locus_tag="JD016_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAU4999553.1"
                     /translation="MWVLLKDFLLVSMGMGIGVVLMCILNVGKEADYEMKQLEESEDN
                     "
     gene            15379..15600
                     /locus_tag="JD016_07860"
     CDS             15379..15600
                     /locus_tag="JD016_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999554.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            15804..17009
                     /locus_tag="JD016_07865"
     CDS             15804..17009
                     /locus_tag="JD016_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999555.1"
                     /translation="MMYGIIAVALIFVFANLPQYIRKQLTEKIADESSKAKENLQTSQ
                     LNFLTKIEWLKFYPLKKILLGVIWLVCLFYAVRGVFMTINDPRGVFIFLTVLFWLITI
                     FTARKIWRYIKLSYHCVPVLNHIKTKEELKESLQGECFEKVLFEHNILRKYFPVLISE
                     NWVVMNGRLFPRHGVEKIYYLHESPVWNYEQIRFIYSNGEEYLTPSDRQTVDELRQTE
                     ISNLLHKICPVVIEKAEDINTTSNKKDKSIIYWKMNYKGKFRRTLWMIPFVIILCILL
                     PLCLGSSWIIYDIIMIAILVWQLWYTYKKMKLEERTLNEIDENNNKKYTIAMKNIHMY
                     NYLLAIKDETHIYKAIIEAVPDENNDMMIWLDDFNFPQTEIEDIKKEIELYFKAKSIN
                     CIFKAGKRV"
     gene            17166..17462
                     /locus_tag="JD016_07870"
     CDS             17166..17462
                     /locus_tag="JD016_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU4999556.1"
                     /translation="MSDIFKDMQSKVGCEYISDLPSYKRKVWQEMKRLNPADYEERQL
                     EDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGCWWKREEQLAKKQYHTGSTCR"
     gene            17381..17884
                     /locus_tag="JD016_07875"
     CDS             17381..17884
                     /locus_tag="JD016_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAU4999557.1"
                     /translation="MQETRVLVETRGTTGEETISYWFDLPIDVAEFEEKLGIGAESQD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSSDMQEEYVSLLTVYENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQAYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            17902..18405
                     /locus_tag="JD016_07880"
     CDS             17902..18405
                     /locus_tag="JD016_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAU4999558.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            18515..18913
                     /locus_tag="JD016_07885"
     CDS             18515..18913
                     /locus_tag="JD016_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU4999559.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNDVPDKIY"
     gene            18891..>19798
                     /locus_tag="JD016_07890"
     CDS             18891..>19798
                     /locus_tag="JD016_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAU4999560.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGVAYEDYEYQLPKKKLQKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVR"
BASE COUNT         6924 a         2993 c         4074 g         5807 t
ORIGIN      
        1 tagataaaca taatttttct aagtgttatt tatttgtaga gctgaataaa agtgaaaatt
       61 cttatctaga atttattgat ataattaaaa aaacaagtat aaaagaatta actatagtat
      121 gttgtagcta aagtgtttta acaggataaa atatatcacg attttaagat aggagttgag
      181 tcatatgaga gattttagca cgtattctaa agatgatgtt atttttttat taaaagatat
      241 atcaaattta attgaagaag aaggaaataa agaaagagaa ttaaaaatac aaaatggtag
      301 acattattca gaaatgatac caatagagta tgaagttagt gaagattatc tgaatttata
      361 tcatgagaaa ctaagagaaa ataaagataa acttgcattt tctataggag ttatgtgtga
      421 aaaaataatt aaaaaaaatg gaaatgatgt agtactagtt tcacttgcta gagcaggaac
      481 acctattggt attttggcaa aaagatatat aagaagcaag tataatataa gtttacctca
      541 ctatacaatc tctataataa gagataaggg aatagatatg aatgcaattg aatatatagt
      601 aaagaatcat ccgagctcaa aaattcaatt tttagatgga tggactggta agggaactat
      661 atcaaaagaa cttgaaaaag catgtgatga acttaatgtt aaatttaaaa aatattttga
      721 ctcaacactt gctgtattag ctgaccctgc tggatattca ggactttatg gaattagaga
      781 agatttctta ataccaagtg catgtttaaa ttcaaccgtt tctggtttag taagtagaac
      841 tgtactaaga gaggatttaa ttggtaaaaa tgacttccat ggagctaagt tttatagaca
      901 tttaaaagat aaagatgaat ctatgaacta tatcgaaact atagaagaat gttttaaaaa
      961 tcaatttaag aatatatctg atgaagttga aaactgggaa agtgacatta taacaagaga
     1021 tggatacttt gatgttttaa atataaaaga aaagtataac ataacagata ttaactttat
     1081 caaaccaggt gtaggggaaa cgacaagggt tttacttaga agagttccgt ataagattct
     1141 tgttaaagac ttaaatgata aatctttaga ccatatattc atattagcta aggagaaaaa
     1201 tgttgaagtt gaacaaatgg atttaaaagc ctataaatgt tgtggaataa taaaaaatat
     1261 gaaagatatt taggaaatat attagacagg agaatattga atgattgttt tttcagattt
     1321 agacaggagt atcatttact ccaataaatt tttaaatgct gatagtaaat atgctaatat
     1381 agaaatttat agggaaaaag aaatatctta tatatcttta gatactataa atttaataaa
     1441 gcaaatacaa tattatggta tgtttatacc tacaactact agaacagtgg agcaatttaa
     1501 gcgtatagaa tttaataaat atgggattta ttttccttgg agtataactt caaatggagg
     1561 agttatactt aaagataatg aaatattgaa atcatggagt gaaaaaatag ataaactaaa
     1621 atctgattat gagcccattg aaagtatgat tcataaattt aaagactatt tgaatgtaga
     1681 tggaataact aattttaaag ttgcagaaga tacatttttt tatatagttg ttgatttatc
     1741 aagatttaat ttggattcaa tcaaagaata tacaaatata ttggaatcaa aaaattggaa
     1801 gttttatgtg agtggcagaa aaatatattt tataccaaaa gaaatttcaa aagaaaatgc
     1861 tataaaatac ttgactaaag aattaggaat agaatatttt tatgcagttg gagattctat
     1921 tatggactat ggtatgctta atatctcaaa taaatcctat gtattgaagc atggtgatat
     1981 aaataaaaat gaaatagaaa attcatttat atcaagttct ttcaatggaa tgagtggaac
     2041 tgaagagata ctatctaaca ttttagacga gaactgccta cttaatatat aatgactgat
     2101 atataaataa taaactagaa aaatctgaaa taaattatag taaagtttat catactaatt
     2161 tatttcagat ttttatcaat ttatatttaa gtaagaactc aatatgttaa aatgaaggtg
     2221 gagtcattgc ccagactgag atacatacct catcatcata attttcatac acatgtggta
     2281 tagtactgtt aaaataaaaa ctatctcctt cctccaatag atattcctta tttccaagtg
     2341 ttatcttcat tttacctttt attatatagc cacattcttc tccttcgtgt gtcacaaatt
     2401 cactgttttt ttgtgtatga ccatctagcg taacaagtat aaattctatt tttttcccag
     2461 ataaattagg aacaagaagt tcataaaatt ttgtagaatc attagttact attttttttc
     2521 gattattttt ctttactact atatcagagt cctctcttcc aatctcttca aaaaaataac
     2581 ctatatttat atcaagagct tttgatattt tccacattgc tacaatagat ggaacaacca
     2641 tattacgctc aatttgactt attaagcctg tacttaaatc tgaatttttt gctaaagtag
     2701 agatacttat atttttagat tttctaagag atctaatttt gcttccaaca tctatattca
     2761 ttagatacaa accccctcag acatgattat aacatataat ttataaagat taataaaata
     2821 aaaaaaaagt tttacaatac aagtattttt tatgctatat tgaattttct gaaaagatgc
     2881 attataatag tattaatatt caatatattg aatattgata acgatatttt aatttaatga
     2941 aatggaggta attaagatga agagttttgt aagtcctgat aatatttggg tattatgggc
     3001 agttgtaact ggatgggcag catttagtat ttatttggaa caaaagtaca attgggcatc
     3061 aaaagtgtca ggagcaataa tagctcttgt aggagcaatg ttattatcaa atctaaatat
     3121 tataccagta gagtctgttg tatatgacca agtatgggga tatgttgttc ctttagccat
     3181 agctttattg ttatatcaat gtaacattaa aaagatatgg aaagaaagtg gaagattgct
     3241 cattatattt cttgtaggtt ctgtaggaac tgtacttgga gctatgattg gatttttagc
     3301 attaaaaaat gtagtaccag atttaaatat tgttgcagct atgatgacag gttcttatat
     3361 aggtggaaat gtaaactttg ctgctatgtc aggggcgttt gatgcgccag gagaattagt
     3421 atcagcaaca gttgtagcag ataatctttt aatggcatta tatttctttg tcttaatagc
     3481 tataccatca attggatttt ttagaaaaca ttttaaacat ccacatgtag atgaaatgga
     3541 aagtataggt ataactgaag gagaaacagt agcagcaaat tattggggaa gaaaagaaat
     3601 atctttaaaa gatatagcaa tagccattgg tagcgctttt ataattgtcg cagtatctgt
     3661 agaactatca acttggttta aagctataat accattatca aatccttttt tagcaatgtt
     3721 aaatacttta tttggaaatc aatatctaat aataactact ttgactatgt tgtttgccac
     3781 atttatgcct aatttctttg gtgatataaa aggagcacag gaattgggta catttttaat
     3841 atatatattc tttgttgtag taggtgttcc tgcttcaata acattaataa ttcaaaaatc
     3901 accattacta cttttatact gcggaataat ggtactaata aacatgctag ttacatttat
     3961 agttgcaaaa atatttaagt ttagtttaga agaagctatt cttgcatcta atgcaaatat
     4021 tggtggtcct acaacagcag cagctatggc aatatcaaaa ggatggtcta agctagtagg
     4081 gccaatactt atagttggaa catttggata catagttggt aattattttg gtcttttagt
     4141 aggaaatatt ttgatataat tttacgtgaa aaaaagttgt tttttagaaa gatttttatt
     4201 tattagaaag atttttattt aattggaggt tgttatgatt ttcgatggac attcagatac
     4261 ttgggcagat gtaacaataa gaaggcttaa cggggaaaaa gatgtattta gaagaatcca
     4321 tcttgataat tttaaaagag caaatataga aggagctatt tttgtaatat ggatagaccc
     4381 accatatgat aaagagcctg aaaaaagagt taaacaaatt ataagctcta taaaagatga
     4441 aattgaagat atgaaagatg ttgtaaacat agtaaaaaaa tattcagata tagataaagg
     4501 tataagtgaa aacaaaataa atattttaat tggtcttgaa ggattaagcc atattggaag
     4561 aaatatagat ttactagatt atttttataa agaagttggt gcaagacatg catcattaac
     4621 ttggaatgaa cagaatgaac ttgctacagg atgtttaggg gatgaaaatc gtggtttaac
     4681 tgaaataggt aaaaaagcag tcaataaaat ggaaaaattg ggcatgatag tagatgtgtc
     4741 acatttaaat gaaaaatcat tttgggattt aatgtctgtt gcaagtaagc cagttatagc
     4801 ttctcattct aatgctagga gtttatgtga tgctaaaaga aatcttacag ataatcaatt
     4861 aaaagaaatt tcaaatacag gtggattagt tggaataaat agttttagag gatttactaa
     4921 tagtaatcct aagaagcaag atttaataca tttggttgac catatagact atatggtaaa
     4981 tcttataggt atagaacatg tatcttttgg ttttgatttt agtgattact tagggaaaga
     5041 gactataggt tcttttactg gaaatgctag tgtgccaagt gtagatggtc ttgaaagcac
     5101 acatacagca cagaacatag tagtagagct tgaaaataga ggttataaaa agaaagatat
     5161 agagttaatc tctagtggta atttttatag gctctttaaa gatatactaa aataatataa
     5221 taaagaactt aaatttataa aatagtatat ttatacaaat aatatctaca tatttaaatt
     5281 ggcgttcata atttattaaa ttaaatttta atgttactag aagtttcaat tctagtaaca
     5341 tttttttgac taaaaacaca ataaaaatct gattatccgc taaaacacag tatctatcag
     5401 cattttcaag gaaataaaca catcaattta ctacttattt actactaacg aatgagcctt
     5461 gatacgcaag ttttcctaga tatgcaaaga tatggtacag cacatcagta ttgaaacaag
     5521 aaaaaattga ataacttata gactatggaa cgcctaacat gacgttcctt ttctattttc
     5581 agccgttctt attggacggc tattttatta cccgaatgaa aggagcatta gatttatgaa
     5641 aaagacattg aaacgattgt gtacgggctt cttagctctt gcaactgtcg ttactgcttt
     5701 accgactaca cccgttcatg cagaaagtaa gcaatactgg acggagtcaa aagagcgtgt
     5761 cggtatcgtt gaaaaagtaa tgaatgacgg ttcgattggt tctaccttta atgagggaca
     5821 tttaactgtt gagggcgagg acgcttactg tattgacatt aacacagact ttaagaatgg
     5881 ttacaagacc agagctgacg caagctcacg catgagtatc gaccagataa gcgacgtttc
     5941 cttatctatc gaatatgtaa aacagtacac agacagacac agcggaataa gcaaaaatca
     6001 cgcttacctt ttaagacagc ttgtagtctg gcagagattg agcgtacatc ttggctggca
     6061 atgtgataac gtgcgagctt cttatgacga aataccaaag gctacgcagg acgaagtttt
     6121 cgctggtgca aaagcctttg tcaaagaaaa taaaggacgc tatgaatgtg gcggttatat
     6181 ctactctggc gaggggcagg aattgggaca attttgggcg aagctgaatg ccggaaatgc
     6241 gaaactgcaa aagacttcca gtaataccag cattaccgat ggtaacggga attactctat
     6301 cgctggtgcg acctacggtg tctttgctga taaggactgc acgaaacagc ttgccaccct
     6361 tacgactgat aaaaacggaa atacagacgt tgcagaggta acggctggca cagtttatat
     6421 caaggaatta tccgcaccag caggatataa agtagataaa actgtatatt ctttaaaggt
     6481 tgaagctgga aagacagcga ccttgaatgt atctgataca ccaaaagtaa cagacacttt
     6541 gattgagctt ttcaagattg atatggaaac acagaaagac aatccgcagg ggaacgcttc
     6601 tctagcaggt gcggaattta catggaagta ttatgcaggc ttctatacta aagacaatct
     6661 tcctgccgaa actactcgta catgggttac aaagacaatc gctgaaacag acagcgacgg
     6721 gacaacccac tacatcacaa aattagcgga cgcatacaag gtatctggcg acagcttcta
     6781 tatgcaggac ggtaaagcgg ttcttccact tgggacacta accgttgaag aaacaaaagc
     6841 tccaaacggc tacttgttag acggtgcata tatgcaggct ggcgataagt ccgaacagat
     6901 taagggctta tatgtaacac agattaccga ggacggcgac cttgccgtac tatctggaag
     6961 taaccagttt tccgtatcag acaaggttat ccgtggcggt gtcaaaattc agaaacgaga
     7021 tttagaaacg ggcgatacaa agccacaagg aagtgccact ttgaaagata ctgcctttga
     7081 catcatttcc ttaaatgata attctgtttt agttgaggga aagctataca agaaaaatga
     7141 agtggtaaag acaattcgta cagatattga gggtgtcgct tctacttctg ctgacctttt
     7201 accttatgga aaattccgta tcgttgaaag tgaagctccc gacggttact taactgacgg
     7261 tgcaaaaacg attgatttta caatcacaga aaatggaaaa atcgtggact taaccgacga
     7321 agcccattct atctacaatc agattaagcg aggggatatt gagggtgtaa aaatcggtgc
     7381 aggcacacac aagcgtcttg ctgatgttcc gtttaggatc acaagcaaga cgacgggcga
     7441 aaatcatgta gtggtaactg atgataacgg gcagttctcc acttctgctg aatgggcttc
     7501 acataagcat aataccaacg caggcaagac cagcgaggac ggtgtgtggt ttgggacttc
     7561 tgaaccagac gacagcaagg gtgcgttacc ttatgatacc tacatcatcg aggaattacg
     7621 ctctgaaagc aacaaaggct ttgaacttat cccacctttt gaaatcgtgg tatcaagaaa
     7681 taaccttgtg attgatttag gaacactgac tgatgaatac gaaaaagaaa tttctatcca
     7741 taccacagcg accagcaagg acggcgaaaa gactatcctt gcaggaaaag aggttacaat
     7801 cgttgatact gtaaaattag acggacttac aaaaggtaca aagtatcagt tgaaaggctg
     7861 gcagatgtta aaagaagaaa atgccgagct tatcattgac gggaaacgtg tagaaaacga
     7921 ttataccttt gtcgctgatg atgaggaaat gaaagtggaa atttcctata cattcaatgc
     7981 gtctgcttta ggtggcaaaa cccttgttac ctttgaagaa ttgtatgatt taagcaatcc
     8041 agatgaaccc gtgaaagttg cagaacataa ggacattgag gacgacgggc aaacggtact
     8101 tatcacagag cgtatcatca agatacatac taccgctacc gataaggacg gcaacaaaga
     8161 gattgaagct gaaaaagacg ttacaatcat tgataccgta accttagagg gcttagaagt
     8221 cggtacacag tacaaacttg tgggctggca gatgttgaaa gaagaaaatg cagagcttct
     8281 tatcaatgga aagcgtgtgg aaagtgatta tatgtttacc gctgacagcg aaactatgaa
     8341 agtggaagtt gcctttactt ttgacgctac ttctcttaac ggaaaacagc ttgtaacttt
     8401 tgaagaattg tacgatttaa gcaatccaga tgagccgaag aaagttaccg agcataagga
     8461 tattgaggat aagggacaga cgattacttt taaggaaaag ccagaagaac cagaaaaacc
     8521 agaaacacca ccgacaccag aaaagcctag cagacctagc gacagtccca aaacgggcga
     8581 taacaccaac ctttacggac tgcttgcact tcttgtgact tctggtgctg ggctggcagg
     8641 tatcttcttc tataaacgcc gtaaaatgaa gaaatcataa ggcggtaacg gtatgatgaa
     8701 agaaaaatca cgctctccgc cgaagctgtc aaacggcaga cttttgctta ttctggcttg
     8761 tctgctactt acctgcaaga atacggatag tcaagggtgc gtaagcattg attttagcct
     8821 ttactatctg ggggattgct ggtacttccc aaaccgccag aataagaaat cacaatcaaa
     8881 aaacttatca aaaatagaaa gaaacgaggt aaaacactat ggaaatgaaa tatgtcgtgc
     8941 cagatatggc acagtctttt ggaactcttg aatttgcagg cgaaagcgag ccagtctttg
     9001 aaagagataa aaataaccgc agggtcttag ccagacgaag ctataatctt tattctgacg
     9061 tacagaaagg cgaaaatgtt gtggtagaaa ttcccgtgca ggctggcgaa aagcatttca
     9121 agtatgagca gaaagtaaaa cttgtcaatc cgaagttata cggcagaggt tactcaatcg
     9181 gggatatggg acataccgat tatgtgttag ttgctgacga tattgtagca gttgaagaaa
     9241 agtaaaggag tgaagaattt atgagattat caaacggatt tgttattgac aaagagaaaa
     9301 catttggaga attaaaattt acagcggtgc gtgatgtgtt cttgcaaaat gaggacggga
     9361 caccgagtag ccagcttaaa aagcgtatct atgacttgaa gtgcagtctg cacggtggaa
     9421 ttatccccgt gtctgtaccg ccagaagtac cgttaaggga atttccgtac aatgcagtcg
     9481 tggagcttgt caatcccgta gccgatacag tatcacgaaa gacctatacg ggtgcagatg
     9541 tggactggta tgtaaaggca gaggatattg tgttgaagaa taaaggcaat cagaacgcag
     9601 gcagtccaca gaaccataca ccgcagggac agcagaaaaa ataggcttgc tgaatatatt
     9661 ttcattgtag cggatatatg gaactgttat aatatggata tgaagaaagc gacaaattag
     9721 aatttatgag gtggatataa tgtttgaaga tttggataag tttatagcag aaaattcaga
     9781 ggaaattctt gttactgctg ggcgaacaga tgataaaata tctgaattgg aaacacagtt
     9841 ggatattgca tttagagaag aagttaagat atttatacgg aaatatggag ttttgattgg
     9901 atatggtgtt gaaattgtag cgtgtggtct taatggacat tctcgatttg ttgaagatac
     9961 acttcgcttt agaaaaaatg gtttggaaaa gaaatatatt gtcatcgaag gagatagtga
    10021 gttggcgtat tgtttgaaca acgaaacggg tgagattgtc aactggggat tagacaattt
    10081 tgaagtgtat cttgtagccg aaagtttaga atcttttgta ttggcacaat ttgaagaagg
    10141 taagacaaat tggtgataaa atcccagttt gtttaactag aaagaaatag aaaaattaga
    10201 agttgtagta caaaaatagg agcatgaaac atgaacataa aagaagcaat aaaaatgata
    10261 caggacgaga aattacaagg ttataatatg aatgaaagga aagatgatag agaaaatgaa
    10321 gttgtgttaa tgcgtgagaa tgataagtgg attgtttatg caacagatga acgggctagt
    10381 aaggttacaa attcacaaga tatttattta gatgaggaag aagccttaga tgattttatt
    10441 gatagattaa gagcttttaa tagattaagg tagctatggg agaattaaaa tgacctctaa
    10501 aaagatattt agtaattttc ttttaaatag acaactccca gttcgtttaa ctaaaaagaa
    10561 atagaaaaat tagaattgta tgtggaggaa tgcgaagtga aaatatatta caaaggcttt
    10621 ttatgcaatc ttgcccccta tcgtgtgatg ggcgaagata gacatgcatt atttcctgtt
    10681 acccagtcga atgatcctat attttatgaa gaatttgatg aagttcacta tggattatgg
    10741 gcgaaagtat taacagatga agaatatcaa gaaatcgttg atactgttac gaaaaatgaa
    10801 tagacaactt agaatttatt aaaaaggttg gagaagaaaa tgattaaaag aatatggaca
    10861 tttaaaagga ttacattagc aataattaca tttatgtgta ctataatttt tgcaatctca
    10921 ttgcaaaaaa gtattatggg aacagataaa ttacaaagag attttggagt cgcatttctt
    10981 tttattgttg tgcttgtttg tttcctttac tttttatata gactattaat acctaaaagt
    11041 tttaggtgta tgacagtagt aaaaaaatat ttatctttta gagagttgaa agaccgaatt
    11101 aataatgaat cattttcaaa agtcgttatt gacgagaaga aatctggaaa gattgaaata
    11161 tattattcat ctaaatggat ttatgctgat gaagtttata taccaagaaa attagttttg
    11221 gatttgatag aagaacgcaa atctttatat tcatcgtttg aaaaattatc aatagcaaca
    11281 aaaaatggag aaaatataat atttgccata gttgatattg aagaagcaga aaaaatcata
    11341 aattctttgc aagaaatttt tgaagagttt acattggatt ttaataatat gagaaaaatt
    11401 caaaatagac ttttaagaaa agaaataaag caagaatttt ataaacgagt tataaataaa
    11461 gaagactttt tgaaagaaag cggtttgtag agcatgttaa aataggaaca atagttgcaa
    11521 taatattagg tttattcata tttacgtatg gaattgattt gttattaaaa cttttgacta
    11581 tgttattggg tcgataatat atttcttgtt tgcgaattgt ctgacaactt catatcacac
    11641 ataaagcagt tagtcttagg attgactgct tttttgtacc cagaaaggag tgattgactt
    11701 atgcataaga tttggaataa aggacaccgt atcagagcca gcgacaagca ccttgtctac
    11761 cacttttcca tagggacgct tttgtttgta ttcgtggcgg ttctcctgct actgaatatc
    11821 aaacagctca tgcgtaccga ttgggagcat ttcagattgt tagagaacgg cttgacgctc
    11881 tccccttaca acttcataac catactgata gcgactggtg tttgtgcatt ggtcgctttt
    11941 ctgtattacc gtttttgtta tgacagtttc aagaagctcc tgcaccgaca aaagctggcg
    12001 agaatgatac tggaaaataa gtggtatgaa gccgataccg tacaagacag cggttttttt
    12061 actgacctgc aaagcagatc aagggaaaaa atcgtctggt ttccaaagat ttattatcaa
    12121 atggaaaagg gactgcttca tatccgctgt gaaatcacgc tggggaaata tcaagaccag
    12181 cttttacggt tagaggataa attggaaagt ggcttgtatt gcgagctgac cgacaagaca
    12241 ctgcatgacg gctatatcga atataccctg ctttatgata tgatagcgaa ccgcattacc
    12301 attgatgaag tacgggcaga aaacggctgt cttagactga tgaaaaatct tgtctgggaa
    12361 tatgacgcac tcccacacgc tctgattgct ggtggtacgg gtggcggtaa aacttatttt
    12421 ctgctgacac tcattgaagc cttactgcat accaacgctg tcctttatat cttagacccg
    12481 aagaacgctg accttgcgga cttaggaaca gttatgggaa atgtgtatca caccaaagaa
    12541 gaaatgatag attgcgtcaa tgccttttat gagggtatgg tacagcgaag tgaggaaatg
    12601 aagcgacacc cgaactataa gacgggcgaa aactatgcct atctgggact tccaccctgc
    12661 tttcttatct ttgatgaata tgtggcgttt tttgaaatgc tggggacgaa agaaagcgtg
    12721 agcttactta gccagttaaa gaaaatcgtt atgttaggac ggcaagcagg ttatttcctt
    12781 atcgttgcct gccagcgtcc agacgcaaag tatttttcag acggtatcag agataacttc
    12841 aatttccgtg tgggacttgg acgtatcagc gaattaggtt acggtatgct gttcggttca
    12901 gatgtgaaaa aacagttttt tcagaagcgt atcaaggggc gtggctattg tgatgtggga
    12961 acaagtgtta taagtgaatt ttacacgcct ttagttccga aaggacatga ttttctacaa
    13021 gaaatcggca ggctggaaga aaaaaggact gcaagcaacg aaagctgaca gtcctacttc
    13081 aatttcaata tgtcatttgg ggaacattcc agaaaatcac agagctttgc aaggattgtg
    13141 aggtcaattc tggaaacctt gttcttgcaa tagttattaa gctgtgtccg ctgtaattta
    13201 cacgcttcac agactttgtt tttactgata ttcttctctt tcaaaagatt ttctaaatca
    13261 atatatacct gcatacgctg tacctcgtct tttctttgaa ttataagata caactgtatt
    13321 gataaataag atgtattacc gtagactgta ttataataca ccctacaaga aaggacagta
    13381 acagtatgtg caaaaagaga aaaatcataa caatcacggt actttttgtg ctgggagtgg
    13441 cggtttccta tctgcaatgg ggacgtgaaa ttgatgtagc aggaagtatg ggtacttcat
    13501 cagaaaattt ccatgaagaa cacgttacct ttacgcttaa ccgtcttatc ataacagaca
    13561 aagaaaagtg tgcagaggaa atcatcaaaa aatgcaggga gaatgatttt acttctatcc
    13621 tgttcagcta tgatgtggca aagccaaacg ctctatatgg tacggtgtac cgttcccgtt
    13681 tttccatgaa aggacaaaag cctttgtttc acttccgata tacacagaca gcggacacgc
    13741 tcggttcata caacattgtt gatgatccag aagcgttcac gctggtaatc gaataacgca
    13801 aggcaggacg ggacggcgac gtgcgaagcg aaaggcgacg gcacggacta gccgttgctg
    13861 gtgtggcata agccacgcca gcaggtaaaa cccctcggta tctaacagag gggtacgttt
    13921 gcaaatagac aatagacaaa aaacatagga aacataaggc ttctggcatg atttcctagc
    13981 aaaattcagt tgtgaagtcg ccttaaaaaa cttcacactt tacagattgg gggtatggtt
    14041 ctgaatgaag aacaatggat aaaagaatta cgggagaaac ggattgctta cggtatctca
    14101 caaggcaggc tggcggtggc ttctggtatc acaagggaat atctcaacaa gatagaaagc
    14161 ggaaaaatga agccgtcaaa ggaacttctg gaaactttgc ataaagaact ggcaaggttc
    14221 aatccagaag caccgcttac tatgctgttt gattatgtga aaattcgttt ttccacgctg
    14281 gatatacagc acatcatcaa agatatatta aaactgaata tcaactatat gctccatgaa
    14341 gattacggac attacagtta tacggagcat tattctttag gggacatctt tatctatacg
    14401 tcggctgacg aagaaaaagg tgtcctttta gagttaaagg ggcgtggttg caggcagttt
    14461 gaaagttacc tgttagcaca acaaagaagc tggtatgact ttctcatgga cgcactcgta
    14521 gacggtggcg tgatgaagcg tatcgacctt gctatcaacg accatacggg cattttagat
    14581 attccagagc ttgcggaaaa atgcagaaaa caggaatata ttggaaagtc cagaagttac
    14641 aagttttacc agtcgggcga gcttatcaag cacagagagc atgacagaga atatatggga
    14701 cgtacccttt atcttggttc gctgaaatca gatgtatatt tctgtatcta tgaaaaggac
    14761 tatgagcagt atgtcaagtt gggaacacct ctggaagaag ccgacattat caaccgtttt
    14821 gagatacggc ttcgcaatga acgagcctat tatgcagtac gagatttgct gacctattat
    14881 gacgcagagc agaccgcctt ttctatcatc aaccagtatg tgcggtttgt tgatgaagaa
    14941 cccgacaagc gaaaaaatga ctggaaactc aatgaccgct gggcttggtt tattggcgat
    15001 aacagacaga gcttgaagct gacgacaaag ccagagcctt acaccttaga ccgcacattg
    15061 cgttgggtac aaaggcaggt agcaccgacc ttgaaaatgc tgaaaaagat tgataaggga
    15121 aacggtacag actacatgga aacaatcgaa cagcaggcaa agctcacaga aaagcatgaa
    15181 atgataatca aacagcagac gacccctgca aaagatttgg tggaaagtta ggagtgataa
    15241 aaaatgtggg tattattaaa agacttcctg ctggtatcta tgggaatggg tattggcgta
    15301 gtcttgatgt gtattttgaa tgttggcaaa gaagctgact atgaaatgaa acaattagaa
    15361 gaaagcgagg acaattaaat gaatttcgga caaaatttat atcaatggtt tttatcaaac
    15421 gcacagagct tagtgcttat ggcgattgtg gttatcggta tctacttagg gttcaagcgt
    15481 gagttttcaa agctcattgg cttcttggta gttgccttga ttgctgtcgg cttggtattc
    15541 aatgctggtg gtgtgaaaga tgtactgtta gagctgttca ataagattat cggtgcataa
    15601 aattttattg tggtgctgat atgagaatgg tataatgtgg atatgaagaa aacggcaaat
    15661 ttgaatttgt ttgatgttag taatacaaga atttgaagtt tcagaggtaa agataattga
    15721 tatcttcaga taaagggaaa tataattatt aaaatagcaa aatcaaaaga agttaaaata
    15781 aatttataaa ggatggatga gaaatgatgt atggtattat cgctgttgca ctgatatttg
    15841 tatttgctaa tttaccccaa tatattagaa aacagctaac agaaaaaatc gctgatgaaa
    15901 gcagtaaagc taaagaaaat ttacaaactt cacaattaaa ttttttaaca aagatagaat
    15961 ggcttaaatt ctatccattg aaaaaaattt tactaggagt aatttggctt gtttgcctat
    16021 tttatgctgt aagaggggtc tttatgacta ttaacgatcc tcgtggtgta tttatattcc
    16081 tcacagtttt attttggctt atcactattt ttacagcacg caagatatgg cgatatatta
    16141 aattatctta tcactgcgta cctgtattaa atcatatcaa aacaaaggag gaactaaagg
    16201 aatctctaca aggtgaatgt tttgaaaaag tattgtttga gcacaatata ttacgaaaat
    16261 atttccctgt ccttattagt gaaaactggg tagttatgaa cggtcgtctt tttccaagac
    16321 acggagttga aaaaatatat tatttacacg aaagccctgt ttggaattat gaacaaatta
    16381 gatttatata ttctaatggt gaagaatacc taactcctag tgacagacaa actgtagatg
    16441 aacttcgtca aacagaaata tcgaatttat tacataaaat atgtccggta gttattgaaa
    16501 aagcagaaga tataaatact acatcaaata aaaaagataa atcaattatc tattggaaaa
    16561 tgaattataa aggtaaattt agaagaacat tatggatgat accatttgtt attatcttat
    16621 gtattttact gccactatgc ttaggaagtt cttggattat atacgatata attatgattg
    16681 caattttagt atggcagctt tggtatactt ataaaaaaat gaagcttgaa gaaagaactt
    16741 taaatgagat agatgaaaac aataacaaaa aatatacgat agctatgaaa aacattcata
    16801 tgtacaatta tcttcttgca ataaaagatg aaacgcatat ctacaaagcc atcattgaag
    16861 ccgtacccga cgaaaataat gacatgatga tttggttaga tgatttcaat tttccacaaa
    16921 cagagattga ggacataaaa aaagaaatag aactgtactt taaagcaaaa agtattaatt
    16981 gcatatttaa agctggtaaa agagtataga cgaagtgttc agacacggcg gtggaaaaat
    17041 ctcttagact acaaaagtca agagcaaaaa tttcagtttg acaatttcat atccatatac
    17101 acaaagcagt tagtcttatg attgactgct tttttcttac caaaatttca gattggagtg
    17161 atgaaatgag cgatattttt aaggatatgc agtcaaaagt cggctgtgaa tacatttccg
    17221 acctgccctc ttacaaacgt aaggtgtggc aggaaatgaa acggctgaac cctgccgatt
    17281 atgaagaaag acagttagaa gatttttcaa aatatgtgtt tggtatgtcg taccagacct
    17341 taaaagatgt aatgaaacaa cagaaaggac gtgaggaaca atgcaggaaa caagggtgct
    17401 ggtggaaacg agaggaacaa ctggcgaaga aacaatatca tactggttcg acttgccgat
    17461 agatgttgcc gaatttgaag aaaagttagg tatcggtgca gaaagtcaag attatcgtat
    17521 tatcgaaaag gtgttgcctt atgctgatga agtccacgaa catacaagcg tgtatcagct
    17581 caacgaatta gattttatgt accgccagct ttcaagtgat atgcaggaag aatatgtttc
    17641 actacttacg gtgtatgaaa atttagaagc actttatatt tgcaggaacg tgattacggt
    17701 ttatcccgac tgcaaaagca tgattgatgt tgctaggcaa aagctgatga acgacccgac
    17761 gttcaaacat ttatccgagg actgtcaagc ctactatttt gactttgaag cctatgcttc
    17821 tcacttgcag gaacacggga aatttttagt aacagaacac ggtatctttg aactgccaga
    17881 gtaggaaatg aggtggtgct tatgattgat gatatggcgg tttatattgc taatcttggc
    17941 aaatacaacg agggctattt agtcggtgct tggttcacat ttcctattga cgaggaagat
    18001 gtaaaagaaa aaatcggctt gaatgaacag tacgaggaat acgcaatcca tgataccgat
    18061 aacttcccga ttgcgattgg cgagtatgtt tccattgaag aactcaatga gatgtatgaa
    18121 atgatagagg aacttcccga ctatatcgta gaatgtctgg acgaatttat cagccactac
    18181 gggacgctgg aagaagtcgt ggaacataag gacgatattt attattatcc cgactgtgaa
    18241 accatgacag atgttgccta ctactacata gacgaattgc aggcactagg ggatattcca
    18301 cccagcttac agaactacat tgactatgaa gcctacgggc gagatttgga tatgggcggt
    18361 tgcttcattg aaacaagccg aggtatgtgc gagataccat attaacgctg gaagatcagc
    18421 gacaagcatt gttgctactg acagcgtatt tctcttttaa gggacagtct gaaacatggc
    18481 tgtccttttc tattttgaaa ggagctgaaa gaacttgaaa aagattaaaa gctacacggg
    18541 tatctggaac gtggaaaaag tcttatatgc aatcaatgac tttaacttac cctttcccgt
    18601 tacttttaca cagattacat ggtttgtgat tacagaattt atcatcattc tgtttgggga
    18661 tattccccca ctttccatga ttgagggagc atttctcaaa tacttcggta ttcccgttgc
    18721 tctcacttgg tttatgtcgc agaaaacctt tgacggaaag aagccgtaca gctttttgaa
    18781 atcacagata acctatgccc tgcgacctaa aatcacttat gcaggaaaag ccgtaaaact
    18841 gcataagcag atattgaatg aaacaatcac ggcagtaagg agtgtgaacg atgttcccga
    18901 taaaatatat tgacaataac cttgtctgga acaaggacaa tgaggtattt gcctattatg
    18961 agctgatacc gtataactat tcttttctat ccgcagagca gaaatttatc gtgcatgaca
    19021 gctttcgcca gcttatcgca cagtcccgtg agggtaaaat tcatgcgttg cagattgcca
    19081 cagaaagctc tattagaagt atgcaggagc agtcaaaaaa gctggtaacg gggaaacttc
    19141 gtgaggtggc agttcagaag atagacgaac agaccgaagc gttggtatct atgattgggg
    19201 acaatcaagt ggactaccgc ttttttcttg gctttaagct catggttacg gaagaacagc
    19261 tcaatctgaa gaacatcaaa aaatcggcat ggctgacgtt cacggaattt ctccatgaag
    19321 tgaaccacac gctgatgaac gattttgttt ccatgccgaa tgatgaaatc aaccgttaca
    19381 tgaaaatgga aaagttactg gaaaataaaa tctcccgtcg ctttaaggtg cgtcgcttgg
    19441 aaatcaatga ttttgggtat ctcatggaac atctttacgg cagggacggt gtcgcctatg
    19501 aagattatga gtaccagcta ccaaagaaga aattgcagaa agaaacgctg ataaaatact
    19561 acgaccttat ccgtccgaca aggtgtgtga ttgaggaaag ccagcggtat ttacgattgg
    19621 aacatgagga caaggaaagc tatgtgtcct actttaccgt caatgcgatt gtaggggagc
    19681 ttgattttcc gtcctctgaa atcttctatt tccagcaaca gcaattcaca ttccccgttg
    19741 atacttctat gaatgtagaa atcgtggaaa atcgaaaagc attaacaacc gtccgcaa
//
LOCUS       DACXMZ010000057        19533 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.057, whole genome shotgun sequence.
ACCESSION   DACXMZ010000057 DACXMZ010000000
VERSION     DACXMZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.057"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            748..1404
                     /locus_tag="JD016_07895"
     CDS             748..1404
                     /locus_tag="JD016_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999561.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1912..3183)
                     /locus_tag="JD016_07900"
     CDS             complement(1912..3183)
                     /locus_tag="JD016_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAU4999562.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            3428..4144
                     /locus_tag="JD016_07905"
     CDS             3428..4144
                     /locus_tag="JD016_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HAU4999563.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            4223..5533
                     /locus_tag="JD016_07910"
     CDS             4223..5533
                     /locus_tag="JD016_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HAU4999564.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            5633..6169
                     /gene="lepB"
                     /locus_tag="JD016_07915"
     CDS             5633..6169
                     /gene="lepB"
                     /locus_tag="JD016_07915"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAU4999565.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            6335..6871
                     /gene="lepB"
                     /locus_tag="JD016_07920"
     CDS             6335..6871
                     /gene="lepB"
                     /locus_tag="JD016_07920"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAU4999566.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6881..7636
                     /locus_tag="JD016_07925"
     CDS             6881..7636
                     /locus_tag="JD016_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HAU4999567.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            7710..9377
                     /locus_tag="JD016_07930"
     CDS             7710..9377
                     /locus_tag="JD016_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HAU4999568.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            10247..11176
                     /gene="glsA"
                     /locus_tag="JD016_07935"
     CDS             10247..11176
                     /gene="glsA"
                     /locus_tag="JD016_07935"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAU4999569.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            11274..11837
                     /locus_tag="JD016_07940"
     CDS             11274..11837
                     /locus_tag="JD016_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999570.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            12325..13161
                     /locus_tag="JD016_07945"
     CDS             12325..13161
                     /locus_tag="JD016_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAU4999571.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            13460..14455
                     /locus_tag="JD016_07950"
     CDS             13460..14455
                     /locus_tag="JD016_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAU4999572.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(14712..15116)
                     /locus_tag="JD016_07955"
     CDS             complement(14712..15116)
                     /locus_tag="JD016_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAU4999573.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            15277..15467
                     /locus_tag="JD016_07960"
                     /pseudo
     CDS             15277..15467
                     /locus_tag="JD016_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15957..16871
                     /gene="queG"
                     /locus_tag="JD016_07965"
     CDS             15957..16871
                     /gene="queG"
                     /locus_tag="JD016_07965"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAU4999574.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRNIIKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLNYDEIKNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            16977..19214
                     /locus_tag="JD016_07970"
     CDS             16977..19214
                     /locus_tag="JD016_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAU4999575.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            19412..>19533
                     /locus_tag="JD016_07975"
     CDS             19412..>19533
                     /locus_tag="JD016_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAU4999576.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKG"
BASE COUNT         7661 a         1817 c         3655 g         6400 t
ORIGIN      
        1 atagagtaat aatatgatta taaatttatt gattaatttt attattattt ttaagggggg
       61 agagtatttg tagttataaa gcatattggg aataatgaaa aaggttaaaa acatattact
      121 ataaagtata gggggtaggt taaaagagta atcataaaat aaatatacag ataatagtag
      181 ataagaatac cagaaaaaaa tagatagtta aagggttagg gtaaaagagt aattgtaaaa
      241 taaatatatg gatgatggtg gataagaata tcagaaaaaa tagatagtta aagggttaga
      301 gtgaaagagt aatcgtaaaa taaatatatg gatgatgtgg ataagaatag taagaaaaaa
      361 tagatagtta aagggttagg gtgaaagagt aatcgtaaaa taaatatata gataatggta
      421 gataagaata tcagaaaaaa tagataatta aagggttagg gtgaaagagt aatcgtaaaa
      481 taaatatatg gatgatggta gataagaata tcagaaaaaa atagataatt aaagggttag
      541 ggtgaaagag taatcgtaaa ataaatatat agataatggt ggatagagta gtaagaaaat
      601 aaatagataa agggatagaa taaatttaaa tatggaaaat gaatatctaa taataagtga
      661 ttaataaata attgtagata gattgtaaat taaataaatc tttaatagaa aggagaacta
      721 tttatatatt aaatagtatt tataaatatg aaaaaaggcg attttatatg gagtggagtt
      781 cttatttgta ttattgctgt cttagcaata ccaagtagta gagaagtatt tatggaagca
      841 acaggtgcac atccatatat tggaggtttt ttaaagtttg ctgtattagc tacaatggga
      901 gatttacttg gtgctagaat tttgtataag gattggaaaa taccagtagg agtattttat
      961 agagcaatag tttggggaat aataggtgta gttaccacat atggaatgtc aatctactca
     1021 tcaggtgtag gtcaagcaca agtagatggt atgcttccat ttgagggaat agcttttgca
     1081 aatgctctat ttaagagtgc aactatgaat atactaatgt caccaactgt atttttattc
     1141 cataaggtta tggactgttt tatagataaa aaatatgagg ttggaaaggg gattaaggta
     1201 aaacttaaag atgtggtaaa tattgtggac tggaatgctt accttggttt tagtgtttta
     1261 aaaactatac catttttctg gataccatgt catacaattg tattcttatt ccctgctgag
     1321 tatagagtaa ttgcttctgc atttgcatct atagctttag gtctaatatt ggctttagct
     1381 aataagtcta aagttgcagt ttaggttcac tgatgaagat aatagtatta tttaaattag
     1441 gaataacttt ttaaattaat attatttgta tttataataa ataataattt atataagaag
     1501 taatattcta agtgatggat agtgttttaa ataataagaa gtaatattct aagtgataga
     1561 cagtgtttta aataataagg agtaatactc taagtgatag atagtgtttt aaataataag
     1621 tgagatttta aataaaaagt aggtttttcg ttagaatagc gttttattaa acttaaaaat
     1681 aaatataata ttttaaagaa atctaagttg ctaagcactg tttttaagaa gcttaaaatc
     1741 ccccatttaa agttttaaga ttctaaaaaa acaagttata actaaaaaat ggctgttaag
     1801 actacaatac ttataagtaa tacttatggt atatattagt tcataacagc catttttatt
     1861 ttaaattaat aattttattc aataatttga gtttatgttc taaatcaagc tttaaattaa
     1921 aggatttaaa gaagctattc tagttgtagc tacataaact ctggatattt cataccatgt
     1981 tttaaaatct actactccgc tttgaggtaa accaaagatt ccttggaata tcttaacagc
     2041 gttttctgta tctgttccat agataccatc ctctataact tttggtacag ctggataaga
     2101 attagatata gcatttaact gattttgtat ggttctaaca tactgtccag aagaaccaac
     2161 ttgtaatgta gtaccaggga atgacactgg tacaccactt actataggtg ctctttcaaa
     2221 tactatttca tctccataga aatatcttag tatactctca tagtcatatc cttggtcacc
     2281 taagtcttta ctgccccatt gagtcatttg gtcaggacat tgggattttt gtccatcaca
     2341 atattgtgct aatagtggct gtcttgatgt aggaggtctt tttataaaag tattaaaaat
     2401 agcatctaca actacgttaa ttggttcaaa taagtttcta ttatttataa acttatggtc
     2461 gtatgctgtt gttgaagtta ttgtaaagtt atatccttta tttctatacc attcagtaaa
     2521 gactctattt aaagtaaatg atattatagc tattacattt gcatagattg tctgttcagg
     2581 ccaagttgaa tatatttcag atgacgctat gtttttaatg tactctttaa atggaatcca
     2641 atagtttgga gctgatgaat cttctggcaa accatcatga actactataa attcaggcac
     2701 tacagggtta tctaatacaa cgaaaccagt aggaggtggt aatggtttta agttactttc
     2761 tggaatctta ggaggataag taccatatag agtatgttct cctatgtcaa aaatcaactc
     2821 actttgtctt gagaaagaac gtttacttct tgttctagga gacatgggaa ctccttgtct
     2881 tgcttcaatt gtagctaaca actgtgttcc ttctatgaca actgtctcat atccatctgc
     2941 tatggcttcc actatatact ggctataagg tctaacatca gatggttgta gagagtaatc
     3001 tacatctggt gctggtaata ctaaaccagt cacttgacca gattcattac ttcttaggtt
     3061 ttggtataaa gttgatgagc tttggctacc attagacatt gaatatatgt ttactactgc
     3121 attttgaata ggtctattgt tagtagcatc aattatgctt actgttaaga aaccatcttg
     3181 catcaaatca ccctttcttt aaatgaattt agttccctta tatttcaaga tatgaatata
     3241 gcttgaaatt tgttaaataa tatagatata aatttaaatt tttttgagta tttgagtata
     3301 tttgtattta aataatttaa aataatttaa tgtatatttt agtatgtatt taaaacacga
     3361 ataagttaaa aataagtaga tgatataaat cataaataaa tcaaaaattt tgaaaggaaa
     3421 ataatctatg aagctagatt tatttttatt agcaatagtt cctatattga tagggatgtt
     3481 ttggataagg tctaaagata gatattgtag agaaccccta atccatctaa ttaaattttt
     3541 tttaataggt gcttttctaa gtgttataat tatactttta gaaaatttac tgatgaagtt
     3601 taatgtattt gaagggtatt cagaactaat atatgtatca tttgtagttg caggacttgt
     3661 agaagaaggt gtaaaagctc tgatactgat tcctgcccta attaaagaaa aacactttac
     3721 tgaaaagtta gatggtatta tttactctgt atttttagct ttaggatttg caacaattga
     3781 aaatatggtt tatatttttt ctgaaagtag aaatctagca ctgcaagttg gtattaatag
     3841 ggcagttatc tctataccag ctcatgtaat gtttgcaatt actatgggct actatatttc
     3901 taagtataaa tttgagggaa ataaaaataa aagacgtgag tatttgttta tggcagtgtt
     3961 aattccaatt ttattacatg gagtttttga tttcatattg atgatagaat atagatgggc
     4021 tattatatta cttatagttt atgtaataat tttgtggaag ataaatcttg ataaacttga
     4081 gaaatatatg aatcactcta aaaaagtgtt tttcggtaat cttaggaaaa agaagaagaa
     4141 ataaaattta aaggaaagac ataaataatt aaagtataat aaatgacaca caagtaaagc
     4201 agattgaagg gggattatag atttgacaga attaccaaag gaatttttag aagatattga
     4261 agaaatacta aaagatgaat atgatgattt tataaaaagt tatgaggaaa gtaagactac
     4321 tgggcttaga ataaatactt taaaaattga caagaaaaaa ttatttgatt tggagttgtt
     4381 tgatttgaat caaataccat gggcagaaga aggtttttat tatgatgata aaattgacag
     4441 acctggtaga aatccactgc atgaggctgg agcatactat ttgcaagagc cttctgcaat
     4501 gagtgtaata ccaaaggcag atattaaaga gggtgaaaaa gttctcgata tgtgtgctgc
     4561 tcctggtgga aaatctacat atatactatc taagctaaac gatacaggtt tattggtttc
     4621 taatgaaatt aatgctagta gaattaaggc tctaggggaa aatttagagc gatttggagc
     4681 gagaaattgt attataacaa acaccgattc taatagctta agaaagacgt ttaatgggta
     4741 ttttgataag gttattatag atgcaccttg ttctgggcaa ggtatgttta ggaaggatga
     4801 ggttgccata tcagattgga gttatgcaaa ggtagtagaa tgtcaatcca tccaaaaaga
     4861 gattataaga gatggatata aaatgttaaa gaaggatggc attttaattt actcaacttg
     4921 tactttttca agggaggaaa atgagcatgt tataaatgag tttatcagtg aatatccaca
     4981 tgcagaactt attgaaatgg aaagattatg gcctcataag ataaagggag aaggtcattt
     5041 tgttgccaaa ataaaaaaat tagaagaaga agaatgtaat gtcaaagaat taaaaataaa
     5101 aaatttagat aaagaaataa aggattatag ggagtttgaa aagaaatttt taaaggttga
     5161 tttttatagt aataaaaaaa ataaatttta tttaagagga gaaaatttgt acctacttcc
     5221 tgaaatatat ccagatacga aaaaactaaa agtattgaga tatggtttgc atttaggggt
     5281 tttaaagaag aatagatttg agccttctca tgcactatct cattacttaa aagttgaaga
     5341 tgtaaaaaat gttgaaaact tcagtgtaca gagtgaatca attcttaaat atttaaaagg
     5401 ggatgttgta aattctaatg atagtagagg atgggttttg gtttcggttg aaggaatacc
     5461 tttaggttgg ggaaaagaat cttctggtgt aattaagaat cattatccta aaggtcttag
     5521 aaaagtattt taaactatta gaaaaaagaa gtataatatt tatattgtag gtttatggta
     5581 gattattttg ctgtagattg acttaatatt taacacagag gagacgattt tagtgggtga
     5641 agcagttaaa aaagaagttg tagaatggat aaaagtgatt gtcatagctc ttgttttggc
     5701 atttgcaata actcgtttta tagtgccaac aatagtcaaa ggagaatcaa tgtatcctac
     5761 attagttgaa cgtgattatt tgatagttaa cagaattgcg tacaaggtag gagagccaaa
     5821 atacaaagat ataatagtat tcaaaaccga cttaacagag gaaaatggaa agaaaaaaga
     5881 tttagtaaaa agagttatcg gggttcctgg tgaccatgta aaaatacaag actccaaggt
     5941 atatgtaaat gataagttgt tagatgagac ttcctatata cataataatc gtactgatgg
     6001 agatattgat atcgtagttc cagaaggaaa attatttgca atgggagata atagagaaaa
     6061 aagtttagat agtagatacg atgaggttgg attggtcgac gagcatacca ttttaggaaa
     6121 ggttctagtc agattgtatc cattttctaa gataggaact attgactaaa catataaaat
     6181 ttggagcaat cgctgattag ctgaaatcac cagaccatac acaatagttg tctatataaa
     6241 aaagacaaca ccttatttag tagacttatc taaaagatag tgtttaagag acattatcaa
     6301 taaagataat ataactataa aggagaattt gtgaatgaat gaaactatta aagaagagat
     6361 tgtagagtgg ataaaaataa ttattactgc actttttttt gcatttatta taactcgttt
     6421 tataaaacca acattagtaa atggagaatc aatgtaccca acacttaaat cacatgatta
     6481 tttggtagca aacaggatga catataagtt atcagaacca aaatgtggag atataatgat
     6541 atttaagact gatttattac aagagaatgg aaggaaaaaa gagcttgtaa aaagggttat
     6601 aggtgttcct ggtgaccatc taaaaattaa ggattctaag gtttatataa atggtaagtt
     6661 attaaatgaa gtttcatata tacatgataa ttatactgaa ggcgatattg atatggtgat
     6721 tcctaaggga aaagtatttg cgatgggaga caatagagaa gttagtttag acagtagata
     6781 taaagaagtg ggattagtag atgaagaaaa tattaaagga aaagttattt taagagtatt
     6841 tccttttaca gatataggta tttttgagta ggtgatttgt atggcagttg gtatagcgtc
     6901 cttattgttt aatattgagg aggctttaaa catttgtgag agtataaagc aaataactca
     6961 tttagagatt ggaattgaca atatatctga atgtagtgag ttatgtaaat ataaagagag
     7021 aatttctaag ttaggattat ctatagggat acatttgcct atggaattaa atacttgcga
     7081 aaacatagag tatatacgaa attcatggat aaattttatt gagaagatag aatttgaact
     7141 aaaaggattt gatttaaggt attttaacct tcatttggga tatgttatga caaatagagt
     7201 tagaaagaat agagacaaat atttaggaaa tagtgtggat tttttagata aattaaatac
     7261 aaattcatat gtatgtatag agaatactta ttcaaaaggt ggggattttt caaatattgg
     7321 aaatatatct tatgattttg aatatatatt taagaggatt aaaaattcta agatttgttt
     7381 ttgttacgat actggacact gtttaattga tgaggatgct tatgtaaaga acttaaagga
     7441 taaaataaga ctgatacact taagtgataa tgatggtatc aatgatacac atgttggtat
     7501 aggaagagga attttatctg aagaggggat taaagaggtc ttaacattag atgcagaata
     7561 tttagtatta gaaataaatt acgaagacat tgaagatact ataagtaaat tgaataatat
     7621 tgtaggagag ggttaaatac ctctctttat cttgttgata tgcctcatta attaaaattt
     7681 atattcatca aaaggaagga gatgttgaag tgaaaaaaat aaacttaaaa atagaaggaa
     7741 aagataagga atattcatta gaagaaaatt cccctggaat cagattggga gatatagcaa
     7801 aagaattttg tgatgagcat aagggttata taacacttgc agttgtagac aataaactaa
     7861 aagagctaaa ttgtagagtt aagaaggatt gtgagataaa ttttttagat actactaatg
     7921 aagatggaga gagagtttac tttagagtca tgtcatttat atttgtaatg gcatgtagag
     7981 aaatcttttg ggatagcaga gttacaatag aacattcact atcagatggg ctatattgtg
     8041 aagttcatat agatagaaag cttaaagaag cagatgttga aaatataaaa aacaagatga
     8101 aagagatagt aaacaacgat tatgttatag aaaaaattga agttacaaga aatgaagcta
     8161 tttctatctt tgaaaataat gaaatgtatg aaaaggcaga gcttttgaaa tacaaagagt
     8221 atgatagtgc taaagtatat aagtgtagaa actatataga ccatttttat ggttatatgt
     8281 tgccatcaac tggttatata aaatcatttg atattaagct ttataatgga ggcgttataa
     8341 ttttaggacc tagtgaagaa gataagactc ttcctatgaa atttgtacct caacctaagc
     8401 tatcaagtgt atattatgaa gcagaagaat ggtcaagact tatgggaatt gacaaggtag
     8461 tatctcttaa taaaataata gaaaacaatg aatatggaga tataataagg acatttgaag
     8521 cattgcatga aaagaagtta tcccaaatag cggatatgat aaaggatggc aataagagag
     8581 tagttttaat agcagctcca tcttcatctg gaaaaacaag ttttgctcac agattatcaa
     8641 tacatcttag agtaaataat ttaaatccta tttcaatttc tttagatgat tattttatag
     8701 atagaaaaca tactccttta gatgaatatg gaaattatga ttttgaatca atttatgcaa
     8761 tagatttaga gaagtttaac ttagacttaa agaaactttt agctggtgaa gaaattgatg
     8821 atataagatt caattttaaa gaaggtaaga gagaatacac tggtaaaaag ataaagatag
     8881 attcaaatca gccaattata ttagaaggta tacatgggct aaatcctatt ttaacatcat
     8941 ctataccaga tgaagataaa tttaagatat acataagtgc ccttactcaa ataaatttgg
     9001 atgaccataa tcgaatccct actgctgatt tgagaatgat aagaagaatt gttagagact
     9061 ataattttag aggatatagt gctgataata ctattttaca atgggcatca gttagaagag
     9121 gagaaaagaa gaatatattc ccataccaag aggaggcaga tgctatattt aactctgctt
     9181 gtgtatatga attggcagtt ttaaaacgat ttgcgaaacc tttgttggaa gaaataaagg
     9241 aagataatcc tgcacatatt gaagctacta gattactaaa gtttttgcag tattttgttg
     9301 aattggacga tacttctgac ataccaggta tttcaattct tagggaattt attggaggaa
     9361 gtaaaatagt tgattaatgt gtgtactata gattgtttta tgaagattaa atggcatagc
     9421 aagtagtgtg attggtttat gttaagtata ttaagtgtaa agtggagaat taaattgata
     9481 cagattttaa agtttgagtt gatatataaa aaggcttgtt ttatactttt gtatgaagca
     9541 agctttttat tttaaatttt acttttttgt ttaatatttt gttagtttat gtgattattt
     9601 tttaaaagag taaaagagta aaagagtaaa agagtaaaag agtaaaagag taaaagagta
     9661 aaagagtaaa aggtgttggg ttagaaattt ttggtgttat aaaaatttaa attactggat
     9721 tattagattg ctggattgct aaattactag gttgttaaat tattgaatta ttaggttgtt
     9781 gaattactaa attactaggt tgttaagtta ctggattatt tagatagtta gattactagg
     9841 ttgttaaatt attgaattat tagattgttg aattactaaa ttactaggtt gttaagttac
     9901 tggattatta gatagttaga ttactaggtt gttaaattat tgaattgtta gattgttgaa
     9961 ttactaaatt actaggttgt taagttactg gattattaga tagttagatt attagattgc
    10021 taaattatta ggttgttgaa ttactggatt attagattac taaattgcta ggttgttaaa
    10081 ttattgaatt attaaattat taagctcttt aaactattta aattgtttga aaaatgaatt
    10141 gattcttgta gaaaataatt ttaaagtata atttttttta acattgtttt attatagtat
    10201 aaacttgtgc taaaatagaa tatataacat aatataagag gtgcatatga atacgttaga
    10261 tgagacatta ttaaaagaaa ttataagttc taataagaag tatactaatt atggacaagt
    10321 cgctagttac ataccagaat taaaaaacgc tagaagaaat gatttaggaa tttgtatcat
    10381 agatagcgaa aataatcttt atagtgctgg aaactgtagc acaaagttta ctatacaaag
    10441 tatttcaaaa ccaattgtgc ttgctatggc acttatggat aatgattggg aagacgtttt
    10501 ttcaaacgtt ggaatggagc caagtgggga cccatttaat tcaataatga aacttgaaat
    10561 taatgacaca aaaaaaccct gtaatccaat gataaatgca ggggctatag taaccacttc
    10621 ccttataaat gggagttgtt tagaagaaaa agaagaaaga atgcttagtt tctttagaaa
    10681 gttagcaaaa aatgataata taggaataaa ctatgatgtc tataagtctg aaaaaatgac
    10741 tggagataga aacagagcta tggcttactt attgaaaagt gatggattta tcagagggaa
    10801 tgtagaagat gttctagacc tttattttaa acaatgctca attgagatag attctgtaga
    10861 tttagcgaga attggaataa atcttgcaaa ttatggagta gatatagaaa atggtgagca
    10921 tttaatgagt gaaatggttt ctaggattgt caagacattt atgatgactt gtggaatgta
    10981 tgatgcttct ggggaatttg ctattaaagt tggcattcct gcaaaatctg gagttggggg
    11041 tggtataatg gcttctgtgc cgggacgcat ggggattggt gtttatggac ctgcgcttga
    11101 caaaaaaggt aacagcgtcg caggagttaa agttttagag gagttgtcaa ataaactcaa
    11161 attaaatatt ttttaaaata ttaagaaagg atttttttga caacaacata taatttaata
    11221 tataattaaa ttgtaaagaa atatgaatta aataaatttt ttaggagggg tcaatgaaag
    11281 aggtattggt attacgagat ttggagtgca taaaggctat tgcgcatcca cgtagaatag
    11341 atattctaaa agcatttgac aaatcgccat tgtcagcaaa acaattatct caattattgg
    11401 aagaacctca tgcaaaaata aactaccata taaaaatgtt atacaaagtt ggtattttag
    11461 aattagttga ggaaaaaata aagtcgggga ttgtggaaaa gtactattat ccaagtgcaa
    11521 aaaatgtggt tatagggaat aggatactta atttttcttt agacaatggg gaagaaaaag
    11581 aagaattgta catatcaaaa tttgaaaata tgagtgaagt tttttataag gcagcagagg
    11641 aagatgtttt agaaaatgaa aatatagttg attatcatga tatttctctt actcatgatg
    11701 aattagttga acttagtgac actatgaagt caaagataga tgaaatttta aataaaagac
    11761 aacacaatgt tgaaggttct aagtatgata tagctatggt tatagttcct actcttgagg
    11821 aggaatgtcc tagttagaga attaattatg gattaattaa agatataata ttagagattg
    11881 aacatcaaaa tttacttaat aatcaaaaaa tagtttttgg tatgggtaaa acaatgattt
    11941 ttaatctcta tttttttgtt ttcttatacc tattgtattt gtaaataata tgtatagtgt
    12001 aagatgtata cactatctaa gaagtgctta gataatatac aaagtatgta agattataat
    12061 atttgaaatt gattaaaatg ggtataatta aagtgtaaat attaaaatta aaaattaata
    12121 taaattaaat caatgctaga aatatgttaa aagaggaatg agattagttg ggatagaaca
    12181 tcccttagag cttacgaatt tttagtgaaa aagtactatc aaataggaat ctccatcttc
    12241 atatgttggc taaatgtagt taaggtggag tagttcaaaa ttaaaattga gtacaagtcg
    12301 tagtacaaat aaaaggagat taatatgatt aaattaggaa cacatctttc aatagcaaaa
    12361 ggctttgcta atgcggctga gacagctgta tatattggtg caaatacatt tcaatttttt
    12421 agtagaaatc caagaggagg aaatgctaaa gaatttgatg aaaaagatat aattaaattt
    12481 caagaaatta gaaaagaaaa taattttgca ccactgcttg cacatgcacc atatactatg
    12541 aatctgggtg gtacaaagga tgatgtatac gaatttgcaa caagggtgat agatgaggat
    12601 ataagaagga tggactcatt aggaattgaa tacatgtgtt ttcatcctgg aagtcatgta
    12661 ggtggtggtg tagattttgg tatagataga atagtaaaag gattgaacaa tgccataaag
    12721 ggtgatgaaa atataactat acttcttgaa actatgtcgg gaaagggaac tgaaataggt
    12781 tttaaatttg agcaattaaa aagtataata gacaaaattg agcataaaga aaaaataggt
    12841 gtgtgtcttg atacatgtca tattttttca gctggttatg atatagtgaa tgatttagat
    12901 ggggttttgg aagaatttga taaggttata ggcatagaaa gattgaaaac tgtccatcta
    12961 aatgatagta tgatgccatt tggagacaaa aaagacagac atgctcctat aggggaagga
    13021 aagataggat taaaagcatt aattgatttt atggaacatc ctagtctaaa acatttacca
    13081 tttttcttag aaactccttt tgatgatgaa ggtcacaaga gagagcttaa aatgataaaa
    13141 gaaatactat ctagtaaata aacctttatt aaaaatatgt gaaatactcc accctcaaca
    13201 taattagggt ggagatacaa tcatgaaatg tgataattta aattaatgga tagtaataac
    13261 aaaaattgta tacaaattaa ctgggtataa agtatgatta tataaagtat gattatatta
    13321 tgaatagatt gatataaaat ataattatat tatgaatagt atgtaaatat attgcaaaga
    13381 ttctcataat ttgttgtata attaaaggtg acatatttat attatataaa aaattattaa
    13441 aagttaggtg gggaaagata tgagaaattt aggcaatact aacatgaaaa taaagagagt
    13501 tggctttggg ggcattccaa tacaaaggat tactcaagat gatactaact tagttataaa
    13561 tgagttagaa aaacaaggaa taaactttat tgacagtgca agagggtata caataagtga
    13621 ggaagcaatc ggaattgcca ttgaaggaaa aagagataaa tttttcttag ctactaaaag
    13681 tatgtccaga gattatgatt caatgaagag agatgttgaa ataagtttaa ataattttaa
    13741 aactgatttt atagatttat atcaattcca taatgttaaa gaagaggagt atgataatct
    13801 ttttaaagat aaaatggctt atagtgccct tttagaggca aaggagcaag gtaagataaa
    13861 acatattggt ataactagtc acaatttaaa tactatagaa aaagctatag aagatggaaa
    13921 atttgacact atacaattcc cttacaatat agttgaagga caagcagatg aagtgtttaa
    13981 aaaagctcat gaaaaaggaa ttgggataat agtaatgaaa cctttagctg gaggtgcttt
    14041 agacaatgca acactggcta ttaaatatat actatctaaa gattacatag atgtagttat
    14101 accaggtatg gagagtgttg aacaggttag acaaaatgtc gctgtacttg aaaatcttgt
    14161 gctagatgaa aaagataata aggaaattga agaaataaga aattctttag gtaaaaaatt
    14221 ctgtagaaga tgcgaatatt gtatgccttg tgctgtagga ataaatatac cattgagttt
    14281 tttatgtgaa ggttattata ctagatatgg attaaaagag tgggctaaag aaaaatatga
    14341 ggttatggat gttaaaccta ctgagtgtat tgactgtggg ctttgcgaga gtagatgtcc
    14401 atatgaactt cctataagag aaatgcttaa gactgttgtt gaaaagctag gatagatttt
    14461 atttattgaa atcggtgtat taaatttact ccgatttctt tttattagat gtggtcttat
    14521 atacaaatat taaatttaca tagttatagc tattatataa atttaatatt tgtttaatac
    14581 ttattaaata tttgcttaat gctttttaaa aacatttgtt tgagtataga cataacacaa
    14641 ttattattgt tgtattagta tagtttcaaa tactggagta tcaatataat gtcgaataac
    14701 acataaaatc tttatttttc aaaaatatta taggatatat cgtatttaga tactggataa
    14761 agttcaattt caaccctagg gttatcttta tctataaaat caaatagaag tactggctta
    14821 agttgtttat cattttttat tattccactt ttttcaatac catcacaaat actttttgga
    14881 taattatgta aatcccccat tctcttattt gggaagaata atttcaagac tgttattaga
    14941 ttttcttcaa cagtttcccc ttgatactgt tggtttataa aacctgcaat catgttttcg
    15001 tatgcaagat acttagaata ttttcccttt gaaggcatcc atgcttgacc atttatgtta
    15061 tgaagcttaa aattagattt actaataggt ctacctggaa ttgttatttt aatcaaattt
    15121 ttcaccatcc ttatacagta tttattataa tctgtatatg atttaaaagc aaattgttat
    15181 ttaaagtgtt tttacagaac ttgtttgtaa aattttccac tttagatgta gtaatataag
    15241 ataagtattt tgcttacaaa ttttaaaatt tgatatatga tgggcatatt catattagac
    15301 tattataaga ccttcataat attaataagt ataagtttag taaagagagt aaaaagaaat
    15361 ccatataagt caagtaaaga cttaataaat ggcaatgtaa atgggatatt aattataaga
    15421 aaaaatcaat ctttttgctt aactatagaa aaaatgtcaa aatataacta atgatatttg
    15481 aagttttaga gtttatatta aaaagtaaga aggttaagtg ggtaaaaaag gttgaataga
    15541 ttaaaaaggc taggtatcta aaagagttaa gtaggtaaaa aagttaaata agtaaatgaa
    15601 aaaggctaag tatctaaaag agttagtagg taaaaaagtt aaataagtaa atgaaaaagg
    15661 ctaagtatct aaaagagtta gtaggtaaaa aactaaatag gtaaaaagat gaagtatcta
    15721 aaagagttag taggtaaaaa attaaatagg taaaaagacg aagtacctaa aagagttagt
    15781 aggtaaaaaa attaaatagc taaaaagacg aagtatctaa aagagttaag caggtaagaa
    15841 aattgaatag ctaaaaagat taagtattta aaagagttag tagctagaaa aggttaaaaa
    15901 gactaaaaaa attaaataaa tttaaaaagt caattagatt aaaaaggaat aattttatgg
    15961 ataataagga attaaaagca ttttgtaatt taataggatt aaactgtgta ggagtagcag
    16021 gggtagataa gtatgataat ttagaaaaga tacttaagga cagacaacaa aaaggttatt
    16081 taactggtat ggaagaacca ataattgaga acagaattaa cccaagaaat ataattaagg
    16141 atgctaactc tataatagtc tgtgcatttc catatcatat aaatagacaa gaaaagaata
    16201 aaaattcaaa tttatcaaaa tattgtcatg gaaaggatta tcacattgta gtaaaggact
    16261 ttttacaaca aatttgtgat tatatttcta aaacagtaac agattttaaa tacaagttat
    16321 ttgctgataa tggaccatta gttgacaggt atttagctta tttatcagga ataggatatt
    16381 ttggaataaa taataatatc ataactgatg aatatggttc ttacgtattt atagggtata
    16441 tagtaaataa ttataaattt gaagaagata aaccactaga aaaaagttgt tttaaatgtg
    16501 gtaagtgtgt aaaatattgt ccgggaaatg ctatattggg aaattatgat atgaacccca
    16561 aaaaatgttt atcatatata actcaaaaaa aaggagattt gtcagagaaa gaaaaagttg
    16621 ttttaaagaa tggcaaaaag gtatttggat gcgatatttg tcaagaagta tgtccacata
    16681 atgttgaaat accaacaact catattttgg aatttaaaga aaatataata gattatttga
    16741 attatgatga gataaaaaat atttcaaata aagaatttaa aaggagatat ggagatagag
    16801 cttttagctg gagagggaga aatataataa agagaaatat ggaaataatt ttagataagt
    16861 atgatgacta ataataagag tctgtttcat atactttaat gtgtatttac tttaacactt
    16921 gttgtgtaaa agtcatatta tatcattaaa gttaaatatg gggaggtggg ggaatattga
    16981 aatataataa tacatctgaa ataaatccac tagattatat attgggtcaa gaaagagctg
    17041 tagaagctat ggaattaggg ttgaaaataa ataatccagc gtacaacata tatatagctg
    17101 gtgaacctgg aactggtaaa agtacttatg ccttaaaggt tttaaatgag tatgcatcca
    17161 aaaaaaatac gcataaagat tggtgttata tatataattt tgaaaaccct agagagccta
    17221 ttatagttga attagaaaag gggtttggaa gagaacttaa gaaagatatg gagaaattaa
    17281 ttgaaagtct tttagaagat tttaaaaatg cgtttgaaag tgaaaatttt gaaatagaaa
    17341 aaaataaact tctggatgaa tatgaaatag aaaaagatat gcttttgaag caaattaaga
    17401 aatatggaga agaaaaagga tttaaattaa aacagagtaa aatgggaata gtttttatcc
    17461 ctttgaaaga agaggaagat gaaagtgatg agtctgatga agagttttac aaagctaaaa
    17521 gagaactaga aaatatggcg atacaggtcg tatataaaat tagaaactta gaagaaatag
    17581 ctgaaaaagc agtgttagag cttgaagaag agatagctaa acttgtagta gaacctcata
    17641 tcaagagatt gtgtgaaaaa tatgaaaatt atgataagat tcaaacttat ctggaaaata
    17701 taaagaaaga tataatagaa tatatgtatt tattctattt agatgaagag gagttaaaag
    17761 ataagtatga taaagagcat tttattaaat ataaaattaa cctatttgta gacaatggaa
    17821 gctcaaagaa taaagaatca gcacctgttg tagtagagat aaacccaagt ccagcaaatc
    17881 tatttggaaa ggcagagtat gattatgcaa atggaaatat aaagactgat ttcacaaaac
    17941 tattatcagg agcatttcat agggctaatg gtggttattt agtactttat gcagaccaat
    18001 tactaaaata tacaatgtct tgggaaattc ttaaaaagac tttgcagact aaaaaagtaa
    18061 tattggaaac acaaacagca attaaaccag aaaatatgcc attggatgtt aaattagtac
    18121 tgattggaag ccactatata tatgatattt tatatagata tgatgaagat tttgaaaaat
    18181 attttaaagt atttgtagac tttgatagtg aaatggataa aaatgaagat aatgaagaag
    18241 gaatagcaag atttatagca taccaatgtg ataagaataa tttaaggcat tttactaaat
    18301 cagcagttga agaggttatt aaatttagca ctagattatc tggtgattta gaaaaattat
    18361 caactaagtt taataaaata atggaagttg taatagaagg aagtgcttat gcagagttta
    18421 gaaattcgga gtatacagag cagtgtgatg ttaaaaaagc aataagtgaa aagagaaaaa
    18481 gaatcaatag agtggaaaca catatggatg aatcaattga aaatggattt actctaatag
    18541 aaacagaggg aagaaaagta ggtgtgataa atggtttgtc agtcttaagt acaggagaat
    18601 actcttttgg tagagcttca aggattacag cgaccactag cccaggaagt aaaggcatag
    18661 taaatataga aagagaagta aatatgagtg gctctataca taacaaagga gtacttatat
    18721 taggtggcta tctttctgag aattttgcac aggatttact attgtcacta aatgcatatg
    18781 tttgttttga acaaaattat ggtggaatag atggagatag tgcatcttct gcagaattat
    18841 atgtactatt atcatctctt agtggagtat caattaaaca aaatattgct gttacaggtt
    18901 ctataaatca aaagggtgat atacaagtag ttggtggtat aagtgaaaag atagaaggat
    18961 tttattctat atgtaaaact aaagggttaa atggtgagca aggcgtcata attcctagaa
    19021 aaaatcaaag aaatctcgtt ttatcagatg aagttaatga tgcagtgcga gatggaaaat
    19081 ttaagatata tactgttgaa agagtagaag aggctataga gatattgaca gatgttaaat
    19141 ttgaagaaat aaagaaattg gtaaaagaaa aattaatatc tttcagcaag atacaaactg
    19201 tgtcaaaaga ataagtttat tatagaattt aatgggtatt tatgtataat aaaaagcaag
    19261 aactttaata ggtttttgct ttttttgtaa atatatataa attaaattct tattttgtat
    19321 gttttatata ttgatgttta tttttttgtt gatatgatta aaccatttat aaatagcgtt
    19381 ttataaattg gtattttgaa gggggataat tatgaaaaat gtaaatctaa aagagataaa
    19441 tgaaaaagat gtaattttgg aagaagagat aaataaaaaa ggaatggata agaaaaatat
    19501 aattgagaag gaagagataa ataaaaaagg aat
//
LOCUS       DACXMZ010000058        19024 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.058, whole genome shotgun sequence.
ACCESSION   DACXMZ010000058 DACXMZ010000000
VERSION     DACXMZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.058"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            21..392
                     /locus_tag="JD016_07980"
     CDS             21..392
                     /locus_tag="JD016_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAU4999577.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNY"
     gene            503..1798
                     /locus_tag="JD016_07985"
     CDS             503..1798
                     /locus_tag="JD016_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAU4999578.1"
                     /translation="MLGGVIVVKKAIAALGIGAVAVSVSSINASALEKGTVTASALNI
                     RSGPSSDCDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSS
                     QNNSTSSGTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIK
                     LSNGVTGWASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSL
                     NVRSGPGTSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETSEGTKE
                     KPNSSSNQNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDC
                     SGFTQYVFKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYV
                     GNSKFIHCSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            1909..2853
                     /locus_tag="JD016_07990"
     CDS             1909..2853
                     /locus_tag="JD016_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999579.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            3069..4406
                     /gene="rsxC"
                     /locus_tag="JD016_07995"
     CDS             3069..4406
                     /gene="rsxC"
                     /locus_tag="JD016_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAU4999580.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            4429..5406
                     /locus_tag="JD016_08000"
     CDS             4429..5406
                     /locus_tag="JD016_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HAU4999581.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            5406..5975
                     /locus_tag="JD016_08005"
     CDS             5406..5975
                     /locus_tag="JD016_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HAU4999582.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDDLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            5988..6581
                     /locus_tag="JD016_08010"
     CDS             5988..6581
                     /locus_tag="JD016_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAU4999583.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            6596..7171
                     /gene="rsxA"
                     /locus_tag="JD016_08015"
     CDS             6596..7171
                     /gene="rsxA"
                     /locus_tag="JD016_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAU4999584.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            7181..8158
                     /locus_tag="JD016_08020"
     CDS             7181..8158
                     /locus_tag="JD016_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HAU4999585.1"
                     /translation="MKMVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGV
                     LPGANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVA
                     KVICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIV
                     DGIAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIG
                     CGMCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQEL
                     CVGCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            8378..8962
                     /locus_tag="JD016_08025"
     CDS             8378..8962
                     /locus_tag="JD016_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAU4999586.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            8959..9666
                     /locus_tag="JD016_08030"
     CDS             8959..9666
                     /locus_tag="JD016_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAU4999587.1"
                     /translation="MIYLKKSFNSTIKVKEMAPEERPREKMLVKGVKSLSNAELLAIL
                     LRTGNKNKNAIELANYIINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGS
                     RVASFKPIKYKIRNPWDIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSS
                     SLVHPREVFKEAIRRSASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDH
                     IIIGDGLYFSFKENMII"
     gene            9736..10797
                     /locus_tag="JD016_08035"
     CDS             9736..10797
                     /locus_tag="JD016_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAU4999588.1"
                     /translation="MAKEKKKEKKGFFSFNKMTKDMGIDLGTANTLVYIKGQGIVVRE
                     PSVVAIRDDSKEVLAVGEEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQK
                     AADKKGVVSPRIAICVPFGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVE
                     EPEGNMVVDIGGGTSEIAVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERT
                     AENVKINIGSTFKDDEEINMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIK
                     STLERTPPELASDIMENGIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTG
                     KSVEDQEIFEKVLMMNTKN"
     gene            10801..11700
                     /gene="mreC"
                     /locus_tag="JD016_08040"
     CDS             10801..11700
                     /gene="mreC"
                     /locus_tag="JD016_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAU4999589.1"
                     /translation="MVMALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSS
                     GSPVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNM
                     IALNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGV
                     KKESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDN
                     KDSYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYV
                     VVKPYVDFKNINDVVVIEPRNIG"
     gene            11715..12203
                     /gene="mreD"
                     /locus_tag="JD016_08045"
     CDS             11715..12203
                     /gene="mreD"
                     /locus_tag="JD016_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAU4999590.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            12215..15193
                     /locus_tag="JD016_08050"
     CDS             12215..15193
                     /locus_tag="JD016_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAU4999591.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMLTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEDV
                     ADLAVFSYFNFTKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVVRQGTAKSAFADFPIDVVAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            15291..15974
                     /locus_tag="JD016_08055"
     CDS             15291..15974
                     /locus_tag="JD016_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAU4999592.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYENESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            15998..16795
                     /gene="minD"
                     /locus_tag="JD016_08060"
     CDS             15998..16795
                     /gene="minD"
                     /locus_tag="JD016_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAU4999593.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            16813..17097
                     /gene="minE"
                     /locus_tag="JD016_08065"
     CDS             16813..17097
                     /gene="minE"
                     /locus_tag="JD016_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAU4999594.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            17182..18312
                     /gene="rodA"
                     /locus_tag="JD016_08070"
     CDS             17182..18312
                     /gene="rodA"
                     /locus_tag="JD016_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HAU4999595.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKIR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            18353..18766
                     /locus_tag="JD016_08075"
     CDS             18353..18766
                     /locus_tag="JD016_08075"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAU4999596.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
BASE COUNT         7297 a         2184 c         3826 g         5717 t
ORIGIN      
        1 aattggagag tgatttcaaa atgaagttta atttttgtca taacaatttc aatgttacta
       61 atttagaaaa aagtttagat ttttataaaa aagctttagg gcttaaagag gtaaagcgca
      121 aggaagcaga agatggaagt tttatattag tttatttagg agatggcatt actagtcata
      181 cattagaatt aacttggctt agagattggg atagaccata taatttagga gacaatgaat
      241 ttcatctggc tttagaagtt gaggagtttg atgaggcaaa aaaacttcat aaagatttag
      301 attgcatatg ttttgaaaat gaaagtatgg gaatttattt tatagcggac ccagataatt
      361 attggattga aatacttcct aaaaattact aattttaagg atatgtttat aatttcttta
      421 caattaataa gaaaataagt tatcattaaa ttgtagggac taaaaatgga taataatgtt
      481 atgaatttat gactataaaa atttgttggg gggagtgata gtagtgaaaa aagctatagc
      541 agctttagga attggggcag tagcagtatc tgttagctcc ataaatgcaa gtgctcttga
      601 aaagggaaca gtaacagcaa gtgccttgaa tataagaagt ggaccaagtt ctgattgcga
      661 taaggtagca aaattataca agggaaaaac tgtagagatt ttagaaaagt ctaatggatg
      721 gtataaagta agggtatcga gttctgttgt tggatgggga agtgcaaaat acatatcaac
      781 aagtggttcg tctgaaggta catcaagtca aaataattca acttcaagtg ggacaactat
      841 tagtggaaat ggtaaagtaa atgtgagttc tagactgaat gtaagaagtg gtgcaggtac
      901 taactattca ttagttggta aagcaaataa tggagatgta gtaaagttac tagaacaaag
      961 caatggatgg tataaaataa aactatcaaa tggagttact ggatgggcta gtagccaata
     1021 tatttctaaa acatcagaag atgttggaac gaacaattct agtaattcaa atagtactaa
     1081 taattctgat aaaaaaccat ctagcgaaga atcaattgaa ggcaagaatg gtaaggtaac
     1141 atctgctgtg agtttaaatg taagaagtgg tcctgggaca agctattcta taataggaaa
     1201 attaaatggt ggagatgttg ttgaattaaa agctaaaagt aatggttggt ataaggtaaa
     1261 gttatcgagt ggaacaattg gatgggtgag tgccagttat atttctgaga ctagtgaagg
     1321 tacaaaagaa aaaccaaatt catcatctaa ccaaaattct caatctaata gtaactcaaa
     1381 gccatcattt actggaaatt cagataaatc tacagcaaaa ggttcaacaa tcgtagattt
     1441 tgcatatact cttattggaa taccatatca atggggagct tcagggcctg ataaatttga
     1501 ctgttctgga tttacacagt atgtttttaa acattctgta ggcgtttcta ttcctagagt
     1561 ttctagagaa caggctaatt ttggaagtgc aatttctatg gggaattatg caccaggaga
     1621 tttagtttat tttgacactg atggtgatgg aacaactaat catgtaggta tatatgtagg
     1681 aaatagtaaa tttatccatt gtagtggtac tcagactaat cctaataagg ttaaagtgga
     1741 taatttaact agttcatatt ggtctaaagt attacttggt gcaagaagat ttgtttagta
     1801 agtacaattt cttattaaaa aattagaaga aataattatt tatattcata aattgtgact
     1861 cttaaaaata tactttatta ataatataat tttgggaggg acaaactaat gaatagaaga
     1921 aaaacgatat acagaggatt taataataat agacataaga ttgatttacg taaatatgtt
     1981 ataacgattg cttgcttgtg tctaattggc tattattctt atacaaaaat aaaagatagc
     2041 aagatattgg agtatgtatc ggctaaaata ccatttctaa acaattcatc agatataact
     2101 tataaagata tatctgatga attaaattca ataaaaaaag ggaaaaaatc taaatcacaa
     2161 accaattcag atgataaaca agaaactaat ccagaaaaag cggtaaataa cactaaagag
     2221 ccagaagaag tcaagttagc tactatagaa ggctgggata tgtatactat tcaagttgct
     2281 gcaatagata ataatgatga tttgaaaaaa atacagacat cattagttaa taatgacata
     2341 cctttttcag ttatggagaa ggatggagta aaaaaaatac aaacctattc ctcttttgat
     2401 gaaaatgatg ttagaaagca gataagcagt gtaagaaagg tgtttccaga tgcattttta
     2461 tcacacttag atgcacctat gttgtcttta gagtatacaa gtaactacgc ttatatagag
     2521 agtatatcta aagaattaaa taaattaata acaaacttta aagaagaatc aagtttttgg
     2581 tcgaatgctg aaaataatgt agatatggaa aaatataata ctattttgac aaatagaaag
     2641 gcaatatcac aaaatatatc aaaagaagct gaaaagattg actattctga aatgcgttta
     2701 tttaaagata atttaataga gtatgtaaag aatgtgaatg aaaaaattga tacagcttca
     2761 aaagcagcaa atgaagaaaa atatagtgta agtaaaagtc tgcttttatc atccatgcag
     2821 gagtactcta tgtttataaa ttctataaaa taaaatacag aaaatttgga ctttagaaag
     2881 ctatatgaaa aggattgttt aattaagcaa tccttttcct cggtttgact taatatagag
     2941 gtattgattt ataaaataaa ttataaaaat acggatattt ttttaacaat tgttaagatt
     3001 ttttaatata tatagtatac ttatagaagg cagaaaaaat gtttttttat agataagggg
     3061 gtaaaacaat gaaactctta acttttaagg gagggataca tcctccatat agaaaagagt
     3121 actctaacac aaaggcactt gaaaaagctc aagctccaaa gatagtttac attcctcttc
     3181 agcaacatat aggggcacca gctaaaccta ttgttgaagt tggtgatgaa gtaaagtttg
     3241 ggcaaaaaat aggtgaacaa caaggttttg tttcatgtaa cgttcattct tcagtatcag
     3301 gtaaagttat tgctattgaa caacatgaag taccaggagg ttcagctcaa tgtgtagtta
     3361 tcgaaaatga ctttaaggaa gaattacatg agagtgttca accaaaaggt cagttagagg
     3421 atttgagcaa agaagatata gtaggtatca taaaagaggc aggaatagta ggtatgggtg
     3481 gagctacatt ccctaaccat gtaaaagtat ctcctccacc agatagcaag gcagaagtag
     3541 ttatattaaa tggtgcagaa tgtgaaccat acttaactgc agaccataga ttaatggttg
     3601 aaaatccaga agatgttgtg tttggtttaa gagcattaat gaaggtatta gatgtcaaaa
     3661 aaggatttat aggaattgaa acgaataaac cagatgctat agaagcaatt caaaatgtag
     3721 ctaaagacta tagcgaaatc gaagttgttg gccttcaagt gaaatatcca caaggagcag
     3781 agaaacaact tatttatgcg tgtacaggca aagaagttcc atctggtgga ttaccaatag
     3841 cagcaggtgc agttgttgac aacgttgcca cagcagcaca gatagctaag tctattaaaa
     3901 caggtatgcc tttggttgaa agaatcacta caataacagg tagttgcatc aaagagccaa
     3961 agaacctaat aacaaaagtt ggaacattag tttcagaaat aatagaacaa tgtggtggat
     4021 ttaaagaaga taagaagatt ggtaaggtta taatgggtgg acctatgatg ggaatagctc
     4081 aatatactac tgaaatagct actaacaaag gttcttcagg aattttatgt ttggatgagg
     4141 aagaatcacg tactccagat attcaaaact gtttaagatg tggaagatgt acagatgtat
     4201 gtccatcatt cttacaacca cttttcataa gtgcatattc tttaaaagat gactatgaca
     4261 cagctgaata tcatagagca atggattgta tagaatgtgg gtcttgctca tttatttgtc
     4321 cagcaagaag accattactt caatctataa gatcagcaaa gagagaaata ggagcaaaga
     4381 gaagaaagca agctgctcag aaataaagaa agttaagggg gaaaaacgat ggaaaataag
     4441 ttgatagtat catcttctcc tcatgtgaga agtaatgaag atacttcata tataatgaaa
     4501 caagttatta tagcactcct tccagcagca gtagcaggag tatacttctt tagacttaat
     4561 gctttgagtg ctatgttttt ttgtatactt ggtacagtag gtactgaatt tttatatcaa
     4621 aaatttacga agcaaaaaag tactatagga gatttttcag ctgttgtaac aggattatta
     4681 ttagcattta acgtaccagc atcacttcct tggtggatgt gtctagtagg aggaatattt
     4741 gcaatattag tagttaaaat ggtatttggt ggaattggat gtaactttgt caatccagca
     4801 cttgctgcaa gagcattttt attagcatca ttcccagtag caatgactgc ttggactcaa
     4861 ccaggtgtta actggatagg taaaaattta gatgcagtta ctacagcaac tccattaagc
     4921 tttttgaaaa atggagcagc aggactggca gacctttcta gcaatggaat tagtcttgct
     4981 gacatgatga tagggaatat tggtggatgt ataggtgaga catcagcaat attattatta
     5041 ttaggtggag tatacctaat gtataaaggc ataataaatt atgttatacc agtattttac
     5101 atagcaactg tatttatatt aacattcctt ttaggtggat ttaatataac tttcgcaata
     5161 tatcaactgt ttgctggagg acttatgtta ggtggattct tcatgcttac ggattataca
     5221 acttcgccta tgactaaaaa aggtcaaata atatacgctg tattagctgg tcttataaca
     5281 actgttataa gaatgtatgg tggatatcca gaaggtgtat cttactcaat actattagtg
     5341 aactgtcttg ctccacttat agataagttt gttagaaata gagtatttgg ggaggtggct
     5401 aaataatgaa cagtatggtg agattaggtg gtactttact tgctataagt gcaatagctg
     5461 cattagctct tggagctact aatcaggtta cagctccagt aatagaacaa agaaatatac
     5521 aagcaaataa tgaacttaga aaagcagtcc ttccagaagc aaaagaattt aaagaaatga
     5581 aggaagatac ttacaaaggc cttggagatg atcttatagc tgaagtgtat gaaggtttag
     5641 atggttctga agtggttgga tatactttaa aagctaaacc aagtggatat ggtggagaaa
     5701 ttgaagttat ggtaggtata tcatctgaag gtcaagttac aggtgttgac attggtaata
     5761 tgtcagaaac agcaggtctt ggagcaaaag ctaaggatga tgcatttaag ggtcaataca
     5821 aaggaaaaac tgctgagcct cttgaagtag caaaaggaag tacaacagca gataatcaaa
     5881 tattagcaat atcaggtgca actattacat ctacagcagt tactacagga gtaaatgctg
     5941 ctatagatgt tttcaatagt gctcttaata aataaggagg gttaattatg aatttagcta
     6001 aagtatttaa aaatgggcta atagatgaga acccaacatt tgttcaagtt ataggtatgt
     6061 gtccaacact agccgttaca acttcagcaa taaatggaat tggtatgggt ctttcaacag
     6121 cagcagtttt aatatgtgca aatttagtta tatctttaat aagaaaaatt acaccagata
     6181 agataagaat acctatattt atagtagtta tagctacatt tgttacaata gtaggtatgc
     6241 ttttaaaagc atatgttcct gctcttgata aggctcttgg tatatatata ccattaatag
     6301 ttgttaactg tttaatatta gctcgtgcag aaagttttgc tttcaaaaca ggagctatgc
     6361 cttcaatagt tgatggtgta ggtcaaggtc ttggatttac tgttgcactt acaataatag
     6421 gtgcagtaag agaattactt ggaaatggta gtttatttgg aatgacatta tttggagcat
     6481 ctttccaacc agtattaata tttatattac caccaggtgc attcttaaca ttaggattct
     6541 tatttgcagg atttaataaa ttaagaagta aaaaagcata gtggaggtgt aataaatgaa
     6601 tttaatactt ttatttttaa gtatcgttct tgttaataac gttataacat ctcaattctt
     6661 gggtatatgt cctttcttgg gagtttctaa gaaggttgat acagcagtag ggatgggagt
     6721 tgcagttacg ttcgttttaa ctctagcttc aattataact tattttatac agattttatt
     6781 agtaaaaact ggaactggat tcttacaaac aatagctttt atattggtta tagcttctat
     6841 agttcagttc gttgaaatgg taattcaaaa gatgagtcca tctttatatc aagcattagg
     6901 agtttattta cctcttataa ctacaaactg tgcagttctt ggtatagctc tagttaatgt
     6961 tcaaaaaagt tataatttag tagaaacaat aataaacgga tttggagccg gagctggatt
     7021 tactttagca atagtaatat tcgcaggtat aagagaaaga ctagaattag ctgatatacc
     7081 agaagcattt aaaggtttcc ctataacgct tatatcagca ggtttaatgt caatagcttt
     7141 cttaggattt acaggactta taaagctata aaaaagaggg gtgaaaatgg tgatacttac
     7201 agctgtatta gttttaggaa ttatgggctt aatcttcggg atagtactag attttgcatc
     7261 taaaaagttt gctgtagaag tagacgagag agtggaagct atactaggag tacttccagg
     7321 agccaactgt ggaggttgtg gatttccagg ttgtggaggt cttgcaaatg cgatagttga
     7381 aggcaatgca ccagttaatg gttgccctgt aggtggagca gatgttggtg caaaagttgg
     7441 agaaataatg ggaataagtg ctgaagcagg tgagaaacaa gtagcaaaag ttatttgtaa
     7501 aggaacttgt tcaagtgcaa aagataaata tgagtatgaa ggaatatcag attgtagagc
     7561 agcaaatgtt ttaaactcag gtgctaagat gtgtaaattt ggatgtttag gtcttggaac
     7621 atgtaaagat gcttgtaaat ttgatgctat ttctatagtt gatggaatag cagtaataga
     7681 tgaagaaaag tgtgtaaatt gtggaaaatg taaagaagtt tgtcctaagg gtataataat
     7741 cacaaaacca gaaagtcaag aagtagttgt agagtgtaat agcaaagagt ttggtaaagc
     7801 tgttaaggaa aaatgtactg ctggatgtat tggctgtgga atgtgtgtaa aagcttgtaa
     7861 atttgatgca ataatatttg aagacaaaat agctaagata gaccctaata agtgtgtagg
     7921 ttgtatgcaa tgtgtagcaa aatgccctac aaaagtaata tcaggtgaca taactaagaa
     7981 aaagaaagtt actattgacc aagaactttg tgttggatgt acagtgtgca aaaaacaatg
     8041 taagtttgat gcaatagagg gagaattaaa agaaaaacat aaggtagatg cagataaatg
     8101 tgtaggatgc catttatgta tggaaaaatg tccaaagaaa gctattaaaa ttttgtaata
     8161 tggacaaaat aaggatatgg aataatttcc atatctctaa tgagtacata aaacagaggt
     8221 atggaataat ttccatacct ctattttatg tatatttatt ctaatggtaa taaatataac
     8281 aatagtgtaa caaaaaaggt tcgacatttc tgttgaattt tttacaatat agtgataaac
     8341 taaataggta caaattacgt acaaatgagg tgaataaatg aatataatct tggcatcagc
     8401 ctctccaaga aggaaagaaa ttctagagaa tacgaatgta agatttgata taattaaaaa
     8461 tgaaattgat gagattatat tggaaggtga aacaccaaaa catttagtta tgagattagc
     8521 ttttgaaaaa agtatgtctg tagcatctga acataatgaa gatgttgtta ttggagctga
     8581 tacagttgtt gttttagaca atgctatttt aggaaaacca aaagatgagt cttgtgcaag
     8641 agatatgtta aaaagactat ctggaagaga gcatcaagtc ataacaggta ttagcttaat
     8701 aaacttgtgc gaagataaaa aggtaattga ctatgtaatc agcaatgtga aatttaaaac
     8761 gttatcagag caggatattg aggattatct gaaaactaat gagtcatttg acaaagcagg
     8821 agcctatggt atacaaggtt atggagcttt gttagttgaa gaaattagag gagattattt
     8881 taatatagtt ggtcttccaa tttcaaggtt aggcgaccta ttaaaaaaat attttagtat
     8941 aaatcttttt tatggagtgt gatttatctg aaaaagtcat ttaattcaac cattaaggta
     9001 aaagaaatgg caccagagga aagacccaga gaaaagatgc ttgtcaaagg cgtaaaaagc
     9061 ttatcaaatg ctgagttgtt ggctatactt ttaagaactg gcaacaagaa taaaaatgct
     9121 attgaattag ccaactatat aataaataga gatattcagg gtattagaca tttagaagat
     9181 atgacaatag aggagctgtg taatatagat ggaattgggt tatcaaaatc aacccaaatt
     9241 aaagcagctt tagaactagg ctctagagta gctagtttta aacctataaa gtataaaata
     9301 aggaatcctt gggatataca gaggtattat atggatagcc taaggtattt aaaaaaagag
     9361 gtttttaaag cggttctttt aaacacaaaa aatgagataa tatctgatgt agatgtatct
     9421 atagggactt taagctcgtc attagtccat ccaagagagg tttttaaaga agctataaga
     9481 agaagtgcaa gtaagataat agtgatgcat aatcaccctt caggaagtgt agaaccatca
     9541 agagaagata aaaatatcac atcaagactt attaaatgtg gagaaataat tggaatagaa
     9601 ataatagacc atataattat tggagatgga ttatacttta gctttaaaga aaatatgata
     9661 atttgacttt atatgtgata aaataatagg agaatgtttt aaacatgatt cttaatagta
     9721 ggaaggagta aaatcatggc taaggaaaaa aagaaagaaa aaaaaggttt ttttagtttt
     9781 aataagatga caaaggatat gggaatagat ttagggactg caaatacatt agtttacata
     9841 aaaggacaag gtattgttgt tagagaacca tcagttgtcg caataagaga tgatagtaaa
     9901 gaggtattag cagtaggtga agaagccaaa aaaatgatag gtagaacacc aggaaatata
     9961 gtagctataa gacctatgaa agatggagta atagctgatt ttgacataac tcaatcaatg
    10021 attagctatt ttatacaaaa agcggctgat aaaaaaggtg ttgtaagtcc aagaatagct
    10081 atatgtgttc cttttggagt tacagaagta gaaaagagag ctatagaaga ggctgcaaga
    10141 caagcaggag ctaaagatgc attccttata gaagagccaa tggcagcagc tataggggct
    10201 ggattaaaag ttgaagaacc agaaggtaat atggtagttg atattggtgg aggtacttca
    10261 gaaatagctg taatatcttt aggaggaatc gttactgcta aatctataag aataggtgga
    10321 gacgaatttg acgagtctat agtggcttat gtaaaaaaag aatataatct tatgataggt
    10381 gaaagaactg ctgaaaatgt aaaaattaac ataggttcta catttaaaga tgatgaagaa
    10441 ataaatatgc aaataagagg tagagattta atctcaggat tacctaaaac aatagaaata
    10501 tgctctacag aagttagaga agcattaaaa gaacctgtaa gttcaatagt agatgcaata
    10561 aaatctacac tagaaagaac tccaccagaa ttagcctcag atataatgga aaacggaata
    10621 atgctaacag gaggaggcgc tttgcttaga ggtcttgata agcttattac acaggaaact
    10681 ggtatgtctg tacaaatagc agaaacacct cttgattgtg ttgcacttgg aacaggtaaa
    10741 tctgttgaag accaagaaat ctttgaaaaa gtattaatga tgaatactaa gaattagaag
    10801 ttggtgatgg ccttgagatt tgacaagaac aaaaatgaga aaaaaaggat taatgttaaa
    10861 gtgatagcaa cgggcgtagt tgctatcact ttaattggaa ttgtgggtat atcaattggt
    10921 aaattttcta gtggtagtcc agtaaattta ggagtagctt cagatgctat tacaagtgta
    10981 ggtaagggaa taaatgatgg attttctttt atcaaaaatg gttttaaaga tgtagcaaat
    11041 tttaaagata attctaaaaa ggtaaaaaaa ttagaagaag aaaatgaaaa acttaaaaaa
    11101 aatatgatag ctttaaatgc caaactagat aaaacagaat cgctagaaga gttaaaaaag
    11161 acactgaact ttgtacaaga agagtataag gcgacaagta tttcagcaag tgttgttggt
    11221 aaaaatgatg gaaactggta tgagagtttt gtcataggtg caggaaaaaa ttcaggtgta
    11281 aaaaaagaaa gtatagttat gaatggtagt ggattagttg gcatagttta tgaagtttcc
    11341 aacaattata gtaaggcaat ttcactattg gattctaagg catctgtaag ctttaaacta
    11401 gcaaaggatg ccaatgctaa aggtacaatt actcaaaata ctactttaga taataaagat
    11461 agttataata gtaaaggata tttgcaagga tatatgtttg actcatcata taatgtaatt
    11521 caaggagata taataacaac atcaggtctt ggattcttcc cagatggtat accaattggc
    11581 gaagttgaaa aagtggttga tgataaagat aagtcattaa aatatgtagt tgtaaaacct
    11641 tatgttgatt ttaagaatat aaatgacgtt gtagtcatag aaccaagaaa tataggataa
    11701 aggggataaa aacaatgaaa aaagttttac tttgtctgtt ggggattgtg cttatgacta
    11761 tagaaaatag tataacaaat tatatagata tttttggcat aagctttaat ttagttctta
    11821 tatatgtaac cataatctct ctttatttag atgaattaga aggtgggatt atagcagcaa
    11881 taatagggct tattaaagac ataaccgttg gtggaatttt tggtgtaaat gcattaattt
    11941 tgtttattat tgcatatgca attggataca tgagagatca gctatataaa ggtagttata
    12001 ttattacttt cgttttaatt tttataggca gtttaattga ttcaattgtc aatataggta
    12061 catctagtat aatttatcaa agctacaaca tatcaacact ttttgtaaaa ggtctactag
    12121 tagcaccaat agtgaatagt ttaattggtt tagtaatata tcgcttatct aaaagagcag
    12181 ttttaaaact taaagaagat tagttggtga tttcatggaa gaaaataaac aagttgatag
    12241 actaaaaata attaagatag tcatgggcgt gatattttta gctattttag taaaaataat
    12301 ctatatgact acatttaaat atgaatatta caatgagtta gctgagaata aaacatataa
    12361 aaagttggca atagaagctc caagaggaga aataaaagat agatatggaa gactgttggc
    12421 aggaaataaa aacttattta ctgttcaagt ttcaggtaac gatattaata agaaagatgc
    12481 aaataagcat tcaagagcaa atgaaatatc attaaaactt ataaatttgc tggagagaaa
    12541 tggtgaagaa tatgtagatg agtttcctat atatgtagaa aatggaaagt actattatac
    12601 atatgacaga gatattaggg aatataaatc ggaaaatggt attccaaatg attataatgc
    12661 taaggaaagt ttttattact tagtagacaa acttatatca gctggaatat tatctcaaga
    12721 agataaaaga ttggatgcga caagattaca ggcaaaacta aatgaaaatg gatattatcc
    12781 acctatttta gttagtaaat ggatgttcac agcagaaaga gataagagag attggttggc
    12841 tagttataag ataaaagaaa ctaaactgag tgccaaagaa gcgtttgaaa aagtaagaaa
    12901 tagtgatgca ttagaaattg ataaaagttt gagtgatgaa gatgcaagaa aaataatggt
    12961 cgtaagggat ttgataaaat caaaaggata ttctcaatat aatccagtaa ctattgctaa
    13021 ggatgtagga gaaactacga ttgctcaaat tgaagaaagt gctatggacc ttgttggtgt
    13081 ttcaatagca gtagaaccag taagatatta tcctaatggg tcattagctt ctcatatgtt
    13141 aggatatgta ggaaaaatgc catccactca aatagaatca taccttcaaa aaggttatga
    13201 aactggagat atggtaggtc ttgcaggagt agaaaagagt aatgaaagta gattaagagg
    13261 aactgatggt tataaaatgg ttaaagttga tgcattgggt agaatcagta aagaaattga
    13321 atctaaaaaa cctaaatcag gagatactgt gtatcttaca ctagataaag atttacaaga
    13381 agtttcagac aatgcattaa aacagataat cgaagttgct tctaaaggag gtacttttaa
    13441 aagtaaattt ggggacaaac caattagtgc atatgcagga aaagcacagt cagcagctct
    13501 tatagctatt gatgttaaaa atggagaagt attagcttca tcaagttatc ctaattatga
    13561 cccaaataaa tttgcaaaag gtatttctac agaggattat aaagcacttc aaccaaaaaa
    13621 tccaaatgac ttactagcag gaagtccact tttaaacttg gttacacaag gggaatttca
    13681 gccaggttct tcatttaaga tgctaacttc aatggcagca ttagaaaatg gtctggatcc
    13741 aaactttact ataaatgacc ctggggttat aatgttaggt aaaaaatctt ttggtgacta
    13801 tgtatggaac catggtagag gaaaccatgg tatgacaaac ttatataaag caatacaaga
    13861 gtcttgtaat atatatatgg caacaattgg tacaggtaaa acatggccag atggaaaaag
    13921 cattggtata gatatgaatg caaataaaat acttgagtat gcaaaattat ttgggcttga
    13981 ccaaaataca gggcttcaag atgaagtaga agaaagagca ggcaaagttc ctagcacaga
    14041 agataaattg aaatcaacac aagctcttct aaagtcaaat ttagaaagag aaatggcaaa
    14101 tgattttgtt gatataacta gagaaaaaaa tcctaaagaa tatgagaaaa gaatcaatga
    14161 gattgtaagc tgggcagcag aaaagaaaac tcctggtaga gtcgaaacaa tgaataggct
    14221 aaagaaaatg aatgttaaag aagatagaat agaagatgta gcagatttag cagtattcag
    14281 ttattttaat tttacaaaat ggagtactgc agatacattc aacttagcta taggtcaagg
    14341 tgaaaatgct tatacaccag ctcaaatatc aagatatgta gctgccatag caaatggagg
    14401 taatttagta gaattatctg tagttgatag agctgtttca agtgattatt catcagttaa
    14461 aattaatgac cagaagaaag tagaaaaaat accttttaaa aatccagata acttaaaaga
    14521 attaacaaaa ggtatgaaat tggttgtaag acaaggtaca gcgaagagtg cttttgctga
    14581 ttttccaata gatgtagtag caaaaactgg tacagctgag aaaagtggga aaatacctac
    14641 agataacgag tatgaatatt taaaaagtca tatgtcatct tataatgtca acctaaatga
    14701 tgcgattaaa ctagctgata agatgaaagc agaaaaagaa aaagagcttt cattagctaa
    14761 ggaaaaggaa ataaaaaaga aattagaaaa taaagatttg aaagacgaag aaagaaagaa
    14821 attagaggaa gaattagaag atggagtaaa agttagatta gaggacactg ataaggtgaa
    14881 ttcttcatat ctaagaaaag ctataaaaga attaaatcca aaaataacag atgaccaaat
    14941 agatagattt aaacaagatt atggttcttt tacgtggact gttgcttttg ctccagcaga
    15001 tgacccagaa atagctgttg tatgtgtaat tcctcaaggt gattcaagtg tattctcact
    15061 tttaccaact agagaagtta ttggaactta tatgggatta gagccaacta atagtaaaaa
    15121 tgacaacaag acgaatgatg ttaacaattc ttctgacgaa aatataaatt tcgagtcaca
    15181 aattaataga tagctcaatc tatgtataaa ctttagcttt atatagaaaa aataaaaggg
    15241 tttaaagcgt atttgaagaa tataaatgat atatcactct ggaggttgtt atgtctttaa
    15301 gagaaatttg tagtcaagaa ctagtagagt tcaagggtaa taaaagaggg ataatagtaa
    15361 atataaaaag agaagctcca tttgaggaaa tacaggaaaa aataataaac aaattggaag
    15421 catatgttgg cttttttaat ggggctaaaa ttagtaaaat taatagtgat tgtttaactg
    15481 acatggagat tctagaactt aaagaaggaa ttacttctag atttgatgtg gaatttgttg
    15541 aagatcaaaa aattgaagaa aatagtaatt ttccaacaaa atatgtcaat accctaagat
    15601 ctggtgaaaa tattgaattt gaaggtgatg tagttatatt gaatgatatg aaacctggtt
    15661 caaaagtcct ttcaaaatca aatacagttg ttatgggaga tataaatgct ggtgctaagg
    15721 ttgtagcagg aggtaatgtt tttgtaatgg gtaaaataga aggttttgta catgcaggtg
    15781 cagaaggcaa tgaatttgca tatgttgttg ctggaaatct taatcctaaa atattacaaa
    15841 tagcagataa tattgcagaa gctccagatg atgaagaaaa ttatgaaaat gaatcagaaa
    15901 ttagcccaga aatagccttt gtaagtaatg gaagaattgt aatcgaaagc tatttgtcaa
    15961 agttagacaa atagaaagaa aaataggggg tagtattatg agcgaagtta tagttataac
    16021 atctggtaaa ggtggggttg gaaaaactac taccgcagca aacttaggaa ctgcgctaag
    16081 cctagaaaat aaaaaaacag tagttgtgga tgcagatatt ggacttagaa acttagatgt
    16141 agtaatgggt cttgaaaatc gaatagttta tgacatagta gatgtagttg aaggaacttg
    16201 taggcttaag caagctctaa taaaagacaa aagatttgat aatctatatt tattaccagc
    16261 agcacaaact agagataaaa atgctgtctc agttgagcag atgattgacc tgtgtgagaa
    16321 actaaaagaa tctttcgagt atataataat agactgtcca gcaggtattg agcaaggctt
    16381 taaaaatgca gtagctgggg cagatagagc tattgtagtt actaatccag aaatatcagc
    16441 agtaagagat gctgatagaa taataggtct attagaagca aatgaaataa aagagataag
    16501 attagttata aatagaatta gaaatgatat ggttaagcgt ggagacatga tggataaaca
    16561 agatataata gaaatattag caatagattt gttaggtctt gttcctgatg atgaaagcat
    16621 aattatatca acaaataaag gagagccagc tatacttgat tctaagtcac ttgctggtca
    16681 agcatacaaa aatatcgcaa aaaggatact aaatgaagaa gttcctctac ttgatcttga
    16741 agttgaagat ggattctttg gtagacttaa aaaaatgttt agcatggcta agtagcaaag
    16801 gaggatacat ctgtgttaga tttatttaga gttttttcta acgaggctaa aactagtaaa
    16861 tctgttgcta aagagaggtt aaagctagtt ttagttcatg atagagtaga ttgttcacca
    16921 caacttttag agatgataaa aacagacatt ttaaaagtaa tagctaatta tgcagaaata
    16981 gaagatgatg gtcttgaaat aaaaatgtct aaatgcagag gtgagcatga tgacaaacct
    17041 gtatctgcat tagttgctaa tataccacta aagaatataa aagatagatg tatgtaatta
    17101 gtgcttaaaa gggggatatc cttattggta tatgcccctt ttatttaatt atatgtttaa
    17161 agatagaaag acggtgattc tttgaaaaaa atcaatatta attataagtc gacaattaag
    17221 ttgataaaac agttagactg gaaacttata gttacagtat tagctatctt tatttttggg
    17281 cttgttatac ttagtagtgc aacacatgct aattcaacag gttcatataa tcaattgatt
    17341 aaacaaggat tagcttttgt acttggtata ggaatgatta tagtcatatt attttttgac
    17401 tataaccttc taggcaggta ttataaagcg ctttatataa taagtcttat tttgttagcc
    17461 atagttttat tacctggaat tgggacagta aaaggtggag ctaggtcgtg gattaactta
    17521 gggccacttg acttgcagac atctgaaata gtcaaattaa catttgtact aagttatgca
    17581 aagatacttg aatccaaaaa ggataaatta aatacattaa aggaagttat gcctgttgta
    17641 gtttattctc ttccatttat cggattattg attgcacagc cagacttggg aacaggaatt
    17701 gtattttgtt gtatgatatt tgcaatgctg ttcacagcag gtcttagctc aaagttgata
    17761 aaaagaggaa taatcatact attggtgtca atgccactga tgtatttaat gatggcagac
    17821 catcaaaaga taagaataga agcttttcta aatccagaag atgtaacact aaaaggaaac
    17881 tatcaagtta tgcaatctct aattgcaatt ggttctggag gtgtaactgg taaaggactt
    17941 tacaatggaa gtcaaaatca agaagatttt ttacctgtac aagatagtga ttttatattt
    18001 gctgttgtag gtgaagaact tggtgtaata ggaatggcag tgttgataat cttatttatg
    18061 atattcctat tgaggctttt agcaattgca agagatgcaa aagattttta tggaaccctc
    18121 atcgtggttg gtgttatggg aatgtttggt taccagataa tccaaaatat aggtatgaca
    18181 gttgcgttaa taccagtaac tggagtaaca ttaccttttg taagttatgg aggtagttcg
    18241 ttattgacat ctttagcaaa cttaggatta gtactaaatg tgtgtatgag aagaaagaaa
    18301 ataaatttct aaatttaaat acatctaaac ataaattgag gagattagat atatgaatat
    18361 agcattagta gcacatgacc aaatgaaaaa cactatggtc ggattttgta taggttatga
    18421 atcaatttta aaaaagtatg gattatatgc aactggaact acaggaaaaa gaataatgga
    18481 tgaaactgaa ttaaatataa atagattagc atctggtcct ttaggtggag accaacaaat
    18541 aggttcttta atagtaacac aagaaataga tttagttatt tttttaagag acccattgac
    18601 atctcaggct catgaaacag atatacaagc gttaataaga ctttgtgatg tttatcatgt
    18661 tccaatagct acaaatctag cttcagcaga aatatttata aaagctcttg atagaggaga
    18721 actatcatgg agagaagtta gaaaaagcaa aagtcaacgt atttaaaaaa tactaatatt
    18781 acagtctaaa gaaacagata tataagtgtt taatgcccta gtctttctgt tgcaataaac
    18841 ctagtacctg tataaatctt atgaaagatt taaatgatga agagctaaat ttgagattaa
    18901 ataattagga aatagattat agatagagta gtagaattta aactattact ctattttaat
    18961 aatatgaaat agaatcaagt ctattattat atatatttaa acttataggt aatactattt
    19021 caat
//
LOCUS       DACXMZ010000059        18948 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.059, whole genome shotgun sequence.
ACCESSION   DACXMZ010000059 DACXMZ010000000
VERSION     DACXMZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.059"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(77..2071)
                     /locus_tag="JD016_08080"
     CDS             complement(77..2071)
                     /locus_tag="JD016_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HAU4999597.1"
                     /translation="MYERRASILNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLK
                     FSEVYRELTDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKA
                     GKLTFGGEAKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVH
                     KDDEKTRVTFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKT
                     YLSRAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVG
                     RKRGAGLGGGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDR
                     QVVVGTPDVKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTA
                     RKREKKIKMETIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPV
                     HQVTIVPRGRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASN
                     DLERVTATARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETK
                     RIVDGAYDKTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTE
                     ENSSKEVEEQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2081..3469)
                     /gene="tilS"
                     /locus_tag="JD016_08085"
     CDS             complement(2081..3469)
                     /gene="tilS"
                     /locus_tag="JD016_08085"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAU4999598.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3652..4065)
                     /locus_tag="JD016_08090"
     CDS             complement(3652..4065)
                     /locus_tag="JD016_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAU4999599.1"
                     /translation="MSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVIYKEKE
                     EDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDTRELDI
                     NIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4097..4903)
                     /locus_tag="JD016_08095"
     CDS             complement(4097..4903)
                     /locus_tag="JD016_08095"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAU4999600.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5105..5611)
                     /locus_tag="JD016_08100"
     CDS             complement(5105..5611)
                     /locus_tag="JD016_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HAU4999601.1"
                     /translation="MDRWSKLMATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIA
                     KKEVINLSDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQK
                     NAFSVVLDGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMR
                     IGNHVFAK"
     gene            complement(5641..6300)
                     /gene="spoIIR"
                     /locus_tag="JD016_08105"
     CDS             complement(5641..6300)
                     /gene="spoIIR"
                     /locus_tag="JD016_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HAU4999602.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6445..7101)
                     /locus_tag="JD016_08110"
     CDS             complement(6445..7101)
                     /locus_tag="JD016_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU4999603.1"
                     /translation="MDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLAEQLGVSRT
                     PVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYLAAERISDE
                     DIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINSLWEQVYRF
                     RVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMIDKAMISKDL
                     "
     gene            complement(7109..7999)
                     /locus_tag="JD016_08115"
     CDS             complement(7109..7999)
                     /locus_tag="JD016_08115"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAU4999604.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8117..9667)
                     /locus_tag="JD016_08120"
     CDS             complement(8117..9667)
                     /locus_tag="JD016_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HAU4999605.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9761..10027)
                     /locus_tag="JD016_08125"
     CDS             complement(9761..10027)
                     /locus_tag="JD016_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999606.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10175..11035)
                     /gene="yabG"
                     /locus_tag="JD016_08130"
     CDS             complement(10175..11035)
                     /gene="yabG"
                     /locus_tag="JD016_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HAU4999607.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11119..12057)
                     /locus_tag="JD016_08135"
     CDS             complement(11119..12057)
                     /locus_tag="JD016_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HAU4999608.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12167..12328)
                     /locus_tag="JD016_08140"
     CDS             complement(12167..12328)
                     /locus_tag="JD016_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999609.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12338..12835)
                     /locus_tag="JD016_08145"
     CDS             complement(12338..12835)
                     /locus_tag="JD016_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HAU4999610.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(12950..13660)
                     /locus_tag="JD016_08150"
     CDS             complement(12950..13660)
                     /locus_tag="JD016_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999611.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYALGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFGYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13836..14366)
                     /locus_tag="JD016_08155"
     CDS             complement(13836..14366)
                     /locus_tag="JD016_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999612.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14383..14613)
                     /locus_tag="JD016_08160"
                     /pseudo
     CDS             complement(14383..14613)
                     /locus_tag="JD016_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090098.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..16022)
                     /locus_tag="JD016_08165"
     CDS             complement(14628..16022)
                     /locus_tag="JD016_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HAU4999613.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16148..17401)
                     /locus_tag="JD016_08170"
     CDS             complement(16148..17401)
                     /locus_tag="JD016_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAU4999614.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17452..18606)
                     /locus_tag="JD016_08175"
     CDS             complement(17452..18606)
                     /locus_tag="JD016_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAU4999615.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVVGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIKKLK"
BASE COUNT         5853 a         3611 c         1863 g         7621 t
ORIGIN      
        1 aatttaatat tatactttca gttttagtat ttaatccaca tcagataata atcttaaaat
       61 gaccaaggtc ttaaaactat ttatctttat cagatttatc ttctaaattt ttatttatat
      121 caataacatt gtttctttct tcttcatctt tttctaattt tatagttaat tgttcttcaa
      181 cttctttaga agaattttcc tctgtaactg cattttcaat ttcattcaga ggtaactctt
      241 tattaaatgc ttttacaaat tgttctgcat ctaaagtttc atatataagt aaagcttgtg
      301 ctacatactc taatctatcc atattttctt gtaatatact tcttgtctta tcatatgcac
      361 catcaactat acgctttgtt tcttggtcta tctcaaaagc aacttcttca gaataatttc
      421 tcttacttga aaaactatta cctaggaata cttcatcatc actgtcaaat gacattggac
      481 caagtttaga actcattcca tactttgtta ccatacttct tgcagtagct gtaactcttt
      541 ctaagtcatt tgaagcacct gtagatacat ccttaagtac tagctcttct gctactctac
      601 caccaagtaa aactacaata ttttctttca tttcattttt tgtagcatag aacttatctt
      661 caacaggaag ttgcattgta aaccctccag ctcttcctct tggaactatt gtaacttgat
      721 gaacaggact tacttcttca agtacatggg cacaaactgc atgccctcct tcatggtatg
      781 ctgtaagtct tctttctttc tcacttatta ctttcgattt tttagccaca cctgctataa
      841 cctttgttat agcttcttct atagtttcca tcttaatttt cttttctctt tttctagctg
      901 ttaatattgc agcctcattc attaagttct ctatatcagc aggagtaaat cctggtgttc
      961 ttctagccaa gacatccatt tttacatcat cacttaatgg tttatttctt gagtgaactt
     1021 taaatatagc ctctctacct tttacatctg gtgtaccaac aacaacttgt ctgtcaaatc
     1081 ttcctggtct aagtaaagct gggtcaagta tatctggtct atttgtagct gccatgatta
     1141 ttataccttg atttacccca aatccatcca tttcaactag aagttgatta agagtttgct
     1201 ctctttcatc atgacctcca ccaagacctg cacctctttt tctaccaact gcatcaattt
     1261 catctatgaa tatgatagct ggagcacttt tcttagcctg ttcaaataaa tctcttactc
     1321 ttgaagcacc aaccccaaca aacatctcaa cgaaatcaga accacttata ctgaaaaagg
     1381 gaactcctgc ttctcctgca actgctcttg atagatatgt tttaccagtt ccaggaggtc
     1441 ctaccataag cattcctttt ggtattcttg cacccaattc aatatatttc ttaggattct
     1501 ttaagaaatc tactacctcc tgcaaatctt ctttttcctc atctagtcca gctacatctt
     1561 taaatgtaac tctagttttt tcatcatctt tatgaacctt agcttttgat ttgccaaaac
     1621 tcataacctt tccgcctcca ccttgagact gattcataaa cacgaaccaa ataatcacca
     1681 tgaagaatat taacagtaat gtaggcaaca tttcgacaaa ccaaggtgta gatggcttag
     1741 cttcaccacc aaaagttagt ttcccagcct tagcttggtc taacacctca tctgcaagtt
     1801 tatttcccat cacttctgtt ggaacatatg acttaaactt tgtatttgta tcttttatag
     1861 taccttctac agaagtttga tttacaaagt ataatcttga tatattctca tctgttaatt
     1921 ctctatatac ttcagagaat tttaaatctt ttattttttc tgtaggctta cctgagaatt
     1981 gaactatacc tactataatt ataaaaacaa gtagataaaa acctgcccct tttagcaact
     2041 tattcaatat agaagccctc ctttcataca ttgctttgcc ctataatttg ttaaaattaa
     2101 tttttaatac ttcttttgtg ttttcatcaa ttttataatt ttcactgatt ttataatctc
     2161 caacacatat tattccttca ctatctgtga ttataggaat tttacatctt tcttctcttg
     2221 ggatttttaa atctatgaat aaatctttca cttttttact tcccatagca agctttatct
     2281 tatcaccttg ccttctgctt cttattacta tacccccttt taccttatta aaatcaaacc
     2341 ctttagatga attgtctaat ttcatactct tatacctatc tatgctcatt acttgggtct
     2401 caataatttg ttttaattct tttattttaa taaacccatt actaggtaca ttgtaataaa
     2461 attcaatttc ctcattaaca atttcctcat tcgttaatat tatactgtct ttttttctat
     2521 aaacaaatat atttcttgga agagttaaca ttttatttac ttttgaatta tcttctaaag
     2581 acattatatc ctctatatgt ctttggtcaa caaaattagt atctcctagt atatgtttta
     2641 tggaatttct aagaactcta actttgatag catcatgtaa gcaaacaaaa tcgtctaaat
     2701 ttatctctac aaagcctttt tcttttatat ttgaaacttc tctaaattta gcttctgcct
     2761 ctttctcaat ataatcgtta tcacttttta aactattgct catcctcact atagattcta
     2821 ttacatttga attaaaatta tccttcatat atggtaaaag gtctaacctt atcttatttc
     2881 tagtgtatat attttctaaa tttgttttat ctatcctcgg atttaaatta taagcttcac
     2941 aatactcctc tatctcattt ctttccacgt ctaatatggg tctaattatg caattatctc
     3001 taatataatc aattcccttt aagcctttta aacctgttcc tctcatgata cgcatcataa
     3061 ccgtttcggc ttggtcattg agattatgac ctattgctat tttattggct ttaatcttat
     3121 ccttaatttc ataaaacatt tcatatctta gcttccttgc tccttcttct aatgataatc
     3181 cctcattttc acaatatttt ggtacattaa tagactttac aaagaatata attcccatat
     3241 cctcacaaag tttagataca tataatgcat ctttttgagc ttctatccct cttatttgat
     3301 ggtttaaatg tgctgcatat atttctatat taaaatcttt ttttaatcta tttagtacat
     3361 gcaataaaca aactgaatca ggtcctccag aaagacctaa aactatttta tcgccttttt
     3421 gtattaaatt atgtttattt atagtactta gtaccttatc aaatatcata tttatccctc
     3481 ttacacttag ttttattcac ttttaagtat actttctata aaaaaacgct aaacccttgt
     3541 atcaatttta agaaattaaa gtttaatata aatataataa taccaaataa attaaataaa
     3601 aggtagtttt ttcactacct ttataaaatt tctggaaaat agccatagtt attatttagt
     3661 acctatgtat atatctattt tattcctacc taaaaataaa tcttgtacct ttatattata
     3721 gtctattttc aattttatgt gttccccttc ttgtatattt atatctaatt ctcttgtatc
     3781 aatatcaatt aataaagtac cttgtggggt cacatacttt gtagtactac tttctccaca
     3841 tttaaacagc attgttgagt taatagcacc aaactttttg atactaactt gatttttaag
     3901 tatctttata gtattagtta tttctatatc ttcttctttt tccttgtaaa taacatatat
     3961 tccatcattt ttataaaaaa ttttaccaaa ggcagtcatc tctatggtgt ccatatttcc
     4021 tttttcatca tattgtctag tatttatact tatttttaca ctcaacgatt tttcctccat
     4081 agattcttca tataaactag tatttttgta tttctacttt ttgaatctta tcaatttttt
     4141 tctttaagaa ctctcttgct atatctgaaa atttttcagg tatatctgat acataatatt
     4201 gataagttcc atgaatttct gtattgttta taatattttg cacctctaaa atctttttta
     4261 aatcctttgc agtctcttta gctggattaa caagttttat atgctctcca acttcttcac
     4321 ctattgttct ctttaaaata ggataatgag tacatcctaa cactaaagaa tctatatttt
     4381 tttctttcaa ttcttgtaaa tatatctttg ctgtcagttt agccacttct gtatttgccc
     4441 atccttcttc aacaataggc acaaataaag gacatgcttt attaacaatt tctatactct
     4501 catctatttt agatatttcc aagttgtatg cttttgaact tatagttcct tcagttccta
     4561 ttattcctac aattttgttt tttgtagaac ttacagctgt tctagctcct gcttctataa
     4621 ctccaattat cggtatatca tatttttctt gagcttcttt taagcttctt gcagtagcag
     4681 tgttacatgc tataactata gcttttacac cttttgtttt taaaaagttt atagcttgaa
     4741 aagtatattt aattatagtt tctttggatc ttgaaccata tggtattctg gcagtatctc
     4801 caaagtatat tatatcctca tttggtaata tcttcattat ctcttttaga actgttaatc
     4861 cacctaatcc cgaatcaaaa actcctattg gtttattgtt catctttata caacacctac
     4921 tttacatttt tgtgtttaac aacaacttat ttatcgctaa aatttatatt tatattcata
     4981 attaatatat tattacctaa atgattatta tagtttattt tacacaaaaa ataaaggagc
     5041 tatcttaaat tagaaaataa tttctacaaa aagacaactc ctttcactac ttaaaaattt
     5101 aaatttattt tgcaaacaca tgatttccta ttctcatata aggattcaat ctatttatcc
     5161 atttgcaagt ggctatttct ggattccaaa aatatactgc tttattagtt gggtcagttc
     5221 cataaagagc ttcctgagct gctttacgtg ctgagtctgt tggtgccatc tttattgaac
     5281 catcaagcac aactgagaaa gcattttttt gatatatgac ttcttctatt gtgtctggaa
     5341 atctagggtc caatactcta tttattacta cagctgctac agcaacctgt ccctcatagc
     5401 tttctcctct agcttctcct gcaaccaact tagataataa catcaagtca ttatcagata
     5461 aatttattac ttctttcttt gctatatttt tttcttgttt gtttactttc ttaatatttt
     5521 ctgttggttc atatgcaaat acttctttaa taggcataaa tataaaagtc aaagctaata
     5581 gtgtagccat cagtttactc cacctgtcca tgattaaaac cactccttac attcttattt
     5641 ttaaaatttt ttctctaaat ccttgactac ttcaagtact ttaaacttaa tttttactct
     5701 acttgttttt tttggtgtat cttgttttat taacttatat tctttatcag ttaaaacttc
     5761 cttcaactta tcatctgtag atttatctat cactccatta gattcatcaa caaaacatag
     5821 tggagggaac ataacacacc accagttttt tccttcacct ttcccaataa taatctttaa
     5881 tgctttatat tcaccagctg gaagaactat attagaatat tgtttagtag ggaaattgct
     5941 atattgtata cctaccttta cactgtaatt atatccattt ttaagaataa tatttttact
     6001 tatttcttcc agactactat attccttttt tataatagct tcactttctt ttatactttt
     6061 tgaattttga agctttggct gtaaatattt tattacttca tctctaactt ttagtttaag
     6121 ttcttggtct tcatctgtat tgctgtttgc tataacatga aatcttatta atttatcttt
     6181 ataatctttt gatatattgt ctactttttt tacctcacca tttattacta ttgtcattat
     6241 tgatatgact gatataagac ttaatattaa tagtcccaat ctaatcttaa tttttctcat
     6301 tgaaaattcc ccctcaagat aaatttatat catcaattat gtccaattaa atttaattta
     6361 ttcataatat tttttaatac taataaaaaa agctatctta aaatgattac aatcatttta
     6421 aaatagcttt ttaaacctta tattttataa gtctttacta atcatagcct tatctatcat
     6481 aaactgctct gctttttcta tatgctctgt agcatacttc ttggatagct cactatcacc
     6541 tttaattata gcatcaagta agtatttatg ctcttcaaca atattatgtg atgtatcata
     6601 agcagacata taggtaactc taaatctata tacttgttcc cacaatgagt ttattaattg
     6661 gattaatttt ttattattag ttgctttaaa tatacattca tgaaattcta catcaagttt
     6721 taataacgca tctacatcgg attccaaagt gcttttttta aattcttctg atatatcttt
     6781 aagcttttta atatcttcat cactaattct ttcagcggca agatatgctg ctaagccttc
     6841 taggcttgct cttacttcta atacatccat aatatccttt aatgacatat ttgccacata
     6901 cgcacctttt ctaggcaaca taacgactaa accttctaat tcaagctttc ttattgcctc
     6961 tctcactgga gtcctactta ctcctaattg ttctgctagt tgcacttcca ttagcctttg
     7021 accaggcttt aattttcctt ctaagattgc ttctcttaaa ttctcaaaga ctacatctct
     7081 taatggcttg taattatcca agttcagttt agttaaattc tccacaaatt tcaacccctt
     7141 tttgactgct attaacaaca tacacctgtt tatatttttt taataattct ttttttccaa
     7201 ttagagcatc ttctttattt ttaaatagtc caaaaactgt aggaccactt ccactcatca
     7261 tagaacctaa agcattattt ttcatcatta cttgttttat atcacttatt tctttgtgtt
     7321 tacctatagt aacattttca agaatattta ccatgctttc tgatacagct tttatatctt
     7381 cagattttaa acactctatc aaatatttat tatttggtct ttttttaata ttattcaaat
     7441 ctaatccttg ataaacttct ttagtagaaa cgaataagtc tggtttgcat atcaatatat
     7501 ttgtatcaca tggtaaacct ttgatgtttg ttagcttttc tccaatacct tgagctaagg
     7561 caggtcttcc tgatatacaa aaaggaacat ctgcgcccaa attaagtcct atttctttta
     7621 attcatcttc agataatcta agttcccata ggtgatttaa acctactaat actgcggctg
     7681 cattagaact tcctccagcc atccctgctg ctacaggaat atttttttct atgaatattt
     7741 ctacaccttt ttttatataa aatttatttt taagtatttt ggcagcttta taaacaatat
     7801 tatcttcatc taaaggaata tctaaactag tgctttttac ctttatttca tcttcgtcta
     7861 attcttttat ctttactata tcgtataaat ctatcgtttg cattatcatt tcaacaaaat
     7921 gataaccgtc ttgccttttt cccaagacat caatggataa atttattttt gctctactct
     7981 ttagtcttat aaaatccatt ctatcctcct tgtttaaaga ataatgcatc actaacaata
     8041 atattatact acaaaaaata aaataatagg gtattataat accctattat acttaattac
     8101 tttaaatttt ttaatattaa tctactaata cgactttttt ttctaaaata agacacttac
     8161 caggcttaat tggttcatca agttttatat cattaagttc tgcaatttca ttttctgttg
     8221 tattgtattt ttttgctata ttccagaaag tatctccttc tttgcatata tatacgatta
     8281 tgctaggagc tttagataaa tcatatacac cttggtcttc tcccttgata ataaaatttt
     8341 ctgctttctt atctaatgct tcagtaaatc tcttaatttt gataatcagg tctatctggt
     8401 ccctattcaa atcaacttcg actttatcaa tacatgctgt attaaacaca gatgctgtat
     8461 cagttagatt atccatttct atatcatgtt caaatggaat ttcctcacta attttataaa
     8521 ccaattttaa accttcaact gggacaaata aaatttcaac ttttatgatt ccttgtataa
     8581 tgcttttatt tccttcaata taactatttt ctatagacat ggttggacaa acgcttacaa
     8641 tatcttttat ttgtatatca tcattatcat ttcttatgcc ttctctaacc ataaatgttt
     8701 cttcactacg tcttaaagtc ttatttaatt gaataggttt gtggtcaaat tttattatct
     8761 tttgaggcga atatgcatct tgtaatactt ctcttgtaac ttcatctgta acttttacct
     8821 tacaacaagc catacaatct atttctaaaa gacctgtgtt gctttcacta ttttgcttga
     8881 aaatatgatt aaaatctgac atagacaaca atacttcttc agtcatacca tcactaactc
     8941 cagggacttc tacaaattga gtaaactcaa tacctactct atccaactct acaagttcac
     9001 cctcatatgt acaagctaac ggatttattt ctaatactcc accaataata actttattgt
     9061 cagtcactct actttctttt actttaacac atggatttaa acttattata gattgaattt
     9121 cctctgtgtt tattgttata gtatctctta tagaactttc tgctttttct attccaacta
     9181 tatcttgaaa acaaatttcc tttctgtgct tttgtatgcc ttccacttgc gccacatctt
     9241 ttacaatgtc taacctttgc ttttcaaaca aacttcccct tatattcata agagctccta
     9301 ctttaatctt tctctcattc attatagtac aatccatatg ctctacttct gaaaataaca
     9361 tgtattccat atcttgaaca acattgtctt tttctattac ttcgtttata tctacttttc
     9421 cgtctacatt agaaatagta tttttatcat ctgcaatata gattacatta taattaaaac
     9481 tacctctgca aagtatcttc ccatctgcca tttcaatttt tttaagtgat atgtatcctt
     9541 ctgtttttac aatttcatat acatctaatt ttttgtctgg tacaactgcc tccgcttcaa
     9601 tgaatgtttg aaattttcca aagtctattc tattgtcaac tttaattaca tctttaatta
     9661 attccatagt ctacctcctt aagtattata aaaatatcac actatattta tatttaagga
     9721 gcaaaaaaaa aattacacct atttttaaat aggtataatt ttaacttatt tgtaatttat
     9781 cttgatctct aaaaacttgc aactttacat tagatgttaa taaatctgag taactataag
     9841 atactctagg atatccatta ctttcatcat ctaactttat aacaaacacg cttggataaa
     9901 ctttttctag tataccctct tttgtaataa tctgctttct tcctttatta gctttcaaca
     9961 atattttctt tcctatatgt ctctctaagc ttactcttat cttatctaga gtttgaacag
    10021 tagccacaat atcaccttct ttaacgttta tatgtttaca taatatcaca tataaacatt
    10081 tttgtcaaat gtttaaacta attattttat atattttttg caaaaatgtc aatactattt
    10141 ttgaagatat acagtataat tatgttaatt tttgctaatg taatattgtt tttggcattg
    10201 cccatctaaa ttttcctctt gtttcagaac caccaattcc cttaacacct gtaatagttt
    10261 gctctataac ctcatcaata ggaactacta catcaattct tgaacaagca attttctcca
    10321 ctataaaaac agggtctaat gcatgtatca ttattcttcc aggcgaactt gcatagtttg
    10381 ctcctgcact tattattttt tcataatttg attggcatgc tcctgcgaat atgacaagat
    10441 tatccaaatt tggttgccat ttacgtgctt cttttactgt tttaacaaaa tttgaagagt
    10501 ttctatagtt actcatatct tctatatctc ctctttttat tgtcattgca tcatgacctg
    10561 ttattactag tatatccgga ttatattttt ctagtagtgc tcttacttct ttatactgat
    10621 tttgctctga aacagctaca cctactgcag gtattccaag ttgagcatat acatccaagc
    10681 aaatttttaa atattcttta tctccatcta tctgtagtac ttttccaggc attccatatg
    10741 tgttaggatt taattgaagt tttggaactg ctctagtcat tcttttttgt ctttctttag
    10801 cttttcttac tgacttatat aataaatttt ctacattttt atctattaat atatctttta
    10861 tatcaggtgc ttttactaat tctaggtcat caatataagc atctgctatt actctaaatg
    10921 caattccctt tagtatagct attttctcat tattttcatc tacaccaaaa gagactattt
    10981 taaatactat atctttgtta tgtgactttc tagctacaat gtctcctacc ttcatagtta
    11041 aacctccaat aaatttttat cttagtttat actatgttcg atataaaact ttgtttccta
    11101 atgtcaaaaa aacatttctt ataatacttc ttttatcact cttaaagcat ttttatagta
    11161 tatcttttct atatcatctt cactaaaccc ttcttttttt agaggttcat acagtttacc
    11221 catctgagat atatcttcaa tctcaacttt tgaatctatt ccatcaaaat ctgaccctag
    11281 agatacaaca tctattcctc caacatttac aatatgtttt atgtgtctaa ccatatcttc
    11341 aagtttactt tcacttttat cacttaaaaa taagtggaag aagtttatac ctgtaactcc
    11401 acctttattt gctaatactt taatcatatc atcagttaaa tttctagaat gattcatcat
    11461 tgctcttgaa ttagaatgtg tagcaattat tggtttagaa gaaatctttg caatttcata
    11521 aaatccacca tctgaaatat gagaaacatc aaccaacatt cccaattcat tcatcttatg
    11581 taccacttct cttccaaaat cagtaagccc tttttctcta tattctactt tattatgagg
    11641 aaagctcaat tcattcacat gattccaact tattgtcatc attctaaccc ctaagtcgta
    11701 aaattttttt aaattttcta atttaccttc aagtactgca ccttcctcaa tagataaaag
    11761 agctgtaagt ttaccttctg attggttttt cataatttct tcatagttag tagctaaagc
    11821 tatttcatca ctattttttt tcatttcctc atgaaattta tttgccatac tcatgcaata
    11881 atcaaaagga tgttttactt cttcagtatc aacaaaaaga gcaaatgttt gagctaatga
    11941 atcccccttt ttcaatctgt ctatatcaac agaatattta ttagacttaa gttcacttgt
    12001 ttctacattt tccattaatt ttgctatagt atcacaatgt aaatcaataa atttcatatt
    12061 tataccccct gtatatttta tatttatatc taaaaataga aatagttgtc cttatagaca
    12121 actattgaca actatttttt aatattcttt ttaatattac ttaatcttat tcttctttta
    12181 agttaggtat tactttttta acttcttcaa tttgatgcca tgttttatct atattatttt
    12241 cactaatata tttttttaaa tcagttaaag atgtaaaatc tttaactgat aaattgtcat
    12301 catcatctgc aaatcttaaa ataaacatga gactccctta ctttccacaa catttcttat
    12361 atttttttcc gcttccacat gggcatgaat catttcttcc tatttttgct tctttaacta
    12421 ttgttttaga cttcttataa gcctttatta tttcttttct ttggtctgct ggtagtatat
    12481 cttcccaacc ttgtaaatta tatagccaat gagcctcaac agttaccata ttgttatata
    12541 atttttccca atctattttt atagaaactt ctttatcagc ttctacttct tctaaaacta
    12601 tatcttcttt taagctttca cttattccat ctatgaatcc catgaaaaat tcattagtag
    12661 ttccaaattt ttcagccaat tcacttactt taccttcaac aacatccatt ttattattta
    12721 atatttcatt gtatattcca gcttcaactt tcaaatattc ttcccagaat tttacttctg
    12781 cttcttgact ttcatgatta tcacttaaat ttctccattc agtatataaa ctcatacttt
    12841 ttcctcctaa gtatacctat atttttatag tttttatagt ataaaatctt ttagttagat
    12901 tggaagtttc ttctttttga ttataccata ttctatttaa agttaactct tatattatat
    12961 cataattaaa caattctttt aatactttta tagcatactc ttggtttata aaataagggc
    13021 ttgtcgcaat tgttattact ttggcttttt taattaagtt ttttatcttg taaaatttga
    13081 gttcaaatgg tatatagtcc atatcacttg aaaatatatc caaatctatg tcaagaacaa
    13141 actctgattc tacttctaaa tcaaatccat atagactatc tattattatt aattctgaaa
    13201 atatgttata ttttaaagca ggttttataa aacttcccac atttaaaact tcatttgtat
    13261 atccaaatac atcttttaga ttatttacat ctacatcata gttctcaggt tctctagtat
    13321 ccttatgttg gtctacatgc actaacttgc atccttttgt aaactcaccc tttaacaaag
    13381 attttgccca aaaataaaat gcatggttat gattgtcaaa cacatatatc tttttatttc
    13441 ccagggcata ttcgaccata ttctccaatc cttttgcttt tatttcctta ttttcatcta
    13501 cttcattaaa tactacctca ttgcctaacc ttacatcatt taatgtacca tttataagtt
    13561 ttggaacgta tatgctctta ttttctcttt tatcatatga aaatacatta tttcctactg
    13621 gtttttctat ataaaatcca ctatattcat aattttccat aaaactttat ttcctccatt
    13681 gttatataaa tatataagtt ataattttaa aaattaaaat gcacttattg taaatcatat
    13741 cttctattat agaaaacatc ttatataact aaatataata tcttctctca atatgtatta
    13801 tacaataagt gcaaaatata tttatataaa atatactaca ttcctataac aaaaccatca
    13861 gcttcaattg gaaggtaact aactcttcta ttcaactcca cctttccaaa aaatactacc
    13921 caatatggtc taaaaatact ttcaatttta actatttcaa tctctgggat tcctcccata
    13981 aacctatgag ttaatctcat agccatttta tttgcctttg cttttactct tcgttcgtcc
    14041 ttatcactat actgcacaat attacaatcc acatttttta caacttcata tgtaggaaaa
    14101 tcttgtacta tactaccaga acctgtagaa ccattcacta aaatttttaa ttcatttata
    14161 tcattagaat atctcctatt cttgaaaatg gatttcgttt tgtgaatttt aagtgtgata
    14221 attttaaact ctatataatg aagtctaact tcatgagcat atttgttctt taatattagc
    14281 ttactaaata aagaattttt tccactttta gctatctcta tagcttcttc ttttgtaatt
    14341 cttataggta acgctcttat atccataaaa tcattctctt tcttattttt ttcttgtcat
    14401 ttttaaaact ctatatatat aatcaaaaaa tattacaatt acaactgcca taactccata
    14461 tggaattatt tttccaacta taatatcatt tctagtcaat ataccataaa atataaatcc
    14521 tgttattaaa aagtacaata atactagaaa accgtctatt ccaagctatt ttatttctaa
    14581 atcttttttt accttagaca ctaatttaga catagcagtt tctcctttta tagattattt
    14641 tgtgtattta aattttcacc tattctatta ccaatcatcc atgcaataaa tgaaaaaggt
    14701 atagctatca caagaggctc taactcaaag ctctgaactg gaaatatttt aaaataacca
    14761 aaagtcactc caccaacaat cattcctaaa ataccaccca tagatgttct tcttccattt
    14821 ccaagaagcc cacctactat aggtacaaat gccgaaccca tccaaatact tcctacaaat
    14881 atacatgcat catatacatt ttcaaatcta aatgctacgc taagccccat aactgctgct
    14941 aaacatacac ctattctagt aaactttact aattgtttct gagataactc tttttttgta
    15001 attcttccat atatatcatt tgctaatgta gctccagctg ctagataata gctatccaat
    15061 gttgatatta tagaacctaa aattcctatt ataaatatta ctcttattcc ttgtggtaat
    15121 gtatctaaaa ctaaagttat atatccagca gctggagcca tttctggata agtagctctt
    15181 actataagtc ctgtcatagt taacaccatg tcaaaacata tccatagtga aaaacaagtt
    15241 aataaggctc ttcttccaac cttaggagaa tctgctgctg aaaatctttg ataaaaagtt
    15301 gggtctgaat aagctccaaa agaatataca attaacatca atgctttcat agctgacata
    15361 ccttttgtag ggtgcataag ctgtggtgca tctttgagtg cttctgtaag accttgaaac
    15421 cctcctattt gttcaaattg acctggcata accatagtta aaccaaatac catacaaaaa
    15481 aacaatacca tatctgtaat ggcaactccc ataagcccac caagtactgt aagtacaacg
    15541 actactccta ccattattaa tcctattaat tctttatcta ttttccatgc tacgttacct
    15601 atagttccca tagcagttat ttctccaagt tgtgcacaat atataaacat caaaattgcc
    15661 ccaattatag caacatgctt gccataaaaa ctttccagta aatcaggaac tgttatattg
    15721 gttcttatat gaatccttgg accaacccac aatgctaaag gcattctccc aatatgtgca
    15781 gctattgacc aaactgccca tgcagatatt ccatatatag ccgcatactc tacactacct
    15841 actgtaccaa ctcctccata ccatgatgca accaaagttc ctactattaa ggaccatggt
    15901 aaacttcgat ttcctaggta ataactttcc atatctttta catttctaga aaaataataa
    15961 cctattccta ataatataaa tgcaaatata actataatcc cattttccca aaatgttaac
    16021 atatctaatc tccacattct tatattttaa tttttaactt taatatctaa atatgaacaa
    16081 ctctacccat ttccataaaa ataggtagag ttatataata attataagta tttaagtaat
    16141 cttgttatta agcaatttta ttgtttaaac ctttattttc tcttttattt attttattta
    16201 aagcataagt agcaaatatc cctataactg cacatactac agataaaata aatatatatg
    16261 tatatcctgt ttgtccatat ttatctaacc aattacctgc aatagtgtaa taaaacgcat
    16321 ctggagaaaa tcctataaat gaagcaaatc caactactgc tcctgtagtt tctaaagata
    16381 tattagactc tgaaacagat gcaaagtata tacctctaaa tccaaataaa attacactta
    16441 gtattatcat gtttattaca gctatccata taaatcctgt actcttagga ataaccaagt
    16501 aagcagctgt acttactgtc attaatataa atcctacaaa cattactctt atagatgaac
    16561 caactttatc tgttataaca ccagcaactg gagatgctcc catttttata acataagttc
    16621 ttattattga taaagcagaa actagagtca ttggcattac ataaacttct gataagtatg
    16681 gacttaaata agttaaactt gagaatacta aataagttga aaatattata agaccaatta
    16741 accaagcctt tggcattgta actactttta acaatgactt aatacttaat ccttcatctg
    16801 atgctccact atcttttttc tcttctacta agaaaaatag aagtatacca catattatag
    16861 atgctgcaga atataaccat actacatatt tgaatcctac tgttatttca gcaaatttac
    16921 caaagaaata aagacctata aaagaaatta atgtacctga tacgcctgat aacccttcat
    16981 aaaaaccaaa tattcttcct tgctctgact cacttccttg cattctaaca accttaacag
    17041 aggctgagaa gaaggttaat attgtcgtaa atccccataa tacaaatatt aaaagtatca
    17101 tactatatga tgggaatgta gagaaataaa atccaagtat tcctgaagca attaaagaaa
    17161 aagttattaa ttttctaact gagaatctat ctgcaaacca tcctcctata aaataagata
    17221 ccattcctac taatccatat aaactcaata agttccctat ttgtgtatga cttaatgcta
    17281 aagcttgttg cattgggtca taaaatgatg atttcatata tggtaaacta tacatagctg
    17341 ttgttccaaa tgccaatatg aatattatca aaaatctttt aacattattt ttgctttcca
    17401 ttgattctct ctccttaaaa ttttattgtt tataatctca ttcatgtaat tttattttaa
    17461 tttttttatt gcttgtttaa agtcatttat tatatcattt gaagattcta atcctacagc
    17521 tattcttatt agattttccc cattcatttg aactaatgta tgctcttccc ctaagcttgt
    17581 tgctattgtt gcaagctcta aagaattcat taatcttcta ctagcttggt ctcttgtcaa
    17641 tccattgata ccatccttaa gtttaaatga aactacacct ccacctaaac cattcatttg
    17701 agttttacaa agctcatatt gaggatgaga ttttaatgct ggatacttta ccaattccac
    17761 attctcttct tcctctaaaa actctgctac tgctaatcca ttttcacaat gctttttcat
    17821 cctcatatct aacgttttca tacctcttaa aatcatccat gcactcattg gtggtaaaga
    17881 tgctcctgta aaacaacata aacttggcca tctaactaaa tctattaatt cttttgaacc
    17941 tactactgct ccccctaaag tatccccatg tccatttata aacttagtaa gactatgtat
    18001 tactaaatct gcacctaaat ctattggtct ttgtacaatt ggtgtcccaa aagtactatc
    18061 aactatagct aagcttttat tttcatgagc aatttctgct attgttttta tatctactaa
    18121 gtctaatgat ggatttaaag gactttctat ataaactatt ttagtatttg gtctcattgc
    18181 ttcttttaca ttttgtggat ttactgcatc tacaaatgta acttcaactc caaatttaga
    18241 taacaactca gacatgaata atttagtgtg tgaaaatatt ccatctgaac taactatatg
    18301 gtcaccactt tttaccaatg ctaatatagt agatgttatt gcccccatcc ctgatgtact
    18361 agatatggca ctttctgcat tttcaagcat agccaactta gcttctaact ctgtaagagt
    18421 aggattacac tctcttgtat acacatatcc ccatgtcata cacaactctt caaaatgctc
    18481 tactgtcttt gcagcaaatg ttgctgtttg acatattggt gtagccaatg cacctgttgt
    18541 tgggtctgca ctatgtcccc aatgtattat ttttgtctct aaatcttgat taatattgtt
    18601 actcattttg atttcctccc attttgactt atttatacaa cgttttccct gccaaatttc
    18661 atcaatttag cttagtctta atacattttt tcaagtatac ttttttattt gttataattt
    18721 ctaaaaatat gacacttatt ttgttttttt acagttctaa ttataaactc tatagtaact
    18781 atagagtcaa tatgtttttg atattttttt tacaatgttt aaaatagtca gtacaaaaat
    18841 aggtatattt tagaataact aaagtcatcc taagatatac ctatttttat atcacaatat
    18901 aaataattac atataagcat tagttttttt atcataaata gatttttc
//
LOCUS       DACXMZ010000060        18683 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.060, whole genome shotgun sequence.
ACCESSION   DACXMZ010000060 DACXMZ010000000
VERSION     DACXMZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18683
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.060"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            194..427
                     /locus_tag="JD016_08180"
                     /pseudo
     CDS             194..427
                     /locus_tag="JD016_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417537.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(551..2311)
                     /locus_tag="JD016_08185"
     CDS             complement(551..2311)
                     /locus_tag="JD016_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAU4999616.1"
                     /translation="MQEVEVIKAKNVLARQARGKAIERLRVAAYCRVSTDSEDQLNSY
                     KSQVQYYTDMIKKNKEWVLADIYADEAITGTQVTKREDFQRMINDCMNGEIDMVFTKS
                     ISRFARNTLDTLKYVRMLKERNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMKRGELVGFQGCIGYDYHPEDKTITVNQEEAEIVRYIFNRYVDGAGGS
                     VIGQELESLGYKTKYGSSTWAPSTVIGIIKNEKYKGDILLGKTFTVDPISKRRLENMG
                     EEDKFYMKDHHEPIISEEVFEKAQEILNRRNKNRTTVGSGKREKYSRKYAFSCMLECG
                     FCGGTLTRRNWHSGSKYSKVIWQCVTATKKGKKFCKHSKGIPETAIEEAFVESYRLLC
                     DDNKDVLEEFLQRMDDTLSSSVVSKQLAKAEKEIDALEKKKSKLVDMRLEEIIDKETY
                     ESKYADLVSKQEQLVEERQKLQETSDNEKDIKKRLKEFKKTLEQNEVLDKFDRYVFES
                     IVEKVIVGGLDENGNVDPAQLTFVYKTGLKNSVDGAKFKTQRKNARGRHRTNELCSHD
                     SNEVDKMCSDSSNDTCGNCS"
     gene            complement(2374..2517)
                     /locus_tag="JD016_08190"
     CDS             complement(2374..2517)
                     /locus_tag="JD016_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU4999617.1"
                     /translation="MNNIIRYSMQIAMLKQLLARKLISEKEYTLVKQRLMKDYKVLSD
                     ITA"
     gene            complement(2836..3075)
                     /locus_tag="JD016_08195"
     CDS             complement(2836..3075)
                     /locus_tag="JD016_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU4999618.1"
                     /translation="MMKSTKKCPSFSTISSAADGNEVAIEKILNHYDAYISKASLRPF
                     YDEHGNMYIVLDMELKGRIRAALIKVILGFEVKVK"
     gene            complement(3080..3496)
                     /locus_tag="JD016_08200"
     CDS             complement(3080..3496)
                     /locus_tag="JD016_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAU4999619.1"
                     /translation="MKPSSFEGAIRLQFDCLARKVVGRTVKNYDRELGRRARRETPFC
                     DLSEMELNQIGAMDEYCVEFTSFDVFGSEVHIYDERLCEAIKELTESRRNILLMSYFL
                     EMTDAEIAEVMEMERFSVCRNRLHTLKLIKDMYEEE"
     gene            complement(3493..3852)
                     /locus_tag="JD016_08205"
     CDS             complement(3493..3852)
                     /locus_tag="JD016_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU4999620.1"
                     /translation="MPNVGQITGWSCFIGSRGSRMKHLHIQQLSTIGSSLKKHLNSTE
                     HNAPICGFLQHITGIICLAQDQYVCIALVLFYCIKLFQKIFAVGYQIPPFMVSVSAKR
                     GRSLFALATFAGKGVRL"
     gene            complement(4843..5013)
                     /locus_tag="JD016_08210"
     CDS             complement(4843..5013)
                     /locus_tag="JD016_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999621.1"
                     /translation="MMNWKELWMTLFGTTEWLGLNIGFWVAMAVVLLIVIVMNVVFWS
                     MKPKKDTKESYK"
     gene            complement(5023..7788)
                     /gene="mgtA"
                     /locus_tag="JD016_08215"
     CDS             complement(5023..7788)
                     /gene="mgtA"
                     /locus_tag="JD016_08215"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAU4999622.1"
                     /translation="MNKKENRMAIRQTVEKAVIRDEQNRRIQFAATNPIKEVLKKLHT
                     TLRGLEVDEVSVSRAKYGTNKVTHEKKKSLAKRLVGAFINPFTAILFCLALVSTITDM
                     IFPYFSLFGSVPEDFDSLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNYEKKELPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTNIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLAAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEAADSRLIDLSENYVKVDEIPFDFTRRR
                     LTTVVQDNKGKTQMVTKGAVEEMLSICAFAECNGGVQPLTDDVRYRILETVDNLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECDMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVAESTDVFAKLTPDQKARV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIFLNLIYDLSC
                     TAIPWDNVDEEFITKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFVQSRAS
                     APVTLLTMTGIIVLTIIPFTVFGTMLGFVALPAAYFAYLIPCILLYMTLATSLKKAYV
                     RHFGELL"
     gene            complement(7954..8265)
                     /locus_tag="JD016_08220"
     CDS             complement(7954..8265)
                     /locus_tag="JD016_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HAU4999623.1"
                     /translation="MDFTCIFFGILFTLAGFWLAFGKGYRHLSLWKNMPQEEKDKINI
                     VPLCRNVGEMIALNGIIFLMKGFLPGFSNHWFVFAIIAWLIVAGFDVWYIEKSVRYRN
                     Q"
     gene            complement(8281..8511)
                     /locus_tag="JD016_08225"
     CDS             complement(8281..8511)
                     /locus_tag="JD016_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU4999624.1"
                     /translation="MRREIEDLKRAILFAFYPVFTILCSYLGWNFFEQRMVIFKIWYQ
                     VSIVIWFILAVTIIVLFIKLLKLTKLRNRFKH"
     gene            complement(8512..9189)
                     /locus_tag="JD016_08230"
     CDS             complement(8512..9189)
                     /locus_tag="JD016_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAU4999625.1"
                     /translation="MFYTDFILRIALSLTLGFLIGLERQLTGHPAGIRINVLICMGTS
                     FFTLFPMLYGSDQVFRVGSSIISGVGFLCSGVIFKDRGTVRGMNTAATLWCTAAIGIL
                     ASTGMYAMAITAATILIGSNLILRPFARKFHPITSSDESEKQYRISVTCQEKAEQDIR
                     LLLINSNSCKTLFLNNLESGDVVGDKVEIVAEYCSVGKSKNNILEGIVGQVLVIPEVI
                     SAGWEVL"
     gene            complement(9327..9830)
                     /locus_tag="JD016_08235"
     CDS             complement(9327..9830)
                     /locus_tag="JD016_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAU4999626.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDCAKDLLSGIQEMIR
                     GEVSSLLLGASEESDWPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWEEDYILDNSNIMAFIRKLRKKIEPAPDCPTYILTIWGIG
                     YKFNDQI"
     gene            complement(9846..12509)
                     /gene="mgtA"
                     /locus_tag="JD016_08240"
     CDS             complement(9846..12509)
                     /gene="mgtA"
                     /locus_tag="JD016_08240"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAU4999627.1"
                     /translation="MSKATLFDSRIKKYAYCRTSEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFVASNFARNATTAIII
                     FSMIVISGVIRLIQELRAKSAAAQLDRLVHESVTVRRDGKLIEIPGEELVVGDIVLFS
                     AGDRVPADIRLTKITDLFISQAAITGESAIIEKSCRKLSYGEQDTLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKNSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITDGKWMESFAFALSVAVGLMPEMLPMVITACLAKGSLAMSKKQTIIKDINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDIFGNENAEVLDLAYLNSCYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNHVEYRGKLLPMGQDARQSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPDDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGIYTERILTG
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEQGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEMLSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLQLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIAFTSLTFVGGASLFGLTKLPLW
                     YFVFLLLVVSLYMLLNSVAKYFYKKKYQELI"
     gene            complement(12602..12811)
                     /locus_tag="JD016_08245"
     CDS             complement(12602..12811)
                     /locus_tag="JD016_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999628.1"
                     /translation="MVKVKTKKKFVSTHMCQGEMPIYVRERYEKMLAVSIRAPNLEGM
                     VENFDKDLSRPNETDLIELKNAISK"
     gene            12761..13096
                     /locus_tag="JD016_08250"
     CDS             12761..13096
                     /locus_tag="JD016_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999629.1"
                     /translation="MTHMSADKLLLGFHLDHVSNLNELLRKVAHNNICIMSDRMTKVA
                     VWKPSVKTIQLVCMEYLNYIIDHSVIYEKIQCANWNGTEETNSIFNNSPSPLITLIYS
                     CDLQQNKTT"
     gene            complement(13295..14197)
                     /locus_tag="JD016_08255"
     CDS             complement(13295..14197)
                     /locus_tag="JD016_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU4999630.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDQHTTHEKEVIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIDARTQAISG
                     YLTKELQDLNMDTIRTDIPTSSAITDVIVWSIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDAGGNMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTINDATAFLE
                     TFFKLYPTATEKELAYYVSGNVLESIGRDYLYSELVNPIFTVDGDNVKVKVAVKFIDN
                     QTKATQVSQFELVLHKESNWKIIG"
     gene            complement(14214..15221)
                     /locus_tag="JD016_08260"
     CDS             complement(14214..15221)
                     /locus_tag="JD016_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAU4999631.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPSS
                     LDTESSIKQGCKYFASLLSSCKNQDIDDLNVAIQSYNYGGGYVGYVAGNGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVFGGSSPTTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYTDLSSSYWQQHLIGAGRVK
                     Q"
     gene            complement(15218..17422)
                     /locus_tag="JD016_08265"
     CDS             complement(15218..17422)
                     /locus_tag="JD016_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAU4999632.1"
                     /translation="MKERIQSAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFFVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRSERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHKKSIAVKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASTVSKTGAEKMQEVKRPATTPTTKASEPVKTSGVKERPLSSSTPDGKATQSTYR
                     QNVEKVTVSKETRQNYTKDRRTKVQQTQTVQKNQQTTEKIRNLVTKKGQKKK"
     gene            complement(17422..>18683)
                     /locus_tag="JD016_08270"
     CDS             complement(17422..>18683)
                     /locus_tag="JD016_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAU4999633.1"
                     /translation="PASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDT
                     GRNVYLQPSLASQGIKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDP
                     KSERGNWKETLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTG
                     ISSRDGEKFPVLRKAVRSVTQSDSRGLLHVIDELRREDTPVSRNIADHIDSFTDYDFA
                     HLLFSDGTVENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFAL
                     DFIHSDRSIFKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKE
                     SLKNNIGLKFAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVG
                     VVQIHPVFEELLHAFDTRPPVQRNEVE"
BASE COUNT         5527 a         4224 c         3269 g         5663 t
ORIGIN      
        1 acgtagtttt aattataaaa aaagatgaac aaaaattata cagtttaata tggaatcagt
       61 aggtacatat gtattattaa gaattataaa taataaaaat aaattttagt caatatacaa
      121 aaacctttta aattgtctgg aaatcacctc ttcatttgtt ataaaaacat acaacaaatg
      181 aagaggtgat tttatggttt taaaaaaaat aattataagt ggatatgtgc tttcacatgt
      241 ttatcaacat ttgtaatgcc tatatttgct aatgaattat aaaaatatta aataatattc
      301 aactatagat tatgtaaatt actctatgaa aaaaatgagt tgtataaaat ctggctactg
      361 tagattgact atttctttta aaattacaat tataataagt tttatagaat taaaaataaa
      421 actttagaga tttttgataa cattgtagac taaatttgcc accgaaatcg ctttacagtg
      481 gcaaatcggt ggcaaataca atatattatt agtatataat ctatataaac attactattg
      541 tttgattatc ttagctacag tttccacatg tgtcgttact actatcggaa cacattttat
      601 caacctcgtt gctgtcatgt gaacacaatt cattagtcct atgccgtcct cttgcatttt
      661 tcctttgtgt tttaaattta gcaccatcta cgctattctt caagcctgtt ttataaacaa
      721 aggtcaactg tgcagggtca acatttccgt tttcatcaag tccacctaca attacttttt
      781 caacaatgct ttcaaacaca tagcggtcaa atttatcaag aacctcattc tgttccagag
      841 ttttcttaaa ctctttcaaa cgttttttta tgtctttctc attatcagaa gtttcttgta
      901 acttttgtct ttcttcaaca agctgttctt gtttacttac taaatcagca tatttacttt
      961 cgtaggtttc cttatcaata atttcttcca gacgcatatc cacaagttta ctttttttct
     1021 tctctaatgc gtctatctcc ttttccgctt ttgcaagttg ttttgacacc acactactac
     1081 taagagtatc gtccattctc tgcaaaaatt cttccagaac atctttatta tcatcacata
     1141 ataaacgata actttctaca aatgcttctt caatagctgt ttctggaata cctttgctat
     1201 gtttacaaaa tttctttcct ttcttcgtag ccgttacaca ttgccatata accttgctgt
     1261 attttgagcc actgtgccaa ttacgtcttg ttaaagtacc accacaaaag ccacattcta
     1321 gcatacagct aaatgcgtat ttcctgctat acttctcacg cttaccgctg cctaccgttg
     1381 ttcggttctt atttctccga ttaagaattt cctgtgcttt ttcaaaaact tcttcactaa
     1441 taatcggctc atggtgatct ttcatataaa atttatcttc ttcacccatg ttttcaaggc
     1501 gacgtttgga aatcggatca acagtaaaag ttttgcctaa gagtatatca cccttgtatt
     1561 tctcattctt aataattcca ataaccgtag atggtgccca tgtggaactc ccatacttcg
     1621 ttttataacc tagactttca agctcctgtc caataacaga accaccagca ccatcaacat
     1681 aacggttgaa aatatatctg acaatctctg cttcttcttg atttacagtt attgtcttat
     1741 cctctgggtg ataatcatat ccaatacaac cttgaaaacc taccagctcg ccgcgcttca
     1801 ttttcatttt caaccctttt tttacattgg ctgaaatgtt ctcgacttcc tgctgtgcaa
     1861 cagaactaag aacaacaagc aataactctc catccattgt aagtgtattg attttttcat
     1921 cttcaaaata aacggcgata tttctctctt ttagcatacg aacatatttc aatgtgtcta
     1981 aagtatttct ggcaaatctt gatatggatt ttgtaaatac catgtcaatt tcaccattca
     2041 tacagtcatt tatcatacgc tgaaaatctt ctcgttttgt tacttgtgtc cccgtaattg
     2101 cttcatcagc ataaatgtca gctaacaccc attccttgtt tttcttaatc atgtcagtat
     2161 aatactgaac ttgtgattta taactgttta actgatcttc gctatctgta cttacacggc
     2221 aatatgcagc tacacgcaaa cgctcaatgg cttttccacg ggcttgtctt gccaaaacat
     2281 tttttgcttt tattacttca acttcctgca tagtacacct ccttattttg tgttcctttc
     2341 atattgttat tgtactactt taaaaaacac aattcaagca gttatgtcag aaagcacctt
     2401 atagtctttc atcagccttt gttttacaag agtgtattct ttttcagaaa tcagtttacg
     2461 ggcaagcagt tgttttaaca tagcaatttg catactatac ctaattatat tattcattaa
     2521 aaccctcctt gcttttttat ttatggaagt aatcagacgg cttcggcagc tccactggac
     2581 tttcaccatt cccattctga tgggtgaacc gtgtcatacg ggtgtatcat tattcagata
     2641 tacgagtcat ggcagcaacc tttccaaaga tcgcttgcgg ttcactggca taatcgctcg
     2701 ctgctttccc catttcagtg gtcgtggcgt tccagtccgc tggctcgtcg tatatcaaac
     2761 gtatctgatt actgtattca cttgtcaaag aacggaaaag agttaaaatg aggtggtaga
     2821 tactctctgt tatatttact tcactttgac ttcaaaccct aagattactt taatcaacgc
     2881 tgcccgtatc ctgcctttca gttccatatc aagcacgata tacatattcc cgtgttcgtc
     2941 atagaacggg cgtaggctgg cttttgatat gtaagcgtca taatggttca aaatcttttc
     3001 aattgccacc tcgttaccgt cagctgcaga actgatggtc gagaatgatg gacacttttt
     3061 tgtagacttc atcatgtttt tattcctcct cgtacatatc cttaataagc tttaatgtgt
     3121 gcagcctgtt tctacatacg gaaaatcgtt ccatttccat aacctctgca atttcagcgt
     3181 cggtcatttc aagaaagtag gacataagca gaatgttacg tcggctttct gttaattctt
     3241 tgatagcttc acataagcgt tcatcataga tatggacttc tgaaccaaat acatcaaatg
     3301 acgtaaactc aacgcaatat tcgtccatgg caccgatctg gtttaactcc atttcagata
     3361 aatcacagaa cggcgtttca cgtcttgcac gtctgccaag ctctcggtca tagttcttta
     3421 cagttcgtcc tacaaccttg cgagctagac aatcaaattg aagtctgata gcgccctcga
     3481 aagaagatgg tttcataatc tcaccccctt tccagcgaaa gttgctaaag cgaaaagact
     3541 tctacctctt ttcgcactaa cactcaccat gaaaggtggg atttgatacc caaccgcaaa
     3601 aattttttga aaaagtttta tgcagtaaaa aagcacaagg gcaatacaga cgtactgatc
     3661 ttgtgctaaa caaattattc ctgttatatg ctgtaaaaaa ccacatatag gagcattatg
     3721 ttccgtagaa tttaaatgtt tttttaatga actaccgatg gtcgaaagtt gttgaatatg
     3781 taagtgtttc atacggctac ccctagagcc gataaaacaa gaccatccag taatttgtcc
     3841 aacgttaggc aaaaataaaa cggcggctat ataaccgtcg tcttattcaa aagttcaaaa
     3901 agaactaaac taaaccctct aaagcagtat gtatttataa tcagtcttgc aaaataacaa
     3961 agtagaattg aaatataagg tagcaatcct tgtgcatcta ataaacttat aggggttaca
     4021 gcagaccacg gaataatatt ttgtatactt tcagatcaat atagtccaac aaaaccaatt
     4081 cccgatattt agtaactaaa aagtaaacta gagtagaaaa catttctttg caaaacacaa
     4141 ataataagta atggactcat tgacaataga ttgagaaatc cagtttttta gacttagata
     4201 gattatctca cttgtaaatg atttaagttc tttgacttca tatatagcgg tataatctgt
     4261 caacgtgatc attacaaacc ataatgccta tataacatac tttccaagca aaaactttat
     4321 tctattagtt ggggaataca aaatatttat cattgtgcgt tcaacatatt ctttttgtat
     4381 aaaaatgtaa ttacaaaaca ccactcaata taagatagat aataatttga ataggaatta
     4441 tcaatctacg taaccattca ttacctgtca ttgttgttac aatagatttg ggattctgtt
     4501 tccattacat aattttttta tccttctttc agatatctcc tcattttatc gaagcttctt
     4561 ctacagacat acattccata ataaaatcta cctttgaata ttatttcacg atttcgttat
     4621 agacgcgtaa acaaacaata aaaaacgaaa tttcttttaa gtaccataat ttttatatcg
     4681 aaattacaat tatccactta tatacagcat ttataggtaa ttcaatgata aaaaaacaca
     4741 tactttacta ctaaattgcc acaattgaat gcgccttgat atgataggga gattccacaa
     4801 taaaaaactg tgcatctccc ttgattcatt catactgata tcttatttat aagactcttt
     4861 tgtgtctttt tttggtttca tggaccaaaa tactacattc attacaataa caatcagcaa
     4921 cacaactgcc atagccaccc aaaagccgat attaagacca agccattcgg tagttccaaa
     4981 aagggtcatc caaagttctt tccagttcat cattccacct ccttaaagca gttctccgaa
     5041 atgacggaca taggcttttt ttaaacttgt tgccaaagtc atatacagca gaatacatgg
     5101 aatcagataa gcaaagtatg ctgcaggcaa tgcaacaaat ccaagcatag ttccaaacac
     5161 agtaaacgga ataatggtca gaactataat tccagtcatt gttagcaaag tcacaggagc
     5221 tgatgcacga ctctgaacaa aaggtagctt tggagtacga atcatgtgaa tgactaatgt
     5281 ctggctccac atagattcca cgaaccaccc tgcttggaac atcccgatat actgtgcctg
     5341 aagcattgcc agttctgcac cgctgaaatg agcaggcaaa tcattaaata atacaccatt
     5401 cgatacgaac aatggacaaa atacaaagta cataaaaata taggttgtaa aatcaaagat
     5461 agaactggtt ggtccaatcc agatcataaa acttccaaca ctggaagcgt cccatttacg
     5521 aggtttggta ataaactcct catccacatt atcccaagga attgcagtac aggataaatc
     5581 ataaattaag tttagaaata taagatgaac actcatcatt ggtaagaaag gtagcaaagc
     5641 agatgcagca agcactgaga acatattgcc gaagttggat gaagcagtca ttttaatgta
     5701 cttgatcatg ttggcatatg tctttcgtcc ctcaataata ccctgttcca aaaccatcag
     5761 atctttttcc aatagaataa tgtcagcaga ttcttttgcc acgtccacgg cggtatccac
     5821 cgaaatacca atatcggcgg ctttcatagc tgcagcatca ttaataccgt cacccatgaa
     5881 accgaccgta tgaccatttt cacggagaac agaaaccaca cgggctttct gatcaggggt
     5941 tagctttgca aatacatctg tagactctgc cactcttgca agctctgcat cgcccatttg
     6001 ttccaaatca gaaccgagaa gcatattgcg aacctctaat cccacctgct tgcagatagt
     6061 tcgagtcact ttgtcgttgt cacctgtcaa aattttggtt ataacaccat ggtctttcag
     6121 agctttaata gcatccgcag tagattcttt tggaggatca aggaatgcca gatatccaat
     6181 cagtaccata tcacactcat ctttaacacc aaatgcgccg attggggaag gattactctt
     6241 ctgtgcaata gctagtacgc ggaatccctt atcgttaaga ttgtccactg tctccagaat
     6301 tctgtaacga acgtcatcag tcagaggctg cactcctcca ttacactctg caaacgcaca
     6361 aatagagagc atttcttcca cagcaccttt tgttaccatc tgggtcttac ccttattatc
     6421 ctgtaccact gtagtcagac gacgacgtgt gaagtcaaaa ggaatctcat ctactttcac
     6481 atagttttca gacaggtcaa tgagacgcga atctgcggct tcctcttcct ccgtcttatg
     6541 gataattgcc aaatccatca ggtttttata gccagtctgg aagtaactat tgaggtaagc
     6601 gtgacgcagt actcgcgtat cgtcctcgcc atttacattc agatggtatt ccagcacaac
     6661 cttatcctgg gtcagcgtgc cagtcttgtc agtacagaga atatcaatag caccgaagtt
     6721 ctgaatggaa ttgaggtttt taacgattgt ctgcttcttg ctcatggaaa cagcgccttt
     6781 cgcaagacag gttgttacaa tcatcggcaa catttctggt gtcaaaccaa cagcaataga
     6841 aacgccgaat aagaaggccg ccaaccaatc gcccttggtg ataccattga caaagaacac
     6901 cagcggaacc ataatgagca taaaacgaat caatacccaa gaaacggcat tgacaccctt
     6961 ggtaaagctt gtttcaaccg cttctccagc aactgcggac gccatagagc caaacagagt
     7021 atgatcgcca acacaaacaa caacagctgt tgcgctacca gaaattacat tactccccat
     7081 aaaagcaatg tttgtgtaat cagtcacgct ttctttctga gcacttacat tgggtgtttt
     7141 ttcaataggt tcactctcac cagtaagact tgcctgactg acaaacaaat ccttcgcatc
     7201 caaaatacga acatccgccg gaatcatatc tccagcagaa agatgcacaa tatctcccac
     7261 caccaaatca tccataggaa gttctttttt ttcatagttt ctacgagtaa ctgtacaagt
     7321 ggtcgtaatc atggctaaca atttctctgc tgcatttccg cttctggatt cctgtacaaa
     7381 acgaagcgta ccggaaatga ataccattgt taaaatgatt acaacagtca gggaatcaaa
     7441 gtcctctggc acactgccaa acagagaaaa atacggaaaa atcatatctg taattgtgga
     7501 tactaaagcc agacaaaata aaatggcggt aaatggattg ataaatgcgc caaccagtcg
     7561 ttttgccaga gatttctttt tttcatgagt gactttattg gtaccatatt tagcacgact
     7621 tacggatact tcatctactt ctaagccacg aagtgtggta tgaagttttt ttaaaacttc
     7681 tttaatagga ttggttgccg caaattgaat gcggcggttc tgttcgtcac ggatgactgc
     7741 tttttctaca gtctgacgaa tagccatacg attttctttt ttgttcattg gtcttacctc
     7801 cttgaatttt cataggggca aggaatatta tatgagcgac acggaatctc caaattctca
     7861 tataatattc tctgccttcg tggactgaca tcagagtcca tgtctttcac ctcacaatct
     7921 taaaaaaata tctatgtgaa acctcaatgt ggattattgg ttgcgatagc ggacactttt
     7981 ttcgatatac cagacatcaa agccggcaac aatcagccat gcgattattg caaatacaaa
     8041 ccagtggttt gaaaatcccg gcaaaaatcc tttcattaag aatataatcc catttaacgc
     8101 aatcatttct ccaacattac gacaaagcgg gacaatatta attttatctt tctcctcctg
     8161 cggcatattt ttccacaggg aaagatgacg atatcctttt ccaaaggcca accaaaagcc
     8221 tgcaagagta aataaaattc caaaaaagat acatgtaaaa tccatcgttt gttcctcctt
     8281 tcaatgctta aacctgtttc ttagttttgt cagcttcaga agtttaatga agagcacaat
     8341 aatagtaaca gcaagaataa accagatcac aatactcacc tgataccata tcttaaagat
     8401 taccattctt tgttcaaaga aattccaacc caaatagcta cataagattg taaatacagg
     8461 gtagaaagca aaaagaattg ctcgtttcaa atcttcgatt tctctcctca ctcacaacac
     8521 ctcccaacca gcactaatca cttccggaat taccaatacc tgcccaacga ttccttctaa
     8581 aatattgttt ttggattttc cgacagaaca gtattctgcg acaatctcaa ccttgtcacc
     8641 aaccacatca ccgctttcca gattgttcaa aaagagcgtc ttacaggaat tgctgttaat
     8701 cagcaataag cgtatatcct gctctgcctt ttcctgacat gtaaccgaaa tacgatactg
     8761 cttttcagat tcatcactgg aagtaattgg gtgaaacttt cttgcaaagg gacgtagaat
     8821 caggttggaa ccaattaaaa tagtagcggc ggtgatcgcc attgcataca ttcccgtact
     8881 tgcaagaatt ccaatcgctg cagtacacca taaggtggca gcggtattca ttccacgtac
     8941 tgtccctctg tccttgaaaa taacaccact gcataaaaaa ccaacaccgg atataatact
     9001 gcttccgaca cggaatacct gatccgaacc gtagagcata ggaaacaagg taaagaaact
     9061 tgtacccatg cagattaaca cattgatacg aatacctgca ggatgaccag tcaattgccg
     9121 ttctaatcca ataagaaatc caagtgttaa ggagagggca attctcaaaa taaaatctgt
     9181 gtaaaacata tgaaacacct ccctttaact actaacagta tataaaaaat ccaaatacaa
     9241 tcctatggct aaagtcttgc gattttcttg cgatttggta agaaaataga cggactgaaa
     9301 ccgaaaatgg tcttagtccg tctttgtcat atctgatcat taaatttgta tccaataccc
     9361 cagatggtca aaatatatgt gggacaatcc ggagcaggtt caatcttttt tcgcaacttc
     9421 cggataaaag ccataatgtt gctgttatcc agaatataat cttcctccca tacggcacga
     9481 taaatctgtt cctttgtaaa aacttcacct ttgttctgga caagaaagta caaaatatca
     9541 aattcctttg gagtcaggtt gacttcagaa ccttttatca gtacttttct gcctcttgga
     9601 taaatttcca gatcccctgc caggattcta tctgacagag tacccactgg ccaatcgctt
     9661 tcttctgatg ctcctaacaa taacgaagat acctctccac gaatcatctc ctgaatacca
     9721 gaaagtaaat ccttcgcaca atcactgtcc ggtggatctc ttagtagttt ttctaatagc
     9781 cagatttcaa gagccgaatc catcggaata taactaatat tctttttcat catgaatgcc
     9841 tcctcttaaa tcaactcttg atattttttc ttataaaaat atttggctac tgaattcagc
     9901 agcatataga gagataccac cagcagtaaa aatacgaaat accaaagagg taatttcgtc
     9961 agtccaaaca acgacgcacc gccgacaaat gtaagggaag taaaagcaat aatccccact
    10021 agagttatag aaatgaccgg tgcagatgcc ctgctctgga aaaaaggaat cttggaagtt
    10081 cgcagaaaat gtaaaatcaa aacctgtgtc cacatagatt ccaaaaacca tccagtctga
    10141 aacagtgcta catactgaag ttgcagggaa ggatctgtaa gctgcagata ggttgcccct
    10201 ccacatagca tcggacatag gaaataatac aaaaatagga aggtgacaat atcaaacaga
    10261 gaactgatcg gtccgaaaga cagcataaac tgtctcagtg ttttgccgga ccagtctctt
    10321 ggggacagca tctcttcctc atctacattg tcccaaggaa gcacaataca caacgtatca
    10381 tacagcagat tcaaaagcag aatctgaatt gaagtcatag gcaggaacgg taaaaaggca
    10441 ctggcacaaa tgatggaaaa aatatttcca aagttagagc tagcggtgat tttaatatat
    10501 ttcagcatat tggtaaaagt ttttcgcccc tccattacac cttgctccaa aacattcaag
    10561 tccttttgca gcaaaaccac atcggcagca tcttttgctg cgtccactgc cgtatctaca
    10621 gagattccca catttgcttc attcagtgcc ggaatgtcgt tgactccatc accaagaaat
    10681 cccacggaat gaccattatc ctgcatagca gagacaagac gcaccttatg tcccggagta
    10741 agctctgcaa aaacatgagt atgttccaca gcctttttaa gttcacagtc tgtcatttca
    10801 tccagttgag taccagttaa gatacgctct gtataaatac ccacccggcg gcaaacagac
    10861 acagcaatcg ctgcctggtc ccctgtcaga atcttaggga taacttttaa tcttttgagt
    10921 gcagttacag actcctttgc tgtctgcttt ggtgcatcaa agaaggatag atagccaact
    10981 agagtcatgc cccattcgtc atcaggagtt attttctttt gcgctcccac attttttcgt
    11041 gccaccgcaa tgaccttcat tccgtcctgt aacatttcac ctactacaga agatacactc
    11101 tgtctcgcat cctgccccat ggggagaagt tttccacgat attctacatg gttgcagcgt
    11161 gagaatatat gttcaatatc acctttcata ataagctgac tattcccatt gctatcctgt
    11221 acaagtgtac tgacaaactt acgagcatag tcaaagggaa tttcatcagc tttttggaat
    11281 tttgtcagaa gatttgcgta atgggcttcc cgccctggca tggtattgca ggcaagaatg
    11341 gcattatcaa tggtattgca gacaccagaa tgatagcagc tattcagata agcaagatca
    11401 agaacctctg cattttcatt tccaaaaata tccatataat attccagcag gatgctttca
    11461 ttggtaagtg ttcctgtttt atccatacaa agcacatcca tgctaccaaa gctctgcatg
    11521 gcattaatat ccttgataat ggtctgcttt ttgctcatag caagacttcc tttggcaagg
    11581 caggctgtaa taaccatagg aagcatttcc ggcatcagac ctacagcaac tgacagggcg
    11641 aaagcaaaag attccatcca ttttccatca gtaatcccca gtaatataaa tacaatggga
    11701 actaatatcg ccataaaacg tatcatcacc caggcgatgg aatcggctcc ttttttaaag
    11761 gaatttttat catcagaatc cggtttggta aatcctccat acagggtatc tttgcctact
    11821 gccagtacaa tgccttcccc ttttccactg atgactgtgg ttgccataaa agcgaggttt
    11881 tcaagctgag tcagtgtatc ctgctctccg taactgagtt ttcggcagct cttttcaatg
    11941 atggcgctct cgccagtaat cgcagcctga gagataaaga gatccgtaat cttcgtcaat
    12001 ctgatatccg ccggaacacg atcacctgca gagaatagta caatatcccc taccactaat
    12061 tcttcgccag gaatctctat taactttcca tcacgcctca ctgtgacgct ttcatgaact
    12121 aggcgatcaa gctgagcagc agcgctcttt gctcgcagct cctgaatcag acgaattacg
    12181 ccactaatta cgatcatgga aaagatgata atggcggtag tagcatttct cgcaaaattg
    12241 gatgctacga aaacatccgt aacaagagaa acaatgccca gaacaaaaag aatcacatga
    12301 aacgggttaa taaatgcccg tctcaaccgt tgcatcgtcg tatcattttt ccgcccattg
    12361 aagctgttcg ctccgtattt ctcccgcatt gaaccgattt gctcaatgga taaaccatcc
    12421 ggagatgagc caatgtctcg ataaatttct gatgttctac agtatgcgta ttttttgata
    12481 cgactatcaa atagtgttgc ttttgacacg aaacatcccc tcctttataa gtataagtaa
    12541 taatatttta acacagtgtt ctgaaaatgc tatatttata ttcttgcttt tttcttgcaa
    12601 tttactttga tatggcattt ttcagctcga tcaaatccgt ctcgttaggg cgagataaat
    12661 ccttatcaaa gttttcaacc attccttcta aatttggagc acggatggat acggcaagca
    12721 ttttctcata gcgctctcgc acataaatag gcatttcccc ttgacacata tgagtgctga
    12781 caaacttctt cttggttttc accttgacca tgtatcaaat ttaaatgaac tacttagaaa
    12841 agttgctcat aataatattt gtattatgag tgatagaatg acaaaagtgg cagtatggaa
    12901 accgagtgta aaaacaatac aattagtttg tatggaatat ctgaactaca taattgacca
    12961 ctctgtaatt tatgaaaaaa tacaatgcgc aaattggaac gggacagaag aaaccaatag
    13021 tatttttaat aattcccctt caccattgat tactcttatc tattcttgcg atttacaaca
    13081 aaataaaaca acataaattt taaatacgtt gactggcata attcatatgt atagtaagtc
    13141 tacaattaag atgcgctttt tctaatccaa atatattaag aaaaaaattt tgcgaagcca
    13201 ttaatacttt gtttaagatg tctttcataa atataaagaa taatgctctt aacaaaagcg
    13261 tgcaaattcc taaagaaatg cacgctttca ataattatcc tataatcttc caattgctct
    13321 ccttatgtag cactaattca aactgcgata cctgtgttgc ctttgtctga ttatcaatga
    13381 atttcacggc aaccttgact ttcacattgt ccccatcaac cgtaaagata gggtttacca
    13441 attcagaata gaggtagtcc ctgccgatag actcaagcac atttccagac acatagtaag
    13501 caagctcctt ttctgtggct gttgggtata gcttaaagaa agtttccaga aatgcagtag
    13561 cgtcatttat ggtatccgcg tctacacttg cgtctgcttc tggtgtcttt ggttcatagt
    13621 ctgatttctc gattgctggt gcaagggtag ggttctgaat aattaccata ttcccgccag
    13681 cgtccacatg aacttttacg gtataggttg ccttaaccgc tgttgtctgt tccccctctt
    13741 ttatctgctg atctacttcg taggtagcgg aaaaagtgtc cgttccagac tgttcaatgc
    13801 tccatacaat cacatctgta atggcagagc tggtcggtat atccgttcta atggtatcca
    13861 tattcaagtc ctgcaattcc tttgtcagat aaccgctgat tgcctgcgtc cttgcgtcaa
    13921 tcgcttcttt gctgttactc catgtgtaat aggactttgc aaaattcttc acgaaatttt
    13981 ctatcccgtt ggtgtcctgc aagcgaagct caatcacttc tttttcatgg gtcgtgtgct
    14041 ggtcaatagc ggtaaaattc ttatatactc caaagctcac gcttgcaata agcaccaccc
    14101 acaatgcaat cacggttttc ttatgtgtgc ctaccttgac agtacgtacc tttttttctt
    14161 tgacattttc tgtctgtttc ttattcttct taaacatatt tttcaagtcc tttctattgt
    14221 tttactcgtc ctgcaccgat taagtgctgt tgccagtaac tactacttag gtctgtatat
    14281 cctatcgggt cgcctgcatg gtacatctga ttatttccca catagatacc gacgtgggtt
    14341 acatacgaac cagcgttata ggtggaatgg aaaaatacca aatcgccagc ctttgcctgc
    14401 gagagtggaa gatgttgggt agcgtcatac tgggcttgtg ccgttctcgg taaggagata
    14461 ccagcttttc catagcacca ttgcgtcagt cccgaacagt caaaggaagt ggttggagaa
    14521 gaaccaccaa acacatattt ccacccttga tatttcaacg cttcattcat taccttttgt
    14581 gccagctctc ccgatacctg cggaacagtt aaatactgat tgactaattc cacatagaac
    14641 atatttccat agccataccg ccagcctccg ttcttcgcaa cggctatcgg gttggtatag
    14701 gttactttct ttccacccga tttctcacgg gcgaagctct ctgcaagatt aaaagtgtgt
    14761 ttctttccgt ttcctgccac atagcccaca tagccaccgc catagttata ggactgtatc
    14821 gctacattca aatcgtcgat atcttgattt ttgcaggaag aaagcaggga cgcaaaatac
    14881 ttacacccct gcttgattga gctttccgta tctaaggaac taggcggtaa accaagactt
    14941 tctgaactct gcataacatc ttctgccgta ccgccacttt ctacttgaat gatagccagt
    15001 agtacattga cgtactcgga gataccgttt tctctggcgt atttttctac cataggctga
    15061 tgtttcaaaa cttctgcgga taagttcata cccgtaatgc cagaagaaaa gttgctgttt
    15121 tcgtcgtcgc tgtccgcact aatcagcacc ccaaagaaaa gcaccagaga aaagagaata
    15181 ggaaacagac ttccaatgat agcgatatgt ttcagtttca ttttttcttc tgtcctttct
    15241 tcgttacaag attacggatt ttctctgtgg tctgctggtt cttctggaca gtctgggtct
    15301 gctgaacctt tgtcctacgg tctttggtgt aattctggcg tgtttccttt gacacggtaa
    15361 ctttttctac attctgccta tatgtcgatt gggtcgcttt cccgtcagga gtactagaag
    15421 atagcggacg ttctttgaca ccgcttgtct taactggttc gcttgctttc gtagtcggag
    15481 ttgtggcagg gcgtttgact tcctgcattt tttccgcacc cgtctttgaa acggttgaag
    15541 ctgtggctgg tcgctcatgg ggacgggtag ctcccgttgt cgctgatccg tcagcctttc
    15601 gctgtgcctg ccttgcttct tgtgcctttt gaagctccat acgcttgaca gcgatacttt
    15661 tcttatgctg ttcctgcttt tccaagcgtc ccgtctgtct ggactgctgt tcctgcacca
    15721 taccacgctt gaaatcagac acgctggact ttgccttttc ctttgcggaa tacaccgcat
    15781 aagcggtctg tgtgggcata tccttgatat tctctttgac agcgtttgcc ttgtctttca
    15841 ctttattttt cgtatctaag acagcaccca cctttgagcc tgcacgctgt cccatactgg
    15901 aagtggtatt gccccgattt tctttagaag ccgtattttt tctttcggat ctcttgccag
    15961 caacggcact tccagctacc gcaccagcca caccgcccga aataccgcca gcacttacag
    16021 cccttgcaat acggtgttcc atacgcctag cccgatgtcg catgaacaga tacggtctgc
    16081 ggaatatcct gcgtcccatg ctttgactgt cgccagcgtt gagagaaaac atactcatta
    16141 agtcgcccag cttcatatag ataccagcga aacatactat ctgcaagaac gccaccatga
    16201 aaaacggata gtccgtggat atgttataaa acatactgga aatgctaaac gcaaccgtta
    16261 caatgagcgt tattcctgcc cgtgtcatta tggtattaaa taccctcacg attgcctgct
    16321 ttgccatgct ttcatagctc ggtatcatgg aaagtagaaa gctgataggt aaaaacatag
    16381 cgaagataat aaaaagtatc tggctgaaca acatcatacc cgtaagcaag aatacaaata
    16441 tcgtaatacc aagattaaag aacaagagga agaacaccat acctaaacgg tttaccacct
    16501 gcggtatcgt cagattgttg ttgtcgttgt cctcgatttc tgttttcacg acttcttctc
    16561 tggtctttcc gtcctcgtcc tctggacttg ccgatacgag agcttcgaca cggtctgtcc
    16621 cgatttcctc tgcgttgctg tttccaaact gtaagagtag ccacggctgt tgtacttgaa
    16681 tagaaaatag gctgtcccgt atcaagtcta cgctgtcctt gccctcgctg tcagagttgg
    16741 ggagcatgat ttttgttccc aaatcaagcg aagcggtact gatgtctgat gaaaattcat
    16801 ttatcttctt gatgtagtcg ggagcgtagg caataaacga agcggacaac acgaacacca
    16861 ccacaaagtt gataacagcg tgaagtgcct tgctggtttc ccgttttatc agtcccgtat
    16921 aggcaacata cattcccacc actaagataa taagaagcag gaaaccaaca tagaagcccg
    16981 tagaagaaaa gccgttctcg gtaacgcctg caagggtctg tatgcttttt ccgatactgt
    17041 ctgccatatc gttaataaaa tcaagtttat aggcttcctg caccacatag ccagttgcat
    17101 tgcttaaata aaggcttatc gtccagacaa agttggtaat gcagtaaagc ccgtactgta
    17161 ccgattttcc gataccgtcc agccagttcc acggaagcca cgaccagcta ttatcgacga
    17221 aaaaatcaag ctggtagttg gaaagcggat attttgaata tagattttct gcgtttatgg
    17281 tatcgtccac aagccccgtc gcatgagcca ccgtccccag aagtgaaagc aggataaggg
    17341 aaagtgccac cacgaacaga gccattttga gaaagtgaag tatcttcctt tttgtgaacg
    17401 cactctgtat cctttctttc atcactccac ctcatttctc tgtacgggcg gtctagtatc
    17461 aaaggcgtgt agtaattctt caaagactgg gtgtatctgc accacaccga cacgcccgta
    17521 taagtcctgc aataaacatt gtccgttctc caaatcacgc aagcgtttct ggttgttttc
    17581 gtcgtccttg tcgataccga aaaattctaa ggtctgcttt atctcgttga tgtcagtaga
    17641 acgaaatgca aacttcaaac cgatattgtt tttcagactt tcctttgaca cgtcataggc
    17701 agactgggta acgaaataaa cgcctgcctg catagctcgt ccagcacgaa ccagcttatt
    17761 tgatagggtt tcgccttgtg ccacatttaa gaacgcccac gcttcgtcaa ggtctacaat
    17821 cttaaaaata cttctgtccg aatggataaa atcaagggca aaggtactaa tcacaatcag
    17881 catagatacc gacaataatt caatggtcgt gtattcctca aaggttgtgt ccttatctgg
    17941 cagtacaagg tcagctactt gaatgatatt gagctggtta tccagactga tagcattttc
    18001 taccgtaccg tctgaaaaca gcaggtgtgc aaaatcatag tccgtgaagc tgtcgatatg
    18061 gtctgcgata ttccttgata ctggcgtatc ttcacggcgt agctcgtcta ttacatggag
    18121 caagccccga ctgtcgctct gggtaacgga acgaaccgct tttcgcaaca cggggaattt
    18181 ttcgccgtcc ctagaggaaa taccagtaag gaatgtcaag atgtcgattg ccagactttc
    18241 agcgtctttt acattcttca taatcacaaa cgggtcaaga agccctgcat tgtccttgtc
    18301 gctggtaagg tttacgatat tgatttcatg ggcgatttct gggagcgttt ctttccagtt
    18361 gccacgctct gatttagggt ctaagataac tgcttgtccc ccaaagagaa ccgaataata
    18421 caccagaaga ttgttacaga atgactttcc accgccaagc gaaccgacaa aagcagaagc
    18481 taaagcgttg gtaactgtac cttttatacc ttgactggca agggacggtt gcaggtacac
    18541 atttcttccc gtatcaacgg aatagcccat atagataccc gtagtttccc ctaactgctg
    18601 tgtcgcacca aagccaagcc cagccaagaa gtctgatttt acatactgca cataatcgtt
    18661 gatatatcgc ttgctggcag gaa
//
LOCUS       DACXMZ010000061        18508 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.061, whole genome shotgun sequence.
ACCESSION   DACXMZ010000061 DACXMZ010000000
VERSION     DACXMZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.061"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            126..1319
                     /locus_tag="JD016_08275"
     CDS             126..1319
                     /locus_tag="JD016_08275"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAU4999634.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1525..2643
                     /locus_tag="JD016_08280"
     CDS             1525..2643
                     /locus_tag="JD016_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HAU4999635.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAIN"
     gene            2765..3019
                     /locus_tag="JD016_08285"
     CDS             2765..3019
                     /locus_tag="JD016_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HAU4999636.1"
                     /translation="MIFLKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVG
                     ERGWAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3006..3752
                     /locus_tag="JD016_08290"
     CDS             3006..3752
                     /locus_tag="JD016_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAU4999637.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKSEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4037..4912
                     /locus_tag="JD016_08295"
     CDS             4037..4912
                     /locus_tag="JD016_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAU4999638.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIITTSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5303..6103
                     /locus_tag="JD016_08300"
     CDS             5303..6103
                     /locus_tag="JD016_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HAU4999639.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            6631..8871
                     /locus_tag="JD016_08305"
     CDS             6631..8871
                     /locus_tag="JD016_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAU4999640.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYKSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            9285..10820
                     /locus_tag="JD016_08310"
     CDS             9285..10820
                     /locus_tag="JD016_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAU4999641.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVIIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQLAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            10836..11597
                     /locus_tag="JD016_08315"
     CDS             10836..11597
                     /locus_tag="JD016_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4999642.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            11935..12804
                     /locus_tag="JD016_08320"
     CDS             11935..12804
                     /locus_tag="JD016_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAU4999643.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            13133..13384
                     /locus_tag="JD016_08325"
     CDS             13133..13384
                     /locus_tag="JD016_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAU4999644.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            13496..14275
                     /locus_tag="JD016_08330"
     CDS             13496..14275
                     /locus_tag="JD016_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAU4999645.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLEFENDVDEEMNWIITRAN
                     ELIKKYPSQKEMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(14410..15060)
                     /locus_tag="JD016_08335"
     CDS             complement(14410..15060)
                     /locus_tag="JD016_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAU4999646.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(15057..15560)
                     /locus_tag="JD016_08340"
     CDS             complement(15057..15560)
                     /locus_tag="JD016_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAU4999647.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(15609..16124)
                     /locus_tag="JD016_08345"
     CDS             complement(15609..16124)
                     /locus_tag="JD016_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAU4999648.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGNKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            16470..16931
                     /locus_tag="JD016_08350"
     CDS             16470..16931
                     /locus_tag="JD016_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAU4999649.1"
                     /translation="MRELLKWVSIINRYNTNYIDRKLADIGINNSQYFYVLHICNNPG
                     ITQDTIFEKILVNPSNITRALANLEKEGFITREPGVKDKRTWHLYPTEKSLGCYDEIL
                     KITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMNEEAKLEAKENGRKI"
     gene            16939..18318
                     /locus_tag="JD016_08355"
     CDS             16939..18318
                     /locus_tag="JD016_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAU4999650.1"
                     /translation="MEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIGQGVGVLGN
                     AATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICLMAVFGISL
                     FIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLILADGRPKAS
                     MFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKNFQHIKLHK
                     KSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSEIPLACVGI
                     IIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIISVIAFLCFQ
                     FFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGKSVLGLFTS
                     LTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSLTIKQNNLM
                     SNHNKIAEV"
BASE COUNT         7090 a         1962 c         3347 g         6109 t
ORIGIN      
        1 aaatggaaat aaaaaataaa ttttatgaga atttaataac taataattaa tatatttagg
       61 ttataataaa tatgtgcgta aaatctaaca ataagcaata ataccgtaaa tttaggagga
      121 ataatatgga tactttaaag gaacttttta aaataggaag tggaccttca agttctcata
      181 ctatgggacc acaaagagca gcagagagat ttaagaatga aaacccagat gcagaaagtt
      241 ttagagcaat attatatggt agtttggcag ccacagggaa aggtcattta actgactata
      301 taattgaaaa aactatagca cctaaaaaag tcgaaattgt ttgggaagaa gatattataa
      361 aagattttca cccaaatggg atgaagtttg aagctttaga taaagataaa aatgtaacag
      421 ctgaatggac tgtatattct gtaggtggag ggactatagc agaagaaggt caaagaaaca
      481 gtaaaagcaa tagtatatat cacttagata ctatggatga gatagtaaaa tggtgcaaag
      541 aaaataataa gactttggta gattttgtgt tggaatgtga accaaaagac ataaaagatt
      601 atataaaaac tataaaagat gctatgagaa aatctattga tgatggacta agtacagatg
      661 aaataatacc aggcaagtta ttattaaaaa gaagagcaag tacattttat aatgcatata
      721 aaaaagataa gagcttctca acacttgtat atgcatatgc actagcagct tcagagcaaa
      781 acgcttctgg taatataata gttacagcac caacttgtgg ttctgctgga gtaataccag
      841 gaatattctt tgcaatgcag gatttctata attatgatga cgaaaaaata attgaagcac
      901 tacttgtagc tggaataata ggtaatataa taaaaactaa cgcttcaata tctggagcag
      961 aggtagggtg tcaaggtgaa gttggagccg cttgttctat ggcagcagca gcagtagcat
     1021 acttaaaagg tggaactata gaccatattg aatatgcggc agaaatagca cttgaacatc
     1081 atttaggaat gacttgtgac cctgtatatg gatatgttca aattccatgt atagaaagaa
     1141 atgcaatggc agctcaaaga gcttatgatg cagctaatta tgcattatta actgatggct
     1201 cacattctgt ctccttagac caagttgttg aaacaatgaa agaaactggt atagatatga
     1261 tggataagta caaagaaaca gctaagggtg gacttgctaa acatttcttc tcttgctaaa
     1321 aataaaattt tccaataata attcctctgt tttgatttat gaaacagggg aattattatt
     1381 aatggtgaat tatttaataa tgcaaaatat ttttatattc aatttagctt tttattttta
     1441 attagtataa catgctatat aatattttaa ttataaagaa atatagaaat attttttaat
     1501 aaattagaaa ttggaggaga ttacatgaga gaggcatcat atttacaaga acaactttat
     1561 aaacatagga agtcattaaa tcctattgca gagattggga gaaaagagta taagacttca
     1621 aaatatataa gagattatct tgataaatta ggaattgagt atgaagttta tttagaaaca
     1681 ggtatagtag gtgtaataga aggtaaaaga cctaaaaaag atattgcttt tagagctgac
     1741 atagatggtc ttcatagtga agaaggggta aaacatttat gtggtcatga tggacatgct
     1801 actatattac ttggattaat tgagtattta aatgataata aagaagactt aaatgataat
     1861 atagtattta tattccaacc agcagaagaa ggtcctgggg gagcaaaagc tttaatagat
     1921 gaaggagtgc ttagaaaata caatattgat gaaatatatg ggcttcatat atatccagag
     1981 ttaccagaag gttatgtagg tacaagagag ggctatttta tggctcaaat aggagatatt
     2041 gacatagaaa tagtgtctaa aagtggacat ggggctatgc cacaaaatgg tattgatggt
     2101 atagtgatag cttcaaactt tgtaagctca cttcaaacta tagtgtctag aaatataagc
     2161 ccaatagaca atgcagtgtt gactattggt agaattgaag ggggagctag aagaaatata
     2221 atagctgaaa atataaagat ggaaggtact atgagatgtt tcagcccaga agtgtatgag
     2281 aaaatgaagc ttagagttag agaacttgct agagggtttg aattggctta taactgtaag
     2341 gttaatataa atattataga tgattatatt gctgttaaaa atgacaagga tttgtatcaa
     2401 gagtttgtag aggctattgg aagtgatact gttgtagagt tagagccact tatgatatca
     2461 gaagattttt cttattatca aaaagaagtt ccaggattat tttttatgtt gggttcaaga
     2521 aacgaagaaa aaggatttgt aaatggtctt cataatataa actttaattt tgatgaaaag
     2581 atatgtgtaa atgcattgaa tgtttattca aaattattaa aatacaaaga agcaataaat
     2641 taggaagata aattataaag attaaaaagc ataaatacct aaatttaaaa aaagtgtatt
     2701 aataaatacc ccttacatat ttgtataaat ttattttaat tgacaaaaat acaaataaga
     2761 aggggtgatt tttttgaaag aaaaagtaca aagagaaaag aagataacta atggcaagaa
     2821 tactaaaata ttgacaaatg atgatataat gaaatatgag atagcctcag aactaggtct
     2881 tatggataaa gttggtgaac gtggatgggc aggacttaca gctaaggaag caggaagaat
     2941 tggaggtatg cttacatcac gcaaaaagag gcttaaaaag attgtggagg agcagaataa
     3001 aaaggatgag tcaatatagt gattttgcgt ttgtatatga tgaacttatg aatgaagttg
     3061 attataatgg ttgggttaaa tatatagaag atataataga tagtgaaggt gtaaaagtaa
     3121 aaaatatatt ggaattggca tgtggcacgg gtaatcttac aattccattg actaaaaaaa
     3181 attacgatat agctggtata gatatatctg atgagatgct tagtgtagca agagaaaagt
     3241 cagaaaaaga aggagtagaa ttagttttac ttcaacaaga tatatcagag cttgattttg
     3301 aaatatctga tttggactgt gtgctttgtg cttgtgatgg atttaattat ataacttatg
     3361 atgatgattt ggaaaatgta ttttcaaaga catatgaatt attaaagaaa gatggaatat
     3421 tcatatttga tataagttct ttttataaat tagccaacat attgggaaat aacatgtatg
     3481 gagaaaatag agaagatata gcgtatatgt ggcaaaatta ctttgatgaa gaagagaatc
     3541 taattgaaat ggaacttaca ttctttatta aagatgaatg tggaaagttt gaaagatttg
     3601 aagagataca ccaacaaaga gcatatactg aagatgagat agttgatatg cttgaaaaat
     3661 ctggatttaa ggatataaaa tattatgggg attttacatt tgacaaacct agtgaagaaa
     3721 gtcaaagaat cttctttgta tgtaaaaaat aagatattct tgactatata agataattat
     3781 taaaaataac catgatataa aaatatataa aatgcagttt tatagattaa aagtaaagtg
     3841 atgatatttt atgattttta ttttctaata attataaagt tttatagata aaaattaaaa
     3901 tataataact attgcttagt aaaattaaaa taaaatgggt tttgcccaag gttaagatag
     3961 ataagtattt atagattaaa attaaggtag taatataata aaaataagac aattaacggg
     4021 acatggagga atattaatga gagattatgt actaagagca acatcaggaa atggtcaagt
     4081 tagagcattt gtcgcaacta ctagaaatac agtagaagaa gctagaagat tacatgaaac
     4141 tacaaaagta gcaacagcag cacttggaag aacattaaca gccacatcta taatgggatt
     4201 aatgatgaaa aatgatagtg ataaattgac tgtaataata aaaggtggag gtccaattgg
     4261 aactattatc accacatcag actctaaggg aatggtaaaa ggatatgtag gaaatccaca
     4321 agtagaggtt gaagactatc caaatggaaa gttaaatgta gcagcagcag taggtacaga
     4381 aggtgttgta aaagttataa aagacttagg tcttagagaa ccatataatg ggacttatcc
     4441 attggtaagt ggagaaattg cagaagactt tacttattat tttgctgtat cagagcagac
     4501 tccatcagta gtagctttag gtgttcttac aaaagaagat gaagtagagt ttgctggagg
     4561 atttatagtt caattaatgc cagatgcaga agaagagact attgctaagt tagaggaaaa
     4621 tgtagctaaa ttaccttcta taactaacat gttaaaagaa ggaaaatcac cagaggatat
     4681 attaaatatt gttttggatg gtttggaacc taaaatactt gatacctgtg aagttgggtt
     4741 tatgtgtgaa tgttcaaaag aaagagtaaa aactgctttg gttgcaattg gtaaaaaatc
     4801 attggctcaa ataatagaag aagataaaaa agcagaagtt ggttgccagt tctgtaataa
     4861 gaagtatatg tatagtgaag aagagttgct tgaaatttta aaggaaatgt agactataaa
     4921 aatagttgta ttgatataag taatgttata gtgatatagt aaagtcgtat tgatatagta
     4981 attaaagtat aatttattaa taatagacat atttatatta aaaaataagt ttaataaatt
     5041 tttaagctta ataaatttta agaattaaga agctttctgg ctgtaatgtt tgcaatgttg
     5101 cagttagaag gcttttttat tttattatag ttcgtgtcaa agattgtatg aaacaataaa
     5161 atattgtaaa ataatttgta tatttagacc tgattgacac aaaggtttat aagatatttg
     5221 agttgattat acgaaattct atgaagtagt aaccctgtga ctttaatcat gggaggttca
     5281 gaagaaggag aaaattttaa atatggaaag taaaacgaag tacataggaa taagtatatt
     5341 aatattattg atgatatttt taattgcaaa aaattttaac ggaaaacttg aaatacctat
     5401 tcctgagagt aatagtagaa aatttaaatc agcagcagtg ttttatcctc aacatcaaga
     5461 tgatgaggtt ttatggggag gaagtgccat tgtagatgct ataaaacagt gtggtgttga
     5521 taatgtatat gtggtattag tatcagatgg ttctggagtt aacgttttca aagctaacac
     5581 aaaatttaga aatttaacta ggaagcaaaa ggaagaactt agaaataacg agtttaaatc
     5641 agctttgaga gagcttggag tgaaacctca aaatgtgatt atacttgcag atatagataa
     5701 aaaggaaggc acacattatg aattaatgga aaagactata ttggattttg aacataagtt
     5761 taaaggcaat gtgacccata tagcccatca ctataagtat gatgaccata taatgcatag
     5821 aaagaatggg gaagttttaa agaaacttaa acgtgagggt aagataaaag atgacttgta
     5881 ttttgttaag ccaaaatatg taaagtatat accaagaaat gaaagagcta tctataaagc
     5941 aatggatttt gatgactaca ataaggttaa gagtgcttgt tatgaatata aggttgtaaa
     6001 ttcaaagcgt aaaatgtatg gtataggata tatttcggca catagttatt ttgaacatct
     6061 tctaaatgac cctgaactta cgtctgtatt aagtgaagat taatttctta taagtagtaa
     6121 gttatgtttg ataagttgat tggaattttc tatagttttg aaggtattgg aaatttagtt
     6181 ttatttaaag atttaaagat agaggttttt aatgtttaaa attttaaagt taagttgagt
     6241 aattgtatag aattatttgt gaaagaagaa acagtgtaaa acatagtatc ttatttatgg
     6301 ttttaaaatc ataggcaaga tacttttttt atgtaactaa taccattttg agattattaa
     6361 cttaagaatt ttatataatt taacactcat atataatgat gatatttagg tagaatagat
     6421 gtaattacgc ttaaataaaa taacggtatt ttttataaaa atccaaaccc tacactatac
     6481 taccagataa aatctacatg ctataatttg acctgtgttt ataaaaaaag gagtaatcta
     6541 ataaaatagt atttcactta tgtataacta acaactatta ttacgatttt ctctaaataa
     6601 tttaaagaaa aataatgcag gaggtaaaat atgaacaatg aatttataat tttaaaaggt
     6661 tgcaaagaga ataatttgaa caatatttcc ttaaaaatac caaaaagaaa aataaccata
     6721 ttcacagggg tgtcgggttc tggaaagtca tctattgtat ttgagacaat tgcaaaagaa
     6781 tcacaacgcc aactaaatga aaggtttagt acatttgtaa gaagtttttt acctaagtat
     6841 ggagaggtta aagctgattg tatagaaaat ttgtcaacac caattatcat agaccaaagt
     6901 agacttggtg gaaattcaag gtcaacccta ggtactatta cagatgtaaa ttccttttta
     6961 agagctttat attcaaggtt tggtagtgag tatataggta atgccaatat gttttctttt
     7021 aataatattg atggtatgtg cccacactgt catgggcttg gaaaaaagct agttcctaat
     7081 atgaaccaaa ttcttgatat gaataaatca ttaaatgaag gtgctatatt gctttcaggg
     7141 tttggagtgg gttcttggca ttggaatata ttcgctgaat caggattttt tgatattaat
     7201 aagaggatat gtgattattc agaagtagaa ttggaaaagt ttttgcatgg taaagcagaa
     7261 aagattaaga tagaaaatgc tggtcaaact aacatgacat atgagggatt aatggttaaa
     7321 tttaatcgcc tatacttaag tagagaggga gaaatatctg aagctactaa gaaaaaattg
     7381 agtaaacttc ttatagaaga tagatgtcct atatgtgatg gaagaagact taatgaaaga
     7441 gtatataagt ctttaattaa tggttataat atagcagatt taactagtat gcaaattgat
     7501 gaattggctg aagttattaa aagtatagat gagccagaag cacaaccttt aattaaagga
     7561 ataatagaaa agttaaacag tatcatagat ataggtcttg gatatttaac actggataga
     7621 gagacatctt ctctatcagg aggggaatct cagcgtataa agatggttaa gtatttaaat
     7681 agtaatcttg ttgatttaat gtatattttt gatgaaccaa gtgtaggact tcatccaaaa
     7741 gatgtgtata aattaaataa tcttttaaaa aaattgcgtg ataaagggaa tacaattata
     7801 gttgttgaac atgacccaga tgttattaag attgcagacc atattgtaga tgtaggacca
     7861 aaggcaggta agtatggtgg agaagttgtg tatgaaggca gttatgaaaa tctacttaca
     7921 agtggtactt tgacagggaa tgctttaagt aaatttcttc ctattaaaga aagtgtaaga
     7981 gaacatagtg gatatttaga agttaaaaat tgtaataaaa acaatttaaa aaatgtatcg
     8041 attaagatac ctaaaggtgt attgactcta gttactggag tagcaggagc aggtaaaagt
     8101 acgttgatta aggatgaatt tttaaagcaa aatcctagtg ctgtacttat agaccaaagc
     8161 cctgtttcag caaattcaag gtcgagtttg gcaacatata gtggaattat gaacaacata
     8221 agaagcatat tttcaaaaac aaatggcgta aatgcttctt tattcagttc aaattcagaa
     8281 ggagcttgtg ataactgtaa gggttctgga atagtagaaa tgaatcttgc ttttatggag
     8341 agtataaaga gtacctgcaa tgtttgtgaa ggtaaaaaat ttaaaaaaga agttttagaa
     8401 tataggtttc aagggaagaa tataattgaa gttcttgaaa tgtctgtttt agaggcaatt
     8461 gagtttttta atttaaaaca gattaaaacg aaattacaat gtattgaaaa aatgggtatt
     8521 ggatacctta ctttaggaca aactcttgat actttgtctg gaggagagtg tcaaagatta
     8581 aagcttgcta gtgaattaaa taaggaaagt tcagtgtata tattagatga accaacaaca
     8641 ggtcttcata tggctgatat tgagaatttt ataagtataa ttgaagagat tgtagataat
     8701 ggaaatacgg tcataataat tgaacacaat atagatgtaa ttaaaagagc tgattggatt
     8761 attgagctgg gacctgaagg aggaacaaaa ggcgggagag taatatttga aggtattcca
     8821 aaacaattat gtaacaataa aatatcatta acagcaaaat atatagtata ataaataaaa
     8881 tataataaaa ttataatgat attttaataa atataatggg tatataaaat gataattaaa
     8941 cttaacagta ataaataatg tgagaaatat tcaaatttaa ttaaataaag tgtcagaaat
     9001 ttgtttaaat tttagtattt taacggttct gacctttttt cttattttta tggagaatat
     9061 cgaggatgga gtctctaata gttacttttt tgtgtctact tgcaagtttg attaaaaaat
     9121 ggctatacac acttgtgact tcagtcatgg gaggttcaga ttataatctt aatatataat
     9181 ttgtgaaata atttgatgac gatttgctga tgtataaaca ataaagatta actacattaa
     9241 aatttaaatc ttgttaagta aaacttataa aaggagagag atggatgata aatgatattc
     9301 taaccttatt atctgaaaga agtgattttt ttataaagct atccatagaa catttgatga
     9361 tttctattat ttctataata attgcgattg ttttgggtgg tataattggt attattgtaa
     9421 gtgaatttca taattcttct aaacctatat tgggaactat caattttcta tacaccattc
     9481 catctatttc tatgctagga tttctgatac cattttctgg aattggaaat actactgctg
     9541 ttattgcact taccatttat gcattattgc caatggttag aaatacatat actggaatta
     9601 ctaatgtgga taaaaatatt cttgaagcag ctaggggaat gggaagtaca gattttcaga
     9661 tattgtataa aataaaactt ccgttagccc tacctgttat tatgtctggt attagaaata
     9721 tggtgattat gacaattgca ttagctggta tcgcttcatt tatcggtgct ggaggcctag
     9781 gtgtagccat atatagagga atcacgacaa ataacacaat aatgacaatt actggaagta
     9841 tacttattgc tctattagct ttaatatttg attttatttt agggctggta gaaaaatttt
     9901 ttcaacgaaa aagaaaaaag aaaaatagga aaaaagtttt tgccattcta ggaagcctta
     9961 ttcttatagt aattatcttt acgtcttatt tatttaatcc ttcaaagact caaactattc
    10021 atattgctac taaacctatg actgaacaat atattcttgg tgagatgttg gatattttaa
    10081 ttgaaaaaaa cacaggtcta aacgtggaaa ttacacaagg tgtaggaggg ggaacatcta
    10141 atatccagct agctatggag aatggagaat ttgacattta tccagagtat acaggtacag
    10201 gctggaatat ggtacttaag aaaaatgaat tatatactga aagtatgttt agtaaactac
    10261 agtcggaata caaaactaaa cttaaaatgc aatggactgg tatgtatggt ttttctaata
    10321 gctatgcatt agcagttaga aaagaagttg cagataaata caatttaaaa aatataacgg
    10381 acttaaaaga tgtatcttct aaattgacat ttggagctga atatgacttt tatgagagag
    10441 aggatggata tactccattg tgtaatacat atggactgaa ctttaaagat acagttgatt
    10501 tagatattgg attaaaatac caagctatca atgaaggtaa gattgacgta atgcctatat
    10561 ttacaactga tggtcagtat agtgtttcag atgttgtagt tctaaaagat gataaaaatt
    10621 tctacccatc ttatcaatgt ggaaatgttg ttcgtttgga agtattagag gaaaatccag
    10681 aactatcaaa ggtatttaag atggttggag atattttgac tgataaagaa atggctaaaa
    10741 tgaattatga cgtagaaaac aacaatttag aacctagaga agttgctcgt gagtttctga
    10801 ataagaaagg tcttttataa attggaggaa atataatgac acctattata caatttaaaa
    10861 atattaaaaa acaatataat gataaaacta taatagataa tcttaattta gatatagaaa
    10921 aaggagagtt tttgactgta attggttcgt ctggttctgg taagacaaca ctcttaaaaa
    10981 tgattaatgg tctgattttg ccagatggag gaaatatttt gataaaccaa acagatatca
    11041 aaaacgagga cttaattaaa ttaagaagaa gaataggata ttgtgtacag ggaagtgttc
    11101 tttttcctca tatgactgtt gaagaaaata tttcatatgt accaaatctc ctaagtaaaa
    11161 agaataaatt agaagtaaaa agtgctgtaa acaagtggat ggaaatagtg ggattgccaa
    11221 atgatatgaa agtgcgttac ccatcagaat tgtcaggtgg tcagcaacaa cgtgtaggga
    11281 ttgcaagagc tttagcttct tctccagaaa ttttattaat ggatgaacct tttggtgcag
    11341 ttgatgagat tacacgaaag caattacaaa aagagataaa agaaattcat aaaaaaacag
    11401 gagttacgat tatctttatt acgcatgata tttatgaagc tttgattttg ggaacaaaga
    11461 ctttggtatt aaatcatgga gttgttcaac agtatgatac tcctgagaat attttaaata
    11521 ctcctgctaa ccaatttgta gaccagttat tgaatataag aaaatctata atagatgatg
    11581 ataatttaaa agaataaatt taaatttaaa atcaaattgt tgtgataaaa actattgatt
    11641 tagagctaac ttcaagttgt atacttgaag tggaaaagtt agattaaggt atagaagggc
    11701 atttagcagt tggctgtaga cgaggtaact gctgaaggtg ctttcaataa atggctagaa
    11761 cctgtatctg ttgaagatta taataaattg aaataagaag ctcaatatac agagtagata
    11821 ggcacttcaa atagaagtgt cttttgtgtg ccataaaata aatcaaaata ttagcaaaga
    11881 atgtgaaaaa caaaataaaa taatatgcta tctttaaaat aggagggatg ctatatggat
    11941 aacaatttga ttaccattga aaaaacttta gattatatag aaaaacatct tttagatgag
    12001 atgttagatt taaatgtaat ttctaaagag ataggctatt caaaatacca tttacataga
    12061 atgtttacat cagttgtcgg atttagtgta cataattata tacaaagacg tagacttact
    12121 gaagcagcga gaatgctaat ttttacagaa aaaccaatta ttgagattgc tttaatctcg
    12181 gggtatgata cacaacgctc tttttcaaga gggtttaaaa aaatgtttaa acgtagtcct
    12241 aattttttta gaagacatca agatttttta ccactacaat taaaatttga tattcataat
    12301 agaaaaaaat tacgtggaga tatggtttta gatgttaaaa aagttaattg tgcagatata
    12361 aatattgttg gctattctgg taaagtaaga aaaggatttc atattattgg aaaatgctgg
    12421 agggatttgc ataaaaataa agagatgata gaaaataagg tagatttagg ttttttaatt
    12481 ggagttagtg attatagcaa ttttaaagag attgagaata ctcctacatt tacatacatt
    12541 gcaggtgctc aagtagctac ttttggcaaa ttacctaatg gaatggaaaa attcgtatta
    12601 ccgaaaagta attatattgt ttttactttt attgggaaaa atgaggatag tatgcaaaat
    12661 gttgtagaat atatctatca agaatggttc cctaattcaa gttgccagtt taatgaaaat
    12721 aatttatatg actttgtaaa atatggagaa aaaactgatg agaatggttt gagtgaaatt
    12781 cagttttggg ttccaatcat ttaaaagaag gtattatgta taaagtagat ggatttatat
    12841 aggcaaagtt ttacagcagt atgtatttat aaataattta tagtttgtag gaaagaaata
    12901 attttactat aagattatga taaaatacac aatactatga caaaatagtg ttaaataata
    12961 gatttaattt tgcataggaa aagttctata atggtgtgta ttcataaata atttataatc
    13021 tgtaggaaag aaatagtttt actataagat tatgataaaa tacacaatac tatgataaaa
    13081 tagtactaaa taatagattt aattttgcaa aggataaggt gttttaataa aaatgaatgt
    13141 agaaaacgaa ttacttaaaa acctagatag attgcataca acagagttag gcgttgtaag
    13201 aattaagaaa aatctttctt tggagacaaa tgatgtagtg aactggtgta aaactaaaat
    13261 agaatctcca aatgccatta ttaatagaaa gggaaaaaac tggtatataa gtgtatatga
    13321 ttgtattata acagtaaacg ctcacagtta tacaattatt acagctcata aagaaaagaa
    13381 atgaattgca aaatacaaag gtcaaaagca ttttccactg tttgtagtac ctatgaagaa
    13441 gtatataaaa taatattact tttgaataat aatttaaatt ttggaggttg aaaaaatgaa
    13501 tgatactgta aaagaaaaat tggcagagtc actaacttct gatactactg atattttacc
    13561 ttatttgcct tatttattac aagatttttg ggaattagga agttcaccag aacaaatgtt
    13621 acaattattt agaaagcata taccttttaa tgaagataca aaagttttgg atttagcttg
    13681 tggaaaaggt gctgtatctg tgaagcttgc aaaagagttg aatatacata tcaaaggtat
    13741 tgatttgata cctgaattta ttgattatgc gagaagaaag gctattgaat ataaggttga
    13801 tgctttgtgt gattttgaaa tgaatgatgc taatagttct gtaaaaattg agcacgatta
    13861 tgattgtgtg atttttggag ctgtagggga tattttaggt aattataaag aaactataag
    13921 taagctatta cctacaataa aaagtaaggg gttcatttta attgatgatg catatgtagt
    13981 agataaatct acaaatgaac agcttaattt tgagagggtg tatcccactt acaatgagtg
    14041 gtgtatattg ttcaaagaat taggtctgaa actaattgaa tgtataccat gtttagaatt
    14101 tgaaaatgat gttgatgagg aaatgaactg gattataact cgtgcaaatg agctaattaa
    14161 aaaatatcct tctcaaaaag agatgtttga acaatatgtt attagccaaa aaaatgaata
    14221 tactgattta cagcaggacc ttgttggagc aacttggatt ttacaaaaag aataagataa
    14281 atattagact agataatcaa tgttatagtg aattatacca tgtctagtga atataaataa
    14341 cataaaaaac cgtcaagatg aatagtttat tcgtgcttga cggtttttag tatttgttga
    14401 agtaaaattt tatttcttta gtatatcact tttagtcaat ccatttgatt tagcctgagt
    14461 attccattga tgattcccca taagtttata taggaatcta gggaagttag gtgaaataaa
    14521 aatagtactt ttgttggaat catctatatg tatattatca ctaagtatat tttttgaaag
    14581 ttcttttaat gcaattccta aatctttttt aggtccttta cccaaaggta cggattctag
    14641 tgaaccaaac atctccccag ctccaattcc aataccttga acccatgtaa agttgatttt
    14701 tttacaccaa tgctctatca tttgaattgc caaatgatta tgttcacctt cattgaatcc
    14761 acagttaact attgcataca ctggaatagg ttttttattt tgtgttttta aaaatttttc
    14821 acatttctct aaaaaatgaa gtaaatgaga tggaatacca tctacataaa gaggaaatgc
    14881 tattattaat ctatcacaat taagtatggc attaagagcg tcaggatact tagatgtgct
    14941 atctacttta aatgtaaaaa ctttattatc attttgaata agttgtttta attcagataa
    15001 aaaatactgt gaattatttt tatttacttt tggactacca tttatcatta ctatattcat
    15061 atggattcac ctcttatatc ttgtatattt tcatagaaac aaacagagtg ttcttttata
    15121 ttgaaattta tagcatttcc atttactaat ttcgtagcag tttctttttc agcttctgta
    15181 atacaatctc catagaatat agcttttaaa tttatctgtt tatcatatct acttttatga
    15241 cgcatcatat tatcttttat tacaaagtat ggaagaatat aattaatgct tttatcaagt
    15301 acgtttttga caaaagggct aaaccctccg taacagcatc tgcttattat aataattcca
    15361 tcagattcat ttataagttt acccatgttg ctgtaataat cttttacaac acattcagaa
    15421 ggagttttaa tccagcaacc aaaacaacca atacatttgt ggatttcttt tgtgtttgga
    15481 agtattttta tattgtcatc aagtttgggt aataactttt ctaattcttt gtcagttaaa
    15541 tcatgtatta aaagcctcat aaagtccatc tccttttatt atgtatgttt acttctaaaa
    15601 attatatctt atagattttt cagtaaatct ttacaccaat taataacaaa attataatag
    15661 tcaactccat aaatcattgt tgcatacata tatgtatatt ggtcaacaca atgattttct
    15721 tctgcatcat ttttttgttt acttaattta tttagaagta attctacttc agttattaac
    15781 atttttaagt ttgtttcaat tatttctttg ggtagatact tgtaaaagaa tatattaaca
    15841 aggtggtctt gctttgtttt agaaaaattt ataggtttct ttaaccattc aagaaatact
    15901 tctttaccag tattagttat ggaatatagt ttttttaatt tgttaccatc tataacttcg
    15961 tgatagtgta tatatccttt tgtttctaat cttttaagtg caggatagat acttccaaag
    16021 ctggcgtcaa aaaagtacga ggtactttct gacattattt gttttaagtc gtatccactt
    16081 aattcttttt ccattaaaaa tcctaaaata atgtattcta acatattacc tccttgtatc
    16141 aattagatat atatcgattc gatatattta ttattacaga taataaatct tacgtcaaga
    16201 tgctttttaa aataagctaa aaagaattag gagttattga taaatatgca aaaaaattaa
    16261 tataaaaggt aattaaatat caatgaagat ttaaaaatag ttaaatcaaa gtaagagtct
    16321 aatatataaa atttatagta actttatggt ttaaatataa acttgtgtgc tattgacaaa
    16381 gtataaaatt agcagtaaaa taattgcatg agcaaataga ttgttgcgaa cgcaaaaaaa
    16441 taaggacaat agatattaaa ggagagaata tgagagagct attaaaatgg gtttccatta
    16501 taaatagata caatacaaat tatattgaca ggaaattggc ggatattgga atcaataata
    16561 gccaatattt ttatgtatta cacatatgta ataatccagg tattacacag gacactatat
    16621 ttgagaaaat tcttgtgaat cctagtaata taacaagagc tttggcaaat ttagaaaaag
    16681 aaggctttat aacaagagaa ccaggtgtaa aggataaaag aacatggcat ttatatccta
    16741 cagaaaaatc tttaggatgt tatgatgaaa tactaaaaat aacaaatggg tatgttgagg
    16801 agttgttgag ttcatttgag aaagaagagc aagagttgtt tatgtcaatg ttaaagaaag
    16861 ttgcattttt ggctattgat atgaatgaag aagcaaaatt ggaggcgaag gaaaatggaa
    16921 gaaaaatata aggagccatt ggaatatgaa aaggtttcaa agttattaac taagtacgca
    16981 attcctagta ttatagcaat gttagtaagt gctttatata acattgttga ccaaatattt
    17041 attggtcaag gtgttggtgt attgggtaat gctgctacaa atgtcacttt tccactaaca
    17101 acaatatgta ctgcaattgc attattgtta ggaattggag gagcgtctaa gtgttcattg
    17161 gaattaggtg ctaaaaattc agaaaaagct acaaaagcag caggaaattc tatatgcttg
    17221 atggctgttt ttggaataag tttatttata gtagtatcaa tatttttaac accactatta
    17281 aaattctttg gctctacatc agaaatacta ccttatgcac acacatatac aaaaattaca
    17341 tcaattggat taccatttct tataatgtcc actggaatga gtaagttgat acttgcagat
    17401 gggagaccaa aagcttctat gttttgtatg ttaataggag cagttattaa tacaattttg
    17461 aatccattat tcatttttgt ttttaatatg ggaattgctg ggtctgcttt agcaactgtt
    17521 attgggcaac ttatatcttt tggaataagt gttcattata taaagaattt tcaacatatt
    17581 aaattacata agaaaagttt taaattagat agtataattt ataaacatat atttgcgcta
    17641 ggttcaagct catgttttaa tcaattagca atgactgtaa cacagatagt tatgaataat
    17701 acattggcat attatggggc taggtcagtt tatggaagtg agattccact tgcttgtgtc
    17761 ggtatcatta ttaaagtaaa tatgattttt atatctataa ttattggtat atcacaaggt
    17821 atgcaaccaa ttatagggta taattatggg gctgaaaagt ataatcgtgt aaaagaagca
    17881 tataagtttg cagtaggaag tgcaactatt atatctgtta ttgcattctt atgtttccaa
    17941 ttcttcccaa gacaaattac gtctatattt ggtaacggaa atgagactta ttttatattt
    18001 gcagaacatt attttagaac tttcttgttc ttaacattta taaatggtgt tcaaatagta
    18061 tcatcaactt tctttacatc aattgggaaa tcagtgttgg gactttttac ttctttgact
    18121 cgtcaaattt tatttttaat tccattgata gtaattttac caatgtttat gggaattgat
    18181 ggagtaatgt ttgcaggacc aatagcagat gcagcagcgg caatggtatg tatatacttt
    18241 gcagttcatg aaatgaaatc attgactata aagcaaaata atttgatgag taaccataat
    18301 aagattgcag aggtataaga caatatttaa gaagtataga aaattaattt aaagaagtat
    18361 aataaactaa tttaggaagt gtagaaaatt aatctaaaga agtataataa actaatttag
    18421 aaagtataga aaattaatct aaagaagtgt aataaactaa tttaggaagt atagaaaact
    18481 aatctaaaga agtgtaataa actaattt
//
LOCUS       DACXMZ010000062        18484 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.062, whole genome shotgun sequence.
ACCESSION   DACXMZ010000062 DACXMZ010000000
VERSION     DACXMZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.062"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     regulatory      39..141
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            235..1611
                     /locus_tag="JD016_08360"
     CDS             235..1611
                     /locus_tag="JD016_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAU4999651.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1725..2870)
                     /locus_tag="JD016_08365"
     CDS             complement(1725..2870)
                     /locus_tag="JD016_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAU4999652.1"
                     /translation="MNTFEQLKISSILIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            3123..3830
                     /locus_tag="JD016_08370"
     CDS             3123..3830
                     /locus_tag="JD016_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4999653.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3877..5472
                     /locus_tag="JD016_08375"
     CDS             3877..5472
                     /locus_tag="JD016_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4999654.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILGSIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            5575..6657
                     /locus_tag="JD016_08380"
     CDS             5575..6657
                     /locus_tag="JD016_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAU4999655.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            6806..6967
                     /locus_tag="JD016_08385"
     CDS             6806..6967
                     /locus_tag="JD016_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HAU4999656.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            7308..8576
                     /locus_tag="JD016_08390"
     CDS             7308..8576
                     /locus_tag="JD016_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAU4999657.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            8771..9559
                     /locus_tag="JD016_08395"
     CDS             8771..9559
                     /locus_tag="JD016_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAU4999658.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            9559..10008
                     /locus_tag="JD016_08400"
     CDS             9559..10008
                     /locus_tag="JD016_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HAU4999659.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            10012..12276
                     /locus_tag="JD016_08405"
     CDS             10012..12276
                     /locus_tag="JD016_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAU4999660.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            12416..12727
                     /locus_tag="JD016_08410"
     CDS             12416..12727
                     /locus_tag="JD016_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999661.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            13013..13513
                     /locus_tag="JD016_08415"
     CDS             13013..13513
                     /locus_tag="JD016_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAU4999662.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            13570..14556
                     /locus_tag="JD016_08420"
     CDS             13570..14556
                     /locus_tag="JD016_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HAU4999663.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            14606..15328
                     /locus_tag="JD016_08425"
     CDS             14606..15328
                     /locus_tag="JD016_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HAU4999664.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            15357..17291
                     /locus_tag="JD016_08430"
     CDS             15357..17291
                     /locus_tag="JD016_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAU4999665.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            17284..>18484
                     /locus_tag="JD016_08435"
     CDS             17284..>18484
                     /locus_tag="JD016_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999666.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKT"
BASE COUNT         7061 a         1829 c         3157 g         6437 t
ORIGIN      
        1 attaattata taaattaaaa taatattcgt aaagaaaaat tcatgttttg tactgtataa
       61 catataattt tgacaatatg ggtcataagt ttctaccgga ataccgtaaa tattctgact
      121 atgtatacta ttatttagtt aattaaatat ttaattaact gaattatgaa ggatacggct
      181 cagcttatca actactgtgt cgtttttttg taaaatttaa taggaggtgt tgttatgcaa
      241 acaacaaaca gtagtactat gcagaaactt tttccaattt tgactaataa aaatgtcgac
      301 atgaagaagg aaataattgc aggagtaact acttttttaa ctatggctta tataatagct
      361 gtaaatccaa atattttatc tgagacaggc atgcctgcag gtgcacttgt tacaggtact
      421 tgtttagcag cagcatttgg atgtatttta atgggtgtag ttgctaacct tccttttgct
      481 ttagcatcag gtatgggtct taatgcattt tttgcatata cagtagtttt acaaatgggt
      541 gttccttggg aagtagctct tacagcagta tttgtagaag gtattatttt tatagtatta
      601 tcagtaagtg gagttcgtga ggctgttgta aatgcgatac caaaaaatat gaaattagct
      661 gttacaggtg gtataggaat atttattgca cttataggtc tagttaacag tggaattgtt
      721 attggagacc aagccactct tatcaaaatg ggtagattta caccagcagt aataataact
      781 tgtgtaggat taataattat tgctgtatta gataaaaaaa gagtaaaagg ttctatactt
      841 tttggaatag tagtgagttc attacttgct tgggcatttg cttttataaa tccagagtat
      901 gcacaaaaat taggtatata tttacctggt ggattattta aattcgaaag tttagcacca
      961 atagcaggta aaatagattt aggatatgta ctacatccaa caaatatagg tggtttttta
     1021 gtagtagttt gtacattttt atttgttgat ttttttgata cagtaggtac attagttggt
     1081 gtttgttcta aagcaaacat gttagatgaa aaagggaatg ttcctaatgt aggtagagcc
     1141 ttacttacag atgcggttgc aactacagtt ggtgcaggac ttggagtatc tacagttaca
     1201 acttatgttg agagttcaac aggagttatc gcaggtggaa gaactggatg gacagcaata
     1261 acagttggaa ttttattctt agctgcaatg tttttctctc ctgtatttat agcaattcca
     1321 tcatgtgcta cagcaccagc tttaatatat gttggatatt taatgcttgg tacagttaag
     1381 gatatagagt ttgataatat aactgaaggt gttccagctt ttgttactat tgcttgtatg
     1441 gcattaacat atagtatagg tgatggttta actttaggta tattaactta tgttttcgta
     1501 aatatatttt ataatatatt tggagctaaa aaggcagaag acaaaaaaca tgtttctgta
     1561 gtaatgattg ttctggcaat attatttata ataaaacttt ggctactata ataggtatta
     1621 tttaaaattt taatttaata taaaataaag ggctgtaatt tattatcaat tgtgatgata
     1681 aactacagct cttttgtatt tgttgtattt ttgtatttat tgtattattt aataatgtct
     1741 attagttctc cattttgtaa agttttaggt gtaattacta tattgaactc tttttgcaaa
     1801 tcttttataa tatctaattc tttttttgtt attattgaga ttgtgttacc acttcgattt
     1861 cctctagcag ttcgaccaca tctatgcagg tattcatttt tactttttgg aaaatccaag
     1921 ttaaaaacat gacttacatc cacaatatct agtccacgag cagataaatc agttgtaatt
     1981 aaaatctttg ctttaccaag tttaaattta tttattgcat tctttctatc ttctttttcc
     2041 atatttccaa aaattcctat tgctttgtaa ttatgataat ttagttttga tactaggact
     2101 tctatatttt tttcattatt tacaaataca atagcttttt ttggatttgt agctgctaat
     2161 gcctttctaa gaaaagtaaa tttatccctt atctcaccta ataaatatga gtgattgatg
     2221 ttaggattta tttgtgattt ttcctttgtt tttattattt caaactcctt cataagttta
     2281 tcacaaattt ttatagtgct atcagtaaga cttgcagagc atcctataat ttgtctatct
     2341 cttaacgttg ttcttattat gtcttctata caagttatat tttttccatt caatagattg
     2401 tccacttcat ctaagacaat agtttttata ttatgtgatt ttagtttctt ttgttttatc
     2461 aagtctaaaa ctcttccaca agagccaatt actatgtgtg gttttactgc ttttatattt
     2521 tttatttgtt tttgtatatt gacctcccca attaaagcta aagaagtaac ggagagttct
     2581 gcatttttag ccaataattc aacctggttt gtaatctgca ttactaattc atgcgtaggt
     2641 gcaagtataa gagcttgtgt ttctcttttt gatgtatcaa ttttttcaaa tataggtaaa
     2701 aggtacgcta gtgtttttcc tgttccagtc tgtgaattaa tcaataaatc cttattttga
     2761 attatatttc ctataacaag tgactgaact tctgttggag aagttatgtc ttgttttttg
     2821 agtccatcta ttagaatgga acttattttt agttgttcaa atgtattcat attttgctcc
     2881 ttatatttta ataattatag tcaactattt tttattatac attgtttatt caaatcgaac
     2941 tatatattct tttataatta tgtgtaattt gaaagtaaat atcaattata tttatacctt
     3001 tatatttctg atacctttgc attttataga tttctaaaat aaacaaataa aagttgtaat
     3061 tgcttgatat ttgttatata ttatatataa caaatatcaa aactataata tggagggaaa
     3121 ttatgttaaa agtaatcaat gtatctaaat catataaaaa taaaaaagta gtaagtagtg
     3181 tctcatttga tgtaaatgat ggagagcttt ttgggtttat aggacataat ggagcaggta
     3241 aaactactac tattaaagca attgttggaa tacacgattt tgaagaggga gaagtactaa
     3301 taaattctaa atctataaaa aaacagcctg ttgaatgtaa aaaggaaatg gcatatatac
     3361 ctgataatcc agatttatac gagtcattaa ctggtataca ataccttaat tttatagctg
     3421 acatatttga agttgccaaa gataaaagag aagagttaat aaaatattat tcaaataagt
     3481 ttgaactcaa taaagcatta ggagatttaa tatcttcata ttcgcatggt atgaaacaaa
     3541 aattagctat aatttcggct ttgatacaca gcccaaaaat cttaattctt gatgaacctt
     3601 ttgtaggtct tgacccaaaa gcttccttta tattgaagga aattatgagg gagttttgtg
     3661 acaaaggtgg ttgtatattt ttctcaacac acgttttaga agttgctgaa aaaatttgtg
     3721 ataagattgc cataataaaa gatggaaata taattgctta tggaactaca gaagaagtta
     3781 agggaagtaa ttcacttgaa agtatcttta tggaattaat agagaaataa aatgtttttt
     3841 atacaatata gtgtctaaag tgaggaatga taaaatatga gcaatctaag tatattaata
     3901 aaaactaata taataaatga atttaaatta aatgcgttaa aaaaagctga tggtagagaa
     3961 aaacaaaaaa taatgggaat gatgctttca gttgttataa tagcgggtct attaattttt
     4021 tatgtaacga gcttaagtct ctctttagtt gatatcttaa agcaaataaa tcaactggaa
     4081 atgctactga tatttggttt tggattatca acattaacaa ctattgttac atcattatat
     4141 aaaacgtcat catatctatt tcaagcaaaa gatttagacc ttttgactag tttacctata
     4201 aaagaatcta ctgtactagc aagtaagata ttaatgttgg ttggagcaaa ctatttattt
     4261 tcttcaatat gtatgattat acctgcagtg gtttactttt taaattctga tgtaagtata
     4321 acttatttcc cttatttgat attgctattt ttagctttac cattattacc gatagttgct
     4381 tcatcaataa tttttctatt tatagggcag atatcatcaa gaataaaata caaaaattta
     4441 gtattaatca taggtagcat tatattgatg ttgctttata ccctacttat gtcaagactt
     4501 aattctatgt cagttgaaat gctaaagaat agtgtgtcta tttcagagac gattagaaaa
     4561 gtatatcctc ctatatatta ttttgttgat gctcttaaaa caggaagtat agtttcattt
     4621 ttagcattta tagccatctc aataatacca ttttttatgt ttgtaactct gtttgcaaaa
     4681 aaatttaaag atataaatgc taagatgagt gaaggatata agtcaaaaaa ttatgaattt
     4741 aagggtcaaa aaacttcaag accttcaaaa gctttattaa aaaaagaatt taagagatat
     4801 acttcttcgt ttatatactt tttaaattca tctgcaggtt tgttatttct aatagtaggt
     4861 acaattttaa tattattatt tggagcagat aaagtgggtg agttactgaa attgaatata
     4921 gattttaatc ttataaaaat acaattattg ggagtattct tctttataat agtaatgaat
     4981 tgtacaacat actgttcaat ttctcttgaa ggaaaaaact tatggatact gaaatcttct
     5041 ccaatagatg aaagagaaat attctggtca aaaatattga tgaatttttt attgaatgca
     5101 ccactatcta ttttatgtct aatattagta tctataagat tagactttga aattcagttt
     5161 attattgtat caatctgttt gattgtaagt ttttcaatgt ttgtagcatt gatgggatta
     5221 ttatcaaatt tattataccc aaaccttgat tggaaaaatg aggtggctgt agtaaaaaga
     5281 agtgcaagta tgatagttac aatgttagta tcattagctt atgtaattat acttggaggt
     5341 atatatgtat tacttggaat ttcgttttta aatatatata ttattttggg aagtatactt
     5401 actttgatat tagattttat gatatggaga attatttcta ctaaaggagt aagaatattt
     5461 aaaaatttat aggtgtattt atgtcaaata aagacttatc ttttttagtc ttgcaaataa
     5521 taggttaagt agtatactat gtatagtgat atgaaaaaat ttaggagaaa ataaatgaga
     5581 aaattatcca aaagagaaaa agattttatt caatatggat ttttattatt tttgatatgt
     5641 attactactt atttagtatt aactacttta gatataaaac taatacctaa aattataagg
     5701 ctagtaaata agacttatat atttcttggc atattgacaa ttatagtata tatgataatt
     5761 gaatctattg taactcattt gataataaat gcagttcaaa aaacaaaagt aaaatttatt
     5821 ggtattaaaa tggctacaat gggattttac tataacttag ttactccatt tgcatctggg
     5881 agtcaaccaa tgcaaatata tgcacttaca aaatatgatg taagccttag taaatcagtg
     5941 gctatagtta ctaataaaac tgtagtattt caaagtacag ttactgtatt ttgtggaatt
     6001 ttaattttgc taaataagtc attactgatg gagcaagtac attctgtgaa agtcttgata
     6061 acttttggaa tgattatgaa tgtaagtatg ttgttaggtg gactactaat agtatttagt
     6121 ccaaaacaag taaaaatgat agcagaagtt ttgattaatt ttttatctaa atttaaaaca
     6181 tttaaatttt tagattcaaa acgtgataga ataaatcatt atattgatga ttataattat
     6241 tctataaaaa tatttattaa aaatgtaaga atcttagttt taagtatatt acttactgtg
     6301 ttacaattaa tcgcatattt cagtatagtt tattgtgtgt ataaagcctc aaatttaaga
     6361 ggagtttcat atgtacatat aatgacatta caggtctttt tatatatggc tatatcacca
     6421 attccaacac caggaaatgt tggagctaat gaggttacat tttttactat gttctccaat
     6481 ataattccaa aagaattatt ggggtactcg gtactcttat atagtgggtt tgtatactat
     6541 tttatattaa tagtttcagg tttatttaca tttggaacgc attatacatt aaattcttgg
     6601 aaaaaggaaa ataaagtaaa ttctaattta gataaggtta ttgaaaaatt aaattaataa
     6661 aataattagg gaaattctat atataaatat ggtgttgaat aagctatatt atattgagat
     6721 atttcccttt tttgattaaa attatagaaa tttgaaataa taagtaataa acagaaaaag
     6781 tgatattgtt ttgaaaggag aaactatgaa acaagataaa caaaatgaaa aacaaagtga
     6841 gatttctaag ataaaacaaa gcaatgataa taaacttgaa atagctcaag aattacatgt
     6901 aagtaaaaat atagggaata tgcttggata tagaatagat aagaaaaaaa tgaatagacc
     6961 aagttaatga aaatgttgaa aatggtacat tttaaaaaat atatataatg aaatgtttta
     7021 ttgttaaatt taaacttatt ttaatggagg aaaaagtcga aaaatatcta aaaagatatg
     7081 gatataaaaa atattaatat atatatacta taaacttgac aaaatcaacc ttttaataac
     7141 tagcaaaaaa tagcaaaatt atataagcgt tatttaattg tcaaaaatga aagctaaatt
     7201 gcatgtccta aaaaaaatga ttgataacat ttatttgtgt gctataatta tatagtaaaa
     7261 caataagtat attgcataat gtatgcaaat cgggaggaga gacggacatg gaagcatcta
     7321 tacacacaat tgccagcaat aatttattga taatttttgc tattgtaagt ataactggaa
     7381 taatttgtag taagcttagt gaaataataa aggttccaga tgtagtgtta tatttacttg
     7441 taggtatttt aataggacca tcatttttaa aatttataga tattcgtgga tttcaaatag
     7501 aaaatcaact aatacttaca tttggttctg catttatttt atatttaggt ggaaaagaaa
     7561 taagtttaaa agtattgaga aatgtgaaga tatctgtatt cttattagct actttaggag
     7621 ttgtgatatc tgcttttata atgcaacaag taataggatt tacttttggg ataagtgcaa
     7681 tgactgcttt actagcagga actataatag catcaacaga cccagccact cttgtaccta
     7741 tatttaatca ggtaaagata aaggataggg taaaacaaac tgtaataagt gaatcagctt
     7801 ttaatgatgc aacaggagct atactaacat cagcagtgtt ggctgtaatt ttgtcaaata
     7861 aattttcatt aggtgaaaat atttatgaac tggggacaat ggttattgtc ggagttttag
     7921 ttggtttaat tacaggaata ctgcttttga aattagttaa tgataaacct tatggaatat
     7981 ttaaagattt tgcaccaata atatctgtta tttcagttgt agttgcatat gaattatcta
     8041 caaaattagg tggaagtgga tatatgtctt gctttattgt aggtataata actggtaaca
     8101 agaaaaactt taaaatttgg ttaagtcaaa aatcttatga tgcagatttt tatgttgctg
     8161 aaactttagg tactatttgt cgtatggcaa tatttataat attgggtagc caagttaatt
     8221 tggctcagtt aagtaaatat tttatgccat ctgtaattac agtattagta ttaatgttta
     8281 tagcaagacc actttgcgtt ttagtttgta cacttgtaga cagggaagca aaatggagta
     8341 aaagtgaaat attatttatg atgtgggtaa gggaaacagg cgtaatacca gcagcacttt
     8401 gtggtattat ttcagcaatg aaagttcctg gatacgaagt gatttcatct gtagtattca
     8461 tgactatact aattacatta gtaatacaag gaagtactac aaaattagta gctaaaaaac
     8521 ttggattatt agaagaagaa gtgataaata taagtgaaaa agtatcaact ttttaaagtt
     8581 ttgctttaaa atagtacctg ttaatggcta ttttaatatt gtctaatgat gattattgaa
     8641 atagtcattt ttttatgtat aagtaattta cagaatttac atattagaca tatttattat
     8701 caaaatttgt tgaagctatg atataattag ttgtatttaa aataagaata gcaaataagg
     8761 gaggaaattt atgtttacgg ttatggatat tgtacagcca gatacagtag aagaagcgta
     8821 ctcaatttta aataaaagaa aaactaacca agtaattggt ggaagtgctt ttttaagaat
     8881 ggggaaaaaa agaataggaa caggaataga attatctaaa ttaaatttag attatataaa
     8941 agagtatgaa gattacgttg agattggttc tatgactact tttagaacat tagaaacaag
     9001 ttccataata aaaaataatt ttggaagaat aatagaagat tcagttaaaa atataatagg
     9061 agttcagttt agaaatgtag taacagttgg tgcaacagta ttttctaaat atggtttttc
     9121 tgacttgata gtagcattac tttcattaga tacagaagta gaattatata atattgggag
     9181 aattagtctt gaagagtttc taaatagaga ttatgagaaa gatttactta ttaagatata
     9241 cataaagaag acaaataaga atgcttctta taaatcactt agaaatgcaa agagtgatta
     9301 tccaattcta aatgtatctg tatcaaaaca tatggaacaa tttaagttat gtgtaggagc
     9361 aagaccgcag aaagctacga ttgcaaaaca agctagtgaa tttctatcta acaatgaaat
     9421 caatgaaatt aatatagata aagcagtaga aatagcatca gaagaactaa cttttggttc
     9481 taacatgaga gcatctaggg agtatagaaa agctatgagt aaagtacttt taaaaagagc
     9541 tataatggag gttatttaat gttagtaaag attaatataa atgggaagaa aaaaagtgtt
     9601 gatatagaac cagaagaata tctggttgat actttaagaa aagttggtaa tttaagtgtg
     9661 aaaagaggtt gtgacacagg ctgttgtggt ttatgtacag ttttaattga caagaaacct
     9721 acattatcct gtgcaacact gacattgaga gctttagata aagaaataac tacaattgaa
     9781 ggtttagaaa atgaagttaa agaatttagt gaagttttgg taaaagaagg agctgagcaa
     9841 tgtggtttct gttctcctgg ctttatactt actgtacttg caatgaagga tgagcttgaa
     9901 aatcctacag atgatgaaat aaagcattat cttacaggaa atctatgtag atgtacaggg
     9961 tatatgggac agttaagagc tatcaaaact tatctggagg taaaatagaa catgaatata
    10021 gttggaaaag gaatcaagaa aatagatgga atgagtatca caacaggaaa gcctgtatat
    10081 acagatgatt tatcagcaaa agatgctttg gtggtaaaga tacttagaag tcctcacgct
    10141 tatgcaaaaa taaaagatat agatacatca aaggcagaaa tggttgatgg tgttgaatgt
    10201 gttttaacat ataaagatgt accaaaatct cgatttacac ttgctggtca aacatatcca
    10261 gagccatctc catatgatag attaattctt gaagatgttg taagatatgt aggtgacgaa
    10321 gtagctatag tggcagctat agatgaaaaa actgcaatta aagcaatgaa tcttatcaaa
    10381 gtaaagtatg aagtacttac acctgttttg gattttgaag aggcaatagg aaataaaact
    10441 atggtacatg atgaagatag acattgtaac tttgatatag gtatgcaaag agaaaaaaat
    10501 atagtatccc atcatgatta tacaaaaggt gatgtagaaa aagtattaag taattgtgat
    10561 gtagtgattg aagaaactta ttatactcaa gctcaagcac atgcaatgat ggagacgtat
    10621 agggcataca attatttaga ccatatggga agattggttg tagtaagttc aactcaaata
    10681 ccatttcatg taaaaagaca tttatctcgt gcactagaaa taccagctag caaaattagg
    10741 gttataaaac ctagaattgg tggcggattt ggtggtaaac aaactgcatg tgttgagatt
    10801 ttttctgcaa ttgttacttt aaaaacaggg aaaccagcta aaataattta tgatagaaaa
    10861 gaaactttta attgttcaac aagtagacat gcaatgagat taaaagttag actaggtgct
    10921 acaaatgatg gcataataaa ggcaatagat ataaaagcac tttctgatac aggagcatat
    10981 ggagagcatg cttctacaac tattggtttg gttggagaaa aaactttacc aatgtataat
    11041 aaagttgaag caacaagatt tattgcagat gtggtatata ctaataagat gccagcagga
    11101 gcacttagag ggtatggagc atcacaagga tgttttgctg ttgaatcaac tataaatatg
    11161 cttgctaata aattaaaaat ggacccaaca gatttaaggt taaaaaatgt ggtaacagaa
    11221 ggtgaaacta catttgcata tggtaaaact ttaaatagtg tcgatttaat aaaatgtata
    11281 aataaaggca aagaacttat tggatggaat gaaaaatatc cttgtaaaca tcttgataat
    11341 ggaaaaatta gaagtgtggg tatggcaatt actatgcaag gttctggtat acaaggtctt
    11401 gataagtctt cagctgaaat aaaattgaat gactgtggag attatactct tatgataggt
    11461 tctactgata tgggtacagg aagtgatacg attttagctc aaatggctgc tgaggttctt
    11521 gatacaagca ttgaaaatat aacagttata tcagctgata ctgacttagt tccatatgac
    11581 ccaggttcat atgcttcaag tactacttat gtaacaggta tggcagtagt taaggcagct
    11641 caagacttaa gagaaaaaat aatttctgct ggagcaacta aactaggcgt tgagaaggat
    11701 gaagtagaat ttgatggtat aaaattaact agtaaaaaag gtagcacttt attattcgat
    11761 atagcagttg attctacaat tggtccagat actaatcaat taataggtct tgcttctcat
    11821 ggtagcccag tatctcctcc accttttata gcaggttttg cagaaactga aattgataca
    11881 gaaactggaa aagttgattt agtaaattat gtagctgtag tagattgtgg aacagttata
    11941 aataaaaatc tagctaagat acaagttgaa ggtggaattg ttcaagggat tggaatggca
    12001 ttatttgaag aagttagata cacagaaaaa ggaagcatgg acacaaatac atttatgcaa
    12061 tacaagattc cagctagatg tgatattgga aatgttaaag tagattttgt tgaaacttat
    12121 gaaccaactg gaccatttgg tgctaaatct gttggagaag ttgttataaa tacaccttta
    12181 ccagcaatac aagaagctgt gtttaatgga attggtgtaa gaataaatac actccctatt
    12241 acacctgaaa aagtttttat ggaaatgaat aaataattat ttatgaaaat ttagactagt
    12301 tttacatgtt aaacaaccag ttatgtcata cctgttgaaa gatatttaat aagtagttat
    12361 tataatatta tgaattttac ttataaaata caaaattaaa taaggggatg atgttttggc
    12421 tggtaatcta aataatatga gagcagtaaa taattttaga ggagataaga acattttaga
    12481 atgtttagtc agctttgagg gtcgttcaat aagtcagaga aaagtaaggg tattttttaa
    12541 agaaaaacaa aatcaaatag aaattgattt tgcagaagag gaaatttcta aattggttga
    12601 aaatgttgtt ttaaatacat catatcaaga aatgttatat gatgaaatag agaaacaact
    12661 ggaaattgat tgtataggta cttggatgat attatctaaa ttaaaagatg gtagtagagt
    12721 tcactgattt taaaataaat tattagagcc taattttaag gctctttttt gctgttttta
    12781 attaacaata gaaaatttat ctttagtttg tatataattt taatatcata tattctatat
    12841 atataaaatt gaatttgtat gtattatgtt gtataatata aaatgaatta ggaaaatagt
    12901 tcgctaaaat aatttgtttt tataaaatta ctgtttaagt ttatagattt tgtataaaat
    12961 aaagttatgt tattaatttt tttagaaata aaacttttac taggagacag atatgagaaa
    13021 aaatagtatt aatatagcac taactgcagc aattacagca atctatgcag ttttaacatt
    13081 atcattggga tttataagtt atggaccaat acaatttagg gtagcagaga ttatgatgct
    13141 tttggcattt ttagataaag gatatatagt aggtcttaca ttaggttgtt ttttagcaaa
    13201 tgtaatagga ccatatggtg tacctgatat aatttttgga acattagcca catttataag
    13261 tgcaagtatg atttatatta ctagaaagtt aggtaaacag aataaaagta ctttagttat
    13321 agcttcttta tggccaactg ttataaattc gattgtggtt ggtctaatgt taaatgtatt
    13381 ctttgggctt ccattaattc tatctatgat acaggtaggt tttggagaat ttgtagtagt
    13441 aacaattgta ggagtaccat ttttctgctt tttaatgaaa aaatataaaa atgtcattaa
    13501 tattaaattt taaatagtgt atagtaattt aattaattga gagtattatt agtgaaaaga
    13561 ggtgtttatt tgtcgaaggt aaaaagattg actgatgttt ggatatatat taatgataag
    13621 caaaagttta caataaaaga gttgtcagag gaatttaatc tttcagctaa aacaatacaa
    13681 cgatatttaa aagaattaaa taaaatggga cttccaattc aagcagaaca aggtagaaat
    13741 ggaggttata gggtgctaaa taatagctat atacctcctg ttatatttac agaaaaagaa
    13801 gttatgatta tactatttgc acttaagtca cttcagattt ataattataa gttaatggaa
    13861 atagaaatta attctataat gagaaaagta agttatgaac gtaaaagcag tataaaagta
    13921 ggtatagaaa atattaaaag atacattggc tttgtagcta atgatactaa tgatggagat
    13981 tttaagagta gtaatgtaac tgagtttttt agagcttctt cagaatctct aattttaaat
    14041 attaagtata agtgtggtaa tcagattttg gaaaaaaacg tatgccctat aggtatatat
    14101 ttaaataagg gagcatggtt ttcaccagca tatgattgta aaagtgataa aataatatta
    14161 gttcatatag ataatataat tgatataaaa agaacaggag aaagtaaaaa aatacatata
    14221 aacttggaag aatggttaca tgaagtatat aacaatttat atggaaatag ttcaactact
    14281 aaaaaatttg gtgaaattat ttatctaaat gtattattaa ctaaagaagg tgtttctaaa
    14341 ataaaggaca gttcttggaa tttagatatg gagctaaatg aagatggtag tggaataatt
    14401 agtacattca taaaggttga agatatagat tgttatgctt cgattttatt ttttattgga
    14461 aataatgcta aggttataga gccaaaattt ttgaataagc tactttatga taaggccaat
    14521 gagttaagat atttttatga agaaaatatg gtataatatt tagaaatgtt gaaattaaat
    14581 aattattaag gagatatatg ttataatgga aaattttaat aagaatgcta aaataattgg
    14641 tattggagct gaaggtatta atataataaa tgaagtagaa gaaaaaataa aagcaaatat
    14701 ggatatagaa aaaataaaca taaatcaaga gattgaaaaa gaatatgtta gaagtctttt
    14761 agatggtgta gatatattat ttttgatata ttcatctgaa gataaacata ttagagatat
    14821 tataaaagct gtttcatata tgtcaaacga aagaagagtt ctatctatag gaatggattg
    14881 ttcagagaaa gaaaataaag aagatttaga gcttgggaga gagtttaaaa tcaataatga
    14941 tagtatattt aaatttgttg atttaatgaa tatcatggta gagtctattt ctgattcttg
    15001 catgataaat atagatatta ctgatttaaa agaagctatt gtaggagata aaggaattaa
    15061 gtattctttt gaagagtttg aagatacaaa gtcatatagc gagatagctg acattttatt
    15121 tgacaggatg gaatatattg gtgaggaatt tataagtaag aaaggaattg tatttgttga
    15181 aggaagtcca gaattttcta taatggaatt gaatgattta ataagtaata ttcagagcaa
    15241 ggtagaagaa agctatgaag taatattttc tttatatata aaggaaaatt taaatggaaa
    15301 tattagagta ggcttattgt ataattagta tattttagat attaaggaga gtgtatatgt
    15361 ctgaaaatat aagaggtaca gagtattggt taatggaaga atttttttat caggaaacag
    15421 acacaccaca gatttttaga gaagaaagca taattgcact tggatttgat ataaaaataa
    15481 atttaaaaaa agtattcagt atggggaaaa aatcagaaaa gtatattaat gagaattgta
    15541 atgataaaga ttttataaaa aagtttatgt cgattaaaaa aggagattat tttttatata
    15601 aagcatatca aaatatagaa gaaaaagtta aatgtaatgt gtctattggc atagttgaag
    15661 aagattttga aaatggatat gaattaagtc atattttagg gcatacacta ccagtaaaat
    15721 ggatgcataa ttttgataca gtttcaaatt gtaaagtata tacaagtgaa ttttataaat
    15781 taaataataa tgatattagc tcatatttag aaattattaa gtcacaagat aagaaattat
    15841 tgagaaaaag aatagaagaa agtgaaaata ataaatggta tttaaaaaac tattattata
    15901 aaaatgtaga taagtgtgta gatgatgaag aattatttat tcagcccatt aatctagaac
    15961 aattggaaag cctaaaaaat ataaagacag atgattatat tttattgtat aatagcgaga
    16021 aagtaatttg tgaaaatgac catacaatga agagtctatt tggcgtatgt caagttatgg
    16081 aagggttcga taaagagaaa gtaagtgctt tggaaggtgg atattttttt cctgttagat
    16141 ggatagagta tgaaaaggag aatatagacg gaaatgcatg ggttatattt gaaaataata
    16201 gtatagttga aaagtatgta aaaagattta ctaataaaat gccattaaat actatacttt
    16261 atggaccacc aggaactggt aaaacataca atacaaaaca gatggtttac aatctagtaa
    16321 gtgaaaccag tctaagaaac aaatactata caggttactt agattataaa aacaatgtag
    16381 aattttgtac atttcatcaa tcatatggat atgaggaatt tatagagggt cttaagtctg
    16441 atggtgaagg caattttaaa ccagaagatg ggatattaaa ggatatatct atagaagcat
    16501 gttataatgc attgaaattt gaaaaaaaga tgagctttga gttggaaaaa gatagcttga
    16561 ctttagaaac tataaaagac aggaaaaaga aattagtatt agaaaatata gaatatagtg
    16621 agagttttaa ttttgaagaa tcagaaaact atgttttaat aatagatgaa attaatagag
    16681 ggaatgtatc taaaatattt ggtgagctaa taacattatt ggaagatgat aaaagactta
    16741 ctaaatcaaa tcaaactatt gtcaaactac catattcaaa ggaaaaattt tctctacctc
    16801 caaatttgta tatagttgga actatgaaca catcagataa atcaatagca cttttagata
    16861 tagctttaag aagaaggttt aattttgaag aaattatgcc agattatgat ttattgtcta
    16921 ttgtagatgg tatagatatt agaaaaatgc tatatacaat caataaaagg atagagtttt
    16981 tatatgatag agatcatgtt attggacaag cttatctact tgctgtagaa aatattaatg
    17041 atatagtagt agtatttaga agtaaaataa taccacttct tcaagagtat ttttattatg
    17101 attcagaaaa agtaggtttg attttaggag gaattggaag aaatgaaaaa gataaataca
    17161 ttgtttataa agaaaaactt atagcttctg agttatttaa aaataataat gcctcaataa
    17221 tggagagtaa agttaactat tgtataaaac aaaacatatc tagtgaggag ctaaaaaata
    17281 tatatgagta aaaatatata tgttaaggaa acctatgaat ggataagggt aggtaatggt
    17341 gaaaatgagc ttacagaaat tgaatatgaa aaattgctta aatatctaga gaacaataat
    17401 gatgtattaa aaagtaatat aatagatatt aaatacaaaa aacttagatt tataaactat
    17461 gtaggcatca tatgttttga aaatgtaata cttgaaatac ttcctaaatt gtcattaagt
    17521 gataatcttg taaaagatag agagatacta ttacagatgc tttctatatg taataaaatt
    17581 ccaattacaa tgaatgaaaa aataagattg agtttgaaaa attataatct cttaaatttt
    17641 tttgttatgt atttcattga gagtatgcaa acacagatga aaaaaggtat ttattttgaa
    17701 tatataaata aaatagagaa cttaaatgtt atgaggggta aaatattatt aagtacatat
    17761 gcaaaagaaa aaggcatttc tccaatgaaa ataagatgtg aatatgatga gtatagtgaa
    17821 aataatttct tgaatcaagt tcttaaaaaa gcatgtatat ctattttatg tagaataaat
    17881 gacaattcaa ttcaaggcaa gattaaaaaa attttaagct attttcaaaa tgtagattta
    17941 atttatatag atagaaaaaa actattggat tataaatttt ataaaaacaa tgatagattt
    18001 aaagattgtt atttactagc tagacttcta cttttaaatt tgtctatgga taatagtcaa
    18061 aataatcaag aggcattttc tatattattt gaaataaata ctttgtatga agaatatata
    18121 ggaattttaa tcaaaagtat atgggacaat tcatttagag aaacatatat acaagataag
    18181 agtaaatttc ttttgaaaaa tgaacaaaca ggtaagaaaa actttaattt aagaccggat
    18241 atagttttat atgacttaaa aaatgaatat gaaattataa ttgacactaa atggaaagct
    18301 attgaagtag attcaaatgt tttttatagg tcaagtgata tataccagat gtatgcatat
    18361 ataacagcat atgaaaatgc aaaaagatgt atattattat atccttgtat tcaaaaagat
    18421 aagaattata gttcctggaa attatcagaa tcatttaagg gtaagtttat agaagcaaaa
    18481 actg
//
LOCUS       DACXMZ010000063        18477 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.063, whole genome shotgun sequence.
ACCESSION   DACXMZ010000063 DACXMZ010000000
VERSION     DACXMZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.063"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            110..1270
                     /locus_tag="JD016_08440"
     CDS             110..1270
                     /locus_tag="JD016_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HAU4999667.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1612..1773
                     /locus_tag="JD016_08445"
     CDS             1612..1773
                     /locus_tag="JD016_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999668.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            1966..2595
                     /locus_tag="JD016_08450"
     CDS             1966..2595
                     /locus_tag="JD016_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAU4999669.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2834..4093
                     /locus_tag="JD016_08455"
     CDS             2834..4093
                     /locus_tag="JD016_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance protein"
                     /protein_id="HAU4999670.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4372..5346
                     /locus_tag="JD016_08460"
     CDS             4372..5346
                     /locus_tag="JD016_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HAU4999671.1"
                     /translation="MNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIKVIKHNNY
                     ICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGIGLDELIL
                     GNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDFGITKANN
                     GVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNSEEKMLQS
                     IDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKAYIEVEDE
                     KEEEQKQDKVIQVKKEYKSKMRKKIHKKLVVAGLAIVMISMIIISSII"
     gene            5592..6659
                     /locus_tag="JD016_08465"
     CDS             5592..6659
                     /locus_tag="JD016_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAU4999672.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7193..9103
                     /locus_tag="JD016_08470"
     CDS             7193..9103
                     /locus_tag="JD016_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAU4999673.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9166..9789)
                     /gene="ytaF"
                     /locus_tag="JD016_08475"
     CDS             complement(9166..9789)
                     /gene="ytaF"
                     /locus_tag="JD016_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HAU4999674.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            9977..10621
                     /locus_tag="JD016_08480"
     CDS             9977..10621
                     /locus_tag="JD016_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU4999675.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLIQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            10835..11893
                     /locus_tag="JD016_08485"
     CDS             10835..11893
                     /locus_tag="JD016_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAU4999676.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            11988..13181
                     /locus_tag="JD016_08490"
     CDS             11988..13181
                     /locus_tag="JD016_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAU4999677.1"
                     /translation="MKNLKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILID
                     QETGRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSS
                     YKLKDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTH
                     FLNPNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDR
                     SFEKSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVINKNDKNEKKRRVIGVVLGTN
                     HKNKRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILK
                     DDESIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLK
                     MKFAN"
     gene            13388..14101
                     /gene="rpsB"
                     /locus_tag="JD016_08495"
     CDS             13388..14101
                     /gene="rpsB"
                     /locus_tag="JD016_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAU4999678.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14180..15091
                     /locus_tag="JD016_08500"
     CDS             14180..15091
                     /locus_tag="JD016_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAU4999679.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15176..15880
                     /locus_tag="JD016_08505"
     CDS             15176..15880
                     /locus_tag="JD016_08505"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAU4999680.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            15906..16463
                     /locus_tag="JD016_08510"
     CDS             15906..16463
                     /locus_tag="JD016_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAU4999681.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            16466..16669
                     /locus_tag="JD016_08515"
     CDS             16466..16669
                     /locus_tag="JD016_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999682.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            16698..17432
                     /locus_tag="JD016_08520"
     CDS             16698..17432
                     /locus_tag="JD016_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAU4999683.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            17445..18224
                     /locus_tag="JD016_08525"
     CDS             17445..18224
                     /locus_tag="JD016_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAU4999684.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
     gene            18199..18462
                     /locus_tag="JD016_08530"
     CDS             18199..18462
                     /locus_tag="JD016_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999685.1"
                     /translation="MRLNFLLDKDWLDLKEQSIIASNENSYVTFKCRDILQLLVTWLF
                     SLFKNLEDLGELYKNRYYFIIVYYICSEYIFYTITIIKKYFFV"
BASE COUNT         7594 a         1846 c         3216 g         5821 t
ORIGIN      
        1 agtatgatat agataaaagt atttatttta attgtctatt atctgaaaaa gtctttactt
       61 agaaatcata aaaatcaaat taattaggaa aagaaagggt gtgtataatg tgattgagac
      121 tggatttgta tattgtagtt tcttggttgt attcgtaggt gtaatttttt ttatggttga
      181 taaattgaaa tggaaggtat ttgatgttat taatccaatg cttttaatat acttaggttc
      241 agctatactt ggatcatttg gtttatttgc acagaatgaa gatgttggaa cgtatcaaaa
      301 cataataaca gtgaacttcc ttccattaat gttagtgttt ttgttaattc aatgtgattt
      361 aagaaaaata ttaaagatgg ggcctaaaat gttattatca tttttctgtg ctacatttac
      421 atttatgctt ggatttgtta tagcattcat aatttttaaa aattcaatgg gtgcagattc
      481 atggaaaatt cttggggcag catctggttc atggataggt ggctcatcaa atatgattgc
      541 agcagctaat gctctaggag ttgcagaaga aggacttagt tatgcaatac ttatgggttc
      601 tataggatat acagtatgga tgtctgtttg tctaatgtct gtgaaatttg gacctaaatt
      661 taacaaatgg acaaagtgcg atacaagttt tatagatgag gttacatcaa aacttgaaaa
      721 agaaaataaa gataatacgg cacctacgtt tgtagaactt atgattttac tttctgttgg
      781 gtttggagtt gcgtcttttg cacaaattgt atccaagata cttccaactt caggtatact
      841 taatgaaact atgtgggtag tacttgttgc atctacagta tcaatattat tgggtatgac
      901 aaaaatatca aagcttaaag ggagtacact ggtaggaaat gtgtttatgt atttactcct
      961 tgcagctaca ggtagtaaag caaatttctt tggattgtca gaagcacctg tttacataat
     1021 gtttggattg gtagtagtcc ttgtacatgc tatttcattt atagggcttg ctaaactatt
     1081 taaacttgat ttatttactt gtgaagtagg aagtatagct aatataggag gagtaactac
     1141 agctccaatg attgctgggg ttcacaatcc tgtattaata ccagtaggtg tacttatggg
     1201 gcttctagga aatattatag ggacttattg tgctcttatg gtaactcaaa tattacatat
     1261 gttagcttaa taaaaatatg gtataaaatt aataatttaa ataagaaaag tcatttcaat
     1321 ttaagcagaa gcttttactg aaatggcttt tttgtttgac aagataatct atttcaagaa
     1381 gatgttttga catatttagt gtataatatt gtaaaattta ctaaaaacga gatatattta
     1441 atttttatat tgaatcgaac gtctgtatga tgtataataa atatacagaa cgaatgtttg
     1501 cagaacgggt gttttttgaa tttattttaa taatttatga atatatatca gaatgcataa
     1561 atatatttaa atataaagat taaaaagaat ttaaaggagt gttgataact tatgataatt
     1621 ccatcaagaa gaatacaaga tataacatta acaaatttaa aaaatggtga agtaacttta
     1681 attgaattgg atgaaatata caagaagatg ggatttttat ttgttgttag tgaaggcaaa
     1741 ttgaaaaaga taaaaaagga gaatcatcat tagaaatgaa tgaaataata aatattataa
     1801 tagtttataa aacctaactt atgtagaata taaatactta agttaggttt ttttgtgata
     1861 aagtttataa gtactcaatt ttataagata aataatggtc ttaagtgaaa agtaaataag
     1921 tttaaatttt agtaagtatt atattgcata agaggtgata ttattatgaa aatagtaacc
     1981 tacaatattc ataaaggtat ggattcaaat aatagattaa ctctaactaa aatggggtta
     2041 tatttaaaac aattggattg tgatgtaatt tgtttgcaag aagtattata tcctcaattt
     2101 ttagctttaa aagctgtttt aaatatggat ggtgtctttg cgacaaatgt aaaaaaagta
     2161 aatatgatat atggtatttg tacatttaca acatctaaaa tgttaaacag taatcatttt
     2221 ttcttaacta gcaaaaaaga acaacgggga gctctttgta taactataga tgcatatgga
     2281 agaattataa atgtaataaa tactcacttg ggacttgata ggcaggagag agcaaaacaa
     2341 ttagacgaaa taatagacta taggaacaga ttagtgggga tagttatttt atgtggtgat
     2401 tttaatgaga aaaatgtctt tttaagtatg tttaatgata tggctatttc actaaacaag
     2461 tcttacttac caacatttga aaaatcaaac tctagaatag attacatttt tgttaataaa
     2521 aatactgaac taaaaggata cacagtagaa aaaatttacc tgtcagacca ttatcctgta
     2581 ataggttaca tttaaatttc aatataaatg aaattagttg ttataaaata aacaattttt
     2641 tgttaagaaa tagtaaaaga aatataataa aaaatatcaa aaataaaact aattataaga
     2701 cataaattta caataaagtt acatttgatt aaattccttg tttaacaaaa gctattcaaa
     2761 tataatctaa ggtagttttg aaatgggaac tctattatgc aaatttaagc tagtgttaaa
     2821 atgaggtgaa gttatgggca gaagaacaac aagagtttca agattaggta aagaaaaaaa
     2881 acgaagtgtt ttaattgtat tggcagcttt agttctaata atgggtggat ttacttattt
     2941 ttttaacagt aaatttctgt acaatgggaa aatcgctaaa aatgtatata tagaaggagt
     3001 aaatgtatct gatatgacta aagcagaggc attaaaagct ataacagata agtatactcc
     3061 agaagattta aatcttgctt atgatgggaa aaaatataca atatcaccaa aagatattga
     3121 tttaaaatat gatacagaag atgttgtaaa agatgcttat gaaagtacaa aaaaagggtc
     3181 ctattttcaa aacttaaaaa aatatattga tataagagtg aataaggcaa atatgaagat
     3241 aaaagctgaa tatgatgagg caaaacttag ttccaaagtg tcttctatag cagatagtat
     3301 aaatgttaag atgaaaaatg cttctattag tgttggaagt ggtggactaa gttatacaga
     3361 ctctgttgta ggtagagaat ttgatttggc agcaaataaa gagtctatat ataatatgat
     3421 aaaaaataaa gagcataaaa ctttagaact taaagtaaat cttcaaaaac cagatataac
     3481 aactgaacaa gtaaaaactg taaattctgt tataggtcaa tattcaacta cttattcaca
     3541 agctgtagag ggtaggtcat ataatgtagg attatcagca agaaaaacta gtgatgtatt
     3601 attaatgcca ggtgaagaat tttcatacaa taaacttaca ggacctagta ataaagcaaa
     3661 tggttacaaa gatgcacctg ttatagttta tggtaagcta gaacaatcag caggaggagg
     3721 tgtttgtcaa acatcttcaa cagtttataa tgcggctctt ctttcgggta tggagataac
     3781 tcaagttaca aatcattcat ctgcatctac ttatgtgcct aaagggagag atgctactgt
     3841 gagcgatgga ggattaaact taaagtttaa aaatccatat aagcatcctg tatatattaa
     3901 aaattatgca ggtggaggaa gtgtatcatc agtaatctat ggtaattctg gtgacaaacc
     3961 aaatatatct atagaggtaa aacaaactgg tcagaataaa tattctacat atcgaatatt
     4021 taaagattca agtggaaagg tcataaaaaa agaacatata tcaaatagtt cttataaaga
     4081 attaaaaaaa tagataattt tttagagaaa tatttgtaat ataaaaggga gtgtctcaaa
     4141 atgaactttt tagtttagag acactctttt tctgtgaaat caaatatatt ttcatcattt
     4201 caacaataca aaagaagcgt atctctattt tgagacagcc ttttttgtaa aaattaacac
     4261 tattagttct tgttttaata ttacaatcat taatatatag aaaatattcc tatatattgg
     4321 tataattgta aatagattta gttatataaa atgtatgagg aggtgacatg gttgaatttt
     4381 ataggcaata gatatgaggt agtaaattct gatgacgttt tagaaattaa caaaatctat
     4441 aaagctagag atgtttttta caggaaaaat gtgttgataa aagttataaa gcacaataat
     4501 tatatctgtg aagattttgt ttcaaattta attgatgaaa gtactgcttt agatgagata
     4561 aactctcctt atatactaaa aataatagat gttgggattc actgtacaga agaaacaaca
     4621 ttatattatg ttgtaagtga agactttaat ggtatcggac tagatgaatt aattctaggt
     4681 aattatcttc atttagaagc aattgtaaac ataatgatac aagttttaaa agctttagaa
     4741 atgatacata ggaatcattc atatcatggt agtttaaaat cgagtagtat aatagtagat
     4801 acagaataca atataaaaat ttgtgatttt ggaattacaa aagcaaataa tggggttaat
     4861 acaagaagct atggaaacag gagatattta tgcccacatc aactatgtat aaattatact
     4921 gataaagaaa gtgatttttt tgcaacaggt cttattctat ttgaatctat atttaaaaaa
     4981 ttgccttttg gagaatcaaa ttcagaagaa aaaatgctac aatctattga taagggatta
     5041 gattggaata gtataaaagc aattaatgga aatactgaac ttataaatgt tataaaaagg
     5101 cttcttggaa gaaataataa gtataccaga gccaatgata ttattattga tttaagtaaa
     5161 gtaatgtatg aaaaagcata tatagaagtt gaagatgaga aagaagaaga gcagaaacaa
     5221 gacaaagtta tacaagttaa aaaagaatat aaaagtaaaa tgagaaaaaa aatacataaa
     5281 aaacttgttg ttgccggtct agctatagtc atgatttcaa tgattattat tagttccata
     5341 atataaaaat tgtatattaa gtattttaaa atcaatgtct ataaaggata tacatagaaa
     5401 ttttagtgta gtcttaaatt atgaaaggaa gtggttatta tgttaacaaa aaataatgag
     5461 gtattaaacg tctaggagta ttttaacaaa tagccacctg cttttacagt ttagaataaa
     5521 aaagctgtgg ctaaaggtca cagctttttg ctacttttta aaggcaggag aatattaaaa
     5581 aggagcaaat tatgagcaat ataaaaataa tagcaagtaa taaatgttgg attgaagata
     5641 tagcaaggaa acaacttgaa gatatttcaa agttaaatgg aattttaaga gtagtaggat
     5701 tacctgactt acatgcaggt aaaatacctg taggtttagc agttgagact aaaaatatta
     5761 tttatccaca tataattgga aatgatatag gatgcggaat gactttattt aaaactgggg
     5821 ttctaaaaaa gaaatttaaa aaagacaaat ggattaagtc acttagtaag attaaagatt
     5881 tgtccgatat agaaataaaa aacacatata aagaagaatg tccaatttta aatttaggaa
     5941 ctatagggag tggaaatcat tttgtagaaa ttcaatgtat aagtgaaatt tataataaag
     6001 aacaatttga acaattaaaa ttttcatctg atgatattat gatgttagtt cactgtgggt
     6061 caagaaatta tggagaagat attttaaaaa aattttatga taaagatgga cttgaagttg
     6121 aaagtgagaa agctatagat tatattaaaa aacatgataa tgctttaatg tgggcagagc
     6181 gtaatcgtga agctatcagt aaaaaaatta tgcagtcaat aggtacatct ggagatgttg
     6241 aaacaatatt ctctataaat cataatttta tagagaaaag ggaagataaa tttatacata
     6301 gaaaaggtgc tgtttcaagt gaaagaggag ctgtgattat accaggttca agaggcagtc
     6361 tatcatatat agttatgcct actgaaaata cagagatatc tttatattct ctatcacatg
     6421 gagcaggtag aaaatggtct cgttcagttt gtaaatctcg ccttaaaagt aagtatagta
     6481 aagatactat aaaacaaact aaatttaaaa gtcagattat atgtaatgat gtaaatttat
     6541 tatttcaaga agctccagag gcatataaga atatagagca gattattgaa agtttgatag
     6601 aatatgaact tattcaagta gtggcaacta tgaagccatt gattacttat aaaggataat
     6661 ttttaaagtt aaaaaagatt aaaattatat tataaatagc taattttatg gaacatttaa
     6721 aagtgtttta ttattagcta tttttttatt ttgtagagac gataatttat agagctaaat
     6781 tctttgatat ataatgatta caaagttaga ctgtattgga aactgcacat acatgaattg
     6841 atgacaatat aagtgattcc aaaatataaa tgaaagtata agcttatttt cactataaaa
     6901 attttcactg tacaaaaaca ttaatgacat tatttttatt gacataaata tataatttat
     6961 ggaataatat tagaagcact gatataagtg tttaaataag aatagaaaag gagaagtgtt
     7021 ttatgaaaaa aaatactaac attaatgagt ccggagttaa acttcttaat aaataaggag
     7081 tttgatatat aataactcct tatttattaa gttgttcgca aaataactat tatcaaagta
     7141 aatgataggg tgatttaaaa gacgatgact tataaataaa ggagaaaaaa taatgaatag
     7201 taatacattt gaaagattac aactaaatga agttaaagac ttaataaaaa tacattgtgt
     7261 aagttcgcta ggaaaaaatt taatagataa attaactcct agtgggaata ttggagtagt
     7321 aagaaggaaa ttagcagaaa ataaagaagc tagaaagata attgaaaata gtaatcacat
     7381 accacttgaa ggattgttta atgcaggttc tacaatagat aaaattgaaa aaggaatgat
     7441 aatagaacct gttgaacttg taaatattga agatttttta agaggttgta ggaagatgaa
     7501 agcttttatg ttagaaaaag aattttattc acctacatta agttcttatg ccttaaatat
     7561 aacagaatgt aaaagtatag aagatgagat aaattactgt ataaaatcta ataaagttga
     7621 ttctaatgct agtaaagaat taaaaaagat aagacgaaat atagaaatta cagaaggaaa
     7681 gataaaagat agattaaata aatttataac atcaactgta aataaaaagt atatacaaga
     7741 gtttataata agtaaaagaa atgatagata tgtaatacca attaaatctt cttataaaaa
     7801 tgaagttaat ggtactatat tagatacatc atcaaaagga aatactgtat ttattgaacc
     7861 tattagtgtt tcaaatttaa gtacagagtt aactatgtta aaagctgatg aaactattga
     7921 agaatacaaa atattatctt atttaacaga acttatattt gagaagataa gtcagataaa
     7981 attaaatata gagatacttt cagaatacga tatggtattt gcaaaagcta agtatagtca
     8041 aaaaataaaa ggaattactc caaaaataaa taataatggc tatataaaaa taattaaagg
     8101 taaacatcca cttctaacag gagatgcagt acctttagat tttgaaatag gcaaaaatta
     8161 tagaagtctt ataataacag gtcctaatgc aggaggaaaa actgtaaccc ttaagactgt
     8221 tggtttacta actttaatgg ttcaatgtgg atttgatatt tctgcaaaag aaggtagtga
     8281 atttagtgta tttgaaaaag tttttgtaga tataggggat aatcaaagta ttgaaaatgc
     8341 cttaagtact ttttcatcac atataaaaaa tattgcagag attatgagtt tatcaaataa
     8401 ttctactctt gttctgtttg atgaaatagg aagtggaact gaacctaatg aaggagctgg
     8461 tttagcaata tcattattag aagagtttta caaaatggga tgtataacta tagcatcaac
     8521 tcattatgga gagataaaaa agtttgctac attgcatcca gaatttgaaa atgctggtat
     8581 gatgtttgat aaagaaacgt tagaaccatt gtataagtta actattggaa aatctgaaga
     8641 tagtaatgct ctttttattt ctaaaaaaat gggaataaaa aatagagttt tagatagagc
     8701 taaagaatat gtaattgata gaaactacaa tttagattta gttaaaagaa gtaagctaca
     8761 atctaatatt gaagataaaa atagttctga agtcatttta aactatacag attataatgt
     8821 aggggataag gtaaaattac tagatgaaga tgattttgga attgtatata agcctatgga
     8881 caagtttaat aatgtagaag tattgtttaa tgataatttt atttcagtaa atataaaaag
     8941 attacaactt agcataaaag catcagaatt gtatccagaa gggtatgatt tagatgtatt
     9001 atttactagt tttaaagaaa gaaaattaga aaaagatatt caaagaggtt caaaaaaagc
     9061 tcttaaaaag attcagaaag aaattagaga gaatagaaaa taaatataaa aagcacaaaa
     9121 gaaagcaatc taaagagaaa agtttatctt tagattgctt tcttattaac atatcttcat
     9181 aaatccaatg actaacaaga ttaaaccact taaccaagat aagtttaaat ttacgttttt
     9241 aacaaacata tttcctagta aacaaccaag taatactgca attatatttg atattaaaga
     9301 aaatagaata acttgtatat aattcacacc tacaaggcca ctaccaaaac cgatagctaa
     9361 tccatctaaa cttaaagcaa gtgcaagtgt aaatgcttca gcagatttaa gcactttaga
     9421 tttacttgta tctaagacat caggttcgat acttacatta aaatttaatt taaggtctaa
     9481 catattaaaa ttataattct ttgagtgtat agattttttc ttaaaataag atttaaaaaa
     9541 actttcaatt agtctagcac ttcctaataa aaataaaata aagaaacata ttaacattgt
     9601 tatattttga ggtaaaaaat ctctaactat tgtacctgca aaaagcgata ttgctaaaat
     9661 agctgaacaa actacgctga taattatatt tgaatataag ggaactttaa ctttatttac
     9721 gccatatcca aagcttgtca caaaggcatc tatacaaaga gaagtaacta ataaaattga
     9781 ttcaatcaca gtagattaac atcctttctc ttaaattttg tacttatatt agttatagta
     9841 ttgtaaaaat ggataaattg ttacagataa tccaataaat tgttgataaa atttcaacat
     9901 tatacaacac ctaaataaca aaagaatcat atcatataat aatataaaac caatcataat
     9961 aaagcaggtg aaaataatgg aacaacatag taaggttggg aatttcggtg atttgttgaa
    10021 agttttatta gatgaaaaag aattatcaat gggagaacta agtaaaaaaa gtggaattga
    10081 taaatctact atatctcgta tagctaataa taaacaaaag ccaaatataa atcatttaga
    10141 aaagatggca atacatttaa atattaacct agaagaactg ttgaaagctt caggatatga
    10201 atttagaaac agcaataatc aaatatttaa tgcagatagt gattttagta actttgatga
    10261 tattcttgga tttgcaaatt taatcaatga taagaacttt aatggaaata ttgaaaaaga
    10321 gttaagtaag tgtaagttat atgtccaaac agatgaaggt aaaaaattac tttttgataa
    10381 ttttaataaa aaaatagatt ctattgaaaa acaaggtcaa tttacagata gattaataca
    10441 aatgtatgct gatttttgta cagatggact tacaataaag aaatatttat tgattggaag
    10501 tatgctactt tactttgtta tatcaacaga tgtgattcca gacttcgtgt tcccaatagg
    10561 atttatggat gatttagtag cattaaatat agttacaaaa ttacttaaaa atgataagta
    10621 agttttagaa tcatatttaa tatataaata aatcaaagta tataaaattt attatttttt
    10681 atatgaaaca aattaaaagc acaatgaagt agattaaaaa tttaaaacta agaatctaat
    10741 atatattttg taactattta agtatataaa ataaaataat atccaaacta atacaaaata
    10801 gtaataattg ttttgtactg gaggatatat atttatgaat tgtaaatgga tatctaaaat
    10861 agatgagaga aaaaaatgtc atagagaagc agattcttct ggatattgta tctttcataa
    10921 agaaaataag tctgatgaag agatacagtt gatgatggat actcttcata aagaagagat
    10981 aagtgaattt aatggatttg tttttgaaaa tgaatttaat gcggaagaaa tattaactta
    11041 caattataaa atactggatt tttctgaatc catatttaaa caaaaagcaa attttaaaaa
    11101 atatattttt aaaaagaata ttatttttaa ctatacagaa tttagagata aagttttatt
    11161 taatggttgt gtattcttag aaaactgtga ttttaataga accatattta gtaaacacta
    11221 tataaatgac cgtatatttg aaaaagtgaa atttaaagga cctgatttgg tagttaataa
    11281 agtagaaaat tttccacgaa tggatggaat aatttttagc atgtgtacaa aatttgtgtt
    11341 aaagaatgtt gaatatggaa aaagtgagta tgagcatggc aagataaatt atagaatagc
    11401 tagaaatcaa gcaacaaaaa ttggagaata tgagatgata ggattttact attataagga
    11461 aagaatttat agtagtaaaa taatgaaacg ttctaattat cctactttca gtgattattt
    11521 agttgaaaaa ttttttgacc aaatagcacg ttacacaaca ggttatggag aaaagccatg
    11581 gaatatatta ttagttatta tagcaataat aagtgttttt gctttattat acttatttgt
    11641 aggcatagaa agttcaaata gcacacttgt agctttagat ataaataata taggtgatta
    11701 ctcactaagt gagattttca aaatgtatat ggatttatgg tattttagca tggctacatt
    11761 tagtacagta ggatatggag atatggttgc tacaagtttg attgggaaag cattggcagg
    11821 tatagaagta ttttttggag ttacaatagg agcaatttgg gcatctgtga ttattaaaag
    11881 aatgataagg taatatttgt gtaaatatgg ataggtatta attactagat ataaattgga
    11941 ctaaataggg aaaatttaaa tataaatata cttaataatc caagaaggtg aaaaatttga
    12001 aaaatgcact aaaaaaaatt atatcattag ccatgattct agtttttata ataccaaatt
    12061 attcatatgc agatgataaa tctgtagaca agtattcaaa aacatctata cttatagacc
    12121 aagaaacagg tagagtttta tatagtaaag aacctgatat gaaggtacca ttagctagtt
    12181 taagtaagat gatgactttt ttacttgcaa tagaagctat tgaaaataaa gaagtaaaag
    12241 aaactgataa agtaaaaata gacaaatcta ttgcatcagt aaaagggtca agttataaac
    12301 ttaaagatgg tgaagaagtg ccacttatag agcttatgag aggtcttatg attgtatcag
    12361 gaaatgatgc ggcaattgct atagcaaaac atatttgtaa aacgaaagaa gcatttgtag
    12421 atagaatgaa taaaaaagca aaagaaatag gaatgagtaa tacacatttt ctaaatccaa
    12481 atggtcttcc aatatacgat ttaagcaacc ccaaaaaacc agcaaaagaa aatatctcta
    12541 ctgcaaaaga tatagctatt cttggaaaat acatgtttga ccattatgaa aaacaagtaa
    12601 cagctataac tgatatggag acatatacaa attcagatag aagttttgaa aaaagcaata
    12661 caaatgcatt attaagaatt ataccagaag tagatggtat aaaaactgga tatactggca
    12721 atgctggata ttgcttatca ttttctatgg ttataaataa aaatgataaa aatgagaaaa
    12781 aacgtagagt aataggggtt gtattaggta ctaaccataa aaataaaaga acctctgctt
    12841 cattagctct actgaaatat ggaaaagaaa atttcaatat gaaaaaagta attgataaag
    12901 atagctttat tggtaagaaa tatataaaag gaatgaaaga gttagaggtt acattaaaag
    12961 caaaagatga actttataca atattaaaag atgatgaaag tataaagtca gaagttaaga
    13021 ttaaaaatat agagtaccct gttaaaaaag gtgatactat gggtattata aaatattata
    13081 ctaattcagg tgacttactt ggaagtgtag atatagtaag taataatagt atagataacg
    13141 tatcattaaa aacaaaatta aaaatgaaat ttgcaaatta aattcaaaaa atggtataaa
    13201 atacacaaaa aaatataaaa atagaaaata tggaaaaaat attgttattt cttcacctct
    13261 atgatatact actagaggtg aaaaaataaa ataaatcaca cacagcattg ggatccttaa
    13321 aaggtgccta ttacatggtt ttttagggag aagatcaagc tggaggtata aaaccaggag
    13381 gtaagatatg tcagtaatat caatgaaaca attattagaa gcaggtgttc actttggtca
    13441 ccaaacaaga agatggaacc ctaaaatggc taagtatata ttcacagaga gaaatggaat
    13501 atatataatc gacttacaaa aaacagttaa aaaagttgaa gaagcttaca agttcactaa
    13561 agaagtagct gaaacaggaa agccaatctt attcgtagga actaaaaaac aagctcaaga
    13621 tgcgataaaa gatgaagctg aaagatgtgg aatgtacttt gttaatgaaa gatggttagg
    13681 tggaatgtta acaaaccaca aaactataaa aacaagaata aataaattaa gagaattaga
    13741 aaaaatggaa gaagaaggcg tatttaatgt tcttcctaaa aaagaagtta taaaattaag
    13801 agctgaaaaa gaaaaactag aaaaatactt aggtggaata aaagacatgc ctgaattacc
    13861 aggtgcaatg ttcgtagttg acccaagaaa agaaaacata gctattcaag aagctcatag
    13921 attaggtata ccagtagttg gtatagttga tactaactgt gacccagaac aattagactt
    13981 tgctatacca ggaaatgatg atgctataag agccgtaaaa ttaataactg gtgctatggc
    14041 aacagctgta attgaaggta gacaaggtgc tgaggaagaa gtagctgaag accaagaata
    14101 ataatttgac aattaaatgg taagggtcgc ccttaccatt tataatatgg aatagaataa
    14161 gtaggaggaa acttaaaaaa tggctaatat aactgctcaa atggtaaaag agttaagaga
    14221 aagtacaggc gcaggaatga tggactgcaa gaaagcttta caagaagctg aaggaaacat
    14281 ggaaaaagca gtagatttat taagagaaaa aggattatca aaagctgcta aaaaagctgg
    14341 tagagttgct gctgaaggtc tagttgcaat agaaatgaat gatgataata cagttgcttc
    14401 tatggtagaa gttaactcag agacagattt cgttgctaaa aacgaagact tcaaagtgtt
    14461 cgttaaagat gctgcttgta tggcattagc tactgataaa gaagatatag cttctttatt
    14521 aggtgaaact cataaagagg gaataacttt acaagaagtt ttaaataata gagttgcaaa
    14581 aataggtgag aaattagact ttagaagatt tgctaaagtt gtaactaatg gacaagttgc
    14641 tggatacatt cacggtggtg gaaaaatagg tgttcttgtt gaaatggaaa cagaagctag
    14701 agatgctaaa gttttagaat taggtaaaga tgtagctatg caagttgctg ctatgaatcc
    14761 taagtatgtt tcaagagatg aagtagatgc tgaatacata gcacatgaaa cagaagtatt
    14821 aactcaacaa gctttaaatg aaggaaaacc agctaatata gttgaaaaaa tggttaaagg
    14881 aagattagaa aaagaattaa aagaggtttg tttattagag caaactttcg ttaaaaatcc
    14941 agatataact gttaaacaat tagttgctga tgttgctaag gcagtaggtt ctgatataaa
    15001 agttgttaaa gttgtgagat tcgaagttgg tgaaggtata caaaagagag aagaaaactt
    15061 tgcagaagaa gttgctaagc aacttaagta ataaccaaag agagaacacg caggtgttct
    15121 cttttttaaa aaatagaaaa aattggatat gaaggattga aataggaggt ttctgatgga
    15181 taaaccaatg tacaaaaggg tattgttaaa attaagtgga gaagcattag caggagaaag
    15241 aggttttggt ataaataatg atgtagttaa tgatattgct attgctataa aaaaaataca
    15301 agaaataggt gttgaagtag ctgtagtagt aggtggagga aacttctgga gaggcagaac
    15361 cagtgaagga atggacagaa ctactgctga ctatatagga atgttagcta cagttatgaa
    15421 tgcaatggcg ctacaggatg cactagaaaa tatagatgtt gctacaagag ttcaaactgc
    15481 tatagacatg agacaaattg cagaaccata tataagaaga agagcagtta gacacttaga
    15541 aaaggaaaga gttgttatat ttggtgcagg aacaggaaac ccttacttta caacagatac
    15601 aactgctgct ttacgtgctg ctgagatgga agcagaagtt atattgttag ctaaaaatgt
    15661 tgatgcagta tatgacaagg atccaaaagt acatgcagat gctaagaaat ttacagaatt
    15721 aagttacatg gaagttatac agaaagaatt aaaagtaatg gattctacag ctacatcttt
    15781 atgtatggat aataaaattc caataaaggt atttgagctt acaacagaga atataataag
    15841 agcagtaaaa ggcgaaaata taggaactac tgtaaaataa tgaagaatga aggaggagta
    15901 aaattatgaa actggaaata cataagcaat tagaagagaa aatgaatggg acaattgatg
    15961 ctttaaagtt tgaatttgga actataagag caggaagagc taatgcacaa atgctagaca
    16021 aaataagagt tgattattat ggaactccaa ctccaataaa tcaaataggt gctatatctg
    16081 taccagaacc aagaatatta atgatatctc cttgggataa atctgctatg catgaaatag
    16141 aaaaagcaat agctaactca gacttaggat taaatccatc aaatgatggt gaggttataa
    16201 gactttctgt acctgcttta acagaagaaa gaagaaaaga attagctaaa aaagctagta
    16261 aagctgcaga agaatttaaa gttagaataa gaaatgaaag aagagacgcc aatgagaaga
    16321 ttaaaaagat ggaaaaaggc ggagaattaa ctgaagatga attaaaaaaa gcacaagatg
    16381 aagttcaaaa aatgactgat aaatttataa aagaaataga tacattatta tctaaaaaag
    16441 aaaaggatat aatggaggta taatattgaa aaaatattat aaccttttag gtcttcatgt
    16501 agatgaggta aaacaatatt ttgatgagca aaatataaat tataccgtaa aatctattga
    16561 aggtaaaaag gataaagaaa aacttgtagt tcctagagtt ataaaaatat ctgagatagg
    16621 tgatagtgta gaactgataa tcacatactt ttctgactcc ttagtttaag gagtcagaac
    16681 aatattggag gcaaaatatg aataacaaca ttatatatga tatagactta aataacatac
    16741 ctactcatat tgctattata atggatggaa atggaagatg ggcaaaagct agatttcttc
    16801 caagaactgc aggacataag gctggagtag aaactataag agatatagta aaagaatgct
    16861 caaaattagg tgttaagcac ttaacattat atgcattttc aacagaaaac tggaaaagac
    16921 cgaaacttga agttgacaca cttatgaact tattatctac atacttaaga aatgaaatag
    16981 ctgaacttca ccaaaacaat gtaaaagtaa ctgctatagg agatataagt gctttaccaa
    17041 aaacttgtat aagagaatta aactcagcta aggaactgac aaaaaacaat acaggagtta
    17101 acttaaattt agcattaaac tacggaagta gagcagatat aaaaaatgct cttatagata
    17161 tagtaaaaaa ttgtgaaagt ggtaaaatag atataaacaa tattgatgaa gatattataa
    17221 aaaactattt aagcacaaag tctataccag atccagactt ggtaataaga acaagtggtg
    17281 aacaaagatt gagtaacttt ttactttggg aagttgctta ttctgagttt tactttactg
    17341 atatacattg gcctgatttt aaaaaagaag aactacagaa ggctatatac gtatatcaaa
    17401 gaagggatag aagatttgga ggacttaagt aaggagatta ttttatgctt acaagaatta
    17461 ttgcttcact agctctggtt ccattatttc tatttgttgt atatggaggt attcctctat
    17521 atatagctga gacagcaata gtttatattg cattgcatga gttttataag gcatttaaaa
    17581 taaaagatgt acatccaata ttcattatag gatatttatt ttctatttat ttagctgtaa
    17641 aaaatatttt taatttacca ttagaatata cttatgcagt aatttttatt ttatttttag
    17701 caagtataat ttatatgttg atgggcaaaa acaatgttat agatgtttcg attacgtttt
    17761 taggagtttt ttatatagga gtatttttag attttataat tataactata aatggatttg
    17821 aaaaaggtag tatatatgta tggcttatat ttgtgatatc ttttatgaca gatatatttg
    17881 catattttag tggttattta cttggaaaac ataagctaat tcctaaagtt agtcctaaaa
    17941 agactataga aggagctatt ggaggaataa taggaagtac actttgttgt attttatttg
    18001 gatatttatt tggcattgat ttgcttcaat tagcgataat aggaagtata ggaagtgtaa
    18061 tagctcaatt aggagattta tttgcatctt ctataaagag atatgtaggg ataaaggatt
    18121 atggaaagat aatacctggt catgggggta tattagatag atttgatagt gttatattag
    18181 ttgcaccttt tgtatatagt gcgattaaat tttttattag ataaagattg gttggattta
    18241 aaagagcaat ctataattgc tagtaatgaa aatagctatg taacttttaa atgcagagat
    18301 attctgcagt tattagttac atggctattt tcattgttca aaaatttaga agaccttggg
    18361 gaattgtata agaataggta ttactttata atagtatact atatttgtag tgagtatatt
    18421 ttttatacta taactatcat taaaaaatat ttttttgttt aataatttcg atatttt
//
LOCUS       DACXMZ010000064        18185 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.064, whole genome shotgun sequence.
ACCESSION   DACXMZ010000064 DACXMZ010000000
VERSION     DACXMZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18185
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.064"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..528
                     /locus_tag="JD016_08535"
     CDS             <1..528
                     /locus_tag="JD016_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAU4999686.1"
                     /translation="NKPQFISNLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVARLQ
                     EHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRSISDVSWSEFIRQLE
                     YKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWIYPSCNETHDRDINASI
                     NILKEGLRLITIQNK"
     gene            652..819
                     /locus_tag="JD016_08540"
                     /pseudo
     CDS             652..819
                     /locus_tag="JD016_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            960..1214
                     /locus_tag="JD016_08545"
     CDS             960..1214
                     /locus_tag="JD016_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999687.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1241..1573
                     /locus_tag="JD016_08550"
     CDS             1241..1573
                     /locus_tag="JD016_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999688.1"
                     /translation="MNDMARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSI
                     IVTIFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVK
                     KILIDYII"
     gene            1847..1999
                     /locus_tag="JD016_08555"
     CDS             1847..1999
                     /locus_tag="JD016_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999689.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2193..3115
                     /locus_tag="JD016_08560"
                     /pseudo
     CDS             2193..3115
                     /locus_tag="JD016_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589412.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
     repeat_region   3382..4399
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3382..3410
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4554..4742
                     /locus_tag="JD016_08565"
     CDS             4554..4742
                     /locus_tag="JD016_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999690.1"
                     /translation="MNKIATKSRAEYMKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNL
                     TKKEWLEEKIDEELEQKE"
     gene            4863..5228
                     /locus_tag="JD016_08570"
     CDS             4863..5228
                     /locus_tag="JD016_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HAU4999691.1"
                     /translation="MKNELMMFEGKEIEVFEFEGRILFNSKDVANCLDIKNVNENIRL
                     MNEKQVVKLRNSDISNTDIRKLNNAGENFLTESGVYKLIFKSRKEEAERFQGWISDEV
                     LPSIRQTGAYINNNAIPKS"
     gene            5282..5677
                     /locus_tag="JD016_08575"
     CDS             5282..5677
                     /locus_tag="JD016_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999692.1"
                     /translation="MLKELLDDAGFDNKSKLLTAKTLYKKAGIDLPIEINEEEHYFDT
                     KQIASKLKIYSKSNKPAQMAVCEIIKKIDLEDGEVKGVWETNGSWTGTVNKYTKSVID
                     KVRTWIEENNRPTKIADEKKNYHVFYKIE"
     gene            5729..5890
                     /locus_tag="JD016_08580"
     CDS             5729..5890
                     /locus_tag="JD016_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999693.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            5966..6190
                     /locus_tag="JD016_08585"
                     /pseudo
     CDS             5966..6190
                     /locus_tag="JD016_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905847.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
     gene            complement(6219..6683)
                     /locus_tag="JD016_08590"
     CDS             complement(6219..6683)
                     /locus_tag="JD016_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999694.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYKNNIQFYTTVLDVLEDMKKKILRERKYKKRR"
     gene            complement(6819..7136)
                     /locus_tag="JD016_08595"
     CDS             complement(6819..7136)
                     /locus_tag="JD016_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999695.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITILAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(7419..7715)
                     /locus_tag="JD016_08600"
     CDS             complement(7419..7715)
                     /locus_tag="JD016_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999696.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKSYLNRF"
     gene            complement(7734..8147)
                     /locus_tag="JD016_08605"
     CDS             complement(7734..8147)
                     /locus_tag="JD016_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999697.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(8131..8364)
                     /locus_tag="JD016_08610"
     CDS             complement(8131..8364)
                     /locus_tag="JD016_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999698.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            8583..8957
                     /locus_tag="JD016_08615"
     CDS             8583..8957
                     /locus_tag="JD016_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAU4999699.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRALVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            9569..10261
                     /locus_tag="JD016_08620"
     CDS             9569..10261
                     /locus_tag="JD016_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4999700.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            10236..10442
                     /locus_tag="JD016_08625"
     CDS             10236..10442
                     /locus_tag="JD016_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999701.1"
                     /translation="MVIRLKNFSGKIIKEKSLIFNCILILVIFTFTQMLGILICFNFF
                     SESLIYSMGIMLFLNFILYKILTM"
     gene            10448..12517
                     /locus_tag="JD016_08630"
     CDS             10448..12517
                     /locus_tag="JD016_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAU4999702.1"
                     /translation="MGNLEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTS
                     TKSGIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSS
                     DSEYDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNV
                     EIINKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQ
                     IFKQATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVI
                     LVSISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSI
                     IVRVILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIF
                     LGFTIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRA
                     IEEQVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKS
                     KRLKLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPN
                     DKVYIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNF
                     DPSEIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYE
                     EEITNME"
     gene            complement(12723..13046)
                     /locus_tag="JD016_08635"
     CDS             complement(12723..13046)
                     /locus_tag="JD016_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAU4999703.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(13039..13740)
                     /locus_tag="JD016_08640"
     CDS             complement(13039..13740)
                     /locus_tag="JD016_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAU4999704.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(14018..15403)
                     /locus_tag="JD016_08645"
     CDS             complement(14018..15403)
                     /locus_tag="JD016_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAU4999705.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIGFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(15511..16749)
                     /locus_tag="JD016_08650"
     CDS             complement(15511..16749)
                     /locus_tag="JD016_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HAU4999706.1"
                     /translation="MELFIEVIKFNIYISPLILFFILFTKKISKYTFKFNYFICLLIV
                     VRMMFISKINLNILNINNTTINIYKHNIDNYIPYDQGITLDIFMYIWILGIIAISFYY
                     LRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTR
                     CKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIYWFNPILYLLKKYFH
                     ELCELSCDEIVIDKCTTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKKNIILKKRL
                     NSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGT
                     HIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFK
                     KTIPLYDKEGIKIIGRFEIN"
     gene            complement(16762..17130)
                     /locus_tag="JD016_08655"
     CDS             complement(16762..17130)
                     /locus_tag="JD016_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAU4999707.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
     gene            complement(17658..18080)
                     /locus_tag="JD016_08660"
     CDS             complement(17658..18080)
                     /locus_tag="JD016_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAU4999708.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
BASE COUNT         7076 a         2023 c         2652 g         6434 t
ORIGIN      
        1 aataaacctc aatttatctc aaatcttaag aagtcattaa acaaacttgc taaattacaa
       61 agagaattat caagaaaaac aatcggtagt ttaaatagaa ataaagcaag attaaaagtt
      121 gcaagacttc aagaacatat agctaatcaa agaaatgatt ttctacaaaa actatctact
      181 aaattaataa aagagaacga tattatttgc atagaagatt tacaagtaaa gaatatgatt
      241 aggaatcgta aactatctcg ctcaatttct gatgtgtcat ggtcagagtt tattcgtcaa
      301 ttagaataca aggctaattg gcatggtaga caaattgtaa aggtgggtaa attctttgca
      361 agttcacaaa tatgcaataa atgtgggtat aaaaacgagg aagtgaagga cttaaatata
      421 agagaatgga tttatcctag ttgtaatgaa actcacgata gagatataaa tgcaagtata
      481 aatatattaa aagaaggatt aagactaata acaattcaaa ataaataacg tatgtaagaa
      541 ccgtaggaac tacggggata gcctgtggag aattagtaag acatatgtta gtatgcaaaa
      601 ttctgtgaag caggaaacct gtgactttag tcatgggagg ttcaggctag tattactcat
      661 tattgtattc aaaagctaaa gtggaaacct agtgaatata tgaatttaga agttaattag
      721 agagcattag cagccgactc aatacttatt aaaattgaag atgaagaaga aacaatgaaa
      781 gaagctgaaa gagagaaaaa gagaggtaga agaaggtaga atttaaagtt aaaatagtta
      841 cattgctaat atatgaaaat gttacaaatt tggaaataga tggatatata tgttgtatag
      901 tataatgtag ataatatatc accaagtaaa aaacaacaaa taaatctaaa ttatgagggg
      961 tggaaaataa agatattaaa cttacaatat gtatatttgc ttttgtaatt ggaataatag
     1021 tgttattttg ctgtataggt ctaggtcaat ttaatatgga ttgtattata aaacaaaatg
     1081 gtgggtcaat agctaccgaa caatacaaca tatatttaga acaatctatt tcacaatata
     1141 gaaattttgg ttctatactg gttttactag gaggaatagg aatactgttt gataaaagtg
     1201 tcagaaaata ctaaaaaagt atatttttat ttaggatgtg atgaatgata tggcaagaga
     1261 tgtatttagt gtaaagatga caccactaga agcacagaaa atggtagaaa aaaatttgga
     1321 agcagattta gtttctgttg atatttataa cctagaaaat aataagtcta taatagttac
     1381 aatctttgaa aaatattatg caagaagcaa tagtgatgca ggtttggtag tgatttgtga
     1441 aaatactaca ggaaaaacac ttgttaaaat tacctcaact ggtagtgcaa ttggactatt
     1501 tcagattgat tggggaacag gtaataattt gattaaaaga gtaaaaaaaa tacttattga
     1561 ttatattatt taattatttt atgtagatat tcttaggttg gtatggaatt tgtactaacc
     1621 tattaagtaa tcaataaaac taaataaaca tagaaagcac ttacaaatat gtaggtgctt
     1681 tttattgaaa aaatagaaaa taaaataaaa agtaagatat aggaaaaata tgtaagaatt
     1741 atatgttata ataattgtag caagaagatg taatctacaa tttatagagt ggagttcatg
     1801 tattaaaaaa ttatcctccc aacgataaga agggaggtga atatacatgg ataatttttt
     1861 atttaatgtg ttagctagtg taacagctag ttacatagtt tatttgatta gcaagttaat
     1921 caaaaaagta aaaagccact ctaccgcaaa gagtgactta aagattgata taaattttaa
     1981 atatcatcgt aaaaaataac tttcagttat agaacttcac tctaccgcaa atagattgta
     2041 gttcttcttg tttttattat accacaaatt agaaaaaata ttgcatataa tattttttat
     2101 agtcaataaa aatatgaaat tttttataca caatgatgat gaatctaact agatgaataa
     2161 atttaattgt aaataagaaa gtacttactt aaatggtagg tgcttttgtt ttgccaaatt
     2221 atgctataat attgagtgag atgagtaatt tgtgggggaa ttttataatg tggatattag
     2281 taccaataat aacaatagta ttacttataa tagctgtttc ttctatgcaa tatattttag
     2341 ttatgatagc ttttttgttg attatatatt cttttataga aaagaaaata gttatgggcc
     2401 ttgtgtcagt tctttttttc acttattcaa tttatctttg tgcaacttgg gaagataaat
     2461 ccttgatagc agataataaa gttgagactg ttaaggcaca aagagaaact gtagaaagag
     2521 aaaaagaaat ggaaagaaga agaatacaag aagaagtaga caaagaaaga tatattgaaa
     2581 aacatggaat ggaaatatca gaaaatgatt taaaagttaa gctagaggtt ttagtacctc
     2641 aagaatataa ggggaagaaa tatgaattaa aagtaggaaa atttaaaaga tatagtatgt
     2701 attttgattt aactgtacaa aatgaaaaat ttagtaattc tgaataatgt aaaaaatttg
     2761 taaaagaaat tgctaatgat cttaaaaaaa ttaaaataag caaggcttat tttaattttc
     2821 attcaaaaga tgacggtggt atatataatt ctgtttatat tgattatttt agaaacatac
     2881 aaaataatgt agataatgtt gaaaatttag aattcaatga gtttgaactt aaaaccgaag
     2941 aagaagagaa gcgagaacaa gagaaaatag aacaagaaaa aacagttata ataactacat
     3001 tcaaaacaga gtggttgacc cactagatag aataaagaaa ctaaaagagt tgctagattc
     3061 aggagcaatt acacaggaag aatataataa aaagaaaaaa gaattattag aatagataat
     3121 atagtaagca cttacaagta tgtaggtgct tttaaattta caaattatta cattttaatt
     3181 ttatagttta gattttatga tataataaaa atatagaagt tttgcagtgt gcgatatttg
     3241 ttacaaagta gggcttaata cttgaaatct aagatgttga gggtgcgtga taagtgttat
     3301 caattgcact attgcccgct cactgcaatt ttaagagtat tgtatatatg taggtattgg
     3361 aaatgctaag tttattttgg ggttttagat taactatatg gaatgtaaat tttgtacttc
     3421 cgctttcgct gttgaccctg ctccttgttt tagattaact atatggaatg taaatcgctt
     3481 tcagtagctg tcgtttcgtc tggaatatta aagttttaga ttaactatat ggaatgtaaa
     3541 ttagcttgat aataaccacc ctccatagga ttatcagatg gttttagatt aactatatgg
     3601 aatgtaaatg atgacatgat agtattagct aatacagaag taaaacgttt tagattaact
     3661 atatggaatg taaatttttt ttcatcattt aaaacttcaa ctatcttata atgttttaga
     3721 ttaactatat ggaatgtaaa taatattatt gcttttatat tttttaaatt tatacttgtt
     3781 ttagattaac tatatggaat gtaaatttgc attctcccaa ccattctaga tattctccat
     3841 agcgttttag attaactata tggaatgtaa atgcgcaaac attaccactt ccaccttttc
     3901 caaataaacc gttttagatt aactatatgg aatgtaaata gccttgtctc aatatgaaag
     3961 agatttaaga atgccaggtt ttagattaac tatatggaat gtaaattgag ttaatagaaa
     4021 atttaaatac taatgaaaaa gtgttttaga ttaactatat ggaatgtaaa taatttttcg
     4081 agagtctcaa tttacagaag aaattggagt tttagattaa ctatatggaa tgtaaatttt
     4141 taagcgtgtt gcaatatgat tgccttatgt ttggttttag attaactata tggaatgtaa
     4201 attcggttta ttcgtattat atttctataa tcttatttca gttttagatt aactatatgg
     4261 aatgtaaatc ttgatgattg gcgagaatac tatatttgtt ggtcagtttt agattaacta
     4321 tatggaatgt aaattgctgt gatttcacta ataaaacaat taataattgt gttttagatt
     4381 aactatgtgg attcaaaatt aaataaacaa agaaagcact tacaaatatg taggtgcttt
     4441 tattctgctc aaaattggtt ggttgggtaa aataattaga aaaagttagt aaaaacctat
     4501 tgactgtaac tcgttacaat attgttatta atgtaacgag ttacagaaaa gaggtgaata
     4561 aaatagcaac taaaagtagg gcagagtata tgaaaaatcg tcgaaaagat aaaagaggtt
     4621 ttagtgtact tttagacaaa gaaaagttag ataaatttga tgaagtatta gaggaaaaga
     4681 atctaaccaa gaaagaatgg ctagaagaaa aaatcgacga ggaactggaa caaaaggaat
     4741 aaaaaataag ggtcactccc accgaccaaa gtttgagtaa cccctatgac gtatactatc
     4801 gtatatcaat tataatatat gtcattcctt aaaaaaaatc aattattaag gagtgtaata
     4861 ttatgaaaaa tgaattaatg atgtttgaag gaaaagagat tgaagtattt gaatttgaag
     4921 gaagaatttt attcaactca aaagatgtag caaactgttt agacataaaa aatgttaatg
     4981 agaatataag acttatgaat gaaaagcaag ttgtaaagtt aagaaattcg gatatcagta
     5041 atactgacat ccgaaaactt aataatgctg gagaaaattt tcttactgaa agtggagtgt
     5101 ataaattaat ctttaaatca agaaaagaag aagctgagag attccaaggc tggataagtg
     5161 atgaagtttt accaagcata agacaaactg gtgcatacat aaataataat gcaataccga
     5221 aaagttgaga gaaaaagcaa gtgagattga aaaattacag ttagtttata acagtacatc
     5281 tatgttaaaa gaattgctag atgatgcagg ctttgacaat aaatcaaaac tattaacagc
     5341 taaaacatta tataagaaag caggaattga tttaccaata gagataaacg aagaagaaca
     5401 ttattttgat acaaaacaaa tagcatctaa actgaaaata tattctaaga gtaataaacc
     5461 agctcagatg gctgtttgtg agattattaa aaagattgat ttagaagatg gtgaagttaa
     5521 aggtgtttgg gaaactaatg gttcttggac tggtactgta aataaatata caaagagtgt
     5581 aatagataag gtgagaactt ggatagagga aaataataga cctaccaaga ttgcagatga
     5641 gaagaagaat tatcatgtat tttacaaaat tgagtaaatt tatcagttgt attaaataat
     5701 ttagtttagt tttgtgggga ttaatacaat gtgtgagaat ttacttgata atatgtatat
     5761 tgaaaagaga aaagaagaat atagaattaa acttttgaaa ataagagaaa cagatataga
     5821 tatatataat aagttagaaa gtatagtata taaactttct gagaaaaaat tagagaaaaa
     5881 taataaatag acatataaag cacttggata ttctactgtt tcaagtgctt tgtttggtat
     5941 aaaatggtat aatggagata gagaattata ttaacccaaa aatggagaaa atagagaaga
     6001 tgatttagtt gacgctttat cagtaggagt acttgcaggt aagacagaat ctctagtacg
     6061 attagttgga gatggaataa gtgatactca aaaaagttta ataaataaaa gagaatttag
     6121 aaatataact caaattggcg gaaatggaaa tgagaaagca tttagtgaag tagaaaactt
     6181 agttaaatag attagaaggt agcaactaga gttagttgtt accttctttt tttatacttt
     6241 ctctctctaa gtattttctt tttcatatct tctaatacat ctaatacagt agtataaaat
     6301 tgtatattat ttttataagg tatagctatt ggaagaatgc ccttgttaac taaaaaaata
     6361 agaagtacaa taaataaaag tgtcataatc attctaataa aataattagt gatatctgat
     6421 aaatcaaatg ttaagttaat tgttgtagaa aatacaacag ccataataga tatatacaaa
     6481 gcaaaaatgg ggttactatt tttttgtaac tgtcctttaa gcctagattg ctctatttct
     6541 atatcttgaa tactttttat ggaatccatt tcactataat acttataaac ttttaaataa
     6601 tattttttat acttgtttaa agcttcttca ttatctttaa ggtctttgtc acttaactct
     6661 gatttaactt ccccaaaatc tataaaatcc ctcctacaat gttatataat atactttaat
     6721 ttatagcata tttaagaact tatatcagca atttgtcgaa caattttagc taaaaataga
     6781 gtataatgat aggcttatta tttataagat attatttttt atatagttat atatttcttt
     6841 ctaattagat aatataatgc tgttgttata atggctacta atattattgc taatattgta
     6901 atatctaatc ttcttgtaaa taagcctatt gctatatact caagacctgt tacatagcga
     6961 taaagtactt ctagtttaac agtttttttc ttatctatat ttccatatat tgattctaat
     7021 ttattaaaat attttttact acaaaaagca gttttaggtt taattaaata tattattgta
     7081 gttcctatta taactattat tccaataagt ataagattga tatttccaaa atccatagca
     7141 gatacctcct attaaatttg cttttcttaa tcaaagtata tcagtaacac aagtacaaag
     7201 agtatctaaa atattacaaa tattaacagg atagataaaa ctatagtaaa aattgtaata
     7261 ataaagcgaa cgaaaattat catactaaaa cataagacat gttataattg tattagatag
     7321 aagattaaca tacaccaaag cactccttat aataaagagt gcttattttt ttgaaattca
     7381 tcaatatata aactatcaag aatcttattc aacacacctt aaaatcgatt taaatagctc
     7441 tttttgataa cataatttat atgatataat aaaaaaatag aagtgtacca ccaatacact
     7501 tctatagcta taactaagca ttctcttgct gggggagtgc ttttttcatt tctccaaaca
     7561 acattattta cttcatcagt aattatcaaa tcaatctcat tatctttcaa aatctctgtt
     7621 gcagtacaaa ttagattatc taagtcaaaa cttcgagtta tctctagttg tttgttattt
     7681 acttctcttt ttagtatata agttttaaaa tacatttata tcaccccttg tatttactct
     7741 tttttctcta aaaatatatt gataagtaaa tatgcaacaa ataaaaaaat ctcaatgaca
     7801 catagtgtta tagctataac tagaagtgat tgatttttcc atgcactata agctatgatg
     7861 aaaagaaatg taaaagtaaa taatgtataa ttcataattt taactaagat atgacttgtt
     7921 ttcattgtta agtacaagtc tctttcatct gtactatcgt gtaattcttc aacaatgcct
     7981 tttcttgaaa aagcattaca aaaattaaag attgctatta caattaaaag aaatgatgaa
     8041 attaggaatc ttttatcttt taaaatgcaa taaataacta aactagcaca acataacaca
     8101 caaactataa tacccactat aaaacttcgt ttactcttta ctttcatgtt ttttatcctc
     8161 catttcttta ttttctttta aacaacatag ttcttcaatc gttgtaccaa acacttgtgc
     8221 catacgatag gcaagcataa gagaagggct atattgctct ttttcaatag aaataattgt
     8281 tctgtttgat acatggacta aatcagcgag ttgttgctgt gttagaccca tatttgttcg
     8341 aaattctcgt acttttgttt tcattccata acctctttct atatgaagct aacttcatgt
     8401 gaagttaact tcataatatc ctaaaatgag taaaatgtca atctcattag ataagtatta
     8461 taaaacctaa aagaataata tggtaaaaat tagatggaat tatttacctt aattctcttg
     8521 tttgtaaaac tttattgata tagaatttaa catatacata aatcggcaaa ggggcgcgaa
     8581 ttatgaaaat tagtaaatta ccagaagcgg aattaaaagt tatgagatat atttgtgaag
     8641 ctaacaaggt attaacttct agggcattag ttgctgcaat ggaagaaaaa tatgagtgga
     8701 atgaatcaac tacatttaca gttttgaaaa gattagagcg aagggagttt ttaagtacag
     8761 agaaaatagg caaacttaca cattataata tactagtaaa agagaaaaaa tatctaaggt
     8821 ttgaaacaaa ggaatttttg aagaacatac ataaaaattc aatttcaagt ttaatgtcag
     8881 cgttacatgg tgaagacgac gaagtggatg aagataaatt aagtgaactc gaagaatatt
     8941 ttaagaattt gaaataagaa aatgacaaat tgatacataa taaataaaat gaaggtatga
     9001 aattataact ttttctacct tcattttgtt tattattctg aacatgttaa gaaaatgaaa
     9061 taagtttgta ttttagttat ataaatttta atagaatatg tacagtatat agtagataga
     9121 atgtataaaa atagaattta aataagtagc tattattgca gtgaaatgaa tgtttctaag
     9181 tatatttggg tggaagaatg gtggaagaaa attagtgaaa tgtaattctt ccaccattct
     9241 tccacccaaa aaataaaaac tgttcaataa gaattaatat acatcaattc agctgaatgc
     9301 aatcaaaata cttaaatgaa agtaatctga aaacaacata attcaaatca atttattcaa
     9361 ataaacctca atttatctta aatcttaaga aatatttaag aattaggata atattttctt
     9421 aagatttata ttttattata tagttataag ggaaagttac aaaaatgtaa gccaaagtaa
     9481 tataacaatt tagtcattgt tgagaaaggg agtgaaagat attgtataat atagataagt
     9541 attattatac aatagaggaa gtgaaaatat gtacaatata ttagtagtag acgatgataa
     9601 agaaattgtt gattcgatag aaatatatct taaatcagaa ggatttaaaa tatataaagc
     9661 atatgatgga atgaaagctt tggaaataat aacagagaat gacattcatc taatactaat
     9721 ggatataatg atgcctaaat tagatggaat caaagcaact gtaaaaatac gagaagaaag
     9781 aaatattcca ataattttaa tttcagcaaa aagtgaagat acagataaaa taataggatt
     9841 aaatattgga gctgatgatt atataacaaa gccatttaat ctattagagc ttatagctag
     9901 ggttaaatca aacttaagac gatatgtaac tttaggtaat tatgaagata catgtaaaga
     9961 aattctaaaa agtggagctt tggagttaaa tacactaaca aaagaagtta aaattgatgg
    10021 tgaaaacata aaaatgactc caattgagta taaaataatt gaacttcttt tagaaaataa
    10081 aggacgagtt ttctcaatag atgagatata cgaaaaagta tggaatgagg aaagttttaa
    10141 ttcagaaaat acagtagcag tacatatcag acgaataaga gaaaagattg aaataaatcc
    10201 aaaagaacca agatttttaa aggtggtgtg gggagttggt tataagattg aaaaatttta
    10261 gtggaaaaat tataaaagaa aagagtttaa tatttaattg cattttgatt ttagtcattt
    10321 ttacatttac acaaatgtta ggaattttaa tatgttttaa ttttttctct gagagtttaa
    10381 tttactctat ggggattatg cttttcttaa actttatttt atataaaata ctgacaatgt
    10441 agatgaggtg ggcaacttgg agaaatatag agtattaaag ttagtttcat taatcataat
    10501 atctatctca tttccattaa ctatattgtt ggtgacaata ggacttgctg agcctaaata
    10561 caatatacaa aaaacttcta caaagagtgg gatttttgat atatctgatg atttttatga
    10621 tagtaatata tttgacgaag gaacaatggc aagtctatta cccaagttta agctatctgt
    10681 aggtggtaaa ttaactagta aagaaaaagg taaattccaa caagaaggta aaagtataga
    10741 tttaagtagt agtgattctg agtatgataa aatcatgatt aatgaacaag cttctgaaga
    10801 atatttaagt tatctaaaaa atataaaagt gtatgttatt aatacataca caggagtatt
    10861 ttatacaaat acgtcatatg ctagtttata tgattttaaa caaaaaacta agagctattg
    10921 taatgtagag ataataaata agataggaaa gagctcatat attaaaacaa taaatggaga
    10981 aaaatttgaa ctgaataatc tgagtctgga actaaatgaa gtagatgaag gttttgaggc
    11041 atatgtatct tttccagaag agccaactat agaagatgga atagtataca ctaattttca
    11101 aatatttaaa caagctacag aaaaagtaag attatttttt gtagctagtt taagtttagc
    11161 aattatatgt ttattatcta taattttata tatcaaaata aaaggtgaag ttttaaaaag
    11221 aaataatgta tttttactaa tatacagtaa gatacctatg gaatttaatg taatattagt
    11281 atctatttca attgcaattt taactatata tgctcttgct gtatcttatc atattgatgt
    11341 aattgcatta gtttctttat ctttactgat agtatcaaat aatatatttt ttataaatgt
    11401 gcaattgagc tatttagaaa ataaaatgtg tatatttaag acaagtatta tagtaagagt
    11461 gattttatgg ttaataaaaa ctgtaaggga ggtcagtaac gctataaaga aagtatcttt
    11521 agcaaggaaa gttattgtta tagtgataat atctttagct ttaatcatgg caattagcac
    11581 tcgaagtata ggtatgggat tactgttttc tatatgtatt tttttaggat ttacaatata
    11641 tataactaaa agactttcat atcttagtta cgtaatagaa ggtacagaaa gaataaaaaa
    11701 gggagaatta gattataaga taaaaattac tgggaatgat aattttacgt ctttagctga
    11761 aaatataaat aatattggag aaggtcttga tagagctata gaagagcaag taaaaagtga
    11821 aagaatgaag tctgacctta taacaaatgt atctcatgat ttgaaaactc cacttacttc
    11881 tataataaat tatgtagatt taattaaaaa agaagaatca atacaaccag agtatataaa
    11941 tgattatata aatgtacttg agtcaaagtc aaaaagacta aaattattga tagaagattt
    12001 atttgaagcg agtagagtaa gtagtggcaa tatagaatta caaattagca agattgattt
    12061 agtgcaacta ttaagacaaa gtataggtga acttgaagaa aaattatcta aaaataattt
    12121 atatttaaaa ttaaatgttc caaatgataa ggtgtatata tgggcagatg gaagaagaat
    12181 gtatagagta tttgaaaatc ttttatctaa tatagcaaaa tattccttag aagaaactag
    12241 agtctatatt gatgtatatg actatggtga aaatgtaaag gtaactatga aaaatatatc
    12301 ttcttatgaa ttaaattttg acccaagtga gattatggaa agatttaaaa gagctgatga
    12361 atcaaggaat actgaaggaa gtggattagg gcttgctatt gcaagagatt tagtagcttt
    12421 acaaggtgga aaattttata tagaaataga tggagattta tttaaagcaa atttagagtt
    12481 tcaaaaatat gaagaagaaa taacaaatat ggaatgagat agtatttaag tatatatatt
    12541 ttttttactg tcgaaagctt ttattataat atattagaat atttacagta gagaatgaag
    12601 agtaaaaaca gagtaacttt aagtcactct gttttttatt taaaatcaat aatataacaa
    12661 cttaaattaa aattgtacac atagttttaa aataacttat acaataaaac attatccaaa
    12721 tatcacaaga taattccaaa taaatatata gatatcactc ctgtaataat agaaatacca
    12781 atagatttaa acttatatgc aacaagaagt acaatcataa aagatataat tttagggtta
    12841 gctatagata aatatagact atctccatta aaaaatacat ctttagcagt taaggaagta
    12901 agtattgcta caggtataaa tttcatgatt tttttcaaaa aatcaggcat ctcttttttt
    12961 gaaaaaatta acataggaag cactcttggt agatatgtaa caatgtacat tccaattata
    13021 ataaaaaata tataattatt attcataaga ttcctccttt ttacagtaag attcttctat
    13081 aaagaaacca attaaagatg ctaaaattga tgcaactatt attactaaat tgcttttgaa
    13141 taatgcatat aaaataacag aaataatgat tgatgatata aatactacaa tataaatctt
    13201 attttttatt tgagaaacta ttaaagctat aaacatagaa gtcaatatga agccagaaat
    13261 agaactgtct atagatatac tttctcctaa aaatgctcca ataaaattag ctaaaactga
    13321 tgaaattaag cagaagaaat taagataaat agcatttcta tcagaccaat ttccagcagt
    13381 aaatttttca taattaactg caaaagtttc atctgttata gtatgacaaa atggcaatat
    13441 aaattttgga cttttatttt ttatgtacat gttcaaatta gaactcatta gaaaatgtct
    13501 taagttaact ataaatgtag tcaaaacaat agatgttaca cttgcaccag tagaaatcat
    13561 tgaagctgca atgaattgag aacttcctcc aaatactaaa gttaccatta gtgctatttg
    13621 aaaaggtgaa agaccagatt tttgtaatag tattcctcct actataccta taggtatata
    13681 acctaagaat ataggaattg aagcaataaa gctttctttt aattgttgag ttctttccac
    13741 aataaatcac tccttttgta ttaaatgtat taaagtattc gtaaaacaga aaaatagatt
    13801 aattgtataa ttgcaattat aattaaaaat aaatttggat taatatataa aatactatac
    13861 aacaaataga taatcttaaa cggttattaa attttatcta tttgttatta tgatgtaata
    13921 atacatatta atctctataa gttaattact atctggtgta attttatttt aacaacaaaa
    13981 aaaaatacac acaattaaaa tattgtgtgc aatacaatta ttttttctct tctaatagtc
    14041 tttttaatac gctcaaacca gctttaagtt cttctgtact ttctgtagca catacagcta
    14101 ttcttattgc agaaattggt ttttcttttc caacagcaaa acgttcagaa gcatagacct
    14161 gtacaccttc tttaagtgct tgtatctcaa atttttctgc cattattcca tcaggtagaa
    14221 tcaaccatct aaatatacat tcttcatttc ccaaaagatt atatccagat aaatattgat
    14281 taataatctt gtttcttctt cttgcagatt tacggcgtgc attaattaat ttatcggctt
    14341 cacctgatgt aatcatgcga taagcaattt ctgttaaaaa ataagattga gatagattaa
    14401 tgttgtataa tgcgcttgat aatgcctctc gatactgttt aggtgtagag atatatgcta
    14461 gccgtaaact aggtgcaata atttttgata aactcgtaat ataaattgtt tgattgggca
    14521 gataggaagc cactggattt aaatgtgttt cattaagtaa actatgaata gcatcctcaa
    14581 taacaattaa attgtattta gatgcaatat ttgcaatcat ttttcgtcca ttttgtgaca
    14641 ttatatgtgt agttggattt tgataatctg gtattatgta tagaccttta atattttcat
    14701 ttttacaagc atataatagt ccttcttcac taatctcatt atcttcttgt ttaattggaa
    14761 ttagttgaat cccaagcatt ttagctgctg tctttattcc aggataagtt aatgggtcta
    14821 cacctattct atctccatgt tgaaacaaac cagctaaaat agcaacaatt gcattttgtc
    14881 caccacttgc aggaagtaag ctatctgatg tagtttcaag cccagcttta taaattaatt
    14941 tggcaatagc ttctttttgc catttagcac catctctttg tccatattgg aatatggttt
    15001 taaaacctat ttccttaatt atgtgtttaa acaaagtatt catttcttct agcgtgaaat
    15061 ctggattcat tgtgcccatt tcaataaatg taattttatt attactggac atcaagaata
    15121 gatttgaacg tgtatcataa gatacaaaag ttccacttcc tgtaactcca cttattagac
    15181 cttttttttc acaaatttta aaagctctag agatagtact cacattaaca tctaagaaat
    15241 ctgccaattc acgttggggt ggtaatttag tacctggtaa taaaaatcca ttgttgatat
    15301 cttgttctag ttgttctgca agcgccttat ataatatttg cccttttgaa ggtctctttg
    15361 gtttccagtt cataggataa ttttcaaaag aattaattgg cataagtttt cacctcacat
    15421 tattaatttg attttttctt tatctaaaac aataaattct aaagatacaa ataaaaactg
    15481 ataggaaata attctatcag tttttgtctt ttaattgatt tcaaatcttc ctataatttt
    15541 tataccttct ttatcataaa gagggattgt ttttttaaat atattaaaat ttctttttct
    15601 tgtatataaa acagcttctt ctggtgtctt tggttgatta tcacaatcgt acaaatctct
    15661 tttttttacg tagccgactt ttttacctct acctattgtt tgtataagtt ctggttcaat
    15721 ggaatttcca tttgagtcta ttatatgtgt accataggtt tctccatttt tattataatt
    15781 aatcttacca atagtatatt ctacatcttc attttgttta aaattggagt tgatagaaaa
    15841 tattgaccct attattataa aggttaataa aaacttaaga cctaaattaa ttttcttttt
    15901 atcttgatta aacatagaat ttaacctctt ttttaaaata atattttttt tatttagaaa
    15961 ctgagatacg aatgatgaat acttcaaaga tttttgatac tttattgtat ctaatattat
    16021 atatgcatac tctttaattt cactcgtggt acacttgtca atcacaattt catcacaaga
    16081 taattcacat aattcatgaa aatatttttt taataagtat aaaattggat tgaaccaata
    16141 tatgatacaa attatactaa ttagaagctt aaataagtta tcttttcttt tataatgaat
    16201 taattcatgt ttaaagatga atcttaactc tttattattg tacttttttt ctggaattat
    16261 tattttacag cgtgttaaac ctataatggc aggagaataa atacctttaa ccttatatat
    16321 ctgaagtttt ttatttatac ttagttcttt tgcttgttca tctaagatac tagtaatatc
    16381 tatttctaaa atttcttttt taaaattttc tatgtaatta taaaatttaa aatttgttct
    16441 taaatagtaa aaagagattg ctataattcc taatatccag atgtacataa atatatctaa
    16501 agtaatgccc tggtcatatg gtatataatt atcgatatta tgcttataga tattaatagt
    16561 agtattattg atatttaata tatttaaatt tattttagat ataaacatca ttctgactac
    16621 tattaaaaga catataaaat aattaaactt aaaagtatat ttgcttatct ttttagtaaa
    16681 taaaatgaaa aaaagaatta gtggacttat gtaaatatta aatttaataa cttctataaa
    16741 taattccata aaatacgtct cctattttct tttttttagc cattgttcaa gttcttttat
    16801 gtcatcatct gatatttcat ttccacaacc taatgatgat ataaagctat gaatagaatt
    16861 tttatgtata agctctaaaa aatcctttgt ttcaaatttt agatattcct catgtttaac
    16921 tttaatagtg tataaagtat ttctaccatc tttttcaaat tctaaaaaac ctttatcact
    16981 taatctagaa agtactttac cagtagtacc ttttgtccat ccatgagaaa tttccatata
    17041 attagctact tctctggctg gtattttatc tatgccttta tcccatatga aattcataac
    17101 taacaactcg gattttggta tttttttcaa tataatcact cacctttaac ttgattatat
    17161 tagacaaatg tctaatagtc aatataatat atcatcatgt ataaataatc tttatattat
    17221 tttatgcttg agaactaagt agtatagatt tatagataaa aaagatattg agagttttat
    17281 ggaagagggg aatatatttt ctattagaga taagaatatg acatatattt tttattgatt
    17341 tgtacaaaaa tactatatag tgaataaatt aatatctttt ctaaattaga agaggagaat
    17401 cttattagat aaagattatc ctctttattg aaagtttaag ggatattaga gattattaat
    17461 ttttctctct ttttttaact aaaagcaata atacataaaa ttattattgc taatgatact
    17521 cttaatgtgg tatatagtca tagtgtcaca tgtttagcaa tagttttttc agtaattatt
    17581 gtaacgaata gtaggactca taaaatactt agaaatatgt ttttagataa attttaaata
    17641 tataatctaa cttatatcta taaattaaca ctattattct ccaattccat ttctagtttt
    17701 tttataaatt tattaaattc ttctggttgc ctttttccaa acaatactct tacaagagct
    17761 gaattgattt ttccaaatat attcctcttg ctctcacttt caataaaaat aagttttgta
    17821 ttttctaagt caatatgtac taattcatat ctagtaatag tactttctct tttattgctt
    17881 gtttttatct catatactct atttttttcg aaattagtta tttcaacctt aaatctttta
    17941 ttattttttt gctgtacaaa tgttcctact gcttttttct cattaaaatt aggaaaattt
    18001 agtcttgcag attttgtaaa tatattaaat atgtcttttg cagaatattt taataaaact
    18061 tcagttttaa atgtattcat atcgttttcc cttctgtata taaatttaat atttctaagt
    18121 taatttttaa gttaaaatag tataatatgc catattaaat catgttaaaa ctatttatat
    18181 agcta
//
LOCUS       DACXMZ010000065        18084 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.065, whole genome shotgun sequence.
ACCESSION   DACXMZ010000065 DACXMZ010000000
VERSION     DACXMZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18084
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.065"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            174..1604
                     /locus_tag="JD016_08665"
     CDS             174..1604
                     /locus_tag="JD016_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HAU4999709.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            1972..2550
                     /locus_tag="JD016_08670"
     CDS             1972..2550
                     /locus_tag="JD016_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator"
                     /protein_id="HAU4999710.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            2580..2726
                     /locus_tag="JD016_08675"
     CDS             2580..2726
                     /locus_tag="JD016_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HAU4999711.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            3031..4410
                     /gene="scfB"
                     /locus_tag="JD016_08680"
     CDS             3031..4410
                     /gene="scfB"
                     /locus_tag="JD016_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HAU4999712.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNSDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            4439..4936
                     /locus_tag="JD016_08685"
     CDS             4439..4936
                     /locus_tag="JD016_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAU4999713.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            5234..6898
                     /locus_tag="JD016_08690"
     CDS             5234..6898
                     /locus_tag="JD016_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAU4999714.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            6911..9361
                     /gene="recJ"
                     /locus_tag="JD016_08695"
     CDS             6911..9361
                     /gene="recJ"
                     /locus_tag="JD016_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAU4999715.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDDSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            9374..9886
                     /locus_tag="JD016_08700"
     CDS             9374..9886
                     /locus_tag="JD016_08700"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAU4999716.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            9960..12167
                     /locus_tag="JD016_08705"
     CDS             9960..12167
                     /locus_tag="JD016_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAU4999717.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            12192..12641
                     /locus_tag="JD016_08710"
     CDS             12192..12641
                     /locus_tag="JD016_08710"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAU4999718.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            12656..13270
                     /locus_tag="JD016_08715"
     CDS             12656..13270
                     /locus_tag="JD016_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAU4999719.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            13356..14915
                     /locus_tag="JD016_08720"
     CDS             13356..14915
                     /locus_tag="JD016_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HAU4999720.1"
                     /translation="MMLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLI
                     NRLIYENGVLFSSTEYYENGNLKYESIQNIENMNLKFLDGFDSSKTIEEFLNSLDSES
                     KRNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTI
                     LKDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLK
                     QFGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFN
                     ELDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVS
                     DLVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRA
                     SNLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAK
                     EGYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIK
                     RKKEEVYKNVD"
     gene            14905..16167
                     /locus_tag="JD016_08725"
     CDS             14905..16167
                     /locus_tag="JD016_08725"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAU4999721.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            16241..18028
                     /gene="aspS"
                     /locus_tag="JD016_08730"
     CDS             16241..18028
                     /gene="aspS"
                     /locus_tag="JD016_08730"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAU4999722.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         7366 a         1805 c         3376 g         5537 t
ORIGIN      
        1 aatgtccact attaacttta ttaccccaca tatataagat aaaattatta atataaaata
       61 taatctatcg acaatgaata gacttttaaa taaacttaat ataaaattaa ttaaataagt
      121 gcttaaaaac gatttatttt aatgatagaa tcatattaaa ggaggataat tttatggata
      181 taaagaataa aattaaatct atatttttac cagaaaaaaa tgactattta gatgatgaag
      241 aatttgagta tgatatagaa aataatgaag aagatttaaa ggaagataac aaagtacatt
      301 taaatgattt aagcagagta aaatatgatt acagagagtt aaaagacata gaaaaccaaa
      361 cagaagagat gtgtctgcta gcagtaaagc aggatggtac tataattgag tttgtaaagg
      421 acaagactta taaagtatgt atggaagcag taaaacagac atataaatct cttaagtata
      481 taacaaatca aaatgaggat atttgtatag aagcagtaaa acagaactac agagctttgt
      541 actacataaa taataagaca gaaaatgtac taattgaggc tataaaaaat gcatcaactt
      601 atgatgtcat ggaagtattt aaatttgtgg aagagcaaac agaagatgta tgtctagcat
      661 ttatagaaag ggcatctaaa aatgatgttg cagagatatt aaaaggaata aaagagcaaa
      721 ctccagccat atgtttagaa gctgtaaaaa aagatggaaa atctttagct tatgtaaagg
      781 aacaaagtaa tagtatatgt ttagaagctg taaaagaaaa ctacagtgca ctatcttgtg
      841 taaaagagca aactgaagaa atatgtatag aagcagtaaa acagaatgac tttgcattgt
      901 attatgtaaa tgaacagaca gaaaaaatat gtatggaagc agtaaaaaga agttatatgg
      961 ctctacagta tgtaaataag caaacagaag agatttgttt ggaagcagta agaattgatg
     1021 gaagagcatt acagtacgta aaagagcaaa cagaagaaat ttgcttggag tcagttagac
     1081 aaaatggaaa agtactgcaa tatgtaaaaa aacagactga aaatatatgt atagaagcag
     1141 taagaggttc atttgaagaa cttgaaataa aagaaatatt aagttatgta aagataccaa
     1201 cagaaagaat atttgtagaa gcagtaaagc aaaatggaaa aattcttaaa tatgtagaaa
     1261 atcagacaga attaatatgt ttagaggcag taagagaaaa ctataatgct ttagcttatg
     1321 taaaggaaca aacagaaaag atttgtttag aagcagttaa tcaaagttat gaagcgctta
     1381 aatatgtgaa agaacaaaca gaagaagttt gtctaaaagc agtaaaacaa gattatagaa
     1441 tgttgaaata tgtaaataat caaacagaaa aaatatgttt agaagcagta aagcaaaatt
     1501 atagagcttt agaatttgtt gataatcaaa cagaaaaagt ttgtcttgaa gcagtaaaac
     1561 agaatagaaa agcactacag tatgtaaaac aaaaacaaag ttaaaaggct aaaatatatg
     1621 gggaatatat gcaaataaat aaaatttgat attttgtaat aaataaaatg ggtatctcaa
     1681 aaggaacttt ttagtttgtg aggtactctt tttatatgaa atgaaatata tttttagctt
     1741 tttaacaatg gggaaaaggt ttatttctat tttgacatag gctttttatg catataaagc
     1801 ttgataaaat tgttttatta taattgtttt gttatataaa taataagaag aaaattatat
     1861 aggtcttttt ttgaccagtt ttacattttt aacaactagt tttgtaaagt atattgaatt
     1921 ttctcaaaaa agaatgtact ctatatatgt aaaatttttt tggaggtaaa aatgtttaaa
     1981 agatatgctg aaaaaatgac atctgtttta atttgtaata atatgataga taataatgaa
     2041 tctaaagtct attcttatgg atttgaaata ttaatagctt tcatagttaa tataaccacc
     2101 atgctgttta taggtttttt atttggtaaa tttacatatg tattattttt tttaatgtgt
     2161 tattgtccaa taagacaatt ttcaggaggg tatcatgcag acaattattt tagatgctta
     2221 ttaaccttta tttttataat tttatccaca atcttaatta tagaaaatat aaatatagac
     2281 ttatttaaaa atattattat gattattgca agtgttagtt gggttggaat ttgtgttctg
     2341 tgcccaatag aacataggag taaccctata agtgataggg aaaagttggt ttataagaaa
     2401 actgctatat ttatatctac agtagtgtta ttgataacaa ttttaagtct ttctttgagt
     2461 atatttgtag attatttcac atatagcgca tttgccatgt tttggatttt tgtgatgtta
     2521 gtattaggta agttaaaagc taaagtataa gtaaaatatg tatgagggag agttaagtta
     2581 tgaaaaagtt tattgtaaga tttatgaagt ttgctagctc attggcactt agcactgcta
     2641 ttttgtcagc taatagtacc tgtccttgga ttattcatca gcctaaagta cccaaagaaa
     2701 tcagcaatct aaagaagaca aattaaatac aagcacttat ttatacaaaa ttatcaggaa
     2761 aaaagaaatt attatatttt aataagatta aaacaatata aaatcagata caaaatttat
     2821 tttatttatt ttataggagc ctaataccta tttggtaata tattagtgcg ccaatttagg
     2881 gggatatagt gtatttaatg aaatgactta cggagagtaa gtcatttttg tattgtatgt
     2941 attttatgta aacttatgat ataatgaagg gtaaagttta attaaaataa taaaaaactt
     3001 tagtatagat agtaatttag gaggtagggt atgagtatga tacataaatt ttctatgaat
     3061 ggatataata tcgttttaga tgtgaatggt ggagctgttc atgtgttaga tgatgttgca
     3121 tacgatttgt tagattttta taaagaaaaa agtaaagaag aaatattaga gatattaaaa
     3181 tcaaaatatc aagaagaaaa aataaatgaa gcatatgaag aaatattaaa tttggaaaaa
     3241 gagggtcttt tatatacaga agatacatat caatatcatc caagctttgt acatagagag
     3301 cctgtagtaa aagctttatg tttaaatgta gctcatgatt gtaacttaaa atgtaaatac
     3361 tgttttgcag ctcaaggtga ttttggagga gaaaaagaac ttatgagttt tgaagttggt
     3421 aaggcagcca ttgactattt gattgctaat tcaggaagta gaaagaacct agagatagat
     3481 ttctttggtg gagagccttt gatgaacttt gaagttgtaa aacaactggt tgactatgga
     3541 agaagtgtag aaaaagatta taataaaaat ataagattta caataactac aaatggtgta
     3601 ctgttaaatg atgagattat agattatata aatgaaaata tgcataatgt agttttaagt
     3661 ttagatggaa gaaaagaagt taatgataat atgagaccaa ctttaaatga taaaggaagt
     3721 tatgatatta cattaccacg atttaagaaa cttgtggaaa aaagagcaaa agataagtat
     3781 tattatataa gaggtacttt tacaagagat aatttagatt tttcaaaaga tgttatgcat
     3841 tttgcagatt taggatttaa attaacctct gtagagccag tcgttggaga tgagagtaat
     3901 ccatatgcac ttagagaaga agatttacct aagatatttg aagagtacga aaaatttgct
     3961 gtagagtatg cagatagaca attacaagga gatggattta aatttttcca ttttatgata
     4021 gacctaaatc aaggtccatg tgtaataaag agaataactg gatgtggagc tggaaatgag
     4081 tatctttctg taactccaaa tggagatatc tatccatgtc atcaatttgt tggaaatgaa
     4141 gaatttaaaa tggcaaatat atttgatgaa gaaatagtac ttccagaaaa tttaaaaaat
     4201 atgtttagag aagctcatgt atatactaaa gaagagtgta aacaatgttg gaataagttt
     4261 tactgttcag gtggatgtca tgcaaatgcg ataaacttta atagtgacat atcaaaacca
     4321 tatgaattag gctgtgaaat gcaaagaaaa cgtacagagt gctctataat gatacaagct
     4381 aagttaatgt tagaaggagc aaccaactaa atttatattt taaaggagtt gtttatatat
     4441 gattagagat tatttagaag ataaaccttt aattgacgaa agtgtatttg tggcaaaatc
     4501 agcagatgta attggaaatg ttaaaatagg caaagattca agtatatggt acaatgcagt
     4561 agttaggggt gatgaaggac caataactat tggagaaaac actaatatac aagattgttc
     4621 tatagtgcat ggagatacag aaactataat tggtaacaat gtaacagttg ggcataggtc
     4681 tatagttcat ggttgtaaaa ttagtgataa tgtgttaatt ggtatgggtt caataatatt
     4741 ggataatgct gaaataggag aatatactct gataggagca ggtacactaa ttacatctaa
     4801 taagaagttt ccaccaggtg ttttgataat gggttctcct ggtaaggttg ttagagagct
     4861 tacagaagaa gataaaaaat atattgatga atcttatgaa tggtatttag aggcagcaca
     4921 aaatcaaaaa tattagttta aagagtggtt gtttcaaaac atatttaaat ctgttttgag
     4981 gcaaccacta atttatttta ttagattttg ttgatatttt agttttcaaa aagtatttga
     5041 ttaaaccaga tataaaatgt caaaaataaa aattaactga gttaaataat tttaataaaa
     5101 ttggtagtat atgtgataaa attaaatatt aaagaggata taggatatta gaatatcaat
     5161 tggtgaaata atgtcatata cgataaatta aggtgtaagt ttttgggtat acctatctta
     5221 ggagtgaaaa gatttgagaa taagttatag aaatttaaag cgatatagag aaattggata
     5281 tgttttaata aagtatggtt ttagttttat tgtagaaaga ctaaatatag aaggaattgc
     5341 ctataaaata ccactatttg acccaccaga agaaataaaa aatatgacaa ctggtgagag
     5401 aatgaaaaga gttttagaag aactaggacc tacatatata aaaataggac agatattgag
     5461 tacaagaaaa gacttgttag accaggatat aattgatgaa atatcaaaac tcagagatga
     5521 tgtagagaaa tttgattcaa atatagctat tgatatattt aaagaagaag tgggtttaag
     5581 tatagaagaa atattcttag aatttaaaga agaaccaata gcagcagctt caattggtca
     5641 agtttatgaa ggtgtactta aaacgggaga agaagtaatc gtaaaaattc aaagaccaaa
     5701 tatagagaaa ataataaaat ctgatttaga gattcttaga actattgcaa atactttaaa
     5761 agatttaaaa aaagatttta atttagattt agtacagatg attgaagaat ttcaaactca
     5821 attaatgagg gaacttgatt atacatttga agctataaat gcaactaaat tctctaggat
     5881 atttaaaaat agtgatgaag tatatatacc taaagtatat tcagaatata atactaagaa
     5941 aattttagta atggagaaag ttaatggtac aaagttaagt gatgtagaaa aaataaggag
     6001 actaggttat aacacaaaaa ctattgttga aataggcgtt aggtcatttt ttacacaggt
     6061 attatctcat ggattttttc atgcagaccc acatccaggt aatatattcg tagttgctaa
     6121 aaataagata gcatatatag attttggaat gattggaatt attgataata aaactttaaa
     6181 ccaattaaat gaaattgcat tagcaggtgt agaaaagaat gttgacaaga taatatatct
     6241 attgatagaa atggatgcat taaatggaga ggctgatatt aagggactta gacaggattt
     6301 actttatcta atacattatt actatgatat ttctatagaa aaaataaatg taacagatat
     6361 attaaatgaa ctatttaggt tttttagaca gtataaaata gtcatgcctg cacaatttgt
     6421 aactcttgca aagacagtta taacattaga aggaacaagt agaactttaa acacagattt
     6481 ttcttttggt tctatgggaa aagagtttat gaaacatcat tataagagta aatttaaccc
     6541 taaaaatgtt gttttaagtt caagacaaaa tgtagaagaa atattactgg acataaaaac
     6601 tattccaaag caattaaaag caattttaaa aaatatagaa agaaataata taaaaatgca
     6661 aattgaagat gttaaaatga cgaggttaga aaattgtata atagagctaa catctcaaat
     6721 atctttgagt ttagtgttgg catctattat agtaggttct tctcttataa ttgcatctcc
     6781 aaatatagaa aataatatat ggataaaatt tactgcaata gcgggtttct ttatatcctt
     6841 tataataggc ctttgcttag ttataagaag tattaggtca aaatacaaaa aagattaggg
     6901 ggaattaaat ttgaaaaaat ggatattgaa gtataaaggt aagatagagg aaagtgaatt
     6961 tagcaaaaaa cttaatattt ccccggaaat ctgccaaatt ttaaaaaata gagggatatg
     7021 tactgaaaaa gattcagaaa tatttatgaa tccatcttta gattatttaa gagacccttt
     7081 tttaatgaaa gatatgcaaa aagctgttga tagaataaag ttagcagttg agaagaacga
     7141 aaaaatttgg atttacggag attatgatgt tgatggtgta tcatctacat ctatcttgtg
     7201 cttatatttt aagagtatag gttacaatgt tgattactat attcctaata gattagaaga
     7261 agggtatggt ataaatgaag atgctattaa gcttataagc tctaggggat gtgacttgat
     7321 tataagtgtt gactgtggaa ttacatcagt gtctgaggtt aatacagcga atgatttggg
     7381 catagatgtt ataattacag accatcatga atgtcaaagt gaaattccat cagcctttgc
     7441 agtaataaat ccaaaacaag aagattgtaa ttatccattt gattcactat gtggatgtgg
     7501 tgtagcattt aaaatgattc aagccttgac tccaaaagaa gaatttaaaa ctagtatgta
     7561 taattattta gaaatagtaa cactagcaac tatttgtgat attgttccac ttatagatga
     7621 aaatagaata attgtaaaaa atggtttaaa atcaatgaaa gaaggcaaaa atattggatt
     7681 aagggagctt ataaaagttt gtggtgtaga aagtgataag ataggttctt ctcatatagg
     7741 ttttgcaata ggtcctagga taaatgcttc aggtcgattg ggatattctt acttaggagt
     7801 agagctattt actactcaat cacaagaaga ggcagtagaa atagctagta tattggaaga
     7861 aaaaaataat gaaagacaga tgatagaagc taagatgtat catgaagctg aagaaatgct
     7921 aaagtctaat agcagataca atgatgacaa agtgttggtt ttagctaaag agggttggca
     7981 acatggaata ataggaattg tagcatcaaa gttaactgaa aaatactaca aaccaacaat
     8041 tcttctaggt atagaaaatg gggaggctac tggttcagcg aggtcaataa aaggatttaa
     8101 tatatttgaa gctctcataa agtgcaaaga cttgatgact aagtttggtg gtcatgaaca
     8161 agcagcaggg ttatccttgg attcagacaa tgtagaaatt ttagcaaatg aaataaataa
     8221 atttgcagat tataatttaa ctgaagatga tatgatagag aatgtaaatg tcgagtttga
     8281 attacaagaa aatgtaataa atttaaatct tgtagaagaa ttacataagt tagaaccctt
     8341 tggattaaat aatcctaatc ctagatttat agttagaaat tatattttaa aagatttgaa
     8401 agttataggt aagaatcaac aacatctgaa attaagtata gaaaaagaga aaagctatga
     8461 atgtatagga tttaatatga gtcatttaaa atcaatgtat aaagtaggag acaaagtaga
     8521 tgtactattc caattagatg aaaataacta tatgggaaat aggaaagtac aatttttact
     8581 taaagatata cgattggctc gacctaaaag tgcaagtaat gacaaacttt cattgaaatt
     8641 aatgtcaaaa ataataccaa aagatactca aagcctgtat aatatatctg tgtctgattt
     8701 tgaattattt gatggaaata cagatataaa tatatttgat tattttgaaa aagatacact
     8761 aataatatct aattctataa atggatttta tagagcaatg tcagatatat cacttataga
     8821 tttggatttt aacataaatt acaatattat tgaagatgat agcaaaaata ctgataaatt
     8881 agagcttata ttttctccta atattgataa aatagactta aaaagatata ataatattat
     8941 tctttatgat tatttataca ataaaggcga atattcgtat atatatgaga ataaaagaga
     9001 agaaagtgaa attataaaat attataataa aacagaccta ttatatttaa agaatgtagt
     9061 tagtaatata gttccatcaa gagatgaatt tataacaatt tataaacaag cactcataaa
     9121 aaaagaaatt gatttggata tggtaaatat aagggaaact tttaatgtaa ttccattaaa
     9181 gttttttacg atattaaatg tatttagaga attaaattta cttgacttta atttaaatta
     9241 tgaaaaaaat tctgtactca taagaatatt gccaaaacca cagaaaaaat tagacttaaa
     9301 tgagagttta atactcaata atctaaaaaa tttagaaaaa caatacaata gcagttatta
     9361 ggaggcgttt cccatggatt taaaaaactt tataaggaat attgatgatt tcccaaagcc
     9421 aggcatagat tttaaagatg tgacaacttt atttaaagat ggagatgcat ttaaatatgc
     9481 agtggattct atagttgaag aattgaaaga taaagatgta gatttagtaa taggaccaga
     9541 agcaagagga tttttgatgg gtacacctgt agcatatgct ttaggagtag gatttgttcc
     9601 aattagaaaa cctggaaagt taccaggtga agttgagagt tatgagtatg gattagagta
     9661 tggtacagat actttagaga tacataagga tgcaattaaa aagggtcaaa aagtagcaat
     9721 agtagatgat ttattggcta ctggtggaac tatggaagcc gctgccaagc ttgtagaaaa
     9781 attaggtgga gaagtagttt ctatgcaatt tttgattgag ctaaaattct taaatgggag
     9841 agaaaagttg tctaactatg atgttaattc tcttataaaa tactagtact tttataatag
     9901 ttggtataac accaactatt ttcatatata ttaaaatttt atacaaaata ggtggttata
     9961 tgcatgataa agaattacaa gaaataattg aaaaaataaa agtttatgca cctaatgcaa
    10021 attttgaaat ggttgaaaaa gcctataatc ttgcaaaaaa agcgcatgaa ggacaatata
    10081 gaaaatctgg ggaaccatac tttattcatc cattagcagt tgcaaatatt ttaatagaga
    10141 tggaattaga tattgaaact ataacagcag gtttacttca tgatgttgta gaagatacag
    10201 aatataccta tgaagacata aaaaaagaat ttggcaaaga agtagcggat ttggttgatg
    10261 gagtaactaa acttggtcaa ataaaatatc gttcgaaaga agaaactcaa tctgaaaatt
    10321 taagaaaaat gtttttggca atggcgaagg atataagggt tatacttata aaacttgctg
    10381 atagactgca caacatgaga acattaaaat acatgcctcc agaaaaagca aagtataaag
    10441 caaaagagac acttgaaatt tatggtggta tagctcatag attaggaatt tccaagataa
    10501 agtgggaact agaagacaga gcattaagat ttatggaccc agaagggtat tatgatttag
    10561 ttagtagagt atcaatgaag agaagtcaaa gagaggatta tattcaagga attgttgaac
    10621 ttcttaaaga taagtttaaa gatttaagta taccatgtga tgtatatggt agaccaaagc
    10681 atttttacag tatatataga aagatgcaaa aaaaacataa gacttttgaa gagatttttg
    10741 atttaacagc agtaaggata ttggttgata atgtaaagga ttgctatgca gtacttggta
    10801 tagttcatac attatggaga cctattccag gaagatttaa agattatata gcaatgccaa
    10861 aacccaatat gtatcaatca ttacacacca ctgttgtagg tcctgatgga gaacctttag
    10921 agatacaaat aagaactcat gagatgcata atatagctga aaatggtata gcagcccatt
    10981 ggaaatataa agaaggtatc tctagcaaag agtcaaaggt tgaagaaaaa cttcaatggt
    11041 tgagacagat gatggagtgg gaaaaagatt taaaagaccc acaagagttt atggatgcac
    11101 ttaaagaaga tgtatttaat agtcaagttt atgtatttac gccaaaagga gacgtaattg
    11161 aattaccagc agaatcaaca cctatagatt ttgcctatag agttcataca aatgtaggta
    11221 ataaatgtgt aggagctaaa attgatggaa gaatagttcc tattgattat aagcttcaaa
    11281 atggtaatat tgtcgaggta cttacatcat ctaattctaa tggaccaagt agggattggc
    11341 ttaatatagt gaagacccca aatgcaaaaa gtagaattag acaatttttc aagaaggaaa
    11401 gaagagaaga aaatgttgac cgtggaaata caatattaga aagagaattt aagaagcatg
    11461 atataccaca aaaagaccct cttgttgaaa aatatatggc attagttgct aagaaattta
    11521 atcagccaag tgttgaggac ttaattgcca ctgtaggtta tggtggtata atgtcatctc
    11581 aagtggtatc taaggtaaaa gacctatatt taaaagaggt taaaaaagct aaaaaggaac
    11641 aacagataca agaagaagat ttaaataaac acaatataag tgaaacagaa tacagtaaaa
    11701 agagaaagaa aaacgcatca caaggaatta tagtaaaagg gcttgacaat atcttagtaa
    11761 gatttgctaa gtgttgtaat cctcttccag gagatgaaat tgtaggatat attactaagg
    11821 gtaggggagt agccatacac agagctgatt gcccaaatgc aaacaataat ggtgatttct
    11881 tccaaaatag attggtggag gtttcatgga gttcagcagg aaaaggtaag tttgaagcgg
    11941 aaattcaaat aaaagctatt gacagaagag gaattgttaa tgaaataact catattgttg
    12001 caatggataa acttagttta aacggaataa atgcaagaaa aggtaaggat agtattgtaa
    12061 gtataaactt acttgtagag gttaatgaca cagaggaatt aaaaattcta atgaaaaaat
    12121 taaaagcaat tcctggtgta gaaagtattt atagaatgac aaattagagt taaaataata
    12181 gggaggaaac tatgagagca gtagtgcaaa gagtatcttc atctaaagtt acagtagatg
    12241 aaaacactat aggtcaaata aataaggggt tactagtatt gttaggagtg actcatgatg
    12301 ataaatcaag tgatgtagat tatatgattg ataaaatatt gaatctgaga atttttgaag
    12361 atgaaaatga caagatgaat ttatctctaa tggatatagg tggagagctt ttagtagtat
    12421 ctcaatttac tttatatgga gattgcagaa aaggaagaag acctggattt agtaatgcag
    12481 ctagaccaga acttgcaaat aacctttatg aagaatttgt aaaaaaagca aaagataaag
    12541 gtgtgacagt tggtacaggt caatttgcag ctcatatgat ggtagagtta actaatgatg
    12601 gtccagtaac aatactatta gactcaagta aatcttttta agggggaaaa ctttaatgat
    12661 tataaaaaaa tttgtagata gttattttgg agtaaatact tatgtattag gtgatgaaaa
    12721 gactaaaaaa tgtgcagtta tagacccagg tggaagttta gtagagacat taagttttgt
    12781 aaaagaaaat gaattaaata ttgaatatat tatccttact catgggcatg gagaccatat
    12841 aggatatgtt aaggatatta aagaaaaaac tggtgctaag gtagttgcac atgttgatga
    12901 aaaagaatta ttaaatgata aaaataaaaa tctaagttat acaatgagat gtggagcaca
    12961 agagtttgat gcagatatct atgtaaatga taaagacaag cttgaacttg gtgaattaaa
    13021 aatgactttc ttgcatactc caggacatac tcaaggatgt atgtgtataa gagttgaaga
    13081 tgaaatgttc acaggagata ctttgtttgc tggaagtatt ggtagaactg atttatatag
    13141 tggggacttt aaccaaatgg aaaaatcact taagaaatta tgcaaatatg aagatgctgt
    13201 tagagtatat cctggacatg gaccaaccag tactctaggg gttgaaaaga agacaaatcc
    13261 ttacatgtaa ttttatatta gtatttaaaa aagtataaaa tatttaaact atattagtga
    13321 gggggttctt ctactgttaa atagaaggag ttaagttgat gttgtatgtt tttttaaaag
    13381 gacatgatta taagtatgaa gtagcagaac ttataaaatt gtttaccagt gaatttaagt
    13441 ttaaagactc taatgctaac aataatatag agtcaaagca tttaataaat agattgattt
    13501 atgagaatgg tgtattattc tcaagtactg aatactatga aaatggaaac ttaaagtacg
    13561 agtcaataca gaatatagaa aatatgaatc ttaaattttt agatggattt gatagcagca
    13621 aaacgattga agaatttctt aatagcctag atagtgaaag taagaggaac tttaaaaagc
    13681 tgtgtaaaga aaatatcaaa aaaagcatgt ttgtagtgct taaacaagta tttaattctt
    13741 atgtaccatg gggaattttg actggtatta gaccagtcaa aattgtccac aatttaatgg
    13801 ataaaaagtt agatgataat agcataagaa ccattctaaa agataattat tttataatag
    13861 atgagaaaat agatttagct cttgaaatag ctaaaagaga gcgattattt atgtatccta
    13921 tagataaaaa taaaatttct ctttatgtaa gtattccatt ttgtccaact agatgtgtat
    13981 attgttcatt tccttcaaat tcattaaaac aatttggaca tttaaaaaga gaatatgttt
    14041 ataaattgat tgaagaaatt aaggggtttg ccaaagttat taaggagact aagaaagaag
    14101 ttgaaactct atatattggt ggtggaactc caactacact tgatgaggaa gaattggatt
    14161 tgcttataaa ttcattgttc aatgaattag atttaagtaa aattaaagaa tttactgtag
    14221 aagcaggtag acctgacact attacagaac aaaaactaag agtgttaaaa aaacacaatg
    14281 ttagtagaat tagtataaat cctcaaacta tgaatgatga taccttggta aagattggaa
    14341 gagagcatag agtatctgat ttagtagatt gctttaatat ggctcgtaga atgggatttg
    14401 ataatataaa tatggatata attttaggtc ttatagatga agatttaaat atggtaaaga
    14461 atacattgga ggagataaaa aaactttctc ctgaaagcct tacagttcat acattagcca
    14521 taaagagagc atccaactta aatataaata tggataggta taaagaacat ctaacacaat
    14581 atgaagaaat ggtaaaaatg atagacttat caatgaaata tgcaaaagat atgggattaa
    14641 atccatatta tatgtacaga caaaaacata tgttgggaaa tttagaaaat ataggttatg
    14701 ctaaagaagg atatgagtgc atttataata tacagattat ggaagaaaaa caaagtaact
    14761 atgcattagg tgcaggtgct atatcgaaat ttgtttatgt agatgaagat agaattgaaa
    14821 gagtagaaaa tgtaaaaaat gttgaacaat acattgaaag agtagatgaa atgataaaaa
    14881 ggaagaaaga agaggtatat aaaaatgttg actaaagctc ctagaggaac taaagacata
    14941 actccaaaag aggcttacaa atggcgttat gtagaaaata aatttagaga aatatgtgct
    15001 ttatatggat atgaagaaat ggtaacacct atatttgaac atacagaact tttcaagaga
    15061 agtgttggag atactacaga tatagttcaa aaagaaatgt attcttttaa agataaaggt
    15121 gatagagaaa ttaccctaaa gccagaaggt acagcaggtg tagtaagagc atttatagaa
    15181 aataaattat atgcagatac tcaaccaaca aaattatttt atgtgacacc ttgttttaga
    15241 tatgaaagac cacaagctgg tagacaaaga caatttcacc aatttggaat agaagcactt
    15301 ggaagtgata ctccttcaat ggatgctgaa ataatcgcac tagctgttca attttttaat
    15361 gaagttggat taaatgattt ggtagtgagt ataaattcag ttggctgtcc agtttgtaga
    15421 aaagagtaca atgctttatt aaaagagtat cttgactcaa aagctgatat attgtgtgat
    15481 acttgcaatg agagaagaga aaaaaatcca atgagggtta tagactgtaa aaatcctaca
    15541 tgtaaagaaa atatcaaaga tataccattt attgcagacc atttgtgtga tgattgtaaa
    15601 tctcactttg ataaacttca agaatactta aaggaaatga atataaactt tgtaattgat
    15661 aaaacaatag ttagaggctt agattactat agaaaaactg cttttgaaat tatatctaat
    15721 gatataggag ctcaaagtac tgtatgtggt ggaggaagat atgatggtct tgttgaacaa
    15781 ttaggaggac caaaaggtat aagtggaatt gggtttggac taggaattga aagactttta
    15841 cttacacttg aaggtaatgg aattgaaata gaaaaccctc aatctacgga tatatttata
    15901 gtaactatag gtgaagaagc aaatacaaga agctttaaat tgttaaaaga cttgagacaa
    15961 aatcatataa gtgcagataa agaccatata gagagaagtg ttaaagcaca atttaaatat
    16021 tcagataaaa taaattcaaa atttacaata gtaattggag atgatgaact taaaaatgac
    16081 actgcgacat taaaaaatat gaaaacatca gagcaaacta ctgtaaaatt aagtacatta
    16141 gttgaagaat taaagcaaaa gctgtaaaat aagaaagtat gatgttttgt gtattagata
    16201 aatatatagc attaaaatta aataaaatgg aggtagtatc ttggaaactt taaagggttt
    16261 aaagagaact cactactgtg gagagttgag agaaaaaaac ataaatgaag aagttgtact
    16321 tatgggatgg gtacagaaaa aaagaaattt aggtggtctt gtatttgttg atttaagaga
    16381 tacaagtgga ttatgtcaaa tagtttttga tacagatgta gataaagaag cttttgaaaa
    16441 agctgaaaaa ttaggggctg aatttgtaat agctgtaaaa ggaaaagtat gcgaaagaca
    16501 atctaaaaac cctaatatgc caactggtga tatagaaata tttgcaactg aacttagact
    16561 tttaaacaag tcagaaactc cgcctattta tataaaagat gatgatgatg tttctgaagc
    16621 actaagatta aaatatagat acttagattt aagaaaacca tctatgcaaa gaaatttaaa
    16681 attaagacat aaagtaatga atattacaag aaattactta tcaaacaata gattttgcga
    16741 aatagaaaca ccatttttaa tagctccaac accagaagga gcaagagatt atctagtacc
    16801 aagtagagtt aatccaggaa aattttacgc attaccacaa tcaccacaat tatataaaca
    16861 attattgatg gtaagtggta tggatagata tttccaaata gttaaatgct ttagagatga
    16921 agacttaaga gcagatagac aaccagagtt tacacaaata gactgcgaaa tgtcttttgt
    16981 tgagcaagaa gatgttatga gtatgattga aggtttgctt gaagcgatat ttaaagaagt
    17041 tttagatgta gaattggcac ttccacttcc taagatgact tatgcagaag ctatgtctaa
    17101 atatggttca gataaaccag atactagatt tggatatgag ttaacagata tatctgatgt
    17161 tgtttgtaac tgtggattta aagtgtttgc tgatgctaca caaccaggaa aaagtgtgag
    17221 aggtataaac gtaaaaggca aagctgacga ctttactaga aaacaaatat catcactaga
    17281 agaacatgct aagacatata gagcaaaagg tcttgcttgg atgaaagtag gacaagaagg
    17341 agtaacttct ccaatagcaa aattcttcaa tgaagaagaa atgaacgcga tacttacaag
    17401 aatgaatgct gaagtaggag atttactttt atttgtagct gataaaaact caatagtatt
    17461 tgatgcttta ggacaagtta gacttgaagt agcaaatagg cttaatctat tggataaaaa
    17521 tgtatataac ttactatggg taactgagtt cccagttttt gaagaagatg aagaaacagg
    17581 cacattctca gcaatgcatc acccatttac atcacctatg gatgaagatt tagacaagtt
    17641 agaagaaggt gataagtcat ctttaagagc gaaagcttat gatattgtat tgaatggtta
    17701 tgaaataggt ggaggaagtg ttagaatatc aaattctgat gttcaatcaa gaatgtttaa
    17761 agcacttggt tttacagagg aaagagctaa tgaaaagttt ggttatttat tagaagcatt
    17821 taaatatggt actcctcctc atgctggtct agcttttggt cttgatagac ttgttatgtt
    17881 acttgcagga actgataata taagagaagt tattgcattc cctaaaaatc aaaatgctgt
    17941 ttgccctatg acaaatgctc ctacattggc ggaagatgag caattagaag aattaagtat
    18001 aaaagttgat ataaaagata atgaataata tgtagttgca agaaactaaa atgtaaaaag
    18061 tctggatata aagtatattt atat
//
LOCUS       DACXMZ010000066        17935 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.066, whole genome shotgun sequence.
ACCESSION   DACXMZ010000066 DACXMZ010000000
VERSION     DACXMZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.066"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            203..1711
                     /locus_tag="JD016_08735"
     CDS             203..1711
                     /locus_tag="JD016_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HAU4999723.1"
                     /translation="MLKRVRCSYLKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRV
                     LEYETNDIITVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRK
                     FELDVTNLLKKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDIN
                     ETFARVSADNFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSD
                     RASLAKSSIKNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIK
                     LDKFCGSEALVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKG
                     ISPLPISVNFSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVS
                     RNLQDIGFIVAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVS
                     LIKQLGIRVVAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1744..5181
                     /locus_tag="JD016_08740"
     CDS             1744..5181
                     /locus_tag="JD016_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAU4999724.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLITYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVELGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            5199..5336
                     /locus_tag="JD016_08745"
                     /pseudo
     CDS             5199..5336
                     /locus_tag="JD016_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088348.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            5628..8681
                     /locus_tag="JD016_08750"
     CDS             5628..8681
                     /locus_tag="JD016_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HAU4999725.1"
                     /translation="MKKMLKRLCTGFLALATVVTALPTTPVHAESKQYWTESKERVGI
                     VEKVMNDGSIGSTFNEGHLTVESEDAYCIDINTDFKNGYKTRADASTRMSADQISDVA
                     LSIEYVKQYTDSHSGISNNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFSGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFADKDCTKQLATLTTDENGNTDVAEVKAGTVYIKELSAPAGYKVDKK
                     VYPLTIKAGETATLNVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKAPNGYLLEGAYMQAGDKTEQIKGLYVTQITEDGDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGIASTSADLLPYGNFRIVESEAPNGYLTDGAKPIDFAITENGKIVDLTDEARSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGENHVVVTDDNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELLIDGKRVENDYTFVADEEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKEVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKPEVPEEPEKPEIPPIPE
                     KPSKPSDSPKTGDSTNVMAFIVMLLTSAGGLAGTYLYKRRKMKKS"
     gene            8694..8896
                     /locus_tag="JD016_08755"
                     /pseudo
     CDS             8694..8896
                     /locus_tag="JD016_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8918..9244
                     /locus_tag="JD016_08760"
     CDS             8918..9244
                     /locus_tag="JD016_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 domain-containing protein"
                     /protein_id="HAU4999726.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESDHVFDRDKDNRRFFARRSYNL
                     YSDVQKGENVVVEIPVQAGEKKFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            9260..9643
                     /locus_tag="JD016_08765"
     CDS             9260..9643
                     /locus_tag="JD016_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 domain-containing protein"
                     /protein_id="HAU4999727.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVMELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNAGSPQNSTPQGQPKK"
     gene            9738..10280
                     /locus_tag="JD016_08770"
     CDS             9738..10280
                     /locus_tag="JD016_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999728.1"
                     /translation="MKYLGTILVAYIVLFILDIVQKRNHKTTKSLNEFSIRTISDVSF
                     ICAIGALFLLGVLIWGFWSEPENFYSKRYLMIIIPFSIIWFGVTLFGMIAPLKGVWEI
                     KVEGDNITVIKMFIFRRYWKISDISYCKMKRGGINVYVNGRKKKAFFVDGMTDHFDNF
                     IKRIEKEGKEIIIPEPKDTN"
     gene            10390..11763
                     /locus_tag="JD016_08775"
     CDS             10390..11763
                     /locus_tag="JD016_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAU4999729.1"
                     /translation="MQKIFNKGHRIRASDKHLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLKNGLTLSPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLAKM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGSLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGRLAQEKQ
                     VMPEQQE"
     gene            11776..12174
                     /locus_tag="JD016_08780"
     CDS             11776..12174
                     /locus_tag="JD016_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU4999730.1"
                     /translation="MEYLRTTTYNRITGMNMSGVCINYDNFWNDVRMTADCEFDKDEY
                     SPTERYHNRLVEIMKNVWSGKKRLPVVFSIRLEKYISLVDYPVCYTFAVIDKGFFKRT
                     YRKEEIPEEILKECIAKDNDCIVFYVGMNR"
     gene            12406..13602
                     /locus_tag="JD016_08785"
     CDS             12406..13602
                     /locus_tag="JD016_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAU4999731.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNVNYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LVDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPT
                     KDLVES"
     gene            13615..13749
                     /locus_tag="JD016_08790"
     CDS             13615..13749
                     /locus_tag="JD016_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAU4999732.1"
                     /translation="MWVLLKDFLLVSMGMGIGVVLMCILNVGKEADYEMKQLEESEDN
                     "
     gene            13750..13971
                     /locus_tag="JD016_08795"
     CDS             13750..13971
                     /locus_tag="JD016_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999733.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            14050..14556
                     /locus_tag="JD016_08800"
     CDS             14050..14556
                     /locus_tag="JD016_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU4999734.1"
                     /translation="MIIYSKLEINRIKDFWELLNRLDTETDYMMYEPNERKAKTTIQE
                     LKLDIQDNVIQGFDFLQVATENDKIVGYIRAERGKFERNFHTAYIVIGILKKYRGKGI
                     GTTFFNNLDLWAKTNNIVRLELTVECCNNTAKNLYEKNGFKIEGIRKKSMFVAGSFVD
                     EFYMAKIL"
     gene            14617..15036
                     /locus_tag="JD016_08805"
     CDS             14617..15036
                     /locus_tag="JD016_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HAU4999735.1"
                     /translation="MNEREKIIRLWFDMWLKQQDLGMDKIFTEDVVYTESWCPKYENL
                     KTVKHWFNEWNTRGKVIIWDIKQFFHKENQTVVEWYFKNEMNTGSVEEFDGISLIEWT
                     EDNKIKSLKEYGCNLNNYNPYQHSDNPQFRDEKISWF"
     gene            15128..15424
                     /locus_tag="JD016_08810"
     CDS             15128..15424
                     /locus_tag="JD016_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU4999736.1"
                     /translation="MNDIFKDMQVKVGCEYISDLPSYKRKVWHEMKRLNPADYEERQL
                     EDFSKYVFGMSYQTIKDVIKQQKGREEQCRKQGCWWKREEQLAKKQYHTGSTCR"
     gene            15343..15846
                     /locus_tag="JD016_08815"
     CDS             15343..15846
                     /locus_tag="JD016_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAU4999737.1"
                     /translation="MQETRVLVETRGTTGEETISYWFDLPIDVAEFEEKLGVGAESGD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSSDMQEEYTALLTVYENLEALYICR
                     NVITVYHDCKIMIDVARQKLMNDPTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     AIFELPE"
     gene            15864..16367
                     /locus_tag="JD016_08820"
     CDS             15864..16367
                     /locus_tag="JD016_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAU4999738.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            16477..16875
                     /locus_tag="JD016_08825"
     CDS             16477..16875
                     /locus_tag="JD016_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU4999739.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            16853..>17935
                     /locus_tag="JD016_08830"
     CDS             16853..>17935
                     /locus_tag="JD016_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999740.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLKEVAYQKIDEQTEALVSMI
                     GGNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPKKKLNKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDL"
BASE COUNT         6427 a         2687 c         3675 g         5146 t
ORIGIN      
        1 taagcgatag tgtatttaat tgtattaata aatatggatt caatataatt aaatatgcta
       61 ttgatatatt tgtaccctta actattgtta tcttttagat gataatgata tttatttaaa
      121 tactctttat taacaattta taaaacttaa ttgtaaaaaa tacttattaa tagtaaaata
      181 aagtgaatca attatattat aattgctaaa gagagtgagg tgtagctatt tgaaaaaaaa
      241 tagattaata gcaaagaata tatcaatagc atttatagta ctattctttt ttagtgtatt
      301 tacattcttt tatgtaggta atattaatag agtgttagaa tatgaaacaa atgacataat
      361 aacagttaca attgctggat ggattatact ttcattttta tttttaggta ttataattta
      421 catattatat tcaaaagcaa atagtcaaaa aactatagaa aaagtagcat atacggattt
      481 tgtaacaggt tattccaact ggagaaagtt tgaattagat gttacaaatt tattaaagaa
      541 aacatcacaa aataataaat atgcaatggt catattcgat attgataaat ttaaagcaat
      601 taatgatata tatggccata aaaagggaaa tttaatatta aaagatattg cagatacttt
      661 aaatgaattg actgatatca atgaaacttt tgctcgtgta agtgctgata atttcaatat
      721 attattaaca tataacaaaa aagaggatat aataaatatt atcaaaaaga ttatggcaaa
      781 taatgaactt gtaaacttat cttttggaat ttatgaaatc aaagataagg atttatctgt
      841 aagtgtatat agtgatagag cttcacttgc aaaatcatca ataaaaaata atagtgatgt
      901 aaattttgca ttttttaatg acaaattaag agagaaatta ttatttgaag ataagataga
      961 aaaagaaatg gaatatgctc ttgaaagtgg gcaattcgta atgtatttac aaccaaaata
     1021 caatattaaa ttagataaat tttgtggaag tgaagcccta gtcagatggc aatatacaga
     1081 aaaagaggtc atatatccag gtgattttat tcctatattt gaaaaaaatg gatttattag
     1141 aaagattgat atgtacatat tagaacaagc ttgtaaagaa atccggtctt tatttgataa
     1201 aggaatttca cctttaccta tatctgtaaa cttctcaaga gttgattttt ttaaaaaaga
     1261 ttttattgaa aacatagtaa atatatgtga tagatataaa ataccatata gtttaataga
     1321 aattgaaatt actgaatcta gtatgtttgg agatactgat actctattta atgttagtag
     1381 aaacctacag gatattggct ttatagttgc aatggatgat tttggctctg gatattcttc
     1441 tgtaaatatg cttaaaaata taccattaaa tgtaataaaa ttagatagag gattttttgt
     1501 tgatgataaa gatgttgata agagtcaaat tgttataaaa agcatagttt ctctcattaa
     1561 gcaattagga ataagggttg ttgctgaagg aattgaaaca agaagtcaaa tagaaatgtt
     1621 aaaaaaagca aattgtgata ttgttcaagg atattatttt tcaaaaccat taccaattaa
     1681 agaatttgaa aaattagtct ataaaatata gtcagcgagt cttaaatggg aggtggtgat
     1741 taagtgataa gagcaaaatc gttaaaaaat gtactgagta ttgttttaat atttttattt
     1801 acaataattt ctttttctga ttttgcattt aaaactaatg tccaggaaga agaaaatagt
     1861 aaaattaatt taagagaaat atccctacaa agttccaatg ctataaaaat aaaattagat
     1921 agttctctat cctttattaa aagtgtggca aatatggttt cttattttaa tactatagat
     1981 aactcaaaaa tagttccatt attagctaat atatcaaaag aatctgactt tcaaagaatc
     2041 tctattgtat ctcctgatgg aataagtcat accagtgatg gcaaaattat caatgtgtca
     2101 aagtatcagt attttaagaa agctattaaa ggagaaagta atatatctga agttgtgaaa
     2161 tctccaattg atggaaaaga tacttttgtt atagctactc ctgtatttaa taagaataac
     2221 aaaataattg gtgttttata cggttcttac tacaatgata aattagctga atttatagat
     2281 ataacaagtt ttaatagtga agggtacata gatatatttg aatctagtgg tcaatttgtt
     2341 ttaaaatctt cacatacaaa caaactgatt aagtcaagta gtaacatata tgatatattt
     2401 aaaaaagctt cttttgatga tggttatagc tataaaaaaa tatatgaaga tacaaaaaat
     2461 aacaagagtg gctttacctc atatgaatac aaaggagaaa atcgttatgc tacttataca
     2521 cccattggtg taaatgactg gtatgtaatt tctgtgatac cacaatatac tgtaagaaga
     2581 aacattgata aaataaatca attagcattt tctcttattt tcaaggtatt cttattgttt
     2641 attatgctta taacatatat tatttataaa aacaataaat ctcagaaaga tatactaaaa
     2701 gctaacaaag aaatctcatc tattacaaat agcattcctg gaggagtaca aaagtgtacc
     2761 ctgggtgaaa catgcaatat taatttttta agtgatggat ttatagagtt gtttggttat
     2821 acaagagaag aaataaaaac attgtttaat aatgattttt atagtttaat acataaagat
     2881 gatgttgaaa gaataaaaaa agaaattttt aaacaacttt acaaaaataa aactgttgaa
     2941 ttagaatata ggattataac aaaaaatggt aatacagtat gggttttaga taaaggggag
     3001 cttataacag aacctgatgg aaaagaatat ttttgttgtg taactgtaga tattactaga
     3061 acaaaacaat tagaacaaga gatggaacaa aaaaatctag aacttgaaat gatatcagaa
     3121 aatgttttag gtggtataat tataacagaa tttaatgatg attttactat aaaatacgct
     3181 aactctggtt atctttcaat gattggatat acacgtgaac aattagaaaa tgaacttaaa
     3241 tacaaagctt gtaatcttgt tttttatgaa gatagaaaga aggctcttga aagtatacaa
     3301 catcaattaa acacaaaagg aatagctgta tatcaacata gaataagaag aagagatggt
     3361 aatattattt gggtttctat aaaaggaagg cttattgttg atgatgatgg tattgaaaaa
     3421 ggtatttgga ttctaaatga tataacagat ttaaaaaatg cagaacaaaa acttaaaata
     3481 aatgaagaaa gatttagaat tgcattaaag caaacatcaa acactatatt tgagtatgat
     3541 ataaaaacaa agtcactttt aaatactagt aaagagcttg ataaatataa tatttataaa
     3601 attactaaaa atgttccata ttctctagta gaattaggag ttattcataa agattttagt
     3661 gatgatttta taaatatgta tgaaaatgta ataaatggag caaaaaaagc aagttgtgta
     3721 gttaagatga aacttccaga tgaaaattat atatggaata aagttacctt gactacgatt
     3781 tttgatgaag atggtaatcc tgtaagagca attggtatac aggaagatat aagcaaacaa
     3841 aaggaagcag agataagata caatcaagaa ggtaagtatc attcagctat attgtcggaa
     3901 gcaatgataa cttatgaaat aaatgtatca aaggatactt ttatcaaagg aaatgaaagt
     3961 tggcaaaaac catttggaat taagcctgac aataactatt ctcaaatggt aaaaactata
     4021 gcaaatagta ttgtacataa agacaataaa gaaacatttt taaatacatt tttatgtgaa
     4081 aacttattgg atgcatataa aaataatgtg actgaaataa aattagaata tcgaagaatg
     4141 aatcaagaaa ataaaatgat ttgggtcatt tgtacaatgc acttaataaa agattctatt
     4201 acgaacgata taaaagcact tgcttatgtt aaagatattg acaagcaaaa aaatgaagaa
     4261 attagattaa aattaaaagc tgaaagagat actcttacag gattatataa taaaggtact
     4321 acagaaaaat tgattaatga tttcttgaac tctgaggaag caagcttagg aatgcatgca
     4381 tttttaattg ttgatataga taattttaaa tctgtgaatg ataatttagg acatgtattt
     4441 ggagataaag tattatttga agtttctaaa aacctaaaac ctatatttag aaaagatgat
     4501 atagtaggta gaattggtgg agatgaattt atagtattct taaaaaatat tcagtctttt
     4561 gagaatatca aaaagaaagc agaaaaaata tgcgatatat ttagaaattc ttatacagaa
     4621 aataaaaaga catataaaat atctggaaca gttggaattt catttgtacc agaacatgga
     4681 actgtatttg aagaacttta taaaaaagca gacatagcac tttattatgc aaagagcaaa
     4741 ggaaaagata agtatgcact ttataatgat tgtatctacc aaaatgaact tgaaaataat
     4801 ataagtgata ataatataaa gttagcagat aaatgtgaga aatccaaacc agtacttaaa
     4861 gaaattcttg attctattga taattatatc tatatagtga atccaaatac ttatgaactt
     4921 ctttttgtca ataataaggt gtctcgtttg gatttagaga taaaattagg agaaacctgt
     4981 tataaatcac tattaaaatc tgatagacca tgtaataatt gccctcttaa cggattagaa
     5041 gaaaataagc attcaaaatt caaaatagag acaaatacat ttggtaaaaa tatcagtata
     5101 gcaagttgga ttaattgcat agatggagaa tacaactgtc ttatagagtg tatagattct
     5161 tctttataca aaaataaata aaaaaggact atgaagatat gttaaatagt ataactttga
     5221 tacttggtat aatatttaca atttactatt tttatctcaa aatagttttt ggaggaattt
     5281 catttagtga gattttctta gttgtaggaa ttgtactaat tatctatcaa atatttgttg
     5341 taaaaacaca ataaaaatct gattatccgc taaaacacag tatctatcag cattttcaag
     5401 gaaacaaaca catcaattta ctacttattt actactaacg aatgagcctt gatacgcaag
     5461 ttttcctaga tatgcaaaga tatggtacag cacatcagta ttgaaacaag aaaaaaattg
     5521 aataacttat agactatgga acgcctaaca tgacgttcct tttctatttc cagccgttct
     5581 tattggacgg ctattttatt acccaaaatg aaaggagcat tagatttatg aaaaagatgt
     5641 taaaacgatt gtgtacgggc ttcttagctc ttgcaactgt cgttactgct ttaccgacta
     5701 cacccgttca tgcagaaagc aagcaatact ggacggagtc aaaagagcgt gtcggtatcg
     5761 ttgaaaaagt aatgaatgac ggttcgattg gttctacctt taacgaggga catctgaccg
     5821 ttgagagcga ggacgcttac tgtatcgaca ttaacacaga ttttaagaat ggctataaga
     5881 ccagagctga cgcaagcacc cgtatgagtg ccgaccagat aagcgacgtt gctttatcta
     5941 tcgaatatgt aaaacagtac acagacagcc acagcggaat aagcaacaat cacgcctacc
     6001 ttttaagaca gcttgttgtc tggcagagat tgagcgtaca tcttggctgg caatgcgata
     6061 acgtgcgagc ttcttatgat gaaattccaa aggcaacgca ggacgaagtt ttctctggtg
     6121 cgaaagcctt tgtcaaagaa aataaaggac gctatgaatg tggcggttat atctactctg
     6181 gcgaggggca ggaattagga caattctggg cgaaactgaa tgtcggaaat gcgaaacttc
     6241 aaaagacttc cagtaacacc agcattacag acggtaacgg gaattactca atcgctggtg
     6301 caacatatgg tgtctttgct gataaggact gcacaaaaca gcttgccacc cttacgactg
     6361 atgaaaacgg aaatacagat gttgcagagg taaaagcagg cacagtctat atcaaggaat
     6421 tatccgcacc agcaggatat aaagtggata aaaaagtcta tccattgaca atcaaagctg
     6481 gcgaaacagc gactttgaac gtatctgata caccaaaagt aacagacact ttgattgagc
     6541 ttttcaagat tgatatggaa acacagaaag ataatccgca gggaaacgct tctctagcag
     6601 gtgcggaatt tacatggaag tattatgcag gtttctataa caaagacaat ctccctgccg
     6661 aagctactcg tacatgggtt acaaagacaa tcgctgaaac agacagcgac gggacaaccc
     6721 actacatcac aaaattagcg gacgcataca aggtatctgg cgacagcttc tatatgcagg
     6781 acggaaaagc ggttcttcca cttgggacac ttactgttga ggaaacaaaa gctccaaacg
     6841 gctacttgtt agagggtgca tatatgcagg ctggcgataa aaccgaacag attaagggat
     6901 tatatgtaac acagattaca gaggacggcg accttgctgt actatctgga agtaaccagt
     6961 tttccgtatc agacaaggtt atccgtggcg gtgtcaaaat tcagaaacga gatttagaaa
     7021 cgggcgatac gaagccacaa ggaagtgcca ctttgaaaga tactgctttt gacatcattt
     7081 ccttaaatga caattctgtt ttggttgagg gcaagttata caagaaaaat gaagtggtaa
     7141 agacaattcg taccgatatt gagggtatcg cttctacttc tgctgacctc ttaccttatg
     7201 gaaacttccg tatcgttgag agtgaagctc ctaacggata tttgactgac ggtgcaaaac
     7261 cgattgattt tgcaatcaca gaaaatggaa aaatcgtgga cttaaccgac gaagcccgtt
     7321 ctatctacaa tcagattaag cgtggcgata ttgagggcgt aaaaatcggt gcaggcacac
     7381 ataagcgtct tgctgatgtt ccgtttcgga tcacaagcaa gacgacgggc gaaaatcatg
     7441 tagtggtaac tgatgataac ggacaattct ccacttctgc tgactgggct tctcataagc
     7501 acaatacaaa cgcaggcaag accagcgagg acggtgtgtg gtttggaact tctgaaccag
     7561 acgacagcaa gggtgcatta ccttatgata cctacatcat tgaagaatta cgctctgata
     7621 gtaacaaagg ctttgaactt atcccacctt ttgaaatcgt ggtatcaaga aataaccttg
     7681 tgattgattt agggacttta actgatgaat acgaaaaaga aatctctatc cataccacag
     7741 cgaccagcaa agacggcgaa aagactatcc ttgcaggaaa agaggttaca atcgttgata
     7801 cggtcaaatt agacggactt acaaaaggca caaagtatca gctcaaaggc tggcagatgt
     7861 taaaggaaga aaacgccgaa cttcttattg acggaaagcg tgtggaaaat gattatacct
     7921 ttgtcgctga tgaggaagaa atgaaagtgg aaatttccta tacattcaat gcgtccgagc
     7981 ttggcggtaa aaatctggta acatttgagg aattatacga cttgagcaat ccagacgaac
     8041 ccgtgaaagt tgcggaacat aaggacattg aggacgacgg gcaaaccgtt ttaatcacag
     8101 agcgtattat caaaattcat acgactgcta cggataagga cggcaacaaa gaacttgaag
     8161 ctggaaaaga ggttacaatc attgataccg taaccttaga gggcttagaa gtcggcacac
     8221 agtacaaact tgtaggctgg cagatgttga aagaagaaaa tgcagaactt cttatcaatg
     8281 gaaagcgtgt ggaaagtgat tataccttta ccgctgacag cgaaactatg aaagtggaag
     8341 ttgcctttac ctttgacgct acttcccttg acggcaaaca gcttgtaact tttgaagaat
     8401 tatacgattt aagcaatcca gacgagccga agaaagttac cgagcataag gatattgaag
     8461 ataagggaca aacgattacc tttaaggaaa agcccgaagt tccagaagaa ccagagaaac
     8521 ccgaaatacc accgatacca gaaaagccta gcaaaccgag cgacagtccc aaaacaggcg
     8581 acagtacaaa tgtaatggct tttatcgtga tgttgcttac gtctgctggt ggactggctg
     8641 gaacatatct ttacaaacgc cgtaaaatga agaaatcata aggcggtaac ggtatgagga
     8701 aagaaaaatc acgttctccg ccgaagcggt caaacggcag acttccgctt attctggctt
     8761 gtctgcctac aacctgcaag aacacggata gtcaagggtg cgaagcattt attttaccct
     8821 ttactatctg tgggattgct ggtacttccc aaaccgccag aataagaaat cacaatcaaa
     8881 aaacttatca aaatagaaag aaacgaggta aaacaatatg gaaatgaaat atgtcgtgcc
     8941 agatatggca cagtcttttg gaactcttga atttgcagga gaaagcgacc atgtttttga
     9001 cagagataaa gataaccgca gatttttcgc aagaagaagc tacaaccttt attctgacgt
     9061 acagaaaggc gaaaatgttg tggtagaaat tcccgtgcag gctggcgaaa agaagttcaa
     9121 gtatgaacag aaagtaaaac tcgtcaatcc gaagttgtac ggcagaggtt acgcaatcgg
     9181 ggatatggga cataccgatt atgtgttagt tgctgatgat attgtagcag ttgaagaaaa
     9241 gtaaaggagt gaagaattta tgagattatc aaacgggttt gttattgaca aagagaaaac
     9301 atttggagaa ttaaaattta cagcggtgcg tgatgtgttc ttacagaatg aggacgggac
     9361 accgagtacc cagcttaaaa agcgtatcta tgatttgaaa tgcagtctgc atggtggaat
     9421 tatccccgtg tctgtaccgc cagaagtacc gttaagggaa tttccgtaca atgcagttat
     9481 ggagcttgtc aatcccgtag ccgatacggt atcacgaaaa acctatacgg gtgcagatgt
     9541 agactggtat gtaaaggcag aggatattgt attgaagaat aaaggcaatc agaacgcagg
     9601 aagtccacag aactctacac cgcagggaca gccgaaaaaa taggcttgct gaatagattt
     9661 tcattgtagc ggatatagag aactgttata atatgggtat gaagaaacgg ataaatatga
     9721 agttttggag gtaagacatg aaatatttag gcacaatttt agttgcatat atcgttttat
     9781 ttattttaga tatagtgcaa aaaagaaatc ataaaacaac aaaatcttta aatgaatttt
     9841 ctattcgaac aatatcagat gtatcattta tatgtgcaat aggtgctttg tttttgcttg
     9901 gtgttcttat ctggggattt tggagtgaac ctgaaaattt ttattctaaa agatatttga
     9961 tgattattat cccattctct attatttggt ttggagttac tctttttggt atgattgctc
    10021 cgttaaaagg agtgtgggag ataaaagttg aaggagataa tatcacggtt attaaaatgt
    10081 ttatctttag aagatattgg aaaatatcag acatatctta ttgtaaaatg aaacgcggtg
    10141 gtataaatgt gtatgtcaat gggagaaaga aaaaagcatt ttttgttgat ggtatgacag
    10201 accattttga taactttata aaacgaatag aaaaagaggg aaaagagatt attattccag
    10261 agccaaaaga tacgaattga gttggctaga atagtaaatt ccagtttgaa aacttcatat
    10321 ccatatacac aaagcagtta gtcttaggat tgactgcttt tttcataccc agaaaggagt
    10381 gattgattta tgcaaaagat ttttaacaaa ggacaccgta tcagagccag cgacaagcac
    10441 cttgtctacc acttttccat agggacgctt ctgtttgtat tcgtggcggt tcttctgcta
    10501 ctgaatatca aacagctcat gcgtaccgat tgggagcatt tcagcttgtt aaagaacggc
    10561 ttgacgctct ccccttacaa cttcataacc atactgatag cgactggtgt ttgtgcattg
    10621 gtcgcttttc tgtattaccg cttctgttac gacagtttca agaagctcct gcaccgtcaa
    10681 aagctggcaa aaatgatact ggaaaataag tggtatgaag ccgataccgt acaagacagc
    10741 ggttttttta ctgacctgca aagcagatca agggaaaaaa tcgtctggtt tccgaagatt
    10801 tattatcaaa tggaaaaggg actgcttcat atccgctgtg aaattacgct gggaaaatat
    10861 caagaccagc ttttacggtt agaggataaa ttggaaagtg gcttgtattg tgaactgacc
    10921 gacaagaccc tgcatgacgg ttatatcgaa tataccctgc tttatgatat gatagcgaac
    10981 cgcattacca ttgatgaagt acgggcagaa aacggcagtc ttagactgat gaaaaatctt
    11041 gtctgggaat atgacgcact ccctcacgct ctgattgctg gtggaacggg tggcggtaaa
    11101 acttatttcc tgctgacgct cattgaagcc ctactgcata ccaacgctgt cctttatatc
    11161 ttagacccga agaacgctga ccttgcggac ttagggacag ttatgggaaa tgtgtatcac
    11221 accaaagaag aaatgataga ttgcgtcaat gccttttatg agggcatggt acagcgaagt
    11281 gaggaaatga agcgacaccc gaactataag acgggcgaaa actacgccta tctgggactt
    11341 ccaccctgct ttcttatctt tgatgaatat gtggcgtttt ttgaaatgct ggggacaaaa
    11401 gaaagcgtag gcttgcttag ccagttaaag aaaatcgtta tgttaggacg acaagcagga
    11461 tatttcctta tcgttgcctg ccagcgtcca gacgcaaagt atttttcgga cggtatcaga
    11521 gataacttca atttccgtgt gggattgggg cgtatcagcg aattaggtta cggtatgctg
    11581 ttcggttcag atgtgaaaaa gcagtttttt caaaagcgta tcaaggggcg tggctattgt
    11641 gatgtgggaa caagcgtgat aagtgaattt tacacgcctt tagtccccaa aggacatgat
    11701 tttttacaaa caatcggcag gcttgcacaa gaaaaacaag tcatgccaga acaacaagaa
    11761 tgaaaggaaa aaagaatgga atatttacga acaacaactt ataacagaat aactggaatg
    11821 aatatgtctg gtgtatgtat caattatgat aatttttgga atgatgtgag aatgactgct
    11881 gactgtgaat ttgacaagga cgagtattct ccaacagaac gttatcataa tcgccttgtc
    11941 gaaattatga aaaatgtatg gagtggcaaa aaaagacttc cagttgtatt ttctattcgt
    12001 ttagaaaaat atatttcatt ggttgattat ccagtatgct atacatttgc tgttattgat
    12061 aagggatttt tcaagcgtac ttatcgtaaa gaggaaattc ccgaagaaat tttaaaggag
    12121 tgtattgcaa aggataatga ctgtatcgtg ttttatgtag gtatgaaccg ctaacagaca
    12181 caacggcagg aacggtcagc gaagcgaccc agaccgtgta tgccgttgct ggtgtggcgt
    12241 aagccacgcc agcaggtaaa acccctcggt atctaacaga ggggtacgtt tgcaaataga
    12301 caatagacaa aaaacatagg aaacataagg cttctggcat ggtttcctaa caaaaatcga
    12361 ctgtgaagtc gccttaaaaa acttcacact tgacagattg ggggtatggt tctgaatgaa
    12421 gaacaatgga taaaagaatt acgggagaaa cggattgctt acggtatctc acaaggcagg
    12481 ctggcggtgg cttctggtat cacaagagaa tatctcaaca agatagaaag cggaaaaatg
    12541 aagccgtcaa aggaacttct ggaaactttg cataaggaat tggcaaggtt caatccagaa
    12601 gcaccgctta ccatgctgtt tgattatgta aaaattcgtt ttcccacgct ggatatacag
    12661 cacataatca aagatatatt aaaactgaat gtcaattata tgctccatga agattacgga
    12721 cattacagtt atacggagca ttattcttta ggggacatct ttatctatac gtcggctgac
    12781 gaagaaaaag gtgtcctttt agaattaaag gggcgtggtt gcaggcagtt tgaaagttac
    12841 ctgctggcac agcaaagaag ctggtatgac tttctcatgg acgcattagt agacggtggc
    12901 gtgatgaagc gtatcgacct tgctatcaac gaccatacgg gcattttgga tattccagag
    12961 cttgcggaga aatgcaggaa acgggaatat atcggaaagt ccagaagcta taagttttac
    13021 cagtcgggcg agcttatcaa gcacagagag gacgacagag aatatatggg acgtactctt
    13081 tatcttggtt cgctgaaatc agatgtgtat ttctgtatct atgaaaagga ctatgagcag
    13141 tacgtcaagc tggggacacc gctggaagaa gccgacatta tcaatcgttt tgagatacgg
    13201 cttagaaatg aacgtgccta ttatgcagta cgagatttgc tgacctatta tgacgcagag
    13261 cagaccgcct tttcgattat caaccagtat gtgcggtttg ttgatgaaga accagacaag
    13321 cggaaaaatg actggaaact taatgaccgc tgggcttggt ttatcggcga taacagacag
    13381 agcttaaagc taacgacaaa gccagagcct tacaccttag accgcacatt gcggtgggta
    13441 caaaggcagg tagcaccgac cttgaaaatg ctgaaaaaga ttgataaagg aaatggtaca
    13501 gactacatgg aaacaatcga acagcaggca aagctcacag aaaagcatga aatgataatc
    13561 aaacagcaga cgacccctac aaaagatttg gtggaaagtt aggagtgatg aaaaatgtgg
    13621 gtattattaa aagacttcct gctggtatct atgggaatgg gtatcggcgt agtcttgatg
    13681 tgtattttga atgtcggcaa agaagctgac tatgaaatga aacaattaga agaaagcgag
    13741 gacaattaaa tgaatttcgg acagaattta tatcaatggt ttttatcaaa cgcacagagc
    13801 ttagtgctta tggcgattgt ggttattggt atctacttag ggttcaagcg tgagttttca
    13861 aaacttatcg gcttcttggt agttgccttg attgctgtcg gcttggtgtt caacgctggc
    13921 ggtgtgaaag atgtattgtt agaactattc aataagatta tcggtgcata aaattttatt
    13981 gtggtgctga tatgagaatg gtataattta gaatatgaaa taaagtgaat actatttgag
    14041 gttgaaaata tgattattta tagcaaattg gaaatcaata gaataaaaga tttttgggag
    14101 ttattaaatc gtttagatac tgaaacagat tacatgatgt acgaaccaaa tgaaagaaaa
    14161 gcgaaaacta ctattcaaga attaaaatta gatattcaag acaatgtaat tcaaggtttt
    14221 gattttttgc aagtggcaac agaaaatgac aaaatagttg gttatattag agcagaaaga
    14281 ggaaaatttg aaagaaattt tcatacggca tatattgtta taggtatctt aaaaaaatat
    14341 agaggaaaag gtattggaac tacttttttt aataatttag atttatgggc taaaacaaat
    14401 aatattgtcc gtttggaact gacagttgaa tgttgtaata atactgcaaa aaatttgtat
    14461 gaaaaaaatg gttttaaaat tgaaggaata agaaaaaaat caatgtttgt tgctggttcc
    14521 tttgttgatg agttttatat ggcaaaaatt ctataaaata aattttcaac ttcattattt
    14581 acaaagtaat tggtcttagg attgaggtgt tttattatga atgagagaga aaaaatcatt
    14641 cgcttatggt tcgatatgtg gcttaaacag caagatttag gaatggataa aatttttaca
    14701 gaagatgttg tttatacgga aagctggtgt cctaaatatg aaaatcttaa aacagtaaaa
    14761 cattggttta atgaatggaa tactcgtggt aaagtcatta tttgggatat taaacagttc
    14821 tttcataaag aaaatcaaac tgtcgtagaa tggtatttta aaaatgaaat gaatacggga
    14881 agtgttgaag aatttgacgg tatttcctta attgaatgga cagaagataa caaaatcaaa
    14941 tcattaaaag aatatggttg caatttgaat aactacaatc catatcagca cagcgataac
    15001 cctcaattta gagatgaaaa aataagctgg ttttaaaact taatcaaatt atatctatac
    15061 acacaaagca gttagtctta ggattgactg cttttttctt acctaaattt cagattggag
    15121 tgatgaaatg aacgatattt ttaaggatat gcaggtaaaa gtcggctgtg aatacatttc
    15181 cgacctgccc tcttacaagc gtaaggtgtg gcatgaaatg aaacgactga accctgccga
    15241 ctatgaagaa agacagttag aagatttttc aaagtatgtg tttggtatgt cgtaccagac
    15301 cataaaagat gtgataaaac aacagaaagg acgtgaggaa caatgcagga aacaagggtg
    15361 ctggtggaaa cgagaggaac aactggcgaa gaaacaatat catactggtt cgacctgccg
    15421 atagatgttg ccgagtttga agaaaagctg ggtgtcggtg cagaaagtgg ggattaccgc
    15481 attatcgaaa aggtgttgcc ttatgctgat gaagtccacg aacataccag cgtgtaccag
    15541 ctcaacgaat tagattttat gtaccgccag ctttcgagcg atatgcagga agaatataca
    15601 gcacttctta cggtgtatga aaatttagaa gcactttata tttgcagaaa tgtgattacg
    15661 gtttatcacg actgcaaaat catgatagat gttgcaaggc aaaagctgat gaacgacccg
    15721 acgttcaaac atctgtccga ggactgtcaa gaatattatt ttgactttga agcctatgct
    15781 tctcacttgc aggaacacgg gaaattttta gtaacggaac acgctatctt tgaactgcca
    15841 gagtaggaaa tgaggtggtg cttatgattg atgatatggc ggtttacatt gctaatcttg
    15901 gcaaatacaa tgagggctat ttagtcggtg cttggttcac gtttcctatt gacgaggaag
    15961 atgtgaaaga aaaaatcggc ttaaatgaac aatatgagga atatgctatc catgataccg
    16021 acaacttccc tattgcgatt ggagagtatg tttccattga agaactcaat gaaatgtatg
    16081 aaatgataga ggaacttccc gactatatcg tagagtgtct ggacgaattt atcagccact
    16141 acgggacgct ggaagaagtc gtggaacaca aggacgatat ttattattat cccgactgtg
    16201 aaaccatgac agacgttgcc tattactaca tagacgaatt gcaggcacta ggggatattc
    16261 cacccagctt acagaactac attgactatg aagcctatgg gcgagatttg gatatgggtg
    16321 gttgctttat tgaaacaagc cgaggtatgt gcgagatacc atactaacgc tggaagatca
    16381 gcgacaagca ttgttgctac tgacagcgta tttctctttt aagggacagt ctgaaacatg
    16441 gctgtccttt tctattttga aaggagctga aagaacttga aaaagattaa atcttatacg
    16501 ggtatctgga acgtggaaaa agtcttgtat gcaatcaatg actttaactt gccctttccc
    16561 gttaccttta cacagattac atggtttgtg attacagaat ttatcatcat tctgtttggg
    16621 gatattcccc cactttccat gattgaggga gcatttctca aatactttgg tattcctgtt
    16681 gctctcactt ggtttatgtc gcagaaaacc tttgacggaa agaaaccgta cagctttttg
    16741 aaatcacaga taacttatgc gttgcgacct aaaatcactt atgcaggaaa agccgtaaaa
    16801 ctgcataagc agatattgaa tgaaacaatc acggcagtaa ggagtgtgaa ctatgttccc
    16861 gataaaatat attgacaata accttgtctg gaacaaggac aatgaggtgt tcgcctatta
    16921 tgagctgata ccgtacaact attctttctt atccgcagag cagaaattta tcgtgcatga
    16981 cagtttccga cagctaatcg cacagtcccg tgagggaaaa attcatgcct tgcagattgc
    17041 cacggaaagc tccatacgaa gtatgcagga gcagtcaaag aagctggtaa cgggaaaatt
    17101 aaaggaagtt gcctatcaga aaatagacga acagaccgaa gcgttggtat ctatgattgg
    17161 gggcaatcaa gtggactacc gcttttttct tggttttaag ctcatggtta cggaagaaca
    17221 gctcaatctg aagaacatca aaaaatcggc gtggctgacg ttcacggaat ttctccatga
    17281 agtgaaccat acgctgatga atgattttgt ttccatgccg aatgatgaaa tcaaccgtta
    17341 catgaaaatg gaaaagttac tggaaaataa aatctcccgt cgctttaagg tgcgtcgctt
    17401 ggaaatcaac gattttgggt atctcatgga acatctttac ggcagggacg gtatcgctta
    17461 tgaagattat gagtaccagc taccaaagaa aaaattgaac aaagaaacgc tgataaaata
    17521 ctacgacctt atccgtccga caaggtgtgt gattgaggaa agccagaggt atttaagatt
    17581 ggaacatgag gacaaggaaa gctatgtatc ctattttacc gtcaatgcga ttgtcgggga
    17641 gcttgatttt ccgtcgtctg aaatcttcta tttccagcaa cagcagttta cattccccgt
    17701 tgatacaagc atgaatgtag aaattgtgga gaacagaaag gctcttacca cagtaagaaa
    17761 taagaaaaag gaattgaaag accttgacaa tcacgcttat caagcaggaa gtgaaaccag
    17821 ctcaaatgtg gtggacgcat tagacagcgt ggacgagctg gaaacagact tagaccagag
    17881 caaagaaagt atgtataagt taagctacgt gatacgggtg tctgcacccg atctt
//
LOCUS       DACXMZ010000067        17801 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.067, whole genome shotgun sequence.
ACCESSION   DACXMZ010000067 DACXMZ010000000
VERSION     DACXMZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.067"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            78..413
                     /locus_tag="JD016_08835"
     CDS             78..413
                     /locus_tag="JD016_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999741.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            550..1827
                     /locus_tag="JD016_08840"
     CDS             550..1827
                     /locus_tag="JD016_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HAU4999742.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKEQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1948..3336
                     /locus_tag="JD016_08845"
     CDS             1948..3336
                     /locus_tag="JD016_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAU4999743.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3716..4420
                     /locus_tag="JD016_08850"
     CDS             3716..4420
                     /locus_tag="JD016_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HAU4999744.1"
                     /translation="MSKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDPVVT
                     FVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISIEVG
                     LSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDAPVS
                     NSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNVARG
                     IIEEYIKECNIINLNK"
     gene            4556..5407
                     /locus_tag="JD016_08855"
     CDS             4556..5407
                     /locus_tag="JD016_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAU4999745.1"
                     /translation="MKLTKLNIKKYRAPIYKYALANINLRSAKSTNSSIITVIPQGAK
                     MEVLDEEDDWIKVMYNSQEGYVYKELVSVSEYAWSNLNLREDKSTTSNIITVIPEKSR
                     VEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNENNIKST
                     SDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRKPSFGTD
                     EYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWIYDNIPD
                     TTTVIIH"
     gene            complement(5562..6707)
                     /locus_tag="JD016_08860"
     CDS             complement(5562..6707)
                     /locus_tag="JD016_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAU4999746.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(7040..7816)
                     /locus_tag="JD016_08865"
     CDS             complement(7040..7816)
                     /locus_tag="JD016_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAU4999747.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADAINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8398..9372
                     /locus_tag="JD016_08870"
     CDS             8398..9372
                     /locus_tag="JD016_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HAU4999748.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9470..9775
                     /locus_tag="JD016_08875"
     CDS             9470..9775
                     /locus_tag="JD016_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HAU4999749.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            9930..11090
                     /locus_tag="JD016_08880"
     CDS             9930..11090
                     /locus_tag="JD016_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HAU4999750.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            11140..12042
                     /locus_tag="JD016_08885"
     CDS             11140..12042
                     /locus_tag="JD016_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAU4999751.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            12122..13015
                     /locus_tag="JD016_08890"
     CDS             12122..13015
                     /locus_tag="JD016_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HAU4999752.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      13068..13249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13468..14607
                     /locus_tag="JD016_08895"
     CDS             13468..14607
                     /locus_tag="JD016_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAU4999753.1"
                     /translation="MKEMKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGK
                     KLELKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLI
                     ASTTQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVD
                     LNPDLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKE
                     DMATSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGI
                     NACASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKN
                     GNLWVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            14679..15704
                     /locus_tag="JD016_08900"
     CDS             14679..15704
                     /locus_tag="JD016_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAU4999754.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     TIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            15707..16903
                     /locus_tag="JD016_08905"
     CDS             15707..16903
                     /locus_tag="JD016_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4999755.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(17031..17756)
                     /locus_tag="JD016_08910"
     CDS             complement(17031..17756)
                     /locus_tag="JD016_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAU4999756.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSVDGKESFKSARAYADENFIYRVGETIIAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT         6834 a         1976 c         3102 g         5889 t
ORIGIN      
        1 gtatatttat aatattaata aatatattgc tataaaagtt tgtttaataa attaaataaa
       61 gaaaagagga gaataaaatg tacaacatag atgatatgat atataattgt ccagtagaag
      121 ctttatcaaa tattttaggt aaaaaatggg tggctataat tatatggaaa atacaagaag
      181 ataaaaaacg atttggagag ctacaaagag ctataccaga ctgtagtaag aaaatgttag
      241 ttcaacaact agatttttta attgaacagg ggattatagt caaccaaaaa aagttggtta
      301 ataatgttgt agagtcaaca tattttttaa gtgaatcagg attacgattg cttcctgtaa
      361 tggaaaaaat gattatgtgg agcaataaaa atttaaattg tgataaatac taaaatttac
      421 tctgtaagta atgaaaattt aattaagcta tgagtagaaa aatgtattaa aattacataa
      481 ttttttagtt agattttaat ttagaaatgg gattgtaata ttggaaataa aataaggagg
      541 aattttaatt tgcaaaatgt aaatttattt ttagtattta tagaaggtat agtatcattc
      601 ttttcaccat gtatactgcc aattttgcct atttatttga gtatattgtc aaatagtagt
      661 gtagaaaatt taaaagaagg aaagacgagt tttataggaa gttctttatt taaaaatact
      721 atattttttg ctcttggaat ctcaacaaca ttctttatat tgggttcatc agtaaaagta
      781 ttgagtatgt tttttaatga aaataaagat ttaataatgt ttattggtgg gattataatt
      841 attatcatgg gattatttta catgggaata attaaatctt caatattgaa tagagaaaaa
      901 agatttaatg ttaagtttaa agaaatgaaa gcaataacag cttttatatt aggttttacg
      961 tttagttttg gatggacacc ttgtattggt ccaatattgg catctgtatt agtcatggtt
     1021 tcaagttcaa gtaatcatct tagtgccaat cttttaatag cagtatatac tattggtttt
     1081 attttgccat tcataattac agctatgttt tacagtaaac tatttaaaac tatagataag
     1141 ataaaatcta acatggaaat tattaaaaaa ataggtggga ttatacttat agtatcagga
     1201 atattaatga tggtaaatgg atttggaagt ataagtaaac actttaatac gtcacaaaat
     1261 agtaaaattg aaagcaagca agaagaaaat aagcgagaaa attctactga taaagaagaa
     1321 aattctgatg gtaatgatag tcaaaaagac tctaataatg acaataatga taaagggtct
     1381 aatgatgaag atagaataaa gtctattgat tttactttaa cagaccagta tggtaaaaca
     1441 cataaattaa gtgattatga aggcaaagtt gtattcttaa atttctgggc tacatggtgt
     1501 cctccatgta aagaagaaat gccttatata gaacaactgt ataaagacta taataaaaat
     1561 aatgatgatg tagtaatttt aggagtagct tcacctaatt taggtagaga aggttcaagg
     1621 gaacatgtag ttaacttctt aaaagaacaa ggttatacat tccctgttgt acttgatgaa
     1681 gatggagcat tagcatatca atatggaatt aatgcttttc caactacatt tataattgac
     1741 aaggaaggat atgtaactca gtatattcca ggagctatgg ataaggcaac tatggcatca
     1801 tttattgaaa atcagagaaa taaataaaag tatttaagta taagtttata aatcaattta
     1861 aggttacaaa aaagtgccta attgtaccac ctaaataatc ttgttatagt aaaagtataa
     1921 acaatacaag aaagggttga attttatatg aaaaaaacat ttgatgctat aataatagga
     1981 tttggaaaag gtggaaaaac attagcaggg gatttggcaa acaggggatt aaaagtagct
     2041 ttaatagaaa agtcaaacaa gatgtatggt ggaacatgtg taaatgtggc atgtatacca
     2101 acaaaaagct tagaaaattc agcaaacagc gtaaaaacaa agaatataaa ttcttgggat
     2161 gaagtacaag ctgaatatga gaaagctata gataaaaaag aaactttaat tacaaaactc
     2221 agagaagcta actataataa attaaattca aatgagaatg taactatatt cacaggaatg
     2281 ggaactttca ttgatgaaaa aacagttcaa gtcaaaactg aaaatgaaat ttatgagtta
     2341 gtagcagata atatatttat taatactggt tcaagaccat ttatacctaa tattaaggga
     2401 attgaaaata agaatatagt atatgatagt gagtctctta tgaatctaag agttttacct
     2461 aaaaaaatga caattatagg agctggattt ataggtttag aatttgctgg tatttatagt
     2521 tcatttggag cagaagttac aatattaaac tctaataatg gcattttacc aaatgaagat
     2581 gttgaggatt cagaagaaat aataaaatta cttgcaaaga gaaatgtaaa aattgttaat
     2641 aatgcaaata taaaagagat taaagaagtt tctgaattag caatagtaga atatgaagta
     2701 gatggtaaat ctaaagaatt aacaagtaat atgatacttg tagctacagg aagaaaagca
     2761 aacactgaag ggctagggct agaaaatgca ggtatagaat taaatgaaag aggatttatt
     2821 aaagtctcag aaactcttaa gactaataaa gagcatatat gggctatagg agatataaat
     2881 ggaggtccac aatttactta tatttcattg gatgattatc gcatagttat taatcagtta
     2941 tttggagata agactagaac aactaatgat agaaagaata tcccaaattc tatatttata
     3001 agtccagcat tttcaagagt tggtcttaat gttaagcaag ctaaagaaaa gggatatgaa
     3061 gtattagtgg caaagatgcc agtagaagca attccaagag ctaaacagat tggcaaggca
     3121 gatgggttta taaaaatagt gatagataaa aaatccaata agatacttgg agctagtatg
     3181 atatgtgaaa attcaagtga gattatccat cttattcaat tagctgtaga tttagaagta
     3241 gagtatacat atttaagaga ccgtgtttat gcacatccaa ctatgacaga agctcttaat
     3301 gatattctat ctcctaatat gataaaagaa gtataacata agtttaatat ataaacctaa
     3361 aattatcatt ataagtagat tttagaaaga gactatctca aaaaatagag ataagtctct
     3421 tttttaatgt ttaaataata aaaatatatt tgattttata aaagaagatt attttatgag
     3481 ctaaaaagtt tatttagaaa cactacctat ttatgttata aagttataaa tataatatag
     3541 aatctattat tctgtattat taagtggggt taagcgttat aattaattat aaatattgaa
     3601 taatctaaat ggataagata atggtattaa aaattatctt aaaaggtgaa ataatctgta
     3661 tttaatgtag tacagttata tctttaaagc aaggtggtga aatgatataa ataagatatc
     3721 taaaagaggg tttgatgata gtgataaaag atggttttca agtaaagaat taacaagact
     3781 tataaaggct aaagaagaaa tagaatggtt attaaatagg gattacaaaa tagacccagt
     3841 cgttactttt gttggtgata gatatcagtt ttctataaga caaagagatg cattaaaaag
     3901 agcagtctgt actgatgaaa aaaatattat aagacaatca aagaggcttt ctctagataa
     3961 aatcaaagaa gataccgtaa atatagatgg ctttaatttg ataatatcca tagaggtagg
     4021 attatctggt ggtactctag taataggaag tgatggcaat ataagagatt tagcaggtct
     4081 taggggaacc tataagatta tagataaaac agaagaagct ttgaatctga taggtaaatt
     4141 ttttaataaa tacaatgcac agaatcttaa attttattta gatgctccag tttctaattc
     4201 aggaaattta aagtatagga tattagagca tgccaaaact tggggaattg aaactgaagt
     4261 agaattagta aaaaatgcag atgttgtatt ggaaaaatta gatagagtag ttagtagtga
     4321 tgcagtcata gttgacaagt gtataagtta ttttaatgtg gctagaggta taattgaaga
     4381 gtatattaaa gaatgtaata tcataaattt aaataaatag ataaatatag gtgagaatat
     4441 ataagtatat gtagcaatat tcttagtaat acaagtaaag tttgaaaata ggagaacgaa
     4501 tacatatcga atttcataaa atgttgtaaa aagacatatg agaggatgga tatgtgtgaa
     4561 attaactaaa ctaaatatta aaaaatatag agcacctata tataaatatg ctttagcaaa
     4621 tataaatttg aggtctgcta aatctaccaa ttcaagtatc ataacagtaa tacctcaggg
     4681 agccaaaatg gaagtattgg atgaggaaga tgactggatt aaggtgatgt acaattctca
     4741 agaaggatat gtgtataagg agttggtatc agtaagcgaa tatgcttgga gtaacttaaa
     4801 cttaagagaa gataaatcta caacttccaa tataattaca gtaattcctg aaaaatctag
     4861 ggttgaagtc cttcaagtag atggagattg gagtaaggtt gtatatgatg ataaaatagg
     4921 atatgttttt aattattttt tatcaattga tgggaataaa ccaaatgaat tagattataa
     4981 gtatttttat actgacatga ttaaatttgt taacgaaaat aatattaaaa gtactagcga
     5041 ttatctaatt gtaacagacc ttagaaataa atatacttat atttttaaga aagataatgg
     5101 aggatgggga caactttaca agtggcaatg tactataggc aagcctgaga ctccaactat
     5161 aacaggtata ttttatataa gtggaagaaa accttctttt ggaacggatg agtattcagt
     5221 taagtatgct actagaatta aaggtggata ttactatcat tctgtactat atgattcaac
     5281 aggaagttat ataatagatg gcagacttgg tgaagctctt agtcatggat gtataagatt
     5341 aagtacggaa aatgcaaagt ggatatatga taatatacca gatacaacaa cagttataat
     5401 tcattaagaa aaattttgag ttttttattg ggataatgat tcttaaatta ataggattat
     5461 atattaactt ctcataaaga gtactattta aaaagtgtaa taagagagtg ctattaacaa
     5521 aacaaagata ttttgaggca ctctcttata aatatacata attaagatat tttcataaac
     5581 aattctataa tcttatcttt atcaaagaaa acaggattag caccagcaca agcatctttc
     5641 atagagtttt ctgcaagcaa ttcaaaatta ggattatcaa cacctacatc ttttaaagat
     5701 tttgggattc caacctcttc agaaagagct tttatcttat ctattacaaa gtcaacacac
     5761 tctttatctg atttattatt tacatccatt ccaataactt cagctattgg tcggaatttg
     5821 gcaggggctg atttagcatt ttcctcctca actattggaa gtagcatggc attacatact
     5881 ccatgaggta aatcatataa acctcctaat tgatgagcca tagcatgtac atttccaaga
     5941 cctgcattac taaaggcaat tccatttaaa aaacaagcat aacacatttg ttctctagct
     6001 tctatgtcat taccattttt aacagctctt ggaaggtatt taaatatctg ctttatagca
     6061 tataaggcag ttgaatctgt aacatcataa gcaccattag ctactacagc ttctatagca
     6121 tgagttagtg catccatacc agttgctgct gttaaattag atggtttgct aatcattaat
     6181 tctgggtcat ttactgtcat tgtggctagt gaattggtat caaccattat catctttaca
     6241 tgtctgtctt catcagttat aacatagttt attgtaactt cagcagatgt accagcagta
     6301 gtagttatag ctatgatagg aagacatttt ttagaagttt tgttgatacc ttcatagtct
     6361 ctaatatctc ctccattagt ggctaatata gagatagctt taccacagtc ttgtggagaa
     6421 ccacctccta tagttataac gaagtcgcaa ttttctgatt ttaatatttt aagtccttta
     6481 ttcacattag ttacagttgg attttgttta acttcatcat aaacaacaaa gtctatatct
     6541 atctgagata aaatatctgt tacttttttt acagtaccat ttgaaactag aaatttatca
     6601 ctaacaataa atgctttttt agttcctaat gatttaattg aatctttagc ttcattaaga
     6661 catcctttcc caagtaaatt tattggtggt acataataaa tgctcataac taaaatctcc
     6721 ttttattttc attgattttt atgtaaaaat atatatctaa caattttcac taatttactc
     6781 ataatatatc gtatcttaga ttgagatagg gttgctggtt cttatataag aacttaattc
     6841 gtaattacga ttatgattag aatttaacat agtattttta tcatgtcaat aaaaatataa
     6901 aaaatattat aaaaaaaaca aagttactgt agttaaatca gcaactttgt ttttatagta
     6961 taaatcaaat aaatacatta gtttaaaaca aaatttttaa ttattatgtt ttgatatctt
     7021 ccatatacag aatattaatc taatttatta caatgtcttc attacgttct tccatcattg
     7081 attgaatatt tttctgagca tttaagagta atttttttat cagttctatt ttttcgtcat
     7141 tagctctaaa taaaggcagt gttacactaa cagcagctct tatacatcca ttttttctta
     7201 ttggtacggc aagacatttt aggtgttcca ttgattcctc acactctgaa gctatttgtg
     7261 ttctttgaac ttcaagaagc tggtcgtata aaacatcaaa atctgtaatt gatttttctg
     7321 taaaaccttt tagaccatct ggatataagc ttattagttt ttccttgtca aaatcacata
     7381 aaagagcttt tccaagacct gtacaattag caggtatttt ttttccaaca taagaaataa
     7441 gtctaatcgg ttctggagaa tctacttttc caatataaag aacttcatta ttgtcaagta
     7501 taccaagctg acaagtttca gaacaggaat taactatatt agtcatttcg tcaattatat
     7561 agttaaaaat agttcttttt tcaaaaaatg aagaccctaa tgtgtaagaa cgtatcccta
     7621 tagaatattt ggatgtgttt ttatcaatag taatgtattt tctagcatta agtgtatgaa
     7681 ctataggaaa gatgctactt tttggagcat tgatagcatc tgctatttct gtaagagtat
     7741 atccattttc ggattctgct aatgcttcta aaatatctaa tatcctagct gttgggcgat
     7801 gattatctga agtcataaat ttccccctct ttttattaat ttaaagtatt agaatatgtt
     7861 gattttagca cactatacga tattttacaa tgtagaaaat aaatatgtaa atttatatat
     7921 aaataagctc ttgatttaga tacagataga tattttttta gactgtacat taaggaataa
     7981 ctaatttcca aaatatacct aaagaaagta agtgatattt agttatataa tttaagtaaa
     8041 tatatgcaat agttattttt atatgtaaat atcaaaatat tatgaactta tattttatct
     8101 attttttaca ctgtataaaa actttatagt tttgaatact atataatttt aaatacgctg
     8161 tatttttgca tataaggaag tcatttgata atatacaagg tattatcaga aagtttgaaa
     8221 aaattttgac aaaaatttat tttttttatt gactcataaa taggaaaatg ctatcattaa
     8281 ttcaaagatt cgtaattaaa aacttagttc ttaaatacga accgaaacgg ccgttagaat
     8341 taaagctaaa aacttaataa gagtaattaa atatcatgtt ttaggaggat taactttatg
     8401 ataatggatt ttattaaaaa aattcctgca ggtatgatga tagtgccaat gtttatagca
     8461 gctttcatta ataccttttg cccagatatt gtacaaatag gttcgtttac gacagcagta
     8521 ttttcaagtg ttgggtctgc aactataatt ggggttcaac tggtatgctt gggaactcag
     8581 cttcaattta gagaaatgcc aaaagtttta aagagaggtg gtatacttct tggttctaaa
     8641 tttgctatag gtgctttgat aggtatactt ataggaaagc tatttggaat ggatggtgtc
     8701 cttggtctta caacattggc tgttataagt gcagttacaa atagtaatgg tagtatatat
     8761 ctttcattga tgacttcata tggagatgaa acagattgtg caactatgag tttacttact
     8821 ttaaatgatg gaccattttt tacattaata gctcttggga cttcaggtct tgcaaatgta
     8881 ccattgttgt cattattggc tgcaatagtg ccaatacttg ttggtatgat aataggaaat
     8941 tttgacaaaa aaatgaaaga gtttttagag ccagggtgta atttactcgt accttttgta
     9001 gggtttgcac ttggtagtag cataaatctt gcaagtatac taaaaggagg tttatcagga
     9061 gtattacttg gattaatatc tgtatttgta ggaggtatat ttatagtatt ctgtgataaa
     9121 tttataggaa aacgtccagg atatgcagga tgggctgtag ctacaactgc tggaaatgca
     9181 attgctgtac ctgctgctgt tgcattagtt gacccatcat ggcaaccata tgttgcaaca
     9241 gctacaacac aagtggcagc cgcagtagtt gtcactgcaa tattagtacc tcttataact
     9301 agttggtggg ctaagaagta tggatgtcct aagttcccaa aacaaaatga ggatggtaca
     9361 ataacagctt aatttatatt acagtgagtt gttggtaaaa taataaattc tttaattaga
     9421 ttttaggata ttaataaaat tatattattt ataaaggaga gcaagaataa tgataaatgc
     9481 agtgataatt gatgaaaaag ataatgttgc agtagctatt gaagcaatag ctaaaggtag
     9541 tgaaattagt tttaaattaa aagacaaaac agtaaaaacg attacagcac tagatgatat
     9601 aactatttat cataaattgg ctataaaaga tatggcccaa ggtgataaag ttactaaata
     9661 tggtgaacat ataggagaag caaatcagga aataaaaagt ggtcaacatg tacatattca
     9721 taatgttaga agtgttagag aggatttgga taaacaagtt atgactaata tataaaataa
     9781 ttttatagag ttataaaagt tgtattcatt gcttttagtg tcaaatagtt tttaaatagg
     9841 tttttagctg tttgattagt gagtgtaata aaaattataa tatataaaaa ttataaaaag
     9901 tatatagact attttaggag gaaatcagta tgaaatttta tggatataaa agaccagatg
     9961 gaagagttgg tataagaaac cacatattaa tattgcctac gagtgtgtgt gcatcagata
    10021 caactagaat aatagcatca caagttcaag gggcagttac ctttaataat cagaatggat
    10081 gttcacaagt acattcagac caacaattaa ctatggatgt tatggctggt tatgcagcaa
    10141 atccaaatgt atatggtgta atagttgtat ctttgggatg cgaaaattgc caaaatgatt
    10201 tggtagttga tgcaataaaa gagagaacaa ataagccaat aaaaactctt gtaatacaag
    10261 aagaacatgg aactttaaaa actatagaaa aagctgttcg ttatgctaga gaaatggcac
    10321 aagaagcttc tttgcttaga aaagaagaat ttccaatatc tgaacttata ctaggaactg
    10381 aatgtggtgg gtctgaccca acaagtggat tagcagcaaa tccattaata ggtgaattaa
    10441 gtgataaact tgtcgattta ggagcaacat caattttaag tgaaactact gaatttatag
    10501 gtgcagagca tatactagct aggagagcag taaatgaaga agtaaaagaa aaaatattac
    10561 atatagtaca tagatatgaa aattcattaa aattggttgg agaagaagtt agagaaggta
    10621 atccatctcc tggaaatata gctggtggtt taacaagttt agaagaaaaa tcacttggtt
    10681 gtatacataa aggtggtcat agacaaataa gtgaagtata tgattatgcc aaacaaattg
    10741 ataagaaagg tcttgttata atggataccc ctggaaatga tgcttcatct gttgctggta
    10801 tggtagcagg aggagcacaa atagtggtat ttagtacagg aagaggaaca ccatcaggaa
    10861 atccaatagc accagtaata aaaataactg gaaataaaat aacttttgca aatatggaag
    10921 ataatatgga ttttgatgct agtccagtta tctatggtcc acaaacaatg gaagaattaa
    10981 cagatgattt gttaaatatg gttgtagatg tagcaaatgg aaaacaatca aaagcagagt
    11041 cccttggata tactgaaatg gcaatagcta gagtatgcaa ctatgtataa tctttaagta
    11101 agaaaataaa tacatagaat tttaataaag ggtgagatta tgaagtttgt aagttttact
    11161 gaaggagaaa atgaatgtaa aagaactgga gttttttcta aagatgaatt atttattata
    11221 gatgttaact ctttaaatct cagtagaaat tttaaggatt taaatgaatt aattcaaaat
    11281 gtatctagtg aagatattat taagttgaaa actattataa atgaaagtac attctataat
    11341 tatgatgttt ttaaaagaga gagatgtacg gtacatgttc ctatagaaaa accaatacat
    11401 gatatcttat gtgtgggagt gaactataaa gaccatcttg aagaaactca aactcacttt
    11461 gatgatagtt ttgaagaacc acaaaaaacg gtttatttta caaaacgtgt gacaaaggca
    11521 ataggtccag aagatgagat agatggtcat tttgagataa ataatcaact agactatgaa
    11581 gttgaacttg gagtagtaat aggtaaaaca ggtgttaata ttagaaaaga agaggtagaa
    11641 gattatatct ttggttacac tgttataaat gatatttctg ctagaaagtt gcaaaaagaa
    11701 catgttcaat ggtttagagg aaaaggatta gatacattta cctctttagg tccatgtatt
    11761 ttacataaaa gtgcagttcc ttttcctata aaacttaaag tatgtagtag ggtaaatggt
    11821 gaagaaagac aaagttctaa tacaggatta ttcatatcag acgtttcaga catagtttca
    11881 gaactttcaa aaggtatgac cttagaggct ggagacatta tagctactgg aacaccttct
    11941 ggagtaggga tgggattttc acctcctcga tatatgagta gtggagatgt agttgaatgt
    12001 gagattgaga gtattggaat tttaaaaaat tacattaagt aactgtttta atataattaa
    12061 tattaaagct aaatctgatg tattaatgaa catattaaaa taaattttag gagtttaaat
    12121 tatgaaagca gaatttttga caccagttgt tacaatattt gatgaagaag gaaatttaga
    12181 taaagaaggt aataaaaaag tgtatgacca tcttatagat ggaggtgtag atggactagt
    12241 cataatggga agtactggag agttctttaa tatgtctatg gatatgcaaa aagaacttat
    12301 agatttagta actagtcatg taaataagag agtaaaagtg tttataggta caagtcgtat
    12361 gtgtataaat gaatctatag aactttcaaa ctatgcacat aatagtggag cagatggtgt
    12421 tatgataata agcccttatt atttctctct tgatgatgat agtattgagt tattttatag
    12481 tgaaatagct aagaatacag aggctgaaat atatttgtac aattatccag atagaactgg
    12541 ttatgattta agtccagaac ttacattaag acttgcaaga aaacataaaa acatagttgg
    12601 atataaggat acagtaactt tgatgggaca taccagagat ttaataaata ctatgaaaat
    12661 agagttccca gaatttaagg tttatagcgg ttatgatgaa aactttatac ataatctaat
    12721 gtctggggga gcaggttgta tagggggatt atcaaattta attccagaag tttgctccaa
    12781 atgggctgaa gcttacaata atagagatat ggagaaggtt atagaaattc aaaaatatat
    12841 taataaagca atggattttt acttaataac caatccattt ataccagcaa tgaaaaaagc
    12901 tatgaatatc agagggttac aaataagtga ttatatgact gctccatttg taaaaccaaa
    12961 tgaaaaacaa gtatgtgaaa ttaaaaaatt gatgaatgaa ttaaatatat gttaaatcaa
    13021 ccttgcaaag aggtcaacaa taaattacaa ttatcttaat aaaaattaaa ttaaaataat
    13081 actagggtac tatattagtt taatagggaa agtgatgaaa attcactaca gcccccgcta
    13141 ctgtatacgg atacgaaatc aaaatccact gaaatttata aaaattttgg gaagggtgat
    13201 ggaggaaaag ccgtgagtca ggagacctgc ccagtattat ataagttcaa tttagggtgg
    13261 agattgtaat ttataataaa atgaagtatc aattcgtttt tgtaaagttg atatatgaac
    13321 ttctccttat taaattagga gaagtttttt attttattaa aaggttatac ttattaaaga
    13381 tgactttagg ataactttaa taagtatact ttaaaaagca tatattatta aagtttttta
    13441 gccactcata aaaaataaga tattggagtg aaagagatga aatttaaatc aaatgtttta
    13501 aagcagtttt ttttaattgc tatggtaatt tttatggtag gaggtcttat agggtgtagt
    13561 aaaagtgaca aagagaataa acaagctagt actggtaaga aattagaatt aaaatatgct
    13621 agtggattta gtgttgaaga tttagaagat ggaattaaaa aagtaactga tggagataat
    13681 cgagaactga ttttaatccc aaagaagatt aaaattcctg aaaaatataa aaatgctaat
    13741 atagttagaa cgcctgtgga caatgtatta atagcatcta caacacaggc ttgttctctg
    13801 agaacaatag atgaactaga tagtataaaa gcagtaacaa cagaagagca gtactggact
    13861 attaaagaaa ttaaagattt aatgaaagat ggaaaaatac attacgttgg ttcaaattta
    13921 gcaccagatt atgaaaaaat agtagactta aatcctgact taactatagt gagtagtggt
    13981 ttatcagagg ctgatacaaa atttattgac aaattaaaag aattagggct taattatgtt
    14041 gtagacaatg agtataaaga gcaaaatcca tttggaagaa tggagtggac aagtttaata
    14101 gctgcatttt atgataaaga agatatggct actagtgaac taaataaaac tgtacaaaag
    14161 gtagaagaag taagtaaaaa aataaaaaac aaagctaaac ctaaagttgt atgggcatct
    14221 gtatatgaag gaagtgctta tatagctcct aaagattcat atgtggataa tatgataaaa
    14281 atggctggtg gaataaatgc atgtgcttca atagattcaa atgaagggcg agtaagtatt
    14341 gaacaattgc atgaagttgc aaaagatgca gatatatttg tatactcatc aagttcagat
    14401 tatgcaccaa atttagattc tataaaatct agtgcacctg tattggctga tttggatgtt
    14461 gtaaaaaacg gaaacctatg ggtgtttgct ccagattatt atcaatcagt agataagaca
    14521 gacgaattaa tagtagattt agttgagatg ttccacccag gtactacagg tgaaaaaatc
    14581 aaacattata taaattatga taaatagtac agagaggaca caaaaagata ggttttatag
    14641 attttagtta atatagcttt gctaagagga gtatacaaat ggattttatc aataaaatga
    14701 gaataaggaa tacgacaata tttatagtat taattataag tatgatactt acttttttag
    14761 tgtgtgtagc atttggctct gtaaaaattg aatttaaaga agtaatagat gttgtattaa
    14821 gaggtatgga taatacagaa cattataaaa taatatacaa tataagaatc cctagagtac
    14881 ttgcaaccat ggtaggtgga gcatgcctag cagtatcagg tattttatta cagatatttt
    14941 ttaaaaaccc aatagttgaa ccgtatatat taggagtatc ttcaggttct actttatcag
    15001 tagctctcat gattttggga ggatacacat tgggatttga gagtgtaaat tcaatgagta
    15061 tatttgtagc agcttttata ggagctttaa ttgtaatgtc tatagtcatt ttatttgcaa
    15121 ataaggtaaa gaatataaca actcttttaa taataggaat tatgaccgga tatgtatgta
    15181 gtggtataac aaatgtgtta caatcatttg caaatagtga taaattaaaa gaatttacta
    15241 tgtggtctat gggaagtttt tctggattta catgggaaaa ggtagaaata cttataatag
    15301 ttggtatatt aggtcttttg atgactgtat tcacaataaa acctttaaat gcttttttac
    15361 taggagaaga gtatgcaaaa agtattggta taaatataag attttttaga gttttgatag
    15421 tatttatatc tagtatatta gcagcaatag tatcagcatt tgcaggacct atagggttta
    15481 ttggacttgc agtgccacaa atttcaaaaa tgatatttaa aacatcagat aatagagttt
    15541 tgataccagt agtaatattt ttaggcagta tagttacagc tctttgtgat ttaatatcaa
    15601 gagtaatttt atcaccagta gaattaccaa taagtacgat aacatcttta ttaggagctc
    15661 caattgtagt aatgttattg gtgaaaagga aggatagggc ttagacatgt tgaaaactaa
    15721 taatttaagt gtaggataca ataacaaagt tgttataagt aatataaatg tagaagtaaa
    15781 aaatggagaa atactatgtc ttttgggaag taatggagct ggaaaaacta ccctattaag
    15841 gtccttatct aaacttatat caccaataaa aggtgaaata tatttgaatg gtgtaaacat
    15901 aaattgtata tcaagaaaag cattatcaaa aaaaatggct ttagtactta caaatagatt
    15961 attaggtgat ttaatgactg tacaagatat agtcaatata ggaaggtatc catatacagg
    16021 attttttggg agtctatcta agaaagattt aataatggta gatgaagcat tggagtctgt
    16081 agatgcctta catttaaaga aaagatattt tgatgaacta agtgatggag aaaaacaaaa
    16141 ggtgttggtt gcaagggctc ttgttcaaga accagaaata attatattag atgagcctac
    16201 aactcacttg gatataaaac atagattgga actcataaac atactgaaaa aattaagtaa
    16261 agaaaagtca atatcagtaa tactttcatt gcatgaaata gacatagcat taaaaagttg
    16321 tgataaagtt gctcttataa aaaataataa agtcatagct tatggtcagc ctgaagatgt
    16381 agtagatgag gatattataa attcgcttta tgaattggat gataaaaact ttaatagtct
    16441 tttaggttca gtagaaatta gtaataaatc taaaaatgaa gtttttataa ttggtggtgg
    16501 gggtaaagca acaccaattt atagagcttt tactaaaaaa ggtataggtc tgtattctgg
    16561 aataatacat gaaaatgata ttgattatga aattggtaga actatgggga taaagatgtt
    16621 tacagaaaat ccatttgagc ctataagtga tgagagtttc gatttggcta ttagaaattt
    16681 aaatgattcc aagattataa tagatacagg atttagtgta ggagagacaa ataagaggaa
    16741 tatagatatt ataaaagagg ctttaaaatt ggataaaaaa gtttattctt ttagaaatag
    16801 agatgagagt aagaagtatt atgacagttt ggatagtaag atagaacata tagataaagt
    16861 ttctcaaata atcaatagtg cagatataaa taatttatta taatttatga aataaaatgt
    16921 acttatgtgt tatagaaaaa agagtataga ttataatttt tttgaaatta caatctatac
    16981 tcttttgtta ttttgtttat gtatttatat atttataaaa ctttagcatt ttatagataa
    17041 ccaatagctt cctctctagt taaaaaaaag tgaatccctg tagtcgattc tttccatcta
    17101 ttttcattaa aatcttcagc tataatcgtt tctcctactc tatatataaa attttcatca
    17161 gcatatgcac gagcactttt aaaagattct ttaccatcta cactagtaat tgacagtact
    17221 tttgccttat cacatctaca agcatttgat gtagcagaca ctctttttgc gtcgctgggt
    17281 attaataatt gtaccattct aaaattaaaa cattttttat agcctataaa tgctcctgtt
    17341 tcaggacaat acaatttaaa aaaacgggta ctttcatctg atattactcc atttaaagta
    17401 gcatgttcta aattggaatt ttctaaatta gctcctctaa tatctgagtt agataagtca
    17461 caaaatctaa aacttgcacc acttaagtct gcatttttta atatagaatt ttttaatgaa
    17521 caatccgaaa gataagaatt attaaaaata gagttttcta aatctgcttt ttctaaattt
    17581 aaattgataa ggtcagacca cgaaaagtca atattactta agcagtatcc tcttaagtcc
    17641 atattttcta atcttaattc ttttaaatct gccttttctc cagcattatt gtttttaagc
    17701 cataaataat gtttctcaag tgttttttct aactcttgtt tagaaatagt tttcattaaa
    17761 atcaccttca taaatttatt tttatatatg tagtagcaaa a
//
LOCUS       DACXMZ010000068        17686 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.068, whole genome shotgun sequence.
ACCESSION   DACXMZ010000068 DACXMZ010000000
VERSION     DACXMZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.068"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(91..1305)
                     /gene="dpaL"
                     /locus_tag="JD016_08915"
     CDS             complement(91..1305)
                     /gene="dpaL"
                     /locus_tag="JD016_08915"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HAU4999757.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(1928..2467)
                     /locus_tag="JD016_08920"
     CDS             complement(1928..2467)
                     /locus_tag="JD016_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HAU4999758.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            complement(2544..4349)
                     /locus_tag="JD016_08925"
     CDS             complement(2544..4349)
                     /locus_tag="JD016_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAU4999759.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(4745..5122)
                     /locus_tag="JD016_08930"
     CDS             complement(4745..5122)
                     /locus_tag="JD016_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAU4999760.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVRAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            complement(5537..5683)
                     /locus_tag="JD016_08935"
     CDS             complement(5537..5683)
                     /locus_tag="JD016_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999761.1"
                     /translation="MIIFWLLLYLAVIFSIGSMITRVNKVWVKVLLFIIVGLSTIVLS
                     YVIT"
     gene            6027..6512
                     /locus_tag="JD016_08940"
     CDS             6027..6512
                     /locus_tag="JD016_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999762.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(6702..7241)
                     /locus_tag="JD016_08945"
     CDS             complement(6702..7241)
                     /locus_tag="JD016_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAU4999763.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(7234..7647)
                     /locus_tag="JD016_08950"
     CDS             complement(7234..7647)
                     /locus_tag="JD016_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAU4999764.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKNMEAVSYE"
     gene            complement(7683..8804)
                     /locus_tag="JD016_08955"
     CDS             complement(7683..8804)
                     /locus_tag="JD016_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAU4999765.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLEMGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQENIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFVASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            9403..11055
                     /locus_tag="JD016_08960"
     CDS             9403..11055
                     /locus_tag="JD016_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HAU4999766.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKSELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKIENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            11354..12103
                     /locus_tag="JD016_08965"
     CDS             11354..12103
                     /locus_tag="JD016_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4999767.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYVASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            12116..14143
                     /locus_tag="JD016_08970"
     CDS             12116..14143
                     /locus_tag="JD016_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4999768.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFFYKTPLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQNSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            14287..15870
                     /locus_tag="JD016_08975"
     CDS             14287..15870
                     /locus_tag="JD016_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAU4999769.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPDVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKDI
                     IKLNINDNGVKNTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            15842..16462
                     /locus_tag="JD016_08980"
     CDS             15842..16462
                     /locus_tag="JD016_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4999770.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTTMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(16651..17577)
                     /locus_tag="JD016_08985"
     CDS             complement(16651..17577)
                     /locus_tag="JD016_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999771.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         6126 a         2757 c         2150 g         6653 t
ORIGIN      
        1 tttattttat ttaaagtttt cacttattta agtttttatt ttccttaaat ttttcattta
       61 tttgttaagt ttttcttttc cttaagtctt ttatttactt tggcattcac cattccaaac
      121 tatatctcta tatttatcag ggtcagtatc tccttctgta cttattaata atactttaga
      181 gttctcatct agtttcaaag cttctcttaa gtcttttaaa tcttcacttt caaggattga
      241 aactaatgct ccagttgtaa ctgctccaga ttctccagag attactttct catctcctct
      301 tagagggttt cctaatattc tcataccttt agcagatacc cagtctggtg ctgaaacgaa
      361 tgctgatgaa taatttttta acacttcaaa acctatagtg tttggttctc cacaagcaag
      421 ccctgccatt atagttggca tatctcctcc aacaaatctt ggttttccat ctccagcttc
      481 tgctgattta tagtaacaat cagcctcatc agcctctact actactgtta ttgggcattc
      541 atcatatatt gaagcaacat atccttgaac tgcaccagca agtgaaccaa ctcctgcttg
      601 aacaaatacg tgtgttggtc tatctactcc atattctttt aattgttcta tagcttcttt
      661 agccatagtt ccatatcctt gcattatcca tgctggtatt tcttcatatc cgtcccatgc
      721 tgtatcttga accattactc catggtcatc agcttctgca taatcattag ctaatcttac
      781 tgcatcatca tagtttaagt ctgttatact agcatctgca ccttctcctt tgattttatt
      841 taatctaaat tcagaagaac cttttggcat taatacaaca gatttttgac ctaatttatt
      901 agctgtccaa gcaactcctc taccgtgatt tccatctgta gctgtaaaga aagttatttc
      961 tcctaatttt tcttttattt ctttcgaagt caatttgtca taccctaact cagatatatc
     1021 tgtatttaat ctttgagcta agtatctacc catagagtaa gaaccaccta aaactttaaa
     1081 tgcgtttaag ccaaatctat aagattcatc tttaacatat attccgctta cgcctagtga
     1141 ttttgctaag ttatctaatt taactaaagg agtcttttca tattgtggaa agctctcatg
     1201 aaaagcttta gcctttgcaa tctcttcatc ttttaaaaat tcaattcctt tttctttttc
     1261 ctctgattta ggtaagttat ttaatttcca ttttatttct ctcatttatt ttatattccc
     1321 ctttcaaatt tttcggtgtc attttaattt tttatctttg tgccttatag caaagcaatt
     1381 accgtgccaa tattatttat tctaaaaaca atttttttat gttggaatta catactttta
     1441 taaaatttct ttaaagttta taaattttga gtgtgaaaaa taagatttat aaaagaaaaa
     1501 attctcatat tgggaaattt ttatcaatac gagaattatc aattcgagaa atcgtttatt
     1561 tatcttaatt cttcctttat tatgctctta ttttttatat gtctattgtt ttgtcagata
     1621 tattactact tttattttct ataaatatat ggctataacc ttcttacgca taaactctat
     1681 aaatttatat actttatatt ctttattacc caaatattct ttattaccca aagcctccaa
     1741 aatggcttaa atcttttcat agcttcttac agaagtttac atggtcgtcc ccccaataag
     1801 caaaagtgca atatgtgtac cattgtgccc tacttgcgac tattgagctg tcgaaattcg
     1861 caacttcgca tttatctgat gaaatgcact atacaaaata ttcataacat caacaacaga
     1921 gttaaagtta cctattcata ttcactgtaa aaatttcttc cacaggaaat gtaattttaa
     1981 ccaatcctat tgagacaaac tcaccactta tttttttgaa ctcacgcagt aatacatcac
     2041 ttgcccgaaa aaaagcctca ctttcatcat ctccagtaag tgctattgtg caatggggta
     2101 cccaacgatt ggggtggtac cattcccaac catttgaatc aaaatcttgt agtacctcat
     2161 ataattctct ctgaaactga tacatactgc tattcattat aggtgataca aacaccgttt
     2221 tagtatcatt aaacatgcct actgaaccaa tgtaagcagg tattacctta tggttttgtg
     2281 caaacccctt tagcttgtct atgcaacaag cctcatccac atcattgaaa caagccaaag
     2341 taagatgagg tcttgttttc cattctaagt acttcgtact aattttttca tcagcgattt
     2401 ttttagataa atcaaaaagt tgcttttccg tttttttatc ataatatagc tctatcgcat
     2461 attgcataat aacacctcca ccataaagta aaaataccta tctataaaaa cacacaaata
     2521 cctatcattt taatattata ttactactgt tcctcttcca attttctata tagtgtagcc
     2581 aaacctatgc ctaagtcttt tgctatcttc ttcttcgtct ttgtatctct tccatactta
     2641 ttcaacaact tgtttatgta agtcaactct aattctttaa ttgaaagtat cctttcttga
     2701 ttctctacta tccctccaac tatatcatct tcaaatatta tatttacatc cttattttta
     2761 taaatatttc cattaatatt ataatagttt aaaatatcta atggaagcat atctttatat
     2821 attattccat catctccaac caaattcatc atatattcaa ctgcattttc cagttccctt
     2881 atatttcccg gccaattata gttaatcaac atattcatta cattttcttc tattttattt
     2941 aactgaatac ctaattctaa agaatattta gaagcaaact tcatcattat tgcctcaata
     3001 tcatcctttc tttccctaag aggtggcaaa tttataggaa ttacattcaa tctatagtat
     3061 aaatcatccc taaacttacc ttcatttact aatttcaata agtttttatt tgtagcagca
     3121 atcactctta tatctaaatc tataagttta tttgacccaa ttctcgcaaa ttttctctct
     3181 tgtaaaactc taagtaactt tgcttgtaaa ggcattgaca aatctcctat ttcatcaaga
     3241 aatataacac cttcatttgc aagttcaaat ttacctactt tccctccact actagctcca
     3301 ctaaatgcac cttttatata gccaaatagc tcactttcaa ggagattttc tggaatagca
     3361 gcacagttta tcgcaataaa tggcttgttc catctgctac cttcagcatg tatagcccta
     3421 gcaattagct ctttacccgt accactttcc ccagttataa gtacagttga atttgacttt
     3481 gctaatttct tagttctctc ttttactttt aacattgctt ctgaatttcc aattatagac
     3541 tctatatcac tatttcccca gccactattc acttttccat gtcctttatg attgatatat
     3601 gtcttgtcaa atattattat tttgtcgtat tgatttatat atggatatac cggaattatt
     3661 ttggcattta tattataaat tttgttatca atatcaattt ctagtaattc atgtccattt
     3721 gaggaagatt caacagatac tatatttact atattttcta ttatattttt atctatatct
     3781 aattttttta gagctatatt gtttatataa gatattttgc tatttatgtc tagtattata
     3841 acgccttttt caacattttc aatgatttct atcatcatat ccatttttct actgctattt
     3901 tttgacgctt catgttcttc tattttcata gaaattaaat cgcatatatg attgacaaat
     3961 tttaaataat tatctatttt tgcacttatt tctacttttt tagttttatc tgtactaata
     4021 gcaccaatta ctcctatagt tcgattatta tactttattg gaactgctat tattacttta
     4081 tttaagcatt tcatgtaatg ggaacattct atacaaagct gattatcaca tatatccaat
     4141 acgactaatt cctgacctgt ctgtataaca ttgtcataaa taaatccttc tgtaacaacg
     4201 ctttcattta tctttgattt atacagacct gtaccactaa ttcttattaa tctatcgtca
     4261 actatttcaa tatctacatc taacacacta gctactgttt cagtgtattt tatcatttct
     4321 tgttgaaatt cttttaattt acttaacata gtttccccca ttagaattta atttaccaaa
     4381 ttatcagttt agctatattt taacatatag tcactcttta aaatatatat aaaacattac
     4441 ttatttatat atttgcgtta aaacagacaa tattaattta taaaaagttt gttttatata
     4501 gtaaccttaa ttataatatg cagacaataa aataaatcac aatacaataa taaaaaaaag
     4561 ctttcctcat ataacaaata tatttttgac ataaaaatat acattatatg agaaaagctc
     4621 aatagttaaa tatttagata gatttttata tagacttttt taatataaac ttttttaatt
     4681 gtagactttt tttattagtt tttaattttt attagttttg ttattggttt tattgttata
     4741 atacttataa tactgccatt gcttcaattt ctaaaactgc atcttttggt aattgagcaa
     4801 cttgtacgca tgaccttgct ggtggatttt ctttaaaata tgtagcatat acttcattaa
     4861 tatcattaaa atcctttaaa tcttttacga atactgtagt cttaaatatt ttatccatac
     4921 ttgtaccagc ttgttctaat attgctctta cattatctaa agaagctttt gttgcttctt
     4981 ttgggtcaga tactaactcg ccagtcttca tatctattgg aagttgacct gatgtagaaa
     5041 ctatgtttcc aacttgaatt gcttgagaat atggtccaat tgctcctgga gctttctctg
     5101 tagctattac tacttttgac atgacttcac ctcctattat taagttatat ttacaggaaa
     5161 ataaaatttc ctaaacacca atattaatac gaacctttag attaaatttc aaatctaaaa
     5221 cttcaaataa tcaatactta gatgattatg tttatttgtg tcttgttaac ttttattaaa
     5281 gcaattaacg tgccaaactt tatttatata taaaattttg tgaagtcatt caaaatagtt
     5341 acttctgaat tttttcgtaa aaaaacgctc taaaattact tttttatgat gtcaattttt
     5401 ttctcatatc tagaaattta ccaacatgag aattcttgtt ttgagaattt acagattaaa
     5461 gttatttttt aaccttaaat tctgaacctc ccatgactaa agtcacaggg ttcctgcttc
     5521 atagaatttt gcataatcaa gttattacat atgaaagaac tatagttgac aatcccacta
     5581 ttataaataa taaaaccttt acccatacct tatttaccct agttatcata gagcctattg
     5641 aaaatataac agctaaatat aataataacc aaaaaattat caatatctac cactgttgct
     5701 gaaaatccaa aaaactacct tttgatagta ttacttaaca atctaattaa tctcataata
     5761 aatttacaac ttataaataa attgttatat tggccgtatc tcaacaactt tctcctttcc
     5821 tagttttaaa tcttttatag aattgtacca cgtctaagta aattttcata ctattaagtc
     5881 ttatttctaa taaatcatta gctctcattc tatgcccaat aatgtaagat tgcagtaatt
     5941 ttattgattt taatagtttt atttaatata cttatattaa ttattttcat atgaaattat
     6001 tatttatata gggggatttt tttaatatga gcaaaagtga gttaacagca gaaacaacag
     6061 aagaaatgtt agaagtacta agtggtaaag attatgatat tgcatgtcat ttacatgaac
     6121 ttggtaaatc attagattgt aaaattgaac caaaaacagg tgctcgttct tacaaaatag
     6181 tatattcaac taagaaacca aaacgtagct tatttactat tgaatgtaat gaaaaaaaat
     6241 ggagagttaa agcaaatctt tttcatctaa atacatataa agatgctgtg gaggaatgct
     6301 ctaaaactat taaagatagt ataactaaaa ctcgtacttg taagaaatgt aattcaaagt
     6361 gtattggagg ttcttcgttt gaattagatg gaaagtctta cctgacttgc ataggaagtg
     6421 gtcattattt tgcaaatatg gaagaaatgg attggaaaaa cctagaaaaa ttaattacta
     6481 aagaaaataa tattatgcag gaatctgtat agttttttaa gggtaattat taaaatatat
     6541 gagtaacaac agatacttaa ataggcataa aaaaataaag ggtataataa ggatatagaa
     6601 taaaaataaa tgatatagca caaagaggct gtctcaaaac acagataagc ctctttttat
     6661 tgttgaaatg ataagaatat attcaatttt atacaaacat actacgccaa cttacccatt
     6721 tcatatgcct gtttcattgc cttacttccc ttaatatcac ctacattcca agcacctaca
     6781 ccatatacta tacctttttc cttagagcca tttagacatg atgtaaatcc tctaaatcct
     6841 tctattgttc tttccatatc agccacattt tcatctgctg ctgtaacgat aaaatagaaa
     6901 tctttatcac aaatttcagt atatctagag caagttctat caatcatagt tttcatttgt
     6961 gcattcatag tataaaaata aactggagtc gctaaaacaa tcacatctgc cccaatcatt
     7021 ttttcaagaa tttctcccat gtcatctgtc tgtgaacaat ttgcttcttt ttcataacaa
     7081 gcaccacatc cagtacaata gttaatatct ttatctttta aaaatatctt ctccacttga
     7141 tttttagatt ctaatacccc ttgtataaac tggtcacaca ataaatcaga atttccacct
     7201 tttctaggac tagctgatac tattaataca tttttactca taagatactg cctccatatt
     7261 cttatttcta ctgctcttta atactttttc tttctcaata ataccttctt catatctatc
     7321 aatcttaaaa tttaatcgtt ccaaagtttt ttgcatttct tctattcttt ttgctagttg
     7381 gtttctctgc tctattaaaa gttcttttct agctttgata gtactgctcc cttgttgaaa
     7441 catagctaca tactcaatta atgtctcaat agaaagacct gcacctctca tacactttat
     7501 gaaattaacc cactcacaat cttcttctgt aaaagaccta attccacttt tatttctatt
     7561 tacaggtggt attaaaccaa ttcgttcata atatcgtaaa gtatccgctg aaagctcgta
     7621 ttttttacta acttctgaaa tcgtcataca tatcttctcc tcttgatatt gtatagattg
     7681 aattatttct caaaggtttc tgctacattc tttaaagcat ctataatttt aatgtgttga
     7741 ggacagcttt cttcacattg tttacattca atacaatctg aggcctttcc ataagtttta
     7801 gtataattat tatagtagac catctgtgtt gaaaatccag tattaagagc ttgtttttct
     7861 gcattgtaca acgcaaaata gtttggaata gcgatatttt ttggacagcc ttctacacaa
     7921 tactgacagg cagtacaagg aactaaaatt gattcgttga taatctctac tgcttcatct
     7981 ataatattat attcctcttg tacgaatggt ttgaaatttt gcatatatcc tgtattgtct
     8041 aataattgtt ccatatttga catcccactt aagaccatca taacattatc tttacttgct
     8101 acaaaacgta ttgcccaaga tgaaattgac atatctggat tgtagtcatt caataatttc
     8161 tctgcttttt ctggtatctt tgcaagtgta cctcctttta ctggctccat aacaattaca
     8221 ggcttattat gcttctctgc cacctcgtag cattttctag attgtatgct ttcattatcc
     8281 caatctaaat aattaatctg taattgcacg aagtctactt ctggatgctc actcaagact
     8341 ttatccaata attctgcact atcatgaaaa gaaaaaccaa tattctttat ttttccttct
     8401 tttttctttt cttcaataaa cttaaagcta tcaaattttt tagctacttc ataatgtgac
     8461 acgcctatat tgtgtaaaag atagtagtca aaatagtcta caccacattt ttctaattgc
     8521 tcattaaaaa tgttttcttg ctcttctttt ttctttaaag ccattaatgg aagttttgta
     8581 gctatagtaa aactctctct tttgtgtctt tttactagag attctctaag tgctatctca
     8641 ctttttccca tgtgatacac ataggctgta tcaaaatatg taaatcccat ttctaaaaaa
     8701 gtgtctacca ttttatttaa atgttccata tcaatagttg taacatcatt ttcatctttt
     8761 attggtaaac gcattaatcc aaatcctaat ttttttgcct ccattgtttt acttccccct
     8821 ttaaaacttg tttgtttttt ctctctactt taactattct tacaagtata taattcttgt
     8881 ataattatac tttactactt ggagttaact tcaagtcaat agattttttt atttttaaga
     8941 caaatattat aaaaatacag ttctttaata ttaatccatt acatgtttct ttcaccctcc
     9001 aattctcaaa cttcacaaac tccaacatgc aaagaaatat atactttaag aaattcagtc
     9061 cattaatatt actacaaata ctttttagat atgtttaaaa agaaatttca cctaaaatgt
     9121 ttataaaaga tttagaaata ggtcttgtgt ctattttaaa atatataaaa cagtggtaaa
     9181 gacttattta taagataaaa aatgaatagt gtataccctt tttaaaaagt tttttccttt
     9241 ttatatttgt gatacaatgt taaatataaa aaggaaaatt ttgtattaaa gaaatcttat
     9301 ttgttgattt tgtaacttca aactcagcat caatttgatt ttaagattat attttattaa
     9361 gtatgctaaa taacaaattt ttaaagattg tgaggtaaaa atatgaataa gaaaaattta
     9421 gctatattaa tatcaggtat gatgtttttt tctagttctt cagcaatttt tgcagacaat
     9481 actacaaagc aagaaagatt aataggtaaa acaagatatg aaacagcagt agaagtaagt
     9541 aaattgggat gggttcaatc aaaaacagcc attatagtta atggtaattc tattcaaagt
     9601 gcgctttgtg caaatccatt tgctaagcta aagaatgcac ctatactttt agtaaacaac
     9661 aacagtattg aaaattcaac taagtctgag cttaaaaggc ttggtgtaga taatgtgtac
     9721 attgtagata gtggaaatag catttcttct aaagtagaaa atgaaataaa atcacttaac
     9781 ataaaaataa ataaaatagt aggaaacaat atatatgaaa tgtctacaaa tgtacttaaa
     9841 gaaatagata agataaaaaa gatagaaaat gttgcagttg taaatggact aaagggacta
     9901 tctgatgcag ttagtatagc ttctccagca gcaatacata atatgcctat aatattagta
     9961 tcagatgaaa acagcaatga tagtagtata aatttcttaa acaataggtc tataaaaaaa
    10021 tcttacatag ttggaggaaa taagatttta tcagacaata cagtaaagaa atatccaaac
    10081 agagaaagaa tcagtggttc agatagaaac aatactaatg caaaaattat tgataaattt
    10141 tataaagata aaaaaataaa taatgtattt gtagctaaag atggtattaa ccaagaaaat
    10201 gcattagtag attcattatc aataggagta ctagctgcta aagaaaattc acctataatg
    10261 atatctaatg gtagccttgt aaatagtcaa aaagaattta ttaaaaataa agatatagat
    10321 gaaataacac aagtaggagg aggatataat caaaaagcat ttgaagatat tttaaaacta
    10381 aaaaatgtga acataagaaa taaaactaaa aaaatatctt taatacaggc taaagaaata
    10441 ttaaaacaag ctcttccagg aagcaatatt ttagtttgtt attatgatga agatgataat
    10501 gaatatgact gcaaagttat aaaggataat atcctatatg agatagaaat tgatgcctac
    10561 tctggtaaaa taatagaaat tgaaaaagat gatgacttaa atgataataa tttaggtaca
    10621 caaaatatat cattggataa agctaagtct atagcattag atgctgttcc taatggtaat
    10681 ttaatctatt gtaaatatga tgctgaagat aacgaatatg aggctaaagt aataaaaaat
    10741 aagaaaacat atgaattaga gataagtgca tttgatggaa gaattgtaga tattgacatg
    10801 gacaatgatt ctgatgatga ctttgatgat aatgataaga ataactctaa tactaatgac
    10861 aacagttcta ttattagtgc tgaaaaagct aaatcaataa tgctagataa agtgcctggt
    10921 gggaaaatag taaaatttaa gtatgatgaa gatgacaaag aatatgaagg tgaaataata
    10981 aaaggaaaaa ctgaatatga gataacaatt aatgcaatta ctggtaaaat attagaattt
    11041 gaaatagatg attaataaaa aatatatatt ctattaatta ttacaagcaa taaagccata
    11101 aatattgaaa ttttcaatat ttatggcttt attttatttt ttttaattta aatccacatt
    11161 atgccattat atatatctat gcactctttt aaattaaaat cttgtccatc atcccaaacc
    11221 taataaagtt agattttaaa caatatattg tgatataagt aatttgtgta gaattattgc
    11281 gtgatttact catgaagaaa caataaggtt aatatataat ttgactatat taagacgaaa
    11341 ggagacatat acaatgaata caatattaaa agtaaataac ataactaaaa cttataataa
    11401 agggcaacag ccgagtttag ataaggtatc ctttaatgta tcagaaggtg aatttatagg
    11461 tataatggga gcttctggct ctgggaaaac aactctactt aatgtattat ctacaattga
    11521 taaaccaaca agtggagata ttttcataaa taatataaat ttgaaatcgc ttaatgacac
    11581 ttgtgctgct gattttcgta aggataattt aggctttatc tttcaagagt attttttact
    11641 agatagtctt acaattttcg agaatatttc agtacctctt acacttcaga aactcaatcc
    11701 agataaaata gaaaacatgg taaagagtct agctgaacga tttggcatag cttctcaatt
    11761 aaataaatac ccaaatgaat tatctggtgg gcaaagacag agagcttcag cagttagagc
    11821 aatagtaaaa catcctagta ttctctttgc agatgaacca acaggagctt tggattctaa
    11881 ttcagctact gaactactcc actcactaca ggaagcaaat gaggagttga aaacaacaat
    11941 tttaatggta acacatgatg cctatgtagc aagttttgca aatagaattt tgatttttaa
    12001 agatggtcat atcattaaag aattaaataa acaacagaat aatagacata cattttatga
    12061 gttgattatt caagaaattg caaaattgga tactaaaaga taggaggata atttaatgaa
    12121 cattttttat acggcaataa agaatcttaa aaaaaacttt tcattctatt ctctatatct
    12181 actttcagtc tcttttgtta ttactgtatt tttcgcattt acttcatttt ctgttaataa
    12241 gataatgcta gaaaaaattt caactgatgg tagagttgaa tctatgtgta atgttatttc
    12301 gatatttttg atggcatttg tcatcttcta tatggcatac tcaaatcgat tcttcttgag
    12361 acgtcgtaca aaagaattag gtatatatgc tttacttggc tatcgtaagt ttacaatatt
    12421 atcgctactt acctttgaaa atgtattaat ttgtagtgga gcttttatta ttggattggt
    12481 attaggagca ttggcacata agggtattgt ctttggaatt acagcactac ttaatttatc
    12541 aattgataat tcacagatac cattttttaa tataaatgca ataaataaaa caattatctt
    12601 tatagcatta gttgttttag tcttagctgt atcaaatggt aaattttttt ataagactcc
    12661 acttgtaaaa ttgataagat atgaaaaaag tgcagaaaaa aaattaaagt tcagaaaaat
    12721 tccagccttt atggggttta ttatgatttt aagcgggtat tgtttatcac tagatattct
    12781 tcgtggagct aaatcagtat gggtaagtat tggattttat caaatgggct tgataactat
    12841 gattatcatt gtaattggta cagttctctt tatcaattcc tttcttccat atattatgca
    12901 aaatagtaaa aaaaataaac gtactttcta cacagatatg aagataatag ctattccaaa
    12961 ctttatctat cgtatacgtt caaactctaa aactctaatt atgcttacat tactctctgc
    13021 tactactttg acagtttcaa gtgttatggc actgtcctta tactacccta ttgcatctgt
    13081 ttcgcgtatt gcaccttcag agatagaatt tagagtagaa aaaaataaac aaatagaaga
    13141 tgtaaaacaa attgtcagtc aatatacgtc taatacagat gatattattt ttacacagac
    13201 aaatatttat aaggtaaaat cttcatcaaa agaccttcca tttgaatatg aaattggtac
    13261 atctaagaat aatttatcca atagtagttc ttctaaagaa gttatttcaa gagaatcagg
    13321 atttgaatgt atatcttttt ctgattatta tactttatta aaggcacaaa atcgaacaaa
    13381 gctactaaaa caacttaccc ctttaaggga taatgaatgt attttaatga aatatcaacc
    13441 aaataatgat ggatatagtg aaaatggaac gatttatcca ttacttgtta atggtaaaga
    13501 aacacctgta gctgtaaggg aaacatcatt aaacaatgct atttcttttg ccaatagcgt
    13561 tggaacactt atcgttaatg atagtatata tgagcaaata gcacaaaata aattaccaat
    13621 gacaagcatt ttaagtatta atggtaaatc aattaaaaat aatgaaaatt tatatagtaa
    13681 tatttcagat tatttagatg gaagccctta tttacaagga tattctcaca ggataaatga
    13741 tttgttatca attaatagtt ctacatttct gttaatcgga tttcttgtgg tattattctt
    13801 tattgctgtt ggaagtattt tatactttaa caatatatca gcaatttcag attcaacatc
    13861 tgattataag attttacaga aaatgggtta tgataatata caaattaaaa aaattattaa
    13921 aaaacaagta ttgccatttt ttaatattcc gtttttactt ggtatattgg actgtatatt
    13981 tgctaccttg gtttataaga ctgctttgat gcagaattta cttggaaatt cattttcaca
    14041 atacatacct gtgattattg caattttctt aacttctgta gtgtatttga tatattatat
    14101 acttactatt aaatcctgtt gtaaaattgt attaaaaaat taatatttac caaatcgtca
    14161 agcttagagg tgtgacgatt tggtatactt catttataag tatttgatat aatttaaaat
    14221 tatagattct taggtttgat ttagtcataa aattaatata attaactcat aaggaggtgt
    14281 ataagattga caaattttaa tttaaaaata ctatttttag gaaactacat tctatcactt
    14341 ctcttcatgg gaatatctac aagcactgaa acttatatga atatccaaag cattattccc
    14401 ttaataattt caacattatt actcatagac atcacatata taacctcttg taatgtccga
    14461 gaaaataaca ttatttcatt gttttgtgga ttactagcac tagacagttg gtatttactc
    14521 ttatcatttg taaccacacc aataggcact attattttta aaatattaag cacagttata
    14581 gtgtatgtct ctattaaatt tatattttta tttttgtttc aaggtagtgg atacaatttc
    14641 cgtaagataa cagacatttt attgcttatc acctgcattg gtgcaattgt aggagcattt
    14701 atttctaaca gaacgtatgc ttgtatgtac ggaatacagt ttgttgttag tataataagc
    14761 tttttctata ttatttttta tcatttgaaa agggttgttt ttgtcataaa aagcgaaaaa
    14821 aaacctatat cgatttcagt atttataaca attataggat tccttactta ttattttttt
    14881 acactaagaa tatcaaatca tattggtaat tttggaatct atctaactgt actggtattt
    14941 tttatgagta ttcatggtat tgcacttaaa gaaaatgata gttttcctat ttcaacagtt
    15001 tttaacataa aacaaagggc tatatttctt tttataagta ttttagtaat tagcttggtc
    15061 gtactatttg ccaatgggaa tttttctata atattaatag ctataaatgt tttatttatg
    15121 ctcattttcc tctgtaacat tattttggag tttaacctta gagatgaaaa aaataaagta
    15181 gttaaaaata gcaagtatac ttttgcatta gaaaaattac aacaagaaga acaactaaaa
    15241 acagagtttt ctaatttttt acatgatgac atattacaga acctgctttc aataaaaaac
    15301 atgatgagta agtcatatcg cccagatgtt caagatatta tatatgaaac acttaacaat
    15361 ctaaatacat gtataaggaa gcaaatgcaa gactatcatc ctgttatttt aaaaaattta
    15421 acagtaaaag aaaattatca gaatttaatt gaaagtgttt cttcatcctt tccacaaaaa
    15481 aatatttgtg tttcatttaa gtgttctgat actttattct tagttgaacc atatgatata
    15541 ttgatatatc gactgattaa agaattattg acaaacgtat ataaacattc aaatggtagc
    15601 catgcatgga tagctttagc actagaaaaa gatataataa aattaaatat taatgataat
    15661 ggtgtgaaaa acacatcttt taaaacacct ttagatataa ctaaaattac ttttgataca
    15721 ataaaacaca aagggattat atcaattaaa gaacaagtgg aaaatctagg aggaacgata
    15781 agtatttctg ataatatacc tcatggagtt tgtgtacaga ttacaatccc tatgaaagga
    15841 gatggttctt ataaatattt tgttagttga tgaccatatg ctttttgcta aaagcttgtc
    15901 aattgcacta gaagaataca aagaaatcga acagttttat tccacacagg acatccataa
    15961 tctttcagaa atgattcaag ataaaaaaat agacattgtc ttaatggata taaaccttag
    16021 aggcttaagt gaatgtgatg gcttaaaagt agcttataat ttattagaga ctaattcaac
    16081 tattaaaata ataatcttga ctggatacga cctcccagtt tataaacatg aggctaaaaa
    16141 aataggtatc tatggattcc tcaataagaa tattagccct gatagtttaa ttgattcatt
    16201 attaaaagtc tatcaagggg taacttgttt tccatctgaa aatactgaag taattattga
    16261 agatttaact actatggaga aaagaatttt acagcttatc tgtagtggta aaaaaagaaa
    16321 aataattgct gatgaattgt atataagtga aagaacatta tcaaatcatt tacaacacat
    16381 atatgacaag ctagatgttt catcatcagt tgaggctatt acaaaagcta ttaaaatggg
    16441 ttatatttct ccagtatatt aaaaaaagtg agtgtctcaa aataaatctt ctaactcata
    16501 aagcactctc ctttgtatta aatcaaatat attttcatca tttaaacaat taagaagagg
    16561 cttctctcta ttttgagaca actccatttt aatacactat ttatatttat atataaacat
    16621 attttaaaca actaaaaagc aactaatatc tcaatacata tcttcataac acatatcacc
    16681 ataattacaa gcagataaag aactctcctc atttaaatac gactcaaatt catacccaaa
    16741 cagaattgtt ggttctatat atatttccat atctgtatat ctccatcttc ttacctttgc
    16801 ctctataacc ttgtaaaaat cttctaaact atacccttct tttaatcttt tatctataag
    16861 tgccttagtt atactcaaat cagctttaaa attttttcct gattcactat ttaattttct
    16921 tataataatt tcatgtatgc tttctggatt tttgtaagta gaatcttcag tcaaatcttc
    16981 atccgaattt tcaaaggtat ttttatttaa attcttttta tttaaattat tttttaataa
    17041 ttcttttttc tttgtctcat tttctgtttc atacccagta tgaattatca aatcataatc
    17101 tttgtctata ctctcttgat ttatacggct gtatatgttg gtattactgt tcttaaacaa
    17161 ctcatctcta tgtttatatt tcattgtttt ttggttattg ttaatagttg catcaatgta
    17221 tttctctata tttttgaaaa tatcaccatt ctttgttaca atttctgcat tactactttc
    17281 agaacaaaca taactatata cagaacaaca atttcccttt actcccctac ttattaatct
    17341 caatatacct aaatctataa attcatttac aagtctactt attgtagata tagatacatc
    17401 aaggtcagat gcagctttcc ttactgtcat cataaattgt cctcttggca gagttccttt
    17461 taatctttgt atattttctt ttctcattac atagtgatta aaccttattc tatttacatc
    17521 tcttttttca ttaaaatata atactgctga atttaattga taaaaactat tactcatatt
    17581 cccactcctc caatttaaat agataatttg ttgtaaagtt tttagatttt taaataactt
    17641 attaaatgat tatttttatt aaaaagtatt ctttgatatt ttcatt
//
LOCUS       DACXMZ010000069        17283 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.069, whole genome shotgun sequence.
ACCESSION   DACXMZ010000069 DACXMZ010000000
VERSION     DACXMZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.069"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            34..780
                     /locus_tag="JD016_08990"
     CDS             34..780
                     /locus_tag="JD016_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAU4999772.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKFYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            841..1515
                     /locus_tag="JD016_08995"
     CDS             841..1515
                     /locus_tag="JD016_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HAU4999773.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            1883..3229
                     /locus_tag="JD016_09000"
     CDS             1883..3229
                     /locus_tag="JD016_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999774.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQMTLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKSINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEKAYSIFYGDILESCNIVLMNSNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGVNTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3256..4650
                     /locus_tag="JD016_09005"
     CDS             3256..4650
                     /locus_tag="JD016_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999775.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GESGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDIKMHKPEVGDIISTIIKDKSILSAHYGGTWEKIGEKI
                     EFGITVHYYKLIEK"
     gene            4860..6296
                     /locus_tag="JD016_09010"
     CDS             4860..6296
                     /locus_tag="JD016_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999776.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKDMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGKETVYYYKHHEPEKTNQIQ"
     gene            6640..7584
                     /locus_tag="JD016_09015"
     CDS             6640..7584
                     /locus_tag="JD016_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HAU4999777.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGITLEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEGNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7753..8685
                     /locus_tag="JD016_09020"
     CDS             7753..8685
                     /locus_tag="JD016_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAU4999778.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKVK"
     gene            8790..9257
                     /locus_tag="JD016_09025"
     CDS             8790..9257
                     /locus_tag="JD016_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HAU4999779.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9501..10355)
                     /locus_tag="JD016_09030"
     CDS             complement(9501..10355)
                     /locus_tag="JD016_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar uptake protein"
                     /protein_id="HAU4999780.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYMENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10515..12485
                     /locus_tag="JD016_09035"
     CDS             10515..12485
                     /locus_tag="JD016_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAU4999781.1"
                     /translation="MKMLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKS
                     LKEVEVVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVL
                     LKSNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDK
                     RQLTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRE
                     RKFEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKF
                     SILLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETA
                     YDKDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDI
                     RINDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIE
                     KVLSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTS
                     KKYKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYT
                     SFQSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEV
                     FYDFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            12485..12946
                     /locus_tag="JD016_09040"
     CDS             12485..12946
                     /locus_tag="JD016_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAU4999782.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            12949..13269
                     /locus_tag="JD016_09045"
     CDS             12949..13269
                     /locus_tag="JD016_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAU4999783.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13270..14385
                     /locus_tag="JD016_09050"
     CDS             13270..14385
                     /locus_tag="JD016_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAU4999784.1"
                     /translation="MEGNVMLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGS
                     FYNIPDIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPG
                     LVGGMIAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLIT
                     VVLINYVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTS
                     LALLTSGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSE
                     GAIPFAVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSI
                     AVGTVIVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14505..17105
                     /locus_tag="JD016_09055"
     CDS             14505..17105
                     /locus_tag="JD016_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAU4999785.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNVFIEEPGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLDKSNDTFISNHNYKIIYEDGSLNLQLKDGTYIS
                     NFLILKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPVDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6855 a         1791 c         3005 g         5632 t
ORIGIN      
        1 gaaaaatgta aattgtgaaa tggaggtagc aacatgaaaa ttgaaaatct ttctattgat
       61 gatattaaac gaggctatat atttaatgat gatttaaatt gttatagttg cattaaatgt
      121 acaaaaacat atgaggtagg agaaatatat aaaataggta atagattctt tgaagcttca
      181 aaagctattg aacttcatat ggaattagaa catagtgatt atttagagca attgcttaat
      241 agtgattcaa aatacaacac attgactgaa aatcaaaaac aattatttca attatttatt
      301 agtgatttac cagataagga gatagcaaaa caggttggag tatccacatc tactatacgc
      361 catcaaaaat ttattttcag agaaaaagca aaacaggcta agttctattt agcactatat
      421 gaatctgtat ttgaagataa atctaataaa agtagtacta taataccaat ccatgaacat
      481 gctcgaatgg tagatgaaag atatgtagta actgaagaag agagaaaaca cattttggat
      541 acatcatttg aaagtatgaa tcctttaaaa ttaaaagtat tttcttcaaa agaaaagaaa
      601 aaggtcgtaa ttttatctaa gattttggaa caatttgaac gtgggaagaa atattcagaa
      661 aaggaagtta atcagatttt agaatctata tatgatgatt ttgcaacaat tagaagatat
      721 ctaattgagt atggttttat gagtagaaat aaagaatgta caaaatattg gttaacataa
      781 ttaaaataat aaattttact aatgtgtaat gttattttta tctaggggga ggagtcaaat
      841 atgaatgatg aattaaaatc tattttatac aataaaggtg tagacattgt tcgctttgtt
      901 gatatatctg aatttccaat aaatcaaaca caaggttttt ctaaagcaat tttattttgt
      961 atagggttat caaaaaaatt tattacagat atatacaata acttacctac agatagtgat
     1021 gagtttttgg aaaaagagga gaaagtagaa gaattagctg actggatttc aaaatatatt
     1081 caaaataaag gatatagagc gtattctcaa tcggagaaaa acaatctaga acatggatat
     1141 tttgaaaaag cctatattaa tccagaaatg caatcaggga ttagtccttt accacataag
     1201 acaatagcaa atattagtgg cattggattt atgggaaaaa ataatttgtt tgtaacagag
     1261 gaatacggat gtgcttttag catgtgtaca gtacttacag atgctccaat ttctgttgaa
     1321 agatatcctt tgattgattc aaaatgtatg gattgcaatg tttgtgtaga gaactgtcct
     1381 gcaaaagcta tacatggaaa tgaatggact ttacctggaa aaagagaatc tattattgat
     1441 gtttcaaagt gtttttgtgt attgaggtgt atgatgagtt gcccatggtc attaagatat
     1501 gcaaatcaaa aataacttta ctagagaagg tattaaattg aatattataa aatttttaaa
     1561 atgtaagatt atataagatt tctcaaaata aaaatttgta tagaacactc agaaatgagt
     1621 gtttttctat tgggattaaa ttatattgat acaaagctta actattttaa tcattatata
     1681 aaatttgtat taaaaaatta agaataactt ggtaatagtg aaaatataat gagattataa
     1741 ttgatttata atataactat aatgatattg taaaatgaaa attgtcattt caaaattccc
     1801 aaaaagagta aaagtcactt cggttatagt caaaaaaatt tttctgtgaa aagaatttag
     1861 atttaaaagg gaggaattaa ttatggaaat tgaaactaaa actccggatt ggattaatgt
     1921 actagaatta aacagtaaaa tagatattac tgggaaatat gatgtaagta atatgataca
     1981 agatattgtc tctgataagt cactagaagg ttccattata tattttccaa aaggtaatta
     2041 tttatttgag aaaggtatta aaattagtca acaaatgaca cttcaaggag attcttatta
     2101 tggaggagat aatcaagtat caaatttaga taaaaaacaa tcaatcatag gaacaaccaa
     2161 ttttataact cgaggagttt ctaatatgag tattattgca ttgacaggaa caagtcaatg
     2221 tatcaaaagt attaattttt attatgatag tcatgatata gagaaaatac cgcctaaaaa
     2281 tgtatctgct attacagaat atggagaaac acaaggacta tctcattttg aacacctgtt
     2341 tatttctgga ttttcaggga tagggattga aattccatac tattcaactg gcaatgatat
     2401 aattgttagt tcatgtggtc ttggtatgag gcttggagag aagtcaatgc tttctagtag
     2461 caagatttac gaatgtaaaa atggaatgga gattactact ggtgttagct taaataatgt
     2521 gagaatagaa gaaataaaag aaataggtat taataataaa ggatttggtt ttaacctgat
     2581 tatgaatctt atggtcgacc aatgtggata ttgtggattt atgtttgaaa aaatgtgcta
     2641 tagccagata actgccagaa ttactagatg tggtcagtat tataaaagtg ttgattataa
     2701 tgcttatgag aatatggaag atagaaaaga aaaagcttat tccatttttt atggagatat
     2761 tttagaaagt tgcaatattg tattgatgaa tagcaatatt gataccttag aaagtgattc
     2821 acttaatgaa cataagatat atgtaatcaa ggcaaatgaa acaaacaatg taatgctaac
     2881 atgttatgct gaagcagata actttataca atgtgcaaag ggcaacttgc tgttagaaaa
     2941 tggtgtaaat acttataaat tttataatgg tgagatttgt tctgtaggag gtgttcaaat
     3001 ttctgatata gatgaaaaag atttaatcaa aataatcgag aacactctgt atatagatga
     3061 taaaggtaag tatttaatgg ttcctagatt aaaaaagaat actgtaatat attcagtact
     3121 agattctaca gaagaaataa ataaattaca tggtggaata tgggaacaga ttgatgtaaa
     3181 agtattagct ggggaatcca tgtactatta taaaaagata aaagattaaa ttatcaaatt
     3241 aggaggtagt gattaatgga agagtctata ataattaaag atataagtgg ctggattaat
     3301 gtccttgatt atggaatttg taatgatgaa acaaaagatg taagttttaa gttgcaagag
     3361 attatggatg ctgtaccaga agattctatt atatattttc caaaaggaaa atacctattt
     3421 gaaagtggtg ttaagattaa gaaacgatta actttatgtg gagattctta tattttggcg
     3481 aatgttccta aaaatactgc tggagtaaca caatttactt ttaaaggaaa gaaaaatcct
     3541 aaaagtgaaa atatagcaat tataacagca aaaagtgtaa gacactgtat taagagcatt
     3601 tgcttttatt cagatagttg tgatagagat atgacatcta atcatgaacc tccaacagat
     3661 ggaaaaccta gatttcatca taatgtaatt ataaattata gaaatatatc ggccattgta
     3721 tgtgaagata aacagggaga atcaggtcat tatgaaaatc tatcttttag tggtttttct
     3781 ggaattgcac ttgatatgcc taataattct acagcaaatg atattacaat ttttacatcc
     3841 ggacttggaa ttagtacagg agaaaattca actattgcta acagtaaggt ttggggctgt
     3901 gttgatggaa tgataatttc tactggaact tttataaatg gtatgagggt agaagaaata
     3961 aaaaaagttg gaattaaaaa tattggtaga ggtttaaatt ttattacaaa tatcacaatt
     4021 gaccaatgtg gatattgtgg agtttggttt gattctatat ccaatgttca gatatcagga
     4081 aatatcacaa ggtgtgggca gtactattat gatgtaagct atgataatta tttaatgttg
     4141 gataatagag ttaaagaagc atattcttta ttctatggta atttattgga atcaagtaat
     4201 atagttttat caaataccaa taaagataat tgggaagatt tcaaagaaga taagcataag
     4261 gtgtatcttg tagaagcact tgaaaccaag aatgtactat taacatgcaa tatagatgct
     4321 actgactgga ttgttaagtc agaaaaagga aatttaacgt acaataatat gcgacaaact
     4381 gttatatttt acgatggaaa actaagtagt atcaatggaa ttggtatatc tgatgaagat
     4441 tatggggata aagtcaagat aaaaaaagga aatatatata taaatagtaa ggatattaag
     4501 atgcataaac cagaggtagg tgacattatc tcaacaatta ttaaagataa aagtattctt
     4561 tctgctcatt atgggggaac atgggagaaa attggtgaaa aaatagagtt tggaataact
     4621 gtacactatt ataaattgat agagaaataa gtaaatacgt agtatttgaa tagatgtatt
     4681 ttattggaga gctaggacta tagttttaat gtaaatggtt tggtaatagc atggattgaa
     4741 taatataaaa ataatataaa aaattaattt gattatgtaa aattctatga agtagtaaaa
     4801 ctgtgacttt agtcatagga ggttcaaact aagtaaaagg gaaaggaata tataatatta
     4861 tggaaggaag aactgggatt aaagatggaa gatggattaa tgtagtagaa agaggtatta
     4921 aaaatgatgg ttcgtacaac gtaggtcctg ctttgcagga tattattgat gaaatagaaa
     4981 cagatttttg tgtcttatat tttccaaaag gtaaatacct atttacaagt ggtgttaaaa
     5041 ttaataaaga aattgtattg caaggtgatt ctaattcatc tacaggcact acacaattta
     5101 taactcgtgg agtagaaaat atgagtatta ttacattgac agggaagaaa caatgtatta
     5161 aaaatataaa tttctattct gatagttgtg agatacaggt caatgaagaa ccaccgacaa
     5221 aaggtaatcc taaatatcat tatgagatga ttatcaataa gaatgaaaag aatgttgaga
     5281 tgaacaatgt atcagcaatc ctgtatgata acagtaagga tatgattaaa ggtctaggtc
     5341 attatgaaaa tatttatata agtggttttt cgggtacagg aattaggata ccatattact
     5401 caattgtaaa tgatattaca gtttcatcct gtgggctggg tattgataca ggaatagaca
     5461 caattatttc taatagtagg atatctaagt gtcaaaatgg aatgaggatt agaactggaa
     5521 ctagtataaa taatgtaagg attgaaaaaa tacagaaagt tggtcttata agtagtcaag
     5581 ttaaggggta tgaggggtat ggaagttata aaattaataa tattacgatt gaccaatgtg
     5641 ggtattgtgg cttttcattt gattcaatga aagatagtta tttttctggt attataagaa
     5701 ggtgtggtca gtattattat aatacagatt atgacacata tttaagtatt aaagatagag
     5761 tagaagaagc atattcttta ttttatggaa attattttac agaatgtaat attgatttag
     5821 ttagtgataa taaggataac tgggatgatg gcttagaata taaacataaa gtttatgtat
     5881 ttaaaaccct tcaaatacca aaattaacat tgagatgcaa tatagatgcc tatgattata
     5941 tcgttaaatc ggtacatgga ggaggtaatt taatgttaaa aaataacttt aacaattata
     6001 tatttattga ttcatctagt ccagaagtta ttaatggaat taattttaat tatcaaaaga
     6061 atggaaacct gttaaggtta aataatggta aattaagtta taaaaatgga aaggaagaaa
     6121 cagctatatt cagacctaat tatggtctta ttgtagcatc aacagattca gaatgtgaac
     6181 aaatagaggc tacctatgga gaaaaatggg agaagcttgg ttctgaagta attggaaaag
     6241 aaacggtata ttattataaa catcatgaac cagaaaaaac aaaccaaata caataaaatt
     6301 tgtattatat aatgatataa agttttaata tatagtgttt acctttaatg taatattgat
     6361 atatttgtat agtattaaat ttgttaaaaa gtaaaattgt agggtgcttg aaaatagcac
     6421 cttttttagt ttcattataa aattagtttt gaaagtttag gaataatgtg tatttatttt
     6481 taaagaatta taaccttatt aaggatattt taaatgctag aaatgaatca gtaaatcata
     6541 ctggaattat cttaaatgtt ttgattaaat taagtattta gagctataat tatattaaaa
     6601 atatattaga acataattat attactatgg aggttaaata tgatagtaag tgtgagtaga
     6661 agaactgata tacctgcttt ctatagtaag tggtttttta acagacttaa agaaggtttt
     6721 gtttatgtaa ttaatccaat gaatccaaaa caggttagta agatagagtt aaatccacat
     6781 actgttgatt gttttgtatt ttggacaaag gatgtaactc caatgttata tgatttaaat
     6841 aaattaaaag actataaata ttattttcac tatactataa catcatatgg aaaagaagta
     6901 gagacatgta ttttagataa aagaaaggta atagattcat ttaaggaatt gtctagaaaa
     6961 ataggcaaag aaagagtaat tttaagatac gacccgattt tcttaagtga aaagtacaca
     7021 gtagattatc atatcaaagc atttgcgagt ttatgtaatc agcttgatgg atttacagaa
     7081 aaatgtataa taagttttat cgatttatac aagaaaacaa aatttaatac taaagcatta
     7141 catataaagc caatagaaat tacagaaata gaaacattat caaaagagat ttctaagatt
     7201 gctcataagc atgggataac acttgaaatg tgttctgaag aatataattt aagtaaattt
     7261 gggattggaa aaagtaagtg tattgatgat agattaatat caaaaattat aggatgtgaa
     7321 gtagaggtaa aaaaagatag tacacaaaga gatatttgtg gatgtgttaa aagtgtcgat
     7381 ataggacaat acaatacttg cagacattat tgtctgtatt gttatgctaa ctttaattat
     7441 gcaaaagtag aagaaaactg taggctttat aaagaaggta ataaattact catagatact
     7501 ttaagagatg atgcaaaaat aagcattaga gatatgaaga ctattaaagt agataagaat
     7561 aaacaaatta gtatatttga ttaaaataaa tatactaatt accttaatat attaagaata
     7621 tagatacttt tgataacaaa attaaactta atagatattt tctatataag aaagatggtc
     7681 ataatatgtc aatttgacca tctttttgat ataattaaaa cacaaataat ttagtaacaa
     7741 aggaggcaga acttgaattt attaaaaaaa tatcctatac ctattgcaag tttgatttta
     7801 tcaattttta ctttaggtaa cttattacaa tcttatagtg aatcattacg taatgtaatt
     7861 ggatttatag gatttatttt gtatgcaatt tatatagtca aaataatatt acttagaaag
     7921 ggtgttaaag agcaattaga gaacccttta atatcaagta catttccaac tataacaatg
     7981 gcaacaatga tatttgctac atatataaaa ccattatctt tagaattaag tattatgatt
     8041 tggagcatag gaattgtggg tcatcttgtg ttaatagtct tatttagtaa gaagttctta
     8101 gttaaattct caataaaaac ggtatttcca tcttggtata tagtatatgt tgggatagtt
     8161 gcagctagtg ttacggcacc agcatttaat caacttttga ttggcaaaat tgctttttgg
     8221 ataggatttg tatcttatat agttcttata ccagtaattt taaagagagt atggataatc
     8281 aaggaaatgc ctgaaccatc tttaccaagt atagctatat tagctgcacc agggtcacta
     8341 ttattgtctg gatatattaa ttctttttca gataaaaata atgtagtatt atacttgtta
     8401 ttcatattat caattgtatt ttatattata gttttgggat acttaccgaa attattaaga
     8461 ttgccttttt atccgagttt tgctgcattt acattcccta tggcaataag tgcaatgggt
     8521 atgaaactaa tgaatggtta tttaactaaa tcaggtcatt ttataagttg gatttcatat
     8581 gcttctaagt tagaagagat aattgctaca gtgattattt tatatgtttt agttttgttt
     8641 attatatatc ttagtaaatc tgaaactaca tctaaagtta aataagaatt attaaatatg
     8701 tgtatttaat aggaatattg atataaatat atgaagataa gataaaccat agaaacttaa
     8761 tttagcttaa ttttattgga ggaaaacaaa tgaaaacaaa agaagaatat ttaaaagaag
     8821 ttagaaacat atcagaagaa tactttagaa aaggagagtt tttttgctct gaagcagttc
     8881 tccagacaat aaatgatgct ttaggccaac ctttaagtcc agagataact aagttagcat
     8941 caggatttcc gataggactt ggaaaggcac aatgtctttg cggagcaata tctgggggag
     9001 agatggcttt aggtatagta tatggaagag ttcatggtga agctatgaat ccaaagatgt
     9061 ttgagcatgc taaaggtttg catgattata ttaaaaaaga gtatggtgca acatgttgta
     9121 gagctattac aaagaagtgg gatggtgata actttatgtc tcccgaaaga aaagagcact
     9181 gtattaaaat aacaggtcaa gttacagagt gggttgcaga taagcttata gaagatgaaa
     9241 aacttaatat caaatagtgc aatattagtt ggtgaattta taaagttaaa tttacttata
     9301 gtataagata cttgatattc tcgtattgat agaaaaattt atagcatatg tatttaatga
     9361 aatatactta tatttagagt gtggtttaat ttcataaata catatgctat tattttaagt
     9421 atataagcaa atttatttaa ataaatagaa acagttaatt tatatgtaca aaactatata
     9481 tgattagtgt aaataaatac ctattttata atactcaaca taaagacacc agacatgatt
     9541 agtattatac caataaaaat agcaagccat tcttttttgt tcttcttttc tttaaaaaat
     9601 ataatccctc ctaaagtggc tactatcaca caactctgag agatagaaaa acttattgta
     9661 actcccaatg tattagtcgt tatgaacata cctaaatttg ctattgacca caatatacca
     9721 gtaattaaat tgaataaaac attttccata tatacaatct ctttagaaaa atacttgaca
     9781 ataatagatg aagttatcaa cataccaaca gattgaggta atataattct atatccttca
     9841 attaaaaata tttgattagt tacaacatat aatgataaaa atagagatga taataataca
     9901 actccaacac attttaacat atttttatct gtagattttt tagtttgata atttgtgaga
     9961 agtataccaa gtattataca taatattgat aaagaaccaa tcagtaacat tttagaactg
    10021 gtccattcat taaacaagat tacagctaat aatgtcgtaa aagttaattg tgaaccattt
    10081 gaaattggca ttactttaga tacactagca atttgaattg ctttaaactg cattaattgt
    10141 cccagactcc agaatatgcc agaaatcata cttatacaaa atgaaaaaaa tgtaatttca
    10201 ggatgtataa ccagagttat tatagtggaa aatataaacg cacttatgga tgtacctaaa
    10261 agctgttcat gaggagaact tttctttaga tttgcaacta tgggcatcaa tccccatccc
    10321 attacaggaa gtaaagcaat aattattgtc agcattaaac tatttccttt catattaaat
    10381 ttagacagct tttaatttat catctaaaat taaagtgttc aatacataaa aattaccgta
    10441 tttaatcagg aaatattagt agttttttag aaaaacattt tccattaaaa taaagacaaa
    10501 gggaaggagt aaaattgaaa atgttaactg atatgattca aaatcgtgaa attaaaataa
    10561 taaagttttt attaaaagaa ggtcatacaa ctgtaaaagc aatagcagat gatttagatg
    10621 tttcagaaaa aacagtttca aaatctctta aagaagtaga agtagtgctt aaaaagatag
    10681 gtctttcttt aataagaaaa cctaagatag gtgtttatat tgatggagat ttaaaaaaaa
    10741 tacttcctta cctagataat aaaggaagtc aaattcctac aaccagagag gagagagtta
    10801 tctatatata ttcagtactt ttaaagagta atgattatat tactatacaa caactttctg
    10861 atgaactata tataagtcgt ggaactattg aaagggattt agcagaagtt gaaaaactgc
    10921 taaaaaacga agggatttcc atatataaga aacctagtaa gggaataaag ttaaatataa
    10981 gtgaaagaga taaaagacaa cttactgcta agtttataca taaattttgg agtagaaact
    11041 ggtatattaa gcaagagcaa gaaagctttt atcaagcatt tgaaaagata caggcagatg
    11101 taaatggtat tttttcggaa gatgatttat caataattat tgatattctt aggaatttca
    11161 gcagagaaag aaaatttgag ttctctgatt atgaatttca gtcattagtt attcatttgg
    11221 caatagctgt agagagaatt agaaaaggcg aatatataga agaaagtttg tatattggta
    11281 atatagaaaa taatcaaatg tcaaatacca attatttagc aaaacaatta gaagaaaaat
    11341 tttcaatatt attaccaaaa tctgaaattg gatatataaa tttacattta gtagcgtctg
    11401 accaacatag agatactaat gaactaaaaa atacttcaac taatccttgt gagacaagta
    11461 caaataatat atcaaatctt cgggaaatta taaaaaatac attgtatgag acagcttatg
    11521 acaaggattt aattgatgga ttaacttcac atgtacattc atcaataaat agactgaaat
    11581 atggacttag tattaaaaat ccatatgtaa atacaatcaa acaaaacttt tctttatcat
    11641 ttgagcaagc tttagattta aagaaagtaa ttgaatttat atatgatatt aggataaatg
    11701 atgatgaatg tgcttatata gcattacatt ttgaagcttt tagagaaaga atgaaagaac
    11761 ttccacaaaa tattagagta attctagtat gcagtacagg attaggttca tctaggttat
    11821 tgtctgcaag aataaaaaaa tactttccaa tgataaagat tgaaaaagta ctttccgtac
    11881 aagaattagt atccagtaaa attgatgcag atttagtaat aagtactatt tatttagagt
    11941 tagaaacaat tccaaccata gtagttagtc ctattttaaa tgaaagtgat ataagaacct
    12001 tggaaagaaa tatacaaaat gtttcaagtt gtacttctaa gaagtataaa tctttcagaa
    12061 acttaatatt tgaagaaaat atattgttga atattgaagc ttcaaattat gaagatgcaa
    12121 ttaagtatat aacagataac ctaataaaaa aaggttttgc aaaagaaggt atagctaaaa
    12181 gtgcaattga gagagaaaat ttatcttaca cgtctttcca gtcaatagct acacctcatg
    12241 ctaatcctag ttatattaaa aaatctaata tatctgttgc aacgttaaaa aatcctatta
    12301 catggggaaa tgaaaaggtg gaagttattt tctttatttc attagaaaat gacaaaaacc
    12361 tagagcttga tgaaatatat gaagtttttt atgacttcat tgacaataaa aaaaatataa
    12421 atgacatatt aagtgcaact tccacaaagg aagtgtacga tttgctagtg gaggagagta
    12481 tatagtgaaa atatcagatg tattgaaaaa agagcaaatt gtttttaatc ttgatacaaa
    12541 tacaaaggaa gaaacaataa aaaaattaag tagtatattt gttgaaaatg ggattgtgga
    12601 caatgaagaa gaatatgtta agtctgtttt ggaaagagaa gaacattcaa ctacaggtat
    12661 tggtaatggt attgctattc ctcatgggaa aagtaatgct gtcaaaaaat cttctataat
    12721 atttgcaaag actaagcata agattgaatg ggatgcttta gatgaaaaac cagtagatag
    12781 tattttctta ttggctataa gcaatattga taaggatagc aatcatcttg tactattatc
    12841 taaattagca actaaactta tggatgatga caatgtagat gcactaaaat tagccaattc
    12901 agaacaagaa ataatcaata tatttagtga agaaggagag atgtagaaat gaaaagaaaa
    12961 attatagcag ttacagcatg tgcaacagga gttgcacata catatatggc agcacaagca
    13021 ttaaagaaag cttcaaaaaa gatgggtcac cttataaaag ttgaaacaca aggagctaca
    13081 ggaatcgata atgagttaac tacaaaggat gcacaaattg cagaagtagt aatatttgca
    13141 gtagatacta aagtcagaaa tgctgaacgt tttgagggaa aagaaatact agaagtacca
    13201 gttaatgcac ctataaagga tgcagagaaa gtaataaatg atgcattaaa gttaattgac
    13261 actaaataaa tggagggaaa tgtaatgttg aaaaagatag tctctgagtt aaaaaagcat
    13321 gtcttaactg gaatctcata tatgattcca ttagtaattg caggtgctat gataatggcg
    13381 atatctcgtg taggtggttc tttctataat ataccagata tatgggatgc taaatatgca
    13441 gaaagtgcta gtagtattgt tagattatta catgatttag atgggtttgg aggtactgca
    13501 ttaggattaa tgtttcctgt aattgctgca tttataggat tttctatagc tgataaatta
    13561 gctattgtac caggtcttgt tggaggtatg attgctaaag atataggtgc aggattttta
    13621 ggagctttgg cagttggtct tatagcagga tatacatgtt tgtttattaa aaatcatgtt
    13681 aaattaccaa aatcagcagc ttcaatagta ccaatattta tagtaccagt atttgggaca
    13741 ttaattactg ttgtattaat caattatgtg ataggtatac cttttgccac attaaatact
    13801 ggattagaaa attggttaaa tggattatct ggtacaaatc agatactaat ggcagctatt
    13861 ataggggcta tgatggcagt tgacttaggt ggaccagtta ataaagcagc tttaacaaca
    13921 tcacttgcat tattaacaag tggaatttat gcgcctaaca ctgctgcaat ggtaggaata
    13981 gttataccac cactaggatt gggacttgct actatactag caaaacaaaa atataataaa
    14041 cagttacgtg aagctgggaa atcatctctt ataatgggtt taattggagt aagtgaagga
    14101 gcaattcctt ttgcagtaga atctccatta aaagtaatac cttcatgtgt aattggaaca
    14161 gcaattgctt cagctatggc agttggttta ggttcagtaa atgcaactcc aattagtgga
    14221 ttttatggat ggtttacagt agagaattgg ccaatgtatg tgttatcaat agcagttgga
    14281 acagtaattg tagctggatt agttatagta ttgcgtggaa atccagtgat tgaggatgat
    14341 gaatttgaag atgatgattt tgatgaagca gagtgggaat cttaaaattt aataactatt
    14401 taagacgtgt tataagctat attcaatatg aatcttatac taaataaagt gttaaatatt
    14461 cagtttagac ttaaatataa aagataaaga acagaggaga ataaatgata aaagcacata
    14521 ttgttaatca tacacactgg gatagagaat ggtattttac ttctggagat gcattagtac
    14581 ttagtgaaca actatttaca gatgtaattg atgaattaga acgtaatcca gatgtaagtt
    14641 ttgtacttga tggacaactt tctattttgg atgattatgt acaattacac aaagaaaaaa
    14701 tagaaatcat taaaaagtta ataaaagaag atagactaca tattggacca tggtttactc
    14761 aaactgatgc attctttgct agaggagagt ctattttaag aaacttaatg attggaattt
    14821 ttgaaagtaa aaagtatggt aagtatatga aaattgggta tttgccagat acatttggat
    14881 ttaatgcaca gatgcctata ctattaaagc atgtaggtct tgataatata ttcttttgga
    14941 gaggtataca tcttggtaaa caggttcaat caccatattt taagtggaaa agcttaaatg
    15001 gagaaagtta tatatatgca gtaaatatgc cacatggata tggaactggt atgcttttag
    15061 agccttcatt aaagtatgtt aatggacgct tagacccagc tattgatttt attaaatcat
    15121 atactgatgt agatgaggtg ttaatacctt ctgggaatga ccagttaaat ataataggtg
    15181 atttcaaaaa taaaattaaa gcgattaatg atatcggaaa gtattcatat ataacgagtt
    15241 catatcaaga gtttttaaaa tatataaagt ccatagaatc actagaaagc tatagaggtg
    15301 aatttagaga acctgtatta gcaagaatac ataaatcaat tggttctagt agaatggata
    15361 taaaattagc atgtgataaa ctagaaaaca agctgattaa aagaatagag ccattattag
    15421 taattgctaa aaagagtaaa attgaaataa gcaatcagtt acttataaat acatggaaaa
    15481 aacttttgga aggacaagca cacgatagtt tagctggatg tgtaactgat actgtaacag
    15541 atgatattct tcaccgtata agagaagcaa atgaaatatg tgacagtatt gaaaatacta
    15601 ttgtaaaaaa gatttcagaa gggcttaaat tatctaaaaa tgatatttta gtatttaata
    15661 cagaagctaa gcgttttaat ggatataaag agatacaagt tgtaagtgat agtaaaaata
    15721 tttattttaa agataattta gatgctacta taattgaaga aacttatgta aaaccgagag
    15781 aaaatgtatt agaagaaaca cctgctggca acgtatttat tgaagaacca ggatattata
    15841 tattaaaagt aagaataaat gtacagcttc ctgcacttgg ttataaagtt gtcagttttg
    15901 aatcaagtga taaagaaatg gtatcacttg ataaatctaa tgacactttt atatctaatc
    15961 ataattataa gataatctat gaagatggaa gcttaaattt acaacttaaa gatggaactt
    16021 acatatcaaa ctttttaata ttaaaagata gtggtaatgc aggtgatacc tatgactttt
    16081 ctccactaaa aaatgatgag gatataaaac tttcgtttga taaagtgtat acagaaaaag
    16141 ctttaggata tgaaaaaatg gtggttaaag gtactacttt actaccactt acattagaag
    16201 atagaaaaaa caaatatctt aatggtaaat taaatgtaaa agtaagtatt gtcttatcta
    16261 aagaaaaccc attgatggat gtaaaacttt gtgttgataa cacgatatat aatcatagac
    16321 tacgtgttca tataaaaact gatataaaag ataataaaaa catagcatca ttgccatttg
    16381 gatatataac aagagaaaat ggagttttag acaactggga agatatttac agtgaaatgc
    16441 ctgtagattt agaacctttg gaaagtaata ttacgcttac aaaccaaaat agaagttgta
    16501 ctgtatttac tagaggaata aaagagtatc aacatattga agatgaaatt gcattgacct
    16561 tacttgctac tacagatgaa ctaggaaagc ctgatttatt atatagacca ggacgtgctt
    16621 ctggtgatac aactaaaaaa ggtcatataa gaattaaaac agataaagct caaatgctta
    16681 agaaattaga attttcattt gcaatatata tggattctaa atcatttgat gaattggaaa
    16741 ttgcgaattt aacccacaat tatctaaggg aatgtgtaaa ttatcaatta caggattaca
    16801 atttcttttt atatcgtata gataataaga ttcaaaaatc aattgtcaat aaaactgtaa
    16861 gtagagaatt agaaataata tctttaccag aaaactatct tatatcagca tgttatcctt
    16921 cttattacag taaagataaa tttataataa gagtggaaaa tccaagtagt aatagaatga
    16981 tattggatga gcaaatattt aaaaatagaa atgggaaaat tgtaaatgca atagaagatg
    17041 tagaagaaga acaggtatat gaaatagcac cttttgacgt tatttcaata ttattggatt
    17101 tataagtgta aaagtaagtt tattgtttaa agtctgacag atattaatct gccagacttt
    17161 ttaatttgta taaatttggg taaatattaa tgaatggttt tagtatttaa atatgaattt
    17221 agtatttaat atagaattta ttgaagtaga ataattagtt taacaggata agcagtttaa
    17281 ata
//
LOCUS       DACXMZ010000070        17278 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.070, whole genome shotgun sequence.
ACCESSION   DACXMZ010000070 DACXMZ010000000
VERSION     DACXMZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.070"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            70..2139
                     /locus_tag="JD016_09060"
                     /pseudo
     CDS             70..2139
                     /locus_tag="JD016_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            2136..3422
                     /locus_tag="JD016_09065"
     CDS             2136..3422
                     /locus_tag="JD016_09065"
                     /inference="COORDINATES: protein motif:HMM:PF13245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAU4999786.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     DLLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDNNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDIHR"
     gene            3388..4002
                     /locus_tag="JD016_09070"
     CDS             3388..4002
                     /locus_tag="JD016_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999787.1"
                     /translation="MIGIIFTIFIGELYGIDEGYNPKILNDFITKVELMINDLKVNLN
                     ITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLVWIEYNETKDWI
                     KAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDEDRCAFFVALSR
                     AKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            4318..4941
                     /locus_tag="JD016_09075"
     CDS             4318..4941
                     /locus_tag="JD016_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999788.1"
                     /translation="MKMDNKIVFCVEPGIKTSSGGGYISEELGDKFISTTLPNYHKRK
                     AEILEKVNRHNILPSWNNEKVAVKSGETIELEDKNGIIREMQLESNNTGTDILLEENT
                     LKITANKNSNSGVISYIKIPKNEVGTSIVYCKHNYQSLSEFHMESSMSANLNINVIKL
                     GIVKVKKIDEGTGKELANAKLRFEYDGDSKEIITGSNEIASIEDIIG"
     gene            4966..5454
                     /locus_tag="JD016_09080"
     CDS             4966..5454
                     /locus_tag="JD016_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAU4999789.1"
                     /translation="MYEKDYKQYIKLDIPIDEAKIKNRFEIRLKNERAYHAAVDLLAN
                     RDEEKTAFSIINRYIRFVDKDDEKIRSEWKTNDQWSYFIGEGRAKLKLTTNPEPYNIQ
                     KTLNWLSRQVAPTLKTIKRLDSLNNSTLIEGLIRYARLTDKHKKIIEQNSVNVEDIIY
                     DK"
     gene            5507..5728
                     /locus_tag="JD016_09085"
                     /pseudo
     CDS             5507..5728
                     /locus_tag="JD016_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455630.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5968..6195
                     /locus_tag="JD016_09090"
     CDS             5968..6195
                     /locus_tag="JD016_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999790.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            6192..9131
                     /locus_tag="JD016_09095"
     CDS             6192..9131
                     /locus_tag="JD016_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAU4999791.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLIEEIYNQSIEDFSEESEFSYLKISDPLFWDQYTKQYTKTHLNDYLLDNITSLTN
                     KLKKKYKVKFATKLVLRLIFIRYLIDRGVDIAYKNFNHDIKASQKELLKITKNKVELY
                     ELFKYLKEKFNGNLFELNDEINNTCLTDEVFILLSDFLSGKISIDNGQMSLFSMYDFN
                     IIPVELISNIYEILLGKEIRNKDNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSC
                     GSGVFLVNSFRRIIDMNLGDKLFCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLT
                     ILDYKDPKILSEFKLPNLKGCNLIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGL
                     HLDYCNKNGYNDMQQNKEISRSFIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFRE
                     FLLKKSQIFSVVEMSSVRKLVFKNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFF
                     KLFNIIVIEKYDVKYISQKLLLENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILK
                     NSFVYGTGIQINGNESHDATHLKGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKAN
                     QQEIFIPPYVLLKKGVDTKTYRYRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGML
                     NSSFYSYLNIMLGTSIGIEREQGFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMF
                     LNEKNSCKYIKQLDKLILKSFNLNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLI
                     NYSECFKEQFSSIYRRVGKSISISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNN
                     MELFTKIIRYDYNDVFHQIRDIIHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQIL
                     SDTGGEE"
     gene            9131..9805
                     /locus_tag="JD016_09100"
     CDS             9131..9805
                     /locus_tag="JD016_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999792.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            9966..10382
                     /locus_tag="JD016_09105"
                     /pseudo
     CDS             9966..10382
                     /locus_tag="JD016_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            10711..12789
                     /locus_tag="JD016_09110"
                     /pseudo
     CDS             10711..12789
                     /locus_tag="JD016_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838041.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            12929..13351
                     /locus_tag="JD016_09115"
     CDS             12929..13351
                     /locus_tag="JD016_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999793.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            complement(13449..13595)
                     /locus_tag="JD016_09120"
     CDS             complement(13449..13595)
                     /locus_tag="JD016_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003560128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAU4999794.1"
                     /translation="MLLENGTNIKDIQHILGHAKTSTTMDTYLHVTNNMRNQTVNIFE
                     IIVE"
     gene            13889..14923
                     /locus_tag="JD016_09125"
     CDS             13889..14923
                     /locus_tag="JD016_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HAU4999795.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGITGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     TYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            15010..15213
                     /locus_tag="JD016_09130"
     CDS             15010..15213
                     /locus_tag="JD016_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HAU4999796.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            15384..16544
                     /locus_tag="JD016_09135"
     CDS             15384..16544
                     /locus_tag="JD016_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HAU4999797.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDAIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTNLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            16804..16944
                     /locus_tag="JD016_09140"
     CDS             16804..16944
                     /locus_tag="JD016_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999798.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
     gene            17123..17278
                     /locus_tag="JD016_09145"
                     /pseudo
     CDS             17123..17278
                     /locus_tag="JD016_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895106.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         7076 a         1678 c         2996 g         5528 t
ORIGIN      
        1 taataaagga aaaaatacag aattaaaaaa cataagatag ataattggac ttaggggaga
       61 gaaattatta tgtaattgac aaaaataatt cttagtaact ataggtgttt tggagaagaa
      121 cctacaacta ttgaaattaa taatttaact ggatttatag gtcataatag cagtggtaaa
      181 actgctttat taagtgcact tatgaaactt tttggagaaa agtctagtga tagaattatt
      241 gaaagatcag atttccatat acctgaaagt attgatccag atagtatttc taagaatgaa
      301 ttatatattg aagcacaatt tgagtttcct gaattggaga gaggtaaaga cagcatatct
      361 ataccagtat tttttaaaaa tatggttgtt agtgaaccag gtgggaaacc ttatttaagg
      421 attagacttg aagcaaattg ggagaaaagc tcaaatcctg aaggaagtat tgattcaaat
      481 gtatactata taacatctac aacagaggag ataaatcctg aagataagcg taatatcaaa
      541 agatatgagc ttgatcaaat aaagatgatt tatgtacctg ccattaggag accagatgaa
      601 cagttaaaaa atgtctcagg aacaataatt tcaagattat taaatggtat aagttggtca
      661 gaacaaacaa aggcaagaat taaaacgaaa gcaagtgaaa cggaggaaat tttctttgaa
      721 gaaaaagggg ttgaatcatt aaagaaatca ctcgaaactc aatggaatag ttatcataag
      781 gatattagat attctaatcc aaagataaac ttcaatagta ctgatttaga gacaatttta
      841 aaaaaagttg aagtaacttt ttcacctaca gggacacatc gagatcatcg ggttgaagag
      901 cttggagatg ggttacgttc tttattttat ctttcacttg taaatacatt tttagaaatt
      961 gaagaagaaa ttctaaaatc tattaatgat ggtaaagaag aagatatatt tacattaata
     1021 ccgcctgtat taacaattgt tgcggttgaa gaaccagaaa atcatatttc acctcatctt
     1081 ttaggaaggg tagttgaaaa attaaatttt atttctacaa aagaaaattc acagacaata
     1141 ttgacatcac attcaccagc aatagtaaaa agaattgaac caacagacat aaggtatttt
     1201 aggatttgta aggaatatga atgtacaaaa gtgaagtcaa ttatattacc aaatgaagaa
     1261 aaggaagcag aacaatttaa atttgtaaag gaatcagtat tagcatatcc tgaactttat
     1321 tttgcaaaac ttgttgtatt gggcgaaggt gatagtgaag agatcattat taagcagatg
     1381 attgaattaa aagcagaatc tgtagattca tcagaaattt caatagtacc attaggtggt
     1441 aggcatgtta atcatttttg gcgtttatta aatgatttag aaattccatt tattactttg
     1501 ttagatttag atagagaacg agatggaggt ggttggggac gtataaagta tgcaatacaa
     1561 caattaataa atattggtca tgataagaat gtattacttt atacatgtga aggaatttca
     1621 caagataatg aatttaaaga gatgcatact tgggatgtaa ctgatactga aaatatggct
     1681 aaatggattg aaatgctaaa aggttatgat gtttattttt cagctccgtt agatatagat
     1741 tttgcaatgt tacaaagttt taaagataaa tatatttcag cattaacaag tgcggaaggt
     1801 cctaggataa aagatcatgg aaaaatacaa gatattgtga tcaatgaaga tgctgaagat
     1861 gaatcattga aagctgctta taaagataga agagatagtg atattaaatc aaccttaaaa
     1921 caagaaggtg gcgatggatc tacttatact gagggagaga aagaactaat gatatggtat
     1981 tcatatttct ttttaggaag aggtaaacca actactcatt tatctttatt ttctaataat
     2041 actgatatag attttgaaag ttttccatac tgtctaaagg agtttgtaaa tagtattatt
     2101 gaaaagatga tagatgattc agggggtgga agaaaatgaa gaggaattgg gttagtaaag
     2161 aagattggag tccagcggct ggattcagac tagaagttga agcgatgaat acagttatta
     2221 atgataagaa tacacttgtt gtagctggtc ctggcgctgg aaaaacagat ttattagctc
     2281 agagagcctg ctttttatta gagactaata cctgtagata tcctaaaaaa atccttgcta
     2341 ttagttttaa aaaggatgct gctgaaaatt taaaagagcg tttagaattg agatgtggga
     2401 aagaattagc ttcaagattt gaatcaaaga cctatgatgc ttttgcaaaa gaaatcgtag
     2461 atagatttag aaatagttta gatgaagcat atagaccttt aaataattac aatatagctg
     2521 aaaatcgtga tgttagaaga gcatttgaga tagcaggatt aaatttcaga ggaaatcaaa
     2581 ctgaatttaa taaagtatat ggaaataaac tatcgataaa taaattgcct ttacatacag
     2641 attttgtaga taagattttt atagatgctt ggaatgtact acttaaaggc aacaaagata
     2701 ataattttgt aagttcactt acttttggta tgatatcacg tttagccgaa tatttgataa
     2761 gaaccaatag gtatattaag aaagcaattg aaatgactta tagtcatgtg tttttagatg
     2821 agtttcaaga tactaaatct attcaatatg atcttgttaa agcttgtttt tttaaaacag
     2881 aaacaataat tactgctgta ggggatggca aacaacgaat tatgttatgg gctggtgcta
     2941 gaaagtcaat atttgaagat tttcaaaatg attttttaag tcaaaaaaga acattaatta
     3001 tgaatcatcg ttcagcgcca agacttgtag agattcaaaa agcaatgtat aacagtttga
     3061 atgaagaggc aattgattta aaaactaatg aaaagtggaa ttatgaagat ggagaagtgt
     3121 atttaagact ttttgaggat tatataaaag aagctgatat acttagtgag gaaatagtcg
     3181 aattggtgga aacaggtata gaaattaatg aaatatgtat attagtaaaa cagagcgtag
     3241 atatttattc aaaagagatt ataaataaac tttctgaacg taatattaag gcgcgtaatg
     3301 aagcaatgta tcaggagata ttaaaagaag atataataaa gacactggtt tcgcttttta
     3361 ggttgtcgtt aagtgataga aatcccgatg attgggatta tatttacgat attcataggt
     3421 gaattatatg gaatagacga agggtataac cccaaaatat taaatgattt cattaccaaa
     3481 gtagaattaa tgattaatga tctcaaggta aatttaaata ttactgatga aaaacaattt
     3541 tcagatttag ttgattctaa ggttgaatat atttcatatg aaaaatttgc atcaaagttt
     3601 caaaaatata aatctaaaga ttattttgat gatttggtag ataaatttaa aaagctggta
     3661 tggattgaat ataatgaaac taaagattgg attaaagcga ttgataactt tgaaggtaaa
     3721 aattcaattc caataatgac aattcataaa agtaaaggac ttgaatatga tactatcttt
     3781 tttgttgggt tagaagattc agcattttgg aattttaaaa atcaaccaga tgaagataga
     3841 tgtgcatttt ttgttgcgtt atcaagagct aaaagaagaa ttgattttac atttagctta
     3901 gatagaccag caggattttc gaattcacag aagcattatt gtattaatga attctacgaa
     3961 ttgtttgata atactgagat tgtaaaaaag atagagtatt gattaagatg ataaaaactt
     4021 tagtatatta tattttaaga tattagttat ataagtagac attgaattta tgtatttgat
     4081 gtctattttt tatataaaaa tatattaaag aaaggatgaa tttgcatgaa gaaaaaaaca
     4141 aaaatatcta aaaagattgt aagttctatt atggttttta taactatagt tactactata
     4201 cttacatcat cagtttcagc ttatgctagc gagttggata tgagtgaaca gacaggcttt
     4261 aattatttag gtattagtcc aattacagga agaaaaatta atcataacat ttatcgtatg
     4321 aagatggata ataaaattgt attttgtgta gagccaggaa ttaaaacttc aagtggagga
     4381 ggatatatat cggaggagtt aggagataaa tttatatcta caacacttcc aaattatcat
     4441 aaaagaaaag ctgaaatact tgagaaggta aatagacata atatattacc ttcatggaat
     4501 aatgaaaaag ttgcagttaa aagtggagaa acaattgagt tagaagataa gaatggtatt
     4561 attagagaaa tgcaattaga atcaaataat actggaacag atatattact tgaagaaaat
     4621 actttgaaaa ttacagcaaa taaaaattct aattcaggag ttattagtta tataaagatt
     4681 cctaaaaatg aggttggaac atcaatagtt tattgtaagc ataactatca aagcttatca
     4741 gaatttcata tggaaagttc gatgagtgca aatttaaata taaatgtaat caagttagga
     4801 attgttaaag taaaaaagat tgatgaaggc acaggaaagg aattagcaaa tgcaaaactt
     4861 agatttgagt atgatggtga tagtaaagaa attataacgg gttctaatga aattgctagt
     4921 atagaagata ttattggttg attaaaaagt gaagtatact tttgtatcta tgaaaaagac
     4981 tataagcaat atataaaatt agatatacct attgatgaag caaaaataaa aaatagattt
     5041 gaaataaggt taaaaaatga aagagcatat catgcagcag ttgatttgtt agcaaataga
     5101 gatgaagaaa agacagcatt ttcaattatt aatcgttata tacgttttgt tgataaagat
     5161 gatgaaaaaa ttagaagtga atggaaaact aatgatcagt ggagttattt tattggtgaa
     5221 ggtagagcga agttaaaact aacaacaaat ccagaaccat ataatattca aaaaacttta
     5281 aattggttat caagacaagt tgcaccaacg ttaaaaacaa ttaaaaggtt agatagtttg
     5341 aacaactcaa cattaattga gggcttaata aggtatgcaa gactaacaga taaacataaa
     5401 aaaataattg aacaaaattc agtaaatgtt gaagacatta tttatgataa ataaaaaaat
     5461 gtaattagta ttgcagattt taatataaaa gaagggagat atacgaatga attttggtca
     5521 aaatttatat aattagtttt tatcaaatgc acaatcattg gtattaatgg ctatagctat
     5581 aattggtgta tatttaggat ttaagagaga gttttcaaaa cttataggat ttttagtagt
     5641 tgcattaatt gcagtagggc ttgtgtttaa tacagcagga gttaaagata tattattgga
     5701 gctatttaat aaaataatag gtgtgtaagg attaaaagag aatagaaaat tatgagtata
     5761 tgatgaaggg ttctaataag ttttattaaa atatatattg aaagacaagt agagctattt
     5821 cgagttatta catgtaatgc taatatatat aactgcattt tggaaattaa atacataatt
     5881 agaaatagca aattttatag gtgacttatt aatatgttta cttattgtat actatttgat
     5941 atatttaata tgaggtgatt cttgaaaatg gacatagtga aagtttttgc taacaatgtg
     6001 agaaaatata gaaataaaaa aggtctatca caagaagctt ttgctgaaaa agctggattg
     6061 catagaactt atatttgtgc tattgaaaga aaaaagagaa gtattgcttt agataatgtg
     6121 cagaaaattg ctgatgcgct tgagattgag acttacttac tatttataga aggtgaggag
     6181 aaagaatgtt tatgaaaaaa ttaccaatag aaaagcttat agaaaattta ggtttttcag
     6241 ggtcaaagaa tcttgtgaat atagatatgc ttacaaaatt agatatatct aaacatgatg
     6301 taaaaacttt aaaagttata aatccttatg caatttattt agtaaatagt aagccattta
     6361 tattatttat agagtcttct tttgaagaaa aaaaattaca acaaattagt aagcttatat
     6421 ggaatgctca aataccaatt gcttttatat gcgattctaa ttctgttaaa atatttaatg
     6481 gaaaagcttt ggatttaaag tcatatttaa ttgaggaaat atataatcaa tctattgagg
     6541 atttttcgga agaatctgag ttttcttatt tgaaaatatc tgatccatta ttttgggatc
     6601 aatacactaa acaatatacc aaaacgcatt taaatgatta tttgctagat aatattactt
     6661 cattgacaaa taaattaaag aaaaaatata aggtgaaatt tgcaacgaag cttgttctaa
     6721 gattaatatt tattagatat ttaatcgata gaggtgtcga tatagcttat aaaaatttta
     6781 atcatgatat taaagcttca caaaaagaac tgctaaagat tactaaaaac aaagttgaat
     6841 tatatgaact gtttaaatac ttaaaagaaa agtttaatgg taatttattt gagttaaatg
     6901 acgaaattaa taatacttgt ttaacagatg aagtgtttat tttactatct gattttttat
     6961 caggtaaaat aagtattgac aatggacaaa tgtctttatt ttcaatgtat gactttaata
     7021 tcataccagt tgaactaatt agtaatatat acgaaattct tttaggaaaa gaaattcgta
     7081 ataaagataa cgcattctat actccgaatt acttagcaga atatatttta gataaaacaa
     7141 cacttgaatt tctgaaaaat aataggcaat acaaaattct agatccttct tgtggatcag
     7201 gagtattttt agtaaatagt tttagacgta ttattgatat gaatttgggt gataaacttt
     7261 tttgtgaaga tgataaactt ttaaaagaac ttttattatc taatatttat ggaattgata
     7321 tcaatgaaga tgctatcgat gttactattt tctcgttata tctaactatt ttagattaca
     7381 aagaccctaa gatattatca gaatttaaac tgcctaattt aaaagggtgt aatttaatag
     7441 cgagtgattt ttttgatgat aataaattgg agagtttaaa gaaaattaac tttgatttta
     7501 tcattggaaa tccgccttgg ggaagtgtct cagatggatt acatttagat tattgcaata
     7561 aaaatggcta taatgatatg caacaaaaca aagaaatcag tagaagtttt atttttagag
     7621 caaaagattt tagctctaga aatacagtat gttcttttgt agtgcattct aaaatactat
     7681 atactcaaaa aaagccatca aaaaaattcc gtgaattttt gcttaaaaaa agtcaaatat
     7741 ttagtgtagt tgaaatgtca tctgtaagaa aattagtgtt taaaaatgca gacgctcctg
     7801 cagcagttat atcatttaag tataacgaaa gtgaagaaaa tttaaataat agaatcatct
     7861 atacatcttt aaaaccaaat gtatttttta aattatttaa cattattgta attgaaaaat
     7921 atgatgttaa atatatttca caaaaactat tattggagaa tgattgggct tggaaaacta
     7981 ttgtgtatgg attcagtaaa gatatagata taattatgaa tatcaaaaaa aaatatcaaa
     8041 ctgtaaatga aattattttg aaaaacagtt ttgtttatgg aacagggata caaatcaatg
     8101 gtaatgaaag ccatgatgcg acacatttaa aaggaaagtg gtatgttgat tcaaagaaag
     8161 gaatagattc attctatgtt gatataaata aagggaaaga acttactcaa gataaaatga
     8221 gaagacctaa ggctaatcag caagaaatat ttattcctcc atatgtatta ttaaaaaagg
     8281 gagttgacac gaaaacatat agatatagag cagcattttc aaatgatgca tttgtatatt
     8341 cagatgcagt tacaggaata tgtggaaaaa aagataattt aactacactt tattgcttga
     8401 ctggtatgct taactcttct ttttactcat acttaaatat aatgctaggt acatcaattg
     8461 gaatagaaag agaacaagga ttttcaacgg aaatttttac gtatccagca atagaaagtg
     8521 atgaaattgc taatctggta gtaaaaatac aagaagcgac taaattagaa aaaacaacta
     8581 tgttcttgaa tgaaaaaaat agttgtaagt atatcaaaca attagataag ttaattttga
     8641 agtcttttaa tttaaatgat gataaattta ttgactatgc aataaatatt caaattcctg
     8701 agcttacaaa ttctaagtca actaacatat atagaaaaat aactaaggaa gatttaatta
     8761 attatagtga atgtttcaaa gaacagtttt catcgatata tcgtagggtt gggaaaagta
     8821 tttcaatttc ggtatatcca aaaatattaa atcgttttac agtatttgaa ctagaagtaa
     8881 cagatgaaaa aacagataga gagattatgg ttaaagcaga agctgataat aatatggagt
     8941 tgtttacgaa aataattaga tatgattata atgatgtttt tcatcaaatt agagatatta
     9001 ttcattttgg agaaaattca ttttatatca taaagcctaa ttgctataaa tattggcatc
     9061 ctgctatcgc agaaatggat cttgctgatg ttttggatca gattttatct gatacagggg
     9121 gtgaagaata atgggaggat ttaaaaattc ttcactaaac ttttatttaa agcaagggct
     9181 ttatgaaata attatagatg gattaataga ctgttgttta cttatgagca aatcatgtat
     9241 tgaacaatct agaaaaatat taaatcacga agaaaagata agaacgcatt tagttgaaaa
     9301 ttatttagaa aatgatattg aaagaagaaa aacatcttta tctaaacaac atctaagatt
     9361 tgaagtagaa gcacaagaaa attatgacaa caaaaatgaa tcttatgagg gtagggttga
     9421 tataaaagtt atatctaaag acactttagc ggatagaagg gcttattata tcattgaatg
     9481 taaaaggata gatggtaagg aaactttaaa taaaaaatat ataaccgaag gtgtacaacg
     9541 atttattact gagaaatatt cttcgcatta tggcaaaaat ataatgcttg gatttatagt
     9601 aaaagatata aatatccaga gcaatataca aaaaattaat tcactgaatc agaacatatt
     9661 atatacatat atagatacag atatgttgac agttagaagt gattctgaaa ataattatgc
     9721 tgtttgtgaa agtaaatata aagtaaaatc taaactacta ttgttaaatc atatttttta
     9781 taacttttcg agtataataa gataatctta tgaatataaa atattactat gttaaaatta
     9841 tagtttggtt ttgataatat tataaaaata agtttaagaa taaaatataa aggaaaaaag
     9901 gggcgtagca ctatttttta gtgtgacagc ccctttaatt ttaaaatcgg taaggaggag
     9961 tgtatttgaa aaaaattaag tcatatacag ggatttggaa tgtggagaag gttatatatg
    10021 caataaatga ttttcagctt ccatttccag ttacttttac gcagatgaca tggtttgtat
    10081 taacactttt tataattata ttatttggta agttacctcc attatttatg atagaaggag
    10141 catttttaaa gtatcttggc ataccaattt ttataactta gtttatgtct caaaagactt
    10201 ttgatggaaa gaaaccatat ggatttttaa agtcaataat actatattca ttaagaccta
    10261 aaataacata cgcaggaaaa aacattaaat ttaaaaataa aattattgat gaaaatataa
    10321 ctatagtaag gagtgaatta tatgttcccg ataaaatata tagagaacaa tcttgtattt
    10381 aataaggaag gagaatgttt tgcatattat gaattaatac catataatta ttcattctta
    10441 taagctgaac aaaaatttat tgtacatgat aattttaggc aattaatatc acaaagtaga
    10501 aatggtaaaa ttcatgcact tcaaatagcc actgaaagtt ctataagagt tacacaagaa
    10561 aggttaaaga aaaatattac aggaaaatta aaagaaattg cttatgaaaa aatagacgag
    10621 caaacagagg cacttgtttc tatgattggc gataatcaaa ttgattatca tttttttatt
    10681 gggtttaagc ttttagttac agagaaagaa atgaatataa agagtataaa aaagtcaata
    10741 ttagctaatc tatcagattt tatatatgat gttaataata aattgatgga tgactttgtc
    10801 agtataaata atgatgaaat tatgagattt ttaaaaatgg aaaagttatt agaaaataaa
    10861 atctcaagaa gatttaaagt tagaaggtta gataaaaatg attttggata tttaattgaa
    10921 cataattatg gaaagaaagg cacagttttt gaagattatc aataccattt acctacaaag
    10981 aaacttaaaa gagaaacatt agtaaagaaa tacaatctta taaagccaac tagatgtctt
    11041 atagaagaga agcaaagata cttaaaaatt atacatgagg attcagaaag ttttgtagct
    11101 tattttacta ttgaaattat tattggagaa atggattttc catctagtga gatattctat
    11161 tatcagcaac aacagtttgc atttcctatt gatactagta tgaatgtgga aattgtaaca
    11221 aataaaaagg ctttaactac agttagaaat aagaaaaaag aattaaaaga tttagacaat
    11281 catgcttggc agtcagggaa tgaaacagta acaaatgtag tagaagcatt agaggctgta
    11341 aatgagcttg taaataattt aaatcaaaca aaagaatcaa tgtataagtt aagctatgta
    11401 attagggtat cagcagatag cattgatgaa ttaaaacgcc gttgtgatga agttaaagat
    11461 ttttatgacg atttaaatat aaaattagta cgcctaatgg gagatatgat aggatttcac
    11521 aatgaattta taccttcaag caaaaggtat ataaatgatt atattcaata tgtaactagt
    11581 gattttctag caggattagg gtttggagca actcaatagc ttggagagaa cgaaggaata
    11641 tatattggat atagtttaga tacaggaaga aatgtctatc ttcaaccatc tcttgcaagt
    11701 caaggagtaa aagaatctgt aactaatgcg ttggtagcag cattctttgg atttttagga
    11761 ggtgggaaat catttgctaa taacttactt gtttactatt cagtattgtt tggagggcaa
    11821 gctgttttgg tggatccaaa agctgaacgt ggcaaatgga aagaaaattt accagatata
    11881 tatcatgaag taaatattgt taatttaaca agtgatatag aaaataaggg aatgctagat
    11941 ccttatgtaa ttatgaaaga agtaaaagat agtggaagtc tagctattga tatattaact
    12001 tttcttacag gaataagtag tcatgatgga gagcagtttc ctatacttag aaaagcaatc
    12061 ataaatgtta ctcaagacaa atgcagagga cttttgaagg taatagatga attaaggaaa
    12121 gataattctc aaatatcaaa taatattgca gatcatattg aaagttttac agattatgat
    12181 tttgcacaat tacttttctc agatggaaat attaaaggct caattaatct agaaaaacaa
    12241 atgaatataa tacaagttgc agatcttgtt ttaccgaata aagatacttt atttgaagaa
    12301 tatacaacaa tggaaagtct ttcagtagct atgttgatag taattagtac ttttgcatta
    12361 gattttatcc atacagatag aagtactttt aaaatagtag atttagatga agcttggaca
    12421 tttcttaatg tagcacaagg aaaaacttta tctaataaat tagttagagc aggatgtgct
    12481 atgaatgcag gagtgtattt tgtaactcaa aatgcaggtg atgtatcaga tgaaaattta
    12541 aaaaacaata ttggattaaa atttgcattc agaagcacag atatcaatga aattaagaga
    12601 atacttgaat tttttggagt tgataaggaa gatgagaata atcaaaaaag gttaagagag
    12661 ttagagaatg gacagtgttt aatacaagat ttatatggac gagttagggt aatacaaata
    12721 catctagttt ttcaagaact ccttactgca tttggtacaa gaccacctat aagaaaagag
    12781 gtggattaat gaaatcgaaa gtatttagaa atttaagctg gataactatt ttaatagtag
    12841 tacttttact attttttatt tatataaatt tatagattta gagaaacaaa tagatacatt
    12901 agataagaaa agaaaaaaac tcatagatat gagattagaa gatataattg ataaagaatc
    12961 atatgaagaa aagtatgatg atttaacagt tgaaataaag aaacttagta aagagaaaaa
    13021 aactcttaaa gcatcattaa taaatgaaaa gagtataaaa gcaagactta gagaatttag
    13081 aaagatactt gaaagaaatg aattgttaga aaaatttgat agatatgtat ttgcaagtat
    13141 agtagataag gttatagttg gagttattaa tgaagatggt atagttgatc cttataaaat
    13201 aacttttgtt tataaaacta gctttgatga taaacaagat ggacataaat ttataaataa
    13261 gagtccgtta gaaacgtatt caaaagataa caacgataat aaaaagaagt gttcatatac
    13321 taataatgag acattgcgag actgtagtta aacttattag agaataacaa ggtttcagac
    13381 ttttatattg tgtcttaaaa gtagatttgc cacctatttg ccaccataat tatttttggg
    13441 tggcaaattt attctacaat gatttcaaaa atatttaccg tctggtttct catgttatta
    13501 gttacatgta aatatgtatc catagttgtt gaagttttag catgtccgag tatatgctgg
    13561 atatctttta tattagtacc attttctaat aacatagtat cagggttttt aaaataaagt
    13621 gtgtattctt ctaaagtagt gataattaaa atatcaaata tcactataca aaggaaagtg
    13681 cacgcttttt atgtttaaca aaaacgaaat attaaaaaac ttattgaaga atatgatgtc
    13741 aaaacaacta cagatattca agatatgcta aaagatttat ttacaaatac aattcatcaa
    13801 atatttacat tgtcgaattt taacggttta aacataatat attgactgat ataataaaat
    13861 catagaatat tttaggggga aatagattat gtttaagaaa aagttaatag ctgttgcaat
    13921 gagtataact atgattagtg tagggagctt ttcgtatgct cattcaggaa gaactgattc
    13981 atcaggaggt catcgtgata ataaaaataa atctggatta ggaagttatc actatcattg
    14041 tggtggttat ccagctcatt tacataataa tggtggttgt ccttatactg gaggaggaag
    14101 cagttctgga acaacaactt cggttaataa tgaagaaaag caaaagaggt ctgttggaga
    14161 aaaaggatat aatcaagggt atgaagatgg gtataaagga aattatagtt catcaaatta
    14221 tagtggagat tatagtgata catatgaatc aaagtactca gaaggatatg aaaaaggtaa
    14281 ggctaaatta gaagaggaag aaaaggtggc taaagagact ggatataatt tagggattac
    14341 aggtgctaaa tctaataaca catatgaaaa agaagtttta aaaaatgctt atgataccgg
    14401 atattcaact ggatacaatg aatataagac taaaaagata gaagaatata aagctaaagg
    14461 gatagaggat agcaataaag ataaagaaaa aatgacattt gaagaaaaca tagattctga
    14521 atttatagat gcttataata atacctatga cgaaatacaa gaacaattaa aaaatgatta
    14581 cactactcaa ggatttgaga gtgctatcaa aggagaaaga tttgacacat caactatagg
    14641 taatgttaaa tatgctaatt ggtttaaaga aggatatgat aatggtaagg ttaagttacc
    14701 taaagcaaaa gaaagtgtat ataatcaagg ttacaatgaa gaagatttta gtataccaga
    14761 tgaaatgaag tctattgaaa ctaagttaaa aggtgtatat gatgaaggtt tagaaaaaag
    14821 agaagaagag aaaagtagaa atgctactta tggggttgga gctggaacag cagctgtagt
    14881 tgctggagta gtatataaga agaaaaaacg taaaaagata tagatgctag atataccaat
    14941 gatgacaagt gtataacaaa tataatttta agtataaata tttaaaatat agaagggaag
    15001 gttatatcta tgggatttaa ctttaggaaa agcataaatc taggaggagg actcaaatta
    15061 aatttaggta agaagagtgt aggtataagt gctggtgtta aaggtgctag agtgtcagtt
    15121 aattcaaaag gaagaaaatc agctacattg agtatacctg gaactggaat atcatataca
    15181 aaaactagta caaagaaacg taaacctaaa taaatatttg atatactaag aaatagactg
    15241 ttacaaatag tctatttttt agtatataga tttaaaaagt gacaaaattt tgaaatataa
    15301 actaatttca taaaggaaaa tattctttta tgtagaatta taatatttaa gtaatattat
    15361 aaaatttata ggaggatttc attatgaaat taagcaaaac actattggca atagggatgt
    15421 ctgtttcatt attgattgct agttgtccaa catcaaacgc tcttagttct attaattcaa
    15481 taaaaggtac aaataagtat gaaacagcag gtttaatagc agataaacaa aattatgata
    15541 ttgcaatact tattaattct gaaaatggat tatctgatgg attgtcagca agtggattgt
    15601 caggagctaa aaatgcccca atattgttaa ctaagaaaga tgcaatccct tcagaaacat
    15661 taaaaagatt agaaaaggta aaagaagtat atctaattgg aggaaaaaat tcgataggag
    15721 catcagttca gaaaatatta agtgataaga aaataagcat aaatagaata agtggaactg
    15781 atagaataga tacaagtcat aaagttgcaa aagaaatagg aagagtaagt acggttaaaa
    15841 aagtaatatt aactaatgct tttaaaggcg aaccagatgc tatgagtgtt gcttctatag
    15901 cagttagaga taaatctcca atagtattaa ctgatggaaa tactacaagt tttgctacag
    15961 atggattaga aacttatgca attggtggaa aagtcagtat gagtgataag ttaataaatg
    16021 aaactaaagc aacaagaata ggtggagtag atagatacga tacaaataaa caaataataa
    16081 ataaatttta taatggctca aaagaatttt acatagcaag tgggactaat ttagtttatt
    16141 ctttagttgg ttctacaatt gcacaaaaaa cacctatagt gttagttgga aatggaagta
    16201 ataagagtat attaaaaaat gcaagtaaaa taactgctat tggaaatatt gatgatgttg
    16261 ttataaaaca atgtttaaat ataactaata atgttggaga ttcaaataca ggtattgtaa
    16321 aagatactaa ttcaaataat acgaacaaac caagtaatga gcctagctct aataatcctg
    16381 gattaactga taaaagtaca gtatatgtaa ctcctaatgg aaaatcatat catctaacaa
    16441 aaaattgttc tacactaaaa agaagtaaaa caataaataa agtaacatta aaacaagcta
    16501 agtcacaagg taaaatagac ccttgtaatg tttgtgtaaa ataatgtatt agccaaatat
    16561 atattaaaaa agacaattaa ttaagattgt cttttttatt gaagaaatat aaatttatat
    16621 tatgtattat taaaaagaaa attattaatt ataaaaaata ataataaaga cgataagtat
    16681 taaaatttta tgtttccaat atttatgata taattaatat ggcaaggaat aaatatttga
    16741 aaaagctatg agtggtgttt ccatggaagt ttcttcactt tcttatgaga ggaggtgaaa
    16801 actatggaaa atctaatgat gagtattata actggtgtta tagccagtta catttacgat
    16861 aaattaaaga gccactctga gcgaccaact aagagtggct gggaacttaa tataaagttc
    16921 cagaaaagta aatcttcaaa ataatttgtt gaaggaaaca tcactcaaag ttaagtaaaa
    16981 tatgtttcct tgcttttatt atatcacaaa ttaaaaatat taacacaaaa ttttgtgaat
    17041 atatacttat tattgttcta aaaaaatatg taaataattg atttgattgc atggaattat
    17101 tgatgtaata tttagttaaa tatatactat ctcaattaaa gaagataagt actataaatt
    17161 catatcaaaa aatacataaa cttttaagtt ataattttat aaaaagtctt ttagtatcat
    17221 tatttcaaga ggaattatca tatgaaaaaa taacttcatt agaaaagtgg ataaaaaa
//
LOCUS       DACXMZ010000071        17197 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.071, whole genome shotgun sequence.
ACCESSION   DACXMZ010000071 DACXMZ010000000
VERSION     DACXMZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.071"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            78..623
                     /locus_tag="JD016_09150"
     CDS             78..623
                     /locus_tag="JD016_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIC"
                     /protein_id="HAU4999799.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            636..1649
                     /locus_tag="JD016_09155"
     CDS             636..1649
                     /locus_tag="JD016_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAU4999800.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSILMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1871..3844
                     /locus_tag="JD016_09160"
     CDS             1871..3844
                     /locus_tag="JD016_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAU4999801.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            3913..4272
                     /locus_tag="JD016_09165"
     CDS             3913..4272
                     /locus_tag="JD016_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999802.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4307..4663
                     /locus_tag="JD016_09170"
     CDS             4307..4663
                     /locus_tag="JD016_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HAU4999803.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4700..5992
                     /locus_tag="JD016_09175"
     CDS             4700..5992
                     /locus_tag="JD016_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAU4999804.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            6428..7066
                     /locus_tag="JD016_09180"
     CDS             6428..7066
                     /locus_tag="JD016_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999805.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            7073..7915
                     /locus_tag="JD016_09185"
     CDS             7073..7915
                     /locus_tag="JD016_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAU4999806.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            7976..10603
                     /locus_tag="JD016_09190"
     CDS             7976..10603
                     /locus_tag="JD016_09190"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HAU4999807.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            10793..11062
                     /locus_tag="JD016_09195"
     CDS             10793..11062
                     /locus_tag="JD016_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999808.1"
                     /translation="MSLLEGIKLTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCP
                     EPSCRALVSRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(11351..12661)
                     /locus_tag="JD016_09200"
     CDS             complement(11351..12661)
                     /locus_tag="JD016_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAU4999809.1"
                     /translation="MNKKMSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSD
                     SNKRTSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFP
                     YDQIGLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMK
                     LKKDLGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEY
                     RFESFRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVA
                     PTKPEQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFN
                     TENGLVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGL
                     ITLTDYKKQVVSNEDAKNSYDSLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(12683..15817)
                     /locus_tag="JD016_09205"
     CDS             complement(12683..15817)
                     /locus_tag="JD016_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAU4999810.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            complement(15821..17044)
                     /locus_tag="JD016_09210"
     CDS             complement(15821..17044)
                     /locus_tag="JD016_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAU4999811.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
BASE COUNT         6240 a         2203 c         2940 g         5814 t
ORIGIN      
        1 aaaagatgta atttgtaaaa tttatggtaa gcagtaaaat taaaaattta aagatattat
       61 agggaggtta taattttatg gaaaacgtta ttacaggtct aagtaaaggt gcagagtggt
      121 ttatcggttt gtttcagaaa gggggagaac aatttatagg tcttgtttca ggtactcttc
      181 caacgctaat tgtattaatg gtagctatta actctttgat taagataatt ggtgaggaaa
      241 gagttaataa ttgggcttct aagctaggaa agaactcttt tacaagatat attctacttc
      301 cattagtatc agtatttttc ttgggaaatc ctatgtgcta tacatttggt agatttttaa
      361 aagaagagca caaagcagga ttttatgatg cagcagtttc atttgtacat ccaattacag
      421 gtctttttcc acatgcaaat gcaggagaac tttttgtatg gttgggaata tctgcaggtt
      481 tgactacttt aggtaaagaa actactacat tggcattgtg gtattttata gttggtctaa
      541 ttgtaatttt tataagaggt atagtaacag aaaaaatgta tgcttttcta acaagaaaaa
      601 atagagcaac aaataaggct taggaggtag ataaaatgga ggagaataaa atattaaaaa
      661 tagaaaaggg aacttcaggc tggggaggtc ctttgtatat aaaaaaagaa gggaatagaa
      721 ataagatact atcaatgact gctggtggta tccatgaagt aacattaaaa ataaaagaac
      781 tactgggttg tgaaattgta gatggattta aaactggagt ttctgatgaa gaagtagcag
      841 tagttattat tgattgtggt ggaactgcta gatgtggagt ttatccaaag aaaaaaatac
      901 ctacaataaa tgtaaatcca gttggaaaaa caggtccttt agcaaaattt ataacagaag
      961 aatattatgt atcagatgtt aatcctaact gtataagtgt tgtagatggg gaagatatgc
     1021 cacaaaaatc tcaagaaaat aaaagcgaaa ataagagttc tattagaaag cctgataatt
     1081 atgatgaggt taagtcaaaa gcacaaggtg aatatgctaa aaagaacata atactttcta
     1141 ttggtcaagg ggcagggcaa gtagtatcaa aattttatga tgcaggtcga gatactatcc
     1201 aaatggtaat gaataacgta ataccattta tggcatttgt aagtatactt atgggaatta
     1261 ttttagcatc tggactggga gactggattg caagagttat atctccactt gcaggtaata
     1321 ttggtggatt attgattata tcagtaattt gcacacttcc attcttatca ccaatactag
     1381 gacctggtgc agttatagct caagtagtag ggactcttgt aggtacacaa attggtcttg
     1441 gtgcaatacc agcatattta gcacttccag ctttatttgc aataaatggg caagctggat
     1501 gtgattttgt accagtagga ttaagtttgg gagaagcaga accagaaact gtagagtatg
     1561 gtgttccagc tttattttac tctcgtttaa taactggtcc aatagctgtt attattgcat
     1621 atggagttgc agtatttgca ttgagataat atttaatgtc aatataaggt ttggaattaa
     1681 ttaataaaat tgtaaatata aatttaagct agtcaagatt ttttgttaat gaaagactag
     1741 cttttatact attaattagc tgtaattatc gagttagatt aatactaaat tttcctttat
     1801 taaagtgaaa ttttatgtat taatttggtt tcaaatttgt actagaatga aatcaaggag
     1861 gagatacaaa atgagtgtaa ctgagaaaat tccttctatt gaccgcaaga tatttaagat
     1921 attgattatg tgtagtaaaa aagagtttgt gagtattaac tcaatagcaa atgaattgaa
     1981 tgtcacaact agaagtgtaa ggacgtatat aaaacaattg aataaagatt taggtaatga
     2041 tatagcagta atcaaatata taaagggtca aggctataaa ctagaaataa aagatgagca
     2101 gatactcaat aagattattg atatgaatag aaaaaatgta ttttcattaa attcaaaaga
     2161 agatagagta gagtttatac taaattactt gatagaacta gatggattta ttacacttga
     2221 tagtttagct gatgagatgt gtgtaggaag aactacttta gtaaatgatt tccagtatgt
     2281 agaaaaagtt ttggcttcat ataatttaaa cttgattaaa aaacagaata caggtatgaa
     2341 attaaatgga aatgaacttg atattagact gtttatatta aatcagttgt ataaaaactc
     2401 aagaaaagat tttagtaatt caaagtattt taaaggaatc aaaaaagaag aaatcataaa
     2461 tttagaagaa aaactattga ctttatttaa aaaaaataat ttttatgtta cagatgaaat
     2521 gttaagagaa gttattaact acatagtagt tttagtctat agagtaaaag aagaaaagaa
     2581 agtaaaagat tatgatgtaa agtttgattt acttaaatca tatgatgaat actttattgc
     2641 tagggaaata aaacatataa tatcaggaat gtttgaatgt actttaaatg atgaagaaat
     2701 tatttattta actataccgt tagttagtgg aaatgcacca gcatcagaat gtgctttaaa
     2761 tagtagtaga attagtaaaa acattgatga gttgatggaa aatatattta atcaaatata
     2821 tgtcgatatg ggtattttta taaatgagga tgaattaaga gtaggtctag gatatcatct
     2881 tagttttact ttaaacaggc tattatttaa tataaagtta aaaaatgttt tgttagaaga
     2941 gattaagcaa aattatatac ttccatttaa attagcacaa atagcaggaa aagtaataga
     3001 gaaaaagtat aacttagaag tttcagaaga tgagattgga tatatagcaa tccatttcag
     3061 tggatattta gaaagaaata gcagtagatt ctattcaata aaaaaaatag caattatatg
     3121 tagtacaggt ctaggaacag caaaactatt gaaaataaga gtagaaaaac tgataggaaa
     3181 taatcctaaa attgatactc tttcaagttt taatttgaga aatattaact tagatgaata
     3241 tgatatagtt tttacaacta ttgatttaga cacatcaaat ataaatacaa tagtattaaa
     3301 aataaataca atatttgatg aaaacaaact aagagaacaa ttaaaaacag tattgtgcct
     3361 aagagaaggg aatattgaca ctactaattc tacaaattta ctaataaata atttgttaga
     3421 tgaagataaa tttatgatat taaatgaaaa gactatactt gcttcattag aaaaaatgat
     3481 ggaccatctt atggatttgg gatgcattga tgataagttt agaaaaaata tagtcaatag
     3541 agaagaaaaa tctcccacag tatttgataa aggtttatta tttcctcatt ctgtaaatga
     3601 aaaatcagat aagtttttaa tggcagttgg aattttagaa gaaccaatag aatatgcaaa
     3661 tagaagtata aaaataattc ttatgacaat gtttccatgt gagaataaga tagactcaga
     3721 cttattagta aaaatttatg aagaagtttt aaagataggc caagatgtaa aattaacgaa
     3781 taagataagc aaatgtagaa cttttttaga atttaaaaag gtgttactta aaaaccttat
     3841 ataactattg tacagcattt atagttgtta tacagattta aaaataatgt gatttttgga
     3901 aggtgtataa atatgaaatt attagtgatt ttatttgttg cttatatttt aaatactata
     3961 tttataatga agcagaataa attgtatttt aaaactttaa atagagctaa aaaaaagggt
     4021 gatatagtgt ctacaggcaa aaagaaaagc tatttttcta aaggaagtat agccattata
     4081 tcttcagatt cagatgggtt tataaagttt ggagaaattt taaaaggacg tacagtaatg
     4141 gctaaattta aagagattga agaaatagaa ggattagata tatatagtgc agagcaaaaa
     4201 tttaaagatg aagagagtat tgtacaagct ataaaattta taaaagaaaa aataaatgtt
     4261 tcttttaatt aactcaatat aaatataaat tatcaggagg aatgtcatga aatatgaagt
     4321 taagataagt gggataggca gtttagttaa agagttaatg gatgaaagca attgtcttat
     4381 tatatatgat gaaaccatta atgatgacga cttaaaggat atatctgtaa ttcattcaat
     4441 agcaacgtta aaaagtgatg ttgaaatagg agatacactt actataggga atagagatta
     4501 ttgtatagtt tcagttggtg atattgctca gaagactctt agagaaattg ggcattgtac
     4561 aatcaaattt gatggaaaat gtgaagtaaa tttaccagga gaaatacatg tggaaatagg
     4621 aaatccagat ataactattg gagatttaat aactatttct taatttgaaa ataaaattat
     4681 tataacagga ggaattggta tgttagattt aaacagaaaa cttgcaaaat tggaagagga
     4741 aggtaaaaaa ataaaagtag ctcttatagg agctgggcat atgggaaatg gaatggtaag
     4801 ccaaatggca aatatgaaag gtattgaagc ttctattgtt gtagatataa atttggagtt
     4861 ggctcataaa gcttttactg atgcaggaat agatgaagaa attatagata atgtaacaaa
     4921 tgaaaaggat gcagaaataa aattacaaga aggaaaagta ttgacatgta atgattttct
     4981 tgtggcttgt aaaactaagt caatagatgt agttatagat gcaactggag gtattgcaat
     5041 tggtgcagag atagcattaa actctatttt aaataaaaaa catatagtta tgttaaatgc
     5101 tgaaactgat tgtgttgttg gaccaatact taaaaaatta gcagatgatg ctggagtaat
     5161 atttacaggt tctgcaggag atgagccagg tgcagtaatg gaattatttg attttgcaga
     5221 tgcaatgggg tttgaagtta gagtgatagg taaaggtaaa aataataaat tagatttaga
     5281 ttgtaatcct gatacagtta gagaagaagc agaaagaaaa ggagcttcac ctcatatgat
     5341 agcttctttt aaagaaggaa ctaaaacaat ggtagaaatg gctttaatgt gtaatgcaac
     5401 tggatttgtg cctgatgtaa gaggtggtca tggtattgaa gctacagtta atgaagtacc
     5461 taagaaatat gcattaaaaa gcgaaggtgg agtattagat aattatggtg tagttgattt
     5521 tgtaaatggt atagcacctg gagttttcgt agtagtagcc cataaattaa aagcagttaa
     5581 tgatgaacta aaatatttaa gtatgggtga tggacctaat tacatattat atagaccata
     5641 tcatttatgt agtttagaga ctcctttatc agcagctatg gcagtcttag accataaggc
     5701 tacaatagta cctaaagctg gactagtagc agaagttatg actatagcta agaaagattt
     5761 gaaaaaaggt gagtttatgg atggctatgg agcatataca tgctatggta ctatagaaaa
     5821 atacgatgta gcaaaggcta tgaatgctgt acctattggt ttgataagta aaaagactaa
     5881 agttgtaaaa gatattaaaa aaggtgaagt tataacttac gatatggtag agatagaaaa
     5941 agatactact ttatatcatt taagacaatt gcaagaaaaa atatttgggt aagatttgat
     6001 ggtaatataa atagatatta gtaaaaaggg ttgatggtta tttttaaatc aaccctttta
     6061 aatttttata tattcttgtt aggatacttt ctttaaaagt atattatagg ttatttgtag
     6121 tcgtaaaaat tattttgaat tagttctgca attttatatt cacattgaaa caatattatt
     6181 aaaaatttta atataaaatg aattaaaatg accaattatg tattataata ttaactgtaa
     6241 tactaatata gttctattaa ttaaagaaat atcccccatt aatatataat aaatatcttt
     6301 aagcgagaga acttagtaaa aaatatatcg tattcaataa aaaatctgtt tacagaatgg
     6361 tatatatagt tatataatat actatataag atgcgaaaag ataaaaagta tagcacaatg
     6421 aaagaaggtg attattattc agcttaatga aagacaacta aaaattatag atatagttaa
     6481 ggaaaatgaa ccaataacga gtgaaagtat tgcctccagc ttgaatgtga ctcgtgctac
     6541 acttagatct gatttagcta tattaacaat gacaggaata ttagacgcaa gaccaaaagt
     6601 tggatatttt tattcaggag ttagtgaaat aaatttaatt ggtaaaagtg taaaagaaaa
     6661 aacagtagag gatataatga gcatgcctgt actagctaag aaggatgaaa gtatatatga
     6721 tgtaatagtc actatgtttt tatctgatgt agggagtata gttattatag atgagaatga
     6781 agagttatgt ggagttgttt ccagaaaaga cttgctaaaa gcaactatag gaggttctga
     6841 tataaataaa atgccaatag gtatgataat gacaagaact ccaaatgtag tcactttaac
     6901 taaaggagca agtgttttat tagcttcaag aaaaataata gaacatgaag tagattcgat
     6961 tccgattgtt gaatataagg gagaagacaa aaatcatatg agagttgttg ggagaatatc
     7021 aaaaaccaac ataactaagc tattcttaga aatagttgac aattaaggag gtatgctggt
     7081 gaaaaaccta attatatatg cagtatcaga ttcagtaggg gagactgcac aacaagttgc
     7141 aaaggcatgt atgtctcagt tttatgtgaa tgaaacttat gaaataaaaa gatttccata
     7201 catgattaat aaaggagtac tacttgaaac tttggagaat gcaaaagctg aaaacgcttt
     7261 aattgtatat acattagttg atgaagaatt atgcagtatt gttgaaagat attgcgaaag
     7321 agaaggtctt agttgtatag atttaatgac agatatacta agagaaataa gcaagagaac
     7381 aggaagaaaa cctaaaagag aagctggtat aattagaaaa ttagatgaat cttactttaa
     7441 gagagttgaa gctatagaat ttgcagtgaa atatgatgat ggaaaagacc ctagaggtgt
     7501 tcttcaagca gacataattt tagtgggaat atcaagaaca tctaaaactc ctttgagtat
     7561 gtatttagct aataaaaata taaaagtagc taatgttcca ttagttccag aaataccaat
     7621 tcctaaagaa gtgtttgaga tagatactaa aaaaataata ggtcttacta attcacctga
     7681 aaagctgaat gagataagaa cccaaagatt aaaagcttta ggattgtcca gtaaagcaaa
     7741 ctacgctaat ttagaaagaa tacttcagga gttagattat tcagaagaga taatgaaaag
     7801 aattggatgc ccagtaataa atgtttcaaa taaggcaata gaagaaacag caggtataat
     7861 tttagacata atgaaagaaa atggtctaaa gatatacaaa gaaatagaaa tttaataatg
     7921 tctattaata aaaatgatat aaattaaaat aaaaagatta atgggggatt taacaatgga
     7981 aactaagtat gtttatagtt ttggtgaagg aagcaaagac atgaaatctt tactaggagg
     8041 taaaggtgcc aatttagcag aaatgactaa gataggctta cctgtccctc ctggattcac
     8101 tataacaaca gaagcgtgta atgattatta tgttaacaat gaaagtataa gagcagagat
     8161 aataaaagaa atagaagagc atttagctac tttagaaaaa gacttaaata aaacattagg
     8221 atgcaataag aatccattat tagtatcagt tcgttctggt gcagtattct ctatgccagg
     8281 aatgatggat acaattctta acctaggtct taatgacaat agtgttgttg gcttagcaga
     8341 ggctacacaa aatgaaagat ttgcatatga tagttacaga agattcatac aaatgttttc
     8401 tgatgttgct atggaagttc caaaatataa atttgaaaac gtattagaca gagtaaaaga
     8461 agcaaaggga tacacagttg atacagaact tacaactgat gatttaaaag aaatagtaaa
     8521 agaatttaaa gcaatttata aaaaagaaat aaaaaatgat ttcccacaag acccaaaaga
     8581 gcaattaatg cttgcaatag aggctgtatt tagatcatgg aataatcctc gtgcaatcgt
     8641 ataccgtaaa ttgaatgata tagctcataa cttaggaaca gcagtaaata ttcaatcaat
     8701 ggtatttggt aatatgggag aaactagtgg tacaggtgtt gcatttacta gaaatccagc
     8761 tacaggagaa aataaattgt ttggtgaatt tttaatgaat gcacaaggtg aagacgttgt
     8821 tgcaggtata agaactcctc aaaatatatc aactcttgca gatgtaatgc cagctgtatt
     8881 cgatgaattt gtaaaaatta ctcatatact tgaaggtcat tataaagata tgcaagatat
     8941 agagtttact atagaaaatg aaagattata tatactacaa actagaaatg gtaagagaac
     9001 agcagcagca gcaataaatg tagcggttga tttagttgaa gctggaataa ttgatgaaaa
     9061 agaagctatc atgagaattg agcctaatca attagaccaa ttattacatc caaaatttga
     9121 agataaggca ttaaaagaag ctaaggttat agcaaaaggt ttaccagcat caccaggtgc
     9181 agcaagtggt aaagtttatt tcaatgctga tgatgtagta aaagcaaatg aaaaaggaga
     9241 aaaagtagtt ttagttagac tagagacatc tccagaagat atagaaggaa tggttaaagc
     9301 agaaggaatc cttacagcta gaggtggaat gacatcacat gcagcagttg ttgctagagg
     9361 tatgggtaaa tgttgtgttg ctggatgtgg agaaataaaa gttgatgagt ttaataaaga
     9421 aataagagca cttgatgatg tagttataaa agaaggtgaa tacatttcta tagatggttc
     9481 tacaggaaat gtgtacctag gagatgttaa gaaaacagaa gtatctttaa ctggaaactt
     9541 tgaaaaatta atgaactggg tagacaaaca caaatgtatg atggttagaa ctaatgcaga
     9601 taatcctaga gatgctcgtg cagctataga gtttggtgca gaaggtatag gtctatgtag
     9661 aactgagcat atgttctttg atgaagctag attgccagca gttagagaaa tgatattatc
     9721 taatacagta gaacaaagag aaaaagctct tgaaaaaata ttaccaatgc aaagagagga
     9781 ttttgtagag ttatttaaag ttatggatgg aaaaccagtt aatataagat tactagaccc
     9841 accactacat gaatttttgc ctcatgatga tgagactata gaagaacttt caaaatctat
     9901 gggaataaaa gtaagtgata ttaagaagag aatagttgat ttagatgagt ttaacccaat
     9961 gcttggacac agaggttgta gacttgcaat aacttatcct gaaatatgtg taatgcaatc
    10021 taaagctata atacaaggtg ctatagaagc aatcaaagca ggtgttaaag tttctccaga
    10081 gataatggtt ccattagttg gagaagtaaa tgagttaaaa ataataagaa aaatgatagt
    10141 agaaactgtt gacgctataa taaaagaaga aggtatagaa gttccttata ctgtaggtac
    10201 tatgatagaa atacctagag cttgtttaac tgctgatgaa atagcacaag aagcagactt
    10261 cttcagtttt ggtactaatg acttaactca aatggcattt ggatattcaa gagatgatgc
    10321 tggtaaattc ttaggtcaat atgtagatga agaaatatta gaaaaagacc cattccaagt
    10381 actagaccaa aatggtgttg gtaagttagt taagatggga gctaaattag gtagagaagt
    10441 taaaccagaa ttaaaactag gaatatgtgg agagcatgga ggagaaccat cttcagtaga
    10501 gttctgttat agtgtaggac ttaactatgt atcttgttca ccatttagag tgccaattgc
    10561 tagacttgca gcagctcaag catctataaa aaatcctaga taataataga ctgcaaaata
    10621 tgtaactaaa taaaaataaa atcccctatt gttcaatgta tcaaattaag tatttgtaaa
    10681 ttgaatatag gggattttta ttataaaaat ttaacttata tagttgtaag tgaaacgtat
    10741 ttaattatat tgcatatatt attgtagaga gaaatttcaa ctatcaatta aattgagttt
    10801 actggagggg ataaagttga caaagtgtcc taaatgtgga aaggaagtat catcacgtcc
    10861 aggtagtata tgcccacgat gtggatttaa agtagcagat gagagtctaa aaataagatg
    10921 tccagaacct agttgtaggg ctttggtatc taggaaactg gattattgtc ctaaatgtgg
    10981 atgcaaactt agaggatata atatttctga atttgttaac atggtgagat gtaaaataga
    11041 tgagacattt gataaaaaat aattaaattt agagttaagc ttttgagggt gtctcaaaat
    11101 gaacttttta gttcataagg cactcttttt gtatggaatc aaatatattt ttataattta
    11161 aacaataaaa aagaggctta tctctatttt gagatagcct ctataaaagt atacagtttt
    11221 ataagtaaat atatttttac taatctattt ctattatttg ccttctgaat attccatgac
    11281 taaagccacg agtgtgcata actatttttt aatcaaacgt atgttaaagt aatctttcta
    11341 tttaccagaa ttattttcca gtattaagta tccaaggttt ttctatacca ttttttagtg
    11401 agttataatt cacaataaga gagtcatagc tatttttagc atcttcatta cttaccactt
    11461 gttttttata atcagttaga gtaattaatc ctaaatcata tcttaattta gtatttttta
    11521 attgtttttc tgataaatca agttgttttt tagtaatctg aattgtatct tctaaagcaa
    11581 gtaattgagc atagctatca attaaccctt ttttaagagc ttttttactg tcttctaatg
    11641 ttactagacc attttcagta ttaaattttt gttgaagata atttgcataa gtagtaagac
    11701 cagcagcata agtctgtctt tcaagattgt atgcttcaac ttctttttta tatctatcat
    11761 aatctttttc atacttatcc ataagaacct tgtattcatc atcagttagg ttaccttgct
    11821 ctggttttgt aggagcaact ggtcttgtaa ggttaggtct atctggtaaa tctgctttgt
    11881 tctcatcttt atcattaaag ctttcttctg tacgttctaa aatcaattgg tcgtatttta
    11941 aatattcatt aaccttacct tccatatatg aatcaactga gccacctact ctaaaagatt
    12001 caaatctata ttctttatcc aattgataat tatttaaatc tatattagtc aataacttaa
    12061 aatagtcttg attgtttttt agttcttgaa gtttagcttt ttgagtattt tgtagtgttt
    12121 gaagttcaag ttcagctgat tcagtttcta aagatgttac taaccctaaa tcttttttta
    12181 atttcatatc tttaatttct ttagttttta tttcaagatt gttttttatc ttatctagct
    12241 cattttctct tgaaactaaa tcgttaaatt tattagtaat atcatttgct atttggtcct
    12301 ccataaagcc tttagcctgt tcactttgat ttttaagaag tccaatttgg tcgtatggaa
    12361 acgagttgtc acttccagta aaactatcaa actcatcttg tacttctaac ttttctttta
    12421 acatttttat ttcttctgat ttcattttta atttttcact atttttaagt gcagcactaa
    12481 cagcttctct agcagtcaaa atttcctttt cagatgttct tttatttgag tcacttaaaa
    12541 tagaattatc aactttgttt atagaagaag tattattatc tgcgtaaact gggattaaat
    12601 tggcagttat tataccaaca ccacagccaa ttgcaactat tttgctcatt tttttattca
    12661 atacaatcac cctccattct atttaaactt tagctttcaa ttttggtttc tttattattt
    12721 tacgttttat acttcctatt ttatctgtta tcttaccaaa tattttaggc attttgtctg
    12781 aaaacttatt ttcccattta tcaaatatta agtatatagt aggtataagc accaaagtta
    12841 taagtgtaga tacagttaga ccacccatta taactatggc tagtggttgc atagtttctc
    12901 caccttcacc aagagatagt gctgttggca acatacctaa aattgttgtt gctgtagtca
    12961 ttaagactgg tcttaatctt gctgcacaac caattgaaac caaaccaatt aaatcattgt
    13021 tacgatttgt ttttctaagt tgctctatgt agtcaataag aactatacca ttattaacaa
    13081 caataccgat aagtaagatt atacctagca taccgacagt acttaaactt tgaccactaa
    13141 ctagaagcga aagcacaact ccaacgaatg caaatggaac acagaacatg attataaatg
    13201 gtttactaaa tgattcaaac tgagctacca ttaccatata aactaatgct atagctatga
    13261 ccattgaaaa tccaagactt gccatagatt cattcatcat ttctgtttca ccaccaacac
    13321 ctatagagta atctttagga attaccattt tattcgtttt ttctagtgct agttttgtag
    13381 cagtaccagc atctaaattg tcaagtgtag cagttatatt tacagaaaaa tctccatcag
    13441 tcctagatat agattgtaga ccatctgcca tttttatatc tgaaaaagca ccaagaggaa
    13501 cttcttttcc agttgcagag tttactttta gttgctttat atcttctaag ctatcaatag
    13561 tatctgcttt aaattttagg tttacatcca ctttgtaatc atcaatagta gcagtggtta
    13621 cggagtcacc atttattgct gtacgtaaca tgcctgcaat ttcagcagta tttacaccat
    13681 attcttgagc cttatgttta tctatagtaa attgagcttc ttgtgttgta tctgtaagag
    13741 aagtttcaac atttctaaat ccttttatag agctaaattc tttttcagct tcttttccca
    13801 gtatttttaa tacatctaaa tcaggacctt tcatttctaa tgtgaagtct gctcctccag
    13861 ctccacccat aacactactg ttttgactta catttatttt acaatctggt acagtagcca
    13921 ctctatcaat tatctctttt tctatttcat cagtagttct ttttctattt ttttgagatt
    13981 ctaattcaat tgctatactt gctttactat tatttgaatc tgatgtcaaa gttgttatta
    14041 aagttttaat ttctggaata tctgagactt ttttctcagt catagataca tagtaatcat
    14101 tggattctag gctaagacct tttggaagtt ctatagaaat agataattca cccttgtctg
    14161 ctgcaggcat aaagtccata cctataaatc ccattccaaa taaggaagct acaaaaataa
    14221 gtaaacttga tattacaact aagcgttgat gtctcaaaca tacacttaat aattttttat
    14281 atgcttcact taccttatca aatataggac tatctttttc ttgcatcttt tgactatttt
    14341 ttcctctgct taattttgca aatacactag gaacgagtgt aattgcacct ataaaggaga
    14401 atgctagtga agctattagt gttttaccta atgccccaaa agttatttta gctatacctt
    14461 ctgtaaacat gataggtaag aatattgcta ttgtggtcaa tgttgatgca agtattgcat
    14521 ttgttacagt tcttgttccc tcaatagcac aatcatcaag actcaatctg ccacttttcc
    14581 tatatttaaa tatattttca attacaactg tagcattatc aacaaccata ccaactgaaa
    14641 ttaccaatga acttgcagta ataagattta aactttcacc agtaaagtag agaattgcta
    14701 tagcacctat tatagaaatt ggtattgcaa ctgcaacaac tagtgaagct cttacacttt
    14761 ttaggaaagc aaatattaca ataaatgcga taacagcact tataagcaag ttgctcatta
    14821 cactgtttat tgattcactt atgtatgaac ttgtatcatt agcaatttta attttaaatt
    14881 gaggattttc cttattcaat tcatctacgt atttgtatgc actctgcata acttttacag
    14941 tgttggcatc ttgttgttta gaaatatcta ttaccaaact gtcttttcca ttgtatctat
    15001 aaatactatc tttgtctgca tatccataat ctacactaca aagattatct aatctaactg
    15061 tatttcctcc ttttacagga atttgaatct gttttatatc atctagggat tctaatttat
    15121 caatcgctct taaaactatt ttatcctcac cttgagtaat tgaaccataa gggaatgttt
    15181 tattactaga ggataagaca ccttttatag tggataaact aactccataa tttgaaagaa
    15241 cagctgggtc agctattaca ttaacctgtg ctttttctcc acctttaagg tctgctgaag
    15301 taacaccatc tatggattct attttaggct gaattacatc ttcagcgtat tttcttaagg
    15361 cttgtgaact attttcattt cctccagata tgacaagttg acctatagcc tgtgcattca
    15421 tatccagctt taaaactgtt ggtttagctg catcatctgg gaggctggtc tgtaatgcat
    15481 caactttaga cctaacatca ttcattattt catccaaatt agttccaaat tcaaactgag
    15541 caactaccat agaaacactt tcttgtgaac ttgatatagt agattttaca tttgaaacgg
    15601 cagataaact ttcatcaact ttttgactaa cttgttcatc aacgtcttga ggaccagcac
    15661 ctgtccaagt agtcatgacc attacaactg gtatttctat atcaggcatt agatttattg
    15721 acattttttt atatccaata ccaccaaaaa ctattaatac taaaaagacc attatgattg
    15781 tgagaggacg tttaacggaa gtctgtgtta aattcatagt ctacttctcc ttttctacag
    15841 gaaatatctt agttccgtca gaaataagac ttatccctcc aataatcact gtatctttat
    15901 cacttacacc acttttaatt tctattttat cctctgttac aattccagtt tgaaccaagt
    15961 gtttctttgc tatattgtct tttgttgcta tgtatacata ttttttacca ttttcttcaa
    16021 atattgcttt tttaggtact gtaacagcac cattttcttt ttcaacactt aagtttactt
    16081 gagcagacat acctgcctta aatttgttgt ttgtattatt tacaagtact ttaacaggat
    16141 ataaagatgt ctgagggtca actactgaag gtacataatc tatttttcct aaaatccttt
    16201 ggtcttcaat tataacatct acatttccat ctttcttaaa tttacctata tctgattctg
    16261 ctacatttaa atcaacttct agtatattag gattagaaat aataaaagct ggttgtgatt
    16321 gtgttatcat ttcctttgaa tcaaagtttt tagctgttat tgtaccatct actggagatg
    16381 ttaatgttaa tttgtctaaa ttactcattg ctaaattata tgatacttga gcttggtcaa
    16441 gctgtttttt agcaagtgct ttagcttgag gtatactttt tgaagttgct gtatcgtatg
    16501 ctctttgagc agaatctaac tgttgttttg tttgctctaa agaaaattct atttttttaa
    16561 attggtctaa actaatgact tcctgttcat aaagaatctt attattgtca taatttcttt
    16621 gagactcctc atattgaatc tttgcattgt caagagctgt ttttgcctga tttacttgtg
    16681 cctctaatga accaccagtg gcacttgaat aacttgcttt tgctaaatct agtgaggctt
    16741 tactttgctt tacactattt tctagctctg gactgcttat actcaataat tcatctcctt
    16801 ttcttacatt ttgcccaagt tttacataca cttcttgaac tgtgccaaca gtctgtgcag
    16861 taacagctgt ttcatcttta acctttgtag ttcctgagaa agaatttgtg ttttcaattt
    16921 ctccacctac tgctttttga acagaaactg ctattggttt ctcaggttca gattcttctg
    16981 tttttccaca tccaactaaa aacagtgaac tacacattaa gaaaactatt aaatatcttt
    17041 tcatatctcc tcctattata tattgcacac tagcttgttg tagaatttaa agtgtcttat
    17101 aatttaagtt tagttttaga ctaattaaaa aacttccctt tattggcaat ttataattct
    17161 ctaatttgat tataaattta taattattat gagtcaa
//
LOCUS       DACXMZ010000072        16577 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.072, whole genome shotgun sequence.
ACCESSION   DACXMZ010000072 DACXMZ010000000
VERSION     DACXMZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.072"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            120..788
                     /locus_tag="JD016_09215"
     CDS             120..788
                     /locus_tag="JD016_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999812.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            790..1275
                     /locus_tag="JD016_09220"
     CDS             790..1275
                     /locus_tag="JD016_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999813.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1336..2457
                     /locus_tag="JD016_09225"
     CDS             1336..2457
                     /locus_tag="JD016_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAU4999814.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFVALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2738..3928
                     /locus_tag="JD016_09230"
     CDS             2738..3928
                     /locus_tag="JD016_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAU4999815.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENDTYRMDYDDLEE
                     KAKCNNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4062..4317
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4376..5377
                     /locus_tag="JD016_09235"
     CDS             4376..5377
                     /locus_tag="JD016_09235"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAU4999816.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5481..5870
                     /locus_tag="JD016_09240"
     CDS             5481..5870
                     /locus_tag="JD016_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HAU4999817.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            6031..7239
                     /locus_tag="JD016_09245"
     CDS             6031..7239
                     /locus_tag="JD016_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAU4999818.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7325..8401)
                     /locus_tag="JD016_09250"
     CDS             complement(7325..8401)
                     /locus_tag="JD016_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAU4999819.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            8630..9157
                     /gene="hpt"
                     /locus_tag="JD016_09255"
     CDS             8630..9157
                     /gene="hpt"
                     /locus_tag="JD016_09255"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAU4999820.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9246..10178
                     /locus_tag="JD016_09260"
     CDS             9246..10178
                     /locus_tag="JD016_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAU4999821.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNMSD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10240..11466
                     /locus_tag="JD016_09265"
     CDS             10240..11466
                     /locus_tag="JD016_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAU4999822.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11550..11771
                     /locus_tag="JD016_09270"
     CDS             11550..11771
                     /locus_tag="JD016_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HAU4999823.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            11960..12130
                     /locus_tag="JD016_09275"
     CDS             11960..12130
                     /locus_tag="JD016_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999824.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12223..12684)
                     /locus_tag="JD016_09280"
     CDS             complement(12223..12684)
                     /locus_tag="JD016_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999825.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            12867..13334
                     /locus_tag="JD016_09285"
     CDS             12867..13334
                     /locus_tag="JD016_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999826.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            13371..15140
                     /locus_tag="JD016_09290"
     CDS             13371..15140
                     /locus_tag="JD016_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HAU4999827.1"
                     /translation="MSMTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPT
                     KACVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKI
                     IYVENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDID
                     FISSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSK
                     QACNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNL
                     KPCSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLY
                     EHNEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYAT
                     KVDDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFD
                     KMGVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDAT
                     YENNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKL
                     DLKNFSSKDNYDDDILIVMLKDR"
     gene            15142..15756
                     /locus_tag="JD016_09295"
     CDS             15142..15756
                     /locus_tag="JD016_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAU4999828.1"
                     /translation="MIMDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECG
                     LSCKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSI
                     SAKLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDF
                     FFKSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            15828..16397
                     /locus_tag="JD016_09300"
     CDS             15828..16397
                     /locus_tag="JD016_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HAU4999829.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         6482 a         1680 c         2932 g         5483 t
ORIGIN      
        1 ggaataaatt tacaataaaa aaataaatac aaaggcttta tcattaaggt attggcataa
       61 gttttgctta ttaaacaagt atactaataa aattcattta aacaggaggg agaatttata
      121 tgagtaaatt agaatcaaca acagaaaaag ttgaggtaaa atggtatggg tatgtagcat
      181 taatattggg agcattattt ttttcaggaa tatttaaaga tgcaccagga ccattaaaag
      241 tattggattt taacaatgta ttaggttctt ttggaacatt gggaacaatt aatgatggtg
      301 taggtacatt agctgcaaac tttagaggtg atggaggtac tggccctcga gatggatggc
      361 tatatgcatt gacattaata ccatcagtta tgtttgcact tggagtagtt agagttatag
      421 accacttaga tggaatgaag gcagctcaaa agcttcttag tccactatta aagccattat
      481 tagggttacc tggatttgca ggactaactt tgatagctag tttacagtct acagatgcag
      541 ctgcatctat gactaaagaa cttaaagatg atgggtatat agatgaaaaa caaaaagctg
      601 tattttgtgc atttcaattt tcaggtgcgt ctgcaattac gaatttcttt gcttctggag
      661 cagcactatt tccatttata ggagatgtac caatatttat accacttgct ttaatattaa
      721 ttatgaagtt tgttggggcg aacttactta gattatattt aaataaattt gacaaggggg
      781 aagcttaaca tgagtgaggt agcaaaaaaa gaagtaaaag ttaaaaaagg aaatgtgata
      841 gatgcgttta ttggaggagc tagaaatgga ttccaaataa gtacaaattc aatggctcca
      901 aatgtcatct tcggttttgc tataatttct gtgttcaatt taacaggtct tttggatgta
      961 atagggacaa tatttacacc tgtaatgagt atctttggac ttccaggtgt tgcagcaaca
     1021 gctataatga caatatttat atcaatggga ggagcatctg gagtaatcgc aggtttattt
     1081 acagcaggtc aaataagtca agcacatgtt gcaattctat tgccaatgtt attgctttca
     1141 ggagcacaga tacaatttat ggggagactt ttaggaacag ctgagttaaa aactaaatat
     1201 tatccacata tgtttgttat agcaacttta aatggttgta ttgcaatgct tataatgaat
     1261 tatttcgtag tataggtaat tataataaaa atttaatata aatggaaagt ttaaatttta
     1321 aggaggagct agattatggg aactaacaaa gattatattg agtatgtaca taaagtgtat
     1381 gattatctac acactatacc agaaaaagga tttgaagaat ataagacatc agagtttgtt
     1441 ttaaaagagc ttgaaaaaat ggggtttgaa aatattaata aaggagttgc aggaactggt
     1501 attatagtaa ctatagattc taaaaaagaa ggaccagtat ttgctataag agcagatatg
     1561 gatgcattgg aatttatcat agatggagaa aagaaaatga tacatgcttg tggtcatgat
     1621 ggtcatactt ctatgctttt aacagctgca aagttaataa aagagagaaa tttagtaaaa
     1681 agaggtaagt taaaaatact ttttcaacca ggtgaagaaa aattatatgg agcattaaga
     1741 gtaattgatt ctggtttgtt ggatgatgta gaagaaatgg tagggatgca tttaagacct
     1801 ggacaagatt gtaggttggg aaaggcgata cctgcaatgt accattgtgc tagctgtata
     1861 atggaattta cgattaaagg tatcacttgt catggttcta aaccacattt aggagtaaat
     1921 gcagtagatg ctgtctgttt agcagtgaat gcaataaatg caataagagt aaatccagct
     1981 ataccaaatt ctataaaagt aacaaatata tcagttaagg gagaaactta taataatata
     2041 ccagatgaag catttgtggc tttagatata agggcagaaa gtaatgatat aatagaagaa
     2101 ttaaaagaaa aagcaataca tgctgtaaaa actgctgtaa gttctataaa tgcagaagct
     2161 gtactaattt ccaaagatgg agtaccagca gcagaatttg atgaagaact tgtaaacttg
     2221 gctgaagaaa ctataaaaga tgtattggga gctgatgcat ctataggtat atttaaaaat
     2281 tctggtggag aagatttcca ttattataca caaaaactaa aatgtaaaac tacatatatt
     2341 ggattaggag ctgatgctac accaggattt cataatccaa atgtaacatt tgatacgaaa
     2401 gctttagaat atggtgtaga tatatggtgt agattggttg aaaagagatt gggataacat
     2461 ccaaactaat aattctacat ttaaaattat aattatagaa gttatagaaa aagattgctt
     2521 atgttaatta gaaaataatt aatatgagca atcttatttt gatataatga aactgatttt
     2581 ggaatattga agaaatatga tgggaaagta attatatttt aaatctattg gacaaaaaag
     2641 tgatattatt ataatattaa gaattgtaaa ataataccaa agtgcatcta gtaatagact
     2701 taacaattat aaaaatttta ttcttaggag ggaaaaaatg agtaagtata attttgacag
     2761 agtaatagat agagtaggaa cagattgtgt aaaatgggat tttagaacta attgttcaac
     2821 aaaagcacaa aaagatggtt tgcctttttg gattgctgat atggattttg aatgtgcaga
     2881 accaataata gaagcattac gtaaaaaagt tgaacataaa atatttggat atagttcaaa
     2941 tgatagtgat aaatacttta atgcagtttg tgattggtat aaaaggagat ttaactggga
     3001 aataaataga aaagatatta tttttagtcc tggtgttgta cctgctgttg ctattttagt
     3061 aagaatatta actaattcta atgaaggtgt aatagtccaa aaaccagtgt attacccatt
     3121 tgaagctaag ataaagagca ataataggga cgttgtaaac aatcctctga tatatgagaa
     3181 tgatacttat aggatggatt atgatgattt agaagaaaaa gctaagtgta acaacaataa
     3241 agtactgata ctttgtagtc ctcacaaccc tgttggaaga gtttggagag aagatgaatt
     3301 aaaaaaggtt gtagaaatat gtaaaaaata tgacctatgg ataatagctg atgagataca
     3361 ttctgattta attagaaaag gatttaaaca tactccttta cagtcattat gcccagagta
     3421 caaggataaa atagttacat gtatagctcc aagtaaaaca tttaatctgg caggaatgca
     3481 gttgtctaat ataatagtga ataatgatga actaaaaaag aaatatcaag aagaggttac
     3541 agctgttgga gttgcaactt caccaaatcc ctttgctatt gtagctacaa tagcagcata
     3601 taatgaaagt gaagactggc ttaatgaatt aaatgattac ttagataata atatacaatt
     3661 tattgatgaa tacttaaaag aaaacctacc aaaagtaaaa cttgtttatc cagaaggaac
     3721 atatctagca tggttagatt ttactgctta tggattaaat gaagtagaac ttgaggattt
     3781 aatgtttaaa aaagctaatg tattatttga tgaaggttat atatttggaa aagaaggaat
     3841 aggatttgaa agaataaatg tagcttgtcc acagtcttta ttaaaagaat gtatggacag
     3901 attaaagact atatttgaaa atttataaag tataggtaaa aatttataaa gtgtttaaaa
     3961 ataaaaaaat aataaacttg acaaatttta actaatcact tatcatacta ttatgaataa
     4021 atagaaaaca taagagagct aaattttatt gaattaaact cttaaagact ttgaaaggaa
     4081 gagtagttat agaatgtaat taaagcgagt tggtgatggt gtgagaccaa tatgaagtct
     4141 ataatgaaga acatcctgga gttgctaacc gaaattttaa aagagtaggc ttagtcggaa
     4201 ccaaaccgtt atcatattgg ggtgtatcga gtaatatctg tacattgtga gctgggtttg
     4261 aatttatttc aaactaatca gggtggtacc gcggaaaggc aacctttcgt cccttttata
     4321 tagggatgaa gggttttttt aatggaaaaa tatataaata tagggagtga atgatatgag
     4381 cacaataata ccaaaggatt ataaaagtag tttgaacgta attgatacac aaatagctat
     4441 aaaaaaatta aaagattttt ttgaaatgag actaagtaat gaattaaatc ttataagagt
     4501 ttcttctccg ttatttgtat tgccagaaac aggtgcaaat gataatttaa atggagaaaa
     4561 ggctgtaagc tttgatatac catttatgaa taaaaatgca gaaattgttc aatcacttgc
     4621 aaaatggaaa agattagccc ttaaaaaata tgggtttgaa gttggtagtg gtttatatac
     4681 agatatgaat gcaataagaa aaaatgaaga attagacaat atacattctc tttatgtaga
     4741 ccaatgggat tgggaattgg ttatagataa aagtagtaga aatgaaaaga ctctaaaaga
     4801 tgttgtaaaa agattgtatg gagtatttaa agatactgaa atttttgtat gtagtatgta
     4861 tgaaggtata aaagaaatat taccagaaga aataactttt ataacatcac aagagttaga
     4921 agatatgtat ccagaattat caccaaagga aagagaagat aaaattgtaa aagagaagaa
     4981 agcagtattt ataactcaaa taggaaaaac attaatatca ggagaaaaac atgatggaag
     5041 aagtcctgat tatgatgatt gggaattgaa tggggatata ttattttaca atcctgtttt
     5101 agattcagca ttagaattat cttcaatggg aattagagtt gatgaagaat ctcttgatag
     5161 acaattgaaa cttgctggat gtgaagagag aaaagagttt gactatcata agatgctttt
     5221 aaatggcgaa cttccgtata caattggtgg aggaattggt cagtcaagaa tatgtatgta
     5281 tttcttacaa aaagctcata taggagaagt acaagttgga gtatggccac aggatatgat
     5341 tcaaaattgt agtagtgcag gaattgaatt attataattt aaatataaac taattgataa
     5401 aatttatatc taatatgttc atcaaattaa ttatatcggt tataatgtag aataacctat
     5461 aattataaga ggtgataaat atggagtttt taaaaataat cattaatata gttttagata
     5521 ttttaaaaaa aattttggtc agatttaaaa atgctaaatt tggtttggtt tttgtatttg
     5581 atttacttaa actacctgat tttatgacag ataaaagaat caatatagtt gataaaataa
     5641 aggtaatttc tgtattaatt tttactatat cctactttgt atctggggtt gatataattc
     5701 cagagatgat tgcaggggca tttggtttta tagatgatgc tatagtttta atttggagta
     5761 taggtatagt gaatgaagaa attaataagt atagagttat cgcaaaaaaa gataaacatt
     5821 caaatataat tgaaaatgta gaattttcta taaaagatga agaagaataa caatttatat
     5881 atgttattct tttttttatt aacaaaaaat ggttaaattg ttgacggaga ggtaaaactc
     5941 tattatcata ataagtggat atccaatatt taactaagta ttcaatgtgc aagaatgctt
     6001 aatttaaaaa agttatggag ggtaaatatt atgaagaaaa aaataggttt agtgcccaaa
     6061 ctaatcatcg gtatcatagt cggtatttta atcggaagtt atgcaccaga aataatagta
     6121 caaatattag taacagttag tactttattc tcagcattcc taaaatttgt tataccattt
     6181 ataattatag gttttgtaac agcaggtata gctgatttag caacaggtgc aggaaagtta
     6241 ttggggataa ctacgggaat agcatatggt tctaccttaa tagcaggatt attatcattt
     6301 gcagtatcta cattaatctt cccaaatttt attgatgcaa gtgttgcaag tcaaattgga
     6361 gacccagagg caggaatgtt agctccaata ttcacaattc cattagagcc tatggtagat
     6421 gtaacagctg caatcgtatt tgcatttgta atgggacttg gaatatcagc tttaagaaat
     6481 catggaaaag gagagacttt atttaatttc ttccaagaat ttcaaggaat cgtaacgaaa
     6541 acgttgtcaa cagtgattat acctttatta cctttataca tagcaggaac ttttgcaaat
     6601 ataactatag caggggaagt atggacaata ttaggggtat tctggaaagt atacttagta
     6661 gtaatacctt tacattttgt atatatggca tgtcaattca cagcagcagg tgtattttca
     6721 ggaaaaaatc cagtaagaat gttaaaaaat caagtacctg gatatttaac agctatagga
     6781 actcaatctt cagcagcaac tataccagtt aatgttggtt gtgcagaaaa aaatggagta
     6841 agtaagcaaa taagagaatt tgttgtacct ctttgtgcaa caatacattt atcaggtagt
     6901 atgatatcaa taacaggatt tacaactgca gttttaatga tgaatggtat gccacatgga
     6961 ataggtgtat tattccctta tattgcaatg ttaggtatag caatggttgc agcaccagga
     7021 gcaccaggtg gggctataat gagtgcactt cctttcctac caatggttgg aataccatca
     7081 gatggtgggc ttgcaagttt aatgatagct ttatacttaa ctcaagacag ttttggtaca
     7141 gcatgtaatg tatcaggaga taatgcaata gcagtagtag tagataaaat aaatgatgga
     7201 atgagtaaaa aaggaaacaa agaagagcaa agtgcttagt tttttaataa ttaaaaatga
     7261 gttatctcaa atagagatag ctcatttttt atggtataaa aaattataaa ataaagtgat
     7321 ttttttattt aaaaattgct ttaagagatg atattaataa tttattttca ttaggtttta
     7381 atatacatac tctaaagaaa tactcatcta gaccttcaaa tgaacaacaa tctcttatta
     7441 ttattttttg tgggagcagt tgctctcgaa gagatttagc agtcaattcc tttgtcttga
     7501 ttttacacaa tatgaaatta cctttagtat tgtatatatc taagcttttt atattcttaa
     7561 gctctttaag caggtaatct ctttcagtgt tcattaagga aatagtattt gatataaaat
     7621 ctaaatcact aaacatgatt tcccccattt tagaagctat tatgtttata ttccataaat
     7681 ctagattttt atttatttca tttttgacat tagtgttaga tataaggcca tatccaagcc
     7741 ttattccagg cgttgaaaaa aattttgatg tacctcttat aacgaataaa ttagaataat
     7801 catcaactaa ttgagtacaa gagtatgtat ctgtatcagt aaactctaca taagtctcat
     7861 caatcattaa aaatgagtct gtatttttta aaatttctct cacttctacc ttagtaaatg
     7921 caaatccagt tggattacta ggattacaga ttactaccaa atcataatct ttagcattga
     7981 tagtttttat aagattttct aaatttatat gaaaattatc ttcttctttt gcaaagtatt
     8041 tttctataga acaattaatt ttagataact ctttttcata ctctgaatag gctggagaga
     8101 gtaaaagagc ttgtttagga ttaattgtat gtataaatga tgaaattagt tctgtagctc
     8161 cactgccaag tactatatta tctatagaac atttgcagta attggatata gaagttttta
     8221 agtctatgta atcagggtca ggatacatag ataccatatc tatattgttt acaatgtatt
     8281 gtttggctaa acttgatgta ccaaaaggat ttatatttga gctaaaatcc ataaattctt
     8341 cctttgaaaa gccatactta ctagataaac tatataaatt agctccatgg tttgtgctca
     8401 taaaagtacc tccaaaaata aaattatata atcgataaaa ataactgatt aatatatgta
     8461 catatagatt ataatacaaa ggaatcgtta ttccaaattt ttaatgtaaa aagcaaaaaa
     8521 gtatggataa gctgttaatt taggtttaaa aatgatgaaa aagattttaa ttgatatttt
     8581 ggtatgctat aatatataag ttaatagaag gaaagcggga ggatacaaaa tgtacaaagt
     8641 tacaggaaaa atgttgacag aagaacaaat aaaagaaaaa gtttatgaat taggaaaaaa
     8701 gatagaagaa gactttaaag gcgaagattt actagtggta ggtatactta aaggagctag
     8761 tgtatttgtt tcagatttaa taagatgtat agatttggat gtaaatatag attttatgag
     8821 tgttacaagt tatggaaact caacagagtc ttctggaaca gttaaaatat taaaagattt
     8881 agatgtagat attgaaggaa agaatgtgtt gattgtagag gatataatag attctggttt
     8941 aacattaagt aatctagttg cagctttaaa aactagaaat ccaaaatctt taaaactatg
     9001 tactctactt gacaaacctc agagaagaaa agctaatata cctgtagact atgttggatt
     9061 tgttatagaa gacaaattta ttgttggtta tggaatagac tatgctgaaa agtatagaaa
     9121 tttaccttat ataggtatag ttgaggatgt agaatagatt taaatgtgaa tcaaaagcac
     9181 taatttctaa gggagttagt gctttttagt taattatata aaattttagt aaaggagaca
     9241 tctatatgga tattcagtta aatatattat tatttttcca aaatttaaga aatccaattt
     9301 tgaactttat atttcttgtc tttacaataa gcacagaagc accgttatta atattaatta
     9361 caacaattat atactggtgt ataaataaaa aatgtggaca gaaaatactg tttgctataa
     9421 ttggaaattt tgttgtaaat ttaggcatca aagagtttgt aaaagctccc agaccaatag
     9481 gaataaaagg tcttgaatca ttgagagttt ctactgctgg tggttattct tttccaagtg
     9541 ctcatactca aactgcaaca acattttggg ttagtattat gactatattt aaaaagagat
     9601 atcttcattt aataggtgca gttatggttt taggagtagg attgtctagg ctgtatttag
     9661 ctgttcattg gccaatagat gtgatagcgg gttggatatt aggaatattt tttacagttg
     9721 tatttgtaaa aatatttgac tatattgatg ataataaaaa ttatatttta cttgtagttt
     9781 tacttatacc atttataata gtggctatat ttctaaatag ccctgaatat tttgaaaaat
     9841 ttggtataat agtaggattt gtatttggat atatggttga ggatagattt gttaaattta
     9901 atacagataa taataacaaa aaaataaatt ttagtaataa aaataagggg agtaaaaata
     9961 taattttttc aagaatatgt aggtttttgg ttgggatagt aacaatagga atgttatata
    10021 ttggcattaa gttgtttatt tcaatgttaa tagtcacttt aaatatgagt gactctacta
    10081 tgagtataat ttttatgaat tttattaaga atacagtagt tgttttttat ggaatagcag
    10141 gagctccagc attatttaaa cttttaaaat taaattgatt ttaaggtata gagtcaatag
    10201 tttaaattaa aaaaattaag ggtagaaaag agtgattatt tgaagaaggt agtaataata
    10261 ggtggtggac cagcagggat gatagccgca tcaactgcat gtgagaaagg ctatgatgta
    10321 acattaattg agaaaaatca taaattagga aaaaaacttg caataacagg aaaaggtaga
    10381 tgtaatatta ctaacgcttg tgaaatagag gaattaatag aaaatgtacc tacaaatgga
    10441 aaatttcttt atagtgcttt ttatactttt acaaatgatg atgtaataag tatgtttaat
    10501 aatcttggag taaagactaa aacagaaaga ggaaaaaggg tttttccaga gagtgataaa
    10561 gcatttgata ttgtaaatgc tcttgaaaga caacttaaaa gtaagaaagt aaatatatta
    10621 ttaaattcga aagtagaaaa aataatatct aaaaataata aaattgaaaa agtaatttta
    10681 aacgataaaa aagaaattaa gtgtgattca gttgtagtag caactggtgg attaagttat
    10741 ccacttactg gttctactgg tgatggatat aaatttgcaa tttcacaggg gcacacaatt
    10801 atagatacaa aaccatcttt aattggtata gaagtacaag aaagttttac aaaagattta
    10861 gaaaaattat cattgagaaa tgttgaaata agggtgttta atagcaaaca aaagaaagtt
    10921 tatagtgatt ttggagaact tgaatttact aaatttggtt tagatggacc tataatcaag
    10981 tctgcaagtt gtagaatgaa agatacaaga aaagaaaatt acactatatt attagattta
    11041 aaacctgcat tagatgagga aaaactggat aaaagagttc aaaaagattt tcagaagtat
    11101 acaaataaaa aatttgaaaa agcattagat gatttacttc caaaaaaact aatacctata
    11161 ataataaact taagtgaaat taatccaaat acggttgttc accaaatttc tagagagcag
    11221 agaaaaaact tagtccattt attaaaaaat ttaaagttta cagtaaagag atacagacct
    11281 attgaagaag ctataataac ttctggaggt gtaaaagtaa acgaaataaa ttcaagcaca
    11341 atggaatcaa aattagtaga aggtttattt tttgctggag aagtaataga tatagatgca
    11401 tatacaggtg gattcaattt gcaaatagct ttttctactg gatatttggc aggttttaat
    11461 tgctaaaaat tacctgaatt aaaaatgttt aaaatcacat aatattatca gcaacacaaa
    11521 ataaattaaa cagaaggaag gtaatcaaca tggcaagtaa caataacaac aacagaacag
    11581 tagtaccagg agcaaaagag gctttaaacc aaatgaaatt agaaatagct aatgaacttg
    11641 gtatgagtaa ttatcaacaa gttgataaag gaaaccttac tgcaagagaa aatggttatg
    11701 taggtggata catgactaaa aaattagttg aaatggctga acaacaaatg gctggaaaaa
    11761 gcaacagata gttataaata attatttata actattggct attagtttag ctagagctat
    11821 ctcaaatata gaaagtaatt tctaaattga gatagctttt ttttattaaa ttattaaata
    11881 gatatatttt tatgtaataa attttatgta tttggataaa ataatacaaa tgaatatgtt
    11941 acataaggag tgatgtaaaa tgagtagtgt aaattttgag gatttaaaaa acaaatttat
    12001 taatagtgac ttagatgaaa aaataagaat atatacaaca acagaaggtt tatcagtaga
    12061 acaatttaaa gagctactaa aatattatcc aatacaacat ttatctaaac tagaaaaagc
    12121 acttggatag cagtatttaa tccaaaatta aaaaggcaat ttatttaata ttaaaaatat
    12181 tttttattaa ataaattgcc tttttataat aggtttaaat tattatgctt ttgatatata
    12241 attatagatt ctctcatttc caagttcaat agacccagta ttaataagag taactattcc
    12301 ttctatatct ctagcagtta agtatttata tggaattaat aaattataac cagtattact
    12361 tagtaagttt tttaattggg ttatttgaga gatattgaaa cattttgcag ttttagtaga
    12421 tttttttctt aagttagata aattttcatt ttgaatattt attaaagttt ctatatagtt
    12481 tctataagat atcttaaagt ttgtctcatt gactattttt tcattaagta tactactgat
    12541 aaaatcttga gacaatatat tatctgcaaa tttatctgct gttaattttc cagatgttat
    12601 gcctttttga tattttctaa gaattttttt agtatagcag tatattttat ttctcgtttc
    12661 taaatcgagt tgttctattt gcacaaaacc acttccttct aattataaaa atatttatta
    12721 aaaatctata cttaactaat aaagcttcaa taacttattt acaataaata ctaaatcata
    12781 aaatatttgt tattttttat tattaccaag tataaaaaat attataattg taaaacttac
    12841 aaatatatgt aaaatattta ggagtgatga aaatgttaaa tgggataata aagctttttt
    12901 attctgaaat tgtaaaaaca aagattaata acttagattc aagtggatat tctttaagag
    12961 atgaatgtaa agaaatcaga aatataactc taaagataga tagtgtattt tttaagcttt
    13021 taaagttagc ttttggttca atattgctta tggtagcttt taatttaaac atggttttag
    13081 gtataggagt gtttttagtt ggtgtagttt atatactata taaaagaaat atggaagaac
    13141 aagtaaaaga gcatataaat aatgtgaaaa ataatatcga catctctaaa ataactttta
    13201 tggatgaaaa agggagaaca ggtctaaatg cattaattac attacttctt ataggattat
    13261 tgacaggatt taactggata ttagttgcta gttttgtcgt tgtttttgtt tttactataa
    13321 agaatgtatg ttaaaatact aataaatagt ttaatatata gggggagaaa ttgagtatga
    13381 ctaatggcag ttataaaatg aagaatttag tggaaatatc cgctatggtg actgaatcta
    13441 cagatttttt taatataaaa gatgacataa tagaaaaaat gttagaagtt gttcacccta
    13501 caaaggcatg tgtgaatcta ttttataaaa atgattctaa atatgcctac ttagtatgtt
    13561 cgcaaacctt agaatatgta cctcaaattt ttccaataaa tagtcttaaa ggtgcaaaaa
    13621 ttgattttga cacatatcca gagtatatac atgaggctgt aaatgaaaaa aagataatat
    13681 atgtggaaaa tgtatttgaa gattctagag cagaaggtga acgagattta gcaaaagctg
    13741 aaggttatat tgggagaata gtttttcctt ttataataaa taacgtagtt gttggattta
    13801 tgacttgttt tttaaaagag gatgattata taacagaaca agatatagat tttatatcat
    13861 ctgttgcatc actcttaagt ttatcaatag aaataactaa taaaaataat aatgtacaaa
    13921 ttttaattga taaactaaga ggctcaataa gtgccataaa tgaagctact aaaaaactat
    13981 accttaataa gagtattgat ggatttttgg aaaatttaag taaacaagcg tgtaatatta
    14041 ctaatagtaa agaagcttta ataattataa atagtcatca taataaggat gctatagtta
    14101 gttgttataa tgttgagcaa aaaaagaaaa caaatttata tcctatgatt gatatgtttt
    14161 taaagaaaga gtcattgggg gggtatctaa ataatttaaa accatgctca aaaaagggaa
    14221 taaatttgga aagttatata tattacaaac ttcaggataa aaatgatact ataggatgta
    14281 tagtttgtgc aaatagcaaa aactatacta gtgatgattt aaatattctt agtatattgt
    14341 ccaaacaagt ttctgttggt atgcaattat atgaacataa tgaaaaggaa gtaaaacaca
    14401 aagttcttga aaacgagttg aacatattaa ataaacaaca acagcttatt atggataaag
    14461 gtaaaatgga ttgtaatgat gagaaagaac tttattttta ccataaacca gcaagggtag
    14521 taggtggaga tttttattat gctacaaaag tggatgatga acatatagtt tatattgttg
    14581 ctgatgtaat gggacatgga atggtatcaa attatgttgt tgcaatgata aaaggtgctt
    14641 ttaaagtatt atgtaatcaa tacaatacac caagagaaat aatgacaaat ttaaatagga
    14701 tgttatatga tgaatttgac aagatgggag tatttacaac ttgtttgata ggtattatag
    14761 atactaaaga aaatatgatt actgtgtcta atgcaggtca ctatagtcct gtagttgtaa
    14821 agaaagatgg cacaatagaa aataatttaa attgtaaaaa gggcatacct attggaataa
    14881 tggaagatgc tacttatgaa aataatactc tttcaatgga agattatgca atggtatgta
    14941 tgtacactga tggaatttta gagataaaaa attcatcaaa agaagagtat ggtataaacc
    15001 gattagaaaa ctttctaaaa gaaaatttta gattaaatca acagttaata gtagaaaatc
    15061 taaaattgga tttaaagaac ttctcatcaa aagataatta tgatgatgat attttaatag
    15121 ttatgttaaa ggataggtag attgattatg gattcaaacg ataaaaaagg acttaaaagt
    15181 ccagagatta taataataaa tggaagtcca agaattaata aaaattgcag ttttatagca
    15241 aatgaagtaa aagctatgct agaagagtgt ggattaagtt gtaaaatatt taatatctac
    15301 aatatggata ttgaatattg cacagcatgt ggattttgtg aaaaaacagg gtattgcaag
    15361 tttaatgatg atatgaagcc attatataaa atgtttgatg attcaaaagg aactatagta
    15421 ataagtcctg tacattttga ttcaatatca gctaaattaa agactgttgt tgatagaact
    15481 caagctatat atgcaagtaa atatgtatta aataaacctt caattgatag aagtaagaaa
    15541 cggataggaa tgtatattgc agtggggggg agtaaatcat atgattcaca gtttaaaggt
    15601 ggacagattg tgatggattt tttctttaag agtataaata ctaaactttt acacaatata
    15661 tatttaaaca atacagatga tgtatcttat atagataatc aagaatttaa agatataata
    15721 tataaaaatt taaagatata tattgattca atataatttg attttgtagg taatattatg
    15781 tataattgta ttgcaataat ttaaataatt aaaaggaggg aatttcaatg aaattaccaa
    15841 tagcattatc taataaacat gttcatttga gtcaagctga tatagacgta ttatttggag
    15901 caggtcatga gttaactaaa tttaaaccat tatctcaacc agggcaatat gcctgtgaag
    15961 aaaaagtgga tttagtagga ccaaaaggaa ctttaaaagg agttagagta ctaggaccag
    16021 ctagagctca tactcaagtt gaaatatctt tagcagatgg tttcccatta ggagtaaaag
    16081 caccaataaa agaatctggt gatatagctg gaactccagg tgttaaatta gtaggacctg
    16141 ctggagaagt tgaaatgaaa gaaggagtta tagttgcttc tagacatata catatgagca
    16201 atgaagatgc tgctaaattt ggtgtaactg ataagcaaat agtaaaagtt aaaacatctg
    16261 gaccaagagc attagtattt gaaaatgtat tagtaagagc tagtgacagc tttaatctag
    16321 aaatgcatgt tgatatggaa gagggaaatg cagcaggcgt taaaaatgga gatttagtag
    16381 aattaatagt tgattaatat tataaataga ctttatgcta gtagatattt tatttgctag
    16441 catattttta aatgtaataa atttctatag gataaattta atcgattaga ttaatttata
    16501 aaataaaggg gtattataaa aaatgtaata aaaacttaca aaaatatgat gtaatatggt
    16561 aaaattatat atataaa
//
LOCUS       DACXMZ010000073        16157 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.073, whole genome shotgun sequence.
ACCESSION   DACXMZ010000073 DACXMZ010000000
VERSION     DACXMZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16157
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.073"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            243..776
                     /gene="pyrR"
                     /locus_tag="JD016_09305"
     CDS             243..776
                     /gene="pyrR"
                     /locus_tag="JD016_09305"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAU4999830.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            1060..2271
                     /locus_tag="JD016_09310"
     CDS             1060..2271
                     /locus_tag="JD016_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAU4999831.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFI
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            2570..4222
                     /locus_tag="JD016_09315"
     CDS             2570..4222
                     /locus_tag="JD016_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HAU4999832.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCGHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEAKNKPKVSKEMNIK
                     IQDGMDIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(4437..6212)
                     /locus_tag="JD016_09320"
     CDS             complement(4437..6212)
                     /locus_tag="JD016_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAU4999833.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDNISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDALHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            6508..7542
                     /gene="ltaE"
                     /locus_tag="JD016_09325"
     CDS             6508..7542
                     /gene="ltaE"
                     /locus_tag="JD016_09325"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAU4999834.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALNLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            7829..8659
                     /locus_tag="JD016_09330"
     CDS             7829..8659
                     /locus_tag="JD016_09330"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAU4999835.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            8663..9544
                     /locus_tag="JD016_09335"
     CDS             8663..9544
                     /locus_tag="JD016_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAU4999836.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            9574..10191
                     /locus_tag="JD016_09340"
     CDS             9574..10191
                     /locus_tag="JD016_09340"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAU4999837.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            10194..10460
                     /locus_tag="JD016_09345"
     CDS             10194..10460
                     /locus_tag="JD016_09345"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAU4999838.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            10465..11664
                     /gene="coaBC"
                     /locus_tag="JD016_09350"
     CDS             10465..11664
                     /gene="coaBC"
                     /locus_tag="JD016_09350"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAU4999839.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            11939..14428
                     /gene="priA"
                     /locus_tag="JD016_09355"
     CDS             11939..14428
                     /gene="priA"
                     /locus_tag="JD016_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAU4999840.1"
                     /translation="MKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVIKIGHRILVPFGK
                     GNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWMKNRYLCTYIECI
                     NLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVEKVIDSKGKIKVD
                     KLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESDKIDDYIEQNKIN
                     VGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLEFKDYYREPKSIY
                     KSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVYMEIIEFALNQGL
                     DSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAVKAGKVRVLIGAR
                     SALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNITLILGSATPSIE
                     EYYRAKSGEYKLINIKSRANDKPLPDIEVVDMKDELDKGNRSIFSLKLQKEIHYAIEE
                     NNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRCHYCGYEKEIPKE
                     CPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGALDEILNKFKDKEA
                     DVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLITQVSGRAGRADKE
                     GKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNMLSVVVSGEDERLV
                     IKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQILFKDENIEINLLK
                     GIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            14446..14886
                     /gene="def"
                     /locus_tag="JD016_09360"
     CDS             14446..14886
                     /gene="def"
                     /locus_tag="JD016_09360"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAU4999841.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            14901..15830
                     /locus_tag="JD016_09365"
     CDS             14901..15830
                     /locus_tag="JD016_09365"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAU4999842.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            15840..>16157
                     /locus_tag="JD016_09370"
     CDS             15840..>16157
                     /locus_tag="JD016_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HAU4999843.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIK"
BASE COUNT         6444 a         1627 c         2910 g         5176 t
ORIGIN      
        1 gaaatataaa tatataattt gatttgatag attaaaaaat tatctttaaa cgatacagag
       61 atatcgtaag acaattgcta aattgcactg tatatttttt atataagagc ttcttgtctt
      121 gtgatgagaa gtttttttgt tgtttaaatt gaaattttaa ataaaaataa atttataaaa
      181 aattaaaaga aaggagtaga tttgtcaaat agatatatta tatctttgac aataattatg
      241 atatggtaga aaaagctcaa ttaatggatg aaaaggcaat agcaagagca attactagaa
      301 taagtcatga aataattgaa agaaataaag gtgtagaaaa tctagtttta gttggaataa
      361 agactagagg tgtgccaatt gcaaatagaa tttctaagaa aatagaacaa atagaaggaa
      421 caaaagtaga tacaggagat attgatataa ctttgtatag agatgatttg gaaaaaattc
      481 atgttgaacc tgtagttaaa ggaacttatt tagattttaa tgtaaatgat aagacagtag
      541 ttttagttga tgatgtttta tacactggaa gaactgtgag agcttcacta gatgctatta
      601 tagatatagg aagacctaaa tcaatacaat tagcagtgct agtggacaga gggcatagag
      661 agttgcctat aagagcagat tatgtaggaa aaaatgtacc tacttctaga catgaaatta
      721 tatctgtcag tttattagaa attgatggag aagattcagt tacaatcaaa gaataattat
      781 ttttaagtat tatataaaag cttagcattg aatagtcagg gaggaggata aagcttacat
      841 agattgcaca aaataaatat tataatttta tggatgtgag tcttaatgta gtttttattc
      901 tcctttattt gagtttttga tgtcgtaaaa tgtaaaataa tgataaatta tataataagt
      961 atatagaggt taaaaaatag aatctaggga atgataatta attaaaagat attaattaaa
     1021 cagaggattt atttatatat aataattttg gaggttttta tgaaaaaaac gataatgtct
     1081 ttacaacatc tattagccat gtttggagcg acagtattag ttcccatttt gacaggtttt
     1141 aatccgtcag ttgcaatttt ctgtgctggt gttggtacat tgatatttca tttttgtaca
     1201 gaaggaaaag tacctgcctt tttaggttca agttttgcgt ttataccagt tatactagca
     1261 gctaaagagg catatggtgg agatttagca tatgctcaag gtggtataat agtagcagga
     1321 ttaatttata ttataatgtc gattatagta aaagtagttg gtgtaaataa aattaaatta
     1381 tattttcctg cacaagttac aggagctatg atagtagtta taggattaaa tttattgcca
     1441 acagctttta gtatggcttc agccaatttt gtgatagcat ttataacttt agcaatagca
     1501 attttaacca ataagtttgg tagaggattt ataaaacaat taggaatatt aatagcagtt
     1561 ttttctgggt atataatctg tttgatatta agattagtag atataactac tattacagaa
     1621 gcaagtttat ttgcaatacc taactttaca gttcctaagt tcagtttagg agcaattgtg
     1681 attatatctc cagttgtatt agctgtattt atagaacata taggagatat gactactaat
     1741 ggtgctgtag taggtaaaaa ctttatagaa aatcccggat taaatagaac attacttggg
     1801 gatggatttg cgactatagt tgcagggtgt ttaggaggac cagcaaatac aacttatgga
     1861 gaaaatacag cagtacttgc tataactaaa aactatgacc cttcaatact tagacgtaca
     1921 gcaatatttg ctatattgct tgcatgtgta ggcaagtttg gtggatttct tcaaagtata
     1981 ccaggttcag ttatgggtgg aattagtata atgctatttt cgatgataac ttatgtagga
     2041 ttaaaaacta taagggacag ttcttgtgta gaaagtaaaa taaatatatt aatcatagca
     2101 gttatattat taataggtct tggaacaaca tatttatcta ataaaggtat ttctgttgga
     2161 atccctataa caagtacagt aaagataact ggtcttagtt tagctgcaat tgttggaatt
     2221 gtattaaaca gaattttaaa taatcaggac tttaaagttg aagaagaata aatttaaaga
     2281 ataagttgta tattgaataa ctaaaattag ttaattttct atagacattt gtaattaatg
     2341 ttgatataat gattaaaaat aaatgaaaga aagggcaagg taactaggac aatgagaata
     2401 taatctattt tttataattt aaagtattgt aatttttgaa aagcctattt tggtggcttt
     2461 atttgcaatg cttaaattaa ggagagtaga ttatagtcta tacgtttatt tgttatgcct
     2521 atttatagaa tctctcctta tatttaagtg tgttaaggag ggatttttta tgttgttagt
     2581 aaaagtagag aatttaaaga aatattatgc agataaatta attttggata tagataaact
     2641 tgaaatatta gaaaatgaca aaataggtct agttggttca aatggtcaag gaaaaacaac
     2701 attattaaaa gcaatattgg gagaaataga aattgatgaa ggatatactt accttacaga
     2761 aagctattct tatataagtc agagtgaaaa taatattgaa acatgtggtc atagtaagga
     2821 gaagagtctt ttaaatgctc cagataaatt tgaagaacac ttatcaggag gggaaaaggt
     2881 taagttaaaa atagcagatg cgctgagtaa taagaaaaat atcataatag ctgatgagcc
     2941 aacttcaaac ttagataaaa aaagcattgg ggttttggaa gatatgttta aaaggcatga
     3001 gggagcatta ttattaatat ctcatgatag acgttttctg gatgagttat gtacaactat
     3061 attagaacta gaagatggaa aactaaaagc ttacaaaggc aattacactg attatttaat
     3121 gcaaaaagat gaagaagtaa aaagagctga ttttgagtat caagaatatg ttaaagagaa
     3181 aaaaaggctt gaaaaagctc ttttatataa aaaagcttta agtgatggca taagaaaaac
     3241 accaaaaaga atgggtaatt cagaagctag gttgcacaaa atgggtggtc agactaataa
     3301 gaaaaagttg gactcaaatg tgaaggctat aaaaagcaga attgataaac ttgaggcaaa
     3361 gaataaacct aaagtttcta aagaaatgaa cattaagatt caagatggta tggatataat
     3421 tagcaaaaat ctagtagagg taaaagatat gactctaaag ttagaaaata agctcctgtt
     3481 agataatgtt tcctttaaga taaaaagagg taaaaagata gcattattag gtgataatgg
     3541 gtgtggaaaa agtactctga taaaagaaat attggctgat aaaaatgaca atataaaaat
     3601 aaataacaag gtaaaagtag gttattttga ccaaaatcaa agtttattag atgaggaaaa
     3661 gagtgttcta tacaatacta aagttaatag ttcatttgat gaatctttta taaggataaa
     3721 cttaagccta tttgggttta aaggtgatga tgtctacaaa aaagtaaaag tacttagtgg
     3781 tggagaaaag gtaaaaatag cactatgtaa aataatattg gaagataata attttttagt
     3841 atttgatgaa ccaacaaatt acttagatat aaaatctatg gaagcactgg aaaaggcact
     3901 gataaatact gataaaacta tgcttatagt gtctcatgat agagtatttg tatcacatat
     3961 ttgtaactat attatagaga taaaagatgc taaaataagg gagtttgatt gtaattatga
     4021 cgaatataca attagcagaa ataaaaaaac tcctagtaga gaaaaccaaa tcaaaaaaga
     4081 aaatcttcta gtattagaaa atagacttac aactgttata tctatgttat ctatagaaaa
     4141 agataatcta aaaaaagaat tgtatgaatc agaatataat gaattactaa aacaaatcaa
     4201 taaattaaaa aatagctttt agtgttaact ttttaattac atctatataa gaagactgaa
     4261 aatatttgtt gggaatataa gtatagttat taaaacagaa aagtattttg ttagaaaatt
     4321 aaaagtaagc taattagaaa gtggccttat tattgtactt tataagaagg ccactatttt
     4381 tatttagaat gataatgtgt agttgtctaa catttatttt gtatttagtg cagaatttat
     4441 tcagatttaa ccttaagctt ggctactgtt tcttcttcag gagtaacata aatagtactg
     4501 ttagtttcat aaataaccat acctggttta gaaccactag gttttttcac attttttctc
     4561 ttagtatagt ctacaggcac ctgagaagaa agttttgact tactaaagaa tgctgctagc
     4621 atagctcctt caaatacagt attatcaggg acttccttac cagcacactt tattattaca
     4681 tgagaaccag gaatattctt tgtatgcatc cataagtcat cattatcagc aagtcttagt
     4741 gtaagatagt cattttgctt attgttttta ccaaccaata ttttaaatcc atcagaagac
     4801 acaaattcat gaggttttgt agatgggata gtatcctttt tactgttttt cttttgagtc
     4861 ttgatgtatc caaccttagc aagttcttct tttatatctt gtaattcagc taaattttca
     4921 cagttttcaa tacttaaaat tatgttttct aagtaatcta tttcttcctt atttaaggaa
     4981 atttgatgag atatctcgac tttagcatgt ttcattttgt tatacttttt aaagtatttt
     5041 tgagcatttt cagatggtgt caagttttta tttaattcta ttgttacatc attacaattt
     5101 tcatcataga agttagcaac ttcaatactt tccatacctt tttctaccat ataaatatat
     5161 gaagtaatca attctccttt tatcttataa atgtctgcat tttcggattc tgataattct
     5221 tcttcttgtt ttttaagttt attataaagt ctatcaagct taacagatat actcttcttt
     5281 aaatcagatg aacgttgatt aattctatct ttaatatctt tagttctata gaaatcttcc
     5341 agaatacgag acatactatc cttatttata tatgataaat cgctaaataa tgtaaggttt
     5401 atacaactga aatcaactac cttatccacc tttttatcta taataatgca aggagagtat
     5461 ttgttagaat ttatatcatt gaataaatta caaaatactt tgtgtaaagc atcaaattgt
     5521 tcatcagata tatcctttat gatagcattt tgatttacac ctgctctgta gcaaatttct
     5581 ttagctataa tagggcttat acctagaaat ttagaatata tagacttaaa gataggtcct
     5641 tccaattcct ctaggttttt aataaacaaa tccttagata tattatcaag agggttgagt
     5701 ttgttttgtt ctgggggtaa tgaataatca tgaccaggta aaacttgacg aactctactt
     5761 atgctaaaag ggactctttt tatagaatct ataattttat tttcttcacc atgagttatt
     5821 attatgttgc tatgtcttcc cataatttca atcataatat ttttaactgt cttttctttt
     5881 aattcatcta aagattcaac acttattttt attattcttt caaaacctat ttgagaaatt
     5941 tctactatat ttccaccttg tatgtatttt ctaagtaaca tacaaaacat aggcgctttt
     6001 agagggtttt ctttttgata attacttgta agataaactc taggatttga ggcacttgca
     6061 cttaaaacca atctaaaatt ttctttatta tttcttatat taaaaattac ctcatcatct
     6121 tcaggttgat gtatcttatc tatttttcca cctgtaagtt ttgatgataa ttcatcgact
     6181 attgaatgta taactaatcc atctaatgcc atttatagca cccctttctt tgaccaaaat
     6241 gagtcagtaa attaattata cacataatat acgctttgtg actaggggga atttaaacaa
     6301 tagagattaa tatttaaatt gtatgattat ttatatttat tagagaggaa actttatttt
     6361 taaaattaat aatttaaagt agattaaata taaagtaaat atagtataat tattgtgtag
     6421 agtataagct atgaaagtat atgaacttta cagaaaaaat caattttaag gttctatagt
     6481 atataaatat gaaagaaggt tgaaaaaatg agatttatcg atttaagaag tgatactgtt
     6541 actatgccta ctgacgaaat gagagaagct atggcaaagg ctcctgttgg agattcaatc
     6601 tttagagatg accctactgt aaataaactt gaagaattag cagcagctaa agttggaaaa
     6661 gaagatgcta ttttccttcc aagtggaact tttgggaatc aattagctct atttactcat
     6721 tgcctaagag ggcaagaaat aattattggt aaaggttatc atattgtgac tcatgaggtt
     6781 ggggcacctg ctgtaatagc tggagttcag cttagaacta tagatgaaga cgaaagtgga
     6841 gctctaaatc ctgaacttgt tgaaaaggca attagaagag atgacataca tgaaccacag
     6901 acagggctta tatgtgtaga aaatgcatat tcaggtggaa cagttgtgag tcttgataat
     6961 ttaagagaaa ttaaaaaaat agctgagaaa cataacttac cagtacattt agatggagca
     7021 agattattta acgcagcatt aaatttgggt gtagaagcta aagaaatagc aaagtgttgt
     7081 gatagtgtta tgttctgtgt gtcaaaaggt ttagctgcac caatgggttc tatacttgct
     7141 ggaagtaaag aatttatagc aaaagctaga agaaagcaaa aagttatggg tgggggaatg
     7201 agacaagtag gtatagttgc agcagctggt ataattgcca ttgaaaaaat gactttaaga
     7261 ttgaatgaag accatgaaaa tgcaaaatat ttagcttgtg aattaaataa aattgatggc
     7321 attgaagtaa ataatgttaa tccagatata agtatggtat tttttaagat gagtgaagat
     7381 attataaaag aggatgtttt aataaaagaa ttttttgaaa gaaatataaa aattaataaa
     7441 atggaaaatg gtgaatatag atttgtaact catgtagata ttactaagga agatttagac
     7501 tatgttttaa aaacacttaa agaattaata ggtgtatgtt aatagatatc tactagaagg
     7561 attatagcta gttaccagat gtttaattaa aaaattcata gacagtttaa ataaaacata
     7621 cataaaattc atatagaaat attaatatat aaacatagaa agaactagct taaaaacata
     7681 atataattac cctaattgta atggcaactc tccctcacat cctcaaatta tagttgtcat
     7741 tacaaaactt ttcccataaa ttataagttt atgggaattt ttttatgtta taatagttta
     7801 agcgttaatt tacgaaggag cgatacttat gaaattttgg aaactacatg gaataggaaa
     7861 tgattttata gcaatagatg gaagattcga ccagataaat ccatctgatt atactgcttt
     7921 agcaaaaaaa gtatgccata gaagattttc tgtaggagcg gatggattgt tagttgtgaa
     7981 agattcaaaa gttgcagatg tagaaatggt atattataat tctgatggtt caagagcagc
     8041 tatgtgtgga aatggcctta gatgtttttg taaatttgtt tatgataatt gtatagttaa
     8101 aaaagaaagt tttacagtag atacattaga tggtataaaa gatataaaag taaatcttac
     8161 caatagagaa ataaactcta taagagtcaa tatgggaaaa ggaagtttta tagctaagga
     8221 cgttcctgta cttacaaata aagaacgttt tgtgcaagaa agtataaagg ttttagatag
     8281 ggagcttaaa gttacttcta tgttgatggg ggttcctcat acagtagtta tagtagatga
     8341 attagatgta gatgatgttt gtaaatatgg aaaagagatt gaaaataata aaatatttcc
     8401 tgaaaaaacg aatgtaaatt ttgtaaaggt agaagataag tataatatac atgtttatac
     8461 ttgggaaaga ggttgtggat atactttagg ttgtggtact ggtatgactg catcagcaat
     8521 agtgtgcaat ttacttaata tggtagaaag tagtgtaaat gtaacgtctc aaggtggaac
     8581 agttaaaata gatgtaggag atttctctta tatgacaggt ccagcagtaa aaatatgtga
     8641 aggtattttg gaggtttaat taatggctat aagtatgact ggttttggaa gaggagaata
     8701 caaggatgat aattattact ttttagtaga gtgtaaaacg ataaatcata agtattcaga
     8761 tataaacata cgtcttccaa gaaaaatttc atttttagag gataaagtta gaaatttagt
     8821 aaaaaattat gtaaaaagag gaagagtaga tttatacata aagtttgact tattaggcaa
     8881 agaagatgta aacttaaatt ttgatgaggg attagcatct cagtatatag acatactaaa
     8941 agaaataaaa aataaatttg atattataga tgatattagc gttatgaatg ttgcaaagtt
     9001 tcctgatatt gtgaaaatag aagaaaagga agaagatgaa gatttattgt ggtcaatgtt
     9061 gaatcaagca gttgaagatg cattaatcaa attaagagaa atgagaagtg aagaaggtaa
     9121 aaaattagct gaagatattg ccatgagatg tgatttatta aaaaatcata tagaagagat
     9181 tgaaaaatat tcaagtagtg ttgtagaaga ttatagagaa aaattgaatt tgagaataag
     9241 tgaattactg gatgacccaa gtataataga tgagaataga ttagcacaag aagttgcaat
     9301 atatgcagat aaaagcagta taacagaaga aatagttaga tttaaaagtc atatagggca
     9361 acttaaaaat actatattta aagacgactc tataggtaga aaaatagatt tcttaataca
     9421 agagatgaat agagaaacta acactatagg ttcgaagtca tctgatataa atataactaa
     9481 tttagtagta gaagtaaaaa gcgaattgga aaaaataaga gaacaaatac aaaatataga
     9541 gtaagaatac aatattagaa ggggaatgct taaatgtttg taaaaaaagg attattgcta
     9601 gttgtatcag gaccatcagg tgcaggaaaa ggtactattt gcaaagaact acttaaagaa
     9661 aatgatacaa taaaactttc tgtatcagct actacaagaa aacccagaac tggagaagtt
     9721 gatggagtaa attatttctt cataagtaag gaaaagtttg aagagatgat agaaaaaggt
     9781 gagtttttgg agtatgctca aatttatgat aatttttatg gaactccaaa agcggcaata
     9841 atggaatgtt tagaaaaagg acaagatgta ttactggaga ttgaaatgca aggagcaaaa
     9901 caaataaaag aagtttgccc agagggagta tttatatttg tactaccacc atcacttaaa
     9961 gaattaaaaa atagaattgt tggaagaggt acagaaacag aagctgaaat agagaagaga
    10021 tttagctgtg catatgaaga aattaaaatg attaaggatt atgattattt tatatttaat
    10081 gaagatgtaa aaacatctgc taaagaaata gaaggtataa tatcatctga aaagaataaa
    10141 gttagcagat ataaaaatat tataatagag aaatttaagg aggaattata attatgttaa
    10201 aaccatctat caatgaggta ctagaaaaaa tagacaacag atactattta gtagggactg
    10261 tttcaaagag agcaagaaaa ttaattgatg gagaagaacc atatgtttca aataaaacta
    10321 aggaaaagcc tgtatgtgta gcaactaaag aagttgcaag tggaaaaata acatatagat
    10381 tattgacaga agaggaaata gagatagaag aagctagaca tcatgctgag caacaccaac
    10441 aaatttcaga agaggagtaa ggtcatgcta aaagataaaa ctgttgtaat tggcataagt
    10501 ggcggtatag ctgcatataa ggcttgtgat gttgtaagta agttaaaaaa actaaatgca
    10561 aatatccatg ttataatgac aaaatcagca acagaatttg ttaaaccact tacatttcaa
    10621 tcgctgagtc aaaattatgt agtagaagat atgtttgaag aaccaaaaac ttgggaagtt
    10681 gaacatatat cattagcaaa aaaagcagat ttattcttaa tagtgccagc aagtgcaaat
    10741 gttataggca agattgcaaa tggtatagct gacgatatgt tgacaactac tgttatggca
    10801 acaaaagcac aggttcttat agcaccagct atgaatacta atatgtatga aaatccaatt
    10861 gttcaaaaaa atatacaaac tttaaagagc tttggatata aatttattga accggaaagt
    10921 ggaaggcttg catgtggaga tattggaaca ggtaaactag ctaatccaga tataatagta
    10981 gaggaagttt tgactaatct tactaaagag caagatttaa aaggtcaaag tatgatagtt
    11041 acagctggtc caactgtaga gagtatagac ccagtaagat atttaactaa taggtcttct
    11101 ggtaaaatgg gatattctat agcaagagaa gctatagcta gaggtgctga tgtaacatta
    11161 atatcaggtc ctacaaatct ttctattcca caaaatctta aaaaatttgt tcaaatagag
    11221 tcagcacaag atttgtatga agcagtagta tcaaatttaa gtgaaaataa aatagtaatt
    11281 caaagtgcag cagttgcaga ttataaacca aaaacttatt ctgacaaaaa aattaaaaag
    11341 aagaatgatg atttatcaat agaactaagt agaaattatg atattgctta tgaaatagga
    11401 aaaataaaag aagataagat actaattgga tttgcagctg aaactaatga ccttatagaa
    11461 catgccaaag gaaaaataaa aaagaaaaat ttagatttta ttgtggcaaa tgatttaact
    11521 aaagaaggtg ctggttttag agctgatact aatattgtaa aaataataga tgtagaggga
    11581 aatattactg agtatcctaa gatgaaaaaa gaagaagtag ccaatgttat tttagataaa
    11641 gttaaagcgt tgcttgagaa ataagacctt ttttaaatat gatatttgtg tattttatca
    11701 cagctaaagt ggctgaaagc tgcaatttaa ttttagcgag taaaaactcg caaatgtcag
    11761 ttaaattgta gttttcagca tttattttgt cataaagtat aaaatataat taatagttaa
    11821 ttattaaaat aaagttaata ttaataactt gaaattgctt ataaattctg agattttaat
    11881 atcttgtaaa gtatattaaa atatgaacaa tatattaaat ttaagtagag gttttataat
    11941 gaaaaaatat gcaaaggtaa tagttagaag taatactata tatacagata atttatttac
    12001 atatcaaatt cctgtatttt tatctgatgt aataaaaatt gggcacagaa tattagtacc
    12061 atttggaaaa ggtaataaac caacagaagc ctttgtattt caatttacag atagcttaga
    12121 tgaaaaaata aaaataaaag aaattattga tatattagat gaaaatccga tatttagaaa
    12181 agaagattta gagttggttt attggatgaa aaatagatat ttgtgtactt atatagaatg
    12241 tataaatttg atatatccaa agggatacaa attaaataat tataaagtag tcctattagg
    12301 tgaaagttta agtggattaa atgatattga attgaaggaa aaaatatcaa ctttaagtga
    12361 taaaaataga gaaatagtag agaaagtgat tgatagtaaa ggtaaaatta aggttgataa
    12421 gttaaaatat ataccaaact taaatagttc tttgtacaca atgaataaaa atggaattat
    12481 aaaattatgt tgggaatata aaaatcacaa aaatgaaaag aaggtatgtt atatatcttt
    12541 gagtttagaa agtgataaga ttgacgatta tatagagcaa aataaaataa atgtgggaag
    12601 taaacaaaaa gagatattaa gttttcttaa aaataatgaa aatgtagaga taaatgattt
    12661 attggattta ttaaatgctt caaaacaaag tataaattct ctaagtaaga aaaagctaat
    12721 aaccttagaa tttaaggatt attatagaga gccaaaaagt atttacaaat ccgtactaaa
    12781 aagtataaaa ttaaacaatg agcaacaaga agcagttgat gagataaagt cgaatatgtt
    12841 tgtagatgat aaaaaaccat acttaattca tggagtaact ggaagtggaa aaactgaagt
    12901 ttacatggaa ataatagaat ttgctctaaa tcaaggctta gacagtatat tcttagtacc
    12961 tgagatagca ttaactcctc aaacaataga taggctaaag agtaggtttg gagatttggt
    13021 tggagtgttc cacagtaaac tatctgaagg agaaaaacat gatgtttaca aagctgtcaa
    13081 agcaggtaaa gtaagagtgt taattggagc taggtcagct ttatttgcac cttttaattc
    13141 cttaggctta ataataattg atgaatgtca tgagtcatca tataagtctg agaagaatcc
    13201 aaaatttaat gctatagaag ttgctagatt tatggcatta aaaaataata taactcttat
    13261 tttaggttca gcaacgccct caattgaaga gtattatagg gcaaaatctg gagaatacaa
    13321 acttataaat ataaaaagta gagcaaatga taagcctctt ccagacattg aagttgtaga
    13381 catgaaagat gagttagata aaggaaatag aagtatattt agtttgaaat tacaaaaaga
    13441 aatacattat gcgattgaag aaaataatca agtaatacta tttttaaaca gaagaggtta
    13501 tgcaaatttt gtatcttgta gaaagtgtgg atatgtgttt caatgtgaaa attgtgatat
    13561 atctttaact tatcataaga aaagcaatac tggaagatgc cactattgtg gatatgagaa
    13621 agaaattcca aaagagtgtc ctgaatgtaa aagtacttat gttaagccat ttggagttgg
    13681 aactcaaaaa attgaagagg aattaaaata tatttttcca gatataaaaa ctttgagaat
    13741 ggataaagat acaacttcaa aaaaaggtgc tttagatgag atactaaata aatttaaaga
    13801 taaagaagca gatgtactta ttggaacaca gatgttaagt aaagggctgg attttgagaa
    13861 tgtaacttta gtaggtattt tgtctgcaga catgatatta aattttccag actttaaaag
    13921 ttttgaaact acatttcaat tgataactca agtctctgga agagcaggga gagctgataa
    13981 agagggaaaa gtagtacttc agacttatga tactgaacac tatgctatta agcatgctat
    14041 tgaatatgat tatgagggtt tttatgaaga tgagataaaa ataagaaaag catttggata
    14101 ttctcctttt aataatatgt taagtgtagt tgttagtggt gaagatgaaa gattagtgat
    14161 aaaaaatata aaaaatatgc atgcatcatt gatttattta ttagaaaaaa ggggtataaa
    14221 tgatttagga tttatattag gtccaaatcc atgttcaata tcaaaaataa atcaaaatta
    14281 tagatggcaa atactcttta aagatgaaaa tattgaaatt aatctcttaa agggtataat
    14341 aaaatatata tgtataacaa aacgagattt gatatttgat aagaatatca atgtatctat
    14401 agatattaat ccaaacagtg tattataaat ttaggaggaa ttataatggc tttaagacaa
    14461 atagttcaaa taggagaacc agtattaaga aaaaaatcaa aaaaggttga aaaaattgat
    14521 gaaaagataa tacaattatt agatgatatg gcagaaacta tgtatgatgc agatggtgtt
    14581 ggactggctg cacctcaagt tggaatatta aaaagagtag tagtaataga tattggagaa
    14641 gagcttatag aacttataaa tcctgaaata atagaaacat ctggggagca aatagatgag
    14701 gaaggatgtt taagtgttgt tggagaagcg ggaaatgtta gaagaccaaa ttatgtaaag
    14761 gtcagagctt taaatagaaa tggtgaaaca atagagttag agggagaaga acttttagct
    14821 agagcatttt gtcatgagat agaccactta gatggaattt tatttgttga caaaatagaa
    14881 aagtagtagg agttgatttg atgaaaatag tatttatggg aactcctgat atagcagttc
    14941 catgtttaca aaaaataata gatgagaagt atgaaatatt gggtgttgta actcaaccag
    15001 ataaaccaaa aggaagaggt aaaaaactag gtatgagtcc agtaaaagaa ctggcaattg
    15061 aaaataatat acctgtatat caaccagtaa aggctagaga taaagaattt atagataaaa
    15121 ttaaatcttt aaatccagat gtaatagtag ttgtagcttt tggacagata cttccaaaag
    15181 aaatattaga gattcctaaa cttggatgta taaatgttca tgtttcttta cttccaaaat
    15241 atagaggtgc ggcacctata aattgggtaa taataaatgg tgaagaaaag actggtgtta
    15301 caactatgta tatggatgaa ggattagata ctggagatat gatactaaag acagaagtaa
    15361 atcttgatga aaatataaca gcaggagaac ttcatgataa aatgatgaat ataggagcag
    15421 aaactttaaa agaaacatta agattaattg aagaaggaaa tgctccaaga gaagtacaaa
    15481 atcatgaaga attttcctat gcgccaataa tgaataaatc attaggtaac atagattttt
    15541 caaaaagtgc cagagaaata cacaatttag ttagaggagt aaatccatgg ccaagtgctt
    15601 atactaccta taatgatgtc ataatgaaag tttggaaaac taaagtgtta gatgaaaaaa
    15661 gcacaaaaga tgtaggtaca ataattgatg taagcaaaga tgggataaaa gtaagtacta
    15721 tagataatgt acttttaatt gaagaaattc agatgccgaa taaaaagaga atgttggttg
    15781 gtgaatatat aaaagggaat actatagaaa ctggtttagt attgggttag gtgaaaagta
    15841 tgacagatat aaagaagtat ttaacaagtg ttgggatttt gattttaata ttaggaattt
    15901 tgataggagt tgttaatttt tttataataa gtttaactca aatattctcc ttgatgataa
    15961 atattataat aatatcacta atacttataa tactatgttc aacagcagtt acttatagag
    16021 taattaaagg aaagtatgtt aactcaaata taatgaaaat aaattttaat attgtcagtg
    16081 gattatttcc tattatatct ttcattgcat catcatttgg tatgtcaaaa agtgatatta
    16141 gaagaatata tataaag
//
LOCUS       DACXMZ010000074        16145 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.074, whole genome shotgun sequence.
ACCESSION   DACXMZ010000074 DACXMZ010000000
VERSION     DACXMZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16145
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.074"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(85..2151)
                     /locus_tag="JD016_09375"
     CDS             complement(85..2151)
                     /locus_tag="JD016_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAU4999844.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQNEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(2228..5263)
                     /locus_tag="JD016_09380"
     CDS             complement(2228..5263)
                     /locus_tag="JD016_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAU4999845.1"
                     /translation="MSMENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKK
                     LNDNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGN
                     NEEINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDL
                     DPKFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAA
                     IDNPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELL
                     PYSEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYK
                     IKNKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIM
                     SIAISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIE
                     LLSANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYM
                     EKNKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWVRLNIFEDELKILGVVVDV
                     TKEFIEKKKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYI
                     NDTYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSV
                     KKQMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVS
                     EFDINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFS
                     SPIDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFE
                     EIEDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLL
                     YIMPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMKTLINL
                     GVDYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(5310..6770)
                     /locus_tag="JD016_09385"
     CDS             complement(5310..6770)
                     /locus_tag="JD016_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAU4999846.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(6862..7236)
                     /locus_tag="JD016_09390"
     CDS             complement(6862..7236)
                     /locus_tag="JD016_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999847.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKVNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(7412..8158)
                     /locus_tag="JD016_09395"
     CDS             complement(7412..8158)
                     /locus_tag="JD016_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAU4999848.1"
                     /translation="MFMLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAE
                     KYKQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKVGEEIQKYLN
                     ENGMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRV
                     EGHTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENS
                     SEEGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(8173..8961)
                     /locus_tag="JD016_09400"
     CDS             complement(8173..8961)
                     /locus_tag="JD016_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HAU4999849.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEVATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKTINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(9049..9139)
                     /locus_tag="JD016_09405"
     tRNA            complement(9049..9139)
                     /locus_tag="JD016_09405"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9103..9105),aa:Ser,seq:gct)
     gene            9398..10162
                     /locus_tag="JD016_09410"
     CDS             9398..10162
                     /locus_tag="JD016_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAU4999850.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(10443..11558)
                     /locus_tag="JD016_09415"
     CDS             complement(10443..11558)
                     /locus_tag="JD016_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HAU4999851.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(11599..13125)
                     /gene="glpK"
                     /locus_tag="JD016_09420"
     CDS             complement(11599..13125)
                     /gene="glpK"
                     /locus_tag="JD016_09420"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAU4999852.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGIPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTNQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            13418..14515
                     /locus_tag="JD016_09425"
     CDS             13418..14515
                     /locus_tag="JD016_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HAU4999853.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(15303..15815)
                     /locus_tag="JD016_09430"
     CDS             complement(15303..15815)
                     /locus_tag="JD016_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999854.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
BASE COUNT         5204 a         2827 c         1691 g         6423 t
ORIGIN      
        1 ttaaataata cactttacac atatctattt gtcttgcaca taataaataa tacattatat
       61 ttctatctaa atttagattc taaattaaat agtataaatt atattcttca ataaacaagt
      121 tttcaaatcc aggattcgct ctctatcttt aaactttatt gtaaacataa ttccatcttt
      181 tttaattatt tctccttcat gaaatttttt atggtatacc ttatctccta caccaatact
      241 gtcaatttct tttttcgttg gagattttat atcattaata aataatgatt tatcaacttt
      301 cttaccatac ttatttaaag tagagcttat acataacttt tcttcagctc ttgtaatagc
      361 aacatacata agtcttcttt cttcttctaa ttgttcttct tttttctctt ccttacaaat
      421 atcatatgat ttttcatgag gtattgttcc ttcatttaca cctataatat aaacatttct
      481 aaattctaat ccttttgcac tatgcatagt tgtaaatatg acaccctcat tctgcttatt
      541 cttgttattt tccgttaact cagtctttac tctatcaata tgctctaaaa actctgtaac
      601 agtattaaaa ttagttgctg aactctcaag ttcatttaat atctctacaa gtccattagt
      661 ctttatcttt ctgttgctac aataatctaa aacatatcta tcataatcaa gactagttct
      721 aatataagat attgcatttt taggatttaa agtatttaaa taacttaaat ctatttccaa
      781 atcatttatt gttttaactt gtttaggatg aagattacac ttatttatta atgctgttat
      841 aaagtctttt tcatccttaa ccatatttat actgtctttg gatatatatc taaaaggctt
      901 gtttataatt ctcaaccaat ctttatttga ttttggatta atacctattc ttaaataggc
      961 tagtatatct tgacttgccc aatggtcata tattgtaatt actgagtctt ttactacaaa
     1021 tggaatcctc atatccataa atacatctac taatgctctt gactgtatat tagttctata
     1081 aattactgca aaatctgaat actctacata atctttttgt atttcgtcaa ttatttccct
     1141 ggcaatatat aaggcttctt cctctgaatc atgcggagaa atataggtaa cacttcctcc
     1201 atccccctga ctacacttaa tgattttttc atatctattt ttatttttgt cgataagtct
     1261 atttgcagta tgaactatct cacttttaga cctgtaattt atatctaata ttattttttt
     1321 agtattatta aagtattgtt caaattcaag caaaaaatct ggtctagcac ctctaaatcc
     1381 atatatactt tggtcttcat ctccaactgc aaatatattg tttaatgggc tacaaattaa
     1441 ctttaaaact tcaaactgta ctttatttat atcttgaaat tcatctatca atatatattt
     1501 atacacattt ctaaccattt ctagtgcaga cttattgttc aaaagtaaat aataagttct
     1561 tataagcata tcatcaaaat ctattttatt aactttagat ttttgttctt cgtataagtt
     1621 gtatactttt aaaaactcat cttttgttaa tacttctgaa ttaaattcat ttttatccat
     1681 aagttcattt tttacatagg aaatttcatt tataacttgt cctatattct catcatcatc
     1741 agcattctct atatttaaac tttttaggat tgccttgatt gtcattcgct ttgacttttc
     1801 atcaaatata ctgtctaagt tgtatctttc aaagtatctt agaattttaa aaaatacaga
     1861 gtgaaaagta ccataggtta ccttattaag tcttatatca tcacttaaat tcaaagctct
     1921 atttttcatt tctattgaag atgccttggt aaaacttatt gccaagattc ttgttggtgc
     1981 tatgtttttg tttacaacca tattggctat tctatatgta attactctgg tcttacctga
     2041 accaggccct gcaagtatca tgcaaggccc atctatatgc tcaactgctt ctaattgatt
     2101 ttcatttaac tttcttatat ctatatttgt tgtgattatt agctccacca ccttgattta
     2161 taattatatt attttctttt taattataaa ctaaaagttc ataaaaataa atttaatttt
     2221 tcctctatta aaccacatca ttcctatttt catgacatat cttgagttct tttttaattt
     2281 tgtcgcttat atcttcaggt ataaatgatg gtttactaat atagtagcct tgcatataat
     2341 ctactccaag attaattagt gttttcattt caccttttgt ttctacacct tctgctatca
     2401 ctttaatatt tctttcgtta gaatatgaga taagattttt taatattttc tgtctgtttt
     2461 catctttatc aatacctctt acaatagaca tatcaatctt tacataatct ggcattatat
     2521 ataatagtac agcatctccg ttgtaacctg tcccaaaatc atctaaagca atttctatat
     2581 tccaatggtc tatatatgct ttctttttct caataaaatc ttcattaatc ttatcattct
     2641 ctgttatttc tagtactact ctatttaaat attcaccata taaatcttca atttcctcaa
     2701 aatctacttt tgacaaatta ttattaggaa tagaatttaa gaacagtttg caatcgccaa
     2761 atttttcttt gtatttttta aatgatttaa gtgctttaaa ccatgtaata cgctcaattt
     2821 gatataattt agactgtgaa cgagctaatc taatgacatc tattggcgaa ctaaaagttt
     2881 ttattttagg tctcataagt gcttcatatg caaatacttt acctttacaa tctacaatag
     2941 gctgaaatgc atactcaact aattgaccat caatcaactt gtttaattct tctctattac
     3001 tcaataagaa tgaatcctta ttatatatat taatatcaaa ttcactaact tcacccttga
     3061 ttgtattttt aatttgatac atagcaaaat ctgcgtatct cattaattca tcatagttgg
     3121 ttgaatcatc agggtaccag gaaattcctg ctgatgccct tattttaaat tctgttccat
     3181 catgaagttt taataaggta ttattcatct gtttcttaac agaattaata atatctctta
     3241 tactatcttt actttcatat ccatatataa atacataaaa ctcatcacct gaaagtctag
     3301 atactatacc attccatgat ataaacttct taagtatgtc tgcagcacac ctaatatatt
     3361 catcaccagc atcatgtcca tatgtatcat taatatattt tagattatct aaatcccaca
     3421 taataaatgc tgctatctta agttcatctt tattcttaaa tttttcttgt atctgtgaat
     3481 gaaaagctct cctatttaaa agatttgtta aaatatcaaa atctctttca tattctatct
     3541 tctttttctc aataaattct tttgtgacat caacaacaac gccaagaatc tttaattcat
     3601 cttcaaagat attcaatctt acccacttaa gttttttatc tttagattca aacttatata
     3661 tatatgtatt ttctgtttca taactttctt gcaaatattt atctagtccc cttaattttt
     3721 taagaaattt atttttttcc atatatccat attcttcaaa tttgatttca gtagtaaaca
     3781 cgctaaaaaa ttcttttgta caaaaaacat tttctgcgtt tttgtataac tcaaaagccc
     3841 caattggcat atctaccatt tcaataatct gtgataattt tgacgaagaa tctgcaacat
     3901 ttgcactgag caactctata gctgaagata attcatctat ttcagatata tttatcttat
     3961 caagatttac tggttttgat ggattactgt ttctaacttt gttggctaaa agaacaattg
     4021 gttttgtaaa taatcttgat gcaatgtaaa tacctattag acctattaat aaagaaattg
     4081 ctatagacat tataactaat ttttctaccc tttttgaaaa tgacaaaaga cttttttctt
     4141 caataatacc caaaagagcc caacgttcat tttcaaatgg agtattggag ttatataaat
     4201 ctaagtattg tacactagca taaatgttgc cattaccaca ttcattatct ttatttttaa
     4261 tcttatatat atttttatac gattccttct gctctaaatt agaatgtaaa ccatcccaaa
     4321 atttttcttt taatgagtat ccatttgaaa ttacattttc aaaggccata tcattatttc
     4381 tatctacacc taaaatataa ttaccctttt tattcccatt tatctctgag tatggaagta
     4441 attctcttaa ataatttaca cttaaatcta ttccaagtac tccatataca gttccatctt
     4501 cataaataag gggaactgta tatgtcataa gcctatcacc tgcatttgtc aataaaaact
     4561 tacttcccca gtatcctaaa ttagaacttg atacatttgg attatcaatt gcagctctaa
     4621 atggtttata aaaaaaatca tctccctctt gctcattttc acttttaaat gaaaacttag
     4681 gttcccaata actgtccata gatattccaa aacttcttgc tatatctgat gaacctctct
     4741 cgattaacaa atcagaatta tcatttggat taaacttagg gtctaaatcc cttatataca
     4801 atcctgtcct acttatttca cctttactat ctattgactt atcgttaata tttttgtcac
     4861 ttaggattat aaaagctcct gtaacagaat tttttctcaa aagatatatt aaatcatttg
     4921 atatgctttt tataatatct ttgttaattt cttcattgtt gcctacatcc tcaattaagg
     4981 catttttttc atttaatact ttttttacag aggaattaat tgtagcttcc gtttcacttg
     5041 tatttgacca tctttgtaac atttcatttt gtatataatt tttacgatta attactcttt
     5101 catttaagat gtcaaacgaa ttatcattta atttcttaat agttcctccc cataaaattg
     5161 tggcacaaaa taaacttgtt tgaattagca taacaataat taggggaaat atcatctttt
     5221 tcattattga attgtttttt cttagattgt tttccatact cattccacct atacctttgt
     5281 gcttgtcaac acactaactg ccaattcatc tattttttta tagcattttc aagctcagcc
     5341 ttaaaacttt caaaccaagt tttaaaattt ttatcattat taaattgtgc tactgcatcc
     5401 ttcttagaag ttccactttt tactaattca tttattttat ttgaatcttc tttagcttta
     5461 tcaagcattg aatattcaag tatttgtcta gctttagttc caccatcaaa tgctttatta
     5521 gtatataaat tacaactgtt aatctgttca atagatgagg ctattgaacc ttcaacttta
     5581 tcagataaac tcaaattatc tttcttcata acttctttta taacatccaa atcattcgtt
     5641 tttttcttta ctggtaagta tccagaagca atagagaatt ttatattagg ttctttatct
     5701 gtaaaccatt ttaaaaattc tactgatgca tattcttctt ctttagtaga ttttgttaca
     5761 gccattcctg caccttgttg tacagcatat tttccttctc ctttaaaacg tggtgcttga
     5821 aaaacagatg tttcaatatt ataactctca ttatcattta gcattactct atctggaaag
     5881 tatgctgctc ctgaactaga acctacaaga gctaatatat cacctgtttt tgcatcatca
     5941 gaacgaaact taccataaga agcaaaatat ccatttatat atggtacata gaaattatcc
     6001 catagttttt tcataacttt ttcatcaata tttagtgtaa ctttaccatc tttaacagaa
     6061 aaaatttcta ctcctagttg tttacttcct ataataatat aatttgctaa agcatctctt
     6121 ccaaaaaatg ctttaccatc attaggctct ttcgttaaac tatctgtcca ttcataatat
     6181 ttttttgatg tctctactaa tccttcaatt gtttttatat catttatatt tgaatttgta
     6241 gcttttgcaa atttatccca atcagtttta tttaacatca aaatttcagt tgattttgca
     6301 attgggaaaa gtttaaactc atccttagaa tttattctgc cttcttctac atattcgtct
     6361 atgtattcat ttaattcttc tttacttatg tacttatcca tatctactag taaccctaat
     6421 ttatccattt catatgcagt atctgagtat gctgcaaata tatttggtac atcttcagaa
     6481 ccaacttttt tatttgcaga atctaaaact ttctctgata aatcaactac attaccttga
     6541 ctgaatgctt caacaataat tcctttttca gcacctacag tgtcattaaa ttccttaaca
     6601 agcttatcaa aagccatttt ctgctgtcca ttataataat gccaaatttg tattgatgtc
     6661 ggattttctg ggtctaactt ataattatct tccttcttta aactacatcc tgtaagcaat
     6721 aaaacaatca ttgatgtgat aattaatttt gcaaacttac acctattcat aaaatctact
     6781 ccttcaaatt ataaaattta taatagcatt aaaactctaa cttcactatt tatattaatt
     6841 atagaacgtt tattgatata tttaaacagg atttacaaca tatttaaaag caattaagaa
     6901 acatactatt acaaatatag aaaataaagc ttttacataa tttttatttc taaatgcacc
     6961 aaatgacacc aatccttgtt gaatagctac tgtaattact aaaagtatca ttattatatt
     7021 aagttgaacc agtttaaata agtaaagcaa tgaaaacact actagaagta cacataatac
     7081 aatactagat ttattaataa tatcatcaat cttatctacc ttcttagatt gcttattaac
     7141 tttatttaat ttttctttat ttaaattctc aactttttta ttataccttt ctcttgtttt
     7201 atctttatat ttagtatttt tatttttatt tcccatttat tttccatcct ctataaattt
     7261 tttaattaag cctatacatt atttaaatat tatatattta agataccata tattcctaca
     7321 aaaaggtaac ttactatgta tctatttttg attttcgcat aaaaaagcca tctcctttag
     7381 aattaattct gtattgagac agctaattta actatttatc ctttgaaaca ttttctacat
     7441 tatcatattt actatcaact aaaactatat caactcttct attttttgac ctaccttctt
     7501 cagatgaatt ttctgctatt ggtctaaact caccatatcc tacagcagat aatttatctg
     7561 gtgctatacc tgcattgtca ataagaagtt gaacaacatt ggttgctctc ataactgata
     7621 aatcccaatt tgacttaaac tctgaatttt ttatagacat attgtctgta tggccttcta
     7681 ctctcatgta actattcatt tcatttaaca tcttacctat ttgaataatc ttatctctag
     7741 aattatcctt cacaatcgct ttacctgtat caaatagtat tgtatcttta aggcttatta
     7801 ctaatcctct atcttgaact cttaatgata ctgaattagc cataccattt tcgtttaaat
     7861 acttctggat ttcttcacca acttttttaa acttagcatt ttgtaaagta tctaaatcat
     7921 tacttcctgg ttcaactact ggttctattt tgctattacc accttcaata actccagaag
     7981 aaccaccaaa agcagaattt agagattgag ataattgttt atatttctct gcatcaacat
     8041 tactcataga atacattatt acaaagaaaa tcataagtaa tgttatgagg tctgaataag
     8101 ttaaaagcca tcgctcattg ttttcttctt tttcatcttc atcatacaac ataaacattc
     8161 catcctttta tattattcag ctctaccacc aatttcttca agttcaatta gttcttttga
     8221 atttaaaaat atttttaatt tttctgtcat taaattagaa ttatcaccct caagtatgga
     8281 taatattgct tctatcacca aaagtctttc acttacttct tgttgattta tagtcttaag
     8341 ttttgatgct attggtaacc atattaaatt ggcagaacca acaccataca aagtagccaa
     8401 aaatgcaaca gaaatttgag gtcctaatgc atctggatta cttgaaagat tacctaaaat
     8461 atgtactaat cccataactg tacctattat ccccagtgta ggtgcatatc cacctgctga
     8521 atcaaaaatt gctattcctt ctttatgtcg ttttgatgtt atctgtatct gtgattccaa
     8581 tatatttctt atagtaactg catccactcc atctaccata agttcaagcc cttttctaat
     8641 aaaagggtct aattcatcat tctcacttat ttctttgtca atactaagaa gcccctcttt
     8701 cctagccttt gttgcaactt ctttgaaata gattatattt tctatgttat taggcttttt
     8761 atttttaaaa gctaataaca ttatctttat agacttttta aacacaggga atggagtcga
     8821 agctccaaca gctcctatag ttccaccaaa cacaacaagt gctgatgaaa atacaaataa
     8881 tgctccaaca tgtcccccat caataacaaa agcaactacc aaagatgcta tccccactat
     8941 gaaaaaaatt aaagttgtca tctattccct cctttcttta gttatacata tctaatcatt
     9001 tatgacatta taaaaagact atctttaata aaaatagcct ttaataattg gcggagaagg
     9061 agggattcga accctcgcac cggttaccca gcctaatccc ttagcagggg atcctcttga
     9121 gccacttgag tacttctcca tatctctata ctaataaatt atataataaa atcatatttt
     9181 agtcaattgt tttataaaat ttgagttatt ttgaattatt aaacatctat ttttactttt
     9241 ttgcttaaat gtaagtttta aatatggtaa aattatcttg cataacactt ataatattat
     9301 ttgataaata tacatacatt aacaaatata cctaaactct gacttaattg taagttatgc
     9361 caaaactaaa ataatactag tccaaggagg atattttatg ttaccacagg aaagatatga
     9421 gaagatttta agtattctca aatcagataa tataattaaa ataaatgaca tagttcaaat
     9481 gtttgatata tcaatagaaa ctgcacgaag agatttagcc catatggaga gtatcggtct
     9541 tttaaaaaga gtttatggtg gtgctatacc agttatagca agtagcgtag aacttaacta
     9601 tgttaaaaga aaaaaaataa actcaaacga taaaaaacag attgctatta aatgtagtga
     9661 gtttataaat acaaatgata ctatttttat ggatactgga actactgtac ttgaagtttc
     9721 aaaaaatttg aaagataaaa agaatctaac tgtaataacc aactcaattt tagttgcaaa
     9781 cgaacttatg aatactaata taaaagtata tgtgcttggg ggctatctta gagaaactga
     9841 aggttctttt tctggtccgc ttacactgtc agctattgaa caatttaatg ttgataaagc
     9901 tattttagga gctggaggta tcactcttga aaatggaatc tctgactatc acttagaaga
     9961 agcacttgtt agaaaaaaaa tgattgaaag gtctagaaaa tctatagtag ttgtcgaacc
    10021 tcctaagttt ggtataaatt catttgcatc agtagctcct atagaacata tagatactat
    10081 tattacctct gctagtatag accataatat aataatagag ttttctcaaa aaggaatacc
    10141 tttagtcata gctgataatt agcttatatg attgattttt agttccttga tgtaataaat
    10201 taaaattaag gagccaaata attatttatt tagctccatt tatgtctaaa ctatacttta
    10261 tattttaatt atgtaactac tttcagttag cttatcattt ttagttattt gattgccatt
    10321 ttatttaatt tataagtttt agttattcga ttatttttta attgtctgtc tttttaattt
    10381 atttttagtt atttaaactc ctactttaaa ataatttaaa tttgacttaa gtattgttta
    10441 ttctacactg catctttctt cattttaata ctttgttctt cttccaaagt taaatctttt
    10501 gtcataagta tagcaagtgc aattgccaat actcctccaa ccaattttcc aattacaaca
    10561 ggtgtaatca tctctggaac tactcctgct gtaaatccta aatggtcccc caaagctgct
    10621 gtagcacaaa ccagccaagc agtatttact actttcccct ttggactcat atctttcatc
    10681 attttatata ctggaatact attggctaaa gtaaaaacta atcctgctgc actagtggca
    10741 tccataccta attttccacc cactattgta agtggtttat taagaacttt aactaataaa
    10801 tgtaatattg gaaaagtacc taataacacg attgcaatac ttccaattac ttgaatacct
    10861 tctcttatag gtgtcatgcc ttttataagt actatacctg ttatttcttg aaatgctgct
    10921 gctcccaaac ctattgttat caatatagat ataaattttc caaaaactaa tgacccattt
    10981 atcataagcg tagaattaaa ttttaaacct actgccaata ttactgccaa tattatcact
    11041 ggtatcatat ttataattac cattgataag ttaaatcctg ctattaatcc tcctataata
    11101 cctccaaaag gtattgttat caagcctatc agtaaccctt ttgcaaagaa tggtctgtct
    11161 tcatattcta taagacctag tcctactgga attgaaaata ctaatgtaca tccaagcatg
    11221 gatgacacaa ccaatcctgc aagcattcct gcctctttat tgtgtgcaag ttccatagcc
    11281 aatggatacc ctcccatatc attcgctaaa atagttgcaa acatagatgg gtcagctcca
    11341 cagagattga agattggtat tattatagga cctagtacat ctgaaattac tggagctaaa
    11401 caaactatac caaccattcc taaagccaat ggacccattg aattaaaccc ttcttcaaac
    11461 tgctctccaa gtccaaattt atttccaatt attctatcta acgctcctaa aagcactccc
    11521 acagccatta tgtataatat aattttatca aagggcattt ttaattcctc ctatacatgt
    11581 aaaatactct attgtaactt aattagcaca cacctttttg gtatcgtttt cccaattcat
    11641 agctcttttt actgcttgat tccatccaag taatatttct tctctttttt gattagtcat
    11701 atttggaata tattttttgc ttattgtaaa ttcacttcta atttcatcaa tactgttcca
    11761 gacaccacaa gaaagacctg ccatatatcc tactccaagt actgtcgtct caggattttc
    11821 tgcaatttca attggtatac ctaatatgtc ggattgaaat tgcattgtaa agttattttt
    11881 agtaattcca ccatctactc tcaacatttt tatttttttc cctgaggcta agttcataga
    11941 atcaacaaca tctttgactt gaagtgcaac agactcaaga gtcgctctta ctatatgctc
    12001 ttttttagta cttcctgtta tcccaaccat tgtacctcta gcatattggt cccaataagg
    12061 tgctgctata cctgcaaatg ctggtacaaa atatacacct ccagtacttg atatggaatt
    12121 tgccatgtca tcagtttctg aataattttt tataactcca atgccatctc taagccattg
    12181 tattgcagca cctgctatat acactccacc atcataagca tatgtaactt tatctcctat
    12241 tttccaagca acagttgtca aaataccatt acctgctata tttagttcct cacctatatt
    12301 catatatagg aaacatcctg taccataagt atttttaacc attcctggtt caaaacatcc
    12361 attgccaaat aaagctgctg gttggtctac cattgaagca gtaattttta tagaaacccc
    12421 acaaaatgcc tccttatctg tagttccaaa atcaccactc gtaggcatta tatctgctaa
    12481 aatacttcta ggtattccta taatgtcaag caactctcta tcccaatcca tatctcttat
    12541 attaaaaagc attgttcttg ctgctgtaga tacatcagtt aaaaaagatt ttcctcctgt
    12601 cattttccat attaaccaac tatctaatgt tcctgcaagt acttctccat ttttaatttt
    12661 ttcttttaca ccttttacat tatctattac ccatttaatt tttgtacctg agaaatatgg
    12721 gtctataatt aacccagtct tatctattat cttattttct aaacctggta tattctttaa
    12781 attttccaca taatcataag ttctcctgca ctgccacaca atagcattgt aaagtggttt
    12841 acctgtattt ttatcccata acattactgt ttcaccttga ttatctatac caattccttt
    12901 tatatcactt tcattgattc cattttcaaa agcctgttgt aatacattat tactcacagt
    12961 ttttgtatta ttccaaattt ctatagggtc atgttcaaca tatccagatt gtggatatat
    13021 ttgagtatgt tccatataat cgctaaaaac aaactctgat ttttcgttta tcaatactgc
    13081 tcttgttcct gttgttcctt ggtcaattgc taatacatat ttcactttaa atgtccccct
    13141 ttattttcag aattaataaa ttctttttta ttttttgaat atataatcta ttcaagttat
    13201 ttatatttta tatcatcatg tggatttatg caatattcat tacaatattt tttgttattt
    13261 ttaattatag tgattaaaaa agtattttta ttgaaatatt gttgcaatat ttattatttt
    13321 attctataat ctagttaaaa taacacataa tattttaata tttacacata atcagtcatt
    13381 caaaaattta aatttaactt tttaaggagg ctttacaatg aaaaaaacat atctattcag
    13441 tcctggacca gtaatggtta cagataaagt cagaaattca cttcttcact atgatatatg
    13501 ccatagagga aatgagttta tggatttatt caaagataca caacaaaaaa tcaataaact
    13561 atataatgca acttctaatt actactcatt aattgtatct ggctctggaa catctgtaaa
    13621 tgaatgtgta ctatcttcta tctttgacaa agaagatgca gttctgctag ttagtaatgg
    13681 tgtttttgga gaaaggctag aagaaatatt atctacttat aatgtaaaaa catataaacc
    13741 taattttgaa tgggcagaat acccggacct agacatttta gaaaaataca taattgaaaa
    13801 ttctgatata aaagtaattg ctatggtgtt ccatgaaact agttcaggaa tgataaatcc
    13861 cgtccctgaa ctaggaaaac ttgctaagaa gtataacaaa atattttttg tagatgcaat
    13921 aagtgcctca gcaggtgaat atattgatgt ggaagagttt aatatagata taataacagg
    13981 tgttggtggc aaagctcttg gagcatttcc aggttctgca tacctttgtg ctaaagagag
    14041 tattttacaa aatattaaag aaaatcaatg taagaatgtg tatcttaatc tttataaaca
    14101 ctataaagtt gctaaatcat cttctcaaac accaaataca cctaatgtta ctttaatatt
    14161 tgcccttaat gaagctttaa ctgagttttt ggaagatgac agtaaaatag aaagatacaa
    14221 agaatgttct gctattttaa ggaatggtat ggaagaattg ggtctaacat ttttacttcc
    14281 agataagtat atgtctaata cagtaacatc tgtatttcta cctaaagaaa tcaatgtaaa
    14341 tgagtttata ttagggctag aaagggaaag tggctatgtt gtctatccag gaaaaggtaa
    14401 attcctagat gctaatatat tccaagtagc aaatatggga gaaatatatc cagaagattg
    14461 ttataaattc ttagatatct taaaatcaaa attggagata aaaaaagtta tataacagtt
    14521 tgtttgtaaa atcctacaat atatcagtat aaattaaata gactgatttt tttaaatttt
    14581 cctataataa attatatttt atatttttgt ataagggtga actataatat gtaaagttca
    14641 cccctatttc aagtcttttt ccttcaatat aatctttttc ttttaattta ttttatattt
    14701 ttgacgcaat cgttttttat tcaatcattt tatattcttg atacaatcat tcttacactt
    14761 aattattttt tatttttgac acaatcgttt tttgttgaat cattttatat tcttgataca
    14821 atcattctta cacttaatta ttttttgttt ttgacacaat cgttttttat tcagtcattt
    14881 aatattttca acacaatcat tcttacattc tattattgtt tgtttttgat acaactgttt
    14941 ttattcagtc atttaatatt cttgacacaa ttattcttac attctattat tttttatttt
    15001 tgacgcaatc gttttttatt cagtcatttt atattttcga cacaatcatt cttacattct
    15061 attattgttt gttttgatac aactgttttt attcagtcat tttatatttt cgacacaatt
    15121 attcttacat tctattattt tttatttttg acgcaatcgt tttttattta gtcattttat
    15181 attttcgaca caatcattct tacattctat tattgtttgt ttttgataca actgttttta
    15241 ttcagtcatt taatattctc aacacaatta ccttttcatt caatcaaagt aatctttaag
    15301 cattattttt caagctcatc aaagtaatta tatacgataa cctcacctgt ctctttatct
    15361 gtagtcttta taatgccctt agctccattt acctcttctt ttgtaaattc atcatctaac
    15421 acaactttga atttatcttc ctttccataa ccttgttgaa attcatctct caaaattgca
    15481 tttttttctg cttttggctt caactcaaat tcaattccag atttcatttc atcaaaataa
    15541 tttaatacta caacctcatt attcatttta ttagttgtct ttataatccc tttagctcca
    15601 tttacttctt cttttgtaaa ttcatcatcc aatgttatat taaatttgtc ttcttttcca
    15661 tattcctctt gaaaatccct cttcatttca ttgtatttag tttcaaactc tttactcttg
    15721 tttaattcca tagctgatac atttgtcaat gatgctccaa ttgccattac tgttaatgcc
    15781 attactatac cacttttaaa tttttttact ttcatatctt acctcctaaa ttaaatgttc
    15841 tcaatgaaat tatataaaga aaatcctaag ttttacagtt tcattcttgt aactaaaatt
    15901 actattatgt aaccaaactt tttgcttcaa ctattcctta aaagttgctt ttttgaggaa
    15961 tagttgaaat taaataatta gattttatct atatttatag atttaaaatc aaatttatcc
    16021 atataaataa atacttattt ttgcaaaata aaaattttta attactccaa tccttcttat
    16081 ttacttaaaa tcctatgata agaaatttaa aagtctttac attctttaac aataatttat
    16141 ttact
//
LOCUS       DACXMZ010000075        15749 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.075, whole genome shotgun sequence.
ACCESSION   DACXMZ010000075 DACXMZ010000000
VERSION     DACXMZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.075"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            131..1330
                     /locus_tag="JD016_09435"
     CDS             131..1330
                     /locus_tag="JD016_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAU4999855.1"
                     /translation="MLHMISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIG
                     NPSVEPPETIKAVINDILNEESPNLVHGYMNNSGYENVRDAIAEHINKKDGLNLTREN
                     LIMTCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEP
                     DLDALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDE
                     PYREIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSL
                     NVANRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTF
                     YLFPKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKL
                     AKLYNLK"
     gene            1500..2462
                     /locus_tag="JD016_09440"
     CDS             1500..2462
                     /locus_tag="JD016_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAU4999856.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(2584..3246)
                     /locus_tag="JD016_09445"
     CDS             complement(2584..3246)
                     /locus_tag="JD016_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999857.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAYADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDSINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(3346..3431)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3617..6535)
                     /locus_tag="JD016_09450"
     CDS             complement(3617..6535)
                     /locus_tag="JD016_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HAU4999858.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSVLEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDANFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSVKNPGTYYLVEIMAPRGY
                     EKLKDKVKVEIGNEDIGKIVDIGNIENLKQENPPVNPPIPPDTDEPIVNPPVPPSTDK
                     PRKPSSSSDTENTIVINPPVPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(6653..6738)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6865..8043)
                     /locus_tag="JD016_09455"
     CDS             complement(6865..8043)
                     /locus_tag="JD016_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAU4999859.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            8418..8603
                     /locus_tag="JD016_09460"
     CDS             8418..8603
                     /locus_tag="JD016_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAU4999860.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(8668..11442)
                     /locus_tag="JD016_09465"
     CDS             complement(8668..11442)
                     /locus_tag="JD016_09465"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HAU4999861.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(11614..12870)
                     /locus_tag="JD016_09470"
     CDS             complement(11614..12870)
                     /locus_tag="JD016_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAU4999862.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(12942..14444)
                     /locus_tag="JD016_09475"
     CDS             complement(12942..14444)
                     /locus_tag="JD016_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HAU4999863.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(14535..15725)
                     /locus_tag="JD016_09480"
     CDS             complement(14535..15725)
                     /locus_tag="JD016_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAU4999864.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
BASE COUNT         4947 a         2736 c         1921 g         6145 t
ORIGIN      
        1 aatatgcact gtacataaat atattttata taaaagttta tattttcaaa caagtaattt
       61 ttcgaaaatt gtctaaattc agtaaaaata tggtaatata tacataagca atataaatct
      121 ataaaaagga gtgttacata tgatatctaa taaaatgcaa actttagttg caaatagttc
      181 tgttataaga gctatgtttg aagaaggaaa gaaactttct gatatttatg gtgaggaaaa
      241 tgtttttgat tttagtatag gaaatccaag tgttgaacct ccagagacta ttaaagcagt
      301 tataaatgat attttaaatg aagaatctcc aaatttagtt catggatata tgaacaactc
      361 aggatatgag aatgtaagag atgctattgc tgaacacata aataaaaaag atggattaaa
      421 tcttacaaga gaaaatttaa ttatgacttg tggtgctgct ggtggtctaa atattatttt
      481 aaaaactctt ttaaatcctg gtgatgaagt tattgctttt gcaccgtatt ttggtgagta
      541 taaaaattat acagaaaact atgatggaaa attaatagaa gttcctacaa atatagagac
      601 ttttgagcca gatttagatg ctctaaaaaa tgctattact ccaaaaacaa gagctttaat
      661 aataaacact ccaaataatc ctactggtgt catatattct gaagaattat taaaaaatct
      721 tggagaatta ttggattcaa aacaaaaaga atttaataca agcatatacc taatttctga
      781 tgagccatat agagaaataa tttatgatgg tgccaaagtt ccttgtgtac taaaatacta
      841 tagaaattca tttataggat actcttatag taaatcatta tctctacctg gtgaacgtat
      901 aggatatatt gttgctaatg ggcaaatgga tgattttgat gatgttatgt cttcattaaa
      961 tgttgcaaat agaatacttg gatttgtaaa tgctccttct ctttttcaaa gggttattgc
     1021 aagaagttta gatgcagaag ttgatgtaaa tatttataaa aagaattttg accttctata
     1081 taatagttta atcgatatgg gatattcttg tgtcaaacct aatggtacat tctatttgtt
     1141 cccaaaagct ccaattgaag atgataaaaa attctgcaat gatgcaaaac aatttaattt
     1201 attattagta ccaggttctt cttttggttg tccaggtcat tttagagtat catattgtgt
     1261 atcttatgat aaagttaaaa gttctttacc agcatttgaa aaattagcca aattatataa
     1321 tttaaaataa ctaaatcaaa gtaattcaaa taattaacta ccttagtata gaatacttac
     1381 attctatatt aaggtagttt ataattttat gtactatatt ttatacatac catattattc
     1441 caatatgctt gaatattctt atctatagta ctaatataaa ttacaaggag gtgtttgact
     1501 tgaaaaaagg ttatgtttat ataatactga ctaccttatt ttttagttca atggaaatta
     1561 gtctaaaaac tgtcactaat gactttaatc ccttacaaat aactttatca agattttttg
     1621 ttggcggttt agtcttacta ccttttgctg taaaaagatt aagagctctt agtctttcta
     1681 ttacaaaatc agacttaaaa tactttgcat ttttaggttt tatgtgtgta gtagtaagta
     1741 tgtcattgta tcaacttgct gttctaaaca caaaggcatc tgtagttgct gttttgttca
     1801 gttgtaatcc agtctttgta atgcttttag cttacttaat tttgaaggaa aaaatatata
     1861 ggcacaatgt aatttcactt atattagaag ttcttggaat tatagttata ataaatccat
     1921 tgcatacaaa actaactctt agtggaatat tcttaacatt atcttctgct attatttttt
     1981 caatgtatac tgtatttggt aaaagaaaaa ccttaaaatt tggtggaata gttgtaacct
     2041 gctttagctt tatatttggt agtttggaaa tgttgatatt ggttttactc actaaaatta
     2101 attttatcgc agatatatta aataaaaata agcttcatgt atttgcaaat atccctattt
     2161 ttagtggata taccttacac aatatgccca ttatgattta tgtattcgta tttgttacag
     2221 gtgttggtta tgcactttac tttatggcaa tggaagctac atctactagt cttacatctt
     2281 tagtattctt ctttaaacca gtaatatctc caatactagc attattaata cttaaagaaa
     2341 ttatccctat aaacatggtt ataggaattt tattgatagt aattggttca ataatttcta
     2401 ttatacctac gataattact caaatgcata ataaacacgt tgaagccttg tataaggagt
     2461 agtaattgat aatctatcat agttatacat aagacaaaat ttattagatt taaacgcaat
     2521 ataaaagaga tgctattata atagcatctc tttatttata tttattttaa atttattaaa
     2581 actctattta gctaaatttt gcaaaaagct ataagtttgt ggacaagctg attttaactt
     2641 actatttgta tcttgattta aatagtaata tgcaaatgat tctgcaaaaa attcttctgg
     2701 atatacacct aagtagttta cattacctaa agaacgacct tctttagcaa atatttgttt
     2761 gaattgtgct gatttagata tatcatttaa tactatatgg tctattgcat gtgctgtttc
     2821 atgtaattct aaatttattg aatcatgccc tttacctttg ttactaaatc ctattctaag
     2881 agctactgta cttccaccta aacctggaac atcatcccaa gtttttccag taccttccca
     2941 tcccttagga actacacctt ttaaataagc atactctttt tcatctgtta actttccact
     3001 taataatttt atttttaagt tattttgttt taatgcattt aaatactttc catctatatt
     3061 agctaatcta tttaccatag cattagcttc gtttttatcg taatttccag ttggaaaaac
     3121 tactatttga ctaagtgtgt ctttattttg ttgtatagta gtactatctg catatgcact
     3181 tactggaact gaacttatta agaatattga tattatagct attaataatt ttttacttgg
     3241 tctcatatta ttccctcctt tgagttaggc cgggagtaat aaatttcaaa ataaaaaaga
     3301 tacccaagag tatctttgta tatacaaaaa gaaattttta ctcttttcgg caactggctg
     3361 gcatggttta ctaaacttta gcccctatag ctttgcgtga ctaggtttcc ctagttttgc
     3421 ctcatttaat tttatattta tattatagct tatatctttt taattgtcta tatccctttg
     3481 gaactttttt ctaaaaagtt attatttttt tacataaatc ttcaaaaaaa aatatcttct
     3541 aacaatattt tcattattaa atattgtttt agaagatatt ttttaatata acttatattt
     3601 tttataatgt tagggtctaa tttgtatttt tatttcttct taatacgata agtcctacaa
     3661 tagctcctat aaccaaaagt atttctccaa taattgtagt actatcccct gtttttggtg
     3721 gtactggtgg atttaccaaa gtatcatctt taactttatt atttttttct tctgacactg
     3781 atggctttac tacttctggt attggaataa tctgtttaac aggagtttct atcatctctt
     3841 ctgatggtgg tactggcgga ttcaatacct ctggtattgg cggatttatt atatcttctg
     3901 atggtggtac tggtggattt ataactatag tattctctgt atcagatgaa cttgatggtt
     3961 tccttggttt atctgtgctt ggtggtactg gtggatttac tattggttca tctgtatctg
     4021 gaggtattgg tggatttact ggtggatttt cttgttttag attttctata ttcccaatat
     4081 ctactatctt accaatatct tcattaccaa tttcaacctt aactttatcc ttcagttttt
     4141 catatccacg tggagccatt atctccacta aataatatgt tcctgggttt tttacactag
     4201 attgaattat accatcttca tcactaacta aatccttaac tactatattt ccaaatgcat
     4261 cttctaaatt gaacttagca cctgaaagta catttttatc taaagcatcc acttttttag
     4321 cttcaaaagc ataaccttgc ttagtagtct ctactcctgg aggacttgaa ccattaatct
     4381 gagccacatt tcgtatagtc tccccaactt ttacatttgt gaagtttgca tcaaatgtaa
     4441 cccaactttg cacaggttcc ccttctttta tctgtttaaa ttcgtattta tttactaatc
     4501 ttattgtatt ttttccatct ccagcttctt ttttaagttc aagattatca gaatatttta
     4561 tattagatat ttctagaact gagtttactt catctaaaag atttacttgt cctgcatcat
     4621 aaactccata agttgacata ttgattttgt atttaacaat ctggttatca ctactgttta
     4681 aaactttttt atcaacttct ttttttgcgc ttattaatcc agaatttttc ttaaagttta
     4741 tctcatcatt tttatcaatt tcactattat ttgcatcaag aacagctcta tttatatagc
     4801 tatcttcaat actaattacc tttaatgtat aagctataaa aaactttgat tccacttctt
     4861 ctttactgag attaattgta aatccagtta taactttatc tacttgccca ctttcatttc
     4921 ttttttcttc tactttatat gaaatattat tggcaatttt ttctttactc caaggagtcc
     4981 agctcccatt cattggaatt tctacaactc taaaatcatg cccagcttct cctgaagtag
     5041 ttaatctcat gccttctggt atattatctg ttataacaac attacttatt ggtgatttat
     5101 catcatttat tgaaatctga tagtttatta cttgaccaac ttgagtaata tttgttgctc
     5161 ctgaatgtcc tttagtaaga agataatcat ctcctctatc aaatatactt acagtagcat
     5221 catcttcttg tatcttctta ccatcagatt caatcctagc tacattctta tatttattac
     5281 catgttttgc atttttgtca ctgattctag ccttatactt aacagtatac gatttatcta
     5341 tatttccaaa attaactgaa atagaatttt tactctgttc aatattaaaa tcactaactt
     5401 cttttccatc acttgttact acacttacag aaccatttat caatgtcaat ccttcaggaa
     5461 gctcatcata caaaataata tcttttaact cttcctgctt ttcatttact tgtatactgt
     5521 aatttatatc tttattttta actggcataa atacagtagt tgattttttt ccttcaacaa
     5581 gctttttaag tgttccttta gcatctggaa catccgtttt atctccaggt tttatttcta
     5641 catctatatc tccacttcca ctttctattt ttccagtagt ttcaatatgt atttttgcat
     5701 tagtatcttc atcaaccttt ttaacatatg cttccaaacc tattttacct tgcattacac
     5761 tttggccatt aggtaattct ctaaatgtaa gtgtatactc tccattactt tctgttttat
     5821 caaagcattc acttgtataa tctaaattaa ctttattgaa tacatctggc accttaaaag
     5881 ttattgaatc tcctgcttgt attccttttg atgttggagt ataagttaaa ttaaatgata
     5941 tttttttcat tgattccgtt tctattgtat tgttattaat ttttacaaca aatccatagt
     6001 cttcattttc ttcattaagt tcttcttcta aaactttcaa taaattttca tcttctaata
     6061 gattatcttc ctctggagtt ttttctgtat ttttattgtc tagtggtttt tcatcttcta
     6121 aagcttgttc tgacttttta ttatctgttg acttatcttc ttctggtatc tgctctggct
     6181 ttttattatc tattggcttt tctccttctg gagtttgctc tggcttttta ttatctgttg
     6241 acttatcttc ttctggggtc tgttctggtt ttttattatc tgttggtttt tcttcctctg
     6301 gggtctgttc tggtttttta ttatctgttg ccttatcttt ctctggagtc tgttctggct
     6361 ttttattgtc gctttccttc tcctctccag ttgatgaatt agttgaacca ctttgaatct
     6421 gactattctc tcctaattct gaagcataag atacagttgg atatattgta gacaccacca
     6481 tacttaaaat cataattaaa ctaattaata atgcctttct atttcctttc ttcatgtaaa
     6541 tctcacccct ttaggttacc tataatttat aatctaatct tccattaata tcgtatataa
     6601 agtaattttt actaaaaaat tatacaaaaa aagctatgaa aataaataaa tttatattca
     6661 tagccggttg caccactgac tcagtatatc cataaggtgc ccaataatag aatatacttc
     6721 aacatctcta taatatttca taaaaacaat taaaattgct ttatttatat taaaaataca
     6781 attatatact tctattatat tatgcctcta tactattgtc tatccttttt acatttttta
     6841 ccttatgatg agattaatta ataattaaat aaaatcctta tctacaacaa ttacacaatt
     6901 atattcagga tttatctcct taactaattt tgttatatct tcttttaagt catcttctga
     6961 catgtttttg gaaaattcgg aaggattaac tactatatca aatattaaat tcttcttgct
     7021 tccttttcca actattctaa aatcatgcat agattttaca agactatttt tcttaagtat
     7081 ttcttcaagt tcttttttgg ctgacataac ttctttatct tcaagacata ttgggtccat
     7141 gtgaattact agatatatat ctaatttttc agatatttct ctctctgcag tatctataat
     7201 ttcatgtatt tccattatat ctatgttgga tggaatttct gcatgtatag atgcaataca
     7261 tctaccaact ccatagttat gaataattaa atcatgtgta cctattatat atttataaga
     7321 taacaatagc tctataatag aatttaccat ttcttcatcc ggtgcttctc ctaaaagagg
     7381 atttatagta tcttttacaa gagagaaacc agcataaagt atcactaatg atactacgat
     7441 accaacatat ccatctatgg gaaaattagt aaatctagct acaataaatg atataactac
     7501 acaacttgaa gtaaatacat ctcctaaagc atcaagtgaa actgctttta aggctgaaga
     7561 atcaatttta tttcccataa atttattaaa acgactcaac cagattttaa tcattataga
     7621 tgcaattaaa agtataagtg gtattacttc aaattttaca ggaattggat ttactatcct
     7681 ttctatagat gactttacaa attgaagtcc aaccaacatc accataaatg ctactattaa
     7741 tgctgataaa tactctattc ttccatgacc aaaagggtgt tctttatcag ctggcttact
     7801 tgctaattta aaacctatca tagttattgc tgaagatgcc atatcagaaa gattattaaa
     7861 tgcatcagcc attatagcta tactagatgt gagcattcct atgaatacct ttatcacaaa
     7921 taaaagtaaa ttggataata tacctactac accagctaca taaccatact tatttctaac
     7981 atctgtattt tgaacgtttt cgctatctct gataaatgtt ttgactagga ttttggaaaa
     8041 catgaaactt acctccctgc cctttatttt attaacaaat tttataatct atatctttat
     8101 ttatgataac ataatttata tataaaattc ttaaaaatca ctctacttca gtaaaatatt
     8161 attctatata tctatttaga tacgaatttt atatttagta aagtttttat ggcttagtgt
     8221 ctgtttaata gattctatat aagtttttac aaagattatt tattatgata atatcatttt
     8281 tcccaagtat tacttagaat atgatttttt tgttttgtct gtacatattt taaatttata
     8341 ctatatttat ctatatttta taaccttatg atacactata actataagat tttaaatgga
     8401 aaggaatgaa ataaatcatg ttttttatca gtgatattta cacaaaatca cctattaaat
     8461 ttgatactcc tttgcaaaaa gaggcataca aaatattaca aaaattagat attgactttg
     8521 aatgtgttga cacagatgaa gcaattacaa tggaagattg tgttcagatt aataaaaaat
     8581 taaatatgaa aatggtaata taaaacaaca ttaaacaaac aaaaaccgca aagttacaat
     8641 atatagtact ttgcagtttc ataagaatta atttttacta ttaaagttca tagttctact
     8701 atttttcatt gttctaagaa ttagctttac aaattcattt gacaatagga tagtggatgc
     8761 aaatagtatt atctttatcc acatatcaaa gtccaaagaa acagaattaa agaaactctg
     8821 gaacacttgt gtaaatatta tttgaattat ccctgtaact actattattt gcaatgccag
     8881 tttattttta aagaaatttg gtattgtact atttaaacca aactctctac aatttaatgc
     8941 attaaaaagg gcactgaatg caaataaaga aaatataaca gtaccttgtt cttctgatgt
     9001 agctcccaat atattaaaag ctgattgagt aaatacaatt gtaattataa gaattgcatt
     9061 tataataata tttacaaaca tagacctagc tattattcca gaatgtctat ttattggttt
     9121 tcttttcaat acataatctc taacaggttc taatcctaat gctagggctg gaggaccatc
     9181 cattattata tttacccata ataattgtat tgtagtaaat ggcatatcct taccagtaag
     9241 ttgagatatt atagcaatta taaatgctac tatatttaca gtaagctgaa actgtataaa
     9301 tctttgaaag ttctcatata tacctcttcc ccattttatt ccctctacta tagtactaaa
     9361 gctatcatca gtcaatatta tatctgctgc atttttagat acttctgttc ctgaaatacc
     9421 cattgcaatt ccaacatctg cttgactaag tgctggggca tcatttatac catcacctgt
     9481 tacagctaca acttcgccac ttttttgaag tgctgaaact attctcattt ttgtatctgg
     9541 tttacttctt gcaacaatag atattccttt gatttcttct ctcaattctt tatctgtcaa
     9601 tacatctata tatgttgctt caactgcctt tttcccatca tttaataatc ctaattcatt
     9661 tccaatagct gttgctgtat ttatattatc tcctgtaagc atttttacat ctactcctgc
     9721 attaaatgct ttatcaatgg aatctttgac cccttctctc aatgggtcca ctattcctac
     9781 aaatccacta aaaactaaat tatcttcctt catataactt cttgaatttc ctattaaatt
     9841 caattcacct tctgaagtca ttgcaacttc tgttttagaa ttactcattt ttttataagc
     9901 aaatcctaat gttctcatag atttaatttg taacttctta atctcatcta aaatactttt
     9961 ttctactttt ggagttattg gaacaatatt ttttccttgt tgtacataag aacatttttt
    10021 taataatact tctggagcac cttttgaaag taatatatct gaatcctctt gacgaattaa
    10081 tgttgacatc ttcttttctt ctgaactaaa tggtatttgt gatatcaaat acgtttgttt
    10141 tctcatttca ttatagttct tatcattatg atataaaagt aatgcacact ctgttgcgct
    10201 tcctatatat ttaaatgaat tatcttcttt ttctatatct gcagttgaat ttactataca
    10261 gttttgttca aaatagctat tactttgata ctctcctcca cttatatatt taccatcaac
    10321 atatgctacc tctaccatca ttttgttttg tgttaaagtc cctgttttgt cggaacatat
    10381 tacagaaaca gaacctatag tttcacaggc ttctttcttt gttactaatg catttatctt
    10441 tgccatcttt tgcattgtta tagctaaggt aatatttatc atagttggca aaccttctgg
    10501 aacagttgca actattaatg caatacaaac catatatgca tttttagcag gttctaatga
    10561 ttgtaagaaa gataatacac tgcttgtatc taaaattaac ataccttttc tctgcatctg
    10621 tataatcata aacatacata ataaagtagc tattacgcct gatattcctg ctattttagc
    10681 acccaacttc cctaatttaa tttgaagtgg agtttgctgg tcttcatttg ataaattctg
    10741 tgctatctta cccatctctg tcttatcacc agtatgagtg acaaccatta tgcccctacc
    10801 atatgctaca agagtccctc caaataccat attgacttgc tttgcaggta ttgcatcctg
    10861 ttcgattatt tctgttttag aataaactac ttccatatct aatacagcat cagcattctt
    10921 agctacatca tcagattctc ctgttagcat atcttctcta agtttcaaat ttatagactg
    10981 tataagtctt ccatctgctg gtatcatatc cccagtttct atatatacaa tatctcctgg
    11041 tactaaatca ttttttgata ttttaattat cttcccattt ctaagcacct ttacttctat
    11101 attttctgtt aattttgaca gggcatgagc tgctttttta gattttcctt cagttattat
    11161 gcctatagaa attccaataa aaatagcccc taaaatacct aatgcgtcat gtaattctcc
    11221 cacaaaagaa cttattactg ctgctcctat gagaattaat atcataggtt ctgtcaaact
    11281 ttctcccaac tcgtcccaaa atgttctacc ttcttttata gtaaattcat ttaatccata
    11341 tctaagcttc ctttcttcta cctcattatc atcaagtcca atttctggat ttgtttttaa
    11401 gtattttaat acttctttgg taggtttatt ataatacctc ataactttat cccccttgtt
    11461 aattatctat aaggaattat atgcttatga tagcttgtat attacttttc aaaaaaaata
    11521 aggtgttgat taatcaacac cttatagtgg ttatatatta ttaaatttta tgttacaggc
    11581 tttataaatt gtgagctata caaatcaaaa tatttaatct tcaaaatacc cttctggata
    11641 taatggaaac ttagatataa gttcttgtgc ctctgcttta cattcatcta atacttcttt
    11701 attatcaata ttttctgcta ccttattcat tatccctgct atctgtataa cttctttttc
    11761 tttcagacct ctttgagata ttgatgtaag acctatacga acaccactag ttacaaatgg
    11821 tgattgtggg tcgtcaggta taacattttt attaactgtt attcctacat attcaagagc
    11881 tttttcaaat tgttttcctg taagattctt acttcttaag tcaaccatta ctatatgatt
    11941 atcagtacct ccacttacaa tactaaaacc atgtgaaatc agttcagaag ctagagtctg
    12001 agcatttact acaacttgct ccattatctt tttaaattta tctgtactta aatataatag
    12061 tgaaaaggct tttgctgcaa ctgtattaag atgtatagac cctaatgttc ctggaaaaac
    12121 acctttatcg agtttctttg catgttctgc tttacaaagt acaataccac ttcttggtcc
    12181 acagaaagtc tttgtcgtag atgaagaaac aaaatctgca taaggtacag gactaggtat
    12241 tactttcgct gcgactaatc ctgcaacatg tgccatatct accataaagt aagctcctac
    12301 ttcttttgca acctttgata ttctttcata atctattaat cttggatatg aacttgcccc
    12361 tgcaattata agttttggtc tacattcttt tgcctttgct tcaagagcat cataatctat
    12421 acgtccagta tttgggtcta ccccataaaa ttcatattta taaatgtcat gtaaaaaatt
    12481 aactgcgcta ccatgtgtca agtgtccacc ttggtcaaga cgcatactta aaacagtgtc
    12541 atttggtttt aatattgatg aatatacaca atagttagct gtagaacctg aatacggttg
    12601 tacgtttaca tgttctgctc caaatacttc ttttgcacgt tttattgcta gggtttcaag
    12661 tttatctgct tcttcagaac ctgcttgaaa acgagcccct ggatagcctt cttctgtttt
    12721 atttgtaaat acacatccac ttaattcaag tacttctgtt ggtgctgtac tttcagaagc
    12781 tatcatctca atattgtgtt cttgtctaac caactcatca gctacaatct tgtataactc
    12841 tgggtctgaa atttttgttg tttttaacat aataatcctc ctcttaatta aagaatatat
    12901 tttattagtt taatcttcat tgataaattt attataacgg tctataaaac gtaagatatt
    12961 cctccaccag gtcctaatgg tatgccaata cccatgaata gtaacaatat tattatttct
    13021 attaatgtaa ataccactga gtatggaatc atattcgata tgacagtacc tatacctata
    13081 tctttatcat atcttctggc aaaggccaat ataactggaa aatacggaaa aagtggactt
    13141 attggatttg ttgaagcatc tccaatacga taagctattt gagttaatgc tggattaaag
    13201 tctagtagca taaacatggg aacaaatata ggtgctaaaa tcgcccattt tgcagaagca
    13261 cttcctacaa ataaattgat aaatccagat agcaatataa atcctattat caatccatag
    13321 ctaggtattc ccgactctgc tagtaatttt gctcctgtta ttgataaaat tgttcctaag
    13381 ttactaagat taaataaatt aataaactgg gatgctataa atgctaggac tatatatcca
    13441 cccatatcac tcatactact acccatcata gatactaaat ctttgtcact ttttatcttc
    13501 ttacttactt ttccatacac taatcctgga gttaaaaata taatagtgat tataggaact
    13561 atacctttca ttaaaggtgc attactagat agtatagaac cagtctctgg atctttcata
    13621 aatgcattgt cacctataca taaggcaact atcactacta ctacaactaa caatgataat
    13681 aaagcgtatt ttataccctt attttcaagt tcactcaatt cctgtatatc cttatcattt
    13741 gttgaatcat tatattttgt aaaacgagga gctataaatt tttcagttac aaatgctcct
    13801 gcaaaagtta aaactatcgc tgatataaaa gcaaaataaa gattcattgc tggattgcct
    13861 tggtaagatg ggtccatcat ttgagctgct ggtattgtaa atccagctaa caatacatct
    13921 gtagtactaa gcataacatt tgctgcaaat ccccctgcaa cacctgcaaa agctgcaaac
    13981 atacctacaa gaggatgtct tccaattcct aaaaacacaa gtgctgcaag tggtggtaat
    14041 attataaatc ctacatctgc tgctgtatgt gacaacatac ctgcaaatac gattgctaca
    14101 gttacaagtt tttgtggaac tttacttata ccctttttca ttaacacttc cataaatcca
    14161 gatttctcag caacacctgc tccaagcatt gtaaccagta ctaatcctaa aggagcaaat
    14221 gactgaaagt ttgtaactat gctacctaaa tatgattgaa tctgctcttt ctctagtaga
    14281 tttactacct ttacaacttc ttttgtagaa ggatgtattt cttctactcc taaattagat
    14341 ataactgctg acaatattac aacaattata catagtaata aaaatatggt tactgggtct
    14401 ggaagtttat ttcctgctct ctctacaaca tttagtgccc tcatcaataa tccactatct
    14461 ttgttttttt cctgtgaatt aattttcctc aaactaatac cccctcataa actttatctc
    14521 cagaatgctc tatcttaaat tttatttttt aatttatcca tataatttat aacagactga
    14581 catagtacat ttgtacctac caatatagaa tttttatcag ctttaaattt aggattatgc
    14641 cactgatata tacaatcttg ttctttgttg cctaccccta accagtaaaa aaatcctggg
    14701 gctttttcca tgaatataga aaaatcttcc cctcctgtag aaggtattgg atcaactata
    14761 gcatcttctg aatacaaatc acacacagac ttacaagcta ttgcgtacat ctctttacta
    14821 tttataacag ctggtaaatc aaataaataa tctagctttg cagttgcttg atatccacga
    14881 gctatctcac aaactatatt ttctaaattt tctgatattt tctttcgtac ttcattacta
    14941 aagcttctac atgtgcctgt catatctacc ttttctgaaa ttacattatt tgcagtccct
    15001 ccatttatag tacctattga tataacagct gattctaatg gacttatgtt tctgcttact
    15061 atggtttgaa tacccattat aattgcactt gatgctacta tagggtctat tgttctgttt
    15121 ggtatacctc cgtgacctcc aaatccattt acttctatct taatagtgtc tactgctgcc
    15181 attaacccac ctaacttaac acctattttt ccacaaggta tatctggatg attatgaagt
    15241 ccaaatatag catcaacttt tggattttcc attactcctc gttccactaa catcttcgca
    15301 cctttattga cttcttcggc tggttgaaat ataaacttta catttccatg aagactatct
    15361 ctaatatgag acaatacata tgcacatcct acaaggcaag acgtgtgtat atcatgccca
    15421 catgcatgca ttataccatc atatctggat ttatacttaa tatcaacctc ttcatatatt
    15481 ggcaacgcat caatatctcc tcttagtgct actgtgggac catcatactt cccttttaat
    15541 aatccaacaa ctcctgtttc cattcctaaa tctaatattt ctatgtctaa acttactaat
    15601 aaatctttta tagcttttgt agtcctaaat tctttaaaac ttaattcggg gtgcatatga
    15661 atatctgtaa aaaaatctaa aatatcttct ttatactttt ctgagattaa aataagttca
    15721 tgcatactaa cacctctcca aaattttat
//
LOCUS       DACXMZ010000076        15691 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.076, whole genome shotgun sequence.
ACCESSION   DACXMZ010000076 DACXMZ010000000
VERSION     DACXMZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.076"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            123..1340
                     /locus_tag="JD016_09485"
     CDS             123..1340
                     /locus_tag="JD016_09485"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HAU4999865.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1435..1815
                     /locus_tag="JD016_09490"
     CDS             1435..1815
                     /locus_tag="JD016_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAU4999866.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2099..2581
                     /locus_tag="JD016_09495"
     CDS             2099..2581
                     /locus_tag="JD016_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAU4999867.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSDVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2602..3417
                     /locus_tag="JD016_09500"
     CDS             2602..3417
                     /locus_tag="JD016_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU4999868.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3467..5017
                     /locus_tag="JD016_09505"
     CDS             3467..5017
                     /locus_tag="JD016_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAU4999869.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLSVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5114..6466
                     /locus_tag="JD016_09510"
     CDS             5114..6466
                     /locus_tag="JD016_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HAU4999870.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6597..7355
                     /locus_tag="JD016_09515"
     CDS             6597..7355
                     /locus_tag="JD016_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999871.1"
                     /translation="MNIINKIFWEEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7402..8121
                     /locus_tag="JD016_09520"
     CDS             7402..8121
                     /locus_tag="JD016_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAU4999872.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHISKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8245..9645)
                     /locus_tag="JD016_09525"
     CDS             complement(8245..9645)
                     /locus_tag="JD016_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAU4999873.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9649..10539)
                     /locus_tag="JD016_09530"
     CDS             complement(9649..10539)
                     /locus_tag="JD016_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAU4999874.1"
                     /translation="MTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKATFYKHF
                     ETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRTTINTYI
                     RENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDALIHQFI
                     YIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYFSLHNKS
                     LFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAYLEKEDT
                     LRPKVLLLNSIKNQLKKETL"
     gene            10818..11465
                     /locus_tag="JD016_09535"
     CDS             10818..11465
                     /locus_tag="JD016_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAU4999875.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11790..14429
                     /locus_tag="JD016_09540"
     CDS             11790..14429
                     /locus_tag="JD016_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAU4999876.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQVILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14487..15590)
                     /locus_tag="JD016_09545"
     CDS             complement(14487..15590)
                     /locus_tag="JD016_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HAU4999877.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
BASE COUNT         5911 a         1894 c         2743 g         5143 t
ORIGIN      
        1 aggttttttg tgattaatta aaaaattata aaaatcgttt acatgttgat ttttaaaaaa
       61 aaaagatata gaattatata gaaagtaata aacaaaaatt ggatttacgg gagtggaatg
      121 aaatgatgaa tgttacctta acagatgtaa aagaggctag agagaccatt aagaacatag
      181 taaaaagaac tgacttactt gaaagtgtta aacttagcga aaaaacagga gcaaatgtgt
      241 tctataaatg tgagaatctt caaaaaacag gttcttttaa attaagagga gcttgtaaca
      301 aaatagcgag tttaacagat gaagaaaagg caaatggagt tatagcttca agtgcaggga
      361 atcatgctca aggagttgca ttgggagcaa aaatgactgg tataaaatct acaatagtaa
      421 tgccagcaac tgcgccttta gcaaaagtta gtgcaactaa aggttatgga gcagaagtag
      481 ttttaaatgg tgcggtttat gatgatgcat atgcaaaagc tgttgaaata caaaaagaaa
      541 caggagcaac tttcttacat ccattcaatg ataagtatgt tatagcagga caaggaacta
      601 tatctcttga aattttcgaa caattagaca ataaagttga tactatacta tgcccagttg
      661 gtggaggagg aatcatctct ggtgttgctg ttgcagcaaa agcattaaat cctaatgtaa
      721 aaatagtcgg tgttcaaact gcaaatatac cttcaatgaa agagtcaata aaaaatggaa
      781 aagtaacaac agcatttaat gatactacaa tagctgatgg tatagctgtt aaaactccag
      841 gagatttaac ttttgaaata ataaatgaat tggttgatga aatagtagtg gttgaagaga
      901 cagaaattgc agaatcaata ttatttatga tggaaagtca aaaaatagtt tcagaaggtg
      961 ctggtgctgt atgtacagct gctatattaa gtggtaagta tgtaccagca aaagatgaaa
     1021 atgtagtttg tataatatct ggtggtaata tagacataaa tacgttatat agaataatag
     1081 gtgttgcttt agctaaagaa ggaagaagat attcatttag tacaattatg gaagataagc
     1141 caggaaactt tgctgagtta actagaataa taagtgagaa tggtggaaac attttaagtg
     1201 ctaatcaagg taaattatca gcaggggaag ctctaggaaa acaaagtgcg gaatttatat
     1261 tagagacaat agattatgac catatagcta gaataaagaa agctatagaa gaaaaaggtt
     1321 ttaaaattat agaattataa taaaaagtgt atataatata caagaaaaat agtataatag
     1381 tgtatgaaga tacattttat gtgtattaga ttaacgaatt aggaggattt taaaatgaaa
     1441 catgaagtaa tacatactaa tgatgcacca gcagctttag gaccatattc tcaagctata
     1501 aaagctggaa acttgttatt tgtatctgga caagttcctc ttgtaccaga gactatggaa
     1561 gtagttgaag gagatgtaca agctcaaact gctcaatctt taaaaaatct taaagctata
     1621 ttagctgaaa gtggagctga tttttcaaat gtagttaaga ctacagtatt tataaaagat
     1681 atgaatgaat ttggggctat aaacgaagtt tatgctgaat attttggaga aaataaacca
     1741 gcaagagctt gtgtagaagt tgctagatta ccaaaagacg ttaaagttga aatagaagta
     1801 atagctgtat tataaaaata caatattttt aatttgaaaa ggctgaaaaa ctatattcta
     1861 gtgattcagc ctttttataa aaaaataaat cacaataaag aacatgataa atattgtcga
     1921 attaaaaaat aaaattaaaa taaatattgc aatatttttg agaaggtttt ataatgagta
     1981 tataatttga aacgtgtaac tattaaatta ggcatgagtt cttggatatt tttatcctga
     2041 ggattcatgc cttttttgta ttgaaattca aaaatctatt taataaggag gaagtactgt
     2101 gttaaatttt ttcaagaaaa ataaaagcta taagttacat gcagtagtga gtggtaacag
     2161 cattaatatt gagaaagtaa acgattctgt gttttcaaaa aagttaatgg gagatggagt
     2221 tgcaattatt cctaatagtg atgttgttgt agccccttgt aatggtaaag taactgtgct
     2281 tacagaatca aagcatgcct ttggtatggt ctcagatgag ggtgttgaaa tattagtaca
     2341 cataggaatt gatacagtta gtttacaggg tgaggggttt aaaaatgaag tgtctcaagg
     2401 agatactgtt aaaaaaggta gtccaataat aagttttgaa agagaaaaaa taaattctca
     2461 gggaattgat tgtacaacaa taataattgt attaaatcat agcgaatttt cagaaataaa
     2521 ttgcatggta gaaaatgaag ttgttgcagg gcaagatact gttattgaaa ttatgaaata
     2581 ataggaaggg attaattcaa tatgagagta gtaaaaatct ttaataataa tgcattatct
     2641 acagtaacta atgataggaa agaagctatt ttactaggtc ttggtatagg ttttaataaa
     2701 cgtccaggag ataaagttaa tgaagataaa atagaaaaga tttattatgt tcaagaccac
     2761 atgcaaacaa aatttttaga attattaaaa aatgttagtc cagaagtgat ggatgcttca
     2821 caacaaatta taagtcttat accaggaaat gagggaaaat ttaataataa agggatatta
     2881 tctttagtgg aacatattag ttttgcaata gatagaatga aaaataatgt ttttttacct
     2941 aatttaatgc ttttggatat aaagatgatg tattcaaaag agtttgaatt aggagtaaaa
     3001 gctttagaaa ttatatatag agtatgtcat atccatttgc ctgaagatga agcaggttac
     3061 atagctttac attttgtcaa tttacaatca aatgataatt tggcatatga tactttaaaa
     3121 tttgtaaaag gtagtattga tttgattaaa gaatgttatg gattagaatt agacgaatcg
     3181 agtttaagta cattacgttt tagaactcat cttaaatttc ttgctcaaag aatttttaag
     3241 aatgaaattt gtcaggatga taaaatgatt gaaatgtatg attatttaat taacaatcat
     3301 cctaaaaata aagaatattt agaaaagtta aatgtatata ttgaaaagga gtttaggtat
     3361 aaattagata aacctgaaaa aatttatcta ctactacacc taacaaaaat cttataagaa
     3421 tgagatgaat tagtaataga aatatataat tttagggggg ataagattga aagaaaaact
     3481 acttggagga ctggaaacat ttgcaaaggc aatggttcag ccattaatgt atttatcgag
     3541 tgcaggaata ttaatggtgg taggtgtact acttacaaat gatatgttat taggagtact
     3601 tccatttttt aaatgggagc caattcaaat atttggaagc ctaatttata actgtataat
     3661 ggctattatt aataatcttg ctgttatatt tgctataggt attcctgctg cactagcaaa
     3721 aaaagagaag cacaaagcag cgttaatagg gtttatgagt tatataatat accttacagc
     3781 ttctaacact atgttaacat cactaggtca actagcaaaa gaaagcaaga tgcttggact
     3841 gatagggact ggacaagcta ctattttagg tattcaagtt gtagatacaa gtgtatttgg
     3901 tggaattatt ttagggtgta tagctggata tgtatttaat cgtacaagca acaaaacatt
     3961 taaaggagct ttacagatat actcgggagc aaattttagt tttatatgca tgttctttgt
     4021 atccatttta attggagttg tgagtaactt tatatggcca ttagctcaac aaggaatagc
     4081 ttctcttgca aatgtcatca aagatacagg tacatttggt ttattcttat atggtttttt
     4141 agagagaata ttagtaccaa ctggacttca tcatttaatc tatacaccat ttcagttttc
     4201 tgatttagga ggagtgctta aagttggtgc agatacagtt tcaggagcct atgctatagt
     4261 tatgacagaa atgaatttat cagttgagcg tttctcagat agtatatact acatggcaac
     4321 tggatttaca aagatgtttg gttatatagg tataggagct gcctttattt atactgcata
     4381 taagcaaaat aagaagaaag ttaaggcaac gattattcca ctaataatta cggcagtatt
     4441 agcctctgta acagaaccat tagactttat gtttgtattc tgtgcaccac ttttattctt
     4501 tgtacatgca gttatagctg gtctttttat agctttatta aaaatatttg atgttactgc
     4561 attatgtgga gggaacttat tagtatcaat tataatgaat actacagtag gcgtagaaaa
     4621 aacacactgg ccagtaatga tgatattagg acttgcacaa attattgtat attttgtagt
     4681 atttagtttt ttaattaaga aatttaatta taaaactcca ggtcgtgaag atgagagtac
     4741 agctacaggc gaagaacaat caaaacaact taatttagat gcaggtatag aaaatataat
     4801 tgatgggtta ggcggaaaag aaaatattaa tacagtagaa aattgcatca ctagattacg
     4861 tgttaatgta aaagatgaaa gtaagattaa tgaagatata attaatttaa cgccaaatag
     4921 cgggattgta aggaaaggaa aagatataca aataattttt ggacttcatg tgcatgaggt
     4981 aagacgtgca gtagaagatt ttttagaagc ttattaaaga tatatattta tatagcaagg
     5041 gtaaataaca gctttaagtg ctgtttacta tttattaaag atacatataa tttatagagg
     5101 aggaataatt attatgatta ttactattgt tggtggagga agcacattta ctccaggtat
     5161 tgttaagtca attgcattaa gagaaaaaga gttagagctt gaggaaattc gcttatttga
     5221 cattgataaa gaacgccaag ataaagtagc tgtagttgtc aactggatac taaaagaaga
     5281 tgttaaaaca gatgttaaac taactgtaac taattgtgaa aaagaagcgt atacagatgc
     5341 tacatttata tttgcacaaa tgcgtgttgg taaatatgaa atgcgtgagc aggatgaaaa
     5401 aattccattg aaatatggtt gtgtaggtca agaaacttgt ggttgtggag ggatggcata
     5461 cggactgcgt acaatacaac caatgataaa ggttattgat gatgtagaga aatatgctaa
     5521 aaaagattat tggatattaa attactctaa tcctgctgca atcgtttctg aagcttgtcg
     5581 taagttaaga ccaaatgcaa ggattataaa tatctgtgat atgccaatag ctattataga
     5641 tatgatagcg gggtcattaa atatcaatga cgttcataat atacgctatg attattttgg
     5701 tctaaatcat tttggctggt ttacttcaat agattataaa catagagact taatggaaga
     5761 gattaaggaa tacataaaag aaaataaaat attattacca aaatcttata tagaaaactt
     5821 aaataaaagt cgtagtacag gaaatcgcca tgctgtaagt agctggatac atgtttggga
     5881 atctgtttat acaatgttag aaagcttccc aaaatatctg ccaaacactt atttaacata
     5941 ctatctttta agtaaagaaa cagtggaaca ttcaaacata aatcgtactc gtgcaaatga
     6001 ggttatggaa agccgtgaaa aagacttatt tgaaggtgtg agacattact tagaaactgg
     6061 agaaatttct gaaaaagcat tttatgcagg ttcacatgga gattggatta gtgatttggc
     6121 agtttcaatt aaaaatgata cacgtagtcg ttttttagtt ataactgaaa atcgtggagc
     6181 tataccaaac atgccatatg atgcaatggt agaaattcca gcatatattg gtaaagatgg
     6241 tccagaagtt attgctcgag gtccaatacc tctattccaa caaggattaa tgatgcaaca
     6301 acttaacagt gaaaaattga tagtagaagc ttatatagaa aatagttatg aaaaagcttt
     6361 acaagcattc acattaaata aaactattcc aagtatgaat gttgctaaag aaattttgga
     6421 tgaaatgata gaagttaata aggaatactg gccagaatta agatagtagg ataaaagaaa
     6481 gggtttttat ttaataatta gatatgtttt cagagtcttt tatcaaaatg tattcacaaa
     6541 catatggcaa tatattaaaa taaagagtga tatttttaga agaatggggt gatgcaatga
     6601 acattataaa taaaattttt tgggaagaag ataggattgg catgattaca aataatttag
     6661 aggcagatac acatagccac tgtatgttac aactgttctt agggatagaa gacagcattg
     6721 aaataacagt caatgaaaaa ttagttaaat gtaattgtat catagtagat aaaaatatct
     6781 ctcattcatt ttcagcaaga aaaaaagttt attactctgc tattattgaa cctacatcta
     6841 tatatgcaga acagcttacc tcaaaaatga atgattttgg ctactggata tgtgacaatg
     6901 atggattaga gaaattacgt caacaaggaa cttttttgat agataactct agtaaagaac
     6961 aatatttaag atttatggaa atgcttaata actatttgaa tatacctata actctcaaac
     7021 attatgatga cagaattact gaattgctca atttattacg tacttgtaat tgtgataatc
     7081 atactatttc aagttttgca gataaagtgt ccctatcagc aagtagatta tcacatctgt
     7141 ttaaagaaca aatagggata cctttaaaaa gctatatctt atttcatcaa atggagtgtg
     7201 cttttagaga attactttct gggaaaaatg ttaccgaagc ttctatgact gctggattcg
     7261 atactccatc acactttgca gggacagtta agcgaatgat gggaatgcct gtttctttat
     7321 ctttgaaaga tagcgaattt ttgaaagtat attaaatatt cttgttatat aatgaagttg
     7381 tactaagaaa ggggctttgt tatgaataaa gaatatttag aggaaacaaa aatgttaaat
     7441 tataatgaac cacaacttaa gcttttggta tcatcaaaga attggttgga attagatgat
     7501 tttcataaaa taaaatctat ttatgagttt gtacagaatg atattttatt tggatataat
     7561 gcttctgata tgttaagcgc aacacaggta ttaaatgatg gtatgggaca atgcaataca
     7621 aaagctactc ttttaatggc attattacgt gcagtgaata tcccttgtag gcttcatgct
     7681 tttgatgtaa caaaagattt tcaacgagga gctacatcca aattgatttc actattagca
     7741 ccaaaatata ttcttcatac ttgggtagag gtattttatc aagataggtg gattgctctg
     7801 gagggtgtca taactgataa aaaatattta gaagcaattc agaagaaatt ttttaatcat
     7861 ggtggaacgt ttaaaaagta tgcaatagct acaaatgatt tgaaaaatac atctattgat
     7921 tgggatggta aagatacttt tatccaaaaa gaagctatcg tgtatgatta tggtattttt
     7981 ccatcaccag acgtattttt ttctactcat tcacaacata tttcaaaatt aaaaaatttc
     8041 atatatgtac atcttatcag aaaaataatg acaaaaaatg tatgtaaggc tagaaacaat
     8101 tatattgata aaaacgaatg agtgtttaag aggtaactat ataggaaata tatagaataa
     8161 ttaaaactgt actggttaaa atgaataaaa aggagttatc acagagcaaa cttaaattgt
     8221 tcataggata actccttttc tgtattaact tctaataatt tgatgtcttt ttataaaaca
     8281 tatagataca attaaaccag taaatgcaag agtagctgct attgaaaatg caacatctat
     8341 tccatgaatg ttagacagca tgtttgaact catatgagct ttcttagtag cacttgtcat
     8401 tatagtaata agtatagcag aacctatagc tccagatatc tgacggagtg tgttattaat
     8461 tgctgtggca tgagatataa gttctctatc taatgtttta attccccatg tagttaatgg
     8521 cattaaaacc attgaaatac caatcatacg aatacaatac atcaatgaca catgaattaa
     8581 agatgtatct ctgcctaaaa aagcgaagga taaagttcct aaaaacaaac atcctgttcc
     8641 taatattgat agagcatgag gtccatattt atctaaatga cgtcctgcaa ttggattgag
     8701 tacaaccatc aatatagcac caggtaacat taatgaacct gaagataatg cagaaaatcc
     8761 tcttacagat tgaacgtaca gaggaatcat taatgtagca gacatcattg aggcatatac
     8821 aatcataatt aatattgtag aaataagaaa tggtttattt ttaaaaaccc tcaattctaa
     8881 aaaaggttct ttagatttta attgacgcaa tgtaaataaa attaatgaca taattcctat
     8941 taatattggt aaatatgtgg caatattagt ccacccataa ttaccttgat tagtaaatcc
     9001 aattaataaa ccaccaaaac ctagagatga tagtactatt gaaggtatat ctaattttag
     9061 tttttgagtt tccccaacat ttcttaatag aataaatgat agtataacat ctaatatagc
     9121 aataggggaa agaaacagaa ataaactatg ccaaccaaat gaatctacta accatcctga
     9181 taaagttgga cctattgctg gagcaaatcc tactgtaata ccaactaaac tcatagctgc
     9241 accacgtttt tcctcagggc ataaatagag tataataact tgtaaaagtg gcattagaga
     9301 accagaaccc attgcctgca acacacgagc aatcaacata gttgaaaaat tattagaaaa
     9361 aagtgcaata atacatccta tagaaaagat agacattgat gtaataaata actttctagt
     9421 actaaatcga tttattaaat aagctgttgt gggtacaata atacctgcaa taagtagata
     9481 aattgttgtt aaccactgac caatatcagc agtaatttta aaatctgaca ttatattagg
     9541 taatgctgtt gacaatagtg tttgactgag agaacttata aaagcaccag tcattacaat
     9601 tgctattatt aaatttcctt ttttattgtt actttgggtt tccataattt ataacgtctc
     9661 cttttttaat tgatttttaa tgctgttcaa taaaagtact tttggtctta aagtgtcttc
     9721 tttttctaaa tatgcaatca ttgcatccat aattaaagaa tcatattgtt gattttttcc
     9781 ctcgacgtat aattttttta ttgcttctag taatttagga ttttcaagga gagaagtatc
     9841 agttttgatt agttcttcta tatcttgtat cgttttaaaa aatagcgatt tattatgaag
     9901 ggaaaagtaa acttcatctt cttgtttatg taaaaacatt gatttttcaa ttaaaggatt
     9961 tagattatcc atattttcta caactaaatc tagaatttgt atagtaaatt tggcaataaa
    10021 gtatacattt atttctcttt tttgtacatg aataatgtat ataaactgat gaattagagc
    10081 atccaataag aaaactaaat caaatataat tttttctatt ttatcaccat agttatcaaa
    10141 taagcttagt ttgtattcat tcaataagtt agctcgtgat tttgtttgca atttacttgt
    10201 agccttacgt tgctcttcag aaaaattttc tcttatatag gtatttatgg tagttctaga
    10261 aaaaatattt ttccatacag caattatctt tctgtttaac ttttctgatg atggaatgtc
    10321 attattttcg tttatggaat gtataatttc taaaaacttt tcatcataat aattgattat
    10381 ttcgcagata aaactttcct tagtttcaaa atgcttgtaa aaagtagctt tagacatttt
    10441 acactctttt acaacatccg atattgaagt attttgaaaa cccttttggt caaaaatttt
    10501 ttgtgcaata gttataatga gcctttgttt ttctgtcact ttattatctc ctttcacgca
    10561 aaaacgttta gtactatata gtacctacaa gttttaataa atatattata catcatttgg
    10621 aaaaaaaatc aatatataga ctgtaatatg aaatttttga aaaatttgtt gttattttta
    10681 gtcgtataaa aaagaaactt aagagtttct taagaattgt ttaaagaagt tgcaataaat
    10741 tagtggtatg attatagtat gaaagagaat gtcttgaaat ttttctgtat caaattccaa
    10801 ttatggaggt gtagtatatg aaaaagaaaa tttgtgtagc aataattttt gctctattta
    10861 tagtttttgt agaaatgttt attgatagct ctagcgaagg aagtaaaatt atgacagctg
    10921 aattaagtaa atacaggaaa ggcgtaaatg tattatcaat aaacaatatt atagtagcga
    10981 ataaaaaata tagtttgccc aaagattatt ctcctcagga aagcagtgaa gcgagagatg
    11041 ctttttataa aatgaataaa gatgctcaaa agtcaggatt aaatttaaaa gcttttagta
    11101 catatagaag ttatgaatat caagatagat tatttaagtc atatgtaaaa gaacatggag
    11161 aaaaagaagc aaatagattt tcagctaaac ctggagagag tgagcatcaa actggacttg
    11221 cttttgatat tggaggagat gaccagtctt gttgggcaaa taaaaagttc aacaatacaa
    11281 aagaagctaa gtggttgtat gaaaatgcat ataaatatgg ttttatactt agatatccag
    11341 aaggtaaaga gaatataaca ggatatatgt atgaatcttg gcactataga tatgttggaa
    11401 ctgaacatag caaaaacttt gcaatgaata atttgacttt agaagaatat ttacatataa
    11461 attaagagat ggtttgttaa aattaggtta aaataaaaaa taacctagac tattagctgt
    11521 gtaaagtgat ataatgtaaa tacaatttaa gataactact cgaaggggag tagcttacac
    11581 gaaagtggta attaatcgtc aagacagtag aaatactcgg ttaattagca agggattcct
    11641 tgatagcaag accttcaaaa taagcattag gtatgtttgt tttggggtct ttttttattc
    11701 aaggaatttt tatttaaaaa aatttaaatt tcaagtatac ataagagccc ccaaacataa
    11761 aataaattta ttgaagagga gggcaacaaa tgtcttacaa aagtacttat caagaagtaa
    11821 ttaaaaattt agactcaagt ttatctggtt tgagtaaaga aagagcagaa gagttgttag
    11881 aaaaaaatgg agctaatgaa ttaaaagaag cagataaggt tcctacttat aagcttttcc
    11941 tagaaagttt taaagaccca ttagtaataa ttctattgat agctgcttta gtacaaatat
    12001 ttttaggtga aacagtggag tctataataa tttttgcagt tattattata aactctgttc
    12061 tgggtgttgt acaaacaaaa aaagcagaaa gttcattaga aagtttgaaa aatttatctg
    12121 caccaaacgc aaaggtaata agagacaata aaaaaatgac aattccagca aaaaatctag
    12181 tagttggaga tattgtattc ttagaagctg gcgattacat accagcagat ggaaggctta
    12241 tagaagcaca atctctcaag gttgtagaag ggatgttaac tggagaatca gaaccagttt
    12301 taaagcatac agaaaaaata agtgaagatg taagtttagg tgaccagaaa aatatggtat
    12361 ttagtggtgc tgtagttgtt tatggtagag gttcttttgt agtgactgga acaggtatgg
    12421 atactgaaat gggtaaaata gctgggcttt tagaaagtgc tgaaaataaa cttacaccat
    12481 tgcaacaaaa aatagaggat tttagtaaaa aacttggtgt aggtattgta atacttgctc
    12541 ttttaatatt tataattcaa gtagcaagaa attactttat ggctggaaat ggagaaatga
    12601 cacaagttat tttagattca ttcatgttct ctgtagcagt agcagtagca gctataccag
    12661 aagctctatc atcaatagtc actatagtat tggctgttgg aacaaatagt atggcaaaaa
    12721 aacaagccat aataagaaaa ttaccagcag tagaaacttt aggttcaaca agtgtaatat
    12781 gtactgataa aacaggtact cttacacaaa ataaaatgac agtagttgat tattatatgt
    12841 atggaactaa taatgatgag ttagataaag aaaaagtaaa ctattcttat catgctaaat
    12901 tattgactat ggtttctaca ttgtgtaatg actctcatat aactagtgat ggtaaagaag
    12961 ttggtgaccc aacagaagta gctttaataa attattcaag taaaaatgat ttagattatg
    13021 ataaattaag aagtaagtat atcagaataa atgaaatacc atttgactca gacagaaagc
    13081 ttatgtctac tgtaaatagt atatatggag attatataat gtttacaaaa ggtgctccag
    13141 atattgtgtt tagtagatgt aaatatgctt tgaaaaatgg ttcaaaagta gatataacag
    13201 atgagataat agaagaatat aaacataaaa atgaagagtt ttcaaataga gcacttagag
    13261 tattagcttt tgcaataaaa gatgtcccag aagaagattt tataccttca attgatgacg
    13321 aacatgacat gacattggtt ggaataatgg caatgataga cccaccaaga gaagaagtaa
    13381 tggatgcagt aaaagaggca aagagtgctg gtataaaaac tgttatgata actggtgacc
    13441 ataaaacaac agcagcagca attgcaaaag agattggtat aatggaagaa ggagacttgg
    13501 ctcttactgg aaaagagctt gatgctttga gtgaagaaca actatatgaa aaactagaaa
    13561 atataagtgt atatgccaga gtctcgccag aaaataaaat tcgtatagta aaagcatggc
    13621 aacataagaa taatataaca gcaatgactg gtgatggtgt taatgatgca cctgcattaa
    13681 aacaagcaga tataggaatc ggtatgggaa gtggtactga tgtagcaaaa gatgcttctg
    13741 ctatggtatt ggttgatgat aactttgcaa gtatagtaaa tgctgttgaa gttggtagaa
    13801 cagtatacag taatataata aaatcaatta cttatctatt tgcaggaaac ttaggagcta
    13861 tagtatccat agtatttgca gtgtttgcag gttggtcaaa tccatttaca gctttacaac
    13921 ttttgtttat aaacttagta aatgactctt taccagcaat tgcattaggg tttgaaaagt
    13981 cagaaaaaaa tataatgaat aaacctccta gacacccgga tgaaagtata ctagcaggag
    14041 gaacattgaa ggcagttgct ataagaggtt gtataatagg tgtagttaca ataattgcac
    14101 aatatatagg attacaagta tctccagaac ttggagtagc aatggcattt tcaacattga
    14161 tattagctag aataatgcaa acattacctg cacgttctaa taatcaaaca gtaataaaat
    14221 taggtttttt cagcaataaa tacgttatag gagcagtttt agtctgtctt gggctttact
    14281 ctttaacact aataccagtg ctaagaggaa tattctctat accaacaacg tttggattgt
    14341 atcagttggg gatttgccta gggttagcat taatttcatc aatattaatg gaacttaaca
    14401 aattaataaa tagaagtgaa gtaaaatagt caaaaaatta aagggcatat tgattttaac
    14461 aatatgcctt ttataaatag tctaatttat tctatattat cgattatttg gaatgctttt
    14521 ataacttcag gtaattcttt ttcttcaggc attctaaatc ttatgaaatt tttatcaagt
    14581 ccagtaaaat cacttccaga tataactctt atttttaatt tagcaaactc ttgttgtaaa
    14641 tctatgtcct tgtttttatg ttcaactgtc ataattgata cagtatcact agtttcagct
    14701 atattaaggt ttttccatgg aattaataat tgatttttta tatcttttgt tttttctttt
    14761 aattcttcta taaattgaac atcatctaat aaatttgctg caatactacg agatatttca
    14821 gacacttcgt aaggagtaga aatcttttta atagggctaa caagttgttc tggaagaact
    14881 gcatatcctg ctcttagtcc tgctaaacca aatccttttg aaaatgtttt taaagcaatt
    14941 acattatcat aattatttag aagcttgaca gctgaatttt cttttggcat gtattcacca
    15001 taagcttcat ctaccataac tgctatatca tatttagctg cttcctttac tatgttttca
    15061 attgatgata aaggtataat ttgtcctgta ggattgtttg gattgtctat gtaaattact
    15121 ttatattctg gatttatttt ttcaataaat tctttttcat taaacttgaa attatcctct
    15181 ttctttaaaa gaacataatc ataagttgca ccatgcattt ttatatctgt ctcatattct
    15241 gagaactgag gtgaatatcc taaaacttta tctccctttt caataaatag tctatttaaa
    15301 aggtaaataa catgtataga accatctgcc aaacaaattc tgtcagtatc taatgctata
    15361 aaatctttcc aataatcgac tatattatct tttaaagcaa ttgaatgagg atatcctctt
    15421 atcatatcaa attttaaagc attaaaagct tctacagctt tgtttgagta agacacggta
    15481 tttatacctt caccacagtc tatctcgaat acatcttctg aatgtccaga ttctattgca
    15541 taactttttt tcaactgcat ttcaatagct ttgttaattt ttagtttcat actttatcac
    15601 tccttattta gtcaataata aatgttataa aagttgctag atttaaaata ttatgaaaat
    15661 atataatagt aaaaatgaat ataaatatta t
//
LOCUS       DACXMZ010000077        15450 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.077, whole genome shotgun sequence.
ACCESSION   DACXMZ010000077 DACXMZ010000000
VERSION     DACXMZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.077"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(50..784)
                     /locus_tag="JD016_09550"
     CDS             complement(50..784)
                     /locus_tag="JD016_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAU4999878.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            910..1626
                     /locus_tag="JD016_09555"
     CDS             910..1626
                     /locus_tag="JD016_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAU4999879.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1760..2452
                     /locus_tag="JD016_09560"
     CDS             1760..2452
                     /locus_tag="JD016_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4999880.1"
                     /translation="MNNTILVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2430..3623
                     /locus_tag="JD016_09565"
     CDS             2430..3623
                     /locus_tag="JD016_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAU4999881.1"
                     /translation="MDIKLKNKSLLNHMFRPILDIIISCALTSMLILTSMYIIKSLYF
                     NSGISVINQLVYQLRDLRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTN
                     RKNQKLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRN
                     ITIEERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEK
                     SKNLNVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFN
                     EEKLIVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPV
                     LDLPNIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTMFEVQLP
                     IIQSA"
     gene            3905..4360
                     /locus_tag="JD016_09570"
     CDS             3905..4360
                     /locus_tag="JD016_09570"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAU4999882.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4350..5075
                     /locus_tag="JD016_09575"
     CDS             4350..5075
                     /locus_tag="JD016_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HAU4999883.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5047..7428
                     /locus_tag="JD016_09580"
     CDS             5047..7428
                     /locus_tag="JD016_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine methyltransferase"
                     /protein_id="HAU4999884.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREVQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7633..8862)
                     /locus_tag="JD016_09585"
     CDS             complement(7633..8862)
                     /locus_tag="JD016_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAU4999885.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            9463..10377
                     /locus_tag="JD016_09590"
     CDS             9463..10377
                     /locus_tag="JD016_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999886.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            10449..11036
                     /locus_tag="JD016_09595"
     CDS             10449..11036
                     /locus_tag="JD016_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HAU4999887.1"
                     /translation="MDKLMKICPTREIELQQKAKQLYEEEENRKIAYNAGLVEAEGYC
                     QYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHHGFEVFSVICKNGAMPK
                     STIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGLCVGHDTLAMKYMEAPI
                     TTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            11412..12128
                     /gene="pyrF"
                     /locus_tag="JD016_09600"
     CDS             11412..12128
                     /gene="pyrF"
                     /locus_tag="JD016_09600"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAU4999888.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            12154..13197
                     /gene="carA"
                     /locus_tag="JD016_09605"
     CDS             12154..13197
                     /gene="carA"
                     /locus_tag="JD016_09605"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAU4999889.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            13250..>15450
                     /gene="carB"
                     /locus_tag="JD016_09610"
     CDS             13250..>15450
                     /gene="carB"
                     /locus_tag="JD016_09610"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HAU4999890.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYL"
BASE COUNT         6220 a         1711 c         2778 g         4741 t
ORIGIN      
        1 ttatttacaa ttaatacaat cttgcttttt tatttaaaaa ttttaattat tatttaaaat
       61 tttttatctt ctctttatcc catataaaca atttctcaga attatttaaa gcaccaaata
      121 catttctttt aaaatcaggc atctctttat ttaaattagc aataagattt tttatgtact
      181 ctctcctagt atgaccattt gcagggcagg gatttgtgat aataggatag ttagaatctt
      241 ttgaaatttt cttagtcata tactcttcta tgtaaaccat aggtcttata actgttaaat
      301 cctgtctgtc taggtatgtc tttggggaaa aacaatttac tctaccctca taaaacatag
      361 acattaaaaa tgtctcaatt gcatcatctt tatgatgacc aagagctact ttattacatc
      421 caagtttttt agcattatcg ttcaaagcac ctcttcttaa attagcacac aatgcacaag
      481 gattcttttc ttgtcttata tcaaatacaa tttcttttat gtcagtttga aattcataaa
      541 aaggaacatt tatttcttta cacaatttat ctagtggaga attatcaaca cctccagtat
      601 ttaatgttat ggctatcaat tcaaagtttt gtggagaaaa tctttgataa cttttaagta
      661 tatgaagtag agttaaacta tcttttccac cagaaagtcc aaccgcaatt ttatcatttt
      721 cttgtatcat atcaaaatct tgtatagcct tacgagcttt acttagaagt ttttgtatag
      781 acattttatc accatccttt aaaaatatta aatttaagat ttatttaaga tttacttata
      841 aaatacttaa gtcttatact ctaaaattaa cattacagac aaacataaca aaaaggagaa
      901 gatttaatta tgaaaaaatt taaattaata ggagccatta tattagtagt atttataagt
      961 gtatttatat ctaaagatag attacttaag aaagataaag atccaatagt agctgctcaa
     1021 catgattcag aacaagatgt tgaggcaaat tctcaaaata aaaatcactc aaatagtata
     1081 tcaaatatat tattggtaaa caagacaaat ggaattagca aaaactatac tcctgaaaat
     1141 attactaagg taaacatacc ttttgttgaa gaagcaacag aggaagaaaa acaaatggca
     1201 ggagaacctg caaaggcagt tgaagattta gttaagcaag ctaattcaga aggaatacag
     1261 tttttagggt cttcagcata cagatcttat gatactcaac tggatactta tacaagaagg
     1321 gtaaaatctc agggacgaga aaaggcagat gcatatgtag caaagcctgg ttacagtgag
     1381 caccaaactg gactttgtat tgatttaaca aatccagaaa gatggtttgt tggaagtaca
     1441 aaagaagcta aatggttggc agaaaatgct cacaagtttg gatttataat aagatatcct
     1501 gaaggtaaag aagatataac aggtacagca tatgaaccat ggcatattag atatgttggt
     1561 aaggatgctg ccgaggaaat ttattcaaaa ggattaactt tagaagaata tttacaaaat
     1621 aaataatata aatgtgtaaa taatttgtat tatattggaa atttagaaat taataaattt
     1681 atagattaca ctaactaaaa tatagtataa ttttatatga ataaagctat atacaaagat
     1741 taagttagga tgtgtaaaaa tgaataatac aattcttgtt gtggatgatg aaaaggaaat
     1801 aagagacctt atagagatat atcttttaaa tagtggatat aatgtaatta aagctgaaga
     1861 tggaaaagag gctctagata ttttaaaaaa ggaaaatatt catttaatga tattggatat
     1921 aatgatgcct aaaatggatg ggatagaagt ttgtagaaaa gttagagaaa gtttgaatat
     1981 accaatactt atgttaagtg caaaatcaga agacatggat aaaatacaag ggataatgac
     2041 tggtgcagat gactatttga caaaaccatt taacccactt gaacttactg tgagagtgaa
     2101 gtcgctgatt agaagagcat attattttag tggtgctaat aatgaagata aagatgtcat
     2161 aaaggttgga gtagtaatta tagataagga aaaacatagt gtaacagtgg ctggaaatga
     2221 aataattttg acatcaagag aatttgatat attatattta ctagcaaata ataaaggcag
     2281 agtatatagt acagaagaaa tatttgaaaa ggtatggaaa gagaaatatt accaatctaa
     2341 taatactgta atggttcata tgagcagaat aagagataaa attgaaaaat atacagatgg
     2401 agaaaaaata attcatactg tatggggagt tggatataaa attgaaaaat aaaagtttat
     2461 taaatcatat gtttcgaccc atattagata ttataataag ctgtgcttta acttcaatgc
     2521 ttattttaac aagtatgtat ataataaaat cattatattt caatagtggg atatctgtaa
     2581 ttaatcaact tgtatatcag ttaagagatc tcagaagtct tataggagag agtttatttt
     2641 atccattttt atttacaatc acatcattag caatttattc catgatatcg tacaagagaa
     2701 ataagcaaat tgtagatttt ataagaaaga ctaatagaaa aaatcaaaaa ttagaaatca
     2761 ttaaaagtgc tgtaaattta atggatgaag gtaacttaga aaaaagtata gatattgagt
     2821 atgatttaga tatattaaag gataaagaaa aggatgatat tgatgaacta gcacataaaa
     2881 tcaataatat agtccatcaa cttagaaata taactattga agagagacaa gctcagcaga
     2941 caaaaacaga tttaataaca aatgtgtcac atgacttaag gacacctttg acatccataa
     3001 tgggttattt aggagtgata gaagaaggta agtataaaga tgaagttcag atgatgtatt
     3061 atgttgatat tgcatatgaa aaatcgaaaa atttaaatgt tcttataaat gatttgtttg
     3121 aattgactaa aatgcaaaat aatacaataa aattaaacaa aatagaaata aatttagtag
     3181 agttattaag tcaagtagta tctcaatttg aaatttattt taaacaagaa tttatggtta
     3241 gtagaattaa ttttaatgaa gaaaaattaa ttgtaaaagc tgatccaaat aaattggtaa
     3301 gagcatttga aaatcttata actaatgctg tgaaatatgg aaaagatggg cattatgtag
     3361 atatagtcac agagaaaaag gaagatatgg cggttgtaaa agtgataaat tatggggaac
     3421 caattccagt gttagattta ccaaatatat ttgatagatt ttatagagtt gaaaaatcga
     3481 gaaatagaaa tgttggaggt tctggattgg gtcttgcgat tacaaaaaat attgtaaatt
     3541 tacatgatgg agaaattact gtttcaagcg ataaatatca aactatgttt gaagtacaac
     3601 tgccaataat acaatcagct tagttataaa ataaaagtac gattatgtga tataaatatt
     3661 ataacaataa agtgtttaca aaaataaaaa agtaatatat aatttaagtc agtttaataa
     3721 gatacttatc aagagttagg ggagagagtt agctcagtga tcctatacca accttttatc
     3781 aggtggtcct gctaacaacg atgagatgac ttgaaataat atactcttct tatcaataat
     3841 agataggaag agtttttttt agaattaatg taacaaattt taaagtataa tggaggatta
     3901 aataatggaa aaagtagaaa gttttaagct agaccataca aaagtaaaag ctccttttgt
     3961 aagaaagtgt agtgtacttg atggagtaaa gggagataaa gtaactaaat ttgacttgag
     4021 atttttacag ccaaatgtag aaagttttgg aactgctgct atgcacggtt tagagcatct
     4081 tttagctact tatttaagag atacattaga tggtgtaata gacttatctc caatgggatg
     4141 tagaacagga ttttatttaa tactatgggg agatgtagat gccaaaacag ttaagattgg
     4201 tttagaagaa gcattaaaaa aagttcttga atcagataaa atgccagcag ctacagctat
     4261 agaatgtgga aactacagag acttatcttt atttggggca aaggaatatg caaaagatgt
     4321 tttagataaa ggattttctt taaatatata tggagaataa tttaattgga agagaagata
     4381 taagcataaa tcaaagtgaa gttttaagat atttacaata cagaggtcaa gagatagata
     4441 aagaccttaa atatactatt gatgaatgtg tagctataac taaaaataaa ataaatccta
     4501 gatatttatc taggatttat cctattaaaa taaataaaga taatgttgta gaattagagg
     4561 gaacaaattt aacattagag agtaaagatg tatatgaact tttaaaagaa tgtaatgagt
     4621 gtgtaattat ggcagctact atagggatag atatagaaaa agaaataaga cgacactctt
     4681 attcaaattt aacaaaagga ctaataattg attcatgtgg aacaacagca atagaagaag
     4741 tgtgtgacat tgttcaaaat aatttagaaa aggttttttt aaagcaagat aaatttataa
     4801 ctatgcgata tagcccagga tatggagatt taccaataga aaaaaatata gatatattaa
     4861 acgttttgaa tgctcaaaaa caaataggtc taacaattac ttctaatgga attatgatac
     4921 cgagaaagtc agtagtggct attataggta tttctaatac aagaataaat aaatctaaga
     4981 aagaaaagtc gtgtgaaaat tgtaaaaact atagaaactg tacatataga aaaggagcga
     5041 actcttgtgg aaataagaca atatctgaaa aataatatat taatatttga tggtgcaatg
     5101 ggaacaatgc ttcaacaaaa agggttaaag ttaggtgaaa atccagaagt atttggtctt
     5161 caaaatccag ataaacttat agaaatacat acagcatact tagaggcagg ttcgaatgta
     5221 attcttacaa atacttttgg atgtaatgaa ctaaaattag atagtaagta tactgtagaa
     5281 gaagtgattg acaatgctgt tttggtggct agaaaagcta tagaaaatgt agataatact
     5341 aaaccaagat atgtagcttt agatattggt cctataggag aaatgctaga gcctatgggt
     5401 actttaagtt ttgataaagc atatgaaata tttaaaagac aagtactaca aggcgttaaa
     5461 tctggagttg atgtaatagt aatagagact atgatggatt tatatgaagc taaagtagct
     5521 gtattagcag caaaagaaaa ctctgattta cctattttct gtactatgac atttgatgaa
     5581 ggtggaagaa gttttactgg atgtatgcca gaatgtatgg tggctactat agaaggtcta
     5641 ggtgtagatg caataggagt taattgttca ttaggaccta aacaactttt accaatagtt
     5701 gaaaaaatag catcaagagc tacagtccct gttatggttc aagcaaatgc aggtcttcca
     5761 aatatagttg atggagaggc tatatatgat gttgatgcaa aagaattttt tgaaggggtt
     5821 aaaaagttcg ttgaagttgg tgctactata ataggtggat gttgtggtac gaatccaagt
     5881 tttataaaag aaatatctga gaatataaat tctgttacaa agggatgtat cgagaaaatt
     5941 gataaatgtg tagtctgttc accatcaaaa tttgtggaag tacaatcgcc tacagttgtt
     6001 ggagaaagat tgaatcccac aggtagaaaa tctctacaag aagcattaaa aaatgaaaat
     6061 gttgattatg ctataaattt aggtttagaa caagtaaatg ctggagcaca aatacttgga
     6121 gtaaatgttg gtcttccaga gattgatgaa aaaaaactaa tgccaaaatt aataagagag
     6181 gttcaagcag tagtagatac acctttacaa gttgattctt ccaatgtaga ggccttagag
     6241 caaggactta gatattataa tggtagaaca atagtaaact ctgtaaatgg caaagaggag
     6301 tctttagaaa gtatattacc aatagttaaa aagtatggtt catgtgtggt tggacttaca
     6361 cttgatgaaa aggggatacc taaaaaggct gaagaaagat ttgaaatagc aaaaaggata
     6421 gttaatagag cagtttctta tgggataaaa ccaaaagata tatttataga ctgtttatca
     6481 ttaactgttt cagcacaaca agaagaagca atagaaacta taaaagctat aacaatggta
     6541 aagacactag gagtaaaaac tatattaggt gtttcaaata tttcatttgg tcttccaaat
     6601 aggaaagcac ttaatgcttc ttttctaaca ttagctttag gggcagggct tgatttagca
     6661 attataaatc caaatgaata tagtatgatg gaagctatca attcttttaa aatattaaat
     6721 aacacagata aaggatgtat aaactatata aatcaatata gcaatataaa taattcaaaa
     6781 aaatcggatt catctacaaa gattgataaa gatttgccct tggatatttt agttgaaaga
     6841 ggattaaaag atgaagcaaa aaatgtaaca ctgaatcttt taaaagagaa ggatgaaaat
     6901 tatatattag atgagatttt aattcctgct cttgataaag taggaaaaag atatgatagt
     6961 ggagatatat ttttaccaca attgattcaa tcagcagaaa cggtaaaagt ttcattaaat
     7021 acaataaagg aaactttatt aagtaaaagt agcaataatg tatccaaagg aaaaataata
     7081 gttgctacag taaaaggtga tatccatgat attgggaaaa atatagtaaa aataatgcta
     7141 gaaaattatg gatatgaagt tatagattta ggtaaagatg tccctattga agaagtagtg
     7201 gaagttgcta aaaagagaaa tataaaatta gtaggattaa gtgcacttat gactacgact
     7261 gttcaaagta tgaaagatac aattcaggca ctgagagata atgagataaa tgcaaaggta
     7321 tttgtaggag gtgcagtttt gacagaagaa tacgctgaag aaatgggtgc tgattattat
     7381 tcaaaggatg caaagtctgc tgttgaaata gcaaagttaa atttttagca tataaattaa
     7441 aataaaggtt ttactaaata ggatatttta tatataatat aaatataagt tttagatact
     7501 tatgtttaaa acaataggaa ttaatctata ataatgagat taattaatat aaaaaatatt
     7561 tatcatcaac attaaaaagg gctgggaagt ctggtataac ttccatcagc cctttttagt
     7621 taaaatcata tgttaaaaag cccaattacc atttttaaaa atctgtattt gctttccatc
     7681 ataagtctca ccaataatat ccatatcagc agaaccaatc ataaaatcta catgtgttaa
     7741 actgtcattt acaccatgtt catccaattc ttcatctttt aaattatttc caccttccag
     7801 acaagtctta taagcagaac caattgcaaa gtggcaagaa gcattttcat catatagagt
     7861 attataaaaa attatatccg tattggatat tggtgagttg tatggaacaa gagcgacttc
     7921 tcctaaataa tgagagcctt catccgtttc aataagtttt tctagtgtat ctagtccctt
     7981 ttcagcagag aaatctatta ttttaccatc tttaaatgtt aagaagaaat tgtcaattat
     8041 gtttccacca taaacaagag gttttgtact ataaacagtt ccatttactt taaacttatg
     8101 tggcattcca aaaatctctt cagttggaat attcggatta aaacttattc cattagggtc
     8161 tttggatgct ccacttagcc aaacatgacc ctttggcaag tctagagtca aatcagtttt
     8221 tgaggattta tattttaaag ttttaaagtt atttttattt agaaagtcca ttttagattt
     8281 aagattttca ttatgttctt cccatgcttt tatagggtcc tgaccatcta ctcttgaaca
     8341 tttaaatata gcatcccaca gtttttctat tgctttatct tctgataaat ttggaaacac
     8401 tttttttgcc caatcttttg ttggcacaga gactatactc catttacatt tatcagttag
     8461 agtgtaagaa cgccattctt ttaaagcttt tccagatgcc ttctgaaaat ttgcaactct
     8521 ttcagggtca acatctttta ataaatctgg attttgggca tagactgata aaaatgcccc
     8581 accttctttt gctatatcta catattgttc tgctgtccat ttggggaact catgaaatgc
     8641 ttcatctgga gcatataagt atttaataag agtactttct tcatcagacc actctatata
     8701 aacattttta gcaccacatt tatatgcttc tttaagtgca tttcttgcaa aatcagcaca
     8761 ttctattggt gattttataa gtagaatttg acctggctgt atgttaactc caacttttat
     8821 tgctaattca gcgtattttt ctaaatttct atttaaattc atatttatcc tcctatattt
     8881 ttaatattga ataacttata aagtattata acattactta tgtttttaat attgaacaac
     8941 ttataaaata ttataacttt acttagattt tgggtcatat tcaaaatatt tatactttat
     9001 agattgagaa ataaaaaaaa tattttaatt aaaacaaatt atgaggataa caaacttatt
     9061 taagcagtat gttttttgtt gataataata ttaaagtaga aataaaaata cttttttagt
     9121 gaaaagctat acaagaatac cgaaaaaaac ttataatata ataatgagat agatatttat
     9181 aaatgaaaaa aaatttttct ggcaaagctt aattaaaaag gtaatagcta gtgttgttat
     9241 ttaaattagc atagaaaatc aaataaaaaa attatgctaa tgtatagaca ttgataaggt
     9301 agaatttaag ccttttgaat gctatattaa actagagtag atattcttat gaatttcaaa
     9361 atagggcagg aggaaaaagg aaataaaact ataaaaaata taaagtattt atataaattt
     9421 atagagttta tacgtcagaa gatttcagtt aggagggcat aaatggagct ttatgaagcc
     9481 attttttata gaaaatctgt aagaaatttt tccagtaaga agataaaaaa gccattgatg
     9541 gaagaaataa aaagaagttg taaaaattta gaatacttaa atgaagattt aaatattaaa
     9601 gcacatgttg ttgatagagg tcatataata cattttttga tgggtaagga atgtaaagta
     9661 aaggcacctc attatatagt agttacatca gacaaaggtg aggactattt acagaatata
     9721 ggatttgcta cagaaggtgt agttttgcgc cttacaacat tgggaatagc tacttgctgg
     9781 ttggaaggaa acttaaagag agaagatatc ttggaatttg tagatattgg gaataagaat
     9841 tttgaagaag atgaggaaga tgatttagat tatctagctg gaataaaaac agaagaagat
     9901 gatgaaaaca aaatagaaaa tccttatata ataatagcat ttggatatcc agcaaaaaat
     9961 gaagagttgt ttaggacgag atatgctaga cctgatagaa agccagtaaa agatatgata
    10021 aataagataa ataggaatag aaagtggaaa gatatcttag aattgacaag aagagctcca
    10081 tctgtaaaaa attctcaacc gtggtatttc cataaggatg aacgaggtct tcatttattt
    10141 gaaaagagac ctaagaaaca ctgtgaagat atgaataagg ttagcttagg tgtagcctta
    10201 agacattttg atatagcttg tataaaaaat aaaatagatg tttcatatga aaaacttcct
    10261 ataaggaata aaataggaaa atcatatttt ataactgtag tcgagcatgt gaaaccagaa
    10321 gaagaaaccc aagaagaaaa tgtaactcta gaaaaagaag aatcgcaaga tgaataaaat
    10381 tttggaaggg gatattggat atgtataagt gtggaaaatg taatcttaga ggctgttcta
    10441 atggaaatat ggataaatta atgaagatat gtccaactag agaaattgaa ctacaacaaa
    10501 aagcaaagca actttatgaa gaagaagaga atagaaaaat agcttataat gctggattag
    10561 ttgaagcaga ggggtattgt caatatacta gactaaaaga gattatagag tttgctaaaa
    10621 agtgtggtta taaaaagatt ggcatagcat tttgtattgg actaaaaaga gaaatgcaaa
    10681 cagtgcaaaa aatatttgaa catcatgggt ttgaagtatt ttctgttata tgtaaaaatg
    10741 gggcaatgcc taagagtaca attggactag aagcatctca aacaatatca aagtgttcag
    10801 atgaggtaat gtgcaatcca ataggacaag ctttattttt aaatgaagct aaggttgacc
    10861 ttaatgtaat tcttggatta tgtgttggtc acgatacttt agccatgaaa tatatggaag
    10921 ctcctataac aactttagtt gtcaaagata gagtaactgg gcataatcca gttgctgcaa
    10981 tatataatgc agaatcatat tttgagaaaa aattgtttga taaagatgaa gaataattca
    11041 aagacttatt gacataaaaa gcaattagtg gtttaattat aaaaaagata aaactttaaa
    11101 ttatcctata gatacataaa gttatataaa taaagaaaaa tattacagct aaattcctta
    11161 aagtagtcca gagagactaa agggttatag ataatgttag tatataacct ctagtaaatc
    11221 ttctagaggt ttttgatgag tataaaataa aaatttaaat ataataaagt cttttaaact
    11281 agtccagaga gactgggaaa gatatctcat agctaatgaa tagagttatg tatagataaa
    11341 atccttatgc ctcttttggt gtgaggattt ttttatgatt atttaggctt tattaaaaag
    11401 gagatgtaaa aatgataaat ggtaaagaaa aattgatagt tgcaattgat actgacgaat
    11461 ttgataaagc taaagaacta atagacagat tagaagatag tgtagacata tttaaagtag
    11521 ggctagagca atacgttgcc acaaaaggta aaacaataga ttatcttaaa gaaaaaggta
    11581 aaaaaatatt tttagacctt aaattccatg atataccaaa tactatgaaa agtgcagtga
    11641 aagctgcagt gagagataat gtttggttaa tgacaataca tgtatcagat atggaaggaa
    11701 tgagacaatg tgcactaatt gctagggaag aagcagaaag attaaatata gaaaagccat
    11761 tagtagttgg agtaacagtt ttaacttcat taagcaacca agatttacag gacataggat
    11821 gtaatatgac aacagaagag ttagctataa agagagctaa actggcaaaa gaatcaggaa
    11881 tagatggggt tgtttgttca gcgcaagaag tagataaaat agtagaagca tgtggaactg
    11941 attttataac agtttgccca ggaataagac ctaaatcagc agaggttgga gaccaaaaaa
    12001 gagttgtaac tccatcagat gcaataaaaa aaggtgctca ttatttagtt gttggaagac
    12061 ctataacaaa agcagaaaat ccaagagaat cagctattaa tattgtaaga gaaatagaaa
    12121 atgcttagat aaaatatttg ggaggtagct tacatgaagg ggaaattaat tttagaagat
    12181 ggaacagtat ttgaaggaaa agcatttgga tatctaaagg aagctattgg agaagttgta
    12241 tttaatactt caatggctgg gtatggagaa ctattgacag atgcaactta ttatggacaa
    12301 atagttacta tgacttatcc acttatagga aactatggaa taaatttaga agatgttgaa
    12361 tcaaataagg tgcaagtaaa aggacttatt gtaagagaaa aaagtgataa cccaaataat
    12421 tttagatgtg aaatggatat agacacatat ctaaagcaaa ataaggtaat aggacttgaa
    12481 ggaatagata caagagcatt aactaaaatt attagaaaca atggaactaa gagaggaatt
    12541 ataactttag aaaatgtatc attacaagat gttcagtcta aattagaaga gcattgtaac
    12601 aaagattcag taaaaaatgt tacaagaaaa gaaattgaaa atataaaagg tactggagct
    12661 aaagttgtag ttatagactt tggtgttaaa aatagtgtac tagagtctct taaggcttgt
    12721 aactgtgata taactatagt accagcaatg actacagtag aagaaatttt aaatataaat
    12781 cctgacttaa tcttattatc taatggccca ggaaatccag aggatataaa tgatatagtt
    12841 gaaaatataa aaaaattaat aggcaaaaag ccattgacag gaataggttt aggacatcaa
    12901 attttagcat tggcattagg aggaaagact tcaaagcttg tatttggaca tagaggaggt
    12961 aatcacccag ttaaatgttt agaaactgga aaggtattta taacttctca aaatcatgga
    13021 tataatgttt cagaaatgcc agaaaataca gaagtaactc acataaattt aaatgacaat
    13081 actatagaag gaatgagaca taaggaatta ccaatatata gtgttcagta cattccagaa
    13141 tacacaacaa aagaagattt agattgtata tttaataaat tcttagcatt aatatagatt
    13201 tagtattatt tacagatagt aaaaaccatt tagataagga gaaaaattta tgcctaaatt
    13261 agatagtata aagaaaactt tagtattagg gtcaggacca ataataatag gtcaagctgc
    13321 agagtttgac tattcaggaa cacaagcttg ccagtctttg aaagaggaag ggattgaagt
    13381 tgtattaata aatagtaatc cagccactat aatgactgat aaagaagtgg cagataaaat
    13441 atatatagaa ccattaacaa tagaatttat agaaaaaata atagaaaaag aaagaccaga
    13501 cagtttactt gcaggtatgg gtggacaaac agggctaaac ctagcagttg aactttatga
    13561 aaaaggcata ctagataaat atggagtaaa gataataggt acttcagtag aatctatcaa
    13621 aaaaggtgaa gatagagata tatttagaga agtaatgaaa gaaataaatc agcctgtaat
    13681 agttagtgat atagttacag atttacaagc aggtcttgat tatgcactta caattggata
    13741 tccagtagtt gtaagaccag catacacact aggtggaact ggtgggggta tagctgataa
    13801 tgaggaagaa ttaagagaaa tactttcaca tggtctacaa ttaagtcctg ttggacaagt
    13861 attaattgaa aagagtataa aaggttggaa agaaatagaa tatgaggtaa taagagattc
    13921 aaagggaaat tgtatagttg tatgtaatat ggaaaatata gacccagtcg gagttcatac
    13981 aggagacagt atagttgttg ctccaactca aactttaagt aacaaagaat gtgcaatgct
    14041 taaaaaagca tctttagata tattaaatgc agtagaagtt caaggtggtt gtaacgttca
    14101 atttgcactt aatccacata gttttgaata tgcagttata gagataaatc caagagttag
    14161 tagatcatca gctttagcat caaaagctac aggatatcca atagctaaag tttcatcaaa
    14221 aatagcttta ggttatactt tagatgagat agaaaatgca gtaactaaaa aaacttatgc
    14281 ttgttttgaa ccaactatag attatgtggt ggcaaaaata ccaaaatggc cttttgataa
    14341 atttaaaaaa gcaaatagaa aattaggaac taagatgatg gcaactggtg aaattatgag
    14401 tataggaagt aattttgagg ctgctattct taaagggata agatcacttg aaacaggaaa
    14461 atattcatta gttcacacac catctgagga gagaagcatt gaggaattaa agaaaagagt
    14521 tgtagtacct gatgatgaga gactatttga tttagctgaa atgataagaa gaggctataa
    14581 agtagaaatg atagaacaaa ttacaggtat agataaatgg tttataaata aatttaaatg
    14641 gatagtagag caagaagaaa aattaaaagt tatgaaaatt gaagatttaa ctaaagacta
    14701 tcttcttgaa ttaaagaaaa agggattctc tgataaaggt atatctgacc taatgaagat
    14761 aagtccagaa aaattatatg aattgagaag tctatacaat ataaaaccag catataaaat
    14821 ggttgatact tgtgcaggtg agatagatgc tatatctcct tactattatt caacttatga
    14881 gcaatatgat gaggtagttg ttagtgataa gaaaaaggtg atagtattag gttctggtcc
    14941 aataagaata ggtcaaggta tagaatttga ctattgttca gttcactgtg taaaatcttt
    15001 aagaaaaatg gacatagaaa ctataatcat aaacaataac cctgaaactg taagtacaga
    15061 ctttgataca tcagataagc tgtattttga accattaaca gaagaagaag ttttaagtat
    15121 aatagaaaaa gaaaagccag aaggtgtaat actacagttt ggtggacaaa cagctataaa
    15181 attggctaag tttttacatg aaaaaaatat acctatactg ggaacagatt ttgatgatat
    15241 agatgcagca gaagatagag aaaaatttga tgatttacta gagagactag atataaatag
    15301 accaaaagga aagggtgtat ggtcacttaa cgaaggtatt gaagttgcta atgagttagg
    15361 ttatccagta ttagttagac catcttatgt acttggtggt caaggtatgg aaataactta
    15421 taacgaagaa aaattatctc aatacctaca
//
LOCUS       DACXMZ010000078        15412 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.078, whole genome shotgun sequence.
ACCESSION   DACXMZ010000078 DACXMZ010000000
VERSION     DACXMZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.078"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..840
                     /locus_tag="JD016_09615"
     CDS             <1..840
                     /locus_tag="JD016_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAU4999891.1"
                     /translation="ARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRAERKNTASKE
                     NRGNTTSSMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKS
                     SVSDFKRGMVQEQQSRQTGRLEKQTQHRQNIADKRMELQKAQEARQAQRKADGSATTG
                     ATRPHERPATASTIPKPSMEKTQEVKRPATATTSKTSEPVKTSVIKERPLSSGASDRK
                     ATQSVQPVHRQNVEKVVSQETRQNYKAERTTKTQTFEQSKRTTEHTKKNRNLVTKKGQ
                     KKK"
     gene            837..1844
                     /locus_tag="JD016_09620"
     CDS             837..1844
                     /locus_tag="JD016_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAU4999892.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTKSSIKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVAHFDNATAQAIM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGILVSPTQMYHAGDPIGYADLSSSYWQQHLIGAGRVK
                     Q"
     gene            1860..2771
                     /locus_tag="JD016_09625"
     CDS             1860..2771
                     /locus_tag="JD016_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU4999893.1"
                     /translation="MFKKNKKQTETIKEPKEKKVRTVKVGTHKKTVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWNNSKEAIEARTQA
                     ISGYLTKELQDLNVDTIKTDIPTSSTVTDVIVWHIEQSGTDTFSATYEVDQQIKEGEQ
                     TSNVKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDYEPKTPEADNSVDADTVNDATA
                     FLETFFKLYPTATEKELAYYVAGNVLEPIGRDCLYSELVNPIFTKDGDNVKVKFAVKS
                     LDNQTKATQVSQYELVLHKDGNWKIIG"
     gene            2860..3060
                     /locus_tag="JD016_09630"
     CDS             2860..3060
                     /locus_tag="JD016_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999894.1"
                     /translation="MIKNRIKEFRARYDMKQEDLAKAVGVRRETIGNLEKGRYNPSLV
                     LAWNIAKTFHVTIEEVFTVIDD"
     gene            3077..3301
                     /locus_tag="JD016_09635"
     CDS             3077..3301
                     /locus_tag="JD016_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999895.1"
                     /translation="MLGLKKRDIKKALKAGAKAGGVSLADVRADIEATIDEAMNSTDP
                     EVQANFKKYFGNKRPTPEEYIYKITKKTKV"
     gene            3363..4061
                     /locus_tag="JD016_09640"
     CDS             3363..4061
                     /locus_tag="JD016_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4999896.1"
                     /translation="MGSVLIIDDDKELCTLMKKCIEQEEMSALVANGGTEGLRLIDEN
                     KNLFSLVILDVMMPDIDGFQVLEKIRETSNIPVLMLTAKSGEDDKVFGLRLGADDYLT
                     KPFSIKELMARVNSLIRRYTTLNPTFAEEDCITLKGMKIDKVNRTVLVNNISVELTSK
                     EFDLLTFLASNKGRIFTKKQIYTQVWKDDYAFDDNNIMSFISKLRKKIEADPEHPFYI
                     LTIRGVGYRFNKEA"
     gene            4064..4981
                     /locus_tag="JD016_09645"
     CDS             4064..4981
                     /locus_tag="JD016_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU4999897.1"
                     /translation="MNTYLLIAFVLALVIIACLVYKLYHINEQISFIKEVLVDIKSGN
                     LNRRILVQENDITKQICYDINQIAINNQSQLIHQKQSEQAYKRLMTSLSHDVKTPLAS
                     LVGYLEAVESQIVIGKEKDEYIHVASEKAQHLKHFVEHLFEWVKLDSGEQIFHFEILD
                     LNELTRNIMTDWILILENSYFEYEVEIPEEEYQVRIDINAYTRIINNLLQNIIVHSEG
                     NKMMLRVLKDQQRIKVIITDNGKGIASDKLPHIFERLYQCDNSRSTKGNGLGLAITKE
                     LVNAHKGTITANSILNHGTEFILSFPKAL"
     gene            5133..6056
                     /locus_tag="JD016_09650"
     CDS             5133..6056
                     /locus_tag="JD016_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4999898.1"
                     /translation="MSDTVIKTKQLTKKYGKQTVVNKVNLNVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTITTSGNVEVFGKNIDGNEKRIYSRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTASPNAVKHALEVVGLPYKDKKTFAKYSLGMKQRLGIANAILHDPELLILDEP
                     TNGLDPIGIAEVRDFIKKLSVEHGKTILISSHILSEIAVLADDIGIIDKGVLLEESSM
                     SELEKRNRKYISLQVSDIPKTILILERQFHVKDYSVQDEQTVQIYDTTLNMGEVNKAL
                     VMEDITVISSGLCNDTLEDYFKKITGGEGIA"
     gene            6049..6783
                     /locus_tag="JD016_09655"
     CDS             6049..6783
                     /locus_tag="JD016_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4999899.1"
                     /translation="MLKLVKLEFCKLRRKKLIWLMLLSAFVMPFFAFLYFGYLGQTNI
                     DPRLFYKWSAFGCTLFIILPFILGLFSTMLMHDENRYDMLKQLWIVPVSKMGYFFSKF
                     FVVLIYSVCFMLITAFASVLFSVIPNYVVFEWSSVLFLLERCLEIAVLTSFAILPILA
                     ISSSQKGYIFPLCITLIYVFAGFLITPINSCLHPLSCISVIIARNEAILGLSLPQNNV
                     PLALLSICVWGIASTLLAKIKLSTRK"
     gene            6793..7542
                     /locus_tag="JD016_09660"
     CDS             6793..7542
                     /locus_tag="JD016_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4999900.1"
                     /translation="MRILLWAEFQKLRRSNIIMFTIFSTILIAVIVLISGVTTVSEEQ
                     LTTAMAGWYMTITQVWATMFVLPAVIALLGSYMICREEQDDTLKSLRLIPVNETKLTT
                     AKMIVALVFSMLVYLLLFMITFVTEAILHFSDLSMAMFSEFLKMYLLEGIGVFFAVSP
                     IIAIVPYLKKSYWLALLLAEIYSFAGLFMSMSNTLKTFYPITAIFGISGYYETTIQDY
                     FCSLGILIFCGCISAILLKNLNHRQRKKANE"
     gene            7535..7726
                     /locus_tag="JD016_09665"
     CDS             7535..7726
                     /locus_tag="JD016_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAU4999901.1"
                     /translation="MNKLCRIRKGKIFFGVCGGLAKCFNVDVKIVRLLFTITGFIFPK
                     SIIAYIILMIVIPKEPKTR"
     gene            complement(7847..8206)
                     /locus_tag="JD016_09670"
     CDS             complement(7847..8206)
                     /locus_tag="JD016_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999902.1"
                     /translation="MRKNKETQTFDFRPLGLAIREAREKAGLSRNDLGDKVFYGERHI
                     ADIENIGSHPSFQLFHDLVTMFNISVDEYFYPSEKVEKSTARRQIETSLDLLSDNELK
                     IIQGTIDGILNSRESKK"
     gene            8438..8617
                     /locus_tag="JD016_09675"
     CDS             8438..8617
                     /locus_tag="JD016_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999903.1"
                     /translation="MITYRRTLRRSQLDNYFCLVLQSSYPLHELKIHIDSIYITVCLC
                     SYIQMGCFYFIEIIF"
     gene            8717..9136
                     /locus_tag="JD016_09680"
     CDS             8717..9136
                     /locus_tag="JD016_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAU4999904.1"
                     /translation="MKTSSFKQAIEAQFDCLSKKVIKRAVKKGYRDMKRREKRECPFS
                     DIPDYEQERFGTFDKYESDYTVFNILGIEVWVEDDQLSEALKTLTEKKRNIILLSYFM
                     DMSDSEISEFIKIPRSNVQYHRTKTLETMKKIMEERK"
     gene            9133..9366
                     /locus_tag="JD016_09685"
     CDS             9133..9366
                     /locus_tag="JD016_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU4999905.1"
                     /translation="MKKHEHLPFEVILSATEGEPEAVETVLKFYDGYISKLCLRKLYD
                     EYGNVCMVVDADLKNRVQTALLDMLMNFEIVVM"
     gene            complement(9439..9648)
                     /locus_tag="JD016_09690"
     CDS             complement(9439..9648)
                     /locus_tag="JD016_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAU4999906.1"
                     /translation="MRTYHYSVRVGRGKATFPTVALGSLRGSLAFFWKGRGVQSPWLA
                     TSQTYLSALFNFQRTIGYSAYQNQF"
     gene            9758..9961
                     /locus_tag="JD016_09695"
     CDS             9758..9961
                     /locus_tag="JD016_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAU4999907.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENLDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            10040..11230
                     /locus_tag="JD016_09700"
     CDS             10040..11230
                     /locus_tag="JD016_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAU4999908.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYSWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLNDGIDTVGGKMTVCQLYEKKNSQRKNIKRA
                     TEKGRLYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSEKGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEQKVILTPEQEERLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLKDSEIGYYIETPKTKNGKRQLPLTE
                     RAYQAIQRILKNREKAQPLIVGGYSNFLFLNREGLPKVAGNYEGMVRGLIKKYNKYHT
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            11270..11464
                     /locus_tag="JD016_09705"
     CDS             11270..11464
                     /locus_tag="JD016_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999909.1"
                     /translation="MHFHAGKCQLMQGIFQKENTDKALKYGIYRHLPIYEKIIRNLVS
                     FLTKNRYRYVLTMIIIIDKL"
     gene            11522..12475
                     /locus_tag="JD016_09710"
     CDS             11522..12475
                     /locus_tag="JD016_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAU4999910.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            12465..13421
                     /locus_tag="JD016_09715"
     CDS             12465..13421
                     /locus_tag="JD016_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAU4999911.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFVIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMMFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            13418..14173
                     /locus_tag="JD016_09720"
     CDS             13418..14173
                     /locus_tag="JD016_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAU4999912.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSIQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVKKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            14265..15230
                     /locus_tag="JD016_09725"
     CDS             14265..15230
                     /locus_tag="JD016_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAU4999913.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         5489 a         2281 c         3080 g         4562 t
ORIGIN      
        1 gctaggcgta tggaacaccg tattgcaagg gcggtaagtg ctggcggtat ttcgggcggt
       61 gtggctggtg cggtagctgg aagtgccgtt gctggaaaac gagctgaaag aaaaaataca
      121 gcttctaaag aaaatcgagg caataccact tccagcatgg gacagcgtgc aggctcaaag
      181 gttggtgctg tcttagatac gaaaaataaa gtaaaagaca aggcaaacgc tgtcaaagaa
      241 aacatcaagg atatgccaac acagaccgct tatgcggtat attccgcaaa ggaaaaagca
      301 aagtccagcg tgtctgactt caagcgtggc atggtgcagg aacagcagtc cagacaaacg
      361 ggacgattgg aaaagcagac acagcataga caaaatatcg ccgataagcg tatggagctt
      421 caaaaggcac aagaagcaag gcaggcacag cgaaaggctg acggatcagc gacaacggga
      481 gctacccgtc cccatgagcg accagccacg gcttcaacca ttccaaagcc gagtatggaa
      541 aaaacgcagg aagtcaaacg ccctgccaca gcgaccactt caaaaacaag tgagccagtc
      601 aagacaagtg ttatcaaaga gcgtccgcta tcttctggtg cttctgatag aaaagcgacc
      661 cagtctgtac agccagtaca taggcagaat gtagaaaaag tggtatcgca ggaaacacgc
      721 cagaactaca aagcagagcg tactacaaag acacagacct ttgaacaatc aaagcgtaca
      781 acagaacata ccaaaaagaa ccgtaacctt gtaacgaaga aaggacagaa gaaaaaatga
      841 aactgaaaca tatcgctatt atcggcagtc tgtttcctat cctcttttct ctggtacttt
      901 tctttggtgt cctcattagt gcggacagcg acgacgagaa cagcaatttt tcttctggca
      961 ttacgggtat gaacttatcc gcagaagtct tgaaacatca gcctatggta gaaaaatacg
     1021 ccagagaaaa cggtatttcc gagtatgtca atgtgctact tgctatcatt caagtagaaa
     1081 gtggcggtac ggcagaagat gttatgcaga gttcggaaag tctgggacta ccgcccaact
     1141 ctttagatac gaaaagctca atcaagcagg ggtgtaaata ttttgcgtcc ctgctttctt
     1201 cctgcaaaaa tcaaggtatc gacgacttga atgtagcgat acagtcctat aactatggcg
     1261 gtggctatgt gggatatgtg gcaggaaaag gaaagaaaca cacctttaac cttgcagaga
     1321 gtttcgcccg tgagaagtcg ggtggaaaga aagtaaccta tacgaacccg atagccgttg
     1381 cgaagaacgg gggctggcgg tatggctatg gaaatatgtt ctacgtcgaa ttagtcaatc
     1441 aatatttgac tgtggcacat tttgataatg cgacggcaca agcgattatg aatgaagcgt
     1501 tgaaatatca aggctggaaa tatgtgtatg gtggcagtaa cccgaacact tcctttgatt
     1561 gtagcggact tgtgcaatgg tgttatggaa aagctggtat ctccttaccg agaaccgcac
     1621 aagcacaata tgacgctacc caacatcttc cactctcgca ggcaaaggct ggggacttgg
     1681 tatttttcca ttccacttat aacgctggaa gttatgtaac acacgtcggt attcttgttt
     1741 cgccgacaca gatgtatcat gcaggcgacc cgataggata tgcagaccta agtagtagtt
     1801 actggcaaca gcacttaatc ggtgcaggac gagtaaaaca atagaaagga cttgaaaata
     1861 tgtttaagaa gaataagaaa caaacagaaa ctatcaaaga accaaaagaa aaaaaggtgc
     1921 gtactgttaa ggtaggcaca cataagaaaa ccgtgattgc gttgtgggtg gtgcttatcg
     1981 caagcgtgag ctttggagtg tataagaatt ttactgctat cgaccagcac accacccatg
     2041 aaaaagaaat cattgagctt cgcttgcagg acaccaacgg gattgaaaat ttcgtgaaga
     2101 actttgcgaa gtcctactac acatggaata acagcaaaga agcgattgaa gcaaggacgc
     2161 aggcaatcag cggttatctg acaaaggaat tgcaggactt gaatgttgat acaatcaaaa
     2221 cggatatacc aaccagctcc acggttacag atgtgattgt atggcatatc gagcagtcgg
     2281 gaacggacac tttttctgct acctacgaag tagatcagca gataaaagag ggagaacaga
     2341 caagcaatgt gaaagcaacc tataccgtga aagtccatgt ggacgctgac ggggacatgg
     2401 taatcgttca gaaccctacc cttgccccag caatcgaaaa atcggactat gaaccaaaga
     2461 caccagaagc cgacaacagc gtggacgctg acacagtgaa tgacgctacg gcatttctgg
     2521 aaactttctt taagctgtac ccgacagcca cagaaaagga acttgcctac tatgtagcag
     2581 gaaatgtgct tgaacctatc ggcagggact gcctttattc tgaactggta aaccctatct
     2641 tcacaaagga cggggacaat gtgaaagtca agtttgccgt gaaatccctt gacaatcaga
     2701 caaaggcaac acaggtatcg caatatgagc ttgtgttaca taaggacggg aattggaaaa
     2761 ttataggata atcaagagtg ttcatttatt gacttatttt cttttgtgcg gtatattttt
     2821 atcgtaatgt cggaaatata ccgcattttg aggtggacaa tgattaagaa cagaattaaa
     2881 gaatttagag caaggtatga tatgaaacaa gaggacttgg caaaagctgt cggtgttcga
     2941 cgggaaacga ttggtaatct tgaaaaagga cgatacaatc cctctctcgt attggcttgg
     3001 aatattgcaa agacgtttca tgtaacgatt gaagaagttt tcacagtaat agacgattga
     3061 atttaggagt gataccatgt taggactaaa aaaacgagat attaagaaag ccttgaaagc
     3121 aggtgcaaaa gctggtggtg tttcattggc tgatgtacga gctgatattg aagcgacgat
     3181 tgatgaagct atgaatagta ccgacccaga agtgcaggca aatttcaaaa agtattttgg
     3241 aaataaacgc cctacaccag aagaatatat ttataaaatt acaaaaaaga caaaagttta
     3301 atgttgaaaa accttaacga aaattaagct atgataaaat ctatgcagag aggagttata
     3361 acatgggaag tgttttgatt atagacgatg ataaagaatt atgtacgtta atgaaaaaat
     3421 gtatagagca agaagaaatg tcagctttgg tggcaaatgg tggtacagag ggtttacgtt
     3481 tgatagatga aaataaaaat ttattttctc ttgttatttt agatgtaatg atgcctgata
     3541 tagacgggtt tcaagtttta gaaaaaatta gggaaacaag taatattccc gttctaatgc
     3601 tcacagcaaa aagtggtgag gacgataagg tttttggact gcgtctagga gcagacgatt
     3661 atttgacaaa gccttttagt attaaggaac ttatggctcg tgtcaattca cttatccgac
     3721 gttacacgac tttaaatcct acatttgcag aagaagattg tataacttta aaaggcatga
     3781 aaattgataa agtgaaccgt actgttttag ttaataatat ttctgttgaa cttacaagta
     3841 aggaatttga tttacttaca tttcttgctt ctaacaaggg acgaatattt acaaaaaagc
     3901 agatttatac acaagtatgg aaagacgact atgcttttga tgataacaat ataatgtctt
     3961 tcatcagcaa attaaggaaa aagattgaag cagacccaga acaccccttt tacattttga
     4021 cgatacgagg ggttggctac cgctttaaca aggaggctta attatgaata cttatctgct
     4081 tattgccttt gttttagcac ttgtaataat cgcttgtctt gtttataaac tttatcatat
     4141 taacgagcaa atttctttta tcaaagaagt gcttgttgac ataaaatctg gtaaccttaa
     4201 cagacgtatt ctagtacaag aaaacgatat aactaaacag atttgttatg atataaatca
     4261 aatagcaatc aataatcaat cacagctcat tcatcaaaag caatcggaac aagcgtacaa
     4321 acgactaatg acaagccttt cacatgatgt gaaaactcca cttgcttctt tagtcggtta
     4381 tttagaagcg gttgaaagtc aaattgtaat cggcaaagaa aaagacgaat atattcatgt
     4441 tgcgtctgaa aaggcacaac atcttaaaca ttttgtagaa cacttatttg aatgggtaaa
     4501 attagactct ggggagcaga tttttcattt tgaaattttg gatttgaacg aattgactcg
     4561 taatattatg actgattgga ttttaatatt ggaaaatagt tattttgaat atgaggttga
     4621 gataccagaa gaagaatatc aagtgcgaat tgatataaac gcatacactc gtattatcaa
     4681 caatctttta caaaatataa ttgttcatag tgagggaaac aaaatgatgt tgcgagttct
     4741 gaaagatcag caacgaatta aagttattat aacagataat ggaaaaggaa ttgcttctga
     4801 taaattgcca catatatttg aaaggttgta tcaatgcgat aattctcgct ctaccaaagg
     4861 aaatggtcta ggattggcaa ttacaaagga acttgtcaac gcacataaag ggacaattac
     4921 tgcaaatagt attctaaatc atggaacaga atttatccta tcatttccaa aggctctgta
     4981 aaaatgcaga gctttctttt tgtgaaagca agataaaaac aaggttacgg caaggttttg
     5041 gcaaggttca tatactataa tttaggtgta agtggtaatt ctaataaatt gccacttaga
     5101 tagaacagta aatagaaagt gaggaaattt gtatgagtga tactgtgata aagacaaagc
     5161 aattaaccaa aaagtacgga aaacaaaccg tagtaaataa ggttaatctt aatgtaaaga
     5221 aaggtaggat ttatggcttg ctgggacgta acggagctgg gaaaacaacc ataatgaaaa
     5281 tgatattagg attgacaata actacttctg gaaatgtaga agtttttgga aaaaatatag
     5341 acggaaatga gaaaaggata tattccagaa ttggtgcaat tatagaaaca ccggggtttt
     5401 atcccaattt gactggaaca gaaaatttag aaattttcgc taagttacga ggaacagctt
     5461 cgcctaatgc tgttaaacac gcattagaag ttgtgggtct gccctataaa gacaaaaaga
     5521 cgtttgcgaa atactctctt ggtatgaaac aacgtttagg tattgcaaat gcgatattac
     5581 atgaccctga attgttaatt ttggacgagc caacaaatgg gcttgacccg attgggattg
     5641 cagaagtacg agatttcata aagaaactca gtgtagaaca cggaaaaaca attttaattt
     5701 cttctcatat tctttctgaa atagcagtgc ttgcagatga tataggaatt attgataaag
     5761 gtgttctttt agaagaaagt agtatgagtg agttagaaaa gcgaaatagg aaatatattt
     5821 cgttgcaagt ttcggacatt ccaaaaacaa tactgatatt agaacgtcaa tttcatgtga
     5881 aagattattc agttcaagat gaacagacgg tgcaaattta tgacacgaca ttgaatatgg
     5941 gagaagttaa taaagctctt gtcatggaag atataacagt tatcagttct ggactttgta
     6001 acgatacctt agaagattat ttcaaaaaga ttacaggggg tgagggtatt gcttaagctg
     6061 gttaaacttg aattttgtaa gttgcgtcgt aaaaaactta tttggttgat gttactatct
     6121 gcttttgtca tgccattctt tgcattttta tattttggtt atttgggaca gacaaatatt
     6181 gatcctagac tattttataa gtggtctgca tttggatgta cattattcat tatactgcca
     6241 tttatattag gcttatttag cacaatgctt atgcatgatg aaaataggta tgatatgcta
     6301 aaacaacttt ggattgtgcc agtaagtaaa atgggatatt ttttcagtaa atttttcgtt
     6361 gtattgattt attccgtttg ttttatgctt attacagcat ttgcttctgt actgtttagt
     6421 gtcattccga actatgtagt atttgaatgg agcagtgttt tgtttttgtt ggaaaggtgt
     6481 ctggaaattg ctgttttgac atcatttgca atactaccca ttttagcaat atcttcttca
     6541 caaaaaggat atattttccc tctttgcatt acattgattt atgtatttgc tggattttta
     6601 atcacaccga taaattcttg tctacaccca ctgtcttgta tcagcgttat tattgcaaga
     6661 aatgaagcaa ttttaggatt gtcacttcca caaaataatg tgccattagc acttttaagc
     6721 atttgtgtat ggggaattgc ttctacttta cttgctaaaa tcaaattaag cacgagaaag
     6781 taaggtggaa aaatgagaat acttttatgg gcagaatttc aaaaactccg ccgttccaat
     6841 attattatgt ttactatctt ttcaacaatc ttaatcgctg taattgtttt gataagcggg
     6901 gtgacaacag tatctgaaga acagttaaca acagctatgg ctggctggta tatgacaatt
     6961 acacaagtct gggcgactat gttcgttctt ccagccgtaa ttgctctttt ggggagttat
     7021 atgatttgtc gtgaggaaca agacgataca ttaaaatctt tacgtttgat tccagtaaat
     7081 gaaacaaaat tgacaactgc aaaaatgatt gttgcattgg tatttagtat gctggtgtat
     7141 ttgttacttt ttatgattac atttgtaaca gaagctattt tgcatttctc tgatttatct
     7201 atggcaatgt ttagtgaatt tttaaaaatg tatttattag aggggattgg cgtgtttttt
     7261 gccgtttccc cgattattgc aatcgtacca tatctaaaga aaagttattg gttggctcta
     7321 cttttagcag aaatctattc atttgctgga ttatttatga gtatgtcgaa taccctgaaa
     7381 actttttatc ctatcacagc aatatttggt atttcggggt attacgaaac aactattcag
     7441 gattattttt gtagtttggg aatattgatt ttttgcggtt gtatctcagc tatcttatta
     7501 aaaaatttaa accatagaca aaggaaaaag gctaatgaat aaactatgta gaataagaaa
     7561 agggaaaatc ttttttgggg tttgtggtgg tttagcaaag tgttttaatg tagacgttaa
     7621 aatagtaagg ttactattta ctataacggg ttttattttt cccaaaagca ttattgcata
     7681 tatcattctt atgattgtta taccgaaaga accaaaaaca cgttaaatta taggttattg
     7741 taacttacgg attgtcggct cttacttgct acctgcggag cagatcagca aacaaaaaac
     7801 tagtagaacg ctactaaaaa gaaaaatagg gaaaacaaga agctgattac ttcttgcttt
     7861 ccctgctatt caagataccg tcaatcgtac cttgaatgat ttttaattcg ttatcactca
     7921 ataagtcaag tgatgtttct atctgacggc gagctgtact tttctcaact ttctctgatg
     7981 gatagaaata ttcatcaacc gatatattga acatggtaac taaatcatgg aataattgaa
     8041 aacttgggtg tgaaccgata ttttcaatat ctgcgatatg tcgttctcca tagaatacct
     8101 tatcgcctaa atcgttacgg gatagccctg ctttttcacg ggcttctctg attgccagtc
     8161 ctaatggtct gaaatcaaag gtttgtgttt ctttattttt tctcatttta aaccacctag
     8221 catgagttta aacttcatgc catgtattta gaatgtgtta aaagtgcata tagtatgcag
     8281 taataattcg aaaggtatta tcgtgtgcgg taaataaaaa aaaccgttct tagataatat
     8341 gcttttgcac gtcttaaaaa cgataacggg acggttttgg atataaccaa agctgtcctt
     8401 ttcttttggt atagctctgg tttgaggggg ctatgccatg attacatata gaagaacgct
     8461 acgtcgttct caactggata attatttctg tctagtgctt caatcatcat acccattgca
     8521 tgagttaaaa attcatatag atagcattta tataacagtc tgtttgtgta gttacattca
     8581 aatgggctgt ttttatttta tagaaataat tttttgaaaa tcttggtagt ttgccacctc
     8641 tttgggaagt tagggtgcgg aaagggggaa atgaacaaaa gttcatttca atccagcaca
     8701 gaaaggaggt gagcaaatga aaacatcttc tttcaaacaa gccattgaag cacagtttga
     8761 ttgtctttca aaaaaggtta tcaaacgagc tgtcaaaaaa ggctatcgtg atatgaagcg
     8821 acgtgaaaaa cgagaatgtc cattttctga tattccagat tatgagcagg aacgctttgg
     8881 aacatttgat aaatacgaaa gtgattacac cgtatttaat attttaggca ttgaagtatg
     8941 ggttgaggac gaccagttaa gcgaagctct taaaacattg acagaaaaga aacgcaacat
     9001 tattttgttg tcctacttta tggatatgtc tgatagtgaa atcagcgaat ttatcaaaat
     9061 cccacgttct aacgtccagt atcatagaac aaaaacactc gaaacaatga aaaaaatcat
     9121 ggaggaacgt aaatgaagaa acatgagcat ttgccttttg aggtaatctt atcagccaca
     9181 gagggcgaac cagaagcagt tgaaaccgtt ttgaagtttt atgacggtta catttccaaa
     9241 ttatgcttgc gtaaattgta tgacgaatac ggtaatgttt gcatggttgt ggacgctgat
     9301 ttaaaaaaca gagttcagac agcattactt gatatgctga tgaattttga aattgtagtg
     9361 atgtagtaca cataagtgaa gtttggtact tccccctttc ctgccacttc acttgtcatt
     9421 ctattaaaat caatttgact agaattgatt ttgataggct gaatagccga tagttctttg
     9481 aaaattgaat aaagcagaca gatacgtttg tgaggtagca agccacgggg actgtacgcc
     9541 acgacctttc caaaagaaag cgagcgaccc acgcagtgat ccgagagcga ccgttggaaa
     9601 agtcgctttg ccacgaccta ccctcacaga ataatgatac gtccgcatgg tgcggttctg
     9661 cccacaagaa tgggaatagt tgagatacta acggagcttt ccaaagccgt ctgtctgctt
     9721 gtaaaaaatg acacacagta aagggggtgc atagattatg aacaatactg atgtgcctat
     9781 ctgggaaaaa tatacgctga cgattgaaga agcgtccaaa tacttccgta ttggcgaaaa
     9841 gaaattgcgt aaattagctg aagaaaatct tgacgctggc tgggttatcg tgaacggtaa
     9901 tcgtattcag ataaagcgaa aacaatttga aaaaatcata gatacattgg acgaaatcta
     9961 atgtcattga gccgtgaata tggtataata aggtatagcg tatcacggct cattccatga
    10021 cggaaaggag ctttacacta tgtctaatgt aaaacgaaaa gacagtaaaa atcgcaattt
    10081 gcgtaatgga gagagccagc gaaaagacgg aagatacgtt tataaatata ccgatatata
    10141 cggaaagcca caatttatct attcttggaa acttgtaccg acagataaga cacctgctgg
    10201 aaagcgtgat gatatatcgt tgagggaaaa agaagcacag ataaaaaaag accttaacga
    10261 tggtatcgac acagtcggcg gtaaaatgac agtctgccag ctctacgaga agaagaacag
    10321 ccaaagaaag aacatcaaga gggctactga aaaaggacga ttgtatctta tgaacgctct
    10381 gaaaaatgac ccgttgggta tgagggcgat tgataccgtc aaacagtcgg acgctaaaga
    10441 atgggctatc agaatgagtg aaaaaggata tgcctataaa acgattgata actataagcg
    10501 ttccttgaaa gcgtcatttt acatggcgat acaagacgac tgtattagaa agaacccgtt
    10561 tgagtttaag ctaagtgatg ttctggaaga tgataccgaa cagaaagtta tccttacacc
    10621 agagcaggaa gaacgcctgc ttgcttttat ggaaacggac aagatttaca gtaagtatta
    10681 cgatgaggtt gtgcttctgc tggaaacggg acttcgcatt tctgaatttt gcggattgac
    10741 gacacatatt gatatgcaga acagaatact caatatagac caccagttat tgaaagatag
    10801 cgaaatcggt tactatattg aaacgccaaa gaccaaaaac ggaaaacggc aacttccatt
    10861 aacagaacgg gcttatcaag caatccaaag aatattaaag aacagagaaa aggcacaacc
    10921 gctgattgta ggtggttaca gcaatttcct attcttaaac cgtgagggct tgcctaaagt
    10981 tgcaggaaac tatgagggca tggtgcgagg actaattaag aagtataaca agtatcacac
    11041 ggataagtta ccgaacatca caccacattc attccgacat acttattgta cgaatatggc
    11101 aaacaaagga atgaacccaa acactctgca atatcttatg ggacacgcta acataactat
    11161 gacacttggc tattacgcac acggtacatt tcaatctgca aaagcagaac tggaaagact
    11221 ggcttgttaa tatcggagct tatatttact actcatttac tacttttggt tgcattttca
    11281 tgctggtaaa tgccagctta tgcaaggtat cttccaaaaa gaaaataccg ataaagccct
    11341 aaaatacggg atatatcggc atttaccaat ttatgaaaag ataatcagaa atttagtaag
    11401 ttttttgact aaaaacagat atagatatgt attgacaatg ataattatta ttgataaact
    11461 ataaaagtga aaatgataat aattttcatt ttaattaata aaagattttt aaggagggag
    11521 aatgaagaaa aggtatttag tcataggaat aataattttg tcttattttt ctttatttat
    11581 aggagctgaa gatataaata taatgcatat atttgcaaaa aatcagcata aacttatgat
    11641 atttatcatg agtagaatac caagattaat aagtatattg attgctggtg taggaatgag
    11701 catagcaggt cttataatgc aacagataag taagaataaa tttgtatcac ctacaacagg
    11761 tgctacaata gatgcagcac aatttgggat agtaatatgt atgcttttag taccaactgc
    11821 ctctattttt acaaagacaa taatagcatt tgtattttct cttgtaggga cttttacgtt
    11881 tatgaaaata atagggaagt tacaatttaa aaatataatc tttgtaccat tagtaggtat
    11941 aatgtttgga aatataatag gttctatgac ggattttatc gcgtataaat acgatttgag
    12001 tcaaaatgtt agttcttgga tgcaaggtga cttttctatg atacttaaag gaaattatga
    12061 gatattatat atcacaatac cccttataat attagcatat atttatgcaa ataaatttac
    12121 tgttgttggt atgggtatgg attttgcgac aaatttaggt cttagttaca aaagaattgt
    12181 caatataggt ctgataatag tagcacttgt tacagtatgt gtagtcgtta cagctggtaa
    12241 tatccctttt ataggtctaa tagttccaaa tatcgtatcc ttgtacatgg gtgataatat
    12301 aagagcaagc atatggtata ctggtttact tggagcgata tttgtactta tatgtgatat
    12361 ttttggaaga attattattt atccatatga aatatctata gggcttacag ttggagttat
    12421 aggaagtata ctattccttt atttaatatt aaggaggaac gtaaatgaag cttagtctca
    12481 atcaaaaaat ttactttgta agtatattac tattcatatc aatagcactt tttgtaattt
    12541 atggaataga catgaatcac ttagaatatg cattatcaca aagaatacca aaaatatttg
    12601 ctatggtatt aggtggagga tgtatagcat ttactactgt agtgtttcaa acaattacaa
    12661 acaatcaaat attaacacca agtgtactag gtttagattc attgtatgta atgattcaaa
    12721 ctataatagt attcatattt ggttcaagca gtagtctgat tataaatgaa aattataatt
    12781 ttattattaa tgtagctatg atgattggag cttcattgtt attatataag acgttatttg
    12841 aaaaaaataa aaataatatt ttctttttaa tcctagtagg aatgatgttt ggaaccttat
    12901 ttaaaagtgc aacaacattt attcaggtaa tgatagaccc gaatgaattt ttagctttac
    12961 aaactagtat aatggcaagt ttaaacaata taaacactaa tgtattgtta atagcattta
    13021 taataattat tgccataata ccttttatat atgatgaaat aaagtattta gatgtattgt
    13081 cgttaggtaa agaacaagca attaatttag gtgtagattt tgataaagca gttaaaaaaa
    13141 tgataatatt aatagcaatt ttagtctcaa tatcaacagc acttattgga cctatgacat
    13201 ttttaggact tttacttgct aatataacaa gagaaatttt taaaacatat aagcatacat
    13261 acttgatttc gggttctatg ttgataggga tgattacatt gataataggt caatttttta
    13321 tacaacatgt atttaagttt gatacaactt taagtgtagt aataaatttt ataggaggaa
    13381 tttactttat tcaattacta ttaaaggggg caaatagatg atagagataa aaaatatatt
    13441 taaaagatat aagaataaaa atgttgtaga tgatgtctct tttagtatag aaaagggaaa
    13501 gataacatct tttataggac caaatggagc tggaaaaagt acagtactat caatagttac
    13561 aagacttata ggaggtgatg gtggagaagt aataatagaa ggaaaaagct tgactaatta
    13621 tagcaataaa gaattagcaa aaaaaatagc tattcttaaa caatctaaca atataacttt
    13681 aaagcttaca ataagagaat tagttggatt tggaagattt ccatatagtg aaggtaatct
    13741 aactaaagag gatgaaaatt atattgatga agcaatagaa tatatgaagt tgactgatat
    13801 acaacataag tatttagatg aattgagtgg aggtcaaaga caaagagctt atatagcaat
    13861 ggtaatagct caagatacag agtacatatt acttgatgaa ccactgaaca atttggatat
    13921 gaaccattcc atacagatga tgaaggtact tagaagtctt tgtgatgagt tagataagac
    13981 tatagtatta gtaatgcatg atataaattt tgcatcatgt tattcagata atatagttgc
    14041 attaaaaaat ggaaaagtta aaaaagtagg tcgtacagat gagatagtaa atgaaaaagt
    14101 tttagaagat atttatgaga tgaattttaa cattaagaat ataaatggaa atcgaatctg
    14161 tatttatttt taaaatattg tatatgaaaa taaacgtgct atatgatttt ttataaagtt
    14221 tttaaattaa aataaagatt aataggagag aggaaaatat tgatatgaat aaaaaagcag
    14281 caatagtagc agcagtagct attataggat tagtgacagt gtttgcatta ggtggaagta
    14341 aaaagaatga atcaaaaaca tcagaaaatt caaataatac tatgaagata actcataact
    14401 tgggagaaac agatgtaaaa ttaaatccta aaaaagtagt ggtatttgat tattcggctt
    14461 tagatacaat ggatgcatta ggtgtagctg agaatttagt aggacttcca aaagcgagtt
    14521 taccagcatc cttagaaaaa tataaggatg acaagtatgc agatttagga ggattaaaag
    14581 aaccagattt agagggtata aagtcggcaa atccagattt aattataata aatggaagac
    14641 aagaagattt ttatgagcaa ttgtcaaaaa tagcaccaac aataagtaca agtaaagatg
    14701 ataagaagta cttagaatct gttaaaaata atatagacaa aatagcaaaa atatttggag
    14761 tagaagaaaa agcaaatcaa gaatttagta aaattgaaaa gaaaatagaa actttgaata
    14821 aaaaagtaac agataaaaat ttaaatgctt taactataat ggtaaatgaa ggtaatttaa
    14881 gtgtatttgg tgaagaatca agatttagta tattatacaa tagttttgga tttgaaaata
    14941 aagataaaaa tataaaagaa tcaagtcatg gacaaaatat tacttttgaa tatatagcta
    15001 aacaaaatcc agaagttatg ttcgttatag atagaggaat agctactggc agtgatgtaa
    15061 aagaaagctc aactgcaaaa tctgttttga acaatgatat aataaaatct atggatgcat
    15121 ataagaatga taatataata tatttagatt caccaacatg gtatgtaaat gatggtggtc
    15181 taacatcttt aaataaaatg atagatgatg cttcaaaagc ggtaaattaa taatccgata
    15241 taaccaaaat gtatttttta taaaatgatt atcaaataaa ctaggagttt ttaagaactc
    15301 ctagtttatt ttttgcataa aaaagatgta gtttaaacaa taatattatc tataataaga
    15361 taaatctatt caaaatctaa taaaatatga agttatacta gacaaaaaga at
//
LOCUS       DACXMZ010000079        15364 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.079, whole genome shotgun sequence.
ACCESSION   DACXMZ010000079 DACXMZ010000000
VERSION     DACXMZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.079"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            134..322
                     /locus_tag="JD016_09730"
     CDS             134..322
                     /locus_tag="JD016_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999914.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCEKGQKKFSEETSHHTCFKNII
                     EAIHISKSLIMDEISKRD"
     gene            582..1781
                     /locus_tag="JD016_09735"
     CDS             582..1781
                     /locus_tag="JD016_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HAU4999915.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            1833..2336
                     /locus_tag="JD016_09740"
     CDS             1833..2336
                     /locus_tag="JD016_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU4999916.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            2443..2760
                     /locus_tag="JD016_09745"
     CDS             2443..2760
                     /locus_tag="JD016_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999917.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            2918..3877
                     /locus_tag="JD016_09750"
     CDS             2918..3877
                     /locus_tag="JD016_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAU4999918.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPSDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            3992..4777
                     /locus_tag="JD016_09755"
     CDS             3992..4777
                     /locus_tag="JD016_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999919.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKYKKQQEQLEHFKKIINEKR"
     gene            5063..6388
                     /locus_tag="JD016_09760"
     CDS             5063..6388
                     /locus_tag="JD016_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAU4999920.1"
                     /translation="MPMSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYE
                     GNEYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIA
                     PGDNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNM
                     RRKMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGII
                     VPPVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGG
                     LPLGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYI
                     KAGFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIF
                     LGQSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(6463..6891)
                     /locus_tag="JD016_09765"
     CDS             complement(6463..6891)
                     /locus_tag="JD016_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999921.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(6982..8358)
                     /gene="hydG"
                     /locus_tag="JD016_09770"
     CDS             complement(6982..8358)
                     /gene="hydG"
                     /locus_tag="JD016_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HAU4999922.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(8359..9444)
                     /gene="hydE"
                     /locus_tag="JD016_09775"
     CDS             complement(8359..9444)
                     /gene="hydE"
                     /locus_tag="JD016_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HAU4999923.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(9480..10688)
                     /gene="hydF"
                     /locus_tag="JD016_09780"
     CDS             complement(9480..10688)
                     /gene="hydF"
                     /locus_tag="JD016_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HAU4999924.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDSGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(10703..10954)
                     /locus_tag="JD016_09785"
     CDS             complement(10703..10954)
                     /locus_tag="JD016_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HAU4999925.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(11216..12202)
                     /locus_tag="JD016_09790"
     CDS             complement(11216..12202)
                     /locus_tag="JD016_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HAU4999926.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(12232..12927)
                     /gene="pxpB"
                     /locus_tag="JD016_09795"
     CDS             complement(12232..12927)
                     /gene="pxpB"
                     /locus_tag="JD016_09795"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAU4999927.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENRDEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(12947..14167)
                     /locus_tag="JD016_09800"
     CDS             complement(12947..14167)
                     /locus_tag="JD016_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAU4999928.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIISFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(14196..14987)
                     /locus_tag="JD016_09805"
     CDS             complement(14196..14987)
                     /locus_tag="JD016_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HAU4999929.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
BASE COUNT         5439 a         2151 c         2190 g         5584 t
ORIGIN      
        1 atttaatgtc actgatagtt agaatattta agtattaaaa gatgtgtata aataataatg
       61 atgtcagttt ataatgatat taaattgagg tatactgttt ctctaaatta aaaaataaag
      121 gagtaaatgt gttatgaatt atcatacaaa agaagaattg gtggaagcac tgagagtagt
      181 atcttcaagt attattaatt gtgaaaaagg acagaagaaa ttttctgagg agacatctca
      241 tcatacatgt tttaagaata ttatagaagc aatacatatt tcaaaatcat taataatgga
      301 tgaaattagt aaaagagatt gaagcttatt ttgcaattta acatgtttta atatacattt
      361 tattatataa gaaaataagt aaatatacat gttataaaat attggaataa tatacaatta
      421 taaatttgtt aacattaata tctatatgtt aaaatatagt agaagatgtt agtaaataat
      481 aggatgagtt ctattttaat acaattaaat taagcttagt ttgaaatttg tatctaaaaa
      541 gtaaagtaat ctagaattaa gaaaatggag atagaattat aatggaaaag ttatattata
      601 caaatcagta tataagagat tttacagcag aaataattga aataaaagaa gtagaaaata
      661 agtatcatgt tttattagat aagacagctt tttttcctgg tggaggaggt cagtttggag
      721 acctgggaaa aatagagaat gatgatgtaa taaatgtgta tgaagatgga aaaaaaatat
      781 accatgtatt agataaaaaa cctataaaga tacataaggt aaagtgtagt attgattggg
      841 atagaagaga agatggaatg catcaacatt ttggacaaca tgtattgtct ggatgttttt
      901 ttaaaacttt taatgcaaat acgacaggtt ttcatttggg aaatgagtat agtacagtag
      961 atatagaagg taatttagaa gaggataaaa ttagagagat agagttgttt gccaatgaaa
     1021 ttattcgtca aaatatacaa gtagagacat tacttccaac gaaaaaagaa ctaaaaaaga
     1081 tttggttaag acgtgatttg ccaaatactg atgaagagat aagagtaata aaaataggtg
     1141 atttagatac gaatgcatgt tgtggagtgc atccaaaatc aactggtgaa ttgagaatga
     1201 taaaaataaa gaaatgggaa aaacataagt caagtacaag aatagagttt ctagcaggta
     1261 aaagggcagt ggatgaagta ttgaagaggg atatatattt gactaaaata tgtagatatt
     1321 taagctgtag tgaggaagat gctataaatg gtattgtaaa tctaaatagt aaagtggaag
     1381 aggctttaat taaaaaaaga aggcttgaag aagtagtatc tacatatcag gtcaaagaaa
     1441 tgattgaaaa agcaaataag gttggcaata tttcggtcat taaaaaaatt tatgatgatg
     1501 aagatttgaa atatgtaaat aagatagcaa ataaaattgc agaatatgaa aatagtattg
     1561 cattattagc tgttaaattt aatgataaaa taaatttagt ttttgcatgt tctaaaaatt
     1621 taaaagatat taatatggga gttttattaa aggatgcaat aagtcttata gatggcaagg
     1681 gtggtggaag taaagtatta gctcaaggag gaggtaagaa taatggaaat ttagaggcta
     1741 cacttgatta tgcatttatg aagatagaaa aaactctata aatatattat ataaaaaacc
     1801 ttagtagata aaatagatat ggaggaataa gtatggagca acaaaaggac tacatagagt
     1861 tatatgaaaa tgaagaacta atagagataa gagaagcacg tatggatgat ttggatacaa
     1921 tagcaaaatt taattataat ttggctaaag aaactgaagg aaaagaactt gatatggatg
     1981 ttttaactaa aggtgtaaaa gcattgttat tagatgagag aaaaggaaaa tatcatgtat
     2041 atacagtatt tgataaagtt gttgcacaga ttatgtatac atatgagtgg agtgattgga
     2101 gaaatggaaa tttcctttgg attcagagtg tatatgtaga taaggaatat agaagaaaag
     2161 gtatctttaa ttatttattt aattatataa aaaatatatg tgataaagat gaaaatatag
     2221 ttggaatgag attatatgta gaaaaagaaa acataaatgc aaaagcaaca tatgagtctt
     2281 taaatatgta tgagtgtgat tataatatgt atgaatacga agtagtacat tcgtaatata
     2341 aatttgtaat actgttatac tgatgttata aagtattgat tatattgacg attatatgaa
     2401 ataaactgga tattataatt agaattttta gggggtataa aaatgggcgt tttaaaagga
     2461 atgttttatg tatttttatt tttagattta gtgtcaatat tctgtttctt tttcaataaa
     2521 ggaaaaatag catctaataa aattgtgttt aatgcaatag gagtacttac atttgtactt
     2581 tgttttatgc tttttagtta ttatcctaat aataatctta ttggaaaatt tattgcttct
     2641 ctatttttta tatttggagt tgcaggagtt atgttgaaag aaaaaaattt tttatatgct
     2701 agacttcttt taactgtagt tatagttttt tctacattaa gattgttcgt tattcaataa
     2761 aatgtatttg tatgaaaatt tattaaagtc tacacattta agtgtaggct tttttgattt
     2821 tttgtttgaa aattaaggaa tttttaagga aattttaaaa tcgtcttaaa tatttattga
     2881 tatacttaaa atattaatag cgaaaggaga cttatcagtg atagaaagta aaaagtcaga
     2941 aaaattgtta ctgtcaatta tatacataat atttttggta tatattttaa tgattataag
     3001 aataatttta tttagagatg taccaattta cgctattttc aagggaacat ttaggaatgt
     3061 aaatttaatt ccattttata caatatatca atttatagta gatagtaatc ttgactttat
     3121 gaaagctact gtaaatatta tagaaaatat aggtatattt attcctatgg gaattttttt
     3181 acctatagta tgtaaaaatc taaataaaaa aactataatt atagtaatta tacttgttag
     3241 cctagcattt gaattaacac aatatatttt tgctcttgga agtagtgata tagatgatgt
     3301 tattttaaat tcattaggtg gcataattgg aattaatatt tacataaaca tgaataaatt
     3361 atttcccagt gatattaaca gatttaaggc tataataggt actagtttag ttttaggagt
     3421 tatagggtta ggagttattt ctaaaaatta tcataactta ctaactttta aatttagacc
     3481 tgatagaaaa atatcaaaaa tattaattga agaaaatagg gaaataatta aagatataaa
     3541 taaagataca gtagatatag ttgggacatt tgagagcttt aaaaaaggaa taataactat
     3601 taaagcaggt tctaataaca aagtaaagag accagagaat ttaattgatt ctgatggaaa
     3661 tataagaatt tatttaaatg aaaatacaaa attagtatct tatataatta ccaaagaatc
     3721 aaaaatggat atagttaaat atgaggaatt tgatgtgaag aatttagatt tattaaggag
     3781 atatgatact attaatgttt ggattgataa agaaaatcag ccaaaggata aacatggtat
     3841 tatggctagt aaattattaa ttggattgta tgaataaaat attaaacaag aataaaagaa
     3901 taacaaatat aaaaaacgaa aaaactattt ctataattat ttttacgttg tgcataaaat
     3961 aagaacatta gtgaaggagt agagataaat tatggagata ataaaatata tcttgtactt
     4021 ttttatatat tcatttttag gatggacagt agaaagtata ggatgttcta tagcaagcaa
     4081 aagaataatc aacagaggtt ttttaaatgg tccaatatgc cctgtttatg gttttggagc
     4141 ggtaattgtg atatcactat tgggaagatt taataatgta gtaatagtat ttctattagg
     4201 aatgatttta actacgatac tagaatattt tacaggattt attttagaaa ccttatttca
     4261 tgctaaatgg tgggattatt ctgacagaaa attcaatata aagggtagag tatgtttaaa
     4321 aaatgcaata tactttggtg taatgtctgt tttaataata agatttatac atccatttat
     4381 aaaatatttt gtaagtataa ttccatatag aatattaatt tcaatggcta taattacaac
     4441 actttggact atacttgatt tgattgttac tattataaca cttaaaaaat tagatataaa
     4501 gttaaactta ctagacgata taattacaga ccttaatgat ataaatgtga aattggataa
     4561 atttgataga ggagaaatac aagctttatt taaaacaata aataacgacg aattagaagt
     4621 tagagaaaaa ataaacaaaa taaatagtaa gttggaccgt atagagtcta atattatttt
     4681 acaaaaaaga gttataaaag cttttccaca tataaaatat aaaaaacaac aagagcaact
     4741 agaacatttt aaaaaaatta ttaatgagaa aaggtaatta tacaatttaa tgaagacgaa
     4801 tagaataatg gtatttataa gatgattttc agaggactct ataaaaatag aatagagtcc
     4861 tttatttttt gaaaaataat gtttatttga taaaaataaa atatattgtt tctgaataac
     4921 aaatattttg aaaattataa aaacatatat tgacaatttg tgtcaaaaat actaacatta
     4981 agtatataaa gaattttata tgagaatatc tttataaatc gcaataattt taaaacataa
     5041 atttatttaa agaggggatg atttgccaat gagcgagata actagtcaaa tgataagtac
     5101 agaagaaaaa aaatatgttg ctaaaactca aaagatacct tattatccag tagcatttaa
     5161 atcaggtgat ggtgcaatgc tatatgatta tgaaggtaat gaatatgtag attttttagc
     5221 aagtgcaggc tctgcaaatg taggtcatgg taataaggaa atctcacaag cagttaaaga
     5281 acaaatggat gatataacac aatatacact tgcatatttc catagtgatc ctcctgtaaa
     5341 attagctgag aagcttgtgg aaatagcccc tggagataat gataaaaaag tactatacag
     5401 tgcaactggt tctgcttgta tagatgctgc tataaaatta gcaagagggt atactggaag
     5461 aacaaagata atatctatgt gtgaatctta tcatggaagt acttatggag ctatatctat
     5521 atctgcttta agtacaaata tgagaagaaa aatgggtcca ttgttaccag aagtttatca
     5581 cttccattat cctgataaaa atagaacagc taaagaatgt ttagatgaga tagaatatgc
     5641 ttttgcacac tatctaccag cagaagaggt agctgcaata tttatagagc caattgcagg
     5701 agatgcagga ataatagtgc ctccagtaga gtgggtacaa ggtttaagta aaatatgtaa
     5761 agaaaatgga atattacttg taagtgatga aatacaacaa ggtatgggaa gaacaggtaa
     5821 gtggtttggt atagaaaatt ttggtgttga agcagacctt atagttcttg gaaaatctgt
     5881 tggaggtgga ttacctctag gagcagttgt tggaagaaca gaaataatgc aaagtcttga
     5941 tgcaccagca catttattta ctttagctgg aaacacaaca gtatgtgtgg ctgctttaaa
     6001 atctatagag ataattgaaa aagaaaactt acttcaaaag agtatagaaa tgggagatta
     6061 tattaaagca ggatttgaaa aactaaaaga aaaatacgat ataattggag aaataagagg
     6121 cataggatta tctataggag ttgatatagt taaaggaaaa ggttctaatg aaaaacatcc
     6181 agatgctaca gctaagatat gttatagatg tatacaaact ggattaataa tgatattctt
     6241 aggacaatct actttaagag tgcaaccacc tctagtaata actaaggagc aagtagataa
     6301 agcaatgaac atcatagatt ctgcaataga tgattattta aatggtagaa ttggtgatga
     6361 agtatatgaa gtaactcaag gttggtaaaa taaatattaa atgaaaatag gagttatctc
     6421 aattttagaa aattatttct gaaatgagat aactctttta agttacccac aaataagaaa
     6481 attgtttgat tcattaaaga ctatatttcg gatgtcgttt ttgtgagtat cagagacagt
     6541 attacgagaa taattacctt tattcgtact atctgttaat ttagttataa ttaagatagc
     6601 taaaagaata agaattactg tgtataaagc aataacatat ggttttcttt tatatttgta
     6661 tttgttattg aaaaaagaat ggccataatt atttttattt actttagatg tatttgtatt
     6721 ctttttcttg ttacctttct ttttatcatc cttttttcta tttttagggt aaggaatgac
     6781 tttgtgtgta ttatcaagtt tgatattttt atttttttga atattgtttt ccaggctagc
     6841 ctttttttct ccaaattgaa tagttttatt ggtattgttg ttatctttca atgttaatct
     6901 ccctccctta aactttccct ttagataata ttactactaa cctatggaat atacaataaa
     6961 taatatataa gtaataaaat tttataggta taaatctctt tctccacgct ctaaggcagt
     7021 catattgtta gatactatgt ttcttatatc ttctctttct atcttaccta tttcttctgc
     7081 gataagtttt ttacccataa ttttaagttc ttcatctcca taatcgatta aaaattcatt
     7141 taatgtagta agagcatttg gtgtacatac attatggatt tgtcgacttt tagctaagct
     7201 catgaatcta tcgccagttc tacctttacg ataacaagct gtacaataac taggtatata
     7261 tccatcagta atcagttctt taagcacctc aacagggctt ctatggtctc caacttcaaa
     7321 ttgttctgta ttgtcaccat cttcatatgc tttatatcct ccaacacctg ttagagaacc
     7381 tgcactaatt tgtgatacac catattttag taactcattt ctcatttcag cagtttctct
     7441 agtggacatt atgattccag taaaaggaac agctaatcta gttatagcta ctattttttt
     7501 gaacatatcg tcagaaacta agtgaggaaa atcttctaag ctcatacctt cagctttttt
     7561 caatctaggg aaagatatag tatggaaacc tacaccaaat ttttcctcta agtgttcgtt
     7621 gtgcatcata agtcctagaa cttcaaattt agggtcagat aaaccaaata aaactcctgc
     7681 accaacatca tcaacaccag cttccatagc tctatcaaaa gctgttaaat ggtaataata
     7741 gtcatttttt atagattgac catgcatttt tataaatgtt ggtttgtgat aagtttcttg
     7801 gaataatata taagttccta ttcctttttc ttttagtaat ttgtactcat ctacacttgt
     7861 agctgctatg ttaacgttta cccttcttat atttccgttt tcattatatg ttgaataaat
     7921 tgcatctaag caatctaaga tgtaatttat atcacaattt ttagggtctt caccagcttc
     7981 tagagcaagt cttttatgac ccattttttc taatatttta acctcttcct ttatctcttc
     8041 catggtaagt ttttttcttt taaatttgtt gcatttacta aaaccgcaat aaacacattc
     8101 atttacacag tagtttgata cataaagagg ggcaaataaa accactctat taccatatat
     8161 ttcatttttt atttgcccag ctatactaaa taatctatct agttgttttt tatcttctac
     8221 ttcaagaagt gtagctatat ctttataact aagtttttcc cttctatcag ccttatcaag
     8281 taccttttca atatcatcag aagttgaatt ttttgcatct tctaataaag aattaataag
     8341 ttcatggttt atgaacattt acaaacgcct ccattcaata ttatcaccgc gagaatagtc
     8401 aacttcaagt cctaatgact tgatgttttc ttctaaactt tggtgatatt cacaagcttc
     8461 ttgaccagta aaggctttgt tatcgtaaag agaatattgt tttctaaatt cttgaggaga
     8521 aaggtttggc ataattacat tgcaaccagc taaaagacct gcatttctac cactagggtt
     8581 tatagatgcc aaggctgttg tagcgggtaa taaaactttt ggaagtagaa gtcttgtaat
     8641 tgaaagcata agtaatgtct tctctaaatc tccatgttta taatccttaa aaattgtatc
     8701 atggtgtgga atgaaaggtc ctattccaac catatgagga tttaactctt ttaggtataa
     8761 taaatcacga acaaggtctt cgttgttctg aaatggtgat tcaaccataa atccagcacc
     8821 tacctgataa ccaatttctt taaggttttt taggcattct tttctagttc taagctcaat
     8881 gtttggcgga tgtaattttt tataatgact gtctgtagca gtttcatgtc ttaaaagata
     8941 tctatctgca ccacataaaa agtatttttt atacgaatca taactctttt ctcctagtga
     9001 tagagttatg gcacaatcct catatttatt ttttatactt gaaacaattt cacatatttt
     9061 atcatcagta aaatatgcat cttcaccacc ttgcaaaacg aatgttctgt atcctatttt
     9121 ataacctata tcacaacatt caagtatttc atcaagtgaa agcctgtatc ttatgatatt
     9181 tttattagaa gattgtattc cacaatagta gcaattattt ttacaatagt ttgtaagctc
     9241 tataagtcct ctcaagtaga ctctatttcc ataatgttta tttctcatgt cgctagcttg
     9301 tttataaaga tagtttctta aattttgttc gaagttatat tcctcattgt cgatattttc
     9361 tattaaatat agtagttcat caaaacttaa tgaattaatg ctgtaaagtt tatctataaa
     9421 gtatttaatc ttatttttag tcatattcaa atatataatt attaagtata gttaaatttt
     9481 taataattga aaagttcaag tgatctttcc aaaatacctt ttacatatgc tattaagata
     9541 ccatagttta caataggtac attgaaagat tttgcctttt ctattctaga taacattcca
     9601 gctctattca tcatacaagc tccacagtga actacaaggg catatttatt tatatcttca
     9661 gtgaaggata ctccagaact aaattcaaaa gtaatgtttt tgccagtttt ctttctaatc
     9721 atattaggaa tttttactgt tccaatatca tcagtttgtc tatgatgggt acatccttcg
     9781 gccatcaaga ttttatcacc atcttttaaa ttttctaaag cataagcgcc attaatcaat
     9841 tctttaaggt ctcccttttg tctagcaaac aatatagaaa aagatgtaag aggaatatct
     9901 tttggtgtat ctttatctac ttgtggaaat acttgagagt cagtaataac aagttttggt
     9961 tttttgccta aattagatag agtttctttt aaactatctt cttttgtaac tatagaaatt
    10021 gcaccactat ccaaaatatc tcttataact tgctgttgag gaagtataag tctacctttt
    10081 ggagctgctt tatctattgg aacaactaaa acaactaaat catttggttc aattaaatct
    10141 gataccaatt tgaactcagt gctatcctta ggcaaaactt ttattatttc attttttaaa
    10201 ttttcaattc caattttatc tgttgatgag acagaaacaa ctggacattt aactttattt
    10261 ttagttaagt ttaaaatttc tgattgattc ttaatagtat caattttgtt gagaattaga
    10321 atatgaggga tattcttatc attaaatttt tcaattagtg acaaatcttc ttgtgatata
    10381 ccaatttggc aatccactac tagtaaagct atatctgtct tttctaaaac atctagtgat
    10441 ttacctattc taagttcacc aagttcacca acatcatcaa gaccagcagt atctataaga
    10501 acacaaggtc ctataggaag gatttccatt ggtctaaata ctgggtctgt agtagttcca
    10561 gcaatatctg agactattgc agcactttga tttgttatag catttattat gctagattta
    10621 cctgcatttc ttttaccaaa gagacctata tgtactctta ccgattgtgg agtagaattt
    10681 agactcattt gtactcacca ccttaaactt ctttttttga tatactagtt tttacagata
    10741 catgaggtat gttgcctaat ttaccagtca aactattaat agtatccatt tcgccaacaa
    10801 ctgctataca tacgattgag acaccttctt cttcgcaagg aatgcccatt cttcctttaa
    10861 taataccttt aaaacctgat acaacctcat tgaatttaaa ttgacattct tttggctctt
    10921 ccaaaatagc acttataact gcaacctttt tcataaatat cctcctaact cagaaaatat
    10981 gtcttaaaca aatccaaaat caaaaaactt ttctttgtaa agaaaagttt ctacaaaaga
    11041 aaagttatta attacacaag tataatgtgc tatattaaac tatttcctta cagattagat
    11101 aactcttttc tggtagggtt agcttgtaaa aatttaattt aacaacaagt aactgaagaa
    11161 aatttttata caatttatat aaattataat aaaaattgta tttgttaaaa ttaaattaaa
    11221 ctgctatatc atttaatact tttgccttta caactcttat atttttcata ctcttgtaga
    11281 tatcttgaat cttatcttct tgctctctca atagtctatg tgcttcatat atatctacaa
    11341 gttcaaattt tactacatca tttggctttg cttgtgctaa cttatacaaa tctacactta
    11401 taacgttccc tatcttagta tagccaccac tagtctgtct atctgataac attataatcg
    11461 gttttccatg accaggtact tgtatagctc cataagaaat accatcagat ataatatctg
    11521 ctccattaag gtgctttata ctctctccat ctagtctaca acccattcta tctatttcat
    11581 ttgtaacttt gtatttattt gataaaaatt tctctacttc accgttagaa aaagcatcaa
    11641 attgaggacc ttttacaatt cttagtacga tttctgaact ataagttgga atatattttg
    11701 aacttaattt tttatttatt gtaaaatctt tttcctgaat aaaagtattt atataatctc
    11761 cttttttaag tgctctacct tcaaatccac caattttagc tcttgtataa gttgacttac
    11821 ttcccattgc aagtggaaca tctattccac cagctattga cacataggct ctagcacctg
    11881 atttcacacc tctaaatgat aatacatcac ctggatttat ttgatatgac ctccagtttt
    11941 gaatttcttt tccatttatt ttagccccta aatctccccc agttactgct atttgcgtat
    12001 attcatcaaa ggtaattgta ggacctaaca ttaaaacttc taaagaagcc tcatcttcag
    12061 aatttcctac taatatattt cctactctat gtgcatactc atccattgct ccacatccag
    12121 atacaccata ttgaccatag ccaactctac ctctgtcttg tattaatgtt tggaaacctc
    12181 ctaattcaac tttgaatccc atatatacca ctccctgtac tataacgata tttaaatact
    12241 taagttattt ttttcgattt ctataaattc atctaatgtt attggaacaa attttataaa
    12301 atctccagct ctaagcaaaa tctgttcgtc tctattttca tcatataatt ttaaaggtgt
    12361 cctaccaatt atttgccatc caccaggact atcaatagga tatacaccag tctgggaacc
    12421 tgctattcca acactcccac cataaatttt agtccttggc acttctaatc taggtgtagc
    12481 tattctttca tccataccac ctagatatgg aaatccagga gtaaacccta gcatatatat
    12541 caagtactct ctgcttgtgt gtatttttat aacttcatct acagtgagat tattgtagct
    12601 tgcaacgttt ttaatatcta taccaaaaac ctcatcataa catactggaa tctcatgtat
    12661 tttaggtttt ggtaatacta tactttccaa attatcttct atttttttta aattctcaat
    12721 caaactatca aaatctattt ctaatggatt ataacttatc ataagtgacc tatatgttgg
    12781 taccatttct gttacaatac ctttaagatt cgatatttct attgctctca taaatgaaat
    12841 tacttttttg tttatatctt ctgatatctc atttccaaat tcagcaacca ctgccttatc
    12901 ccctgatatc aagtatctag tttccataaa tgtcacctct tctaaattaa gcaaataaag
    12961 cacctaggct gctaagagaa gttattcctg tatatgcaga tattaaaact actattaatc
    13021 caaatattaa aagccatgta ggatgcttgt aatctccaac aatatctttt cttttagatg
    13081 ctataagcat aattcctaat gtgattggaa gtatcaatcc atttaaagct cctgctaaaa
    13141 ttaataaagt agctggtttt cctataaatg ccattataag tgttgatatt gctataaatg
    13201 atattataaa atatttttca tatttatcta taaaaggatt tagtgtcttt aagaaagaaa
    13261 ctgatgtata agcagctcct ataactgatg taattgcagc agaccataat acaagtccaa
    13321 agaatttata tcctattgct cctgcactaa atttaaaagc tgaagcagct ggattttctg
    13381 ggtcaagttg aagacctttt gaaaccactg ccagtattgc taaaaataaa agaactctca
    13441 tcattgtggc aactaatata cccaaaagag aagatttagt tatttctttt atattttctt
    13501 ctcctgttat tccaccgtct attaatctat gtcctccagc aaaagttata taacctccaa
    13561 cacttcctcc aagtagagtt attattggga atatcagtcc ttttggattt tctggtttta
    13621 ccattcttga tactgcctct ccaacaggag gatgatttga tattgctaca tatccaacta
    13681 taattatcat tcctaatgct aaaaatttag ttatcttatc gactagagaa tttgaatttt
    13741 ttgacataaa tacaaatata gctattaatc cacttaatac agttcctaat gtcatattca
    13801 tattaaacat tacattcata ccaagtgcag ctccaccaac atttcctata ttaaaagcta
    13861 atcctccaag agctactaaa aaagctacta aataacctaa tcctggtatt attttatttg
    13921 ctatatcctg acctctaagt cctgatacac caattactct ccatacattt acttgagccc
    13981 ctataaaaag gattgttgta attaaaatta caaatgaaaa attcggtcca aaatcttggg
    14041 taaattgtgc agtctgagtt aaaaaccctg gtccaattgc tgatgttgcc attataaatg
    14101 cagcccctat aagtgctccg acatcttttt tatttttagt tttatctgta atattttttg
    14161 tactcatatt ttatctcccc ctcatgttat cagtcttata ctatgttctc tgacgaacaa
    14221 acttctatgt tttctaatgg acaaacttct ataccttcta attcaaaacg ctttcttatt
    14281 tctttgacaa actctattgc ttttggattg tccccatgta cacatataga atctgctttg
    14341 atatgtataa cttcacctgt taaagtttct acagttcctt cttttaccat cctaacaacc
    14401 ctatctattg catgttttgt atcatgtata actgctcctt ctacatttct tggaaccaaa
    14461 aaaccattat tatcataagc tctatctgca aatacttcat ttgcaaacct aagtccaatt
    14521 tctttagcag aatttatcat ttcactatta tatagcccca aaagtataat atctttatcg
    14581 acttcatata tagcttcaca aatccccata gctaactctt tatctttagc tgccatattg
    14641 tataatgctc catgtggttt tacatgttgt atattacatc catttgaact tgcaaatgcc
    14701 attaaagcac ccaattgata ttttgtataa tctttagctt cctgctttgt tattttcatc
    14761 tcacgtcttc caaatcctat taaatctagg aaacctggat gtgcgcctat tttcactcca
    14821 ttttttttag ctagctgtac tgttttttcc atatgactag ggtcacctgc atgaaaccca
    14881 caagctatat tagctgatga aatatatttt aaaacttctt catctaaacc aatcttatac
    14941 gttccaaaac tttctcctaa atcactattt aaatctactt tatacaattt atgacccctc
    15001 ctcgctaaca ttttgataga ataagtagtt ttaattttta aattttgttt attttatata
    15061 ttgcatgaaa tattttatca cttgtattca attttatttt tgcaactatt ctattgcatt
    15121 taattaatat aattttacca tttaaggaat atttttgcaa ttattttaat tgaacattca
    15181 tttgtatttc tttttcaaaa aatcttttta ccatttttta gttaataatt aattattttc
    15241 acttaaaata agggctttaa ccttcatttt taaagtattt ttcattttta atcactttat
    15301 atgcaagaca gaatcatttt tcttcattca tttgtttctt tcatctatat aattatcatt
    15361 cttt
//
LOCUS       DACXMZ010000080        15279 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.080, whole genome shotgun sequence.
ACCESSION   DACXMZ010000080 DACXMZ010000000
VERSION     DACXMZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.080"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            329..2563
                     /locus_tag="JD016_09810"
     CDS             329..2563
                     /locus_tag="JD016_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAU4999930.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSKKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2594..3439
                     /locus_tag="JD016_09815"
     CDS             2594..3439
                     /locus_tag="JD016_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAU4999931.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3679..5412
                     /locus_tag="JD016_09820"
     CDS             3679..5412
                     /locus_tag="JD016_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAU4999932.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5804..6230
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5869..5897
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6510..6662)
                     /locus_tag="JD016_09825"
     CDS             complement(6510..6662)
                     /locus_tag="JD016_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999933.1"
                     /translation="MDNFLQGVLASLVASLIVYLNSKLFKKVKSHSGRSDFSFELKIK
                     FKKNKH"
     gene            6846..7245
                     /locus_tag="JD016_09830"
                     /pseudo
     CDS             6846..7245
                     /locus_tag="JD016_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            7345..7498
                     /locus_tag="JD016_09835"
                     /pseudo
     CDS             7345..7498
                     /locus_tag="JD016_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            7787..9142
                     /locus_tag="JD016_09840"
     CDS             7787..9142
                     /locus_tag="JD016_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAU4999934.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(9260..9928)
                     /locus_tag="JD016_09845"
     CDS             complement(9260..9928)
                     /locus_tag="JD016_09845"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HAU4999935.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(9995..10423)
                     /locus_tag="JD016_09850"
     CDS             complement(9995..10423)
                     /locus_tag="JD016_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAU4999936.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            10575..11123
                     /locus_tag="JD016_09855"
     CDS             10575..11123
                     /locus_tag="JD016_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999937.1"
                     /translation="MYTNFNIDFNNFNKKENAIKFMLMGVLLIILGLLCLTFKTLGIK
                     LISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVLIIACILLFVFPQKIQ
                     SIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGICLILSPLFLSRFIVSI
                     MSFFIILFGLVLFFSGNTARKY"
     gene            11243..12178
                     /locus_tag="JD016_09860"
     CDS             11243..12178
                     /locus_tag="JD016_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAU4999938.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPNDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(12449..13393)
                     /locus_tag="JD016_09865"
     CDS             complement(12449..13393)
                     /locus_tag="JD016_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAU4999939.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            13711..15141
                     /locus_tag="JD016_09870"
     CDS             13711..15141
                     /locus_tag="JD016_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HAU4999940.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGHLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSNFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
BASE COUNT         5835 a         2027 c         2005 g         5412 t
ORIGIN      
        1 ttaaacttaa aattaattat aaaaaaactc taccttacta actatacagc tcacaatcta
       61 tctatttatg aaaaaatatg atataattgc attcaaaaag gaaatctttc aatatactag
      121 aatttaagca ttaactaatt ttatctaata ttattactaa actaaaaaat atttgtattt
      181 gtagcattgg agcaagtaac ttaatgtata ttcattttaa atacttattt aaaatttagc
      241 atttaaatta tttacaaatt aacatttaaa tacttctagc ataaatacag ttgtcatcaa
      301 aatttaaagt atcttatgga ggaataccat gcaaaatcat aattatataa aaatgttaaa
      361 taaaaaggta gaagtattat ctaaggaatt agatagtaga aattttaaat taaaaacctt
      421 aaatgaaata gatttttttt ctaaaatacc aaacagagaa tttatgtacc aacatattga
      481 tgactgtatt tcgaaaaata tacatatagg aatcatgtta ttaaatatcg ataactttaa
      541 attatttaat gatatatatg gatatctagt cggagaaaat ttattaatta ctctaactca
      601 aaacctattg cactttattc cagaatcaaa gggaatagtc gcttatttaa aaggagaagg
      661 ttttttaatt gtacttccaa atgtatcaaa taaagagtta aataatatgg gtgcaactat
      721 tatagagagc gcccgagaat taaaaataaa acttcctaat gaaaaagaaa tatactccaa
      781 tgttacattt agtatcggca gcactatctg gaatggtgaa ccaaacatga ctactttgat
      841 gcttttcaac caagtatata gtgctttaaa aaaggctaaa agtgaaggca aaaatagata
      901 cattctattt gattttaaag ataatagttt ttacaataaa ttagaaaatg ataaggagtt
      961 cttatcagaa atatcacatg ttctacaaac taaacaaatt gaagtatttt accaaccact
     1021 ttataatata aaaaataata ctatagttgg atgtgaggca cttgcaagat ggagacaccc
     1081 tattaaagga cttcttactc cagataaatt tattccttta tttgaacagt ttggtctgat
     1141 aacggaatta gatttgcatt tatttgaaga gaactgtaaa aatataagaa gatggattga
     1201 taacaatgat gtttttgttc caatttgttg taacttttct cgtcttcatt tttttaaccc
     1261 taattttcca agtatgctaa aaggtattac tgaaaagtac agcgtctcta catcaaattt
     1321 tagcattgaa ataacagaaa atatattaat agaaaataca aatacaatta ttcaacaact
     1381 agctgaactt cgttcttatg gtttttctgt atcaatggac gattttggtt ctggatattc
     1441 atcttttggt atgttgcaaa atctaccttt tgatataatc aaaattgata ggatattttt
     1501 atctcataat ttaaatgatt ttaaaaatac aaccattttg tactctataa taaacattgc
     1561 aagagctctt gggatgctta cagtatgtga aggtgttgaa acagaacaac atgtagaatt
     1621 tattaaaagt ataaattgcg atatcgccca aggttattat tatgcaaaac ccatggagtg
     1681 caaagaattt gaaaaattac tatctaaaga gaataattca aagaaaatgt ttataaaata
     1741 ttctgatata ggaaaatctt taatagagaa gatatttgat ggatttttta ttatgcaaga
     1801 tatgaaaaaa ctaagagcta atgtcactga agacattcaa tggtttgatt tttacaacaa
     1861 aactgaaatt acatcttttc ctaaagtaat agaacatttt gaaaaacata ttaaaggtaa
     1921 aaaattttct attctttaca aaagtataac cccacataat actgaatcag attcattatt
     1981 aattagtgga gaagccgttt tatcagatga agcttctgaa catcaaaaat atagcaattt
     2041 ctactttagt gcaaattgta taatttcaga aaataaatta ctcttagcaa aattacattt
     2101 ggatttaatt aaaactgctg gctatataaa tgaatttgaa tcatattcaa tacctaaaat
     2161 gacaaataca catcttgatg aaagtaatat tttatcacaa ttttattcaa tgttgcctgt
     2221 aggtataatt cgctacgatt tatttggtga tatgattatt acttatatga atgaagaaat
     2281 gtttgatatt ataggttata caaaagaaca gttttttggt gagatgggcg gaaatttaag
     2341 agcaattgtt catccagatg atttagactt tgtttataaa aaaagtttgg acatgattga
     2401 cacaaataaa attgaaccat ttttatatag attcataaaa cgagatggtc aaattgtcac
     2461 agttatgtat acacaatgta acctttctgc aattgatgga aggtcgattg cccagggtat
     2521 gtatgtagat gtaaataaaa taaaaaattt aacaaaaatc tagtacaaag tattttattt
     2581 tggagaaatt aatatgttta aagaaatttt tttaagaact tttccaggat ttctaattat
     2641 aagagattct aactacagaa ttatatttat aaatgataat ttaaaaaatt taattaaaag
     2701 ttatatgcaa gataatccac ttggaatgac taatatagaa atagctaaga aactccctga
     2761 taatattgct aaatttttta cggattctca caatattcaa ttagattggg aaaaaaatta
     2821 tccttatgat aaaatttcaa attggatttt agagtttaaa aaagatacta caagctattg
     2881 gaatgtttta gaatataaag tagatgttga tgaaaaaacc tacataatta ctatggccaa
     2941 tgatataaca aaactctatg aagaaaataa gagaaatctt cactactcaa ttactgaccc
     3001 attaactgga gcatataata gaaaatattt aaatgataga tttgacatat ttataggaga
     3061 ttatattgta ctaattgact tagataattt caaaatgata aatgactatg aaggtcataa
     3121 tgtaggagac aagattttat gtgattttgt ttcactttta aataaagaat taataaattc
     3181 tacttctatt ataagattag gaggagatga atttattgtt atattctcta gtgatgttga
     3241 caaaaattat gtttattcac agctagaagc cttaagagaa aattttttaa aagtattctc
     3301 taagtataag tatctttcat ttagttatgg tgttgataca gtaaaaagaa atttaaaatt
     3361 gacaatagtt gaattggata aaaaaatgta taaaaacaag gagaaaaaca agaaaaaatt
     3421 tgataaaaat gattattaat attgttattt taaaataaat taaaattatc tttctttttt
     3481 taaacttgta cattagttta taaatatgta tctatacaag atattgacat taagtactga
     3541 agcatatatt gtttcagttc ttttttgtta aaattaactt atttgacaaa gatatcatag
     3601 tttaacaaag cttttaattt aaatatattg atgtataata taattaaaat ataatagcaa
     3661 aaagaaagga ggaatatcat gagcatactc ctaaaaaaag cacccaaatt agcaaaacat
     3721 attataacaa gtttttatat aaatcgggat attgatgaag tattaaaata cctttgtgaa
     3781 aatgttacat ggattggacc aggagaacag gaatttctca cttcttttaa tgaaataaaa
     3841 aattattttt atgcaggtca agatgaaatt ccttcttgtg atataaataa tgatatattt
     3901 gaaactgtaa gtgaagatga gaatagatgt atggtattag gaagatatac tgttcgaact
     3961 aaagaaaatg ctcaaatgat tttagaggta aatcaacgtt gtacatttga gataattgag
     4021 gatagaggaa aattattaat aaagcacatg cacatatcaa acccttatgg tgaaatgcaa
     4081 cttgatgaat actttcccac taaaattgga actcaaagtt atgattattt gcaaagatta
     4141 ctaaaagaaa aaacagaagt tattgaaatg attacaaata atatcaatgg tggcttaaaa
     4201 ggtagcaatg atgatagtac atactcattt ttctatgtca atgagggatt gcccaaaata
     4261 ttaggataca catatgatga gttcatggaa atgagtggtg gttcagcagt gggtgctgta
     4321 tatccaccag acttacctaa agcactagaa gattgtgaac gatgttttgc aaaaggacct
     4381 acatattcat ctgaatatag aatccgaaaa aaagatggaa cattaatgtg ggtattagat
     4441 tctggaatga aatcgttaaa tagtgatggc atagtaaaaa ttaatagtat tattactgac
     4501 ataacccaac taaaaaacat agaatctgaa ctaaaattag aaagagagcg atatcgtatt
     4561 gctttgcaaa atattacaga tatcatgttt gagtatgata ttgaaaacga tacccttata
     4621 aaatatcaac gtgtagaaat tgacaaaaaa attgaattag aaaattttga aacaaaaaat
     4681 tactccaaac tattagaatc tggtaaaatt attcaccttg atgatattgg aaaactacta
     4741 gaagtacttc atggaaatct tcgtgaaaca attgagataa gacaagtaaa ttcattaact
     4801 aaaaatgaat ggagatggat tcgtgttcaa tgttcagtta tatatgatag tgaccataac
     4861 cctatcaaaa caataggcgt tttaaaagac ataactgaag ataaatctaa attagaaagc
     4921 ttaattaatc aggctcaacg tgacccacta acacaattat ataatcaaag agtatcacaa
     4981 aatcttattg aagagtatct atgtagttct gattccaaaa ataatgatgc tctattagtt
     5041 attgatattg atgattttaa aactgtaaat gatacttttg ggcatttaga gggaaatgaa
     5101 gttttagttg ctgtatcaaa aatactatta cataacacac atgataaaga catagttgct
     5161 cgaattggtg gagatgaatt tacaatattt ataaaatcat taacaaagga tttaataata
     5221 aaaatcacta atgatatttt aaatgatgca agtaaaataa aagtaaagga caatcataaa
     5281 ataacattaa gtattggtat tgccttcact gatgattcaa ctaaattata taaagattta
     5341 ttttctaaag ctgataaagc tctttatcta tcaaaagcag atggcaaaaa ttgttatagt
     5401 acatacgaat aataaaacta tttatacgac caagaatgaa cctttaaatt taatttcata
     5461 gtctaacaat caaagaattg aagctaatct tatagctagt caactagttc ctagaaaaaa
     5521 tttagacaat atatttattg atactgctca tttttctaaa gagcaaattt atataaaaaa
     5581 ttaaactttt atgtacatac agatactaat gataaacagt tatcattagt atcttctatc
     5641 ttaaaacaaa atgataattt atctatataa aaaagtgctt tgttgacaga gttcactttt
     5701 ttatataggt acagaattta agtataaata catgttttat tctatacatt ttagttaatg
     5761 taaaatttct tgattgtact ctctaacttt tcaacattat actatttata taccacttgg
     5821 ttaatataaa actattatta tagtgtcgtg tggagggcgg tgtgacttat ttacatacca
     5881 cttagttaat ataaaactct ccgccccatt tttcatcata ttaaacattt cctcatttac
     5941 ataccactta gttaatataa aacgaggtgc tttcaaaatt gacgaagact gtagaaaaat
     6001 atttacatac cacttagtta atataaaact tataagttgt tttatcagca aaaacgttta
     6061 tattactatt tacataccac ttagttaata taaaacgata aaagcatgaa taaaattgaa
     6121 gtagctaaaa ttttatttac ataccactta gttaatataa aacccagctt caatttcttt
     6181 agctcaaata atactagaaa gatttacata ccacttagtt aatataaaac cccaaaataa
     6241 actgggcatt ttcaatgctt acacatgtgc aactctctta aatttgcagt gaaccatgag
     6301 tagtgcaaat gataaagctt atcacatgcc ctcaacacct tatatttcaa gcattaagcc
     6361 ctactttgta acaaatatcg ctcactgcaa aactatttac atttttatta tatcataaat
     6421 atattatttt tgaatatctg taccaatttg tggtataata aaaacaagaa gaactacaat
     6481 ctatttagac gtagagtgaa gttcaaatac taatgtttat tctttttgaa cttgattttt
     6541 agttcaaaac taaagtcgct cctgccagag tggcttttta cttttttaaa taatttacta
     6601 tttaagtaaa ctattaaact ggcaactaaa cttgctagta caccttgtaa aaaattatcc
     6661 atacatactc acctcccttc tatacattgg gaggataatc ttttgtatga actccactct
     6721 ataaattgta gattacatct tcttgctaaa aatgttatag catataattc ttacatattt
     6781 tacctatata ttacttttct aaagattcgt ctatatattt ctgtatacat ctactctatc
     6841 ctcgatttgt tacaacccca atcaaaaaag tagttcaact tctaagtttt atacaaagca
     6901 ggaaactcaa ttattattag aaaaatcaaa aaatactcct ataggtatac caattttttt
     6961 gatgctaact cttggattac gttttggtga agctgtatca cttatatggt cagatgtaga
     7021 ccttgataaa aaatctcaca tgtaaatcaa acattagttt atgtatacaa taaaataatc
     7081 ttcaaaggcc ctaaaacccc tagattcgaa agaagattat ttgttcctga tgaacttata
     7141 agtttgctta aatgggaaaa attaaaacaa aataagttaa aattacaggg tatattgaaa
     7201 aatgaatttg atttgatatg cttaaataaa agattcaacc cgtgagttca accaactttt
     7261 gttgttcatt ttaaaaaact attaagagat aataatccta gagatataaa agtacatgaa
     7321 ttaatacatg caaatgcgac tttgatatta ctttcaggta ctaacataaa agctatttat
     7381 aaaatattag tacatgcaga tataaaaata gctatgaata aatattccat attctcactt
     7441 attagatgaa atgaataaag aaatatctga aaatataagc aaaatactat tcaaataatg
     7501 ttcagtttta tgtcaattaa atgtcactca aagtgctttt tttccatttg tcagttaaat
     7561 gtcacttaaa gtaccttttt cctatttgtc agttaaatgt cagtaatata taaataagat
     7621 atatttataa gacccttaaa atcactatta tttaataaaa tttataatta tatactattt
     7681 gctgttgaca ttggtattat accatagttt atcatttata ttagaaataa aattattaat
     7741 taatagtttc gtaattttat aatagattta cagggagtga taataaatgg atacaaaaat
     7801 atctataaat gaaaacatga gtctaggtaa aagatttttt aaatacctag caccatctgt
     7861 agttgcaatg tgggttttct cactatatac aatggttgat ggaatatttg taagtaaagg
     7921 agttggagaa ttagctttag ctgctgtcaa tatatcaatg ccttttatta attttatatt
     7981 tgcagtttct ctattatttt caactggtgc atctaccata attgcaatct atctggggaa
     8041 aaaagatatt aaatctgcta acgaagtctt ttcatttaac ttagtatcaa taattatatt
     8101 atcaataatt atattagcta taactttttt taatttagat agattagcac tatttttagg
     8161 agctacagaa tctacaattg gtatggttaa agattattta ggaataataa tattttttaa
     8221 cggattcttt atagtgtctt attctttaga agttataata aaaactgatg gctttccaat
     8281 attagctact gttggtgtaa taatatctgc tcttacaaac atcatacttg attacttgtt
     8341 tgtaattgaa tttggatggg gtgtaaaagg tgctggtatt gcaactggtt tgtctcaagt
     8401 cttttctact attttcttct taatacattt tttaagaaaa aactctactt tgaatttttc
     8461 aaaatttaga attgatttta agacacttag aaaaattgta tttataggtt ttcctgattc
     8521 aactactgaa ttaagttgtg gtatagttgt tttacttttt aacttaagtc ttactaaata
     8581 tattggagaa aatgctctta tttattatag tgtcataaat tatataaata cattagtact
     8641 tatgactatg atgggtataa ctcaaggtat gcaaccactt actagctttt attatggtgc
     8701 aggaaatata gataatgtaa aaaaattatt aaaaatgggg ataaaagcca caataatagc
     8761 atcagttgct gtatttgcaa tttgtatggc tttttcagga ccaatagtct ctttatttat
     8821 acatccagaa gaaacaatgc tattcaatga aggagttcgt gtatttaaaa tattctctat
     8881 atctttctta ttggtaggaa ttaatgtaat aatttctgga ttctttgtat cagtagaaaa
     8941 accatcaatt tctactgtta tatctcttgg tagagggctt gtaatagttg ttttaagtct
     9001 aatttcaatg atactaatat ttggtggaca aggaatatgg atgactacta ttgtttctga
     9061 gtttatatgt cttattttat cattagtttt tctaaaaaag aatttttcta cattagactc
     9121 taatctaaat aaggttgctt agatgataag agtgacaata tatgaaaata tctaaataaa
     9181 aaatacctat tatacaatct tgcaaatatt aaacctttgc aaaaattagg attatataat
     9241 aggtatttta tttatgtaat tattttattt ctaaatatcc atagccatct tttattaaag
     9301 ttccttttac ttcatattct acaggttttt taaatattgg accatctacg acaaatgtat
     9361 gatactctcc atgttctcca catgggtctg caccagcatt ttttatcttc tccacaagtt
     9421 ctctagttaa tttttttcct aaaaattcct ctcctaagtg ctttaagttt acttttgtta
     9481 caacactaca aaatccactg tctataaatt catatactaa atcctctcta ctctcttgcc
     9541 ataatggtaa atctactttc ataccaactg cttcacatct atcagtatcc cattttctat
     9601 gactttctat atctatatct ccatatgcac atatcgttgc tcccatgttt ttagctttta
     9661 ttaaagccct ctcaaagctc tcttcatatc cttctacgcc acactcagca actaataaag
     9721 gtaatcctat gttgctactt acttgcttaa gcatattata ttctaaatta tgtgtccatg
     9781 actttccatg ttcttcactt atagttgtca agagtgccac aggcttatat ccttttttca
     9841 acattcgatt taatgctaat atactatcct taccaccact aaatgacatt acaaattttt
     9901 catcagccaa ttttaacacc tctttcatct aactttgttt tattaacata attataccaa
     9961 atttttgagt tgcatagcaa attatattaa attgttaatt cactataaag aatgtcccta
    10021 atattaaaag tataacccct atgtatttgt ataaatgcat tgtttcttgt cccaaaatta
    10081 cgctatcaat aaaaaatcca actaaaactt gggatacaag agttaatata agtgtatttg
    10141 atactcctaa atttggaatt gcctttactg taaaatatat tattagacct ccaaatactc
    10201 ctcctaataa caacttataa ttaaaaccat ttatagatac tagacacttt aagtcacctg
    10261 taactattat acttgccaaa aaaaatattg ctcctacagt taagcttata aaggtggcca
    10321 ctaatgctgt tgtacttttt cctagctctc catttataaa agcttcaaga gctgtagaac
    10381 cacctgctaa taatgcaaat attattgccc ataattttgt catggacaaa cctccttcta
    10441 tataattcta ttaatagaag tttatgatac aacctattaa ttaattacta agtatagttt
    10501 aatttttata cactaggctt tatttggata tgaatttctg ttataattgt tctatcgatt
    10561 ttggaggtga ttttatgtac acaaatttta atatcgattt taataacttt aataaaaaag
    10621 aaaatgctat taaatttatg ttaatgggag tattactcat tatattagga ttattatgcc
    10681 ttacatttaa gacattaggt ataaaattaa tttcttggac ttttggtatt gcacttttat
    10741 tctttgcata tttaaattta aaaaatatta atgaattaaa aagatatgct actaaagaag
    10801 aaattaaacc ctctattaat atccaatggg ttctaataat tgcatgtata ttactttttg
    10861 tatttccgca aaaaatacaa agcatatttt cattattttt aggattttat ttaatcttta
    10921 atcaactagt agcacttgta aatagtaaga ataatcctta ttctaaattt acaacttgga
    10981 atatagttaa gattctattt ggtatatgcc ttatattatc tccacttttt ttatctagat
    11041 ttatagtatc gataatgtct tttttcataa tattatttgg actagttcta tttttctctg
    11101 gaaatacagc gagaaaatat taattatttt ctcgctgtat ttttatatta cttaaaatag
    11161 aatttgttta agttatctat ttgtggtaat atgttttttg tacaagctca gcgtacaatt
    11221 ttattagaaa taggtgattt ttttggataa atttaaatat gcttttgata ataaaagata
    11281 tcatacttgg aattactatc ttagaaatac ttttggagaa aaagttttta aggtatctat
    11341 aaatgcagga tttacttgcc caaacattga tggaagtctt ggttatggcg gatgtaccta
    11401 ttgtagcaaa gaaggctcag gtgattttgc tggaaaccct aaagataatc ttatcagtca
    11461 attttataat ataaaagaaa tgatgttaaa aaaatggcct catgcaaagt atattggcta
    11521 ctttcaagcc tatacaaata cgtatgctcc tcttgaagtt ttaaaagaaa aatatgagac
    11581 tatattagaa cttgaagatg tcataggtct ttcaatatcc acaagacctg actgtttgcc
    11641 aaatgatgtt gtagaatact tgtctgaact aaataaaaga accaatttat gggttgaact
    11701 tggacttcaa acaattcatg attctacatc taaaattata aatagaggtc atgattataa
    11761 aacatttgtt gaaggtgttg aaaaattaaa gtctaaaaat ataaaggttg tcgtacacat
    11821 tataaatggt cttcctggtg aagattataa tatgatgatg gaaacagcaa aggcagttgg
    11881 taaaatgggt gtagacggta taaaaatcca tctattgcat gttataaaag atactcctat
    11941 ggaaaagatg ttacaaaatg gtatgttaac tcttatggaa caagatgaat atatcaatct
    12001 tgtctgtgac caattagaaa tacttcctga aactatgata atccatagac tcactggtga
    12061 tggtaagagg gatgaactag taggtcctat atggagccta aaaaaatggg aaattttaaa
    12121 tcaaattgat gatactttaa aggctagaaa ttcttatcag ggctgtaagt ttgtttaata
    12181 caaaataata actatagaaa tggttatata aatatagtcc tttctatagt tatttagtat
    12241 ttacaaaaca aaatattaat ttgtacattt atacaatata taataaaaaa tagatagtct
    12301 aaactgtatc tacttatatt ttatttgtac tgtccacttt attctataaa atttataaat
    12361 agttattttt taatatcaaa tataatttgg tataattttt gttggactct aaaatgtata
    12421 ccaataaatc ctttttaagt atataattct aaatactttc agttttttca atctgcttta
    12481 acttgttgga cttataaaat attcttattt catcataaga aagattaagt gctactactg
    12541 cagatattaa catcaacatc actccaaata acttccctaa ggaaaaactt tctcctaata
    12601 tagtgcaacc aattattact gaaccaacta attctactga tgccacaaca cctgcaactg
    12661 acaactctac acctttagat atacctgctg cataaaaaat atatgcaagt gcagctggaa
    12721 caatgcctaa cattagcata caagataaaa catctaaatc cttagcataa tttaagattt
    12781 cccaaggtct tgaagaagga atcataaata tagctccaaa taagaaactg tatactaata
    12841 ttgttgaact tgaaaattcc tttaaagcat ttttactaat agttggcata agtgcatatg
    12901 ctattgctga taatacccca agtaaaagcc caaaagcgtt taaaccttga aaatccaaac
    12961 gtccaccagt cactgccata attgcaccaa taaaacaaag aataagtgaa aataatttgc
    13021 ttctagtaat attttcttta taacataatt ttgaaaatat tgctaaaaac agtggtgatg
    13081 tatatagtaa aactgctgca attgatacac ccgcaatgtc aattgcttta aaataacata
    13141 aattaaacat tgcctgacat ataattccta ttattactga atatataatt ccttttttgt
    13201 ttatctttaa gatttgtggt gtccttatag atgaatatac gcctaaaact ataaatccaa
    13261 aaaacaatct tgtaaatgct acttgttctg atgtcagtcc atttcccatt aaaaactttc
    13321 caaataatcc aagtgtagcc cataataacc ctgctattgc tataaataca taccctttac
    13381 ttttttctcc cattttaaat tcccttctat ttttatttag tcaatttaca ctcttctttt
    13441 taagcaaaaa ctatgccatc ttaaatcaat atatatcgac tctaaaaccc tagtctttcc
    13501 aataatacta ataaattttc aattttttta tatttatttt tcgcttactt taagttgatt
    13561 tagacttatt ttaattgtaa aatgagttat atataactta tttaaagtta aaatagactg
    13621 aaacttatat ataaatatca aattttaagt atattaatca attaaaccta ttttaatata
    13681 tcattaaaaa tttaggagga aaatataaaa atgaatttag atttggaatt ttatcaaaaa
    13741 atattagaag cttcccatga tgaaatatgt gtttctgatg ataaaggaat aataatatat
    13801 tgtaataaag catttgaaga aaattatggt ctaaaaaaag aagatatttt gggaaaaaat
    13861 gtttcttttt tagaagactc tggatattca acaaaaagcc ccataccaat tgtattaaaa
    13921 acaaagacta aattttcact ggaacaagat actcaaacag ggaaaaagct tatcataact
    13981 gctaccccta tatttgatga aaatggacac ttagaattta ctgttgaaaa ttgtagagat
    14041 ataactgaac ttaataatat aaaaaataaa cttgaagaca caaaaaagca agtaaaaaaa
    14101 tataaatctg aagtagaaac actatacaga actgcactta gaattgaaga tactgtaatt
    14161 atggacggaa tagttatgag accaatcata aacacagtaa atcatgtttc taaaacggat
    14221 gtaagtgtac ttttattggg tgaatctgga acaggaaaaa gctcacttgc tagatatata
    14281 caccacaaca gcaatagatc taatggccca tttataacaa taaattgtgc aactatatct
    14341 ccacaacttc ttgaatctga actttttgga tatacttcag gtgcttttac aggtgcaagt
    14401 actaaaggta aagttgggct cgtagaatta gctaatggag gaactctttt tttagatgaa
    14461 ataggagata taccacaaaa tctacaggca aaatttttgc aattaataca ggatagaact
    14521 tttactccag ttgggagctt aaaaaataaa aaggtcgata taagaattat atctgctaca
    14581 aatgttgatt tagtttcaaa agtaaaagaa aaaaagttta gagaagattt atactataga
    14641 ttaaatgtta tagaaataaa attgcctcct cttagagaaa gaagggataa tctagtggaa
    14701 attataaaat attattttaa taggtactca agtaacttca atctaaataa aactatttct
    14761 aaagaagcta tggatgctat tgcaaattat agattcccag gcaatataag agaattacaa
    14821 aatataattc aaaaaatctt gcttacttgt acagataatc atataacaat agatgactta
    14881 ccaaacattc ttacaaaaaa tatacatatt accaataatg gaaataaaac acatatatcc
    14941 caaattaata aggttattat atctgactca aaatctacta attataaaaa taaaaatttt
    15001 gacactttaa ttaaagagta tgaaaaaaat ataattttgg atgcttatga aaaatttggt
    15061 agttcttata aagtagccaa acaccttgaa atctcacagt ctaaagccaa taggttaatt
    15121 agaaagtata ctaatactta attttaaatt tgataaataa aaacttaaca ctatttacat
    15181 attgtaaatt tggccattta ctgttattat gaatatggtt gaaattattt aaataaatac
    15241 tgttgactta gtttcatata tttaccatta aacttttat
//
LOCUS       DACXMZ010000081        15009 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.081, whole genome shotgun sequence.
ACCESSION   DACXMZ010000081 DACXMZ010000000
VERSION     DACXMZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.081"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            185..580
                     /locus_tag="JD016_09875"
     CDS             185..580
                     /locus_tag="JD016_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999941.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNNTCTTE"
     gene            623..2722
                     /locus_tag="JD016_09880"
     CDS             623..2722
                     /locus_tag="JD016_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAU4999942.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(2947..8157)
                     /locus_tag="JD016_09885"
     CDS             complement(2947..8157)
                     /locus_tag="JD016_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999943.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLNEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYKWTNRMVNFSDFKLDSDGMKFVLGKQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNRKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(8172..10667)
                     /locus_tag="JD016_09890"
     CDS             complement(8172..10667)
                     /locus_tag="JD016_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAU4999944.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIES
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGKNGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(11240..11527)
                     /locus_tag="JD016_09895"
     CDS             complement(11240..11527)
                     /locus_tag="JD016_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999945.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(11547..11822)
                     /locus_tag="JD016_09900"
     CDS             complement(11547..11822)
                     /locus_tag="JD016_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999946.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(12459..12638)
                     /locus_tag="JD016_09905"
     CDS             complement(12459..12638)
                     /locus_tag="JD016_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999947.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(12923..14389)
                     /locus_tag="JD016_09910"
     CDS             complement(12923..14389)
                     /locus_tag="JD016_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU4999948.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNCCIDFFRNKCIEQVRKTYLYGLDGNLIEFKKLLTYM
                     YKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIGDCL
                     PSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEEGAK
                     RLSLLLNAMEFVTITLEDVVFLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDFVLL
                     CKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYLKKG
                     DVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLDIL"
BASE COUNT         4920 a         2642 c         1685 g         5762 t
ORIGIN      
        1 ctagataaga attatttcaa ctatataaac tacacatgta aaattcatgt aaatttacta
       61 atataaatca aataaaatta ctaaactctc taaactaatt ttgtaaaaca tactctattt
      121 ttaacatttg ttaccttata cataagtaca aacaaaatct aactaaatga aaggataatg
      181 taaaatgaat attttaaaaa tgaaaaaaac agggatattt ttaggaggag tactttttgg
      241 tactgtaggt gttaagatac tttcaagtga ttgtgttaaa aaaacttgca ctcgtgcaac
      301 agcaggagca ttgaaagtta aagattctgt tcttgaaaca actacgaaaa ttcaagaaaa
      361 tgtagaagat attttagctg aagcaaaaga aatcaacgag caagaagact tatctacatc
      421 taaggtaaac gttgtacaag atactcttaa atgcgaacaa gaagttctta aaactgaaac
      481 tataaaaact gtagaagata ctaattctaa tagcattaaa tgtgaagaaa tcaaagataa
      541 cgtaattgaa gaaaaaaata atacttgtac aacagaataa atttaatgat tattatgatt
      601 aataatatca ggaggaagta aattgaattt ttatataaaa cacgaaataa gaggtcgtat
      661 acgctttgac ttacaacttg gcaaattaaa caacaagcag tccgatactc tcttatatta
      721 tttaacttct ataaatggta ttaccaaagc aaaagtttac gatagaactg gtgatgcagt
      781 catttattac actggtgaaa gagaatacat aataagagaa atcactaaat tcagttttaa
      841 caatagcaaa atagaaaacc tagttcctga acataccagc agggaattaa acaattatta
      901 tagagacaaa ttagcaaata aactgctagt caaaagtatc tccaaaatat tttttccaaa
      961 gtccttaaga ttttttttaa ttggacttca ttctataaaa tattttaaaa aaggtctctc
     1021 ttccctgcta aataaaaaaa tagaagtttc tgttttagat gccactgcta taggtatttc
     1081 tatttttaga aaagatatca atacggctgg ttcagttatg tttcttcttg gtatcggtga
     1141 actattagaa gaatggacta gaaaaaaatc tatagacgac ttggctcaaa gtatgtcact
     1201 aaacattgaa aaagtatggt taagaaaagg tgacactgaa atcttaattc caatttcaga
     1261 gataaaggaa ggagatttag taagtgtaac tatgggaaat atgattccat tggatggagt
     1321 tatagtatca ggagaaacaa tggtcaatca ggcatcactt acaggtgaat cccttgcagt
     1381 taacaaaaaa gaaggctctt acatatatgc tggaactgta atagagcaag gcaatattgt
     1441 tatgtgcgtc aaggaaaaag ctggtactac tagattccag aaaatagtaa ctatgattga
     1501 ggaatctgaa aaactaaaat cttctgtaga gagtaaattt gaacatctag cagatacatt
     1561 agttccatac agctttttag gaagtatatt aacctatgca attactagaa accctataaa
     1621 atcactctct atattaatgg tagatttctc ttgtgcatta aaactctcta tccctatttc
     1681 agtactatca gccatgagag aatgtaacaa taacaacatt actgtaaaag gtggaaaatt
     1741 tcttgaaggt gttgcatgtg cagatactat tgtatttgat aagacaggca cattaacaaa
     1801 agctcaacca actgtttcag atataattac attccaaaat tataataagg aggatatgtt
     1861 aagacttgca gcttgcttag aagaacactt cccacactcc atagcaaatg cagtagttta
     1921 tgaagctgaa aaacaagggc ttagtcacaa agaaatgcat acagaagtag aatatattat
     1981 tgctcatggc atttcaacaa aagttgatga taaaaaggtt gtaataggta gcaaacattt
     2041 tgtatttgaa gatgagaagt gtactatccc agacggtgag gaggataagt acaataatct
     2101 atcagatgag tattcacacc tatttatggc aatatcaggt aaattatcag cagttatttg
     2161 cataaatgac cctttacgaa aagaagcaaa atatgtaatt tcaaatttaa gagaatgcgg
     2221 aataaaaaag attgtgatga tgactggaga tagcgaaaaa acagctaaat ctatagcaag
     2281 taaagttggt gttgatgaat attattcaga agtcttacca gaagacaaag ccaattttgt
     2341 taaaagtgaa aaactcaaag gtcgcaaagt aattatgatt ggtgatggaa tcaatgattc
     2401 tcctgctatt tcagaatcag atgtcggaat tgcaatgagt gaaggtgctg aaatagcaag
     2461 agagatttca gatattacca tatcagcaga taatttgaat aatttaatcg ttttaaagca
     2521 gattagtaat aagcttatga aaagagttga tttaagttct aagtttatta tcggctttaa
     2581 tttaggtttg attttacttg gtgtaggtgg atttgttaga ccaagtacat ctgccttttt
     2641 acacaacgca tctactgtcg gaataagttt aaacagtatg acaaatttat tggaaaacac
     2701 caatacttat aactaccatt aatctccatt acataaaaca tatgatataa aaaatttata
     2761 attcatcttt taaaaagaat atgtcttaaa attagctttt ttctaatcaa aattatataa
     2821 agcatacttt acaaatttta aactcaactt atttctattt tttacaaaaa aggagatgat
     2881 tcaaattttc attttgaaac atcccctttt gtaaatttct ttaaaattta tatattaatt
     2941 atattttcaa ccaataattg tattttcaac caatctttct tcatagcatt ttaaatcatt
     3001 ttgctttaga taattaaatt cagactctac ataaatacca cagcctacca attcaattgt
     3061 caaattaggt atattttcct ttagtatact agtctgctca taaataattc tttccaataa
     3121 atcaacttgt aatatagaaa aatgcttcat aatctcccca tcactatcta tatctttctt
     3181 gatttcatac caactctttg acaaaattcc cttcaaatca atatacccta gactcctcaa
     3241 tatatctata ctatcgctcc atgcatactt actaggttta tcaaagtatg attctagttt
     3301 cttcttctgc tcatttgtaa gtgaatcata agaaagcttc ctattaaaat gtgccttcaa
     3361 agaaatctta gccatagaat atggacttct accagagttt aatcctgcat ttttgaactt
     3421 ttcaattcct ttatcttcta ctgaagacca tattgagttt attgaatctg aatcattgta
     3481 tttaacatca agaccatgtg taatatttat atagttttgt ggaaatacat attgataata
     3541 gacatctttt ttatcatcat atagagagaa gtatccacta tcatataaat gaatatgcac
     3601 ctttactttt aaactatcct ctaaaatatt tcgtctaagg ttcggtatat ttgcatacat
     3661 ggttacatat tttatggcat caaagttttt atttaatgga acttcaaaac taatttcatc
     3721 tcctgtcaaa agctttttca cttcttcttc attacaatca tatagagaat atgtattttc
     3781 aaatcccaca cgtgatttcc catctaaact gcctgttaat gctaccttca aaatatggtt
     3841 atctgttttt tgccattggt aagcatcttg gtcaagaaac tctctaatct gtctattttg
     3901 ctctttcgcc tgatttataa cctcgttttc atcttctgta atccctacat ggcatctatt
     3961 taagatattt actatattgc ttccaaatgg aaatgtccat agttgaaaag ctgctgcctc
     4021 aacctgacac aaatatctaa cctgatttaa ttcaatttga tggataagtt ttttctgata
     4081 ttcattcatc tgattaccac tatctgacat ttgataaatc aattctccca ttgcaagatt
     4141 atcttttttc ttatcaatct tatcagtaat gataatttga gtttccatca tatcctgtac
     4201 ttttttaaat attatagaaa tctcctctga attttccatt tttaagcttc caaacttcgt
     4261 gcttaattca gttaaaactt ccttcatctt taatttactt atctcatttt caaaaatatc
     4321 ttgattttta actgtactat cgaatttttc tacaaatggt ataacagatt ctttcaaagt
     4381 tttatcttta aattccacca tttgagtatt caattcatct aatttcttaa tattattttc
     4441 tttatcttta attgcctctt taactacctc tattttactc tcaaaactag attttacatt
     4501 aagacaatta ttaacaccac taatcgcagt atttataaga gaacaagcag aacttccaat
     4561 actcaatgca tttttaattt tagatgtctt ttgctgtctt tcaatagctt gtttactttt
     4621 ctctagtgca gctaacttat ctttttgctg tttattattg taatttgcct ccaacatacc
     4681 tatttctttt ttctggattt ttgtgtaaat ttccttatac tcgctttcac ttaacccctc
     4741 ttttgctctt tgaatatcgt cagctttgtc tgcatagtta tcatcatcta tagacttaac
     4801 tataatctcc ttaatttggt caaatttagt tcttctttct tcatcaacac ctgccaaatt
     4861 ataagtcatt ttatctaaag cattttttct atcttcactt agctgttttc caagtacaaa
     4921 tttcatacca tcagaatcca atttaaaatc tgaaaaatta accatcctgt tagtccattt
     4981 gtattcctct tctttagctg aaagattttc cttcaaagat tttgcactgt caaatgcccc
     5041 tagacaagct tgaccaacac cactaaatgc agtcagtaaa ctcaatgcgc tttgtattcc
     5101 agaaagaatt aactgtgttt tcatttgttc ctcaagtttt gcctgtttct tttttagttc
     5161 ctctatgtct tcttgttcat cttctttcat ctttataatt tcttgaatca acaggttcat
     5221 ttttgcatta acagaaagca ttttattctc aagttcattt tttaaaacct ctatttgttg
     5281 atttccagat tcaatttgag ttaggatact ttccttatat gtattgaaat atccttcttt
     5341 taactcatct ttcttccctt gttccaattc tcttaaggtc ttatccagat aatctgtcaa
     5401 agaacgaata tctgtaacac gatttcctcc aatttcactt tcttcaatca tctgcacata
     5461 aaatgctgtc ttctgctcaa gtgcaaataa aatcatactc cattcgctaa aattctgatt
     5521 ttttcctaca aaagattcta ttctatcaga ctgcatcaat ttatttataa ggtattcata
     5581 gatttccttt gcttctttat aaaattcttt ctttgtattc atatcatcca gagaaagtac
     5641 tgctttattt ttcttctcaa aactatagta cttctgaagt atttctctat ttaattgcat
     5701 ctctattttc tcaactgcat gaaaaatttc ataaatatgt gaaagttcta aaatatcagc
     5761 ttctaaaatt cctactgcac acaacagtct aagatttcta taacctttaa ttccagaaaa
     5821 accaaatctc tcgaaaaatt catagaagaa tgtatctatt gacaaagaat actcctgctt
     5881 taacaattcc ttttctttta gtgcatctcc agagtgaccg gcttttccct ctgctccggc
     5941 tgaacctgaa tcttcacgtt tgctaattgt ccagtcctta tgttcttcat aaagagtatt
     6001 taatttctct ccatcatctg ctgcttgctt ctgatttaaa gcatgattaa acacatatct
     6061 atcaaaataa tagtagtttc tatcactatt atccattgga ggagaggtac ctatatatct
     6121 tattgtttct tcatatatac ctctaattcc accaagacct cctgcaccac cagctcctcc
     6181 agagcctcct tttcctgcta aaccatgatt tccatctttt ttacttatag cactttttaa
     6241 taaaggagct tttccaagga gataaacctg tccagacttg cttccaagac ctgcatttcc
     6301 accattacct cctgtgcctc cagttccacc ttgttttcct ttttctgctt ttaccacata
     6361 gtattttgta cctttaacat tagcaaattc ttttgactct tttttagaac ctattttagc
     6421 atttgcccca gcttctccag ttatccctgt atctcctttt tttccatctt gtccatttcc
     6481 accacgtcca ccatctacgt aaagtttaag ttctcctctt ttttctattt ctttaaaaga
     6541 taatatgata tcacctgaag tacctgcatt attgccattt cttccattca ttccgtcgta
     6601 aatagaactt tctccaacca atttttctgc atccttacca gatacgttga ttgaataagt
     6661 cccttctggt atatgtaatt tttctgtaag tagtacaata ttcttacctg agaagacttc
     6721 tttctccata ttacagtcaa ttttcaactc acaatctcca tacatatata tagatgacaa
     6781 aatattaagt tgataaccag atattatttt agtcaaatcc gataaattca ctttaggtat
     6841 aggaatatat atagaaagta cattttcttt ctcatatgat tttatatatg tacaactttg
     6901 aatcttctct atcctatact ctgcatcttc aagctttttg gcttcttctt ccctatattc
     6961 ttcttctgct atccaatatt tgatatcttt caatgtatct tctaaagtat tttttagcaa
     7021 tgattttaac aactcactta gaccaacaat atgagtctca ctacagtaac tatttaactc
     7081 taaaagaaga cgtgtttctt ctaatatatt cactttctgt acgccatcac catatgaaag
     7141 ttcttgtaca aactccataa gctctttcat attattagtc ctactacata aatctgtata
     7201 catcttatct aattgaatct ttttatcttt tagactattt atagaagata gaatggaaat
     7261 agaatcttcc ataccttttt taacactttc tatataagga actaaaagtt tttctaggtc
     7321 tttataggtt acatcaacag ctttttgtat ttctatttta caagaagcag taagcatcaa
     7381 tcccaaatct gttttttcaa atgtttcagg tagatttttc atttcattca acaacttttc
     7441 tggctgttta tctagtggat tacataattt aatagataca ccatctgata atgctacatt
     7501 taataagtac actaattcct tacaaccgtc gtaatctttc tcttgactat ctaggtcttc
     7561 ttttgctttt tccaatatat ttcttatttt ttcttctgtt acattaccaa ttagtgtttt
     7621 aggtatttta ttgataagaa agaagaaatt ttctttatac tttgcataat caccaagcat
     7681 atctttcaaa tttttgcctg tttctatcaa taatgtatgt ctagagtcca gtagggattg
     7741 atagtctaaa ataactacca tcccttttac ctttgaagat gtggaaatag tttcccttac
     7801 aagcatagca ccagcaatca tatactctgc tccacggttg tcaaaaaaac ctgggcaatc
     7861 acataatgaa aaatctctat ttgcaatatc tacaacttct ggacataaag tcttagaata
     7921 tacaccattt ccaatttcga cagctgaatc ttttgcagtt atatatattt gtccagtctc
     7981 ttcatccgtt ttttcttcca tcacacagcc ttttaaataa tttatacaag tactctttcc
     8041 aacgcctgaa gcacctagga ataacaaaat tgacttttct tttaagtcat attttctaag
     8101 ctcgtctctt ccttcttcca aaaggtttac gatttcacta taaagtttat ttgccataca
     8161 tccatccctt tctatatttc ggaacaatag ccttgatatt tttctttaat aagattctct
     8221 tctacctttg aaacagctgt tgataactta gtatttggag cattaaatcc tccattgtct
     8281 cctctatctc catattcagc gtattctctt gctggtcctg ttttaccttc attccagctt
     8341 ccaccactaa attctatatg atatttaatt atactatcta acttataatc ccaacttgta
     8401 aaatatcctg tatagtatac agtcttatct ataccacttg gagaaaatga aagtgtatta
     8461 tatccgtgat atccaccact tcctccatat ccacctaaac ctggtctacc atttggacct
     8521 gcttctccat ctttcactgt tatatcacaa tgattaatta ttctattttt ttgttcctct
     8581 gaatctgaaa tgaagtaaat attacctttc ttaccagcat ttccacctct tccagcattt
     8641 ccaccagctc cacctttttc accttttttg ccacttgtac ctttttcaaa atacccttta
     8701 aacatctgtc cgtggaaata acttgtatat cctgcatcag ctccattact tccagaagct
     8761 cctgtttcac catttttccc atctattcct ttagaaccat ttcctccatt tgcaatcagt
     8821 gttagacgat aatttctaat ttcttcttta atatataagt aaatattacc tgcatctcta
     8881 ccatcttcac catttttccc atacatagaa ttatctacaa gttctccgct ctttccactt
     8941 acattaattt ttacattact acctacatca agattgttag aagcaagaat aatatttttc
     9001 tctgatgcaa agtccattcc aaggttatca tccatataaa tagtatcttt agcaaagaac
     9061 ataaatgtag aaacattgtc atctactttt ctatttttga aatattccaa tacactgcta
     9121 attactaagt ttggaattaa tgctcttatt tcaatacctt tcttatcatt aatattaatt
     9181 gtttttacta taaatggatt taaaatgtat ttaccaataa attctctaat tatctcatta
     9241 ccttcttctc catatgcact taatcttgtt atactgtctt ctaactcttc attttgtgta
     9301 actgtaacat ttgtcaaaat tgaattaaaa tcacttacac tatatacata atccttttta
     9361 tttgcaactt ccattaattt ttctctattt ggtaatgtaa atacctgttt ttgcacttca
     9421 taagaaacta aaactttttc taaattcttt cttattataa tgaagttttg ttccattatc
     9481 ttcttacttg ccttttctat aataaattcc atatgtacag aaggtaataa aatttcccca
     9541 gttccatgtt ctgtatattt acttaaagca tcaattatat ctaagtttcc ttttatctta
     9601 ttttgtaatt tcatgcaact atcttgtaag aataagccac tttgtttggc taatacgttg
     9661 ataaaactct ctatagtaag acttgttgta ctttccaacc agtttttaaa tacttttaca
     9721 acctcatcat attttgctat ttcttgaatc attgttatac ttccaataat attgataaaa
     9781 tcttctgtat acgccttaac ataatcttct aggctagata cgatattgtt ttgaatttct
     9841 tttatcaacg tatctaaaac taatcttgca tctgcactaa ttggatattg gaatgctgat
     9901 gtcttatctt taacttcatc taattctagt acttgcttta ataagccttc cctctcatct
     9961 gatgcaagtg gcttacaaag atgaatattt tttccattat cttttagcaa ttcttcaaat
    10021 aaccaagctt tttcctctat attactgtct aaattttcgc ataattgttc atattttctt
    10081 tttataacat tatatatttg tttttctgtt cttcctaaga aactttttga aatgaaaaaa
    10141 gttatactac ccttaaaatt ttctttatga agacacatat ttaactgttg gaacaggtct
    10201 agtacttttg taagtctaga atctaatata tctgcttctg gtatcaatac aattactgca
    10261 catatagatg cagaattttt aaatacttca cctaatgaaa ctgctgcact aatatcatgg
    10321 gctactcccc tattgtcgct aaaacctgca gtatcacata attttgcatc cattccttct
    10381 attggttttc tcatatttac aatgttcggg tataaagttt gagaaacagc tcctgaacca
    10441 atctctgcca cagggtctaa gcaatcaata tatgatatac cagtatcttc atcttctact
    10501 tccatcatta cacatcctgc aagatagtta attgcagtac ttttgccaca tcctgtccca
    10561 ccaagaatta gaattaaatt ttgattttca gccttattta ttgctacttt tccttcattt
    10621 aataagagtg ttatttcttc taaaagttga ttgttatttt ctggcattta ttttccccct
    10681 tggttatata ttttatttaa ataaagttta atcccttaag tagattacaa agtttttaga
    10741 ctatatttaa atttgttttt tattttttat ttttaataaa tttgcttttt cactcaataa
    10801 ttattcttct ttgtttatta aaataactgt gttgtataat ataatcttta ccaacacagt
    10861 tattttattt aatgcgctta ttttattgta ttttatgtaa tacagttgtt ttttatatta
    10921 tatgtccatt attttcctat ggttattaca tatagatttt taattatatt tgtaccatta
    10981 atttaaacat tcctatacta ctgcttaagt tttacaccta taccagcttg aatattttat
    11041 ttttatttgc ttaaatagtt caactatact ggtttaaata gattatcttt atttgcttaa
    11101 atagttcaac tatactggtt taaataggtt atctttattt gcttaaatta gttcaactat
    11161 actggtttaa ataggtttcc tttagtttaa atagttcgcc tatacctact tgaatagttc
    11221 acttttattt gcttaaattc tataccaata ctggcttaaa cattatacta ccatgattgt
    11281 attgtctatc ttcaactaca ataaaaacag gttctaaatc tttagtagat gttattttta
    11341 attctaaaaa aacattatca gttggtaata tttttacatc ataattattt aaattattat
    11401 tcgtgatact attaaataca tcaccttgac gttcaaaaaa aacattatca actattacaa
    11461 aataaacatc ttcttttcta ctattttcaa cctctatagt attttgttta gttacattta
    11521 gtctcatctt ccacctccta ttaatattac ttaattatat taatacttgt aattgttgca
    11581 ttatgttcat cacaaggaag aggtttaaaa acaactatct ctgctaaata attctgtgga
    11641 ggatttgtaa ctgaagtagc attgcaaata aatttaaaat ttgttcctgc tacaacttga
    11701 gtagaaacaa taagtggttc ataaccaacc ccttctaaca ttcctacagc ttcattaaat
    11761 acttttaaat cttcttcttc gacagctctg aattttgacc atccaccaac aaaaagactc
    11821 atataaaaac ctcctttata tatatattta tttatatata tagtagtacc aattttctca
    11881 atattcaata ttttctgtgt aactggtcaa tgaaaatgca taactggata aaaatactta
    11941 taaaataact taactttaca tataaatagc ttactttaca tgtaataaga ttattctaca
    12001 atatgaaatg catcttgttt tgaatatatt ctaaatacaa taatggatat tttcaattat
    12061 tataattaga aaatatccat tacttaactt gttttattta aaatcacata gagactttat
    12121 tatttttttt attgcatgaa tagctatttc aggtatcata aggtgatata ccccctcctt
    12181 gaaaccacaa aaaacatgac aaactttacg aatcttggct taaaagacca caggtacatt
    12241 ccttatttgt ggattggagt agccataaca agcaaatttt acaattttta tgatgacaaa
    12301 aatcaataaa atcgcttagt atataacacg aaacactatt tgttactatt aagaatagtt
    12361 agataggaag tatgcattat acaaataaag gaatcaataa agaaagttaa aacatcccta
    12421 caaattaaaa atctgttaca tttttctata tttaaacgtt atttacttac aattggagaa
    12481 ataacaggtt ttccttctcg gtcaagtaaa ggcgtaaatc cggctgcatt gccataatag
    12541 tgaaatacat agttcacacc tgtttcttta tccacccaaa tctccgttgc atttattttt
    12601 ccttgtgaat atactttttc aaatctatct tttcccattt tataatcccc catatttttg
    12661 attggtctat accttcttca cttaagtcga aaaaatacat aacttatttt tttaattcta
    12721 atatgagcac gtaaaaatgt caaacagctt atttaatttc ataacttcat gttactgtta
    12781 gtaaccttgt ttgtttactt acactacatt acagcaccat tttatccttt acattattgt
    12841 attgcacaca gtaaaaatgc ttactttatg ggatttttca actaaaaaaa ggattttttc
    12901 tccacagaac atttactatt ttttataata tatctaattt taatctcata tcaacattta
    12961 atgaagatat aacctttttc aaatattgta aagactcctt aaaaatatta gaaaaagatt
    13021 ctgcaaatct ctcaacatct ccttttttta aatacctaag tatctcaaaa catacatcaa
    13081 aacgtgtttt caaaatttca ttatattctt catgaaaaaa tctctctaaa tagattccaa
    13141 atatcatata ttgttttaaa tgcaaaaatg tttcctgtaa tacataaatt tgtgtataat
    13201 ctactatctt acaaagtaaa acaaaatctg gataaacctt acactcttca caattatttt
    13261 gtaagtacct taaatgagtt tctattttat ctacagctat caaatccatt ttatttccta
    13321 gtaaaaatac tacatcctca agagtaatcg taacaaactc catagcattc aaaagcaaac
    13381 tcaacctttt agctccttct tctgtattta tataatcatc atcatattca gaaaaaagtg
    13441 ttacatatgt tcccttgcca ttaattgttt tagcaattcc caattcattt agctcattat
    13501 aggcacttct aactgttgga agagatacat tatatctatt aattatcgaa gtaattgatg
    13561 gtagacaatc tcctatttta atattgcctt caaaaatttg acaaattaca tctgaaacaa
    13621 ttaagtcata taaataacat ttttccaacg ttacagaatt aaaactactt gtatctatac
    13681 tttgtggagc acgattttct accattttaa gtatggattc tctacaagct ttatacatat
    13741 atgttaatag ttttttaaat tcaattagat tcccgtccaa tccatataag taagtttttc
    13801 ttacctgctc tatgcattta ttacgaaaaa agtctataca acaattatcc aagttatcga
    13861 aattataggc caattgtgaa ataatgacat catgctgaat aatctcacaa aaatggtgaa
    13921 tcaggaaatt atttgtttta cttattagtt tatttatata ttttagcctt tgttctttaa
    13981 agactaaata attagaacta tttttttcca tacaatcgat aatctcgttt aattcttcaa
    14041 agtctttaga tgtaaatctt ttaatattgt acatagcaat agaaggaaat attaaactaa
    14101 aaatcttata tatttctgat atttgtaaaa tcttctcata taataagtca gattctattg
    14161 attgtttact cgtactctgg attaaaactg gtctagaacc agcttttgtc tctataaagc
    14221 ccttttctga gagcattttt attacagttt ttatagtatt ttttgatacc atatattctt
    14281 ctgacatgct ttttatagaa tcaagcctat ctccaatagt aattttacca gtataaattt
    14341 gttgaattat atgctcaaaa ataattaaat attgtggttt tctcttcata tttttcctct
    14401 tcgataaatt agatttattg ttttttgtaa ggtatattta cttttattta atagggcaaa
    14461 atagttgctt attttttcct cataatctat aattaatata aaccatttat atatatggtc
    14521 taagttttat aatactgtaa tgtagtaaaa tagcatgttg tagctatact acggcaaatt
    14581 aacataccat tgtagtatat taattggaat aattttaagt acttcaaaat tccatcaaaa
    14641 catatatgtg tttttgatat tatgtatatt cgttaataga atagctatac ttgttttaca
    14701 taataaatta tatgatactt gctatatgta attactatat ttaatacaca taactattat
    14761 acttgctata tgtaattact atatttaata cacataatta ttatacttac tatatataat
    14821 tactatactt ggacatatag ttatcataca tactatatgt aattactata cttatacaca
    14881 tagttatcat acatgctata tgtaattact atacttagac acataactac tatatttact
    14941 atatgtaatt atactatacg tggacatata gttatcatac atactatatg taattactat
    15001 acttggaca
//
LOCUS       DACXMZ010000082        14882 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.082, whole genome shotgun sequence.
ACCESSION   DACXMZ010000082 DACXMZ010000000
VERSION     DACXMZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.082"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            271..4158
                     /locus_tag="JD016_09915"
     CDS             271..4158
                     /locus_tag="JD016_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAU4999949.1"
                     /translation="MAETILSGYLSNLGKYTEGRPAGEWVSFPTTAEHLKEVFDRIGI
                     DGKNYGELHITEYQSSIAGLAGKLTELESLDELNYLSELLKMQFDDDREKFAAAITYG
                     DHTRDLQDIINLAQNLDCYWIYPSVKTEEDYGHYLIEELDELELPEEAKNYFMYEEYG
                     RDAAINDGGRFTEQGYIYNNRNTFTQWYDGRDVPEEYRVTPQPQEQADLDAAAAIPTT
                     ATEQPPVLPIILSSEKPADKMKEITDRLEQGILGIYESDRYADYLRTMSKFHDYSLNN
                     TILIAMQGGNLVKGYKQWEKEFDRHVKPGEKAIKILAPAPFTVKKQVEKIDPDTQKPV
                     FDKNGKAVTEEKEIQIPAFRVVSVFDVSQTEGKELPDLGIKELTGDVEQYQDFFAALE
                     RTSPFAMGFEALSGGVKGRCNYEEKRISINEGMDELQNIKTAIHEIAHATLHDIDKDA
                     PERPDRRTREVQAESVAYAVCQHYGLDTSDYSFGYIAGWSSGKELAELKGSLETIRST
                     AASLIDTIDGHFAEIQKAQDKEQTTEQAQTPQEATIQPEAEAAAPELPEETAPVQEKE
                     AQTEPEADTGANSEAPQTTQPGQAAPAAPYYTINEAAAKRAKDMNSFSDYKQGSATAE
                     YRHYVDEAVQLAERQKQRVDPMYHEKIDSLLDTYARKLAANMNKGYEIDARVPSILIA
                     GGSNFPTRKKEKQNAARDSNYREWQDIQGLLDKIRSTGMGGISADDPQAVQKLEKKLE
                     NLEKSQETMKAVNAYYRKHKTLDGCPHLSPEQLEKLKADMASSWHLGDKPFATWALSN
                     NSAEIRRVKDRIKSLSQQKEIGFVGWEFDGGKVEANTEANRLQIFFEDKPDEATREAL
                     KSNGFRWSPKAGAWQRQLTSNAYYAADYIKAIAPFTGEKPTEIQRAHIRAQKEAAQKK
                     PEQEAIYKVHANPRSDSRDNLYLLQAYIPQEDGKAKIGDVLYIGTPEKCRELMAQLTA
                     GELTQGEVKELYAKTQTAAPEIPMPDPTISVKDMQEYGYAWDGMLPLQQEAAERLFHE
                     DMSVYLLYEDGTEGLVDTLEDLQAHAQKGGLFGVEKETWEALREYNAMKQELRESEPS
                     REALLLYGNEDTFGIYQLKNGKETRDLRFEPYDRIQAAGNSIDRGNYELIYTAPLTPG
                     MSLEDIYTRFNIDHPKDFKGHSLSVSDVVVLHQNGQDTAHYVDSLGYKDVPEFLQPKN
                     YLKAAEQTTEQNYNMIDGQINNTPTAAELEEKAKAGGQISLAEYAEALKTDKERGKPA
                     KQEKPSIRAQLKAAKEQTPKKQARQKTQDLERS"
     gene            4162..5106
                     /locus_tag="JD016_09920"
     CDS             4162..5106
                     /locus_tag="JD016_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999950.1"
                     /translation="MKLQKFENVDVIASLEAIMKQNTAFYQSDFDIDKQILQKAAVSP
                     VPEDKRLLWFSRPSGTCCFRERDVFLKDTRQHNTWRFYGEQTRDTILAYAVELTGTEQ
                     EKIKGNLYELDYLQHFREVIEKSIPADNYTLIYEHGELTKPAGQYFDGNTDPQLGKFI
                     RFEAQPNDPEALKSILREQQKKRTPHTQGDFQLHIAALHDRRIEAEARRIVENVKGLG
                     EPDSPEKTHCAVELSPYFVPLATDKDMERLLSMLPYKSLSFSTLEGRHGVYALIDKSE
                     RNKEMKKSRPSVRAQLQTEKSPKLPKKTAKSKHHELEV"
     gene            5109..5315
                     /locus_tag="JD016_09925"
     CDS             5109..5315
                     /locus_tag="JD016_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU4999951.1"
                     /translation="MIRLTVEEMNLLSIYHEGSKAQLMENMTTALPFMDEDMRPFAER
                     TLQKISPLTENEYAELSIFAADEV"
     gene            5312..5677
                     /locus_tag="JD016_09930"
     CDS             5312..5677
                     /locus_tag="JD016_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU4999952.1"
                     /translation="MTGSKRLTPPQSRKVNSLVKKECCNCERGHCILLDDGEECVCPQ
                     LISYSLLCKWFQTAVLPLDKLLYAELFKTEDKKRCTECGTFFVSKSNSVKYCPDCRKR
                     ITRRQAAERMRKRRAAVTQ"
     gene            complement(5822..6115)
                     /locus_tag="JD016_09935"
     CDS             complement(5822..6115)
                     /locus_tag="JD016_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999953.1"
                     /translation="MKQRQKETISTWLYEEYRRLWIEKGQEPHKRNNREIVAAVMEQI
                     EAAGIWLPEQEVTKYFYRRKTHYRNRIEKELQPKPEMQDKDSQTQKEQGAGSQ"
     gene            complement(6162..6431)
                     /locus_tag="JD016_09940"
     CDS             complement(6162..6431)
                     /locus_tag="JD016_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU4999954.1"
                     /translation="MADQTIPDYETIRAAVAGETWAVEKVLMCYKDEIDRQATVKKRQ
                     PDGTFKLEIDEDMRQYITMKLIEALPQFPLEEMEREEKRNRDKKS"
     gene            complement(6492..7841)
                     /locus_tag="JD016_09945"
     CDS             complement(6492..7841)
                     /locus_tag="JD016_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAU4999955.1"
                     /translation="MAVTKIKPVKSTLSKALDYIQNPDKTDGKTLVSSFGCSYETADI
                     EFEYTLSQARQKGNNLAFHLIQSFEPGEVDCEKAHAIGKQLADAATKGQHEYVLTTHI
                     DKGHIHNHIIFCAVNFVDYHKYNSNKRSYYGIRNMSDKLCRENGLSVVVPGKGSKGKS
                     YAEYQAEKVGTSWKGKLKIAVDTLIPQVASFEELLQRLQAAGYEIKPGKYISCRAPGQ
                     ERFTRLKTLGADYTEEAIKERIAGKRTKAAKAPKEQRGVSLLIDIENSIKAQESRGYE
                     QWAKIHNLKLAAKTMNFLTEHQIEQYADLVSRIEEITAENEKTADALKSVEKRLADMA
                     VLMKHVTTYQKTKPVYEAYRRAKDKDAYRAKNESSLILHEAAAKTLKASGVTKLPNLA
                     AMQEEYGKLQAQKEALYTDYGKLKKQVKEYDVIKQNIDSILRLEKEPERSRETERLQ"
     gene            complement(7802..8131)
                     /locus_tag="JD016_09950"
     CDS             complement(7802..8131)
                     /locus_tag="JD016_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAU4999956.1"
                     /translation="MNGRKRKVQIKFYVTEEERVLIEQKMKLVPTQNMAAYLRKIAID
                     GYIIQTDHSDIKAMTAEIQKIGVNINQIAKRVNATGSVYQEDIEEIKGVLAEIWRLQR
                     LSLLKAR"
     gene            complement(8380..8733)
                     /locus_tag="JD016_09955"
     CDS             complement(8380..8733)
                     /locus_tag="JD016_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU4999957.1"
                     /translation="MAKRPVEKYDFKAFGEAIKAARTGRKESRKQVSDEMYISPRYLA
                     NIENKGQHPSLQIFFELMLRYNISVDQFLLDTPTQKDTKRRQLDALLDDMSDNGIRIV
                     TATAKEISEVEKEDK"
     gene            8862..9083
                     /locus_tag="JD016_09960"
     CDS             8862..9083
                     /locus_tag="JD016_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU4999958.1"
                     /translation="MIRFSERTGVVMEYMSCPEAAKKWGISERRVQILCREKRIQGVS
                     KLGYMWLIPKDAEKPIDGRTKQGKELYHE"
     gene            9076..9768
                     /locus_tag="JD016_09965"
     CDS             9076..9768
                     /locus_tag="JD016_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4999959.1"
                     /translation="MNKVLIIDDDKELCALIKRSVLSEDIEADFCNMGKEGLQKLKER
                     EYQLVILDVMMPGMDGFETLEEIRKESSLPILMFTSKNDSASKVRGLRAGADDYLTKP
                     FDMDELIARIVSLIRRYTRFNQQDGISQQLEFDGLKIDIENRSVTTENGTFELPPKEF
                     DLLLYCAKNQGKILTKQQIYEEVWREEYFYDDSNIMAIISRLRKKLEVNPGSPKYIQT
                     IKGIGYRFNKEV"
     gene            9775..10692
                     /locus_tag="JD016_09970"
     CDS             9775..10692
                     /locus_tag="JD016_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU4999960.1"
                     /translation="MEAVIFLSIIIALFSILVSCFAIRRVKKQIAEITDALIDVKNGN
                     GNRRILSATNELVAPLAYEINEIVVSYESRLSTVRQTEETNRQLMTSLSHDVRTPLTT
                     LIGYLDAAHKGIVTGKDRDDYIETARRKAHDLKEYIDVLFDWFKLNSNEFAMDINIVE
                     AAELTRNILIDWIPIFEDKQIDYNIDIPEQPFRVKLDTDGYMRILNNLIQNVISHSHA
                     DKIEIILSKQEKNMQIRLADNGIGIEKEDLKHIFERLYKCDKGRSEKGSGLGLSIAHQ
                     LVEKMNGTITANSIQGTGTEFTLLFPLAN"
     gene            10829..11755
                     /locus_tag="JD016_09975"
     CDS             10829..11755
                     /locus_tag="JD016_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4999961.1"
                     /translation="MDKNYIIETKNLTKQYGSQKSVADLNIHVQKGRIYGLLGRNGAG
                     KTTTMKMLLGLTKPTSGEVKIWGKSLQGNEKKLLPRIGSLIESPGFYPNLTATENLQI
                     FATLRGIPNRHSIKNTLDLVGLPYKDKKLFSQYSLGMKQRLAIALAVMHDPELLILDE
                     PINGLDPIGIAEVRSFIRELCDTKGKTILISSHILSEISLLADDIGIIDHGALLEEES
                     LAELEQKSSKHIRFTLSDTAQAARILERTFHESHFSIQDDHNLRLHNLDLSVGKIVTA
                     FVENGLEVSEAHTCEESLEDYFKRVTGGEGIA"
     gene            11748..12557
                     /locus_tag="JD016_09980"
     CDS             11748..12557
                     /locus_tag="JD016_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4999962.1"
                     /translation="MLKLIKCEFWKLKRKRLLYALMSLSLFFPLILAYMAKAGTGADT
                     TEHYLQTRFDYVYTMMLGYGLAFLLPCLVGIIAAILFFIERDCDTSKNLRTIPVTNTQ
                     LIMAKISMLFIFSIAFCLISTLSVALFCKLFHVGMVYGMAYKIYMSLIFGVLIVAASL
                     PIVVLIIYFNKSFLLSILLAFFYSIFNWGLMGTIGTSISAAKIAFLNSFPVMCVMNWT
                     SGLMMDNLQKDNLLPEAYAIVPTTFHTIFIMAITVVLSLWLIIRFYKKWTR"
     gene            12563..13336
                     /locus_tag="JD016_09985"
     CDS             12563..13336
                     /locus_tag="JD016_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4999963.1"
                     /translation="MRNIIKTEFLKLKRYSVIKAGIIMTTLSPLLSLFYSTANGGPTW
                     TFDYFMQQVMISNCTLFFPIIIALIAGYIITREYTDDTMKNILTIPIPYKQLLSGKLL
                     ILLLLTISFSLIGCVIALVINIIVGFPGVHFGNLLNMFIRVTGANIGIYISVLPIILI
                     FCCSANNFLGGVALAFVYGYFGTFEGTLLNYYPIKASMILVDPTCGAEYGYTYHIFPA
                     FITIVLTFLISMIILANKKKEPGTLTVGKKKKAVRKKGW"
     gene            13803..14243
                     /locus_tag="JD016_09990"
     CDS             13803..14243
                     /locus_tag="JD016_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAU4999964.1"
                     /translation="MEPNRREFQKQCVFQSFCKRVLHNEACNAHDEIRRRRKREVSFC
                     DLALHEERQLYTTDKYFQAETAEPTYQMAGKEITPKLLLEAIRTLPEEKRTAILLYYF
                     EGMTDVEIGKMFNTSRSTIQYRRTSSFELLKKYLEEHADEWNEW"
     gene            14224..14481
                     /locus_tag="JD016_09995"
     CDS             14224..14481
                     /locus_tag="JD016_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU4999965.1"
                     /translation="MNGTNGNQNDYLENALVPYPVILAASKGDPDAMKMVLQHFSGYI
                     ASLSMRKLYDERGNVYYGVDEDIRERLQARLMRAILTFRAE"
BASE COUNT         4250 a         3251 c         3430 g         3951 t
ORIGIN      
        1 aggaaccttg acgaactaac cgccggggtg cgtcgccact acgaaaacgg atatagcaat
       61 atcggggaat ttatcgaagc ccacgacgac aggctgccgc cggagcttat cgaggaagca
      121 agagccgccg cccacgctgc ggggctgcct ttctctgaaa aagcctaccg ggacggcgaa
      181 gaacccgacc cctatctatt tgacgggagc atgagcatag aggactacga acttatgcac
      241 cgcatgattg aaaacgaaag gagtgaacgc atggcagaaa cgattttaag cgggtatctt
      301 tctaatcttg gaaagtacac cgagggcaga cctgcgggcg aatgggtatc attccccacg
      361 actgccgagc atttgaaaga agtctttgac cgtatcggga tagacggcaa aaactacggg
      421 gaactgcata tcacagaata ccagtcctct attgcaggac tggcagggaa attgaccgag
      481 cttgaaagcc ttgacgaact gaactatttg agcgaacttt tgaaaatgca gtttgacgac
      541 gaccgggaaa aatttgctgc ggctatcaca tacggcgacc atacaaggga cttgcaggac
      601 attataaacc ttgcacaaaa ccttgactgt tactggattt atccgtccgt aaaaaccgag
      661 gaagattacg ggcattatct gattgaagaa ctggacgagt tggagctgcc ggaagaagca
      721 aaaaattatt tcatgtatga ggaatacggg cgggacgctg ctatcaatga cgggggcaga
      781 tttaccgagc agggatatat ctacaacaac cgcaacacct ttacacagtg gtatgacggg
      841 cgcgacgtgc cggaggaata ccgggtaacg ccgcagccgc aggaacaggc agaccttgac
      901 gctgccgcag ccataccgac cactgccaca gagcagcccc cggttctccc gattatccta
      961 tcttccgaaa agcccgccga caaaatgaaa gagattaccg accgactgga acagggcatt
     1021 ttggggattt atgaaagcga ccgttacgcc gactatctgc gtactatgtc taaattccat
     1081 gattacagcc taaacaatac aatccttatc gccatgcagg gcggcaacct tgtaaaaggc
     1141 tataaacaat gggaaaagga atttgaccgc catgtaaagc cgggagaaaa agctatcaag
     1201 atacttgcgc ccgcgccctt taccgttaag aaacaggtgg aaaaaatcga cccggacacg
     1261 caaaagcctg ttttcgataa gaacgggaaa gccgttaccg aggaaaagga aatccaaatc
     1321 cccgcctttc gtgtggtatc tgtttttgac gtgtcgcaga ccgagggtaa ggagttgccc
     1381 gaccttggga taaaggagct tacgggcgac gtggaacagt atcaggattt tttcgctgcc
     1441 cttgaaagga cttccccgtt tgctatggga tttgaagcac taagcggcgg cgtaaaagga
     1501 cgctgcaatt atgaggaaaa gcgtatctct atcaacgagg gtatggacga attgcagaac
     1561 atcaagaccg caatccatga aatcgcccat gcaacactcc atgacataga caaagacgcg
     1621 ccggaacgtc ccgaccgccg cacccgcgag gtacaggcgg aaagcgttgc ctatgcggtc
     1681 tgtcaacatt acgggcttga tacgtccgac tattcttttg ggtatatcgc cgggtggagc
     1741 agcggaaaag aacttgcaga actgaaaggc tctcttgaaa caatccgcag caccgccgca
     1801 agtctgattg ataccataga cggacatttt gcagaaatcc aaaaggcaca ggataaggaa
     1861 cagaccaccg aacaggcaca gaccccacag gaagctacca tacagcccga agcagaagca
     1921 gccgcgccgg agcttcccga agaaacagcc ccggtacagg aaaaagaagc acagaccgag
     1981 ccggaagctg atacaggcgc aaacagcgaa gcaccgcaga ctacacagcc gggacaggca
     2041 gcccccgccg caccttatta cacaatcaat gaagctgccg ccaaacgtgc aaaggatatg
     2101 aacagctttt ctgattataa gcaaggcagc gcgacggctg aatacaggca ctatgtagat
     2161 gaagccgtac agcttgcaga aaggcagaaa caacgggtag acccgatgta ccatgagaaa
     2221 atcgacagcc tgcttgacac ttacgcccgg aaactggcgg cgaacatgaa caaaggctat
     2281 gagattgacg cgcgtgttcc ctctatcctc attgcgggcg gctctaactt ccccacaagg
     2341 aagaaagaaa agcagaacgc agcccgcgac agcaattacc gggaatggca ggacatacaa
     2401 ggacttcttg ataaaatccg cagcaccggc atgggcggta tcagcgcaga cgacccgcag
     2461 gcagtacaga agttggagaa gaaactggaa aaccttgaaa aatcgcagga aaccatgaaa
     2521 gccgtaaacg cctactaccg caagcataag acccttgacg gctgcccgca tttgtcgccg
     2581 gaacagcttg aaaaactgaa agcagacatg gcgagcagtt ggcatttggg ggacaagcct
     2641 tttgcgactt gggcgttatc caacaacagc gcagaaatca ggcgcgtaaa agaccgtatc
     2701 aaatcccttt cacagcaaaa ggaaatcggt tttgtgggtt gggaatttga cggcggcaag
     2761 gtggaagcaa acaccgaagc gaaccgtctg caaatcttct ttgaagataa gccggacgaa
     2821 gccacgcggg aagccttaaa aagtaatggc ttccgttggt cgcccaaagc aggggcatgg
     2881 cagcggcagc ttaccagtaa cgcctactat gcggctgatt atatcaaggc gattgcaccc
     2941 tttaccgggg aaaagcctac cgagatacag cgggcgcata tccgggcgca aaaagaagcg
     3001 gcgcagaaaa agcccgaaca ggaagccatt tacaaggtac acgcaaatcc ccgcagcgac
     3061 agcagggaca acctgtatct gctgcaagcc tacattccgc aggaggacgg gaaagcaaag
     3121 ataggcgacg ttttgtatat cggaacgccg gagaaatgcc gagagcttat ggcgcagctt
     3181 accgccgggg agctgacaca gggagaagtc aaggagcttt acgcaaagac gcagaccgcc
     3241 gcgccggaaa tccctatgcc agaccctaca atcagcgtca aggatatgca ggaatacggc
     3301 tatgcttggg acggtatgct tccgcttcag caggaagccg ctgaacgcct tttccatgag
     3361 gatatgtccg tttatcttct ctatgaggac ggcacagagg ggcttgttga taccttggag
     3421 gatttacagg cacacgcaca aaaaggcggc ttgttcggcg tggaaaagga aacatgggaa
     3481 gccctgcggg agtacaacgc catgaagcaa gaactacggg aaagcgagcc atcaagggaa
     3541 gctctcttac tctatgggaa cgaggacact ttcggaattt atcagctaaa gaacggcaaa
     3601 gaaacgcggg acttgcgctt tgagccttac gaccgcatac aggcggcggg aaacagcatt
     3661 gacagaggca attatgagct tatctatacc gcacccctta cgccgggaat gtcccttgaa
     3721 gatatttaca cccgcttcaa tatcgaccac ccgaaagatt ttaagggaca cagcctttcc
     3781 gtttccgacg tggtggtgct tcatcagaac gggcaggaca ccgcccacta tgtagacagc
     3841 ttggggtata aggacgtgcc agagtttttg cagccgaaaa actatttgaa agctgccgag
     3901 cagaccaccg agcaaaatta caacatgatt gacgggcaga taaacaacac cccgaccgct
     3961 gcggaactgg aagaaaaagc aaaagccgga ggacagattt cccttgcaga atacgccgaa
     4021 gcattgaaaa ccgataagga acgtggcaag cctgccaaac aggaaaagcc ctctatccgg
     4081 gcgcagctta aagcagcgaa agaacagaca ccaaagaaac aggcaagaca gaaaacacag
     4141 gatttggaaa ggagctgacc tatgaagtta caaaaatttg aaaatgtgga cgttatcgct
     4201 tcccttgaag ccataatgaa acagaatacc gctttttatc agagcgattt tgacatagac
     4261 aaacagattt tgcagaaagc ggcggtaagc cctgtcccgg aggacaaacg gcttttgtgg
     4321 ttttcccgcc cgtccgggac gtgctgcttc cgggaacggg acgtttttct aaaggacaca
     4381 aggcagcata atacatggcg gttttacggg gaacagaccc gcgatactat ccttgcctat
     4441 gcggtggaac tgacaggcac agagcaggaa aaaatcaagg gcaatcttta tgaactggat
     4501 tatttgcagc atttccggga agtaatcgaa aaatcaatcc ctgccgacaa ctacacgctg
     4561 atttatgagc atggggagct gacaaaacct gccggacaat attttgacgg gaacactgac
     4621 ccgcagcttg gaaagtttat acgttttgaa gcacagccaa acgacccgga agccttaaaa
     4681 agcattttga gggaacagca gaaaaaacga acaccgcata cgcaggggga ttttcagtta
     4741 catatcgccg ccctgcatga cagacggatt gaagcagaag cccgccggat tgtggaaaat
     4801 gtaaaagggc ttggagaacc ggacagcccc gaaaagaccc attgtgcggt agaactttct
     4861 ccctactttg ttccactggc aacggataag gatatggaac ggctgctttc catgctccct
     4921 tacaaaagcc tttccttttc tacgttggag ggcaggcatg gcgtttatgc gctgatagat
     4981 aaaagcgaga ggaataaaga gatgaaaaag tcccgtccct ctgtccgggc gcagttacaa
     5041 acagaaaaat ccccaaaact cccgaaaaag acagcaaaat caaaacatca cgaattggag
     5101 gtatgagcat gattagactg accgttgaag aaatgaacct gttgagtatt taccatgagg
     5161 gaagcaaggc acagcttatg gaaaatatga cgactgcgct accattcatg gacgaggata
     5221 tgcgtccctt tgcagaacgc accttacaga aaatttcccc actaacagaa aacgagtatg
     5281 cggagctttc cattttcgcc gctgatgaag tatgaccggg agtaagcggc tcacgccgcc
     5341 acagagccgg aaagtcaaca gccttgtaaa aaaggagtgc tgcaactgcg agcgcggaca
     5401 ctgtatctta ttggacgacg gggaggaatg tgtctgcccg cagcttattt cctattcact
     5461 tctatgcaag tggtttcaga ccgccgtact tccccttgat aagttgctct atgcagagct
     5521 tttcaagacc gaggataaaa agcgttgtac cgagtgcggc acgttctttg tatcaaaatc
     5581 aaacagcgtc aaatactgcc cggactgccg gaaacggatt acccgcagac aggcagcgga
     5641 acgcatgagg aaacgacgcg cggcggttac gcaataaggg cgaaaaaagc cttgatttac
     5701 agggcataaa cggcgtgaaa ctggcatagg cgatacagaa taccttttcc cttgaaatcg
     5761 gggttctact gcgtaacatt ctaaaatcag tacctataaa agaacagggt gcgggaggtc
     5821 attactggct tcctgcaccc tgttcttttt gtgtctgact gtctttatcc tgcatttccg
     5881 gcttcggctg taattctttt tctatccggt tccggtaatg ggttttcctg cggtaaaaat
     5941 attttgttac ttcctgttcc ggcagccata tccccgcagc ttctatctgt tccataacag
     6001 cggcaacgat ttcccggttg ttgcgtttat gcggctcctg tcccttttct atccaaaggc
     6061 ggcggtattc ctcataaagc catgtactga tagtttcttt ttgccgttgc ttcaaatgtc
     6121 gaaactgctt atctatccgc agcagcttcc cgtctacctt tttatgattt tttgtcccga
     6181 tttctttttt cctctctttc catttcttca aggggaaatt gggggagggc ttctatcagc
     6241 ttcattgtga tatactggcg catatcttca tcaatttcca gtttgaatgt tccgtccggc
     6301 tgacgtttct ttacagttgc ctgcctgtcg atttcgtcct tgtagcacat aagcaccttt
     6361 tccaccgccc atgtttctcc ggcaacggcg gcgcgtatgg tttcataatc gggtattgtt
     6421 tggtctgcca tgttttcacg ctcctgtctg tattggaata tctttatcct accacgcttt
     6481 tatagatatt tctactgtaa gcgttcggtt tccctgctcc gttccggttc tttttccagt
     6541 cttaaaatac tgtctatgtt ctgtttgata acgtcgtatt ccttgacctg ttttttcagc
     6601 ttgccataat cagtgtaaag ggcttctttc tgcgcttgga gcttcccgta ttcttcctgc
     6661 atggcggcga gattggggag cttggtaacg cccgacgctt tcagtgtctt tgccgccgct
     6721 tcatggagta tcagactgct ttcatttttt gcccggtatg cgtctttatc ctttgccctg
     6781 cggtatgctt catagactgg ttttgtcttt tgataggtgg taacgtgctt catcagcacc
     6841 gccatatctg caagacgctt ttccacactc tttaatgcgt ctgcggtctt ttcgttttcc
     6901 gcagttattt cttcaatccg gctgactaaa tccgcgtact gctcaatctg atgttctgtc
     6961 aagaaattca tggtctttgc cgccagtttc agattatgga ttttcgccca ctgttcatag
     7021 ccccggctct cttgtgcctt gatactgttt tcaatgtcga taagcagcga tacgccacgc
     7081 tgctctttgg gagctttcgc tgcctttgtg cgcttgcctg caatccgttc tttgattgct
     7141 tcctctgtat aatctgcacc gagggttttt aagcgggtaa accgttcctg tccgggagcg
     7201 cggcaggaga tatattttcc cggctttatc tcatagcccg ctgcctgtaa ccgctgcaac
     7261 aattcttcaa aacttgctac ttgggggata agcgtatcaa cggcaatttt tagcttgcct
     7321 ttccaacttg taccgacctt ttccgcttga tattctgcat agctttttcc cttgctgcct
     7381 ttgcccggaa cgacgacgga cagcccgttt tcccgacaca gcttgtcgct catattccgt
     7441 atgccgtagt agctgcgctt attggaattg tacttatggt agtcaacaaa gttgaccgcg
     7501 caaaaaatga tgtggttatg gatatgcccc ttgtctatgt gggtggtaag gacgtattcg
     7561 tgctgtccct ttgttgctgc gtcggcaagc tgctttccga ttgcatgggc tttctcacag
     7621 tccacctctc ccggttcaaa cgattgtatc agatgaaagg ctaagttgtt ccctttctgc
     7681 cgagcctgcg acaaggtata ttcaaactca atatctgccg tttcatagct gcacccaaaa
     7741 gaggacacaa gcgttttccc gtctgtctta tccggatttt ggatataatc aagggctttg
     7801 ctcaacgtgc ttttaacagg cttaatcttt gtaaccgcca tatctctgca agcaccccct
     7861 ttatttcctc tatgtcctct tggtagacgc tgcctgtcgc attgacacgc tttgcaatct
     7921 gattgatgtt gaccccgatt ttctgtatct ccgctgtcat ggcttttata tcgctatggt
     7981 ctgtctgaat gatatagccg tcaatggcaa tcttgcgaag atacgccgcc atgttttggg
     8041 tagggacgag cttcattttc tgctcaatca acaccctttc ttcctctgtt acatagaatt
     8101 tgatttgtac ttttcttttg cgtccgttca tgcaatcgct cctttccgct ttgagggttt
     8161 gggacacttc ccaacaagca attttcaacc gacgcaccgc aggacgggag ggcgaaaata
     8221 cggaaaaggg tactcttttc ctatgcttgc tacgaaagtt ttttcctcac tggttactac
     8281 aacgcaatgc tctatatgcc aaaaaagagc aaaagaaaaa cgctgcaatc tgtaaagatt
     8341 acaacgcttc ctaaatcata ttcaatttgc cggacacatt tatttgtcct ctttttcgac
     8401 ttctgaaatt tcttttgcag ttgccgttac aatccgtatg ccattatcgc tcatatcatc
     8461 aaggagagcg tcgagctgcc gccgctttgt atctttttgg gtgggcgtat ctaaaagaaa
     8521 ctggtctacg gatatattgt aacgaagcat aagctcaaaa aagatttgca gactggggtg
     8581 ctgtcctttg ttttcgatat ttgcaagata gcgaggagaa atatacattt catcacttac
     8641 ctgtttgcgg ctttctttgc gccccgtccg cgcagctttt atagcttctc caaatgcctt
     8701 gaagtcatat ttttctaccg gtctttttgc catatttatt cacccagtta cattttactg
     8761 ttcccctttc tctttggata tgtctataca gttccactaa atagccataa taattcatat
     8821 ttatatactt gctattgttc gtgtgtccga ctataattat tatgataaga ttttcagaaa
     8881 ggacgggtgt tgttatggaa tatatgtctt gccccgaagc agcaaagaag tggggaattt
     8941 ctgaaaggcg ggtacagata ctttgcaggg agaaacgcat acagggtgtt tcaaaacttg
     9001 ggtatatgtg gttgatacca aaagacgcgg aaaaaccgat tgacggtaga accaaacaag
     9061 gaaaggagct ataccatgaa taaagttttg attatagatg atgataagga actttgtgcg
     9121 ctgattaaac gcagcgtgct atcagaagat atagaagctg atttttgtaa tatgggaaaa
     9181 gagggattac aaaaattaaa agagagagaa tatcaacttg tgatactgga cgttatgatg
     9241 cctggtatgg acggatttga aacattggaa gaaattagaa aagaaagcag cctgccgatt
     9301 ttgatgttta catcaaaaaa tgacagtgct tctaaagtac gcggtttaag ggcaggagct
     9361 gatgattatt tgacaaaacc ttttgacatg gacgaactga tagcccgtat cgtttccctt
     9421 atacgccgtt atacccgttt caatcaacag gacggaatat cacagcaact tgaatttgac
     9481 gggttgaaaa ttgatattga aaatcgttct gttactacgg aaaatggaac ttttgaattg
     9541 cctccaaaag aatttgactt actcttatac tgcgcgaaaa atcaagggaa aattctgaca
     9601 aagcaacaga tttatgaaga agtgtggaga gaggaatatt tctatgatga cagcaacatt
     9661 atggcgatta tcagccgact tcgcaagaaa ttagaggtaa atcccggaag tccaaaatat
     9721 attcaaacga ttaaaggtat cggttatcgt tttaacaagg aggtttgatt ttctatggaa
     9781 gcagtcattt ttctatccat aataattgca cttttctcta ttcttgtttc ctgctttgct
     9841 attcgacgtg ttaaaaaaca gatagccgag ataacagacg cattgattga tgtaaaaaac
     9901 ggaaatggaa acaggcgtat tttatctgca acaaatgaac tggtagcccc tcttgcctat
     9961 gaaataaatg agattgttgt gtcttatgag agcaggcttt ctactgtccg gcagacagaa
    10021 gaaacaaacc gacagcttat gacaagtctt tcccatgatg ttcggacacc gcttacaacg
    10081 cttattgggt atcttgacgc tgcacataaa ggaattgtta caggaaaaga cagggacgac
    10141 tatatcgaaa cagcccgccg caaagctcac gatttgaaag aatatattga tgttcttttt
    10201 gactggttca aattaaattc taatgagttt gctatggata taaatattgt tgaagctgcg
    10261 gagctgacac gaaatatctt gattgactgg atacccatat ttgaagataa gcagatagat
    10321 tacaatattg atattcccga acagcctttc cgggtaaaac ttgatacgga cggctatatg
    10381 cggatattga acaatcttat tcagaatgta atttctcaca gtcatgcgga taaaatagaa
    10441 ataatcctgt caaagcagga aaaaaatatg caaatccgat tagcagataa tggaataggg
    10501 attgaaaaag aagatttgaa gcatattttt gaacggctct ataaatgcga taagggacgg
    10561 tctgaaaaag gcagcggtct tgggctttct atcgcacacc aacttgtaga aaaaatgaac
    10621 ggcacaataa cagcaaatag catacaggga acaggaacgg aatttacttt gctttttccc
    10681 ttagcaaatt aatgttctcc cgtccttttg acgggagcct taattatttt taggacaata
    10741 tttcaaattg caaggttaat gcaaggttgc ggcaaggtta atgcaaggtt ggtatgatag
    10801 aatactttac agaataggag gtttgaatat ggacaaaaat tatatcattg aaacaaaaaa
    10861 tcttaccaaa cagtacggct cacaaaagag tgtcgctgac ttaaatattc atgtgcagaa
    10921 agggagaatt tacggactgc ttggaagaaa cggagccgga aaaacgacca ctatgaaaat
    10981 gctacttgga ttgacaaagc caacttccgg cgaagttaaa atatggggaa agtctttgca
    11041 ggggaatgaa aagaaattgt tgccccgtat cggtagtctg attgagtcgc ccggttttta
    11101 ccctaatttg actgctacgg aaaacttaca aatttttgct accttacggg gcatacccaa
    11161 ccgtcattca attaaaaaca cattggattt ggtaggattg ccttataaag acaaaaaact
    11221 gttttcccag tattctcttg gtatgaaaca acggcttgcg attgcacttg cagttatgca
    11281 cgaccctgaa cttctgattt tagatgaacc tatcaatggg cttgacccga ttggaattgc
    11341 agaagtacgt tcttttattc gcgagctttg cgatacaaag ggaaaaacaa ttttaatttc
    11401 cagtcatatc ctttcggaga tttctttgct agcagatgat attggaatta tcgaccatgg
    11461 cgcactattg gaagaagaaa gtcttgcgga actggaacaa aaaagcagca aacatatccg
    11521 atttactctt tctgataccg cacaggcagc aagaatttta gagcgtactt tccatgagag
    11581 ccatttctct atacaggacg accacaattt acgcttgcat aatcttgatt tgtccgtagg
    11641 aaaaatcgtg actgcttttg tagaaaatgg attggaggta tcagaagccc atacttgtga
    11701 agaaagtctt gaagattact tcaagcgtgt gacgggaggc gagggaattg cttaaactaa
    11761 tcaaatgcga attttggaaa ttaaaacgga aaagattatt atatgcactg atgtcgttat
    11821 cgttattttt tccgttgatt ttggcatata tggcaaaggc gggaacaggt gcagatacaa
    11881 cggaacatta tcttcaaacc cgatttgatt atgtttatac aatgatgttg ggatatggct
    11941 tagctttttt gctcccatgt ctagttggta ttattgccgc tattttgttt tttatagaaa
    12001 gagattgcga tacttccaaa aaccttagaa caattcctgt tacaaataca cagctcatta
    12061 tggcgaaaat ttctatgctc tttattttca gcattgcatt ttgtctgata agcacattat
    12121 ctgtcgcatt gttctgtaaa ctgtttcatg taggaatggt ttatggtatg gcttataaaa
    12181 tatatatgag ccttattttt ggagttctta tcgtagcagc ttctttgccg attgtagttt
    12241 taattattta ctttaacaaa agttttttgc tttctatcct gcttgcattt ttttattcta
    12301 tttttaactg gggcttaatg ggaacgattg ggacatctat aagtgctgca aaaattgctt
    12361 tcttaaattc tttcccagtt atgtgtgtta tgaactggac aagcggctta atgatggata
    12421 atttgcaaaa agataatctt ttgccggaag cctatgcgat tgtaccaact actttccata
    12481 caatttttat tatggcaatt acagttgtac tttctttgtg gctgataatt cgcttttata
    12541 aaaaatggac gaggtaaaaa atatgagaaa tataataaaa actgaatttt taaaattaaa
    12601 acgatattct gtaataaaag ccggaattat tatgacaaca ctttctccgt tgctgtcact
    12661 gttttattcc acagccaacg gtggaccgac atggacgttt gactatttta tgcaacaggt
    12721 tatgattagt aattgtacct tattttttcc gattattatt gcactgattg caggttatat
    12781 cataacaagg gaatatacag acgatactat gaagaatata ttaacaattc ctattccata
    12841 taaacagtta ctttctggaa aattacttat tctattgctg ttgacaatca gctttagtct
    12901 tataggttgt gtgatagcat tagtaatcaa tattattgtc ggatttccgg gagttcattt
    12961 tggcaatcta ctcaatatgt ttattcgtgt tactggggcg aatataggaa tttatatttc
    13021 agtcttgccg attattctga tattctgttg ttcggcaaat aattttttgg gtggagttgc
    13081 actagctttt gtgtatggat attttggaac ttttgaagga acactgttaa attattatcc
    13141 gattaaagca agcatgattt tagtagaccc tacttgtggt gcggaatatg gttatactta
    13201 tcacattttc cccgctttca ttacaatagt tttgactttc ttgatttcta tgattattct
    13261 tgcaaataaa aagaaagaac ctggtacatt gactgttggt aagaagaaaa aagcagttag
    13321 aaaaaagggt tggtaaataa tttgattgat agtacctgcc gaacgacggc aaaagaaaaa
    13381 aagctgtcgc agagcagata cactttcacg cgcctttgaa gcggtggctt tgatttttag
    13441 agccgccgct ttttttgtct actgctaaat cacgacaacc tataacataa attagcggca
    13501 tttaggaggg ctgccgctat ggggatttct ttgttgctat acagcataca taggttacat
    13561 tcattaaaaa aagtatagct cccccaccgg acaagtccga ctatacagat gaactatcaa
    13621 atcaattaaa tactgctgtt atttcctatg cgtcggtctg cgccttgcag atgggcgcat
    13681 tttgcatttt acagaagatt tttcaaaaac tttgctctct ttttggcaaa gcaaaaagtc
    13741 gcctgtatta tcccgcgaaa aggggaggta ctaccgcctt tcggcagaaa ggaggtgatt
    13801 acatggaacc taatcgcaga gaatttcaga agcaatgtgt ctttcaaagt ttctgtaaac
    13861 gggtgttaca caatgaagcc tgtaacgccc atgatgaaat ccggcgacgc aggaaacggg
    13921 aagtttcttt ttgtgacctt gccttgcatg aggaacggca gctttacact acggataaat
    13981 attttcaagc tgaaacagcc gagccgactt atcaaatggc gggaaaagaa ataaccccga
    14041 agttattgct tgaagcaata cgcaccctgc cggaagaaaa gagaactgct atacttctgt
    14101 actatttcga gggcatgact gacgtggaaa tcggaaagat gttcaatacg tcgcgcagca
    14161 cgatacagta cagacggaca agctcttttg aactgttaaa gaaatatttg gaggaacacg
    14221 ctgatgaatg gaacgaatgg taatcaaaac gactacctgg aaaatgccct tgttccctat
    14281 cccgttattt tggcggcgag caaaggcgac ccggacgcta tgaaaatggt cttgcagcat
    14341 ttcagcggct acatagcaag tctttccatg cggaagctct acgacgagcg cggcaatgtc
    14401 tattatggcg ttgacgagga tattcgggaa cggctgcaag caagactgat gagggctatc
    14461 ctcacttttc gggctgaata acctatgcga tattggaacg cttctttccc cttttccgtt
    14521 ccatatcgca gaacagccct ctgctctttg ataaagaaag cggcgcaata gaacggcgac
    14581 gcagtataag gcaaatcgga tacgttcctt ttgtgccgag ccatgcggcg ggtacgccat
    14641 gacctttctc ttaatagaaa agcgagcgag aaataccaca ccgtaaagca gagatagcag
    14701 cctgcgggcg ataacgcaca gaatacataa tgatacttcc ttatagccat agtccgagcg
    14761 ttaggagcgt cgcaggcaat gggtagggct tcggcagacc gccggagggg tgcaagtccc
    14821 atgaggttac gctaatagcc gtccgattaa agcctatgtt acagattgat ttttgaccca
    14881 tt
//
LOCUS       DACXMZ010000083        14823 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.083, whole genome shotgun sequence.
ACCESSION   DACXMZ010000083 DACXMZ010000000
VERSION     DACXMZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14823
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.083"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            110..1900
                     /locus_tag="JD016_10000"
     CDS             110..1900
                     /locus_tag="JD016_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAU4999966.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            1912..3084
                     /gene="rpoD"
                     /locus_tag="JD016_10005"
     CDS             1912..3084
                     /gene="rpoD"
                     /locus_tag="JD016_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAU4999967.1"
                     /translation="MSVENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETEL
                     DKDQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAI
                     EIETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEA
                     NLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAIT
                     RAIADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIM
                     KIAQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQ
                     KVLKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            3249..3938
                     /locus_tag="JD016_10010"
     CDS             3249..3938
                     /locus_tag="JD016_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAU4999968.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            3952..5049
                     /locus_tag="JD016_10015"
     CDS             3952..5049
                     /locus_tag="JD016_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAU4999969.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            5109..5684
                     /locus_tag="JD016_10020"
     CDS             5109..5684
                     /locus_tag="JD016_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAU4999970.1"
                     /translation="MMKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAK
                     TLPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFS
                     DIWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAY
                     GILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(5744..6211)
                     /locus_tag="JD016_10025"
     CDS             complement(5744..6211)
                     /locus_tag="JD016_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HAU4999971.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(6597..7073)
                     /locus_tag="JD016_10030"
     CDS             complement(6597..7073)
                     /locus_tag="JD016_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAU4999972.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(7193..8284)
                     /locus_tag="JD016_10035"
     CDS             complement(7193..8284)
                     /locus_tag="JD016_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HAU4999973.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKKFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(8277..8666)
                     /locus_tag="JD016_10040"
     CDS             complement(8277..8666)
                     /locus_tag="JD016_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU4999974.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            8858..9304
                     /locus_tag="JD016_10045"
     CDS             8858..9304
                     /locus_tag="JD016_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999975.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            9589..10293
                     /locus_tag="JD016_10050"
     CDS             9589..10293
                     /locus_tag="JD016_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU4999976.1"
                     /translation="MLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSGKEMISSN
                     EYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGGDDYITKP
                     FRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFLTSVEYKL
                     LLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCEPIYIETV
                     RGIGYKWIGSENSVSI"
     gene            10271..11524
                     /locus_tag="JD016_10055"
     CDS             10271..11524
                     /locus_tag="JD016_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU4999977.1"
                     /translation="MKIVFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMV
                     VRNNQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPI
                     ISKLVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKQFDMKN
                     KNQEGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLND
                     LLYNDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHR
                     AMLSMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITY
                     KENPLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNG
                     DIYVNSEKGKGTEFHIIFHKMYDSD"
     gene            11604..12281
                     /locus_tag="JD016_10060"
     CDS             11604..12281
                     /locus_tag="JD016_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU4999978.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            12282..>14823
                     /locus_tag="JD016_10065"
     CDS             12282..>14823
                     /locus_tag="JD016_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU4999979.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIVCTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDN"
BASE COUNT         6067 a         1527 c         2480 g         4749 t
ORIGIN      
        1 aggatatcaa taaaaaaagt cgaattgtgt atattgagtg attgacaaat ttagaatata
       61 gtgtagaata gaaatgttaa cacaaaaaat tacaccagaa ggtgataata tgaatgatat
      121 aaaagacatt attgaagaaa taaaagctag atgcgatata gcaagtataa tatctgaata
      181 tatgaatata aaacaatctg gagctaatta caaaggatta tgtccatttc atggcgaaaa
      241 gacaccttct ttttatataa atacgtcaaa acagatttat aagtgttttg gttgtggtga
      301 aggtggagac gtaataaatt ttgtcatgaa aatggaaaat ttggatttta tggatgctgt
      361 aaaaatttta gcaaataaat gcggtataga aattaacact aacatgaatg aagaaactag
      421 aattaaaata gaaaaatcta agaaatttca agatattcat acggaagcag ctagatttta
      481 tctttcaaac ttattgggaa gcaaaaatct tgggtatgaa tatttaagaa taagaggact
      541 agatgataaa attattaaga aatttggttt aggattttct ttggattcat ggaattcact
      601 tatgaatact ctaatcagta aaggatataa aaaacaagac ttattagagt gcggtctaat
      661 tgcaaaaaat agagatggaa ctaattgcta tgataaattt agaaatagag ttatgtttcc
      721 tatattcgat tatagaggaa atataatagg atttggggga agagtattag acgattcttt
      781 gccaaaatac ttaaattctc cagacacatt aatttttaat aaaaaacaga acttatacgg
      841 attaaatttt gctagaaaaa atttagaaag taaaactata gtcttagttg aaggatatat
      901 ggatttaatt tcgctttatc agtatggtat aaaaaatgta gtagcaaccc ttggaacagc
      961 cttgactgaa caacaaggca ttttaataaa gagatatgcg gacactgcta ttatatctta
     1021 tgattctgat gaagcaggaa taaaagcgac tttaagagct atagatatac ttactaaatt
     1081 aggtatcaat gtaaaagtgt tagacttaaa agatgctaaa gacccagatg aatttgtaag
     1141 aaaatacgga cttagtgatt ataaaaaagc tatggatgtt tctactcact acataaaata
     1201 taaaatagat catcttaaaa aagaatttaa cattcaaaaa gatgaagaac gagtgaagtt
     1261 cgcaaaagaa gcttctaaaa ttataaaaca attgacaagt cctgttgaaa ttgattttta
     1321 tacaaagtat ttaagcaatc aaatagatat taatgtcgag tctataaaga gagaagttta
     1381 tggtaaaaat tacaataagc catataataa caaaaatcaa aagaaaatag aagaaaaagt
     1441 aattgaaaaa gtcgaagtca gacaagacgg aaaacaatta gttgaagaaa ctctaataaa
     1501 aataatgtta gaagacaaaa aaattagaga aatagcgtta cttaaagtag aagaaagtga
     1561 ttttttattg aaagaaagta aagaaatttt aaattacatg attaaaaatc aagaattgga
     1621 caaaataact attgacaaat tgaaaagttt aaacatatcc gaagaatact taaaagaatt
     1681 aaattcaatt tctttgaata gtataaactt agagaacaca aaagaaatag aaggaataat
     1741 aacaaatatc agaaaaaact cattagaaga gcaaattaat agcttgttga gagaacaaca
     1801 agaattagaa aataataatg atatgaaaga ggtagatggt agagttatgg aaattgcttt
     1861 aaaaatagtt gagataaata aaattttaaa gagcttgtag agaaaggagg tttgagtgtg
     1921 gaaaataaat cgaataaaaa agagttaaaa aaggttactg ctaagacatt aatagaaaaa
     1981 ggaaaaaagc aaggttctct gacacttgca gagataatgg aagctttttc ggagactgaa
     2041 cttgataagg atcaagtaga aaatctttat gagactttag gaaatttggg aatagaaata
     2101 acagaaacaa aaaactataa agctgatata gatttttcgg ttgctgatga cgatttaagt
     2161 ataggccact tagatgaaga tgcagaggca atttcacatg atgactcttc tgcaatagaa
     2221 atagaaactg tggatttatc tttaccaaaa gggataagta tagatgaccc tgtaagaatg
     2281 tacttaaaag aaataggaaa aattcctcta cttaaaccac atgaagaagt agaatttgct
     2341 agaaggatgc acgaaggtga tgagatagca aaacaaaggt tagttgaagc aaacttaaga
     2401 ctagttgtaa gtatagcaaa aagatatgta ggaagaggta tgcttttctt ggatttaata
     2461 caagagggaa atttaggtct tataaaagca gtcgaaaaat ttgactatac aaaaggatat
     2521 aagtttagta cttatgcaac atggtggata agacaagcta taacacgtgc tattgcagac
     2581 caagctagaa ctataagaat accagttcat atggtagaga ctataaataa gctaataaga
     2641 gtatcaagac aactacttca agaacttgga agagatccaa aaccagaaga aattgcaaaa
     2701 gaaatggaaa tgacagaaga taaagtaaga gaaataatga aaatagctca agaccctgtg
     2761 tctttagaaa caccaatagg agaagaagaa gatagccatt taggtgactt tattccagat
     2821 gatgatgctc cagccccagc agaggcagca gcatattctt tattaaaaga acaaatagaa
     2881 gatgtacttg gttcattaaa tgatagagaa caaaaagtat taaagcttag atttggtctt
     2941 gaggatggta gagccagaac tcttgaggaa gttggaaaag agtttgatgt aactagagag
     3001 agaataagac agatagaagc aaaagcacta agaaaactaa gacatccaag tagatcaaaa
     3061 aaacttagag attatttaga ctaaaaagat acatcattga tgtttttgtg ataaatgcca
     3121 tgagataagt gatatctcat ggctattttg tagtaaagat ttaattttat ttttgtagtt
     3181 atttcagtct aatatctata acttggatta aatcctataa ataaagggaa ttgaaaggag
     3241 aattatattt gaaattaaca gatagattat tgaaaatagc atcattagtg tcagatggta
     3301 aaaaaatagc tgatataggg acggaccacg gatacatacc agtatatttg ttaaaagagg
     3361 gaaaagtacc atttgcagtg ctagcggatg ttaataaagg acctcttgac aatgcacata
     3421 aagaagtcat acagaacaat cttttagata aagttgattt acgattgggt tctggtatag
     3481 agatacttga aataggggaa gtagaagaag tgataattgc tggtatgggt ggaatcttga
     3541 taagtgagct tctcgaggct aaaaaagaag tagcacataa tgtagaaaaa ttaatattac
     3601 agccaatgca agcacaagaa gaacttagat attacctttt aaacaacgga tatgaaattt
     3661 tagaagaagt tttagtgaga gaagatttta gaatatatga gataattgtt gccaaatata
     3721 caggtaaaaa tactataata gaagatgaga tatattatga ggttggaata aagcttttag
     3781 aaaacaaaga ttctcttttt aatgatttta tagaaaaaaa gataaagaca tatagttcta
     3841 tagttaataa attagaaggg aaaaatgggg aagctataga taagaagaga aaggaaagcg
     3901 aagttgcaat aaaaaaactt gaaaatttaa taaaataaat aggaggtata tatgctatta
     3961 aagagtctaa ctagaaaaat tgaaaaaaag tatcctctaa acttagcaga ggattgggat
     4021 aatgtaggtc ttatagttgg agattttgat atggatgtaa aaaaggtatt ggtatcttta
     4081 gaagctaatg aagatgttat tgatgaagct atatccaaaa atatagattt aattgtgaca
     4141 catcatcctt ttatatttgg gaaaattaat aaaattaata gtggagattt aaaaggcaga
     4201 cttatccaaa aacttattaa aaatgatata tcactttatt ctatgcatac gaattttgat
     4261 atagcatttg atggtctaaa tgactatttt atggaaatta tggaatttgg taattcaaag
     4321 gttttagata taactaaaag cgaaaatttg tataaattag cagtatacgt tccacataac
     4381 tattcagatg aacttagaaa ggttttaagt aattcaggag caggtcatat agggaattat
     4441 agtgattgta cgttttcaat agaaggtgaa ggacaattta agccattaga aggctctaat
     4501 ccgtttcttg gaagtgtaaa tgatatagaa actgtaaatg aagttaaaat tgaaactgta
     4561 gtaccacaga agcttttagg tggggtaata agttctatgt tagatgcaca tccttatgaa
     4621 gaagttgcat atgatttgta caagttagaa aataaaggtg agatatttgg actaggtaga
     4681 ataagtaaac ttgataaaag tatgacatta gaaagtcttt caaggaaaat aaaagagaaa
     4741 ttaaatatga aacacataag agtagttgga aaccttagta cagacataac gaaagtagcc
     4801 gttgtaacag gagctggttc agaatttgta aaaaaagcta agagacaagg tgcagaagta
     4861 ctaattacgg gtgatgtaaa gtatcatgag gcacaagatg cacttgatat tggaatgtgt
     4921 atagtggatt gtggtcattt tgatacagaa gatatattca aaaatgttat gaagagattt
     4981 ttagatgaat ttagtgaaat tgaagtaata aagagtaatg tatatttgaa tccatttagt
     5041 actatttagc agtgtttgat aataaaaata ctatataaaa aataaatttg agtttaatta
     5101 gggggataat gatgaaagta gcagtaattt ttcatagtgt ttgtgggagt acatatttat
     5161 tagctagaga atataaagaa gcgcttgaag aaatgaatat cgaggtagat atatttagag
     5221 taagtgacga agttgcaaaa acacttcctc aatattattt gataaattcc aaagaataca
     5281 aagacgaatt tgaaagtata aatgtaatta aatctggaaa agaaatatta gattatgatg
     5341 ctatatttat gggttcacca acatattatg gaaatgtatc tggaccaatg aagatgttta
     5401 tggatagttt ttctgatatt tgggtaggtg ctcctttgag tggtaaaata tttggttgtt
     5461 ttgctacagc aggttcacaa catggaggag gagaattagc tttgcaagct atgaatatat
     5521 ttgcccaaca tatgggaatg actcttttgt cagttccatg ttcagtaaga ggaggttatc
     5581 cagcgtatgg aatacttcat atagcaggag ataattctga tataagacca aatgatgatg
     5641 ccaagatagg tataagagat tacctaaaaa gacttaacat ataagaatat catataaaaa
     5701 tacctaaaag cgactgtata tacttttagg tatttttact taattataaa cgtgaagctt
     5761 taccagaaat atggtcaatt tctattttaa caacagttgt taagttcata tccttctcta
     5821 tgtacttcat gccttcttca aagaatcctt tagaatattt ttttattatt tctacaagag
     5881 catttttctt ttcttcattt tctacagtgc aagcttttcc aaaaactata gcactagaat
     5941 aagtagtact aaatttatct ggaataacac tttcattagc aactactaaa aaagaaactt
     6001 tgttgttttt cgatatatta tctaacttat gcccatttct tgcacaatga aagtaaatag
     6061 aatcattaaa gtatacataa tttacagcaa ctccataagg ataaccattt tcagatatag
     6121 tggaaaaagt accaaattca ccatttttta atacttcaac agtatcttct tttgtcattt
     6181 ctcttttttt caatctcatt tctttaaaca taatatcatc ctttctaatt ttgatgctta
     6241 aattaaattt tagaacattt ataaagatta tgctatgttt tttacaaatt ttaagaaaaa
     6301 aatatagttt aagataaaag gtagtgaaag agaagctatt tggaggttta tttatgatat
     6361 tattgttaga gtagtacaaa taatactaat aaatttaata gctggtttct tgttatttaa
     6421 ggtagtaagc atgtctataa gaaggggaag ttatcgaggg tagaagttgt tgtttataac
     6481 gatattataa agaaaatatt attatataaa ataataaata gtaaagaggt atcaagttga
     6541 atataatact taatacctct tcaaaattaa gttataaaag tgttgtggga attgtcctat
     6601 acatggtaag gagataataa agaatgtatc tttaaagtct caactaaaca gcaaaattta
     6661 tccaacaaac atacaataaa tgcttctttg tatctaggtg gcttaacagt aagtggccac
     6721 atgtgtttta aaattatgtc cgtttctttt tcatttattt gaaaaaataa agttgcattt
     6781 tttaatgctt ctctagggtg agtaaatcca tgtaatcctt ttctatcacc tttgtaatgc
     6841 caatcataaa gaaaaaagtc gtgtagtaaa gctcctctaa taacactttg aatatcaaca
     6901 cttaagtgca attttttaca taatagataa ctataatatg ctactgacaa actatgttca
     6961 agacatgaaa cattgccatg atgaggaaat gttctcataa gttgaacttt ttcagaagaa
     7021 agtatctcag aagcacattc attaaaatat ttgatttcag cttttgacaa cattatgaaa
     7081 atctccattc ttagtaatat ataaatacta tacttcaacg ataatatgaa atatttctta
     7141 tcctacaaaa tataataccc aataatacaa gtaagatacc ttttatggta ttttaaagta
     7201 agagagattt tattaggcta aacaatgaaa aaattaaaag taaagctata gcaatatata
     7261 gtatttttcc aagaggattg ccaaaattta tagtccatcc aataccaaac cttttattaa
     7321 ccattaatga tgggtcattt ggattattat acatgatgcc tgctatccaa tacttctcat
     7381 cttcttctgg agaataagat gagttgaatt ttaaatttgg ggaattaata tatgttgcta
     7441 ttaaataaat tataactaaa agcgttgtaa tattcatagc agttgatagt tttgttttta
     7501 ttgataaaag agtagtaaaa aactgtgttg tcattataat catcaatatt aaaaacaaat
     7561 aaccaatttt atttaaatat ttaatgtttt caactctact ttcttcaatt ctatttgtat
     7621 ctatcttaat ccttgacttt atggatccaa tggaagttat atataataaa accattaaac
     7681 aaaagtcagt accgattaat ttaaatacat taagaaatga tttatctata aaaacatcag
     7741 gagacccatt tatgttccaa tgagtggcaa ttgagtctgg caattgatta tagttaaatg
     7801 ctataaaaat actcattccc aaagtaaata gtatcggtat taaatagatt attttaaatt
     7861 tttttattat tttatttttc ttatttataa aatccatatc tactattaat tttgaatcta
     7921 tatctatatg acctaattta gcataaattt ctgatttaag cttcttagct tttttatgtg
     7981 tatgaatata tagtatactt tcatataata aaaaacctat aataccaata aaataggaaa
     8041 gttccaattt attaaatgta aaaaccagaa taaaaactaa tataaagtct ataataaaac
     8101 ctaaagataa tagtttttta aattttttat ctaactttat aaagtaatta taatttttat
     8161 gctcttggtc tatagaaact ccatagatgt gttttttacc tacaaaatag ggcataaaat
     8221 aaaatgtagc atacattaaa attagaactg gaaatattgt aaatagacat tcaatcttac
     8281 tcataatatt tcacctcata cttgtcaaat aaacttgaac ttagtgacaa aaattcttct
     8341 ttactaatac ctgataaata agattttgcc accaataatt ccaagtcaag tttatttttt
     8401 tgacgagttg tattatctat tttcattggt agttgagcta caatagcacc ttttcttcta
     8461 tcaatattta agaatccctc ttcttttaat tcattgtagg atttgttcac tgtatgtaaa
     8521 tttacaccaa tattttcagc catacttctt acagatggaa gtgattcatt tattttcaat
     8581 tctcctgaag ctatagcctt agttatttct tctttaattt gtacataaat agatttatca
     8641 ctattaaaat ctaaatttaa aaccataaaa aacctccatt atgctttata aatgaaaacg
     8701 attgttatat acatagtata acataaaaaa taataacaaa cacaaaatat aagtttatat
     8761 tttgtaaaaa ttgtaattta aaaaataaca taatttttat taaaataata cagatactat
     8821 ttatagatag aaataaaaat tagaaaggag aaaaaatatg ttaacatatt taatgaaaag
     8881 acaaatgcat aaaaaagatg atggaatgag taaaccaatg atgattacag gagctgtaat
     8941 tacaggtgta ggagcatatg ctgtatataa aatggtaaaa agccataaat ctaattctca
     9001 aatggtcaat acaaattatt atggaaatag cgattatgat gattataaat atgacgaatt
     9061 tgattatgac tgtgattgta gagacacaaa acaagattat gaatatgaag aattaaaaaa
     9121 aaaagtaaaa gaatttaatt caagaagaat aaattgtgaa aattgtccac atgtttctca
     9181 agaagaaatg atgcaatatg taaatagtgt taaagatgta aaggaagaga ttgatttaca
     9241 tgaagatgct aaaaaagata atatttacaa agaagatgaa tacaaaaaat atgaagaaga
     9301 ataagtaata ttttgtataa ctgaacaaat agtaatgtca aattatttaa tatctagttt
     9361 taatatgtaa gttgtaactt aacttgaaag aatcagtatc attctttaga tagttaagta
     9421 acaacttaca ttttattttt ataaatttta atagttcttt gtgactattt tgtaatttta
     9481 gaaatatttt agtatccagg gtaattgtgt ataataaaac ttgtatagat agatagtatg
     9541 aaattaagca gatagtttaa aattaaaatg aaaagaaagg aaatagttat gttaaagttg
     9601 ttgatagttg aagatgatag tactatatct tttggaataa agtatgcttt agaacaagaa
     9661 ggatttagta tagatatatc aaaggattta tctagtggaa aagaaatgat ttcttctaat
     9721 gaatatagca tgatactgtt ggatgttaca ttgccagatg gaactggtta tgagctttgt
     9781 caatatataa gaggattctc tcaggttcct ataatattct tgactgcttg tgatgaagaa
     9841 gtaaatattg taatgggtct cgatataggt ggagatgatt atataacaaa accttttaga
     9901 attagagaat taatatccag aataaaagct gttttaagaa gaaaaggtaa tacttcagaa
     9961 gaaaataaga aaatacttaa atttggagat ttaagtatat atacattaga agctagagta
    10021 tataaaaatg ataaagaaat atttctgaca tcagttgaat ataaacttct tttaattctt
    10081 atacagaata aaaatactgt tttaagtaga actcaaatat tggaaaaact ttgggatgta
    10141 acttatgatt ttgtaaacga caatactctt actgtttata taaaaaggct tagagaaaaa
    10201 ataggtgatg atttatgtga accaatctac atagaaactg taagggggat tggttataaa
    10261 tggataggaa gtgaaaatag tgtttctata taatccagaa gtgaagaagt ttttaagtaa
    10321 gtatgttact ttgatgttta ttgttatcat aatatctatt ggatttagca taatcaacgt
    10381 gtctttaact aaagagatgg ttgtaagaaa taatcaagct ataataggaa ctttatcaag
    10441 caagtatcca aacttagaaa gtcagatagt agacataata actcaaggga agtctatgga
    10501 gaacatagat tatggtcaga aaatattaat taagtataat tatgataaat ctattaggat
    10561 aaactctgag cctattattt ctaaattagt tttagagact atcaaaataa atataatatt
    10621 agtatgtata atatttattt tgatatttgt gttggttgtt agatatttta agtcaattta
    10681 caatgattta tcagatatga ctaagtatgt ttactatagt tcagagggta agcaatttga
    10741 tatgaaaaac aagaatcaag aagggcagat aggtcttcta aaaactgaac tcttaaagat
    10801 gacgacaatt cttaatgaaa aggttgaact tttaaaaaga gaaaagatat ttttaaacaa
    10861 tacaatttcc gatatatcac atcaattaaa aactcctatg acttctttaa taatgttgaa
    10921 tgatttatta tataatgatg taccatacga agtaaaaatc gattttttaa ataagataaa
    10981 aaatcaatta aatagaatgg attggttgat taaaagcatg cttaaattat caaaggtaga
    11041 agcaaaagtg ataaatttta aaaaagataa ggtaaaattt agtgaactca tacatagagc
    11101 aatgctatct atgaaaatac ctatggagat taaaaatcaa aaactgacta tagagggaaa
    11161 tgataatgta tcttatattg gtgatattga ttggtccgtg gaagcattag tgaatataat
    11221 caaaaattgt gtagaacata ctcctgaatt tggaaatata attataacct ataaagaaaa
    11281 tccattattt tctgaactaa taataaaaga tgagggtgaa ggtatagata aaaaagatat
    11341 accacatgta tttaaacgat tctatagagg aagaagtagt tcaaaagaag atagtgtagg
    11401 aataggtctt gcaatgtcaa aatctataat agaaagccaa aatggcgata tatatgtaaa
    11461 cagcgaaaaa ggtaagggta cagaatttca tataatattt cataaaatgt atgatagtga
    11521 ctaatctgta atatttaatt cactttatag taatagccaa aacctaatat atgaagtata
    11581 gaatataaat attggaggta gttatggaaa ttttaagagt agaaaatcta actaaaagtt
    11641 atggtaaaaa tgaaacaaaa gttgatgcta taaaaaatgt aagtttatca gttgaaaaag
    11701 gaacgtttat agcaataaca ggtccaagtg gaagtggtaa aagtacctta ttacatttat
    11761 taggaggagt tgacagacca actagtggta aggtttatat aaatgatgtt gatatatata
    11821 atttaaaaac taaggattta gcgatattta gaagaagaaa tataggtctt atatatcaat
    11881 tttacaactt aattccagtc ttaaatgtga aggaaaacat attgcttcca gcagagcttg
    11941 acaatagaga tattgataga gaatattttg atgatttaat gaaaacatta ggtctaattg
    12001 atagagaaaa gcaccttcca aaccaattat caggaggtca acaacagaga acctctatag
    12061 gacgtgcatt aattaataga ccttctataa tattagcaga tgagccaaca ggtaatttag
    12121 atagtaaaaa ttcaaaggaa atattagaat tgttaaaatt gtcagtaaaa aaatacaacc
    12181 agacactaat aatgataact catgacaaaa atatggcact tcaagctgat agaattataa
    12241 gcatagaaga tggaatcata aaaagtgatg aggtgatgta gatgaattta tatacatcac
    12301 taactattag atacttaaaa caaaataaaa gaagaaccat tgtaaccata attggtataa
    12361 tactttcaac agctttaatt tgtggtatag gaaatatatt tgaaagtttt atggattatc
    12421 aaatgagaga aactataaag aatgatggaa gtttccatgt tacattttat gatgtaaata
    12481 gaaaaaatgt ggaatatgta acgaaatcag ctgaaataga aaaacatgca tttaccaaac
    12541 aacttggata ttctaaatta gaaaatagtg aaaatgctat attaagtata aagcaatatg
    12601 ataaaaatgc aataaatgga tataaagttt ctataaaaga aggtagattt ccagctaagg
    12661 aaggagaaat tgttttaagt gaaagtatta ttaatcttat agatgataag ttaaaaatag
    12721 gagataagat aactttaaaa gtaggggata tgtttgactc aagcaagaaa aagattgata
    12781 gtatgacatt tcatgaaggt gattatatag caaatgaaaa agatagaact tttaaagttg
    12841 ttggtataat tgaaaaacct gggtttgaac gttataatgg aattgcaaca gctatagcct
    12901 actttaatcc tatgaataat tacaatgata caattaatgt atcagtagca gtaagaaatc
    12961 ctaaagatgt gtataagata agtaataaaa taagtaaaaa tatatattca aataaagata
    13021 aagaagcagt ttctgatatg ataaaataca atgaacattt attgaggtta caaggtgcta
    13081 gtaagtattc aaatataaat agctctataa agtctataat tatagcagta actattttag
    13141 taatcgtatg tacaattgct acagtgtaca attcctttag tatttctata aatgaacgaa
    13201 aaaaacagtt tgggatatta aattctatag gtgctacatc tagtcaaatt aaaagattag
    13261 tttttataga aggtattata attagtttaa ttggtatacc aataggacta atatctggaa
    13321 cagtagctat agacctattg tttaaaatta taaataagta ttttactgaa tcggttgtta
    13381 ctaagatgag tttgcagata gtgtataatc caataataat tattgttagt ataataatag
    13441 ttttgcttac aatctttata tcaattctac tacctgcaat atcggcttct aatatttctc
    13501 cccttaatgt gattaaaaat actggagagt ataaagtggg caaaatcaaa agctcaagac
    13561 ttattaaaat gatttttaaa actgaaggag tacttgcata taaaaatatt aggagaaata
    13621 gaaaaaaatt tataataact cttttttctt taatggtaag tataataata tttatatctt
    13681 ttagtggctt tacattactt ttactaagag gtgaagaaat tagaaattct caaagaaaat
    13741 atgatttgta tttaactgca aagaacacag ctaggtctgt agaggatact ataagtgaac
    13801 tagaagatat tgatgggata aaaaactttg aattggcaac aggacaatat gtgtctataa
    13861 gagtaagtga aaataaaata aataaatcca aggaagattt aatcaggaaa tattatcaaa
    13921 aatataagat tggagatagt tatgaatatg atttcagtaa taatgaatta atttttccag
    13981 gagattttgc agtaaaaaac aaaataaata acttagtaca gggctctttt aataaagaga
    14041 gagcaattga agaaaatggt gttatattag ttagaaaaag tgcatttgaa gaaaatggga
    14101 agaaaggtgt agtagaactt accaactata aagtaggaga tacagtaaat tgtgaatatc
    14161 ttgatgaaaa tgccttaagt aaaaaagtaa aggtcaagat tcttgctata actgatgaag
    14221 aacgtctagg tcttggttat caaaatatgg ggttacaatt tataacttat gatgaagttg
    14281 ctaaaaattt aaatttaaaa ttaagtaaaa gccttatatt tattgattca ggaggaagca
    14341 ttaagactaa gaaggaaata gaagctttgg caaataaaaa taattttaac ttttatgatg
    14401 aaagtgcaag caatgaaagt gaaaaacaag atttaaaggt tattaaaata tttgtatatg
    14461 gttttatagt agtgatatct ttagtgagtg ttacaaatat tctaaataca gtaagtacaa
    14521 gtataaatct aagaaaaaga gaacttgcag taatacaatc tataggggtt actcctaaag
    14581 gatttaggaa aatgatatat ttagaaagtt ttatatatgg tatactgtca ttgttgtttg
    14641 gaataccaat tagtattggt ataattctaa ttatgaataa acttatttca ggtgtaattg
    14701 aattttctcc gattataccg tggacagcca tagttatctg tatagtaagt gtttttataa
    14761 taacttttat agcagggtat ataccaatga gtaaattaaa taaagaaaat ataatagata
    14821 ata
//
LOCUS       DACXMZ010000084        14767 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.084, whole genome shotgun sequence.
ACCESSION   DACXMZ010000084 DACXMZ010000000
VERSION     DACXMZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.084"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(58..201)
                     /gene="scfA"
                     /locus_tag="JD016_10070"
     CDS             complement(58..201)
                     /gene="scfA"
                     /locus_tag="JD016_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine peptide SCIFF"
                     /protein_id="HAU4999980.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(267..647)
                     /locus_tag="JD016_10075"
     CDS             complement(267..647)
                     /locus_tag="JD016_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HAU4999981.1"
                     /translation="MRGGFFMGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDN
                     ITMVSSFITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEV
                     GMIYKLLLISAAGGIGGVLGVNFK"
     gene            complement(687..980)
                     /gene="yajC"
                     /locus_tag="JD016_10080"
     CDS             complement(687..980)
                     /gene="yajC"
                     /locus_tag="JD016_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAU4999982.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1114..2235)
                     /gene="tgt"
                     /locus_tag="JD016_10085"
     CDS             complement(1114..2235)
                     /gene="tgt"
                     /locus_tag="JD016_10085"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAU4999983.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2314..2799)
                     /locus_tag="JD016_10090"
     CDS             complement(2314..2799)
                     /locus_tag="JD016_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999984.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(2811..3836)
                     /gene="queA"
                     /locus_tag="JD016_10095"
     CDS             complement(2811..3836)
                     /gene="queA"
                     /locus_tag="JD016_10095"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAU4999985.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4154..5173)
                     /gene="ruvB"
                     /locus_tag="JD016_10100"
     CDS             complement(4154..5173)
                     /gene="ruvB"
                     /locus_tag="JD016_10100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAU4999986.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5280..5882)
                     /gene="ruvA"
                     /locus_tag="JD016_10105"
     CDS             complement(5280..5882)
                     /gene="ruvA"
                     /locus_tag="JD016_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAU4999987.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(5875..6384)
                     /gene="ruvC"
                     /locus_tag="JD016_10110"
     CDS             complement(5875..6384)
                     /gene="ruvC"
                     /locus_tag="JD016_10110"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAU4999988.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKAIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6696..7325)
                     /locus_tag="JD016_10115"
     CDS             complement(6696..7325)
                     /locus_tag="JD016_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999989.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7528..7851
                     /locus_tag="JD016_10120"
     CDS             7528..7851
                     /locus_tag="JD016_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HAU4999990.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8119..8973
                     /locus_tag="JD016_10125"
     CDS             8119..8973
                     /locus_tag="JD016_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999991.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9159..10595)
                     /locus_tag="JD016_10130"
     CDS             complement(9159..10595)
                     /locus_tag="JD016_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAU4999992.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(10666..12027)
                     /locus_tag="JD016_10135"
     CDS             complement(10666..12027)
                     /locus_tag="JD016_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HAU4999993.1"
                     /translation="MIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMVRMPINEI
                     STTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNSALAVTTA
                     GFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVIPTPGPLA
                     VAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKNLEQGKDS
                     IKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFIGDKNIAI
                     LLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASGIGNFLVD
                     TMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVLVGLAICA
                     GGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSVINGIIPL
                     PGL"
     gene            complement(12184..13089)
                     /locus_tag="JD016_10140"
     CDS             complement(12184..13089)
                     /locus_tag="JD016_10140"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAU4999994.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLVAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHDN"
     gene            13485..14156
                     /locus_tag="JD016_10145"
     CDS             13485..14156
                     /locus_tag="JD016_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU4999995.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            14268..>14767
                     /locus_tag="JD016_10150"
     CDS             14268..>14767
                     /locus_tag="JD016_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU4999996.1"
                     /translation="MNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEEFGE
                     DKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEELHC
                     PYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCERIY
                     FHDDKVS"
BASE COUNT         4819 a         2609 c         1719 g         5620 t
ORIGIN      
        1 aatgataaaa gaatcacttt gagataccct cttacaaaca actaaatcaa taattttcta
       61 tctttcacac tcttgatttg caactgtaca agaagtttta caagctgatt gacaagaagt
      121 ttgacactct ccacatccac cttttgcagc actttgcttt aaagtagctt gagataaagt
      181 ttttacatgt ttattttcca ttttcaacgc ctccatttct aattgtttcc tattgattat
      241 atcataaaac tactttaata taaactctat ttaaagttga cacccaatac gcctccaatg
      301 cctcctgctg ctgatattaa caataactta taaatcatcc ctacttcaaa tacaaacttt
      361 tcttgtgcta aaaacacagt caaaaaaata catactatgt acaaaagtcc tactaaaagc
      421 ccatacataa gtcctttttc tttcatgtgt tttgaagtgt aaaaccctcc tatagctgca
      481 gaaatagtag ttataaatga tgagaccata gttatattat ctccaccaat atctgtaaat
      541 gtcaaaaata agttatacac taataaaaca gccaaagtta ttatatatgc atatcctaga
      601 cctttaaaaa tataattaga ttttcccata aaaaaacctc ctctcaaatt aatatatatt
      661 cgagaaaagg cttttaaatt actaatttag tcttcttcta tttcttcttt tttagattta
      721 acagttccta tagcccattt ttcaaatgga actttagttc tatttggtcc taattctaat
      781 attacaacat catcttcaac tttagctaca ttagctacta ttccacctat tgtaatgacc
      841 ttgtctccta cacttaggct acttctcatt tcttttaatt gtttgtcttt tttcttttga
      901 ggtctaatca ttaggaaata aaagattgcg attactacta cccatagacc tatactcata
      961 attatttgtt gctgtggcat taatttttca ctcctttgta aattatactc ttttgtaaat
     1021 cataataaat gtttatataa caatttactt tctctatctt tgagtactat tgaatattat
     1081 atattaaaat gattataatt aatagtgcaa aacctatatt tcatatccat attttgcaaa
     1141 aaattcattt ctaaaatcaa gtaatctatc ttccattatc gcttgtctta tttgtttcat
     1201 taaatttaat aaaaagtgaa gattatgagt agttattaat cttgcaccca gtatttcatt
     1261 tgcttttata aggtgtctta tataagctct tgaatagttt ttacaagcat aacaatcaca
     1321 ttcagggtca agtggagtaa agtcttcagc ataagttgca tttctaacta ctactttacc
     1381 ttggctagtc attgcagttc catttctagc gatacgagta ggtaggacac aatcaaacat
     1441 gtctactcct cttataacac cttctactaa gtcatctgga ctaccaactc ccattaggta
     1501 tcttggttta tcctttggca taagtggagt tgtatgctct aacacgtcat acataagtgg
     1561 ttttggttct ccaacactaa gtccaccaat agcatatcct ggtaaatcaa tattagttat
     1621 ttctttcgct gattgttccc ttaagtcttt atacatccca ccttgtatta taccaaataa
     1681 agcttgcttg tcggtattat tatgggcatc tttacatctt tttaaccatc ttgttgttct
     1741 ttctaaagaa ttttttacat attctctatc tgatgggtat ggtgcacact catcaaatgc
     1801 catcattatg tctgaaccca atgcattttg tatttccata gctttttctg gagttagaaa
     1861 atgttttgaa ccatctaagt gtgacctaaa ttctacacct tcttctttta tctttcttaa
     1921 aggccctaga ctaaatacct ggaaccctcc actatctgta agtattggtt tatcccaatt
     1981 cataaactta tgaagaccac cagctctttt tataagttca tgacctggtc tcatatataa
     2041 atggtatgtg ttactaagta ttatttgtga accaatctct tttaattctt ctggtgtcat
     2101 agattttaca gttgcttgag ttccaactgg catgaatatt ggtgtttcaa taatcccatg
     2161 tggagtgtgt aatcttccta accttgcacc actctgctta caagtcttta tcaattcgta
     2221 tcttactgcg tacataaagt tctccttaat catatttatt tctcattatt tcttaagata
     2281 tatttgtaat aagctattca tataatttat tgtctaaata gatttatctt cgtcttcttt
     2341 gttctgttgt tccttaattg cttcttcatc atgaatactc attatattaa gagcactctt
     2401 gtcttttgtt actaaaaact tttttatagc aaatagtcct gctatagcta taactcctat
     2461 taaaacatct atcatacctt catttttagg cagtagcaac atttttctag caattgcgta
     2521 aagtaatgcc tcaataaatg ctcctggtat atgaagagat agcattacta caagttcaac
     2581 acctattact aaaagtaata tttgtgctaa aaaatcatta agctgactgt attgtactgg
     2641 attttgaaaa tcaactatat atgctcccca cataagtctt agaacatcta tagttcctaa
     2701 aaatactgct attagaacta ctactgctaa aacacgttca aatataaaag caatctttaa
     2761 tatagggtta ttcttttttt gtttttgtaa actatgcatc cattacctcc ctactttata
     2821 atcatagcat ctccaaaact gaagaatcta tatctttctt ttactgcctc attatatgca
     2881 tttaaaacat tatcctttcc acaaatagca cttactaaca ttattagtgt agactctggt
     2941 aagtgaaaat tagtaataag tttatctaaa actttaaatt tataacctgg atatataaat
     3001 atttctgtcc atccactagt ttctttcatc tttccatcct cattacaagc tgattctata
     3061 gttctacaac tggtagtacc aacacatatt acattttttc cattttcctt tgctctattt
     3121 attttgtcag ctgcttcttg gctaaccata taatattctg aatgcatctt gtgatttaaa
     3181 acatcctcta cttttactgg tctaaaagtt cctaagccaa catgtagagt tacaaaggct
     3241 atatctacac ctttttcttt tattttattt aacaattctt ctgtaaaatg taatcctgct
     3301 gtaggtgctg ctgctgaacc attatgcttt gaataaacag tctggtatct ttctttttca
     3361 tctagtctag cagtgatata tggaggtagt ggcatatttc caagttcatc caatatttct
     3421 tcaaaaattc catcataatg gaattttata attcttgaac cttcatctga taaatctaca
     3481 acttctccaa ttagctttcc atttccaaaa gaaaatttag tccctacttt tgctcttttt
     3541 cctggtttta ctaaagcctg ccaagtatct tcctctgttc tttttaataa taaaaactct
     3601 attttacctc cagtttcaag cttctctcct attaatctag ctggtataac tctagtatta
     3661 tttaatacta agcaatctcc tggatttaaa tattctatta tatctctaaa tactttatgt
     3721 tcaatattac ctgtctcttt atctaatacc atcaatcttg agctagctct atcttctata
     3781 ggaacttgag ctataagttc ctgtggtaag tcaaatttaa aatcacttgt tttcaatgtt
     3841 aaacttcctc tcttaaaatt tctccagcta caataatatc aaaaaaacaa cttattttcc
     3901 acaatattac cataatctat caagaaattg ctatttttaa cagacaccat atccacaata
     3961 tatagatatt tttccacgat atacatgtct tatccacaat atatagatac aatacacaca
     4021 atttttatcg tgtttatcca cagtatctac agatataatt accattttgt ggatatgttt
     4081 gaatttttag ccattttaga aatattttaa tttaatgact cttttatttt ataaatactc
     4141 gtataatcta tttttatttt tcattaggat atggaatttt taagtgttca tatgctaatg
     4201 gcatggctac tcttcctcta ggacctctat ttataaatcc taattgaagc aaatatggct
     4261 catatacatc ttctattgta tttctatctt ctccgataga agctgccaat gtatctaaac
     4321 ccactggtcc tcctctaaat ttttctatta tagtcataag aagcttctca tctacaaaat
     4381 cgagacccaa actatcgact cctaaaagtt ccagagcatc ttttgccacc ttatcagtta
     4441 tttttccatc agctctaact tgtgcaaaat ctctaactct ttttagtaat ctatttgcta
     4501 tccttggagt ccctcttgaa cgttttgcaa gttctaatgc tccattagac tgtatctctg
     4561 catctaagat gcttgaagac ctaagtacta tttttgaaag ttcatcaact gtataatagt
     4621 ccaatttaca aataacacca aatctatctc taagtggatt tgtaagcata ccagctcttg
     4681 tagtagctcc aataagtgta aatttaggaa ggtcaagtct tatgcttcta gcagatggcc
     4741 ctttccctat tattatatct aggcaaaaat cctccattgc tggatataaa acttcttcaa
     4801 cacttctatt gattctatga atctcatcta taaataaaac atcattttca tttaaattcg
     4861 taagtatggc tgccaaatct ccagctcttt ctattgcagg tcctgacgta attcttaaat
     4921 ttacccccat ctcattggct attatacttg ctagtgtagt ttttccaaga cctggtggtc
     4981 catataaaag cacatggtct aactgctcat ttctcgattt agctgcttct ataaatatac
     5041 taagttgttc tttggatttt tcttgaccca aataatcttc taaagttttt ggtcttaaac
     5101 tgttttctat atctatatct tccatcttca ttgtagaagt tattatccta ttttcatctt
     5161 caaaaccttg catttatacc cctcctctca tacttttgaa aatctacttt atatattttc
     5221 aaatttacat aaactcctta aatattgttt taaaatccaa atattataaa atccaaatac
     5281 tatttcaagg atttactcat tatatatgta agtgctttct ttataatagc ttctgtattc
     5341 ataccatctt ttttacattt ttgaactgcc tcttttgatt ccgacaaagt atagcctaaa
     5401 gctacaagtg catctacaga ctcatcttgt gatatcaagg taggtttttg tgataataac
     5461 gttggttcaa actctatgtt atttttgtct accttatcct taagctctaa gacgattctt
     5521 tctgctgtct ttttaccaac accaggagct ttggataatt ttccaatgtc ttcacttaaa
     5581 atatatgctc ctaactgtgc tggtgatgca aatgatagta ttgaaagcgc cactttaggt
     5641 cctattttag atacagatgt tagtaattca aacatcttaa gttcttccct acttacaaaa
     5701 ccacaaagac tcatatcatc ttctcttaca attaatttag tatatatttt tgtggcttct
     5761 cccacttgta aattcattat tgtattggat gatacattta ttttatatcc tatcccatta
     5821 ttttcaacaa ctatactgtc tatataaatt tcttctactg taccttttat ataactatac
     5881 atacttacca cctatattct ttagagtttt ttcaagtttg tttgaatgag catggcaaat
     5941 agccacagct aaagcatctg caacatcatc tggttttgga acttttttta gacttaaaaa
     6001 tgaagttacc atctgttgaa cttgtgcctt atctgctctc ccatatccaa ctacaccttg
     6061 ttttacttga agtggagtgt attcatatac aggcttccca ttatgagcac atgcaagcat
     6121 agtaacacct ctagcttgtg caactgttat agctgttttt acattcttgt taaagaataa
     6181 ttcttccatt ccaacttcat ctatattgta attctttact attgtatcaa ttcctttata
     6241 cacaagttcc aatcttctcg atatattcat atgggcaggt gttgtaactg ctccataatc
     6301 gattgcttta aacttgctat ttttgtattc aattatacca tatccaacta tggctatacc
     6361 tgggtcaatc cccaatatta tcatttttta aaattccccc taaaaataaa agaaatacca
     6421 aaatgtattt ctcataaaaa cttatgttct aaatcattat atcataaata atattaacaa
     6481 atcataaaat gctctatgta aaatataaaa tcaaataaaa aagttatatc aaagcaagat
     6541 tttacctcaa tataactttt atatttatta caaataaatt attgtatttt tataggaatt
     6601 aaatgtgact acaattttaa tactaatcta tttaatatta aattatttat tattaattta
     6661 cttagtattt aacttaaaat aatattaatt tactattaac ttgcaaagtc ttctaatctt
     6721 gaataggcct cttcttcagt atccataact acaccttttt tgatttcatc ttgaactgtt
     6781 ttaggaagtg aatctactct tatttttgtt ttttctacta atgttttact tccgctatca
     6841 tcatatttat atagtgctat atttccatta tcattttcaa tagtatattt accttttata
     6901 gtaacatcca ttgcttgtac atctttatta tcactatatt tagattctac caatatgatt
     6961 tcatttttat ccatactctt tattttttta gttggatatt cttttttaaa atatgatact
     7021 atttctgact tcgtcttatc taataatgac tctggaacta ttcctgccat tgtaagtgtt
     7081 ctcttatagt tagtttcatt ttcgccttca ctttgaagcc aaacttcaca atttttattt
     7141 aatccaaaag cttcttttga cttttcattc atattcgcac tagtacttac aacttcacca
     7201 gtttgaactc cattgtgttt taatcttgtc cctatataaa ctccgctact taatacaaat
     7261 actccaacca gtattaaggc agatattttc caaggaaata attctttttt cttcttttca
     7321 aacattgatt acgcctcctt tttctattat tttctccaag ctgtaagttt ttatacaatt
     7381 taggtaaatt ttagaagaaa atatatttat atttcaaatt actctaagaa tagatatttt
     7441 ctaaaaggta gttttctctt aatggttata ttcttgtaat tatatggaca taatttataa
     7501 taaataatcg tttaggaggt aataactttg gaaagaataa actgtaatgt aattaactgt
     7561 tcacataaca acagccatat atgttatgca gataaaattt ttataaatgg tgaaaaagct
     7621 cacaatgaca gacatacttg ttgtagttca tttttagatg aatctattta tagtaattta
     7681 tctagcaacg ttaataatag aaattgtgag gaattagttt gtactgtaaa aaattgtaaa
     7741 tacaacaaag atggtagctt atgtacactt gataatatat tggttgctcc tgaaactgat
     7801 agagtaaatc tttatattga aacatgttgc tctagtttcg aatgtaaata gtcatagtat
     7861 aaatataagg gtgtcaaatg taattaaact aacatagtgt agagtatcta caactattag
     7921 tttgattata cttgacaccc ttatttattt taaataaaat ttcattatta taaccatgtt
     7981 tataatgttc taactgtaat taaatttgat aaatttctat aaattggtat aatcaatatg
     8041 taaattgaat ttagtaaatt aatcataaaa ataaaatatc gagtatatag tgttatacta
     8101 actagaaatg aggtgcttat gagtacattt tatattcaat ttctttattt tgtaatttat
     8161 agctttatag gatggtgttg tgaaactaca tattgttcag ttttacaaaa acaatttgta
     8221 aatagaggtt ttttaaatgg tccattttgc cctatatatg gatttggagc attagcaata
     8281 gttgctacct tgactccttt tgtacacaat attcctttgt tatttttgtt tagtataatt
     8341 ataactagca taatggagta ctgtacgagc tttattttag aaaaaatatt taatatgact
     8401 tggtgggatt attctaaaca taaatttaat attcatggaa gagtttgtct ggaaaactca
     8461 cttatgtttg gtgtattatc attgattgta atgcttatag tacatcctat tgtggttgac
     8521 tttattaact ctatctctaa aaatgtgcta tttatatttg ctatatctat agaaatttat
     8581 tttgttttag atttagttat cacagtccat acaattttac aattaaatgg taagttaaaa
     8641 caaatcaatc ttataattaa ggaattaaaa gataagaaag aatactacaa acttattaca
     8701 caagaaacta tagaaaacaa acttgaatct ttagttgaaa atagactgga tactttggaa
     8761 gataggtttg aaatacttaa agaaaaagaa aactatcgct atgctaaaaa tcgtattgat
     8821 gagctaaaaa ataaacttaa tactttgctg tctaaccaca aattacttca tagacgtata
     8881 atcaaagcat tccccaacat aagctctaat aagcatacag atattttaaa taaaatcaaa
     8941 gaaaatatta aatcaattaa aaaagataaa taataaaata aacacataag ttaaataata
     9001 aaaatactaa tacataagat taaggtttag aatatttttt gtaaaatact ctaaacccta
     9061 attaatatac tattattttt aatcttaatt tataatttta gtttatttta attttaactc
     9121 agtaaattta agcgtttaaa cttcaataca ctaatatact accccatcaa tatctcaact
     9181 gattctctaa gtgtgctaat ctctcccaca tttccaggga aaattatata tggcatatta
     9241 ggaaatttgc tttctttgcc tgtcatccat actggtatac caggttttat ctgtcccatt
     9301 actgtagctt tttttactct tagtgcttta gtaccaacat cactagaagt aattccaccc
     9361 ttagctataa taaaatttgg tcttacatta agcataccaa ttatactggt aactgcatct
     9421 gatatttcta cagaaatcat aagctgttta tctttatcat tcgtatccaa gtcaaaacgc
     9481 tctcgtctag tataaacagc aacagtttta cccctgctta ttttttcctc tacaactttt
     9541 ataactcttt ttacctcatt ttttagacca ttttcttgaa gaactaaatg ttgattaaat
     9601 tctataaatt ctatcggata tttacaattt ttaagttctt ctaactgttg ggtagtctta
     9661 ttcacatgtg aaccaacaag tataattcct ccattggtat taccttttga tactaattca
     9721 tccttagtaa gtagttcctt gtcactcact ccgccaagta cttttgttat ggcagcagcg
     9781 cttctaaaca taaattcctt accactattt actgctcgta taaacgcaat tgtaaatacc
     9841 tttacatcta cataatcggt tgcatttaca ataattttat tgaatccttc agctttttta
     9901 agtttttctg ttatagcttc aatatttagc tctctaagtt cttttgacga aatatatatc
     9961 atatcactag atttgtactt tccatttgtc ttttcttcac accactcacc tatatcagat
    10021 gatttatatc caaaagattt atcttttgca aattcagtca ttcctgctgg tacaagtatt
    10081 tctccttctt taacataatg tacattatct atagtaaacc taccaccttc tttaaaaaat
    10141 ggcattatta tttcaccctg aaataacttc cccgataatc tctctacttc gctttttaat
    10201 aaattagttt caagaggata atgtcctctc atagtagaat cactacgact tatgattata
    10261 aaatccttat taactttctt tgatacatcc acaatattct ttgaaatttc tttatgtgct
    10321 tttgtagttt gagcaacagt aaatcccctt gaattggtaa gtataaaaaa catactattt
    10381 ttctcattaa aaccttgttc catactatcc ttatcccaat cagtataaac agaaatatca
    10441 tgaactgttt gtacacctgt tgggtcatca tctaatacaa caatttttct atctaaatgc
    10501 ttaagctcat ctgcaagaag tttatttaat ctgtcctcat ctacttgttt aaaagtatta
    10561 agtatatcta tgctaattgg ttttacttct ctcatttaaa acgccccttt atgtaatttg
    10621 tatagtctaa atgtaattta tgtgatataa acaatagttt gttatttata gacctggaag
    10681 tggtataatt ccatttataa cactaagcaa taaaaccatt ataaatgcta caactccagc
    10741 aatggttcct ccaattgtcc aagagtttaa agtatcttta actgataatc cactaaatct
    10801 tgaaagtacc cagaatccag aatcatttgg aagggagaaa ccaactcctc cagcacatat
    10861 agcaagccct actaaaactg gtgacattcc agttgccaaa attgtaggtc ctaaaattga
    10921 tgatgttgtt actaaggcta cagtagcaga accttgagaa attctaagca aggcacttaa
    10981 taagaatcca agcactacaa ctgatatact aagaccagac atagtatcaa ctaagaagtt
    11041 acctatacca cttgcattta taacactgcc aaacgcacca cctgaacctg ttatcaataa
    11101 tattagccct gctgaatcag cagcttcaat aactacatct cccataggtt tatttatgta
    11161 tttctttaaa aatcctattg ccaccaatat tccaagaagt attgctatgt ttttatctcc
    11221 tataaatgca agtactccac tcattgtaga acctttttct aagaacatac taaatacatt
    11281 tgatataagt ataagaagta ttgggaacaa taaagaaaac atagaaattc caaaacctgg
    11341 tactttagaa gaatcatcat cttcaatttt tatgctatct tttccctgct ctaaattttt
    11401 ttctctatca ttctcatcaa tagcataagc tggatatttt tcaaaacgtt ttccataaat
    11461 ccatccacct ataagagctg ctggaaaagc aacaattata gaataaagta taaaactacc
    11521 tacatttgca tttacatttg ctgcaactgc taaaggtcct ggtgtaggta ttacaagtgc
    11581 atgacctact atagtcccaa ctcctaaggc acatacaaaa accattagag atttcttagt
    11641 tactctcgaa acatacttaa ttataggcat cattataaca aaagctgcat ccataaaaac
    11701 tggaattgaa attaaaaaac ctgctgtagt tactgctaaa gcactgtttt tttctccagt
    11761 cttagctaac aacccctttg caatagattc tgttgctctt gattctgaaa gaatatttcc
    11821 aaatattatt ccaagtccaa ttacaatacc tatactacct aatgtatttc caaatccagt
    11881 cgatattgtg gttgatattt catttatagg cattctaacc ataaagcctg taattataga
    11941 tgttatcagt aatgctaaaa acggatttac tttaaattta ataatcgata caagtaaaat
    12001 tcctatagat ataacaaatg ctatcaaaag tccatatcct gtaatcataa tctttacccc
    12061 tttatcattc tactttatta tttatcaatt ttatttttct tttactatga taacttaacc
    12121 tcgctgtatt tatataatct aatcctaata atatgtattt aattatattg atatatttaa
    12181 atattaatta tcgtgttttt ttatttcaac acctgccaac ttttcaaaat actgtgctat
    12241 tccactatgg tcatcttcca aatgtccatg tacttttaat gtctgcataa tttctaataa
    12301 ttgacttgtc ataggtagcg gtacatctaa attgtgtgct gtttgcatta cattcttaat
    12361 atctttcaaa ttaatagata tttttccacc tggtttaaaa tcacgattca taatcattgg
    12421 tatcttcgca tccaatattg tactaccagc taggcctcct cttattgcct tatatacttt
    12481 ttctggatct gcacctgctt ttgctacaag tacaaatgcc tcagaaactg ctgctatcgt
    12541 caaattaact attacttggt ttgtaagttt tgtaacactt ccactaccat tatctccaat
    12601 taaaatagat gagcttccca ttatattaaa aaatggctct actttcttat aaatttcttc
    12661 cttaccacca gccataaaag caagtgtacc atcaatcgct tttggctccc ctccacttac
    12721 tggtgagtct ataaattcta agcccatatc ttttaattta tttgcacaca atatggattc
    12781 tgtaggagta actgaactca tatcgactac tatacttcct tcttttaaag tttttgccaa
    12841 tccatccatc ccaaacaata tatcctgcac aatagttcca tttggaagga ttgtaaatac
    12901 aatatcacat tcttgaccca tttctaaaac acttgcgtat tttgctccac atgatataag
    12961 ttcatccaca gcagatttat taatgtcata caccaaaaga ttacatccat cttttaataa
    13021 attttttgcc attggctttc ccattattcc taatccaata aatcccaatt tcattttata
    13081 accctccata tcttcacatt tttgaaaacg ttttttatgt tttagagtat attacatacc
    13141 agtataccgg tcaatataaa atataaaagt atttatttta acaataaata gtaattttta
    13201 aagtcattta ttatatttag tcatcctata taagttttaa tacatactat tgttttcaaa
    13261 tataatatac tacttttaca gttttgtatt ttaactaatt tcaattagtt ttaattaatc
    13321 tgcctttaat gttaatcatg cttcacactt caataatttt ttatattttt taatcttatc
    13381 tgcaatttaa attgcaaaag attgacctta ttttcactat gttgtatgct taaaattata
    13441 taagtttttg tatttttata ttgtttaggg gggtattatt aagtatggct acatttaaaa
    13501 gattacaaga acaagcatat gactacttaa aagaattgat actatctgga gaaatgatag
    13561 aaaatgaaat atattctgaa acaaaacttg ctagtgaaat aggtatctct agaacaccta
    13621 taagagatgc tcttcaaaga cttagtcaag atggatttat agatataatc ccaagcaaag
    13681 gatttagaat ccatcaaatt acagcaaatg aaattgtaga gatattccaa atacgctctg
    13741 caatagaagg attttgtact tttttaatta caagtcaata caaagaagct agagcagtag
    13801 aaactataag taaattaaaa catcttttag ataaacaaaa agatatttta ttaggtgata
    13861 aaaatttaaa ctcatttgca gaatacgata cattatttca cacaactata gtttcatatg
    13921 ctcaaaatac tgaatttgac aaaatgttta acaattatat gtacagaata aaaaaattag
    13981 ctctcgattc attgtcacat gaaggaaggc ttgaaacaac attaaaagag cacacagata
    14041 tttttaataa tattgcaaat ggatgtattg aagatattta cagaactact ctcattcata
    14101 tggaaactcc taaatacatt aatttagaag atttttgtaa caataagtta ttttagtatt
    14161 atactttcat ttattaaaaa tatatctatc tcaaattaat ttcctaattt ataaaaaata
    14221 ttctataatt atattgtaac taatacttat tctaaggagt gatttttttg aacgaagatg
    14281 ttgtaactaa tgaaactatt gacaaagatt atgctattga agaagttcgt atggcatgca
    14341 aacattttgg tgatttatat ttttattttt ccaaagtgtt atttgaagag tttggtgaag
    14401 ataaaactag cgaaatatta agaaaagttt tatttgaaag gtctgaagaa agagctattg
    14461 ctatgagaga aagagctcat gaaaatggag atgaattaat tgcagacaat attattagca
    14521 caactgatgt tccatttcta ggatgggttc ctgaatttga agagttacat tgcccctatg
    14581 gagcttcctg gcttttaaga tttgaggaaa atccttggtt taaaaaattc gcttctcttt
    14641 actgtgatgt tacagatact actgtagctg aagtatttac aggagatact tcccataaaa
    14701 taacaaaaaa cattctatgg ggagataaaa gttgtgaaag aatatatttc catgatgaca
    14761 aggtatc
//
LOCUS       DACXMZ010000085        14348 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.085, whole genome shotgun sequence.
ACCESSION   DACXMZ010000085 DACXMZ010000000
VERSION     DACXMZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.085"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            74..895
                     /locus_tag="JD016_10155"
     CDS             74..895
                     /locus_tag="JD016_10155"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HAU4999997.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(971..2359)
                     /locus_tag="JD016_10160"
     CDS             complement(971..2359)
                     /locus_tag="JD016_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU4999998.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGSLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVCNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(2494..3252)
                     /locus_tag="JD016_10165"
     CDS             complement(2494..3252)
                     /locus_tag="JD016_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HAU4999999.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGVGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(3254..3970)
                     /locus_tag="JD016_10170"
     CDS             complement(3254..3970)
                     /locus_tag="JD016_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HAU5000000.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLICSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(3972..4679)
                     /locus_tag="JD016_10175"
     CDS             complement(3972..4679)
                     /locus_tag="JD016_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HAU5000001.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            complement(4795..5331)
                     /locus_tag="JD016_10180"
     CDS             complement(4795..5331)
                     /locus_tag="JD016_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAU5000002.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(5433..6503)
                     /locus_tag="JD016_10185"
     CDS             complement(5433..6503)
                     /locus_tag="JD016_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HAU5000003.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(6496..7554)
                     /locus_tag="JD016_10190"
     CDS             complement(6496..7554)
                     /locus_tag="JD016_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAU5000004.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(7541..7873)
                     /locus_tag="JD016_10195"
     CDS             complement(7541..7873)
                     /locus_tag="JD016_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAU5000005.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            8072..9193
                     /locus_tag="JD016_10200"
     CDS             8072..9193
                     /locus_tag="JD016_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HAU5000006.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(9321..11219)
                     /locus_tag="JD016_10205"
     CDS             complement(9321..11219)
                     /locus_tag="JD016_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAU5000007.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            11534..12310
                     /locus_tag="JD016_10210"
     CDS             11534..12310
                     /locus_tag="JD016_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087838.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HAU5000008.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYIKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(12402..13154)
                     /gene="xth"
                     /locus_tag="JD016_10215"
     CDS             complement(12402..13154)
                     /gene="xth"
                     /locus_tag="JD016_10215"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAU5000009.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(13315..14265)
                     /gene="galT"
                     /locus_tag="JD016_10220"
     CDS             complement(13315..14265)
                     /gene="galT"
                     /locus_tag="JD016_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAU5000010.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
BASE COUNT         4858 a         2360 c         1556 g         5574 t
ORIGIN      
        1 ttttatagta atatatatgt atataaatat tttggtatca ttataattaa tacatactaa
       61 tggagggttt tttatgcaaa aaaaagtcgc agcaattaat gacttatctg gaataggaaa
      121 atgctcatta tcggttgcta tacctatact ttcagcatta aaggttcaat gttgtccatt
      181 tccaacggcc atactttcaa gtcaaactgg ttatcctgaa tttactttct tagactttac
      241 tgatgaaatg gtaaaatatt caaatgtttg gaaaaattta aaagtcaatt ttgatagtat
      301 atatagtggt tttttgggtt caaagcatca aattgagata gtggctaatt ttataaacga
      361 ttatcccaat gcttttatcg tagttgaccc tgtgatgggt gataatggag ttatgtatcc
      421 aatattcaca gaagaaatga gacaggaaat taaagaatta gttaaacatt cagatttaac
      481 tactcctaac ttaacagagg cttgtttctt gactggaaat gattatacta aaagcgatta
      541 taatagagat gagcttatat atattgcaaa atctgtatct gatttgggac ctagcaaagt
      601 cgtgattact ggtatattag aagatgataa tatattaaat ttggcttacg atagagataa
      661 tgaccatgta ttctttacat cagtcaaata taataattgt tcttacagtg gaactggaga
      721 tatattcaca tctatacttt gtggtatgtt agtaaataaa catgatttgg gagtagcggt
      781 taatactgca acagatttta tatataaaac tataaattac acatctcaat ttgatactga
      841 tagaaatgat ggagttatgt ttgaaaactt tttatcagat ttaactaata tataaagtca
      901 aaaaaactat ccattttata aatacctata aatcataaaa tttaggtatt tacaaaatgg
      961 atattattta ctaaactatt ggtatttcaa ttgttaccat agcaccacca gttttcacag
     1021 aattttcaat atataaaatc ccattatgtt tttgtacaat attatttaca atatagagtc
     1081 ccattccata atgagattta gaatttctac tcaaatctcc tgtataaaac tgttctgtag
     1141 cttttgacaa atctgcttta gaaaaaccac ttccacaatc tgtaactaca aattttaagt
     1201 atttattaca tactgataca cttatgtata gtttactctg gcttggtgaa taatctatag
     1261 catttgaaat tatattcata atagctctga ataacagttc tctatctata gttatatttt
     1321 taggcaaatt atcttgttta taaaactcca catctaagtt tttagtacta gctaatgctt
     1381 ctatttgcac taagacatct tttataaact cactcatact gattgcttct ttatttaata
     1441 tatagccatt ctctgttttt gatatatcta ttaaagtatt aatatatttt tctatttgag
     1501 tgacaccctt tgagatatat tccatgtatt ctaataactc aggattttca tttatttcaa
     1561 ttaataagtc agtatttcca tgtataattg ttagtggtgt ttttatatca tgtgccagtg
     1621 cagatatctg ttcctttcta ttttcttcta gcatccattg tttttctaaa gaatctttta
     1681 acgccaactt cattttatcc atagaaaaaa ggacattatt aatttcataa attttactag
     1741 attctacaac aaaatctaaa tcctgttgtt ctattttttc tgtagtattt tttaaaagct
     1801 ccatttcaat attaaatttc ttgccaaata cttttgataa tataacaatt tcaataaaaa
     1861 atattatact aaaaataatc atacccaatg tctctggttt tggtaaatat gctcttaaaa
     1921 ttggagaact atactctgat actattttgt attttattat aaaaacttca tctttcttaa
     1981 aaaactttat ataactttct gaatatgcat gcctttcttc aatatctctc attaaattcc
     2041 atacttcttt tgattctttt ttattaaagg tacctgaaat taaactccca tccaaagtat
     2101 aaaccccata ctcatatata cttggaatta tatcttcagt tactttttca cttgaaataa
     2161 tctgttcttt atatttatta aattgttttt ctgcataatt agctggtaaa attacacctg
     2221 atgataataa tacagaaaat gataacatta aaaggagtac aattgaaatt gtcataataa
     2281 agaataaaga taaatatttt ataaaaatag ttcttaactt ttcaactttt ttgttgttag
     2341 attttttacc tccaagcatt tgtaccctgt cctccaatag tttaattaat tcataattaa
     2401 tacctagcca gttttttatt tgattatttt atatcttttt attgctaagt tatattttaa
     2461 cataataata taaataaaca gtcaattttt actctattct tctgattttt taccttccca
     2521 tttagaaaac caaatacata aaattacaaa tgctaaaatt gttacaaaac cacacattaa
     2581 acctgttgat tgatatgctt ttattgattc aacctttata tattcaaccg ttttagaact
     2641 tgaaaaagac gaccaaatag ttataaatct aacaccccat gcacaaggaa aatactgcca
     2701 aataccagaa cccaatccag ttaataataa tgctgaaagt aacatttcaa ctatacctac
     2761 tccaattgat gttccttttc cgaaccttaa acttaagaat aaatgtaata tgtattcaaa
     2821 taaattacac ccaattaaaa ttagaattga aatcatataa aagcttgatg taaagtaagc
     2881 cttagatata ccaaatttaa aacctaatac agacactaat gttgctacaa aaccaagaca
     2941 tactaagatt atatatttac ttataagagt aagatatttt ggattagatg ttgttaacat
     3001 atgtttataa ttccctgcat aatattcttg ttcaacaaac attgaacaca caacacttga
     3061 caataaagga aaagctattg ctataacttg taaatatgct actatcttta gtatattatc
     3121 agctcctgaa accttataat accataaaaa caatcccacc cccattaaag caatgcaaat
     3181 gtgcattagt attataaaat ttctctttaa ttttattaaa tcagaacaaa atattcttat
     3241 taactgtctc atattacttc acctctctat tattgaacca aacccttgta gctaaagcaa
     3301 ataatacaaa taaaacaata gatattaata ctcctggcaa aataacagca ttatttaaaa
     3361 attcacttcc ctctggtaca ggaagaccgt ttggcagaat atgcaataca acagccatta
     3421 atcttgaagg aatcgaatat ggaaacatat accacatttc tgtagttgag aaaacagcaa
     3481 ctccaacaat agtaaagaac atatttatta gaactgtaac aaaaatacca aattttgaag
     3541 ctaaaaacat acaaagagga atctgccaca taaatagtaa aataattaat atagttgcaa
     3601 tgatattttg acctattgaa atagtttctt tatagattat cccaacaaaa actacaccta
     3661 ttgcagaaat aattgatgct ataaaacaac aaataataca gtaaatcatt tttccagtcc
     3721 aaacatcttt catttcaatt ggcaatggta aaataccacg atatttactt ttttcatctt
     3781 ttaatactat taaagaacat attaatgtta atgctcctgg aagaaatgtc gtataccacc
     3841 agttgaaagc agcattttgc cctccaccta gtccacacaa aagtatacaa aacactggaa
     3901 ctatccatat caacttcatt ccaaaagttc ctttcatctt ttgaaattcc actctaataa
     3961 atctttccat attactgccc tctctttcta ttttcagcta caactttcat aaaaagttct
     4021 tctaagtttt gttcttttga tacttctcct tgataaccca ttactccatt agaaattatt
     4081 cctatttcat ctacaacttg ttcaacttca gacaagatat ggcttgataa aataactgta
     4141 attccttgtt ttggaaaaga acgaatcaat tctctaagtt cttcaatacc aatagggtca
     4201 agaccatttg taggctcatc taagattaaa agttttgggt gatttaataa tgcaatagca
     4261 ataccaaggc gttgcttcat gcctagagag aattgacctg aacgcttttt aaaggtatct
     4321 cttaaatcta cagtctctaa aacttcttca atgcgagagt caggaagacc aagtaaagta
     4381 gtatggactt ttaaattttc tcgtgctgtt aaattctcat atatagcagg agactcaata
     4441 agagaaccaa tatttgataa atcctttcta gtccatctat gtccctcaaa cattattttt
     4501 cctgaagttg gatttaaaat cccagttatc atttttaata aagtagattt cccagctcca
     4561 tttggtccta ataatccata tatagaatta gatttaattg ctagtgaaat gttatcaact
     4621 gccatctgct ccttaaattt tttagaaact ccttttacct gcaaaatata attactcata
     4681 attttcatcc tttcttttgt caaacttatt tacaagaatc attctaaatc ataaatataa
     4741 ggattttata agaattaaaa aacaaaaaaa gatgtgttaa aaacacatct ttttttatct
     4801 taattctatt aattttccat tgtagtatat ctctacttct tcaatccaaa tgctattatc
     4861 ttcttcttgg atgacatttt taataatatt atttatagta tatattatat tttgttcatt
     4921 caaataatca tctttccaag aaactctact ttttttatct ggttctatca agtttatttt
     4981 aacaagatta tctttttcct taacttctac ttccatgtct aaaccattaa aatactcatt
     5041 tgataatgta tctaagatta atcgcagttt atccttaagt gaactttctt catcaatctg
     5101 taagtacttt actattttct catcctttga aattatctct ataggtattt tagtagtttt
     5161 tttagaatta atcattttac tagttgtttc ttcttttgca tcttcatcct tctttacaac
     5221 tgctgattct ttagaatcaa ctagctcaac tgcactgatg ctataatcca tacatgccgt
     5281 actcaataaa gtagtagcaa ttattaaaaa acttactgct attttattca taagtatccc
     5341 tctcctaatt ctctttcgat acttattata ccatagtata gattatattt ttatactttt
     5401 aaatcaattt ataaattatt atagtaaatt atttacaatt ctaagctatt tgaatttatt
     5461 tttatagtat attctttctc taaattcgac agatttttat tcaaaagccc ttttatttcc
     5521 ctcgtatttc tatacttgtt gctatgttct tcattatgtt cataatcata tgaatcattg
     5581 tcatattctt catcattatt aaattcttca attgcttctt ttgactttat atcacaatta
     5641 aatttatact tatttactgg tatatcaagt cttaccttag aatttggaac ttctatgtcc
     5701 aggaacttag caattggcat atcagagtac aagtaaaatt cactttcata atttgaattt
     5761 ctacctattt tctcttttat atttacattt tcaggtatat actcttctat attattgata
     5821 aatacatctt cattctctga agatatatac aatgactctg attctatgtt tacattttta
     5881 acgcctaaaa ttgattttgt tgacaagtga agattgttat tagatattaa gtttaggttt
     5941 tctaatctat ttactttgtt ctctgttata gctctactaa aacctccata agaagtttta
     6001 tatgttatag tatttgaagc tacattatcg tatacaaata agtgtgagct tatacttgat
     6061 gcatttatgt ctacattatt tggtacatat attataattt ctctataatc atgtgaaaac
     6121 atactattta tagatgtatt taataattgg tcgaaatttt taatcttttt aattttattt
     6181 tcatattttc tttcaccttt tacaactaaa gtcttatctt ttaaagttac attgtatttg
     6241 taaaactcat tatttcctct tgttgttaca cgcacatctt cacttgaact tttctttatt
     6301 gtaacgttta tattgtcaac agctatatct aacttgtcaa tatttacttt ttcttgataa
     6361 agtttatttt cttttttaaa ttcagcttcc ttttctaaac caaagtttat taggcttggc
     6421 ataactaaaa tccctgacca tgttgtccct attataccta ttaccaataa aactgcagca
     6481 aatatagcaa gttttttatt catgtttatc ttctcctttc ttattttcta tatcttcaag
     6541 gtccttatat atcgcctcaa aatttatact atcatcatta tattcatcag tttcttttat
     6601 gctttcttct tctctattgt agtaatcatt attatatgaa ttttcattaa tgtctttatt
     6661 caaatcttca tcatttaaag tagctttttt tcttttttcc atcttttcat ttgcctttat
     6721 ataaatattt tttctcttta tccaatgtat ataattattt agtaattgtt tacctagttt
     6781 tatggcaaat aataaaattt gccatgcaag tatctcaagc cctacaaatg ctatggttgc
     6841 gaaaatacaa aacatcataa catctggaac cattgctata aatttaatca caaatggaat
     6901 taatataaca aaagcaatta atgagcatac taatgcaata gtagcagata caatagcaca
     6961 tacaaacgca aatgctggaa caattagaaa taaagataac actactaata acccctttat
     7021 aacttgtctg gataatcctc ctacaccttc ttctatactt ggtgtaagtt tatcatttat
     7081 attttctttt gatttattgt acttttcctt agcttcatct ttcaagatag agtaatcctt
     7141 ttttacattt gatttaaatc tactaaattt accatttaat atgtctgtat tcattatttt
     7201 tccatctata ttttcttttg atttactata cttttctttt gcttcatctt ttaagataga
     7261 gtaacctttt ttcatatctg atttaaattt actaaactta tcattcaaaa catctgtatt
     7321 tattattttt tcctcttcaa tatttttttc tttagtttgt aagataagtt cttttgctat
     7381 agattttggt gagccaagtg atgctattat ctccatatca ctctttccct ctataagacc
     7441 atctacgaaa tactcttcat aatctctcaa tatatcatta acttcgctct cagaaaaatc
     7501 tttttttaga taatccttta aaatttcaag aaactcagtt ttattcaagt tcattacctc
     7561 cctcatcttc acttattaaa atctcaacag cctttacaaa ttctctccac tcttccatca
     7621 gattggataa attctctttt cctagtgaag ttattctata gtattttcta gcaggacctt
     7681 cattagattc tagtatatat gtttcgaaat acaaatcttt tgtaagtctt cttagaatag
     7741 gatatattgt tccttcattt acatctatta ctttagagat gttatgtact atctcatatc
     7801 catacatatc tttcttagat atgagagcaa gtacacaaat ttctaaaaca ccctttctaa
     7861 attgtgtgtt catatactcc atcctttctt taaatacaca gtattgtgtt ttacaaggta
     7921 ctatgttaaa acttcttata tcctcataat aacacagtac tatgttctgc acaatagtta
     7981 tttttaaata ttcttgttga ttaaatcctt ctataaatgt ataatactct aatcagatat
     8041 attttatgaa agtggtgata aattggctaa aatgaaagat gataaaacaa tattaatcct
     8101 aactgctcaa tttggtgctg gacatataag tgctgcaaaa gcagtcaaag aatgtattat
     8161 tgaaaaatat agtaactata atgttgttat acaaaacttt ataaatgcaa gtattcccat
     8221 gatgaataag cctatggtaa aactttatga aaacaataca aaatatactc ctggattata
     8281 taattactat tattacttta agaaatcgtt tgattcaaga catgatttat ctcataaatt
     8341 atatacacct aaactttctg agtatattgc tgatataaat ccagatttga ttatctctac
     8401 atttccacta gctgctgctt gtgtaaataa ctttaagata aaaaatcctg atataaatat
     8461 acctacactt acagttataa cagatgttgt agatagtatg gaatgggttt ttgaaaatac
     8521 agatttgtat tttgttcctt ctcctgaaat aaaaaataga ttttttcaga aaggaataaa
     8581 tccagactcc ataaaggtta ctggagttcc tgttgataag agatttcaaa ttgaaagtaa
     8641 agaactatgt tgtgataaat atagactatt acttctaggt ggaggaagag gtctatttga
     8701 tatagatgag gattttatgc attggataga tgaatttatt gaagaacata gtaattctat
     8761 agaaattaca atagttactg gtaaaaacaa aaaattatat gacaatctaa ctcataaaaa
     8821 acctctaaaa aatattaaag tacttggatt tgtaaacgac atgtataatc taataagaga
     8881 atgtgaccta atgctaacta agcctggagg agcaactata tttgaagcta ttcaatcaca
     8941 aactccagta ctcgtaaaaa tgccaaaagt tggacaagaa attgaaaacg ctaaatttat
     9001 aatagataag ggtcttggca tgatttatag tgatgaccta gaccttaaaa acatatttta
     9061 taaattagta tcaaatgaat ttgattctat aataaacttt atgaaaaaaa atttagaaga
     9121 atttaaaaca gttattcatc cagagaaaat tgctgattat atatcagaat taatagataa
     9181 tcattacagt taaattgtac tattaaaaaa tttcaaacat aaaattaaag gcaatcaagt
     9241 atgcttttac acttgagaat ttactcatag tgtttaataa tacttgattg cctttatcat
     9301 atcaaaatca aattgttata ttagtatata tttttcttgt actgcatata cagttgttgt
     9361 atttcttcca tacttctctt taattctatt tgtaaatctg aagcagtctg gtaatcctta
     9421 ttttctatag cttctctcac tctagtgaat agtgattctc tagttaaagt atccttcata
     9481 atattgaact ttaaatccga tattgagttc caaactacct catcaagcgc atccatattt
     9541 tcttttgtgt gtatcttgac aatacttctt attttttgaa tcccagcctc tattattcta
     9601 gtttttaatt tcttttgcca cttatctata gctccaatct taaatgattc tattatacta
     9661 tcagtaaata catcaccttg ttttaaagat tttaattttg aagcatatat ttctaagttt
     9721 ttcatatttt cataaactgt agctggtggt attccaaatc ttgcatttct ttcttccaaa
     9781 ctataatgtt caaatacatc attttcatct ctatatgctc tatctttttc taaatagaaa
     9841 ctttcttcac ctacattttt agataactct ttttcaagtt cttttgtact aaggccactc
     9901 ttagctgctg cttttatacc atccaacatt gtttgataac atcctgctat tactaaatac
     9961 gtgttagata gagggcttgg tgatcttaac tcaaatctta cagtctttgg atttttcata
    10021 tctcttatta atccaacaag cactgaccta tttcttgatg gaatctcata actatgccct
    10081 aaagatgtaa ctatgcatac aggagcttca aatccaggaa ctaatctatt aaagccatca
    10141 ttagaagctg ttacaattgg gtttaatact tcataattgt aaagaagtcc cataagagcc
    10201 ccatatccta gttcacttag ataatcttct tttaaatctt ttggagcaaa tagatttttt
    10261 atacttccat cctttaactt cgcacttact cctacatgtg tatgtccacc acttcctgct
    10321 actccatgaa ttggttttgc tttaaatgta acttctagtc catgtgatgt aaatacatcc
    10381 tctataacat ctcttactaa taattcattg tctgctgctt gtaatggtgt agaaaacttc
    10441 caagaaacct ctaattgctc catagcatgg tttgttcttc catctatact aatagaactt
    10501 tgtattcctc cgacctcttt gtgagccatt tcagggttta cacctagctt ttgaagtata
    10561 attaaacttt tctctaaaca agttcttata ataccatgag ttcttttcca gtattgctct
    10621 tttaggcttt gtgatacata taatttctct aagtcagctt tatcctcagg agtattaacc
    10681 cagaactcaa gttctgtagc tgctgttaac attatttcct ctatatcatc tactgaatct
    10741 attcctatgt tatttattac atgtggatat tctctaaata tttcatacat tgacttttta
    10801 aagtacttat ctgctttttg aagtactcct ctagaacata cttttttatt atcatgtatt
    10861 aaaaatgcag gtatctttaa tgttccaaca ggtagaccta cttcttcgtc tatatattcc
    10921 atattgtaat ctacatacca atgacaactt ttatctggca ttaaatctac tttcgcatta
    10981 tttaatgtag ctatattata aagctcaaca cttgaaccat cagtctgtat agctgattcc
    11041 aaaaatttat ctatatcctc caaaaataat tctacaggaa tcttttcatc tgttgcattt
    11101 cctcctaaat caacccccat taaggaaaca aatcttatat ttttattgct ttttaggatt
    11161 tctcttaatt cttcattagt gtgtttatct ttctctatta cgtataatag actatccatt
    11221 tttccactct cccataattt ttatgtatat tttattttgt taaatacaag ataaatttca
    11281 ttttccttac atttatgaat ttttcttgca aattattcgt gttttattcg tgtttttatt
    11341 tatattatta tatatcaagt ataaaaagtt cgcaatattt tttattggaa aaaatattat
    11401 acatacaata gtttaacatt tttacaaata tggggtatac taaaataaaa gtatttttat
    11461 ttgaaaattt aattttattt ataatataat ctatagtaaa agaaataata aaaaaattta
    11521 ggagataaac tttatggaat acataatttt cgatttggaa tttaaccaag gatttgataa
    11581 aaaactcaat aaaactgtat ccaatgaaaa gtgtcctttt gaaattatac aaataggagc
    11641 aattaaattg gattctaaat tcaatataat agatacgttt aatagctata taaaacctac
    11701 aatttataaa gacatacatc cctttattag tcgtatgact aatattaaaa attctgattt
    11761 taatgattcg cctactttcc ctgaggtcta caacaatttt ataaaattta tatcatcaca
    11821 agaccctata ttatgtgttt ggggagctgg agatttaaag gaattataca ggaatatcaa
    11881 ctatcacaag ctaccatcaa attccttacc taaatcttat attaatatac aacaacatgc
    11941 atctaaatat ttcaataatc cagcaggaaa aagcattggc ctacaaaatg ctatttctat
    12001 attggaactt gatgaaaaaa tgtcttatca caatgcttta aatgatgcat attatactgc
    12061 taaagttttt attaagatat acaatccaag tatagtccca gatatatatc tttatacgag
    12121 tataaaacct aaaactataa gatattctaa caaaaaaaga gttgattatg ataagttatt
    12181 tgatgaattt aggaaaattt taaatcgtga attaactaaa gacgagaaaa aaatcattaa
    12241 cttagcttat aatatgggga aaacaaatca atttacatta gaaaatgtta agcaaagaaa
    12301 aaacaaatag agtttattta gataaaaaat aggcatgtag attaaaaaac tagttaactt
    12361 taaatttata tctacagccc aatttttatc ataattaatt tttattttat ctctaattca
    12421 actggacaat ggtctgaacc taatatttca gtgtgtatat ccgcacttac taatctatct
    12481 tctaattttt tggatgcaca aaaataatct attctccacc ctgcattatt ttttcttgca
    12541 ttaaatctat atgaccacca actataaaca ccttctttat caggattaaa atatctatat
    12601 gtgtctataa acccactgtc taataattta gaaaacttct ctctttcttc tttagtaaaa
    12661 cctgcatttt tcatattatt tttaggattt tttaaatcta tttctttatg tgcaacgttc
    12721 atatctccac acacaattac aggcttatga ttatctagtt tggttaaata atctataaag
    12781 tcgtcttccc atctagttct atattctagt ctctttaatt cagattgaga atttggcgta
    12841 tatactgtaa caaagtaaaa atcttcaaac tctagagtta tcactctacc ctctttgtca
    12901 tgttcttcta tatttattcc atacattact tttaaaggct ctttttttgt aaatatagca
    12961 gtacctgaat aaccctttct ctcagcataa ttccaatatt gaaaatatcc tggtaaatct
    13021 aactctattt gcccttcttg caatttagtt tcttgtaaac aaaatatatc tgcatctact
    13081 tctttgaaaa agtctaaaaa tcctttccca acacaagctc ttattccatt aacattccat
    13141 gatataaatt tcatacttat actcctcatc taaacattga cacactcccc atagctaaag
    13201 atgggggctt ttcaacaatt ttattgtaaa ttcttaattc acttttattt taacaatgct
    13261 atactataat ttcatctaaa attactattt acttaatacc taaaaatata tgttttaatc
    13321 aatttttaac ttttttgtta aatcttcagg gctcattgaa gaaacataca tatttgttcc
    13381 aagttcaaat cctccaaaat tatattttct tgggattatg tgcatatgaa cattaaaata
    13441 cgattttgtt tcaatctttg ttggatgagt gtgaatacat aagttaaatg gcatatatcc
    13501 attgatattg tataacttct taaacaactt ttcaaatatt gtagaaagct cctgtatatt
    13561 attatcattt aactcaccaa actctatttt atctttaaat attattctta cttctccact
    13621 atacttggtt gcatatggta taagtaccaa aaaagcttct ccattataaa caactcttct
    13681 atctaaactt atttcatttt ctattaaatc atcatacaaa gagcttttat tgctgtcata
    13741 atactccttt gaaaccaaca gttcattcgt aatttcaggt ggaataaaag acatagacaa
    13801 tatttgagca tgtggatgca tcaaagatgc tcctgccttt ctcaagaagt ttttgaagat
    13861 actaacatac tctactttat catctttcga caaatctcta aatctatttc tatacataaa
    13921 aaaaacatct tcaaattctt tttgactcat gttataaaaa ctacgattat gtttatcgct
    13981 ctctatcatt acttcatgaa caccatatac atctcttgcc atatcatcaa ttataggaaa
    14041 tttattgtag acagatttta caagccatcc ttcttcacta tctatttcaa ataatgcatc
    14101 agttgcatga gcttcatttc ctttgcaaaa tgggcattct tcatcatatt cattagtttc
    14161 ttcatcttca tttgggatat ctgctgtatc taaaggtctt tttaacctat ctgtagcaaa
    14221 tataacaaca tcattggtaa ttggatctat tcttaattct ttcataatta acacctctta
    14281 gttaatagcc tttaatataa ttatatgata tttatcgtat ctgttaaata aatttataat
    14341 atttttta
//
LOCUS       DACXMZ010000086        14182 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.087, whole genome shotgun sequence.
ACCESSION   DACXMZ010000086 DACXMZ010000000
VERSION     DACXMZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14182
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.087"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            195..2072
                     /locus_tag="JD016_10225"
     CDS             195..2072
                     /locus_tag="JD016_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit EIIBCA"
                     /protein_id="HAU5000011.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            2429..3835
                     /locus_tag="JD016_10230"
     CDS             2429..3835
                     /locus_tag="JD016_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAU5000012.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            3863..4711
                     /locus_tag="JD016_10235"
     CDS             3863..4711
                     /locus_tag="JD016_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU5000013.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            5372..6064
                     /locus_tag="JD016_10240"
     CDS             5372..6064
                     /locus_tag="JD016_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000014.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCAKHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            6061..6996
                     /locus_tag="JD016_10245"
     CDS             6061..6996
                     /locus_tag="JD016_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU5000015.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            7144..8067
                     /locus_tag="JD016_10250"
     CDS             7144..8067
                     /locus_tag="JD016_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000016.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            8060..8821
                     /locus_tag="JD016_10255"
     CDS             8060..8821
                     /locus_tag="JD016_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000017.1"
                     /translation="MLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNLPFE
                     QLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLAVLL
                     FISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYFNRS
                     YIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEYTAL
                     RQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            8831..9613
                     /locus_tag="JD016_10260"
     CDS             8831..9613
                     /locus_tag="JD016_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000018.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            9990..10526
                     /locus_tag="JD016_10265"
     CDS             9990..10526
                     /locus_tag="JD016_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HAU5000019.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            10558..10947
                     /locus_tag="JD016_10270"
     CDS             10558..10947
                     /locus_tag="JD016_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAU5000020.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            10984..11370
                     /locus_tag="JD016_10275"
     CDS             10984..11370
                     /locus_tag="JD016_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HAU5000021.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            11497..12939
                     /locus_tag="JD016_10280"
     CDS             11497..12939
                     /locus_tag="JD016_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HAU5000022.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            13236..14051
                     /locus_tag="JD016_10285"
     CDS             13236..14051
                     /locus_tag="JD016_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAU5000023.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIHSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIITGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         5117 a         1733 c         2697 g         4635 t
ORIGIN      
        1 cgcttatgat actataaagt taacaaattt gaatatataa cagtagacgt gttactggta
       61 atgcaggcaa gatcgaatca atttttatag attatctata gagtactatg ggtataatct
      121 atttatttgg ttcgattttt ttgtttaatt ggccataaaa caaaaagcct atagttgaaa
      181 atggaggatt ttttatggat tataaaaata tagctcaaga aattctacta aatgtaggag
      241 gaaaagagaa tgtaaatgaa gtgactcatt gtatgacaag acttagattt aaagtcaaaa
      301 gcgcttctaa agtgaataag gataaattat ctaaaactga aggagtaata actgttgttg
      361 agagtatggg tcaaatacaa gttgtaattg gaaataaagt aaaaaaagta tatgatgagg
      421 ttataaaaat tgtacctcaa tcaaataaca taggtaaaaa gaatgaaagt caagaaaagc
      481 aaggtataat taattctata ttatcagcag tagctggtat atttacacct acaatacctg
      541 ctatagcagg ggttggtatg attaaaggta ttttatcagt tcttgctatg tattatatga
      601 ataaaaatgg agtagatata aaagaaacgc aaagctatat tatcttaaat gctatggctg
      661 attctatatt ctattttatg cctattatac taggatatac tgcggctaaa gtatttaatg
      721 caaataaaat aatatctatg gtattaggag ctacactttg ttatccaact tttactgctt
      781 tgatggcagg tgaagaatct gttagatttt taggattggc agtaacaaaa gctacgtata
      841 catcatctgt tattccaata attattgcta tatgggctct ttcatacgtt gaaaaagtat
      901 tagaaaagta tatacctgag gtaattaaaa taataatggt tccaacttta tcattggttg
      961 taatgttacc tgcaacatta tttttatttg gaccaatcgg aatttatata ggaaatgtca
     1021 taaacttttc ttataagtat atttatgaat taagcccagc attgtgtgga gcatttgtag
     1081 gtgggttatg gtgtgtgttg gttatatttg gtgctcatag agcattatta ccaataggta
     1141 taagtgatgt tgctcaaaca ggaagacaga atttattggc atttgcagga gctgcaaact
     1201 tttcacaagc aggggctgct ctaggagtat tctttaaaac taaaaatcaa ggactaaaaa
     1261 caatttctat gtctgcaact ataacagctt tatttggtat aactgaacct gcaatatacg
     1321 gtgccaatct tagattaaaa aaacctatgg tatgtgccgt aatatgtggt gcatttggag
     1381 gtgcgataat gggaatgggt ggagcttatg gtaatgcatt tgcaaaccaa ggtgttttaa
     1441 ctatacctgt atatgctgaa gcaggtgcat taggtttctt aagttatcta ggtggttgtg
     1501 ctatagcatt ttttggttca gctatttcaa cttatctagt tggatttgaa gatttggaag
     1561 agtcttctaa taaggaaaat tcatctataa aagtagaaac gaaggatggt gttattgata
     1621 taacttcacc tgtagagggc gaatgtatag aattgtcaga agtaaaggat gatgtatttg
     1681 cttcaaaagc tatgggagaa ggtatagctg tgttacctac aaaaggagta ataacagccc
     1741 ctactgattg tgaagtagct tctttatttc caacattaca tgcaatagga ttaaaattag
     1801 ataatggagc agaaatgtta atacatgttg gaatcaatac agtagagtta aatggtaaat
     1861 actttacaaa acatgttaat caaggagatt tagtgaaaaa aggtgataaa cttatatcgt
     1921 ttgatataga taaaataaaa aaggctggtt atgatgtaac tacgccagtt atagtaaaca
     1981 atacatttga ttttgggcaa gtagtttctt gtaaatctag ttatgtttca accaatgata
     2041 atataatttc attagtatta gctggtaatt aagttaattt gactagagat aagtaaattg
     2101 atttaaaagg aaataagtaa atcaacaatt aaatacatct aaaagtaagt ttattgcaat
     2161 gaattgttaa agaaaataat taatatggag taaagctatt gcaatgaagt aacaaagaaa
     2221 ataattaata tagagtaagt ttattgtaat gaattgttaa agaaaataat taatatagag
     2281 taagtttatt gtaatgaatt gttaaagaaa ataattaata tagggtaaat ctatctcaat
     2341 gaaataacaa agaaaagagt taatatagag tgaacttatt gcaatgaatc aacaaagaaa
     2401 ataatcaata taaaaaggag aaatatttat gaatacagga tttccaaaag actttttatg
     2461 gggagcatca tcttctgcat ttcaagtgga aggagcttgg gataaagaca ataaaggaaa
     2521 aaccgtagcc gactataatt cttttaaaaa gtctcatttg caggcagata ctaaagtcgc
     2581 atcagacttt taccataatt atgaagaaga tattgaatta atgaaagaat tagggatgaa
     2641 aacatatcgt ttttcaattt catgggcaag aataatacca gatggtgaag gagagataaa
     2701 ccagaaaggt ttagactttt ataataagat aatagacaaa ctaatagagt gtgatattga
     2761 gccattcgta actttatacc attttgacct tccatttaaa ttagtagaga aatataatgg
     2821 ttgggaaagt agagaaactg tctatgcttt tgaaagattt gctaaaatat gctttaagca
     2881 ttttggagat agggtaaagt attggcaacc tcataatgaa cagaatttaa ttgttagagt
     2941 ggaggagaga attaatatat atgatgaaac agattcatgg aagatagata aaatgcgtgc
     3001 tcagatggat tataatcttt gcttagcaca tgcattagca gtcaatgcat gtcatgagat
     3061 gataaaagaa agtaaaattg gagcagcagt atcatcatct gtaacatatc ctcttacttc
     3121 taagccagaa gatgtatatg cagctagaat gaatgataat tttaaagtgt actatatgct
     3181 tgatatgcat cattatggag aatatccagg gtattatatg aagtatcttg aaaaaagaaa
     3241 tatagttcct cacatggaag atggggataa agagatactt aaaaaagcta aaatggattt
     3301 tatagctgta aattattata ggacaaattg tgcagaagct ttacctgaag attcacaaca
     3361 tccatttgga ttaagagaag gaactgttga ttttagtatg tatggactat ttaaaatgtc
     3421 aatgaatcct aacttagagg cttctgagta tggagcagca atagacccat caggtcttag
     3481 agttgcctta aatgaatatt ggcaaagata tcacttacct gttataataa cagaaaatgg
     3541 tctaggagct aaagatatat tagaagatgg aaaaattcat gatgactatc gtatagacta
     3601 tttaagaagt catataaatg catgtaaact agctattgaa gatggtgtag agatgattgg
     3661 atattgtcca tggtcattta cagacctttt aagttcttct caaggtttta ataagcgtta
     3721 tggacttgtt tatataaata gaactgacca tgaagtttta gatttaaaaa gaatcaagaa
     3781 agatagtttt tactggtata aagaagttat agagaacaat ggaatagtaa aataaacata
     3841 aatatatatt gagggtttta gtatgtacat tacaaaggta ttaaataatt cgttgctact
     3901 tgcaaaggat gataatgatg aagaaataat attgatgggg aaaggcattg gccataatta
     3961 taaggccggg tctgagttaa ataaaaaaga tatagaaaag atttttgttc ttcatgatga
     4021 aaatataaaa aagagtatta tacaattggc aacagaaata gatgcagaat attttggtat
     4081 agctcaaatg ataattgcat atggaataga aaaatataac ttaaaactta tgaaccatat
     4141 atatttggcc ttaactgacc acattgcctt tgcagtgaga cgttttaaag aaggtataat
     4201 gattgaaaat cactatttgt tcgaaatcaa ggaatttaat ccaaaagagt atgatatagg
     4261 aaaatatgct attactgttt ttaaggaggt tcttggttta gacctaccag aagaagagat
     4321 tggtaatata gcaggccatt ttataaatgc acaacaagat aatccttata gtgatagaaa
     4381 taagagaagt gctaaaattg taaatgctat tttgcaaata gttcattatc acttttctat
     4441 tgtatataat aaagagtcat tttattatag gagatttgta atgcatctaa aggccttttc
     4501 acaaagattt ctggctaatg aaccatcaag agaaaaaata gattttatat atgagcaagt
     4561 tcaaaaaaat tgtaaggaag agtatgaatg tgtgaaaaag gtaggagctt atatgtataa
     4621 ggaatactcc agagaattac caagacaaga ggagttatat ttgatgattc atattcataa
     4681 gattttagga gaattatgcg aaaatagtta gaatatctta atatgctagt aatattataa
     4741 aaaattaaaa attaactgca ctttaaatat gaataattgc attacttaag ggggttgtct
     4801 taaaattgat ttttttaatt aaatttgaag ctcaactcct tttttaaacc tttatatcat
     4861 tacaacttta atgtttatac gactgtactt tagatgtata tctacttttt acatttctca
     4921 acatgcaaac ctaccatttt ttgcagtatg ttaattgaaa taaaccattg agttaaccct
     4981 aaatttattc tggacttata ggaatagttc caaagatact ttaaaatatt ttctggaata
     5041 attgcttatt tcacatatgt tttattgtga tgttttgatt taataaattc ttcaactctg
     5101 tatatagtaa atgtattatc atgtttgttt tattcaagca cacttataat acgcttgatt
     5161 ttttcacaat gatgaaatat actaatgggt gttataggta taagaatagc attacacttg
     5221 aagaagtaag tgataaatga gatataaatc ctcatatgat aaactactat ttcattgagg
     5281 aacatattct aagtgatgtc aagatactac aatatgacta aattcctagt gataaaaaag
     5341 tctacgaatg agtgtagaaa ggggaactaa gatgaataaa gttttaatta tagatgatga
     5401 taaagatctt tgtattttgt taaaaaagag tatttcaatt gaacagattg aagcaaacta
     5461 ttgccattct gggagagatg gactggttga actttcaaaa caagattatc agttagtagt
     5521 tctagatgtg atgatgccag gtatggatgg ttttgaaact atggaacaaa tacgaaaata
     5581 tagcagtatt cccatcttaa tgctgacatc aaaaagcgat aattacagca aagtgcatgg
     5641 attgcgagct ggtgcagatg actatctaac caaaccattt gaaattacag aatttactgc
     5701 tcgtgtactg tctttaatcc gacgttacac ccgttttaat ccaaaaaatg aggagccaca
     5761 gcaactttct ttcaaaggtt tgttgattga ccttgaccag catacagtga ctaccaatgg
     5821 cgaacctatg ttgcttccac ccaaggaatt tggtgtgctt ctttactgtg caaagcatca
     5881 gggtaagatt ttgacaaaac agcagattta tgaagccgtg tggggtgagc catatgtata
     5941 tgatgacagc aatatcatgg ctattatcag tcgcctaaga aaaaaaattg aactagaccc
     6001 atcatctccc atctatttgc aaacaataaa aggaattggg tatcaattca acacggaggt
     6061 atgaaaatgg acgaacaaac gataattctt ttcttactct tgctgtcgat tggtctactt
     6121 tttctttcca tatttgcttt ttccaagctt gggcatattt acaagcgcct aaaagatata
     6181 gaggagatac tggctgatgt acaaattgga gacgaaaatc gtaaaatact aattaagcca
     6241 tgtgatgtta tggctccatt ggtttatcag cttaatgaga ttgtctacga ttatgaaaat
     6301 aagttacttt ctctgaaaaa gtcggataaa gctagcaaac aacttatgac aagtttatct
     6361 catgatgttc gtacaccatt aacaactctg attggttatc tggatgctgt acacagtggt
     6421 atagttatcg aacaagaacg agaggaatac cttgaaatcg ctaggcgtag agcctacgac
     6481 ctcaaggatt acattgatgt tctttttgac tggtttcggt tgaactctga tgaatttacg
     6541 ctgtcaatag aatctgtaga aattgcagag ttgtcacgca atattttgaa agactggatt
     6601 cctatattcc atgaaaagaa actgaatttt gaaattgata ttccagaaaa tcgactaatg
     6661 gtaaacctcg acccagatgg atattcccgt gtggtcaata atctggttca aaatgttctt
     6721 gctcacagta aggctcggca aatcaagatt accatgagtg aggattccag aatggtatta
     6781 ctccgtgttg aggatgatgg tgttgggatt gccagagaaa atttaccaca tatttttgag
     6841 cgactgtata agtgtgataa aggacgttca gaaaaaggaa gtggtcttgg tctatctatt
     6901 gtcagtcaaa tggtggagcg aatgggtggt caggtttccg tagaaagtga gattggaaag
     6961 tataccgtat ttacagtgag cttaccatta atataaaatt gtttttcccc catgtgacat
     7021 gtatacacag ggggattttt tgtaagttat aaagttgcaa ggttaataca aggttgttgc
     7081 aaggttaatg taagattgag gtggtatgat aactttaata aacaaaggag gcttttatta
     7141 tttatgaata atttaattat tgaaacaaaa aatctcacta agcaatatgg tgctcaaaag
     7201 agcgtttcta atttgaatat tcacgttcag aaaggtcgca tttacggatt gctaggaaga
     7261 aacggtgcgg gtaagacgac aactatgaaa atgttattgg gtctaacaaa accaactact
     7321 ggagaaatta aaatatttgg tcaggacatt cacaataacg agaagaagct tcttcctcga
     7381 attggatgtt tgattgaatc accagggttt tatcctaatc tgacaggaac agaaaatctc
     7441 aaaatctttg caaggcttag aggaatacca aaaaaaaatg cagtagaatc agcactggat
     7501 gtagtcggtt tgccaaacaa tgacaaaaag ttattttctc aatactctct tggtatgaaa
     7561 cagaggttgg cgattgctct tgccattatg catgacccac aactgttgat tttggacgag
     7621 cctatcaatg gacttgatcc tattggtatt gcagaagtaa gaacgtttat tcgtgatctc
     7681 tgcacagaac gaggtaaaac tattttgatt tccagtcaca ttctttctga aattgccttg
     7741 ttggcagatg acattggcat tattgaccat ggtattttgt tggaggaaga aagtcttgcc
     7801 caattagaag aaaaaaacag taaatacata cattttgctg tatctgatgc agcacaggca
     7861 gcaaggcttc tggaaaccca acttaacctg aaaaatttca aagtagcaga taattacaat
     7921 attatgctat atgatacaga tgtttctgtt gcagaaataa atcgtgcttt cattctaaac
     7981 aacattgagg tatcggaatc tcatttatgc gaagatactc ttgaagatta cttcaagaaa
     8041 gtaacagggg gtgaaggaat tgcttgatat aatttcatgt gaattttcaa aactcaagcg
     8101 gttaaaattt atactgattt ctattttagg tgcttgctta tttccaattc ccgcaacaat
     8161 tttgattgca aaggacaact tgccctttga gcaattgttc aaattagttg tcaattttgg
     8221 atattttttg ctattaccaa ttgtattaag tattgttgct agccaactat tttttataga
     8281 aagagataat gatgtattaa aaaatcttgc aacagtgcca gtgcaaaaag gaaaactggc
     8341 tctggcaaaa ttggctgtgt tgctgtttat atcattgttc tactctgttg caggattagg
     8401 agctactatc attggaggtt tcattgttgg aatagtagag ggggttgctg taaaattagg
     8461 aatgagcatt gcttttggaa ttatgttttt tgttactgta cttcctgttg tggtactcat
     8521 tgtatatttc aatcgcagct atattttttc tattatactt tcttttgtat atgctatttt
     8581 caattttagt atatccttaa atattatcaa ttttgaacca aataacccaa tattaagtgt
     8641 attacctgct cctgttataa tgcgttggtg gatggcattt tggggagatc ccactggcga
     8701 atacacggca cttagacagc catatttgct ctcaactcct gcttgtgtag gtattttatg
     8761 tttgataacc attatagcag tcctgctgat ttgtatgata tacaaaaagc aggagaatta
     8821 ggaggtatct atgcttgata taattactac agaatttcaa aaaataaaaa gatataatat
     8881 tctatggatt ggtgttgttg ctgttctgtt ttcagcacta ttggcagttt ttcaggttgt
     8941 aagttcacac ggcagtgacc ctctaacgta tgagagcttt gcaaacggag ttatctggaa
     9001 taatttcagt cttagttttc catttgggat tacacttatt ggaggatatc ttattaaccg
     9061 tgaatataca gaccaaacct taaaaaacat cctaactgtt cctatttcat tacgcaaatt
     9121 gctagtggga aaattaattg cggttggagg aattactgta ttgttaagtc ttttcagctt
     9181 cttatgcaca tttattttgg ggttgttgtt ttgcaatatc aatatatctg ttacactcgt
     9241 tgtaaaatcc ttagttcaga ttttgtctgt taatctgtat tgttatgttg ctgttttgcc
     9301 gattattgct tattttggtc gtaaacaaaa tgcatttctg acgggtgtag ggattgcatt
     9361 tgtatacggt ttttgtggtg tgtttgttgc tggaagaaat ctgacagact tttatccaat
     9421 cacagcaggt cttggtctag ttggatacaa caatggtgct ggtactgttt atcagccact
     9481 tatcggtgct atgactttga ttgtaattct aatcttgaca actattctac ttgtatttac
     9541 acctaattat gataaagtta tgaccatatc taagaaaaaa gggataaagc ataaaaagta
     9601 ttctcataaa taacataaat attgtttcta ttatttaagt tgctagacag tgaaaagaaa
     9661 aaatctgtcg tacagtgtct atatgctcat gtattcaaga agctacagtt ttgatttttc
     9721 ataactgtag cttctttttg tatttattaa atcacgacta tcataatact tgcaataggt
     9781 atagcgaaaa gagagagtac tgtcatatgt gttttatata ttacattaag tatattctaa
     9841 aatatatgtt ttaaataata tatttgaaaa taggaatatt taaataaaaa aaataatatt
     9901 atatatagtt gacaaaaatt ggcaaatgag ataatatata aatatagaaa tagtaatcat
     9961 tactattatt aaaaagggag gaattaatta tgaacttaaa aggaactaaa acagaaaaga
    10021 atttaatggc aggatttgca ggagagtcag aagcaagaaa taaatataca tactacgctt
    10081 caaaagcaag aaaagaaggt tataatcaaa tagctgctat atttgaagaa actgctaaca
    10141 atgaaaagga acatgctaaa ttatggttta aacttttaca tgatggtatg ccttcaacag
    10201 aggaaaattt aaaggatgca gcagcaggtg aaaactatga atggactgat atgtatgcaa
    10261 aatttgctaa agaagctaga gaagaaggat ttgacaagat agcttattta tttgaagctg
    10321 ttggaaaaat agagaaagag catgaagaaa gatacttaaa gcttttagaa aacttaaatg
    10381 aaggaaaaat atttaaaaga gatgaagaag ttgtttggca atgtcaaaac tgtggtcatg
    10441 tgtatgtagg tactgaagca ccagaaaaat gcccagtatg tgatcatcca aaagcgtatt
    10501 tcaatataaa agctgaaaat tattaaaaat tatcattatt aaaaaggaat gttaactatg
    10561 aaattttcta aacaacgaga actgatttta aatgaaatat taaataatcc ggttcatcct
    10621 actgcggatt acctatatga aaacttaaaa aaagataatc caaatttaag cttaggaact
    10681 gtgtatagaa atttagctca gctaacggaa catggcttta taagaaaggt tagtattcca
    10741 gggtatccag atagatttga tggtcgaata gataatcact atcatatcat atgtgaagta
    10801 tgtggagagg tatacgattt agaatccgaa gttcttaata atttacaaga attaatatct
    10861 gaggagacag acataaaaat aacatcttat aacataagtt ttaaagggat ttgtaataat
    10921 tgtaaaaggt gtagccaagt aggttagttt tcaaattcta aaaataatta atgaggtgag
    10981 tttatgtgta gtgaacaaaa attttttata tgtgagactt gtggaaacct tgtaggaatg
    11041 atacaaagtg gtggtgtacc tattttctgt tgtggaaaac ctatgaagga acttgttcct
    11101 aatacaacag atgcagcagt tgaaaaacat gtacctgtaa ttgaagtgga tggaaataat
    11161 gttactgtta aagtaagtag cacaactcat ccaatgacta aagaacatca tatagcatgg
    11221 gtgtatctta tgactgaaca aggtggtcaa cgcaaatgtc tagcagtaga tggagaacct
    11281 gtagttaaat ttgctttgaa tgatgatgat aaagtgattt ctgcctatgc ttactgtaat
    11341 ttacatggat tatggaaggc tgaactgtaa tatttaaata aattgagtca aaaaggaggc
    11401 tataaatgta ttttcataca aatagcctcc tataaattta tataaattca agttttaatt
    11461 ttgtttaatg tcaatataat ataaggagaa aataatatgt caatttataa atgtagtgtt
    11521 tgtggatata tttatgatga atctaagaat gataaaacat gggatgaatt aagtgaagat
    11581 tgggaatgcc ctgtatgtac aaaaggtcgt agttattttg gaaagataag tactgtttac
    11641 tatgaagaag atgaaaagat agcagaagac atagttgaag atgaatctaa attaaatact
    11701 gaaaaagagg gagatttaaa ttacttaagt acatatttaa gaagagatga tgaagttgag
    11761 aaacatatgg atataattca cgaaatggct gttacaggaa aatctataat agaacctatg
    11821 agaactaaac taccagtaat ttcttgggat gatattttaa ttatgggtgc tcaattaaat
    11881 ccattacctt taaatgagca tgatgaagta aatacaacaa caataatagg aaaaaaagca
    11941 aagaaaccta tgattataga aaatccagtt tatatttccc acatgtcatt tggagctttg
    12001 tctaaagaac taaaaatagc tttagcaaag ggtgcagccc aaaataagac agctatgtgt
    12061 agtggtgaag gaggtatatt accagaagag aaggaagctt catacaagta tatttttgag
    12121 tatgtaccaa ataaatatag tgtaactgaa gaaaatctta aaaattctga tgctatagag
    12181 ataaaaattg gtcaaggaac aaaacctggt atgggaggtc atcttcctgg cgaaaaagta
    12241 actgaagaaa tagcaaaagt aagaaataag cctgtaggtc aagatgtaat aagtccttct
    12301 tgttttgagg aaatacaatc caaagaggat ttaaagaaac ttgtagatga acttagagaa
    12361 gtttcagaag ggcgtccaat aggtgttaaa atttcagcag gtcatattga aaaagatatg
    12421 gaatttatag cttatgctaa acctgatttt gtaactattg atggtcgtgg tggagctact
    12481 ggagcaagtc caaagttgct taaagatgca acttcaattc caacaatatt tgctctttat
    12541 cgtgctagaa aatacataga tacacatgga ttagatatag atttggttat aacaggtggg
    12601 cttcgtatat ctactgattt tgctaaggca attgctatgg gggctgatgc agttgctatt
    12661 gcaagttctg ctcttatggc agcggcgtgt caacaatatc gtatatgtgg ttctggtaag
    12721 tgtcctgtag gagtagctac tcaggatgag gaattgagaa aaagattaca tatagaaaac
    12781 agtgctaata gagtggcaaa ctttttaaat gtaagtttag aagaacttaa gacttttgct
    12841 agaatttctg gtcataaaga tatacatgat ttaagtgttg atgatttata tactgttaac
    12901 tctgaaatat caaattatac aaacatacag catgtataga ttgaagtttg aaactattag
    12961 attttagata actataaaag taaatgataa atatatttta taaaagagag tgtcttaaaa
    13021 tgaacttttt agttcataag tcactctttt ttgtatgaag ccaaatatat ttatcatttt
    13081 agcaatgaaa aagaatctta tctctttatt ttgagataga ctccttctat ttatgaaaat
    13141 taaagatatt aaattgtaaa atattctgtg atgtcgtact attaaactaa agattctgta
    13201 ctttaaagta aataaaaatt taaggagtga ttattatgga aagattgaag atacaagttt
    13261 tagtcgacaa taatacttat attgatagat actttgttgg agaaccagca gtaagctatt
    13321 atattgagat tgatggaaat aggattttgt ttgactcagg gtattcagat gtattcatat
    13381 caaacgctga aaaattaaat atagatttag gaaatttaac tcatgttgtt ttttctcatg
    13441 ggcataatga ccatacaaga ggtatccagt ttctagaaaa taggtatgat ttatctacag
    13501 ttgaacttat ttcacatcct aactgtttta tacctagaaa acatggagag aaaagcatag
    13561 gagctccatt ttctgctgag gaaattaaaa atatttttat gtataatcct aaggataagc
    13621 catttaatct aagcaagaat tgtgtgtttt taggtgaaat acattctata aatgattttg
    13681 aaaaaagagc taaaattggt aaatgtaaaa taggagattt gtgggaagac gattatgttt
    13741 tagatgattc tgctatagtt tgtaaaactg ataaaggtat ttttattatt acaggatgtt
    13801 ctcatagtgg aatttgtaat atagtagagt atgctaaaaa ggtatgtgga gatgatagag
    13861 ttattggtat actaggtggt tttcatcttt ttgaattgaa taataggtta gatagtacaa
    13921 tacaatattt ggataaagaa gaaataagaa tgttgtaccc ttgtcactgt gtttctttta
    13981 aggtaaaagc gaagatgaat gaaatattga atataaatga agttggagta gggctgacca
    14041 taagtatata gtatttgaaa gtatataaga tttaagttga aggttaaaaa gatattttct
    14101 atagaaaata tcttttttgt tacgtttatt ttaagagaaa tcaatatact tttatatgaa
    14161 atattgtaaa aaaatttatt tt
//
LOCUS       DACXMZ010000087        14138 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.088, whole genome shotgun sequence.
ACCESSION   DACXMZ010000087 DACXMZ010000000
VERSION     DACXMZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14138
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.088"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            72..2183
                     /locus_tag="JD016_10290"
     CDS             72..2183
                     /locus_tag="JD016_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HAU5000024.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVLINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(2381..3346)
                     /locus_tag="JD016_10295"
     CDS             complement(2381..3346)
                     /locus_tag="JD016_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAU5000025.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLN
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(3631..4587)
                     /locus_tag="JD016_10300"
     CDS             complement(3631..4587)
                     /locus_tag="JD016_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAU5000026.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            4955..5743
                     /locus_tag="JD016_10305"
     CDS             4955..5743
                     /locus_tag="JD016_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAU5000027.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(5866..6336)
                     /locus_tag="JD016_10310"
     CDS             complement(5866..6336)
                     /locus_tag="JD016_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HAU5000028.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            6475..7119
                     /gene="uppS"
                     /locus_tag="JD016_10315"
     CDS             6475..7119
                     /gene="uppS"
                     /locus_tag="JD016_10315"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HAU5000029.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDITLGG"
     gene            7162..8067
                     /locus_tag="JD016_10320"
     CDS             7162..8067
                     /locus_tag="JD016_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAU5000030.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            8382..9929
                     /locus_tag="JD016_10325"
     CDS             8382..9929
                     /locus_tag="JD016_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAU5000031.1"
                     /translation="MNKNSMTKIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVNSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLVDK
                     CMTVEEE"
     gene            9932..10330
                     /locus_tag="JD016_10330"
     CDS             9932..10330
                     /locus_tag="JD016_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAU5000032.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            10370..12103
                     /locus_tag="JD016_10335"
     CDS             10370..12103
                     /locus_tag="JD016_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAU5000033.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            12335..13546
                     /locus_tag="JD016_10340"
     CDS             12335..13546
                     /locus_tag="JD016_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAU5000034.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            13739..13999
                     /locus_tag="JD016_10345"
     CDS             13739..13999
                     /locus_tag="JD016_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAU5000035.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
BASE COUNT         5695 a         1486 c         2304 g         4653 t
ORIGIN      
        1 atgaatttta gatatactta ataagaagta tattgtagaa aaaataaatt taaaaaatta
       61 agggggatga aatgaaaaaa ataagtatat tagtattatc attgataatg acacttacta
      121 tgtgtagtgt aagttcattt gctgattcat caaatgataa ggaaatgaga gctgcttgga
      181 tatctacagt ttataattta gactggccta agactaagaa taatgaagcc aagcaaaaaa
      241 aggaatacac agatttattg gacaaattaa aaagtgtagg aataaataca gcagtggttc
      301 aagtaagacc aaaatcagat gctttatata aatcaaatat aaacccatgg tctgaatatc
      361 ttaccggtac acaaggtaaa gaccctggat atgacccatt accattttta atagaagaag
      421 cacataaaag aggaatggaa tttcatgcat ggtttaatcc atatagaata actatggcag
      481 atgagagcat agataaattg ccagcaaacc atccagcaaa gaaaaatcca agctgggtag
      541 ttaaacatgg aaataagtat tactacgacc caggtttacc agaagttaga aagtacatag
      601 tggatagtat agcagaagta gttcaaaatt acgatataga tggagttcat tttgatgatt
      661 acttctatcc aggagtaagt ttcaatgata ctgcaacata ccaaaaatat ggaaaaggac
      721 aaaataagga tgattggaga agagaaaatg taaatactct acttagagat gtaaaagcgt
      781 ctataaagag tataaagcct aatgttgtat ttggtgtaag cccagcaggt atatggagaa
      841 ataagagtag tgacccaaca ggttcagata catcaggtaa tgaaagttat gtaggtacat
      901 atgcagatac aagagcatgg ataaaacaag gattgattga ctatgtagtt ccacaacttt
      961 actggccaat tggtcttaaa gcagcagatt attctaaatt agtggcatgg tgggctaatg
     1021 aagtaaaagg aacaaatgta gacttataca taggacaagg tatatataag caaggtcaat
     1081 cgagttatgg tggacaaaat atagcaaaag aaatagtaca acaagtaaca ttaaatagaa
     1141 agtatagtga aataaaaggt agtatgtatt tctcagctaa agatatagca aatagtactt
     1201 ctatacaaaa ggatttaaag agtttatatt ctagtagtga agaaccagtt actccaccat
     1261 ctaatgttaa agtagagaaa ttaagaggcg acgaaagata tgacacagca gtagcaataa
     1321 gtaaaaaagg ttgggctaca aattcagata cagtagtatt agtaaatggg tactctatag
     1381 ttgatggtat aacatcaaca ccacttgcta catcaaatga tgctcctata ctattggtaa
     1441 ataaggataa tatacctaca tccactaaga atgagttaaa aagacttaat ccaagtaaag
     1501 ttattttaat tggaggaaat aactctattg gagataaagt tgagagtgag ataaaagata
     1561 cattgtcaaa tgtattaata aatagagttg gtggttcaga tagatatagt acatctttaa
     1621 tgatagcaaa agaattagta aaaactaatc cagtagaaaa actatatata acaagtggta
     1681 caggtgaagc agattctctt tctatagctt caaaagcagg agaagaaaag caaccaatag
     1741 tattagtatc aaaagataat gtaagtgatg aagtttacaa ttggatatca gatttaaaag
     1801 taaaagatgc ttattttata ggtggtaatc taagtatatc tgattcagta ataaataagc
     1861 tggataaggt aattacaaat gatgtatcta aaaatagaat agctggagaa aatagagaag
     1921 aaacaaatgg aaaagtaata caaaaattct atccaaatgc tgaatattca tcaatgtttg
     1981 taagtaaaag taatcaattg gtagatgcat taacatcagg tcctttagca gctaaattaa
     2041 aatcaccagt agttatgctt ggaaatagtg taacatctgc tcagaaaact gctttagagc
     2101 ctaagaaaac tactctagta tacgaagctg gagatggtat aaatcaaaac acattaaaca
     2161 ctttcttaaa cttagttaag taataaaaaa gttaatttaa aatacagagt attttataaa
     2221 tgtagattaa aatgaaaatt atcatgataa attagaaaat aaaaaagaac tatatgcatt
     2281 ttgtaatcaa gtgtacacag caacttgaaa ataattatgc gatatagttc ttttttagta
     2341 attaatctta tactttttaa taaactttat gttgaatttt ttattgttta aactctatgt
     2401 cattaaatat aaaatcgtgt aatatgtcta aagtatctgg gtcaaatcta agaacccatc
     2461 cagcttttcc atatatacca cctgtactat gaatttcatc atcaatagga aattcaaatt
     2521 gcttaatatt taagtcgccc atttttaaaa cttgagctcc taaagaaagt attgcattag
     2581 gcttcatgtt agttttaaca tatggaagaa ctgcatctaa taaacctgga tatttagtaa
     2641 caggtaaagt ttccgttttc ttgattatag cttgtataat ctgtctctgt ctgccatctc
     2701 tagcaaaggc tgtatcgttg tgtctgatac gtgcaaaaga tagtgcctga tatccattta
     2761 gagtctgctt tccagagttt ctaatgtatt gtatgctacc tttatcatca cttttattcc
     2821 atttataagt ttcaggaata aatttattta gttctcgtat ttctcctttt tcaacagtaa
     2881 cctcaacgcc accaatagca tcaattatat acataaatga ttcgaaatta acacaagcat
     2941 aattttgaat atcaatatta aaattctctt caattgtttg tattaataaa tcagcttgtc
     3001 cataagcata agcatgagta agtttttgtt taccatgacc agggatgtct acatagctat
     3061 cacgagctaa tgatgtaagt ttgatatcat tgtgtttgtt atcaatagta agtatcatca
     3121 tagcatctga ccttgaagaa tcttcatttg gtctagcatc tgttcccata agtagtatgt
     3181 taattatccc atcttcattt ttatggtcat tgttattcaa taaatcacta cttatgctcg
     3241 agtcatgaat agcagataat ttataataaa aatatccata gacacttata gggaatatta
     3301 ctaccaagaa agccagtagt ataacaaatt tctttaattt tgacaaaagt accccttctt
     3361 tcttcttttt cctgtatatt ttttaatagc atacacttaa ttatattaaa gtattgagat
     3421 atattcaata cttgtttcag ttacaaaaaa aagacaatga tataatttat aagaaaattt
     3481 atcgacctat atatatagtt aaaagtatat ataatatatt ttttaaaagt aatttatatt
     3541 ttaaaataaa aacacacccc ataaatacaa agtaatttgc atttaagagg tatgtattta
     3601 tcatcaaatt attggtttgt tttggaattt ctaatcttca accataatat ctttaaatat
     3661 gaaatcatgc attgcatcta gttcatattc ctcaaaaggt attacatagc ctgcattacc
     3721 tatttttcta ccattttcag tagaaagagg aaactccata gatttcaaat ttaaatttcc
     3781 tatacttaaa agttcttttc ctaaagatat tatttcatta ggattcatat tagtttttac
     3841 atacggaagt attgtgttaa ctagattagg atatttagtg actggtaaat ccttaacacc
     3901 atttattaaa ctctgtataa cacttctttg tcttctatct ctttcatctg tgctatcatt
     3961 ttttcttata cgagaaaatg caagtgtctg atatccattt aatttttgct caccagaatt
     4021 tcttatatat ttaagagaac ctttattgtc atttttattc catttataag tttcaggaat
     4081 aaatttattt aattctctta tttcactctt atcaacagta acagtaactc cacctaaagc
     4141 atcaactata tccataaaag agaaaaagtc aactacggcg tagttttgta tatcaagttg
     4201 gaagttttct tctatagttt ctattaataa gttagcttgt ccatatacgt aggcatgtgt
     4261 taatttttgt tctccacgtc ctggtatatt aacataagta tctctattta atgaagttag
     4321 cttaaggctt ttatttttat tatcaatagt aagtatcatc atggcatcag aacgagaaac
     4381 ttcttctcct ggtcttgcat ctgtacctaa caataatatg tttgtgatat tttcttcgtt
     4441 tttatagtca ttactactaa gcgcctctgt atttatacta tcatcttgta tagtttttaa
     4501 tttagtaaaa acatatccat atatactaac aggaaatgct ataactaaaa gggctagaaa
     4561 tataacgaac tttttgagtc ctgacaataa tatcccctac tttctaaatt ttttaattac
     4621 acatagttaa ttatattaaa aatttgttac ataagcaata aaaaacacaa taaattagca
     4681 taagtttgtc ataatataat tatttttata gtattttata gaagaaagtg tgtagttatt
     4741 attgatttta ttggaatata attgtatgaa gacttaaatt tttataaact aatgtgtata
     4801 ttgaaaaaaa tattttcctg aatgtagtta tagcatctta tgaaaggtgc aaattgtaat
     4861 ttaaaatttt tagtgtataa taagaattga aaattcaaac cgataaaaat taataaaaaa
     4921 taattataaa ttaataattt gggggaggaa atgtatgata aaattaatag caacagattt
     4981 agatggaact ttgttagatg aaaagagcga aataaatcca gagttttata aagtatttaa
     5041 aaaattaaga gagagaggga taatgttttc tgctgctagt ggcaggcagt atcaaaatct
     5101 tataaaaaaa tttgaagata taaaagatga tatgatgttt atatcagaaa atggaacact
     5161 agtagtatat aaaggaaaag aaatattatc aaatccttta aataaagaat tagtaaatga
     5221 aatcatagag acaacaagaa gtataaaagg gaaaaaaatt gtaatgagtg ggaaaaaata
     5281 cgcttatata gaaagcaaag atgaagcgtt tatacaagag gttagtactt attatgcaaa
     5341 gtttaaaatt gtagaagatt tgacgaaagt agagggagac atattgaaga tagctgtgtt
     5401 tgacttcaaa ggtgcagaac ataataataa tatctacttt gaaaagtttt ctgatagagc
     5461 tcaagtatgt atttctggag tggagtggct tgatttaact gcaaaaggtg caaataaagg
     5521 gagtgccata aaaaaagtac aaaaaatgtt agatataaaa tatgaagaaa ctatggtatt
     5581 tggtgaccag ttaaatgatg tggaaatgat gaaaagtgcc tatcatagtt atgccatgga
     5641 aaatgcaaat gaacatttga aacaaattgc tagatttaga gcaaaaagaa atactgaaaa
     5701 tggcgttgta gataagataa aagaggttat aaaaattgga tagtcatatg tatagatgaa
     5761 aaagattatt ttgaagaaat caaaaaaata tcaatgtaat tttgataaat aagtttattt
     5821 atgcgaaaat tacattgata tttaataaac atcaataata gatatttatt tttttatgat
     5881 attcagtatt ttgttttcta tttgagttgc attttcagca gaaattaaat aaacatatct
     5941 acctgccttc tcaattatag tattatttgc tatttcagaa ttgatttttt ctccatgtcc
     6001 ttctccaaaa ggtgcttgta tcatatcttt tttataattt tctagagttt tatagatttt
     6061 atctacctcg tcagttttta taacgataat atcttctaga gataattttt cagaagaatt
     6121 aattacaaaa ccttgaatta tgtcttcctc tacatcatta aaatagttaa aatcctttgc
     6181 atcaaagatt tttgattcct ttataagtaa atcagaagat tttaatgctt tttttatttt
     6241 tgatgtagaa acgtttttgt aatcaccact tgaagaacaa ctaaccattc ctaatgataa
     6301 gattatcgaa aaaaacaata ttaaaatttt tttcatgata aacctccaag ttttttaaac
     6361 tctgtcttaa attagtttac tacataatta tataaaaata aatgtattta atgttacagt
     6421 tttgattaat aatgtaatat tttgataata atacctattg agaggtgatt agaaatgaga
     6481 ataccaaaac atgtaggaat tataccggat ggaaatagaa ggtgggcaag tcaaaatggt
     6541 atgacaaaag ataaaggata tgaaagtgga atagaccctg gactagagtc ttatagatta
     6601 tgtagagatt taggctgtga agaggtgact ttttatggat ttacaactga taatacaaga
     6661 cgtccatcag tgcagacaaa agcttttaca aaagcatgta ttgatgctgc aaatatatta
     6721 atagatgaag gtgctgcttt attagttgta ggaaatacaa aatcgccaat gtttccagaa
     6781 gaattattac catatactaa tagaattaaa gccaaagaag gtcaaataaa agcaaatctt
     6841 ctggtaaatt atggttggta ttgggactta aataatatat caggagtaga aaatgtaaat
     6901 aaaaataata tacaggatta catacagtca tttgatgtat ccagactaga cttgataatc
     6961 agatggggtg gaagaaggag attaagtggt ttccttcctg tacaatcaat ttatgcagat
     7021 ttttatgtta tagataatta ttggccagat tttaagagtg acgatataaa ggacgcattt
     7081 agttggtatg atacacaaga tatcacttta ggtggataat taaaaatatt ctgatttaag
     7141 gttgtagagt gttttaaatt attgaaaaaa aaacttcttg atggtaaaat aacactaata
     7201 atatgtaaaa gtgtaaaaat ttataccaag aagggtgaag aaatgtcaaa aggcaataat
     7261 aacaataatt caagaaacaa atctaaaaag accagtcatc tgaatagaaa aaagcgaaaa
     7321 ttaaataaga aaaagttagc agtgttgatt tgctttacag ttttattttt atttattgca
     7381 tttaaggcta ctcaaggtgt agtagctttg gtaaaaagta tggataaaag taataaaact
     7441 aatcaacaac aaaatgttaa ctcagaacaa tttgaccttg gtaatgaaga ggaaaataaa
     7501 aagaaaaaat atactgtatt tatagaccca ggtcatggag gcaatgataa aggaactgaa
     7561 agcaagacca gtaatagata tgaaaaagac ctaaatctac aaattgcaaa aaaattagca
     7621 aataaattat ctaaacaaaa ggatattcaa gtagtagtat ccagaactga tgatacatat
     7681 atatcattaa aggatagagc tatcttagca aataatagtt ctgctgatgt gcttgtttct
     7741 attcatttaa atgcagagaa aaatggtaat acagcaacag gtatagaaac ttggtatagg
     7801 aataaagcta cagatggttc aaaagaactt gcacagacag tacaaagtac tatagtatcc
     7861 tatgttaaag ttagagatag gggaatagtt gaaaataact ttgaagtact tagagaatca
     7921 aatatgcctg ctatattaat agagtgtggt tttttaacaa caccttcaga ggaacaaaaa
     7981 ataataaatg aaaaatatca agaccaactt gcggaaggaa ttgttcaagg tgtactttca
     8041 tacctagatt ctaaagggaa taagtaagtt aactttactt taatgtgagg ataaatttaa
     8101 aagtttaact gtgtaaattg tattaacttt atttcgtaat gttggaaaca ttgtttgaat
     8161 ttagaaacta tgaaattaga aattatttta tctatatcta atttcatagt tttttttgca
     8221 aaaaatttat agttataaaa ttttaaaaaa taaatttagt ataatgtaat gtagttattt
     8281 atatgtgatt ttaacttgca ttgatttgct atcaattgtg ctttataatt agtatatata
     8341 aagtttaact gtaagataat ttaaaaaata ggggaattta tatgaataaa aatagcatga
     8401 caaaaattta cagtttaaat aaaataattt ttattataat tggaatttta ggaatagttt
     8461 tactcacaag acatattgag gtagatatta aagttttaat tttaatttta ttaaccttgt
     8521 ttagtataaa tgtatttatt tcatatctta aaattaagtt gtatgaggat aaaattaatt
     8581 taggattgat aaataattca gataagttta taaatattac taaagatgag aacttcctaa
     8641 aacatgttca aaattatatt atcagtaatg aaaaagaaaa ctgtgtaatg gcttgttttg
     8701 atttatgtag actcaaactt ataaatgata tatatggtta tgagctaggg gatgaagtgt
     8761 taaataatat tttaagtaac ttaaaaaatt attttggaaa agaagctatt tatggaaagt
     8821 taaaaagtga tgtttttgta ttaataataa agcttgaaaa tagggaacag aagattcctt
     8881 atttagtaaa tcttattaag aataaaattg tgatactaag tcagattgac agttttaaaa
     8941 tggatataaa tattgaggct tctattggaa tttataaatt taacaataat gaaattgata
     9001 taaaaaaagc tatagataat gctgatatgg caaggcttaa atcaaaagga ctaaagcaca
     9061 tagagtatgt agaatttgat aatgctatgg aagaagaaat acaatcaatt atgaaaatag
     9121 agagagattt attcttggct attaaaaata aagagtttgt aattcactat caacctaaag
     9181 ttaattcaag tacaggtaac attataggtt cagaggcatt gataagatgg ttgcacccat
     9241 cattaggcat ggttggacca aataagttta tacctatagc tgaaaaaaat ggtctgataa
     9301 acaatattgg tagatggcta attcaagaag tttttattac cattaacaag tggatttcag
     9361 aaggtataaa tgtcttacca gtctcaataa atctttctag agtagaacta tataaaaatg
     9421 atttagttga tttttttaaa ctgatgttta atacatataa tattccaaaa gaacttatag
     9481 aattagaaat tacagaaacg acagcactaa gagatgtaaa atttataagt gaaaggcttt
     9541 atgagattaa atctttaggt atgaatatat cattagatga ttttggagta ggtaattcaa
     9601 attttatcaa tcttaagggg ataccactag atattataaa aattgatagg tcacttatat
     9661 tggatatagt tacaaataca aaaacaaagt ttatggtgaa agcaattgtt aacctttcac
     9721 atgatttaaa cgtaactgtt atatgtgaag gggtagagga tatgcatcag gtcaaagtct
     9781 taagtgaact gggatgtaat ttcatacaag gttatgtatt ttttaaacct ttggatgaga
     9841 tgaattataa aaaacttctt actgacggaa gttatataaa tctgaaagat actcttgtag
     9901 ataaatgcat gacagttgag gaggagtaag gatgatttca gaagatgtag attattcaat
     9961 aagaatcata agagctatat ttaataatgg agaagataaa ccaatagatg ctaaaatgat
    10021 attaaaaagt gaagatatac caatactaac actatacaga ctgcttagaa agctttgtgc
    10081 aaacaatatt cttactttta acaaagaaga tggcaagaca tacatgttaa atgtgtcatt
    10141 ggatacaata agtctaagaa ctattgtaga agtaattgat ggaaaaatat atataaataa
    10201 atgtacgaga tatgtatcta attgcagtaa ttgcttaaaa ggttgtaaag taaatcgtga
    10261 attgctagaa atagaaaaag aaatttataa acttttatcg agaaaatcta taagacaaat
    10321 actagaataa aaaattttca gctatgataa taattggagg ttttcggtta tggatgatat
    10381 atctcaagat aattttctac tgtctaaaga gtatgagaat agcttagatg tagatactaa
    10441 aaaagcaagt ggaatatatt atactcctaa aataattgta gattatattg taaaaaaaac
    10501 tttaaaaaat cacgatataa ttaaaaatcc atatccacga atacttgata tttcctgtgg
    10561 gtgtggaaat tttcttttag aagtatatga tattttatat gacttatttg aggaaaatat
    10621 atatgaattg aaaaagaagt atgatgaaaa ttattggact gttgataaca tacaccgtca
    10681 tatattaaac tattgtatat atggtgctga tattgatgaa aaagctataa gtatattaaa
    10741 agatagctta acaaataaaa aggtagtaaa tgatttagat gaatctgata taaagattaa
    10801 tctattttgt tgtgatagtc ttaaaaagaa atggcgatat aagtttgatt acatagtggg
    10861 taatccaccc tatattggtc ataaaaaact tgaaaagaaa tataaaaaat ttctcctaga
    10921 aaaatatagt gaagtttata aggataaagc tgacttatat ttttgttttt ataaaaagat
    10981 tatagatatt ttaaagcaag gagggattgg cagtgttata actccaagat attttttaga
    11041 aagtctatca ggtaaagatt taagagaata tattaaatca aatgtcaatg ttcaagaaat
    11101 agtggatttt ttaggtgcaa atatatttaa aaatataggg gtatctagtt gtattctaac
    11161 ttttgataag aaaaaaacaa aagagacata tatagatgta tttaaaatca aaaatgagga
    11221 tatatgtata aataaatttg aaacattgga agaacttcta aagagtagca aatttgaaca
    11281 ttttaatata aatcagagat tgctatctga cgaatggata cttgtaaata aagatgatga
    11341 aactttttat aataagatac aagaaaaatg taaatatagc ttggaagata tagcaataag
    11401 ttttcaagga ataattacag ggtgtgataa agcgtttatt ttaagtaaag atgatgtaaa
    11461 gttgaacctt gtagatgata aatttttaaa atgttggata aaaagtaaga atataaataa
    11521 atatatagta gataaaagtg aatataggct aatatactca aatgatatag ataatgagaa
    11581 tacaaataaa aggatactag atgaaattat agggctatat aaaacaaaat tagaaaacag
    11641 aagagaatgt aaaagtggaa taaggaaatg gtatgaactt caatggggta gagaaaagct
    11701 tttctttgaa agaaaaaaga taatgtatcc ttataaatca aatgaaaata gatttgctat
    11761 agattatgat aacaattttt caagtgcaga tgtatattct ttttttataa aagaagagta
    11821 tttagataaa ttttcatatg aatatctagt tggaatttta aattccagtg tttatgataa
    11881 atactttaaa ataactgcta aaaaaatgag taaaaatata tatgattact atcctaataa
    11941 ggttatgaag ataaggattt ttagggataa taattatgaa gagatagaga atttatcaaa
    12001 gcaaataata tcaattttat taaataagtc tatagataaa ggaaaagtag aaaaattgca
    12061 gataaaaatg gataacttga taatggattc tttaggtata taagcagttg taagtattaa
    12121 aaaattataa tcttttaatt gtttatttaa atttagaaat aaaaagttag ccatataaaa
    12181 aatattaaaa atatatactt ttcaatattt ttttgataaa ataagaatga atttgtgcaa
    12241 ttttattttt ctccaataac taaaaatagt tttttgaatt aatatttgct taagaataaa
    12301 aatcagaaac gtttttgata tagtaggtga taatatgaaa ggattagttt tagaaggtgg
    12361 aggaacaaaa ggagcctacc aaataggtgc atataaagct ttaagagagc ttggtataga
    12421 gtttcaagga gtaactggca catcaattgg tgctttaaat ggagcatata tagttcaaaa
    12481 tgatattgaa gtaatggaag atatttggtt aaattatgac tatactcatt ttatgaatat
    12541 tgatagagaa acctatgaga ggtttaaaaa tgtagatttc actactaaaa atctcaatgc
    12601 agtgtttgag cttataaata aagctagaaa aaatgaaggt atagatataa gcccattaag
    12661 acaactaatg gagacaacat taaatgaaga cataataagg aaatcaaaaa aagactttgg
    12721 acttgttacg gtctggtggg ataaaaaaat taatccatat ccattgtata tcgaagatat
    12781 acctaaagga agattgattg attatttaat tgctagttcc agtttgccta tttttcaaac
    12841 gaacatgatg gatgataagt tgtatcttga tggaatgttt tgtgataaca tgccaatagg
    12901 actcttggaa gacaaaggtt atgaagatgt tgttgtaatt aggttgattg atgattttat
    12961 tgggaaaatg aatctaaatc gacatcaaaa tataaatata aaaactataa taccatcaga
    13021 atatctagga ggttctctta ataaagatag agataatgta gaaaaaaaca tccgactagg
    13081 ttacttagat actatgaaag catatgaaag atgcactgga attaagtatt attttgatgt
    13141 agagtataaa tataatgaag aatattgttt tgataaattt agaagtatga gcaatgaaac
    13201 aatacaatat atctgtaatt tattgaatat aaaaagagat gcttcactta gaaccgttat
    13261 ggaaagtgta ataccaaagc ttggtgaggt gcttggattg ccaaaggatt tttcatataa
    13321 agatttgttt tactgtatat atgaaaagaa acttgaagaa aataatataa acaggataaa
    13381 attatatgat tttaacaagg ttatagatgt tgtaaataca aatattgatt ctaatagaaa
    13441 tttagcttct aattttgaag ttagacctaa aattagtatt aaaaagaaca aaaacacaaa
    13501 attattgaca aattgtatta taaaagacat aaaaaatcaa agttaaataa aaaacaaaaa
    13561 aaaatttgta attatactcg aaaaaagtgt atattagtag tataatattt ttgtagcact
    13621 agtgggaaca aaaaatgacc ataggacaaa aaagtaccac ttagtaccat taaagtaaaa
    13681 aacaggaaaa caattttaaa aaatataaaa tgtaaagttt ataggaggat ttaaaaaaat
    13741 ggaaaaagta gttagtatca aaaatgcaag tggattacat gctagaccag caggaatgtt
    13801 tgttaagaag gcagctgaat ttaaatcaac agtagaggtt gtagcaaaag acaaaactgt
    13861 aaatgcaaaa tcaataatgg gaataatgag tttaggttta gctcaaggag aagaattaaa
    13921 gttagttgta aatggagaag atgaagaagt agctttagct gcaatggttg aattagttga
    13981 aagtggattt ggagaataga tataataatt ttttaaaaat ttgatattta gaatctaata
    14041 ttacttaaag taaagtaaca gagaagtatt aaacatatac agaccaacat ttgtagatat
    14101 ttaatttctt tgttactttc atgtttattt aatcagaa
//
LOCUS       DACXMZ010000088        14005 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.089, whole genome shotgun sequence.
ACCESSION   DACXMZ010000088 DACXMZ010000000
VERSION     DACXMZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14005
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.089"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            404..1297
                     /locus_tag="JD016_10350"
     CDS             404..1297
                     /locus_tag="JD016_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HAU5000036.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1297..2691
                     /gene="gltA"
                     /locus_tag="JD016_10355"
     CDS             1297..2691
                     /gene="gltA"
                     /locus_tag="JD016_10355"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HAU5000037.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3057..5276
                     /locus_tag="JD016_10360"
     CDS             3057..5276
                     /locus_tag="JD016_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAU5000038.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGNKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNVKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5423..6169
                     /locus_tag="JD016_10365"
     CDS             5423..6169
                     /locus_tag="JD016_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000039.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGSSIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6169..8049
                     /locus_tag="JD016_10370"
     CDS             6169..8049
                     /locus_tag="JD016_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000040.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            8168..9559
                     /locus_tag="JD016_10375"
     CDS             8168..9559
                     /locus_tag="JD016_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAU5000041.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9666..10706
                     /locus_tag="JD016_10380"
     CDS             9666..10706
                     /locus_tag="JD016_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HAU5000042.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11195..11848
                     /locus_tag="JD016_10385"
     CDS             11195..11848
                     /locus_tag="JD016_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAU5000043.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKAFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            11924..12133
                     /locus_tag="JD016_10390"
     CDS             11924..12133
                     /locus_tag="JD016_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000044.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12273..12839)
                     /locus_tag="JD016_10395"
     CDS             complement(12273..12839)
                     /locus_tag="JD016_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAU5000045.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13000..13653
                     /locus_tag="JD016_10400"
     CDS             13000..13653
                     /locus_tag="JD016_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAU5000046.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13811..13987)
                     /locus_tag="JD016_10405"
     CDS             complement(13811..13987)
                     /locus_tag="JD016_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000047.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5473 a         1384 c         2375 g         4773 t
ORIGIN      
        1 ttttaaagaa attagtgtta ttatgaaaac ctaagttaat ttattaaata aataattgaa
       61 taaaaaaatt ttattatgta tttataaaat ttttgttaca aatttggcat aatatagtat
      121 actttaagtg tgtattaggt taaatctata acaaattaga cgtatgatat agacttagtg
      181 tttattggca tttacaagaa atttgttaat atcaactatt tgtagtttgg gcagttataa
      241 ttattatagg ggggatgtcc aatacaaaac aatcaaccaa gaactaatat tatattataa
      301 agagttatag ttttgtgtgt ttatgttttt aattttgtgt gtttatgttt ttaattaaat
      361 ttaaagtggg aaaaatatat ttgttagaat ggagaatagc gagatgagta ataaaatagt
      421 tagtaagaga caattaacag atagtattta tttaatggaa atagaggcac ctagagttgc
      481 aaaatcttct caaccaggtc aatttataat aattaaaaat gatgaaaagg gagagaggat
      541 tcctcttaca atagctgact atgatagaga aaaaggaaca gtaactattg tattccaaac
      601 agttggtgct tcaactaaaa agttagcaat gtttgaagaa aacgattttg taatggattt
      661 tgtagggcca ttaggacaag caagtgaatt tatacatgaa gatatagaag aattaagaaa
      721 taaaaagata ttatttgtag ctggtggagt tggttctgct ccagtttatc cacaagttaa
      781 atggtttaaa gagcatggat tagatgtaga tgtaataatt ggtgcaagaa ctaaagaatt
      841 aattatatta gaagatgata tgaaaaaagt agctaaaaat gtatatgtat caacagatga
      901 tggtacatat ggatttaatg gaagagttac agacctatta aaggatttag ttgataatca
      961 aggtaaaaaa tacgaccaag caatagtaat tggaccaatg ataatgatga aatttatgtg
     1021 ccaattaact aaagaactaa atatacctac tatagtaagt ttaaatacaa taatgataga
     1081 tggaacaggt atgtgtggtg gttgtagagt tagtgttgga aatgaaacta agtttgcatg
     1141 tgtagatggt cctgaatttg atggtcattt agttgatttt gatcaagcaa tgagaagaca
     1201 gtctatgtac aagactcaag agggaagagc tatgctaaaa cttgaagaag gcgatagtca
     1261 tcatcatagt aattgtggat gtggaggtaa caaataatgg atgctaaaaa agtaaaagta
     1321 ccagttagag aacaagaacc tgcagtgaga gcaacaaatt ttgatgaggt atgcttaggg
     1381 tataataaag aagaagctat ggcagaagct aatagatgtt tagcttgtaa aaaaccaaaa
     1441 tgtgtgggtg gatgtccagt aggaatcgac ataccagggt ttataacaaa aattaaagaa
     1501 gatgatatag aaggtgctgc taaagtaata gctaagagca gttcattacc agctgtttgt
     1561 ggtagagtat gcccacaaga aagtcaatgt gaaggtgtat gtatacttgg aataaaatca
     1621 gatgctgtat caataggtaa attggaaaga tttgtagcag attggtcaaa agaaaatgac
     1681 ataaatttat ctgatacaga gcctaagaaa aatcaaaaag tagcagtaat aggaagtggt
     1741 ccagcaggtc tagcttgtgc tggtgattta gctaaaaaag gctatgatgt aacaatcttt
     1801 gaagcaatgc atgagccagg tggagtttta acttacggaa tacctgagtt tagacttcca
     1861 aaacaagctg ttgttcaacc tgaaatagac aatataagaa aattaggtgt aaaaatagaa
     1921 actaatgtaa ttgttggtaa aacaattaca gtagatgaac ttatagaaga tgaaggattt
     1981 gaagctatat tcataggttc aggagctggt cttccaatgt ttatgaatat accaggggaa
     2041 aatgcaaatg gagtattttc tgcaaatgag tttttaacta gagtaaactt aatgaaggct
     2101 tatagagatg attatgatac acctattagt tctggtaaaa aagttgctgt agtaggtggt
     2161 ggaaatgttg ctatggacgc tgctagaact gcattaagat taggttcaga atcatatata
     2221 gtttatagaa gaagtgaaaa agagcttcca gcaagagctg aagaagttca tcatgcaaaa
     2281 gaagaaggca ttatatttaa tacattaaca aatcctaagg aaattttagt agatgaaaat
     2341 ggatatgtta aaggtatggt ttgtataaga atggaattag gagagccaga tgattctgga
     2401 agaagaagac ctatagaaat agaaggttct gaatttgtac ttgatgtaga cacagttata
     2461 atgtcacttg gaacaagccc aaatccacta atatcttcta caacaaagag tcttgatata
     2521 aataagaaga gatgcttaat aacagatgaa aatggtcaga cttctaaaga gggtgtattt
     2581 gcaggtggag atgctgttac tggagcagca acggtaatat ctgcaatggg tgctggtaaa
     2641 acagcagcag catcaataga tgaatattta aaagcaaaag ttaatgctta atttgtaaat
     2701 taaagagagg gtgtctcaaa atgaactttt tagttcatga ggcactcttt ttgtacaaaa
     2761 tcaaatatgt tttaatcatt tcaacaatga aaatttcatt cctgtagaaa gtctaacttt
     2821 taggtcttag tacaatctct attttgatgt agccactttt ttagatagtt taataattta
     2881 tcagttatta ttgtaatatt tgatactaag taacataact ttgtctaact atgataaaat
     2941 agttatacaa taaaggtctt gtaaggagtg aataatgtgt ggctaataaa atcagatttg
     3001 tcaaatctaa cattaaataa tagaaaaata aattgtgtaa ttaatggtat tttaatatga
     3061 aaaaaagaga aaaagtattt gatgggctag tttttaagca tatattaatc ttattaatac
     3121 tattagtttt gattgattta acaagtacca tcatatatgc aagtaactct atggaaatta
     3181 cttctagaaa tatgattgag gcatcaaaaa gagaacttga aaactattta gaagtaaaca
     3241 tatctttatt aaaagctctt agtcaagatg atagattttc taatggtgaa acatcactaa
     3301 ttgaaaaggg aaaattatta aggccatatc aaaaggaata taatcttttt atgattggta
     3361 taacagatac aaaaggaaat gcttcaagta catataggga aaatgttaac tcaataaaag
     3421 ataagcctag ttttgagaaa gcaattaaaa caaagcaagt agtagtttca gatattgaag
     3481 tcagcaatgt aacaggtaac aaagtattta tcatatatgt accaattgta aaaaataatg
     3541 agatgattgg gaccattttt gcttcttttt attttcaaga tgtaaataat ttaataagta
     3601 gaagcaattt tgatgattct attaaatttc taatgataga taaagattat actattattt
     3661 ctcatccaaa caaaaaatat gtaaatgata aaagtaagat gcttgattta gaagggaata
     3721 ttataggaac cactaaaagt gaaatcttaa aaaatataaa taaaaaatgt caaggtgatt
     3781 ttttatcttg ggataattgg agattatata atgtaaaata cacgaacgta aagtggacta
     3841 attggacttt agtatcaaaa tgtaatatat ttaagaactt taaaaatcta atagttaatt
     3901 ttatgataaa gttgtatttt tatatagtaa tatttatgat attgtggaaa ttaagtaatg
     3961 ctaaattgat agaacaactt aaaaaattgg cttattatga ttctttaagt ggaataaaaa
     4021 ataaagaaaa gtttagaaaa gattccatgt atattttaaa gaattattat caagataatt
     4081 tttacttagt acaattggat gtcaataaat ttaaatatat aaatgagatg tttggatatg
     4141 ctgaagggaa taagatatta attcatatat cacaggtttt aaataataat acaaataaat
     4201 atgaaatttg tgctaggatg gataatgacc attttatatt gttgattgct tgtaacactg
     4261 aagatgaact tttaaataga ttatctaaaa taaataaaga aatttgtaac ttaaatacta
     4321 ctaactcatc taagtataaa attgttatgt caagtggaat atataagatt actaaaaagg
     4381 atgatattaa aaagattgac ttgttaattg atagagcaaa tattgcagca aaaagtaaaa
     4441 aggaaaaata tgaacattca tattcatttt ttaatgaaga tacaagaaat cgattgtaca
     4501 aggaaaaacg attggaagat aatatgaata aagctttaga aaaaggtgaa tttatagtat
     4561 attatcaacc taagtatagt ttagatgatg taaatgaaat tgaaggagca gaagccttga
     4621 taagatggaa tagtccagaa tttgggttta tttcacctat agattttgta cctttatttg
     4681 aaaagaatgg ttttattgta aatattgata tgttcgtgtt tgaagaagta tgcaaaacat
     4741 taaacaagtg gataaataaa gggtatactc ctgtaccaat ctctgtaaat atgtctagag
     4801 ttcatttata tagggataat tttatagaaa atataactga tttgataagt aaatacaata
     4861 tatctcctga gtttattgaa cttgaactaa cagaaagtgt tgtgtttgat aatttaaaca
     4921 tattgattga tataatgaaa aagattaaag agataggatt ccttatatca atggatgatt
     4981 ttggttcggg atattcatct ttaaatctgt taaaagacct ttcttttgat attttaaaat
     5041 tagatagagg atttttaatc gaaacaacag atacaaaacg tggaaaaatt attatttcta
     5101 agattgtgga aatggcaaaa gcgattgata ttaaagtaat ctgtgagggc gtagaaactt
     5161 atgaacaagt agagttcttg aaagagatag gttgtgataa ggttcaagga tacttatttg
     5221 ctaaacctat ggtattagat gaatttgaaa agcacttaaa ctttaaattt gattaatcat
     5281 taataattat tagactatga gataccctaa caacttactt acaaatatgt aagttaattg
     5341 ttagggtatt tttatggtta aagtagtata ttcacgatat tatggtattg tagtaaaaat
     5401 aaaaaattat ggagaatata aaatgaacat attgagtatt agaaatatat caaaaacata
     5461 ttgtggaaat attccattta aagcattaga taaggttagt ctaaatatag aaaagggaga
     5521 atttgtgtct gttatgggac catctggaag tggtaaatct actcttttaa atataatatc
     5581 tacagttgat agacagagtg aaggtgaggt tgtattagat ggatatgatg taagtaaatt
     5641 gaaaggagaa aaacttgctg aatttagaag gaaacaactt ggttttgtat ttcaggattt
     5701 taatttgatt gacactttaa ctgttggcga aaatattatg ttgccactta cactagaggg
     5761 gtcaagtatc aaagatatga atatacaaac taaaagtata tctaaatttt taggaataga
     5821 taaaatttta gatagaaaaa cctatgaaat ttcaggaggt caagctcaaa ggtgtgctat
     5881 tgctagagct attataaata aacctgccat acttcttgca gatgagccaa ctggaaatct
     5941 tgattcaaaa tctactgatg atgttttgaa attatttact aggataaaca aagaacaaaa
     6001 ggtaacaaca cttatggtaa ctcatgaagc atatagtgca agttattcag atagagtaat
     6061 ttttataaaa gatggttgta tatatacaga aataaaaaaa tctgaaagca gtaattcttt
     6121 ctattctgat atactagctg ttttgtctca aataggaggt gtaagatagt ggattttaga
     6181 aagtttgctt ataataatat agttagaaat ttcagggcat atttagcata ttttttaagt
     6241 tctgtttttt ctataatgat attttttgtt tttgcagtgt ctatgttcca tccaattata
     6301 acaaaatctg gaatgcagag tggttctact gcatttatgg ctcttatgac ttcagaatta
     6361 ataatattcg gttttagtct actttttata ttgtactctt taggaaattt cttaaaatat
     6421 agattgaaag attttggagt attgatgata attggaatga gtaacagaca actgaaaaaa
     6481 ttgatattaa ttgaaaattt gataatagga tttttagcag tgatgttagg aatattactt
     6541 ggtttatcaa tttcaaaatt gtttttattg tatttaagca aactgtttta tatgaattta
     6601 actgaatttt attttccagt aaaagcaatg ctattgacta ttgtatcatt tatgattttg
     6661 tttttaataa caggaccaat gagcttaaaa ttattgagta aaaagagtat tttagagctt
     6721 ttagtaggta caaaaaagcc taaaaaagag ataaaatctt caaagctatt tggaataatt
     6781 ggtgtaatat gtcttatatt aggatatgct atgatactat ttggcaataa gttaaaaata
     6841 aatggaggta tgtatacaac agcactatta attactttgg ggatattttt atttttttct
     6901 caatttacta ttttgatatt aaagtattta aaaaataaag aaaaatttta taaaaacaaa
     6961 gtaaatatgt tacttataag taatttgata tataaattaa aggataatac acgattgtta
     7021 tttttaataa caatactttt atcggctact ttagtagcat ttacaacaac ttcaacactt
     7081 gtaagttctc aaggagaagc ttctaaaaat aatttcccaa tggtttactc atatttttca
     7141 gagcataata atgaaaaaga atatgaagaa ctacaacaaa ttagagaagc aatcaaagga
     7201 tatgattata aggagttaag ttttcctgta cttaaatata atagggaagt cttactaagt
     7261 gtaaagaatt ataacaaatt atcaaaggaa ttgggattag ataaaattag ttcaaagtta
     7321 gaaaaatatg aagggattat aattcctagt agcaattcgc aagaacatat aaattcttta
     7381 aaacaattaa aaaattatag tataaaaaat ttaaatatag aagtaaaaaa atcaaaaaat
     7441 aaaataattt ttcctacagg actgtttagt aaagttgtag ttataagtga tgacttatac
     7501 aatgaagtaa aaggtgaatt ttcaaaaatt aattttcatg gatttgatta taaaaactgg
     7561 caaagcagtg cgaagataac tgaaaattta aaagaaaaac attttaattt ggatagagaa
     7621 tataacaggt catatttttt aacattacca gatatgtatt tagtagagtt acaacaaagt
     7681 aaaattcttc aatttatggg tgtttttata ggagtgatat tatttatagc agtttttagt
     7741 tttttatact tcagattata tacagacgag atagaagata atgtaaagta caaaaaacta
     7801 tcaaaaatag gtctatcttt taaaaatatg aataagattg ttagtataga gataggtaca
     7861 ttgtttttta taccgtattt tatagcaatt ataaatagca taatttcttt actgttttta
     7921 aatcaagtat taaataatag ttttaagtta gaaaatttgg gtatattact actgatatca
     7981 tctatatatt cactttattt ttacttatta aaaaaaattt atactagaaa agtttggttt
     8041 tttgattaaa gtggtatata atagatatat aaattaatat ttaaaaagta gttaatatta
     8101 ggggagggca tttgcacctc ccttgtaata tgtggaaaat tgaatattag aagaaggagg
     8161 agacttgatg gaaaatcaac aattattagg aacagaacga attagtaaat tactactaaa
     8221 atattctata ccagctataa taggtatgtt ggtaaatagt ttatataatg tagtagatag
     8281 aatttttatt ggaaatatac caggggttgg accacttgca ataacaggtc ttggtgtaac
     8341 aatgccaata atgacaataa tacttgcatt tgggatgttg ataggtatag gaactactac
     8401 taccatatca ataaaacttg ggcaaggtaa agtagaagag gcaagaaaac ttataggaaa
     8461 tgcaatgacg ttatctgtaa taacaggaat tatcataatg atacttggaa tattatttgc
     8521 aaataaaata cttacattgt ttggagcaag tgaaaatact ttaatatatg caaaatcata
     8581 tatcaatatt atattattag gtacagtagt aaatcttctt tccttttctt taaaccactc
     8641 aataagagct gatggtagtc ctaaaatttc agcaggaata atgatagtag gatgtttaac
     8701 taatatagtt ttagactgga tattaatatt tggattcaat ttgggaatac aaggagctgc
     8761 aattgctaca gtaacttctc aggctcttac agctatttta actatagggt attatataag
     8821 tggaaaatca aacttacgat tcagtaaatc taatctaaaa ttagataaaa agctaataaa
     8881 agcagtgttt gcaataggta tgtcgccatt tgcgatgcaa cttgcagcaa gtttagttca
     8941 agtaatatca aatattgcac ttaaaactca tggtggagat ttagctatag gggctatggc
     9001 tactatttct tctattgcaa tggtattttt gatgcctatc tttggaataa atcaaggagc
     9061 gcaacctatt ataggtttca attatggagc tgaaaaatat gatagggtta aaaaagcata
     9121 tttaggttca ttagtagtag ctactataat tttatgtatg ggtatggtag tagttatgct
     9181 tttcccagaa gctattatag gtatatttaa taaagaccct gaacttatga atatatctgt
     9241 taatggatta agaatatact tacttatgtt gccaattgta ggcttatcag ttacaggaac
     9301 aaactttata caatctatag gaaaagcaaa aatggctatg ttattgagcc ttttaagaca
     9361 agtaatttta ttgataccag cagtattaat tcttcctaca ttcttaggtt tacagggtgt
     9421 atggacagct caaccagtgt ctgattttat agctacagta attacaggta tagttgtatt
     9481 tagagaatta aaaagatata ctcctaaaac tgaaaaatta aatgaaaatg aaagactaaa
     9541 cgagataact actgagtaaa aatataaatt cagataattg agcaagaaat tgcaaaaaat
     9601 acgattatat gataaaataa aaccgctgtc aaggataaga gaaattctaa ttatggagtg
     9661 attttatgga atttacaaag aaaagcaaat taaaaaatat gtatgtaaac atgaatgtag
     9721 ctagaatgtt gtctaagata aatgagtata agggtagaca actgctttac aaaaaacaac
     9781 caaaagaaat tttagaaaac ttagaaaaaa aatctttagt agattgttct gaatctacat
     9841 acattggaaa tcaaagagat agtagtaatt ttaatctaga aaagttgata agtaatgaag
     9901 ttacacctag aagtagagag gaattaagta ttgttgaata cagagatgtg gtaaaaacta
     9961 ttaacagtgc ttatgagagt attcccatca gttcacaaac gattcttgag cttcatggat
    10021 acttatataa attttcatct actagaggag gaagttataa gtcagataat gattttattg
    10081 aacacaattt aaaacctagt gaatttataa gtattgattc aagtgtcaat aaagttgaaa
    10141 aagctgtaga agaaatatgt gaagcttata atacattgat agaggaagat gaaattgata
    10201 tattaatatt aatttctgca tttgtgttag atttcatatt gatacatcca ttcaaagaag
    10261 gtaatataaa aatggcgaga gtacttatcc tattgttact aaataaaaat gggtatgaag
    10321 ttggaagata tataagttta ggaaagatat ttgatgatag ttcatatgaa tattatagta
    10381 atttaaatta tttgaaagcc tcaataggta gtgaaaaagc agatatgaat gcatggattg
    10441 agtatttttt agagacgata ttaactgcat atgagaagct ggatgatagt ttaaatatat
    10501 ctgataagaa aagacaaaca aaaactagta gaatagagaa aataataaat tctacccttg
    10561 gatattttac aaaagaagat ataagagatt tatgtcctga cattccagaa cctactataa
    10621 atagagtttt taataatcta agaaaacaag ataagataga agttgttgca agaggaagaa
    10681 gtgcaaaatg gaagaaaaaa tattaaaaaa tgtttagata actaaaatgg tatagtgttt
    10741 tacactatac catttttaat gtcattagta tgagcaataa tttaacaact taagcaatat
    10801 caattttatt tattgattat ttttgaactg aaaaatggcg ttatatggta tatctcagtg
    10861 gtaattaatt ttggatgaga agatataata ttacagaata tattaggagt atatgtagaa
    10921 aaaatataat aaaagtgtga ggcttagtaa ttaacaaatt atgtggcttc acactttttt
    10981 ataaaataaa gatacttagc agaataatat tttagttgat atgtttataa gatggtactt
    11041 gtttgggtaa aagtataaat agtaaaaaag tagaagtaat gtgtataaaa acataagtta
    11101 cattctgtaa taatagttat tttataattc aacaaaagaa tagaatgggt atatttgtga
    11161 aataataata taaaataatc ttaggagtga gcttatgaag aaaaaattat tatatataaa
    11221 tgtaaattca aaacctgaag acttatcttc aagtaaaact gtagcaagaa aatttataaa
    11281 taaatttatg gaaaaaaata aagcttttga ggttgaagag atagaccttt ataaagaaca
    11341 tataccaaga cttgagtatc agtactttga aaaaagaaat agcatagtta gtgaagaaaa
    11401 tgctaaaaat ttagacgata aggatagaaa agagcttaca aagattagaa atctttgtga
    11461 ccaatttgtg agtgcaagtg tatatgtaat agcagcacct atgtggagtt tatcttttcc
    11521 agcaccactt aaagagtata tagattgtat aattcaagat ggaaaaacta tatcttttga
    11581 aggcaatgat aaaccacaag gaattttaaa tgatattgat agaagtatgg tatatataca
    11641 gtcttctggt ggacacatac catgggtgtt gaagccagtt atgaataaag ggttaaatta
    11701 tgtggaaagt attatgaaat ttataggaat taaaaaattt gatgaattgt tagtagatgg
    11761 aactggaact agtgaagaag aaagacaggc tgcaatagaa aaagcatctg aaaaaataga
    11821 tggtataata gatgggatga aattttaatt ataatatttt attaaaaata taaaggaaaa
    11881 acctctttta atgtagaaac ttatattgaa ggaggcgaga atcatgcgag aagaaaaaag
    11941 taatgaaaag tatgattgtt attggtgtaa tcaagagaat aatttctgtg tagaaataaa
    12001 agataatata gtcatgatag atgatggcac tggtacgtta aaacaagccg ttttcatagg
    12061 gtataaacaa atccaaatta atttaaattg ttcacattgt caaaacttaa atagaataaa
    12121 attaaatttg tagaatttta aaatggaaat tattgaagga attaaaaata gaaagtgtta
    12181 atatttagac tgaaaattat attttataca ataaaagttt atttaaataa cattgtattt
    12241 taatatttat tttcagtctt actttattaa aattattttt tggttatgtc tggaaaatat
    12301 ctgtctacta atttttctga aatatttttt aattggtcag ccattgaagt cagacttaat
    12361 aaggctatca ataaatcatc tttattttct ggatttatac taatattttc aaaattagac
    12421 tctataaagt ccggtatatt ctttctttga ttttctttta ttaacatgaa tttttcgtaa
    12481 catgaaacta aatctttttc actgaaatta tccttttcat acacaccatg taagattctt
    12541 ttaatttctt gtattgtaag tgtatttttc atagaataaa taattatcat ctgtaaaatt
    12601 tgttctttag tatacttttt tcccttaact ggtttgatta atccctcttt gctgtaatta
    12661 tttatcatgg ttttagtaag aattttatca ctttcaaatc ttttattagc agaaagctta
    12721 ttgtcaatta gagaaataat ttgatccata tataagtcaa tagatggaat atcgtttgaa
    12781 gttatatctg cattatttag tgtttctagt attatagtat taagttcatt attcatcata
    12841 tgttacctcc ataatatata taatacacta aattagaaag taaaacaata ttaaggcttg
    12901 taaaaaaaac ataaagtggt ataattaaat atataatata gtaatgaaaa ctatatttat
    12961 gaatctgatt tttaaaatac ataattgtag gaggacgaaa tgtatcaatt ttttttaaag
    13021 ggtagagacc caataagcag tttaactcat tttataggtg cttgtttatc tcttttagca
    13081 actataattt tagtttttca atctgttaca ttacaggaaa cttctctctt gatgatagta
    13141 tctgtatcgg tatttggact ttcactaatt gctttatata gtgcaagttc gtattatcat
    13201 tttttaaagg ggacaccaga acaagaattg ttttttagaa aagtagacca tgcaatgatt
    13261 tacgtattga ttgctggctc ttatacacca atatgtctga attttatgga aaaaaaagaa
    13321 ggcataatat ttgttacagc aatatggata gttgcattta ttggtataat aataaaaata
    13381 ttttggatgg atgcacctag atggttatct accagtatat atttacttat gggatgggca
    13441 attgtttttg atataaatgc attcaattca attcctaagg attgccttag actattaatt
    13501 atggaaggtg tatcttactc tataggtgca attatttata taattaaaaa gcctaatata
    13561 agtcctgagt ttggatttca tgaaatattc catattttta ttatgatagg ttctttattt
    13621 cactttttag cagttttatt gtatgtttta tagttttaaa agatttgatt aattgatact
    13681 aaaattctac aatgaataaa atggaactgc cttatataga ttatatcttc gccaataaga
    13741 cagttcctta ttataaatat taaattctac ttataataaa attttgataa aaatagtcca
    13801 ttcaaaatgc ctattccata attcctttaa tgtacttttc atttacgaca tatagtttga
    13861 atttatgtac tataattata tcctctaaat tctctgaaac actaacaatt ctgacacatt
    13921 tgtttctaat ttctacaggt aaatctaaat catttgttaa attcacatag tcatcatatt
    13981 tatacataaa atcatccctt tacat
//
LOCUS       DACXMZ010000089        13903 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.090, whole genome shotgun sequence.
ACCESSION   DACXMZ010000089 DACXMZ010000000
VERSION     DACXMZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13903
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.090"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            102..332
                     /locus_tag="JD016_10410"
     CDS             102..332
                     /locus_tag="JD016_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000048.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            389..1039
                     /locus_tag="JD016_10415"
     CDS             389..1039
                     /locus_tag="JD016_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAU5000049.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            1242..1913
                     /locus_tag="JD016_10420"
     CDS             1242..1913
                     /locus_tag="JD016_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000050.1"
                     /translation="MAKILVVEDEKRMQNIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHYIVTLIRSGYK
                     FEVKE"
     gene            1910..3040
                     /locus_tag="JD016_10425"
     CDS             1910..3040
                     /locus_tag="JD016_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU5000051.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3472..4317
                     /locus_tag="JD016_10430"
     CDS             3472..4317
                     /locus_tag="JD016_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000052.1"
                     /translation="MIKVRELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4317..5345
                     /locus_tag="JD016_10435"
     CDS             4317..5345
                     /locus_tag="JD016_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000053.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5348..6361
                     /locus_tag="JD016_10440"
     CDS             5348..6361
                     /locus_tag="JD016_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000054.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(6850..8100)
                     /gene="cme"
                     /locus_tag="JD016_10445"
     CDS             complement(6850..8100)
                     /gene="cme"
                     /locus_tag="JD016_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HAU5000055.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8186..8932)
                     /locus_tag="JD016_10450"
     CDS             complement(8186..8932)
                     /locus_tag="JD016_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAU5000056.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(9066..10028)
                     /locus_tag="JD016_10455"
     CDS             complement(9066..10028)
                     /locus_tag="JD016_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAU5000057.1"
                     /translation="MSSEKLSQLEPLIISQYSNINGIIIARKGKVVFEKYYNGYNADD
                     IFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPEYTCSPSEICKKAVTIRHLLT
                     MTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIGTFKYSSAGTHLLSAIITKTT
                     GKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKNLKGWAKDRQGYSIGSWGLTL
                     SARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSNEYGYLWWLCKKDGVLWYSAM
                     GTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYILTSIID"
     gene            complement(10088..12508)
                     /locus_tag="JD016_10460"
     CDS             complement(10088..12508)
                     /locus_tag="JD016_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAU5000058.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHNDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQTNSSESEIGDEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHNILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12594..13514)
                     /locus_tag="JD016_10465"
     CDS             complement(12594..13514)
                     /locus_tag="JD016_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAU5000059.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         4911 a         1806 c         2188 g         4998 t
ORIGIN      
        1 gttttctata ctataattat ttaaaactaa atataaatgt tggaattatt taggacttga
       61 aattgtctaa aaataaatat aagtgatgaa gaggtgatgt gatgagatta gatatggata
      121 gtatgttata tgtactaatt tatatgacat tattggtatc tatcattgga agtatatttt
      181 atttttgtaa agacctctta aatcataaaa ttaagtatgt tttaataaga gacatatttg
      241 taaatagaat tacatattct gaagaacggt tgcctaaaca gtatatagtt tttcagaaat
      301 atgatatttg gaggtattgt agtttgtttt aatttgaaat gaatctatta aaaattctaa
      361 agtttaaaaa taaaaaggag agaataaaat ggatttcatt tcaagtatat tgcaaggatt
      421 attaagtata ttatttaatt ttacaggtga ttttggaatt tctattgtac ttataacttt
      481 aatcgttaaa ttagtactta tgccactatc attaaaacaa agattttctg taaaaaaaca
      541 gcaagaatta gctgaaaaaa tggaacatat aaaagaaaag tataagaatg atactaaaga
      601 attggaaaag caaatacaag taagttctgt agaaagtatg aagagtatgc tgggatgtct
      661 tatagttttg gttcaaatgc cagtagtata tgctttatat catgtatgtt tagatatgac
      721 aaaggaattt acaacaatta taattccatg gattgctaat ttaggtacat ttgataattt
      781 gtttatatta ccttgtatat atacattaat tatgttagct tcaaatttag tgaattatat
      841 accatattta aaaataaatt ctcaagtaaa gtttaacaaa caaatggctg tggtgactac
      901 cataacaagt cttatactaa ctgttagaac tccagtagcc ataggtctgt actttataac
      961 aagtgcaatt tattcattaa ttgaagatat ttgttttaga atatatttta atcgaaaaaa
     1021 tagaatagtg acaaaataat ataattatta ttagaaatat tttagcctgc taacatagaa
     1081 aaagttagta ggctattttt atctatgata tcaattccat taagaatagt ctaaaaaata
     1141 ctatcaaaat gctataaaaa atactataaa aaaatagttg aaaaataaat aaattaatat
     1201 aaactaaaat aaagcctaca aaaaagaggt gataaataga aatggctaaa atactagttg
     1261 tagaagatga aaaaagaatg caaaatatta ttgtagaata tatgcaaaag ggaggatata
     1321 cttgtataac tgctgatgat ggagtcgagg cacttacaat attaaagagc aacaatatag
     1381 atttaatgat attggatatt atgatgccgt atttagatgg attttcagtg tgtagagttt
     1441 cacgtgaaat gactaatata cctattatta tattgacagc taaaggtgaa gaggaggaca
     1501 aattaaaggg ttatgaatat ggcgcagatg attatataac aaaaccattt agccccaaag
     1561 tacttttagc aaaagttaat gccttgttac gtagatacac tactgatatt cccaaaaatt
     1621 cattgagttt aggaaagatt tttattatgg tagcatcaag acaagtgtat gtagaagata
     1681 aattgattga tttaacatat aaagaatttg aattattgcg tttatttatg gaaaacccta
     1741 atcaagtttt ttccagagag aaacttttaa attgtatatg gggatatgat tttgaaggaa
     1801 acacacgaac tgtagataca catataaaaa ccttacgcaa aaagcttggt agtgaaggac
     1861 actatatagt tacattaatt cgttcaggat ataagtttga ggttaaggaa tgaatagttt
     1921 aagaaagatt atagaaaaac tgaaaaatgt agatatttca aattggatta aacaaaattt
     1981 tggactatat tcagttagaa aaaaagtgtt tttactttct aaattatcag gagcgttaat
     2041 tatattattt tatctggttg ttgaagaatt ttcgataaac tctaaatttg aattttggat
     2101 ttggtttata atactggtta tatttattat gagtattgat ttcttattgg gaaaatttat
     2161 ttcagaacca atcacatcta taaataaatc tgcaaaaagt atgtcccaac ttgatttttc
     2221 taatccatgt actgtaaata ctaatgatga atttggagaa ctttctagga gcctgaatac
     2281 aatgtctaca aatctacaac aggcattatc agacctagaa agtgcaaata ttcaacttga
     2341 aaaggatgta aataaggaaa gaatgctatt ggaacaaaga aaggagttgg tagatacaat
     2401 ttcacatgaa atgaagactc cacttggcat tatacgtgcg tatacagagg ggcttatgga
     2461 tgaagttgat gaagaaaaaa agaaaaatta tatgaatgtt ataatagaag aaactgatag
     2521 aatgaacaat atgattgtat cattattaga cttatctgca ttggaagcag gagtttctaa
     2581 acttaaccca gagcgttttg attttataga gtttgttgaa actgtggcag gtcgtctttt
     2641 aattgatata ccagatgcca atttttattt tacatatgat ttgccagagt atgaagtttt
     2701 tgtagtggca gataaaatga gaatggaaca agtagtagag aatctagtta ttaatgcaaa
     2761 gaagcatgtt gtatataatg gtaatttaga tttatcgatt acatgtgaaa atggcttgct
     2821 tttatttgaa atttataatg atggtaggtt tatagagcaa gatgagattt caaagatttg
     2881 gtctaagttt tatcgtagtg ttcaatctca gcgtacagga ggttcaggtc tagggcttgc
     2941 aattgtttca caaatactta caatgcaagg gttagaatat ggagtgaata atcgtgatag
     3001 aggagtagag ttttatttta tgattccaat tgatgaataa tttagtatta atttataaat
     3061 cataagtatc tgatgtaaaa tatttttatt tgtatatgta agcatttaaa gagaataaaa
     3121 taaaaaaact taatgaaaaa agcttaatga aaaagtctaa taaaaagttt aataaaaagc
     3181 ttaataaaaa gtcttgtttt cctgatgaaa acaagatttt ttattaagct ttttattatt
     3241 tcacttcgat ttcacaagag agacactctg atttcaccaa atagaaataa aataggttaa
     3301 ataaattaaa aaatagaagc gtaattaaat aaggttacaa gggggagtac ttatgaaatg
     3361 gtatacatgg ttgactatta tattgataat attgctttct attctgctta aaattaaatt
     3421 tataaaatgg tgggatagac gacaaaaaaa tgataaagga ggttatgata gatgataaaa
     3481 gtaagagagt taaaattttc ttatggaaaa gataaacaga tattgcatgg acttaatttt
     3541 gaagtaaaag aaggagaaat ctttggattt cttggaccta atggttcagg aaaatccaca
     3601 acacagaaaa tcttaaatgg cgttttaaaa ggctacaatg ggcacgtatc catttttggg
     3661 aaggaagtta aagcatatac agagtctcta tatcaaaaga ttggtgtttt atttgaattt
     3721 ccttatttat ataccaattt aagtgcaatt gataatttag aatatttttc gtctttttat
     3781 cctaaaaatc aaaggagaga catatatgaa ctcttagatt tgttagagtt taagaaagag
     3841 tttataaata agcctgtatc atcctactca aaaggtatga agcaacgtat tagcatggca
     3901 agagcattgg taagtaatcc aaagttgtta tttttagatg agcctactag tggacttgac
     3961 ccatctggag ctgtactatt tcgtaagatt attgaagaag aacgaaaaaa aggaacaact
     4021 atctttttga ctactcataa tatgctagat gcagatttaa tgtgtaacaa tgtggctttt
     4081 attgcagatg gaaagattat ggtaatagat aaacccaaaa atttaaaaat gaaaaatagc
     4141 aataataaag ttgaagtgga atttgtatat aatggaaatc gagatattga aattgtagat
     4201 atacaagagt tagaatctgg aataactttt aaatatgatg aaatcttaag tgtacattca
     4261 aaagaaccta cactagaaga agtttttatt aaatacacag gaaggatgtt agtgtaatgt
     4321 attctaaatt ttttacctta gtaaaaaaag atttttggat gatgatttat ggtaaatttt
     4381 ttgtgctgac acttggctct ctcatacttt attcgtgtta tattaatttt ggttatgtaa
     4441 agtttatgga tgaagatagt tatcacaata atgtatatct atataatcca gagaatataa
     4501 gcatgagtgc atctccatta attaatcaag tttcttctat aggtgaatta aaagcaaaac
     4561 ttgatgaaga tacaagtgca gtttgtattg atatgagttc cagtaagccg aaagtaattt
     4621 tacatgaaag tattaaagga gttgacaatt atagagctga ctatgcattg tcattattat
     4681 catcagaaaa agaccataat agcaaaatat cagacaacaa tgcaataatt caaattaatg
     4741 ataaggatac atctgagata aaacaaagaa aagacatgac ttgtgagatt ttattctttg
     4801 aaattgtagc tattggattt ttagggattg cctctttatt atttaaagaa aagcagatgg
     4861 gagtaatgcg tattactggt attttacctg taaaaagaag tctttttata ttatcaaaga
     4921 tatttgcttt ttttatatgt gacctatgtt ttacagttct tttagtagta tttaatattg
     4981 gatttttaga aggaataaaa atacttccac aagtattagt tcaagctagt attttatcaa
     5041 ttattatgtc tttgattgga ataggatgct caatgttatt gagagatttt aagcaatttt
     5101 ctcttatata tcttgtggtt gctgtattca taactacacc tatatttcta tctgcaaata
     5161 catcttttga aatagattgg attaaatatc atcccttcta tcatgtttat agtcagctta
     5221 aaaatgcgtt tttcatgaat ttttcaaaag aattagttta ttttgtagtg tgttctttga
     5281 caataatagt tctattttta cttgtaaaat atatttttaa taaagagttg gttagggagg
     5341 gataatcttg ataggattac gttaccagtt aaagagtata aaaaaagata aaatgtgtat
     5401 aatttccttc tttttaccag ttgttatggc gattttaata aattttatgg gaacaattga
     5461 tatatcttca ttaggagaga tacgttttgg tatagtgaag gatacacttc ctgtacaaac
     5521 agagcagtgg ttgaagaagt atggtatggt aagtgtatat gacacaaaaa tgcatttaat
     5581 acgtgaaatt aaggatatta aaacggacac aattggagtt gttagagatg gtaaagggat
     5641 aaaaactgtg ctttatggaa atgagattaa tgctataaaa aatgttggaa agacattgcc
     5701 agagatgttt agactaaagg gtgatttaaa agatgttgag gttacaatat tgcctaaaga
     5761 tagtatttta aaagagtttc aaaatgtttt tattgctatg attttaatta ctgctatgtt
     5821 tatgggatgc accttcaatg caatgaatat tatatctgaa aaagaagatg gaatatcatt
     5881 tataaatgaa gttttgccac agagtagaag tcagtatatt atacaaaaag tatttttggg
     5941 tcttttatgt gggtgtttat cttcaatgat aactgtattt atatgtttgg atatttcaat
     6001 attagatatg ataatgatgt tgttactcgt tgtattttca gcatttattt catcacttat
     6061 aggtttattt attggaaagt tgtcagaagg tcttatggta ggaattgttt atattaaaat
     6121 agtaatgatt ttatttatag gtactccttt gttaatatat cttctagggg ttaataccag
     6181 tggtataggg aatttatgct atataatgcc ttcagttgct acatttaatg gtattatgga
     6241 gattattaat ggtaatgtca ttattataaa agatattgta attttggctg tacattgtgt
     6301 agtatggttt atgatatata ttattttaga taaaaaaaga aagttatctt ttagtagata
     6361 actcagtata attataattg ggacaattta ataggtgatt taatatactt tgtatagtgt
     6421 aatttatgta gtgcaatagt tagttttatt ttataattat tcaatataat ttagtaagaa
     6481 gttcagtaca attagagcta cttagtggaa aacttaatag gtacttaaac tatataaata
     6541 ctttattttc ctccatttgt actataaatt taatttatga tataaatgga ggtattatta
     6601 tatgatttga taaatttcat cattataacc tacaagtaaa ggtattatta tataaagtta
     6661 ataaagatag tatcaaaata ttatttgcta taaagagagg gactcaaaat aaagataaac
     6721 ctatttttca atgttgaaat gataaaatat atttgatttc atataaaaaa ttttcttcat
     6781 aaattaaaaa gtccatttta agccccctca tttttaacca gataaatcga gttaaactat
     6841 ttaaatatat tactcggatt catttgactt agatattcct tgcttgtaaa attgttttga
     6901 ataggggata gccaaagcaa aaaataaaag tataatccct gctatgatta ttaaattatt
     6961 aatagaaatt acatctgcca ttggtccgaa aatcatcata ccaagaggaa taaaaccagt
     7021 atatataata ataggtaagc taagaactcg acctagcatt tgtggtgcta catgctcttg
     7081 caacatagtc atagctgatg cttgaatgat tggaattgaa aaattcgtaa aaaacataaa
     7141 tataaaaaat tgccaagctt gtgttacaag acctagaata attgaaaaaa tagcatagga
     7201 taatattgct agaaaaaagg tcttatttct atttttaaaa ccaccaaaaa ctcctataat
     7261 caatccacct aaaaccatac ctaaaaatcc aatcatttca ataatagaca ggtatatata
     7321 acccttgcca aatgttcgtt caataagaag tcctataaga aatcctgatg gaacacaaag
     7381 aagcatgaat gctccataag taaaaagcac ttttctaatg aacttatctg aaaaacagta
     7441 ctttatccct gataccatat ctgcaaaaaa tgagactttt tcagtttgtt ttggtatatc
     7501 atgtttagga attttgatga atatcaataa tacaatagca ataattgcag taaatacatc
     7561 aattaaaaga acttgattta ttttattacc aaatccaagt attgcagcag ctacaatagg
     7621 aactataaat tgtactatag attgtaggct actatttata ccattaaaac gcattaaatg
     7681 ttcttctggt acaagttgag gaatcattgc gtttacagca ggtgtttgta caccacttcc
     7741 tagtgaacgt ataacagata ttactgatat ggctattaat tcacttgttg tgccatttga
     7801 tgacatcata aatagaatta gaactaatgt agatatagca attgttgaat ctgatacaat
     7861 tatgagtacc tttcgattgt agtagtctgc ccatacacca gcaaaaaaag aaattatcat
     7921 ttgaggtata aaggagcata cagttaaaac tgttacccat acacctgatg atgtctgaag
     7981 tgtgacatac catatcattg ccatttgtac aagtgatgag ccaaataagg aaatatattg
     8041 acttgctaaa aaaagaatga cttttttctt ccatgtagaa ttagtatttt tgttttccat
     8101 cctattcctc ctttgatttg tgtgttgaat ttttcttgct cttaaggata acacacaaat
     8161 caaagtctgg agttagagta gagggctagg aaaattgttt tttcataatt tgaagatgag
     8221 atttcatggt ttttgcatta tgttcagctt ctttcaatga agttatcagt ttgtcacaca
     8281 ctgagataat ttcgtcatca tcttttggag tgtcaaggac agttttaata ttagtattag
     8341 gattagaaga taaagcattt aacattcgta aaatagctga aagagaatag tttgcacaac
     8401 gaagtgcgct tataatttta agtcgtttta tatcttcatc agtatataca cgataaccat
     8461 tttgttttct ttttactttt aacaaaccat tcatttccca atttcttaaa gtatctattg
     8521 taacatttaa gtgttctgca gtttcttttc tagttaaaaa gagtttattt gtttctggag
     8581 tatttccatt taaaatctgt gcaacaatag taagtgcttc ctcggcatta gatttttcta
     8641 tttctaattg ttgcaagtat tgatttacaa gactaatagc cttatcaaag tccctattcg
     8701 ctgatgttct tacaatctca agggcatctt ttcttaatcc cctttgtacc acttcaattt
     8761 tcaaggctgt acgagcaaat ctaaattgtt ctatatgaaa atcattaaag atacgataac
     8821 cattggtatt acgcttaggt ttaggtatta agtttagttc ttcatataaa cgtactgtat
     8881 taggatgtac accaattaaa tctgctacct ctgatgtcgt gtaagtgtcc attagtattc
     8941 aactcctttt agatatatag tattagtcat aataacacca aacaaaagtc tggtgttata
     9001 atggagggtt tggttatcta agagtaatta gtttatgaaa gagtaattaa tttatgaaag
     9061 agtaattaat ctattataga agtaagtata tatttttcaa taaactccca tctatcacga
     9121 ggtttaaaca ctattttaga tggtatggct attactaagt catattgtgg aatacaacaa
     9181 atagcatttc ctccagttcc cattgctgaa taccacaaaa caccatcttt tttacatagc
     9241 caccacagat aaccgtattc attactattc attttaactg attcttcaat ccacgattta
     9301 gatataatac gtttattatt ccaaatacca tcatttaaat acagaaaacc aaatcgcaac
     9361 atatctctag cactcaatgt aagtccccag ctacctatag aataaccctg tctatctttt
     9421 gcccaaccct taagattttt tccaaagaaa tcttcaaaac caaaatcttt catatcataa
     9481 tctggtataa ctttcatacc aataggttta aataagtatt cattagcaaa ttcacgagca
     9541 gattttccag tagtttttgt tataatagct gaaagtaggt gcgttccagc tgaagaatat
     9601 ttgaaagtac caatttcgcc tttttgacca agttgattca aggtatattt tatccaatct
     9661 ttttgtttgc aaagtctttc taaagactca gtcatatttt taaaagggta tggtgcagtc
     9721 attgtaagta ggtgacgtat agttactgct tttttacata tttcagaagg actacaagta
     9781 tattcaggga aaaaatcaac aaccttttgg tcaacatctt ttatatatcc tttatctatt
     9841 gcaataccta ttaaggctga tataatactt tttgtaactg atgcaacatg aaaaatatca
     9901 tcagcattat atccattata gtacttttca aagacaactt ttccttttct tgcaataatt
     9961 attccattta tattactata ttgtgagatt attaaaggtt caagttgtga gagcttttct
    10021 gaactcatac caacacttga aggatttact ttttcaaata ttaaatctaa gcaattgttt
    10081 ttattcatta aagatacctc cctgttatag attttggatt ttcttttaga gtttttggag
    10141 aaccttctgc aattatataa cctcctttgt caccaccttc tggaccaagc tcaacaatcc
    10201 agtcacaaac atttaataca tctatatcat gttcaacaac aattacagaa tttccatttt
    10261 caaccaattc atctaataat tgaatcaact tagaagtatc gtatagactt agaccagtag
    10321 ttggctcatc caatacataa aggatattac catttcgttt tttaccaatt tcttttgcaa
    10381 gtttgattct ttgggattca ccaccactta aagttggtgt aggttggcct aattttatat
    10441 accccatacc tatttgttct aatacattta agtgtgaaac aatatttgga atatccttga
    10501 agaaatgtac agattcagaa acactcatat ttaaaatatt atgtatattt ttatttttat
    10561 atttaataga taaagattca tcattaaatc tttttccctg acattcactg catatatggt
    10621 ctatacttag attatttccc aaccagacct tttcatatcc actaccacta catttaggac
    10681 aagcaccttt ggagttaaat gagaaatgac cagctgtaaa tcctcgttct aacgattctg
    10741 gctgacctgc aaatagtgta cgtatcttat cccatatacc aatataagaa gcaggagttg
    10801 agttcatatt tttgccaata ggagattggg atatctctga atatccagaa atatgtttag
    10861 taccaatcag tctatctgca atagtttcaa caactgcata atcgtcttca tcacctattt
    10921 cgctctctga agaatttgtt tgattattga tagtatggtt attaaattgg cttctaagaa
    10981 gaggaagtag ggtgtctgat ataagtgaac tcttgccact acctgatttt ccagatattc
    11041 caaccatagc acccaaaggt aatgatacag ttacattttt aagattattt gtatttgcat
    11101 tttgaatagt tatacaaggt attttatcat tatgtgtaat gtttttattg cttgttctat
    11161 taggcattgg atattttcca gataaatact gcccagtaag tgatttgtcg gattgaagta
    11221 gaccttctaa atctccttga tatactaatt gaccaccttc aacaccagct tttggaccaa
    11281 tatcaataat atgttctgcc atctcaattg tgtttttatc atgctcaact actatgaccg
    11341 tattgcctaa atctttaagt ttctttattg attttaagat gtctattttt tctgattcat
    11401 gcaatccagc tgttggttca tcaagcacat aaattaatga gtccatctct gaatcgagat
    11461 gtgaatttaa aaatagtctt tgcatttccc caccactcaa agttgacatt tctcggtata
    11521 aggatagatg acctaatcgt gattctatta aatgttgagt tttggataaa atatcattta
    11581 caagatttgt accaaatgtg tttaaattac cttgatttat taagagttta aggaaatcat
    11641 caatttctaa aatcgtcatt tttccaagtt ctccaatatg ttttccgttt aaaagtactc
    11701 tacgtgcttc ttcaccaatt ttaaaaccat tgcaatctga acaggcagtc atagtataaa
    11761 ctccatttaa atcttcgccc cttttgtaac gattgtgaaa tacctttgat aaagaggaac
    11821 tttttttacc attttcataa tgaccataga gtacttcatt tcttatttct tcaggtagtt
    11881 gtaaaaattg tatgtctaaa taatcgttga attttttaga aaatactctc aaatatcctg
    11941 gtgtaatctt tgctctatct aagacttgtt ttagggtgtc atgcttatca aggactaatt
    12001 tttcaatatt aatctcaaaa tatgcaccat gtccagagca cttcatacac attccatctg
    12061 aagagttgta tgagaaataa cttggtctta atcgttcttc ctcatatcca caattattgc
    12121 aaattagatt atcgcttaca ggagtatcac acatagtaca cattatttga ccttccactg
    12181 aatacaatag tgtaagcatg ttgagaatat tggttcttgt gcctactgtt gaacgaggat
    12241 tgctctggcg aataatattt tgttgaacag ctatagtggg agctaaacct tgaatattat
    12301 caaacttatc ttcactatca atacctgcaa gtatacctaa agattgcaga tattgtttac
    12361 gcccttcctc aaatacaatg tcaaacataa ggctagactt acctgagcca ctgacacctg
    12421 ttgcaacaac aagcttatct ttggggatag aaacatctat atttttaaga ttgtgtatct
    12481 ttgctccatt aatttttatt tctttcattg gttcacctca ttttatcaat tttatcaaac
    12541 cgtaaaatag ggatatatta atccttaata caagaataga ttaggtgagg ttattaaaaa
    12601 ggttctatag gtaaatatat atcgaccaag tgtctatttt gagggtgatt gtgtgggttg
    12661 ttaagatata cttcaaaagg gaaggaggca cgtagcttat aattaccatt tgatagccat
    12721 tcattataca taaaatccca cgcatcacta tattcagatt ttgaaatatc aaaatgtccc
    12781 acaagatatt ttccagaagg tattatcata ttgccaagat tgtcattctc tttgacaact
    12841 atgttatctg gaatagtcat gcaaatacta gtccttaatt gatgctcctg tgtaaattgt
    12901 ggattatcat gataaatggc taatatttgt gtgttttcaa gtccaagtag attctgctca
    12961 cttgcatatg tgaataaccg ttctaaaagt tttgggaaag tactggttag attaccatat
    13021 gaaccagtat acctagtgta aatagtattc atattatcaa gttctagaat ttcaatttca
    13081 cctttaacct ctctagtatt cttacattta ggttttgata tgcttctatt gtactgagaa
    13141 attttatatg gttctttgca attcttgcta taattgtttc tatattttat agggcttatt
    13201 ttatagtaat ttttaaatgc tcttgagaaa actgctgaat cagtaaagcc aaagtagtgt
    13261 gcaatatcag taacagtcat gtcagttcta tgaataagaa aagcagttgc attttctagt
    13321 tttagcctat ttacatattg tgccaaagtt tctttttcta ttgctttgaa gattctatga
    13381 aaatgaaatt ttgaaaaacc tgctatatta gaaagttcat caagagaaag aggttcatgt
    13441 aaatgttctt ctatatagtc ttgcactttg caaatacgat ttagatattc ttttttactt
    13501 ttagcatttg tcatatatag ccctccttat tgtgtaaaat gtgtatcaat taatttatag
    13561 caatatagta ataaaatttt atgtttttgt aatttatgat aaataagata gcgtttaatt
    13621 ttttataatt gataaaagat atttataatt aataaaagat ataagatgcc ttatatttat
    13681 ctttgcatat aagtatagtc ttcgtaataa ttagtatacc atagtgattt atttttaatt
    13741 gtgtatttat ataaatacac aacttatgat atggacgaat tattatgcaa ataagctgta
    13801 attaaataaa ttgcaatata aaccagttga aacataagtt agggaaatat tatcaaagtt
    13861 attatttagc ataagagtat agatggaata aaataagatt aaa
//
LOCUS       DACXMZ010000090        13828 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.091, whole genome shotgun sequence.
ACCESSION   DACXMZ010000090 DACXMZ010000000
VERSION     DACXMZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.091"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            184..1587
                     /gene="hflB"
                     /locus_tag="JD016_10470"
     CDS             184..1587
                     /gene="hflB"
                     /locus_tag="JD016_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAU5000060.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(1975..2997)
                     /locus_tag="JD016_10475"
     CDS             complement(1975..2997)
                     /locus_tag="JD016_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAU5000061.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3032..4630)
                     /locus_tag="JD016_10480"
     CDS             complement(3032..4630)
                     /locus_tag="JD016_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087158.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HAU5000062.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4672..5937)
                     /locus_tag="JD016_10485"
     CDS             complement(4672..5937)
                     /locus_tag="JD016_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAU5000063.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6081..7508)
                     /locus_tag="JD016_10490"
     CDS             complement(6081..7508)
                     /locus_tag="JD016_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HAU5000064.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SVGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQMYMGVEE"
     gene            complement(7546..8553)
                     /locus_tag="JD016_10495"
     CDS             complement(7546..8553)
                     /locus_tag="JD016_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HAU5000065.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            complement(8750..10072)
                     /locus_tag="JD016_10500"
     CDS             complement(8750..10072)
                     /locus_tag="JD016_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000066.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(10430..12070)
                     /locus_tag="JD016_10505"
     CDS             complement(10430..12070)
                     /locus_tag="JD016_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HAU5000067.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            complement(12226..12882)
                     /locus_tag="JD016_10510"
     CDS             complement(12226..12882)
                     /locus_tag="JD016_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000068.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            complement(12908..13609)
                     /locus_tag="JD016_10515"
     CDS             complement(12908..13609)
                     /locus_tag="JD016_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HAU5000069.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     KKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLD
                     IAMLIGMFGTQIILS"
BASE COUNT         4687 a         2615 c         1656 g         4870 t
ORIGIN      
        1 atactttatt caatacataa gtagaaatct ctatatacat aaattcattt tttcatacat
       61 cttacaaatt tatttttatt tagataacat gtatattttt tccatgaaaa ataaataata
      121 aaaaatagta aatatggaaa atataaaatt gattaacaat aattaattac ctgaggtgtg
      181 aatatgaaaa tgctaaacaa cttttttaag aaaatcaaca atccagctcc tgtctttgca
      241 cagacaaaaa tggaaagaga taatcaaaca gatgattcac tttctacaaa accaaagact
      301 acatttagag atgttgctgg attagatgaa gtaaaagaag aactatttga aatagtcgat
      361 tttatgaagt caccacaaaa atatcaaaaa atgggtgcaa aaatacctaa aggagtatta
      421 ttttatggtc ctccaggaac aggtaaaacc ctacttgcat ctgctgtagc tggtgaaaca
      481 aactcatcat ttttcaatgt tactggttca gagttcgttg aaaaatatgt aggtgtggga
      541 gcaaaaagag ttagaactct atttgaaaaa gctagaaaag aagctccaag tataatattt
      601 atagacgaaa tagatgctgt aggagcaaag agacatcttg aaagtaacaa cgaaaaagac
      661 caaacactaa accaactttt agttgaaatg gatggattta ataaagattc caatgtatta
      721 ataataggtg ctaccaatag acttgactta ttagatgaag ctttacttag accaggaaga
      781 tttgacagac acattcatat aggtgcacca aactatcaca ctagatttga aattctaaag
      841 gtacatacag atgacaaacc tatagacaaa tctgtaaatc tagaactttt agctaaaaaa
      901 actcatggtt tcaatggggc tcatctttca aatatagcaa atgaagcagc tatatttgca
      961 gttagagatg acagtgagtg tattacatca gaacattttg acaaggcact tgaaagagtt
     1021 attgcagggc tagaatctaa aaactctgca ttggttgaaa aagagaaaaa aatagtagca
     1081 tatcatgaag ctggacatgc tcttgtaagt gatatagtag gaatttgtcc tatacaaaaa
     1141 atatctatag ttcctagagg acaagcatta ggttatgttc ttcaattacc tgatgaagat
     1201 agatatattt atactaagga tgagttgatt ggaaaaataa aaatattact tgctggaaag
     1261 gcttcagaag aattaatatt taatcataaa tctactggtg caaaggacga cttaaagaaa
     1321 gtcactgaaa tagctaacca aatggtttgt gaatatggta tgagtaactt aggatttatg
     1381 actattgatg gaaatgataa gactttctta tgtgataagg ttcaaaagga agcaaatcgt
     1441 atagttgaaa tttgttataa agagacttta gagatgttaa aggataatct tgaagattta
     1501 cattctgttt ctaaattctt atttgaaaaa gaaactatga ctcatgaaga gttaaaagat
     1561 ttgataggaa aagaagctgt aaactaatat agttttaaaa atttgaggct gtctcaaaat
     1621 agagataagc ctctttttat tgttaaagtt ataaaaatac atttgatttt atacaaaaaa
     1681 gtgcctcata aactaaaaat ttcattttga gacacttaca tattttaatt ttgtctattt
     1741 acagaatatt atatctacat aaaactaaac tgatgtaaac ttaactacct ccagtaatgt
     1801 ttgaagtggt ataaaagcta gtagcctagt ttctacactt ataatttata ttttacataa
     1861 ctaaatttag tagattttta tactcacaat ttagaacgat tgaaaactta aaaatttctt
     1921 atcagatatc tacaacttaa ttaggtatct accacttaat taaatatcta ccacttaaag
     1981 tgtagaacct ctttcaatca attcaggttt taacatttca acaactgact ctggattttc
     2041 ttcatttaat aaggacttaa gtatgttaat tgttttttca atcaacaaat ctagtggctg
     2101 tctaatcgtc gttaaattat aactcttcca tgaagccatt tgaatatcat catatcctat
     2161 cactgataaa tcctctggca ccctcaactt gtactcaaac cttgcagcat ccattacacc
     2221 aatagctatc aagtcacttg tgcaaaaaat tgctgttgga ggatttgata atgctaacat
     2281 tctcttacct gcttcatatc cagaatcata atcatagttt gcagattctt ctttaaggtt
     2341 atgaatccca ctttgtctta acttagaata aaatccaatt ttcttttcca tttcttcact
     2401 tgtttctctc gtaaattgaa tatatcctat attcttatga cctttcttaa gcagatattc
     2461 agcagccatt cccccacctt caattgggtc tacatagact gtacttattt ttataccagg
     2521 tataaaacga ttaaaaagaa tcacaggaat attgttttct tcacaagtct cagccatcac
     2581 cttattcatg gccgaagctg taataatcac agcttctact tgaaactgaa ttacttttga
     2641 aattatacta tctatacttt gttgtctagg aactttaaaa acgagacact gtttacctat
     2701 ctcttgaatt ttttctacaa ataaattgat aattctattg taaaatggac ctagactatc
     2761 accaactatc aatcctataa cattagtttt tccactaatc attccccgtg ctaccaaatt
     2821 aggaacatat cctatttcgt ttgctacacg aaaaattctc tctcttactt caggttttac
     2881 gccctttcca tttgcactac caaatgctct tgaaacagat gattgagata ctcctgccaa
     2941 tcttgcaaca tcttttgagg tagccgattt attataatga actttctttt gattcattta
     3001 gtatttctcc ttaaatatct agtatttctt cttaaaattg tatcaaaaat aatataatat
     3061 attggataat aaaaaaagct aatacaatta gttcttgttt acctaaagaa aatggcacta
     3121 tagatactcc atctacttta tccattcctt gagaaataag gtgatttctc ataccaaatg
     3181 cagtgtattt tttaatatca tctgcattat gatttttctt taaaatgaca ctaaaaatat
     3241 atccaaatat caatgttatt gtagcaccta ttgctggatt ccacaaccaa ctcacattat
     3301 atttattaga aataaaaaat cctaaaacta accctacaat aaaaccaata gatactcctt
     3361 tatcattagc tttatatgta aacattgcta gtacaaatgc acctgtagct ggtccagcaa
     3421 agtatgagat atagttacca acaaggtcaa gaattgattt gatgcttcct ccaaatccca
     3481 taataataaa acctgtcata acaactccta taactacagt tataatcatt gtaactttta
     3541 atgatgattc tttgttgtct tttttgaaat aaaactcata aatatccttc gtaaaaacag
     3601 ttgtcatact gtttaatata gagtcgatac tagacattgc tgctgcaaac acacctgcaa
     3661 taataattcc aatggctcct actggaagtt catttaaaat aaaagtaatc attatttcat
     3721 ttgaagtttc aaatgtcctt cccccataaa aaacaaataa aattactcct accaatagca
     3781 taaagtagta aactaaattc ataacaaatg cactaactac aaaagaattt tttgcacatt
     3841 ttaaagattt agatgttaaa actctttgta cttgagattg gtcaaaacaa aagtatctta
     3901 tccacattac agttccacca attaaagtag cccaaaaagt atttgtcact gaaatatccc
     3961 tactgaaatc aaatgtattt aattttccac tctgttgtgc aatttgtaat gtatcaaaga
     4021 aacttagatt cattcctttt aaggctacaa taattactat aaatacagca cttattacta
     4081 ctatcatttg aatactatca gtccatataa ctgccttaat cccacctaat agagtatata
     4141 taattgatac aagtacaact attgggacta aagcataaag tgaccagcca gtaatcattt
     4201 gaataataag cactggaata tataccatag aactagcttg aatcaatgaa ttaataaaaa
     4261 attgaataat agttaatcct cttgaataat ttcctaaacg aagtcccata tattcatata
     4321 tagaggttat ttttaattta tagataactg gggtggtaat gctcagtgca aaaaatattg
     4381 ctaaaggtac tgtaatatta accattactg gatacattcc atctacatat gcccatccag
     4441 gaccacctat aaagccattt gcactaatca tagtagctgc aactgataaa gcaattggaa
     4501 tccatggcat tgacctacct gccaagaaat aatcttcttg atttttatta tcttttcctg
     4561 aataataacc aataactaac attaatagca aatatcctat tataatacat acatcaattg
     4621 atgaaatcat aagccacccc tttctaggta acaaaataaa ttcctagtat tttatttttt
     4681 attatttaac gtctccggta agaataacca tacaatagca cctgctacaa agactgcgat
     4741 tgatatgtaa aagatagtca tcatacctaa agtaccagaa gctgtaccag ctatagcagg
     4801 tattgcaaac gtagatataa tccttcctcc attaaatgac ataccagatg acaacgaacg
     4861 atatttactt gggaacaact ctgtgtagta tgctccccat gctccagaat atccaacagc
     4921 aacacctagt aatactgata tcatatagaa tgttgttgta ttatctccac ctaattttgt
     4981 atatataaaa acaaatactg ctgctaatgc catacctata aaaggtattt ttcttccaaa
     5041 tttatctgca attgaacccc agaataaata tcctactaat tgaccaattc cttgtaaact
     5101 atatatagac ccagccactg ctgctgacat tcctattcct gtcactaggt atgtagttgc
     5161 attatttgaa aatgctgaat atgctgtaaa atttgcacct gataaaagaa tcgttcccaa
     5221 tccaatccat aagtattttc ccttatacat acttatatag ctaatctttt caccagtttc
     5281 ttttcccttt ttaactaatt cctttgtata ctccaaatca tctggcacaa agaaaattgc
     5341 taagatagct ccaactatag gtggtataat cgcagtatac atcaatattt gccaatttgc
     5401 atcacctaaa acgccatata ctttcccacc aaaaatcatt gctaatgaaa ataaagcagt
     5461 taatatagca gctaacttac cacgatattt acttggaaac atatctgcaa ccaaaggcat
     5521 agctgttcca aatacgcctc ccaacactac tcctgtacac aaacgtgcta ttttccataa
     5581 cataatattt gtaggagata acattggtat aaatgttgtc aatccaatca gtaaagcaac
     5641 gctaattaaa gtcctcttct tcccaatttt tgtagaaatc accccaaaaa taaatgtacc
     5701 tggaattgta ccaaaagaca taactgcaaa tatttcttct gtttgtttta atgaaaaacc
     5761 aaatccccca tctgctaaag cataaatcat tgttggttta ataaaagtac ccaccataaa
     5821 attgtagcaa taaaaaaagt atattaaagc aattgaaaaa aatgcaattc ctctttttag
     5881 gtttgaatag tcttttccta atatgatatc attttctatt tcaaccgttg ttttcatatt
     5941 tttataattc ccccttttaa atttagtcat aattttgcta aaagtttaaa catctatttt
     6001 tcatatacat tccccactta aattttataa agcaatactt ccctatatac atttcaaaac
     6061 tatttatact ctaaacagct ttattcttct actcccatat acatttgttt taatacatcc
     6121 ataccatcaa aagttatcca ctcctccttt actttacctt gtagtatatg ataatgattg
     6181 attcccatta cttctatatg ctttccagaa ggctgtccaa aaaatcctat accttcattt
     6241 attccacgta atctccatct tacagataca tcataacctt catttttttc tctttgatta
     6301 caagttactc tttccacttc ataacttcca tttggaattg atgcaagtaa acttacaatc
     6361 attccttgaa tctcatcata accatttaaa tctttatcac atataaaatg tactactgca
     6421 ttatcatgat agtatttttt aacttcattt atatatttat aattataaat acggcttgtc
     6481 atttcaagca tcatttcgcc aacagaatca tcttttgctt gatacttagt tggcataaat
     6541 tgaccatcca tagattcaca gataccataa gttgattgta aactaagcac tttagattca
     6601 cttgcctttg ccatactctt tgctacttcc tgagggttta atcctaattg agtaacaatc
     6661 cataaattat ctctaaccaa ccattcttca tggattctat tgttagttgt ggcacaatca
     6721 attacagttc taaagtttat ttttctacct gttgctggtc caaaattact atcacctaag
     6781 ttagttgctg tagacaaaac acggtgagaa gataaaaaac catcagcacc aaagttagac
     6841 catattacat tttgaccaat taatcttcta tcaggaaacg catgtaatgt ttgaagagta
     6901 ttggaaataa catctttaat accaactaaa tttgaagagc cacaatgcat tgtaacatta
     6961 ttgtgatatg tatcataaat aacacctata cctttttctt cccagatatt atgtgtaatc
     7021 ttcataatat aatctacaaa atctctatac ttagaatcaa atccaggtat ttcctgttcc
     7081 ctttttgtct gttttgaaaa atcattgtaa tccattactc caacaggcgt tacttctaat
     7141 gaatctccat agtaaatttt tgtagatgac atattatctt cccccttaaa tataactttt
     7201 ttattttttt cagtaagagt atttgtattt aatttatctg tatccactgt atctatgtta
     7261 actttattta tgtcggattt atttgtgttt gttttatcta tattcccttt atctatattt
     7321 cctttatcta tatttctttt atatgtgttc gttttattct catttgtttg attttcattt
     7381 atattactaa aattagtttg attcatttta gctttgtttt ctttaaccgt acatgattca
     7441 agcccacttg tagaaacttc tttttcttct tctttacttg ttgcattatt tttgttggta
     7501 gatgccactt tatatccccc tttattttag aatacgtatg cataactaag cattaaaaat
     7561 acttttagtt ctctcaagaa tatcaagccc ttgttgtttc aaccaataag gaatatcaat
     7621 taacacccag ttttcttgaa gtttatcgcc cttacgataa taaacatcaa caacttgcat
     7681 atctgcttta acctctccac ctggtaatcc taaaaaacct ccacatggta cattagtcag
     7741 attaggccat ccaaagaaac aagcaaaatt accttctgca aatctacata catgcccatt
     7801 aaaagtcttt ccttttaaat tatttctaaa tggtagctgg tgttgctctt gatatctagg
     7861 tatagtataa gaagcaccaa ttccacctgg accataccaa atcatatctt tagcccaaga
     7921 tttttcaagt atttctggtg ggcatcccat agcaccactt tcatttaatt cagataaatc
     7981 atctaccatt ttatttacta atgctaaagt ttttactcct tcaacttcat ctgcatcttc
     8041 aaaaagtaat ccattatggt caattggccc tggatataca aaatatgaac ctgtttgtgg
     8101 tggcaatgga ttaactcctg cttgaatcat aaacccaatt atatctacaa ataatccagt
     8161 tttagcaatt ttaccatctt gaacacagtt aaattctgca tatcttaaac taatcatttt
     8221 gcgagtatgt ttaatgccaa gccaatcctt atcaaaaagt cccataaaat gccccatact
     8281 cattacccat tcactattgt caatctcatt tgttcctgca atgaaaatat cttgtcgtct
     8341 ttgcatatta ctcaatgatt tttttaaagg cttccaaaat acatctgcta cattttcagt
     8401 gccttcttgt tcacgaaatg gatacacacc tttccagtta taatctgatt tcatatgttg
     8461 ctttaagaca ttttcaacct catccccagt agcattttct aatgcttcaa aataatttct
     8521 tacaactttt tttgaatcct gatattttcc cataattatc tcctcccaga tttatttaag
     8581 ttataaatta actttctcat tttatgcata cgatttcctt tttaatgatt ttttagctgt
     8641 tttaagttta ttttatgaat acatatgcat ttttctaaat attaacatat atctaaaaaa
     8701 ttgtaaagaa aatgttatca taaaaattta atttttgact tatatctatt taaaacaaca
     8761 gaaaaggttt aaaacgtaat attctactgg gtctaccata cttgctcata agctgttcac
     8821 tcactacctc acaataacca gaatcacaca atctaagtat aatcctgtcc atagttctaa
     8881 tacttacacc acatatagta gctaattcct ttgatgtaaa ttcagttttt ccactattta
     8941 ttattttatt gaatacttta tatacagtat tagcacttag ccctgtctct gtagcaattt
     9001 gataaaaacg ttcatcaaaa ctatttttct ctttgacagt aggatttggc tttagtggtc
     9061 ccataatttc tccattttca aaaactacat atgctgcatc atttttttga ctttgagcca
     9121 tcttaatact ctcataagcg ttaaatttag cttcatttgc tgtttttcca taaccaattc
     9181 ctatataagt gtttataact gaaacttcac gaatcatatc caacaaataa atatctctgt
     9241 atttctttgt ttctaactca agtatttttt tagttgtaaa aatcataaat tcacttctac
     9301 tctgctctac caaaacacca tctatacggc tattgaaatg gtatagttta tcaagaatct
     9361 taatcctttg tgaaagataa gcgtactcat cttccttcat tagtgaatat tctgaaggca
     9421 tgttcagttt aatagcaaga acaacaatct gattctcatt gtttttcttt gcaatataac
     9481 gcatctgaat gttttttata ctttgcacca ttacgtcctc agaaggcaag gctaaaacta
     9541 ctggaatatt atcttttttc attatatgat aggcctcttc aagacctgta atacaacaac
     9601 atacatcatt attttgataa ttatatttat gaaattcaag cacaaacttt atatagtcct
     9661 cttctaaata cttttgctct gcaaaaaaca tatgttcatc cttaaattca actccaattt
     9721 ctttatacaa tctcttcatc atagccttat cataagtatc tatactaagt ctactaatat
     9781 cgaatttaag caaatatgta atttcaagca acactctgta caatgttgtt tcatgtctag
     9841 gaatataatc agacaataca aaatcttttt gtcttttttc aaaaaattta aatggcgact
     9901 tacctgtaaa aagaactgca tccactctac ctctatattt ttccaataaa tcaggtactt
     9961 caatatatct atcatatcta agaaaatctg cttcaatatt aggaaaattc tttttactta
    10021 ctgctagtat tttatcgatt accatacctg gtcctacata aagtattttc atttcgtctc
    10081 tcctttccta atgatactat tatctcttaa aacattttct aaacctacca tgactaaagc
    10141 cacaggactc ctgattcata gaattttgaa taatcaatta tacctaatat aaaaagttac
    10201 tttaagtttt ttaattacga ctatctgttg aaacattttt gtatttaaat ttttgtattt
    10261 aaatcttatt cattaatatt aattacattt tacaattaat atattgtttt ttctacttaa
    10321 agttaaattc tttagaaaac acaagtagag taatctttat taatttaaca aaaatagcct
    10381 tccttaatat tcaaagaaag actattttta taaaagttct taatgtattc tattttgcta
    10441 tgctaagagg atttttaatt acacgtccag cacatacatc agtttgcttt cctcctgcca
    10501 atgcaatttt accattcacc aacacatatt ccactcccac tgtatactgt ttaggattac
    10561 tataagtcgc tttatcttct attgtatctg ggtcaaatac cacaatgtca gcaaaataat
    10621 tttctttaag tagacccctt tctttataaa tgcctaagcg ttttgctgaa agataagtta
    10681 tctttttaac tgcttcctca aaagtcaaca tacctttttc tctaacatag taagagaaaa
    10741 ttcgtccaaa agtaccaaat gcacgaggat gaggctttcc aagagaaagt aaaccttctg
    10801 tagaaaggtc catactatca gaacctatac atgactttgg atggcaaaca actttatctg
    10861 tatcctcttg accttgtgta tgaacaacta cttgaatttt acttttttct tcaatcaaaa
    10921 gcctaaagaa cgtatcaaat ggattttcac cattgatttt ggctacctcc attaaagttt
    10981 taccctctac atatttattt gcttcagtca ttacagaggc tagtacaaca ccgttccacc
    11041 cacctgctaa ttggtagaaa cattgccatc caggaatccc ttcctccaac tcttttataa
    11101 ttctgtctct attttcttgt acttctaatc gttttaccat attttctaca ccgccttcaa
    11161 aagcccaagg aggtaaaagt gcacccaaag atgtcattcc agctgtatat gggtaacaat
    11221 caaaagttac ttccaaacca tctgcctcag cttcatctag ctttttaaat acctcatcaa
    11281 tagttccaaa attcttatga cccataactt ttaaatgaga aatatgtaga ggaactcctg
    11341 cagtcttagc tatataaatt gcttcatcaa gagcttccat ttcttcatca ccttcatctc
    11401 taatatgaga tatatagtat ccattgtatg gtatcaattc cttacataat tcagccattt
    11461 cttctttctt tgtgtatgct cctggtaaat aaactaatcc tgatgaaaga gctacacatc
    11521 cagcttccat agcttttcta acttccaact tcatattttt aagttgctct aaagtagctg
    11581 gttcctttga aaatcccatt tcattgatac gtacaggtcc ttgtgctaca taagctacaa
    11641 cattgactgc tggagaattt tcagttaagt aatcaaagta ttcttgcatt gtattccaat
    11701 gaagctctaa ttttgttgga attgtaccaa ttatcctagt tccaagataa tcaattaact
    11761 cttgcttctt ctcttctctt atcggagcaa ctcctatacc acagtttgca ataacatctg
    11821 ttgttacacc tgtatggata cggcttcttg tgtatttatc atacacaaga gataaatcag
    11881 aatgagtatg gaaatctata aaaccaggtg atacaaattt tccagttgcg tctatagtct
    11941 ctttagcctc tttttgactt aaatctccaa tagccatgat tttgtctcct acaatcccta
    12001 catcactttt atatcttgaa tttcctgtac catcaacgat tattccattg acaattttaa
    12061 catcaaacat gctttttctc ctttcaagac ttatttatat tcatatttct taataaacaa
    12121 tcctattctt ttttctcaac aaataattct agtctttctc cttaataaac aatcctattc
    12181 tttttctcaa caaataattc tagtctttct ccttaataag caatcctatt cttttgtttt
    12241 attgttatca attacaaaga agacccttgt gataagtatc ccacctaaca caataaacaa
    12301 tgttaataat ccacctggta taccaatttt ttcaccaaag tacataattg ctaacgctac
    12361 agcaatcgta catccaccag ctcgaatatc cttacgtgca atatcaacat aaattgctcc
    12421 aaataatgct ggaagtatat aattaaatga attagtaaca aattcaggta acaaaggaat
    12481 tactttatag cctacaatgg taaaaatggt aatcatagaa gctgatacaa agattgaaca
    12541 acatgtacca attgttccaa caacttcacc ctcatgagtt cctgcttcta cacctgcaat
    12601 tttctgtgcc attgatgctg caggcatacg tatatctcca acagaacctg caagccaacc
    12661 tatataagaa cctggagctc ctagtgtagg ataataagct ataggttgaa caatccaact
    12721 aaccccataa gtcgcagcga ctaaccccca aatttgaaat ataacagaaa gcattggcat
    12781 ctgaccaaag cgaataccta catagatagc gggaataaag tttgcaataa ttgcaagacc
    12841 tatagaaatt cttcctattt ttatcatctt tttatcatac ataattatca cctcttatcc
    12901 gttttaatca cgataatata atctgtgtac caaacatacc aattaacatg gcaatatcta
    12961 aggataattc ttgtaatcgt tgattctttt taaataaagt tgtcaatact agcataacta
    13021 tagctgaaat aattgctggt gtaagttttg gtatttcaaa tgtttttatt tgatttgaaa
    13081 gaaggtaagc aaataatcca actgtagctc ctgcaagtag ttttttaacc caagtaggat
    13141 tatacttcat gttcatcttt tcaacaattc cacgcattct atgacccaaa ataaatacaa
    13201 caaatagcca acctaagtta tttaaagcca tcccccatag tgcatacgaa aaagcttctg
    13261 ctccaaatgc agcactacct acttctaccc cagataaatt ggatgtaaga gatattactc
    13321 caagctctgt tctagctgct ccgacatcac agagcctcat ccaagtcgta ggagcgccaa
    13381 tcacagctac cagtgataat aatgtaataa caggagatag agaaggtcca atggctgtaa
    13441 cactagctga gcgaattgct gctttaattc ctttcttttc aataccaaga tttgaagcct
    13501 cttttagtgc ctctttcata aatacgattg cttgaacaac tgaaataatt actaaaaaac
    13561 tagaaacaat ccaaagacct gggctattaa tcatttcttg aaacttcata ctttattcct
    13621 ccttaaaata aatttttcag cattttattg tgagctttct ttctgtaatc ttgttatgtt
    13681 tttatattgt tttaatttat tttatctaag atatagtatc tttaatagga gattatctgt
    13741 cttaaagtct cactaaaaac attaatactt aaatatttta ttcataatta gtaaatatca
    13801 gaaaattcac atattatttt tttaacct
//
LOCUS       DACXMZ010000091        13609 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.092, whole genome shotgun sequence.
ACCESSION   DACXMZ010000091 DACXMZ010000000
VERSION     DACXMZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.092"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(482..1225)
                     /locus_tag="JD016_10520"
     CDS             complement(482..1225)
                     /locus_tag="JD016_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAU5000070.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DKLIMNYPKKLINHFHQKDKLLDVDYTNI"
     gene            1391..3958
                     /locus_tag="JD016_10525"
     CDS             1391..3958
                     /locus_tag="JD016_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU5000071.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIVKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            4188..4592
                     /locus_tag="JD016_10530"
     CDS             4188..4592
                     /locus_tag="JD016_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAU5000072.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NRDGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4616..5113
                     /locus_tag="JD016_10535"
     CDS             4616..5113
                     /locus_tag="JD016_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU5000073.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            5116..5517
                     /locus_tag="JD016_10540"
     CDS             5116..5517
                     /locus_tag="JD016_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HAU5000074.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5535..6023
                     /locus_tag="JD016_10545"
     CDS             5535..6023
                     /locus_tag="JD016_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU5000075.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            6050..6805
                     /locus_tag="JD016_10550"
     CDS             6050..6805
                     /locus_tag="JD016_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAU5000076.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6805..7668
                     /locus_tag="JD016_10555"
     CDS             6805..7668
                     /locus_tag="JD016_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAU5000077.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7715..8194
                     /locus_tag="JD016_10560"
     CDS             7715..8194
                     /locus_tag="JD016_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000078.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            8259..9566
                     /locus_tag="JD016_10565"
     CDS             8259..9566
                     /locus_tag="JD016_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAU5000079.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYILVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9876..10016
                     /locus_tag="JD016_10570"
     CDS             9876..10016
                     /locus_tag="JD016_10570"
                     /inference="COORDINATES: protein motif:HMM:PF09951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HAU5000080.1"
                     /translation="MNGEGCILSNKITKECYKIGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            10528..11622
                     /locus_tag="JD016_10575"
     CDS             10528..11622
                     /locus_tag="JD016_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5000081.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            11627..11827
                     /locus_tag="JD016_10580"
     CDS             11627..11827
                     /locus_tag="JD016_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HAU5000082.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            11827..12744
                     /locus_tag="JD016_10585"
     CDS             11827..12744
                     /locus_tag="JD016_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000083.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGRTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12741..13499
                     /locus_tag="JD016_10590"
     CDS             12741..13499
                     /locus_tag="JD016_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000084.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
BASE COUNT         5219 a         1424 c         2489 g         4477 t
ORIGIN      
        1 ggggctagag tctggaaatt tttatttaaa gaatacacat aaaaatttct agactctcat
       61 catttaaaag aagtattcta aatttattat ttataattaa atattatctg aaaaaatggt
      121 atttttatac ctttgtttgt tatgcataat tttacttaga acataagctt tatataaaat
      181 aattaaaaat atacctaatt aaaataagta aagaaagagc tatcatagaa caaatttaat
      241 ttgctcatgc gacagctcct ttttagatga tgctttttta gatttatact ataaggtata
      301 taaaactaag cttattaaaa atgttataat ttctattgct atcatagacc atatcaatcc
      361 atcaagttca taaaagtaat taagctttat taaatagctt ctttattata atttctttat
      421 ttcatataat ataaatagta ttaaaatttt tatgggagtt aaagtaagca tgtattatta
      481 cttagatatt tgtataatca acatctagta atttatcttt ttggtgaaaa tgattaataa
      541 gttttttagg ataattcatt attaatttat ctggaaaatt aatttccttt aagatatttt
      601 cagaatactc atagttacct atgtcaaaac aaatgtgagc atctgaagtt agtatgattt
      661 tagcaccata ttttttgcaa agtgtagcaa tatacttaca gttatccgag ctaccatttc
      721 ttgacacgcc tttaatagaa caattattta tttctattaa gatattcttt tctatagcta
      781 atttaatgat taattcatag tcgattgggt agtttgggtt tccaaggtgt cctaatattt
      841 caatattatc atgcttattt attgcattta ttaaagcttt agtattattc tctaatgtat
      901 ttggagaaaa aacagcttcg tgaaatgaca agataagata atctaaccga ggaattacaa
      961 aaggctctag gtctatgttt cctttaatat ctagtatatt tgcttcacag cctcttaaaa
     1021 ttataatgtt atttatgatt cttgggatat tttgaatgtt gtgaaagtac catctgtggg
     1081 gagaaccagg catcttagga ccatgttcag atgtacctag tatcttaatt ccattttctt
     1141 tacaataatt aatattttct agtaaagtgc tatagccatg accactgact atagagtggg
     1201 tgtgtaaatc tgataaaaaa ttcatataaa ttcctcctct ataaaagatg agggtaacgt
     1261 aatttatatt ataaaacgaa taggaaagaa aatcaatagt tacataatta gacaaaaata
     1321 ttaaaatatt gtagaattaa gcttaataat aaatgtttaa cagtattact gcttataggg
     1381 gaggttaact atgacacaaa agaagctatc aaggaaagaa aaagttttaa tttgcttgaa
     1441 aaggttaaca gaagaacact tgaactttaa taatggcatt agatttggat ttgatgcaga
     1501 atatatagct actgaagtag gtataaatag aagtaatgct agtaaggaat taaatcaatt
     1561 aataaaagaa agtattatta taaagataaa gggtaagcct gttcagtact tatataaaga
     1621 aagtattgag agtattttag gaaagaaact aaataaaggg atatttgata atattaactc
     1681 tataaacgaa tatagtgaaa atgattctat caaagatgag tatattttag agagtataaa
     1741 aaaagagaac aatattttta atttagtagg atgtgatgga agtttaaggt cccaaataga
     1801 acaggctaaa gcagcagtaa tatatccacc taaggggtta agtacattaa taataggacc
     1861 tacaggagtt ggtaaatcta tatttgcaga atatatgtat aagtatagtt taactataaa
     1921 agaagatagt gataatgcac catttatcat atttaactgt gctgattatt ctgataatca
     1981 gcaattatta ttagcgcagc tttttggata tgtaaaagga gcatttactg gagctgataa
     2041 agataagtat ggtattgtac atgaagctaa taatggagta ttgtttttag atgaagttca
     2101 tagattatct gcagagggac aagaaatgct atttctgctt atggataaag ggatatatag
     2161 aaggttagga gaggctaata aggtacacga gtgtagagtt atgataattg cagccactac
     2221 agaagaccca gaaacagcaa tgttggaaac gtttttaaga agaatacctg taactataaa
     2281 gattccgtct ttagaggaaa gaggctttca agagagaatg aagcttatat gttatttttt
     2341 taaagaagag agtgttagga ttggtgttaa gttaaaggtt tcaaaagaag ttattaaggc
     2401 atttatttta tataagtgta agggaaatat aggccaatta aaaagcgata tacaattaat
     2461 ttgtgcaaga gcttttttag attatatgac ctataaaagg gaatgtgtat atgtaaggct
     2521 ctctcttcta ccaaataata ttagagaatc attatatgga aataatagaa aagaggagtt
     2581 tgtacaaaat ttcagtttta ttggaaaaga tgatattatt tttttaccag aagaaaagga
     2641 ttttgaaagt gaaaatttat taatagaaaa taaaaaatat gatttagatt tttatggaat
     2701 gataaaagaa acttggaata aacttcaaca agaaggtatt aaagagtcac aaattagaag
     2761 tgttatagat ggagacatac agaagtattc ctataattta atgaatactt ttatatataa
     2821 tagtagtaat caaacagctt ataacaacat tgttaatgat tcaattgcaa ctactgtaaa
     2881 atatatttta caaaagcatg ataaatggag caaaagagaa ggattagata aattaataaa
     2941 agctatagct ttacatattc agaatttaat tgaaagaata aagattggaa atatagtaaa
     3001 gcatcctaat aaagatgaaa ttatgaagga aagagcatat gaatataaca tagcaaaaga
     3061 aatattatct aacatgtcat taatgtatgg agtagagttt ccagaagatg aagcaatttt
     3121 tttagcaaca tttctttatt tatcgtatgt gggattaaat gaggagagta tagcaatatt
     3181 agtcataatg catggtgaat ctacagcatc tagcatggtg aatgtagcca atactttact
     3241 ggattgtaac catgcagtgg caattaatat gggattagaa gatagagttc aggatatttt
     3301 agtacaagct gtagaaatag ccaataaaat tgataaagga aagggaattt taattttaac
     3361 tgatatgggg tctattttaa catttgctaa agttattcgt gatgctacag gaaaagaagt
     3421 aaaagcaata gatatggtaa gtacacctat tgtaatagaa gcaactagaa aggctctaac
     3481 tccagaaatg acattagaga aattatattt taatattata gaatctataa agaaacatta
     3541 tggagatatg gaagttacat atgctgaaaa agataatggt ggtagatatt ttgatagatt
     3601 actaattgat aatattagta aaactttgac ttttttaaat ggggaaaagg cttattttat
     3661 attaaaagaa gtaattaata gaattagtga acactattct ttagtaattc aagatgaact
     3721 tttagtaaag tttatatttc attgttcatg tatgattgaa agagtaatag taaaagagtc
     3781 tttagtttat aaatcatatg aagaaagaat tagtagaaat aaagaattat atttagttat
     3841 aaaagaaagt tttaagctag tagaagagac attcgatata attatttcag atatggaata
     3901 tgcaaatata ttagatatat ttgaatcaca atatgataca gataaattca aaagttgata
     3961 caaaatttaa aaatagccaa aaggctatct tttttttact ttttttgata cagatttgat
     4021 acaggttgaa aaaatctttt gatacatatc gaaataataa gtttttaaaa aaaattgagt
     4081 aaaaagtttt gattaaatcc tgtaaaatag ccatttgtta ttgatacaca taagttggca
     4141 cggcaattgc ttatataaaa agtgacaaga aaagtgaggt gaaattggtg ataggaataa
     4201 tagttatgac tcatgggagt tttagtgaag aaattataaa aagctgtgaa cttattgcag
     4261 gaccagcaga aagaacagca gcaattaaac taaataggaa tgataatatt gaagacttaa
     4321 ataaaaattt tgtagaaaag ttaaatgaat tagatgaagg tgacggagtt ttagtattag
     4381 ctgacctatt aggaggttcg ccaagtaatg ttgcaagttt aaaccttaaa aaaggtggca
     4441 agtttcatgc acttactgga gtaaatcttc caatgctact tgaagcttta attaatagag
     4501 atggaaaaag tttagaagag ttagcagaag catgtattga agcaggacaa actggtataa
     4561 acaatattaa taaagttctt agctcaatgt aatagaaaaa ggagtttgat taaaaatgtc
     4621 tttagtaaaa tattgcagat gtgatgatag attaatacat ggtcaagtta tatataaatg
     4681 ggttaagcat ctaggtgtaa aaaagatagt agtagtagat gatgaaacaa ccaatgatgt
     4741 tattgctaaa ggtttgataa aaatggcagc accaaagaat attgatttat cgatattaac
     4801 tgtaagtgaa tcaaggagat atttttataa taatcaagct gatgataacg tttttgtatt
     4861 aattaaaaat ttagatactg caaatagaat gatagaagaa gggattaata tcaaaaaact
     4921 tattgttgga agaataccta caggaatagg aaaaaagaaa atatctcaaa atgtatacat
     4981 taataaaaaa gagtttcttt taatagatga atttattaaa aagaatatta atgtatcaat
     5041 tcaaatggta cctgatgaag aagaagtaga attaaaccaa aatattaatc gctataaagg
     5101 agagtttatt tagcaatgaa taaatataag gtaatcatac tgtgccatgg aacttgggga
     5161 aaagtcttag tagaagaaac aaataaaaac tttggattgt caaatcaata tgaagtttta
     5221 tcactaagtt ctgaaaaaga tgtttctgta tttatgaaag aggttgaaga agtagtagat
     5281 aaagaagatg tgaaaattat aatatcagat ttatatggag gatctacatc aagtgttgca
     5341 atagctgttg ctataagaag aggaatagat gcaataaatg gacttagctt acagacaatt
     5401 ttaatcgtag atgaagaatt aagcaaagaa gataaattac atacattacc agaaagaatc
     5461 gtcaaaaaaa ataataacct ttgtgtggat ttaattaaag aatttaaaaa aatataaatt
     5521 atgaggtgaa tataatggca aaaataacat tagtaagagt agaccacaga ttaattcatg
     5581 ggcaagttat aacaaaatgg gtaaaaatcg ctcaagctca aaaaataata atagttgatg
     5641 acttcttagg tcaagatgaa tttatggctg acatatataa aatggcagca ccatcaggag
     5701 tagaagtagt tattttaaca gcagaagatg cgggacaagc attccaaaac aacactttag
     5761 gagataaaaa tatatttatt ctatttaaaa atgttgatat ggcaaataag gcttataaag
     5821 ctggacttaa atatgaaaag attcaattag gaggtattcc aaatgaagca ggaaagaaaa
     5881 tggtgtttac agctgtttca ttaggaaatg aggatgtaga acaacttaat gaacttaatg
     5941 aaaatggggt tgaaatagtc ctacaagtta taccagagga atcatctatg acatatgaaa
     6001 atgcgttaaa gaaatttaaa taaaaaatat aaattaggag gaattataaa tggaatctgt
     6061 tttgatgttg gcaattgtaa caggattatg gtattggttt gcagcaggtc ttgcagggta
     6121 tacccttttc tcaacactaa agtctccttt atttataggt tttagccttg gattactttg
     6181 gggtgatgta acaactggta tgattgttgg tgcaagtata gaaatggttt atctaggaat
     6241 ggtcgcagca gggggaaata ttccatcaga taaatgtcta gctgctctta ttgcaatacc
     6301 agtagcactt caaactggtg ttaatgcaga agttgcagta tctattgcgg tacctttagg
     6361 tgttatagga gtattagtta ataacttaag aagaactggt aatgctgtat tggttcataa
     6421 ggcagataag tatgctgaag aaggtaatac aaaaggcatc tggagatgtg caacacttta
     6481 ttctttaata tttgggtttg ttttaaggtt tccaatagtt tttgtatgta acttttttgg
     6541 agcagattta gttcaatcat tattagatgt tataccacaa tggttaatga atggattaac
     6601 agtaatgggc ggtatattac ctgcattagg ttttgcaaca acaatattta ctataggtaa
     6661 aaataaattc ttaccaatgt ttattatagg attttttatg gttcaatatt ttgaaatatc
     6721 aattactgca gctgcaatat ttggtgtttg tatagctctt ttaattacct ttatgaaaga
     6781 agacaaaagg gtaggtgaag tataatgcaa gaaataaaaa ataaagaagt taaaaatgga
     6841 caaggtccaa ctgaagttaa taagacaaga gtacttacaa agaaagatgt aacaaaaaca
     6901 tatctaagat ggtggtggac agcagaatta tccaactctt ttgaaagaat gcaagcatta
     6961 gcagtttgtg caagtttcac tccggcttta gaaaagttat ataagagaaa agaagattta
     7021 gtagatgcgt taaagagaca cttacaattt tttaatactc aagctatatg gggaggatta
     7081 attcacggta cagttttagc tatggaagaa gagaaagcta ctgaaggaaa aatcccagga
     7141 gaagttattt caggtgttaa gaatggtctt atgggacctt tggctggaat tggagatact
     7201 ttagattttg gtacatttca aacaatattc ttagctttag gtgcttcatt tggagcagaa
     7261 ggaagtgtta taggtgcatt tttcccaata atgtttagta tattactatt ttgcgaaggt
     7321 tattacttat ttcatttagg atattcttta ggtagagatt caattaagaa aattctatca
     7381 ggtggtattg tcaataagat aattgatggt gcttcaattc taggaatgtt tatgatggga
     7441 gcattatctg caactacagt taagctttca actccgctat catttgatat tggtggtaaa
     7501 gcaattgttg ttcaagatac attaaatatg attgctccag gtttattacc acttggtgtt
     7561 gtattcttcg tatattgggg aatgaaatac aagaagtgga caataactaa gttattagta
     7621 attttagttg tattagcatt ggtagggtca ttcattggaa tattctaatt cacagtgatt
     7681 aaaatataga atttaatttt aggaggagaa agaaatgtca gaaaaaatgt atacagaaaa
     7741 ggaatattta gaaggaatta gaggagcaac aggagaaaga gcagtatggt tctatcttct
     7801 tatgaaagaa gctgaaaaat taggagctaa tccagatgat atatgtaaag aagcaatata
     7861 tggatttggt aaaatgagag gtcaaaaata taacgtagca gatactccag gaaaaatggc
     7921 agaaatgtta tataacagta aaggacaaaa agtgtttgaa atggaattag tagaaaatac
     7981 agatgagaat ggcgttttaa aattccatca ctgtccatta gatgctgctt ggaaagaata
     8041 tggattaact aaagaagaaa gaaaagaaat ttgtagatta gcttgttatg gggattatgg
     8101 aagagttgac tgtgcacaag gtgttaaatt agattttgct caaaaatgtg ctcatgatga
     8161 tgaagtttgt gaattagtat ttacaaaaaa ataattttta atgctaaaag cctgttcttc
     8221 aaggacaggc tttaaaaaga tagaaagtag gttgatttat ggatataagc agaagtgtag
     8281 ataatagttt aaatagaacc atagaatttc taaaagaatt gataaaaatt gattcacaac
     8341 aaggtgaacc tatttcacaa tgtccttttg gtataggacc taaaaaatct ttagataaaa
     8401 ctttagatta ctgtgctagt ttaggtttta gtgttaaaaa tattgataat tatattggat
     8461 atgcagaaat tggagaggga gaggaattaa tagggattcc aatgcattta gatatagttc
     8521 caccaggaga ggggtggagt gtagacccat tttcgggagc agtaattgac aatattattt
     8581 atggaagagg tgtcatagat aataagggtg ctgtatctat gcttatacat gtattgaaaa
     8641 acatagagga tatgtatcct acaattaata aaagaataag attaattttt ggtacaaatg
     8701 aggagacagg tatgaaatgt atcaaatatt acttagataa aggtgaagaa attccaagta
     8761 tgggatttac tccagatgct atgtatccag tggttaatgg agaaaaagga agggttcata
     8821 taaggataga aaaagaaatt aaaattgaca aaagtaaacc ttacatatta gtaaggggag
     8881 gaactaaaga aaatgttgtt ccatctcatt gtactgcaaa aattattaat ggaattattt
     8941 cagagttaac tactaaagga gtagctgtac atgcaagtaa tcctgaaaag ggagaaaatg
     9001 ctatatctaa aatggtaata aaaatagttg aagataatat ggattttcag catagagaag
     9061 atatagaatt agtttcaaaa tacctttgta gtgattatta tggagatgca ttggggatta
     9121 atcaatatga tgaggtattc aaaaacacta ccttaaattt aggaatatta aaagtaaatg
     9181 aggaaaaaat cgtatgtgag ttagatataa ggtatggtaa aaatatagtt ttaaataaca
     9241 taatagatag attcaaaaag gttttttgta atggttggaa aatagaggtt attgctcata
     9301 aagacttaca ttatgtggat gagagtaatt tagtacttaa aaaattatta gaagcctatg
     9361 aagaagtaac agatgaaaat ggatatacaa ttgctatggg tggtggcaca tatgcaagtt
     9421 ggtttaaaga tatggtggcg tttggaccta agttcttagc gtataaaact ggtggtcatg
     9481 gagtagatga aagagtacca ataaatcata tacgaaagaa tatggagata tatactttgg
     9541 ctttaataaa actattagaa ttgtaaaaga agaagagact ttatgtgcaa atttacagat
     9601 atcttaaaaa gagagataaa acgagttcaa ccttatttaa atgttcaaca ccatcaaatg
     9661 gggctgtctc aaaatagaga taagcctctt acttattgtt gaaatgatga aaatatattt
     9721 tacttcatac aaaaaagagt gccttatgaa gtaaaaagtt aattttgaga caccctcatt
     9781 tgatgatgtt gaagagaaaa taagtatgaa attattaaaa aagatgattt tataaaagtt
     9841 aaggttgaaa agaaaagtta attaaatgga attagataaa tggagaagga tgtatactat
     9901 ctaataaaat aacaaaagag tgttataaaa ttggttatat gattagagaa aagtctattg
     9961 atacttatcc agatagtgga tggggattta tgacttggga tgaaaatgga gaataataaa
    10021 aattatagtt tatacagttt aataagatat atgtacaagc agttaatcta aatgattgac
    10081 tgcttttctt ttatagattt ttatataata aacaaatacc aatacatgat agttgcctgt
    10141 tataattaga ggaataaata cgaataataa ctttataaaa gaattaaatg gaaaatcgaa
    10201 gagttatatt cagtatttta aaaagaatat gaaatgagaa atgaaaataa ggtgtttata
    10261 ttaaaagagg ttttatacaa aatatttaaa aacgtgataa ataaaaactg tatttagata
    10321 gattaactat tagttttata tataatatgg attttatttg caaagtttac atattttaca
    10381 aaagacatta aaaataaatt taaatatata ttttatatac ttgtttattt gtttctctgt
    10441 ggttgatttt ttcgtcaaaa gtcaacttca aatcgcttgt tagctttaca tgtttaagga
    10501 tataattata cataaggagg gattgacatg aatgaattaa acatcgcaaa aacgctgatt
    10561 ttaaagcgta aagaaaaagg aattacacaa gatgaacttg caaattatat aggcgtttct
    10621 aaagcttctg tatctaaatg ggaaactgga caaagctatc ctgacataac ctttttaccg
    10681 caactcgcga catatttcaa cattaccgtt gatgagttaa tatgttatga acctcaaatg
    10741 atgaaggaag atattaataa actgtataat aaactgtgca aagactttac ggttaaacca
    10801 tttgatgaag tcatggatga aataagagaa atcataaaaa aatattattc atgttttcct
    10861 ttgatttttc ggatgggtct tttaatagtt aatcactatg atattgtaga tgaaaaaaag
    10921 cgtgaactat taattagtga agcattagaa atatttattc gtatacagga aacttgtaat
    10981 gacatagata tttgccgtca agcaaaaagt atggaggcaa cttgttatat attgttaaat
    11041 caacccatac aagtgattga tttattgcaa aacagcaatt ttcccatgat aaatgaatct
    11101 atacttcttg cacaggggca aatgatgaat ggtcaaatgg atgaagcgag agaaacattt
    11161 caacttggtg catatcagaa ccttatatca ttagtccaaa atctagtggg tattttgcaa
    11221 aatgcagata aattacaaat gaaggaaata gaaagacgta ttctagcaat ttcagatata
    11281 tttgagcttg atacattatc tcctgctatt atgctttctt catatttaac acaggcacaa
    11341 attaatttaa tacatggtga taatgaagga gctattaaat cattacgaaa atatgtagat
    11401 ttagcaacac gtgatatata cccaattata atacatgggg atgacttttt taataaactt
    11461 gacagatgga tttctgaaat tggaacaggt attacacgtg atgatacaat cgtaaaagca
    11521 ggtattgttg ctgctattaa aaataatcca atgttttctg tattaagtga aaacaaagaa
    11581 tacaaatttc ttatagaaaa attatcttta ttggaggaat aagattatgg aaaatttgat
    11641 ggaatactta ccacttttgg ttcctgttat tattttagat ttaatactta taattactgc
    11701 acttgttcat gtgttgagac atccaaatta taaaattgga aataaggcaa tatggattat
    11761 tgtagtactg tttattagtc taataggacc aattttgtat tttacaatag gaagaggtga
    11821 agaatgatga atattgtctc aataaatgga ttatcaaaag gttttggtaa tcgaaagata
    11881 atagataatt taaattttac tgtaccagaa gggtcagtct ttgggtttgt aggtaagaat
    11941 ggtgcaggta agactacaac aatgaaaatg gtacttgggt tacttgaacc agatagtgga
    12001 actatagatg tttgtggtga gaaagtgacc tatggaagaa caagttctaa tcgtcatgta
    12061 ggttatctgc ctgatgttcc agagttttac aactatatga gaccacttga atatctttct
    12121 ctttgtggtg aaattacggg actttcaaaa aaagaaatac agataaggag tgaagagttg
    12181 ttatcacttg tgggtcttgg aaatgaaaag agacgaatag gtgggttttc tcgtggaatg
    12241 aagcaacgac ttggtatagc ccaagctttg ctttcacgcc ccaaattact tatttgtgat
    12301 gaaccaacaa gtgcacttga ccctgttggt cgaaaagaaa tattagatat tatgcttaaa
    12361 atcaaagact caacaactgt aatattttca acacatatac tatcagatgt tgaaagaatc
    12421 tgtgaccatg ttgctatatt aaacaaagga agcattgccc ttagtggtac actttcagaa
    12481 ataaaaagta tgcacggaaa ggatagactt ttactggagt ttgcaagtaa tgatgaaatt
    12541 caaaagttta agtctagtga tggtattaaa tccttactaa aagattcaga agaaactaat
    12601 atggaaattg ttttgcatgg taaagatata aaagcaatac aaaaaacagt tatatctaca
    12661 ctcgcagaaa tgaatctttg tccagtaaag atggaactaa tagaattatc acttgaaaat
    12721 ttatttttgg aggtagtaaa gtgagagaat acatagcatt tacaaaaaaa gaatttaaag
    12781 agaatttaag aaattataaa ttatttagtt taattatttt atttctaata tttggtataa
    12841 gtagtcctat ttctgcaaaa tttatgccag acttgattgc tcattttgca ccgactttaa
    12901 aagttactgc tgcaccaact gctttagatt cttggacaca attttttagc aatatctctg
    12961 gtcttggtat gagtttaact ctaattattt tttgtaatat tttatcaaat gaatattcta
    13021 aagggacact tgttattatg ctgactaaag gtttatcacg ttcatctata gtattgtcta
    13081 aattctctgt tacggtgatt attatgacaa ttggtttttg gttgagtttt ttatttgctt
    13141 atggctatac aatgtatttt tggccaactg caaatcttaa tcatattata ttttcagcat
    13201 ttaatttatg gcttatagga attatgtata ttagtgtatt aattcttggg tgtgtactat
    13261 ttagaccagc atttgccagt gttttacttg tattggtggc aactctttta ttgagtttaa
    13321 tttctattcc taaacagatt gcaccttata ctcctaattt cttgatgtca aaaaatatag
    13381 acttgatttc agggaaagtg gcaatgccag aatttattat tccaattatt ataacagttc
    13441 ttatttctat agtatgttta ctagcagctg ttattttatt taataaaaaa ccagtttaat
    13501 aataccttta tcacaaggta aagtaatctt atgttgttaa aaactacacc taaaaatcaa
    13561 aaagacttgg acttaagttt caagtctttt tgatttttag gtgtagttt
//
LOCUS       DACXMZ010000092        13584 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.093, whole genome shotgun sequence.
ACCESSION   DACXMZ010000092 DACXMZ010000000
VERSION     DACXMZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.093"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            43..489
                     /locus_tag="JD016_10595"
     CDS             43..489
                     /locus_tag="JD016_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAU5000085.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            491..1585
                     /locus_tag="JD016_10600"
     CDS             491..1585
                     /locus_tag="JD016_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HAU5000086.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1673..2203
                     /locus_tag="JD016_10605"
     CDS             1673..2203
                     /locus_tag="JD016_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAU5000087.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASEKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            2530..2889
                     /locus_tag="JD016_10610"
                     /pseudo
     CDS             2530..2889
                     /locus_tag="JD016_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(3139..4515)
                     /locus_tag="JD016_10615"
     CDS             complement(3139..4515)
                     /locus_tag="JD016_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000088.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYNCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            4783..5955
                     /locus_tag="JD016_10620"
     CDS             4783..5955
                     /locus_tag="JD016_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAU5000089.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNINKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            5969..6616
                     /locus_tag="JD016_10625"
     CDS             5969..6616
                     /locus_tag="JD016_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000090.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            6618..7127
                     /locus_tag="JD016_10630"
     CDS             6618..7127
                     /locus_tag="JD016_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HAU5000091.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     SLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(7492..8604)
                     /locus_tag="JD016_10635"
     CDS             complement(7492..8604)
                     /locus_tag="JD016_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid racemase"
                     /protein_id="HAU5000092.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            8902..9168
                     /locus_tag="JD016_10640"
     CDS             8902..9168
                     /locus_tag="JD016_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000093.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            9413..11140
                     /locus_tag="JD016_10645"
     CDS             9413..11140
                     /locus_tag="JD016_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000094.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            11140..12930
                     /locus_tag="JD016_10650"
     CDS             11140..12930
                     /locus_tag="JD016_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000095.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(13092..13400)
                     /locus_tag="JD016_10655"
     CDS             complement(13092..13400)
                     /locus_tag="JD016_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000096.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
BASE COUNT         5104 a         1567 c         2237 g         4676 t
ORIGIN      
        1 gatatagtca aacatattat aattatagaa gggaaattta agatggattt taaaaattta
       61 cagtatgaaa gtttgtcata tattatttgt aacttgcaaa aaaactttaa actttattgt
      121 gaaaaatgtt taaaaccata taaacttacg aatggtctat atttctattt aatttatatt
      181 aataaaaata ggaattgttc tttgaatgat gtttctacag aatttgaagt tgataaagct
      241 catacaacaa gaactatatc taggttagaa caagatggat acatagaaaa aatccaaaac
      301 cctaatgata gtagagcttt tcagttgaga gtaacagata agggtgagga agtattaggg
      361 gatataaaaa acatattttc aaagtgggat aatcacataa aaaaagagtt ctcggataca
      421 gaatataaag aactggtaaa gaatttacat gtggtaaagg atataaaaac agctgtggag
      481 gaagaatagg atgtattgta atttattaaa accaattaaa attggaaatt tggaattaaa
      541 aaatagagta tcttttgcac ctacaagcat gggtcttaaa ttggaagaaa aaattaaaaa
      601 gttttctgat attgctaagt caggagtagc tctgattact ctaggagatg tatctattag
      661 acctagtttt cataaggtag ctataagcct atcggatgaa gatggagtca tgaaatacaa
      721 gaaaattgta gatgaaatac acaattctgg tgcaaaggta tcagcacaac tattttgttc
      781 agattatgat gttaatttaa ttaaagatac tatgaagatg ggaattactt cacatgatga
      841 gataaaaaaa ataatgaatg acggagtaaa agattacata acaaatatgc ccaaagaaga
      901 aattaaaaat attataaatt tatttaaagt aacagcattg aatgcaaaaa aagcaggttt
      961 tgacatgatt caaattcatg gtgataggct tgtaggaagt ttttcatcaa gtatatttaa
     1021 taatagaaat gatgaatatg gtggaacgtg tgataataga agtagatttg caagcgaaat
     1081 aattagtagt atccgagatg aagttaagga cattccaata gactataaat ttgcaataag
     1141 acaagaaaat cctcattatg gaaatgcagg agttttactt tcggaagttg agtattttgt
     1201 taaaaaattt gaatccctag gtgtaaacag ttttcatgta acacttgcaa atcattcaaa
     1261 attagaagat actataccaa ccaataatca tccttacttt aaagatgaag gctgtttttt
     1321 atatttagct gatgaggtaa agaaacacac aaatcttcca gtatgtgggg ttggaaaatt
     1381 gtctagccca gattttatag aaagtattat ttcaaataat agagtagata tggtttctat
     1441 gtcaagacaa ttactagcag atagtaattg gttacagaaa gtaaaagatg gaagagtaga
     1501 tgagattaaa aagtgctgtt attgtaataa gaagtgtgca gatgctttac aaacacgctc
     1561 tcagtttgga tgtatactag attagaatat ccttataaaa ataaataatt tgttaaggtt
     1621 aatatatttt tggtgattta gataagtatt tagataaaga ggaggagaca tcatgaaaag
     1681 tttagtttta tattcaagtt taactggaaa tactaaaaag attgcttata ctatatatga
     1741 tgaaattcaa gaagaaaaag acattaaaga tgtaaatgaa ttagttgatt atagtattga
     1801 ctatgaaaat tatgatatag tattcttagg atattgggtg gacaagggaa tttgtgataa
     1861 aaattcaaaa caagtgcttg aaaatattca caataaaaag atagcactat ttggaacaat
     1921 gggagcaagt gaaaaaggaa gttatggagc tagtataata gaaaagatag aatcaattat
     1981 cccaaaagat aatgaaatat taggttcatt catatgtcaa ggtaagatag cagaaggatt
     2041 gaaagcaaaa tataaagaga tgttaaaact ttctcctgac aacgaacata ttcgccaaca
     2101 attaaataac catgaggaat ctcaaagtca tccagatgaa caagaaatat atgaagccag
     2161 tatgtttgca aaaaatatga tgattaaagc tagtattgtg tagtagttaa acaataagtt
     2221 catataagat atagaataaa taatttagta aaaaagtata gcaaataatt tgctatgctt
     2281 tttttatgaa atcaaaaaaa taataaaaat acttgactgt aaaccttgtt tatacattat
     2341 tttatagaaa ataagatagt tttaaatata aaaaaagata tattattgaa aggagatatt
     2401 attgtatgaa aacttataaa agataattag tttgatttta agatatacta aatatacaat
     2461 tttatagagt gaaaatattt aagactatat tatagttata aattaataga atagagaggt
     2521 agtgtttaaa ttgcaataac agtatttcca aagttgccac ctcaaattcc tatccaatgg
     2581 aataatggtg tagctaatta tgtagataaa aaatttattt atgcctaccc tgtcatttgt
     2641 gttgtattca gaatcctttt acgtccattt atatggagat ggttacaaat acatgctttt
     2701 tatagtgata ctattgcaga ttatttaacg aattttatgt gttttgtagc gttgtctata
     2761 gaaatattct ctatactttt tatttacaat attgccagac atgttacaat aattattttt
     2821 atagatatac ttgtatttat tgctttactg ataataggtg tgtataaatt atttaaaaaa
     2881 tacaattaat ttttaaaaat aagtaataag tgatagtgta aaaagtagcc actgcaaatt
     2941 atggattgta gtggctattt tattatttgt atatgaaatt attttgtttt tctaataagt
     3001 tacgatatat tatggtttat ttcactgata aaaggtttag gtattttaaa gtaaaaagtt
     3061 gtatattcta ataaaattta aacattttga ttctttaaaa ttagagaaaa tacattgctt
     3121 ttattaaaaa attaaatatt aagaatttaa tttctgcatc ttatttagaa aagataagaa
     3181 atcaacttta tatatttttt ttggccttcc tctttgatta tcgagttttg tatttacaat
     3241 agttgcaatt cctgctttat ataaaatact caatattcta ttagcacttc taagagttat
     3301 gttcatatag tctgctaata tttctgctga cacattattt gaatttctat cattaatcat
     3361 acacatgatt ttagatacat ttgtattaga tagaggtata aaagaagcaa ctttattggc
     3421 tattgagcta tcttcaatat tattagaatt tttatttgaa tataaaggac ctattgtatc
     3481 attagtatca tttactataa aagtacagtt tccattcaaa gaagcagatt taccattagc
     3541 ttgacgagca ttttgctctg catgaatatt tgtatttcca atgccccaac ctatatttaa
     3601 gctatattta atttctttta aatcttctga aataaaaaaa tctgtatagt tattggtcat
     3661 attgtttaac atttctttag tagtgtgtat ctctattttc atatcatttt ttttgacctg
     3721 aactccataa aatccatgtc ttgatagaga atttaatata gtgttatata tttgattttg
     3781 tatctcatca tttttaaata tcaaatcttc atttatgtac tctgaactat ttactgtaac
     3841 tatacaagtt gcagatttat ttttgtctaa gttttgtaat gttatttcat tcaccagaga
     3901 tttaaatgta ttcaggattg attctgtaga tggaaccatg taaatacaat tatatccaag
     3961 cttattaagt tcacaactaa tgatgtaatt tctagtaaaa gaaaggttaa tcttattatc
     4021 tcgcaataag tctaaatgta tttccaataa tttatcattt aattttttaa agtcatatac
     4081 aggttgaggg atattaaaat ctttaattgt atatggacat ttattagagt ctattaatga
     4141 atatatatca taataattgt tgtcctctct taaaaaatcc ataaatgatt ttgtaaagtc
     4201 aatatctttt tggctattta ataatttgaa taagtattta taaaaatctc tttcatcaag
     4261 ctttaaaaaa tttaatggaa cttttatgtc ttttatattt attataagtt cactatatgg
     4321 aactatacca ctgcatacaa atccatcaaa attatctact tgctcatagt atatattagg
     4381 taaattatta aaaggattct cccctttgag ctcaataaat gtaatttcac aagtatcatt
     4441 taattcttta agagcttgtt taaacttatc tgacataaaa taagatgttg tcaaaaaacc
     4501 aattttatat ttcataaaac ctcctttaaa atttcgagag tattgtaact aatcaatcaa
     4561 tttataacct cattttataa tagtagataa aacgcgtcaa tttattttac aaaaggaaaa
     4621 ttgaaagaaa aatcagaaaa tataaaaaag actgaaaaat atgttgactg tcaagaataa
     4681 ttgtgctatt ttgtaattaa ggactagtct tttaagattt cctaaaataa acttaggttt
     4741 taggaaagaa atacatttta tttgaaaata aaggagaaga ttatgaatac atatttaaat
     4801 gaaatttcta agtttgttga tgagaataga gaagaaatag tttcattatg gaaagagatt
     4861 gttaatatgg aaagctatac acattgtaaa gaaagcgtaa ataaattggc taagagatta
     4921 aagtttgagt ttgaaaaaga aggtttagat tgcgagttag tagatgttgg agataatggt
     4981 agtacattga taggtacact tggttctaat ataaataaaa agcctataat tttttcaggt
     5041 cacatggata ctgtctttga aacaggtaca tttggagaga atccatttaa aataatagaa
     5101 ggaaaagcat atggcccagg tgttttagat atgaagggtg gtataataat ttccttatat
     5161 gttattaaag cattaaataa aataggatac aaagaaagac caataaaaat agtattttct
     5221 ggagatgaag agataggaca taaggattca acaggtgcag atgttatatt gagggaagca
     5281 aaaggtggtc tttgtgcttt taatatggaa actggattag tagataatag tttgtgtgta
     5341 ggtagaaaag gaagaatagg atgtaatata catgtaaaag gtgtagaaac acacgcaggt
     5401 aatgattttg aaggtggaag aaatgctata gaagaaatgg ctaataagat actacgcata
     5461 caaaaattga ctaatttaga tgttggaact acagtaagtg taagtgtgat taagggtgga
     5521 agagtggaaa attctattcc agaagattgt agtataaaag tagaccttag atttgaaaaa
     5581 gtagaagaaa tggagaatgt taaagaacaa gttagagaga tatgtaaaga aacttacata
     5641 gaaggaactt ctacagaagt taattttata agtgaaatga tgccatttga aactacagaa
     5701 gatgttatga gatttcatac atttgtaaat gaagtgtcaa aggaaaatgg atttggagaa
     5761 ttaaatgcta agagacttgg aggtagttca gatgcaagtt atttaactat tgcaaacgtt
     5821 cctacaatct gttcatttgg tgtaagagga gaatggaacc acacatcaag agaatatgca
     5881 gtagtagatt ctatgtttga gagggtgaaa ttaatttcta cagttatttt aaacttagat
     5941 aaatttgagg tataaagggg gattttatat ggatgtaaca gctggagata ataaggtaac
     6001 atggaaaggg tatttatctt tatttgtatt gatagtatta ttttcaggag tgtttaaaga
     6061 tagtcaaggc ttccttaaag cattagattt tgctaatctg acaggttcat ttggtactat
     6121 tgcagatggt gtagacttta tgggtaaagg aggaacggga gcaaaagatg gtttcttatt
     6181 tgctctgact ttgattccaa ctgtctgttt tgctgtaggt cttatagatg tagttgaatc
     6241 tatgggagga atgaaagcag catcaaagat atttaatcca ttacttagac ctctgcttgg
     6301 tatacctgga atagcaggaa tagcatttgt atcttcattt actagttcag atgtagcctc
     6361 aataatgacg aaagaattat ttgaaagtgg ggaaataaca gatgatgaaa gaactatatt
     6421 tgtagcatat caatatgctg gttcagctgt tatactgaac acaataaata ctcaagctcc
     6481 acttttacca atagcattat tggcattagg tccaataatc cttatagaga tattctgtaa
     6541 aatacttggt gctaatctag ttagattaat cataggtgta agaaataaaa agaataaggc
     6601 taaggaggct gtgtagtatg agtcaacaaa atactggaaa tgcaattgta aatagtaaac
     6661 ctaagaaaac tgctgttgag atatttatgg ggggagcaaa aaaaggtttt tacataggtg
     6721 ttgaacagat attaccagcc atgataatgg gttatgtaat agtgcagttt ttacaattaa
     6781 ctggtttagt tgatatttta ggaaatattt ttggaccagt aatgggtatt tttggactac
     6841 ctggggaatc tgttgtagtt ttgatagcag cattctttgc taaggcagca ggagcagcta
     6901 cagctgcaaa cttatttgca caaagtctaa tcactgcacc acaagcaacc atattaatta
     6961 tgccatgtat gttaatgggt actctagttg gtcattatgc cagaatagtg cttgtagcaa
     7021 atgccagtac taagtataga atgttactgt tggcagttcc tatatttgat gcaatagtgg
     7081 ggatgttaat agtaagagtt ttactcactg ttatgggatt aatgtaaata acataaaatt
     7141 ataacctaac tataaatgat atataagaat taatatatac aaggattaat gaatagtaat
     7201 tgatatatag taataaacta agatagtaat taatgtgtat attaattaaa aaaaagagag
     7261 tatctcaaag tgaacttttt agttcatgag atactctttt tgtatgaaat caagtatatt
     7321 ttcataattt caacaatgaa agagaggctt atctctattt tgagacagcc tcttctttta
     7381 aatttatatt tctagaattt atgtttttta tttatccaac attaacatta gttattaaca
     7441 ataattttaa gaaaaatata ctttcatgtt aatatcagaa taaatctgtt tttaaaggat
     7501 gatttgtttt gaattttcac gaatatctac ttctatgact ttttctattg tatctcctct
     7561 aaaacctaat aaattacttg tcaagtttgc agtggaacaa gaatgattag gtataattct
     7621 tattctatca ccaattttta tatcagtttc accatttact ttaatttttc caacttcttc
     7681 tgaaagtgaa tcaatagtaa gttctggatg tccacatacc ctaccataac caacaatact
     7741 actatttcca tgtgcacctt tatcaagtcc aaggcactta cttccacaat caattaaata
     7801 gtatccactg ctattttttg agattacagt gcacacaaca gtaagagcac acatatcttc
     7861 agtagcacaa cctaaaccta tctgtatagc atcatagtat acatagtttc caggtcttac
     7921 agcagttata gttttagaag cagcttcaga ttcaactgtt ggagtactac cagaagcgac
     7981 aatttctata ttaaatccat tatcagttag agctttgtat gctttctcca tagaagaatc
     8041 ttcttgaaca caaacatctg gaacaccttc tggagaacta caaccataca cttgacctgg
     8101 atgcgaactt ataccaacaa tctttatgtt ttctagctta gaaatttcag tagcaagttc
     8161 aaccactttc tctggttcaa ccccaaaacg gtgtaagcca caatctacaa taataagtag
     8221 cttgcactct atttttgatt tagaaaaagc ttcactataa gccttagcac attctatatt
     8281 atctgtagca cagattaaat ttatttttga tgccatagtt acaattctat ttatatttac
     8341 ttctcccatg taaggataag ccaacataat atcttcaaaa cctgctttta tcagagcttc
     8401 accttcatca agtgttccaa ctagaaaacc actagcacct gcattttttt gaagtctcgc
     8461 tatgtaagta cttttatgtg tcttaagcat aggccaaagt tgtttattat tatttgtaca
     8521 aatttgttga attttatcta tattcttttc taactggtct aaatctagca aaaaatttgg
     8581 agtagcgact tctgaaattt tcatgttatt taccaccttt caacaaaagt aaattaacta
     8641 taagtattat aattattata ttagtaatat cggctttata atattaatag cttataatta
     8701 tattatagaa ctagcgttaa cagagagcaa tatttatttg taataattag aattttcaat
     8761 aagttagtgt agttttaaat tatttatata atatattttt aattttatgc aaagtttatt
     8821 taaattagtt tgaggattta ttaatagggt attattgaat gagtaaaaaa gtgatgttta
     8881 catagaataa tcacactata aattaattta gaacctaaaa aattaattga gcaaattgga
     8941 aaagagaagg ataaaaaaga tatttggata gttggtggag gcgatatagt aaaattattt
     9001 atgaaagata acttaattga tgattattat atatatatat tgccaataat attggggaga
     9061 gggatttcac tatttaagtc tggtatagat aagactaatt tgaattttaa gaaagctagt
     9121 aatattggag aacttgtaaa attggaatac agtaaaaaac aaaaataaat tacatgccaa
     9181 tttgttacaa tgttatgaaa tgaagcattt aaaagacttt tggctatatg aattgacatt
     9241 acttgtcaaa tatgttaaca tataagcgac ttgtcggagg gtgtattaaa aagaatatgc
     9301 tggagaagag agtgcgttaa ctcttttctc ctttttttat aaaattaagc atttataaat
     9361 ttagtattat tacatgatta cactatctca aaaattaaga aaggagaaaa atatgaaatt
     9421 aattttaagt tatttaaaga attataagtt attaatagta ttaaacatac tggctatatt
     9481 tagttttgca ttagtcgagt tggggattcc aactataata gctaaaataa ttgacaatgg
     9541 tattgcaaat cagaatatag cttacataaa acaaatggga atagtgattg ttattatttc
     9601 tattataggg gttgtaggta gtatattgtt gggttattgt tctgctaagg tatcaacaag
     9661 tgtaaccaga gatataagaa atgacatatt tgagaaatca caagaatttt cacatactga
     9721 atacaataaa tttggaattt cttcaatgat tacaagaaca acgaatgatg catttcaaat
     9781 tcaacaattt gttaatatac tactaaggac tgccttatta actccagtta tgttcattat
     9841 aagtataata atgacaatta gaaccagtgt ggaactttct atagtattgg ctatatcagt
     9901 accttttata attattggag tagcaataat agcaaaagta tctcaggcaa tatcttcaaa
     9961 acaacaaaaa ggactagata aattaaacct tatatcgaga gaaaatttaa ctggaataag
    10021 agtaattaga gcttttggaa atgatgatta tgaaacagag cgatttgaaa aaacaaatac
    10081 atattatgct aatgtttcta agaaactatt taagcttatg tcaattactc aaccagcatt
    10141 ctttttactg ttaaatatag ctgttttggc tgtattttgg atttcaagtg agaagataaa
    10201 tattggagag cttcaagttg gacagttggt tgcatttttg gaatatcttt tccatgccat
    10261 gttttcaatt atgctttttt caatggtatt tattatgtat ccaagagcag aggtatctgc
    10321 aaatagaata aaagaactat taaatgaaga accattgata aaaaatcctg aaaatggaat
    10381 taaggatact gaaaataagg gtatcataga atttgataat gtaactttta cctatccaga
    10441 tggagaagct tctgtactta aggatatttc atttactgca aaaacaggtg aaacagtcgc
    10501 ttttatagga agtactggta gtgggaaaag tactctaatt aacttgatac ctagatttta
    10561 tgatgtaaca gaaggtagca ttaagataaa tggagtagat ataagagaat atgatttaaa
    10621 agctttaaga aaaaagatag gattcatacc tcaaaaatca ctgcttttta ctggtagcat
    10681 agcaaataat attagatttg ggaaacataa ggcaggagag tcagaattag aatattccgc
    10741 aaaagttgca caagcatatg agtttatatc aaaaaaacct agaaagtttg atgaattgat
    10801 tagtgaaggt ggagcaaatg tatcaggagg tcaaaaacaa agactttcta ttgcaagggc
    10861 tattataaga agacctgaaa tatacatttt tgatgacagt ttttctgcac ttgactttaa
    10921 aactgatgca atacttcgtg ctaaattgaa gaaggaaaca aaagatgcaa ttgtattgat
    10981 tgttgcccag agaattagtt ctattattga tgctgataag attatagtac tcaatgaagg
    11041 acaagttgta ggaatgggaa ctcataaaga attattaaaa aactgtgaaa tatactatga
    11101 aattgcaaca tctcagttaa aaaaggagga attagaataa tgaaaaagtc taaaggtttt
    11161 aagaaaacaa ttggtagact tcttccattt gtaaagaaat ataaattttc ctttattata
    11221 gctattatat gtataatttc agcagctact atgaatgcac ttgcacctaa aactgaagga
    11281 ttgataataa cacaattgac taaagatgta attagtatcg caaaaggagt accaggggct
    11341 tccgtaaatt ttgattatgt aacaaaaata ttggtgattt tagcatgtat atattttgcc
    11401 aatgcaattt ttacgtatac ttcaagtttt ctacttacga atgctattca aaacacaatg
    11461 agagatttaa gaaatgaagt cgaaaataaa ataagaagat tgcctattag ttattttgat
    11521 agtaatagtt ttggagatgt attaagtcgt atatcaaatg atgtagatac aatatcaaat
    11581 gcattgcagc agagttttat gcaaattgta aactcaatct tagtcataat tttggcactt
    11641 tcaatgatgt ttacaattaa tatatatatg gctcttatag ctctttttat aattccaatt
    11701 agttattttg tatctaaatt tgtagtaaaa aaatctcaaa gtagattttc attgcaacaa
    11761 aatgcacttg gaaaattaaa tgggaaagta caagaaatgt atactggatt taatgaaata
    11821 aaactatatg gtaaagaaga agattctata aaagaattta aaaaagtaaa tcaagaactt
    11881 tgtgaaaatg gatttaaggc acaatttata tcaagtatga tgaatcctat ggtttcatta
    11941 gtaacttact ttggaattgc agctgtggca gttgtcggtt ctatatatgc agtaagtgga
    12001 ggaataacag taggaaattt acaagcattt gtgcgttata tatggcagat aaatcagcca
    12061 ctttcacaaa tgactcagtt atctacagta atacaatctt cttttgcagc tatagaacgt
    12121 gtatttgaaa ttttagacga agaagaggaa atacctgatg ttgaaaatcc agttaagata
    12181 gaaaatgtaa aaggaaatgt tacatttgag catgttaatt ttggatatgg agaaaattca
    12241 actttaatag aagatttaaa tgcagaagta aaaagtggtc agatggtagc tattgttgga
    12301 cctactggag caggtaaaac aacattaata aacttattga tgaggtttta tgatgtaaag
    12361 aaaggtgcta ttaaaattga tggagtagat ataagagata tgaaacgtaa agatttgcgt
    12421 tcaatgtttg gtatggtgct tcaagataca tggttattta atggaaccat ttttgaaaat
    12481 atagaatatg gtagatttgg ggctactaaa gaagaaatta tacaagcagc aaaagtagca
    12541 aatgttcatc actttataac cactcttcca gatggataca atatgttttt aaatgaagaa
    12601 gcatctaata tatctctagg ggaaaaacag cttttaacca tagctagagc ttttatatca
    12661 gacccttcaa tattgatact tgatgaagct acgagttctg ttgatactag gctggaactt
    12721 atgcttcaaa aagcaatgag aaatcttatg aatggtagaa ctagttttgt tatagcacat
    12781 agactttcta caattcgcaa tgctgacttg attttagtta tgaataatgg aagtattata
    12841 gagcagggga atcatgatga attaatggaa aagggtggat tttatgagaa gctttataat
    12901 agccaatttg cagacaaaga atcagaatag ataacataaa cttttgtata aaattttgta
    12961 tcaaattcaa atattaatca tataattaaa aagagtatca gtaagttgtt tttttataga
    13021 tttactgata ctctttgatt ttaagactaa aggtacttat gattggtact tataattcac
    13081 aactagtctt gttacatctg ataaccagaa ttacagtact tttctttttt acctttttgt
    13141 tctctaacat ataacaattc gcttaactta tcaatgtaat aaccatcatc acttatgcga
    13201 attaaactag tcagataata tccatctttg cttctaggaa tctttttatt tattgctaaa
    13261 tcaatagctg tctttgctgc ttcaaaatgc tttaaatgtg tatgaccttc ttcaaaactc
    13321 tttctagtat tataaactac ataacctttt ttaacttgaa aaactataaa ttctttcttt
    13381 tcatatattt tattaaacat tcaatcagcc cttctcaaga tagttttata gcataacatt
    13441 agaagcttct aaaaaatttt acaaagaaca taaatatttt ataaattata aatctaaaac
    13501 tataatttat aaagataact ttctgattat ttatatgata attcatatag attaaaagta
    13561 aattatcttt aggttaaaca aaag
//
LOCUS       DACXMZ010000093        13449 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.094, whole genome shotgun sequence.
ACCESSION   DACXMZ010000093 DACXMZ010000000
VERSION     DACXMZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.094"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            135..1448
                     /locus_tag="JD016_10660"
     CDS             135..1448
                     /locus_tag="JD016_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAU5000097.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            1620..2795
                     /locus_tag="JD016_10665"
     CDS             1620..2795
                     /locus_tag="JD016_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAU5000098.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            2898..3917
                     /gene="hrcA"
                     /locus_tag="JD016_10670"
     CDS             2898..3917
                     /gene="hrcA"
                     /locus_tag="JD016_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HAU5000099.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            3947..4567
                     /gene="grpE"
                     /locus_tag="JD016_10675"
     CDS             3947..4567
                     /gene="grpE"
                     /locus_tag="JD016_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAU5000100.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            4659..6506
                     /gene="dnaK"
                     /locus_tag="JD016_10680"
     CDS             4659..6506
                     /gene="dnaK"
                     /locus_tag="JD016_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAU5000101.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            6717..7871
                     /gene="dnaJ"
                     /locus_tag="JD016_10685"
     CDS             6717..7871
                     /gene="dnaJ"
                     /locus_tag="JD016_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAU5000102.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            8083..8199
                     /locus_tag="JD016_10690"
     CDS             8083..8199
                     /locus_tag="JD016_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000103.1"
                     /translation="MGTDVALPNDEENFIVIYKIKLDTFSARLSTGKTHKLG"
     gene            8837..9772
                     /locus_tag="JD016_10695"
     CDS             8837..9772
                     /locus_tag="JD016_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAU5000104.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            9808..11181
                     /locus_tag="JD016_10700"
     CDS             9808..11181
                     /locus_tag="JD016_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAU5000105.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(11369..12136)
                     /locus_tag="JD016_10705"
     CDS             complement(11369..12136)
                     /locus_tag="JD016_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HAU5000106.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(12166..13275)
                     /gene="ugpC"
                     /locus_tag="JD016_10710"
     CDS             complement(12166..13275)
                     /gene="ugpC"
                     /locus_tag="JD016_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAU5000107.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNNIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT         5175 a         1521 c         2358 g         4395 t
ORIGIN      
        1 ttgtatgatt tttaaatgca taggcattta ctctaggact atagactaat ataaaaaaaa
       61 actttgggaa tactaaaacc ataaatttat aaaattaata ttttatatac tgtaaaacag
      121 tgaggaggga cagtatgcat actcaactac aaaagactat tggtctttca gcagctcttt
      181 ctactgttgt aggtatggtt ataggttcgg gagttttttt taagccacaa gcaatataca
      241 ctacaactaa tggagctcca ggtctaggta ttatagcgtg gcttttaggt ggttttataa
      301 caataactgc tgggcttaca gctacagaaa tttcagctgc tataccaaaa actggtggta
      361 tgatgattta tatcgaagaa atatatggag aaaagttagg ttttttaact ggatggatgc
      421 aaactgtatt atttttccca ggtacatcag cggcattagg agtaatattt gctcaacaag
      481 catctgaact tttaggaatg agcccaaata atatggcaaa tgtacttcct atagcaattg
      541 gagttatact atttttagcg ttattaaata taataggttc atccttagga ggaaaagttc
      601 agacagtagc cactattgga aagatgatac ctttaatttt aataatagtc tttggattta
      661 ttaaaggtca aagtagtgaa gtattaaatc catttgtagg agatggtgta aatgcatcaa
      721 atgcacttgg acaagtatta atagctactt tgtttgcata tgatggttgg ataaatgttg
      781 gagctatatc aggagaaatg aagtctccag agaaagattt accaagagca atagttggtg
      841 gtttatcatt agttatggca gtttatatta taataaatat agcttatctt tgggtagtac
      901 cagcaagtga attagcaaca gttacttccc cagctacatt agtagcaaca agattgtttg
      961 gaaatatagg tggaaaagta ataactgtgg gtatattaat atcagtattt gggacattga
     1021 atggatattt attaacaggt tcaaggatac cttatacatt agctgaaatg ggaactttac
     1081 cagcatctaa aactctttta aaagtaaatt ctggaggttc tcctgtaaat tcaatactac
     1141 ttataactgt gttagcctgt gtatatgcat tatctggtca atttaatctt ttgacagact
     1201 taacaatatt ttcaatatgg gtattttatg tattgacttt tataggtgtt atgaggctta
     1261 gaagacaaaa gccagattta cacagaccat ataaagtccc tttatatcca ataataccaa
     1321 ttatagctat attaggtgga ctgtttgtaa taataaatca aatattgact tcaacagtca
     1381 tatctttagg cggaatattt ataacactat taggtttacc agtttattat tatatgaaaa
     1441 aaagataact acttaattta agttagataa atttgggtaa aggtgaaaat taacttttat
     1501 ccaaattttt tatactgtta tacaatataa gtattatata ttgcttgatg tgctattttg
     1561 tttgatttat aatttagtaa ttgatgtttt aagataattt aagaaatgag gagtgaaaga
     1621 tgctaggact ttatgtacat attccatttt gtgtgaaaaa atgtaagtat tgtgatttta
     1681 attcttataa aatggacata gattcaaaaa aaagatatat agaagactta aaaatagaaa
     1741 tggaattata tagtaataaa ttatataaag ataataaata taaaaataaa gaatgctgta
     1801 gtctaaataa aaatgacaaa ataacgagta tatttgtagg gggaggtaca cctagtatac
     1861 taacctctga tgaaataaga gaagtattta taagtataaa agaaatgttt gatatagatg
     1921 agaatgcaga aataactata gagtgtaatc ctgggacatt aacattagaa aaattaaaaa
     1981 ctatgaaaga gattggcata aatagactta gtatagggct acaagctatt caagaaaagc
     2041 atttgaactt tataggaaga atacatactt atgaagaatt tgagaaaaac tataaagatg
     2101 cattaagtgt aggttttaaa aatattaata tagatttaat gtacagtctt ccaaatcaaa
     2161 ctctatgtga ttggaaggaa actcttgaaa aagttgtaca cttaaatcca acacatatat
     2221 cagcttattc ccttatacta gaagaaggaa ctgaactata caatatgtat gaaagtaata
     2281 aatttgaact tatagatgaa aatgtagata tagaaatgta tgagtataca ataaactact
     2341 taaagtcaaa aggatataat caatatgaaa tatcaaacta ctcaaaagaa ggctataatt
     2401 gtgagcataa tattttatat tgggaatgtg aacattatat agggattgga gcaggagctt
     2461 ctggctatat taatgagaac agatacaata atgtagaatc tttagaagat tatcatttaa
     2521 gtttggtaaa aagagaaaaa ccaatacaag aaaatgaaat tctttctgag aaagatatga
     2581 tagaagaaaa gatatttatg gggcttagaa tgaataaagg cataaaattt gaagatttta
     2641 aaaaaaaatt tggaatagat tttagagaga agtataataa gcaaatagaa atgctattag
     2701 ctagaaagct tataaatcag agctttgaag gtatacaatt aactcaaaaa ggaagagaaa
     2761 tatctaatag tgtatttata gaatttatgg aataattttt aaggtgttga caaaataaat
     2821 ttataatggt atataatata tataatcttt ttagcactcg aacttagtga gtgctaacag
     2881 ctaggaggta taataatatg gaattaaatg aaagaaaatt aaatatcctc aaagctatag
     2941 ttaaagatta tatagaaaca gctgaagcta taggttctag aacaatatct aaaagacatg
     3001 atttaggtgt tagtgctgct accataagaa atgaaatggc tgaccttgaa gaattagggt
     3061 atttaattca gcctcatact tctgcgggta gagttccatc tgaaaaaggc tataagttat
     3121 atgtaaactc tcttatgagt aaaagcgaat tggatgataa tgataagatt cttattgaac
     3181 aatgtatgaa tcacaatata aatcatataa aagaattgat tcatgaaact tctaagctat
     3241 tatcgcaact aacaaactat acaactgtag cagttactaa gagtttaata aatcaaagtg
     3301 ttataaagca catacagttg gttgctatga atgacaataa tattttactt atagttgtaa
     3361 cagataaagg ggacttaaaa aaagctaacc ttacaactaa tgtatattta gatcaatcta
     3421 agttaaattt gatttctgat aatcttacaa ggaagctctt aggtaaaagt ataacagatt
     3481 tggatgataa tctaattgct tttattaaat acgaaataag tgagtattct gggcttatag
     3541 atgagctttt aaatgctttg aactcaaata tgaaagaaga agatttttct ctatcattaa
     3601 atggagctac taacatattt agttatccag aatttaatga tgtattaaaa gcaaagtcat
     3661 ttttaaatat gttggaaaaa aaggaaacaa tagctgacat aattaaatca aagggaatac
     3721 aaaaagacaa tctaaatata ataattggta gtgataatga ttgtgaactg gcacaggatt
     3781 gtagtatagt tacagcaact tataatgtag atagagactt ggttgggcga ataagcttta
     3841 taggccctac cagaatggat tatgctagaa tttactctat aataaattat atgagtttat
     3901 taattaatag aaagtaaaga aattttaaat tgcgaggtgt aaagtcttgg ataaaaaaaa
     3961 tgagcaacag gaagtaagag aagaaaatga tacttctatt aaccaagaat cggaaactca
     4021 agtagagtta gaagaggaag ttgttaatga agaatgtgaa acatcaagtg aaaaaactga
     4081 tgaaaaagaa gttgatgatg aaaatgtaac agatataaat tctaagttag cagaaaaaaa
     4141 attgcaggat gagttggatg aattaaatga taaatatcaa agacttcaag ccgaatatgc
     4201 aaactacaga agaagaactc aacaagagaa agagacaata ggcgtatttg caaatgaaaa
     4261 aataataact gaattgatac cagttataga tagtatggaa agagccttag atgcatgtga
     4321 agataaggaa gatactatgt ataagggtat aagccttgta cataaacaat taatagacac
     4381 tttagtaaaa tttggagtag aagaaataga agcagaatct aaggaatttg accctaatct
     4441 acatctagca gttatgcaag aaagtgtaga tggagttgaa gcaaatcaaa tagtaatggt
     4501 acttcaaaaa ggatataagc taggaactaa agttgtaaga ccttcaatgg ttaaagtttc
     4561 ttgctaagta taaattaaag gttaaattat ttaacctaat ataaaatatt aaaataagta
     4621 aaacttaatt atataattac tcgaggagga ttttagtcat gggaaaaata ataggaatag
     4681 atttaggaac aacaaattca tgtgttgcag tgttagaagg tggagaagca caaataatag
     4741 caaatagtga aggtatgaga actactccat cagtagtagc atttacaaaa gatggtgaaa
     4801 gaatagttgg agaacctgca aaaagacaag cagttacaaa tgcagataaa acaataactt
     4861 ctataaaaac tcatatggga actgattata aagtaaatat agatggaaaa tcatatactc
     4921 cacaagaaat atcagcaata atattacaaa aattaaaatc agatgcagaa agttacttag
     4981 gacaaacagt aacagaagca gttataacag taccagctta ctttacagat gctcaaagac
     5041 aagcaactaa agatgctggt agaatagcag ggcttgatgt taagagaata ataaatgagc
     5101 caacagcagc agctcttgct tatggtatgg ataaattaga ccaagaaaag aaaatattag
     5161 tatttgactt aggtggagga acttttgacg tatctatact tgaaatagga gatggaactt
     5221 ttgaagtttt agccacagct ggtaacaata gattaggtgg agatgacttt gaccaaatag
     5281 taatagatta tttagcagag gaatttaaaa aagctgaagg tgtagattta agaaatgata
     5341 aaatggctct tcaaagatta aaagaagcag cagaaaaggc taagaaagaa ttatcatcaa
     5401 caatgagttc aaacataaac ttacctttca taactgctac agcagaagga ccaaaacact
     5461 taaatataga tttatcaaga gctaaatttg aagaattaac tagaggttta gttgaaaaaa
     5521 ctatggaacc aactaagaca gctttacaag atgcaggact ttctacaggt gatatagatg
     5581 atgtattatt agtaggtggt tctacaagaa taccagcagt acaagaagct gttaagaagt
     5641 ttataggaaa agaacctcat aaaggtataa acccagatga gtgtgttgca gcaggtgctt
     5701 ctatacaagc tggagtttta gcaggggatg ttaaagactt attattacta gatgttactc
     5761 cattatctct tggtatagaa actatgggta atgtaatgac taagataata gaaagaaata
     5821 ctacaatacc aactaagaag tctcaaatat tctcaactgc tgctgataac caaacagctg
     5881 ttgatataca tgttcttcaa ggtgaaagaa gtatggctta tgacaataca actttaggaa
     5941 gattccaatt aacagatata ccaccagcac aaagaggaat acctcaaata gaagttactt
     6001 ttgatataga tgcaaatggt atagttaatg tttcagctaa agatttaggt acaggaaaag
     6061 agcaaaaaat aactataaca tcaaacacta atttatcaga agcagaaata gagcaaaaga
     6121 taaaagaagc agaaatgaat gcagaagctg acaaacaaaa gaaagaaaaa atagaagcat
     6181 tcaatcaagc tgaatctact atatatcaaa ctgaaaaaac tttaaatgaa cttggagata
     6241 aaataagttc aggtgaaaaa gaggatatag aaaaagctat agcagactta aaagctgtaa
     6301 aagataatca agatgctaca gcagaagaac ttaagaaagc tacagatgaa gtaatgacta
     6361 agttccaaaa agtatctcaa gaaatgtacc aaaaagcagc tcaagaacaa caagcggcac
     6421 aaggtgcaga acaagcacaa gacaatggac ctaaagatga caatgtagta gatgctgact
     6481 ttaaagaagt agatgaagat aaataagcta tataatatgt aacactaaat gataattgga
     6541 acatatatga tttgtgaaaa tcatatagat gttaaagata tatggacaat atacatcata
     6601 aagaatataa tatatcatag attatataac ttaaagtaga atataagaat taatgatagc
     6661 ttgtagttta ttattctaca agctatcgtt ttgtaaaatg aaggtggtgt caatttttga
     6721 gtacaaaaag ggattattat gaagtcttag gtatcagtaa aggtgcagaa gctcaagaga
     6781 taaaaaaagc ttatagaaaa ttagcaatga agtaccatcc agatagaaat cctggagata
     6841 aagaggcgga agaaaaattt aaagagataa atgaagctta tgaagttcta tctgatgata
     6901 caaagagaaa aacttatgac cagtttggac atgatgggct aaatggtcaa ggcggttttg
     6961 gtggtcaagg aggatttggc ggtcaaggct ttggtggttt tgaagatatg ttcggagata
     7021 tatttggtga catgtttgga ggaagcttcg gaggtggaag agcaagaaga agagggcctc
     7081 aacgtggtgc agatataaga cagagtgtta ctatatcttt tgaagaagct gcatttggta
     7141 aaaagatgtc tataaaagta aatagaagtg aagaatgtga agaatgtaat ggaactggag
     7201 caaaaccagg aacatcaaag aaaacttgtt caacttgtaa tggaactggt caagttagaa
     7261 ctgttcaaag aactccattt ggtaatatag caagttcaag accatgtagt gcttgtaatg
     7321 gaactggaga agtgattgaa tctccatgta gtaaatgtca tggaactgga aatacaagaa
     7381 aagttaagac tatagaagta gatatacctg ctggtataga tgatggtcaa atgataaaac
     7441 tttctggtca aggtgaagta ggagaaaaag gtgctccaag aggagactta tatattgtag
     7501 ttaatgttaa gtcacatcca ctatttacta gagatggaaa tgatatttat tttgaaatgc
     7561 caattacatt tgttcaagca actttaggtg atgagataga agtacctact ttggatggaa
     7621 aagttaaata tagtgtgcca gaggggacac aaactggaac tgtatttaga cttaaagaga
     7681 aaggaatccc tagaattaga ggtaattcaa gaggagacca atatgttaag gttgtagttg
     7741 aaattcctaa aaaattaaac gataagcaaa aagaactatt gagagaattt gcgaaggagt
     7801 gtgggtcgaa cgtccacgaa aagaagaaaa ccttcggaca aaagatagaa gatatgttta
     7861 agaaaaagta gttaaagtag ccaatatata atcatatttt tgaaatctta gttatttata
     7921 agattgtcaa aggagatatt atgattttta tattggctat ttttgtataa aaaataaatg
     7981 tgcataaaat aaaactgaat ataaataatt ttctgaaaaa tacttgtcct aaaatatata
     8041 atatgctata atataatcaa ataaaagaaa ggggtgattc cagtgggaac ggatgtggca
     8101 ctgccaaatg atgaagaaaa ctttattgta atatacaaaa taaaattaga tacttttagt
     8161 gctagattaa gtactggaaa aactcataag ctgggataat tattgtaatt acctagactc
     8221 aaacttttag tagtaaaata gtaatattat atagacaagg tatctaattt ttaaatcttt
     8281 tgtatgaatt aaaattttta taaagggtat atctgattag aatgtaatta gggaaaccta
     8341 gttgtgaatt acttttagta tatacatgtg taaagaaata tgtatatggt attggttatt
     8401 gtgctagatg tgtatgtaaa ggaatatgtg tctagttggg tatatgtaaa gaaatatact
     8461 tataatctat gaaaaatttt tagtataaat tgctaaaaca ttgagattgt tttttataga
     8521 aagttagttt ataccaacta aaaatgtgct taagatttgt ttgatgttta attataaata
     8581 aggttgtaga taaaaaaata agtaaagaca aaggtttaaa atacttgctt taaatatagt
     8641 ttgttttgaa cacttgccaa attaaaataa ttaataaaat agacatcttc tcatagtaaa
     8701 atatactgtg agaggatgtt tttgctttgt tcataaactt ttatgaaaaa tttgcatagt
     8761 gtcacatttt aatatactat atatataata aaatatagat aaaattaaat gattcaaaag
     8821 agaaaggtga aaagagatgt attatatagg tgtagacata ggaggaacag gtatacaagc
     8881 aggagtagtt gataactatg ggaaaataat ttttaggtca gagtgtaaaa cagtaattga
     8941 aaaaggcttt gaaggaatac ttaatgacat aaaaataatg atttataagt tgttggaaga
     9001 taataaactt actatgagtg acataaaatc tattggattt ggtgttccag gttttataaa
     9061 taaagaagga ttagtaactt gtgtaaattt aaaatggaat aaaaaagctt ttaataaaga
     9121 gttaaaaaga agatttccag atgttgaaat tcatggagaa aatgatgcaa cagtagcagc
     9181 acttggagaa gcaaagtttg gaagtatgaa aggtgctaat gttggagtat tatatacttt
     9241 aggaactggt gtaggtggag gaatagtaat taatcaaaaa gtcttttctg gagcacatgg
     9301 attgggttca gaaataggtc atcaaattat aggagaaaac tattttaatt gtaattgtgg
     9361 aaataatggt tgtgtagaaa ctttttgttc agcaactgca attataaagt attctcaaaa
     9421 actgatagaa gaaggagaaa agagtaggat tttagatttg gcagagggaa atttagaaaa
     9481 tgtaaatgca aagatggtgt ttgatgcata tagagaaaat gatttagtgg caattaaagt
     9541 aataaataga tttaaagagt atttagcaaa aacttttgca aacacaataa attctttaga
     9601 cccagaaatt atatctatag gaggaggcat ttctaaatct agcgatataa tacttgatgg
     9661 aatagaagat ttggtaagaa aatttgtatt atacaaaaca gaagatatag ctacaattac
     9721 atgtgcaact ttaggttcag acgcaggaat tataggagca gcttttttat aatgtaaatg
     9781 aactgcttat tggaggcgtg atgatatatg ggtaaaacaa atgctagaag aacgcttttt
     9841 ttgatagcaa taggttcagg aactatgctg aatcctctta attcttctat gatttcactt
     9901 gctttacata gtatacaaaa tgattttcat attagtttta caactgtatc ttggattgtt
     9961 tcagcatttt acctagctag tgctattgca caaccagttt caggtaagat aggagattta
    10021 ataggtagaa aggtcttatt tttgagtggt ttgatgcttg taattttatc agcatccata
    10081 gtaccacttg ttcaatcatt ctttatattg atttttataa gagtaattca agcaattgga
    10141 acaagtaccc tatatccatc tggagttgct cttatacaaa ataatataaa agaaagacaa
    10201 tcttctgcat tagcagttct aaccattttt gcttcaacta cagcaggatt aggtccgaca
    10261 cttggaggat tattgttgga cttaggagga tggcatgcta tatttcttgt aaatatacct
    10321 gttgttttgg tttctatatg cttagggtat tttttattcc caaaagaagt aaaagaaaag
    10381 aaatctataa aagaaacttt aaaaaatgtt ttgtcaagat tagatattat tggcatactt
    10441 ctattttcaa ttgcaatgat ttttatatta ctatttttgc tatctatgaa ggagtctttt
    10501 aacttagaac aacttgtatt tggtataata ctgatgtgtc tttttatttg gcatgaattg
    10561 agaacaaaat tgccatttat agatattaag ctttttgtaa gtaatcctaa attgtcaaaa
    10621 gtttacttgc aatttattat attaaatttc tttaattata ttttgttttt tggtcttcct
    10681 agttattttc aagatgcctt acattatagt gcaaagtcga ctggattatt tatgctattt
    10741 atgtctggta taggtatatt tatatctcca ttaactggaa aatggataga taaatctgga
    10801 actagatttc ctcttgttac atcatcaatc tttatgttta ttagtgcagt acttctttca
    10861 gtagttttta tccatatacc tgtaatagga aagggtatta tattatcatt agcaggtatt
    10921 agttatggag ttggtaatgt agccctgcaa tcctctatgt ttgaagaaag tcctcgtgac
    10981 agtataggga cagcttctgg tctgtttcaa actagtcgtt atttaggttc tattttagca
    11041 actgttttac ttggaatggt atttgaagta aatataacat cagaacaatt tcagatatta
    11101 ggttatgtta tggtggtctt aggtacaata agttttttat taaattttat attaaagaaa
    11161 gaattaagaa aagtagaata gtggatttat ggatttaata agtctaataa ctgataagtt
    11221 taaaaatatt gtttgtaaaa cataactggt ttgatattta ttaattctaa gttttgaagc
    11281 atagagtact cgataagtat acaatatttt gaattaaaat taagcagaaa tttgaactag
    11341 tctttcaaat ctctgcttaa tttagtagtt aaattttttt atagagaaaa taagatttga
    11401 cgcttagata aaaaatcatt gcattttcaa aattctttaa atcaaaccca agtatctcat
    11461 taatcttttc caatctatat actagcgtgt ttctatgtaa aaacatacta gtagatgtct
    11521 tagaaatatt taaatcatta tttataaatt ttattcctgt atatagaagt tcttcgtcaa
    11581 tagatttaat cttattaaaa tctattaatt tattgagtat attatcctca ttaattaaat
    11641 ctaaagtttt atatctaata aggtcaaaca aatttaaaaa cttattttca gcgttgtctt
    11701 ttataatatg aggtagtaaa tcagaacata actgtaatct aaaatcaaaa tcagctttac
    11761 tatctaattc accaagataa aaaatataag tactttgata agtttcagtt tcaattatat
    11821 tttgtgtagt tacattaata tctaagttat atttagaatt tactataaaa aaaccattgt
    11881 gttgtattat atttatatct tcaaataaat tataaaaaat attttttaga tattcaaagt
    11941 ctatatgaga ttggtaaatt aaataaaagt tgccttttaa attatcgaga ttatttttat
    12001 ctagaaaatc aataacataa atagcattat tgaaagtaac taaatggcta ctagcatcta
    12061 acttttggtt ttcatatgga tagatattta tttttttatt aagctgtctt tctaaaaaat
    12121 cttttaactc ttccataaag tctccttaaa aataagatta tattcttatc ttatagctaa
    12181 agtagtttct ttgtcaaata tgtgtactct actattgtca actgaaagtg ttaagtcatc
    12241 accaacatta attctgtttt tactgtcaaa tctaattgtt atcgtatttt cgcctagttt
    12301 taagtgagca taagtttctg ctcccataag ttcttttatt tcaattcttg atttaaaaca
    12361 agtatctgga ttagatttaa taaaagtatc ttctacatga atatcttctg gtctgatacc
    12421 tataacaact tcattattaa tataattgtc ttttattaaa tcatagtgtt tttctttatt
    12481 caatttaagc ttaatatgtt cattttgtgc ataaatttca tcattttctt ctattaatat
    12541 tacatcaata aaattcatct gtggtgctcc aataaatcct gcaacaaata tattagctgg
    12601 ataatcgtat acatcttgtg gagttgcaat ctgctgaact actccatcct tcattactac
    12661 gattctatca gccatagtca tagcttcaac ttggtcgtgt gttacataga taaatgtagt
    12721 tccaagctgt tggtgtagct tagttatctc tgttctcata gcagttctaa gttttgcatc
    12781 taagttggat agaggttcat ccattaagaa taccttagga tttctaacta ttgcacgtcc
    12841 taaggcaacc ctttgtcttt gtcctccaga taatgtttta ggttttttat ttaaaagagg
    12901 tagtaagtct aatattttag cagcttcttt aacttttttg tctatttcat ctttaggcaa
    12961 ttttctaagt ttaagtgcaa atgccatatt ttcgtaaaca gataagtggg gatataaagc
    13021 atagttttga aaaaccatag ctatatctct atcttttggt tctatattat tgacaagttt
    13081 atctccaatg tatagttctc cttcagagat ttcttcaaga ccagcaatca ttctaagtgt
    13141 agtcgattta ccacaaccag atggtcctac taaaacgata aattctttat ctttaatgtc
    13201 tatatttata ttttttactg cgttaaatcc atttgagtaa agttttgata tattttttaa
    13261 aattacttct gacataatat cctccttgat atttaaattt taattatata gtatcaaatt
    13321 tttttgttat attaaatgtg taaaatgact aaatgtattt gtttaatatg tatatttagt
    13381 ttttagcaaa gaataaaact aacagtaaag tatatttaaa aaattaaaaa acaaaattaa
    13441 atttattat
//
LOCUS       DACXMZ010000094        13338 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.095, whole genome shotgun sequence.
ACCESSION   DACXMZ010000094 DACXMZ010000000
VERSION     DACXMZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13338
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.095"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            218..1636
                     /locus_tag="JD016_10715"
     CDS             218..1636
                     /locus_tag="JD016_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAU5000108.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            1712..2524
                     /locus_tag="JD016_10720"
     CDS             1712..2524
                     /locus_tag="JD016_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAU5000109.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            2947..3564
                     /locus_tag="JD016_10725"
     CDS             2947..3564
                     /locus_tag="JD016_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAU5000110.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(3721..4173)
                     /locus_tag="JD016_10730"
     CDS             complement(3721..4173)
                     /locus_tag="JD016_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000111.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            4621..5979
                     /locus_tag="JD016_10735"
     CDS             4621..5979
                     /locus_tag="JD016_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAU5000112.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            6252..6632
                     /locus_tag="JD016_10740"
     CDS             6252..6632
                     /locus_tag="JD016_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAU5000113.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMSYGRMFSFKDQDGNDYLVREDK"
     gene            6757..6957
                     /locus_tag="JD016_10745"
     CDS             6757..6957
                     /locus_tag="JD016_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000114.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            7153..7881
                     /locus_tag="JD016_10750"
     CDS             7153..7881
                     /locus_tag="JD016_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAU5000115.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            7903..8820
                     /locus_tag="JD016_10755"
     CDS             7903..8820
                     /locus_tag="JD016_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HAU5000116.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            8962..10146
                     /locus_tag="JD016_10760"
     CDS             8962..10146
                     /locus_tag="JD016_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAU5000117.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            10265..10453
                     /locus_tag="JD016_10765"
     CDS             10265..10453
                     /locus_tag="JD016_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAU5000118.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            10602..11252
                     /locus_tag="JD016_10770"
     CDS             10602..11252
                     /locus_tag="JD016_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAU5000119.1"
                     /translation="MKKNYEIILFDLDGTLTDPKEGITKSIQYSLNSFGIEEDLDNLE
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            11355..12293
                     /locus_tag="JD016_10775"
     CDS             11355..12293
                     /locus_tag="JD016_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAU5000120.1"
                     /translation="MKSILIMGGSDFIGFALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            12319..13170
                     /locus_tag="JD016_10780"
     CDS             12319..13170
                     /locus_tag="JD016_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAU5000121.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         5331 a         1284 c         2348 g         4375 t
ORIGIN      
        1 ttaataaaaa aaaattcaaa ttataaatat taaatgaata taattaaaac aaatatataa
       61 atttaagtat aacactcaca ttttaatgta ttgtgaaatt tttgttgaac tttatgtacc
      121 ataacttata aaatttatgt catgttatat ttgaataagt aataaaattg tagatgtaaa
      181 ttaatctaca aaatttatat gaaatggagt tgatgctatg acagaattaa tgattataag
      241 tgggttagta ctattgattt gtataacttc tagcaaggta ttgtataaat ttggagtacc
      301 aatattattg atttttatct tactagggat gttatttggg tctgatggga tggtaggaat
      361 ctattttgat aactatgaac tgacaaaaca attatgttct attggactaa tatttataat
      421 gttttttgga gggtttggaa caaactggaa tatggctaaa cctgttgcta taccttctat
      481 attaatgtct tcactgggtg taataattac ggctggcgtt acaggtatgt tttgccacat
      541 ggtaatagga actagtttac ttgaaggttt attaattggg gcaattgcag cttcaacaga
      601 tgctgcatct gtgtttgctg tgttgcgttc tcaaaaacta aacttaaagg gttcacttgc
      661 atcattatta gaagttgaaa gtggaagtaa tgacccaata gcgtatatgt tgacactaat
      721 tcttttgaca ttgatgaata attctggggt agaacttatt gcaccaatgg taataaaaca
      781 aatagctttt gggttagcta taggatttat actagcaaaa ttatctgttt atattttaag
      841 acattcaaac ttcgagatag aaggatttta tactatattt ataacagcta ttgcaatact
      901 ttcttatgca tttagcgagt atcttggagg aaatggatat ttaagtgttt acatttcagg
      961 tattattata ggaaatagta ggatacctct taaaaaaagt ttggttcatt tctttgatgg
     1021 aatttcatgg attatgcaaa taatgttatt ctttatgtta ggattgttat ctttcccttc
     1081 taagttacca aatgtatttg gtatagcaat ggccatatct atattcatga tagttattgc
     1141 aagaccatta gcaacattta tagttctagc aaaatttaaa ttctcaaata aagaaaaatt
     1201 attcatatcg tgggtaggtc ttagaggtgc ggcttcagta gtttttgcaa tttatgctgt
     1261 tactcaagga gtagccatag agaatgacat attccatata atatttttta tagcgttgct
     1321 ttcagtagga atacaaggtt cattgatacc aactgtagcc agaaagttgg atttagttga
     1381 tgataataca cctgtattaa aaacatttaa tgattatgat ggagaaatta gctctaaact
     1441 tatcgaagta aatgttgatg agggtaataa atgggctaat aagagtataa tagattctaa
     1501 tataccagac gatatattaa tagttatgat aaaaagaaag gaacaggttt taatacctaa
     1561 aggttcaacc attataaaaa caggggatat tttggtattg agtggagata gaattgaaga
     1621 attaatacaa ttataattta ttttgtattg actttaacct aaggggaaag tttaaactta
     1681 aattaatcaa atctaatgcg caggtgatga gatgttttct ataggaatgt tttctaaaat
     1741 aaataaaata actacaaaga cattaaggta ttatgaagat atagggttgt taaaaccaga
     1801 atatgtagat gaatttacaa aatataggta ttatacaaca gagcaattac ctaaattaca
     1861 tcagataata actttaaaac aaatgggttt atcattagct gagataaaaa aagctgtaga
     1921 aaatcctgaa gaagttgaaa atattttaat gaataaagaa aaagaaatgt tggatgtaat
     1981 taaaagtgaa gaatataaaa taattaaact acgtaattac cttatgaatt tgaaaggaga
     2041 agtcaatatg aatgatgtaa ttattaagtc tttaccaaaa gttaaagttg cttctatgag
     2101 aaaagtgtta aaggggtatg atgaactttt taatctattt ccaaatgtaa tggctgaaga
     2161 aatgatgaaa acagggtgta catgtgcagt tcctgattat tgttttaata tatatcatga
     2221 tggtgaatat agagagactg atatggatgt tgaaatgtgt caagctataa atgatttaaa
     2281 ggaagatacg gatatattaa aatttaaaga attagatgca gttgaaaatg cagtttgtat
     2341 attacacaat ggtccatatt caacattagg aaaaacttac gcagatgcat ttaaatggat
     2401 agaggacaat aaatataaag tggttggaaa tcctagagaa tcttatatag atggtatatg
     2461 gaataaagag agtgaagaag actggttgac agaagttcag ataccagtga aacgcatgtt
     2521 ataatatgtc tacaaagtat aaataagaat aaaaaaggtg gtgtctcaaa gtaaacattt
     2581 tagtttatga gacactcttt ttcatatgaa atcaagtata tttttataat tacgattatg
     2641 aaaaaaggct tatctatatt tttagacagc ctcttatttt tttattataa tagttttcta
     2701 aagatgctct atttataaat ttttattaaa gactttataa ataattgtat ttaatataca
     2761 aaatatatat aaatattgat gttttatata taaagttgta tattttttta aggatataaa
     2821 gtaaaaaaat atacaatatc aagaaaaaaa gatattgggt atttaaagta atactttatt
     2881 taattttaag taaatcggat atctacatat atagatacta atttcaaaga ataggagggt
     2941 gtttatatgg aaatattcgc aataggacat tcaaattatc catatgataa gcttattaat
     3001 atgataaaaa aatatgacat aaattgtgta gttgacatta gagaaactcc atattctaaa
     3061 tataatattc aatacaataa agaggcattt aacgaatctc ttagaaattc cggattttta
     3121 tatatataca tgggaaaaga atttggagct aaaagaacta ataaagatgt atatacgcaa
     3181 gaaggttatg cagattttga gaaagtagca aaagaagata tttttttaaa cggaatagaa
     3241 agattaaaaa agggttgtca aatgggttat aggatagttt tattaggagc tatgcaagaa
     3301 cctataagat gtcatagaag tatcttggtg ggaaaagtat taaataaaga aggttttgat
     3361 gtaaaatata taatgcatga aggaaatttg gcatatcagg aagatataga ggaatcatta
     3421 ttggataagt attttagtga tagaaagcaa ctgagtattg acaatttatt agggagtgca
     3481 ttaacgagag aagaaatgat acaagaaggt tataagctag ctaataaaga aataggatat
     3541 agaactgaga aactaggtaa ataaaaatat acaagtagat aaaatacagt acttatctta
     3601 agtaaaataa tattatgaat ttataatagc taggcatgat gtgccaatta aaaatattct
     3661 agttatttaa taatatcatt aaatttaggg tattttttaa tagcacatca tgttgcagtt
     3721 ttaaaaaata aaatctctaa tattgccata atcaactgtt ctattaagag taggatgact
     3781 agatataaat ctaactgtaa tttttgaatt taacaaacaa ggatttgaaa ttaaaaatac
     3841 tctatctcca ttgacaatag aagtaatagc tactggcaaa atatctacaa atgtatcata
     3901 tctgttttta gcaatttgat aaggataatt tcctacaaaa acaaatagat ttaaatcata
     3961 tatattatca gtaaaccaag cccattgacc ataaactttg tcgatattca ttgtgcctaa
     4021 accaagtgta tcaaagtatg ttgctatagt taaataaata ttttccatgt tttctaaata
     4081 tgaaacagta tatcttctga aacgtatggg tgctgtggaa gaaataggat aaaccatatt
     4141 tacatttaga ttatttgcat ttaaattaac caaagtatca catcctaaaa ttagtctata
     4201 aaatacaata tttaaatgga tgcgattttg ttacatctaa agtgtaatat tttatatatt
     4261 gaaaaagtgg taaaagtttg gatggttaaa taatattata aaaaatatat aaaatgtact
     4321 tattgttatt gctacacgat agtgatacaa tattaacgaa atatagctac ttttaataat
     4381 aacggaaaat atttatatta aattaataaa tattttaaat gtggtatcca taaaaaataa
     4441 taaacataag ataattataa agatatactg tgttcaaatg atattaaaca agatttatat
     4501 tatttgaaag cagtatatta taatgcgaac aatacaattc taaactttgc attgtcaatt
     4561 tacagttcga taaactaatt aaaaattaaa tttatataaa gagggaaaag aggaataggg
     4621 atgtttataa ggaaaaatgt tataaagtta gcaattccta taatgataga gcagacgttt
     4681 gtcatgcttt taggagtatt taatacaatg atggcaggtc atattgggga agaagctgta
     4741 tctgcagttg gaatggttga ttcaataaac aatatattta tatctgtatt tgctgcacta
     4801 tctgttggag ctactgttgt tgtagctcaa caaataggaa aaaaagatag tgaaaaggtg
     4861 aatgaaacta ttaagcaggc tctagtgtct ggagtaatat tatcctttac aataacaata
     4921 ttgatgtggg tttttagaac tggtgtaatt aatttatttt atggttccgc agaagaattg
     4981 gtaaaatcaa atgcaaaatt atacatagag tttacattat ttacgtatcc atttattgcg
     5041 acacaacaaa tagcaaatgg tattttaaga gggtgtggag atgtaaagaa acctatgtat
     5101 gtgactatgt ttatgaatat agtaaatgta acattgggat atatacttat atatggtatt
     5161 gatttaaatc ctctaggaat aaatttgcaa acaccatcct atggcataac aggtgcagct
     5221 atatctattg ctatagctag aataattgga tgcatagtaa ttataagagc tttatttaaa
     5281 ggaaacgaat ttatatctat gggtaaaata tttccattta agattgatgt tgaaactcaa
     5341 aagtctatat ttaatatagg aataccagct ggagttgaac aactactttt taatgcaggt
     5401 aaattgattg ttcaagtatt tatagttact atgggaacat cagcaattgc atccaatgca
     5461 ataggaatgt caattgcatc cataataaat gtaattggga atgctttagc tttgtctgct
     5521 acaaccttgg tgggacagta tataggacgt ggagatatta aaggagctaa gagtacttta
     5581 ttatatttaa ctaaatttgc aacaatttgt ttatttatag taggagctat atttatacca
     5641 attgcaccct ggatttcaag tatgtatact caaaatgcta gtgtaattga catctcaaca
     5701 caattaattc gaagtgattg tttagctatg gtaatatggc ctatatcctt tgtattatca
     5761 gcagggctaa agggagccgg tgatactaga tatacaatga tgactgcagt aataggtatg
     5821 tggattttta gaatttttac aggttacgta ttgggggttg tattagaatt tggagtgtta
     5881 ggaatttgga taggtatgta tactgattgg cttgttagag ggactttata ttgtttaaga
     5941 ttaagaggaa caaagtggct aaaacacaaa gttgcataga atatatgaaa tcacttttag
     6001 atggataact ctatttagaa gtgatttttt atgtagttat tatcataatt ataagtatga
     6061 tatataaggt gattaaatta cataaataaa ggctataata gtatggatat tataaattat
     6121 atattgagca gaattacata atttggcgat aaatattaga aatatatatc tatagaaaat
     6181 aatatatatg ggtatattat taaatataaa aaatattagg tatttattta ataataaagg
     6241 tgggtattgt tatgataaat aaaataggaa aaataactct ctatgtaaat aatcaagagg
     6301 atgcaaagtt attttggaca gaaaaattga actttgtagt aacttatgaa aaggaaatgg
     6361 gtccaaatat gaaatgggtg gaagtagcac caagtaaaaa tgaatttaca acatttgtgt
     6421 tgtatgataa aaatctaatg atgaagcaaa acaaagagat aaatgtatct catccatcaa
     6481 taattttaag tacaagagat attaatggaa cttatgaaca aatggcaaat aacgaggttg
     6541 aggttgggga attaatggag atgtcatatg gaaggatgtt ttcttttaaa gaccaagatg
     6601 gaaatgatta tttggtcaga gaggataaat aattttacaa agtattaaat ttactcaata
     6661 tctgatataa ttagagacaa taattattat aaactagtct aggattagaa ttatataatt
     6721 aaaaattaag tgtaaatttt gagtagaggt gataaaatgt ttaagaaaat ggcagtacta
     6781 aaagatatag caactaaaat aggtcgtaaa aaagcatatg aactattaga aatggttgaa
     6841 ggtaatgatg cctttgtagc tgaggtaaag ataaaaaaga atggaataga atctaaaaaa
     6901 gaagaaatta tgttaaaaga taatcaaaaa ataatattag agtatataga aggttaaatt
     6961 ttaaatacat ataagttgat atactaaatt tctgagatga gtttacataa aactcatctt
     7021 ttttactttt caataacttt gttttattat ttaatataca ttttatctcc ttagagttgc
     7081 tgatatataa aaattactga tataataaaa gaatggatat taatgtaata ttttatagaa
     7141 aagaggggta ttatgcttga aataaaaaat ctttccttta atgttgagtc taacaatgag
     7201 gaactaggaa taataaatga tgttagcctt tcatttgaga ggggaaaact tattgttatc
     7261 acaggtccaa atggtggagg aaaatctaca attgctaagc ttattatggg aattgaaaaa
     7321 gctacgagtg gtcagataat tttagatgga gaagatataa caaatcttag tattacagaa
     7381 agagcaaaaa aaggaatagg atacgcattt caacaaccac caagaataaa aggtatgaca
     7441 gttgaaaatt tacttacact tgctcatgga aaaccattgt ctactgatgt atgttgccaa
     7501 taccttacag atgttggatt gtgttcaaaa gactatttaa atcgtgaagt tgataactct
     7561 ctttctggag gggaaatgaa aagaattgag atagcaactc tttttgctag agatttaaag
     7621 gtttctatat ttgatgaacc tgaagcaggg atagaccttt ggagctttgg aaaactcaat
     7681 gaaagtttta agaaaataca tgaagagtct aatcagacta taatcataat ttctcaccaa
     7741 gaaagaatat tagagttagc tgatgaaata attgttctac aagatggttc agtaaaaagc
     7801 catggaacaa aagaggctat attacctgaa atcatgtgtc aagttaattc aagttgtgaa
     7861 ttaatgaaag acatgaatta attagaaagg aaggcaaatt aaatgaacaa aatagattta
     7921 aatcttttag aaaaagttgc tgaacttcgt ggaactccat taggagcata caatatacgt
     7981 aaaaatgggc aaggagtaga gagacattca actgaaaatg taactataat tcctaaaact
     8041 gataaacctg gaatagatat tatagtaaag ccaaatacaa aaggagaaaa tgtacatata
     8101 ccagtaatag taacagaggc tggtgtacag gatatggtat ataatgattt tgaaattgga
     8161 gaaaatgcag atgttgttat aattgctggg tgtggtatac ataactgtgg ttgtgatgat
     8221 agtgaacata atggtataca tagatttttc ttacgtaaag gagctcgcct aaaatatgta
     8281 gaaaaacatt atggtgaagg tgatggagaa ggaaagagaa ttttaaatcc agttactatt
     8341 gtcaatatgg atgaggattc atattgtgaa atggaaacaa ttcaaattaa gggtgtagat
     8401 tcaactaaaa gagaaatgga tgcaaaccta ggtaaaggag ctaaaatagt tgtatttgag
     8461 aaattattaa ctcatggtaa tcaagttgca gaatctaata tgaaggttat cttagagggt
     8521 gaaaactctt ctgctcaagt agtttctcgt acagttgggc aagataattc taagcaaaag
     8581 tttaatcctt gtgttattgg ggattccctt tgttctgcac atgttcaatg tgattcaata
     8641 attatgggaa attctcaaat tagtgctata cctgaaattg ctgcaaatca tccagatgct
     8701 cttttagtac atgaagcagc cattggaaag attgcaggag accaaatact aaagatgatg
     8761 acattaggtc ttactgaaga agaagccgag caacaaatac ttaattgctt cttaaagtaa
     8821 attaaagatt ttatttaatt taatataatt aatatagtat aatatagtga ataagtctaa
     8881 aatattttta tttttaggct tattttcttt atgtaagaaa agctattaga gaaattatat
     8941 tgtaattagg atgtggaata aatgaaaaat ttacttataa gaaatgggac aataatatct
     9001 ccaacagatg gtatcaaatt tgaaaaaaga gatatcttgg ttttacatgg gaaaatatat
     9061 gctattggat tgaaccttaa agagaaaata agaaaagaaa aattacttac aaataaagag
     9121 ttcgagataa taaatgccga taatatgtat atttccccag gatttataga tgttcacacc
     9181 cattgttata agagaaaatt acctacaggt atggattctg attcaattgg aattgaaaaa
     9241 ggttctacag ttatatttga tgcaggtaca gctggtcctt caaactacta tgattttaaa
     9301 aaaaagtata tggatgagtg taaaacagaa gtatattctt ttttaaatgt tacaaatgag
     9361 ggactaaata tattgaatga atcaacttct ttagatgtta taaattttga caaggttgag
     9421 tcaattgtag aaaaaaatat agaaaagata aagggtataa aagtaaaagc gagtaaattt
     9481 caatctaatg aaagtggtat agatataatt aagaaatcaa agcaagttgc taaaaaatta
     9541 aatttaccct tagtggtata tataggagag tatccatcat atatagatga agtaatgaat
     9601 attttgggaa aaggagatgt ggttacaccg gtatatggaa atagtacaaa tggaatcctt
     9661 aaagaatctg gcacattaag aaaatcagta ataaatgcaa agaatagagg agtattgttt
     9721 gatgtatgtc atggagtgga ccaatttgat tttaaagttt ttaaaaaagc aataaagcaa
     9781 ggtttagagc ctgacctaat aagtactgat atgcatataa aaaacatgaa agacgtaaac
     9841 tattctcttt taaatgttat aaataaaatt atggaacttg ggatatccct agaaaagtgt
     9901 atagaaaaag taactgaata cccaacaaag attttcgatt tacatggaaa taaaggcaga
     9961 ataaagattg gtggagaagg tgattttaca atattcacaa tggaggagtg taatgatatt
    10021 ataaaagact acaataataa tgaacttgtt ttaaataaaa aattaagatt gcaatatact
    10081 gtaaaatcat ggatgaaaag cagtgaagtt tatagacatg gatacgaaaa tactataata
    10141 aactaaacaa tagtgataaa ataataaata tatgtttaaa ttggtatata ttggataaat
    10201 atctaaggaa acaacaattg aaacaacaat taaaaaataa taattttagg ggaggtattt
    10261 tagtatgaaa gcatttgttg ataaggatgt atgtgtagct tgtggagcat gtataggaac
    10321 atgtccagac gtttttagta tgagcgatga agggcattca gtagcaattg aaggagaaat
    10381 accagcagat ttacaagatt tatgtaaaga agccgaagaa ggatgtccag tttcagctat
    10441 aacagttaaa tagagctgtt taaatttatt tttaaaataa atatataaga tttaattggt
    10501 acttaagcta tagtattaga aacttctatt actatagctt tttaaaatag ataaacttaa
    10561 tatataagat attacttttt ataaatatgg aggactgaaa tatgaagaaa aattatgaaa
    10621 taatattgtt tgatttagat ggaacactta cagaccctaa agaaggaata acaaagtcta
    10681 tccagtattc tttaaatagc tttggcatag aagaagattt ggacaactta gaacaattta
    10741 taggaccgcc attacatgat acttttaagg aatattataa atttgaggat aaaaaagcta
    10801 aagaagctgt tgaaaagtat agagaatatt ttgctgataa agggatattt gaaaataaaa
    10861 tttatgagaa tatgaaagag atattagaaa tgctctataa aaatggtaaa atattattag
    10921 tagcaacttc taagccaaca gtttttgcag aaactatact tagatatttt gatatagata
    10981 gatattttaa atatatagca ggtagcaatc ttgatggtac aagagtgaat aaaaatgaag
    11041 ttatacaata tgtactagac ctatgtaatg taaaagataa agataaagtt attatggttg
    11101 gagatagaaa gtatgacata attggagcta aaaagatagg tatagattct attggagtat
    11161 tgtatgggta tggttctttt gaagagctta gtgaaagtga acctacttat attgtcgaaa
    11221 atgtagagtc tataaaagat attttgttat aaatatcttt tatagacaaa aatattacaa
    11281 aatggtgtcg catatttaaa aatatgatat tatagttaag gaagtcattt aaaaaagagg
    11341 aggatgcact taaaatgaaa tctattttaa ttatgggtgg aagcgacttc ataggttttg
    11401 cacttgctaa gcgcttgatt aaatgtgggt atcaaattga tatacttaca aatggtaaaa
    11461 aggaaataga ttataatggt tttaaagaac atctaatttg tgatagaaaa gttcgaaaag
    11521 atatggaaaa cattatcaca ggaagaaaat atgattacat atatgatatg acagcatata
    11581 caaaagaaga tgtaagtaat ctgattgatt ttataagcat ggataactta aaaaagtata
    11641 tagtattgtc agcaggagca gtatataaag atagtggaag aaatataaaa gaagaaaatg
    11701 agaaaggtga aaatgaaaac tggggaaaat atgggttaaa taaaaaagag gctgaagatt
    11761 ttattataaa tagccctata ccatatataa ttattagacc aacttacata tatggtgaaa
    11821 ataataattt atatagagag tattatttct ttgaaaaaat agaaaaaaat gaaaagatac
    11881 cagtaccaaa aggaaaacaa gttagtaatc aatttatata tattggggat ttggtaaagg
    11941 tattggaaag cataatgaaa aatcctcatg taagagaggc ctataatgtg acaaatccac
    12001 agttaatttc ttgggatgac cttatatata catgtggaga aattattggc aaagaaccta
    12061 taataaaata tgtagatatg gaaaaagtag agtttagaga aagaacgtat ttcccattta
    12121 gaaatataga ctttaatctt gatataaata agcttattga acatgggctt tatataccaa
    12181 acgtgttact aaaagaaggt cttacagcaa catataaatg gttttcagca aataagccaa
    12241 aaatgcatga cagaaaaatg aacaaagttg agtctgtaat gcaaatagtt taaaaaaata
    12301 aataagtagg tagatatttt gaactcaaaa attaagtatt ttttaaaatt ttttatctta
    12361 atatttattt tgatttttat atactcagtt gcgatagagc cgagtctatt aattgtgaaa
    12421 aaatataata taagtgaaaa tggagcattg ataaaaaatg atacaattga gagtaaatct
    12481 gaaagtgtaa aagtagttca aatttcagat actcaaatag gtagttttta ttcaactaag
    12541 aatctcaaaa aagtagctaa taagattaac actttgaatc cagatataat agtatttact
    12601 ggtgatttaa ttgattactc caataaaaat ccaagtgtag atgaaattac aactatttta
    12661 tcaagtatga atgctaggtt gggaaaattt tcagtatttg gcaatcatga ttatatgtat
    12721 aaacttccta ggtattatag gcagattatg aaaaattcca acttcaatct tttagtaaat
    12781 gaaaataaaa aaataaaatt aaaagatgat aaatatatta atatattagg tgttgatgaa
    12841 atattaaatg gaaatcctaa tataaaatat ctggagagtc aaatagataa taaaagtttt
    12901 aatttattgt tagcacatga accagattta gtagatatgc ttagtaaaga tacaatgaat
    12961 ttagttctat ctgggcatag tcatggtggt caaataagat tacctattaa gggagcatta
    13021 gttactcctc catatggaag gaaatatact aaagggtttt atgatataaa tggaaatcat
    13081 ttatatgtga gttcgggtct tggaagtaca aagttaccat ttagattttt taatatacca
    13141 gagataatag aatttaatat tataatctaa cataaaatat ttagaaaata taattttgct
    13201 ttttataata tgtagatgta tatactgtaa aaatggatta aaccagtaga ttgaatgaat
    13261 taatagatta aattaatata taaaatattt aagttataat taatgtatta gacaaaataa
    13321 atactattaa gaactatt
//
LOCUS       DACXMZ010000095        13299 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.096, whole genome shotgun sequence.
ACCESSION   DACXMZ010000095 DACXMZ010000000
VERSION     DACXMZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.096"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..318)
                     /locus_tag="JD016_10785"
     CDS             complement(<1..318)
                     /locus_tag="JD016_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAU5000122.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(438..1175)
                     /locus_tag="JD016_10790"
     CDS             complement(438..1175)
                     /locus_tag="JD016_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAU5000123.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1390..3213
                     /locus_tag="JD016_10795"
     CDS             1390..3213
                     /locus_tag="JD016_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAU5000124.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3428..7726
                     /locus_tag="JD016_10800"
     CDS             3428..7726
                     /locus_tag="JD016_10800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAU5000125.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            7930..8391
                     /locus_tag="JD016_10805"
     CDS             7930..8391
                     /locus_tag="JD016_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAU5000126.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8421..9575
                     /gene="nusA"
                     /locus_tag="JD016_10810"
     CDS             8421..9575
                     /gene="nusA"
                     /locus_tag="JD016_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAU5000127.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            9582..9863
                     /locus_tag="JD016_10815"
     CDS             9582..9863
                     /locus_tag="JD016_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAU5000128.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            9853..10164
                     /locus_tag="JD016_10820"
     CDS             9853..10164
                     /locus_tag="JD016_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7ae"
                     /protein_id="HAU5000129.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10186..12126
                     /gene="infB"
                     /locus_tag="JD016_10825"
     CDS             10186..12126
                     /gene="infB"
                     /locus_tag="JD016_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAU5000130.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12169..12549
                     /gene="rbfA"
                     /locus_tag="JD016_10830"
     CDS             12169..12549
                     /gene="rbfA"
                     /locus_tag="JD016_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAU5000131.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            12530..13207
                     /locus_tag="JD016_10835"
                     /pseudo
     CDS             12530..13207
                     /locus_tag="JD016_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
BASE COUNT         5286 a         1536 c         2513 g         3964 t
ORIGIN      
        1 cttatccata ttttttatgt aacctctttt ttctagcttt tttatatgct ccttacgtat
       61 ttcatcaaga ttaataccat attgttcttc tagcttaaac atcatagtaa cacatgtctg
      121 cgctacatcc attagttcct tagcagtttt ctttataata tcaacttcat ctaaatcatt
      181 attttctcca cttagatttc tgaatttacc tatacattca gctaattcac cttgttcttc
      241 acccattttg ataaatgtag attctagtgt tgggtctaaa ttattaagct ctggtaaaga
      301 tatagttttt agtttcataa aagctccttt caagttattc gtatttatac taaaacatca
      361 taacaaaaat aatactaaaa aaaaacttta aattcaacat aaaaacttac atatataata
      421 aaaatcgtga aataaatcta tatttgtctt aatacagcct catataaaac ttctccaata
      481 ggtttattta ttacaagaga agctgactta tcatagactg tatttgcttt attaataaga
      541 gcaatataat cacctttgta ataatctata aaacttgctg ctgggtacac agctaatgat
      601 gtacctccta taatcaataa atcagcatta cttatagcag aaattgtttt agtaatgaca
      661 tcactgtcaa gagcttcctc ataaagtact acatctggtt ttactataga accacagttg
      721 tcacagtaag gaatattacc tcccagattt aacatagatt ctaagtcaaa aaacttacca
      781 catttagtac aataatttct atgtacagaa ccatgaagtt ctagcacatt tttacttcca
      841 gccatttgat gaagtccatc aatattttga gttatgacag ctttaagctt acccatttct
      901 tcgagttttg ctaaagctat atgagcatta ttgggttttg cattaggata tattaattta
      961 tccttataaa attcaaaaaa ttcttctgga tatctaacaa aaaaagtatg tgaaactaat
     1021 tgttcagcag taaattgttt attgagtttt tggctaaata atccagtaga acttctaaag
     1081 tctggaatat tagattcagt tgaaacacct gctccaccaa aaaatacaat attattgtga
     1141 ttagctatta agtcttttaa gctatttgca tccatagtta cacctccaaa ttattatata
     1201 taaattgtac tacatattag gtcgtttatg ttttattaat ttaaataaat atatagttta
     1261 ataagttaaa taaagggaat ataaataaaa tgtatacaga gtataaatag acaatattta
     1321 ctaaaataac aagttgtcgt ataattatat taaaattgtt ttagtgtata agaagggcgg
     1381 gggaagtacg tgaagaaaaa agcagcttta gcaacattgg ctatgttacc cttaggtgtg
     1441 gtaaatgcac atgctgatgg agatataggt atagtgacta taaattattt aaatgtaaga
     1501 aatgaaccaa ctgccgaaag tagcatagcc tttgttgcta aaaaagatga taaagttttg
     1561 attaaagact cttctaatgg atggtataaa ataaaagctg aatctggaca agaaggttgg
     1621 gcttcatcaa aatatatagc aaaatcaaat agtgactctt taagaacgtc tacaaataaa
     1681 gaaaaacaag ttatttcaaa tagtttaaat atgagaaatg gagcaggtac aagttacaga
     1741 gtaataactg tactaaaaaa aggtcaaaaa gtagaagtaa tatcagagag taatggatgg
     1801 tctaaaatca aatatgatgg aagactagga tatgtatcta gctcttattt aggagacgtt
     1861 tcaaactcaa ctaataaatc aaaaactaaa caagtgaata caacttcact aaatgtaagg
     1921 agtggaccaa atacaagtta tggtttgtta ggcaagttgc caaaaggaag taaagtagaa
     1981 gtaatatcag aaagtaatgg atggtccaaa ataaagtata atggaaaaga tgcctatgta
     2041 tctagtatgt acttatcgga tgtaagtcaa agtaattcag acaattctag tcaaagcaat
     2101 gataaaaaga atactgataa ggtcgtaaat acagcttctt taaatgtaag aagtggacca
     2161 ggctctacat atagtaagtt gggaaaagtt tataaaggaa gtaaagtaac tgtactatca
     2221 gaaagtagtg gatgggctaa gattaatttt aacaataaag aagcatttgt agtaggcaat
     2281 tatttatcta cttcagcaga tacttcaaat aataactcaa atagtaactc tgataacagt
     2341 tctaatagta atggcaataa ttcttcatca tcaggtcaag taaatggtat gtcaggtata
     2401 agtggggcta aaattgatta taaatctttg agttatactt tagaatctca tataagtaaa
     2461 caagttgaga aagcagcatc aggaggaaat gtgatagctc caagtaatag gaaaagcact
     2521 ccaagtcctg aatttagtac tttttcagct caaagaacaa gctcatttgt aaatgcaagt
     2581 tcaagtgata tagaatatta tttaaatcct aaaaatttta caaatactac taaaggtatg
     2641 atgcagtttt taaagattaa cagttataga gatggtattt cagaatcaag tcttaactcg
     2701 tatcttaatg gtttatcttc tagtgtattt aaaaaccagg gagcagcctt tataaatgct
     2761 gccaagaagt ataatataga tgttgtatat ctagtatctc atgctatgtg ggaaactgct
     2821 tatggtaagt ctacacttgc ccaaggtcaa acattaacat cttataaggg acaacctctt
     2881 agtaagccag ttaaagtata caattttttt ggtataggag caatagataa aagtgctaat
     2941 gtttcaggtg cagaagcagc gtattccaat ggctggacta gtgtagaagc tacaatagat
     3001 ggctctgcaa agtggatatc acaaaattac gtaaatagtt ctaagtacaa tcagaatact
     3061 atctacaaga tgaagtggaa ttatgattat acatggcatc agtatgcaac tgatgtgaac
     3121 tgggctaatg gaatttctgg aattatggaa aatttaattg gtctttatgg aggaggaagt
     3181 agtttggttt ttgaagttcc tcaatacaag taatacataa ataaaaaata tagactatgg
     3241 gtagttttca ttttatttaa attatctata gtctttttgt atataattta taatctgtaa
     3301 atttaatgct aacaaaactt ttatgttata atattaattc taagtaaata taagtgagta
     3361 ataggtagaa atagataaaa agtggtaata atatatataa agataatttt gagaaaggag
     3421 gatatatatg gaaagtatta aggaatattt ggataagtta gaaattaata atagtggtct
     3481 tggaaagcaa cttaaagaag tttatataaa tagagtagtt tattttaagg aagataagat
     3541 agtttatttt tatctaactt ctaaagatat tgtttctcat gaacttttag ataaatttaa
     3601 agaagaattg atgtataaac tcgattattt taaagatatg aggatgaaga taagatttac
     3661 tggtcttgaa agaaaatcca ataaagatgt tataaaaaaa tattggaata atatattgta
     3721 tatattaaag tatctttgtc catctattgc agggtggtat aaacaagtag aatttctttg
     3781 tttagaagaa gaattaaaaa taaaacttcc aaaaggtata ttttatgagc gattaatgaa
     3841 aaaaaatgta acttatgtac ttaaaaccgt gcttagcgaa gagttgggat tagatttaaa
     3901 tataacaata gaaaaagctg ttgatgaaaa ggtaaataaa gaaagactca taagaatcaa
     3961 tgatagagaa atggaagaaa aaattagggc tttagaaatt ggaaaagtta ataactgtga
     4021 agaaaatgaa gaggaaagtt atgtcattaa gtctgatgta gatgaaaatt taatctatgg
     4081 agataatgca aatgctatga ttgaaaatat agttgaatta aatgcatctt ctggaacagt
     4141 tgcagttgtt ggagatatat ttgatgttga cactaaagaa cttaaaaacg gaaaaatact
     4201 tatgatagct tcaattacag attatactag ctcaataagc tgtaaacttt ttttaacaga
     4261 tacaaataag gatggagtac ttgaaaatgt taaaaaagga gcatacttaa aaatcaaggg
     4321 tgacatagtt tatgatacat atcaaagaga aattagtatg atgattagtg ggataagaaa
     4381 agaaactaga atagagagag aagataaatc agaggaaaaa agagtagaac tccatgcaca
     4441 tacacaaatg tcttctatgg atgctatttg ttctgttaaa aagcttgtag agcgtgcagc
     4501 caaatggggt cattctgcta ttgctataac agaccatggt gttgtacaag gatttccaga
     4561 tgctatgaat gcaggtaagt caaataatat aaaaatactt tatggagtag aagggtactt
     4621 ggttgaagat gattctctca ttatagagga tgcaaatgat aaagaacttt cgcagacatt
     4681 tgttgtattt gacatagaga caactggttt ttctaataca aatgataaaa taactgagat
     4741 tggtgctgta aaaatagaaa actttaagat tgtagataga tttagtgagt taataaatcc
     4801 tgaaaaagat atttcttata agatacagga attgacagga ataactaatg aattaattaa
     4861 agacaagcca accattgaag aagttcttcc taagtttatg gaatttgttg gagatagtgt
     4921 attagtagca cataatgcag agtttgatac tggatttata tcgcaaaaat gtagggaaca
     4981 aggcttgtta tataataaca aaaaagtaga tacacttatg ttagcaagag taatgttgcc
     5041 aaacttaaaa agacatagat taaatgtcgt tgcaaaagaa cttggtattc ctcttttaaa
     5101 tcaccataga gcagtggatg atgctgaagc tacagcactt atatttaata agtttttgca
     5161 gatgcttaca gaaaaaggtg caaaaactct aagtgatgta aacaatatac ttggaaagat
     5221 tgattacaca aagcttagca caaatcatat aaccttgata gcaaaaaatt ctataggtat
     5281 aaaaaattta tataaaataa tttcagatgc acatgtaaat cattttttta gagcaccaag
     5341 aatccttaga agtgtattaa atgaatataa agaaggatta ataattggtt cggcttgtga
     5401 agctggggtg gtttttcaag ctgtaaagaa aaacgtgtct gatgaggaaa tgaaaaaaat
     5461 aatagattta tacgattata ttgaagtaat gcctatagat aataatagat ttatgattga
     5521 taaaggagaa gtaaaggacg aagaagagtt aagggagttg aataggaaac taattgatac
     5581 agccaaaaaa tttgacaaaa ttccagtagc tactggggat gttcattttt tagacaaaca
     5641 tgaagctgtc ttaagaaaag ttcttaaata ttctcaaggt tttaaagtag atgaagaaga
     5701 aacttattta cattttagaa caacagatga aatgttggaa gagtttagtt atcttggaga
     5761 agaattggca tatgaagttg tagttgaaaa tagtaatctg attgccgata tggttgaaga
     5821 tataaaacct ataccagatg atacttatcc tccaattata gagggctcag atgtggagtt
     5881 aagagaaatg tgttacaaaa aagccaaacg aatctatgga gacccaatac cagaaatagt
     5941 tcaaaaaaga ctggatagag aattaaattc aataataagc aatgggtatg cagttatgta
     6001 tattatagct caaaaactgg ttgcaaaatc tctttcagat ggatatttag ttggttcaag
     6061 aggctcagtt ggctcatctt ttgcagctac tatgagtgat ataacagaag ttaatcctct
     6121 tccagcacat tatatttgtg aaaacgaaga ctgtaaatat tcattctttt atgaaatagg
     6181 tgaatggggc tcaggtgttg atttaccaga caaggattgt ccaaagtgtg gaagaaaact
     6241 tgctaaaaac ggtcatgata ttccatttga ggttttctta ggttttgaag gtgataagga
     6301 accagatata gacttaaatt tctcaggaaa ttatcagcca acaattcata aatacacaga
     6361 agaactattt ggagaggggt atgtatatag ggctgggact ataggtactg tagcagaaaa
     6421 aacagctttt ggatacgcaa gaaaatatgt ggaagaaaat aatataagtg taccaaatgc
     6481 agaagtactt aggctttcaa atggatgtac tggagtaaaa agaacctcag gacaacatcc
     6541 tggtggagtt atggttatac cagactataa agatgtatac gattttacac ctatacagta
     6601 tccagcaaat gatacttctt gtggtgttat aactactcac tttgattatc attcaataag
     6661 tggtagaata cttaaacttg atatacttgg acacgacggt cctaccatta taaggatgct
     6721 tgaagatatg actggaataa atataacaga aattccttta gatgataaag agactatgtc
     6781 tctatttaca tctacagaag cattaggtgt tacaccagaa gaaataaact gtcctatagg
     6841 ttgccttgct gtaccagagt ttggaactaa gtttgtacga caaatgttac ttgacacaaa
     6901 acctactaca tttgcagaac ttgtacgtat atctggtctc tctcatggga cagatgtttg
     6961 ggtaaataat gcacaagact tagttagaga agatatagtt ggattaaaag atgttatatc
     7021 tacacgagat gacataatga actatttaat atttaaagga ttacctccca aaatgagctt
     7081 tacaataatg gagagtgtaa gaaaaggtaa aggtttaaaa ccagaacaca ttgaagaaat
     7141 gaaaaaaaat aatgttccag aatggtatat aggctcatgt aaaaagatta agtacatgtt
     7201 cccaaaagca catgctgttg cttatgtaat gacatcattt agaattgcat attgtaaagt
     7261 tcactatcca gaagcatttt atgcgactta ttttacgaca aaagttgagg attttgatgc
     7321 agatttaatt gttaaaggat tagatgctat aaaatcaaaa ataaatgaaa ttgagtcact
     7381 gggaaatgat gcaaccacaa aagaaaaagg tatgttgacc gttttagaag ttgctcttga
     7441 aatgtatgct agaggaataa aattattacc agttgatata tataaatcag atgcaactga
     7501 gtttatagtt gtaggagaaa aaacattact tccaccaatg gcagcaatac aaggtcttgg
     7561 aggaaatgcg gctataaaca tccaaaatga aagaaaaaat ggtgaattta tatcaaaaga
     7621 agatttgaga aagagaacaa aaatatcaaa aacggtaata gaaactctta ctaatcatgg
     7681 ttcacttgag aatatgagtg agagaaatca attgtcattg ttttaagtta gcccaaaaag
     7741 ttgcaaataa aagaatttta tgatatcata gtatagaaaa tagtatactt tgcagaaact
     7801 aagagtgaga cagctgttct cactctttta ttttaaatat attattaaaa agctggttta
     7861 tttttattaa atatatttta tttaagatta tataaactgg tcatttacaa ttgaatagga
     7921 ggaactttaa tgaaaaaaaa tttagaagct acaattgagg agattgtaac taaaataacg
     7981 gatgaacatg gatttgaaat ggttgatgtt gaatatgtaa aagaagcagg agaatactac
     8041 ttaagagtat acatagataa agaagaagga atatctttaa atgaatgtga attagttagt
     8101 agagaattaa gtccaatact agatgaaaaa gaccctataa aagaaaatta ttttttagag
     8161 gtatcttctc cagggcttga tagagcatta aaaaaagaca gagattttgt tagatatcaa
     8221 ggtagagatg tagatttaaa attatataaa ccattaaatg gatgtaagca atttgaaggt
     8281 gaacttgtag gattaacaga agataataat ataaaaatca ttgcaaatgg taaggaaatg
     8341 gaatttaata gaaaagatgt tgctattgtc agattagcaa taaagtttta gtggtatatt
     8401 aaatagggag gatataaaaa atgaatcatg aatttatgga agcattagat gaattagtag
     8461 ttgacagagg tatagataaa gaaatactta tagatactat agaacaagct cttttaactg
     8521 catacaagaa aaactttggt tcagcacaaa atgttagagt tgaatttgat agagacaagg
     8581 gagatataaa agtattttct caaagagttg ttgttgatga atcagattta tatgatactt
     8641 tcttagaaat agagttagca gatgctagag agataaatcc taattatgaa ttaggtgata
     8701 ttatagaaca tgaagttaca cctaaaaact ttggaagaat tgcagctcaa acagcaaagc
     8761 aagtagttgt tcaaagaata agggaagctg agagagaaat agtgtataat gaatttatag
     8821 aaaaagaaaa tgaaatagtt acaggagagg ttgctagagc aaataaaaat gttgtttatg
     8881 taaatcttgg aagaatagaa ggtataatga ctcaaagtga acaaatacca tttgagaatt
     8941 ataaagcagg tcaaaaaata aaattctata tacttgaagt taaaaagact aacaaaggac
     9001 ctcaaatagt tgtatcaaga agtcatccgg gtctagtaaa gagattgttt gaagaagaag
     9061 ttcctgaaat atatgaagga atagttcaaa taaaatctgt agcaagagaa gcagggtcaa
     9121 gaactaaaat ggcagtaaga tctatagatg aaaaaataga cccaattgga gcttgtgttg
     9181 gaccaaaagg acttagagta aaaaatatag tggatgaact tggcgatgag aaaatagata
     9241 taataaaata tagtgaagac ccagcagagt ttatatcagc agcacttagc ccatcaaagg
     9301 ttgttagagt agatatagat gaagatgaaa agtctgcctt agtaatcgtt ccagattatc
     9361 agttatcgct tgctataggt aaagaaggtc aaaatgcaag actagcagca aaattgacta
     9421 actggaaaat agatattaag agcgaatctc aagcagaaca agaaatgtta aatcttgaaa
     9481 attccaaaga aatagaagct gaggatgttg aagctgaatt tatcgaccaa gaagagttag
     9541 atacttcaga ggataatttt agtgaagaag agtaaggagg gatgattttg aaaaaagtaa
     9601 aaaaagtgcc tcaaagaaaa tgcatagcat gtcaagatag agactctaaa aaagagctta
     9661 taagaatagt taaaaataaa gaaggaaaaa tatttttaga cccaacagga agagccaatg
     9721 gaagaggagc atatgtatgt aaaagctctg aatgtctaaa aaaagctata aagagtaaag
     9781 cattaaataa agcttttaaa atagatgttc ctgatgaagt ctatgataat ctacttatgg
     9841 agttagaaga atatgaaaaa taataaagaa aaagtacttt cgttcttagg gctcgcaact
     9901 cgtgcaggga aaatagtgtc tggtgatgat tcaactttgc ttgaccttaa aaaaggaaaa
     9961 gtaaatttga ttttaatcgc agaagatgca tcaaataata ctaaaaaact ttttaaagat
    10021 aaatcaactt ttagaaatat tccatatcta tttttttcaa caaaagagga aataggattt
    10081 gcaataggaa aatctccaag agcagttgta ggaataaaag atgaaaattt ttctaaaaag
    10141 ataattgaac taatagaaat ttaaaataaa atagggggtg atcttgtgtc aaaaacaaga
    10201 gtataccaaa tagcagaaga attaaatata tcaaatgaag agcttataaa taagttagct
    10261 gaattagata taaatgtaac tgacaaagat tcagtattag aaggtgaaga gttagaatta
    10321 gcacttgaaa tgttaggtga agatttatct caagaaaatg gaaatgttat agaaatagat
    10381 ggaaagttga cagtacaagt attagcaacg aaattagata aatctccatc agaaataatt
    10441 atgaagctta tgaaaatggg aactatggca acaataaatc aagagataag ttttgagatt
    10501 gctgctttgg ctgccaaaga ctatggattt gaattaacag tagctgaaag tgatgataca
    10561 gaagctttag aaatagaagc tttaatggaa attgaagaag acaaagaaga ggatttaaaa
    10621 cctagaccac cagttgttac agttatggga catgttgacc atggtaagac atctttacta
    10681 gatgcaataa gaaaaactga tgtgatctca ggtgaagctg gaggaataac tcagcatata
    10741 ggtgcttcag aagttaaaat caatggacac aaaatagtat ttttagatac tccaggacat
    10801 gaggctttca catcaatgag agcaagagga gcacaagtta cagatatagc aatattagta
    10861 gttgcagcag atgatggtat aatgcctcaa acagttgaag ctataaacca tgctaaagca
    10921 gcaggtgtac ctttaatagt tgctattaat aaaatagata aaccaggtgc aaacccagat
    10981 aaggttaaac aggaattagc agaccaagga ttattagttg aagactgggg tggagaagta
    11041 atagcagttc cagtatcagc taagaaaaaa gaaggaatag atacactttt agaaatggta
    11101 ttattggttg ctgaaatgga agagttaaga gctaatccaa ataaaagagc agtaggaact
    11161 gttatagaag ctgagcttga taaaggaaga gggccagttg caacagtact tgttcaaggt
    11221 ggtactttaa cagtaggtga cccaatcgtt gcaggtgttg catgtggtaa agttagagca
    11281 atgataaatg caaaaggtaa gagagtaaaa acagcaggac catctacagc agtcgagata
    11341 ttaggtctat cagaagttcc acaaggagga gaccaatttg tcgaagttcc aactgataag
    11401 attgctagaa gtgtagcagc aagaagacaa caaatagtta gagatgaaat gttaaaatca
    11461 actcaaagat tatcactaga tgctctattt agtcaaatga gtgaaggttc tataaaagac
    11521 cttaatatag ttataaaagc agatgtacaa ggttcagtac aggcagttaa acaatcttta
    11581 gaaaaattat caaatgaaga agttcaagtt aaagtaatac atggtggtgt tggagctgta
    11641 acagaatcag acatattact tgcagcagct tctaacgcaa taataatagg attcaatgta
    11701 agaccagttc caggtgcaga atctctagga gaaaaagaaa atgttgatat aagaacatat
    11761 actataatat acaaggcaat agaagatata caagcggcta tgactggtat gttagaccct
    11821 gaatatgttg atgaagaaac aggaaaagct gaaataagag aaatatataa aatatctggt
    11881 gttggaacag tagctggatg ttatgtgact aatggtaaga tatttagaaa ttgtaaagta
    11941 agattagtta gagatagcat aataatacat gaaggtgagc ttgcagcact taagagattt
    12001 aaagatgatg taaaagaagt taattctgga tacgaatgtg gtatgagttt tgtaaattat
    12061 aacgacataa aagaaggcga tatagtagaa gcttatataa ctaaagaagt agaaagaaaa
    12121 ttataaattc gtatcaaaga aaatcagtta gtaaagaagg tcgatggtat ggcatcatat
    12181 aatagaacaa gaaggatagc agaagaaata agaaaagtcg taagtactat gcttataaat
    12241 ggagtaaaag accctagaat aacatctatg gtgagtgtaa ctgatgtaga agttacaaat
    12301 gacctaagat atgcttatgt atatgtaagt attctaggcg gagatgaaga atccacacta
    12361 acaggtctta aaagtgctgg aggatatata agaagagaag taggaaaaaa tataaaactt
    12421 agatatattc ctgagatagt atttaaactt gatgattcaa tagaaaaagg aatgtatatg
    12481 gatagcctta taaaaagagt taatgaaaaa aatgctcaac aaaatgagga tgaaaattat
    12541 gatgaataat atggatagta ttataaacta tatttatgag ggtgactatt ttatagttac
    12601 ctctcatatc agtccagatg gagacaatat tggttctaca ctttcaatgt attatacatt
    12661 gaagaattta ggaaaaaatg tatattatgt acttgatgat gaagctccat taaatttaag
    12721 gtttttggtt gaaggtgtaa aaatctataa gtctaatgaa tttaatatga agaactattc
    12781 tctaattgca cttgattgtg gggataagat gagaatatgt gtgagtgatg aaatcaaaaa
    12841 taatgcatca aaattaatct gtattgacca tcatgctagt aatgattcat atggagattt
    12901 aaattatgta gatattgatg catcttctac atgtgaatta gtttataatt tattagttag
    12961 atttcagcaa ataaaagata ttaatattat aaatgaagat atagcaacat gtttatatac
    13021 aggtcttgta actgatactg gaaacttttc gtattcaaat gtacatgcaa gtagctttga
    13081 aatggcaaaa aatttacttg ttttaggtgc acaaaaaaat actataatac aaaatatata
    13141 tcaaagcaat tcttctggtt attacaaatt attaggagaa gctctaaagg gtttggagat
    13201 atttgattaa aaaatagtta cgcacactcg tgactttagt catgagttag tagctaaaat
    13261 agtcagcata tagggaaact tatatgtaga gataggata
//
LOCUS       DACXMZ010000096        13280 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.097, whole genome shotgun sequence.
ACCESSION   DACXMZ010000096 DACXMZ010000000
VERSION     DACXMZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13280
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.097"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            74..964
                     /locus_tag="JD016_10840"
     CDS             74..964
                     /locus_tag="JD016_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAU5000132.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            1182..2936
                     /locus_tag="JD016_10845"
     CDS             1182..2936
                     /locus_tag="JD016_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HAU5000133.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKNYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            3321..4307
                     /locus_tag="JD016_10850"
     CDS             3321..4307
                     /locus_tag="JD016_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAU5000134.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     IRPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            4728..5579
                     /locus_tag="JD016_10855"
     CDS             4728..5579
                     /locus_tag="JD016_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009142612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HAU5000135.1"
                     /translation="MKTIRLLYPDYVSGGLEQYFFGANLMSHILPENENQPIVKVEIT
                     PPNRKEKLVTDGIYGKNEVIAGIKNATEKIEKENPDKIITIGGNCLVSQAPFDYLHGK
                     YNDLGIIWIDAHPDVSTVNDDYPNAHAMVLGSLMGYGDNALSSLMKNKKFKADEILYV
                     GLQEIHDYQKKFLDETCVNYKIQTDEFLSDKEILSFIERFENIVVHFDIDVLDENLFH
                     STYFANPDLVGDGSGGGKMTLDKLGNILHCITENSNVVGFTIAEYLPFDEYKLHKLFS
                     KIRMFTE"
     gene            6143..6763
                     /locus_tag="JD016_10860"
                     /pseudo
     CDS             6143..6763
                     /locus_tag="JD016_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321093.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            7008..7154
                     /locus_tag="JD016_10865"
                     /pseudo
     CDS             7008..7154
                     /locus_tag="JD016_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter"
     gene            7176..8657
                     /locus_tag="JD016_10870"
     CDS             7176..8657
                     /locus_tag="JD016_10870"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAU5000136.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKIKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENNENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDKSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            8683..9654
                     /locus_tag="JD016_10875"
     CDS             8683..9654
                     /locus_tag="JD016_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HAU5000137.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEANVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(9910..10512)
                     /locus_tag="JD016_10880"
     CDS             complement(9910..10512)
                     /locus_tag="JD016_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAU5000138.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDSSIEVDNC"
     gene            10793..12004
                     /locus_tag="JD016_10885"
     CDS             10793..12004
                     /locus_tag="JD016_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HAU5000139.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEVELINKISKKYNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYELPK
                     QYYKRKDGYIE"
     gene            12072..12269
                     /locus_tag="JD016_10890"
     CDS             12072..12269
                     /locus_tag="JD016_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAU5000140.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            12303..13274
                     /locus_tag="JD016_10895"
     CDS             12303..13274
                     /locus_tag="JD016_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAU5000141.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
BASE COUNT         5321 a         1503 c         2284 g         4172 t
ORIGIN      
        1 taaaaacaat ttgattggaa ttttcagaaa attaatgcta taattaaaag taaatttaaa
       61 gggaggttat aaaatggcta aattaactct agaacatata aaaggaaata catattatat
      121 tccactccct acaatagtag gtgtatacgt agatggtaag gatgcaattt taattgacag
      181 tggaaataat aaggatacag ctagacaagt tttaagatta ttagaagaac ataatttaaa
      241 tcctaaatta ataataaaca ctcattcaaa tgcagaccat ataggaggaa atgcatattt
      301 gaagaatcaa acaaaatgca agattgctac gactaaaatt gaaggatatt ttacagaaaa
      361 tccaatatta gaatcagcat ttttatatgg tggctatcca agtaaagcac tgaagaataa
      421 atttttatta gcaaaggaaa gtgaagttga ttatattata ccttcaaatg gcaaaattgt
      481 agatacagaa cttgaagcga tttctcttcc aggtcattat tttgaaatga ttggtgttag
      541 aactccagat aatgttttgt ttgttgcaga tagtcttaca ccagagaata taattactaa
      601 atatcatttt ttcttcctac tggatataga ttcacagttt aagacactag aaaaattgag
      661 aatgttagag gctgatttct ttgttccaag tcactctgtc aaaactacag atataaaaag
      721 tttgattaat attaataaga agaagatgga agaaataata gataatatta aaaaggtttg
      781 ttatgagcca gttatgattg atgaagttat tgaaaaaatg tgtgatttgt acaatgtcaa
      841 gcttgatgct aatcaatatg tgttggttgg aagtacaata cgttcttata taacttattt
      901 atatgagaat aatatggttg aatatatatt tgatggtggt aagatgatga taaaagctgt
      961 gtaaatagta ataattttca aattatatga tgaaattgtt ttagtatgac tgttatttga
     1021 aaagtaatat aaaaaaattt atataaataa gctaagatga ttgatataaa gttactgata
     1081 attttaaggt aatatatacg ttactataat attatgttat tatttgtaaa taaggggata
     1141 atttataagc aaattaaaga ccttaattta caggaggaat agtgtatatt atgaataaaa
     1201 aattcaaaaa attcagtaaa aaactaagta tattaatttt agttttgact atgtttcttt
     1261 ttaataattt aactgcaaat gcacagatta aagaagattc aataatagga attgatagat
     1321 atgaaacagc aggacttata gcggatagac aaacttatga tactgtaata ttagttaatg
     1381 gagataaaaa tttatctgat gggctttcat caagtggatt agctggagct attaacgcac
     1441 caatactttt gactaaaaaa agtgaaatac caaaagctac tttaaataga ctagataata
     1501 agagtttaaa tgttgtaaaa aaagtatata taattggtgg ttataatact attgaaaatt
     1561 cagtagaaaa agatataaaa agaaaaggta ttcaagtgga aagaataaat ggtaacaata
     1621 gaattgaaac tagttataat gtagctaaaa aaataaatga agtaagtagt gtaaaagagg
     1681 taatgcttac aaatggattt gtaggagagg ctgatgctat gagtatagca cctgtagttg
     1741 caaaaaataa aggagctata atacttacag atgggaaatc agttccattt aagactgaaa
     1801 gtttaaatgt ttacgctata ggtggcaaat ctgcgataaa tgaggattta gtaaatgaaa
     1861 ctaaagcaac tagaatcgga ggaaatgata gatttgagac caataaaaag gtaatggaaa
     1921 agttttataa tggagcgaca gatttttata taacaaatgg atatcaatta gtagatgcac
     1981 taacattatc tcctttagcc aaagaaaaac caatcgtttt agttgctgat ggaagtaata
     2041 aaggtatttt aaaaggagct aaaagtataa caaaagttgg aggtattgat gccaatatat
     2101 ataaacagtg tttagatatg attaaatata ataatatgga tatgactcca aatattttaa
     2161 aacacttaac ttcagttgaa ggaaatgaag tttctgaaat acttatagaa tcagataatt
     2221 taggtgtggt tgttgaaaag acgaatacca ataaatttga gtttgattac gtaagtgtaa
     2281 ctaatgaaaa aaattgtact ttttctgtat ataaagaaaa ttcttctaat aatataaaaa
     2341 atggaaagct agttgtaagt gctaagaaaa aaatagataa gaaagatagt gatttgcaag
     2401 atatgaatgg cgacaatatg ataaatgcaa ataaagatag gatggtaaat gtgataaaaa
     2461 ttggagttcc cgataaaatg tattctaatt ttaaagtaga tgttgaaagt ggttcagtag
     2521 aactttataa tattaaagga ggagctactg ttgatgtcaa tgacgggatt gctaacattg
     2581 ttgataacaa tgtaacaaat cctttcaata taaatactaa tagtgggatt tcaggtgtaa
     2641 cagctgaaac tatatcatct gaaataaaat ttagagcaaa caatggaatt gtaaatgtaa
     2701 cagctacgaa tataaatggc aatataagtt tatttggaag cgataataaa ggtacctttg
     2761 atggaatatt taaattaaat ctgaaaaaag aaccatcaaa tttacacttg aagttaattg
     2821 gaaatgggat aaataaattg ccagaaggtt ggtctaaaaa ttatatttta ggaaatggac
     2881 aaccaattat tgaagttaaa aataatggca taaataatat atcaattgga gaataaaaac
     2941 atatgaaaat aaagtttata tagttttaaa aattatagaa ttttaaaata tttatatata
     3001 ttctttaaat aaaaaattat tttttaagtg ataattaagt atttatcgat aaggctatgg
     3061 ttgtaaaaat catagcctta tttttatatt ataaataaat taaagtattt atcaattcat
     3121 agtagataaa attgaaatta aatataacaa caaaaaatga aaattacaat taatttaatg
     3181 ttaaaggctc aattctttgg atgtggatat ggatagtaca agtagtatat aaaaagtatt
     3241 gactggacaa ataactatat tttattttaa atatataata taatgtaata aaatatatca
     3301 aatgtagaag gtgataacct atgaaaaaaa taacaataaa tgatattgct aacttggcag
     3361 gagtatctaa aagtactgta tcaagatatc taaataataa agatatcagt gattctacta
     3421 aagagaaaat aaagacaata atagatgaat atggatatga gccgaatgca tttgctcaaa
     3481 gtttgagagc taaaaaaaca tactttattg gtattataac accgtgttta gattcttttg
     3541 taaaatctaa aattatgatg gcaatagatg aagaactcaa agaattaaaa tatacatcac
     3601 taattattaa tactaataga aaaataaggt cagaaataga tagtatatca aagttagcta
     3661 gtctaaaagt tgatggaata atactaattg gtaccgaaat aactaaagaa cataagaatg
     3721 aaattgaaaa gttagatata ccgatagttg tagttggaca aaaagttgat ggtatcaata
     3781 gcatagtgaa tgatgattat ggagcaggat ataaaatggg tcaatatatt gctaataaag
     3841 gttacaaaaa cattgtatat ttaggtgttg atgaaagtga tatatcagtt ggtttaaata
     3901 gaaaaaatgg agtacttaat gggttaaaag ataaagggta tgatgcaaaa gtattttata
     3961 ctgactttga ccaagaaacg tctatacaaa gaagtggaga aatgctagaa agtgaaaatc
     4021 cagatataat aatatgtgca actgataata tagcaatagc aacaatgaaa gaaatcaata
     4081 agagaggtaa aagtatacca caagatatat ctgtagctgg atttggggga tatgatgtat
     4141 tgtctattat aagacctaaa cttacaacaa taaagtttga aaataaaaat gcaggtaaag
     4201 tagcagcaaa tacgatagta aatttaatac aagaaagaaa agaaccacta ttaaaggaga
     4261 ttaaatttga attaatagaa ggtgaaagta caataaataa aaattaagat acacattaca
     4321 tatattagta atgtgtattt tttacgtgaa aaacagatag aaaatcgaaa atatccacat
     4381 aaatacagta tctatcaaca aaacaagcaa aagaaaatac ggaaaattac aacaaatgaa
     4441 agagccttat gtaacgactt ataaagagaa atacaaagat ataagaaaac ggcacttgaa
     4501 cagagtgtct ttttctatgc agtggaacag catactattt catttctgct tgattacctt
     4561 aaaacttatt agtctatggt ttgtcaatgg cttattgtgt aagcaatgtt gaatagcacc
     4621 ctttacaaat catggactgg taagtaactt ttcaaaagca gaaaagaaat gacgataaaa
     4681 atatatcatg ctataattga agaaaattat aattttagga ggataccatg aaaacaatac
     4741 gattattata tcctgattat gtgagtggtg gtttagaaca atattttttt ggcgcaaatt
     4801 taatgtctca tattttgcca gaaaatgaga atcaacccat tgttaaagtg gaaatcacac
     4861 caccaaacag aaaagaaaaa ttagtgacag acggtattta tggaaaaaat gaagttatag
     4921 caggaataaa aaatgccaca gaaaagattg aaaaagaaaa cccagataaa ataatcacga
     4981 ttggtggaaa ttgtttggtt tcacaagctc catttgacta tttgcatgga aaatacaatg
     5041 atttaggaat tatttggata gatgcacacc ctgatgtttc aacagtcaat gacgactatc
     5101 caaacgcaca tgctatggta cttggttctt taatgggata tggggacaat gcgttgtcaa
     5161 gtcttatgaa aaacaagaaa tttaaggcag acgaaatatt atatgttgga cttcaagaaa
     5221 tacatgatta tcaaaaaaaa tttttagatg aaacatgtgt caattacaag atacaaacag
     5281 atgaattttt atctgataaa gagattttgt cgtttataga aagatttgaa aatatagtcg
     5341 ttcattttga tattgatgta cttgatgaaa atcttttcca ttccacctat tttgcaaacc
     5401 ctgatttggt cggcgatggt tcaggcgggg gaaaaatgac attggataaa ttaggaaata
     5461 ttcttcattg cattacagaa aattcaaatg ttgtaggatt tacaatagca gaatatctgc
     5521 cttttgatga atacaaacta cataagttat tttctaaaat tcgtatgttt accgaatagc
     5581 ttatttgatt aacgtggaat atttcaaacc gacggagtgg tccgcccact gtggcggtcg
     5641 taagacatat cgccactggc taggggtggt tcacttcggc ggcttggcaa gcgaagcgag
     5701 ccttataagc cccgttatct aacagggggg ggactattac aagaaacaag agacagaaca
     5761 attacaaaaa tcccgatatg caacgattta taaggttata agcacttact gtacttcgca
     5821 ccttgtcaat tggtggaaaa cagtatatcg acagcaagaa aatacatgat acaaggtgta
     5881 tgctcctatg ggcgtatgcc ttttttgatt ggaggaataa aacatgaatg acattgaatt
     5941 tattgaagca ttgaaagaaa agcggaacgc ctgcggttat tcccaaacaa gactgccaaa
     6001 ggaactgcac atcagcaggc aaaacttaaa ttattcatga agattacgga ctgtttggct
     6061 atgaagaaca atatatctat ggagagatta tcgttacagc ttctaaagac gtttctatgg
     6121 gcgttttacg gaattgaggg gtatgggctg tcggcacttg gaacacgtct tacaggcaag
     6181 gaaaacaggc ttgcgtatct cggagctttg cggactgaca aagaatgatt tggatttcaa
     6241 gaaccatgta atccgtatca atcatcagct acttaaagat aaggacggtt tctacatagc
     6301 atagccaaag acaaagagca gtttccgcaa cgtgcctatg agcgaagaaa cagagaatgc
     6361 ctttcgccga gtgctgaaaa ggaagcagaa gccaacgctc aaagatatag atggctaccg
     6421 caatttccta ttcatcagtc ccaacggaaa ccctatgcaa gaaagcaatt ataaatcggt
     6481 gctgaacggt gttgtaaaga agtacaacaa gaaccatgag aaaacaccgt tgctgaagat
     6541 aacaccccac agcctaagac atacgttctg tacacagctt gtacagaaga acatgaaccc
     6601 gaaaaattta caatatacaa tggggcatgc aagcataacg ataacgctgg acttatacgc
     6661 ctatgcgtca gaggcaggag caaacagaga attgcgaaag catgatagca tgatttacaa
     6721 cgatttgtac aactattgcg agcagaaata taagggatta tgaccagttc cacagtatac
     6781 ctgcaacatg aaaaaaacgc tagaccctta taaacaggga tatattgacg gttcgtgata
     6841 gttataaaaa aatactaaaa aatctatgct gttttttact aaaaaactat aattctaaaa
     6901 attaggaaaa cttttttgaa agaaatattg catctgaaat aaatttatgt taatctttaa
     6961 ttaaaccggt ttcgaaaccg gtttaattaa accgatagaa tagcaaaatg agtgcaaaaa
     7021 taactgattg ttacaaagta tcaaagtaat tattagagta cataggaaat aaataaaata
     7081 taaaaggtgt agcacattgt gtaataagac taagaattgt tttagatagc aataataaag
     7141 caaatataaa attataaaag aaaaggaata aaaatatgta caaagagcct aaatatagaa
     7201 ccatattaga atcgacaaaa gaagaattaa ataaattaag aaatatatct ttaaatgata
     7261 aatataaacc actatttcat atacatccac aacatggact acttaatgac ccaaatggat
     7321 tagcatatta taatggtaag tatcatgtat tttatcaatg gtatccatat gatgcaactc
     7381 atggaatgaa gcattgggct tatgtaagtt ctgatgattt tgtaaactgg aatagagaag
     7441 atgtagcatt aataccaatc gaaagttatg aatctcatgg agcatattca ggtaatgcga
     7501 tagaggtaga tggaaaattg catatgtatt atacagggaa tataaaatat agtgcagaag
     7561 atagatatgc atatcaaaac ttagctataa tgaacaagga tggtaaaata actaaatatg
     7621 aaaataatcc aatagtaagt gaaataccaa aaggatatac aggacatgta agagacccaa
     7681 aagtatttaa aataaaggat aaatacttta tgttacttgg agcacaaaca agtgataaaa
     7741 aaggagttat aatagtatat gaatctaaaa actctataga ttggaatttt aaaggagaat
     7801 taaatgtaaa aaatatagat gaagattttg gttacatgtg ggaatgtcca gattatatta
     7861 atatagatga aaaagatata ttaatatttt caccacaagg agtagaacct aaaggatttg
     7921 attatcaaaa tatatataat gtagtatatg ctattggtaa tatggattta gataatttaa
     7981 catttgaaat agatacaatg aaagaactgg aaaaaggatt tgatttctat gctccacaaa
     8041 catttataaa ggactcacaa ataatactat ttgcatgggc tggaatggga gaagttttat
     8101 accctacaga taaaaacaaa tgggcacatt gtctaactgt tccaagaaaa ttaaatataa
     8161 aaaataataa gctattgcaa atgcctgtag atgaattaat taaattaaga tatgatgaaa
     8221 caagtgggca aaatactata aagaataata tcaatattat agaaaataat gaaaatgtat
     8281 atgagcttaa tattaatata aaaaatattg acagcaataa atttgggctg gaattatttt
     8341 catcacaaga tgaaggtgta aaattagaat ttaacaaatt aggaaacatt gtaactttag
     8401 acaaaagcaa ctttaaaaaa gttttcggtg ttgaatatgg aacaaataga aaagaatata
     8461 taaatattga tgaaaatact aatatcaaag tactagcgga tagaagtatt ttagaaattt
     8521 ttataaatga tggagaagta gtatttacta gtagaatatt tgctaaagaa aattctaatc
     8581 aaataagggt ttatagtgat aaaatagtag agtatgagta tactaaattt aaattaaagc
     8641 aaggtattga attataaaaa cacattataa ggggtagaaa agatgaaaaa agtaattagc
     8701 ataggagaag ctttaataga ctttataccg aatcaaaatg gtggtaaatt aaaaaatgta
     8761 tcagaattta gaagagtagc aggaggagct ccagcaaatg ttagtgctgt tgtagcaaag
     8821 cttggaggaa aatcaagttt tatatctaag ttaggaaaag atgcatttgg tgattatata
     8881 atagatgtat taaatgaagc aaatgtaaat actgattatg tattgagaac aagtaaagca
     8941 aatacaggac ttgcttttgt atcattaaaa gaagatggta atcgtgattt ctcattttat
     9001 agaaatccaa gtgcagatat gcttttagaa gctgatgaag ttaaaaaaga gtggtttaat
     9061 aattgtcata tcctacattt ttgttcagta gatttaatag atagtccaat gaaattagca
     9121 cataaaaaag caatagaata cgcacttgaa agtaatagta taataagctt tgaccctaat
     9181 ataagattac ctttatggga tagtgagcaa tcttgtaaaa aggcaatatc agaattttta
     9241 ccttttgctc atattgttaa gatatctgat gaagaattag aatttataac aggagaaaat
     9301 gatatagaaa aatcattaca taaactcttt gtggggaatg ttgaactagt attatataca
     9361 aaaggaaaag atggagtaga tgcatacact aaaaaagtaa aaggtatgtg taaaggggtt
     9421 aaagtaaatg caatagatac aaccggagca ggagattctt atataggttc atttttatac
     9481 accttattgt ataaacaaat aacattgaat gatataaaag aaatgaaaca agaaacgtta
     9541 aatgagtatt tagaattttc taattattat gcagctcgta gtacaactat taaaggtgcg
     9601 ataagttcat atgcaacaaa ggaagaaata actgaattta taaaaaattt atagtaggaa
     9661 tgagtaatag gttttgaagg aagtaacatg taaagagttt ttaaggatgt ttactatgaa
     9721 aataattaac atataaaaag caataaagag gctgtctcaa aatagagata agcctcttta
     9781 taattgttga aatgataaaa aatattttaa ttattgttac tttttagaag aatatatatc
     9841 ttattttaaa taagcaaatt atgtaagatt tatttatatt atcaatactt tacttgaagt
     9901 attatgtctt tagcagttat ctacttcaat tgaactatct aaaatataat aaatttgttt
     9961 tgcaaattga cgagaatgtt cttctttttc tttattcata aaattaggtt tacctaaatc
    10021 acgttcaaca agcattccta tatattttaa ctgtgaatgc ttacagtagc gcttcatacc
    10081 attttcccaa atatcagcac ccttttcagg tctatatcca catgttgtaa taagtgctac
    10141 tggttttcct gcccatagag cggggccttt ctcattacca tagtatttat tcattccata
    10201 caccaatctg tcaagtagtg ctttcattgg agaagtgcag taccaggaat aaattggagt
    10261 agcaaaaaca attaaatcac acataagaat tttcttgaaa attttctgaa catcatcttt
    10321 acaataacat ccaaaatcaa ataagttatc ctgacatttt ttacaagcaa tacatggctc
    10381 tattttacaa tcatataacc atattacttc aatatttgaa ttataatttt ctaattcctc
    10441 aataaaaggt tttaaaatag cagcagtatt accttttttc ttaggacttc ccataagaat
    10501 gcatacttcc atattgatac ctccacagta ttttaaaata gtttttagtg cttaatatag
    10561 ttttaggata ctatgagaat acgttaacaa caatacactt tgtatatttg taataaaatt
    10621 gaaacaaatt gttttatcaa taaaagttgg aatgtatcta taattaaaaa tattttaggt
    10681 atgtttttac atatatgtgt gtagttaact atggtaattt attgtaaaat tatcttatag
    10741 gattataatt gcttaattta tgattatatt taaaatcttg gaggtaaaaa atatgtactc
    10801 aaatgaagac tataaattta tagaagaaaa tgcaccatgt tatatatatg atgagactaa
    10861 tataattgaa aaatgtaagg aattgaaaaa taatatttta ggttttgatt ttttatattc
    10921 tataaaaaca aatccatata acaaaattgt aaaaagcatt gcagagcaag gttttggagc
    10981 agatgcttct tcaactaatg aagttatgat ttcatcagaa tgtggagttt ctaaagatga
    11041 tatttattat tcatcaccag gcaaaacaga gattgatata gaaactgttt tagataaatg
    11101 cataattatt gcagatagta ttacagaagt agaacttata aataagatat ctaaaaagta
    11161 taatcagaaa gttaaaatag gaataagaat aaatcctgat tttactatgg atgatgatag
    11221 tgctaaatca agcaaatttg gtatagatat agaagatatg agcagtgttt ttaagatgtt
    11281 aagtatgtgt gattatgtat ctgtagttgg gattcatata catattaaat ctcaggtatt
    11341 ggattttgaa aagttaggac attattatga aaaatgtttt tttatagctt taaatttaaa
    11401 taaaagtaag tatattaata ttgaatttat aaattttggt agtggacttg gagtagctta
    11461 tgataatctt agagaaaatc cacttaatct taaagagctt tcaaaattaa ctcaaaagat
    11521 tgcttttact aattcaaaca cactaaaatc aagacttctt attgaatcag gacgtttcat
    11581 agtttgtaaa tcaggtaatt attatgttaa aattgtagat attaaagtat caagagggaa
    11641 aaaatatctt attgtaaaaa atggacttaa tggatttatg agacctatta tttctaattt
    11701 aattgaaaat attacttgtg gagtagaatt agaaggtcaa gagccattat atacatcaaa
    11761 tcatgccttt tctctttcag tattaaataa tttaaaagaa gaagagtatg taaatgttgt
    11821 tgggaatctc tgtacatcat tagatgtaat tgcaaataat gttaaattga aaaaagcaaa
    11881 cataggagat ttgatttcaa ttagtaatgc tggaagttac tcctattctt tatcaccact
    11941 attattttcg agttatgaac ttcctaaaca atattataaa agaaaagatg gatatattga
    12001 gtaaacaata gcataaatta ataaacttag tattattgaa aaaatttaaa atgcaggggg
    12061 tgtaattcta tatggaattg tggaaaagaa atttatttgt atgttggata ggtatgtttt
    12121 ttagttctat agggatgagc caaatagcac ctatacttcc attatatatt aaacagttgg
    12181 gggtaactga tgttagttta atacaacagt attcaggtat aatttttgga tgtacctttg
    12241 tagtagcagc gttcttttcg ccaatttgag ggaaagctgc tgataaatat ggaagaaaat
    12301 caatgctttt acgtgcaagt attgggatgg gaatagttat atttaccatg gcgtttgttc
    12361 aaaatgtcta tcagttatta ggattaagaa tacttcaagg agtttttacg ggttatgcta
    12421 ctgcatgtac tactcttatt gcaacacaga cagataaaaa tcattcagga tgggctttgg
    12481 gtactcttgc aactgcatca actactggct cattaattgg tccaacagta ggaggatata
    12541 tagaatcaat cttaggtctt aaaagtacat ttattataac aggaggattg ttgtttgtat
    12601 catttatcat ctctatttta tttgtagtag ataattttaa acctaaagaa ataaaagaat
    12661 caatttctat acctaaagaa caattaaata tattgccaaa taaattttta attgcatcta
    12721 tttttgtaac cacattcatt actcaactag ctttatactc tatagaacca attgtaacca
    12781 tatatatatc acaacttaca aattttgcat caaatgtagc tttaatagct ggattgacat
    12841 tttctgcatc aggattagca aatttattag cagctcaaaa acttggaaaa atgtcggata
    12901 aaataggtcc tcaaaaagta ttgttaattt cacttttatg ggctgggatt atatttatac
    12961 cacaagcttt tgtaaaaact gcatggcaac tgatgttgtt gagattttta ttagggttgt
    13021 cagttgcagg gcttaatcct tctgttaaca gcttgcttaa aaaaattgca ccagaagaat
    13081 atgtgggaaa aatatttgga tataatgcat ctgcccagta tataggctgt tcatcaggag
    13141 catttttagg gggacaaata tcagcacatt taggtattag aacagtattt ttttcaacaa
    13201 gtttattgtt gtttatgaat gcactttgga tgtataaatt tactggattg tttattaaag
    13261 ataagactaa ataatcatac
//
LOCUS       DACXMZ010000097        13262 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.098, whole genome shotgun sequence.
ACCESSION   DACXMZ010000097 DACXMZ010000000
VERSION     DACXMZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.098"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            70..1521
                     /locus_tag="JD016_10900"
     CDS             70..1521
                     /locus_tag="JD016_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HAU5000142.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1743..2204)
                     /locus_tag="JD016_10905"
     CDS             complement(1743..2204)
                     /locus_tag="JD016_10905"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAU5000143.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(2309..3502)
                     /locus_tag="JD016_10910"
     CDS             complement(2309..3502)
                     /locus_tag="JD016_10910"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HAU5000144.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(3525..4190)
                     /locus_tag="JD016_10915"
     CDS             complement(3525..4190)
                     /locus_tag="JD016_10915"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAU5000145.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(4224..5336)
                     /gene="ribD"
                     /locus_tag="JD016_10920"
     CDS             complement(4224..5336)
                     /gene="ribD"
                     /locus_tag="JD016_10920"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAU5000146.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(5468..5589)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5778..8261
                     /gene="recQ"
                     /locus_tag="JD016_10925"
     CDS             5778..8261
                     /gene="recQ"
                     /locus_tag="JD016_10925"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAU5000147.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYAKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      8350..8461
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8603..9904
                     /gene="thiC"
                     /locus_tag="JD016_10930"
     CDS             8603..9904
                     /gene="thiC"
                     /locus_tag="JD016_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAU5000148.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            9958..10152
                     /gene="thiS"
                     /locus_tag="JD016_10935"
     CDS             9958..10152
                     /gene="thiS"
                     /locus_tag="JD016_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAU5000149.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            10145..10948
                     /gene="thiF"
                     /locus_tag="JD016_10940"
     CDS             10145..10948
                     /gene="thiF"
                     /locus_tag="JD016_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAU5000150.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            11011..11781
                     /locus_tag="JD016_10945"
     CDS             11011..11781
                     /locus_tag="JD016_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAU5000151.1"
                     /translation="MDKLILGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            11798..12901
                     /gene="thiH"
                     /locus_tag="JD016_10950"
     CDS             11798..12901
                     /gene="thiH"
                     /locus_tag="JD016_10950"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAU5000152.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            12902..>13262
                     /locus_tag="JD016_10955"
     CDS             12902..>13262
                     /locus_tag="JD016_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAU5000153.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELKK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNE"
BASE COUNT         4947 a         1585 c         2247 g         4483 t
ORIGIN      
        1 gaattattca tttttttgat tttaagattt attgagaaaa caaaactaaa taggggggta
       61 attaatataa tggatttttt caatggtatt ttaacgagta ttaacaatgc agtagtatat
      121 gtaaacaatt acttatggtc gtatatctta attataatgt tagttgtaat tggtatttac
      181 tttactatta aaacaaattt tgtacaattc agatatttta tagaaatgtt tagacttctg
      241 ggtgatggta cagcgaataa aaatgcaaaa aaggaaggaa aaatctcttc atttcaagca
      301 ttttgtataa gtacatcttc aagagttgga actggaaata tagctggaat agcaattgca
      361 gtggtagcag gaggacctgg tgctgtattt tggatgtggc ttatagcatt aattggttct
      421 gcatcaagct ttgttgaaag tacattagct cagatttaca aagtgaaaga tggtcaagct
      481 tttagaggtg gaccagcgta ttatatggaa caaggtctaa ataaaaaatg gatgggtatt
      541 ttattctcaa tacttataac tatatgttat ggttttgtat ttaatgcagt tcaagcaaat
      601 actgtttcac ttgcatttaa taatgcattt ggaataagta aaatggctat gggaataggt
      661 cttggaattt taactgcaat agttattttt ggtggagtgc atagagttgc aaaagtttct
      721 gaaataatag ttcctgtatt tgcaggatta tatatattag ttgctttggc tatagttgta
      781 atgaatataa cagagatacc aagtgttata gcattgatag ttgaaagtgc atttgatttt
      841 aaaggaatgg cgattggtac atttatggga gttgtaatga ctggagtgaa gagaggtcta
      901 ttttctaatg aagcaggtat gggtagtgct cctaatgctg ctgcaacagc acatgtaact
      961 catcctgtaa aacaaggttt aattcaatct ttaggtgttt ttacagatac tataataata
     1021 tgtagttgta cagcattcat ggtattatta tattcaggtt atagtagtag tggagcaact
     1081 ggaattgaat tagctcaaga agctttaact catcatatag gaccagttgg aaatatattt
     1141 atagcagttt gtatattctt gtttgcattt agttctatag ttggtaatta ctattatggt
     1201 gaatcaaata tggagtttat gagtggaagt aagactaaac ttaatgtatt tagagttttt
     1261 gttgtaggta tggttttatt tggttcactt actcaagtag atgtagtttg gaatcttgca
     1321 gacttattta tggcattgat ggcgataata aacttaatag caatagcact tttaggaaaa
     1381 tatgcattca ttgctttaaa agattataca agtcaaaaaa aatcaggaat aaaagaccct
     1441 gtgtttattg cagatgaaat agaaggccta gaaaaggtat cagagtggca tagggaatta
     1501 tctgaggaac atttaggata aaaaatagta taaaattata aaaccatttt gaaagactag
     1561 agtatgtata tactttatat gaatataata ttgaattaat ttatattact ggaattttta
     1621 attccagtaa tattttttta ttatatataa tttagcttta ttaaaagttt ttattattaa
     1681 aaatagctta tttatcgtaa agtgaatgat attgagtttt taattattat gaatgttagt
     1741 atttaattta aagacttaaa taaatttgcc atttctagcg cattcatagc acaatcataa
     1801 cctttatttc cagcttttgt acctgctctt tcaatagctt gttcaatatt ttctgtagtt
     1861 acgattccaa atataacagg taattcactg tctaaagata ctttggctat ccctttagag
     1921 acttcactac atacataatc ataatgagaa gtagcccctc ttataacaca gcctaaacag
     1981 attatagcat catacttacc acttttagcc attttttgag caacaagagg aatctcaaaa
     2041 gctccaggta cccaaactat ttctatattt tctggagaaa cgtcatgtct taaaagacag
     2101 tcttcagctc ctgataaaag ttttgatgtt ataaattcat taaaacgaga atttattata
     2161 cctattttta aatcttttcc aataagtttt ccttcatata tcatttttat ttcctccaat
     2221 tttatttatg ttatagattt atatttataa atatctttaa tttaattata aaaaactact
     2281 tagtataaaa atatcatcat ataatcattt atataatatc taaaagatgt cccattttat
     2341 cttttttagt ttttaaatat agcttattaa agtcattaat ttcttcatta atagggattc
     2401 tattttcgat ttttattcca tatttctcta attgatttat tttatctggg ttattactta
     2461 aaagatttat actttttatg tttaaatttt ttaatatttg agcagccata tagaaatctc
     2521 tcatatcttc ttcaaatcca agcattaaat ttgcttcaac agtatcgtaa ccttcttctt
     2581 gaagtttata agccttaatt ttatttaaaa gaccaatacc tctaccttct tgcttcatat
     2641 aaattaaaac tccacttcca ttttccacaa ttttcttcat agaagattct agctgtaatc
     2701 cacaatcaca ttttagagag tggaacacat ctcctgttaa acattcagaa tgaagtctaa
     2761 ctagagtatt ttctatattt atatttccat aagtaagggc aatgtgttct tcatttgaat
     2821 aagtatctct atatcctatg atttcaaaat ttccatattt tgtcggaagg aaagcactag
     2881 atactttttc tatagtagtt tcatttattt tgcggtattc tataaggtct tttatagtga
     2941 tgatttttaa attatgcttt tctttgaaaa tcattaaatc atcaactctt gccattgtac
     3001 catcatcttt cattatttca cagataagac caatctcttt aaaacctgca agtctagcaa
     3061 ggtctactga ggcttctgta tgtccatccc tgacaagaac tccaccagtt tttgctatta
     3121 aaggaaagat gtgaccaggc gttttgaaat ctttacttgt agaattttca tcacagaata
     3181 attttacagt ttcagctctt tcatatgcag atataccagt tagagtatct gctccatcta
     3241 tagtaacagt aaaggctgtt tgaaatgtat ctgtattttc tctaaccata agaggtatat
     3301 taagactttt aaattttttt tcagtggcag gtagacaaat tagacctcta ccatatgtag
     3361 ccataaaatt tattgattca ggagtagcca tttcagctgc catcaataaa tctccttcat
     3421 tctctctact ttcatcatca actacgatta ccattttccc attttttata tcttcaatag
     3481 cttcttcaat agtattaaac atgtaattca ctcctttatt atttttaaaa accattttta
     3541 aataaaaaat ctttatctat atttgattta tggttactac tattagaatt taattccttg
     3601 tagttattag ccataaaatt attaacatac tttccaatta catcgctttc taaattaacg
     3661 aaatctccaa cattctttgt caagagaata gtgttaattt tagtatgagg tataatagaa
     3721 accttaaata tttcatcatc taaatatgat atagttaaac ttacaccatc aatagcaaca
     3781 gaacctttga gtatcattga ggataaaagt tttttatcag gtcgtatact tactaaaact
     3841 gcattttcat ctttttcaaa tttgcagata gttcctttac catcaacatg acctgttact
     3901 atatgaccac caaatcttga agaaagactc atagctcttt caagatttac cttgtcattt
     3961 gcatttaata aacctaaatt tgtggaacga attgtctcca tcataacatc ggctgtaaat
     4021 tcattttttc cgaggtttga aacagttaga caaactccat ttacggctat actatctcca
     4081 agatttgtgc catctaatat tttatttgat agtatggtta cttttccact cttaccatta
     4141 gcagtaattt ttctaagtat tccaacttct tctactatgc cagtaaacat cctaacctcc
     4201 tatatgaatg tattaacaaa aatttaattt aaaacatctg attctataag aatatcgcca
     4261 tctatcaaag ttacattatt gattgttaac tgtgttgaat ctgacaaatg atttattcct
     4321 tctccagaaa cgaatgtagg tgcatcttta ccacctataa tcttgggtga tagaaataat
     4381 ttgactttat caactaactt atttctaaag agactgtcat tgagagtagc accaccttca
     4441 actagaacag aatcaatgtt taactctcct aatttataca ttacaaactc caaatctact
     4501 aagttgtttt ttgaaggaga aattattgtt tcaacattat tttcttttaa catagataat
     4561 tttatacttt ctttgcaaca tgtgaaaact atagttttac tggttttatc tttaaccaaa
     4621 ttactattaa gtggcaactt taggtgtgta tctattacta ttcttgtagg atgagacact
     4681 ttttcttgat tgagcctaca agttagtaaa ggattatcgt tcaaaactgt atttatgcca
     4741 accattatac tttggtattt atttctgtat ttatgagtta gataccttga tgattcatta
     4801 gatatccatt ttgattctag tgattttgtg gcaattttac catctagact tactgctgat
     4861 tttacaatac aaagaggtct tttgtttttt atatgataaa aaaatgcctc atttaaatct
     4921 cttgcttcat cttctaatat tcccacagta acatcaatat tgttatcttt taattttttt
     4981 actccattac cacaaacaag tggatttggg tctaatgtag atattacaac tctcttaatc
     5041 ttattttgta taattttatc tacacaagga ggcgttttcc cataatgaga acatggttct
     5101 aagtttacat aaagagtaga gtcttttaga ctttgtttag cactgtttat agcatttact
     5161 tcagcatgat tagagccaaa tttctcatga tatcctttgc ctattatatt actatcttta
     5221 acaattacac atccaactaa tggatttgga tttacaaagc cttctccatt tttagccaat
     5281 tcaatagctt ttttcatata ataaatatct ttttcttttt gattcacaaa attcacaata
     5341 tgtacacctc ctatttaatt ggataatttt taaacaaaaa accctgagaa ttaactcagg
     5401 gtttagaata atctacaata aatgtttaat aaatgtagaa caaaattgct ctacaaaaac
     5461 actcattaaa tatcttcttc catccagact ttactgtcgg ctttggaatt taaccaaatc
     5521 aaccaagttt taccttggct cgcgggctat accgccgatt gggaatttca ccctaccccg
     5581 aagattaagt attaaattta tatttagaat gtatcataaa ttattttact ttgcaaacta
     5641 tttttttaaa aaatatatgt taatatataa tatagaatgt ctataaactt tgaaatacca
     5701 cgattgtgaa tataaaaata aaaatttgaa cataatcagg aaagaagtct aattaattta
     5761 aattaggaga gtaatatatg aataaaaaac ttttagaaat actatcaaaa tactatggat
     5821 atacaagttt tagaaaaggt caggaatcta taataaattc tatcttaagc gaaaaagatg
     5881 tgctagctat tatgccaaca ggagggggaa aatcaatatg ttatcaaatt ccagctttga
     5941 ttttagatgg aatgacaata gtaatttctc cacttatatc acttatgaaa gaccaagtag
     6001 atgccttaaa gactatggga attagtgctg cttttataaa tagttcatta agcagcaaag
     6061 aatttaatga aatattaaat aatattagaa aaaataatta taaaatactc tatattgctc
     6121 cagagagatt agatgcacaa gagtttattg agctaataaa taataacaat gtaagtcaag
     6181 ttgcgataga tgaagcacat tgtgtatctc aatgggggca tgattttagg ttgagttata
     6241 gaaggatttc tgattttata aaaaatttgc ctaaaagacc tatagttaca gcatttactg
     6301 caacagcttc agaagaggta agaactgata taataaattt actgtgttta gaaaatccag
     6361 attattatat aactggattt gatagagaga atttatcaat aaatatagta aaatcatcta
     6421 gtaaaaataa gtatatttta gattatattc aaaatcataa aaatgagtct ggaattatat
     6481 atgttgcaac tagaaaggaa gttgaaaata tatataatgg tttatctaag agaaatatat
     6541 ctgtgtcaaa atatcatgca ggtttatcta aaaatgaaag aagtaaaaat caagaagatt
     6601 ttataaatga taatgtggat ataatggtag caacaaatgc atttggaatg ggtattgata
     6661 aacctaatat aagatgggtt atacattaca atatgcctca aagtatagaa aactactatc
     6721 aagaaatagg tagagctggt agggatggag aaaaaagtga gtgtgtactt ttattttctc
     6781 caggagatgt acatatacaa aaatatttaa ttgatattgg agttgaaaat cctgaaagaa
     6841 aattatttca gcataagaag cttcaataca tgattgactt agtttacagt aattcatgct
     6901 atagaaagac aatattgagt tattttggag aaaattatat agacaattgc aataattgta
     6961 gcaattgttt agtagagggt gaaatagtag ataaaactat agatgcacaa aaagtcattt
     7021 catgtgtagc tagaatgaaa agaagttatg gtgttactac tattgtagat gtgcttagag
     7081 gttctaagaa taaaaagatt ttacagcttg gattaaatac tttgtctaca tataacatta
     7141 tgagagatta ttctagtgaa gacttaaaga attttgtaaa tacattggtt tctcatggtt
     7201 ttttagattt agttgagact ctaggcaatg gaaatagagg tagttttcct actataagat
     7261 taaatgagat gtctatgaaa gttttaaaag gtgaagtgaa agtagagttt aaagaaattg
     7321 ttatgacaaa atcattggaa gtagaggatg agttatataa tgctttaaga gaacttagac
     7381 attcaattgc aagtgaagaa aggatagctc catatatggt atttggagat ggtacactta
     7441 gagccatgtc tagtagttat ccaattaata aagaagaaat gctagatatc tctggagtag
     7501 gagaaattaa atatcaaaaa tatggtagag attttgaagc agtaattaaa gaatatgtcg
     7561 aaaaaaataa tataaataaa aaaatagatg aaaaaaatgt cgattcaaag aacgttaata
     7621 gtgatttctt agaggttaat acagataaag ttttatatga aaaactgtta gtcattagag
     7681 aagaatatgc aacaaaggaa aaattacctg catatatgat tttagccaat aaggctttga
     7741 aagaaataag tggaagatat ccacttgatg aagaacaatt aaaagatatt tctggcatag
     7801 gtggagtaaa atttgaaaaa tatggagcta gtattttggc agaggttaat aaatatgtaa
     7861 aagaaaataa tattaatgtt atttgggaga aaaagggcaa aaggaagtta atattagatg
     7921 gagaaagtag aaaaaataat gaaatagcat tagatttgct aaatcaagga aaagatatac
     7981 aaagtatcag tcaagaaata gaagtttcac tgtcaaccat tattggatat gtgcatgact
     8041 atgcaaagga tggacatagt atatcatttg aattaggact agaaaatttt tataatgaag
     8101 ataaaaagaa gatgatttta gaagtttgcg aaaaagttgg ctatgataat ttaaataaca
     8161 taaaaagaaa acttccagat tcaataaagt atgaaaatat aagagctgtg atacttgact
     8221 cttatcttga aaacttaaaa aataatatta tagcagagta atttttaact taataaaaaa
     8281 taaaatttgt attattgtgt tgaactaata attttgctgt gttatactga atttgtaaaa
     8341 ataatgaaaa attagagcta ggggcgctag aattggctag ctgagagata aagccgtaac
     8401 ttacaaaccc taataacctg atcaagataa tgcttgcgaa gggaagcttt ttaatataat
     8461 tttattatat aaataggcaa attattcaat gttaggagaa aattgtctaa gtgtatatac
     8521 gattataaga gtgctttcca aacgaggaaa agcacttttt ttattttcaa aaactataac
     8581 tttaatcttg gaggattgta atatgaacta tacaacacaa atggatgctg ctagaaaagg
     8641 cataataact aaagaaatgg aaatcgtttc tcaaaaagaa caggtagatg taaatgaatt
     8701 aagagaatta atagcaaatg gacaagttgt tataccagct aataaaaatc ataaatcttt
     8761 aagtgcagaa ggtgtaggta aaaatttaag aacaaaaata aatgtaaatc taggaatttc
     8821 aagagattgt aaggatatag aaaaagaact tgaaaaagtt agagtagcaa tagacatgaa
     8881 agctgaagct ataatggatt taagtaatta tggcaaaact agagaattta gagaaaaggt
     8941 tgttgaaatg tcacctgcaa tgataggttc agtacctatg tatgatgcag taggatattt
     9001 ggaaaaagaa ttaaaagata taacagaaga agagttttta aatgtaataa gacaacatgc
     9061 tattgatgga gtagacttta ttactataca tgcaggtctt acaagaagtg tttgccaaaa
     9121 aataaaaaat catgagagat tgactcatat agtatctcgt ggaggttctt tattgtttgc
     9181 atggatggaa ttaaataata aggaaaatcc tatttatact aattttgata aaatattgga
     9241 tatatgtgaa gaatatgatg ttacattgag tttaggagat gcttgtagac caggttgtat
     9301 aaaggattct actgatggtg ttcaaattca agaacttgta gtattaggtg aacttactaa
     9361 aagagcatgg gaaagaaatg tacaagtaat gatagaaggt cctggacaca tggctattga
     9421 tgaaatagaa gcaaatgtag tattagaaaa gagattatgc catggagcac ctttttatgt
     9481 tcttggacca ttggttacag atatagcacc aggttatgac catataacaa gtgcaatagg
     9541 aggagcttta gcatgtgcta aaggagtaga ttttttatgt tatgtaactc cagcagaaca
     9601 tttaagattg cctaatttag atgatatgaa agagggtata atagctgcaa agatagctgc
     9661 tcatgcagga gatatagcta aaaatgttaa aggtgctcgt gaatgggata ataagatgag
     9721 taaagctaga gctgatttag actggtgtga gatgtttaga cttgcaatag accctgaaaa
     9781 agctaagaga tacagagatg aatcaactcc tactcatgag gatagttgta ctatgtgtgg
     9841 aaaaatgtgc tcaatgagaa cagttaagaa aatattaaac aatgaggaac ttaatctaat
     9901 ataatttttg gctagtatga ctggaataaa aagaagctga aaagaggagg tgatttagtg
     9961 aaggttaatg ggaaggaaat tgaatttgaa aaagatttga ctgttataga tttattaaat
    10021 aaatataatt taaaaagtga tagagttgta gttgaagtga atttagagat aattgaagaa
    10081 tctaactaca atacatatgt attaaaagac gaggacattg tagagcttat tagctttatt
    10141 ggaggtggtt aaaataaaaa tatttgtaaa tgaattatta acagatgttg aggaaaatac
    10201 aactgcttat aaagttagag atagctataa caataaatgt gatgtattgg tgttaaatgg
    10261 atatccaatt aaaacagata tgcccctttg tgaaaatgat aaattaacac ttatacaaaa
    10321 aggagttaag ccatcacttg atgaattaga aagcttgttg attgctagac atactccaaa
    10381 tatacataat aaattaaaaa aagggaaagt tgctatattg ggattggggg gacttggttc
    10441 caatatagct atctctttag ctagaatagg agtgggagaa cttttactta tagattatga
    10501 tgttgtagaa ccatctaatt taaatagaca gcagtatttt atagatgata taggaaaact
    10561 aaaaactgat gccatgatag aaaatataaa aaaaataaat ccatttataa aattaaataa
    10621 acgagatatt tttttaaaca agaacaatat ggatactatt aaggattcag atttaataat
    10681 tgaggccttt gatgatgctt catgtaaagc acaggtatgt aattatgtat taataaattt
    10741 aaaagataaa tatttaatag catcatctgg tatggcaggt tactatgatt caaatataat
    10801 aactactaag aagataaaag acaggtttta tatatgtgga gattttgtga atgaagctaa
    10861 atttggtgaa gggcttatgg ctccaagggt tgccatctgc gcaaatcata tggcaaattt
    10921 ggcaacgcaa atacttatag atatgtaaat attaaaatta gatataaaaa agattgaata
    10981 ttaaaaaaat ttaacgtgga gggaaataat atggataaat tgatacttgg tggacatgaa
    11041 tttaatagca gattattggt tggaacaggt aaatatggtt caaataatat attgcctgag
    11101 gtaataaaag aaagtggaag tgaaataata acaatggcac ttagaagagt ggatttagac
    11161 aataaacaag aaaacatatt aacgtatatt ccaaaagaga tgacaatact tccaaataca
    11221 tcaggagcta ctaatgcaga agaagctgta agaatagcta gaatatctag aaaaatggga
    11281 tgtggagatt ttataaaaat agaagtaata tcagatacta gatatttact tcctgataat
    11341 gaagaaacta taaaagctac aaagatactt gctgatgagg ggtttatagt tcttccatat
    11401 atgacaccag acctttatgc tggaagaaga cttatagaag ctaatgcagc ggcagttatg
    11461 ccacttggag ctccaattgg ttcaaataga ggtcttcaaa tgaaagaaat gataagaata
    11521 atgatagatg agttggatat accaataata gtagatgcag gtattggaaa accatctcaa
    11581 gctatggagg ctatggaaat gggagctgat gcagtacttg tcaacactgc tatagcttca
    11641 gcaggagacc ctgtacaaat ggctagagca tttaaattag ctgtagaagg tggaagagaa
    11701 gcatatattg caaaaacagg aaatgtgtca gaatttgcaa atgcatcttc tcctttaaca
    11761 ggttttttag gcaatttata gaaagtggtg attggtgatg agtttttatg atgtaataga
    11821 aaaatatagg gattttgatt ttgatggata tttaaacaat gttactgata atgatgtttt
    11881 aagaagttta tcaaaagaca agcttgaaga ttttgatata ttaaatttgc tttctaaaac
    11941 tgcagtaaaa catttagaag atatggcaca aaaagcacat aaattaagtg ttcaatactt
    12001 tggtaaaaca gtatgtttat atacaccaat gtatatagca aattattgtg ttaatcaatg
    12061 tgtttattgt agctataata taaagagtgg aataaaaaga aaaaaattaa ctatggatga
    12121 aattagagaa gagggagaag caatttcaaa agaaggtttc aaacatctac ttgttttgac
    12181 tggagaaagt agttttcact caagtgtaga atatataggt gaggctatag aaatattaag
    12241 agagaaattt ccctctatag gtatagaagt ttatccaatg gaagtagaag aatataagta
    12301 cattgtagat aaaggtgttg aaggattaac agtatatcaa gaaacttatg acgaggaaat
    12361 atataagaga gtacatataa aaggcccaaa atcaaattat aaatttagac tagatgctcc
    12421 tgaaagaggg gcaaaagcag gtatgaggac tttatcaata ggtgcacttt tagggctaaa
    12481 tgattttaga aaagaaactt tttttacaat attgcatggg aaatacttaa aaacgaaata
    12541 tcctcatatt gagctttcat attcaacacc aagaatgaga ccttttaaag ggtgttttga
    12601 ggaattggta gatataagtg atactgattt agttcaagct atggtatgta tgagattatt
    12661 tgaccctcat gcagctataa atatatccac tagagaaaat ttagaaatga gaagtcatat
    12721 aataccttta ggagtaacta aattgagtgc aggtgtatct actgatgtag gaggtcattc
    12781 tcaagatgaa catgatactg ctcaatttaa gataaatgat gaaagtactg ttaaagatgt
    12841 ggagaaaatg ctaaactcaa ttggttatca gcatgtgttt aaagattggg aaagatttta
    12901 aatgtatctg ataacaaaca gaaaattatg tagtgaggaa cgatatttag aagttattaa
    12961 agagtctata ttgagtggtg tagaaaatat aataataaga gaaaaggatt tggaatatca
    13021 agagctaaaa aaattatata tgaaaataaa aactaaaatc aattgtatag attttcaaga
    13081 gcaaatatca gatgaaagtt tgaaaactaa tataaatcaa aaagagtgta gaaataaatt
    13141 caaggttaat tttataataa atagtaacat tgaatttttt gaaaaagtgg attgtcaagg
    13201 tatccatctt ccttttaaat tattcttgaa tttaattgaa aataagtata attttaatga
    13261 aa
//
LOCUS       DACXMZ010000098        13260 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.099, whole genome shotgun sequence.
ACCESSION   DACXMZ010000098 DACXMZ010000000
VERSION     DACXMZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.099"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            163..1239
                     /locus_tag="JD016_10960"
     CDS             163..1239
                     /locus_tag="JD016_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAU5000154.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1342..2184)
                     /locus_tag="JD016_10965"
     CDS             complement(1342..2184)
                     /locus_tag="JD016_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAU5000155.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2368..2940
                     /locus_tag="JD016_10970"
     CDS             2368..2940
                     /locus_tag="JD016_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAU5000156.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3103..3375)
                     /locus_tag="JD016_10975"
     CDS             complement(3103..3375)
                     /locus_tag="JD016_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000157.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3489..4361)
                     /locus_tag="JD016_10980"
     CDS             complement(3489..4361)
                     /locus_tag="JD016_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAU5000158.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4657..7764)
                     /locus_tag="JD016_10985"
     CDS             complement(4657..7764)
                     /locus_tag="JD016_10985"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAU5000159.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVEALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7857..8089)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8264..8791)
                     /locus_tag="JD016_10990"
     CDS             complement(8264..8791)
                     /locus_tag="JD016_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAU5000160.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8826..9611)
                     /locus_tag="JD016_10995"
     CDS             complement(8826..9611)
                     /locus_tag="JD016_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAU5000161.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYERAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9621..9878)
                     /locus_tag="JD016_11000"
     CDS             complement(9621..9878)
                     /locus_tag="JD016_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAU5000162.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9897..10355)
                     /locus_tag="JD016_11005"
     CDS             complement(9897..10355)
                     /locus_tag="JD016_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAU5000163.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10395..11108)
                     /locus_tag="JD016_11010"
     CDS             complement(10395..11108)
                     /locus_tag="JD016_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAU5000164.1"
                     /translation="MIIMGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVD
                     LVNEAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSL
                     DRIALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKI
                     KGLMTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEG
                     ATIVRVGTSIFGERNYNKK"
     gene            complement(11109..11744)
                     /locus_tag="JD016_11015"
     CDS             complement(11109..11744)
                     /locus_tag="JD016_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000165.1"
                     /translation="MKKVKYTRLVILGILIYLVFNTLVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12125..13165
                     /locus_tag="JD016_11020"
     CDS             12125..13165
                     /locus_tag="JD016_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAU5000166.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
BASE COUNT         4228 a         2262 c         1563 g         5207 t
ORIGIN      
        1 aaagtcattt tacttccgta tttaatgcaa aagtagagat taaacttaat ataaaaaata
       61 tataacaaat tttttttata tttctagaag aaaagtatac tcttttttgt ttaataatgc
      121 taaaattgat ttgtattgaa ttttgattag gaggaaatta ttatgaaagc tcaaagatta
      181 aatgctgtac ttgaacaaat gaaaaaagat gatatttctc aaatgttagt aagtgaccct
      241 acttctatat tttatcttac aggagtacta atccatccag gcgaaagact attagcatta
      301 tacttaaatt taaatggcaa taataaatta tttataaatg aattattccc agtttcagaa
      361 gatttaggtg ttgaaatggt ttggtttaat gatactcaaa atccagtaga aataataact
      421 gaacatatag acaaaaatgc tactatgggt gttgataaaa actggccagc tagattctta
      481 ttaaacttaa tagaattagg tggaggaagt aagtttgtaa attcatctta tataatagat
      541 actttaagaa tgtgtaaaga tgaagaagaa aaagagttaa tgagaatagc ttcaaaatta
      601 aatgacaagg ctatggaaca acttaaagct actgtttctg gtgaacttac agaaaaacaa
      661 cttgttggta aattatctaa aatatatgaa gacctaggta ctgatggatt ttcatttgac
      721 cctattatag gttttggacc taatggagct aatccacatg gtgaacctgg aaatgcactt
      781 gtgaaaccag gagatgcaat aatcttagat ataggttgta taaaagataa ctattgtgct
      841 gatatgacaa gaactgtatt ctataaagaa atacctgaaa aaggtagaga aatatttgaa
      901 atagttcttg aagctaataa gagagctgaa gctattgtaa aaccaggtgt tagattctgt
      961 gatatagatg ctgctgctcg tgactacata actgaaaagg gatatggaca gtacttcact
     1021 catagaactg gtcactctat aggacttgaa gttcatgata aaggagatgt atcttctata
     1081 aatacagata ctgtacaacc aggaatgata ttctctatag agccaggtat atatttacca
     1141 ggtgagttcg gagttagaat agaagattta gtacttgtta ctgaagatgg ttgtgagata
     1201 ttaaacaagc atgacaaaga aatatgtgta gttggttaat tctaaaatca atttgataaa
     1261 attaaaaagc catataaaaa tgttttaaac atttttatat ggctttaatt tctcaatcta
     1321 tgatttattt caatccatta tttatattgc agttattaag attgcaccct ttgaagtata
     1381 tgtccccatc actgaaccaa tttcacagat tacaacatct ttgtatttta catccttttc
     1441 cataacttcc cttacctttt gagccttttc tgggcaatta gcatgtccta taaataaaac
     1501 cttttcttct ttatctttaa tttttgactc tactaaagta atcagctttt tcaaactgtt
     1561 tccttcacct cttgccttat ctataggagt tacaacgcca tctgcaactt gtattatcgc
     1621 cttaaaattt aaggtattta taatttttcc tgctattgga tttattctac cacccttaat
     1681 agcattttct aaagtttcaa gtgttccaaa aaatggagga ttatctttat atttatttat
     1741 tattgaaaca atctcttcaa tttcttttcc ttcttttatt aatattgctg cttttaacac
     1801 taattgtcct attccaattg acccactaag tgaatctata acctcaactt tcttgttatt
     1861 tccttccttt tcaaacatat cttttgctaa aagtgcacta ttataagtcc cagataatcc
     1921 tgaacttata ttaatcacta atacactttc ttcctgacaa tggtatgctt ctaaaaattt
     1981 atcaggagaa ggacatgatg tttttggtag tactttactc tcactcattt ttctatagaa
     2041 agtttcatta tctatttcct ttccagatat ataactttct tctccaaaag atacatttaa
     2101 accaacaact tctatgttgt atttttctat tatatcatca ggtaagtcac atgcactgtc
     2161 cactatcaat tttattttat ccatattaac ctcactataa gatattattt tatttattca
     2221 gtcggtcgta cgaccgattt aattattatt ttatatattg aatgaaaaaa agtcaataga
     2281 tatagattct cgctttattg acaataaata tccataagct tataatgtta ttaatttgtt
     2341 ataaatatac atggaaaggt aggttttatg aaaacaaata atatgaaaca ggctatttta
     2401 tcatctgcaa ccaatttaat agccaaaaat ggagttcaaa atactagcct tgctgatatt
     2461 gcaaaagatg taaatataag taaaggtact ttgtattatc actacgcatc taaagatgat
     2521 attatatatg atattgcgga tactcattta gaagttataa ctagtgctat actaaactgt
     2581 gtaaaaaatg tcaaatcaaa aaattctcaa atagaaatgg ttaacttaat cttagagaaa
     2641 atatctacta tagaaagtcg tggtagggta catatgtacc ttatttgtga ggcaattact
     2701 tcaaataatg atttaaagga aagaataaga ttaaaatata ttgaatggag aactactctt
     2761 aaatctgaaa ttgttgaatc actagaaaaa tataatagta gcgaaaatga acaggatgct
     2821 gaatcatttt cttttttact catgtcaata gttgatggtt tagttgtcca aagtttatta
     2881 aaaacagaaa aaatacctta tgaaaatata gcttcttttt tagttaatca ttggatttaa
     2941 acttaatact tagataatta ataaaagtga actatttaag ttatctactt taacagttca
     3001 ctttcttatt tattctcaat aatttttact taaaacattc aatttttatt taaaccttca
     3061 tggaatcaca aaataaaaat ctcttataaa tttaaaatgt ttttatttat gttcatgtgt
     3121 atgcccatct gaatgttcat gaccatgtgt atgtgtatgc tcatggctat gctcatgagt
     3181 acctgaaaaa ccatcatcat gattatggtc gtgacctaca ccatttacat gctcatgttc
     3241 atgcccatga ctgtgagaat gttgatgtga atgttcatgg ttatctcctc cacagcatcc
     3301 ttctcctcca gtcccacaac acccacttgt atgtggatta tgatgatttc ttactacaaa
     3361 tctctctctt aacataattc ttatacctcc tagtatatta ttgagtactt catgtacccc
     3421 attaactaca gtatacatct aactaggtat aatctcaata catatggcat tttttaacat
     3481 atataatctt aaagaaattt atccttaaaa cctccacctt ttacttcttc aacgttattg
     3541 acagaaataa aagcttcact atccatatca taaattaaat ctttaaattt acctatttca
     3601 tttgcagata ttatacaata aatcatcttt ttcttatttt gtgtatatgc accttctgcc
     3661 tccaaaaaag tgactcctct acccatttta ttcattatca ctttggatat ttcatcatat
     3721 ttatttgata ttaaaagtat agattttttt ctatcaaagc aatcttttcc tatatccata
     3781 gtaaaagaaa ttatatacat tgtaattaaa gtgtacatca ccaatttagc tccaaataaa
     3841 aaagctcctg cacatactac aaaaaaatta ataaataaaa atgtattttt cataggtata
     3901 tcatactttc ttttcattat ggctgctatt atatcaagtc ctccagcagt agctcttgct
     3961 ttaaaagtta accccatacc tataccagtt aatgcaccac cataaataca ctgtagcata
     4021 gtatcatcta ttgggaaata tttagcaata tcttgtgtta gccccagtgc tagagaaaat
     4081 agtaacatat tgacaaaact tattagcaaa aatttattat caaaatactt tctagctata
     4141 ataaatattg gtatgttcat caagaaactt gatattccct gatttatacc aaataatttg
     4201 tataatataa cacaaattcc agctactcct ccacttaata acttggctgg tgctagatat
     4261 aaatttattc ctaaggttga tagaatcata cccaatataa tcagacaata ttgtaaaaat
     4321 atattattat caatacttag ctttttactt tcaccataca tcaaaacgca cccctttgca
     4381 taataaataa accaccctgc ataacacacg ataaatttac aaataaattt atcagaagta
     4441 gtttcctaaa aatattattc atcctcactt aaacgtttat tcattaatat tctatcacat
     4501 gacataaata atttatatga tttatataga tttatttatt gtaaattttg gaatattaaa
     4561 acattttatt gaatagataa ttatattaca taaaaaatcc ctatagaaat ctataaacta
     4621 atttctatag ggattttggt taactaacta ttatttttat ttttttataa cttttatacc
     4681 tgtcatatgg tcatttaagt tttgttcctc taaagattta tcatctactc tgtttatttc
     4741 taaagctaaa gtttcagact tgatatattc attaaaatgg tcaacagcct tagcaatttc
     4801 atcatcacca cagtattcta ttactatatt atctaatact tcaaagttat tagattttct
     4861 taattgctgt actttagata taaattctct agcataacct tcattaatca actcttcagt
     4921 tagcttagta tctagtataa caaaaagatt gttttcgaca gatacattaa atccttcctt
     4981 agcagttata cctattaata ctaaatcctt attaaattca aaagcttcac catctaaatc
     5041 aactgttaaa gtttcacctt tttcaagttt attagcagtt tcatgagcat ttaatttact
     5101 taatgcttca acgaataaat tcatcttaga acctaataat ggccctgctt ccttaaagtt
     5161 tggttttaaa ctaaagttca tatactcatc taaatcctta gcaaatatta cctctttaac
     5221 attaagctct tcttttatta aatcaactac atcacttatt gtatcttcaa acttaccatc
     5281 tactaacact ttttgtaatg gttgacgaac ttttattctt tctacttctc ttgaagctct
     5341 acctaaagta actaagtttt tagctaaatc cattttttct tctaatttag tatctactaa
     5401 ctcttcatta catttaggat attcagctaa atgaacagat acttctcctg ttaagtttct
     5461 atacatttct tctgacatat atggtgcaaa tggagctata gctctacaaa gaccatgtaa
     5521 caattcataa gttgtattgt acactgcttt tttatcttct gttaattcag ttgcccagaa
     5581 tcttcttctt gaacgtctta tataccagtt agataaatca tcatttataa aatcttgtat
     5641 cattcttaaa gttttattta actcaaatat agctaaattt tcttctacat ctttcattaa
     5701 attattgaac ttagataata tccatctatc aagttctggt ctatctttat attctacaaa
     5761 aaattcagtt ggatttatat catctgtgtt tgcatataaa gtaaagaagt tatatacatt
     5821 cttcatagta cctataaatt tactttggat ttcttttaat ccatccatat caaatttagt
     5881 tggagtccat ggtggagaaa catatagtaa ataccatctt aatgcatctg ctccatattg
     5941 gtcaaataat tccattggat ttactgtatt tcctcttgat ttactcattt tcttaccatc
     6001 tttatcaagt acaagatctg gaactaaaac actcttatat ggtgcttttc ctaatacaaa
     6061 tgttgatact gcaagtaatg agtagaacca acctctagtc tggtctatac cttctgatat
     6121 atagtcagct gggaacaatt ggtcaaagtt ttccttattt tcaaatggat agtggtgttg
     6181 agcaaatggc atagccccac tatcaaacca acagtctata acctctgtta ctcttgtcat
     6241 tggttttcca catttttcac attttaagtg tatatcatct acatatggtc tatgaagttc
     6301 aactgtttct ggatttacat cttctatagc tttttcagct aattcagctc ttgaaccaac
     6361 tgaatctgta tgaccacatt cacatctcca tataggaagt ggagttcccc aatatctagt
     6421 tcttgatatt gcccagtcat ttagattttc taaccagttt ccaaatctct tctctcctac
     6481 aaaacttggg aaccatttta caccattatt attttctatt aatttatctt taagtttagt
     6541 catctctata taccagcttg gttttgcata gtatactaat ggagtttgac atctccagca
     6601 gtgtggataa ttatgaacta ctttttcttt gctatataat ttattttcag aagctagcca
     6661 ttttattatt tcaacatcta gcccatcttc cattacaaat ctaccttccc aaggagtaga
     6721 tgtaaattta ccagtttcat ctactggttg aataactggt aaatcatatc ttttacctgt
     6781 gttgtagtca tcttcaccaa atgctggtgc tgtatgaact ataccagttc catcttcagt
     6841 agttacataa tctcctactg taataaaaaa tgcttttttg tcaacttcta caaaaggcat
     6901 taattgttca tattctaaac cttctaggtc tttacctttc atttcttcta aaacttcata
     6961 atcttcacct agtactttat cagctaaagg tttagataca taatatacct catcaccttt
     7021 tttaactttt aaataatcta catcagcatt tactgttaat gaaacgtttg atggtaaagt
     7081 ccatggagtt gttgtccatg ctaagaagta ttcatcagca tcttttttct taaatttagc
     7141 tataactgta ttagttttta cttctttata tccttgtgca acctcatgac tagcaagacc
     7201 agttccacat cttgggcaat aaggaagtat tttatgtcct tcatatatat atccttcttt
     7261 gttgaatttg tttagtatcc accatacaga ctctatatag ttattatcta aagttatata
     7321 tggattatct aaatcaactt cataagccat tctctcagtc attattctcc attgtttttc
     7381 aaatgagaaa actgactctc tacatttttg gttaaattca gctattccat attcttctat
     7441 ttgttgctta ctagtaagac ctaactcttt ctctacttgt atttcaactg gtaatccatg
     7501 tgtatcccaa ccagcttttc tctttacttg ataaccagac attgttttat atctacaaac
     7561 tgaatcttta agcgttctag ataaaacatg gtgtactcca ggatttccat tagctgttgg
     7621 aggtccttca tagaatacga aacttgggtc atctttccct ttttctaaag ttttttcaag
     7681 tatatttatg tctttccaat aatcaaaaac ttgtgcttct gcttgtttta cagaactgtc
     7741 taccaatggc ttaaatttcg ccatgtaaat caccctttcc tactaaatat tataattcca
     7801 aaatttttta ttctttcaca taaatttaaa tactaaaaaa ctcgccccct ttaaaccaaa
     7861 gggacgagtt ataaatcgcg ttaccaccct aattctatat gtaaactaaa tactagctac
     7921 atataacact ctaatagtat aacggtacta accggcacag cttagtagaa tttaatcgtt
     7981 cagcctgcag ctcaagagtg atttttatct ttataaattc attggttctc agcataccca
     8041 attctctgta aaattatcta aagataaatg tctctatcaa cgcttttaaa aatttatctg
     8101 tgtttatatg aaattctata acaaataatt tgaatagtca atatattttt gaaaaatata
     8161 ataatttcta tttttcttac atttttatcc tatttttaat ctttgttata gatttctcaa
     8221 actcacacaa ttattctaca taaaaaataa acaaattaca agcttattct aaagttgtag
     8281 cagcttcatc attactataa ttatacactg ttgttccttc aaatgcattt acacattttt
     8341 catctacatc ataaaaaatc tcatcaacac ttcttatttc atcttctaac agtgatttaa
     8401 atttatttct aaatatctta aattctttga ctaaagcatc atactctttt cttatctcta
     8461 taacacggtt attagcttgt tctataattt gtctagcttt taaatcagct tcttcaacaa
     8521 ttatcttagc ctttttatta gccgcactac aagtgtcttc tgctgcactt tgagcagtta
     8581 ttaaagtggc ttttagtgtt tcttcaatat tttcatactt gtttacttgg tcttgatata
     8641 accctaattt ttctttaaga gctgtatttt ccctacatag agattcataa tcttccttga
     8701 tgatatctaa aaattcatca acctcttcat ctctataccc tcttaaacct tttttaaact
     8761 ctttattttc tatctcaatt ggagttagca ttacatttcc tcccagctat aatttaaatt
     8821 agtccttata caattaatct tgcttgaact tttatttttc cttttttagt aatatctcct
     8881 atttgtgtta ccttagctct tcctttgcct ctaatagaaa tcaaatcatc attttttact
     8941 attttagaag ttgaagttat cttttcataa ttgacatgga ctttttctcc atttatgtat
     9001 tttgcactat cttgtctaga tatattgtaa ataccactta tcacacaatc taacctatct
     9061 gaagatactg taaatgatac ttctttatat tttgaagaac tattaacaat attttctctg
     9121 gaaatttcct ttacaattac attatttctt gaaacctttt ctaagtttac aattataaaa
     9181 tcgcaaatat cactacttac aactacttga caaaagctat catgtataat tatatctcca
     9241 attttttctc tttttatacc tagacccagt attgacccta agtaatcttt atgagatata
     9301 tttttaaatt tgaaattccc ttctatttgt aaaaatctaa gagtatcttc tatatcttca
     9361 tattccatgt aaaatggata aataaataca gtgcttcttt cagctcgttc ataacctcca
     9421 tccacactgt attttatttc atttgtagaa tttaatattg ctacagcatt tttcacttca
     9481 tatggattaa gaaactccgt agacttaaca tcatagtttt ttatacaact attagccttg
     9541 tctatcactt taaacatttt atttttcaag tctatatcct ttatgtgatt tgttagtttt
     9601 aatttatcca tcctatcacc ttagaatata accaatacta attgtcttag gaacatcaat
     9661 aatactattg caattaaagg agaaaaatct ataggtccat ttgtatattt atacattata
     9721 gaccttattg gcccttctat tggctctgta agattttcca aaatatcaaa aattttactt
     9781 tgtcttccat ttggaaacca agtcatcaag ctttttataa ctatcatcca tgctattata
     9841 tctaaaagat aataaagtgc aattcttatt gttcccatat attaaccgcc tttttactat
     9901 ttattttgcc aagggaagaa tgctttactt tctaattctt ttttcatatt tgcatctata
     9961 tccacattat taggagctag tataaatatt gatttagaaa ccttttgtat acttccatct
    10021 aagacataaa ctgccccatt cataaagtta aatatagatt tacgaacttc agaattagtc
    10081 aatccttcca aattcactat aactgccctt ctttgtttta gatggtctgc tatagttgca
    10141 gcttcatcat aaaccttagg ttcaactatt acaactttca tttggctagt tgtatgtaaa
    10201 tttacaattt tatttgcttt tgcagtacta aagccactgt tcatttcttc ttcttgaact
    10261 atatcgtcca tgccactttc atattcatct tcaatgtaat cttcttcttc atcaactatc
    10321 caattcttaa atttagatat tattccattt gacattatta acttcctcct taaaatttat
    10381 tcaaatttga gtaattattt tttattgtag tttctttctc caaagattga tgttccaact
    10441 ctaacaatgg tagccccttc ctctatagca atttcataat cgtgactcat tcccattgac
    10501 aaaacatcca tccctacatt agggatgttt agttctttta tttgttttga taaatcttta
    10561 agacctttaa atacacctct tatctcatct tcatcttcta taaaaggtgc cattgtcatt
    10621 agacccttta tttttatatt tttatatttt tctgacactt ctttgacaaa gtctacaact
    10681 tcttctctat caagtccatg cttagactct tctttagata tattcacttg tactaagcaa
    10741 tctatttctc tgtttatttg ttctgcacgt ttttgaattt cttcgcataa tgcaattcta
    10801 tctaaagagt gtatcataca aactttatcg attatgtact taactttatt ggtttgcaaa
    10861 gttcctatca ggtgccagtt taatttgtca cctataatat catactttct agctaattct
    10921 tggggtttat tttcaccaac agaagtgatt ccttccctca ttgcctcatt aactaagtct
    10981 acatcaacag tctttgtgac tgccaaaagg gtaatctctt tgctgtctct atctactttt
    11041 tttgcagctt tatcaatttt gtttcttatc tcagacaaat tatctttaat agaacccatt
    11101 attatcacct actttatcct tatatttgtg tttatactgt taggtcttaa aattattttg
    11161 tcatatagat taacacttgt aactccattt atgtcatttt tataataatc cacaaataca
    11221 gaagattcat tctcataagt tattccttct aatatgacaa attttacact ttgtgattgt
    11281 tcatcaatta catatacacc acgtttattg tcttttgttt ttatagaagt tttaggtatt
    11341 tttaaacatt ccatctgatt atatattata tcaaattctt cgacacttgt atcctgattt
    11401 gcaacaattt ggttacttat ttttaatttt accattattt tattattatt ttcatatatt
    11461 ttgtaaactt ttgcatctaa tttttgctca tgatattcaa ttattacatt ttgatttttt
    11521 tcaaagttat tagaatcttc tttactgatg tatgtagata gataattact ctggtcattg
    11581 gcatcttttt ctatagttcc ttttacatga atctttgcat caactttttc attttttaac
    11641 accaacgtat ctacattttt gctaattatc attactaatg tattaaatac taaatatata
    11701 agaataccaa gtatcactaa tctggtatat tttacttttt tcaaagactc tccccctata
    11761 aaattacttt cattataagt acttcttcta aaaaatgttt attccttttt tataagctaa
    11821 tattttacgt tctaataatt attttttcca tattaaattt attttttatc aatcttccta
    11881 tacaagactg cattatttat atataattgt attataaagt ataagtttaa tttacatttt
    11941 atacgttaag cttactttat atgtttttat agttattttt atttttagat acaatataaa
    12001 accaattaaa atttaaaata acttttgtta tgttgacatt tatttaggta atcatttacg
    12061 aatcaggata acgttatcat aacaaaagtt tatgtcctaa aaaaaataca aggaggattt
    12121 tcttatgtca gaaattttat taggaacagg tttactttta ttggttctat gttgtttcac
    12181 aatttttagt tacaaggccc catatggtat gaaggctatg ggagctctag caaacgctgc
    12241 atgtgctact ttcttagtag aggcattcaa cctttcttta cttggtgatg ttttaggtat
    12301 ggaatttttt agaagtgttg gagctgctaa tggtagtctt ggtggagttg ctgctgctat
    12361 acttgtgcct ttagccttag gtgtatctcc agtttatgca gttttaatag gattatcttg
    12421 tttagatttt tctatattat ctggattcgt tgctggatac tttgtttctt tcttaattaa
    12481 gtttatagag aaaaaagtgc cacctggagt ggatttagtg gttgtaattc ttctatgtgc
    12541 accactatct cgtcttatag catcaaatgt agacccatta gttaatagta cactactaca
    12601 aattggacaa gttttgatag aagctaccaa cattagccct ataataatgg gaattatact
    12661 aggaggtcta ataacagtag ttggaacgtc tccacttagt tcaatggctc ttacatctat
    12721 aattggtctt actggtcttc ctatggcaat aggagcatta ggtgcttttg gttcagcttt
    12781 tatgaactca acactatttg caaaattgaa attaggttct aaaaaagata ttatatctgt
    12841 tgctattgaa ccattaaccc aatcagacat aatttcagct aatccaatac ctatatattc
    12901 tactaatttc tttggaggag ccttttctgg tatcattgta gccttgatgg gtcttgtaaa
    12961 catgacacca ggtacagcta cacctgtggc tggttttgct ataatgtttg cttataatcc
    13021 agttcaacaa gttcttataa cagctggttt atgtgcttta ttcaatgtaa ttgcaggatt
    13081 tttaggttca aaaatattca gtacctttaa agttcttact gctggtgaaa tacgtggtac
    13141 tgatgtagaa gaagaaattg cttaaaaaac aataagattt ataaatttat atctcaagta
    13201 gtagagtttt atattgtagg caattttatt aattgcaaac gttttatttt ttatttatgt
//
LOCUS       DACXMZ010000099        13190 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.100, whole genome shotgun sequence.
ACCESSION   DACXMZ010000099 DACXMZ010000000
VERSION     DACXMZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13190
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.100"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            228..929
                     /locus_tag="JD016_11025"
     CDS             228..929
                     /locus_tag="JD016_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HAU5000167.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDELCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1215..2018
                     /gene="larE"
                     /locus_tag="JD016_11030"
     CDS             1215..2018
                     /gene="larE"
                     /locus_tag="JD016_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HAU5000168.1"
                     /translation="MKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKDTLGEN
                     VIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYHCKKYI
                     FSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEEIRSLS
                     KTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRMHGDIA
                     RIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2073..3221
                     /locus_tag="JD016_11035"
     CDS             2073..3221
                     /locus_tag="JD016_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAU5000169.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3357..4745
                     /locus_tag="JD016_11040"
     CDS             3357..4745
                     /locus_tag="JD016_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAU5000170.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4738..5472
                     /locus_tag="JD016_11045"
     CDS             4738..5472
                     /locus_tag="JD016_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000171.1"
                     /translation="MVNIILNWRFYFMKEKILILEDEIGIRSFVSINLKREGYEIVEA
                     GTGREAIEKMTTEKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDK
                     IEGFISGADDYIIKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKL
                     FKNGKEIELTPTEFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRN
                     KIEDDPSKPKYIQTIWGYGYCFRKEE"
     gene            5475..6908
                     /locus_tag="JD016_11050"
     CDS             5475..6908
                     /locus_tag="JD016_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU5000172.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6930..7667
                     /gene="truA"
                     /locus_tag="JD016_11055"
     CDS             6930..7667
                     /gene="truA"
                     /locus_tag="JD016_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAU5000173.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHSPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    7778..7985
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8238..9437
                     /locus_tag="JD016_11060"
     CDS             8238..9437
                     /locus_tag="JD016_11060"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAU5000174.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9684..10730
                     /locus_tag="JD016_11065"
     CDS             9684..10730
                     /locus_tag="JD016_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HAU5000175.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10838..12001
                     /locus_tag="JD016_11070"
     CDS             10838..12001
                     /locus_tag="JD016_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAU5000176.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     NRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSVVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12090..12470)
                     /locus_tag="JD016_11075"
     CDS             complement(12090..12470)
                     /locus_tag="JD016_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HAU5000177.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(12473..13132)
                     /locus_tag="JD016_11080"
     CDS             complement(12473..13132)
                     /locus_tag="JD016_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAU5000178.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERVSIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         5306 a         1307 c         2280 g         4297 t
ORIGIN      
        1 aaccatgaac atttgttttg tgctatcata tttatataag ggaattgtca taaaaaatat
       61 cttgttcata cagttttata tagaaaaaag aaatgaatct tttctttata taagtatatt
      121 ttgtaaattg cctctatatt tatagtttaa ataatagtgg tattaaaaat ctgacaacaa
      181 aaataaaact ttcaaaaaat taaatctaaa aagaatagga gataaatatg agtattgaag
      241 atttatatga ttttgaatgt gttcctttga acaatgagat tgatattgat gttttatttg
      301 aaaagatgat agaagatgga aaagaaaaag aatatacacc aattatagtc attgatgaaa
      361 aagtagggct attaagaaaa aatatagata tgattactaa agagtatggt tctcttgaaa
      421 actatagaga gtattgttta acaaaatata atgaaattga tgtaaatcaa ttttttgatt
      481 ttaggaagag tgagatttca gaattattaa aggagctaat tgatttagaa gatgattttt
      541 atgattataa acattttaga ccaattatga atatagatat atttcatgaa tataacaaag
      601 tttacatagc taaaattcca acaattaaac catatgaagt atttgcatac atacctattg
      661 gcggatttaa tgattgtcct agagatgaag aacatatagc tattgcaaaa tattggtatg
      721 aaaagcatgg aatctttcca atcgcaattg gatgtgatac agttcaatat tctgtaaaaa
      781 atttaactaa agatggtaaa aaatttgatg aattatgtat tgaattagta ttttattgtg
      841 aggatattat tatacaaggg tatgaaacct taaaggctct taaggatgta ttaaaacgct
      901 ctacaatagt gcttttttgg tgggattgat attccatcat ttagatagta ttaaattgtt
      961 agtcaccaag aatccacttg ctttagttat ggggagtgtg tcaaactagg tttttataca
     1021 cagtgtagca tagtaatatg tttaaattac aaaggttaaa aaaatacttt aatattttta
     1081 atattataat taatagattt aaaggtaatt ataatttata aaataaatac tttatatgtt
     1141 ataatcatta agaatgtaaa aaaataatat atagttttat agttcatatt tttccagata
     1201 tggaggtaaa ttttgtgaag gaaaaagaaa aattagataa attaaaaaaa atgttacttg
     1261 aattaggcag tgttgtagtt gcttattctg gtggagtaga tagtaatttt ttgcttaagg
     1321 tcgcaaaaga tacattaggt gaaaatgtta tagctgtgac tattcatgca atgatgcatt
     1381 caagtaggga aattgaagag gctaaacaat atactcaaaa ttttggtgtt aaacatatca
     1441 tattaaatat agaaaacttt gatttaaaag aatttaaaga aaatggtgtt gatagatgtt
     1501 atcattgtaa aaaatatata ttctctaaaa taaaagaggt agcaaaagaa cataatataa
     1561 agtacatagt agatggaaca aatatagatg atttaggaga ctatagacct gggcttaagg
     1621 cattgagtga attaggagta attagtccat taaaagatag tagtttaaaa aaagaagaaa
     1681 ttaggtcgct ttcaaaaaca ttaggattaa atacttttaa taaaccatca tttgcatgtc
     1741 ttgcgagtag gataccttat ggagttgaaa taacagatga aaatttaaga atagtagaaa
     1801 agagtgaaga atatctatcc aatttaggtt tttctcaatt tagagtgaga atgcatggag
     1861 atatagctag aatagaagtt ggacaggaag aactaggtaa attttttgaa aataacaatt
     1921 ttaataaagt tgatactaaa ttaaaaatat ttggctttaa atatgttaca ctagatatgt
     1981 caggatataa aatgggaagt atgaatttaa atgtgtagat tattttaaaa ataaataagt
     2041 tttatagtta atataaggaa acaggaggtt ttatgaaaaa ttatacgtta atatcgccat
     2101 gtttttttgg aatggaaaaa atgctggcta gagagattac taatttagga tatgaaataa
     2161 taaaaacaga agatggaaga ataacatata aaactgatga gtttggaata gcaaagtcaa
     2221 acatgtggtt gagatgtgct gaaagagtac atcttaaaat tgctgaattt gaggcaaaaa
     2281 gctttgatga attatttgaa aatacaaaaa gaataaactg gtctagatat ataccttatg
     2341 gagctcaatt tcctatatca aaagcttcct ctataaaatc aaaattatac agtacaccag
     2401 atgtacaagc catagtaaaa aaggctatag tagaaagctt aaagaaaagt tatttggagg
     2461 atggtttgct taaagaagat aaggagaaat accctatttt tgtatttata cacaaagata
     2521 aagtaaccat ttctatagat acaacaggag atgctttgca taagagagga tatagagaaa
     2581 aggcaaataa agctccaata agagagactt tagctgctgg gcttatttat ttaactccct
     2641 ggaaagcagg tagagtttta gtagacccta tgtgtggttc tggtactata ttgattgaag
     2701 ctgctatgat tggtataaat atggcacctg gcttaaatag agagtttata tcagaaaagt
     2761 ggagaactct tgataagaaa atatggtggg atgtaagaaa agatgctttt aataagatag
     2821 ataatgaatc taaatttaag atttatggat atgatataga tgaagaatct atagatatag
     2881 ctagagaaaa tgctgaaata gcaggtgtag atgaatatat agagtttaat gtaggagatg
     2941 caactcaatt taaaagtgaa gatgagtttg gatttataat cactaatcct ccttatggag
     3001 aaagattaga agataaagac agtgttaaac agttgtataa ggagcttggt tatgcattta
     3061 gaaaattaaa aaattggtcg tattatttga taacttctta tgaagacttt gaatatgagt
     3121 ttgggcaaaa agcagataaa aaaagaaaat tgtataatgg tatgttaaaa actaactttt
     3181 tccaatatcc gggtcctaag cctcctagaa ataataaata agttttacat gatttatttg
     3241 taaaatataa aattttatta aaataagtaa aacattatga tgccttgaaa cgtctaatta
     3301 ttgtgaggag atttttgtga acaaatttaa agatttatag gattaggagg agttaaatga
     3361 aagtgttaaa gcttattact gtagtagccc tgatgatgtt atttgttact ggatgtaata
     3421 taaaaactga atctccagaa caattagcga agatacctga ctatgatgat acaaaaaaag
     3481 tattgtatga tggtattgac cagttactaa aaccagattc atctatgata ttgccttcta
     3541 atacaaaaga agtaggaaaa ataaataagg tagatttaaa cagcgatgga attgatgaat
     3601 tagtagtatt tgaacaaaaa gaagatttaa gtaataatgt gagtcaggta gggtttgtaa
     3661 ccttgagtta caatggagaa aaatacgtac ttggagacca ttttttagaa aatggcgaat
     3721 caatagaata tgctaatttt tatgatttgg actcagatgg atataaggaa gttatcttat
     3781 tggttaaatc caaagacaaa acgaatatgc atatatataa agtagtagat aatgaaataa
     3841 caaaaatata tgacttggat gcatcatggc ttcaaaataa agaagatttt aatgatatga
     3901 aagtaaaaat tggatatata gatggagatg acaagttaga catattgatg cttcatttga
     3961 ataataagac taatgaaatg tttgcaagtg ttgcaaattt tgatgggaaa atgaaaatca
     4021 aagattctgt taagtttgaa aatgtaaaaa acttaagtga attatatata acattaggta
     4081 atgttgcttc atctgttgga aattcttctg cagctataaa aggtattgta cttgatattc
     4141 ctatattgaa agataataac tatattacgc agatacttta tatgaaagat gggaaaatta
     4201 acaaagcttt tagtgattat gataaaacta ttactaaatc ttattatata cctgtagatg
     4261 atattgacaa agataagatt atagaaatac ctatagttgc aggcagtaca ggtaataata
     4321 aaaatactta tagttctaaa tcatctgcaa ctataagttg gtatagatgg aatggaaaag
     4381 aaggtgctga ctcaagttta atatttacaa gtcagatata ttataattat aaatataatt
     4441 ttaaattatt tattccaaat aatttatttg ataaaatact tgttgaacag gaatttgttg
     4501 gagaaaaagc agtatttaaa ttttactatt ttgactattc agaccgtaag aatttattta
     4561 atatagtagt agagagtaaa aataaacttg aagatagaaa aaatacaggt actcaaaatt
     4621 ctattgtact tcaagagagc gatgaatata ctttcttact agttgtaaac aatgcaaatg
     4681 aaatggataa gctagatatg actatagacg cattaaaaga atatttctca ttaatatatg
     4741 gttaatatta tactcaattg gaggttttat tttatgaagg aaaagatact tatactagaa
     4801 gatgaaattg gtattagaag ttttgttagt ataaacttaa aaagggaagg ctatgaaata
     4861 gttgaggcag gtactggaag agaagctata gaaaaaatga caacagaaaa ggatattaca
     4921 atagctcttt tagatgtaat gcttccagat ataagtggta tagaagtatg taaatttata
     4981 agggagaatt ttgaccaagt aggtataata atgcttactg ctaaagctca agaagatgat
     5041 aaaatagaag gatttatctc aggagcagat gattacatca taaaaccatt tagtataaaa
     5101 gagcttttag tcagagtttc tgctcttctt agaagagtag caaaggatga ttcaagtgtt
     5161 aagtcgagtg agatagtttc tccaccattt atacttgata tagacaaaag aaaattattt
     5221 aaaaatggaa aggaaattga gcttacacca actgaatttt caatagtgaa atatctaata
     5281 tcaaatgcta agcagtcgtt aagtagagac cagatattag acgaagtatg gggaactaac
     5341 tatttatatg attttaaaat agtagatgta aatataagaa ggataagaaa taaaatagaa
     5401 gatgacccat caaaaccaaa atatatacaa actatttggg gttatggata ctgttttaga
     5461 aaggaagaat aatagtgtta cattttgaag gtctgagaaa aaaagtaata aaaaactatt
     5521 ttataataat tattataatg gttactctgt ttgaaggatt gttcatgttt tacatacaaa
     5581 actactatta tgattctgtc aaacagctac ttgaatctga aataaaatat gctgatgaat
     5641 ataatgctat tacaatggaa actacaagtt ttgagaaaaa agtaaaaaat atatttgata
     5701 aacaaccctt aactaaaaat tcagaatttg gaatttcaat aatagataaa gataaaaata
     5761 ttatattaga ccaatatggt tttaaaagta aagaaaaagc aaactatgaa gatgttaaca
     5821 atgcattaaa agatataaaa actaaaaatt taacacctta tacttataga ataccagata
     5881 caggggagca tgttatgagt atctccttac cacttaaggt aaataatata atagaaggtg
     5941 ttgttagata tacagtatca ttagatgcta ttgataatgc aatattaaag caagctacat
     6001 ggcttatatt agcaggaata tttatactta ttatagctat actaataagt cttaaatttg
     6061 ctgagactct tataaaacca ttacgagagc taaagaaatt tgcaaatgaa ttggctgtag
     6121 gaaattataa tataaaatta gaaaagatga agattgtaga tgatgaaatt ggtgatttag
     6181 cacagacatt tgaacacatg gcacatgaga ttgacaaaag tgaaaaatta aaagaggaat
     6241 ttatatcatc tgtatctcat gaactaagga ctcctcttac atctataaag gggtggagtg
     6301 aaaccttagg ttatgaaagt ataacaagag aagaactaga cttaggttta ggcattatac
     6361 aagatgaaac agaaagactt ataaaactag ttgaagaatt attagacttt tcaaggcttt
     6421 cttcagacag gataaaactt catgttgata tagttgatgt tgaaggttta attgtaggtg
     6481 ttgtaaatca acttaaagtt aaagcagctg aaaaagatat atcactttta tttgaatttg
     6541 aaaatgaatt tatagagaac attcaaggag ataaaaatag attaagacag gttctcataa
     6601 atttaataca aaattcattt aaatttacga gtcaaggtgg ttacataaaa gttgtagctt
     6661 ctcaagatga agagataaca accatatcag tagaggataa tggttcaggt atagaaaagc
     6721 aaaatttaaa taaagtgtta gataaatttt tccaagaaga ttataataag gctggtagtg
     6781 gattaggact agcaattagt aatgaaattg taaaacttca tggtggaaga atgaaaatag
     6841 aaagtgaaaa gaatgttgga acaaaaataa cttttaatat aaaaaataaa tttgctaaac
     6901 aagcataaat tcttagaaag aggattaaga tgagaaacat aaaaatgata gtagcatacg
     6961 atggttcaag atataaagga tatcaaaaat taggtgacaa taatatgact attcaagaaa
     7021 aacttgaaaa tgttttaagt aaaatgacta atgaaacagt ggagataata ggttctggaa
     7081 gaactgatat gggagctcat gcaagaggac aagtggtaaa ttttagaact aattgtatgg
     7141 attctttgga taaaattcaa aaatatttat atgaatattt accagaagat atagttgtaa
     7201 aaactgttga agaagttgat gaaagatttc attcaagata caatgtaaaa tcaaagactt
     7261 atatgtataa aattgacaat aataagtatc atagtccttt tataagaaaa tatgctactc
     7321 atgtaagtaa aaaattagat ttagatagaa tgagaaaagc aagtgagtat ttagtaggag
     7381 aacatgattt taccagtttt gcttcttcta aatctaagaa aaaatcaaat gtaagagaaa
     7441 tttattctat aaatataaaa gaagatgata atgttataga aatatatgta gaaggaaatg
     7501 gatttttgta taacatggta aggataatag taggtgcttt aatagatgtt ggtctaaaaa
     7561 gaagagctcc acaagatatt aagtatatgc ttgaatcaaa agatagatgt caatcatcag
     7621 atactgcacc agctaaagga ctatgtcttt ggaaagtaac atattaatca attatataaa
     7681 actctcttta aaataaaaat aaaataaatt atatagttga cataaatgga aaaataaact
     7741 attatatatt ttataagaga gatataaaac tttatattta aatcctgtga gaaagagagt
     7801 aacttctatg tgttttatag agagctgagg atggtggaag cttggcaaat aagtattagt
     7861 gaaaagaact ttggaggata ctaattgaat ttagtatgtt agtaggtaat ctcgccttaa
     7921 gagaaagagt ggataagttt atcttatcaa ttagagtggt aacgcggata tatttcgtct
     7981 cttggttttt ccaagaggcg ttttttaata tatttaatta ttagtgttta tctggtatgt
     8041 tgaacattta ctaattgatt agctaagttt atcaataaga gtggtaccgc ggatatttcg
     8101 cctcttagta ttttaaaata ctaaggggtt tttttagtat taaaatgatt ttatgtgagt
     8161 ctaaattctc agagtcattc aatattaaga atttctataa aataaaaatt ataatttaag
     8221 attgggagga attaaaaatg aaggaaaaag tagtattagc atattcagga ggacttgaca
     8281 catcaatcat aataccttgg ttaaaggaaa attatgaaga tatagatgtt atagctgttt
     8341 gtggaaatgt aggtcaggaa gataaaatgg aagatgtata tgaaaaagct ttacaaagtg
     8401 gagcatcaaa ggcatatgtg gatgatataa gtgaagaatt tgtaactgaa actatattta
     8461 aagccgtaaa agcagaggct aaatatgaag gaaaatactt attaggaact tctttagcaa
     8521 gacctataat agctaaaaaa ttagttgaag ttgctcataa agaaggtgca aaatatatat
     8581 gccatggatg tacaggaaaa ggtaatgacc aagttagatt tgaagctaca attgcagctc
     8641 ttgacccgac tataaaagtt attgcacctt ggagaatttg ggatataaaa tcaagagaag
     8701 atgctataga ctatgctgaa aaacataata taaaggtaac agcaactaaa gctaaaatat
     8761 attcagtaga tgctaacctt tggcatgttt caacagaagg tggagatata gagcacctag
     8821 aaaatgaaca taaaaaagat gtttataaac aatgtgtaga cccagaagat gcatgtgatg
     8881 tagctgaata tgtagaagtt tactttgaaa aaggtgtacc taaaaaggta aatggtgaag
     8941 aattatctcc agttgcactg attcataagt taaatgagtt gggttgtaaa catggaattg
     9001 gtgttataga tatagttgag aatagacttg ttggtatgaa atcaagaggt atatatgaga
     9061 ctccaggagg aacaatcctt tatgaagcac ataatatttt agaaagtgct actttagaca
     9121 aagatacatt acatttcaaa caaatggtat cttataaata cggagaactt atttacaatg
     9181 gactatggta ttgtaaatta agagaatcta tagatgcatt tatggaacaa actcaagata
     9241 atgttacagg aacagtaaaa gttaaacttt acaaaggaaa tataaaacct gctggaatat
     9301 ttactgaaaa tgctctttat gatgaaggaa tatcttcttt tggaaatagt gaactttatg
     9361 accacaaaga tgcagaagga tttattaatt tattcacttt accattaaaa atcagagcta
     9421 tgaaagctgg aaaataataa aataaattat ttaagagtgt ataaaaacta tttttaggtt
     9481 tttatatgct ctttttttat ttgaatttat taacttatac agtaaatagt ataaaaaaat
     9541 atattatagt tccaaatggc ttaataatgt gaaaatataa gacctaaaat ataaatttta
     9601 taaatacaat agttattgac tctctcattg taagagggtt tatactgaat ctaatgaaaa
     9661 tttatagtga ggagatagat tatatgagaa aaataattga aattaaacat cattcacatg
     9721 attatgagtg tatgtggaat ggtatagaag atttatatat aaataaaacc ggtgaatgcc
     9781 ttccaaacga ttttttcttt ttactgtcag gttttggttc attttgttat atgaaaacaa
     9841 ataaagctga tttgaaaaga atggtagctt tgggagatgg aaggacaaaa cagatgtata
     9901 aatttttagc accaattgtt ggatttgaat acaaacatca tgagtttaaa aagtatgagc
     9961 aagcattaaa gaaagcaaaa tctgaaattg atgatggata tccagttatt ttaggagctt
    10021 tagatatgta ttatctacca tattataaga agctatatca taaagaacat attccttttc
    10081 attatatatt aatggtagga tatgatgatg aaggaatata tctgtatgat tgtggtagaa
    10141 cagaattatt acatctttct tatgataaat tacgtgaaag catgaattgc aattcccctg
    10201 gattaagcaa agaaaatact atttgcacaa taagaatgaa tacttcaaag aataaatata
    10261 acattgctag agaagctatt gctataaagc gtgatatgtt tttaaatcca cctaaaggat
    10321 ttttgggata taaaggtttt gaaaagttta ttaaagagtt gccacagtgg aacactgaat
    10381 tggataaaga tgattatgat aagattttga ccaatatggt tatgtttttt ggaacagtac
    10441 caacaattcc aaatgcaatc aaaggaatta aagaaccaga tactgttcaa tttaaaggtg
    10501 gatttgataa aatgagtaga atgctcaaca gtattgggga ggaatacaaa aacgactctt
    10561 ttatacaagt agctgatatt tttgataaaa ctgctattat aattgagaaa atatccaata
    10621 ttataataga ttatttaact caaacatgtg atgatacaga acaacttccg ttattgttct
    10681 ctgaggtctt agagcatatg aaaacaggat atttaatctt ggatgtataa aaaatatatt
    10741 gttgacccta acattacgta atagtgtaat atgaaaatat agcttgaata tattagtagg
    10801 tattgatggc tgtaaaatta aaatttgagg tgtggatatg agaacagtga aacaagtttc
    10861 tgatttaact ggaataagtg tgagggcatt acattattat gatgaaatag gtcttttaaa
    10921 accaaataaa attacagatg caggatatag actttatgat gatgaatcta ttaaaactct
    10981 tcaacaaatt ttatttttta aggagattga tattccttta agagaggtta aggaaataat
    11041 gtcaagccaa tattttgata aggttgaagc tctaaaaaat caaaaaaaat tacttatttt
    11101 aaaacgaaag agattggatg aattgataga gcttataaat caaactctaa gaggagaagg
    11161 aaatattgat tttaaagagt ttgatatgag cgagtatttt gatgttttag aagaatttaa
    11221 aagagagcat aggaataagg taattaaaat ttatggtagt gtagaaaaat acaatgaata
    11281 tatagaaaga gttaagtcta atgaagagaa aattgctaaa atggctataa aacaatatgg
    11341 aactattgag aagtttgcta aagctataaa aacaaatttt agcagtgata tattgaattt
    11401 aggagaaaaa tttgatagat ataaaaatga ttgtttaaaa gaaaaacatc caaaattaaa
    11461 agaattatat agaaagcttg tagaagattt aaataggaat ccttcttcaa cagaccttca
    11521 agaaatagca aaagagataa cagatatatc taaaaaagat tatgaaattt tctctatgga
    11581 tactggtgat gacaactggt attatatggt tcaaaattat ctagtaaatc ctatgtggat
    11641 agaagaagta gataaaaagt atggaagtgg agcaggaaag tttattggac aagtactaaa
    11701 gacttatcta agagatagaa aaccaaagat aaatacattg tatgaaaaac ttgtagaaga
    11761 tttgagtaga gattgttctt caagggaagt tcaaagcgtt gttgaagaaa tagacaatga
    11821 aatgaaaaga agtaatgaat tttataaaat agacaatggt gaaagatact ttgattatat
    11881 gtcagagctt tatttacaag attctaacta tataaaagta actgataaga attatggtga
    11941 tggtgcatct aagtttattg gagaagcttt taagatttat tttgataata ataattgttg
    12001 aaaatttgga agtaaaaaat atgtaagttt tataaaagca caaaaataag gatgtagatt
    12061 agctccatct atatccttat ttaaagttat taaaatatat agtttacatc aaacttaaca
    12121 ctttcaataa tatttcccaa atatgttttt atactttcaa cttcttctaa agattttaat
    12181 ttattttcag tataaattgc tttgtccaga atattaaacc ttaatcctat ttcatcaaag
    12241 tactcatgat ttaaaacttt gtaccaatgg tttttttctt tattccaact cgtagatatt
    12301 gaatcagctt ctttttgagc tttttcccaa tcttcatttt ctatagattt ttctaaaaca
    12361 gatatattgt ctttatatcc ttctgtaaaa tcatatagtt tattgttaac ataaaatcca
    12421 aataatacaa ataagatagt ccatagtata acaaatgttg tagatttcaa tattaatcac
    12481 cctttttatt ttgaataaat attttatcat tttcatctaa aacacaaata agaacatctt
    12541 taaaagtttc tatgtgatta gattttaaaa tacccattaa ccagttagta tctttttgag
    12601 ctatgtctag atttttattt attattctac catccagtat aagtgggata ggtaaatgat
    12661 taaaagctct tggaggagta ctattgtagc ttgatgctgg tacaacactt agattaccat
    12721 cagtctccaa aatagcatat tgtacatctt ttaggttaaa gtatccttga atcctaagtt
    12781 gctctaataa ttcatctata ctaactcttt cttttttaag ctctttatat acaatttttc
    12841 ccttatctat taatacagaa ggcttacctg ataaaaatga acttaatttt ctgcttttta
    12901 atgataagaa tgaaataaga gtctgcataa ttacaagtcc agatattgct gcaacaccat
    12961 ttatgattgg tatattatta ttttccattg ggacagatgc aacttctgca attaatagtg
    13021 ttataactaa atcaaaacag ttcatttcag ctatttcacc ttttcccatg actctaagtg
    13081 caattagcac tgttatgtat aatataatac ttcttattaa tactactgtc atatctatca
    13141 attactcctt taaaaaatat ttacacttag ttagtatatc ctatataaaa
//
LOCUS       DACXMZ010000100        13117 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.101, whole genome shotgun sequence.
ACCESSION   DACXMZ010000100 DACXMZ010000000
VERSION     DACXMZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.101"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            409..1608
                     /locus_tag="JD016_11085"
     CDS             409..1608
                     /locus_tag="JD016_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HAU5000179.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            1647..2447
                     /gene="hadI"
                     /locus_tag="JD016_11090"
     CDS             1647..2447
                     /gene="hadI"
                     /locus_tag="JD016_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HAU5000180.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            2462..3688
                     /locus_tag="JD016_11095"
     CDS             2462..3688
                     /locus_tag="JD016_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HAU5000181.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            3688..4815
                     /gene="hadC"
                     /locus_tag="JD016_11100"
     CDS             3688..4815
                     /gene="hadC"
                     /locus_tag="JD016_11100"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HAU5000182.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            4863..5996
                     /locus_tag="JD016_11105"
     CDS             4863..5996
                     /locus_tag="JD016_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAU5000183.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            6029..6814
                     /locus_tag="JD016_11110"
     CDS             6029..6814
                     /locus_tag="JD016_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAU5000184.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            6833..7870
                     /locus_tag="JD016_11115"
     CDS             6833..7870
                     /locus_tag="JD016_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAU5000185.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            8353..9984
                     /locus_tag="JD016_11120"
     CDS             8353..9984
                     /locus_tag="JD016_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HAU5000186.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(10119..11045)
                     /gene="fba"
                     /locus_tag="JD016_11125"
     CDS             complement(10119..11045)
                     /gene="fba"
                     /locus_tag="JD016_11125"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HAU5000187.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            11375..11704
                     /locus_tag="JD016_11130"
     CDS             11375..11704
                     /locus_tag="JD016_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 family protein"
                     /protein_id="HAU5000188.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYLEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            11704..12426
                     /locus_tag="JD016_11135"
     CDS             11704..12426
                     /locus_tag="JD016_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAU5000189.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            12459..>13117
                     /locus_tag="JD016_11140"
     CDS             12459..>13117
                     /locus_tag="JD016_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAU5000190.1"
                     /translation="MKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRAGV
                     KKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMDLF
                     LKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVEEC
                     MLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTSSQ
                     E"
BASE COUNT         5233 a         1554 c         2504 g         3826 t
ORIGIN      
        1 aattacaatg ataacatgtg tttttgagtt tagcaatgaa aacttttttt gaaatgtcaa
       61 gctttttcat aaaaaaatat ataaaaaaaa cttttataag catagaaaga atagcaaaat
      121 gcaatggata gtggcaagat gccacgatat gaaaaagatt attcaattgc atattgttaa
      181 aaaacattca aatcaagcga tattttgatt aaatataaag ttggcacgat aaatgcttaa
      241 caatataggt gtaagacaat aaaacaataa aaaataattt taataatttt taagttattt
      301 tagataagat taggaacttt gacatcagac agttaatatc agaaaaaagt attttatgag
      361 tgatttaatc gaaattttat aattgatact attaaggagg aaaacgttat gcttttagaa
      421 ggagttaaag tagtagaact ttcaagtttc atcgcagcac catgttgtgc aaaaatgtta
      481 ggtgactggg gtgcagaggt tattaagatt gaacctatag aaggtgatgg aataagagtt
      541 atgggtggaa catttaaatc tccagcatca gatgatgaaa accctatgtt tgaattagaa
      601 aatggaaata aaaagggtgt aagtattaat gtaaaatcaa aagaaggagt agaaatatta
      661 cataaattat tatcagaagc agacatattt gtaactaatg ttagagttca agcattagaa
      721 aaaatgggta tagcttatga ccaaataaaa gataagtatc caggattaat attctctcaa
      781 atattaggat atggtgaaaa aggaccttta aaagataaac caggatttga ctatactgca
      841 tacttcgcaa gaggaggagt tagccaatct gttatggaaa aaggaacatc tccagcaaat
      901 acagcagcag gatttggtga ccactatgca ggtctagcac tagcagcagg aagtttagca
      961 gcattacata aaaaagctca aactggtaaa ggtgagagag taacagtaag tcttttccat
     1021 acagctatat atggaatggg aacaatgata acaacagcac aatacggaaa tgaaatgcct
     1081 ttatcaagag aaaatccaaa cagcccatta atgactacat ataaatgtaa agatggaaga
     1141 tggattcaat tagctttaat acaatacaac aagtggttag gcaaattctg taaggttata
     1201 aatagagaat atatattaga agatgataga tataataaca tagattcaat ggttaatcat
     1261 gttgaagatt tagttaagat agttggagaa gctatgttag aaaaaacatt agacgagtgg
     1321 tcagctttat tagaagaagc agacttacca tttgaaaaaa ttcaaagctg tgaagattta
     1381 ttagatgacg aacaagcttg ggcaaatgac ttcttattta agaaaacata cgatagcgga
     1441 aatacaggtg tcttagttaa tactccagtt atgtttagaa atgaaggaat taaagaatat
     1501 acaccagcac caaaagtagg tcaacatact gtagaagtat taaaatcttt aggctacgat
     1561 gaagagaaaa taaataactt taaagatagt aaagttgtaa gatattaatt agtaattcta
     1621 gattttttaa aaggagaatg ctaaagatgt acacaatggg attagatata ggttcaactg
     1681 catcaaaggg agtaatctta aagaatgggg aagatattgt agcttctgaa acaatatcct
     1741 ctggtactgg gactactgga ccatcaagag ttttagaaaa attatatggc aagacaggtc
     1801 ttgcaagaga agatattaaa aaagttgtag ttacaggata tggaagaatg aactattcag
     1861 atgctgataa gcaaataagt gaattaagct gtcatgctag aggggtaaat ttcataattc
     1921 cagagacaag aaccattatt gacataggtg gtcaagatgc aaaggtatta aaattagata
     1981 ataatggaag actattaaac tttcttatga atgacaaatg tgctgcaggt acaggaagat
     2041 ttttagatgt aatggcaaaa ataatagagg ttgatgtatc tgaactcgga agtatatcta
     2101 tgaattctca aaatgaagta tcaataagca gtacatgtac agtatttgca gagtctgagg
     2161 ttatatcaca tttatctgaa aatgcaaaaa ttgaagatat agtggcaggt attcatactt
     2221 cagtagcaaa gagagtttct agcctagtaa aaagaatagg agtacaaaga aatgtagtta
     2281 tggttggtgg agttgctaga aatagtggta ttgtaagagc tatggcaaga gaaatcaaca
     2341 cagaaattat tgtacctgat atacctcaat taactggtgc tttaggagca gcgttatatg
     2401 cttttgatga agcaaaagaa tcacaaaaag aagtgaaaaa tatataaaaa gagggatgaa
     2461 aatgtctgaa aaaaaagaag ctagagtagt aattaatgat ttattagctg aacaatatgc
     2521 aaatgcattt aaagctaaag aagaaggaag acctgtaggt tggtcaacat cagtatttcc
     2581 tcaagagtta gcagaagtat ttgacttaaa cgtattatat ccagaaaacc aagcagctgg
     2641 agtagcagct aaaaaaggtt ctttagaatt atgtgaaata gctgaatcta aaggatattc
     2701 tattgaccta tgtgcatatg caagaacaaa ttttggtctt ttagaaaatg gtggatgtga
     2761 agctttggat atgccggctc cagatttcct actttgctgt aacaatatat gtaaccaagt
     2821 tataaaatgg tatgaaaata tttcaagaga attagatata cctttaataa tgattgatac
     2881 aactttcaat aatgaagacg aagttactca atcaagaata gattatatta aagctcaatt
     2941 tgaagaagct ataaaacaac tagaaattat atcaggaaag aaatttgacc ctaagaagtt
     3001 tgaagaagta atgaaaatat cagctgaaaa cggaagacta tggaagtatt ctatgagttt
     3061 accagcagat tcttctcctt ctccaatgaa tggatttgac ttatttactt acatggctgt
     3121 aatagtttgt gctagaggta aaaaagaaac tacagaagca tttaagttac ttatagaaga
     3181 attagaggac aacatgaaaa ctggtaaatc ttctttcaga ggggaagaaa aatacagaat
     3241 aatgatggaa ggtatacctt gttggccata tataggatac aagatgaaaa cattagctaa
     3301 atttggagtt aacatgacag gtagtgttta cccacatgct tgggcattac aatatgaagt
     3361 taatgattta gatggaatgg cagtagcata tagtactatg tttaacaatg taaacctaga
     3421 ccgtatgaca aaatatagag ttgattcttt agtagagggt aaatgtgatg gagcattcta
     3481 tcatatgaac agaagctgta aacttatgag tttaatacaa tatgaaatgc aaagaagagc
     3541 agctgaagaa actggattac catatgctgg atttgatggt gaccaagcag accctagagc
     3601 tttcactaat gctcaatttg aaacaagaat tcaaggttta gttgaagtaa tggaagaaag
     3661 aaaaaaactt aatagaggtg agatataatg gaagctattt tatctaaaat gaaagaagta
     3721 gttgaaaatc caaatgcggc tgtaaaaaaa tataaaagtg aaactggtaa aaaagctata
     3781 ggttgtttcc cagtttattg cccagaagaa attatacatg cagctggaat gcttccagtt
     3841 ggtatatggg gaggacaaac agaattagat ttagctaaac aatatttccc tgcatttgca
     3901 tgttcaataa tgcaatcatg tttagaatat ggattaaaag gtgcttatga tgaattatct
     3961 ggagttatta taccgggtat gtgtgataca ctaatttgtt taggacaaaa ctggaaatca
     4021 gcagtacctc atataaaata tatatcatta gtacacccac aaaatagaaa acttgaagct
     4081 ggtgtaaaat acttaatcag tgagtacaaa ggcgtaaaaa gagaacttga agaaatttgt
     4141 ggatatgaaa tagaagaagc aaaaattcat gaaagtatag aagtttacaa tgaacataga
     4201 aaaactatga gagactttgt tgaagtagct tataaacatt ctaatactat aaaaccatca
     4261 ataagaagct tagtaattaa gagtgggttc tttatgagaa aagaagaaca tactgagcta
     4321 gtgaaagatt taatagcaaa attaaatgct atgccagaag aagtctgttc tggaaagaaa
     4381 gttttattaa caggtatatt agctgattct aaagatatat tagacatttt agaagacaac
     4441 aatatatcag ttgtagctga cgacttagca caagaaacaa gacaattcag aacagatgta
     4501 ccagcaggtg atgatgcgtt agagagatta gcaagacaat ggtcaaacat agaaggatgt
     4561 tcattagctt atgaccctaa gaaaaaacgt gggtcactta tagtagatga agttaaaaag
     4621 aaagatatag atggtgttat cttctgtatg atgaaattct gtgacccaga agaatacgat
     4681 tatcctttag ttagaaaaga tatagaagat agtggaatac ctactttata tgttgaaatc
     4741 gaccaacaaa ctcagaataa tgaacaagcc agaactcgta ttcaaacttt tgctgagatg
     4801 atgagtttag cgtaagttca ttaaaataaa tttactataa aatatgaaaa ttggaggaag
     4861 ctatgttata taacaaagaa caagaacttt taagaaaagc agtaagagat tttgttagta
     4921 aagaattaga tactttacca gcagaaatgg ataaaacagg tgtaatgcct aaggaattaa
     4981 tcaaaaaatt agctgatgca aaatttataa gcagtaatat cccagaagaa tatggtggtg
     5041 ggggagcagg atatgtttct tacgctatag taatggaaga aatagctaga agatgtgctt
     5101 caacagctac ttttgttaca gctggttctt ctcttgcttc attaccaata ctttacaatg
     5161 gtactgaaga gcaaaaacaa aaatacttaa aaggtatagc aacaggtgaa ttaataggag
     5221 catttggttt aactgagcca ggagctggtt ctgatgcagg tggacaacaa acaacagcag
     5281 aattagttgg agaccactat atattaaatg gtagaaaaac tttcataact aatggaccat
     5341 tctgtgatgt agctatagta atagcagtta cagatagaag taaaggactt agaggaacat
     5401 cagcgtttat agtagaaagt aaatgggatg gtttctcaac aggagctcat gaagataaga
     5461 tgggtataag aggaactgaa acttctgact taatatttga aaacgttaaa gttccaaaag
     5521 aaaacttact tggaaaagaa ggtcaaggat ttaagatagc aatgggtact ctagaagttg
     5581 gtagaatagg tgttgctgcc ttagctctag gaatagctca aggtgcttta gatgaagctg
     5641 ttaaatatac aaaacaaaga gttcaatttg gtaagcctat agctaaattc caaaatactc
     5701 aatttactat agctgacatg gaaacaaaag tttgtgcagc tagaggatta gtttatgatg
     5761 cagcacaaaa gagagatgca ggaatgagag ttgctcaaga atctgctatg gctaaatact
     5821 atgcatcaga aattgcaaat gaagttgctt ataaagcatt acaacttcac ggtggatatg
     5881 gatttataaa agattatgaa atcgaaagaa tgtacagaga tgctagaatc gtatcaatat
     5941 acgaaggaac atcagaagtt caaaaaatgg taatttcatc aaacgtatta aaataataaa
     6001 taatctaatg aaattggagg tatagacttt gaaaatatta gtttgcgtaa aacaagttcc
     6061 agatactaac gaagtaagaa taaataaaga aacaggtact cttataagag atggagttcc
     6121 aagtatatta aacccagatg atgcaaatgc attagaggaa gctttaaaaa ttaaagatga
     6181 gcatgatgat gtaactataa cagttataac aatgggacct cctcaagcag actttatgct
     6241 aagagagtgt ttagctatgg gtgctgatga tgcaatatta ttaagtgata gagcatttgg
     6301 aggagctgat acttgggcta catcaaatac tattgcagcg ggaatatcta aagtaggtga
     6361 ctatgacata atatttgcag gaagacaagc aatagatgga gatactgcac aagttggacc
     6421 acaaattgca gagaaattag acattcctca agttacatat gtacaagatt tcaaaataga
     6481 aggaaaagat ataatagttc aaagacaatt agaagatgga tacgaattaa taaaagtaag
     6541 tacacctgta cttttaacag cagtaaaaga gttaaataca cctagataca tgtctgttga
     6601 taaaatagta aaagcatata aacatgatgt aaaagtttgg acaattgatg atttagatgt
     6661 aaataaagaa gaagttggtt taaaagcatc accaactaaa gtatttagat catttactcc
     6721 tgaaccaaga ggaaaaggag aaatcttaga aggtaaagct gatgaaatag ctagtaaaat
     6781 aataatcggt ctaaaacaaa agcacattat ataagttgga ggagtacaaa ctatgaatga
     6841 tataaaagat ttaagttctt ataaaaacgt atggatattt gcagaacaaa gagaaggaaa
     6901 aatagctcca gtagttatag aattattagg agaaggaaga aaattagcta aagaagtaga
     6961 tgcagaactt tgtgcaatat tattaggaaa agatgttgat ggattagcta aagaattaat
     7021 cacttttgga gctgacaaag tttatgttgc agatgatgct cttttagaaa aatatacaac
     7081 tgatgcatat acaaaagtaa taaaagatgc aatagatgaa ataaaaccag aaataatgct
     7141 tttcggagca actcatatag gtagagactt agcacctaga atagcttcaa gagttggaac
     7201 tggattaaca gctgactgta ctaagttaga aatagaccca gaagataaga aaataaaaca
     7261 aactcgtcca gcatttggtg gaaacataat ggctacaatc atttgtccaa accatagacc
     7321 tcaaatgtct acagttagac ctggtgttat ggataaagct gaaaaagacg aaacaagaac
     7381 tggtgaagtt atagcattag actacaaaat aactcaagat gatataagaa ctactgtttt
     7441 agaaacagtt aaaactaaga aagatttagt atctcttaca gatgcaaatg ttatagtatc
     7501 aggtggttta ggattaggtg gaccagaagg atttgaaatg cttaagaaat tagctgacaa
     7561 attaggtgga gtagttggtt cttctcgtgc ggctgttgat gctggatgga tagaccattc
     7621 tcaccaagta ggtcaaacag gaactactgt taaaccaaac ctatatatag cttgtggtat
     7681 atcaggagca atacaacatt tagcaggtat gcaatcatca gatttcataa ttgctataaa
     7741 caaaaaccca gcagctccaa ttttagaaat cgctgactat ggagtagttg gagacttaca
     7801 tgaaatagtt ccaatgctta tagaaaaatt agatagcgtt gatgatttat tagaagctat
     7861 aaaagcttaa tagatacaaa ctatataaat attactgatt gaataagtct tattagttag
     7921 taacataatt attaaccctt tgataagtta ttgctagtgt gtatttgtca gttattaaaa
     7981 agtctgtttg aataaaattt tataatacaa gataatctgg ataatcctta tatgtatatt
     8041 gcttacaaat tgtgagttat attctgtaag gattatcttt gtttttttaa ataaatctta
     8101 aatttaatta aattgtatta aatagacaaa taattactaa atatgtgata aaatacaatt
     8161 aaaagcaaaa tgaaattttt aacaaactat aatcattaaa attatatctt agttacgttt
     8221 taatgaaata aactacttga aaagacgaat attataagga gttgattata tgtgctgatg
     8281 gtatttattg taagcaaaca ataaattttg tatgtagttc aataaattac ttaatgaaag
     8341 gtgcgaatat taatggataa taatttaata gggcttatag aacatgtaga aaatccagcc
     8401 attttatgta aagaatcagg cgaaataata tactgtaatc atctaataga tagtattttt
     8461 agttttctag atataaaaaa gcctagaaat ataaatgaat tagattcaaa ttttgacaaa
     8521 acagaaatac tgacagatag taaaaagaaa atagctttca gagagttaag gatgactgca
     8581 catatataca atatgaaaga taataataat gaaaataata tagtttattt atttgagaag
     8641 tctctaatat cggataaagt aattgaagat ataatagagc atatagatga agttgttgtt
     8701 gtgtttaaca aagatggggt aattgaaaag atgaacactg tcagtgatga gatattacct
     8761 tttaaaagaa ctgaggtact tggtagaaat ataacagacc ttgtgagaca aggattagtt
     8821 gaagaaccaa taatattaaa catgcttaaa gtgaagaaaa agatttatag aaatatcgtt
     8881 tacccagatg ggaagcttat tgcatatacg gcagttccaa gatgggattc taagggaaag
     8941 cttacaggag gagtactaac agggagagat atttcaaggg ttattaagct tgaatctcag
     9001 ataaaatata gtgatatatc agaagacaca gagtatataa gtcaaagtaa gattatggac
     9061 aatataaaaa aagttgttaa gagagctgca gcctcagatt cttctatatt tataaatgga
     9121 gaatcaggtg tgggaaaaga aataatagca agaacaatat ataagtatag ttcaaggaga
     9181 gacaaacctt ttatagctat aaactgtgga gctataccaa atgaattatt agagtcagag
     9241 ttttttggat atgaagaagg ttcttttact ggagctaaga aaaaaggtaa aaagggactt
     9301 tttgaagagg cgaatggtgg aactattttc ttggatgaga taggtgagtt accaatgcag
     9361 atgcaaaaaa aattacttag ggttatacaa gaaaatacta ttactagaat aggtggtagt
     9421 aagcctataa aaatagatgt tagatatata agtgctacta atatttctca tgaagacctt
     9481 agaaataacc ttaaatttag acaagatttg tactatagat taagtgttat tccagtaaaa
     9541 atacctcctc ttagagaaag aaaagaggat atagtaccat tagtaaatta tttcttaaaa
     9601 ctttacaatg aaaagtacaa tagagaagta gaagtttcgc ctaaagttat tgagttatta
     9661 gaagaatatt cttggccagg aaacataaga gaattaaaaa acatcattga aagatttgta
     9721 gtattatcag ctaaaaatgt aattggagaa gatgaattta acatgcttat aaatttagat
     9781 atgattgata atgaaacaga tgatttatca ccaatagtgg taaatggaat tatgaatctt
     9841 aatgatgcat ataaaatagt ggaccaaata atgataagta aggctataaa taagtatggt
     9901 tcaataacaa aggctgctga agttataggt atatatcctt ctaccatcca tagaaagata
     9961 aagagtggac atattcacgt ttagctgtca gtttaaaaag acaatataat taaaatatta
    10021 taagatacat aattgataca acttaaaaga gatggcttaa aaataaattt aattacttta
    10081 agtcatctct tttcttttat aaatatttaa aagttatatt agtttataga gttagcagaa
    10141 cctaaaacat catctatctt gctttcaact acagcttgta tagcatctct accagcacca
    10201 atgtattttc ttgggtcaaa ttcttttgga ttttcagcta agaattttct gatagcagca
    10261 gtcatagcta gtcttaagtc agtatccatg tttattttac aaacagccat agaagatgct
    10321 tttcttaaca tatctacagg tacaccttta gcaccagcta tatttccacc aaattcatta
    10381 catgtagcaa cagcgttttg gtcaacagca gatgcaccat gaagaactat tgggaaacct
    10441 ggtaatttgc tttggatttc ttctagtata tcgaatctta atttagcttc tcctttgaat
    10501 ttgaaagcac catgagaagt tccaatagct attgctaatg aatcaacacc tgttctttca
    10561 acgaactcaa ctgcctcagc tggttgagta tacttgtgaa catcagaagt aacatcatct
    10621 tctgttccag ctagaacacc aagttctgct tcaacaacaa cacctttaga gtgtgcatat
    10681 tcaacagctt ctttagttat tctaacattt tcttcaaagt caaaatgaga accatctatc
    10741 ataactgaag agaaaccagc atcaatacaa gttttaatag catccatatt tggaccatgg
    10801 tctaagtgaa gagctacatc tacacctatt tcatcagaag cagccttaac catttcaact
    10861 aatgtatgag gtccagcata tttaacagct gacatagaag cttgtatcat aacataagaa
    10921 tttttagctt tagcagcttt taaaacacct tgtaattgtt ctaagtcact tatgttaaat
    10981 gcacctatag caaatccacc ttcgtaagct tttttaaaca tttcttttgt agtaattaat
    11041 gccattttaa actcctccta ttaactttta aaatttaccc atcacttata ttataacgtt
    11101 aaatcaaaaa aagtatatag taaattgtaa taactataaa ttacaagtat aatgttaaat
    11161 agaagacaat aggattaaat aaaataataa agaatataaa caatttagga aaattagcta
    11221 tataatgcta actataaggg taggaactac cttattagtt tggagatact gataacaata
    11281 gttgtcttga ccaagaagtt accacttcaa acgttatcga agggaattaa gtggatgata
    11341 attcactaaa aatttaaaat aaaggagata aactatgcca cagataaaaa taagaggaat
    11401 taatgaaaat gatatatgta aaataagtga aaaaatgata aatgatttag ttgaagctgt
    11461 aaaatgtcca agagactacc ttgaaataga atgtataaag tcagttgcaa taagagatgg
    11521 taaaatagca gatgtatatc cttttgttga agtagcttgg tttgatagag gacaagaagt
    11581 acaagatata gtagcaagaa taataactga tagtataaga aataatttag atgtagaaag
    11641 tatggattta gcatttacag tatttgaaaa agaaaaatat tatgagaatg gagagcattt
    11701 ttaatggcta aaaagcaacg tatagataaa attctttcaa atttaggtta tggaagcaga
    11761 agtgaaataa agaagtattg taaacaaggg tctgtagtag taaatggaag tgaagtttca
    11821 aatccaggaa ctcaagtaga cactgaaaat gatgagatat tatttaatgg agaagaagtt
    11881 atatatagag aatatatata tttgatgatg aataaacctg atggatatat atcagcaact
    11941 acagataaat atgacccaac agtattagat ttaattgatt tatcatactt agcttttgaa
    12001 ccatttcctg ttggaaggtt agataaagac acagagggat tattagtttt aactaatgat
    12061 ggaaagcttt ctcatagagt tttatctcct aagaagcatg tgcctaagac ttattatgca
    12121 aaaattgatg gagtagttac tgaagaagat gtagaagctt ttttggaagg tgtagtgctt
    12181 gatgacggat ataaaactat gccatcacag ttaaatatat taaaaagtga tgatgaatca
    12241 gaaatagagc ttacaataca tgaaggtaag tttcaccaag taaaaagaat gtttgaaagc
    12301 gtaggcaaaa aagtagtata cttaaaaaga ttgtctatgg gcaatttaaa attggatgaa
    12361 agtttagagt taggtgagta tagagagtta acagatgaag aagtaaaatt gattgaagaa
    12421 agataataaa gatactatag taaattggag gaatgtctgt gaagaaaaaa gatgttatag
    12481 aatttgaagt agataaaatg gaatttggtg gtacatcttt aagccaagtt ggagatagag
    12541 ttatccatat gaagggtgga ataagtggtc aaaaggtgag agctggagtt aaaaaagtta
    12601 gaagtaagaa agctgaagtt aagatgatag aattattaga gaactcacca cttgaaactg
    12661 aagaaacttg caagcacttt agagagtgtg gaggatgtac actattgtct gtgccatatg
    12721 aaaaacaatt agaaataaaa gaaaaacaag ttatggattt atttttaaag caagatttat
    12781 ttggatttca gtttttaggg atagaaggaa gtccagaaaa caaatattat agaaataaga
    12841 tggaatatac ttttggagat gaagtaaaaa atggaccttt gacattaggt cttcataaaa
    12901 aaggaaagca tatagatata caaactgttg aggagtgtat gttggttgat gaagacttct
    12961 caaagatatt agtttctagt gtagagtttt tcaatgaaaa aaagcttcct tattatagaa
    13021 caatgaatca caaaggatat ttgagacatc ttgtagttag aaaagggatt catactaatg
    13081 agattatggt taatatagta acttcttctc aagaata
//
LOCUS       DACXMZ010000101        12992 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.102, whole genome shotgun sequence.
ACCESSION   DACXMZ010000101 DACXMZ010000000
VERSION     DACXMZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12992
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.102"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            68..949
                     /locus_tag="JD016_11145"
     CDS             68..949
                     /locus_tag="JD016_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAU5000191.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLRERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1157..1996)
                     /locus_tag="JD016_11150"
     CDS             complement(1157..1996)
                     /locus_tag="JD016_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HAU5000192.1"
                     /translation="MSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEYKYAVSQGD
                     YVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDELPLFLSRL
                     ECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARIVIESRDDV
                     SNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIADILCNPLS
                     RARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIVEPVGPWLE
                     KMR"
     gene            complement(2175..4109)
                     /locus_tag="JD016_11155"
     CDS             complement(2175..4109)
                     /locus_tag="JD016_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HAU5000193.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEVDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYNST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4221..5234)
                     /gene="galE"
                     /locus_tag="JD016_11160"
     CDS             complement(4221..5234)
                     /gene="galE"
                     /locus_tag="JD016_11160"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAU5000194.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHIVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5258..6133)
                     /gene="galU"
                     /locus_tag="JD016_11165"
     CDS             complement(5258..6133)
                     /gene="galU"
                     /locus_tag="JD016_11165"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAU5000195.1"
                     /translation="MQMTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIE
                     EAIESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHY
                     IRQKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGV
                     QKIAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSIL
                     EKQTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENL
                     KDDLMDYMRKKVSEK"
     gene            complement(6325..6645)
                     /locus_tag="JD016_11170"
     CDS             complement(6325..6645)
                     /locus_tag="JD016_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000196.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6635..8626)
                     /locus_tag="JD016_11175"
     CDS             complement(6635..8626)
                     /locus_tag="JD016_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000197.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8749..11292)
                     /locus_tag="JD016_11180"
     CDS             complement(8749..11292)
                     /locus_tag="JD016_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HAU5000198.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESVSQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGVKEEIDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQVKSEEMEQNEL
                     KEIKQKEPSQHIEEERNVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11570..11904)
                     /gene="rnpB"
                     /locus_tag="JD016_11185"
     ncRNA           complement(11570..11904)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JD016_11185"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12036..12713
                     /gene="srtB"
                     /locus_tag="JD016_11190"
     CDS             12036..12713
                     /gene="srtB"
                     /locus_tag="JD016_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HAU5000199.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(12873..12947)
                     /locus_tag="JD016_11195"
     tRNA            complement(12873..12947)
                     /locus_tag="JD016_11195"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12913..12915),aa:Thr,seq:tgt)
BASE COUNT         3990 a         2285 c         1332 g         5385 t
ORIGIN      
        1 aaaatatctt aaaggttaac ttctacaaat atataaataa aaatacctat gaaaggatga
       61 acaaaaattg aatttatatc acttacgcta ttttgttaca ttggcacatt tagaacacta
      121 tacaaaagcg gcagaaaatt tatctataac acaaccaagc ttaagtcatg ctatttcttt
      181 actagaaaat gaacttgggg tggctctatt tgaaaaagaa ggtagaaata tagtccttac
      241 taagtatgga aaaatatttt taaaagatgt ggaaaaatca ttagaaattt tagattcaag
      301 tgtaaaatca ttaaaaataa ctggtactgg tgaaggacaa atagacttag cttttcttag
      361 aacacttggt actgatttta tcccagacat agtgcataaa tttttaaaat ctaatcctgc
      421 taaatcaata gattttaaat ttcatactgg tgtaactact gacattattc aaggtcttag
      481 agaaagaaaa tatgatattg cattttgttc gaaacttgaa aaagaaaagg gaattgaatt
      541 tatacctgtt gccaaacaag atttagtact aattgtacca tatagtcatc cactagctgc
      601 aaaggatact atagatttaa aagaaaccat accttatcct caaatagtat ttaatcagag
      661 aagcggtctt agatatataa ttgatgatat gtttaaaaaa ataaatcaac agcctaatat
      721 agtctatgaa gtagaggaag accaagtaat tgctggatta gttgcaaaaa atttcggaat
      781 tgctgtagtc ccaaatatga atatgcttag ctttacaaaa gtaaaagtaa ttcagattat
      841 tcacccaagt tgggaaagaa acttttactt agcatttata aaagatagat atttgcctcc
      901 tgcaattaaa aactttaaaa actttgtcat taaaaacgct caattataac ttattaataa
      961 aggggttatt tttaaatatc ctcttttatt ctttttataa aataaaaaaa tctatacttt
     1021 taaaagtata gattcctaac tgacagtcgg catccgagtc tcagtaatta tcgttataca
     1081 acagcaactg gattcagttc ctgaataact aggttactta cattatatca gattttttaa
     1141 aatttgtcaa ttgtatctaa cgcatttttt caagccatgg tccaacaggc tctacaatag
     1201 gaacttcttt aaatccccaa tttcttatat cattcacact cgttggagta tatattttgt
     1261 ttctgacaga tttgaatatt ttgttttttt ctccatattg catacacttt ggattagctt
     1321 ctattaaccc tcttcttgct ctgctaagag gattacataa aatatctgct atttctaaac
     1381 cttctctata gtcagcatct tcttttttgg caattataaa acccttaacc ttatctctta
     1441 attcttcatc tatatctaag tatattgttc cattattaaa tacatcaaaa aacgcctttt
     1501 gtacttttaa gtttgagaca tcatcccttg actctataac tattcgtgca gattctacag
     1561 tttcattatt tacaaatgaa taaaaattct gaagaacatg tataaaagcc acaacatacg
     1621 gttcctttga aggtgcataa tatttcttta atctagcttt atctactgtt actgatatta
     1681 tagtacactc taatctagat agaaataaag gcaactcatc atagaataac ttaatctgtg
     1741 aatcatcaaa cttctcaaat gctcttgatt ttcttgatat atctgtcaaa tgtattccta
     1801 tgttactggt tccaaaacaa tactctttaa atgcatccat ttctttcctc aaagaacata
     1861 cataatcgcc ttgacttact gcatatttat attcaaaaat tactcctgtc aatgtaaatg
     1921 ggtcttcatg atttatatca gctcttccac tttcatcaat aaacattata tattttttct
     1981 ttataatctt actcaccctc ttaacatatg atgatagcat ctatattcat tttatatgag
     2041 ttttaaaaat attccaagat ttttatataa cttaaactta attttatact aaaaaattga
     2101 gatgtaaaac aatcaagctt ttattgattg aatttcacat ctcaattata attatttttc
     2161 aatatatatt tttactattt tgcaagaata tccattaatt tatccattac tatataagat
     2221 accccgcctc ctatttgata aggagtttca acttttttat ttgcaaatac tctttgttgc
     2281 tctgatgata atcctgaatt atccattaaa actattggtg aatgtaattt tacagctaat
     2341 ggtccagctg ataaagcatc tactaaagca tctgatttac taactaatac agcctgtaaa
     2401 tcagtatctc catagaattt ttctattact ttggcattag tatctactct actatctcca
     2461 tatattctat ttcctgaaat atcttcagta gttatatcat tcatttttgc tattactgaa
     2521 tcgttaattg cacttggtcc acctataaag tatgcatttt ctaattttct ttctgttagc
     2581 catttataag attcttctgt aattccatct ttattgacta atattatagg ttgtttttct
     2641 tccccagctt tagaagctac agataaagca tctgcttccc catttccaga aaccatatat
     2701 gcagttttta tttcttctcg atttccaagt tcttcagcta ttcttgtagc agtttgatat
     2761 ctatcttgac ctgctattct ctttgtttct gtgttgaaag tagtttttat ttcttgctct
     2821 aatttagaac ttattgcatt ttctccacct atcaagatta ctgttttagg tgctaataac
     2881 ttcatttctt caacagtttc agttggtaaa ttagctgtat tagtaagtag tattgttgag
     2941 ttatagctac ttgctaaagg agttgctgtt ataccatcag ctattgcatt accatttact
     3001 aatattacag tatctgagct atcagcattc cagtttccaa ctgaacttat ctttttagct
     3061 gtatcaaatc tatttgcacc tataattgta tcccatgtag gatttatagt tccatcttca
     3121 ttaaaagtat aatctttccc atctattttt tgagaaccag ttagcatctt tccatctttg
     3181 tctaaatagt aagacttacc atctatttgt tgccatcctg tctgcattat tcctgttttg
     3241 tcaaagtaat acttattacc atctacttct tgccatcctg ttactgctaa accatcagat
     3301 cctagatagt atttctttcc attcttttct tgccatcctg tttgcattac tccatctgag
     3361 ttaaagtaat aagtttttcc gtctattact ttccatccag atactcttgc tccatcagtt
     3421 cctaaataat attttttgtt attaattgtt ttccatcctg tttgcattac tcctgaagca
     3481 tcaaagtaat acatttttcc atttactttt agccaactat tcttttggta tgttccatca
     3541 gattttacat aatacttctt accattttct gttttccatc ctgtttgatt tagatttatc
     3601 ttatattttg cggctatctt tacatagctg tcattactat aatctataat tacatctgaa
     3661 cctacaggaa cttggtcaaa taaccatcta acatctttat tgtgcattct tatacatcca
     3721 ccagaaatat gctttcctat agaactctca ttattatttc catgtattcc atatgtatct
     3781 ccataagttc cttttaatgc taatcccatc catctatctc ctaatggatt tcttggactt
     3841 cctccaggta tattaccttt ataatatggt ctatttttta tcttgttaac tacttttgtt
     3901 ttccctgttg gtgtttctga ccccttttta cctgttgcaa ctctaaattc ttttacaagt
     3961 ttattattta caaaatatcc tagagtattt tttcttgaat ttactataat cacatgttta
     4021 ctggctgcgc ttgattctgg tacgccacat gcaaatacta aaactaaagt cagtgctagt
     4081 actaaatact tttttagact ttttttcact ataacttccc cctcttttag aaacattact
     4141 acatgtgtct aattataaca taaaattacc aataccttat atttacaaat ttgttacatt
     4201 tatacatata agtatatatt ctattcttca tacccattag gattcatact ttgccatctc
     4261 catgaatctt cacacatctc ttcaagttca tacttagctt tccatccaag ctctttttct
     4321 gcctttgaag aatctgcata acacatggct acatctccag cccttcttcc tacaatctta
     4381 tatggtattt cttttccact tgctttagaa aatgctttta ctaaatcaag aacactatac
     4441 ccctttccag ttcctaaatt ataaacaaca agtcctggat tttcttctaa tttttgtaat
     4501 gcttttacat gaccagcagc taaatctaat acatgtatat agtctctaac tccagttcca
     4561 tcatgtgttg gatagtcatt accataaaca ctcaattcct tcaatttacc tactgctatt
     4621 tttgttatat aaggcattag attgctcgga actccatttg gttcttcacc tattctacca
     4681 cttttatgag caccgactgg attaaagtat cttaaaagag ctatatccaa tgacttatct
     4741 gcttttgata tatctactaa catctgctct atcataagtt tagttcttcc atatggattg
     4801 gttacagaaa gtgggaaatc ctctaatata ggacaagtat gtgggtctcc atatacagta
     4861 gctgaagaac taaatacaaa ctttttaaca ccatattctc tcattaattc aaataaattt
     4921 aaggtactta ttagattatt gctgtaatat tcaattggtt tttctactga ttcaccaact
     4981 gcttttaaag ctgcaaagtg tataattgat tcaatattat tttctttaaa tacaatatgc
     5041 atttcatctt tatttctgat atcaatatta taaaatttta ctggtttttt agataattcc
     5101 ttgattctat caaccactat tgagttgcta ttacttaagt tatcaactat aacaacttca
     5161 taacctgatt ctaaaagttc tattgctgta tggcttccta tatatcctgc tcctccagct
     5221 actaaaactg ccattgtaat tacctctttt ctaatattta tttttcactt actttttttc
     5281 tcatatagtc cattaaatca tcttttaaat tttctctctt tagagcaaag tctattgttg
     5341 cctctaaaaa accaatctta tctcccacat catatcttct accttcaaag ttataagcat
     5401 aaatagcctc ttgttttcca agagtttgaa gtgcatctgt cagttgtatc tctccacctt
     5461 taccaggtgt ctgtttctct aatatgctaa atattgcagg agttattata tatcttccaa
     5521 gtatagctat atcagatgga gcttcttcaa ttgcaggttt ttcaaccata tctttaactt
     5581 tatatactct atcttcgata tgttttacat ccaatatacc atatttatta gtatcttctt
     5641 tagctatctt ttgaacacct aatacagttg ttttatactc attataagta tctattaatt
     5701 gttttaagca cggaacatct gaatctacta tatcatcacc taataatacg gcaaaaggtt
     5761 catcacctat aaaacttttt gcacagtaaa ttgcatgtcc aagacctttt ggctcttttt
     5821 gtcttatata atgaatattt accatatttg atatgtctct taccatttct aacatctctg
     5881 tcttaccttt ttgctctagt tctaactcta attcaactga tttatcaaaa tgatcttcta
     5941 tagatttttt atttctacct gttattataa gaatttcttc aattcccgat tctatagctt
     6001 cttctattat atactgtaaa gttggtttat ctactatagg aagcatttct ttgggctgtg
     6061 attttgttgc tggtaaaaat ctagttccta gccctgctgc tggtattatt gcttttttaa
     6121 ctgtcatctg cattttttca cccttcttcg taaattttct attgtaccaa attaatatac
     6181 catatttata ctttttcttc aaactctata ttatcccaat atttaatgct ataagataat
     6241 ataaggtaga tgaaatttca tctaccttta gttttataag ttaatattgt attgtttata
     6301 ttttatccaa aacttataca ggtactaaat ttcatctaag atgcctttta tatcactatc
     6361 tatatcttca tctagataag aatctaaatc ttctaagtca acttctagtt cttgatatgt
     6421 catatcaaca ctgacatcct catctatatc catttctgat attaattctc ctaaaatact
     6481 atttccatca gaatctaaac cactatctat atcttcatct atatagtttt catttaaaat
     6541 ttcatctaaa tcaatctcgt aataaacttc attatttttt tcatcccctg agtccaatcc
     6601 taagaactca tagttaaaag tccaaacatt gtatttactt gacatacttt attcctcctg
     6661 tgaaaaacat attttcatct agtttattgg ttccataatc aacagagtta tatatttttt
     6721 caattatgtc ttttcttctt tcttttgtac tattatttaa tgtcaattgt ttaaatacat
     6781 atgtatttgt tattctctcg aataatattt cttttatact atcatcatca atatttctat
     6841 agtttttcca caataactca aataacttaa acatttccct actagtttca tctacataat
     6901 aaggatacat attttctact aaatggttta atcttgatat ggcttgtttt aaaaatacat
     6961 caagacttgc actttcactt tcttttaaca ttctataatt gtatttcttc ttatcttctc
     7021 ctgaaattac aaagatgata ttgtctaaac ttttttcaac ttcatttaaa taactgctta
     7081 tattatatga taagtcagac aatagatttt taatctgctt aaacacttgt acatcaagtt
     7141 tagtattata cttttcaaac aaatctattt cttgattttc taatattctt ggcatatatg
     7201 ctgacaataa atctaaattg tttctttgtt cttgtatttg cttataaagt gaatttataa
     7261 cttctattct tatttcatca tttaactctt tttctaaaat cttatcaaat tcagatttca
     7321 tatagtaaat atttccaaag aaattaaata ttatcatatc attcatagct ttagaccaat
     7381 tcttatatat cacttcaata gaattttctt taagtagagg ttgctttgca tatataaata
     7441 catcttgcat aagtttttga accatatttt tacttaagtt taaaagtact ttttgattat
     7501 taaattttat ttccgtttct acaaaattag aattttcgtt ttcaagttct ttattaatta
     7561 caatgtcttt tatatcacta ttttttatag atatgatttc tttttgaaaa gactttagaa
     7621 tagtcaagct atcgaaatct tcttgaataa aaaatggagc atgttttaat gtatatccca
     7681 ctatttcatt ttctatatct tttaggtcat ctatttgcat aatttctcta tttttatcat
     7741 ttatatgtag gttaaataca tttttattat catataatat tatatcttct cctaaattta
     7801 acttagaaca attatctttc gctgacttta caatagtctt cttagatttc ttatttataa
     7861 aaattaattg gtcttgatat tttactaaaa gcatgtttgt gtttttataa tcaaatccat
     7921 ctatatttcc tgatatcttg cagaatacta tttcacaatc tttaggaaga tttcctatct
     7981 tttggttatc tttcactctc tcatttaagt cagatgggac tacactatct atattgttat
     8041 taaatataga tacagacctt actataatcc cctctctttc catatgaaag tatcccttca
     8101 atgacactct tttatcttct aaaatgtatc tttctatatc atcaaaattt atttcaaatc
     8161 tatttttccc actgataaat ataaacttct cattatcttt attaattcca cccatataaa
     8221 acttattgtc atctaaactt aatatagtca tattatcctt tagatagaat ataaattctc
     8281 tttgactttt gcagattaaa tcatattcat ccaaagttat tttcatagtc aaatttgctg
     8341 taacattttt acttgaattt tcacttttta tgtaaaaagg caagttattt ccatagacaa
     8401 ttatttgaga atcttctaaa actgcctcta atttttcttt attgtctgaa aaactatata
     8461 tatattttac acctgtatcc tcattaactt catctgattt gctgattatt atattcccta
     8521 gatttgttat atgaagcttt ttatattctt ttgagtatct acttatgcca ctaagctcaa
     8581 acccatctga aagctcatat atgatttttt ctcctaattt caacatacct tcaccaccta
     8641 aacagaatta taagtatttt ataaaggtga ataaaatgtc tcaaaacaaa taatattttt
     8701 tttaatactt tttattctga gacatagttt ctcctttttt ataatgctct aagcaccttc
     8761 aactatttct tcataaagtt tatctaaatg tttaacatat tcacgatatc cagatttgtt
     8821 ctttaactca cttcttaaat cttcatattt ttgtttaagt tcttcagcac tcaaagaagg
     8881 agctgattct tttaattctt ctaacttata atcatagtac ttatcataca tagctatata
     8941 tgtatcaata gacttcttag ctagttcatt acatttttct tgtgcttctc catattgaga
     9001 ataatcacct atttttattt tatagtatcc attttcttga attattgttg aagaaatacc
     9061 tttagcctca tattcttttg ctgtttcttt tgctgttttt tcaactttaa gtgaacctgc
     9121 ataaacagtg taatatacac ctttaccatc tttatatgct tccatttctt ttgctttctt
     9181 ttcagcttgt gctttttctt gagcctcttt ttctgctttt gccttttctt ctgcttcttt
     9241 tttctctttt tcagcttgtg cttttttctc ttcttcagtt aatttattgt tagcttgttg
     9301 ttgagttgat gactttggaa tattctcttt atcatttttc ttattgttgc ctactgttat
     9361 aaaatatcct acaccaacaa taaccactat aactactgct ataatagttt cttttaatct
     9421 actcttctta gaaacaacct cttcatcatc taaagaatct tctttatctg atacataatt
     9481 tttcatttct ttcttgtttt ctctatattt gtccttagaa gaatttttag attttttatt
     9541 actattttct ttctttttaa taggtattac tactgatttt tcattttcta atttttcttc
     9601 ttttatctct tttgcttttt tatcttctaa gtttttactt tctttttcaa gcttcgattc
     9661 attatttgaa gatacttttt catctaagtt attatttatt ggtttttcta tttttacatt
     9721 acgttcttct tctatatgtt gacttggttc tttttgtttt atttctttta attcattttg
     9781 ctccatttct tctgatttta cttgatgatt ttcaactggt ttatctaaaa ttatatcagg
     9841 agaaatatca tttaaattct cttcttcttg ttcaacttct tcattgtctt ttttaataaa
     9901 tccaaataat ttagccttct ttttactctt tttattttct ggttcaatta tatcttcttt
     9961 attgctgtcg tactctttat ctgtagtatc tttgtcttct acaacgtcac ctatatcttg
    10021 attattacta tctatttctt ctttaacccc ttcttcagca tcatcttcta ttgaaatact
    10081 acttctaaag caaatatctt tatctgcatt ttttatcatg taaatgtatt cttcaatatc
    10141 gatatcttta tttcttctag ttctttttga tgcaatttca tgattttcta attcactata
    10201 gttatctttt attagattct gtgctttatt ccatgtatct tcactaattt ctgttttatt
    10261 tttaattaca tagtccacaa attccttaaa attttcagga ccaaattctc caattatagt
    10321 cagtctcatt gcaccttcta taaggtctct atttcttctc tttaaaaatt ctaatacaac
    10381 cgttgcacaa gcaaaatctt taataatttc tttgccaaaa ccatatcctt caacagaatg
    10441 aagttcttct aatccacata acttcttata ttcattgtgt attgtttgag atacactttc
    10501 attatttaag ctatttaact tatctataaa cttcgattct atatcatttg attttgattc
    10561 atgttttact tttgaatttt taatttcatt tagtagcatg tcattttctg aattatcttt
    10621 acctaattgc aaatcctcaa tttcagttgc ttttatttct actttgtctt gttcgttatt
    10681 ctcttccaca ttattaggta ccacatcttt attgctagct aatattttgt ttacacagaa
    10741 tataatttct ctttttaatt ctcttttcgc atctgcttga tttttaagaa ataaatctcc
    10801 acattctttc ttttctaaac ttatttttct aagagatata ttaagaaggt ctttatatag
    10861 gtttaaaacc ttataatcct tttctaaacg tcttcttctc tctgatagtt ccattttatc
    10921 ataagctaaa aaactattta aatcattggc ttttagagag tctaatccct ctttcatagc
    10981 cttatttaca tactttctac attttgctgt agttaattct aaatcttttt ctaattcatc
    11041 tatttcaggt atgtacttat ctactatcca tttgccaata agtggttctg tatttcttgc
    11101 taatgtctta taaaacaata tattaaattt taaaagctct gcatattttg cttcattaaa
    11161 ttctactttt tcaaatttct tttccaaaac gccatctagc ttttcattta ttttagactc
    11221 ttgttttaaa aaacttgctg tgacatttaa aacagtgttt cttattatat tattttcact
    11281 caaaaccttc actccttaaa tcaattattt tattgtaata tttacattgt gcaaagttgc
    11341 taatttgatt atgatatcta aataattatt tactaaattt gctacatctt atatcatatc
    11401 atattattta catcatttta ctttttcttt ttatatctat cttatattat accattttta
    11461 atatctgtac taacttatat tacaatattc ctcaaaattt taaaagtaga ttagacaaat
    11521 ttacaaataa aaaaacatca gtttaaactg atgtttttga ctatcgaaat aattaagcaa
    11581 gtctataagc ggagttctgt attagataat catctttcta ggcctattgt caccaatagg
    11641 ctcaagcgac ctacctaccg gagagtacga gcagcactct ttattcctat cttggtcttg
    11701 ctccaggtgg ggtttacata gccaactagt cgcctagctg ctggtgagct cttacctcgc
    11761 ctttccatcc ttaccacaat agtggcggtt tatttctgtt gcactatcct taggatcgct
    11821 cccactaggc gttacctagc accctgctct atggagctcc gactttcctc atgtaagatt
    11881 acatgcgatt acctggctta cttaatattt attttactta gttatgttaa cacaaattct
    11941 tttacatgtc aaagtaatat tatactaaaa atttaaattt attcaaaaat agtgtaatat
    12001 agatatataa actacattta actcgaggtg atatattgaa aaaattatat agaatagtaa
    12061 ttaacattat actagtatta gtaatacttt acagtggatt taatatatac tcaaaactta
    12121 ctaaatacaa tcatgatact aaaatatcct cagaacttca aaaaaaagaa tataaaaaag
    12181 aggatttatc aaaaattaac agtgatttta aattttggct ttctgttgag aatacaaata
    12241 taaattatcc agttgtacaa tctaaagata attcttacta tcttgacaaa gatttttata
    12301 aaaaagattc tatttctggt acactgttta tggattatag aaataaatct attgatgaca
    12361 aaaatatcat aatatatgga cataatatga aaaataaaac tatgttcaac aacctaaata
    12421 agtttaaaga tgctgacttc tttaaaaaaa ataataaaat aaaaataacc ttgaatggaa
    12481 aagaattttt atatgatgtt ttttctgctt atattgtaga atctgattat gattatctaa
    12541 aaacaaattt taacaatgaa tctgattatc aaaattacat aaatgatatt acttctaaat
    12601 cattgtataa atctcctatt aaggtaaata gcaatgataa aatagttaca ctttctacat
    12661 gtacctatga atttgatgat gccagaatgg tgattcatgg tagattgatt taaaactcac
    12721 agacacagtt taaaacaata attttaaact gtgtcatctt ttatacctat aatttgtaaa
    12781 ttagaccact aaaagtattt aatacaaaat aacaaataca tcaaacaata aaaaacaata
    12841 aaaaaagact atgctaaaaa catagtcttt actggagctg gtgataggaa tcgaacctac
    12901 aacctgctga ttacaagtca gctgctctac cgttgagcca caccagcaca cccattttat
    12961 tgtaaattag taaaactaat ttttaaaagt ta
//
LOCUS       DACXMZ010000102        12903 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.103, whole genome shotgun sequence.
ACCESSION   DACXMZ010000102 DACXMZ010000000
VERSION     DACXMZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12903
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.103"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            37..501
                     /locus_tag="JD016_11200"
     CDS             37..501
                     /locus_tag="JD016_11200"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAU5000200.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKIVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            511..1254
                     /gene="yaaA"
                     /locus_tag="JD016_11205"
     CDS             511..1254
                     /gene="yaaA"
                     /locus_tag="JD016_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAU5000201.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            1382..4039
                     /locus_tag="JD016_11210"
     CDS             1382..4039
                     /locus_tag="JD016_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAU5000202.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKNALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      4093..4195
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4305..5570
                     /locus_tag="JD016_11215"
     CDS             4305..5570
                     /locus_tag="JD016_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HAU5000203.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIEQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            5600..6490
                     /gene="metA"
                     /locus_tag="JD016_11220"
     CDS             5600..6490
                     /gene="metA"
                     /locus_tag="JD016_11220"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAU5000204.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            6981..7424
                     /locus_tag="JD016_11225"
     CDS             6981..7424
                     /locus_tag="JD016_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAU5000205.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            7455..9272
                     /gene="hflB"
                     /locus_tag="JD016_11230"
     CDS             7455..9272
                     /gene="hflB"
                     /locus_tag="JD016_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAU5000206.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            9450..12152
                     /locus_tag="JD016_11235"
     CDS             9450..12152
                     /locus_tag="JD016_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HAU5000207.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            12186..12884
                     /locus_tag="JD016_11240"
     CDS             12186..12884
                     /locus_tag="JD016_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000208.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
BASE COUNT         5069 a         1487 c         2370 g         3977 t
ORIGIN      
        1 ataaaattat taataaaaac aaaaggagag atatttatgt tgagtatagg aacaaaagca
       61 ccagaattta cactagaaga taaagatgga aataaggtaa gtatgtcaga ttttaaaggt
      121 aaaaaaatag tagtatattt ttatcctaaa gataatacac caggatgtac acgtcaagct
      181 tgtgctttta gaaatgcata tgatggtttt aaaaaggaag atatacaagt tattgggata
      241 agtaaagata gtataaaatc acatcaaaaa tttgcagaaa agcatgaact tccttttatt
      301 ttactttctg accctgactt agttgctata aaagcttttg atgtatggaa agagaaaaaa
      361 atgtatggaa aaactgcatt gggagttgta cgtgcgacat atattataga tgaaaatgga
      421 attattgaaa aagtctttga aaaagcaaaa ccagacacaa atgcacaaga gattcttgaa
      481 tatttggaaa aacaggagta ataagaattt atgattataa tgttatcacc agctaaaaat
      541 atgaagaata tagaagtatt tgatagagat ttgagtttgc cttgttttat agataacaca
      601 aaagaaatag tggaaaatat aaagacattt ggtatagaag attttaaaaa taaaatgaaa
      661 attaatgaaa aattagctgt attaaataaa aatagatttg aaagcattaa atttgataga
      721 ttaggaaacc ctgcaatctt aacttatgat ggaatacaat ataaaaatat agaagcagaa
      781 aattttacaa ggaaagatga agagtttgca aatagctgta ttcgaatcat aagtggattg
      841 tatggtgttg taaaaccata tgatagtata tatgaatata gattagaaat gcagacaaaa
      901 ttacgagtag gtgagtttaa gaatttatac gaatattggg gaaatcgtat atataaagaa
      961 ctaatcaaag aaaaaacagc tatcgttaat ctttcatcaa atgaatacag caaatccata
     1021 gaaaaattta ttaaagatag tgatacatat attacatgta cttttaaagt aaataaaaat
     1081 ggcatattga aagtggaatc tacacaagca aagaaagcta gaggtatgat gacaaaatac
     1141 attgtaaaaa ataggataag agatattgaa gaactgaaaa agtttaattt agaaggttat
     1201 aagtataaag aaaacttatc taacaatagt gaatacatat ttgtaaaaga gtagaagttt
     1261 tgttaaatat taaatggcat tgttaaattt aggtaaaata tacttagtgt gtattgaatt
     1321 gaacttgatt ttaaaatata attattctta aattaaaaaa agaaaggagt ggtcttccaa
     1381 aatggaaaat cacaaacaga tgccttggca ttcaagagca acgcatgaaa ttttagaaag
     1441 tctagattca tcagaggagg ggctcaccga taccgaggcg gaaaaacggc ttaaagagaa
     1501 tggtttaaat gaacttagaa aaaaaccacc tagaacaact ttacaaatgc tttggagtca
     1561 aattacagat gctatggtta tgatactaat aggagcagca atattatctt tgatatttgg
     1621 cgaatttaca gaagcttttg taatactaat aattgtaaca gtaaatgcag ttattggaat
     1681 tgttcaagag aaaaaagcag aatcatcatt agaagcatta aaaaatatga attcacctac
     1741 agctcgtgtt atgcgtgatg gtgaagaaaa tgttattcca gcaagtaatc ttgtagtagg
     1801 agatattgtt tttttagaag atggagctat ggttccagca gaccttcgtt taatagaaac
     1861 atcaagttta aaaattcaag aggcttcttt aacaggagaa tctgttccat cagaaaaaga
     1921 tgcagataca atcttaccta aagaatgtgt attaggagac cgtagcaata tggcgtatac
     1981 atcttcaata gtcacatatg gtagaggtgt aggtgttgta gtagcaactg gtatggatac
     2041 agaagtgggt aacatagcac atcttttaga ttcacaagat gattttgata ctcctataaa
     2101 acgtaaactt aatactgttg gaaagacatt aagtgtcata ggtattattg tatgcgttgt
     2161 tatatttgca ataggtgcat tttatcagag acctctcttg ccaatgttta tgattgcaat
     2221 ttcccttgcc atatcaatta ttccagaggg attacctgca acagctacta ttgtaatggc
     2281 tttaggcgta cagcgtatgg ctaaaagaaa tgctttaatt agaaaattac ctgctgttga
     2341 aacattagga ggagctacag taatatgttg tgataaaact ggtacattaa ccttaaataa
     2401 aatgactgtt acacatattg ctgtcaatgg agattttgaa tctggaaaag tgacatcaat
     2461 agatactgca tctgaaaaac atccattagt gtataaagag attgtttatg ctggtgcact
     2521 ttgtaatgat gcatgtatta atccagataa aacagatgaa atattaggtg acccaacaga
     2581 gggcgcatta atttttatgg ccaaaaaatt tggaatagat caagaagctc ttgaagaaga
     2641 atatccacgt gtatttgaac agccttttga ttcagataga aaatgtatga caactgttca
     2701 taatatagat gaaaagatta ttgcttatac taaaggagca gtagacgaaa ttcttgacct
     2761 atgtactaaa atattaactt caaaaggtga aagaaacatt actgaagtgg ataaaaagaa
     2821 tatacacgaa ttatgtctta acatgtctaa aaatgcattg cgtgtacttg gttttgctaa
     2881 gcgtgagatt tcatctatac caaaagaaga tagtgaaaat atagaatatg atttaacatt
     2941 tataggtatg gttggtatga ttgacccacc aagaactgag gtaattgatg cagtaagtac
     3001 ttgtaaagaa gcaggtataa gaaccataat gataacaggt gaccataaag ttactgccac
     3061 aacaatcgct catgagttag gaatatggag tgaagaaaat actgtgatat ctggtgatga
     3121 acttgataat ttgagtgatg atgaactaga tgaagctgtt aaaaatacta cagtatttgc
     3181 tcgtgtatcc ccatttgata aacttagaat tatacaatct ctaaaaagaa ttggagaagt
     3241 accagcaatg actggggatg gtgtaaatga ctctcctgcc ttaaagtctg ctgatattgg
     3301 tatagccatg ggtataagtg gtacggatgt tgcaaaagat tcttctgaca tgattttaat
     3361 ggacgatagt tttacaacaa ttgcttatgc aataaaagaa ggtagacgtg tatatagaaa
     3421 catacaaaaa gttatacaat ttttgcttgt tggtaatata gcagagatac ttacgttgtt
     3481 tgtagcaata ttattaaatt gggatactcc gcttcttgct gttcatattc tatgggtaaa
     3541 ccttgcaact gctacgcttc cagcccttgc tcttggagtt gacccaccaa gcagaaatat
     3601 aatgaagcac cagccagtga aatcaaccac tttgtttgaa aaaaatctta tacaaagagt
     3661 tgttacccaa ggtatatttg tggcaataat gactatttta gcatataaaa ttggtcttaa
     3721 tatagaaaat cattctgtag gtcaaacaat ggcattttgt gtattagcat tttcacagat
     3781 gttacgagca tttagtcagc gttcaaatac agattcaatg tttaatagaa ataatggtaa
     3841 aaatccattt cttttaatct catttttaac ttctgcattt ttaatagcag taatactgtt
     3901 tgtacctgtt tttagaaatg cttttaactt aacagtttta aataacttag agtggcttat
     3961 aacgattgtt ttagccataa tgtctgtaat tcaagtagag gttgggaaaa ttattaaaag
     4021 aaaaaaatta aaaaaatagt tgacaaaatt aggaaaaata tataagatat aaaacataga
     4081 aaaattaaat aaatcttatc cagagaggtg gagggactgg ccctatgaaa cccggcaacc
     4141 agtatattta tatacattgg tgctaaatcc tgcggcgaaa gctgagagat gagtataatt
     4201 aagcttatac agagcctgag ttatatactc aggctttatt tattttaact ctcatctggt
     4261 aaatagttta atttttttta ctaaaatcta agggggatac aagaatgtat aataaagaaa
     4321 caatatgtgt gcaaggaaat tataaaccag gtaatggaga accaagagta ctacctttat
     4381 atcaaagtac aacttttaaa tatagcagta tagaacaact tgctgaatta tttgatttaa
     4441 aagttgatgg acatatatat tcaagaataa gcaatcctac tattcaagct tttgaagaaa
     4501 aaataagttt actagagggt ggagtatctt ctgtagctgt atcatcaggg caatctgcaa
     4561 atatgttggc agttttaaat atatgtaaat caggagatag tatactttgt tcttcaaaag
     4621 tatatggagg aacattcaat ttactaggac ctagtcttaa aaaatttggt atagatttaa
     4681 tatcgtttga cttagattca agtgaagatg agatagtaga acttgcaaag gaaaatacta
     4741 aggttgtgtt tgcagaaaca cttgcaaatc caactcttga agtcatagat tttgaaaaaa
     4801 tagcaaatgt agctaagaga attaatgttc catttattgt tgataattca ttagcatctc
     4861 cagtgctttg taacccttta aagtatggag caaatatagt tactcattct accacaaaat
     4921 atttagatgg gcatgcttca agtgttggag gaattatagt ggatggtgga aactttaact
     4981 gggataatgg aaaatttcca gaattagttg agccagaccc aacatatcat ggaataagct
     5041 atactcaaaa atttggaaat gccgcatatg caactaaagc aagagttcag ttgcttagag
     5101 actatggaaa ttgtttaagc ccattcaatg cgtatcttac taatttaaat gttgaaacac
     5161 tacatcttag aatggagaga catagtgaaa atgcacttaa aatagctaga tttttagaaa
     5221 aacatgaaaa tgtagattgg attaattacc caggacttga agataacaag tattatgaga
     5281 atgccaaaaa gtatttatca agaggatgta gtggtgtttt atcatttgga gtaagaggtg
     5341 ggttagaaaa tgccaaaaaa tttgtggaaa aattacagat agcatctttg gttacacatg
     5401 tttcagatgt aagaacttgt gttatacatc cagcttcaac tactcataga caattaacag
     5461 aagaacaatt aattgcatct ggagtattgc cttcactaat aagattatct gttggaatag
     5521 aaaatgtaga ggatttaata gctgatttaa atcaagcttt aaatttctaa tattctaaga
     5581 gaaagagaag tgaaaagata tggctttaat actaccaaag ggattacctg ttattaataa
     5641 attattagat gaaggaatag atgtaattta taaagaagat tttaaaaagg aattaaagta
     5701 tgaagaaaac atagacacta agatagctat actaaatcta atgcctataa agatagatac
     5761 agaattagat ttactaagaa ggatagataa aacaggattc aacgtgtctg tggaatttat
     5821 taaaatttca acaagagaaa gtaaaagaag ttgtaatgaa tatgttaaag aattttataa
     5881 aacatttgat gaagccaaag gagagtattt tgatggcttt ataataacag gagctccagt
     5941 tgaacaaatg gagtttgaag aagttgatta ttggaatgaa ctagaagaaa ttatggatta
     6001 ttctaagaga aaaactaaat ctacattata tatatgttgg gcagctcaag caagtcttta
     6061 caaatactac aatgtaaaaa aacttccact aagtcaaaaa tgttttggag tatttaagca
     6121 taaggtagat aaagatagta aaatagtaga tggatttgaa aatgaatttt ttgctccaca
     6181 ttcaagacat actactgtaa atattgaagc tttgaaagag aataaagagt tgagcattgt
     6241 gagtcattct aaagaagctg gtccctatat aattacaaat tcaagagatg tgttcgtaat
     6301 gggtcatagc gagtatgaca aatacactct tgataaagaa tataagaggg atataaataa
     6361 aggagataaa atatctatac ctcaaaatta ctatattaat gatgaccctt cagaagagcc
     6421 aactgtaaaa tggaaaaaac attcagaatt gttgtttaga aattggataa aaaactattt
     6481 gatacaataa attttaagag tatataaatg ttaactaaaa aaatatagaa atttataggt
     6541 ataaagttaa tcttaatgat actttatacc ttattttttt aaaatttgac cttataatag
     6601 tagttttata ttttaagaaa atcactttag gtgtatgtag atagaggtgg ttttaatttt
     6661 atatatggtg cgataatggg agactaaacc tttatagtat caataaattt gtattttaac
     6721 ttttaaaaga gattaaaaag ggagaaatca gtgtaaaata tatatataga ttttttatta
     6781 aattttatta aaaatatcct gtataaaatt aaatatcaat aagataacaa aatttcaatt
     6841 gaagttacaa tattgtaatt gacaataaat acttaatact ataatattta atacattaaa
     6901 tattatagta ttaagtattt atttacagta tgcacttcag cctaaatttg aaataaagta
     6961 ttttcgtagg gagcgtttgt atggatgaga tgagttttaa gaaagagcta ctagagttga
     7021 ctagagatat aaatatgaaa tttacaactt tattaagtga tttttatcag ccacttggta
     7081 ttacagcagt gcaagctttg attttatctg agttatgtga acatggagag aaaaaaataa
     7141 gcgatttagg gaagaactta aacatgacaa atagtaatgt atcagtaatc tgtcaaaggc
     7201 ttgaaaaaaa tggattttta aatcgcataa gagatataga agaccaacga attgtaaaag
     7261 taaaagttac aaataaatca ttggatatac aagaacatat ttcaagtagt atttttgata
     7321 gttattttga aaatatgacc agtgaaaaat tgcaagatat ggaagatata atagaaggtt
     7381 tagagaaatt aaataaactt ttaacttaca ttgattgcaa ataacataaa aataatttaa
     7441 aggagaataa aattatggat gaacataaac cccaaaaaag gtcagcactc tattattata
     7501 cagttataat aatagtagta atgtttttaa atgctttaat atttccaagt ttaatggata
     7561 aaaaaatggt tgagattgac tatggaaagt ttctcacaat gttggataca ggaaaggtta
     7621 aatctgttga gatagaaaat aatagaatag caataaatcc atctagtagc aaggataaga
     7681 atatatacat aactggtaga atggatgacc cagaattggt agatagacta aaaaatgcaa
     7741 aagtagaatt tactaaggtt attcctaagg aaaattcacc tcttttaagt attttactta
     7801 catggataat acctataggt attcttatgc tatttggtaa cttaatgatg aagtctatgc
     7861 aaaagcgaat gggtggtaat gctatgcaat ttggaaaaag caacgccaaa atgtatgtat
     7921 ctgctcaaag tggaaaaaga tttaatgatg tagctggtca agatgaggca aaagaagctt
     7981 taactgaaat agtagatttc ttacacaatc cagaaaagta taaaaagata ggagcacaaa
     8041 tgcctaaagg agcactttta gtaggacctc caggtacagg aaaaacactc cttgcaaaag
     8101 ctgtagcagg tgaagcaaat gtaccatttt tctcaatatc tggttctgag tttgttgaga
     8161 tgtttgtagg aatgggagct tctaggattc gtgatttatt taaacaagca aaagaaaaag
     8221 ctccatgtat agtgtttata gatgagattg atactattgg taaaaagcgt gataatggaa
     8281 gtggattagg tggaaatgat gaacgtgaac aaactttaaa tcagttgtta agtgagatgg
     8341 atggttttga tggtggaatt ggagtagtaa tacttgctgc tacaaataga cctgacagtc
     8401 ttgacaaagc attacttcgt ccaggaagat ttgatagacg tataccagtt gaacttccag
     8461 accttggagg acgtgaagct atattaaagg tacatgctca aaatgtaaat gtaagctcag
     8521 atatagatta taatgcaatt gcaaggtcta catcaggagc atcaggtgca gaacttgcaa
     8581 atattgtaaa cgaagcagca cttttagcag ttaagtctgg cagagatata gtagaacaac
     8641 aggattttga tgaatctgtt gaaactgtaa ttgctgggta tcaacgtaaa ggagctgtaa
     8701 tttcagataa ggaaaagaaa attatagcat atcatgaaat aggtcatgca cttgttgctg
     8761 ctatgcaaaa acattctgca cctgtccata agattactat aatacctaga acttcaggag
     8821 cattgggata tactatgcaa gttgctgaag atgagagtgt acttatgtct aaagaagaag
     8881 caattgataa aataactact tttacaggtg gtcgtgctgc tgaagaggtt atctttaaca
     8941 cttgtacatc aggagcatca aatgatatag aacaagctac taaaattgca agagctatgg
     9001 ttactcgcct tggtatgagt gaagagtttg atatggtcgc tcttgaaact gtaagcaatc
     9061 aatatttagg aggagatact tctcttgctt gttcttccga tactgctgct aaaatagatg
     9121 aagaggtatt taaaatagta aaggcttgtc acaaaaatgc aattgaaatc ttagaaaata
     9181 atattgataa attgcatgaa cttgctaatt atttattaga aaaagaaact attacaggtg
     9241 atgagtttat ggaaatactt aacttaaact aaaatgatta tatacaagat aaaaatgcct
     9301 attgttaggc atttttattt tacaactaaa ttttttaaat tcatatttaa atctttacat
     9361 aatcttaatc attaaaaata catcttaact acaattttat atcggaattg atattataat
     9421 agtaatgaat ggtgtaagga ggttttttaa tggatagacc taatcctgat attctattag
     9481 aaaaaattaa aaatgaagaa gaaaaactaa gtagaggtca attaaaaata ttctttggtt
     9541 atgcagctgg agtaggaaaa acctactcta tgttggaatc tgcacaaaat ttaaaaaagg
     9601 tgggtgtaga tgtagtagtt ggatatattg aaccacacac tagaccagaa acattggctt
     9661 tacttgatgg cttggaaatt cttcctgtaa aggagataga atataaatct ataaaactaa
     9721 aagaatttga tttagatgct gctttagcaa gaaaaccaga agttatatta gtagatgaat
     9781 ttgcccattc taatgttgct gggttaaggc atacaaaaag atggcaagat atagaagaat
     9841 tgctgttagc aggcataaat gtctacacta ctgtaaatgt acaacattta gaaagtctaa
     9901 atgacattgt tgagataatt acaaacgtat cagttaaaga gactatacca gataagtttt
     9961 tggatacaaa tacacagtta gaacttatag atgttgaacc tgatgtactt cttgaaaggt
    10021 tcaatgaagg taagatatat aagaaagaac aagctactag agcaaaaaat aattttttta
    10081 taaaagataa cttggttgca cttagagaaa tcgcacttag aaaaacagca gagagagtta
    10141 ataaagaagt tcaaatgacc agactatcaa agggtgatgt aactgtaata cctacatcag
    10201 atacacttct tgcatgtata agtccatctc catcttcagc aaaagttatt agaactgcat
    10261 ccagaatatc agacagtaca tttgcaaaat ggatagcttt atatgttgaa actccaaaca
    10321 cagcaagact tccatttgat gaacaaaaac aactacaaaa taacctaaaa ttagctaaga
    10381 aattaggtgg agaaataatt gttttacatg gtgaaaatat aatagagcaa atacttagaa
    10441 ttgcaaagct gagaaatgta acaaaaattg taataggtag aaatcattca agtaataaaa
    10501 gatttagtaa aagatttaaa aaagatatag ttgacaaatt aattgatgaa gttgattata
    10561 tagatataca tataattcca tataaatttg cctcagatgt taaatacaaa cctaaaaagg
    10621 ataaatcatc aataaaatct aaatttaaaa ttagcaaagt tgattttata aaacttatat
    10681 ttattacttt gatgataagt attttagcat atgcagtaca aagcatggga tttataagag
    10741 aaaatatatt gttaatatat atgttgggtg tagttttagt ttctatgtgg actaaagggt
    10801 atagtacagg tataataagt tctgtattta atatagttct tttaaattat ttttttacag
    10861 caccattata tacattatct atagcagatt caaactatat agtaacactg gttgtattct
    10921 caatagtagg tataataact agcactctta cgtctaagat acagcatgaa gcagaaactg
    10981 ctgctaaaag agaagaaaat acaaaaatga tatatcaaat aattaaggga tttttaagat
    11041 tatcaaataa agatgatata gtaaacaaag gtatagaact tttgtctctt agcttaagta
    11101 gagatgtaat ttgttattta aatgtagata agcataataa atcaaagctt tacaaaaaaa
    11161 atactaataa taaggataaa aatgatttgg atagtgaaga tgaaaaagct gttgcaagtt
    11221 gggttttatc caattcaaca gtggcaggaa atgacacgga tacacttcct ggttcaaagg
    11281 gatactatat accaattgta ggaatgaatt taactttagg agtagtggga atatcatgta
    11341 ttgattcaaa attggataca gaagatataa gcttaattga aacaataatt gctcaaatgg
    11401 caatagcgct tgatagagaa atcttgtcag aagctaaaga aaataccaat cttgagattg
    11461 aaagggaaag attaagaagt aatcttttaa gagcagtttc gcatgacctt agaactccac
    11521 tagcaggaat atctggtgca gtaagtacaa ttataaaaaa caagggtact ataggtcaag
    11581 atataataga tgaattactt agtggtgtat atgaagatac tcagtggtta ataagacttg
    11641 ttgaaaattt acttagtatg accaagatag atgaagggaa attggaagtt aaaaaacatt
    11701 ctgaactggt tgaggaaatt atatcagaag cgttacaaaa aataaaaaaa agaattgaaa
    11761 atgcacttat agatattaat ataccagaac agatattatt tgttcctatg gatgcaaaat
    11821 taatagaaca agttttaatt aatttaatag ataattcttt gaaatattca aaggaagatt
    11881 gtaagataga gataaatgta tatgagaaag atgattatgt ttggtttgaa gtatctgata
    11941 atggtcctgg tatatcaaag gaacttaaaa agcatatctt tgacagattt tttactggag
    12001 aagaaggagc taaagattcc agaaagggtg ttggacttgg attatcaatt tgtaaatcta
    12061 taattcaagc tcataaaggt gaaattatgg tagaaaacaa taaagataag ggttcaactt
    12121 ttaagtttag tttgcctaag gaaaatgaat agttttaaat tttaaaatta gttttggagg
    12181 aagaaatgag taaaaaactt atattgttag tagaggatga taagacaata agaaaattta
    12241 tatctactgc acttttaaca caggattatg atgtcaagga ggctataact ggaaaagaag
    12301 gtatatctat tgctgtctca tattctccag atgttgttct tttagattta ggcttagaag
    12361 atatggatgg aatagaggta ataaaagcta tcagacaatt ttcaaatata ccaataattg
    12421 tagtttcagc tagagagcaa gatagagaca aggtagaagt atttgatgca ggggcagatg
    12481 attacttaac aaaaccattc tccatagtag agttattagc aagagtaaga gtagcattta
    12541 gacattcaca agtagaagct caacaaaaag atgatgttaa atctacgttt gaagtagata
    12601 aattattgat tgattttgat aaaagaaaag ttattgttga tgatgttgaa gtccatttga
    12661 cacctattga atataatatt ctttcattac ttgcaaagca tcatggaaaa gtgcttactc
    12721 ataattttat aataaaagaa atatggggta gtgttatagg gaatgagact aagtctttaa
    12781 gggtctttat ggcaacgtta agacgaaaaa tagaaaaaca acctgcaaac ccaagatata
    12841 taattacaga agttggtgta ggatacagat taaatgatga atagttgtaa tatattaatt
    12901 att
//
LOCUS       DACXMZ010000103        12876 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.104, whole genome shotgun sequence.
ACCESSION   DACXMZ010000103 DACXMZ010000000
VERSION     DACXMZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.104"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            3..755
                     /locus_tag="JD016_11245"
     CDS             3..755
                     /locus_tag="JD016_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000209.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            756..2663
                     /locus_tag="JD016_11250"
     CDS             756..2663
                     /locus_tag="JD016_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAU5000210.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNVYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            2815..3495
                     /locus_tag="JD016_11255"
     CDS             2815..3495
                     /locus_tag="JD016_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000211.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            3489..4508
                     /locus_tag="JD016_11260"
     CDS             3489..4508
                     /locus_tag="JD016_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU5000212.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKKVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDY
                     LSLDS"
     gene            4544..4630
                     /locus_tag="JD016_11265"
                     /pseudo
     CDS             4544..4630
                     /locus_tag="JD016_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
     gene            4948..6099
                     /locus_tag="JD016_11270"
     CDS             4948..6099
                     /locus_tag="JD016_11270"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAU5000213.1"
                     /translation="MYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEIPKD
                     IKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHGQTI
                     FHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEFLLY
                     SDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYDKNG
                     YFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDLIAT
                     VTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVAVLTQDEYG
                     YSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            6200..6508
                     /locus_tag="JD016_11275"
     CDS             6200..6508
                     /locus_tag="JD016_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000214.1"
                     /translation="MEKNQRTDYNIPTISFKSGLILIVSSLVSTVVFPLLLSIFGIDN
                     RFGVVIGNSLITSFAVAYTRYFVETKRGFSLGFFRLYILFALSFAIIGYFWIYKGIHI
                     "
     gene            6770..7078
                     /locus_tag="JD016_11280"
     CDS             6770..7078
                     /locus_tag="JD016_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU5000215.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            7167..8513
                     /locus_tag="JD016_11285"
     CDS             7167..8513
                     /locus_tag="JD016_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAU5000216.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            8746..9843
                     /locus_tag="JD016_11290"
     CDS             8746..9843
                     /locus_tag="JD016_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAU5000217.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            9942..10856
                     /gene="murQ"
                     /locus_tag="JD016_11295"
     CDS             9942..10856
                     /gene="murQ"
                     /locus_tag="JD016_11295"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAU5000218.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            10966..11886
                     /locus_tag="JD016_11300"
     CDS             10966..11886
                     /locus_tag="JD016_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HAU5000219.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            11903..12754
                     /locus_tag="JD016_11305"
     CDS             11903..12754
                     /locus_tag="JD016_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAU5000220.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
BASE COUNT         5230 a         1249 c         2283 g         4114 t
ORIGIN      
        1 ttatgaaaga aatattaaaa ataaaaaata tatcaaaaga ttatggaata aaaggattta
       61 aaacaaatgt attaaaaaat atatctctta cagtcaatga aggtgatttt atatcaataa
      121 tgggtccatc tggtgcagga aagacaacat tgctaaattt aatgtctaca cttgacaaac
      181 aaacttcagg agaaataata ctagatggaa ttaatatatc aaaagtaaaa aataatgaac
      241 tatcaaaatt aagaagagaa aaaatagggt ttatatttca agactataat ctattagaca
      301 atatgaaact aatggacaat atagcacttc ctctagcatt aggaaaaaag aaaagtaagg
      361 agatagaagc taaggtattt tctatagcta aaaaatttgg attagaaaat catttagata
      421 aatatccata tcagttatca ggaggacaaa aacaaagagg cgcagcagct aggtcattaa
      481 ttacaaatcc aacagtcata tttgctgatg agccaactgg tgctttagac tctaaatcag
      541 catatgaact tttagaatca ttagaaaaaa taaacagaga aaataacgct actattataa
      601 tgattacaca tgaccctcta acagcaagtt attcaaatga agtatatatg ataaatgatg
      661 gaaatataaa gtgtaaatta aacaaaggaa atagtagaaa agaattttat ggaaaaatta
      721 tggatatgct agcctctatg ggaggtgaga tgtaagtgag tatacttgga attgcttata
      781 ataattttaa gagtaacata agaacttatc tagcattttt tatatctatg gttttttcag
      841 tagttgtgct aactaatttt gaattattaa gatatggtga tgctataaat gtgttacaag
      901 atgaaaataa aaaatttaca ctatcagtgt tagtagctgt aataatgata ctatcagtat
      961 ttttattttt ctttatatgg tatgcaacaa atatattttt taagaatagg tctaaagaaa
     1021 ttggaatatt gtcttttatg ggattagatt tatatactat aggaaaaata tattttgtag
     1081 aaaatatgct tataggaata agttcttgta taacaggaat aattataggg ataataacat
     1141 ctagattttt tcaagtagtg ataataaagt tatctggatt taatataaag gtatctaatg
     1201 gacttagtat agaagctata ctatatgcta ctttaatctt tatttctata tttataataa
     1261 tggctgtaaa aggttttata acaatatgta gaagtagtgt aataaatcta atcaatatat
     1321 ctaaaaaaca agaaaaaata ccaaaggtag gaataggtat atatatatta gctatagtat
     1381 caattatagt aataggatat ggctactatc tttctaccaa tataagaact ggagatataa
     1441 ctagtgtact acctgttata ggtataataa tagtaggtac ttatggatta ttcaaatcag
     1501 ttatgcctgt tatatttgat attataatca aaaataaaaa aatattattt aatggaaata
     1561 atataattgc aataaacaat ataaactacc gattaaataa aaactataaa acttatgcta
     1621 taatagctat aattataact acaacaatat caactcttgg tgcttcagtt gcgatgaagc
     1681 gtatacataa gaatgcccaa gaacaaagaa atgtgtacac tattagcata gtttcaatgg
     1741 ataaagatga agtgaataaa aaacaaatag agaatataat aaaagatact aataagataa
     1801 agtactcagt aaatccagaa ctaacaattt taaagaaaac agatataaaa gaagcttatg
     1861 atacaacaga tactataatg aaatatagtg atttttgtaa tactcttaaa gtgaatggca
     1921 atgataaaga attaaaagag tatggtgaaa gattagttag tggaaataat gtaatagagt
     1981 taaaaagccc tcaaacgata gcatcaatag ctacaaaaga agccaagata aaacttgatg
     2041 gtcagttata taatatttca aggggagaag taaaagttcc agtactaggg acaggactta
     2101 atcaaagtat tatagtggta aatgatgata catattttaa actaagagat aagggtcaac
     2161 aagtgtattt ttatggtgca aaggtatttc aagaagaaaa tagtcaagta atgttcaaca
     2221 atattgaaaa gaatttaagt ttaaaaaatg tatatattca aaatggatat aaagctcaag
     2281 gtgagagtca atggatgaag tttgcatatg tagtgcttgt gtttttattc ttagtctttg
     2341 caattgtagc tggaagtata atatatatga aaatatatag tgatgcatat gaagataaag
     2401 agaaatataa gattttgtta aaaataggta ctacagaaaa agatataaat agagctatac
     2461 ttaaagaagt agctattttt tatactttgc caatgttaag tgcaactata tctagttatt
     2521 ttgcattgag attagcagga gatttattga tgaccgattt atttggtata tatatactaa
     2581 gtttagtgat atgtttgata atattcataa tatatggagc agtatctgtt aataagttta
     2641 agagtgtagt gtataaaaat tagttgatat attaataaag agaaaggaga ttcttgtaat
     2701 aagagtctcc ttgttgatta actaaataat ttgggtaata aattcatata aaatatgtta
     2761 tgatattaat aattcatagc atagtgaaat attaggataa tggagaatat acaaatgtat
     2821 agaattttat tagtagagga cgatattgat ttatcaaaag agattgcatt agcacttgaa
     2881 aaatggggat ttaaagtaga attaattgat gattttgagg tagtattaga tgaatttata
     2941 gataaaaaac cagatgtagt acttttagat gtaaatttac cattatataa tggattttac
     3001 tggtgcgaaa aaataagagc tatttcaaat gtaccattaa tatttttatc atcaagagat
     3061 tctgatatgg atttaataat gggtataaat aatggagcag atgattacat taccaaacca
     3121 ttttctatag aaatattggt taccaaaata aatggtataa taagaagagt ctataattat
     3181 agtgattcta atagtatttt atattgtgaa gatttgatgt ttgatgtggg gaaagggatt
     3241 ataaaacata agtataagga taaaagtata gagttgacta aaaacgaaat aaaaatttta
     3301 acattattat taaaaaataa gaatagggtt gtatcaaggg aaagtcttat gatgactcta
     3361 tgggataatg atgagtttgt gacagataat gcacttactg taaatatgaa tagattgaga
     3421 agtaaagtta aagaactagg ttttgatgat tttataaaaa ctaagaaggg gataggatat
     3481 ataatacaat gctagtaaaa tatttgaaag gtaggtctaa gtatattgct ctacctttag
     3541 ttgtagttag tataataaat atctacttat ttgctataga tatttttaaa aataaatatt
     3601 cagagttaat atatttagat tttttagtgc tgtttataat aatggtattt tttgttatag
     3661 attatataaa ttttagaaat agttacacaa atttatataa ttgtatagaa aatagtggtg
     3721 aaatagacag ttatttagtt gatggacaat cttttgaaga aaatctaata aaagatatta
     3781 tagaaaactt aaaaataaaa aataatagtg acatagagac ttataaacaa tcattaaaag
     3841 aattggatga gtacatagct aaatgggttc atgaaataaa aattccaata tcttctttaa
     3901 gtataattac agataggtta agtagcatag aagatagttt ggatattaag aatcaggttg
     3961 caaagataaa ttttttagta aactctattt tgtatagtag tagaagcaac actatgtttg
     4021 aagatgtatt tataaataaa tttaatttag aaaaattggt aaaaatgtct ataaaaaata
     4081 attcattttt gcttataaaa aataatgtag aagttagttt gaatgaatta gaaaatgatg
     4141 tttacacaga ttctaaatgc atgtcatatg ttttagacca aattattaat aatgctataa
     4201 agtattcaaa gaaagttggt aagattgaat ttaattcaaa aaagttagaa aatggagtgg
     4261 tattatctat aaaagatttt ggtataggta taaatgagga agatatatct agggtttttg
     4321 acaagggatt tacggggaaa aatggaagaa atcaattgta taagtctact ggaatgggga
     4381 tgtattttgt aaagaaaatg atagatagtt tagggcatga gattgaggta tgttcgaaaa
     4441 ttggttcata tacaattttt aatatctact tttatgatat atcggattat ttaagcctag
     4501 atagttaggt tattagacta taaaatctat catcatatac aaatcgacat atacaataag
     4561 aataaattta gttcttgaaa atagtgaaga tttaattagt gccttaaatg atgctctatc
     4621 tatattataa aataataatt attatatatg aaaaacatat cagataggta cctggtatgt
     4681 tttttatttg tagtaactat tttaatatgg tttttggaga gagaatatgt tttgtttgtg
     4741 aaaaaaaatt acatggtatt acattgacac tgaaaataaa ttaatgtaag atttagctaa
     4801 ggtaaaaaac gtgtataaga ttaactcaat taatttcata agagtaaatt atcgtaatta
     4861 aagatgaaat aagattaatt ctaaaattat taaacttaat accttgattt ggggaggaat
     4921 aactataaac ttaaaaggat gataactatg tatagtgtag gtttaatgtc aggaacttcc
     4981 ttagatggag ttgatgcagt actagctgaa attagtggga atggtagaaa tactaaagtt
     5041 aagcaaatag aatttataac tctagaaatt cctaaagata taaaagacga aataagaaag
     5101 tgctgtatag aagaggaatc gagtgttgat ttaatctgta gcttaaattt taagcttggc
     5161 tatttattta gtaaagctgt aaaatcagtg tgtcataaag caaactttca tatagcaaat
     5221 ttggatttta tcgcatctca tggtcaaaca atctttcata taccaagaag ttataataat
     5281 tttgtatctt caacgcttca aataggtgaa ccagcagtta tagcatatga aacaaataca
     5341 aaggtaatat ctaattttag agtaatggat atagcagcag gtggagaagg tgctccatta
     5401 gtaccatata gtgaattttt attatatagt gataacaata aaaatttagc acttcaaaat
     5461 attggtggaa taggaaatat tactataata cctaaatcat gtaacataga tgatgtattt
     5521 gcatttgata caggaccagg aaatatgata atagatggag tatgtcaaag attatttaat
     5581 agaaagtatg ataaaaatgg atactttgca tcaaaaggta aaattaatga agaaatgtta
     5641 aaagatttga tgagtcataa gtatcttagt caagcgccac ctaaaagcac tggaagagag
     5701 gtatttggac aagtttattt ggataacatg cttaacaaat ataaacatgt agataaatat
     5761 gatttgatag caacagtaac tatgtttacg gcaaaagcta tatattataa ttataaaaac
     5821 ttcatacttc caaaggttaa tgtagatact ttattaattg gaggtggagg agctcataat
     5881 ttaactttaa taggatatat aaaagagtta ttattagagg ttgcagtatt aactcaagat
     5941 gaatatgggt attcttctga tgcaaaggag gctttggcat ttgtaatctt gggcaatgaa
     6001 acattaaata attctttttc taatgtaata tctgcaacag gggctaaaaa taaagttatt
     6061 ttagggaata taacacctaa accttttgga ggtaaatgac atgtctaata ataaaaaaaa
     6121 gaaaagaaag aaaagaaaga aaaataataa ttataaaaat gcaaaaagta caaaaagtaa
     6181 taaaaatact aagaaacaaa tggagaaaaa tcaaagaaca gattataata ttccaactat
     6241 aagttttaaa agtggattaa tactaatagt ttcatctcta gtaagtactg tagtttttcc
     6301 tttgttatta tctatatttg gaatagataa tagatttgga gttgttatag gaaattcatt
     6361 aattacatct tttgcagttg catatacaag atactttgtt gaaactaaga gaggtttttc
     6421 tttgggattt tttagactgt atattttatt tgctttatca tttgcaataa taggttactt
     6481 ctggatatat aaaggaatac atatttaaaa aagtattgag actattttat gttaataata
     6541 agatagtctt ttttcttttt taagaaaaga tatgagtatt ttaagtagtg tgtaatttta
     6601 gacaaaagaa tgattagata aataaagcaa ttaaagcaag atataaattc atgaaataaa
     6661 attacaaata aaaatataaa atatgaaaaa atatatacaa acgtattcct gtaatcaaaa
     6721 atacatgcta taatgattga aaagaaataa aaaaacaggg ggaatcaata tgagagtatt
     6781 atttgtttgt tcatcaggga tgtcaagtgc tatagcagtt aatgcattac aaaaagaagg
     6841 agcaaaaaat ggaatagata tagatgtttt ggctgttggt actcaagagt ttgaggatga
     6901 agttaaaaat ggatgggata ttgctatggt agctccacaa gtcagacata gatttgacta
     6961 tttaaaagct tttgctgatg aggttagtgt accatgtgca ttaatacaag cacaagctta
     7021 tagtccatta ggaggaccaa agttattaaa acaagttcaa gagctgttga gtaaatagta
     7081 gttgcagtgt tgttaggtta gaagaaagtt gattttataa aaattttaat gataataaaa
     7141 ataaatatta attaaggaga gaaaagatgc tagataaatt tacagaattt ttagacacta
     7201 aattatcaac ttctatggcc agattggcag aacaacgtca cttaagagct gtaagagatg
     7261 gaatcgtagc ttcattacca cttataatag ttgggtcatt tttcttgata ttagcatttc
     7321 ctccattacc agattcatgg ggggtaactc agtatctaaa ggaaaatgca gttacaatat
     7381 tactaccata tagaatgaca ttttttatta tatcagtata ttctacattt ggcataggtt
     7441 atagtttggc aaagtcatat aagttagatg gattgacagg aggtttatta gctacagcta
     7501 cattcctttt gacaatagtt cctactgcaa tagagggtct tacccctgat ttattaaaga
     7561 tagttgaaaa gagtccagaa cttttagctt ggtataattc aattcctcaa ggatggcaaa
     7621 tgccaatggc taatcttggt ggaggttcaa tgtttgttgc tattgtaaca tctataatag
     7681 gtgttgagat ttatagattt actgacaaat ctaaattcaa gataaccatg ccagaagcag
     7741 ttccagcatc agtcacaagg tcttttgaag ctctgacacc agccgcatta ataataattt
     7801 taatggctat tgttacttat ttcctagggt ttgattggca tggcttcata gctaaattag
     7861 taactccatt agtttcagcc actgacacat taccatcagt aattatacta atatttttaa
     7921 taacgttttt ttggtcattt ggaatacatg gtgtatcaat aataggttca ttggcaagac
     7981 ctttatggat acaattatta gatgctaata cagcttcata tgctgcgggt caagcagttc
     8041 caggtattgc tgctgaaccc ttttttcaat ggtttatatg gattggaggt tctggagcta
     8101 caataggctt agctttactt cttgcattta gagcaaaatc agcttatgga aagtctttag
     8161 gaaaagcaac tgtaatacca gcaatattca atataaatga accaataata tttggagcac
     8221 caatagtatt aaacccaata ttaataatac catttgttgt ggcaccaata gtaatggcaa
     8281 cagtttcatg gtttgctatg agtgctaatt tagtatccag agtagtagtt actgctcctt
     8341 ggacattacc aggaccagta ggggcatatt tagccactgg tggagattgg agagcttcag
     8401 ttcttaactt attatgtata gctatatcaa taataattta ctatccattt gtcaagatgt
     8461 acgataagag gttacttcat gaagaaaaag ttgaagagca agaagcttat taattagaaa
     8521 ctatattgga aattaaaaac tatattagat tagtataaat ttcaaagtat aacagttatg
     8581 aaataagtaa cgaagatatt ttgttgattg tttataaaaa aagaaaaggt aaacttttct
     8641 ttttttataa atatatatga aacttttttt caccaaattt ttaaaaaagt ttcgtgtaat
     8701 tcgaaataaa tttaaacaca tataggatat aaggagatga catagatggg aagattagga
     8761 atatcaattt atccagaaaa aacaacatct aaggaaatat atgattatat agataaagct
     8821 gctgaaaatg gatttacacg tatattttca tgtttattat cagttaaaga tactaaagaa
     8881 aatataatta aaaaatttaa aagtataaat gaatacgcta agtttaaagg ttttgaggta
     8941 atacttgatg taaatcccag agtatttggt gaattagaaa taagctacaa agatttgact
     9001 ttttttaaaa agacgaaggc agacggaata aggcttgata tgggatttac aggttctgaa
     9061 gaggccttaa tgacttttaa tcctcaaaat cttaaaatag aaatcaatat gagtaataat
     9121 actaattata tagatacaat aatggactac caaccaaata aagaaaattt gacaggatgt
     9181 cataactttt atccacatag atatagtgga ttaaatttag aacactttat aaaatgtaca
     9241 gaaagattta ataagtatgg attaagaaca gcagtttttg taacaagtca aaatgaaacc
     9301 acatttggac catggcctgt aactgatggg cttccaacaa ttgagatgca cagaaatcta
     9361 cctatagata ttcaagtaaa gcatataata gctatgaata atataaatga tataataata
     9421 tcaaattgtt atccaacata tgaggagcta aaggtattag gttctatgag aaaagatatg
     9481 gttacatttg atgtagcaat agaggaaaat atacgagaag ttgagaagaa gatactcttt
     9541 gaagaaatac attttaatag aggggacata tctgacagtc tgataaggtc tactcaatct
     9601 cgtgttaaat ataaaggata caaatttgaa ctatttaata ctcctaaaat aataaaaaga
     9661 ggagatatag taattgaaag tagtgaatat ggtcattatg caggggagtt acaaatagct
     9721 ttgactgata tgaaaaacag tggtaaatcg aatgtagtag gatatataag aaaagaagaa
     9781 ttatttatat tagattatat taaaccatgg caaaagttta attttaaacc ctcaacaaaa
     9841 taatttatta tgaaaatatt aatttattat aaaaatataa ttatgaaagt tggaattaaa
     9901 atgtgtttaa aaatttaata ttaagaaaat gaggaaatga tatgttaata aaaactttag
     9961 aaaatttagt tacagaaggt agaaataaga atacattgca aatagataaa gaagatacat
    10021 taggcataat agaattaata aataacgaag ataaaactgt agcctatgct gttgaagaac
    10081 aaaaggagag tatagcaaaa gctgttaata taatagtaga tagaatgaaa cagggaggta
    10141 gattattcta tataggagca ggtaccagtg ggaggatagg aatactagat gccacagaat
    10201 gtcctccaac ttacggagtg gattttgagc ttgttcaagc tataatagct ggtggaaatc
    10261 aagcaatatt taaagcaata gaaggtgctg aagatgataa agaacttgga aagcaagata
    10321 taatagatag aggagttaca tctaaggatg ttatatgtgg tatagctgca tcaggaagga
    10381 ctccttatgt tataggtgca atggagtatg ctaaggaatt ggggtgtgca gtactatcaa
    10441 taactatgaa tccgaatagt gaaatgagta aaaaagcgga tttaccaata aacattatag
    10501 taggggcaga agtaataatg ggttcaacta gaatgaaaag tggtacagct caaaaaatgg
    10561 tatgtaatat gttaacaaca gcttctatgg ttaagatggg aaaagtatac tcaaacttga
    10621 tggtagatgt taagacatca aatgaaaagt tggttgaaag agcaaaaaga ataataatga
    10681 tagctacaaa tgtaaaatat gatgttgcag aaaaattctt agaagaagca gataatagcg
    10741 ttaaattggc tatatttatg ataaaaagtg gtttagataa ggatagtgca aagagtatcc
    10801 tagatagaca agaaggttat ataagtgaag ctttaaaatc aatagaaaaa ttgtaaaata
    10861 aataatagta ctgataacct ttcaatatta aaaaaacagc attggaaggt tatttttaga
    10921 tattaagtat tgaaagaatt attaaagata ttgtaggagg atagtatgta ttttttaggt
    10981 gtagatggtg gaggaacgaa aacaacattt acattagtag atgaagaatt aaatatagta
    11041 ggaaccataa ccaaaggaac ttgtcattat aatcaaatag gatttgataa tctaacgaaa
    11101 ttattaataa ctgggttgga agaagtttgt aaagatgcta agataaatgt agaagaaata
    11161 acgtatgcat ttgttggact tgctggatat ggaaaaataa aagaagtatt gtatgcatta
    11221 gaagtagcaa caaaaaatgc atatagtcat ataaattata ctctaggtaa tgatgtagaa
    11281 atagcattag caggttcttt aaatggagaa aaaggtataa atataattgc aggtactggt
    11341 tcaatagcac aggctttaga taaagatggg aatcttcaca gatgtggagg atggggctat
    11401 gtattaggag atgaaggttc agcgtattat ataggaatgg ctaccttaaa aatgttcaca
    11461 atgcagtctg atggaaggtg ttcaaaaaca aaattatatg atttaataaa aagacatctt
    11521 aatatagaga atgattatga cataataaaa tatgtaaatg atgaaataca aggagataga
    11581 atagaaatag caaagtttgc tacgatatgt ttaaaagcag ttattgaagg tgataatact
    11641 gctagtaaaa tatttgatga tgcagcttat gaattaagta ggcttataat aggattagag
    11701 catcattttg aacaaggtac aaagataaag gtatcttatt caggtggagt gtttaagtca
    11761 ggagatttaa tattagagcc acttaaaaaa tatttgaata aaacgagatt tggtatagtg
    11821 agaccagtgt tgactccaga tttaggagca tgtttacttg ctaagaagaa atattatagt
    11881 aaataggtag aggtgataga gtatgagttg cattttaaaa ttaaaacaga tatatgaaga
    11941 tttaacggaa gtagataaaa aaatagctga ttacatactt aataatacag aggctataag
    12001 taagctttct gtatcagaat tggcgtcaaa ctcaaaaaca agtacagcaa gtatagttag
    12061 gttttctaga aaaatgggat attcgggttt tggggattta aaaattgaga tagctaaaga
    12121 tttgatgggc aaagaaaatg aatacacata tgtagatgaa aactatgatt gtaatataga
    12181 tagtgttata aacaaaatta caaacaaaaa catagagact atcaaccaaa caaggttgtt
    12241 gaatgaaaaa gatataataa aagaagcagt tgaacagata ataaaggcta aaaatgtata
    12301 tatatttgga gtaggtggtt cggctttagt tgcactagat ttacagatga aacttttaag
    12361 gataaataaa caggcattta cctctttaga tagtcataca caacttatgg tatcttcaaa
    12421 tgtagataaa gaagatatag ctatagcaat atcttactct ggagaatcaa aagaagtaat
    12481 aaagtctata gaaaatgcaa aattaaaagg atgtaaggtc atttgtataa ctaaatatag
    12541 tgacaatcat cttagtaaga ttagtgattt aaagctagta gtaccaaata tagaaaaaag
    12601 gcttagagaa ggagctattt cttcaagaat agcaatgctt acgttgatag atataatata
    12661 tatttctata attcaagaaa acttaaatga ggcagaacag aaactacaag aaacaaaaag
    12721 gatacttgat tatttaaata ttaataatgc ttaaaactag gttatatatc aactaaacaa
    12781 ataagttagg ctataagcgt aagtcaataa gaaatagata aatatctaaa atattgactt
    12841 acttaataat aatatatagc atttttataa tacaaa
//
LOCUS       DACXMZ010000104        12724 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.105, whole genome shotgun sequence.
ACCESSION   DACXMZ010000104 DACXMZ010000000
VERSION     DACXMZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.105"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            117..305
                     /gene="rpmB"
                     /locus_tag="JD016_11310"
     CDS             117..305
                     /gene="rpmB"
                     /locus_tag="JD016_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAU5000221.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(465..617)
                     /locus_tag="JD016_11315"
     CDS             complement(465..617)
                     /locus_tag="JD016_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HAU5000222.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(715..1191)
                     /locus_tag="JD016_11320"
     CDS             complement(715..1191)
                     /locus_tag="JD016_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAU5000223.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1410..2171
                     /locus_tag="JD016_11325"
     CDS             1410..2171
                     /locus_tag="JD016_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAU5000224.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2431..4359)
                     /locus_tag="JD016_11330"
     CDS             complement(2431..4359)
                     /locus_tag="JD016_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAU5000225.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITISRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILDEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4593..5045
                     /locus_tag="JD016_11335"
     CDS             4593..5045
                     /locus_tag="JD016_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAU5000226.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYIINKMKEE"
     gene            5048..5356
                     /locus_tag="JD016_11340"
     CDS             5048..5356
                     /locus_tag="JD016_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAU5000227.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5470..6576
                     /locus_tag="JD016_11345"
     CDS             5470..6576
                     /locus_tag="JD016_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAU5000228.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6651..9329
                     /gene="mngB"
                     /locus_tag="JD016_11350"
     CDS             6651..9329
                     /gene="mngB"
                     /locus_tag="JD016_11350"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAU5000229.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEYSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9646..11967)
                     /locus_tag="JD016_11355"
     CDS             complement(9646..11967)
                     /locus_tag="JD016_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAU5000230.1"
                     /translation="MKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGAENPF
                     EQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTYDGSE
                     TYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLDTTKN
                     DASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYGVNQK
                     IINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGYGNTY
                     FDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLSRIPL
                     EILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLTYWFV
                     IFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLKEKPN
                     KTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLLDVTK
                     KISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVSHDLK
                     TPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGDVYLN
                     IVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLIINIS
                     KYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEGSGLG
                     LAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11954..12670)
                     /locus_tag="JD016_11360"
     CDS             complement(11954..12670)
                     /locus_tag="JD016_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000231.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         4754 a         1777 c         1659 g         4534 t
ORIGIN      
        1 attcttcaat atgtgtaagg caaatatata tttctattgc aaatttttgt tatttatgat
       61 aatatgaata tgtttttagt ctttaaatag ttatcatttt aaggaggtgt gcgtagatgg
      121 caaaagtatg tagcgtatgt ggtaaaggta aggtttctgg aaaccaagta tcacactcaa
      181 ataaacataa taaaagaaca tggtcagcta acttaagaag cgtaagagcg attatagatg
      241 gagctcctaa gagagtaaaa gtttgtacta gatgtttacg ttctggtaaa attgaaagag
      301 cttagtaacc acgtttaaaa gcctacttcc gtaagtaggc tttttaattt aatcattttt
      361 actctttctc ataaatttta ggattatact tgaaatccat tttggtaatt ttattgttac
      421 tactttcatc aatactcacc ccacacttta tagttaaaac tattttaaca aaaaaataat
      481 aatgctccac atccaataag tattaatgcg gtaagtgctg accaaatcca gccaggaagc
      541 aacattgaca gaattacagc cattcctgtt gctagacaaa gtattcctag caatacctta
      601 gacttgttat ttctcatgga acacctccac atagaacctg tatctataaa tattttcata
      661 ttcaagttta tgcgacagtt ttccatttta gaccaataaa cacattatgt aattttaatt
      721 tttcatacta gaattattac tgaactccga tatagattct acaatatcca aaattccttt
      781 ttttctactt aacctttcag gtccactata taaaacaact ggtataactt taggaagttt
      841 aaaattttta tcttctattt cttcaaggct ttttttcata ataaaacttt tccaaatttg
      901 ttctctatac gatttcaatc ttgggacaat ttcagtatcc atttctgttt gaaattctaa
      961 cactatataa tgtgatactt tgccactttt agaaaatacc ttataaatta tatcaacttc
     1021 ttttccttta tattcacata taaaagatgt ttctaactct atatttttaa gagttaattc
     1081 ttttgctatt gagacacttg taaaattttg aatcaaactt aaaaaacttt ctttatctga
     1141 atacattctt ctgtactctt ctcgtttttc attgcttctt tttaaattca tacagttcct
     1201 cctagactat agaccctcta ttttttagta aagatagtct ataaatttta ttttttaatt
     1261 ttcattagtg agtattgaca gttgtgtgta aaatatccag taaagatatt tttttatatt
     1321 tttattaaaa gatataaatt tttatatttt aatgtattga catttacgtt gcgtaaaagt
     1381 gtatatttaa tttataggag gtactactta tggaatatac agttcaaaaa ttaagtaaac
     1441 tagctggtat aagtactaga acacttagat attatgatga aataggtctt cttaagccac
     1501 ttaaaataaa ttcttcaggt tatcgtatat atggtcaaaa tgaagttaat aaacttcaac
     1561 aaatactttt ttatagagaa ttaggaatta acttagaaaa tataaaaaac attataaatt
     1621 caccaaactt cgatagttta agtgcgttaa aagaacacca taataaatta ctagccaaaa
     1681 gaaagcaaat tgatttacta atagaaaatg taactaaaac tatagctctg aaggagggaa
     1741 aatacactat gacagatata gaaaaatttg aaggttttaa agaaaagatg attgatgaaa
     1801 atgaaaaaaa ttatggaact gaaattagag agaaatatgg taaagatatt atagatacat
     1861 caaataaaaa acttaaaaat atgtctaagc aagattatga aaattggcaa aacctaaata
     1921 tcgaaataat tagtaaactt aaaaaagctt ttaaaactgg tgatgcctct agtgaactgg
     1981 ctcaggaagt tgcaaaatta catcataaat ggttgagtta cacttggaac acatactcta
     2041 aagaagctca ttcctctcta gcacagatgt atgtatatga tgaacgtttt acatcatatt
     2101 atgatagaga acaacctgga ttagctaagt ttttaaggga tgctatagta atatatacaa
     2161 gagataaata atataaaata ataaaggtgg tgtctcaaaa tgaacttttt agttcatgag
     2221 acactctttt ttgtatgaaa tcagatatat ttttatcatt ttatcaatga aaaacaggtt
     2281 tctctctcta ttttgagaca gaatctttat tgtataataa ttataatcaa tacaaaataa
     2341 taacaaatta tacaaaactt acttatacta tttatgctaa aaacaaaaaa tattttatta
     2401 aagtttttaa aacttatgta atataaaaat ctatactata gacttgatta tggaaagaaa
     2461 attatcaaaa tcattacaat tagatatact atctctaata tcattgtcat gaattatctt
     2521 taaaaggata tcataaatgg agtttatttt tatataatca tctttattta tagccaacat
     2581 catgattaat ttaacagtag aatcttcact ccaatatatt ggttcatcca atatggaaat
     2641 acaaatctta gttcttatag agcttaattc cataggatga ggtatagcta aaaaatcatc
     2701 tatattggta gaagataaac tttctctgta aaatacagat tcagcaaatg atggaaatac
     2761 aatttcactt tgagttaatt tgttgcacat caactttata agctcatctt tatctttgat
     2821 tttaggatta acaataaata aatttttatc aaagaaattg tccaaaagat tactataaat
     2881 atgggaatta ttagtgattg acttagatat attctctata tcttttttta atagttttaa
     2941 atccactaat acaataggaa tactatcatg atttaaagga attgtcgata ttattatatc
     3001 tacatctgac aattcactag ctaaaaattg attaaatgat agacattgaa gtatatttat
     3061 tttatcatga aaaactttgt ttaattgaac ctctaataac ctacttgaac cataccctga
     3121 accacatact aatactacgt ttttacactt aatattactt tgaaaaaatc tctctattcc
     3181 agcacctata tgcagggata catatccaat ttcatcttct gttaaagagt aaatactatt
     3241 tttaaaaact ttttctatag catttaaagt tatttcaaat gctaaaggat aattactctt
     3301 tattgtattt ataaggggat tcaccttatt taaattataa gatttagaat taagaattga
     3361 tttaaagtgc agtactaaat catcaaataa tacactatca tttctaaggt caaaagtata
     3421 ttgattgttg attacttcta aaatttcttc aacatactct ataattttag tatctaccct
     3481 attggaaacg ttatccaaaa gagaactttt agttataaaa tgattatata gataaactct
     3541 gtctgccttt gaaataatta tagtatattt actctcaata taattaaata tattttcaac
     3601 agttgtattc gattgaaaat ctgttaaaac aacatctaaa acattatcta agcagtaacc
     3661 atcaagtatt ctacttatag taatagctaa atggataata aagtttttta gattaaaatc
     3721 taggaaattg atattaaatt tattaatttc acttaacact accctttgaa gctctattaa
     3781 atctacatta ttaaataatt ctatttctct atctgtaatt ccaattatat agttttcaaa
     3841 attcctattt atcatattat tagaaataca tcttcgaata tctatttcct taccttcaac
     3901 tttaactcca taatatggct tagaaactaa tattaaatta tactttgaca aaataggctt
     3961 tattcttttt atgtcattta gtattgtaaa tttactcaca tacaagctat tagctaaatc
     4021 ttctagtttt atatatgtat tgttatagag aatatatttt aaaatatatt gatttctttc
     4081 tataggagaa tctggtattt cacttgaatc cataatatca tcagatatta atgctagata
     4141 ctgttggtat aatgctaaat taagaatatc tatataatat ccttctcctt tttttatttt
     4201 aataatagca ccatttttct ctaaagttcc atttatagaa gtaatatcat ttcttatagt
     4261 tctctcactc acttttaaat gattacttaa agttttacaa gtaattttat catcactttc
     4321 atttaataat ttaaacaacg tgagcaatct cttgtgcata aatatccccc cttatagaat
     4381 ttcttctaaa ttatactatt tacactcata taatacaatt actctaataa aattgtatta
     4441 tcataattta tttccacttc tatatggaaa ttagttatta tcataatata aatttccatg
     4501 tcgaagtgga aattagttat atttaattta attaggataa tgattacaat ataaatataa
     4561 gttaagacat tatgaatgga gtggaaaaat atatggtact acaagaaaaa tatatattta
     4621 tagataaaga cttaaaaagc aaagaagaag tattaaattt tattgcagat agtgcttata
     4681 tactaggtat tacaactaat aaagaggctc ttttaaaaga ccttctaaaa agagaaaatg
     4741 aatttccaac aggaataaaa gatggatttg cgatacctca tacaaaaagt gtaaatgtaa
     4801 aggaagcttc catactctat attaaaacaa ctaacgagtt agagtggaaa actttagatg
     4861 atagtaaggt tagatatgta tttagtttgt tagccccaca taaaaatgaa ggaaatatac
     4921 atttgaaaac tctatctaaa ttagccacat gtttaatgga ggatgatttt acaagtgaag
     4981 ttaaaaatgc tagtgatgca agtcatttag taaaatatat aattaataaa atgaaggagg
     5041 aataattatg aaaatagtag caataacatc atgtatagca ggattagcac acacacctat
     5101 ggcagcaaaa gctttagaac aagctgcatc aaaaatgggt catgaaatca aagtagaaca
     5161 acaaggtgct atgggacgag taaatactat aacaaaagaa gaagcacaag atgcggactt
     5221 tatacttata gcatctgacc aaactatatc tggaatggaa agatttgatg gtaaaaaagt
     5281 gataaaagtc aaaataggta tagcgttaaa aaaaccagaa gctgtcttaa caaagtgtat
     5341 agaagctatt tcttaataaa aaaatctcac tctaatatag aatgagaaaa atcaaaaatt
     5401 aaaaaattgt ctaatgtaat tataacatat tatcaaaaaa tataacaaac ttatatagga
     5461 ggattaaaaa tgagtaaaaa aaataaatct cttttaagtg aacttaaggg acacttaatg
     5521 acaggtatct catatatgtt gccacttgta ataggctcat ctttagttgt agctatccca
     5581 aagcttacag ctttagcaat gggtataaca agtttagatg cttataaaaa tgtatctggt
     5641 ttttatcata ttctttactt aatggaacaa gttggttgga ctggcatagg tctattaaat
     5701 acggtattag caggatttat agcattttca ataggtgaga aaccagctat gggtgcaggt
     5761 tttattggtg ggctcatagc aagtaatact aatgcaggat tcttaggagc agtaatagca
     5821 ggtttctttg ctgggtatgt ttgtaagttt ttaaagaaaa aaattaaaat atctgggtca
     5881 gcatcaggaa tgatgccact tataataatg ccacttataa cagttggatt aactggtttt
     5941 ttaatgagtg ttgtattagc aggaccttta ggaaacataa atacttcatt aaatgcttgg
     6001 gtagctgaaa tgtgtcaaaa tggaacaaat tcggtggtat tggctttaat tttaggcgca
     6061 atgataggat tcgatttagg tggaccagta aataaagcag cttggatggc aggaaatgca
     6121 ttattgttag agggaatcta tctaccagcg atattggtaa actgcgcaat agttatacct
     6181 ccactaggat atggtttagc tacattcata agaaaatcaa agttttccaa tgcattagct
     6241 gaaacaggaa aaggcaatat agttatgggt ataattggaa taacagaagg tgcaattcct
     6301 ttcactttaa caaatccatt aaaattagtt cctgttaata tgataggatg cgctttgggt
     6361 gtaggattat cagctttgtt aggtgtacat gctataatgc ctccagttgg tggattatat
     6421 ggatttatat caataggttc tggatgggct tacttagtag gtgcaatatt tggagcttta
     6481 gtaatagcaa tattatcaac aactttagta gacttcaatg aagatgaaac tacggaaaat
     6541 acagaatttg atgaaataga gttagatata atgtaaatta aaaacttaat tttagataat
     6601 tgagaattga gtgtactctc aattctcaat taaattttgg gaggattttt atgaaaaaga
     6661 caaaagtaca tgtagttcca cacagtcatt gggatagaga atggtatttt acaacatcaa
     6721 ggtcaaaaat atatttaatg aaagattttc aagatatatt agacatattg gaagaaaaag
     6781 aagactttaa atactttact atggatgcac aagcttctct cttagatgat tatttaaaat
     6841 ggagacctca ggataaatat agaataagag atttagttca aagaagaaga ttagttattg
     6901 gaccttggta tactcaaaca gaccaattgg ttatatcagg agaatctata attagaaacc
     6961 tttactatgg aatagataga tgcaatgaat ttggtgaacc tatgagggtt ggatatgtac
     7021 cagattcttt tgggcaatca gctcaaatgc cacaaatata cagaggtttt ggaatagata
     7081 gttcattatt ttggcgtgga gtttctgatg atatggtaga tacaacagag tttatatgga
     7141 aaggttctga tggttcaaaa gtattagcag ttcaaatacc atttggttat tactatggag
     7201 gtaatatacc agaagatgat tcagatttaa aagaatattt aaaagatata attggaaaac
     7261 ttaaggttaa agcttctact aataacgtat attttccaaa tggatttgac caagcaccaa
     7321 taagaaaaaa ccttcctgat atattaaaaa aagctaatga aatagataat gaaaatgaat
     7381 acgaaataag tgatatagaa aactatatag attcagtaag aaatgaaaga aatgaattta
     7441 cagagcttaa aggtgaattt ttaaatgcta aacatatgag aatacacaaa tccatattct
     7501 cctcaaggtc agaccttaaa ataatgaata acaaattaga aaattatatt acaaatgtac
     7561 ttgaaccatt attaacatta agttattcac ttggaaatga ataccctcat ctagttataa
     7621 aagatatatg gaaacttatg tttgaaaatg cagctcatga cagcataggt tcttgtaata
     7681 gtgacacaac aaatgaggat gtatacacaa gatacaaaca agccagagat ttgagtataa
     7741 atttattaga tttgcatatg agacttataa gtactcaaat caaaaataat aatgaagaaa
     7801 taacattaac agtattcaat ggattcccag aagtcagaaa tgaagttgta gaatttgata
     7861 cttatattcc aggtcaagat tttgtgataa agaataaaaa tggagaaatt cttaattata
     7921 taataaaaga aaaagaagat attacaaatt atatattaac tcaaacaata agattaaacc
     7981 caagtaaaaa aatatatata cccaaaactg tatatagagc aaaaattgca ctttcagtaa
     8041 aagatatacc accaatggga tatactcaac tagtttttga attggatact aaaaataaat
     8101 gtgaaataga aacaagtaat tgcaaagaaa tagaaaacaa atactacaaa ataacagcca
     8161 ataaaaatgg ttcattagaa atattagata aagaatctaa taagacttat aaaaaccaag
     8221 ctgtaattga agataatgga gatgatggag attcatacaa ctattctcct ccaagaaaag
     8281 atatttatgt ttcatcttta gattctgtat cttcagtaga agtgctaaaa tcaaacattc
     8341 aagaagaaat gatacttaaa tattcattga ccataccagc ttcactggaa gaaagggcta
     8401 taggtaaagt ttctgtgaaa atgccagtaa caatgaaaat taccttagaa cctaaagaac
     8461 aaataattaa gtttaatgtt aatataaaaa ataaccaagc actatctcat agagtaagag
     8521 ttttatttaa tacagaaata gcatctaagt tttctatagc agatcaacaa tttggaataa
     8581 tacaaagacc aacagtatta gaaaaagacc ttgcattgtg gaaaagagac aattactcat
     8641 ggcaagaaaa acctataact atcgaaccaa tgcaaagttt tgttacttta gaggatggac
     8701 aaagaggaat agcattaata actggttgtg taagagaata tcaaatagtt ggtgataatt
     8761 tagatacaat agcattaaca ctattcaggt catttggata tatgggaaga gaaaatttac
     8821 tttatagacc aggaagagct tctggagaaa aaataatagc aacaccagat gcacaattac
     8881 ttaaagagtt aaactttgac tttggtcttt acatatataa caataaattt gatgaagcaa
     8941 atgtagctaa tacagctaag agattcttaa cacctataca aatatatgaa tatgctgatt
     9001 ttttaaatgg tagacttata tttgcattca atgacgaaaa acaaatatat gaaaatgaat
     9061 atagtttgat gaatgtaaat aattcaaatt tcacagtaag tgcaataaag aaagaagaaa
     9121 aaggagatgg aattgtagtt agaatcttca atggtatgaa aaatgaggat gctaagggaa
     9181 gtcttaacat caataaaaat gtcaatgatg cttatagtgc aatgctcgac gaaatctatg
     9241 ataatactag caagcttaaa gtaaatacta aaactgtaga ggtaaattct ttatcacatt
     9301 gtaaagtaca aacaatagta attaaataat ataaaatagc ttatacaaga tttcaaaata
     9361 aattgctagt actatgttaa tagtaaagcc agatattatg ctatttataa gctctgtaac
     9421 actaaataac attttgtaaa ataaaaaggt gaaccttgta tattaggatt cacctttttt
     9481 aaataaatca acattctgtt ttaaaataac atttataagc tgttttgaaa taatgcttac
     9541 aaattatttt aaaacaatac ctataaattt ttatttaaag aatacacata aatatttcca
     9601 gactctttta tcctatagac tttataatta ttaaagaagt ctaatctaat ttttgttaaa
     9661 agtaattgta actttgaata aatcaccatc cacacttata ttcatcttac caccctgtaa
     9721 ttctataaaa ctttttgcta ttgctaaacc aagaccagaa ccttctgtgt tacgtgactt
     9781 atctcctctt acaaatctct ccattatatc atctacacta aaatcaatct cagatgcaga
     9841 catattttta agcataatat ttactttatt atctgtttct aaaatatcaa tatacactct
     9901 tgaatttggc attgcatatt tgcttatatt tattataaga ttttcaaata ctctaaatgt
     9961 acgctgacta tctaatggta atattatttt ttcattagaa aagttattct ttactattag
    10021 agatgaatct gcaagtttgt catctaattc taaaagtgtc tgcttcatta gagatacaat
    10081 atccacattt actatattca aataaacatc tccactattt gccttgctta cttcaaacaa
    10141 atcttctatt agatgttgca atctttgtga ctttctgtct aatgtatcaa tatacaactt
    10201 acgattttca tctgttatac tttcatcttt taataaatct atataagtga ttatagatgt
    10261 tagaggtgtt tttaagtcat gtgatacatt tgatataagc tctgttttca tcttttgact
    10321 cttaacctct tcgtcaacag cttttttaaa tccagtttga atatttccta attcatcttt
    10381 tataggatta aaaaatccta aatctttttc catatttaca tctaagtttc catctgaaat
    10441 tttctttgta acatctaaaa gttgattata ttcatatatt gtctttttag agtaatctga
    10501 cagtatctta aataatacta ctgaatatat taatgcgagt attattccaa agaaccacat
    10561 actacacatt ataactataa tcaataaatt tattcccaaa attgtaacta tagttttatt
    10621 tggcttttct tttagattta ttgttctaat ttgattaata actgtttttc ctgctttttt
    10681 tagccataca aaaaacattc ctgtcacagt atgttttgta aaatactctc ttattccaac
    10741 atttaaaatg tgttttaaaa gtattataaa tacaaatatt attgagaata ttacaaacca
    10801 ataagttaaa ttcattaaac ttaccaatat actagccatt ttagcactta tgtcattgtt
    10861 tataaggtac tctacataat tcccactcaa ggtattactt attataacag taggatataa
    10921 atctacaagc ccctgtataa tagcaagagc aataaagagt atctctaacg gtattcttga
    10981 taatgtatca aatcctagca actctttaga ttttctatat ggaaatgcta aagctacaat
    11041 aacaactata gctaatataa tttttaaaat cattccagaa atcttataat aagaattatt
    11101 ttctacattg cttatatatg aaattatacc atcatcaaaa taagtatttc cataaccata
    11161 ctctacacta tcacctttac ttattacttt aggtacagca tatactaatg ttatattttt
    11221 tattggagat aacttatatg aaattgaatt tcctcctaaa cttgtatcca ttaaatcttc
    11281 tcttgcttta gataacaaca tttgattaat aattttttga tttactccat aagaatccac
    11341 aatatttact tttccatctt tatcaaaatt cattacaacg tagaatctat agttttcttt
    11401 tatatctgta atctgctttt gtctttcctc atcattcatt tctgtatttt ttgactgatt
    11461 ttcatcagaa ttttcttttg aagcatcatt ttttgttgta tcaagtactc ctaattcttt
    11521 attatcatta aaaaattcaa aattgctttc tttatcaact aaatagtact ttaaattctt
    11581 tcctgacgct ttaatgaaat gctcccaatt ttcaaagttt tcattaaaat ctgcattaat
    11641 actatcatct gtatagtaag tttctgaacc atcataagta tctttttctt tacttacatt
    11701 ttttatatag ttcatagttt cttcattgat aaatagcttt gatggttgta tcatcttacc
    11761 ttcttgttct tccttcatat tatgatataa aacataactt cctttatata tactatctac
    11821 aaagctttcc tgttcaaatg gattctctgc tccaccttta gcacttgctt gtatagtaga
    11881 atattgacta atcataccta atgatgctga taacataagt ataaaaacta taatagtaac
    11941 tatatattta tctttaaatt ttttcaattt tgtatccaac accccacacc acctttaaat
    12001 atttaggatt ttttgggtca atttctatct tttcccttat attcctcaca tgtaccataa
    12061 ctgtatctgt atttacagca ttctcattcc aaactctttc atatatttca tcagcagaaa
    12121 aaactcttcc tgcatttctc ataagtaaac ttagtatttt aaattctata ggagttgttt
    12181 ttatatactt tccatcaata ctaacttctt ttgtattttc atttaattct aatccgccta
    12241 ttgcaaacac actatcgtca ctttctacct tttgctcttt atttccaacc atgtttagat
    12301 acttagtgta ccttctaagt tgtgaattaa ctctagccaa tagttctaat ggcttaaaag
    12361 gttttgttac ataatcgtct gctcctatat taagccccat tattttgtca acttcctctg
    12421 attttgcaga tagcattatt actggaaaat tatagttttc tctaagcttc attgtaagtg
    12481 ttataccatc cattttaggc atcattatat caactatagc taaatgtatt tcttggtttt
    12541 ctataacctt aagaccttct aatccattgt atcccttaaa aacattatat ccttgattta
    12601 atagatatat ttctatagca tctgctatct ccttttcatc ttctaccacc aaaatattat
    12661 atgaattcat tctaatctcc tttatattaa tttataaatt ttctttacat atatgattat
    12721 acaa
//
LOCUS       DACXMZ010000105        12455 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.106, whole genome shotgun sequence.
ACCESSION   DACXMZ010000105 DACXMZ010000000
VERSION     DACXMZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.106"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(83..3511)
                     /gene="uvrA"
                     /locus_tag="JD016_11365"
     CDS             complement(83..3511)
                     /gene="uvrA"
                     /locus_tag="JD016_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HAU5000232.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDIGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKTGSQNKTIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            3830..6064
                     /locus_tag="JD016_11370"
     CDS             3830..6064
                     /locus_tag="JD016_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAU5000233.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGEGTIEIINSKGKPLFIGKNTELIKDLSHKYKDSTDEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            6320..8350
                     /locus_tag="JD016_11375"
     CDS             6320..8350
                     /locus_tag="JD016_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU5000234.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAKKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            8347..8799
                     /locus_tag="JD016_11380"
     CDS             8347..8799
                     /locus_tag="JD016_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAU5000235.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            8801..9856
                     /locus_tag="JD016_11385"
     CDS             8801..9856
                     /locus_tag="JD016_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAU5000236.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            9923..10231
                     /locus_tag="JD016_11390"
     CDS             9923..10231
                     /locus_tag="JD016_11390"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAU5000237.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            10335..11600
                     /locus_tag="JD016_11395"
     CDS             10335..11600
                     /locus_tag="JD016_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphate kinase"
                     /protein_id="HAU5000238.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            11688..12401
                     /locus_tag="JD016_11400"
     CDS             11688..12401
                     /locus_tag="JD016_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAU5000239.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSKKC"
BASE COUNT         4561 a         1484 c         2077 g         4333 t
ORIGIN      
        1 ttgtacagct attataataa ttcttctata atataagaca ttctataatt ttatatttaa
       61 gttcatgtgt atttaattgt ttttatataa aatgtccagt aatactattt ggatttattt
      121 ttatttcatc aggagttcca gaagcaacaa tttcccctcc atttattcca ctgtctggac
      181 ctatatcgat aatccaatca gaagcacgta taacctctgt attgtgttca ataattaaaa
      241 gtgaatgctg ttcatttaca agttttctaa aagtaattaa caaattttct atatctttaa
      301 aatgaagtcc agtagtaggc tcatccaata tgtacagcat tcgtttttta gtttttgtcc
      361 ctaattcttt tgctaatttt atcctttgag attctcctcc acttaatcct aatgtatttt
      421 gtccaagctt tagatatcca agtcccactt catctaataa ggagagtttt ctatatatat
      481 cagagcaatc gctaaaaaat gtttttgctt ctgctacttc catgtcaagt atatctgcaa
      541 ttgtctttcc cttgtattgt atagacagta tttctttttg atatctctta ccttgacatt
      601 cactacaagt tacccattgg tctgccatat attgcagaat aatttttgtt ttacctaatc
      661 cttgacagtt tgggcattgc ccctttttgc tattaaagct aaaatagctt tcatccaata
      721 atttttgttt tgcttgtgga gtttcagcaa atagagaacg aatttcatca aaaatattga
      781 tatatgtagc aggtgtagaa cggctagatt ttccaatagg tgtttggtcc attaagataa
      841 aatcatcaat agcatcaaat ccagtaaatt cagtataatt tttatctggg atagattttt
      901 gtttcaattt ttcttctaga gcaggaagta aactgtgaaa tacaagagag ctcttaccag
      961 aaccactaac acctgtaata gagcacatac agtttaaggg aatttctaca tcaatatttt
     1021 ttaagttatt tgcatggcat ccttttaagc ttaaccattt acttggtata gttttatttt
     1081 gtgaacctgt tttattgtat gtacttaaat atttacctgt tatagagttt ggatttttta
     1141 taatttcttg ggttgtacct tctgcaataa tgaacccccc ttttgtacca gcacttgggc
     1201 ctatatcaat aatataatca gcagacagta ttgtatcttt atcgtgttcc acaactataa
     1261 cagtattacc cttatctctt agctcttcta tcattttgat gattaaatta tggtctcttg
     1321 ggtgtaaacc aatagatggt tcatctaaaa tgtaggttaa gcctactaat tcacttccta
     1381 gctgacttga taatcgtact ctttgcagtt ctcctccaga taaagttgga gctgttctat
     1441 ctgttgatat atagctaaga cctaccttta atagataact aactcgaatt tttaattctg
     1501 ttaagatatc atttaccaaa gagagctcat ttttttgaag ttgatttggt aattcacaca
     1561 accaattcca taactgttct atcgtcattt ttgttagttc agggaagcga taacctttga
     1621 ttgtagtgaa tctagcctca atgcacagca attcgccacc acatgtattg caaggaattt
     1681 tgttcatata ttgatgcaag gtattatttt cagaactaga ttctctaaaa agtctttgaa
     1741 tgtggttgat tgctcctgaa gcaggtcttc taatctttga ttctcgtccc tttgattcaa
     1801 agctaaattc atatatctta tcgtctgtac catataagat tgcatctata aaatctcttg
     1861 gtaattcatt ccaaggtata tctaggtcta tatttttatc ttttgctatt gcatataatt
     1921 cacctaccat ccaattacca tttggctttt tccccctcaa ttttccaaaa tagtaagtag
     1981 ctccatctaa tattgaaata gttgggtctt ttacaattaa ttgaggatta acttgatagg
     2041 taaaacctaa tccattacaa tcgtgacaag ctccagtatg ggagttagca ttgaaggttg
     2101 aagtatttat cttagaaaaa atagtttgac aatgcggaca ataatttttt gtattattct
     2161 ttttttctaa tggaatttta caatatggac agtaactttt tccgattgat acaaataaca
     2221 ttcttaaata atcccaaatg cttgtaagag taccaacagt agagcgagga ttttggcttc
     2281 cttttttctg ttcgatagct atagtagggg ttaatccctg aagtaaatca aattccggtt
     2341 tttgaagctg ttctgaaagt tttgctccat tacttagatt ttcaaaataa cgtctttttc
     2401 cttcttcata aatcgtatcg aaagctaaag aagattttcc actaccactt acacctgtaa
     2461 taacagttat ttgatttttt ggaattgtaa cctcaacatc ctttaaattg ttttgccttc
     2521 ctccttttaa atagatagag cttttattat gacaaagtga atttttaatt gatttattta
     2581 ttgattttgt aaatttgaat ttcttttcta gttcaagagg attgcctaat tcttttattt
     2641 ccgaaatgcc taatattttt aactgagaaa agattgattt caaataatct tcaggcaatt
     2701 tttctattaa caaattaagg tcttctattt ttatatttcc gcttgataat atttctgcca
     2761 tttcatctaa taaatttttt tctgctcttg ttaattgatt gtattttttg gaacttaatt
     2821 ctgtccataa atcccatgga agcaattcta aaccaatcat attattaaag tcatgtagaa
     2881 gattaccttt tacagaatgc caacctttcc aatttttatt gtatccaaag tcatctggat
     2941 gagcatgtcc ttgcctacat cttatccagg cttctgaacc agttaaaaaa ccatctgttt
     3001 ttccgacatc atgagtgtca aaattaattt gaaaataatt tttttgtatg tcatctattt
     3061 gtgcatctat tcttatccat ctctttgtta gagtatcata gtattctaca agccagtggt
     3121 cttcataagt aagctcactt tcaaaataat tagcaaaacc tgctctcatt ctagcctcaa
     3181 aacctcgata tcttaggagc gatacaagaa gtaaagaata atctctgcac attccaacaa
     3241 gcctttgttt tgggtttctt ggaatagtta gtgggttaga atttatttca aatattcttt
     3301 ttaacatttg aggaattgta cgaagcaatt cctcatcaga ttgttgctta ttaaaggata
     3361 taccatataa ttttccttgg tctccatgaa ttactaggct ttgaaccact tttaataact
     3421 caattggagt ctttggtaaa tcttgatatt tattttcaaa ttttttagga tttgtgatga
     3481 tgctttgatt tttatagtta tttattttca tttatatttc acctctctaa atggatgata
     3541 taccctgaca tggtgtcata tgcaagggca atttagattt tttgaccaaa atttaagaat
     3601 aaataaaaaa tgatacagtg ataaattgta atacaaatat agtttcaaat tgcatataat
     3661 ataattattt aataaaacca ttatctatgg aatgttaatg aaatgttaag atggcattct
     3721 tgaatattaa gaaatagaat actataatat agtgtgaagg atttttagta aaaaaatttg
     3781 taagtgttaa tatacattaa aaaataggtt tataatttgg aggaaaaaaa tgaaattatc
     3841 aaggtataag aaaaatagtt tagttctgtc tataatttta tgttcactta ttatttcaat
     3901 ccttgtaatt actgttttta ataccgtaaa actacacaat atatctaata aacaatctaa
     3961 aaactattta aatgatgtat ctacacagat tgtaatgaat gttgattcta aaatcaaatt
     4021 tattttatcc gacttaagaa taatggcaga ttttattaag caatatgaag gtgatagtcg
     4081 atatgagtat ttatctaaaa gaaagactag ttataagtat tatgatgttg gaataataga
     4141 tttagaaggt aaagctgagt ttttaagtgg aaaaaagttt gacttaaaga atactgcttc
     4201 ctataaaaaa ggaattgaag gtaaagaata ctcagaagta atagagtcaa tgggttttgt
     4261 tctttatagt gtacctatat ttaatgataa tcatgaagta tctagtattt taataggtgt
     4321 atccaataaa gaaagtatga atgagattct taatatagat aactttaatg gagaaggaac
     4381 tattgaaatt attaatagta agggaaaacc tcttttcata ggaaaaaata ctgaattaat
     4441 aaaagacctt agccataaat ataaggattc tacagatgaa ccttgggcac aaaagatgct
     4501 taatgatttt aaaaatgata aatcagggaa tattacaata acctcatcaa aaggtgtaca
     4561 atgcttattg acttatcatc caattacaag aggtttaaat gattggcatt ttcttctcat
     4621 agtacctgaa gatgctgtac ttggtgaact taataagtta aattctttta caataacaat
     4681 gacatttata attacatgta ttattggaat tataacatta attttatata ttataagaag
     4741 aaaatatgta aatcaaatag aaaatattgc atatttagat agtataacaa atggcataaa
     4801 ttcaactaag tttagtatgc tagtcaaacc tttaatttca gaatcacctg atagtactta
     4861 tatgatgata gcaatgaata taaaagattt taagctaata aatgattgtt tcggtagtga
     4921 aaagggaaat atgacactaa aacatcttta caatattttg tataaaaaca ttgatgaaaa
     4981 tgaagaattt gtgtgccgtc atgatgcaga ccttttctat ttgcttatta agaatagacc
     5041 agtgttagaa gccctggagt tattatataa aatagaagct gacacaaatt attttaataa
     5101 aaatagggaa aatccatact ttttaagact atcagtaggt atttacacaa ttgaaaatca
     5161 taatgaagat ttaattacaa tacaagacca tgcaaataca gcaagaaaaa gctttaataa
     5221 aactcatcaa agtgattttt ctttttatag tgatatagaa agaaaacgat taatatctga
     5281 aaaggaaatt tcaaatttaa tggaaaaagc ccttgaaaat aaagaatttt ttatgtgctt
     5341 acaacccaaa attgatataa aaagtggaaa aattagtgga gcagagtctc ttgtgcgttg
     5401 gaaaaattca gaaaaaggca taatttatcc aagtgatttt attcctcttt tcgaaaaaag
     5461 tggatttata tgtaagttag atttatatat actagaagaa acatgcaaat tgatttctaa
     5521 gtggataaaa gaaggtaaag aagtgttgag aatttctgtt aatgtatcac gtcaacatct
     5581 gaatgataaa ttgtttttag aaaaatataa gcatatatgc aataaataca atgtaccaac
     5641 atgtctgata gatttagaac ttaccgaatc tattttctta gaaaatccag aagctataga
     5701 tataatagag gatattcatt ctaatggatt taagtgttct atagacgatt ttggatttgg
     5761 gtactcatca ttaggaatat taaaggattt taaagttgat ataattaagt tagaccgttc
     5821 attctttgtt agcaaaaata atatagaccg tggtaaggta gttattaagt ctattattga
     5881 acttagcaaa aggctaggta tgaaagtaac tgctgaagga attgaagaat tagaacaagt
     5941 tgagtttcta aaaaatgtgg gatgtgatta tattcaaggt tatgtttttt caaaaccatt
     6001 attaatttct gactttgaaa attttgcata tgaaaataat aaaattaaaa atatccatta
     6061 ttaaatgtac aagataaaaa atttaggcaa gttatataat taaaaatata acttgtcttt
     6121 tttatttata tatttaataa aaatatattt gcagattttg taaaaataat tttcaaatgt
     6181 tgtaagttgt ggctaaagct ttattgtatt attctgaata tagaaagata gttactgtct
     6241 tagataaaaa ctgaaacata cataataata taaaatttgc tatattaata actctaatta
     6301 ttttggaagg agggaaaata tgataagtgg gagaatgtta ctcatcatga attttctaaa
     6361 aggaaaaaat aagacaagtt acaaggatat tagcaaacaa cttagtatag aagaaagaaa
     6421 agttagatat gatataaata atttaaatat tgttttgcgt tcattaaaca agtcagtaat
     6481 acaaaagatg aacaaaggtg tgttaattat tcctgatgac ttccaagtag tatgtataaa
     6541 taataacgaa tatgtatttt ccttaactga gcgagtatct attatgaaaa ttatatcaat
     6601 gtttaaaata gataatctaa acttagaaaa attaagtaaa gaatttcagg tttctagaag
     6661 ttcaattaaa aatgacctta atgttttgtc aagtgaatta gaaagaaacc aaataactat
     6721 tacttatgat aaatctttta aaataagtgg agaagaagac attatatata agcaaagatt
     6781 gaacatatta aaatcgtatt cttatctttt tggtaaggaa aaatctgatt taagtgtatt
     6841 tgagaagtat ttagtgaatg taattgaaaa aatattcaaa gggatttatt tgattgatat
     6901 atataattgg tctatgaatt tattattgca aatgggatgg actttaaatg atgaatcttt
     6961 tacatggtat gtgtcaaaca ttttcctttt tacatggtat atacattcag atgaaaaaca
     7021 tcctttagaa tctgcttcat taagtgagcc gtgttttgaa aataagtttg accaagaatt
     7081 tgaaacaatt attcaaaaaa agttgactaa ggaacaaatt tttctgttaa agagttttgt
     7141 attttttaca aataaatatg caagtttaaa cgaggatatg gatttgatta gtacagaaat
     7201 tatagttcaa gacctaattt acaatatgtc agaaatatta gagattagct ttaaagaaga
     7261 tatgattctt tataaaggat tactcaatca tattgcccca ttgatagaga gaatcaagca
     7321 gaatatacag atttatgagg aattacttca tgtaatacca gataaatatc aatatgtact
     7381 tgaagctact aaggtttcta ttcaaaaaaa cagagtttta agtcaaataa agaatgaaaa
     7441 tgaaacaata cttttggcaa tacatttctt aggaagtata catagaaatg agaggaataa
     7501 ctacaaaaat atattgttag tttgtggatt tggatatggt gtaattgcta tgttaaagga
     7561 tagtttagta aatgattacc agataaatat agtagagagt attccatcat ataagttaca
     7621 agattataaa gaatgggaaa gtattgactt cattataaca acatccaaga tagtaataga
     7681 cttgcctaaa acaattattg agataaatcc atttttacaa gaagaggatt atttgaagtt
     7741 tgaagaaaaa gggataaaaa gaaagaacgt cttaactaat tatatgtcaa taaaaaaaag
     7801 gttggacttt ttagaagaag atgccaaaaa aagagtatta agtattatat gtcaagaact
     7861 tggatacagt gatgagagaa tattatttag acaacttaat ttaagtgatt taatagggat
     7921 tgataccata aggataattg atgaagatat gaaatgggaa gatgctgtaa aaattagttc
     7981 aaacatactt gctaattgtg attttgtatc agcacaatat gctgagaata ttatagatat
     8041 tttaaaaaat gtgggttttt atgctgtaaa agataacgaa tttgctttac tacatggcaa
     8101 tgatagttca ttagtcaaag taagttcgat aagtttatta atatgtaaag aagcagttag
     8161 ttttggagat aaaaaggtta aaatcatatt ttgcctagca agcagagata aaaaagagca
     8221 aattcctgca ataattaatc taactaaaat ggtttataag actaatttta ttgctatgtt
     8281 agaaagtgca aagacaaaag aagaagcaga agttttaatt cataaatatg agaaggaggt
     8341 cactgtatga ataaattagt taatgtaaaa tgttcattta aaatacatgt taattcaaaa
     8401 gaagaagcca ttatatcgtt agttgatgta gtgtcaaaag aaggatattt aatagataaa
     8461 aatcaatttt taaaagatgt tttaaagaga gaagaaacat tgtcgactta tattggacat
     8521 ggtataggtc ttcctcatag tcaaagtgta ggtgttaaaa actcttgtat tacaatagga
     8581 aagcttgata ctcctattga atggacagaa gaaggagaaa aagtggattt aatatttttg
     8641 atatcagtaa caaaagacaa tgaaaataat cttcatttaa agattttatc aaaattagca
     8701 cgtttattga tgcatgaaag ttttagaaat caaattagat gttcagatga acaaactgtt
     8761 tataatctta taaaagaaaa aatagaggag gaagattaaa atgagaattt taaagttttt
     8821 gcaaaaacat cttatgacag caacatctta tatgattcca tttgtggttg caggaggaat
     8881 attatttgct ttatcagtaa ctttaagtgg acaagctgct gttcctgaaa caggctggtt
     8941 ggctaaactg aatcaaattg gagctgctgg cttggcttta ttcattccta tattaggtgg
     9001 atatatagca tttagtatgg cagataaacc aggattagct ccaggtatga taggtgcata
     9061 tttagcaaaa gaagtaaatg caggttttat aggtggtatc atagcaggat ttatagcagg
     9121 atttgttgtt ttacaattaa agaaaattaa attagctcct acaatgagaa ctttaggttc
     9181 tatatttatt tatcctttag ttggtacatt gattacaggt ggtataattg tatttttaat
     9241 tggagaacct atagcttctt ttatgacatg gatgacaaac tggttaaatg gaatgtctgg
     9301 tgtttctaaa attcctttag gtggaatctt aggaggaatg atagcctcag atatgggtgg
     9361 ccctataaat aaggttgctg ctacttttgc ccaaactcaa gttgacacac ttccatattt
     9421 aatgggtgga gttggcgttg caatttgtat acctccaatt ggtttaggat tagctacatt
     9481 attgtttcca aaaaaattta gcaaggaaga aagagattca ggtaaagctt cactattaat
     9541 gggatgtgtt ggaattacag aaggagctat tccatttgca acatcagatc cagttagagt
     9601 aataccttgt attatggctg ggtcgattgt aggaaatatt atggcttttt tattaggatg
     9661 tttaaatcat gcaccctggg gaggtctgat agtgttacct gtagttgaca ataggttggg
     9721 gtatattgca tcagtattaa ctggagcagt agttgttgcg gtattaatga aattagtaaa
     9781 aaaagacgtg aaagaagatg aagaaattga agaagattta gatgaatcaa ttgaattaat
     9841 atttgaagaa ttataaatat aaatggaagg aatttgtata aaaaagtaaa tataaaacta
     9901 taaatttaaa ggagataaga atatgaaaat tgtagcagta acatcttgtc ctagtggagt
     9961 agcacatact tacatgtcgg ctgaatcact tgaattatca gccaaaaaat ttggtataga
    10021 aataaaggtt gagactcaag gaagctctgg aatagataat gaattgtcac ttaaagatat
    10081 tgatgaagca acttgtgtaa tattgactaa tgatgttgag attagaaata tggaacgttt
    10141 taaaggaaag aaggtagtaa gaatgagtgt atctgagata attaagaaat ctgatgcatt
    10201 aattaagaaa ataaaagata cttttcaata aaaattgatt tattttacat ataactcaaa
    10261 gatgttttta aagaaatttt tattaaaagc atctttgaaa atatcttaaa aatagaataa
    10321 aaggagatat agtcatgaaa aagttaccaa taaagactgt cgtgcaaaga ttactaaact
    10381 tacaagaaga ggggaaaagt gcaactcttt taggaattgg tcctatgtca ccaaacttgt
    10441 tacaagcaag ttttgaacta gcaaaagatg atgattttcc tttaatgttt atcgcaagta
    10501 gaaatcaagt agatgcagat gaactaggtg gcggttatgt aaatggttgg aatcaggaaa
    10561 cttttacaaa agatattaag aaggttgccg acaaagttgg atttgatggt ttatattatt
    10621 tatgtagaga ccatggtgga ccatggcaaa gagataaaga gagaaatgac catttgcctg
    10681 ttgatgaagc aatggagtta ggaaaaaaat cttatttagc ggacattgaa gcaggttttg
    10741 acctattgat gattgaccct acaaaagacc catttgaaat tggaaaagta attccactag
    10801 atgtagtact agaaagaact gtagaattaa ttgagtattg tgaaaacgag agaaaaagac
    10861 ttaatctacc agacataggt tatgaggttg gtacagagga aaccaatgga ggtctgactt
    10921 caactgagac gtatgaaaca tttataacta gactaaaggt tgaactcgat aatagaggtt
    10981 tgccaatgcc aacatttata gttggtcaaa ctggtacttt aactagaaag acagaacaag
    11041 taggaacttt taattttaga aatgcttatg atttagctca aatggctaag aaatatggag
    11101 ttggtttaaa agagcataat ggcgattact tagatgatgt aactttatta gaacatatac
    11161 catctcaaat tattgctaca aatgtagctc ctcaatatgg tactgaagaa actagggcat
    11221 atttaaaatt agcagaagtt gagtgtaaat tagaaaaaga gggcttagtt gagaaaactt
    11281 ctaatatcag acatgttctt cttgttaatg caattgaatg tggacgttgg agaaaatggg
    11341 tagtaggaga acaaaaaaat ctaactactg aagagatatt taaggatgaa gttttatcta
    11401 atgaaatact agatattgca ggacattata catttaataa tgatgatgtt aaaaaagaaa
    11461 tagaagtgct ttatgataat ttaagtaaga ataatattga tggtcaaaga tttgttgtag
    11521 accatattaa acgtcctttg cgtgattatg cagaatgcta taacttaaaa ggtgttacta
    11581 cgaggatttt aaataaataa taggacttat agtggtgagt attgttatac taggtattta
    11641 tagttataat tatacttacc actattaaat ttaggaggac taatattttg attaatttaa
    11701 tttttgatgt agatgatact ttatataatc aattaacgcc tttttacact gcatataata
    11761 aagtgttttc ttcaataaag gatatttcta ttgaagattt atatatgagt agtagaaagt
    11821 atagtgatga ggtatttcat atgactgaaa atggagaaat gccaatcaaa gaaatgcata
    11881 tttataggat aatgaaagct tttgaggagt tgggaaattc tattacagaa aaagatgcac
    11941 aaagttttca agatgagtat atatatcaac aatcacaaat tacactaata ccagaagttg
    12001 agaggatttt aaatttttct aaagaaagaa atattaatct aggaattata actaatggcc
    12061 catcaaacca tcaaagaatg aagttaaagc agttaaatat tgaaaactgg gttgataaaa
    12121 gtaatatttt tatttctagt gaggttggat tttctaagcc agatactaat atttttagag
    12181 tagctgagaa tgttatgaat ttagatagag aaaatacata ttatgtgggt gattcttata
    12241 gaaatgatgt attaggtgct aaaaaagctg gttggaagag tatatggttg aaccatagag
    12301 gacatgaggt agaagagttg ttttacaaac ctgattttgt aattttggaa cataaggatt
    12361 tgatatcttt atttataaaa atatgtagta aaaaatgtta ataatagaat tgtataaaac
    12421 aattaaaata tgtgtaaaat cataaattta agtta
//
LOCUS       DACXMZ010000106        12384 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.107, whole genome shotgun sequence.
ACCESSION   DACXMZ010000106 DACXMZ010000000
VERSION     DACXMZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.107"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            168..266
                     /locus_tag="JD016_11405"
                     /pseudo
     CDS             168..266
                     /locus_tag="JD016_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            268..1386
                     /locus_tag="JD016_11410"
     CDS             268..1386
                     /locus_tag="JD016_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAU5000240.1"
                     /translation="MVVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLDNAYKKFFKKKAGFPKF
                     KSKKINRFSYKTNFTNGNIVYCGQHIKLPKLGMVKIRDKQVPQGRILNATISKEPSGR
                     YYVSLCCTDVDIEVFENTNNQIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            1534..2598
                     /locus_tag="JD016_11415"
     CDS             1534..2598
                     /locus_tag="JD016_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000241.1"
                     /translation="MFLKLIFKYNTLDFSRAYFDLTDWADNPKDISYYLSNGTFGDLM
                     DSIDENASKMSLSDIAKLEKKIAKKHINLWNEGKIDFSTTRFSSIVPMLQNAGVNFHF
                     IYPDIDLLKNTFNNLIKDIHLHRMKGNQPSVIYITVKDTNYDDSTLDQVYKILNSIKK
                     DKLTDFIIEKNSNSFKIFTKCETIKSLTKNFTACYIKESIENSTNIKVCVGYGIGNDI
                     KQAMSSAISANKESKINHLNYSFLVNENYETIGPLLCDKLLTVSNKVTPHINTIAKSS
                     KLSTLTIQKIISVTEILQRDEVTPKELSSCLNVTVRTANRFLSSLLKSGNATILYEKQ
                     NSTKGRPERVYKLFISENKY"
     gene            complement(2726..3916)
                     /locus_tag="JD016_11420"
     CDS             complement(2726..3916)
                     /locus_tag="JD016_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAU5000242.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNIPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(4022..5401)
                     /locus_tag="JD016_11425"
     CDS             complement(4022..5401)
                     /locus_tag="JD016_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAU5000243.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(5432..6640)
                     /locus_tag="JD016_11430"
     CDS             complement(5432..6640)
                     /locus_tag="JD016_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000244.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            complement(7665..9119)
                     /locus_tag="JD016_11435"
     CDS             complement(7665..9119)
                     /locus_tag="JD016_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAU5000245.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            complement(9139..11010)
                     /locus_tag="JD016_11440"
     CDS             complement(9139..11010)
                     /locus_tag="JD016_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit EIIBCA"
                     /protein_id="HAU5000246.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            complement(11000..11860)
                     /locus_tag="JD016_11445"
     CDS             complement(11000..11860)
                     /locus_tag="JD016_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU5000247.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
BASE COUNT         4284 a         2120 c         1456 g         4524 t
ORIGIN      
        1 ggttcttata tatgttattt aaaattaaat ataaaaaacc ttcaaaatac ttgttttaag
       61 taattgcata ttttaatatt ataataggtg atgaaaaatg gaagttactc atggtagagg
      121 atatgcatat agtatgtcaa atatagacat ccagcattat ggggagacat ctttggaatc
      181 ctagttattt cgttgctact gtatcagaaa atacagagga gcagataaga aaatatatta
      241 ataatcagaa aaaacaagta aagtgaggtg gtagtagtgg aaaaagctta taagtttaga
      301 atgtatccaa ataaaaagca acaagagtta attaacaaaa cttttggatg ttgtagattt
      361 gtatataata agtatcttgc taaaagaata gatgtttata aaaacaataa agaaactttt
      421 acttataaac agtgtagttc tgatttgacc aatttaaaaa aagaattaaa ttggcttaaa
      481 gaacctgaca aattttcact acaaaacgcc ttaaaagatt tagataatgc atataaaaaa
      541 ttctttaaaa agaaggcagg atttcctaaa tttaaatcaa agaaaattaa tagattttca
      601 tataaaacta actttacaaa tggaaatatc gtgtattgtg gtcaacatat aaaactacct
      661 aaacttggta tggttaagat aagggataaa caagtacctc aagggagaat acttaatgct
      721 actatatcta aagagccaag tggtagatat tatgtatcat tatgctgtac tgatgtagat
      781 attgaagtat ttgaaaatac taataatcag ataggtttag atttaggaat taaagaattt
      841 tgtatctcaa gttgtggaga ctttatagaa aatcctaagt atcttaagaa gtcattaaac
      901 aaacttgcta aattacaaag agaattatcg agaaagacaa tcggtagttt aaatagaaat
      961 aaagcaagat taaaagttgc aagacttcaa gaacatatag ctaatcaaag aaatgatttt
     1021 ctacaaaaac tatctactaa attaataaaa gagaatgata ttatttgcat agaagattta
     1081 caagtaaaga atatgattag aaatcgtaaa ctatctcgct taatttctga tgtatcatgg
     1141 tcagagttta ttcgtcaatt agaatacaag gctaattggc atggtagaca aattgtaaag
     1201 gtaggtaaat tctttgcaag ttcacaaata tgcaataaat gtgggtataa aaacgaggaa
     1261 gtgaaggact taaatataag agaatggatt tgtcctagtt gtaatgaaac tcacgataga
     1321 gatataaatg caagtataaa tatattaaaa gaaggattaa gactaataac aattcaaaat
     1381 aaataactta tgtaagaacc gtaggaacta cggggatggc ctgtggagaa ttagtaagac
     1441 atattttagt atgcaaaatt ctatgaagca ggaaccctgt gactttagtc atgggaggtt
     1501 cagtttggta ctgacttaca aagttactat gatatgtttc taaagcttat ctttaaatat
     1561 aatacattag atttttccag agcatacttt gacttaacag actgggcaga taatccaaaa
     1621 gatatatcat attatttatc aaatggcaca tttggagact taatggatag tattgatgaa
     1681 aatgcatcta aaatgagttt atctgacata gcaaaacttg aaaaaaagat tgcaaaaaaa
     1741 catattaatc tatggaatga agggaaaatc gatttttcta ctactcgttt tagtagtatt
     1801 gttcctatgt tacaaaatgc aggagtaaac tttcatttca tataccctga tatagattta
     1861 ttaaaaaata cttttaataa cttaataaaa gatattcatt tacatagaat gaagggaaat
     1921 caaccaagtg taatatatat aacagttaaa gatacaaatt atgatgactc tactctagac
     1981 caagtttata aaatattaaa tagtattaaa aaagataaat taaccgattt tataattgaa
     2041 aaaaatagta attcttttaa aattttcact aaatgtgaaa cgattaaatc attaactaaa
     2101 aattttactg catgttatat aaaagaatct attgaaaata gtacaaatat taaagtatgt
     2161 gttggatatg gaattggtaa tgatataaag caagctatgt ctagtgccat aagtgctaat
     2221 aaggaatcta aaataaatca tttaaattac agtttcttag ttaacgaaaa ttatgagaca
     2281 ataggacctt tactgtgtga taagttactt actgtttcaa ataaagtaac tccacatata
     2341 aatacaattg ccaaaagttc taaattgtct actcttacta ttcaaaagat tatatctgtt
     2401 actgaaattc tacaaagaga tgaagttact cctaaggaat tatcttcatg tttaaatgta
     2461 acagttagaa ctgctaatag atttctaagt agtctcttaa agtctggaaa cgctactata
     2521 ttatatgaaa aacaaaactc aacaaaaggt agacctgaaa gggtatacaa attatttata
     2581 tcagaaaata aatattaaaa tccaaaatgc ataaaaatat tttatcatct aatattaagt
     2641 aaaattatat atgactttct aaaaggtata tctttgcatc tttttaagat atacctttta
     2701 tgatatttaa taatatttta tgttattatc tactaaaatt attttctaac cattttactg
     2761 cacaatgagc aaataaagct gaaccataaa ctaatccctc ttcattaaaa attgctttag
     2821 gattatgcat accaaaatta tatccctgac taatacttcc cattccaatc cataaaaata
     2881 ctcctggaac ccttagtaaa atctctgaaa aatcttctga acccattgca gatggaaagt
     2941 ctattacatt ttcatttcct ataacttctt taacataaga tacaatctca tctcctacat
     3001 ccaaatcatt tataactgca ggagtcccca ttttaaattc cactttagca tttcctccaa
     3061 atgatttagc tattcctgta gatatttcaa taattctatt ttttaatttt tctctaacaa
     3121 attggtcaaa tgttcttaaa gttcctgaca tagatgcttt atttggtata gcatttggta
     3181 cgtttcctgc attaaacata ccaatagtta aaactacagg ttctttctga ggtttttctc
     3241 ttgaaagtat agtgtttaat gctatttgta tatgcgacat tatgtttatt gggtccactc
     3301 caaattcagg gcttgctcca tgagttgctt ttccttctac tgttatttca aatatatccg
     3361 cagatgccat agctggacca cgtgaataag ctacttgacc tgtgttaaga aatttaccca
     3421 ccatactgtg aaatgccata gctgcatcga cctgaggatt ttctaatata ccatcatcaa
     3481 tcattgattt tgcacctctg gtagtctctt catctggttc aaatacaaac tttactctac
     3541 ctttaagtaa gtgtttatta tccataagta ttttagctgc tcctaagagc attgctgtgt
     3601 gattatcgtg tccacaacta tgagatgcat ctttattctt acttttaaaa ggtaaatccg
     3661 tctcctccat aataggaagt gcatccatat ctgcccttaa taataaggtc tttccaccat
     3721 tttcactgtc taaaacagct actattccat tattagacgt caatttcgga gaatatccca
     3781 ttttttctaa agtactggta acaaaggtta ctgttttcgg tatattgcaa ccaatttcgg
     3841 gattttcatg taaatatctt ctccatctaa taatttcccc ttctattgac tgcgcttctt
     3901 taaaaaaatt tttcatattc cttcctccta atcagatagt cagcaaaatc tcactttctt
     3961 ttagtaatga aatgatgctg atataaaaat tataatttta tatattaaat ttcatgtctt
     4021 tttaaggagt tggaatatca tctggaatag gacatttata tgttttacca ttcatagatt
     4081 tatcaaattc ttcttttgcc tttttagcta tctctggatt atttaatatt ttagctccaa
     4141 acatagccat aatctttgat gcatatatca tacctttttc tcctatagaa cttcctgcac
     4201 aagatgtaaa ctgccaacta tgacctggtg ctcctagatt cgtacaccct gtaaaaaagt
     4261 atgctgtcgg aactatatgc attacatctc caatatcagt agaaccataa ctattaaagt
     4321 ttgtaacatt tcctccacca taatatagat gagttccttc tggcaaatta tatttactta
     4381 tcatagcctt atattgttgt gggctttgtc tatctaattc agctgcaaaa tccaattcct
     4441 ctcgagtcca aggctcttgt ttaatctcat tcatacattc tgatacaaga tttgctaaaa
     4501 catgattgtt taaagtatta taacaacctc ctaaaaactc tacttcaact tcagtttcag
     4561 tcatcattgc agctcctttt gctaccttta caagtctttc atatgtactt tctacagctt
     4621 ctctacttaa agctcttgta taataccata ctgatgcttt atcaggaact atgttgggtg
     4681 ctacgcctcc atctgtaatt gtataatgaa tccttacatc agaagtaaca tgttctctca
     4741 aataattagc tcccacatta gtaagttcca cagcatctag tgcacttctt ccattttgag
     4801 ggtctgagcc tgcatgcgca gtctttccct taaagtgaaa ctttacagag ttaacagctg
     4861 ttgaagtacc aattgtagct tcactaattt tatttggatg aaaatttata gctaagtcta
     4921 acccttcaaa cgctccacct ctagccataa atcctttacc tgttaattgt tcttctcctg
     4981 gacatgcata aaatactata gtcccactta agttattttc tatcatctct cttttaagcc
     5041 ctactactgc ccctacatgt gctgtacata ataaattatg cccacatcca tgaccatatg
     5101 cacccaattc aaatgcttct tgctttgttg aaactttttg atttaaacca ggtaatgcat
     5161 caaattctcc cataaaacct ataactggtt tacctgaacc aaaactagct ctaatagccg
     5221 tggctaaacc tcctacacct acttccacat caaatccttc acttcttaga aagtttgctg
     5281 tattttcaca tgctttaaat tctttaaatg ctttttctgg attttcccat attttcttac
     5341 ttaaatcatc aagtttgaat ctttttttat ctatctcatt tatagatata cttcctatca
     5401 ttttctattc ctcctataaa tctatttact atcaaccaaa tataattgga cctattatac
     5461 tagcaaatgc cactgatgca atggttacag taacaaatcc tcctataatc atctttggta
     5521 ataaataatc tgttgccctt tgtttttctt cttcagttcc ttccatagca tcaatacctt
     5581 cattggaaat tatctgtgtt gcaggataag ctagcataca agttacacca attgcagctg
     5641 acattcttgg agaatatccc aatagttttc ctacaattgc agtaaataat attataaata
     5701 cagaacctac aacaagtatt cctatagttg gaacaatcat cccaaacaat gctgttggag
     5761 ttaccttagc aagtcctcca ggtaacatca acatacaacc aaacattata attccattag
     5821 attgagattt atctaaaatt cctttttcta aaaaacctat tcgtgtaaaa atcaacccta
     5881 ctaacaaaca tgctatgttg ggatttaaat aatagtttgc aggggtaaat cctggtatta
     5941 aagtagccaa tcctacaaaa ttaccaatca tagcaacaaa tgaaagcttt gcaagataca
     6001 ctaaagtaga tgatttttta cctaaatctt ctttaaaaat tctcatactt ggaagtttaa
     6061 aattactttt tgactcatct ttcaaaaaat ccccattatc tcttttaaat tttaattctt
     6121 tatttataca atatgtagtt atcggaatac caaagaactt ttgaaatgat aatactagaa
     6181 ttacaaatgc agcaagttca ggtctatttg cagcttctgc aacagttgtt acgataatcc
     6241 cagcaacagt agaacctgct attggaggtg ctgctattaa agcatactct ctgccaaata
     6301 acatactacc cactgttaaa cagccaacac ctattcctac aatacctact aatgcaatta
     6361 caaccgtttt ccattcatga cataagtctt caagatttat aagtgttcct aagttagtta
     6421 ttaataaagc tataccaaag ttgctcattg cagctaatat ccctgatgct cctgtaatgt
     6481 cttttggtaa aatatttgtc caaaatccta gtaaaaatat aatacataca aataacaatg
     6541 ttgaaattgc accttttgtt ttcttagata aaaactcact aaaagcataa atcattacac
     6601 atacagtaaa gcaagtaagt ggattccaaa aattttccat tgttgtttcc tcctaaatat
     6661 aataattttt tatcaataaa acaataatta taaaatgtat attatgcagt tattcttaac
     6721 tacatatggt atagcttgat attttttgat gatattatat acgtcctaaa ttattcctaa
     6781 aatatctaaa taaatataac tagagttaac tctctaaata ttaactatag ttatcaactt
     6841 actagatttc tttttgattt ttactttcta cataatttta cgagttagaa ttgtacttca
     6901 actactctct atattagtat acatgttgga agacttatat ttatttaaat attaaatata
     6961 gaatacattt ttataaacgt cttaaattat tcttaaaata tctaaaatga aataataata
     7021 tacccattaa accaatatta tcagaatata cttcacttta agtcttcttt atatagaaat
     7081 tttatctatt agtgcatccc ctcctttgta aattagacta tttctaaacg aatttagata
     7141 tcttattcct aggaactatt actaaaaaca aaaattattt attatattaa atcaatgata
     7201 agccatactt taaaattctt tctatacaag tataatttat catataatga catctatacc
     7261 tttatcttta tagtagtcct aaattattct taaatttaca ataaaaataa tttcactatt
     7321 tataaaaata cttgttttta tattgtcaaa ctattcttaa tatagattat aattttaaat
     7381 cttttctttg tcaatatatt ctgacatttt tccagaaaat ttataaagtt atatagtgag
     7441 taaatcatac tatttttata taatgagtaa attatactat gaatatgtct aaatatttac
     7501 ttaagtataa acataaaaaa tgtgtattct cctttttata gtaattttag ttgccattac
     7561 ttttaataag aatacacact ttactttaaa gaccctatac tctattttaa atgctttact
     7621 agacactaat taacattagt tcctaacttc tatttatctt attcctaccc tactactata
     7681 tccgtagaaa aatctaaaac ttctccatta gattttatta cttctttgta ccaatcaaat
     7741 gattttttct tatatctctt aaaacttcct ttgccttcat catctaagtc aacatagata
     7801 aatccatatc tcttactcat ctgacctgta gttgcactga ctaaatcaat tggtccccac
     7861 atcaaatatc caaaacattc aacattatcc tcttcaattg cagacttcat ttgagataga
     7921 tgcttactta aatattcaat tctataatca tcatttatag ttccatcttc ttcgatttta
     7981 tcatatgccc ctaaaccatt ttctgtaatt aagataggta attgatactt tctgtaaata
     8041 tagttcaagg taaatcttaa acctataggg tctattgccc acccccactt actttgttcc
     8101 aaatatggat tttgaatccc accaaataaa gatttatcac ctgataaatt ttcttttgga
     8161 gtagatacac ttgaaaaata ataattaagt cctataaaat ctaacttacc ttctttaaat
     8221 gcttttttgt cttcatcagt cacttcaata gttatattat ttttttcata ttcttttaat
     8281 ttatattgtg gaaacttccc attgcacatt gcatcaattt gataataatc acggtcatta
     8341 tctaaaaatg catttaacac attactagga ttacaattgt atggataaat tggatttagt
     8401 ccaaatacac atccaacttt attgttggga ttgatttctc tagccaactt tgcaaccttt
     8461 acacccgcca aagtcatatt ataccctaca tttgccaatg tttgaacctt attttctatt
     8521 tcactatact taatacctgc tatcatataa gtaaaaatat ccgagtgctc tgtttgaggg
     8581 tcaatatggt tcatctcatt aaatgtaacc cagtacttta ccttatcatc aaatcttctt
     8641 attacagtct cactgtactt taaatacaaa tctataagtt ttctgttatt ccatgaacca
     8701 tatttatgta ctaaattcat aggtaattca aaatgatata gtgttacaat aggttctata
     8761 ttattttcta ttaatgtatc tatcagacct tcatagtatt taatccctaa ttcattagga
     8821 ttttcatcat ctccatttgg aaaaattctt gaccaatcta tagatattct taatgcacta
     8881 aatcccattt ctttaaataa agctatatct tctttatatc tattataaaa atctattcca
     8941 ttatgagatg gataatccaa tttttcttct attttatcag taattactct tggcgtttca
     9001 taagagccct tagttacaaa atccataatt gcaggtcctt tgttatcact atcccatgaa
     9061 ccttcacatt gatgggctgc aatacttcct ccccagaaaa atgtatcttt aatattcatg
     9121 aattttccct cctaaaattt atactattac agttaataat tcttctgatt catcatttga
     9181 tgagattaca tctaaataat cgctagtatt tgtaactaca gttattatag tagaatcaaa
     9241 gccttcctct ataattttat cagattcaaa tactgctaaa gcatctcctt ttttaaccct
     9301 ttgattttgt gttacaagac tttcaaagaa ttttccatct aagtttacag tatctatccc
     9361 aatatgaatt aatatttcta atccttcatc agtcactata ccatatgcat gtttagttgg
     9421 aaatactata gttatagttc catctacagg agatataaca gttgattttg attcactagg
     9481 tataactcca acccctttac ctaatgctcc agatgaaaat gctttgtcat taacttcccc
     9541 aagtgcaatt acctgacctt ttgcaacatt tccaatacta atagttcttg aattgttctc
     9601 gttgattatt agttctaatt ctttttcatt atcaccctta acttcttctt tttcatctga
     9661 aggtattcct agtaaatagg aagctgctgt tgctgctaca aatgatacta ctacacttat
     9721 tatcataaat gtaaagttag atacttctcc tccaacaaga tatgttggta atgcagctaa
     9781 tccccaaacc attccatatg ttttaacttg agctaaacct gctattaaac cacctatacc
     9841 tccacctatc attactgcta caaatggtct tctatatttc ataaacacac caaacactga
     9901 aggttctgta actcccataa gtgcactaaa acttactgta ccaaatagtt gtttttgttc
     9961 tttattccta gtttttaaga aatatccaaa cattgcacca caaactgcaa tatcagatat
    10021 tgttgcagcg cccataaaaa ttgggtcata ccccaatgtt gtcacagaat ttaaagatat
    10081 aggcatagta aagtttgctg caccaaaaac tattaagagt ggttgtaaag ctgcatacag
    10141 tgcaacaaca ctccaattac ccaatgtatc aattaaaaac atacatgcat agtttaatcc
    10201 atcaccaatc cacataccaa taggtccaaa tacagttaat gtaactgata gagttattat
    10261 taaagataat actggaacta aaaaatagtg tagtgcttta ggtaatattt ttttcaaacc
    10321 ttcaattata attcccattg cccatactcc taaaagtatt ggaataaaac tgtttgcata
    10381 agttgtagcc tgtaaattta taccaaataa acttaatcct tctacaccat ctattgatga
    10441 agataataac gtaactgcta acactactgc tatataggga tttacattaa gtctttttgc
    10501 tgatgctatt gcaattaata ctggtaagaa gtgaaatgta gcacttctta atgtatctaa
    10561 tatttgatat gtaggactgt cactctgaag tatgccagac atatttaaaa gtgctaatat
    10621 acttgcaagt ataccagctg caagtagtac ttcaattact gaagtcatag tctctgagac
    10681 taccacaaag atggaattaa atataccttt gatacctcta attccctttt ttctttcact
    10741 ttttatactt gattcacttg aacttaaacc tatcatagac ataaactcgt tatatacatc
    10801 tacaacatga gtacctatta taatttgata agcaacatca ttagaaacta catcaattac
    10861 tccttttaat tttttaattt cttctgtttg agcgatactc ctatcattta aagtaaatct
    10921 taaacgagta acacaatgcg ctaccgactt tatattttct tttcccccta taagttttat
    10981 tatattttca tttaaatctt tatatttcat ctgattttgc ctccagtctt tgagtcaccc
    11041 tatttatatg caacattaaa taagtttctt cacttataga tatatcgaca ttaaattgtg
    11101 attttatata ttttcttatc ttttgaacac ttccaaatga ctttggataa agttgactta
    11161 cttgtttata taaatctata gaattatctt catctattga agcttcatct gacataattc
    11221 tttggataaa gtactgtaga tgagtagtaa atctcatata atttgttgaa gtttcatcta
    11281 gctctacatt aaaatgatat tttattatag aaactatatc tgctaaagtt ctcaactcta
    11341 caattgtact atcatgagac tctttattta cctccatatt tacaaaatga agtgctattg
    11401 atactgcctc atcatcagga agattaattc ccttactatc accaattaat tttagtgcat
    11461 gcattccaat ttcaaaatgc tttggataaa atcttttaac attccatacc aaaggacttt
    11521 ttataaattg attcttttca catcttttaa gtaaaaactc taaatggtct agtaaggtta
    11581 agcttagata atcacttgac tttatattta aagctttctc accatattca attatttcat
    11641 taataagaac tatattattg gagtctgagt tttgtagtag ataactaaaa tgttctagca
    11701 tatcatatga atctagtata tatgttctct ctatttcatt ttcatttatt acttgtccca
    11761 ctcttttttt aaagccaatt cctttaacaa atacaataat ttcattttta cctcttttta
    11821 ctaaggctac attattattt aaaatctgaa ttattttcat atattcctcc tttcaataaa
    11881 aagaactcaa gcatatatgt atagagtatc cctctatttt tcacatatat acttgagttc
    11941 tgcctgctaa tcagtaacat actcaaaatt atgactacgt cgccataatt aaaattcttt
    12001 tttgtttaat ttcatatttt atatgttata ctatccatat aaatctgtca acggtttcct
    12061 agatattttt ttattctata actacaagct ctgttatacg attatctgac aaggtttata
    12121 tactaaattg atactatttt accgtcacta atatttataa tattgtctga accattatct
    12181 attttactat tatgaattat catagtaatg cttttaaata taatgaactg tatgttaaca
    12241 aataaattct taagatatat taacatataa agtcttaagt ttagttttat ttaatcacta
    12301 ttaagatata ttaataggta aagcttcaaa tttagtttca tttaaatgct agtaagatat
    12361 actaatatat aaatacttaa attt
//
LOCUS       DACXMZ010000107        12327 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.108, whole genome shotgun sequence.
ACCESSION   DACXMZ010000107 DACXMZ010000000
VERSION     DACXMZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.108"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            382..744
                     /locus_tag="JD016_11450"
     CDS             382..744
                     /locus_tag="JD016_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAU5000248.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLDVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            809..1426
                     /locus_tag="JD016_11455"
     CDS             809..1426
                     /locus_tag="JD016_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HAU5000249.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            1423..1908
                     /locus_tag="JD016_11460"
     CDS             1423..1908
                     /locus_tag="JD016_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HAU5000250.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTDTGELLVDSINKGKKK
                     L"
     gene            1951..2469
                     /locus_tag="JD016_11465"
     CDS             1951..2469
                     /locus_tag="JD016_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HAU5000251.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFRVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            2605..3564
                     /locus_tag="JD016_11470"
     CDS             2605..3564
                     /locus_tag="JD016_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAU5000252.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            3590..5293
                     /locus_tag="JD016_11475"
     CDS             3590..5293
                     /locus_tag="JD016_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HAU5000253.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            5506..7608
                     /locus_tag="JD016_11480"
     CDS             5506..7608
                     /locus_tag="JD016_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HAU5000254.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            7621..8061
                     /locus_tag="JD016_11485"
     CDS             7621..8061
                     /locus_tag="JD016_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HAU5000255.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSM"
     gene            8097..8900
                     /locus_tag="JD016_11490"
     CDS             8097..8900
                     /locus_tag="JD016_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HAU5000256.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            8940..9536
                     /locus_tag="JD016_11495"
     CDS             8940..9536
                     /locus_tag="JD016_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HAU5000257.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            9642..10505
                     /locus_tag="JD016_11500"
     CDS             9642..10505
                     /locus_tag="JD016_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAU5000258.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKYNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            10572..11561
                     /locus_tag="JD016_11505"
     CDS             10572..11561
                     /locus_tag="JD016_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAU5000259.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            11653..12264
                     /locus_tag="JD016_11510"
     CDS             11653..12264
                     /locus_tag="JD016_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAU5000260.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         4979 a         1218 c         2317 g         3813 t
ORIGIN      
        1 aactatctat tagatattga gaaagtcaaa tcctatatga atctacagaa atgattttaa
       61 tgaatctgaa aatttttttt atacaaacat aaaaaaggta taaagttgtt ttattataat
      121 ttaattactt ttgataactt tttatgtttt atacaattat atttttaata actcgtcaaa
      181 acgccaaaca tatattacaa tagatatata agtttttttg ttgacaaatt atgttaagag
      241 aattaagtat aatatggcat aaataattac ttttatttat caatgaaatg tatttattgt
      301 gtagcgaaaa atagtcagat agtatagaca ataactgaag ataaaagcgt ttattgttgt
      361 acatatggag gagttagttt tatggaaaat aaaattttga tagtagatga tgctattttt
      421 atgagaatga taattaaaga agctctgagt aaaagtggct ataataattt aatagaagca
      481 aatgatggag aagaagcatg taatatattt aaatatgaaa agcctgattt gatgttgctt
      541 gatattacaa tgccaaaaaa agatggttta gatgtgctaa gagaaataaa aaagttggat
      601 tcaagtgcta aggttgtaat gtgctctgca ataggacaag agaatatgat tgtagaagcc
      661 ataaaacttg gagcgttaga ttttatagtg aagcccttca aaacagaaac attagttaaa
      721 gttgtagata atgctttaaa ataaaagtgt aaatatacac taatacaatg aaaagcaaat
      781 aaatgcaata ataaagggag gggtttatat tataaattat ttagaacttg atgatgttca
      841 tatagatgct cttagagaaa ttggtaatat aggctctggg aatgcaatta ctgcacttgc
      901 atccatgata aatagcaatg tagaagtatt aatacctatg gtcaagatac ttgaatataa
      961 tgaagcgaca aatttgcttg gtggtccaga aaacaaagta gtatgtattt tactcgatat
     1021 gaaaggtgat atcaatggaa tgtttatgtt tttgttggat gaatcaataa ctcaacttat
     1081 gctaagttct ctttttaata aagaagaagc ttttttagat gagattgagg caattgaaat
     1141 ttcagcaatt aaggaaatag gaaatattat ggcaagttca tatgtaaatg ctattgcatc
     1201 tatgttaaac atgactataa gtgtttcaat tccagatata tgtattgata tggttggagc
     1261 tgttctaaat gtacctatga taagattttc agatgttggt gacaaagtct tatttataga
     1321 aaataaattt aaaatgagtg ataattattt tactagtcac attttgatga ttccagaaat
     1381 gagctcactt agagaaatac tcgtgaggtt gggattagaa gtatgaatag agagatagta
     1441 gtgaatattg cagatatgaa gatagcctat agaccaaatg ttttagttac ttatgcactt
     1501 ggttcttgtg taggagtatg tttgatagat aaagtagctg gaattggtgg aatgttacac
     1561 gttatgttgc catatagtaa agatgctatt aacattgaaa ataagtgtaa atttgctgac
     1621 acaggaataa atgagctcat taaatctatg gagaatatag gtgcaaatag aatatatata
     1681 aaagcaaaaa ttgctggagg tgctcagatg tttttaggaa gtagcaattc agtaattgca
     1741 agtataggac ataggaatgt tgaggcagta aaaaaaactc ttttaggatt aaatattcca
     1801 atattagctg aggacacagg tatgaattat ggtagaacaa tacgttttta cactgatacg
     1861 ggtgaattac ttgtagattc aataaataaa ggtaagaaga aattataata tatatttcat
     1921 agaaagtgta ttttaggagg gaggtattaa atgagatttc cagatgaccc atatttagat
     1981 tatggactaa ttgatgaagg aattaagtat ctaaagttta gagtagatga gcaagacttt
     2041 ggggttgaac ttgaaaaggt aatagaaatt actggaaatc aagaaattat atttattcca
     2101 gaactaccta catattctaa aggcataata aatctaagag gaaaaatcat tccgataata
     2161 gatatgcgtt tgagacttaa aaaagatgaa ttaatgacta caaattgcat gtatatagta
     2221 gtaactgaaa taaaggactt ggtagttgga tttgtggttg atagagttga tgatatagta
     2281 agcttagaaa aaagtaaaat atcacctcca ccaagagtta ctatagaata ttcggattat
     2341 tttgtaagtg gagttggaga attagaaggt aaaataatta ctttactaga tttagagaaa
     2401 cttttgacag ataaagatga agttttaata gatgaattaa tcaatgggtt tgattgcact
     2461 atcatgtaac atagggatgt agttagagtg ttattataaa aagattgtga tgagaacatt
     2521 taagaatata gtggtataat tattaagtat aaaaatttta aatcattaaa atttgaatat
     2581 attatgttta agagaggttt gtatatgaaa aataagcaac agggaacaat tttagtcgtt
     2641 gatgatagtg ttctgatgtg tgacttaata gataaaacac ttacaaaaga taattttaag
     2701 gttaaaaaag catttaatgc aatagaggca aaagaattta ttgaagaatt tataccagac
     2761 attattttgc ttgatataat tttaccagat aactctggtt ttgagttttg taaggaagta
     2821 aaatctaatg ctcgtacagc agatatacct ataattttta taacttcaaa aaatacagat
     2881 acagacatag taaaaggatt tggtgtagga gcaattgact atatggtgaa accttttagt
     2941 atgacagaat taaaagctcg tgttatagct catcttgaag taaagaaatc tcaagataaa
     3001 ttaaaaaaga taaataatga actggaatct tctcttgaaa aattgaatag actcgttgta
     3061 agagattatc ttacaggtct ttacaatagg cgacatataa ttaatcagct tatggagcaa
     3121 agaagaatta ataaatatag tgaaacatca atatccctta tccttggtga tattgatgac
     3181 tttaaggtta tcaatgatac ttatgggcat gaagcagggg atttcgtgtt aacagtaata
     3241 tcaagtataa taaaaaagaa ttgtagacaa attgatactg tatcaagatg gggtggagaa
     3301 gagtttttga taatgctcac aaatactcca atagaaggtg ctaagatact ttcagaaaga
     3361 atccgtaaag agataaagga gtttgatttc aactataaag aacataaaat aaagtgcact
     3421 attacattag gagtagtaga agtaaactca aatatatcat tagaagaaaa catatcctta
     3481 gcagataaag caatgtatga gggtaaaaac ttaggaaaag attgtagcgt agtgagtaca
     3541 atgaaaggat taaaaaagat ataaacttac tttatgaagg agtacagata tgaaaagaga
     3601 agtaaagctt tctaaattag caattgtatt gttgattatt atagtattgg ctgttctagc
     3661 agggattttt tcttctattt catcttataa agcatttgaa aaagtagaaa aaagtattaa
     3721 tgatatgaat aatttgagga ctcaaagtca gtatataaaa gatacaacta gagagataag
     3781 tgatgtatta agaagatatg tatttacagg agaaaaaaag aataaagata tttatgaaaa
     3841 agaattatac ggaaaaacaa gagaatcagc tttaaacaaa ttagaaaaaa ttggtcttac
     3901 aaagagcgag tcaaataaaa tcttagaagc aaaagaaata tcagattctt tagtaccaac
     3961 agaacaaagt gcaataaagg cagtcgaaag taatgataag agtaaagctc aaagtataat
     4021 ttttggtagt gactatataa actcccaaga gaaaatggat tctttaataa atgaatttca
     4081 agaggatata gttacccgtt caaattccaa aattgtagag gtaaaaaaag aattacaaag
     4141 tctaatatta agaacaatat ttatactttc ccttttaata atattgacaa taatagaata
     4201 tacagtaata aaattaaaaa tagtgattcc tattaataaa gtagaaaaac acttttcaag
     4261 aattgcatct ggggacttaa gaacaagtat agacgttgaa gaaagtaaat ctgaaatagg
     4321 actattagtg agttctgtca aaaagatgca aagtatgctt ttaaattaca tagaccttat
     4381 agacagtaca ttaactagta ttgcaaaagg agatttaaga gtagagatag accaagaatt
     4441 tataggtgat tttaaatcaa ttaaaatatc tttagaaagt ataataaact cttttaatgg
     4501 tgatttttct gaaataaatt tatcagcaga acaagttgca agtgcttcag aacaagttgc
     4561 agcaggtgca caagctcttt cacagggtgc tactgaacaa gctagttcag tagaagaatt
     4621 agcttccacg attaatgaga tatcagatgg aattaagaag tgtgcagata gtgctagtct
     4681 tgcatgtgag gtatctgacc aatctgcatc acaagttggt ttcggaagta gatgtatgga
     4741 tgatatgatg acatcaatgg gtgaaataag tgaaaaatcg agtgaaatat caaaaataat
     4801 caaaacaata gatgagatag cattccaaac taatattctt gcattaaatg cagctgttga
     4861 agcagcaaga gcaggtcaat atggtagagg atttgcagtt gtagcagatg aagttaggtc
     4921 tttggctggt aaaagtgcaa aagcagctca aaatacagct attctcatag aagaagctat
     4981 taaaacagta gaaaatggtg tcagaattgc taaagagact gcaaatgcat tggacttggt
     5041 agtaaatggt gtagaaaaaa ctactaatta tattaatggt atttcttcgg ctgtacatag
     5101 tcaagaagta gcaattacac agattttatc tggaatagag caaatttcca tagtagtcca
     5161 aatgaactca gcaacagcag aggaaagtgc agcagcctca gaagagatgt ctgctcaagc
     5221 tcaaatttta aaagaattag tagaaaaatt tcatctaaag gaaagtgaag taagagaatc
     5281 atcatgggtt taaattatga gatgaatatt gaagtaagag aattatcatg tatttaaatt
     5341 atgagatgta tatatattga agtaagagaa tgattgtgta tttaaattat gaggtacata
     5401 tagtgagata agagaatcat caaggattta aattatgaga tgcatatata atagaaaatt
     5461 atttgtaggt cgtaggtgat aaatgtgaat tatattgaac cgagtatgag acaaatgtta
     5521 gatatgttta tatttgagac aagtttatta ctcgaacaat tagatgaaat tatgattcaa
     5581 atagaaaaag agaaagtatt tactcataac aacattaatg aaacttttag aataatgcat
     5641 acgataaaag gttcagcatc tatgatgggg ttagacaata tatctgcttt agcacatagt
     5701 gtagaagact tgttttatgc tatacgtgaa ggtaaaatag aagttttaga caatgaccta
     5761 ttatttgatt tagtctttca atcattagat tttataagaa gagatataga tgagttacaa
     5821 tcaagaggtt gtatatcttc tgattactct aattttattg aaggaattga agagtatgtg
     5881 agtactttgg aattatcaag ttctatgaaa tcaaaggata atgcaaattt agaaagtaca
     5941 gacgaaaaag aagtaataga ttcaaatttt gaatacaaag aagactctga tgtagtaaaa
     6001 ataatctttg aacaaggttg catgatggaa aatgtgagag ctttcatgct tgtatttgaa
     6061 cttagagatt actgtgaatt tatagatttt tatccaaaag atgtagaaac aaatgcagag
     6121 tctacagagt atattaaaag ttatgggttt tatatacaat ttgtagctaa agaggataag
     6181 gcaatagttt ataaaactat agaagataat ctctatgtaa aagaatacat aaatattagt
     6241 aaaaaagaaa tagaaatgaa aaatacatca aacaatgatg cagtagaaaa taaattaaaa
     6301 gataataata aagtaagtaa aagttatcaa aataactata cagaagatga gatacaagaa
     6361 gatgttttaa atgaaaaaac tgatatggaa aaagatagtc taagtgaaga aaatgaacat
     6421 ttaccaataa gtaagcagag tattatgagt gtaaatttag aaaaattgga cttacttcaa
     6481 aatatagttg gagaaattgt aattatggaa tcaatggttg taaatagcgt aaattctaca
     6541 ggtgttgata tagataattt caataaggca acaagacaat tacacaagct tacagatgaa
     6601 ttgcaagata tagtaatgtc tatgcgtatg attcaaattg ctggtgtatt tcaaaaaatg
     6661 catagaattg tacgagatat gaataaaaaa ttagataaaa atgtagagtt aataactata
     6721 ggagcagaga cagaggtaga taagagtgta attgataatt tagcagaacc ttttatgcac
     6781 cttataagaa atgctatgga ccatgcaata gagcctattg aggagcgtat agcgaaaggt
     6841 aaagaagaag tgggaaagat agttttatct gctgaaaatc taggcggaga agctgtgata
     6901 agtatttcag atgatggtag aggcattatg agagataaga ttttggcaaa agctaaggaa
     6961 caaggtcttc ttaaacatca aagtaatgag tatactgatg acgaggtcaa ttctttgatt
     7021 atgactccag gcttttctac aaaagaatct ataactgaat tttctggaag aggagtaggc
     7081 atggacattg ttcgtaaaag tattgaaaaa gttggaggtt caattgaagt aaaaagtaaa
     7141 caggatgaag ggacaacctt tactataaaa ataccattaa ctctttctat agtagagggt
     7201 atgaagttta aagttggaga ttcactattt actttaccaa tatcatccat aagaagaaca
     7261 tttaaactta cagacttaaa tcaaataatt acagaaggaa ataagacaga gttaattaca
     7321 atatatggag cctgtattcc cattataagg ttacataaaa tatataatat tcaaacaaaa
     7381 gtaaccaatc tgatgaatgg taatatgata attattgaaa atgagcataa agcagtatgc
     7441 atttttgttg atgaaataat aggtaatcag caagttgttg tgaaaccatt tccagtttat
     7501 tttaaccgat ttaatataaa aaataagggt ttatcgggat gtacaatact tggggatgga
     7561 agtataagtt taattataga tgcagatagc attatagaaa tatattaggg gatttaaagt
     7621 atggctaaag atgaaattat taaacaagta ttaacatttt atgtcaatga tgttatttat
     7681 ggaatagaat tagaaaatgt gattgaaacc ataagatttc aatcaataac gtatgtacca
     7741 tgtcttccaa catatataag tggagttatt aatttgagag gaagaataat accagttatt
     7801 aatatgcata taaagtacaa tcttcctaaa gctgattata atgagagaac ttgtataata
     7861 attacaaagg ttgatgagta tcaagttgga attattgtag ataaagttgt tgatgttatt
     7921 catatagaaa atctaaattt actagagacg actaattcta acaacactaa tattaataaa
     7981 gatataaaag agattgcgaa agttgaagat aattctatat taattttaga tattcgaaag
     8041 tttttgatta atagcatgta gttagttctg ataaattagc aggaggaagt ttttgcatga
     8101 taaaactcac agatgaagaa tttatagttc ttgtaaacca tgtaaaaaaa gaatatggta
     8161 ttgatttaag taaaaaaaga gcgttaattg aaggtcgctt atacaataca atgatagaga
     8221 aaaaacttag ttcttttagt caatatatga atctattatt taaagataag acaggaaatg
     8281 aagctataaa cctaataaat agattgagta ctaaccacac attttttatg agagaacctc
     8341 aacattttga gtttatacag aatagtatac ttcctttttg ggaagaaaat aataaaataa
     8401 gaaacataaa tatatggagt gcaggatgtt ctagtggaga agaagcgtat tcaatagcta
     8461 tgactttgga tgattatttt ggatataata aagagttgtg gaacattaaa atcatagcca
     8521 cagatatttc aactaactca cttgaaaaag ctaaaagagg tatatacata gaatcaaatg
     8581 ttaacaatgt tcctgagtta tggaagaaga agtattttat agacaacaag gatggtacat
     8641 ttaaaatttg tgataagatt agaaaaagtg taatttttaa gccgtttaat ttaatgaatg
     8701 atttctcata tcaacatttt gatttgatct tttgtagaaa tgttatgatt tattttgatt
     8761 taaaaacaag agaagaactt ataaataaat tttataatgc taccaaaaaa gatagttttt
     8821 tgtttgtagg acatgcagaa gtaatcaaca gaagtagtac taaatacaaa tatgtaaaac
     8881 ctgctatata taaacgataa aaaggtattt ttgatatatg attttttgga ggtggtaatg
     8941 tggaacgttt taataatatg gttattgcta taggcgcatc tgtaggtggt acagaggcaa
     9001 tacttgaaat tataaaagac ctaccaaaat caacaccagg gattgttgtg gttcaacata
     9061 tgccagctat atttacttat atgtatgcac aacggcttga caagcagtgt attatgaatg
     9121 taagagaagc taagaataat gatagagtcg aacaaggaaa tgtgttaatt gcacctgggg
     9181 gttaccagat gaaattgtgt acggataaac aaggatatta tgtaacttgt gaaaagggag
     9241 aaagaataag tggtcactgt ccatcagtag acgttttgtt tgattcagta gcagaagtag
     9301 caggtaaaaa ttcaatagga ataatactta caggtatggg ttatgatgga gcaagtggac
     9361 ttttaaaaat gaaagaaaat ggttcattta caattggaca ggatgaaaat tcttgtatag
     9421 tatatggaat gccaatggta gcttttgata gaggttctgt tatgttacaa ttacctcttg
     9481 gtgaaatttc aaactgtttg ataagacata ttaatatttt aaaacaaaaa gattaatttt
     9541 ttataaataa aaattaatct tttattaaat tatatggata aaaaacccta gaaactccaa
     9601 atataataag agagatttat atttaggagg aaaatgataa tatgaaattt tttaaagtcg
     9661 atacaaaagc taaagtttta agatatgtat ttgttttaac attgactata atggctgttt
     9721 tggcaattaa acatgactat aaatataata atttaaggaa agatgcttca attaatttaa
     9781 atagtaataa ggaagcagtg actgttatga gtaaccagtc attaccaaag ggtaatacaa
     9841 atagtaaaca aactttatca gtaagtacaa taggaagtaa aacaatagtg aaaaatgatg
     9901 agtatttaga aagtactata aacatgcctg taataacaaa ttcaaataaa attgttgaaa
     9961 ggtctacaaa tgataagata aaaaatgaca tatttgaatt ttataataaa tcatataaag
    10021 aagcaaagca gtatcttaag tacaatcctg acgagaaaaa taagtttgtt gcaaatgtag
    10081 actttgaact taaaaagaat actgattctg ctcttagtat aaaagtgaga tattatactt
    10141 atagtggtgg agcacatgga ttttatcaag atatagcata taatgtggat atgagaactg
    10201 gaaaattttt agaattaatg gacttgttta aggataatac taaatataaa gaagttatag
    10261 atgaagaaat taaaagacaa atagctgaac ttgaaaaaaa ggatgaagaa aatatcggaa
    10321 tatataattt taaaggtata aaagagaatc aaaactttta cttgcaagat gagaatctag
    10381 ttgtatattt tgatttgtat gatataactc catatgcagc aggaatacca gaattttcaa
    10441 taaataaagc gttgataagt tctatgctaa aaccagagta tgtagattta tttgatttga
    10501 aatagaagat taaattaaat gttatgctat aatatttagc atagagactt attaaaattg
    10561 ggggtataaa aatgctactt ttaaaaaaag acgacataaa aaaagtattt acaatgagag
    10621 atgcaattga agctgataag gaagctttta gaatttattg tgaaggaaag agtgtaaatc
    10681 ctttgagaac caatatatct gttccaagtc aagatgctag tatgttattt atgccaggat
    10741 acgtagaaga attaggctgt gggggaataa aaattgtatc tgtatttcct aaaaatgctc
    10801 aaaaaggcaa accagtaatt ccagcaacag tactattatt agatggagaa actggagaag
    10861 ttagtgcagt attggatggt acttatgtaa ctcagattcg tactggagca gcctctggag
    10921 cagctataga tgtattggct aaaaaagatt ctaagattgg agctttgata ggtctaggag
    10981 gacaaggtga gccacaactt gaagccatga tagaagctag aaatttggat gaggtaagag
    11041 tttttagtag aaacaaggaa agtagagaaa aatttgcaga agaaatgaac ttaaaattaa
    11101 gtaaatataa tactaaaata gttgcagttg aatcatctga tgaagccata gataacgcag
    11161 atgtgatagt tttagctact ccatcaaaac aaccagtact taatggaaac ttagtgaaaa
    11221 aaggagcttt aataagtgca gtaggttcat atatgccaga tatgcaagag ttgtgcccaa
    11281 actgtttaac tagagcgtct aagatatttt ttgaatcaac agatgcagtt ttatctgagt
    11341 caggagatat attaattcca ttaaaagaag gtaagataag taaagatgat tttagtggtg
    11401 atttagggaa tgttataaac ggaactattc ctggaagaga agatgatgat gagataattg
    11461 tatttaaaac tgttgggata ggtgtacaag atgtcgtaac agcaaaaaga atatacgata
    11521 aagcaaaaga aaacggtatt ggaatagact ggaagtggta aaatattcaa aataattcta
    11581 tagttagaca aatttatgtt ataatgttga taaataaatt ttttattatt ttaattgagg
    11641 gaggatgttt gtatgaaaat ggtaaaaaag ttaatattat ctacgattat ggctctagtt
    11701 ttagttctaa ctatagggtg ttctaatgca gaagaaaaag taaagtcgaa ttttgaagct
    11761 aatttagctg cattagaaat gtataagtat gatgaaaaag gaatttctga tgcattaaca
    11821 gaagctgaat ataaacagat tcaaacttat aacagcaagg acaaggtttc aatgattgct
    11881 caattgacac ctctttatgg taaagaaaat gctgaaaaga tatacgaaac aattgttaaa
    11941 ttaattcaag gtgttgaaaa gacagtaaca gtggaatctt ctagtaagga taagataact
    12001 ttgaaagttg taagtaaagc tttagatact agtaaagtaa tgagtgaagc gaacacttta
    12061 gtaaaatctt atgtaactga tgaaaagaat tacgataaaa aagaaaaaga agttcaagat
    12121 gatatgacaa aaatgattat agacctttac aataagaaac caactaagga agttacaact
    12181 atgattacat atactaaaaa agatggtaaa tggcaaccac ctagtaatat acaaaagtta
    12241 tgtgaaggaa tatatacaat ttaatttatt ttaaagacac aaaactatct tttaagcata
    12301 taataaaatg tttggaagat agtttat
//
LOCUS       DACXMZ010000108        12205 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.109, whole genome shotgun sequence.
ACCESSION   DACXMZ010000108 DACXMZ010000000
VERSION     DACXMZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.109"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            85..975
                     /locus_tag="JD016_11515"
     CDS             85..975
                     /locus_tag="JD016_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAU5000261.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            1193..2479
                     /locus_tag="JD016_11520"
     CDS             1193..2479
                     /locus_tag="JD016_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAU5000262.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(2568..3011)
                     /locus_tag="JD016_11525"
     CDS             complement(2568..3011)
                     /locus_tag="JD016_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000263.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(3035..3829)
                     /locus_tag="JD016_11530"
     CDS             complement(3035..3829)
                     /locus_tag="JD016_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid amidase"
                     /protein_id="HAU5000264.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(4395..4871)
                     /locus_tag="JD016_11535"
     CDS             complement(4395..4871)
                     /locus_tag="JD016_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAU5000265.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(4972..6150)
                     /locus_tag="JD016_11540"
     CDS             complement(4972..6150)
                     /locus_tag="JD016_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAU5000266.1"
                     /translation="MCFMLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIK
                     YLNEIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSTNIGKM
                     HACGHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNV
                     KYIFGLHVNPNINKNLIELKCNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHI
                     ITALQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKR
                     IREICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTL
                     GAEDFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVILHVKNVLSF
                     "
     gene            6304..7167
                     /locus_tag="JD016_11545"
     CDS             6304..7167
                     /locus_tag="JD016_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAU5000267.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            7238..7831
                     /locus_tag="JD016_11550"
     CDS             7238..7831
                     /locus_tag="JD016_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HAU5000268.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(8081..8716)
                     /locus_tag="JD016_11555"
     CDS             complement(8081..8716)
                     /locus_tag="JD016_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAU5000269.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(8796..9527)
                     /locus_tag="JD016_11560"
     CDS             complement(8796..9527)
                     /locus_tag="JD016_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAU5000270.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            9858..12047
                     /locus_tag="JD016_11565"
     CDS             9858..12047
                     /locus_tag="JD016_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HAU5000271.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEIPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT         4691 a         1401 c         1898 g         4215 t
ORIGIN      
        1 aatatttaga taattattat ttaatgtata agctaagttt ttaattaata tggctttata
       61 catttatata gaaaagaggt acgtatgagt agcacaatca aatcaaatcg aataaaaggt
      121 ataatgttta taatagcatc agcgcttggc tttgcaatga tgtctgcatt cgtaaagcta
      181 gctggagatt taccaagttt tcaaaaagtg ttctttagaa atctggtatc agctataata
      241 gctttttggc ttatcataaa acataaagga agtctaactg gtaaaaaaga aaatttaaaa
      301 gtgcttttat ataggtcaat atttggaact ctaggagtta tatttaacta ctatgcaatt
      361 gacaagttga tgctttcaga tgctaatatg ctaaataaaa tgagtccctt ccttgtagta
      421 atattttgtg caattgctct aaaagaaaaa ataaatgtaa agcagatagg agctattata
      481 attgcattta taggagcttt attcataata aaaccaacat ttagtgtaga agtaattcca
      541 tatcttggag gaattgcagg agctgtattt gcagcaatgg catatacttg tttaagagtt
      601 cttggagata gagaagatta ctatacaatt gtatttttct tttcagtttt ttcattagtt
      661 actgtagggc caattgcttt tgcagtatat gaaccaatga cccttatgca acttatatat
      721 ctattactag gtggagtatt tgcaagtcta ggtcaatttg gaataactct agcatataaa
      781 tatgcacctg ctaaggaaat ctctatattt gactatagta atataatctt ttcagcaatt
      841 ttaagtatat tcttattcaa tgtctatcca gatatgttaa gtgttgttgg atatttaata
      901 gtattttcgg cagcatttta tatgttttta tacaataaga agttagataa attagacaaa
      961 gaaaagaaga agtaaactag ataaaagtgt ttttataatt tgtattgaat atttaagtct
     1021 ttgtattaaa attaaagaaa atttaattaa aaatgtttgg tatattaact tataacagca
     1081 gttaaaattg aaaaattata actgctgttt cttttattaa aaatatccaa atgtctgtaa
     1141 aaatagacta taatagagta gtataaataa tataaagggg ataaattaaa gtatggaaaa
     1201 agtgaaagtt gctgtgattg gagcaggtaa tagaggaacg tatgcatatg ctccatatat
     1261 ttatgaaaat tctgatgtat gtgaaatagt tgctgtagca gaacctaaaa aaggaagacg
     1321 agaactattt acacaaaaat ataacctaga tagtaaaaat gtatttgaaa ctttagaaga
     1381 cttttttaaa catgacaaga tggcagatgc agtaataata gctacaaatg atgatagaca
     1441 ttatgatgta gcaaaactag ctcttgaaaa aggttatcat gtactattag aaaagccaat
     1501 gtctaatagt ttagatggtt tggtacatat agatgacttg tgtgataagt ataaagataa
     1561 gatttttatg atttgccatg tactgagata ttctccattt tataataaat taaaagaaat
     1621 tgtagaaagc aaaaaattag gagagctagt tagtatacaa tataatgaaa atataggata
     1681 ttggcatttt gcacatagct ttactagagg gaattggaga aatagcaatg aaaccagtcc
     1741 tttaatttta gcaaagagtt gtcatgacat ggatattttg ttatatttag taggaagtag
     1801 atgcaaaaaa atatctgcat ttggaagctt aaagcatctt aataaccaaa atgctagtgg
     1861 agagatggca caaaattgtc ttcagtgttt agttgaaaaa aaatgtccat attcagcaaa
     1921 gagaatttat ttagaaaaag atagaagtat aaatagagct gttcacataa atccaactga
     1981 agagaatcta ttaaatatac ttaagacaag tccatatgga agatgtgttt acagatgtga
     2041 taataatgtt gtagacaata tggtaaatat actacagttt gaaaatggag tgacggctac
     2101 ttttaattta tgtgcattta ctaaagaaaa tggaagaact ataaagttaa tgtttagcca
     2161 tggtgaagtt ggtggggatt taaataaaaa tgagataaga attaaagaat ttggaaaaaa
     2221 cgaagaaata gttatgaatc catcaaatca acagaatatg gtagaggaat atgatagaaa
     2281 tcttatagct gaatttatta aattagtttc aaataaagaa ctagaaaagg gtagagtagc
     2341 agcaaaagaa gctatacaaa gtcatgtaat ggcatttgca gcagaatatt ctagggtcag
     2401 tgatgaagta gtgtatatag aagaattttt tgatagtgct aagcaaatga ctaaggagat
     2461 agaagaaact atattttaat taataaaaat ttattaaata aaataaagat agattaaata
     2521 atagaaatac gaaaatatgt gttctattaa ggaatctatc ttttttatta aggttttttc
     2581 ttatccctat caaaaaaatc catacattct gataaaagat aagaaagtaa agcgaataag
     2641 atttctgtac ttaaaggaat cgttactgaa tccataaagg tatcaattat gttcatagct
     2701 agaaaagtta gtataaaatc aattataaat tcgcaaagtt tatatattga gtatggtaat
     2761 ttgtttacat atctgaatat atctaaaata gtagtgcata gaaaatcaat tggagtggtt
     2821 atacaaaaat atattaaaaa gaaaattaat acagctccaa gagatttata ttcaaatcct
     2881 aagaatgata agaagccaat tcctataacc gtgaaaatac aaaaagttat accaaaaaaa
     2941 acagcaaaaa ataaaaatac agaaataaat tcgcctaaag gaatccttct tttattactt
     3001 atcttaatca actccttaat ttatgatatg aaatttatat aaataataac attcttaaaa
     3061 attcataata gtcctctgtt tcaaatagta ctgtttgaga atctattttt ttcatatttg
     3121 gataaaaaga ggccttaaaa gctttataat gttctctaaa ttttatttct atcttaaatt
     3181 tatctggcaa ttttattaag tgcctagata aatctccaga taaagctttt ttgacacctt
     3241 tttcaatttt ttttagtgca aggtttggat ggattgaaat tgaaccattt ccaattcctt
     3301 ttgatacagg aacagtgact atatttggat ttagtttttt tgcactctca caaagcattt
     3361 catctccact taaaaatgct acaggaacat tataatatgc agcaatataa gcatttatag
     3421 taaattcaga tgctatttca tcatttattt taaaatagtc ataatcaata tccatagtat
     3481 gggaaagtgg attaccatta caagtagctc cagagtgata gccagtgaat atacatgcat
     3541 caaagctttc atcaattcca gccatcataa ctagtgggtc tttggtccat cctctaagta
     3601 ttttgacgtt ttcaggtaaa atatttggat taatatttct accactgtca tgtgcatctt
     3661 taacaagtat ttcatgtaca ccagattgag tagctccatc acatgctgct gttatctctt
     3721 tactcatttg tgttctatag tgttgtgaaa attcacattc aagttttgtt tcatcccaat
     3781 ttacaatacc acaagtacct tctatatcag cacttaaata caatttcata atatataccc
     3841 ccttatgttt attattgatg ataataacag ataacagaaa atacataatc aataaagggc
     3901 ataaaaatat tatgtcttac tattaattat tattaaatct gtaactgaaa aataactaaa
     3961 ttggtattaa aaaatataac actatgtgtc atttatttat atagtttttt atatctgatg
     4021 tttatataaa attgtaacat ataagtggtt acatttacta gtttaacata gttaaaatag
     4081 ttaggtatta tttttaagtt aaattgatta ttataaaatc gcagaataaa agaaaaattt
     4141 aatttattaa aatgcatgaa atgttttttc ctcaacataa tgctaaatta aactcaaaaa
     4201 gaaataatag aaaaatctta tgtagcttag aaagaatgtg aatgatttaa gggaaaaata
     4261 gaaatttatt atataatatt aggctgtatt atataataca gcctaatatt atatgtatat
     4321 gtgacataaa atcttgctaa gtgtaaataa aataaatgtg gaaaacaagt taatgaaatc
     4381 aagaaaaaag taatttaaaa ttcaatttca tcttcccaat taaattctcc tgatttttct
     4441 acactcttag gccagtgact acaccattta ggaacggacg gattttcaac cctatcaacg
     4501 cttggagtat atggctttaa gtcaataaca ggactattat cattagcatc aatatatggt
     4561 atttttatta tgccattttc atggtcgata ttgataattt gtacagcagt caatgctatt
     4621 ggattgggtc ttataggtga ccttgttgca aatattccta taacgtctgg tccactttta
     4681 taaggttttg gggtttctaa aatacttctt agttcaggat tatcaaaccc atcagcccac
     4741 caaaatacat ttaaatgact gaaatcgtcg agttcattta atgcaggtac atactttttg
     4801 tctaatttaa taaagacctc ttcgccttga atacatattt tacctatagg atttattaat
     4861 atttctttca taatattacc tccaactatt taatttatca tttgtaattt tagggtataa
     4921 catgacatca tgtgagggtc aatataaaat aataaataaa aatattaata gttagaatga
     4981 taatacattt tttacatgga gtatgacacc agtaataagg caatcctcgt caatatcaaa
     5041 agtagaggta tgcaaagggg aagtaatgtt tttttcacga tttgcacaac caaggtggta
     5101 aaaagctcct tcacagtgtc ttagaaaata cgaaaagtct tcagccccaa gagtgggcga
     5161 gtctttaagg ataatatttt cttctccaaa caattctaca gcattttgtt tgacgtaatc
     5221 tactagatgg tttgaattga taagagctgg ataaccttcc tcaatttcaa cagaaatact
     5281 tcctccaaat gttttacaag taaaatcgca aatttctcta attcgttttt ttgaaaactc
     5341 tctagtttca ggagtcaagg ttctcaaagt accccttata accactttgt cacaaactat
     5401 attttccttg attccacctt caatctttcc tagagaaata accacagaat cagttggatt
     5461 tgtgtttcga ctaacgattg tctgtagagc tgtaataata tgagcagata taacaatagc
     5521 atcaatacct tgatgtggat aagctccatg acattttgaa ccatgtacag ttaattgaag
     5581 tgtatcggtg gaagcattta gagtgttgca ttttaattca attagatttt tgttgatatt
     5641 gggatttaca tgaagtccaa atatatattt tacatttggg ttttcaagac atccatcttc
     5701 tatcataagt tgagccccac caatagtttc ctcggcaggt tgaaaaaaga attttacatt
     5761 tacatttaag taatccttta atttaaatag tatattacaa gtacccagta aaatagcagt
     5821 gtgtgcatca tgaccacatg catgcatttt gccaatatta gtagatttat aagaataatt
     5881 aagttcttct gttataggta aggcatctat atcagctctt atagcaactg tttcaaagtt
     5941 tggactcacg ttaatgtatg cagttatccc tgtatgattc ttatattcaa tataatttat
     6001 acctatttca tttaagtatt taataatttt tttcttagtt tgaaattctt ctaatccaag
     6061 ttctggagtc ttatgtaaat ctcttcttat atttataacc caatcattta tctcatgaca
     6121 aagagttttt atattaagca taaaacacat cctaactata ttaaaaattt atttttcttc
     6181 attaattata ttattttatg taaatcatta ttatgtatca agtacaaata aaatatctta
     6241 taattatatt agagatattt agtatggaga agaatatatc attttataag aagaggtgtt
     6301 taaatgttag gaataattgc gccatctggg ccattaagaa atacaagttt ggaggaaata
     6361 aagtttaatt tagaaagtta tggttacgaa gttaagtttt ctgaaagttg tagtttaaat
     6421 tataaaggtt atttagctgg taatgatgat attagagcaa gggatataga agatatgttt
     6481 ttagataaag atgtagatat aattatgtgc ttaagaggag gttatggaac aacaagaata
     6541 ttggataaaa taaattatga tataataaag caaaatccaa aaccatttat aggtttttct
     6601 gatataacag gtttaaattt agctttctat aaaaattgtg gtcttttacc atatcatgga
     6661 ataatggcag caaatgtagg taaatgggat aaatttacct ataaatcatt ggtaaatgct
     6721 ttagaattta aagatgaatt atatttagaa aatccaaaag aagaaaagat ttatactgta
     6781 tgtgaaggta aagcagaggg aataataatg ggaggaaatt tatccttaat aattgccaca
     6841 atgggaacaa aatatgaaat agatgcaaaa gataaaattt tgtttattga ggaaattgga
     6901 gaacctcaat ataaattgga tagaatgtta acacaactat attcttcagg aaaacttgag
     6961 gaatgtaatg gtataatttt tggagatttt aaggattgta ttgaagaaaa tgatttgatg
     7021 gaattactaa tagaatttgc aaataaggtc aataaacctt ctatatataa tcttcagtca
     7081 ggacattgta tcccaatgat aactattcct ttgggcagaa tgtgcgaatt agatgctaca
     7141 gaaaaaatcg ttaaattaaa aaaatagtgt gtatataaat ataatagaaa tatggtataa
     7201 tcttatacaa gtaaaaattt agctaggagg atgaaaaatg tctgatgata gaataataga
     7261 ctttaacgaa ctaaagaaca aagttaaaga ttctgatgta gataaatttg agcaatacat
     7321 atataactta tatttttctg ttatggatgg aaagatgtct atggctgagt tttcaagaaa
     7381 aatattcgat tatatgagag ataacaacat atctcaagaa aaatttatga aaatacaaaa
     7441 gcaatttatg gagagatatg gtatggacac tgaagaagta gaaaaacaac ttagaaactt
     7501 tggaatcgac ccttctacag caggttttat gagtaataat acttccagca aagtatctac
     7561 agaagattta gagtcattta agaagagtgc aggattttat gagaaatatg gagaaaaaat
     7621 ccaaccaaag agttgtataa caacatttat aaaaaatgac ctaaatgata ttaatgtaat
     7681 aatcgaccaa gaaaagataa tgctttgtag tgatagaaaa ataaatctta tggattcaga
     7741 actaaatgag tttttattag aatataagaa tatgtttaat aaaaaaataa aggtagtaat
     7801 gtgcgaaact acgaataagt atgattatta aaaaatgaat ttaaatactg aagtagtatc
     7861 aattaattgt actacttaaa aataaaaatc gcaatttatt attccttata aatttgtata
     7921 aggaatatct aaactgcgat tttttatatt gtgaagatta gtcataatag atagtgtttt
     7981 aaattgtaat cttctcacta aaactgtgtt tttctattag aaattttatt aaaaataaat
     8041 atctgataat tgtattatat ggatgtaaag tattagaggc ttaacgaatc tcttttaata
     8101 cattaactcc attttgaatc atactgttga aaaatactaa gtttaaaatc tcagcagggt
     8161 gaacgttagg aatatcataa gtatctacta aattcatagg tacaactata tttttttcaa
     8221 tattatgttg attaaaatat gattttagag ttattgcaaa ttgatatata catatatctg
     8281 tgcaatcgcc aactataatt atattatcta tattatcaaa atccaaattt tctaatgcaa
     8341 aaaatccatt agtagaattt ttaggaagaa tttgtaggtt ttctatcgac ttaagttcat
     8401 caaccaattc actttctaca tcattttcca aacagtgaac aggataactc aaaagttcta
     8461 ttgatttagg agtatgagaa tcagtaaatg ctattactct atttaatttg tttgatattt
     8521 tttttgtaaa catttcaatt ggctttatta aagactctac tctaggagag tataatgcac
     8581 cttgtcttgc aaagccatta ttaacatcaa ttataaataa agcagtttta ctcaaatcat
     8641 aattttctat tttttctatt ggtagattat ctaaattact ctttaaagct tcaagttcat
     8701 ttaataagtt atccatagtt atacctccaa gataaattta ataaaattgt acatattaat
     8761 tatataaaag tcaaggagta caaactattg tataattagc tgtattgttt tgagtgtaaa
     8821 agatttgaag caatatctgg atagttagta aaaataccat ctacattgag ttttagacag
     8881 tgtagtatat catcctcttc atttacagta tatacattga caccaagtag atttttgtga
     8941 caaagttcaa ttagagattc atttactaaa actagaggtg gatgaagata gctattaccc
     9001 agaaatttag caaaattgac tacattgttt ataggtaaat aacataataa tcctgttttt
     9061 atactagggt ctaatttatg aaagtttttt aaactattgc tgttgaaact agatataaga
     9121 actctatttt tcatattata ctttaaaata agctctaaaa cgtctttttc tatattggga
     9181 tagtttattt tgtcagtttt tagttctata tttaaaataa ggtctgtagg tgaaattaga
     9241 attaatactt cttctaaagt tggaatttta cattctttat tgctttgata tccttcaaaa
     9301 gatgagttta gagtttttaa ttttcttaaa gtaaagtctt ttacaaaacc atgtccatta
     9361 aaagttctat caaccttttc atcatgaata actacaattt ttccatcctt agttctgtgg
     9421 acatcaagtt ctattccata tatatccata tccaaacatt ttttaaaagc tagaatagta
     9481 ttttctggat actttccact aaaccctcta tgagcataaa tattcattta tatggttccc
     9541 tcctataagt ataacttatc ttataaaaat ttaattaata taatatatca tattccaaaa
     9601 ggtattgtta tatattagtt aaataactat ttgtaattta tatattatat aaatgtagaa
     9661 aaataaaatc atatatgtgt tataatatat tttaaacatt gtaaatatta cgaatttagt
     9721 agtcataata agtgtaattt tatgcaagct catgttttta gagcttgcat atgtattatt
     9781 aatatattta tatattgtga aaataaaatt ccaaaagaat tttttctata gacttagtag
     9841 taagggggaa ttttgatatg tatgaagtta atttagaacg agctatgaat tattggatgg
     9901 atttgttaga tgattatacg aatatagcaa aaattccaaa tgattttttt tataaagatt
     9961 gttctgatga agtagtagat gaggttttta agatgaatat tgagaaacat gaaaggttaa
    10021 ttcaatattc taaagtgaac aatattagca tagactctat catagaaact atatatggcc
    10081 ttatacttca aaagtatata tatgaagatg atgtagtttt tggtaaaaca atgaatataa
    10141 ttccaataag agtaagagtt agacaaagtg aaaaatttat agatgttgta aggaagctaa
    10201 aaaggcagtg gagcaggtct ttagagtatg gatactattt gttatcagat atagagaata
    10261 aaaatcatct aaatactaag cttataaaca caatatttat agctaaaagt tttgatttat
    10321 catatgatta tgacatagaa aaaaaaggat atgatttatc tgtaatagta tatactaaga
    10381 gggcacttaa aataaaaata aagtataaac catctatata taaaagagaa actattagaa
    10441 gaattttaaa tcaatttgat tatataatag agcaaataat agaagataat gatattttaa
    10501 tagaagattt agaatttgta tgtaaggaag aagaaaaagt attattggaa gactttaata
    10561 ataatcaata tgaaatacca tatgataaaa gttatgttga tttatttagg gagcaagtta
    10621 aggtaacacc tagtaatata gctgctaggg atgaaaatag aaagttaaca tataaagaat
    10681 tggatgaagt aactgataaa cttgcaggtt atttaaatca tataggagtg gaaagtgaag
    10741 acatagtagc tgtaatgtta ccaagagata ttaacataat tattacagca atagggatta
    10801 tgaaatcagg gggagcattt tttccaatag acacaagtaa tccagaagaa agattgaatt
    10861 accttcttga agatagtaat gcaaaagtag tgataactac agatgaactc aaaagtaagg
    10921 ttgtaaatga gaataccata gtactcgata taaatgatga agaaatgttt aagcttggat
    10981 atgaattaac tgaaaaaata acatcatcaa attgtgcata tacgatatct acttcagggt
    11041 caactggtag gccaaagacc atagctatag aacataagag tttagtaaat atgtgttatt
    11101 atagcgtaaa atctataagc gctacagaaa atgatatatg tggtatatac ttaagtttca
    11161 gttttgatgc tgtaatgaaa caactattcc cttatttgct tgtaggtgca tcaatagata
    11221 ttatgcctga agaagctaaa tttgatgaat atacagtaaa tgaatactgt gaggaaaatg
    11281 acataactat actagcattg ccaacagcat ttgcaaggtt atttatacaa aattgcaata
    11341 ataattcact tagagttgta cagaccggag gagaaagatt aaaaggatat agaaagcgta
    11401 attatgaact atataatgag tatggaccaa ccgaatttac tgttgtatcg acttcttttc
    11461 atgtggacag agaatatgga aaaataccaa taggaaaacc tatatttaat acttatgcat
    11521 atgtattaga taaaaagaac aaactatgtc caattggagc accaggagag ctatgtctat
    11581 caggcattca aatatcaaga ggatatttaa ataaaaaagg gctaacagag caagtatttg
    11641 tagagaatcc ttacaaaact tgtgaacata ataagttaat gtatagaact ggtgatttag
    11701 taagatggtt agatgatgga aatttagatt atattggaag gatggacaat caagtaaaaa
    11761 ttgatgagtt taggatagag ctatatgaga ttgaaaatat aataaataat attacagaaa
    11821 ttaaaagtgt tgtttgtata tcgagaacaa atgatgatgg agatatgtat atatgtgcat
    11881 attatgtaat tgatgaggaa gatagtggta agataaatga aagaactatt agagaatatt
    11941 taaatgagca tctacctcca tatatgattc caacaataat aatgaggata gataagatac
    12001 cagttacacc aatcggaaaa gtaaataaaa gagcattacc agaataattt tttattatga
    12061 taggtgtcat aaagtgattt cagacatttc tttatgacac ttatttttat aaaagaatca
    12121 atttgcataa aaacaagaat tttctgtata tatttactat ggtgaaaatt tatttataag
    12181 aatgattatt gtatttttta tactt
//
LOCUS       DACXMZ010000109        12174 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.110, whole genome shotgun sequence.
ACCESSION   DACXMZ010000109 DACXMZ010000000
VERSION     DACXMZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.110"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1699
                     /locus_tag="JD016_11570"
     CDS             <1..1699
                     /locus_tag="JD016_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAU5000272.1"
                     /translation="LAPGADLSGKVLSLGEGFSMAFSNPLPSLAPMDLLIGILGAVLI
                     RLIVYVKGKNAKKYRKGIEYGSARWGTAEDIKPYTDPVFQNNVLLTQTERLTMNSRPK
                     QPKYARNKNILVIGGSGSGKTRFFVKPNLMQMHSSYVVTDPKGTVLVECGKLLQRGGY
                     RIKVLNTINFKKSMKYNPFAYLRSEKDILKLVNTLIANTKGDGEKAGEDFWVKSERLF
                     YCALIGYIWYEAPEEEKNFTTLLEMINASEAREDDPEFQSPVDLMFERLEEKDPEHFA
                     VRQYKKFLLSAGKTRSSILISCGARLAPFDIKELRDLMETDEMELDTIGDRKTALFVI
                     ISDTDDTFNFVVSILYTQLFNLLCDKADDEYGGRLPVHVRCLLDEFANIGQIPKFEKL
                     IATIRSREISASIILQSQSQLKAIYKDNADTIVGNCDTTLFLGGKEKTTLKEMSEILG
                     KETIDSFNTSENRGREVSHGLNYQKLGKQLMTEDEIAVMDGGKCILQLRGVRPFFSDK
                     YDITKHPNYKYLSDYDKKNTFDMEKHLRRRPALVKSDEPFDYYEISEADLQEDTAHE"
     gene            2074..2289
                     /locus_tag="JD016_11575"
     CDS             2074..2289
                     /locus_tag="JD016_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU5000273.1"
                     /translation="MEFFNSAIDVLQTLVIALGAGLGIWGVINLLEGYGNDNPGAKSQ
                     GIKQLMAGGGVALIGTILVPLLSGLFG"
     gene            2308..3177
                     /locus_tag="JD016_11580"
     CDS             2308..3177
                     /locus_tag="JD016_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000274.1"
                     /translation="MESILEAINEWIKEILIGAINGNLSTMFGDVNEKVGTIAAEVGK
                     TPQGWNANIFNMIQNLSENVIVPIAGLVITYVLCYELISMVTEKNNMHDIETFMFFKW
                     FFKAFVAVFLVTHTFDITMAVFDMAQHIVSGAAGVIGGDTNIDVTEALAAMQEGLKDM
                     EIPELLLLVMETSLVSLCMKIMSVLITVILYGRMIEIYAYCSVSPIPFATMTNREWGQ
                     IGNNYLKGLFALGFQGFLIMICVGIYAVLVGEMVLADNLHSAIFSLAAYTVILCFSLF
                     KSGALAKSIFNAH"
     gene            3220..3624
                     /locus_tag="JD016_11585"
     CDS             3220..3624
                     /locus_tag="JD016_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAU5000275.1"
                     /translation="MAYVPVPKDLSKVKTKVAFNLTKRQILCFSMALLMGLPLFFLLK
                     DSAGTSLAAMAMIVVMLPCFLVAMYEKHGQPLEVVVKNIIQTKFTRPKVRPYQTENLY
                     ALLEKQRALEKEVSAIVKRTDKKDAGNRRKQA"
     gene            3572..6001
                     /locus_tag="JD016_11590"
     CDS             3572..6001
                     /locus_tag="JD016_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAU5000276.1"
                     /translation="MSKGQTKKTRETAGNRRKLTRAEKKQIAAVIRQAKGDGKAHTAQ
                     QTIPYLAMYPDGICKMAQRRYSKSIAFEDINYQLAQADDKTAIFENWCDFLNYFDASV
                     NVQLSFINQGSQQEQAARAIHIPPQNDDFNSIRTEYSDMLKTQLAKGNNGLVKHKYIT
                     FSIEADNPAAARARLSRIETDILNNFKVLGVSAHPLSGYERLKVLHGVFHPAGEPFSF
                     SYDWLTPTGLTTKDFIAPSSFKFGEGRYFAMGKKTGAVSFLEILAPELNDRILADMLD
                     LETGVIVNLHIRSIDQAEAIKTIKRKITDLDKMKIEEQKKAVRSGYDMDIIPSDLATF
                     GNEAKNLLQDLQSRNERMFLLTFLVVNMADTKRKLENDIFAAAGIAQKYNCALTRLDY
                     QQEAGLMSSIPLGENLIPIQRGLTTSSTAIFIPFITQELFQTGAALYYGLNALSNNMI
                     LCDRKQLKNPNGLILGTPGSGKSFAAKREITNAFLITDDDIFICDPEAEYFALVKRLG
                     GQVIRLSPTGKGMDGTPQYVNPMDMNLNYSEDDSPLALKSDFILSLCELVIGGKEGLQ
                     PVDKTVIDRAVRNVYQDYLADPVPEKMPILGDLYNELLKQPEPEAARIAAALELYVSG
                     SLNVFNHRTNVELSNRLVCFDIKQLGKQLKKLGMLIVQDQIWNRVTINRAEKKSTRYY
                     MDEFHLLLKEEQTAAYSVEIWKRFRKWGGIPTAITQNIKDLLASREVENIFENSDFVL
                     MLNQAQGDRAILAKQLNISPQQMKYVTHTEAGEGLIFYGNVVLPFVDHFPKDTELYRI
                     MTTKPEEVSSL"
     gene            6037..7941
                     /locus_tag="JD016_11595"
     CDS             6037..7941
                     /locus_tag="JD016_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAU5000277.1"
                     /translation="MTRDGAVEINETQQTAERISSREADSDFSQPDSAAAERVMEHLD
                     AAHTRKASKKAVKKAQEATVLRTSTSRLQFTDEERETPKLQPYIKKSEKAADQLDAAK
                     AAIPKQKKLVKERTFEEATGKAKTRLHFEEQEKPISGGKAHSNPLSRPAQEAGIFVHN
                     KIHSVEKDNSGVEGAHKSEELAERGARYGTRKFKQGYRSHKLKPYREAAKAEQAAFKA
                     NVNFQYHKALQENPQLTSNPFSRFMQKQKIKCQYAKTVKKGGAATAKAAAGASQTAAK
                     KATAFAGRHPAGVIIAIAALLLFIMVSVGLSSCGAMFSGSMNSVLGTSYTSEDSDLVA
                     TEQSYAAMENELQQEIDNIESTHSGYDEYRYDLDTIGHNPHELASYLTALLQSYTPQS
                     AQAELKRLFGLQYTLTLTEEIEIRTTTDEEGNEEEYEYRILNVKLTNKPIASLAEELL
                     NPQQFEMFKVYLQTQGNKPLIFGGGSPDGSPSEDLSGVEFVNGTRPGNPELMELAKQQ
                     VGNVGGYPYWSWYGFNSRVEWCACFVSWCYNQAGKSEPRFAGCEWQGVPWFQSRGQWG
                     ARGYENIAPGDAIFFDWDLDGVADHVGLVLGRDGSRVYTVEGNSGDACKIKSYDLNYQ
                     CIKGYGLMNW"
     gene            7973..8245
                     /locus_tag="JD016_11600"
     CDS             7973..8245
                     /locus_tag="JD016_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAU5000278.1"
                     /translation="MANNKIDRINKEIAKTREKITEYQNKLKGLEAQKTEAENLEIVQ
                     LVRAMRMTPQELNAMLSGGGIPGMSAAPASDEAADYEEQEDNADEE"
     gene            8229..8960
                     /locus_tag="JD016_11605"
     CDS             8229..8960
                     /locus_tag="JD016_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAU5000279.1"
                     /translation="MQMKNKLKKTITALCAALILMGGFSVPAYAQGTAAEPPAEDATN
                     DSNVVVEEPETPPLTPKGNATLVDDFGGNKQLITVTTKGGNYFYILIDRAAEEEQTVH
                     FLNQVDEADLMALTETGEATKKPEVCNCTEKCEAGKVNTSCPVCATTLTGCTGKEPTP
                     TPTEQPEEPKEKGGNPGAVLALVLFVLLGGGAAFYYFKFIKPKQNVKGNTDLEDFDFE
                     DYDEDEPEADTGEVSKEEETEEEAL"
     gene            8957..9142
                     /locus_tag="JD016_11610"
     CDS             8957..9142
                     /locus_tag="JD016_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000280.1"
                     /translation="MTLFTDNPFEKMMIQKPDYGKREKSPPELSSPRCAGCPYKGQSP
                     CVGYCMRKLENKRQTER"
     gene            9235..9483
                     /locus_tag="JD016_11615"
     CDS             9235..9483
                     /locus_tag="JD016_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAU5000281.1"
                     /translation="MEDKKILLDNIDKIHTTEMGVDRIKRNLKIDTADVVEYCKNKVL
                     DKNCNIYKQGKNWYCEVENIKITINSYSYTIITAHIVK"
     gene            9598..11688
                     /gene="topB"
                     /locus_tag="JD016_11620"
     CDS             9598..11688
                     /gene="topB"
                     /locus_tag="JD016_11620"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAU5000282.1"
                     /translation="MILVIAEKPSVAQTIAAVLGAKEKKDGFLTGSGYIISWCVGHLV
                     GLSEAAAYGEQYKKWSYDSLPILPQEWKYTVSADKEKQFKTLKELMNRADVSEVVNAC
                     DAGREGELIFRFVYEMAGCKKPMRRLWISSMEDSAIKEGFSRLKNGEEYDALFASALC
                     RAKADWLIGINATRLFSVLYNHTLNVGRVQTPTLKMLVDRDAAITTFKKEKYYHVRLA
                     LSGAEAASERISDKAEADALKTTCEASRTVCTSLVKEKKTAAPPKLFDLTSLQREANR
                     LFGYTAKQTLDLAQALYEKRLLTYPRTDSAFLTDDMGDTAAGIIKLLCGKFSFMAGKD
                     FTPELAKVLNSKKVSDHHAIIPTMELSKTDLAALPESERNILTLAGARLLMATAAPHT
                     FEAVTAIFECAGQSFTARGKRVLSDGWKELDRRYRAALKNKPETDDADSDTEKTLPPF
                     TEGQTFENSAATVTEHDTTPPKPHNEASLLSAMERAGSEDTDPDAERKGLGTPATRAA
                     VIEKLVKGGFVERKGKQLLPTKDGINLVCVLPDTLTSPQLTAEWENNLTQIAKGKAEP
                     AAFMEGIEDMARELVKTYPFLSEADKGRFKEEKPELGKCPRCGSPVYEGKKNYYCSNK
                     ECIFTMWKNDRFFEERKVTFTPKIAAALLKSGKVNVKKLYSPKTGKTYDGTIVLADTG
                     GKYVNYRIELPKKK"
     gene            11930..>12174
                     /locus_tag="JD016_11625"
     CDS             11930..>12174
                     /locus_tag="JD016_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000283.1"
                     /translation="MASLRDTVKDYQEELRDGIAWVAFWKTGRSWNAEYFHLEMGDYL
                     YPEDRSRMEEIKQADPAAVVVNGYYSGYLGEDRNLDEL"
BASE COUNT         3526 a         2946 c         3080 g         2622 t
ORIGIN      
        1 ccttgcgccg ggagctgacc tgtccggcaa ggtgctgtcg cttggggagg gcttttctat
       61 ggctttttca aacccgcttc cgagccttgc ccctatggat ttactgatag gaattttagg
      121 cgcggtgctt atccggctga ttgtctatgt aaaaggcaag aacgcgaaga aataccggaa
      181 aggtatcgaa tacggctctg cccgttgggg aactgccgaa gatattaagc cctacaccga
      241 cccggtattt caaaataacg tgctgctgac acagacggaa cggcttacca tgaacagccg
      301 cccaaagcag ccgaagtatg caagaaataa aaatatcctt gttatcgggg gaagcggcag
      361 cggcaagacg agattttttg tgaagcccaa cttaatgcaa atgcacagca gctacgttgt
      421 aaccgacccg aaaggaacgg ttttagttga gtgtgggaag ctcttacagc ggggcggcta
      481 ccggataaaa gtattgaaca cgattaactt caaaaaaagt atgaaataca atccctttgc
      541 ctatctccgc agcgagaaag atattttgaa actggtaaac accttgattg ccaacaccaa
      601 aggggacggg gaaaaagccg gggaggattt ttgggtaaaa tcggaacggc tcttttactg
      661 cgcccttatc ggctacattt ggtatgaagc cccggaggaa gaaaagaact tcacgacgct
      721 gcttgaaatg ataaatgcgt cggaagcccg cgaggacgac ccggaatttc agtcccccgt
      781 tgacctcatg tttgaacggt tggaggaaaa agacccggaa cattttgccg tccggcagta
      841 taagaaattc ctgttatctg cgggaaaaac acgaagctct atcctcattt cctgcggtgc
      901 gcggcttgct ccttttgata ttaaggaact gcgcgacctt atggaaaccg atgaaatgga
      961 gcttgacacc ataggcgacc gtaagaccgc cctgtttgtt atcattagcg acaccgacga
     1021 tacctttaac tttgttgtga gtattcttta cacacagctt ttcaatctgc tttgcgacaa
     1081 ggcagatgat gaatacggcg gcaggctgcc cgtccatgtg cgctgtctgt tagacgagtt
     1141 tgcaaatatc gggcagatac cgaagtttga aaagctcata gccactatcc gaagccggga
     1201 aatctccgct tcaatcattt tgcaaagcca gtcgcagcta aaagcgatat acaaagacaa
     1261 cgctgatacc atagtcggca actgcgacac caccctgttc ttgggcggca aggagaaaac
     1321 caccctcaaa gaaatgtcgg aaatcttggg gaaagaaacc attgacagct tcaatacttc
     1381 cgagaaccgg gggcgcgagg tatcgcatgg gctgaattat cagaagttag gcaagcagct
     1441 tatgaccgaa gatgaaattg cagtcatgga cggcgggaaa tgtattttgc agctacgagg
     1501 ggtgcgcccg ttcttctctg ataagtacga cattacaaag caccccaact acaaatacct
     1561 gtccgactat gacaagaaaa atacctttga tatggaaaag cacctacggc gcagacctgc
     1621 ccttgtaaag tcggacgaac cctttgacta ttacgaaatc agcgaagcag atttgcagga
     1681 ggacaccgcc catgaataga cgtatccgaa agaaaaagga caagaaaatc acagaagcca
     1741 taaacgggct tatttccatt gccgagtggc aggaaagaca gtggcaggca gctatccagc
     1801 agtttgtaaa acggtgtgaa gccctgtatg agcagcaacg aaaggaggaa ttgacgcagt
     1861 aacaaagaaa aatacaactt acaggatacg cgcccctaca aaaattccta tcgctcaccc
     1921 caacaactga ataccgccgc gcggcagaca cataggcagc gcggggaaac cgccgaaatc
     1981 cggcgggctt atgaccgcgg cagtttgaaa actgaccgcc gttttttatg cccttttacg
     2041 gggcaagccg catttacggc agaaaggagc tttatggaat tttttaactc tgctatcgac
     2101 gtattacaga cccttgttat cgcactgggc gcaggcttag gcatttgggg cgtcatcaat
     2161 ctcttagagg gatatggaaa cgacaatccg ggtgcaaaat cacagggcat taagcagctt
     2221 atggcgggcg gcggtgttgc ccttatcggc actatccttg taccgcttct ttccggtctg
     2281 ttcggataag tttacggagg gtagcctatg gagagcatac tggaagcgat taacgaatgg
     2341 ataaaggaaa tccttatcgg agccattaac ggcaaccttt ccactatgtt cggggacgta
     2401 aacgaaaaag taggcaccat tgcagcggag gtaggcaaga ccccgcaggg gtggaatgca
     2461 aacattttta acatgataca aaatctgtcc gaaaatgtga ttgtacccat tgcggggctt
     2521 gtcattacct atgtcctctg ttatgagctt atcagcatgg taacggaaaa aaacaatatg
     2581 cacgacatag aaacattcat gttctttaaa tggtttttca aagcctttgt tgccgttttc
     2641 ttggtaacgc atacctttga tattactatg gcggtgtttg atatggcgca gcatattgtg
     2701 tccggcgcgg cgggggtaat cggcggcgat acgaatatcg acgtaaccga agcccttgcc
     2761 gccatgcagg agggactaaa agatatggaa atccccgaac tactgttact tgtcatggaa
     2821 acgagccttg taagcctttg tatgaaaatc atgtctgtac tgataaccgt tatcctctac
     2881 ggcagaatga ttgagatata cgcctactgt tccgtttccc ctatcccttt tgccaccatg
     2941 acaaaccggg aatggggaca gattgggaac aactacctaa aggggctgtt tgcactgggc
     3001 tttcaaggct ttctcattat gatatgcgtc ggcatttatg cggttttggt aggcgaaatg
     3061 gtcttagcgg acaatctgca cagcgcgatt ttttcccttg cagcctatac cgttatcctc
     3121 tgtttctccc tgttcaaatc cggcgcactg gcaaaatcca ttttcaatgc acattaacac
     3181 agcacaagcg ggtaagcccc gcagaaagga ggtcttttct tggcgtatgt acctgtaccc
     3241 aaagacttat ccaaagtcaa aacaaaagtc gctttcaatc tgacaaagcg gcaaatcctt
     3301 tgtttttcaa tggcacttct tatgggactg ccacttttct ttttgcttaa agacagcgca
     3361 gggacgagcc ttgcggcaat ggcaatgatt gtcgttatgc ttccctgctt cttggttgcc
     3421 atgtatgaaa agcatgggca gccccttgaa gtggtagtaa agaacatcat tcagacaaaa
     3481 tttacccgcc ccaaagtgcg accgtatcag acggaaaacc tgtatgcact cttggagaaa
     3541 cagcgggcat tagaaaagga ggtatcagcg attgtcaaaa ggacagacaa aaaagacgcg
     3601 ggaaaccgca ggaaacaggc gtaagctgac ccgcgcggaa aagaaacaga tagcggcggt
     3661 tatccggcag gcaaaggggg acggcaaagc ccacaccgca cagcagacca ttccctacct
     3721 tgccatgtac ccggacggta tctgcaaaat ggcacagcgc aggtattcaa agagcattgc
     3781 ctttgaggac atcaactatc agcttgcaca ggcagacgat aagaccgcca tttttgaaaa
     3841 ctggtgcgat ttcctcaatt actttgacgc aagcgtgaac gtgcagcttt ctttcatcaa
     3901 tcaaggctca cagcaggaac aggcagcgcg ggcaatccat atcccgccgc aaaacgacga
     3961 ttttaactct atccgcaccg agtattccga tatgctgaaa acgcaacttg ccaaagggaa
     4021 caacgggctt gtaaagcaca agtacatcac gttttccatt gaagccgaca atccggcggc
     4081 agcaagggcg cggctgtccc gcattgaaac ggacatactc aataacttca aggtgcttgg
     4141 cgtatcggcg caccccttat ccggctatga acgcttaaag gtgctgcatg gggtattcca
     4201 cccggcaggc gagccgttct cattctctta tgactggctg accccgacgg ggcttaccac
     4261 aaaggacttt atcgccccgt cctctttcaa gttcggggag ggacgctact ttgctatggg
     4321 gaaaaagacg ggggcggtat cgttcctaga aatccttgcc ccggagctaa acgaccgtat
     4381 cttagcggat atgttggatt tggaaacagg cgtaatcgtc aacttacata tccgcagtat
     4441 cgaccaggcg gaagcaatca agaccattaa gcgcaaaatc acagaccttg acaagatgaa
     4501 gattgaggaa cagaaaaaag cggttagaag tggttatgac atggatataa tcccgtccga
     4561 ccttgccacc tttgggaatg aagcaaagaa tttattgcag gatttacaga gccgcaacga
     4621 gcgaatgttt ctccttacgt tccttgtggt aaacatggca gacacgaagc ggaaactgga
     4681 aaatgatatt ttcgcggcgg cgggcattgc acagaaatac aactgcgcct taacccgtct
     4741 tgactatcag caggaagcgg ggctgatgtc ctctatccct ttgggggaga accttatccc
     4801 tattcagcga ggtttgacga cgagcagcac cgctattttt atcccgttca ttacacagga
     4861 gctatttcag accggggctg ccctgtatta cgggctgaac gctttaagca ataacatgat
     4921 actctgcgac cgcaagcagc ttaaaaatcc caacggttta atcttgggaa caccgggcag
     4981 cggaaaatcc tttgcggcaa agcgggaaat cacaaatgcc tttctcatta ccgacgacga
     5041 tatttttatc tgcgacccgg aagccgagta ttttgccctt gtaaagcggc ttggcgggca
     5101 agtgatacgc ttatccccga cagggaaagg catggacggt acaccccagt acgtgaaccc
     5161 tatggatatg aacctcaatt acagcgagga cgacagccct cttgcactga aaagtgattt
     5221 tatcctttcc ctctgcgagc ttgtcattgg cggcaaggag ggcttgcagc ccgttgataa
     5281 gaccgtcatt gaccgcgctg taaggaatgt gtaccaggac taccttgctg accctgtacc
     5341 ggagaaaatg cctatcttgg gcgaccttta caatgaactt ttgaagcagc cggagccgga
     5401 agctgcccgc attgcggcgg cattggagct gtatgtttcc ggcagcctta acgtatttaa
     5461 ccacagaacg aatgtggagc tttctaaccg ccttgtctgc tttgacatta agcagcttgg
     5521 aaagcagctc aaaaagttag gtatgctcat tgtgcaagac cagatatgga accgcgttac
     5581 tatcaaccgg gcagagaaaa agtccacccg ctactatatg gacgaatttc atctgctctt
     5641 gaaagaggaa cagaccgccg cttacagtgt ggagatttgg aaacgttttc gtaaatgggg
     5701 cggcataccg accgctatca cgcagaatat caaagacctt ttagcaagcc gcgaggtgga
     5761 aaatatcttt gaaaactctg attttgtcct catgctcaat caggcacagg gcgaccgggc
     5821 tatccttgca aagcagttaa acatcagccc acagcagatg aagtatgtaa cccacaccga
     5881 agcgggcgag ggacttatct tttacggaaa tgtggtactg ccctttgtag accatttccc
     5941 gaaagatacc gagctttacc gcattatgac gacgaagccg gaggaagtga gcagcctatg
     6001 aaacagttaa aaccacgcga taaaatcaca cagaaaatga cccgcgacgg tgcggtagaa
     6061 atcaatgaaa cacagcagac cgccgagcgt atcagcagcc gggaagcaga cagcgacttt
     6121 tcacagccgg acagtgccgc agccgagcgc gtcatggaac accttgacgc agcccatacc
     6181 cgaaaggcaa gcaaaaaggc agtcaagaaa gcgcaggaag cgactgtttt gcgtacttcc
     6241 acttcccgct tgcagtttac cgacgaggaa cgggaaacgc caaaactgca accctacatc
     6301 aaaaaatcgg aaaaagccgc cgaccaactg gacgcagcaa aggcagctat cccgaagcaa
     6361 aagaaactgg ttaaggagcg cacctttgag gaagccacag gcaaggcaaa gacccgtctg
     6421 cactttgagg aacaggaaaa gccgatatcc ggcggcaaag cccacagtaa cccgttatcc
     6481 cgccccgcgc aggaagcggg tatttttgtt cacaacaaaa tccacagtgt cgagaaagac
     6541 aattccggcg tggagggcgc gcataaatcg gaagaacttg ccgagcgcgg cgcaaggtac
     6601 ggaacaagga aattcaaaca gggctaccgc agccataagc taaaacctta ccgggaagcg
     6661 gcaaaggcag aacaggcagc gtttaaggca aacgtcaact tccagtacca caaagccttg
     6721 caggaaaacc cgcagcttac aagcaatcct ttttcccggt tcatgcagaa acaaaaaatc
     6781 aaatgccagt atgcaaagac cgtaaagaaa ggcggcgcag ccaccgccaa agctgccgcc
     6841 ggagcgtccc agacagcggc aaagaaagcg actgcctttg cggggcgaca cccggcgggc
     6901 gtgataatcg ctatcgccgc gctgcttctg ttcatcatgg tatccgtagg gctttcctcc
     6961 tgcggggcaa tgttttccgg cagcatgaac agcgtgttag ggacttccta cacgtcggag
     7021 gacagcgacc ttgtggcaac ggaacagagc tatgctgcaa tggaaaatga gctgcaacag
     7081 gaaattgaca atatcgaaag cacccacagc ggctatgatg aataccgcta tgaccttgac
     7141 actatcgggc ataaccccca cgaattagcg tcgtacttga ctgccctttt acagagttac
     7201 accccgcaga gcgcacaggc agagctaaag cggctgttcg gcttacagta caccttgacg
     7261 ctgacggagg aaatcgagat acgcactacc accgacgagg agggcaacga ggaagaatac
     7321 gaataccgta tcttaaatgt gaaactgaca aacaagccga ttgcttccct tgcggaagaa
     7381 ctgttgaacc cacagcagtt tgaaatgttc aaggtgtact tacagacaca gggcaacaaa
     7441 ccgttgattt tcggcggcgg ctctcccgac ggaagcccgt ccgaggattt aagcggcgtg
     7501 gagtttgtaa acggcacacg tcccggaaac ccggagctta tggaacttgc aaaacagcag
     7561 gtcggaaatg tgggcggcta cccctattgg tcttggtacg gctttaacag ccgcgtggaa
     7621 tggtgcgcct gctttgtgtc atggtgctac aatcaagccg gaaaaagcga accccgcttt
     7681 gcgggctgcg aatggcaggg cgtcccctgg tttcagtcaa ggggacaatg gggcgcacgc
     7741 ggttatgaga atatcgcacc gggcgacgct atctttttcg actgggattt agacggcgtt
     7801 gccgaccatg tggggcttgt gcttggcagg gacggcagcc gcgtctatac cgttgagggc
     7861 aattccggcg acgcctgcaa gattaaaagc tatgacctca actatcaatg tatcaaaggc
     7921 tacgggctga tgaactggta aaacagcccc aaaaagaaag gagaaatgac ctatggcaaa
     7981 caataaaatc gaccgtatca ataaggaaat cgcaaagacc cgcgagaaaa tcacagagta
     8041 tcagaacaaa ttaaaaggac tggaagcgca gaaaaccgaa gcggaaaacc ttgaaatcgt
     8101 gcagcttgta cgcgctatgc gtatgacccc gcaggaactg aacgctatgc tgtccggcgg
     8161 cggtattccc ggcatgagtg ccgcccctgc ttctgatgaa gcggcagact acgaagaaca
     8221 ggaggacaat gcagatgaag aataaactga aaaagaccat aactgccctt tgtgccgccc
     8281 ttatccttat gggcggtttt tctgtccctg cctatgcaca ggggacagcc gcagagccac
     8341 ccgcagagga cgcgaccaac gacagcaatg tagtggtgga agaaccggaa accccacctc
     8401 ttaccccaaa gggcaacgca acactggtgg acgatttcgg cggcaataag cagcttatca
     8461 ctgttaccac caaaggcggc aactacttct atatcctcat tgaccgggca gccgaggagg
     8521 aacagaccgt acatttcctt aatcaagtgg acgaagccga ccttatggca ctgaccgaaa
     8581 ccggggaagc cacgaaaaag ccggaagtct gcaactgtac ggagaaatgc gaagccggaa
     8641 aggtaaatac gtcctgtccg gtatgtgcaa ccactctgac aggctgcaca ggaaaagaac
     8701 ctaccccgac acccacagag cagccggaag aacccaaaga aaaaggcggc aatcccggcg
     8761 cagtgcttgc cttagtcctc tttgtgctgc ttggtggcgg cgcagcgttc tactacttta
     8821 agtttataaa accgaagcag aacgtgaaag gaaacaccga ccttgaagat tttgactttg
     8881 aggactacga cgaggacgag ccggaagcag ataccgggga agtttccaaa gaggaagaaa
     8941 cggaggaaga agccctatga ccctgtttac cgacaatccc tttgaaaaga tgatgataca
     9001 gaaaccggac tatggcaaac gtgaaaaatc cccgccggag ctttcttccc cacgttgcgc
     9061 gggctgccct tacaaggggc agtccccttg tgtggggtac tgtatgagaa aactggaaaa
     9121 taaacgacag accgaacgat aaaaagatgt acttgcgata tggaatatga tataattgtt
     9181 ccaaccatag gaaatgacag agacaaacaa acttgaagtt gaggaaaaga aagaatggaa
     9241 gataaaaaga tattgcttga taatattgat aaaattcata caaccgaaat gggagttgat
     9301 agaattaaaa gaaacctaaa aatagataca gcagatgttg ttgagtattg caaaaataag
     9361 gtattagata aaaattgcaa tatatacaaa cagggtaaaa attggtattg cgaggttgaa
     9421 aatatcaaaa ttactatcaa ttcatatagc tatacaatca ttacagcaca tattgttaag
     9481 taattttcag tttgtcaaat tatttttata tttcatatcg cacaatttta tacaatcact
     9541 taggagcaat cgaaaaatcg gttgctcttt tcttttaccc gaaaggagga tttttatttg
     9601 attttagtta tcgcagaaaa gcccagtgtg gcacagacaa tcgctgccgt acttggggca
     9661 aaggaaaaga aagacggatt tctcacagga agcggctata tcatttcttg gtgcgtggga
     9721 catttggtag ggctttccga agccgccgcc tacggggaac agtataaaaa gtggagctat
     9781 gacagcttac cgattttgcc gcaggaatgg aagtacaccg tttcggctga caaggaaaag
     9841 caattcaaaa ccctaaagga gcttatgaac cgggcagatg tttccgaagt cgtcaatgcc
     9901 tgcgacgcag ggcgcgaggg agaactcatt ttccgttttg tctatgaaat ggcgggctgc
     9961 aagaaaccta tgcgccgcct ttggatttct tctatggagg acagcgcaat caaagagggc
    10021 ttttcccgtc tgaagaacgg cgaggaatac gacgcgctct ttgcttccgc attatgcagg
    10081 gcaaaggctg actggttaat tggtatcaat gccacccgcc ttttctctgt cctttacaat
    10141 cataccttga acgtggggcg cgtacagacc ccgaccttaa aaatgcttgt tgaccgggac
    10201 gcagcgatta ccactttcaa gaaagaaaaa tactaccatg tgcgccttgc cttatccggc
    10261 gcggaagctg caagtgaaag aatttcagat aaggcagaag ccgacgcatt aaaaacgact
    10321 tgcgaagcat cgcggacagt ctgtacttcc cttgtgaaag agaaaaagac cgcagccccg
    10381 ccgaagctct ttgaccttac ttctttacag agggaagcaa accgcctgtt cggttatacc
    10441 gcaaagcaga cccttgacct tgcacaagcc ctgtatgaaa agcgtttgct tacttatccg
    10501 agaaccgaca gcgcatttct aaccgacgac atgggcgaca cagcggcggg cattatcaag
    10561 ctgctttgtg ggaaattttc ttttatggcg ggaaaagact tcacgccgga gcttgcaaag
    10621 gtgctgaaca gtaaaaaggt atcagaccac catgccatta tcccgactat ggagctttca
    10681 aagaccgacc ttgctgcgct gccggaaagc gaaaggaata tccttaccct tgccggggcg
    10741 cgtctgctca tggcgaccgc cgcaccgcat acctttgaag cggttacggc aatctttgaa
    10801 tgtgccggac agtcctttac cgcaagggga aaaagggtac tgtccgacgg gtggaaagaa
    10861 cttgaccgcc gctatcgggc agccttaaag aacaagcccg aaacggacga cgcagacagc
    10921 gacacagaaa agaccctgcc gccatttacc gagggacaga cctttgaaaa ttcggcggca
    10981 acggtaacgg agcatgacac aacaccgcca aaacctcaca acgaagcgtc gctgctctct
    11041 gctatggagc gcgccggaag cgaggacacc gacccggacg cagaacgcaa agggcttgga
    11101 actcccgcca cccgcgccgc cgtcattgaa aaactggtaa agggcggttt tgtggagcga
    11161 aaaggcaagc agctacttcc cacaaaggac ggtatcaacc ttgtgtgcgt cctgccggac
    11221 accttgacaa gcccgcagct taccgcagaa tgggaaaaca atctgacgca gattgcaaaa
    11281 ggcaaggcag aacccgccgc atttatggag ggtatcgagg atatggcgcg ggaattggta
    11341 aagacctatc cgtttctttc cgaagctgac aaggggcgtt tcaaagagga aaagccggag
    11401 cttgggaaat gtccccgctg cggttctcct gtctatgagg gaaagaaaaa ctactattgc
    11461 agtaataagg aatgtatctt taccatgtgg aaaaatgacc gtttctttga agaacgaaaa
    11521 gtaaccttta ccccaaagat tgccgctgca ctcttaaaat ccggcaaggt aaatgtgaaa
    11581 aagctctatt ccccaaagac gggcaaaacc tacgacggaa ctatcgtatt ggctgatacg
    11641 ggcgggaaat atgtcaacta ccgcattgaa ctaccgaaga aaaaataact gaatagcaag
    11701 cataggaaaa gagtaccctt ttccgtaaaa tccctgcttt ctctaccgag aacggcgggg
    11761 atttttgctt gttgggaagt gtcccaaacc ctctgttttg tggagggttt taccctctga
    11821 aatcttgaaa aaatctataa aatctttggc aaaaacgcgg gcgcggggta ctgaatgatg
    11881 aaaccggaac atgcagcgtc cgcgctgccc tttggaaagg aggttacaca tggcaagttt
    11941 acgggacacc gtaaaggact atcaagaaga attacgggac ggtatcgctt gggtggcgtt
    12001 ttggaaaacg ggacgttctt ggaacgccga atatttccac cttgaaatgg gcgactacct
    12061 ttacccggag gacagaagcc gcatggaaga aatcaagcag gctgaccctg ccgccgttgt
    12121 ggtaaacggc tattattccg gctatcttgg cgaggacagg aaccttgacg aact
//
LOCUS       DACXMZ010000110        12053 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.111, whole genome shotgun sequence.
ACCESSION   DACXMZ010000110 DACXMZ010000000
VERSION     DACXMZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12053
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.111"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1127)
                     /locus_tag="JD016_11630"
     CDS             complement(<1..1127)
                     /locus_tag="JD016_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAU5000284.1"
                     /translation="MTIPKLEPNDIKEEFNKYTSEQINKVVYNYLFKKNMSSKRIDEE
                     ILDLQFKQGKEYQSMEILNNLGINKKHKGIFIGCTIDKAIEVLKEDEIHFKQIINILL
                     ESKNINEYPIIVDSWELLDEKIALKTIDKSTLKHNGTGVPIEIRHFFEINNLGKNDKK
                     NVNFRYDDNYYSAVIEMDKLENPRSRIIWKSKFRDLLRNIFPKYYEKFLEGKIEENAN
                     LPKIRFAKLDSNNFDIEFIFTNEIEDDVLEEISQDNTLDVDTINVELETEIRKEGKVK
                     YVYGKQYERNPKNRIEAIKYHGTKCVVCGFDFEKIYGNRGKGYIEIHHIRPLSSVGKE
                     SNINPKTDLVPICSNCHRMIHRKKDSVLSIDKLKKIVKDSK"
     gene            complement(1401..1676)
                     /locus_tag="JD016_11635"
     CDS             complement(1401..1676)
                     /locus_tag="JD016_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000285.1"
                     /translation="MYDEIIEIYFDYWINEHMYRDILEKDKYYIESWKEIMAINESLE
                     KSLLEENKIYVLMELNDLFNSLIEFERELSYKLGFKAGAKFIIELLK"
     gene            complement(1924..3258)
                     /locus_tag="JD016_11640"
     CDS             complement(1924..3258)
                     /locus_tag="JD016_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAU5000286.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVQELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFMSY
                     VSIVIEKYKNRNIIDKFNKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEINEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDDEIVKYIQALSIINQENNLY
                     INDSVNTVSGIKREIKNIKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            complement(3271..4014)
                     /locus_tag="JD016_11645"
     CDS             complement(3271..4014)
                     /locus_tag="JD016_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000287.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITILLMASHKENYV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWEIYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIREYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYHSKEIVINKGTGTYNPDDSQLKW"
     gene            complement(4036..4350)
                     /locus_tag="JD016_11650"
     CDS             complement(4036..4350)
                     /locus_tag="JD016_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000288.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKVIENAKRNK
                     AELITCKEASRLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            complement(4354..4554)
                     /locus_tag="JD016_11655"
     CDS             complement(4354..4554)
                     /locus_tag="JD016_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5000289.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKEE"
     gene            4701..5465
                     /locus_tag="JD016_11660"
     CDS             4701..5465
                     /locus_tag="JD016_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5000290.1"
                     /translation="MQIGKNLKNIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYFLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDI
                     KEIIKNDVLTKQSIDELLDIGLSEENIETIYDYFSDPTDTGSNIRLKKLISQKENITI
                     KNNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            5522..5719
                     /locus_tag="JD016_11665"
     CDS             5522..5719
                     /locus_tag="JD016_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000291.1"
                     /translation="MNKSYILKYTYKCPTNLFHGDKKYTLIYIKRIMSKLVYDMDNLE
                     NNSFTCLEVQSLFDNIFKVKL"
     gene            5716..6207
                     /locus_tag="JD016_11670"
     CDS             5716..6207
                     /locus_tag="JD016_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5000292.1"
                     /translation="MMNNTIGEKIKQYRKSVNMSRDELANKISCSVHAIAKYEQNQRM
                     PSLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFNKEFDLVIHILENYDYDI
                     KTDYKTKFEITDNTDTIKPINLNFSDFKSFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            6296..7048
                     /locus_tag="JD016_11675"
     CDS             6296..7048
                     /locus_tag="JD016_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000293.1"
                     /translation="MNKSYIPKYTYKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVSDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            7038..8204
                     /locus_tag="JD016_11680"
     CDS             7038..8204
                     /locus_tag="JD016_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAU5000294.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHMQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNTDILKAGELYRNDNYVFCNELGIPIDDKRPLRNLKSILNSLDIELIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(8320..8556)
                     /locus_tag="JD016_11685"
     CDS             complement(8320..8556)
                     /locus_tag="JD016_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000295.1"
                     /translation="MIMEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKL
                     ISEGVADMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(8556..9032)
                     /locus_tag="JD016_11690"
                     /pseudo
     CDS             complement(8556..9032)
                     /locus_tag="JD016_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103284.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
     gene            complement(9032..9247)
                     /locus_tag="JD016_11695"
     CDS             complement(9032..9247)
                     /locus_tag="JD016_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HAU5000296.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            complement(9563..9912)
                     /gene="ssrA"
                     /locus_tag="JD016_11700"
     tmRNA           complement(9563..9912)
                     /gene="ssrA"
                     /locus_tag="JD016_11700"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(10070..10561)
                     /gene="smpB"
                     /locus_tag="JD016_11705"
     CDS             complement(10070..10561)
                     /gene="smpB"
                     /locus_tag="JD016_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAU5000297.1"
                     /translation="MEQISSKEGEIYMAGKKILATNKRARHEYFIEETYECGIELKGT
                     EVKSIRQGKVNLSDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIR
                     KLIGATTIKGYSLIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMT
                     GKY"
     gene            11124..11951
                     /locus_tag="JD016_11710"
     CDS             11124..11951
                     /locus_tag="JD016_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HAU5000298.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT         4244 a         1824 c         1366 g         4619 t
ORIGIN      
        1 tatttactat cttttacaat tttcttcaac ttatctatac ttaaaacact atcttttttc
       61 ctgtgaatca ttctatgaca atttgagcat attggcacta aatctgtttt gggatttata
      121 ttactttctt ttccaacaga acttaatggt ctaatatgat gaatttctat gtatccctta
      181 cctctgtttc catatatttt ttcgaaatca aacccacata caacacactt tgtaccatga
      241 tattttattg cctctattct attcttaggg ttacgctcat attgtttccc ataaacatat
      301 ttaactttcc cttcttttct gatttcagtt tccaattcta cattaatagt atcaacatct
      361 aaagtattat cttgacttat ttcctctaat acatcatctt ctatttcatt agtaaaaata
      421 aattctatat caaaattatt actatcaagt tttgcaaatc ttattttagg taaatttgca
      481 ttttcttcta ttttaccttc taaaaatttc tcataatact taggaaatat atttcttagc
      541 aaatctctaa acttagattt ccaaattatt ctacttcttg gattctctaa tttatccatc
      601 tctataactg cactataata attatcatca tatctaaaat tcacattttt cttgtcgttc
      661 ttccctaaat tatttatttc aaagaaatgt cttatttcta ttggaacccc tgtcccatta
      721 tgttttaatg tagatttatc aattgttttt aaagctattt tctcatctaa taactcccaa
      781 gagtcaacta ttataggata ctcatttata tttttacttt ctaaaagtat attaataatt
      841 tgcttgaaat gtatctcatc ttctttaagc acttcaattg ctttatctat tgtgcatcct
      901 ataaaaatac ctttatgttt tttatttata cctaaattat ttaatatctc cattgactga
      961 tactctttac cttgtttaaa ttgtaaatct agtatctctt catctattct tttactactc
     1021 atatttttct taaataaata attgtaaact actttattta tttgctctga tgtatattta
     1081 ttaaactctt cttttatatc attaggctct agcttaggta ttgtcatatt cccctccttg
     1141 attgattatt ttagatttta ataaaattat tgtatcatat atttattata aaaacatatt
     1201 gtgttattct attaaataca caaatactgc aacataatta attgcataaa aaattaaaga
     1261 gggcatcctt ataccctctt tatgaaatat atttatttta aacaatataa ttaaaacaca
     1321 cttaattcaa agtattatag tacaacttat taataatctc agtttgctat ttaattgttc
     1381 tactgaaata cacaaattaa ttacttcaac agctcgatta taaattttgc tcctgcttta
     1441 aagcctaatt tatagctaag ttctctttca aattctatca atgaattaaa taaatcattt
     1501 agttccatca atacatatat tttattttct tctaataaac ttttttctaa tgattcatta
     1561 atcgccataa tttctttcca actttcaata taatatttgt ctttttctaa aatatctcta
     1621 tacatatgtt catttatcca ataatcaaaa tatatttcta taatttcatc atacatttta
     1681 gtacctcctt gactttcatc aggcacttaa tatatactta agttgctagg ttagtgtaca
     1741 ttaagtacct aatttataaa ggtagttgtt atatttatat ggcaactacc tttttctaat
     1801 aagcttttaa atctaaactc aatatatttt aaatctctat tgtatctaat taaaatttat
     1861 atttatcctt caatacttct tgaaacataa tttatctatt acttaataaa tcatgcttct
     1921 tttttatatc tcttgtatct tcttacattc ttcaaaagaa aacaaatttc ctatatacca
     1981 atatggtcta aatcctgact caccatccct atttttagca acaataatct cacttaaatt
     2041 agcaccaaaa cctttttctc ttcgtctcag ctctcttata tcctcaattc ccattgtctt
     2101 acaataatta agagcttctt tttctgtagg tctatgaata tatataaccg aattagaatc
     2161 catatatatc tgctttgagc ttctcattac tctatcccct ttgggtcttt catctttcaa
     2221 ttcatcattt aattgagcaa gctgaataac tggtatatta aaatctaaag taatcttttt
     2281 tatttgcctt gataagtcag cgaatttagc ttctcctact tctttacctt cataatccaa
     2341 aatttgcaaa taatcaatta ttactaaact aggcttaata ttctttattt ctctctttat
     2401 tcctgatact gtgtttacag agtcatttat ataaagatta ttttcttgat ttattatgct
     2461 taaagcttgt atatatttga ctatttcatc atcgtttaga tttctatttt tcatctttac
     2521 aactgatatt ccagttagtg atgctatatt tctaaacaaa atctgtatat cgctcatttc
     2581 tccacttata aaaagtactt tcttaccttg aaatattaca tttctaagta tttgtaacgc
     2641 caatgctgtt ttacctcttc cacttttagc tgctattgta gttagttcac ccccatataa
     2701 acctcctata gtatcatcta agaactttat tccagtttta ataccttcat ttatttcatc
     2761 atttatatat tcaaatgctc tctctgatat ggctgttata cttgaatcct tttttattaa
     2821 tttctgatta gctttattta tatcattttc taactgatat ataagagtat tatcatctat
     2881 ttctccaaga ttgaacttat tgaatttatc aattatattt ctatttttgt atttttcaat
     2941 aactatactt acataactca tgaaatttga tactgttgga actatggttg acaaactagt
     3001 gatatagctt atacctccaa tattattcaa atgacctttt ctctttaatt cttctgtaag
     3061 agttattata tctattactt catttctctt atttatttct tttatagcat taaatatgta
     3121 tttatgttcc tcatgataaa acatgtcttc atttaacatt tgctctaact cttgtactat
     3181 tgttgaatct aataaaatac ttcctaatat tgattgctct gcttctgtat ttttaattaa
     3241 gttgtttgaa ttatttataa tataacctcc ttaccacttt aattgtgagt catctggatt
     3301 atatgttcct gtccctttat ttatgacaat ttccttgcta tgataattgc cctctagtat
     3361 cttggtaaaa ttacttgaat tcattatcca atcaaaattt gctttccatc ctgtattatt
     3421 atttccattc aaaaaacttg ttttgctaac tatatttata acctctaata cagtttcttt
     3481 atcatattct cttattcttg aatttattct cctctttctt gtgtctgtta gtttcttgac
     3541 ctttgaaagt tcaatacaag ttgagttaaa tttgcttata atttcactat atataatatt
     3601 attttcattc tttatattct tgattgttgt tgattgattg ttaatcgtct gttgattagg
     3661 ttgttgattt tctatgttct cttggtaaat ctcccagttt tcaatagtta tgactatata
     3721 tttgtttgtt gtttcgtatg ttagaaaacc agcattttta aaaaccaaca aacaacttct
     3781 aaccatttga taactaactc ctttaccaca tctttcagct atcttgctat aggaagaaat
     3841 aaattgtcca gactttattt ttatcctttt gttatctata aaaacatagt tttctttatg
     3901 actagccata agtaatatag ttattaatat tacttttctt tctggtttaa tgtctttcca
     3961 aattgattta tccaacaact ccctataaag ctttatccac cctttattac tcaattacaa
     4021 acacctactt taagactata acttgatacc atttgcattt tcaataaacc aatcatccaa
     4081 tttatttttt attatgtata ctcgattatt tatccatata caagggaatc ctttattttt
     4141 agaatgacat aactgcctaa attttgattc tcctatagga taaagtctcg atgcttcctt
     4201 acatgttata agttcagctt tatttctttt cgcattttca ataactttat atccttgctt
     4261 tttttcttgt gcttcttctt cattctttac aataatgttt aaagtagctt gtaatgtctt
     4321 taaaacttct aaattattat tctctttcat actttattcc tccttactaa aaaataattc
     4381 ttctactgtt gtatctaaag ctctagatag tttaatcatc aaatcattgc ttggattatt
     4441 agccttacct gtttccaagt ttgacaaata atatcttgat ataccaactc tatctgctag
     4501 ttcttgttgc tttagacctt ttttaatcct aaatattttt aaattgattc ccatactttt
     4561 tcctcctttt ttctagttta cattcgtaaa ctttacattt taattataat gtcgacatat
     4621 gtatactgtc aataaaaata tattattttt ctgttctcat ttgtgtactt ttgggctata
     4681 ctttatacga ggtgataata ttgcaaattg gaaaaaactt aaaaaatata agaaaacaaa
     4741 aagaactaac acagattcaa cttgctgaaa ttagtggcat ctcaagaaat gctctaataa
     4801 attatgaaaa tgataaaaga atccctagta tagatacctt atcaaaactc gctaaagctt
     4861 tgaaaattga aaagactatc ttttattttg atattgaaaa tgaagtaatg actaatgaag
     4921 atgttttata ctttttacag tctttattcc cagaagaata ctattctttt gatgaatttt
     4981 taaatgctat ttcaaatctt atggaagatg atgatattag aattacctca agtttattgc
     5041 taggtgcttg gtctgataaa gaccaatatt ataaacttgg tgaaaaatta gaactaaatg
     5101 aaaatcaagt ttataattgg atactttcag attgtatatc taatactgaa ttagattttt
     5161 cattacatcc tgatgacata aaagaaatta ttaaaaatga tgttcttaca aaacaatcaa
     5221 ttgatgaatt attagatatt ggtctatcag aagaaaatat agaaacaatc tatgattatt
     5281 ttagtgaccc aacagatact gggagtaata ttcgactaaa aaaattaata tctcaaaaag
     5341 aaaatattac tattaaaaat aatgattact ttaaattatt acaagaaaat aacgaattaa
     5401 aggaaaaaaa tgaaaagctt gaaaatacac ttaaagaaat aaaaaaacta ctaactaaaa
     5461 tataaaataa tatcaatact atctaaagca atgataaaaa actatgaatg gagttacaat
     5521 tatgaataaa tcatatattc taaaatacac atataaatgc cctactaatt tatttcatgg
     5581 agataaaaaa tatactctaa tatacattaa aagaattatg agtaaattag tatatgatat
     5641 ggataatcta gaaaataact cctttacatg tttagaagta caatcattgt ttgataatat
     5701 atttaaggtg aaattatgat gaataataca atcggagaaa aaattaaaca atatagaaaa
     5761 tcggtaaata tgtcaaggga tgagctagcc aacaagatta gttgttcagt tcatgctata
     5821 gctaaatatg agcaaaatca aagaatgcct agcctagata tgataagaaa aatagctact
     5881 gctttatcaa ttgctataaa taaactcatt gttgaagaaa atgatttaga tactttaaca
     5941 aataataata ttaatttaaa agtatttaat aaagagtttg atttagtaat tcacatatta
     6001 gaaaattatg attatgatat aaaaacagac tataaaacaa agtttgaaat tacagataat
     6061 acagatacaa taaaaccaat taacctaaac ttctctgatt tcaaaagctt ctctaaaaat
     6121 ctaaattggg tgataaatgg tcttgttgaa aattttatac aagaaaactc caatggtctt
     6181 attgaagatg aagatgaaga atattagtat tgcacatttt caaaatatat atttacttat
     6241 taattttgat atagtcataa ttagataaga attaaataag gaaggagcta caatcatgaa
     6301 taaatcatat ataccaaaat acacatataa attccctact aatctacctc atggagataa
     6361 aaaatatact ttaacataca ttagaagaat gataagtgaa tttgtatatg atatgggtaa
     6421 cctagaaaat aaccccttca cattcccaga agtacaaaca ttacttgatg gtataacagt
     6481 aggtggacat aaattaagtg accaaaatca aatactaaat ataaaatcat catgggatta
     6541 tgtaataaaa ttaagtaaca aagaaaactt atctttaaat aaagatacaa tttgctctat
     6601 acataaaata gttgcaaaag atgaagctct aatcgttgga gatttcagaa atggtaacat
     6661 aggtatagct ggtactactc aatatgaatg catagaagcc actctattaa atgatttatt
     6721 tgtaagtgac ataaacatca taaataaaat aactaacccc ttagaaaagg ctataataat
     6781 aaatttatgg ttgtcctatt gtcaattctt ctatgatggc aacaaaagaa ctgctagact
     6841 tacctcaaac ctaatactta taagtaatga tataggagtt ctatcaatac cagctaaaca
     6901 taaacaagaa tacaacacat taatgctaaa cttctatgaa acactagaag ctgatgaagt
     6961 tataaaattc ttattagaga aatgtattac attctttgat ggatataact ataaatcata
     7021 taaggagctg tttaaaaatg gcaactaaaa gagctaatgg agaaggttct atcggaaaat
     7081 ataaagatgg ttggcgttcc cgtataatga ttggctataa tgaagatggt aaaccaataa
     7141 gaaaagaatt ttatggtaag actcaaaagg aagttaaaga taaactagat gcttataaaa
     7201 aacaatatta tttaagctct gatatttcag atgataaaat aactttagag cattggtttt
     7261 acaattggtt gtttgaatat agaataaaag atttaaaacc taagtcattt gaaagatatg
     7321 aaagcatcta tcgtaattat attaaagata ctgatttagg caatgttaaa ttaaaggact
     7381 taagagtatc tcacatgcaa aaatattaca atagattgct tgattccaat aaaagtatac
     7441 ctactataag gcaaataaac actaaactga aaacctgctt atcagaagct gaaaaacaag
     7501 gtcttataca acgtaactat tgtactatgg taaatatacc agttgagaaa aaggaaaata
     7561 agcttgaaat attgactctt gaacaacaaa agagttttat aaaagctata gatggacata
     7621 aattagaggt attattttta cttgctcttg gtactggact tagattagga gaattattag
     7681 ggcttaaatg gtttgatatt gattttaaaa agtctaattt aacagttaaa agaactcttc
     7741 aaagaacata ttttatagat aaaactggaa atagagagct taaagtatta gagcaagaac
     7801 caaagacatc aaattcttac agaactgtac ctattccaaa agacgtttta aataaattga
     7861 aagagcataa agtaaatcaa aatacagata tattaaaggc tggagaatta tatagaaatg
     7921 ataattatgt attttgcaat gaattaggta tccctataga tgataaaaga cctttaagga
     7981 atctaaagtc tatattaaac agtttagata tagagcttat aaagtttcat ggtcttagaa
     8041 aaacttatgc tactagactt tttgagaatg atgtccctcc taaaactgta caagttttaa
     8101 tgggccatta tgatatatca ataactttaa atatatatac tcaagttatg gaagataaga
     8161 aggttgaagc tgtagaaaag ttggataaaa tattttcatt gtaaaaatca aatacatata
     8221 aatattgtga tttcatatag ttaattacaa aatcaaaata aggatacaaa ttaattttat
     8281 attatcttgt atccttattt cgtatctagt tttaattatt tatcttggat agcctttatg
     8341 gtacattaat ccattttcta acattaattc ttgagccaag tatatatgac ccttacagtg
     8401 catatctgcc acaccttcac ttataagttt atatgtttca gaagtatata aataatccat
     8461 actttcttcc aagctcttac ctgtttgctt agaaaattct aatatgattt ttttatatag
     8521 tatttgtaat aatgttttat ttgcttccat aatcactata cctcctcttc acatctaaac
     8581 tttaaatatt tatctattac attttgattg gttatacata tttgttcatt tggtttatag
     8641 tattttaatc tttttaaagc ttcatctttt tcaataagat tatcttgata cagttctatt
     8701 gtattgtaaa tcttattatc agcaatgcca ctattataat gtcatatttc atatatatta
     8761 ctcatatttc tacattctat tataaaatca agccattcta aattataact attgaagatt
     8821 tttaccttgc agttttcttt taatttatca atttctaaag aataactatt taaatatgct
     8881 tttttacctc tttttttaaa acgctctgtc cagttaattg cttgtgtctt tatactggtt
     8941 acatagaatc cttttccaaa atctaaagaa tctcttgaaa aggtaatact aggcgtcttt
     9001 accacacaat atgaaccatg atatacaatc attatatcac acccttttct ttaagtactt
     9061 gcattaaatc ttccactatg taacctttac tttgagaatg tagtgcttca tagcaaggta
     9121 ttatatagtt atctaaaata tcagtatcat gtttaagaag tttataaact ccttctgaat
     9181 ctatattcaa tttttctgct aaagcttcaa tgcaaaaaat agaaaattct agttttcttt
     9241 catccattac aaacctcaac tcctatcaag cttatataat atatattttt actaattaac
     9301 aacttaaact gtcttacatt tataaaaata ttatatcata aagtagatat taaaattcta
     9361 tgttacctat tatcataata aacaaataaa ataataaaca aataaaaact ttttatacag
     9421 cctaaatgca ccccaatata cccccaaaaa aacctcattt tctgtggttg gggtacggtt
     9481 ggtgaatttc ttctccaaaa taaaaaatac tagtcaacaa agactagtat ttccaagtat
     9541 ttcatcattc ttttttcaaa attggtggag acgaggggag tcgaacccct gtccgcaaat
     9601 cttttccttg ctgcatctac atgcttagcc aacttcacta atatctcacc tcacatagcg
     9661 ccaagtggca ggctctatgc ttagccagtc ccaattaatc tacttcaagt cgagacaccc
     9721 ctaaagtagt tccctgcata atgacgtcca gtcttagtcg gcaggaggac taagagggac
     9781 gagcagcaac taggctgcta tcgcgaaatt atcgtctgcg tttattttaa agttccccag
     9841 tttttacgtg acccagagta acacgacatg cagcctcaag ttccaaaccc acgtcgaaac
     9901 ctttacgccc ccttgagtag tttatagtcc agtagacttt tcgcaaactt tatcaccatt
     9961 tttattaaat ttttaatgta tatataatat aatactttta agaataaaaa tcaatgttat
    10021 gttctagata ataaagaatt ttattctaaa aaatacagtt ttatctagtt taatacttac
    10081 ctgtcatttc tctttctata cgtctttgtg catctttctt agccatatct tgacgtttat
    10141 catatagttt tttacctttt gctaaggcaa tctctacttt aactttaccg ttttttagat
    10201 acaaactaag tggtataagt gaatacccct ttatagtagt tgctccaata agttttctta
    10261 tttcatgctt atgaagtaat agttttctaa ctcttaaaga atcaacattg aatatatttc
    10321 cttgctcata tggacttata tgaacttgtt ttaaaaatac ttcgctatta tctacagatg
    10381 caaatccatc acttaggtta actttgcctt gtcttattga ttttacttct gttcctttta
    10441 attcaattcc acattcataa gtttcttcta taaaatattc atgtcttgct cttttgtttg
    10501 ttgctaatat ttttttacca gccatataaa tctcaccttc tttgctagaa atttgttcca
    10561 taaatgttag aaatttacta aatatttaat gttgccaaat tatttgacta acttgtatga
    10621 taacatattt aggcatttta gtcaatattt cactgatata ttaagttagt taggctatat
    10681 ataattaagt aatataaatg tatgaaaata gctaaaaaac tacttcgaat atacttaata
    10741 ctaaactaac tctaaccagt aatactaatt tataaatttc aaaacaaaaa tattacatat
    10801 tataaatctt ctaacttcta acttctaact tctaacttct aacttctaac ttctaacttc
    10861 taacttctaa cttctaactt ctaactaatt ttaacatgcc cataaaatat aatacccaca
    10921 aatgataaat aatactcaaa atatctattg aaataactat acatccttaa ataacttaac
    10981 ttaaatctaa acatttcaaa aacttcttaa aaatatgtaa aaatttgatg ataaagtagc
    11041 gattttttca aaaaaaatgt aatatagagt tatatcttat aatattttag agataaattt
    11101 tatcatagtg gggagaaatt tgtatggatt taagcaaaaa agtaaaaatt ttagaaaatg
    11161 catcagatgg caaaagcgaa tttgagatat tagaatttga aaaacttgag ggagcttcta
    11221 gcccattaat ggcaatggga ttatattttg ctagagaagc tggtttgaaa gctagacaaa
    11281 ttaagataaa attaaataat tcatcaataa aaacagaagc tggtgcatta tactattata
    11341 aaggaaatat atcttctgaa actaagattg gtggtgttgg aggtcttttt aaaaaaggaa
    11401 tatcaggagc acttactaat gaatcaatga tgaaacctgt atacaaaggt actggagaag
    11461 tttatttgga accttctttt agacattatt tatttatgag tcttgataat gattctataa
    11521 ttgttgataa aggattattc tattgttgct ctgatacaat agaagtaaaa gcatttacac
    11581 aaaagaactt ctcatctgct ttacttggtg gtgaaggctt cttccaaata gaattaagtg
    11641 gtactggtgt aatcgttctg gaatgtgttg tacctcaaag tgaaattgta gagtatgaat
    11701 tgaaaaatgg tgaagaatta aaggtagatg gtaactttgc aatagcaaga acatctggtg
    11761 ttaatttttc tgttacaaaa tcagataaat ctttaattgg ttctgcttta aatggtgaag
    11821 gtttcttaaa tacatttact ggtaatggta cagtttggct agctccaact caagtaatgt
    11881 atgaaaaact aagttttgga gcaatgccta ctaatgataa tatgaataat aaagtttcta
    11941 agcaaaaata gttgttttat aaatatttat taaatcatta ttgattttat aaagtcacta
    12001 taaattttat tagattatta ttataaaatc agagtgtgga aatatttatg tgt
//
LOCUS       DACXMZ010000111        11973 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.112, whole genome shotgun sequence.
ACCESSION   DACXMZ010000111 DACXMZ010000000
VERSION     DACXMZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11973
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.112"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            65..397
                     /locus_tag="JD016_11715"
     CDS             65..397
                     /locus_tag="JD016_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAU5000299.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            544..744
                     /locus_tag="JD016_11720"
     CDS             544..744
                     /locus_tag="JD016_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000300.1"
                     /translation="MFIKKMSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQK
                     KKYDRLNKDTIERAIVEAINNN"
     gene            771..1856
                     /locus_tag="JD016_11725"
     CDS             771..1856
                     /locus_tag="JD016_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAU5000301.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            1849..2307
                     /locus_tag="JD016_11730"
     CDS             1849..2307
                     /locus_tag="JD016_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000302.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            2342..3433
                     /locus_tag="JD016_11735"
     CDS             2342..3433
                     /locus_tag="JD016_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAU5000303.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            3438..3662
                     /locus_tag="JD016_11740"
     CDS             3438..3662
                     /locus_tag="JD016_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000304.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            3672..4235
                     /locus_tag="JD016_11745"
     CDS             3672..4235
                     /locus_tag="JD016_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000305.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            4196..4825
                     /locus_tag="JD016_11750"
     CDS             4196..4825
                     /locus_tag="JD016_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000306.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            4865..5002
                     /locus_tag="JD016_11755"
     CDS             4865..5002
                     /locus_tag="JD016_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAU5000307.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            5002..5754
                     /locus_tag="JD016_11760"
     CDS             5002..5754
                     /locus_tag="JD016_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000308.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISIDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            5767..6252
                     /locus_tag="JD016_11765"
     CDS             5767..6252
                     /locus_tag="JD016_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000309.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            6259..6408
                     /locus_tag="JD016_11770"
     CDS             6259..6408
                     /locus_tag="JD016_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000310.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(6544..7365)
                     /locus_tag="JD016_11775"
     CDS             complement(6544..7365)
                     /locus_tag="JD016_11775"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAU5000311.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            7858..9552
                     /locus_tag="JD016_11780"
     CDS             7858..9552
                     /locus_tag="JD016_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAU5000312.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      9884..9994
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10098..10469
                     /locus_tag="JD016_11785"
     CDS             10098..10469
                     /locus_tag="JD016_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HAU5000313.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            10565..11512
                     /gene="trxB"
                     /locus_tag="JD016_11790"
     CDS             10565..11512
                     /gene="trxB"
                     /locus_tag="JD016_11790"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAU5000314.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            11616..11933
                     /locus_tag="JD016_11795"
     CDS             11616..11933
                     /locus_tag="JD016_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HAU5000315.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT         4736 a         1246 c         2145 g         3846 t
ORIGIN      
        1 aaacttataa tataattgta tctgtaaaat tttgtaaaat ataaaaagta tagggggaat
       61 aaaaatgaaa gtagcttata tttttagtag tactaataca cataaaattc tagacaaaat
      121 gataatacca caacttgaac aaggaactca tggagtagac gttctaggga tgtttttctt
      181 tatggataat accttatttt tatctaaagg taatcctgta ggcgagagac taagtaaatt
      241 acatgaaaaa actgatatga taataatggc ttgtgaccaa tgtgctatag aaagaggtat
      301 agagaataat ttggttgatg gagctacaat tggatgtttc ccaaacctat atgcttgttt
      361 aggcagtgca ggtgtggacc aagttataac gttataaatt agtataggat acaatccata
      421 gaaaaattta ttttctgtgg attattttaa gtaaaagata aattgtttaa agtaggggat
      481 taacaaatgt ctaaaatttt agttgaattt ataaacactt gaccatcttg tgatgaacat
      541 ggagtgttta taaaaaaaat gtcagaaaag tatgctgata aattggaaat aaaattatat
      601 caagcaggga aagatttttc gtatataaaa aaatatggga ttataacaaa aggaacttta
      661 ataataaatc agaaaaagaa atatgataga ttaaataaag atacaattga aagggcaata
      721 gtagaagcta taaacaataa ttaaaagttt aaaaattagg agtatcaaaa atgaatatat
      781 tagaaattgt tttaaatgga gttaaagata ctatagtagc ttctatagtt acactgaatg
      841 gagcatcagg atggcttatg ataagtttta tattggcagg tgtacttcac aatgttttaa
      901 ctccaagaag gtttcaaaaa caacttggaa ataagaaatt ttcttcttta attaaaagta
      961 ctatctcagg aatgttttta ccaatatgta gttgtggagt aataccactt ggaataagta
     1021 tgtattattc aggagcatat ttaggaccag tacttgcttt tatgacatca actccaataa
     1081 taaatccaat agctgtagtt ctttgtctag gtcttttagg tcctaaagtt acaatcattt
     1141 atctaatagc tggtttttta gtaccatttt tagtaggatt tttaggaaat tgttttggaa
     1201 aagaagaatt atcacttcat aatgattcag aggaagaagt aatagaatta gaagaagaaa
     1261 aaagaagttt tatgggaaat atgattgatg gtcttaaatg gtcttttggt gaattagccc
     1321 ttactattag taaatatgta gtaatgggta tggtttttgc aggatttata acaactattt
     1381 ttccaaatag tataattcaa aaatatttag gaaatccagg tttgctatct cttggaagta
     1441 tagcaatact tgcatgtgtt atgtatgtat gtgctgtagg acatataccg tttatagcag
     1501 cccttgttgc aagtggagca tctcctggta tagcgataac atttctaata gctggagcag
     1561 ctacaaatct accagaactt ataagtatgt ttaaacttat tggaaaaaga actgttatta
     1621 tatattctgt aactttgaca ctgtcttcaa taataattgg atatataaca aatttacttt
     1681 tagtgaataa taactcagta aatttagatg caacaaattc ttcaatacag ttggcaaata
     1741 aactaatgtt aaacatacct gatagttttg agtatgtatg tactatttta attattttat
     1801 ttgcatttaa atcaataata ccaaagatga aagaggtcat aggaaatcat gcttaaaaag
     1861 atagttataa tatcctgtat agtagttgtg ttaatcattt tatcaaaaat agttgatgat
     1921 aacatcaaag aagatgcttc tattccaaat gtgaataaag aaacattaga atattttaga
     1981 aaaaattata aagaagacat aataacttgt gctgaagaag acttaaataa tgatggaaaa
     2041 aaagatttag tagttatata taaaaaaagc aataattcaa atgaaatggt agtcgtagtt
     2101 tctgataaaa atagccacta tataacaaag ccaataccag cacctataga gaatcaaact
     2161 ataaccttta aaaatattga tgataaagca ccaatagaag taattgtatc agggtctaaa
     2221 aatggtaatg taggatatgc tatttacaga gtagagggta aaaaatttgt tgatttattt
     2281 ggcgaagata tggacaaatg ttgctaaaaa taaaaataat acataaaggt gggaaattta
     2341 tatgaaatta tctaagaaga ttcaagcatt aatcttattt ggtgtaatgg gtacatctat
     2401 attaacagga tgtagtagta aaccaaaaga aaaagaagaa gcaaaaactg gaaaagatga
     2461 ttatacaata acagtaggtt attataattg tgaccatatg actgcgggac cagtagcaga
     2521 atcggcagga atatataaag atttaggtct taatgtaaaa actgttggaa atggtaaagt
     2581 tcctgaagct atggcagcag gaaaaatgga tgcaggttac ataggaacta aggggttagt
     2641 tggagcaata cctaagggtt caccaataac aatagcagct aacaaccata ctggtggttc
     2701 tgaatattta atagtttcaa aagatataaa agaacctaaa gatttaatag gtaaaaaaat
     2761 tgcaacagat atgagtgatt tcttatggac aagtgattat ggtccagaaa caggtctacc
     2821 aactgaccca tcaaaatatg aagttgtaaa tatggattca gacaaagata aatatttagc
     2881 tcttaaaact gggaaaattc aagcttttac atcttgtgac ccttggggtt ctgtagctga
     2941 aaatgacggt gcaggaaaaa taatagcatc tactcaatat aaagaaaaag ctaatggcaa
     3001 agaatataac tgttgttcat tctcattaaa caaaaacttt ataaaagaac atccagattt
     3061 agcaaaaaaa ttggttctag cacatacaaa agcaatagaa tatatatata caaatccagc
     3121 tgaagcagct aaaatatttg caaaatatta taatgtagaa gaagaagttg cacttagaac
     3181 tatctataag aagactgttg gagaaggtag aactcttact tggaaagtta caggtgaaga
     3241 atataaaaat aatttacaaa tgtataaaga tttaaaggct ttagatgata tacctaaata
     3301 tgaagatacg attgatacaa gcttattgga ctcttgtgga gctgatgatt ttgacaaatt
     3361 tataaaagaa aaagttaacc cagagtttcc tgaaggtatg agttatgaag actggaaagc
     3421 aaaggtatta taaataaatg gagaatatga aaaatataat aatacttttt atcattattt
     3481 tgggaagtat atttataata ttttttaatt ttgaagataa agaagaaata aaaggaaaat
     3541 taagaatagg ggtatctgat gatacctctg gttttgtaat aaattatatg gtaaatagag
     3601 actactttga aaatataaaa gtaagtgata taatggatat ttttacaatt aatgattgct
     3661 gagcaagtac aatgcagtgg gctctgagtt cagaggaaat agatatagca atcatatgta
     3721 aagatgctgc aaaacaatat gtaaatgtaa atagtggatt tgaaattgtt ggtactgtag
     3781 ttcaaaattc tgatatattt ctattaagtg ataataatcc tgaaaagata ggagtcattc
     3841 aaaatagaga ttaccagtca gaattggtta aaaaatacta caagaatgtg gaagtagctc
     3901 ccttattagg gactgcacta gcatatggat tagaaagtaa tttagtagat ggtgttgtaa
     3961 tagattgcat aaagtcaatt gggttagagg gcaaaagaaa atctacaact aaattaggag
     4021 attatgatac ctatgtatta gttgtaagta aagagtttaa aaaaagtaaa gcatatagtg
     4081 attttataac actttacaat aaaagtgttg atgctttaaa gaataaagct acatttaaga
     4141 aagcattaaa agagtacagg aatataagaa atatagatat ggaggaaata agcaattgga
     4201 aattaaaatt tctaaagata aaagcgatag attaactaag aaaaaaactg atggtagtat
     4261 ggatatttta tataaaataa tactattagt tattatattt ttagtctggc attttgcagc
     4321 aaaagatata gggagttctt tgttacttcc aatgccagta gatgtaataa aaggtttctt
     4381 tttctgtgtg acagatgcag agacagtcac taatcttttt ataacattgc agagagtact
     4441 taaaggtttt atgtatgcat tattgtttgg gcttccaata ggatttatta tgggattttc
     4501 taaaactttt gaaagggttt tatctccagt agtagattct gtaaggcaag ttccaataat
     4561 ggcatgggtt cctcttacta tagtatggtt tggtatagga gatggtccaa caatattttt
     4621 aatagctttt tcaggggttt tccctataat tttaaatact attcaaggag taagagcaat
     4681 atcaaaggat tattataatg cagctagaag tatgggagca tcgccaattg tgatatttac
     4741 aaatgtaatt gttcccgcat ctcttccaga tatactaact ggttcaagga ttgctataag
     4801 tacaggatgg atgtctgtta tctgagccga gtttatagct acgagtgccg gtctcggtta
     4861 ctcaatggta caagctcaaa cattaatgca tacagatgta ttaattgggc ttatgatatt
     4921 tgcagcttta ataggattta taatagatag ggtattaaag tttataaata aaatcctttg
     4981 tagatggagg tttgcagatt aatgaagtta agtgtaagag atataaataa gacatttgta
     5041 aataatagag tacatacaaa ggttttagaa gatataagta tagatataga tgatggacaa
     5101 tttgtctgtc ttttaggacc atcaggttgt ggaaaaacaa ctttacttac tataataggt
     5161 gggtttcaaa aatcagaacg tggagatgtt tttataaatg ataaaagagt taaaaaaccg
     5221 ggcatagata gagcttttat atttcaaaat tatgctttat ttccttggaa aactataaga
     5281 ggtaatgttc tatttcctat gaaacagcag aaaataccaa aagaaaaaag agaagaaatg
     5341 ctagaagaac ttttggttat gtcagacttg aaaggaaaag aaaaactgtt tccgcatcaa
     5401 ttatctggag gaatgaaaca aagagtggcc atgataagag ctttagcatg taatccagag
     5461 gtattactaa tggatgaacc attaggagct gtagattttc aaatgagaca aaatttacag
     5521 gaagagctag aacgaatatg gataaagaag aaaataactg cattaatggt aactcatgat
     5581 gttgatgaag ctgtatatat gagtgataga gttatagtaa tgtcgagaga taaaggaaga
     5641 attattgatg atataaatat agatattcca agacctagaa ttagaggaag ccaaaaatat
     5701 gaagagtaca aaaacaaatt aactgatact ttatcaaagt gttatgaagt ttaaagagga
     5761 gtagacatga gtcaaataga aaaatacaat agtttggata atacacaaca gaaaataaga
     5821 atctctatta tggatatgat aatagataaa ggttctagtg ttacacttca agaagtgact
     5881 gaatatgtaa gtaaaaagtt gaatatagaa aaagaatata tagaaaggac actacaatat
     5941 tttatttata aaaatataat ggttgttgat gggaatagta taaattttat atatcctgta
     6001 tcagcattgc caacaaatca taaagtaact ttgagagata atagaagttt cagtgcaatg
     6061 tgtgctatag atgctatagg aacctcttgc acattcaacc aagatataaa aataaactct
     6121 atttgtagtg ctacaggtaa ggaaatagaa gtaattataa aaaatgaaaa gattgagtat
     6181 gtgaataatc caaacttaag agtgcttcat ataaatcttg ataagcatct aaattgggct
     6241 gctagttgct gaaatataat gaatttcttc tggacgaaga gtgattttga tgcttggaca
     6301 aacgaagcgg gattatctga tgatgaagat atatattgtt tagatattaa tgaggcaata
     6361 gtagaatcat ataaaatatt taaactcaaa caaaaggtac tatcatagat atacatatat
     6421 gaaaaactaa aaatgacctt tagataaaaa tgataggtaa tatccatact atataaatta
     6481 gtatagagta gggatgtgaa aagtatttaa atctaaaggt atatttttag tttttttaga
     6541 tgattagata gccttattaa gtatatattt atttataacc tttgcaactc cattttcatt
     6601 attagtatca gtaacataat ctgcaatttc ctttatacta ttacgtgcat tccccatagc
     6661 cacaccaaga cctgcatact ttatcatatg ttggtcattt tcttcatcgc caacagcaat
     6721 agcattttct ataggtatat ttagattcgt acataatgtt ttaagagcag ttcctttatt
     6781 agtagtttta cctaaaaatt ctagataaaa aggagcagac cttactatag tatatttatt
     6841 gaataaattt ttaggtagtt gctgtatgat ttcttccaat ctttctggtt catcaactat
     6901 cataatttta cagattttaa tagattcatc aattttatct ttttgtatat atttgacttc
     6961 tatattattt aatttcgctt catgaagtgt atatttgcca ggcgtttcat ttggtgtaat
     7021 taaatttgtc attgtattta tgtgtatttt agtcttatat tttttacaaa cgttattata
     7081 tataaaatca aaatcatcta aagtcatttc tacatcatgt atgattttct cacttttaac
     7141 ttcttgaata attgctccat taaagcatat aacataatca ccttcatctg ttaaatttag
     7201 ttgatttaga agttctttta caccaggaag aggtctgcct gttgttataa ctacttttgc
     7261 cccagccctt tttgcctcct gaatggactc aaagacttca ggagtaatcc ttttttgtgt
     7321 atttaatatt gttccatcaa tgtctagtgc gattaatttg tacatatcaa cctccaataa
     7381 attatatact caaatttcct gtgaaaacgt tgacacagct agtgttgaac aaagtttcag
     7441 tgggaaatat ggtttgtttt gtatactatt atatcaagag ataagtaatt tagaaagttt
     7501 aataatatta attttttgat aaaaatatta cttatgtgag atgagaatgt aaaaactttg
     7561 taatcaataa ttctaaaatt cttaattaca aataatggta tcgtttgcat tgttaatata
     7621 aataaatgca tattatatag ttagaagttt gaataaatag actgatggta atttgttata
     7681 ttagttagtt ttgtgttata aaatattgta attccattaa tcatatgtta ttattcaaca
     7741 tgattataat ataaagaata tttgatatat ataataattt ataaaagagt attacatagt
     7801 acatgtaaat ctatatttat aaatacaata aagtaaagtt gtaaaggagt gctttaaatg
     7861 aagttttcag agtttaagta tgaaagaccc aattataata gtatgaaaaa agagtttttg
     7921 agttgtgtag aagatataaa taattctcgt aattataaag agcaacaaaa aaatatacat
     7981 aagattaatt tattaagaaa taaaatagaa acgttgtcaa atatagcttc gataagatat
     8041 agtacagaca catttaataa attctacaaa gaagaaaaaa attattggga tgaatatatg
     8101 cctttgtatg aagaattaaa ttcatatttt tataatgcca ttgtaaattc aaaatttaaa
     8161 tatgatttga taaaagagtt tggagagcaa tttttcacaa tagtagagta ttctttaaaa
     8221 agtttttcca aagaaataat aagtgaattg caggaagaaa ataaattgtg ttcagagtat
     8281 actagacttt tagcttcagc tgagataatg tttgatggga aaataagaaa tttatctggt
     8341 atggggaaat tcatgtattc taagaataga aaaacaagag aattagcaaa caaagcatat
     8401 tataatttct ttgaagaaaa tgaaactaaa tttgatgaca tatttgataa attggttaaa
     8461 cttagagata agatgtctaa aaaattaggt tttgaagatt ttgtagagct tgggtatgtt
     8521 agaatgatga gaagtgacta cagagaatat atgataaaaa atgttagaaa acaagtatta
     8581 aaatatgttg ttccaatggc aaatgagctt tatgaaaaac aagctaaaag aattggtcta
     8641 gaatatttaa gttacataga tgaaggcgtg gaatttttaa caggaaatgc aagtttaaag
     8701 ggagattcaa gatacataat tcaaaatggt aaaaggatgt attctgaact gtcaaaagaa
     8761 acaaatgaat tttttgattt tatgttagaa aatgaactta tggatttaga aacgaagaaa
     8821 ggaaaaggag ctggagggta ttgtacttat ataccagatt ataaatcacc attcattttt
     8881 tctaatttta atcaaactgc tgatgatata gatgtattaa cacatgaggc aggacatgca
     8941 tttcagttgt atatgtctag atggattgat atgccagaaa ttaattttcc aacgcttgat
     9001 agttgtgaaa tccattcaat gagtatggaa tttataacat ggccgtggat ggatttattt
     9061 tttaaagaag atacagataa gtacaaattc actcacttat catcttcaat taaatttata
     9121 ccttatggag taattgttga tgaatttcaa cactatattt acaaaaatcc taatgtagat
     9181 aagtcaaaac gaaaagaaat ctggagattt ttagagaaaa aatacttacc tcatagaaaa
     9241 tatggtgata attctttttt agagagaggt tgttggtggt ttaaacaagg acatatattt
     9301 aaaaatccgt tttattatat agattatgta ttagctcaaa tttgtgcgtt acaattttgg
     9361 aaaaaaatga ttcaagatag agatgcagga tggaaagatt atataaatat ctgtaaagtt
     9421 ggaggaacaa aatcattttt agatattgtg agtatgggaa atttgtattc tccatttgat
     9481 gatggctgta ttgagtctat tataggtgat gttaaatctt ggtttgatga aataaatgac
     9541 agcaaattat aaaaaattat gagaatttta ttgtataaaa gtgaaaaatt aaaaaatata
     9601 taaaaagtga ttatttgttg tgaaaatagt cttgttttta actaaattga taaaataaac
     9661 taaaaaagat ttgtacttaa aaaaaaaaga aaatacaaga aatatgcaat atcaacattt
     9721 ggaaaaaaaa gtaaaaatgt atacaaaata catataaaaa aactattgca aattttatag
     9781 aatgtttata tacttaagtc aacagagata gaaaaaagct atttatagaa aacaaaggat
     9841 aaattgctta atttaaaatt ttctgaaaaa aataataatc cggatgaaga gtatgggaga
     9901 ctgctttata aaaagcgacc gaagggacaa gtttaatctt acttacccaa aagattagat
     9961 gaaattctca ggtaaaggtg actgtacttg gacggggctc tgaaaatgat tttaacaggg
    10021 aagacaaatt gggagatttg tttttcctgt tttttattta tcaagtaata aataaaagtt
    10081 tgaaagagag taaaaaaatg ataataatta cgaataatcc aaaagtaaaa gaagaagttc
    10141 aaggcagaga agttttgttt aaagacacaa cttatattgg aatcttagaa gcaagtagag
    10201 atttgataca tgaaggttat gaacttttat ctcatccact ctatgggagt gtaaagccaa
    10261 atgaaacacc ttacaggact gttatactaa agaaaggaaa tcgtttggat ataaactctt
    10321 taaccttaat tgaagaagca atcatcactg caagtaaatt tcagaataat aaaaagactc
    10381 caaaatggac agaaagtgtt caagatgatt ttagagtaat tgattatgat atattttata
    10441 atacaataca aagaatgcag tatgaataaa aaaatttgtt aaaaagttat taaaacagcc
    10501 taaatattaa atctaattta atattaaata aaatgagaaa tttaattggg gaggataagt
    10561 caaaatggaa aatgtgtatg acttagtaat aattggttca ggaccagcag ggcttgcagc
    10621 aggtctttat ggagcaagag caaagttaaa aactctaatc ctagaaaaag acaagactgg
    10681 tggacaaatt gtaataacac atgaaattgc aaattatcca gggtcagttc caaatgcaac
    10741 aggaccaagt ttaatagcta gaatggttga acagtgtaaa gagtttggag cagaaatgct
    10801 aagagacaac attgttgata ctgagctaga tggagatata aaagttttaa aaggggaaaa
    10861 ggcagaatat agagcaaaag cagtaataat aggaacagga gcaacaccaa gaaagattgg
    10921 ttgtcctgga gaaaaagaac ttactggtaa aggggtttca tattgtgcaa cttgtgatgc
    10981 agatttcttt gaagattttg aagtatttgt tgtaggtgga ggagacagtg cattagaaga
    11041 agcaatgtat ttaactaaat ttgctagaaa agtaacaatt gtacatagaa gacaaggatt
    11101 tagatgtgct aagagtgttg aagaaaaagc aaaagctaat ccaaagatag agtttttatt
    11161 agatacagta attgaagaaa tcaaaggtga tggaatatta gaatcagttg tatttaaaaa
    11221 taaagttact ggagaaactc atgaatactt tgcagatgaa gaagatggaa caatgggagt
    11281 atttgtgttc gttggattag atgctcaaac tgatttattc aaaggtaaag ttgatatgga
    11341 tgaaaaaggt tacataataa ctgatgaaga tatgagaact aacataccag gcgtatttgc
    11401 agcaggagat tgtagatcta agactctaag acaggttgta actgcaacta atgatggagc
    11461 aattgcatct atagtagctg agaaatacat tgatgaaaaa tttgggaact agaatctgta
    11521 aattaagaaa tagtgtctgt aaatttaatg aataatattt aaaattaaaa ttaatattta
    11581 aaattaaaat aaaaatttca agggggaata agaaaatgtt agatttagat aaggctacat
    11641 ttgaagaaga agttttaaat gcagaaggtt ttgtatttgt tgatttttgg agtgaaggtt
    11701 gtgagccttg taaagcttta atgccagatg ttcataaatt agctgagact tatggggata
    11761 aaataaaatt ctgtaagatg gatacaacaa aagctagaag attagctata aaacaaaaag
    11821 tattaggtct tccaactatg gctatataca aagatggaga aaaagtagac gaagttacta
    11881 aggatgatgc tacagttcct aatatagaaa acatgattaa aaaatatctt taatatgttg
    11941 ataaattatt aacattaaaa gaaaaaatct att
//
LOCUS       DACXMZ010000112        11837 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.113, whole genome shotgun sequence.
ACCESSION   DACXMZ010000112 DACXMZ010000000
VERSION     DACXMZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.113"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(597..2738)
                     /locus_tag="JD016_11800"
     CDS             complement(597..2738)
                     /locus_tag="JD016_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAU5000316.1"
                     /translation="MLLSIVMMVKNEENILEKTLKSLSVLRNSIESELIIVDTGSTDN
                     TIRIAKQYTEKVYFHNWNDDFSSMRNISISYAKGEWLLILDADEVLIDYSNIIKFFES
                     GLNKKFNSASVRLKNLYSFDKKLYGYCSVLRFFKNLDFKYCGKVHEQPMYKNPIFNNI
                     ADFEHYGYLFEDEEIRINKVKRNEKLLLEELKEDEKSPYTNYQIGKNLIILGKYQEAL
                     DYLEKSYKLYSKLEIVPGYLITNLAKVYLYLGKAKKCEKLCLKYINKDRNNIDIYYHI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEMSTQANSLLSDTDAVGFRDEAIITLIKIYY
                     KLEKYDLVISEYDNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYREIPNSKVERDSF
                     YKSMEDFINNIKDDEKELIYKSLCDIEGNYGLLNKIRINKHVSLDTCRKILNEEKDIV
                     YASVIKIAFEEKVDLLDIFYNMEYVWIEKYLRYILALERSFNVSIYKYIISCPNTSEI
                     EKIRVYKVISKILLESVNLSDKKYKELFYIYIMYSYKYIKYIYSNLSDCELIKFVCND
                     IDKFTLEFKHVLDGKRKINNPNEKLEYLHNMRILLNEYPFYNRIIKIFISELEESITE
                     SEEFKLLKDNFIIRIENMIENGKINDAKLLIEDYSKTFADDVKILNIKAILFMCEDKF
                     EEADFALKKALSLDLENEDTIYNIEYLKNLK"
     gene            complement(2832..3704)
                     /locus_tag="JD016_11805"
     CDS             complement(2832..3704)
                     /locus_tag="JD016_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HAU5000317.1"
                     /translation="MRVNTNVSALIANNQMGRNVNGQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGSLEETGNILQRMRTLSLQSANEIN
                     NTEEREKIADELTQLKDEIERISSSTEFNGKKLLDGTSSTIRLQVGASYGTNVSGTSN
                     NNNEIKIQLVNTASIMASAGITTASIGSMKAGGTTGTDAAKTMVSSLDAALKSLNSSR
                     AKLGAQQNRLESTQNNLNNTLENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            complement(3805..4140)
                     /locus_tag="JD016_11810"
     CDS             complement(3805..4140)
                     /locus_tag="JD016_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HAU5000318.1"
                     /translation="MDKLEEKINLYKDISLKIINFIEKMEYKNISFQLDERQNIINSI
                     SEVDKSEFIQLYDSMELFEIDAKIRDALQEQLSEVKKELHEYKLTKQVNTMYYSSNRE
                     KVNIFNKKV"
     gene            complement(4143..5666)
                     /gene="fliD"
                     /locus_tag="JD016_11815"
     CDS             complement(4143..5666)
                     /gene="fliD"
                     /locus_tag="JD016_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAU5000319.1"
                     /translation="MSSISPVRVTGLSGNFDMEGIIEASMIRDKEKVNKAKQDQQIVK
                     WKQEIYRDIIKESKNLYDKYLNGDSPNSITNKKAYSATRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKVTIKLNSSDPIVQQFPPNASGASSLNIGGVNIPISEQDTTSTI
                     VSKINSLCADNDIRASYSEMTGELIISRKQTGSSSDIDLKVIGNDSLAGQIASDNGIT
                     FTTDASGTKSAVVYGKNLEADVTDDQGRVTHISKEQNSFKIDNIDYNVNSKGSAKLVS
                     VTDTEEATKNMKAFVDDYNALMDKVYGLVTTKKSKDYPPLTDEQKDDMTTEEIEKWEK
                     KAKEGILRNDDELRAFVEDIQSMFFGDADTIIALRKLGISEHENYNKKGQISFNADTF
                     SKALIDDSDKVYKALAGYSSNYDDKGMFEKLKKIVFEYSGSSASKLTKKAGMENSSSA
                     SQNVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNKYSSQMNYFSQAQGN"
     gene            complement(5694..6047)
                     /gene="fliS"
                     /locus_tag="JD016_11820"
     CDS             complement(5694..6047)
                     /gene="fliS"
                     /locus_tag="JD016_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAU5000320.1"
                     /translation="MMELDLNELNGLSKEELLLMLVDGTVKYTNISKVALLNKDYLKA
                     HNELVRVQNIFTELMVTLDQSVGQWAKDMYKVYEFIKHELVKADINKDIKIIDDILPI
                     VEQIRDTWHEVYNKL"
     gene            complement(6044..6439)
                     /gene="fliS"
                     /locus_tag="JD016_11825"
     CDS             complement(6044..6439)
                     /gene="fliS"
                     /locus_tag="JD016_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAU5000321.1"
                     /translation="MYGENPYNSYKQNAIFMASKEQLLLMLVDGAVKYTKIARGAIID
                     KNTRKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDVQII
                     DNVLPVIEEIKDTWHEADRKSREERSRYK"
     gene            complement(6461..6673)
                     /gene="csrA"
                     /locus_tag="JD016_11830"
     CDS             complement(6461..6673)
                     /gene="csrA"
                     /locus_tag="JD016_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAU5000322.1"
                     /translation="MLVISRKKDEAVLIGDNIEVKVVGVDGNNIKLAISAPNNISILR
                     KEIYEKVKNENIKATNKNIKILKSLK"
     gene            complement(6667..7059)
                     /locus_tag="JD016_11835"
     CDS             complement(6667..7059)
                     /locus_tag="JD016_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HAU5000323.1"
                     /translation="MMKITLKKGILGFENLKEYELLDIENEDILKEFNSTEEDCIGFI
                     VVSPFEIIKEYEIVLNQETIEKLEVKSPNDIMLLNIITVGQTLEESTVNMKAPIVINV
                     RNNCGMQIILQDEEYSIWHPLLRGDGGC"
     gene            complement(7075..8007)
                     /gene="flgL"
                     /locus_tag="JD016_11840"
     CDS             complement(7075..8007)
                     /gene="flgL"
                     /locus_tag="JD016_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HAU5000324.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQINTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYIENCKDTADWLETTDTSLDQLGNLLADIKKGLVSSGNGSY
                     SDDEIKTISNSTNEKMKEIANALNATHEGKYIFSGSNTGTPPVECVENADGSVSLKFN
                     TSLNLNKLNDSLSVDVAQGISVDYNVKLSSLGFDPTKTDPFEKLNNISKKLANPSDAN
                     IKELTTTCLGDMENLIDNTVNVRSIYGTKANTVDAMKEKNDEGLIQLKDVLSQNDEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            complement(8026..9336)
                     /gene="flgK"
                     /locus_tag="JD016_11845"
     CDS             complement(8026..9336)
                     /gene="flgK"
                     /locus_tag="JD016_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HAU5000325.1"
                     /translation="MAGLFSILNTANSGMNAHQKSIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIGRGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKGDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDLENSDVKNMLSDGKLELNELKNADGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNSSAGITVRDFFITSNVAGEDIIKVNPAIKSNSNELKLTTEEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNVDNSR
                     MSVSGVSLDEEMTELMKVQRSYQASAKVMSTAVQLLDVVINGII"
     gene            complement(9354..9770)
                     /locus_tag="JD016_11850"
     CDS             complement(9354..9770)
                     /locus_tag="JD016_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HAU5000326.1"
                     /translation="MITDLKVIIYEEKKIISNMMELLKEQFDYIIEKDLENLNRLNPK
                     LEDISRELASIEIKRRQLLGDDVSISEVVENSNDEYLKEIYIDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPVDKQTTTYNSYGNIRK"
     gene            complement(9775..10050)
                     /gene="flgM"
                     /locus_tag="JD016_11855"
     CDS             complement(9775..10050)
                     /gene="flgM"
                     /locus_tag="JD016_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HAU5000327.1"
                     /translation="MNIKSVSSNIVVNNYLKNKSDVKKVQQVQKVDKTDKIELSKFAK
                     EMSSVSNMSDEAARNKKIEEIKSALENGTYKIKPEEIAKKMIEEMRG"
     gene            complement(10201..10515)
                     /locus_tag="JD016_11860"
     CDS             complement(10201..10515)
                     /locus_tag="JD016_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HAU5000328.1"
                     /translation="MNSKKEDIYELNFGQLIEEKTGIIEENQNVFNAKVNVAVSIGNI
                     TETIKNILDLKEGSIIKLNKNVDEKLDIYANDRLFAYGESIITNETLSVRLSKIEDSD
                     VN"
     gene            complement(10539..10967)
                     /locus_tag="JD016_11865"
     CDS             complement(10539..10967)
                     /locus_tag="JD016_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HAU5000329.1"
                     /translation="MGINIDKLNISPSRSIEVKKTKENNKFSDSLNFVKQSSSLEDIK
                     RNLDGVKKAGERLVLTQNYGDISRYKNAVKEYLKSVVDNMYSLDKSSSFWEHQYYKNV
                     KIIDNKLEDMTSKLLSEEKENIDIVSTVDMIQGLLIDMYR"
     gene            complement(10976..11644)
                     /locus_tag="JD016_11870"
     CDS             complement(10976..11644)
                     /locus_tag="JD016_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAU5000330.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCGYNNSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSEKLFNELDSVANNTVGKFIEVDTKDKSVKSRIENAVEQA
                     SKKYNVDANLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTNPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMRSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
BASE COUNT         3577 a         2042 c         1162 g         5056 t
ORIGIN      
        1 tttattataa aattgtatat agctcaatca aaaagctaaa aactaaatct tatcaaaata
       61 aagaaatctt gcgttcttaa ctaacaaaaa tactctataa atggttttta agtattaaaa
      121 tgacttaata aaaattcaat tattacaaat actgctgttt tatgtcgaat taattcaatt
      181 atttaaaaac actaaaatca ttgaaatcac taaaaaattg tcttaatgtg caaaaccttc
      241 gtttttagga tattgcccga tttaattata tacatattat cgtctaattt taaaaaaaat
      301 taagagaaaa tatgtataat tttcatttat attgtttttt gttgtttatt ttttgaacat
      361 gcttatctat tttattatta taagtaaata tgataaaatt agtttccctt gaattaaatc
      421 ttctatatac taaaatcatt gagtatattc tattttattt gatgccaata tcgatatata
      481 ttgctgtacc agtaaatttt acatcagtat ttttatatgt atatattcaa tcttatatta
      541 tatttttcat taactgttat tgatattatt attttctaaa taacctagtt ttaaatttat
      601 tttaaatttt ttaagtactc tatattatat atagtatcct cattttctaa gtctagtgat
      661 aatgcctttt ttaatgcaaa atcagcttct tcaaatttat cttcacacat aaacaaaatt
      721 gcctttatat ttaaaatctt tacatcatct gcaaatgttt ttgagtagtc ttcaatcaat
      781 aactttgcat catttatttt tccattctca atcatatttt ctatacgaat aataaagtta
      841 tcttttaata atttaaattc ttcactttct gttatacttt cttctaattc acttatgaag
      901 atttttatta ttctattgta aaaagggtat tcatttaata atattctcat attatgtaga
      961 tattctaatt tttcattagg attatttatc ttccttttac catcaagtac atgcttaaat
     1021 tctaaagtga acttatctat gtcattacaa acaaacttta ttaattcaca atcactcaaa
     1081 ttgctatata tgtattttat gtatttataa gagtacatta tataaatata aaaaagttct
     1141 ttgtattttt tatcacttaa attaacactt tctaaaagaa ttttagatat aactttgtac
     1201 actcttatct tttctatttc tgatgtatta ggacaactaa ttatatattt atatattgat
     1261 acattaaaac ttctttctaa agctaaaata taccttaagt atttctctat ccaaacatat
     1321 tccatattgt aaaaaatgtc taataagtca actttttcct caaaagctat ttttataaca
     1381 cttgcataaa ctatgtcttt ttcttcattt agtatttttc tacatgtatc taatgataca
     1441 tgtttgttta ttcttatttt attcaaaaga ccataattac cttcaatatc acaaaggctt
     1501 ttatatatga gctctttctc atcatctttt atattattta taaagtcttc catactttta
     1561 taaaaactat ctctctcaac cttcgaattt ggaatctctc tataatagtt ttttatgtct
     1621 tcaaatctat ttaatttata taaagacata aataagctaa agtacacatt tttctttttt
     1681 tccacatctt caatattatc atactctgaa attactaaat catatttctc aagcttataa
     1741 taaattttta tcaatgtaat tatagcttca tctctaaatc ctacagcatc agtatctgaa
     1801 agcaaactat ttgcttgcgt actcatttca taattatcta gcaaataaat atatctttta
     1861 tatgaatcta tactattttc atacttccct aaatcaactt gtgcttgtgc aatatgataa
     1921 tatatatcaa tattatttct gtccttatta atatatttta agcataattt ttcacacttc
     1981 ttagcctttc ctaagtataa atatactttt gcaaggtttg ttatcaagta tccaggtact
     2041 atttctaatt ttgagtataa tttataagac ttttctagat aatctaaagc ttcttgatat
     2101 tttcctaata taataagatt tttccctatt tgataatttg tatatggaga tttctcgtct
     2161 tcttttaatt cctcaagtaa cagtttttca tttcttttta ctttatttat tcttatctct
     2221 tcatcttcaa acaaatatcc ataatgttca aaatcagcaa tattattaaa tataggattt
     2281 ttatacattg gttgttcatg aactttccca cagtatttaa aatcaaggtt tttgaaaaat
     2341 ctcaacactg aacaatatcc atataatttt ttatcaaatg aataaagatt ttttaatcgt
     2401 actgaggcag agttaaattt tttattaaga ccactttcaa aaaactttat aatatttgaa
     2461 taatcaatta atacttcatc tgcatctaat ataagtaacc attcgccctt agcgtaggat
     2521 atagatatat ttctcatact agaaaaatca tcattccaat tatgaaaata taccttttct
     2581 gtatattgtt ttgctattct tatagtatta tctgtagaac ctgtatcaac tattataagt
     2641 tcactctcta ttgaatttct caatactgat aacgatttta aagttttttc aagtatattt
     2701 tcttcgtttt tgaccatcat aactatacta agtaacatat atattttcct ttcaaaacta
     2761 tatttctaaa aaaagaaaag aataaagcaa agccttatcc tttctttttt attcaatata
     2821 ctaaatttat tctatcctaa taattgtaaa actccttgtg gttgttgatt agcttgagca
     2881 agcattgatt gtgatgcttg aactaatata ttcattttag ataagttaac catttctgaa
     2941 gcaacatctg tatctcttat tcttgattct gctgctgtaa cattctctaa agtattattt
     3001 aaattgtttt gagttgattc tagtctgttt tgttgtgctc ctaattttgc tcttgatgag
     3061 tttaagctct taagagctgc atctaagcta gataccatag ttttagctgc atctgttcct
     3121 gtagttccac ctgccttcat acttcctatt gaagcagttg ttataccagc tgaagccatt
     3181 atacttgcag tatttactaa ttgaattttt atctcattgt tattattact tgtacctgaa
     3241 acattagttc cataacttgc tccaacttgt aatcttattg ttgaagaagt tccatcaagt
     3301 agcttcttac cgttaaactc tgttgaactt gatattcttt caatctcatc ttttaattgt
     3361 gttaactcat cagctatctt ttctctttct tcagtattat taatttcatt tgcagattgt
     3421 aatgataaag ttctcattct ttgtagtata tttccagttt cttctaacga accttctgct
     3481 gtttgtacaa cagatatacc atcttgaacg tttcttcctg cttggtctaa accttttatt
     3541 tgagctctca ttttctcaga tattgcaagt ccagcagcat catcagcagc tctcttgatt
     3601 ctaaccccag aagataactt ctccatagac ttactttgtc catttacgtt tcttcccatt
     3661 tgattgtttg ctatcaaagc acttacattt gtattaactc tcattatttt accctcctta
     3721 gtatagttga catccatgtc atttctactt ttaacttttg tattaactat ccgtttatat
     3781 tatcggacaa gttaatctat aactttatac ttttttatta aaaatattta ctttttctct
     3841 atttgaacta taatacattg tgtttacctg tttagtaagc ttgtactcat gtaattcttt
     3901 ttttacttct gataattgtt cttgcaaagc atctcttatc ttagcatcaa tttcaaatag
     3961 ctccatacta tcataaagtt gtataaattc acttttatca acttcactta tactatttat
     4021 aatgttttgt ctctcatcta attgaaaact tatattttta tactccattt tttcaataaa
     4081 atttataatt tttaaagata tatctttata taaatttatt ttttcttcta acttgtccat
     4141 aattaattac cttgtgcttg tgagaaatag ttcatttgtg aagaatattt attcaacaaa
     4201 gactctaaag ctgaatattt agcataaagt cttttctctt tatcattcat tttttcaact
     4261 aacctactta tatttctttc ttgctctgca atttgttttg aatatacatt ttgactagca
     4321 gacgaagaat tttccatacc tgctttttta gtaagtttag aagctgaact tccagaatat
     4381 tcaaatacaa ttttttttaa tttttcaaac atacccttat catcataatt tgaagaatat
     4441 ccagctaaag ctttatatac cttgtcacta tcatctataa gagccttcga aaaagtatct
     4501 gcattaaatg atatttgtcc ttttttgtta taattctcat gctcactaat acccagtttt
     4561 cttaaagcaa taatagtatc tgcatctcca aaaaacatag actgaatatc ttcaacaaaa
     4621 gctcttaatt catcatcatt tctaagtatt ccttcctttg ccttcttttc ccacttttct
     4681 atttcttcag ttgtcatatc atctttttgt tcatctgtaa gaggtggata atcttttgat
     4741 tttttagtag taactaatcc ataaacctta tccatcaatg cattataatc atctacaaat
     4801 gctttcatat ttttagtagc ttcttcagtg tcagtaacag aaaccagttt agcacttcct
     4861 tttgaattta cattataatc aatgttatct attttaaatg aattctgttc tttacttatg
     4921 tgtgttactc ttccttggtc atcagttaca tcagcttcta aattttttcc atatactact
     4981 gcagatttag ttccactagc atcagttgta aatgtaatac cattatcact ggcaatttgt
     5041 ccagctaaac tatcatttcc aattactttt aaatcaatat ctgatgatga accagtctgt
     5101 tttcttgaga taatcaattc acctgtcatc tcactataag aagctcttat atcattatct
     5161 gcacaaaggg agtttatctt acttacaata gtacttgtag tatcttgttc agatattggt
     5221 atatttacac cacctatatt taaagaactt gctccactag catttggagg aaactgttga
     5281 acaatagggt cacttgaatt taacttaata gttacttttg ctggttcagc catttgagaa
     5341 actgcaaatt gataatttat tttttctgca ccagctgagc cttttgctac tataatactt
     5401 tcatcagaac tagttattct tgtagcagaa tatgcttttt tatttgttat actattagga
     5461 gagtctccat ttaaatactt gtcataaaga ttctttgact cttttataat atccctgtat
     5521 atttcttgtt tccatttaac tatttgttga tcttgtttcg ctttattaac tttttcctta
     5581 tctcttatca tactagcttc tattatacct tccatatcaa aatttcctga aaggcctgta
     5641 actcttactg gacttatact tgacatatgt ttatcccccc atcatgatta tagttatagt
     5701 ttattataca cttcatgcca tgtatctctt atttgttcta ctattggcaa tatatcatca
     5761 attattttaa tatctttatt tatatcagcc tttactaatt catgttttat gaattcataa
     5821 actttataca tatcctttgc ccattgtcct acactttggt ctaatgtgac cattaattct
     5881 gtaaatatat tttgaactct aacaagttca ttgtgtgctt ttaaatagtc tttatttaaa
     5941 agtgcaactt tagatatatt agtatacttg actgtaccat ctacaagcat taatagtaat
     6001 tcttcttttg ataacccatt tagttcattt aaatctaact ccatcattta tacctacttc
     6061 tttcttctct actttttcta tcagcctcat gccatgtatc tttaatttct tctattacag
     6121 gtaatacatt atctattatt tgtacatctt ttctgatatt tgcccttgaa agttcatatc
     6181 ttacaaaatc ataaactcta tacatatctt tcgcccattg acctgcattt tggtctaatg
     6241 taaccattag ttctgtaaat atatcttgaa ctcttataag ctctctatgt gcttttcgtg
     6301 tatttttatc tataatagct cctctagcta tttttgtata ttttactgca ccatcaacaa
     6361 gcataagcaa taattgttct tttgacgcca taaaaatagc attttgctta taagaattat
     6421 aaggattttc tccgtacata tattacttcc tccaagtgtt ttattttaat gattttaaga
     6481 tttttatatt tttatttgtg gcttttatat tttcattttt tactttttca tatatttctt
     6541 ttcttaagat acttatatta ttaggagcag atatagctaa ttttatgtta tttccatcta
     6601 ctcctacaac ttttacttct atgttatctc ctataagtac tgcttcatct ttttttctcg
     6661 aaattactag catccaccat cacctctcaa taatggatgc catattgaat attcctcatc
     6721 ctgtaaaatt atttgcattc cgcaattatt tcttacattt attactatag gagctttcat
     6781 gttcacagtt gattcttcaa gtgtttgacc aacagtgatt atattaagca acataatatc
     6841 atttggagat tttacttcaa gtttttctat tgtttcttga tttaggacta tttcatattc
     6901 cttaataatt tcaaatggtg atacaacaat aaatcctata caatcttctt cagtagaatt
     6961 aaattctttt aaaatatctt cattttctat atctaaaagc tcatactctt ttaagttttc
     7021 aaatcctagt attccttttt ttaatgtaat cttcatcata tctgcgttat tttactatat
     7081 ataatctaat atagatacat taaataactt tcctcctgtt tgtagtgaag cttgataagt
     7141 taattctgca gcctttaatt gaacaagctt ttccccataa tctatttcat cattttgcga
     7201 taaaacatcc tttaactgta tcaaaccttc atcatttttt tctttcatgg catctactgt
     7261 gtttgccttt gtaccatata tagacctaac atttactgta ttatctatta gattctccat
     7321 gtcacctaaa caagttgttg tcaactcttt tatatttgca tcacttggat ttgccagttt
     7381 cttcgatata ttatttaact tttcaaatgg atcagttttt gttggatcaa acccaagact
     7441 actcaactta acattataat ctacactgat tccttgagct acatctacac ttaaagaatc
     7501 atttaattta ttaagattta agctagtatt aaactttaaa cttactgagc catcagcatt
     7561 ctctacacac tcaacaggtg gtgtgcctgt atttgaacct gaaaatatgt acttaccttc
     7621 atgtgttgca tttaaagcat ttgctatttc tttcattttt tcattggttg aattactaat
     7681 agtttttatt tcgtcatctg aatatgaacc attcccactt gataccaaac ccttctttat
     7741 atctgctaat aaattaccaa gttggtctaa tgaagtatca gttgtttcta accaatctgc
     7801 tgtatcttta caattttcaa tataagtttc catagttttt atttcacttt ttgaattcaa
     7861 tatctttatc gccttatatg gattatctga tagtttattt atttctttcg ttgtgttgat
     7921 ttgtctattg accttgtcta atctttgcaa attatcttgc aatgaattta gataactact
     7981 ggacatcata cctgtactaa ctctcattat aaattcctcc ttttattaaa ttatcccatt
     8041 tattacaaca tccaatagtt gtactgctgt tgacataact tttgcactag cttgatagga
     8101 acgttgtact ttcatgagtt ctgtcatttc ttcatctaaa gatacacctg aaacactcat
     8161 tcgtgaatta tcaacattat ttataatttt acgttgattc acttcatctt gattaacttt
     8221 ttgagaatct aaacctaccg attctgcaaa agccttgtag tggtcactta ttgtgcttac
     8281 ttttccacca gctaccccaa tatctatttt ttcatccttt aacttagcaa tctttgaagc
     8341 ttcttcagta gtaagcttca attcatttga attacttttt attgctggat ttacttttat
     8401 aatatcttca ccagctacat ttgaggttat aaaaaaatct cttacagtta ttccagcaga
     8461 tgaattataa acatcattta tctgttttgc taatccatca gatacatctt ttaagtcgct
     8521 tttatatgta tcaattttac cgtgcatttc aaacaagcct tgtaaagttc ctgatatacc
     8581 tgttttccaa gttccatctg catttttcaa ctcattaagt tccaatttac catctgaaag
     8641 catattttta acatcactat tttcaaggtc taacttaaaa ctcaagttat ccaatattct
     8701 atctctttca tcaagcaaat catttggtgt agagccagaa ccagagataa tatcaataga
     8761 tttatttaag tcttcaagac tttttaacat gtcatttatt tgttttattt cagtatctat
     8821 attagaatct atatctcctt ttaacttgtc aagcttctta taactttcgc ttattaaagt
     8881 cgctaaatta gatgcatttt ctaccactag acttttttta ctatcactat ttatatcctt
     8941 tgataatgac tcccatcctt taaaaaaatc atctatacct gcagatattc catagtctga
     9001 tggttcattt agtatagttt ctatagtatt ataataatca tattttgaaa ctgtattccc
     9061 atacttatga gcttctgccc taaattggta atcataaaaa ctatttctag ctcttgttac
     9121 atctgttaca tggactccag tacctaattg tcctcttcca attgaattgc tcaaactagg
     9181 catatacatt ggactatttg tagcaaattc ggtcctttgc ctagaataac catctgtatc
     9241 taggttactt atattatgag aaattgtctg tattgatttt tggtgcgcat tcattcctga
     9301 attagctgta tttaaaatgg aaaataatcc tgccatattt ctcctcccaa tatctatttt
     9361 ctaatgtttc cataagaatt ataagttgta gtttgtttat caactggttt tatgaaatta
     9421 atcatttttt tcgtgaaaat aagttctttc tttattagag aatcatttga ttcttgttga
     9481 tttttagcta aagcaagaat atgctttaag tctatatata tttcttttaa atattcatca
     9541 ttactgtttt caacaacctc acttattgaa acatcatccc ctaaaagttg tcttcttttt
     9601 atttctatag atgcaagttc tctactaata tcttctagtt taggattcaa cctatttaaa
     9661 ttttctaagt ctttttcaat tatataatca aattgttctt ttagtaattc catcatatta
     9721 ctaattatct ttttttcttc atatattatg acttttaagt ctgtaatcat tactttatcc
     9781 tcgcatctcc tctatcattt ttttagcaat ttcttctggt ttaattttgt aagttccatt
     9841 ttctaatgca ctttttattt cttctatttt tttatttcta gccgcttcat cgctcatatt
     9901 tgatacactt gacatttcct ttgcaaattt agatagttct attttgtctg ttttatcaac
     9961 tttttgaact tgttgtacct tttttacatc cgatttgttc tttagataat tatttactac
    10021 aatatttgaa cttacacttt taatattcat aacctcacct ccaaaattac atccttatat
    10081 tttaattatc gctcaaagaa gaaaaatatt tatacgattc aaatagattt taaatttttt
    10141 ataaaaatac cccttgatta aaccaatata gaataatcta aggggatatt ttttaaagta
    10201 ttaatttacg tctgaatctt ctattttaga taatcttaca gataatgttt catttgtaat
    10261 aatactttct ccataagcaa acaatctgtc atttgcatat atgtctaatt tttcatctac
    10321 attcttattt agttttatta tagaaccttc ttttaaatct aatatatttt ttattgtttc
    10381 tgttatgttt cctatagaaa cagccacatt tactttagca ttaaaaacat tttgattttc
    10441 ctctataatc ccagtctttt cttctattaa ttgaccaaaa ttcaattcat atatatcttc
    10501 ttttttgcta ttcataaaac tattctcctt atgtaatctt atctatacat atctatcaat
    10561 aagccttgta tcatatcaac agtagaaaca atatctatat tttctttttc ttcactaagt
    10621 agtttactag tcatatcctc aagtttatta tcaataatct ttacattttt ataatattga
    10681 tgctcccaga aactactact tttatcaagt gaatacatat tatctacaac agattttagg
    10741 tattctttta ctgcattttt atatctacta atgtcaccat agttttgtgt aagtacaagc
    10801 ctctccccag ccttttttac accatctaaa ttccttttta tatcttctag tgaagaagat
    10861 tgttttacaa agtttaagct gtcagaaaac ttattatttt cctttgtctt tttaacttct
    10921 atacttctac ttggacttat atttagcttg tctatattta tacccatagt aaatcctacc
    10981 ctctataata tttcataact ttaggtatat aattttgtgt ctcctttggc attttataca
    11041 agtcagaaat tgattctacg cctctacttc tcattctacc aggtccacca ttatatgcca
    11101 tcaatcccat ttctatgcta cctccaaaca tatctatata ttccttaata tgtctagtac
    11161 cagcatctat attttgttct atgttaaaag ggtttgttac tcccaaatct ctgcaatttt
    11221 ctggcataag ttgcataaga cccttggcac cagcagaaga tacagtattt gggttaaaat
    11281 ctgattcaac cttaattatt gcctttataa gatttgcatc tacattatat ttctttgatg
    11341 cttgctctac agcattttca attctcgact ttacactttt atcttttgta tcaacttcaa
    11401 taaacttacc aactgtatta tttgctacac tatcaagttc attaaacagt ttttcagaat
    11461 tagatgataa attatatcca ttagaggttt gttgattatt ttgtgataat gctttcaaca
    11521 atgtcatcat aatcatatca aatccagatg aattattata tccacagttg cattgatttg
    11581 tggtattact accagataat tgggtcaatg ccagtatatt tattagtgaa cttatttcgt
    11641 tcaaaataaa actccccttt ctaaatttta taaactttaa agttatcctt atataattca
    11701 agatacttga gttcctataa aatcttcttg ttatagacta ctttgttaaa ttaatttctt
    11761 ttatattcat taaaacttta gatttagttt cactacatta tgattatcgg ataaataaaa
    11821 aaaatattta tactaaa
//
LOCUS       DACXMZ010000113        11826 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.114, whole genome shotgun sequence.
ACCESSION   DACXMZ010000113 DACXMZ010000000
VERSION     DACXMZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.114"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            260..3358
                     /locus_tag="JD016_11875"
     CDS             260..3358
                     /locus_tag="JD016_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HAU5000331.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            3481..4767
                     /locus_tag="JD016_11880"
     CDS             3481..4767
                     /locus_tag="JD016_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAU5000332.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(4977..6083)
                     /locus_tag="JD016_11885"
     CDS             complement(4977..6083)
                     /locus_tag="JD016_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAU5000333.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGKKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(6118..7659)
                     /locus_tag="JD016_11890"
     CDS             complement(6118..7659)
                     /locus_tag="JD016_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HAU5000334.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            complement(8021..8767)
                     /locus_tag="JD016_11895"
     CDS             complement(8021..8767)
                     /locus_tag="JD016_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAU5000335.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     SKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(8979..10289)
                     /locus_tag="JD016_11900"
     CDS             complement(8979..10289)
                     /locus_tag="JD016_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAU5000336.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            complement(10302..10772)
                     /locus_tag="JD016_11905"
     CDS             complement(10302..10772)
                     /locus_tag="JD016_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000337.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(10776..11627)
                     /locus_tag="JD016_11910"
     CDS             complement(10776..11627)
                     /locus_tag="JD016_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HAU5000338.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
BASE COUNT         4331 a         1655 c         1787 g         4053 t
ORIGIN      
        1 gaggtggaga tttgtcattt atatgagaat taattataaa cacataatga gataattaat
       61 tataaatata ttaaattaag tattacaaaa ctctattgta cgattgacac aataataatt
      121 ctatttacta aaaaatagaa cctcattaag gtactatttt aaaacataaa gaagtataat
      181 atcattgcca aatatatagc aaaattttaa aagctttaaa aattcaatct ggaaaatgaa
      241 taaaaggaga tatatataaa tggatgtaat aaatttaata gatgagttgg atgaaattgt
      301 atatgtttct tctttagata catatgaact tttatatatt aatgaaattg ggaaaaaggc
      361 aatgggactt aatgatatat cccatttaaa atgttataaa gcattacagg ggaaagattc
      421 tccctgcgaa ttttgtacaa actctatttt aaaagaagat gaatattaca tatgggaaaa
      481 tacgaatact cgaacaaata gacattttat attaaaggac aagttaatta aatgggaggg
      541 caaacatgca cgattagaaa tagctcttga tattacagaa aaggaagata taagtaagtc
      601 aatagcagaa aaattagaga taaaggagtt attagttgga tgtattaaac atcttataac
      661 agaatctaat ttttctgatg ctgtaaatct tgtattaacc aatattggta aatttcataa
      721 agctgataga gcatatgttt ttgaagtatc agaagataga acaaaagtaa ctaatacata
      781 tgaatggtgc aatgaaggtg ttgatgaaca aaaacaggca ctaacaaatc ttgatattaa
      841 tgaattaaag aattggtttt tattattcga acaaaaaggt tttgtaataa ttgatgatat
      901 agaaaatata aaagaagaac agccaataga atatgaaatt ttaaaacctc aaaaaattga
      961 aagtttaatg acatcttcac taaaacaaga tgataaagta gtagggttta ttggagttga
     1021 taatccacat aaagctgttg gagatacctc tcttttgaca tccctatctt attttttatt
     1081 aactgagatg aaaaaaagaa aagtagaaaa taaattaaat tatatgagct actatgactt
     1141 cttgactggt gcatataata gaaataaata tattaagtat atagataatt ttcaaaaagg
     1201 aaaagttaaa tccataggag ttgtatttgt agatataaat gggttaaaag atattaatga
     1261 taactttgga catgctaatg gcgattctgc tattgtgtca gcatgtgtaa taataaaaaa
     1321 atactttcat tcttataatg tttaccgtat tggaggagat gagtttgttg tattatgtga
     1381 agatatatca aaacaactat ttgaagaatt agtgagttgg ttaagtgaag aatttaagga
     1441 tgagatgata tatagtgtag cagtgggcag tatttggagt gacgataata ttgatataca
     1501 taagctaatt aaagttgctg atgagagaat gtaccaagat aagaagaggt attataataa
     1561 gaacaaactt agaaataatg aaatgaaagt aattagtgac ttagctgatt cgacgaataa
     1621 tattttattt aaacataaag agttagttga taaggtttat tttgatgatt taatgataaa
     1681 agggtattct aaagcttatg aaaaattagc ttataataca tatgatataa ttttatctat
     1741 ttgcattgat acgaatttaa ttactccaat ttatcaatca gagagcaata tttttagttt
     1801 tcctataaag gaaaatcttg aaaaaacttt ttcatacata aaaaaacatt taattcatcc
     1861 tgatgatata atattattta atgataacca ccttctaaaa tctcttgata gtcttaataa
     1921 tagcaagcaa gacgatttag aactaaattt tgagtataga cgtttaggaa tagataaaaa
     1981 gtactattgg gcattaattt atatgttaaa gattagtagt aaaaataagt taggtgagaa
     2041 acaaacacat attttagttg ctataaaaaa tatagattat cttatgcaac tacataaaaa
     2101 ccaaaaagat acattaacag gactatacaa ttatgaaagt ttttgtgaac aaataagttt
     2161 aatgcttaaa agatatcctg acaagcgtta tgcaattata atgatggata tagataagtt
     2221 caaggtaatt aatgacattt atggagttag aggaggagat aaagctttaa tatatgttgc
     2281 agagttgatt caaaaaaaca ttggttctaa tggtatatgt tgtcgtatgt atgcagatgt
     2341 attttgcatt ttttatgaga gttactctga tagagatatt cataaggtaa tttataagat
     2401 taaaaatagc ttaaatagaa aaaaatttga atatatgtta gtaccatgtt ttggggttta
     2461 tagaattttg gataatcata ttccaattac aaatgcgtgt gaaatggcag gatatgctca
     2521 caaaaaagta aagaaacaaa gtatcaataa ctatatattt tatgatgata ctataagaaa
     2581 tgatgcactt gaagaaaaac agatggaaag ggaaatggaa tacgcacttg aaaatgggca
     2641 attccatgta tatttacaac ctaagtataa tcttaatgca aataaaattg ttggtgcaga
     2701 agcattggtt agatgggttc atccagaaaa aggtataata cctccaatta aatttatttc
     2761 actatttgag aagaatgggt ttattatcag attagatatg tatgtatggg aacaagtatg
     2821 ttctttgatt agaaaatgga tagatttagg tgaagagcca gttcctatct ctgtaaatgt
     2881 atcaagatta catttgcata atccacattt taaagaaatt gttttaagtt taataaataa
     2941 atatagtata ccaaaatctt ttttagaatt agagcttaca gaaagtttat ttattaaaaa
     3001 tataaaatta tttactaaga taatagccga ccttagaatg gatggtattg ttttaaatat
     3061 ggatgacttt ggttcagcat attcttcatt aaatatgtta aaaaatatta atattgatac
     3121 attaaagata gattgtggtt tctttaatga ggaaggaatc acagacagag aaaaaatagt
     3181 tttaaagcac actatagcca tggcaaaaga tttagatatg gatgtagtgg cagaaggtgt
     3241 agagacaaaa gaacaagctg aatttttagt gagtttagat tgtgtggtaa ttcaaggtta
     3301 ttattattgt aagcctattc caagtgctga ttttgagaag ttactttatc aaaaataagc
     3361 taattattcc attctaaatg taggtaggat ggagtagttc aaaataatta aatagaagta
     3421 gaaaatagtg aggatataat ttttaaaaag tataatattt atggattagg agcgaagttt
     3481 atgatagtgg gtattatagg tccttcagac tctgggttta agataaagga tgatttaaaa
     3541 cagatagaca gtgatttaaa gactaaaata tatgtaagag aaaaagtagt agatacaata
     3601 gaagttattt ctaaatgtga agacgaatgt gatgctatta tatttacagg atgtgcagtt
     3661 tatgaattta taaaatctaa gtatgagata agtaagccac attcatttgt accgagaagt
     3721 gggacgagta taatgaaggc attttgggca ataaaaagtg caaatataaa attagataaa
     3781 ttcagtatag atgtagttga tagatatgtt gtggaagatg cacttaaaga atttgatatt
     3841 aaagcaacga gtgtattttg taatccattt tctttggaag taggagagtc tgaacttgta
     3901 gaatggcata taaaattatt tgaagaaaat aagacggata taatgttaac tggttttggt
     3961 gcagtatata atgaattaaa agaaagaggc tatcctgtat ttcgtttaca agctactata
     4021 caattaatca aagaaagtta tgacaaggta aagagtgaat atgctctaag taaggcaaga
     4081 ttttctcaaa ttgctgttga aatattgagt ttaatagatt ataaagagaa gatagataac
     4141 tattattctg acatgataaa aaaatcagat atggataagc ttgtagtaaa ctatgtaaga
     4201 agcattcaag gttccttatt tttacttgga agaaatgatt atgttgtatt tgttcataaa
     4261 ggggtagctg ataatgagta taattatgat aagctattca atctaaagag agagataaaa
     4321 gacatgggct tttctcttag tataggaatt ggtactggag taactgcgta tcaggctgaa
     4381 aataatgcac ataaagcatt aagacactcc atagattcaa aagaaattgg tatatatttg
     4441 gtagatgaag atgaaaatat aagaggacct ttagcttccg aaaatgaatt aaattattct
     4501 ttgatgctat ctgatgaaaa ggtaatcgaa atatctaaaa agactggaat gagttgtgag
     4561 tcagttgcaa aaattattgc tataaatgag aatagaaaaa gtaagattta tgattcaaaa
     4621 gagttagcag agtatctaaa tgtaagtgaa agaagtgcaa gacgtatatt aaataaaata
     4681 gtaaatgcag gtttaggtcg aatatgtgca aaagagacta gtattggtgg aggaagacca
     4741 aaaaatatta ctgaaatact gttttaagat attttataaa actgtataaa tcattatatt
     4801 gtataaaatt ctacaacaaa ttactatata aagtattaaa gcttattagg aaaaaaatta
     4861 gaacaaatta ttataaaaat actaaaacct atgtaaaaca actctaatat ataacttata
     4921 aagttgaaga tacataggtt tttattttta taatttaaag agttctatta tgatatttat
     4981 aatttaatca tatgataaac tattttggca gtatcatata attcgtctat atttacatat
     5041 tcattaactg tgtgtacatc atacatacct agacttaata tagcacaatt atatccaaga
     5101 ttagctaata tatttgcgtc acttccacca ccaattacca ttggatttgg aacaacacca
     5161 acttgtctta ttgcttcttc acttaacttg aaaacatgac tatctctact aagttcaaaa
     5221 ctagggtaag acatattatg ttcaaaagta taagtagtat taaatttaga tgcagcatcc
     5281 ttacaacatt tttccatatg attaagctca tactctaaag tttcaggtat atgagaccta
     5341 atttcagcag tcaatgtaac cttatcagtt actatatttg tagctccacc accttctatt
     5401 ttacctatat ttgaagtagt caatgaatct attctgccta tgtgcatgtt gcttattgca
     5461 tgagaggcta ctaatatagc atttaaccct ttttcaggtt ctataccagc atgtgctttc
     5521 tttccatgaa aagagatttt tatagattgt tgagcaggag ctttgtatgc tatcgaacca
     5581 atagctccac cagaatctaa aatcaccata tctttacaag gcaagttgtc aggattaaag
     5641 tttttgacac catgcatacc agcttcctca cagattgtaa aacaaagata aatatctcta
     5701 tgtggtattt tttctgtaat aacatgttct aaagcttcaa gtatagcagc tattccagct
     5761 ttatcatctg caccaagtgt agtagttcca tcacttctaa ctacattacc atctagtatt
     5821 ggttttacac ctaaacaagg ttgaacttgg tccatgtgtg cacataaaca aagtggtcta
     5881 cttccttctt caccttttat ataggcaatg acattacctg tattaccacc atatttttta
     5941 cctgcatcat ctataatggc atctatattt ctttctttta agtagtttac caaccactta
     6001 gacatttcta attcttgatt agatgggcta tcaattctaa ccatatccat aaaattattt
     6061 ataagtctat ttttatctaa cataaattat ttctcctttt agaaagattt aaataaatta
     6121 catgctatat gaaggaagta ttccaggtcc tagtggaata ttaaatacca taaacaatat
     6181 taatagtata gtccacacta ttaagaatga tatagagtat ggtatcatat tagcaattac
     6241 agtacccata ccagcatctt tatcgtattt tcttgtgaat gctagtataa tagggaagta
     6301 cggaaataat gggctaagtg gatttgttat acaatcacct atacgataag ccatctgagt
     6361 aagtgctgga ttgtatccaa ccatcataaa cattggtaca aatataggtg caagaatagc
     6421 ccactttgca gatgcactac ctataaataa atttataaaa catgataata taataaatcc
     6481 tatcaacaat aaaggtccat ttattcctgc tgattttaaa aattctccgc cctttacagc
     6541 tagtatcaga cctaaattac tatttgtgaa taattgcaaa aattgtgaag cagcaaatgc
     6601 tagtattata tatccaccca tatcactcat tgcactagcc attatagaga ctacatcctt
     6661 atccttcttt attttaccta tagtttttcc gtatacaaca ccaggtatga aaaatgctag
     6721 tgctattata ggtaccatac ctttcataag aggagcattg tttgatagta aatctcctgt
     6781 ttctgggtca gctaagaatg gttttttacc gattacacta agagctacta taatagccac
     6841 atatattaat atacttatac cagctttttt aagccctttc ttttctacat cactaagact
     6901 tccatcaaca tctagttctg catttccttc atatttacca aatctaggag ctacaatttt
     6961 ttctgttacc catgttgcca aaagtattag aacaaaagtt gatgctatca taaaaaataa
     7021 gttcattgtt gcattggctt gaaatccaga atcaatcatt tgagctgctg gaactgtaaa
     7081 acttgcagca agtatatcat tgacacttat cataatgttt gcggcaaatc ctagacagac
     7141 tgctgcatat ccagaaaata aacctataag tggatgtcta ccaacaccaa gatatacaag
     7201 tgcagctaat ggtggaaata atattgtacc tgcatctgaa gctatattgg caagcatacc
     7261 tatagtcatt acaacaagag ttactcttgt ttcaggaatc ttgcttactg atgctttgat
     7321 tgttgctgtc ataagtcctg ttttgtcaca tacaccagca cctatcatag ttactagaac
     7381 tagacccaaa ggagcaaacc cttggaaatt gctgactatg tttcctaaaa gaatttgaag
     7441 ctgttctaca gtcaataaat ttacagcggt tatagtttct ccagtaccag gatgaactac
     7501 tgatataccc atagaaccaa ctagactaga tagtataagt aaaagtacac ataaccctaa
     7561 aaatattgta actgggtctg gtagtttatt accaacagtt tctattctgt ttaatgtcct
     7621 gtcaaaaaag tttttcttct taactacgtt atcattcata atatcctccc taatgtttaa
     7681 aaatataatt aaaaaataag tattgtggtt aatcttacaa tttaaattct gcttgtcgat
     7741 ttaagacgtg tccacaatat gtcctaaaaa aagtataaaa taaacttttt cctgtgtcaa
     7801 tgcagttttc tgaattttct ggttgagtgg tgttagttaa atatgcttta agtagattaa
     7861 tgacgaattt tatctaatgt atttaaataa catatgcgct ttaaaattaa ttttaaaaca
     7921 aaattttatg aaaagctagt ggaagattaa gttattaaaa taagtagcgt agtaaagtgt
     7981 tagatttttt aattaatcaa ataaatttac tttgtatttt ctacaaaaat ctgtgaatat
     8041 cttttgaatt atgtagagga tataaattta ttttatttac atagattaga ttttgttcta
     8101 atttactgta ttcaaatggt tttccatgtc caggataaat cattttaggt tttgtattga
     8161 taattgtttt ccaagattga ataaattcta gtttgctttc tgcaaatatg gtaattttgt
     8221 gagtacttgg aaatccattc attgcagaat ctccacaaaa taaaacacca tcatcaatta
     8281 gtagtgaaat tgaatcagca gtatgtcctg gtgtatctat aatttttcca tttagttctt
     8341 tttcaatttc tttacgattc ttgtcagaca caagaataca ccttttttca aattcttgtt
     8401 taagtggaga aaaactatgc tcaccttttc caaatagttt cataatttgg caaaagagaa
     8461 aggcagtacg gctggtacaa ccaccaataa aagagttttg acctttatat agaatttcta
     8521 aagctttatc atgcataaca atctttgtat taggagattc gtctagaatc tgatttagaa
     8581 aaccaacatg gtcatcatga gcatgagtta gaaatatgta tcgaatatct ttgcttttta
     8641 tgttgaattt agaaattcgt tttttgaaat gtttaaaacc attttcatat ccagtatcaa
     8701 tcaagacaaa gccatcttga atagggtaca accatgtatt tataattcga tttcctgcat
     8761 taaacatttt ttcaccacct tgaattagaa tataaattta tattaattag aaagaatata
     8821 gcatagttct actgatattt caacgtatct ctaaaagttt taaggaggat aatatatttt
     8881 agtttaatct aaaaactaaa agaaaagaag tatactgtta caaaaacaaa gagtggtata
     8941 acttttacag tagataccac cctattataa aaatagactt ataaagtttt atcgtgtttt
     9001 tctaagaatg ttagatattc ttctttagtc ataactggag taaaggtatc taatatatct
     9061 ttagcatcct tagcattatc aaaaagaagg tctacaactg taagtgccat tgctttagca
     9121 ggaattatgt aagctaaatc ttcatcaact atttcaaaat ctcttgtatg aagagctcct
     9181 tttacaccac caatcatagg gtgaagagta ggcattatat gagacacatc tccaaaatca
     9241 aaagagcctg taaaatctcc accatcaata acttttcctt ttagacctaa atcttcaaga
     9301 ttgtttttaa ataaattatc aagacttttg tatcgtaata ttggaagata tccaggaatt
     9361 tcagttatct caacatcagc tccaactgcc attccaccag ccatcaaagc cttatttatt
     9421 ctttcattag catcaatcat accatttata gttctagctc taacatatga ttccatatga
     9481 acatcagcag gaacaacatt tactatatct ccaccttttg tgatgatagg gtggaatcta
     9541 actctttcag actctttaaa agtttctcta agtgcattca cgttgtttat agcaagcatt
     9601 gctgcattta aagcatttat tccatcatgt ggagcagaac cagcatgaga ttctttacct
     9661 ataaatttaa ccttttttcc aacaaatcca ttactctcag gaccaactag agccttatct
     9721 tctcctaaat cagaagcatg aaacatcata gatatgtcta tatcatcaaa ataacctctt
     9781 tttattaatt cttgttttcc accaaaataa gttatttttc cttctttttt taattgctct
     9841 ctgtattcca actcaataaa ctcttcagca ggagtagcca taaaagatac tttaccatct
     9901 aaatgttcta aaacgccact ttttactaaa ccaacagcag cacctaacat tccagctatt
     9961 tgaatattat gtccacaagt atgagaagca cctatttcat tagcatcttt atgttcttta
    10021 catgatatac catccaattc tccaagtata gatatatgag gacctttctt gctagaattt
    10081 gcatctgctt tgcatcctgt aactgctata tttttttcta cagataaatt taattcttgc
    10141 tcaaaaaagt ttgaaacagc ttctgtagtt ttaaattctt tatatccata ttctggattt
    10201 ttgtatattc cttgacctat ttttaatatt ttttctctat tttcatctat agtttttatt
    10261 actagagatt ttaattgttc tttattcata ataaattcct cctatatttt accttgaata
    10321 cttaaaacta tatgagctat actcgcacat gcaaagaata tggttgtaaa tacaataagt
    10381 gaaattataa ctattttcca tgacatttct tttaaagctt ctattttgtt tccaacagaa
    10441 attcctgcaa aagctagtag tggagtagtt atagccataa agttaatatt attagtgtta
    10501 gttaagaata cttcagatgt tggactatat ggcatactaa gtaaaaggcc gattatagtt
    10561 gcaaatccaa aagctggaaa gattgattta ggaaataaat cttttataat catagcagcc
    10621 atagcagcta atattagcat caccatacca ggaagagctg taactataga tgtaccaacg
    10681 ctaattcttt gtcctataag aattataact ccaataacag acataaccaa taattgatat
    10741 gaacttttct ttaaatttaa attagcaaac atttattagc actctccttc tgtagattta
    10801 atttctttag tttttttatt tctcgaaaat tttggttcta aaatcttata cataaaatta
    10861 gtaaatggta aggatacaaa aactaagaat ataattcctg ttatactaga catcatattg
    10921 cttgttgcag cataggcaag tattgtatct gccatttcag gtggtaccat ggtagataat
    10981 gaaccagcag ctgctgtcat catacttcca ctaccaacac cacaggccat tcccaaagca
    11041 tatgggtgaa gaccactata aatagatatt gaacctagta cactaaagta tatagtacca
    11101 agaactgttc ccattaaata tgtaccaagt acaccactac cttctggtga gtttatccca
    11161 tatttttctg atataactcc taaagaaggt tcacgactta tacttgcagt agctccaaca
    11221 gcttctcttt tcattccaaa cattaatgca agaggtaatg caacgattgg tgcaagtata
    11281 tgaccaaatt cttgagcgat aaatgctggt ccagcctgaa ttattttgtc aatgtttggt
    11341 ccaactgtag taccatattt tataccaagc attactagtg caattccaac aacttcacca
    11401 gcaatattta tttctttttc tccaaccact tttttaagtg ctttaatttt tttacctaat
    11461 aaatctggtg ttattataag tccaattata actgcataaa gcatagggaa cagtactata
    11521 gaggcttttc caatagctat agttctttgc cctataaatt cacatattac taccaagatt
    11581 aagcaagtta aaaatacttg aataaaatgt tttttattaa tttccatgat ttcctcccca
    11641 aaagaataaa tattttactt atctgaaatg agaaaatacg ttttcttaag tataatacgt
    11701 tttcctaaat aaaatgaaaa aactatttca aataagtttc agaaatgtca ataacatgtc
    11761 cttaattaag gataaaacaa aaatttaaca ttgtcaacga aaaatatcta aaatattaca
    11821 tatttt
//
LOCUS       DACXMZ010000114        11777 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.115, whole genome shotgun sequence.
ACCESSION   DACXMZ010000114 DACXMZ010000000
VERSION     DACXMZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.115"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            304..825
                     /locus_tag="JD016_11915"
     CDS             304..825
                     /locus_tag="JD016_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAU5000339.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            1192..2367
                     /locus_tag="JD016_11920"
     CDS             1192..2367
                     /locus_tag="JD016_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAU5000340.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            2401..3939
                     /locus_tag="JD016_11925"
     CDS             2401..3939
                     /locus_tag="JD016_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HAU5000341.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            3971..5173
                     /locus_tag="JD016_11930"
     CDS             3971..5173
                     /locus_tag="JD016_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAU5000342.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(5340..5939)
                     /locus_tag="JD016_11935"
     CDS             complement(5340..5939)
                     /locus_tag="JD016_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAU5000343.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            6109..7467
                     /locus_tag="JD016_11940"
     CDS             6109..7467
                     /locus_tag="JD016_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000344.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            7460..8092
                     /locus_tag="JD016_11945"
     CDS             7460..8092
                     /locus_tag="JD016_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAU5000345.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            8421..9578
                     /locus_tag="JD016_11950"
     CDS             8421..9578
                     /locus_tag="JD016_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAU5000346.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILLGTIAESKKNDPCDICYGK"
     gene            complement(9725..10360)
                     /gene="lexA"
                     /locus_tag="JD016_11955"
     CDS             complement(9725..10360)
                     /gene="lexA"
                     /locus_tag="JD016_11955"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAU5000347.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(10464..11591)
                     /locus_tag="JD016_11960"
     CDS             complement(10464..11591)
                     /locus_tag="JD016_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAU5000348.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
BASE COUNT         4419 a         1446 c         1909 g         4003 t
ORIGIN      
        1 aatactattt tactgataat cttttcctta tcactaatct tacattttaa aatattattt
       61 ttactcgtaa tattatcata agaatttgtt tcttatttaa aactaattag aattataaag
      121 tttattgcca tgttattaat aaaataaatc cagttaaatc gagtgctagt taaagtaata
      181 agaaactttt acaatggttt cctttatcga ttgaaataaa agttaatatg gtataatatc
      241 taaagagaga atcttatcaa ttagaatatg catagaaaca agtggcttaa aaggagacgt
      301 tatatggaag agatagtaaa gaaggaacaa gcaagtatga atgatatttt tttgtatctt
      361 aaagctaaat caaactcatt atataaattt gtaatattat ataatgagtt tatcaataaa
      421 cctaaagatt atggaactgg ggaacttata aacatggttg aagtacatat gctaacagcg
      481 attgaagaaa atccaggaat aagtccaact cagttagcta aaatatggaa cagaactaaa
      541 ggtgcaatat ctcaaactat atctaaattg gagaaaaagg gttatataac taaagaaaag
      601 aaagaaggaa atgcaaaaac tatattacta tatccaacca catcaggagt agagttaagt
      661 aaggcacata aactatatga caccatagat gttacaagaa catttgaata cttaatcaag
      721 gagtgtacat tagaggagat agatagtttt tataaagtaa ttgatttgtt tattaattta
      781 ttagaagaat ctccgcagga aaataatgat tctgaaaaag tgtagctgag attgaagttg
      841 aataaagaga aatagatatt atagaagtag tacttaagta cacattatat aaaaatataa
      901 taggtatagg attaaaaagt ttagtggttt aaagtttaac cactaaactt ttttaatgtc
      961 tataatctac tattatttaa tttactaaaa ataaaaaaac tcattatatt aagataaaaa
     1021 tagaaaaata ctataatata tcagtttaat acagaaaaaa gttaaaaaat ttgagaaaaa
     1081 taaaaatgaa aacgttttat attgacatag agaaaaagta gtgataccat ttaaatatag
     1141 tttagtagct aaactatatt taaattataa taactatttt agggggatgt tatgaattta
     1201 aaacaattaa gtgaaaaata cgaagaatat gtaattcaaa tgagaagaga atttcataag
     1261 tatccagaat tatcttttaa agaaataaga acaacccaaa gaataaggga agaattacaa
     1321 aaaatagggg tcccttttgt atctattgaa ccaaatatcg taatagctac tatacaaggc
     1381 aaaaatgaag gtaagacaat tgcaattaga ggagatatag atgcgcttcc aatgcaagaa
     1441 gattcagatg ttgaatacaa atcagaatat gatggaataa tgcattcttg tggacatgat
     1501 gctcatggag ctatgttact aggacttgca catatgctaa atgatataag aaaagattta
     1561 aaaggtagag tattattagt ttttcaagca gctgaagaag ttggtggtgg acacaaggag
     1621 ataatacagt actttaagga aaatggagga ccagatagat taatagctac tcatgtttgg
     1681 tccgatattg aagctggtaa aatttctgtt gaaccaggac ctcgtatggc tggtgggtct
     1741 ggttggagaa tagacataac aggtagagga ggtcatggtt ctagaccaga tcaaagcata
     1801 gatcctataa agccagcttg tgatatagtt ttaaaagtat cttctatacc agttaatcat
     1861 ataagtgtac tagaacaatg tgttgtacat gcatgtgcaa tacatggagg aactacaata
     1921 ggaaatgtta ttccaaacaa tgcagaggta ataggtggat atagatattt tactgaagaa
     1981 tcaggcaaaa aagtgtttga cattataaag caaatgagta atggtgttgg acaagtttat
     2041 ggagtggatg tgaaggttac tcatactgga ggcataggac ctgttatcaa tgatgaagaa
     2101 gcagttaaga tggctaaaga aatagtttct aatatagatg gattggagtt agattcttat
     2161 gagccaatat gtgcatcaga ttgttatggt gaaatattaa aagagtattc tggcttttat
     2221 tgttatctag gggtaggtaa caaagaaaaa ggaataatat atgcacaaca ccatcctaag
     2281 tacaatatag atgaagatac attaaagtta ggttgcgaat ttatggctaa atatgcagtt
     2341 gaatttttaa ataaaaataa taactaaaat aaatcgaatt ttataaatgg ggggatgaaa
     2401 atgcaaggtg cattaaagga aaataagaaa aaaagtggtg gaggattttt aggatggatt
     2461 gaaaaagttg ggaataaaat gccacatcct ctagcattat ttacatatat tacattgata
     2521 gtagtagcat tatctgcaat agcaagcttt ttaggttttt cagcaacaag tcctgctaca
     2581 ggaaagttaa taaaagttat gaacttaata agtgcaaaag gtttagtact atttatgcaa
     2641 gagtttgtta agaacttcca aaacttccct gtgcttggag tagttttagt tatggcagtt
     2701 gccacaggag tttgtgaaaa gacaggattt ttttcaactg ccataaaaat gagtttatca
     2761 aaagttaagg ggaatgcggt tgtatttata atagctttta taggagtatt tgctaatcaa
     2821 gctggtgatg ctgcttttgt attagtacca actcttgcag gagcaatatt ttatggatta
     2881 aatagaaatc cattagctgg tatattctgt ggatttgcat cagttggtgg tggatttgca
     2941 acagctgtaa ttcctggggg atgggacgtt acgctaacgc caataactgt atcagctgct
     3001 caaactattc aacctgcatt tgatatgaca ttattagcta gttattatgc actatttgta
     3061 tctgcgttta tagtagctac agtatcaaca atagtaacgg ttaaatttat agaacctaaa
     3121 ttaggtaagt atactggtaa accagaaggt atagatgata atcaaggttt tgaggtcaca
     3181 aaagaacaag caaaagcagc aaagttagca ggttttacag ttttagctta tgtagcgtta
     3241 ttaattgctt tgtgtatacc agctaatagt tttttaagaa gtccagaagg ttctttaata
     3301 tttggagcac cacttatgtc ttgcttacag ttttttatag taatactatt cttcttgcca
     3361 gggattgtgt atggaatgag ggttggtaaa atcaagactg taaaagactt aaatgatata
     3421 ttatgtcaat caatggcagc aatggcaccg tttatcgtac ttgcaatagt tataggtcaa
     3481 ttcttaacgc tattctcagt gtctaattta gcacaggtac tagctataaa aggtggagca
     3541 ttactcaact cattgccact accaccacaa gctataatac tgctattctt agtactagta
     3601 gcgtttataa atatatttgt tataagcgga agtacaaaat ggatgatatt tggaccagta
     3661 tttgttccaa tgttaatgca attaaacata catccagcat ttactcagtt tgtatataga
     3721 ttaggagatt ctgtaaccaa tcatttatca ccattaaatg cattctttat aatactttta
     3781 gcaacagtcc aaaaatacga taaaaatgca ggtatgggaa ctatattctc agctatgata
     3841 ccttacacta taggttactt tgtagtactt gcagttttag tagtagtttg gatgacaata
     3901 ggtataccag taggaatagg tggacaagtt tggttataaa acatatttaa taattttggg
     3961 aggaaagaaa atggaaacaa ctgaaataag atacaatttt gatgaagtaa tagatagaag
     4021 tggaacaaac tgtcttaaat atgatcttaa gagtcaattt atgccagaat ctccagaaga
     4081 tgcactacca ctatgggtag cagatatgga ttttgcatgt gcccctgaga taattaatgc
     4141 aatgcatgaa agaatagaca gaaaaatatt tggatatagc tctcaacaaa ctgatgatta
     4201 ttatgaagct gtatgtggat ggtttaagag aagatttgat tggaaaatag aaaaagagaa
     4261 tatattcttt agcccagggg ttgtgcctgc gttaggattt ttcacacaaa tattaacaca
     4321 aaagggtgat ggtatcataa tagaaaagcc tgtatactat ccttttgaag ataaaataac
     4381 taataatgaa agaaaagtag ttaatagtcc tttaaagttt gaagatggtc attattctat
     4441 ggattatgat ggatttgaag aattagctaa agatgaaaat aataaggtgt ttatattatg
     4501 tagtcctcat aatccagttg gtagagtatg gaaaaaagaa gagcttagaa aaatagtaga
     4561 catatgtaag aaatatgatt tgtggataat atctgatgaa atacattgtg atattattcg
     4621 taaaggtgaa aagcatatac cattagaagt agcatgtcct gattataaag atagaataat
     4681 aacttgtaca gctccaagta agagttttaa cttagctggt atgcaattat caaatatagt
     4741 aatcaataat aaggaattac aaaaaaaatg gatgcttgaa acagattcta aattagctat
     4801 attcctacct aatccattcg caatagttgc tactaaagtt gcttataatg attgtgaaga
     4861 ttggatgaat caagtaaatg agtatataga tggtaatatt aaatatgtag aagattttgt
     4921 taatgagtat atgcctaaag taaagataat aaaatctcaa ggaacttact tagtttggtt
     4981 agactttaga gggtatggat tagatgcaaa acagcttgaa tatgtaatgc ttaacaaggc
     5041 gaaggtattg ctagatgaag gatacatatt cggagaagaa ggagcaggat ttgaaagaat
     5101 aaatgttgct tcacctagaa caattataga agagtgtatg aatagaataa aatgtgcatt
     5161 tgaaggatta taatagtaat atctaaataa tatatttgtg tattttaaaa aaagactatg
     5221 ctaaaaaaat atttcaaagc atagtctttt aaagttttat gatgagttta aaaaatttca
     5281 aaacgttact attcacttat atgtttatat tagaaataat tccacaatat actcattttc
     5341 taaagttgca cttttataca ccttgcttca ccaatatcat gtgtaaccaa aatagtagac
     5401 ttattctcat tcttaataat actataaaca tcatcacaaa ctaataatct agtctgataa
     5461 tcaagagctg aaaaaggttc atctaaaagt agaatatctg gatttacaga aagtgttcta
     5521 ataagcgcaa ctctttgtct catacctcca gataattctt ttggatacat acttctaaaa
     5581 tcccataatc cataagtctt taaaaggctt tcaactctat ctaaagattt tttatctttt
     5641 ttgccttgaa tttccaatcc aatagttata ttatccatta tatttctcca ttcaagcaaa
     5701 ttatcttttt gaaacatata tccaatattt ccagtaatct ttatatcacc actagtcggt
     5761 tttaaaagat tagtaagaat atttaatatt gtagatttac cacttccaga aggaccaagc
     5821 aaagttaata tttctccttc tttcaagtta aaattgacat cctttaatac ttcaagctcg
     5881 ccttccttag tgtagaagtt catatttata tttttaactt ctactatgtt gtcattcatt
     5941 atgccacccc ctataacttt tagctcgtat atattatgtt attaacaata tgacctattt
     6001 gtaccatttt tcataaatat aatataaata ttttgtttat ttacgtattt tcttagtaac
     6061 ttgtacgtat aaattataga taaataaatt ataaggtagg ggataaattt gagtaacata
     6121 ataacgaatt taatagagtc aatatttact aatccaataa ctatattgtt tttacttgca
     6181 ataactgtgt attctgtttt taaaataata gaaaataaca gagtatattc atttgtaagt
     6241 tcaaaattag acgaaatcaa tagagactat aagacaagtg agttttataa aaaggtaagg
     6301 gatgactact atacatatag taaagaaaat ccttatgcag acgtaaatat aacttcattt
     6361 atagaggaag ttgtatctga tttaaagcat aataatttgc cattgttaga aaaaattaga
     6421 agtataaaaa attcatcttc aataagtata ttacttgggg ttttaggtac gtttgttggt
     6481 ctttctacaa tgcttttatg tgttgatact aaagacatta taaattcact tccttcaact
     6541 attagtagta tgcaaactgc atttacaact agtatatttg gagttgtatt ttctctaatt
     6601 ataggttact ttactaagat aaaagattgt gaacatgttt tgatacaaat aatgttaaaa
     6661 tctgaaaatt tactaacatc tgaaataact cattttaagt cagaaagaat ggatttaaaa
     6721 gtagaagagg taaaaaatac aataaaacaa ataagtaagt ctatagaagc tattgaaaaa
     6781 tttgataaaa tatctaaaga tttaaatgac tttaatgatg aatttatcag tggtatagaa
     6841 gctttaaaaa gccttttaga aggttctcaa agttctatta agacatttga ccagagcgta
     6901 agaaaattag ataaacagtt taatatttta aatataaaat ttgtaaagct ttttgataaa
     6961 tatgataatc aagataatat aaataaagag attttatttg atataaaaga atcttcaaaa
     7021 aatatataca atgccactga aagccaattc aaaattagag attatataaa aaatataaat
     7081 gcaggatttg ctctatatga aagaagtgct caagacttat taactaaatt aatgacccat
     7141 gaaaataaaa tctctcaaaa tcaaaaaatc cttttagatg aaaagtttac cttagatgac
     7201 tcaataaaaa atctctcaag tattattgaa aacttttcta atgacctaca agttaaactg
     7261 gatatgatgt ttgaaaattc tttagatatt caagataagt tagatgttat gtttaataac
     7321 tcttttatga atgatgaagt tcctcttgat agtgaagaat tatttaagga tgatgttaat
     7381 aatgtattta atccttttag tgaagaaatt tatgagatag aggataaaga aataaaggtc
     7441 ataggtgagg atgagttaaa tgaataataa atatagaaga actgttaaca gagaatttga
     7501 aaagagtagc ttttggccaa catttacaga tttattatct actgtattga tggttgtgat
     7561 tcttatctta tttagctcag aaagtatttc aggttctgtt gaacaagatt tagctaagaa
     7621 tgtcaatgct tctgttgaag aaacttttaa aaaaagtgga atacctgtga aggtagataa
     7681 atctaatggg caagtaactt ttggtgaaaa aaccatgttt gatgtagata gtgatgtttt
     7741 aaaaccagag gctaaagaaa tgctaaaaat gtttgttcca aagtacatag agactattta
     7801 taaagattat ggtgactata tatccaaaat agttatagaa ggtcatacag atgatgtggg
     7861 aagttatata tacaacttag atttatcgca aagaagagca tatagcgttg ctaaatttat
     7921 agtgggagat gaaataggtg actataaata caaagataaa gtgactaagc atattattgc
     7981 aataggaagg tcaaaagctg aactcatcaa aaatggtgac aatagcgtga atagagatgc
     8041 atctagaaga gtagaactaa aatacgaaat aaatattaat caaaataaat aattaaattt
     8101 ttataaaaaa aaagaaggca gttggtgtga gctttaagcc ttctttaagg aaaaagttta
     8161 tttatttttg ttgatactta acatattata gtttttagaa gcagtttgca atatattaaa
     8221 cgtaaatggt cgtttaaaat gcataactgg atactactga tgtaaaatat ttcattttat
     8281 ataatataaa aattatacag tgtcaatttt ctatattatt gtactataat ctattattta
     8341 aaatattatg taaaaaaata tcataaaaac gcatatcatg ttataattac tagagtatta
     8401 tgaatatgga ggcgtagttt gtgatagaaa taagtataaa aaataaaaaa aattctaaaa
     8461 ttaaaacttt gatactttta ttgatagtat ctatatcgat ttatttaact ggttgtacta
     8521 catcagataa tggaaataat caatcaaata ataatgattc tttgtctagt agtatatcaa
     8581 gtgataagaa tgtaaaaatt cattttattg acactggaaa tagtgatgca atattgattc
     8641 aagatggtaa aacttttaca ttgatagatg ggggagataa tgatgatgag ggattaatgg
     8701 tagattacct aaacaatcaa ggtgtaaaag atattaaata tcttatagct acccattctc
     8761 atgcagacca tttaggtgga cttgattctg ttgtcaaaaa ctttaatata gaaaatgttt
     8821 ttgtatcgaa tggttcagca gaaacaaaga gttataggga ctttataaat gctttagcaa
     8881 ataagggttt atccccaagt gtaccgcttg aaaataataa gttctattta gaagattctt
     8941 attttgaagt tctaaataca aatgggggag atactactaa tgaacaatct ctagtccttg
     9001 tatatactaa tgggaatgat aaagttttat ttacaggtga tgctgaagaa ggtaccgaaa
     9061 aagaaatact acctaagctt gagaaagtag accttttaaa agtagctcat catggttctc
     9121 gtagtagttc atctcaagag tttttagata aagtaaatcc tgaatatgcg gttcttttag
     9181 taggcaaggg taattcatat ggtcatccac atcaggagac aatgaataaa ttaaaaaaaa
     9241 tgggagtaaa agtacataga agtgatgagt gttcagatat aatatttgaa tcaacaggtg
     9301 atggagtatt tacaagttgt aaagatggaa gttataataa aggcgttaga gaagatggaa
     9361 actctggaaa atcaaacagt aatacaagta aaaatgattc ttttaaaaat aaacaaaata
     9421 catccaattc agaagaaata gtgtatttta ctccaaaagg taagagttat cactctacaa
     9481 aaaattgtag tgggctttca agaagtaaaa aaatattatt aggcacaatt gcagaaagta
     9541 agaaaaatga cccatgtgat atatgttatg gaaaataaca aaaaaacaac caatttatct
     9601 ataaagcttc tgaactttaa atcatttaaa aaataaaata aatgcataaa aaaataagct
     9661 ataatcaata gataacacat tttatctata cgaatagctt atttttttaa gtatttttta
     9721 atagctattt aataactcta aatacaccag ttacaatacc tacaatttca acttctgagt
     9781 cattgagcat aattggttcc ataaaatcat tttctggctg aagtcttatt aaatttcctt
     9841 ctttaaaaaa cctcttaact gtagcagatt caccatttat aagtgcaact actatctgag
     9901 aatttaaggc tgtatttttt ttgtctacta caacgtaatc ttcatctaaa attcctgcat
     9961 taatcatact ttcgcctttt actcttaata caaaattgtt actgcccttt actaaactgg
    10021 cagggaatgg aatgtattcc tctatgtttt gttgagctaa aatgggttct ccagctgtta
    10081 tttgacctac taaaggaagc tctattattt cttgattagc tccagaaaca tcatttactt
    10141 tactacgttc taaaacttca atagctcttg gtttagtagg gtcctttctt atgtatccca
    10201 gtttttcaag tttatttaga tgagagtgca cagttgaagt agatcttaac cctacagcag
    10261 tacatatttc tcttacagca ggtggataac cttttaatat gatttgagac tttataaatt
    10321 ccaatatcaa gacttgcttt tcagttagat ctaaatacat aacatcactc atttccaaac
    10381 atattttgtt tttatttcca tacactttat atttttaatt ataacataat ccatttatat
    10441 ctgcaaacat ttgttcctga tttttaatcg tcctcaatcc attcaaaatt cttgactata
    10501 tcacgtttta catttttctt atgagctgca taaagctgag tagttgtgac attatcatgg
    10561 tcaagtaagt tttgaacatc atatatagaa aatcctgttt ggattagaga agttgcagcc
    10621 gttgctctta atttatgggg actatatcca ttgtctctag tagtatcaag tggaatagat
    10681 gtatattttt ttaccagtgt tcttatagcc tttgctgtaa ttctcttatt ttgaagagac
    10741 aaaaataaag catctttttc ttcatcattt aatagttcat ctcttgttct ttcgttttgc
    10801 aaataatctt ttattacatg ttcacaagta tgatttatag gcattaaaac ttctttccca
    10861 cgttttctat atattttaaa ttcacctctt gaaaaattaa aagaggagat attgagttct
    10921 ctaagctcat ttaatctaag cccgtatgta acaaataatg ctagtatagc tttgtcacgt
    10981 aatttagttt ttctccaata taccttttct ttctctgtaa gcccttcacc tgtttctact
    11041 gattcaagca tcttagcaac ttcatctatc tcaaggcgct taatagcatc tggctgaggt
    11101 ttaggaagtt ttattggatt aaatccatca gttatattat tatctatctg agagtttctg
    11161 tataagaatt taaatagagt agagatggat gatttttttc ttgctaaagc tctattgtta
    11221 ttttcaaaaa tcaatgtgtt tttttctgta tttttgtaat atctactgca ataatcacct
    11281 aagaataaat ttatatctct tgattttatt gtattaaaat catcttctgt aatatctttt
    11341 acacaatctg catttgttaa ttctttggtt tcaatcaaat atgaacaaaa aaaattaata
    11401 tcttctaaat aagcaagtcg tgtagaaact gcaacagaac cttttaaata tataaaataa
    11461 tccttcataa atacaggaag tttattttcc aaaagtagac atttttctat gagttcattt
    11521 tctttgataa caatattatc aggtttatct gatttattat gtacttttat aattttctta
    11581 tcattattca aaattaaacc tcctaaaact aaaatataaa tcttttatat tattttttga
    11641 tatttatacc aagtgagtat aaatatttaa tttaatcatt ttttacattt ttaagagatt
    11701 agcagtgctt tagatgaaat tatcactaac tattccctaa atccacattt aaagactagt
    11761 tgaatttaag tactctt
//
LOCUS       DACXMZ010000115        11731 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.116, whole genome shotgun sequence.
ACCESSION   DACXMZ010000115 DACXMZ010000000
VERSION     DACXMZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.116"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..2032)
                     /locus_tag="JD016_11965"
     CDS             complement(<1..2032)
                     /locus_tag="JD016_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAU5000349.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSINEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITII"
     gene            complement(2055..3311)
                     /locus_tag="JD016_11970"
     CDS             complement(2055..3311)
                     /locus_tag="JD016_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAU5000350.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            complement(3360..5024)
                     /locus_tag="JD016_11975"
     CDS             complement(3360..5024)
                     /locus_tag="JD016_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HAU5000351.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGDGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLSIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            complement(5014..5862)
                     /locus_tag="JD016_11980"
     CDS             complement(5014..5862)
                     /locus_tag="JD016_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAU5000352.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            complement(6092..7144)
                     /locus_tag="JD016_11985"
     CDS             complement(6092..7144)
                     /locus_tag="JD016_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HAU5000353.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPIDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            complement(7144..7872)
                     /locus_tag="JD016_11990"
     CDS             complement(7144..7872)
                     /locus_tag="JD016_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000354.1"
                     /translation="MYAPIFALVLFSFNDSKSMARWNGFTWKWYGQLLQNESIMSALY
                     YTIVIAILASVISTIVGTISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLF
                     VFVKMEFGFTTMLLAHIMFCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVI
                     LPQIKPGIVSGFLIAFTMSIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALST
                     IMFAVVLGLLLLANKKESIVRGIK"
     gene            complement(7923..8759)
                     /locus_tag="JD016_11995"
     CDS             complement(7923..8759)
                     /locus_tag="JD016_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000355.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            complement(8761..9804)
                     /locus_tag="JD016_12000"
     CDS             complement(8761..9804)
                     /locus_tag="JD016_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000356.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            complement(9823..10362)
                     /locus_tag="JD016_12005"
     CDS             complement(9823..10362)
                     /locus_tag="JD016_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAU5000357.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            complement(10798..11487)
                     /locus_tag="JD016_12010"
     CDS             complement(10798..11487)
                     /locus_tag="JD016_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000358.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
BASE COUNT         3914 a         1986 c         1117 g         4714 t
ORIGIN      
        1 caattatagt aataaaagaa atcattaaaa atactttagt tgtcttattt atactcatat
       61 tccaacttga atcttttact tctttcttag attttaaatt ataagctaaa tcttctacct
      121 ctccgtattc atcaattatc aatgtatctc cttttaaatc attaacttta cttttattca
      181 acaaataatc cattcctaaa tataagtttt tttcatttat tgaactaatt accatgatat
      241 ttttttgatt attatatggt gattttatta attgaatggt agaaatattt tttccataat
      301 catcaataaa gcttatctta tcatttgata taaaattgga gtaattttta tcgaacttta
      361 tatttaaatt attatttaac atctttataa cagaattatt atgtggcact ccaaaaacta
      421 caatattagt atccttatac ttgtcactaa attcttttcc tctaattaca tttatatttc
      481 cattatgtga atttatattc gcacctaaag taacacctaa tctaaacatc caggtcatag
      541 cttgtgaccc tgaataatct ggcattatca cagttaaatc attaaattca tcatctctaa
      601 caaatggata cggataattt tcaaaagata aggtttcatt ttctttcgta gataacgcca
      661 aataagaatt atttgataca tatgcccaag gattattact ctctctagta acacaattcg
      721 aattttttat actcaaatta aatgtcaatt taacttgata atagttttta ttatctatat
      781 ctttaggtat ttttaattct aacttatcat tattagaatg tgacctatcc aattttttac
      841 ttcccacaat aacatcattt atagacactg taactaaaga tttttcaaaa tctaaattgt
      901 cagaatatct taaatttaag ataatctttg aaccatcatc taatacctta cccgttggta
      961 ttttgacatc aaacaaagct tgttgattaa atgctccttc caacaaaaag tcagaatatc
     1021 ctaagtcttt taatgttaaa tgacctaaat ttaatttttg ttccctatta atttttatat
     1081 tagtctcttt gtttacaagt actgatgatg ataaaacttg gttagaaagt ctattttcta
     1141 ttaataaatt tgatgccttt attaaatcat cttcattact accaattacc accatcatct
     1201 tcttattctt attgtatggt gaatctactt gcttgattat acagtttgaa gataataggg
     1261 tttgttcctt tatacttaaa atatccaaaa tttcttctgc tgtattttca ggtttaccaa
     1321 tatatataat attatcatct gaccaatttt tcatttctga atataattta acatctaact
     1381 ttaaatcatc attttttgtt atttttccaa atgctgatgc caaattaaac actgctgtag
     1441 tctctcctct agtcatatta tctggtacta ctatagttgt atctaattta tgattatttt
     1501 caatttctgc atatggatac ggatattcat ttattgagtt ttccaccttt ttctgcttat
     1561 atctaattga tatatatgat tctttgtgaa taaccatcca gttcccagta ttggaatcat
     1621 cttggcaaat tttatcagat attgttttgt atgtttttat ttttatttca ttatatcctt
     1681 gaattatata atcttttgga actaatactt ctaatgtatt tttataattt gtcttagcat
     1741 ttaatttaat agattttatt ggtacattat ttatcaatac agttaaactt gaaacatctc
     1801 catttaatat ttggctctta ctaaaattaa gatgcaataa aacttcttca atatcccaat
     1861 ttttatttac ttcaaaaaaa gtactattac ttcctataac accatcaata gttatatctc
     1921 tttcaaactt ataattttta actttagtct catcagcatt tactttgtat ataagtgata
     1981 tatttgaaaa aaacaatact aaagatataa ttgatataat aaattttttc atgttaattc
     2041 ctcctgtaca tgaatcaaaa tctttctgtt ttataccatt taacctctct tttaaaaatt
     2101 atatctttta gatactctat cattcctttt atagctacaa acagccatag ttgagagtat
     2161 gtaaaataca ttattgcaac tatgaatata ttttcaattg ttgcttcacc tttttctata
     2221 gtaaggctta tactcacttc tataataaat aaaagataag atagtatcca tattagaaaa
     2281 aagttaattg gaatagaaat ttctactagt ttagatatac ttaaaacaaa taaaatatcg
     2341 gatataatta cagatgttaa aaatagaaaa tatacagaaa aaaagtaagc tatatcaaac
     2401 acaatcttat ttcttccctg tttaaatata ttttttatat atttcattaa tacatatata
     2461 ttcccttttg cccatctagt acgttgcttt atccacacct taacagtttc tggctcttgt
     2521 tcccatgtta tagattgtgg tacaagcttt atcttgtatc ccaatttata tatcctaaaa
     2581 cttatttctg tatcttctgc aattgcttta ctatcccatc ctccaatttc ttctatgata
     2641 cttctcctca atataaagtt agttcctggt attgtacata gattaaaaag ttgccatcta
     2701 ccagcttgtg acatccattg aaagcttaat gtttctatgt ttataaattt ggttaatagg
     2761 tttttatttt tatttcgagt cctaaatttc ccaataacag cacctaattc atcattcatt
     2821 actatagttt gtactagata tctaagtgca tttttatctg gtgtgttatc tgcatcataa
     2881 acagctataa aatctccttt tgaaatcgta tatccaatat ttaatgcatt agactttcct
     2941 tttccacctg ttaaactatc tgtatttatt atagtgaaat tataattgtt gtacctatcc
     3001 tttatatttt ctaaaatttc tttactatta tcagaagaat tgtcatttat aacaataagt
     3061 tccatcttac tcttaggata attaagtaat agcaaagact ccacagttct acctatcact
     3121 ttagcttcat tatgagcagg aacaagtatt gatatcatag gatattcatc aatttctttt
     3181 atttctttat caaagttttt aaaataaaat atatatccac ccatagctaa aataatattt
     3241 ataagcaata aagaccatat actaaacaga ctaaatacga acaaatactc tgatacattt
     3301 aaatctctca taaatttctc ctaaaatatt ttaaaatatt gattctaata taaaatcatt
     3361 tattttaaaa atttcttttt atctattctc ttaaaatata taaatataat cacaaatatt
     3421 aaaacactta tagatactat gaatattaaa attttactaa tattggatat ggatttaaat
     3481 ctagtatcac ttttagttag ttcatctaag tccttatcat agttgactat aatttcatct
     3541 ccattatttc ttatttgtat ttcatcaact tttacccaat tattaaaatc tcttaaattc
     3601 ataaattcta tattctgttt ttctaaatat tctattgtgt tttttagata ctctatactt
     3661 aaatatgaat gaaagaagaa ccctccacta aaacctctta caatggatag tttatctaaa
     3721 ttttctttta tattttgaaa agtaaactta tcttctgggt ctatatatcc taaattttcg
     3781 ggtataaata tattaaactc ttctgtatcc ctaataatat aaggatatgt atttgtgcta
     3841 aattttttat cattattttg atgttgcccc atataagtag aaaaatattt ttttaattct
     3901 ttatatccct ctgattccat tgcataatgt ggcgcttcaa atgcaagagg ataaattcca
     3961 ttctctatac atactctaag accattcaaa actctatctt tgacaaatat cttcatattt
     4021 tcttttaagg gttcatcttt tttaccatcc caaaattcat acccttctcc agaaacttct
     4081 tctttcttgt attgatgagt atatccatgc aatatcacag ttccaccttt atcttgcata
     4141 tattttattg ctttgacaat ttcagggctt tctgataaag ttattacttt atgattttta
     4201 ggatttacat atgctggtat taatgcaatc gtaaatggta tattcttact acttaaataa
     4261 tccgctatct ctacaagatt ttttggttct ctaaaagcat gtacatcttc tattcttaca
     4321 aatatacgtc ctttatcaaa cgtcttaata ttaaaaatat cattaagtga atcgcaaaat
     4381 atataaaata aaactccatc taaatctaat tttgatacat aaaaaagatt tttatcattt
     4441 attatatatg gatacttatt taaggtatca ttaatacttc ctattacttt attacttgta
     4501 tcttttgatt ctacaatgtt aaaaacataa tgttcatcca agttatatga tttacctcta
     4561 taatttacac taactatatt gtttgttttt cctacatagt ctaaattgta ttttttatgt
     4621 tccaaaagat tttctatacc atctcctaac caacatatag ttcctttata tgaatttaag
     4681 gaactaatca agtttttagt gtttttatca ttattatatg agttttcatt gattcctaaa
     4741 ataaatatat gactgtaata atttttattt atctcaccat catagttact caattttaat
     4801 agctctatat ctgaactaaa tcttcccaat agagtcctca taatatttaa aatatctcta
     4861 ttataagctg tttctttctt agaatcatat atgattaata ccttatctgt gctggaagta
     4921 atttctgatg catatgaaga taagctaaaa tttgatattg tcagtactat tgataataca
     4981 actactaatt ttaatagact tttattaaat attttaaaca tcatattgaa gttcctcttc
     5041 aactatattc ttaaaattta tactatctcc aaaactactc ttatattgta aaaatgcaat
     5101 tttgacatct atatttacat attttcccct attgtttaaa tctaagttta attcttttat
     5161 ccttttcttt attctgtctt taacaacttt tgaaccttcc aaagatgtat ttggcattag
     5221 tatccctatc atatcttttt gtaaactata aattatatct tcatttcttg tacattctat
     5281 aatatttgaa ccaatatatt ttactatttt attagttttg ttttcaccaa atatagtctg
     5341 taggtttccg tagtatggta attttactat cataagagaa aaatcagtat tatgtctaat
     5401 agatttactt atctccatat ttacatcatt ataaaaaatc tttaaatttc taagtccagt
     5461 ttcactatca atagtcacta attccttata ctgttcagat agttttttat ttgtattttg
     5521 caattcattt atgcttttgt tcagattacc cattataatt gaagatactg gtgttgctat
     5581 tatccacata tatgatataa attcaacatc catattgtga gtaatattgt tatataaaat
     5641 ataggatgta tacataaata taattattga acttgttatc aaccccacta ctgaatttga
     5701 taaataagtt aacattatta taaaaaaaat tattgctaac attatgaaca cattcaattt
     5761 attttctgtc ctaatcgata gtattactat tccaacaatt aaaaatataa aaacgagcaa
     5821 agccaatatt aataaatcta tttttttatt tatcctattc attttttata aatttacacc
     5881 tccaattatt ctattaagtt gcccattttt tctaattatt attaaagtca ttatacaaat
     5941 aagttttcat ttattaataa actatttaaa ttgcatatga atataactta aacaaaaaat
     6001 aaaactgttc aataattaga aaataaattt tctaaaaaag aacagtttta ttaagtattt
     6061 attaagtaaa tttacttaaa acaacaagac cctattttga ttttaactct atccaagcct
     6121 tgtcatataa ttttatattt tcaccaatat cttcaaaagt ttcacattta tcaagtactt
     6181 ctttagatgg atatgcaaca ggattctgag ttatttctgg gtctaataat tttagtgctc
     6241 ctgtattagg tgttgaatac ccaatgtact cagcatttac ttttgcattc tctgggtcta
     6301 ataaaaagtt tataaacagc tcagcttctt tcttattttg tgctgttgta ggtacacaca
     6361 tagcatctac ccatttatta gttccctctt taggtatagc ataatcaagg tcaggatttt
     6421 gctccattag tgtaactgca tctccagaat acagcatacc cattgccact tctccaccaa
     6481 gcaatctatc cttaccttca tcatttacat aagccaatac taaatccttt tgtttcatta
     6541 aaagattttt agcttcattt atttcttttg gatttacact attcatgcta tatcctaatt
     6601 ttttaagagt tatccctata gtatctctaa cagagtcaaa catcattata tttcccttat
     6661 actttggatt ccaaagtata ttccagctat ctatagggtc tttaacttct ttcttattgt
     6721 acagtattcc aagagttccc cacatatatg caacactata ttcattagtt ttgtcgtata
     6781 caggatttaa aaaactctta tctaaatatt tcatattagg tatattctta aaatctaatt
     6841 tttccaaaag accttcattt ttcattttct ctaccatata gtctgaagga aatactaaat
     6901 catagtgtgt actaccactc tttacttttt gatacatcat ttcattagta tcataagttg
     6961 aatattgaac atctattcct gtttcttttt caaacttatc tataagagac tcatctatat
     7021 aatctccaac attatataca tttaaaactt ttgtagaatc agttgcttta ttacatcctg
     7081 taaataaagt aactgtcatc atacctacag ctataaggga tattgctttt ttaaacttaa
     7141 tcattattta atccccctta ctatagattc ttttttatta gctagtaaaa gtaaacctag
     7201 aaccactgca aacattattg ttgataatgc atttatttca ggttttatac ctcttctagc
     7261 cattccatag atttctatag aaagattgct tacaccattt ccagcattaa agaaacttat
     7321 tataaagtca tctattgaca tagtaaatgc aattaaaaat ccagaaacta tacctggttt
     7381 tatttgtggt aaaattactt ttctaagagc gtatataggt gttgctccca agtccatagc
     7441 tgcatcttct atattatcag gtaattgctt catttttggc aaaactgaaa gtataacgta
     7501 tggcaaacaa aacattatat gtgccaatag cattgttgta aatccaaatt ccatttttac
     7561 aaatacaaac agactcataa gagctactgc tgtaactatc tctgtattta aaataggaag
     7621 atagtttaca tttagtatta atttcttact cttacctctc atcttatgta ttcctatagc
     7681 acttatagtt cctactattg tagatattac agacgctaat atagctatta caatagtata
     7741 atataaagca ctcattatac tctcattttg aagtaattga ccataccact tccatgtaaa
     7801 tccattccat cttgccattg attttgaatc attgaaagaa aatagtacca aagcaaatat
     7861 gggtgcatac aaaaataaga atactaatgc taaatatata ttagaagtta ttttctttaa
     7921 ttttaaaata gtaacccacc gccttctttg tcagattcat ctccaaattt tgacattata
     7981 gacatagata ttaatattac tatcatcata aatatcgata tagctgaacc aaagttccaa
     8041 tctccaacaa ctgtaaactg ttgttcaatc aaatctccaa ccagcattat tttacctcca
     8101 cctaacagtc ttgatattgc aaaagtagtg actgctggca taaataccat agttattcca
     8161 gacattacac ctggcaaact caaaggaaat ataatttttc taaaaactgt catattattt
     8221 gcacccaagt catgagcagc gtttatcaaa tcattatcca tctttgatag tgctgtatat
     8281 attggaagaa ccataaatgg taaaaagtta tacaccatac ctaaaagtat ggcaaaatta
     8341 gtatacaaaa ttgctaatgg ttgtattcca aaccaaccta aaaatgtatt taaaagtcca
     8401 ttttttccta atatagcaac ccatgcatat gttcttaaaa gaaagttcat ccacatagga
     8461 agaataaaca ataatattaa tgaacctctt ctacttacac gagcctttga aattatatat
     8521 gccactggat aaccaactat aaggcatatt aatgtagctc ctcctgctaa aagaatagaa
     8581 cgcccaaaaa ctttcatata tatagggtca attacttctt tataattctc aagtgtaaag
     8641 gcataacctc cacctgattc ttttgtaaaa ctaaaaaata ctactaatat taaaggaatt
     8701 attacaaata tcgcactcca aactacatat ggatatgcta aaaaagattt tcttttcatc
     8761 ttagttattc tcttttctca ttatatggat atcttctgga tatatatcca tgcctagttc
     8821 agtcccaacc tcagcatttt tagtattatg aagtatccac aatcttccat tttcattcaa
     8881 ttctatctcg taatgcactc ctttaaacac tgtagatgta actttcccct ttaacattcc
     8941 ttcttctggc tttaccattt ttatatcttc tggtcttatt actacttcta tattttcgtt
     9001 tttttcgaat cctttatcaa cacactcaaa atctctatta cagaaattaa ctttataatc
     9061 ttctatcata attccatcaa atatgttact ttcacctata aacttagcta caaatgaatt
     9121 tttaggttca ttgtatatat cttgtggtgt tcccatttgt tgaattttgc ccttatacat
     9181 tacaacaata gtatctgaca tagtaagagc ctcttcttgg tcatgagtta caaaaacaaa
     9241 tgttattcct aatttttttt ggattctctt taattctatt tgcatctctt gtcttaactt
     9301 taaatcaagt gctccaagtg gttcatctag taataatacc tttggttcat taactagagc
     9361 tcttgctatt gcaattcttt gttgttgtcc tccactcaat gattcaactt gtcgtctttc
     9421 aaaaccttct agtgcaacaa gtttcaaaac ctcttttact ttagcatcta ttacatcttt
     9481 tggagtcttt ttaattttta atccaaaagc tacattttca taaacatcca tgtgtggaaa
     9541 taaagcatat ttttgaaata ctgtgtttac ttgtctttca tatggtggca aattatttat
     9601 ctccttacct tcaaataaaa ctttaccatc atcagcatac tcaaatcctg ctattatttt
     9661 taatgttgta gtttttccac atccacttgg tccaagtaat gttagaaact catttttctt
     9721 tatatctaaa ttgaaattat ctaaaacagt aagttcttca taatgtttag atatatccct
     9781 taattctatt atattttcta ccaattatta aacacctcta ttctaaaatg atggtggcgt
     9841 acttacccaa atcacttttg ctgtagtttt gccctcgttt gatatatagt gatttgcttt
     9901 cggtctaaag taaaaactct ctcctttttt tactttattt ttcttttcgc ctatatggac
     9961 atatattgaa cctgctaata catatccaaa ttcttcacct tcatgtggct tttcttcttt
    10021 atactgtcct cctggataca atgttattat tattggctcc atttcatttt tttgtgaatt
    10081 tggaattaac cactttaatt tatattttaa atcttcatct tctgtttcaa acatatcttc
    10141 ttttgtaaaa ctgattcttt catcatctat ttcattaaaa aattccctta aatttgtacc
    10201 tagtatttca agtatatcta taagggtagc tatggatggt gatgttaagt tattttctaa
    10261 ttgagaaata aatccctttg acaattcaca tctattagct aactcttcct gggtcagttg
    10321 tttttctgtt cttaatcttt ttatcttttc acctatatcc atgttgcacc tcctagtttc
    10381 tgttttattt tactaaacat tttgtttact aaacattgag tttaaaattt ctaaacaaca
    10441 taggtaatta tatataaaat ttggatataa tacaagagtt tttttgacat ttttttattt
    10501 ttttatttag aataaataca agcattttaa aggctttgga aaatacacag tttaaaggtg
    10561 tttatattaa tatctataaa gtttagtatc tctaattata aattaattca ttcattaaat
    10621 agagaataag acttctagta ctaaaaaaat attgaacaaa taaaaaattg ctagaaatct
    10681 atattttata gtgttctagc aatcctcata ttttaatatt cttttaatat tcaatttatc
    10741 tatctaaaca aaatgttaat ttaaaatact taacatttcg ttgaatttat tcttaaatta
    10801 cttaactata ttttgaacag cagctttttt ctcaccactc atatacactt tgataaattt
    10861 tatatcatct actataaatc tatgtttaat cttactacaa tctaatttaa ataagccaaa
    10921 taccatttgt gtgaaatacc ccaatccaaa tgcacttatt atagttccta taccaacttt
    10981 tccacctaat aaaaatccta ctattaatgc tccaacttct atagtacctc ttattaattt
    11041 aagtgatttt cctgttttct tttgaattgc aaccatcaaa ccatctctag gtccacttcc
    11101 taaaccactt cccatataaa ataccattcc aacagacaaa agaaccaatc ctaatatcaa
    11161 cataattatt cctacaaata aactatcagc ctctggaatt aaattactat ataaaagtaa
    11221 atccataaaa aatccaatta gtagcatgtt aaaaacagtt ccccatccta cattttctcc
    11281 aagaacgaca tcagcaataa ctactataga gcctacaatt atagaagcag ttcctatagt
    11341 tattcctata tgatttgaaa gtccttgatg aaatacatcc cagggagata atccaaggtt
    11401 acaatttatc gtcaatacta taccaactga acatataaat aaaccgacta ctagctttaa
    11461 aaatttccat aatacttttt gcaacaaatt ctacccctcc taaacataat ttaactactc
    11521 actctaaata tcaaaagcca gcagttttct tacaaaaatc ttattttatt aattttataa
    11581 tatacttcta tgatacatta ttttatgcta aataactacc attttattac aacttagtta
    11641 caatttttgt cattattttt tggtgaagta atttatacat ttatccaacc aatttctatc
    11701 cttcaaatgt tttcaatttc atttatatta t
//
LOCUS       DACXMZ010000116        11653 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.117, whole genome shotgun sequence.
ACCESSION   DACXMZ010000116 DACXMZ010000000
VERSION     DACXMZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.117"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            49..2226
                     /locus_tag="JD016_12015"
     CDS             49..2226
                     /locus_tag="JD016_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAU5000359.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KYVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2240..2662
                     /locus_tag="JD016_12020"
     CDS             2240..2662
                     /locus_tag="JD016_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAU5000360.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            2836..3411
                     /locus_tag="JD016_12025"
     CDS             2836..3411
                     /locus_tag="JD016_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAU5000361.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYEDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHIPRFGQKKLIMVYTQAAPISFLFKKYFKYTAKHLKGMGLNNYKTIVVT
                     KAFERNSTKKNFKVLKKAYKLGMKIAIDING"
     gene            complement(3536..4231)
                     /locus_tag="JD016_12030"
     CDS             complement(3536..4231)
                     /locus_tag="JD016_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAU5000362.1"
                     /translation="MKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVDKTYPKDS
                     IIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTRLHKSNVT
                     VTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIELLSRKTT
                     REKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEGLLSFNKN
                     KFEIFYFQNKLDI"
     gene            4417..5142
                     /locus_tag="JD016_12035"
     CDS             4417..5142
                     /locus_tag="JD016_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HAU5000363.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            5202..6794
                     /gene="hcp"
                     /locus_tag="JD016_12040"
                     /gene_synonym="priS"
     CDS             5202..6794
                     /gene="hcp"
                     /locus_tag="JD016_12040"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAU5000364.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMILSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            7147..8070
                     /locus_tag="JD016_12045"
     CDS             7147..8070
                     /locus_tag="JD016_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin"
                     /protein_id="HAU5000365.1"
                     /translation="MWGYMYMKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNN
                     INMFGGTSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKE
                     VLLSIFPEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMAS
                     SAAPVFFPTYNRHVDGGIIATDPSLACIIHAIDSGFRLKNTRLFSIGTGYVYNSIKAD
                     TTEWGAIDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDD
                     CDALEYLMSLGENCDIKDAFGWIKKKSFYRN"
     gene            8188..9147
                     /gene="msrA"
                     /locus_tag="JD016_12050"
     CDS             8188..9147
                     /gene="msrA"
                     /locus_tag="JD016_12050"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAU5000366.1"
                     /translation="MKMIKLATFAGGCFWCMVKPFDKYNGVIKVVSGYTGGHVENPTY
                     QEVCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFY
                     HDEEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYY
                     KGSGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYV
                     DIVSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLG
                     HVFDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            9300..10430
                     /locus_tag="JD016_12055"
     CDS             9300..10430
                     /locus_tag="JD016_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU5000367.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFRINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(10547..11515)
                     /locus_tag="JD016_12060"
     CDS             complement(10547..11515)
                     /locus_tag="JD016_12060"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAU5000368.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
BASE COUNT         4545 a         1321 c         2007 g         3780 t
ORIGIN      
        1 agaaattaca ctatataaaa tataaagtat gatatttgaa taaaaagaat gtataataag
       61 tataataatt tgtatttatt attgggaaag gaaaatgtta atatggatag ttatagacaa
      121 cttaaagcta aaataccctg taaggaaaca ggtatagaaa taaagaggac tttatgtgat
      181 atttgttctc caggagtaca ttgtggaatt gatgcatatg ttaaagatgg taaagtaata
      241 aaaatagaag gtacaaaaga acatccaatg aataaagggt tgttatgtac caaaggtctt
      301 tgcaatagag aatatatcta tagagaagat agaataaaaa cacctctaaa aagaattgga
      361 aaaagaggtg aaggaaaatt taaaccaata tcatgggatg aagcttatag agaaatagca
      421 gaaaaattaa atagagtaaa atctgagtat ggagcaaagc atgtagcttt ttttacaggt
      481 tatggaaagt ggtatagacc ttttttacaa aggtttgcat cttcatttgg ttcacctaat
      541 tatggtacag aatctagttc atgttcaaca tcagcacaga tggctggaaa tgttgctact
      601 ggaagaaact ttacaatgca agatatggag aatgcaaaaa cttatttagg gtggggattt
      661 aatccatact actctaaatt tttaaatagt gtgaatctaa taaaattaaa agaaaaaggt
      721 ttaaaattta taattgtaga ccctagaata acaccgacaa ctcaaaaact agcagattta
      781 catttaagaa ttaaacctgg aactgatgga gctttagctc ttggaatggc gaatctattt
      841 atacaaaagg atttaataga tagagaatac atagaaaagt atgtacatgg atttaatgaa
      901 tataaagaat atgtaaaatt atttgattta gatactgtaa gtagaattac aggagtttct
      961 aaagataata tagtaaaagc tgtcgagatg ttgtcacaag gtccattttg tataaatgaa
     1021 agtgcagctc caatagttca tcatcaaaat ggatttcaaa actatagagc aatgatgtct
     1081 ttatccgcta taacaggtaa ttatgataga ataggtggaa acataccaaa acaagattac
     1141 tttgctggta agataggtgg attccaaaca ctagaaaaag agtttgtaaa agaagttcta
     1201 cctaaaaata taaatgaaag aattggtgga gaaagatttc ctctatggaa tgaaatggtg
     1261 actcaatttc aagctatgga tttacctagg caaatagaag aaaagacacc atatgaaata
     1321 agagctttgt tttgtcatgg gataaatgct agaatgtttc ctaattcagg gagacttttt
     1381 aaagcgttga atagtgtaga tttttttgtg aatatagatt tatttatgac agatacatca
     1441 aaatatgcag atatactttt acctgcatgt acatcatttg agagagaaga atgtaagagt
     1501 tatccaggag ggtatattac atataccaaa aaagttattg ataaattata cgattcaaaa
     1561 tcagatgtag aaatactttc agatttagca aatgctatga atatagatga tgatttatta
     1621 aaagcaggtt atgaagcaag tgtaagacat atgtttaaaa atacatgtgt agatgtagat
     1681 aaacttaaag aaagtgattt accattaaaa tgtaaagatt ttaagccata tatagtaggt
     1741 tcatatacaa gagaagggta tgatactagc acttctaggt ttgaattaaa atctacaatt
     1801 attgaaaaat ataaagattt tgggctggat gcactaccaa catatagagg atttaatgat
     1861 aatatagatg atgaatatac tcatttattg acatcaggag caagaatttc aaatgcacta
     1921 cattcaagac ttcatgacat caaactttca agaatattaa aaaatgaagc tagttgtgat
     1981 atttctagct tagatgcaaa agaattgggt gttatcagtg gagattatat aaaaataact
     2041 actaagatag gaagtttgga tgtaaaagtc aatgttactg atagagtctt atataagaat
     2101 atacatatgt atcatggata tagagaagct gatataaata gtattatttg tgataatttt
     2161 gacccttata gtggattccc tgcgtataga agttgtaagt gtaagattga aaaaatgaat
     2221 agataaatgg agagatataa tgaaaagttt atttaaattt aataaagaat tatgtacagc
     2281 atgtggagca tgtttgcttg catgtataga ccaaaatgat aataatatag acaatggtga
     2341 actttttaga catgtattta caatagagga aggtgaaaat atagaatatt tttcattagc
     2401 atgtatacat tgtaatgagc caaaatgttt aggtgtatgt cctaaaaatt gttttaaaaa
     2461 agaagaagga ttcgttgtat tagacaatca aaactgtata gggtgtaaac tttgtgaaaa
     2521 agcttgtgaa tatggagcct taatttatgg aaatgatggt aaggcaaata agtgtaatgg
     2581 ttgtattgag agatataagt gtggtatgga ttcaccttgt gtaatggtat gtcctacagg
     2641 agcattaaaa tttaataaat aattgtatta aattgctata ctacttttaa ataattgtgt
     2701 taagaattat tttttgctat acttttgtaa tataaaatgt aaataaaaca aatacaaagt
     2761 atataaaata tatttttaga ataaagtgtg ctttagatta aatttagaaa aataaattag
     2821 acaggagaac ttaatatggt aaaagtatta gcctttatgg gcagtccaag aaaaaaaggg
     2881 aatgtagaca caatacttga tgagataata aaaggtgtaa atgataataa tgttgaagtt
     2941 aaaaaatatt atctaaatga tatgaatatt aaaggatgtc aaggctgtct atactgtaga
     3001 aaagttccta cttgtgcatt taaagacgat atgattgaca tatatgaaga tataaaaagt
     3061 gctgaatata taataattgg ttcgccagta tatatatgtc aggtatcagc tcaaacaaag
     3121 ctacttttag atagattgta tcctttaaca gagataaata agtctaaaca tatacctagg
     3181 tttggtcaaa aaaagttaat catggtctat actcaagcag ctcctatttc gtttttattt
     3241 aaaaaatatt ttaagtatac tgcaaaacat ttaaagggca tgggattaaa taactacaaa
     3301 actatagttg taaccaaagc atttgaaaga aattccacta aaaaaaattt taaggtattg
     3361 aaaaaagcct ataaacttgg aatgaaaata gccattgata tcaatggata gataaaagtt
     3421 taatttatag tatagctaaa ggagcattaa ttattaatac tccttttttg tataaaggtt
     3481 tctagaaaat aactaataaa aaacaatatt atttgaatac agatattata caagcctaga
     3541 tatctaactt gttttgaaaa taaaagattt caaatttatt tttgttaaaa gataacaagc
     3601 cttcatctcg catcttgcca agctctcttg acatagaact tctgtcaaca cataaaaaat
     3661 cagccatttc atttcttgaa aagggaattg taaactggtg gctacctgtt tgttctatct
     3721 gttttgaaaa atacataagc aatttctcgc gagttgtttt acgagaaagt aattctattt
     3781 tattgtgaag taaaatgttt ttttcagcta ttaattgtag catattttca acaagcctat
     3841 tatgaaatac acatgcagat gaacaaatac cagttacact tttaaacttt ataaataaaa
     3901 cttcgcaaga agatgttgtg gttactgtaa cattactttt atgtagtcta gtacatgcga
     3961 atacctctcc aaataaatct ccaggcaaga gttcagctac aatattccta ttcccttcga
     4021 tatcttcctt tattatatga gcacttcctt taagcataat tccaatagaa gtaacttcac
     4081 ttccaacaga gaatattata ctatcttttg ggtaagtctt atctacaaca cctaaacatt
     4141 ccaacatttc ttcaagttca tcacttttaa ttccttcaaa tagaggaatt tttttaagta
     4201 ggtctatatg cttttctaaa aattttttca aataatcact tccttgttgc atatgcaacc
     4261 gataatttgt tttcattgta ctatactaag tttataaaag caaggggata taaaaatcct
     4321 actacaagaa atttgaaata aagaaatata ggtattaaaa ataaatatat aagaaatcaa
     4381 tactttaaat taaaaaaatt ttatgaggtg atataaatga tgagaaaaat aattaaaatt
     4441 gatgaggaaa agtgtaatgg ttgtggacta tgtgttgaag cttgtcatga agaagctata
     4501 ggaatggtaa atggaaaagc aaaactttta agagatgact attgtgatgg gctgggagat
     4561 tgtctgccaa cttgtccaac aaatgcgatt tcatttgagt atagagaagc agcagaatat
     4621 gatgaagctg cagtaaaagc aaatatggaa gcaaagaaag cacaaaagaa aactttagca
     4681 tgtggatgtc caggttctca gtctaagtca ataaatagag aggtttcaaa tagtacgtct
     4741 ataagcaatg atatagtaga gataaaaggt tctcaactta atcaatggcc agtacaaata
     4801 aaattagtac caactaatgc atcatattta aagaatgcaa gtcttttaat agcagcagac
     4861 tgtacagcgt atgcatatgg aaattttcat aataaattta tgaaaaataa ggttacatta
     4921 atcggatgcc ctaaacttga tgaagttgac tatgctgaaa aacttacaga gattttaaaa
     4981 gaaaatgata ttaaaaatat agtagtaaca cgtatggaag ttccttgctg tggtggtata
     5041 gtaaatgctg ttaagacagc tctacaaaat agtggaaaaa tgataccatg gcaaatagtt
     5101 actatatctg tagatggaaa aattgtagat ggagaaattt aattaagtga attatagaaa
     5161 aatataataa attttaataa ataaaaaggg aggaataaaa aatggaaaaa atgttttgtt
     5221 ttcaatgtga acaaacagca ggaggtaaag gttgtactat aaatggtgtc tgtggtaaaa
     5281 aggggggtac agctaactta caagacgaac ttacaggagt gttgattggt ctttcaagag
     5341 caactattgg aaataagaat agaccaacaa gtgaaacaga taaaattatg atagaggggc
     5401 tattcactac tataacgaat gtaagttttg atgatgaagc tataaaaaga caaattgaaa
     5461 aaactgaggt agaaaaaagt aagcttgtac ctagatgttc agattgttca acaacttgta
     5521 atagaaatga taactatgat atgaaggatt tatggaatga taatgaggac atacgttcat
     5581 taaaatcatt aatcttattt ggattaagag gtatggctgc ctatgcatat catgcaatgg
     5641 tacttggtta tacagacaaa gacgtaaatg aatttttcta tgaaggatta atagcagttg
     5701 gaggagactt gtctattgac gaacttttag gtctagttat gaaaactgga gaaataaact
     5761 taaaatgtat ggaattgctt gatagagcaa atactgaaac ttatggaaca ccagaaccga
     5821 ctcaggtatc tatgaaaatc gagaaagggc catttattgt agttacagga catgaccttt
     5881 atgatttaca caaattatta gaacaaacga aagataaggg tataaatata tatactcatg
     5941 gtgagttatt accagcacat gcatatccaa aactaaaaga atataaacat ctaaaaggta
     6001 attttggtac agcatggcaa aaccagcaaa aagaatttga taatattcct gctccaatat
     6061 tatttacaac aaactgttta atgcctgtta aagatagtta tagagataga gtatttacta
     6121 cagaagtagt tgcttatcca ggtatggttc atattgatga agataaagat ttttctagtg
     6181 taatagaaaa agctcttgaa cttggaggat atagagaaga tgttcatatg actggtatta
     6241 atggaggaga tacattaact actggttttg caagaggtac agtttcatct gtacaagata
     6301 aagtagtaga tgctgtaaag agtggagcta taagacactt tttattggtt ggaggttgtg
     6361 atggtgcaaa acctggaaga aattattaca ctgaatttgt aaaacaagct cctaaagaca
     6421 ctgttatatt gacacttgca tgtggaaaat atagatttaa tgatttagac cttggggata
     6481 taggtggctt acctcgttta atggatatgg gacaatgtaa tgatgcttat agtgcaatta
     6541 aagtggcagt ttcattggca aatgcttttg aatgtggagt aaatgatttg cctcttagca
     6601 tgatactgtc atggtatgag cagaaagctg tttgtatact tcttacacta cttcatttag
     6661 gtatcaaaaa tatctatata ggaccttctt taccagcatt cttatctaag aatgtattag
     6721 atatattggt tgataaattt gcattgacac ctatatctac tcctgaagaa gatatgaaga
     6781 aaattttagg ataaataaat atatctatac ttaaagatat ttatagttaa actattttta
     6841 agtatgccac aattaagata aggggttaaa cttaaaattt gattaaaaaa tcaattttga
     6901 gatgaccctt tatttatttt ttgtaataat tgaaaaataa acctgctttc agtatatttt
     6961 tttaagaatc tttgtattaa atttataata cttagatata taatatctta tatattttat
     7021 tttaagtatt aaaggtttga atgtaactta aatattaaaa gaggaacatt gtaatatgta
     7081 acttgcataa aatatatata ctatagtgaa attattttga ttaaaattta aaatataagg
     7141 tgttttatgt gggggtatat gtatatgaaa aattctaatt ttaatatatt ggcatttgat
     7201 ggtggtggat taaaaggggc attgagtata tccatacttg agaggatagt aaaagagtat
     7261 ccaaatctat taaacaatat aaatatgttt ggaggtacat ctacagggag tctaattgca
     7321 ctagggcttg cgtatggtgt aagccctaaa gagataaaag aactttactc aattgaaaat
     7381 tcaaagtata tttttaataa aagttatgca gaaatactca gaccaaagta tgaaaacaaa
     7441 aacttaaaag aagttttatt aagtatattt ccagaggaac tagaattaaa agatttaaat
     7501 aaattagtaa tgataccaag tttttatata ggaaatgagg aaaatgcatg gaaaccagta
     7561 ttttataaca atatgccaaa ttcttttaca aagactagca aagttgtaga tgtagcaatg
     7621 gcaagtagtg ctgctccggt attttttcca acatataatc gtcatgttga tggaggaata
     7681 atagcaactg acccgagttt agcgtgtata attcatgcca ttgacagtgg atttagactt
     7741 aagaatacaa gacttttttc aatagggact ggatatgtat ataacagtat aaaggcagat
     7801 actaccgaat ggggagctat tgactggata attaataaag aacccgattt accaattata
     7861 tctattacct tagaaggaaa ttctcaaatg agtcagttat tttctcaaaa acttcttgga
     7921 gataattact atagattaaa tcctaagatg gaaaaagatg tagctatgga tgattgtgat
     7981 gcattagaat atctaatgtc tttaggagaa aattgtgata taaaagatgc ttttggatgg
     8041 ataaagaaaa agagctttta tagaaattag attcaatttg caattgtatt tttacacatg
     8101 atttcattga gtaggtaaaa ataataaaat gcagattatt aagttacttc ttagggaata
     8161 aataaagtat aatttattta aggagtgatg aaaatgataa aacttgctac ttttgcaggt
     8221 ggatgttttt ggtgtatggt aaaacctttt gataagtata atggagttat taaagtagtt
     8281 tctggatata caggtggtca tgttgaaaac cctacatatc aagaggtatg ttcaggaact
     8341 actggtcatt tagaagcaat tcaaataaca tatgatgatg atataataag ttatgatgaa
     8401 cttctaaata tcttctggaa acagattaat cctacagatg atggtggaca atttggtgat
     8461 aggggaactc aatataagac tgctatattt tatcatgatg aagaacaaaa aaatatagct
     8521 ttagagtcaa gagaacatct ggaaaatagt aaaatatttg atagtaaaat agtgactgat
     8581 ataattccag caactaaatt ctacgaagca gaagaaaatc atcaggatta ttataagaaa
     8641 agaccattaa actacaatat gtattataaa gggtccggaa gatataattt tgtaaaaagt
     8701 cattgggatg gaaacaatat aaatagggaa gagttaaaaa agactttaac tcctatacaa
     8761 tttgaagtta ctcaaaatga tatgacagaa gtaccttttg aaaatgaata ttatgacaat
     8821 aacaaagagg gaatttatgt tgatattgta agtggagaag tgttattttc ttcaaaagat
     8881 aaatttgatt ctggatgtgg gtggccaagt ttttcaaagc cagttataga taaatctatc
     8941 attgagagaa gtgatttctc acaaggtatg tacagagttg aagtaagaag tgctaaagga
     9001 aattctcatt taggtcatgt atttgatgat ggtccaaaag aacttggagg tttaagatat
     9061 tgtataaatt ctgcttctct taaatttata ccaagagaaa atatggaagc tttaggatat
     9121 ggagaatatt taaaactttt tgagtaatta ggaatattta ttataaactt atctaaaagg
     9181 tgaaaatcta aaaaaatatt ttcacctttt gtttttttgt caatagtatg ataaattatt
     9241 aattgtaatc ttatttatac caatagatta agaatttaat tttgaaagag agaaaacaaa
     9301 tgaatttaaa agactttaat ggaaagagat taaaatcagc tagaattttt agagcaaaga
     9361 cgatagaaca gttgtctaaa gaaactaaaa taaataaaaa agatttaaaa gcttttgagg
     9421 aaaataagta tgtaccaaat atagaaaata cattaaaatt atctaatata ttaaattttc
     9481 caaaagagta tttttataaa aatgaaaata taaatatcgt tgtagaggat agccacttta
     9541 accctcaatc taaactacct agagtagaag agatttctta tagagaaaaa ataattataa
     9601 tacataaaat atattcattt atggagaatt atattaagtt tccaaaagaa aacttgccaa
     9661 atagaaaagt aatgtcagat attaatataa atgatataga atctcttgca tataagacta
     9721 gagagttttg ggatttaaat agtactccaa tagtaaatat ggtaagtttg ttagagtcta
     9781 aagggattat aatatcaggt atgaatgtag atagaaaagg ggcaactatc tttactcaaa
     9841 aacagagaat tagtaaagaa tctaaatatc taatatctct gggtaatgac aaaaagtcag
     9901 catcaattag aaattatact ttggcatgtg aacttggata tataatatca agtgaattaa
     9961 gaataccaag taagcaattt tcagaagatg agtatgcctg tgcattttta ttgccaaaag
    10021 agagtttttt aaaagatttg actcatccag aagatttaga ttattatgta gaactaaaga
    10081 aaaaatggat tgtacctata tctgctatga tttttagagc atataatcta gaaaagataa
    10141 attacaaaaa atacaattat cttatgaatg aaatggataa aaaaggctgg ttgatagaag
    10201 aaccactaga caaaatgaaa ggttcaagcc caacgtattt aaaaagagca gttgaattat
    10261 tgatagaaaa taaaataatg agtgtaaata gtatagtatc aagtttagag gaatttcgaa
    10321 taaatcttta tccagaagac ttagaattat tgatgggatt gaaaaaagga ttgttatctc
    10381 aagaggtaaa taaaaaaagt aaagttataa aatttgatgg taaaaaatag gtttatattt
    10441 tagtattaaa attaaaagca ggtacaaatg atttaaacat taacttagtt aaaagtcttt
    10501 tcaataagtc atttacctgc ttttagtgta agaaagaata ttgaatctaa agtgtttttg
    10561 aaagtgcaat atattccctt aaaacagaac aagatttatt aaattcgttt tgctcatatt
    10621 cagtcatatt gatttcaata atttcttcaa taccatcttt tcctataaca catggaacac
    10681 ctgcaaatac atttctttcg ttatattgac cttctagtaa tgtagaaact ggaagaactt
    10741 ttttctcatt gtgtagtata gcttttacaa tttctactaa agcagtacct ataccaaatt
    10801 ctgtacaatc tttaccattt attatactta ttcctgtttt tttatttttt tcaactattg
    10861 atggtaagtc taaattgcta taagtacttg ggttgtcttt tatcatgtct aagattggtt
    10921 taccaccaat tgaaacatga gaccaaggaa ccatttgaga atctccatgt tcacccatag
    10981 aataagcttg aattgattgt tgagcgattc cagtttcttc agataaaatc cttctaagtc
    11041 ttgttgaatc taaagctgtt ccagttccaa tgactttgtt tttaggaaag ccagattttt
    11101 cccaaatata atttgtaact acatcaacag gattagatat aactacaaat ataccatcaa
    11161 agccagaatc aactatgggt tttactatag attttatcat tccaatagtt ggtcttagta
    11221 aatctaatct agttgtgttt tgtctacgta aaggcccttc tgaagagtct gcaacactta
    11281 tcactactat gtcagaatct ttacagtcag aaaaagttcc tttttctata tgtactttat
    11341 gtggcagata tgaaactgca tcagctaaat ctaaagcttg agcttttgat ttagattcat
    11401 ctatatcaat cataaagagt tcatcacaaa ctccttgagt tattaaacta aatccacaat
    11461 gagaaccaac atgccctgaa cctacaatag atatttttct tggctttatt gccattttaa
    11521 atcactcccc ctaaaataac gatacattga aaattataga tgttattaaa agctgttttt
    11581 ggatatttta atattctatt atttcagaaa atttatttaa tatgatttta tactaaaatt
    11641 tttaaaaaag caa
//
LOCUS       DACXMZ010000117        11630 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.118, whole genome shotgun sequence.
ACCESSION   DACXMZ010000117 DACXMZ010000000
VERSION     DACXMZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.118"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            279..1457
                     /locus_tag="JD016_12065"
     CDS             279..1457
                     /locus_tag="JD016_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAU5000369.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNKISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            1459..2688
                     /locus_tag="JD016_12070"
     CDS             1459..2688
                     /locus_tag="JD016_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAU5000370.1"
                     /translation="MNIYMSLLVILLCLLSYSIYHYNFFKYLDIMINNSKNKYKVYIV
                     YFLFNYLIFIICTYLRLHLVINWTIFMLILFMQIKVTYKLSTIKNLFYSLNCSIIGLA
                     INIFFRSLMSIVLNMPAYLFDSNSITNIYRVFSILLSFIVTGLYFSYKNHKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            2897..3046
                     /locus_tag="JD016_12075"
     CDS             2897..3046
                     /locus_tag="JD016_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009302181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAU5000371.1"
                     /translation="MTTVAEGIETSFQKDFLQEINCDMLQGYVFSRPLPIKDFEKLMF
                     QNSSN"
     gene            complement(3149..3313)
                     /locus_tag="JD016_12080"
     CDS             complement(3149..3313)
                     /locus_tag="JD016_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000372.1"
                     /translation="MSKKPIFALKLSTFVIALAEVVILKVFRFHNTTNTHRTAVRKTV
                     AWWVFSYAKK"
     gene            complement(3306..5873)
                     /locus_tag="JD016_12085"
     CDS             complement(3306..5873)
                     /locus_tag="JD016_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HAU5000373.1"
                     /translation="MIYYVLPLKHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQSFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGLSEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFYE"
     gene            complement(5876..6481)
                     /locus_tag="JD016_12090"
     CDS             complement(5876..6481)
                     /locus_tag="JD016_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAU5000374.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSLEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6855..8315)
                     /locus_tag="JD016_12095"
     CDS             complement(6855..8315)
                     /locus_tag="JD016_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HAU5000375.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8639..9226
                     /locus_tag="JD016_12100"
     CDS             8639..9226
                     /locus_tag="JD016_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAU5000376.1"
                     /translation="MIKMAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSK
                     SGIAKGSFYTFFDSKEKLFFEVLNEIQNKLISSVDEILSNMPPKEGFAKSLKMLYRLY
                     DKNPFLYLPNNHDYISLINKLPQDMFSQLEYNNIQSFYDLIDKYNLKFKIEKEKAFGI
                     CVALLSGLLVKENIEYNHIEVFDFMVDNLVEHILE"
     gene            9277..9508
                     /locus_tag="JD016_12105"
                     /pseudo
     CDS             9277..9508
                     /locus_tag="JD016_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9517..10767
                     /locus_tag="JD016_12110"
     CDS             9517..10767
                     /locus_tag="JD016_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAU5000377.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLTSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKYLKVTNLHIPFIICALIFVPMAVSVYPFILNIGFPVPFLIFIVSSMLIFF
                     TTSLTSIYVITITQLKTPNELLGKVMSILFSGTSIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            10928..11497
                     /locus_tag="JD016_12115"
     CDS             10928..11497
                     /locus_tag="JD016_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAU5000378.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         4015 a         1917 c         1641 g         4057 t
ORIGIN      
        1 catagttatc atacatgcta tatgtaatta ctatacttat acacataact attatacttt
       61 ccatacataa ttactatatt tggacacata gttatcatac atactgtata ttaagatata
      121 atacttttta aattttatat aacacagaca catgcaataa aaaagaaggt atccataata
      181 agctctttgc atgtacctaa ataaaaactg taaaatagaa tatataaaat ctaatctatt
      241 ttaagtaaat acaatttcac tttaaaagag gatgtactat gaaataccat atatttttaa
      301 tcataactac tatattaata atcgaactat gtaacattta catatgcaaa atagcatctt
      361 ttggaaacaa aaatataact atacataaga attacaaata tattttttta ataaaaccat
      421 taaccttctt gataataata ttcacaaaat taccattacc actatattat atattgtttt
      481 acatagtaag tcataaattg ctcttaagca gaaaaagttc tactaacaac cattttattc
      541 ccaatatggc agtaataagt ttttgtattt tacaccttat tactgtaagt acaatatcac
      601 ttgttaaaaa tacaagtcca gatataatat ttgcaaatat gacctataag ttaattagta
      661 ttttaatagc ttctatttta gattttatct tatggtactc tatgaataaa ataaaagagc
      721 atatttatat aataactcaa aatcaaatag agttcagtct atttttaaaa tttatctggt
      781 tcactttagg atatatatta tttgatagta tacaaattac atttgctcca ccaagttctc
      841 taacttccta ctttatattg ggaagtaaca tattattaat cttacaacta attatattca
      901 tcaatcatat atattccatt gaaaaaaata tttatttaga aaaagaacat aatagactaa
      961 aaatgataaa acaagaacaa ttactaaaag taaatttatt aaagaaaaaa gctaacattg
     1021 atgaattaac atcagcatat aatcgaaaat ttgcttttga aaaaatacag cattacattg
     1081 ataacaattt attattttca ttgatatata tcgatttgga taaattaaag acgataaatg
     1141 atacatatgg acatttagct ggggataact atattattaa cttctcaagt atagttattg
     1201 aaaacttagg aatagatgac atatttgcta gaataggagg agatgagttt atagttatta
     1261 tgcctaaatc taatgaaaac accacaaaag aatttataga gaaaataagt aaaattatgg
     1321 ataataagat atcttttagc tatggtattg tagaagtttc tagcgaaaac aaaatgactg
     1381 tagaagaaat tattaatatt gctgatatta gaatgtacac taataaacaa aataatcata
     1441 aaaggaacaa taactaaaat gaatatttac atgagcttac ttgtaatctt actatgcctt
     1501 ttatcttatt ctatctatca ttataatttt tttaaatatc tagacataat gattaataac
     1561 tctaaaaata aatacaaagt atatattgta tactttcttt ttaattattt aatttttatt
     1621 atttgtactt atttaaggtt acatctagtt ataaattgga caatttttat gttaatttta
     1681 tttatgcaaa ttaaagtaac ttacaaactt tccacaataa aaaacctttt ttattcgtta
     1741 aactgctcca tcattggttt agcaataaat atatttttta ggtctttaat gtcaatagtg
     1801 ttaaatatgc cagcatattt atttgatagt aattctatta caaatattta cagagtattt
     1861 tctatattgc ttagttttat agttacagga ttatatttca gttataaaaa tcataaaaat
     1921 aataagatta actttgaggc agttttagag gacaaaaaaa acttaatttt cttattgagc
     1981 cttatgtttg gaatgtactt gtatctatgt ttgagtttgt caatatacta tactgttgga
     2041 aatgtattga ttttaaaatt atatggaatc aagtctgcca taatagtttt ggtaggtaac
     2101 tatattgcag aaagatatag ctttagaatt actcaaataa aaggttatga tattaaaaat
     2161 caaatagaat atgtagaaag tgtaaagaca gagtatgaaa ttaataaatt gaatgatatt
     2221 gtatatacag atttactgac aggcttctac aatagacctt ttgtgaatca aaaactatca
     2281 gaatggacat caaatcacta caaatttacc ttatgtttta ctgatttaaa cagcttaaaa
     2341 cctgtaaatg ataaatttgg acaccaattt ggtgataaat acatatccac tactgctcaa
     2401 ataataaaaa aacttataaa tgtagaaaat tccttactat ttagatatgg tggagatgaa
     2461 tttttattcc ttttacagaa tacaagttgt gtagaagcag aaaaaataat gattaatata
     2521 aattatgaat tatcaaaatt aagtaatact gacgagtatc cttatgctct ttccataagc
     2581 tatggattag ttgagtggga tggaatatcc gatattgaaa ctttaatatc aaaggcagat
     2641 tctttgatgt atgaaaataa gcttaaaaat aaatctactt taaactagct agttaagata
     2701 ttctacaaaa tatatgttat aagagagtac taagaatgtc aaaatatata gattttgcaa
     2761 atattgaata cagtttacta atgaatgcac tggatgtaag tgttagtaaa cacctattag
     2821 atgaacactt tactgttgtt tgggctaatg accgttatta tgaaatgttc ggctatacct
     2881 agctaaaaag ttaaaaatga ccactgtagc cgagggaata gagaccagtt ttcaaaaaga
     2941 tttcttacaa gaaataaact gtgatatgtt acaaggatac gtgttctcta gacctttacc
     3001 aataaaagac tttgaaaaac ttatgtttca aaactcctcc aattaacaat ataaaattgc
     3061 ttattataag tgatattatt gtatcaacaa ataaaggaat tgtctcaaaa ttgatttttt
     3121 ctaatcaaat ttttattttg agacattctc atttttttgc ataagaaaat acccaccaag
     3181 ctacggtttt tcttaccgct gttcggtggg tattagtagt attatgaaat ctgaaaactt
     3241 taagtattac tacttccgct agcgcaatca caaaggtcga aagcttcaaa gcgaaaatag
     3301 gctttttact cataaaacgt caccgtacct gccaacccgt ccaccgttat catttgtccg
     3361 ctcttaatag ttcgtgtggc aatgtgtgta gccataacgg ctgggatgcc atattcacgt
     3421 gcgacaatgc ttgaatggct aagaggacca ccaatgtccg ttacaatcgc acttgcagat
     3481 gaaaataatg gtgtccatgc tggagttgtg gtaactgcaa ccaaaacact tccaggttga
     3541 aagctttcaa aatctgctgg actattcagt acacaagctg gagccgtcac aacaccagta
     3601 ctggtaccaa tgcccgtcaa cacaattttc ccatctttct gcttctgtgg tgcttgtgat
     3661 atagtttttt tgtttttttc tggcaacttg gatggtgaca cataacccat gtatttttta
     3721 agttcagctt ttcgaacagg tattatcccg ttcatatcag atagagactt gtttttatcc
     3781 aactgctcta taagtacctc caattccgat tttgtgagcc aataaatatc atctaaatgt
     3841 gaaatagctc ctcctcgtat gaatcgttct gagatttcct gaaacataca acgaatcaga
     3901 ggatgtccca ttcccatcaa ataaatagca ttttcacgca taggagaggt ttcctgtgcc
     3961 caatggagca acttaaaaaa tagtttttta cgcaatccac ctatgtgttg taaaatctct
     4021 tcttctgcat gttttctacg cttttcaaac gtggtctggc gtaaaaaagg actttcccct
     4081 tttccttcca caaaggtttt tacggattca aaagttggtg taagtgtttc ctgcggtgtg
     4141 gaatacgcaa aatcaaactc ataagcggta cgaccaaact ctttaaagta cttattgatt
     4201 cgatttttcc attctatcca tacttcctgt gaaactccaa caggcacaac tgaagacatg
     4261 aaatcttctg ctatctttat tgcaggactg ttttgcagat aaaggttgat actgttattt
     4321 tgctttaccc attctgaaag accccacaac tccttttccg attgcagaac aatcgtatca
     4381 aacccaagca ggaaaacgga agtatccgtt ataccagcac gtcgaactgc tccttgaaat
     4441 aactttgtaa ataatgtttc actgatagaa gcagcaggca atgtaagctg aatccttgtg
     4501 aaataaatac atgcggcata aaatattgtc tgaataccct ccataatttg atgggcgttc
     4561 atagtatgca ggggcttttc ttcccattgt ttaatagcat cctcatatcc ttttcgtgcc
     4621 gtttcccagc gtgcaacgct gttcataagt gtcctacgta aagagcgtgg agaaagggat
     4681 ttgatagcaa tcaaaagtgg ctttgattta gcagaaagat aaacatatcc attaataacc
     4741 gtatatgccc cgttttttgg taataatttt ttcgcacttt taccaaacat atctatccat
     4801 aaaagctctg atgcacggtt tacgatttca agaccaagcg tggcgaacaa aggtgtaaca
     4861 ggatttggca agtgttctgc caaacttccc tttgtgtata caacatcctg ttctggcaat
     4921 acccattctg gtggcagaac agtaattgga cgagcctgaa caatgtacaa tttatctttt
     4981 tccagtgccc actctacatc cataggcatt tgataatact tctcaatttt ctttccaagc
     5041 tgtatcagtt gcattacctg attacgagta agagcatatt tttttctcaa cctttcagga
     5101 accatggttt cttctgttcc ttctgaagta cgaaccgtca taatctcttt atttgccact
     5161 tcatacgata taattcgttc ggagtcctta tccactacga ttgtatcggg tgtaactaag
     5221 ctagaaacca ccgcttcacc aagcccccat gcagcgttaa taatcatttc gctacgtcta
     5281 ccattgattg ggtttaacgt aaacataata ccagatgaat cggaaaatgc aagcttttgt
     5341 actacaacag caagtgcgac aatctcgtgc tttatattat tcttcacacg ataagcaata
     5401 gcacgagctg tccataagga agcccagcat ctctttacag cgtcaataac tttatcttca
     5461 ccttgtatgt taagataagt ttcctgctga cctgcaaaag aagcgtcagg taaatcttca
     5521 gcagtcgcag acgaacggac agccaccgat atgttcccta atccagcata agccatttta
     5581 attgcgtccg acacttcctg tggcatttct ccattatgga aaagctcccc tattttctta
     5641 gatacatctt caagttggct ggtattattg gaatcaatac catctagcag tttattaatt
     5701 cgagactgaa tatggttcgt ctcaacaaaa ctctggtatg aagcagtcgt aacatgaaaa
     5761 ccgtctggca cagggatgcc tgctgtcaat agttttgata aagacatccc ttttccacca
     5821 acaatttcaa tggttgcttg tttatgtttc agtggcaaga cataataaat cataatcatt
     5881 cacctccatc attcataatt ccgtgcagaa atatattcat tgtttcatcc aacatttttt
     5941 ccaaactatc cgatggtcgt gacgtgtagc tgattgataa catggagtta attaacaaac
     6001 gtgtcacaag agcggtatcc gttttgcgaa agatgcctgt ggtcacacca ttatcaatta
     6061 ccgattttat aatatcttga tatgcataac gagcatcttt aagtctcttt cgatactctt
     6121 cttctaaata atcagattct gtatttaacc acatcagtac agttctatac tgctctggaa
     6181 atctaagatg attaagcatg atttttctaa ggcactgttg tggtgaaggt tcaccatcaa
     6241 taatcctatg aacttgttgg atagtttttt ctatttcttt ctcaactgca tatacaacaa
     6301 tctcgtcttt cgttttgaaa aaatcgtata gacttgactt tcccatgttg gctaaaactg
     6361 caatttctct cattgatgtt ttttggaatc cattttcagc catgagactc aaagcaacag
     6421 caactatttt ttctcggcgt tctgcttgtt gttcgggtgt aagcacaatt ccttttggca
     6481 tagggcgata cctcctttta gcgaccactg gtcgtttctt ttttatttat catacagcga
     6541 ccaacggtca cttgtcaagg ttaatttttt tcattgtata tggtactcga gataaaaatg
     6601 ttcattttag aaattgctct atttaagtag aaataggctt catcattaga aaacaagact
     6661 ataaatttga gatatttatc atgttcaaat ttcatcaata tcaaagttta agatattaat
     6721 aaaaaaagaa ttactaagaa ataacatata aagattcaaa aaaggagttg aacttaaaat
     6781 tcaattacaa aaaatcaaat ttaagacaac tcctttattc tttatctcta ctattcttta
     6841 tctctattta tttattacaa ctccctaaca tttaccacta ctgccttagg tctagtcata
     6901 gcctcgattg aatacttaac accttgagtt cccataccag atgcctttac acctaaaaat
     6961 gggaagtggt ctggacctct ttcagtctta ttatttattt gaacagtacc aacttcaagt
     7021 ttatcagcta tataaaatgc cttatcaata tcacttgtaa atactgatga ttgaagccca
     7081 tactctgatt gattagcaat ttcaattgct tgatttatat ctttaactct tataattgga
     7141 agtactggac caaatggttc ttcccaagca attttcatat caacatttac attatctaac
     7201 aatgttggat agaataaatt attttttcta acattgcctg ttattaactt tgctccttta
     7261 tgtaatgcat catcaaccaa cccttgaaca aaatcagttg ctttattatc tataagagga
     7321 gtgataacta cttcatccat tggatttcca attgtcaact tctctaccaa tggctttatt
     7381 tttccgacta atttatccgc cacactctct tgtactaaaa ttctctttac tgccgtacat
     7441 ctttgtcctg aatatgaata cgctccagat actatattct tagctgcaaa atctaagtct
     7501 gcatcttcta atactatagc cgcatctttt ccaccaagct ccaataataa tggtaccatt
     7561 ccagatattt tagatatatg ctgaccaact tcagtactac cagtaaagtt aataaaatta
     7621 attcctttat gcgttacaac ataatcccct atctcactac ccctacctgt aatagtatta
     7681 agtactccat ctggtatccc agcttttctc ataatttcta ccacatgaag tgcgctaata
     7741 gccccttgtg tagcaggttt taatacgact gcatttccac caataagtgc tggagcaatc
     7801 tttgacattg ataaatttac aggataatta aaaggagata tagctaaaac agtacctaat
     7861 ggaacccttg aaacataaga catcttattt cttgttccac cagggaaatt atctccacta
     7921 atggcttcac cctccatatt ttttcccaca tcagctgtgt atcttaaaaa atctgctgtt
     7981 ctctcaactt ctgcccttgc agattttatg tctttggcta tttcaagtac cagtatatta
     8041 gctatctcat ctatattttc taacaataaa tcagcagcct tatgaaatat attggctctc
     8101 ttgtatacag gcatttcagc ccatactttt atactctctt ttgtgttttt tataacctca
     8161 tctacctcat gttttgtcat agattgtact ttcccaacaa gagaattatc tattggtgag
     8221 taaatttcta ttggctttct cgaattagat tctacccatt ttccatttat taaattttta
     8281 tatacatttt cttttgtttt aagttcatta aacatatttt tattcctctc tctgatataa
     8341 ctttgtgtat ttaaattata gccatttatt cgttatgtaa acatttcttt ttttcataaa
     8401 cgtttaccta taaaaaaaat ttaaagttaa cgtttttcta cattcttcta ttttaataac
     8461 atactcgctc caaatctata taggtcacac tttaatacta atataccaat atactaatat
     8521 atcaatatac caatattata aacataaatc acttgacaaa tttataagta gacaatatta
     8581 tatatctatg acttaaaata tttttaaagt catagatatc atatatagac agaaaggatt
     8641 gataaaaatg gctaaaagtt ttacagaaat agaaaaagac aatataagaa aaagactaat
     8701 ttcagaatgt gaaatcaact ggtctaaata tggatacaaa aaaactaata ttgatgaact
     8761 ttgttcaaaa tcaggaatcg caaagggttc attctacaca ttctttgatt caaaagaaaa
     8821 gttatttttt gaagtactaa atgaaataca aaacaaactt atatcatcag tagatgaaat
     8881 tttaagtaat atgcctccaa aagaaggctt tgcaaaatct ttaaaaatgt tatatcgatt
     8941 atatgataaa aatccatttt tatatttacc aaataatcat gattacatca gcctaataaa
     9001 taaactacct caagatatgt tttcacaact tgaatacaat aatatacaaa gtttttatga
     9061 cttaattgat aaatacaatc tcaagtttaa gatagaaaaa gaaaaagcct ttggtatttg
     9121 tgttgctctg ctatcaggtc tattggtaaa agaaaatatt gaatataacc atattgaagt
     9181 gtttgatttt atggtggata atcttgtaga acacatactt gaataacaat ttaaaataat
     9241 ttttctaagt atttttttat actaaatagg attaatatga ttgaattaat gcaaaaaata
     9301 aacaatataa tttataaaat aaatgataaa ctaaatatca cacaaaactt atagacccta
     9361 gagcaaaccc attccactct actctaagcc atctataata gtttgtatat ttctaataat
     9421 attagatatt aaagtaagaa aaaaattcct tataataatt gctattatat caattattat
     9481 aaatatttta ataatcttag ataggtaggt acatatatgg taaaatcctc gaacactttt
     9541 aataaaaact tcaacttgat gatattaggt caaataatct cattgtttgg tgcatcaata
     9601 ctcaagtttg ctttgtcttt atacatactt gatataactg gtaaagcaga aatatttgct
     9661 actatactag cagtatcttc aatcccaatt atagtattct cccctattgg tggagctatt
     9721 gcggatatat tcaacagaag aaacttaatg gttatttttg atttttcaag tagtcttaca
     9781 gtactaatct tagcagtatt cttattcaac aataatggtt caatattact tgtaggaatt
     9841 attatgacca tattatcagt cataagcaca atgtatcaac caactgttca gtcaagtaca
     9901 ccccttttag ttgataatga gcatttaatg aatgcaaatg gtattgtagc tggagttgca
     9961 tcccttacaa atattgcagg acctgtattg ggaggtgtct tgtatggtgt gattggaatt
    10021 aatgcaatta tagttataag ttgtgtaagt ttctttctat ctgcaattat ggagatattt
    10081 atacagattc cttttatcaa acaagagcaa aacagcaaca ttataaaaac tatatttact
    10141 gatataaagg atggtattgt tcatatatgt aaagaaaata gatttatact aaaaatactt
    10201 tttttagcta tagcaataaa cttattttta acttctatgt taattgtagg tatcccctat
    10261 atagtaaaaa taactatggg agctagtaac ttcttatatg gtctagcaga aggagcaatt
    10321 tcttttagtt cactagtagc tgctgtgagt attggtattt tctcaaaata tctaaaagtc
    10381 actaatttgc atattccctt tatcatatgt gcacttatat tcgtaccaat ggcagtttca
    10441 gtatatccat ttatattaaa tataggattt ccagttccat ttttaatctt tatagtatca
    10501 tctatgctaa tattttttac aacgagcctt acctcaatat atgttataac aatcacacaa
    10561 ttaaaaactc caaatgaact tttaggaaaa gtaatgtcaa ttcttttttc aggaacatca
    10621 attgcaatgc cccttggtca ggttatgtat ggtaaacttt ttgatttatt atcagataga
    10681 gtgtacatca ttatactagg agttggattt attacactgc ttatagcatt tgtatcaaag
    10741 ataacattga ggaacgaaaa tctttagata caaagaattt agatataaag atttaatttt
    10801 cactaatttc tgaatttaaa tgcaaaattg tgtatcagta tgttgacgtt ttagaaattg
    10861 attgatataa tcaatggaaa ataaacttta taaaaattca aaatattcag ctagaggagt
    10921 gataaaaatg agttacatgc caactttaga acaagcatgg gaaatattaa gaaaatacaa
    10981 caaggaagaa tttcatataa gacatgctca aattgtatct ggagttatga gatactatgc
    11041 aaaggagtac gaccctgaaa gagtagaatt ttgggaaata gttggacttt tacatgattt
    11101 agattttgaa atgtatcctg atgaacattg tgttaaacaa agagaattaa tgacagagct
    11161 tgatttagat aaatcaataa tcgattctac aataagtcat ggatatggat taactggttc
    11221 agatgttaaa ccagagcaat ttatggagaa agtattattt gctgtggatg aacttactgg
    11281 tttaattggt gctgctgcaa taatgcgtcc ttcaaagagc gtttctgatt tagaattaaa
    11341 atctgttaag aagaaattta aagataaatc atttgctgct ggctgttctc gagacgttat
    11401 aagaagtggt gcagaaatgc ttggatggga acttgataag cttatccaaa caactataga
    11461 agctatgaga agcttgattc cagagatggc aatatagctc ctccatacat ataccataga
    11521 agttacatct ttaaagagca tttctctttt tgatgcaact tctttttata ttttgtagaa
    11581 taaaaagttt aatattttaa actcactata taatctataa taaatactat
//
LOCUS       DACXMZ010000118        11586 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.119, whole genome shotgun sequence.
ACCESSION   DACXMZ010000118 DACXMZ010000000
VERSION     DACXMZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.119"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            70..768
                     /locus_tag="JD016_12120"
     CDS             70..768
                     /locus_tag="JD016_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000379.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            946..1836
                     /locus_tag="JD016_12125"
     CDS             946..1836
                     /locus_tag="JD016_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000380.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLEKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGVSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(2236..3192)
                     /locus_tag="JD016_12130"
     CDS             complement(2236..3192)
                     /locus_tag="JD016_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAU5000381.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIQFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3895..4890
                     /locus_tag="JD016_12135"
     CDS             3895..4890
                     /locus_tag="JD016_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAU5000382.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGTGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            4913..5731
                     /locus_tag="JD016_12140"
     CDS             4913..5731
                     /locus_tag="JD016_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000383.1"
                     /translation="MFYKFKQSIRRYYEVAKINFKVQLIWRFDVVVNMVFTIVKILFA
                     YILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSEEICNRVKLGTFSKYM
                     IIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFTSNPVQILISILMIFL
                     GFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPLVLLPETIVTVMKVFP
                     FYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYKRMRTLFDGVGI"
     gene            5743..6522
                     /locus_tag="JD016_12145"
     CDS             5743..6522
                     /locus_tag="JD016_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000384.1"
                     /translation="MKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLMFNSIY
                     SHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYITKPLDP
                     LFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLLFYDVC
                     IILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMSTIPTQ
                     ILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            6807..8186
                     /locus_tag="JD016_12150"
     CDS             6807..8186
                     /locus_tag="JD016_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAU5000385.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            8634..9347
                     /locus_tag="JD016_12155"
     CDS             8634..9347
                     /locus_tag="JD016_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000386.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(9619..11157)
                     /locus_tag="JD016_12160"
     CDS             complement(9619..11157)
                     /locus_tag="JD016_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAU5000387.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         4222 a         1326 c         1767 g         4271 t
ORIGIN      
        1 tgatgataaa atataattaa gctaagttgg tttttataaa cataaattta ttttagagag
       61 gtgaaataaa tgaaaatatc tagtcaatat agaagtcaat attcatttag atatgaaagt
      121 aatataaata atacaagaat aaatgaaagt atggttaaga aaaatgaaac tgtaggaaaa
      181 gacacttatt tatctaatat tatgaaacaa aagcaagaac ttaatgatag aattagagat
      241 ttaaaatata gacaagaggt ttatactaaa aaaataaatg acgcaattaa gaacttatgt
      301 aaatcagaaa taagagaaac aactaataac ttttctaata tagaaatagg tattaaaaat
      361 agcattatag aagataaaaa taaaagtaca atgttagatg aaaattcaac ttatctaaat
      421 acaaatgatg aaaaagaatc tttaatcact aaagagtcta atgaaaaaat tgaagaagaa
      481 atattaaatg atgaaaaatt agaagagtta gaacaaaaaa aggattataa agaggattct
      541 aataaaaaag agaaagtatc tgaggactta tctttagtag gtaaaactcg tgaagagctt
      601 gaaaatatgc ttaaaaattt tataaattta acacaagaag aaataatgaa acttgagtcg
      661 agaatagaaa agttagataa agatgctgaa gaatacaaac aaaattcaaa gactaatata
      721 tttgataaaa cagatgaaca aaaaaaacat ataaatgtac tgatttaaac aatatgtttt
      781 aaaaaaataa gtttattata taatgaaaat ataaaggtac aatttcaata cacttctatt
      841 aaaccaataa tttatgaatc agacttaatt tcaatttact aataaccata tatttatgag
      901 ttaataattt tatgttttaa aaagaattaa ataaggggga gtagtatgaa gaagaatata
      961 ttaactacta tgattaccat gattattata ttgatgatga gtatgacagt aatggcagag
     1021 ccatatcaag aagatgaaac atctagcttg gaaaaacaga gtgcaaatga acgtaatcag
     1081 actaatatag gcacaatgga tggcagagtg caaacaaatg caataagtaa aggtgctaat
     1141 tatgacccta aatatcctct taaaggaatg ttaacacaac ttggtcttaa tacaacaggt
     1201 aaagagggat gggatttact ctatggagga aatcccaatg gagttgagct tcgagcagat
     1261 ggtaaatatt attatataaa taagggtatg gaagggctag gattttatgc ggcagcattg
     1321 tcaggtgtct catatcagag aataggcttt ggaaaacttg accctataga agtaatagct
     1381 gatggaaatt ggatttctta caaacaagct caagatacac ttacagtaat caggaatttt
     1441 ttaaatagtt ttgactggag aaatgcatct gagatggaaa gagctaatag agctgcaaaa
     1501 ttggtaaccg aagcaaaata tgcagactct aaatattgta acatagtcta tggtaatctg
     1561 gttgataaac gtggtgtatg tggaagtttt gcaagtagct ttcatttatt aacaaggtta
     1621 atgggaatgg attcactatc tatattaaat ccatcattaa atcatgcttg gaactatata
     1681 caaattgatg ggaaatggta taggtttgat gggtctgaaa tatcagcttt tggtggatca
     1741 ttagactttg attaccgtaa attaaaggat gcaacaagag agatgactac ttactatgat
     1801 gctaaggcat tgtctatatt aggatttaat caataaaaat agggaaggct tcttatggag
     1861 ccttttttat tgatttaaaa tttaataagg aattttatat gaaaatttaa tcaataaaag
     1921 caaaaaaggc tctttataaa gcctttctaa atgttttttt aagtaaaatt ttttatatca
     1981 acatcagttt taatcaaaaa atataacatt ttggatttac ttttaaaaat aaaattatta
     2041 aaatgtaata ttaatcatgt taataatgtt tttagtttat tatattctat aatagtggtc
     2101 atgttgacta ttgtagtttt attaaaaata gttggtttat ataaatatat gcaaagatag
     2161 cttgttgttt actttaccta ttagtatata ggacaagata ctctaataaa tttgcctatt
     2221 ttaatatctt ttacactaaa accaactaaa gtttccttta ttatttcgtt ttgaataata
     2281 caaagcataa tccgattgtt tgtataaaga ctggaaagta tcagagttat tttctaattt
     2341 agatatacct atagaacaag aattactctc taggcataat tctaaaactt tctttgcttt
     2401 attttcaaca ttctcttttg attgattact atattcaaaa acagcaaatt catctccacc
     2461 aagtcttcca ataatagcat catttacgaa tactttagtc aaaatatttg aaaattgtat
     2521 tagtatttca tctcctcgaa catgaccaaa gatatcattt acctgtttaa aattatctaa
     2581 atcaaagatg taaaagtatc catgtgaaac agttttaagc atttctttta tgtgtacttc
     2641 aaaaaaatgt ctattgtata gtccagtcat aaggtctttt tctgatttta gtttaaattc
     2701 atttgccatt gctagtgctt cttgtgcttt ttcagttatt gttttagggt aatactcatc
     2761 tttcatttgg tcaaagtttg gaatagaatg gtgaacatgg ataagtttaa tcatatcatc
     2821 tgtaactctg tataccatag taaatcgagt atccatttct acatagatat ctttttctag
     2881 gtaatctttt tctattaccc aaagagtacc atatactata aaagcttcgt ctgtgacttt
     2941 ggtacatgaa taccactcat ctataatctc aaagtctatt tgctctgatt ctgtttggtc
     3001 tttttcaaga cctataataa attcttctaa tgtttcgtaa aattcatgtt ttcctgttcc
     3061 aattaatgca aggttgtcat ctaataagga aataataaat ttaaggtttt cttcttctgg
     3121 atttaaaata taaatatgcc aaattttttt tgataaatct cttaattgtt tttgtattgt
     3181 attattttcc atagattacc cccttaagat ttacagttta tattttaatg tattataagt
     3241 tgaactctta ttacaatatt attataatat agaactaaag aaattacatc ctgtataggt
     3301 aaaagtatat cttttacaat aaaaatatta cagtaaaatg accagtttta atatacgtat
     3361 tatattttta atttataaac gaatattata aatgaaattt aaattcaacc ttgaagaaat
     3421 aagctaaatg tattagtata taaatccatg cttgaacata aataaccttt atgatatgat
     3481 atcaataata atattatgag aggagtgcct tgatgtcgga gttatataat ggtaaccaac
     3541 aattaaatag ttggagacac atatattctg tataatcaga aatttcaagt gtatttttaa
     3601 tacatttaat ttttgtgtct tattctgcct ttatagataa ctgacatatg acaacaaata
     3661 gtatttaaaa tatatattac tttgttaaat tatggtgtag taggctttaa tctgctacac
     3721 ttttattaat tttgtatagt attattttga taactatttt gaaatagaga gcattaattt
     3781 ttctagtcca caggagtttt ctgtttttgt gctgttaaat ataagcacag aaaaaggcag
     3841 atatatacct gtggactttt tttatataaa ttatgaaata tggaggtagt taaaatgagt
     3901 ataattgaac ttaatgaact gactaaatct tatagaagat ttgagaaaaa ggcaggattt
     3961 atggggagtg taaagtcttt atttaatagg aaatatacag aaaaaacagc tatatcgtca
     4021 tttgatttaa agatagaaga aggagaattt gttggattaa taggtcaaaa tggaactggc
     4081 aaaactactc ttattaagat gttaactggt attattgcac cgtcttctgg agaaatatct
     4141 gtaatgggtt attaccctaa caaattagaa aataatttta aaaaacaata tgctgttgtt
     4201 atgggccaaa aaagtcaact attctttgaa ttgacaccag cagatacgtt tcttttattt
     4261 aaagaattat atgatatatc taatgaagaa tataaaaaaa acttggatta ctttatcgaa
     4321 ttatttgagg tatctgacta tttaaatgtt caagttagga cgctttcttt aggagaaaga
     4381 atgaaaatgg aactaatagt agcccttcta cataatccaa aaatattatt tttggatgaa
     4441 ccaactatag gcttagatgc tatagcccaa aaacaaatta ggtatttcct aaaagaagta
     4501 aatgaaacaa aaggaactac tattatactt acttctcatt acatggaaga tattaagagt
     4561 ctctgtagta gaagtattgt tgtagaaaat agccgtaaaa tatatgatgg ttctacagag
     4621 aaactattta atcattttca aaaatataaa aaaataacag ttagtttctc caatgaaact
     4681 aacattgtat ttccaaaaga ttgtaaagtt gttgaacaaa caaatcataa gatatcagta
     4741 attgtgccaa aagaaagagg aaatgaaacg attaaatgga tttttgataa atatgatgtt
     4801 aaagatgtaa tgattgaaga agaagatata ggtagtattg tagagagaat atataagaaa
     4861 ggaagtgatg agtattatga gcaactataa tgaagcaagt tcaaattatt caatgtttta
     4921 taaatttaaa cagagtatta gaaggtatta tgaagtagcc aaaattaatt ttaaagtgca
     4981 acttatctgg agatttgatg tggtggttaa tatggtgttt acgattgtaa aaattttatt
     5041 tgcttatatt ttatggaaag caatttttgg tgaaaataaa atagtctctg gttttacatt
     5101 taactctatg ttgtcttact atataataag ctcattttta gcaggtattg atatgtcaaa
     5161 gagtaccagt gaagagatat gtaatcgtgt aaaattgggc accttttcaa agtacatgat
     5221 tattccaatt aatgttcaaa attattttat tgctaaaaat tttggaatga catgttttta
     5281 ttttgtattt aatatgattt ctgcatttgt atggattttt atatttaaaa tagattttgc
     5341 ctttacatca aatccagttc aaatattaat ttcaattttg atgatttttc tagggtttat
     5401 ttttatgatg aaactccact tcttcattgg gattttgtca tttaaatttc ttgagattag
     5461 tttttttaga atgataattg aaaatattgt tgaatttatt acaggaacaa tgataccact
     5521 tgtactctta ccagaaacta tagtaacagt tatgaaggta tttccatttt attatacaac
     5581 atatttgcca tctatgttac taataggtag aaacagcaat gaggcaatac aaggattaat
     5641 tattctagca ttatgggttt tatttttttc tgtagtaaat aaagttatgt ataaaagaat
     5701 gagaacatta tttgatgggg tgggtatatg atgaaaaaaa atttgaaaat aatatttatg
     5761 ttattaaaaa tgaaattatc aaagatgatg gtatttagat tcgatttttt tggagcattt
     5821 tttgtagata gttcattatt tatattacaa cttctaatgt ttaatagtat atattctcat
     5881 gtagactcaa tagggggatg gcaacaagga gagatgttaa tatttatagg aactttctca
     5941 ttaataaatg cgataaatat gacgattttc ttttttggta tttatgatat tccacgaaaa
     6001 atccaagaag gagaattaga ctactatatt acaaaaccat tagacccact atttaggtta
     6061 acatttgaaa atattaatat tggttcatca ccattaattc tagcaagtat acttattgtt
     6121 ttatatggaa tatctgagtt aaactcagag ataactttag ttatgataat aatatatatt
     6181 ttcatggtgt tattgatgac attacttttt tatgatgtat gtatcatact tagaacagtt
     6241 tcattttttg ttatatcatc ttctgcgata atgagattag aagataattt attgccaatg
     6301 aatatgaaaa ttcctggtgt aatatatgaa ggtgttttta aagtattatt ctatttaatc
     6361 ttaccatatg gaattatgtc gacaattccg actcaaattt tggctgggac aataacaatt
     6421 aatggattaa tatactcaat tttaattgta tttttgttta cattgtttat gtggagattt
     6481 tggaagttag gtatgagaca ttataaaagt gcaagtagtt aataaattag taaaaataaa
     6541 caatgtataa ctatatataa atacgtttca tatgacacta aactatgtga acaagtattt
     6601 gcgctaaagg cactgaaagc tataaaatta ttaaagtaag gatttaaaag tttctttagt
     6661 agaaatataa ttgtgtaaaa aagcaagatt aattaaaact atatccaaat aattagatat
     6721 aattcttatg cagaaaggag gtctgtaaat gtctaattgt ttggaatata ttttaaaaat
     6781 aattaatgta cagaaaaata aacaaaatga gtgatgtata ttattcagaa aatcatgaac
     6841 aagataatct ttatgagcaa gataatttac tggcacaaag atttaattta tttactctat
     6901 tgaagtttgc atttcctact atttttatga tgatttttat ggggttgtat acaatcgtag
     6961 atactatatt tgtatcaaga tttattaata caaatgctct atcagcaatt aatattgtat
     7021 gtccaattat taatcttata gttggaattg gaactatgct tgccactgga ggaagtgcta
     7081 ttgtagcacg taagatggga aatggtgata ttgagggagc aaataaagct ttttctttaa
     7141 tcgtattatc aggagttgta attggtgtaa taattactgg tataggtatt gtatttatgg
     7201 attatatcat tatatggtta ggagcaagtg aagttttaat taaatattgt cgttcatatc
     7261 tttttattat cttggttttt gcttctgcaa atatactaca agtattattt caatgtttat
     7321 ttgtaacagc tggaaagcct caatttggat tatggctagt gttaggtgct gggtgtgcta
     7381 atataattct tgactatgtg ttcattgttc tgttacaaat gggtattagt ggagcagcac
     7441 ttgcaactgg agtaggatac ctaattccta ccgtttcagg tatggttttc ttttcaaaaa
     7501 ataaagggtt acttaagttt tgtataccta gactagataa aagaatatta tttgaagctt
     7561 gttttaatgg ttcatctgaa atggtgggtc aactatcaac agcagttact acttttttat
     7621 ttaatgctat gatgatgaaa ttaattggtg aagatggggt tgcagctatt acaataatta
     7681 tttattctca atttctttta acttcgcttt atataggttt ctcaatgggt gttgcaccaa
     7741 taataagtta taactatgga agtgaaaata atacgcagtt aaatcatgtt tttaagattt
     7801 gtatattatt tatttcagta gcatctttat tggtatttat cttttctata tcatgttcac
     7861 caatcattgt agagattttt tctcaaagag gaagccgagt ttatgaaatt gcaaggtctg
     7921 gattttatat attttctttt agttttattt tttgtgggta taatattttt acttcgtcta
     7981 tgtttacagc attatcaaat ggaaaggtgt ctgcaatatt atcttttcta cgtacatttg
     8041 gatttataac tataggttta ttgatattgc caaatatatt aaatataaat gggatatggt
     8101 tagcagtacc aagtgctgag attttaactt tatttttaac cattttttgt atatgtaagt
     8161 ataagaatag atataaatat atgtagatat taaataaata gtttaggtag atattttatg
     8221 ttttaataag taattcaatt agtgggagat atatactatt gaaaattaaa gttataattg
     8281 atataaagaa ttaataaaaa taacatataa aagtttaaaa gggagttgaa cttaaagttt
     8341 gattagaaaa agtcaatttt aagacaactc cttttatttt actattgtat taatctgtat
     8401 ctgtaagtgt attagtctaa tattataaac tttggatgaa attaattttc tataggtttt
     8461 aatttatcat acactttatt ttaaagaaac ttatcttaat tttcatagtg attgaatttg
     8521 tctattagag atgttactat tagtaatgta aaagcaagag tagtgaatat aaaaaacaag
     8581 atagtgatac tgcaagatta gaatataata aatttttatg gagagatttc aatatggata
     8641 catgttttaa taaaaaaata ttacttgttg atgatgaaaa ggatatagta gatttaattg
     8701 aagaagtact cataaatgat ggttttaaaa acattattaa agcttataat ggtctagatg
     8761 ctatttcgct gtgtaagata gcatgtccag atgttgtcat tcttgatata atgttaccag
     8821 atatagatgg tatagaagtg tgtaaaaaaa ttcgagagtt ttcatactgt tctattcttt
     8881 ttttatcatc aaaaaatgat gatattgaca aaatactagg tcttagtagt gggggagatg
     8941 actatataac aaagccattc agtccaagag aaattgcttt tcgagttaaa gcgcaattac
     9001 gtagacaaca gtatcaaagc attgtaccat cagatagtga agttattaaa ataggagata
     9061 ttactattga tatagaagga aaccgtgtat ataaagatag aaatgagatt gagttaactg
     9121 gaagggagta tcatctttta agctacatgg ctcaaaatgt aaataaaatt ataggaaaag
     9181 aaagactcta tgaacaagta tggggagtat atagtagtat ttgtgacaat acaattatgg
     9241 tgcatatacg acatataaga gaaaaaattg aagataaccc ctcaaatcct aaaatactta
     9301 taacagttaa aggactagga tataaacttg tgaatagaat cgattagaat agattttaat
     9361 attaatgaag atatctcaag ttgaattttt agttaatgag gcattctttt atgggtgaaa
     9421 tcaaatgtat tttcgttatc tcaacaatga agaagaggtc tatctctatt ttgagatagc
     9481 ctcattaagt ttatatcctg gtaatgtact ttatgttcta ataaaatttt tttagtttat
     9541 ttgcagtgaa acattaacta gaacattaaa atttgcattg aaataccaat taaataatat
     9601 tgtatattgt gtataaactt atactaattg tttttctata ttaaatactt tatcaatcca
     9661 acctttataa aatttttctt catgggaaac taggattata cttcctctaa actgtttcaa
     9721 agcattttgt aaagcatcct ttgtctctgc atcaaagtga ttggttggtt catccagaat
     9781 aagaaaatta caaggagata acatcataca acataattta accttagatt gttcaccacc
     9841 acttaaagta gatacactcc ttgaaacatg ctcttccttc acaccacatc tggctagatg
     9901 acgtctaatc tcttttgtat ttaattttgg atatttatct gctactattt gaagtggggt
     9961 tttatcagga ttttcccatt ttaaatcttg ctcataataa ccaattttaa cttgttctga
    10021 aaactggaaa tctccagata tacgaggaat atcttttact aatgtcttta acaaggtaga
    10081 tttaccgatt ccattaaaac cagttataac tattttttga cctccatcaa cagaaaaatt
    10141 taattttgga agtaaagaat agtagtatcc aacttcaaga tttgttatag taagagcatt
    10201 ttgagccgat atttcaattt ctgaaaattg aatatttggt tttcctgtaa aactaggagg
    10261 agctattctt tctattttat ccaattgttt ttgtcttcct cttgcaattt ttgaatttac
    10321 tccagcttta tttttacgta taaatgtttc ttctttttct attttctttt gttgtgcttg
    10381 ataccttcta atataatctt ctcttaaatg tgatttttgt ttaaggaact ctgaatattt
    10441 tccatgatat ttcttaatca tgccaaattc tatatcaaga atacctgtag aaattttttc
    10501 taaaaagtca aaatcgtggg aaactacgat aaatgcccca ttaaatgtat ttaaataatt
    10561 tgagagccat tcaacatgtt ctttatctaa aaagtttgtt ggttcatcta ataatagaac
    10621 atcatggtta gaaagtagta attttgctaa aattaccttt gccctttgac ccccactaag
    10681 ctctccaaca acacgattca ttcctattga atctaatcca agaccatttg ctattttatt
    10741 tatttcatta tctatagaat agaaattact tgctattagt tgttcctgat aatctgatgc
    10801 tttgattaga tattgctcat tttcagatat agcacttttt tgatagagta aattcatttc
    10861 cttttctatt ttataaagtt cttcaaatgc agtgtgtaaa tagagtgata ttgttgtatc
    10921 tctattgatt tctgcatatt ggtctaaatg gcctatattg atattaggtt gccacttgat
    10981 ttctcctgaa tctggaacaa cttctcccaa taaaatttta attaatgtac ttttacctgt
    11041 accattttgg ccaactacac ctatatgttc acctttgaat aaatcaaagt tagctttctc
    11101 atacagtgat ttatccataa aacattgtga taaattagtt acttttaata aactcataat
    11161 tcaaactcct tttcttttat taaaaaattt atggaaataa aaaaagcaga ggaatggcat
    11221 tgtaaaatgc cgacctctgc ttttggaaaa acaaaaggtc atagacaagc attgttgtct
    11281 atgaccagaa aatctatatt atctatcggc aaataggtaa ttttaaataa gcatagccaa
    11341 aaaactatac taaacctata tttgccgatg aagatagaaa gctttaagct ttcagattaa
    11401 cctctacaca acgcttcatc gtatacattt gtgtagagtt aagttttcta acaagtttaa
    11461 ctaattctat ataaatcata attattcaca tcctaatctt agtattaatt ctagtttatc
    11521 tatatttaaa ttgtactttt gtactttgtg gattatctta tttatttaaa atatgtttta
    11581 ttaatt
//
LOCUS       DACXMZ010000119        11556 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.120, whole genome shotgun sequence.
ACCESSION   DACXMZ010000119 DACXMZ010000000
VERSION     DACXMZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.120"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..189
                     /locus_tag="JD016_12165"
     CDS             <1..189
                     /locus_tag="JD016_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAU5000388.1"
                     /translation="LVFFKGRDADALTAAFKEFSAKKLTQEQKPSIRKLIVSLKEKAA
                     ALNAQRDKVKNKDRGIAR"
     gene            186..1976
                     /locus_tag="JD016_12170"
     CDS             186..1976
                     /locus_tag="JD016_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAU5000389.1"
                     /translation="MNAINWKKLLLPNIPYLLFVYLFDKVGQAVHLAPGADLSGKVLS
                     LGEGFSAAFANPLPSLAPMDLLIGILGAVLIRLIVYVKGKNAKKYRKGVEYGSARWGT
                     AEDIKPYTDPVFQNNVLLTQTERLSMNSRPKQPKYARNKNILVIGGSGSGKTRFFVKP
                     NLMQMHSSYVVTDPKGTVLVECGKLLQRGGYRIKVLNTINFKKSMKYNPFAYLRSEKD
                     ILKLVNTLIANTKGDGEKAGEDFWVKSERLFYCALIGYIWYEAPEEEKNFTTLLEMIN
                     ASEAREDDPEFQSPVDLMFERLEEKDPEHFAVRQYKKFLLSAGKTRSSILISCGARLA
                     PFDIKELRDLMETDEMELDTIGDRKTALFVIISDTDDTFNFVVSILYTQLFNLLCDKA
                     DDEYGGRLPVHVRCLLDEFANIGQIPKFEKLIATIRSREISASIILQSQSQLKAIYKD
                     NADTIVGNCDTTLFLGGKEKTTLKEMSEILGKETIDSFNTSENRGREVSHGLNYQKLG
                     KQLMTEDEIAVMDGGKCILQLRGVRPFFSDKYDITKHPNYKYLSDYDKKNTFDMEKHL
                     RRRPALVKPDEVFDYYEISESDLQEDTDHE"
     gene            2587..2802
                     /locus_tag="JD016_12175"
     CDS             2587..2802
                     /locus_tag="JD016_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU5000390.1"
                     /translation="MEFFNSAIDVLQTLVIALGAGLGIWGVINLLEGYGNDNPGAKSQ
                     GIKQLMAGGGVALIGTILVPLLSGLFG"
     gene            2821..3690
                     /locus_tag="JD016_12180"
     CDS             2821..3690
                     /locus_tag="JD016_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000391.1"
                     /translation="MESILEAINEWIKEILIGAINGNLSTMFGDVNEKVGTIAAEVGK
                     TPQGWNANIFSMIQNLSENVIVPIAGLVITYVLCYELISMVTEKNNMHDIETFMFFKW
                     FFKAFVAVFLVTHTFDITMAVFDMAQHIVSGAAGVIGGDTNIDVTEALAAMQEGLKDM
                     EIPELLLLVMETSLVSLCMKIMSVLITVILYGRMIEIYAYCSVSPIPFATMTNREWGQ
                     IGNNYLKGLFALGFQGFLIMICVGIYAVLVGEMVLADNLHSAIFSLAAYTVILCFSLF
                     KSGALAKSIFNAH"
     gene            3732..4136
                     /locus_tag="JD016_12185"
     CDS             3732..4136
                     /locus_tag="JD016_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAU5000392.1"
                     /translation="MAYVPVPKDLSKVKTKVAFNLTKRQILCFSVALLMGLPLFFLLK
                     DSAGTSLAAMAMIVVMLPCFLVAMYEKHGQPLEVVVKNIIQTKFTRPKVRPYQTENLY
                     ALLEKQRALEKEVSAIVKRTDKKDAGSRRKQA"
     gene            4084..6513
                     /locus_tag="JD016_12190"
     CDS             4084..6513
                     /locus_tag="JD016_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAU5000393.1"
                     /translation="MSKGQTKKTREAAGNRRKLTRAEKKQIAAVIRQAKGDGKAHTAQ
                     QTIPYLAMYPDGICKVAQHKYSKSIAFEDINYQLAQADDKTAIFENWCDFLNYFDASV
                     NVQLSFINQGSQQEQAARAIHIPPQNDDFNSIRTEYSDMLKSQLAKGNNGLVKHKYIT
                     FSIEADNPAAARARLSRIETDVLNNFKVLGVSAHPLSGYERLKVLHGVFHPAGEPFSF
                     SYDWLTPTGLTTKDFIAPSSFKFGEGRYFAMGKKTGAVSFLEIFAPELNDRILADMLD
                     LETGVIVNLHIRSIDQSEAIKTIKRKITDLDKMKIEEQKKAVRSGYDMDIIPSDLATF
                     GNEAKNLLQDLQSRNERMFLLTFLVVNMADTKRKLENDIFAAAGIAQKYNCALTRLDY
                     QQEAGLMSSIPLGENLIPIQRGLTTSSTAIFIPFITQELFQTGAALYYGLNALSNNMI
                     LCDRKQLKNPNGLILGTPGSGKSFAAKREITNAFLITDDDIFICDPEAEYFALVKRLG
                     GQVIRLSPTGKGMDGTPQYVNPMDMNLNYSEDDSPLALKSDFILSLCELVIGGKEGLQ
                     PVDKTVIDRAVRNVYRPFLADPDPAKMPILGDLYDELLRQPEPEAARIAAALELYVSG
                     SLNVFNHRTNVELNNRLVCFDIKQLGKQLKKLGMLIVQDQIWNRVTINRAEKKSTRYY
                     MDEFHLLLKEEQTAAYSVEIWKRFRKWGGIPTAITQNIKDLLASREVENIFENSDFVL
                     MLNQAQGDRAILAKQLNISPQQMKYVTHTEAGEGLIFYGNVVLPFVDHFPKDTELYRI
                     MTTKPEEVSSL"
     gene            6510..7469
                     /locus_tag="JD016_12195"
     CDS             6510..7469
                     /locus_tag="JD016_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HAU5000394.1"
                     /translation="MKTDVIINRDALCALKELPSESVHCCVTSPPYFALRDYGLDAQI
                     GQEDTPEQYIDRLTSVFRELYRVLRKDGTLWLNIADTYCGTGNKGGYADPKNPKGRTG
                     QRIARNSRVTGCKQKDLIGIPWLLAFSLREQGWYLRSDIIWQKQNPMPESCKDRPTRC
                     YEHIFLLSKEKKYYYDAAAIAEPLAPTTAERYRRARSTNSKYTQEIPGQGKVQGLNRP
                     RDGGYYDDALMPTTRNKRDVWLINTVPYKGAHFAAFPPKLAETCILAGCPKGGIVIDP
                     FFGSGTTGFAAKSLDRHYIGIELNAEYCALARARIGGAGLSNN"
     gene            7496..9436
                     /locus_tag="JD016_12200"
     CDS             7496..9436
                     /locus_tag="JD016_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAU5000395.1"
                     /translation="MTRDGVVEVNETQQTAERISSREADSDFSQPDSAAAERVIEHLD
                     AAHTRKANKKAVKKEQEATALRTSTSRLQFTEEELAAPELERYIEKSNKAADRLDAAK
                     AAIPKQKKLVKERTFDEATGKAKTRLLFEKQEKPIPGGKKGNPLSRPAQEAGIFVHNK
                     IHSVEKDNSGVEGAHKSEELAERGAKYGARKLKQGYRSHKLKPYREAAKAEKAAFKAN
                     VNFQYHKTLHDNPQLTSNPLSRFMQKQQIKRQYAKAAKKGGAKAAAEATRKTAKKTAE
                     ETRKAAAFAARHPAGILIAVAALLLFIMISAGLSSCGAMFSGLTNGVLGTSYTSEDSD
                     LLAVENNYAALENGLQSEIDNIENTHLGYDEYRYDLDSIGHNPHELASYLTALLQSYT
                     PQSAQAELERIFAMQYTLTLTEEVEIRYRTETSTDPETGETTTEEVPYEYYILNVKLT
                     NKPISELAEELLTPQQLEMFHVYLETSGNKPLIFGGGSPDGSPSEDLSGVEFVNGTRP
                     GNQELMELAKQQVGNVGGYPYWSWYGFNSRVEWCACFVSWCYNQAGKSEPRFAGCEWQ
                     GVPWFQSRGQWGARGYENIAPGDAIFFDWDLDGVADHVGLVLGRDGSRVYTVEGNSGD
                     ACKIKSYDLNYQCIKGYGLMNW"
     gene            9468..9737
                     /locus_tag="JD016_12205"
     CDS             9468..9737
                     /locus_tag="JD016_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAU5000396.1"
                     /translation="MANNKIDRINKEIAKTREKITEYQNKLKGLEAQKTEAENLEIVQ
                     LVRAMRLTPQELNAMLSGGGIPGMNAAPATEAADYEEQEENADEE"
     gene            9721..10455
                     /locus_tag="JD016_12210"
     CDS             9721..10455
                     /locus_tag="JD016_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAU5000397.1"
                     /translation="MQMKNKLKKTLTALCAALILMGGFSVPAYAQGAATEPPAEDATN
                     DSGVIVEEKEETPPLTPKGNATLVDDYGGNKQLITVTTKSGNYFYILIDRAAEGEQTV
                     HFLNQVDEADLMALTENGEAAKKPESCNCTEKCEAGKVNTSCPVCVTSMTGCTGKEPT
                     PTPTEQPEEPKEKGGNPGVVLALVLFALLGGGAAFYYFKFLKPKQNVKGDTDLEDFEF
                     EDYDEDEPETDTGETPEDEETEEETL"
     gene            10452..10634
                     /locus_tag="JD016_12215"
     CDS             10452..10634
                     /locus_tag="JD016_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000398.1"
                     /translation="MTLFTDNPFEKMMIQKPNGRRDNAPPVPYPPACTSCPYKGQSPC
                     VGYCIRKLETKRQTER"
     gene            10724..11044
                     /locus_tag="JD016_12220"
     CDS             10724..11044
                     /locus_tag="JD016_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein TfoX"
                     /protein_id="HAU5000399.1"
                     /translation="MASSKEYLEFILGQLSELEEITYRAMIGEFIIYYRGKIVGGIYD
                     DRLLVKPVKSAISYMPTAPYELPYEGAKEMLLVDEVDNKEFLTGLFHAMYDELPAPKP
                     KKKK"
     gene            11165..>11556
                     /locus_tag="JD016_12225"
     CDS             11165..>11556
                     /locus_tag="JD016_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAU5000400.1"
                     /translation="MILVIAEKPSVAQTIAAVLGAKEKKDGFLTGSGYIVSWCVGHLV
                     GLAEAAAYGEQYKKWSYDSLPILPQEWKYTVAADKEKQFKTLKELIHRADVSEVVNAC
                     DAGREGELIFRFVYEMAGCKKPMRRLWIS"
BASE COUNT         3335 a         2760 c         2939 g         2522 t
ORIGIN      
        1 cttgttttct tcaagggacg ggacgcggac gcactgaccg cagctttcaa ggagttttcc
       61 gcaaagaagc tgacgcagga acaaaagccc tctatccgaa agctgattgt ttccctcaaa
      121 gaaaaggcgg cggctttgaa cgcacagcgg gacaaagtaa aaaacaaaga cagggggatt
      181 gcaagatgaa cgctatcaac tggaaaaagc tgctgcttcc caacattccc tatctgctct
      241 ttgtgtatct ctttgataaa gtcgggcagg cggtacacct tgcgccgggg gctgacctgt
      301 ccggcaaggt gctgtcgctt ggggagggct tttccgctgc ctttgcaaac ccgcttccga
      361 gccttgcccc tatggattta ctgataggaa ttttaggcgc ggtacttatc cggctgattg
      421 tctatgtcaa aggcaagaac gcaaagaaat atcgcaaagg tgtggaatac ggctctgccc
      481 gttgggggac tgccgaagat attaagccct acaccgaccc ggtatttcaa aataacgtgc
      541 tgctgacaca gacggaacgg cttagcatga acagccgccc gaagcagccg aagtatgcaa
      601 ggaataaaaa tatccttgtt atcgggggaa gtggcagcgg caagacgaga ttttttgtga
      661 agccgaattt aatgcaaatg cactcaagct acgttgtaac cgacccgaaa ggtacggttt
      721 tagtcgagtg cgggaagctc ttacagcggg gcggctaccg gataaaagtg ctgaacacga
      781 ttaacttcaa aaaaagcatg aaatacaatc cctttgccta tctccgcagc gagaaagaca
      841 ttttgaaact ggtaaacact ttgattgcca acaccaaagg ggacggggaa aaagccgggg
      901 aagatttttg ggtaaaatcg gaacggctct tttactgcgc ccttatcggc tacatttggt
      961 acgaagcccc ggaggaagaa aagaacttca cgacgctgct tgaaatgata aatgccagtg
     1021 aagcccgcga ggacgacccg gaatttcagt cccccgttga cctcatgttt gaacggttgg
     1081 aggaaaaaga cccggaacac tttgccgtcc ggcagtataa gaaattcctg ttatctgcgg
     1141 gaaagacaag aagctctatc ctcatttcct gcggtgcgcg gcttgcaccc tttgacatta
     1201 aagagctgcg cgaccttatg gaaaccgacg aaatggagct tgacaccata ggcgaccgca
     1261 agaccgccct gtttgtcatt atcagcgata ccgacgatac ctttaacttt gtagtatcaa
     1321 tcctttacac gcagcttttc aatctccttt gcgacaaggc agatgatgaa tacggcgggc
     1381 ggctgcccgt ccatgtgcgc tgtctgttag acgagtttgc aaatatcggg cagataccga
     1441 agtttgaaaa gctcatagcc actatccgaa gccgggaaat ctctgcttcc atcattttgc
     1501 agagccagtc gcagctaaag gcgatataca aagataacgc tgatactatc gtcggcaact
     1561 gcgacaccac tcttttcttg ggcggcaagg agaaaaccac cctcaaagaa atgtccgaaa
     1621 tcttggggaa agaaaccatt gacagcttca acacttccga gaaccggggg cgggaggtat
     1681 cgcatgggct gaactatcaa aaattaggta agcagcttat gacggaagat gaaattgcgg
     1741 tcatggacgg cgggaaatgt attttacagc tacgaggggt gcgcccgttt ttctctgata
     1801 agtacgacat aacaaagcac cccaactata aatacctgtc tgactatgac aagaaaaata
     1861 cctttgatat ggagaagcat ttaaggcgca gacccgccct tgtgaagccg gacgaagtat
     1921 ttgactacta cgaaatcagc gaaagcgatt tgcaggagga caccgaccat gaataaacgt
     1981 atcagaaaga aaaaggacaa gaaaatcaca gaagccataa acgggcttgt tttcattgcc
     2041 gagcgacggg aacagcaacg gcaggcagct atccggcagt ttgtgaaacg gtgtgaagcc
     2101 ctgtatgagc agaaacgggt gcggcgggaa ctttgatttt acgggaggtt acactatgag
     2161 cagacagaaa acaaaaactg atgatatacg tcgtgaacat tattttacaa agatgataga
     2221 aaatgcagaa aacgctctgc gggaggattt gcaggaacgc ggaggggaac agacccttta
     2281 cgttaccgag tatatgcagc gcaggagcta tcttttggaa tgacgaaaaa tgtgtgttcc
     2341 cggctgaaaa gaaaggagga attgacgcag taacaaagaa acagacaact tacagaatac
     2401 gcgcccctac tggaaaatcc ataacgccca cactcaaaac tgaataccgc cgcgcggcag
     2461 acacataggc agcgcgggga acccgccaca atccggcggg cttatgaccg cggcagttac
     2521 aaaactgacc gccgtttttt atgccctttt atagggcaag ccgcatttgc ggaagaaagg
     2581 agctttatgg aattttttaa ctctgctatc gacgtattac agacccttgt tatcgcactg
     2641 ggcgcaggct taggcatttg gggcgttatc aatctcttgg agggatatgg aaacgacaat
     2701 ccgggtgcga aaagtcaagg cattaagcag ctcatggcgg gcggcggcgt tgcccttatc
     2761 ggcactatcc ttgtaccgct tctttccggt ctgttcggat aaagttacgg agggtagcct
     2821 atggagagca tactggaagc gattaacgaa tggataaaag aaatccttat cggagccatt
     2881 aacggcaacc tttccactat gttcggggac gtaaacgaaa aagtaggcac cattgccgcg
     2941 gaggtaggca agaccccgca ggggtggaac gcaaacattt tcagcatgat acaaaacctg
     3001 tcggaaaatg tgattgtacc cattgcgggg cttgtcatta cctatgtcct ctgttatgag
     3061 cttatcagca tggtaacgga aaaaaacaat atgcacgaca tagaaacatt catgttcttt
     3121 aagtggtttt tcaaagcctt tgttgccgtt ttcttggtaa cgcatacctt tgatataaca
     3181 atggcagtct ttgatatggc gcagcatatt gtgtccggtg cggcgggggt aatcggcggc
     3241 gatacgaata tcgacgtaac cgaagccctt gccgccatgc aggagggact aaaagatatg
     3301 gaaatccccg aactactgtt acttgtcatg gaaacgagcc ttgtaagcct ttgtatgaaa
     3361 atcatgtccg tactgataac cgttatcctc tacggcagaa tgattgagat atacgcctac
     3421 tgttcagttt cccctatccc ctttgccacc atgacaaacc gggaatgggg acagattggg
     3481 aacaactacc taaaggggct gtttgcactg ggctttcaag gcttcctcat tatgatatgc
     3541 gtcggcattt acgcagtttt ggtaggcgaa atggtcttag cggataacct gcacagcgcg
     3601 attttttccc ttgcagccta tacggttatc ctctgttttt ccctgttcaa atccggcgca
     3661 ctggcaaaat ctatttttaa tgcacattaa cacagcacac gcgggcaagc cccgcagaaa
     3721 ggaggttttt cttggcgtat gtacctgtac ctaaagactt atccaaagtc aaaacaaaag
     3781 tcgctttcaa tctgacaaag cggcaaatcc tttgtttttc agtggcactt cttatgggac
     3841 tgccgctttt ctttttgctc aaagacagcg cagggacgag ccttgcggca atggcaatga
     3901 ttgtcgttat gcttccctgc ttcttggttg ccatgtatga aaagcatgga cagccccttg
     3961 aagtggttgt aaagaacatc attcagacaa aatttacccg tcccaaagtg cgaccgtatc
     4021 agacggaaaa cctatatgca ctcttggaga aacagcgggc attagaaaag gaggtatcag
     4081 cgattgtcaa aaggacagac aaaaaagacg cgggaagccg caggaaacag gcgtaagctg
     4141 acccgcgcgg aaaagaaaca gatagcggcg gttatccggc aggcaaaggg ggacggcaaa
     4201 gcacacaccg cacagcagac cattccctac cttgccatgt acccggacgg tatctgcaaa
     4261 gtggcacagc acaagtattc aaagagcatt gcttttgagg acatcaacta tcagcttgca
     4321 caggcagacg ataagaccgc catttttgaa aactggtgcg attttctcaa ctactttgac
     4381 gcaagcgtga acgtgcagct ttctttcatc aatcaaggct cacagcagga acaggcagcg
     4441 agggcaatcc atatcccgcc ccaaaatgac gattttaatt ctatccgcac cgagtattcc
     4501 gatatgctga aatcgcagct tgccaaaggg aacaacgggc ttgtaaagca caagtacatc
     4561 acgttttcca ttgaagcgga caatccggcg gcagcaaggg cgcgcctttc ccgtattgaa
     4621 acggacgtac tcaacaactt caaggtgctt ggggtatcgg cgcacccctt atccggctat
     4681 gaacgcttaa aggtgctgca tggggtattc cacccggcag gcgagccgtt ctcattctct
     4741 tatgactggc tgaccccgac cgggcttact acaaaggact ttatcgcccc gtcctctttc
     4801 aagttcgggg agggacgcta ctttgctatg gggaaaaaga cgggggcggt atcgttcctt
     4861 gaaatctttg ccccggagct aaacgaccgt atcttagcgg atatgttaga tttggaaaca
     4921 ggcgtaatcg tcaacttaca tatccgcagt atcgaccagt cggaagcaat caagaccatt
     4981 aagcgcaaaa tcactgacct tgacaagatg aagattgagg aacagaaaaa agcagttaga
     5041 agcggttatg acatggatat aatcccgtcc gaccttgcca cttttggaaa tgaagcaaag
     5101 aatttattgc aggatttaca gagccgcaac gagcgaatgt ttctccttac gttccttgtg
     5161 gtaaacatgg cagacacgaa acggaaactg gaaaatgaca ttttcgcggc ggcgggcatt
     5221 gcacagaaat acaactgcgc cttgacccgt cttgattatc agcaggaagc gggactaatg
     5281 tcctctatcc ctttgggcga gaaccttatc cctattcaga gagggttgac gacgagcagc
     5341 accgctattt ttatcccgtt catcacgcag gagctatttc agaccggggc tgccctgtat
     5401 tatggcttaa atgccctgtc aaacaacatg atactctgcg accgtaagca gcttaaaaac
     5461 ccgaatgggc ttatcttggg aacaccgggc agtggaaaat cctttgcagc aaagcgggaa
     5521 atcacaaatg cctttctcat taccgacgac gatattttta tctgcgaccc ggaagcggaa
     5581 tattttgccc ttgtaaaacg gcttggcggg caagtgatac gcttatcccc aaccggaaaa
     5641 ggcatggacg gtacgcccca gtatgtgaac cctatggata tgaaccttaa ttacagcgag
     5701 gacgacagcc cccttgcact gaaaagcgat tttatccttt ccctctgcga gcttgtcatt
     5761 ggcggcaaag agggcttgca gcccgtagat aagacggtta ttgacagggc tgtaagaaat
     5821 gtgtaccgcc cgttccttgc agaccccgac ccggcaaaaa tgcctatctt gggcgacctt
     5881 tacgacgagc tgctaagaca gccggagccg gaagctgccc gcattgcggc ggcattggag
     5941 ctgtatgttt ccggtagcct taacgtattc aaccacagaa ccaacgtgga actgaataac
     6001 cgccttgttt gctttgatat taagcagctt ggaaaacagc ttaaaaagtt aggtatgctc
     6061 attgtgcaag accagatatg gaaccgcgtt accattaacc gggcagaaaa gaaatctacc
     6121 cgctactata tggacgaatt tcatctgctc ttaaaagagg aacagaccgc cgcttatagt
     6181 gtggagattt ggaagcgttt tcgtaaatgg ggcggcatac cgacagctat cacacagaac
     6241 ataaaggact tgcttgcttc ccgcgaggtg gaaaatatct ttgaaaactc tgattttgtc
     6301 ctcatgctta atcaggcaca gggcgaccgg gctatccttg caaagcagct taatatctcc
     6361 ccgcaacaga tgaagtatgt aacccacacc gaagcaggcg agggacttat cttttatggg
     6421 aatgtggtgc tgccctttgt agaccatttc ccgaaagaca ccgagcttta ccgcattatg
     6481 acgacgaagc cggaggaagt gagcagccta tgaaaaccga cgtgattatc aacagggacg
     6541 cgctttgtgc cttaaaagag ctgccctctg aaagcgtcca ttgctgcgtt acaagcccgc
     6601 cgtactttgc gctgcgggac tatgggcttg acgcacaaat cggacaggag gatacgccgg
     6661 aacagtatat tgacaggctg acctctgttt ttcgcgagct ttaccgggtg cttagaaagg
     6721 acggtacgtt atggctgaat atcgcagaca cctattgcgg cacaggaaat aaaggcggct
     6781 atgctgaccc aaaaaaccca aagggacgca ccggacagcg gattgcaagg aacagccgcg
     6841 ttaccggctg caaacaaaag gacttaatcg gtattccctg gctcttggcg ttttccctgc
     6901 gtgaacaggg gtggtatcta cggagcgata ttatatggca aaaacaaaat cctatgccgg
     6961 aaagctgcaa agaccgccct acccgctgct atgaacatat ctttctgctt tcaaaggaaa
     7021 agaaatatta ctatgacgct gccgccattg cggaaccctt agcacctact accgcagaac
     7081 ggtatcgcag agcgcgcagc accaacagca aatacacaca ggaaataccc ggacaaggaa
     7141 aggtgcaggg actaaaccga ccgcgggacg gtggctacta tgacgacgcg ctcatgccga
     7201 ccacgcggaa caagcgggac gtgtggctga ttaacactgt cccatataaa ggggcgcatt
     7261 ttgcagcttt tcccccaaaa ttagcggaaa cctgtatctt ggcgggctgt cccaaaggtg
     7321 ggattgtcat agacccgttt ttcggaagcg gaaccacagg atttgcagca aaaagccttg
     7381 accgccatta tatcgggatt gaattaaatg cagagtattg cgcccttgcg agggcgcgga
     7441 ttggaggtgc aggactatca aacaattaaa accacgcgat aaaatcacac agaaaatgac
     7501 ccgcgacggc gtggtggaag tcaacgaaac acagcagacc gccgagcgta tcagcagccg
     7561 ggaagcagac agcgactttt cacagccgga cagtgccgca gcggaacgcg tcatagaaca
     7621 tcttgacgca gcacataccc gaaaggcaaa caaaaaggca gtcaaaaaag agcaggaagc
     7681 tactgctttg cgtacttcca cttcccgctt gcagtttacc gaggaagaac ttgccgcccc
     7741 ggaactggaa cgctatattg agaaatcaaa caaagccgct gaccgactgg acgcggcaaa
     7801 ggcagctatc ccaaagcaaa agaaactggt taaggagcgc acctttgatg aagccacagg
     7861 aaaagcaaag acccgcttac tctttgaaaa acaggaaaaa ccgatacccg gcggcaaaaa
     7921 gggaaacccg ctgtcccgcc ctgcacagga agcggggatt ttcgtccaca acaagataca
     7981 ttcggttgaa aaagacaatt ccggcgttga gggggcgcat aagtcggagg aacttgccga
     8041 gcgcggcgcg aaatacgggg cgcggaagtt aaaacagggc taccgcagcc ataagctgaa
     8101 accctaccgg gaagcagcga aggcagaaaa ggcagcattt aaggcaaacg tcaacttcca
     8161 gtatcacaag accctgcacg acaacccgca gcttacctct aacccgcttt cccgtttcat
     8221 gcagaaacag cagataaaac gccagtatgc aaaggcggca aagaaaggag gggcaaaagc
     8281 cgcagcggaa gccacaagaa agacggcgaa gaaaaccgcc gaggaaacga ggaaagccgc
     8341 cgcatttgcg gcaagacacc cggcgggcat tttgattgca gttgccgcgc tgcttctctt
     8401 tattatgatt tccgcagggc tgtcctcttg cggggctatg ttttcgggct tgactaatgg
     8461 cgtgcttggc acttcctaca cgtccgagga cagcgacctt ttggcagttg aaaacaacta
     8521 tgctgcattg gaaaacgggc tgcaaagtga gattgacaat atcgaaaaca cccacctcgg
     8581 ctatgatgaa taccgctatg accttgacag tatcggacat aacccacatg aacttgcgtc
     8641 ttacttgacc gccctcttgc agagctacac cccgcagagc gcacaggcag agcttgaacg
     8701 tatctttgcc atgcagtaca ccttgacgct gaccgaggag gttgaaatac gttaccgcac
     8761 cgaaacaagc accgacccag aaaccgggga aaccaccacc gaggaagtcc cttatgagta
     8821 ttacatcttg aatgtgaaac tgacaaacaa gccgatttcc gagcttgcgg aggaacttct
     8881 tacgccacag cagcttgaaa tgttccatgt ctacttggaa acaagcggca acaaaccgct
     8941 gattttcggc ggcggttctc ccgacggaag cccgtccgag gatttaagcg gcgtggagtt
     9001 tgtaaacggt acgcgccccg gaaatcaaga acttatggag cttgcaaagc agcaagtcgg
     9061 aaatgtgggc ggctaccctt actggtcttg gtacggcttt aacagccgcg tggaatggtg
     9121 cgcctgtttt gtatcatggt gctacaatca agccggaaaa agcgaaccgc gctttgcggg
     9181 ctgcgaatgg cagggtgtcc cttggtttca gtcaagggga caatggggcg cacgcggcta
     9241 tgagaatatc gcaccgggcg acgctatctt tttcgattgg gatttagacg gcgttgccga
     9301 ccatgtgggg cttgtgcttg gcagggacgg cagccgcgtc tataccgttg agggcaattc
     9361 cggcgacgcc tgcaagataa agagctatga ccttaactat caatgtatca aaggctacgg
     9421 gctgatgaac tggtaaagca gccccaaaaa gaaaggagaa atgacctatg gcaaacaata
     9481 aaatcgaccg tatcaataag gaaatcgcaa agacccgcga gaaaatcaca gagtatcaga
     9541 acaagttaaa aggactggaa gcgcagaaaa ccgaagcgga aaaccttgaa atcgtacagc
     9601 ttgtacgagc tatgcgtctg accccgcagg aacttaacgc tatgctgtcc ggcggcggta
     9661 ttcccggcat gaacgccgca cccgctaccg aagccgcaga ctacgaagaa caggaggaaa
     9721 atgcagatga agaataaact gaaaaagacc ctgactgccc tttgtgccgc ccttatcctt
     9781 atgggcggtt tttctgtccc tgcctatgca cagggcgcag ccacagagcc gcccgcagag
     9841 gacgcaacta atgacagcgg cgtgattgtg gaggaaaaag aagaaacgcc gccccttacc
     9901 ccaaagggaa acgcaacact ggttgacgat tacggcggca ataagcagct tatcactgtt
     9961 accaccaaaa gcggcaacta cttctatatc ctcattgacc gcgccgccga gggggaacag
    10021 accgtacatt tcctaaatca agtggacgaa gccgacctta tggcactgac cgaaaacggc
    10081 gaagctgcca aaaagccgga aagctgtaac tgtacggaaa aatgcgaagc cggaaaggta
    10141 aacacgtcct gcccggtatg tgttaccagt atgacaggct gcacaggcaa agaacccacc
    10201 ccaacaccaa cagagcagcc ggaagaaccg aaagaaaaag gtggcaatcc cggtgtagtg
    10261 cttgccttag tcctctttgc cctgcttggt ggcggcgcag cgttctacta ctttaagttt
    10321 ctaaagccga agcagaatgt aaagggcgat accgaccttg aagattttga atttgaggat
    10381 tacgacgagg acgagccgga aacagatacc ggggaaactc ccgaagatga agaaacggag
    10441 gaagaaaccc tatgaccctg tttaccgaca atccctttga aaagatgatg atacagaaac
    10501 cgaacgggcg gcgtgataat gcgccgcctg ttccttatcc cccggcttgc acttcctgtc
    10561 cttacaaggg acagtcccct tgtgtggggt actgtatcag aaaactggaa accaaacgac
    10621 agacggaacg ataaaaagat gtacttgcga tatggaatat gatataattg ttccaaccat
    10681 aggaaatgac agagttaaac aaataagaat ttgaggtgga aatatggcat caagcaagga
    10741 atacttagag tttattttag ggcagctatc tgagttagaa gaaattactt atcgagctat
    10801 gataggagaa tttattattt attatcgtgg caagattgta ggcggtatct atgatgatag
    10861 attacttgtt aaaccagtaa aatcggcaat tagttatatg ccgacagctc cgtatgaatt
    10921 accctatgag ggagcaaaag agatgttgtt ggtagatgaa gttgataata aggaattttt
    10981 gacaggcttg tttcatgcaa tgtatgatga actgccagcc ccaaaaccga aaaagaagaa
    11041 ataaacaact tccggtttgt caaattattt tactatttca tatcgcacaa ttttatacaa
    11101 tcacttgtag gagcaatcga aaaatcggtt gctcttttct tttacccgaa aggaggattt
    11161 atcattgatt ttagttatcg cagaaaagcc cagtgtagcg cagacaatcg ctgccgtact
    11221 tggggcaaag gaaaagaaag acggatttct cacaggaagc ggctatatcg tttcttggtg
    11281 cgtgggacat ttggtagggc ttgcggaagc tgccgcctac ggggaacagt ataaaaagtg
    11341 gagctatgac agcttaccga ttttgccgca ggaatggaag tacaccgttg cggctgacaa
    11401 ggaaaagcaa ttcaagacct taaaggagct tatacaccgg gcagatgttt ccgaagtcgt
    11461 caatgcctgc gacgcagggc gcgagggtga actcattttc cgttttgttt atgaaatggc
    11521 gggctgcaag aaacctatgc gccgcctttg gatttc
//
LOCUS       DACXMZ010000120        11465 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.121, whole genome shotgun sequence.
ACCESSION   DACXMZ010000120 DACXMZ010000000
VERSION     DACXMZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.121"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            410..562
                     /locus_tag="JD016_12230"
     CDS             410..562
                     /locus_tag="JD016_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000401.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHSSAFSGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(854..1558)
                     /locus_tag="JD016_12235"
     CDS             complement(854..1558)
                     /locus_tag="JD016_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000402.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1549..3156)
                     /locus_tag="JD016_12240"
     CDS             complement(1549..3156)
                     /locus_tag="JD016_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HAU5000403.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3410..4588)
                     /locus_tag="JD016_12245"
     CDS             complement(3410..4588)
                     /locus_tag="JD016_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000404.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4814..5308)
                     /locus_tag="JD016_12250"
     CDS             complement(4814..5308)
                     /locus_tag="JD016_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAU5000405.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5309..6070)
                     /locus_tag="JD016_12255"
     CDS             complement(5309..6070)
                     /locus_tag="JD016_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAU5000406.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            6812..9646
                     /locus_tag="JD016_12260"
     CDS             6812..9646
                     /locus_tag="JD016_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU5000407.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            9704..10933
                     /locus_tag="JD016_12265"
     CDS             9704..10933
                     /locus_tag="JD016_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAU5000408.1"
                     /translation="MKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALLIDQET
                     KRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGGSTCKL
                     KDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMIDTYYFN
                     PNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDTKKDFT
                     HYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLGDGNKK
                     NRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGVVSDNE
                     NINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDKIKIKV
                     AKINKKLEIKNSVCFKA"
     gene            complement(11266..>11465)
                     /locus_tag="JD016_12270"
     CDS             complement(11266..>11465)
                     /locus_tag="JD016_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000409.1"
                     /translation="VIKTDGTIVPPDAEKTVKLVFTVTHTASSKTADTAEIDVTVPAK
                     TVSTPTSLLGRIAVNIFNFSK"
BASE COUNT         4094 a         1840 c         1442 g         4089 t
ORIGIN      
        1 actattatat attaaaattt cttcaataat aaattattta gattgttact ttgatatatt
       61 atataatatc acaaaggctg atataaaaag acaaaaaggg caattttttt ataccaaaaa
      121 aaatgttaaa ttgatattat ccatatataa attgatatta tccatatagg tgctatattt
      181 aacaattcta cttacttccc ttacacttat tagcatatag ttattaagag tttgttgcaa
      241 aacaacactt agcacacttt aaaaagtgtg gtctaataag taatcaacga agaagacata
      301 ataaagcata aaatatgata tacttttcca cgtcatcaaa actgaggatt ttattattaa
      361 tatatgataa aatatgctta ataaattaaa ttttaatggg agaatagtta tgaaaatatt
      421 tagtttcttg ttgtttgtag tgggaatatg tttttgtatt gctggattta tggggcattc
      481 ctcagctttt tctggtgcaa cattgagttt ttttgcaact acaatgatag ctgtaagaca
      541 agtcaaaact aagaaaaaat gaaataggca aatcctaact ttacaagtta atattacata
      601 agtaattagt ctaaaattag atactttttc tattacagaa atacatcatc cactttgtat
      661 tctcttaaaa aaataaaata gctatgtaaa ttcaagatta gatagactaa ctctctatcc
      721 atttcattgt aaaatataca tagctatttg tattcgattt aaaatactta gattttctta
      781 cataacaaat aatttctatc tgattattac tcatattcat ttagtaatca ctcagcaact
      841 aatcatttag tatctattca tccgccatac ggtaacctac cccaacctca gtcaaaatat
      901 attctggctg agcaggattc ttttcaattt tccttcttat acttgccata aaaactctaa
      961 gcgactgatt ctcactttca tagtaatttc cccaaacttt atctattata aacttatggg
     1021 ttaagactct tcctgaatac ttagacatca actcaataat cttatattcg attggagtta
     1081 aatgtatttc ttcaccatta attgacacaa tatgattttc ataatctatc tttaggtttt
     1141 taacttcaaa agactttgga gcatcattat ttatattatc ctgttgagct ttatttctaa
     1201 gagccacacg tactctagcc aaaagctctg ttacactaaa aggtttagtt aaataatcgt
     1261 ctgctcctcc atctaaagct tccaccttat ccctctcatg ctctctcgct gaaacaacaa
     1321 ttattttatt agtctttgca catgccctaa ctcgtcctat tacctcaatc ccatcaatat
     1381 ctggtagtcc taaatccaaa attatcaaat ctgggtcaag agaatgtata agtgatatag
     1441 ctgttcctcc attatctgtc tctacacacc tataattttg agtttcaaga gatactttta
     1501 taaatttaca aattggctta tcatcttcta caattagaac taatggcttt atttccactt
     1561 gtctttcccc tcctcatcat acataggtat gctaaatcta aatgtagctc ctccagaagg
     1621 attattaaag gcgacaattt ctccaccatg agcttctact atagatttac atatctccaa
     1681 tccaagacca atacctcttc tttcaagatt tctagactta ttcttagtat aaaacctatt
     1741 aaaaatattc ccgatatctt catttggtat tccttttcca ttatctgcaa cttcaaatag
     1801 tacttgattt gactcttttt ttacagttag ttctatctta gaatttttag gtgtatgcct
     1861 tatagcatta tcaattagat ttactaacac ttgttctatt aataatacat ccacatgaac
     1921 gattattatc tcatctggaa tatttgtttt tatgtctcta ctattggcaa actttttgac
     1981 tcttaatata gattcagcta ttatttcatc aacaacttct ggtcgttttt caatatccaa
     2041 ttttccttca tctattctgg tcatactaag tatattctca acagaatgag ttagccaact
     2101 cgtatcttca tatatatttt tcaataattc tactctagtt tctttatcta tcacatcatc
     2161 attttcaagt attgttgaag atgaacctaa tatacttgta agtggagttc ttaaatcatg
     2221 agaaattgac ctaagcaagt ttcctcttaa tctctctctc tctgcctcaa gatttgcttt
     2281 tttatttttc tcaaataaat tttctctatc aattgccaac gcaacctgtg ttgaaactgc
     2341 ttctaatagt atcttttcat tttctgtaat aatatctatc ttaaaacatg caacacctat
     2401 aacaccaaga acatttgatt cttgaccttt tatagggaaa taatatgcac tgctgttttt
     2461 acaatatttt gtcccaattc caactgggtt acctgtccta aaagcttctt ttatagcttc
     2521 cttttctaat actgaatgga atatattctc acttccatca ttgttacaaa tataaatact
     2581 tggcttacct agattattat tctcatctgc aatagtcact attacagttc tatttaatat
     2641 atttactaaa ttagacccac aaaattcaat aatttgattt ttattttttg ctttaagcaa
     2701 cgttttgtta ttctcataga gcaatcttat cattttttct ctcttatgag aaagtctcac
     2761 ttctcttttt atcctagaag ttaaagtact tgttataatt gctgccatta gcataataca
     2821 aaaggtcata ggataatctt ttctataagc taaaaaagta tataatggat gcgtaaagaa
     2881 aaaattaaat gatagcactc ctattactga agaaagtatt ccaaaaaaat atccataggt
     2941 tatacttgta gcaaatagta cacctaatat gtatactagt ataatattag attcattaaa
     3001 acctaccttt tcaaaaacta ctgatattcc agtagcaatt gtcataacca atatagtttt
     3061 tatgaagtcc ttaataatta tctttttact gagatagcca tcttcatcat aatcatactc
     3121 ttttaagtct tcatgttgta gtcgttcctt tttcatttac tacaattcct ccttattgtt
     3181 actgtttaaa ttggctctat tttatataat acaattttat cataataaat tcaatcaata
     3241 catttaatta atgtacttaa attactaaaa ggtgctgtct taaaatgaaa ataaatctct
     3301 atttcaaaac agcacctata tatttaaatt actaatttag tttttacata cttaaatcaa
     3361 tagttttatt caatttcata aattataata cttcaattat aaaacatttc tatccaacaa
     3421 gcataaatat aaagtgggca catatacctg cacataaacc acctatagta tgcgaaagta
     3481 tcgctttacc agccaatggt cttgcatcta aagcatccat catacctatg tgtgtactta
     3541 aatatccact ccaacacata cccattgctg tgaatactgc tatatcatta ggagttatag
     3601 cgcctgattt tataaactct ggtactaaag atattgctgc tccaacagct cctaatgcag
     3661 ttacagggaa tgctattgcc tctggtgatg tgaatccaaa taatggttct attatgaagc
     3721 taatcttatc accaatagca ggtaataatt ttataccttc atatgcagct cctgtgtaaa
     3781 cagcttgacc tgtagcaggg tctgtagatg gtccaaaagt taataacata actaatgtac
     3841 aaactactaa aactcctggt attatagcca tacccatttc aacacctaac ttaccacctt
     3901 caagtatagc atccaatgtt ctttggaata cattaccatc tcttatttct ctaaactctt
     3961 caagttttgc acctttatca tttatttgct tttcagtatc atttttaggg tcataattat
     4021 aatacttttt agtaaaagtc aacataattc taacactaat aatactacct attattgccg
     4081 caacattacc tattatagct ggtagtacat attctttacc ttgagctatc ataaaagttg
     4141 ttacaataag tcccatacca aaggctgttc ctaaattaca taaagcaggt acttgatact
     4201 tcttaaaata ttgtgtaaat gttttgtctt tagcaaatgg tataatagct ggattatcag
     4261 ataaatatgt tgcaactact cctgctatac tagcaccagg taagccccat ataggtctca
     4321 taagaccctt aaatatctta ttaactaaag atattactcc aaactctgaa agcaatgcac
     4381 taagtgcacc tgcaagaact gccatagcca ttattaaaaa tactgtgtct aataaaagcg
     4441 catgtgctgt actcattatt actttaaaca tcattcctgc acccatgatg cttcctacat
     4501 atccaaatcc taccgccaat agtacaataa atacgaaggt ttccatactt actgccttga
     4561 ctcttctctt atccgacagc ttttccatct tgaaatcttc ccccttttaa ttttaaatta
     4621 aggttcctaa cattggtaca aattttttgg tacaaactga gttctaaaac ttacactcgt
     4681 aatgcaagtt gtcagttttt tgaaataaaa aaaaattacg tttatagaat aagaatatcc
     4741 tttttaattt ggatactctt attctatttt atgttattat attatcatcc ttttgtttaa
     4801 aagtcaattt tttctatttt tgttgtttaa ttttatttat actcttttta tatttttcat
     4861 tttcttttat ctttgtaagt actccatata agttgacttt tctaaataca gtagttataa
     4921 ataccaatcc taatgcaatt gaacttgcac acgataatgt actcacccca tattctacag
     4981 cttttatata attattggtc aaaacagaat acatagtatt atatattcca taaccaggta
     5041 ctaatattat taaacagcaa acacttaatg tagttgctgg agtcatataa attcttgcac
     5101 ataactcaca atatatcgca aaacaaactg tagctataaa aaaacataag ttttctgtta
     5161 ctcccatatt taaaagaaat ttatacgaaa accaagctat acttccacct attcctgcta
     5221 gtattaagtt ttttcctttc atattaaata gcactccaaa gcttaaggca gtaaaaaaag
     5281 ctgctattac ttctataaac aaatgcactt atatcccccc taacttgaag tacaatccaa
     5341 gtaccaagaa cattcctact gctatagata cacctacaaa aaatacttct gctaattttg
     5401 caagaccaga aatcaactgt ccatctaaca aatctcttaa tgcattagtc aaagcaagcc
     5461 caggaactaa gagcattatt gctcctgcta ttaaatggtc taacgtcttt atgaatccga
     5521 gctttaaaat taaaattgaa caagaagcta taataactgc tccaattgca tttacaaaaa
     5581 agtcattaag ggaagattta cttaaataca taataacaaa cttagtcaga atcccaacag
     5641 taaatgcagc tataaattct tccacatttc ctccaaataa tatcgaaaaa gttgctgctg
     5701 caatacctgc aaaaaatatt gttattaaat ccgagtaata gttatcttca cttatttcat
     5761 ctaaaatttt ctcacataat tcaatatcta aattatttat acttattgct cttgataatg
     5821 agttaacttc atgaaccaag tttaggtcta ctcttcttga gcttattctt ttaattgctg
     5881 aatgtaattt cccatctaca aatacagatg ccattacagc tgttggagtt gcaaatacat
     5941 ctacgtggtc tattccaaaa gattgacata ttctagctat cgtttcttca actctatagg
     6001 tttctcctcc actttgcaac atagctttac ctgcatttga ggaaaagttt aaaattctat
     6061 ctatatccac aaaacatatc tcccattctt ttatagtatt attcatattt taatacaaga
     6121 tattatttat ttaaactatt ttaaataatt catatccaat atttacattt tatgaaaaaa
     6181 cttgtttttt atgcttgtca aaatcaaatt ttaagcttgt ttacaccata aaatggctga
     6241 attaatattt gacaaacact ttcaaccatt tatataacct tctaaattgt ataacttttt
     6301 actacaaata aaattatatc tcttttaatt ctttctcttg agtatttagc ttatagtaac
     6361 ttcatattta tatatgtaca atataaaaca ttatttttat gatacaaaat ataatttgta
     6421 aatgtatata tgtattaact gtgtatatat aaattattct taaccttatc agttttttca
     6481 tatatcttca tctataccga ccatatatta attacattga ccatatatta attacattga
     6541 ccatatatta ataaatgata ttacatagac ttaagtacat taaaaaactt aaatttagtt
     6601 atttaacaac ataatactct aatttaatct ataaaaatcc atacctaata taatgcaact
     6661 cttattttaa caccatctat tacacaaaac aacatattaa aaagtaaaat acaaaatatc
     6721 tactataaac cttattttaa tactgaatat atattataat atatttaatg tagcttaaat
     6781 atagtttaaa cataaagaaa ggaaacggta tatgctaaga aaagataaag ttctattatc
     6841 tctacataaa ttatgtgaaa atatgacttt aaaagacctt caatcagata aaacaggatt
     6901 cactacaata gaaattgcta accattcatc tcttgataga agtaatacca gtaaagaatt
     6961 aaataaccta tacaatgaaa aaaaagttct gaaaatatct ggtaagccaa ttttatttct
     7021 acatgctagt attatagaaa atttaattgg ccgtactttg aacagtgatg aattttctat
     7081 aaataattgt aatgagctac ttaaatcaag taaaaatgac tcagaagata ttttttcaac
     7141 attacttgga catgattcta gtcttaaact acctatagaa aaagctaaat ctgctatact
     7201 ctatcctcca aaaggtctta atacgttact cataggacct actggtgttg gaaaaagtac
     7261 atttgccgaa acaatgtaca aatatgctgt agaaagtaaa atcttttctt ctgaaagcaa
     7321 attcgtagta tttaactgtg ctgagtatgc agaaaatgct aacttattat taagtaattt
     7381 atttggttat gtcaaaggtt cttttactgg tgctaataag gataaattcg gcatagttgc
     7441 acaggctgat ggaggcatat tattcttaga tgaaatacac agattaccac cagaaggtca
     7501 agagatgctt tttcttttga tggacaaagg catatacaga cgattaggag aatctgacat
     7561 tgtgcataaa gctaatgtct ttattgtgtg tgccacaaca gaagatatac attcttctct
     7621 gcttggaact tttttaagaa gaatccctgt tacaatcaac ctacctgctt tacaggatag
     7681 gtctttaaaa gaaagattat ctcttattaa atacttcttt attatagaat caaaatacac
     7741 caatgcaact ataaaagttc acaaagatgt aattaaatca ttactccttt atgattgttt
     7801 tggtaatata ggtcagctta aatctgacat acaacttatg agtgcaagag ccttcttaaa
     7861 ttacaaaact ggacttaagg ataatataga aatagattta tcccttgttc ctgattacat
     7921 ctatattggt cttttaaaat tgaatgaaaa tcgtagtaat ctaaataaat tatcagaact
     7981 tgattatatg cttttttatg aatttaatgg taataactta gaagttgaat atacaaaaga
     8041 actatctaaa tacaatatag atacatcaaa caatggattt aaatactcta cagagattcc
     8101 ttatctggaa aatgatataa ggagtatgtt tataaattac atggaaaagt actctacaaa
     8161 attactttcg atatcttcag taactcattc tgattataca agtaatgaga tttttaaagt
     8221 aatcaatcca aagatttact atgctgttga acattcttta aaagaggctg aaaaaatatc
     8281 gagaaacaaa tacacaaaac aaacttatat tgctctttca atgcatataa gtgcacttat
     8341 agaaaatata aatcaagaca catataatcc taagtacaat ttatataatg ataaaatttt
     8401 cttagataaa gatttaaata tagctaaaat agttttaagt ataattgaaa atgacttaca
     8461 aacatcttta cctaaagatg aagtaaaatt tatatccatg tttctaaata gcatgtatct
     8521 tgaaccaaac aatcaatctg aaagtatagg agtcataata cttgctcatg gaaattcaac
     8581 agcaagcagt atgtgtgatg tagcaaattc attgcttggt acaaaccatt gtaatgcaat
     8641 tgacatgcct cttgatgtaa aagtaagtac catacttgat aaaactatag agttggtaaa
     8701 agaatgtgac gaaggtaagg gtgtacttct tttaacagat atgggctctc ttatagcttt
     8761 tggagaactt atttcagaaa aaacaaaaat caaaactcgc tcaattgaaa tggtatctac
     8821 tccaatagtt ttagaagcag taagaaaaag tacactacca gaaatgactt tggatgaatt
     8881 agctaattcc ttaagctctt taaatccata tattggacga gcagcaatcg attcaacaga
     8941 aataaactat cctgaaaagt caagagagta tgtaataata actacttgta taacaggata
     9001 tggttcagct atgaaagtag cagactttat aaattcttca cttaaaaata ttagtaaata
     9061 caatattaat ttagttcctc ataatataga aagttttaaa atgtataaga ataaatttag
     9121 taatactaaa attctggcag ttgttggaag tgtagatttt aatgtagcta acaccccttt
     9181 tatacacata gaggaactac taaatggata tggcctagat atgctaaaca aaataataga
     9241 aggtacattc aatgccaata gtattacaaa tataaaagaa acaaatctat catacaacag
     9301 tttaataata aactttatga cccaaaactt agagcttttg gactctgtta aattgttcaa
     9361 ctatgtcaat tcttccttat ttacaataat agaattaata gatgaaaact taaagagcaa
     9421 atttaagcta ggatatatac tacattgctc ttttatgata gaaagatgtt ttaaaaatac
     9481 aacttttcca tataataaca ttactgaatt aattagtaaa aatcatgaac tatatagttt
     9541 aatacgtgaa gcctttaagc caacagaagc ttattttaat attttgatac cagacgaaga
     9601 aattgcttat ataatagaca tgattaatga gtatctaaat aaataaaatt tatagcactt
     9661 attttgtgca tattaattaa tatatgaaat ggaggacgag attttgaaag gaaaaataaa
     9721 acagttaaca atacttgcat taatttttat atttataact cctgtatttg catttgcaga
     9781 tactccacca gtaccaaatt cttcaagagc agcactactt atagaccagg aaactaaaag
     9841 aatattattt gaaaaaaaca ttgatgaaaa aatgccatta gcgagtttaa gtaaaatgat
     9901 gaccttttta ttggcaatag aagcagttga taaaaatcaa gttaaagaga ctgatatggt
     9961 gaaaatagac aagtctacag cttcagtagg aggttctact tgtaaactta aagatggtga
    10021 tgaaatatct ctaggagagc ttatgcaggg tctaatgcta gtatctggaa atgatgctgc
    10081 tatagccata gctaaacata ttggtaaaac tgaaaagaat tttgtaaata tgatgaacaa
    10141 gaaagcagaa gaaataggaa tgattgatac gtattatttt aatccaaatg ggcttcctat
    10201 atatactgac ccagaacata aagaacctcc tattgaaaat atgtcaactg ctcacgatat
    10261 agttacactt ggaaagtata tgtacgacca ttatgaaaat caagttacaa gaataaccac
    10321 tatgcaagta tataatgata ctaaaaagga ttttacacat tataacacaa atcctctgct
    10381 tgtttcagta cctggtgttg atggaataaa gacaggatat acagataatg ctggatattg
    10441 ccttgcattt tctatgatgg tacctaaaga tgctaaaaac gaaagaaatc atagattgat
    10501 aggtgttgta cttggtgatg gtaacaagaa aaatagaatt tcttcttctg ctactttatt
    10561 gaaatatggt aaagataatt tccattctaa aaagatagca cataagggtg atattataga
    10621 gactccttgt gttgatggta ttgatgattt taagataaca gttaaggttg ataaagattt
    10681 atatggtgtt gtttcagata acgaaaatat caatcctaag gtagtgttta aaaatatgaa
    10741 ttatcctatc cataagggag atattgttgg tgttgcaaag tattataatg actctggtaa
    10801 atttgttgga agtgttgatg taaaaagtga aagtaatata ggatgtattc ctttgaagga
    10861 taagattaag attaaggttg ctaagataaa taaaaaactt gagattaaaa attctgtttg
    10921 ttttaaagca tagtaaagca tattattaaa agggtatgtt aaatttataa agactccact
    10981 ttgtggagtc tttttgtttt ttacaaaaaa ttaaaatagt ggcaacaatt gaggatgtga
    11041 gggagagttg ccactatttt gtatagggta ttatattatg tttttaagct agttttacta
    11101 tcttctatgt ttatataata actcttgtgt gtgaatttaa ttgataatta atatgaaaat
    11161 tatgtgaagt tttatgtgaa tcttattata agcatactaa aagagctgta ggtaagttta
    11221 cttttatgca aatttaatta cagctccttt cattatatat taattttatt tactaaaatt
    11281 aaaaatattt actgctattc tacctaatag agatgttggt gttgatactg tctttgctgg
    11341 tactgttaca tctatctctg ctgtatctgc tgtttttgaa cttgctgtat gtgtcactgt
    11401 aaatactagt tttactgttt tttctgcatc tggtggcact attgttccat ctgtctttat
    11461 tacat
//
LOCUS       DACXMZ010000121        11367 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.122, whole genome shotgun sequence.
ACCESSION   DACXMZ010000121 DACXMZ010000000
VERSION     DACXMZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11367
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.122"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            199..1032
                     /locus_tag="JD016_12275"
     CDS             199..1032
                     /locus_tag="JD016_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HAU5000410.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1101..2057
                     /locus_tag="JD016_12280"
     CDS             1101..2057
                     /locus_tag="JD016_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAU5000411.1"
                     /translation="MNKKLYIGMVGMLSLMMVGCNKSLAKVNDVEITKEQYKKTKAVL
                     SATNNYINGQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVD
                     SKYQELEDKMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDM
                     EAYYTSHKKDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESF
                     ESLAKKYSDDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVK
                     VTDKRERQKSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2109..2591
                     /locus_tag="JD016_12285"
     CDS             2109..2591
                     /locus_tag="JD016_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAU5000412.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2603..3472
                     /locus_tag="JD016_12290"
     CDS             2603..3472
                     /locus_tag="JD016_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-V factor rsiV"
                     /protein_id="HAU5000413.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQDRMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3515..4270
                     /locus_tag="JD016_12295"
     CDS             3515..4270
                     /locus_tag="JD016_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAU5000414.1"
                     /translation="MTTYKKLKKSIFKIAIFLICLLVSLVDINPIFAGSSSNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRSISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4312..5133
                     /locus_tag="JD016_12300"
     CDS             4312..5133
                     /locus_tag="JD016_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HAU5000415.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5138..5899
                     /locus_tag="JD016_12305"
     CDS             5138..5899
                     /locus_tag="JD016_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAU5000416.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIVEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6060..6317
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6430..6669
                     /locus_tag="JD016_12310"
     CDS             6430..6669
                     /locus_tag="JD016_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000417.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            6973..7968
                     /gene="ilvC"
                     /locus_tag="JD016_12315"
     CDS             6973..7968
                     /gene="ilvC"
                     /locus_tag="JD016_12315"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAU5000418.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VMIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8015..9709
                     /gene="ilvB"
                     /locus_tag="JD016_12320"
     CDS             8015..9709
                     /gene="ilvB"
                     /locus_tag="JD016_12320"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HAU5000419.1"
                     /translation="MCMNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFK
                     HIRTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQ
                     VPTSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVD
                     VPKDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVI
                     YAGGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLA
                     LSNSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLV
                     LSLLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVT
                     DVGQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSF
                     RMNCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVN
                     AYNIDGYKVASMEELGKALDVIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(10070..11200)
                     /locus_tag="JD016_12325"
     CDS             complement(10070..11200)
                     /locus_tag="JD016_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAU5000420.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLNNKTITVRHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
BASE COUNT         4608 a         1156 c         1980 g         3623 t
ORIGIN      
        1 atataatact caattttcaa cattctatac aattatacag aaattataca gcataataat
       61 caccaaaata acaaatttta aaaattaagc aaaacttttt gtcgaagttc aacgtctata
      121 tatatgtagt gtaaattttt gggataaaat actacaaaaa tataaatgta aaaatatagt
      181 aatattggag gtcgaacgat gagaaataaa aagaaaaaaa taaatagaaa gaagttgtat
      241 ttgcttttaa gtgctattgt agtgtgtctg gcttttatag tagttggtgt tcgcgtcagc
      301 tctttaaata tgaaggaaaa tgaaattagt agaaatgcaa aactgtcaag ttctactatt
      361 acagatatag tatctaatac atcaatagaa gttagtaaag ggccaagtaa aagttctgga
      421 aaaatagcat acataactat agatgatggt ccatctaaat ttactgacga gatgataaaa
      481 actttaaata aatacaatgt taaagcaact ttttttatga tagatgggaa tatgaaggaa
      541 tatccacagc aagttaaaaa tataattaaa aatgggaaca cagcaggttt tcatagtgta
      601 tcacatgata ttcacaagct ttatgttact agtacatcag caaaagagga atttgataca
      661 aatgatcaaa ctttttataa gataactggt aaacattcaa aagtaataag aataccttat
      721 ggaagcaaac catatacacc acaagcctct tatcaggctt tagttgatgc tggatataaa
      781 atttgggact gggacttaga tacagaagac tggagatcta attcttctca aattgtacaa
      841 aatgtaaaaa atcatattaa gaatagaaaa ggtgaagata aagaccaact tgtagtttta
      901 atgcatgaga aaaaacagag tgcagaagct ttagattcag ttttaaaatt cttatcagat
      961 gaaggttatg aatttgcgcc agtagatcaa aaccaaatac caaaaaatta ttggttacgt
     1021 aatttagagt agttaaaata taagaatatt gattgatatt tttagaaatt gagaataaaa
     1081 atgagtgagg tgtaggtata ttgaataaaa aactttatat aggaatggtc ggaatgttgt
     1141 ctttgatgat ggttggttgt aataaaagtt tagcaaaagt aaatgatgtg gaaatcacaa
     1201 aagaacagta caaaaaaaca aaagcagttt tatctgctac taataattat ataaatgggc
     1261 agtctttaga tgaattagaa aaaactttgg ataaaaaagg tagaaataaa ttagaaaatg
     1321 ttataatatc gtttatggtg gataatgaac ttttatatca agaggctaag gataaaggac
     1381 taactccaag taaaagtgaa gtagactcaa aatatcaaga actagaagat aagatgaatt
     1441 tgaatacaag ctataaggaa aaaatggaca aagcaggtgt tgataaagat tatttaaaac
     1501 aagaaatatc tagagattta gcaatagata aaaataaaaa ggcctttgaa gatagaataa
     1561 acataagtga taatgatatg gaagcctact atacaagtca taaaaaagat tttaatgtag
     1621 aagaagtaag tgcttctcag atacttatat cgactttaga taaaaataaa aaagaagtaa
     1681 gcaaagataa aaaagaagct ttgaagaaga aggcagataa tatattgact aaaatcaaaa
     1741 atggagaaag ttttgaaagt ttggctaaaa aatattcgga tgataaagca actggaaaaa
     1801 atggaggaca gctaggatat tttacaaagg atgataaaaa tgctgagttt acaaaggaag
     1861 tgttcaaatt aaagaaaaat gaagtttcaa atgtgtttga aactagttat ggatatcata
     1921 tagtcaaagt tactgataaa agagaaagac aaaaaagttt taatgagtgt cagagtttaa
     1981 taagagaatc tattttaaat gaaaagtata ttgagcatat aaaaaaactg aatgaagatg
     2041 ctaaaataga cagatagaat ttctaacagc tcaatcctag cctgtaatcg gagggagatt
     2101 ttacaggaat gtttaagagt ttaaagagaa cgaaagttga gaaatacatt atcgataata
     2161 aagactcttt ttatagaata gcatatagtt atacgaagaa tgaagaagat gccttagatg
     2221 tggttcaaga agccatgtat aaggcactat actcagtgga aaatattaaa gaagtaaatt
     2281 atataaagac atggttttat aaaattttag ttagaacgag tatagatttt attagaaaaa
     2341 accgtaaata taataatatg acagatattg accttataga tgagactgga gagtatgata
     2401 aatatactga tttggattta agaagagctt tggaagaatt acctattgaa tacaaatcta
     2461 ttataatatt aagatttttt gaagatttaa agattgaaga agtagcaata atattggatg
     2521 aaaatgtgaa tactgttaaa actaggttat atactgcttt aaaaaaacta aaattaaaaa
     2581 ttgaagagta gggataaaaa gaatgacaag caaagaaagg ttaaataagt taaaagatga
     2641 gtatcataaa attccaatac ccaagaaatt agatactata ataaatcatg aaaaaataga
     2701 aaacatatat cgagagaaaa ataaaaaagt aaatagatta agatttaaag ttgcaatagc
     2761 ctttgcatgt atatttacag tattagtaaa tataagtcct gtatttgcag acaatttttc
     2821 taagatacct gtaattggag caatagtaga agtaataact ataaaaaatt atagtttaaa
     2881 aagtgaaaat tatgaagctg agattgatat tcctaaaata cgaggtttaa aagataaaaa
     2941 cttagaacaa agattaaaca gtagttttat ggaagatgga aaaaggcttt atcatcaatt
     3001 ccaagataga atggagaaga tacaatcatc aaagaataaa ggatataaat ctttaagttt
     3061 gagttatagt gtaaaaaata attctaaaaa atttctatca atagagatga caaaaaatga
     3121 gatagaggct tcatcatatg tgagtaaggt acattatact attgataaaa aaagacaaat
     3181 agttttgact ttgccaatgc tttttaaaga tgataagtat ataaaagtta taagtgataa
     3241 tataaaagaa caaatgagag aacaaatgaa aaaggatagt acaaaatcct attttataga
     3301 tcaaaaaaaa gacttgcctg ttgaagattt taaaacaatt aataagtatc aggattttta
     3361 ttttaataaa aatgaagacc ttgtaatttg ttttgatgaa tatgaagtag cacctggtta
     3421 tatgggcgca gttgagtttg taataccata taaagtaata aaagatttgt aaattgaata
     3481 gtagtttata tataaatctt ttaaggagga caatatgact acatataaaa agcttaaaaa
     3541 atctatattt aaaattgcta tatttttaat atgcttatta gtttcattag tagatataaa
     3601 ccctatattt gcaggtagtt cctctaatga gacttcaaat agtgatacga taaaggtgtt
     3661 aaatgctaag agtatcaact ttaaacaaga caattatgaa gtgaatgtga gtattcctaa
     3721 aataaaaggt ttaaaagata aaactttaga gaataaatta aacaatgagt ttttgcaaga
     3781 tgggaaaaaa atttacagtg aatttcaaaa taaaatgaaa aaggaaaata ataagggtca
     3841 tagataccta aatgtaatcc ataatgtcaa aaataattct aaggactttt tatcaataga
     3901 aatgacaaaa gaagagatag aagcttcctc atctataact aagaagcatt acactgttga
     3961 taaaaataga ggaatactat taaatttacc aatatttttt aaagatagta gatatattag
     4021 atctataagt gataatataa aagagcagat gagaaatcaa attaagaaag atgatacaaa
     4081 atcttatttt atagaccaag ataaagactc cccagtgaaa gattttgaat ctataaataa
     4141 gtatcaagat ttttatttta ataaaaatgg aaatcttgtg attagttttg atgaatatga
     4201 agtagcacct ggttatatgg gagcagtaga atttgtgata ccaaataaag taataaaaag
     4261 actaagatag atatatagaa gatttttaga tagactggta aggagaatta tatgaatata
     4321 tatgaacaag caataagatt aatagaaaat aatgaggatt tcgcatttgc tacaataacg
     4381 tctcattcag gttcaacacc acgtgaaact ggagcaatga tgattgtgaa aaatgattct
     4441 actatatttg gctcagtagg aggaggtagt gtagaagcag aatgtataaa acatgcaatt
     4501 aatgttataa aaaacagaga atcaatgttg tataaattta ctctaaacaa atctgatgtt
     4561 gcaaaactag gaatgatatg tggaggtact ggagaaatac aaatagattt tatagatagt
     4621 aaattaaaaa gtaatataga aaaatttaat aagagactaa aggaaaatac aagtaaagca
     4681 tatatatttg gtgcaggtca tatttcaaga gatgttgctg tgatactatc tcttttagaa
     4741 tttagaacag tagtaataga tgatagagaa gaatttgcta atcatgaaag gtttccagaa
     4801 tcagaagtag tagttttaga ttcttttgaa aacataccag attttccaac tgacgaaaat
     4861 agttatataa ttattttaac aagaggtcat ctatatgatt caagtgcttt agaatgggct
     4921 cttaaaagag aggctggtta tataggtatg attggaagta gaacaaaaat tggattgact
     4981 tacgaaaaac tcatgaaaaa aggttttaag aaggaagaat tatcaaaagt acatgcacca
     5041 ataggcataa aattgaatgc tcaaacacca gctgaaatag ctgtatgtat agctgctgaa
     5101 cttataaatt gtagagctaa taaggaaaaa taaactaatg aacacaggga gtaaagatat
     5161 tgatacttta aaggttaatt ttagagggat tgaaataaaa gcagttttag tatataaaaa
     5221 tagaaaaaat atttctataa aaatagatcc atttggcaaa ataactgtaa tgagtcctcc
     5281 taaaatatcc aaaaaaataa taaaagatat aattgttgaa aaaggagact ggatacttaa
     5341 aaagtcagaa aaatataaag gaagagagga agtatataaa caaagaatgt ttataactgg
     5401 cgaaaaattt ctttatttag gtgaagagta ccatctagtt ataaaagaac tgttgagaga
     5461 ttctgtcaaa aaaagtaact gtagaataac tattaacgaa tatcaaatag ttgttcagac
     5521 aaatgataca agtacagatt ttattaaaaa atctttaaaa tcttggtata agatggaaag
     5581 tgaaagaata gtattagaga gaatagattt tcttaaatta aattgtgaaa taatgaagca
     5641 attagttcca gcgagtgtta aggtaaaaga acaaaataag agatggggaa gttgtacagc
     5701 tcaaaaaaat atttatataa actcaaaaat atctatggca agattagatg taattgatta
     5761 tgtaataata catgagttta gccatttagt acatatgaat cactctaaaa aattctatga
     5821 tttagtaaaa tccattatgc ccgattataa agataaagaa aattggctaa aaataaatgg
     5881 atataaaata ataatctgaa aacgattatt ccaaaatata ggcgtaaagt ataaaagaag
     5941 tattattgtg aaaaaaataa ataatctgaa aattttaaaa aaagtcattg acattgtaaa
     6001 tgatagggaa tataatctat attaacaaca aaacagaatt gaaatataaa attaaaaaat
     6061 taaaagcaat gacagagaca acaatactaa tagtgttatc cacagagaac caatgttgct
     6121 gagaattggt gttaacagta gtataatgat cactctggag cttttgtact gaatattttt
     6181 agtaagtaca aacgtatgtc aacgttacat gactaaggtt aaggtaatta attacccgac
     6241 ctgaaaaaag agaggtagat gtatttttta tatctacaaa taaggtggta ccgcggaata
     6301 taactttcgt ccttataagt aatatttata ttgcttataa agatgaaagt ttttttattt
     6361 taagcgatat aaataaattt aatccatata aaattaataa aaaaataata aaaataagga
     6421 gggattttta tggaaaatac tgtatatgct aaattaaacc aaggaataga ttctttttta
     6481 agagtggcta tgactctaag aagaagagag gttgatatac aatcaataag tatgactgta
     6541 gataatatga ataattcagg tataaaatta atagtaaatg aagaaaagac atctttagat
     6601 tctgtcttaa accatatgaa aaaactacat gatataagag agataactgt tgaaagaatg
     6661 agacattaat taaatatatt ttataaagct aaagataaat atgcttattt taaaataaaa
     6721 aactaaagat aaacattaaa taagaaaaat cttagattaa aatataagtg tcattgaatt
     6781 tattagttta tcacagttaa taaatttatt agtttgttgt agctaataaa aactgaaaat
     6841 tctaaaatta taattgtaag attatataaa tttagaattt aaatctgttg aataatatta
     6901 aaacaatgat aatgagtatg aatttctaat aaaaataaat atcataaaaa taagaaaaag
     6961 tattgggagg aaatgaaaat ggcaagaatg tattatgaaa aagatgtgga tttagaggtt
     7021 ttaaaaaata aaaaggttgc agttttaggt tatggaagtc aaggacatgc acatgcacaa
     7081 aatcttagag ataacggtgt acatgtaatg ataggtcttt atgacggaag taaatcagca
     7141 caaaaagcaa aagaagatgg atttgaagtt aagagtgtag cagaagctac aaaagaaagt
     7201 gatttgacaa tgatgttaat gcctgatgaa aaacaaaaga aagtttatga agagagtgta
     7261 aaggataact taaaagaagg tcagacatta gcttttgctc atggattcaa tatacattat
     7321 aatcaagtac aaccaccaga atttgttgat gttgtaatgg tagctcctaa aggacctggt
     7381 cacttagtta gaaatgtatt tacaaaggga agtggagttc ctgcactatt tgcagtatat
     7441 caagactaca ctaaaaaagc tacagagaca gttttagcat atgcaaaagg gataggagca
     7501 acaagagcag gtgttttaga aactactttt aaagaagaaa cagaaacaga tttatttgga
     7561 gaacaatcag tactttgtgg tggaataagt gaacttataa aattgggata taaaacgctt
     7621 gtagatgcag gttatcaaaa agaagtagca tattttgaat gtttacatga aatgaaacta
     7681 atagtcgacc ttatatatga aggtggattt gaaagaatga gatatagtat aagtgacaca
     7741 gcagaatatg gagattatgt atctggtaaa agggtaataa ctgatgctgc aaaacaaggt
     7801 atgcaaaatg ttttagagga tatacaaaat ggaaaatttg caaaagcgtg gattaaagaa
     7861 aatgaagaag gacgtgaaaa cttcctaaaa actagagaag aagaatataa tacagaaatt
     7921 gccgaagttg gtagaaatct aagaagtatg atgtcattct taaaataaat atatattaaa
     7981 tggattagtt tgagggaaag agtggtgtat agtagtgtgt atgaatggag caaaggtaat
     8041 tttggaatgt ttaaagaaag agggaattga cacaatattt ggatatcctg gtggagccgt
     8101 gatacctctt tatgatgctc tatacgatta ttcagatgat tttaaacata taaggacatc
     8161 ccatgaacaa ggtttagttc atgctgcaga tgggtacgca agaagtacta atacagtagg
     8221 agtttgtttt acaacatctg gtccaggagc aacaaatgcc ataactggaa tagcaacagc
     8281 ttttatggat tcttcaccta tggttgtaat ttcagggcag gtgccaacta gtttattagg
     8341 aaaagattct tttcaagaaa ttgacataac tggagcaaca ttatctatga caaaacataa
     8401 ttatttagtt agaaacacta aagaattagt tcctacaata aaagaagctt ttagagttgc
     8461 aaattcaggt agaaaaggac ctgtacttgt agatgttcca aaagatttat ttttagcaga
     8521 aatggatttt agtggtgagg actatgattt atgtcaaata gatgattata tggattataa
     8581 gagtgatttt gatctagatg atgaaactaa tattaagctt ttaaatgaag caatagatat
     8641 cataaaagaa tctaaaaagc ctgtaatata tgcaggtgga ggtgttaaat cttcagacag
     8701 tgaggaaatt cttgaaaaat ttgcaactaa gatagataca cccgtactaa atacacttat
     8761 ggggcttgga aatatagata gaaagaatga actatctctt ggaatggttg gaatgcatgg
     8821 aagtagagaa agtaatttag cactttctaa ttcagatcta gtgatagcga taggtgcgag
     8881 atttagtgat agagtgataa gtaaaagttc tgaatttgct aaaaatgcaa aaataataca
     8941 catagacata gacccatctg aaataagtaa aaatatagaa tctaatgtat ctttggttgg
     9001 agatgtaaag ctagtcctta gtttattaat tgaaagggtt gaaagtaaga ataatagcaa
     9061 ttggaaagaa gagataaaga catttagaaa gagtgagggt gtacaaacat atgaatttca
     9121 tccacaaaat atacttaaaa aaataaatga aaaatatgaa acattaaaga aacccactgt
     9181 ggtagtaact gatgtcggtc aacatcaaat gtgggctgct aagtactgga attttaaagg
     9241 taataaaagt tttataacat cagcagggtt aggaactatg ggatttggtt taggtgcagc
     9301 tattggaact aaagttggta atgtagataa aaatgtagtt ttagtcactg gtgatggaag
     9361 ttttagaatg aactgtaatg aactagctac agttgcaaat tataatgtac ctatgcttat
     9421 tttactactc aataatagaa cattaggaat ggtaaggcaa tggcaaaagt tattttccaa
     9481 tcaaagatac tctcaaactg acattaatga aaatgtggat tatgttaaac tcgtaaatgc
     9541 atataatatt gatggatata aggtagccag tatggaagaa ttaggaaagg cgctagatgt
     9601 gatagatttt aataaacctg tatttttaca atgtgatata gataaggatt atgatgttta
     9661 tcctattgta gcacctaatg atgcattgga aaatttgata tgtaactaaa acttatactg
     9721 aatattgata aaatttttta ttaaattttt attggtattc agtttttgtt gtttagagat
     9781 agattggcat tttttatttt tgcaaataaa tcaataacta tataattaag aacttattaa
     9841 attttaatta tacaatttag aatagtatta caacttaagt aaatattaag ttttatttgt
     9901 tagatttatt attaatacct tatattagtt atggttgtta tatactctaa agccttgaaa
     9961 ttacatgcgt atagctgact atataaattt gataatttta gcttttaaaa tagataaatt
    10021 agattttgcc acccatttgc caccgtatta tatttttggg tggcaaactc taatttgtaa
    10081 ttttttcaaa tatatctaca gtttcatttt tcattttatc agttacatgt gaataggtat
    10141 ccattgtagt tgatagttgg ctatgaccca aacggttttg tatgtcttta atgttagcac
    10201 cattttctaa taatagagta gcatgtgcat gtcttaaaga atgaaaatgg aagtcattgt
    10261 ttaaagctac tcgaatttgt cttactatag tgtctaaagt gtgagtattc acttgttgac
    10321 cattttcttt ggtacatacc cagtcactat caaagtaaaa ttctccatat tttaatttca
    10381 ttttcttttg atataattta tgttctttta atgcctttat taaagtgtca cctgtaaata
    10441 tagttctgca agagctttct gtttttggtt gccctaattc aaacattcca tttggctttt
    10501 taatcagagt atgtcttact gtgatagttt tattattaag gtctatatta tcccatttta
    10561 gtgcaataat ttcacctctt cgcataccag tatgaaatcc aattagtaaa actatacgtt
    10621 gaaatgaatc ttgaggaaat atatttagta tttgattaaa ttcttctaat gtaatagttt
    10681 taactttatt agtttctgtt ttagatttag tttttggtat gcttacatat tgcatagggt
    10741 tttctcgtat gtgtttgtaa ggatggactg ctgattttaa tgacctatgt aatatggctt
    10801 ttaatacttg taatgtattt tgagagtaat cctctttgta ctttttattt atgaagtttt
    10861 gtagtattgc aggagttaaa gcttttactt tgtaagctcc tagttttggc tttatatgtt
    10921 tttctatgtt tattcggtag ctttcttgag tgttgtattt acagttaagt aagacatatt
    10981 ctttgtacca aaaatctaag tagtctgata aactgatgtt gctttcttca aatactatgc
    11041 cagagttttc atattcattt agtgcttctc ttaaggcttt ttcggcttct tttttagtat
    11101 tgcctccaac tctttctact ttttttcttt ttccttctac tatgcctagg tcaaagtagt
    11161 aataccattt gttacttctt tttcttactc cacctttcat aatagtatcc ctccctttta
    11221 gaatgtatgt ttgtttggtg tttatataaa agagcagatt aactgctctt tatatacttt
    11281 tgttatgtac aatattatct taattttgtt tatctggttt ttacttaatt attttcttaa
    11341 tattcttaca attttttagc ttttatg
//
LOCUS       DACXMZ010000122        11336 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.123, whole genome shotgun sequence.
ACCESSION   DACXMZ010000122 DACXMZ010000000
VERSION     DACXMZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.123"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            170..1183
                     /locus_tag="JD016_12330"
     CDS             170..1183
                     /locus_tag="JD016_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAU5000421.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            1225..2115
                     /gene="rbsK"
                     /locus_tag="JD016_12335"
     CDS             1225..2115
                     /gene="rbsK"
                     /locus_tag="JD016_12335"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAU5000422.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            2163..3125
                     /locus_tag="JD016_12340"
     CDS             2163..3125
                     /locus_tag="JD016_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAU5000423.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            3179..4690
                     /locus_tag="JD016_12345"
     CDS             3179..4690
                     /locus_tag="JD016_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAU5000424.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            4674..5672
                     /locus_tag="JD016_12350"
     CDS             4674..5672
                     /locus_tag="JD016_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAU5000425.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            5856..7118
                     /locus_tag="JD016_12355"
     CDS             5856..7118
                     /locus_tag="JD016_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAU5000426.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            7138..7881
                     /locus_tag="JD016_12360"
     CDS             7138..7881
                     /locus_tag="JD016_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAU5000427.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IESEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            8054..9439
                     /locus_tag="JD016_12365"
     CDS             8054..9439
                     /locus_tag="JD016_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAU5000428.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTIKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDSCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            9658..10497
                     /locus_tag="JD016_12370"
     CDS             9658..10497
                     /locus_tag="JD016_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000429.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            10487..10930
                     /locus_tag="JD016_12375"
     CDS             10487..10930
                     /locus_tag="JD016_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000430.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            10932..>11336
                     /locus_tag="JD016_12380"
     CDS             10932..>11336
                     /locus_tag="JD016_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000431.1"
                     /translation="MINMSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVS
                     IVIMEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIE
                     YFMKLVFANIMILILYFLLRTIVYIPINIFIIG"
BASE COUNT         4560 a         1122 c         2190 g         3464 t
ORIGIN      
        1 tttttgcaga ttaatttaaa tattaaaatt gaattataag ttgcaatatt ttgaaaaaag
       61 ggaaaaatat caaaatttat ataaaatggt tgcaaatgga aaaaagatgt gataatatct
      121 aggtaaccgg ttaagtaaaa cggtttccta aattttgggg gtgaacttct tgagtataac
      181 tataaaagaa attggcgaat tggctggtgt ttctaaaaca actgtgtcaa aagtcattaa
      241 taataaagat gaaaatataa gtcaggcaac aagagaaaaa attttgaaaa taatgaagga
      301 aaaaaattat gtaccaaata aattagcaca aagtttagta actaaaaaaa ctaatactat
      361 agggcttctt ataccagata tacgaaatcc attctttaca gatgtatcaa gaggtgtaga
      421 agataaagct aatgaagaag gctacaatat aattttatgt aatacagatg aagatgctaa
      481 aaaagaatat gaaggaataa gaacactatc agagaggatg atagatggaa ttatatttgc
      541 agcatcttca aatacaaact ggaaagaagc aaattataaa gatataaaaa taccaactgt
      601 cttgattgat aagaagataa gtatgaacaa agaaacctta aaaggaattg ttaaaataaa
      661 taactttgaa ggggcctata tagctacaaa acatttatta gatattggaa ataagaaaat
      721 aatataccta agtggtccac ttcaaaatga aatagcagta gatagattag aaggatataa
      781 aaaggctcta atcgagtgca atttaagcta caatcctgct tatgtatttg aaggaaaata
      841 taagattgaa tggggacaag aatttataaa aaatttagaa aagatagatt ttgatgcaat
      901 attttgtgca aatgatttga ttgcaatagg tgtcataaga ggtcttaaag aaagaggatt
      961 aagtatacca aatgatataa gtgtcgtggg atttgatgat attcaaacat caagtctaat
     1021 aagcccttct cttaccacag taaaacagcc gtcttatgat ataggatata aagccagtga
     1081 aatattgata aattgtttaa gaggagataa aaaagaatcg tttgatgaat tgattttcaa
     1141 accagaattg gtcataagag attcaacaaa agaaagtgaa taacagcacg agagacataa
     1201 atagatatgt aaaggagtaa tgtaatggga aacatagttg tcataggaag tgtaaacatg
     1261 gacatggtat gttctgtaga taaaagacca gaaaaaggag aaacagtatt aggtaatagt
     1321 ttttttacat cacctggagg aaaaggggct aatcaagcta tctcagcttc taaactagga
     1381 gcaaatgtaa aaatgatatc atgcataggg gaagatggct taggagagga gttaataaga
     1441 aattttagaa cgaataaagt tgattatagc ttagtatcca gaaataatca caaaagttct
     1501 ggtgttgctg ttataacatt atgtgaaaat gataatagta ttgttgttgt accagggact
     1561 aatgagctag tagatataga attaattaaa aagaatgaag aagagataaa gaatgcagat
     1621 atagtattgc tacaattaga gattccatta aaaacaataa attatgtagt gaatttctgt
     1681 tttgaaaata ggattagggt tttattaaat ccagcaccag cagtaaaact aaatgaagat
     1741 ataatagaaa aagtaactta cttaacacca aatgaacatg aatataagat agtttttgac
     1801 acaaatgaag ggatagaaga agtattaaaa aaatatccaa ataaacttgt aataacagaa
     1861 ggaaaaaatg gagctagatt ttatgatggt gaggaaatca agcatgtatc ttgtataagt
     1921 gttgatgttc aagataccac aggagcagga gatacattta atggagcatt ggcagtggct
     1981 ataacagaag ggaaaaattt atatacagca gtagaatatg cagtagtagt atcaggtctt
     2041 tctgtaacta aactaggtgc acaatctggt atgccctata aagaagatgt tgaaaagtat
     2101 ttaaataata aataaaaatc taatttgttc aaatttaaaa ctattaaaaa tggaggttaa
     2161 ttatgagagt gagaaaaatt ttatctttag gaattgctgg aatactagca ataggaatgt
     2221 taacagggtg ttcaatggaa ggaccaagta aatcagataa taaaggtggt tcagacaaga
     2281 aagatttaac tattggagta tctacaataa ctcttcaaca tcaatttttt atagatattg
     2341 atgagggaat aaaagaaaaa gctaaagaat taggtgtgaa agttatagta aatgacccag
     2401 accaagatgt agctaagcaa acatcagcaa tagaagattt tatacaacag aatgtagatg
     2461 gaatgatagt acttggaact gataattcgg ctatagtacc agcagtagaa ggagcatttg
     2521 aaaaaatgcc agtagttaca gtagatgctg tattaaatac tgaaaatatt acaagttatg
     2581 taggaacagt tagttatgat gctggtaaaa aattagggga atacactaaa aaatacatag
     2641 atgaaaagtt aggtggaaag tcagaaatag caatagtgac agatttaaaa tctcaaatac
     2701 aaatgcagag aatagatgga tttaaagatg cattaaaagg ttcagcaaat gttaagatat
     2761 taaattcaca accaggatat gacagagaag aatctcttaa tacagtagaa aacttaattc
     2821 aatctaatcc agatgtagat ataatatatg caacagctga aaatagtgta ctaggagcta
     2881 aagctgcact tgaatctgca aaaaataagg atgttaagat tgtaggattt gacttaactg
     2941 aagaagcatc aagtggaata acagatggaa ctattcttgc tatgattcaa caacaaccta
     3001 aagagatggg acgtttagct gtagaagcag tagtaaaagc tataaaaggg gaaaaggtag
     3061 aaaagaatat accagtacca gctttacttt atgataagga aaatataaaa gactttaaga
     3121 attagtaaaa taaaatgaga ttatttgaaa gaaatcctat ttaaaaagga gtgtcattat
     3181 gagtaatata attctgaagt taagtaatat tgctaaagaa tttcctggtg tcagagcatt
     3241 agataatgtt aattttgagt tatttcatgg cgaagttcat gcactactgg gtgaaaatgg
     3301 tgctggtaaa tcaacaatga taaagatttt aactggtgca cattcgaaaa cttctggaaa
     3361 atttattttt gaaggcaaag aaatagagaa tatatcccca gatatctcta aaaagatagg
     3421 tataaatgcc atatatcaag aactaacagt ttttgacgaa ttaacagtag cacaaaatat
     3481 ttttatggga aaagaaataa atggcaaagt cttaaccaat gacaaaaaaa tgaatgaaga
     3541 ggctaaaaag attttcgata atatgggaat agatataaat ccaaatagtt tagtcaaaga
     3601 gcttagtata gctcaaaaac aaatggtaga aatagcaaga gttttatcat cagaaactaa
     3661 agtattaata atggatgaac ctacttcttc aataagtaaa aaagagactg aaatactttt
     3721 tagacttata aatgatttaa aagagagtgg agttagtata atatatatat cccataggat
     3781 ggaagaactc tttgagattt gtgacagaat aactataatg cgagatggga aaacgatttc
     3841 gactttaaat accaaagatg tatcgtctga ggaagaatta gttaatttaa tgatagatag
     3901 aaagctagac caatttttcc caaaaagaaa agtagaaata aaagaagaaa ttatgcgagt
     3961 ggaaaatcta actaagaaca atgtttttaa tgatattagc tttaatataa gaaaaggtga
     4021 aatattggga ataggtggac tagttggaag taagagaagt gaaatagtag aagctatatt
     4081 tggacttaga acatatgatt ctggaaaaat atatttaaac aatgaagaag taaagtttaa
     4141 aactccatca gatgcaatag aaaatggact aggattaata acagaagata gaaaaggtac
     4201 tggattgttt ttacaaatga gtgtcaaaga aaatacaact atggctgggt taaaaaagat
     4261 atctaagttt aaatcaatta tagatagaaa aaaggaaaaa gagattttag aaaaatacat
     4321 agaagcttta aagataaaaa caccacatat gaatcaagtt atacaaagtt taagtggtgg
     4381 aaaccaacaa aaagctataa tagcaagatg gcttttatta caaccagata tattgattat
     4441 ggatgaacca accagaggta tagatgtaaa tgctaaggca gagatataca atctaatggg
     4501 agatttagtg gagagtggtg taagtataat aatgatatct tctgaaatac cagaattaat
     4561 atcaatgagt gacagaataa tggttatgag agaaggtcat ataagtggat ttttagaagg
     4621 tgaagaaatg gttgaaaata atgtattaaa attggctttc ggaggtaaaa ttaatgagtt
     4681 caataactaa gcataaagaa gaaaaatctt ctccattatt gacaaaatta acaagtgtat
     4741 ttagagacca agggagtgca gctgtaggac ttgtaataat ttttataata atgagtgtag
     4801 cttcatctaa ctttttaact ttagataatt taattaatgt tggaagacaa atatcaataa
     4861 atgcaatact tgcagtaggt atgacatttg taataattac aggaggaata gatttaagtg
     4921 taggagcagt aattgcttta gtaggtacat tttgggctac aacagtagtg aattataatg
     4981 cacctatatg ggtaggtatg atacttgcat tagcaatagg gacaatatta ggagtcataa
     5041 aaggtgcaat tatttcaaca caaaaactac ctccattcat tgtaacttta gccatgttaa
     5101 cgataataag tggggcaagc tttgtattta caggaggaag acctatatca gtaaatacag
     5161 atgcatttaa gatgctcggt agaggatata ttggaccaat accaatacca gttataatta
     5221 tgattatagt tgtaatagca ggacattttc tattaaaaag aactaatttt ggacgtcatg
     5281 tacatgcagt aggtggaaat gaagaggccg ctagattatg tggagtaaaa gtaaataaag
     5341 ttatagtaaa agtatacgca ctagctggac tactaacagc attagctggg ataatactat
     5401 catcacgttt ggcatcaggt tcaccaactg ttggagatgg agcagaattg gatgctatag
     5461 cagccgttgt gcttggtggt actaatatga tgggtggttc tggttctata gtaggtacat
     5521 gtataggtgt cggaataata ggaatattag gaaatggatt aaacttatta agtgtatctt
     5581 catataatca aatgattata aaaggtttgg taatgttatt tgctatatgg ataaataata
     5641 taaaattaaa aaaatcagct aaatctaaat agtaattaaa ttttagctta taaactataa
     5701 aaattattaa gtttttgtat atatttttat actttaattt gcatacatct ttatacattg
     5761 aatgagaata tgctaagtga aaaagtttac tatggcttaa atagatgaga ttagacttga
     5821 aattttaaat gtaaaaataa atcttaggag gaaatatgaa aaaaattctc ttagatacat
     5881 taaatagtaa aaaacaagaa tacatagatt atttaaaaga acttgtaagt ataaagactg
     5941 aagatgttgg tcatggaata ctaggtggat ttgaaaaaga aggacaggaa tatattgaaa
     6001 aacttgcaaa ttacataggc tttagtgtag atagacaaga aatgagcgaa gaattaatta
     6061 aaaaagcaaa aaatatatat aaagaaggaa atctaggtca caattatcaa gatagatata
     6121 atttaatttg taagtatagt gatgatttac ctggtaagac aattgtattt aatggtcatg
     6181 tagacactat gccaccgggt gatataagta agtggaaata taatccatat cgtgctacag
     6241 aagataatgg aaaactttat ggacttggaa ctgctgatat gaaatctgga cttatagcat
     6301 caatattagc tgtaaagctt ataaaagata gtggtctaaa tgtacctggt aatgtaaaaa
     6361 taatgtcagt agtagatgaa gaaggtggcg gaaatggtac cataaatgca gttatgaatg
     6421 gtattggtgg agattgttgc ataatttgtg aaccatcaga acaaaactta atagttgcac
     6481 atatgggatt tgtattcttt gaagttgagg ttaaaggtgt gtctcttcac tgtggtagta
     6541 agtgggaggg tgtaaatgca attgagaagg ctatgttgtt gcttcaagat ataaaagagt
     6601 tggaacataa ctggcttatg atatataaac atccactatt accttcacct acaataaatt
     6661 taggtgtaat aaatggtgga acagcaggtt ctactgtgcc agataagtgt gtatttaatt
     6721 tatgtgtaca cttcttgcca aatataatga gttatgaaca agttgtaaat gatgtaacaa
     6781 atgtcataat gacaagggct aatggagatt tatggcttaa agataataaa ccaaatataa
     6841 acatatatca atcagggtta ggttttgaga tggacaagga ttctgatttt gtagtgaatg
     6901 cacataaaat actagaagaa actctaggaa aaaaattaga gataaaaggt tcaacagctg
     6961 gcaatgatgc gagagtaatg aaaaatttag ctgaaatacc aacacttata ttagggccag
     7021 gttcaataga acaatgtcat tcaattgatg aatatgttga aattaaggag tatttagata
     7081 gtatacttat gtatgcaagt ctaatattaa acttataaaa gtaggaggaa tatataaatg
     7141 aaaaaagtat taatagtagg ggaatcttgg gtaaaaaata taactcatat aaaaggattt
     7201 gatacgtttg ttacaactca ttatgaagaa gctgtaaagt ggttgaaaga agcaatagaa
     7261 tctggaggat atgagacagt tcatatgcct gcacatgtag cagcagatag ttttccgtat
     7321 aaattagaag agttaaatga gtatgactgt ataatattat ctgatatagg ttctaatacg
     7381 ttcttattat ctaatagtac ttttatagat tgcaatagta atccagatag acttgaacta
     7441 atcaaagagt atgtaaataa tggtggggcg ttaatcatgg taggtggata tatgtcattt
     7501 acaggaatag atgcaaaagc tagatttgga gaaactgcta tcaaagatgt attaccaata
     7561 acaatgatag ataaagatga tagagttgaa aaaccagcag gaataattcc agaagttatt
     7621 gaatctgaac atccagtatt aaaaggtata ccaacagaat ggccaaagtt tttagggtat
     7681 aacaaaacag tagctagaga taattgtcct gttttagcaa ctattggagg agacccattt
     7741 gtagctgttg gcgagtttgg aaaaggaaaa tctgctatct ttagttctga ctgtgcacct
     7801 cactggggtc caaaagaatt tactgattgg aaatactata ataaattatg ggttaatatg
     7861 cttgattggt taacatgcta aaaattttat aaaacttgaa aacctcttac ttgataaaat
     7921 gaaacttaca agtcaagagg ttttattgta taaaatggtt gtatggtaga aaatattttt
     7981 aaatcagtta aaattagtca ttttggctga taatctataa gttattagaa acagggaggt
     8041 ttatataatt aatatggata atttaaataa aaagtttttg atggaatata tagatgagat
     8101 acagggagaa ttaagagata tatcagataa gatatggaaa aatccagaac ttcagtataa
     8161 agaatactat gcttcaaatt tacaaaaaga atgtttaaaa aaacatggtt ttagtataga
     8221 gggattagaa ggtatggata cagcatttgt ggctagttat ggagaaggta gaccagttat
     8281 agcagttttg ggagaatatg atgctttaga tggtttgtct caaaaagtat ccactattaa
     8341 agaacctatt gtagatggtg gtgcaggtca tggatgtgga cataacttac ttggtacagg
     8401 ttcaatagga gctgtaattg cagtaaagga attaatcaaa ctaaaaaagc tgaaagggac
     8461 tataaaatac tatggatgtc cagcagaaga agatttatct ggaaaagtat tgatgataaa
     8521 aaaaggtttt tttgatggaa ttgattgtgc ttttagttgg catccatttg atataaatac
     8581 tcctataaga atcccaacac ttgctaatta ctctgtgaaa tttagatata atgggattag
     8641 tgctcatgca gcacaagctc cttataacgg aagaagtgca cttgatgcag tagaacttat
     8701 gaatataggt tgtaattatt tgagagaaca tatatttgat agttgtagag tacactatgt
     8761 aactactaat ggtggaaaaa tgccaaatat agttccagat tttgcagaag tgtggtatta
     8821 cataagagga gttaaaatgg agcatgtaag agatgtattt ggtaggattg ttgatatagc
     8881 aaaaggagca gcattaatga ctggtactat tatggaatat gacattataa gtggagtata
     8941 tgactatatt ccaaatacaa tattaacaga tattctatca caaaatatga aattagtagg
     9001 tgtgcaaaac tataatgaag atgactacta ttttgcagat aaattagctg agacagtgtc
     9061 tatagataaa agaagtagtg tttctagtgt cttaagtgga aataaggata ttactaagat
     9121 gaatttacat gatgaggtta cagatgatac atttactcat aataactgta tatcattatc
     9181 attagatatt ggagatgtta gttacataat tcctacagca cagtgttctt gttcagtttg
     9241 gcctattggt atttctgccc atacatggca atcttgtgcc agtgcaggtt ctgatatggg
     9301 atttaaggca atgttattgg catctaaatc tatatcatgt tcaataaatg atgtaatgct
     9361 tgatgaattg gttataaata aagcaaaaaa agaattgaag gatactgtag gtagttttaa
     9421 atatatacca attatttaaa acttaaattg atataagcac aactaacttg tatgagttaa
     9481 tataagtata taatttaata ttatttttaa cccaaccttc taattatttt aaatttaaga
     9541 tacaatgatt agaaggaatt ttttaagttt cttagaatac tgtaaattat ataatcttga
     9601 taaaaagtgt tatactatat ataatctaaa taaaaaatag tacaaaaagg agtatttatg
     9661 tttggatatg ttaaaataaa caaaatggat ttaacattta gagaatatga ttattacaaa
     9721 gcttactatt gtggactatg taaatactta aaaagaaatc atggagaaat ctctaggttt
     9781 tctttgaatt atgatataac atttttaatt gttttattaa cagcagtata taacccagag
     9841 tctataagta cagaggaagt gtgtatagta aatccattta aaaagaaaaa ggtaattaca
     9901 aatgatatta cagaatatgc agcaagtatg aatattcttt taacgtatta taaattggaa
     9961 gataatttaa tggatgataa acgcataaaa gataaattgg catattatat atataaaaac
    10021 aaattaaaat tagcatatga aaagtatcct gaaaaagcag agtatataaa acaacaacta
    10081 aatgaattaa ataagttaga aaaagataaa aatataaata tagatgaagt atctagtata
    10141 tttggaaata taatgggtga agtatttgtc tataaaaaag atgaaaatga gagaaactta
    10201 agaatgatag gttttaatat aggtaagtat atatatctac tagatgccta tgaagattta
    10261 gatgaagatt ttaagaaagg aagatataat ccatttatag agtatattga taaaaatgat
    10321 gagttaaaag aaaaagtaaa aaaaataata gtaacatcat taggattttt agctagggga
    10381 attgataatc taaatctaaa taaaaatgtt ggaataatag aaaatataat ttattctggt
    10441 gtttatttaa gatatattaa tatattagaa agtagaggtg gaaagaatgt acagtaatac
    10501 ttataataat gaaaatttta tgagagcaag atcattcata gaaaatagtg aatttaaaaa
    10561 agcatatgat tttttaaaaa ctttgactga caaatgtgca gagtggtatt atttaactgg
    10621 tttttctgca atgaacatag gatattatga agaaggtgaa gacttcttaa aaagagcaaa
    10681 atttatggag cctgaaaata gtgaatatag tgatgcactt agaagttata cacaatatag
    10741 aaatgattat agcaataggg ctgacaatta taatagaaga agacgtaatg atttagatgg
    10801 atgttgttgc tgttgttgtg atgattgctg ttgttgtcta ggtgacgatt gttgtgaaaa
    10861 ttgtgctaaa ctttggtgtt tagattcatg ttgtgaatgt tttggtggag atttaataac
    10921 ttgttgttag gatgataaat atgagtataa aagttgctta tggaggagtt ttattagcat
    10981 taaatgtgat attgttgact cttacaaaca ttattcctgt aaatacattg tttattatgg
    11041 ggttagcatc acttttagta tcaattgtaa taatggaatg gggctttaag agtggaatcg
    11101 ctttttatat aggttctata gttttaggat ttatagtgat ggcaagtaag tctcaatgga
    11161 ttgtatattc acttaccttt ggaatatatg gtattgtaaa atatcttata gaaaaagata
    11221 ggtctatata tattgagtat tttatgaaat tagtatttgc aaatataatg atacttatat
    11281 tgtatttttt attaagaaca atagtttata ttccaataaa catatttatt ataggt
//
LOCUS       DACXMZ010000123        11331 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.124, whole genome shotgun sequence.
ACCESSION   DACXMZ010000123 DACXMZ010000000
VERSION     DACXMZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.124"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            105..710
                     /locus_tag="JD016_12385"
     CDS             105..710
                     /locus_tag="JD016_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAU5000432.1"
                     /translation="MDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVFC
                     PELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGAT
                     IAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQN
                     IQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            1037..3934
                     /locus_tag="JD016_12390"
     CDS             1037..3934
                     /locus_tag="JD016_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAU5000433.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            4255..4467
                     /locus_tag="JD016_12395"
     CDS             4255..4467
                     /locus_tag="JD016_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAU5000434.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            4480..4701
                     /locus_tag="JD016_12400"
     CDS             4480..4701
                     /locus_tag="JD016_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAU5000435.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            4801..6957
                     /gene="feoB"
                     /locus_tag="JD016_12405"
     CDS             4801..6957
                     /gene="feoB"
                     /locus_tag="JD016_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAU5000436.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            6977..7135
                     /locus_tag="JD016_12410"
     CDS             6977..7135
                     /locus_tag="JD016_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAU5000437.1"
                     /translation="MNMATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSS
                     KSCHSAKR"
     gene            7315..8025
                     /locus_tag="JD016_12415"
     CDS             7315..8025
                     /locus_tag="JD016_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HAU5000438.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            8110..8880
                     /locus_tag="JD016_12420"
     CDS             8110..8880
                     /locus_tag="JD016_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000439.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            8873..9604
                     /locus_tag="JD016_12425"
     CDS             8873..9604
                     /locus_tag="JD016_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000440.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            9605..10597
                     /locus_tag="JD016_12430"
     CDS             9605..10597
                     /locus_tag="JD016_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAU5000441.1"
                     /translation="MIKRNKKILAVMTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPK
                     EINLTYVKSPLNVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFL
                     HALGGTSAIIAASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTI
                     LHQLLIAALAKEGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKM
                     IVNGEGLVSGIIVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVG
                     LSLDETKEMYSWYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(10695..11291)
                     /locus_tag="JD016_12435"
     CDS             complement(10695..11291)
                     /locus_tag="JD016_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HAU5000442.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         4350 a         1168 c         2038 g         3775 t
ORIGIN      
        1 taaatgatta atccaaaaat aaatttgatt ttagaaaagt aaattgacat gtaaagaaga
       61 atatattaaa ctaagtataa aaaatttgct gttttgtaaa ggtggtggat aatctgataa
      121 agtatataca taaaagtgat gtagctattg ttgttttgca tgaaatctat gggattaata
      181 gctttataga agatgtttgt caaaagtatt ataaatatgg atatgatgta ttttgtcctg
      241 aacttctggg agaaaaaaga gtttttccat attcaaaagt ccaagaggca tatgattttt
      301 ttataaataa tgttggtttt gatatttata aaaaagttga aaagcttata aatgatttaa
      361 aatcagagta taaatatgta tttgttgctg ggtatagcgt gggagcaact attgcatgga
      421 gatgcagtga agattctttt tgtgatggca taatttgttg ttatggctct cggattagag
      481 attacatgga tgtaattcca aaatgtccta ccttattagt atttgcaaag cacgattcat
      541 ttgatgttga ttctatttct taccaacttt ctaggaaaca aaatatccaa attgagattt
      601 tagatgcaaa tcatggtttt atagatagat attcaaataa ttattctgat attcaaacaa
      661 aagtatttac tattaaagaa aaaagttttc tgtctaaata tttagcatga tttagattat
      721 tataaaatat atagaaagat ttaattaatc tgaaaagttt ttatgacact attgatattt
      781 aaaatattag tagtgttatt atattttttg aaataaaatt aatcactaga attatagtgt
      841 tagttgtgct tttatgactt ataagaaact aatattagat agtgtattta aatagataat
      901 aataaattca ttatagttga ataagaatat tatatgagaa tattgcaatt tgatatattt
      961 accattacaa taaaagtagt tacaactaga aaacgaaata agttaaagta agttggcaaa
     1021 ttggaggttt ttaaatatga aagcatttaa aaagcacttc ttgatacttt ctttgatatt
     1081 tttacttatt atatcatgtt ttaacaatat actatgcttt gctgatgagt ctgaaaattc
     1141 aaaaaaaata agagttggat attgtgatga ctacggaata atatcctcat caaaagagca
     1201 ttcttataca ggatatggtt atgattatct aagagaaatc tcaaaatata caagatggga
     1261 atatgaattt gtaaaagggt cttgggaaga atgtttagac agactagaaa agggtgaaat
     1321 agacttactc ggacctttac agaagaatga tgaaagaaat aaactattta attttcctaa
     1381 actgagttca ggatatgaat atagtgcact atatacaaag gatagtaaca ataatatgtt
     1441 ttatgaagat ataagttcct ttaagggaat gagagtagct gtacttagag gaaactttca
     1501 taatactgca tttgagaagt atagagaaga aaataatttt tcagttgaat atatatactg
     1561 taattctatt gatgaattaa ttgagtccgt caatgaaaaa aaggcagatg catttgtttg
     1621 tggaagtatt ataaatgcta aaggaatgaa aatagttagc aaattttcag tagaaccttt
     1681 ttattttgct actgcaaaag ataaacctaa tttggtaaaa gaattagatt atgctttaaa
     1741 agaattaaaa ataaatgata tgtattatga gttggaatta tataaaaaat atttcaaaag
     1801 agaggtcaat aattccatag catttactcg gcaagaaatg aattttatta aagcaaaccc
     1861 aaaacttact atggtatatg attcagagtg gtctcctgta gagtattatg ataaagaaag
     1921 caattcattt aaaggtataa gttccgattt aatgtcaatt atatcaaaaa aatgtggtat
     1981 aaaatttgaa tatattaaaa caaaaaatta taatgagagt cttgattaca taaaaagtgg
     2041 taaggcaact atgctatgtg gctcaatcaa tgaaaatgat aaggcaaaac gatttaatat
     2101 gaaattgaca aatccataca taaatattcc aatgattatg gtaggaaaat tggataccaa
     2161 tctcaataat gatttaaata ttgctttaac tagtagttat aagtctattg attcttatat
     2221 agaaaagagt tttgaaaatg ctaagacaac atcttataag agtgtagaaa gttgtctaaa
     2281 cgctataaat gaaggtaaag caaacttaat gatacttagt tcttatcagt ttgatgaact
     2341 tttaagagaa ggaaagagtg agaatttatc tgttatatct gttttagata caagctatgg
     2401 gatgcgtata ggggtatcaa ataaaactga ccctatatta gtgtctattt taaataaatc
     2461 aatagacaaa attactgaag aagaattaag tgattgtata tattctaata ctattgacaa
     2521 accttataaa gtaccatttg gtgtgatttt taaagaatat tcaatacaaa ttatttcttt
     2581 tgtatgtatt ttattcttaa ttgctattaa atatataatt tataataaaa agaagaaaga
     2641 ggattatcta aggaagatag catatacaga cccattgaca ggagcagata gtatagataa
     2701 atttaaaata aattctaata aactgtttgc taagaataat ccagaagaat atgcgttgtt
     2761 ttatatagat gttgacaaat ttaaatatat aaatgatatg tttggatatg atatgggaaa
     2821 tgatactttg atacatatat caaatactat tgcaagtgaa ttaaaggaag atgaaatttt
     2881 tgcaagagtt tctgctgacc attttgttct tcttattaag tataagacag atgatgatat
     2941 taaaacaagg ttaaacaata tttacaataa ggttcaaata cttagtaatc ctaaaataaa
     3001 ctattataaa ttgattttag attgtggtat atataaaata agtaaaagtg ataatgacat
     3061 aaatactatt atggatagag ctaatactgc aagaaaaact ataaaaggtg gtcataaaaa
     3121 ttcatttgct ttttacgata aggaaatgca caaaaaaata ttaaaagaga aagaaattga
     3181 aaattcaatg gtagatgctc ttaataatgg agaatttatt gtttattttc aacctaaata
     3241 tagcctttca gattatcaaa ttataggggc agaggctctt gttcgttggg ataatccaca
     3301 aaaaggtcta ataccaccaa tagaatttat tcctgtattt gagaggaatg gttttattgt
     3361 aaatattgat ttctatgtat ttgaggaagt gtgtaaaaag attagagaat ggatggatga
     3421 aggacaaaaa gtagtaccta tatctgtaaa cttgtctcga atgcattttg taaatagtaa
     3481 ttttatagag gagtttaaat taattgtaga taaatacaaa attccgacaa gacttataga
     3541 attagagcta acagaaacag cagtattaga taatatagaa ggattacttg atacaatgaa
     3601 caatttgaaa gaaaagggat ttgtaatctc tatggacgat tttggtacag gatattcctc
     3661 tcttaatctt ttaaaagaat tgcctgttga catattaaaa ctagatagag cattttttac
     3721 agaaaaagat gagagtaata atgaaaaaat tgttatatct aatgtcatta aaatggcaaa
     3781 ggaactgaaa atgaaagtaa tatcagaagg tgtagagact atttcgcaag tagaatttct
     3841 aaagcaaatt gggtgtgata tggtacaagg atatttgttt tctaagccaa tgcctgtaaa
     3901 agagtttgaa aaaatcgctt ttaaaaaaga ataaagcaat gtgtaaataa tgacatatat
     3961 aataaaggtg ctttaatatt agttgagtat actattttag agcatcttta tttttgtatt
     4021 aatttttacg attttattaa taaaataaat caaatgagaa aaaagatcaa aagaatacaa
     4081 taatcacttg caaaaaatat actattgtga tagacttaaa tcaaaattag gaattgtaaa
     4141 aagaaggaaa aaataggata ttattttaat agattaagat ttaaggtatt gtaatttata
     4201 tataagataa cgattgcttg tagatttaaa ttactttaag aggagggata ttatatgatg
     4261 ccattgatat tatcaaatac aggtgaagaa gtagttataa agaaaatttc gggaagtgat
     4321 aagacgagga cttttttaaa tagtataggt tttgtagtgg gtacaaatgt taaaatagtt
     4381 tctaagatag atggtaactt aataatagac gtaaaagata ctagagttgc attagataaa
     4441 aaaatggcaa gtagaataat aatataggga ggtaatgaca tgaacagact gaaggatatt
     4501 aagtgtggag agactgtaaa agtcaaaaag ttagaaggag aaggagctac acgaagacgt
     4561 ataatggata tggggattac aagaggtgtt aatatattta taagaaaagt agctcctctt
     4621 ggtgacccaa ttgaggtaac agtcagagat tatgaactat caattcgtaa gtcagatgca
     4681 gaaaaaatta ttgtggaata ataaagatta attagagtag ggttaatgta attatataat
     4741 gagaatgatt ttctgtatca caaacagtga tgtatttaaa taataatgga gggaatggaa
     4801 atgtcaataa aaataggtct cataggaaat cctaactgtg gtaagacaac aatgttcaat
     4861 gggcttacag gctcatcaca atatgttggt aactggccag gagtaacagt tgaaaaaaaa
     4921 ggtggaaaat taaaaggaaa taaagatgtg gaaatagtag atttaccagg tatttattct
     4981 ttatctccat atacattaga agaagtagtt acacgtaatt ttatgctaga tgataagccg
     5041 gatgcagtaa taaatattgt agatgcatca aatatagaga gaaatttata tcttacaact
     5101 caagttttag agctaggtat accgaccgtt atagctctta atatgatgga tattgtaaat
     5161 aagaatggtg ataagataaa tattaaagaa ctatctgaag ttataggttg cccagtggta
     5221 gaagtaacag cagttaaagg tcaaggaatt atggaggctg ctgaaaaggc agttgaactt
     5281 gcaagtagta acaataaatt aaatttcaaa ttaccctttg ttgatgaaag taaggatgca
     5341 atagaaaaaa ttgaaaaaat aatagaagaa aagactccat atatagatgt agaaacacgc
     5401 tggttagcta taaagctatt tgagagagat gaaaatgtta tacaaaaact tgatatttct
     5461 aaaacaatat tgaatagtat tgaagaaata actagaaatt gtgaagatga attggatgat
     5521 gatagtgaaa gtataataac agccaatcgt tatgaattta ttagttcaat aatttcaagt
     5581 ataataaaga agaatcgaaa aggtaaggaa actgtatcag ataagataga taagattgta
     5641 acaaatcgta tactagcatt acctattttt gcacttataa tgtggggagt ttactatata
     5701 gcagtaagtt cattgggtac tatagcaact gattggacaa atgatgtact gtttggagaa
     5761 attattcaag gcaatgtaag taattttcta gcttctttaa atgtagcaga gtggttacaa
     5821 ggacttgtag ttgatggtct aattggtgga gttggagcag tactaggatt tgtaccacaa
     5881 atcatgcttt tattcttatt attatcaata ttagaagatt gtgggtacat gtcacgtgta
     5941 gcttttataa tggatagaat tttccgtaag tttggtcttt caggaaaatc atttatacca
     6001 atgcttataa gttcaggttg tggagttcca ggagttatgt caactcgtac tatagagaat
     6061 gatagagata gaaaaatgac tattatgtta actacattca ttccatgtgg agctaagatt
     6121 ccaataatag cattatttgc aggggcatta tttggaggtg cttcatgggt agctccatca
     6181 atgtatttct taggtattgc aatgataatc atttgtggaa ttatattaaa gaaaactagt
     6241 ttatttgcag gagaaccatc tccatttgtt atggaattac ctcaatatca tattccaagt
     6301 gcaaaaggtg ttttaataca tatgtgggat agaggaaaag catttataat caaagcagga
     6361 acaattatat ttgttgcttg tggagtaatc tggttcttac aatcatttaa ttggtcatta
     6421 caaatggtag atgctggaga tagtatatta gctagcttag gaaatatagt tgcaccaata
     6481 tttgctcctc ttggatttgg aaattggcaa tcatcagtag caacagttac aggtctagta
     6541 gctaaggaaa atgtagttgg aacatttgga gttttatttg gtatatcaga tgcaacagag
     6601 caagacccaa cacttcttgc ttcagttgca agcatgttta cagtagctag tgcgtttgca
     6661 tttatggcat ttaatatgct ttgtgcacct tgttttgcag ctattggagc cataaaaaga
     6721 gaaatgggtt catggaaatg gacatggatt acattaggat tccaaacttt aacagcgtat
     6781 ataattgctc ttcttataaa tcaagttgga agtttagttt taggtactgg aggaagtatt
     6841 gctggggcaa taatatctat atttatagca gtagcagtag tctttgtagt tttaacttat
     6901 tcaaataaaa atatgaaaaa agaaaaaatg ggtaaattaa gttatatgaa aaattaattc
     6961 atataaataa ggagtgatga atatggctac atttattata gcagctattg tagtagtatt
     7021 aatggcacta gctgtaatgt atatggttaa aaattcaaaa aagaatggta gcagttgtgg
     7081 atgtggttgt tctggatgct caagtagtaa gagttgtcat agcgctaaga gataaatagg
     7141 ataagagcct gataaaaagt taaattgctt tattatcagg ttcttatttt ttgtataaat
     7201 aacaaatttc atatttgaag taggttagac tatgctgtaa ttttactgta taattatata
     7261 taaatatatg attaggaggg attattgcta aattaagcaa tataagtgaa tattatgttt
     7321 aaatttgatg ttacaaacga gcttatgtgt atagcaaggg gaagtggtaa attttttgca
     7381 aaaaaaggtg ctatggtagc ctttaaagga aattttaatt ttgaaaaatt gctgttaggt
     7441 ccaagtaatg gcggtggatt gggaagagca ttattaggtc atgttcagag gtcattgaca
     7501 ggagaacaaa tgccaataat ggtggttgaa ggtgaaggtg aaatttacct agctcaaaat
     7561 gcttaccatg tagatgtaat atcaattgac ccaggtgatt ccatttatgt tgagagtgaa
     7621 aacttattgg cattttctga acaattaaat tacagtgtaa aattgattgg ttcaggggta
     7681 atatctcaaa aaggtctttt tactacacat ttagtaaata aaacaggtca aaatcaagat
     7741 gttgctataa taacagatgg taatcctcta attcttgaag gaccttgttg tgttgaccca
     7801 gatgcattag tagcttggac tggaagagaa ccatatccta aagtatcaaa actatcatgg
     7861 aagactttta ttggtcagac ttcaggagaa tcttatcata tggaatttgt tgaacctgga
     7921 caaatagtca ttatacaacc atctgaaaga ttgtctggct taaaaacaga ttctacacct
     7981 tctgatagaa tgagttcagg tggattaaga cttggcatag attaaatctt atgatatagc
     8041 ttaattatta tttaagatat taaaaaataa aaagggtagc agggatagat atgttaaaag
     8101 gagttttata tgtttgagaa aattcataaa aggcttaaag gatattatat ttttttaata
     8161 attttaattt tatggaagat agtatgtgtt ttgggtatat ggagtagcta tattctgcct
     8221 cctcctgaaa tagtcttaga tacttttata aaaatgatat atgacggttc tattttctta
     8281 aatgtttatg ccagtgtaag aaggatatta ataggttttt taataagttc attgatggct
     8341 ataccacttg gagttgtatt tggagtaaat aaaaaaatgt atgaatattt caaaccacta
     8401 tttgaattta tgcgaagtac acctccacta gctttggtgc caatgcttat tttatggttt
     8461 ggtataggtg aagaatctaa aattataatt attattttgg catccttttt tccaatattt
     8521 ttaaatacat tgaaaggtat aaaaaattgt gacaataaat taatagaagt aggtaaatct
     8581 tttaatttat ctaaaaagag aattttttat aaaataataa tccctaattc tatgctggat
     8641 attgttgttg gacttagttt agggcttggg tatagtttta gggctataat tggagcagag
     8701 cttattgcag cttcttcagg actaggatat ttgatttctg atggaaaaga tatgtctaga
     8761 actgatgttg tattggtagg aatttttgtt attggatttt taggtatact ttcagattat
     8821 ctattttcaa agataataac aaaatttagc aaagggaagc aggttgattt aaatggttag
     8881 aagtggattt ttgctagaga atatatataa gaaatattta gttgataata aagagcattt
     8941 agttcttgat aatatatctt taaatatatc tagtgaagag ataactgtta tattaggtga
     9001 aagtggatgt gggaaaacaa cgctacttag aattttagca gggcttgaaa atgctactag
     9061 tggaaacata tattttttta ataatgataa aaagtgtact ccaaaagtag gaatggtatt
     9121 tcaagaaagc agacttatgc catggctgaa tgtaagtgaa aatatacttt tacatactga
     9181 aaaagataat agaaataaag ttgatttaga caagtattta aaaatgatga agcttgaaaa
     9241 gtttaaaaac tcatatccaa atgaattatc aggaggaatg gctcacagag taagtatagc
     9301 aagagcactt tccttcaatc ccgatatttt gctgatggac gaaccatttg cagcactaga
     9361 ttacttcaca agaagaaaaa tgcaaaaaga agttgtaaat atccataaaa atactaaaaa
     9421 aggtgtagta tttgtaactc acaatattga agaagctatg gaaatagcaa agaaaataat
     9481 tgtttttagt aaaaataaaa ggattaaaca atttagtgtc gaagatgaat ataatagaga
     9541 tttaactaaa aactattata taaacttgaa aaaagaaata ttaagggaat tgggggagtt
     9601 ttaattgatt aaaagaaaca aaaagatttt agcagtaatg acatctttaa taatggtgtt
     9661 ggttgggact gtaggatgtt caacaacaaa tgataagaaa gatgaacctg caaaaaaaga
     9721 ggctagttta cctaaggaaa taaatttaac ttatgtaaaa tcaccactta atgttcctag
     9781 tatattagaa aaaaaggatg atttatttgg aaaggaattt aaaaaggaca atataaaagt
     9841 caacttccat gaattgacaa caggaccaga gcaaactcaa gcattagcag caggtgagct
     9901 agacttttta catgcattag gaggaacgtc tgcaataata gctgcatcaa atggtgttgg
     9961 tcttaaaatt acgaatattt atagccgttc accaaaagga tatatgctta taactaataa
    10021 tgataatatt aaatctataa aagacttaaa aggtaaaaaa attgtaggtc caaaagggac
    10081 tatacttcat caactgttga ttgctgcact tgctaaagaa ggctatacta tagatgatgt
    10141 tgaatatgta aatatggata ttcctagtgc tgcttctgca ttagaaagta aaagtgctga
    10201 tgtagcaatg cttgcaggtc cagtggcatt gaaaacgata aattcaggtg ccaaaatgat
    10261 tgtaaatgga gaaggattag taagtggaat aatagttact gctgtaagtg atgactttgc
    10321 tgaaaaatat cctgaattag taaaaagatt tatgaaggtt catgaagaaa ctttaaaata
    10381 tatgaatgaa aacaaggatg aagtgatgga tgttgtatct aaagaagttg gtttatcttt
    10441 agatgaaaca aaagaaatgt attcttggta tgactttagt tctaaaataa cggacaagga
    10501 tataaaagaa ttagaagata cacaggaatt tttgatgtct aatggaatgc aacaaaagaa
    10561 aataaatatt aaagatatgt tgtacaatca aaattagaat actttaaaat attaaagtgg
    10621 aaaatacacc cttatttatt atggaaactg tatggtaaaa gagggtgtat ttttattatt
    10681 atacaaaaac acaattaaaa acagcttact attttatcta cacttatgta gtctagtaat
    10741 tttctcattt tcttacggtg aagtaaaaac ttcgaatatg tgtcctcgga ttgttttgtg
    10801 tcaccataaa ctttccaata tttaatcata gaaacttcat aattactata tcccataaat
    10861 ccaatcacat ctttaagagg atatcctaaa aaaataccta tttcatcagg aaaaacatca
    10921 ctcttaagtt tatctactaa atgctctaaa taagcattaa cattcgtatt ttgtttgtat
    10981 cctaaaaact ttaaaaagtt tactgttttt ttacatttta atttttctga taatgctttt
    11041 ttatttacaa ataagatttt taatcccttt ttttctttat caataactat aaaatctatt
    11101 ttcttacatt tatatagata tttataaata tcattaattt tttcctctct atttacatca
    11161 gtaaacgaaa tatttattat ttcagcaggt ttagagccta aaaagactgg acctaaaact
    11221 tctaaaagcc attttatata tgatgattca attttatttt tacaacaaaa ttgtgaatta
    11281 caacaagaca taaaagataa cctccttcaa atgaaaatta atatcaagta a
//
LOCUS       DACXMZ010000124        11249 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.125, whole genome shotgun sequence.
ACCESSION   DACXMZ010000124 DACXMZ010000000
VERSION     DACXMZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.125"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(118..1293)
                     /locus_tag="JD016_12440"
     CDS             complement(118..1293)
                     /locus_tag="JD016_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAU5000443.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(1380..2225)
                     /locus_tag="JD016_12445"
     CDS             complement(1380..2225)
                     /locus_tag="JD016_12445"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HAU5000444.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(2323..3120)
                     /locus_tag="JD016_12450"
     CDS             complement(2323..3120)
                     /locus_tag="JD016_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAU5000445.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(3139..4149)
                     /locus_tag="JD016_12455"
     CDS             complement(3139..4149)
                     /locus_tag="JD016_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAU5000446.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(4168..4950)
                     /locus_tag="JD016_12460"
     CDS             complement(4168..4950)
                     /locus_tag="JD016_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAU5000447.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(5009..6145)
                     /locus_tag="JD016_12465"
     CDS             complement(5009..6145)
                     /locus_tag="JD016_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAU5000448.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(6854..7321)
                     /locus_tag="JD016_12470"
     CDS             complement(6854..7321)
                     /locus_tag="JD016_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HAU5000449.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(7321..7398)
                     /locus_tag="JD016_12475"
     CDS             complement(7321..7398)
                     /locus_tag="JD016_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000450.1"
                     /translation="MKNKSIIDIIKNNMKFIKVQTRRGI"
     gene            complement(7400..7942)
                     /locus_tag="JD016_12480"
     CDS             complement(7400..7942)
                     /locus_tag="JD016_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000451.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(7994..8680)
                     /locus_tag="JD016_12485"
     CDS             complement(7994..8680)
                     /locus_tag="JD016_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAU5000452.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(8680..9540)
                     /locus_tag="JD016_12490"
     CDS             complement(8680..9540)
                     /locus_tag="JD016_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000453.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            9675..10475
                     /locus_tag="JD016_12495"
     CDS             9675..10475
                     /locus_tag="JD016_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HAU5000454.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            10500..11069
                     /locus_tag="JD016_12500"
     CDS             10500..11069
                     /locus_tag="JD016_12500"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAU5000455.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT         3606 a         2056 c         1276 g         4311 t
ORIGIN      
        1 ttgtttaata gataataatt atttttaact ataaaaacct gtaagaataa ctatttgtta
       61 ttcttacagg ttaaattcat aattaaatat ttaatttgta aatattataa tctaaaacta
      121 tctcttaact attaaagtag ttcccattcc accacctata caaagtgtag caagaccagt
      181 tttagcatct cttctcttca tttcatataa aagtgtagta agtattcttg ctcctgagca
      241 tcctattgga tgtcctatag ctattgctcc accattaaca ttaactttat tcatatctat
      301 atttaagtct cttattacag ctacagattg ggcagcaaat gcctcattag cttcaactaa
      361 atctatatct tcaatagtca tattagcagc ttctaaagct tttttagttg ctggaactgg
      421 tccatatccc attattttag ggtcaacacc agctgttcca taagaaacta tagttgcaag
      481 aggctctatt cctagttctt cagctttttc tttagccatt actactagca tagcagcacc
      541 atcatttatt cctgatgcat taccagcagt aactgttcca tcttttttaa atgcaggtct
      601 taacttagca agtttctcca ttgtagtgcc aggcttaata tattcatctt tatctactac
      661 agtgtcacct tttcttcctt ttataacaac aggaactatt tcttcatcaa attttccttc
      721 agcttgagct ttttcagctt tattttgact tgcaagagct aattcatctt gttcttctct
      781 agttatattc cattgctctg ctatgttttc agcagtaata cccatgtgat agttattaaa
      841 tatgtctgat aatccatctt ttatcattga atcaacaaaa gcagcatcac ccattcttgc
      901 accatatctc gcacttggta ctaaataagg agacatactc atgttttcag ctccaccaac
      961 taacattata tcagcatcac ctaatgctat aagttgagat gccattgaaa cagatcttaa
     1021 tccagaacca caaactatat ttatagtcat agctggtttt tctactggta ttcctgctcc
     1081 taatgctatt tgtcttgcta tattttgtcc aagacctgct gtaagtactc cccctaaaag
     1141 agattcatct atcatatctg gagttatgtt agctcttttt atagcttctt tagctgctgt
     1201 tacccctaac tctaccgctg aaactgattt aaatgctcct ccaaaacttc ctactgctgt
     1261 tctagctgca ctggcaatta ctacttctct catattcatt acccccatta aattagattt
     1321 ttcatatatc cgaaattgtt ttttaaacaa ttttatttaa acttttaatt cccaaattct
     1381 tatttattat aatcatagaa tcctatctta gtttttcttc ctaattgatt agctctaacc
     1441 attttagcta aaagtggatg aggtctatat ttagtatctc caaactcagt atataaaacg
     1501 ttcattatag ctaaaacaac atctaatcca attaaatcac ctaatgctag tggtcccatt
     1561 ggatggtttg ctcctaattt catagcttca tctatttctt ctttacttgc aacaccatct
     1621 gcatatatac caacagcttc atttatcata ggtataagta ttctatttac tacaaatcca
     1681 ggagattcag atacatctac tggtacttta ttgatactct tagataattc aaatactgta
     1741 tcaaaagtaa cttttgatgt taactgacca cttataactt caactaattt catcatagga
     1801 actggattaa agaaatgcat tcctataact ttatctggtc tcttagtaga agaagctatt
     1861 tctgttatag ataatgatga agtatttgtt gccaagatag tatcttcttt acataattca
     1921 tctagtaact tgaaaacatc tttctttata ttcatgtctt ctacagatgc ttctattatt
     1981 aaatccatat cttttaaatc ttcatagtta gtagttgaac taacatgact taatatttct
     2041 gcttttgtag cttcatccat ttttccctta gtaactaact tagttaaatt tttatctaat
     2101 aaagctaaac atttatctat agcaccttga gttctactct ttaaacatac atcatgtcca
     2161 caacttgcaa aagtttgtac aataccactt cccatagttc cactacctat tacagctaat
     2221 ttcatagtta taacctcctg ttattttagt atttatattc tgttaactag attaaaatac
     2281 tatttaatct agttaacagc aaatatattt ttaattagat ttttaccctt ttataaagtt
     2341 agcttctctc ttttcaacga aagctgacat tccttctttt tggtctttag ttgaaaaaca
     2401 aagaccaaat aaattagatt ctatatctat tccagtattt atatcagttt gagcaccaag
     2461 ttgtattgct tctttagagt atctaactgc aagctgagca tttttagcta ttatcttagc
     2521 taatttctca acttcttcta ttaaaatgtc tggctcaacg actctattta ctagccctat
     2581 tttttcagct tcatcagctt ttataacttg acctgtaaag attaattctt ttgcttttgc
     2641 cattccaacc aatcttgtaa gcctttgagt tcctccatat cctggagtta ttccaagagt
     2701 tacttctggc tgaccaaatt tagctttagc agatgcaatt cttatatcac atgccattgc
     2761 aagttcacat cctccaccta aagcaaatcc gtttacagca gctatcacta cttttttact
     2821 attttctatt tctccaaaag cttttgctcc taagatacta aaatctttag cagctacagc
     2881 atctaaatct ttcatgtatg caatatctgc tccagctaca aatgcctttc cttcccctgt
     2941 caatattaca acatcaatag tttcatcatt attaatatct acaaatactt cataaagttc
     3001 ttctaaagtc tttgaattta ttgcattaag ggctttaggt ctattcattt tcactgtaca
     3061 tatatttcca tctacttcaa cagctacatt ctcataaact ttaacatcac ttgtactcat
     3121 aataccctcc ccaagaaatt agttagctaa aacttcacct ttttcttttg caacacttaa
     3181 ctgactgata agttctggaa gcactttatg aacatctcca actataccaa catcagcata
     3241 tttaaatatt ggagcttctg gatttttatt tatagcaact ataaactcag catcttccat
     3301 accagctata tgttgtattg ctccagatat accacatgct atataaaggt ctggtcttac
     3361 agttttacca gtttgaccaa cttgtcttgc tttatctaac caacctgcat ctatagtggc
     3421 acgagaacca gaaacttctc cacctataat ttcagctaat tcataaagta tgtctaagtt
     3481 ttcttttcca cccattccac gtccagcaga aactaatatc ttagcatctt ctattttaac
     3541 ttgtttttta gcttctttta ttacttgtac aacttgaact aatttatcag catcattaaa
     3601 ttctaccttg aaacggttaa ttacagcttc tttagtttca tcaggttcat ttttcttcat
     3661 aacccctggt ctaactgtag acatttgagg tctgaaatct ttacaaacta ttgttgccat
     3721 tatatttcca ccaaaggcag gtcttgtcat taataataat tttgtatctt cagctactgc
     3781 aagacctgta cagtcagcag taagacctgt atgtattcta gcagaaactc taggagctaa
     3841 atctctacct attgaagttg caccaaataa tacaactata gggtcagctg cttttattgc
     3901 ttcataagct gcttttgtat atggttcagt tgtatacact gctaaagctt catcatctac
     3961 tactattacc tcatctgcac catagtgtgc taatgtatct attaaacctt ctaccttact
     4021 acctaaaagt aatgcagaaa cttttgtatc ataatctttt gctatttctg tagcctttcc
     4081 tagtaattct aaagaaacag tttgaattac attttctctt tgttctatta ctactaaaac
     4141 gttacccatt ctttatcccc ccttaaatta tatgatatac ttctctttta atttatctat
     4201 gataattcca gctgatgttt tcgcatcttc attgtatatt gtaccagctg gtttaactga
     4261 ttttgtaaat gatttaaata cactagttgg agaaccttta agacctaaat tagaagggtc
     4321 aacttctata tcttttacag tccatgtttc tactacatca ttttcgaaag catcatatat
     4381 tcttccaact ttcatgtatc ttggtgtgtt catatcttta agagttgtta taaggcatgg
     4441 cattttaact tttaagtcat ggcaacaatc ttcaaattgt ctttttacta atacatattc
     4501 accttcagtt tttatttctt cagcatatgt tattgatgga agatttaaat gttcagctat
     4561 ttgaggtcca acttgtgcag tatctccatc tatcgcctgt cttccagcta ttataatatc
     4621 aaaatctata ttttttaatg ctcctgctaa tgctgatgaa gttgcccaag tatcagcacc
     4681 tgcaaatgct ctatctgtta ataatatacc tctatctgca cccattgcta aagcttcttt
     4741 taaagccata tctgcttgag gaggtcccat tgttataaca gttacatgag cacccatttc
     4801 ttcttttaat tttatagctt cttctaaacc tgctttatca tcagggttta ttatacttgg
     4861 tactccatct ctaattaaag tacctgtatt aggatctagt ttaacttctg ttgtatctgg
     4921 aacttgtttt atacaaacga ctatattcat aaatccttcc tccatatcta ttttaatatc
     4981 gacctattgt gtatatttta atatttatct attttaataa ttttcctgaa ataaccattc
     5041 tttgaacttc actagttcct tcatatattt cagttatctt agcatctctc atcattcttt
     5101 ctactggata gtcacgagtg tatccatatc ctccatgaag ttgtacagct tttgtagtaa
     5161 cttccatagc tgtttcagct gcaaataatt ttgccattgc tgcttctact ccataaggtt
     5221 ttcctaagtc tttatttata gctgcttgat atacaaggtg tctagccgct tgtaccttaa
     5281 cttccatatc agctaattgg aattgtgtat tttggaattt tgataatggt ctaccaaatt
     5341 gtactctttc ttttacatat ttaacagttt catcaagagc accttgtgct aaacctaaag
     5401 cttgtgcagc tataccaatt ctaccaccat caagagtaga cattgctatc ttaaatcctt
     5461 gaccttcttt tccaagtaaa ttttctttag gtattctgca atcctcaaat attaattcac
     5521 tcgtagctga acctcttata cccattttct tttctttaac tccaaagcta aacccaggag
     5581 ttcctttttc aactataaat gctgatattc ctttgttccc cttagattta tcagtcattg
     5641 ccattactac atatatgtca ccagctattg cgtttgttat aaatattttt gagccattaa
     5701 gtatgtattc atccccgtct aaaacagcag ttgtttgttg gccagacgca tctgtaccag
     5761 cattaggctc agtaagacca aatgctccta atttttctcc acttgctagt ggtcttaaga
     5821 atttttgttt ttgttcttca ttaccatatt gatatatagg ccatgagcca agagatgtat
     5881 gagctgataa tataactcct gtagtaccac aaactctaga caattcttca actgccatta
     5941 tatatcctac agtgtctcca ccttctccac catattcttt tggatatggt atacccatca
     6001 ttcctgcttt tgccattttt tccactgttt cataaggaaa tctttcttct tcatcaagtt
     6061 ctgttgctaa aggtttaact tcattttcag cgaagcttac atatagctct ttaagcatct
     6121 gatatttttt agaatttaaa tccataatag cctcctcagt attttataaa tattttattt
     6181 tcatttatta attaaaatct cggcaacatc tataataagg tatttgaata tacgtcgaga
     6241 aatttataac cttaatcaaa ctacgctcta tatttatatg tgattatact aaccaattat
     6301 gacattaatt taatcataaa ttgttaattt aaaatcaact atacttaaaa aaaatctcgc
     6361 tttttggaat tttgtgaaat ttcaagttag aataagattt ctttcacact ggttacagat
     6421 aaaataattt gtttacgata tttatattag ggtatattaa acattaaata ttgtctgtac
     6481 atctgttttt tagccatgta cagaagattt ttatcgtata aaaattattt taatagagct
     6541 aaataatcgt atttttatct gactattttt tatttttaaa tatcttacat ctataatagt
     6601 atcactcaaa ggataatttt tcaataattt ctaattattt ttttaacaat atttattaca
     6661 ttttttccac ttttttgtca tttttttatt gtttcgaaaa aaaatcagaa ttttttaact
     6721 ttattttgga ttattagttt aaattagatt ttcctcttag atttataaat atttacacaa
     6781 aaaaactacc tctgtataga ttgtatacag aggtagtttt ttactaattc ctactatagt
     6841 ttactatatt tttttaccaa ttcttacaaa catctaccca tttttcacac ttagaaaact
     6901 tctccatttc ttcacatgta agttctattg cagagttact actgccacat gcaggaaata
     6961 ctgtatcaaa tcttttcatt gaatcatcta aatagacttt tatagaattt tttaaaccaa
     7021 atggacagac tccaccaata gcatgtccag taaactccaa gacttcttct ggtgtcaaca
     7081 ttttagcctt acaattaaat tcagccttat acttcttatt atcaatctta gcatcaccag
     7141 ctgtcacaat taaaattgca tcatctccta ttttaaatga gagtgtctta gctattcttg
     7201 ctggaataac cccagctgct tcagctgcaa gttccacagt agcactagat tgttcaaatt
     7261 ctaatatact gtcttccttg ccaaattgtt taaaatactc tcttacatta cttattgaca
     7321 ttaaataccc ctcctagtct gaacttttat aaatttcata ttattcttaa ttatatctat
     7381 aattgattta tttttcaaat tattccataa tattagcatt ccatgtatca ttattttttc
     7441 caactactag ataaagtcct tttgccattg gaatattgac aatatattct cctttatact
     7501 ctacactcat accagattca ttaaagtacc tataatcaaa atttagtcct tttggaatta
     7561 caaaatcaac ataaccttga ttatttggtt ttattccata tttcagagtt ctttcagtta
     7621 tagaattttt ttcattaaat aaatctacat ttacatatgc agaacactta ttcattccct
     7681 ttttattttc cacacaattt tcaaacttca ttttagcact gtttaattta taactatatt
     7741 tactattatt gcttatttta aatcttacaa tagtaccaac atctctactt ctcctgctat
     7801 caaaatcaaa aacaatttcc tctaaatgat ttatttcata atctttgaca cttaccatat
     7861 ctctatatac taatttattt atagctatga atagtgtaaa aatccccaaa ataaaaaatg
     7921 aaacttttat aaacttcttc atttttagtc tccttatatt ttgtgctatt gtgctataat
     7981 attaattaaa attttaataa ttaaacttat tcttttttga aatcttaata gaaaaataca
     8041 ttgatattat aaaaattatt attgttaaaa atgtcatact atataaattt atatacttag
     8101 tattagctat atcagttaca taatttgaaa gatatactcc tccaacttta ctataattaa
     8161 aatgctttac ttgatataca atcatactaa tcatcttcaa aagacttcca tatacaaagt
     8221 aaattaaaat acttactata aaaccacaaa ctttcatttt tccatatcca aacttaaaaa
     8281 ttaaaggcag taaaatcgtc aaaattgcta aaaatatatt tacagaaaaa aatacatcat
     8341 ttaaaaccat atttctaaag aacactagtg atgtcaattt acttagtaag gacacaatta
     8401 ttgttacaga aattattaat cctacaccta ataaatattt tgagtaaact atatcttcta
     8461 tactaactgg catactcctt ataaaaatcc ttgaatcatt ttcataatca cactcaaacg
     8521 tatttataaa tatcaaataa ctaacaacaa tacttactgt ataatatgaa attgaattaa
     8581 ggaaaaaata aaacacaaaa aatagcaata tgtactttaa attacggata ccatctgaac
     8641 taatccctat tattaaatct tttcttatta aattgaacat catctcacct catttacata
     8701 ataatacatt agttcttcta tagtaggatt tttgacctta tgcttatcaa tttctatcaa
     8761 taggcttttt actttcaagc tatctccaac tttaacaagt gcctcattat aatatttata
     8821 ctctttcaca ccaactatgc tttctttaat tgcttctaac tctgctttat ttgccctaat
     8881 tatcttatat tcttgttcta ttaattcttt cttaacagta aaaatctgat ttcctctatt
     8941 tataaaaaca agataatcag caattttaat taaattatca gtattatgag tagaaattat
     9001 tacactacta cttccatttt tcacaaaatc ttgtaagtac tgcattattt cagttcttac
     9061 aattgggtct atcccagcag ttggttcatc caaaatcaat aacttagcat tgtgactaag
     9121 tgcacttgat aacataagct tttcagattg acctttagat aaattttcaa tatgcatatt
     9181 ttcttctata tcaaacttat taaggtattc atcaaacctt tctctatcaa aattcttata
     9241 aaataaagaa actatttttc taaattcctt aacctttaaa tgcggataaa attgtaaaga
     9301 atcatataca aaaccaatat tgtttttaaa gcttattgga gaattttcaa tagtttcatt
     9361 acaaagttca atttcacctt catctgcatc aataatcccc attataattt ttattaaagt
     9421 cgtttttccc gaaccatttg gtcctataat cccaacaata tccccttcct ttaactctaa
     9481 gtttatatta tttaacttga attttcctaa tttttttgtg atattcctta tgctaaacac
     9541 atcttcacct cttatgcatt tttttatacc cacctataca tattttaaca cattcattac
     9601 aaaggtcatt tataaattta taaattaaga attataaaac tatgttataa tacaaagtat
     9661 gaagaggtga atatatgaaa agaattataa ctataaatac aaaccaatta aataagtccc
     9721 ttgagactaa agaactctta actagaaaac ttatcaatgc tggctttgaa gtgtattcag
     9781 atatttatcc agacacagag cttattatat caattggtgg tgatggctct tttttaagaa
     9841 cagttagaga tttcgatttt cctgagattc ccataatggg aataaataca ggtcatctgg
     9901 ggttttttcc tgatatttta ccagataaga ttgatagctt tattgaagct tatacaaaaa
     9961 aagattatat tattcaagaa atgtcacttc ttaatgcgga agtatataca actactagtg
    10021 gttctaatat gcttgctgta aatgaggttg ttataagagg agacaagtct aggactatac
    10081 acctaaattt aagccttgat aataaacata tacaaaattt tagtggagat ggtatgatta
    10141 tttctacttc cacaggttca acagcctata actattctgc tggaggtagt attgttgata
    10201 taaatcttga acttatgcag attacacctt tacatccgat aaataccaat gcatataggt
    10261 gctttacatc aagtataata tgttcaaatg aatctgttat aaagattgca ccagaatata
    10321 gatttgaaga ttctgtattg attgtcgttg atggagttga gcacagattt agacaaatcg
    10381 aaaatataaa agttagtata tctgatgcaa aaataaaatt acttagaatg tcaaactatg
    10441 agttttggca tagagtatct gaaaaatttt tatagactgt acttaggagg atttttaata
    10501 tgaaaatagg aataatgagt gatactcatg gtagcttact ttactttgaa aaagcgttaa
    10561 atgtattatc tgattgtgat gttttattac atggtggaga tgtcttatat catggaccta
    10621 gaaatgatat tccagaagga tataatccta aaaaatttat tgagacatta aataaattag
    10681 aaaacattgt gattgtaaaa gggaactgtg atgcagatgt tgaccagatg gttatagaac
    10741 atcctattca gagtccttat gtcatgagcc aatttggtga aattagaatt atattgaatc
    10801 acggttatat agaatcagaa gaagaaatta ttgacaaagc aaagaaaatg ggtggtgaca
    10861 tattagtttt aggacatact catgtaaaaa aactatacat ggatgataat ttaatagtaa
    10921 taaatccagg aagtacttct atcccaaaag atggaagcca ttctgtcgca attatagata
    10981 tcataaaaac tgatgatgag gatgagcttg aacttgatat aaacttaatt gacatcaata
    11041 ctggaaatat aataaatatc aatgattagt aaatttttaa taaaaataaa ataagcttta
    11101 atttattaaa ctcataataa aaacaagtaa accattttat ttctatctca attagaagtt
    11161 taagtacaat accttaaact tctaattaaa tagaatctgg tttacttgtt ttattatatg
    11221 ctatattgaa atataactta gtttacttg
//
LOCUS       DACXMZ010000125        11051 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.126, whole genome shotgun sequence.
ACCESSION   DACXMZ010000125 DACXMZ010000000
VERSION     DACXMZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.126"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..402
                     /locus_tag="JD016_12505"
     CDS             <1..402
                     /locus_tag="JD016_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HAU5000456.1"
                     /translation="NFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELIK
                     LKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNKR
                     PNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            471..1172
                     /locus_tag="JD016_12510"
     CDS             471..1172
                     /locus_tag="JD016_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAU5000457.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            1234..2559
                     /gene="rsmB"
                     /locus_tag="JD016_12515"
     CDS             1234..2559
                     /gene="rsmB"
                     /locus_tag="JD016_12515"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAU5000458.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            2567..3598
                     /gene="rlmN"
                     /locus_tag="JD016_12520"
     CDS             2567..3598
                     /gene="rlmN"
                     /locus_tag="JD016_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAU5000459.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            3618..4370
                     /locus_tag="JD016_12525"
     CDS             3618..4370
                     /locus_tag="JD016_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAU5000460.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            4387..6402
                     /gene="pknB"
                     /locus_tag="JD016_12530"
     CDS             4387..6402
                     /gene="pknB"
                     /locus_tag="JD016_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAU5000461.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            6510..7247
                     /locus_tag="JD016_12535"
     CDS             6510..7247
                     /locus_tag="JD016_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAU5000462.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            7345..8229
                     /gene="rsgA"
                     /locus_tag="JD016_12540"
     CDS             7345..8229
                     /gene="rsgA"
                     /locus_tag="JD016_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAU5000463.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            8234..8905
                     /locus_tag="JD016_12545"
     CDS             8234..8905
                     /locus_tag="JD016_12545"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAU5000464.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            8914..9555
                     /locus_tag="JD016_12550"
     CDS             8914..9555
                     /locus_tag="JD016_12550"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAU5000465.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            9686..10315
                     /locus_tag="JD016_12555"
     CDS             9686..10315
                     /locus_tag="JD016_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAU5000466.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            10370..11026
                     /locus_tag="JD016_12560"
     CDS             10370..11026
                     /locus_tag="JD016_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HAU5000467.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT         4374 a         1135 c         2174 g         3368 t
ORIGIN      
        1 aattttaatc cagaggatat aataatttta atacctcatt gtattcaaga gaatagttgt
       61 aaattaaaag ttactaatga tatagataat tgtaaagaat gtggaagatg caacatagga
      121 gaattaatta agctaaagga aaaaactaat gtaaaaatat ttgtagctac aggaggaacc
      181 ctagctagaa aaattataat ggacactaaa cctaaagctg ttgtagctgt tgcatgtgaa
      241 agagatttga catctggaat acaagatatt aaaaaaattc ctgttttagg tgtatttaac
      301 aaaagaccta atggaccttg tgtaaatact aatgtagaca tgatggatat tgaaaaagct
      361 ataggctttt taacaggaaa aaatatatta gtttgtaatt aaactgtttt taagttaagt
      421 gaatattaag aggatgtata ataaaaatat attttaggag agtgttattt atgtaccctg
      481 tatatggcgg tttttgggga tttgacccaa caatggttgt attaattcca gctatattac
      541 ttactatata tgcacaattt aaagtgagtt caacaacaaa taagtattta agagtaaata
      601 cacgtcgagg atatactgga gagcaaacag caagaagagt acttgattca aatggtttgt
      661 atgatgtaaa gatagaaatg gttagagggc atctaagtga ccattatgac ccaagaagaa
      721 aggctgtaag gttgtcagaa gatgtatatt atggaacatc tataacttct gttgcagtag
      781 ctgcacatga gtgtggtcat gccatacaac atgctaaagg ttatgcacct cttcaaataa
      841 gaagtagttt agtaccagta gtgaattttg cttcaagtat atcttggttt ttaatatttt
      901 taggatttat tatggctggt ccatttttaa aaattggtat actattgttt tcggcttcag
      961 tgttattcca aataataaca ttgcctgtag aatttaatgc ttcaagtaga gcaatcgttc
     1021 aacttgggaa tttagggatt atagatgaaa gtgagtcaag acaaagtaga agagtacttt
     1081 cagcagcagc tttaacatat gttgcagcag cattggtttc aatacttcaa ttacttagat
     1141 tgttactaat agctcaaaga agaaatgact aaaagtaaag tgggttgttt agaaacaacc
     1201 cttttttatg tttaataaaa gggagaaatt tatatggatg caagagaagt tggatttaaa
     1261 gtattatgtg atatagaaaa aaatgacaat tattcaaata ttgcaataaa taagcatttt
     1321 aaaaatttag aaataagtga tatggataga ggacttgcaa cagagttaat ttatggagta
     1381 atagaaaata aatattattt agattatata atcaataaac tttcaaaaat aaaggtaaaa
     1441 aaaatgtcaa cttatgttaa aatcttttta agaatgggaa catatcagat tttattttta
     1501 gatagtatat cagattatgc agcagttaat gaaactgtaa aattatctaa gaaatatgat
     1561 aaaaaatctt ctggtttcat aaatgctatt cttagaaatg aaataagagc taaagagact
     1621 ataatggaca taactgagga agatagtgta aaatacttat ctataaagta ttcctataac
     1681 tcttggatta taaaaaactg gatagataaa tttggacaag aatttgctga agatttgttg
     1741 gaagcaaata atgaaaagcc tagtatctat ataagaacga atacacttaa aataagtaga
     1801 gaagaactta ttgaaaaatt aagtgaaatg ggtattgtat gtttaaaagt tcctatggta
     1861 gaagaagcta ttaaagtcga aaaaatgaaa aatatagaaa aaaatgaatt atttaaggct
     1921 ggtttattta caattcaaga tataagttct atgatagtag ggaaagtaat taatccaaaa
     1981 gaaggttcat ttgttttgga tgtatgtagt gctcctggag ggaaatctac tcacttagct
     2041 actttaatga acaatactgg tcaagtagta gctagagata tttttgagca taaactaaag
     2101 cttataaact ctactgtaaa tagattgggg cttaaaaatg tgtttgtaga aagatttgat
     2161 gcttctgaga ttgatgaaaa tagtataagt aaatttgatt atgttttagc agatgttcct
     2221 tgctctggtt ttggtataat aagacgtaaa ccagaaatta agtataagaa agaagaagag
     2281 ttaaaagaca tcacttctat acaaaagaaa attttagaaa atgcatctaa atatgtcaaa
     2341 gttggtggaa ctttagtata tagtacatgt acaattcaag atgtggagaa tataaatata
     2401 gttacttctt ttgttgaaga aaatcacaac tttgaatttg ttccaattga tacagtaaat
     2461 gtagacttag ataatcaaga taaaggatat ttaaagatat atcctaatat tcatggtata
     2521 gatggttttt tcatagcaaa attaaagaga ataaggtagt gagagaatgg aaaagaaaaa
     2581 gatagtatta aaaaatttta ctgaagatga acttaaagaa tttatgaaaa ctatagatga
     2641 aaagcccttt agaggaagtc aaatattctc ttggatatat aagggtgcaa aaacttttga
     2701 tgatatgaac aatataccaa aaagtttgag aaataaatta gaagaggttt catgtatagg
     2761 gcatatagat atagaattaa agttagaatc taaggtagat aatacaaaaa aatatttgtt
     2821 tttattagat gatggaaata taatagaaac tgtgatgatg gattatgata gtagagttac
     2881 tgtctgtgtt tctaaccaag taggttgtag gatgggatgt aatttttgtg catctacaat
     2941 ggatggatta attagaaact tagagccatg ggaaatttta gaccaagtta ttaaaattca
     3001 agaagatact ggtaagaggg tatcaaatct tgtattgatg gggagtggtg agccacttga
     3061 taactttgaa aatacaaaac aatttttaaa aataattaac gaaaaaaatg gtcttaatat
     3121 agggtataga catataactc tttcaacttg tggaatagta cctaaaatgt atgagttggc
     3181 agacttagaa atagctataa atttagcttt atcacttcat tcaccatatg atgaagaacg
     3241 aagaaaaatt atgcctgtag caaatgctta ttctattgaa gaaatattga atgcatgtag
     3301 atattatata aaaaagacaa atagaagggt tacatttgaa tattctctta taaaaggggt
     3361 aaatgactca gaaaaagaag caaaggcatt agctaagctc ctaaaaggta tgctatgtca
     3421 tgtaaattta attccaataa ataaagttga agaaagagaa tacgaaaaac cagataaagc
     3481 atttatttat aaatttaggg atagtttgga gaagaacaat atacctgcca cagttaggat
     3541 gtctatgggt tctgatatta gtggagcttg tgggcaattg agaagaaaat acaagtaatt
     3601 tgtaaggagg aagtcgtatg gtttatagtt gtgcctccca tataggaaaa ataagaaaaa
     3661 acaatgaaga ctattgtgag ggagaagtta tagatacaga acatggtcca ataggaatat
     3721 ttgccatagc tgatggaatg ggtggacata aaaaaggtga agtcgccagt aaattagcag
     3781 tagaaaatat aattgatttt ctaaaggaaa acttattaca acatgataat gtaaaaatag
     3841 actatataga tgatatatta aaacaggctt ataataatgt aaactcaatt gtgcacaaaa
     3901 aatctatgga agatatagaa tttgaaggaa tgggaactac attagttacg gctatagtgt
     3961 ataataatgt cttatatgtg gcaaatgttg gagatagcag atgttatttg ttaacagatg
     4021 aaaaatttga taaaataact atagaccatt ccgtggttga agaacttatg atggcccatg
     4081 tcattacaga agaagaggca agaagacatc cacagaggaa tagaataact agagcaattg
     4141 gaacagatga tatggttgtt gtagatattt ttaagaaaga aatcaaaaaa agtgatataa
     4201 tacttcttgc gacagatgga ttaactgggt ttattgatga tgaagatata agagatttaa
     4261 tattagatta tgaatatgag agaactagta atataagtga agaattgata agtatggcca
     4321 atgatgtttc tggtaaagat aatgtttcgg ttatagtgat aaaagtataa ttaggacggt
     4381 gatattgtgg gagatacaat tttaggaaat cgatatgaaa tcatcaggaa gattggtgat
     4441 ggaggaatgg cctttgtata tgaggctaaa gacagattat taaacaggac tgttgcactt
     4501 aaagtactta gacctgaatt tgtagatgat gatgaatttc taacaaagtt taaaagagag
     4561 gcagaagcag tagcaagtct ttcacatcca aatatagtaa atgtttatga tgttggtgaa
     4621 gatggaaaag ttcactacat tgtaatggag tttgtagatg ggaagaatct aaaagagatt
     4681 atacaagatg aaggtatatt agatgaatat actgcattag atataacaaa acaaatagct
     4741 atggcactta gtgcagcgca caaaaagggg attatacata gagatataaa acctcataat
     4801 attctcatat caaatgaagg aagagtagta aaagtggctg attttggtat agccaaagct
     4861 gtttcaaatt caacaatgac taatattggt agtataatag gttcagtgca ttatttttca
     4921 ccagagcaag ctaaaggtaa atttgtaact aataatgctg acctgtattc tctaggtata
     4981 gttttatatg aaatgttgat aggaaaagtt ccatttaggg gagatagtcc aatttctata
     5041 gcacttcaac acattaatga tgatatagac tttacatcag aagaaaaagt taggattcct
     5101 caaagtgtaa gaactacaat aaaaaagctt acagaaaaat ctagtgcaga tagatatcaa
     5161 actgctgaag aattgataga agatatagag tatatagaaa aaaatattga tttagatttt
     5221 ataaaagaat atgatgattt tgcaactaag aaaattgatg aaaaagaaat taataaagtt
     5281 gtaaatccaa cacttgcaaa accagctcct gaaaaagtgg ttaagcctgt tgaagtagct
     5341 gatttggatg atgatgaaga ttactatgat gatttttatg aagaagatga tgatgaagat
     5401 gaagaagagg aagagataat gagagctaaa aagaatcaga gacctaagag tactccaagt
     5461 aagagaacta agaaaaagaa gaaaaaacag gaaagcccaa aatctagaag aagattaaaa
     5521 gtaattgcgg ctgttttgat actgatattg tgtgctcaag tattcttagc atataagttt
     5581 ttatttgctg gagggtttgg caataaatct ttgactgttc ctaatcttgt aaatatgaca
     5641 ttagaagaag ctcaaagtgc tgttgaaaaa gaaggattat acctgagtgt aaaaagtgaa
     5701 gagtataata gtgaagttga tgaaaattgt ataatatcac agacacctga aggtggaagt
     5761 acaaatgtca aaaagggtga tactataaat gttgtcgtaa gtaaaggttc aagtcaggca
     5821 tctgttccta atgtagtggg acttactttg agtaatgcaa aacaacttat tgaagaaaat
     5881 aacttaaaag tgggaactgt taagtatgaa tacagctcaa tatataaaga aggtacagtt
     5941 ttaagtcaga gtccgggtgc tggctcatca agagctcagg aaggtgatga ggtaaaccta
     6001 tatgtaagca aaggttcaga aaaatcaaat acacagactc caacagtacc agataaaaaa
     6061 ccaacaacac cagagaataa tacaccaaca gaacctggtt caaactcagg aaacagtggt
     6121 ggaaattcag gaggctcaaa ttcaggaaat agtggtggaa actcaggcaa taatggaagt
     6181 aactcaggag gttcaaactc aggaaatagt ggtggaaact caggaggctc aaactcagga
     6241 gataatggag gaagttcagg aggctcaaac tcaggagaca atggaggaag ttcaggaggt
     6301 tcaaactcag gagacaatgg agatagttca ggaggctcaa actcaggaga taatggagga
     6361 acttcatcag gaactggcgc aaacattggt aaaacagaat aaatattaat aaaataagag
     6421 ctgtcctttt atagaaaata ttttctttaa agggcagttt gttttgtata aaatactgaa
     6481 ttggttataa tataaggaga aaagtattta tgaagaagaa aacagtttta attacaggag
     6541 gagctagagg gattggtaaa gctatgtcta aagcttttgc aaaagaaggt tacaatgtat
     6601 tagtaaattt taataagtca gaaaatgaag ctaaagagtt gtacactata ttaaatgaaa
     6661 aaaatttttc tgtaaaactt tttaaggctg atatttcaaa tagagaagat gtagaggata
     6721 tggtagatta ttgtataaaa gaatttggag gattggatgt acttgtaaac aatgctggtg
     6781 taagtcaaga taagttgttt acagatataa ctgatgaaga ttgggataat atgatgaata
     6841 ttaatttaaa aggaagcttt tattgttcac aagttgcttt aaaatacatg atatcagaaa
     6901 aaaaaggaaa tataattaat atatcttcaa tctggggtat atcaggtgcg tcttgtgaag
     6961 tgcattattc tataacaaaa gcaggcataa taggtatgac aaaggcatta gctaaagaag
     7021 taggaccatc aaatattaga gtaaatagca tagctccagg tgtcattaat actgacatgt
     7081 tatctggata taatgaggaa gatatagacg ctcttgtgga agaaacccct ctaatgaggc
     7141 taggaactcc agaagatata gcaaattgtg ctattttctt ggcatcagac aaatcaaatt
     7201 ttatcactgg tcaggtaatt agtccaaacg gaggatttgt tatttaaaaa aaatgtggta
     7261 ttatataaaa ataggaaaga ataacacgat atactactca taaacagtat gtgtttatat
     7321 aaattttata aagggaggca tttgatgcta gaaggaaaaa ttataaaggg gattagtgga
     7381 ttttattatg tagatactta taatggtatt tatgaatgta aggctagagg gatatttaga
     7441 aagcagaaaa taacaccttt ggttggtgat agagtcaaaa taagcattgt tgatgaaaat
     7501 gaaaaaaagg gtatcttaga agaaatcgac agtagagata cagaacttat aagacctcct
     7561 attgcaaatg tagataaagc attaatagta ttcgcaataa aaaatcctaa accaaactta
     7621 tctcttttag atagatttat agttttggca gaaaaagaaa atttagaaac tgtaataatc
     7681 cttacaaaag cagatttaga tgataatgat acattagaaa ctgttaaaaa tatatatgaa
     7741 cttagtggct ataaagtcat tcctgtaagc aatattacta agttaaatat agataaagta
     7801 aaagaagaat taaaagaaaa tgtagtagtg tttgcaggac catctggagt tggaaaatca
     7861 agcttattaa atgaaataga tgaaaatttt aaacttcaaa caggagttgt aagtgataaa
     7921 ataaaaaggg gaaaacatac tactcgtcac gctgaacttt taaaattaga atttggtgga
     7981 atggttgcag atacaccagg ttttagttcc cttgcccttg aagatataga ggaagttgaa
     8041 cttaaagatt attttataga atttgataaa tttaatgatt gtaagtttgg ttcaaagtgt
     8101 attcatgaaa atgaacctaa ttgtgctata aaagaagctg ttacaaatgg agaaatatct
     8161 aaagaaagat atgatagtta tatacagctg ctacatgaaa taagacaaaa taatagtagg
     8221 aggtattaga aacatgatta aattagcacc atcaatatta tcagcagatt ttgcaaaatt
     8281 attagaagat gtaagaaaag tagaaagtgc aggatgtgaa tatcttcata tcgatgtaat
     8341 ggatggacac tttgtaccaa acattacatt aggaccactt gtagtcaaaa gtttaaagaa
     8401 agaaaatata aatatggtat ttgatgctca tcttatgata gaaaatccag accagtatat
     8461 tgaagaattt gtaaaagcag gatgtgatat aataactgtt caccaggagg cttgtgttca
     8521 tctacataga actatacaaa atataaaatc acatggaata aaagctggtg tagtacttaa
     8581 tccagctaca ccagtagata cgattaaaca tgtactacct gatttagata tggtattatt
     8641 aatgtctgtt aaccctggat ttggaggaca atcatttatt ccttgtgttt tagataagat
     8701 taaagagtta aaagctatta tagatagtca aggattaaac attgatatag aagttgatgg
     8761 tggtataagc cctaaaaacg tagccgaagt tgtacaagca ggagctaatg taatagtagc
     8821 aggttctgca atatttggaa gtgatgacat tcaagaaaca gttaatttat ttagaaagaa
     8881 tgcttctctt gaagaattag tttaaattta ataatgaaaa tctgtattgt tttaaatggc
     8941 gaaattgaag attacgaagt tacaagagat attataatta aggaatgtta tgactgcata
     9001 atatgtgcag atggaggggc aaatcacact tataaaatgg aaataatgcc agattatata
     9061 ttaggagatt tagattcagt agaagaagaa aagataaact tctataaaaa taaaggtgtt
     9121 aaatttgaaa aatttccatc taaaaaagat gagactgata cagagctttg tcttttttta
     9181 gctaagacac ttaaagcaaa tcatattgat ttttttggtg ctttaggggg aaggattgac
     9241 cacactcttg caaatataaa gctattatat tatttaaaag aagatggtat atattctagg
     9301 atactttctg ataaagaaga gatgtatata gttgaaaatg aggaaatttc attgtatgga
     9361 aacccagggg acactatttc tgtaatagct ataaatggtg atgctaaggg tgtaacactt
     9421 actggcttag aataccctct cgataattat tatatgaaat actctgtacc tattggaata
     9481 tcaaatgtta tgttgagtaa ttcttgtaag attaaagtag agcaaggctg tgtacttgta
     9541 gttagaaatt tataaaattt gattttgaca attggtttta tattgtagag cagatacaat
     9601 caagtaaaat atatttatga ttgtatctgc tttttagtat ataattataa atatataata
     9661 aactttgaca taagggggat tttttgtggt agatagtatt atttttgatt tagatggaac
     9721 attgtgggat tcaactgaag aagtatgcaa agtctggcag gatgttttaa gtgagcataa
     9781 ggaaatagaa ttaagtgtca caaaagattt atttagaagt cttatggggc tttcttttga
     9841 ggaaatagca aatcgtttat ttccaaattt aagtgaagaa gaaagaatga atttattgaa
     9901 tgaatgttct ttgaaagaat gtgactattt atctgaaaat ggagctaaat tatttgatga
     9961 tatagaagat actcttaaat tactatctaa aaaatataaa ttatttatag taagtaattg
    10021 tcaagctgga tatatagaat cttttttaaa agcacataaa ctagaacagt attttataga
    10081 ttttgaatgt cctggtaata ctggtttgca taaaggagaa aataataaac ttataattga
    10141 aagaaataaa ttaataaatc caatctatgt tggtgatacg caaggtgatg caaatagtgc
    10201 taagtttgta ggtatacctt ttatatatgc caaatatggg tttgggaatg tagatgtata
    10261 tgattatttt atagattcat ttaaagattt gttagaacat gatatattaa aataagatga
    10321 gtttaaaaat tatattaaaa taataaaaaa gttatggggg gatttaaaaa tggaattaca
    10381 agatactatt tttaaaagac aaagtgtaag aaagtttaaa aatcaagatg tatcagatga
    10441 agatatttta aaaatgataa aagcagcagg tgcagcacca tcaggcaaga atattcaaaa
    10501 ctggcatttt gttgttataa aacgccgtga tttaatggaa aagatagcag atgtgataac
    10561 taaaaagcaa caagaaatac ttgtagaaat ggataaagta tcagtggata aggctaatag
    10621 atttagaaaa tttgttaaaa attttacact attttactta aaagctccag ttttagtatt
    10681 agtttttaca aaggtatata atccatcagg atattatgaa ttagagctta tagatgcacc
    10741 taaggagact atagataaat tatttataag aaatccaggt atgcagagtc taggtgctgc
    10801 aattgaaaac tttacactat cagcaattga acttggatat ggttcttgtt ggttgacaag
    10861 tcaaaactat gcagcagatg agatagaagc tgttttagaa gcagaaactg gttttgaaaa
    10921 gggagagtat tttttaggtg caatgttagc tcttggagtg ccagaggaca atttgaagag
    10981 tccatctaaa aaaccagtag aagaaatatg tacatttata aaataaaagt caaggacatt
    11041 ttaaaatcga a
//
LOCUS       DACXMZ010000126        11006 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.127, whole genome shotgun sequence.
ACCESSION   DACXMZ010000126 DACXMZ010000000
VERSION     DACXMZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11006
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.127"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            269..3985
                     /gene="rpoB"
                     /locus_tag="JD016_12565"
     CDS             269..3985
                     /gene="rpoB"
                     /locus_tag="JD016_12565"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAU5000468.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            4027..7512
                     /gene="rpoC"
                     /locus_tag="JD016_12570"
     CDS             4027..7512
                     /gene="rpoC"
                     /locus_tag="JD016_12570"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAU5000469.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALEIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            7801..8223
                     /locus_tag="JD016_12575"
     CDS             7801..8223
                     /locus_tag="JD016_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAU5000470.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            8347..8817
                     /gene="rpsG"
                     /locus_tag="JD016_12580"
     CDS             8347..8817
                     /gene="rpsG"
                     /locus_tag="JD016_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAU5000471.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            8866..10932
                     /gene="fusA"
                     /locus_tag="JD016_12585"
     CDS             8866..10932
                     /gene="fusA"
                     /locus_tag="JD016_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAU5000472.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         4259 a         1401 c         2304 g         3042 t
ORIGIN      
        1 gaattataat taaatagggg ggatggtact tgtataaaat tttttataga aaattagacc
       61 tataaatgaa aaatatggat tttatattgg aaaattgtac ataatacata atatatgctt
      121 aaaataattt acaaaaaatc aaaaataatg catagtttac atataagata aaaaatatat
      181 tgaaaaatac ttgacattat gctaaaatta ttagatgcgt tagaatgaac tattgtttta
      241 tttttaatta ttgagaggtg aaaagtgaat gccacatcct gtcacgatag gtaaaagaac
      301 tagaatgagc ttttctaaaa ttaaggaaat agctgatgtg ccaaatctta tagaaattca
      361 agtagattcc tatgagtggt ttttaaaaga aggtttaaaa gaagtgtttg atgatatttc
      421 accaatagaa gattatactg gtaatcttat attggaattt gtcgactatt ctctagatga
      481 taaaccaaag tacgatatag aagagtgtaa agagagagat gctacatatt gtgcacctct
      541 aaaggttaag gtaagactta taaataaaga aacaggtgag ataaaagaac aagaagtgtt
      601 tatgggtgac ttccctttaa tgacagaaag aggaactttc gttataaatg gagcagaaag
      661 agttatagta agtcaattag ttagatctcc tggtgtttac tatgcggaag aaagagataa
      721 aacaggtaag agattgattt catctactgt aattcctaat agaggagcat ggttagaata
      781 tgaaacagat tctaatgatg taatatctgt aagagttgat agaacaagaa aacaaccagt
      841 tacagtttta cttagagctt taggaatagg aacagatgca gagataattg accttttagg
      901 agaagatgag agattatctg ctacattaga aaaagataat actaagacag tagaagaagg
      961 tcttgtagag atatacaaaa aattaagacc aggtgaacct cctactgtag aaagtgcatc
     1021 atctttatta aatgctttat tctttgaccc aaaaagatat gacctagcaa aagttggtag
     1081 atataaattc aataagaaac ttgctttatg ttacagaata atgaataaaa tttcagcaga
     1141 agacataata aatccagaaa ctggagaagt atttgtaaaa gctggagaaa aaataagcta
     1201 tgatttggct aaagccatac aaaatgcagg tataaatgta gtaaatctac taatggatga
     1261 tgataaaaaa gttagagtta taggaaataa ttttgtggat attaagtctc acattgattt
     1321 tgacatagat gatttaaaca taaaagaaaa agtacattat ccaactttaa aagaaatttt
     1381 agatggatat agtgatgaag aagaaataaa agaggctata aaatcaagaa ttaaagaact
     1441 tataccaaaa catatattat tagatgatat aatagcttca ataagttatg agttcaatat
     1501 attctataat ataggaaata ttgatgatat agatcaccta ggaaacagaa gaataagatc
     1561 tgtaggtgaa ttgttacaaa atcaagttag aattggtctt tcaagaatgg aaagagttat
     1621 aaaagaaaga atgacggttc aagatatgga agctataacg cctcaagcgt tagttaacat
     1681 aagaccagtt tcagctgcaa taaaagaatt ttttggtagt tctcagttat ctcagttcat
     1741 ggatcaaaca aatcctctat ctgagttaac gcataagaga agattatcag cccttgggcc
     1801 tggaggtctt tcaagagaaa gagctggatt cgaagtgcgt gacgttcacc attcacacta
     1861 tggtagaatg tgtccaatag agactccaga gggaccaaat atcggtctta taaactctct
     1921 aggaacttat gcaaaaataa atgaatttgg atttatagaa tcaccatata gaaaatttga
     1981 taaagaaaca tcaacagtta ctgatgaaat acattattta actgctgacg aagaagattt
     2041 attcgtaaga gctcaggcaa atgagccatt aacagaagat ggtaaattcg taaaccatag
     2101 agttgtttgt agaactgtaa atggtgctgt tgaaatggtt cctgaaagta gagtagatta
     2161 catggatata tctcctaagc aggttgtatc tgttgccact gctatgatac ctttcttaga
     2221 aaatgatgat gccaaccgtg cccttatggg agcgaacatg cagcgtcagg cagtgcctct
     2281 agttagaaga gaagcaccaa ttataggaac tggtatagaa tacagagcag caaaagactc
     2341 tggagcagtt gttgttgcta gaaattctgg tatagcagaa agagtaacag cagatgaaat
     2401 aattataaaa agagaagatg gaaacagaga tagatataat ctacttaagt ttaaacgttc
     2461 aaactcaggt acttgtataa atcaaacacc tataataaat aagggtgacc agataataaa
     2521 aggtgatgtt atagcagatg gtccagcaac tgatttagga gaagttgcac ttggaagaaa
     2581 ctgtcttata gcattcatga cttgggaagg ttataactac gaggatgcta tattaataaa
     2641 tgaaagatta gttaaagaag atagattatc aacaattcat atagaagaat atgaatgtga
     2701 agctagagat acaaaactag ggccagagga aataactaga gatataccta atgttggaga
     2761 tagtgcaatt aagaacttag atgacagagg tataataaga ataggtgcag aagtagactc
     2821 aggtgatata ttagttggta aagtaactcc aaaaggagaa actgaactta ctgctgagga
     2881 aagattactt cgtgcaatat ttggagaaaa ggctagagaa gttagagata cttcacttaa
     2941 agtgcctcat ggtgaatctg gtataatagt tgatgtaaaa gtatttacta gagaaaatgg
     3001 agatgattta tctccaggag taaatgaatt agtaagatgc tatatagcta aaaagagaaa
     3061 aataaaagtt ggagataaaa tggctggtcg tcatggtaat aaaggggtta tctcaagagt
     3121 attacctgaa gaagatatgc cattcatgga aaatggaaca ccactagaca taatacttaa
     3181 tccacaaggt ataccatcac gtatgaacat tggtcaggtt ttagaagttc atttaggact
     3241 tgctgctaag acattaggat ggtatgtagc aacatcagta ttcgatggtg ctaatgagta
     3301 tgacataatg gatgctcttg aagaagctgg gtatcctaga gatggtaaat taactttata
     3361 tgatggtaga acaggtgagt cttttgacaa cagaataact gttggatata tgtactactt
     3421 gaaactacat cacttagtag atgaaaaact acatgcaaga agtactggac catactcatt
     3481 agttactcaa cagccactag gtggtaaagc acaatttggt ggacaaagat ttggagagat
     3541 ggaggtttgg gctctggaag catatggagc tgctcacata cttcaagaaa tacttactgt
     3601 taagtctgat gacgttgtag gtcgtgttag aacatatgaa gctatagtta aaggtgaaaa
     3661 tatacctgaa ccaggaatcc cagaatcatt taaggttctt ataaaagaac ttcaaagttt
     3721 atgcttagat gtaaaagtat tgacagatga agaccaagaa atagaagtaa gagaatctgt
     3781 ggatgaagat gatacaatag gtgaatttga gctagatgtt gtaaatcata tgggagaagt
     3841 tgaagaaagt aatataatag aagaaattga agatgatttt gcagaaaatg cagaagatga
     3901 ggatatagag aatttagaag aattcactga agatgattta tttgaagaag aaatagattt
     3961 tgatagtgat gactttgata tgtaaattta attaatgtaa atctcaaata gaagggagag
     4021 aactccttgt ttgaattaaa caatttcgag tcgataaaaa tagcattggc ttctccagaa
     4081 aaaataagac aatggtctag gggagaagtt aaaaagccag aaactataaa ttaccgtact
     4141 ttaaaaccag aaaaagatgg tcttttctgt gaaagaatat ttggaccaca aaaagactgg
     4201 gagtgccact gtggtaaata tagaagagtt agatataaag gtgtagtttg tgatagatgt
     4261 ggagtagaag taactaaatc aaaagtaaga agagagagaa tgggacatat agagctagct
     4321 gctcctatgt ctcacatctg gtacttcaaa ggtataccaa gtagaatggg acttttactt
     4381 gatatgtcac caagatcgtt agaaaaaata ttatactttg cctcatatgt ggtagttgat
     4441 ccaggagaga ctggattaaa tgaaaaacaa ttgcttacag aaaaagaata cagaactgct
     4501 cttgaaaagt atggatatac ttttactgta ggaatgggtg ctgaagctgt aaagacatta
     4561 ctacaaaata tagatttaga gcaacaaagt aaagacctaa gagcggagtt aaaagatagt
     4621 acagggcaaa agaaagttag aacaataaga agattagaag ttgtagaggc atttaaaaag
     4681 tctggaaata aaccagaatg gatgatttta gatgcaatac cagtaatacc accagattta
     4741 agaccaatgg tacaacttga tggtggaaga tttgcgactt cagacctaaa tgatttatat
     4801 agaagagtta taaatagaaa caatagactt aaaagattat tagagcttgg agctccagat
     4861 ataattgtaa gaaatgaaaa aagaatgctt caagaagctg ttgatgcatt gatagataat
     4921 ggtagaagag gtagacctgt aacaggacct ggaaatagac cacttaaatc tttatcagat
     4981 atgttaaaag gtaagcaagg tcgtttccgt caaaacttac ttggtaagcg tgttgactac
     5041 tcaggacgtt ctgttatagt tgttggacca gaacttaaat tttatcaatg tggacttcca
     5101 aagaagatgg cattagaatt attcaagcca tttgttatgg ataaattagt taaagaaggt
     5161 tatgcacata atataaaaag tgcgaaatct atagtagaaa aagttaagcc agaagtttgg
     5221 gatgttttag aagatgttat aaaaagtcat ccagttcttc ttaaccgtgc accgactcta
     5281 catagattag gtatacaagc atttgaacca atcttagttg aaggtaaggc tataaaacta
     5341 catcctcttg tatgtacagc ttataatgca gactttgatg gtgaccaaat ggcagtccat
     5401 gtacctttat cagtagaagc acaagcagaa gcaagattct taatgctttc tgtaaataat
     5461 atacttgctc ctaaagatgg ttcacctata actactccat ctcaggatat ggttttaggt
     5521 tgctattatc taacaataga agcccaagat ggagctaaag gaacaggtat ggtatttaaa
     5581 gactttaatg aactattact tgcttattac aataaatcag ttcatctaca tgcattagtg
     5641 aaactgaagg taacacttga agatggaaga agttcattgg ttgaaagtac tgttggtaga
     5701 tttatattta atgaaaatat acctcaagac ttaggttttg tagatagaaa agaaaatcca
     5761 tttgcacttg aagttgattt cttagcagat aaaaaatctc ttggtaaaat aatagataaa
     5821 tgctttagaa aacacggaaa tacagaaaca gctgaattac tagattatat aaaagctcta
     5881 ggatttaaat attctacatt aggtggtata acagttgctg ttgatgatat gagtgtccca
     5941 gaagaaaaga aagtatttat agctgaggca gaagctaaag ttgataagta tgaaaaagca
     6001 tacagaagag gtctaatctc tgatgaagaa agatatgaaa aagttataga gacatggaca
     6061 gaaacaactg ataaagttac tgatgctctt atgggtggac tagatagatt aaataatata
     6121 tatataatgg cacattcagg agccagaggt tctaaaaacc aaattagaca gctggcaggt
     6181 atgcgtggtc ttatggcaaa tgcatctggt aaaacagttg agataccagt taaatctaat
     6241 ttccgtgaag gtttatcagt actagaatac tttacatctt cacatggtgc cagaaaaggt
     6301 cttgccgata cagctatacg tacagctgaa tctggatatt taacaagaag acttgttgat
     6361 gtaagtcaag atgttattgt aagggaaata gattgtggaa cagaagatac tacagagatt
     6421 tatgctataa aagaaggaaa tgaagttata gaagagatat atgatagaat tgtaggaaga
     6481 tatactatag accctatatt aaatcctgaa actggagaag ttatagttga ggctgattca
     6541 atgatacaag aagatgaagc agaaactata gtagctttag aaattgaaaa aattaggata
     6601 agaacagttc ttaactgtaa aactaatcat ggagtttgtt ctaagtgtta tggtagaaac
     6661 ttggcaacag gaaaagaagt taatataggt gaagcagttg gtataatagc agctcaatct
     6721 atcggtgagc cgggtactca gcttacaatg cgtacattcc atactggagg agttgcagga
     6781 gctgatataa ctcaaggtct accaagggtt gaagaattat ttgaagctag aaaaccaaaa
     6841 ggattagctg taataactga agtatctggt agagttgaaa ttgatgaaac aggaaagaga
     6901 aaagaagtaa atgtaatacc agaagaaggc gagactcaaa catatgtaat tccatatggt
     6961 tctagattaa aagttaagca aggtcaaatg ctagaggctg gagaccctct aacacaagga
     7021 tttataaatc ctcatgatat agtaagagta aatggtgtta agggagttca agaatatata
     7081 gttaaagaag ttcaaagagt gtatagactt caaggggttg atgttaatga taagcatata
     7141 gaagttatag taagacaaat gctatctaaa gtaaaagttg aagacccagg agatacagat
     7201 ttattgccag gtggatatga agatgtatta acattcaatg aatgtaataa agatgctata
     7261 gataaaggtc taagaccagc agttgctaaa agagttttac ttggtataac taaagcatct
     7321 cttgcaactg attcattctt atcagcagct tctttccaag aaacaacaag agtattaaca
     7381 gaggcagcaa taaaaggtaa agaagaccac ttaataggac ttaaagaaaa tgttatatta
     7441 ggtaagttaa taccagcagg aacaggaatg aagaaatata gaaatatagc tgttgaaaaa
     7501 attgaagatt aatagcatat agagtaaaga aatcttgaca accattattt gtggtgttaa
     7561 aatagtaaag tgtgttgttt aaaagtacac taagggcttg aacaagttct tagtgacttt
     7621 tagatggcaa taaatgagaa ctaaaaagca tcaagctaga aaagtgctaa tatcacatat
     7681 ttatgtgtta tagacattaa attgtaagta acgaatgtta caatcagtca gtatgaatat
     7741 tcatattgat gtagttggcg gtcactgata agatcgaaaa tttggaagga ggtgcagatg
     7801 atgccaacaa ttaaccaatt agttcgtaaa agtagaaaag cgctagagaa gaaatctact
     7861 gctccagcat tacaaaaagg ttacaactct ttaaataaga aagtaactga cgctagtgcc
     7921 ccacaaaaaa gaggggtttg tacttcagta aaaacagtta ctcctaagaa gcctaactca
     7981 gctttaagaa aagttgccag agttagatta actaatggta tagaagtttc tgcttatata
     8041 ccaggagaag gacacaactt acaagagcat agtgttgttc ttataagagg aggaagagta
     8101 aaagaccttc caggggttag ataccatata ctaagaggta ctctagatac agcaggtgtt
     8161 gataagagaa gacaatcaag atctaaatac ggtgcgaaga gacctaaaga agctaaaaag
     8221 taaaaacaat taatgattga ttatagttcg gtgctttaaa tatatatata tttacggcat
     8281 cacggcatta aatgatttat gtcgagtacc tatgatttaa gttaaataat taaggaggga
     8341 agcgaaatgc caagaaaagg taatattcca aaaagagaag ttttaccaga tcctatgtat
     8401 gggagtaagg tagttactaa attaataaat aatctaatgg tagacggaaa aaaaggaaag
     8461 tctcaaagaa tagtttatga tgctttcgca attgttgctg aaaagacagg agaagaagct
     8521 ttagaagtat ttaataaagc aatggataat ataatgcctg ttttagaagt aaaagcaaga
     8581 agagttggtg gggcaaacta tcaagtgcca atcgaagtta gacctgaaag aagacaaaca
     8641 ttaggtttaa gatggctagt aaaatatact agagctcgtg gagaaaaagg aatggttgaa
     8701 aagctagcta aagagataat ggacgcagct aacaacactg gagcttcagt taagaaaaaa
     8761 gaagatactc ataaaatggc agaagctaat aaagcatttg ctcattacag atggtaggat
     8821 atatagatat cttataaaat tgaggatatc ataaggagga taaccatggc tagaaagttt
     8881 cctttggaaa gaactaggaa tataggaatc atggctcata tagatgcagg aaaaactact
     8941 actacagaaa gaatcctgtt ttatactggg caaactcata aaataggaga aactcatgaa
     9001 ggagcttctc aaatggactg gatggagcaa gaaaaggaaa gaggtataac aataacttct
     9061 gccgctacta ctgcttcatg gaaagaccat agaataaaca taatagatac tcctggacac
     9121 gtggacttca cagttgaggt tgaaagatct ctaagagttc ttgacggttc agttgctgta
     9181 ttctgtgcaa aaggtggggt tgagcctcaa tcagaaaatg tatggagaca agctgaaact
     9241 tatggagtac ctagaatagc ttttgtaaat aaaatggata tattaggtgc agatttctac
     9301 aatgttgtaa gcatgatgaa atctagatta aattctaatg cagttcctat gcaattacca
     9361 ataggtaaag aagattcatt tataggtata atagacttat taaaaatgga tgctgttata
     9421 tacaaagacg acctaggggt tgaaatggaa gaaacagata taccagaaga tatgaaagaa
     9481 ttagctgctg agtggagaga aaaattagtt gaatcagtag ctgaaactga tgaagaatta
     9541 atgatgaaat accttgaagg tgaagaactt actatagatg aattaaaagt tgctataaga
     9601 aaagctacta tagcttgtga aatgaaccca gttttctgtg gtacagctta tagaaacaaa
     9661 ggtgttcagt tagtaataga tgcagtttta gattacttac cagcaccaac tgatatacca
     9721 gctataaaag gtatattagc agatggagaa gaagctgaaa gacattcttc tgatgaagag
     9781 ccattctctg cattagcatt caaaataatg actgacccat tcgttggaaa actagcgttc
     9841 ttcagagttt attctgggac attagagtca ggttcttatg ttttaaatgc aactaagaat
     9901 aagagagaaa gaattggtcg tatattacaa atgcatgcta atacaagaga agaaataact
     9961 aaagtgtatg ctggagatat agcagcagca gtaggattaa aagatactac tactggagac
    10021 acattatgtg accctgctaa tccaataata cttgaatcta tggaattccc tgagccagtt
    10081 atatctgttg ctatagaacc aagttctaaa gcagctcaag aaaaaatggg tatagctctt
    10141 caaaaattag ctgaagaaga cccaactttc acagttaaga ctgaccaaga aacaggacaa
    10201 actataatat ctggtatggg tgaattacac ttagagataa tagtagatag attattaaga
    10261 gaatttaaag tagaagctaa agttggtgca ccacaagttg catatagaga gactataact
    10321 caaccagttg atgttgaata caagtactct aagcaatcag gtggtagagg acaatacggt
    10381 cacgttaaga ttagagttgc tcctcaagag ccaggtgaag gatacaaatt cacaaacaaa
    10441 actgttggtg gatcagtacc aaaagaatat gttggtccag ttgacatggg tatacaagga
    10501 gctatgcaat ctggtatagt tgctggatat ccagttgtag acgttgctgt tgagttatat
    10561 gatggttctt accatgaggt tgactcttct gagatggcgt ttaagatggc tggttcaatg
    10621 gcattcaaag atgctatgaa gaaaggtaat gcagttcttt tagagcctta cttcaaagta
    10681 gaagttgtta ctccagaaga ttacatggga gatgttatgg gtgacttaaa ctcaagaaga
    10741 ggattaatcc aaggtatgga agctagaagt ggtgcacaag ttataaatgc attcgttcca
    10801 ctttcagaaa tgtttggata ctcaacagac ctaagatctt caactcaagg tcgtgcaact
    10861 tacacaatga tattcgatca ctatgagcaa gttccagctt cagttgctaa gaaaatagct
    10921 gaaggaaaat aatttaaaaa taattaataa tataaatata aaaaataatc agctagatga
    10981 gagatatctc tcatctagca caagat
//
LOCUS       DACXMZ010000127        10975 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.128, whole genome shotgun sequence.
ACCESSION   DACXMZ010000127 DACXMZ010000000
VERSION     DACXMZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10975
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.128"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(61..972)
                     /locus_tag="JD016_12590"
     CDS             complement(61..972)
                     /locus_tag="JD016_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAU5000473.1"
                     /translation="MDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRSYIQKIIKDGKVK
                     VNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDVLIINKPQDMVVH
                     PAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLMIAKNNYAHNFLA
                     EQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKDGKNAITHFEVVE
                     RFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKLKGQTLHAKKLGF
                     IHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(977..1423)
                     /locus_tag="JD016_12595"
     CDS             complement(977..1423)
                     /locus_tag="JD016_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAU5000474.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(1733..2512)
                     /locus_tag="JD016_12600"
     CDS             complement(1733..2512)
                     /locus_tag="JD016_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAU5000475.1"
                     /translation="MKKIIWGICICLLSIIGVYTYSHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(2567..3049)
                     /locus_tag="JD016_12605"
     CDS             complement(2567..3049)
                     /locus_tag="JD016_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAU5000476.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENILLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(3315..4736)
                     /locus_tag="JD016_12610"
     CDS             complement(3315..4736)
                     /locus_tag="JD016_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HAU5000477.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKQQNNHKLAS"
     gene            complement(4882..5652)
                     /locus_tag="JD016_12615"
     CDS             complement(4882..5652)
                     /locus_tag="JD016_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000478.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELSNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(5657..6859)
                     /locus_tag="JD016_12620"
     CDS             complement(5657..6859)
                     /locus_tag="JD016_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAU5000479.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DTGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
     gene            complement(7447..8448)
                     /gene="trpS"
                     /locus_tag="JD016_12625"
     CDS             complement(7447..8448)
                     /gene="trpS"
                     /locus_tag="JD016_12625"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAU5000480.1"
                     /translation="MSEKKVILSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(8471..9169)
                     /locus_tag="JD016_12630"
     CDS             complement(8471..9169)
                     /locus_tag="JD016_12630"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAU5000481.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     misc_binding    complement(9212..9452)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9543..10946)
                     /locus_tag="JD016_12635"
     CDS             complement(9543..10946)
                     /locus_tag="JD016_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAU5000482.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
BASE COUNT         3639 a         1950 c         1141 g         4245 t
ORIGIN      
        1 tcactaaatt tatttactta tataaaaaca aaaaatgatt tataagtatt atacaaatgg
       61 ttattttatc tttttaataa cttccttaaa atactcaggt aattctgaat taaattcgat
      121 atattcctta gtagtaggat gtataaatcc caacttctta gcgtgcaaag tttgtccttt
      181 taatttaaat ttagtttctt ttggaccata tacataatca cctaaaagtg ggtgatttat
      241 agacaaagca tgaactctta tttgatgagt tctaccagtt tcaagcctag ctctcatatg
      301 tgtaaactta tcaaatcttt ctacaacttc aaagtgagtg attgcatttt ttccatcttt
      361 tactacagcc atttttaatc tatccttagg atttctccct ataggtttat caacagttat
      421 tttatcttct ttaaccacac catagcatat aaattcatac tccctagtta tagtatgttc
      481 ctttaactgt tctgctagaa aattatgtgc ataattgttc ttagctatca taagaagacc
      541 agaagtatct ttgtctattc tatgaactat accaggtcta ataactccat ttattgatga
      601 caacttgtct ttacaatgat ataaaatccc gtttactaac gtattttcat agtttccagg
      661 agcaggatgg acaaccatat cctgaggttt atttattatt aagacatcat catcttcata
      721 tacaatatct attggaatat cttgaggtaa aacttctaat actttaggtt ctggtatctc
      781 tataacaata ttatcttcct ctttaaccag atatctagct ttttctactt tattattaac
      841 ctttactttt ccatctttta taattttttg tatataactt cttgacatat cacctaattg
      901 ttctgctaga taaatatcaa gtctaacttc ttcttcctct tcaagtacta aaaactcttt
      961 tacttcatcc attatatcac ctacttttac tttcaaaaaa taaaacatat atacataaaa
     1021 acacagttcc tacaactaca aatacatcag ctatattgaa tacatattcc catataattc
     1081 taaagtcgaa gtaatctact acaaaaccta atcgtactct atcaattaga tttcccaatg
     1141 caccagatat aattaatata attcccaacc ttccaaatat atgtactttc cttgtattaa
     1201 gatagtatag tccaaatact gttgcaagta atgcaacaat tataaatatc cattgattat
     1261 tttgtaataa tccaaatgct gcacctctat tttcgacata agttaaatga aatacattat
     1321 ttattattgg tattgttgat acatccacca aattattcaa tacccatatt ttagacagtt
     1381 ggtctaaacc tatgagtaga attattatta atatatatag caatgatttt tccccctata
     1441 aattatttaa aaaccaattt tatttgaagc acactattat tattatacaa aaaaaatgct
     1501 ctaaagagca actttattaa aattgtattt tcttaataaa gctagaacaa aatttaaaat
     1561 agtactactt tttttatatt gtagatttct acatatttaa aatcatatca ttatgtataa
     1621 aaaatttaaa aataccatac taatcaatat tttttgtgta ttttatagat taaagattaa
     1681 tgatgtctgt aatttaaact tacaatcacc actaatcttc aaacaaatat ttttactttt
     1741 gcacttgctt atttttatct aaatgttgta taagttcaga tactgttacg aatttatatc
     1801 ctctctcttt taatttagga attactactt ctaaagcttg tatagtttgt gatttatcat
     1861 ttcttttagt gttataatca tgtaaaagta ttatattacc attttggacc tttgtaagga
     1921 tagtatttat aattgcatct actccagggt tagaccaatc tctagggtct aagtttgacc
     1981 aaagaattat attcatgcct ttagaaacta taatatcaca gacactttta ctcatggctc
     2041 tatatggagg tctaaatatc tttggttcta ctcctattac actttttata atattttgcg
     2101 tatctgttat ttctttttca acactactat aactattttt tgcaacattt atatgtgtaa
     2161 atgtatgatt ccctatttca tgcccctcat catattgtct ttttaataat tctttgttat
     2221 attctacttt ttctccaact acaaaaaaag ttgcttttac atcatttttc tttaatatat
     2281 caagtacttg tggtgtaaaa tcctcatctg gaccatcatc aaaagtcaat gctaaaattt
     2341 tttcatcctc atttcctttt tttattatta catcttcata atcgtctttt atattgtcat
     2401 ttaaactata tgcttctttt gaactatctt gattatgtgt attatgacta tatgtatata
     2461 cacctattat agataataaa catatacata taccccatat tattttcttc atgtcattac
     2521 ctcttttcta attcattcta gcaatatatt ttataatgtc agttttttaa tctctcaata
     2581 ttccattttt atttaaatca gttaaatttg ttaggtcttc tgaataaaag ttagaatctt
     2641 ctacatcagg gttttgcatt gcatcaccta ataaaatatt ttctttcttt gcacaatcat
     2701 tacataaact tgtatctggc aatatatcca atctttctaa atctatttga ttatgacata
     2761 aatcacaaat cccatatgtt ccattctcta ttttagataa tgctaagtct atttgataca
     2821 atcttccctc ttcatgattt attagactgt tttgcatatt ttgcatatat aaatctgttc
     2881 ccatgtctcc tatatgatta tcataactag ataattctcc tgatgaatac ttttcacttg
     2941 tatgtttagt tgtatctcca aataaagtat tatctttcat atcctctact aaattagtta
     3001 aattttctct ttctttcaat aatttatctt tatatttttt agtatccatt tttaacacct
     3061 ctaattttaa tatttacatc ataaaaatag tatgtggaaa agtaaattaa acatacaata
     3121 aaaataagat ttgattcgaa aaatagaatg tctcaaaatg aaatttttag ttcatttaac
     3181 cctctttttt atataaaatc aaatatattt ttatcctttc agcaataaaa aagagactta
     3241 tctctatttt gagatagcct cttttttgaa cataatttaa atacaatttt ttaattttta
     3301 tagttattat tttactaact tgccaattta tgattatttt gttgcttaat atgctttgct
     3361 tttttaaaga ataaaagacc aagaattatt gctacaataa cacctattcc attagatata
     3421 ttaactggta gtctaaatcc ttcttttgct tgcattatat aactaaatgt tactacagtc
     3481 ataaaaagtg ctggtaatgt agttatataa tggtttttat ttgctttgac taaatatgca
     3541 gaaccagtcc acaacattat cattgcaagt gtttgatttg cccaagagaa atatctccaa
     3601 attatattaa aatctataaa tgtaagagca acaccaactg cgaataatgg taaagctatt
     3661 aaaaatctat ttttgactgc atcttgatta taattcattg catctgctat tgtcagccta
     3721 gcacttctaa atgctgtatc tccagaagtt ataggacatg ccacaactcc aagtatagct
     3781 aaagctcctc caataggccc cattaatcca acagatactg tatttactac tgttgctgct
     3841 ccaccatcat taaatatcac attaagttga ggtatgccat ggaaatatga catggctgct
     3901 gctgcccaaa ctaaagctat tataccttct gcaaccattg acccataaaa tactggtctt
     3961 gcctcactct ctttctcaac acatctcgcc ataagtggtg attgagttgc atgaaaacct
     4021 gatattgctc cacatgctat tgttataaat aagtatggga ataaaggtgt tccatttgga
     4081 ttcatatttt gtaaatttat atttggtatg ttatacccct ttattattaa tcctccagct
     4141 acacctactg ccataattaa taatgctgct ccaaataaag gatatatttt accaatcaat
     4201 ttatctattg gtaaaacagt agccataata taataaagta ttatcactcc aacccatatt
     4261 tgtgcgttta cacctgtcaa tgtctttaat aatcctgctg gacctgttac aaatacaact
     4321 ccaaccaata ctaaaagaat aacactaaat attctcattg ctatttttgc accatttcct
     4381 agataaaggc ctaccaactc tgatgcagat gtacctttgt ttttcattga tagatatcct
     4441 gaagcaaaat cgtgtactcc accagcaaag attgttccaa acacaatcca taaaaatgct
     4501 gatggtccaa ataatgcccc ttgtatagct ccaaatatag gacctaaccc tgctatattt
     4561 agaaactgaa ttaaaaagat tctcttccat ttcattggca cataatctac tccatcccta
     4621 caagctatag ctggagtttg atttttgtca ttgattccaa aaaccttttc tacaaaagta
     4681 ccatagacaa aataacctac tataagtact actattgaac ctaaaaagct taccataaat
     4741 tcatcccctt agtttttata taattgcttt ataaattttt atgagtttaa gaaaactatt
     4801 tccagttcaa atagtaatac ttttttatgc taatataaat attaattaca ttaagtgcat
     4861 atttaagtac atgaaatgac tttatatatg cattaacagc ctaaattctt ttactttgct
     4921 tctacttaca gttacttcat agtcactatt ttttaatttt aatatgtatg tattattaaa
     4981 ccatggtata atttctttta ctttggttaa gtttacgata taagacctat gtgttctaaa
     5041 aaatgtttta tcatttataa gattttcgac ttctgatatg ccttctttta atataaactt
     5101 ttctttctca gtatatataa atgtctgcct ttcattagct tcacagtaat atatatcatc
     5161 aatatctatt acaactaatt tatcaccttt ccaaaggcta attttatgtg ttatttcttc
     5221 tatctcctga tttactgctt ccttcttata tttgtataaa ttactattta cattagatag
     5281 ttcaatatca ctcatctcac ttttttctaa tttattcagc atagaaataa ttctctcttc
     5341 agaatatggt ttcaatatat aatcaaatac atccaaagaa aaagcatcta ctgcataact
     5401 ttcatatgct gttataaaaa caattttagg tcttgattta aactgattta gtgtttttgc
     5461 aagtagcatt ccatcaagat gaggtatatt tatatctaaa aaaattacat ctattttatt
     5521 ttcctgaata aagtctaaca cctctatacc attggtaaat tcacttacta cctctattcc
     5581 acttttattt tcaataaaat atctcaattc ctttcttgct ggaaattcat cttccactat
     5641 tatagctctc atacttctac tcctttacat aaaatactat caaagtccca ttatctattc
     5701 ttcttatatt aagccctctt ccatacatca gtttaagcct taaatgtaca ttataaagcc
     5761 caatcttatt ttctggcata ttatcttgat atacattatt tataatttcc tcacttatac
     5821 ctataccatt atcctctatt gatattctca cagtattttc taatgaatac ggtaactttt
     5881 ttatggatat tttcactaca ccagctctac cactttctaa aataccatgt attattgcat
     5941 tttccactaa tggttgtata atcaaacttg gaatctttat atttacatct tctatatcat
     6001 aagatacagt tagtaattct ccaaacctag ctttttctat ctctacaaaa gatttaactt
     6061 gttcaatctc tttatttata tcaattaagt tatcactata ttcaagatta tatcttagat
     6121 aagttgataa atttattatt aactctctag ccttatctgg actaaatctt ataaatgaag
     6181 ctattgtgtt tagggaatta aacaaaaaat gcggattaat ttgtctttga agtgctttta
     6241 tttctgcttt atttctcgct tctatatctt ttttatcttt ctcaatcccc tctactatag
     6301 atataacaaa tcctattcca atctgcccga ctattaaagg taaatatatt ccaccgatta
     6361 tttgaattgc taaagaatgt ggatatgata ttaagtatat aagtaatata gtaaaacttt
     6421 cagaaatcat gcctaccaat attccataca ttactctatg ttgttttggt atcctcttat
     6481 atagaaatcc tgataataca cctgctagta tacttgataa aagacatgga acagatgtta
     6541 tccccccagt atccataaaa tatctatgaa tccctgaaaa aactcctgct gtaataccta
     6601 ccattggacc aaataaaatg ccacctgata ctatagatat tattctagtg tttactattg
     6661 accccatata atttatacca ctataagttc caaatatggc taaaaaagta aaaactaatg
     6721 ctatacagac caagtcatta aaactatatt cttccttttg aaatatctgc ttaaatattt
     6781 ttaactttgt tattagaaga aacaatacta caataaggct tgtcttttct attaatgcga
     6841 ttaaaattga tgttttcata atcccccttt aaaaaataaa acattactta atttaagttt
     6901 attattctta taataaatta gatacataaa aataaattcc ataattattg tatcatatca
     6961 cctatatata aacataaatt catttaatta tataaattta aaaaatattt ttttgatata
     7021 ttattgacaa ttttcattat aagagttaaa attaactcat aaattacata ttgggtataa
     7081 aatacaatgt atgttagaag ttgtttcaga aggctatggt tttctgattt ttttatgctc
     7141 agtagtgata tgcaaaaaag acaaagttga catcagttaa cttttacctt tattctactt
     7201 tccccaaagt aagaataatc taaccccttt gagatgagca aaagctcatc tttttttatt
     7261 atcaaaagat gctgtctcaa aatagagata atcctctttt tcattgttga aatgattaaa
     7321 atatatttta tttcatacaa aaaagagtgc ctcatgaaca aaagttcact ttgagactct
     7381 ctctctcata tcatatacct ttttaataac tttaaaattt atttatcaag taaagatagc
     7441 taataattag tatcttctct ctataaatcc aactttttta taaacttttc taagagtttt
     7501 tctagcttgt ttttcggctt tttctgctcc catactatat attttttcta aataatcttt
     7561 attattaaga aggtcaacat atttttctct tataggtctt aaagcttctg atactacttc
     7621 agccacatct tctttaaata tgccataacc ctttccttca tacatgttta caatctcttc
     7681 tacactcttt ttacttaaat tagaatatat atcaattaga tttttaatac ctggttgttc
     7741 atcattgtat tttacaacac ctaaagaatc agtaacactt ctttttatct ttctctttat
     7801 agaatctgaa tcatctgcta atagtataaa tgcattttca tttgaatcgg atttactcat
     7861 ttttttagtt ggctcttgca aactcatcac tcttgctcca ccttttgggt agtatccttc
     7921 tggaactaca aaagtaggtg aaaatctatt attaaatcta tttgctaaat cacgagctaa
     7981 ttctaaatgt tgtttttggt catctccaac tggaactagg tcagtttgat acaacaatat
     8041 gtcagcagcc ataagaactg gatatgtgaa taaacctgca tttagatttg cttcactctt
     8101 ttgtgattta tctttgaatt gagtcattct acttaattca cccatataag tcatagtatt
     8161 caatacccac ataagttcaa catgctcttt gacatgtgat tgtataaata tagtattttt
     8221 ttctgggtct aatccacaag ctatatattg agcaagtaat tctatagtat tggctcttaa
     8281 aactttaggg tcttgaggta ctgttattgc atgcaaatca actatactat aataacaatc
     8341 ataattgtct tgaagttcag tccagttttt tatagctcct aaataatttc ctaaagtcat
     8401 tttgcctgat ggctgtgcac cacttaatat aacttttttt tcactcattt ttttgtacct
     8461 ccttatatat ttatattgat tttaacatat ttaaaactat gtctttagag ttattggcag
     8521 catcatgtac aaatacatca taagtaacat cagcacttcc atctgcctta tcagacattg
     8581 ctcttattat tacaaatgga gtattattta aatagcatac atgagctata gctccacctt
     8641 ccatttctcc acaatatcca ccaaaatcat ttactaattc ttcttttaat tctttagaat
     8701 ttataaattt atctcctgta actactcttc cttttaatac tttatgagtc tttacttctt
     8761 ccacagaaga tttgtaagca gcatctataa gtctttcatc tgctttaaat acagaagtct
     8821 tcattcttgg tataactccc ttagggtctc caaatgctgt agtgtctaca tcatactgta
     8881 tagcatcagt tgatattact atatcattaa catcaagttt ttcgtttaaa gctcctgcaa
     8941 cacctgtatt aacaatagca tcaaccttaa attcgcttat tagtatctgg gcacaaagag
     9001 cagagttaac cttacctatg ccacacttta ctaacactac attcttacct tccaaaattc
     9061 ccttataaaa ttctaagcta gcttttttta tagtctctct aatatccatt aaatcaacta
     9121 aaatactaac ttcttcatcc attgcaccta taattcctat tacgttcatt tttcctccta
     9181 aattttctat attaaaaaat ccgtcctaaa tcaataggac ggatatattt tccgtggtac
     9241 cacctaaatt tatatacact caaaattgaa tatatatatc ttaatatatg ctataacgtg
     9301 catacacgat atcaaatact ctgtaatctt aaattacatt tcattttact cctcaaagac
     9361 ccattcaata aatacttttt attaagttct cagcatcctt aattctctgt gcaaaaatat
     9421 atttatttac tatttcttat ctcaggattt aatttattaa tttctttatt ttataatatt
     9481 ccttacacta taaattgtca actatatatt ataatatcca aaagaaattt ttaaaataca
     9541 ttttatttag ctgttttctt ttcatatcta ttccataacc atagaccaga taatataact
     9601 agaagacctc caccaatcat ctctatctca tatataacat tttcagcaaa tgtaggcata
     9661 actggtgctg cagataataa tatacccact acagttacaa ctaaaaccat ttttgctaaa
     9721 cctaggcaag tacttgttga ttttgccata gtataaggtc taatttcatc tggtctctct
     9781 tttcttagtt taatataaga agcatataac aatacatatg ggaataatgc agttaatgct
     9841 gtcatagtta caagaacatt gtatatagca tcaacagaag gcattaatgt agtcaccaat
     9901 agtattatag taactattat tgcttgaagt ataactgctt tagaaggtat cttgtgttca
     9961 tttacctcta ctaaactatc tggaaatatt ccttttttaa cactaccaaa taacattttt
    10021 attggtgatg ctatgtaaag aactattgct cctaatactg aaaaagctat accaaaagct
    10081 attaattgaa ccaaccaaga accaattcct aaagacgcac aaacagcaga aagtgcatct
    10141 aatacacctt tagaagcagt aatttggtct ggtggtatta tcattgttat tgcaattgaa
    10201 cctaatacat ataatgaagc tactattcca gctgatatta atattgcctt agggaagttc
    10261 ttttcaggct tatctatttc tgttatgaag ttagcagcag tctctgctcc tgctaaacca
    10321 aacattatag ctgatatagc aacaaatgca tccatattaa gttttggagt cattgaagct
    10381 attgtgtatg tagaagctat atcatgtttt ttcaacacaa atactgctac aaatgaaaat
    10441 attataagta tagcacttgg tattgttgaa cctaatgcac ctgtattagt aaatattttt
    10501 gtaaaactta ttcctttagt tgctataaga gataatgccc agaatattat taaagataat
    10561 ataagtacaa acatcttatt atcagcaagg gctggatttc ctattgtata agaaacattt
    10621 actgctaaga aagttaaaaa tgaagaatac caaaatattt tagctgtcca gtttaaccaa
    10681 gaaactaaaa atccatactt ttctccaaag gcttccttaa cccaatcgta aagacctcca
    10741 tcaccttttt gatatgtaga agctaattca gcacaaatta aagctcctgg tacaaagtat
    10801 ataaatgaga ataataacca cataggtatt gcacctaatc ctaaaccaaa gcttgatgct
    10861 gtagatttag ctatccatct tataccatat aaagctgata cattcataaa taccaaactc
    10921 cataggccta tttttttctt ttccatccta atttaatttc ccctctctat taatt
//
LOCUS       DACXMZ010000128        10959 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.129, whole genome shotgun sequence.
ACCESSION   DACXMZ010000128 DACXMZ010000000
VERSION     DACXMZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.129"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            138..1151
                     /locus_tag="JD016_12640"
     CDS             138..1151
                     /locus_tag="JD016_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAU5000483.1"
                     /translation="MNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYIFISDKF
                     DDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNIRLINLS
                     LSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFTLDGDDS
                     FWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEKPGITIS
                     DLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCLDIEENN
                     EKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLTELVERY
                     KNL"
     gene            1284..2582
                     /locus_tag="JD016_12645"
     CDS             1284..2582
                     /locus_tag="JD016_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAU5000484.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRNLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(2696..2941)
                     /locus_tag="JD016_12650"
     CDS             complement(2696..2941)
                     /locus_tag="JD016_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HAU5000485.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3089..3955
                     /locus_tag="JD016_12655"
     CDS             3089..3955
                     /locus_tag="JD016_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HAU5000486.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(3959..4495)
                     /locus_tag="JD016_12660"
     CDS             complement(3959..4495)
                     /locus_tag="JD016_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HAU5000487.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(4686..4889)
                     /locus_tag="JD016_12665"
     CDS             complement(4686..4889)
                     /locus_tag="JD016_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HAU5000488.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5180..5944
                     /locus_tag="JD016_12670"
     CDS             5180..5944
                     /locus_tag="JD016_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAU5000489.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            complement(6062..6199)
                     /locus_tag="JD016_12675"
     CDS             complement(6062..6199)
                     /locus_tag="JD016_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000490.1"
                     /translation="MPTFKQTYTLWKYCLTAFINSSKFLNPSKKSQLLDTFLINLIYN
                     Y"
     gene            6333..7646
                     /gene="prdC"
                     /locus_tag="JD016_12680"
     CDS             6333..7646
                     /gene="prdC"
                     /locus_tag="JD016_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HAU5000491.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8049..8134
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            8716..10767
                     /locus_tag="JD016_12685"
     CDS             8716..10767
                     /locus_tag="JD016_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HAU5000492.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQIPDGEKLSDGQIPDGEKPS
                     DGQIPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINKIVYNFWED
                     EEDVKVDEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDIT
                     KTLTFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFN
                     GQENQNEEAVKSILYAGSEFDGFGYKQQFNLGEEENDIMTYSATQSAIWIILGQIDEQ
                     ERLKQYQGSIDLCDKLIEIAKTEEERAEYQKRKEVAINTKAYLEALLKAGREELKPND
                     TGKPSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLKLPDGVTAYDVDGNIIGTG
                     EVEISTQQKFKLKSVGKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKDHSSLLPSS
                     KQNLLSYTIEKKNGQEVNFNIGLPTDDDDVVNPPVPPIDDDVVNPPVPPTDDTNGHKP
                     KPSPPIDDTVINPPVPPMDDTIINPPVPPTEDAVLNPPVPPMEDAVLNPPVPPTDDTV
                     LNPPVPPVSDTVEKTPELSRDDTIVKSPKTGDETQLMSYVFISVIAICGLAYQCKIKR
                     N"
BASE COUNT         4423 a         1215 c         1898 g         3423 t
ORIGIN      
        1 tatatggtgt acttatttta tgtttttagc aagaaatata gcatttaaaa gataaattgt
       61 gcaacttaaa tcaaagttta agatgaacta ttaacataga tatattaaaa acttttgtaa
      121 tggagaatat atgtgatatg aataataaag ttaaggttgc agtattagat acaggaatag
      181 acaaagaaca tgattactta aaagataatt tagttggagg aattgctttt gaatgtatgc
      241 atgattatat ctttatatca gataaatttg atgatgaaga tggtcatgga actgcttgtg
      301 catcaataat aaagaaagag tatgaagatg tagagttatt tgtaataaaa attttgagaa
      361 aagatggtat tacaaatata aaagttcttg aagaagcatt aagatacctt ttagacacta
      421 acataagatt aattaattta agtttatcag tcataggagt agaaagtgtt aagggcttat
      481 ttgaagtttg ttatgaattg tttagaaaag gaaaaataat agtatgttca ctcgctaatg
      541 attttgactt aagttatcca gctatgttta acaatgttat aggtgtaaga ggatttactt
      601 tagatggaga tgattcattt tggtacaaca aaaaatatga tgttcaatgt gttatggata
      661 gcaattcata tataagttgt gatataaata attcctatag attaccacca aaatgtaata
      721 gttatttatc agcaaagctt actggtaaaa tagctaagat attatcagaa aaaccaggca
      781 taacaatatc tgatttaaat gataagcttg aatcattagc aactagaaat cattgggata
      841 gttgtgattt tgatgagtgt agtagaattg taaactttaa attggatttg tatgacaaag
      901 aaaatgctct ttttatgaaa gttgctgacg ttgttaggga ttgcttagat attgaagaga
      961 ataatgaaaa actctttaag ggtagccttt ttaataagga gataggcttg gtttatgata
     1021 attgttttaa tctgttgaaa aagctagagc atagatttga cataaagttt aattatatgg
     1081 acatctcaaa atatgactta gtttcaatat atacactgac tgaattagtg gaaagatata
     1141 aaaacttata aataaagatt tgtagctaaa tttaatgtag aaggtattaa attatatgta
     1201 ttctttgtcg gtagattaaa agtaaaaaat atactataat tagatagcat aagagaaaat
     1261 gccaatacag ggggagttag ataatgaaat atgaagaagc attagagtac atatcacaaa
     1321 ctaataaatt cggtataaga ttaggattag aaaatatcgg aaagttatta gaattattag
     1381 gaaatccaca agaaactttg aatataatac atgtagctgg aactaatgga aaaggttctg
     1441 tgtgttcttt tgtttctaat atactaagag agtgtggata taaagttggt ttatatacat
     1501 ctccatattt agaaactttt actgagcgta taagagtaaa tggacaaaat ataccacagg
     1561 atgatgttgc tagaataatt gagttaataa aagaaaaaat agagattatg gtaaaagaag
     1621 gctatgcata tccaacagaa tttgaagtgg ttactgcaat ggcattttat tactattcag
     1681 aacaaaaggt agactttgtg gcattagaag ttggtttagg tggcagatat gatgctacaa
     1741 atataataac aaaatcactt gtaagtgtga tagtatctat aagtttggac catacaggta
     1801 tacttggaga tacaatagaa aaaatagcgt acgaaaaggc tggaataata aaggaaaatg
     1861 gagtagtact agtatatgac cagactgatg aagcaaagga tgttataaag tcagtttgta
     1921 aagagaaaaa agctaaatat atagaagttg attttgatga tataaacata aaaaaatcag
     1981 atataaattc tcaaatatac gattgtactg taatgaaaga aacttataga aaccttgaaa
     2041 taaaattgat aggcgaacat caaataaaca attctatctt ggcaattagt gtaataaaat
     2101 atctaaaaga cataaataaa ttagcaaata taaatgaaga atctataaga aaaggactta
     2161 taaatactaa atggccagga agaatagaaa agataaaaga aaatccaatt tttataatag
     2221 atggtgcaca taatgaagat ggagcaaaat ctttggcaaa agcacttgat aagaatttta
     2281 aaggtagaaa attaacctta ttaataggta tgttagaaga taaagatatt gatagtgttt
     2341 tagagatatt gttgccacat tttaataagg taataacaac aacaccaaat aacccaaggg
     2401 ccataaattc agatatatta agagaaaaag tattaaaata tgtagatgat gtaacatcaa
     2461 agcatgagat agaggatgca gtaaattata cattagaaac atcaagtgag gatgatatta
     2521 taataagtgc aggttcatta tatatgattg gtacagtgag aactttagta aagaaattat
     2581 aattttataa aataagtata caaataaaaa tataaaaaag agttgtctaa aaaatggaca
     2641 actcttttta tataaaataa gtttttaata attttactga tgtttatata agtaattata
     2701 tagattgacg tatagcttta gataattggt ctgggcaaga agtacctttt ccaccacagt
     2761 caataccatt taatttatca gcaacttcaa gagcattttg gccaattatt aagcttctaa
     2821 gtcctgataa attaccatta catcctccta taaactcagc atcaacaatc acattatttt
     2881 catcaacttc aaaaatcatt tctctacaac atacaccact tggattaaat gttactttca
     2941 ttaaaaagac ctccattaaa tattatttct ttttttaaca ttaataataa taccaaatat
     3001 atgcgtaata atgcaactat aaagcataat acaagctaat gaaataatat attattgtag
     3061 tatatgtatt gtagagagga ggggttatat gaaaatctat atttctaact acttatcaaa
     3121 gagtattagc atagttgatt attctacact tgaactagaa aaagaaatag tattagaaga
     3181 taatatatat ccacaccatt tttgcataga aaaagaaaaa aatttaatat acatacctag
     3241 ttcaagtaat gggatactct atgtcttaga tttaagtaat gataaaataa ttgatactgt
     3301 ctctatagga ggaagtctta gtcaagttgt acttagcgat aatgaattgt ttgtggcaaa
     3361 tgaagattct aatagtatat atgtactaga taaaaacaca ttaaatccaa taggaattat
     3421 tggagttgat aatatgccac atggatttga ctttgataga gaaagcaaaa agttatatgt
     3481 accgtgtata aactcgatag tatgtataga tacaattaat aaaaatatac aaaagaaaat
     3541 aaatatggac tttagagctt ggcatataga acttgataaa caaaagaaag aaatgtatat
     3601 atcaacactt gatggaaagc ttataataat agatgaagta agcatggata taaaaaaggt
     3661 attatatgaa tttttattac cagtagaaat aagatttaat tatagtggca aaaaagttta
     3721 tgttgctgat ttgggttata ataatgtaag aattttagat tatacaacga gtagatacat
     3781 tggaaatatt gaaataaatg ggattcccca aggattagaa atatctaaag atgaaaagtt
     3841 actatttgta tcagatacac aagaaaattc agtaaaagtt tatgaaactg caaataacaa
     3901 attaataaaa gaaatcaagg taggaaaaga gcctacaact ataatttgtt tgtaggcttt
     3961 aatgtatgtc tcttataagt aaatcgataa cttcggcata catataagat gcattatttt
     4021 tccagttatt gcaaataagt tttgcttgtt tctctgatga aacatttaga tttaaatcta
     4081 ttaagttaga ctgattttct atgactctaa ggttgacaat aaattcatta tcactttgtt
     4141 ttacaaaact agactttact tgagtatctg ctagtagatt ttctttgtta gaatctatat
     4201 acttgtctat tttttcagta acactagtag gtattcgatt aaagaagtat tctagacttt
     4261 ctacgcctct ttgagtaaga gcataatatt ctctgtcaga gtctttatat gtagtcaaaa
     4321 acttagattc cataagctga gataaaagct gttgtaatga aaaataattc atcatctcag
     4381 tctctaatac tacttgagtt atttgagaat tagttaaatc cattttaatt ttttctaata
     4441 tatataaaat taaaagtttg tgataggcta attcttcaga cgagttttca aacataaata
     4501 tcatcctccg tcaaactatt tctatatata atatattaaa ctaatttgta caaaattaaa
     4561 agaacaattt gtattgtatt attaatcttt aataattcat ataaaaacaa ttttataaat
     4621 aaatatagaa aaacagggct gctaattagc agccctgttg atacccaggt gtcaaaattt
     4681 actatttatt ttccagccat ttgtctttca gccatttcaa ctaatttctt agtcatatat
     4741 ccaccaacat atccattttc tctagctgtt aagttacctt tgtctatgtt ttcatagtta
     4801 gataaaccta tttcgttagc tatttctaat ttcatttggt ttaaagcagc ttttgcttct
     4861 ggaactactg ttctgtttga atttgacata gtaaatttcc tcctcaaata attagtttat
     4921 gatgtctaac aacactttta tttatgtgat gttaatacta atttctccaa aataaaaata
     4981 atatatacag aaaaataaag gtaaaaatgt aaaaaatata aaaaaattaa ataaatgagt
     5041 attttagaga gtttaattag gtcaatatag gttttatttt tttagttatt gtcttataaa
     5101 ttaaatatta cagtactttt ttgagctgaa aaaaaataaa ttttaaatat agaagaaatt
     5161 atttcaaagg gggaatagta tgaaaagaga agttaaaagg actaaagctt tgatatatag
     5221 tgttttattt ttttcattat tattagtgtt aatatttgtt tttgtttatg gaataaaata
     5281 catatgttct aaatctgaca attcaaaaat gcctatatat aaagttgata caaatgagaa
     5341 aaaaatagct ttgagttttg atgtagcatg gggaactgat aatatggatg atattttaaa
     5401 aattttagat aaacataatg taaaagctac cttcttttta gttggtagtt gggtggatga
     5461 taatgaagaa attgtaaaag ctatagatga aaaaggacat gaaattggaa atcattcaaa
     5521 tactcatgcc aatttgaagg aaatatcaaa agaagacata acaaaagaga tagaaactac
     5581 atcagaaaaa atatataata taacaggaaa aaggactaat ttatttagac ctccttttgg
     5641 ggacgtaaac aataaagcta tggacatttg tagtgattta ggttataaag taattaagtg
     5701 ggatgtagat tctatagatt ggaaggagct tggaccaaat catgtaattg agaaagtaat
     5761 aaaagaatct caacctggtt caatagtttt attccatgct aatataaatg atgtagataa
     5821 ctatttggat actataataa gtcgactaaa aaaagatgga tacagtctag tcaaaatatc
     5881 tgatttgttg tataaagata attatgtagt tgattccaat ggagtgcaaa aaactaagaa
     5941 ttaataagta ataaatgtat attttaagta aagttgtgat aaattatata gtatatgtta
     6001 gaaaagtgtc gataaaaaga cggtgtctat tttttgacac tttttataca tgtatgcaat
     6061 atcaataatt atagattaaa ttgattaaaa aagtgtctaa taattgactt ttttttgatg
     6121 ggtttaaaaa tttagaagaa ttaatgaatg ctgtaagaca gtatttccaa agggtataag
     6181 tttgtttaaa agttggcata gaaattgctt tgttaataag cgatatgttc tatagaaata
     6241 aacaatatgt taagacctta taatagaaaa atagcattaa cattatttga acagcttata
     6301 aaaaaattaa tatacttaag ggggcgtact agttgaacaa actatattct attttattaa
     6361 aacaacacgt tggtggtcca gataagccag tagtaagtgt tggagatgta gtaaaaaaag
     6421 gaactctaat cgcagaacca actggattag gggcaaatat ttatgccagt gttagtggaa
     6481 aaataagtga aataaatgac caagcgatag taattgaagc tgatgaagta caagaagata
     6541 cttttgaacc attaaaagga gaaggtatac ttgacttaat taaagaagct ggagtagtag
     6601 gaatgggtgg agcaggattc cctactcatg taaaattaaa catagattta aatggtggaa
     6661 caatattagc aaatgcagct gaatgtgaac ctttattagc tcataatata aaagaaatag
     6721 aagaaagacc agaaatagtt tatcaaggta taaaatatgc tatggaagtt actaatgctg
     6781 gaaaaggtat gctagcaata aaaagtaagc atccaaaagc aatagaagct ttcaaaaaag
     6841 taataaagcc aggagacaac atagaagttg cagaattagt tgatatgtac ccaatgggag
     6901 aagagagagc aatagtaaga gacgttctag gaaaattact tgagccaact caattacctt
     6961 cagaggctaa tgcagtagta ataaacgtag aaacactaac tagaatagta gaggctgttg
     7021 agcaaaaaag accagttata tctaaaaata taactgtagt tggtcaatta aacagtggaa
     7081 aagaatctat agtgtttgaa gatgtgccaa ttggaacaac tgtcggagaa ttaattgaaa
     7141 gagctggtgg aataaaaggt gaatatggtg aaataatcct aggaggacct ttcactggta
     7201 aagctactac tttagatgct ccaataacta aaactagtgg tggtataata gttactatgc
     7261 catttgttaa tgaaaagaga aaaatgggtc ttttagtttg tgcatgtgga cctaatgaag
     7321 aaagaatgag agatatagca actaaaatgg gagttactga tatagtatca gttcaaaaat
     7381 gtaaacaagc tcaagaaatt aaaggtgcat taaaatgtga aaatccagga cactgtccag
     7441 gacaagctca aaaatgtatt gaatttaaga aagctggagc agaagttata ttaataggaa
     7501 actgtactga ctgtagtaac acagtaatgg gttctgcacc aaaattaaaa ttaggtgtat
     7561 atcatattac tgaccatgta atgagaacag ttaatcatcc attaataaga agaataaaag
     7621 gtgacgtaag attaaaaata aaataataga taaatagaag ttagacatct ttagaagtaa
     7681 aacttaaaag ttacttctaa agatgttttt taatgtattt tgagggcaat atatattgta
     7741 aaataaatgg aaaaaataga caaaatatgc tagatattgt ataatgataa caatagctat
     7801 tacaattaat attggacaaa aataatgata ctcaagtatg tggtttgtat taatatgcat
     7861 attatattaa gtcaactatc cgatagagta aaaggttttt gtgatatttg aagatactct
     7921 atagtatctt ttttattata aaaactaatt tttgtctttt attagataag tatctaggat
     7981 actaatttat attttattga gttgttaaaa aagtgcactt aaaaagttaa aaaagaaaaa
     8041 ttaaaaaacc tatttataga aactgtgaag tatatcttaa acctgggcac ttaaaagata
     8101 tatggagtta gtagtgcaac ctgctataaa tataaatagg agtaactttt aattgtcaaa
     8161 aatatataaa ataaatacat aaataagtaa ttaatagaag atagatataa atatattttt
     8221 gaaatgctcc ctgcattcat aacgagtgta gagtggcaga tatctgccca aaactccttt
     8281 aattgctgga agttcctaaa gctaactaaa ccacaacata atcatgaaat aaggataagt
     8341 gtgatagagt gtgtatttaa taagcacttg aggttacgaa agtagaaaaa attagttaga
     8401 tagcataagg ttaaatccta agtgccttga aaatggataa tcagcaggta agcttcgaaa
     8461 agaagaaact tcaacgacta gagagaaatc tcgtacctac aagtgtgggg aaatggggag
     8521 tacctaattt atggttaggt aatgagatag tctgtgctta atagaaatat taagaagttc
     8581 ataggagaac tggttaggag taacgaccct aattgaacac actaaagatg tattattgaa
     8641 taagattcat ataatattta ataattttaa ttatattaag tgaatacaat atatgttttt
     8701 agtatcagga gaattttgaa aacaaaaatt aaaaaatcaa gtataatcag tttagtaatt
     8761 gcttttagta tggtatttac tgcatttaca ccaatagtat cttatgctga cgaagtgact
     8821 agcaatgata caatacttaa tggagaagaa caatcaaatg gtcagacacc agatgtagaa
     8881 aaaccatcag acggtcaggt accagatggg gagaaaccat cagacagtca agtaccagat
     8941 ggggagaaac catcagatgg tcagatacca gatggagaga aactatcaga tggtcagata
     9001 ccagatggag agaaaccatc agatggtcag ataccagatg gggagaaacc attagatgag
     9061 caaactccag aagaagaaaa gccattggaa gaagaaatag ttatagagga aatgagttta
     9121 aaacaagata ttgataaaat tcttgatatg actttatctc aaataaataa aatagtatat
     9181 aacttttggg aagatgaaga agatgtaaag gtagatgaac aatcagaaat taaccaagtt
     9241 tttacttcag aagactcatt tattagctta tggtatgaca aaaaagcaaa ggtgaaaaat
     9301 tcttgtttac taaaagaaat agatggttca gatagaccct atcatgattt aagatttgat
     9361 gatattacaa agactttaac ttttgactat gtaataaagg gtcttgtagg agcaagctca
     9421 aaagacgaca aatatgtaat agaaggtgaa gatggagagc aaactgcatt ttgttataat
     9481 aatcatttaa gaccaccaag tagtaatgga aagagtccat atttaccagc agaagatttt
     9541 aatggtcaag aaaaccaaaa tgaagaagca gttaaatcta tactatatgc aggttctgaa
     9601 tttgatggat ttggatataa gcaacaattt aatttaggcg aagaagaaaa tgacataatg
     9661 acttactcag ctactcaaag tgcaatttgg attattcttg gacaaataga tgaacaagaa
     9721 agacttaagc agtatcaagg ctctatagac ctttgtgata aacttataga aatagcaaaa
     9781 actgaagagg aaagagctga gtatcagaag aggaaagaag tagcgataaa tacaaaagct
     9841 tatttagaag cattgttaaa agcaggtcgt gaagagttaa agccaaatga tacaggaaaa
     9901 cctagtttga gtaatggttt aactaagatt aactttgaaa aaaatgaaga tggtacttat
     9961 gaaacagaag ctgtagcctt agtaggttat agtggtgtag taaagttaaa actaccagat
    10021 ggagtaactg cttatgatgt agatggtaat ataataggta caggcgaagt agaaatttca
    10081 acgcaacaga aatttaaact taagagtgtt ggaaaaccag atgccaaagc taatatttct
    10141 gctgtatctt atgattatat ttttccaaaa gctattcaat attataaggc agtgcttgat
    10201 ttaggacaaa aagaccattc atctttatta ccatctagca agcaaaattt attaagttat
    10261 acaatagaga aaaagaatgg tcaagaagtt aactttaata taggtttacc aacagatgat
    10321 gatgatgttg taaatccacc agtgccacca atagatgatg atgttgtaaa tccaccggtg
    10381 ccaccaacag atgatacaaa tggacataag ccaaaaccat caccaccaat agatgataca
    10441 gtaataaatc caccagtacc accaatggat gatacaataa taaatccacc agtaccacca
    10501 acggaggatg cagtgttaaa tccaccagtg ccaccaatgg aggatgcagt gctaaatcca
    10561 ccagtgccac caacagatga tacagtgtta aatccaccag taccaccagt aagtgataca
    10621 gtggaaaaaa ctccagaact atcaagagat gatacaatag taaaatcacc aaaaacaggt
    10681 gatgaaactc agctaatgtc atatgtattt attagtgtca ttgcaatttg tggattagcg
    10741 tatcaatgta aaataaaaag aaattaatct agattattat taagtttaaa ataaaaagtc
    10801 tcaaattgat ttataagtca ttttggggct tttttatgta aatataatat gcaaatatag
    10861 tttgtttaaa aatatctatt ttaattatat tcttaaattt atgattggat acaggcattc
    10921 attaattgtt tgatgaatag aaaaatgttt tgaaggtga
//
LOCUS       DACXMZ010000129        10912 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.130, whole genome shotgun sequence.
ACCESSION   DACXMZ010000129 DACXMZ010000000
VERSION     DACXMZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.130"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            6..590
                     /locus_tag="JD016_12690"
     CDS             6..590
                     /locus_tag="JD016_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HAU5000493.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(684..1463)
                     /locus_tag="JD016_12695"
     CDS             complement(684..1463)
                     /locus_tag="JD016_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAU5000494.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(1521..2777)
                     /locus_tag="JD016_12700"
     CDS             complement(1521..2777)
                     /locus_tag="JD016_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAU5000495.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            3035..3934
                     /locus_tag="JD016_12705"
     CDS             3035..3934
                     /locus_tag="JD016_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAU5000496.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            3958..5406
                     /locus_tag="JD016_12710"
     CDS             3958..5406
                     /locus_tag="JD016_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAU5000497.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ATELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            5465..6271
                     /locus_tag="JD016_12715"
     CDS             5465..6271
                     /locus_tag="JD016_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000498.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            6297..8216
                     /locus_tag="JD016_12720"
     CDS             6297..8216
                     /locus_tag="JD016_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAU5000499.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(8458..8805)
                     /locus_tag="JD016_12725"
     CDS             complement(8458..8805)
                     /locus_tag="JD016_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HAU5000500.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(8792..10720)
                     /locus_tag="JD016_12730"
     CDS             complement(8792..10720)
                     /locus_tag="JD016_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HAU5000501.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKIPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
BASE COUNT         4063 a         1631 c         1494 g         3724 t
ORIGIN      
        1 taaatatgtt aatgaatacc cattttatta tggcaaaatc aatacttgat aatatagatg
       61 aaaataaaac attctttata agcgaaaaaa attttatata tggcaatata aagcctgatg
      121 ctttttctaa atataaatta aaaaaacatt atttagatga gtcatttaat atgattgttt
      181 ctaaaataaa ctatctatgc agtcttaata tagatagtct ttctaaatac ttttcagtaa
      241 gtagacttag tcaggaacta ggagtcatat gtcatttttt atgtgatttt ttctgtgtag
      301 ctcacagtga acgttgggaa tttaagcata gcttaaataa acatgttact tatgaaagag
      361 aacttggagc atttgcaaaa gatgttgatt tatctaaaat taaatgtggt actataaata
      421 gtagttttga gaagtttttt actaaacttt atagtgatta caaaaagaaa actgattata
      481 tgaatgattt aaaattttca gcttatactt gtaatagtgt cataaattat atacttgact
      541 ctattttatc taatacaata aaatcatatc atatagcaaa ttgcggttaa cttaagaatt
      601 tttaaactta taattgaata taattaaaat gactgctcta tagaatataa ttttatactt
      661 ctctaggaca gtcatttttt atactaacta tttatctcca ttataacatc tatggcactt
      721 tttacactat ctactacata atttgcactt tgctttacat cttcccttgc cttagacatt
      781 gcaaaactat atttaaatcc ttctaacatg caaatatcat ttggagaatc cccaatagta
      841 gcaacttcat ctggacttac atttaacgtt tttattacat gctctatact tcctcttttt
      901 gacacacttt taggaactat atttaaagta tatgttccag aaaaatatat ttctaagact
      961 tcactaaact cactttttag ataaagttct attctggata actcttccaa attttcagaa
     1021 catacgccta tatttcctat aagattatca aaactacttc ttttttcaaa atctgggtca
     1081 actataaagt cactagaata atgtctaaaa gcagtggtat cactacccac aattacatca
     1141 cccttatatt taaaataaag ttgtgcatca cttgactcaa atctatcaat gacttttttt
     1201 actaaattat catctatcgt tcttttaaaa attacattat aatctttatc atatataaat
     1261 gaaccatttt cacatacata gtatccattc atatctaaac tatctttgat aatattatgt
     1321 gcttgtttaa aaattctacc tgtagcaaca ttaaactgta ctcccttttt ttctatttct
     1381 tcaattgcta ctatatcttc ttttgttatg gttccatttt catatagagt tccatctaaa
     1441 tcacaaaata tatgttttat cataaattaa aagcctccaa aaacaatttt atattttact
     1501 aatactttaa tcaaaataac ttaaagcttt tctaattgta ttctcaattc attaagcaaa
     1561 tctctagtag ctcttaatct tatctttctt ctatccccat taaatatatt tttctttact
     1621 attgtttttc catttatata tattgcagta taaaccagtc ccacaggctt ctctttggtt
     1681 cctccaccag gtccagctac tccagttgta gatataccta tattagcttt aagtccttta
     1741 gcaacgccct cagccatttc tatagctgtt tgctcactta ctgctccaaa ctcttccaaa
     1801 gtttctcttt ttactcctaa tcttttcatt ttagaatcat tagaatatgt tacacaacct
     1861 tcaagaaata cagaggatat acctggatag tttataagag atgaagaaac caaacctcca
     1921 gtacaagatt cagccactgc tattgttaga tttttttcta caagtagctt agaaacagca
     1981 tcttctaaag cagtctcccc ttcagatatg gaaatatctc cttcagtata tacatattta
     2041 ccaactctac tttttatttt ttcttctact ggttttatta atttaaaagc ttctctttca
     2101 ttttcagcct ttgcagttat tcttattgta acttctaatt cttttgcata taaagcaact
     2161 gttggattag tctgctcatt aattatatcg agaatttcta attctagatt tgactcacct
     2221 attccatata atcttaaagt cttagatact agcatttcat ttgtgaattg ttgtaagtaa
     2281 ggtttgacac tctcattaaa catagctttc atttctcttg gaggtccagg caatactatt
     2341 ataaatttac catctttttt taaaattgcc cctggagctg taccattatt attttttagt
     2401 actatagcat cttttggaaa gtacacttgt ttcatattat tttcggccat ttttttccct
     2461 gttttaacaa agaaagattc caaaacctct agtgaaggtt tatgtagttc taatttctga
     2521 ccaaagtatt ctgcagcagt ttcttttgtc atatcatcac cagttggccc aagaccacct
     2581 gtagtaatta caaaatcact tctctttaag ctttcatcaa aacattctaa aagtctttgt
     2641 ttgttatctc caacagtact ctgatggtat acttctattc ccaatgatgc aagttcttta
     2701 gctaaaaatt gagaatttgt atttactata tctcccaata atatttctgt tcctacactt
     2761 attatttcag ctttcaaaat ttatcactcc ttgatttata gccattaaga ctagtcttaa
     2821 gttcataaca ataattttag caaaaataat aacataaata aagttaaaaa atcatttaaa
     2881 catatcttta aactagatta ttattctaaa accacacata atataaactt cgtatatagt
     2941 ggtactttca tcctcaactg ttaattcata taattaagta tgtaaataaa aagtttactt
     3001 taaaataata aaaattaaaa taggagtgta taatatgaaa atagcagtta taatgggtgg
     3061 aatatcatca gagagagaag tttctttaaa ttcaggaaaa gagatatata ataaccttga
     3121 taaaaataaa tatgaagttg taaaggtaat aatagatgat aaaaaagata tatttacaaa
     3181 gataccagaa gatatagatt ttgcaatact tgctttacat ggaaaatttg gtgaagatgg
     3241 atgtatacaa tcaatacttg agactatgga tataccatac tctggatgtg gacctctttg
     3301 cagtgggatg tgtatggata aaaatataac taaaaaaatg cttagagatt caaacctacc
     3361 tactgctcca tgggttttag taaaatcagt tgatgaaata gactatgatg aaatagataa
     3421 tataggttat ccagtattta taaaaccaaa ctcaggaggt tcaagtgtag ccacattctt
     3481 tattcattct aaggatgaag ttgaagaagc agttagaaaa ggtcttgaag ttgatgaatt
     3541 tgtaatgatt gaaaaatata taccaggtgg agaatatact tcattcatat taaatggaga
     3601 agtgttccct acaatatcta taaaatctga ttcaggattc tttgattatg aagctaaata
     3661 ctcagttgaa aaaggtgcaa aggaagaagt tgtttactta gatgaagaac ttcaaaaaag
     3721 agtaaacgaa atttctgaga cttgttggaa aatttttaat tgcaaagcat atgtaagagt
     3781 tgatatgatt ataagtgaag gtattccata tgttcttgaa ctaaatactc ttcctggaat
     3841 gactcaaact agtttaattc ctagaagtgc tgctgctaga ggtattaagt acagtgaact
     3901 attagataaa cttatagaat actctttaaa ttagatgaaa taatttgagg tggtaatttg
     3961 atattttcaa atctaaaact aaatgataat gaaccaattt atatacaact taaaaattat
     4021 ataagtgata tgatatcaaa aggactaatt ccagacaata gtaagctccc ttctacaagg
     4081 gagcttagtc agcttttaca agttagccga aattctgtag ttttaactta tgaagaatta
     4141 aaatcagaag gattaatcta ctctatttca ggaaaaggta catttgttaa atcaaagaat
     4201 aagtcttcaa atactacatg gtcattaaac tgggattgtt tagagaatac atactctaaa
     4261 aaagccactg aattggacat tataaaaagt gaaattcctt ggagctctga cctgatttcc
     4321 tttaaaagta tttctccaga tggagattta ttcgacatgg aagaattaaa aaaatccttt
     4381 ttaaatagaa tttcactaga aggacataaa ttactcaact atggatatgc acaaggttat
     4441 aagccactta ttgactattt actagaatat atgacaaaca agggtgctga tataagtaat
     4501 aaagatatac taataacaaa tggattcaca gaaggctttg atattattat gtcatctttt
     4561 acccaagaaa aagactacat aatatgtgaa aatcctactc ataatactgc tataaaaatt
     4621 atgaaatctc ataacctcaa tatcttaggt gtagatataa atgaagatgg tttggacttc
     4681 aatatgctag aagacaattt agttaagtat aaaaataaaa taaagtttgc atacattaca
     4741 ccctcttatc acaatcctac aggaattgta atgactcctg aaaataggta caaattctat
     4801 aatcttatga aaaaatacaa tatagcaatt atagaagatg gatttaatga agaattactc
     4861 tacaatagtt ctcatatatt tcctatctgt tccctagata atatgaataa tggggtcgta
     4921 tatattggta gtttttcaaa aattttattt cctggaatga gaattggatg gatatttgca
     4981 gataaaaaag taatcaataa gctagaaagt gtaaaaaggt gcagaaacat acatgtatcc
     5041 tttttagacc aaggaatttt atttgattat ttaagtaatg gaggttttga aaaatacatt
     5101 aaaaaaatac gtaaattcta tggagacaaa tttaactttg catataagtg tataaaaaaa
     5161 tatataaaaa cagaatatat cttgggtgat ggtggtttgc atatatttat aaaactaaaa
     5221 aatatagata ctagagtttt attagaaaaa tgttatgaaa aaaatgttat atttatgcct
     5281 ggagatttat tttttacaga taactctggt tttgatactt taagactcgg attttctagg
     5341 ttgtcttttg aagacattga aattggaata aaaataattg gagaaactat aaatgaaatg
     5401 ttctaggtta actaaatatt gttagcctag tttattttta tgaggtataa tatatatagt
     5461 ttatatgaat ctaatactgg gggataatat gagtaaaaag tcaaaacgaa gaaaaattaa
     5521 taaattaaaa ttaacgattg taataactgt attatttctt gctttattat ctatatatga
     5581 gatactgttc tctaatccca acaaaaaagg taatataaag agtcaagaaa ctatgcaaaa
     5641 taaaaataat acaacagatg attctaaaaa taatcaaagc tctaccaaaa agcaggaaga
     5701 taataatatc aaacctactt tttcatattc ttcaatacca gataatataa aaaataaaat
     5761 gattggtaaa tctatgccta ctgatgaacc tataagcttt gatagtctat cttatttaaa
     5821 acttacatat tatggatttg atgaaaaaac tcatcaagga gaaatgatag taaacagtga
     5881 actagctcca gaagtagtgg atatttttaa ggaattatac gaaaaaaaat atccaattga
     5941 aaaaattaag ttaatagatg actatgatgc tgtagatgaa aaatctatgt ctgataataa
     6001 tacctcatca ttttgttata gaactatagc tggtacaaat gtagtttcaa atcatggtaa
     6061 aggacgtgct atagatataa acccattaca gaatcctcaa gtttctggaa atgatgttac
     6121 acctaaagta agcacagttt atgctgatag gtctagcaca aaatttggaa tgataaaaaa
     6181 aggtgatgat tgttataatg cttttgtaag cagaggttgg agttggggtg gctattggaa
     6241 aaacccagat tatcaacatt ttgaaaaata atagattaga aataaaggtg aattatatga
     6301 atttgatgac attggaaaat ataagtaaaa gctattctga aaaaaaatta ctagaaaata
     6361 tatctcttgg aataaatgaa ggggaaaaaa taggacttat tggtgtaaat ggtactggta
     6421 aatctacctt actcaaaatt attgcaggag ttgaagaaag tgagactggt aatataacaa
     6481 aaactaatgg tattaggata gaatatctac ctcaaaatcc aacttacaat gaaaattcaa
     6541 ctgtacttga acaagtattt aatggaactt ctgaagaatt aaagctactt ggtgaatatc
     6601 aagaaatcct agaaaaactt aattcaaact ttacagagga attaaataaa aaattattat
     6661 cagtacacga aaaaatagat gccttaaatc tttgggattt agaaagtgaa gctaagtcag
     6721 tacttacaaa gcttggcata aatgacttta atcaaaaaat agcagaactt tctggtgggc
     6781 aaagaaagag agtctctctg gcatcagccc ttattacacc ttgtgagttt ttgatactag
     6841 atgagcctac aaaccatcta gacaatgata ctatagattg gcttgaggaa tatcttaatt
     6901 ccagaaaagg ttcattgctt atgataactc atgatagata ctttttagac cgtgttacaa
     6961 atagaattat tgaactggac aagggtagat tattcagtta tgatgggaat tattcaatat
     7021 ttttggaaaa gaaaatggaa agactttcgc tagaagcaag tattgaagat aaaagacaaa
     7081 atttaataag aacagaactt gcgtgggtaa gacgaggagc tcaggctcgt agcactaaac
     7141 aaaaagctcg tttacaaaga tttgatgaac ttataaatag agattctttt aaacctgatg
     7201 aaaatcttga tatatctgtt gcatcaagta gacttggaaa taaaataata gaaatacaca
     7261 atatatctaa atcatttgaa gaaaatactg ttatagataa tttagaatac actttaactc
     7321 gtacagatag aattggtata ataggcaaaa atggtatggg taaatctacc cttataaata
     7381 tacttaatgg agaaatagag cctgatagtg gatatatttc aattggagaa acggttaaaa
     7441 taggttgttt ttctcaagac gactctcata tgaatattga tatgagggct atagactatg
     7501 taaaagaagc tagtgactat atagaaactt cagatggtac taggattaca gcttctcaaa
     7561 tgtgtgaaag attccttttt aatggtacta tgcaatatac tttgataggt aaattatctg
     7621 gtggtgaaag aagaagacta catcttctta aaatccttat gacagctcct aatgtgcttc
     7681 ttttagatga acctacaaat gatttagata ttgaaacgct taaaatatta gaagaatacc
     7741 tagacgaatt taaaggtgta gtagttacag tatcacatga tagatatttc ttagatagaa
     7801 tttgtaataa aatatttgca tatgaaggta atggaaaaat tcatatctat acaggtaatt
     7861 acagtgatta tcttatttat agagagattc aaggaattga atttgaagaa tctaaaaagg
     7921 aatcagttaa aaaagatgac tctactaccc taaaaaaaga aaaacctaaa aatgataaaa
     7981 agcttaagtt ctcatataat gaacaaagag agtttgaaaa tattgactct gatatagaaa
     8041 aactcgaatc taaaattgca acacttgatg aaagtactgc aaaattttca actgatttta
     8101 ctaaacttca agaaatttta gatgagaaat ctaaactaga aaaagaactt gaatacaaat
     8161 atgaaagatg ggaatattta aataatctag ctgatgaaat agctaataat aagtgatatt
     8221 aaaataatct taagtttaat aagttttact ttaaatctgt actttataaa cattttaaag
     8281 gaagtgtctc aaaatgaact ttttagtttt atgagacact cttttttatg agaaatcaaa
     8341 tatgttttca tcatttcaac aatacaaaag aggcttatct ctattttgag acagcctctt
     8401 tcataattac cttctaattt atatgctcta atatatctat aataattctt attatatcta
     8461 tatatccagt ctttcaatat agtgacaatt aacttcaata tgatttaaaa atactaacaa
     8521 cctttcataa agtggatgag attcttctcc atatttagct tctaggttct tgcctatttc
     8581 tctaacagtc ctttgaccat caatttgtaa aaaaacaaaa ctggaatatt catctagttc
     8641 tatttttttg tatttaggta ttctagcttt taactttcta aaaaactttt gaattttatg
     8701 gtcctgtttt tcaagtatag taacaacact attttcagct acttcatatt caagtccatc
     8761 agtaatttta aatacaatat ctaaaacatc attactcttg ctcattattt tttactttag
     8821 ataaaacaat aggtaatgct gtaagcacaa ctaaaactac caataaagca attgccattg
     8881 aatttgttgc agcaaatcca cttggtatct ttggagttac aactccagtt acttgtaata
     8941 ttatacctat taaaccaatg atagaaccac cagcaacaag accagaagat aaacttatac
     9001 cattagaaac tttaacttct ttttcttttt cagttttaga tactaattca acaaataaac
     9061 gaattaatgc tccaactaat attatagaag ttgttgcaat tggtaaatag aatcctatag
     9121 ctatagtcat tattggtaaa tttaatgcat atagaactat tgccataaat actccaacaa
     9181 taatcataac ccaaggtaaa ctacctgaca taataccaga agttaatgtt gacattaagt
     9241 ttgcttgtgg taatgcaaat tgtacattat caccagttgc tctaagttgg tcagaaagta
     9301 ataaaattac tcctacaaca actataacac caactatact tgcaattgta aagtaacgtt
     9361 gcatttcgtt tttgctacca ccaataatat aagttacttt ttgagattga gtataacctc
     9421 cagctatagc aatagcaaca actataaatg aaccaaataa aagtaatgat ttattagctt
     9481 ctaaatcagt ccatcccatt ataacaaaca ctaatgttac aataactaga gaagctatag
     9541 tcataccaga aactggaaga tttgatgttc cgattgttcc tgttaaacga ccagctacta
     9601 tgacaaataa taaagataat aaaagagata tgatagctcc aataattgcc ataacaatgt
     9661 taccagaaat taagaatgct gctaaaaatc caataactac tccacctaat aaaagtatca
     9721 tttgtataga tgaaccttct tctccctctc cagcacttga tttagcttta agtgtttctt
     9781 ttatagaagc gattatagtt ggtataagtt ttattgcacc aataatacct ccacaaagca
     9841 tcataccagc acctatatat tttacataac tactagatat tgctccaaca tccatttggt
     9901 taagaggcat tgcaggatta ttccatacca tagctccatc tttagccata tctgtaaagt
     9961 atcctattaa aggtgctata ccaaagtttg ataaaatact tcctgcaaac attgttaaag
    10021 aaacttcaag ccctactata aatccaattc ctaaaagtag aggatttact tcaatatcaa
    10081 atttccattt gtagaatttg cttcctacaa gactcattac attgtttgct acatttaaga
    10141 atgaaccagt taatacagta ataaatccac ttattacaaa tccaattccc atgtacttaa
    10201 ttgaatctcc accagcctct gaagcaacaa gtgtttcaga tatagccatt gattctgggt
    10261 acataagttt accatgttct tcaactatta gatagttgtg aactatagca gcaattccta
    10321 taccaaataa aactccacca actccaacag ctaagccttc aaagaatgta atttggcttc
    10381 caattaatat aactgctggt aatacgaaaa taaatccact tgcaacagat tctccaccac
    10441 ttgacatacc ttggattaag tttttaccaa gaataccctt tgaacgagca aatgctccaa
    10501 caaaagcaga acctattata gctcctggaa tacctgcggc aactgtaaga ccggctttca
    10561 ttccagagta agttgtagat gcggcaaaaa tggcagctaa aataataccg ataatcagta
    10621 cagctacatt tccaccggtt ctggacttat ctgtgatata tggaacgtaa tcttttccac
    10681 taacctcacc ataagcaccc ttaggtaatt ttttgttcat aaaattttca ccccttttca
    10741 atttgtatat attataacat atatacatat aaaacttaac tttttattat tttacaaata
    10801 gcgagtcaaa actactaaaa tttaacccta tattattaat ttctactttt agatattttt
    10861 atgctaattt tctttttatt tttcaattct actaaaaact attaagatat aa
//
LOCUS       DACXMZ010000130        10852 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.131, whole genome shotgun sequence.
ACCESSION   DACXMZ010000130 DACXMZ010000000
VERSION     DACXMZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.131"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            92..796
                     /locus_tag="JD016_12735"
     CDS             92..796
                     /locus_tag="JD016_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HAU5000502.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            793..1563
                     /locus_tag="JD016_12740"
     CDS             793..1563
                     /locus_tag="JD016_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HAU5000503.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            1560..2336
                     /locus_tag="JD016_12745"
     CDS             1560..2336
                     /locus_tag="JD016_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HAU5000504.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            2391..3083
                     /locus_tag="JD016_12750"
     CDS             2391..3083
                     /locus_tag="JD016_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000505.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            3102..4526
                     /locus_tag="JD016_12755"
     CDS             3102..4526
                     /locus_tag="JD016_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU5000506.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(4766..7234)
                     /locus_tag="JD016_12760"
     CDS             complement(4766..7234)
                     /locus_tag="JD016_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000507.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSLISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(7237..7905)
                     /locus_tag="JD016_12765"
     CDS             complement(7237..7905)
                     /locus_tag="JD016_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000508.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            complement(8191..8718)
                     /locus_tag="JD016_12770"
     CDS             complement(8191..8718)
                     /locus_tag="JD016_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAU5000509.1"
                     /translation="MKSFANNITFSHIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIIAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            complement(8935..10077)
                     /locus_tag="JD016_12775"
     CDS             complement(8935..10077)
                     /locus_tag="JD016_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU5000510.1"
                     /translation="MSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQDKYLKAP
                     INTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYKVKLKTP
                     IEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTMINDRKQ
                     LNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIEYYVENM
                     SNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKNQINNINLFFDENII
                     LRVVENIISNAFRYAKNTVSILIYLEQELLTFVIEDDGIGFSKESLKLALRPFYRDKT
                     LNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            complement(10083..10757)
                     /locus_tag="JD016_12780"
     CDS             complement(10083..10757)
                     /locus_tag="JD016_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000511.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
BASE COUNT         3633 a         1557 c         1736 g         3926 t
ORIGIN      
        1 aaaattagta ggtgttatag aattgttatt ttttcatgtt agtattctct tagtaacaga
       61 tttaaggtat tacaagaagg gagaaattaa tatgaaagat ttaattcttg aaacaaaaaa
      121 cttatcaaaa agatatggag aacaaatggc agttaacaat gtttctctac aaattgaacg
      181 aaatagtata tatggacttt taggaccaaa tggagcagga aagtccacaa cacttaaaat
      241 gcttgttggt cttcttcgtc caacaaatgg tcgtattatt tttgatagaa agccttggaa
      301 acgagaatct cttgcaaaaa taggttcttt aatagaatcc cctgcattgt atggaaacct
      361 tacagcagag gaaaatttgt tagtacatac aaaaatattg ggaataccta aagataaaat
      421 ttatgaagtg ttggaaatag taaatttgaa aaatacaggt aaaaagagag cttcacaatt
      481 ttctatgggt atgaaacaaa ggttaggaat agctattgca cttttgagtg acccagagtt
      541 gttaatacta gatgaaccaa ccaatggtct tgaccccttt ggcattcaag aattgcgtga
      601 attgattgct tcttttccaa aaaaaggtat aactgtaatt ctttctagcc atattctatc
      661 agaggttgct caagttgtag ataatatagg aattataaat ggaggacgac tattatttca
      721 aggaatgcct gaccctaatg aaaatctaga agaatttttt acagatgtta ttcttaaagg
      781 aggacaaaac agatgaatgc tttacaatca gaacttttaa agtataaaag aacatttatg
      841 ggtaaactta ttgttttttt cccagtgtct tttgcagcat atgcatttat aatgcaatca
      901 acactcatgc aaaatccatt atctcaaaca acttcttggg cgtggcaaaa tcttttagca
      961 ttaattttta actggtggtc atttttattt ctgcctattg gatttgcatt gtttgctact
     1021 ttggtagctt tccaggaaaa aaaagctgga aattatcgtg ctttacgtac acacaatgtg
     1081 tctcctatga cactttggat taataaagtt atagctatgg cagtctacag ttttatttca
     1141 acgctagttt taatagtcgt aacaattata actggtttaa tattgaaagc tggtccagtt
     1201 ccttttggac agattatagg ggcaagtatt gtatgctggg ttgtttctct tgctatactt
     1261 ccacttcagc tatggctagc tacatggaaa ggtatgttcc taagtatggg agttggagct
     1321 ttaggtatga tatttggtgt ccttgctgcg acaaaaccat tttggattgc agtaccttgg
     1381 agttgggcag ttagaatggt atgcccaatc atcaacatac atcctaacgg tactatatta
     1441 gaagctggag acccactttt gaatacttca gtgattccta ttgggattgt tgtttctttg
     1501 gttgtgttta tagtattaac agcacttact gcagcttggt tcaataggag ggatgacaaa
     1561 tgataaagct attatcatca gaatggcttc gagcaaagag gacagctgtc cagtggctta
     1621 ctctttgtat gcctattatt ttttcattat gtgtggtttt ttatctcaca actaaatctg
     1681 gaagtacaca agaatttgct tttgaaggat tttttacagt atggactgta ttcatcattc
     1741 ctattggagt aggtatttta gcaggattta tagtgcagga ggaagagctt gctggaaatt
     1801 ttaatggttt tttatgtgta ggtatttctc gtgttagatt atatctggga aaatttcttt
     1861 ttcttctgtt ttgcctaact atatgcacat ttattgctac gttgatactt tgcataggaa
     1921 tgagtattgc agtgccatct ggagcaacaa tatggttatt cctgtcagca gcaggacttg
     1981 tggtgattgg gacacttcca ctattagcta ttcatctttg ggtgagtttt gcatggggta
     2041 tgggtgcatc tattggtatt agtatcggtg gaattttgat ggcagcaata cttggattaa
     2101 ctagcgttgg tgaaaaagta tgggcatttg ttccatggac atggccagta aaactaggta
     2161 tgcttccagg aatttatttt attaaagaag ctggaactat ttcaactgag gcattttact
     2221 ctaaagtgat gcaaacagct agtatagagc ttatagtagt agcaataggt ttgattatat
     2281 tcttgatagg aggcgttatt tggtttaaaa tgtgggaggg aaggaaaagt tattagtaga
     2341 cgaatattgt tttaatactg accttcccat aagagaaagg tggtgttata ttgagtaaaa
     2401 tattgattat tgatgatgaa atggacctag taatgctact tgaggatgaa ttgaaggcaa
     2461 aaggacatga agtgttgata gcttatgatg gtcaggcagg tatagagtta tcaaaacagg
     2521 agcctgacct tattatactg gatattatga tgcctaaaat gaatggattt gaggtatgtc
     2581 aggcaattag agatgatgtt ttatgcccaa ttatattttt aagtgcaaag caatcagaaa
     2641 ccgacaaaat caaagggttg acctttggag gggatgatta tgtaacaaag ccatttggac
     2701 tgcgtgaact tatggcaagg attgaagcta atctgcgtag ggaaaagcgt tcacagtata
     2761 tcaatgaaga gaacaagaga tctactttgt attttgggaa gttatgctta tatatgaagg
     2821 aacgtgcaat taaaattgat ggtaagaata ttgatttaac aaaaatagag tatgatattg
     2881 ttgaattact agcactccat gcaggacaag ttttctcaag agaacaaatc tatgaaaaag
     2941 tatgggggtg tgattctgaa ggtgattcct caactgttgt tgaacgagta aaaaaaatac
     3001 gagctaagtt ttctgctgtg acaccagaaa aagaatatat ttccacagtt tggggtattg
     3061 ggtataagtg gaataaaatg taaagagaga gaggttatta aatgaaaaat caatccctta
     3121 ttacccaatt tagacatact tttattttta ttataatagc cagtatagtt gccacagtga
     3181 ttacttatgt atttgctctc tatttgtata ttcattcctt aaataaggat atctatccac
     3241 caaactatta tgagcgacaa gttcctagaa ttgaaaaata catcaatgaa aaaaacataa
     3301 ctttgttatc acaatcgaat gaagaaggtt taaaaagaac gattcgtggg gatgatatgc
     3361 tatatcaagt tgtggataat aatggcaata ttttatatgg tactaacccc aaaaaactat
     3421 ttaaaacaaa agaagaatta tttaataatt ttatcaataa aactgttcgt aaaggtggtt
     3481 atatccatac tgttccaatc aaaggggata atggaaagat tgagggtgct gttattctat
     3541 tctaccaagt gaaaatcacc tttgcaaata ttagagggag atttgtattt gcagttatta
     3601 taatggctct gttttctcca tttctatata tcgtgggttt tactagatgg ctttctaagc
     3661 gctttgtaaa gaatatcaat caaccattac atctattgat tgatgcgtcc aagaagataa
     3721 aagaaaagga ccttgatttt gaaatcgact attattcaga taatgaactc ggtaaacttt
     3781 gtagtgcatt ttctgaaatg aaagacgaat taaaaggctc tttatctgca cagtggaaga
     3841 tggagcagga acgtgttgaa atggtggaag ctctagcaca tgatttaaaa tctcctcttt
     3901 ctattatttt gggatataca gatgcactta ttggtaataa tacagatgac aatgaaaaac
     3961 ttcataggta tcttacagtg ataagagaaa atacagaaaa aagtgctgct cttgtccaaa
     4021 agatgcaata tacgtctgac ttagaaaagt caaatataca attaaatctt gttcctataa
     4081 atttacctga atttttgagg caaaaggtgc aagactatga attacaggct catcaaaagg
     4141 aagttgaact tatattgaaa atgcagggga atatacaatc cccaattcaa atcgacgttg
     4201 ataggttgac acgaattttt gataatatca tatccaacag tctgcaatac actccatcag
     4261 gtggtaatat tagtattact gtaaaggatg aaaaaaattg tatttcttat gagatttgtg
     4321 attctggtag agggtttagc tctaaagatt taaaaaaggc tcttgataaa ttttatcgag
     4381 gagatgaagc taggcagaca aaaggtggac attctggtct tgggctttac attgtcaaac
     4441 aattagtgga acagcttgga ggttctgtta aaatagaaaa ttcaaaatca ggtggagcat
     4501 gtgtgaaatt ttggcatagc atttaactat atattttaaa ttaaaggaga gtgtctcaaa
     4561 gcgaaatttt taattcgtga ggcactcttt ttttgtgaag ttaaatatat tttcattatt
     4621 tcgacaatga aaaaaggctt atctctattt tgagacaggc cttttttaaa gcttatatta
     4681 tagttaaaaa gatttattag ttagatttga aagaaaatag agacagtcat tgataattta
     4741 atttatatta ttatctgtaa aatagttata aattgatatc tatacagtca attatattca
     4801 tctttttaac ttttcttaac ggaataagag tagctataat acatgcaagt acagaaataa
     4861 tagtagcttg taaaattgaa gtaattggta tattagtgaa atcaagcttt tctatagttg
     4921 ccatgtttat gataatagca ctggcatacc cagcaattga accaaatata gcagcaaaaa
     4981 ttccataata aacaccttcc cataaaaaca ttttatataa gctactatta ctcataccaa
     5041 tagctctttt aactccaatc tcattagtcc tagtatttat atttgtatga gttgtgttaa
     5101 tgatgtttaa agtaccaatc aatcccacaa ataatataac tgcccatgct agaaattcta
     5161 tttgtttaaa actctcttta agttgtttat ctgattcaat atatgatatc caatgagagc
     5221 caggattatt ttcacaaatc tgttcaatag acaatcttat ttgttctaat tttgatttat
     5281 ctttagcata aatatttatc tggttgaact tgtttttatt tgtcaaggtg ttataaacag
     5341 aatcatatac aataacatca acaccattta tccagccatt accttgtaaa aagaacattt
     5401 tatctactgt actatatata tctagttgtt ttttgttgat tgtgatacta tctttaggtt
     5461 ttaaatttgt atgtctagtt tttacacctg gtgtagaaat agcaatcgga ttttttataa
     5521 aacagataga accattttta aagtcttcta gtatagaatc tgtaatagaa ggcattaagt
     5581 cttttaaagt ttgctcatca attccaatga tttttaacat ctcaccacta ttttcaaaat
     5641 ttatatctgt atctatttcc ccctgtttgt attcagagta ctttataaag gaagtgcgat
     5701 tgacgttttt cattttagct attttatcta gtgttgattt atcaaaacca gatatttcat
     5761 ttgttaagga aaaatctcct tcttttaaat tacttactga cctagataca tctaatacat
     5821 tagagaactc actcagtgca acgaatactg ttatactcat aaataatgat agtatagtta
     5881 ttatagttct tcctttattt ctttttaagt ttagccatgc tagatgtctt tcaaagtttt
     5941 ttattgtttt ttctctttta atttttcttt tgattttggt ggtactacca accatagcaa
     6001 ttttgggaga gacatgtgaa gcatatattg ctgatggtaa tgctgaaata agtgaaaaaa
     6061 tcaaagatac attagtgctt aatactaatg ggaacatata tgctgttgta tttttatgta
     6121 taagctcagt aatttcttta ttatcatttg ctagaagtat atctggattt aagatattag
     6181 tgaccattcc agttatgcct ttcgaagata taattccaat tacagctcca ataggtatgg
     6241 caacagtaca taaaattaag atttgtaaaa taactatctt atatatctgg tttggttcag
     6301 ctcctattgc tcttaaacat ccatactctt taattttctt tgtaacagag atttttaaaa
     6361 tattatagat tacaagtcca gaagccagta aaaccaataa ggcaacaaat aaagatacca
     6421 atataatcat agatatacca tcactattag aactattttc atctttttca aactgtatct
     6481 taagagcatt taagtaaatc caattatatt gaattgaatt gtgagaaaga tttatttttt
     6541 tagctaattg atttactatt tcttgaaact tttcttgctg ttttatttta aaatccaaag
     6601 aggataaaat atatctctta ggtaatagat tttctgatgt acctttacct acaataccat
     6661 ttactatacc acttacatat cctgtataat catcctctaa aatgccactc aatttaaagt
     6721 tagctgtgta attatatggt ggtatggtat catttaaaag tgaaatatct aaattcatag
     6781 gtatagtcac tcctagtctt ggttttatac ccataagttt tagtgtgttt tcatctaagg
     6841 ctatttcgtt tttatcttta ggcaaatgtc cagattttaa tttcattaat tttgggtagt
     6901 tagaaagtcc tgttttatca tactctttta caagtacaga gataccagaa tcctttattt
     6961 tactactgcc aatagttatt gaagtaccag cttgataaac tctatcatct ttctttatgt
     7021 catcaacttg atttttagtg agttggtgaa aggatacatg tctatcacca ttaaaagaac
     7081 gggcttgttc aattcttaaa ttttgtagga taccaattga ctgtccaacc acagttgtca
     7141 taatagttga tagtactatt gctataatga tgaatgtagt cataagtttc tggcttttta
     7201 attcttttat tgaaagtgaa aggtaattct tcatttctat ttcacctcca taagaacacc
     7261 atcaataatt gaaatttttc tatcagcaag ttttgcaatt ctatcatcat gagtaattac
     7321 cactaaagtc tttccaaact gttttgaagt ttttataaga agattcataa cttcactgcc
     7381 acttttactg tccaagtttc ctgtaggctc atcagcaaat attatatctg gttttgcaat
     7441 aagtgctcta gcaattgcaa ctctttgttg ctgaccgcct gaaagttcat ggggaagatg
     7501 attgagtcta tctgcaattc caagtaatga tgaaatttca tttaaatagt ctttatcagg
     7561 ttgtttttta tctagaagta caggcattga aatattttca gatgctgtaa gaacaggtaa
     7621 aagattaaat tgttgaaaga cgaatccaaa ggattttctt ctaagtgcag atagttcttt
     7681 ttcagtgtgt ttatatatat ctttatcatc aagtaacact tgtccagagg taggattatc
     7741 taatccactt aaaatatgta aaagtgtgct ttttccactt ccagaaggtc caattacaga
     7801 aacaaattca cctgagttta tttctaaatt tacattatta agtgctataa ctttattgtt
     7861 attagaacca tatatttttg ataattgttt tgcttttata atcataaaaa acactctcct
     7921 tttctatata agaagagtgt aagttttaaa actcaatttt ttcccaactt ttagcttaaa
     7981 tctaaaaagt cttcactatt tgcggataat tcgcagtgct gataagcagt ttcagtatca
     8041 tttttaattt aaaatgtaat ttatgcacta aaggctttac aatacaactc tattgcttta
     8101 aaactatttt acacataaac ttgtggataa ttttttatgt tttacttcct cattattata
     8161 taaattattt ttgtcacaaa ttctaagttt tcacataaat atacttttta tatcacgatt
     8221 taattctttt gattttatca taattgctat ttcttcaaaa gaagaaagaa aagccacgat
     8281 acatatcata attcctgcaa catcaagacc aaagcagata tagagtaatg gaaacgcaaa
     8341 gatagaaatt ccagtgattt tattcatcaa agtatgaagc gatgaaaatg tgcgatactt
     8401 aaaaaaacca atactgtaac taatcaaccg taacaatgct attgcaataa tacaccacca
     8461 tagatacatg ggaagagcaa tattttttcc gacagtaacc aaaattgtaa ccgcaaaaat
     8521 gaaatcagca atactatcta actttgcacc tattccactt tgttgtctca actttcgggc
     8581 aataagacca tcaagtatat cactaattcc acaccaaaaa taacatatcc agaaaggcag
     8641 tgaacagaat gtagaactaa gcattagcaa tgcaaacaca atatgagaga atgtaatatt
     8701 attagcaaaa cttttaatat aatcacctcc aaaattctaa tttcatacta ttctacatat
     8761 ttaaatatat atttctatta ttgtttcaaa tgccttttgc caaaatatta taattgaagc
     8821 aactcttaca acttctacaa aatagtatat atattggttg taaattgaaa tacattttat
     8881 aaattctaaa ctaacaaagc ttatataaga acagtgaaag cttacaaaat cattttaatt
     8941 tcttgttgag aatttagcag taattttagc actgttagta gaattatttt caatagaaat
     9001 agagccacca tgtttttcgc ataatatttt tgagatataa agacccatac caaagtgtgc
     9061 attgctgtca tttaaagtct tatctctata gaaaggtctt aaagctaatt ttagagattc
     9121 cttactaaat cctataccat catcttcaat tacaaaggta aggagttctt gttcaagata
     9181 tataagtatg gatacagtat ttttagcata tctaaaagca ttcgatatta tattttcgac
     9241 gactctaaga atgatatttt catcaaaaaa tagatttata ttatttattt gatttttaag
     9301 tgtaaaagat tttccttctt gtttagcaat gatagatata ttttcatcta agttttctat
     9361 aaacttgttt atattcgaca tagattttga aacaactaaa tcttctaatc gttgagcatt
     9421 actcatattt tctacatagt attcaattct gttaatatgc tcagacataa gttgtgtggt
     9481 tgataaaatc ttctcatcac taagcttacc tgttggtatg tattttgtta aatagtttga
     9541 ataacctttt agaactgtta ggggatttct taaatcatga gaaaaagcag cattaagttg
     9601 ttttctatca ttaatcatag tccacatctt tatgttgttt ttatttagtt cagacctcat
     9661 tttttcaaaa gcattacata gattacctaa ttcatcatta ttattatatt ctaagcaaaa
     9721 gtctaaattg ttgttggata tttccattgc acctttattg agtatttcta tgggtgtttt
     9781 taattttacc ttgtaaaaaa ttatatcagc taataataaa agtcctataa aaaatataag
     9841 tggaagtaca agctgaagta tgttgattat attaagtaat gttttatcat aatcactaaa
     9901 ttggttcaca ttgttttgaa ctatggttat aatacctgta tttataggtg cttttaaata
     9961 cttatcttgt atagaattat atagattatt aagtaaaatg tatgcgaaag tagtaagcac
    10021 tgatgctata attagtgaag ataaagtaag aacaaagaaa gcttttttta atgacatatt
    10081 atttatttta tccatttata accaactccc cagactgttt ctatataatt ttcaagagta
    10141 tgttttgata gtttgattcg tatttttcta atgtgttcca ttataacatc actatttcca
    10201 ttgctattgt atccccagac tctatcatat attttttctt tatcaaatac ctgtccagga
    10261 ttcatagaga gaagctctac tatatcaaat tccttttttg aaagtgaaat ctgattatta
    10321 tatatacaaa ttaacctttt ttcgtaatct attgttaaat cattataaaa tttaatttta
    10381 gtttgatttg acttcctttg ttcacgtctt aaatgggcag atactctagc tccaagttcg
    10441 tctaaatcaa aaggttttac aatataatca tcagcaccag cacgaaaacc atttatcttg
    10501 tcagtatttt caattctagc tgtaagaaat agaatagggc atgatacata atctctaatt
    10561 ctattacaaa cttcaattcc atctatttca ggcatgttta tatcaagtag aatgatatct
    10621 ggatttttag atattttttt taatgcgtct tttccacttt gagcagtgta aacctcataa
    10681 ccattcattt caaagtaatc ttttaagagt gttattatac cttcctcatc atctacgacg
    10741 agtattttat tgttcataag aatgctccct tctaaattaa gtttgtatag tatgtatatt
    10801 ttagtataaa aatctcaata aattatcaaa ttcttagtta tatttcaaaa ct
//
LOCUS       DACXMZ010000131        10806 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.132, whole genome shotgun sequence.
ACCESSION   DACXMZ010000131 DACXMZ010000000
VERSION     DACXMZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.132"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            101..2221
                     /locus_tag="JD016_12785"
     CDS             101..2221
                     /locus_tag="JD016_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAU5000512.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRLFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            2325..3579
                     /locus_tag="JD016_12790"
                     /pseudo
     CDS             2325..3579
                     /locus_tag="JD016_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
     gene            complement(3733..4794)
                     /gene="ytvI"
                     /locus_tag="JD016_12795"
     CDS             complement(3733..4794)
                     /gene="ytvI"
                     /locus_tag="JD016_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HAU5000513.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            5068..5808
                     /locus_tag="JD016_12800"
     CDS             5068..5808
                     /locus_tag="JD016_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HAU5000514.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            5820..7598
                     /locus_tag="JD016_12805"
     CDS             5820..7598
                     /locus_tag="JD016_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAU5000515.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            7765..8682
                     /gene="pfkB"
                     /locus_tag="JD016_12810"
     CDS             7765..8682
                     /gene="pfkB"
                     /locus_tag="JD016_12810"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAU5000516.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKKF"
     gene            8719..10638
                     /locus_tag="JD016_12815"
     CDS             8719..10638
                     /locus_tag="JD016_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAU5000517.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         4489 a         1052 c         1894 g         3371 t
ORIGIN      
        1 aattatggta atattatagt tacgatatta agaaaagttc tatttagtta gattttaagc
       61 gtaatatgag attatgtata gagaaaggat gtatttttag atgggaaaaa tattagtgct
      121 agcagaaaaa ccttctgttg gaagagatat agcaaaagtt ttagggtcaa aaaatgaaaa
      181 aaatggttat atagaggggc caaagtatgt aataacttgg gcactaggtc atttggttac
      241 acttgctgac cccgaaagtt atggagaaag atataaatca tggagcttag aagacctgcc
      301 aatacttccc aagcacctta agactgttgt aataaaaaag agtggaaagc agtttaatac
      361 agttaaatcg caaatgaata gaaatgatat agatgaaata gttatagcaa cagatgcagg
      421 tcgtgaagga gaacttgtag caaggtggat aatagaaaaa tcacaggtaa aaaaacctat
      481 aaaacgtctt tggatttctt caagtacaga caaagcaata aaagacggat ttgcaaaatt
      541 aagaagtgga aaagaatatg aaaatttgta ttactcagca atagcacgag cagaagctga
      601 ttggattatt ggaataaatg caaccagagc tttgacaaca aagtacaatg cacagctttc
      661 ttgtggaaga gtacaaactc ctactcttgc aatgttgctt aaaagagaag aagaaataag
      721 aaatttcaaa cctaaagagt attatggatt agaattaatt gctacaaagg gaaattcaga
      781 tataaaattt atttggaatg ataaaaacaa taattcttca acttttagta aagaaaagat
      841 agaatccaca ttaaaaaaag taaaaggtgt agacataaaa atatctgaag taaacaagtc
      901 atataagaaa aaatattcac ctgcactcta tgatttaaca gaattacaaa gagatgcaaa
      961 taaaatgttt ggattttcag caaaagaaac tttatctata atgcaaaaac tgtatgaaca
     1021 tcataaagta cttacttatc ctagaactga ttctagatat ttaacatcag atattgttgg
     1081 aacattaaaa gatagaataa aagctgtaaa tattagtgat tattcaaaga tttgtaatag
     1141 attattaaaa agcaatatta tagctaataa atcgtttgtt gacaattcaa aggtaacaga
     1201 ccaccatgca ataataccaa cagaacagaa ggtattcatg agtgatttta gtgataaaga
     1261 gcgaaaaata tataatcttg tagtaaaaag atttttagca gtactatctc ctccttttga
     1321 atatgagcag acaactataa gtgcaactgt agaaggagaa atatttaagg ctaagggaaa
     1381 caaagtaata aatttaggtt ggaaagaaag ttataaagat attgaagatg aagatgagta
     1441 tgaaagtata ccaaatgttt tagaaaaaga tgtacttaag gtcaagggat taaagataac
     1501 ttcaggaaaa acaaatccac caccatactt aaatgaggga acattattga ctgctatgga
     1561 aaagaatggt ctagggacag ttgctacaag agcagatata atagaaaaat tattcaattc
     1621 atttttaatg gaaaagaaaa ataaagatat atatataaca tcaaaaggaa gacaattatt
     1681 agatttagtt ccagtagact taaagacacc agaacttaca gcttcatggg aaaagaaatt
     1741 attggatatt tcaaagggaa aacttagtaa aaatacattc ataaatgaca tgaaagatta
     1801 ttcaaaagat attgtactag aagtaaaaaa tagtgataat aaatttaaac atgataactt
     1861 aactaaaaat agatgtccag aatgtggtaa atttatgtta gaggtaaatg gtaaaaaagg
     1921 taagatgttg atttgtgaag atagagagtg taatactaga aagactatat cacaagtaac
     1981 caattcaaga tgtcctaact gccataaaaa attagaactt cgtggagaag gtgaggggag
     2041 actcttcact tgtagttgcg gatacagaga aaagttgtct tctttcaaca agagaaagca
     2101 agaagaaaaa cagaaggcat caaaaaaaga tgttaatcaa tatttaaaaa atcaaaataa
     2161 aaatcaagag acatttaata atccatttgc agaagcatta gctaagctaa aaaacaaata
     2221 attttaaagt tattattata cacaattata actttaactg tatattagtt atatgtactg
     2281 atataatata gtgtatagtt taggcatgaa agtagggctg ttaaatggat ttaagtggca
     2341 tatttaaata ctattgtaaa gagtgtgaaa atacatggaa taattcgagt gttgaattat
     2401 ttgagaatat agaaacgtat agtaaagatt cacaaaaaaa gagggaaaaa gaattagata
     2461 aattgctaaa tacaatatca gttcatttag agaggtatcc aagtgatgct gtattgagaa
     2521 aaatgtgggt aaaaaagggc gaggttttct tacaaaagac attggaaaaa gaaaatattt
     2581 ttaagttaga aaaaatggat gtagaggata gaaaaaaatt tttagatata acaaaacagt
     2641 ttattagaga tgctagaaaa tttgatgatg atttacctat aggtgatatt atgcaagcta
     2701 tgaggaatgt atggatttca aatgcattac aattattatt tggtaaagaa gtatattatt
     2761 caaaagctaa ctttgcatat agtatgttat atccatatac tgataattat ttagacaata
     2821 caaatataga taaaaatgat aagattttat ttaataactg gttagaaaaa aggctcctgg
     2881 gagaacacat taaatctaag gattatcatg aaagtaaagt atctcaaatg atagattata
     2941 ttgaaagtgt ataccctaga gaaaagttta cagaagttta tgaatcgtta ttattaatat
     3001 ttaaaagtca agtaaatagt ttaaaacaac atggtaagga aaatcatttg tgtaaagaag
     3061 atttattatc catttctatt gaaaaaggag gttcatccgt tttagtagat ggatatttaa
     3121 taagtggatt gatgacaaag gaagaaatag agttttgtat aggatatgga tttttattac
     3181 aaatatctga tgatttacaa gatataaaag aagatttaaa atacaaccat aaaactatta
     3241 ttacagagat gtcaaaagag ggtactttag ataaagttgt aaataaacta ataaatttta
     3301 ctattgagtt aatagatagt tttaaaatta ataataaaaa taaatctgta atagctatga
     3361 taaagaatga ttgcttaatg ttaattttat tttctgtagt ttataatgct gaattttttt
     3421 ctgtaggata tataaaagaa gtagagaaat ttattccata tacaatagat tattcattag
     3481 agattgaaga aaaaaataaa agaaaaattt aaaaatatag atgttttaaa taatgaaaat
     3541 gaatataaag aaatgattga tattatttgt gcagagtaga aaaaaaggga gtgtcttaga
     3601 atagactttt tagtttatga ggcactcttt ttgtataaaa tcaaatgtat tttcatcatt
     3661 ttaacaatta aaaagagctt atccctattt gagaaagccc tttttaattt aaataaaaat
     3721 gtcaaggtta aactatttaa atgcagtaat aatcccatta tcatgtaagt tttttatgat
     3781 tgtagctgcg atagggaatc caaataatcc agctattcca aatagttgag ctccaacaaa
     3841 catacacatt aatgttatta aaggatatag gccaatttgg tgtcctacta cctttggttc
     3901 taatatttgt ctaacaacgg ttataattat gtatagtatt aaaagtccta ttgctaaatt
     3961 aatatttcca ttaatgagtg agataatcat ccaaggtgtc aaaatacttc ctgtacccaa
     4021 gactggcaaa atatcaacaa gtgctatgat gacagatata gtaaatgcat tttcaacctt
     4081 taggatagta agtccaataa gtagttctat gaatgtcact gatagtagta ttgcataggc
     4141 ttttagtagt tttaaaagag cattaatact gctatttttt atatttatta tcataaattg
     4201 acttttttct ggaaattgtt ttaagataaa gtttactatt ttttgatagt caaaagtaaa
     4261 gaaaaaagaa gcaattaaag taataataag ttttaacata aatgcaggtg ttttacttgc
     4321 aatactagca atagcattga gtgcatttgt tgaaatagaa gctactaaag acatcataga
     4381 ttgacttata ttatcccaac caagtattaa tgctaggtta acattaggtg tactagcatc
     4441 tatctttgaa aataaagtat ttagcgttgg ttgtatactt gatttatata tttcaggaag
     4501 tctaaaaaat acatcactta cacttgcaaa tatcttcaca ccaaagctaa ctaataaaaa
     4561 tatacacaac ccataaaaaa ttaaaagtat tactatagaa ataagtgtac gatttaaatt
     4621 tgtattattt gtaataagtc ttataatagg tcttaagatg gatgcaatga taaaactgat
     4681 tacaaaaggc attaataatg gcaataagaa ttttaataca atatatacaa tacttgttat
     4741 taaaactaca tatgtaaatt taattataaa attcttttgc ttttgaatat ccataatata
     4801 aaacctccaa tgtatttgat attattctaa atatggtctt ttttacagaa aatatactac
     4861 tgtatattga ttataaacta taaagcttat gtaaagtaaa taaattatgt aaaatttagg
     4921 ttaattttgt ataaagagat acttttatat tttacaatta ataaccaaat actaaaaaat
     4981 attttaaata aagaacttaa aattgtgata aaattaattt atagatatta aaaaaagtat
     5041 aatatatggt ttttaagagg tgctaagatg aaaaatttta gagatttagg tgggaacaaa
     5101 actgaggatg gcagaactgt aaagaaggga ttattttata ggtctgcaaa attatcaaat
     5161 ctaagtgaaa atgatataaa gattttaaaa gagttaaata ttaaatatat atttgattat
     5221 agaagtgatg aagaagctcg taaacatcca tcaacaataa tatcaaatat aaaaaatata
     5281 agaataccag caatgagaga gctagaagaa tcaggaggaa gttttggttc tattgaagat
     5341 atgattgatg gattgtttga aaaagatggt gcatttaata tgttaaataa tagttactat
     5401 aatttgccta taaataatcc gtcatataaa aaattagtag aacttataag agactattct
     5461 aatttaccaa tattaaatca ttgtacagct ggtaaggata gaactggtgt ggggagtgct
     5521 ataattctta tgattttagg ggtatcgaga gagaatataa tgaaagatta tctaaaaagt
     5581 aatgattttg cagataaaga gatagaaaga tttatagaat ataagcctaa atttaaggac
     5641 attccaaaag aaaatttgaa atatatattt ggtgtaaatg aagagtatat gaaaactgca
     5701 tttagaagaa ttgatgaaga atacattagt gtagaagcat atttatatgg agaatttaat
     5761 ttaaataaag aagaaataag aaaattaaga aatcaatatc tagagtaaaa ggtgagttta
     5821 tgagtttaaa tataacagca agacagataa atataataaa aatattgtta aattcaaaag
     5881 atacaattag tggtattgcc ttaagtcaag agataggatg ttcttcaaaa actatacaaa
     5941 atgaaataaa ggatattaat aaacaactta gaaatggtaa aattttgtca ataagaggag
     6001 ttggttataa actagaagga agttttgatg aggtaaatct aaatagcaat ctctatgatg
     6061 atgtagatag agtagaatat ataatcagaa aaatattaac cttaagtaat gaagaaaaaa
     6121 atactataaa acttgaagat ttagccgatt ctatgtatgt aagtgtttca acagtaaaaa
     6181 atgatttaaa agaagtaaaa agaatgttgg aaaagtattc aatatcagtt aaatctaagc
     6241 ataaacaagg tatatgtgtt ttagaaagtg aagataagat attaaattgt atagttgact
     6301 taagcaataa gagagataat caattgagtt taaatgattt tttaaatgat gatataaagg
     6361 aaagaaaact attaataaaa aaattattat tagacatatt aaatgaaaat ggattagtat
     6421 taactgatat agaatttaag agtgtgttaa ataatatatt agttatgtta agcagacata
     6481 agtataatga aaaagaattt atagagaatt atatagaaaa ttataggagc aaaagaagct
     6541 caattttaga aaatgaacaa aacaaagaaa ttataataaa ttcaataaaa acattttgta
     6601 aaaatttaaa attagctaca tcaatagata taagtaatga taaggtattt gaagagtttt
     6661 tatataagca tattagtagt ttatgtaaaa aaataaagtt aggtataaat caatcaccta
     6721 taatggctca agatataaaa ataaaatatc catttgcttt tgaactagct aatatagcta
     6781 aaaagacaat agaaaaagat ttgaagatag aaattaatga agatgaagtt gcaaacatag
     6841 ctctacatat aggaggagca gttgaaagag catcttataa taataaagat aaggttttca
     6901 aaactataat agtttgtact tcaggcatag gcacatcaat gttaataaaa gctaaattag
     6961 agaatatatt taaggaaaaa ttagaaatac taaagattat tccttcatac ttagttgaat
     7021 atataaaagt tatagatgtt gattttgtaa tatctactgt tcctattgaa gtaggagatg
     7081 tgccagtaat aaatatatct ccaattttaa gtgagaaaga aattagatta attgaaaaat
     7141 atatagaaac aggaaatata tacctagatt tagatataaa aagcctgttt gatagtgaat
     7201 tgttttttac tgatttggtg tttaaaagga aagaagaagt tatagatttt atggcaagta
     7261 aacttgtaca aaagggctat atagatgagg atatgaaaaa atcttacttt gagagagaaa
     7321 aaatagcaac aacagaaata gggaatatgg taactatacc acatggagct attggaaaaa
     7381 tttttgataa taaaatagca attggtatat taaagaagcc tatttcttgg gaaatatcag
     7441 atgttcgttt aataataatg ttagcccttg ataaagataa aatattagat tatgaagtaa
     7501 tattttcaaa aatatataaa agagtagatt ctatagcaaa agttataagt atatgtgaaa
     7561 atagtagttt tgaaaaattt gtaaatttat ttaattagat gggtagtatt gtatatacac
     7621 ctgtaaattt ggataatata aaactaaata agataaaaac gataaatttc tcttttaatt
     7681 aagagaaatt ttttgacttt caaggatatt aatataaagt taagatataa acataaagca
     7741 aataaataaa aaagaggtgg caatatgatt tatacagtga cattaaatcc atcaatagat
     7801 tatatagtta agctagatga gctaaagaca ggaagtacaa atcgtgtaaa agaagaatac
     7861 gtttatccag gaggaaaagg tataaatgta tctcgcatac ttaaggaact tggaaatgat
     7921 aatacatctt taggttttat aagtggtttt actggagaat atataataag aacattggaa
     7981 gaaaaagaat tgaagacaga ttttataaag ataaaaagtg gtttttcaag aataaatgta
     8041 aaaataaagg cgttaaagga aactgagatt aatggacaag gtccaaatat agatgatgaa
     8101 gacattgata ctttatataa aaaacttgat aagctaaatc aagatgatat tttaatattg
     8161 gcaggaagta taccatctac tttagatgaa aaactatacg aaaatataat ggctagatta
     8221 gaaaaaaaga atataaaagt tgtagtagat gcaactaaaa atcttcttct taatgtttta
     8281 aaatacaaac cttttctaat taagcctaat aacgacgaat tagaagaact atttggtgtt
     8341 aaattaaatt caattgaaga catggtaaaa tatgctagaa ggttaaagga aatgggagct
     8401 ataaatgtct tggtatcaat gggaaaagat ggagcattgt tgattacaga ggaagatgaa
     8461 gtgcttataa gtgatgttcc aaaaggaaaa gtaaaaaatt ctgtaggagc aggtgattca
     8521 atggtagcag gatttatatc tgggtatttg aatactggga aatatgatta tgctcttaaa
     8581 ttgggagcag cttcaggtag tgcaactgct ttttcatatg acttagccaa aagagaatac
     8641 attgataaat tggtaaatga aatatctgta aagaagtttt aaaatattaa aaaatatata
     8701 gaaaggtaat taatatttat gaaaattata gatttattag atgaaaagtc aattaagtta
     8761 aatttaaatt caaaaaccaa atcagaagca atagaagaat tagtagattt ggtcgcaaat
     8821 agtggaaatt taaatgataa agaaaactac aaaaaagcaa ttttggctcg tgaagaaatg
     8881 agtacaacag gaattggaga aggtgtagct atacctcatg ctaaaaattc ttcagttact
     8941 aaagcttgta tagcagcagc agtgtcaaaa gaaggtatag attatgaatc gtttgacgga
     9001 agtttatcaa atctattttt tatgatagct gcaccagacg gagctaacaa tactcatctt
     9061 gaagtattat caagattatc tacaatactt atggatgaag actttagaaa taaacttata
     9121 aattcaagct ctgaaaaaga atttaaggaa ataatagata aaaaggaaag agaaaaattc
     9181 agtgaagaat atcaagatga gaaagtggct gaaaaaaata taatagatac taaagagaat
     9241 gatgctaata agtatcctaa ggttttagca gtaacagcat gtccaactgg aatagcacat
     9301 acatttatgg cagctgaaag tttaaataaa atggcagaaa acaaaggtgt atcaataaaa
     9361 gtagaaacta atggttcagc aggtgttaaa aataagctta caaaagatga aatagaaaat
     9421 gcaacatgta taatagtggc agcagataaa aatgttgaga tggctagatt taatggcaaa
     9481 aaagtcataa aaactaaggt ggctgatggt atacacaaag cggaagaact tatagacaag
     9541 gctgtaaatg gagatgctcc aatttaccat ggaggagatg gaagtcacaa cgaatcaaat
     9601 gaagaaagtg aaagtggatt tagaaaagta tacaaacact taatgaatgg tgtatctaat
     9661 atgttaccat ttgttattgg tggagggata cttatagcga tagccttttt acttgatgat
     9721 tatactatta acccttcaaa ctttggttca aatacaccta tagcagcatt ttttaaagga
     9781 ataggagata aagcttttgg atttatgtta cctgtacttg ctggatatat agcatactct
     9841 atatctgata gaccagcgtt tgttgtagga tttgtaggag gtgctctagc aggtgatggt
     9901 ggttcaggct ttttaggtgc actacttgca ggttttatag caggttattt agtagaaggg
     9961 cttaagaaaa tatttagtgt actaccagct tcattggaag gaataaaacc agtattgtta
    10021 tatcctttac ttggaacact tttgatggga attataatga catttttagt aatacctcca
    10081 gttacagcaa ttaataatgc aatggttgga tttttaaatg gtttaggtgg aacttcaaaa
    10141 atagttttag gattagtatt aggtggaatg atggctgtgg atatgggtgg acctgtaaat
    10201 aaagcagcat atgtatttgg agttgcatca cttgaatcag ggcagtttga aataatggca
    10261 gcagtaatgg ctggtggtat ggttccgcca cttgctatag ctcttgcaac tacatttttt
    10321 aagaatagat ttactaaaga agaaagagat tctggtaaag ttaattatgt aatgggatta
    10381 tcatttgtta ctgaaggagc tatacctttt gcagctggag acccacttca tgttatacca
    10441 gcttgtgttg gaggttcagc agtggcagga gcacttagta tgttgttcaa cgcagctctt
    10501 cgtgcacctc atggaggagt atttgttata ccagtggtta cccatccatt tgcatatata
    10561 ttagctatag ctgtaggtgc tttggttgga atgatgcttt tagcattact taaaaaacca
    10621 ttaaatcaag aagtatagca attaaaggat attcaagaac ttaaaatatg atgttttata
    10681 aaaattaata tattgctgaa ttattttaaa ataaggattt tatgaggctg ttatataacg
    10741 atttgtaatt gttatcttga cagtctttta tttttatata agtaaaataa gtgtgttttg
    10801 ctttat
//
LOCUS       DACXMZ010000132        10738 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.133, whole genome shotgun sequence.
ACCESSION   DACXMZ010000132 DACXMZ010000000
VERSION     DACXMZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10738
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.133"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(138..470)
                     /locus_tag="JD016_12820"
     CDS             complement(138..470)
                     /locus_tag="JD016_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAU5000518.1"
                     /translation="MNTREMKEYRVFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(714..1355)
                     /locus_tag="JD016_12825"
     CDS             complement(714..1355)
                     /locus_tag="JD016_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAU5000519.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(1336..1632)
                     /locus_tag="JD016_12830"
     CDS             complement(1336..1632)
                     /locus_tag="JD016_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAU5000520.1"
                     /translation="MLGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATI
                     SYHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            2022..2081
                     /locus_tag="JD016_12835"
     CDS             2022..2081
                     /locus_tag="JD016_12835"
                     /inference="COORDINATES: protein motif:HMM:PF13333.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAU5000521.1"
                     /translation="MTEYIYYYNNKRIKKINLA"
     gene            complement(2252..2386)
                     /locus_tag="JD016_12840"
     CDS             complement(2252..2386)
                     /locus_tag="JD016_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000522.1"
                     /translation="MNISRRAMKIIELAQKIANKRGVTVQDAWNDAMKEYKEKYEYVA
                     "
     gene            complement(2597..3349)
                     /locus_tag="JD016_12845"
     CDS             complement(2597..3349)
                     /locus_tag="JD016_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAU5000523.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(3408..5063)
                     /locus_tag="JD016_12850"
     CDS             complement(3408..5063)
                     /locus_tag="JD016_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU5000524.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIYIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(5494..5831)
                     /locus_tag="JD016_12855"
                     /pseudo
     CDS             complement(5494..5831)
                     /locus_tag="JD016_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889974.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(6215..6298)
                     /locus_tag="JD016_12860"
                     /pseudo
     CDS             complement(6215..6298)
                     /locus_tag="JD016_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
     gene            complement(6336..7358)
                     /locus_tag="JD016_12865"
     CDS             complement(6336..7358)
                     /locus_tag="JD016_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAU5000525.1"
                     /translation="MRLIAPTIIRTSHKIEAPGIDRMEMVEIGGIQQALYIRGQNADN
                     PIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNVGKTYFANDPATVLQIMSAE
                     RVLQDVHEVTAYLKQQFNKEKIILIGHSWGTVLGTMAVQTYPEDYSAYIGIAQTVNMV
                     DNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGPSYLAALMTVRTYQSKYGLS
                     INMDLGNIISILATPYYSLRELKNTAIVNPNAIDNQGDVLRFLVEEADIRNYGVDYQV
                     PVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDADHLPFLSKKAEFDCVLLEEI
                     APKLAK"
     gene            complement(7499..8155)
                     /locus_tag="JD016_12870"
     CDS             complement(7499..8155)
                     /locus_tag="JD016_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAU5000526.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTIRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            8477..8683
                     /locus_tag="JD016_12875"
     CDS             8477..8683
                     /locus_tag="JD016_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000527.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCFAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(8836..9264)
                     /locus_tag="JD016_12880"
     CDS             complement(8836..9264)
                     /locus_tag="JD016_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAU5000528.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(9740..10681)
                     /locus_tag="JD016_12885"
     CDS             complement(9740..10681)
                     /locus_tag="JD016_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HAU5000529.1"
                     /translation="MMNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGK
                     NIVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNY
                     NNEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKKL
                     KNAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNS
                     NTTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAE
                     LYAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNGKR"
BASE COUNT         3569 a         1914 c         1129 g         4126 t
ORIGIN      
        1 actatattat ttaaaaattt atatttatat atgcaattct aaaaacttaa taagaagtga
       61 acttagaaaa taaagcttaa tttcaaagac ttaaatagac tattattatt agtctaaata
      121 aactacgtct atcaaaatta tatattaaat tcttctaaaa tttttattgc aaatcttgcg
      181 tttggatatg taatagaccc gccaccaatt acacctattt ttatagcttc tataatttca
      241 tctttacttg ctccagaatt aatacaaccc tcaatatgat aacaaataca ctcatcacat
      301 cggcttaaaa ttgaaatagc tagtcccatt aattccttat tttttttact tatagcccct
      361 tctgaataag tcttatcatc aagacttcca aatactctaa aaaactcatc tgtttcgctt
      421 aattttttat tgtaaaatac cctatattct ttcatttctc tagtattcat cttctacctc
      481 ctatgttttt cttataagaa tatcacactg tacttatatt ttcaatatta aatacaaatt
      541 ttcattttat gttcattagc ctaatttata ttattttttg aaatctcaat aagttatcat
      601 ataagtaaga ttttttcaga actaaaatag aaatcttagt aatttcttta ttatctatct
      661 cagttctgaa atatattatt cttatccaaa cattattata gtgattaatt atattagtta
      721 tttttacctg aaattttata catcaaataa gaatatataa atggtagtat tacaattaca
      781 aagattacaa agatattata aaaaggaata ttaataaatg cattaataat taataatatt
      841 ccaccaataa gccaaatcca tccagctaaa tggtgtgtct tattccaatt atcttcatca
      901 ttcagagtcc atggcaaaat tattcctact gagtaatttc gtttacattt aggaagatag
      961 tttccaaata aaataactat tattcctaaa attaatcctg tataaaaatt agactccaaa
     1021 ctaactcctg ttgaatataa aaccatcatt atttgtataa ttactgaaag tatagcaact
     1081 ccccattttg ataaattttg tataacattt gaaaaattag ctttcttagg ctcattctca
     1141 agtctaaacc atgtatacaa atggatacat agcattaaca atggtacacc aaaagctgct
     1201 ataacctttg gagcataatt atctggattt ccataattat caaaatgaat tgcaacttca
     1261 tttggtaatc tattataaaa atatattgat acaataaatg gtataaaaca tattaaagta
     1321 gataaaataa attgtttatt tatcgctttt ttcattatct ttttctccct tctccataaa
     1381 ttgtgaaaac caaagcatga cctcttcgaa aactgaaata tttatttcgt agaaaataaa
     1441 gttcttatac tttgtttcaa atatgagtcc tgcctttttt agttgagata gatgataaga
     1501 aattgtagca ttcgtcatat taaatttttc actaatttct cctgctgaca ttttattctc
     1561 cctcaacata accaatattt ctcttcttat agggtctgaa agagctttga atgtttctgg
     1621 aaaccccaac accatcacct cgctatttag aatttcatct aaatagaata taacataaca
     1681 tccattttat ttcaatagct atttagaaat ttttctaaat agttatttct aaatatttat
     1741 tgaaaatttt catattaaga ttctcaattt acttaagtcg cttttattta atcttacttt
     1801 atctaatttt tttaacttaa caaaagtgta ttgcaccata attgttaaag caaaaaccta
     1861 atgactgagg tacttatata tataacaata cttttaattt taaattaaaa gtagctaaac
     1921 ccaatttaaa aagtagttaa gtcctattta aatagaaaag aatgaaacga gaaatatact
     1981 gtataaaagt acataaaaac atctactaat tagatacaac tataacagaa tatatttatt
     2041 attataataa caaaagaata aaaaaaatta atttagcata accttgttaa atatagattt
     2101 gaatatgaat ttaaagttat gtaaaattaa aaatacagga atgaaaattc cattcctgta
     2161 taaaatctaa cttttgagtc tcagtacaat cttattttga gacaatcttt tttgttttat
     2221 attttcttat atttaaaata taacgcatat cttatgcaac atattcatat ttttctttat
     2281 attctttcat cgcatcattc caagcgtctt gtactgttac acctctttta ttagcaattt
     2341 tttgtgccag ttcaattatc ttcatagctc ttcttgatat gttcataaac attcctccta
     2401 caaattacca tatatttcat atatataaat caaggttata tttttatgat atataatcct
     2461 tctattgtaa gattacagtt tatatgtgta ttatatatga actatattag aagataatta
     2521 gaatttttat aaagctatat gattagatta aatataaaag ctttacaatt acttataaat
     2581 ttaaaaagtc tataagctaa actaatgttt gatataccaa ttttacgacc ataaataatg
     2641 ccattactaa aaatattggt tttatcatct ttgaaccttt ctttatagca actttagcac
     2701 ctacataagc tcccattatc atcactaatg caaacactat agcaatttta taatctactt
     2761 gaccatttat catgaataat aacaatgaag ttatattact tgttaagttt aatatttttg
     2821 tatttgcaga agcatgtaaa aaatcatatc catatatttt tataaatcca aaagtcaaaa
     2881 aagttcctgt acctggacca aaaaaaccat cataaaaacc cattataaga gccataagca
     2941 tccctagtct taaatttttc ttattaacag cttcaaaatt atcttcttga cctaaatttt
     3001 tagaaataaa ggtgtaaata gccactatta aaatcataac cacaaccaaa acatttaata
     3061 tctcttcact tattgataat acacatctca cacctagaac actaccaata atagtaaatg
     3121 gtactaattt ttttaaaagg ttattattaa tttttccaga ctttgcatat ctataggcac
     3181 tacttataca tcctgctgat gctgcaaatt tatttgtacc aatggcaaga tgtactggca
     3241 ctccaattgc cataagaaca ggcatgctta taagtcctcc tcctcctgct atagaatcta
     3301 caaaagcagc taaaaacccc cctatacata aaaaaataac tgaaaacata aaattcctcc
     3361 atcattctct ttcaattttt cttacaatat atattatata atcttattta atacttatta
     3421 tccagcatat tctttctata ttcatttgga gttatactat taaacttctt aaatatttgt
     3481 gaaaaataac tactgttatt aaaacctact ttcttacaaa tatcactaat actcatgctt
     3541 ctatctaaca gatatttttt agattcttct atcctaagaa gatttatata ctcagttatt
     3601 gaaactttat ttctttcatt aaaaatagaa ctcaaataat ttggacttat aaaaaaaaca
     3661 tctgatatac tattcaaatt tatattttct ttataatatt tttttatata ttcgtttata
     3721 ttttcaatta gatttccttt atcttttctc caaaatgctc tattcttatc aatacactct
     3781 ttggaaaatt caaataatac attccataaa tcttcatcac tgtttacaag tgatattttt
     3841 ttattatatt tatttcttaa ataagatata agttttaaat cctctaaatc tctaagcact
     3901 gttctagaca atacaaatat catttcctga atagtatctc taacatcatt atatcttaat
     3961 ttgtttgtat ctattagatt ataaacacct tctaaattca tatttttaat ttctttttca
     4021 atataaagtt ctaattcaaa gggatattct tttgaagaat tattgaacat atctatcgga
     4081 ttaataatat actcttcttc taataatcta ctatttgctt ttttaagtgc ctcttcaagt
     4141 cttatatttt cttttctact cttcaatatc tcctcatctt ttatactaag ttgattttca
     4201 aaaaaattcc tcttagctat ttcctgaata aattcagaca tcattaataa attttctttt
     4261 aatacatata atctactttt aggatttaat ggaaattcat tgtaaatttt gtttatgtat
     4321 tttatatcaa taccagaact acttgataat tcagataaat ttttatctat ataaatagac
     4381 ttatatacta taaaattacc acattctata tatccaacta tattttcttc taatactata
     4441 ggtatctcta aactggaaac accatactca caatgataca ctttttcaaa gttatcatta
     4501 tttatgcttt taacaagttt tttagtacac ggttttatat ttttacaaat atcgcaaaat
     4561 ttattttctt tcttagtctc aaatatttct ttatacttta agtcatatac agatattcta
     4621 agcttcaata tattagatat ttgatttgca agattttcaa ttatgtttac attaactaac
     4681 tcctgtatat tgttttcaga aaatactata ggtatttgtc tcaaactcga aaatttatga
     4741 ggaacataag taattaatat tactaaatca attttaccag tgtttataat ttcatgttct
     4801 gaatatggtg ggcaataaac tatagatgtt tcctttatat tgaccttttt accatcaatt
     4861 atttgtattc cttctccctg aatcacatat aaaagctgtt cttcacttaa atgaaaatgc
     4921 ttttcttggc agtttcccgg aaaaaatctt acaattccag cactaagtct attgaattgt
     4981 gtttttgatg gttcgtgtat ccacataacc tctccccaat caaatttttg tacttcatta
     5041 tttgactcaa taaaatccac cataaactca cctcgtagtt tttgtctaaa tattttttct
     5101 tcccttctta tattaaaata atatcatcat cattaacttt cattactata aaatcatcat
     5161 actcttttaa tactcctgta attttagttg cacttagttt actacaattc agttttttaa
     5221 tatattgata aattctataa ttactttttt attttgtaaa atcagtgttt tcccaaaata
     5281 attatatcta taaatgaaag tatttttact gcttacatca atatatgtta tcataatttt
     5341 cctttttatt gcatatataa ataactgtat ataacattgt ccaatactat atagaaatac
     5401 atctaagaaa gaatattatt aaattgttcc aattagtatt atttttctta tagaaataaa
     5461 aaaactgttg gattctttta gaaaaattat tttttacata ccttttttgc ttagatagtc
     5521 tatcttctca gctattttgt atatacactc ttctagttca tgattttatt ttaatatctt
     5581 gcttatattg ctgaaataat tgaattcttt tatctatcat attgtctctt atatcataaa
     5641 catattgttt cttatatcac aaaaatgaaa gcattcttat atcttcaata tataaacctg
     5701 ctttttttaa tcttgtaata aatataggat actctatatt ctcttcctag aaataactaa
     5761 taccacttat ttttctatta tatagaaagt aataaatctt tttcttaata aaattcaata
     5821 gtaagtggaa taaaatttta tatgaataag aacattataa aaatacttta tacaatagta
     5881 aataaaagat attagctcca aattatgtta aagttgtatt atttaaagtg aaaaaacaaa
     5941 taaaaaagaa ataatagtat aagtagaaaa atatacttaa aacaaggcga agcctctctt
     6001 gtatctaaat caagtagaga attaaatcta aaaaagaaaa taaatataaa agatgactcc
     6061 tgtacattac aagaatcatc ttatggtttc ataatcactc catggttttt aactgtctga
     6121 tataaaggtc tcagttcact catagcatga ccaccctttc cgattgctga ccccgtcact
     6181 tatggacaaa atgtccataa gtgatagtcc tctcttaaaa ccctctaaat agatatatct
     6241 caagcacctc attgaaacca gttgctggaa acaaactgaa gcgttctgga atagtaaaag
     6301 aaattgccac catatccacg ttactgtcct agttgttact ttgctaactt cggagcgatt
     6361 tcctctaaca atacgcagtc aaattctgcc tttttgctaa gaaatggcag atggtctgca
     6421 tctggtataa aaaaaatctg tttgtctggc gccactattt catcaaaaaa tttcttcgct
     6481 ggaagatagg gactattata gtcattttct cccatgatgt agtatacagg gacctgatag
     6541 tcaactccat aatttcttat gtcagcttct tccactaaaa accgcaatac atcaccttga
     6601 ttgtcaatcg catttggatt gacaatggcc gtatttttta actctctcaa tgaataatac
     6661 ggggttgcca gtatactgat tatattgccc aaatccatat ttatagaaag cccatacttg
     6721 ctttggtatg ttcttactgt cataagagct gccaaatagc ttggaccata ttttgctggt
     6781 ggatatggtg caatagcttc cagcgccttg atatcttttt gcttccctgc agctttcacc
     6841 atttcaagtg tcttttcata gcccacgcgc tcattgtcca ccatattaac ggtctgcgca
     6901 atcccaatgt acgcgctgta atcctccgga taggtttgca cagccatagt tcccagcact
     6961 gttccccagc tatgacctat aaggataatt ttctccttgt tgaactgttg cttcaaatat
     7021 gctgtcacct catggacgtc ttgcagaaca cgctccgcgc tcatgatctg caaaactgtt
     7081 gccggatcgt tcgcaaagta tgttttgcct acgtttcgct gatcccagtt caccacagta
     7141 aaatcatttt cccactcata ctggtacaga tgtaaaaatg gcatcattga attaccagga
     7201 cccccatgaa cccaaaggat gatggggttg tctgcgtttt gtccccggat atataacgcc
     7261 tgctgtatgc cgccgatctc caccatttcc atgcggtcga ttccaggagc ttctatttta
     7321 tggcttgtac gtataatcgt gggggctatc agacgcaaac cggcaaacgc cagaacaaaa
     7381 acagcaacta gtatgcaaag tgtgattaat atcattttaa gtaccttctt tcatgaattt
     7441 ttttagccat agatatctcc tctaacatta aaaaaagtat gttgctattt tagttttgtt
     7501 actctacctg tcccattggt ttcctttcat aaaaaatatt aattgattct ataatctcat
     7561 cttccaggat atcaactcct atttgtctca taagaaactg taagaattga aaccgtttac
     7621 gaattgtagg aaagtccccc tgaaatacat ctgtatcaca ccaaaagtta aagagcagca
     7681 aaattacctc tgcgcattca tctgggaact ctgtgatgat gcttctatcc tctatgcctt
     7741 ctcgtaaaac atttgctaat attggtgcta gttttttcaa attatcctgt gtaaatgcca
     7801 gtatgatatg cggactttct atcgcgcttg taatcatgtt actagattct tttatcacct
     7861 catccgttaa atgccgccta actaaacttc taagcttctc ttttgctgta agtccatgca
     7921 tttcaccgag ccattgattt actgtctcta ttatctgttc aaagcgcctt tccatgacca
     7981 cattaaaaat atcttctttt gaactgaagt gatgaaaaat tccgcccttt gacattcccg
     8041 aagcatcaac aatattttgc atacttgttt tgtcgtatcc tttttcagca aacaactttg
     8101 cagaaatggt aatgatattt tcaatagctt gctttggatt gttctttcta gtcattatat
     8161 aactccttct tattaaaaac cgactgttgg tttttattat ataagctatt cctgcttctg
     8221 tcaagcaaca ttatgtttat ggcaaaacaa taaggtttgg tggttagaca ggtattaatt
     8281 agactaatgc gactttcccc tagatggaac ccttggtaaa tttgcaatct ttaccggctt
     8341 tgattatatc tgtaagcaag gcaggtatgg ccacacctgc cctgtttgac cctcggatat
     8401 tcattcctat ccctcctctc cttagccatt acaccaaaac gccgctcatt gtctccaatg
     8461 gcggcgtttt aaagatatga tttctcatgc tgctgaaata tgcaaaaaat ataagatgcc
     8521 ctttattatt tttcctacaa tagaaaagaa aagtattgaa gaatgggtta atattggagc
     8581 aaatatgctt tgttttgcac aagatactac ctttatcact tatgttatca atcaattatt
     8641 atctttttat aatgagattg taccaattca aaaacaacat taaattataa atctaatttt
     8701 atattaaaag agactaatat tttatctatc tctattaagg atattttctt atgtatcata
     8761 atattagtct ctcttattat tatatactgc tatcatcatt agttgctatt tatactattt
     8821 ttataatata caaatttatt cagctaacgt tttgcctaat tctatacact catcttttgc
     8881 ttcttcattt gggtcttcca ttgctgtaat tccttctcca ccaataattg tagcaccagc
     8941 attttgcata cgttcttccc agtctctcat ccattcacaa tttccccatc catatgaacc
     9001 aaataatcca atctgtttac ctgataccat agattctaat tctgaaacaa atggctccat
     9061 atacccctct tctagttgct ctgctcccat agatgggcaa cctaatgcaa atttatcttc
     9121 ttcttttaaa tcacttggtt tcagtaagct cacatctaaa acttctggtg tcttaccttt
     9181 taacttcaca ccttcagcca caaaatttgc cattttttca gtatttcctg ttccactcca
     9241 ataaacaata tatattttac tcatttttac ctcctgtagt attttttaat taatcaatag
     9301 ttagttatag ctaactaaca tctaaaaaac aaaagatttc tttatctgaa atctctttat
     9361 ttttctaaat atagaatatc atagttgatt ttgaaattca atacttattg ataatcttta
     9421 tcatttatat tttatattca ttttataata tcaaaacact gtatcgtatt tctaatcttt
     9481 catagaaaaa tttctaactt atagtacttt tgcatctctt ataaatcttt tatatgtata
     9541 agtacataga aaaacacacc ttttccagat gtgctatcta taatatcgta tatttatttt
     9601 tcaattctaa ctagctaata tgtttataaa ttttatcact taataccttt atacattgat
     9661 gtaaaatctt acacctaagt aatttttatt atttgtctcg actctatata atatagctat
     9721 tttatcatca aatcaaaaat tatcttttgc cattattctt gtatttataa agatttaaat
     9781 ataagtatcc atttccttgt tccgttctag gtattccaag cgctctcgta cattttatta
     9841 attgtgcata cagttcagct tcatctaaat ctctttcaaa ctcctctctt gcccattctt
     9901 taattagtgc taatgagcct gatacgtatg gtgcactcat actggtacca ctcataatag
     9961 caagtttatt atccatataa gtcgatataa tatttcttcc cggagctacc aaatctattg
    10021 tagtatttga attactaaac ttttcaacta aatagttttc atttattgca cctacttcta
    10081 ttacctcagc ataactggct ggataactat actcacttgt actagaatca ccatctccat
    10141 tattacctgc tgcacacact acagaaatat tattttttac tgcttgcatg acagcatttt
    10201 ttaacttctt atcatctttg tttcccccaa gagacataga tataatatca accttgttat
    10261 ttacagcaaa gttaatagca ttaattatac tttgatatgt tccagtacca tctttattta
    10321 atgcttttgc tattaataat ttacaatctg gagctactcc cataacttca ttattatagt
    10381 tagatgcagc tataataccc gccacatgag ttccatgacc attaaaatcc tcatatatat
    10441 ttttatttcc attactgtca tcactaaaat ttgcaccacc aattattttt ccttttaaaa
    10501 gaggatgaga tatatcacaa cctgtatcta ttataccaac tacaatattt ttaccagtat
    10561 aaccttcatc ccacattccc ctagcattca tttgttttat tccatatggg aatttatttt
    10621 ttgcacctct tagattttca tttatttcat acggaattag tttcattctt ttattcatca
    10681 tatatcctcc taaaaatttt attgcttata aaaatcatga attttatttt ataatagg
//
LOCUS       DACXMZ010000133        10723 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.134, whole genome shotgun sequence.
ACCESSION   DACXMZ010000133 DACXMZ010000000
VERSION     DACXMZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.134"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            275..733
                     /locus_tag="JD016_12890"
     CDS             275..733
                     /locus_tag="JD016_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAU5000530.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            794..1069
                     /locus_tag="JD016_12895"
     CDS             794..1069
                     /locus_tag="JD016_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU5000531.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1128..2402
                     /locus_tag="JD016_12900"
     CDS             1128..2402
                     /locus_tag="JD016_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088840.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAU5000532.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPK
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2532..3584
                     /locus_tag="JD016_12905"
     CDS             2532..3584
                     /locus_tag="JD016_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAU5000533.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3584..4615
                     /locus_tag="JD016_12910"
     CDS             3584..4615
                     /locus_tag="JD016_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAU5000534.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKAIGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EENLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4707..5519
                     /locus_tag="JD016_12915"
     CDS             4707..5519
                     /locus_tag="JD016_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAU5000535.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5522..6442
                     /locus_tag="JD016_12920"
     CDS             5522..6442
                     /locus_tag="JD016_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HAU5000536.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6617..7063
                     /gene="rpiB"
                     /locus_tag="JD016_12925"
     CDS             6617..7063
                     /gene="rpiB"
                     /locus_tag="JD016_12925"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HAU5000537.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7093..7758
                     /locus_tag="JD016_12930"
     CDS             7093..7758
                     /locus_tag="JD016_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAU5000538.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTD
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7868..9370
                     /locus_tag="JD016_12935"
     CDS             7868..9370
                     /locus_tag="JD016_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAU5000539.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9682..>10723)
                     /locus_tag="JD016_12940"
     CDS             complement(9682..>10723)
                     /locus_tag="JD016_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAU5000540.1"
                     /translation="SMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFFMYIYYAEAIA
                     IVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVIGDDEISTLA
                     KSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDMLKNNDFDRET
                     INDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQSIVEFEDKIQ
                     NSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDMFIKEENIIL
                     TFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENAHMEIIIDAD
                     LFKVMLTFRRVN"
BASE COUNT         4276 a         1215 c         1966 g         3266 t
ORIGIN      
        1 tttgtaaaaa acaaaaaaat catttctcta acaaaaaaaa ccacaaaaaa tataaaaaag
       61 tatggctagt taagtgatta acaagtaaaa attaaaataa aaatcaaaaa aaatttatca
      121 atgaaagtta gctaaaatcc tggaagagag cattattttt gggaaaacat tttcttttgt
      181 ttcacaaaaa aagccacttt ttggcacaca acatgcttta tataaagtca agaaaaacta
      241 aggtataaga caaaggcaat taggaggtgt aaatatggag tttataaatt ttgtaagtga
      301 taaagttata gaccttaaaa tggattgtaa aacgaaagaa gatttcttta aagaaataca
      361 taataaagtt tttgatttag gctttgtaaa agaagaattt ggagaaaaaa tattagcaag
      421 agaaaatgta tttccaacag gtttaaattt aggtgattat ggagtagcaa taccacatac
      481 agatgcagaa tatattaaag agcaatttat atcagtctgt acttttaatg aaccagtagt
      541 atttagttct atggaagacc aagatgaaaa agtacctgta aatttggctt tcgtattagg
      601 acttaatcaa ccacacagtc aattatctgt attaacagaa ttaatgggaa ttatgcaaaa
      661 taaagagtta gttgataagt tgatgagttc tactgataaa gaagaagttt taaagacact
      721 aaagtcttta taaaaataaa aaagtactaa aaataaaaaa tataaaaata aaaattatta
      781 ggaggaaata aaaatgaaaa aaatattagt agcatgtgga gcaggaatag caacatcaac
      841 agtagtttgt gacaaggtag agagattagt taaggaaaat aatatacaag cagaagttat
      901 acaatgcaaa atagctgaat gtgcaacaaa gcaagaagga gcagatttaa tagtatcaac
      961 aactatacta ccaacaactt ataatatacc agcaataaaa gcaacagcat atataactgg
     1021 agtaaataca gcagcattag ataaaaaaat attagatgct ttaaaataat cctatcatag
     1081 atacattgaa ataatatttt ataaaacaga aaaaggagga tttataaatg gataaattat
     1141 tagcagtagt acaatacatt ttaaatatgg gaccgacagt aatactacca ataacaattg
     1201 tagtactagg aatgatattt agaattaagt ttacaaaagc tttaaagtca ggtcttacaa
     1261 ttggtatagg atttgtgggt ataaacttag ttgtaaacct attaacatca acactagggc
     1321 cagcggctca aggaatggtt gaaagattcg gacttaattt aacagtaata gatgttggtt
     1381 ggccaacaac agcatcagca acatgggctt caccagttgc accaatatta ataccagttt
     1441 gtttattagt aaacttacta ttattatact tcaataaaac aaagatatta aacgttgata
     1501 tatggaatta ttggcacttt atagtagcag gtgcagttgg atatatagta acaggaagtt
     1561 atgtttgggc tatattctgt gcaatactaa tggagttatt agtactgttt attgcagaaa
     1621 agacagcaaa aaatgttcag gagttttatg atttagaagg tatagcatta ccaactggtt
     1681 caacagcttc atttgctcca cttggatatt tagtaggtaa atttgttgaa aaaataccag
     1741 gaataaataa aatacaagca gacccagact ctatacaaaa aagatttggt atatttggag
     1801 aaccaatgat gatggggatt atactaggta taatacttgg agcacttgcg ggatatgatg
     1861 tagcaggaat attcaatgta ggtatatcaa tgggtgcagt aatgttactt atgcctagaa
     1921 tggttagaat attaatggaa ggtttaattc caatttcaga atcaataaga gatttcttac
     1981 aagcaaaata tggagacaga gaactttata taggtctgga tgcagcagta gcaactggac
     2041 atccagcagt tatatcaaca gctcttatac ttgttcctat aactttgttc ttagcagtta
     2101 tactaccagg aaacaaagtt ttaccatttg gagacttagc gacaatacca ttttatatgg
     2161 catttatagt gtgctttaga aaaggtaata tagttcaatc agttataaca ggaacaatag
     2221 taatagctat atcactttat atggctacaa actttgcaga tgttcataca caattattac
     2281 aacaagcaaa tatgatgcca aaaggaacaa caatggttac aagtatagat acaggtggaa
     2341 acttgctaaa atggggaata ttgaaattat ctcaattagt ttcttcattt ggaatattct
     2401 aatatgtagt taatagagat tactcttttt tagtaaagta tgcgaaaatt taacatttag
     2461 cagctactaa aaaagagtaa tattttttat aaatttttaa aaatacaaaa tacaagagga
     2521 ggagatttat cgtgaaagca gcagtattac atggcacaaa tgatatgaga tttgaagata
     2581 tagaaataaa accatgtgaa agtgatgaag taaaaataaa agttatggca gcaggaatat
     2641 gtgggtcaga tcctccaaga gtattaaaac attggaaata tccagttcca gctataccag
     2701 gacatgagtt ttctggagta attgctgaag ttggtaagga tgtaaaaaat gtaaaagttg
     2761 gagatagagt agttgcaatt ccttttatac cttgtaatga atgtgaatat tgtaaaagag
     2821 gattattttc attgtgtgat gaccatggaa tgttaggagc taaaagtttt ggagcttttg
     2881 cagagtatgt aaatataaaa gctacaaatg tacttccaat aggagatatg gactttgaag
     2941 atgctgcaat gatagagcca ctagcagttg ctatgcatgg agtacttaat ataggtgttc
     3001 aagttggaga cactgtagcc gtaatgggta gtggaacaat ggggcagttg gtaatacaag
     3061 gacttaaaat agcaggggca ggaacaatta tagcagttga tatatctgat aataaattaa
     3121 gagaatcaaa ggaattaggg gctgatataa taattaatgc taaagatatt aacccagtag
     3181 aaaaaataaa agaacttact ggtggaaaag gtgttgacat agctcttgaa tgtgcaggtt
     3241 caaaaataac tcaagaacaa tgtttactta taactaaaaa gaaaagtaag ataggatttt
     3301 tagggatagc atattcagat ataactttat cagaagaagc atttgaaaat atatttagaa
     3361 aagaactaga attaaaaggt ttttggaact cttattctgc accatttcca ggacaagaat
     3421 ggactaaggg aatcaatcta gttaatgaag gaaagataaa attaaaagaa atggtatctc
     3481 atagattctc acttgaagat acttacaaag catttgaaat gataagagat agaaaagaag
     3541 agtttaacaa aatattaatt ttaccacaag gggttgaaaa ataatgaaag cagtagttaa
     3601 aactaaacca ggctatgata atgtagaagt tttagaagta gaagaaccaa aagctatagg
     3661 agacaaggtt aaaataaaag tagaatatag tggtatatgt ggttcagata tacattcatt
     3721 taaaggtgaa tatgcaaata taaaagcccc agtaacatta ggtcatgaat tttctggaat
     3781 tgttgtagaa gtaggagaag atgttaaaaa tataaaagta ggagatagag ttacatcaga
     3841 gactactttt gcaacttgtg agaaatgtat atattgtgaa actaaagatt ataacttatg
     3901 cccaactaga aaaggtattg gtactcaagt aaatggaagt tttgctgagt atgtattatc
     3961 aagagaagaa agcatacatg tacttcctga aaatgtatct ttattatcag cagcccttac
     4021 agagccacta gcttgttgtg ttcatgcagc tcttgaaaag actactatag aaagttctga
     4081 tacagtttta ataataggac caggaccaat tggtttatta ttagctcaag ttgttaaatc
     4141 tcaaggtgct acagttataa tgtctggtgt tacaaaagat aaggaaagat tagaaatagc
     4201 taaaaatctt ggagtagata gaactgtaaa tgtaatggaa gaaaatttgg cagatgtagt
     4261 gaatgaaatg actgatggaa atggagtaaa caaaggattt gattgttctg ggtttatgcc
     4321 agcagttaac gaagccttaa gactgcttag aaaaaaaggt actttcgtac aagttggaat
     4381 ctttgctaaa aaattaaatg aaatggacca agaagcaata atccaaagag agttacaata
     4441 tataggaagt agatctcaaa agccaagttc ttggattaca gcattagatt taatggcaag
     4501 tgggaaagta aatacagaag ctttagttac tagaactgtt ggtttagacg aatttaaaga
     4561 aggtattaca gctttaatga acggggaaga aataaaagta gcagtaaaat cttaaaaaaa
     4621 catttgtatt attttaaaaa aaattgtaaa atctactgta ggataaaaaa atgtaaggtc
     4681 ttaaaaaata acaggaggaa gaaagtatga gcactcttag agatcattct aataatatta
     4741 gaaaaaacat aataaaaatg gttgcagaag ctaaaagcgg acatccaggt ggttcattat
     4801 caatagctga tatgttgaca gtattatatt ttgataagat gaacgtatgt gcagaaaatc
     4861 caaaaatggc tgatagagat agatttgttc tatcaaaagg tcatgcagca ccagctttat
     4921 atgctacatt agcagaaaaa ggatttttcc cagaagaaga attaataaca ctaagaaaat
     4981 ttggttctaa gttacaaggt cacccagaca tgaaaaaagt agcaggaatc gatatgtcta
     5041 ctggttctct tggtcaaggg ctatctgcag caaatggtat ggcacttgca ggaaaattag
     5101 ataacaaaga ctatacagta tatactatat taggtgatgg agaaatccaa gaaggtcaaa
     5161 tatgggaagc tgcaatgaca gcatcacatt acaaattaga taatttaatt gcatttgttg
     5221 acttaaatgg attacaaata gatggttcta atgaagaagt aatgaatgta aatccagtag
     5281 atgacaaatt caaatctttt ggatggaatg taatagtaat aaatggacat tcttttgaag
     5341 aaataggaaa tgcagttgat gaagctaaaa aagttacagg aaaaccaact gtaataatag
     5401 ctaaaacagt aaaaggtaaa ggtgtttcat ttatggaaaa taatgctgca tggcatggaa
     5461 cagctccaaa tgcagaacaa gcagagcaag cattgaaaga tttagaagga ggtctatagg
     5521 aatgggaata gcaactagag aagcttacgg acaagtgtta aaagaacttg cagaaaataa
     5581 agatatagta gtattagatg cagatttagg aaaagctaca aaaagtatat catttaaaga
     5641 agttgcccca gatagatttt ttgacatggg gatagcagaa ggtgacatga ttggtacagc
     5701 agcaggtctt gctacttgtg gaaaaatacc ttttgcgagt acttttgcaa tatttgcagc
     5761 aggaagaggc tatgagcaaa taagaaactc tgtagcatat ccaaatttaa atgtaaaaat
     5821 agcagctact catgcaggtg taacagtagg tgaagatggt ggtagccatc aagctataga
     5881 ggatatatct ctaatgagag gtataccaaa tatggttgta ttaaatccag cagatgcttt
     5941 agaagcaaga caagctattc ttgcatctat tgattataat ggacctgttt atataagatt
     6001 aggtagagct gcaactcctg atgtaaacag tgaaaattat aaatttgaaa taggaaaagg
     6061 aactgtttta agagaaggtt ctgacataac agtaatagct acaggaataa tggtagcaaa
     6121 agccttagaa gcagcagaag aacttgctaa agaaggtata aatgtagaag ttgttaatat
     6181 atctacaata aaaccattag atgaagcatt aataaaagaa actgctaaga aaactggaaa
     6241 agtagttaca gcagaggagc acagtataat aggtggttta ggttcagctg tatgtgaagc
     6301 tttagcagaa actaaagatg tagtagttag aagaattggt gttaaagatg tatttggtca
     6361 atcaggtaca ccagctgatt tattaaaaca ctatggttta actacagaag atatagtaaa
     6421 aaatataaaa gagttattat agatataaat atatctaaat aacaaattga tttgacataa
     6481 gtcaattaaa gctgtcttaa aaatagaaag aaatttctac aatgggacag ctttattttt
     6541 acaatacttt atttttatga taattataaa atagtctgaa agtaatcaaa aaaattataa
     6601 aaagaggtgc tatttaatga aaatatctat tggatgtgac catggtggtt atgaattaaa
     6661 ggaatatata aaggaaaatt taataaataa aggtatagaa gttgaagatg tgggtacaaa
     6721 ttcaaaagaa agtgtagatt ttccaatata tgcaaaaaag gtaagtcaaa atgttcaaag
     6781 taaaaaatct gatttaggca tactttgttg tggaacagga ataggtatgt ctattgcagc
     6841 aaataagttt aaaggaatca gagcagctgt tgtttctgac tgtttttcag ctaaggcaac
     6901 tagagaacac aataatacaa atgtactttg tttaggagaa agagttattg ggaaaggatt
     6961 agcaatgaga attgttgaag aatggttaaa ttcaagttat aatggagaaa gacatgcaag
     7021 aagattacaa atgattgatg atatagaaaa agaaaattta taaatatata atttataaca
     7081 agggggagcg ttatgtctat tatttgtgct tcaataatgt gtgcagacca aatgaagtta
     7141 aaagaggaat tagaagctct tgaagaagca ggtgtaaaat tacttcattg tgatgttatg
     7201 gatggaatat ttgttaaaaa cttggctatg ggaccagagt tattaaaatc aataagtgaa
     7261 aatacaacaa tacctttaga catacactta gccacagaaa ctccagataa atatattgat
     7321 atgatgtctt atataaagcc aaaatatatt tcttttcatg tagaatcaag tacaaatgta
     7381 aaagctgata tacaaaagtt aagaaattat ggtatagggc cagtgttagc aataagccct
     7441 caaacaagtg tggataaaat agaagaatat ataagtttag ttgagggtat cttagtaatg
     7501 acagtaaatc cagggtttgc aggtcaaaag tttaatcttt ctgtattgga taagttggat
     7561 aaacttacag atatactaaa agattatgat aatcctccat ttatagaggt tgatggaaat
     7621 ataaataagg atactattaa acttatgaat ggtaaaaaag tagatatata tgttgttggt
     7681 acttcagctt tatttaatga taagcctcca atttcatata aagataaaat agaagaacta
     7741 aaagaaagta taaagtaacc atgtaataaa attataatat gtttctgttt aatagtaaaa
     7801 atttaggtgg aaacaagaaa ttttataata tttaataaaa attttggttg agtaaggaga
     7861 attttttatg ggagacctat ataaactaca aaatggtact gatattagag gaatagcgta
     7921 tgaaaatgat tctaaagaag ttaacctaac tgtagaagaa gtaaaaaaaa tagctaaagc
     7981 gtttcatatt tggttaaaag aaaagactaa gaaagataaa gtaactgttg ccataggaac
     8041 tgattctagg ataacaggaa gccaatttag aagtactgtt attgaaactt taactaatga
     8101 cgattgtaat gtaattgatt gtgagattgc cacaactcca gctatgttta tgacaactat
     8161 tatggatgga tacaattctg atggggcgat tatgattaca gcaagtcact taccttatta
     8221 ctataatggt ttaaagtttt tcacagaaag tggaggttta gaaaaaactg atataaaaga
     8281 gatgttggat atagctgtta aaaatgatag tgtagactat gaggaagtaa ataaaaaagg
     8341 tgaagttata gtaaaaaatt taatagaaga ttattctaat ttattgatag ataaaattag
     8401 aaaaggtgta aattcaagta aaaattatga aaagcctttt tctgggctta agatattggt
     8461 tgatgcagga aatggggctg gtggattctt tgctgaaaaa gttttacaca tactgggagc
     8521 agatacgaca ggtagtcagt ttttgaaccc agatggtatg ttcccaaatc atatacctaa
     8581 tcccgaaaac aaagaagcaa tggaaagcat atgtaaagct gtgttggata ataagagcga
     8641 cctaggaata atctttgata cagatgtaga cagagctgct attgtaggta aaaatggcaa
     8701 accaataaat aaaaatgctc ttatagctgt tatatcatca atagtattag aagaacatcc
     8761 aaaaacagct atagtaacag attcgattac ttctgaagga cttgctaaat ttataaatga
     8821 attacaagga agacatcata ggtttaaaag aggatataaa aatgtaataa atgaagctat
     8881 aagattgaat agtgaaggtg aagagtgcca tttagctata gaaacgtctg gacatgctgc
     8941 tttaaaagaa aattatttct tagatgatgg ggcatattta atagctaaaa ttttaataaa
     9001 agtagcaaag ctatccttgg aaggaaagac tatagaagaa ttgatagaaa accttgaaga
     9061 agctcaagaa gagaaagaag ttagaatagg cattaataaa gaagatttta gaccatatgc
     9121 agaggatata ttaaaagatt tagattctca tgttaagagt attgatggat ggtctgtagc
     9181 accaaataat tttgaaggca taagagttaa ttgtgatgaa tcaaatggag atggatggtt
     9241 tttacttaga atatcactac atgaaccatt attagcttta aatattgaat caaataaaat
     9301 tggcggagca gaacaaattt attctaagtt aaaacttttc ttagaaaaat atgatttaaa
     9361 aggtctttag ggtaaataga aagaatatat ttatattgaa gaacaaatgt aaatttgtac
     9421 ctaagattac aaatattaca aaattgtaat cttaggtaac agagatgtaa ctgattatgg
     9481 aaacactatg tgatattata gatacagaga gatgaaatat atgatgaatt atagagaaat
     9541 ataatttaaa atgtattaat atcacaccaa aaaggtataa tcaaaaaata tgtaaatata
     9601 aaccccaaca tttagaaaaa gacctctaag gaggtttttt tcttgtttaa ataacttaaa
     9661 atatattttt atattaatat atcaattaac tcttctaaaa gtaagcatta ccttaaataa
     9721 atcagcatct ataattattt ccatgtgtgc attttctaat tcaattatat tttgagcaat
     9781 tgataaacca agaccagagc catctgtttt tctagatata tcaccccttc taaatctttc
     9841 tattaactct tctggcttta gatttagttt atcatttgaa atatttttaa acgttaatat
     9901 tatattttct tcctttataa acatatctat atagacccta gtgttaggca tagagtattt
     9961 tagtatattg caaattagat tctcaaaaac tctccaagtt tttttaccat ctatcatggt
    10021 aaaaacagca tattcaggtg tttctattat aaaatctaaa ctgctatttt gaattttgtc
    10081 ttcaaattct acaatacttt gaattaaaag ttctcttata tcggtttttg ttatattaaa
    10141 ttctacatct ccactactta gcttagatgc ttcaaatatg tcatctatta ataattttaa
    10201 tctttgtgat tttttatcta aaatatttat atagtcattt atagtctctc tgtcaaagtc
    10261 attatttttt aacatatcaa catagcttaa tatagatgta agtggagttt ttaagtcatg
    10321 agatacgttt gtgataagct ctgccttcat cctttcattt tttatattag catctacagc
    10381 agtttcaaga ccgcctgtta tattatttat acttttggct aaagtagata tttcatcatc
    10441 tcctataact tctaatttat actttaaatt gccattttct atatttttta tattttttga
    10501 tatgttattt atgtaattaa acttaacaga taaaaagttg tatatataaa aatgaaaaac
    10561 tgtgtttaca agtgtaaata aaattactat agctattgcc tcagcataat atatatacat
    10621 aaagaatatc aaaacaagat attggataat agcttgtaca actatagata ttaaaaatac
    10681 tatagctaat tttttattta tagttttttt attatacata cta
//
LOCUS       DACXMZ010000134        10668 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.135, whole genome shotgun sequence.
ACCESSION   DACXMZ010000134 DACXMZ010000000
VERSION     DACXMZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10668
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.135"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(507..1913)
                     /locus_tag="JD016_12945"
     CDS             complement(507..1913)
                     /locus_tag="JD016_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU5000541.1"
                     /translation="MDSVKYILRRFIGSSILIALLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLVLRDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKFINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKGQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(1958..2671)
                     /locus_tag="JD016_12950"
     CDS             complement(1958..2671)
                     /locus_tag="JD016_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000542.1"
                     /translation="MIMESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEE
                     AVRVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLG
                     ADDYIVKPFLPKELTLRLVGILNRVYVPIEEEALPVFKLGDSVVVNLNSACVEKDGQE
                     ISLTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTP
                     STPKHLITVRGLGYKLMIN"
     gene            2836..3750
                     /locus_tag="JD016_12955"
     CDS             2836..3750
                     /locus_tag="JD016_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000543.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNKGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3750..4511
                     /locus_tag="JD016_12960"
     CDS             3750..4511
                     /locus_tag="JD016_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter permease"
                     /protein_id="HAU5000544.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4523..5260
                     /locus_tag="JD016_12965"
     CDS             4523..5260
                     /locus_tag="JD016_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter permease"
                     /protein_id="HAU5000545.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
     gene            5727..5965
                     /locus_tag="JD016_12970"
                     /pseudo
     CDS             5727..5965
                     /locus_tag="JD016_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6138..6407
                     /locus_tag="JD016_12975"
     CDS             6138..6407
                     /locus_tag="JD016_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HAU5000546.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(6625..6870)
                     /locus_tag="JD016_12980"
     CDS             complement(6625..6870)
                     /locus_tag="JD016_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HAU5000547.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(7185..7565)
                     /locus_tag="JD016_12985"
     CDS             complement(7185..7565)
                     /locus_tag="JD016_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAU5000548.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYI"
     gene            complement(7766..8959)
                     /locus_tag="JD016_12990"
     CDS             complement(7766..8959)
                     /locus_tag="JD016_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAU5000549.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            complement(9022..10242)
                     /locus_tag="JD016_12995"
     CDS             complement(9022..10242)
                     /locus_tag="JD016_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel protein"
                     /protein_id="HAU5000550.1"
                     /translation="MIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLIDTIFGTVLLKVT
                     DIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGEEEIIPLRLVPFI
                     ISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLVTGMAAGFAGLFE
                     TPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASSVSKALGLEKFSFALSSKVVFDLS
                     IFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGVCLSVLFLLFYKG
                     RYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTPLFSIGASLGVLL
                     AGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYFFVVCAISYIFNM
                     DKSIYSLQKISTKQ"
BASE COUNT         3745 a         1704 c         1306 g         3913 t
ORIGIN      
        1 tctactttag tagaacttaa tggcgcaaaa tacttcaatt taaaacattt atcgtacttt
       61 aaataaataa ctataataat atacttacaa tagttacata gaagctatta taactacaca
      121 gaacagcgta taattaaaac ttttttagga tgtcattata ttttttctta aaattaaaaa
      181 acactatacc ataattctat tttagtagaa tttttttgtc aataataaaa ttaaatatta
      241 actaaaaaat aaaaaaaatg gaattatcta aagaatttaa taaccttaca ataattccaa
      301 aatattattt ctatgaaaat tttgatacca tgagtaactc agccataagc gcataaagca
      361 caagttactc tttactttaa taaaacttga caagaaaaga atttatcctt attctactct
      421 aatagagtta atttgtcaat aaagaataaa attattttca aaatattttc attcttcatt
      481 cttatctgtt attattttat aaaatatcac ttaattagag gtaattttag tattgttttt
      541 agtccctgac tatcaatgct cgtaatatct aatttacctc tatgtgcatt aataatgcca
      601 gatactatca aaatacctag accatgattt tgacctttgg catatgcctc acttagtcca
      661 ttttggccct ttaataaact attaattctt tcttgactca taccttttcc atcatcttca
      721 acaattatat agcaaaaatc taaatcagac ttagaggtaa cccttatatt acatccttgt
      781 tggttatgag ttatactatt ttgtattaaa ttactaatag ccctttttaa taatttttta
      841 tctgcatcta taataatatc ttcatttagt atatcaagtt caacgtaaaa gtcattatcc
      901 aaattattat ttataaactc agaaataagc tctctcaaaa acgcagaaac tctaatctta
      961 tcacaattca atgcttgcat attatattca agtttagaca ctaaatttag gtcatttact
     1021 aattctctga ttttaatccc ttgattgcat ataatagaca cttgctcttg ttctctattt
     1081 gaaaggctac tgctttcttc aagttctcca gcatatccaa ttatcattga aagtggagtt
     1141 cttatatcat gagatattcc tgcaatccag ttagacctag cttcctcttt cttcctaagt
     1201 ttttcatctt tcatttgcaa ctctttagat acagtattaa taaattttga aatatcactt
     1261 agtattcctt tttcttgtaa aagtactggt tctccttttg acataagctt tatgccagta
     1321 ataattggtt ttatagattt caccattttt aaacctatga atagagctat taaaaaagta
     1381 actattgcat taaccaaaaa catacctatt attaacatag gaaattgttc tatttcctta
     1441 accgtataag taaggttaaa cttagtgtac ttgtcttttg gataacctgt tactaatatt
     1501 ccatttggat gttcccatac atacacagga tagtccttta aaaagaacct tgaaaacttt
     1561 gctacatctg tcaaagtata atgacttgga atttcttttg gtaaatcaaa tccccatatt
     1621 ctatctccat tgttatcaat taacattgcc caaactttat ttttatgtag tacttcctta
     1681 ctagaatcat ctaaaataaa ctttccatct cttaaaacca aattatcttc aatcataatc
     1741 aagtttgatt ttatttctct gctatcactt gtatctaaat tatcagagta tttagataca
     1801 aataacatat atgaaaacag aagaaaacct gctatattta atacaataaa aagtaatgca
     1861 atcaagatac ttgaacctat aaatcgcctc aatatgtact ttacactatc caaaattaaa
     1921 tcactcctct tgatttcttt tattctaata tctaaaatta attaatcatc aacttatatc
     1981 caagacctct tactgtaata agatgtttag gtgtagatgg agttttttct atcttctctc
     2041 taacccttct aacatgaacc ataagggtat tttcatatcc atacatatca tctccccaaa
     2101 tggcttgaca tagtgaatca ttagtaacaa ttctcccttt attttcatac aactttaaaa
     2161 gcaacgaatg ctctttagct gttaaagaaa tctcttgccc atctttctca acacatgcac
     2221 tatttaaatt cacaacaaca gaatctccca gtttaaatac tggcaacgcc tcttcttcta
     2281 taggcacata tacacgattt aatataccca caagccttaa agtaagttcc tttggtaaaa
     2341 atggctttac tatataatcg tcagccccta agcccaaacc aatcaatcta tcttcatctt
     2401 cacctcttgc agagagaaaa atcactggta ttttagatgt ctctctaatt tttctcataa
     2461 tagaaaaacc atctccatct ggcaatacaa catctaaaat tgcacaatct ggctttacat
     2521 ttctaaatac cctaacagct tcctcataac ttgatgctaa aaatactcta ctaaatccct
     2581 cttttctcag gatttcatct atcattttta ataattcctt gtggtcatca accacaagta
     2641 tttttttatc ttttattgac tccataatca tcaccctcct ttagtatacc atatacccat
     2701 tttcacaact ttgcctaaat agacttttaa ggttattgta aggtaaattt cagctaattg
     2761 aaatgtaacc aagttattat actgatataa gttaaaagaa tcaataagca cagattaaaa
     2821 aaggggatga aacatatgga aatgataatt agaacacaaa gactttcaaa ggaatacaat
     2881 aaaatattta gagtaaagga tatagaccta agagttccac aaggagctgt atatggtttt
     2941 ttgggtccta atggagctgg aaaatctacc actcttaaaa tgcttcttgg acttgctaaa
     3001 cccacacaag gaaatattaa tattttaggt aaagagttaa atgaaaaaaa tagaatatca
     3061 attttaaaag atataggttc tttgatagaa tctccatcat actatggtca tcttacaggg
     3121 cttgaaaaca tgaccataat gcaaagactc ttagatttac ctaaaaaaaa tgtaaatgaa
     3181 gcattaaaaa ttgtaaggtt agaaaatcaa aaaaataaaa aagttagtca atattcttta
     3241 ggtatgaagc aacgtcttgg cattgctatg gcaataatga agtttcccaa attattaata
     3301 cttgatgaac ctacaaatgg actcgaccca gcagggattg aggaaattag agaattaata
     3361 aaatctctac cagaaaaata cgggatgact gttctaatct ctagccatct tttaagtgaa
     3421 attgaccaaa ttgccacatc aataggaata ataaatcatg gcgaactggt ttttcaagat
     3481 agcataaaag aacttcataa taaaggtcaa agtcagattg ctatcaaaac taagaatgtt
     3541 gtatctgcac aaaaactatt aaatactaaa ggatatatgt cacttataca agatgggtac
     3601 ttgactattg aaaatttaaa agactcagat gtggctaaaa taaacggtat gcttgtccat
     3661 tcaaatatag atgtatttag aatagaagaa cataaaaaaa gcttggaaga tatattctta
     3721 gaacttactg gtaaggcggt gactttataa tgaaatacct tggtctagaa ttttataaac
     3781 ttaaaagaaa gaagattatt ctgatgacct ttttatttgt agctgttgaa ataatgtggt
     3841 gtatcataaa ttcaaataga gctattacac gtaatccaga tatgttaggt ggatttgaat
     3901 attcctatat gcttatgagt tttgcaagtt taaatggtct attctttcct atcttaatat
     3961 caataattac atcacgtata agtgatatag aacataaagg agatacatgg aagcttttaa
     4021 aatcttctgt gacatcttta aatagtattt atttatctaa atttttatgt tctgctattt
     4081 tggtatctat acctgtattg atgcaagtgt tatctattgt gttgtttgga cattttagag
     4141 gggttattga gtcaatttct gtatctttac ttttaaaata cacattagga acaattcttg
     4201 taagtattgc tatcattgca ctacacctat ggattgcaac tgtaattcca aatcaaatga
     4261 ttgcaattac atttggcatg attggtagtt ttataggttt gacatcaggt ttgttttatc
     4321 aaggtattcg tagattgttt atatggtctt attacctaga gttatcacct ttaagttatg
     4381 cttatgataa tgttttaggt tcatctgtct atagaattga tatgaatttt tctattacga
     4441 ttttagtttt cttaattgga atgctaattt attatattgg aagaaatcat ttattaaaaa
     4501 aagaagtata ggagggattt aaatgttaag gaagtcagtt ttgggagaaa ttctaaagtt
     4561 aaaaaattct tttatatggt atgtattact agctttgcca cttataagtg tgttgattgg
     4621 ttctggtaat ttttatctaa atcaaggcat actcaaaaaa gaatggtaca gcttatggac
     4681 tcaagttagt cttttttatg gggaattttt ctttcctatc ttaatcgcaa tcttttgtgc
     4741 atatatttgt aggcttgaac atatgaatca taattggaat aatgtaatga cactgccagt
     4801 taatttaaaa aatatctttt tatctaaact tacagtagtt tcaatactta cagctattac
     4861 acaagttttc tttgttatct tttatatagt tgcaggaaat atgtttggtt tttctggtag
     4921 tgtacctaaa gaattgactg gttggattgc taaaggttgg attatttcta tttcaatagc
     4981 ttctatacaa ttatacatgt ctataaagat tagaagtttt gcaactccta ttggtattag
     5041 tctttgcctt tctctatttg gtatgggatt aatagtagca aaacctgctg ttggtatatt
     5101 tttcccttat tctatgttag gtagtgctat gggggttata aagcaatctg ctttaaatgt
     5161 taatgacact atagttacaa tatgtgttgc atttattact actatagtat tttctgaaat
     5221 tgccaataga aattttagaa agaatgatgt tatttcttaa tggtaaatgc tagatatttt
     5281 tgggtttgaa atttatattc tttttagttg aaatctatat ccattttatt taaaaatata
     5341 tatcttttat ttgaatttta tatgttttat ctactcgcta tataatagat atactcctaa
     5401 aattaaaaaa tatataataa tgttattatt taaaattatt atgtattata ttaaaactta
     5461 atgcaaaaac aatttttata ttatataatg aaacttaatt aaatataggt aaaaaatcat
     5521 tcaatatatt aaataaatat ttaactttaa taatctatat ttaattatag ctataacata
     5581 gaattatcaa atatattgaa tgaatatttg actcttggaa tgtgtaatta aattaagttt
     5641 ggttattcga tggaaggcaa atttgatata aaactccatt taaagtgcca gataaaaact
     5701 ttatggttat ataaaagtta aaagtaattt atagtgttat agaacattca aagggattta
     5761 aagtaatcta tggtgttttg aaatattcaa agggatttaa actaatctat agtcttgtag
     5821 aatattcaaa gagatttaaa acaatctata atgttgtaaa atatattcaa agggatttaa
     5881 actaatctat agtcttgtag aatattcaaa gagatttaaa gcaatctata atgctgtaaa
     5941 atatattcaa ggggatttga agtaatttat tgtgttttga aatattcaaa aagatttttg
     6001 gaatagtttt tataaattta ccagtactaa taactttatg tttaacattt agatgaccta
     6061 aaatcaacta tttgcacttt tgtattcatc taaaatcatt ttataatatt tttaaataat
     6121 ggaaaggatg tttgaacatg tttgaatcaa gatgtggtgt ctgttgtgac agttgtacaa
     6181 aaaaagaaca agtaaattgc actggttgcc caactatgga aaaaccattt tggggtggag
     6241 aatgtaaggt taaaacttgc tgtgaaagta aggaacttaa tcattgtggt gaatgtgata
     6301 ctttcccttg tgatatgctt ttaaataagg gaaaagacca aggttttgac cctatggtaa
     6361 atattgagca atgtagaaaa tggcttgaag aaacagtttc taattaattg ttttaaaatg
     6421 ctttgttaag taatttattt aatcctttgt taggtagtat gtttagtcat ttgttgattg
     6481 gtatatttat acatttattg atgggtatat ttagccatac aaatagattt acataaaatt
     6541 tacgactaaa aaagagttga gtattagttt ttattattta aaacggatat tcaactcttt
     6601 tttatgtcta acttttttga ttttttaatc tttccaatac caccacttta atttttctgg
     6661 ttctggattt tcagttgatg caaaatatac tgcacatctg tatacatata actgacatct
     6721 gtctatgtgc tgtccacgca ttaaacattc cttttcatac atttgtgctg ggtttgcatc
     6781 tttaagtgat ttaatagttg tatatcctaa catgattaaa gatttttctg ttgctttccc
     6841 tacccctgga attttttgta gttctcccat acttcacctt ctttctggta tattcttttt
     6901 gtattatact tagtcccaga tattttttta ttatagttaa tatttaattt tttattatat
     6961 catatcaatt tatttatgct ttttcatata tgtatatttt attactacct ctaaagtact
     7021 gagtcactta attacatcat aatacagttt tatacaaata aaataatata tttatttttt
     7081 attttgctaa tagcaaatta ttttaaatta aatctacaca tttaaaaaga gttttataat
     7141 tgtagataaa ctattacaaa actcttttta gacaacaaat catattatat ataagcttct
     7201 tcccaattta ctacccagtt ttctaacgac tttatctttt cctctgttag ctcctcttcg
     7261 tgaaatgata ctaataaatt ccttataaaa ttattcttca aaagattttg cacatttctt
     7321 gatataaact ctaaatatgc cttttctttt attgcaactg tataatacat acacttgcct
     7381 gtttcttgaa catataagaa tcttttcttt gatagttttg ataaagtttt taaagctact
     7441 ttttctggta aattatgttc ttctttcata taatcaataa tctcatatga attggttttt
     7501 atatctaaat tccaaataaa tttcataata actaactcta attttgataa tttttttaaa
     7561 cacatagtta tcactccgct tctttatcta tataattaaa tattacaata ataatttcac
     7621 aaaatcaatt ctaataatca taacatacct taaaatctgt tcttaacttc taccttctta
     7681 atctcccaaa tgattttttt atattgtatt caatagtata atccttttcc aaaatataac
     7741 ttaccagtta aaataatctt tatttttact tactctcgcc ttttacagaa gttaataatt
     7801 ttttataagt tacatcaatt ccaaaagctc caagaggtgc agttataagt atagcgagaa
     7861 ctgctaacgt caaaataagt tgtccagatg ccatccccat tgcaagtggt acacctccta
     7921 ttgccgcctg tactgtagcc tttggaatat agccaatcat acagaacaat ctttccttta
     7981 ttgtcaaatt tgtcttaata aggcagaaga atactcctac catacggaat aaaagaactc
     8041 caaatattaa aattacagct ccaattcctg ctttaaatgc ataacttata tctactgttg
     8101 cccctaccaa aacaaacaag agaatttcag caccaaccca tagtttcgaa tactttatag
     8161 acaatcttct tgcaactaca cgatatttta tctgaaggaa aatcccaata ctcataactg
     8221 ctaataatcc agaaaatggc acaataccct ctaaagcagt ctctaaactt atcattaaaa
     8281 atgagatact taaaagcaac acccctttag cagaatctct catatgaacc ttcttaaaaa
     8341 atactgctaa acagattcca atcactgctc caactattac acctaatata attgatactg
     8401 gaatttgtaa aaaactaata gctgatatac tatttccttg tgccaatcct gtgaatactg
     8461 taaacatcac aattacgaaa atatcgtcta tagaagttcc tgctaaaagc atctgtggaa
     8521 tacttttttc tgtaccataa ccttcttcca ttaatttaag cattttaggt acaataattg
     8581 cgggagaaac tgctcctact actgcaccca tgactgctgc ttccaatata gaaacgccta
     8641 gtaattttgg tgctaaaact atcattccaa taatttcaaa agtagctgga ataaagcaca
     8701 taagtacagc aggtcttccc actttcttca aatcgtttat atctaaagat aatccagccc
     8761 ttgttaatat aataatcaat gcaatctttc taaggtcaga agaaatatct agtattgaat
     8821 tatcaatcaa atttaaaaca tatggaccta atataattcc tgtaagtaac attcccaata
     8881 agttaggtaa ctttatcctc ttaaaaatac ctcccgaggc cattcctaat aaaaaaatca
     8941 aagctaaact tgtcaacata ttcctaccct atcctttctc taatattctt tctcttctaa
     9001 tcatacatta tgttgtttta tttattgttt tgttgaaatt ttttgtagtg aataaataga
     9061 tttatccata ttaaatatat atgaaatagc gcatactaca aagaaataag gtaaatagct
     9121 gtatccaaaa acttctgccc ctataaatac aggtgcaaaa aaagtatttg tagcactacc
     9181 aaagacacta gcatagccaa gcgctgcaac taattcaatt ggcaaattaa aaaatcctgc
     9241 taacaataca ccaagactag ctccaatgga aaacaaaggt gtaacctctc ctccttgaaa
     9301 acctgctgac aaagtcagta ttgtaagtat aaactttaac agccagtcaa atgaataaat
     9361 ttctccacca tagaaactat tttgaattaa atttgtccct aaacctgaat atcttccttt
     9421 ataaaataaa aggaataata cactaagaca tactccaata atagcaattc ttatcatagg
     9481 attttttaat ctattcccta tttttctttt tgacaatttc agacaccatg caaatgctcc
     9541 accaaccatt ccaaaaataa ttcctaaaac aataagcttc caaaatattg acaagtcaaa
     9601 tacaacttta gaagaaagtg caaaagaaaa tttctctaat cctaaagctt ttgatactga
     9661 acttgctgta aaagaagcag taaacgcagg aaaaagcgat tgatactcca gtgaaccagc
     9721 taccaaaacc tccatagcaa atagtattgc tgctattggt gtctcaaata atcctgcaaa
     9781 accagctgcc ataccagtta ctagaaaaat actagaagca ttttttatag gcaacctttt
     9841 acctacccaa tgtgaaaaag ttgcaccaat ctgaacagca actccttctc gtcctgcact
     9901 accaccaaat aaatgggtta accatgtacc tgatattata aatggaacca atcttaaagg
     9961 aataatttct tcttctccat gccctacctc aaatattaga ttcattcctt tactactttt
    10021 tcctccaaat ttaaaatagc aataagcaat aacaactcct actactgcta aaaatggtat
    10081 cagatacatt ggatatgttt ctcgaatatc tgtaactttt agaagaaccg ttccaaatat
    10141 ggtgtctata agacctataa ttgcaccaac tggtatacct atgagtccta aaaaaaatag
    10201 tccaccatat gttttataaa cgcttttaat tcttattatc attttaatcc atattaaagt
    10261 tttaaatttt tctatcattt taattccttt ctatttgcat cataagattt tttgaaagtt
    10321 ttgttgcatc aactgtagtc aatgtaatga ctatcataat tgttttttaa attgatagga
    10381 tataggtata ctcttagccc attcttaata aaataattaa catataatct actatttttt
    10441 ctattaatta aatatttctt caaatttaaa aaataaaaat ttagtaagta attcacctcc
    10501 attttatcga tttaaacaac aaaaaaagct ggtggcatct atgatatcat agaagtcatc
    10561 agctcattgg cagtttagga tattatcatc caagggagaa cctcattccc ataattatta
    10621 tataattttt ttatttttca tatgtctatt ttatgtaaat tgtatttt
//
LOCUS       DACXMZ010000135        10628 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.136, whole genome shotgun sequence.
ACCESSION   DACXMZ010000135 DACXMZ010000000
VERSION     DACXMZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.136"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(61..693)
                     /locus_tag="JD016_13000"
     CDS             complement(61..693)
                     /locus_tag="JD016_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HAU5000551.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(765..1118)
                     /locus_tag="JD016_13005"
     CDS             complement(765..1118)
                     /locus_tag="JD016_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAU5000552.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKSQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1196..1399)
                     /locus_tag="JD016_13010"
     CDS             complement(1196..1399)
                     /locus_tag="JD016_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAU5000553.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            complement(1789..2019)
                     /locus_tag="JD016_13015"
     CDS             complement(1789..2019)
                     /locus_tag="JD016_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU5000554.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(2021..2540)
                     /locus_tag="JD016_13020"
                     /pseudo
     CDS             complement(2021..2540)
                     /locus_tag="JD016_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088737.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(2788..3237)
                     /locus_tag="JD016_13025"
     CDS             complement(2788..3237)
                     /locus_tag="JD016_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU5000555.1"
                     /translation="MELIEWNYTYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFI
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRESSKVLEKCGFKIEEYCHNS"
     gene            complement(3251..4177)
                     /locus_tag="JD016_13030"
     CDS             complement(3251..4177)
                     /locus_tag="JD016_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000556.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4302..4706)
                     /locus_tag="JD016_13035"
                     /pseudo
     CDS             complement(4302..4706)
                     /locus_tag="JD016_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
     gene            complement(4856..5476)
                     /locus_tag="JD016_13040"
     CDS             complement(4856..5476)
                     /locus_tag="JD016_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAU5000557.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5564..6262)
                     /locus_tag="JD016_13045"
     CDS             complement(5564..6262)
                     /locus_tag="JD016_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000558.1"
                     /translation="MYKLSYVVRVSTKDLDELKRRCYEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLNTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAKRGKW
                     KETLPEIAHEINIVNLTSDDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            6363..7178
                     /locus_tag="JD016_13050"
     CDS             6363..7178
                     /locus_tag="JD016_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HAU5000559.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHKDKLPKIT
                     PHTFRHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSARAEMERL"
     gene            7254..7436
                     /locus_tag="JD016_13055"
     CDS             7254..7436
                     /locus_tag="JD016_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000560.1"
                     /translation="MQSIFQKENADTPCRYRIYRHLPTYEKIIKFNISFSNKKIPNSF
                     SRIYITLLKLGWGINN"
     gene            7850..8617
                     /locus_tag="JD016_13060"
     CDS             7850..8617
                     /locus_tag="JD016_13060"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAU5000561.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEVKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(8945..9622)
                     /locus_tag="JD016_13065"
     CDS             complement(8945..9622)
                     /locus_tag="JD016_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000562.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(9772..10089)
                     /locus_tag="JD016_13070"
     CDS             complement(9772..10089)
                     /locus_tag="JD016_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5000563.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10111..10449)
                     /locus_tag="JD016_13075"
     CDS             complement(10111..10449)
                     /locus_tag="JD016_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5000564.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3537 a         1661 c         1511 g         3919 t
ORIGIN      
        1 gaaaaagctc ttataaaatg attattaaag taaagtttaa tataaataaa ttataaaata
       61 ttaaactaat ttatcaatat ttcctgattg aatatgctta atgtttgatt gaattttttt
      121 atatggccaa tcccaccatt taatttctaa taattttgaa attgtatcat tattaaatct
      181 cttttttata atttttgcag gtactcctcc aacaatagaa tacggtggaa cgtcattagt
      241 aactactgct cttgttccaa taattgcacc atcaccaata tttacacctg acataattat
      301 agaatcataa cctatccaca catcatttcc aattataatg ttacccttat tgtcccaagc
      361 gtccgttata tggttgatat cgagtttcca ttcttcataa aagatgggaa aagtatatgt
      421 ggattttgat ttcattgagt gatttccact agtcattaga aattttgcat tacatgctat
      481 tgagcaaaac ttaccaatta ttaatttatc attgtttata gggtaatgat acaatacatt
      541 gtttttctca aaatctcttg ggtcattata aaagtcatta tatattgtgt aatctccaac
      601 ttctatattt tcccttgtta ttacattttt aagatataca gtttgataat cattacttct
      661 tggatatacc ttatttgaac ttggaatagt catatttaaa tctctccttt aatatatatt
      721 atgtttttga ctattccact actacaatac atctcttcac agaatcactc cttgttaaga
      781 atcttcataa gataatggcg tcctttttta gtgttaagtc ggatattctt tcttagtaca
      841 ttctttttag cgtataactg acaaactgtc attttcttac caatagtgtc aatgctatca
      901 tcaaggtcat tttgtattgc tttttcctta tcccttaatg atatatcatc acgttttccc
      961 tttgggggtt tatcagtagg tacaagtttc caagagtaaa caaattgtga tttaccaaat
     1021 gaatatacat acttatatgc gtatcttcca tcttttctct ggctctctcc agttcttaaa
     1081 attctatctt tattatctcg tcttttttca gttgacatga taagaagctc ctctcaataa
     1141 ccgaattgag cttgatactt catatgaata ttataacata taaggctcaa tttcattaga
     1201 taaagtcaag tgtatcaacg attttctcaa attgttttct ctttatttga atgcgattgc
     1261 tattcataat gacccagcca gaatcaaggt tttcatcagc aagtttacgt aattttttct
     1321 ctccaatacg aaagtatttt gacgcttctt caatcgtcaa ggtatatttt tcccaaatag
     1381 gtacatcagt attgttcata gaatgtatca ctcctttcta tgttcgtagt tcagaagtag
     1441 atagatggct ttggtaagct ccattggtat ttcaaccaat cccattctta tgggcagaac
     1501 cgtgtcatac aggtgtatca ttatactgtg gtacaggtta cggcaaaaca accgttacaa
     1561 aggttattca caaatcacta tatagtagct ctcttgatta aggtcatggc gtatagtatt
     1621 tgttggctat ctgtatcaca aacgtatcta tctactttat tcagttgtca aagaacagtc
     1681 ggcattgatt tgcctataaa aaatcacaat agcttatggg cttttataga caaataatta
     1741 gtgattctcc atgtaccaaa tagcttgata catggagaga gggcgagatt aagacaaatt
     1801 aaatctcaaa atagcagtga ttagttttgt tcctagcatt cgctttgttt cttcttctac
     1861 atgaatatag ggatttccgt attcatcaaa taaagttttc tgtgaaagac gaatgatata
     1921 gtgttcaaaa tgtttaagta ttacgtttat tgcatttaca tcgccatgta cagcaagtaa
     1981 aataattgta aaaggtaata cttctttagt aggtttcatt tcaatgttcc tgcatgagtt
     2041 ctttcaactc ttttagtgca tttgttttgt gataatgtat ggtattttct gctaaatcca
     2101 tcaatgttgc tatgtcagca tttgtcatgt tgagaaagaa tgaaagtaga attatatctt
     2161 gttttctttt tgtaagtttt ccgattgctt gcgccattaa tacatcttca attcgtatgt
     2221 tgtagtcgta agaaaccata cagatgtagt tagaatcata atcatctaaa atactcaatt
     2281 tacttaattc tgcaagtgac aaagattcga gagataccaa ataatcattt ctgcgtttat
     2341 tttcgtcata aatatctcta gcatagttac gcaaaactgt tttacagaaa ctgtcgaatt
     2401 gatgttcaat tgtcttttca tcaaccataa gatttcaccc tctttccatc gcaaggagtt
     2461 aaataacctt gcttactcct atcacggtgg gagggcgtta aatcttcgat aaatttcaaa
     2521 aaaaagagaa aaaaatacac actctgccta tagggcaaaa atgtgtattc tttaaaacgt
     2581 atttaattta tattggttgt aagccaccac catattgatg tatggaaata tgcattggct
     2641 agcttttcat aaaaatacaa agatgcataa gaaattaata gaggggaaaa acttattctg
     2701 ttattaaaaa gtaggttaat tctaataaat gctattgcgg tcgaattaaa ctatatacat
     2761 ggcaatcaat catgtttcta ttctttatta tgaattgtga cagtattctt caatcttaaa
     2821 accacatttt tccaatactt tacttgactc tctattgtag gaatatacat tagcatgaat
     2881 tttgttaagg ttgtattttt caaaggcaat tttgcatatt tcttttactg catttgttgc
     2941 tatccctttg ccccaatagt tcctagcaag ccagtaacct aattcagcat ttttattaat
     3001 agaggtatct tcttcaaatg ttaggctgat acttccaata gccctttctt gataaaaaat
     3061 agcatagcta agttgtttaa catctggagt tgccaaacaa aaactaataa aatctctagc
     3121 gttttgctcc ctataagggt gggggaagtt gtcacgaaga ttctttgcaa tttctttatc
     3181 attcgcaaac tcaactaaat cggtaatata agtataattc cactctataa gttccatttt
     3241 tcttctcctt ttatgaaaca gctacaagaa atctttctaa gccaatacca cttatcaaac
     3301 agcgttcttt tttatttcca agcatttgat aagtccagtc aacaaatcca ccgtcaacta
     3361 tttcaacggt ttcatttcct tgtttgaccg ataatttgaa attgatacct ttataataat
     3421 tattatcaac atcatcatta tgaattgata actctatatt aggaaaaata agtttgatag
     3481 tttcaaccat tctgtcgaaa aatccgtcat tatctttgta tccgtttctt ttgcgtagaa
     3541 taatagagaa acgaacattt tctatttcac ttaatatatc tttataatag tttaagtgct
     3601 tttctaacaa cattttttca caagtgtaag accctgtatc tataccagat gaaaccatac
     3661 aatataagcc aaaatgtgaa tataaacctt tacctacaaa attttgtgct cttattaccc
     3721 tagcagtagt agccatgtgg ataggtatgg tattattttc ttcttttctt tttaatttat
     3781 cagcaataat tattgccaac atatttgagg ggtctgcaag tgtttctgtc cctcgtagag
     3841 cacttactac attatattga tctacggagc caaaaacgct acagctacct aatggagcag
     3901 agggagaaag cataatcgtc ttgatatcca tattgaatgc ttttctcaat aattttgact
     3961 ccaaagcatg aatttcttct ggatctatac cgctagggat agtaaatcta tttagttggt
     4021 atgactttag aatatcagtt gcagttaaag tatttgcttg tctatcaaac acttctaata
     4081 gaagtgagtt caaatctgat tttgatagag ctgataactt atcaactata ttcttatccc
     4141 caattttatt tgtaattcta cttaaaatat cactcatagt tatcctccat actgttactt
     4201 tcaaaataat tatagaatgg ttacaacaac taatctaggt catttgacca aaaatgaaaa
     4261 aacactgaat atttactatt caatattttt ttactttggg tttatttgat taaatcttga
     4321 aaatactgta atgcaaccct ttcagcttca acctttcgga caataggact ttgtgataat
     4381 tcctctctta actcaagata caggttgata gtagcctgca atgcattaac acagctttca
     4441 tattcatggt tagcaaccgt cttttctaat gcttcaatat attggggtgc gtccaattca
     4501 atttttctaa ctccacgagg taatttacct aagagaagct gtattaacga ggcaagtact
     4561 gattgtctta aaaaagataa aaaatcaatt gtttcaaata gttctctacg tccaatctta
     4621 gtgcaagcgt aatgaaccca aatccaaaat ctatcttcaa tccactgtaa gtctggagtt
     4681 gggaagtatg cttctttttt agtataacac ctctcacttt cttttattag ctatcaatcc
     4741 aacataatta tgcataggag tattatataa tgaagaaata aaaaaaaata ggtcacttga
     4801 cccgaaataa aaaagcattg aataattatt attcaatact tttttgaaat tatacttatg
     4861 ttgtaggaag attctttaat acctctcgct gattaattac actgaatctt ttgttttcat
     4921 aagcaattag atgttcttgt acacattcat ttatagcacg attaatgctt ctaatcggag
     4981 cgtttacttc gctagcaagc tgttttttgg taagcagatt taattcactt ctataatagt
     5041 gtaaggatat acacctcaga agtcgttctt taacacttaa agatgataat tcttcaatta
     5101 attcagcatt tacaactaat ttactagata tttctcgaat cagaaatttt gtagcttcaa
     5161 aatctcccct taaccagttt aaaaaatcat ctctatgaag tttatataca ttacaatctg
     5221 tgaaagcgac aatttcaacg ggttttttcc catcattaaa ttgttccatt tctccaaaaa
     5281 aactaccact tctataaaga tgaatagttg agatgtttcc tttcacattt aagatgaatg
     5341 cttctgcaat accgtcaatc ataagaatta catattcatt ttcttgctca gcaaaaagaa
     5401 tagtctcttt ctttttatac gtttttttaa caagcttctt tttaatatct tcattcataa
     5461 aatcaattaa atgcatattc atctctcctt accaaatctt tgattaaatt atatcatctt
     5521 tcaattagtt aggtaagata gtaaaaatat atttacatag aaactattcc attattattt
     5581 tccagttgtc atttttctgc aaaaacttat cactgtcacg actggagata ccagtaagga
     5641 atgttaggat atcaatcgct aaactttcag aatccttttt acggctcaaa atgacgtaag
     5701 ggtcaagcaa tcctttgttt tcatcatcac tggttaaatt gacaatgttt atctcatggg
     5761 ctatttctgg tagcgtttct ttccatttgc cgcgttttgc tttagggtca actatgaccg
     5821 cttgtccgcc gaatagtacc gcatagtaaa caagcatatt gttactgaat aatttaccac
     5881 cacccaatga accgataaac gcagacgcta acgcattggt aatagaaccc tttacccctt
     5941 gactagcaag ggcaggtttt agatagacat tctttccagt gttcagattg tagccaagat
     6001 agatatcctc atgttcgcca agggtttgtg tcgctccaaa accaagtcca gccaaaaaat
     6061 ccgaagtgac atattgaata taatcattga tgtaacgttt gctgacagga ataaattcgc
     6121 catgcaatcc caacatatcc ccaaagggac gcacgagctt gattgataag tcgtcgtaaa
     6181 agtctttgac ttcataacaa cgccttttta gctcgtcaag atcctttgta gatacacgga
     6241 caacatagct aagtttatac atagattctt tacagttaaa agtctgtttt taaaaaaatg
     6301 tataaattat aaatcaaaat atagatatta tcaatgaccc actaggtgca aaaagcattg
     6361 atatggttaa attatcagac gctaaaagct gggctatccg aatgagtgaa gatgggtgtg
     6421 cttataaatc cattgacaat tataagcgct cactgaaagc gtcgtttttc atggcaattc
     6481 aagacgactg tattagaaag aatccattta attttcagtt aagtgatgtt ttggaagata
     6541 caacagaacc aaaaatcgct ctaacaccag aacaggaaat gggcttatta tcctttatgg
     6601 agagcgataa gacttattgt aaatattatg atgatgttgt tatcctctta gaaacaggac
     6661 tacgcatttc tgaattatgc ggtttgactt tagaattgga tatgaaaaat aaagccatta
     6721 tggtagacca tcaattgttg agtgatacta agattggcta ttatgtagaa gttccaaaga
     6781 ccacacaggg taagcgagaa attccaatga ctgaaagagc ttatcaatca tttgaacgta
     6841 tcgtggaacg gcgtaagaac gctaaagcag aaccaatcat tgttgatggt taccagaact
     6901 tcctatttct taaacaagat ggattaccaa aacaagcaac tcattatcaa agcatgttaa
     6961 gagcgttagt caagaagtat aacaagaccc ataaggacaa gctaccaaag attacgccac
     7021 atacctttag gcatacactc tgtacaaata tggctaacaa aggaatgagt cctaatatat
     7081 tgcaatatat tatggggcat aaaaacatta ctatgacgct tggctattat gcacatggtt
     7141 catttagttc tgcgagagct gaaatggaac ggttataagc ctagtagtat gactagagtt
     7201 tactacttat ttactacttt tgatggcatt cttatacggt taaatgccaa cttatgcaaa
     7261 gtatcttcca aaaagaaaat gccgatactc cttgtagata caggatatat cgacatttac
     7321 caacttatga aaagataatc aaatttaata taagtttttc aaataaaaag ataccaaatt
     7381 ctttttcgag aatctatata acattactta agcttggttg gggtataaac aattgagtag
     7441 ctacttttgt atggtgttca actttagtca aaactcttaa gtaatgcaaa tgatttagat
     7501 tcattaatat tcaatctctt cataagtagt gaaattcgag ttatttctgc ataattatac
     7561 tatgatttat agattaattc tattagcatc cagaataata ttgtttatat gaataaaatt
     7621 taaagaatat tgtgaaaatt atagaatgtt aaaataaatt taggtaaaaa agaagtgttt
     7681 gtatattata gagtaccttt tctaaaatat gtgggattgt tttaagaatt agaataaaaa
     7741 ttatttttaa attagtattt ttataatgta tagtattttt aatgtataat taaattgtgc
     7801 agaaaataga atttaaagtc ctatagaatt tctgattatg gaggagaata tgaaaagaaa
     7861 agttcaagta aaaaatatta ctattggaga aggtagacct aaaatttgtg taccaattat
     7921 tgggaaaaat aaaaaagata ttataaaaga ggttaaagag ttaaaagatg catgtttgga
     7981 tattatagag tggcgagttg atttttttga gaatgtagaa aatattaaag aagttaagga
     8041 agttctctat gaattaagaa gttatataca tgatatccct ttattattca catttagaag
     8101 tgtagtagaa gggggagaaa aattaatatc aagagattat tatactactt tgaataaaga
     8161 aatttctaat acaggtttag tagatttaat agatgtagag ttatttatgg gagatgaggt
     8221 catagatgaa gtagttaatt ttgctcataa aaaagaagtg aaagtaatta tatctaatca
     8281 tgattttaat aaaactccaa aaaaagaaga gattgtatct cgtttatgta gaatgcagga
     8341 attgggcgca gatttaccta aaatagctgt tatgccgcaa aatgaaaaag atgtgttagt
     8401 gctacttgaa gctacaaatg aaatgtttaa aatttatgca gatagaccaa taataacaat
     8461 gtcaatgtca ggaatgggtg ttataagtag attgtgtggt gaaatatttg gttctgcatt
     8521 gacttttgga gctgcaaaaa gtgtttcagc accagggcaa atatcattta aagagttaaa
     8581 ttcagtactt aatctattac ataagagtat taattaaatt aattatatag aaatattaga
     8641 cggtattttt gaagatataa attaaatata aaaaactttt cagatagtaa aatttaaatt
     8701 gggcaattat atctgaaaag caactattta gtgtgtattt tgaaagtgat atagaaaatc
     8761 ttataaatca actatggcat atttaaaata tgccattaaa acctactaga cattagatta
     8821 tagatggtat actagcattt ctattattta tagatagtaa gttttcataa taactgtttt
     8881 atctaaagta gaagatatat ttgtattatg tatatttgtc attatttttt tgtaattatg
     8941 tatattaatg gttatatatg taatataata ttatcactct attttttgta tattgtacat
     9001 tacaacattt atatctatca tcatcaatta cagtagaatg tatgggatac ttattttcac
     9061 ttaaccaata tttttgcata ttctttgtcc aagactttgg aaacttcaat acttttttgt
     9121 ttattataac atcctcatct tcaaaaatat taacttctct ttctattttt atttttctgt
     9181 tgtatatctt attgatagat tctttgtcga gttttatgtc cctatacata tcagggcaat
     9241 agcaacaaac ataatttctt ttttttcgac gtttaaattt cacaactttc tctatgctaa
     9301 tttctaattt ttttgtacca tcttttataa atttaatatc ttctatgttc tcgcttgaaa
     9361 aaatttctgt caatcttaaa gtatctacag tttctctaat tatttgcttt ttataagaag
     9421 agaatccata tccaaaagaa aggtttaatt tattagggat gtagccattt ttatatttaa
     9481 acatcttaat tttttccaat tcagcattat ctttaaattt caacttttca tacctaaaag
     9541 ttactttgct aaaaagagta tattctacat caatatattc ttttgcaatc ttaaatttat
     9601 cgctaccttc aatcacaatc atagcctatc tccaatcgtt ttatattaaa ataaggccat
     9661 agaacatggt ctaatagtac tcattagcac tataagacaa tgttaacaca aaaagacaaa
     9721 aaaaggttat ttatttcaaa taaccttttt ttttatataa aatataaaga attatatttt
     9781 attctttttt atgatgtcta taatctgtga tatcagattt agttctcttg aatttaagtc
     9841 ttttaactct tcatttatat cattttctaa atatgttttt ccagtggaag taatatcttt
     9901 tataagaaca tctgcagaaa tatttaatat atttacaaat tttatgaatg ttttaagact
     9961 tggattcttt ttaccacttt cgatttgagt tatataccaa ggcgatactc caaccatatt
    10021 tgcaaattct tttgaagtta aatttgcttt tttccgttct tctttaagtc ttttacctat
    10081 ataattcatt attgttacta tttccctcac ttatatttct ttgtatttaa tgatgcactt
    10141 tattaactct agcattgcaa tttgttcttt ttcagttaat ttattaatca tttcataaca
    10201 ttctaaaggt acagagttat caacgccttc tgttgtaata atatttgcaa ataacaattc
    10261 aagtggtatg tctaaagcag tagcaatttt cattataata ttgatagatg tatttgtctc
    10321 agctctttca atctgaccaa tatatgttgg atgtaaacct gccttttctg ctagaaactc
    10381 ttgactatat cccaattttg ttctatagac ttttattcta tctcctatta tttttcctat
    10441 atttgccaat tattatccct ccactttaga ttatattcat agtttaagtt gttttatata
    10501 gactatttat acttttttag tctaaatata gactatatat gtttttatac tgaaataagt
    10561 gctatttatt tttgtatagt ctgtatctac aataaacaaa tatggataaa tcatattttt
    10621 aattagag
//
LOCUS       DACXMZ010000136        10621 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.137, whole genome shotgun sequence.
ACCESSION   DACXMZ010000136 DACXMZ010000000
VERSION     DACXMZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10621
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.137"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            51..899
                     /locus_tag="JD016_13080"
     CDS             51..899
                     /locus_tag="JD016_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAU5000565.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            978..2900
                     /locus_tag="JD016_13085"
     CDS             978..2900
                     /locus_tag="JD016_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HAU5000566.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            2982..3845
                     /gene="aroE"
                     /locus_tag="JD016_13090"
     CDS             2982..3845
                     /gene="aroE"
                     /locus_tag="JD016_13090"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAU5000567.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKIKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIKSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4045..5262
                     /locus_tag="JD016_13095"
     CDS             4045..5262
                     /locus_tag="JD016_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAU5000568.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5495..7363
                     /locus_tag="JD016_13100"
     CDS             5495..7363
                     /locus_tag="JD016_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HAU5000569.1"
                     /translation="MVENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANN
                     LVDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKE
                     LIYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYL
                     FNLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYL
                     EKRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFL
                     IAINVLKDENIECNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDV
                     NLSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEK
                     DKLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLS
                     LEELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIA
                     VAGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYIL
                     SNLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7541..8905)
                     /locus_tag="JD016_13105"
     CDS             complement(7541..8905)
                     /locus_tag="JD016_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAU5000570.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9084..10427)
                     /locus_tag="JD016_13110"
     CDS             complement(9084..10427)
                     /locus_tag="JD016_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAU5000571.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10500..10601)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         3919 a         1355 c         1831 g         3516 t
ORIGIN      
        1 atcaaaaata ctattaattg taaagaaaat aaagggggca aatttaagct atgaataatc
       61 aaaaaaatct taatattcca attacaatta gttcttatac attgggaaca gaagtttcat
      121 tccgtgatag agttcgtatt gcaaaagaag ctgggtttga tggtattgga ttgcgtgctg
      181 aaaattatat tgatgcaaaa aatgcaggtg ttacagatga agaaatgatt gcaattctta
      241 atgaatatga tatgaaagtt acagaagtag aatatataac tcaatgggga agagaagaag
      301 atagaacgga agctcaaaga gaaaaagagc aaaatgttta tcatatggct cacttattta
      361 atgttaaaca tataaattgt ggattattag agatgatacc agaagaacaa attataactg
      421 cattgggaga attatgtgac agagcaggtg aacttattat aggtcttgag tttatgccat
      481 atagtggtgt accagatttg gctactgcat ggcgtgttgt gaaaggatgt aatcgtgaaa
      541 atgcaatgct tatattagat acttggcact gggctagagc aaagcagaca gcagaggacc
      601 ttgcacctgt tccagcagat aagatagtat caattcaagt gtgtgatgtt ttagaaactc
      661 cttatgaaaa acttagggat gaatcactac atgaccgttt ggctccaggt gaaggatatg
      721 gaaacacaga ggaatttgtg aaaataataa aagaacacaa tatatcacca agagtcatag
      781 gggcagaagt aatatctgat tctctaatat ctaaagggtt atccactgct gctgaaactg
      841 tatttaatgc aactaaaaaa gttttggata aagcatggtc tgaagtatct ccaaaataaa
      901 cttatataat ataaaaatat acagagtatt ataatataaa acttttaaat ataaaacttg
      961 aaaggaagga aaaccttatg aagattagtt ctatgtttac acctataaga attggttcaa
     1021 tgacagttcc aaatcgtttt gtagttcccc ctatgggaaa caattttgca aacacagatg
     1081 gtactttaag tgaaacttct aaggcttatt atcttgagcg tgctttaggt ggttttggat
     1141 taataactat agaatctagt gttgtagaca aaaaagcaaa aggaggtcct cgtaagcctt
     1201 gtttatatga tgatagcaca atagatagtt ttaaaaatgt aatagatgct tgtcacgatg
     1261 caggttcgaa ggtatctata caattacaac atgctggttc tgaaggaaat gaaaaagtag
     1321 caggacatcc attaaaagct gcttctgcaa tacctgcatc aaatggtcgt aatacaccac
     1381 tagcaataac aacagaagaa atatatgaac ttatagaatc ttatggagat gctgcattac
     1441 gtgcacaaaa agctggtgca gatgctgttg aagttcactg cgctcatgga tatctggtca
     1501 gtagttttat atcacaaaga acaaataagc gtgtagatga gtttggtgga tgttttgaaa
     1561 atagaatgag attacctcgt cttatcattg aaaatatacg taaaaaagta ggaaattctc
     1621 tagcaatatt atgtcgcata aacagtactg atgatgttcc aggaggaatt gatgtacacg
     1681 atagttcagt aatagcagca tatctagaag attgtggaat agatggactt catgtttcac
     1741 gtagtataca tatacatgat gaatatatgt gggctcctac gactttacat gctggattca
     1801 gtgcagaatt agttactgaa ataaaaaaag ctgttagtat accagtaata acagttggac
     1861 gttatacaga gccacagttt gctgagttaa tggtaagaca aggaagatgt gatttagtag
     1921 cattcggtag acaaagcttg gctgatccag aaatgcctaa taaagctaaa aatggaaaac
     1981 tagacgaaat gataccatgt attgcatgct tacaaggatg tgtaccaaat atgttccaag
     2041 gcaaacctat agcctgtctt gcaaatccta tattaggaca tgaagcagag cttaagccag
     2101 ctgaaattag caaagaagtt cttgttgttg gtggaggtgt tggaggtatg cttgcagcat
     2161 gggtatgtgc taaaagagga cataatgtaa ctttagttga aaaatctgaa gttcttggtg
     2221 ggcaaatgcg tttagctgca tatcctccag gaaaaggaga tattactaac ttagttagaa
     2281 gttatatttc aaaatgtaat caatatggtg taaaaatatg tactaataca gaagctacag
     2341 tagaacttat aaaggagaaa tcacctgatg tagttattat agctactgga gcaactccac
     2401 ttgtacttcc tataccagga atcaatgatt caggattaat tcatgctgta gaccttcttg
     2461 atggtaaaaa atcttgtggg aaaaaagtac ttgttgttgg aggtggaatg gttggatgtg
     2521 aagtggcagc cttccttgga gaacaagaac atgaggttac tgttattgag cttagagaag
     2581 aagttggtgc agatgtaatt tcagaacacc gtaaattcct tatgaatgat tttgaaaatt
     2641 ataatataca taccattact ggtgcaaaag tttctaaatt ttatgatgat ggtgtcgact
     2701 atttacttac agatggcaca gagcatgatt taaaaggttt tgattcagta gtacttgcta
     2761 tgggttctcg taattacgac ccattaagtg aagttataaa agaagttgta aaagaaactt
     2821 atattgttgg tgatgcagta aaagctcgta gggcattaga tgcaacaaaa gaagcttttg
     2881 aagtagcatt aaatatataa ataataaata aatttttctt tgtaaataac tgaataggtt
     2941 atttgcaaag aaaatgataa tattgggagg agattatagt tatggaaatt tcaggtagaa
     3001 caggattatt tgcattaata ggtacaccgg ttggtcactc taaatcacca gtgatgtata
     3061 attatagttt taagaaatta gatttagatt atagatattt agcctttgat atcacagtag
     3121 ataagataaa agaggcactt ttagcaataa aaacttttaa tataaaagga gccaatgtta
     3181 ctatgccatg caaatctgct gttacagaat atatggatga actttctcca gcagctcgta
     3241 taattggagc atgtaataca attgtaaatg acaatggaaa gttagttggt catataacag
     3301 atggagtagg ctatgttcgt aatttaaaag aaaatggggt agaagttaaa ggtaaaaaga
     3361 ttacaattat gggtgctggt ggtgcagcaa ctgctataca agtacaatgt gctttagatg
     3421 gagcaagaga aatatctatc tttaatccta aggatgattt ttataaaaga gcagagcaaa
     3481 cagttgagaa tataaaaaaa gatgtaccag aatgtgttgt taatctatat gatttagaag
     3541 atacaaacaa actttatgag gagataaaat caagtgatat attgacaaat gcaactctta
     3601 ttggtatgaa gccttatgac aatgaaacaa atataaaaga tactagtgtt ttaagaaaag
     3661 acttagtagt aacagatgtc gtttataatc ctaagaaaac aaaaatgatt gaagatgctg
     3721 aagctaatgg atgtaaggct attggtggat taggaatgtt attataccaa ggagcagagg
     3781 cttttaatct atatactgga ttagaaatgc ctgtagaaga agttaatgaa ttatgcttta
     3841 aatagataag agtaatatct taaacaatga aaaaacatac gtatttcaat atttttaata
     3901 ataaattaca aattgtcata gtgggttttg cctcatagtc cagttaaatt tgaagcttta
     3961 actgactata ctttaaatcc attatgggga tgagtttatt gtacacaaaa atgttaataa
     4021 attaacaaga atgggagaaa aattatgaac aataaaaaat attatcctac tgccatagcg
     4081 ttgtattttt catatttttt acttggtatt ggaatttcaa ttttaggaca atacaaacca
     4141 gaattttcat caatgtgggg ggctaaaaca ttatcagatg gaacattgga tgtaagtatt
     4201 gttttagctg ttattgctgc actaggatta gggagactta tatcttatcc atttgcagga
     4261 cctatttcag ataagtatgg aagaaaagta agtggtctta taggaaactt tctacatgca
     4321 attttctttg ttggaattgt attttcacca aatttttata tagcatatgt atttgcaata
     4381 attggagggg cagcaaattc attcttagat acctgtgtaa ctccttcttg tatggagata
     4441 tttgctagtt taggaacaat agcaaatatg tttactaaat ttacaatagc tttagcacaa
     4501 tttttgcttc cttttataat tggatttgtt gcagcaaatt caatatcatt taaagttatt
     4561 tttatagtaa cagcgattct tattgtagta gatgcctttt tgatagctgt gttaccattt
     4621 ccaccagcta ataatgttat tgataacaaa ggcaaaacag ttaaatcaga gaagatgaaa
     4681 tttacaccaa caagtatagc tcttgtttgt attggattta catgtacatc tacatttgta
     4741 ttatggttaa attgtaatca ggaattgggt aaattgtatg ggatggctga ccctacaaaa
     4801 attcaatcct tttactcaat gggagtaata tgtgcagtat taataacttc tttacttatt
     4861 aaaaaatata ttaaaccaat aagaatatta gttatatatc ctataatagc tttacttatg
     4921 ctattagttg tatattttgt tcaaactcca actatttgta tgttaggggg atttgtaata
     4981 ggttattcag cagcaggagg tgttcttcag ttaacaacgt caactgcaaa tgaaatgttt
     5041 ccaactaata aaggaaaaat aacatctata gttatgattg catctagtat agcaaactat
     5101 gttattttaa atatagcagg tattattaca aaatcagggg gagttaatgg accaaaatat
     5161 gttgtattgt ttaatgtggc aataacattt gttggaattt tacttgcatt gtttgtaaac
     5221 atgagatatg aaaaagaaaa agtgtatgat tatgatgtct aatattagta taatatatgc
     5281 atagagaagt ctaaatctag gatagatttt tattaaaagt aattaaaatt aataaggata
     5341 ttatttacag ttataagata gggtatttac ataattaata ataaggatat tactagaatt
     5401 tcaatttcta gtaatatttt tttgcgtata aatgtaaact tgacgaaata agtagtataa
     5461 tcaatataaa ttacatcatg taaaggagga taatttggtg gagaatgttt ttaagcgatt
     5521 gcaggaattt aatggttatg atggatacaa agaatcattt gaaatgaatt acctatgtat
     5581 atatgaaagt ataccgttaa gagaacaagt agagcttgca aataatctag tagatgaaat
     5641 tttaaatatg tataaaagtg aatctaatga aatttattta ttggaggata gtaattctaa
     5701 gtctttaatt tgttatttcg aaatttttat gaaaaagata aatactttgg taaaagaaat
     5761 gattattgat gagaaatggt tatataaatt aactaaagaa ttaatatata agagtaaaaa
     5821 agttgagtat gtaaaactag ggttagtttt aagtgagaaa tatctaaatg tagaaaactt
     5881 aagagaagtt gtagatacat tttctaaatc tggagaatac gtattttatc tgagtaatac
     5941 tattaaaaaa cttgaatttt acaatacata tttatttaat ttatctaaaa aggctacagg
     6001 ttcaataaaa gtatttgcta ttgttaatat ggaaaattta gattctaaaa taaattccta
     6061 tctaattgaa gatggttata aggatacaaa gtacgaacga ttattgatga actatattat
     6121 ttcaattgta gatttgaatg aatatcttga aaaacgagat ttagataaag aaaagataaa
     6181 taatttagca cgtcttatat gcaattactt gctaagtgta gaatttaaat atataggtaa
     6241 taaattagaa ttagtgaatc gatttttacc tactgtagta aattatggga ctaactttga
     6301 aagtttatat tcaattttcc taatagctat aaacgtctta aaagatgaaa atatagagtg
     6361 caataagatt gaatttgaaa aagaaataaa tgatatcttg ctcagtgaaa aatggaaaaa
     6421 tatatatttt gaagcattaa gagatgctag tggaaaaaca gaagatatta taaaaatgtc
     6481 agagatttat gatgtgaact tatcttttga tgacttgttg ccatatctga atagagatat
     6541 tagagatttt gaggtatatt ggcatatatc taaaaaggga acgacaagtt ctagattaaa
     6601 attattaaat ttttttgaag agacgtttaa aatagatgat ttaattggaa aaatgaaaga
     6661 tatagaaaaa gataaattga ctcaagaata ttatgatgat atgttatttt ttattgtgct
     6721 taaaggaagt aaatcactat atcctgaagg aaagaatata tctttaaaag gaatatttgg
     6781 aaatataaat gaagtaagaa aagaatccat aaatatctta aaaagataca gagagaaact
     6841 atctcttgaa gaattaaaaa ttgttaaaga agcgtatgaa aaagaaaaaa atgttatatt
     6901 gaaggatgaa ttaagaagag tactatatga aagtaataat ttaaaaaaag aatttgtaaa
     6961 tattgaaaaa ataaaagtag atgaacatgg taaggatatc tatttgactt caatagctgt
     7021 tgctggttca aggtttagaa atagagaata tcttgaaaaa gaattggaga aaagtaagat
     7081 atactattta actagagaga aagataatct atatgatgaa aaagctatta aaatagttgg
     7141 tgaaactgga tatgtgatag gttatgtccc aagaaaagaa aactatattt tgagcaattt
     7201 attagatgga ggaaaactgc tgtattgtag agtaactgaa tataatttat atgaagattg
     7261 tatctacgca aatgtgtact tgagttataa agatgtaatt gaaacagttg aaaactcttt
     7321 aaagatggtt ttagataaga gtagaataaa acttataaac taagacgttt atatattgct
     7381 tagatgatgt aatcttaact gattggttat tttttctgga attatggtgt attttatgag
     7441 caatgctaat gaatataaaa taaaaaaaca attgttttgt atatatgatg tatataaata
     7501 tatttatata ttcatattta caaaacaatt gaaatttatg ttattttata attttaaatt
     7561 tagatggaat tttaacttct gctttatctt ttattttaga agcttgtttt ttaactttat
     7621 ttaataattc agattcatct acaactaata cttttctttc cttcatcaac catttaccat
     7681 cacaaatagt agagtctaca ttttcactag tcatagaata aactatattt ccaattggat
     7741 tatgcattgg taaacaatga attgtatctg gatttagaat aatcatatca gctttttttc
     7801 ctacttcaag actaccaatt tctttttcta gtaaagcaca ttttgctcca tttatagtag
     7861 ccatttctaa gacttcttca gctggtacga cagttgggtt taaagttctt cctttatgta
     7921 ttagtgatgt aagatacatg tccctcataa ggtccattct attatttgaa ggagctccat
     7981 cagtaccaat tgaaacaggt attccttttt ctaacatttc aggaattgat gcaaatccta
     8041 gaaccacttt catagctgaa cctgggtcat gagaaacttt tacatcatgt aatctaaata
     8101 aatcaatttc cttatgagta agccaaactg tatgagcagc taaaagattt ggtcctaata
     8161 cacctagttt atcaaggtat tcaactgttc caactccatt gttttgcttt acaaatgaaa
     8221 tttcatcagc tatttcagca atatgcatat gtatacctgt attgtattta tcagctagtt
     8281 ctttagttct aacaagtaat tcttcagttg tattaaaaat agttcttaat gcaaaccata
     8341 ttttaattct ctcattagat gtattattaa acttatggaa taagtctaat tgagtattga
     8401 tttcctcatc agtagttttt tgccatgcct caggaagacc atctcctgta tccataactg
     8461 atttagctaa acaggctcta agtccagttt tatcaacagc ctcaaccata gcatctacat
     8521 attgaccacc agattctaaa aatgtagtga caccagattt tatcaattca acacaacatg
     8581 cagtagatga aatcaaggaa tcttcataat tgaagctact ttcgtatggc caaattctct
     8641 ctctaagcca agttaaaagt acaacatcat ctgcaagacc acgtcctaat tgttgagata
     8701 agtgaacatg cgtattgact agccctggca ttagtatttt tcctttagca tcatatatct
     8761 ctgcatcttt atctacaata ttaatatcaa acttaccaac ctcttttata atattatttt
     8821 caataagtat gcttccattt tcatatacct ctctatctgc attcatagta attagatatg
     8881 cattttttat aagtaattgt gacataaaca ccactccttt tctatatatt tttatatcta
     8941 tatattatga taataaattt tctatacaaa gttaatagca aaatcaattt acatataaag
     9001 tgattttgct atttataaat ttaaaataaa taagatttta ttctatataa tttaaaatat
     9061 tgacctagaa ctatttaaaa atattaactt agaactaagt ttacaactgc aagtaaacct
     9121 ataatccaaa gtgtaatact gattttatca gtttctttag ctactacatg cattattata
     9181 tatgaaataa atccaaagca taaaccaata cttatactat aagttaaagg catcaataaa
     9241 actgtaacaa aagcaggtac agctattttg ctatcagcaa aatcaatctg ttttatgctt
     9301 tcaaacatga atacaccaac cattacaagt gcaggagctg cagcgtaagt aggaactata
     9361 ccaagtaatg gtgtaaatat aagtgtagct aagaataatg ctgctgttac tattgaagca
     9421 agtcctgttc ttgcacctgc tgcaatacca gaagcagatt ctccaaatgt tgtaacagta
     9481 ctagttccta agaatgagcc tattaaagta gaagaaacat ctgcatataa cattcttcct
     9541 aaatctttta tttcaccatt ctcatcttca agacccattt gtttacaaca agtaataaga
     9601 aatgctaatg aatcaaacat gtctataaac atgaaagaga atataggtcc aattaatgag
     9661 aatttcaatg cacctacaat atctaattta aaagcaactg gtgctatact aggaggtgtt
     9721 gagattattg ctgttggcaa ctccacaaat ccaaatataa cagatattat tgttgcaact
     9781 actatactta tcaatattcc acctttaact tttttatatt ctagtattgc cattaagata
     9841 agagcaaaaa gagctattaa aactgttggt gtaaaatctg caagtgaaac tatagtgtct
     9901 ggattagcta ttataacacc catatttttt aaaccaacga aagcaaggaa aagacctata
     9961 cctgctgaag atgctactgc caattctcta ggaattgcgt ctgcaattgt ctttcgaaga
    10021 cctgttactg cgagtgtaaa gaagacacat ccagatagaa aaactacccc aagacctgtt
    10081 tgccatggga ttcctgcacc tataaccaaa gagtatgtga aaaatgcatt taatcccata
    10141 ccaggagcta gagcaaatgg tgcatttcct aaaatcgcaa atataagtgt accaattgct
    10201 gtagctagta tagtaactgt tataagggcg gctttatcca ttccagcttc agataatatt
    10261 gctggattta cgaaaactat gtatgccatt gttaagaatg tagtaactcc acctattact
    10321 tctgttttaa ggctagagcc acgttcgcta aatttaaaca ttttgtctaa aaagcttaat
    10381 gatgaattat ttccacctgg ttgtacattg ttagtgtttg gtaccatttt tacctccaaa
    10441 aaactttaag atataaacaa aaaaatatat attcatatag atagaataga acatatattc
    10501 agagttgaca ttctattcat agaaaaccca tttaaggtga gtttgtagaa actattagac
    10561 cgtattacta atattatatg aacattttct atttgttttt ctgttgtatt atatcgtaat
    10621 a
//
LOCUS       DACXMZ010000137        10614 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.138, whole genome shotgun sequence.
ACCESSION   DACXMZ010000137 DACXMZ010000000
VERSION     DACXMZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.138"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..377
                     /locus_tag="JD016_13115"
     CDS             <1..377
                     /locus_tag="JD016_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAU5000572.1"
                     /translation="LVQEEIDPLQNQGEGLGLAPIGHRVTVEGVTSTTINISAEITYK
                     SGYTWENIKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDITNTMI
                     NDVKSNLTIDSNSIVVRGEVVG"
     gene            370..987
                     /locus_tag="JD016_13120"
     CDS             370..987
                     /locus_tag="JD016_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HAU5000573.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEVTIF"
     gene            999..2003
                     /locus_tag="JD016_13125"
     CDS             999..2003
                     /locus_tag="JD016_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAU5000574.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLDSK
                     IDTTKTELTSNIETAKTELNTRIDTENEKQNIKIDNIIAGGVNVSHTHIIEVADWTLN
                     NETNMYEVTINHPLLTKRILIALYDEIGEALTPNARAIDDNSILVRNEENIKMYVYLI
                     NGNAETHFINATVDDNRVSEMTTYSSKKIEDRFLNLEEKLSGGLSNIATSVNELITYC
                     "
     gene            2018..3346
                     /locus_tag="JD016_13130"
                     /pseudo
     CDS             2018..3346
                     /locus_tag="JD016_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429874.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3668..4015
                     /locus_tag="JD016_13135"
     CDS             3668..4015
                     /locus_tag="JD016_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000575.1"
                     /translation="MNKANRIIYDQAGKIWLQTGEATGDIQDFTTITELNFIDVEYGS
                     IDYTKQYIESINPVTKEPVLKDIEIVLTDEQKRLEALEKELSMLKEENKNRDSEIVNT
                     AFEVENIRLNSNL"
     gene            4231..4824
                     /locus_tag="JD016_13140"
     CDS             4231..4824
                     /locus_tag="JD016_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000576.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTENKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPATSEQTFQDYANYITGFKNTIVSKDNTINSLRNRYQ
                     STSGFFIYDKEHYPNGIVVGFKPDIVSYTTTNLVGEKYYCIYTPLFYFFYNARTKETC
                     FDSTDSHRGIDIKDNSWLPKGPVNGGAGYDYIAVKYR"
     gene            4884..5114
                     /locus_tag="JD016_13145"
     CDS             4884..5114
                     /locus_tag="JD016_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000577.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAVQKGIF"
     gene            5130..5390
                     /locus_tag="JD016_13150"
     CDS             5130..5390
                     /locus_tag="JD016_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAU5000578.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPIILLVL
                     GIGFSIWMLGFSPNAVLQGVICWGISIGINQTYKQLKEENKQ"
     gene            5387..6184
                     /locus_tag="JD016_13155"
     CDS             5387..6184
                     /locus_tag="JD016_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAU5000579.1"
                     /translation="MKIAIVPGHTLTGKGTGATGYIDEGKENRILTDLIVKWLKQGGA
                     TVYTGKVDKSNNYLAEQCEIANKQNVDLAVQIHFNANKTTLNSMGTETIYKTNKGKVY
                     AERVNKKLATIFKNRGAKSDVRGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKNINNEGVKQMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIKDYV
                     PGQTQNLYVIGGAACEKIGSITKEKYTMINGSDRFDTLYKALDFIDR"
     gene            6597..7625
                     /locus_tag="JD016_13160"
     CDS             6597..7625
                     /locus_tag="JD016_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000580.1"
                     /translation="MNKELVYGEIDEKNLDVSRISHEELKALFNLMNGKPDSMLRIFP
                     RDVNVEIKDLYSLHEKIKEKLQHYIVAALIVNISVKFDNNTFKEFSDWIEFQNCNWSS
                     SHYLENITIKYDFFVDFKEYGVPQRHTLVIRLSSGMKPQQILQLIVSGQIEEIDSIDK
                     SLSPVTCKVDFTNHLLGDELINIIEEWDKSIQKVVSESGKTMKFIRKYRKGISAVIEN
                     IIPLFGLIVLVSIVNFNLSHFNIDTLVQLSISHIQKLIMLTLLFSLIYKVIKNIGEFL
                     SNKFLTEIYEYGDTHIFNITNGDKIKREKLDIKNKKHEKGIKSSLSLTILINVICGIV
                     ATYICTIF"
     gene            complement(8220..8408)
                     /locus_tag="JD016_13165"
     CDS             complement(8220..8408)
                     /locus_tag="JD016_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000581.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHGYSKK"
     gene            complement(9059..9235)
                     /locus_tag="JD016_13170"
                     /pseudo
     CDS             complement(9059..9235)
                     /locus_tag="JD016_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589386.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     regulatory      complement(9290..9387)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            9703..9984
                     /locus_tag="JD016_13175"
     CDS             9703..9984
                     /locus_tag="JD016_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAU5000582.1"
                     /translation="MKEKYKWRKSTTEILLGRLVKMRILKKKRIGFQLNYEVLVTKKE
                     YLDVIKEEKDVSKYDNFFTQVFTTIHKKEEKTEEQIKVFIESMQKLGKK"
     gene            10024..10410
                     /locus_tag="JD016_13180"
     CDS             10024..10410
                     /locus_tag="JD016_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAU5000583.1"
                     /translation="MLNKKLPDSEFKIMKYIWNTGYKTVISKDVADEMEKIYKWKQTT
                     TLTLLLRLTKRGFLASQKIGKHTHYTILIKEKEYLKSETKKLFGGLHNNPLSQLISKL
                     HDKEEVSIDKIDALEEWLRSLQEDEE"
BASE COUNT         4331 a         1163 c         1798 g         3322 t
ORIGIN      
        1 atttagtgca agaagaaatt gaccctctac aaaaccaagg agaaggtctt ggattagcac
       61 caataggtca tagagtcaca gttgaaggag ttacaagtac aactataaat atatcagcag
      121 aaataacata taagagtgga tatacatggg agaatataaa atcaattgca gaagaagcaa
      181 tagacgacta tttaaatgaa cttaacatga gttgggaaga tgaagaaaac ttaatagtcc
      241 gtatatctca aattgaaact agattactta gtatagatgg agtgttagat attacaaaca
      301 caatgataaa tgatgttaaa tctaatctaa caatagatag taacagtata gtagtgagag
      361 gtgaggtagt tggataaaga gattaatcta ataaattact tgccacaaat tctacaagat
      421 aaagaagaat atataaaagt atttaatgta gaaaataaag aaataaaaac actacatgat
      481 aaattaaagg acctatcaaa tgaccagttt ttagaggacc taactataag tggtataaaa
      541 agatgggaaa agataatgtc tataactcct aaaagtaatg agagtttaga agatagaagg
      601 tttaggattt ttagtaaata tataagtaaa ttaccttatt cagagagatt tttaaggaat
      661 tggctagata gtatagttgg agaaggtaat tatgaattaa ctattaataa tgctacttat
      721 aacatacatc ttgagagtga tgctagaaat caagattggt ttgaggaagt acattctttt
      781 gtaagtaata ttaagccatg taacatgaca ctagattaca ctagagtgct tataagcaaa
      841 gacaattata tgaattttgg tataacaacc ctaatgggtc aagaaataac tatataccct
      901 tggagtccac cagatataga aacttatgga gaaattgatg tattaactgg caatggagtt
      961 ggataccaag aggtaacaat attttaggag gtgatatatt ggctatagat aaaagttatt
     1021 acactataat tacagatgta ggaaaagcaa agatagcaaa tgcaagtgtc acaggtaata
     1081 aagtgggatt tgtaaaaatt caacttggtg atggaggggg gagtgagtat actcctactg
     1141 agagtcagac agctctcaaa aatgtggtat gggaaggcaa tatcggaaat acaactacag
     1201 atgaaactgc accaaattgt ataatattag agagtttaat accatcaagt gtaggtgggt
     1261 ttatgataag agaaatagga tatttagatg atgaaaataa tttaattgcc atttctaaat
     1321 acaaagagtg ttataaacct tctatagaac aaggtgcagt ggtagacatg aaggttaaaa
     1381 ctgtgcttat tgtatctaat gtaaataata tagaacttaa aattgaccct acgataattt
     1441 ttgctacact taaagacata caggacctag actctaaaat agatacaacc aaaacagaat
     1501 taacaagcaa catagaaact gctaaaacag agttaaacac tagaattgac acagaaaacg
     1561 agaaacaaaa tattaaaatt gataatatta ttgcaggtgg tgtaaatgta tctcatacac
     1621 atattataga ggtggctgat tggactttaa ataatgaaac taatatgtat gaagtaacta
     1681 taaatcatcc actattgact aaaagaatac taatagcttt atatgatgaa attggtgaag
     1741 cacttacacc aaacgctagg gctattgatg ataatagtat tcttgttaga aatgaagaaa
     1801 atattaaaat gtatgtgtat ttgataaatg gaaacgcaga aactcatttt ataaatgcaa
     1861 ctgtagatga taacagagtg tctgaaatga ctacttattc atctaagaaa attgaagata
     1921 gatttcttaa tttagaagaa aaattgagtg gtgggttatc taacattgca acaagtgtaa
     1981 atgagttgat aacttattgt taaaggagag tgagaaaatg cagactgaat ggaattttaa
     2041 ttatgctaat tatgtacaaa atgtttcatt gccacctgga cgatataaat tagaatgttg
     2101 gggtgcttgc ggtggtactc ttggtgtaag taattggact gaatgtgcaa aaggcggtta
     2161 ttcaaaaggc gaagttatat taaaaaaaag aactaactta tgtatctgtg ttggtcaatc
     2221 gggttatgaa aaacttgata ctggttcaac tcttaccaga actggattta atgggggagg
     2281 acgaacaaat gcacatagta ttaatggcaa tttttattct aaatacggtg gtggagcaac
     2341 tgatataaga acttattatc ctagagcgac ttgggatagt gcagaaggtt tactttcgcg
     2401 tatacttgtt gcaggcggtg gtggagggat gaaaagtaat tacgccgctg ctcgttctat
     2461 tggtcatggt ggtggttatg taggtactaa cggaattggt cgtgacagag atttttgtgg
     2521 tggtggttct caataccaag gtggaacaag ttacgataca gaagaatacc atggttcgtt
     2581 aggaaaagga ggttatggtg gaataggagt aggtggaggt ggtggttggt acggtggtgc
     2641 tggttcttat tctaatgaat gtggaggtgg tggaagtggt tacgcactaa ctaaagatag
     2701 ttataaacca caaggatata taccaacgtc tgaatattgg ctagaaaatg tagttatgac
     2761 tactggtggc aatactacta aagcagatgg ttacgctaag ataacactac tacaagcatt
     2821 gccattttta actatatcct catataactc aactacagca acattcaaag ctgaccacac
     2881 agaccctacg ctattaacta aaatagaaat atttatagat gacgtattaa aagaaactat
     2941 aacaacagat ttaacagaag agaaaacaat taactataca ttagaagata atgctctaca
     3001 cacacttaaa atagttgtta cagacagtaa taatgctaca gcagaaaaag tattaagtat
     3061 aagtaagaat ataatgccac tacctgaaaa tgtaaatttg caagatatat caacaaaatt
     3121 aacagaagtt aatgcagggt ttagaactgg taaaacaagt attataaaca ctttagcact
     3181 taaaaatata gacgcaagtt taaataatac cttagtagaa ttatcagaga aaataaaaac
     3241 aagttttgat agttctgatg ctagtgttga ggagttgcaa aataaaataa tagaattgaa
     3301 taatcagtta agtcaacgta aaagatgggc aagtgggaca gatactatta ttgaccctag
     3361 tgcattaaga tttaatcatc aagttgattt tgagccaaat ataataattt ttttcatagg
     3421 gcagcataat agcttaaaca tagatacagc aatctattat aaaaatgcta catggagtca
     3481 atctagttca tcaagtggaa gtttttttaa aattataaat ggagtgacag ttcatttcag
     3541 agttgcaatt aatatgatgt ttaacaattc ttttgatgtt tatgttatta aagatgacca
     3601 ccctgagata actggagcta caacatgggt taaatggttt gtttttgaat aggaggaatt
     3661 aaaaattatg aataaagcta atagaataat atatgaccaa gcaggaaaga tatggcttca
     3721 aactggtgaa gcaacaggag atatacagga tttcacaaca ataacagaat taaatttcat
     3781 agatgttgaa tacggaagta tagattatac aaaacaatat atagagtcta taaatccagt
     3841 tacaaaagag cctgtcttaa aagatataga gattgttttg actgatgaac aaaagaggtt
     3901 agaagcatta gaaaaagaat taagtatgtt aaaagaagaa aataaaaata gagatagtga
     3961 gatagtaaac actgcttttg aagtagaaaa tataagatta aatagtaatt tataggagga
     4021 aatgatatgt ataacttatt aaaattaatg atagaacaaa agaactatag cactaaggag
     4081 gatttacaac ataagatgga tgtattctat gcagtaaata ggattacaga agaacaatat
     4141 ttagagttaa caggtttatt aaataaagaa gaaacaccag tagaaccaac agtttaaggg
     4201 aggttctttt ttattgaaag aggtgattaa atgactttta aagagttagt taataaagtt
     4261 agaaatcttg tattagaagc aaagaatgta actatagaag atacagaaaa taaattcact
     4321 agtgaaaatg tagaaggagc attgaaagaa tgtatagata gagcagatga ggcttttcaa
     4381 gaagccgata gtggaaaaac acttttatca actgctatcg gctctcctgc tacatcagaa
     4441 caaacatttc aagattatgc gaactatatt acaggattta aaaatactat agtaagcaaa
     4501 gataatacta taaatagttt gcgaaataga tatcaaagta caagtggatt ttttatatat
     4561 gataaagaac attatccaaa cggaatagtt gtaggtttta agcctgacat tgtttcatat
     4621 actaccacaa atcttgtagg tgaaaaatat tattgtatat acactccttt gttttatttc
     4681 ttttataatg caagaacgaa agaaacgtgt tttgatagta ctgatagtca cagaggcata
     4741 gatataaagg ataattcatg gttaccaaaa ggtccagtta atggtggagc aggttatgat
     4801 tatatagcag ttaagtatcg ttaattgtcg aagacttaga taaatttcta agtcttattt
     4861 taacacaaaa taaggaggct tacatgaatg aagaactttt cgaagcagat ttaaaaagac
     4921 atgaaacaag aataaataaa catggagaag aaatagacga attaaaaata gcaaatatag
     4981 agtctaaagc agagttaaaa gcattgtgtg agaacttaaa ttcccttacc agcatgttaa
     5041 agtggctaat tggaactatg attacaacac tagtagggtt ctttatattt gcagttcaga
     5101 aaggaatatt ttaattagga ggataagaga tggataattt aataagtttt ataccagagc
     5161 agttgctaat tttagtagct gctctctcta ttataggtaa aggttgcaaa aaatataaac
     5221 aattagacaa taaatacatt ccaattatat tacttgtgtt gggaatcgga ttttctattt
     5281 ggatgctagg gtttagtcct aacgcagtac tgcaaggtgt aatttgttgg ggaatatcaa
     5341 taggtataaa tcaaacttac aaacaactaa aggaggaaaa taaacaatga aaatagcaat
     5401 agtaccaggg catactttaa caggaaaagg aactggagca actggatata tagacgaagg
     5461 aaaagaaaat agaattttaa ctgatttaat agtaaaatgg ttgaaacaag gtggagctac
     5521 tgtatatact ggaaaagtag ataaatctaa taactattta gcagaacaat gtgaaattgc
     5581 caataaacag aatgtagacc tagcagtaca aatccatttc aacgcaaata aaacaacact
     5641 aaactcaatg ggtacagaga caatatataa aactaacaaa ggtaaggtat atgctgaaag
     5701 agtcaacaaa aaactagcaa caatatttaa aaatagaggt gcaaaatcag acgtaagagg
     5761 tctttactgg cttagtcata caaaagcacc agcaatatta attgaagtgt gcttcgtaga
     5821 tagtaaagca gatacagatt attatatcag acataaagac atagttgcta aattaatagc
     5881 agaaggtatt ttaaataaaa atataaataa tgagggagtt aaacagatgt acaaacatac
     5941 aatagtttat gatggagaag ttgacaaaat ccctgcaact gtagttggtt ggggctataa
     6001 tgatgggaaa atactgatat gtgatataaa agattatgta cctggccaga cgcaaaatct
     6061 ttatgtcatt ggtggtgcag catgtgagaa gataggttct attactaaag aaaaatatac
     6121 tatgataaat ggcagtgata gatttgatac actttataaa gcattagatt ttattgatag
     6181 atagaaatat agatattaat accttgtact ttatatctat attttattta cagtttgcta
     6241 actatataat aagcaaaaaa tgatatatta gaaatatata agttgcttgc aaaaataaaa
     6301 ctaagatgga cataagttat aaatttaatt gtaagtataa atactacaaa atagaaaata
     6361 attagattga ataaattggt tgcatatgca gaattattta ttatatgaaa atataaagat
     6421 atgctatatt tttataactt actctattta taaagacagt atatggaaaa aattggttga
     6481 aaaaagataa aaccaatatt attataatag ataagagggt ataaatatga tttattgtca
     6541 tattttagaa attttattat ataaatataa tgttttaaaa atagaggagt tatatgatga
     6601 ataaagaatt agtttatggt gagattgatg aaaaaaatct tgatgtatca agaataagtc
     6661 atgaagaatt aaaggctcta ttcaatttaa tgaacgggaa acctgatagt atgcttagaa
     6721 tttttccaag agatgtaaat gtagaaataa aagatttata ttctttacat gaaaaaataa
     6781 aagaaaaatt acaacattat atagtagcag ctttaatagt gaatatatca gttaagtttg
     6841 ataacaatac tttcaaagag ttttctgatt ggatagaatt tcaaaattgt aattggtcta
     6901 gctcccacta tttagaaaat ataactataa agtatgattt ttttgttgat tttaaagaat
     6961 atggggttcc tcagagacat acattagtaa taagactatc ttcaggaatg aagcctcaac
     7021 agatattaca gctcatagta agtggtcaaa tagaagaaat agattctata gataaaagct
     7081 tatctcctgt tacttgcaaa gttgatttta ctaatcatct attaggtgat gaattaataa
     7141 atataataga agaatgggat aaatcaatac aaaaagtagt ttctgaatca ggaaaaacaa
     7201 tgaaatttat tagaaaatat agaaaaggta tttctgctgt tatagaaaat atcataccac
     7261 tttttggctt aattgttttg gtaagtatag ttaattttaa tttgtcgcat tttaatatag
     7321 atactttagt tcagttaagc ataagtcata tacaaaaatt aatcatgctt acattgcttt
     7381 ttagtcttat ttataaagta ataaaaaata ttggagaatt tttatcaaat aagtttttga
     7441 cagaaattta tgagtatggt gacactcaca tattcaacat aacgaatgga gataaaataa
     7501 aacgagagaa gttagatatc aaaaacaaaa aacatgaaaa aggaataaaa agtagcttat
     7561 cattaacgat tttaattaat gttatttgtg gaattgtggc aacttatata tgtactattt
     7621 tttaatattt gagtataatt ttaatctatt aagttcataa ttatataaaa atatatttta
     7681 aattgtattt atagctacaa aattaggggg tgtgttttat gataaaaaaa ataattgtat
     7741 tttttaaaga aagaaaacaa aaaaaagaat atttagaaga acaagctttc tcaaataagt
     7801 atatttctta tagtgactat aagtatttgt tagaacaaat taagtagtaa aattttatga
     7861 attttaaagt ataaaagcta tagcaatagt ttttatactt tttgttttta tgtctatagc
     7921 tgtaataata aaacgaacga aaattatcag actaaaacat aagacatgct ataattgtat
     7981 tagataaatg cttgaatata taccaaaagt actcttttta taagagtgct tatttttttg
     8041 aaattcatca acatataaac tatcaagaat attactcaac acaccttaaa atcgatttaa
     8101 attctttttc atacacaaag ttatatgata taataaaaaa gtaaatatgt aaccccaaca
     8161 catctttact aagtcaaaca ttattatata gagcattctt cattatggag agtgcttttt
     8221 catttctttg aataaccatg ttgattattt ataatatttt catttaattt atctttatct
     8281 atcaaattta atgcacaatt aatacaaatg ttagttttca tatgactttt cttatgaaaa
     8341 gtaatatact tattatcatc tttgtttatt cctttattac agtaatcaca taatatagtc
     8401 ttagtcatat ttttattcct ttcattttat attttccata attaagttta acatatttag
     8461 tatataccta tgtatatata tttaaaacaa tgttttttat tgcaatatag aatatattgt
     8521 ataaataaaa aaatatggag aatggatatt tgataattgt aaaattatgt tataattaaa
     8581 atgcaagaat atatcttgca agaggctaca atctaagggc gaaagttttc ggaaatcacc
     8641 ttcgaacgaa agggggtgag tatatgaaaa cacttatgtt tatacttgtt atagcgtatt
     8701 acattactat tgtaataaaa tctattacaa caatagtaaa gtctataaca agattattag
     8761 attctataag tagattaaaa gaatctttac ataaactaag aaatcgtcct gacggcaatc
     8821 aagacgattt caatgaataa atcattactt aaattatttt gaatttgaaa cttcgctcta
     8881 ggctaataga ttgtagtttc tttttttgtt tttgatacga atttacatct ttattatatc
     8941 gcattttaag aaaaaataaa actatgaata ttataaatct attttaatta attttttatc
     9001 atatcattat ttaaaagtag ataaaactaa aaagggttct ctagaaatca atgtctatct
     9061 aattatactt acttaccaat accatcactt ggtgtaaaca agactctact acattttctc
     9121 cgtactctaa tataaaatca tctaactgtg tatctaaaaa attacaataa tcatgttaac
     9181 tgctaatata agcagtcagt acatgtgtac atttatccat tttattttta aacatataaa
     9241 ttattatccc cctaaaaaac tttcataaaa acaaaaagaa caatatcttt ttggcaactg
     9301 gctgacataa ctcataagat atttatataa gtcttagtcc ctatagcttt gcgtcactaa
     9361 atttctctag ttttgccgat ttagttttat tctacaacta aaataataca ctagaattag
     9421 ttattattca acaagattgt ttgaaaatta aataaatttg tagatgtgaa taatattttt
     9481 tatatataaa aacaaactaa aacagtagaa tatatggtaa aaattaccta tagtttttgt
     9541 tctaaaaagc tcctattatt aatttaagtt agacgaatta aggaagtgac ataaatggag
     9601 cgacaagagg tagaaagtaa attacgcagg ggcgaattaa tgttgatgga gtacatgtga
     9661 agggaaaagt ctactctatc taaaaaagaa atagaagtag caatgaaaga aaagtataag
     9721 tggagaaaaa gcactacaga gattttactt ggaagattag taaaaatgag aatattaaaa
     9781 aagaagagaa ttggttttca attaaactat gaagtgttag taactaaaaa agaatattta
     9841 gatgtaataa aagaagaaaa agatgtaagt aaatatgata atttttttac acaagtattt
     9901 acaactatac ataaaaaaga agaaaagaca gaggaacaaa taaaagtatt tatagaaagt
     9961 atgcagaaac taggaaaaaa ataattttat atttttatat gttaatagaa agaaggggat
    10021 attatgctaa ataaaaaatt accagattca gagttcaaga ttatgaaata tatttggaat
    10081 actggttata aaactgtaat atcaaaagat gtggcagatg aaatggaaaa aatatataaa
    10141 tggaaacaaa ctacaacact aacactttta ttaaggttaa ctaaaagagg ttttttagct
    10201 tctcaaaaaa taggtaaaca cacacattat acaatattaa taaaagaaaa agaatactta
    10261 aaatcagaaa ctaaaaaact atttggaggt ttacataata atcctttatc acagttaata
    10321 tcaaaattac atgataaaga agaggtaagt attgataaaa tagatgcgct tgaagaatgg
    10381 ttaagaagtt tgcaagaaga tgaagaataa gaatatgcca aaaggtagca ctcaatagta
    10441 gctacctttt tgtggataaa gctgggtata aactgtggaa aagttatata aaaattgtgg
    10501 ataaaatgtt ttgctgacat ttaactgaca taaatattaa aacttgattt taaagtattg
    10561 aaatttcaat tattaatcat ataaaaatag aaaagttatt attttaataa agaa
//
LOCUS       DACXMZ010000138        10595 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.139, whole genome shotgun sequence.
ACCESSION   DACXMZ010000138 DACXMZ010000000
VERSION     DACXMZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10595
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.139"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(137..706)
                     /locus_tag="JD016_13185"
     CDS             complement(137..706)
                     /locus_tag="JD016_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAU5000584.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            909..1136
                     /locus_tag="JD016_13190"
     CDS             909..1136
                     /locus_tag="JD016_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAU5000585.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(1355..2098)
                     /locus_tag="JD016_13195"
     CDS             complement(1355..2098)
                     /locus_tag="JD016_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAU5000586.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            2266..3138
                     /locus_tag="JD016_13200"
     CDS             2266..3138
                     /locus_tag="JD016_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAU5000587.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            3138..3803
                     /locus_tag="JD016_13205"
     CDS             3138..3803
                     /locus_tag="JD016_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAU5000588.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            3824..4681
                     /locus_tag="JD016_13210"
     CDS             3824..4681
                     /locus_tag="JD016_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAU5000589.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(4837..7383)
                     /locus_tag="JD016_13215"
     CDS             complement(4837..7383)
                     /locus_tag="JD016_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAU5000590.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(7383..8069)
                     /locus_tag="JD016_13220"
     CDS             complement(7383..8069)
                     /locus_tag="JD016_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000591.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(8152..9141)
                     /locus_tag="JD016_13225"
     CDS             complement(8152..9141)
                     /locus_tag="JD016_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU5000592.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(9128..9814)
                     /locus_tag="JD016_13230"
     CDS             complement(9128..9814)
                     /locus_tag="JD016_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000593.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(10314..>10595)
                     /locus_tag="JD016_13235"
     CDS             complement(10314..>10595)
                     /locus_tag="JD016_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000594.1"
                     /translation="KSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKMRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         3735 a         1749 c         1542 g         3569 t
ORIGIN      
        1 aataaaatta tataattaaa aatactctat aaaagctaaa aagtttttct attcgctaca
       61 tctaaaaata attagataga ggtataaatg tatttagttt atttatacct ctagtctttt
      121 ataaattata tctctactaa attgattttt tagcacgaat aagtgtgctc ttggagatac
      181 cagtcatctt cacaacctct ctatatggct tagaatttaa taaccccaat gcatattgta
      241 tctgttgatt gctgtatttc tttggcctac catctttaaa gtcctttttc tttttagcaa
      301 attcttttcc taattgagta cgttctatta tcatatttct ctcaagttct gcaactgcaa
      361 gtaatgtagt caaaaagaat ttacccattg aagtattttc taaaaggcct atatttaaaa
      421 tatgaacact tacttcttta gcaaataaac cttctattag ttgtattcct tctgatgtac
      481 ttctagctag tctatccagc ttagttgcaa caagtaagtc tccctttttt agagatgata
      541 taattttttc aaatttaggc ctgtttgtag tagtgcctgt ataagtttcc tttattattt
      601 tagcatcatt atatttattc aaaatttctt gctgttgctg atacaaactg ttgttgtctg
      661 tttgattttt tgtacttaca cggcaatatc catatataat aatcatcact tcctaacata
      721 aagattttgt acagaagtta cccagaagct tgtttttact tatctttatc cattttacta
      781 aaacgatttt tgggtgtcaa tactgttaac ttttgacacc ataataattc ctttataatt
      841 tgtttgacta tgtcaaaaat taccaatata atgaaattaa tgagtttata caaattggag
      901 gaaataatat gaatagtctg ttaaaagaag caatagatga attaaaaaac atccattgcg
      961 gggaagtttt tttattaaaa gaattgttca aaggctatga atggaacagg ttggagatga
     1021 atatcagatt gaaattagga aaactttttt tgtatgaagt ggtaactaat cccaatttaa
     1081 atgtagaggt catcaataaa aattctgctt atcagcaaat gtataaaaaa aaatagtgac
     1141 ttaaatgtat ataatagtat aatcttctta ttgattcaaa agttaaaact tttaatacaa
     1201 aacaatcatt ctattttaac atttatcaat atgtctaaat ttatataatt aacacatacg
     1261 aatagtattg tttaaatcac attatttaag atagataaat cttaacccta ctccagcata
     1321 aatactaaaa tagggcttag aattatctat atttttatcc cataaaatgc ttagctcttc
     1381 ctcttgattg aacatatata tctggggaaa tacaatagaa gcgtaaattt ataaaatttc
     1441 caaagtcaga aaacttcagt tcaaacttag gtataccaaa actttcaggt gctatttcgt
     1501 caacgccaaa atagatgata attccattat ctgtaagata aaatgcttgg tcttcaggta
     1561 taactaactc aacattagaa taatacattt ctctattttc atttatttga gatttaatgt
     1621 aatcagtaat tatattaata taatcaactc catctctaaa aatgtcatta agtaaaagtt
     1681 cttttccagt tgtcaaatct atattgtaat tattcaatgt attgtattta ggcccatagt
     1741 tattagcaaa ccccataagg tttaatatca tactcagtac ataattttta ttaaaagtaa
     1801 ctgcgtaact ggtagatacc caatagcttt gttttggaag tgaattttga gaagctgcgt
     1861 tattatattc tcgttcttct tcctgtaagc catttttaaa attatttaca gtcattctta
     1921 cagcattgtt tatgttattt aaaataactg ggtttacata cgtggattga tttctaatat
     1981 tgtatataac aggatacttt acatcatatg aaaaatactt acttctttca aaatcttcat
     2041 taaattgtac atataatata ttatatgtac aggtatttgt agattgataa ttttccatta
     2101 aaatcctccc ccttttataa aaaaatcaga tacaatataa tatgcttaaa acagtaataa
     2161 tgttaatggt aaatgtaaag aatagaaagt atctaaaaca gcaaaaaatg actaattttg
     2221 tggtataata cataggattg agagttattt tataggagga aaaatatgtt attattagca
     2281 gataaatgga aagattatga acttatagat atgggaaatg gagaaaaatt agaacgttgg
     2341 ggaagttatg tacttagaag accagaccca caggttgtat ggccaatgga atctgaatgg
     2401 gctttatgga aaaaccctca tggacattat cacagaagca ataaaggtgg aggtcaatgg
     2461 gaacacaaaa aaagatatcc agaacaatgg actataaatt ataaaaactt aaagtttcac
     2521 attaaaccaa ctggctttaa acatactgga ttattcccag agcaagcagc taactgggat
     2581 tggatgatag agaaaataaa gaaagctaac agacctataa aagtgttgaa tctttttgca
     2641 tatacaggtg gagctactgt agcatgtgca tctgctggtg cagaagtatg tcatgttgat
     2701 gctgcaaaag gtatggttaa ttgggcaaaa gaaaatatag aattatcagg tcttaaaaat
     2761 caaacagtaa gatttatagt ggatgatgtt gtaaagtttg ttgaaagaga aataagaaga
     2821 ggtaaaaaat atgatgcaat aataatggac cctccttctt atggaagagg tcctaaagga
     2881 gaagtatggc aaatagaaga aaagttatac agttttgtag atttatgtat gggagtttta
     2941 tcagataacc ctttattttt cttaattaat tcttatacaa caggattttc tccaatagtt
     3001 cttgaaaatg tattgtctac aacaataggt aaaaagacaa atacaggaga tatatacggt
     3061 ggggaattag gaataccagc ttccagagat ggtaagatac ttccttgtgg tatatttgga
     3121 agatgggaga aaaaataatg ataaaagtca tatatgaaga taaccattta ttagttgtag
     3181 aaaaaccagt taacatattg tctcaaggag ataatactaa tgataaagat atggtaaatc
     3241 ttttaaaaga ctatattaaa aaaaagtata acaaacctgg aaatgtatat attggtttaa
     3301 tacatagatt ggatagacca gtaggtggag ttatggtttt tgcaaaaaca tcaaaagcag
     3361 catctagatt gtctgagcag gtaagaagta agactttcaa aaaaacttat ctagctgttg
     3421 ttcatggtaa gataaaatct aactcagata tactaaaaga ttttttatat aaaaataaaa
     3481 aaactaatat ggtaactgta gtgaaaaaaa gccataaaga tgccaaagaa gctgaactta
     3541 actataatgt attagatttt aaagaaaatc taagtttagt tcaagttaat ttaaagactg
     3601 gtcgttctca tcaaataaga gttcaatttg caagcagaaa acatccatta tacggagacc
     3661 aaagatatgg caaagaaata aataaaattg gacaacaaat atctctttgg tccaataaga
     3721 tagaaatttg ccatcctact acaaaagaaa aaatggaatt tacatgtcat ccacctgaca
     3781 aatatccatg gaatatattt taaatctcaa atgaggtgat tatatgagta aaaaattttc
     3841 aataggagaa atgtctaaac ttcataaaat aagcatacag acactaaggt actatgacca
     3901 aatagattta tttaaacctg ttcatgttga ccaagaaagc aattacagat attactcaat
     3961 tgaccaattt tcacaattag atattataaa atatttaaaa tatttaggta tgcctttaaa
     4021 agaaattaag tcaaagcttg atgaaaataa tgaagaaatt ctaaaattac taacaaataa
     4081 aattaatata gtggatgaaa aaattaaaga attagagctt ataaagaagg tactaagcac
     4141 aaagaaagaa actatggaaa aaggtatgaa agtagatgaa gttggtaaaa tagtgagaaa
     4201 acatattcca cgaagaagta tcttatctat agagtatgaa gaaggattaa gtagtgaaga
     4261 aactatggaa ctttccagaa gacaaatagc aaatatatta gaagataata tatctgtgtt
     4321 ctatggtgga gtaagtggac ttgtatctat agataaattt ttaaaagaag ataaagttat
     4381 ttatgacaat agttttgtta ctgttgaaag agacttattt gatataaaat cacaaaaaca
     4441 tataacagaa atttcatctg gtgattttat ttgtatgaca tataaagggc cttacaaaga
     4501 aaattatacc gcttttaaga aactggttga ttatgttgaa tataaaagaa tacctattga
     4561 aaattatata tatgaaatac ctataataga ccctctgtct acaaacaacg aagaggaact
     4621 tctaacagaa attcaaatac aagttaaaaa gagagaaatt gacttaagat gtctagtgta
     4681 gtaatctagt ttacttacac atcttaatat agttgtctat tatcaacata tgatttttct
     4741 agatagtgat tcctaacagt gagattttca aaaaatagga ggaaatatgg ttaatgctaa
     4801 ttccctccta tttttaatta atccatatca ttttaattaa gccatgtcat cacgaagtgc
     4861 atcaataata ttttcttttt ttatcttatt aatagtatac aacattgtaa taaatactat
     4921 aaataaaaca ctaaatatac taattccaat actaaaccat ggaatcacaa aatcaatatc
     4981 atctgtaacc attactttat gaattagcca tgaagaaatt actgctatag gaagtccaaa
     5041 gaacaatgct ctcatgccat aaaaggcaca ctcaaaattc atcatctttt ggaaatcatg
     5101 ctcagacatt cctacagagc gaagcatggc aagttctcta cgacgtaact taatattagt
     5161 tgaaattgta ttaaacacat tggcaatcga aatcagagaa atcataatta tgaaagtata
     5221 agcaaacaca ttggcaataa aaatgtaatt acggttctca tccatcattc catacatatt
     5281 gtacagattg tactcattcg taactccctc actctcaata atttgctcca tttctgcaac
     5341 tgactgagta ggatttttcg attgaaagct taaactttca attcctgtag aaacaccagg
     5401 aatctcaaac ttatctttgg ctgaataagg agccaatact ctgaagcttg ctttcttcac
     5461 atctgaactt ctgattcctg gaggagaatc aggtggcact actttgacaa atgaaatatt
     5521 cacattttgt ctctcaggta tctccaactt gccattagtt tcaggagtga ggctaacatt
     5581 catagaagaa ctgttgaaaa tatcaataat attattatcc tttgttacca tagaaacagc
     5641 aatcagtttt gcattttgtc cagtatattc ttctgctggt aatccaagac ttttaataat
     5701 attcagataa gttctatcat caagaaattg catatctact ggtaattcaa gcatttcact
     5761 catagaatgt gaaccctcat agttcaaata atcgtctgta agctcactta ctttggcaac
     5821 acatgaatag gtcataagtt tctgatacaa accttcgtaa acaccatttg cagtttttag
     5881 cttatcgtaa agcttcaaca tttcgttgtc tttcatacct tgtgtagaaa aaataatatc
     5941 ctggtcagta acctctgacc tctgatttga catctgtttt aggtctgcta ctaaagcact
     6001 tgtagatata aataaaacta tgcttaaaac aagcgacaat acaatacttc gataacgttt
     6061 cttgtttctt ttaaagtttt tcatagcaag aactccctca agaccataaa ctcgctgtgt
     6121 tagttttgat gtcttcacag atttagattc aatttttacc tcatttgtct gacgaataca
     6181 ctccatcact ggtgtgttgg cagcctttct tgctggtatg taagcagaaa ttaagattgt
     6241 aaccatactt accactgctg caacgatgat tgcagggata gacaatgtta atgtcaacgg
     6301 aacgtttaca tagagaatat ttgcaaagtt tttagctatg acagaaatca caagtccaat
     6361 actgactata ccgattgcaa caccaattgg tataccaatc gcaccaatgc aaattccttc
     6421 aaatagcacc gaatttcgta actgttttgc tgtagctcca acagatgaaa gtattccaaa
     6481 ttgatgtgtt cgctcgttca atgacatgtt aaatgaatta tatattagaa aaattgagcc
     6541 tagcatgatt atggcaagca caatgccacc aaccgagtac aaaaacatat tgaacaaatt
     6601 gttatcagaa acacctatga agcgcaacac attgtcattg aaaatggaag catggtcttt
     6661 agtgctactt atgtaagagc gaatctgatg tggatttttc aatgtgacaa aaaggctaag
     6721 actgtcagtc ttgacttgtt tatcttcttt ggttatcaag gtatatcctg gtgcagaaga
     6781 ttcttcaaat acaggctttc tacagatacc cacaactgta taagttttct tatcctgtgg
     6841 cacaaaaact tcactcccag cagtgtatgg gtcactctga ccaagctttt cattttcact
     6901 cattcgtttc cctacatcca aagaaatagt atcacctact gcaaatttga caccaccatc
     6961 tgtcgcaact ttacttgaaa caagaatctc tctactattt tcaggtaatc ttccagaaat
     7021 taaagttata ggcaaagtat caaaagcttc tttgctaaat ccagcaataa aaagatatgg
     7081 tttatctgga tttctgttac cttcaagtgt tgcatagcca atattttcaa atgttgcagt
     7141 atttgcaact tcatcattga ctctttgttc ctgtacaaaa gaagaatcca catccaaaaa
     7201 ctggacatgc caatcaccat attttataat agctccattt gtcatatagt tcatcaaaga
     7261 aactccaaag gtagtaactg ctgtaatcat agcagctgac agaacaactc ctataattgt
     7321 tacaagagtt cgagtgcgat tctttttcaa tccttgcaga gcaactttgt taaaaatgtt
     7381 cattaacgat tcaccttcct ctcatcacgc actacttttc catctgaaat acagataatt
     7441 ctatctgcct gtaaagcaat gttttcatca tgtgtgacaa taatgagagt ttggtgatat
     7501 ttactatggc tctctttcag taactggata atctcttgtc catttttact gtctaagcta
     7561 cctgtaggct catcagcaag cataactgct ggtgcattca tcaatgcacg accaatggca
     7621 acacgttgct gttggccacc agaaagttga tttggtaaat gtgtttttcg ttcttttaaa
     7681 ccaagtaatt ctaacaagtc atttaaccgt tcttgattga ccttacgctt atccataagt
     7741 ataggcaatg tgatattttc tactacattt aatgtgggta tcagattgtg aaactgataa
     7801 atcaatccaa cttgtcgcct acgaaaaata gcaagttttt cattgctttg gccatataca
     7861 tcttgcccct ccagatatat ctttccactt gttggtacgt ctactcctgc aatggaatgt
     7921 agtagtgtag atttaccaga accagaagaa ccaatgattg cagtaaactc tcctttttca
     7981 atagtaagtg agacatggtc aagtgcagta accttatttt cattcttacc ataaacctta
     8041 cataaatctt caatctttaa aaacgccatt atataagtct cctttcatat tttataaata
     8101 tatcatagga ctttcaactt acatttaagt gacatttaaa tgagagattc gtcactttgg
     8161 aaaacgaata acaaatattg caccaccttg tgggtgattt tgtgcagtaa ttgttcctcc
     8221 ttgtctcata ataatcgtct tacaaagtgc caatcctatt ccataaccag ctgtgcttga
     8281 atttttccca cgataaaatc tgttaaatag acatggtaag tcttcttttt taaagcctgc
     8341 tccgttgtca tgaatagtaa gttgagtaaa caaaaagttg tcctcacaaa tgatatcaat
     8401 ttttccatta tcacccacac tctccatgca gtttttaaat atattttgaa ttgcttctga
     8461 aagccaattt aaatcaccca aaatccttac tccttttggt atatcaatat gtaaagtgat
     8521 attgtgcagt tccataggaa tcaaaagttg atgaagtgca gaactaatca aattattcac
     8581 atcaatttgt tctttttgaa agactacaat acctgcatct aaacgtgata attttaaaag
     8641 ggaagtaatc agccagtcca ttcgtataag taattcctct gtttctcgta tcaactcttt
     8701 tctctcatct tcatcagaag tattctctaa cagtgacaaa ataagagtta ctgatgtaag
     8761 aggagtgcga agctggtggg ctatgtcagc tagtgagtca gcaaggtgtt ctttttcttt
     8821 tttcagtgcg taattttgct ctctaatacg taacgtcatt ttagttattt cactttgtag
     8881 aatagaaagc tcaccctcat ctgattcagc aatatacaga tggttagcat tatgaagcac
     8941 aagattaatt tgttctgaaa tctgtgcaat acttttgtat cgatttttag taaacacaaa
     9001 aaatgctatt ccaaaggtag tggcagaaac aatagaaagt attccagtca taatactgat
     9061 tgcaaatccc agggctacag tgagaatagt aattaataag aataaaatag caaattttcg
     9121 aaactctcta tttcgaaaca taacttcccc ccaatctata ccctgtccca cgaacagtca
     9181 aaattatttg tggacttgaa gggtcatctt ctatcttctc tcgcaagcgt ttgatgtaca
     9241 cagtcaatgt gttgtcatta acaaactcac ctgccgcatc ccacaattca tcaagtaatc
     9301 ttaccctcgt aataatactt ttaggattgc taataaacac caataacaaa cggtattcta
     9361 aagctgaaag aaaaacctca ttgccattct ttttcacaac accggttgct gtatctacat
     9421 aaagcccaca aatttcaaaa gttgacccaa aacgcccact ttttcgtagt gctgttctaa
     9481 ttcgcgcaat cagttcacgt ggacgaaaag gtttagtaat ataatcatct gcacccatat
     9541 tcagtccagt aacaacactt gattcatcac cggaagctgt caaaaagata acaggaatgt
     9601 cttgcttttc tttgatttct gtgcaaatcg taaacccatt tccatcaggt aaagaaatat
     9661 caataagtgc caaatcaaat ttattcttag caatctctac aagtgcttca cttcttgtgg
     9721 atacatgggt tactataaat ccttctgaat taagcaaaag aataagattt ttggcaattg
     9781 ctttatcatc ctcaactaaa aatatccgtt tcatttactt tcatcctcct ttattttatt
     9841 agttttatat attccagcag agcaatagta cactactatt ttacttatca ttacacaatt
     9901 ttcagtagtt tcttttgttc ttatataatc catgcataat acctccattg atttattata
     9961 tttcttataa cataaataca caagataaga caaaaaacat aatgtaagtg caagtttttt
    10021 aactcactaa cttataaaca tacactccta ctaacactac tttcttgatg acacatctta
    10081 caagcttata ttcatacatc aatgaaaaat taaatttatc catttattaa aataaaaggc
    10141 aatagcttct atagctaatg ccctttatct taaaatgatt taaatactat acaattttta
    10201 taaatggcta taaataaact cgtatagaat ataaaattta tctacagtct tttgttatac
    10261 aaaatttata acctcttata gaacaaattt accactttgc aaacttatca cttttactca
    10321 gctatattat aaacttttaa tttatctttg tttaaatgtt tcttcttaac ttctactaaa
    10381 gcttttagcg tatccaaaga tgtcattatt tcagtagaaa attcaatggc tgttcttctc
    10441 atcttaaatc catctcttgt agagtcattt cctttagttg gtgtatttat tactatatca
    10501 acttgtttat ttcttatagc atctaatatg tttggtctag gctcttcaat tctatttact
    10561 atatcagcct ctattccatt ttctcttaaa ctctt
//
LOCUS       DACXMZ010000139        10568 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.140, whole genome shotgun sequence.
ACCESSION   DACXMZ010000139 DACXMZ010000000
VERSION     DACXMZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10568
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.140"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            267..3170
                     /locus_tag="JD016_13240"
     CDS             267..3170
                     /locus_tag="JD016_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU5000595.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3203..3649
                     /locus_tag="JD016_13245"
     CDS             3203..3649
                     /locus_tag="JD016_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAU5000596.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3772..4074
                     /locus_tag="JD016_13250"
     CDS             3772..4074
                     /locus_tag="JD016_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU5000597.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4255..5661
                     /locus_tag="JD016_13255"
     CDS             4255..5661
                     /locus_tag="JD016_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAU5000598.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            6005..7063
                     /locus_tag="JD016_13260"
     CDS             6005..7063
                     /locus_tag="JD016_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAU5000599.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMEKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7162..7848
                     /gene="araD"
                     /locus_tag="JD016_13265"
     CDS             7162..7848
                     /gene="araD"
                     /locus_tag="JD016_13265"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAU5000600.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7866..8513
                     /locus_tag="JD016_13270"
     CDS             7866..8513
                     /locus_tag="JD016_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HAU5000601.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8686..10053)
                     /locus_tag="JD016_13275"
     CDS             complement(8686..10053)
                     /locus_tag="JD016_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HAU5000602.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10243..10331)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
BASE COUNT         4083 a         1253 c         1858 g         3374 t
ORIGIN      
        1 tactattata tatgattgag caagtttttc aatcataaat ttataaagta ttttgcatta
       61 aaatttaaac aaagagttaa attatttaag attatgaata actaatacat ttagtaaatt
      121 actaaatata caaaatgtaa atacttatca aaatgtataa atacttatga catctagtta
      181 cttacagggt ttaagtagaa aaaaatatca aagtaagtat ggcatgatag ttgcttatat
      241 atttatcaag aaaggagtta ttaagcatgg agttgagaga taaacttgta aacatcataa
      301 atacagaaaa taaaaaagat ccattaacag atgttcaaat agcaaagttt ttaagtacaa
      361 ctagagagaa cataactaat ttgagaaaag aacttaatat aagtaattca aggcaaagaa
      421 gatatcctta tttaaaatca gcaatttcgg caatattaca aaagaataag aatatttcta
      481 tatcagaaat tacaagggaa ttgatgacag aaggctttaa tatatctaga agagttgtgg
      541 aagaactttt acctaaagaa agtttagaaa tggatgtagt agaggaatct gaagaagaat
      601 cgaatgaccc atttgaaact ctgataggta ataaagggag tcttagaaat gctgtagagc
      661 aggctaaatc tgctatattg tatccaccaa aagggttacc aacgttgatt attggggaaa
      721 gtggagttgg taagagttta ttttctagac atatgtatga gtttgcaaag caaaaaaaga
      781 tagtaaaaga gagctccaat tttgttgtat ttaactgtgc tgattatagt gacaatccac
      841 aattattatt atctttgtta tttggatata aaaaaggtgc atttacaggt gctgaatatg
      901 atacaccagg tcttgtagaa gaagctgatg aaggagtttt atttttggat gaaatacata
      961 gattgcctcc aaaaggtcaa gaaatactat tttctatttt agatagggga aagtttagga
     1021 gattaggtga aactaatgct gaaagaaaag taagtattat gttgattggg gcaactactg
     1081 aaaatgtaga gactaattta ttattaacat tcagaagaag gataccaatg ctaataacct
     1141 tgccaccatt acatgataga ttactaaaag agaaaataga ccttatatac aatatctttc
     1201 aacaagagtg taatagaata aatgcaaaaa tatttgtaga taaaaatgtg attgaaatac
     1261 ttgcactaaa aaagtttagt ggaaatatag gacaattaca aaatatgatt caggttcttt
     1321 gtgcaagagc ttttatgaag tttataaatt ccaaagatga agatagtgaa tcgatagtag
     1381 tagtagatat aaatgaagtt ttaaagctta aagattcttt taaagatgct gcctttcaag
     1441 agattgaata tacagagata agaaaatatt tgaaaaatgc tatatttata ccatttaatt
     1501 tagaagaatc atcagtaaaa ggaagtcttt taagtgatga atatatatta ccagaggaca
     1561 tatacaaaac aattgaaaag aaatattatg acctaaaatc cctagacatt gaagatatag
     1621 atatagagaa tattttatgg acttttattt taaatagatt tgctgactta aagtttaatc
     1681 tcgatagtaa tgaagaaatt ttttcaatga gtgatttaaa tagttttgta agtgatagtt
     1741 tagttaaact ggtaaaagaa cttatgaatg atatagttga aaaaaatatg aatttagaag
     1801 taaataaaaa tatgttcaaa tatctagcaa tacacttaga agaagctata aaaagaatta
     1861 agctaaatca aaagataata aatgtaaatt tagaaaaaat aaagattgac ttttcaaaag
     1921 aatatgaaat tagtaaaagt tttgcaataa aaattgagga atcaattggt attaagattt
     1981 cagatgatga aattggattt ataacgctct acataaagtc tgctctaaaa aatgaagtga
     2041 aaaaagataa agtagggtta ataataattt ctcatggacg tatagctaca gagactgtaa
     2101 atgtagttaa agaactttta ggtgtaaagt ttcctgtggc tatagatatg cctttagatg
     2161 aaaaaccaat aaatatatac aataaggcag tagaactgtc aaaaataata gaccaaggga
     2221 aaggcatatt gtttttggtg gatattggtt cacttacaaa cataggtcaa atagtaaata
     2281 agagaaccaa tataagtact aaaactattg atagagtaga cctcctaatg gcactagaag
     2341 caactagaaa agtttctatg ggagaggaag aacttgatga aatatttttt tctttgctaa
     2401 aagataggat gggatataac tatgaattag aaaaacacat agataaatca aatgcaatta
     2461 taacattatg tcttactgga gaaggaactg ctaagtatat aagtaaaacg ttggaagaaa
     2521 agtatgataa cactaaatgt tatcaaatga gtgcattgga tgaaaattta ttttgtaaaa
     2581 ttgaaaatct aaaagagaca aacaatatat tagctattgt tgggacaata aaccctaaga
     2641 taccaggtat aaattttata ccctataata aagaactttt caaaaatttg gatatctatc
     2701 tatcaaaatc aaaacaacaa gatgacagac cagttaaatc gtatgaaaga atgcttgatg
     2761 aggatttagt gatttttgaa ccagatatat attttaaaaa ggatttatta gaatatgtat
     2821 gttcaattct tataaataaa gaatatgtag aaaaagatta cttagattct gttttacata
     2881 gagaggaaat gcttccaaca tattctaaag gagctatagg tgtgcctcat ggagattctt
     2941 ctactgtaaa tagtacaagg tttgtttttg ttaaactaaa gaatcctatt gattggggtg
     3001 taggtaatgt aaatttcata tttatgccag tttttcaggc taatgataaa gagattgtta
     3061 aaaatgttct tgagatacta aaagatgcag aatttatgaa caatgccaat aagtgttttg
     3121 ataaagatgc ttttagaagg attatatttg ataaatttaa acatctgtag ccatagcaaa
     3181 aaaattgaga ggagttagag taatgaatgt agatttaata tcattgggtt tagaatctaa
     3241 tgattatgaa agtgttattg aagaattagg aagtataatg tgtaaaaaag aatatgtaaa
     3301 agaaacatac ataaatgctg tcttggaaag agaaaggaca ttaccaacag gtctagatat
     3361 tggagacatg tgtgttgcta ttccacatac agattcaaag catgtaaatg agtctaatgt
     3421 agctgtaggg atattgaaaa atcctgtaaa gtttaattca atgatagacc caaaagatag
     3481 acttgatgta gaacttgtat ttttattagc agtaaagaac ccagactcac aagtaaaact
     3541 gttaaaagat ttaatgtcag tttttcaaaa tataaagtta ttaaaaaata taaaaaacgc
     3601 atcaacaaaa gaagaagtag ctaaattatt ggattttata gaaatttaat gtaggagaat
     3661 tttataaata aaaaattaaa atttaaacaa tcaaagtaag tattattttt ttaataggtt
     3721 taatcttata taaaataaaa atttagagtg aaaaattagg aggaaattat tatgaaacaa
     3781 tttaatgtat tatcagtttg tggttcaggg acagttacat catctatggt agcaggtaaa
     3841 ttaaaagaag tactaggtaa taaggggatt tctattacta caacagaagc tagaccaaca
     3901 gaagcactaa atttagcaca atcaggaaga tttgacttta taacatttac gagtccactt
     3961 caaacaggcg attatggaat accaactata aatgcatttg cttgcctaac tggtataggg
     4021 gaagaggcat tctttgaaga agttttgact gttattgaag ggatagataa gtaagttttg
     4081 gagacgtgaa tttatagtat ttaaatcaat gatttagata ctataataag ctaatattaa
     4141 ctaaatattt gtggaaaaaa cattttatat gttgtcataa attaaaaaat tataaaaata
     4201 gtcacttaag tattaaatta agcttataaa tatttataag taggagggaa aaagatgtta
     4261 gaatttatga aatctataat cgatacattt ggttctgcaa ttatagttcc aattataata
     4321 ttcataatcg caaagatatt taaagtaact actaagaaat cgtttctttc tgctgtgtat
     4381 gcaggagttg ctttacaagg ttttacactg attttaaatt catttacacc agtaataaca
     4441 ccagttatca ataggatggt tgagagtaca ggagtaaacc taccagtttt tgatgttggt
     4501 tggcaagcaa catcattggt tgcattttca acaagtgcag gtatgatata tttaggtctt
     4561 ggtatattat tacaaacaat tttatttttg ataaaatgga cagatgtgtt tcaaccatca
     4621 gatttatgga ataactattc ttatatggta tggggagcaa tggttatagg agtaacagga
     4681 aattttcctt taggtatagc atgtatggtt ttattaaacc tatatagttt attaatatca
     4741 gagttagttg caaagagatg gtctagttac tatagatatc ctaattgtac aataatagct
     4801 atgcataatg ttgaagcttc tgtgtttgca gtatttgcag accctatata caataaattg
     4861 ggattaaata aaataaaatt aaatccaaaa gaattagaga agaaattagg gtttttaggt
     4921 gaaccaatta ctttaggtct ttttttaggt atgtttatag gtatattagg taatatgacg
     4981 agaataaata ctatggaagc ttggggagaa ataatgaaag taggtatatc tacaagtgca
     5041 gtgatggcaa ttttccctaa agtagcaagt atgtttgctc aagcatttgc acctataaca
     5101 gaagcagcta gaaagattat gcaaaaagct ggaaaccgtg agtggtacat agcagtaaat
     5161 gatgctgttg gatatggtga acctgcaact ttaatatcag gtcttatatt aattccaata
     5221 atgcttgtaa tagctatggt acttcctggt aataaggtat taccagttgt ggatttattg
     5281 gcaattccat atatggttca aggtttagtt gccatacata atggtaatat accaaaagta
     5341 ttagtttcag gtataatttg gtttggatta ggattatatg tttgtacatc tacagcacca
     5401 ttatttacag atatggctac aaatataggt gtagctatac ctgcaggagc aatgttaata
     5461 acaagcttta atatactagg aaaacctttg atgggattag ttttctttgc atttctaagt
     5521 gcaaatccaa tttatatagg tctttcagtt gtaatttact ttgttctatg ggcattgttt
     5581 agaaagaaca aaacaagtat tcttgattat ttagaaaaac aagcactcaa aaatgtagaa
     5641 gaagagcctg tagcagttta agtttataaa aatgaaataa aaataattaa aggagtttat
     5701 aattgatttt atataagtat cagataatac ctgagaaata tttaaatgaa atatagactt
     5761 ctttttattt gataatattt gtaattataa tgcattgtat ttttatagtt gagttttctg
     5821 tataacctgc tatttaaaac atactctttt atatttatac agtaatgtgg tttaatttta
     5881 taaaatgtaa tctctactgg taaaagactt tgtatataag gcatttgcct gaataaacca
     5941 tttatctttt gtatggcata gatattgcta tatagaagta tatagtatga aaggagatat
     6001 ttatatggga gataaaatga gagcatcagt attgtataat gtaggtgatg tcagatatga
     6061 aatggtagat ataccagaaa taacagatac gcaagtgctt gtaaatgtaa aatatgttgg
     6121 gatatgtggt tcagatttac ctaggtctat ggtaagtggt ttgagtggaa atacaaaata
     6181 tccattgatt ttagggcatg aattttctgg tgaagttgtt aaaattggag aaaaagtaaa
     6241 gcatattaat gtaggagata gagtagctgt tgcaccatta gtaccttgtg gaaaatgtga
     6301 ttattgtaat gaaggtaatt ttggcttgtg tgatgattac aatataattg gaacaagagt
     6361 aaatggagcg tttgcagaat atgtaagagt tccagaagaa catattttaa aactaccaga
     6421 tacattggat tatgaaactg ctgccggaat tgaaccagca actatagctt atcatgggat
     6481 ttcaaagtct aatattagag taggagatag tgtagttgtg ttaggatgtg gtccaatagg
     6541 tcagtttgtt atacaatggg caaaggtgtt tggtgcatct aagattatag cagttgatat
     6601 ttttgatgaa aaattagaac tttctaaact tctaggagca aactatatac ttaactcaaa
     6661 agaagtaaat gtaatcaaag aaataaaaaa aataacaaat ggtggtgctg atgttgtaat
     6721 agaaactgct ggtagccgat ttacacaaga acaatctcta tttgtagcaa aaaagagagg
     6781 aaatatagtg tttgtaggta tatcacacac agaattacca cttagtgcag acgctactga
     6841 gtgtatatta cgtggagaac ttaccttgaa aggttcttgg aattcatata caagccctta
     6901 tcctggaaga gcatggactg caacattgga ttttatggaa aaaggtgaca ttatttttaa
     6961 acccatgatt tcagataaaa taggtcttaa tgaagtgggt gactttttaa gtaaaatgag
     7021 caagagagaa attaatttca ataaaatcct agtagaaata taaaaattta ataaaaaatt
     7081 ctaaaagcaa ttaaatttta taactatgac attaaataaa ataaaaataa ttttcttaaa
     7141 taaaatatag gaggaagaaa agtgttagaa aatttaaaag aagaagtatt aaaagcaaac
     7201 ttagaattac ctaagaaaaa gctaattact tatacttggg gaaatgtaag tgggatagat
     7261 agagaaaaag gattagtggt gataaagcca agtggtgtag agtatagtaa tatgactgta
     7321 gaagatatgg ttgtagttga tttagaggga aatatagttg atggcaaatt aagaccttct
     7381 tcagataccc cgactcatct agttttatat aaagagtttg aagaattggg gggaatagta
     7441 catacacatt caagttgggc aacaatttgg gctcaaatag gaaaatctat accatcttgt
     7501 ggtactactc atgcagatta tttttatgga agtataccat gtaccagaaa aatgactaga
     7561 gaagaaattt gcaatgaata cgaaaaggaa actggaaatg taatcattga agaatttatg
     7621 ggaagaaatc caattcattg tccaggaata attgtaaatg accacggacc ttttacatgg
     7681 ggaaaagatg caaatgaggc tgtacacaat gcagtagttt tagaagaagt cgcaaagatg
     7741 gcttattata cagagttaat gagtcctgat aatattatgg ataaggttct tatgaataag
     7801 catttttcaa gaaaacatgg aaaaaatgca tactatggtc aaaaatgatt atctggaggt
     7861 gaattatgct agactcttta aaaggtaaag ttgtggaaat agcaattaaa gcagagcatt
     7921 atggtttatg taaagaaaag actggtaatt ttagtattag agataaggat actggatata
     7981 tacttataac cccatcaggt gtaggaagag aagaattaaa agttgaacat atttgtgtat
     8041 tggatttgga tggaaatgta atagaagttg agaaaggtat taagccaagt agtgaggtat
     8101 tgatgcatat tgaaatatac aaaacaagag aaaatataaa tgccatatca catactcatt
     8161 ctttatatgc aacagcattt tcagtagcaa agaaaccaat tataccaatt gtatatgaat
     8221 cagttaatta tggcggttat gtagatattg cttcatacga aagaccagga actttaaaat
     8281 tagctaagag tgtttcaaaa ttactgatag atacagatgc atgcttatta gaaagacatg
     8341 ggttgacaac tgtaggtaat gatttagacg aagcattatt aaaatcaaga tatgtggaag
     8401 aggtagcaga aatatactat aggtctttag tattaaatca atttaaagaa cttgaagtag
     8461 tagatgttga tgagtttaaa tcttggaaat acccagaaga aataaagttt taatctaaat
     8521 aattttcatt gaagtggtta attaaaatat atgtattatt tatatcaatt actatgtttt
     8581 attatgttaa ttaccgtatt ttgcaaatat atttattaca ttttttcaaa aaaatataga
     8641 atccgaaagc catataaaat tgacttggga ttctatactt tttatttatg catttttctt
     8701 tatctgattt tctggagaat ttatatgttc aaaatatttt ttagtttcag atactattat
     8761 aggtgataat gcaagtaaag ctataaggtt aggtattgcc ataagcccat ttacaatatc
     8821 agcaattatc caaacaactt ctaactttaa gaaagcacct aataacacca tagccacaaa
     8881 tacagttcta tacccattca tgccttttac accaaacaga aattcaaaac atctttctcc
     8941 ataataactc catcccaaaa ttgtagtaaa tgcaaataat gttaaactaa ctgtaagtaa
     9001 tattggtcca aatgtaggaa ttgctccgtt aaaagctgct tgtgtcataa cagctccatt
     9061 taggtctgaa caccacactc ctgaaataac aagagagaat ccagtaagag tacagattat
     9121 aatagtatct ataaaagtac ctgtcataga tataagacct tgttccgctg gccattcagt
     9181 tttagcagca gcagcagcta ttggagcact acctagacca gactcatttg aaaatacacc
     9241 acgagcgata ccatttctaa ttgccagtga catagttgct cctaaaaaac caccagtagc
     9301 agcagtaggt gtaaatgcac ttttaattat taacataaaa gtttcaggga ttttattcca
     9361 aaaagatatt aaaatgattc cacagattac aacatatata actgccataa aaggtactat
     9421 tttagtagct acattagata tacttttaag tcctccaaat acaaccaaag ctacaaatac
     9481 tgctaataca acccctgtaa ttctagggtc tatcccgaaa ctattattta ttgcatctgt
     9541 tattgaattt acttgagtaa aagtaccgat tcctaaaagt gcaactagta taccacttat
     9601 agcgaaaaat attgcaagag gtctccattt ttctcccata ccatttacta tgtaatacat
     9661 tggcccacca gacacttggc catttttatc tttagttctg tattttattg caagaacacc
     9721 ttctgaatac ttagtagcca ttccaaagaa tgctgctatc cacatccaaa aaagtgcacc
     9781 aggtccacca gctttgatag ctgtagcaac accaacaata ttaccagtac caactgtagc
     9841 agctagagca gtacataagg ctgcaaaact tgaaacatca ccactacctt tgttttcagc
     9901 agagaatatt aacttaagag ctcttggcag tttagttatt tgaacaactc caagcttaac
     9961 agtaagtagt ataccagtac caactaatag taccagtagg ggtggtcccc aaataaaagc
    10021 atcaactttg tttaaaatat caattagatt cataaaaaca ctccctcatt ttttttgctt
    10081 tacataatca atagaatttt taatgaagta attgacagga aataagtata atttattaat
    10141 aatttctagt gtaataaaaa aagctaaatc taaaaaagat tcagctctgg atgtacacta
    10201 attagcaacc aaaataaaag tattagtaaa tactgttttt ggtagataat ccctgtcctt
    10261 ttgcctgaga gattgagcat aaaagccttg ccccttcggc gcccataatg ggtctctcca
    10321 gagttccgta aattttacag tcctactttt aaaagtacct gagagtgtta ctccttcggt
    10381 gtgctatata gcaactcttc tataaaatct taaccggata ttgatttgtc atagcttatt
    10441 gtattaataa tattaatata agtttttatg ttttgtcaac taaaaaatta aaaaacaaag
    10501 aaaaaatata aaaatgtaag cctatttacg aattataaac ttagaaagat gagtaatata
    10561 cgtgaaaa
//
LOCUS       DACXMZ010000140        10477 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.141, whole genome shotgun sequence.
ACCESSION   DACXMZ010000140 DACXMZ010000000
VERSION     DACXMZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.141"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            174..977
                     /locus_tag="JD016_13280"
     CDS             174..977
                     /locus_tag="JD016_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAU5000603.1"
                     /translation="MMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVLK
                     LLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIVP
                     TSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLG
                     VIILGMCWSLWHLPLIFTLYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCAI
                     IHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(1076..2038)
                     /locus_tag="JD016_13285"
     CDS             complement(1076..2038)
                     /locus_tag="JD016_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAU5000604.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(2354..3304)
                     /gene="hpdA"
                     /locus_tag="JD016_13290"
     CDS             complement(2354..3304)
                     /gene="hpdA"
                     /locus_tag="JD016_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HAU5000605.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYGNGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWTQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(3297..3554)
                     /gene="hpdC"
                     /locus_tag="JD016_13295"
     CDS             complement(3297..3554)
                     /gene="hpdC"
                     /locus_tag="JD016_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HAU5000606.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(3558..6266)
                     /gene="hpdB"
                     /locus_tag="JD016_13300"
     CDS             complement(3558..6266)
                     /gene="hpdB"
                     /locus_tag="JD016_13300"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HAU5000607.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIKMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            complement(6563..7579)
                     /gene="tsaD"
                     /locus_tag="JD016_13305"
     CDS             complement(6563..7579)
                     /gene="tsaD"
                     /locus_tag="JD016_13305"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAU5000608.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVIQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDKLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(7585..8061)
                     /gene="rimI"
                     /locus_tag="JD016_13310"
     CDS             complement(7585..8061)
                     /gene="rimI"
                     /locus_tag="JD016_13310"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-protein-alanine N-acetyltransferase"
                     /protein_id="HAU5000609.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(8051..8767)
                     /gene="tsaB"
                     /locus_tag="JD016_13315"
     CDS             complement(8051..8767)
                     /gene="tsaB"
                     /locus_tag="JD016_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAU5000610.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYSRNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(8767..9219)
                     /gene="tsaE"
                     /locus_tag="JD016_13320"
     CDS             complement(8767..9219)
                     /gene="tsaE"
                     /locus_tag="JD016_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAU5000611.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(9358..10398)
                     /locus_tag="JD016_13325"
     CDS             complement(9358..10398)
                     /locus_tag="JD016_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000612.1"
                     /translation="MNLSKKIKKLFLDDKGYYFNSNWKDIHIGIYNTKLGDSLKYITN
                     EYIENLERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYICLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
BASE COUNT         3372 a         1911 c         1079 g         4115 t
ORIGIN      
        1 acttaaaatt aatatggata aatattctaa acccagtatt tattaaaaat gaaataaaaa
       61 gaaaaaaaat acaaattatt atctttttct ttataaactt tggtttaact tattcattag
      121 gtttactgtt atattataat aaatttattg atccagaaaa ctttgcatca ttcatgatga
      181 tattacctct atcttcagtt gctattgcaa agttttatac tgaaggatta aataatgata
      241 tgtatgaatt ttattcacta atcatattat tttttcttat ttattcatta ttaattgttc
      301 ttaagttatt aaatctaata aataataatg aatttataat tataaaccta atattaactt
      361 ttataagtag cttatcaatt ataatatatt catcaaataa aaaaaattta tatcctgtaa
      421 aaaatattaa actaggttct ttattaatta tttactttat gatttctgta attgttccaa
      481 catcatttat actaatacta caaggggctc aacctaatta tagtggtatc ataaaattta
      541 tatttactcc aataacatca ttaactacta tttatatttt ttttagtgaa gaatatgcct
      601 ggagaggttt tttacagaat attttttttg ataaatttgg taaaaagtta ggagttataa
      661 tattaggtat gtgttggagc ttgtggcact taccactaat atttacatta tatacacctg
      721 aagcgccaat attaggaata atattacgtt caatacatat agttggaatt tcaatatttt
      781 taggatatct ttatataaaa actaagaata tctggttttg tgctataatt catgttttaa
      841 ataatagtgc ttttcttatc acaaattcta aagaatcaac tatcacttac catgatatac
      901 taatagtatc tatggttgtt ttaatttttt atgttccatt tctatttaca aaagaatata
      961 aaaataactt ggagtaaata gaatatccaa gttattttta tatttttgca aatttccaac
     1021 aatcctacag aatactttaa gcttagaaga tattattaaa atttattata atcaactaat
     1081 tcttgatatt ggtcacattt actttatcac taacaaaatc atacttagaa tctctatagt
     1141 aataccatac aaatataaag ctaagtagat taacaaaagt agtcaaaatt ccaccttcaa
     1201 taccaaacat ccctccattt aatagattac catgagtgat ttgtgtagtt ataatccctg
     1261 gaacctgaag tccacttaca ggcataccat atataatccc ctgaaataca ttccatgtaa
     1321 aatgataacc tatacacatc catatattcg acgactttat aaacatatat gaaaaaagaa
     1381 tcccaataat tataatatta attacagaca aaaaagtaac atcaggattc catatatgaa
     1441 tcaacccaaa aataacagat ggaacaatca ttataaaata aatattttta catcttcgta
     1501 aaactgccat aaaaaaacca cgatttaaaa tttcttctgc aaaagcaact aaaataaaga
     1561 caagtatcca agaaactgtt aaaggtgaaa tttttggtac ccatgaagta acatgaccac
     1621 caccaattgt tattgttata ataaagatta ctgtacaatt aaatattcct aataacattc
     1681 ctacatatcc ttcttttttc ttaattctat aagaatgtaa ccccatttca ctaataggat
     1741 ttttcataat aaatctccat acaattatag gaataataat cataagtgaa tctgttagaa
     1801 tttgaaatac aatctgtaac ccaacatcat ttaaccaatt agcatattct gaaaaatagt
     1861 ttgtttttaa attaatgtca cctgtagcaa ccaggatttt tcttattatt tccacaagta
     1921 tcatgcttaa ataatactga agtatgtaaa aggtagccat aacagctata ataatccacc
     1981 ctgaacgtac ttttttgtct ttattcttaa taatcaagtt gctccatgat ttgttcatat
     2041 agatgtcttc ctcctaacta ttatttttag ttgtttctaa aatatattct attaaatttt
     2101 ttcatagtag tattttacca caaaacttct gcttcaatga agtaaacgta atatattctc
     2161 taactgtaat ttaaactata aaaaatatat aaagtatatt taaagcaata tttatggaca
     2221 taaagcaata taaacaatat ataaaacata taaaacttta cttaattatt tctacataaa
     2281 aaccacataa aaaaggatgt aactctagct tttagtgtaa catccttttt ttaattaaaa
     2341 cttgtattta attttagaaa gctgtctcat gaccaacata acaagctata ccattatcca
     2401 aaaatatatc ttgtaattct tcaagatgac cttcatcaac atcaccctta tcagaatact
     2461 catattcctt gcctaattga gtccattttg attctcctaa tctgtgaaat ggtaatagat
     2521 taatttctac caaattattt ttgtgcataa aagatataat atcacttata ttttcatcac
     2581 tatcattaaa cccagagata actggtactc taagtactaa tcttccattc caatcagaat
     2641 ttgcaaggtt tgatatattt tctaatatta aatcattata cacacctgtt tgttctttgt
     2701 gtttttctct atccatatgc tttatatcaa taaatgcaaa atcaatatcg ttaaatattt
     2761 tattaaaaac ttcatttgat acacatgcac tcgtttcaat ggcagtgtgt atatttacct
     2821 catgacactt taataatact tcatgtaaaa attcatgctg aagtagtggc tcacctccac
     2881 taaatgttac tcctccatta cttctccaat tgttagaatc acgtttaatt acctgcacaa
     2941 gttcatctac agtgtaaggt tttgcacata atttaaacgc attataataa catgagttga
     3001 cgcattcaaa actttcacag tccttacaga tgttccaatc tataactggc ttattatcaa
     3061 gattaaagct caatgcacca tttttacact taccatgaca tacagtacat ccatttccat
     3121 attgacaaga taattcacta aacatcatat gtggtcttac agtccaactt tctggatttg
     3181 cacaccattt acaactcagt ggacatccat ttaaaaatac agttgttcta caacctggac
     3241 catcatgaac tgaaaagctt tgtacatcaa aaatcatgcc ttctaattgc ttttgactac
     3301 tcattaaaca catgtccttc acaagtaatt gcattcaatg ttgaatatac ccagtcttct
     3361 ttctctaggc caacacattt acctatacct tcatcattag cttcaacaaa atttttacaa
     3421 tttttacatt ttggcattac ttttatctct ggacatgctg catcatctaa attaatcatt
     3481 tgttttgata atctacaaat tcctttggtt gcatctactg cacaaaaatt catacaatca
     3541 ctatgctttc tcatatttta caccccttca tactctgttc tagcaattac ttcatcttgt
     3601 attggtttac ctaattcaca ccaatattga gtaaacccag caacacgtac cattaattga
     3661 cgataattgt caggattttt ttgtgcatct tttaatgttt ctgaatctac aacattatat
     3721 tgaatatgga acccaccttt tcttaaataa cttcttgtta aatctaataa ttttttagtt
     3781 ccttgttgtc cttttatagt agttggatga agttttaaat tcatctgaga attttgaact
     3841 actgcttggt cccaaatagt tgcagattca aataatgcat atactccatt tttatctgtt
     3901 cctgcatatg cagagactga accatctgaa tatgttgttc cacttagtct accatctgga
     3961 gtagccaagg tagcagctcc ctgtggccca tgtgtagaaa cagatatttg gcaagggtac
     4021 atcttcttag catataaaga ttgtgcttct tcacatactt ttgataacca tacctcatag
     4081 tttttaagaa tattatctgc atacaaatca ttatttccat atttaggtgc atcaagacag
     4141 gccttataaa tagcatcata tttaccagtc ttatctactt ttacttggtc tgccatagaa
     4201 taatttccta cttctaatgc atctttaaat ccaaaattat ttaatatggc atccttcatt
     4261 tcctctattg taaatgcttt gtcatcatat acaagtttct taagagctgc aaatgaattg
     4321 accatagtaa cagtaccaca agtttctacg tttaatgttg cattataacg atatcccata
     4381 tttcctatat gttgaccttt gtctaaacag tctggcttta gtagtgaatt tattatagaa
     4441 gtatcaaatt ttctccatat atctaactca atgttatttg ctctttctaa aacatttatt
     4501 gcttgtttat aataatcttt aaatacttct atgacttctt cataagtatc taatggctta
     4561 ttatgaggct caaatacttg tatattcata cgatagtctt taccattcat aagaacaagt
     4621 tctaaaattt ttggattagc tataaagtga actccagagc cagcagattg acctgctcca
     4681 cctgcaatag agtatgtttt tccgtttaaa gttaactgtt tccaacaccc tggagatgtt
     4741 tccaaacatc cacctaatgc aaatgctctt gcttcttcaa cagtcatacc ttcatcagca
     4801 aattgtttca tcataaatgt tgttccattt gagttgttta cccatgctgg ataaccagaa
     4861 ccaagctttg tacattcagc agctttcatt aagaaatctt ctggaagctt ttcatcatat
     4921 agcatagtta aactaggttg aggagttcta catctcatac ttgcttctaa taataactct
     4981 tctagttcat tagctcctgt agaaccatct tcttttaatc cacctataga taaagtatta
     5041 aatgtattgc ctgatagtac tcctccattt acgcccgctg aagcaaaaca gtcaatacaa
     5101 gtgattttta ttctataaag ctctaataat tcgataacct cttcttttgt aattagtcct
     5161 ttttctatat cttgttcata ccaaggatat aaaatttgac caaaacgtcc tatagacata
     5221 ccagatatag catcctcatt tactgatgct atatgaattg tataagtcaa ctgaagagct
     5281 tccctaaatg tacgaggttg tttatgtgca atatgttcta atatctccac taaatctgag
     5341 tattcttttt tagcttctaa atctgtctct atactttcta aatattttgc atgttttgca
     5401 taatttaaaa tccaagtttg taatccttca ataacttgaa taacagcctc ataaaaatat
     5461 agacggtcca taccaataag cccatctcca gatgcatttc ctgcaactgc ctttttacat
     5521 tcttttgcca tttttataat tccatctaat ccataattta aaggataaaa ataatttata
     5581 acttctctac cttgaggaag tgtataccct gaatcaaaca tgcatataag tgtactcatt
     5641 aggttttctt ttaaatcata atctggcatc attttttcat agtgaaaacc taaatcttca
     5701 acagactttc caacccattc ctttgccatt tttacaagta caggtacttc ttcttttctc
     5761 attccgaatt taccagctat tgaaacgaca tttccaaaac tttcagtaac atttcctcca
     5821 ccagacccaa attttgtaag ttcatcagct gtgttactgg ctaatttctt tgcctcttct
     5881 ctcatttgtt ctccttgagc aacaaagaaa ctttcactta cccatggcat tggaaaagaa
     5941 cctctataat ggcgtgtctt ttgcattact attttctcac caggtattat atttggtgtc
     6001 atatgtgaaa aagcagcttt aagagcttta gctcttctaa ctactggtat atcaccatct
     6061 gattttctat attctctatt ataccaatat ggaaattcca tatcagcagt agataatgta
     6121 ttataatata catctaataa gtcttctgct cttttacttg cttttttttc attaaattca
     6181 cttaaatttt ctttattttg aaaatttgat ttaatatttt ttgcttccaa aatacttctt
     6241 attttgtctt ctttactttg actcatttct tcccctcctt aatctttcta taattagtat
     6301 aaaacttttt aagaatgaaa aaaaagggta tattacccac tttttaaaat aatatttcta
     6361 tagaaaaaat taaacttgaa gcaaatctaa gccaaagaca atctgtatat cctaaaattt
     6421 gttgcaattc attggaatat aaagatcata aatagtttta cttcaagttt taatatatac
     6481 tatatttatt tttattatat ttaatttcac ttctacttac ttttttgaaa caatactttt
     6541 atatttacaa attcatctaa ctctactgat ttatctttaa atttggaact gcatttaaac
     6601 tcatatcatg cgttttcccg tttataaaat tataatatcc tgcacaccct atcatagctg
     6661 cattatctgt gcataaaatt agaggaggat acttaacagt aataccattg tctttagcaa
     6721 gctctgttat tttagctcta agccccgaat tagaagcaac cccaccagaa agagttatga
     6781 tattgtatcc tttgtctttt actgctttta gtgctttagt agataaaacc tctactacag
     6841 cctcttggaa agatgctgct acatcttcaa ctactatttc ttcattcttc attctttttc
     6901 catttaaata atttaataca gaagatttta atccactaaa actaaaatca tagctatctt
     6961 cttctaaata ggctcttgga aattctatag catgtttatt tccattcttg gccagcttat
     7021 caataatagg acctcctgga tagcctaaat tcatagctct tgaaatctta tcaaatgctt
     7081 ctccagatgc atcatctctt gtttttccta aaatttcata cttgccataa tcttttactt
     7141 caaccaagtg agtatgacct ccagatacta ttaatgttat aaaaggaggt tttaaatcct
     7201 tatgctctat atagtttgca cttagatgcc cttctatatg atttactcct acaagtggaa
     7261 tattcaaagt atatgcaagt gctttagcat aagatagacc aaccaataaa gcacctacta
     7321 gcccaggtcc atatgtaact gcgatatggt ctatatcatt aaaacctata tttgctttgt
     7381 ctaaagcttc ttgaataact atatcaatat tttccacatg ttttcttgag gccacttctg
     7441 gaactacacc accaaatttt ttatgtgtct cgatttgagt agagattatg tttgaaagta
     7501 cttctctacc attttttaaa actgatgcag ctgtttcgtc acaactactc tctatagcta
     7561 atgttattat atcactcata aactctacac ctcttttaat tggttccaca ttataattgc
     7621 atcttcttta ttatcactgt agtattcttt tcttattcca cccattttaa atccatattt
     7681 tctatataaa ttttgtgcta ttttatttga tgctctaact tctaatgtca tagatgcaat
     7741 attattatct ttacataaat caactatgcc ttttatcaac ttgtccccta tttttttccc
     7801 tctatagtca ctatgaacag ccacattatt tatatgacct tcatcaagta taagccaaat
     7861 tcccacatat ccaacaattt ttccatttaa cttagctact atataccttg ctacttcatt
     7921 agacaactct tttctaaaag actcttttga ccaataatct tcaaaacaat ttttttctac
     7981 ttcaaaaact tcatctatat cttctgtagt catttcttct atttttatat catctactat
     8041 tagttttgta ttatcttcca ttatttagcc tcttcatttt ttcatcatat tgtacttcag
     8101 cttgagattt tctgatatac ataggattta tcgtataaca agtatgtaca tctatatttc
     8161 tactatattt atttaacgct aaagagcata agcttcctgc gttagataca ttattagctg
     8221 gtgaaggtat ttttatattt tttatattct ctaacttgtc tttatatttg tatacagctt
     8281 ctccaactat tataaattct tcattagttg atacaatttc atctactaaa gcatcaaact
     8341 ctacaacatc aacaccatct aattctatca tctctccatt ttcaaacttg tatttagctg
     8401 aataaacttg atttttctgc gcatctaata tacaacatat ctttctatca cagaaattta
     8461 tgttatttgc tagagattcc aaagaattta ctgctattat tggtatatta tttacatggg
     8521 ccatagcttt tactgtagcc atacctattc taagtcctgt aaacgaacct ggacctatac
     8581 atacagctaa taaatccata tcttttatac ttaaatcact catactcaac atattttcta
     8641 tcattggcat aagcttttgt gaatgagttt ttttgttgtt tactgtaaac tcacatatta
     8701 agttatcatc ttcaacaact gctacacttg ctgccataga agacgtatcc atacctagta
     8761 ttttcattat ttaatcaact cctcaactat ctctttataa aactctcctt ttggagttaa
     8821 tatcatttct cttccttcat ctttatatct caattctata ttcaaatatt cttttggaag
     8881 tatatcttct attagattag cccattctat tatacagatt ccgtttgaat ttacatattc
     8941 atcataacca atatcataca tttcgtcact actacctatt ctataaacat caaaatgata
     9001 caaaggtatt tttccttcat attcatttat tatagtaaaa gtaggacttg ttatataatc
     9061 ttctatccct aatgaatctg ccaagctttg tgtcattgta gttttaccag ctcctaaatc
     9121 tcctactaaa cagataacac tgccttcttt taaaagttta cctaatttat atcctatttc
     9181 tctagtttta ttttcatttt ctaaatatat ttttgccatc aattcatctc cattttgata
     9241 taatcgtcat gtgaatttat atcactgctt aattaattat agtataagcc tagattatta
     9301 taaagtactt gtctttatct ttttttatat ttcactattg atggctgtat ctacacctta
     9361 tatacaatca tcaatagcaa tttttacata ctcttttgct ttatggtttt gcttcaatgc
     9421 ttctttacat attctctcag attgctcttt tacatactta agagacttat agttctcttt
     9481 tactgcttca atacacattt tttctgtttg attttttata tacataagca tttttccatt
     9541 ttgtcttaca atctctatac atatatcttc tgtatactta tctatgtaac ttagtacatc
     9601 tttaatatta tattttttat ttaaatatct tatagcattt atacatattt cttctgtctt
     9661 attgttgaca aattgtagac ttcttccatc ttgtttgaca gcttctatac atattttatc
     9721 agtttgttta tttacatatt gtaatgccat accattttgt cttacggatt ctatacacat
     9781 tttttccgtt tgatttttta catattttaa tgctttataa ttttgcttaa tggcctctat
     9841 acataattct tcactctgtt ctctaacgta tgcaagacac ctgccatctt ttttaattgc
     9901 ttctaaacat atccttcttg tttgagtctc taaagccttt agtatactta caacatctat
     9961 attggaagaa tgacttattg cttttataat aagcccttct gttttatttc gtacatactt
    10021 aagtgcatta caatcctttt ttatagcctc aagacatata tattctgttt gctcttttac
    10081 atattcaagt gctttataat tatactttat tgcttctaaa cataattctt cggtttgatt
    10141 ttttacatac tctaatgcct tgtaattctg ccttattgct aaaatacaca taccatcagt
    10201 ttgattttct atatttttta attcacgata gtcatatttt accctttcca gattttcaat
    10261 atattcatta gtgatatact ttaaagaatc tcctaatttt gtattataaa taccaatatg
    10321 aatatctttc caattactat taaaataata ccctttatca tctaaaaata atttttttat
    10381 ctttttagat aagttcatga atatacactc ccatcattat taaattagtt attattaaaa
    10441 atttgattac taatctttat agttttttaa taataac
//
LOCUS       DACXMZ010000141        10414 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.142, whole genome shotgun sequence.
ACCESSION   DACXMZ010000141 DACXMZ010000000
VERSION     DACXMZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.142"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            141..1490
                     /locus_tag="JD016_13330"
     CDS             141..1490
                     /locus_tag="JD016_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAU5000613.1"
                     /translation="MSKYQKNVSMFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVV
                     GGAVGVSPTQITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVG
                     GNYGLASILGAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMA
                     GGVNSPTYASMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIIN
                     FDPIREATWFAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNIT
                     DKELSSGVIGSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSG
                     FVPKFGALMTTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMG
                     ITSVPAAQEAFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            1787..3148
                     /locus_tag="JD016_13335"
     CDS             1787..3148
                     /locus_tag="JD016_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine permease"
                     /protein_id="HAU5000614.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAADLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            3221..4615
                     /locus_tag="JD016_13340"
     CDS             3221..4615
                     /locus_tag="JD016_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAU5000615.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            4677..5147
                     /locus_tag="JD016_13345"
     CDS             4677..5147
                     /locus_tag="JD016_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HAU5000616.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            5150..7366
                     /locus_tag="JD016_13350"
     CDS             5150..7366
                     /locus_tag="JD016_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAU5000617.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            7449..8120
                     /locus_tag="JD016_13355"
     CDS             7449..8120
                     /locus_tag="JD016_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAU5000618.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            8483..9697
                     /gene="dpaL"
                     /locus_tag="JD016_13360"
     CDS             8483..9697
                     /gene="dpaL"
                     /locus_tag="JD016_13360"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HAU5000619.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            9824..>10414
                     /locus_tag="JD016_13365"
     CDS             9824..>10414
                     /locus_tag="JD016_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAU5000620.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         3890 a         1217 c         2146 g         3161 t
ORIGIN      
        1 attatatatg taaaacaatt atgttaaaaa attggcatta taattgcttc atattaagtt
       61 gagatgacaa aataaaaaaa gaaaggatgt tgtgtgagat aataataact gtcattaaaa
      121 aatagttggg agtgataacc ttgagtaaat atcaaaagaa tgtttctatg tttgatattg
      181 atggaaggcc accaatttta aagtgtttgc cactatcaat gcaacatata ctagcaatga
      241 tagttggaac tgttacagta cctatagtcg taggaggagc tgtgggtgtt agccctacac
      301 aaataacact tttagtgcaa tatgcattaa taatagctgg tatatcaaca cttatacaac
      361 tatatccaat aggaaatatt ggttcaagat tgcctataat ttttggagtt ggttttactt
      421 atgtaccaac acttgtagca gtcggaggaa attatggatt agcaagcatt ttgggtgcac
      481 aattaatagg gggagtggct acagtattaa tagggatatt cataaaaaag ataagaaaat
      541 tttttccgaa tatagttgca ggaacagtgg tttttacaat tggattatct ttgtatccta
      601 tagcaataaa ttatatggca ggtggtgtaa atagtcctac ttatgcctct atgcaaaact
      661 gggcagtagc ggctataaca ttagtggtag ttataggtct tagtcaattt gcaaaaggat
      721 atgcaaaatt ggcagcaata tttttgggaa ttatagttgg atattgtgta tctttagttc
      781 tgggtataat aaattttgac cctattagag aggcaacttg gtttgcgata cctaaaccgc
      841 ttgaatttgg gatggaattt gatatgcctg taataatctc agtagtaatt gtaagtataa
      901 tcaatgctgt tcaagcaatt ggagatttat cagctactac aatgggaggc atggatagga
      961 atatcacaga taaggaatta tcaagtggag taataggaag tggaatttgt actatgatag
     1021 gagctttgtt tggaggttta ccaccatcat catatagcca aaacgtgggg atggtagcta
     1081 tgaacaaagt aataagtaga tttgtgcttg gaatagctgg tatatttatg ttattgtcgg
     1141 ggtttgtgcc taaatttggt gcattaatga ctactatacc atattctgtt ttaggaggag
     1201 ctacaatatc tatatttagt atgataacaa tgacaggtat aaagttaatt attacagaag
     1261 agttgtcatc aagaaatatt accattgttg gtttggcaat tgccttaggc atgggaataa
     1321 catcagtacc agcagcccaa gaggcatttc cttcatttgt aaagttagta tttggagaat
     1381 ctccaatagt aattgcaaca ttggttgcat tcgttttaaa tctaataata ccaaataaaa
     1441 gtctagagga tgaggctaaa gaaagagctg ttatggaaga gactaattaa gatataataa
     1501 tttaaaagaa ttgtttaaat tatttagtat gagattgtta gattattttt gatagctatt
     1561 cattttaata aaaaatttaa caaataaata tataccaggc aattaatgtt catttaagtt
     1621 gaaaatcggg gagggaatat ttagaaattt gttaattttt tataaaaata gctcagttta
     1681 gctgtgttta tcggttgagc aataaattca tggtcataaa aatatttata gaaaataatt
     1741 ataaaaatat ttagtcaaga tatttatgta tgggaggatt tattttatga aaacacgtaa
     1801 tacttcaaaa tatgatgtcg atgggatacc accactaaag gaatcaatac ctttggcctt
     1861 acaacatctg ttagcaatga tagtaggaaa tatggtacct gcgatattaa tagcaaacgt
     1921 ggtaggactt aaccaggggc aagcaacaat gttgattcag ggttctatgt tagcagcaga
     1981 tttagcaacg tttttacagt tatatccaat acctttattt aaaggattta agttaggttc
     2041 aagattacct gtaatgatgg gaatgagtta tgtattttta ggagcttgtc tttcagtagc
     2101 agctaaagat ggtttagcag ctctatttgg agcacagatt gctgctgggg taatcgtatt
     2161 ctttgtagga tttggagtta agaaaattag acatatattc actccaatag tttctggaac
     2221 tataatagca tgtatgggac ttggactatt cgcaacagct ataaagaatt tagctggagg
     2281 agaaggcaca caaacatttg gaagcccaat aaacttcaca gtaggagtta tagttgcttt
     2341 tgtaataata atgataaata aatacggtaa aggtttagta aaaaattctt caatattaat
     2401 aggaatatta gtaggttatg ctatatcttt agttttagga ttggttgatt tctcagccgt
     2461 tcaaggagca gctatagtat ctttaccaac accagcagct tttggattag aatttagacc
     2521 agagttaata gtaatgttta ctattattta cataataggt atagctgata tgatgggtgc
     2581 ctgtacaata gctacagtag gagcaatgga tagagaggta actgacgaag aattagcatc
     2641 agttgtttta ggaaattcta taacttctat aatttcatca ctatttgcag ctcttccaac
     2701 tggagtattt agccaaaata cagtaattgt atctatgaac aaagttacaa gtagatttgt
     2761 aatagcacta ggggcacttg tattattact agcagggata tctccagcat tgggtgcaat
     2821 aatgacaact ataccatctt gtgttgttgg tggagcaact ttagtagttt tctcatcaat
     2881 agcaatgtct ggtttctcta ttatgagtat ggatggattc actgaagaaa acaatcttat
     2941 agcaggtgta tctatagcaa caagtatggg tcttacaact gctccacaag ttttagacca
     3001 attcccagaa acaattggaa ctgtccttgg agggtcaagt atagtttctg gagcaataat
     3061 agctatatta cttcaaacat tatttaaatt aaagtcacgt aaaagtgctg aaaacgttac
     3121 ttcaaatctt gaggaaaata ttggataaat gtattagata taatatgaga aaaccatatg
     3181 gtaaaatgtt gtaatgaaag tattcaaagg gggatttaag atgattgata tactaataaa
     3241 aaatgcaata attgttacag ttaataaaga cagagatgtt atatttgatg gagcaatagc
     3301 tattcaaggt gacagaatct tggacatagg aaatacagtt gatatcgagc ctaagtatct
     3361 tgatgctaaa aaagttatag atgctaatgg aaaagtaata ttccctggct ttataaatac
     3421 tcataatcac ctgttccaag tgttattaaa aggtttaggt gatgatatgg cacttcatga
     3481 gtggttaaat acaatgatgt ttccatcagc aaaattctta acagaacaag atacttatga
     3541 tgctgcaatg cttggatgta tggaaggttt aaaaagtggt ataactacaa tggttgatta
     3601 tatgcatact cacaatagac caggacttac agatggaata gtaaaggcat ataaagattt
     3661 aggcataaga ggggttgtag gtagaggttg tatagattta ggtattcaca aagagcttat
     3721 agaagatgtg gaaacggttg aaaaagattt gagaagggta ttcgaaaaat atcataattc
     3781 agaaaatgga agaataaaaa tctgtgtagc tccttcttca atgtgggcta tatctgaaga
     3841 aatgggtaag atgctttgga atatagttaa agaatatgat tcatatttta cagttcatat
     3901 atctgaaaca gaatttgcaa gaacagcaac aaaggatata catggagaaa tagatataaa
     3961 attacttgaa aaatggggta tagtaggtcc agaagttgta gcagtacatt gtgtatgtat
     4021 aactgatgaa gacatggaaa tgcttaaaaa gtatgacata aaagtttctc ataatgtagc
     4081 aagtaatatg tatcttgctt ctggtgttgc acctgtgcca gaaatgttaa aaaaaggtat
     4141 aaatgtgagt ttaggtcttg acggtgcagc aagtaataac gcacaagata tggtagagct
     4201 tatgaaatta acagctttac aacacaaagt aaataattgt gaccctctgg caatttctgc
     4261 agagaaagtt cttgaaatgg ctacaattga gggagcaaga actcttagaa tggaagatga
     4321 aataggttct ttggagattg ggaaaaaagc tgacctagta atatttaact caatgctttc
     4381 tccaaaatca atacctcttc acaatcctgt atcaactttg gtatactcag ctagtatgca
     4441 caatgtagag agtgttttag ttgatggaaa tgtaatactt gaaaatggaa aagtaactac
     4501 aattgaaagt gaagaacaag tgttgcttaa tgcacaacat gcagcagaag aattatgtaa
     4561 gagagctaat gtaactaata gaatggaagg acataagtgg aatagcttat actaatatta
     4621 aaataagatt gtaataatat tctattacaa cattagttta tctgtaggag gagaaaatgg
     4681 aaaaaataaa attaaatatt aatgattaca tgtatgaaat agaagttgaa aagaatgaaa
     4741 ctcttttgca tgtacttcgt gaaaaattaa atctaactgg agcgaaacaa ggttgtaata
     4801 gtggcagttg tggagcatgt aaagttttga tagatggagt tgacatgaaa tcttgtaaac
     4861 ttcttgcaaa aaactgcgaa ggtaaagtta tatacactat agaaagtttt gctaatggag
     4921 aaaatcttca tccaatacaa gaagctttta tagaagttgg agctgtacaa tgtggatatt
     4981 gtactcctgg tatgattata acaacacaag ctcttttaaa tagaaatgca tctcctaatg
     5041 aagatgaaat tagagaagct tttaaagaaa atctctgtag atgcacagga tatgtaaaga
     5101 ttatagaagc tgtaaaatta tctgcagcta gattaggggg tagataatta tggaaaaggt
     5161 aataggaaaa tcataccctc taaaagatgc agctttaaaa gttactggtc aaatgaaata
     5221 cgtggctgat atgaaaccac aaaatgtact ttacgctaag atgcttttaa gtcctgtagc
     5281 acatgcaaaa ataaaaagta tagatacaag tgaagctgag aaagtagtag gtgttagagc
     5341 tatagctact tatctcaata catcacaaag aaaatacaat ggttcattaa gattttatga
     5401 gcacaaaata cctgaaaatg aagttatatt ttcagataca gttagatttg ttggtgaccg
     5461 tgttgcagca gtagccgcag agactccaga aatagcagag aaagctgtaa aacttataaa
     5521 agttgaatat gaagaattgc cagctgtatt tactatacaa gatgcaataa aagaagatgc
     5581 tatctgcata catggagatt caaatatagt ttctcaaaac aacataaatg gaggagatgt
     5641 agaaaaaggc ttcaaggaat gtgactatat atttgaagat gaatactcta ctccagcaat
     5701 acatcatagt gcaatagaga ctcatagtgt aattgcacac tatgattata atgataaatt
     5761 aactgtacat acaccttgcc aaaatacatt tggatttaga ataatactat ctcaaatttt
     5821 tgggatgtca ttaaataaag taagagttat aagacctgct attggaggct catttggtgg
     5881 aaaattagaa cttacaattg aaccagtagc agccgcatta tcaaaaatga caagaagacc
     5941 agttaaaatg gttcttacaa gaaaagaatc tatgatatcg acaagaactc gtcatgggtc
     6001 ttttgtaaaa ttaaaaactg gtgtaaataa agatggtgaa gtaatagcac aagacataaa
     6061 aatatataca aatacaggag catatgcatc aagtgcttta aatgttatag gagcacttag
     6121 tcataaagta tttaaagttt ataaaatacc aaatataaag tttactggga tgcctgtata
     6181 tacaaataca ccaatagcag gagctatgag aggctatggg tcacctcaaa tatttatggc
     6241 tcaacaagct caatttgcga aaatagcaaa ggaaataggt atggatttag tcgattttca
     6301 aaataaaaat gcagtagagc cagatgatgt cgatataatc ttccacggtt ctcttggaaa
     6361 tcctagagta ttggattgta tagagcaagg taagaagatg tttaagtggg atgaaaagaa
     6421 aaaacttcca aaagaagaag gaaaatattt aagaggtata ggtatggcaa taggtgcaca
     6481 tggtaatgga gtttttggtg cacatagaga tgttgttaca ttgactctta aattaaatga
     6541 agatggtacg ttgacactac taacaggagc tcatgatatg ggtaatggtg ttgtaactat
     6601 gcaaacaatg atggtagctg aagttttagg aataacacct gataaagttg atacatttga
     6661 aactgatacg gatgcatgta cattcaacct tggtgactat gcaagtagag gggtatttgt
     6721 agaaggtgga ggagctaaaa agacagcaga gaaattgaaa aatcttatat tagaagaagc
     6781 agaaaaatta cttgaaacat caaaagaaga tttatatcta gacaatggat gtgtaattag
     6841 taaacttgat gaaaatgtaa aagcatcact ttctgatgtc gcagtatata gtcaaagtaa
     6901 atcattaaga gagttaactg ttgttgaaga ttattcttca ccagctggac gtacttctta
     6961 tggtgtacat tttgctgaag ttttagtaga taaagaaact aaggatataa aagtagttga
     7021 ttttgtagct gtacatgatg ttggtagagt tataaatcct ctaaatttag aaggacaatt
     7081 agaaggtggg attcatatgg gtctaggata cgcattgagt gaagaattgg catttgatga
     7141 acaaggtaga cctaaagcta ctgattttaa aacatataaa ttatttagaa ctgtagatat
     7201 gccaaaatgt caagttgcat ttgtagatgg ttatgaaaaa gctgggcctt ttggtggtaa
     7261 gagtattgga gaatgtgccg ttgtaccagt tgcgccagct gttgcaaatg cagtatataa
     7321 tgcaactgat gtagagatac attcattacc aataaaatta aaatagtaga aattaaagca
     7381 gaaataacaa tttattgtat ttgtaaatag ataataaaaa tagatttatt tggtgaggag
     7441 atattattat ggcaaagata ttaggtattt gtggaagtcc acgtaaagga gcaacagaat
     7501 atgcagtgtt agaagctttg aaagaagcag aaactatacc tggtatagaa actgagtatt
     7561 ggtcagttag agggaaaaaa ataagtcctt gtgtacattg tgatgcatgt ataagaaaag
     7621 aaactatgtg tattataaaa gatgatatcc aagaattaga gcaaaaaata ttggaggcag
     7681 atggaattat agtaggttct ccagtttatg atatgaatat aactgctcaa cttacagcag
     7741 tatttaatag acttcgtcca atgtatttag ttcatccagg aaaacttcaa aataaggttg
     7801 gtgcagctat aactactggt ggaactagat atggtggcca agaattgaca aaacttccta
     7861 taataaattt cttcctaatg catgaaatgt tagtgtctgg aggtttaggt ggatgctata
     7921 taggtggtac aatatggagt aaagatggaa aagcaaaagg tgcccaagag gatgagacag
     7981 gtatggacac agtaaaaaga cttggaaaag gtgcagcaga ggcagttatg gtttctaagt
     8041 ttggtcttga aaaatggaat gtcgtaaaag aagaattaga tgttaaaaaa gatgaaaaat
     8101 cacctcttag agaccactaa agatttatta tattattgaa tatatacaaa tagtgatgta
     8161 atagattatt gcatcactat tttatgtttt aatttagatt ttataaattt tatattaaaa
     8221 aatatagaaa caaagtgttt tgagatttat ttttaaattt taaaaatgag aattaaaaag
     8281 tgagaagata gaaaaggttt tttagttatt aaattttaca tacattgtat gaactaaaga
     8341 tagaaaagaa aaaataataa aaacattgca atttctatac taaagagtca tggaaaaata
     8401 tctagtatat atttagtatt ggcatgttaa ttgcttatat attaaagtat aaaaatttaa
     8461 caataatttg gaggtatttt agatgttaaa agaaattaaa tggaaagtaa ataatttgcc
     8521 aaagggcgat aaggaaaatt gtatcaaatt tttaaatgaa gaagaaataa ctaaggttag
     8581 aaattttcac aaaagctttc ctcaatataa agaaactcca ttagcaaacc tagaaggttt
     8641 agcaaaaaaa ttaggtgttg caggagtata tgtaaaagat gaatcttata gatttggatt
     8701 aaatgcattt aaagtattag gtggttctta ttctatgggt agatacttag ctcaaagatt
     8761 agatacagat atatctgagt tagggtatga caaattgact tcgaaagaaa taaaagaaaa
     8821 attaggagaa ataactttct ttacagctac agatggaaat cacggtagag gagttgcttg
     8881 gacagctaat aaattaggtc aaaaatctgt tgtattaatg ccaaaaggtt cttctgaatt
     8941 tagattaaat aaaattaaag gagaaggtgc agatgctagt ataacagact taaattatga
     9001 tgatgcagta agattagcta atgattatgc agaagctgat gaccatggag taatggttca
     9061 agatacagca tgggacggat atgaagaaat accagcatgg ataatgcaag gatatggaac
     9121 tatggctcaa gaagctatag agcaattaaa agaatatgga gtagatagac caacacacgt
     9181 atttgttcaa gcaggagttg gttcacttgc tggtgcagtt caagggtatg ttgcttcaat
     9241 atatgatgaa tgcccaataa cagtagtagt agaggctgat gaagctgatt gttactataa
     9301 atcagcagaa gctggagatg gaaaaccaag atttgttgga ggagatatgc caactataat
     9361 ggcagggctt gcttgtggag agccaaatac tataggattt gaagtattaa aaaatcatgc
     9421 agcagcattt gtttcagcac cagactgggt ttctgctaaa ggtatgagaa cattaggtaa
     9481 tcctttaaat ggtgatgaaa aggtaatttc tggagaatct ggagcagtta caactggtct
     9541 tttagtagca gctatggaaa gagaagattt agcagattta agaaaagatt taaaattaga
     9601 tgaaaattca agaatacttt taataagcac agaaggagat actgaccctg acaaatatag
     9661 aagtatagtt tgggatggag aatatccaag tatataagga attactaaaa tgcaattatt
     9721 agatattggt ttacatattt agaagatata taagttctaa aatttagaaa atatataagt
     9781 atataaaata catacattat aaaaaatcta gggggataat gaaatgttaa acgaacaaag
     9841 aaaacaagaa gtaactgaaa tatgccaaaa attaattcaa aatcctagtt cttctggaaa
     9901 tgaagaaggt gttgtaaaag caatagaaga gtcttttgaa aaattaggtt ttgatagttg
     9961 gtcaagagat agatatggaa atatagtagg atgtataaaa ggtaacaagc caggtaagaa
    10021 aatattattt gatggacata tagatacagt tccagttcca gatgcaagta aatggagtgt
    10081 tcctcctttt gaaggaaaaa tagttgatgg aaagatatat ggtagaggaa cttctgacat
    10141 gaaaggtcaa tatactgcaa tgatgtctgc tgtagcgtat tttgctaaag acactaatag
    10201 agattttgct ggagaactat atgtagcagg tgttgttcat gaagaaatat ttgaaggtgt
    10261 ttcggctaga gagataagta aggctgttca acctgactat gttgtaatag gtgagtcttc
    10321 agagttaaac ttaaagattg gtcagagagg tagaggagaa atagtagtag aaacttttgg
    10381 taaaccagct cactctgcta atccagaaaa aggt
//
LOCUS       DACXMZ010000142        10282 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.143, whole genome shotgun sequence.
ACCESSION   DACXMZ010000142 DACXMZ010000000
VERSION     DACXMZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.143"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            26..1819
                     /locus_tag="JD016_13370"
     CDS             26..1819
                     /locus_tag="JD016_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAU5000621.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(1900..3282)
                     /locus_tag="JD016_13375"
     CDS             complement(1900..3282)
                     /locus_tag="JD016_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAU5000622.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISLLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            3540..3887
                     /locus_tag="JD016_13380"
     CDS             3540..3887
                     /locus_tag="JD016_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAU5000623.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            3905..4939
                     /locus_tag="JD016_13385"
     CDS             3905..4939
                     /locus_tag="JD016_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000624.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(4988..6037)
                     /locus_tag="JD016_13390"
     CDS             complement(4988..6037)
                     /locus_tag="JD016_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAU5000625.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            6312..7847
                     /locus_tag="JD016_13395"
     CDS             6312..7847
                     /locus_tag="JD016_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000626.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(8032..8718)
                     /locus_tag="JD016_13400"
     CDS             complement(8032..8718)
                     /locus_tag="JD016_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HAU5000627.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            8843..10225
                     /locus_tag="JD016_13405"
     CDS             8843..10225
                     /locus_tag="JD016_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAU5000628.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKEIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIQEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
BASE COUNT         3972 a         1283 c         1693 g         3334 t
ORIGIN      
        1 actattattt cgaggaggtc ttgttgtgaa tataaaagat agattaagtg gattaagaaa
       61 atttatggaa gaaaaaaata tagatgccta tatgattcca tcttctgata atcaccaaag
      121 tgaatatgta ggagattact tcaaatcaag agaatttata tcagggttta atggttcagc
      181 agggacggtt attgtgacta aagacgaagc tgggttatgg acagatggaa gatattttat
      241 acaggcagaa tctcaattag aaggaagtac aataaaatta ttcaaaatgg gacaagaagg
      301 ttgcccaact acagatgaat atttatataa aaatattcct gaaggaggaa ctttaggatt
      361 tgatggtaga gttatatctg ctagagaagg agctacatta gctgaaaaat tatctaaaaa
      421 aggtataaag atagaatatc agtatgattt aatagatagt atatggccag atagacctgc
      481 tttatcagat agtaaagcat ttttattaga tgttaaatat tgtggagaaa gttttagctc
      541 aaaattagct agattaagag aaaaaatgag tgaaaaaggt acttctactc atgtaataac
      601 tactcttgat gatatagcat ggttattcaa tataagaggt ggagatgtta aatataatcc
      661 agttgtactt tcttatgcag taataacttt aaaagaagtt tatctatttg ttgatgaaag
      721 taagttaaat gaagaaatac taaatgaact agctaaggaa aatgttcaaa taaagccata
      781 taatgatgtg tatgaatttg taaaaaatat agacaaaact gaaaaagttt tattagatgg
      841 aactaagctt agttatacaa tttacaataa tataccttgt gaagttgaga aagttgatga
      901 gttcaatcca gtaatgtttt ttaaagctca aaaaaatgag gtagaattag aaaatataag
      961 aaattcacat gtaaaagatg gtgtagcttt cactaaattt atgtactggt taaagaaaaa
     1021 tgtaggtaag atggaaatca ctgaaataag tgctactcaa aaattagagg acttaagaag
     1081 agagcaagaa ggtttctttg agccaagttt caatacaatt gctgcatata aagagcatgc
     1141 tgcaatgatg cactatagtg caactccaga gtcaaattat aaattagagg cagaaggatt
     1201 atttttagta gattcaggtg gacaatatta tgatggaact actgatataa ctaggactac
     1261 agttttagga cctataagtg atgaattaaa actacacttt acttctgtag caagaggaat
     1321 gataaatctt tctaaggcta agttcttgca tggttgtaga ggatataact tagacatact
     1381 atctagaagt tgcatgtgga atatgggaat tgattatcaa tgtggaactg gacatggtat
     1441 aggatttgtg ctaaatgttc acgaagctcc aaatggattt agatggagag tagtacctga
     1501 aagatttgat agtgcagttc ttgaagaagg aatggtaact actaatgaac caggtatata
     1561 tatagaaggt tctcatggta taagaacaga gaatgaaatt gtagttagaa aagctgaaaa
     1621 gaatttctat ggtcaattta tggaatttga agttgttacg ttagctccta ttgacttaga
     1681 tggtatagta ccagaattaa tgaataagga tgaaaaagat tatttaaact ggtatcataa
     1741 gttagtttat gataaaatat cacctttctt aactgatgaa gaaagagaat ggttaaaagt
     1801 ttacactaga gctatatagt ttgtactaga tacaataaaa atcgaggtat tataatgcct
     1861 cgatttttta ttgtatattt tattaacttt aaaaaatttt taatttgata attctactaa
     1921 ttttttattc attataagta gtataattcc aaatatcaat aaaaatgatg gtatcactat
     1981 gaagacttgt aacataccaa gttggtctat aatagcttgc gtatagccag ataatttatt
     2041 tgctgcaaat ttgcctaaaa accaaacacc cattagtaat gaaatatact tttcaggcga
     2101 aagtttattt accataccat acccgactgg agaaaagcaa atttctccta tactttggaa
     2161 tgttaaaaat ccaactaccc aaaatacact tgctttgtga attgaattag ggtctgttcc
     2221 tgtttgtata actgctacta tcataaacaa aaatgctatg gctaatagta taaaacctaa
     2281 gctcattttt tttggcacac ttaaatcgcc ttttttagtt tgagataatt ttacccatac
     2341 taaagcagag ataggtccaa gaataacaca aaggagacca ttgtatgaat ctatccatga
     2401 agtaggaatt tgaaaattgc caacagtaag atttatgtgt tcctttgtat agagtgcaat
     2461 agacatagag gcttggttat atgctatcca aaaaaatact gtgaaaaaag ctaacaccaa
     2521 tattactatt attctttttt tctcaatcaa agttaaaggt cttgaagcga gcattttctt
     2581 atattctagt gattttctta tagactctct cccgttaata gggtcattat tcaaaggttt
     2641 ataaaacttt ctgtttccaa atataaagaa gaataaacct attagaaccg taattgatga
     2701 aaataaaaat acatatctat atccatacat acttatagat aatccgcttt tcatagcaaa
     2761 ccattcatta gcaactaaac cgcatattaa agggccaaat aaagtaccta gattagtagc
     2821 catatacgta atagagaaag ctccatcttt tcttgggtca tcattggtat ataagtttcc
     2881 aaccatagta tgtaagtttc ctttataaaa acctattcct aatccagata aaaatatcat
     2941 gatgtttata tccatcttac tatttgctat gtatcctaga aaataaccaa ttgaaaccat
     3001 tagcattcca agtataatac agtttttggg acctaaaaat ctatcactga cccatccacc
     3061 aataactgga gagaaataag tccatgctaa aaaagatgca agcattgatg cggattctgc
     3121 tggtgttaac cctaaaccac ctttggagac ttgttcagct aaaaacaata ataataccaa
     3181 tttcatacca tagtatgcaa gtcctacaaa ggtaaatatt gaacagcaga aatataaatc
     3241 tctaggttgt ttaagttttt cttgcgtttt taaaactgac atatgtcgag aacctccaga
     3301 taaatagttt aacaaaattt taccatacta tatgagtata tgtttttttc atatagacgt
     3361 caacttgtaa gataatttaa ttaagtcgaa atatatgtaa aaatagtatc atttttgtgt
     3421 attcagtaat atttttttat gataatatat gttattatta tattaaaata caaacatatt
     3481 tatggaaagg atatgccaat ttagtagtta gattgattgt ttggcaggtg ggatgagcta
     3541 tgataagaaa gaaatctgaa ttacaagtag aagtaaaaga aaatttaaat ggtggtatag
     3601 gaaaggttaa gcttgaaaac ataatacaaa atgaagagct taaaggaaaa ggaagactat
     3661 ttaaaagagt agctttacca gtaggaagtt caattggtgt gcatgaccat acaacagatt
     3721 ttgaagtata ttatatacta aagggaaaag gtaaagtatt tgataatggg gaatttgtag
     3781 aagtaaatga gggtgatgta gtgtatacag ctgatggcga aaaacattct atagaaaata
     3841 taggagaaga agatcttgag tttgtagcac tagttttata tatgtaaaat atggaggagg
     3901 agtcatgaca ggcaagatag aagaaattaa ggacaagata aatagatttt gcaactccaa
     3961 tttaaatcaa gaatacaaag atataagctt taaaattttg caaaaccttt tagataataa
     4021 taaagagata attcattctt ctagagctga tatatggtct tcagctatac taaatatagt
     4081 tttagaacaa aatcttttat acaataagaa gcatccacta catataacaa aaaaagagtt
     4141 ttcaaaacaa ataggtgttt ctttaaatac tataaataat aaatcgtctt taataaaaga
     4201 tgtttgtagt attgactttt taaatcaaga tactatagct atttctaatg tagaattttg
     4261 ggtaaaagag actaattcca aatcaaagtc tgtagattta gatttagaaa agaaaaaaat
     4321 actatataaa agatatatta agctatcaca agatgcgaat agtgatattg aatcaattag
     4381 atatatggag gaagctgtaa atataggtaa aagtatgatt actaaagaag ataaagaact
     4441 tggattttgg aaaggtttat ctaccagagc ctatatgata gcacttgaat cccttgctag
     4501 aaagttagaa agtattaata atctaaagga gtccaaaaaa gtattagaat atttaataga
     4561 aattaatcct agtgatgagc agggaataag atacaaatta ttaaatgtat taattagatt
     4621 aaatgataga acttcaataa ataaattatt tgaactttat aaagaagaaa aatctgctac
     4681 atggatgtac agtaaagctc tttattactt taaaaataaa agtatgtttt tggcaaatga
     4741 ttctattaag gttgctagaa gtaaaaataa gtacgttgga ttatatttgt tagattggcg
     4801 aaatgctttt ggtagagaat ttgttacaga ggaagaaaag gcagaagctg tctattacta
     4861 tgatgaaaac atagtgattt ggaatgaagt taaaggttct atggattggc tattaaaaaa
     4921 aatgttggaa ttttcataaa aaaaactact ccttactttt atataagtaa aaggagtagt
     4981 ttttaattta tacatctagt tttaaatctt caggttttat tagacctttt tttctcataa
     5041 attctgaaat caacaaagtt acaatagcag gaagtataaa attaagtaaa agtataccaa
     5101 cccacataga tatacctcct tttccagcac tttgcatagc agttactgtt ccaaattgtc
     5161 caaccaatcc acaagtgccc ataccagaac caataggaat attctccatt ttgaatattg
     5221 ttgtagataa tggtccaagt aatattgaag ctacagtagg tggtatccaa attttccaat
     5281 tttttattat attaggtact tgcaacatag atgtcccaag accttgtgcc acaaggccac
     5341 caactccatt ttccctaaag ctcataacag caaagcctac catttggcaa caacaaccta
     5401 cagttgctgc cccaccagca agaccaccta aacttaacat catacaaata gctgcactac
     5461 ttataggtaa tgtaagagct attccaacaa gtgcagagac taaagctccc attattatag
     5521 gttgcatagt tgtagcttgc attattatat ttccaaatga agtcataaaa gcagaaacag
     5581 ctggaccaat aagttcagct acaagtactc caattagtat tgttacagca ggtgtaacca
     5641 atatatctat ttttgtttct tttgaaacta attttccaac ctctacagca aaaagtgtag
     5701 cgacaaatgc tccaacaggt cctcctaatt cattaccaca agctccaaca agtgcacaag
     5761 aaaatagtac aagtggaggt gcttgaagtc catacgcaat agccactgct atagctggac
     5821 cagtaacctg tttagcaaga gggcttataa cttcagtaaa aagagaaata ttgaaattat
     5881 ctcctaatgt attgaaaatt gttccaatta aaagggacga aaaaagaccg agtgccatat
     5941 aacttagtgc gtcaataaga tacctttgaa cagaaatttc aatatttttt cttttagcaa
     6001 atgatttgaa atttcttttt ttataattga tttccaattt aaaatcctcc tcatataata
     6061 taagtataat ttctttttaa ttaattatat aacttttcag ctgacaagac aaatgttatt
     6121 tcactaaaat tagactattg taatagtaag aaattaacat taattttagt tttaaatttt
     6181 aaatttatgg tttataatta gtatagggat attctgaaat ataataattt tccagtttat
     6241 cccaatattt aacttaggta gattgcttac aaaacaaatg attatatatt aggctaaaag
     6301 gaagtggata gatgatattt gtaaaaaata agaaatacag aaaaattgcc ataattatat
     6361 ctattcctat attgcttgtt gcgcagtact acctttttaa atttggaata cttagaccaa
     6421 tggtaaaggg agtagaaatt gaaattgtag atggggaata tgttaaagat atagataaat
     6481 atgtagtaaa attaaatgaa acagttactt tgtctacagg tgaatatatt aaggttcctt
     6541 cgtatgctaa aaatccaaat atttggttta atgtattgga taactcaggg actttaaaaa
     6601 tagatggaaa taaaatgaca gcattaaagg ttgggtattc ttctgtgggt atcatgaaaa
     6661 attcaagagt attaaaaaag gctactataa aagtagtaga cccagaggta gaatcactag
     6721 atatggagat taatggggat ttaaaacatg ttggtgatag tgctactata gaaagtacaa
     6781 tagaagttga ttatgataag tttaaaaaat catataagcc agtttataag agtagcaatg
     6841 aaaatatttt aaaaatcaaa ggaaataaag taagtgcagt tggagttggt aaagctacaa
     6901 tatatgctaa gtgtggaaat aaagaaattg agagaacatt taggattcaa gctagagttg
     6961 ctgaaataaa tatggatgat ataaaaatag aagttgacca gaatgaaaaa ttgaaaccag
     7021 atataataac ttctccacct ggtttagaac caccaaaaat atattatgag ttagtagatt
     7081 caaaattacc tgtagaaaga gcaataagtt taagtagtaa tggaaatgtt gtagggctta
     7141 gagaaggtaa agatagggtg agaataactt gtggagataa atctaaagta ataacagtaa
     7201 cagtagtcaa agaaactata acgaatgact atatacaaaa cttggttggt tcacataaaa
     7261 tagtaaacaa taaagttatt attaaacttg aatgggatta tatgaaagat gtctttgatt
     7321 atgatatata tcttaaagat aagttgtcag gagaatcatt taagaaaatt aaatctataa
     7381 ctataaatga aagtgattta ggtaagtcta acaaggtata tgcgactata gaagtagata
     7441 tgaagggtaa tcaaaatata gattttgata tttatgtcat agggaaaaag gattcagata
     7501 caactaagcc aagtaatgta ataaatatta ccaatggaaa tggaaattca aataatgata
     7561 ataatattaa agatatgagg gtagaaaata tatcagctaa tgttgataga gataataatg
     7621 tcattagagt taactggagt agtatatctc ataaggactg tacttacagt atatatgtaa
     7681 aaaataatac taatggagat ggagaattta ctctatatca aagtgatata cagggaaatg
     7741 agcatactat aagtattcct gaaggagatt taaatctaga tatttatgtt gttgcaaatt
     7801 ataatggaaa tagctctcaa aatagtgatg ttataaatgt aagatagatt tgtatacaaa
     7861 ttgaaagtgt tgagtttatt taaatattaa attaagaaaa aggatagtat agggtaatta
     7921 tactatcctt ttttgttaca atatataggt ttaaaatctc tagtaatata aattataaaa
     7981 agtcttattt acacaaatct atagatattt tttttattaa tcaatattgt cttaaattga
     8041 acttagtgca gcttctattg catgtattac tgtcgtatca aatagtggaa tagaagtgtc
     8101 attttgttta attaaaagac ctatttctgt acatcctaat ataacagcct ctgcaccttg
     8161 tttgccaagc ttatctataa tagataagaa agcttttttt gaagattcag atataattcc
     8221 taagcaaagt tcattaaata ttgtgtcatt tactattata cggtcttctt catttggaat
     8281 taagacttca attccattat taataattac atttttataa aaatcttgtt ccattgtata
     8341 tttagtacct aataaggcca ctttttttat ttctttttcc cttaataccg tagcagttac
     8401 atctgctatg tgaagtaatg gaatatgtat actttcttgt attttatctg ataccttatg
     8461 catagtatta gtacagataa caataaaatc tgcccctgcc tcttgtaatt taattgcagc
     8521 atcagctaaa attttggcgc ttttttccca atttccacta gattggcatt cttcaatttc
     8581 ttgaaaatct acactgtaaa gtatacattt agaagagtgt agtccaccta atctttcttt
     8641 tattactgta ttaataactt gataatatgt aatggtactt tcccaactca taccaccaat
     8701 taagcctata gtcttcatat tatacctctc ttatttattt attttgaaat agctatttac
     8761 aaatacaagt atagcatcta aaatagaagc atacaataat aaaattgtat ggagtacaat
     8821 tataattaag aggagtaatt atatgccaat aaattctttt gaaaactatc ctatgacatg
     8881 gaaaccaaag ttagataacc gaaaaccacc aatatataaa actttagcaa tgttattaga
     8941 agaagatatt aaaagaggaa atttaaatcc tggagataaa ctgcctccac aacgggaatt
     9001 agcagatttt ctagatttaa acttaagtac aataacacga gcatttaaac tttgcgaaga
     9061 aaagggacta atatgtgcaa aagtgggaaa gggaacattc atttcttcgg atgtaaatgt
     9121 aagtaataca ttattatatc aaacggaatc aaaagaaata attgagttag ggacagttca
     9181 tcctccatat gagcaaaata catatattat tgactttatt aaaaatgtac taaaacaacc
     9241 agaaatggat aggtttttac aatacatgtc tccatctggc acatatatgc aaaaaaagtc
     9301 aattgcaaaa tggatagaaa gaaataatgt atacaccagt gaagaaaata tattattatc
     9361 tacaggaggg caaaatgcta tatgtgcaac tttactagga ttatttaaag ctggtgatag
     9421 aattgctaca gattctttaa gtttttctgg aattaagtca atagctaaaa tgataggtat
     9481 tcaattagtc ccaattcctc aagaaaataa tgaaatatcg attgaatatc ttgaaagtta
     9541 ttgtaaaaat gaaaatataa aaggcatata tcttatacca gattatcata atccaacgac
     9601 acatactatg tctgatttat ctcgtaaaaa gatagccaag atagcaaagc aatataatat
     9661 aataattatt gaagatgcta taaatagcat ttttagagac ggtatacaaa ctcccatatt
     9721 ttctttagct agtgataata ctatatatat ttttagtaca tctaaatttt tatgtgctgg
     9781 tcttagagta gcatttgtag ttgctccaaa acgatatata gaaaatttag aaaatgcact
     9841 atataatatg aatctaatgg tttctccatt tacagcagaa atagtacacc gtttattata
     9901 ttctcctatt atagataaaa ttatacaaga gaaaagagat gcaattatag aaagaaatga
     9961 agttgctgat aaagttttgt cagattataa tttgattggt gataagaatt gcagttttcg
    10021 ttggttactt cttcctgatg aattggatgg aaaatcattt gaaatttctg caaaaaatct
    10081 aggagttcaa gtatattgtg ctgaacgatt ttcagtagga aactctacag ttccaaaagc
    10141 agttcgtatt tgtgtaacag ctccaaaaga tgtagaagaa ttagaaaaag ggcttaatat
    10201 aataaagtca cttcttaaga gttaaattta ttttactatt atttaattaa ataaataaaa
    10261 aggcggagaa atttattata at
//
LOCUS       DACXMZ010000143        10279 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.144, whole genome shotgun sequence.
ACCESSION   DACXMZ010000143 DACXMZ010000000
VERSION     DACXMZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.144"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            186..1610
                     /locus_tag="JD016_13410"
     CDS             186..1610
                     /locus_tag="JD016_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HAU5000629.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1645..3774
                     /locus_tag="JD016_13415"
     CDS             1645..3774
                     /locus_tag="JD016_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAU5000630.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYNKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNIVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDK"
     gene            3804..4226
                     /locus_tag="JD016_13420"
     CDS             3804..4226
                     /locus_tag="JD016_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAU5000631.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4245..5396
                     /locus_tag="JD016_13425"
     CDS             4245..5396
                     /locus_tag="JD016_13425"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAU5000632.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEDILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSNKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5502..5603
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5786..6358
                     /locus_tag="JD016_13430"
     CDS             5786..6358
                     /locus_tag="JD016_13430"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAU5000633.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6422..7072
                     /gene="fsa"
                     /locus_tag="JD016_13435"
     CDS             6422..7072
                     /gene="fsa"
                     /locus_tag="JD016_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAU5000634.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7209..10148
                     /locus_tag="JD016_13440"
     CDS             7209..10148
                     /locus_tag="JD016_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU5000635.1"
                     /translation="MRRRKMDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKK
                     NNILDSRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENL
                     NQSKIGIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGL
                     HTIIYGETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVV
                     KGAYTGADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNR
                     KANVLIISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRV
                     NKNFIITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDY
                     IKSGYYDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLE
                     SKCFSEEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFI
                     KEQFKHVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIV
                     EDLTQVTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGI
                     ECTLGIEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRC
                     KTVNRVDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNV
                     IITLCLSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAI
                     CGTIDIDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNF
                     EGISKEYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKP
                     AIAIARLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKN
                     EIKELLS"
BASE COUNT         4216 a         1004 c         1936 g         3123 t
ORIGIN      
        1 aatcatatac tccaaaatac tacataaata aaattaaaca aaaactaaaa tgacaaatgt
       61 cattatctaa agtgacatag aaacggtttc ttaaagttgt tactataaaa tataaactct
      121 ataataaaag tataacaaaa gggaatcata tcgatataga atataattca agaggaggta
      181 aaattatgag tagttctaat caatcgacaa taaagacatc tatacagcga gtagggaaat
      241 ttttaagtgg tatggtaatg ccaaatatag gagcttttat agcatggggt cttataacag
      301 cattatttat accaacagga tggatgccta atgaaaaatt gagtactata ggtgacccaa
      361 tgataaaata ccttttacca ctattgatag cttatactgg tggtaaagca atagcaggac
      421 aaagaggtgg cgtaataggt gcagcagcag ctatgggagt aattgttggt gcagatatac
      481 caatgtttat cggagcaatg ataatgggac catttgcagg atgggtaatt aagaagtttg
      541 ataaatttgt agatggaaag ataccaacag gatttgaaat gttagtaaat aacttttcaa
      601 taggtataat aggtatgctt cttgcaatac taggatttta tgcaattgga ccagcaatag
      661 tagcaggaac agctcttata gaatctggag ttcaatttat agttagcaaa agtttattgc
      721 ctctagtttc agtatttata gaacctggaa aagtattatt cttaaacaac gcaataaatc
      781 atggtatatt agggcctata ggtatagctg aggcaaaaga agcaggtaaa tctataatgt
      841 tcttactaga aagtaaccct ggaccaggtt taggagttct attagcatat tggatgttta
      901 gtagaggaag tgtaaaacaa tcagcaccag gagctgtaat aatacatttc tttggtggta
      961 tccatgaaat ttacttccca tatattttaa tgaatccagt tttaatatta gctacaatgg
     1021 caggtggagc agcaggaata ttgacttttt caatattagg agctgggctt gtagcagcac
     1081 catcaccagg aagtatattt gcacttatgg cactagctcc aaagggagga ttacttccag
     1141 tattagcagg agttgcagtg gcaacagttg tatcattctt agtggcagca ccatttgtta
     1201 agagagcttc agcaaatcaa agtgaagaag acagtacttc tttagaagaa gctaaagcaa
     1261 aaatgagtga tatgaaatct gcaagtaaga actctgagaa aaatattgag gaaaaacagc
     1321 ttgaagtaaa tgagattaag aagattgtat ttgcatgtga tgcaggaatg ggttcgagtg
     1381 caatgggagc ttccagattt aaaaatagaa taaaaaactt agacttaaat atagaaatta
     1441 caaattcatc tgttgataat cttccagatg atacacaaat agtagtaact cacaatacat
     1501 tagttgaaag agttgctaag aataattcga gtgttgagat agtttcaata aacaattttt
     1561 taaatgaccc aaatttagat acattattta aaagattaga atctaaataa aaataaaaag
     1621 taccactaat taggagtgga taatttgaaa aaaaagaaga ttacatctag gcaaaaaaag
     1681 ataatactaa tgatagtaga aaattcaaag aaaaacattc caataactat ttctgagatt
     1741 gcagggactt tagagttaag ttcaagaact gtacttagag atatgtctgg aattgaaaaa
     1801 tggtttgatg aaaatgattt taactttgta aaaaaaccag gagtgggatt gattttagag
     1861 gaaaatatag aaaatcaaaa ctttataatt gagttgttag aagaagaaaa aatagaaaaa
     1921 gaatacaaca aagaagaaag aaatttaatt atattatcaa agctactagt ttctaacgag
     1981 ccagtaaaat cctattattt tgcaaaaata ttaaaggtat ctgaaggggt acttaataat
     2041 gattttgcct tagcaagtaa atggttggaa agatttgata ttgagcttgt tagaaaacca
     2101 gggcttggtg tctacttaaa aggtcaagaa aaaaacttta gagaggcata tgtaaatctt
     2161 atatacgatt cattcaatga aaaagaaata ctagatatgg tcagaaatat aagtgaaaat
     2221 atacaaacag ataaagcaat agaaatttta agtgaaaata gacttttaaa tttaatggac
     2281 aggtgcataa taagaaaagt tgaagctaca ttaacaaaga agctttcaga tttagatgta
     2341 aatttagctg atagtgcata tatagggctt gtagttcata tttcattagc cttacagagg
     2401 ataaaaaatg gtgaaaatat aactatggat aaagagtttc taaaagaatt atctataaca
     2461 gaagagttta aattagctaa agaaatcgta aaaggtatgg aaacagattt taacatggat
     2521 ataccagtag atgaggttgg atatataact atgcatataa gaggtgcaaa gcaaaggtca
     2581 tcttcaaacc ataaggcttt aaacttagat gatatagaaa taatggaaat tacaaataag
     2641 atgatagatt tggcggaaga tgaatttaaa ataagtttaa aaaatgacga gaggttattt
     2701 aaagacctag caaatcactt agggccttct ataaataggt tgaatatggg attagaaata
     2761 agaaatcctc ttttagatga aataaaaagc aagtattcat atgcctatga tggagttgaa
     2821 aagatttcta gaattataaa agataaacta aatataaatt caatacctga atcagaaata
     2881 ggatatattg caatgcactt tgcatcagcc atagaaaaaa atcttatgat gaatactaat
     2941 ataaatattg ttgtggcatg tcccactgga ataggtactt caagattctt atcaactaag
     3001 attgaaaata aatttcctaa tttaaatata cttgaaacca tatcagctat aaacatagat
     3061 gaagagtatt taaaggaaaa agatgttgat ttaatagtgt ctacagttga gttaaataca
     3121 agtttaaatt atatatgtgt aggtccattt atgagtttag atgatgaaca gataatcaag
     3181 gaaaaaataa aatctatagc tcaaaataaa ttaatcaatt taaatgcgaa aaatgatact
     3241 aagagtaaga ataaagtata tgaacagata acagaaagta tgaatatagg aaaagatatt
     3301 cttcaatttt tagaggaaat tagatttgaa aaatttgaaa gtaaagattt aagtgagctt
     3361 attgaagact catcaaaaat atttgcaaaa tctagtgaag atataatctc tataaaagaa
     3421 tcgttaaaag aaagattaaa aatttcaatt ccatatatag aagaatcaaa aattctattg
     3481 ttacattgta tgagtgaaag aatagatatt atgaagttat caataataaa actagagaat
     3541 agaatcgtgc tagattcaaa tgaagaaatc gacaatatag tttttatgtt acttcctaaa
     3601 aattcaccaa gttatcaaag acaaataatg agtgaaataa gtggttcatt aatagacaat
     3661 ataattttta ctaataagat aaataaattc tctatagaag agatgacatt agaggtaaaa
     3721 gatatagttt ttaattttta tacaaacaga ttaaaagcat ttatagataa ataaaattaa
     3781 tattaaaaag gaaggttgat actatgagta aagtattgaa tgagaataat atatttttag
     3841 ggttagatag tgtttcaaag gaagaagcta taactttagc aggaagaaaa ttagttgaaa
     3901 atggatatgt aaaagaagaa tatataccag caatgttaga aagagaaaaa gtgatgacta
     3961 catatatggg aatgggagtt gctattccac atggtgtaaa tgaagcaaaa aaagaaatat
     4021 tatcttctgg aatagttata ttacaattcc ctaatggtat agattttgat ggagaaaaag
     4081 catatttatt aataggaata gctggtgttg gagatgaaca tttagaaata ctatctaata
     4141 tagcagtagt tttagatgat gacttaacag aaagactaaa aaattcaaat gacaaacaag
     4201 cttttatgga agcttttgcc aactaatctt aggagggatt taaaatgaaa aaggcaattc
     4261 agtttggagc aggaaatatt ggaagaggtt ttattggagg cttgttagtt aagtcgggtt
     4321 atcatgtagt atttgctgat gtaaatgagg atatattaaa ctcaataaac aaagataaaa
     4381 agtatactat acacataaga gatgtagaat gtatagatga agtaattgat aatataagtg
     4441 cggtttcatc tataaaagaa gaaataatag atgagattgt acaggctgaa ataataacta
     4501 cagcagttgg acctttagtt ttgactaaaa tagcctctac aattgcaaaa ggtataaagg
     4561 ctagaaaaga aaagggatta acaagtaatc taaatataat agcatgtgaa aatgcaatat
     4621 acgcaagttc ttctttaaaa gaagaggtac ttaagtattt aaataaggaa gaagtagaat
     4681 atttagagat gtatgttggt tttccaaact gttcagttga tagaatagtt cctcctggta
     4741 aaaacgaaaa tcctcttgat gtaacagttg aaaattttta tgagtggaat gttgaaaaac
     4801 aaggatttaa aggggaaata ccaactatag ttggaatgaa tttagctgat aatcttatgg
     4861 cttatataga acgtaaattg tttacactga atacaggtca tgctataaca gcttatatag
     4921 gatatttaaa aggatataag actatagaag agagtataaa agataaattt atctgtgata
     4981 tagtcaaaag tgcaatggta gaaagtggag aaggattgat taaaaaatac aattttgact
     5041 cagaagtgca ctataaatat attgataaaa ttttaaatag atttaaaaat ccatatctta
     5101 atgatgatgt attaagagtc ggaagggaac cattaagaaa actaagtaat aaagatagat
     5161 tgattaaacc tttaatgaca gcaaaatcat atggattatc agttgataat ttgattcttg
     5221 gaataggtgc agcgcttcat tataataata gtgaagatac tcaaagtgta gaattacaag
     5281 agttgataaa gtctattggg gtaaaaaaag ccgttgctaa aatagctaat ataagcaatg
     5341 acgaagaact tttaaataat atcgaaaaaa gttatatatt tatgaaaaat ttataaattt
     5401 acttgaaaaa aagtagttct gataaagtaa ctataatata ttattctaca cagtttgaat
     5461 tatttgttga ataatagaga attataatat ataattaaaa ggaataaatt aatatattta
     5521 cctgtatatg tttgataata tggttcaaat gtttctacta agctaccatt aatagcttga
     5581 ctacaggagt gaagaagata tagtttatac ttattttaaa ttatatatta agttaattac
     5641 tttgaatttt aagctcctgt ctatgatata gacaggagct tttttagtat tttaatataa
     5701 aatacttaca agaatagata gcaaaattag aacaaatata atttttttat taatataatt
     5761 ttagaaaggg ctaggtggaa gttcaatgaa ggctttacaa gaaaagatat tgagagaagg
     5821 cagtgtttca ggaaatgata tactaaaagt tgatagtttt ttaaaccatc aaattgatgt
     5881 agctttttta aatgaaatag gaagagaatt taaagagaga tttaaaggag aaaaggtgga
     5941 taagatattt acaatagagg cttcaggaat agctatagca tcaattgttt cacagtattt
     6001 tgataatgca cctgtagtat ttgcaaaaaa atcagaatct aaaaatttgg ataaagatgt
     6061 atatgaaact aatgtatatt catttactaa ggcaagagag tattcagtta aagtttctaa
     6121 aaagtacata aacaaaggcg aaaatatatt gatagtggat gattttttgg caaatggaag
     6181 agctgctctt ggacttaaag atttaattga gcaagcagaa gctaacttag ttggagtagg
     6241 aattgtcatt gaaaaaggat ttcaagctgg aggagcactt ttaaaggcta atgatgtaag
     6301 attagagtct ttggcagtag ttgaatctat agataatgga actgtaaaat ttaggtaatt
     6361 tatgatatta tatacttgtg tttagcacat attttaacta tgtaattatg gaggattttt
     6421 tatgagattt tttatagaca cagccaatat tgaagaaata aaagaagcaa atgatttagg
     6481 tgtaatctgt ggtgtaacta ctaatccgtc tctaatagct aaagaaggta gagattttat
     6541 agaggtagtt aaagaaataa gtgaaatagt agatggacca ataagtgcag aagtaataag
     6601 tttagaacat aaaggtatga tagaggaagc agaaaaatta tctaagatac ataaaaatat
     6661 agttataaag atacctatga cagcagaagg tctaaaagcg gtgaaagtac tatcaagtaa
     6721 aggtataaag actaatgtaa cgcttatatt ctctgctgga caagctcttt tagctgctag
     6781 agctggagct acatatgtaa gtccttttgt tggtagattg gatgatatat cacaaaatgg
     6841 tcttgattta attgaagaaa tcgttgatat atttagtgta aatagtattg aggctcaaat
     6901 aatagttgct agtgttagaa atccaataca tgttttacaa gctgctagaa tgggagcaga
     6961 tatagcaaca gttcctctta aagttataaa ccaaatgatt aagcatcctt tgacagacaa
     7021 aggaatagat agctttatga aggattggga aggtgcttct ttaaaattat aattataaac
     7081 tacgctttat aagtatatta aagaggttat tatggaaatt ctaagtaata aggctaggct
     7141 attttgccta gtcttatttt ggtgtataat atattgaaac atattattga aatagattaa
     7201 ttttttaagt gaggaggcga aaaatggata aaatattaag agagatacaa agagaagata
     7261 aaaaaaatcc atatactgac caagaacttg ctgaaatgtt aaatgtagct aggtctgaag
     7321 tgatatcagt taggaaaaaa aataatattt tagattcgag agaaagaaga aaaaaaatat
     7381 taataaaaga tatatcaaaa attgtaagtg aaaatcctca aatgtctgaa agaaaaataa
     7441 ctgaaatatt aatagaaaat ggatataata tatcgagaag tgcagtttca aaattattaa
     7501 aagaggaaaa tttaaatcag tcaaagattg ggattaagga taaagaccca gcatcatcag
     7561 gaaagtatgt gaaggtgagt aaagttgtag aagaagatgg atttgaagaa ttgataggta
     7621 taaagggaag tttgaaagaa aaggtaaatc tagctaagtc agcaataatg tatcctccta
     7681 atggccttca tactataata tatggagaaa caggagtagg taaaagtgaa ttagctacat
     7741 gtatgtataa atatgctata aaaaacaaca ttaaagaaga gaacagccca tttatagtgt
     7801 ttaactgtgc agactatgca gaaaatccta atctattgat agctcaactc tttggggtag
     7861 taaaaggtgc atatacagga gcagatgcaa atagagaagg tcttgtagaa caagctaata
     7921 atggaatatt gtttttagat gaaattcata gattgcctgc tgctgggcaa gaaatcttat
     7981 tttcattaat agatagagga gaatttagaa ggttaggaga aagtagttct aatagaaagg
     8041 caaatgttct cataataagt gctacaacag aaaatccaga ttctaattta cttcaaacat
     8101 ttagaagacg tataccaatg gtaataaatt tacctaatat gagtgaaaga ccaaaatctg
     8161 aaagatatga aataataata aagttttttg aaagagaagc aaagcgagta aacaagaatt
     8221 ttataataac taaagaagtt atgtgtgcac ttatgaatta taagtgtact ggaaatatag
     8281 gtcagttaaa gagtgatata caagtaactt gtgctagagc ttttagtaaa tcgatatttt
     8341 ctagcgataa agtaattata gatttagata gtttaaggga ttatataaaa agtggatatt
     8401 atgattgtaa ttgtaaaaat gaaaagtatt ctgagttttc agttgaggat atatatatag
     8461 atattagtga attggtgagt ggaaaaaata gaattttatc tgataatgag ataagtgaga
     8521 tatataatta tgcagaaaaa gagcttaagg ttttggaaag taagtgcttt tcagaagaag
     8581 agttaaaaaa tgcatttata gaaaagttag ataagaagtt taatgctatt aaaaacaata
     8641 aaaatttatc tgatagaaga agtcgaatag actggggagg acaattaaaa gagtctacac
     8701 tggagataat ggataaggta attgtattta ttaaggagca gtttaagcat gtaaatcaag
     8761 gtctgtactt ggctctagca attcatattg agcatgctat aggtaggata aaagatggaa
     8821 aaactataat aaatccaagt ttagataaaa taaaagcatc aatgccaatg gaatatgagt
     8881 tgtcaagata tataatgggt atagtagaag atttgacaca agtaacattt ccagaagatg
     8941 agcttggata tttggcttat tatataaata aattttgtta taatgaggaa agtataaaag
     9001 ataaagttaa agtagtaata gttacgcatg gtaaagttgg aattgaaatg tctaaagttg
     9061 taaatcatat tttaggtata gaatgtacac taggtataga aatagcactt acagattcac
     9121 catcagaggg gatagagcat gtattagaag agcttcaaaa gatagaagct agaaaaggta
     9181 ttctagttct aattgatatg ggttctttgg taattcttgg tgatgaggtt gaaaaaaggc
     9241 ttggaattag atgtaaaact gtaaatagag tagatacatt attggcaatg gaagcaggaa
     9301 aacttgcaac cattgaaggg aaaagtttag atggtataat agctgattta aagaaaaata
     9361 agaattatgc tatgataaat acaaataaat ttagttacag aaaaaacgag tatggaaaga
     9421 aaaatgtaat cattacttta tgtttgagtg gtgtaggtac agctttaaat ttaaaagagc
     9481 atatagaaaa acaaattaag gaatatgaca ctttaattga agtaaagcca gttgcttttt
     9541 taaataataa tttagaagaa gagttaaatg atattgaagc tgaatataat attattgcta
     9601 tctgtggaac tattgatata gattataaaa acgtgccttt tatttcttat aatgaggtac
     9661 ttggtaaaaa tggtattaat aaagtattag agcatttaaa tagtaaacaa ccagctttaa
     9721 atattgaaaa ccagttgaat aatttaatac atgaagattt gattttatac aattttgaag
     9781 gaatttcaaa ggagtatata attgatactc ttgtttcaaa acttgaagaa ggtggatatg
     9841 tcgactcaaa gtatatatta agtgtatata aaagagaagc aatgggaagt gttgtaatgg
     9901 cctctaaggt agccgtgcca catggacttc cagaaaacgt tataaagcct gcaatagcaa
     9961 ttgcgaggtt gaataaacct atagtatggg acaataaatt tatggtggat ttggtggtac
    10021 tactggcact taaagaaaat aataagaaag aaatacgaag cttattttca aagattaatg
    10081 accagcatac tttagaagtt ttattaaata ctgaagataa aaatgaaata aaagaacttt
    10141 taagctaatt gttagtaccg agaagaaaga caaaaaaatt tgctttagta taaaatttaa
    10201 aactactcaa ttgctaagta gttaattact ctatataagg ctagttctat gaaatttatt
    10261 tatatgattt tatagaatt
//
LOCUS       DACXMZ010000144        10187 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.145, whole genome shotgun sequence.
ACCESSION   DACXMZ010000144 DACXMZ010000000
VERSION     DACXMZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10187
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.145"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..867
                     /locus_tag="JD016_13445"
     CDS             <1..867
                     /locus_tag="JD016_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAU5000636.1"
                     /translation="KYKYILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIE
                     NENLSKLYSQNLYDKLRISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVG
                     HSLSTTIIQLNAIEKLLKDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQS
                     LFKIKELAKSFIKLTNINVKLTISKNTWNLSRNQSITLYRLIQESLSNSSKHGKATEI
                     RIFITFNTSNLIITISDNGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRA
                     SFPKFSGGDFFE"
     gene            860..1507
                     /locus_tag="JD016_13450"
     CDS             860..1507
                     /locus_tag="JD016_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000637.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1636..2571
                     /locus_tag="JD016_13455"
     CDS             1636..2571
                     /locus_tag="JD016_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000638.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPKSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2585..3682
                     /locus_tag="JD016_13460"
     CDS             2585..3682
                     /locus_tag="JD016_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000639.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQNSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3685..4749
                     /locus_tag="JD016_13465"
     CDS             3685..4749
                     /locus_tag="JD016_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000640.1"
                     /translation="MIRLTIANIKRYLKNHNLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            4993..5490
                     /locus_tag="JD016_13470"
     CDS             4993..5490
                     /locus_tag="JD016_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HAU5000641.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNIN"
     gene            complement(5666..5914)
                     /locus_tag="JD016_13475"
     CDS             complement(5666..5914)
                     /locus_tag="JD016_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000642.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(5980..6996)
                     /locus_tag="JD016_13480"
     CDS             complement(5980..6996)
                     /locus_tag="JD016_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HAU5000643.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7054..7821)
                     /locus_tag="JD016_13485"
     CDS             complement(7054..7821)
                     /locus_tag="JD016_13485"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAU5000644.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYESIEKYGITPIHRKSFLKNIL"
     gene            complement(7889..10078)
                     /locus_tag="JD016_13490"
     CDS             complement(7889..10078)
                     /locus_tag="JD016_13490"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HAU5000645.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3546 a         1565 c         1183 g         3893 t
ORIGIN      
        1 aaatataagt acatattgtt ctcttttgtt ataatatctt ctcttattat tagtttagat
       61 aaaaatatat atttggcact ggagtcaagc tctatattgc taattataac tgtactttct
      121 atatatatag aaaatgagaa tttatcaaaa ttatacagtc aaaatcttta tgataagctt
      181 agaatttcta aagatgaatt aaaaaaagtt aatgctgatt tggaaactta tgcaagttct
      241 atagaagaat tagcaatttt aaaagaaaga aatcgaatat caagagaaat acatgatagt
      301 gtaggacatt ctctttctac aacaataata caattaaatg ctatagaaaa actcttaaaa
      361 gacaaacctc taatttatga cttagtacat gaacttagag aatttgtaac tgaaagtttt
      421 caagatgtaa gaagggctat atctgagtta aaacctgttg aatatgaaaa ttaccaaagt
      481 ttatttaaaa tcaaagaatt agctaaaagc tttattaagc ttacaaatat taatgttaaa
      541 ttaactattt caaaaaatac ttggaactta tccagaaatc aatctataac tttatataga
      601 cttatacagg aatccctttc caactcatct aagcatggaa aggcaactga aatcagaatt
      661 tttattacat tcaatacttc aaatcttata attactatta gtgataatgg tataggatgt
      721 ggcaatataa aaaaaggaaa tggacttaat agtatcaatg agagaatata tgaattaaat
      781 ggtaaagttg aatttgataa ttctaataat ggtttcacaa ttagagcttc ttttcctaaa
      841 ttttcagggg gtgatttttt tgaataaaat taaagttctt atagtagatg acgaaaaatt
      901 aattagaaaa ggtcttaaaa ttatattatc atcttataat gatttagaaa ttgtaggaga
      961 tgcttctaat ggatatgaag ctttagaatt ttgtaaaatg aatgatgtag acatagttct
     1021 tatggatata cgaatgaaag tatgtgatgg ggttctaggt acaaggctta taaaggaata
     1081 taataattct ataacattat taattttaac aacttttaat gatgatgaat atataaaaga
     1141 tgctatgaag tttggtgctt caggatacct tttaaaagat agctctgatg aagtattgca
     1201 tgaagggatt cgttcttcat tttttggcaa tattgtgctt gataaaagtg tagctgaaaa
     1261 aatcatgacc tctgaaaaaa cggttaaaca agagcatctt tatgacatgt acaatttaac
     1321 agaaaaagaa atatctataa ttagactaat tgcaaatgga cttaataata aagaaataag
     1381 tcaagaacta ttcttatctg aaggtacaat aaagaataat ataactaata ttttagctaa
     1441 actagaactt cgtgacagaa ctcaactagc aatctttgct ttcaaaaata aaattgtaat
     1501 agaataaatc taaaaatata aagtgactta agtcactttt tttagtgact tatagtaata
     1561 gaattataat tcacaattct ttattataat attatagctt tttaacttat acaaaattat
     1621 tttggaggga ttactatgga tatagtcaga gtaaataata taacaaaaag atttaatgat
     1681 aaattagtac ttgataacat cagcttttct gttaaaaaag gtgagatatt tggacttatt
     1741 ggcccaaatg gtgcaggtaa atctacactt ataaatataa ttacaaatct tatgttacca
     1801 aatagtggta gtattcaaat caatggttta gatttatcaa aagattatat aaaagcaaag
     1861 tctataatag ggttagttcc acaagaatta gcaattatag aaactcttac tccttttgat
     1921 aacttagagt actttggagc tttctacggt ttaaagggaa aactacttaa agaaagaatc
     1981 attgaagcat tagaagttac aggtcttact gaagtcaaaa agaaaaaggt caaaaaatta
     2041 tctggtggta tgcaaaggag attaaatatt ggaatagcac tcttaaatca tcctaaaata
     2101 ttaatattgg atgaaccaac tgtaggtgta gaccccaaat caagaaatca tattttcaat
     2161 tttataaaag atatatctaa aaaacatgaa actactgtaa tatatacttc ccattatatg
     2221 gaagaagttg aacacctttg ttctaaaatt tttattatgg atgaaggtaa agaaattgct
     2281 tttggagata atgattattt aaaatcactc gtatctacaa atacaaaatt aattatggaa
     2341 ataaaaaata taaatgccca acttatcttt gatttaaaaa atactaaagg tgttatatct
     2401 gttttagaaa ataattcatt attagaattg gatatagata aaaaattaca acttacagat
     2461 atactctctg ttattgacaa aaacaattca aaaataatga aaatttctta tgaagaacct
     2521 agcctagaag acgtattttt aaatctaaca ggaaaaaatt taagggacta atggaggtga
     2581 ttttatgaaa ttatacatga gtgtaaaaac tatgctaaag agtttaaaaa gctcctttat
     2641 acttaattta atatattttt tagctttgcc tctgatttta tcttggtttc ttgggatggt
     2701 tacagaaagc atgtttcaaa accctataaa aacagaatct acccccattg ttatatatga
     2761 caaagataac acaagattat caaatgattt gacaaagtat ttgaaaaatg atttatctta
     2821 tatattaaca gttaaaaaag atgattctaa ggcagaatta aagcttacta tacctaaagg
     2881 ctatgagagt agtttattaa atgaaaaatc aaatactcta aacatagaaa aacttggaac
     2941 tcgtgatgat attgcaatat tgcttcaaga tatattagat acttaccatg aaaaatttta
     3001 tttaaacaat tctcagaaaa taagttcaga agatttttct aaattattta ataaaaattc
     3061 tattgatact tctataataa ataacaatgt taaacagaac tcttatgaat actttgcttt
     3121 agtttcacta ggatttcttg taataatttt tataatgaac aatattttgt caaattatat
     3181 aagtgaatca aagggacttt caaaaagact ttattctatg cctataacta gagtacaatt
     3241 cttaatttat gactttgtag gtttatggat ttattcattt atattcttat tattatatgt
     3301 tttattcttt agaatacttg gaataacatt taaaggtaat tttgccatat tgctcctact
     3361 ttgtgcttta tcatcttatt ttatgacttc aatatctacc tttgtaacca gcttttttag
     3421 taaaaaatac ggtacagcaa tagtttatgc attactcttt ttacagacaa tttttggagg
     3481 tatcttcagt atgataagtg atacatttac taagcttaca agtttatctc ctacctattt
     3541 aataggagaa ttgtttagta attatgaaac atttaaaact atagattcaa taggagattt
     3601 aattttcaca tgtttaatta tctctacaat attaattgca cttgctattg taaaggaaaa
     3661 atataaatgg agggaggttt aataatgatt agattaacta ttgcaaatat aaaaagatat
     3721 ctaaaaaatc ataatctttt aattaatatg gttatgcttc ctattatact aattttttca
     3781 cttaattttt ttatcaacaa ctcaggaaat caatcaatgt acttttctcc agtagcaatt
     3841 gtatctgact cttcaggaaa gtatgaacat aagttaataa atagttctaa attaaaagaa
     3901 aattcattta gattaaatga acaagataag gctatgaatc tattaaaaaa taataaggtt
     3961 tctgctgtat tcgttttaga taaaaatttt tcaaatagta tagataaatt acaacgacct
     4021 gtagttaaat gctttaaaat tgaaaatggt ggtggtagtc tttgggcaga atcacaaata
     4081 gaatctttta ttactaaatc gttaaaattg aaagtggata aaaatataga tgataaattg
     4141 acaaagacaa atataattga taacaaacca gataagaata aaggttcatt tttagtagtc
     4201 ttcttgatat gctattttat gtatattaat gcggcacatc ttgcttctga tttatttaca
     4261 ttaaaaaaat caaatgtgct aaaaagactt atctctacag ataataaaga tataaaaata
     4321 attttcagta tatttttagg actatttttt attcaatcaa ttgtatacac ttttgctcta
     4381 ttatgttttt caattataga agactttaat ttgagtctga atgtattgat gattgtacta
     4441 gcaaatagtt ttgtttccac tggatttgta ttttgggtag ctagagtatt taaaaatgaa
     4501 agttctataa gtcttatatc tacattttat tctttaatag gtctagctat atctatctct
     4561 agtctaatac catcaatgga taaattatcc tttatgacta atctttcaaa gttcacacca
     4621 ttttattgga ctatagatgc tattaaaaac aatggtagta ttttaataaa tataatagct
     4681 ctaatattaa ttggaattat atttgtaact gcaggaagtc ttaaactaag agattttgct
     4741 aaaaactaat aattattaaa tataattagt atctattttt atataattgg aaaagaatat
     4801 ttcaacatat taaattattt tatcttatat ttttctcttt agtttcacac accaccaagt
     4861 ctaaacttta tattatatat taatattttt gacttgtcaa gttatttatg ttataatatt
     4921 aatcattaga taattaaagc attgtaccta agttgcaata tctaaaattc aatttggagg
     4981 tataactagc atatgtataa aattggtgaa agtgtaatgt atccaaaaga gggagcctgc
     5041 tctgtaaatg atattgtaac aaagaaaata aatcatgaga tgcaaaaata ctatgaatta
     5101 agtgtcattt ttaatagtaa ccttaaaatt tcgattccag ttctaaatgc agatagaatt
     5161 ggcatacgtc cagttatgga tggaaatgat gttgataatt ttatccaatc tattaataaa
     5221 acagatggtg tatggatttt tgatagaaaa gaaagactta aactgtacca agataaattt
     5281 cattcaggag atgtttttga aattgtaaaa ttaatcaaaa tgcttatgat acaagacagt
     5341 tcaaaacaat tgtgtagcac agataaggag tttttaaata aagctcaaaa atttgcttta
     5401 tcagaattag ctgctgctca atgtaaatcc tatacaatgg tattagagga aatgaaaaaa
     5461 catatattaa attcaaagaa tataaattaa accagttttt taaactggtt ttatttttta
     5521 tactaatcat ccatgctact ttagcttatt ttaaactgcc taatctaata ttaataaagt
     5581 tatctcaatt agatagatgt attttattca aataaaaaaa actaagagta aatcatatta
     5641 ctgatttact cttagctaat aaaatttaat cttcttcaag ttcttttttc tcttgtaata
     5701 atacacttaa ttcatttgct tctaaaactt gtgcctctgc aatatcaagt aactcttgtt
     5761 gccattccac acatttatct ttgtaatcaa aaggtagcgt tctgtcaact atgtacttat
     5821 caatacagtc ctccgcactt ttaattattt cttttctttc atcaataaat ttttctaatt
     5881 ctactaaggc ttcttcataa gcttttctac tcatataaat tccccctttg tacaattatt
     5941 ctacattaaa atgatttttc ctcttttttg tataaaattt tatttgtata taacttttcc
     6001 atcaatatct tttatagttt gacatccagt taatatcata gaactactta gctctgattg
     6061 aagtttattt acataagttt ctacaccatc agttgcacca ccaaaagaag cagtcacaaa
     6121 aggtcttcct attaatactg catctgcacc taaacctatc atttttagta cgtctaaacc
     6181 agttctcact ccaccatctg caagtattgt aactttacct ttaacagcat cagcaatttt
     6241 tggtaaaact tcacatgctc ctggtgtaca gtcaagcact cttcctccat gatttgatac
     6301 aactattgca tccacacctg cttctactgc cattaaagca tcttcaacag tcattatacc
     6361 tttcaatata aatggtagtt tagtagactt tactaactcc tttatttgtt taacattttt
     6421 aggaagtact ggttttccat gtaatgataa agttactaga ccacaggcat ctatatcaac
     6481 tccaactgca aatgcaccag cttcttcagc taatcttatc ttttttataa tattttcatt
     6541 atcccaaggt ttaataaata caattccagc tccattatac tttttgacta catctaaatt
     6601 ttccattaaa aaagcatcta ctgccgtatc accaaccatt gcataaatac cactattagc
     6661 acatcctgca actactggtt ctatgtaatc tctttcatta atttttccac ccatatttaa
     6721 agtagttcct gttactggag ctgcaaatat aggtgcactc attttctttc caaacatctc
     6781 tatagatgta tcaggatttg atacatcatg tattactctc atatttactt ttactttttc
     6841 taaactcttg ctattttcta taaaagaaga accacttcct ttacctccca tgccaggcac
     6901 ttcacctgca caagcaaccc cattacaaac cttacaaact ttacaacttc catttaaatt
     6961 ttctctagca ctttttaata attcattata ttccatcttt aatcccccat taactatagt
     7021 aatttatatt atatacatta tatcatatta tatttacagt atatttttca aaaaactttt
     7081 tctgtggata ggagttatgc catatttttc aatagattca tagtgttctt tagttccata
     7141 tcctttgtga gatttaaacc catattctgg gtatactcta tcatattggt acattatact
     7201 atccctagta actttagcaa gtatacttgc tgctgctatt gatatagatt ttgaatctcc
     7261 tttcacaatt ggattttgag atatgtcaat acctggtatt gttgctgcat caactaataa
     7321 ataatccggt gcttttttta aacaatttat agcctttttc attgccatat atgtagcatt
     7381 taatatatta aattcatcta tttcttcatt gtttactata cctattccat aatctagtgc
     7441 ttcttctttt attacttcaa aaagttcatc tctttttgcc tcactcagtt tcttagagtc
     7501 atttacacct tctattttgg tatctttttt aaatactaca acagaagcta caacaggtcc
     7561 agcaagtggt cctcttcccg cctcatcaat tcctccaata tataaataac ctttatcata
     7621 gccttcgttc tcaaatatat ttatagtttc caatctttct tcttcttttc taatattatc
     7681 cagctttttt gctagtttta tagctagacc ttgtacagat tttctttcat caactcttaa
     7741 cagctctata tattccatat atttttctac ttctaaagtc tctattattt ctttaatctc
     7801 cctcacactt ttatcctgca taccattccc tcatctttcg tttttaaaaa tttaattttt
     7861 aaatatatta aaatttttta attagagttt aattgcatcc actatagcca cattttaaac
     7921 atatactaca tccacctgta tgtgcaattc cttcacttcc acactcagga cacacaaatt
     7981 tttcatcttc ttcaaaattt ttatttatta tttctttggc tttttcatct ttattatctt
     8041 tttctttttt attattttta taatattctt ttttactatt ttctttttta tcttctttat
     8101 tattttcttc ctttttttca tttattaaaa ttccactcct tttacatcca tctctaaata
     8161 ttgttactcc tttaagacca gcctcccatg ccttaatata tatattgaaa acatcttcaa
     8221 ctgtagtttc aaaaggtata tttattgtag atgatataga agcatcaata tacttttgcc
     8281 atactttttg catgtttatt ctttcttcat atcctatatt catagcagta acaaaaaaat
     8341 ctggtaaaca ttcttcatct gtaatacctt tcttgtccat atactcttta acaattggag
     8401 tatatacttt ataatacaca tcctcatcat gcaagctttc agtttttctt atatatgaaa
     8461 gattaaatat aggttctatt ccaccactaa tacctatcat tgtagatata gaacctgtag
     8521 gaggtatggt taaaagttgt gaatttctaa gcccatactt tttaaccatt tccttagtgt
     8581 cttcatttag attttctata aaaaacttag atttagaaat attttcatat ttatactcac
     8641 taaatgttcc atattcttta gcaattagtg ctgattgctt cacagcttca tttaacatag
     8701 tcttaccaat taaatcacat agttctaaag attcatcaga accatatctt attcccattt
     8761 ttataagcat atcagctata cccattacac ctaaacctat ctgtctatac ttatgtacac
     8821 tatctctttg ttcttgtaat ggatgcaagt tcaatccctc atctaagaca tcatttaaag
     8881 ctactacagc ttctctaacg caagatttta atttattaat atcaaaagta gcatgttcac
     8941 caaatggttg tattataaac tctgacaagt ttattgagcc aagtagacaa cttcctccac
     9001 ttggaagtgg ttcttctgca caaggattta ctcctgaaaa gcaaaagttc ttatcttcac
     9061 tcataaaatt atatgttttt attttatccc aaaacaatat acctggttct gcataatccc
     9121 agttattttt cacaaattta ttaaataaat cataagcatt tactatctta actatttttt
     9181 ctccagtagt ttcaacttca aaagtacaat tatataactt tttttctctt acagcttgca
     9241 taaattcatc tgttactctt acagaaatat ttgctttagt aaccttctcc aaatctgttt
     9301 ttatatctat aaagtcttct atctctggat gatttacatc catagaaatc ataagagctc
     9361 ctcttctccc tctttgacca attaagccag ttgtcatact gtaaagttcc ataaaagaga
     9421 ctgctccagt cgtatgtttt gcagcattgt taactttcgc tccttttggt cttagctttg
     9481 aaatatcaat tccaactcct ccaccataac taaaagttct agcaagtttt tttgcagtat
     9541 caaatatatc ttctatgcta tcttttggtg gttctattac ataacaatta gaatatgtta
     9601 ctttttttcc ctctttctgt aatcctctat ttgctaaaat tcttccagca aatagaaatc
     9661 ttttttgtct tattagtttt gatattctgg aactattatt tgaaattctc tttatccatt
     9721 catcaaaact ctcaccatta tgtctatact tgttcttcca tattgatttt tgtaattcat
     9781 caaactccca agctttagtc cttatttcag agcgtctttc tctatataat atgtagtgtt
     9841 ttgctatatc ttttctattt gattccataa gtagtacttc aactatatct tgtatttctt
     9901 ctacggataa gcatttttcc atagaattat agtcattagc tatttttctt gatatatcat
     9961 atgccaactc tctatctact ccaccttcac tatttatact agctttatag acagcatctt
    10021 caatcttact tttactaaaa agttcttttg taccatctct ttttattatg tatttcattt
    10081 ttaaacctcc aataaattag tattaaatta atacacacat taactataac tctaaacaaa
    10141 ctttttatat atttgtatat tttcatatta attatctaac ataatat
//
LOCUS       DACXMZ010000145        10073 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.146, whole genome shotgun sequence.
ACCESSION   DACXMZ010000145 DACXMZ010000000
VERSION     DACXMZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.146"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(27..869)
                     /locus_tag="JD016_13495"
     CDS             complement(27..869)
                     /locus_tag="JD016_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU5000646.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTKIIAYAEANLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1130..3172)
                     /locus_tag="JD016_13500"
     CDS             complement(1130..3172)
                     /locus_tag="JD016_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAU5000647.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLAVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(3324..4295)
                     /locus_tag="JD016_13505"
     CDS             complement(3324..4295)
                     /locus_tag="JD016_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAU5000648.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(4296..5273)
                     /locus_tag="JD016_13510"
     CDS             complement(4296..5273)
                     /locus_tag="JD016_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000649.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            5492..7051
                     /locus_tag="JD016_13515"
     CDS             5492..7051
                     /locus_tag="JD016_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAU5000650.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            7157..8116
                     /locus_tag="JD016_13520"
     CDS             7157..8116
                     /locus_tag="JD016_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000651.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     SKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            8119..8931
                     /locus_tag="JD016_13525"
     CDS             8119..8931
                     /locus_tag="JD016_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000652.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIITSFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(9147..10049)
                     /locus_tag="JD016_13530"
     CDS             complement(9147..10049)
                     /locus_tag="JD016_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAU5000653.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
BASE COUNT         3467 a         1541 c         1251 g         3814 t
ORIGIN      
        1 tacattctgt attaaactac tctttattat aaattcagta cagatataac tcttttgata
       61 tgtaatgtta aataaagtag ttcatcttga cttacatagt aatcataatt cttttctaca
      121 tgagttttta ttttatatgc acaatcataa tccttttcat actttacttt aattatatct
      181 ataatttcat tatttgtatc attgattttt tcttttttta ctaatcgttt tgcaaagaat
      241 tttaaatgag tcagaagtct atcataattt atatcatctt ctttaaattc aactctataa
      301 taatatctta taatatttag tatgtctttt acaattttag taattaatag tgattccttt
      361 gaacttccct tatagtttga atttactata tgcattgcta taaaaccagc ttcatctact
      421 ggaaatttca catcaaattc tttatttata taatctacag cccatttccc aatctcatat
      481 tcttttttat gtattctttt tatctcatgt agtaaatcat tctttatagt tatgttttca
      541 tgatatcttt taattgcaaa tgcaatatgg tcagtaagtg ctacataaat atattcataa
      601 agcttagtat tcaaattcgc ctctgcataa gcaattattt tagtactaac ctcaaaaaat
      661 ttctcatcaa cctggcttgc tagtttacta attttatcta aaaaactttc atcttgtatt
      721 acaaaagttc tttcaatttt acttttatct atttcttgac ctttcttttt ttgaaaccca
      781 attccacatc ctgttaatat taattctttt tttatatttt catctaaaac taatacaaca
      841 ttattattat aaatttgttg aataatcata atcaatatct ctcccaaaat acaatttccc
      901 ttattcataa aaaaatacct aaattaagat acaattcacc tgtatacctt aacctaggta
      961 ttgcctgctt accagtaaca atccagcttg ttaacgctta tatattgtat tcaatttatg
     1021 gttttattct attataaata aatatatatg tcaagtaaat tattgaaaac tatttcctat
     1081 tttttagtat taattttaac atcaaaaatc aaattttatt ccattatatt tatgattcta
     1141 tcatcatcat ttcatcatag agaatatttt ccaataactc tttagccact tctcttgata
     1201 tttgcttagt ttcaactaat tgttgaacac tgtctctttg actttgtatc gccattactc
     1261 tgtagtattc tactcttgcc tcaaacttat catcattttg aatctttcta gtaatccaat
     1321 tcaaataacg attatagaat ctgattgcta ttaaggcctc atgtctgtta tcttcatcag
     1381 tatttttatt tataaatgat atagcagttt tagtagaaag tgcatatgca tggtgaaact
     1441 ctttgattat ttctttattc tcagatacta tttttgtaat tttttctttt acttctttct
     1501 ttacttttct atttataatc cacatgctta tttttaattt tacaatttta aatatagatt
     1561 tttgaacaga ttttcttgat tttgtaatat aaaatctata caatttaaag gctctatcag
     1621 aaattttacc atcttctaat agtttcaaaa cagctaataa tccctgttgg tctgcttctg
     1681 caatcatcat acttataagt ttattatctg gagtatttag tattcctttt tctgagtcta
     1741 ccaactgttc atttaaatca tgtataactg gttgtacttc ttgtgctttt tcattactaa
     1801 gttttaaaat ggcttctttc aaaattagtt tatatgcctc tttatcagat aataaaggtt
     1861 ctgactcttg tacgttattt tttaataatc ttggcagtaa aactgttgcc aatactaaac
     1921 ttataagaat aacttctgat gatataaata aaacagtatc tctcattgga aatacatctt
     1981 tatcagccaa ataaaaaggt atagataagg cagttgctag tgtgaatgta ccatgaacac
     2041 cacaagtagc aactattagt gagtatttac atttagaaat actctctttt acaacttctt
     2101 catccttaaa acctaaaaaa cctacaaaaa aattatttaa aggattcttt ttaggtttta
     2161 taaaagaatt atgccataaa tatacccata taaatcttat aacaaataaa ataagtacaa
     2221 ttaaagtaga aataaaagtc aattccaatg ccatattttc atttttacta ctaagtcctg
     2281 tatatatact tggtaacaaa aaaccaagca gtgtaaagac aaatccattc aatacatact
     2341 ccaaaacata ccaagtattg tctgatatta tccttaattt agtagttgta ttttgtaaat
     2401 gctctttttc aattccatga gctatccctg ccattacaac agctaggatt ccagatactc
     2461 ctatttcttc tgctacaaaa tatacaaaaa gtggagtcat tatttgtata actattacca
     2521 ttggaatttc ttccaaattc cacttgtgta aactaagtct tatttttata attatgtatc
     2581 ctaaaatact tcctactatt attcctccaa aagctgtaat cataaattga atccctgcct
     2641 cttctattga aaaaactcct gtaactgtgg caagtactgc aaccttaaat gctacgactc
     2701 cagcagcatc atttaataaa gattcacctt caagtatact catcatattt ttaggaaaat
     2761 ttaatccttg tgtaattgat tttactgcta ctaaatcagt tggagatatt actgctgcca
     2821 aagcaaatga aacagctaat ggcatcccag gaatgagaaa atgtataaag attccacctg
     2881 caaacacggt tattaaaact aaaccaaatg caagaagcaa aattggtttt ctgagctcta
     2941 atagctcttt tctcgataca ttttgtcctt cactgaatag taatggagct atgatacata
     3001 ttacaaatat ttctggatta aatgaaaatg agaatttaaa tggcaaaata gacaatataa
     3061 tccctagtac aatttgatat attgcaagag gaatattact ccactttttg ctaaatacag
     3121 ttgaaatcgt tgtacaaacc aataatccca acaatgttac taaaattgac ataactacct
     3181 ctcttttcaa taataatatt atagattaaa cttatatatc aactaactta atattaacag
     3241 catgaaatcc catcttaatc aaaaaaattt tcagtttatt ctattattct tattatatac
     3301 atcaaattta atttttgaaa tatctatact agatgacatg caacaaaatg atttcttgat
     3361 atttctttta aaactggaat ctcttttttg catatttcct tacaataagg gcatcttgta
     3421 tgaaacttac atccacttgg tggatttgtt ggagatggta tgtctccttt taatataatt
     3481 ctatttttct taattgtagg gtcttgtatt ggtactgcgc tcataagcgc ttttgtatat
     3541 ggatgttgag aatgtaagta aagttctcct ttagttgcaa tttctattat agaacctaga
     3601 tacataacac aaactctatg tgcaatatga tttactacac tcaaatcatg agatataaat
     3661 aaatatgaaa gtttatattt atcctgtaaa tccataagta aatttataat ttgagattga
     3721 atagaaacat ctagtgcaga aacaggttca tcacaaacta taaattctgg atttaatgct
     3781 aatgctcttg ctatacctat tctctgtctt tgacctccag aaaactcacc tggttttcta
     3841 tttaaatggt acttagaaag accacaactt tctacaacat ttaatatatt atcttttatt
     3901 tcattctttg gcactaaatt atgttctata actgcctcgg atattatttg ttccacattt
     3961 aatcttggat ttaatgaact atatgggtct tgaaatatca tttgcatttt gggtcttaac
     4021 tttctaagtt cttccttctc taacttatat atatctacac cattaaattc aactttacct
     4081 tcagtttttt caattaatct aagaatggtt cttcctgcag ttgattttcc acatcccgat
     4141 tctccaacta tagccaaagt ttctcctttt tttaaatcaa aagaaattcc atctactgcc
     4201 tttacacatt taatttcctt atcaaataat ccagatttaa cttcaaagta ctttttaaga
     4261 ccttctactt ttaataaact ctcttctttt ttcaattata tctcctcctt tttatataac
     4321 caacatgcaa tactatgacc actattcaat tctattaaag gaggtatctg agttctacat
     4381 ttatcacata ctttttcaca tctatcacta aaataacatg aatctggcat tccaattgga
     4441 tttggtactt gtcctggaat cgaatacaat cttttatctt tgttttttcc aagagcaggc
     4501 tttgatttta gaagtccaat agtataagga tgttttggat ttttaaataa ttcaacaacc
     4561 tctgccactt ctataatttt accagcatac atcactaata ctttgtcaca catttctgca
     4621 actactccca aatcatgtgt aatcatcatt atagatgtgt ttaatttttc ttttatattt
     4681 ttcattatat ctaaaatctg agcttgaata gtaacatcta aagctgttgt tggttcatca
     4741 gctataagta attttggatt acatgataaa gccatagcta tcattattcg ttgtctcata
     4801 ccacctgata attcatgtgg atatgaatta tatatttctt cagctcttgg tatttctacc
     4861 agttttatca tatcgattac ctttttctta gcttcttcct ttgataaatc ttgatgtatc
     4921 ataatacctt caatgatttg acttccaaca gtaaacgctg ggtttaaaga tgtcattggt
     4981 tcttgaaaaa tcattgatat ctcattccct cttattccca taagttcatc ttcattcatc
     5041 tttaaaatgt ctttgcctcg aaagattatt tctccacctt ctatttttga tgttggaagt
     5101 aacttcatca aagacatggc tgttacagac ttaccacatc ctgattctcc aactattcca
     5161 atagtttcac cttcatttat attaaaactt acttcattta cactctttac aatcccttta
     5221 tctgtatgaa aataaacttt taaatcttta acctcaatta gtgctttact cattattttc
     5281 tcctctcagc aaaattactt aagataacat acaccttttg attgtatttt ataaaaattt
     5341 aaattacaac attttcttac attttattat ttttttctat taattataat aatataatag
     5401 ttaatttcta tatatattga tttaataatg attactatta tacaataaaa aatatgtgtt
     5461 aaatattatt ttatggaggt tttataagaa tatgaaattt aaaaaattgg catcattaat
     5521 cttagtttca tcattaatgc ttacatttac agcctgtgct tccaatgata aagacaagtc
     5581 tagtggaagc aaatctggtg gaactgttgt aatacctatg gcaagtgacc cagatataat
     5641 caatggagct tttgcaaacg ttaaagaaga ctctcttgca tcaaatatag tttatgctcc
     5701 tctttatact tatgaaaaag gaaatttagt aaattattta gcagaaaaag tagattttaa
     5761 agatagtaag gaattaacta taaaattaaa atccaattta aaatggcatg atggaaagcc
     5821 aataactgct gaagatgtct tatttacatt taatacagtt ttagacgaaa aacaaaattc
     5881 accatcaaga caatacttat tggtgggtga gaaaccagtt aaagtagaaa aaattgatga
     5941 tttaacagtt aagattactt taccaactgc aagtgaatct ttcttatatg gtatatctaa
     6001 aatttctcca ataccaaaac atgtgtttga aggagaatca aatatagcta agtcagaaaa
     6061 aaataataat ccagttggtt ctggtgcatt caagtttaag gaatggaaaa aaggtgaaag
     6121 catagtattt gaaaagaatg cagattattt tggtggagaa cctaaggcag attctatagc
     6181 gctaaaaata attcctaatg aagcatctca agaagcagct ttaaataatg gcgaaatttc
     6241 acttatgaag acatctgctg aaggatatga aaaggcaaaa tcaaactcaa acttacaaac
     6301 ttatacttat agtgaagaaa gattaaacta cattgtattt aaccaaaata tatcaaacat
     6361 ggctaacaaa gaagtaagac aagccttatc atatgcctta aatagaaatg aaatgataga
     6421 atcagcatat ggaaaagaag gttctgtacc tgctaaatct atattagttc cagaagcaga
     6481 cttctataca gaagaaggtg ttgaaggata tgaccaagat acaaataagg ctaaagactt
     6541 attggataaa agtggtgtta aaattgataa attaaaaata ggttataaca caggtagatt
     6601 tgggcataaa aactatgcct tagtagctca acaagagttg aaaaaaattg gtatagaagc
     6661 tgaaatagtt ccttatgaaa gtaaggcatt cttcaatata ctatttagta atagcactga
     6721 atgtgatatg tatgtaaatg gttatgcttg gggattagaa ccaaacccat atagaggtat
     6781 gtttgaaact ggtcaatatt gcaaccaaac taaatattct aatgctgaaa tagatgcttt
     6841 atgggaaaaa ggatttactg agttaaacaa ggaaaaacgt gaagaaatat ataagcaaat
     6901 acaacaggat atatctaagg atgcgcctat ttacactata gattatgaac aaaatttaat
     6961 ggcagctcaa aaaaatctaa aaggaataaa agatgctaaa ccatctcctg caatattatt
     7021 tgaagattgg tctaaactgt atgtagaata attgatgtaa aattttatat tattaattag
     7081 aaaaggagat gtctaaaaat aatattgttt tattatttta aaacgagaca tctcttttaa
     7141 ttaggagaat ttacttatgt taaaattaat attgaataga gtaaaagttt taatacctat
     7201 gttaatctta atatctatat tatcatttgg acttttagaa ttagctcctg gtgacccagc
     7261 agatgcatat ataaatcctt taatgtcttc acaggatatt gaaaatataa gggttaatat
     7321 ggggcttgat aaaccagtat atataagata tttaaactgg ttgaaaaaca ctttaagtgg
     7381 aaacttaggt atttcttata taaaccatat gcccgttaca gaacaaatta tggaaaaaat
     7441 gggaaataca tttattctta tgggtacttc tttgatattt tcaatattgg tagctatacc
     7501 tcttggaata tttttagctg taaataaaaa ttcaataact agtaaagtat catctgtttt
     7561 taattatata ggtgtttcta taccttcttt ttggataggt atgatactca tttcaatttt
     7621 ttcagttaaa cttaacattt tcccatcagg aggtatgcat actattggaa atgatagtat
     7681 tggagattta gtaaaacatt tagttttgcc tgtaataact ttaggtctat ataatactgc
     7741 tatttttaca aattatgtag aagctagtgt aaatgaacaa ttaaaaaaac aatatgttat
     7801 tacagcaaga tcaaaaggat tatcagaaaa agtcatacta tttaaacatg tcttgaaaaa
     7861 ttcactgaca tcattggtta caatcctagg catgagtata caaaaattag tcactggtgc
     7921 atttgttaca gaagtagttt tctcttggcc aggtatggga agattaatga ttgattcaat
     7981 tttttcaaga gattatactg taattatggc aataactatg ttatcagcac tatttttaat
     8041 tttaggtaat ttagttgctg atatactata cttattaatt gaccctaaaa taaaatctag
     8101 caaaggagga ttctagttgt gtttacaagt atcttaaaaa aattaaaaaa agataaatcc
     8161 tttgcttttg catgtattac tcttagcata ataatcatat taattataac ttcttttata
     8221 ttaaatatag actctaatgc aattgatact acttctaaac ttcaagaacc tagcttaaag
     8281 catatctttg gtacagacga acttggtaga gattacttct ctagagctct ttatggtgga
     8341 aggatttcac ttagtgttgg tattttatca atgcttattt ctgtagtatt tggtactgct
     8401 gtaggtgtta ttagtggata tattggaggt aagctagacc aattcttgat gagattagtg
     8461 gatatgctta tgagtatacc aagtttctta gttttgattg tattaaatac ttatctaaca
     8521 cctaaagttt ccactattat actaattatt ggattcttaa gttggatgga agtttcaaga
     8581 attgtacgtg gcgaaacatt aaaaataaag gaaaatgaat actgtctagc tgctaaagct
     8641 actggtatca aaactagaaa tatactatta agacatatag ttttaaactt aaaagagact
     8701 attgtagttg ctggttcgtt aaatattgct aatgctatct taactgaatc tgctcttagt
     8761 tttttaggtc ttggaatcca attgcctctt gcatcatggg gtaatatgct tcaagatgct
     8821 caaagacata tttttgacaa gttatattta gctgtatttc caggattact tatattttta
     8881 acagtcatga gtttgactat aataagcaaa aaattttcta gtaatactta acaaatttta
     8941 tatagaaaat aaaaacagtg attatctaaa gatatacact gtttttattt ttcgtttaaa
     9001 aaaatacata ttttgtatat tataacttta aatactactc taccatgtta tgtaaagtgt
     9061 attattgctc tcaaacatat agatgaaaaa ctaatagttt attataataa aaattgattt
     9121 atttatttgt tattttaata acatttttaa gagttctttt tactaaagaa atctataata
     9181 aatttaaata catgttggtc tacatggcta taacgttctt ttttaggtct acataatata
     9241 actttccatg taataggatg attaaaaggc ttctctataa ttttgctatt ttcaaaaaaa
     9301 tcatgaactg gtgatggtag aattgttata aaatcagaat ttgtggttga tagcaacaaa
     9361 aaatcccatg aaccagacat tatatatctt tttaacttaa cattttcatc agtaaatttt
     9421 tgcattagct tatgatgtat cataaaagta ggattaaata ttgcaagtaa ctgctcattc
     9481 aaatcgttcc aatctatctt atccttctta gcaagtggat gatttatatt taaaaatgct
     9541 gtaagttcat cttcttgaag cacatgttca gttactacac ttttatcaat tttatgtgga
     9601 tgtaacaata ctgcataatc taattcctgt aagataagca ttctatttaa gtcatatgca
     9661 cccttctcta ctatatcaaa ttctatatct ggattatttg caactaactg tgcaactaca
     9721 tctgaaaatg ctattccaag tataagtgga ggtattccta ttctaacttt tcctttaaat
     9781 tgaactgaat cttctcttaa atcttccatc atatttttgt attcatttat gatatgttct
     9841 gcatttacat aaaatctttc tcctgctggt gttaacccat ttaacctgcc tttatacctt
     9901 tcaaataaat atacatcttc actttcttca aaatttttta taatttgact taatgctggt
     9961 tgagatatgt gaagtgtctt agatgctatt gataaattaa aaccagattt cactatttca
    10021 acaaaatatt tcaactgttt tatatccatt ttaaaccccc ttccccaaaa gta
//
LOCUS       DACXMZ010000146        10005 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.147, whole genome shotgun sequence.
ACCESSION   DACXMZ010000146 DACXMZ010000000
VERSION     DACXMZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.147"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            240..905
                     /locus_tag="JD016_13535"
     CDS             240..905
                     /locus_tag="JD016_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAU5000654.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSNELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            927..1859
                     /locus_tag="JD016_13540"
     CDS             927..1859
                     /locus_tag="JD016_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAU5000655.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            1880..2521
                     /gene="pcp"
                     /locus_tag="JD016_13545"
     CDS             1880..2521
                     /gene="pcp"
                     /locus_tag="JD016_13545"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAU5000656.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(2641..2973)
                     /locus_tag="JD016_13550"
     CDS             complement(2641..2973)
                     /locus_tag="JD016_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5000657.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            3238..3858
                     /locus_tag="JD016_13555"
     CDS             3238..3858
                     /locus_tag="JD016_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAU5000658.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(4173..4997)
                     /locus_tag="JD016_13560"
     CDS             complement(4173..4997)
                     /locus_tag="JD016_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HAU5000659.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(5008..6411)
                     /locus_tag="JD016_13565"
     CDS             complement(5008..6411)
                     /locus_tag="JD016_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU5000660.1"
                     /translation="MKWKITRNFIFIIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRNIIIDI
                     LNDDRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(6415..7140)
                     /locus_tag="JD016_13570"
     CDS             complement(6415..7140)
                     /locus_tag="JD016_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000661.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(7252..8025)
                     /locus_tag="JD016_13575"
     CDS             complement(7252..8025)
                     /locus_tag="JD016_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000662.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(8031..8918)
                     /locus_tag="JD016_13580"
     CDS             complement(8031..8918)
                     /locus_tag="JD016_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000663.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDINSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(8932..9954)
                     /locus_tag="JD016_13585"
     CDS             complement(8932..9954)
                     /locus_tag="JD016_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000664.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
BASE COUNT         3568 a         1536 c         1151 g         3750 t
ORIGIN      
        1 ttgacatata cttaaactat agacttacaa aatcaattaa tattagctta aaatataatc
       61 tttgttattc aaattcctat acacaaccat tatttacttt gatactttaa aacatcttcc
      121 ctatttttta ttaaatttta taataatagt cctattaacg caaatactat aaaagagttt
      181 aactttatcc attaaataaa tttaaattaa aacatataga aatggaagga gaacaattta
      241 tgataaaact aatcggtata ttgatagttg taattggatt tatacttaaa attgacacac
      301 tatttactgt attattagca ggagtggcaa caggaattgt tgcaggatta gattttaatc
      361 aaatttttac aattttagga gattcttttg tctcaaacag aggtgtatca ttattcatat
      421 taacattgcc agtaatcggt gtattagaaa gatatggttt aaaacaaaga gctgtttctt
      481 taatagaaaa attaaaaaga cttacaacag gaaaagtact tactatatac atgatagcta
      541 gacaaatagc tggtgctcta tcaataagaa tgagtggaca tcctcaattt gtaagaccac
      601 ttgttaatcc tatggcacaa gctgctggtt taagtaatag taatgaacta aaagaatcgg
      661 atgaagaagc cataaaggca ttatctgctg catcagaaaa ctatggaaat ttttatggtc
      721 aaaacttatt tgcaggtagt agtggagtat tacttatagc atctactcta actcaatttg
      781 gatacaatgt aactggactt aatattgtaa aggctagtac tattatggca gtaatagcac
      841 ttgtagtggc tacaatccag tttaccttat atgataaaaa attaaacaaa agtcataaaa
      901 aatagtttta tttaggagga ataaaaatgc aaaattttat aaacatatca ttggaaatct
      961 tttacgcact aatgggattt ttaatgattg ttatagcata taagtctttt actaccataa
     1021 ataacaataa gaaatatggt acatcattat tttggatact tatatcactg ccattcatat
     1081 ttggtagatt gattccagcc aatataatag gaataatatt aatattatct agtttattaa
     1141 cactttctaa acaagttgta tttgcaaagt atgaagaacc agatgaaaat tttggaaaag
     1201 aacaagcaga caaacttaaa aataaaatat ttataccatc attaatatta gcatttgctg
     1261 cagtagtagt agctatgagc ctttctaatt ttgataattc ttctcaattt gcaataggtg
     1321 tgggttcaat aattgcatta atatcagcgc ttataataac taaggcaaaa cctgctacat
     1381 cagttcaaga tggctcaaga ttacttcaac aaatgggacc tgctagtatg ttaccacaac
     1441 ttttagttgc attgggagct ttatttactc aagccggagt cggtgaagtt atttcaacca
     1501 tgatatctgg tgtagtccct gctgatagca gattatttgg tgttatagct tatgtattag
     1561 gtatggttat atttaccatg ataatgggaa atgcatttgc cgcattttca gtcataacgg
     1621 caggaattgg tattcctttt gtattgtctc aaggtggaaa tcctgctata attggagcat
     1681 tagcattaac agcaggatat tgtggaactc tacttactcc aatggctgct aattttaata
     1741 tagttccagc agccttgcta gaatgtaaaa atgattatat tgttataaaa tatcaagctc
     1801 cagttgcttt ggtattaatt atagcccaca tattagtaat gtacttttta ggattttaat
     1861 ataattagga ggtaaaataa tgaaaatttt gttaacagga tttgacccat ttggcggaga
     1921 accaataaac ccagctcaag aagcagttga aagggtcaat aataatataa acggtgcaga
     1981 aatcataaag ataactatac caacagttat gacaaaatct gttgaagcta tagataaagc
     2041 tattcaagag cacaatccag atatagtaat atcagttgga caagctggag gtagatttga
     2101 cattacacct gagagagttg ctataaatat agatgatttt agaataaaag ataatgaggg
     2161 taaccaggtt atagatacta ttataaaaga agatggtgaa cctgcatatt tttcaaaatt
     2221 gcctgtaaaa gctatggtaa aacatatgaa tgaaaataaa attccagcta gtgtttcaaa
     2281 cactgcagga acatttgttt gcaatcatgt gatgtatgga attctttata tgatagacaa
     2341 gaaatatcct aatataaggg gtggatttat acacatccca tatacaactt cacaagtaat
     2401 agataaaaaa aatacaccat tcatgtcttt agaagaaata gtaaaaggct tagaattagc
     2461 tatagaagct tgtattatat ataaagaaga tgtaaaagaa attggtggtg aaatttcata
     2521 ataaattttt tagtattttt ataaattaaa aatactaact attaatttaa atatagactg
     2581 tgagtagatt attcaaacaa ttttactcac agtcttttta tggtatctac agatacaaaa
     2641 ttaaggtcta ttcctctctc cccactcaca cattgcatct aaaattggaa tcagagattt
     2701 tcccctttct gatagactat attctacctt aggaggtatt tgaggatatt cagtacgaat
     2761 aatcaagtta tctgcttcta attcttttaa tgataaactc aaagttttat aggagatagt
     2821 atttatataa cgttttaatg cattatatct tacaacttca aattctacaa gacagtaaag
     2881 aattatcatc ttatatttac cactaatcag tgaaagtgta taactaaatc ctgtttcact
     2941 taaatctgta tttgatatac agtctatact catatattac actctccttt tggttagtat
     3001 tgaacaaaaa tgtgcgtact tgtttttaga ataatttata ataaaattat agtgtaagaa
     3061 taattgaaag tcaattttat aataggaggt gtatttacta aaaaaaagtt attttaaata
     3121 ggattctaag attagacatt tctatataaa ttgataatat tcatgaaaat cctattttat
     3181 aaaaaataat atctcttaga aaatctatta aataaaattc attggaggaa atttataatg
     3241 aaagttttat tgataaacgg tagcccaaac caatatggat gtacttatac agcactaaat
     3301 gagattacta aagtattgag taaacacgat attaaaacag aaatactgta tttaggtaaa
     3361 gaaacaatcc caggatgtat tagttgcgca tcctgttttg aaactggaaa atgtattcga
     3421 aatgataaag ttaatgaatt gattgaagat ctggataata ttgatggtat tattattggt
     3481 tctcctgttt acttttctag tgcaactggt caattaactt cttttcttga ccgtctattt
     3541 ttcatagctg ggagtcgcat ggctacaaaa ttaggtgctt cagttgtgtc atgtagacgt
     3601 ggtggtgcaa gtgcaacatt tgaccaacta aataaatatt tttcaatcag caatatgcct
     3661 attgtatcat cacagtattg gaaccaggta catggcttta ctccagagga tgttatgaag
     3721 gatgaagaag gattacagac tatgcgaact ctaggagaaa acatggcatg gcttcttaag
     3781 tgtatatcag caggtaaaaa agctggaatt aaagaaccac aatatgaaga aacaattatg
     3841 actaatttta taaaataaat aaaaaatgta ctaatattaa ttaaaacaaa acactaattt
     3901 ataaataaaa taagcataaa tgtaggataa aaagcactaa aacagaggta ctgaatttta
     3961 tatcaaacct ctgttttatt attacattaa taaccaaacc atgtataaca tcatatattt
     4021 ctaaataaat tatactaatg ttattgaaat tacaataact gaatttctgc tcataaattt
     4081 aaatattcta caaatctcat ctaaataaca attttaatag acctaaaaca atcctcatat
     4141 tgttcatttt gtattaaaaa tatcacttac aatcaaaaac catttattaa gtatattcct
     4201 aagatataca atatatatac atggattgga tagtataaat aaaataaata cttcatactc
     4261 tttccttttt ctccattgta aagtaacatt aaaggtagag aagcaatcat catccattgg
     4321 taattttgta aaatagaatt ttttaaacta taatctccat ttgacattgt tgtaaatata
     4381 aatatacaca ttaaaacata acaaatactc aaactgaact ttttacctct acacaaataa
     4441 aaaataatcc caagtataat ccaaattggt ccaccttcta ctaaaaatgg tactggtacc
     4501 aaaatcataa ataaagcaat aactctgtta gtgactttaa aaagtaaagc tactgtaaat
     4561 attcctaaaa ttataggaat gattatcatc aataatccta taataaaata tttaatttct
     4621 ttctcttttc taaatttctt tatgaactct attgcttgta aataaatcgc tattaaaaat
     4681 aatgttgaaa aaatgttatt tataattata gctccttctg gatgtgaaaa tacattatta
     4741 attatttgat ttcctatagc cataataaca gaccaaatat acaatctact tatatatttt
     4801 tttctgttac tagtgtgata aaatccttca acagccatga atataaacag tggtgctgat
     4861 attctgccta cccaatgaaa ccaaattggt atttgcattg tttgtggcat aaatgctgct
     4921 atatggtcaa atgtcataaa tattaatgct aaaatcttaa tagtaaaccc acttatacct
     4981 tttttaaaaa aatccatatc atatctccta attttgtttt atctctataa taatttttgt
     5041 tcctattcca atttcactac ttacataaat tttaccttta tgaatctcta ttatttcttt
     5101 agaaatagcc ataccaagac ctgaaccttt atgcatctca cctgtattag tacctctata
     5161 atatttttta aaaatatgtt tcaaatcact tttgcttatt ccaattccat tatcttttat
     5221 tataatttct atattatctt tttttacaat ttctacactt atcaaagttc cttcgctatt
     5281 atgaactatt gaattaaata taagattggt tatagccctt ctgaataaga ttgaatctac
     5341 atatacttca attaaatctt cattactttc aaattcaata ttcctatttt tatatctatc
     5401 atcatttaaa atatcaatta ttatatttct gactaaactg actaaattta tttttttctt
     5461 gtccagaact atagtattat tttttaatcg agtagataaa tttaaatcat caactaaatc
     5521 ttttatatac ttagacttat tatatataat ttctgtatac tctcgtattt catcttcttc
     5581 aaactcataa tctttgtcat taattatctc tgcataccct tgtatagatg ctagaggtgt
     5641 ctttatatca tgtgttatat ttgccagcca ttcctctctc aactcttcat tttcttttct
     5701 ctcaacttca tttactctta aagtatcagc taacatattt atatttttaa aaacttcttc
     5761 atatatacct ctgtccttaa gatataaact ataatttcca ttagccatag tctcaatact
     5821 ccatagtacg cttgaaattg gttttgtaag ttttctacta aataaatatc ctacgcctaa
     5881 agctaccacg ctatcaatta ctagtgttat aatgataact ttatttaaag tttttttaat
     5941 gttattttga gaataggtta aaattattct tgatatatct ctactaggta tacctactat
     6001 tatatttaaa tgctttttgt ttaaatattt ttctagtaca aaattaacat actttgtttc
     6061 tatatacttg taattattca ccatttgaaa gggtgtatat ttttttggag tatcctttgg
     6121 cacatttact ccataaactt cctctcctaa atcatttaaa acctgtatcc aggaatttga
     6181 tttttctaat ttttcagctc caatcttaga tagtttaaat tctccatctt tttcatataa
     6241 atctttttca aaagaacgtg caaattcttc aggattattt ccagaatcta caactttaaa
     6301 aaagctatta gttgaaatca catacaatat agatatgata tttataatta ctactgatat
     6361 agctacaaac acaattataa atataaaatt tctagtaatc ttccacttca tattctaatc
     6421 ttcctttact gctaatttat atccaagacc ttttattgtc agaatatatt caggttttga
     6481 aggattatcc tctaattttt ccctaatttt tcttatatga accattatag tattgtcaaa
     6541 tccaacataa tcttctcccc acactttatc ccatagtttt tctttactta taatttgatt
     6601 ttgattatgt gcaaataaag ataggatttt tagttccttt gcagacagtc ctatagattt
     6661 tccatctttt ataagttctg ccctactttc atttaatata tatgggccaa atgatataat
     6721 tttttcttca ttattattta gatttttact ttcgttttga gcatcactaa aataacttaa
     6781 tcttctcaaa tgcgctttaa ctctaaaagc cacttcctta gggctaaaag gttttgtaac
     6841 ataatcatct cctccaatcg ctagtccaag taacttatct aattcttcgg ttttagctga
     6901 taaaaatatt attggagaat tggaggtttt tcttatttct ttacaaatat cataaccttc
     6961 gccatctggt aacataatat ccaatacaat caaatcagga ttaatacttt taaataattc
     7021 taatccttct tttttagttt ttgcagtata tatattatta aggccttctt tttttaggac
     7081 agtttctaaa agcttcaata tttctacttc atcatctatt attaaaacac gattttccat
     7141 aaacaacctc ctgtaataat ttacatatat attagcatat attttatcta aaattcattt
     7201 atcttatttt taattaaact aaaatataaa taaaattact attattttta gttaaaacct
     7261 tttgcttgat gtaaaatttt catatgttat tttagttaac actatcatta atataattga
     7321 gattactata gaaaaaacta tcaaaatact tggtacatta tacttaacca tttgttggct
     7381 catcataatc caatttggag tcactgacat aaattttata gctttatctg gtataaaacc
     7441 tacactaaag tatggaattg ccattatcaa tccacctagt ataaatggaa ttacagccga
     7501 tttaaatata gatgaaagtg ttaagcctat ttgtgcaaat ataaaagaag caaaaatagt
     7561 agtcaatata acaattatat acatttgtat tactgtaaga tttaaattac tatttccaaa
     7621 gtaccataag ttatgaatcg gttcatttaa atttttaaaa tcaaaaaaac taaaacctct
     7681 aattcctact aacaatataa aagaaatatt aataatagat aaaattaatc cattagctac
     7741 tagttttgat aatactactg atttttttcc ctttttagtt gtatttatta aacctgtcat
     7801 attagaatca tattctttaa tgtaatttcc agaagttatt ataaacatta tcaaacctat
     7861 tagaggtatt gagccttcta tcttgtaaaa tatatctgca taagtatcta aaacatcaaa
     7921 ctttctaatc agaaaaaatc caaaaaatat taaaaacagc caaattatcc aaacactctt
     7981 tgattttaat atttttatca tttcagcttt aactaaatac atcatttcta ctacacctcc
     8041 cttgcatctt ctaaatcaaa ataaaacatg tatacatctt cgaattttgc ttgaactggt
     8101 tgtatattat cctcgcttgg aatatcctct gaaacaattc taagtactat tgaattaata
     8161 tcactttgaa tacttacaac cttatattta gattgtatat catcaacttc acattcattt
     8221 ttaacttgaa cattatatac tttattttcc atgtgactta atatgtcttt gtgtgttcct
     8281 ttcattagga gtttgccatc tttaatcatt attgtttcct ttgcaactga ttctatatcg
     8341 gaaattatat gagtagaaag tattattatc ttatctctag acatttttgc tattaaattc
     8401 ctaaatctag ctctttcttg tgggtcaaga cctgctgttg gctcatcaag gactattatc
     8461 ttagggtcgt ttagtaaaac ttgtgctatc ccaactctcc tcttcatacc tcctgaaaat
     8521 tttcctatag acttatttct cacatcataa agacctacta aatctaataa ttctcctatc
     8581 ttactattta aatattttcc ccttaaacct tttaaagctc caatatactg taaaaattct
     8641 tttgctgtaa aatttttata aactccaaat tcttgtggca aatatcctaa aatatttcta
     8701 taatcagcat ccattttaaa tatatcctca ccattataaa gtatctgacc ttcatcaggt
     8761 tttatcaacg ttgttatttg tttcattaat gtagattttc ctgctccatt tggtcctaga
     8821 agaccataaa ctccattttc tagagttact gtaatcttgt cgtttgctac ttttttacca
     8881 tatatctttg aaagattttc tatttctaat ttattcatct taactcccct tttatctact
     8941 tatataatct gattttatta ctaaaaaaca taatgacata gcacctatca atattgccat
     9001 caaaagtcct attgctatat tgacaatgtc tatattcact ttaaatatca taatatcacc
     9061 tagataattt cctattattt tattaaaacc aaatatcaaa tcataataat ttacatttga
     9121 tacaactgaa agtaattttt taggtaatat atggattacc gacattatct tagttataga
     9181 tataataaat ataaaaaaac ttattgaaga tattgaagac ttacttataa aagacccaaa
     9241 tatggctatc atggcccatc cttcaaacat caaaagctct atgcctatat ttaatattac
     9301 atactttgtt atacttaaat tagttttcaa tgcaactaca ttgtctacta ttctaaaaga
     9361 ttctaagtct ccattaacag gtactccttg ttgcatacaa gttataataa atattcccat
     9421 taaataaaca gaataaatta ctaatggaac taataaagca acaaatattt tagactttaa
     9481 tgcttttttc ttctcttttg atgatagtat gatatctttt accgaagtaa cgacttcatc
     9541 tacgtaaata ttagatataa ttatagatat aatgattaac attcctacat ttataagtga
     9601 atttgtaact ctatagttaa atactttcca aaaactaata tccttatatt cattcacact
     9661 tagattatca agttttttct ttgacattaa ttcaattttt ttagaaaatt cacctgtatc
     9721 tgtttgattt gaaaactgct ttaaaacatt aatcttattt tgaaatttct cattacctac
     9781 gtcgatacca cttctcgtat cttctatacg tcctttttct ttatctatat aataactttc
     9841 tgtttctaaa acaggcgtaa taaagatact aatcattagt attaacacta atatccctag
     9901 agatataaac atactcttag atttagccaa tttttttatt tcccaattta ccaatatatt
     9961 tctcctccta aatcatttta attacacata tatattaaaa cttaa
//
LOCUS       DACXMZ010000147         9988 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.148, whole genome shotgun sequence.
ACCESSION   DACXMZ010000147 DACXMZ010000000
VERSION     DACXMZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.148"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            87..1517
                     /locus_tag="JD016_13590"
     CDS             87..1517
                     /locus_tag="JD016_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAU5000665.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1620..2723
                     /locus_tag="JD016_13595"
     CDS             1620..2723
                     /locus_tag="JD016_13595"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAU5000666.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            2738..2974
                     /locus_tag="JD016_13600"
     CDS             2738..2974
                     /locus_tag="JD016_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAU5000667.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            2991..4040
                     /gene="vorB"
                     /locus_tag="JD016_13605"
     CDS             2991..4040
                     /gene="vorB"
                     /locus_tag="JD016_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HAU5000668.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4042..4782
                     /locus_tag="JD016_13610"
     CDS             4042..4782
                     /locus_tag="JD016_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HAU5000669.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            4785..5324
                     /locus_tag="JD016_13615"
     CDS             4785..5324
                     /locus_tag="JD016_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAU5000670.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5448..7547
                     /locus_tag="JD016_13620"
     CDS             5448..7547
                     /locus_tag="JD016_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HAU5000671.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(7814..8329)
                     /locus_tag="JD016_13625"
     CDS             complement(7814..8329)
                     /locus_tag="JD016_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HAU5000672.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8421..9635)
                     /locus_tag="JD016_13630"
     CDS             complement(8421..9635)
                     /locus_tag="JD016_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HAU5000673.1"
                     /translation="MMSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTK
                     MTLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVI
                     ISDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDI
                     NGTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYL
                     HIMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIK
                     RIESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLI
                     DCSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIV
                     SYNFFNGSINFY"
BASE COUNT         3752 a         1227 c         1846 g         3163 t
ORIGIN      
        1 caagaatgag atatttaaga ttcaaaatat atattattat acgtgaataa taaaaagtaa
       61 gaaaatttta taagggaggg gtagatgtga gtagtcaaga aagcaaacca aaaggaaatc
      121 ttatatcaaa ttttagagat gatatgagag accctaagaa aagagcaata tacacatatt
      181 caagttctat aaacttagcg tacaatttta taggttactt tgtatcaact gttacaaata
      241 tttttttaac tgatgtggca aaacttggtg taattatggc aggtggtatg caaattatac
      301 aatctttcat aaaaattgct gtagctccta taacaggtac agtatttgac aaacaaccat
      361 tcaaaaaagg aaagtattat ccatggctta gatatgcacc agcagcgtta gcattaacat
      421 atggaatatt tttcatagtt ccattaatga aaataagtcc agcattactt ttacctttga
      481 tgacagcttt attattaata gcatcttgtg ctcagcaagt agttttcact ataatttatg
      541 ctatttaccc tgtagttgct aagacaccta gagaccgagt tataggctca actactacga
      601 atatatttaa agaagttggt aaattcttag taggtttttc atatccgtta ttattagttt
      661 tatttgcaga tgtattaggt ggagaaggaa tgggttattt aggtacctac ttattctttg
      721 cagcaggatg ccttttagta ttttggtttt cttcaacaga gataataaaa tctggagcag
      781 aagaagaagt tcttcaaaaa gaagagaagc caaagattaa agcaagtgaa atggcaaaag
      841 ctctattcac taatgtacca cttatgatgg cattttgttt agaatttctt atatgtataa
      901 gatctatagg attaggacct ttggctccat attattttaa atatgtaata gaagatgaac
      961 gtggattagc agtattttta tcaatcatgc ctttggtaag tgtagcattc atgtttttcg
     1021 caccagtgtt tataaaaatc tgtagagagc aaaaactagc ttcaataatt tctttttcta
     1081 tatgtgcact atgccactta ttggttgcat ggactccttg ggggaaaact actttaggag
     1141 taactatgtt gttggcaatt ggtggaggtt tttcaaatgt agtatcaata ataaatctta
     1201 atttctttgc cggttcttgt gattatgggc attggaaatc aggaaaagac ttacctggct
     1261 taagtatgtc actatatcca gttgcaatac aagtaggagt attacttgca actactataa
     1321 gaacagtttt aatgaactca atgggatatc aagctgatat ggtagtcaca gaagcagtta
     1381 aatcaggttt tataaatatg atttcttatt ctatggcaat acctttaata atagctgttg
     1441 ttatagcaat actatatcca gtaagtgaca agaaattaaa ccaaataaga gaagagttaa
     1501 atcaaagaaa tgcttagtta aaagaaagtt aagtagatgg ttaatttaag ctattaaagt
     1561 aagtagacgc aatagcgttt atataaatga aaatacatat acagaggggg cttttaaaaa
     1621 tgatagaaaa attattgaga aataccgtaa aagaattaca tcaatatgtt ccaggtgaac
     1681 caatagaaaa agttaaggaa aaatatggtg taaaagaaat aataaaatta gcttcaaatg
     1741 aaaatcctct aggaccatca ccaaaagcaa tagaagctat gattgaaatg ttaaaacaag
     1801 gtcaacttta tccagagcca gaagctaacg aacttagaag aaaattagct gaaaagttag
     1861 atttaaaacc agaaaatttt attgttgcta atggtgcaga taacgttatc actctaatag
     1921 gtgaggcttt tataaataga ggagatgaag ttatatattg taacccaaca tttccatcat
     1981 atagaactgc aactataaaa aatgaaggga taccagtaga agtaccttta acagaagatt
     2041 ataagtatga tttacaaggt atattagata agattacaga taaaacaaaa ttaatatgtg
     2101 tatgtaaccc taataatcca acaggaacaa ttgtggatga taaagagtta gaagaatttt
     2161 taaagaaagt ccctgaaaat attataacta tattggatga agcatatata gaatttttaa
     2221 cagttcctaa ttatgtggat ggacttaagt atgtaaggga aaattataac gttatagtaa
     2281 caagaacttt ctctaaaata tatggtcttg cagggcttag agtaggatat ggtattgcta
     2341 aagatgagat aataagaact ttatttacag taaaagagcc tttctctgca aatcgtgttg
     2401 ctataagtgg agcaacagca gcacttgatg atgaagagtt tataaaagaa acatatgaat
     2461 taaatagagt tggaatggct tactttaaag aagagtttac aaaaatggga tttgatgttg
     2521 tagattcaca atcaaatttc ctttatgtag atatgaaaac agatatacct aagttatttg
     2581 aagacttgaa aaaaagagga tttgtaataa gaccaagtgc tacacatgca cgtgtaagta
     2641 ttggaactat ggaagaaaat aggaaatttg tagcagtttt aaaggaaata ttaaatatta
     2701 aaggagagcc aagtattgga taatagatag aaatattatg aaacggggtg tagatatgaa
     2761 cttaaaaata aacgcagaga gatgtaaaag ttgtgaatac tgcgttatat cttgtaaaaa
     2821 aggagcatta aaaatatcaa gtaagataaa taaagaaggt tatgctcatg tagaagttga
     2881 tgagagtaaa tgcatacttt gtggaatttg ctatcaggtg tgtccagata atgtttttga
     2941 aataattaaa gaagaagcat gtcaagaggc ttagaaggag ttgggataat atgaaaaaga
     3001 tgtggaaggg gaaccacgcc attgcagaag cagccctaag aggaggttgt gaattttatg
     3061 caggatatcc aattacacca caaacagagg taatggaatt tttatcacat agaatgtctg
     3121 aacttggaag aacttttata cagtccgaaa atgagatggc tgctatatat atggtatatg
     3181 gagcatatgc aagtggaatg aggtctatga cgagttcttc aggaccagga atatctttaa
     3241 aacaagaagg gatttcttac ttatgtgcaa atcattaccc ttgtgtaatc gtaaatgtac
     3301 agagatgggg gcctggtctt ggctcattgg actcagcaca gacagactat ctaagagata
     3361 ctagaggggg aggaaatgga gattatcatt tgatagttta tgcaccaaac agtattcaag
     3421 agactgtaga tttgatgtat aactcatttg atgtagcgga gaagtacaga gtgcctgttg
     3481 aaattttgac agaagcagct ttaggtcaaa tgatggaacc agtagaattt ccagagttta
     3541 agaagagaga aggagattta ggttggactt atgatggaag taatagagac catgcaaagg
     3601 ttgcagataa ccaaaagcca actttttgta tggagaaacg tatgagaata actgaaaatg
     3661 aacaacaatg ggaagattat caagtagaag atgcagaata tgtgtttgta gcgttcggta
     3721 taccatcaag aacaactatg aatgctgtta gaagacttag agaacaaggt gaaaaagttg
     3781 gaataataag accaataaca gtatggcctt tcccatataa agcatttgaa aaggtaagta
     3841 aagatgtaaa aggatttatc tcagtagaat caacagacac aggtcaatta gtagaagatg
     3901 ttgctttagc ctcaaagaaa gtatgtaaag aaaatgtacc tgtgtatggt ttatttagtg
     3961 gaaatcatat accaaaaaca ttgcaagtaa tggacactta tagcaaaata aaatctggcg
     4021 aaatcaagga ggtgttctaa tatggctaca gaaaaattaa cacctagcgt tgtttcaata
     4081 ccaaacaagt tttgcccagg ttgtggtcat ggaatagtaa atcgtataat agcagaggta
     4141 atagaagaac atggatatga aaaaaatcat gtattaacat taggagttgg ttgtgtctgc
     4201 aatatgaatt ttagctggaa tggtgataag atgcaaactg cacatggtag agcttcatct
     4261 acagctatag gagttaaagt agcacttcca gatacattag taatgactta ccaaggtgat
     4321 ggagatgctt atgttatagg tctttctgaa actttaaata cagcatatag aaatcataat
     4381 gtgactgtat ttgtaataaa caataataat tttgcaatga ctggtggaca aatgtcatgg
     4441 accactatgc cgggtcaagt cacaacaaca agtgtaaatg gaagagatat aaagactact
     4501 ggtagtccac tgaaagttcc tgagatggtg gcaaacttct ttgatgtagc gtatgtagca
     4561 agaggttcag ttcatagccc aaaggaaata attaatctaa aaaaatatat aaaaaatgca
     4621 atagaagctc aattaaatgg agagggatat tccttagttg aaatattagc accttgccca
     4681 actaactggg gtgtatcact agagaaatct atcaaatgga tggaagaaga aatagtgcca
     4741 tattatgctc ttggcgaatt taagcaaagg gatggtgaat agttatggaa aaagagctag
     4801 tatttgcagg gtttggtggt caaggagttt taactcttgg tctgattgta gctgagtcag
     4861 cacttgaatt ggggaaacaa gtaacttgga tgcctgcata tggtccaact atgagaggtg
     4921 gaaaagccta tagtgtagta aagttttctg atgagagtat aggtgggcct gatatggaag
     4981 aaatagatgt tttagtagca atgaataaac catctttaga ttatataaat ttagttaaag
     5041 aaggtggaac agtagttatt aatacatctg caatagatga gaatgtaaca ttaagagaag
     5101 atataagtgt agttaaaata aattgtcagg aattagctca aaaagtaaac aatccaaaag
     5161 cagcaaatat agtagtactt ggagcattaa ttgctaaaac tgggttattt gaaaaagaat
     5221 tagctttaaa aactatgtgt gatttctttg aggaaaaagg aaaaggaaag tttaatgttc
     5281 aaaatgaagc agcattcatg gaaggctata ataacgccat ttgataaatt acccttatct
     5341 taagtgattt atctcaagat ttaataatct ttagtgccaa gagaaacttc atcttgagat
     5401 aaaattcact taaaatcaca actataaaca tataggagcg tgaatttatg agtttggaaa
     5461 aacttattag accaaaaagt attgctatag taggagtgac tgataaactt ggttttggaa
     5521 gaagtgcagc acttagtata gtaaaaagta aggaaacaga tagagtatat tatgttaatc
     5581 ccaaaagaga agaattgttt gggagaaaat gttataagac gatacaagaa gttccagaag
     5641 tagtagactg tgtagtagta tgtacgccta gaaatgtagt tccatctgta ttaaaagatt
     5701 ctggagagtt aggaacaaag gcagcagtgg tatatgctag tggatttgca gaggaaggta
     5761 cagaggaagg tacagattta gaaaatcaac ttatagaaat atcaaataca tacgatatga
     5821 aaatattggg tccaaactgc atggggttgc taaattgtat agataaggtt aatctatggg
     5881 caggaggttc taaatgggat ttagatacta aaaaacctgg tataggaatt gtaggtcaaa
     5941 gtggatttat aactgctgaa attgtttcaa gtgattattt taatatatct tatggttttt
     6001 caacaggaaa tggaaatata gttacactag aagatgcagt agatttcctt gtagatgatg
     6061 attatgcatc agtaatagca atttaccttg aaggtttaaa aaatcctcaa aaatttatag
     6121 atgctttaaa aagggcagca caaaaaagaa agccagtaat aatattgaaa tcaggaaggt
     6181 cagaaaaagg tgctatttct gctgcttctc atacaggaaa tttagctggt tcgagtaaag
     6241 cttttgaaag tatttttgaa aaatatggag taatttctgt ggaaaattta gaacagttta
     6301 tgtgtttagc acaagctttt agtgttttag atggtaattt acctgcaaat tctaattttg
     6361 cagcaataaa cttctcagga ggagaaaata ctatttgtgc agatttagca gaagaaaatg
     6421 gtgtagaact tgcagaaata tcttctgaaa ctaaagaaga aatgaaaaaa tatcttccag
     6481 gatttgcaac acctaaaaat ccactagatg ctacaacagc attattccat gaaaaggata
     6541 tgatagtagg tttattacat acctttgaaa aggatgatag tgttggattt actatggtag
     6601 gtgcaaatat aagagatgaa gaaaatgaaa tgcatgaaac cctttgtaaa gctgtaagtg
     6661 aagctagaga acaaggattg aaaaaacctg tgtttgcagt tcctacactt gaaggaaata
     6721 gatatttaga ttataggact agattagaag ataatcaagt gcctataatg tcatcagttg
     6781 gaactacatt tacttgtttt aataaaatgg ctaagtttat agattatgat tattcaaaaa
     6841 gaactttaga atttaaagct gttaagaaaa gagagtcaaa taatgtagta gcattatcag
     6901 aattagatag taagattgag atgaaaaaat atggagtacc agttccagga caaggtaatg
     6961 caaaaagtat tgatgaatta gatgaaatct taaaatatat taagtatcct gttgttttaa
     7021 aaataaattc ttctgaaata ttgcacaaat ctgatgttgg aggagttaaa ataggaatta
     7081 agaatagaga tgaggcagta gatgcatata atgagatttt aactaatgta aaaaaagcaa
     7141 aaccagatgc taatattgat ggtatattag ttcaagaaat ggtagaaagt ggtatagaaa
     7201 ttattattgg tataactaat gatgaccaat ttggaccaat gttattagtc ggtttaggag
     7261 gagtatttgt agaggtattt aaagatacta cattatatcc attacctata aatcatgatg
     7321 aagctattat gatgcttaaa aaactaaaat catttaagct tttaaatggc tatagaggaa
     7381 gtgagccttg cgatattgat gctcttgcag atatgatggt caaattagga aaatatgctt
     7441 atgaaaataa ggatgaagtc aaggaaatag acttaaatcc agtgtttgta tatcctaaag
     7501 gaaaaggcgt atgtgcagta gatgccttaa tagttaaata taaatagttt ataagttttg
     7561 gtattcaacc ataattaaag caccgactga aaatcacatt ttgtaagtta gatagtgtga
     7621 ttttcagtca ccccaaattt aatttaaaaa tattttaaaa tgaaagtata gttttaagtt
     7681 tcattgtgaa atagcactgt ataaacaata aaatactacg tataaaacga ataataaaag
     7741 gtgttttaat gataagaatc aatcttgatt ttcattaaaa ccccttcttt attatattaa
     7801 aaatttttaa acgttatatt gtcacaggtg ggtttgttgg agctgcatat gtacgattag
     7861 caaattcttg gtcaacaaag aatagaccac cctctccagc aagtttaaca cgttttatat
     7921 tgtcttcaca atttgtctct tcttctgctt gttcagatat aaaccaaagt aagaattgag
     7981 aagttctaaa gtctgattca gagtttgcta cagctgctaa gttgtttatt aaagctgtaa
     8041 cctttttttc atgagctaac gtacgctcaa gaatatccat tgcagaagta aagtctgaat
     8101 ctggcattgg gatagcatca agctgaacct ttcctcctac attatgtaag tatttgtaaa
     8161 aaaacatagc gtggtctgtc tcttctttat attgaacata aaaccaatta gaaaatcctt
     8221 taagtccttc tgcttcaagg taagaagaca tagaaagata aagatatgca gaatataatt
     8281 catggttaat ctgctcatta agtaattttt ccattttagc tgaaatcatt tttatacctc
     8341 caaattttaa atgctcttat aaaatataat aatattaaat ggaaaaatat tcaataaaag
     8401 tatttaaata ttttacatat ttaataaaaa tttatactac cattaaaaaa attataactt
     8461 acaatggcta tagagccaat taatggtgca tttgctccaa aatttgaatg ccttacactt
     8521 atttttttat atttagaaaa acttgctgaa gcagtcaatt tttttaataa tgtattttct
     8581 ataaatgtac caggtatatt aaagtcatag cctattatta tagaactaca atcaatcaat
     8641 gtaagtgtat taactaatgc atatgatacg tatgtacaaa attcatcaag caaaaacatg
     8701 gcaagactat ctttattatt tgcctcatct attaacttga ctaaagaaaa atctttacag
     8761 ttaattaatg gggagttagg ataaatatgg ctcaatgatt caattctttt aattagatta
     8821 ctgacacttg tataataatc taaacatcca ttgttaccac aactgcacaa tggtccacta
     8881 aaattaattg atgtatgtcc tatttctcca ctttgtccta ggttgccagt atgaagttta
     8941 ttttctagaa ctagaccagc tccaatacca ttcattatgt gtaggtatat ataatttgat
     9001 atatttttac ctaaaccata cattttttct gctaatgcac ccgaattagc atcatttatc
     9061 aaaaatattg gcaaatcaga catttctttt ataaaatcta ctatatttag gtttgataca
     9121 tttccaaaat ctgggggatt tagtatggta ccatttatat catctactgg acctattgaa
     9181 gaaataccaa ttccaattat atctctcttg ttgattttca ttattttagt aaacattctt
     9241 tttattatat cttttaaaac atcatttgat agacatttag ggtaagtttc agaaagttgt
     9301 tttacaatat taccttttaa atctgaaata attaccttac atatatttct tcttattagg
     9361 attccacaaa tacaaggcga tatattagaa atatctaata aaataggttt acgacctgat
     9421 gaatgtgcat ttaaagttga tgactctatt tccctaatca tattttcatt aataagttca
     9481 gtaactagat ttgataaagt cattttagta agtcctgtag acttagctaa atcaactctg
     9541 gacataggtg tatttgtaga aagtaatttc aatattatta acttattttt aatttttaca
     9601 tctttgatgt tagaaccttg tttgttactc atcataaatc tccttaaata aagttaagtg
     9661 ttttatttat tttcaagtat actatcacct tataatagag taacattttt ttacttgaaa
     9721 atatcattaa ttagaatata tcacattata tatcataaaa tatataaaaa aatgttagaa
     9781 ttaaattaaa agtgaaaagt agattgatat ataaatagta aatacttaat taaaaaaaga
     9841 taatgatagc aataattatt gttaccaaga atctactact tcaagtgtta ccttaggtat
     9901 ttaagttgat agtggtttgc acgtaataaa cttgatgtaa tatatattct taataatatc
     9961 gataaaagat ttacttaaaa ataatata
//
LOCUS       DACXMZ010000148         9974 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.149, whole genome shotgun sequence.
ACCESSION   DACXMZ010000148 DACXMZ010000000
VERSION     DACXMZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9974
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.149"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            209..1453
                     /locus_tag="JD016_13635"
     CDS             209..1453
                     /locus_tag="JD016_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAU5000674.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            1776..2873
                     /locus_tag="JD016_13640"
     CDS             1776..2873
                     /locus_tag="JD016_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAU5000675.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            2864..3562
                     /locus_tag="JD016_13645"
     CDS             2864..3562
                     /locus_tag="JD016_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAU5000676.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKKVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            3565..4629
                     /locus_tag="JD016_13650"
     CDS             3565..4629
                     /locus_tag="JD016_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAU5000677.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            4812..5516
                     /locus_tag="JD016_13655"
     CDS             4812..5516
                     /locus_tag="JD016_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000678.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            5509..6831
                     /locus_tag="JD016_13660"
     CDS             5509..6831
                     /locus_tag="JD016_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU5000679.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            7018..8391
                     /locus_tag="JD016_13665"
     CDS             7018..8391
                     /locus_tag="JD016_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAU5000680.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(8763..9701)
                     /gene="pdaA"
                     /locus_tag="JD016_13670"
     CDS             complement(8763..9701)
                     /gene="pdaA"
                     /locus_tag="JD016_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HAU5000681.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         3715 a         1105 c         1682 g         3472 t
ORIGIN      
        1 ttatatccaa ttaaaagaat tagacaatat tatttattat attttaactt aaaaataaag
       61 aaagtaacta tgttaaatat ggaaaaatgg caaaacactt gactattaat tggtaaagga
      121 ctataataat aaattatcat attgataaaa acgaaaatta tgaaatgtct gtaaaaataa
      181 aatttaaata ataactagga ggagtaatat gtttgagaac ttgaaaaaag cggaccctga
      241 gatttatgaa agtatgaaaa gagagttaaa aagacaacaa agaaatattg aattaatagc
      301 atctgaaaac atagtatcag ttccagtaat ggaaactatg ggaagtcatc ttacaaataa
      361 gtatgcagaa ggttactcag gcaaaagata ttatggtgga tgtgaataca tagatgaagt
      421 tgaaactctt gcaatagata gaatcaaaaa aatatttgga gcagaacatg ctaatgttca
      481 acctcactct ggagcaaatg caaatattgg tgtatatttt gctatgttaa aaccaggtga
      541 tgttgttatg ggaatgaatt tatctcaagg tggacattta actcatggtg ctccagttaa
      601 tatttctggt caatattata aattttatga atatggtata gataaagaca gtggacttat
      661 agattttgat gaagttagaa agatagcaca tgaagtaaaa cctaaaatga tagttgcagg
      721 agcaagtgca tatcctagag aaatagattt taaaaagttt agagagatag ctgatgaggt
      781 tggagcattg cttatggtag atatggctca tatagcaggt cttgtggctg ctggtcttca
      841 ccaaaatcca tgtgaagtgg ctgattttgt tacaacaaca actcacaaaa ctttaagagg
      901 acctcgtggg ggtgttattc tttgtaaaga aaaatatgct aaagacatag acaaagcaat
      961 attccctggt attcaaggtg gacctcttga acatataatt gcttctaagg cagtttgctt
     1021 taaagaagct ttaagtgatg agtttaaaga atatcaggta caagttgcta aaaatgcaaa
     1081 agccttagct gaagaactta ttaaaagaga ttttaaattg atttctggag gtacagataa
     1141 tcatttaata ctattagatt tgactaataa aaatataact ggtaaagcag cagaaaagag
     1201 attggatgat gcttatataa cagctaataa aaatacaatt ccatttgacc caaatggtgc
     1261 tcttgttaca agtggaataa gacttggtac tccagctgta actactagag gtatgaaaga
     1321 agaagatatg gcaataatcg ctgaggctat agacctatgt ttaacttatg atgaagaatc
     1381 taaggctaga acattggttg ttggtttaac tgaaaaatat cctttatatg aagaatataa
     1441 tattatggat taattataaa gaacaactaa ataaaataca tttaaataat tacaatgatt
     1501 gtgtatgtat ttgaatatat ttagatataa aaaaggtgtc tcaaaatgaa ctttttagtt
     1561 tatgaggcac ttttttgtat ggaatcgaac atatttgtat tatttaaata ataaaaaata
     1621 tggttatctc tatttttaga tggtctcttt gtatttttta attaaaactt taagatttct
     1681 taagaattat actaggtttt tctaaagatt taaagtctat acttataagt atagtaatca
     1741 tatgaaaaga aaagctttaa cgaggaggga atcttatgaa ggtattaatt cttacaggaa
     1801 aatttggtat gggtcattac tcagcatcaa attctttaag tgaagatata aaagcaaaat
     1861 ttgataattc agaaataata attaaagata tatttgagta cattatgcct aactattcag
     1921 ataaaatgta caagactttt tctattcttg taaatcgggg aagtagtctt tataatttgt
     1981 tttataaatg tgctgaaaat ggaaagaaag atataaaatt tactttttca gattattttt
     2041 taaataaatt agatacatta cttcatgaag tacaaccaac agtagttatt tcaacttttc
     2101 cattttgttc tcaattagta tcaagatata aggaaaaata taactcaaat cttcctttaa
     2161 taacatgcat aactgatata agtagtcact cagaatggat tagtaaaaat acagactgtt
     2221 atttagtagc tagtaaatct actaaggaag aacttgtttt taaaggaata gatgaatcta
     2281 agattaaagt taatggtatt ccagtaaaaa aagaatttaa aagaatagaa catgtaaatc
     2341 attcaactaa aaaaaatatt cttataatgg gtggaggatt aggattactg cctaaatcag
     2401 aacaatttta caaagaatta aatagtttag aaggagtaaa aactactgta ataactggta
     2461 ataataaaaa aatgtactat aaactatatg gtagatatga aaatatagag gtagtaggtt
     2521 acaccaatga agtatataaa tatatgaaag attctgacct tattatttct aaaccaggtg
     2581 gaataacact atttgaaaca atttattcag aacttccaat attggcattt aatccatttt
     2641 tacaacaaga gatagataat gctagtttta tattaaataa tgaaattggg agaatacttg
     2701 gtaaaaataa aaagtactat gtagatgaga taaaagattt aatttatgat gatgctactc
     2761 ttaaagaaat gagcagtaac atgaaagaat taaaaaaaca atttgataat aataccttag
     2821 agaatatttt attttcactt gatgaacaag gagcgtgtag agaatgtatg tagtaggttt
     2881 aattatcgtt gttgcattga tttttttagt ccattcaatt atacctacat actataataa
     2941 actattaaat aaaaaggtat taaaaaatat ggctggagaa aatgaaattg cacttacatt
     3001 tgatgatggt ccagataaga gatatacaga aaaattacta gatgtgttaa aagaaaatga
     3061 tatacaagct atgttttttg tagtagcaaa gaatgctgaa aaagagcctg aaattataaa
     3121 aagaatgttg agagaaaatc atatagttgg tttacattca ttggaacaca gaaatgcatg
     3181 gctttatagt tatagttatg ttaaaaaaga ttttattgaa agtacaaata taatgaaaaa
     3241 tttaggtgta gatgtaaact actatcgtcc accatggggt catactaata tattttcaaa
     3301 ttcatttgtt aaaaaatata atctaaaaat gactttgtgg gatgtaatgg cagaagattg
     3361 ggagaaagat agtacagtag acattattat aaacaagtta atgagtagaa caaaagaaaa
     3421 ttctataatt tgtttacatg atgcaggtga aaattcaggt ggtgcagtag gtgcacctga
     3481 gcgtactatt gaagctttaa agatagctat tccaaaactt aaagccagtg gtttaaagtt
     3541 cgtaacacca gaaaggatgt agttatgata agtaaaactg aaaagaaaaa taaatgccta
     3601 ggtagtgtag cttttttagt tttactaatg ggcataacag gatattttgt ttttagaggg
     3661 cagtcagttg aatcattgat aaaatcatta aaaggtgcaa gccctatgtt tatactaatt
     3721 ggatttgcaa tgatgtttat atatgttgcc tgtgaaggta taaatatata cttaggtatg
     3781 aaagctttaa accaaaagac tactctttta aaatgtatgg gatatgcttt tattggattt
     3841 tattttagtt ctattacacc ttcagcatca ggtggtcaac cagcacaagt ttattatatg
     3901 aaaaaagacg atataaatat atcatattct tcacttatac tattagtcat tgttgttatt
     3961 caccaagtag ttatactggc atatagtgga ataatgttca ttatggaacg tgagtttata
     4021 ttaaataatg taagtggtat gaacatttta ctaatatatg gtgtaataac aaatgttgct
     4081 cttgtcattg gagtaattgc aattatattt tcaaaaaaac ttgtaaacaa ttttataata
     4141 tcaataacaa atttattagg taagttaaga ataataaaag atgttgaaag ttcaagaaaa
     4201 gttataatat ctcaaattga agagtatgtt aaaggtgctc aatatataaa gcagaatcca
     4261 aaacttgtag tccaaatttt agtcataact attgtacaaa taacagctat gtttttagtt
     4321 cctttctttg tatacaaagc ttttcatcta tcaacatata ctgtatttga gatattagcg
     4381 atacaatcct tgcttaatat tgctgtttct tcattaccat tgccaggggc tgttggagct
     4441 tctgaaaata gttttatgac attatttaag atatttttcc ctggtcactt attagttccg
     4501 gcaatgttac ttagtagggg gataagtttt tatgcatttg tagctataag tggattgata
     4561 tgtatagtag tacatgtaaa atcttctgaa aaagtaaaag cagtgaaaaa agttgcttat
     4621 gtaagataat attatataga ttatttttat taatatgtca atttgtgtgt atattttaat
     4681 taagctttaa acatgtttac aggagtagaa actattaaaa cgtgtgacat tgctaagctt
     4741 ttgctaataa agtataatat tttatatagt ttgaaatatt taagaaatat agcaaaaaaa
     4801 ggagcttggt gatgaataaa gagattccaa aaaaaatttt agtagctgat gataatcctg
     4861 aaattaggga aatcgtagaa atacttttaa ctggtgaagg ttatgaagtt attatggcaa
     4921 cgaatggaga agaagctgta aacttagtag atagtacgat tgatttgatt attttggatg
     4981 ttgtaatgcc tgtaaaaaca ggatttgttg catgtaatga aattagagaa aaaacgacag
     5041 caccaatatt atttttaact gcaaaaactc aagactctga taaagttatt ggattttcag
     5101 caggaggaga tgactattta tcaaagcctt tttcatattc tgaactaatt tcaagggtta
     5161 agtctctact tagacgttat tatatatacc agggaaagga aaaggaaaga gagccaaata
     5221 tattaaaaac aaaagagtta tgtgtcaatt tagatactca agatgttaca ctaaatggag
     5281 aacctgtgtt tttgacaact atagaatata gtatattaat tcttatgttg aaaaatagaa
     5341 aaaaagtttt ttcgtctgaa aatttatatg aaactatttg ggatgagcag tatttttata
     5401 cagcaaataa tacaataatg gttcatatta gaaatttaag aaaaaagtta gaaaaagacc
     5461 aaaaaaatcc acaatatata aaaaccgcat ggggaaaggg gtattatatt gattaacaaa
     5521 attaagccat ctaagttggg agttatacta ttgatatatg tagtaatctc agctgcagtg
     5581 gctttcattt taaatatacc attttactct gctgttgaat ctatagtaaa agatgtatca
     5641 gaagggaaag tatattttaa aagagaagta aatcaactta tggataattt tcaaacttat
     5701 gtgaataaca atggtataag agcagatgat ggagatgata tagcaaaatg gaatagtgat
     5761 aattggtttg tttctatgaa gatatataaa ggaaataagg ttttttatga tacattatac
     5821 tatcctaatg aagtacctga taagagtgag gaaaatgagt tttatagcag tcccatggct
     5881 agaaactatg agatacaatt ttctgatatt aaagctaata tagttgtagt agctttattt
     5941 agatttagat ttcaaaattt tatagaagca atttctatta catttatggc acttacattt
     6001 attataacat ttttactgtt gtttagtaga aagataaagt acctaaggaa aatagaaatg
     6061 ggtataaaga tagtagagag tggcagttta aagtatagaa taccagtaaa aggtaatgat
     6121 gaattatcat cacttgcatc tagtattaat gagatgagtc atacattgga gaatcaactt
     6181 ataaaagaaa gggagatgaa gaataaaaat aaagatatag taacttcaat atcacatgat
     6241 gttagaactc cattaacgtc agtaatttgt tatttagatt tgattaaaga ccataaatac
     6301 aatagtcctg agcaattgga acaatacata aaaaatgtaa ggtctaaagc atatcaaata
     6361 aaagatttaa cagatgacct ttttacgcat tttgttaatt ctggagagga agtttcaaaa
     6421 gggtatgaaa aaataaatgc taatgaattt atttttcaat tacttttaga tagttcatat
     6481 acattggagg agaaaggatt tcatataaca atagaaaatg aactttataa ccaatatcat
     6541 atttatgtag atgttacaca atttagaagg atatttgata atttatgttc taatataata
     6601 aaatatgcta ggaaagataa gcctatagta tttttaatta aagtagagga ttctaaatta
     6661 ctaattatac aaaaaaatag agtaagtaat ttttctaaga atgtagaaag ttttggaatt
     6721 ggtataaaaa attgtgagaa aatagttgca tatcataatg gaagcataaa tgtaaattca
     6781 gatgataaag aatttgctat tgaaatatct attccagcaa aagaatcata aaaatctaaa
     6841 ttatatattg tgtaattgct attatatatt gtgtaattgc ttatgtaaaa ttctatgaag
     6901 caggaagcat gtgactttag ttatgggatg ttcaatattg tgtacctcta ggcaagtaac
     6961 aagtttactt ataagaccat ataatattat tatcttaaaa aaaggggaca taataatatg
     7021 gttttgacag gagcacatta tatttacatt ctatttatgg taattatatt aattactatg
     7081 attatgaaaa aggatacaat agtaccatgt atattaggtg ttttttttat ggggttgttc
     7141 tttgaaaaaa acatttttgg tgctataaca gcagtgttta actcttttat tatatcttta
     7201 aatgagttgg gtccaattat cttgattata gctattatgg ttgcattatc taaagcccta
     7261 gaagccaata atgctataca gtatatggtg agaccatttt cgagagttat aaaaaattct
     7321 aatacagcat tttttgtaac tggatttgtt atgttagttc tttcttggtt tttctggcca
     7381 actccagctg ttgccttagt tggagctgta tttttacctg ttgctatgag agctgggcta
     7441 ccagcaattg gtgttgcagt agcattaaat ttatttggtc atggtcttgc cttatctaca
     7501 gactttgtta ttcaaggagc tccaagtata acagctggtg cagcaggtgt tgcagtatca
     7561 tatgttataa atgatggtat gattttgttt tgggtgatgg gaatcgtaac catatctatg
     7621 gcattttata cattaaaaag agatattaac aaaggaatgt ttagagaaga actaaaaggt
     7681 tttgaaagtg aagaagtaga agagttcaat ggaaaatcta aaatagcaac tattttagta
     7741 gctttgggat ttcttgctga tataattgct atgtatgtat ttgacctaaa aggaggagat
     7801 gcatcagctc ttttgggtgg aacagcagta tttttaataa taattataaa tgttataaat
     7861 tttggcaaag atagtcttga aaatgtatgt gagaatataa ttgatggttt tgtgtttggg
     7921 ataaaaatat ttggagcaat tataccaata gcagcatttt tctatatggg tgaagttgca
     7981 ccattgacag gtgtatttgg aaaagtatta gcaccaggtt cacaaggttt attatcagat
     8041 ataggcatag ctctttctca aacaatacca cttaataaat ttgccgtatc aggtatagaa
     8101 actgtagtgg gagcaataac tggacttgat ggttctggat tttctgggat ttcattagtt
     8161 ggttcacttg cctctgtatt tggaactgct ataaatgcaa gtgttggtgc actagctgca
     8221 ttaggacaaa tcagtggtat atgggtaggt ggaggctgtc tagttccttg gggtcttatt
     8281 tcagcagcgg caatttgtgg agttagtcca atagaattgg caaagcgaaa ctttgtccca
     8341 gttataaccg gattggttgt aacaaccata gtagcaatgt ttattattta ataaatcgaa
     8401 taaatcttta attgtataaa ttaaaaatag atgtgtagct ataatttatt ataattgttt
     8461 tactaattct aagccataca tctattttta ttgatatttt tattagtatt ctttaataaa
     8521 ataatatttt tcattatact atacataaca tctaaatata acatctaaaa tactgttatc
     8581 tatatttaat atcttgccaa attttttgat gttttattgc cctgtcccac caacatatat
     8641 aagctatcac aagtttttac ctatactatc accaaacaaa aattctaatt ttaatttatt
     8701 attttataac ctatattata ttaactatat tagtttgtag actttagatg taattataaa
     8761 tattaatact ccaagagttc aaattcatat cctagttttc ttgcatttga tataaaatca
     8821 tttaaaattt ctgtactagt tttggaaact gcatgtagta acaagataga accatcatgt
     8881 agattatcca ttattttttg cgttgcttct tcatgtgatg gttgtttatc agtatcccag
     8941 tctctgtatg caaaactcca aaatacagtc ttgtatccta aatttttagt cattgccaag
     9001 cttttttctg agtattttcc cataggaggt ctaaaaaatt taaccatatc tttcccagtt
     9061 acatctttat ataacttttc aacatcatat aattcgtcat taaagttttt tagattgctt
     9121 gtttttgttg gcatagatgg atgggtttta ctatgatttc caactatatg tccctcagaa
     9181 accattcttt ttactaaatc tttattttct tttatatatg gagctgtaac aaaaaaaact
     9241 gctttaacct ggttttgttt tagagtgtcg agtatctttg tagtgtatcc attttcataa
     9301 ccttcatcaa atgtaagata tagtgttttt tgaccatctt ttgtaggtcc attgtatagg
     9361 gcatcatagt cagaaaattt aaattcaatg tccgtattta cttctggtgt tttatgctta
     9421 ttgtttggta tataaaacca atcaagagta gtgttatcta aagaagatac atctaattta
     9481 ttattatcat tattagtagt ggcttttgat ggtttatctg ttccgtctga aactattaca
     9541 ttggaagtat ctttaccact atctattttt tccttatctt cttgtaactg tgtttcctta
     9601 ttttgatttt cgttttgatt gttttgtgaa tttgaacatc caacaataaa aaaaatagaa
     9661 atagaaagta gtgttaaata atgcaatact tttctaaaca ttttgaagct cctttctaaa
     9721 ttaataataa ttgcatataa taataattat atgattaatt acaattgcat atagtattat
     9781 tataattgga aattactatt aataatagtt atatctttaa aaataaagtc acaatagtat
     9841 gacaaagggt gaataatcaa taaatttaaa aaaaaatgaa aaaaatagtt gaccatatta
     9901 tgagaagatg ttattataat aaatgtgctc aacaagaagt aacaaaataa caagcacaaa
     9961 gaagttaatt gaat
//
LOCUS       DACXMZ010000149         9752 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.150, whole genome shotgun sequence.
ACCESSION   DACXMZ010000149 DACXMZ010000000
VERSION     DACXMZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.150"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            51..839
                     /locus_tag="JD016_13675"
     CDS             51..839
                     /locus_tag="JD016_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAU5000682.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            839..1312
                     /locus_tag="JD016_13680"
     CDS             839..1312
                     /locus_tag="JD016_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAU5000683.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(1404..1844)
                     /locus_tag="JD016_13685"
     CDS             complement(1404..1844)
                     /locus_tag="JD016_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HAU5000684.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(1859..2152)
                     /locus_tag="JD016_13690"
     CDS             complement(1859..2152)
                     /locus_tag="JD016_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAU5000685.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            complement(2195..2596)
                     /locus_tag="JD016_13695"
     CDS             complement(2195..2596)
                     /locus_tag="JD016_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000686.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQIGK"
     gene            2829..4949
                     /locus_tag="JD016_13700"
     CDS             2829..4949
                     /locus_tag="JD016_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAU5000687.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            5174..6340
                     /locus_tag="JD016_13705"
     CDS             5174..6340
                     /locus_tag="JD016_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAU5000688.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLYRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIINPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            6365..7807
                     /gene="nhaC"
                     /locus_tag="JD016_13710"
     CDS             6365..7807
                     /gene="nhaC"
                     /locus_tag="JD016_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAU5000689.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            7804..8079
                     /locus_tag="JD016_13715"
     CDS             7804..8079
                     /locus_tag="JD016_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000690.1"
                     /translation="MNMKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTL
                     LIVIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(8156..9583)
                     /locus_tag="JD016_13720"
     CDS             complement(8156..9583)
                     /locus_tag="JD016_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HAU5000691.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGQLSYGSKVETVEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
BASE COUNT         3402 a         1406 c         1378 g         3566 t
ORIGIN      
        1 aaaatttgat ataatttcaa tattacgttt tttaatttgg aggtgtattt atgaatcaaa
       61 atgaggaact tgaatataag aaaaatattc taaaactagc tctatttgta ggggaactga
      121 tgataaaaaa tggagctgaa acatctaggg ttgaagacag tgtccttaga atatgcaggt
      181 caagagggtt ttatcatgta aatattttta caactcctac agtaataatt atttcagatg
      241 agaaatttga tggcttttca tttatgaaaa cgattcaatc aagaggtata aacttaaata
      301 agatttcttt attaaacagt ttttctcgag aatttgttag tgacaaagag tatgatattg
      361 ataatgccat tgcacgtctt tatgaaatcc aagatgtaaa accttatcct ttatggttat
      421 tttactcttg tacaggaatt gcatctgctt gttttgcttg tttgcttgga gggaattatg
      481 tattaaattt tatacttacg tttataatag caatctttgc tgcaattact tatgataaaa
      541 cgatgaagat aagtgcaata tctgcatttt cctgtttagt atcttctttt ttaattgcac
      601 tttcaggagt tctactagtt gaaataggca ttttagatga ccctaaaatg cttatagtcg
      661 gcgctataat gccactactc ccaggagttg cttttataaa agctattaga gatttaatat
      721 cagggaattt aatatcagga atttctcgtg cttttgatgc tgctatgatt attattgcaa
      781 ttgcttctgg agttggtttt gtccttgata catggtatag aataggaggt cctttctaat
      841 gactgatatg tctatgattt tgcatttttt attttcaggt gttgcaactg ctggatttgc
      901 tgttttcttt aatgctccac tatatctact tcttccagct ggagtaactg gcggaattgg
      961 ttggattgta tatgtttatt tatttaattt tactacaaat gcagtttttg caggttttat
     1021 cgctgctgcc ttggtctcag cgtgcagtga gactttagca agaaaactaa agcaaccagc
     1081 tattgtgttc gtaattccag gtattcttcc tctaatacca ggaattgggt tatataatac
     1141 aatgttgtct ttgattcaga aaaattattc tttggctatg tcaaaaggta cagatgcgct
     1201 atttctaagt gctgcaatag cacttggtgt attagttgta acttcttttg taagaacatt
     1261 gaatcttttg aaaatcagaa aaaactttat tccttacaga aaatcaaaat aaaaatataa
     1321 aaatagcaat gtacttaaac tctacaaata cgtattgagt atttatttaa aaagcacatt
     1381 gctatttttt aattataatt atcttaaagg tcaattaata ttcttgttat tactggtata
     1441 aattctctac tgtctctaaa ttttttgtca ttcttcttta agtataaata acagattatt
     1501 gtaagacaaa ttccaactaa tccacttatg gcttcaactc cagttgtcat tccaattgcc
     1561 cccaatatat aataagtccc tattgttcct acaagaagtg ctataagagg tattatataa
     1621 gccattacag ctgcttttaa tacattcata ttatccatac tcacttctac atggtcacca
     1681 acttttgcac caattgtgtt gtctacttct actaatattt cttttgactc tgctgaattt
     1741 gtagtagcac attttccaca agcagcacaa gctgaatgtc tttgcatttt taactttgca
     1801 gttctatcat caacaatttc cataataaat ccttgttgat tcataactaa ccgcctcctt
     1861 attttgtaaa tttatttata gtatccataa gctcttttat catctcttca gttttatcag
     1921 catcatcatg tttaatgctt tctgctacac atccttttat atgattctct aagataattg
     1981 tgcctactct atttatagca gaccttatag cagatatttg aaccaatata tcaacacaat
     2041 aacgttcttg ttccaccatc ttttgtatac ccttaacttg accttctatt ctttttaatc
     2101 tttttatgag agcttccctc tcattgtttg gtgctaattt tctttcttcc ataaactcac
     2161 ctcatactct aatgatgtct tatacaatta tgctctactt accaatttgt ttaaaatata
     2221 atacagttat tattactata aatgccaaaa ttgatattgc aatagaccta ctttgattct
     2281 tagtttgttc catacttaac tttccattca tcttaaatcc tgccaatggg aataaaaata
     2341 taaccagtgc atcaattcca agtacaactt gggctgcaat agtccattta ctcaaaaagc
     2401 agaatattcc gcccacacct aaaagaacct ctaatatatt tataaactta tatgtaggtt
     2461 tttttgctat atattcttca attttttcct tacattctgc acgacatgga tatatattca
     2521 tattgtcatc atattttata gctaatttat tatttggtga ctttatatcc ctattacagt
     2581 aaagacattt tttcatttta tattctcctc tagtcttcct taaaaaattt tgcctaaatt
     2641 attatacgct ctaatactat tataccaaat agatacaaaa aacaaaaata aaaggaataa
     2701 ttttagctac ttcttttact tcaatgtgta gtctataata aatttataat aataattaaa
     2761 taatattatt taatctaatt taattattat gtaatctaaa ataagtcaaa tttgtatgag
     2821 aggttaaaat gaaacaaatg gaattagcta ttgtaagttt aaaaaaagac gctggagaaa
     2881 tttatgaaaa tcaaataagg caattcttag gtgataatct aaagataaat ttatactcat
     2941 ttgaagaggg aaatttaaag ttttttaaag aaaaacttat actcctatct gcttatttaa
     3001 aatatgatga aatagtaaaa ttatctcact atgatgctca aatcattgtt cccaagttga
     3061 catttgaaaa gaacagtatt gatatgatat ccaaattaga aaaagataaa attatttatg
     3121 tatataactt aagtaaagat atggcaatag aaacaatttc tttaatacat agattaggaa
     3181 tagacaatat aaatatcctg ccctgctatc cagagattga gtttactccc acagatgctg
     3241 taatcctaac tcctggcgaa aaaatcttgc ctaagtttaa aaattgtgaa gttatagatt
     3301 taaaatacag aataatagat ttaagttgta ttgttgaaat tgctacaaaa accaaattaa
     3361 aacatctaat aaaagatgac ctaataaaaa aatacgtaga aaaaataatt cctacaagtt
     3421 atagtacagg tgagttattg ttagatgcaa ataagtttga aagacaattt gatttgttac
     3481 tatctataat tgatgatgga attatatgca caaataacga tggaataatt caattctata
     3541 atcatatggc tagaaaaatt ttgtctataa atgcaaatga aatgattgac tcatttgttg
     3601 gtgattgcat aaaagacatc aattttcaaa gcatattgac caataaaact ccattttttg
     3661 agaaattgat taaaataaat catattgata taaatttaga gattaaacat atacaattaa
     3721 atgtatttga tggatttata ctaaagatga caaagttttc tcaattagaa aaaaagcaag
     3781 ctaaattaag agcacaacta gtaaactctg gtaatatcag taaatacaca tttgatgata
     3841 tattaggttc tagtatacaa actattaata caaaaaaaat tgctaataaa atggctcaat
     3901 caaattcatc tatccttata attggtgaaa gtggtactgg taaagaatta tttgctcaat
     3961 ccattcatag tgcttctagg agaaaagatg gtccttttgt cgcagtaaat tgtagtactt
     4021 ttcaagaaaa tctactgcaa agtgaattat ttggctatga tgaaggggct tttactggtg
     4081 ctaaaaaagg tgggaaaatt ggtttatttg aattagccaa caatggaact atatttttag
     4141 acgaaattgg agaaatggat ttaaattctc aatctaaatt acttagagtc atacaagaaa
     4201 aacaagtacg acgtattggt tctaataatg tcattgacat tgatgtaaga ataattgcag
     4261 ctactaatag aaatttaaaa gaacttgtat ctaaaaatat gtttagaaga gatttatatt
     4321 ttaggttgaa tgttcttccc cttaaaatac atccacttag ggaaagacct gctgatatat
     4381 ttgaaatttt tggttcatta aaatatgata ttccatgtaa ttttattcta agtgaggaag
     4441 taaaagaaat ctttaaaatg tatagatggg aaggaaatgt aagagaatta agaaacttag
     4501 gagaatactt ctgttactta gaaaaagata ttatagaaat atgtgattta cctgaatata
     4561 tattggatac tatagattca aattatagta gaaccgtttg taataaagta agtgataata
     4621 taaaaaagta tcaattcaac attggcaaag acaaaaacat catgaaatac gactataatt
     4681 ttaaaaggtc actagatgaa tacatattta tattggataa tttaaaaaag gcttatgatt
     4741 taaaagaaag aataggtaga aaaagcttat gtaaaatagc tttagaggaa aatagatttt
     4801 tgacagaaca acagattaga aatatgcttt tagaactaca agactttggg cttgtagaca
     4861 tacttgttgg tcgtggaggt tcaattataa catcaaaagg agtagaattt ctaaaaaata
     4921 taaataggtc taacaagtta aacagttaaa cctatttaac ctatttaatt acattttcat
     4981 atatttaaaa atttattaga aataaccttt tccacaaagt tttaagtata attaatattt
     5041 ttatcttatc tatgctctga aattacttaa tattaaatat taacttgtga ataaatattt
     5101 tttattatca atattggcac gttgtttgct taatattatt aacataaaat taaaattact
     5161 ggaggtattt acaatgaatt ataattttaa tgagattgtt gatagaagca ataatttttc
     5221 atctaaatgg tcagaaatgg aaaaaaagta tggaactaat gatttacttc ctatgtgggt
     5281 ggctgacatg gattttaagg ctgcaccttg tataattgat tccttaaaaa atagactaga
     5341 acaagagatt tatggctaca caacaagacc agattcttac aatgaatcta tagtaaactg
     5401 gctatatagg agacataact ggaaaataaa aagtgagtgg ttaatttata gtccaggtgt
     5461 tatcccagct ataagtcttt taataaatga actcacaaaa gcaaatgata aaataatgat
     5521 acaagaacca gtttacagcc cttttaatag tgttgtaaaa aataacaata gagaactaat
     5581 tataaaccca ttacaaaaac tagaaaatgg aaactacata atggattatg aagacattga
     5641 aaacaagatt aaagatgtaa aattattcat actttgtaat cctcataatc cagttggtag
     5701 agtatggaca aaagatgaat taaaaaaact aggagatatt tgtcttaagc ataatgtaaa
     5761 aatcatatct gatgaaatac atagtgacat tatattaaaa aaacataagc atattcctat
     5821 ggcatctatt tctaaagaat ttgaaaaaaa tacaattact tgtatggctc caactaaaac
     5881 ttttaatata gctggtcttc aatcatcata tgtaatacta cctgatgaaa aagactataa
     5941 attgttagat gatgccttta ccagaattga tataaagaga aataattgct ttagcttagt
     6001 tgcaactgaa gcttcatata ataatggtga atcttggcta gaatcatttt tagaatattt
     6061 agaatctaat atagattttg ctataaaata tataaatgaa aatatgccaa aacttaaagt
     6121 tagaaaacca gaaggtactt atttattatg ggtagacttt agtgcacttg gattaagtga
     6181 tgaggaacta gaaagtatac ttgtacaaaa aggtaaagtt gctctaaatc aaggaaacag
     6241 ctttggcatt ggaggaagtg ggtatcaaag aataaactta gcttgtccaa gaagtatgct
     6301 tgaagaagct cttataagaa taaaaaatgc aattaactaa aaatatttaa ggagaatttg
     6361 cactatgaaa atgaatacag aaagaaaaat accttcattt agatattcct tgttagtaat
     6421 gcttgcaata atagccctaa catcagttgg tatagttgta tttaatgctt ctataactac
     6481 aatgttttta ctttcatggc ttatagtagt tcctgcagcc atgaaacttg gttatactaa
     6541 tgatgaaata gaagcttttg gatttgaagt tggtaaagat gcctttcaat caaacttaat
     6601 tatactttct gttggtgtac taatagcagc ttggatagct gctggtacaa taccaacagt
     6661 agtatatagt ggactaacaa taataacccc taaatatttt ctgcttacaa cattaatcgt
     6721 atgttcatta acttctgttg ctactggtac atcttggggg actcttggaa cttctggtat
     6781 agcaatgatg agtattggta ctagtatggg tattcctctt ggtttaacag ctggtgcagt
     6841 catttctggt gctttctttg gagataagat gtcacctcta tcagattcta caaacttagc
     6901 tgcagcagtt tgtaagacag atgtaattac acatatgaag catatgtttt ataccacagg
     6961 acctgcatac gtaatatgta tagttctgta tacagtaatt ggatttaagt actcaaataa
     7021 tactatagac tatgttcaaa taaatcaaat caaggatgta ttaaattcaa actttcatat
     7081 tggtttagta gcaatgattc caataatatt tttattatta cttttattgc tacaaaagcc
     7141 acccattata tctatactaa gttcagccat catgggactt attatagcag tatttcaaga
     7201 aggagaaaaa ataggtgatg ttttaaatta catgctatca ggatttacta tagatactgg
     7261 atttgtctat gccgacaaac tattaaatcg tggtggaata atgagtatgg ctgaaaccgt
     7321 tttactagta tttgtagttt tcgttattgc tggtattctc caaaaaactg gattcttaga
     7381 agtactgctt caacctctta taactaaaat aggaaaatca agaactaaat tggttgggtc
     7441 tacttttata gtaagctact ttgcaaatgc atttagttca tcaatgatgt tcacttctgt
     7501 atttgtcgga actctaatgt ctccaatata caaagaattt aaattaaaac cacaaaatct
     7561 atctagaatt atagaagata cagctacact tggtggtcca ttaataccat ggaattcaaa
     7621 tgctgttttc tgtgctcaga ctcttggtgt aagcccacta gaatttattc catattgttt
     7681 cttaagttgg attacaccta taatttcatt tatttatggt gtaactggtt ttactatgct
     7741 tacatacaca gatgatgaaa ttgaagagtt agaaatattt gatactgctc agagaagagt
     7801 tgattgaata tgaaatttga atactattac ttaattcagg atattgcagg tatattattg
     7861 gcatttatag gacttagaat gtctattatt ggatttagga tactttctat gaaggggctt
     7921 tctataaata cacttttaat tgtaattaaa tactgcttat ttaccatagc aggactgaat
     7981 ttattaatat ctaagtttgg aattagacac tggatttgga gtgtttgtat gttgataata
     8041 tccataataa taaatcccag gattaaggta tcaaaataga aatagttatt agacttaaat
     8101 ttttataaaa aaactgcttt acaaattaaa gcagtttttt tattgttact tataattaaa
     8161 taactggcgt atcagataag taatcattac ttacataacc atatccacca ttatacttta
     8221 tttttaccca tccagtagca aacatttcta ctgtttctac tttgcttcca tatgaaagtt
     8281 gacctatttt ttcatcttct gtacttggtc ctttcctaac atttagacca tctttttctt
     8341 ttacatactt tatttttata ttttcattac tattatcagg cttattattt gtcaaataag
     8401 acccactaac atatccaata ccttcattgt attttatttt tacccatcca gtttttaaca
     8461 catctaaaac ttctacttca tcaccattat ttagatatcc tataacagaa gcttctattg
     8521 tagcatcatt tcttatattt accttatctg tattagttac atacattact ctcttattgt
     8581 tactatttgc ctgtaaatct tttaattctt taaatgcatt ttcatttcct ccactaccaa
     8641 cttgagttat agaggtagca tttttattcg aaaatacact cttttgtgtt gcacttaatt
     8701 gttctcctac gattagtaca ggagaatttt cttttgatgc tagtactcct actgctagag
     8761 cgtctataag ttgattttga tttttcattc catccttagc cacaaataca ttgtttaatt
     8821 tattagatgt ataaaacttt tctattactt taccattagt ttcatttcta tctgctccac
     8881 ctattctttc tgaattaggt aaactttgtt ttattttatc tgatacagca ttagtagagc
     8941 ctattatgta tgatttattt atattcttag attctataaa atctttaaag ctagatatac
     9001 cagatgcatc agatactgga agtataacca ttccattagt agcagctaca gaagctatac
     9061 taactgcatc tgcaagacct gtgtacccat ttactacagc tatttctgat atatctttta
     9121 atccttctat ttttttagct atactcaaag ctgtttggta tctatcacta ccaccaactc
     9181 tatcagtagt taaattatta gattctaact gtttttgcac atcatttgat acagaactag
     9241 atagaccaat tatataaact tttttaactc ctaatctttt taattctttc tcagtttgtg
     9301 aatttagatt atttttttct gtcaataata taggagcatt ttttaattta gcaaatggtg
     9361 ttacagctaa tgcatctaca atagattcac tatttacaac tacagcctca tttgcactac
     9421 tccatccttt tttacttact tcaacagcag tttgatacct atcctgacct actaaagtat
     9481 tctcaactgt tgttctactt atttcatttg catatgctac tggtgataag cttaaaacta
     9541 gagataagca cagcattgat gatattgatt tagttaattt catcttgact ccccctaaaa
     9601 ttatagtatc taaatatttc ctatttaaaa tagtataaca taataattta agtaacatca
     9661 ttattataaa atattatttt tcttatctaa taagctttta ttcttacttg acattaatac
     9721 tttgctcatc aaatgaattt tttatataac ta
//
LOCUS       DACXMZ010000150         9724 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.151, whole genome shotgun sequence.
ACCESSION   DACXMZ010000150 DACXMZ010000000
VERSION     DACXMZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.151"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(402..659)
                     /locus_tag="JD016_13725"
     CDS             complement(402..659)
                     /locus_tag="JD016_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU5000692.1"
                     /translation="MNGLNGKPTEYPENALVPYPVILAASKGDPDAMKMVLQHFSGYI
                     ASLSMRKLYDERGNVYYGVDEDIRERLQARLMRAILTFRAE"
     gene            complement(640..1080)
                     /locus_tag="JD016_13730"
     CDS             complement(640..1080)
                     /locus_tag="JD016_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAU5000693.1"
                     /translation="MTPNRREFEKQCAFQKFCKSVLHNEACNAHEEIRRRRVREVTFS
                     NLALHEERQLYTVDKYFQDEEAEPSYQMAGKEITPKLLLEAIRALPEERRKIVLLYYF
                     EGLTDIEIGKQLNISRSTVQYRRTSSFEQLKKYLEENADEWIEW"
     gene            complement(1543..2163)
                     /locus_tag="JD016_13735"
     CDS             complement(1543..2163)
                     /locus_tag="JD016_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HAU5000694.1"
                     /translation="MLQKIYPRSKDKETVYLKSVITNPNIIVGDYTMYNDFVKEPKDF
                     EKNNVLYQYPINQDKLIIGKFCSIACGAKFIFNSANHTLSSLSTYPFPIFFEEWGLDV
                     KDITKAWDNKGDIVVGNDVWIGYEAVILAGVTIGDGAIIGARAVVTKDVPPYTIVGGV
                     PAKPIRKRFNEETIDILLKLKWWDWSKERIAKNIAAIQSGNVKQLN"
     gene            complement(2175..2885)
                     /locus_tag="JD016_13740"
     CDS             complement(2175..2885)
                     /locus_tag="JD016_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU5000695.1"
                     /translation="MCTRFVYNGNDMITGFNFDIDLAVWKHKVIKEKERFYIGILRPD
                     GTYHSYHGINRNGNVGTLLYVHDNPAGIYRDNSDCITIADLTEQFIKAQISFDEVLQI
                     VKAKQVTYAPDATMQAMLSDSHGRVLIIEPGIGYKEEHEQYSLITNYSLMKPESTKDF
                     IVPGDDRYERALKKLEKYSPDFSISDAIHLLYDVRQEGAWATRVSFVYSTKEQTVYYV
                     ENNHFECIQTFTFPLERY"
     gene            complement(3019..3282)
                     /locus_tag="JD016_13745"
     CDS             complement(3019..3282)
                     /locus_tag="JD016_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015544809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000696.1"
                     /translation="MKYKNAQAILPDALVKELQSYVQGEYIYIPVEQEQQKRWGEVSG
                     YRKELEQRNQKIKQEYQSGISINCLAEKYCLSLYAVRKIIYQK"
     gene            3587..3952
                     /locus_tag="JD016_13750"
     CDS             3587..3952
                     /locus_tag="JD016_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5000697.1"
                     /translation="MIMKQNKEKFDFKAFGQAIKAARKAKGISRNQLADTLNIAPRYI
                     ASIENSGQHPSLQILYELVTLLDVSVDQFFFPEREQEKSTRRRQLDTMLDTMSETDLK
                     IMSATAKGIEEANNDRTGE"
     gene            4195..4524
                     /locus_tag="JD016_13755"
     CDS             4195..4524
                     /locus_tag="JD016_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAU5000698.1"
                     /translation="MNGRKRKVQIKFYVTEEERALIEQKMKLVPTRNMAAYLRKIAID
                     GYIIQTDHSDIKAMTTEIQKIGVNINQIAKRVNATGSVYQEDIGEIKGVLAEIWRLQR
                     LSLLKAR"
     gene            4485..5831
                     /locus_tag="JD016_13760"
     CDS             4485..5831
                     /locus_tag="JD016_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAU5000699.1"
                     /translation="MAVTKIKPVKSTLSKALDYIQNPDKTDGKTLVSSFGCSYETADI
                     EFGYTLSQARQKGNNLAFHLIQSFEPGEVDCEKAHAIGKQLADAVTKGQHEYVLTTHI
                     DKGHIHNHIIFCAVNFVDYHKYNSNKRSYYGIRNMSDKLCRENGLSVVVPGKGSKGKS
                     YAEYQAEKAGTSWKGKLKIAVDTLIPQVASFEELLQRLQAAGYEIKPGKYISCRAPGQ
                     ERFTRLKTLGADYTEEAIKERIAGKRRKTAKVPKEQRGVSLLIDIENSIKAAQSRGYE
                     QWAKIHNLKLAAKTMNFLTEHQIEQYADLVSRIEEINAENEKTAEALKSVEKRLADMA
                     VLMKHVATYQKTKPVYEAYRKAKDKDTYRAKQESNLILHEAAVKTLKAAGVTKRPNLA
                     AIQEEYGKLQAQKEALYADYGKLKKQVKEYDVIKQNIDSILRQPREPEREKGKERE"
     gene            6011..6421
                     /locus_tag="JD016_13765"
     CDS             6011..6421
                     /locus_tag="JD016_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009265988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5000700.1"
                     /translation="MNIADRIQYLRKQNGYSQEELADKVGVSRQAVSKWESEQSTPDL
                     EKVIIMSELFEVTTDYILKGIEPVSTTNRKTIRTLYVGFALIFAIIAGIWSFAANRFN
                     YDEIILITLAGGVVGFGIALIVQVIGGMISNKKQ"
     gene            6477..7133
                     /locus_tag="JD016_13770"
     CDS             6477..7133
                     /locus_tag="JD016_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000701.1"
                     /translation="MNKFLNDIRSAENPISSNRKIINTIAVLFLGIALGTFSKFLDFR
                     QVELPSVLMAIDGALDVHNFLGRFAIWVLIALCISIYSNSAIRASVNVFAFFVGMVTS
                     YYLYSNYVAGFFPRSYAMIWFGFTIISPFLAFVCWYAKGKGKLAFMLSILILAVLFNM
                     TFVYGWGYFEALSVLELAVFIIGLTVLRRDTLRSSVLMGTISIVLAFLLDMVIPFHFG
                     "
     gene            7173..7403
                     /locus_tag="JD016_13775"
     CDS             7173..7403
                     /locus_tag="JD016_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU5000702.1"
                     /translation="MEQEMPDYETIRAAVTGEKWALEKVLDCYGDEINHLAMVKKRQP
                     DGTTKEEIDEDLRQTLILKLLEAIPQFPIERE"
     gene            7408..7776
                     /locus_tag="JD016_13780"
     CDS             7408..7776
                     /locus_tag="JD016_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000703.1"
                     /translation="MTRGEIWKDFFKRTVLPAVIALFLFFMFKNVFTEDGQTNYFYVW
                     LCCGIPFGIRRMFVWLVPHGYDIAGTVGIVALNFILGGIIGGVILIWRLVVATWYIPL
                     TIYRLLTADKGATPQINMEK"
     gene            complement(7954..8319)
                     /locus_tag="JD016_13785"
     CDS             complement(7954..8319)
                     /locus_tag="JD016_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU5000704.1"
                     /translation="MTGVKRLTPPQSRKVNSLVKKECCNCERGHCILLDDGEECVCPQ
                     LISYSLLCKWFQIAVLPLDKLLYAELFKTEDKKRCSECGTFFVSKSNSVKYCPDCRKR
                     ITRRQAAERMRKRRAAVTQ"
     gene            complement(8316..8522)
                     /locus_tag="JD016_13790"
     CDS             complement(8316..8522)
                     /locus_tag="JD016_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAU5000705.1"
                     /translation="MIRLTVEEMNLLSIYHEGSKVQLIENMTAALPFMDEDMRPFAER
                     TLQKISPLTENEYAELAIFAADEV"
     gene            complement(8525..>9724)
                     /locus_tag="JD016_13795"
     CDS             complement(8525..>9724)
                     /locus_tag="JD016_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAU5000706.1"
                     /translation="FATWALSNNSAEIRRVKDRIKSLSQQKEIGFVGWEFDGGKVDAN
                     TEANRLQIFFEDKPDETTREALKSNGFRWSPKAGAWQRQLTSNAYYAADYIKAIALLT
                     GEKPTELQRAHIRAQKVAALEQPAQGQPEQEAPQDKDTFSIYQIKGGDETRDLRFEPY
                     DRLTAAGYRVDVKNYALVYSAPLTPGTSLEDIYTRFNIDHPKDFKGHSLSVSDVVVLH
                     QNGQDTAHYVDSFGYKEVPEFFKEQEKALIPDELETGETIKTPRGTFYVTAMSRAQME
                     AAGYGLHHQSDDGKYLIMGNGTRAFAIAAEQPENYLKTAEQTTEQNYNMIDGQINNTP
                     TAAELEEKAKAGGQISLAEYAEALKAEKKQTEPEKKPSIRAQLRADKEKTAQKKAAKT
                     KNHELEV"
BASE COUNT         2726 a         2054 c         2121 g         2823 t
ORIGIN      
        1 aatgggtcaa aagtcaattt gtaacatagg ctttaatcgg acggctatta gcgtaacctc
       61 atgggacttg cacccctccg gcggtctgcc gaagccctac ccattgcctg cgacgcttct
      121 aacgctcgga ctatggctat aaggaagtat cattatgtat tctgtgtgtt atcgcccgca
      181 ggctgctatc cctgctttac ggtgtggtat ttctcgctcg cttttctatt aagagaaagg
      241 tcatggcgta cccaccgcat ggctcggcac aaaaggaacg tatccgattt gccttatgct
      301 gcgtcgccgt tctattgcgc cgctttcttt atcaaagagc agggggctgt cttgcggtat
      361 ggaacggaaa aggggaaaga agcgttccaa tatcgcgtag gttattcagc ccgaaaagtg
      421 aggattgccc tcatcagtct tgcttgcagc cgttcccgaa tatcctcgtc aacgccataa
      481 tagacattgc cgcgctcatc gtagagcttc cgcatggaaa gacttgctat gtagccgctg
      541 aaatgctgca agaccatttt catagcgtcc gggtcgcctt tgctcgccgc caaaataacg
      601 ggatagggaa caagggcatt ttccgggtac tcggtcggtt taccattcaa tccattcatc
      661 agcgttttcc tccaaatatt tttttaactg ctcaaaggag cttgtccggc gatactgtac
      721 cgtacttctt gagatattga gttgctttcc gatttctata tcagtcaatc cctcgaagta
      781 atacagcagt acaattttgc gccgttcttc gggtaaagcc cggatagctt caagcagcag
      841 ttttggggtt atttcttttc ctgccatttg ataagacggc tctgcttctt cgtcttgaaa
      901 atacttatcg acggtataaa gctgccgttc ctcatgcaag gcaaggttgg aaaaagtaac
      961 ctcccgaact ctgcgccgac gaatttcttc atgggcgttg catgcttcat tgtgcagcac
     1021 tgatttacag aacttttgaa acgcacattg cttttcaaat tccctgcgat taggtgtcat
     1081 ggtgttcacc tcctttctgc cgaaaggcgg tagtacctcc ccttttcgcg ggataataca
     1141 ggcgactttt tgctttgcca aaaagaaggc aaagtttttg aaaaatcttc tgtaaaatgc
     1201 gaaatgcgcc tgcctgcaaa atgcagacag acgcatagga aataacagca gtatttaatt
     1261 gatttaacag ttcatctaca tagccggaat aacccgttgt aggatacaaa ctttttttga
     1321 caaaagacgt ccttttgtca gataagcata acttatttta ttcagcataa cggcaacctc
     1381 ctatataccg ccatgctgat tgcaaggtca aatgaggtcg tcgttgctaa atttatgcaa
     1441 aaagaagcgg cggctctgaa attcaaaacc accgctgaaa aaacgcatta aaagatgtct
     1501 gcattgcgac ggcttttttt cttttgccgt cgcgcggcag atttagttta gttgtttcac
     1561 attaccagat tgaatggcag caatattttt agctattctc tctttagacc aatcccacca
     1621 ttttaatttg agcaatatat caatcgtttc ctcattaaat ctttttcgta taggttttgc
     1681 aggtacaccg cccacgattg tataaggtgg aacatctttc gttacaacgg ctcgcgctcc
     1741 gataatagca ccgtcgccaa tcgtaacgcc cgctaaaatg actgcttcat aaccaatcca
     1801 aacatcattt ccgacaacaa tatctccttt attatcccat gcctttgtaa tgtcttttac
     1861 gtctaatccc cattcttcaa aaaaaatagg aaatgggtat gttgataagg acgataatgt
     1921 atgatttgca ctattgaaaa taaactttgc tccacacgcg attgaacaaa atttcccaat
     1981 tatcaatttg tcttgattga tagggtattg atataaaaca ttattttttt caaagtcttt
     2041 tggttctttc acaaaatcat tatacatagt ataatcgccc actatgatat tagggttagt
     2101 gataacactt ttaagatata ccgtttcttt atcttttgaa cgtggataaa ttttctgtaa
     2161 cataatggtt cctcctagta tcgctctaac ggaaaagtga atgtttgaat acattcaaaa
     2221 tggttatttt ctacataata tacggtttgc tctttggtag aatacacgaa agaaactctc
     2281 gttgcccatg cgccctcttg acgtacatca taaagtaaat gaattgcgtc tgaaattgaa
     2341 aaatcggggc tatatttttc taattttttc aaagcacgtt catatctatc gtcccccgga
     2401 acaataaaat cttttgtact ttccggcttc atcaaagaat aattcgttat caaggaatat
     2461 tgttcgtgtt cttctttata gcctattccc ggttcaatga ttaaaacacg tccatgactg
     2521 tctgataaca ttgcctgcat ggttgcgtcc ggtgcatagg taacttgctt tgcctttaca
     2581 atttggagta cctcatcaaa agaaatttgt gctttaataa actgttctgt caagtcggca
     2641 atcgttatac agtcagaatt atctcgatat attccggcag gattgtcatg cacatataac
     2701 aatgtcccaa catttccatt cctattgata ccatgataag agtggtatgt accgtcgggt
     2761 cgtaaaatac ctatataaaa gcgttctttt tcttttatca ctttatgttt ccacactgca
     2821 aggtcaatat caaaattaaa tcctgtaatc atgtcattcc cattataaac aaatcgggta
     2881 cacataatag ttccctccca tgtattttaa atatttcttc tttatcatac agttcgttga
     2941 aaaatttttc tatactctat ataataccga accaatctgt ataaaaataa gaggctgcac
     3001 actcggcagc cccttacctt acttttgata tataattttc cgaacagcat ataaagatag
     3061 acaatatttt tcggcaagac aatttataga aataccgctt tgatattcct gttttatctt
     3121 ttgattacgt tgctctaatt ctttcctgta accggaaact tctccccaac gcttttgttg
     3181 ttcctgttcg acaggaatat aaatatattc tccttgaaca taactttgta actcttttac
     3241 caatgcgtcc ggaagaatag cttgtgcatt tttatatttc atgcaggctt cctccttgat
     3301 tatgatagtc aagttacaaa gccagcattt attctgcgac tattttactc catgcaaccg
     3361 tcgcaatcta ctggctttgc atggagaaaa tctttgtttc tctttttggt gttgatacac
     3421 atataaatca cttctttcaa tcaaataggg attattgtca gtataacatt ttcatctcaa
     3481 aaagacaacg ctgcttttaa tataatatgc tctatttcta tccatactat gatatgtgta
     3541 atcatatctc aaagaaaata ggaacagtaa aatgtaactg ggtgaaatga ttatgaagca
     3601 gaataaagag aaattcgatt ttaaggcttt cgggcaagcc attaaagcag caagaaaagc
     3661 aaagggaata tcacgcaatc agttagccga cacgctgaat attgcgcccc gatatatcgc
     3721 gtccattgag aacagcgggc agcacccaag tttacagatt ttatatgaac ttgtaaccct
     3781 gcttgatgta tcagtagacc agttcttttt cccagagcgg gaacaggaga agtccacacg
     3841 ccgccgtcag ctcgatacca tgcttgacac aatgagcgaa acagacttaa aaatcatgtc
     3901 tgctactgca aaaggcatag aggaagccaa taatgacagg acgggggaat aaagttccct
     3961 cgtttttgtc tgcataaagt gattgaagcg tcgcaggatt gcggcgtttt cttttgcgct
     4021 tttttggcat actgggaatt tcgttgtagt ggtaagtgag gaaaaaactt tcttagcaag
     4081 cataggaaaa gagtaccctt ttccgtattt tcgtcctccc gccctgtggt gcgtcggttg
     4141 aaaattgctt gttgggaagt gtcccaaacc ctcggaacag aaaggagtga ttgcatgaac
     4201 ggacgcaaaa gaaaagtaca aatcaaattc tatgtaacag aggaagaaag ggcattgatt
     4261 gagcagaaaa tgaagctcgt ccccacccgg aacatggcgg cgtatcttcg caagattgcc
     4321 attgacgggt atatcattca gacagaccat agcgatataa aagccatgac aacggagata
     4381 caaaaaatcg gggtcaatat caatcagatt gcaaagcgtg tcaatgcaac aggcagcgtc
     4441 taccaagagg acatagggga aataaagggg gtgcttgcgg agatatggcg gttacaaaga
     4501 ttaagcctgt taaaagcacg ttgagtaaag cccttgacta tatccaaaac ccggataaga
     4561 cagacgggaa aacgcttgtg tcctcctttg ggtgcagcta tgaaacggca gatattgagt
     4621 ttggatatac cttgtcgcag gctcggcaaa aaggaaataa cttagccttt catctgatac
     4681 aatcgtttga accgggagag gtagactgtg agaaagccca tgcaatcggg aagcagcttg
     4741 ccgacgcagt aacaaaggga cagcacgaat atgtcctaac cacccacata gacaaggggc
     4801 atatccataa ccatattatt ttttgcgctg tcaatttcgt tgactaccat aagtacaatt
     4861 ccaataagcg cagctactac ggcatacgga acatgagcga taaactgtgc cgggaaaacg
     4921 ggttatccgt tgtcgtaccg ggaaaaggca gtaagggaaa aagttatgcg gaatatcaag
     4981 ctgaaaaagc cgggacaagt tggaaaggca agctgaaaat tgccgttgat acgcttatcc
     5041 cccaagtggc aagttttgaa gaattgttgc agcggttgca ggcggcgggc tatgagataa
     5101 agccgggaaa atatatctcc tgccgcgctc ccggacagga acggtttacc cgcttaaaaa
     5161 ctctcggtgc agattataca gaggaagcaa tcaaagaacg gattgcaggc aagcgcagaa
     5221 agacggcgaa agttcccaaa gagcagcgcg gcgtatcgct tcttatcgac attgaaaaca
     5281 gtattaaggc agcgcagagc cggggctatg aacagtgggc gaaaatccat aatctgaaac
     5341 tggcagcaaa gactatgaat ttcttgacgg aacatcagat tgagcagtac gcagatttag
     5401 tcagccggat tgaagaaata aatgcggaaa atgaaaagac agcagaagca ttaaagagtg
     5461 tggagaagcg tcttgcagac atggcggtgc tgatgaagca cgttgccacc tatcaaaaga
     5521 caaaaccagt ctacgaagca taccgcaagg caaaggataa ggacacatac cgggcaaagc
     5581 aggaaagcaa tctaatactc catgaagcgg cagtaaagac cctgaaagcg gcgggcgtta
     5641 ctaagcgccc caacctcgcc gctatacagg aagaatacgg gaagctccaa gcgcagaaag
     5701 aagcccttta cgctgattat ggcaagctga aaaaacaggt taaggaatac gacgttatca
     5761 aacagaacat agacagcatt ttacggcagc caagagagcc ggaacgggaa aaaggtaaag
     5821 aacgtgagta gctttgttca tacttatgtg atataatagt tcatatcaaa gaagtttcgt
     5881 agtacgacaa atttgaaatt ttccgaatag cctaaatgaa accgttggtt gacacttttc
     5941 caagcatagg tggtggtaat gcaactataa aaaatgctat gattttgcaa aagggaaagg
     6001 agaattgacg atgaatatag cagacagaat acaatattta cgaaagcaaa acggatactc
     6061 acaagaggaa cttgctgata aggttggcgt atcaagacaa gctgtatcaa aatgggaaag
     6121 tgaacaaagc acaccagatt tagaaaaagt aattattatg agtgaacttt ttgaagtcac
     6181 gactgattat attcttaaag ggatagaacc agtatcaaca acgaatagaa agactataag
     6241 aacattgtat gtaggctttg ctttaatatt tgcaatcata gcaggaatat ggtcttttgc
     6301 tgcaaataga tttaattatg atgaaatcat tctaattact ttagcaggtg gtgttgttgg
     6361 ttttggaata gctctaattg ttcaggtaat aggtggtatg atttcaaata agaaacaata
     6421 gtgaaaacga aaattccagt ttgtagaact ggaaaatttg aattttggag atgcctatga
     6481 acaagttttt gaatgatata agaagtgcag aaaatcccat atctagtaat agaaaaatta
     6541 taaataccat agctgtattg ttccttggaa tagctttggg gactttttca aaatttttgg
     6601 attttcgtca agttgaactt ccaagtgtgc ttatggcaat agatggagca ttagatgttc
     6661 ataatttcct tgggcgtttt gcaatttggg tattgattgc actgtgtatt tctatttata
     6721 gtaattctgc aataagagca agcgtcaatg tttttgcatt ttttgtcggt atggttacaa
     6781 gttactattt gtattcaaac tatgttgcag gttttttccc aaggagctac gctatgattt
     6841 ggtttggctt taccattatt tctccgttct tggcatttgt ctgttggtat gcgaaaggga
     6901 aaggcaaact agcatttatg ctatcaatat tgattttagc agtattgttt aacatgactt
     6961 tcgtatatgg atggggatat ttcgaagcac tctctgtttt agaattggca gtctttatta
     7021 ttggacttac tgttttgagg agggacacat tgagaagttc tgtgctaatg ggaacaatta
     7081 gtatagttct tgcattttta cttgatatgg tcattccatt ccattttgga taaacaactt
     7141 acagtttgtc aaataaaagg aagtgtcaga gaatggaaca ggaaatgccg gactatgaaa
     7201 cgatacgcgc cgccgttacg ggcgaaaaat gggcgttgga aaaagtgctt gattgttacg
     7261 gcgacgaaat caaccacctt gcaatggtaa aaaagcgtca gccggacgga acgacaaaag
     7321 aagaaatcga cgaagattta cggcaaacac tcatactgaa actgttagaa gctatcccac
     7381 aattcccaat cgaaagggag tgataccatg acacgcgggg aaatttggaa agactttttc
     7441 aagagaactg ttttgcccgc cgttatcgca ttgtttttat tcttcatgtt caagaacgtc
     7501 tttacagagg acgggcagac caattacttt tatgtatggc tgtgctgcgg tattcccttt
     7561 ggtattcgcc gtatgttcgt atggcttgta ccgcatgggt atgacatagc cggaacggtg
     7621 ggtatcgtcg ccctcaactt tattttgggc ggcattatcg gcggggtcat tttgatatgg
     7681 cggcttgtcg tggctacatg gtatatcccc cttacgatat atcgtttact taccgcagat
     7741 aagggcgcaa cccctcaaat caacatggaa aaatagcaaa gaaaaaaccg ggacgcagac
     7801 agtcagtaaa gactatccgc gccccggttt ccttatgggt gcagttatga aatgttacgc
     7861 agtagaaccc cgatttcaag ggaaaaggta ttctgtatcg cctataccag tttcacgccg
     7921 tttatgccct gtaaatcaag gcttttttcg cccttattgc gtaaccgccg cgcgccgttt
     7981 cctcatgcgc tcggctgcct gtctgcgggt aatacgcttt cggcagtccg ggcagtattt
     8041 gacgctgttt gattttgata caaagaatgt gccgcactca ctacaacgct ttttgtcctc
     8101 ggtcttgaat agctctgcat acagcagctt atcaagagga agtacggcaa tctgaaacca
     8161 cttgcataaa agtgaatagg aaataagctg cggacagaca cattcctccc cgtcgtccag
     8221 taagatacag tgtccgcgct cgcagttaca gcactccttt ttcacaaggc tgttgacttt
     8281 ccggctctgt ggcggcgtga gccgcttaac cccggtcata cctcatcagc ggcgaaaatg
     8341 gcaagctccg catactcgtt ttctgttagt ggggagattt tttgtaaggt gcgctctgca
     8401 aaggggcgca tatcctcgtc catgaatggc aacgcagccg tcatattttc aataagctgt
     8461 accttgcttc cctcatggta aatactcaac aggttcattt cttcaacggt cagtctaatc
     8521 atgctcatac ctccaattcg tgattttttg tttttgccgc ttttttctgt gcggtctttt
     8581 ctttgtctgc tctaagctgc gcccggatag agggcttttt ctccggttct gtctgtttct
     8641 tttctgcttt gagggcttcg gcgtattctg caagggaaat ctgtccgccg gcttttgcct
     8701 tttcctccag ttccgcagcg gtcggggtgt tatttatctg cccgtcaatc atgttgtagt
     8761 tttgctcggt ggtctgctcg gcagttttca gatagttttc cggttgctct gcggcaatgg
     8821 caaacgcccg cgttccgttc cccataatca gatattttcc gtcgtcggac tggtggtgca
     8881 atccatatcc ggcagcttcc atttgcgcgc ggctcatggc ggttacatag aaagtccccc
     8941 tcggcgtttt gattgtttcg cctgtttcca attcgtcggg aatgagggct ttttcctgct
     9001 ccttaaaaaa ctccggtact tccttataac cgaagctgtc tacatagtgg gcggtgtcct
     9061 gtccgttctg atgaagcacc accacgtcgg aaacggaaag gctgtgtccc ttaaaatctt
     9121 tcgggtggtc gatattaaag cgggtgtaaa tatcttcaag ggaagtccct ggcgtaagag
     9181 gtgcggaata gacaagcgca tagtttttca cgtctacccg gtatcctgcg gcagtcagac
     9241 ggtcgtaagg ttcaaagcgc aaatcccttg tttcgtcccc gccttttatc tggtaaatgg
     9301 aaaaggtgtc tttgtcctgt ggggcttcct gttccggctg cccctgtgcg ggttgttcca
     9361 gtgcagctac cttttgcgcc cggatatgcg cccgctgtaa ctcggtcggc ttttccccgg
     9421 taagaagtgc aatcgccttg atataatcag ccgcataata ggcgttactg gtaagctgcc
     9481 gctgccatgc cccggctttt ggcgaccaac ggaagccgtt gctttttaag gcttcccgcg
     9541 tggtttcgtc cggcttatcc tcaaagaaga tttgcagtcg gttcgcttcg gtgtttgcgt
     9601 ccaccttgcc gccgtcaaat tcccaaccca caaaaccgat ttctttttgc tgtgaaaggg
     9661 atttgatacg gtctttcacg cgccggattt ctgcgctgtt gttagataac gcccaagtcg
     9721 caaa
//
LOCUS       DACXMZ010000151         9710 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.152, whole genome shotgun sequence.
ACCESSION   DACXMZ010000151 DACXMZ010000000
VERSION     DACXMZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.152"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            66..740
                     /locus_tag="JD016_13800"
     CDS             66..740
                     /locus_tag="JD016_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000707.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            764..1768
                     /locus_tag="JD016_13805"
     CDS             764..1768
                     /locus_tag="JD016_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU5000708.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFVSYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            1880..3109
                     /locus_tag="JD016_13810"
     CDS             1880..3109
                     /locus_tag="JD016_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAU5000709.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            3117..3791
                     /locus_tag="JD016_13815"
     CDS             3117..3791
                     /locus_tag="JD016_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000710.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            3794..5026
                     /locus_tag="JD016_13820"
     CDS             3794..5026
                     /locus_tag="JD016_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000711.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(5312..6577)
                     /locus_tag="JD016_13825"
     CDS             complement(5312..6577)
                     /locus_tag="JD016_13825"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAU5000712.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(6630..7778)
                     /locus_tag="JD016_13830"
     CDS             complement(6630..7778)
                     /locus_tag="JD016_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HAU5000713.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(7878..8423)
                     /locus_tag="JD016_13835"
     CDS             complement(7878..8423)
                     /locus_tag="JD016_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HAU5000714.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(8646..9560)
                     /locus_tag="JD016_13840"
     CDS             complement(8646..9560)
                     /locus_tag="JD016_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAU5000715.1"
                     /translation="MKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTPLQIITLRFFL
                     AAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTPSKNAFITAAN
                     VVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFLTLICSFGFAF
                     HIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGYMGTMYLAVFS
                     TTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLVIGSILIFVAI
                     ITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         3453 a         1501 c         1303 g         3453 t
ORIGIN      
        1 atattttaat ttatcaagta gaatatagtt atgttaatat aactattttg aattaggagg
       61 taagaatgtt taaaataatg gtagttgaag atgacgtctc attgaaaaat atcatagcaa
      121 agtgtcttac taaatgggga catgatgttc atcaaataga aaatcttgaa aatataatag
      181 aagagttcaa aaactataac ccagaattag ttttattgga cataaacctt ccattttatg
      241 atggttttca ttggtgtaat gaaataagaa aaatttcaaa agttcctata atttttattt
      301 cttcaagaaa ttctaatatg gatgtaataa tgggtgtaaa tttaggtgca gatgattata
      361 tacaaaaacc tttttctgta gatgtcttag tagctaaagt taatgccctt ttaagaagaa
      421 cttataattt tgttgataat aactcaaatc agattattca caatggtgtt actttagatt
      481 tatcaactgc aacaataaac tatgaagata atactattga gctaacaaaa aatgaaatca
      541 aaattctcca tgagcttatg aaatataagg ggcagatagt aagtaggaat aaactcatga
      601 aaaaattatg ggataatgat tggtttgtag atgataatac attaactgtc aatgtaaata
      661 gaatacgctc aaaattaaat gaaattggtt tagaagattt tatagaaaca aaaagaggat
      721 tgggttatat aatcagttaa aacaattcta agtaggtgaa tttatgagct ttaaagattt
      781 tctcaaggat aaactaggtt ttttagtata caattttact ttcttaattt ttacagtatc
      841 agttattata ttttctcctg tagatgtttt tctgactgat actattttat atatattagt
      901 tgtaaatgtt gtgtttttat ttttatatct atttgttagc tacatcaaga agaacaaatt
      961 tttagataag ataaaaaatg atacattcaa aattaatatg aatgatatag aattagctag
     1021 atgtaaaaat gaagaaaaaa tatacttgaa cattattaaa aatcatcaat atcaatgcaa
     1081 taacataata aataataata tcgagaaatt tgaagaaaac aaagagattt taaatatgtg
     1141 ggtacatgat gtaaagatac ctatagctat aataaaactc attcttgaac aaaatgaaaa
     1201 tttaattgat gaaaaaattt cagatgacat agatagtgaa atatttagaa ttgaaaactc
     1261 cgttgaaaaa attttatatt tatcaagact cgaagatttt cataaggatt ttttaattca
     1321 agaggtaaat attgaaaaaa tcattagaaa tattataaaa aaatactcaa aatattttat
     1381 ttcaaaaaaa attattttag acttaaataa tttgaatttt aaggttttat ctgatgaaaa
     1441 atggctatac ttcatattcg agcaattatt gagtaattcc ctaaaatact cctcattagg
     1501 agataatata tctatctatt gcaaaagaac caaaaattat ttacaattaa gagtggaaga
     1561 tactggttgt ggtataaaaa aggaagattt aaatcgaata ttcaataagg gatttacagg
     1621 taataatgga agaaacaatt ctaaatctac aggtcttggg ctttatttag taaaagaact
     1681 ttgtgataag cttggtcata aaattgatgt agattcagag tataaccaat atacaaaatt
     1741 tactattaca ttcaaatgtg atttatagta tcttattatt gatagtttat tttcattaac
     1801 ttatattcaa cttcttatag taattctata tgtacatccc agtatataat taagtagttt
     1861 ttataaaaag gaggaagaca tgtcttttct aaaaaaattt aaaaataaaa gatttagaaa
     1921 taacagtttt aaaagtaaca actttagaaa tagcagtttt ggaagtagtg gcttcaagtc
     1981 taaaaataaa ggttttaaaa acaatccctt taaaaataag agattcaaaa tatatgcagg
     2041 aatagcagta atcatacttt taattctagg agttcttgct tatgttgatt cgaaaaacac
     2101 tgaattacaa gctaatgaaa attttatcga aacatatact attcctgaaa atgaaaaaat
     2161 atttataaat gggatggttg ttcctaagca aactaaggac ttcaatatat ctggagatta
     2221 tgagttaagt gatgttaatg ttacaaatgg tcaaaaagta aatcaaggag accttctttt
     2281 tactgctaaa aatccaacta taatagcaga aatagatagt ttaaaatcac aacttagtca
     2341 atacaaaaaa caaaaaatat cactttctga tattgctgaa aataaagatg ctattgcttc
     2401 tataaatgca cagataactg ctttaaactc tcagatagct tctctggata aaaaagctta
     2461 tgacagagtt actgctcctt ttgatggcac agtatacttg aatgaccaaa ctggaaatcc
     2521 agaccaacca gtttcattta tgactattca aggtctagag ttttatatga aaggacaagc
     2581 aagtgaacaa gatttaccta aaatgaagat tgaccaaatg gtcaatatac tcgttttttc
     2641 aactgaccaa aagcttactg gtagaataag ttttatatct gataaaccat ctactccaaa
     2701 tactgaaatg ggagctcagc agaatacact atcttattat gatataaaca tagcatttga
     2761 taatcaagaa ggattagtaa atggattcca tcttcaagca tcacttgaag tagctaattc
     2821 aagctttaaa ataccagcat cttgtgtact taaagacaac aaacactcat atgtatttaa
     2881 ggatttagat ggtatattga aaaagcaaat tgtagatgta gcaagtcaaa atgatgattt
     2941 tgctgtagtt agaggcggtc ttgaacaagg cgatataata ataaaacacc ctactaaaga
     3001 aatgaaagaa ggagacccag ttcaaggcga tggcgtaact gctggttcta ataatggaga
     3061 tactactcca aacaaaaaag tagaagaagt tactatggat gtgaattaat ttacatatgt
     3121 taattaaatt agaaaatatt caaaaatatt acaaagtagg gaaagatgaa ttacatgttt
     3181 taaaatcctt aaatttagaa attgaatctg gagaatttgt catgataatg ggtaagtctg
     3241 gtagtggtaa aactacttta cttaatatct taggttttct agatgtattt gatgaaggaa
     3301 gatatatatt tgatggaaca gatgtaacta atttgagtga aaatgaacgt tctgttttta
     3361 gaaatattaa tattggattt gttttccaac aatttaattt aatagaaact ctaaatgtat
     3421 atcaaaatgt agagttgcct ctaatttaca ataaagcact aaaaaaatct aatagagaag
     3481 aaattgttaa agataaatta agttctgttg ggcttttgga caagcttaag cagaaaccac
     3541 ttcaattatc tggtggtcaa caacagcgtg tagccattgc acgttgccta gcaaatgacc
     3601 ctcagataat ctttgctgac gaaccaactg gggcattaga tagtgaaact agcagagaaa
     3661 ttatggagct tttaacaagg ctcaacaaac aaggtaagac tataataatg gttacacatg
     3721 atcaagattt aactaagtat gcaactaagg ttatacgact taaagatggt gtgtttactt
     3781 cggaggtgta atcatgagtt tattaaaaaa ttctttggcc aatttaaaag gtcacaagct
     3841 tcgtgtattt gtagctttac tctggattat aataggaata acttcagtaa tacttgttag
     3901 ttctataggt aatggatttc aaaaagaaat taagaaatct gtaaacaatg taaaccctaa
     3961 taaaactact atttcgtttg aatcagccga taacacaggt cttactgatg atatgagtat
     4021 ttttttaaaa ccctttaatg caaaagattt agaagaatta tcatttgttg aaggagtaga
     4081 aagaatagct ccttcaagag atgggtttaa ccttgattca gtttactctt cacaagcatc
     4141 ttttgataaa aaaactacgt atgttgatgt tggaccagta aaaaaagatt ctaaaataaa
     4201 tttaatttgt ggtagagatt tttctttaga tgatgaaaaa agaaaagtaa ttttacttac
     4261 attacaaagt acaagtgaaa tttttgaaaa tccagaagat gccttaggtc atggtatcaa
     4321 cataaatgga actatatttg aaataatagg ggtattagat gattctcagc aaaaccaagc
     4381 tggaggattc tttggaggtt accaagatat gcaatttact acttccctag tacctaaaaa
     4441 ggcttttgac actttaatga gtcaaaattc ttattcaaat gaaatttacc agttagactt
     4501 agtttcttct aaaggctata atgtaaatga agttgcaaac aatgttatag ctaaattata
     4561 tgagatgcac cctggtatca atggttctta tactacacca gacccaactg aacagacagc
     4621 ttatcttgaa agcattaatt ccaatgtaaa caaatatgta tctataataa ctgtagttgc
     4681 aatgtttgtt ggtggtattg gtgtaatgaa tataatgtat gtatctgtta tggaaagaca
     4741 aagagaaatt ggtataagac gtgctattgg agcaaaacct aggtctatat tatttcaatt
     4801 tttagttgaa gctgttttta taacagtatg tggtggaatt ttaggaacta tagttggatt
     4861 tgctgctact aactatgttt ctaaatatat aggttttgaa gctataccta gcttaaatag
     4921 cttattttat gcaattgtag ctactatatt gactggagtt gtatttggtc tgattccagc
     4981 ctttaaagcc tctaaactag accctataaa ggcaatttat aagtagaaat tgtataaaga
     5041 ttttcgattt ttagattagt ttatataaaa catacttaaa tatataacgc actattatat
     5101 atgatagatt aggaacttaa tctttccaaa tataatagtg cgttctttca taaaaaggat
     5161 tctcttcctt actatatcta aattaccaag atgttatgtt ctaattgttc aaactaatac
     5221 ttccaatttt gattaatcaa tctcattctg ctaatataca atcttttact ttcaatcatt
     5281 gtacactccg ccttaaaaaa ataatcttaa attaggcgtt atatacaatc tccccattac
     5341 atattgtata attaaccttt ccatataaag atttaccttt aaatggtgaa ttatcagctt
     5401 tagaaacaaa gctttcaact gtccattctt cttctggatt gaatataaca atgtctgcta
     5461 cagccccttc ttctataaat ccaatattta aattgtacaa ctttgcagga tttatagaca
     5521 ttttttctat taactgcatc atactaagat gatttttatg caccaaattt gttattccaa
     5581 gagctaaaga agtttcaagc ccaataattc cacttggagc ttttataaac tctctatctt
     5641 tttcttcttt actatgaggt gcatggtccg ttgctattat ttcaatagta ccatcattta
     5701 atccctctat aatcttatct ctgtcccatt tacttcttag aggtggattc atttttgcat
     5761 ttgttccaaa ctccaataca tcttcctcag tcaatgtaaa gtgatgtggt gaagcttctg
     5821 caactacatt tgcacctaag tattttgcaa atctaaccat atcaacagat acacctgaac
     5881 ttatatgttg tatatcaact ttagcaccag tctttagtgc caacatacaa tctctggcta
     5941 ccataacatc ttctgctaca cttgatgcac cttttaatcc tagttctgaa gctacctttc
     6001 ctgcatttac acctggattt ccaactaaag aagggtcttc ttcatgaaaa ctaagtggta
     6061 catccaactc ttttgcctta atcattgcct ccataattag attgctatcc attaaaggta
     6121 atccatcatc agtaaaccct ggtacaccag cttttttaag agcttccata tcaactaact
     6181 ctttaccttc aaaaccttta gttatagctg ctgcttgtaa aacatttata catgcatttt
     6241 ttgacttttc tttaacataa ttaaaagtat cttcattatc tacaatagga ttagtatttg
     6301 ccatacatat aacagttgta aatccacctc tagcagcaga tttagctcca ctttctatat
     6361 cttctttata tgtaaaccca gggtctctaa agtgtacatg tacatccact aaacctggag
     6421 caactacatt accactagca tctattattt tttcatattc atcactttca tcaaacttgc
     6481 ctattttata aattttattt tcttttataa ttaagtcaat attctcatct ctttgacttt
     6541 tagggtctat tactctaccg tttttaatta aaatcatatt atttctcctt tatattttta
     6601 atttatatag actcatataa tattatacat tattttacat acttaaatac aaaattacgt
     6661 ttgacatcaa tagccttttt aggacaaagt tcatggcaac agtaacatct tatacattta
     6721 cttaagtcta taatagcacc cttagtccct atctctatag tggatgcagg acaagcttcc
     6781 ttgcattttc cacattttat gcaatcttga aatcttacca caggctttgg tgttataagc
     6841 tttgaaatag gttcatttaa aaattttggt agctttacgg aaaacaatct taaatcctta
     6901 cttacatttg gaatcttgta attttttata attaaatctt ttatatcttc acctaaaatt
     6961 tcaatatctg aaaaatcttc ttgtatatat cctctcttaa tagcacctct aattgtagca
     7021 actctatttg gaagtaaact tataatctta catgcaacaa catctatagc atatggattt
     7081 tgtgatgcta ataaaactcc tatttctcga gtagtaccag ctgaaggtcc atcaccttcc
     7141 atacctacta taccatccat aattgtcaat gttggtttta tataatcaca aatatctaaa
     7201 agtacatttt cgcaaaacaa ctcttctgta ggaaatctat aatgtatctc tgcttttttc
     7261 aatccagcta tgcatccata taagtttttt gtaccaccag taaaagttgc cattatatga
     7321 gtctttaatt tacaaagatt tattatataa tctgaatcta ctattggttt aataatatcc
     7381 atatatttta gaacatgtgc tttttcacta tgtatcttaa catctgaaac atcataattt
     7441 aacttaatgc ccagttcatt tgctacttct tcaattccag atgccttata aatgctctta
     7501 agagaacttt gattatatgg accaccaggg ctatctgcta ttgtaatatc acaatttaac
     7561 tctaaaagtt tttcacatac aacttttaca accataggat gtgtagttgt agcttcttca
     7621 ggcttttttt tcattaataa atttggtttt ataagtactt tgcttccttt tttaacaaac
     7681 ttatctatac caccaatatc ctctaaattt ttttcaatag ataactttac ttcttcataa
     7741 ttgtaagttt tacaactcct aacagatacc gacttcatat tatctccatc cttctatcac
     7801 aacagtttat ttgtataatt taaaaaagga gaaactaaag tttctccttt taatctatgt
     7861 tttaattgtt ttaattatta ttttccaaaa tgtctgttta ataatcctaa gaatgcttta
     7921 ccatgtctag cttcgtcttt gcacatttca tgtactgtat catgtatagc atctaatcct
     7981 aattctttag ctctcttagc taattttaat tttccatcag ttgcaccata ctcagcatca
     8041 actctaactc ttaagttttc ttttgtatct gcaactacaa cttctccaag aagttctgca
     8101 aatttagcag catgttcagc ctcttcaaaa gctattctct tatacgcttc agctacttct
     8161 ggataacctt ctctatcagc ttgtctactc attgctaaat acattcctac ttctgtacac
     8221 tcaccagtaa agttagctct taatccttcg attatttctg catctacacc ttgagctact
     8281 cctattctat gttcatcagc ccaaaccatt tcgcctttca tttcttcaaa tttatcagct
     8341 ccaactttac atactggaca ttgtgctggt gcagcatctc cttcatgtat atatccacat
     8401 actgtacaaa caaatttttt cataattaat ttcctcccaa ttttatataa ttaaattttt
     8461 atttttaagt ttaataactt gctacaagaa tatatatacc ccagacttta tagcttaaac
     8521 actaaaaata aaatttattt cctaattaat tttatcactt tgaaacacca gtatcaacca
     8581 agttttgatg tcaaatttct acataataca taatttattt gattaattag gaaatagttg
     8641 tcatattaaa tactttctaa tgaagactcc tctgaatctt taagtttcac ttttttgctc
     8701 tttaaaaaag ataatttagt ttctgcagtt atgattgcaa caaatatcaa gatactacca
     8761 ataaccaatt ttgctgtaat aagctccttt aaaattatta tagaaaatat tgtaccaaat
     8821 acagcctctg tggataatat tatagctgtt ctagttccat caactctttt ttgacatata
     8881 gtctgaaata aaaatccaat tgtagtacta aatacagcta gatacatagt tcccatgtaa
     8941 ccagataatt cagcttccat ctttaattga cctgcaaatg tttgaactac aacagacatt
     9001 aaaaacgcta ctgtaaattg aatagctgtc aatgccattg ggttgttatc ttttgcaaac
     9061 tcacttgtaa agaatatatg aaatgcaaat ccgaatgaac atataagtgt tagaaaatct
     9121 ccaaaattaa tagagaaatc agcttctaaa gaaagtatcc caattcctat aagagctacc
     9181 aaactactta ttattcctat tttatcaagt tttcttctat aaagtataaa tcctatgaac
     9241 ggaactatta caacattagc tgctgttata aaagcatttt tagatggtgt agtgtacata
     9301 agtccaatag tttgtactgt gaatgctaga aataaaaata ttcctaaaat ccctcctgct
     9361 tttaataatt ttttacccat attggcacga atctgtttaa aaaacagtaa attcataatt
     9421 atagctgcca agaaaaatct taaagttatg atttgtagag gtgtaagccc accgtctaat
     9481 gcaaattggg ttgctacaaa gccacttccc catataattg ctataaatat tagtcctatt
     9541 tctccaaagt attttttcaa taaagccccc tcctttttat ttttttgcaa aattataaac
     9601 cccctaaaca gcaagagagt ttttctataa gcattactat aaattaatat agtaaatttt
     9661 ttcagtattt taaatctaaa tttacataca ttttgagctc aaatttatat
//
LOCUS       DACXMZ010000152         9671 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.153, whole genome shotgun sequence.
ACCESSION   DACXMZ010000152 DACXMZ010000000
VERSION     DACXMZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.153"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            416..2494
                     /locus_tag="JD016_13845"
     CDS             416..2494
                     /locus_tag="JD016_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAU5000716.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            2494..3273
                     /locus_tag="JD016_13850"
     CDS             2494..3273
                     /locus_tag="JD016_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAU5000717.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            3266..4075
                     /locus_tag="JD016_13855"
     CDS             3266..4075
                     /locus_tag="JD016_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAU5000718.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            4095..4577
                     /locus_tag="JD016_13860"
     CDS             4095..4577
                     /locus_tag="JD016_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU5000719.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            4598..5002
                     /locus_tag="JD016_13865"
     CDS             4598..5002
                     /locus_tag="JD016_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAU5000720.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(5158..6318)
                     /locus_tag="JD016_13870"
     CDS             complement(5158..6318)
                     /locus_tag="JD016_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HAU5000721.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            6532..8076
                     /gene="xylB"
                     /locus_tag="JD016_13875"
     CDS             6532..8076
                     /gene="xylB"
                     /locus_tag="JD016_13875"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAU5000722.1"
                     /translation="MEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGWAE
                     QDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIWCD
                     QRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLPKD
                     YIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITGYV
                     TRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPKID
                     KEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIINNQ
                     VSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYSLK
                     DCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAILAL
                     VGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDEVV
                     NAKIND"
     gene            8172..9509
                     /gene="xylA"
                     /locus_tag="JD016_13880"
     CDS             8172..9509
                     /gene="xylA"
                     /locus_tag="JD016_13880"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAU5000723.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
BASE COUNT         3676 a         1120 c         1801 g         3074 t
ORIGIN      
        1 aataaattat ttataattat aaaatccatt gttttgaaca atatctaata tataattatc
       61 tgtatttata aggtcactat acatattttc aagtccgata ttatctacat aataggcaaa
      121 ctttattcct gtatgtgttt taaaaaaact agatgtatat aggtatcatc acagatatta
      181 aaagtataaa taaaagatat taagaacgac tttccagcaa catattagaa aattttacta
      241 aaatccggaa agcgattgac aaacaaagtc aaataaagta tattattggt aagttagttt
      301 aatgtacaaa tgaattatat ataaaaccta atttataaat aacgatacta aacgacatta
      361 tttaacttaa gctacacaaa gatagaatat tataccattg atttaggagg tagccgtgat
      421 aaaaatatct gaagactata ttgaaaaaag atttgacaat gaactattgc gaatagaagc
      481 atggggaaaa aactctttac gtattaggtc gtttgtagac caaaattttg ttgatgaaaa
      541 ctatgctctt aacgaaaaac ctaagttaaa taaagatgat attacaatta ataaaaatga
      601 agatggaagt gcaatcatta aaaatggaaa gataaaagct gtcttagacc atagagatag
      661 aattactttt tataatgata aaaatgaaat attattgaaa gaatatattc gattaagagc
      721 tgtaaaacat gatgatggtg gagaagatgt agggacaatt gagattacca aagactttaa
      781 ctctactcta aagttaaaat caagagaata tcgaccaagc tatagtggag aatttgaagt
      841 aactactaga tttgaatctg agccttctga aaagattttt ggaatgggac aatatcaaca
      901 taaattttta gatttaaaga atactgtttt ggagttagcc caaagaaatt ctcaaatatc
      961 agttcctttt tatatttcta gtctaggtta tggactttta tggaataatc cgggaattgg
     1021 aaaagtatct tttgctaaaa atatgacaga atggaagatg ttttctacaa attttataga
     1081 ttattggatt acttgtggag aaagcccaaa agagttaaat aaaaattact ctcaagttac
     1141 tggaactgtt cctatgatgc ctgaaaacct tttaggacta tggcaaagta aactaagata
     1201 tagaacttca gaagaagttt tagatgttgt gaaggaatat tctaaaagag gaattaagct
     1261 ttcatcaatt gcaattgact attttcattg gcctaagcaa ggtgaatata aatttgactt
     1321 agattattgg aaaaatccta aagaattagt taaaaaattg aaagaagagt attcagtaga
     1381 accaatagta tctgtatggc ctacagttca aagtgatgca gaaaactaca atgattattt
     1441 agaaaatggc tatttagtca atgttaatcg tggtgttaga atgacaatgc aaattcaagg
     1501 aaatacagtc tttgtagata tgacaaatga aaatgcaaga gaatatgttt gggatagaat
     1561 agataaaaat tataaacaac ttgggattga ctattattgg ttagatgtag ctgaacctgg
     1621 ctattctgtg tatgattttg ataactatag atataaaaaa ggaaatgttt taagttgtgg
     1681 taatatttat cctattgatt atttaaaaat gatttatgat ggtttacata atgacataga
     1741 gtctgttgta actcttgtaa gaggagcttg ggcaggagct caaaagtatg gagctttagt
     1801 atggtctggt gacattgatt ctagttttga agcttttaat aatcaagtaa atacagggtt
     1861 aaatatggga ctggcaggta taccatggtg gactactgat attggaggtt tccatggagg
     1921 aaatcctaaa gacccagaat ttagagaact tatggtaaga tggttccaat atgctacttt
     1981 ttcaccaatt ttgagaatgc atggagatag attgcctcat agtaaaccat tatcaaataa
     2041 aggtggaggc tcaatggtaa ctggagctcc taatgaaata tggtcttatg gtgaagaagt
     2101 tgaagtaatt cttacaaaat tcataaaaat tagagagagc ttaaaaactt atttaaaaaa
     2161 actgatgaaa gaagcgcatg aagatggaac accagttatg agaactttat tttatgaatt
     2221 tcctgaagat gataaaactt gggaggtgga taatacatat atgcttggag atgaaatttt
     2281 ggtagctcca ataatgaatt ataaagatag aagtagaaaa gtatatttac caaaaggaca
     2341 tacatgggaa aatatattta gtggagttag ttatgaaggt gggaaaactt atgaagttga
     2401 atgcccactg gaagaaattc ctattttcct aaagcaagat tccagttata attttaaaga
     2461 actaaaaaat attttgggag aggtgaaatc ataatggagc cacaattatg gcaattatta
     2521 ttaatcgtac tatatggatt ttttataaac tatgaaaaaa attcaacaat gtttggaaca
     2581 tatcaaccag taacagcagg ttttattact ggtcttatct taggagatat aaatacaggg
     2641 ttatatattg gaggtacatt acagctatta tcattaggta ttagtaactt tggtggagca
     2701 tcaatacctg attatcaaac agcaagtata gtagccacat ttataactat tacgactaag
     2761 caagaagcat ctgtaggaat ctctataggt attccagttg ctttattgat ggtacaatta
     2821 gatgttctta gaaatactat tggtatatgg ctagttcata aagcagaaga tggagctaaa
     2881 aaaggaaatt ataaaaatat aacctatatg caaatgttag gagtactatt aacagcagct
     2941 acaacaggta ttccagttgc tctatctgta atatttggac cttctttaat taataccatt
     3001 ttgaaatata cacctgaatg gttaacaggt ggtttaacag tagctggagg actacttcca
     3061 gcagtaggga taggattatt acttagatat cttcctgcta aggagtattt tagttactta
     3121 gtaattggtt ttgttttagc agtatatatg aaagtaccat tattaggtgt agctttaatt
     3181 ggtggagcaa ttgcacttat aatttacaaa aagaatttgg aaaatcaaga acaacaatat
     3241 actgtagtag gaggtatgga cgaagatgag tgataaggta tcattaaata aatcaaatga
     3301 aaaggtgaaa aaaagtgtct tatggcgctg gttttttact agttctgttt cttcaaatta
     3361 tgagaagatg caggccttgg catactgtta tgcagtacta ccttttttaa aagtagtata
     3421 caaagataat ccagaaaagt tacaaagtgc tgtattaaat catttacaat tttttaacac
     3481 taatccttgg gttgcccctt atattttggg tattaatgta gcaatggagg agttatcaag
     3541 tgaagagact gaagaggctg ttgcttcaat taaaacaggc ttgatgggac cagtagcagg
     3601 cttaggagat agtttatttg tcgttattcc gtggacaatt tttggagcta tagcagctaa
     3661 tatggcgata gatggaagtc catttggaat cttactgtgg attgtagtga gtgtagcatt
     3721 gaaattatta agtataccac tatttaatgc tggttataca tcaggtacta agctaatatc
     3781 ttcaattgaa aaaagtttaa agattttaac tgagagtaca tctattttag gtttaatggt
     3841 tgtaggtgca ttaatacctt cagttgtaaa agctaatgtt gctttgaact ttcaacaagg
     3901 tgattttaca atgaaaggtc aagaaatatt agaccaaatt atgcctgggt tagttcctgc
     3961 aattctagtt gtgattgttt attgggctct taaaaagaat ataaaaccta tatacttaat
     4021 tttaggagta atggttgttt caataatttt atatgcatta ggtattttaa aataaggaaa
     4081 ggatgatatt agatatggaa atgaaaggta ttaaaaatat tcgtatagat gaccgtttaa
     4141 tccatggtca agttgcaact atgtggagta ataaattggg tgttacaaga ttaatggtag
     4201 taaatgatgc agtagctaac aattctgttc aaaaacaagt tttaagaatg gctacacctg
     4261 caggtatagc gtcatctata attacagaag agactgctat aaaaaatatt actgcaggaa
     4321 agtatgaagg acaaaatgta ttattaattg ttaagtctcc tgtggacctt attccatttg
     4381 tagaggcagg atttaaaata cataatataa atgtaggaaa tatgtcaagt agaaaagata
     4441 caacagttct aagacctaat atcagtgtta ctgaagaaga gaaagaagca tttaaaagac
     4501 ttttagatga aggaattgaa attacaacta taatgacgcc tgatgataag aaaacttatc
     4561 ttgtagacat cttatagatt taaagaggtg tattaaattg aatagtatta ttttgatgag
     4621 tcatggcaat atggctgaag aagtcttaaa ttctgcaaaa atgataatag gggattctat
     4681 agattatcct actgtaaata tgaaacaaga tgatggaata gaaggaacaa tagaaaaatt
     4741 aaaaaaagct ttagaagtgt acaaggaaag ttcagaaata attataatgg cagatttact
     4801 aggtggaact ccatgcaatg ctgctttatt agtagccaat aatgacagtc gaattaaaat
     4861 tatttcaggg ctaaatttag gtatggttat tgaatcagct tttttagata caaaggaatt
     4921 ggcagcaaaa ttagagaaaa taggtaaaga taatatttct atggttgaac aaaatgcaag
     4981 tttattatct gatgatgagt aggaagatta tattaatatt taaaacaaaa tacttatgtt
     5041 aatacttaat tgatatacaa tttttaaaaa atatagctaa tcttgcataa actatataat
     5101 aaatctaatt ttagaatgta taatttaaag ggttatggct gtatatagtc ataaccctta
     5161 tatttcatta attaaagtta aattttgtat attcaaaaag ctcttgatat ttacggcaga
     5221 gccaccatat agagatgcct cagaccctaa tgatgaaatt tctatattta ttcctttgtt
     5281 aaaagagcta actaacatat cttttgtaat ctgtaatatc tcagggatat cactaattat
     5341 ttggctattt aaatatatta tttcaggagc ataagttgta atagcattat ttatagctat
     5401 tgttaagtaa ctacaaaact catgaataac ttttttggca ttctggttat cttcataata
     5461 tagttgcttt actatatcag aatctatttt aggaatattt tctaaagaag aaagctgttc
     5521 aaataccttt ttttctgaac aatactgttc taaacagcca cgatttccac aaggacatag
     5581 tttaccgtta ggcatgatga tagtgtgacc aatttctcca ctcataccat ttctgccact
     5641 atataattta ttatttatta taatgcctga accaaatcca ctgtgaatac tgagactaag
     5701 tagtgagtta tgtacagttg aaaaagtatt ctcagctaaa gctgttaagt ttgcttcatt
     5761 ttctatatga ataggaaagt catacttttt gctaaggatg ctatataagt caatctcatt
     5821 aagattgtaa tatggagtaa acaatacttt attttcacaa gtaatcccat gtatagccaa
     5881 agtaagacca attaccttat aaggagtgtc tattttggat atgttataac tatttataat
     5941 ttcatctatc aattgtataa cattgtcttt acttacttgt atatctgtta atttcttaga
     6001 atttataata gttccatcta agtaagatag agaagaaaat atatagtcgt atccaatgtc
     6061 catacttaaa gaaatccctg cacatttatt aaatactaat aatatgggtt ttcttccgcc
     6121 actatgagta ctatttccaa ttccgatttc atgaactaga gattcatcaa taagtttttt
     6181 ggtaatagcg gatatagttg ctttattcaa tcctatagta gaagctatac ttgccctaga
     6241 aataggacca ttttttataa tttgttcaag taccaatctt tcattcattt ctcgaatagt
     6301 gtatttatca gtaaccaatt tgatattcct ccttaaaata atattgtaat actttttaca
     6361 caaaaataaa aggttatttt gcattgacaa agataattaa atattttatt attagttcat
     6421 aagttagttt aatatactaa caaaaataaa gcaagtaaaa tatacctaaa atataaaaaa
     6481 attaggatag gaaaacgata gttatgaagt ggcattcaag gagggatgaa gttggagtat
     6541 gtattaggtg tggatattgg tacatcaggt acgaaaacag ttttatttga taaactagga
     6601 aatactataa aaagttgtac atatgaatat cctcttatac aagagaaatc aggttgggca
     6661 gagcaggatg caaatgattg gtggaaagct gtagtcgaat ctataagaga agtagttcaa
     6721 agttcaaata taagcagtga atgtataaaa ggaattggat tatcaggaca gatgcatggt
     6781 ctagttatgc tggacaatga aggtaaaaca ttgagaaact ctataatatg gtgtgaccaa
     6841 cgaacagtaa aagagtgtga ggagataact gatttagtag gtgaagaaag actgattgaa
     6901 ataactgcta atccagcact tacaggattt acagcatcaa agatattatg ggttagaaac
     6961 aatgaaccag atatatataa aaatactaat aaaatacttc ttccaaaaga ttatataaga
     7021 tacaaattga caggtgaata tgctacagag gtatcagatg caagtggtat gcagttgctc
     7081 gatataagaa aaagagattg gagcgatgaa gtactagaaa agttaaatat agataaaaat
     7141 cttttaggta aagtttatga atcacaagag attacaggtt atgtgactag agatgtagca
     7201 agtttgacag ggcttaaaga aggaacaata gtggtaggag gagctggaga ccaagcagca
     7261 ggagctatag gaaatggaat agttaaagat ggagtagttt catctactat aggtacatca
     7321 ggtgtagtat ttgcttatac aaaagagcca aaaattgata aagaaggtag agttcatact
     7381 ttctgtcatg caataccaaa tacatggcat gttatgggag ttactcaagg agctggcttg
     7441 tcattaaaat ggtttaaaga taatttctgt caaagtgaag tggaagtttc aaattcttta
     7501 ggtgaagatg tatatgagat tataaataat caagtaagta aagtaccaac aggatgtaat
     7561 ggattgcttt acttaccata tatgatggga gaaagaactc ctcatcttga cccttatgca
     7621 agaggtgtgt tctttggatt atctccaatc cattctaaaa aagaaattgc aaggtcaata
     7681 atggaaggtg tttcatatag tcttaaagac tgtatggata taatagagaa tcttaatata
     7741 gacgttaatg aagtaagagc ctcaggtgga ggtggaaaaa gtaaagtatg gagacagatg
     7801 caagcagata tgtttaatca agatgtatat actataaatt caagtgaagg accagcactt
     7861 ggagttgcaa ttcttgcatt agtaggagct ggaatatatg aaaatataca aaaagcatgt
     7921 gatgctataa taaaaacttc aactaaatta gaaccaataa gtgaaaatgt agatacatac
     7981 aatatatatc atagattgta taaaagaata tacaaatctt tgaaagatga ctttaagtta
     8041 ttagatgaag tagttaatgc aaaaataaat gattaaatta gcttaaaaag aaaaataaag
     8101 atagaaaata gttttaaatt aagaaaacat actacatata ttatatgtgt aaaatattag
     8161 gaggacatga aatgagtgaa atatttaaag gaataggaca gataaaattt gaaggagtaa
     8221 aatctgacaa tgaattagca tttagatact acaatcctga acaagtggta ggtaataaga
     8281 ctatgaaaga acacttaaga tttgcaatga gctattggca tactttatgt ggagagggta
     8341 atgacccatt tggagtaggc acagtagaga gaccttggaa taatataaca gacccaattg
     8401 agatagcaaa gattaaagtt gatgcaggat ttgaatttat gtctaagatg ggaattgagt
     8461 atttctgttt tcatgacaga gatatagcac cagaaggaag agatttagaa gagacaaata
     8521 agatattaga tgaaatagtt gagtatataa aagttaatat ggaaaagaca ggtataaagt
     8581 tattatgggg aactgccaat atgtttggaa atccaagatt tgttcatggt gcatctacaa
     8641 catgtaatgc agatgtatat gcatatgcag cagctcaggt taaaaaagct atggaaataa
     8701 ctaagtactt aggaggagaa aattttgtat tctggggtgg aagagaaggt tatgaaactt
     8761 tacttaacac taatacagaa cttgaaatgg ataactttgc aagattctta caaatggctg
     8821 tagactatgc taaggaaata ggattcactg gacaattctt aatagagcct aagccaaaag
     8881 agcctactaa acatcaatac gattttgata ctgctactgt tttaggattt ttaagaaagt
     8941 ataatctgga taaatacttc aaaatgaata tagaagcaaa ccatgcaaca cttgcaggac
     9001 atactttcca acatgaatta aatatagcaa gaataaacaa tgttttaggt agtatagatg
     9061 caaatcaagg agacctatta ttaggttggg atacagacca atttccaaca aatatatatg
     9121 atgcaacact tgctatgtat gaagtgttaa agcaaggtgg aatagcacca ggaggattta
     9181 actttgactc taaagtaaga agagcatctt ttgaggtaga agacttattc ctagcttata
     9241 ttgcaggaat ggatacattt gcaaagggat tattaatagc tcataaatta ttagaagatg
     9301 aggtatttga gaactttact aaagaaagat atgcaagttt tagtgaaggt ataggtaagg
     9361 atatagtaga aggtaaggtt ggatttaaag agttagaatc ttatgcactg caaatgccag
     9421 ttataaagaa taagtctgga agacaagaaa tgctagaagc tatacttaac agatatatat
     9481 atgaagtaga tacaatttct aataagtaat tgtgtagtaa ttaaacttgg atgtttgaat
     9541 aaaataacag gatatgttct tgtttttatt ctaaaacgga caaagataag tatttgtaaa
     9601 aattctcata atcatgataa attaaggata tagacttaag tataattagt ttatatcctt
     9661 aaaatttcta t
//
LOCUS       DACXMZ010000153         9618 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.154, whole genome shotgun sequence.
ACCESSION   DACXMZ010000153 DACXMZ010000000
VERSION     DACXMZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9618
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.154"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..321
                     /locus_tag="JD016_13885"
     CDS             <1..321
                     /locus_tag="JD016_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAU5000724.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKS
                     VYGK"
     gene            454..1824
                     /gene="hydA"
                     /locus_tag="JD016_13890"
     CDS             454..1824
                     /gene="hydA"
                     /locus_tag="JD016_13890"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HAU5000725.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLETKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            1842..3248
                     /locus_tag="JD016_13895"
     CDS             1842..3248
                     /locus_tag="JD016_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAU5000726.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSRILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            3320..3766
                     /locus_tag="JD016_13900"
     CDS             3320..3766
                     /locus_tag="JD016_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HAU5000727.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            3805..4599
                     /locus_tag="JD016_13905"
     CDS             3805..4599
                     /locus_tag="JD016_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAU5000728.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            4589..6880
                     /locus_tag="JD016_13910"
     CDS             4589..6880
                     /locus_tag="JD016_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAU5000729.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGSDKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEQFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            6881..8194
                     /locus_tag="JD016_13915"
     CDS             6881..8194
                     /locus_tag="JD016_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAU5000730.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            8230..9315
                     /locus_tag="JD016_13920"
     CDS             8230..9315
                     /locus_tag="JD016_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAU5000731.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
BASE COUNT         3782 a         1034 c         1967 g         2835 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggttatatg acgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctatgcaggt
      181 gaagctggaa taaaaactat aggatttggt ccttctaagg aaaatcttgc tcacacaata
      241 gatgagtata tagagcaaga acagttattt ataggaacag aaggttacta tggaatatta
      301 aaatctgtat acggaaaata atatttagtt cacaataaat tatttaaata tagtctagag
      361 gtgctgaata tagcaccttt agaaaataca attttaaata aagttttaca atacttagaa
      421 aatactttaa agcttaaaag ttaggaggta tatatgggaa caatactaag aggtggaact
      481 ataattacct ctgataagac ttatataagt gatttaagga tagaagatga aaagatagtt
      541 gaaataggaa ataacttaga aataaatggt gataaggtta ttgatgctac aggtaaaatt
      601 gtaattcctg gtggaataga cacacatact cattttgata tgaatgcagg ttctattaca
      661 actgcagata attttgaaac aggaacaaga gcagctatag ctggaggaac tactacaata
      721 ctagattttg cagaagccaa tgagggggaa aacctactgc aaggtgtaga agcatatcat
      781 aaaaaagcaa gtggaaattg ttattgtgat tatggttttc atatgacaat tacatgttta
      841 aataaagata catttaatca tatggaaaaa cttatttcgg atgggatagt atcttttaag
      901 atgtatatgg catatgatgg catgaaagta gatgatggaa caatatacag ggttttaaag
      961 aaagcaaggg atttaggttg tatagttgga tttcattgtg aaaatgggga tttactagat
     1021 gtattgatag aagaaaatat acaaaatggt aatttagaga caaaatatca tccacttaca
     1081 agacctaaca tagttgagaa agagtctgtt tcaagacttg ctgatataac taaattatct
     1141 aattcaaaat cctatgtagt ccacttaagt tgtagggagt cacttgaaac tgtaaaaatg
     1201 gctagagaaa aaaacataga tatgatagta gaaacatgcc cacagtatct attattagaa
     1261 gacaatttat ataataagga taaatttgag ggtgctaagt atgtaatgtc tcctccttta
     1321 agaaaaaaag aagacataaa ttacctttgg gaaggattgt ctagtggaga tattcaaaca
     1381 gttggcacag accactgttc atttaatttt aaggggcaaa aagacttggg aattgatgat
     1441 ttcagtaaga ttccaaatgg agcacctggt gttgaacata ggttagcact tttatataca
     1501 tatggggtct tagaaaatag aataagtgta aataagtttg tggaagttac ttctacgaat
     1561 gctgcaaaaa tatttggaat gtatcctaaa aaaggtgaga tagctgtagg aagtgatgca
     1621 gatatagtca ttttaaatat caataaagaa gaaacaatta gttataaaac tcaaaaacaa
     1681 aatgtagatt atactccata tgaaggattt aaggttaaat gtaaagttga ggatgtattt
     1741 ttgagaggaa atcatgtagt acaaagttgt aatgttaaag aacatccaac aggtcaatat
     1801 attaaaagaa aaattactaa ataatgcggg gggaaataag aatgatggat atacttatta
     1861 agaatgccat aatagtaact gtgaataaag agagagaagt tatttttgat ggggcattag
     1921 tagtaaaaga taacaagatt gctgatattg gaaattctaa agagatagaa agtaaatata
     1981 ctgatgttaa aaaaattata gatgctaaag gtaaagtttt attcccaggt tttataaata
     2041 cacataatca tttattccaa actttattaa aaggattggg agatgatatg gtacttaaag
     2101 actggttaga aactatgaca tttccagcag ctaactattt agagccaaaa gatacttatg
     2161 atgctgcaat gcttggatgt attgaaggac ttagaagtgg tataacaaca atggttgact
     2221 acatgtatcc tcatagtaag ccaggtcttt gtgatggaat aatagatgct tataaagaat
     2281 taggaataag aggaatactt ggaagaggat gtatgaatac aggagctcaa tttggagttc
     2341 atcctggaat aatgcaagat gtagaaacgg ttgaaaaaga tgtaagaaga ttgtttgaaa
     2401 aacaccataa tacagaaaat ggaagaatca aaataggagt agctcctgca gcaatatggt
     2461 caaattctca agaaatgctt gaaatgctat ggagagtagt aaaagaatat gatgatgctt
     2521 tatttacagt acatatatca gagactcctt ttgatagaga agcagcaaaa gaattgcatg
     2581 ggcaatatga catagatgtt ttagaaaaat taggaatact tggaccaaat gtgcttatgg
     2641 tacattgtgt gtatctaact gaaaaagata tggaacttac taaaaaatac gatatgaaag
     2701 tgtctcataa tacagcaagt aatatgtact tatcttcagg tgttgcacca gtgccagaaa
     2761 tgcttaaaaa aggaataaca gttagtttag gagtagatgg agccgctagt aataactctc
     2821 aagatatgct tgaacttatg aaattaacag cacttcaaca taaagttaat aagtgtgacc
     2881 cattagcaat gtcagcagaa aaagttcttg agttggcaac tatagatgga gcaagagcta
     2941 ttggaatgga agatgagata ggttcattgg agataggtaa aaaagcagac ttattaatat
     3001 ttaatccaat gctttcacca aaagcgatac ctatgcataa tccggtatct acattagtat
     3061 attcttctag tatgaaaaat atagagagtg ttatagtaga tggaaatata ataatggaag
     3121 attcaagaat attgacagct aatgaagaga aagcactaaa agatgctcaa gatacagctg
     3181 aaagactttg tgtaagagga actataaaaa atagaatgga aggtcataaa tggaatagcc
     3241 tttattaata aatgtaattt attcataaaa aagtatagta aaactttagc ttaaaaatat
     3301 gagttttgag gtgatttatt tgattatcaa atgtaccata aataataaag aaaaagaatt
     3361 aagtataaga ccagatgatt ttttagcaga tacattaaga gaaaatggat atgtaagtgt
     3421 taaaaaaggg tgtgatacag gctcatgtgg gttatgtaca gtgatggtaa atgacaaggc
     3481 tgtgctttca tgtaattatc ttactgctag agtagaaggt cagcatatta caacacttga
     3541 gggtgtcttg gatgaagcaa agaagtttgg agattttctt gcagacgaag gggctgacca
     3601 gtgtggattt tgtgctcctg gatttataat gatggttttg gcaatgaaaa gagagcttca
     3661 aaatccaaca gaagaagaag ttatagatta tgtaaatggt aacctttgta gatgtacagg
     3721 atataaaggt caattaagag caatacataa atatttaaac acttagactt ttaagtgaaa
     3781 attaagttga tatggggaga gagtatgata actataaagg aatatgtagt tccaaaaagt
     3841 ttagatgaag cttatgaact gctaatatca agaaaaaaca acataatact tggaggatgt
     3901 ggatttttaa aactaggtag taaaaatatt ggttcagcaa tagatttaaa agacttagct
     3961 ttagattata taaatgaaac tgatgatagc atattaattg gagcagatac aagtttaaga
     4021 actttagaat taaataaggt tattaaaaat tactgtaatg gggtaatttc aaacgctgtt
     4081 tctaatatag taggagttca atttagaagt ggagctagag taggagctag cgtatttgct
     4141 aagtatggat tttcagattt aataccttca ttactagtgg tagatgcaaa ggttaagtta
     4201 tataaaaaag gggttatgaa cttagctgat tttttagaaa atgagcttga aaaagatata
     4261 ttaatagaag ttatattacc taaaaaagat gctataggtg tatttgattc tataagaaaa
     4321 tgtacaggtg attttgcagt attaaatgga gcaatgttaa aagaaggaag tatttataaa
     4381 attgcgatag gcgctagacc aagaagagca aaaattgcct ataaagcaag tgaaatatta
     4441 agtttagaaa atgatataga aaaagctggt gaagtatcaa gtgaagaact ttcatttgga
     4501 agtaatataa gaggaagtaa agaatacaga aaagatatgg caaaagcctt agtagtgaga
     4561 atgtataata gtattggagg tgagtgtgat ggtaaataaa gaggtaagaa aaatagatag
     4621 tgaagccatt gttactggaa aaccaatata tacagaagat ttaatttttc ataaagatgt
     4681 tttaacaatt aagcttctta gaagtccaca tgcttttgcg aagataaaaa acatagatat
     4741 aaaaaatgca ttaaaggtaa aaggtgtagt aaacatattc acttacaaag atgttccaaa
     4801 ttatagatat tcaatggttg gagagtcata tccagaagca tcacctcatg accaattaat
     4861 acttgaagac gtagttagat atgtaggaga tgaagttgct ataatagccg ctgaagatga
     4921 aagggctgct attaaagcta tgaaacttat aaaggttgaa tatgaagtaa tgactcctat
     4981 attggatgca agagaatcgg aaggaaataa aattttaatc catgaagaag atgatttgtt
     5041 ttgtccattt gattttggat tggatgctac aaaaaatatt gtatcgagag aaaaaatggg
     5101 taaaggggat gtagaaaaag agtttaaaga aagtgatgta atagtagaac attcttatat
     5161 gactcaatca caagcccatt gtatgatgga aactcataga gcttatacgt atatagatac
     5221 gaatggcaga ttagtagtaa cttctgcaaa ccaatcagct tatcatatga gaagacaggt
     5281 ttctaaagca ctgggaatac ctctttcaaa agtgagagta ataaaaccaa gaataggtgg
     5341 aggttttggt ggaaaaaaca tagctgtaac agagatatat gcatcatttg taacttggat
     5401 gacaaaaaga ccagcaaaac taatttatac tagagaagaa acttttgcaa tgactaatac
     5461 tagacatcag atgttttttg atataaaaat tgggtcagat aaagaaggaa acataaaagc
     5521 tatagatatg aaagcattga ataatacagg cgcttatggt gataatggac cttcagtttg
     5581 ctctgaatca ggtcataatg tacttccaac atataataat gtacctgcaa taaaatttga
     5641 tgggagaact gtatatacta atttagttcc aggaggagca ttaagaggat atggagcaac
     5701 acaaggtaca tttgcacttg attgtgcaat tgatgaatta gcgcataaac ttaatatgga
     5761 cccaacagaa ataaagttaa agaatattat aaaaaaacaa actgaaggcg gaataatgaa
     5821 ttttccaata agaagttgtg cacttgatga atgtataatt agaggtaaaa aacttattgg
     5881 atgggatgaa aagtaccctg ttagggatat tggcaacaat aaggtgaggg ctgttggaat
     5941 gtctgtggga actcatggtt ctggtatagc caacatagac atggcaatag taaatatgag
     6001 gatggatgaa gatggaactt ataagttgtt ttctgggtct tcagacttag gaactggttc
     6061 tgacacaata cttgcacaaa tagctgcaaa agcattgaat acatctattg atagaatttc
     6121 cgtttataca ggagatacag atatgtgtcc atatgatact ggtgcatacg cttcttgtac
     6181 tacttatgta actggaaatg ctacaataaa atctgctgaa agtttaaaac aaaagatatt
     6241 aaaagtagca cagaagaaac tcgatttacc aatagatgat ttaatactat atgaagacag
     6301 agtttgttat agtgaagatg tagagcagtt tgttttgtta agtgaattag gaaaagaatc
     6361 tgttggtggt ggaaatcaag aagttttata ttcatctgaa tcttatggta tacaagagag
     6421 tgctaaacca ttcttagcag gatttgctga aatagagtta gataaaacaa caggggagtt
     6481 taaagtaata aattatgctt gttctacaga ttgtggtaca gttatgaatc ctgcacttgc
     6541 tagaatacaa gttgaaggtg gtgtaactca aggtataggg cttgcaatgt atgaagaccc
     6601 tagacatggt tctgatggaa agcttcaaac ttctaatcta ttacaataca aagttccaac
     6661 aaaaaaagat ataggaaaca taattgttga ttttgtagat aattatgagc caacaggtcc
     6721 atatggagct aaatctttag gagaaatagt tattcataca cctgcaccag caatagcaaa
     6781 tgcaatatac aatgccacag gagttcgtat aagagaactt cctataactt ctgagaaagt
     6841 ttatatggcc atgaaaaaat taaatcaggg aggtaaataa atgattgcta ttaaaagtaa
     6901 atacttaata tcttctgctg atgaaatata tacaaattct gctgttgtag tagaaaatga
     6961 cattataaaa gatatattgc caaatgaaga agttgaaata aaatataaaa atattgaaga
     7021 aataatagat aaatcagatg ctataataat gccaggattc gtcaatggtc atatgcatca
     7081 atatggagtt ctttctaggg gaataccggc aaatgtacat tttaccgact ttgaaggttt
     7141 cttaaatgac tattggtggc catttataga aaatagaatt ggattgaaag aagtgaaggc
     7201 aacaactaaa gcatcagcaa tagaacttat agaatctggt gtggtagctt tttgtgatac
     7261 cctagaagct ccaaatacag aagaaggaac tttaattgag caaggaaaaa tactagaaga
     7321 aataggcatg aaagcgattt tatccttaga aagttgtgaa agaataagct ttgaaaatgg
     7381 acttagatgt ttagatgaaa attctaattt aataaagtgg agtagagaaa atagtaaatt
     7441 aataaatgga ataatgtgta cacatacaag cttcacatgt tctgatagat ttattaagaa
     7501 agctaaagaa gatgcaaaaa aattaaatgc accaatgcaa tttcatttat gtgagagtat
     7561 atatgagcca aactatgctg aaaaacattt tggcaaaaaa gcagttgact actataatga
     7621 tttagatata ttggatgaaa ctgtattagc atctcagtgt gtaaaagtaa atgatgaaga
     7681 aatagatata ttaaaagaaa aaggtgtaaa agtggttcat atgcctctta gcaactgtga
     7741 agtaggtgga ggtttttctc cagtaccaaa aatgattaaa aaaggcataa aagtggcact
     7801 gggtactgat ggatatataa atgatttttt tgaagttatg agaggtgcat ttttaatgca
     7861 taaatcagtt gaagaagatg catcagtaat gccagcaaat ttagtattta gaatggcaac
     7921 agagcatggt gcttatgtat taggacttca aaattctggt aaaatagcag ttggtaataa
     7981 ggctgatata atagttatgg aagatgaatt taaaactcct gtgactcttg ataatatatt
     8041 tgaccagata gtagttcagg gtaaaaaaga atttatatca aatgtataca tagatggtag
     8101 acatatttta aaagaaaaac agttagttga tttagataaa aaagctattg ttaaagaaat
     8161 gaaagaagtt gcttgtgaat tttggaaatt ttaacgaata taaatatata tgaaaagggg
     8221 aagcagataa tgtataattt tttaggtaaa gaattaaaat caccacttat aataggttca
     8281 ggaccattaa catatagtgc agctggatgc aagatattaa gtgatgcagg agcaggggca
     8341 gttgtgacta agactataag aaaagaaaga gctataaatc cagcaccaca tatggttaga
     8401 aatacagcta atgctctttt aaataatgaa aaatggacag attttgagcc agagcaatgg
     8461 atagattttg aaataccaca aatgaaaaga gatgggactg tctgtatagc ttcaataggt
     8521 catacaattg aagaaagtag cgaattagta gagaaagttg caaatgcagg agctgacttt
     8581 attgaattag tatcttatga ctatagagat ttgataccaa tgcttaaaga tgcaaaggaa
     8641 agagtaaata tacctgtaat agttaaactt cctcctatga tagatgagat aggcgatttt
     8701 gctaaaaaat tagaagaagc aggagcagat gcaataactg cttgtgattc tgtaggacca
     8761 gcatttagaa tagacataga aacagggcaa cctttattag gtggaaatgg aataggatat
     8821 ttaagtggag aaactatcaa gccaataact ttacagagaa tatatgaaat aaaaaaacaa
     8881 gttaatatac caataattgg cttaggtgga tgtgtaagtg gagatgatgc cttagaaatg
     8941 attatggcag gtgctgactt tgttggtata tgttcagtag taattttaaa aggcgcacaa
     9001 gtaatatcta aaatacatga tgatttaaaa tctaatctta atagattagg atataatact
     9061 atagaaaatg cttgtggtat agtacataat catatgggag atgttaatga aagacttagc
     9121 tttgagttta cttatgatga agaaaaatgt actaaatgta agatgtgcga gagagtttgt
     9181 gcatatcaag cgagaaaatt gaatgataag atggaattga atgaagaaga gtgtagatat
     9241 tgtggacttt gtatatcagt ttgtaaacca aaagcacttg gaagaaaaat ttcatgttca
     9301 gagctgaatg tatagattat tttataaaaa acaaagtttt aaataaaaat aagaaaggct
     9361 atctcaaaga gataagcctt ttttgttgtt gaccttattt ttgtttattt gtagttattt
     9421 tagatattaa ttagttttga agtaataaag tgaatcaatt ttgtttaatt aaaattttaa
     9481 taattctgat atatatgtta acattgtata gcatttgtgt aaaatgttat aataattaat
     9541 agggatggtg atgcaagtgc aagagaagat tttagagtta ttatatacta taaatgaaga
     9601 tgtagttagc ataaaaaa
//
LOCUS       DACXMZ010000154         9567 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.155, whole genome shotgun sequence.
ACCESSION   DACXMZ010000154 DACXMZ010000000
VERSION     DACXMZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.155"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            34..1662
                     /gene="aspD"
                     /locus_tag="JD016_13925"
     CDS             34..1662
                     /gene="aspD"
                     /locus_tag="JD016_13925"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HAU5000732.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1783..2778
                     /locus_tag="JD016_13930"
     CDS             1783..2778
                     /locus_tag="JD016_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAU5000733.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(2956..3338)
                     /locus_tag="JD016_13935"
                     /pseudo
     CDS             complement(2956..3338)
                     /locus_tag="JD016_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089030.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3564..3661
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3803..4158
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3803..3831
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(4444..4602)
                     /locus_tag="JD016_13940"
     CDS             complement(4444..4602)
                     /locus_tag="JD016_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000734.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(4961..6331)
                     /locus_tag="JD016_13945"
     CDS             complement(4961..6331)
                     /locus_tag="JD016_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HAU5000735.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6615..6713
                     /locus_tag="JD016_13950"
                     /pseudo
     CDS             6615..6713
                     /locus_tag="JD016_13950"
                     /inference="COORDINATES: protein motif:HMM:PF14657.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            6732..7153
                     /locus_tag="JD016_13955"
                     /pseudo
     CDS             6732..7153
                     /locus_tag="JD016_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7384..7656
                     /locus_tag="JD016_13960"
     CDS             7384..7656
                     /locus_tag="JD016_13960"
                     /inference="COORDINATES: protein motif:HMM:PF00589.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAU5000736.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(8019..8099)
                     /locus_tag="JD016_13965"
                     /pseudo
     CDS             complement(8019..8099)
                     /locus_tag="JD016_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8139..8807)
                     /locus_tag="JD016_13970"
     CDS             complement(8139..8807)
                     /locus_tag="JD016_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAU5000737.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
BASE COUNT         3434 a         1436 c         1292 g         3405 t
ORIGIN      
        1 atgtgaaact tattacataa ggaggaattt aacatgaaaa atcatactta taaagaaatc
       61 aaaaacatat atggaaaaat aagccctttt gaatttaagg acaaattaat agatattgca
      121 aaatacactg caaaagaaaa taacagagaa ttacttgatg caggtcgtgg aaatccaaac
      181 tggacttgtt caactgctag agaagccttt tttacttttg gtcatttcgc tattactgaa
      241 actcgttcta attgggattt aggtcatttg gctggaatgc ctcaaaaaaa gggtattaaa
      301 gaaagatttt ttaaatttat aaatgaaaat atagatatgc ctggagcata tttagctaga
      361 gatattataa actttggtat aaatgaactt ggatttgatg gagatgaatt tgttcatgaa
      421 ctagctgatg gaattattgg tgataactat ccacttccag atagaatgtt acctcatatg
      481 gaaaaaatag tacacgatta cctagttcaa gaaatgaaat atgatatatc aggtaaatat
      541 ggtgatgtag aaatttttgc tgttgaaggt gctacagctg ctatgtgcta tatatttgat
      601 tcattaatgg caaatgaact tctaaaaaaa ggagatacaa tagccttaat gactccaata
      661 tttacacctt atcttgaaat acctaacctt cctcgttacg actttaaagt ggtaaatata
      721 aatgctaatg aagttgatga aaaaggtgct cacacatggc aatatacaaa aaaagaatta
      781 gagaaacttc gtgacaaatc tattaaagct ttattcgttg ttaatcctaa taatcctgct
      841 tctatagcta tggatgaaac atcttgtaat aatctaatag atgtaattga aaattataat
      901 aaagatttaa tgataatatc agatgatgta tatggaactt ttgtagaaga tttttcatcc
      961 ttaatgtcaa aactacctta caacactgtt ggagtatatt cttattcaaa atattttgga
     1021 gttacaggat ggagacttgg tacatttgcc cttcataaga aaaatgtttt tgataagaaa
     1081 ataaatgatt taactggaga attaaaaaaa tcagtagata agcgttacag tgatatgagt
     1141 cttaaccctt ctagcctttc atttatggaa agagttgttg ctgatagcag acttgttgct
     1201 ctcaaccata ctgctggtct ttctactcca cagcaagtac aaatggcgtt tttctctgct
     1261 tttgcattga ttgacaaagt agatgcctac aaaaaactca atatgagtat ctgccataga
     1321 cgtcaaaaac tattatttga agctttagag cttccaatta atgaaaacaa aaacaatgct
     1381 gcatattaca ctcaatttga tattgaagaa tgggctaaat taaactacgg agaagactta
     1441 tttaaattta taagtaaaac tgcatctcca gtagatgttc tttataaatt agctaatgac
     1501 tattcagtag ttttacttag tggaaatgga ttctatggtc ctgaatggtc aattagaata
     1561 tctctagcaa atctatatga tgaagcttat actaagatag gaaaagccat aagagagata
     1621 ttaaatggtt atatcgttga atggcaaaaa attaagaaat aattatatta cactgaaata
     1681 tctaaattat atgaataaaa gagtttatgt agaccttaac tacttttttt gatataatcc
     1741 tatcggaatt ataaatttta tttatgaaag gcagagaata gtatgagtat taagaaaaaa
     1801 gttgccatag tttttactgg tggaactata tctatgacag tagatgataa agtcggagca
     1861 gctataccaa ctttatcagg tgaacaaata ctatcaatgg ttaccaacat tgataaagtt
     1921 gcagatgttg aagtttttaa ttttgatgaa attccaggac ctcacataac accttataga
     1981 atgatggaat tgaaaaatta tgtaaatgat ttattagcaa gaaaagatat tacaggtgtt
     2041 gtaataacac atggtacaga tagtttagag gaaacagctt attttctaga ccttacaatt
     2101 gacaacatca aacctgtaat agtcacaggt gctatgagaa gtagttctga acttggatat
     2161 gatggttcaa gtaatttatc tgcatcagtt tgtactgcag tatccaaaga tgctatggga
     2221 aaaggagtcc ttgtagtttt aaacaatgaa gtattattag cttctgaagc tactaaaaca
     2281 aatactcttt ctctaaatac atttaaatct ctaactagtg gtccattggg aataattgac
     2341 tgcaacgaac ttgtccttac aagagatatt gtaaatagaa caattataga tactgataag
     2401 gtagaatcta aagttgcctt atttaaagca tatgtaggag aaaatgctga ctttataaga
     2461 tttgctgttg atagtggtta taaaggtata gttattgagg caatgggcag aggtaatatt
     2521 cctcctcaaa tgctttctgg agttgaatat gctagagaaa aaggacttcc tgtagttata
     2581 gtttctagat gtcattcagg tagagtattc gatagttatg gttactttgg ttctggtaga
     2641 gatttaaaaa atcttggttg tatatttggt ggggacttac caggtcaaaa agctagaata
     2701 aaacttatct tagcacttgg aaaaactgat aaccttgatg aaatcaaaga cttctttgaa
     2761 aaaggaatct attgttaatt ctaataactt ctatttaatt atttattgat atttcaacat
     2821 ttttaaatca aaatctaata cttatgtcag ttaaatgtca gcaaaatatt ttatccacaa
     2881 attttccaca acttatcccc aatattatcc acaagaaaaa aggtagcact tatattagta
     2941 gctacctttt atttattaat cttcatcttt ccagcttttt atccaatttc ctatttctat
     3001 tattttttct tcagttattt cttctccatc atgtaatttt gaaattagtt ctgataatgg
     3061 attgttatgt agacctccaa aaattctttt tccctctatt ttgagatatt ctttctcttt
     3121 tattaatact ctatagtgta catgtttccc aattctctgc gatatgaaaa aacctttttt
     3181 agttaatcta gctaaaagtg taattgtagt tgtatgcttc caagcatatg tttttctatt
     3241 taatctgcaa catcttttga tattacagtt ttataaccag tattccatat gtatttcata
     3301 atttttaatt ctgcatctgg taatttttta ttcaacataa tatattcctt ctttcaattt
     3361 atgtcacttc ctcaaactta tctagcttaa attaataata ggagcttttt atcacaaaaa
     3421 ctacaggtaa atttttctat atattctacg tatttagtct gtttttaagt gtgaaattac
     3481 gttcatacct ataaatttat tcaattttca aacaatcttg tttaataata actaattcta
     3541 ttgtattatt tagttataga ataaaactaa atcggcaaaa ctagagaaat atagtgacgc
     3601 aaagctatag ggactaagac ttatataaat atcttatgag ttatgtcagc cagttgccaa
     3661 agagatattg ttctttttgt ttttatgaaa gttttttaag gggataataa tttatatgtt
     3721 tagagataaa atggataaat gtacacatat attgactgct tatattagca gttcgtatga
     3781 ttattgtaat tttttggata caatttacat tccatatagt taatataaaa ctcaattaaa
     3841 ttatgacctt ggtttagata gttcattatt tacattccat atagttaata taaaacgtaa
     3901 ttcattagaa cctttaagtt gtaaatctct catatttaca ttccatatag ttaatataaa
     3961 acgagcatca aacacatgaa aatcggaaag cctacccatt tacattccat atagttaata
     4021 taaaacgtgg tagcgttcaa aatataataa aaggtgctac aggatttaca ttccatatag
     4081 ttaatataaa actagcgata gggttaaata acccaccata atctatcgaa tttacattcc
     4141 atatagttaa tataaaaccc caaaataaat tgagtatttc caatgcctac acatatacaa
     4201 atctctcaaa tttgcagtga accatgagta gtgcaattga taacacttat cacaagtcct
     4261 caatgcctta tatttcaact gttaagcctt aatttcttca aaaaatcgaa cactgcaaaa
     4321 tttctatgac tttattatat cataaaaata ttatttttga atatctgtac caatttgtga
     4381 tataataaaa gcaagaagaa ctacaatcta ttttgctgta gagtggagtt cataatttag
     4441 aaattactta gttttatgga atctgatttt taaatcaaat tcccaaccac tcttagttgc
     4501 cgctttgagt ggttttttac gctttctaaa tagtttgcta gctatataaa ctattaagct
     4561 agcagataga cttgctagta tgccttgtaa aaaattatcc atgtatattc acctcccttc
     4621 ttagcgtcgg gaggataatt ttttgataca tgaactccac tctataaatt gtagattaca
     4681 tcttcttgct aaaaatatta taacatataa ttcttacata ttttacctat ttattacttt
     4741 ttacattata ataatattct tttaatcaaa aaaacaccta ctattaaagt aaatgttttt
     4801 aagtattttt aattttaaaa tccatatagt tcatatgaaa ctaaatataa taactcaaca
     4861 tatgagcaat atcgagacat ttacatacca catagttaat ataaaacgaa aataaaaaaa
     4921 ttaaacttat aaaaccctta ttaatttaca tacccactag ttatttatat tcaaaaaaat
     4981 cttctccatc tgggttataa aataatattc tacttgccaa tccttgttca tatttataat
     5041 tttctttatc acctacataa gaaaatgtta atgtcatgct tcttgatgga tctaatgaaa
     5101 ttatatctaa ataaatagta catttttctc ctggctttaa tacaccgaaa ctattaaaat
     5161 ccatttttat tttagcatat tgccccatat ataatgttga attagcttct ccataaactt
     5221 ttaattttgt tacatctaaa ttttgtgtac ttgtattttc tatatttata ggaactgttg
     5281 ggtctttata tttttcagta taatattcaa ggttatcatc ataacattca agataacttc
     5341 ctttatcttg ccaaattatg tgctttgaat caaaatcaat ctttaatcca catattatat
     5401 ttttatttga agacggaaga ctaatattat tgatggtatt ttcacactct agcataattt
     5461 tatcacttag atttcctatc ccagtcactt ttgcagcatt tttaagtata tctttattac
     5521 ttccaatatc aactagtaca ataggattat ttttagccat tggtgaagca actaaagcat
     5581 aaactaaatc agtaccacta gctatataaa attcttttgc gccattatag aacttattta
     5641 ctattttttt attagtatca tatctatcta ctccacctaa tctagttgaa ttagtatcat
     5701 taactagttt gtcactcatt gatgaagtac cacctattac ataactttct acaccagttg
     5761 tattaaatgg aacacttttt ccatcagtta atactatagc tgctttatct ctaactgcta
     5821 caggtgcagc actcattgca tcaggctctc ctttaaaagc attagtcaat ataactttat
     5881 ttactttgtt aattgagttt atttcttttg caacattata gcttgtctta attctatcac
     5941 taccttgaag tcttttagcc tctattcctt tatcttttag aagagtttct gttgctttat
     6001 caatagaact ttctccacct attatgtata cctttttagc tttctctact cttttaagag
     6061 ttgcattagg tatgttattt ttcttagtta aaaaaattgg tgcatttgtt gctcctgcaa
     6121 gtccactcgc acttaatcca tcagccattg tgctgtctgc attaattaat atagctgtag
     6181 tataattttg ttcatcagct ataagagcag ccgtttcata tttatctttt ccttgaatag
     6241 tataaattgt atcaagtgca ttagctgata taggaccagc agcaaacatt gacacggcta
     6301 accctagtga taataatttc tttaatttca caatatctcc cccttttata tatgaaatac
     6361 tatatatctt atcataattc tacattaaat tttcttttcc tcctttaacg atatttatct
     6421 tcctgttaat ttaagtgtat acaaatacgt gttataatgt aactgtaaag aattaaaaag
     6481 ataatgacat aattagagca gtccaactgt tcttttataa aactaactta taaagggagc
     6541 gtacatatat gaatatcaaa tcagctttta taagaaaaag atgggaaaaa ttctatgtat
     6601 atgtggaata agaaactggc aaaaagaaac aaaaaagtta tggaagctat aaaaagaaaa
     6661 aggatgctga aaaacattta attgaaataa agtctactat aaataataat aagtttgttg
     6721 ctccaaaagg tatgacattt gtagaaagat gttataaata tatagacatg aataaaaata
     6781 attggtctcc atatactgtt acaaatacaa aatcttgggt taaaaattac atagaacctt
     6841 ttttaagaat ctaaaactca cagaagtaaa tcctagttca gtacaagctt ttgtaaatta
     6901 taactttaat aattctacct ttactagtgc taaagtaagg tatagctttc taagttctgt
     6961 aataagggaa gcgtatagat taagagaaat acaagaaaat tcatgtgatt ttataaaact
     7021 tccttctaaa gaaaaatatt ctacaaatga aatatacaat agagaagaag tattattgct
     7081 aatagaaaag ttacttggta ataatataga aatacctatt cttttaatgt tacttctagg
     7141 tttaagaatg tgagaagcta ctgggcttag gtggtcagat gttgacttag ataacaatct
     7201 gataaatgtt aatcaaattc ttgtctattc taatggcaaa atatctttta aagagccaaa
     7261 aacaccaaaa tcaaaaagag ctttatcagc tccaaaagaa ttaattgaaa aattgaaaat
     7321 tgaaaaacta agacaaagca aattaaaatt gtaaggtatc ctggaaaaca gaaataactt
     7381 agtttgtcta aacaccaatt taaatccgtt tctaccttct tcactaagaa aaacgtttca
     7441 caattttaca aaaaaaacaa tttaaaaaag attagaatac atgatttaag atatacaagt
     7501 gccactctgc tgcttttagg gggaactaat atgaaagttg tttctgaaag attgggtcat
     7561 acagatataa aaataactat gaatagatat tctcatgtac ttaaagaaat ggacaaagaa
     7621 gcttccgaca atctaagcga acttctattt aaatagtaaa aactgactgt cagttaaatg
     7681 tcagttaaac ctaaaaaaat aggttttgtc agttgattat cagtcaatta ttttataata
     7741 gtttcactta aaaacgtcta tatctacaca ttttactatt tgtttatgct tattaatatc
     7801 tatctgttat tattaattct aatagcttct atttaattat ttagttcagt ttacaggaaa
     7861 ctacacacaa accataaaat aggatgttga caatataaca gatagtatgc tcctgtaaaa
     7921 ggtaacatac tatctgttat gttgtctgga tttccaaatg gttacccttt ggcacacgac
     7981 tttgctatct ccttggtaaa ctaaaagttt gtaatctaaa atcctaatcc atctctataa
     8041 aatttaattg acttctccat actttttaca cctaaacata taaaagtaat tttattcact
     8101 taatgacctc cataattccc aaattagcta tttggaactt aactaaaaaa tcctaatata
     8161 agggcaacta ttataatggc aatcggaata catactgtgt aatcctgttc catagagtca
     8221 tcatctgaat gtagttttac aattcgcaat ttagcaatca gtttgaagca gaaatagcct
     8281 attattgttc caattacgtt cgtgagtaag tcgtctatat cggttgctct gtgtaacgta
     8341 aaaagctgaa tagtttcgat aaaaagagat aatccaaaac caatcaagaa tgtatttctc
     8401 aaacgttcaa aggttttgct tataagcgga cataaaaaac ctaatgggac aaaaagtaaa
     8461 atattcaaaa taaaactcaa actaaatcca tcactaaaag gaataagatt tatgtttgga
     8521 ttaaagaacg tttcccctaa ctgtgacagt cttatatatt caccgaatgt aggtatacca
     8581 acaatatgcg atagtgttaa acataagtag taatacattg ccagtgataa aatagatgtt
     8641 ttgacctttg gaatatcact atttttcttg aatattacaa ctaagaaaat accaatcaag
     8701 ataattccca acaagaaaat aggattaatt aacaataaat ctttgatagc ttcaaacaga
     8761 ctgagttgaa tttctacacc tgattgatta tttgttacag tctccatttg tacatacctc
     8821 ctcataacat ttcaattatt tctaaaaaaa atagaagata aatcttaaaa tcatcctatc
     8881 aaaattttta aatttgcctt tatcagttta taccaccagt taattcctgt cttacttctg
     8941 tatggtattc ttgggattct ttcatcaaat tagacaaaaa atggttacca tatcaataaa
     9001 ctggttacgc tccatatctc ccgaagcaac catctcctga gaataaatcc gtatcaggaa
     9061 tctccacatc tactgcacct atcacaagtt atttgataac atcctcaaac aatcccaact
     9121 tggatatatc gacaacaccc attagaatgc tactgctgac catccttgtt ttcctggtga
     9181 taattttaat caactggtaa gttgaaacgc ctattttttt tgcaagttcc tttttagaaa
     9241 catggtagct cttatatgaa tctgaatttc gttgtctggg tgtttcatat aaagcataga
     9301 tacgcacctt ttttgcctat tttcattata tagaaaaaaa tttcaaaata caataattat
     9361 atttttttcg tttaagctta gagcagttcc ttgatgaaca acactttgga aactgaatga
     9421 tttacatacc acttaataca ttaactcgat ttaacattaa ttataataat atttaaaaca
     9481 acaattaggg tatataagaa tgaacttttt agttctttaa tcattttttt gtataaaatc
     9541 aaatctattt ttataatttc aacaatt
//
LOCUS       DACXMZ010000155         9446 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.156, whole genome shotgun sequence.
ACCESSION   DACXMZ010000155 DACXMZ010000000
VERSION     DACXMZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.156"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            85..366
                     /locus_tag="JD016_13975"
     CDS             85..366
                     /locus_tag="JD016_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000738.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDIVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            615..884
                     /locus_tag="JD016_13980"
     CDS             615..884
                     /locus_tag="JD016_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000739.1"
                     /translation="MRGITMENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQ
                     NGGIMDTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            929..1252
                     /locus_tag="JD016_13985"
     CDS             929..1252
                     /locus_tag="JD016_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000740.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(1546..2397)
                     /locus_tag="JD016_13990"
     CDS             complement(1546..2397)
                     /locus_tag="JD016_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAU5000741.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(2446..3609)
                     /locus_tag="JD016_13995"
     CDS             complement(2446..3609)
                     /locus_tag="JD016_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAU5000742.1"
                     /translation="MFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKLSR
                     FIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNPYD
                     YLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKNDEN
                     NYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVIDRK
                     DEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPKSF
                     IDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFKEK
                     YNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            4077..4994
                     /locus_tag="JD016_14000"
     CDS             4077..4994
                     /locus_tag="JD016_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAU5000743.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     ICSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(5123..6052)
                     /gene="pfkB"
                     /locus_tag="JD016_14005"
     CDS             complement(5123..6052)
                     /gene="pfkB"
                     /locus_tag="JD016_14005"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAU5000744.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDSVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLVEIDKYQDLVEVKKLS"
     gene            6321..7046
                     /locus_tag="JD016_14010"
     CDS             6321..7046
                     /locus_tag="JD016_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU5000745.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            7129..8283
                     /gene="nagA"
                     /locus_tag="JD016_14015"
     CDS             7129..8283
                     /gene="nagA"
                     /locus_tag="JD016_14015"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAU5000746.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQETPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKNPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            8574..8768
                     /locus_tag="JD016_14020"
     CDS             8574..8768
                     /locus_tag="JD016_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000747.1"
                     /translation="MIDNRYIFKCKNTKNHINFKDIVGVNKQDNTIEIFTNECTYCIY
                     LKVLSIESEEYLIDRLKSFC"
     gene            complement(8849..8992)
                     /locus_tag="JD016_14025"
     CDS             complement(8849..8992)
                     /locus_tag="JD016_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000748.1"
                     /translation="MNFNHNCKLEKIALEIMLNYSVNKLYSTQKIDAIITIVTVKLKF
                     NVL"
     gene            complement(8982..>9446)
                     /locus_tag="JD016_14030"
     CDS             complement(8982..>9446)
                     /locus_tag="JD016_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAU5000749.1"
                     /translation="NILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVESKSLFIK
                     DRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPFGYEKNI
                     SLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIYINEF"
BASE COUNT         3421 a         1277 c         1242 g         3506 t
ORIGIN      
        1 actttcataa taaatacata taaagatatt tttaagaata aaatttatta ttaaataaaa
       61 aataaaggag agtgatttaa tagcatggca tcaaaacaaa caccaagaaa caatttagaa
      121 aagtttttaa gaataagagt cttatcacat acaggcgata aaatacatat aaagttacct
      181 gtaaattttg caaagagaat gattgagaat aatgctttgg atttatttaa tggaaaagat
      241 gatatagtag atggtaaaaa agttactgaa atattagtta aagcatttga ctatgattta
      301 actggagaag tagttcactt ggagagaaaa aatggagaca ttattaaagt ttcaattgta
      361 caatagaatt attttatatg aattttattt ttgaatagtt ataaacaatt agaaatttgt
      421 aaaaaagtat aatatattat tagtaatggt acagaataca catttataat ttatagatgt
      481 gtattttttg tttaaaaaat ttaaatatat aaaactataa aggttacaat tttggaaata
      541 gatgtaaata tatgttgtat tgtataataa gagtaatata aaatctagta aaaataatca
      601 aataaattta gcttatgaga gggataacta tggaaaataa ggattttaaa ttaacaatat
      661 gtatatttgc ttttgtaatt ggagtagtag tattattctg ttgtataggt ttaggtgaat
      721 ttagtgcaga gcatattatt caacaaaatg gtggaatcat ggatactgac caatataata
      781 tttatctaga ccaatctatt tctcaatata gaaattttgg ttctatatta gctctgttag
      841 gaggaatagg gatattgttt gataaatata tcagaaaata ctagagatta tgttttgtct
      901 aaaataaatt cttatgagga gataaaaaat ggcaacagaa atatttactg taaaaattac
      961 acccttagag gcacaaaaaa tgataaaaga aaatcttgat gcagatttag tttttagtga
     1021 ttcttataat ttaggagatg ataaatttat actaattaca acttttgaga agtattatgc
     1081 aagaaataat agtgatgcag gattaatagt agtttatgaa aataccacag gaaaaacagt
     1141 tattaaagct acttcaacag gcagtgcaat tggaatttta aaaattgatt ggggtgcagg
     1201 tggaaattta cttaaaagag taaaaaacat acttgctgat tatattattt aattatttta
     1261 tctaactgat aatatgtcaa gaaaatctcg taaaagaggt taagaacatt tttataatat
     1321 atagataaat aagaatttga aggtaaagtt tttaaggtat aaagaaaaat aatataataa
     1381 aacgaattgg ctttataatt ctttataagg caaagagcac atttctttat ataattgttg
     1441 gatattaaat aatatgtaaa tacaccattt acatataatt aatataaatt tttcatataa
     1501 caaatatcct aaacttatct aaaataaatt taggatattt tatttttata ctctatttac
     1561 agacccacac actttaatct tttctttcac aaaatccttt actgattgaa gccctgctaa
     1621 tccatacttt ttagggtcta taacctcaga atcttcatct aaaacttttt taaccccttt
     1681 actatatgca attcttaatt cagttgcata gttaactttt gcaatacctc tttttatagc
     1741 ttcttttata atatcatcat gtattcctga acctccatgc aatactaaag gtattgaaac
     1801 tacattttta atttctgata acctatctaa atctatttta ggttcaccct tatacaatcc
     1861 atgtgcagta cctatagcaa tagccaatga agatattcct gttctatcga caaactcttt
     1921 tgcctctttt ggatttgtat aatcattact attcccgcca tctaaatcat cttcttttcc
     1981 accaactttc ccaagttctg cttccactgg tataagagaa ggactacatg cttttactac
     2041 tgatttagat atagatatat tattttcaaa gctttcatga gaaccatcta tcattattga
     2101 tgtatatcca cttcttaaag ctttcattgc aaggtcaaaa cttgaaccat ggtccagatg
     2161 cattgctaca ggtatattta ctctttctgc agcaaccttt gcatttgcta gataataatc
     2221 aagacctgca tattttacag tagatggagt agtttgtaat attactggag aatttaactc
     2281 ctcagcagct tctataacag caattaccat ttccatattt tctacattaa atgctcctac
     2341 tgcataccct tcatcttgtg cctttttaag cattgatgtt gttgttacta atgccacatt
     2401 gataccctcc caaattattt agttataata ttttaatctt atcttttaat attcatatat
     2461 tgttacacct tttactactc gatttactgt tcctgtagct gatggcgtat ctggcttatt
     2521 tccaaccttt accgatacaa acaatgctat agtttgtgca aacaatatgt atggcattgc
     2581 aagatacaca tctggtaatt tattatactt ttctttaaac tcaaactttg ttcctgagaa
     2641 gttattttca ttttctactg acactgcaca agtgaattta gcaattttat ccctttttat
     2701 ctcctctaaa acatctaaat catattttct tgtatataaa cagttagata caaattcaaa
     2761 tacaagtgta ttttcatcaa taaatgattt tggtccgtgt ctaaacccca taggagaatc
     2821 atacactgta gatatttttc ctgctgtaag ttctaaaagt tttagttgtg cttcttgtgt
     2881 caaaccacct aatcctcctg aacctaaata tacaactcta tcaaaatctt tatttattaa
     2941 ctcatgtatt tcatcttttc tatctataac atttcttccc atttcaatta ttgcatttat
     3001 atatgacttt tctacatcat cttctaaact atcgaatatt aacatagcag ataacatcat
     3061 acacgaaaaa cttcctgtca tggcaaagcc ttcatcattt gattttgatg gcattaataa
     3121 caagtaatta ttttcgtcgt ttttagtcat cttagccagt tctccttctg gattacaagt
     3181 tatagctaaa tgataaaaat tatctacaat cttattccct aaatttaaag cagccaaact
     3241 ttcaggacta tttccacttc ttgcaaatga aattaataat gttggtatat ctttttttaa
     3301 gtaatcatat gggtttgaaa ctatatccgt agtaggtatt gcttcaaata tgtattttct
     3361 tatgtcattt ttatttttaa ggtaaggaag tattgaattt cctatgtaag ctgaagttcc
     3421 agcacctgta aatataactc taaactgccc atgattatta cttattgtat ctatgaatct
     3481 acttaatttt tctttgttag atttatatat ttcataagtc tctaaccata attcaggctg
     3541 ttgttttatt tcattagttg ttattattgc acctaaatct tttagttttt gttcttctaa
     3601 tttaaacatt ttcattctcc cttcatactt gtaacattta catttggtaa tgaggttata
     3661 accactttaa actataaaaa aatatactct tttataattt tttgcttaga ttttttagta
     3721 ctacttaaac tcactataaa atatacgcta tagattttaa ctaattttat agtttatctt
     3781 taaaaattta ataaaatcat actttagtat ttttatttta cgataaaaac ttgttcagac
     3841 caaagtggta attaccacta atccaaatat aaattaattt gaattttttg tcaatacata
     3901 aaatatttat atcaatcaat tttatagata tcctacaaaa ttgctttatc aatatttctt
     3961 atttcgctaa tttacttata taagctttaa atttattttt tgatacaaag tttacaaatt
     4021 tatgtgtctg aattataatc aattctgtaa atataaaaac cttaggagaa agtattatga
     4081 aaaatataaa aatgggaatg ataggtcttg gctccatagc tcaaaaggcg tatttaccta
     4141 tattaactaa gtctgagaga tttgagtttg taggagcttt tactccaaat aaagttaaaa
     4201 gggaaaaaat ctgtagtgat tatagaatca tgccattcga ttctattgaa tccctagcta
     4261 aaaaatgtga ttgtattttt ctacatagct ctacagaaac acactacgaa attataaaaa
     4321 tattattaaa tttaggtgtt catgtatatg tagacaaacc tcttgcttca acagtaagtc
     4381 aaggagaaga acttatcgaa ttaagtacga aaaaaaactt aaatttaatg gttggattca
     4441 accgtagatt ctgtcctatg tacaaagaaa ttaaaaataa tgctacagaa attgtatcta
     4501 tcaatatatg taaacatgga ttaaattcct taagaaatgt taaattcgac agtactctta
     4561 tagatgacta tatacatgtt attgacactg ctttatggtt agcaaatgaa gatgttgaaa
     4621 ttagtggaga ggatttattt ttaacagaca ataagaatct tatatttgta agtcataaac
     4681 ttaaaggtaa aaatttctct ataaacacct ctatgcatag agattctgga acaaagttag
     4741 aacaagttga aattctttca aagggtaaga ttcaaagagt taagaatctt aatgtactgg
     4801 aaatagaaga aggtggaaac ctaactttaa agcaaagtgg tgcatgggta aacatattaa
     4861 aacaaaaggg ttttgaagat atatcaaatc attttattga ttgtatagaa aataatataa
     4921 aaccagctat taatggagaa gagtgtatca aggctcaaag attactagag aaaattataa
     4981 attcagtaaa atagatattt attataaaaa taggttaaaa atgaattttt ctaacctatt
     5041 tttatataag actatatctt ttatattaaa attaaaatct attacaacaa cattaaattt
     5101 attatattcc cattaaatgt atttaactta acttttttac ttcaactaaa tcttgatatt
     5161 tatcaatttc taccaaatct acaaatccag tttccatatt taaagcatta cttataccac
     5221 aagcatttgc aaatttaagt aaattttcta ttttataacc ccttaaaata cctacactaa
     5281 atccagccac tgtgctatct ccactaccta cagtatttat acactttaca actggtactt
     5341 ttacatcata tacactatct tcgtttaaat acaccatacc atcttttcct aaagatatac
     5401 atacattctc tgcacccatt tcaataagtt tttttcctga caagataatt tcctctcttg
     5461 attctatatt tattcctaat aaatgtctta tttcatctat atttggtttt attaaatatg
     5521 gttttgattt taaagctatt tttaatgctt ctcctgatgt atctaatata aactttatat
     5581 tatttgctct acatatattt cctatctttt cataataatc tatcggcata ttctcacaat
     5641 aactaccaga agctaccagt attttagtat tttttagtac ttcctctata tcattttcaa
     5701 atctctctaa atcgcttttt tctactattg gtcctttttc taaaaattct gtactaactt
     5761 tattgtcctc aattatgttt aagcaatttc tagtttcctg ctctattttt gtaaatttat
     5821 tttgtatatc tagttttcta agttcatttt ttataaattc tccattaaat ccgcctagaa
     5881 atcccatagc taaaggattt tcttttaaaa ttttacatac cttagctacg tttaatcctt
     5941 tacctccagc agttgcattc ttacttacta ccctttgcac ctcacctatg tttatattgt
     6001 tcactctata cattctatct atagatgggt tgaatgttat tactgtaatc atactagatt
     6061 ctcctcatta ttaattttta cttttctaaa caataaatta atctgtaaaa taaaaaatac
     6121 ctcatctata atatatccaa agtggttatt accaatttga atataaatta ttttacttgt
     6181 tttgtcaatg taccataatt ttataataaa ttaatagcct tacttattga attttgatta
     6241 aactttattc attgacataa ataattctat attctataat caaattgtgg taataaccac
     6301 ttagaaagga ctatattttt atgataaaaa gaaatgataa aagaccaata tatgaccagc
     6361 ttgttgagat attaagaact aagattgaaa atgagatgga accaaacgat agaatgcttt
     6421 ccgaaagaaa aatttgtgat gaatatggag taagtagaac cacagttaga ctagctatgg
     6481 ctgagttaga gcatatggga tatatatata aaagacatgg aaaaggaact tttgttgctg
     6541 cactaagtca aaattctcaa aacttaatgg aaagttatag ctttacagat tatatgagaa
     6601 cacaaggaaa aaagccaggt acaaaggtat tatcatttga ggtagtagaa agtacaaagt
     6661 atttctctga aaaattaggt ataactcctg gtgaaaaaat gataaaaata atgcgtttaa
     6721 gacttgctga tgaccttcct atgatgcttg aaagaacata tcttcctatg aaagaattta
     6781 taggattgac aaaagaaaaa atcgaacaaa aacctttata tgaaatattt agagaagatt
     6841 atggcgagat tattaaagtt gctgatgaag aattttcagc tggaataatc tctgataagg
     6901 aagctaagtt attggaagta ccaggagatt caccttgtct gaaattactt cgtaccacat
     6961 ataatgatga caatatagtt attgaattta ctttgagtgt cgctagaagt gataaatttg
     7021 tatatagagt aagacatgta agatagtaag ttaaataact ttcaagaaaa cagaatgata
     7081 ttataagcgt caatgccaca atgatagtat aattataagg aggttatttt gaaaagtttt
     7141 atttatgcag ataaattttt tatgaaacat ggaatcaaag aaaaaggtta ccttagtata
     7201 attaatggca aattcggtga atttcaacaa gaaacaccta atgttactga taatgttata
     7261 gatttttcag gtaaatatat tgctcctggc ttagttgata ctcacattca cggtctttta
     7321 ggtgcagatg tcatggacaa tacatttgaa gcaataaaca caatttcaaa aggcttatta
     7381 aaatatggag taacatcttt cctacctaca actttaactg attctgtaga tacactaagt
     7441 gattctgtag aaaatataag caatagcttc aatcgagttg agggtgcaaa aatacaaggt
     7501 atttatttag aaggtccatt ttttacagaa aaatataagg gcgctcaaaa tgaaaaatat
     7561 tttaaaaacc ctgacatcaa tatattaaaa ttatggcaaa aaaaatcgaa aaatttaata
     7621 agaaaaatag caattgctcc agaacgtaaa ggttcaatag aattaacaag atatgcaact
     7681 aaaaataata tatcagtttc tcttggtcat agttcagcta catttgagca agttaaacaa
     7741 gtcgttaatt caggtgctaa agtgtttgta catacatata atgggatgag tcctttaaat
     7801 catagagaac ctggaatggt tggggctgct atggtactaa aagatactta tgctgaatta
     7861 atttgtgatg gtcatcatgt aagcccaata gctgttaaaa taatgatgga tgctaagtct
     7921 agtaataaca tagctttaat tacagattgt atgagagctg gtgctatgga ggatggaaaa
     7981 tatactcttg gtaagtttaa tgtaaatgta aaaaatagtg ttgcaagatt aaatagtggg
     8041 agtttagctg gaagtgtatt aactatggac aaggctgtga gaaatatagt tgattggaac
     8101 atatcaactt tagaagatgc aataaaaatg gctacatatg tccctgcttt aagttgtaat
     8161 attgataatg tctgtggctc tatatatcaa ggtatggatg cagactttat agtaacagat
     8221 aatgacttta atatacacga aacttatata aatggtatct gtaagtacaa agttaataaa
     8281 taggttcaat ataaggttat ggaggctgtc taaaaagagt ctggaaatat ttaaagttat
     8341 ttctccaaaa tctgatttaa cacttttatt tccatatcgt taataaacta ttgcgataga
     8401 ggttatttta aacaaatata tattataagt atttttgtga taatattttc gataatatgt
     8461 acatttaaag taacttttct attattattt gtaagttttt atcttgaaca aacatataaa
     8521 tttttaataa agcatgagat aaaccctata tttcaattac taatgatgag attttgattg
     8581 ataacagata tattttcaaa tgtaaaaata ctaaaaatca tatcaatttt aaagatattg
     8641 taggtgtaaa taaacaagat aatactatag aaatttttac aaatgaatgc acttattgta
     8701 tatatctaaa agttttaagc attgagagcg aagaatatct aatagataga ctaaaatcat
     8761 tttgttgatt ttatatattt tatgttaaat ataaagcaga gtagttaaaa ctaccctgct
     8821 tttgtaatta aaatttaaaa ataataactc ataatacatt gaatttgagc ttaacagtaa
     8881 ctattgtaat aatagcatca atcttttgtg ttgaatataa tttatttaca ctatagttaa
     8941 gcatgatttc taatgctatt ttctctaatt tacaattatg attaaaattc attaatatat
     9001 atatatttat aagttgtatt gtttatattg tttattactt ttttaaatcc actagctgta
     9061 ataataaatg tcttttctgg tatccctggt gaaaagatta ttttatcatg gttaaatata
     9121 tcttgatcta ttacaagaga gatatttttc tcatatccaa aaggagatac acatccaggc
     9181 aaacattgtg ttttttgtgt taattcttct tttgaacata tagatacttt tccacctaaa
     9241 acatttttta tagtttttgg atctaatttt tttccttctg tggttacaaa tatatagtaa
     9301 tttccagacc tatcttttat aaataaactt ttactttcta ctccagtata ttgtaatttt
     9361 tctcttattt ctttagcttt ttcaaatgat aaaataggtt catggtcata ttcttcaaac
     9421 gttatatgat tttcttttaa aatatt
//
LOCUS       DACXMZ010000156         9273 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.157, whole genome shotgun sequence.
ACCESSION   DACXMZ010000156 DACXMZ010000000
VERSION     DACXMZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.157"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(114..767)
                     /locus_tag="JD016_14035"
     CDS             complement(114..767)
                     /locus_tag="JD016_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU5000750.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(1012..2877)
                     /locus_tag="JD016_14040"
     CDS             complement(1012..2877)
                     /locus_tag="JD016_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HAU5000751.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     KINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2882..5323)
                     /locus_tag="JD016_14045"
     CDS             complement(2882..5323)
                     /locus_tag="JD016_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HAU5000752.1"
                     /translation="MIKGVVFLVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVL
                     CSLLKDEIAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGI
                     NLDDLIEAEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQ
                     VEVPDNWLTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDI
                     PIIGYQNQCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYP
                     DDSNYAGKLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIP
                     ELMRILLDEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEE
                     INRRYVEELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEV
                     LKDFYQDEPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFK
                     NDSSVLQRLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNV
                     LHMYHELLDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLK
                     VVFLENYGVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEI
                     CEQVGKENMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEE
                     GRSIYNSLTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSD
                     RTISEYAKDIWFKRM"
     gene            complement(5316..6758)
                     /gene="glgA"
                     /locus_tag="JD016_14050"
     CDS             complement(5316..6758)
                     /gene="glgA"
                     /locus_tag="JD016_14050"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAU5000753.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6787..7917)
                     /gene="glgD"
                     /locus_tag="JD016_14055"
     CDS             complement(6787..7917)
                     /gene="glgD"
                     /locus_tag="JD016_14055"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HAU5000754.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSEKKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7919..9079)
                     /locus_tag="JD016_14060"
     CDS             complement(7919..9079)
                     /locus_tag="JD016_14060"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAU5000755.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         2915 a         1706 c          909 g         3743 t
ORIGIN      
        1 tctatcgtag tgccttagag tttttatttg tatttaaagt taaatttatt cttgtttttc
       61 ctttaatatt taatagtaaa acaacaaaat tataaaaaaa ataaagagaa actttataaa
      121 agcttctctt ttttcatctc tgctataggt atcgttttcc aatgttctct caaaactctt
      181 tcaacatttt ctaaatcatt ctccctcata gattttacta cttcaatatg ttgattattg
      241 ctgtatgtca acattttata taactcatct tcattatcac tagtatatga ctttgacata
      301 aaattatatc ttaaagagtt caatagattt actaacacct gattatctga tttagttatg
      361 tatatatcgt gaaattcatt acttaatttt aaatagctat tgtaattttt atactttata
      421 gagaccgcca tcatttctgt taattcttcc atttttacta ggtctgattc agttaaattt
      481 tgtaatgctg aagaaccggc taacgcatcc aatactccaa ttacggaata ggtgtctaat
      541 ttctcctttt cctgaaattc ttttactaca aatcccttat taggtatttt ttctagtaaa
      601 ttttcacctg ctaacctagt taatgcttct ctcgctggtg ttctactaac tccgatagat
      661 ttacaaatct cagattcagt aattctctga ttagcttcat attctccatt ttggattttc
      721 tttgatatat agtcatatac ttgagttgta agtggatcta ccttcataag acgttcctcc
      781 atattaagat tataaaagtt aaatctccct acaactttgg tagagagttc agttttatct
      841 tactaattta tataatatca caattatgat attttttata cttctttgta atattttgaa
      901 atactaaaat tcttatgata atttatacaa aaaaagtaga aaaagttttg ataaaacaaa
      961 actctttcca cattcacctt attttctatt tatttttcta aaaaaagata tttaatttac
     1021 tctaaaaatt ctaaatgatt tagagtctaa atctactttc aactcattat tttcaatttg
     1081 tatagtacta tttgcatttt taagactata ctttataggt atttcctcaa taaaactact
     1141 ctctatacat aatgatatac tttccacatt ttcacttcta tttactaaaa ttaacattct
     1201 atcattattt tcattaactc taataaaaga aaatacagaa tcattattag tatatataaa
     1261 ttctgtattt ccagaactta aaagtttatt cttatttcta atttttataa catctctata
     1321 aaaattaagc atatcttcgt cttcattttt ccatggataa gttcttctat tatctgggtc
     1381 aacacctcca caaagacctg cttcatctcc ataatatata tatggtactc cttcaaatgt
     1441 catctgtata gctacagcaa gcttcactaa atctacatca tcatttaatt ccgttttagc
     1501 tcttgtaaca tcatgtgttc caattaaatt taaattagac ctaaatgatt cttttggata
     1561 attttcttta atctgcatat atctattaca aagttcatat gaattaattt ctccttttaa
     1621 gaaagaaaac atgttatttc taaaaggata acccataaca gaatctaatt cttcacctaa
     1681 taaataactt cttctctgac catagctaat cttatttgat gcatcttccc atacttcacc
     1741 tataagaatt gagtcatcat ctgccttttt taattccttt tttaactctc ttataaactt
     1801 agttggtaat tcgtctgcaa catctagtct ccagccctta atacccatat ttacccattt
     1861 atttataacg ctatcattgt catatattat ataatccata tagctatgtt ctaattcatt
     1921 tatatttggc aatgtcttaa catcccacca acttttatat ttttgaggaa attcttcaaa
     1981 catataccat gaataatatg gagattcttt agattgatat gcccctaaac tattatagtt
     2041 tccatacata ttaaaatatt tactatctgc tccagtatga ctaaaaactc catctaatac
     2101 tattgatata cctttttctt ttgctttatc aataagttct ttaaatgtat cttcatcccc
     2161 aaacattggg tcaatttttt tatagtcacc tgtatcatac ttatgattac tagaggcttc
     2221 aaatataggg cttagatata gtatacttac acctaatttt tttaaatatc caagtttatt
     2281 gattatccct cttaaatttc caccatggaa atcccatctt ataacatccc cttgactatc
     2341 ctttatgtac ataggtgtat cttcccaatt tccatatatg aaactatttt ttttaggatt
     2401 atcaacctta ccactacgat ttccattatt gaatctatct acaaatatat gataaagtat
     2461 tccttctttg taccatgttg gtactttaaa atctttgtaa acagtgattt gatatttatt
     2521 aatatcacta taattatatt cacaagaata tccattatct ctgttttttc cataaaataa
     2581 agtctttatc tgaccatcca tttcaacatc aactttaaag taataaaaat aaacacctgt
     2641 accatcaaac ttttcaatct taccacaaaa gcactttcca agtttatcat tatctaattc
     2701 ttcatttaaa gttattgttc tcatttcttc ttctctattt atttttaggg aaatagactt
     2761 gatattgtat ccctcattta ctttaacaca aatggtcaat tcctcatcaa attttaaagc
     2821 tccaaacgga gccttaaaat ttttatccca agagttatat tctattacac tttgcattta
     2881 atcacatcct tttaaaccaa atgtcttttg catactcaga aattgttcta tcagaagaaa
     2941 aaaatccaga gttagctata ttaactaaag acattttatt ccatttttct ttatctctat
     3001 ataacctgtt tatatctgct tgagcttgac cataattttc aaaatctctc aatacaaaat
     3061 actcatcatt gtaagtagtg agtgaattat aaatacttct tccttcttcg ccaagattag
     3121 gaataaatcc atttattaaa tcatctacta ccctttttat atccatatta gaatgatata
     3181 ggtcaagtga agaatatcca ccatatttat tataatttag tacttgttct gcactaagtc
     3241 caaataaaaa catattttct ttaccaacct gttcacatat ctctacattt gcaccgtcaa
     3301 gtgtagccaa agttatagca ccattcatca taaacttcat attacttgtt ccagatgctt
     3361 ccttagttgt agttgaaatt tgctcactaa catttgctgc tggtattatt atttctgcta
     3421 atgatactcc ataattctct aaaaatacta cttttaactt gtcctttact ctaacatcat
     3481 tatttatagt acttgcaaca gaatttataa atttaattat acatttagcc aaataatatc
     3541 ctggtgctgc ttttgcgcca aatataaaag ttcttgggtc aagattcaaa ttaggattat
     3601 caagtaattc atgatacata tgaagtacat taaatatatt cagtaactgt cttttatacg
     3661 catgcaatct cttaacttga acatcaaata tcgaacttgg gtctacattt aaatcgtatt
     3721 tatcttttat aaactttgct agatttgctt tattattgta ttttatatca tcaagtcttt
     3781 gtaaaacaga ggaatcattt ttaaattttt caatattttt taattgtaat gtatctttct
     3841 tccatgaatc accaattaaa tctgtaatta aattgctaag ttgaggattt gaacttataa
     3901 gccatcgcct atgtgcaatt ccattagtct tattattaaa tttatttggt tcatcttgat
     3961 agaaatcttt taatacctct gtttcaagaa tatgagtatg aagcttagca actccattta
     4021 cactatgact tgtgacaata cataagtttg ccatattgat attgtcacag tctattatac
     4081 tcattctttt tattctatct tggtcatatc ctttattatt taattcctct acatatcttc
     4141 tatttatttc ttctattatc atataaattc ttggtaatag ctctttcatc atacttacag
     4201 gccatttttc cattgcttct gacattatag tatgattagt ataggacact gtttttttag
     4261 ttatttccca tgcttcatcc caagataaat tttcttcatc aagcaaaatt ctcataagtt
     4321 ctggtatgca taaggtagga tgtgtatcat ttatatgaat tgctacctta tcgaataaat
     4381 tattaatatt taatttgttc tttttatatt ttcttattat atcttgtatc cctgcactta
     4441 caaaaaagta ttcttgtttc aacctcaaca acttacctgc ataatttgaa tcatcaggat
     4501 ataatacctg tgatatttcc tcagtataat acttatgttt taaagcctct tcatagctac
     4561 cagaataatt tagagcattt gaggtaagtt caccaaaatc tctctttggt atttcactct
     4621 taaacaatct taaagtattt atacattggt tttggtatcc gattattggt atatcataag
     4681 gcattgccat tacaggaagg tagtttttat gaattacttt taaatgacta ccttccttga
     4741 ttaattctac ctcaccacca aacttcacca ttgttgcttc atttggtctt acagtttccc
     4801 atgcatatcg tccttctgta agccagttat caggcacttc tacctgataa ccatttacaa
     4861 atttttgttc aaacagacca tatttatacc ttattccata tccttgacca cttatgttca
     4921 aagatgccat agaatctaaa aagcaagctg ctaatctacc aagtccacca tttcctaagg
     4981 ctgggtctac ttcagcttca attaaatcat ccaagttgat tcctaattca gataaacctt
     5041 ctctaacttc ttcttcaata tttaaattta ataaattaga ttttaactgt ctccctatta
     5101 aaaattctaa actaaaataa taaacttctt ttttcttatc taactttgta gcaacccatt
     5161 tttttgctat ctcatctttc aatagactac ataatacatt taacaattgt tcatcagtag
     5221 cttccttgat agattgtgca tataagctat acatttttac ttcaaatttt tgcttgaatt
     5281 tctttttgct tattgttacc aaaaaaacca ctcctttaat cacatatgtc tctatatgtt
     5341 tctatatact ccttagctga ttttttccaa ctattatctg ttttcattgc attttcaact
     5401 aatttaatcc atttttcttt gtccttaaat actttgattg catttttcaa acagaaaaac
     5461 atctcatgag cattgtaatt tttaaaacta aatccattgc cttctccagt aaatttatta
     5521 taaggtgtta ctgtatctct aagccctcca gtctctctaa ctattggtaa agaaccatat
     5581 ctcatagcta acatttgacc tataccacaa ggctcaaata atgatggcat taagaacata
     5641 tcacttgcag catatatctg ttgagccaat gatgcattaa aagtaattct agctgatact
     5701 ttatctgaaa aattagaatc gtaatagttg aacattgatt gatattgatg ttctccagtt
     5761 cctaaaacga caatctgtaa atcttctctc ataagttctg gcatcatgta tgctattaaa
     5821 tcaaatcctt tttgagaaac taatctcgaa actataccta tcaacggaat agatgaatct
     5881 ttttttagtc ccaatatatc ttgtaatctc aatttatttt ctactttttt gtcaattgag
     5941 tctacatcat aatgaacaaa tatatcttta tcttttgcag ggtcatttaa atcataatct
     6001 ataccattta atataccttt taattttcct gactcttttc taagtaatcc atctaattgt
     6061 tcgccataaa aagatgtttg tatttcattt gcataagtag gactaacagt tataatttta
     6121 tctgcaaaat ttataccagc tttcataaaa tttattgagc cataatactc tatatctcca
     6181 ttatcaaagt atttttgtgg taaatccaat atatcatata aaacatgctt atcaaataca
     6241 ccttgatatt ggagattatg tatagtatac atagttttta tatttttata cccctcaagg
     6301 gttgcatatc tttcttttag aattaatggt aacatccctg tatgccaatc atttatatgt
     6361 attacatctg gataaaaatc aattctactt atagcttcta aaactgcatc tgtaaagaaa
     6421 gtatatcttt ctgcatcatc tccatatcca tagatactct ttctttcatc ttctcttcta
     6481 aaataaaatt cattatctat aaagtaaaat tttacaccat ctaattccat ttctaaaaga
     6541 ccacagtatt gatttctcca tccaacacgt acactaaaca cagctatttc ttttagttga
     6601 tcttttaaat aacttggtat agttgaatat ttgggcatta ttacccttac atctacacct
     6661 tcttttttta attctttagg caatgcataa gaaacatctc ctaatcctcc agtttttgca
     6721 aagggccaac actctgctgt tacataaaaa accttcatta gtcattcccc cttctaaaaa
     6781 atttatttat atatttgtat tcttttccac tacaagagga tatttttcat ctcctcttaa
     6841 ttctttccca tttgatacac tcgtattttt atctaatatt gcattatata gttttgcatt
     6901 tgactttatt acacaatttt gcattactat tgagttctct ataactgttc ctttttcaac
     6961 atttaccttt ctaaatatga tactatcttt tacagtacct tctattaaac aaccagatgc
     7021 tataaagcta ttttctactt tagcactatc tgtataaatt gtaggtgatt cattcttttc
     7081 ttttgtaaat atcttttttt cagaatataa taattcatct gcaacttcta aatttaataa
     7141 atctttgctc atttcaaaat atgattcaac agaatttaca cattttagat atcctgtata
     7201 ttcataggct cctattttca tgttttctgc ttcttcaata ataaattctt ctaaataaca
     7261 ataattgcct ttatttacaa tgttatatat acattcaaca aaatcttcac gcttcataat
     7321 atatgtctcc atacatatgt ttgctctagg gaacttacct aaattatttc ctacatttat
     7381 taccctattg ttactatcta aatttagagt taagttacca gcaaaatctt tatttgcatt
     7441 atttacattt ttatatatta tagttatatc attatgagat tttttatggt attccaatgc
     7501 ttgagaataa tttaaattgc atagcatata gcttggagct attaaaatat attcttcttc
     7561 actcttttct atataatcta gattagctaa gatagtatat atatcacctt ctctatgtga
     7621 gtaagtattt ctatacttag tgttttctgg actaaaaatt gataggccac ctttttttct
     7681 acttaaatcc caatctttac cactacctat atggtctgtt aatgatctgt atttttcttt
     7741 tgcgaatatt ccaactttag tgattcctga atttatcata ttagataaag caaagtctat
     7801 tattctatat cttccagcta ttggtgtaga tgctattggt cttccatagt taagtttgtt
     7861 tattgctggg tctccttttt tgtttaaatt tattatccct aaacattcat ttctcatctt
     7921 aaagacctcc ttctattaat acgcatctag gttcgatatt ttcatactca gaaactacat
     7981 ttatttcatt atttacattc tttatctgaa cattatcatt tatagttgca cactcaccta
     8041 tcatagcttt ttctatagtt acattttcac ctattacaac atttggcatt attactgaat
     8101 caattacttt acttccttta ccaacaacaa ctccatgtga taatacagag ttacctactt
     8161 cacctaaaat tctacaacca tcagctatca ttgatctatg aacatttgca tttttaccta
     8221 tatattgtgg aggcattgcc atagtatttg tatatatttt ccacttaggg tctgctaaat
     8281 caagtgtttc tggagatttt attatatcca tattagcatc ccatagactt tgtatagttc
     8341 ctacatctct ccagtatcct ttaaatggat aagcatacat tccaacatta tcttctaaca
     8401 ttttaggtat taaatttttt ccaaaatcat cataatttat ttctggattt ttttctgctt
     8461 ccttaaaata atttctcaac attttccaat caaatatgta tactcccata gatgccaaat
     8521 tgctcttagg ttcacttggt ttttcttcaa attcatagat acttgaatct tcatttgtat
     8581 tcattatccc aaatcttgaa gcttcatccc aaggaacttc aatcactgct attgtagctt
     8641 tagagccttt ttccttgtga aattttaaca ttttgctata atccatcttg tatatatggt
     8701 caccagataa tatcaataca tattctggat tataggtatc tacaaagtcc atattttgat
     8761 atatcgcatg tgctgtacca ttataccaat ttccttcttt ttcattcata aatggttgta
     8821 aaacatagac accaccattt attctgtcca aatcccaatg acttcccatg cctatatgtg
     8881 aatttaatat caacggtcta tattgagtta aaacacctac agtatcaata ccagagtttg
     8941 aacaattgct taacacaaaa tctataattc tatacttacc tccaaatgat acagctggtt
     9001 tggcaattct ttttgtaaaa acacctaatc ttgaaccttg ccctcctgcc aaaatcatag
     9061 ctaacatctc ttttttcatt taatgacact cccctcatct agtaattaat atttataatt
     9121 gcatttttac ttttataaac tttatcagtc aataaatgtc gtgtataaat gttgcttaaa
     9181 taatacctta ataaaaataa aataatatca actatacaat tgatataatg tataccccat
     9241 aatggactta ccgacgtata cattattttc cat
//
LOCUS       DACXMZ010000157         9262 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.158, whole genome shotgun sequence.
ACCESSION   DACXMZ010000157 DACXMZ010000000
VERSION     DACXMZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.158"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            85..1845
                     /locus_tag="JD016_14065"
     CDS             85..1845
                     /locus_tag="JD016_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAU5000756.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            2126..2257
                     /locus_tag="JD016_14070"
     CDS             2126..2257
                     /locus_tag="JD016_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAU5000757.1"
                     /translation="MTLSEKKIILIITNYVGFNLLNFSVKGLPVEELRFKIINKKIM"
     gene            2274..4154
                     /gene="prdA"
                     /locus_tag="JD016_14075"
     CDS             2274..4154
                     /gene="prdA"
                     /locus_tag="JD016_14075"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HAU5000758.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            4235..4519
                     /locus_tag="JD016_14080"
     CDS             4235..4519
                     /locus_tag="JD016_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000759.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            4557..5282
                     /gene="prdB"
                     /locus_tag="JD016_14085"
     CDS             4557..5282
                     /gene="prdB"
                     /locus_tag="JD016_14085"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5007..5009,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HAU5000760.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            5362..6120
                     /gene="prdD"
                     /locus_tag="JD016_14090"
     CDS             5362..6120
                     /gene="prdD"
                     /locus_tag="JD016_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HAU5000761.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            6135..6602
                     /locus_tag="JD016_14095"
     CDS             6135..6602
                     /locus_tag="JD016_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HAU5000762.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            6620..7102
                     /locus_tag="JD016_14100"
     CDS             6620..7102
                     /locus_tag="JD016_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HAU5000763.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            7122..8129
                     /locus_tag="JD016_14105"
     CDS             7122..8129
                     /locus_tag="JD016_14105"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HAU5000764.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            8249..9130
                     /locus_tag="JD016_14110"
     CDS             8249..9130
                     /locus_tag="JD016_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAU5000765.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         3630 a         1145 c         1862 g         2625 t
ORIGIN      
        1 atttaataaa taattattat aatatgctag aataaatata tataaataag ttttgatgag
       61 gtactactat aaagggggga ctttatgttt tcgataccag aagtaaaaaa agtagaagag
      121 gtaatggata caaaatttac aactatagac gaagacacaa gaattgaaga tgcaataaaa
      181 gaaatgataa aaagcaacac aaaaactcta atggtaatag attcatctga ccaattaaaa
      241 ggcattatat ccatgacgga tatacataat ctttatgaga tgcataaaaa gtatgaaggt
      301 caaccagtaa aacttataat gaagaaggat gtaatatatg tcaacgaagg tttaacctta
      361 gatgaatgta gagatataat gatattaaaa aatataggga ttcttcctgt tcttagagat
      421 aataaaatta ttggagttct aaaacaagag cacattaggg actatctata tatgcattta
      481 gaagattatg gattgacttt gaagtatatt ataggtcaaa ttaaagaagg aatttgtgct
      541 ataaataacg aaggtgttgt tatcttgtgg aataagttta tggaggaaag gtatgatata
      601 aagtctgaag atatagttgg tcgaccgatg aatgaatttt tggaaaatac tatttcagaa
      661 aaagtactaa atagtaaagt tggaatgagt gacttgtatt ttacagataa aaaagaaaat
      721 atgtatgcat tagttcatgc aaatcctata ttttataaag aagaatttat cggtgttgta
      781 tgtacagaag ttgatgttac agaagctaag atattagctc ttgaacttga aaaagtaaat
      841 gatacattaa aatatctaaa gaacgaagtc aaaaatttat ctaaaggtag ttttgataag
      901 attttaggta aaagttataa attggaaaaa tcgaaagcga ttgcaaaaca agtggctaga
      961 acaaatagta gtatatttat ttggggcgaa agtggtacag gtaaagaagt atttgcaaga
     1021 gctattcatg attatagtga gagaaaaggg caatttatac ctgttaactg tagtgccata
     1081 ccaaatgagt tatttgaaag tgagtttttt gggtatgaat caggtgcatt tacaggagca
     1141 agtaaaaagg gtcgaatagg tatatttgaa cttgcaaaag atggaactgt atttttagat
     1201 gaaattgccg atttacctct tagtatgcaa gcaaagttgc ttagagtatt acaggaaaaa
     1261 gaaataagaa gagttggtgg agataccaca ataaaaataa atcctagaat aatatcagct
     1321 accaataaag atttgagtaa aatggtaaag gcagaaaaat ttagagaaga tttatactat
     1381 agattaaatg ttgttgaaat aaagattcct ccacttagag agagaaaaga agacatagga
     1441 ttattggttc atagcttttt agaggagata tgtaaacaaa ataataagcc tgttcttaca
     1501 attagcaaag atgtaataga tatatttcag aattatagat ggaaaggtaa tattagagaa
     1561 ttaaaaaata cgattgaaaa tattgttgta ttgtcacaaa attctaaaat agatgtggat
     1621 gatgtaccaa gttatatgat ggattctaca aataatagta ctgaagaaga agagtatcct
     1681 ttagatttaa cgaaggcaac tcaaaaaatt gaaataaaga atataaccaa ggcacttaaa
     1741 atgtctaatg gaaataaagc taaagcagct aagatattaa atatacccag aacaactttg
     1801 tattataaga tagaccaata caaaatagat gtgtctaaaa tatgacacat gactaaattt
     1861 tgacaaaatg ataaacttta atttaaaacc tagtaaagcc aacgcttata taagtgttgt
     1921 gtatatagat gtgttaaaaa aaatgaaaaa gtgttgatta attgacactt tttttgtttg
     1981 agaaaatgtt tacaaataca aaaaataaca tattttaaat aaatatttat ataatatttg
     2041 gctaaaaata gcgatagatg aagaatctat tatagaaaaa atctttttgt taataagttg
     2101 gcataggaat tgcttataat ataaagtgac tttaagtgag aaaaaaataa tattaatcat
     2161 aacaaattat gtaggtttta atttacttaa tttctcagtt aaggggttac ctgttgagga
     2221 attgagattt aaaataataa ataagaaaat aatgtaagga ggtattttat actatgtcaa
     2281 taactttaga aacagctcaa gcccatgcaa atgacccagc agtttgttgt tgtagatttg
     2341 aagcgggaac aattatagcg ccagaaaact tagaagatcc agcaatattt gcagacttag
     2401 aggattctgg attattaaca ataccagaaa atggattaac tataggtcaa gtactaggag
     2461 ctaagttaaa agaaacttta gatgcacttt ctccaatgac tacagataac gtagaaggat
     2521 acaaagcagg agaggctaaa gaagaagtag tagaagaaac agtagaagaa gcagctccag
     2581 tatcagaagc agcagtagtt ccagtaagca caggagttgc aggtgaaaca gttaaaatac
     2641 acataggtga aggtaagaac ataagcttag agataccttt atcagtagct ggtcaagcag
     2701 gagttgctgc tccagtagca aacgttgctg ctccagtggc aagtgcagca gcagaagtag
     2761 ctccaaaagt tgaagaaaag aaacttttaa gaagcttaac taaaaaacac tttaaaatag
     2821 ataaagttga atttgctgat gaaactaaaa tagaaggaac tactttatac atcagaaacg
     2881 cagaagaaat atgtaaagaa gctaatgaaa ctcaagagtt agttgtagat atgaagttag
     2941 aaataataac tcctgataaa tatgaaactt acagtgaagc tgtattagat atacaaccaa
     3001 tcgctactaa agaagaaggc gaattaggtt caggtataac tagagttata gatggagctg
     3061 taatggtatt aactggtaca gatgaagatg gagttcaaat aggtgaattt ggttcttcag
     3121 aaggtgagtt aaatactact ataatgtggg gtagaccagg tgctgctgac aaaggtgaaa
     3181 tattcatcaa aggtcaagta acaataaaag caggaactaa catggaaaga ccaggacctt
     3241 tagctgctca ccgtgcattt gactatgtaa ctcaagaaat aagagaagca ttaaagaaag
     3301 ttgacaactc tttagtagtt gatgaagaag taatagagca atacagaaga gaaggtaaaa
     3361 agaaagttgt tgttataaaa gaaataatgg gacaaggtgc aatgcatgat aacctaatat
     3421 taccagttga gccagttggt acattaggag ctcaaccaaa cgttgactta ggaaacatgc
     3481 cagttgtatt atctccactt gaagtattag atggtggtat ccatgcatta acttgtatag
     3541 gacctgcatc aaaagaaatg tcaagacatt actggagaga gccattagta ataagagcta
     3601 tggaagacga agaaatagat ttagtaggtg ttgtatttgt tggttctcca caagtaaatg
     3661 ctgagaaatt ctatgtatct aagagattag gtatgttagt tgaagctatg gaagttgatg
     3721 gagctgtagt aactactgaa ggtttcggaa acaaccatat agatttcgca tctcacatag
     3781 agcaaatagg tatgagaggt ataccagtag ttggtgtaag tttctcagct gttcaaggtg
     3841 ctctagttgt tggtaataaa tacatgactc acatggtaga caacaataaa tctaagcaag
     3901 gtatagagaa tgaaatatta tctaacaaca ctttagctcc agaagatgct gttagaataa
     3961 tggctatgct taaaaatgct atagaaggtg tagaagttaa agctcctgaa agaaaatgga
     4021 atccaaatgt taaattaaat aacatagaag ctatagaaaa agttacagga gaaaaaatag
     4081 tattagaaga gaatgagcaa tctctaccaa tgagtaagaa gagaagagaa atatacgaaa
     4141 aagacgaaaa ctaaaattta gatactaact aagatttaga caataacata aatttagatc
     4201 caagtttaaa acagtataga gaggtagaat tataatggat aggttaaaat atatctcatc
     4261 agagactttt tatgaaggta ttatagttga catcaaaggc ggtggagtga ctatagacct
     4321 aaaaggtaga cttggacaat ttaaaatacc taatagaatg cttataactg actacgaact
     4381 taagataggt caagaagttg gattcatgct aagctatcct gaggtattaa gtccagagcc
     4441 taatcaagag tatgtagaaa atattaggag agaaaaagaa aaacaagcag aaatgcaatc
     4501 aaaacaagaa gaaaaataat aaataatcgt ttgaaaatag aagggagagg aaatatatga
     4561 gccttacaac agtacaagga cttcaatctg aaatattcgt tccaataaca cctcctccag
     4621 tttggactcc tgtaactaaa gaattaaaag atatgactat agctttagct acagcagctg
     4681 gtgttcattt aaaatctgac aagagattta atctagcagg agatactaca tttagagcta
     4741 tacctaatac agctacagtt gatgaaatga tggtatcaca tggtggatat gacaatggag
     4801 atgtaaacaa agatataaac tgtatgttcc ctatagatag attacatgaa ttagctgcag
     4861 aaggatttat caaaggagta gctcctatgc actatgcatt catgggtggt ggaggaaacc
     4921 aacatgtctt cactgaagaa actggtcctg ctatcgctgc taaacttaag gaagagggag
     4981 tagacggtgt agttatgaca gctggctgag gtacttgcca tagaactgcc gtgatcgtgc
     5041 agagagcaat agaggaagct ggaataccta caataataat agcagctctt cctccagtag
     5101 ttagacaaaa cggaactcct agagcagttg ctccattagt tccaatgggt gctaatgctg
     5161 gtgaaccaca taatatagaa atgcaaacac atatattaag agatacatta gagcaattag
     5221 ttgcaatacc atctgctggt aagatagttc cattaccata cgaatataaa gctcacgttt
     5281 aaaatgtaat ttatatttga aaatatagaa cccttctcct acacggttag gagaggggtt
     5341 ttaagaacaa aggtggatat tatggaagaa aaaataatga gaagacttgt tataaaaccg
     5401 tttcatatga atgaagttaa tttcggttca aaaacttcta taaagaaaga tgtacttaca
     5461 atagatttat caagtataga tgaaataaaa gaaagagaag agttaataac agacataaaa
     5521 gtagacatta taaaacctgg agattatgac agagaaatta atactattat ggacattata
     5581 ccaatatcaa caaaagtttt aggcagactg ggagagggta ttacacatac tttaacgggt
     5641 gtatatgtaa tgttaactgg tgcagatgaa gatggaaatc aaatgcatga atttggttct
     5701 tcagaaggaa ttttgaaaga ccaaatggta tttgggagat atggaactcc atcagttgaa
     5761 gattatataa tccatgtaga tgtaacttta aagggaggtt taccttttga aagaacactt
     5821 ccacttgcag cattcagagc atgtgatgat tttatacaag aaataagagc gtctttgaaa
     5881 atggaagacg gaagaaatgc tactcaagtt cgtgaatatt ttgacaaaat tcgaccaaat
     5941 gcaaaaaagg ttgtcatagt aaaacaaata gcaggtcaag gtgctatgta tgacaatcag
     6001 ttattctcaa aggaacctag tggatttgaa ggaggaacat caattataga tatgggaaat
     6061 gtacctatga ttatatctcc taatgaatac agagatggtg cccttagagc tatgacttaa
     6121 gagaggtgga tattatggga ataggacctt caacaaaaga aacatctctt catcacttta
     6181 gagacccact tttagacata gttgaaagtg ataaggatgt agatttactt ggagtaatag
     6241 tagtaggaac tccagatgga aatgaaaata aaacctttgt gggacaaaga acggccgctt
     6301 ggctggaagc tatgagagta gatggagcta tagtttcatc agatggttgg ggaaactcac
     6361 atgttgacta cgctaacaca tttgaggaaa taggaaaaag agatatacct gtagttggag
     6421 ttacatttaa tgggactcaa gctaagtttg ttgtaagcaa tcaatatatg gataccatag
     6481 ttgatatgaa caaatcaaaa gaaggtattg agacagaagt agttggagaa aacaacacaa
     6541 atgaaataga tgctaaaaaa gctttggcat ttttaaaact taaaatgaga aaacatggat
     6601 aaaaaattta agtaagggaa tgagttttgt gggaatttct acatcaatga aaaacacatc
     6661 acttcatata tttagagacc cgttactaga tttgatagat aatgattctg acatagactt
     6721 gttaggagtg gttgtagttg ggacatcaga gcacaatgaa tggaaaacat ttttagcaac
     6781 tagacttgga agatggattg aatcattgcg acctgatgga gtgattataa ctttagatat
     6841 ggcaggaaac cagcacattg attttacaaa tgctatagca gagtttgtaa aatcagatat
     6901 acctacagta ggtcttacta ttatgggagc agatggatta gtaattacca atccatattt
     6961 ggataaagct accataattg attataaaaa gacaacaggc tacatagaaa cagaagtagt
     7021 tggagacaat cacatggacg aagttgatgt taaaaaagca ttggctttcc taaaattaaa
     7081 aatgcgtaaa gacgcaaaat aaacctaagg gggaatttga tatgaaattt agcagaagta
     7141 tacaagctat agactctcat acagcaggtg aagcaactag aatagttgta ggtggaatac
     7201 ctaacataaa aggaaattct atgcctgaga aaaaagaata tttagaggaa aatctagatt
     7261 atttaagaac tgctataatg ttagagccaa gaggacataa tgatatgttt ggttctgtaa
     7321 tgactcaacc ttgttgccca gatgctgact ttggaataat cttcatggat ggtggcggat
     7381 accttaacat gtgtggccat ggtacaatag gagctatgac agcagctata gaaacaggtg
     7441 tagttccagc agtagagcct gtaactcatg tagttatgga agctccagca ggaataataa
     7501 gaggagacgt tacagttgta gatggaaaag ctaaagaagt atcattctta aacgtaccag
     7561 ctttcttata taaagaaggt gttgaagttg acttaccagg tgttggaact gttaaatttg
     7621 acatatcatt tggtggaagc ttctttgcta taatacatgc aagtcaatta ggtttaaaaa
     7681 tcgaacctca aaatgctggt aaattaactg aactagctat gaaacttaga gatataataa
     7741 atgagaaaat agaaatacaa catccaactt tagctcatat aaaaactgta gacttagttg
     7801 aaatatatga tgagccaact catccagaag ctacttacaa aaatgtagtt atatttggtc
     7861 aaggtcaagt tgacagatct ccatgtggaa ctggaacaag tgctaaatta gctactttac
     7921 atgctaaagg tgaattaaaa gtaggagaaa aattcgtata tgaaagtata ttaggaactt
     7981 tattcaaagg tgaaatagtt gaagaaacta aagttgcaga tttcaatgct gtagtaccta
     8041 aaataactgg ttctgcttat ataactggat ttaatcattt tgtaatagat gaagaagacc
     8101 cacttaaaca tggatttatt cttaaataag taataaataa attaggaaat taaaatagaa
     8161 tagtagccac ttgtcggttg ctattctata gcttttaggc tataagattc agaatatatg
     8221 aaaaaataaa aaaataggag gatggattat ggtagcaatc ttaaaggcat tatatgtagt
     8281 attcgcagca atctttgcag ttgtgtttgg taaagacgta gctaaagcaa acaaagaggg
     8341 gaaactagaa gaacaaagta ctccaaaagt tgctgtaaca ggatttataa cagacttctt
     8401 tgatacatta ggaatagggt catttgctcc aacaatagca atgtcaaagg ctttaaagtt
     8461 aaatatacca gataaaaaaa tgcctggtac attaaacgta gcacatacta ttccagttgt
     8521 tacagaagca ttcatattta catcaatcat accagttgat ggtgttactt tagtatctat
     8581 ggttgtggca gcagcagttg gttcatatat aggtgcagga ataatagcta agatggacga
     8641 gagaaaaata caacttgtta tgggtgtaac tttagcatta acagcaatat taatgttatt
     8701 aggtcatcct tggattaacg tattaccagg tggaggaaat gaattaggtc ttactggtgt
     8761 taaattagta ataggtgtaa taggtaactt tatactagga gctttaatga cagctggtat
     8821 aggtctatac gctccaggta tggcaatggt ttatttctta ggaatgtctg caaaagttgc
     8881 attcccaata gttatgggtt cttgtgcatt attaatgcca gttgcaagta tgaaattcat
     8941 aaaagaagat gcatatacta aaaaagcatc agttataata gctatatgtg gtttaatagg
     9001 agttttcgta gctgcatata tagtaaaaac tctaccaatg gatatattaa aagcattagt
     9061 aatagtagtt atagcttata cttctgctac aatgataatg gctgcaaata aaaacaaaaa
     9121 gactgcttag ttaaagacta cttagtgttt attaaatatt atagctaata ataaagatta
     9181 taaactgctt taaaatattg aattaatatt ttagggcagt tttttattta ctaaattaat
     9241 ataaagcaat taacatgcca at
//
LOCUS       DACXMZ010000158         9188 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.159, whole genome shotgun sequence.
ACCESSION   DACXMZ010000158 DACXMZ010000000
VERSION     DACXMZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.159"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            222..542
                     /locus_tag="JD016_14115"
     CDS             222..542
                     /locus_tag="JD016_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HAU5000766.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            680..1324
                     /gene="trmB"
                     /locus_tag="JD016_14120"
     CDS             680..1324
                     /gene="trmB"
                     /locus_tag="JD016_14120"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAU5000767.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            1453..2199
                     /locus_tag="JD016_14125"
     CDS             1453..2199
                     /locus_tag="JD016_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000768.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(2328..2606)
                     /locus_tag="JD016_14130"
     CDS             complement(2328..2606)
                     /locus_tag="JD016_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000769.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            2872..3327
                     /locus_tag="JD016_14135"
     CDS             2872..3327
                     /locus_tag="JD016_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000770.1"
                     /translation="MRYMESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKY
                     FESIGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVV
                     FGFIGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            3597..4049
                     /gene="accB"
                     /locus_tag="JD016_14140"
     CDS             3597..4049
                     /gene="accB"
                     /locus_tag="JD016_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAU5000771.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            4065..5426
                     /gene="accC"
                     /locus_tag="JD016_14145"
     CDS             4065..5426
                     /gene="accC"
                     /locus_tag="JD016_14145"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAU5000772.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            5441..6295
                     /locus_tag="JD016_14150"
     CDS             5441..6295
                     /locus_tag="JD016_14150"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAU5000773.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            6316..7260
                     /locus_tag="JD016_14155"
     CDS             6316..7260
                     /locus_tag="JD016_14155"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HAU5000774.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            7449..7709
                     /locus_tag="JD016_14160"
     CDS             7449..7709
                     /locus_tag="JD016_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HAU5000775.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            7862..8233
                     /locus_tag="JD016_14165"
     CDS             7862..8233
                     /locus_tag="JD016_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HAU5000776.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            8263..9108
                     /locus_tag="JD016_14170"
     CDS             8263..9108
                     /locus_tag="JD016_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000777.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
BASE COUNT         3566 a          955 c         1548 g         3119 t
ORIGIN      
        1 ataaaattat tatatttttt agaataataa ttttatttgt ttactaagtt tatgataggg
       61 tatttattta aggtaataaa atattatata aaatatttta aagccctagg taatattagg
      121 tagaagtttt agtaaaatat gcaaaaacta attgattatg taccttgtat gggtatataa
      181 tagatattgt tttaataaaa tatttgaaag ggaatgattt gatgaaaatt actttaccag
      241 aaacagctat tgatacttta aaaagtatct taaaagacaa tcaagataaa cctaataata
      301 taagagtata ctttgcaggt gttggttgtg gaggtccttc ttttggatta gctttagatg
      361 agaaaaaaga agatgactta acttatgaag ttggagagtt acaatttgta atgagctctg
      421 atgaatatag tcagtatgga gatataataa tagaagatac aggatttgga tttagagtta
      481 ttcctgaaaa tatgaaagat caaggtggag gaggatgctc tggttgttca gggtgccact
      541 aagattaagt ttttagtaag tttagaactc tctttataga aataaatttc tattttaaga
      601 gagttctttt tattttttgg tgagataatg tataataaaa atgtatttta ctaatttttt
      661 atatgtaagg agaattattg tgagaagaag acgaaaaaaa ggtgcagatg aaaagctttt
      721 aagttataca aaatatgttt taagagacga tatagacaaa ttaaaaggca agtggaattt
      781 aaagtttaga aatgataatc caatacatgt tgaatttggg actggaaaag gtaagtttat
      841 aacaacttta gctaagcaga accctgacat aaattatatt gccatggaac ttaaggaaga
      901 agttctttta aaggctgttg aaaaagcaga tgcttctaat ttgaataata tactattttt
      961 atggggagat gtaagcaata ttttggatta ctttgaagct aaagaattat cgagaattta
     1021 tataaatttc tgtgacccat ggcctaaaaa tagatggagt aaaagaagac tcactcattc
     1081 tggattttta gaaatgtaca atagggtttt ggaagatgat ggcgaaattc actttaaaac
     1141 agataatgaa aaattattcg agtttagttt aaatgaaatt gctgcaaata attggctatt
     1201 aaagaatatt tctcttgatt taggaaatag tgagtatgaa aataatgtta caacagagta
     1261 tgaagacaaa tttatgtcac aaggtatgcg aatatttaga tgtgaagcta aaaaacgtaa
     1321 ctagtaatat gtttataagt ttaatagtag aaatttattt taatttatat taataaagtg
     1381 tacgaaagta ggtatatata acaaggaaac agaaccctct catagaaatt aaattattat
     1441 aataaaaaat ttatgtggga gggtactatg aatttagcta atttaatacc atttattatt
     1501 tctgcagcag ctttaagtcc aataaattca aatgataagg ttttgagcga aaatcaactt
     1561 aaagatattt ttcctgatgc aaatttaaga gcagttgtaa aaagatatat aaacccagat
     1621 gagatgacta taagcaatat aaaggcttta gatggagaat tttatgcaac tggtgagagc
     1681 atttcaaatt taaaaggtat atcatatctt gaaaatgtag acaattttat tttttggaat
     1741 aataatataa aagaggttcc taaggaagct ttaagtctaa aagatatgga ttcaataaat
     1801 cttgccaata attatttgat tgatgacgat gtagtaaatt ctctttctca taatggtgtg
     1861 gatgtaaatt gcgatttaaa ttttattgat acaaaagata atcagtataa actggactct
     1921 aaatatcata atgttgatat agcaaaaggt gaaagtattg atttgagaaa aataattacg
     1981 aagaaaattg atagttatta taaatactgg gaagttactg ataaccttcc aaaagattta
     2041 aattttattg tatctgtaag tgataagagt gtactttcat gtgaagatat gataattaaa
     2101 ggtaataaaa caggtcaagc agtggtaaaa gttatactaa gcgacaaaaa tgatttaaat
     2161 acatctcaag aagttctaat tactgtcaat gtaaaataat ctgtcaactt attcaatcat
     2221 tctattttca atttaagtgt ataatcaaaa taaaggttgt tagtaacaga acgctaatat
     2281 tagtgctttg ttactagcaa cctttaaatt ttatagaaat agattatcta aaaaattcca
     2341 tttcttctgt tagtagccca tattcctatt aaaattatca gtgaatatat tatatacaaa
     2401 actattctta tatattggac atctaatcca aaaaattcaa tgttactctg tgaggtatgc
     2461 aacatcacaa ggactgcaga tataccaata agcaaaggat tgattactat ttgcttatca
     2521 gttcttaaaa tcattatttt acccatccaa attattgata atataaaaaa taaaatttta
     2581 agaccaaatt ctaatgtatc taccaaagcg aaaacatccc ttcatttata ataaattttt
     2641 cagctcaaca aaatataaaa ccctacaaac aacttttgaa tccataataa gataacttga
     2701 ttaagcacta tgaaataagt actataaaac ttcatctaca ttaatatata ctacactata
     2761 cttcaaattt cttaaacata aattaatgaa attaacaaaa ttttattcag aaacatataa
     2821 agatatattt tactatttaa atgcatatca ttatatatac agttttagga ggtgaggtat
     2881 atggaatcta aattttttaa ttggtacact caatccttag ggggaacttt tggcattata
     2941 tcatgtatat gcgcatattt aaatggatac atgtttgttt atacaaatag tgctagcaaa
     3001 tattttgaaa gtataggatt tctgggaata atttcaagtt atacactact tccactttgt
     3061 atgcttactt tgatttttgg agttatatat tcttatactc cagaaacatc cgacaaaagt
     3121 atttttaata tacctttttt tactttaaat aaaagattaa tacttattac agtagttttt
     3181 ggttttatag gtgctagagt ttactttata attcctgcaa ttctaataac ttttaaccta
     3241 tatagcaatg ttttgatagc taaattaaag tacatcctat gtaaaaataa agaaaatgat
     3301 actaatttga aagaaagcga agtctaaatt gcataatatt ggtatttgtc ttaaaaattc
     3361 ccgaggtatt aatttgtatt ctcttgtatt ttgcaaaaaa tattgaattt tattcatttt
     3421 atacttataa tttatattaa taatggaaaa ttaaaacaaa tataagttac tggttgttaa
     3481 tttaaaatta aaagatatat tgatgtgttg attctctagt cagcactttt tcttttataa
     3541 atttgttttt tgtgatataa tattagcacc aggtaataat acaagggggt aacaaaatga
     3601 acatcaatga aattaaggaa ttattaaagg ccatagactc aactaactta gaatatgtaa
     3661 aacttgaaag tagcgattta agattagaag tatctaaaaa agctcaatct acaagttcac
     3721 ctgttttaag tgtacaacaa gaatcagtag tggatctttc tttagaaaag ccagttgtaa
     3781 acgatacacc tgtcacatct aatgagaatc tatcagtagt agtagctcct ttgatgggaa
     3841 cattctatga ttctccaagt ccagatgcag atagctttgt taaagttggc gatgtagttg
     3901 aagaaggcga cactttatgt atattagaag caatgaagct tatgaatgag ataacaagtg
     3961 aaattaaagg tgaaataatt gaagtattag ttagtaatga agaactagta gaatataatc
     4021 aacctttatt taagataaaa ccattgtagt aggaggtatt taagttgata aaaaaaatac
     4081 tagttgcaaa tagaggcgat atagcagtta gaatcatcag aacatgtaaa gaactaggta
     4141 taaaaactgt tgctatctat tctgaaatag ataaagattg ttttcataga tatatagctg
     4201 atgaatctat ttgtatagga ccaaataata taagtaagag ttataataat atagaaaata
     4261 taatctatct tgctcttaaa ttaaagtgtg atgctattca tccaggcttt ggatttttat
     4321 cagagaatcc tgaatttgca aagcaatgtg aagataataa tattattttt ataggtccaa
     4381 caagagaaca aatgattttg atgggagata aatctagagc aagagaaact atgatggaat
     4441 tgaatatccc tgttgttcct ggttcagaat ctgttttgaa aactaaggaa gaagctttag
     4501 aagttgcaag agaaattgga tatcctgtta tgataaaagc ctcaagtgga ggcggtggaa
     4561 aaggtatgag aatcgttaga aaagaagaag aacttttttc aaattttgat atggcaagtt
     4621 cagaagctct agcagcattt tctaatagtg atttatatat ggaaaagttt atagaaaatc
     4681 ctagacatat agaagtacaa gtatttggag ataaacattc aaatgcaata catttaggtg
     4741 atagagattg ttctatgcaa aggcgaaatc aaaaagtaat agaagaatct ttaagtccat
     4801 acctttcaga tgaagaaaga caaaaacttc ataaaattgc agtagatata gtaaaaggag
     4861 taggttatat cggtgcaggt actattgagt ttatagttga caaagataaa aacttttatt
     4921 ttatagagat gaatacaaga atacaagttg aacatccagt tacagagatg gtaacaaatc
     4981 ttgaccttat aaaacttcaa atatctatag ctaatgggga taaaattcct tttaaacaag
     5041 aagatattac ttttagaggt catgctattg aatgtagaat aaatgcagaa gattcatcaa
     5101 agaattttgc tccatcacca ggtaagatag aatcattaaa tttacctgga ggttttggtg
     5161 ttagatttga tacttttgta tatgcaggat atactatacc acctttatat gattctatga
     5221 taggtaagtt aatatgttgg gctgaaacta gagaagaatg tataagtaga atatatagag
     5281 ctctagacga aattattgta gaaggtatta atacaaatgt agaatttcaa aaggcacttg
     5341 taacaagtga ggagtttaga gaagatactc atcataccaa atttatagaa gatgtcttta
     5401 tgaagaaaga atttgctaca ttataaggag taaatagatt atgataaaaa aatttttatc
     5461 atcaaaagaa tctaagtatg ttacgatttc tttagatgac aactttaaaa agaatagtgt
     5521 agatgataaa ttttggacct attgcaaagg ttgtgatagt catgtattta gaaaggacat
     5581 agaagaaaat tcatttgtat gtccaaaatg ttcaagacat tatggcttaa gagcaagaaa
     5641 aagaataaat ttattgattg ataaaggaac ttttatggag tttaattctg atgtagaatt
     5701 tcaaaatcca ctaaattttc ctaaatacaa agaaaaagta gactcttata aggaaaaaac
     5761 aaaagagtca gaagctgttg tcacaggtta tggaagaata aatggaatta aaacagttat
     5821 ttgtgtaatg aatcctgatt ttatgatggg aagtatgggt tctattgttg gggaaaaaat
     5881 aacttattca atagagtatg ctgctgaaaa taatttacct ataattattt gttctgcttc
     5941 aggaggagct agaatgcaag aaggtatggt atctcttatg cagatggcaa agacttctca
     6001 agcactttct aagcttgaag aaaaatcatt gccatatata tcagttctaa ctgaccctac
     6061 tactggagga gtaactgcta gttttgctat gcttggtgat attataattt ctgagccaaa
     6121 tacgttgata ggttttgcag gtccaagagt aatagagcaa acaataaatc aaaaacttcc
     6181 tgaaggattt caaacttctg agtttttact tgaaaaaggt tttattgaca tgatagtaga
     6241 tagaagaaaa atgaaggaag tattatatca aattcttgct atgcacaaga aatagggcat
     6301 aaaggaggaa ctgttttgat tgaaaataat agtgaaaaaa taaaagtctt agaaaatgat
     6361 attaaacaat taataactat ttctaaggca aataatatag atttaagtga taaaataaat
     6421 tcacttaatg agaaattgga aaagcttaag gaagatgctt tttcacatct atcagcatat
     6481 gaaaaggtaa cattaagtag agatataaag agacctacga cattggaata catagaacat
     6541 atttgttcta attttttaga acttcatgga gataggcttt ttaaagatga cccttctatg
     6601 gttggaggaa ttggtcagat aggaaagttt aatgttacta tagttggaca ccaaaaagga
     6661 agagatacaa aagaaaatat aaaaagaaat tttggaatgc ctcatccaga aggatacaga
     6721 aaagcattaa gacttatgaa acaagctgaa aaatttaata gacctataat aacgtttatt
     6781 gatacatcag gagctttttg tggtctagaa gcagaagaaa gaggacaagg agaagctatt
     6841 gcaagaaact tacttgaaat gagtaaactt tctgttccag taatcacttt tgtaattggt
     6901 gaaggtggta gtggaggagc tttaggtatc ggagtaggaa atgatgtttg tatgttagaa
     6961 cattcagttt attctgtaat ttctccagaa gggctttcta gtattctatt taaagattca
     7021 tctaaagcta aagaagcttg tgatgttatg aagcttacaa gtaatgattt atatgattta
     7081 aaaataatag ataagattat aaaagaacct cttggaggag cacaaaaaga tgtagaagct
     7141 gtttcaaaag aaataaaagc ttatatttta gaaagattaa atcattacaa agatatggat
     7201 aaagaggaaa ttatagctca aagatataaa aaatttagaa atattggaaa atgcttatag
     7261 aatataaaga aatttgtagt tttttcataa taaaatggtt aaaaaaaata aaggttttct
     7321 taaaacgatt atagaagtat atttttataa aagtatactt taaatgatga ttgtaattag
     7381 taaatggcaa ttatgattta gtactttagg taatggatac tttaacaaat taacaaggag
     7441 atgttgaaat ggaggtacta aaagtttcat caaaatctaa tcctaattct gtagctggag
     7501 ccttggctgg agtactaaga gaacgaggcg ttgcagaaat acaagctata ggagctggag
     7561 ctctaaatca agcgataaaa tcaattgcaa tagcaagagg ttttgttgca cccagtggaa
     7621 tggatttagt ttgcattccc gcatttactg atatagaaat cgaaggtgat aagaaaacag
     7681 ccattaaact tataattgaa cctagataat atatctcttt attagaattt aattaagttt
     7741 taaaggttgg ctatagaaat aggacactat agtgaacctt ttttacttta tatgttttat
     7801 tttatgtaac ttttaatgta taatcttaag taaacaaata agattggagg aaatatacat
     7861 tatgaaatta aaattattgg tagaagagta tgcagtatgt agattaaata atgacagtaa
     7921 aataccaaca tggatagata cagagaagtt ttactctata actagaactg atgatgaatt
     7981 aagtgttgta tgtttaaata ataatattcc ttctgatgtc aagtcagaaa aggagtggag
     8041 aatacttaag atactaggtc ctttagactt ttcacttgta ggaattttat ctaagttaag
     8101 tagtttgtta gcagacaaca aaataagtat atttgcaatt tctacttatg atacagacta
     8161 tatcttagta aaagaaaaag atatagaaaa tgcttgtaaa attttaagct gtaatggata
     8221 tgaagtagag tagttaaata cttttacatg aggacttaac ttatgattta tataatcgaa
     8281 attttaaaaa taagtgtaat aaatattttt tgtttcatag gaatatttat tgtatttgga
     8341 ttgattttta gcattacaga aaatctaaat aataaattta tcttttcatc ttttggaaaa
     8401 actggaatta ttgtaactgg agctatagga acgtttgttc atgaattgag tcatttgata
     8461 atgtgtttga ttttcatgca taaaataaat tcagttaagt ttttcagacc aatagaaagt
     8521 aaaaatgatg gtatactagg atatgtaagt catagctata aaaaagataa tctttatcaa
     8581 agtataggaa atttctttat aggaattgca cctattatag gtggaacaat aatgattatt
     8641 ttaattttta agatattact gccagatagt tatatgaaag tgactcaaaa tatagattta
     8701 aagttatatg taagtatgat taataattta aatataaatg ggtttataca cattatttta
     8761 ggtgatattt ccaattttat aagaataatg ttgttaacac ctagtgtata ttcattgaag
     8821 tattttatat ttatgttttt aatgtattct atatctacac atatgtcact tagtagtgct
     8881 gatttaaaaa atagcttaaa tggtctgagc tttatagtta tagttatttt tgtaataagt
     8941 tcaattacat acttatttgg atttgataac ttgaatatat ccagtttaat agttaagtat
     9001 aacatattta taagtttctt tatggcaata gggtttattt tttcaatagt aactctcata
     9061 atatcctata ttttaagctt agtcagccca tttaaaaact ttagttaaat aaatacatat
     9121 tttactaata tttggtttcg ttagaaacat ataaaaatat tatataaaaa agattttagt
     9181 agagcata
//
LOCUS       DACXMZ010000159         9089 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.160, whole genome shotgun sequence.
ACCESSION   DACXMZ010000159 DACXMZ010000000
VERSION     DACXMZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.160"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     repeat_region   472..698
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=538..567
                     /rpt_unit_seq="agttttagattaactatatggaatgtaaat"
     repeat_region   1301..1858
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1301..1329
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            2071..2259
                     /locus_tag="JD016_14175"
     CDS             2071..2259
                     /locus_tag="JD016_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000778.1"
                     /translation="MNKIATKSRAEYMKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNL
                     TKKEWLEEKIDEELEQKE"
     gene            2379..3200
                     /locus_tag="JD016_14180"
     CDS             2379..3200
                     /locus_tag="JD016_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HAU5000779.1"
                     /translation="MKNELMMFEEKKVEVLEYNGQVLFNPYDCGRCLELSDSAIRNHL
                     SKMNDTQAVLLKNSNVLDKDFRKLHNTGEKFLTESGVYKLIFKSKKEEAERFQDWISD
                     EVLPAIRQTGAYITNNADPDKLREKASEIEKLQLAYNSTSMLKELLDDAGFDNKSKLL
                     TAKTLYKKAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPAQLAVCEIIKKIKLEE
                     NEVKGVWETNGSWTGTVNKYTKSVIDKVRTWIEENNRPTKIAGEKKNFHVVYKIE"
     gene            complement(3654..3872)
                     /locus_tag="JD016_14185"
     CDS             complement(3654..3872)
                     /locus_tag="JD016_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5000780.1"
                     /translation="MNMSIKFFKLIDLLNRRGITKEELRLKIGASSTTIAKLSSNKNV
                     SLDVIDKICKALNCQPGDIMEYEDDEKL"
     gene            4144..4488
                     /locus_tag="JD016_14190"
                     /pseudo
     CDS             4144..4488
                     /locus_tag="JD016_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103644.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            4570..4923
                     /locus_tag="JD016_14195"
     CDS             4570..4923
                     /locus_tag="JD016_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000781.1"
                     /translation="MQFLVAKTFFKKAGVDIPLEIEEKEHFYDTKQIARKLGLYTMSN
                     KPAFQAVKQIIRKLDIKEDETKTVLETNGSWTGTVTKYSNKVTEKVKVWLKENNNPIS
                     IKGDKKNYYILYKNN"
     gene            5202..5460
                     /locus_tag="JD016_14200"
                     /pseudo
     CDS             5202..5460
                     /locus_tag="JD016_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5602..6384
                     /locus_tag="JD016_14205"
     CDS             5602..6384
                     /locus_tag="JD016_14205"
                     /inference="COORDINATES: protein motif:HMM:PF09851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HAU5000782.1"
                     /translation="MGLFGGKEPCSICGGKGKNKVLENEYLCNNCFINFTIFSRERLK
                     ATSAMQVLADHEGIRKFINFSKTNRELLAKFVETNRINKFVSIDEDNNFIKISDIRKG
                     GDVIENVYATDEIMQFELLEDGESIASGGLGRAVVGGALFGATGAIVGGITGKKTTRK
                     VVDTFKIKITFNNINNPLEYVNLINAKTKTNSSIYQNACNEAQEILSVLSIIIKNNDK
                     ADNEQSKTSDSIEQVKGLKELLDLGAITEEEFNTKKKELLNL"
     gene            6451..8802
                     /locus_tag="JD016_14210"
     CDS             6451..8802
                     /locus_tag="JD016_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAU5000783.1"
                     /translation="MATIQTSIRIFDGMTPAFRNMTNAINTTINSFDRLQQRLHNPLN
                     AGGIQISQQSLNNIENILTRIEQNIEKADEQQRRFNEDINKGVSNTDRLLGSVKKIAG
                     AYMGLKTIGGLGSLSDQMTSTNARLAMTNDGQLSDGGLNKMIFQSAERSRASYLDTAQ
                     IVSRIGMNAGSAFSSTREIVSFAEQLNKKFIIAGASTQEMSSALLQLTQGLGSGVLRG
                     EELNAVFESAPNIIRSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAAAEQTNTEFE
                     KMPLTIGQIFTSIKNNAIMIFGAIQKKIEDTVSSRGFRTFIVNITDAIYVLGNVGYSV
                     FNGLIDLFSSPAFQGFFNLMIVGVSMITQGLGWIITQALSVANVFAQNWSIISPVIYG
                     IVGAFIAYKVIIYSVLIAQTIYTAITFTQCFVTALLSGELYVATKAMLINKLMTLGVS
                     RANVTLCVTMIMVVATIALVIAAIFVGVAIFNHFAGASISATGVVVGAFYFLGACIYD
                     VFAGAWNIVMAFAEFFVNSFNIVIYNVQMLFYKFQNFVIDSMGDVGGSFDNCATALAN
                     AFVSAANIAIKGINGVIKALNLIPGVNINTLGSLDKIDSFVKQYKDYQKTLKEPIKPQ
                     DWKAPYAEIKNPFDSYKKGYEVGQNLENKLKDAFDINKIAEKAKKDLRLDDLWDKKYG
                     LGDGLGSAGLNSPLGDAAKGAKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQEVINKYT
                     GVNIKIDMNNTNNISKDTDLDGIVNVLTEKLNDAMVVSAEGVV"
     gene            8818..>9089
                     /locus_tag="JD016_14215"
     CDS             8818..>9089
                     /locus_tag="JD016_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAU5000784.1"
                     /translation="MAYDFYLDGVQLPITPGKLEIKTTNKNKTVDLINDGEVNILKTP
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIV"
BASE COUNT         3569 a          973 c         1666 g         2881 t
ORIGIN      
        1 ataataatcg aaagtgcgaa gggtgattat tttcatatta aacgccaaat tccaaataag
       61 gaaggaggtg aaattatatg ataggttttt tattaagcat actagctggt gttatatcag
      121 cttatattta tgacaaaata aaaaatcacc cagacgccaa taagggtgat ttaaaaaaat
      181 aatattttca cttaacaact gaaaataatc actctttgta ggagtaaatt atttccttgc
      241 ttttattata ccacaaattg gtacagatat tcaaaaataa tatttttatg atataataaa
      301 aatgtagaga ttttgcagtg agcgatattt gtgataaatt gaagtttaac agttgcaata
      361 taaggcgttg agggtgtgtg ataaatgtta tcaattgtac tactcatggt tcactgcaaa
      421 tttgagagag atgcgtatgt gtaggtattg gaaatgccaa gtttattttg gggttttaga
      481 ttaactatat ggaatgtaaa taccgcttta cctgctgtag tatttaaaga actaataagt
      541 tttagattaa ctatatggaa tgtaaatcct tatacacgac attataatat aagtacgttt
      601 ttagttttag attaactata tggaatgtaa attgtttatc ttttcattgt tcatgtacat
      661 ttcattgaag ttttagatta actatatgga cttaaaatta aaaatactga aaaacactta
      721 cttttatggt aggtgctttt ttatattaaa atctgtgtac ttaattgaaa tatttggtaa
      781 aatatgtaag aattgtatga tataataatt gtagcaagga aaataataat tgaaaagtgc
      841 taagagtggt tatttccata tttgaatacc aaattccata acggaaggag gtgtaacagt
      901 atggtgataa attttttatt gagtatactg gctggtgtta tatcagcctt catgtatgag
      961 aaaataaaaa accactcaaa ggccaataag agtggtttaa aaaaataatt ctttaaatca
     1021 attttgatgg aaatagctac tcttgtataa agtaaattat ttccttgctt ttattatacc
     1081 acaaattggt acagatattc aaaaataata tatttatgat ataataaaaa tgtagagatt
     1141 ttgcagtgtt cgatttttgt aataaaatat ggcttaacaa ttggaataca aggcattgag
     1201 ggtgtgtgat aaatgttatc aattgcacta ctcatggttc actgcaaatt taagagagat
     1261 gtgtatgtgt aggtattgga aatgctaagt ttattttggg gttttagatt aactatatgg
     1321 aatgtaaata taacatatga aggtatggaa cagacaagta aacaagtttt agattaacta
     1381 tatggaatgt aaatcgcatt tgtaagagtt gactttatat tttcccaagg gttttagatt
     1441 aactatatgg aatgtaaatt aaaaacgatt aaaccaaaaa acatcaatct gattaaatgt
     1501 tttagattaa ctatatggaa tgtaaatttt gttgttgtac aagggaatgt aactaaagat
     1561 gtaagtttta gattaactat atggaatgta aatttttcat caagtatatt tattgttaat
     1621 tggtctggaa agttttagat taactatatg gaatgtaaat ttcgcaactt atgatggtga
     1681 aatgattaca ttaacaggtt ttatattaac tatgtggtat gtaaagatta ctctaaaagt
     1741 ctttttatca ttttctacct caattttata ttaactatgt ggtatgtaaa taaagcaaaa
     1801 gataggaatg ataattttaa aactccagac ttttatatta acaatgtgga gaaaaactaa
     1861 atagaagaaa gaagcactta cttaaatggt aggtgctttt gttttgctca aaattggtcg
     1921 gttgagtaaa ataattagaa aaaaagatat agctgaggta gtgttttacg acgctagctt
     1981 caataaaatt ttatttgaaa aatattccaa aatagcttga ctgtaactcg ttacaatgtt
     2041 attattaatg taacgagtta cagaaaagag gtgaataaaa tagcaactaa aagtagagca
     2101 gagtatatga aaaatcgtcg aaaagataaa agaggtttta gtgtactttt agacaaagaa
     2161 aagttagata aatttgatga agtgttagaa gagaagaatc taactaagaa agaatggcta
     2221 gaagaaaaaa tcgacgagga actggaacaa aaggaataaa aaataagggt cactcccacc
     2281 gaccaaagtt tgagcaaccc ttattgacgt atactataca tcaactaact atagtatacg
     2341 tcattcctta aaaaatttca attaaggagt gtaatattat gaaaaatgaa ttaatgatgt
     2401 ttgaagaaaa gaaagttgaa gtacttgaat ataatgggca agttttattt aatccatatg
     2461 attgtggaag atgtttagag ttaagtgata gtgcaataag aaatcattta tctaaaatga
     2521 atgatactca agctgtatta ttaaaaaatt ctaatgtcct agataaggac tttagaaaat
     2581 tgcataacac aggtgaaaag ttcttaacag agagtggagt atataagtta atatttaaat
     2641 ctaaaaaaga agaagctgag agatttcaag attggataag tgatgaagta cttccagcca
     2701 ttcgacaaac tggtgcatac ataacaaata atgctgaccc agataagttg agagaaaaag
     2761 caagcgagat tgaaaaatta caattagctt ataacagtac atctatgtta aaagaattgc
     2821 tagatgatgc aggctttgac aacaaatcta aactattaac agctaagaca ttatataaaa
     2881 aggcaggcat tgatttacca attgagataa acgaagaaga acattacttt gacacaaaac
     2941 aaatagcatc taaactgaaa atatattcta agagtaataa accagcacag ttggctgttt
     3001 gtgagattat taaaaagata aaattagaag aaaatgaagt aaaaggagtt tgggaaacga
     3061 atggttcttg gactggtact gtaaataaat atacaaagag tgtaatagat aaggttagaa
     3121 cttggataga ggagaataat agacctacta agattgcagg tgagaagaag aatttccatg
     3181 ttgtatataa gattgagtaa atttgattgt attaaataat atattttagt ttattttagt
     3241 tttgaggggg attaatacaa tgcgtgaaag tttacttaat aaatataatt taaaaactga
     3301 tgaagatgta gaatactttg tgaagcttgc taatgcgtta tatgaactaa aactgaatag
     3361 tgaagaaaaa tttcaagaat atgcagagat attaagaggt attcttagag aacaagaaga
     3421 gagaaaataa gtaaaataat atagataaag cacttgaata ttacatagtt tcaagtgctt
     3481 tgtttgttaa aaaatggtat aatataggta ggaaattata ttaacaatgt ggtatgtaaa
     3541 gaagttagaa attaaagtaa caaataaaaa taaaactatt ttatattaac tatgtgggtt
     3601 caaaactaaa taaacaaaga agcacttact tttggtaggt gcttttgttt tgcttataac
     3661 ttttcatcat cttcatattc cataatatca cctggttggc agtttaaagc tttacatatt
     3721 ttatcaatta catcaagtga aacattttta tttgaagata gtttggctat tgtagtagaa
     3781 gaagctccta ttttgagtct tagttcttct ttagttatac ctcttctgtt tagtaagtca
     3841 attagtttaa aaaattttat agacatgtta atacctccta tatatttata taatactata
     3901 tatctttatt aaagtaaaga tatatttatc aaaataaata aaaagtattg acattattta
     3961 ctaaataatg tattattaga gtataaagat aaagaaatct ttataaaggt aaagtaataa
     4021 gggataaaat aaggaatatt ctctctcgtc aaagtttaaa tattccttat gacatataac
     4081 taatatgtaa tataattata tgtcattcct taaaaaaaat caattattaa ggagtgtgtt
     4141 tttatgaaca atttagtatt aattaacaac caagagttgc aggtcaagga gtttaataat
     4201 caaagagtag ttacatttaa agaaattgac caagtacatg aaagaacaga aggtacagca
     4261 aagagaaatt tctcagagaa tagaaaacat tttatagaga acgaagattt cttttttgta
     4321 aaagcaagtg attttgaaaa gtacggaaat cgtactttag aaattcctaa tcgtggatta
     4381 actttaataa ctgaaacagg ctatttgatg ttagtaaaat cttttacaga tgacttagca
     4441 tggaaagtac aaagacaatt agtaaatagt tattttagag ttaaagaaga aataaaaaca
     4501 ttagaagcag taaatgagtc aattaaatta ataactccaa tttttgaaga tttaaaaatt
     4561 gataaaagta tgcaatttct tgttgctaaa acatttttca aaaaagcagg agttgatatt
     4621 ccacttgaaa ttgaagaaaa agagcatttt tacgatacaa aacagattgc aagaaaatta
     4681 ggactttaca caatgtctaa taaaccagca tttcaagcag ttaaacaaat tataagaaaa
     4741 cttgatatta aagaagatga aacgaaaaca gtactagaaa ccaatggctc ttggactggt
     4801 actgtaacaa aatattctaa taaggttaca gaaaaagtca aagtgtggtt gaaagaaaat
     4861 aataatccta tctcaatcaa aggagacaag aaaaactact atatattgta taaaaataat
     4921 tagattttag tttagttttg agggggatta atacaatgta tgagaattta cttgatatgg
     4981 atagaataga acttatcaaa gaacttggaa gtatctttga aaaaatgaaa aatgaaaatc
     5041 cagataaatt ttatagattt gtaagtttag tgaaagaaga atgtaggaaa aaagaaaaga
     5101 aagagaataa ataatataga taaagcactt ggatattatg ttgtttcaag tgctttgtct
     5161 gttaaaaaat ggtataatgg atatagagaa ttatattaac aatgtggtat gtaaatacta
     5221 aattagaggt cgagaaggct attgaatcaa aaggttttat attaactaag tggtatgtaa
     5281 atcgatttta ttatagagta gaggaagggc ttgtaaatgg ttttatatta actaagtggt
     5341 atgtaaagta tatggacatt aaaaacccat ttgactctta taagttttat attaacaatg
     5401 tggtatgtaa attgttgtat ttgtggaagt gaaaagggac ttataccagt tttatattaa
     5461 ctatgtggac ttaaaattaa aaataatcaa aaacacttac ttgaatagta ggtgtttttt
     5521 taatgaaagg aggtgataat aatgtaaaaa ttttacttat atagtataat aatcttataa
     5581 aattatgtag ggggtaatat tatggggtta tttggaggta aagaaccatg cagcatatgt
     5641 ggaggaaagg gtaaaaataa agttttagaa aatgaatatt tatgtaataa ttgttttatt
     5701 aatttcacaa tattttctag agaaagatta aaagcaacta gtgcaatgca agtattagct
     5761 gaccatgaag ggataagaaa atttataaat ttttctaaaa caaacagaga actgttagca
     5821 aaatttgttg agacaaatag aattaataaa tttgtatcca tcgacgaaga taataacttt
     5881 attaaaatat cagatattcg caaaggtgga gatgtaatag aaaatgtata tgcaactgat
     5941 gaaattatgc aatttgaact tttggaagat ggagagtcta tagctagtgg aggattggga
     6001 agagctgtag ttggaggagc tttgtttggt gctactggag ctatagttgg tggaataact
     6061 ggcaaaaaaa caacaagaaa agttgttgat acatttaaaa ttaaaataac ctttaataat
     6121 atcaataatc ctttggaata tgttaatttg attaatgcaa aaacaaaaac taattcatct
     6181 atatatcaga atgcttgtaa tgaagcacaa gaaattttat cagtactttc aataataata
     6241 aaaaataatg ataaagctga taatgaacaa agtaaaacta gtgattcaat agaacaggtc
     6301 aaaggtttga aagagttgct agacttagga gcaataacag aagaagaatt taatactaag
     6361 aaaaaagaat tattgaattt gtaatattac aaagcactta cttaggtagg tgttttttta
     6421 tgtaaaaatt tgagaaagga gagtgaaaat atggctacaa tacaaacatc cattagaatt
     6481 tttgatggaa tgactcccgc atttcgtaat atgactaatg ctattaacac tacaattaac
     6541 agttttgata ggctgcaaca aaggttgcat aatccgctta acgcaggtgg aatacaaata
     6601 tctcagcaga gcttgaataa tattgaaaat atcttaacta gaatagaaca gaatatagaa
     6661 aaagcagatg aacaacagag aagatttaac gaagatatta acaagggggt aagcaataca
     6721 gatagattgc ttggaagtgt taaaaaaatt gctggagctt atatgggctt aaagacgata
     6781 ggaggattag gtagtttaag cgaccagatg accagcacga atgcgagatt agctatgaca
     6841 aatgatggtc aattatcaga tggaggatta aataaaatga tattccaatc tgctgaaagg
     6901 tctcgtgcat cttacttaga cacagcacag atagtctcaa ggataggtat gaatgcagga
     6961 agtgcatttt ctagtactag agaaattgta agttttgctg aacaactcaa taaaaagttc
     7021 ataattgcag gagctagtac ccaagaaatg agttcggcac ttttgcaact tacgcaggga
     7081 ttgggttctg gcgtattaag aggtgaggaa ctgaatgctg tatttgagtc agcacctaat
     7141 attatccgct cgattgccga ttatcttgat gtcgacatag gaaagattcg aggtatggcg
     7201 agtgaaggaa tgttaacagc agatattgta aaaaattcct tgcttgcagc agcagagcag
     7261 actaatacgg aatttgagaa gatgccactt acgatagggc agatatttac tagcataaaa
     7321 aacaatgcaa taatgatatt tggtgccata caaaagaaaa ttgaagatac agtatcaagt
     7381 aggggattcc gaaccttcat agttaatata actgatgcta tatatgttct tggaaatgtt
     7441 ggatatagtg tcttcaatgg gttaattgat ttatttagca gtccagcttt ccagggcttt
     7501 tttaatctaa tgattgttgg agttagtatg attacgcaag gactaggttg gataataaca
     7561 caagcactaa gtgttgctaa cgtatttgca cagaattggt caattatttc gcctgtaatc
     7621 tatggtattg taggagcttt tattgcttac aaagttataa tatattcagt tttaatagcc
     7681 caaacaattt acacagcaat tacttttaca cagtgttttg tgacagcgct tctcagtgga
     7741 gaactatatg ttgctacaaa agcaatgctt ataaacaagt taatgacttt aggagtaagc
     7801 agagctaatg tgacattatg tgtgactatg attatggttg tagctacaat agctctagta
     7861 atagcagcaa ttttcgtagg agtagcaata tttaatcatt tcgcaggagc tagtataagt
     7921 gcgactggag tagttgtagg agctttctat tttctaggag cttgtattta tgacgtattc
     7981 gcaggagctt ggaatattgt aatggcattt gcagagttct ttgttaattc gtttaatatt
     8041 gttatctata atgtacagat gttattttat aaattccaaa attttgtaat agattctatg
     8101 ggtgacgttg gaggaagttt tgacaattgt gctacagctt tagcaaatgc ttttgtaagt
     8161 gcagcaaaca tagcaataaa agggattaat ggagttataa aagctttaaa tctaatccca
     8221 ggtgttaata taaatacttt aggaagcttg gacaaaatag attcttttgt aaaacaatat
     8281 aaagattatc aaaaaacatt aaaagagcct attaagccac aggactggaa agctccatat
     8341 gcagaaatca aaaatccatt tgactcttac aaaaaagggt atgaagtagg tcaaaattta
     8401 gaaaataaat tgaaagatgc ttttgacatt aacaaaatag ccgaaaaagc aaaaaaagac
     8461 ttaagattag acgatttatg ggataaaaaa tacggacttg gtgatggatt gggttcagct
     8521 ggattaaact cacctttggg cgatgcagca aaaggagcaa aagataccgc gggaaatact
     8581 gcaaagatgg caaagactat ggataaaagt caagaggact taaaatactt aagagacata
     8641 gcagaacaag aagtaataaa caagtataca ggagtcaaca ttaaaattga catgaacaat
     8701 acaaacaaca taagtaaaga tactgactta gatggaatag ttaatgtatt aactgaaaaa
     8761 ttaaacgacg ccatggttgt atcagcagaa ggcgtagttt agaaaggagg tgtaatcgtg
     8821 gcttatgatt tttatttaga tggagtacaa ttaccaatta cacctggcaa attagaaata
     8881 aaaacaacaa ataaaaataa aactgtagac cttataaatg atggagaagt aaacatatta
     8941 aagactcctg gtctatctga aataagtttt gaagcagagt ttacacataa taaattacct
     9001 ttttgtagag gtcaatttag agatgttcaa ttctttttaa gtaagctaga attactaaaa
     9061 actgattgta agccatttca atttattgt
//
LOCUS       DACXMZ010000160         9015 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.161, whole genome shotgun sequence.
ACCESSION   DACXMZ010000160 DACXMZ010000000
VERSION     DACXMZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.161"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            331..642
                     /locus_tag="JD016_14220"
     CDS             331..642
                     /locus_tag="JD016_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000785.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            644..2569
                     /locus_tag="JD016_14225"
     CDS             644..2569
                     /locus_tag="JD016_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAU5000786.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            2572..3063
                     /locus_tag="JD016_14230"
     CDS             2572..3063
                     /locus_tag="JD016_14230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAU5000787.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            3115..3678
                     /locus_tag="JD016_14235"
     CDS             3115..3678
                     /locus_tag="JD016_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000788.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            3693..4670
                     /locus_tag="JD016_14240"
     CDS             3693..4670
                     /locus_tag="JD016_14240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HAU5000789.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            4663..4980
                     /locus_tag="JD016_14245"
     CDS             4663..4980
                     /locus_tag="JD016_14245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAU5000790.1"
                     /translation="MYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVEDEY
                     AIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGADIL
                     FKE"
     gene            5097..6875
                     /locus_tag="JD016_14250"
     CDS             5097..6875
                     /locus_tag="JD016_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase alpha chain"
                     /protein_id="HAU5000791.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            6872..8245
                     /locus_tag="JD016_14255"
     CDS             6872..8245
                     /locus_tag="JD016_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAU5000792.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            8251..8919
                     /locus_tag="JD016_14260"
     CDS             8251..8919
                     /locus_tag="JD016_14260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAU5000793.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
BASE COUNT         3311 a         1188 c         1813 g         2703 t
ORIGIN      
        1 aaataaaata aaaatttaaa atgtcgacat aacgttaatt ttaaaacaaa ataacgctat
       61 tgacaaacta tatcgcttta aattataatt attgaaaata atggtattta aaaaccatac
      121 tcatgtcata gtctaaaagt aagtcaaaaa aggatgtaaa tctaggcaag tactattcta
      181 aaatatgttt tattaagaaa taaacgttga aaaatattaa ttattatata aaaatgctgt
      241 ttatacttta acaatgaggt agtaatctaa ttagaataaa aaaatctaat tggttatata
      301 catagtaaaa aggtaagaga ggtgatttgg atggcttttg aaataataaa aagcattgtt
      361 gaggcagagc agacagcaga cagtatcaaa gtaaaagctg ttactgatgc agagtctatc
      421 agagctgatg ctgtaaacaa atgtgaaagc atatttgctg atgtaaaaaa acaagcaaag
      481 cttatggaag aaactcttat tgagaaggca gtcaccgaca gtagagcaga ggttgataaa
      541 atcttagcta atgctaaaag tgaatgtctg aaaattgaaa aaactgctga agaaagaaaa
      601 agtaaggcta ttgaagcggt tattggaaag gtagtgagat aatatggcaa tagttaatat
      661 gaacaaaata tctattgttg gcttagaagc tcaaaaaagc cagattttaa agctacttat
      721 gaaccgtggg tttgttcaga ttgatgatag tgcctttctt actgaggagg aggaattaaa
      781 aaattatctt gtaaaagata gtgaagaatc tgaagttata atgctagagc agaaaatgtc
      841 tcatgtaaac caagcaatta atattgtttc ttcactagtg aaacaaaaaa aatccctact
      901 tgcaccaaaa agagagttta acaaaattga tagtgaaaaa gaagggcaag tttatgctga
      961 agctctggag ttaaacaatt tgcacaagga gataggtaca ataaggtcta acataaactt
     1021 acttaacaac aataaaagta tgttagaacc atgggtgaac tttgatattc ctcttgaaaa
     1081 tatggaaact aagtatacaa aaactatact tggaactgtg cccataagcg caaacattga
     1141 tgctttaaga gtaaaattag aagatgaaat accagaaagt gttattggga ttgtcaattc
     1201 tgacaagtta atgaactatg tttatttaat tactttaaaa gaaacttttg atgatgtttt
     1261 agaagtttta aaagaattta atttttcagt ggtttcatta ccaaatgaag aaggtacacc
     1321 gttacaagct atcaagaaat acaatgaaat gataattaga caggaaaacg tttctaagga
     1381 caaaacagag gaaattaaaa gacatgcaga tagtatacac atgcttgaaa acctatatga
     1441 tttttacact atagaaagag accaaaaaaa gattatagag agacttgtca aaacaaaaac
     1501 aacattctgt cttaatggtt ggttaccaag caaaagggca gatgaattaa ttaaagaaat
     1561 tacagaaaag tttgattgtt gtgttcagac tgaagaagga agtaaagaag aaggcttccc
     1621 aattctgctt gaaaacaata aattagtaac accttttgaa agtgtaacta acatgtacag
     1681 ctatccaagt acaaaagata ttgacccaaa tacaatttta actttcttct ttgttgtatt
     1741 ctttggaatg atgctaagtg acgccgcata tgggttaata attgcagtag tttgtggatt
     1801 tgttgtatat aaattaaaga tacaaaaagg tgaaggaaat ttaattaaac ttattggtat
     1861 ctgtggagtt tcaactactg tatggggtct tatattcggg ggtattttgg gggatttaat
     1921 tcctataaaa gcattaatta atcctttaga ggatgttatg acacttatgg gaatgtcgtt
     1981 actgttcggt attatccata tatatgtagg tttgggaata aaagcatata cattaataag
     2041 aagtggaaag gtaatagatg caatctttga cataggtttt tggtatttgt gtataacagg
     2101 tgtatgtcta cttataatac cttttgttgc aggggacata ggtgtatttt cagaagtagg
     2161 aaaatattta gcaattatag gtgctatagg tcttgtactt actcaaggaa gaagctttaa
     2221 aggtattcct gttaagcttt ttaaaggatt ttcaagtctc tatggcataa caagttattt
     2281 tgctgatata ttatcttata caagaattat ggcgctttgt ttaacaacag gagttatagc
     2341 acaagttatc aaccttttag gagctatagc tggtccgata cttgcagttg taattggtgt
     2401 tgtaggacac acaataaacc ttcttataaa tgcactaggt gcatatgttc atacaagtag
     2461 gttacagtat gtagaatttt tcaataagtt ttatgaaggc ggaggagttc cttttgttcc
     2521 gtttaagtat aaaacaaaat atacaagcat taataaaaag gagatgtaaa tatggaattt
     2581 ttaaacgcgt taggaaatgg tcaatttttg gcaatatcag gagctgctat agcagcatta
     2641 tttgcaggta tgggttctgc taaaggtact tcaatagcag gtcaagccgc agcaggtgtt
     2701 gtaacagaag accctagtaa attcggtcaa cttctagttt tacaacttct tcctggtaca
     2761 caaggtctgt atggatttat catagctttc cttattttat caaaagcagg gattattggt
     2821 ggagatgcaa taccaacatc agctcaaggt ttacaacttc ttatggcagg acttccaatt
     2881 ggtttagttg gtttagtatc aggtatagca caaggtaaag cagcagcagc aggagttgga
     2941 atagttgcaa aaagacctga agaacttggt aaagctataa catttgcagc aatagttgaa
     3001 acatatgctc ttttaggact acttgtttct ttcttagctt acaatggaat tgcaattggt
     3061 taattataaa ttataattca taagtaaaaa aaatgaaaag gaggaagcac tcctatgggt
     3121 aatgaacaaa aaatgattga cagaattata gctgatgcca aacaagaagc acaagaaata
     3181 ttggataaag ctaaaagtga agcagattta aaagtaaatt cagctaatga aaaagctgaa
     3241 aaggaaatgg cttcctatac aaagcttgca gaggcagaag cagaaaaagc ggcttctaag
     3301 gaaatatcag gagcttatat ggaggctaaa aagcaaatat tatcaaaaaa acaagagatt
     3361 ttagaagagg taattttaga agctaaaaac aaacttttaa atcttaaaga taatgaatat
     3421 gaagagatta tactaaacat gattgaaaaa tcaaattgta cagatgattc agagatagta
     3481 ttatctaaaa aagataaaaa aacattaaaa gatgtgctat caaaaaaagg cattaaagtg
     3541 tctgatgaaa ctagagatat tactggaggt ttcatagtta aaaaaggtga cattgagtac
     3601 aattattcat ttgaagccat aattgcagta gagcatgaat atattgaaca aattgcagca
     3661 gaaattttgt ttaattaagg aggggttaaa aaatggcaga agaaaaaact tatccctatg
     3721 ccgttgcacg aataagagtg cttgaaaaac agcttcttaa cagacaaatg tttattcaaa
     3781 tggcagaagc caaaagccct gaagactctt taagaattat agctgaggca ggttatgctg
     3841 atacatcaaa taataatata cataattttg agaatatttt gactcaagaa ttatcaaaga
     3901 catatgaaat tcttaaaagt ctagctcctg aagaaaaatt tgttgatgta tttctttata
     3961 aaaatgacta tcacaattta aaagtgctga ttaaagaaga aatttctggt gtagatggtg
     4021 agaaatattt aattgatgga ggtacaattc ctcttattaa gcttagagaa tctttagcaa
     4081 atcgtagttt tagtgattta cctaagataa tgtcatcagc tatattagag gcatttgatg
     4141 tttataataa gacacaaaat ggccagatgg ttgatattgt tctagataaa gcaaccttta
     4201 ccagtatgaa agaaactgct aaagaaagcc aaaacaaatt tgtaatagat tatgttatga
     4261 aagtttgtga tttaacaaat ctaaagagtt ttataagagt caaaaatatg aagaaagatt
     4321 ttgatatgtt tatgaatgtg tttgtttcag gtggctcact tgataaggaa aaattcttag
     4381 aagctttttc ctcagatact ccagcatcat gttttaaatc tacttcttac tctgatgtct
     4441 gtaaaaatgg catggatagt ggttttactg tctttgaaaa actttgtgat gactatctta
     4501 tggaatatgt tagaggagca aaatttaagc ctcttacatt agaaccacta attgcatatt
     4561 tatatgctaa agaatcagag ataaaaacaa ttagaattat tttgactagt aagcttaata
     4621 atatagacgc agatactata aaagaaaggc taagggatgc ttatgtataa ggtaggagta
     4681 gttggagata aagatagcat catgggattt ttagctcttg gaatagatat cttcccagcc
     4741 tatgatagtg acgaaattaa aaaaagtata cataagttgg ttgaagatga atatgcgata
     4801 atttatatta cagaacaggc atcattgctt gcaaaagaat caatagcaaa atacaaagat
     4861 taccaacttc ctgccattat agttattcca ggtattggag gaagtatggg attaggtatg
     4921 aatgaagtaa gagaatctag taaacgtgct ataggtgcag atatattatt taaagaatag
     4981 atgaaagttg aataaaagac aatctcaaat tttattggtg ctaaatatat gtactaataa
     5041 aatataggag ggaatattta aattttgata aagtatatga ttgcaggtga gagaatatga
     5101 aaacaggcac aattgtaaag gtgtcaggac cgcttgttgt agcggagggc atgagagatg
     5161 cgaatatgtt tgacgtcgta agagtttcag ataaacatct tataggcgag attatagaaa
     5221 tgcatggaga taaagcttct atacaggttt atgaggaaac ttccggtcta ggaccagggg
     5281 aagaagtagt ttctcttgga atgcctatga gtgttgaact tggaccaggt cttatttcaa
     5341 ctatttatga tggtattcaa cgtccacttg aaaaaatgta tgatatttca ggtactaata
     5401 taactaaagg tgttgaagta tcttcacttg atagaacaac taagtgggaa tttaaaccta
     5461 aaaaatctgt tggtgataag gttgtagcag gagatataat tggtggcgtt caagaaacag
     5521 cagtagtaga atgtaaaatt atggttcctt atggagttaa gggtactata aaagaaatat
     5581 attctggtga atttactgtt gaagatacag tttgtgttat tacagatgaa aaaggtaatg
     5641 atatacctgt aactatgatg caaaaatggc cagttagaaa agaaagacca tatagagaaa
     5701 agaaaacacc agattcactt cttgttacag gtcaaagagt tatagataca tttttcccaa
     5761 tcacaaaagg tggggtagca gctataccgg gaccttttgg aagtggaaaa actgttacac
     5821 agcatcagct tgctaaatgg gcagatgcag atatagttgt atatataggc tgtggcgagc
     5881 gtggaaatga aatgacagat gttcttcttg aatttcctga attaaaagac ccaagaacag
     5941 gcgagtcact tatgcaaaga actgtgctta tagcaaatac atcagatatg ccggttgctg
     6001 cacgtgaagc ttctatatac actggtatta caatagctga atattttaga gatatgggat
     6061 atagtgttgc acttatggca gactctacat caagatgggc tgaggctctt agagagatga
     6121 gtggtcgttt agaggagatg cctggtgaag aaggttatcc tgcatactta ggttcacgtc
     6181 ttgctcaatt ctatgagaga gcaggaaagg taaattgtct aggtatggat gaaagagaag
     6241 gaacacttac agcaattggt gcagtttctc ctcctggtgg tgatacatca gaacctgtca
     6301 gccaagcaac gcttcgtata gtaaaagtat tctggggtct tgatgcatct ttagcatata
     6361 aacgtcattt cccagcaatt aactggttga ctagttattc actctatcag gaaaaagttg
     6421 atgtatgggc agataaaaat gtaaatgata atttctcagc tcaaagagct caagctatga
     6481 aacttttaca agttgaatct gaacttcaag aaatagttca attggttggt gttgattcac
     6541 tttcagatgg agatagattg atactagagg ttactcgttc tataagagaa gacttccttc
     6601 atcaaaactc tttccatgaa gttgatactt atacatcact tcataaacaa tatagaatga
     6661 tgggacttat attaaaattc tataagtcag cacaagatgc tatcaagcaa aatgttacta
     6721 taaacgatat tatcaagctt tcagttcttg aaaagattgg tcgtgctaag tatgtagaag
     6781 aaggcgatat agatgcagct tacgattcaa ttgaagatga aatagacaac gagcttgcag
     6841 accttattaa aaagaggggg gctgaagaac tatgataaag gaatataagt caatacaaga
     6901 ggtagccggt cctcttatgt taataaacgg cgtagaaggt gtaaaatttg atgaacttgg
     6961 agaaattgaa ctttcaaatg gagaaattcg acgttgcaaa gttctagagg taaatggaga
     7021 tactgcactt gttcagcttt tcgaaagctc aacaggtata aaccttgctg aaagtaaagt
     7081 tcgtttttta ggaaagagtc ttgactttgg agtttcacca gatatattag gtcgtgtttt
     7141 tagtggaatg ggtagaccta ttgatggagg tccagaaatt ataccagata aaagacttga
     7201 tataaatgga gctccaataa atccagcagc acgtgattat ccggccgagt ttatacaaac
     7261 tggagtctct gctatagatg gacttaatac attggttaga gggcaaaaac ttcctatatt
     7321 ctcagggtct ggtttgccac atgccaatct tgcagttcag attgcaagac aggcaaaagt
     7381 acgtggaact gattctaagt tcgccgttgt atttgctgca gttggtataa cttttgaaga
     7441 tgctgaattt tttataagcg attttaaagc aactggtgca atcgaccgtt ctgtagtgtt
     7501 tgtaaacctt gcaaatgacc cagctgtaga gcgtgtatct acacctagaa tggcacttac
     7561 agcagcagaa taccttgctt tcgagcaaga tatgcatgta cttgttataa taacggatat
     7621 tacaaactat gcagaggcat tgcgtgaagt ttcagcagct aaaaaagaag ttccaggacg
     7681 tcgtggatat ccaggatatc tttatactga ccttgctact atgtatgaaa gagcaggaaa
     7741 aatgaaaggg catgaagggt ctataactat gattccaata cttacaatgc ctgaggatga
     7801 taagacacat ccaattcctg accttacagg atacattaca gaagggcaaa ttatacttag
     7861 ccgtgaactt tataaaaaag atatacagcc tcctatagat gttttaccat cactttctcg
     7921 tctaaaagat aaaggtattg gtaaaggtaa gactcgtgaa gaccatgcag atacaatgaa
     7981 ccagcttttt gcagcttatt caagaggtaa ggatgcaaaa gaacttatgg ctatacttgg
     8041 tgaatctgca ctttctgaga ttgatttaat gtatgctaag tttgcagatg aatttgagaa
     8101 agaatatgtt tcacaaggat ttagaactga tagaactatt gagcagacat tagaaatcgg
     8161 atggaagctt cttagtatgt taccaaaaag tgaacttaaa cgtatccgtg atgaatattt
     8221 tgaaaaatat atgcctaagg agtgatatct gtggctagat taaatataaa tccaacacgt
     8281 atggaaatga ctagacttaa gaaactcctt aaaactgcta caagaggtca taagcttctt
     8341 aaagataaac ttgatgaact tatgaaacaa tttcttgaga ttgtaagaga aaataagcga
     8401 ttgcgtgaag aagcagaaaa tgctcttgat actgcttata aaaactttat aatcgcaaga
     8461 gcagttatga gtcaggagta tctgggttca gcacttatga tgccaaagca atcagtttca
     8521 gtagatgttt ctacgagaaa tataatgagt gttgatgttc ctgtgtttga ctttaaaact
     8581 gaaaacaatc aaagtgatat ttatccttat ggtcttgctt ttacatcagg tgaattagat
     8641 tctgctatgg aagctttctc agatgctatg caaccacttt taagacttgc agaaagtgaa
     8701 aaaagtgcac agcttcttgc acaagagata gaaaagacac gtagacgtgt taatgctctt
     8761 gaaaatgtaa tgataccaaa ttacattgag actattaagt atatagctat gaagcttgaa
     8821 gaaaatgaga gagcaagtac tactagactt atgaaggtta aggatatggt gcttaaaaaa
     8881 gcacttgaag aaaagaaaaa aaatgatttg gttgtataat taaatactta taaaaataag
     8941 tctaacattc tactttatag aggttagact tattttttat gtttaaatta aaatgtagat
     9001 actataaaaa ttaat
//
LOCUS       DACXMZ010000161         8891 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.162, whole genome shotgun sequence.
ACCESSION   DACXMZ010000161 DACXMZ010000000
VERSION     DACXMZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.162"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            74..415
                     /locus_tag="JD016_14265"
     CDS             74..415
                     /locus_tag="JD016_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HAU5000794.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            566..979
                     /locus_tag="JD016_14270"
     CDS             566..979
                     /locus_tag="JD016_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HAU5000795.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1181..1822
                     /locus_tag="JD016_14275"
     CDS             1181..1822
                     /locus_tag="JD016_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAU5000796.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1846..3099
                     /locus_tag="JD016_14280"
     CDS             1846..3099
                     /locus_tag="JD016_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAU5000797.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3145..3795
                     /locus_tag="JD016_14285"
     CDS             3145..3795
                     /locus_tag="JD016_14285"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAU5000798.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNAKEVIQIAENSDIDFKDNNIYLDEEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3846..4442
                     /locus_tag="JD016_14290"
     CDS             3846..4442
                     /locus_tag="JD016_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAU5000799.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4455..5294
                     /locus_tag="JD016_14295"
     CDS             4455..5294
                     /locus_tag="JD016_14295"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAU5000800.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5539..6693
                     /locus_tag="JD016_14300"
     CDS             5539..6693
                     /locus_tag="JD016_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000801.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            6999..8696
                     /gene="ade"
                     /locus_tag="JD016_14305"
     CDS             6999..8696
                     /gene="ade"
                     /locus_tag="JD016_14305"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009109554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAU5000802.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKLYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3638 a          854 c         1576 g         2823 t
ORIGIN      
        1 catatatttc cgatagatta tattgataaa aaacactaaa attaaattaa taaaagtact
       61 tggagggatg gaaatgaaag aggaaaattt tattaatgtt gaatacgagg ataaacgttt
      121 ttcaattaat agtaaagaag ttttgtacat agaaaagcaa gagtatgaag taaagattta
      181 tactgaaagt ggtaattatg aagttggaga aaatgctcct tgtataagaa aactattgaa
      241 agaagaaaat tttttcaaat gcagtaatag ttatattgta aatctagaca agtgtgaatt
      301 ttatctcagc aataattgta tttttgtgaa taataatttt ataagaattt taggttcaga
      361 aagaaaaaag aaattaaaaa agaaattaga aaaattatat gattatgaag attaatattg
      421 agaggctgta ttttatacag cctctttatt tattgattgt attattaata aattttaaat
      481 tatgataaaa tataaaacaa gtaacttatt tcagaataaa atacttttta aaagaaaggg
      541 acgagatatg ggagattata ataaaatgat tgaattaaag aataacttag gtatcagtga
      601 aagaaagtta aaatatcctt gtatctataa acattttaaa gggaaatatt atgctacaat
      661 gggtcttagc aaggctattg atgatataga aaacatatgt gaaatatatg gtaaagaaaa
      721 tttaatccaa aacagaaata aatataaatt agttattaga catactgaaa gagaagaaga
      781 tatctatgtt tatagggatt tagatggaaa cttttatcat aaaaaagaag aagatactaa
      841 tgatttagtt ttatacaaga ccttatatga tgatacagga atatttgcaa gacctttgga
      901 tatgttttta gaaaaagtag atacagataa gtatgtcaat tcaattcaag aatacagatt
      961 tgaagaagta tataaataat ataagagaaa tatgtttata aatttagaaa aattctttaa
     1021 cagtacaata atttgcagaa atacaaatga gatttattgt aaataatatg ttagattaga
     1081 ttataaaata gaaacaaagt aaagtattat atgatataat taagttttga gaagtgataa
     1141 tttatattta ataaaaaata ggcatatatt aggaggacat atgggaaata ctttttatat
     1201 agtaagacat ggacaaacgg attggaatat attgggaaaa acacaaggtc atggtaattc
     1261 tgaccttacg cctcaaggta tagaacaggc aaaagaactt tctgaagata ttggcaagta
     1321 ttcaattgat tatattttta gtagcgacct tgggagagca atgcaaacag ctcaaatttt
     1381 aggagataag ctcaatattg aagttcaaaa aactgaggca cttagagaaa tgggattcgg
     1441 agtttgggaa ggccttttaa taaaagaaat tcaaaaagat tattcagata tatatgcaac
     1501 atggagaaat gaaccacact tagtaaatat accagaaggc gaaactctta agattataaa
     1561 ggaaagagta gatgctttta taaaggaact aaatgaaaaa tatgataata aaaatataat
     1621 tcttgttaca cattcaatta cattgagggt tatgttgttg tcatttttag aatcaggtat
     1681 ggaaaatata tatagaatta agcaagataa tactgcttta aatatagtag aatttaaaga
     1741 ttatggacct gttatagtaa aaatgaatga tacaagtcat ataaaaaatc atgtaaagat
     1801 aaataattct gcactggagt aggaatcaat tataaggaga aatttatgag taaagttata
     1861 gtagttggtg gaggagcttc tggaatgatg gcagctttgt cagcttcaaa aaacaataat
     1921 gaagtaatat tagttgagag aaatggagaa ttaggaagaa aacttagggc aactggtggc
     1981 ggtagatgca attttaccaa taatagagag atagaagatt tttttgataa agttgtcagt
     2041 aataaaaaat ttctctatag tagtttctat acatttacaa ataaagacct tatttcgtat
     2101 tttgaaagta gaaatttgga atataagatt gaagaagaaa atgaccacaa agtatacact
     2161 aaaaatgata aatctataga agtaatagaa gtattaaata aagatttact caatcacaat
     2221 gtaaagataa tgtataataa gaaagttata gatattataa ctgaagaaat tgctctaaaa
     2281 gatgattcta ataaggacaa gtcaaagtat ctaataaaag ggattatttt agataatgga
     2341 gataagatac taggagacaa ggttattata tcaactggag gagtgagtta ttcaaagacg
     2401 ggttcagatg gttcaatgta taagattctt aaaaagcatg gacatacatt aaacaaacta
     2461 tatcctgctt tagttccact tacaatagag gaaaaatgga ttaaagattt acaaggaata
     2521 tccatgaaga atgtggaaat atcttgtaag attaagaaaa ggaaaatatc aaaatctgga
     2581 gacatgctat ttgcccattt tgggataaca gggccttgtg ttcttataat gtcttcttat
     2641 attaacaaga ttattgaaaa ggaaaaggta gaattaaata tagatttctt accaaattta
     2701 agtactgatg aaatctctag tataatcaga gcatttccaa ataaaaatgt tctaaataat
     2761 cttaagcaaa tactacctca aaatttctta aaagagattt tttctctatt atctttagtt
     2821 gataaaaagg caagtgattt gtctaaggca gatgaaatta gaattattga gtatataaaa
     2881 aatatgaagc taacttgtaa tgggactaca ggtataaaca ctggtatggt tacttctggt
     2941 ggtatttcag ttaaagaaat taattcttct actatggaat ctaaattagt taagaactta
     3001 ttttttacag gtgaagtaat tgatatagat gcagaaactg gtggatataa tttacaaata
     3061 gcattttcaa ctggatatct tgcgggtata agtgtataaa tataattttt taataattaa
     3121 atatgaataa atagaggtgt atatatgggt aatttagtta tagctgtaga tggtccagca
     3181 ggagctggaa aaagtacaat agccaagata gtagcaaaaa agctaaatat aaattatata
     3241 gatacaggtg ctatgtacag agcagttact tataagtgtt taaaaagtgg tattgatgtc
     3301 aataatgcaa aagaagttat tcaaatagct gaaaattcgg atatagattt taaagataac
     3361 aatatatatt tggacgaaga agttataaat gaagaaataa gaacaataga agtaagcaat
     3421 aatgtttcta atgtagcaaa aataaaagaa gtaagacagt tgatggttga agttcaacga
     3481 aaaataggaa tgaaaagttc agtaatacta gatggtagag atataggttc atatgttttc
     3541 ccagatgcag attacaaatt ctttttagta gctactccag aagaaagagg aaatagacgc
     3601 tataaagaac tttgtaataa aggatataat actactttag aagaagtaat tgaggatata
     3661 ataagacgag atgaaattga ttcaaataga gaatttgcac cattggtaaa agctaatgat
     3721 gcactagaaa tagatactac aggaaaaact atagaagaag ttgtagaaga agttgtatct
     3781 aaaataaatc tttagttttt tatgcatcat aatgaaaata aatgtagtgt tgaagggaga
     3841 taaacgtgaa tttttataga tttgtaataa atatttttaa gggtttttct aaaatatttt
     3901 ttaaatatga agttataggt gcagaaaata ttccagatag aggaaatata gtaatagcat
     3961 caaatcataa atcgaatctt gaccctatat tcttggctgc tgccatagaa aatagagaaa
     4021 tagctgcaat tgcaaagaaa gaattgttta aagtaaaacc cttgggattt atattaaaaa
     4081 aattgcatgt tatgcctata aatagagaaa aaccagatgt atctacaata aaaactattt
     4141 taaggtcagt tagagatggt tatgtattag gaatatttcc agagggtact agaataaaag
     4201 gagattcttt tggtaaggct aaggcaggtc tttctgtatt tacaataaaa agtaaatcta
     4261 aagttgttcc agtatcaata atatctaaat ataaactttt tagtaaagta attgtatata
     4321 ttggagaacc aatatcattt gaagaacatt ttaaggaaaa attgagtaat gatgaccatg
     4381 aaaggatatc acaagagatt ttagaagtga taaaacaaaa ttactttaaa tattcaaaat
     4441 aagggggtaa agttatgaac ataaaaatag caaaaaatgc tggcttttgt tttggagtaa
     4501 aaagagcaat gaaaatggca tgggatgaag ttgaaaaaaa tgattctggc atatatgctt
     4561 taggtcctct tattcataac aagcaagcag tagcaaaata tgaagaaaaa ggtcttaaga
     4621 ctgtgaatga aattgataca ataccaaatc atgaaaatat gataataagg tctcatggag
     4681 ttcctgaaaa tatatataag gaagccaaag ataaaaaatt aaaaattgtg gatactacat
     4741 gtccatttgt aaagaaaata catacagtag taagtgaata tcataataaa ggctatgaaa
     4801 taatagttat tggagatatg aagcatccag aagttatagg tataaatgga tggtgtgaaa
     4861 attcagctat aataataaag accttagaac aaatggaaaa tatggaattt gataactcca
     4921 aaaaatattg tcttgttgca cagactacaa taaatccaga gttgtatata agtatagtta
     4981 ataaactatc agataaatta gaagaaatag tatttaatga tacaatttgt tcagctacaa
     5041 aaacaagaca agaatcagct aaagagttag ctaaagaagt tgattgtatg atagttatag
     5101 gaggtaaaca cagttcaaat actcaaaagt tagtaaaggt ttgtgaagac ttagtaccaa
     5161 cttttgcaat agagactaaa gatgaattgg atgtaaatac gcttaaaaaa tataaaaatt
     5221 tgggtattac agcaggggca tctacaccta attggataat agaagaagtt gtaacattcc
     5281 ttgaaaattt ataatttgat aggtaataaa aataggtgga cattgttata atttattata
     5341 atatccacct ttgatttcat attacatgtg gtaaatagat aacctaatat caaaaagtgt
     5401 gtatcttaag atgaaaaact aagagtataa gatgcgcact ttttaatatt agaaattcaa
     5461 aagaaatatt gatattgatt atcaatagaa aatagaatat aatataattg ttgcaaaatt
     5521 agtctaggag gtaaattaat ggaaatgttg atatccgctt ctgaagaggc gtttttacat
     5581 gtaggttcta tgattggatt ttttattttg ttatttggat atattaatta taaaactagt
     5641 ggaaatttta ctaatatcat atcaaaaaac agaaaatttc aacccttaat aggtgcttta
     5701 attggagcta taccaggttg tggcggaagt ttggcaataa tgccattgta tataaatgga
     5761 aaattaagct ttggagcaat aattgcaagt ttaatagcta gtatgggaga tgcagcattt
     5821 gtacttattt catcaaatat taaaatgtat ttttttgtca ctatagttag tacaattaca
     5881 ggaataataa ctggacaatt agtggattac tttaaactag aagaaaaatt agggttaaaa
     5941 aaccgtagaa aatcagataa atatgatata agtaaaaata accctaaaga aaataaagaa
     6001 caacataatc atgatgaaat aattttagac actctagcta aatctcatgg aaatacaaat
     6061 agacttgcat ttataataac acatggaaag ggatataaaa tttatatagg aattatagta
     6121 atcggtttta tatttatgtc acttgctcac tctggactta atcttcctat aattgagaag
     6181 ttacattcat tggaagaagt aatagcagtg attggaatat tattttcaat aatttatatg
     6241 tggtgtttta aaaaggtatt taaaaattct aatcaaaaag aggaagaaaa taaaaagatt
     6301 tctttgcgag aaatgcttat ccattctgtt ggagaaatat catttgtaat tacatggatt
     6361 tttattgcat atcttattta cgatttgata atattaattt taggtggaga tgaatactta
     6421 gttaaattag tattatcaac aggtgtaata agtgtattta taggtgcagg tcttggactt
     6481 attccaggtt gtgggataca aattgtgctt atgtcatttt atttgaaggg aactatacca
     6541 cttggtgcag tgatagccaa ctctatttct caagatggag atgcactttt cccactttta
     6601 gcaatggata aaaaatcttc tttatgggct atgattatta ctactatacc agcaatactt
     6661 gtaggtatga tagtttatgc atttttaggt taaatataat tatgaataaa gaagtattaa
     6721 aaaaataata aagcacataa atttatttat aagaatagtg aagattaaat ataaaaataa
     6781 aaaattcaat atgaattaaa ttattgctta aatattaatg agaacatggg agataatatt
     6841 attcctcatg ttctctctat ttaattaaag gaattatgga acaaatttca agtgatactt
     6901 tctatcaaaa ctcatatata atatatatta ataaattaaa ctataatacg taatacagtc
     6961 agtatattga tataaaaaac ttaggaggag ttgtaaaaat gggtaattta aaaagacagt
     7021 gtgatgtttc aagtggaaga gaaaaagctg acattgtact aaaaaatggg acaatcataa
     7081 atgtatttac agaagagtta ataacaggag atgtagcaat agtaggagac actattgttg
     7141 gaattggcga ttacaagggc aatgtagaaa ttgattgtag taacaaatat atatcacctg
     7201 gctttattga tgcacatatg catattgaat ctacaatggt aatgccaatt gaattatcga
     7261 aaaaattatt aaaatcaggt acgacaacta tcatagccga cccacatgag ttggtcaatg
     7321 taaaaggagc tagtgcaata gattttttat tagaatcgac aaaagatata ccacttaatg
     7381 tatatattat ggtgccttca tccgtacctg caacaagttt tgaaactaat ggagttggta
     7441 aatttagtgc aaaagacatg gagagttatg taaataatcc tagaatttta ggtttaggag
     7501 aggtaatgtg ttttaatgat gttataaatt ctgaaaatga aatcttggat aaactagagc
     7561 tttttaaaaa taaagttgta gatggacatg cacctaatat aaatggtaaa tcacttcaga
     7621 catatgtatg tgcaggaata gaaaatgacc atgaatgtat aacttttgat gaagtatatg
     7681 aaaaattgag agcaggatta aaaattttaa taagagaagg aagcgcagca aaaaatttaa
     7741 agtctatagt aagtggtatg ctaaagcata acttgccaat tgaagaattt atgttttgca
     7801 cagatgataa gcacttagat gatatagaaa aacaaggtca cataagatgg aatataaaat
     7861 gtgcgattga tttaggtatg gaacctgtta gggctataaa agttgcaact tataattctg
     7921 caaaagcata tgggttaagg aaaattggag caataggtgc gggttataag gcagatattg
     7981 ttgtattgaa tgatttagac aaaatggaag tggatagtgt atataaagat ggtaatttgg
     8041 taaacgaaga gatgttttct aattacaatt atgagattaa agataaagaa ttgcttaata
     8101 cagtcaagtt taaatatatt aataaagaaa aaatacagtt aaaagtaagt gaaaaaaatt
     8161 atgtaatgga gattgtacct tatcaaattt tgacaaataa gttatatgaa tctttaccat
     8221 gtgcagatgg atattttgtt ccaaataaag agtattcaaa actttgtgtt gttgagagac
     8281 atagaatgac tggaaatgta gcaattgctc cactaaaagg atttggcata aaaaatggag
     8341 caattgctac tactgtggcc catgattctc ataatattat agttgcaggg gataatgatg
     8401 atgatattct agtagctata aattacttaa aggaaataca aggtggttat gtaattgtat
     8461 caaatggaaa agttcttgca cacctaagtc ttcaagtagc tggattaatt agtactttta
     8521 cagctgaaga agtacaagaa attactgata atatgttgga gatagcaaga aaaatgggag
     8581 ttccagagta tgtagaccct tttattacac tttcttttat ggcacttcca gttgttccac
     8641 aaataagact tacagattta ggtctatttg atgttgagga atttaaattt atatagataa
     8701 tataaaagta ataataaatg aggctttctc aaaataggga taagtctctt tttattgttg
     8761 aaatgatgaa aatatatttg atttcataca aaaaagagca tctcatgaac taaaaatttt
     8821 atttttagat atccttattt taattaatct atcatttaac cttgttttaa gcatagatat
     8881 cttaataaaa c
//
LOCUS       DACXMZ010000162         8863 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.163, whole genome shotgun sequence.
ACCESSION   DACXMZ010000162 DACXMZ010000000
VERSION     DACXMZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.163"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(27..635)
                     /locus_tag="JD016_14310"
     CDS             complement(27..635)
                     /locus_tag="JD016_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAU5000803.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(744..3491)
                     /locus_tag="JD016_14315"
     CDS             complement(744..3491)
                     /locus_tag="JD016_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HAU5000804.1"
                     /translation="MGNMESKGTLSKNKVYILANGLFLAIALLNINLYTVSINMYRII
                     LLWIFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIIN
                     MSTFIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFN
                     LLYLLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNH
                     TYYVYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQR
                     KELLRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEK
                     LKKFDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKD
                     YENIILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNI
                     DFFDNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNK
                     LFKLLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFREL
                     FGISSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFG
                     QKRSRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQ
                     LIENLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFD
                     IIDITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLE
                     GGEILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCL
                     TKSLIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIY
                     K"
     gene            complement(3630..4421)
                     /locus_tag="JD016_14320"
     CDS             complement(3630..4421)
                     /locus_tag="JD016_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAU5000805.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4457..5113)
                     /locus_tag="JD016_14325"
     CDS             complement(4457..5113)
                     /locus_tag="JD016_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000806.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5106..6071)
                     /locus_tag="JD016_14330"
     CDS             complement(5106..6071)
                     /locus_tag="JD016_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HAU5000807.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6177..6283)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6573..7517)
                     /locus_tag="JD016_14335"
     CDS             complement(6573..7517)
                     /locus_tag="JD016_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HAU5000808.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNESTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKCCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            7628..8095
                     /locus_tag="JD016_14340"
     CDS             7628..8095
                     /locus_tag="JD016_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000809.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8265..>8863)
                     /locus_tag="JD016_14345"
     CDS             complement(8265..>8863)
                     /locus_tag="JD016_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000810.1"
                     /translation="KLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNNE
                     GYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRDK
                     IQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQND
                     FIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         2865 a         1466 c          871 g         3661 t
ORIGIN      
        1 gatacatatc ttacctatat aaaaacttat tctatataaa aacgccataa aaaatctttt
       61 gcttcttctg cataatctat atttatacgt ttactagttt ttatatctaa ctcatcttta
      121 ctaaatactt tttttccttt atcatcataa taaaaatcac ttatatataa ttcttcaccc
      181 attatactct tactatttag acttctatct atttttaaag ctttgcaaag ttttcctggt
      241 ccgttagtaa tattttttac ttgatattta ctcaactcag tgtaagcttt gttgtatcta
      301 ttcatagata tctcatctaa agaagttata ggctctaccc ctcttattaa tacacactct
      361 ggcacatttt ctatatttgc agataaattt aagcaattat acataccata aattaaataa
      421 acatatatat gtccaccatc taaatataat ggcttagttc tatctgtctt tttattgcca
      481 tatacatgtg ctcctttatc atttacaccc atgtatgctt ctgtctctat gattttggta
      541 acaattactt tattttcata tttccttatt agatatttac ctaatattga tttggctaaa
      601 tcaattccat tttttctaaa aaaatccttt tccaaaattt cacctcataa aatttattta
      661 aaacgcatca aatacaacta aagtaataaa taaaattaac tcttattaat gtgatatata
      721 taaacaataa atttagacat ttattattta tatatatcaa aaaattctat ctcaaacttt
      781 tcaatattat cattgtaatt tgaattatca tatacttctt caacaacctc tttaattggg
      841 aatataactt ttacattagt tccttctcct accttactat ctatgtaaat cttcccattt
      901 tgaatttcaa tcaatgattt tgtaaggcat aaacctatac cacttccaaa ttcatttgat
      961 ataccactgt tttcaatttg tttaaatcga ttaaatatat catttaattt ttctttgtct
     1021 atgcccactc cagtatcttt tataattatc tctactgttt cgtctttttt atatatacct
     1081 acaaggattt ctcctccttc taaagtaaat tttatagcat ttgataatat atttagtatt
     1141 attctttcta tcgattctgg gtcaagccct accataacag tttcttcatc tgtatcaaaa
     1201 attaaatcta ttcctttaga cttagcataa ctactaatag atgtaactat tctttctgtt
     1261 atgtcaataa tatcaaaatt aactgcttta atcttatata tgtctgactt aacttgagaa
     1321 atatttatta tattatctat taatctcaat aatctaaaca tattttgttt agttattttt
     1381 atatgttctt ttagtatatc ctgaaaatca attatttttc cttttttata aaggttttca
     1441 ataagttgaa ctgaactata tatattattt aaaggtgtct taagttcatg agatatatta
     1501 gcataaaact ccatcttcat ttggtcatac tgtctttgtt ttattaatgc ttttttaagt
     1561 ctttcagact ctctttgcac acttaaatct ttaataattc tcactctact tcttttctgt
     1621 ccaaagtata atgttcttga tatcattttt acttctttaa tgtgattatt ttcatttact
     1681 atatggatac tcttctctct ttctcctata tttaaattgt ttatgtattt ttttactaat
     1741 atatttctat atttttgtgt tgtaactcct aatacatcac tagaaattcc gaacaactct
     1801 ctaaaggctc tatttgtata tagtactctt gatgtactta aatcctcaag aaaaatagcc
     1861 tctggtataa catccagtaa tttttcatat attatttttt gttcatttac tgttctaaga
     1921 atatttatct ctttagttat atctctaaat gtaagcaatt taaagagttt attatctgac
     1981 caatagtctc caactctaac ttcttcaaca tgttcttcac cttctgagtc agacatcaca
     2041 aaattaacta tacagtttgc tgtgtcattg taacttacta tttcagcttt cttttcttgt
     2101 gttagattta atcttttaaa ttccctattg tcaaagaaat ctatgttaaa agtttcaaaa
     2161 tattgttcta atgtcatatc gactaaatca cttgaaacac atttaaaact cttttcatat
     2221 tttttattta tacttattac cttattcttc tttatgccac cttcagcact tcttacaagt
     2281 ataggagatg gaatatttag tataatgttt tcatagtctt tatttatatt tcttatttct
     2341 tcatctattt tgatttcatt tgtaatatca tttaaactta ttaagtaatt gctttcgtct
     2401 ttattattac ttaaacttaa caaataaatt ttatcattta actctaccag tttatcaatt
     2461 atacttatat cgttatcaaa tttcttcaat ttttcagcaa ctttttcatc ttcctttata
     2521 aaagttaata ttttataata attattcata tcctttgaaa attcatcttt aaatttatta
     2581 tttgtgtaac ttatattaaa atcatagtct gttactatcc atacatttgg tattgtatcc
     2641 agagcttgtc taagtaattc ttttctctgg tctatgtagt tttttaatgt tctttgtata
     2701 atcatatcct tttctattgc taaatttatc tttcctaatt gctctgactt tttacttaat
     2761 tttttattga ttgaatcaag ttcataatat ggatacacta ctctattatt aagactgtat
     2821 acatatacat aataagtatg gttacataat agtaaatata ttactcctat taatctatct
     2881 gaatctacta tattaatctt agaaattgct acaaaacata taatagttgt aaaaaaaaca
     2941 aaatatgtta aatcttttat taacttatag tgctcaatgt tactatcaag tttattcaac
     3001 aaatatagta aattaaacat agatatacaa atattaatta atataataat catattccag
     3061 ttaatatcac taaaaatatt tcttataaag taatctaata tcacaaaagc actataaaaa
     3121 attagtattg ggcttattct tagttttttt actttagaaa tggttaagta tataaaagta
     3181 gacatattaa ttatgcttaa gttaagcaat acaatttgga atttttgaat ttttaaattt
     3241 actactccta aaatacttac aaaaactaat acaagacacc ctactttcaa tatatttata
     3301 aattcatctg ttttaaagct acttatataa actatagaat atccaaatat ccaaagtaaa
     3361 attattctat acatatttat agaaactgta tacaaattta tatttaaaag tgcaatagcc
     3421 aaaaataacc cattagctag tatatagacc ttattcttac ttaaagttcc tttagattcc
     3481 atattcccca ccacttcttc atttttatat agttttacct ataaatatgt taccacaatt
     3541 cttccaaaaa atttgtcact ttctagttat aaaaaaaaat gagtaaatat ttttaatttt
     3601 actcattttg tagattttgt atattcagtt taaaaagctg gaactatact tcctttatat
     3661 tcatcttgta taaatttttt aacttcttta ctattcatag cctctgataa agccttaatc
     3721 ttatcactat ctttattatt ttctctacaa gctattatat ttacatatgg agaatcactt
     3781 gattctatta ctattgcatc cttagtagga tttaagttag ctgttaaagc ataattagaa
     3841 tttattactg ctccgtcaac atcttttaat actgttggta attgctctgc attcatttct
     3901 tttattacta tatttttagg atttttagtt atatcttttt tagttataag ctctccatct
     3961 tttacttcta tcaatttatt ttttgctaat aatttaagtg ctctagctcc attagttgca
     4021 tcatttggca cagctataac agctccatct tttaattcat ctaatgactt aactttttct
     4081 gaatagaatc ccattggctc tatatgaact ttagatgtat aagtaagttt atatcctttc
     4141 tccttcacag tttcctctaa aaatggaata tgttgaaaga agttagcatc caaagaccct
     4201 tcatctaaag ctgtatttgg aagtacataa tcatcaaata tttttacttc taaatcatat
     4261 cctttttctt ttaacaaagg ttttgcaact tccaatatct ttgcatgtgg atttgatgaa
     4321 gctcctacta gtattttttt atcctctttg tttgaacaac ctactgctga tattgctatt
     4381 gctgacacca atgctactga taataatttc tttaatttca tttttactcc ccctctttaa
     4441 aatgtttata attaatttat tttagtttct tataagccag atttcctact ccctggatta
     4501 tctgaactaa tattataagt gctattacag tatataccat tactgctgta tcaaatcttt
     4561 gatatccata aataagtgct atattaccta atcctccagc tcctactgca cctgccatag
     4621 ctgtataacc aagtatagat attattgata atgtaattcc agatactatt gaaggcatag
     4681 cttcctttat cataacttta aatactattt gtctttttgt agctccaaaa gattttgctg
     4741 cttctatcaa tcctttatca acttcattta atgagctttc tataattcta gcgataaacg
     4801 gtgctgcccc aatagttatt ggcactattg cagcagtttc ccctatactt gttccaacga
     4861 ttaatctagt tattggaatc attgcaacta taagtatcat aaatgggaaa cttctaaata
     4921 tatttactat aaatcctaat gcactattta ttgtgctatt tggttttaat ccatctggtt
     4981 ttgtaactac taatatgatt gctaatataa atcctaatat tgttgccact atagttggta
     5041 ctataaccat atacaaagtt tgtaacaatg catttggaaa tagtgttgtt aaaaagtcaa
     5101 ttaaactatt catttatcat ttcctcccat cttatgcctt tactagttag atattgtttt
     5161 acttgctctc cacttttatc tgaaatgttt ataataagtg aacctaaaat atcatcttta
     5221 aattgttcta atttcccaaa tattatagac acatctatat ttagttctct tgccatagtc
     5281 gtaataatag cttcattaga aatatcttta gggaatagta tctttatatt tgttcctttt
     5341 ggcaatatta tactttcttc acctattaat tttctaagtc ctttagtatt tcttaagaat
     5401 atttcttctg tatctcctac ctctaatact tctccatttt ccattattgc tactcttcca
     5461 caaatctgct ttataacttc catttgatga gttacaacta ttattgttat tcccaatttt
     5521 ttatttatat cttcaagcaa tgaaagaatt gactttgtag tatttgggtc taatgctgaa
     5581 gttgcttcat cacaaagaag tacttgtgga tttagtgcta aagctctagc aattgcaacc
     5641 ctttgttttt gtccgccact tagatttctg ggtttatcat ttttcttttc agatattcca
     5701 actacttcta ataactctaa aactcttttt ttaatttctg cctttgagta tccaaaacat
     5761 tctaatggca atgctacatt atcaaataca gtttttcttt caagcaatga aaagtgttga
     5821 aaaatcattc ctaactcttt tcttaaatct ctcatttgct tttcattcaa tgactttact
     5881 tctttatcag aaacaagtac actgccttct tggtattctt ctaatccgtt tatacatctc
     5941 agtagtgttg atttccctgc accactatga cctattattc caaaaatttc tccagattct
     6001 atttcaatac ttacatcttt aagcacttga atctttccat aatacttatt aacatttttt
     6061 atgctaatca taaaatttcc tcccatatat tctgctcagt ttttatgttt atcctctctt
     6121 atgtattaca acaaaataaa agcccgagta taaactcagg cttagaaagc tatttatact
     6181 catctatcaa ctatattagt tgcaggattt agcaccaatg tatataaaat atactggttg
     6241 ccgggtttca aagggccagt ccctccacca ctcttgataa gacgttattt aatttaattc
     6301 tattatcatt tgttgctatg ttatatatga tatattcatt aatagtattt gtcaataaat
     6361 ttatttaaat tttttatttt ttattatcgt taattaacta tttttaatta gttttctagc
     6421 tcctaacact ttatatttta atgtctatat aactatagaa catcattata agaaataaat
     6481 taaataacat caatctatct aaatcatctg taaagtaaat taagaaaatt aacattttat
     6541 ccaaactcta tcctactatt aaataaaata atttaaatac aatgatgatt atatagctct
     6601 agtactttag actttatcaa atgtttaagt tctataggtt ctattattgt agcatttttc
     6661 ccaaaagtta aaaaatattc agccacccag ttaagtgtag attttggtat atacatatct
     6721 atgtatccac ctttagacaa gattttcaat cctcttgcca acaaaaattc tgtttcacaa
     6781 cattttactc cctcatctgt caaattaatt ttaatatgat aatctgtacc tacttccata
     6841 ttcttaagat aatcatgtat agaagataaa accttatatt tcttacttga taagttttct
     6901 atactcaaaa tcttcacaat tctgtctaca cgaaattcct taatagattc tgattttata
     6961 cagtaagctg gacaatacca aagtccattc atggcataca aaccaatagg aacaatggta
     7021 cgtacactat ttttttgctt tgatgaatat tcaattttaa ttgtagattc attcattact
     7081 atattaaaca attctttcaa taatggtgtt gaacagtgcc tatctggtgt ccaaaaaaca
     7141 aatttacgcc ttatattctc aatattatat tgaatatcat ttggtataca atttaaaaat
     7201 ttttttaaaa cagaaatagt ttcttgctca aatggcaaat cattataata ttctagtgac
     7261 tgatatgcaa agaatataga ttttacctca ctttctgaaa atgcaatcgg tggaagtata
     7321 cgctctttaa gtacatgata tccaccagcc gctccaactt ttgaatataa tggaaatcca
     7381 atactctcca gcacctgtaa gtctcttaat atagtacgtt ttgatacaga aaattcttct
     7441 gctaattctc ctgctgtaaa acttcttttt gcatttattg ttatcatcag ttcaattaaa
     7501 cgctcagctc ttgacatatt tatactccta atttaaatat agtgacaata attgtcacta
     7561 ttatatgata agatattctt acatttaaag caataataat agtaaagaaa gaagggattg
     7621 atattttatg gaaaatgata ctattaaggc tgatgatatt ctcaattatt gtctatcaaa
     7681 cttagatgat gttgtactaa tggatagttg gggggaacga gcaatttact acaatcctaa
     7741 tggtgtttta aagcgagggg tatatgttct taccattaag gaaaaagaca gtaataatga
     7801 taaaggttcg ttagttagtc gtccaaatgt ataccgtgtg aatataggat taaaaaaaga
     7861 aacttttatt gaaatgtttg gatatattcc aaagcgtcca ggtgtaggtc aaatagttga
     7921 tatggatttt gattttacaa aattggacac aatcatgcca catcctatct actcatggat
     7981 gggatggata tgtgtcctaa gccctactga aaagacattt gagaacttta aaactttaat
     8041 aggagaatct tacaattttg caaaacaaaa atttaaaaag agaaaaaaca agtaaaaacg
     8101 tgtgaaaata acctctaaat aaattataat aaaaatatta aggttacaag ataaaaatta
     8161 atatcttaat aacatgcaaa aaagcgaagc caaaaaatgt attatttata aggcttcgct
     8221 atatctcaaa taatttattt caaatttcaa aactatttat tctattattt cttattattt
     8281 cctgttattc ctaatctatc attcatatct tttatcattt ttctaaaacc actacctata
     8341 tcatttccaa catcttcagt tccatgaact aattttataa agtcattttg accttttttc
     8401 atatcttcat cagatacctt atctaatttt tctattctct tatctatatc ttttactaat
     8461 ctttcccctt cagcgattag gtcatcattc attttcttta actctgtatc ttctgtttta
     8521 ccatcttttt taagagatgt tacaaatgat tgtattttat ctctactatc tttatatgca
     8581 gcctttacat cacttagata tttttcattt cctggatatt ctttatcttt aaactgatta
     8641 tttatacttt ctggagtatc ataaatagaa tatctattta gacctgctat ataatcatta
     8701 tagcttgttg aataataatt catatatcct tcattattat ttacagattc attaggattt
     8761 tttcctgtat tttcacttgg tttttcatta tttttagtac atgcagttat acctatagct
     8821 aatactagtg ctaatcctac aaataaagtt ctttttaatt tca
//
LOCUS       DACXMZ010000163         8846 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.164, whole genome shotgun sequence.
ACCESSION   DACXMZ010000163 DACXMZ010000000
VERSION     DACXMZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8846
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.164"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            115..2016
                     /gene="cooS"
                     /locus_tag="JD016_14350"
     CDS             115..2016
                     /gene="cooS"
                     /locus_tag="JD016_14350"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HAU5000811.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            2009..2482
                     /locus_tag="JD016_14355"
     CDS             2009..2482
                     /locus_tag="JD016_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAU5000812.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            2457..3683
                     /locus_tag="JD016_14360"
     CDS             2457..3683
                     /locus_tag="JD016_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAU5000813.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSI"
     gene            complement(3822..5009)
                     /locus_tag="JD016_14365"
     CDS             complement(3822..5009)
                     /locus_tag="JD016_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAU5000814.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            5282..6106
                     /locus_tag="JD016_14370"
     CDS             5282..6106
                     /locus_tag="JD016_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAU5000815.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVYKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            6235..7500
                     /locus_tag="JD016_14375"
     CDS             6235..7500
                     /locus_tag="JD016_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HAU5000816.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            7713..8303
                     /locus_tag="JD016_14380"
     CDS             7713..8303
                     /locus_tag="JD016_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HAU5000817.1"
                     /translation="MHFKSKDFHLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYDLTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFTYADAPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            8633..>8846
                     /locus_tag="JD016_14385"
     CDS             8633..>8846
                     /locus_tag="JD016_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HAU5000818.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYCTFTINILRSKKHIN"
BASE COUNT         3413 a          968 c         1595 g         2870 t
ORIGIN      
        1 ggtatggtaa atcgtggtat taaataagta gattttaata gttaaattat agtcaattat
       61 aataaagcta tttaatctac ttttttaata taaaaatata agggggaatt tgtaatggct
      121 tgtaacactt gtaaaatgtg cgaaagtgca gacaagaaat tagaaagttt tgtagcttca
      181 aaggatgttg aaacagcatt tcatagaaca gaagatcaga aagttaagtg tggttttgga
      241 cttcaggggg tttgttgtag actctgttca aatggaccat gtagagtaac tcctaaatca
      301 ccaagaggta tatgtggagc agatgcagac actatagtag caagaaactt tttaagagca
      361 gtagcatcag gagcagcttg ttatttacat gtggtagaaa atacagctaa gaatttaaaa
      421 aatgttggaa taactaaagg tgttgttaaa ggagaaaaaa cattaaatca attagcagag
      481 atgtttggaa tagaaagtga tgaaaagtat gataaatgta taaaggttgc agacaaagtt
      541 atcaacgatt tgtataaatc aagagatgat aaaatggaat tagtagaaaa aatagcttat
      601 gcacctagag tgaaaaagtg gaaagagtta ggtataatgc ctggtggtgc aaagtcagaa
      661 gtttttgatg caatagtaaa atcatcaaca aatttaaata gtgacccagt agacatgcta
      721 gttaattgtt tgaacttagg tatatcaaca ggattatatg gtcttacact aactaatctt
      781 ttaaatgatg ttatgttggg agaaccagtt ataagaatgg ctcctgtagg atttaatgta
      841 atagacccag actacataaa tataatgata acaggccatc aacattcaac atttgcaaat
      901 ttccaagata gattaaaaga tgaagatgtt ataaaattag ctcaatctgt tggtgcaaaa
      961 ggctttaagc tagtaggatg tacatgtgtt ggtcaagatt tacaattaag aggagaacat
     1021 tatcaagaag tatttgctgg acatgcaggt aacaatttta caagtgaggc tgttttatca
     1081 actggagcaa tagatatagt tctttcagaa tttaactgta ctataccagg gcttgaacca
     1141 atagcagata aatataaagt aaaaatggtt tgtttagatg atgttgctaa aaaagcgaat
     1201 gcagaataca taggtttaga cagaagtaaa ttagatgaac ttagtaacac tttaatagaa
     1261 aaagcattgg agtcttataa ggagagaaga ggaagtattg aaatagatat accaaaagac
     1321 catggatttg aacaatcttt aactggtgta agtgaaaaga acctgaaagc gtttttagga
     1381 gattcatgga aacctcttat taatttaatt gcagaaggaa aaattaaagg tgttgcagct
     1441 gttgtaggat gttcaaatat gacagcagga ggacatgatg taaatactgt tgaacttact
     1501 aaagaattga ttaaaaaaga tataatagta ctatctgcag gatgttctac tggaggatta
     1561 gaaaatgttg gtcttatgtc acctggagca gaagaattag caggagaaaa cttaaaagaa
     1621 gtatgtaaga ctttaggaat accaccagta ttaaactttg gaccatgtct agctatagga
     1681 agactagaaa tagtagcaac agaattagca gctgaacttg gaattgacct accacaatta
     1741 ccattagtgc tttcagctcc acaatggttg gaagaacaag cattagctga tggagcgttt
     1801 ggtcttgccc ttggcttacc tcttcattta gctttacctc catttataac aggaggaaaa
     1861 ttggtgacag aagtattaac agaaaaatta aaagacttaa caggaggaca tgttatagtg
     1921 aatccagacc ctaaatcttc agctaatcaa ttagaagaaa taataataga tagaagaaaa
     1981 aatttagggt taaaggatgt ggaatgcaat gcatagaatt tttataaata aagatttatg
     2041 tacaggatgt aagagctgtg tactagcatg tatgctaaaa cacaataaag attatgacat
     2101 gtacactctt gatttagaaa atatagataa tgatagtaga gggcatatag aattagatag
     2161 taaacataat aatccagttc caattctttg tagacactgt gatgaacctg aatgtgtatt
     2221 agcatgtatg agtggtgcaa tgcataaaga tagtgaaagt ggaatcgttt catatgatga
     2281 agagaaatgt ggttcatgtt atatgtgtgt aatgtcttgt ccatatggat tattaaagcc
     2341 agatgataga agcaagcaga acatacttaa atgtgattta tgtaaagatg aggagtatcc
     2401 tagatgtgta gctaattgcc caagtggagc aattgaatta cagaaggagg aaaatgatga
     2461 attatgtagt gttaggggct agtgcagctg gtataaatgc agtaaaaaca ttaagagaat
     2521 tagataaaga ttcaaatata gtagtaattt ctaaagatga aaacgtatat tcaagatgta
     2581 tgttgcatca tgttatatct gaacacagaa ctttaaaaca aataaatttt gttgatgaag
     2641 attttatgga gcaaaataat gtaaaatgga ttgcaggtaa gactgttaaa ggtatagaca
     2701 taaataaaaa ggtagttcag acagaagaca ttactgtaaa ttatgataaa cttttaatag
     2761 ctacaggtgc atcttctgct attcctccta taaaaaattt aagagatgga aattttgtat
     2821 attctgtgag aaacatagat gatatttata aaataaaaga aaaagcacaa aattctaaaa
     2881 atgtagttat aataggtgct ggtttagttg gtatagatgc attagtggga ttatttaaat
     2941 atgagcagtt aaatatttct gtagctttta tggaaaagta cattttagac agacaattgg
     3001 atgaatatac agcatcagtg tatgaaaata agttcaaaga aaaaggtgta aaattttacc
     3061 caagtgcatc aatacaagaa attgtattgg ataacagtaa gaatgttaca ggtgttgcat
     3121 tttcaaatgg tgaagtttta gatgcagaca tggtaattgt agctacaggg gttaaaccaa
     3181 atgctgactt tttagatgga acaggaatag aatatgatag aggtataata atagatgata
     3241 tgtgccaaac aacacaaaaa gacatatatg cagcaggaga tgttgtaggt aaaaatgcta
     3301 tttggcctct ggcagtaaag caaggaattg tagctgccta taatatggtt ggaaaagata
     3361 agaagataga agatgaattt gcttttaaaa acagtatgaa tttcatggat ataccaacta
     3421 tttctatagg aatgaataca cctgtagatg atagctataa agtattaacc agacatggaa
     3481 ttaatgatta taaaaagttt gtttttaaag ataatgtaat ctatggagca gttatacaag
     3541 gagatatttc atatgtaggt gtattaactt atttaataaa aaataaagtt gaaatatatg
     3601 atttagaaaa taggatattt gatgtatgtt atgcagactt ttttaatata aaagaaaatg
     3661 gagaattttg ttatagtata tagtatatac gtgaagtaag gtttataaat acagtaaatc
     3721 aataaataga gttattctaa aataagagaa atgacaaatc ttatttagaa atagctctat
     3781 tttttgcaat taaatttaaa ttatattcaa tttgtaaaat attattttga acaattatac
     3841 atatactctc tagttagagg taaatcattt tttatacctt tagtgaaaag tatttgatgt
     3901 atatctataa taccagtgtt gaaagcagca gcacaagaac aaaggtataa atcccacatt
     3961 cttataaact tatcatcaaa catttcttta atctttggaa gattctcaga gaaatttttt
     4021 tgccagtata gtaaggtttt tacataatgt aatcttagac tttctacgtc tatagtatga
     4081 tatttgtaat cactacaaat atgtatcatt tctcttaggc taggaataac tcctcctggg
     4141 aaaatatatt tttttatcca agggtctcca taagattcct gcaatccact tatatagtga
     4201 agtaaaaaca atccaccatc ttttaaaaca gcatttacgt ttttaatgaa aagttcatag
     4261 ttttctctac caacatgttc aagcatccca acgcttacaa ctctatcaaa cataaggtct
     4321 gattttttta attctctata gtccataagt cttacctcaa gatagtcttg aaggttctct
     4381 tctttaattc ttgcgttaaa ttccttaaat tgctcttcgc taagtgtaat tccaacgccg
     4441 tgaattttat atttttttgc agcttctatt agtaaaaatc cccaaccaca accaatatcg
     4501 agaaggctca taccttcctc taaatttagt ttagttaata tgtggtgaac tttattgact
     4561 tgagcatcat aaagagtatc attatcattt ctaaagtatg cacaagaata actaagtgtc
     4621 ttatctaacc ataagctata aaaatcgttg ccaatgtcat agtgggcatg tatttctttc
     4681 ttttgatgtc tcattgaatt tgatgagtgc aatagatgtt ttacagctga attatcaata
     4741 ttgaatttgt ctatttgttt taaaagtaga tttaaagtta aaaataaatc tccctcaact
     4801 tcaatgtctc tatttatgta ggcttctcca agtgctaaag atgtactttt taatagttca
     4861 gacttgctaa tatcattatt tactataact ttgaactgag gggagccctc tccaattaga
     4921 aattcttcct catctttaag cctaactaga aatggtgttt caccaaattt ctctaaatag
     4981 tcttttaaaa aattttttgt aatattcata ttaccagctc cttctaatgt aaatccagta
     5041 agtttccctg cttaaaatat attatagtcc tttttttaaa aaagtaaaat tttttcaata
     5101 aattttttat ttaaaggaaa tttatttttt gtaaagaaat ttcatgttgt agtatatttt
     5161 ttgcttataa attttggtac tggatataat attaaactgt taatgatata ataaatttaa
     5221 tctataaagt aaataaaatt ttgtaataaa tagttaaagc tattttataa ggagatatat
     5281 gatggaagta tatgctttgg ttgggtcaag tggcacaggg aagagcttta aggctttaga
     5341 gttcgcatat gaaaatgata tagaatatat aattgatgat gggattttaa tatacaaaaa
     5401 caaggtttta gctgggatat cagcaaaaca agcaaatact actatagaag cagttaaaag
     5461 ggctattttt tataatttgg agcatagaca agaagttaga gaagcaatta aaaaagaaaa
     5521 tataagaaaa attcttatta ttgggacatc aaaaaaaatg gttaatcaaa ttgtagagag
     5581 attgtcaatt ggaagggttt ataagtttat aaatatacaa gatataagta ctaaatatga
     5641 aatagaaata gcaaaacaag ctagaaaaga aggaaatcat gtaattcctg ttccagcagt
     5701 tgagataaaa agtatggcaa gtggtcttag tataaattct ttgaagagac tttttagaaa
     5761 aggaaataat aggaatatgg tagttttaga aaaaacaatt ataagaccaa cttttagtta
     5821 tataggaaag ttttatatta gtgctgatgt aattaaacag attattgaat atgaagtata
     5881 caactttgga agcattgata gaataaataa aatcaatata gagaattgca ataattttat
     5941 gaatattttt gtaagtataa atatcaatga tttagaaact ataaaaaaaa ttgaagacat
     6001 acaacagaca ataaaaaagt ctattgagaa aatgacttta gttaatgtac aaaaaataga
     6061 tattaacatt cataaaatta aaaaaataaa cttcctccaa tgttaaaaaa taaactgaga
     6121 aaatgatata ctaatttgca ataaagtgtg ctataattaa actgtaaatt taggaaatgg
     6181 ttttttctaa atggagtata cataattata acgtatttat aagggggctt tcaaatgtca
     6241 ggaaaagatg taaatgtctt cgagatggcg caatctcaag taaaaaatgc atgtgataaa
     6301 ttaggtatgg aaccagcagt ttatgaatta ttaaaagaac ctatgagagt tatagaggtt
     6361 tcaattccag taaaaatgga tgatggttct ataaaaactt ttaaaggatt tagatcacaa
     6421 cataatgatg cagtagggcc aacaaaaggt ggaataagat tccatcaaaa tgtttcaaga
     6481 gacgaagtaa aagctttatc tatatggatg actttcaagt gttctgtaac aggtatacca
     6541 tatggtggag gtaaaggtgg aataatagta gatccatcaa ctttatctca aggtgaatta
     6601 gaaagattaa gtagaggata catagatgga atatataaat taataggtga gaaagttgat
     6661 gttcctgcac cagacgtaaa tactaatggt caaataatgt cttggatggt tgatgagtac
     6721 aataaattaa ctggacaaag ttctataggt gttataactg gtaagccagt tgaatttggt
     6781 ggttctttag gaagaacagc tgctactgga tttggtgttg ctgttactgc aagagaagct
     6841 gctgctaaat taggaataga tatgaaaaaa gctaaaatag ctgttcaagg tataggaaac
     6901 gttggttctt atacagttct taactgtgaa aaacttggtg gtactgttgt agctatggct
     6961 gaatggtgta aatcagaagg ttcttatgct atatacaatg aaaatggttt agatggtcaa
     7021 gctatgttag attatatgaa agaacatggt aacttattaa acttcccagg agctaagaga
     7081 atatctttag aagagttctg ggcttcagat gttgatatag taataccagc tgcattagaa
     7141 aactctataa ctaaagaagt tgctgaatct ataaaagcta aattagtttg tgaggctgct
     7201 aatggaccaa ctactccaga ggctgatgaa gtatttgctg aaagaggaat agttcttact
     7261 ccagatatat taactaatgc tggtggagtt acagtttctt acttcgagtg ggtacaaaac
     7321 ttatatggat actactggtc agaagaagaa gtagagcaaa aagaagaaat agctatggtt
     7381 aaagcatttg aatctatttg gaaaatcaaa gaagagtaca atgttacaat gagagaagct
     7441 gcttacatgc attcaattaa gaaagttgct gaagctatga aattaagagg atggtactaa
     7501 gatttactct taataaataa taggatataa ttatttaaga tataaagggg ctgtacatag
     7561 tacagccctt aaattatgtg gcttacaatg aagtaagcta tttttgtttt aacttataaa
     7621 gaacttgata aattatgtaa tttagaatgt attttttacc accacatgat tacaaattag
     7681 aagagttaga ttgaattttt agacatgaac ttatgcattt taaaagcaaa gactttcatt
     7741 taaagtatct agtattattt ttaaaaatag tatattggtt taatccattt atatatataa
     7801 tggaaaaata tatggatttg gattgtgaac tttattgtga tgagagagtt ttaaaaaatt
     7861 gtactgtaca acaaaagcaa gaatatgatc taactattgt aaattcaatg aaaagaagta
     7921 caaatttttc aaataaattt gtagctggat taaataaaca atctgatatt agaaaaagag
     7981 taataaacat gttcagtgaa aaacataaaa atggtatctt aagggctttg attataagct
     8041 tgttttcttc tattacattt tttaaattcg attcaactag tattattgaa gagaatgatt
     8101 tcttagagag gtcaagatat acgtttgaag gaaggcaaga agacatgttt gtaacaatag
     8161 attttacata tgcagatgca cctataaaac ttagaaaaga gcatgaagaa aactgtagag
     8221 tcaatggaat agtaccaaaa gattcagatt caatagaaat tattcctaga ttttataaag
     8281 attgtatgga tttaaaaaaa taagttagac ttaatattga tgttgatatt atgaattttt
     8341 tatatttaag caattgaaaa ttttattata gattcaattt tatatgtgtt taagtatgaa
     8401 atttatagat agttgaaata attaaatatg attgatgcat aggaatcatt actaagctat
     8461 aattaattat aggggagttt aaataaatta tgagttacac aattataaaa tgataaaaat
     8521 agttatctgt tacaaaaaat gtatcttttt aattaataaa gataatatga aaacttaaaa
     8581 ggcaaataaa cttaaatata aaatactctt aaataagata ggagaaaaat gtatgtctaa
     8641 ctttattcta aatgggattt ttagaacagt tatagtaagt agtctaagca ttcttctgat
     8701 attgattttc aagaaaaatg tatttaaaag atttagcaaa aaatttaatt attatatatg
     8761 gatgatagtt gttattaaat tatttttacc atttacatat tgtactttta caataaatat
     8821 cttgagaagt aaaaaacaca ttaata
//
LOCUS       DACXMZ010000164         8841 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.165, whole genome shotgun sequence.
ACCESSION   DACXMZ010000164 DACXMZ010000000
VERSION     DACXMZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.165"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1735)
                     /locus_tag="JD016_14390"
     CDS             complement(<1..1735)
                     /locus_tag="JD016_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HAU5000819.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSD"
     gene            complement(1749..2654)
                     /locus_tag="JD016_14395"
     CDS             complement(1749..2654)
                     /locus_tag="JD016_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAU5000820.1"
                     /translation="MQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYFYGKDILSM
                     CIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTLIGTDTFID
                     NEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPGYERVNLHP
                     ATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDADEEKLIHF
                     AGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGKTFDVYEKE
                     MVGDVIKTRIPNKWESIDVFPISHI"
     gene            2906..3094
                     /locus_tag="JD016_14400"
     CDS             2906..3094
                     /locus_tag="JD016_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU5000821.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            3087..3788
                     /locus_tag="JD016_14405"
     CDS             3087..3788
                     /locus_tag="JD016_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000822.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            3790..4713
                     /locus_tag="JD016_14410"
     CDS             3790..4713
                     /locus_tag="JD016_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU5000823.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            4758..4877
                     /locus_tag="JD016_14415"
     CDS             4758..4877
                     /locus_tag="JD016_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000824.1"
                     /translation="MARFWQGSCFIILIVVDFDVVSTIFCRNKFIKSEEIIYE"
     gene            4870..5793
                     /locus_tag="JD016_14420"
     CDS             4870..5793
                     /locus_tag="JD016_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5000825.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVIVISSQLCNDTLEDYFKKITGGEGIA"
     gene            5786..6523
                     /locus_tag="JD016_14425"
     CDS             5786..6523
                     /locus_tag="JD016_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000826.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            6534..7307
                     /locus_tag="JD016_14430"
     CDS             6534..7307
                     /locus_tag="JD016_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5000827.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(7614..7826)
                     /locus_tag="JD016_14435"
     CDS             complement(7614..7826)
                     /locus_tag="JD016_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000828.1"
                     /translation="MYKVIKTVEKIEDVVNLHKHYVFVGSRRFFYCLCLWYFFLKMYH
                     VLHFVEDKFAYIEEKAAALKIKTTTL"
     gene            complement(7848..8456)
                     /locus_tag="JD016_14440"
     CDS             complement(7848..8456)
                     /locus_tag="JD016_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAU5000829.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(8634..8750)
                     /gene="rrf"
                     /locus_tag="JD016_14445"
     rRNA            complement(8634..8750)
                     /gene="rrf"
                     /locus_tag="JD016_14445"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2914 a         1472 c         1283 g         3172 t
ORIGIN      
        1 catcagaaaa tctagttata tctcttgaat atataaaatc atttccatat attaatcttt
       61 cagcttcaag ttctgattct tttcctgtaa atactcctaa tactaatata ttttgctttc
      121 tagcttttct cacttcattg gctgtatctc tcaaaccaac tataccccta taagcctttt
      181 ctcctcttaa tgttctctca ctactttttc ctatctttac atcatttggt cttccatcgc
      241 tgagaactat caggatttta ttttcttcat ctctatttat tagaccacta catatagatt
      301 ttattgccaa accatctcta ttattaccag cacaaaaata ttcaaatata ttttcactat
      361 tttttatact atcatcataa tctttaaatc tttttaatat tgtatagtct aaaaaactag
      421 aaaatcccat cactctattt ggtatgccaa cttctgttag agcatttgca attatgtaag
      481 cttgaattgc cacatttccc tgatttctac tttgagagcc acttgcatcc aataatatat
      541 caataacgta tttccctttt tcattttcta tatctttata aaatatttta ttattattac
      601 ttctaccaac tctccaagct ctattggcta atatagtacc acaatcagaa tatactctac
      661 ttttagcatt ttcttctatt aaaattcttg acagactttc ttttaatctc tttatttgct
      721 gtctatacat tctagcatgt tctctaaatt ttcctaaatt attttctttt tgtcttgtta
      781 catattttag ttgaaataca ttattacact cacttctaag aactccatct gtaaagtgta
      841 ttctacaacc ttcatgaaca tttctacaat tttgtgtctc aattttttta atttcacctt
      901 tactcaaata agtttttcca taataatgtt ctatttttga atatatctta tttgcagtgt
      961 cttcatcaac atatataact cggtctgctg aattgctaca tttagaatca tcaaaatccc
     1021 ctacaccttc tacaagcatg gatgaagata ttgaatctat atcactttca ataatatttt
     1081 cttcttcatc actataaagt tcttcataca tgaaatcagc aaatgtatca aaatttactt
     1141 ctacactttc tacattattt tcatcaataa gactattttc ttctccattt aatatgtatt
     1201 caaaataagt taaatatata acatctaatg cttttaaaat ctccataata tcttgattgg
     1261 tatctatatt tcttatatca ttaactattc ttctagtcat actatctaat gctgggtgct
     1321 tgtccatact ataaagtaca aatgtgtatt taattttatc cataatatta tcattgtgta
     1381 ttttggtaaa acgtgaaaca atatctttat aagcatttct tcttatgtct tctatccctg
     1441 gcctgtcttt aatgaccttt tcttcaacta ttgtatttaa tataacttga attagaccta
     1501 gtataacatc tttgttgtat ccttttctta ttctatttgt aagatatttt ttaactatat
     1561 cccaatctac atactttctt ctagcacctg caattattgc gaaatataga cctatttctt
     1621 ttgaagagta atctttttct gatatatcta tactttcatt gtaatcacca gctattgtcc
     1681 atgataggtt attagctcta ttttcaaatt cataatcatc atatatccat ttcataatct
     1741 cacctatatt aaatatgact tattggaaac acatctatac tctcccattt attaggtatt
     1801 ctagttttaa ttacatctcc aaccatttct ttctcatata catcaaaagt ctttcctgta
     1861 agtcccatat taattgcaag agttggtttt aatcctcttc ttatagtctt aagagatgcc
     1921 ataagaccac gtaaatcaat agctttacta gatatctctc cattttgaga cttcaattgt
     1981 aaatctaaaa atattcctgc aaagtgtatt aatttttctt catcagcatc tgaaaattca
     2041 ttttttaaaa tcatcattaa cttatcttct tctataggag gaatatctat taccataaat
     2101 ctagatacca gtgcttcatt aagttctttt gttcctgcat attcataatt catagtccca
     2161 ataaatcttg ttgctggatg tagattgact ctctcatacc caggtacatc aattattctt
     2221 ctatagtcta aagctgaatg taccacaact atagcatcat tttttgccat attaatttca
     2281 tcaaataccc caaaacctcc attaatagca cactcataaa ctgagcctct tctaagttta
     2341 acttcattgt ctataaatgt atctgtacct ataagtgttg aactgtctgt atttatatga
     2401 aatgatgtat tccactgagg tctattaaat atttccccta gattatcagc aagtaagttt
     2461 tttcctgttg cctttggtcc cgataataat atattttctt cttctaaaat agctgctata
     2521 cacatagaca agatatcttt accataaaaa taagttttag actttgttac tctgtattct
     2581 aaatcttttt ctaatccata ataatttcta aaatacagta catcttcaat aagtttttca
     2641 tctacccctt gcacccttag attatctatt atatccatat ttttacccct tttatataaa
     2701 tagtatttgt ttcatttctt atcaccattt tagcataaaa ggatataagc taaaattgat
     2761 tttttaatct ccccatttta agtaaaacca tgcaccattt tatcactaca aaattaagag
     2821 tactacttac tgatatttct tgattttgtt cgtctatacg tatataatat atgctgataa
     2881 attatgagga aaggatgatt gaaatatgga atatatgtct tgcccagaag tagcaaaaaa
     2941 atggggtatt tctgaaagaa gagtacaaaa actatgtgaa gaaagtcgca taccaggagt
     3001 ttcaaaactc gggtatatgt ggctgatacc aaagaacgca gaaaagcctg ttgatggtag
     3061 aacaaaaaga agaaaggaag tacaatatga atagcgtttt gattatagat gatgacaaag
     3121 aactttgtgc tcttatgaaa aaatgtgttg agcaagaaaa tttatctgct attactgcac
     3181 attgtggtgc agaaggtcta catttggtcg ataaaaatag acacacactc tctcttgtaa
     3241 ttttggacat aatgatgcca gatatagacg gttttcaagt tctgaaaaaa attagagaaa
     3301 caagtaatat tccagtaatt atgcttactg ctaaaaatga cgaggatgat aaagtttccg
     3361 gactacgttt aggtgctgac gattacctaa caaaaccgtt cagcataaac gaacttatgg
     3421 ctcgtgtcaa ttctcttatt cgtcggtata ctatgttaaa ccctgctttt ataacagaca
     3481 cagattgcat aataataaat ggcatgatta ttgacaagac aaaccgaatt gtttctgtca
     3541 ataatattcc tgttcaactt acaagcaaag aatttgattt acttgctttt ctggcttcta
     3601 ataagggacg ggtgtttacc aaaaaacaga tttatacaca agtatgggaa gaagaatatg
     3661 cttttgacga tagtaacatc atgtctttca tcagtaaatt gagaaaaaag attgaaccag
     3721 accccaacca tccattttat attttgacag ttcgtggtgt cggttatcgt tttaataagg
     3781 aggcttaaca tgaatacaag tgtctatctg cttttgatat tggttcttag cctgattatt
     3841 atagcgtacc ttgtttctgt cctgctacgt gtccgaatac aacttacact tataaaagat
     3901 gctttagagg atataaaaaa tggaaacctt aatcgacgta ttttaacaga tgaaaacgac
     3961 attacaaaac aaatttgcta tgatattaac gaaatagcaa tcaatagcca atttcaactt
     4021 attcagcaaa aacaatcaga acaagcctac aaacagctaa tgacaagtct ctcccatgat
     4081 gtaaaaactc cacttgcttc tttggtcggt tatttggaag cagtagaaag taaaattgtt
     4141 gtaggagagg aaaaagacga gtatatccat gttgcgtcta ataaggctca ttacttaaag
     4201 aattttgtag aaaatctatt tgaatgggta aaattggatt ctaaggaaca agtttttcat
     4261 tttgatattt ttgacttgaa tgaattatcc cgtaatatta tatctgattg gatacctgtt
     4321 ttagaaagca gtcattttga atatgaattt gatataccag aaatagagta cttcttacgt
     4381 atagacgcaa atgcatatac ccgtatcatt aataacctat tacaaaatat tattacccat
     4441 agtagtggca acaaaatgac attgagaatt tttgaaaaca aagagcaagc tcaaattata
     4501 ataacagata atgggaaagg tatatcttca gacaatctcc cacatatttt tgaaaggttg
     4561 taccaatgcg accattcacg agcttcaaag ggaaatggtt taggcttggc gattgcaaag
     4621 gaacttatta acgctcataa gggaaatatt accgccaaca gcactcccgg tatgggaaca
     4681 gaatttacaa tattgcttcc taaagccctg taaaagtacg aggctttctt actctaaaac
     4741 aagataaaag caagattatg gcaaggtttt ggcaaggttc atgttttata attctaattg
     4801 tagttgattt tgacgttgta tcaactatct tttgtagaaa caaatttatt aaaagtgagg
     4861 aaattatata tgaatgattt tgtgattgaa acaaagcaac ttacaaaaat ttatgggaat
     4921 caaacagtag ttgacaaagt caatctccat gtaaaaaaag gccgaatata tggtctgtta
     4981 ggacgcaacg gagctgggaa aaccacaatt atgaaaatga ttttaggatt gacttcaatc
     5041 acgtatggcg aggtagatgt atttggagag aatattaagg gacgtgaaaa acaagtatat
     5101 ccccgtatcg gtgcaattat cgaaacgcca ggattttatc ctaatctgac aggaacggag
     5161 aacttggaga tttttgcaaa actgcgtggt acagccgccc ctaattctgt aaaaagagca
     5221 ttggaaattg taggcttacc ttataaaaat aaaaagctgt ttagtaaata ttcacttggc
     5281 atgaaacagc gacttggtat tgccaacgct atcctgcatg acccagaact gttagtttta
     5341 gatgagccaa ccaatggtct tgaccctatt ggtattgcgg agatgagaaa gtttattagg
     5401 gacttgagcg ttgagcatgg aaagacaatt cttatttcca gtcatattct ttcagaaatc
     5461 tcattgttgg ctgatgatat tggaattatc gaccacggtg ttcttttgga agaaagtagt
     5521 atgagtgaac tagaaaagaa aaacagtaaa tatatcctgc tacaagtttc agatattccg
     5581 aagacatcac ttattttaga gcgtcaattt aacacgaagg attattccgt tcaagatgac
     5641 tacacgttac acctttataa cactaatctg gatatgggtg aaatcaataa agcacttgta
     5701 atgcagaatg taattgttat tagttctcaa ctttgtaatg atactttaga ggactacttt
     5761 aaaaaaatca caggaggaga gggcattgct taaacttatt caaacagaat ttcttaaact
     5821 gcgtcgtagg aaacttattt ggctgatgtt gttgtctgct ctcattatgc cgtttttcgc
     5881 actactatac ttcaattatt ttggtaaaac aggtgttgag ccaacaaagt tttataaatg
     5941 gtctgcattt ggttatacac tttggattat tttgcccgtt gttttgggta tattttgcac
     6001 catgcttata cacgaagaaa accagtatga tatgctcaaa cagctttgga ttgtacctat
     6061 tagtaaaatg ggatactttt ttagtaaatt tttcgttgtt ttgatttact ctatttgttt
     6121 tatgctgatt acagccgttg cttctgtgtt atttagtgta ctttctggat atgtcgtatt
     6181 tagctgggca agcattttat atttgttgaa aaaatgttta gaaattggtg taatcactgc
     6241 ttttgtaatg ttgccgattt tggcaatcgc tacatctcaa aaagggtaca ttctcccaat
     6301 ttgtatcaca ttggtatatg ccttttccgg ttttgttata atgacaataa atatgtattt
     6361 acacccatta tccagtatgg gagttattat catgagaaat aaagacattc caggtcttat
     6421 attttcacag gaaattaatg tcccattagc atttctttgt atttgtattt ggagtattgt
     6481 atcagtattg tttgcaaaca ttgcactagg aagaagaaag tgaggtgtag ataatgtgga
     6541 aattactttg ggctgaatgt caaaaaattc gtcgttcaaa aattgtttgg attacagttt
     6601 ttgcatccat tatgatagca gtcatagttt ttgcaggagg tcaggatgta tataatgacc
     6661 cagatttaca ttatggctta aagagcgttt atgacggttc acggtatata gacaatgcag
     6721 gttggtatat ggacgcagta caaccttggt caactttttt tgttcttcct gctgtcattg
     6781 ccctattagg aagctatatt atttgtcgtg aagaagagga ggacactatt aaatccctgc
     6841 gtcttattcc cataaatgaa acaaatttaa ccatagcaaa aatgattatt acttttatat
     6901 tcagtatact tctttatttg ttactcttta ctataacatt tttgactgaa tccgttttac
     6961 atttttccga tttatcgaca aaattggttt taagttgcat gaaagagtat tttcttgatg
     7021 gcataggtgt attctttgca atttctccca ttattgcctt agtatcgcgc atgaaaaaag
     7081 gatattggct tgcattagta ttcacagaag tttattctat tgctggacta tttgcgggta
     7141 tgtcaaatgt cttacaaacc ttttttccta ttgcggcaat atttaatatt tctggatact
     7201 atataactac tggagaaaaa attatgggga gtatcataag tttattactt tgtgcctgcc
     7261 tttctgcatt tatactgaaa gggctaaaac acaatgaaaa aaattgaaat atgtatatca
     7321 aaatgaaaaa gataaaaaaa ctgattattc taattgtatt cttgtttctt tttggcatgt
     7381 tacactgtta tactgaatgg aaattcaaca atgacccacc taaaggggta atagaaagaa
     7441 taaatgagtg gactgataag attggactgg attagtaact tatgttaatt atagtgaatg
     7501 taatattagc aagttattgt ttcgtatttt aatactataa ctttaaatat ttggttttaa
     7561 tcttccaaac gaatataaaa aaactgtcac agaggcaaca tatttttgaa tatttataaa
     7621 gtggtagttt tgattttcaa agctgccgcc ttttcttcta tataagcaaa cttatcctca
     7681 acaaagtgta aaacatggta cattttaaga aaaaagtacc acagacacaa gcaataaaag
     7741 aaacgccgag aacctacaaa aacgtagtgc ttatgcagat ttacgacgtc ttctattttc
     7801 tcaaccgtct taatgacctt gtacaaaaga aatttttcta atatttttta taatcttatc
     7861 ttgtgatgtt ttttatgtat aaatgactta ataacaaatc caataaaaat tatggatatt
     7921 ggtataatta gaacgaggaa tttataatca ttataatgtg acattattct tacccaactc
     7981 tcagaaaaaa tatatcctat actgattaat gaagtattcc atatggttat tcccacgata
     8041 gatgatatta caaaagttat aatattttga cctgctatac ctgctataaa agatatgtat
     8101 gttctacata aagggattaa tctacataca caaacagaaa tagaagagta tttattaaaa
     8161 taatcttgag atgcaaatat tcctttcttt gcctttggac atatctctat tattttattt
     8221 atgatagatt tacctcccca atttcccact gtataacaaa atatcgaacc aagtatacct
     8281 gcaattatac ttaaaattaa aattgtcaga aatccaaaat gatttcttgc agcaattgct
     8341 ccacataaag gtaaaacaac ttcgctaggt aatggaaaac aagcatactc aatcataatt
     8401 aatacaaata cagagattaa tccatattgt aaaataaaac tattgattac agacacttac
     8461 ttatctatct cctttcttat ttaaaggttt taagggtaca tgttctaagg attctcaccc
     8521 tatatttaaa gaatcaatct ctttaaacta ttatatgtat ttacacaaaa catttataac
     8581 atactttaaa aatagtacct taaaataaga aacttaataa ataaaaaaag attacgtggc
     8641 tacgtcctac tctcccaggc agtttcccac caagtaccat cagcgctaga gagcttaact
     8701 tctgtgttcg gaatgggaac aggtgtatcc tctctgctat tgtaaccaca taatctttat
     8761 gcttaatatt tctattaagt ttttaataag tttatagaac ctagtaatac tagtttcttt
     8821 agagttagca ctctaaaaac t
//
LOCUS       DACXMZ010000165         8817 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.166, whole genome shotgun sequence.
ACCESSION   DACXMZ010000165 DACXMZ010000000
VERSION     DACXMZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.166"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(83..910)
                     /locus_tag="JD016_14450"
     CDS             complement(83..910)
                     /locus_tag="JD016_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAU5000830.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            1292..1612
                     /gene="sugE"
                     /locus_tag="JD016_14455"
     CDS             1292..1612
                     /gene="sugE"
                     /locus_tag="JD016_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HAU5000831.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            complement(1745..2425)
                     /locus_tag="JD016_14460"
     CDS             complement(1745..2425)
                     /locus_tag="JD016_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAU5000832.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            2821..3873
                     /locus_tag="JD016_14465"
     CDS             2821..3873
                     /locus_tag="JD016_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAU5000833.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKVIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            3929..4336
                     /locus_tag="JD016_14470"
     CDS             3929..4336
                     /locus_tag="JD016_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAU5000834.1"
                     /translation="MAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSIDEF
                     SIKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPMLLEL
                     FLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            4365..4838
                     /locus_tag="JD016_14475"
     CDS             4365..4838
                     /locus_tag="JD016_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU5000835.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            4885..5643
                     /locus_tag="JD016_14480"
     CDS             4885..5643
                     /locus_tag="JD016_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAU5000836.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            5667..6506
                     /locus_tag="JD016_14485"
     CDS             5667..6506
                     /locus_tag="JD016_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAU5000837.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMCILIVVGAITGIL
                     GLF"
     gene            7043..8182
                     /locus_tag="JD016_14490"
     CDS             7043..8182
                     /locus_tag="JD016_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HAU5000838.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            8208..8396
                     /locus_tag="JD016_14495"
     CDS             8208..8396
                     /locus_tag="JD016_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000839.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT         3292 a          980 c         1526 g         3019 t
ORIGIN      
        1 aaattaattt taaatcaaat taatgagaac cccataaatt tttttaacac ttaatttacg
       61 atttatgggg ttatgttctg ttttatttca aagtattaag ttcatataaa tcagttcttc
      121 tgtgcttaag aagtggtaac tgttgtctta tgtcaggaat taaatctcta tcaatgtcag
      181 cataaataat acattctttt tcatcagctt gagcaattat tcttccccaa gggtctgaaa
      241 ttcttgaatt tccaaaagct atataagaag cattcatatt tctagcaggt gcagcaccaa
      301 catagaatat ctgattatct aaagcacgca ttcttataga taactcccaa tgggcgggac
      361 cagtagtcat attaaatgca gcaggtaaga taactatttc agcaccttta agagccatta
      421 aacgtgataa ctcagggaaa cgaatatcat agcaaatagc tataccaact tttccatatt
      481 cggtatctat tacagtgaca tcatttccag ctgtaagagt atctgattct ttaaaactaa
      541 cttttcctgt aacttcaatg tcaaataagt gcattttacg atgttttcca attagagctc
      601 catttttatc aaatacatag caagtattat agatttttcc atcttctaac tcaggaatac
      661 taccagaaac tagataaatt ccatgtttct cagctaattt acttaatatc gttgttgttt
      721 cacctggata ttcttcagca aatttaggaa aatatttatt ttcatatggg cagttaaaca
      781 tttctggcaa aactacaatt ttagctccat tgatggcagc ttctgttacc atatctacag
      841 catgattgat attttcaatt ttattttctg tagtaatcat ctgacatact gcaattttat
      901 agaaattcat agaaattact cctttcaata tttttatgtt attataacat attaaataaa
      961 aattagatat tcttagattg acctaaatgg gtatggagag aagtgaataa gaatattaaa
     1021 aggtatataa aagttatttt tattatagat ggaacccata tattgacttt ttttatatat
     1081 gttgatagta ttattataga taaaatagaa taattaatag agttttatag agttccgcaa
     1141 ttattaatga ttggtctggt ccaagataaa acccacaaca tagtttttgt tgtgaacacg
     1201 gaaggacaaa aacctgggag atattttata atatttctca ggttatttta ttggaaaaat
     1261 aagagacata tttgaaaaga gggatgaatt tatgaaatgg ttatttttgt ttgttgcagg
     1321 tttgtttgaa gtgttttggg cttttcaact aaaaaattca catggatttt ctaaattgac
     1381 tccaagtata cttactgttg ttggaatgat tgtaagtttt tattttttgt cattagcact
     1441 aaaacatctt ccacttggaa cttcatatgc aatatggact ggaattggta ccgtcggtac
     1501 agctattgta ggcatggcta ttttaaatga gcctattagt atatcaagaa ttgcatgtat
     1561 aggttttatt gtggtgggaa ttgtagggtt aaagctatta tctactaatt agagtatatt
     1621 aatttaaata agctatgaaa ttaaaggcga gaaaatgttt aaaaataatc aacatttaaa
     1681 aagtctttac ttcatagctt atcttattta taagtttatt atatttgtaa attatatgta
     1741 tatactattt atctaataaa agagcagtta aatccatagg gtcaactatt acaccatctt
     1801 tataaacacg catatttcca gatgctattt catctattaa gattacttca tcattgtaga
     1861 atccaaattc aaatttaata tcataaagct caagaccttt ttcagctata gtatcacgaa
     1921 ctatgttaga aattttttga gtcatgcttt tcattgaatc aaattgagct tgagtcataa
     1981 tgtttaaagc ttctaaacct tcagatgtta taagagggtc acaacggtca tcatctttta
     2041 aagttgcttc tacgtaacaa tctagtttag caccttcttc aacacatgaa ccataacgac
     2101 gtaagaaact acctacagct ctataacgac aaataacttc taatccatta ccaaaaactt
     2161 ttgctggtag aacttccata gttgcttctt ctaaattagc acttacataa tgagttttaa
     2221 ttccagcatt gtttagaatt tcaaagaact taacagatgt ctctaaatta gctctaccaa
     2281 ttccatctat acttagacct acactatttt cgcctgggtc aaatactcca tctttaccag
     2341 ttacatcgtc tttaaattta agtaagtaat ttccattatc taattcaaaa acatccttag
     2401 tctttccttg atacactttt ttcataaaca acctccaaaa agttaaatta ttatattgaa
     2461 taaggcatta gtgtcaaaaa agttctgata ttcgcatgaa aatttatatt gataccttat
     2521 atttaatatt atatcacaaa tagaagggaa atttccgaac aattttataa aaataacaaa
     2581 aaaagtacat tttataaaac ttataataag agaactaata taaaatgtta gtgtcgttta
     2641 tattgacaca cataagtttt aatataagaa acttttgaca cactttgaca cacatttaat
     2701 gataatttta taaaatgcat gaatacaatg cttacatata ttggcatgaa agttgcttta
     2761 gatatttaac atataaacaa tagggatata ttctcaaata aaaagtaggg gtggttaata
     2821 gtgaagtcag ttagattcta tggtataaga gacacaagag tggaggatgt ggatgtacca
     2881 aaaatattag aaaaagatga tgtaataatt aaagtaaaag tagctggaat atgtggttct
     2941 gatatatcaa aatattcaaa gacaggtcca catatggttg gagaaatttt agggcatgaa
     3001 ttttctggag aagtggcaca ggttggtaaa gaagtaagga gttttaaaat aggagataga
     3061 gttgctgttt gtccagctat gccatgtttt gaatgtgatg aatgtaaaaa aggcttgtat
     3121 tcaagatgta ataatgtagc aataatagga aataaagaac ttggaggttg ttttgcagag
     3181 tatacaaagg tgaaagagag aaatttaata aaaataccag atgagataag ttatgagact
     3241 gccgctgcac tagaaccagt atgtatagca ggacatggtc tttttaggtc agaagctaaa
     3301 gttggagata ctgttgtagt acttggtaca ggtccaatag gattattttc aatacaatgg
     3361 gcaaaaatat ttggttctac aaaagtaata gctgtggatg tatttgatga gaagttagac
     3421 ttggcaaaag agttaggggc agatatttgt ataaatgcaa aagaaaagaa tattgtagaa
     3481 gaaataaaaa ggttaactga tggagatggt gcagatattg ttattgaatc ggctggtact
     3541 ccactgacat gtggtcaagt actgttgcta gctaaaaagg gaggtacagt attgtatgca
     3601 ggtgtaccat atggggatgt agctttgact agagagcaat ttgaaaagat agtaagaagt
     3661 gaattaactg taaagggaac ttggtttgga aactcctttc cattcccagg aaaagaatgg
     3721 agtgctggat tgtatcatat gcaaaagggt gatatgaatg ttgaaaaatt agtaacacat
     3781 agaataaatt tagaagaagt accagcttat tttgaaaaag tatacaaaag ggatattttc
     3841 tttggtaaga taatgattaa tatagataat tagattagag tactaaataa taatccatat
     3901 aaaaaaggtt aagaaaggta tgattcatat ggcaggaata attatagcca cacatggaaa
     3961 tatggctaaa gcaatgttag aaagtgctga attaatagta ggcaaacagg aaaatgtaga
     4021 aactttagga ttaaaccatg gtgacagcat agatgagttt agtataaagt tagaagatgg
     4081 aattgagaaa tataaggatg aaggggtttt agtattgctt gatttttatg gcggaactcc
     4141 atttaataca tctgctatta taataaataa atttgctaag gtatgtgagt tagaatgttt
     4201 gacaggtgta aatttaccta tgcttttaga gttattttta aacagagata ctatgaagtt
     4261 aaaagacttg aagaatttat gtgaggaagt tgggatatca ggaatcaaag atgttaaaac
     4321 tgttttaaag ttgtaaaata aattaaaaat ctggaggaat aattatgagt aatatagcat
     4381 taacaagaat tgatgataga ttaatacatg ggcaagttat tactgcttgg tgtaagatta
     4441 catcggcaaa aaggatagta atcgtggatg atttagtagt aaaggaccca tttatagttc
     4501 aagttttaca aatggcagct ccatcaactg ttaaggttga agtacatgat gtagaatcag
     4561 gtgctgaagt cttaaaatca cataatggag atgaaaattt aatagtattg gtcaaatacc
     4621 ctaaaactgt cttaggttta gtaaatagcg gggtagattt aaaggaattg aatgttggag
     4681 gtatgggagc agggcttggt agaaaaagtt tttataaaaa tatatctgtg tcagatgaag
     4741 aaaaagaaat atttagagag ctaatatcaa aagatgttaa atgctttata caaatagtac
     4801 cagatgcaaa gaaaattgat gtaggaacat tattgtaaag aagactatct tgtaaatctt
     4861 taaaagaaaa tagggaggaa gaatatgcac atttcactaa ttcaagcagt attaatagca
     4921 attttttatt atctttcttg gagtccgtgg ttgacttatg ttggattttt tacatggaat
     4981 agacctctat tggctggatt tgtaacggga attatattag gagaccctgt tcaaggagca
     5041 attataggag caggtataaa tatgatatat ctaggtttta tatcagcagg gggagctcag
     5101 atgggggacc cagcttttgc aggatatgta ggtactgctt tagctatagc atctaagcta
     5161 gatgtaagta ctgcaatggc aatagctgta ccattgggaa ctgttgcaac tgtactttgg
     5221 attggaaaga tgacagtaaa ttcttttttt gcacattggg ctgatagaga ggttcaaaaa
     5281 gggaatgttg atagagtagc atttataaat atagtaccac cacagatact gttatttgca
     5341 atgagtttta taccagcatt attagttgtt tattttggtc caaatgcgat agatggtatg
     5401 ttggaagtaa tgaatgataa tgtgttacat gtatttaatg taattggggc tatgttgcca
     5461 gcacttggga ttgctatgaa tttaaaattg attggaaaca agtttactat gccctttttc
     5521 atattaggaa tactaatggc tgtttatttt aaagtagata taatagtaat atctgttatt
     5581 ggagtaatac ttgctcttac aattacttca attaagtacg gaaaagatag tgctacatct
     5641 taaatacatg tgagggggaa aatattatgt cagatataaa aaatgaagta tctaagaaaa
     5701 cgttaagcaa gaaagatgta ataaaatcat ggttaagatg gtttttcttt gcacaatcta
     5761 attacaatta tgaaagattg caatcaacag ctttttcaca ttcaatgttg ccagtactta
     5821 aaaaattata tccagataag gaagagttaa aacaagaagt ggagactcat ctagcatttt
     5881 ttaacacaga acctatatgt ggatgtgtta tacatggaat aacaatagct atggaagaag
     5941 aaaaagctaa tggagcagat gaaataagtg gagatggtat gaatgctatc aaaacaggct
     6001 tgatgggtcc actagcggga attggcgata ctttaactca aggagttata actcctatag
     6061 tgttggctgt ttgtattggt ttaactgagg gaggagcatc aattgcagga cctatattat
     6121 ttgtaatagc tcaatatata ataatgactt caatttcttt tggaatgtgg acaaatggat
     6181 ataagtatgg gaaaaaagct gttgaaagta tacttcaagg tggaatagta aataaggtta
     6241 tagaaggggc ttctatacta ggtaccttag tcatgggggg acttgtaggt aggtttatta
     6301 atttatctac accaataagt tatactagtg gtgattttaa atttagtctt caaacggatt
     6361 tacttgataa gatatttcct gggttaattc ctttggtact tactttatta gtactctttg
     6421 ctttaaaaaa aggtctttct ccaataaaaa taatgtgtat actgatagta gtgggagcta
     6481 ttacaggtat tttaggacta ttttaaataa atatatattg gtgtaagtta ggattctata
     6541 tctattgatt atatttgtag gatgttggaa aatttatatt aaaaagtttg gcaattaggg
     6601 taattttata atagattaaa ttttaaactt atgtaagcta tatgtagtat ttaatatttt
     6661 aaaaagtatc tcagaaagat ttttaatcag aatgaggtgc tttttcattt ttatataaaa
     6721 tattaagtaa tttcaaaaaa atataaattt atttaattta attaaaatat tataatatat
     6781 tagttacata ttaaaatata taatcgagat attgttaaga cattgtcgag atattataat
     6841 atataactaa tgtattataa ataatagttg taaacttcct gtgccatata ttttaaatgc
     6901 ttattcttaa atttttgcat tatttattaa aatttattat gctttaacgc taaattgagt
     6961 ttgataatta taaaacaaat aatcaaaaat agtgtaaaaa ttaatacttt ttgttaatat
     7021 taatagataa agataatcta aggtggtgat taaactgaca atagaagaat acaaaaacaa
     7081 atactttact ggaaataaaa tcgtactttc tataaatagt tttgataata aagaactata
     7141 ctatcatgaa actgaaaata tattaaattt aataaatgaa atagataaat taacatcaag
     7201 taagatattt aaaacaatag aagataaaaa tgatttggca ctagagtctt attattctag
     7261 tgtaatagaa ggtgcttttt caactagaaa gattgcaaaa tctataatta gaggaaagat
     7321 gaaaccaagt aataaaagtg aatatatgat ttacaataat catagagcct tagaatatgg
     7381 attagacaac ttagataaat tatatagtca caaatttata tatgatttgc atcatatatt
     7441 gggcgaaaac tgcttggatt cagaagaata tgagtataga actgaaaaag tatatgtatg
     7501 tgattcaaaa ggtgagatta tacatacagg gcttgaacct ttgaagatat atgactttat
     7561 gagtaaatta atagatttta tggaaaatag caaagtaagt aatttaatta aaagtgctat
     7621 aattcatttt tactttgtat atgttcatcc ttttagtgat ggtaatggaa gaacatcaag
     7681 agccttatca tatctatatt tgattgataa aggatatgat actttcaagg atttttcaat
     7741 atcatatatg atatctaaaa acagaactaa gtattataag gctatacttg atgtagaaaa
     7801 caaaggaaat aatttaactg tatttataga atttatgctc aagtctataa tacaatctat
     7861 taatgagatg cgtaatatgc atgatagaaa atctttagaa agtatattaa aagaagaatt
     7921 gtttgaaaat gacataactc tatcagcaac agaagaacat atattaaaat atatttgcaa
     7981 taaagataac tattctatga cattagaaaa ttatataaaa aagaataaaa gtagatactt
     8041 aaaggctgga ataaaggaaa ttgaattagt tgaccagttg atggaagtgt ttaataatct
     8101 tgaagagata gagattttgt ctaaagaaaa aaatatatat aaagttaatg ataagtactt
     8161 aaaaatgctt gatatagact aatttagata atctggagag ggaatatatg aataataaga
     8221 aaaaggcatt tttgatttct ggggctataa acgtaatttc attaattatt gttttttcat
     8281 tgtttcatta taatattata gaaaatagta cacaaaatat gataataact gtaatcttaa
     8341 tttttatttt tgaaatgata aaaataaaaa tcatagagaa atactataaa ctataaacaa
     8401 gaaatatctt ttctaagagc atattgtaca atatgctctt aatttagtta tatagattta
     8461 atataaataa tgtatttttt gtagatgatt tatgtaagat ttatgaccaa tttgtgtatg
     8521 atttgtgttc tttaatcatg acaatattat attgtaaaaa ataaatttaa taaaaaatga
     8581 cttggaattt atattctagg tcttttttat ataaaaattt aataagtttc aaatgttggt
     8641 aactttgaca gtagaaaacg tctattgaga taacgaagat tctaattgag ggggagatat
     8701 attagcactt aaagtagtat atgcaacaga tataatcttt ataaaagtaa tttgaattaa
     8761 aatgtaaata taaagaataa ttaaaaaaat atcgcaatta tttgtttttc atatgga
//
LOCUS       DACXMZ010000166         8734 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.167, whole genome shotgun sequence.
ACCESSION   DACXMZ010000166 DACXMZ010000000
VERSION     DACXMZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.167"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            142..1923
                     /locus_tag="JD016_14500"
     CDS             142..1923
                     /locus_tag="JD016_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAU5000840.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRVLFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(2005..2535)
                     /gene="lepB"
                     /locus_tag="JD016_14505"
     CDS             complement(2005..2535)
                     /gene="lepB"
                     /locus_tag="JD016_14505"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAU5000841.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(2575..3399)
                     /locus_tag="JD016_14510"
     CDS             complement(2575..3399)
                     /locus_tag="JD016_14510"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAU5000842.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3656..4564
                     /locus_tag="JD016_14515"
     CDS             3656..4564
                     /locus_tag="JD016_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAU5000843.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            4708..6153
                     /locus_tag="JD016_14520"
     CDS             4708..6153
                     /locus_tag="JD016_14520"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAU5000844.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            6464..6916
                     /locus_tag="JD016_14525"
     CDS             6464..6916
                     /locus_tag="JD016_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAU5000845.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(7251..8447)
                     /locus_tag="JD016_14530"
     CDS             complement(7251..8447)
                     /locus_tag="JD016_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAU5000846.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
BASE COUNT         3456 a         1112 c         1266 g         2900 t
ORIGIN      
        1 acttattgca aaattatttt agcagttttg tatttatttg tatttatttt gaatttattt
       61 actaaatttg cctataaaaa gtatatataa aaatatagtt caaaatggta taattaagta
      121 taacagaagg aggtaatgta agtgagccaa tacataaata taaattctca gttagacgcc
      181 ctaaaattga aggagttcga gaaaatattt tacgaaaata tcaatgagga cgttaaagta
      241 gtgccaaaag ttactccttt taaaggaata aataccgatt tgttgtatgt taaagatgga
      301 agagttttat ttataaaatt tatggataca acagaagata tattttttat tttagaagaa
      361 gagttacttg aagttatgaa tgaggaatat gaacttttga aactaaaaat gggacaaaag
      421 aatagaaata taagttataa ctacgtttac attatgccat acgttgaagt tgaggaaaca
      481 tatgaatttg aagaatttgt taataataat ataatagata aaaataagct tcaagatata
      541 atgaataagg gttcgctgga ggaatatctt aatgatgaga atgatgaaat aaacttaaat
      601 ttatttttac tagacatatg ttcagaatac tatataataa atgataaact tcatctaaat
      661 gaaaaattta aaaaaatttc attctacaat gatgactata agtatacagc aactatgatg
      721 gaagacgttc aaattaaaga tgttgtttct attaaatatg ggaatacact tattgaaggt
      781 ggttctggta taggcaaaac agcaattatg ctttctaggg ctataaaatt agcaaaagta
      841 tatccacatc ataaattagt catatttaca catacaaaac aattgtgcaa tgagcttcga
      901 gagaggatag agttattata caaagataat aacaatttag aagttcacac atttagttct
      961 tttatattta aactagctaa aaaattcaat ttaattgtag attataacat gttaaaaaat
     1021 gattacgaaa agacctttaa taatcttgta aaacaagcac agaacattat aaaaaataaa
     1081 aatatgttca aggctatttt tattgatgaa gcagaaagct ttttagagta tgaaatagat
     1141 tttataaggg agtttttata taaaacaaaa ttcatattta atgtgtgttc ttgtaactcc
     1201 ttgaatatct ctaatagatt gaatatattt aaaaaattat ataatggtat agaatttgat
     1261 gataagatta tcttaagtaa aaattataga caagctaaag agattgtaga ttttacaaat
     1321 aaattttcaa ataattctaa ctcatatatc aacgaattaa gaccaaatac agaatttagt
     1381 actttctttt atactaaagc acttagaggt ggaaataagt ctgttgatat aataaaagta
     1441 agtgaccttg atgaccaaat aagttctgta atctgggaaa ttgagtattt aataagtaaa
     1501 aaaggtcttg attattctga agttgctatc gtttatcctt acaataagaa gaaattaaaa
     1561 agtggaaaaa ccatttactt tcaatatatg ttaaaaaaag cattggaaga ggcaaaaatt
     1621 ccttatatat gtgcagaaga taatttgaca aatataagta agaaggtagg aactacaata
     1681 gcaaatatat atgcaataaa gaatttagag tataaagcgg taatagtatg tgaattagag
     1741 atgttataca atcaaactat aaatgatatt gaacaagatt atcaagtaaa tgactttgta
     1801 ggagatttaa ataaggttta tttagctatg agtagagcaa cagactacct aagtatagta
     1861 acaactttta atgaagaagc aagtgatata ataagactta ttacagaatc aaaagatatt
     1921 tagtaaaaaa atatttgtaa tttaagaaaa taaaaagaga ctgagatttg taaaataaac
     1981 tttaccaatt cagtctctta ttttttagtc tactttccca atattatcaa gaggtaatag
     2041 tctcaccata accttaccaa gaacttcatc ttcatcaacc atacctacat cagggaatct
     2101 actatcatta ctattttctc tattatctcc cattgcaaat agtttacctt ttggaacaac
     2161 agtatctata tctccagaag tatagttatt gtgtatatat ggttcattta ataattttcc
     2221 atttacatac actttagaat ttgatatttt tattctgtca ccttcagtag ctataactct
     2281 ttttattaag tcttttttct ttcctgtttc tccatcaact aaattggttt taaaaactac
     2341 aatatcgcct ttttcaggtt taccaacctt gtatgatatc ctatttaaaa tcagataatc
     2401 tttatcatct aaagtaggat acattgattc tccacttaca atagaaggtc ttataaattg
     2461 tagaattaca aatgcaagta caatagccat agctattgac ttaatccaat caaatatttc
     2521 ttttttaaca ctcataactt ttctccttat catttgttat taaattagaa tacattataa
     2581 tagaaagaaa taagcaagaa ccactatacc caagactatt ctataataac caaatacttt
     2641 gaagtcatgt tttttgatat agtccataaa aaatttaata actacaattg aaacaacaaa
     2701 tgctactaca gaaccaacac ctaaaataag ccattcaaat ccagtaaatc caaatccagc
     2761 ttttagaagt tttaatgcac tagctccaag cattgtaggg atggctaaga agaatgaaaa
     2821 ctctgctgct acatatctag aagtaccaag taatactgca cctattatag tagaaccaga
     2881 cctagatgta cctggtataa gagctagaca ctggaataat cctatacaaa gagctaattt
     2941 atatgtaact tgagaaaaat ctttatactt aggtttttta tttctctttt caagccctat
     3001 cattattata ccatatacaa taagagcaat agcaactact gttgaattaa agaataatct
     3061 atctatatca tcttcaaaca gtattccaat aactccagat ggtataactg caattataac
     3121 cttaagccat aaacggactg tttctttttt ctgcttaata ttctttgcgc tatcaaaagg
     3181 atttaatttt ctaaaaaata taacaagtac agctaatatt gaaccaaatt gaattacaac
     3241 taaaaatgta cttataaatg tatttgaaaa attcaatttt ataaactcat caactaatat
     3301 catatgccct gtactactta ctggtagcca ttctgttatt ccctgaacta tgcccaaaat
     3361 gactgcctta aaaatttcaa gcaaactaaa tgtttccaaa tcaaaatacc tccaatacaa
     3421 atttatagta gcatatttta ttcatagatt aataaaaaaa tgccaccttt tactaactta
     3481 taataccata gtactgtgta gtgcacaatg gaagattgat attttcataa aaccttcata
     3541 atataacaaa ataaacttaa aagttatcta ttgaataagg ggtatatttg agggggaaat
     3601 atatggtata gtaaaagtat agctatttta ttttatgaaa aggaaaggag ttattatgta
     3661 tagtacaaaa atactagatg attatgagta tgaaactttt aatgtttttt ctacaaaatt
     3721 aaatccaaac acgaaacacg attatttatc aaaggttata ctgtttaaag aatttttaaa
     3781 agggaaagaa ttgatttatg ctacgaaaga agattgtaaa aattttgtag actatataca
     3841 gactaaatat gcgaaatcaa cttgtgaaaa gatatatagt tatttacata gtttttataa
     3901 cttcttaaaa aaagaaggat acatagatat aaatccattt agatatgtag aaaaacccac
     3961 agtaacaaga ataaaaacaa aagatgatgt tctaagtata caagaaataa ataaacttat
     4021 aggtatccta ccaaaattaa atataagaga tagagtaata atagtctgtc tagttactac
     4081 tgggtgttta ttaaatgaat tagttagctt aaagtggaaa gaccttatgg tagatgaaaa
     4141 cgataactca tatgtaaggt taggtaaggg aagaaaagaa agagtagtta aattacatcc
     4201 atactttttt aaacttttag aagattacag gaattattct gggcttccag aggtaataat
     4261 acctagtgat gattttattt ttacaactca aaagagtaat tctataacag atagaaatgt
     4321 cagacttata gtcaaaaaag cattagattt agctggactt tctcaatatt cagcaagaga
     4381 ttttagacat tcatttgcag caataagttt aaggcttggg gcagatgaaa gtgatgtaaa
     4441 aaatcagctt ggatggagtg ataaatacta tgcaattaga tataagtatg tattaaattt
     4501 tgttgatagt gaaatagtgg attacttaat tgaaaaagat aatttggata taaataaaaa
     4561 atagtaataa ataaaaaata ctatatataa atgtgaacaa aaaataaaaa agtcttgtta
     4621 tagttcgtaa aattatatat aattatttat gtactaatgt gtacacagta atcacacgac
     4681 aaaagattta aggaaggtgc taatataatg atagataata aatatactac ttactcaaac
     4741 ccacttacag acagatattg tagcaaggac atgagttata tattttctcc acaatttaaa
     4801 ttctctacat ggagaaggtt atgggttgca cttgcagaag gtgaacatga gttaggaatc
     4861 aatataactg aagaacaatt agatgagcta agagctaata tagataatat aaattttgat
     4921 gaagctagaa aaatagaaaa agaagtaaga catgatgtaa tgtctcacgt aaaagcttat
     4981 ggtttacaat gtgaaaaagc caaaggaata atacatttag gagcaacatc tgcatatgtt
     5041 ggagataata cagatgttat acaaatgaat gaagctttaa aacttctaag agtaaagctt
     5101 gtaaatttaa taaacaattt aaaagaattt gcacttaagt acaaagatat gccagctctt
     5161 ggatttacac acttccaagc tgctcaactt acaacagtag gaaaaagagc ttgtttatgg
     5221 gctcaagatt taattcttga tttggaagat ttaaactata gaatagacaa catgaagtta
     5281 agaggagtta aaggtactac tggtactcaa gcaagttttt taagcttatt tgaaggagac
     5341 cacgaaaaag taaaagagtt agataagaaa atttgtaaaa agatgggata ccattcttcc
     5401 tatgcagtaa gtggacagac atatactaga aagttagatt atcaagtaat aagcatacta
     5461 tcagggatag ctcaaagtat gcataagatg actaatgata taagactttt acaaagttta
     5521 aaagaattag aagagccatt tgaaaaaaat caaataggtt catcagcaat ggcttataag
     5581 aggaatccaa tgagaagtga gagaatagca tctttatcta agtatataat ctctgaatct
     5641 ataagccctg caatggtaca agccactcaa tggttagaga gaacattaga tgactcagcc
     5701 aataagagat tggcaatacc tcaagcattt atggcagcag atgctatact tgaaataggg
     5761 ataaatgtaa ctgatggttt agtagtatat gaaaatatga taaatagacg tgtaaatgaa
     5821 gaacttcctt ttatggctac agaaacaata cttatggaag ctgtaaaaag aggtggagat
     5881 agacaggaat tacatgaaat aataagagaa tattctatga aagctgccta tagagtaaag
     5941 catgaaggca aggataataa cttaattgaa cttataatga atgatgattc atttaaaatg
     6001 agcaaggaag aaatactatc tataatggac ccaaaaaact ttataggtag agctccagaa
     6061 caagttgtag aatttgtaaa tgaagttgta gaacctgcta taaaagatta taaggaagac
     6121 ttaggaaaag tagatgtaga cctaagagta tagaattgta cagataaaat ataattaaaa
     6181 acggctatct tatttataaa taagatagcc attttaatgg gctagcgtct tctctccacc
     6241 gctgtgaaga gttcttcttt acttactata ccatgaattt aataaaatag ctatataaat
     6301 aatatttaat aggaataata taaataaaaa taaaaaaatg ataagattac taattaaatt
     6361 taaacattca gcaaaaacta ttgtacttgc aatattttta atgtaaactg aaataaagtt
     6421 atttagataa tttaataaat tttaaaacaa ccgaggtgat tatatgaact atatagtaaa
     6481 agataccata agttttttag taaaaacatt tcctaagatt tattctagcc tgtacttaga
     6541 agacctaaaa aaatttgcac cagattataa tgtaaataaa acacaattaa gagcattagt
     6601 tttcataaaa aattatggag taataagtat gactgattta tgctctaagt taaatataga
     6661 aaaaggcagt ttaactagta tggttgatga tttaactgat aagaagtatg taacaaggaa
     6721 aagagattta gttgatagaa gaaaatatct aattgacatt actgaagagg gagataagat
     6781 agcaactgat tttatggata aattaagtga tggtttagga gaaaaacttt ctaaattaac
     6841 tgaagaagac agaaaaaaat atttagaagc aataaatact cttcaatata tattaaacaa
     6901 agaagaattt agatagaaaa aatataaaaa tttttgtatt taatggtact aagattaatt
     6961 ctttgttatt catttaacta tataattata agtctaaata caatctaaaa agtacatctt
     7021 aaattttctt gatgtacttt tttataattt ctctaactaa ggcaagacaa gttttagatg
     7081 ctttaattaa agtgaataaa gattactggc caacactata taaagaaaat gaaaaatagt
     7141 caggtctaag tcttaattaa tatattaatt aagaaatgtc cccttttcaa ataatatctg
     7201 aaaaggggac attttatatc attaaattta tataaattaa gtttaaaaaa ttatttttgt
     7261 aaagctacat attgctctaa accttctttt aataatgtag cagctctatg aaggtctttt
     7321 tcatttaaga tgtaagctaa tcttatttca tcacgtccta aacctggagt agcatagaat
     7381 ccttcagcag ggcaaaccat aactgtttca ttatctttgt taaattcttt taacatccaa
     7441 attgtgaaat cttctgcatt ttcaactggt aatttagcaa ctatatagaa agcaccagtt
     7501 ggttttttac atataacacc ttcaaccttc attaattcat tgtataatac atctcttctt
     7561 tttttgtact cttcattaac ttctttgaag taagaaacag gagttttgta taactcaaca
     7621 gctcctattt gctctaatgt aggaacacaa agtcttcctt gacataattt aagtatttga
     7681 gcgataagac ctttattttt gcaagcgata gaaccaattc tagcaccaca agcactgtat
     7741 ctcttagaaa cactgtctat tattataact ctatcctgta caccctctaa gttaccacag
     7801 cttgtatact caagcccatc ataaacaaat tctctataaa cttcatctgc tattatccat
     7861 aaatcttttt ctttagctat ttcagctagg atattaagtt cttcttttgt ataaattgca
     7921 ccagttggat ttcctgggtt agaaagaatt atagccttag ttttatcatc tactttagac
     7981 aatatttctt ctttagatgg aaggtggaaa ccgttttctg cctttgttgt aacagcatta
     8041 acttctacat ttacagattg accaaatcca ttgtagtttg tatagaatgg ttctggaact
     8101 aatagattat ctcctgggtc acaaactgcc atcatagtga aaagtaaagc ttcactacca
     8161 ccattagtta ctaataattc atctttttca aaattcatat cgtaagtttt atagtaattt
     8221 tgaagtgctt ctaataattc tggaatacct tctgatgtag catattctaa gacttcgcta
     8281 tcaaagtttt taacagcatc aaaaaatcct ttaggagttt ttatgtctgg ttgtcctatg
     8341 tttaagtgat atactttgat tcctttatct tttgctgctt gtgcaaatgg aacaagtttt
     8401 ctgattggag aggcttgcat tgcactaact ctgtttgaat aattcatttt aataccccct
     8461 atttaattta aatacgtata ttgtattatg taaattgtat gtttgaatac aagacatctt
     8521 tacaagataa taataacata cttatgggta aaaatcatca aaaaaaatta atatagcttt
     8581 aaaataaact tttaacaaat tatagtaaaa tacccatgtt tatgccacta aattatgtat
     8641 tcataaatta atttatagta agataatatt ctgatatact gatagtgatt ttttagttat
     8701 aatctttcag ctatagacaa aatatataat aata
//
LOCUS       DACXMZ010000167         8730 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.168, whole genome shotgun sequence.
ACCESSION   DACXMZ010000167 DACXMZ010000000
VERSION     DACXMZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.168"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            51..1547
                     /locus_tag="JD016_14535"
     CDS             51..1547
                     /locus_tag="JD016_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA dehydratase"
                     /protein_id="HAU5000847.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            1682..1957
                     /locus_tag="JD016_14540"
     CDS             1682..1957
                     /locus_tag="JD016_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000848.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            1981..3288
                     /locus_tag="JD016_14545"
     CDS             1981..3288
                     /locus_tag="JD016_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HAU5000849.1"
                     /translation="MSWQELYQSKLCSAIEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLIDIAHPSVRESLAVEFEKRFKEKY"
     gene            3308..4426
                     /locus_tag="JD016_14550"
     CDS             3308..4426
                     /locus_tag="JD016_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HAU5000850.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            4713..5633
                     /locus_tag="JD016_14555"
     CDS             4713..5633
                     /locus_tag="JD016_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000851.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            5748..6905
                     /locus_tag="JD016_14560"
     CDS             5748..6905
                     /locus_tag="JD016_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAU5000852.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(6990..8489)
                     /locus_tag="JD016_14565"
     CDS             complement(6990..8489)
                     /locus_tag="JD016_14565"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAU5000853.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         3364 a         1094 c         1571 g         2701 t
ORIGIN      
        1 taatttaaaa taaataaaaa aatctataaa agagaaggag tggttttatt atggcattaa
       61 tgacaggagc tcaatatatt gaaagtttga gaaaattaaa tactaaggtt tatatgtttg
      121 gtgaagaggt taagaattgg gttgaccatc ctatgattag accatctata aattgtgtag
      181 cagcaactta tgacttagct catgacccag aatatgcaga tttaatgaca gttacttcaa
      241 atatcacagg tgaaaaaata aatagatttg gtcatcttca tcaaagtgtt gatgatttga
      301 taaaaaaagt aaaaatgcaa agactatgtg gtcaaaaaac agcttcttgt ttccaaagat
      361 gtgttggtat ggatgcgttt aatgcagtgt attcaacaac atttgagtgt gataaagccc
      421 atggaactaa ttatcatgat aattttgtaa aatacttaac ttatatacaa gaaaacgatt
      481 tagtagttga tggagctatg actgacccta agggagatag aagtctatca cctagtgctc
      541 aacctgaccc agatatgttc cttcatatag tagaaagaag agaagatggg ataattgtaa
      601 gaggagcaaa ggcacatcaa actggttcaa taaattcaca tgagcacctt ataatgccta
      661 ctataagtat gactgaagca gataaagact acgctgtatc atttgcagtt ccttcagatg
      721 ctgaaggagt gtttatgatt tatggaagac aatcttgtga cactagaaaa ttagaagaag
      781 gtgcagatgt tgatttagga aataaggagt ttggaggtca agaagcttta gtagtatttg
      841 ataatgtatt tgttccaaat gatagaattt tcttatgtgg agaatgggat ttctctggta
      901 tgttagtaga gagatttgca ggataccata gacaaagtta tggtggatgt aaagtaggag
      961 taggtgatgt aataatagga gcagcagctt tagcagctga ttataatgga gcaaacaaag
     1021 cttcacatat aaaagataaa ctaatagaaa tgactcacct taatgaaagc ttatattgct
     1081 gtggtatagc atgttcatca gaaggtcaca aaacagaagc tggaaactat caaatagatt
     1141 tacttcttgc aaatgtgtgt aaacaaaatg taactagatt tccttatgaa atagtaagac
     1201 ttgcagaaga tatagctgga gggcttatgg ttactatgcc ttcagaaaaa gactttaaat
     1261 cagaccttaa agttggaaca agtggaatga ctattggcga agtatgtaac aagtatttta
     1321 aagcttcttc agtggcttca acagaagaaa gaatgagaat attaagattc ttagaaaata
     1381 tatgccttgg ttcatctgct gttggttata gaactgagtc aatgcatggg gcaggttcac
     1441 cacaagctca aagaataatg atttcaagac aaggaaacat aaatcaaaag aaagaattag
     1501 ctaagaaaat tgcgggaata aaaaaagagg aagctttaaa tttataaaaa gttgatatat
     1561 aaagggaaat tatatattaa tgtgtattaa ttaatgaaaa aatatgtagt ataagagata
     1621 gttataaaat tatggtggtt tttagctaag taaagaaatt tttaataagg agaatttaca
     1681 catgacctgt ggagttagat tttgtggggg ctgtaaccct agatacgaaa gagggaaggc
     1741 tcttacaata atcaaagagc acttcaatgg tagggtagat tttagtatag cacaagagaa
     1801 cgtcgagtat gatttgctac ttataattgg aggatgcgca actctatgcg catcctatca
     1861 acaatataaa gtgaaaaatg aatgtgttaa gatgtgggat tttagccata tagaaagagt
     1921 tataaatgtg attgaatcag caatttgtga aacttaaaaa agctaaatgg ggggatttca
     1981 ttgagttggc aagaattgta tcaaagtaaa ttatgtagtg ctatagaagc tgtaaaacag
     2041 attaaaaacg gtgatactgt agtatttgca cattgtgtag gtgaacctcc tgcactagta
     2101 gaggctatga ttgaaaatgc tgaacaatat aaagatgttg agattaaaca tatggtaagc
     2161 ttgggtagtg gtggatatac tgctaaggga atggaagctc attttagagt aaatccaatg
     2221 tttgtaagtg gaaatgtaag aaaggcaatt gaaaatggag atggtgattt tacacctgca
     2281 ttcttccatg aagtaccaaa gttgcttaga gaaaaaagat taaaatgtga tgttgtttta
     2341 gcacaggtaa ccccaccaga tgaacatggg tattgtagct taggaacatc agttgattat
     2401 acttatgaag ccattaaaac tgctagaaca gtaattgttc aagtgaatga ccagtttcct
     2461 agaacctatg gtgaggtagt ccatgtatca gagtttgact atatagtgga aaaatcacaa
     2521 cctttatttg aactacaacc tgcaaagatt ggagaagtag aagaagcaat tggtaaaaat
     2581 tgtgcttcat taattgaaga tggttctact ttacagctag ggattggtgg gataccagat
     2641 gcagttatgt tatttttaac tgataaaaag gatttaggga ttcattcaga aatgatttca
     2701 gatgggactt tggcccttta tgaaaaaggt gttataaatg gtaaatataa aaattttgat
     2761 aaagaaaaaa tgacagttac cttcttaatg ggtactaaaa aattatatga ctttgcaaat
     2821 aataaccctg cagtagaggt aaaaccagta gactatgtga atcatccagc aattataatg
     2881 aaacaacata agatggtttc tataaattct gctatacaag ttgatttaat gggacaggta
     2941 gttgcagagg ctatgggact tagacaattt agtggtgttg gaggtcaagt tgactttata
     3001 cgtggagtgt ctatgggtga agatggaaag gcaattatag ctatgccttc aataactaca
     3061 aaaaaagatg gtacagtaat ttcaaaaatc gtatctattg tggatgaagg tgcacctata
     3121 acgacatcaa gaaatgatgt tgactatatt gtaacagaat acggtatagc agaattaaaa
     3181 ggaaaatctc ttagagaaag agcaagaaat ttaatagata tagctcatcc aagtgtaaga
     3241 gaaagtctag cagtagaatt tgaaaagaga tttaaggaga aatattaata atagaggggt
     3301 gttaataatg gaagcactta gagtagttcc aaagatattt tactttgata catttaaaga
     3361 atttaatgaa gaatttaaaa taggaaaaaa tgatttagta attactaatg agtttatata
     3421 tgagccatat atgaaacctc ttggaataga tacaaattta atttttcaag aaaagtttgg
     3481 aacaggtgaa ccatctgatg aaatgataga ctccatgaca aaggaaatga agaaatacaa
     3541 ttttgataga attattgcat ttggaggagg aacaatagtt gatatatgta agatattagc
     3601 acttgatgtt ccagaaaagt ctattgattt atttgaagga gaagtatcac ctaaaaaagt
     3661 aaaagagtta gtagtagttc ctacaacatg tggtacaggt agtgaagtaa caaatgtagc
     3721 tattgcagag ttaaaatcta aacatactaa aaaggggctt gcagtagaag aaacatatgc
     3781 agactatgca gtgttaattc cagagacagt aaaagggtta ccttacaaat tctttgttac
     3841 aagttcggta gatgctctta tacatgctat tgagtcttat ctttctccaa aagcaagtcc
     3901 atttacagag atgtattctc tacaagcaat aaaaatgatt atggatgggt ataagaagat
     3961 agttgataaa ggagaagaag agagatttaa tcatcttaga gactttgtac ttgcatcaaa
     4021 ttatgcagga attgcttttg gaaatgctgg ttgtgcagca gttcatgcac tatcttattc
     4081 aataggtgga gctttccatg ttgcacatgg tgaagcaaat tatcagttct ttactgaagt
     4141 atttaagatg tattctagaa aaaaacctaa tggaaaaatt ataaaatgta caaaaatttt
     4201 agcagatgcc cttgaatgtg accctaactg tgatgtttat ggagaattag aaaagttttt
     4261 aaataaatta atagctaaaa aagctttgag agaatatgga atggttgaaa gtcagataga
     4321 tgaatttaca gatagtacaa tagcaaatca acaacgacta cttgctaata attatgtaga
     4381 gcttagtaga gaggaaataa gagagatatt tgcaaatctt tattaataca attttttatt
     4441 tatatgtata gtcaacataa acaatgctaa ttatatttat gtacaaataa aaataaggga
     4501 acttaaattt taattaaagt taagttctct tatttttata tggagttaaa tataagtata
     4561 tttttaattt aaaatatgct tatatttaag tctattactt tatttgaaac aatttgtaga
     4621 atttcaaatt ccttgacagt aataagtaag taattgtata atcaaactgc aatatattaa
     4681 taatattagt gaaaatgaaa ggattataaa atatgtataa atcagaaaat ttttcaaaaa
     4741 aatctgtatt aataaattca tttttaagta aatttttaaa aataattgga tggggtttgg
     4801 taagcttatt tacattatca gccttagtac tttcaactat ttccattata attggatggg
     4861 ctgtaatact tcctattcaa gtatgtgcag tatttttact tataggagga gtttgtattt
     4921 ttaaaggtaa gagattagga gaccaaattt ctcgttataa aaaatattgt acaattataa
     4981 acaatagaaa tattattcca gtagaactta ttgcagaaaa cacatctaaa tcattaaatt
     5041 ttataataaa agatgtacaa aagatgatag acaaaaacta ctttataaat acttatattg
     5101 ataaaagaaa taatcagatt gtgttaacaa atgaagattt tattccacca agatatgaag
     5161 atgtagcata tgaagtcaaa gaagaagaaa agactgatga atccaatgaa gttgatacaa
     5221 ttataaataa aggaatgaat tatctaacac aaataaaaga agctaataaa aatattaaat
     5281 cagaatcaat gggcgataaa attgtacaag tagaagatgt tacaagtaaa atatttgatg
     5341 ttgtaaaaca tgacccatca aaacttactc agatacaaaa atttatggat tattatcttc
     5401 caaccacttt aaaactatta aattcatatc atacattgga agaacaagga attgatagag
     5461 aaaatataac aacaactatg gaaagtattg aaaatacaat gaatacgatt gtagtagctt
     5521 ttgaaaatca attggactat ctgtttgaag atgaagccat tgatatttct acagatatta
     5581 ctgttttaga gaatatgtta gtccaagaag gattaacgag tggtaaattt taacttcaat
     5641 agaaaaaata ttctgttatt tttaaacaat taattagtca taaagtataa aatgtgtatt
     5701 atataagtta atcagtactt tatgaaggaa gaggagagaa agatacaatg aataatttag
     5761 atgatatacc agttatgcca actttgacac ttgacccatt tggagaagaa tcaaacacta
     5821 atgatataga taattctgac ttattaatga aaaaggatga aaaagaccca gaagaagaaa
     5881 aactgtctga atctgaacgc aagatggtaa aggaatttgc ggaaaaaata gatattacaa
     5941 atacaaatat gattttgcaa tatggagctg gtgctcaaaa aaaggtagca agtttttctg
     6001 aaacagcttt aaagagtgta aaaacaagag atttaggaga agttggagat atgcttacaa
     6061 atctagttac tgatttaaaa gcattttcag cagatgaaaa agaacaaagt ggttttttag
     6121 gaatttttaa aaaggcaaat aataaaattt caaatttaaa aactaagtat gatagtgcag
     6181 aggtaaatgt ggataaagta agcaaagagc ttcaaaagca tcaggttaaa atattgaaag
     6241 atatagcaat gcttgataag atgtatgaat tgaacttagc ttactcaaaa gagcttacta
     6301 tgtatattat agcaggaaaa caaaaattaa aggatatgaa agaatatgag atgccaaagt
     6361 tgagagaaaa agctcgttta tctggttcaa cagaagatgc tcaatctgta aatgatatgg
     6421 tttcactttg tgacagattt gaaaagaaga ttcatgactt agaattaact agaatggtat
     6481 cgcttcaaat ggctcctcag ataagattgg ttcaaaatag taataatctg atggcagaaa
     6541 agattcaatc aactatagta aataccattc cattatggaa aaatcaaata gtgcttgctc
     6601 ttggaatttc tcacttaaat caagctatga aagcacagcg tgaagttagt aatatgacaa
     6661 atgagctttt attaaagaat gcagaaactt tgaagatggg aacaatagaa acagcaaaag
     6721 aatcagaaag aggaattgta gatatagaca caattaagaa aactaatcaa tctctaatat
     6781 ctactattga tgaggtagtt aaaatacaac atgatggtag aataaagaga caagaagcag
     6841 aagttgaatt atcaaaaata gaaaatgaac ttaaaagtaa gcttcttgaa tttacagtta
     6901 aataaaaatt atactaatat aaaaagagac tgtctcaaaa tagagataag tctctttttc
     6961 attgtttaaa tgattaaagt atatttatat tataaatttc tattatcttt taacataaca
     7021 tcatgagctc caccttcaac tatactagta gaagatacaa gtgtaagttt agcatttttt
     7081 tgaagttcag gaatgctaag tgctccacag ttacacatag tagaacgaac tttacttaaa
     7141 gaaagagtta cattgtcttt caaagaacca gcataaggca cataagagtc aacaccttct
     7201 tcaaaagaaa gtttcttatc tccaccaagg tcatatcttt gccagtttct agcacgagca
     7261 gaaccttctc cccaatactc tttcatataa ctaccattta tattaacttt gttagttgga
     7321 ctctcatcaa aacgagagaa gtatctacca agcattataa aatctgcacc catagctaaa
     7381 gctaaagtta tatgatggtc ataaacgata ccaccatcag agcaaattgg aatataaata
     7441 cctgtttttt caaagtattc gtctctagct tgagcaactt caattataga agtagcttgt
     7501 ccacgtccta tacctttttg ttcacgagtt atacatatag aaccaccacc aataccaact
     7561 ttaacgaagt cagcaccagc ttcagctagg taaaggaatc cttcgcggtc aactacattt
     7621 ccagctccta ccttaactgt atcaccatat ttttcacgaa tgaaatcaag tgttattttt
     7681 tgccactcag aaaaaccttc tgaagaatct atacataaaa catcagcacc agcttcaact
     7741 aaagcaggaa cacgttcagc aaagtctcta gtattaatac cagctccaac tacatatctc
     7801 ttagaagaat ctaaaagttc aagtggattt tctttatgag aagagtaatc tttacggaat
     7861 accatataca taagattacc attatcatca agtacaggaa gagaattaag tttgtggtcc
     7921 catataatgt tgtttgcttc ctttaaagta acatctttgt tagcataaac tatagatgac
     7981 ataggagtca taaattcact aactttagta tctaaatcca tacgacttat acgataatca
     8041 cgacttgcaa caatacccaa taatttacca gttgaagttc catcttcagt aacagctact
     8101 gtagaatgtc cagttttttc ttttaagtca agtatgtctt ttaatgtgtt atcaggttta
     8161 atatttgaat cacttacaac aaaaccagct ttgtgggatt taacacgtgc taccatagca
     8221 gcttcatttt caatagtttg agaaccgtat ataaatgaaa taccaccttc tttagcaagt
     8281 gcaatagcca ttttgtcatc agaaacagat tgcataatag cagaagtcaa aggtatattc
     8341 atataaatat ctgcttcctc gcccttttta aatttagtaa caggtgtttt taaacttaca
     8401 ttagcaggta tacaatcagt agaagagaaa cctggtacaa gaaggtattc actgaaagta
     8461 tgtgatggtg tatcaaaata aaacgccatt ttttaatcac tccttatatt tttatacttt
     8521 ttataattga tagtattaat tataacttat tctttaattt tattaaagta ttttgtcaaa
     8581 tattgagatt tttgaaaaat ttaaactatt ttaattaaat ttgtttaata tatattgcct
     8641 tatagggtaa agttgtatat taaataatgt ttagtgttta atagtagcta atttaaaatt
     8701 aatctttagt gattcatata attacatttg
//
LOCUS       DACXMZ010000168         8709 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.169, whole genome shotgun sequence.
ACCESSION   DACXMZ010000168 DACXMZ010000000
VERSION     DACXMZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.169"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            199..2853
                     /gene="adhE"
                     /locus_tag="JD016_14570"
                     /gene_synonym="adhC"
     CDS             199..2853
                     /gene="adhE"
                     /locus_tag="JD016_14570"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAU5000854.1"
                     /translation="MAHKEQIIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     SIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKKFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            3129..5348
                     /locus_tag="JD016_14575"
     CDS             3129..5348
                     /locus_tag="JD016_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAU5000855.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSRPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNDGTIILHPQGDSLSKNIYKLLEQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFVAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LSEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIQMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRKHGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            5602..6264
                     /locus_tag="JD016_14580"
     CDS             5602..6264
                     /locus_tag="JD016_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000856.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            6635..8044
                     /locus_tag="JD016_14585"
     CDS             6635..8044
                     /locus_tag="JD016_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAU5000857.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKVSGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMVNLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            8159..8434
                     /locus_tag="JD016_14590"
     CDS             8159..8434
                     /locus_tag="JD016_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000858.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
BASE COUNT         3465 a          895 c         1558 g         2791 t
ORIGIN      
        1 attagtgatt tgcgtaaaaa ataaagtgat atctagttaa attaagtatt tgtaaagcta
       61 gatattatta gtagaaatga tgttatatta gataaaaaat aaaatgttaa aaaaataact
      121 tgaaaataga aaaagtaaat gctataatgt taataaatta acataataaa taaaaaacta
      181 tctcaggagg ttgttaaaat ggctcataaa gaacaaataa taatagaaaa taaaaaagaa
      241 aatgaagtta tgtcagttgg tttagatact gtaaataacg ttgaaagctt agttaaaaaa
      301 ctagcaaaaa tcagagaagc tcaaaaaata tttgctacat atagtcaaga acaggtagat
      361 aaaatttttt tagcagcatc tttggcagct aataagcaaa gagttcctct agctataatg
      421 gcacaaaaag aaactggtat gggaattgct gaagataagg ttataaagaa tcattatgcc
      481 tcagagtata tatataatgc atataaagaa actaaaactt gtggtgttat agagaaagac
      541 gaagcatttg gtatgactaa aattgccgaa ccaataggtg ttattgcagc agttgtacct
      601 acaactaatc caacttctac tgctatcttt aaagcacttt tagctttaaa aactagaaat
      661 ggaataattt tctctccaca tccaagagca aaaaattcta ctatagaggc tgcaaaagtt
      721 gttcttgagg ctgctgtttt agctggagct cctgaaggta taataggatg gatagatgag
      781 ccatctttag aattaactac aactgttatg aaagaagtgg atttgaccct tgctacagga
      841 ggtcctggaa tggttaaatc agcatattca tctggaaaac cagctattgg agttggtgca
      901 ggtaatactc cagcaataat tgatgatagt gcagatataa aaactgctgt aaactcaatt
      961 cttgtatcta agacttttga caatggtatg atatgtgctt ctgaacaatc ggttatagta
     1021 ttagaaagta tatacaatga agtcaaaaaa gaatttaaag agcgtggtgc ttatttatta
     1081 gataaagatg aaacagaaaa aataagaaat ataattttag taaatggagg attaaattct
     1141 aaaatagttg ggcaaacagc ttgtactata gcaaaacttg caggctttga agtcccagta
     1201 gatactaagg ttttaatagg tgaagtagaa tcagttgaaa tagaagaagc ttttgctcat
     1261 gagaaacttt caccagtact tgcaatgtac aaggcaagtg attttgatga tgcagttaga
     1321 aaagcagaaa aattagtaga agatggtgga tttgggcata catcttcatt atatattgat
     1381 gatgtaaatc aaagagaaaa acttgataag tttacttcag ccatgaaaac ttgtagaatt
     1441 ttaattaata ctccttcttc tcaaggtggt ataggggact tatataattt taaactagct
     1501 ccttctctta ctttaggatg tgggtcttgg ggtggaaatt cagtatctga aaatgtagga
     1561 gttaagcatt tacttaatat taaaacagta gctgagagga gagaaaatat gctttggttt
     1621 agagcaccag aaaaagttta tttcaaaaaa ggttgtttag gagtagcatt aaaagaactt
     1681 aaagatgtaa tgaataagaa gagagctttt attgtaacag atacattcct ttacaataat
     1741 ggatatacta aagcagttac tgatttatta gatgaaatga atattaagca cactacattc
     1801 tttgaagtag aaccagaccc aactttggaa tgtgctaaga taggcgctaa agctatgaga
     1861 gaatttaatc cagatgtaat aatatctata ggtggaggaa gtgcaatgga tgcaggaaag
     1921 ataatgtgga cattgtatga gcatccagat gtagatttcc aagacctagc tatgagattt
     1981 atggatataa gaaaaagggt atatacattc cctaaaatgg gtgaaaaagc taattttgta
     2041 gctataccaa catcagcagg tactggttca gaagtaacac catttgctgt tataactgac
     2101 caagatacag gagtaaaata tccattagca gattatgaac tgatgccaaa tatggctata
     2161 gttgattctg atatgatgat gaatatgcca aaaagcttaa catcggcttc tggtatagat
     2221 gctttaactc atgcattaga agcatatgta tctatgcttg caaccgatta tacaaatgga
     2281 ttagcgttac aagctattaa gagtatattt gaatatcttc cacgtgcata tgataatggt
     2341 gcaaaagacc cagaagctag agaaaaaatg gcaaatgctt ctacaatggc aggtatggct
     2401 tttgcaaatg catttttggg tgtttgtcac tctatggctc ataaattagg tgctttccac
     2461 catgtaccac atggtgttgc aaatgctctt ttgataacag aagttatgaa atttaattct
     2521 tcagatgcac caaagaaaat gggagcattc tctcaatata aatatccaga agcattaaag
     2581 agatatgctg aaatagcttc atttttagga ttaaaaggaa attctgatga agagaaattc
     2641 caaagtttat tggtagctat tgaagattta aaacttaaag ttggtattcc taaatctata
     2701 aaaaagtttg gagttgaaga atctaagttt atggattcaa tagatgaaat ggtaattcaa
     2761 gcatttgatg accaatgtac aggagcaaat ccaagatatc cacttatgaa tgaaataaaa
     2821 gatatgtatt taaattcata ttatggaaga taagtttgct ataaaataga taaaataaaa
     2881 actcttatac ataaaacaaa gttttattgt ataagagttt ttttgtttga gaaaatggat
     2941 agtttttatt ctttaattta aaattcaatt aaatagtgtt attataataa taaaagaatg
     3001 tttttatggt tattatgttg attttatgtg taacatttaa gaatagttat atacaatata
     3061 aatgatgaat tggtataaaa aggatatatt atctatatga agtattgact aattagggga
     3121 ggagtcgaat gacagggaga aataaaagtt ttacattaat tacaattctt tttttaacat
     3181 cattattttt gacaatgggg tatatctata tagaagatac aaaacatctt cttatgtcag
     3241 aggcaaaaat acatataaaa gaagttgcag tacaaggctc tcaactagca caaagacaaa
     3301 ttgaaaaaga tttagatgtt ttaaatattt tttctaacta ttatgcttca aatccagata
     3361 tacctaatga ggaaaaaatg aaaaatcttt tagatgaaat ggaaaatcaa aaattttaca
     3421 ctatggcaat agttgatata aatggtaatg ctgaaaatac caaaggtgat aagttttcag
     3481 tgaaagatag agaatttttc aaaaactcaa ttaaaggtaa gaagtatgtt tcaaggccat
     3541 atgttgatga agtaaataag tctataaaga agattgctat ttcagtacca ttattaaata
     3601 atgataaagt agttggagtt ttatattgta cttacaatat caatactctg atgaaactta
     3661 ttaatatatc tttttatgaa aataatagta tatcttatgt tgtaaaaaat gatggtacaa
     3721 ttatccttca tccacaaggg gatagcctat caaaaaatat ttataaattg ttagaacagg
     3781 ataatgatat tcaagaagta aatagattaa aaaaagaatt gcaagaaaat aaaacaggtg
     3841 caactgtatt aaatatgttg gaagaaagaa gatatttagg atatgccact atggataatg
     3901 ggaatagtga aaataattat ataaaagact ggaatttaat tttttcaatt ccagaaactg
     3961 ttgtatttag taattcaaaa caaataatca acagagctgt atatgcagtg ttatctattg
     4021 tattaatatt tgttgcaatt attttttata taatttatat aaaaaaatct aatgaaaaag
     4081 aaatattaag tttggcttat gaagataaag ttacatatat aggaaatcaa aataaatttt
     4141 atagagagtg tagtaagtat cttttagata aaccatcttt aaattatatt atagtatatt
     4201 ttgatattaa taactttaag atgattaatg atacttttgg atatgaattt ggtgacaatt
     4261 tattaattac aatagcaaaa gctttaaaag aagaattaac agaaggtgag gtatatgcac
     4321 gactttcaag tgattatttt gcaattttct gtgattataa aaatggaaga aataaaatta
     4381 taagaaaact tgataatata cgaagtaata ttgaaagtaa tttgagtata gtctttgaaa
     4441 tctctctgtg tgtgggtatt tattttgttg aagaaggtga agtagatatt caaaaggctg
     4501 taaataaagc aaatatggct aggtctgttg ctaaaggtaa aaatataaat tatgctattt
     4561 ataatgaaga tgttaggaat aagcttagtg aagaatcaat gattttagat gatataaaaa
     4621 tagcattggt aaaaaatcaa tttgaagtgt attatcaacc taagttttca cttgtaactg
     4681 gagaaatgat agggagtgaa gcactaataa gatggaatca tccagaacat ggttttataa
     4741 gtcccgcagt atttattcca atagcagaga aaagtaagtt gattttaaaa attgggagat
     4801 ttgtatttga aagggtttgc aatgatttat ctgaatggaa aaagcaaggt aaaaagatag
     4861 ttccagtttc agtaaattta tctagagtcg agttgtatca accagatatt gtaaaattta
     4921 tcaataaaac aatacaaatg tataatctaa gttcagattt cattgaaata gagataacag
     4981 aaactgtggc tattaatgag ttaaatattt taaagaatgt tttaaatgaa ttgaggaagc
     5041 atggattttc tatttctatg gatgactttg gcacaggata ttcttctatt agttgtttaa
     5101 gagatatgcc aattgatata ttgaaactag ataagtcttt tcttggtggc attgaacatg
     5161 atgagagaag tcgcaatata gcaaaatcaa tagtgtctct agcaaaatca ctagatttag
     5221 ttgtaattat agaaggtgtt gagagtaaag aacaagctga gttaatgaaa caatttggat
     5281 gtgatttagt tcaaggattt tattttgcac gaccaatgcc agctaagaat tttttagatt
     5341 tactttaaaa caaaatttga tttttatata aagcactatt atgaatgctt gattttaata
     5401 aatcattgct aaataattag tgttttttta ttttatcaat gtattgtcta atataaagca
     5461 aaaaataatt gtattttata aaaattatat taaaaataaa tttattttgc taataaacat
     5521 gttattaaca aaagaatagt actataatgt atagatgata aaaactttaa taaaaattta
     5581 gtgtatagga gttgtttaag tatgatagat gtaagtattg atgacaaact taaggaaaga
     5641 tggcctaatg ctaagctggg ttgcattcaa gcaaaagtag cagtaaataa aagctcagaa
     5701 aaattaatta atgaaataaa tgagttttgt gatactataa atcaaagtct aaaagttgaa
     5761 gacattacga aactagataa aattaaagat gcaagaaatg cttataaaga gcttggaaaa
     5821 aatccaagta gatatagaac atcttcggaa gcattggtta gaagaattat tcaagggaag
     5881 ggtctttata caataaataa tatagtagaa ataaacaatt taatatcaat aaaatcatta
     5941 tatccagtag gtacatataa tgtaagcaaa ttacattcac cagtatgttt tactgtagga
     6001 gatgaaggag aacagtataa gggcattggg aaagaattaa taaatataga aaatcttcct
     6061 atattgtctg attcagtggg taaatttggg agtccaacta gtgattctga aagagcaatg
     6121 ataacagaag atgcaaatga aatattaatt tgtatatttt cttttagtca agaatctgat
     6181 attgaagaat attttggata tgctaaggaa ttacttaaag aatatgcaga tggaaaagat
     6241 atagaaacta aaataattaa atagaatcta tataacaacg ttatgttaag ctaaaattca
     6301 atataaaaat aagttaagat aaagaaacta acacaaaatt agtttctttt ttaaaagagt
     6361 agtttagtgt tacgaaaata gatagatagt cccacgatta ttaaaagtat ggtaatatat
     6421 taatatagca gagattgtgt ttaagggatt ttgaaagggg ggatttataa taatcatttt
     6481 atctaaaaga taaaatggtt ttatggaaat aactatatat ttaaaaatat aaataatgat
     6541 acagtaattt gggtatataa aataaggcat acatggtttt gtttaaaaac caataaggta
     6601 cataggtaat tacaatgtaa ggaaggattt aattatgatt gatggaaaag aggtaaaaca
     6661 agaaagtgga caaaaaaatc taaaatttaa gatagctata gtagtaggtg ttttgtttgt
     6721 agcttatata ggaatggcaa tcttttttag aaatcatttt tactttggaa cgactattaa
     6781 tggcgtaaag gtttcaggaa aaacagttga gcaagtaaat gatttgttaa catctagtac
     6841 aaattcctat actttaaact tagaggaaag aaacaataag aaagaacaaa taaaagcaaa
     6901 agatattgat ttaaaattta caccagatga taaagttcaa aagataaaag atagtcagag
     6961 cgctattgga tggattggtg gaatatttaa gtctaaagag tataataata tggtaaatct
     7021 ttcatataat aaagatttat tagaaaaaag atttaacgct ctatcttgct ttgatagtaa
     7081 aaatgttgta gaacctaaga gtgcttatcc taagtatgat agcactaaaa acgcttatgt
     7141 tatagtacca gaggtacttg gaaacaaagt gaaaaaagat gatttttata agttagtaca
     7201 aaactcaatt aattctggga atacttctat ttctctggat gaaagtaaag tttatgaagc
     7261 accaaacaac acaaaggatt cagaaaagtt gaaacaagct attaagacat tagataagta
     7321 tgttggtgta gtcgttacat atgattttag tgaccgtaag gaaactttag atggttctga
     7381 aattaataaa tggttaaaag tagactcaga aaagaattat gacataactt ttgataatga
     7441 tgctatggta gaatatacaa gaggtttaag taaaaaatac agtacttttg gtgactcaag
     7501 accatttaaa actgccacag gtaaagatat tacaatttca ggtggaattt atggttggtt
     7561 gatagataaa aagaaagaag ctgaagcatt agttgatgtt gtaaaagctg gtaaaaatgt
     7621 tacaagagaa cctatttaca tacaaaaagc tgtatctcgt aataaagatg atattggaaa
     7681 tacatatgta gagattagtc tttctggcca acatatgtgg ttctttaaaa atggagaaat
     7741 gcttgtatca acagacgttg taacaggtga tttagctaga ggatttgcta caccatcagg
     7801 tatatatcct cttaattata aggctagaaa tgtttcatta actggtcaag gttacagttc
     7861 acctgtaagt tattggcttc catttaatgg gaacataggc attcatgatg ctacttggag
     7921 aaattctttt ggtggaagta tatataagtc ttcaggttca catggatgtg taaatacacc
     7981 tttttctaag gcaaagaaaa tatatgaaaa tatagaacca ggtacaccaa ttattttata
     8041 ctagtaaatg tgttgctaac agttaagcga agtcatctta gtctatttag gatggcttcc
     8101 tttttatata aagcttattg aagtttacag aaagactact taaaaggaga aatacataat
     8161 gaatgaaaaa ttaactaaaa ttttagaaga agctaagaat gatgatgaat ttagaaagga
     8221 acttataaaa acaagagaac aacgtgatgt tatggattca ttttgtaagg ttgttacatc
     8281 tagaggttat gatatgacag taggggaact tttttctatg ggagaagaat atacatccaa
     8341 tctactgaaa agtgtaaatg gaggagcagc atatcctatt gaagattgga gtgattggta
     8401 cgaggatttt tttataatgt tgtcgaatat atagagtaac taatgatatt atctacttaa
     8461 gattaataca ttgccttaaa ataaaactat tagaactagg tgtaagtttt atcaatcttt
     8521 aaaaatattg atttatttct aactagatat aaaaaaatta gattaaaaat aaggtgacaa
     8581 aaatcgatat agcttgatga tttataaaag ttagataaaa tagtgcgaga tatttctaaa
     8641 aattcaaaaa aaacaataaa cttcaatggt tagaggttga aaaagaatat aaaaaacttt
     8701 tatatgtaa
//
LOCUS       DACXMZ010000169         8694 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.170, whole genome shotgun sequence.
ACCESSION   DACXMZ010000169 DACXMZ010000000
VERSION     DACXMZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.170"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            234..1229
                     /locus_tag="JD016_14595"
     CDS             234..1229
                     /locus_tag="JD016_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAU5000859.1"
                     /translation="MFITVVLFLVGFLLITKGADIFINCTVEIGKKTNISEIILGATI
                     VSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICNTGLALGLVVFISPFNVDKKMFF
                     SKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIFYMVNNYRSVVGKSDTKNRNIKN
                     NTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMMIIGSQILIESGVIIASFLNIPQ
                     GIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNILGANILNIVSVIAVSAIPNNIP
                     ILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGILMLFTYFLYISILYFMYII"
     gene            1320..2933
                     /locus_tag="JD016_14600"
     CDS             1320..2933
                     /locus_tag="JD016_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAU5000860.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            3208..5193
                     /locus_tag="JD016_14605"
     CDS             3208..5193
                     /locus_tag="JD016_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAU5000861.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIK
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKVGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKVKSFDEFYKIITKRR"
     gene            5198..5632
                     /locus_tag="JD016_14610"
     CDS             5198..5632
                     /locus_tag="JD016_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAU5000862.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            5661..6221
                     /gene="srlA"
                     /locus_tag="JD016_14615"
     CDS             5661..6221
                     /gene="srlA"
                     /locus_tag="JD016_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087241.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAU5000863.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            6285..6653
                     /locus_tag="JD016_14620"
     CDS             6285..6653
                     /locus_tag="JD016_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HAU5000864.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            6678..7244
                     /locus_tag="JD016_14625"
     CDS             6678..7244
                     /locus_tag="JD016_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAU5000865.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            7285..7650
                     /locus_tag="JD016_14630"
     CDS             7285..7650
                     /locus_tag="JD016_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HAU5000866.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            7751..8524
                     /gene="srlD"
                     /locus_tag="JD016_14635"
     CDS             7751..8524
                     /gene="srlD"
                     /locus_tag="JD016_14635"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAU5000867.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
BASE COUNT         3474 a          885 c         1618 g         2717 t
ORIGIN      
        1 atcctgtata cataaaaaat aaaaaattgt cgctttaaag tataaaacag attatatgtt
       61 aataatgtgg tgaaattata cccatatata aataaaataa aacaaataaa tagctaatta
      121 aggagaaaat attttttatt ttttgtttag gataaataaa ttttattatg gaaaaaagaa
      181 aaagcataca tcttcacaaa ctgaatattt atttaatagg gggaggtgat agattgttta
      241 tcacagtggt attattttta gtaggtttcc tattaattac taaaggcgca gatatcttta
      301 taaattgtac tgtagagata ggtaaaaaaa cgaatatatc agaaattata cttggagcta
      361 ctatagttag ttttgcaaca acattacctg aatttacagt gtctctactt gcttctattg
      421 atggtcatac tactatgagt ttaggaaatg cagttggttc tataatatgt aatacagggt
      481 tagcattagg gttggttgtt tttataagtc catttaatgt agataaaaaa atgttttttt
      541 ctaagtcttt actattaata gtatccgtta tagttttaat tttattgagt ttagatggag
      601 ttataacaag aggtgattca ttattactta taataatatt aatcttttat atggttaata
      661 attatagaag tgtcgttgga aaatctgata ctaaaaatag aaatattaaa aataacacta
      721 ttaaagacat atctactaaa aaagggaaaa attatagggg tttttccgta ttagaaattg
      781 taaaaatact attacttttt gctacaggat tgataatgat gataataggt tcacaaatcc
      841 ttatagagag tggagtaata atagctagtt ttttaaatat acctcagggt atagtaagtt
      901 taactataat agcacttgga acttctcttc cagagatagt ttcgtcgatt actgctataa
      961 gaaaaaatca tcatgaaata tcagtaggaa atattttggg tgcaaacata ttgaatatag
     1021 tatcagtaat agctgtatca gcaataccaa ataacatacc aatactatct caaaatagac
     1081 agttggatat accattcatg atattattat tattaatagt aataatacca acacttaaat
     1141 ctaataaact tagtagaata caaggtatat tgatgttatt cacatatttt ctgtacatat
     1201 caattttgta tttcatgtat attatttaac tatactgttt aaaatattaa ttgcagaata
     1261 tcctaaaaaa gtatataatg gataggttaa gattaattga taacggagag gaataatata
     1321 tgaatataac aaagtttgaa gatttaccaa taagtgaggg aataaaaaaa gctatagctg
     1381 aaatggggtt tgaagaacca tctcctatac aagcacagtc tatacctgcg attttgtcag
     1441 gtaaagacgt tataggacaa gctcaaactg gtacgggaaa aacagctgct tttagtatcc
     1501 ctatactaga aacaatagac ccaaataata gaagtttaca agctgtagtg ctttgtccaa
     1561 caagagaact ggcaatacaa gtatcaacag aaataagaaa gttagctaag tattcgcacg
     1621 gaataaagac attacctata tatggtggtc agccaataga tagacaaata aaatctctaa
     1681 agagtggtgt gcaagttgta ataggtacac caggacgtac aatagaccat atcaatcgta
     1741 aaacattaaa gatggataat gtaaaaatga ttatattaga tgaagcagat gaaatgctag
     1801 atatgggatt tagagaagat atagaaatga ttttaagtaa gattccagaa gaaagacaaa
     1861 caactttctt ttcagctact atgccaagag gaattttgga attaactaag agatatcaaa
     1921 aagacccaga gcatataaaa gttgttagaa aagaacttac agtttcaaat acaaagcaat
     1981 actatataga aactaggtca tcaaataaat tagaagtatt atgtagatta gtagatgtat
     2041 atgaccctaa actatctgtt gttttctgta atactaagag aaaagcagat gaattggtag
     2101 gagatttaca agcaagagga tattttgcag atgcattaca tggtgactta aaacaaactc
     2161 aaagagatat agtaatggat aagtttagaa atggtacaat agatatactt gttgctactg
     2221 atgttgcagc tagaggtata gatgttgatg atgttgaatg tgtattcaac tatgacttac
     2281 cacaagatga agagtattac gttcatagaa ttggtagaac tggtcgtgct ggtagagaag
     2341 gtatgtcatt tacttttgta tttggaaaag aaatgagaaa gatgaaagat atagaaagat
     2401 atactaaatc taaactaata aagcataata taccaacaat aactgatgtt gaagagaaaa
     2461 aagtaggtac tttctttgca caagttaaac aaactataga agaaggtcac ttaactaaac
     2521 aattacaatg gttagaaggt ttctgtaatg atgaagatta tgcaatggtt gatatagctg
     2581 ctgcacttgt aaaactatct ctaggtgaag aaatgaaaga agaaataata gaagaaaaac
     2641 ctagaagaga gcgtggagat agaaaaggtg gtactggagc taaagatggt atgattagat
     2701 tatttatcaa tattggtaga aatcaaagag tacaagcaaa agatatagtt ggtgccatag
     2761 ctggagaagt tggaatacca ggtaaagtag taggtactat agatatatat gataagtata
     2821 cttttgttga aatacctaaa aaagatgcaa aaacagtaat tgaaaagatg aaagatatta
     2881 agataaaagg taataagatt aatatagaaa aagcaaataa gaagaaaaaa taacgtatag
     2941 tatacaaaac tataattaga aaatatataa aataatatat tggatattta aaaagcctga
     3001 tgtgttttca aaacagatag tagataatct gtaagagtta acagcataag gcttttcttg
     3061 ttttatatga tgttataatt ttccagatta ttgtgaaaaa tgtatattag ttcctttttt
     3121 tcaaatctga tttgaaaaag agaatattaa ttatctatat agtttagatt ataataaaaa
     3181 atgtaagata acaaagggga gaaaaagatg tcaatgactt atatggaccc tagggttttt
     3241 ttgattttag aaatggtgtt agaagaagac acaaatactc tagaacaatt ttcaaatgtt
     3301 ttgggtgtta gtactaggac tatcagaaac tatataaaac agattaatcg agaattaaaa
     3361 ggtggtattg ctaaaatagt taaaaatgaa aaaggtacat atagtataag aattgaggat
     3421 gaaagtaaat taattgagat tgtaaatttt aatagaggaa agctatcaaa ttttataaat
     3481 ttaaattcac ctgatgaaag aataaattat gttcttgata ttttaactat gaccaacaat
     3541 ccaattacca tagatgattt ggctgaagag atatgtatag gaagaacaac attaataaag
     3601 gatttaaaaa aagcagacag aattttagct gaatatgatt tagaattaaa aaggaagcca
     3661 aatataggta tgattttagt tggtgaagaa atggatataa gattattaat ccttgataga
     3721 ttgtatgaaa attatattga tgtcttggaa aacgttaatc agataaattt agttaaagat
     3781 tatgatattg aatgtcttag agatgaatta aaaaagttat ttaaaaaaga agaactctac
     3841 ataacagaac aggtcttgag agatgttgaa agatatataa tcatgtctgt cttaagaaat
     3901 ctaaatgggt acaaatttga aaagatagat aagagatttg aagtgataag atgttcagat
     3961 gaatatactt taggattaaa gctcaaagct ttattggaaa agatatttaa tatagtttta
     4021 aatgaaaaag aaaccatatt tttgacaatg ccattaatag gtagaaaagc tcctagtaca
     4081 aaattagcac ttagcagtat aaagataaat gatagcgtaa aggaagttgt agatgaagta
     4141 actagttttt tattagagac ctcagggatt gattttaaag aagataaaaa gcttatagaa
     4201 aatttggaat atcacttgta ttttgcactt aatagaatga gattcaacat aagggtaaaa
     4261 aatcctcttt tacaagaaat aaaggaaaga tatacatttc catatagtgt tgcaaagata
     4321 gctgcaatga taattgagga aaagtttgac ttgaaaatat gtgaccacga aataggatat
     4381 atagcattac attttggaag ttattttgaa aaaaattcaa gaaaagttct aaatgtaaat
     4441 agggtagctg ttgtttgtgg aacaggtctt gggactgcac aacttattag aataaaggta
     4501 ggaaaaatac ttggagaaag tgtagaagta aatacttttt ctgatataga agctaaagct
     4561 aatttagatt tattaaagta ttatgattta gtatttacaa cagtagattt caaaacagac
     4621 ttagatgttc caatttttag agtaaatgcg ttatttaatg aagaaagtat aaagaaagag
     4681 atagaaaatg caatactttt gaaaaatagt ataaatctta agtctataaa aaagattgaa
     4741 aaaaatatcc cttttatagg taaattaatt gaagaggata agttctttat attgaataag
     4801 agtacgttta tggaaaattt agaagatatg ttagaaaaat tagttcataa aggaattatt
     4861 gatatagagt tcaaaaaaaa ggtattaaaa agagaaaaga aatcttcaac agcattagat
     4921 aattatatag cattgcctca ctcagttaat actaatgggg aaaatcttta tctggcattt
     4981 ggaatattag aaaaaccagt gatttgggat acaaaggaaa ttagaataat aatattgatg
     5041 ataattccag ataagaatgt agatagtaca gacctgataa taaaaagtta tgaagaagta
     5101 cttgaagtag ggagagataa aaagatggta gaagaacttg ctaaggtaaa atcatttgat
     5161 gaattttata aaataattac caagaggagg taataatatg gacccagtaa tttttattat
     5221 agcatcactt attacagtgt atttattaaa tatttttctt ggatacttac agcttaaaga
     5281 ttttaataaa aattatattg aactaaaaag aaaaggaaga gtggcaattg gcagaaaaaa
     5341 aggaagaatt tcatcgggta caattgtgtt gatacttata aatgatgatg gtgtgattgt
     5401 cgagactaga aaaatgcaag gtgttacagt attggcaaga gtaaagtcat ttgaaggttt
     5461 ggtaggaaag agcttagagt atatagaaaa atcagattta actgtttaca ataaactact
     5521 taaaaaagca atactagatg cagtcaaaca gtacataaca tttgagaaaa ataaatgtga
     5581 agatacaaat aatctgagta ctgatgaaga gtgtaggatg gaagtagttt aaaaaaatat
     5641 atttataagg aggagttaat atgacgcaat tttttcaagt attagcaagt ggtgcagata
     5701 gttttatgaa tttatttagg gcaggtgggc aacaatttgt aggttttgta acagatatag
     5761 taccactttt agtagcatta ttagttacaa tgaatgcaat tatcaacttt attggtacag
     5821 atagagtcga gagacttgca aaaaaatgtt ctgcaaatat atttactaga tatttgatat
     5881 taccagtcct tggtacattt gtatttgcaa acccaatgac actatcatta ggaagattct
     5941 tgccagaaaa gtttaaacct agttactttg cagcagcaag cttttcatgc catacaatga
     6001 atggattatt cccacatatc aatccaggtg aactatttat atatctagga attgcaaatg
     6061 gtataacaac tttgggattc tcaacagcag atttagcagt tagatatttg ttagtgggta
     6121 tagttgcaaa ctttattaaa ggtgtagtta ctgactatac aacaaaattt gtagagaaac
     6181 aacaaggaat agcattaaat tcagatataa agattggata attactttaa ggtataaata
     6241 aatttttcaa ataaaaagat agtgtataga ggaggataaa ttatatggca tttaacaaag
     6301 ttaaaatagt aaaaggttct ggaggatggg gtggaccatt gataatagaa ccaacagaaa
     6361 agaagaataa ggtagtatat gtgactgggg gagcaaagcc agaaactgca gtaaaaatag
     6421 ctgaacttac aggatgtgag ttaatagatg gattttcaca gggtgttaaa gatgatgaaa
     6481 tcgcatgtgt aataataaat tgtggtggaa cattgagatg tggtatatat cctcaaaaga
     6541 aaataccgac tataaatata atgaaaacag gaaaaagtgg accattagca atgtttataa
     6601 aagaagatat atatgtttca ggggtaaaac caacaaatgt agagcagtta taattgaggt
     6661 agtaggaggg aagaaatatg gcttatgata ctaaaaagaa gataagtgag cagagtcaag
     6721 gaattgttgc aaaaataggt atagcactag gaaatgtgac aagtactctt tttcaagctg
     6781 gtagggatac tatagataca ttaatacata ctatactgcc ttttatggct ttcgttgcag
     6841 tcctaatagg tataataaat caatcgggta taggaaattg ggtagccaat ttacttactc
     6901 cattaatcgg aaatgttgga ggtttagttg tcttatcaat aatatgttca tttccgttgt
     6961 tgtctccgtt tcttggacca ggagctgtta tagctcagat agtgggggta ttggttggta
     7021 cagaaatagg aaaaggaaat attccaccaa gtttagcatt accagcattg tttgcaataa
     7081 actcacaagc agcttgtgac tttataccag taggtcttgg tcttgcagaa gctgaacctg
     7141 atacagttca agtaggagtt ccgtcagttt tatattctag atttttaaca ggtgcaccaa
     7201 ctgttctatt agcatggtta gcaagctttg ggttatataa atagaagaat actttataaa
     7261 taattagaga gaggttcagt tgatatggaa ttaatataca gtactcaaat aaataaaata
     7321 ggagataatg caaatgaatt tttacaacat aatatgtttg taactttcaa agataatgct
     7381 cctcaagagt tagaaaatta ctgttatata cattctgaaa ataatttagt aaaagaaata
     7441 cttacggatg atgtgctatc aataaataat gttgattaca aaattacttc tgttggtgag
     7501 ttagtaaacc aaaatctttc agaattaggt catataacat ttaagtttac aggtgaaaaa
     7561 gatggagcaa ttggaggaac cttatatctt gaagaaaaag aaatagcacc tattggcgta
     7621 ggtactatac taaaaataat tagaaaataa aatttaaaac gaatctaatc atattataag
     7681 taaattatag ataaaagtca atttaactaa tcaagttaac ttttaatgtg ataaaaaagg
     7741 ggaaatgaaa atgagaaaag tggctttagt cgttggagga ggaagaagtt taggtgaata
     7801 tttatctaaa catttagcta aagtaggtta tgatgttgct attgcagatt taaatgaaga
     7861 caacgcaaaa aaagtagctg aagaaataaa aactgaattt ggttgtaaat ctgtaggata
     7921 tagttgtaat gccatagatg aaagacaggt tataagtact gtcaaagcta ttgaaaatga
     7981 ttttgataga atagatttat tagtgtacaa tgcaggagtg gctgtaagta aaaaaataac
     8041 tgattttgga tacaaagatt ttaaatttat tgtagacgta aatttaaatg ggtactttct
     8101 atttgcaaga gaagtatcta aagtcatgat agcaaaaggt attgagggta gaataataca
     8161 gataaattca aaatcaggta gagtgggaag caagcataat tcaggatatt cagcaggtaa
     8221 atttgctgga gtaggactta ctcaaagttt agcgctagat ttggcagaac ataagataac
     8281 tgtaaatgca ctgatgctcg gtaatttact tgattctgat atgtttgaaa gtttaattcc
     8341 tcaatatgca gtaaaattgg gaatacctga atcagaagtc aagcaagttt atatagataa
     8401 agtaccttta aaaagagggt gtagatttat agatgttgca aatatgctta cattttatgc
     8461 ttcaaaggaa gctgaatact gcactggtca atctataaat attactggtg gacaagttat
     8521 gtaaggtttt tatggagtgt ctcaagatga actctttagt tcatgaggca cttttttgta
     8581 taaaattaaa tatattttca gcatttaaac aatgaaaaag aggtttatct ttattttgag
     8641 atagcctcat ttttgtaatt aaaaaacata gtgtttaagt aaacatataa gatt
//
LOCUS       DACXMZ010000170         8649 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.171, whole genome shotgun sequence.
ACCESSION   DACXMZ010000170 DACXMZ010000000
VERSION     DACXMZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.171"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(20..535)
                     /locus_tag="JD016_14640"
     CDS             complement(20..535)
                     /locus_tag="JD016_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HAU5000868.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(898..1968)
                     /gene="leuB"
                     /locus_tag="JD016_14645"
     CDS             complement(898..1968)
                     /gene="leuB"
                     /locus_tag="JD016_14645"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAU5000869.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGSVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(1975..2466)
                     /gene="leuD"
                     /locus_tag="JD016_14650"
     CDS             complement(1975..2466)
                     /gene="leuD"
                     /locus_tag="JD016_14650"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAU5000870.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2481..3758)
                     /gene="leuC"
                     /locus_tag="JD016_14655"
     CDS             complement(2481..3758)
                     /gene="leuC"
                     /locus_tag="JD016_14655"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAU5000871.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAKLKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3715..5457)
                     /gene="leuA"
                     /locus_tag="JD016_14660"
     CDS             complement(3715..5457)
                     /gene="leuA"
                     /locus_tag="JD016_14660"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAU5000872.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISKTC"
     misc_binding    complement(5581..5827)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5975..6421)
                     /locus_tag="JD016_14665"
     CDS             complement(5975..6421)
                     /locus_tag="JD016_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000873.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6442..7161)
                     /locus_tag="JD016_14670"
     CDS             complement(6442..7161)
                     /locus_tag="JD016_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAU5000874.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPRKVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(7279..8553)
                     /locus_tag="JD016_14675"
     CDS             complement(7279..8553)
                     /locus_tag="JD016_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAU5000875.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
BASE COUNT         2620 a         1719 c          880 g         3430 t
ORIGIN      
        1 atgtaaaatt tttatttaat caaagttcaa tatattccac tttttctttc gattcttcaa
       61 aaaaccattc ttcattagaa aaatcacctt ctgcaatttg tccaataggc attttcatat
      121 actttccagt tgctgtcact gctatatctc catttggtag aattatttct cctgtacctt
      181 caaataattt tctgttttct ttagttattc ttccaacaac ttttatagta gcatctgttg
      241 gtactggttt cttatatttc aactctaaag atactgttac tccccatatt tggtcatcat
      301 ttatacttac agctcttcca atagtctcat ccaatattgc agcagacata cctccatgaa
      361 ctctcccagg ataactttga tgccaatcct ttgtattaca aattgataca agctctccat
      421 tttcaagttc ataaaaatct gctttaagtc ctagctcatt ttgtgttcca caaactaaac
      481 aagatttact actattttgc ttcttcaaaa ctttatactt tgccaaaaaa tccatctcct
      541 aaccgacatt agtagtcgct taatattaaa taattctaaa atatattata taatattaat
      601 aaaatccctg caaacttcta ataataaaaa taggctactt caatatgata agtttaattt
      661 aaagtattat tttccaaaaa ataactttat caaaatagaa actcaaagta gatataaacc
      721 tctttttcgt tattaaaata gtaaaaatat atttgactta atataaaaaa gagtgtctca
      781 ttaactaata aagtttattt tgagacaccc ctcataaaac aaattatact acaatagatt
      841 tttactaatt ctacaagtta tatattcaaa ttatattatt caaattaaac tataattcta
      901 aatttcattt actattaatt cgcccatttc ccttgttcct acaacatttc ccacaccatt
      961 atagatatca atagttctat aacctttatt gagtacactt tgtacagcat cttcaataca
     1021 cttagcttct tcttctaagt taaaactatg tcttaacatc atgcttacag atagaatagt
     1081 cgcaattgga tttacaatat cttttccagc tatatcaggt gcacttccat gtattggttc
     1141 atacatacca aaactatctt ctctaagtga tgctgacggt aacataccta tagaacccgt
     1201 aatcatagat gcttcatcag atattatatc tccaaatata ttattagtca ctattacatc
     1261 aaattggcta gggtctttta caacttgcat agcagcatta tcaacataca tatgtgacag
     1321 ttctacatct tcaaactctt tagctaagtc ttccattata cttctccaaa gtcttgaact
     1381 ctcaagcaca tttgccttat ctacacttat aagtttttta tttctatttc tggctatttc
     1441 aaaagctctc ttaccaattc ttcttatttc attttcgtta tatatttcta catcataagc
     1501 attttcgata ccatcaatga tttttcttcc acgttctcca aaataaatgc cccctgttaa
     1561 ttcccttaca acaacaaaat caatgccttt ctcaactata tccgttctaa gtggtgaagc
     1621 ttcttttata ctttcataca ttgtagctgg tcttaaattt gcaaataaac ccaatgactc
     1681 tcttaatttt aaaagaccag actctggtct tttatctccc ttacatttat cccactttgg
     1741 tcctccaaca gaaccaagca atacagcttc gtttttccga caagtttcaa cagttttatt
     1801 aggtagaggt tctccagttt catcaatcgc gcatcctcca gctaatacat attcacaatc
     1861 gaatctatga ttaaatttac aacaaatttt atttagaacc tttatacctt catctataat
     1921 ttccggtcca acaccatcac cttttatgac tgctatatta caattcatac tccactaccc
     1981 cctatttgct tttattgagt taattaagcc ttcatttaat ataatatttt gcataaactc
     2041 aggaaatggt tctgctttat attcttttcc ttttgttata ttttttatca cacctgtaga
     2101 aaaatctact tctatattat ccccttcatc tatgttattg gcagcttctt cacattcaag
     2161 tataggaagc cctatattta tagagtttct aaaaaatatt ctagcaaaag tagaagcaat
     2221 cacacaggat actccacttt cttttattac aattggtgca tgttctcttg atgaaccaca
     2281 accaaagttt ttagttgcaa ctataatatc tccttttttt accttagaaa taaatttatc
     2341 atcaatatcc tccatacaat gcgttgctag ttctttataa tcagctatat ttaaatatct
     2401 agctggtatt attacatctg tatctatatt gtcaccgaat ttaaaaacac ttccattggc
     2461 aatcataaat tttacctccc ttaaattatt tctgatggtt ttgatatttt tccagttata
     2521 gctgatgctg ctgctacagc tgggcttgca agatacacct ctgaatcaac atgccccatt
     2581 cttcctacga aattcctatt tgttgttgaa atggattttt ctcctgctgc aagtataccc
     2641 atatgtcccc ctagacatgg accacaagtt ggtgtagaaa ctgctgctcc tgcctctata
     2701 aaagtggtaa tatacccttt ttctatagct tcaagatata ctttttgagt tccaggaaat
     2761 atcataactc taactccatc tgctactttt ttgcctttca ttatctcagc tactacttca
     2821 aggtctgata ttcttccatt tgtacaagag cctattacaa cttggtctat ctttatatcc
     2881 ttatctatct catctactgt tctagtattt tcaggaagat gaggaaaggc tactgtttct
     2941 tttagtttgg ctaaatctat ttcgtatact tcatcataga ctgcatcttc atctgcttca
     3001 tatatattta catcttttgt catagttgaa cacttatgtg aatttatata ttccatagtc
     3061 ttatcgtcta ctggaaaaat tccatttttt gctcctgctt ctattgccat attacaaata
     3121 gtaaatctat catccataga taagtattct actccatctc cacaaaattc cattgacttg
     3181 taaagtgctc catcaacacc tatctcccct attatatgaa gtattatatc tttaccactt
     3241 atccatttat ttggtttatt tttcaaaaca aattttattg ctccaggaac cttaaaccaa
     3301 cattttcctg tagccatacc tgctcccata tcagttgaac ctacaccagt agaaaatgct
     3361 cctaatgctc catatgtgca agtatgagag tcagcaccta taattacatc tccagcagtt
     3421 actatacctt tttcaggaag aagaacatgc tctattccca tatctcctac atcaaaaaaa
     3481 tttacaactc cttttgattt tgcgaaactt cttgtaaatt tacattgttc tgcactcttt
     3541 atatctttat taggtgtaaa gtggtctaaa accatcacta ctttagtctt atcataaaca
     3601 tcttcaattc ctaatttttc aaattctcta atggctactg gtgatgttat atcattacct
     3661 aaaactacat ctaaatctgc ttcgattaat tgccctttct taacttcatt taatttagca
     3721 tgttttgcta atattttctg cgtcatagtc atccccattt tttatttcct cctcaatata
     3781 acttttattt attgcattca taagtgcatt aatagctgct gtatttatgt tttccgatat
     3841 acttacacca aaatagactt cattattgtg ctctattcct acataagcta cccctcttga
     3901 atgtgaccct tcctctaatg catgttccca gtaatatcta aatttataat ctttcatgcc
     3961 acattgcttt agtgcattat taaatgaatc tacaggtcca tttcctatac caacaatcct
     4021 ttgttcattt cccatataac ttattcttgc agtcatctca atcatgttag tatcattaga
     4081 atcatcattt tcatagttta attcatccat tgattttatt ttatacttct taactttata
     4141 agggctctca acttcaatat attctttctt aaacaaatta aatattgctt cactactaag
     4201 ttcttcccca attctatcag attccatttt gactacattt ccaaaatcag aatgcatatt
     4261 cttaggtatc ataaatccat aatctgtttc cattatataa acagctcctc cttttccaga
     4321 ttggctattt atccttataa tttctttgta ttctcttcca atatcatgag ggtctattgg
     4381 taaatatgga acttcccagt aatcattatc tctgtttttc attgctatca tgccttttct
     4441 aatagcatct tgatgtgaac ctgaaaaagc acaatgtaca agattacctg catatggatg
     4501 tctatcatgt accataagtt ttgtacagtc ttcatatata cctattatct tatctatatt
     4561 gctaaaatct agctctgggt ctattccttg tgtataaaga ttaagtccca catttactat
     4621 atccatattc ccagttcttt caccatttcc aaatagtgtt ccttcaagtc tatcggcacc
     4681 agccaataat ccaagttcac ttgcagccgt acaagttcct ctatcattgt gagtatgcaa
     4741 acttagtata atagaatccc tacaacttat atttttacaa aaccattcta tttggtctgc
     4801 atatacgttt ggtgttgcca tttcaactgt tgatggaaga tttattatta cttttttttg
     4861 aggagttggt ttccaaacat ctatcactgc ctcacatatt tcaagtgcat aatccatctc
     4921 tgttcctgta aaactctctg gtgaatattc aaaagtaaat tctgtttctg gatatttttt
     4981 tacttcttca ttaaataatt ttgctccttc aactgctata tctattattt cttgtttact
     5041 catattaaaa accacatctc tttgaagaac agatgtagag ttatataaat gtataattgc
     5101 ttttttacat ccccttattg attcaaatgt tttttctata agatgagccc tagattgtgt
     5161 aagtacttgt atagttacat catctggaat catattctca tctattaatt ttcttaaaaa
     5221 tgtatactct gtatttgatg ctgatggaaa tcctacctct atttctttaa atcctgtatc
     5281 aataagcatt ttgaacattc tgactttttc atttacactc ataggtgttg gcagtgactg
     5341 atttccgtct cttaaatcga cactacacca aattggagcc ttaaatattt cattatctgg
     5401 ccatttacga tttactattt gcattttatc atatttttta tactttccac acttcatgtt
     5461 atttccccct ttttctgttt tttgtattca gtatacctca tatcgacgca atcctgcaca
     5521 attcataagg ataaatgtca attttatcat tagaataaaa aaaacctcat ctctaataaa
     5581 attagagacg aggttttgct cgcggtacca ctctatttag ctttctaaaa agctccctta
     5641 gtaatagcca atacaataat tataatgact atatttccta taacgtggaa ttactccgat
     5701 tattctaaga tttaaatctt agaaaacagc tcctagacaa gttcagaact atcaactact
     5761 aaatcacacc attctttagc tctctgaaag tttcattgtt cttactactt ctattcatca
     5821 cttttttaat catttaaatt ttctaaatat tttgatttaa tagttattat atacacatta
     5881 aaatgtattg tcaacacaaa aaataattat ctcaattttt tatttttgat aagcaattct
     5941 ataaaacatg ctaactattg aaaacagtat gatattatgc cttaaacttt ttcatctttt
     6001 catctatgat attcaataat tttttttcat cttccaaact caatgccctt aatctaattt
     6061 tataattaga ttcatcagtg tatatattta atatattttt tactacttct attttttcta
     6121 ttatcttata attaaacttt tgactttgtt tagattttat actgaatata cattgattat
     6181 ttattaatat tgaatgatta tcgagagtaa tatatacttt tccctcatat ttatttgaaa
     6241 aatcacgacc ttgtttaaat agaaaaactg ctgttattat acataaaata tataatattg
     6301 tcgttgaata tataaaaatt gtagctaaaa ttataagtgc taaaaccata tatatagtca
     6361 ttcctttttt aaaataacca ttgtcataat aattcatata gataaactta tttttactca
     6421 aattttttca ctcccaagat tctaaaaatt tatatcaaaa aattctataa tttcatcttc
     6481 atgataattt atgactaatt catctggaaa atctactgct tctaaaactt tttctgcatt
     6541 ttcaaattct cctatagaat aacatatatg agagtcagtc ccaagaatta ctcttgcacc
     6601 atatgtttta cacaatgtca acatatgact tacattttcc caagtacccg ttctatgaga
     6661 gtttgaactt aatgatgaat tatttatttc taaaaggata tttttatctt tagctttttt
     6721 tactataggt tcataatcta aaggatacct actgtcatct ggatgtccaa ttatttttac
     6781 cttaggttta tccataactt ttaaaatagc atttgtattt tcttcttttg taccagaagc
     6841 tatacaaggt ctatgcaaac ttgcgattat ataatccaaa tgttgcaaca tatcttcttg
     6901 aacatctaaa ttaccattat aatccattat atttccttct attcccctaa gaatctttac
     6961 gccttgcact ttacgtggaa gcaaatgcaa attataaaaa tagaatggat gtggtcctcc
     7021 tggcatatta ggtccgtggt ctgatattcc taagtatttt atattcttct tactcgcctc
     7081 ttcgacattt tccttgaaag tactatgtgc atgtccactt attagagtgt gacaatgtac
     7141 atccataata gactttttca ttaaatttcc tcctaatatt ttacataaat tcgtgaaaat
     7201 agctaaaggg ctatttcaaa actaaagctg tcataataga cagtgtaacg ttttaaaaca
     7261 gccccatttt caattatttt acacttctaa atctttaaat ttatctttaa ataattctcc
     7321 aatacttcct aaagattcct ctacttctag gtattctgtt atatcctctt ctggctctct
     7381 agttgcttct tttattgaaa ccaacattct tttagtcttt ggtttaaaat caagaatctt
     7441 aactttaacc tcatctccaa tattaacaac attagtaact ttagccactc tagtatcact
     7501 caattcagat attggtaaat acgcatctac accttcttta atttgaacaa aagccccttt
     7561 ttctattatt ctaagaactt tagcattgac aacatcatca atttgtactt cttttgctat
     7621 taaatcccaa gggtcattat ttatgtcttt taatgccaag gcaatttttt tagtctcttt
     7681 atttacatcc aacacataaa cttctacttc ttgtccctct gaaacaacat cttcaacact
     7741 ttcaactctt gtccaagcta aattatttat atgtgctaat ccttcaatcc caccaatatc
     7801 cacaaatgca ccataaggca ttatctttgt caccttacca tctctttttt gtccaacttc
     7861 aagagaatta aataagtctt ctttagctgc tttgatacga gctttttctt cttctctttc
     7921 ttgcttaatt cttgctcttt cagcttcttt ttctgcctta atttgagctc ttctttcttc
     7981 tctctcttta tttatacgct cttgtaatac atctctatga gatgctacaa gtctattttt
     8041 tctaacatcc aattctttta tataaacttc taagttaaga ccaacaaatt tagatgtatc
     8101 tgtaataaat tttgtatcaa gttgagaaat tggtataaaa aatgtatatg tattgtaatt
     8161 agcaaataca cctttatcta tattcttttc aacatttaca gttattattc tatgttcatc
     8221 ataagccttt tgtaactcgg caaaatcatt tcttttgtca agttgtaatt tagataaagt
     8281 tattgtacca tctttttgag atacttttgt tattacagca gttatttcat cacctatgtg
     8341 gtatatatct tctatatatt gatttttttc tatgttcaat tcacttatag gtattattcc
     8401 atcaaaccca tagtctaatc ctaattgtac ttcatcattt ctcactgcag ttatttttcc
     8461 agttgtcaat tctccaactt tcactttgct gaatacttgt tcttgctggt caagtaattc
     8521 ttgcattgtt aaatcatttt ccattataaa catcctttcc tcttattgat tatcatatct
     8581 gtaaatatta taccattaat attgcaaata cttacatttt tcttactata atttttaaaa
     8641 taaattcaa
//
LOCUS       DACXMZ010000171         8636 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.172, whole genome shotgun sequence.
ACCESSION   DACXMZ010000171 DACXMZ010000000
VERSION     DACXMZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.172"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            53..820
                     /locus_tag="JD016_14680"
     CDS             53..820
                     /locus_tag="JD016_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAU5000876.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            1103..1300
                     /locus_tag="JD016_14685"
     CDS             1103..1300
                     /locus_tag="JD016_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAU5000877.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            1329..2405
                     /gene="vorB"
                     /locus_tag="JD016_14690"
     CDS             1329..2405
                     /gene="vorB"
                     /locus_tag="JD016_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HAU5000878.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            2408..3127
                     /locus_tag="JD016_14695"
     CDS             2408..3127
                     /locus_tag="JD016_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HAU5000879.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            3140..3679
                     /locus_tag="JD016_14700"
     CDS             3140..3679
                     /locus_tag="JD016_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAU5000880.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            3836..4894
                     /gene="buk"
                     /locus_tag="JD016_14705"
     CDS             3836..4894
                     /gene="buk"
                     /locus_tag="JD016_14705"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HAU5000881.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLLGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            4912..5805
                     /locus_tag="JD016_14710"
     CDS             4912..5805
                     /locus_tag="JD016_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HAU5000882.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            5835..7043
                     /locus_tag="JD016_14715"
     CDS             5835..7043
                     /locus_tag="JD016_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAU5000883.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            7067..8326
                     /locus_tag="JD016_14720"
     CDS             7067..8326
                     /locus_tag="JD016_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAU5000884.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT         3346 a          964 c         1732 g         2594 t
ORIGIN      
        1 tttagtagag tgtgggagta gcacttaata tttgcaggag gagtttttgt atatgggaga
       61 aataataaat gcattagata gagccctaga cataattttg ctgttatatc atgaaaagag
      121 agaaatggga ataactgaaa taagcaaggc tatgggagta tataaaagta ctgtacatag
      181 aactttggtt actcttgaaa acaaaggttt tgttatacaa aatgcagaga atggtaagta
      241 ttggctagga ataaatttat atgcaatagg tatggttgtg ggagaaaaaa tgtcattgac
      301 tgaaatagta aaaccatata cgaaaaaatt aaatcaagaa tttaacgaag tagttaatgt
      361 atctatatta gaagaaagag ctcaagattc tccaagaagt atcataatac ataaagagta
      421 tggttcaaat caattacttt cagtaaatcc atccgttggt tcaagtagtg aatgttattg
      481 ttcagcagtt ggtaaatgtc ttatggcatt taatgattct atagattttg aaaaatatag
      541 aaaaacacca atacacaagt atacagaaca tacaattgat aattgggatg atatgatgct
      601 ttttttggca aaaataaaag aacaaggata tgctatagat gatgaagaac tagaacatgg
      661 actaacttgt attggagcac ctatcttaga taaaaataat aaagctattg cagccataag
      721 tttgtcaggt ccaactataa gaatgagaga aggggacttt gagtataaga ttaaaagagt
      781 tatagaaact gcaaaaagta tatctgaact ttttagatga tttttaatct cgataataaa
      841 aataggctat atttaaattg ttatttgtaa atatcagttt aaatgtagcc tatttttctt
      901 taatctaaaa ttaaatattc ttaaaattta aaatgttatt gaaatatata agcgaatgtg
      961 ttatctttta attaagcaat aaaaatagaa caaggttcta tactatagaa caaaattgat
     1021 gcatatatag tatttataca aaatataaat attatatatt taaaattaag taaaaatatt
     1081 acataaaggg gagatttgag aaatggaaaa agtcaagttg agtgttgaaa gatgtaaggg
     1141 atgctatctt tgtattgaag cttgtaaaaa agatgcaata agtctttctg actacacaaa
     1201 taaaaaaggc tttgtaacta ttaaagtcga cgaagaaaaa tgtattcaat gtggcgcttg
     1261 ttatacaatg tgccctgatt tagtatttga gatattataa attattttta ttagagggga
     1321 gaaaaggtat gcaaagttta ttaattaaag gaaatgaagc tttagcagaa ggtgctatac
     1381 gtggtggctg cagattttat gcaggttatc caataacacc acaatcagaa atattagaaa
     1441 tgatggcatg gagacaaaaa gaagttggag gagtatttat ccaaggtgaa tcagaaatag
     1501 ccagtgtaaa tatggttatg ggagcaagtg cacttggagc aagagctatg acttcttcat
     1561 ctggtccagg attttcatta aaacaagaag gtattgcata ttgggtatca gctggtttgc
     1621 cagcagttgt agcatgtgtg caaagatatg gtataggtga tggatttata ggagctggtc
     1681 aagataatta ttggcaagca gtaaaaggcg gtggaaatgg agattatcat ttaatcgtgt
     1741 tagcacctaa tagtgttcaa gaaaatatgg atatggcata tgaatctttt gaattggctg
     1801 aaaaatatat gcatccagta cttatacttt ctgatgctac aataggtcaa atggtagaac
     1861 cttgtatagc accaccaatg aaagaatacg atatggataa agctccatgg gctgcaaaag
     1921 gaacacctgt aggagaaaaa aataaagtaa taactgatat aacatatttt gaagaccaag
     1981 atacatggca aaaagggtat atagaaaaaa tgagaaaagt tcaagaagat gaacaaagat
     2041 gggaagagat agaaatagaa gatgctgaag tggtatttgt ggcatacggt atttcatcaa
     2101 gaatagcaga aggtgcagtt agagatgcta gagcaaaagg aatgaaatta ggtctaataa
     2161 gaccaaaaac attgtggcct tacccaagaa aagcatttaa gaaattaagt gattcattga
     2221 agggacttgt aacagttgaa atgaacatga tggcacagat gaaggaagat gtaattatag
     2281 catgtgataa tagatttcca gtatattcag tagcaacagc tcaatatatg gcagaagtaa
     2341 atcaattaat agattttgct caacagatta tagatggaaa agctaagcaa gaggaggtat
     2401 tttaattatg gctaaaaaag caccagcaat aatgatgtgt gaaaatatgt tctgtggagg
     2461 ctgtggacac tctatagtcg atagaattat tggagaagta ttaacagaaa tggatatatg
     2521 tcaagataca attatatgtt cagatatagg ttgtaatcat atgtttcaat ttagtatgaa
     2581 tgtagatgtt gtcgttccac ctcatggtaa aatgggagca gcaatggcag ctatgaaaag
     2641 agttagacca gataaatata tatttacaat agctggagat ggtggagctt atgcaatagg
     2701 gcttggagaa actatgtcag ctgctactag aaatgataat gtaatatttc ttgttatgaa
     2761 caataatatg tttgcaatga caggagggca aatggctcca actactatag aaggtcaata
     2821 tacagcttct actccagcgg gtagaaatta cgcagatcat ggttcaacat ttgatgttgt
     2881 aaaagttatg ggaaatttag atatagcata tctagcaaga ggaacaataa ctaaccctaa
     2941 agaaataaat acaacaaaaa gatacatcaa aaaagctcta gaaaaacaaa aagaaaagaa
     3001 aggtttttgc ttaatagaag tgcttgtacc atgtccaaca aactggggat taaagccagt
     3061 agatgctatg aagagaattg aaaatgaact tgttccaaag tatccacttg gagaatttat
     3121 tgattaggga ggaaataata tgttgaatga aattatatgt gctggctttg gaggacaggg
     3181 agttcttaca gcaggaaaaa ttataatgta tgttgcatat aaggcagaaa agaaaattac
     3241 atggttccca tcttatggaa atgagatgag gggaggaagt gcaaattgca atgttgttgt
     3301 aagtgatgaa aaaattgcaa atccttgtgt aaaaaatcca gatatactat tgggaatgaa
     3361 cagcgtatca gtagataagt atgaaagttt aatgaaaaaa aatggttata tgtttgtcaa
     3421 tgaatcacta gtaagtaagg atagaaagta cagagaagac attaatgtta taaaagtacc
     3481 tgttactgaa atagcacaaa atttaggcaa tgaaaaagct gctaacattt gcatgttagg
     3541 tgctatggta aagagtttgg atttatttac aaaagaagaa tttgaaagtg gtatgtgtga
     3601 atactttgaa aatcaaggca aaggtaaatt caattctaaa aatgtagaag catttgaagc
     3661 tggatacgac tacgaatgaa tattttaaat atttaaaata attattgaaa tacttatttt
     3721 tatatgttag tatttaatta ataaaatata gaacgtcgtt ctggacaatg ggacgaagtc
     3781 tttaggttgg aatacaagaa cgacgttctg tatagtagaa ctagggggtg ttaaattgaa
     3841 agttttagta ataaatcctg gtggtacatc cacaaaaatt tctgttttcc aagatgaaaa
     3901 tgaaattttg aaaaaaaata taacccatac aagagaagat ttaaaaaatt tttctaaagt
     3961 atttgatgaa tatgattata gaaaacaact gatagtagat attttaagta gtgagaatca
     4021 tagtataaat tcttttgacg cagtagttgg tcgtggtggg cttatgaagg ctataaaagg
     4081 tggtacctat actgtgagtg aagaaatgat tgaggatatg agaaatgaaa taaatgggga
     4141 acatgcatca aatttaggtg cattattagc taaaactata gctgatgaaa taggagtaca
     4201 atcatttgta gtagacccag tttctgtaga tgaatttgat gatgtatcga ggattacagg
     4261 aatatcagac atagaaaaag caagttggtt acatgcatta aaccataaag ctgtttcaag
     4321 aaaaattgca caaaaactag ggggaaaata cgaggattat aactttattg tagctcattt
     4381 aggttcagga atatcgattg tagcacataa acaaggcaaa atgattgatg gaagtggtgg
     4441 taggtctgat ggtccatttt ctccagaaag gagtggtgga ttacttgcat atcctcttat
     4501 agaattgtgt tactccggaa aatatactca tgatgaaatg gtcgcaaaag taagttcgat
     4561 tgggggaatg tacgattatt taggaacaaa ggacatgata gaaattgaaa acagaatcaa
     4621 ggagggtgat gaaaaggcaa aacttataat ggatgcattt atttatcaag tagcaaaaga
     4681 aatttctatg tatggagctt cacttttagg gaatgtagat agagttatct tgacaggagg
     4741 gatatctcac tcagaaatgg tagtcaaagg agtagcagaa aaagtttcat atctagctcc
     4801 aatagagata gtaccaggag agatggaaat ggaggcactt gctctagggg cacttagagt
     4861 acttaatgga gaagaaatag caaaaactta ttaatattta ggagaaagag tatgttaaaa
     4921 gattttaata atttattaga agaggctaaa aagcttgaac ctgttattgt aagtgttgct
     4981 gttgcagaag acaaagaagt attaaaggct gtgaaatcag caacagattt aaacattata
     5041 aagccaattc ttgtaggaga caaggataaa atagaaagta tatcaagaga aattggatta
     5101 aaaggtcatg aaataatagg ttgtcaaaat cccgatgaat cagttaaaaa agctgtagag
     5161 atagttaaaa ataaggaagc taaagttctt atgaagggat tagtaaatac aagtgtctat
     5221 atgagagcta tattaaataa agaaacgggt cttagaacag gaagattatt aagtttgctt
     5281 gcagtatatg aattacctca atatcataaa cttctttatt gtactgatag tggaataaat
     5341 gtatcgccaa acttaaatca aaaaaaagat ataatgacaa atgcacttct tactatgaaa
     5401 agtataggta tggaaaatcc taaaactgcg atacttactg caaatgaaat ggtagaccct
     5461 aagatacaat ctacagtgga tgccaaccta cttgtagaga tggaaaaatc gggagaaata
     5521 ccaaagtgta tagtggaagg tccaatagca tttgatgtag catttgatgc acatgcagca
     5581 gaacataaag gtattaaaag taatattgct ggtgatgttg atttattagt atttccaaat
     5641 atagaagcag ggaatatatt aggaaagtca tggcttagat ttaataaagc taagtgggca
     5701 ggtatagttt taggagcaac aaatccgata attttaggtt cacgttctga cacagctgaa
     5761 ataaaaataa attcaatagc attagcgtgc ctagcatcac aataagaaat tattattaag
     5821 gaggaaaaag tattatgaaa tatgcaaaaa gaatggatat ggtaaaagct tcagctataa
     5881 gagattctca aaagaaaata gctcagaagg tagcaagtgg tggaactgtt atatcttttg
     5941 ctgcagggct tcctgaccca aatttatttc caatagaaga gataagtgaa ataactcaaa
     6001 aagtacttaa tgaaagggga aaatttgccc tagcatatgg acctactaaa ggtgaagatg
     6061 agttgttaga tctattagta aaaagaatga aagaagaaga aaatattgat acaaaagcag
     6121 aaaatattat aataactact gggtcacaac aagggatagc attaagtgct atgattttat
     6181 tagaaaaggg agatgtagta gttacagaaa atccaagtta cttaggtgca ataaatgcat
     6241 ttagacctta tgaatgtgat tttttaggag tagatacaga tgaagaagga atggtaacag
     6301 atgatttaga caaattatta tctgaaaatt ctaacataaa ggtgatatat gtaataccaa
     6361 catttcaaaa ccctacagga aaaacatggt ctttacaaag aagaaaagat tttatggaag
     6421 tcgtgaataa atatgatgtc atagtaatag aggataatcc ttatggagaa ataagattta
     6481 caccagaacc tctacctaca cttaaatctc ttgatactaa agataaagta ttgtaccttg
     6541 ggtcattttc aaaggtatta tgtccaggat ttagagttgc ttggatgtgt ggtaatgtag
     6601 acatcataga taaggcagaa cttttaaaac aaggtataga tttacagtca aatcaatttt
     6661 cgcaagtaca agtaatagaa tttttaaaga aatacaactt aaatgaacat atagaaaaaa
     6721 taagagcaga gtataagaaa agatgtctgc taatgttaga tgctatgaag aaacatttcc
     6781 cagaggaagc taaatataca gaaccagatg gaggaatgtt tatatgggtt gaacttcctg
     6841 aaagtataaa tgtagatgat ttattagata aagccattga tgctggagtt gcttatgttt
     6901 ctggagagtc cttctttgca aataatggtc caaaaaatac tatgagactt aactatacaa
     6961 caatgtcaga agaacaaatc gttaaaggtg ttgagatact tgcagaagtt ataaaaaaag
     7021 aactcaatta tgcttgtgaa taaatataaa aagtgagggt ttaaatatgg gagaaaaaga
     7081 aaacggtata aagtttggaa agttaatgtt tcaacttgtt attatagcaa tatcttggga
     7141 attagtttat attataccat ttatccaata tacattatat gatccaatcc taaaagcatt
     7201 agaatgtaca aacactcaac taggttttct acttacaatt tatgggttag gtaatatatt
     7261 tggagctcct ataggaggct ggttagcaga tagatttgat tatagaaaga taattttagg
     7321 ttctgtattt cttaatggag tagtatcatt tttatttgca tttaatatga actatccatt
     7381 tgcagttgct acttgggtag gttgtgcaat cacatcatta gttatgaact atccttctat
     7441 ggtaaaaata cttagagtta taggaaaaga taatcaaggt aaggtttatg gatttaatga
     7501 agctatggtt ggagtttctg gagtagtaat gggagcaata ttcttatata tttatacatg
     7561 ctttgcaact cctacattgg gtatgagatg ggtaatgata tctttaggaa tattatcaat
     7621 agcaatgtgt ccagtattat ggtttgtaat taaagatgta gatattaaag aagaaagaga
     7681 agaagcaggt gaaaaaatgt ctgctggtga ttttataaca gtactaaaat caccaaatac
     7741 ttggctagta ggaataagta tattctgtgt atattcattc acagttacaa tgtcttattt
     7801 tacaccatat ataacatctg tacttggagg ttctgtagca ttatctggtg cattagcaat
     7861 aataagacag catggtctaa aattatttgg agcaccattt ggaggatatt gtgcagacaa
     7921 attaaaatct cctacaaaag tacttttacc aatatatgta tttggtatag cagtaatagt
     7981 tttattccta gtattacctg catcaactcc aatgactata ttcatagctt taacatttgt
     8041 agtaggtata ttaggttaca tgggaaaagg tatatattat gcagtacaag atgaagtaaa
     8101 agttccagtt aaatattcag caactactat aggtatagct gcagctttag gattttcacc
     8161 agatgtattc caatttgcat taataggaca ctggatagat acttatggca ataaaggtta
     8221 cacatataca tttatattcc aaatagcaat tctagttatt ggaatactat cttgtttata
     8281 tatattaaag gttaaaaaga gaagagcaag taaattagaa gcttaaatat tcaatttggt
     8341 aattaagtat atataaatac aatacttatt ttgatgaatg tgattgtata aataacataa
     8401 aaaatatcaa tagattaagt tttataataa tgtttagaga ataaagtata aaaatcaaaa
     8461 tgttatcaag ttttctaggt tttaacataa agttacccct gaaggtgtaa aattgagtgg
     8521 caattattat ctattttctt acctacataa acctatttaa tttaaattct aagagatttt
     8581 caaaatagat ggtagttgta ctcaatttta aacctcataa taaatacgaa aattta
//
LOCUS       DACXMZ010000172         8609 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.173, whole genome shotgun sequence.
ACCESSION   DACXMZ010000172 DACXMZ010000000
VERSION     DACXMZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.173"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            70..837
                     /locus_tag="JD016_14725"
     CDS             70..837
                     /locus_tag="JD016_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU5000885.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKGFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKQRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1055..1268
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1522..2556
                     /locus_tag="JD016_14730"
     CDS             1522..2556
                     /locus_tag="JD016_14730"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAU5000886.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2597..3814
                     /gene="argJ"
                     /locus_tag="JD016_14735"
     CDS             2597..3814
                     /gene="argJ"
                     /locus_tag="JD016_14735"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HAU5000887.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3829..4689
                     /gene="argB"
                     /locus_tag="JD016_14740"
     CDS             3829..4689
                     /gene="argB"
                     /locus_tag="JD016_14740"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAU5000888.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4719..5906
                     /locus_tag="JD016_14745"
     CDS             4719..5906
                     /locus_tag="JD016_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HAU5000889.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLINKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6015..7037
                     /gene="argF"
                     /locus_tag="JD016_14750"
     CDS             6015..7037
                     /gene="argF"
                     /locus_tag="JD016_14750"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAU5000890.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLV"
     gene            7164..7781
                     /locus_tag="JD016_14755"
     CDS             7164..7781
                     /locus_tag="JD016_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HAU5000891.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSIKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(8135..>8609)
                     /locus_tag="JD016_14760"
     CDS             complement(8135..>8609)
                     /locus_tag="JD016_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAU5000892.1"
                     /translation="KQLINVDHQLLKIADVGYYVETPKTKSGNRVIPMSEKVLEAFQR
                     VLNKRKYAQPVILEGYTKFLFLNRNGLPKVAVNYESMFRGLVRKYTKTQKVALPKVMT
                     PHTLRHTFCTTLANAGMNPKALQYIMGHSNINMTLNYYAHTTSETSITEMKRLIA"
BASE COUNT         3389 a          890 c         1618 g         2712 t
ORIGIN      
        1 caaaatatta agataaaaat atatagaata tatttaaaaa ttattatgca attagaaaag
       61 gagataagta tgcagattat tgatataaat ataaataata ttgacaatga acatatatgt
      121 tgttcaattt ctgaaaaaaa aggagaaaat tgtgtttttt ctaaaaaagc ttggatgaaa
      181 aataattttg ataaaggtct agtctttaaa cgatttgatg taagaggtaa agcttttata
      241 gaatatattc cagcagaaaa tgcatggtgt cctattactg cggacaatta tatatttatt
      301 aactgttttt ggatatctgg aaaatttaag ggtcaaggat attctaatct attgctacaa
      361 gagtgtgttg atgatgctaa atcaaaaggt aaatatggat tagttgtgct ttcgtctagt
      421 aaaaaaatgc cttttttatc tgatgctaaa tatctaaaat ataagggctt ttgtttagca
      481 gatacatcag aaccaaatta tgagctatta tattttcctt ttactgataa tgctcctatt
      541 ccatgtttta agaattgtgt aaaacaaaga aaattaaatg agaatggtat agttttatat
      601 tacagtaatc agtgtccaca tacggaaaaa tatgctccta ttttgaaaaa aattgcaaat
      661 tctagaggta ttgatgtaaa tcttataaaa ttcgaaacag caaaagaagc acaaaatgct
      721 cctgcaccat ttactacata cagtcttttt tttaatggtg aatttgtaac caacgagata
      781 ctttctgaga aaaagattga gaagttttta gataccaaca ttaaaaaatt taactaaaaa
      841 gagatgtaaa tattgtattt tgaaaatata ttattttata atttaatata ttttggatgt
      901 tttttaaatt gacgaatgaa atataaaaat tatatcgcaa tttagataca aaatgctgga
      961 aattaaccat atagaaaata tttccaaaaa tttaaaaatt atattgacaa agttaaaaaa
     1021 ataaactatc atataaaaca ataagaaaat aaattgaaat cctgtgaaaa agagagtagc
     1081 ataaagctag ttgatagaga gctgggaatg gtggaagcct agtaacgaat tttattgtga
     1141 atagagcttt ggaggagatt accagaagta atagtatggt aattggtgaa tctcgcctta
     1201 agagattaga gtgggtaaga ttatcttatc aattagagtg gtaacgcggg tgtatattcg
     1261 tctcttggtc tttgaccaag aggctttttt aattttaaat gtacattttg tagatacttt
     1321 ttaatttgag ttttaatgat tattgtaatt ttaccaataa gagtggtacc gcggaaaatc
     1381 gcctcttagt atttataatg ctaaggggtt ttttgtttta attagagtat tttaatctac
     1441 gtattatcaa atgattatca tatagtaaag tggaaagata ggattttaag actatttgaa
     1501 tataaaaatg gagggaacaa aatgaataaa ataaaagttg gtattattgg tgcaactgga
     1561 tatgtcgggg cagagttgat tagattatta atgaatcatg ataaagtcga agtgacagcg
     1621 ataggttcaa attcgtatgt tggaaaagat atagttgata tatatccaag tataggatat
     1681 aaaaataaca tgatatgtat agaaaatgaa aaagttatag atatgtgtga tgttgtattt
     1741 acagcccttc cacatggagt tagtgaaaag tttgtaataa aggcaattaa atcgaaaaag
     1801 aaagttatag atttaggtgc tgactttaga ataaaagatg aagaagtata tagtaaatgg
     1861 tatggagtat cttttataga taaaatctta cataaaaaag ctgtatatgg attaagtgaa
     1921 atatacaaag aagatataaa agatgcagat ataattgcaa atcctggatg ttatccaact
     1981 agtatttctt taccattgat gccattacta agttctaaat tgataaaaaa taataatata
     2041 ataattgatt ctaaatcagg tcttacaggt gctggtagag aattatcgat tagtagtcac
     2101 tttacggagg ttaatgaaaa tatcactgca tataaaatag ggaaacacag acatactcca
     2161 gaaatagaac aaaatttaag tgaatcctgc aaagaaaaag taagtgtagt ttttacacca
     2221 aacttaatcc cagtaaatag gggaatctta tcaactatat attgtacaaa agaagataat
     2281 atttctataa atgatataca taggaaatta actgattatt atgaatttaa agaatttata
     2341 gaaattcttc cattagataa ggtagcttct ttaaagaatg ttagattctc aaataaatgt
     2401 gtaatatcat tacatgaaaa tggagataca ttgataatct gttcagctat agataatatg
     2461 attaagggag cagcaggtca agctatacaa aacatgaata taatatttgg tattgaagaa
     2521 aatacaggtt taaaaaatat tgcaccatca ttttagaaat aaaaaatact aaactcatat
     2581 aattaggagg ataaatatgg agattataaa gggtggagtt acagtttcag aaggattttt
     2641 tgcttcaggg attcactgtg gcttgagaaa aaataaagaa aaaagagatt tagctttggt
     2701 ttattcagat gttttatgtg atgcagcagc agtagtaact caaaataaag taaagggcaa
     2761 tcctgtttat gttacacagg aacacattaa aaatggaaaa gcacaagcta tcatagtaaa
     2821 tagtgctaat gctaatacct ttaatggaaa agaaggactt ataaatgctt ataaaatggc
     2881 taaattcacc agtgataaat taaatattaa agaaagtgat gttttggttg catctacagg
     2941 tgttatagga aagccattaa atatagattt aatagaagaa aatatggatg aattagtgaa
     3001 taatctaagt aaacaaggtc atattggagc aagagaagct attatgacaa ctgatattat
     3061 aaaaaaagaa atagcagtag caataatgta tggagataaa aaaataacta ttggaggtat
     3121 ggcaaaaggt tctggcatga tacatcctaa tatggcaaca acgttaggat ttataactac
     3181 agatgcaaat attgatggag ttttgctaaa agaagcttta aaaattgctg ttgacaaaag
     3241 ttttaataga gttagtgtag atggagatac aagtactaat gatatggtaa tgattctagc
     3301 aaatggaaaa gcaaaaaatg atagaataaa caaaaaagat gaacattatc aagtattttt
     3361 gagtgcactt acatatgtct gcatagaact tgctaaactt gttgcaaaag atggagaagg
     3421 atgtacaaaa ctaattgaat gctatataaa tggagcatta agtgaagaac atgcagtaaa
     3481 attagcaaaa acagttatat cttcttcatt agtaaagaca gctgtatttg gagctgacgc
     3541 aaattggggt agaatactgt gtgctttagg ttatgcaggt gaagaaatag atatggaaaa
     3601 agttgatatt atttttgaga gtatgaaagg atatattgaa gtatgtaaaa atggaaatgg
     3661 attagatttt aatgaggaaa aagcaaaaaa aatccttgaa gatgacgaaa taagtatatt
     3721 agtagattta aatatgggca atgccagagg aaatgcatgg ggatgtgatt taagctatga
     3781 ttatgtaaga ataaatggaa gttatagaaa ttaggaggat atactttaat gattaatata
     3841 gaaaaagcaa atacacttat agaagcttta ccatatatag aaaaacatca gggaaagact
     3901 attgtagtta agtatggtgg aagtgcaatg aaaaaagatg gtctaaaaga atctgttatg
     3961 gaagaccttg tgttaatgag ttatgttgga atcaatatag ttttagtaca tggtggaggg
     4021 gcagaaatca ataagatgct agcaaaagtg gatatagaaa gtaaatttgt aaatggtctt
     4081 agatatacag atgaagagac catggaaatt gtaaaaatgg tacttgctgg aaaggttaat
     4141 aaagacttag taaataaaat acatacaaaa ggtggaaaag cagtagggct ttgtggaata
     4201 gataataata tgattttatg tgacccatat aaaaattatg aacttggatt tgtaggagaa
     4261 atcaaaaaag taaatgttga gcttatagaa tcttgtctaa agtcaggtta tatttcagta
     4321 attgcaacta taggtgttgg agatgatgga gagacttata atataaatgg tgacacagca
     4381 gcttctgcta tagctaaaga gttaaatgca gataaattga tacttcttac agatgtccct
     4441 ggtttgttaa gagagccaga tgaagaaaaa tcattaataa cagaagttat attagaggat
     4501 gttgataaat tatttgaaga aggtataatt actggaggta tgatacctaa aatagaggga
     4561 tgcgtagatg ccttaaataa cggagtaaat agggtacata ttttggatgg aagagttcca
     4621 cattctataa taacagagct atttactgat agtggaatag gtacattaat aagaaaagaa
     4681 aatgaataaa attataagtt taagagggga gaataagaat gaataacgac aatacaattt
     4741 caaaatggaa tgaatatttt atagatacat ataaccaacc caattttgtc atagattatg
     4801 gtgaaggaag ttgttttttt gatactaatg gcaataaata tatagatttt acaagtggat
     4861 atggagtatc ttctcttggg tatagtaata gtaatttaaa gaatgctcta aaagagcaag
     4921 tagataaatt attacatact tcaaatttat attttaatga gccagtgttg tattcaggag
     4981 agaaaataat taattcaagt ggaatggcta aagtatattt ttgtaattct ggaacagaag
     5041 caaatgaaac agcattcaaa atagcaagaa agtatagtag tgacaaatat ggtaatggta
     5101 gaggaactat cataagttta aaagactctt ttcatggtag aacaatgatg tctttaatgg
     5161 caactggtat ggacaaatac cataagtatt tttatcctct tcccgaaggt tttaaatata
     5221 tagaaagaaa taatattgaa gatttaaaaa ataatcttga ttccacagtt tgtgcaataa
     5281 ttcttgaagc gattcaaggc gaaggtggag ttaatgttct tgaaaaagat tatgttttgg
     5341 aaatagtaaa aatatgtcaa gaaaaggata ttgtagtgat ttttgatgaa gtccaatgcg
     5401 gtataggaag aactggtaaa ctttttggct atgaatattt tgatgtaaaa ccagatatag
     5461 ttaccgtagc aaaagggttg ggagcaggaa taccagtagg aggagtattg ataaataaaa
     5521 aactttcaaa agtactagga aaaggagacc aaggtacaac ttttggaggt aatttattag
     5581 ctatggtggc agcttcagtt gtattagatg agatatctaa agatggtttt tataatgaag
     5641 tattagaaaa aggaaattat ataagaaaat ctattgaaag ttttaataac aaagttgtat
     5701 taaagacaaa aggtattggt ctaatgatag gaattgaaac taatattgaa tctagtatta
     5761 tagaagagaa agctagaaaa aaaggattgc ttattttgac tgctggaaaa aatgtattga
     5821 gatttttacc accattaaca atcagttata aggaaataga tgaggctctt gaaatcctaa
     5881 aagatatatt actagaaatt aactaaatat tcaaaaaata gttgattaaa gataaaaatt
     5941 agaagtaaat aaaaaagtaa agtgctaaca agtttaattg aatttaaaaa gtttataaga
     6001 aggggcgaat taatatgaat aatataagtt taaaaggtaa gagttttttg acattaaagg
     6061 attttacaaa agaagagatt agatatttat taactttatc aagttattta aaaactaaga
     6121 aaaaagttgg tataaaaggc gatttgctac aaggaaaaaa tatagcttta ttatttgaga
     6181 aaacatcaac aagaactaga tgttcatttg aagtagcagc tcatgatgaa ggagcacatg
     6241 taacttatct aggtccaaat gattgtcata tgggtaaaaa agagtctgta tcagatacag
     6301 caaaagttct aggaagattc tatgatggaa tagaatttag aggctttaaa caagaaacag
     6361 ttgaaagtct agcaaaatat tctggtgtac ctgtttggaa tggacttaca gatgaatatc
     6421 atccaacaca aatactggca gattttttaa cagtaattga aaatgtagat aaagatttga
     6481 ataaagttaa atttgtatat gttggtgatg ctagaaataa tatgggaaac tcactgatga
     6541 taggatgtgc taaaatggga atacatttcg tagcactagc acctaaggaa ctttggccaa
     6601 atgaagaatt ggttaaggaa atgaaggaaa tagccaagaa atccaaagga gatataatgt
     6661 tgactgaaaa tattgatgat gttaaaggtg cagatgttat atatacagat gtatgggttt
     6721 ctatgggaga agaggagcaa tatgaagcaa gaataaatca acttaaagat tatcaagtaa
     6781 atatggatat gataaagaaa actgaaaatg aaaatgttat atttttacac tgtcttccag
     6841 catttcatga tttagaaact aaggttgctc aggagattta tgaaaagttc ggattatcag
     6901 aattagaggt tacaaacgag gtatttaatt caaaatattc aaaggtattt gaagaagctg
     6961 aaaatagaat gcatacaata aaagctgtta tggtttctac tatgactgat gtaaatatga
     7021 acaataattt agtatagata tgttttaata tagactttac tttaatgaaa atttataaaa
     7081 tattttaaat atattattca aaaactctca ttatatgtta aaataactta tgtatcaaca
     7141 aattaagagg gaggatttga gatatgaaaa aaatcatatt agctttaaca acttttttat
     7201 tagcaatatc tttaacagca tgttcaagtg caaaaccaga agatacaatt gatagttttt
     7261 ttaacagtgc gaaaaagttt gactttgaag gcatgaataa ggtaatggaa aataatgacg
     7321 aaaaatataa ggatatatta aaggaattag atacaaaaga cccaaatgcc caatatgttt
     7381 tagattatct aaaacagaat gcatctaaaa tcacatatac aataaaagac agcgaggtta
     7441 aaggtgataa ggctacaatc aaagtagaat gtaaatttat agattcaaca ccattacttc
     7501 aagaaatagt agctgaggca tttacaaaga tgattggtat gagtttttca ggacaagact
     7561 taactgatga gaaaacaact gaaatgttag tatcaattat gaaagaaaaa caaaaaagca
     7621 ttaaagagac ttatgttaca aaaaatgtag agtttgaatg ttctaaaaag gataataaat
     7681 ggataataag ttcagtaaat gatgcagtag ctgatgtatt gttatctaat cttgttacag
     7741 caggacaaga attttctaac tctatgaata cacttgaata atattgttta ggtgtagtaa
     7801 agtaataaat atatgattat ttttataaat aaacatataa atatttatga aggataccat
     7861 ttttggtatc ctttttatta atataatgaa atacatggtt aatttctata ttgtcaagaa
     7921 actatgatat atgttataag tctatactat ttaagataaa tcacaaagta atttttgact
     7981 atcttttcaa aagttcttat atgccggtat agcctattgt tacaggcttt tccggcattt
     8041 ttcgttatgg aagatattct caaatcttct tatatccccg tatgttttgg tgttcaaaag
     8101 tagtatgtgg agtagtaaat tctccgttta ctacttatgc aatcagccgc ttcatttctg
     8161 ttatcgacgt ttcggaagtc gtatgcgcgt aatagttcag cgtcatattg atgttggaat
     8221 gtcccatgat gtactgcaat gcctttgggt tcattcctgc atttgctaac gtggtgcaga
     8281 atgtgtggcg taaggtgtgc ggtgtcatta cctttggcaa tgctaccttt tgcgttttgg
     8341 tgtatttcct tacaagcccc cggaacatac tttcatagtt gactgccact tttggcaagc
     8401 cgttccggtt aagaaacaga aactttgtgt agccctctaa aataacaggc tgtgcatatt
     8461 tccgtttgtt cagcacccgt tgaaatgctt ccaacacttt ttcactcata ggaataaccc
     8521 gattgccgct tttcgtctta ggcgtttcca catagtaacc cacgtctgca atcttcaaaa
     8581 gttggtggtc tacattgata agctgtttg
//
LOCUS       DACXMZ010000173         8536 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.174, whole genome shotgun sequence.
ACCESSION   DACXMZ010000173 DACXMZ010000000
VERSION     DACXMZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.174"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            134..1993
                     /locus_tag="JD016_14765"
     CDS             134..1993
                     /locus_tag="JD016_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAU5000893.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2285..2884)
                     /locus_tag="JD016_14770"
     CDS             complement(2285..2884)
                     /locus_tag="JD016_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tellurium resistance"
                     /protein_id="HAU5000894.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYTMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2987..3784)
                     /locus_tag="JD016_14775"
     CDS             complement(2987..3784)
                     /locus_tag="JD016_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAU5000895.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3897..4002)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4094..5080)
                     /locus_tag="JD016_14780"
     CDS             complement(4094..5080)
                     /locus_tag="JD016_14780"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAU5000896.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5564..7294
                     /locus_tag="JD016_14785"
     CDS             5564..7294
                     /locus_tag="JD016_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HAU5000897.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7404..7484
                     /locus_tag="JD016_14790"
     tRNA            7404..7484
                     /locus_tag="JD016_14790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7438..7440,aa:Leu,seq:tag)
     gene            7819..8334
                     /locus_tag="JD016_14795"
     CDS             7819..8334
                     /locus_tag="JD016_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HAU5000898.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
BASE COUNT         3073 a         1061 c         1311 g         3091 t
ORIGIN      
        1 aactatgtaa aacaaggttt atatattcta atagtataga aaaatttatt gtaaacgcat
       61 gtatattatt agaaaaaata gtttgaaggt attcaataaa cataaaaaac aatatagggg
      121 ataaaggtgt tttatgaaga gatttttgag gagaattatc ttagttttat ttattttgtt
      181 gatttttata agttttattt ctattaaatt aattcataat gttggagatt atggaaaatt
      241 gattaattat gttgggattg ttcgtggagc tagccaacgt ctaactaaac tagagatgaa
      301 tcatcaacca aatgatgaat taatagaata tattgatgaa atcctacagg agttgattac
      361 agggcatgga gattatggtt tagtaatcac tgattgtaat gaatacaatg aggacttact
      421 tttattagaa aaaaagtggg aagatttaaa tattgaaatt aaaaaagtaa gaatgaaaga
      481 acagaataac cagttattaa gtataagtga agagtttttt tctcttgcaa atgatacagt
      541 gtttaaaata gaaaactttt ctaaagaaaa aagtaattac ttaatgactt taataataat
      601 tatatctatt ataggtatat tagcatgtat catattgatt ttacaatata gtaaaaaaat
      661 ggttagactg gaaaaattaa atgtagattt aaaaaatatt gcatataaag atgaactaac
      721 aggagtaaat actatagaaa aatttaaatt ggatgcaaat caaaatattt gtatacataa
      781 ggataagaaa tttgctgtat tctatattga ttttgaaaac tttaaataca ttaatgatat
      841 atttggatat gattatggtg acatgatact gaaaagatat gctaatctta tgatgaatga
      901 catagggaaa tatgaaatat ttgcaagaga aattgcagat agatttgttg cgttaagatg
      961 ctatgtagat aaggaagatt tagtggtaag acaacgagat gtagacaatg aactaattaa
     1021 tataacaaat gaaattaaaa ataaacatag tattactgtt gttagtggaa tttgttgtat
     1081 tgaagatgta aatgaaaaat taagtataga tggtttaata aatagagcaa attttgcaca
     1141 aaaaactgtt aagaataaac caggtactaa ttatgctttt tataatgaca gtattcgtaa
     1201 aaaaatgatt gaagaaaata ctatcaagag tagaatacat gaagctattg aaaaaagaga
     1261 atttatagtt tatttacagc ctaaagttaa tttacatagt caaaaaataa attgtgcaga
     1321 agctttagtt cgttggctta ctccggataa aggtattata tcgccagcaa tatttattcc
     1381 agtacttgag aagaattttt ttattgcctt agtagataag tatgtttttg aggaggtttg
     1441 taaatggatt agaaagaggt tagatgaaaa taaacctttt gttcagatat cagttaatgt
     1501 ttcaagaata caattttata acactaaatt tgtagaaact tattccaata tacaaaataa
     1561 atacaggata cctaaaaata ctatagaaat tgaatttaca gagagtgttg cttttgagaa
     1621 tcaaaaacat cttttggaaa ttatacatga tttgcatgaa aatggattta cttgttcttt
     1681 agatgatttt ggaaaaggtt attcgtcttt aagtgtttta aaagatttac catttgatgt
     1741 tttaaagctt gattctatgt ttttcaaggc tagtttagat aaagataaag agaaaatagt
     1801 aataaaaaat attgtacata tgttaaaaga acttaatatc acaactgttg ctgagggcat
     1861 tgaatgcaag gaacaagttg aatttcttag ggatattggg tgtgatttag ttcagggatt
     1921 tgtattttat aaaccaatgc caatattaga atttgaaaaa atattggata aagagtttgt
     1981 ttataatagc taaaatgtac tttataaaat atgaactaat taagtaaaat tattttgtac
     2041 tgaataaatt tttactttat ttacagaata atgattattt acagaaaagc gcgaaaagtg
     2101 tattccttta agtgtgggat ggatagcaca aataggggag aaaaaatatt ggccatataa
     2161 gaatgcataa taatataaat atttgaaagg tcagcattat ataaaaatta aaaagaaaat
     2221 aaataatttt tctataaaaa agagactatg agaaaagtta ctcatagtct cctaaataat
     2281 gtatttattt tcttcctgca acccatttta atccccaatg aaattcagta tccatttctc
     2341 tatgaccttt gaagtattta actacttttt caacactgaa tgtctcatca tttacatttt
     2401 ctatcaatgc tattgaacac atagtatagc catttttatg ttcatcaagt ttgacaacta
     2461 agtctgaatc ttgtttttgt ttgattgtta caattccatc agcttcagac cagttagcta
     2521 caccttcgta tataaaagca taaataagta ttctttttat atcttttatt ttattaccat
     2581 ttattcttaa attttctcct tgtgtattac ttcctgttct gtcatctcca tctaattgag
     2641 caaaaggtgg attgtctaaa ctaccaaagg catttcctaa tgcttgaaca gcacctttta
     2701 caccattttt catctcaaat aggcaaccca aatctaaatc tatattactg cttcttaaag
     2761 agccaaaaaa accctttttt tgtacttttt gattccaatt taaattaact aagatttcac
     2821 ctaaatcgct attttccttt tttgtaaggt ttattttttg tccctttttt aattctatag
     2881 ccatagtaat cttcctccac aaacatagat ttatttaaat ggtagcactt aataagtcaa
     2941 tattcaacaa gattagaata ataatattaa aattatacta aatatactaa aaagtaggaa
     3001 tcacaatacc tttgtatttt tcttcaatta agcttcttgc tttatctgat gtaagaacat
     3061 cttgtagaac tttaatcttt tcactgtctt tatcttcagt tctaaatgct aaaagatttg
     3121 catactcgtc atttatagga gctgtataaa ttccgttttt cttggcatca agaccagcag
     3181 gtacagcata agtagtatct ataaatgcag cagtcacatc tggaagagaa gatggaattt
     3241 gagcaatatc taactctttg aattgtattc cctttggatt ttctattatg tccttaggac
     3301 taactaatcc atccccttta ggtaacgtta aaagtccaat actttcaagg tatctaaggt
     3361 tcttagaaca agaagatggg ttattgctta ttgcaattgt atctcctttt ttaaattcat
     3421 caacagattt tatcttataa gagtaaagta ttgctgggca tacatatagt tttttaccag
     3481 ccattatttt gtaacctttg gccttaacag cttcttttag ataaggttca tgttgaaata
     3541 gatttgcatc aatttcacca ttatttaaag cttcgtttgg aagtatgtaa tcatcaaagt
     3601 ttttaacttc taaagtatat cctttttctt taataagtgg ttttaattct tctaataact
     3661 ctcctcctgg aactaaagta gcaccaacaa caattttttt atcatcttta gctttagaac
     3721 atccaaccac agctaaagtt aatactagac ataataacaa acataataat tttttgaatt
     3781 tcatataaat tccctcctga atataattga ttagttaaaa ataaaatacc attctttatc
     3841 acactgcaaa ataaaaataa gcctgagttt aaactcaggc tttaaaagct aatttagact
     3901 catctttcag tataatactg caggatttag caccaatgta tataaaaata tactggttgc
     3961 tgggtttcat agggccagtc ccttcaccac tctggataag aagtatcaat ttttaattat
     4021 gttataaata ttatcatgat tttttacgtc tgtaaacata attttataaa tattttaaaa
     4081 tctttaatgt attttattta acacctaaaa ttccagaaac tagaatatca gtatcagcac
     4141 ctaagaaata gtcatttatt tcaatagaat ccagagtatt tctcaaagca ttttcttcat
     4201 gccttatacc aataagttta ttttctacaa atgcaacatc acattttcca aaaaaatctc
     4261 caaaaaattt tatactagtt attatgcctt tatcaacatt taaattaaac tcaacatttc
     4321 caccagaata ttttaattca tttgaaagag tatattttgg tgaattacca aaattccaat
     4381 cccaagtttc gtattttgat ttagtaagct tttctatttg ttttatatca tgctcactca
     4441 aacaataatt agtattatca gtatttgttt tagaaagata atccattata gcatctttaa
     4501 attctaatat atccattttt gaatgtaaat gttcacttat attggttaca cgagatttta
     4561 cagattttat tccttttcct tcaaatttta taggtttaac ctttaatgct tttgagaggt
     4621 catttatttc agatgaaaat aaaagagtcc catgatgcat tactttgttt ttgtagttgt
     4681 attgagcatt tccagaaaat ttcttgccat ctataagaag gtcatttctt ccagaaaatt
     4741 cggcattgac atctagtgtt ttaagtaaat ctattatggg ctgagtgaat cttctgaaat
     4801 cactaaaatt attattgtta caagctataa atgtaaaatt tatatttccc aaatcatgaa
     4861 atacagctcc accaccagac tgtctcctaa cgacaggtat agaatattct tttatatatt
     4921 ctaaatttat ttcagaaagt gtgttttggt tttttccaac aattatagaa ggttcattcc
     4981 tccataaaat aaataagtct tcatcaaaat tttttagaag atattcttcc attgctaaat
     5041 taaaataagg gtttgttttt tcattataaa ttaataacat taaagtagtt cctccataga
     5101 attgataaaa tttttattat gaatacaatt aattttataa ttcgacaata atataaaaac
     5161 tcctttaaat tagaaatttt aaaaactacc ttgcaaagtt taaataaata atatatactc
     5221 aaaatatgtt taataaaagt gaatagaagg gtagaggcgc aataatctat cagtactaat
     5281 aaggagttaa gcacagagag ttattaggaa aggagatatt gccgaagttt tataatcgat
     5341 gctttaagat tatattgctg ggggtacata gaaaatatgt acaactgtca caggtttatt
     5401 tgtggtgagc tatcatttaa ggaattatat tatatttatt tttactattg ttgatatatt
     5461 gtaatgttta agccttgagt gtttaccact caaggctttt ttagttttat tgtaacataa
     5521 taccctgtac agttcacaaa attaaaagga gagggagatt tatatgagga acaaaaaaat
     5581 taatattatt tttctgacga caatcatgtt cataatgtca acagtaatgg tttttgcaga
     5641 agaagatata gacactattg cactagccaa tgcagaaaaa tttgggattt taactttaat
     5701 accaccatta gttgctataa tacttgcatt tataactaaa aacgtaataa tttctttact
     5761 tataggtatt ctttctggta gttttataat taaagctagt ggtattaatg tttttgcgac
     5821 atttatacag gcatttttag atttagtaga cagagcttta gtatctttag cagacccttg
     5881 gaatgcaggg ataatactac aggttttggc aattggagga gtaataaatc ttgttgctaa
     5941 aatgggagga gctaaagcta tagctgaagc acttgctaaa agagcaaagt cagcaaaagg
     6001 gacacagctt ataacatggt ttttggggct tttagtattc tttgatgatt atgccaattc
     6061 actaatagta ggtcctatga tgagacctgt tgcagataaa atgaaaatat cgagagaaaa
     6121 gctggcattt ataatagatg ccacagcagc accagttgct ggtttagcta taatatctac
     6181 atggataggt cttgaggttg gtttaataca tgatgctttt gaaagtataa gtatagatgt
     6241 agatgcattt ggtatatttt taaatacaat accatttaga ttttacaata tattaatatt
     6301 agctttcatt gtaatatcag cattattatt aaaagaattt ggtccaatga gaaaagctga
     6361 gataaaatct agaagtagaa aaattagtat agaccttgat gaaggcgtag aagagttgga
     6421 tgatttagct ccaaaaaatg gagtaaaatt gagtgtatgg aatgctataa tcccaatagg
     6481 aacattgata atagttgcat tagcatcatt ttattatagt ggatatacat ctataatggg
     6541 aggagacgat aaggctctga tacaactatt tacaaattct ccatattctt ttgaagcaat
     6601 aaaagaagca tttagcgcat ctgatgcatc tagagcatta ttccagtctg cacttgttgc
     6661 aagtttagtt gctattataa tggctgtagt aaaaaagatt tttacaatat cagaagcaat
     6721 tgatgtttgg atagatggaa tgaaaagttt agtaatcact ggtgtaatat taatacttgc
     6781 atggtcatta agttcagtaa taaaagagtt aggtacagct aaatttttaa tacacctatt
     6841 atcaggttca ctaccaccat tcttattacc aagtttaata tttggtttag gagcaataat
     6901 atcttttgct actggaacag cttatggaac aatgggtata cttatgccac ttgcaattcc
     6961 attagcatac tcattaaatc ctgatatgtc ttatgtaata gtaagtacta gtgcagtttt
     7021 aacaggtgca atatttggag accattgttc tccaatttca gatacaacaa tactttcttc
     7081 aatgggagct ggctgtaatc atatagacca tgttaacact caaatgccat atgctatatt
     7141 tacagcagta atcactattg tatttgggta tataccagct ggacttggat tgccaatata
     7201 tatagtatta ccagtggcaa tagctgctat atttgtaggt attcaaataa ttggtaagaa
     7261 agttgatgaa gcggaaatag aacttgtaga ataaagatat aaataagttt caccttttat
     7321 tatcactatt ttgttagttt ttagataatt ttaaaaagtt attgaaaata atggcgaaat
     7381 atagtaaaat aaaagagtga taagcggatg tggcggaatt ggcagacgca ctagacttag
     7441 gatctagcgc cattggcgtg ggggttcgac tcccttcatc cgcactttac agtacttaat
     7501 tgtactgaaa aaaactgggg ttgctggctt tcggagtggg ctatcccgtt tttatttttt
     7561 tgtatatata atagattggc actttagtat aaggtgcttt ttttataggc aaaaacagta
     7621 catttatttc tgttttatta tcattcatct attagaatat actttagtta agaatgtgtt
     7681 aataagcagt gttcacattc ttgataaaat gtggaataat atgtattttt actaagacgg
     7741 aaatgacata attaaaccat taaataatat tataagatga tgaataagta gataaatcaa
     7801 attttgtagg aggatattat ggaaaaatat atatggttgt ttccattact ttttattttt
     7861 catgatatgg aagagataat aggttttgga atatggctta aaaaaaataa atcaatgctt
     7921 gataagaagt atccatttat cagtaaagtt tatgaaaatt atagtactga aggcatggca
     7981 tttgctgtgt ttgaagaatt tattttatgc attattttct gtatacttac agttataaca
     8041 gagaatcagt atgtatatct actatggtta ggtagtttta tagcatatac tttacatttg
     8101 gtaattcata tagggcagtc aatcataata agaaaatata taccttcact tattaccagt
     8161 atcatatgct taccaataag catttggtgt ataagtaaga gtatttatat agttgattgt
     8221 gaaatgagta caacaattct gtacagtata attggcatta taattgtagc attgaattta
     8281 aagtttgcac agtcgctgat tggaaaattt acaaagtgga tgtcaaacat ataaatttta
     8341 gtttattgga cagttcaagg aatcagaaat tggaatttta tagtattaat attttatggt
     8401 attaatataa tgtataaaat tttatagcaa tgtatttcat aagtataaag tgactagaat
     8461 tttaagaaat atacaaattc tataaaaact aaataatttc aagtatattt taaaaatctt
     8521 atttttcaca agttta
//
LOCUS       DACXMZ010000174         8480 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.175, whole genome shotgun sequence.
ACCESSION   DACXMZ010000174 DACXMZ010000000
VERSION     DACXMZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.175"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(44..265)
                     /locus_tag="JD016_14800"
     CDS             complement(44..265)
                     /locus_tag="JD016_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000899.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSIENLKKKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMAEYFEDEGVEV"
     gene            complement(283..972)
                     /locus_tag="JD016_14805"
     CDS             complement(283..972)
                     /locus_tag="JD016_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000900.1"
                     /translation="MDLNKIMNDALVELEENGFVEEVVKKQLAETIKRVVNEVFGNWG
                     DFSKKIEKHLSENININLDKLDIQKYNVLVATTVKEKIDMTMKVEAIEHLKRNLDNML
                     VGAEKEYKMSELLEILKMDKYDIDEYEGYEDCITFILDENKYGSGWIEIDKEPNKSKY
                     RCEHSILLRKDGTIASYRYENREISSKDIMSGFDNFGDLLFKIFAHGSKIILDLGNDI
                     EDYDLTNGEDY"
     gene            complement(1265..1381)
                     /locus_tag="JD016_14810"
     CDS             complement(1265..1381)
                     /locus_tag="JD016_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000901.1"
                     /translation="MNILASLILIVGSFIGGRVYEYRLNLKECENCDNKRGV"
     gene            complement(1365..1790)
                     /locus_tag="JD016_14815"
     CDS             complement(1365..1790)
                     /locus_tag="JD016_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000902.1"
                     /translation="MSLIKYRGYDFENEKWSDAVDCLFMLKENCEWQRVCNVGVFSKC
                     WSGNNEEIYEGDVLKEPYNTKNKYEYGIVRQENYALELYVEWHYLKQFEGKWEEITSK
                     ATIMNSKKYVVVGNECENLEEIKKEFLERKEMLENEHLG"
     gene            complement(1908..2273)
                     /locus_tag="JD016_14820"
     CDS             complement(1908..2273)
                     /locus_tag="JD016_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000903.1"
                     /translation="MLKVEKYFNGSVADNIFEDDLTLRNYLALYCCVHGVRKNGELVF
                     PTSEKMLIEFNVDKNRKKKKKGSKVKLINAKTGEEKIFDSIDSAACFLRLQSQAVYQT
                     IKKKTKTRSGWKAEYIKEE"
     gene            complement(2287..2544)
                     /locus_tag="JD016_14825"
     CDS             complement(2287..2544)
                     /locus_tag="JD016_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000904.1"
                     /translation="MIIIRSQDKTDLVKADWINVDKEYVYAMFGEINNFKEIGKYEDE
                     KRAMQVLDNIQRFIEGGTRTDSIDSYKVRSYRNRIFQMPVK"
     gene            complement(2711..3649)
                     /locus_tag="JD016_14830"
     CDS             complement(2711..3649)
                     /locus_tag="JD016_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAU5000905.1"
                     /translation="MNEDKSVIEKLNILSGGYGLMPRIIARDRWLTVGARMLYSYLTS
                     FAGNDGTCFPSRDLICYELDISKDTFTKYKKELEMSGYIRVHKNKSKQGKMQNNIYEI
                     VFDRTYIDECISKRGLKEEKKKKKPCTKKQDTEPYPKNVDMEPCPTFPDTTQPDTENM
                     DTNSNSINSNSINNMYIGKQPVDNFLKEFKKLYEENIGVIYPVTAEWLLEVSNEVDIR
                     VFKRAIEICAEKMNMNLAYLKGILKKWKDANITTYEQLESYRLQQENKKTKKVVNNHV
                     SKNKFANFEQTFTKYSENELDDIIKKSQKEKFGVGS"
     gene            complement(3664..4497)
                     /locus_tag="JD016_14835"
     CDS             complement(3664..4497)
                     /locus_tag="JD016_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAU5000906.1"
                     /translation="MASEKAKGALEKKVSGANTVKVSPSKGMEQLMNKMASQIKKALP
                     SMVSSERFQRVALTAFSNNPRLQSCEPMSFIAAMMESAQLGLEPNTPLGQAYLIPYGN
                     KVQFQIGYKGLLELAQRSGKIKTIYAHKIRENDKFEIKYGLHQDLVHEPKLNGDRGEI
                     IGYYAVYHLDTGGDSFSFMTKEEIIEFAKSKSKSYSSGPWQTDFDSMAKKTVIKQLLK
                     YAPLSIELQKAMVGDETIKSEIDEDMSMVVDESESLEVDFEVKENMDGKVSVEEAINV
                     D"
     gene            complement(4510..5400)
                     /locus_tag="JD016_14840"
     CDS             complement(4510..5400)
                     /locus_tag="JD016_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000907.1"
                     /translation="MDWLKNRQLGIGGSDASAVAGLNPWKTSVQVYIEKKEEIPIETK
                     SFRMELGNRLEGLVAELFTEETGLKVRNVNGMLKNEKYPFAIANIDRAIVGEKAFLEC
                     KTTNSFSIKEWENGVPLHYEIQCLHYMAVTGATHCYIAALLGNEKFVWHKINRDNEVI
                     KNLMKIESEFWEENVLKDILPIPDGSDAYSEFLKTRYKNSVKEKIELNLLEDGISKLK
                     RYDDIVLQMKELKGEKQLIEQEIQSEMREFELATLGGRIITWKGATKRSIDTKRLREE
                     MPDIAEKYTNISSYRTFKIK"
     gene            complement(5595..5813)
                     /locus_tag="JD016_14845"
     CDS             complement(5595..5813)
                     /locus_tag="JD016_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000908.1"
                     /translation="MYMVFNLEKFKVGNAIRISCERFGFEIDCIVVVATEEELNLAYF
                     DEGRGCMEYQALITEDIQDGDYEIKILS"
     gene            complement(5823..6011)
                     /locus_tag="JD016_14850"
     CDS             complement(5823..6011)
                     /locus_tag="JD016_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000909.1"
                     /translation="MKTIYKNKVYKVERNKELYKITYYDEQKSNKKFNKDKKVKRSTL
                     TRDIELVNLYLPVNLKIK"
     gene            complement(6113..6442)
                     /locus_tag="JD016_14855"
     CDS             complement(6113..6442)
                     /locus_tag="JD016_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU5000910.1"
                     /translation="MSVALQFIDTKDLVQELMRRDDTTDIIKMFLDREGIKRMELMTI
                     EEFCEYLKISDVTARNMAREAMITKDFIALKIGRKYMIDRISFEEFIMKNAMKDKDVM
                     KKRKGVI"
     gene            complement(6512..7303)
                     /locus_tag="JD016_14860"
     CDS             complement(6512..7303)
                     /locus_tag="JD016_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAU5000911.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETDARNISNNFNNNKDRFIE
                     GKHYFLLQGDDLKNFKGIHTEYENLKFTSKMYLWTERGANRHCKILDTDKAWEQFDNL
                     EETYFKVKQHKPTCIEDVLIESLKEMKDLRLQVNQANSIALEAKTEVETIKDVVSLDS
                     NSWRTNTHQLIARIAKKQGGFEHINMLRTESYELLNKRFGVDLHRRLINKRRKMAEEG
                     VSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            complement(7296..7532)
                     /locus_tag="JD016_14865"
     CDS             complement(7296..7532)
                     /locus_tag="JD016_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5000912.1"
                     /translation="MNKLKDFRIQKGLSRNQLGAELGLTSRYIAFLENGERVPSLNTA
                     VKIAAYFNTSIEYIFLKNNCTKSTFKEKGAEKYE"
     gene            7677..8042
                     /locus_tag="JD016_14870"
     CDS             7677..8042
                     /locus_tag="JD016_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5000913.1"
                     /translation="MIGYRIKELRKEKDITQKELATFLGLTPKMISFYEKEERFPPHD
                     IILKLSDFFNVSTDYLLGKVNVKNIDNLSELELIENLNFSDDIKEALKLISELSPSSQ
                     EKMFKIAKVFLEEELNEKK"
BASE COUNT         2426 a         1654 c          777 g         3623 t
ORIGIN      
        1 tcgctattct tatctaaaac atgtattata aatttgtgta ttatcatact tccacccctt
       61 catcctcaaa atattcagcc attttttggc ttctagtatc ttttataact tcttcaactt
      121 tatctattgt tacaagcagt atcttgtcat ctttagctaa taattcagct ttctttttta
      181 aattttctat acttccacat gaataattta tcttgcgttc atctagtaat aatccttttt
      241 gccatctcaa tacatacttt gacatttgtt ccatccccta ttttaataat cttctccatt
      301 tgttaaatca taatcttcta tatcatttcc taaatccaaa attatttttg acccatgagc
      361 aaatatttta aatagcaaat ctccaaaatt atcaaatcca ctcattatat ctttagatga
      421 tatttctcta ttctcgtatc tatatgatgc aatagttcca tcttttctca acaatataga
      481 gtgttcgcat ctgtatttac ttttatttgg ttctttatca atttcaatcc atcccgaacc
      541 atatttattt tcatcaagaa taaatgttat gcaatcttca tagccttcat attcatcaat
      601 atcatattta tccattttta atatttccaa caattcgctc attttatatt ctttttcagc
      661 acctacaagc atgttatcta aatttctttt taagtgttca atagcttcta ccttcattgt
      721 catatcaatc ttttctttta ctgtagttgc tacgagcaca ttatattttt gtatatccaa
      781 tttatctaaa tttatattta tattttcact tagatgtttt tcaatttttt tactaaaatc
      841 accccaattt ccaaaaacct cattcacaac tctttttatt gtttctgcca attgtttttt
      901 aactacttcc tctacaaagc cattttcctc taactctact agtgcatcat tcataatttt
      961 atttaaatcc attatattat tcccctctct attttcattt ttgagagtca caaaacactt
     1021 caacaataat ttatactaaa agatattttg taactctcta aactgtctta attagatatt
     1081 ttcacttata tttcttctaa catttcctcg agtttatttt ttaataaatc atatttttct
     1141 ttagtttcta aatctaatat tctaactctt cctcgctctg ctataatagc tatatttgaa
     1201 ctttcacata tcatctgtat ataatttaca gaagcattta tcatttctaa tctatcatcc
     1261 attcttatac acctctttta ttgtcgcaat tttcacactc ttttagattc aatctatact
     1321 cataaactct accacctata aaactaccta ctataagtat taaactagcc aagatgttca
     1381 ttttctaaca tctccttacg ttctaagaac tcttttttaa tttcttccaa attctcgcat
     1441 tcattaccaa ctactacata ttttttgcta ttcattatag ttgctttact tgtaatttcc
     1501 tcccatttac cttcaaattg ctttaaataa tgccattcaa catataattc aagagcataa
     1561 ttttcttgtc ttacaattcc atattcatat ttatttttag tattataagg ttctttcaat
     1621 acatctccct cataaatttc ttcattattt ccagaccaac atttagagaa tactccaaca
     1681 ttacatactc tttgccattc acaattctct tttagcataa ataaacaatc tactgcatca
     1741 ctccacttct cattctcaaa atcataacct ctgtacttaa ttaaactcac ttttaatcat
     1801 ctcctcatat tcttctctag ccttatctat agcaataaat atgtcctctc cattgtcata
     1861 taactctttt gctcttttaa ttgtatattc tgtccttgaa acttccgtta ttcctcctta
     1921 atatattcag ctttccagcc acttcttgtt ttagttttct ttttaattgt ttggtaaact
     1981 gcctgactct gtagtcttaa aaaacatgct gcactatcta tagaatcaaa tattttttct
     2041 tcaccagttt tggcattaat caactttacc tttgaacctt tctttttctt ttttctattt
     2101 ttatcaacat taaactctat tagcattttt tcagaagttg gaaatacaag ctctccattt
     2161 tttcttactc catgaacaca acaatatagt gctaagtagt ttctaagagt caaatcatct
     2221 tcaaatatgt tatctgcaac agaaccatta aaatattttt caacctttaa catttcagct
     2281 ccctccctat ttaactggca tttgaaatat tctatttcta taacttctaa ccttataact
     2341 gtctatagaa tccgttctag tcccaccttc aataaatctt tgtatattat ctaatacctg
     2401 catagctctt ttttcatcct catattttcc tatttcctta aagttgttta tttctccaaa
     2461 catggcatat acatattctt tatcaacatt tatccaatca gctttcacta aatctgtttt
     2521 atcttgactt ctaattatta tcatccctaa taccccccta ccttatttaa cttcaacacc
     2581 ttcaatatca tcaacccaaa gttcttcaca tcttttatag tgttctatac cattgcatgc
     2641 tataaatttc tctttatcga acttataagt tttacctttt tcaaatttca tctttcatcc
     2701 ctccaatatt ttaacttcct actccaaact tctctttttg gcttttctta ataatgtcat
     2761 ctagttcatt ctctgaatac ttagtaaatg tttgttcaaa gttggcaaac ttatttttac
     2821 tcacatggtt attaactact ttttttgttt tcttattttc ttgctgtaat ctatatgatt
     2881 ccagttgctc atatgtagta atatttgcat ccttccattt tttaaggata ccttttaagt
     2941 atgctagatt catattcatc ttttcagcac atatctctat agctctttta aatactctta
     3001 tatctacttc attagatact tctaataacc attcagctgt aactggatat attactccta
     3061 tattttcttc atacagcttc ttaaattctt ttaaaaagtt atccacaggt tgctttccta
     3121 tatacatatt attaatactg ttactattaa tactgttact attagtgtcc atattttccg
     3181 tgtccggctg agtcgtgtcc ggaaaagtag gacatggttc catgtctaca tttttaggat
     3241 acggttccgt gtcttgcttt ttagtacatg gctttttctt ctttttctcc tcttttaaac
     3301 ctctcttaga aatacattca tctatataag ttctatcaaa tactatttca tatatattgt
     3361 tttgcatctt gccttgtttg gatttattct tatgaaccct tatatagcca ctcatctcta
     3421 gctctttttt gtactttgta aatgtgtctt ttgatatatc tagttcataa caaattaaat
     3481 ccctagatgg aaaacatgtt ccatcattcc ctgcaaaact agttagatat gaatacagca
     3541 ttctagcgcc aactgtcaac cacctatctc ttgctattat tcttggcata agaccgtatc
     3601 caccacttaa tatatttaat ttctctataa ctgacttatc ttcattcaag gtgcctcacc
     3661 tacttaatca acatttatag cttcttccac actaacttta ccatccatat tttcttttac
     3721 ttcgaaatca acttctaaac tttcgctttc atctacgacc atgctcatat cttcatctat
     3781 ttcagatttt attgtttcat cacctaccat agctttttgt aattctatac taagtggtgc
     3841 atattttaat aactgtttta taactgtctt tttagccatt gaatcaaaat ctgtttgcca
     3901 tggtccacta ctataacttt tacttttact ctttgcaaat tctataattt cctctttagt
     3961 cataaaagaa aaactatctc ctcctgtatc caaatgatat actgcataat atccaattat
     4021 ttcccctcta tcaccattta atttaggttc atgaactaag tcttgatgaa gcccatattt
     4081 aatctcaaat ttatcgtttt ctcttatttt atgagcatat atagttttta tctttccact
     4141 tctttgtgct aattctaaaa gtcctttata cccaatttgg aattgcactt tatttccata
     4201 tggtatcaaa tatgcttgac ctaaaggcgt attaggctca agacctaatt gagctgattc
     4261 catcattgct gctataaaac tcataggttc acatgattgt aaccttggat tattactaaa
     4321 agctgttagg gcaactcttt gaaatctctc gcttgaaacc atactaggta aagctttttt
     4381 tatctgactt gccattttat tcataagttg ctccatacct ttacttggac ttaccttgac
     4441 tgtatttgct cctgaaactt tcttttctaa tgctcctttt gctttttcac tagccatata
     4501 tattaccccc tattttattt tgaatgttct gtatgaactt atatttgtat atttttctgc
     4561 tatatcaggc atttcttctc ttaatctctt ggtatcaatg gaccttttag tagctccttt
     4621 ccaagttatt attcttccgc ctaatgtagc taactcaaac tctctcattt cactttgtat
     4681 ttcttgttct attagctgtt tctctccttt tagttctttc atttgtaaaa ctatatcatc
     4741 atatcttttt aactttgata taccatcttc aagtagattt agttctattt tctcttttac
     4801 tgagttttta taccttgttt tcagaaactc actataagca tctgaaccat caggaattgg
     4861 taaaatgtct tttaatacat tttcttccca aaattcactc tctattttca ttagattttt
     4921 aattacttca ttatccctat ttatcttgtg ccatacaaac ttttcatttc caagaagtgc
     4981 tgctatataa caatgtgtag ctcctgtgac agccatatag tgtaagcatt gtatttcata
     5041 atgaagtgga actccatttt cccattcttt tatagaaaaa ctatttgttg tcttacattc
     5101 taaaaatgct ttttctccta ctatagctct gtctatatta gctattgcaa aaggatactt
     5161 ttcatttttc aacattccat ttacattacg gaccttaaga ccagtttctt ctgtaaaaag
     5221 ttctgcaact aatccttcta atctattgcc taattccatt ctgaaacttt tagtttctat
     5281 tggtatttct tctttctttt ctatatatac ttgaacagaa gttttccagg gatttaatcc
     5341 tgctactgct gatgcatcac taccccctat tcctaattgt ctatttttaa gccaatcaat
     5401 tttatctatg ttcttagtat cagttactat aaaagcatct aaatattttc tacgacttga
     5461 aattttattc attttatggt atcctcctaa ataagttgaa ttttttgtat gtgttggtta
     5521 ctttgatcag cacttttttt attgaaataa tcctgctaaa catattgcaa ataaacctac
     5581 cattttattt cctcctaaga taaaatttta atctcataat caccatcttg aatatcttct
     5641 gttattaagg cttgatactc catacagcct ctaccttcat caaagtaagc taaatttagt
     5701 tcctcctcag ttgctactac tacaatacaa tcaatttcaa aaccaaatct ttcacaactt
     5761 attcttatcg catttcccac tttgaacttc tctaaattaa ataccatata caattaaacc
     5821 tcctacttta tttttaaatt tactggtaaa tacaagttaa ctaactctat atctcttgtt
     5881 aatgtacttc ttttaacttt cttatcttta ttaaacttct tgttactttt ctgctcgtca
     5941 tagtatgtaa tcttatataa ctctttgttt ctctctacct tgtaaacttt gtttttatag
     6001 attgttttca tgatgttatc ccctcgttgt attttctaag accttcaaaa cttgctttta
     6061 tttgcttatc attgcaaaat tggatataag ctatcagtac tcttacattc aactaaatca
     6121 ccccctttct ttttttcatt acatctttat ctttcattgc atttttcata ataaattctt
     6181 caaatgatat tctatcaatc atgtactttc ttcctatttt taaagcaata aaatcttttg
     6241 ttatcatggc ttctcttgcc atgtttctag ctgttacatc agatattttc aagtactcac
     6301 aaaattcttc tatagtcatt aactccattc ttttaattcc ttctctatct aaaaacatct
     6361 tgatgatgtc tgttgtatca tctcttcgca ttaactcttg tactaagtct tttgtgtcta
     6421 tgaattgtaa tgctacactc attttcaaac ctccttctca tattaattga atatcctgta
     6481 tttagttttc aaggtactgt catgatttaa cctaattttt gcttaaatca cttgatattc
     6541 catattttaa agccatatct tttacaatag ccacataccc ctctattagc ttcttatcat
     6601 cttgtattac atctaaattg ttaactttct ctcttttaga ttcagataca ccttcttctg
     6661 ccatttttct tcttttattg attaatcttc tatgtaggtc aactccaaat ctcttattta
     6721 ataattcata actttctgtt ctaagcatat ttatatgttc aaaaccacct tgtttttttg
     6781 ctattcttgc aattagttga tgtgtatttg ttctccaact atttgagtct aatgaaacta
     6841 catcttttat tgtttcaacc tctgtctttg cttctaaagc aatgctattt gcttgattaa
     6901 cttgaagtct taaatctttc atttctttta aactttctat taatacatct tctatacaag
     6961 ttggcttatg ttgcttaact ttgaaatatg tttcttctaa gttatcaaac tgctcccaag
     7021 ctttgtcagt atccaatatt ttgcagtgtc tatttgctcc tctttcagtc caaagataca
     7081 tttttgaagt aaattttagg ttttcatatt ctgtatgaat acctttaaaa ttttttaaat
     7141 catcaccttg caataaaaaa taatgtttac cttcaataaa tctatctttg ttattgttaa
     7201 aattgttgct tatatttctt gcatctgttt catatacatc tgctagttgc tgtgtagtta
     7261 aaactctttc gttatttctt tctattactt gtaagttatt catatttttc agctcctttt
     7321 tctttaaatg tacttttagt acagttgttt ttcaaaaaaa tatactctat tgaagtattg
     7381 aaataagctg ctatttttac agctgtattt agagacggta ctctttcgcc attctctaag
     7441 aaagctatat acctacttgt aagacctaat tcagctccta actgatttct acttagtcct
     7501 ttttgtattc taaaatcttt tagtttattc attttttcac cttcttttac tttatcttat
     7561 gtactaatag tacagtacta ttagtacatt gtcaatagct tttttaaaaa aaatgagcta
     7621 taattgataa ttaagaacta atagttcaat ataaattaaa aaggtggcta aataacatga
     7681 ttggatatag aataaaggaa ttaagaaaag aaaaagatat tactcaaaaa gaacttgcaa
     7741 cttttttagg tcttactcct aaaatgattt ctttttatga gaaggaagaa agatttcctc
     7801 cacatgacat aattttaaaa ttatcagatt tttttaatgt atctacagat tatttgcttg
     7861 gaaaggtcaa tgtaaaaaat atagacaatc ttagtgagtt agagctaatt gaaaatctaa
     7921 atttctctga tgatataaaa gaagctttaa aactaattag cgaattaagt ccttctagtc
     7981 aagaaaaaat gttcaaaata gcaaaagtat ttcttgaaga agaacttaat gagaaaaaat
     8041 aagaaagaga agaaaactat tctctttctt ttttattttc ttgtttataa tattctttta
     8101 ataattctat gtacttttct aacttctcac tattattttc tcttaatttg ttcaatgtat
     8161 cccctgtgtc aattagtagt ttttttgatt tttccacacg ccttccccct actatcagaa
     8221 cttacgttct tatttttagt caaaattccc taatgaattt taataaaata taactatgta
     8281 ttttcatttc taaaaatatt tttagattat tcaaaatttt cttggataat tatcttactt
     8341 acataataat acttttgtta aatatatgca ataaaaaaag gggaattgta acaaaaaaat
     8401 cgaattttgt agagtcttgg ttattttttc cattcctgtg tattattaat cgtcttttta
     8461 attataattt tttgtatttt
//
LOCUS       DACXMZ010000175         8471 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.176, whole genome shotgun sequence.
ACCESSION   DACXMZ010000175 DACXMZ010000000
VERSION     DACXMZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8471
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.176"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            123..1004
                     /locus_tag="JD016_14875"
     CDS             123..1004
                     /locus_tag="JD016_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HAU5000914.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPGRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(1065..2273)
                     /locus_tag="JD016_14880"
     CDS             complement(1065..2273)
                     /locus_tag="JD016_14880"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAU5000915.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     RIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(2346..2598)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2750..3718)
                     /locus_tag="JD016_14885"
     CDS             complement(2750..3718)
                     /locus_tag="JD016_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HAU5000916.1"
                     /translation="MNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDFIVPVFFC
                     IICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINKPTMVMDD
                     LVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLMIALFPFG
                     MVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICVSYLAIFE
                     VLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYARILFLII
                     ILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            3891..4331
                     /locus_tag="JD016_14890"
     CDS             3891..4331
                     /locus_tag="JD016_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU5000917.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGVICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4658..4885
                     /locus_tag="JD016_14895"
     CDS             4658..4885
                     /locus_tag="JD016_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAU5000918.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            4911..7031
                     /gene="feoB"
                     /locus_tag="JD016_14900"
     CDS             4911..7031
                     /gene="feoB"
                     /locus_tag="JD016_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAU5000919.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(7164..7982)
                     /locus_tag="JD016_14905"
     CDS             complement(7164..7982)
                     /locus_tag="JD016_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAU5000920.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            8201..>8471
                     /locus_tag="JD016_14910"
     CDS             8201..>8471
                     /locus_tag="JD016_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000921.1"
                     /translation="MKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKAVKLE
                     IFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
BASE COUNT         3001 a         1211 c         1112 g         3147 t
ORIGIN      
        1 gtcatataag ttacacttta gttatcttag ttacaacttt gtcggtttac tttttctact
       61 ttcttcgata taattataat atatttttga tacaattcga caaaaggggg aatgctgtaa
      121 ttatggtaaa aaaaagaaaa aagaaaactg ttacacttac tatacttgtt gtaatagtta
      181 ttggtatttt ttctatggca tgggcggaca tgtctagaaa aaaagaaatg aaagaaactc
      241 aagaaagact taaagctgaa agattagaaa aagaaagaat tgaaaaagaa aaaaatgaac
      301 ctattattgg tgcaaaaaaa gaagctgtaa aagaatatgg atatgacgca aaaatagttt
      361 gggacaaact taacaaatac gactattcaa acaatggaga aaagatagtc tttcttacat
      421 ttgatgatgg accatctact actaatactc cacaagttct agatatcttg aaaagacatg
      481 gtgttagagg aactttcttt attaagggtg attctcttga aagaaaagga gctaatgaaa
      541 ttttaaaaag aacatttgac gaaggaaatg caatagccca tcactcttat actcatgact
      601 ataaaaaact atatcctggc agaagcctaa atctcgatac attcgtaaat gaacttaaca
      661 aaactgatga agctatgaaa aaagtcttag gaaagaattt ttcctcaaat gtagttagat
      721 gcccaggcgg gtatatgtca tggaaaaata tggaacctct aggaaactac ttaaaagaga
      781 aaaacatggc atcaattgat tggaacgctt taaatgctga tgcagaaggt aaaaagaaaa
      841 atgcacaaga attatttgaa catgcaaaga aatcttctga aggcaaagaa atggttgttc
      901 ttttaatgca tgatacttat ggaaaacaag aaacagtaaa tgcattggac caaattataa
      961 catattttaa agacaatggt tatcaattta aaattttaat ataataaaaa agcaagttta
     1021 ttgaatatac attttatact caatagactt gcttttactt tactctattt tactagctcg
     1081 atttttacaa attttttctt tcctatttga acaactaatt cactctcttt tacaatagtg
     1141 cttaaatcct ctactttttc accatttacc tttactccac cttgagaagc taatcttcta
     1201 acttcactct tactcttaac aatctcatta gaaactagta cttcgattaa gtcaaatcca
     1261 tcttccttaa cttgaattgt ttgtatatct tcaggtattt gacctttttg gaatacagat
     1321 ttaaatcttt cttcagcttc tttagcagat tcttctccat ggtataatgt tactatttct
     1381 cttgctaagt tcatctttat atctcttgga tttactgaac cctctttcaa ctgagattct
     1441 attctattaa cttcatcagg atgaacatct gtcactaaat tgtagtattt aattataagt
     1501 tcatcaggaa tttccataga tttttggtac attattcccg cttcttcatc tattccaata
     1561 tagttaccta aactcttact cattttttct ttaccatcta atccttctat caaaggcatc
     1621 attattacta tttgagactc cataccaaat tctctttgta atgatcttcc cattagtaaa
     1681 ttgaatcttt ggtcagttcc accaagttct atatcagctt ctaatgctat agagtcatat
     1741 gcttgcatta atgggtagaa gaactcatgt actgatattg gcatttgtcc ttcatatctc
     1801 tttttaaaat cttctctttc taacattctc gcaacagtaa tagttgctgc taattttatt
     1861 acatcttcaa aatttaactt ggctaaccac tcgctattaa atcttacaat agttttttct
     1921 ttatctaaaa ctttgaaaat ttgttcttca taagtttttg catttgcaag aacttgctct
     1981 gttgttaaag cttttctagc tttagatttt ccagttgggt ctccaatttt tcctgtaaaa
     2041 tctccaatta ttattactat ttgatgacct aaatcttgta attgtttcat ctttctaaga
     2101 actactgtat gccctaaatg tatatctgga gcacttgggt ctagacccaa ttttactatc
     2161 ataggctttc cttctttttc tgatttaata agcttttccc ttaactcttt ctcatctatt
     2221 aaatcatcta cacctttcat aattattcgc atttgctcgt ctatcgattt catttttcat
     2281 tcctcctaaa atatttatat aatctataat ttttattttt ataataaaaa aagctttcat
     2341 cctcttaaaa ggacgaaagc attgcttacg tgttaccacc ttagttcatc aatatctcac
     2401 gatattaatc tcttagagta caattacata tactcgtgta ctataacgtg tactaatccg
     2461 atatctctta atggttgtta tctttcagag atagtgctct gagatgtatt caaactaaat
     2521 caatttattc catttcacca aactggaact ctctgttaaa taaaatatag ccctacttct
     2581 tctcttcata gcatttatat attcagatta tttcatcaat tacaagaaat ttttcttaac
     2641 ttactcataa tatattatta tttttatatt ttgtcaacct tttttattat tatatacatg
     2701 tttttttaca ttatactact tttttatatt ttttaccaat acccaaagtt tatttttcaa
     2761 tcaaaagatt tatttgttta ttgactatat tactctgact caatcttata tttattttct
     2821 tatttaaaaa tgcgtttata attattaaac caataactgt tacatcaaat ataaaaaaca
     2881 gcaataaaat tattatcaaa aataaaattc tagcatataa taattttcta aacttagcta
     2941 ttataaagtt ttgtagtgac atacatgagt ctctaatgat aaaatagaca cttatatagg
     3001 ttattgatat aaagattgtt tttaaataaa cagaatcaac tttaacattc aacagtttta
     3061 gaacttcaaa aatggctaga taagaaacac atatgtaaaa agcttttttt gagcaacata
     3121 aaaattggtt taaacttcta aaattcttaa atattaaaaa tttttttaaa gaattagaat
     3181 ctaaaatatg caatttattc cctaaaatta aacttaataa atatatacaa aacaccatac
     3241 caaaaggaaa caatgctatc attaaataat aaataaaatt tgtataccca ttaaaataaa
     3301 tccatttgct atatccttga aattctttca taaaactata acctattaat gttgaagcat
     3361 atatatcaat gaaaaccatt acaataactc caaatactga accatatact aaatcatcca
     3421 taaccattgt aggcttattt attagatagc cacacattat accaagtaat acactctgac
     3481 ttgcccaaat agcagttcct agatttccaa gtaccagaga aattattaaa agtgaagtaa
     3541 ttccagataa aatggtatac tttaaatcac atttcaagca gattatacaa aaaaatactg
     3601 gcactataaa atctaagtaa atagcatatc caaatccagt actcacagct ataattgtta
     3661 ctactatgaa taaagatgac aataaagctg cttcagttaa ttttttagta gtattcaaaa
     3721 cactgcctcc ataaatcata aattttctaa ctaaagtttt gtttttaata tagtatatca
     3781 ctttttaaaa atatttttaa taaatataaa ttttaataaa ggtatttatg ttaaaaatgg
     3841 tataatgtat cttaatattc agttttttaa gatgctagga ggtaattact atgtttaaaa
     3901 ttaaacactt taatgatttg agtttagatg aattttatga aatagcaaaa tcaagatatg
     3961 aggtatttgc ttgtgaacag aagatatttt cattaaacga ttatgatgat atagataaaa
     4021 gctcttatca catattttta aaagaaaatg gagtgatttg tgcttatgct agaataattc
     4081 ctaaagaata cagctcttat aatgatgttt ccattggtag agttttagtt ctttctagtc
     4141 atagaagaaa aggattagca aaacaaatga tggattgtgc tatagatttt ataaaagtta
     4201 acttacacga aaataatata accttatcag cacaaactta tataaagaac ctttacttat
     4261 cttgtggttt taaagaaatc tcagaagtat acgacgaagc aggtattgaa catattaaaa
     4321 tgagattata gtgttgacaa gctaatattg ttaatatata atttaagtat aaattaaata
     4381 ttgaattgat atttaggtat ttgaaaatac cttacataga gtcagaaggc ttttgctttc
     4441 tgactttttt attatcaaaa ttcaataatt atatggattc aaagtgttaa tttttattct
     4501 atattctact ttccccaaaa gtaagaatta tacccaactt tgtatgatag gcatcttgcc
     4561 tatctttttt ttgtaaaagt aacaaggtaa taatataatg catagtttaa aataagaaga
     4621 ttagcccagg ctaaatttca agcatggagg tgctattatg aaaaacctta acgatattca
     4681 agtgaaaaaa actgtcaaag ttgaagatat attatctagt ggtaatttaa gagaaagaat
     4741 gttagcgctt ggtcttacta gaggagctgt tatagatgta gttagaaaag gtccaaaaaa
     4801 taatttaact gtctataaaa tcagaggtag caagatagct ctacgacaag aagaaagtag
     4861 cttaatatta gtttctgaca tttaaaaatt tctagcagga ggatattaat atggggttga
     4921 cacataactc aactaaaatg agttctttaa aagacatgtt tgatatagac aataaggaag
     4981 accagtttgt aattgcattg gctggcaatc caaatacagg taaaagtact gtatttaatc
     5041 atcttacagg gcttagacag catactggaa attggccagg aaaaactgta gctacagctc
     5101 gtggtaattt taaatataaa aataccgaat atgcattaat agatttacca ggaacatatt
     5161 ctttatttgc cttatcacaa gaagaaattg tagctcgtga ttttatatgt tttggtaatc
     5221 cagatgcagt aatagtagta tgtgatgcta catgtcttga aagaaattta aatttagtat
     5281 ttcaagtgat ggaacttaca gataaagtta ttttgtgtat taatttaata gatgaagcta
     5341 gaaaaaaagg cattactatt gataaaaaac ttttagagga ttctcttggt attccagtta
     5401 ttttaactgc tgcaagaaat ggctctggta tggatgaact attggatact ttaaatgatg
     5461 tatcttttga caaatataaa ttaaataata aacctgttag atacaatgaa aatatagaga
     5521 atgtggtaaa atcaattcaa ccagaattgg ataatattat accaggtata aattctaggt
     5581 ggttgggact tagacttatt gatggcgatg aaagtatatt tgaatctatg agtaattata
     5641 tagacaaaga ttctatagat gctattaatg aagtaaagaa aaagattcca gacaatataa
     5701 ataaacagaa aataagagat gagtttacta aaatcaacta tgattatgct aaaaaactga
     5761 gtgatgagtg ttgttctaat gttgctaaaa aatcaactga tagagaagaa aaagtagata
     5821 aaatattaac atctaaaata tttgggcttc caataatgct cttattatta ggtactatat
     5881 tgtggattac tatagaagga gctaactatc catccacttt attatctaat ttattactcg
     5941 gatttgagcc aagtatttcg ggtatattaa atagtattaa ttgtccatca tggttaaatg
     6001 atatgttggt tttaggatta tacagaacat tagcatgggt aatctctgta atgctacctc
     6061 ctatggcaat attttttcca ttatttaccc tacttgagga ttttggatat ttgccaagag
     6121 ttgcatttaa cttagaccac ctatttaaaa aagcttgtgc acatggaaaa cagtgtttga
     6181 ctatgtgtat gggatttgga tgtaatgcag ctggagttat tggttgtagg ataattgact
     6241 ctccaaggga aagacttata gctatactta caaacaactt tgttccttgt aatggtcgtt
     6301 ttcctacttt gatagctata tctacaatat tttttagctc agttataacc aactctttcg
     6361 tatcaagcgt agcaactgca ctttgcataa ctttactgat aattctaggt gttataataa
     6421 cattgttggt ttcttatact ctttctaaga cgctattaaa aggagttccg tctacattta
     6481 cattagaact tcctccttat agagttcctc aaataggaag aactttatat acctctataa
     6541 tagatagaac aatatttgtt ttaggaaggg ctgtaatggt agccatacct gctggtgtca
     6601 taacttggat atttgcaaat atatatatag gagatttaag tatattatca catgttgcaa
     6661 actttttaga ccctttagct aaattaattg gtcttgatgg ttttatatta cttgctttta
     6721 ttcttggatt cccagcaaat gagatagttg tacctatact tttaatggct tacctagcaa
     6781 ctggttctat gatagaatta gatagcttta gtgctttagg tcaagttctt agagaacatg
     6841 gatggactta tttgacagct ttaaatgtta tgctgttcag tttactgcat tggccttgtg
     6901 caacaacact tttaacaata aaaaaagaaa ctggtagttt aaaatggact gccttaggat
     6961 tcttaatgcc tactatactt gcttttgttg tttgtttttt aacaactaca gtttacaatt
     7021 tatttatata attagtatta actgacagta gacatatatt agaaaaacta tttaaaaatt
     7081 tatatacttt attataaagc atagagatat attcaatgat aagtaaaatt acttaacatt
     7141 tgaatatatc tctattttaa ttcttatctg gatacttgga ttgaaatttc agtcatataa
     7201 ttatcttcat tactttcaca aaagaagtca agtatactct cctcatatga atttccaata
     7261 attttatatt cattttcata tatatatttt ttcaattttt cgtaagattt atatgaatcc
     7321 tcatattttc caatatgttt tatgcaagca tacattcctt ctggctttac atatagcttt
     7381 ttatccttta tttttttatc tatcttcaaa taaaaacttg atatacttaa atagtcccca
     7441 ttttttatat tttcattact tactattact cccaactcat atccttgata caaattttta
     7501 gatttacaat aattggtgaa atctattaat tccatcataa aatcactatt actcatatta
     7561 ctcaaatcgc ttgatagaac taaatattct tcaaactttt tggatatata aatttcttca
     7621 ctattcattt tagaaattcc ttcaactgtt atatttattc tattttgcat cttagaactt
     7681 atctttctta gtttttttat tttttctctt atcttttttt cttcttcttt aagcattttc
     7741 acacaaagct ggggagttct attttgtata tactctttta tttcctctaa actcattcct
     7801 atttctctca atgattgtaa tatatcaagt gtatcatatt gataaactga gtaatatcta
     7861 taattatttt tatctttata ttcaggagaa aaaataccta ttttgtcata gaaaattaat
     7921 gtctgcttag atataccaca caatttagca aattcacctg ttgtaaaata ctgtttttcc
     7981 aagggttata cactcctttt ctttttgttg tctatatagt tactatattc tttattattt
     8041 actaaatcaa gtagttatta ttttagaaaa tcaatatggt tttgatttta aagcttaaat
     8101 atcagttaga tgtcagttat aaatatttta accattccca tcataaaaaa gatatcccct
     8161 cttaactttt ggatggaata ttatatttat acttgttaga gtgaaaattg taataggtgc
     8221 tacaataaga tatttaatta aacataaagt taattattgt aataaaaaat ctttagataa
     8281 attagagtta aagtatggaa atatagatag agaaaaagct gttaaactag aaatatttta
     8341 tcagtattta atagaacttg aatatataga aatgagttta ttaataagaa gattagatac
     8401 tgcaataaca tcattagcat tagtatctat tataacaata gcatcatatt atttaatcag
     8461 aaaaaatgtg t
//
LOCUS       DACXMZ010000176         8353 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.177, whole genome shotgun sequence.
ACCESSION   DACXMZ010000176 DACXMZ010000000
VERSION     DACXMZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.177"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     regulatory      194..237
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            338..1306
                     /locus_tag="JD016_14915"
     CDS             338..1306
                     /locus_tag="JD016_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAU5000922.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENVLKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFISMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVIEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            1308..1871
                     /locus_tag="JD016_14920"
     CDS             1308..1871
                     /locus_tag="JD016_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAU5000923.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            1879..2568
                     /locus_tag="JD016_14925"
     CDS             1879..2568
                     /locus_tag="JD016_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAU5000924.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            2695..3423
                     /locus_tag="JD016_14930"
     CDS             2695..3423
                     /locus_tag="JD016_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAU5000925.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            3437..4111
                     /locus_tag="JD016_14935"
     CDS             3437..4111
                     /locus_tag="JD016_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAU5000926.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            4128..4973
                     /locus_tag="JD016_14940"
     CDS             4128..4973
                     /locus_tag="JD016_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000927.1"
                     /translation="MLNMNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDK
                     NEIKIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKK
                     IISSDSKALLDMTLMPGKVAYIECGHKTFVKSVEVYAYEYEAEGKIVYVNLKENTINI
                     RNNNIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNEN
                     GEYLTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHK
                     VKIDK"
     gene            4989..5660
                     /locus_tag="JD016_14945"
     CDS             4989..5660
                     /locus_tag="JD016_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5000928.1"
                     /translation="MKVLIVEDNKILLESVVEELNKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            5650..6882
                     /locus_tag="JD016_14950"
     CDS             5650..6882
                     /locus_tag="JD016_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU5000929.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKIIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            7032..7349
                     /gene="trxA"
                     /locus_tag="JD016_14955"
     CDS             7032..7349
                     /gene="trxA"
                     /locus_tag="JD016_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAU5000930.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            7357..8244
                     /locus_tag="JD016_14960"
     CDS             7357..8244
                     /locus_tag="JD016_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAU5000931.1"
                     /translation="MSIMRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLV
                     KAPSIDNYLGFYDISGDELKNKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMY
                     EATTVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEAN
                     FLSELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIE
                     VDGVFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAG
                     QGQIAALNAVYYLDKLKRA"
BASE COUNT         3457 a          782 c         1471 g         2643 t
ORIGIN      
        1 tttaaatgcg ccacatctta ttaaagttgg ttcataattc taaataaact ttaataagat
       61 acattaaaat ataataaatt cttttatgct ataaaattta ttttatagct attaaatcag
      121 atgtttaaaa attttttaac agtctatagt ttttaagagt ctatagtaag tagcctaaaa
      181 atatgatttt acatctgtac ttgttcgaga actccaagta taaaaaacta aggaattata
      241 tagtggcagt atataattaa tacccttaga cacacctttt tgaaagtgga aaaaagatgt
      301 gtagttctcc tcgaccacaa atcaaggagg ataaaaaatg gaaaagagaa aagtaataat
      361 tgattgtgac ccaggaattg atgattcttt ggcaattctt ctggctttaa actcaccaga
      421 gctagaagta attggaatta ccacatgttg tggaaatgtt ccagcaaata taggtgcaga
      481 aaatgtacta aaaacacttc aaatgtgttc ttcactaaat attccagtat atataggaga
      541 agaagcacca ctaaaaagaa aacttgtaac agctcaagat acacatggag aagatggtat
      601 tggagaaaac ttttatcaaa aggttgtggg agctaaagca aaaaatggag cagtggattt
      661 tataataaat actttacata atcatgaaaa agtatcaata atagcacttg caccacttac
      721 aaacatagct aaagcactta ttaaagataa gaaagcattt gaaaatctcg atgagtttat
      781 atctatggga ggagcattta ggattcatgg aaattgctct ccagtagcag agtttaatta
      841 ttgggtagac ccacatggag cagattatgt ttacaagaat ttatctaaaa aaatccacat
      901 ggtaggttta gatgtaacta gaaaaattgt acttactcct aatattattg agtttataaa
      961 tagacttgat aagaagatgg caaagtatat aactgaaata actagatttt atattgattt
     1021 ccattgggaa caagagggaa taattggctg tgtgataaat gaccctctag cagtagcgta
     1081 ctttatagat agaagtatat gtaaaggatt tgaatcatat gtagaagtta tagaagatgg
     1141 aatagctatg ggtcagtcta tagtggattc tttcaatttc tataaaaaaa atcctaatgc
     1201 aattgttcta aatgaagttg atgagaagaa atttatgtac atgtttttaa agaggctttt
     1261 taaaggttat gaagacatta tagactctgt ggaaggagtg atatagtgtg aaaagaaaaa
     1321 tatgtgtaaa aactattaca cttatagctt ttggtatttc tataaatata ataggcgctt
     1381 ttatagctat gggattaaga ttacctgtat acttagattc aataggaact ataatgatag
     1441 cttcactttt aggacctaaa tacgctgttg ttacaggtgt gtttggaagt ttaataagtg
     1501 gaattacgtt tgatgtgtat tctctctatt ttgcaccagt tcaaatatca accggattac
     1561 ttgctggaat agcatttaaa aagaattttt taaggggttt aaaaactcca ctaggagtac
     1621 ttttatttgc cattccaaca tctataataa gttcaataat ttcagcattt ttatttggag
     1681 gtatgacctc ttcaggttca tcatatatag tgcaaatctt gaaagtgtta ggactgggag
     1741 atgtttttag tgtgtttgta acccaagtat ttactgatta tggagataag ctgttagcag
     1801 ttgttttagt gaatttagga ttaaatgcag ttccaaaaac tttaaaggta tcattgactg
     1861 gaggaaaata gatagaaaat ggatagatat agtgagataa caaataaaaa tcaaagagaa
     1921 attgttctgc ttaaaggatt tccttgtata tggggaaagt gttctttttg tgattatata
     1981 gatgataatt ctaatttaga agaagagatg aataaattaa atctaaaggt tttaaaaaac
     2041 gtaactggga aatatggagt tcttgaagtc ataaattctg gaagctgttt tgaattacct
     2101 aaggatactt tagaaaaaat aaagtgtatt ataaaagaga agaatataaa aaaattattt
     2161 ttagaaagtc attggtctta caaaaatagg cttaaggaaa tgagagagta ttttgaaatt
     2221 cctgtggtat ttaaaattgg agttgaaacc tttgacaatg attttagaaa taatatttta
     2281 aataaaaatg caaattttaa gactccacaa gatgtaaaag aatattttga ttcaccttgt
     2341 attatggttg gaataaaagg acaaacaaaa gagatgatag ataaagatat agagattatc
     2401 ctaaatactt ttgaaaaagc gacagtgaat gtttttataa ataattcttc tagtataaaa
     2461 agagatgaag agttagtgaa atggtttgtt agtaagtata aattcttgga tgaaaatcca
     2521 aatatagaag ttttatacaa taatacagac tttggtgtag gagattagca ttaatttagt
     2581 ttaaatttaa aattgaaaag tttcttatgt ttttgaaaaa gcttctaata gttagaagct
     2641 ttttttgtag agttttatgt tacaataaga aaaaaggaat taaggagcaa ttaaatgatg
     2701 tttgataggg gaaaatggaa taaattggat tataatgaac ttttagaata tatgatgtct
     2761 atacaagata taaaatatag agattttaat aaaaagctta tacctggaac agaaaatata
     2821 ataggtatta gagttcctaa tttaagaaag ttatcaaaag aaataagcca agggaattgg
     2881 aaggaatttt tagaagtagc agaggataca tactatgaag aagttaggct acaaggtatg
     2941 gtaattggca atataaatag taattttgaa gaaactttat actatgtaaa aagatttata
     3001 cctaaaatcg ataattggtc agtttgcgat ggattttgtt ctgatttaaa gtctatgaaa
     3061 aaatataaag aaaatatgta tgatatactt aaaaaatatg tttattcaaa gaatccatgg
     3121 gaaattagat ttgcattagt tatgttttta atatattatg ttgatgataa acatattaaa
     3181 gaaatatttg aatactgtaa taatatacaa agtgaagaat attatgttaa aatgggtatg
     3241 gcatggttgc tatcaatttg ttttattaaa tgtgaagagg aaacttttct ctatataaaa
     3301 aataataatt tagatgattt cacttataat aaaatgctac aaaaaataat tgaatcaaat
     3361 agagtagatt tagaaaagaa gaatataata agaagtatga aaagaaagac aagaagatgt
     3421 tgacggagga atattaatga ataacaatga agacagaatt gacaaatttt tagaacttca
     3481 aagcaaagga tttaaatttg cagagatagc tcaaaatcta aagattagtc aaagtacttt
     3541 aagaactttt ttaaataaaa aaggatataa gtcagaaaaa ggtaaataca tattaaaaga
     3601 cgaattaaat tataataaaa gtttggataa tagaaacttg gaagaagaga aagctaagga
     3661 gtatgaaaaa agtaaaaaga atactataag taatagaaaa aaagtaagca ccaaaaaaaa
     3721 gatttctaat gaaaaggctt taagtaaaag tgttgaacaa attgagttta atgatacagg
     3781 tttaaaaaat gcaaagccaa aacaaaatgt taagcctaag aaagatagaa aaataaatat
     3841 aactcaagaa gatttagata agctttgtga agtatatgat tggtatttag aagtaaaaga
     3901 ttacaatata aagcctaaaa aagtatctaa taaaaaagat gtacatatag atgatgaaca
     3961 catagatgaa ttaaaaacaa caagaatacg tatagataaa gaaatatgga atgattttga
     4021 aagattatgt agtaattcga attttgaaaa acatcaaata cttacacagg cattgaaaga
     4081 atttatgaaa aagtataaaa atctactata aaatactact atatgaggtg cttaatatga
     4141 ataaaatagt aaaaaaatca atttctctaa tgattatatt aacaatattt atttttatgt
     4201 tgacagcatg tgaaaaagat gaacagcctg attcagttga tattcaaaca gaagataaaa
     4261 atgagataaa aatagatgag ggaaatgcaa aagtattgaa tataggaaaa tcagaaatta
     4321 taaatataga tgaaggtgat aaagtagata catcaacaaa ggtagaaaat aattcaacat
     4381 ttaatatatc caatgttgaa ttgatatata atgaatatga tgagaataaa aaaattatat
     4441 cttcagatag taaggctttg ttggacatga ctttaatgcc tgggaaagtt gcttacatag
     4501 aatgtgggca taaaactttt gtaaagagtg tagaagttta tgcatatgaa tatgaggcag
     4561 agggtaagat tgtgtatgtt aatttaaaag aaaatactat taatataaga aataataata
     4621 ttaagctaga aaatagtagt cagtatgagg tactgtctac atcagaactt aaaaaagtaa
     4681 atgaatcaaa tgagggaatt acatatcaag taaaagtaaa aaattcatca tcaaaagatt
     4741 taggaaatat tattcttaag acagcagaag ttaatgaaaa tggagaatat ttaacggtaa
     4801 atcgtgtacc atcatataag attttaaagc cttcagaaga gacagatata gatatattgt
     4861 gttcaacaaa ggcaaaaagt gttgaaatag tggggtatac gtatgatgat ataaaagaaa
     4921 aagccaatgt ggatattgac ttaaaatctc ataaagttaa gatagacaaa taaaggtagg
     4981 ggatacttat gaaggtttta atagttgaag ataataagat acttttagaa agtgtagtag
     5041 aagaacttaa caagcatttt gaaactgaaa agtgtgaaga tggagaagaa gctttatact
     5101 tagtaaatca aaatatatat gatttagtca ttttagactt aatgcttcct aatataaatg
     5161 gatttgatat cctaaagaaa atgagagtta ataatataga tacacctgta cttatattga
     5221 cagcaaaaga aacattagat gacaaagtag aagcatttac tataggagcg aatgattatt
     5281 tgacaaaacc tttttatatg gaagagttag tagctagggt atacgctata ttaaggacta
     5341 atggaaagat aaaagaaaga aacggattag aatttaaatc tttatattta gatacactag
     5401 aaaaaagagt gtatattgaa aaagaagaaa taaaacttca aaacaaacag tttaacttgc
     5461 ttgagtattt tgtattaaat aaaggttcaa ttttattaaa agagcagatt tatgatagaa
     5521 tatggggaat tgattcagat gcaactattg aaatagtgga agtatatgta agtaatctaa
     5581 gaaaaaagct aagtaaatat ggatatgata aatatattaa aactaaacgt aaggtaggat
     5641 atatattcga tgataaataa gaatgtattt accagtacta aaaatcattt aataaaaatg
     5701 tatattattg tagtaggtag ttttttaata atcttttcta tatttatata ctcatatttt
     5761 aggggactta catatagtgg aattgatagt gagataaatg atgaacttga atatatagtc
     5821 tctcaattta aaagaacttc atttttgaac ccaataagac tcaaagaccc taaagatatg
     5881 gtgtatgtat atgaagatgg aaggataagt tactatactc aaaatgaata ttttgatgag
     5941 ttacttccag atagaagact agataaaaaa aattcgtttt ttaaatacac agaaaatgga
     6001 tatactttta gagaattaaa tgtagatgta ggtagatacc aaatacagat aattagaaat
     6061 atagattctg agatgaattc tttaagacag cttacatctg ttcttataat tggaatatta
     6121 atttctgtaa taattactta ctttgtggct gtatatctca ctagaaaagc tttaattcca
     6181 atagaaacag catggaaaaa tcaagctaag tttatacaag atgcttctca tgaattaaga
     6241 acaccaataa caatagtttc atcaaaatta gaaagtatgt taaaatcccc agaaagtaca
     6301 gtaaatgatg aagttgaaac aatagctact gcaatgaagg aaactagaag acttaaaaaa
     6361 atgataactg atttattgtc tcttactaaa gaggactcta ttgtcaaagt taatttagaa
     6421 gaaatagact tagaaaagtt attggaagaa atatcagaag actatattga cattgctgag
     6481 tttcaagaaa aaagatttgt gtttaattct aaactgaaga ataaaattat aataacagat
     6541 aaaaataaat taagacagct tatattgatt ttcattgata atgcctttaa gtatacaaaa
     6601 ttgggtgatg agatagcatt agaattaaaa gaagatatag aggatgaggt aactttactt
     6661 ataagtgata ctggtatagg cataaaaaaa gaagagatac cccttatatt tgatagattt
     6721 tttagaagtg aaaatgtaag aaataaagat ttagaaggtt ctggaatagg actttcaata
     6781 gctcgcatga ttagtttaaa cttatctatt gatataaatg taacttctga tgtagatatt
     6841 gggactacat ttgaattgtc aataccaaaa aagttaaaat aataaaaata taaaaaattg
     6901 tttttagaaa tgaagaatgg gtatactaat tagtataaaa tataaaaaga ccttataaaa
     6961 gtaaatttat atttataaat ttcatagtat aattatatag ggataataaa gtttaatagg
     7021 aggatttaaa aatggcaaaa gttataaata catctgaatt tagaagtagt gttgaaggta
     7081 gtaagggagt agttgtagtt gatttctttg caacttggtg tggaccttgt aacatgttgg
     7141 gacctgtatt tgcagaatta ggtgaagaga tgaaggataa agctagattt gtaaaagttg
     7201 atatagatga gagcttagaa atagctcaac aattcaatgt atctacagta ccaactatga
     7261 ttatatttaa agatggaaaa ccagtagaaa ctttaattgg attcatgcca aagaacaaaa
     7321 ttgaaatgca agtaaaatca tatttataaa aaatgggtga gtattatgag atatgatata
     7381 gctataatcg gcagtggtcc agctggattg tctgctgcta taaatgcaaa aataagaaat
     7441 aaaacaatta tcatgtttgg taatgataat ttaagtaata agcttgttaa agccccttca
     7501 atagataatt atttaggttt ttatgatata agtggtgatg aattaaaaaa caagtttaaa
     7561 tcacatatag atagtatgga tatttcaatt gaaaataaaa gaataaataa tatttatgct
     7621 atgggagaat actttacaat aatgtctgga aatgatatgt atgaagctac tactgtaatc
     7681 cttgcaactg gggtagagta tactagacca atcaagggtg aagaagaatt tctaggaaga
     7741 ggtgtaggct actgtgctac atgtgatgca ccactttata gaaataaaaa ggtagcagtt
     7801 ataggatata atgaagaatc taaagaagaa gcaaattttt taagtgaact cacttcaaag
     7861 acttatttta tacctatgta caaaaaagat aatcttatga gaagttcaga taacctagat
     7921 gattctattg aagttataca tgacagacca gtacagatag atggagataa acttgtaaac
     7981 aaagtatctt ttaaagaaaa ccacattgaa gttgatggag tattcgttat aaaagatagt
     8041 acagctccaa gtgcattggt tcctggaata gagattgatg gcattcatat taaagttgac
     8101 aataatatga agactagtat agatggatgc tttgctgctg gagattgtgt tggaaaaccc
     8161 tattcttata taaaagcagc tggtcaaggg caaatagcag ctttaaatgc tgtttactat
     8221 ttagataaat taaagagggc ttaatattta tattgattaa ctttttaaag atatttataa
     8281 aaaactgtct tgaaataatt ttattatttc aagacagttt tttattttta aattattaat
     8341 aaaagtattt taa
//
LOCUS       DACXMZ010000177         8335 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.178, whole genome shotgun sequence.
ACCESSION   DACXMZ010000177 DACXMZ010000000
VERSION     DACXMZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.178"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     regulatory      8..93
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            217..6180
                     /locus_tag="JD016_14965"
     CDS             217..6180
                     /locus_tag="JD016_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HAU5000932.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENVSYYNIVFNNLPVGKYNVEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDESNDKQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKILTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKARMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPEAEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNAINISNKKVITVSNKVKGNIVRDK"
     gene            6390..8270
                     /locus_tag="JD016_14970"
     CDS             6390..8270
                     /locus_tag="JD016_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HAU5000933.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNHKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVVVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNI
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
BASE COUNT         3551 a          967 c         1558 g         2259 t
ORIGIN      
        1 agtagtgaaa attaaatggc aaaattaggg aaatctaatg acgcaaaact atagggatta
       61 aagcttttaa agccatatca gccagttgct aaaaagagta aagtcttttt attttataat
      121 taagattctc tttgagtgtc tttttttatt gtctgaaact tatagtattt aaattcatga
      181 aaaattagat atttattaag ggaggattgt tatttcatga aaaaggcaat atcttgtgta
      241 ctagcagtat ctatgtgtag tgcaccacta aacgtttttg cagaacctat attagagggg
      301 aagctaaggg cagttgaaaa atcagttaca gaaaagttaa gggggagtct tgaagtagat
      361 ttaaattttt cactgccaat aaaaaatagc gagtcagaaa gtatgactaa tatgcagtta
      421 cgattaaaag atgatagtaa caatagtgga ataataaagc ttggagaaaa agattcagga
      481 acagtaaatg ttgggacaga taatatagaa tatactataa aaaaattatc tgcaagtaga
      541 agtgaaataa aaaataaaga tgagaatgta tcatattata atattgtatt taataattta
      601 cctgttggaa aatacaatgt agaagtctct ggagctggat ttaagaacaa agaaattaaa
      661 gacattgata tatcaagtta ttcacaaaga gtcttattaa gcaatagagc aagtgtagat
      721 aataaaaata aacctttaaa ttatgaattg ttcttaatgg gtgatgtaaa tggagacggt
      781 aaagtagata agagtgatta taataaggtt ttagagaata tagattcaaa taaaagagaa
      841 tttgatttaa atagagatgg aaaggtagat attgttgact tagattatgt acagaaaaat
      901 ttaggacaaa gtgaagatgc aaaaagccta gaaagtatag tatctaccaa tcctatagta
      961 gatacaagta aggttgaatt aaaaggaaat agtgatgtag atattaatgg taatgtagaa
     1021 aatttattta gtggagaaaa taatggagtt acaatatctt caaaaaatga tgaaatatct
     1081 gaggaaaaac cagcagtaat ggatatagct ttatcagaag cacttaatat ggaagtcatc
     1141 aatataaaag ctccagaagg tactgctcca gcaaaaggaa tagttgaggt tactgacaag
     1201 gatggtaatg ttcaacagat tccttatgaa aacaaagaat atatgaaaaa cttaaaagat
     1261 gaggagcaac aagaggaagt aaataacgaa caagaagaag taaatcaaga agaagtaaat
     1321 aataaagatg aaaatataaa ccagactgat aataactcaa ataataaaga tgaaagtaat
     1381 gataaacaac aggaaaatag cgataaacaa gaagtaaact ctaataccaa tcaagataaa
     1441 aataatgata aagaaaattc atcaatggat aaacctaatt cagatgaaga tactccaaaa
     1501 gaagagggta atatcagtaa agatgataac tctacaacat ctacatatag taggtcagca
     1561 ttagaaacat atgcagatgt tcaaaatagt gatatagtaa taaatcttgg taaacaagta
     1621 gcagtaaaaa agataactat aaaaataaca gctacaacag caaacgatag aaatcttgca
     1681 gaaatatcaa aagttgaatt tttaaacaat gtatataaag aaataccaaa gccagagatg
     1741 aacataccta aaataaataa aatattgact agcactgctg ttggaaatga aaaagtatct
     1801 ttttattgga ataatgaagt aaatgtatca gcatacgaac ttatactaga caaaattgat
     1861 agtaagggaa aagtattatc tactaaaaaa ttacaaacta gtaaaaatac aatagaagtt
     1921 agagatatag aagcatatgg actatataga gttagcatac aatctttaaa tggagattgg
     1981 tctagtggtt ataaagatgc aacaccagaa aacttagacg gagtaccaga aaatgtagat
     2041 tcaaactata aacctataca atttagccca gatagtatat tagaatttca agttgttcca
     2101 gacacaaaac cagaaccacc agaaggtata aatgttaaag gtaaatttaa atctcttgat
     2161 gtaagctgga aaaatcataa acaagctaaa gattttgact tgtactataa agaaaaaggc
     2221 tctaatgggt cttggataaa atacaatgat aagcttattc gtgggacaag ttcaaagata
     2281 gaaggtctta aggataatac aacatatgaa gttagaatga cagctactaa ccacttggga
     2341 actagtggaa tgtctaaaac atatataggt acaactgaaa gcatggatgc accaacaaca
     2401 cctaactata gattaattaa tactcctaaa gagaatggtg gatttggaat accaacaaat
     2461 cacattgtag atgttactta tccagcagga tatgacaaaa gtgaatatag tgataaaaat
     2521 ccatttgata aattcaatgt agtagatggg gactttacaa cacattggac attcccaaca
     2581 tataatgcag ctgagggaac gaatagagga ccaataataa cttttgataa atcttatact
     2641 atggatactt ttatggctac acctcgtttt gatgtttctg ataagacaat aaatgacttt
     2701 gaggtacgat attgggatga aaatggtcaa ttacacaatc tgggggatgt tccttttgaa
     2761 acaaagacag accctgtaaa taataataag tatgtaatgg ttagattaaa agaaccaata
     2821 actgctacca agatacaagt aagtgtttgc tcatcctatt catataagtc taatatatca
     2881 gaacttaagt tttatgagta tgacagtata gaagatgatg taagaaattt atttatggat
     2941 gacctacaag ttgaattaaa agaagatgta actcaagaaa aaataactga attaaaagat
     3001 agattaaata ctccagataa agctagtgga gaatatcatc catataagac agttattgag
     3061 agagaattaa aattagctca agacttatat aatgatagag ccacccttac tgatgtaact
     3121 actgtaaatc aagaaataaa taaattagga aaatataaag ataaaaatgg acagtatact
     3181 gtaaattcca ataatttagg tatgcaaaat gactggcagg cattaggagt agctgctaga
     3241 gcaggagatg aaatcacagt atatgttgga agtaaaagtg gaaaaactcc taagttgata
     3301 tatacacagt actatggaga atctggtgca tataagagtg gagaaataaa tcttaacact
     3361 ggaaaaaatg taattcaatt aagcaaactt catagtttag atatagaatg tggaggaagt
     3421 ttatacataa gatacgctga agatactcca agtggaggag atataaaagt acgtgtagca
     3481 ggagctacta agataccaca tcttaatcta aatggaacaa taaatgataa gagtcctgcg
     3541 ggagtttctg aatctaagaa aaagataaaa gcatatatag aagaattagc taaatataaa
     3601 gatgctgtaa aaggagaacc atattatcca ggaacaaatg ttggtaattc atatggatat
     3661 tcagaaaaaa ctggtgtgtt aaacactaca gatattgaaa gtgataaagt aacattaaat
     3721 gttcctgcaa cagctgtata tcaagggata tcaaaaggaa atgctgattt aaatacacaa
     3781 gtagatagat tatataatag tatgcttgca tgggaacaaa taatggattt agtttactca
     3841 gaaagaggag tatttaaaac acaagattta gatggtaatg gaactgtaga tgataatgaa
     3901 tttaacttaa ctaaaaatga tatggctcca aaagctcgta tgaacataaa ataccaaaga
     3961 atgtttattg gagcatttat gtatgcttct ggattacatg taggagtaga atatggttca
     4021 gttccagggc ttatgaatgg agtacctttc caaatagatg atagtggaaa agctacagga
     4081 ggaaacttat ttggatgggg aataggtcat gagataggac atgttacaga tattggaaag
     4141 atgacatata gtgaaactag taacaatgta cttgctttac ttgctcagac ctttgatgat
     4201 aagacacatt caagacttga aggttcaaca atggataaga tatatgagca tgtaacttca
     4261 aatagcttag gtataccaag caatgtattt gaaaggcttg gaatgttatg gcaattacat
     4321 ctagcttatg atgatgattt tacgggttca atgttaaaga ataactcaga tgctgattta
     4381 agtaatgata cattctatgc aaagatttca agaaaataca gagcactttc aagtagtgac
     4441 ccaattaata gtttaccaaa agaccaaatg ctagtagcaa tggcatcaag tgttgttgaa
     4501 aaagatttaa gagattactt taaggcatgg ggagtagaaa ttacaccaga attaaacagt
     4561 ataatggata gtaagaacta cgaaaaagaa tcaagaaaga tacaatatgt aaatgacgaa
     4621 gctagaagaa agattttaaa tgaaaatata gctagtatgg caaaagatac taaagtaagt
     4681 gctagttttt ctgatggaat acaaagtggt tctctagtta aatctaataa aatatctata
     4741 gacctagatg tagataagga taaagataag atactaggat acgaaataat aagaagtgat
     4801 ggaaattatt tagatggtga aaatggaaca caggtaaaat acagaacagt tggatttgtg
     4861 gatgcaaaag gaaatagtaa gactaaattt actgataata tatctcctct taataacaga
     4921 gcatttacat ataaggtagt tgcatatgat tatcacctaa atccaacaga agaatttgaa
     4981 gttggtactg taaaattatc agatgaaggg aaaataaata aatcagcatg gtcattcata
     5041 acaaatactg tttctgacgg tgatgttaga actgaaaatg actcacatgg accattacaa
     5101 aatcctgaaa ttgataatat aaaagataat gatgcaagta ctacatataa aggaaagata
     5161 ataagtaaag cagagtggaa caaagaccct caaaaagacc ctgatataaa tgtagaagaa
     5221 aatccatata taatagtaga catgaaagaa acattaccta tagtaggact aaaatataca
     5281 aaaccagagg cagaagctag gaagttctca ttaaaaggat tatttaactt tagaaagaat
     5341 actagtacaa catacaatcc tttaactaat tacaagatac aagtaagtaa tgataaaaag
     5401 aactgggaag atgtaagtca aggaactttt gaatatggac aagatttagt tggtggagga
     5461 aaagaccaag ataatgaagc tagagttaca tttaataaag atggtaaact ttggacatac
     5521 caagctagat atgtaaaact aatctcaaat aataagtcta atatagaagt tgcagaatta
     5581 gatataatag gacctccagg tgataatata gaaattggca caatgaataa tgcagaccaa
     5641 actcgtacta atggaattgg aaaattagca gaagattatg tatatcaaag tgatgactct
     5701 agcacaagtg aagatgaaac aggtaagatt ccagcaggtt caatagttat tacaggtgaa
     5761 tacagtggaa atccagcatt taatttacca ctacttatag ataaaaacaa taagactata
     5821 tctggagaag cacttttatt tgcagaagtt ccagaaaaag gtgaactagg tgaaatcagt
     5881 aaaggtacat gggtttacta tatgccaaaa gaaaactttg attctttaag tgataaggtt
     5941 aaggctgaac tatatagata caatgacctt caaggagata ctccagtagg tcaaagattt
     6001 gtaagtgata caatgtatgt acctgtagga gctaagaatt atgatgagtt aaagacaata
     6061 agtcttactg atagtaatag taaagctaga aaagctacaa gaaatgctat taatatcagt
     6121 aataagaagg ttataactgt aagcaataaa gtaaagggta atattgttag agataaatag
     6181 taggtggaac tctaaaaacg taaaaatact aacactaaca ttgtatatct aataagatta
     6241 ggtactagaa tagttttaaa atagtagata ttaaaggtaa agtatataaa tgtaactata
     6301 agtttaaggt aatttataaa ttaaatataa aagctaggtg gataagtaaa tttatccact
     6361 tagttaatga tagataagga cgggtgttaa tgagtaaaag aaaatctttt ataagaacta
     6421 tagcagtatc aactatggca gtagctgtaa ctggtagtgc tacatgtgca tatgcagctc
     6481 cagttttaca aggaacaaaa acgtatgaga aagtaaatac tatagatata tctgtggact
     6541 cagtagaaaa catagtatat tcatttcaag ccagtataaa agtacaaggt gaggttgaag
     6601 tagtagataa tgagcagaaa gaaaagatta cttggtctga taatataaaa tctcagataa
     6661 aaagtggaaa tgcaaatgct acttgtagag tagaatacaa caaatcaagt aatacaacta
     6721 cattagatat atatgttaca tcaaatgaag atttactaga cggaaataga ttgaatatag
     6781 gaagaatatc tgtaaaaaaa tctggcagta actcaaatgc tgactataaa gttttaggaa
     6841 aaggaacaaa ccataaacca gctttaaaaa ttgtaacata taacaacaaa acagtggatt
     6901 atgaaaatat aagctctgat gaaggattaa tatttaccct tattaatgag agtgaagtaa
     6961 aaccaattgg tggaactggt tctagtaaaa atgaccctga aaagtataag gtagagaaaa
     7021 gtgaagcact tgaatattta ttaaacaata ttaggataaa ctattctatt gttagtaaag
     7081 aaacacaaga aagtggttca aatgtaatat taaaacttgg attagcacaa aaaacaacta
     7141 agggaagaaa agctacaata aacaaatatg ttgaagttac acttccaaaa agtttagaat
     7201 acatagttga aaatgaatta tcaaaaccag atgaattacc accagataat ggcaatggtg
     7261 gaaataatgg cggaggaagt aattcaggtg gctcatctag tggtggaagc tctggaggag
     7321 gaaattcaag tgattctaca tctaatgtca cagtcaaaaa attaaaaggt gctgatagat
     7381 ttgaaacagc tatcaaaata tctcaaagtg gttggacaaa atcagataca gttgtaatag
     7441 taaatggaga agataaaagt atggtggatg ggcttacagc tacaccactt gctagtgtaa
     7501 aaaactcacc aattctttta tcatcaaatg aaaagcttcc tcaaaaaact gtagaagaat
     7561 tgaaaaggct aaatccatca aaagtagttg ttataggtgg aaacaactca atgccaaatt
     7621 cagttgttga agctatcaaa gctgtaaatt caaaaatatc agtacaaaga ataggtggag
     7681 atacaagata tcaaacttct attaatatag caaaagaaat agataagacg aataatataa
     7741 gcaagttgta tataggagca ggaaatggag aagctgattc attatcaata gcatcactgg
     7801 ctggtaaaga aaaaactcca atagtactta cacaaaaaga tggtgtagat aatgaagctg
     7861 aacaatttat taaatcaaat aaggtgtcaa atatatattt tattggtgga gtagaaaaaa
     7921 tatctaacaa ggctattgaa caagttggaa aaatagcaaa caaagatata tcaaataata
     7981 gggtggcagg tcaaactaga caagagacga atgccaaagt aatagataaa ttctactctc
     8041 aatctaagct agatggagtt gtagttgcaa atcaagacaa acttatagat gctttagcag
     8101 ttggaccatt agcagctaaa aacaattcac cagttatact tgctacaaat acgttagata
     8161 aatcacaaga atcgtcatta aaaggtaaaa attcatcaaa gttgtttgaa gtaggtggag
     8221 gaatagcctc atctgtaata gataagatta agagtttaat tgaaaaataa atattaagta
     8281 aatagaaaat aagaatgtat ttagatataa agttatatat ttaaatgcat tctta
//
LOCUS       DACXMZ010000178         8327 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.179, whole genome shotgun sequence.
ACCESSION   DACXMZ010000178 DACXMZ010000000
VERSION     DACXMZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.179"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            319..1254
                     /locus_tag="JD016_14975"
     CDS             319..1254
                     /locus_tag="JD016_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAU5000934.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(1346..3217)
                     /locus_tag="JD016_14980"
     CDS             complement(1346..3217)
                     /locus_tag="JD016_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAU5000935.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYVSLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            3407..4333
                     /locus_tag="JD016_14985"
     CDS             3407..4333
                     /locus_tag="JD016_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAU5000936.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            complement(4723..5247)
                     /locus_tag="JD016_14990"
     CDS             complement(4723..5247)
                     /locus_tag="JD016_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU5000937.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(5300..5524)
                     /locus_tag="JD016_14995"
     CDS             complement(5300..5524)
                     /locus_tag="JD016_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000938.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            5721..6845
                     /locus_tag="JD016_15000"
     CDS             5721..6845
                     /locus_tag="JD016_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HAU5000939.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(6957..7061)
                     /locus_tag="JD016_15005"
     CDS             complement(6957..7061)
                     /locus_tag="JD016_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000940.1"
                     /translation="MWKFYVLKILVKTFIKKDNRNVLTKIVRIEMLYR"
     gene            complement(7319..8143)
                     /locus_tag="JD016_15010"
     CDS             complement(7319..8143)
                     /locus_tag="JD016_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000941.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
BASE COUNT         2825 a         1301 c          892 g         3309 t
ORIGIN      
        1 acttatatac tttatgtgta aattatggta aaaatattat ttaatcaaat caaaacttga
       61 tttatatacc tggaaaaggt attatattaa agagtgctta aaatacgcat ttcataaata
      121 gctatatata aaataataaa taaggagagt gatgggcaat ggatactagt ccgttgcgga
      181 gagaattttt tacattttca tcacttaata atatctaaaa tcaaaactaa tacatatatt
      241 cagttatata aattagttaa ttaatagata ctattaaatg atgagattta tataaaactg
      301 aataaaggat gtgttgccat gataagatat tacaaaacta tagattcaaa attagagaaa
      361 cttagttttt ttgaagatgg ctgttggata aatcttgttg aacctaatca tagtgaaatc
      421 aacgaaattt ctaatttatt aaatatagat gtagaaagca tagaatctgc actagatgaa
      481 gaagaacgct caagaataga tgttgaagac aatcatactc ttatacttat agatatacca
      541 gtagacgaaa gtgactctaa ttcttctcat tatactacaa tacccttagg tattatacta
      601 actgaagaag ctattgtaac agtttgtgat gcacaaacta agattttaaa tgactttata
      661 gttggtcata ttaaagattt ttttacattt aaaaagactc gctttttgct tcaaattctt
      721 cacaaaaatg cagcttatta cttgcattac ttaagaaaaa taaataaaat gacaataata
      781 atagagcgag aaatttacaa atcaatgaaa aacaaagaat tggttcaatt attggaatta
      841 gaaaaatctt tggtatactt ctctacctca ctaaaatcta atgaacttgt tttaaacaaa
      901 atggttagaa ctgctggtat aaagaagtat ccagatgatg aagacctatt agaagatgtt
      961 atagttgaaa atagacaggc tcttgatatg gctaaaatat atggagatat attgagtaga
     1021 attatggatg ctttcagtgc aattataagt aataatcaga ataatgttat gcaaatttta
     1081 acagtagtta cactaatttt ctcaatacct acaataatat caggattctt tggtatgaat
     1141 gtcatcaaca tgccattttc taataaccca aacggatttt ggataatact tcttattacc
     1201 tctataatat gtatagtgat tacatttttc atgtcaagaa ataagctatt ataaaatatt
     1261 aataaaaaag ccattagaat taattgattt acatttatcc taatggcttt tatatattta
     1321 aatctatata atcttataat actttttaac tatcatgttc ttcatctata atcatttcag
     1381 tttcaagccc ttctagcatt gaatctaaac tttcttttat atttagtata taaattggta
     1441 catactcttc ttcatctatt acctcttctt gcaacttaaa tggaggaatt aatacttcat
     1501 caaagtctat atttaaatta ggaaagcctt ctactatctc ttcacaatta tcgtactctt
     1561 catttgctaa agaaacgtac atatctaggc tctttttata ataaagacct gcaagcatat
     1621 actcacactt cttacttcca tatttaatac cttctttata atatttctca gcttctgcgt
     1681 catcatttaa ttttaaataa atttgaccta aattatatat tgattcaaca caaccttgtg
     1741 ctctagcctt ttcataccaa aatttagaat ttacataatc tttatagaac atatcataaa
     1801 ttaatcctac catatgttga gcatacacat tattttcatt ggcaggtgta tcaaacatca
     1861 ttttagactc ttccaattta ccttctctaa aataaattct acctagatgt atacgagcat
     1921 tcatatggtt tttgctactt gccatttcaa aatactttct agcatttttt gtatttccta
     1981 agctctcata tatacacccc aatctataac tagacctcgt atctccattt tcagatgctt
     2041 tcttatacca acttattgct tcttcaatat tatttaaatc ttcatagata tttcctaatt
     2101 tgatttgaca agatacatta tttggaatcc tcgaataata cgaaatcgct ttttctatat
     2161 cttgattctg ataatataaa tcccctagat tttcaaggga acttttgcat ccaacagcta
     2221 tagcatcctc ataatattta atagcatttt cataatcttt ttcttcaaaa tatatacctg
     2281 ccagattatt ttttgcctct gttatatcat cttcagcagc cattttataa taagtttttg
     2341 ccttctctat ctcattttct ctattatata aattagcaag tctatactta gaaagtgtat
     2401 gatttttttc tattgctttc tcataataat aaattgcatc tcccagctct tcttttctat
     2461 catatacata tgcaagccta tactcagact tcacatcatc tctatcagca ctctttttat
     2521 aatacttctc tgcatcttct aaattatcat ttatttccat aatcataccc aagttaaagc
     2581 aacctttcat atgacctgac ttgtataact gtttatataa acttactgtt gatttattat
     2641 ctttcttttt tccatttaat attgctaaat tatattttgc atcatcatct aaaagcacag
     2701 aacctttttt atagtattca tatgcttcat catctttctc caaaaaagta tataataccc
     2761 ctaaattaaa acaagcttta cctaaattct tgtctgctgc tgtttttaac catttttcac
     2821 accttacaaa atctttcatt ttaaaatggc atacacctaa attattttgt gcttgtaaat
     2881 aacctaaatt tgctatcttt tcataccaat atatagcata ttcatagcca tctaacttat
     2941 agtacatatt acctaaatca aaagcagcat cttcacttcc acttttatat gccattgaat
     3001 accacttttc tgcttcttta aattgttttt tactttcata tatatttcct aaagtatatt
     3061 gagattttac gtaattatcc aatgcagcac tcttatacaa ctctattgcg ctttccatct
     3121 tacccatctt ttcaaggagg gcacccttag tatgtacgac tttcgggtca ttagatatat
     3181 tttttatttt ttttatagaa aattttgtat ttttcattat tcgctaccta cccattttat
     3241 aaaatatatt ctctcaaaca ttattataca ttataatcta caattaatgc tataatattg
     3301 aatttttacc ttttgtcact atattgtaac attaaatttt aaattttata atcattagat
     3361 tttattatgt aattggtata taatgtatat atagggggga cattttatga atttcagcaa
     3421 tatttttatc caagttgcag ttttatttat aattatttta gttggatatt ttgtcagaaa
     3481 attcaactta ttagatgacc actgcacatc taagctttca actttgacaa tgacagtttt
     3541 tttaccatca atgataatta gctccatgca aattaacttt gatagtaaaa tgatacaaaa
     3601 aatactattg ttattattta catctttgat tatgtatata gtttcaatta taattgcatt
     3661 tttattgaaa tacattttaa aatgtaatga taaaaaagat ttgggaatat atcagtatat
     3721 agttgttttt tcaaatgttg cttttatggg ttatcctgta attgaagcag ttttaggtca
     3781 tgaagcaata ttctatactg caatattcaa tttgcctttt aacttatttt ctttcacttt
     3841 aggaatttat cttttatcta aaggcagtac aagtagaggt ttttcaataa aatcacttat
     3901 aagccctgct acaatagcta ttgttattgg acttttttta tttgtaacag gacttaggtt
     3961 accccaattt ataaacgagc cacttgagat gttgggaagt ataactacac caatatccat
     4021 gattatcatt ggaagcttac ttgctaattc ttcagctctc gattgttttg tcaacaaaaa
     4081 attatatata gtcactttta taagattatt ggtacttcct gtgattgtgt attttattct
     4141 aaaaggatgg ataaatgata agatgatttt agctatacca gtagtaattt cttcaatgcc
     4201 tgctgctgca aatactgcta taatggctaa tcaatatgac tccaatataa cactagcttc
     4261 acaatgtgtg ttttttacaa cactattttc agttatatct atacctttta taagtatttt
     4321 actcttatct tgatttattt ttttatgtat tatataataa catattcaat aaattacaaa
     4381 tataaacatt ttattgtagt atattgatgg aattttttcc tagatatttg tcaataatat
     4441 ctatatgtat tatagaatag gaggcttaaa tatggatatg gatttactct ttaaaatagt
     4501 atttgctata gttgctatag gtgtttctgt aaaaatcatt aaaactgttg ctagtttagc
     4561 ttttaaaata gcgttgatat tgctaataat tttatttgta tatagaatgt ttttgtaaac
     4621 atttaaaaat aagtttttta acacaataga gaagtaaaat agattatctt taatgtaaat
     4681 tttttattaa agacactcta ttttacttct ctattagctt ctttaaatta aatttttatt
     4741 atatcttccc ataatataaa catcttcaaa ttttccatct ctgattacag catatttttt
     4801 agttccctct atttcaaaac ctagcttttt atataaattt aatccctttt cattatccac
     4861 aattacttct aatcctactc tagttaacat aatccaatta tctgctatat ctaaaagttg
     4921 ttccataagc ttcttaccta ctcctcttcc ctgataattc tctgcaacac ttattcctat
     4981 ttctgcgcta tgtctcaatc gtggagaaga atttacatgt aacccagcaa aacctattac
     5041 atctttttct ccattattat tagtttcagc tacaaacata taatcatttt ctcccaaaga
     5101 agctaacata gcttcagttt tatcgactct gtcactcatt aatgccaaag tatttctcat
     5161 aacactgtat gttcttctaa tttcattaaa agcttgtgca tcttctcttc ttataggtct
     5221 tattgttata tctataggct tactcatatt ggattccccc tcaaacataa tctttatttt
     5281 taattaaaat tttacatctt tattttatac tgttcaaatc tatgtatgct tctacctgag
     5341 gtacatatat catattatct tttatatgtg tacttccaaa ttcttttaat tctgtacctt
     5401 ctttatcctt agttatttta tatctaatgg attccatacc ttcaactact tcagcatgaa
     5461 aaaacatacc ttcatattgt ttaaatctaa aattaaaatt gtctttagta agttttatta
     5521 tcatattttt atctactcct tatataattg atatttttct tattattagt tgtatatata
     5581 ctgcttattc atattaatat attataactt atcatttatt tagtataaag ttataaatag
     5641 atatatttat acttatttta caatctttct tcaattgata tataatatgt ttatataaac
     5701 taatgtatgt gaggtgaaat atgagcaaaa aaacaaaata tttttcttta attctctttc
     5761 ctattgcttt gcttttaaat tttatagcca gtaaagttcc aaatattgtt gaaaaatatt
     5821 actcccaatt catagataaa ataattgtac aaatattgag taaagtttct ggaattttcc
     5881 cattttctct ctatgagatt acaatgtaca taatagttat atctatcttc ttatttttat
     5941 gttgtacttt atctacaatt tttaataaga aaaaaaaatt aaacatatat ttaaaaaatt
     6001 ccattttaaa tatattatcc attgtatcaa tagtctactt tctttttgta gtgctttggg
     6061 gaataaacta taataggatt ccactagaaa ccacacttat aaataactat aatttaaaaa
     6121 acaatacctc tatccaatca aaacaatata gtgttaaaga gcttactaaa ttatataaat
     6181 tccttgtaat taaagcaaat gaaactagaa aactcacatt acaagataaa aatggtattg
     6241 taaaatctaa tacagattat aatggtgtca taaatagagc tcaactagga tacgataata
     6301 tcttggatat attacctggt gttagtggta gttattctaa gcctaagtat attatttcat
     6361 caaatctaat gtgctataca ggaataactg gtatatactt tccttttaca ggtgaggcaa
     6421 atgtaaatat agcaatacct gacttatata ttccttgtac agttgaacat gaaatggcac
     6481 atcaaagggg atttgctagt gaagatgagg ctaattttat agcgtatctt acttctataa
     6541 aacaccccaa tattgatttt aactattctg gatatatttt agctttaaat tatactgctt
     6601 ctgctttaag taaggttgat tataatgcat atgttgatat ttctgctggc atttcagatt
     6661 ctgtccgtag agatttaaaa aatgaaagtg aattttggca aaaatatgaa ggtaaaatta
     6721 atgaaatttc taatgagttt aacaattctt acttaaaagc taatggcgtt actgaaggta
     6781 ctcagagtta tggtaaaatg gttgatttat tgctaactta ttatgaatta tatccatata
     6841 actaaacact acaattcaaa attttattca tatacagctg atattaaatc agctgttttc
     6901 tatttaaata aattatttaa cttcaagcaa tcttatataa tttgatagta cttatactat
     6961 ctgtataaca tttcaattct aactatttta gttaaaacat ttctattatc ttttttaata
     7021 aatgttttaa ctaaaatttt taaaacataa aacttccaca ttatttcact atctatataa
     7081 aaatctaaaa attcacatat aaaattacat aactaaaaaa tcataaattt atagaaaaca
     7141 tatattcaaa ataaaaaagc atgtatcaaa acttgacaca tgcttataat cttatgtata
     7201 tatatcattt tgtcacaata aaaatttact tgtaacactg atatttttaa acaaaatagt
     7261 ccttatttaa ttaaattaac attaattaaa ttcgtactca ttttgctatc attaaacatt
     7321 acttatagta attttctaat acactatttg aaacttcctt cccttgaaaa cttttcaacc
     7381 aattctcata tgctgagttt ttccatacac taccatcatt aaatgttact tgttttacac
     7441 aagatataat gtactttaca ccatgctctc cagttgattg tttccattta tcaaacaata
     7501 gaaaggcatc attttgattc ttacctggtg ctattgcatt ttgctttgca tattctttct
     7561 ccatttgttt ccattctttc aaccaatttt ctacccatgc cttgccttgt tgtttttcaa
     7621 acatacttat tatatcctgt ggtggtgatt gttgcatttt tctataagta tgtgaatatg
     7681 attttggaga cacaggagat atgcctgtaa caataccata gtctacacca gctgggctaa
     7741 atccaacact tcctacttcg ccatctgctg ccacattttg agcatcccaa tatagctcaa
     7801 gtggttttcc atttttatca tatgctaagc atgtaatttc ataatccata attgcctttt
     7861 cactgctatt tgttaagctt ataacattgc taggccaatg atatggagta tcatcatact
     7921 gtattttgct attatttata aataatggaa tattctcatt ataaacaaga cctttactac
     7981 tttgaatatc tgataccttc tttgtgacag tacttaccgt atttgctaag tatacatttg
     8041 ttttttctga attggtcgta ctaagagaaa atgttgttgt agttccagta atcagcaaag
     8101 caacaactaa aataggtaag ctagatattt ttttatatct cacagttctt cacctctttt
     8161 tctattaact tataataaaa caatttacca aaacattaaa atctatgtta acataattta
     8221 ccaactgttc tagtaactat atcctatcat agttactatc aagtttcaat actattgatg
     8281 gaaattttca ctatattaaa tcttttttta attttataaa tttacaa
//
LOCUS       DACXMZ010000179         8243 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.180, whole genome shotgun sequence.
ACCESSION   DACXMZ010000179 DACXMZ010000000
VERSION     DACXMZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.180"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            24..179
                     /locus_tag="JD016_15015"
     CDS             24..179
                     /locus_tag="JD016_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000942.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            309..1322
                     /gene="aroF"
                     /locus_tag="JD016_15020"
     CDS             309..1322
                     /gene="aroF"
                     /locus_tag="JD016_15020"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAU5000943.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1344..2408
                     /locus_tag="JD016_15025"
     CDS             1344..2408
                     /locus_tag="JD016_15025"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAU5000944.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2408..3721
                     /gene="aroA"
                     /locus_tag="JD016_15030"
     CDS             2408..3721
                     /gene="aroA"
                     /locus_tag="JD016_15030"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAU5000945.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGKTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3699..4778
                     /gene="aroC"
                     /locus_tag="JD016_15035"
     CDS             3699..4778
                     /gene="aroC"
                     /locus_tag="JD016_15035"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAU5000946.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4781..5977
                     /gene="pheA"
                     /locus_tag="JD016_15040"
     CDS             4781..5977
                     /gene="pheA"
                     /locus_tag="JD016_15040"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAU5000947.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6024..6833
                     /gene="aroE"
                     /locus_tag="JD016_15045"
     CDS             6024..6833
                     /gene="aroE"
                     /locus_tag="JD016_15045"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAU5000948.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6845..7378
                     /locus_tag="JD016_15050"
     CDS             6845..7378
                     /locus_tag="JD016_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAU5000949.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7399..8238
                     /locus_tag="JD016_15055"
     CDS             7399..8238
                     /locus_tag="JD016_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAU5000950.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         3381 a          827 c         1496 g         2539 t
ORIGIN      
        1 ttataaaaat ggaggataat actatgaaag gaatattaaa agaactaaca gcaaaacttt
       61 atagcttttt ttactttatg ttctggggct tttattttag cgctggctat ttcttttaca
      121 aaaatatatg tacttccttt ctggtgaggc cttattttag tgattttgag cataagtaaa
      181 ttttataaaa atgattattt tatatattta tataagattt agtgcttgat agattatttt
      241 agagaactca ttagtgagtt ctctttttta ttttaaatat aaaattataa tattgggagg
      301 tagtaaaaat gatagtagta ttaaaaatgg gagcagacaa aaatgaagtt aaaaaattaa
      361 ttgaggctat tgggagagaa ggggtagaag ttaatcccat agatggaaca gaattaactg
      421 ttttaggttt agttggagat actagtaaaa tagatgccaa aagaatagaa gcaaataagg
      481 tagttgaaaa agttatgcat gtggtagagc cttttaaaaa ggccaataga aaatttcatc
      541 cagaaccttc aatcataaat gttaatggaa tggaaattgg ttcaaagaaa attgctatga
      601 tagctggacc ttgttctgtt gaaacagaag accaaatagt atctatagct aaagatgtga
      661 aaaaatcagg tgcaggattt ttgagaggtg gagcattcaa accaagaaca tcaccatacg
      721 ctttccaagg attgaaatat gatggattgg atttattaaa aaaagcaaaa gaaaaaacag
      781 ggcttcctat agttacagaa ataatgtcaa cacaagatat agatatattt gaagaaaatg
      841 tagatgtaat tcaagttgga gctagaaaca tgcaaaactt tgatttatta aaagaattag
      901 gtaaaacaaa taaaacaata cttttaaaaa gaggattatc tgctacaatt gaagagtggc
      961 taatgtcagc agagtatata atggctggag gaaatgaaaa tgtagttctt tgtgaaagag
     1021 gtataagaac ttttgaaaca tatactagaa atactttgga tttaagtgca attttagctg
     1081 tgaagaaact tagtcattta ccagttatag ttgacccaag tcatgcagca ggtaaatcat
     1141 ggatggttga ctctttatct aaggcagcaa ttgcagttgg agcagatggt ctaataatag
     1201 aagttcataa tgacccagca catgcacttt gtgatggaaa acaatcgatt aagccaaatg
     1261 aatatgatga attgataagt gaattaaaga ctatcgcatc agcagttggt agagaaatat
     1321 aatctataaa aggatggtta aatgtggaag aaaaactatt agtaaattta aaagaaaata
     1381 gctacaatat atttattaaa aaaaatatac taaagaatat aggagaagaa ataaaaaaaa
     1441 tatattttgg aaataagatt tttattgtaa ctgatgaaaa tatagcaaaa ttatacttgg
     1501 ataaagtaaa aaataattta agtgaatctg gatttgaaat taaatcttta atattaaaat
     1561 ctggtgaaga aacaaaatca tttaatactc ttcctaaaat atacgatagt ttacttgact
     1621 ttaagttaac tagaaaagat ttgattatta ctttaggagg aggggtaata ggtgatttag
     1681 gtggatttgc agcagcaact tttttaagag gtataagttt tattcaaatt ccaacatctt
     1741 tattagcaca agttgatagt tctgtaggtg gaaaagttgg agttgacctt gagagaggca
     1801 aaaatttagt gggaagcttt taccaaccaa aggctgtgtt tatcgatcca aatgtattat
     1861 tatcacttcc agaaaaattc tatagagatg gaatggcaga agttataaaa tatggatgta
     1921 taaaagacaa aacttttttc aataaacttt taagtttaaa aaatagagaa gaagtaatgc
     1981 aacatataga atacattata cataaatgtt gctttattaa aaaaactgta gttgaagctg
     2041 atgaaaaaga ctttggagat agaatgcttc taaactttgg ccatacatta ggtcatgcta
     2101 ttgagaaata ctacaatttt acgggttata cccatggaga agctgttgcc ataggaatgt
     2161 ataagataac tttagaaagt gaaaagatag gaattacaaa aaaaggtaca tcagaactta
     2221 taaaatcaat tttaatgaat tataatttgc cttatgagat tgaattaaga gataataaat
     2281 ctatagtaga tgctatatct cttgataaaa aaaatctagg tgatacatta aaaataatac
     2341 ttttaaaaga tattggagag agtattatat ataatactac ttctaaattt tttcaggagg
     2401 taagataatg ggaagtctaa aaatatatcc aagtaaatta tctggagatg taaaaatacc
     2461 accatcaaaa agtatggcac atagggcagt tatttgttca tctctaagca atggaaaaag
     2521 tagaatttct aatatagatt tttctgatga tataatagct actataagag caatgacatc
     2581 attaggagct attatagaaa agaaagaaga tattttagaa atttcgggaa ttttttctaa
     2641 agaaggtatt ttaaatagag agaatcagtt gaatcaacct aaattgacaa tagattgtaa
     2701 tgaatcaggc tctacattga gatttttagt acctatatct ttagcatttg atggtgtaaa
     2761 aaggtttata ggtagaggaa atcttggaaa aagaccactt gatacttatt atgagatatt
     2821 tgacagacag aatataaaat atagttacaa agaaaatcag ttagatttga ttatatcagg
     2881 taagcttaaa ccagatgaat ttagagtaaa aggaaatata agttctcagt ttatcacagg
     2941 tctactgttt atattgccta cgctagaaag tgattcaaag ataataatta ccactgagct
     3001 agaatctaaa ggatatttag atttaactct ttcaacgata aaagattttg gggtagaaat
     3061 tataaacaat aactataaag aatttattat aaaaggaaat caaacgtata aagcgagaga
     3121 ttataaagta gaaggagatt attctcaagg agcattttat ttatcagcag atgctatagg
     3181 agaagatatt agcatattag accttaaaga agattctctt caaggggata gtgaagtagt
     3241 tgaaatttta tctaggatgg gaatggaaat attaagagaa ggcaacaaaa taaaaggaat
     3301 aacaaatggt ttaaatggta ctttgataga tgcatctcaa tgcccagata taatacctgt
     3361 attatctgta gtagcatcct taagtatagg gaagactaca ataattaatg ctggtaggct
     3421 tagaattaaa gaatgtgata gacttcatgc aataaatgtt gagttaagta aacttggagc
     3481 aaatatagag gagaaagagg atagtttgat aatcgaagga gtaagcaagt taaatggtgg
     3541 agtagaggtg tggagtcaca aagaccatag aattgctatg acacttgcaa ttgcttcttg
     3601 tagatgtgat aaaccaataa tattaaaaga ttttgagtgt gtatcaaaat cttatccaca
     3661 tttttttaaa gattttaaga tgttaggagg tcgaattgat gagtggaata tggggaaata
     3721 atttaaaagt atcaattttt ggagaatctc atggaaatgc tataggtata aatatagatg
     3781 gtcttccttc ggggattgaa cttgaccttg ataatattga taaagaaatg aagagaagag
     3841 cacctggaaa aaattctata tctacttcaa gaaatgaaag tgatatacca gaaattctaa
     3901 gtggatattt taatggaaga actacaggaa cgccattatg tgcaatcata agaaatagtg
     3961 atactcgttc aaaagattat ggagagctta aaaatttaat gagaccagga catgcagatt
     4021 tcacaggtaa tgtgagatat tctggattta atgactatag agggggaggt catttttcag
     4081 gtaggataac agctccatta gtattttgtg gggctatatg taaacaaatt ttatcacaaa
     4141 aaggaataga gataggagca cacataaaaa aaattaaaaa tatagaagat atgagttttg
     4201 attatgtaaa tatttcaaaa caacagttat caaatttgca aactttggaa ctacctttac
     4261 ttgatttatc aaaggaagaa gccatgaaaa atacgattat agatgcaaaa aatcaaggag
     4321 attcagttgg tggtataatt gagtgtgcag tagtaggtat taatgtaggg cttggaaatc
     4381 cattttttga ctctgttgaa tctactttat cacatttatt gttttctgtt cctgccgtaa
     4441 aaggtgtaga gtttggttta ggttttgaac ttgcagatat gtatggctct caaagtaatg
     4501 atgaaatgta ttatgaagga aatcaagtta agagcaaaac aaataacaat ggaggaatta
     4561 taggagggat tactactgga atgccaataa ttttcaaagt ggcaattaag ccaactccat
     4621 caattagcag acaacaaaac acagttaata taaaagataa aaaggacgat attttatata
     4681 ttaaaggcag acatgaccct tgtatagttc aaagggctat acctgtaata gaagcagtaa
     4741 cagccattgg aatttttgat ttgatgaaag gacgataata atggaggatt taacgagata
     4801 tagaaatgag attgacaata tagataaaga gcttattcaa ttatttgaaa agagaatgaa
     4861 tacagttctt gaaattgcca gacataaaat gaaaaataac actactatac ttcaaaaaga
     4921 tagagaagaa aaagtactaa gtagagccgt agataattta gataataaag catattcaca
     4981 agaaacggta cagtttttta attctataat ggaaattagt agaggattac aaaaaagatt
     5041 gatagataat gatggtgagc agaaaatcga gtttagttta gattcatcaa ataagaaatt
     5101 tgatttgtca aatgtaaata aatataagtc tttaaaagat gaattaaata aaaaaaatat
     5161 cctagtaggg tttccaggga aatcaggttc atttacagaa gaagcactaa ataaattctt
     5221 caacaaaaaa atatcaaaaa aacaatttaa agaatttgaa gatgttttca tagcacttaa
     5281 aaataaagaa ataaactatg gaataatccc catagaaaat tcatctacag gagctatctc
     5341 tgaaacttat gaccttttga gaaagtacgg tttttatata gttggagaag aatgtataaa
     5401 aatagaccaa aatttaatag ggataaaaga cacaaagctt gaagatataa aagaaatata
     5461 ttctcatcca caaggtatag gacaatgtag tgaattttta aaacaaaata gtacatggaa
     5521 attgatacca tttaataaca cagcaacaag tgcagaactt gtaagaaaac ttcaagataa
     5581 aacaaaagca gcaattgcaa gcaaaaaagc tgcaagtatt tatggattag aaataataag
     5641 tccttgtata aatgatatag caaacaacta tacaagattt gtagtaatca gtaatcaaat
     5701 acacattgaa gaagaatcaa acaaaatgag tgtagtattt tctgttgaac atgaagcagg
     5761 aaaactttat aaagtacttg gatactttgc taaaaataat ataaatatga caaaaataga
     5821 atcaagacct atgaaaaatg cctcatggag atatttcttt tatatagatt ttgagtgttc
     5881 tatatacaat gctaaagtgt acgatttatt agaattgata gaacataata cagcatattt
     5941 taaatttatg ggagtatata aaaataaaac tgaatagata agataaattt ttatttaatt
     6001 tataattatt aggaggtgta agtatgaatt ttttcggtct agtaggtgaa aaactctctc
     6061 acagtgtttc acctcaaatc cacaagagag tattcgagat attgaatatt gaaagtgctt
     6121 ataaaaactt tgaaatttca aaagaggata tatctaagct agacggtgct ataaaattat
     6181 taggaataca aggagtaaat gttactgtac catataagga gcgtattatg aaatatcttg
     6241 attttatatc accagaggca aaacgtatag gagcagttaa tacaatttta cttagagaaa
     6301 atatgttata tggttacaat acagattact ttggtcttga tagtatgttt aaaatggcta
     6361 atattgatgt tcaaggaaaa gtagcagtta tattaggaac tggaggtgct tctaaagctg
     6421 ctcttacata ttttattgat tctggtatag aaaagcttta tgtttcaact agaaaaaagg
     6481 atgataaaaa attattaaat agtaaagcta tattaataga ttatgaagaa cttaaacata
     6541 taaaaggaga tataatatta aatgctacac cagtaggtat gtaccctaat gtaggaattt
     6601 caccagtgtc aaaatctatt atacaaaatt ttgatatatt aatagattta atttacaatc
     6661 ctggagaaac agagttttta agaattggta atagtatggg taaaaaaact tgtgatggat
     6721 tatatatgct ggtagggcaa gcaataaaat ctcaggaaat ttggcaagat actaaaatag
     6781 ataatagtat tttagatgtt atatataatg aactaaaatt agaatttttg taagggagat
     6841 tgttatgata aataaaacaa aagaaaaatt gatattaata ggtatgccag gtagtggaaa
     6901 aactacaatt ggtaaacttt tggccaaaga atataattgt agtttctgtg atatggatga
     6961 ttatataatt caaatatctc aaaaaagtat agcagagttg ttttcagaag gagaagatat
     7021 ctttaggaac tatgagactc aagcatgtag agaactttct ataagtgata aaacagtgat
     7081 atctacaggt ggaggtgtaa taaaaaagga tgttaatatg gaaatactaa aagaaacagg
     7141 tataataatt tttattgata gacctataca aaagattttg gaagatataa atattaattc
     7201 aagaccatta ttaaaaaacg gaaaagatag attgtataat ctttacaatg agagaataaa
     7261 tctttataaa aaattttcag atatagagat tttaaatgat aaatcattaa ataatgctgt
     7321 atataatatt acaaatgcag tcagtgaaaa ttttaaattt gactttaaag aaaaataatc
     7381 tagggggaaa taaaaataat gaatattgtt attgtaggac taggagttat aggaggctct
     7441 tttgctaaag cattaaaaaa agctggatat gaaaatgtat ttggagtaga tgtagattta
     7501 gaaactttaa aaaaagcaga aaaagcaaaa atcataaaaa aaggatgtac tacaggtaaa
     7561 gagttattta aaaaagctga tttaattata ttatctatat atcctagatt agttgtagat
     7621 tttctaaata ataataaaaa cttttttaaa aaagggacta taattactga tactacagga
     7681 ataaaagaaa cacttatcaa tgatgtgttg caaattattc cagatgatat agattttata
     7741 tttggacatc caatggcagg tagggaaaaa aagggtattg attttgcaag tgaacaagtt
     7801 tttaatggcg caaattatat aattactcca actggcagaa acaacataaa aaatttagag
     7861 ttagttgaaa atttaatttt agagattgga tttaaaagag taaaaaagtt aacttcacaa
     7921 aaacatgatg agattatagc ttttacttct cagttacctc atgttatggc agttgctctt
     7981 ataaatagtg atgaagaagg tagagatacg ggtaaattta ttggagatag ttatagagat
     8041 ttaactagaa ttgcaaatat gaatgaagac ttatggagtg aacttttttt aggaaataga
     8101 gataatttac taaaagttat agaaaacttt gaatcagaag taaatttagt aaaagaagct
     8161 atatttaaca atgataaaaa taaattaata gagtatttta aaaaatcatc aataagaaga
     8221 gaagctttag aaaaataaat att
//
LOCUS       DACXMZ010000180         8216 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.181, whole genome shotgun sequence.
ACCESSION   DACXMZ010000180 DACXMZ010000000
VERSION     DACXMZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.181"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(124..648)
                     /locus_tag="JD016_15060"
     CDS             complement(124..648)
                     /locus_tag="JD016_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAU5000951.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(713..1465)
                     /locus_tag="JD016_15065"
     CDS             complement(713..1465)
                     /locus_tag="JD016_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HAU5000952.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1496..2794)
                     /locus_tag="JD016_15070"
     CDS             complement(1496..2794)
                     /locus_tag="JD016_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HAU5000953.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2811..5075)
                     /locus_tag="JD016_15075"
     CDS             complement(2811..5075)
                     /locus_tag="JD016_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HAU5000954.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5330..6151)
                     /locus_tag="JD016_15080"
     CDS             complement(5330..6151)
                     /locus_tag="JD016_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAU5000955.1"
                     /translation="MMFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKST
                     LFMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIA
                     FAMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIV
                     LLDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILID
                     EGKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6141..6839)
                     /gene="cbiQ"
                     /locus_tag="JD016_15085"
     CDS             complement(6141..6839)
                     /gene="cbiQ"
                     /locus_tag="JD016_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HAU5000956.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6842..7126)
                     /locus_tag="JD016_15090"
     CDS             complement(6842..7126)
                     /locus_tag="JD016_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HAU5000957.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7116..7868)
                     /locus_tag="JD016_15095"
     CDS             complement(7116..7868)
                     /locus_tag="JD016_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HAU5000958.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8050..>8216)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         2567 a         1501 c          896 g         3252 t
ORIGIN      
        1 aaataaatta aaaagtgaaa tgtatttgtc taaaaacata ataacatttc actttatcct
       61 catttttaag taatatatat ttttaatttt tatttataac taaatctagt ttttatataa
      121 attttagttt ttttctacat ctgagtaatc tattccaaaa gtttctacag taacagattt
      181 cataatttgt ggagtttgtg gcttatcagc tgaatctgtt ctaactgatg ctatttcatc
      241 aactgtatcc ataccttcta tcactctacc aaaacctgca tactgaccat ctaagtgtgg
      301 tgagttttta tgcattatga aaaattgaga accagctgaa ttaggtgcca tagttctagc
      361 cattgatatg actcctcttt catgttttaa attgttagta aatccattac cactaaattc
      421 accttttatt gagtgacctg gaccacccat tcctgtacca tttgggcatc ctccttgtat
      481 cataaatcct ggtattactc tatgaaatat tattccattg taatatcctt catttactaa
      541 tgatacaaag tttttaactg tatttggagc tatatttgga tataactcaa ttttgatttc
      601 ttttccattt tccatttcta tagttactat aggattttta ttttccataa tttattacct
      661 tccttttcat tagttaaatt acataattac atttattata ttatactcta aattaaagtc
      721 tatcaagtat attttttcct atttcatatc cttcttgata aatagtttta caaaactcat
      781 catcaaagtt aagcgtgtct ttaggagcaa attgagtatt aggtctaact actattattt
      841 tatcttcatc atatactttt ttaatatctt ctggcaactc ataattatat tttgtggata
      901 ttaaaataat tttatccaca ttatccccaa ttaatggttc aattaatgca ctcctaatta
      961 aacctccatc taatttgaaa ccatcataaa ttccttcttg cacatctttc acaaattgtt
     1021 ctttaaatcc aagagttgcg tttggatttg atggtaacaa cccactgtat gaaatgctga
     1081 taatggcttc attacgaggc atttttgata catcaacaat cttttctgat actactttat
     1141 tatcaacttc aacataatta acataaaagt tcatttcttc tatattacta tcatctagtt
     1201 cccttaggtt gtctatagca ggagaattat ccacagtatt atttactatt tttacatttc
     1261 cattctggat atcttttata tttgtccaca ttttctccaa gttatcaaca ttccctgtgt
     1321 acaaataata tccatttatt gctcctattg atgtccctga aatcgaataa aatttattta
     1381 ttcctccatc atacaaggct ttaactactc ctgcttgata agcccctttt gctcctccac
     1441 cttctaaaca taatcctaat ttcatagtct catctccaaa ttgtttatct tatatttata
     1501 tttcaaaaaa cttactaaat gttttatata tactatagtg gtcctcaaca tttcctaatt
     1561 ctcttataga atgcattgct aaaattggac ttcccacatc cacagatggt atatctatat
     1621 gagtagaaga tattggacct atagtacttc ctcctctttc atctgaccta ttaacaaatt
     1681 cttgatactc aacattagct tctctacata tatttttata aatagctata gaatatccat
     1741 cactagtata tgcctgacta gcatttatct ttataaccgg accttttccc attattggtt
     1801 tattagttgg gtcatgcttt tctggaatgt ttggatgaat agcatgagct aaatctgatg
     1861 atatcataaa agatgaataa atagactcaa agaatccctc tctatcctta cccattgata
     1921 tgcaaattct ctccaatata tttaacaaca tgtttgaatc tgcaccttgt ttagtagagc
     1981 taccaacttc ttcattgtca aatacagcca ttacatttac accatgttga cctttagtat
     2041 taacaagagc attcaaacta gcatgtacca ttgcaagatt atcttgtctc ccaatagata
     2101 taaattcatc attagcccct attaaacttc ctttttcaaa ttcatataaa aataaatcaa
     2161 aatctataat atcgtccata tttacatcta agttagatgc tatttgcttt ataagaaaat
     2221 tatctttttc taatgtatca tttaacatcc caactaaagg taacatatct ttttgcttat
     2281 tatatgagtg accatcattt atacttctat ttaaatgtat tgctagattt ggtattatac
     2341 aaattggctt tttaaagtct ataagccttt catttggttt taatatacta tcacctttta
     2401 aaactactct accagctatc ccaagaggtc tatccaacca tgtacttaat attgctcctc
     2461 catagcattc tgtatttaac ttcaaataag atttttcagc ttctatttct gcatttggtt
     2521 taatactaaa tgtaggagaa tctgaatgag agcctattat tctaaatcca tctctttcta
     2581 tatcttctga atttactaca aaagctacta tagctgataa attcttagta acataatact
     2641 ttccaccaac tcttagattc catctttgat tcaaaactaa ttcttcaaat ccatttttat
     2701 ctaagatttc cttagtactc tcgactgcat gaaaagacga cggactatca tatatgaagt
     2761 ctattaagtt tttagcaaag tttatactat ccattcctca tttacctcca ttataaaata
     2821 tttagtgttg tatattcttt atttattttt ttaattccct gttggtcaaa agctatagta
     2881 acatctgtac caattataga aaccactgtc ccaaccccaa atttaggatg atgtacctta
     2941 gaaccaagtt ttatatcatc tggattagtt tctttattag aatcttttat tgtagcatta
     3001 actttatctg caactttaga tttgcttata gtactcatat actttttagt atatttatct
     3061 aacatattat aacttgcttt agaatagctt agttcttttt ttactttgta caatctttct
     3121 atacattctt ctggcagttc ttccataaat cttgaagcta ttgcaacatt tgttttacca
     3181 tataatgttc tcttctctgt caatgacata tatagctctt ctttagctct agttatacca
     3241 acataacaaa gtcttctctc ttcttctatt tgtgaatcac tcatagattt tatagctctt
     3301 gatataggaa ataacccttc ttccatacct gttaaaaaca caacagggaa ctctaaacct
     3361 ttggatgtat gaattgtcat aagcgaaacc ctatcattgt cttcttcatc atttgattca
     3421 gatgtaagtg ctatacttgt taggaatgtc tctaagtctt tattttcata agtatcatca
     3481 ttactacttt caaactccaa agctatagaa ataaattctc tcaaattatc aattctgtct
     3541 tgagcttctt cacctttgcc tgttaaatct ttttcatttt tatttcttat ctctacaagc
     3601 tcgtccatat atcctgtagt atccaagact ttttctataa gtttgctaac aggataaact
     3661 tcttttatag ttctaagagt ccctataata tcaacaaatc cacttatact tgcccttgcc
     3721 ttagtagata tatctgaatt tgtttctata tcaattaata ctgagtatat actttcctgt
     3781 tttaagttag ctctatcttc aattttttct atagttctaa gccctatacc tcttctagga
     3841 acatttataa tcctttttat agatatatca tcttgtggat tttgaataac tcttaaatac
     3901 gccaacaaat cctttatttc ttttctctca tagaattttg taccaccata tatattgtat
     3961 ggaatttgac ttctatttaa agcgtcctct acaggtcttg cctgtgcatt tgctctataa
     4021 agtactgcaa aatccttata aggtctattt tcttttctag ctatttgtgc aattgaatca
     4081 gcaatgaaat cagcctcttc tatttcgcta ccagttaatt gtatctttat aagttctcct
     4141 tctttttttt cactccaaag cttttttctt tttctttcta tattattaga tatgacttta
     4201 ttagctgcat ctaatatcac ttgtgtagac ctataatttt gttctaactt tacaacatgt
     4261 acattgtcat aatctttctc aaattcaagt atgtttctta tatctgcacc tctccatcca
     4321 taaatacttt ggtcatcatc tccaacaaca caaatatttt gatgttctct tgctaataat
     4381 tttatcaatt catattgagc tttacttgta tcttggtact catctaccat tatgtatcta
     4441 aatctacttc tgtaatatgc taacacttca tcattagcct tgaaaagttc tacagtttta
     4501 agtattaaat catcaaagtc aagtgcacta tttcttttta aacggtcttg atatagcgca
     4561 taaatatctg caatcttaac cattctatta tctgccatat tgatatcttt aaactgcttt
     4621 ggagtataaa gtttatcttt tgctcctgaa attgcactta taactgcttt tggttcaaac
     4681 actttatcac ttaaatttaa ttcttttaga cagtctttaa ccaatgtaac ttggtctgct
     4741 gaatcataaa ttacaaaact tctattgtaa ccaatcttat ttatatcttt tcttagtata
     4801 cgaacacagc aagaatggaa tgtacttatc cacatatcct tagtttcagg acctatattt
     4861 tgttcaactc tctctctcat ttcatttgca gctttgtttg taaatgttat agctaatata
     4921 tttggtgctt gtacaccttt atcttctata agatatgcta ttcttgttgt taatacctta
     4981 gttttacctg aaccagcacc agccagtata agtactggac cttcagtctt ttctactgct
     5041 tctctttgag caggatttaa tgtatctaaa ttcatatttc tcctcctgtt aacagccata
     5101 aatatatttt aatctaaaat atgaattact tcaaattata attatgtatt atattaatag
     5161 attatttcca atatattaca attaaataac atttttccat ataatttatt tcattgttta
     5221 ataaaaaaaa taaccttatg atttggggaa tcataaggca cctttaaggg gtctaggtta
     5281 agggttatta tatataaatc tattttttta aaataggttt agggcttaat tatttatttg
     5341 tattaagttc tctttccttc caatacttat acaaatcttc ttctttctta aataagggta
     5401 aattcatatt tctatgtact tttactaacc atggggattc taggccagct tgtataatat
     5461 tattttcatt tataaacaca ttttctgctt ttccttcatc aatcaaaatc cccttatcca
     5521 atacatatat gtaatcacat atttcataca ttaaattcat atcatgactg gatattacta
     5581 tttttatatt atttttattt aatcccttta ttatatctac aatagaccta gtacttactg
     5641 ggtctaaacc agctgtaggt tcatcaagta atacaatttc attttccatt gctataactg
     5701 atgctattgc cactcttttt ttctgtccat agctaagaaa atgtactggc ctgtctataa
     5761 aatctattcc attaacagct actagagctt tgtttattct ctctttaatt gttttttcat
     5821 ccataccaat gtttctcatt gcaaaggcta tatcatcata tacttttgaa taaaaaattt
     5881 gtttttctgg gtcttggaac acaataccta cattttttct aaggttgtat aaacttctct
     5941 tatcatattt taacttttct tctttaaaaa gaatttctcc agatgtaggc tttaatatac
     6001 ccattaaatt cataaataat gttgattttc ctgaaccatt tgaacctatt atacctataa
     6061 catttccctt tgaaaaatcc atattaatgt ttaatagtgc attggtattt ttttcatatt
     6121 gatacgttaa attatttatt ttaaacatca ttatccccta ctatgtgaaa tttaccatca
     6181 tacaatttta tgtctaatga tatacacata tcatcatacc ttgtcataag tcttttgtac
     6241 agcatacttc caagaatacc taaggattta tatgaagttt ttaaatttat atatccaaat
     6301 cttagctctt gtgacttgta tatttctgaa aattcttcta aaaatatgaa tataaaccta
     6361 tatattaaca ttgataactc gacaaaagtg tcaggtaaat gaagtttttt aaaaaagaat
     6421 attaattgat taaaaggtgt tgtaagtata caaaaataca cacaagtcaa acaagacatc
     6481 gctctaaata acacatgaat agacatgtca atagacgcct ttgatattcc tacattaaaa
     6541 ctaaaaaatt caaaactata taataaatct gctttattaa aagatatatt tacaagagtt
     6601 attcctatac taagaaataa aaagtacatt ggtattctaa gtaattttaa ataatttttt
     6661 aaatctattc cagccacaca aacaaccaaa atactcatta aaatcataat tccacttaaa
     6721 ataaatatat tttgtattac catagaagca atcagaaaaa ttactcctat ggctactttt
     6781 ttgtttggat ttactttact caatctattt gtatatgcat atttatcaat tataagcatg
     6841 attaacttct ctttcctttt tgaagaccta atatgtatcc tattacacca gcccctaaag
     6901 ctgcttgtgc agagaataat aaactttcta tttctccact tggtggctca tatggactgc
     6961 taaaccatgg tttgtaattt ggatttatct tagtaatttc actttcagct tgaccatctg
     7021 ctcctccata ttcagcacct gagttgacaa gtaatgggaa tataataaga gctactacta
     7081 ataaaagtaa taatatgtta gtttttgttt ttgttttagc actcattagc tttaactcct
     7141 ttatcattat tttctagtat gcttttttct ccgttttctg taattaaatt atatacaatt
     7201 acagtaagta taccttctgc tattgctatt ggtatttgag tcataaagaa aattcctaaa
     7261 aatttaattg ctgaagccat taccccacct gatgggtctg ggaaagccag tgctaattgt
     7321 attgaagtta ttgtatatgt ggctaagtct cctattgctg ctgcaagaaa tactggaata
     7381 gcattatttc catctttttt ctttaaagcc ttaaatatta ggaatgatac gattggccct
     7441 attatcgcca ttgaaaaagc attagctcct aaagtagtta ttcctccatg tgctaataac
     7501 aatgcttgga atattaaaac tatagttcca agaacaaaca ttacgcttgg tccaaacatt
     7561 attgctccaa gacctacacc agtaggatgt gaacaacttc ctgttactga aggtattttt
     7621 agtgctgata atacaaacac aaatgctcca cataaagcta ataatacttt ctttttaggg
     7681 tcttgcttta ctattttacc tattgatttt aatccgaata agaaaaatgg tataaatata
     7741 actccccatg ctatagacca ttttactggt aagtaacctt ccattatatg cattgcattt
     7801 gatgtaactg gagttaaaat gattaacata agtgcagcta taacccctaa tttaatattc
     7861 tgtttcatct tagtatccct ctctcccttt ctaataatta aaacataata atacatgtct
     7921 gatttattta ataaaataaa aaagcctaac tcgatggtta ggcttctaat tttccacaat
     7981 ctaaatacaa acacataaaa caaacatatc tgtataatta ttataagaaa ctttctccat
     8041 ccccgaagaa attactttaa tattttaggc aggtctcctg acttagcttc atcctactcc
     8101 ttttccttcc catgagatat aaaaaatatc atcatagtgg ctaacaaaag tttcgtctgc
     8161 atcacagtag cgggggctgt aaaggaattt aacctttttc cctattaatc ttgctt
//
LOCUS       DACXMZ010000181         8158 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.182, whole genome shotgun sequence.
ACCESSION   DACXMZ010000181 DACXMZ010000000
VERSION     DACXMZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.182"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            372..989
                     /gene="nth"
                     /locus_tag="JD016_15100"
     CDS             372..989
                     /gene="nth"
                     /locus_tag="JD016_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAU5000959.1"
                     /translation="MLDELEKLYPDAKCELNYGTAFELLIATILSAQCTDVRVNKVTS
                     ELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLCELYDGEVPDSLEK
                     LIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQKTEFALMEAIP
                     KERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK"
     gene            1183..1653
                     /gene="trmL"
                     /locus_tag="JD016_15105"
     CDS             1183..1653
                     /gene="trmL"
                     /locus_tag="JD016_15105"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAU5000960.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            1689..2537
                     /locus_tag="JD016_15110"
     CDS             1689..2537
                     /locus_tag="JD016_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAU5000961.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            2910..3914
                     /locus_tag="JD016_15115"
     CDS             2910..3914
                     /locus_tag="JD016_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAU5000962.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLKIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            4040..5152
                     /locus_tag="JD016_15120"
     CDS             4040..5152
                     /locus_tag="JD016_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAU5000963.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPVNVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            5642..6403
                     /locus_tag="JD016_15125"
     CDS             5642..6403
                     /locus_tag="JD016_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000964.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            6406..7224
                     /locus_tag="JD016_15130"
     CDS             6406..7224
                     /locus_tag="JD016_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000965.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            7404..8006
                     /locus_tag="JD016_15135"
     CDS             7404..8006
                     /locus_tag="JD016_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HAU5000966.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT         3411 a          752 c         1494 g         2501 t
ORIGIN      
        1 atataattga gaaggaagag ataaataaaa aagaaatgga taagaaagat ataattgaga
       61 aggaagagat aaataaaaaa ggaatgaata agaaagatat aattgagaag gaagagataa
      121 atgaaaaagg aatagataaa atgattggta aaaaagatga gataattgat gagaaagata
      181 tagagaattt tatggatatt gaagaagata aaaaaagtat caaagtaagt aaaaaaagta
      241 ctaaaaaagg tgaaacggag aaaaaaacaa agaagagtag ttctaataag gctacacaaa
      301 aaacaagtaa agaaactaaa agtaagtcta aaattaaaaa tgagtcagca aatgaaaaag
      361 acgtaaataa gattttagac gagttagaaa aattgtatcc agatgctaaa tgtgagttaa
      421 attatgggac agcctttgaa cttttaatag caactatttt atcagcacaa tgtactgatg
      481 taagagtgaa taaagttact tcagagttgt ttaaaaagta caacacagct agagactttg
      541 ctaatttgag cattgaagaa attagtaaag agataaagag ttgtggtctt tacaaaagca
      601 agtctcaaaa aataaaggat acatctgaac agctatgtga attatatgat ggtgaagtac
      661 ctgatagctt agaaaagctt ataaaattac ctggtgttgg aagaaaaaca gcaggggttg
      721 tacttagtaa cgcttttaat catccagcaa tagctgtaga tactcatgta tttagagtta
      781 gcaatagaat aggaatagtg gacgagccaa atcctcaaaa aacagaattt gcacttatgg
      841 aagctatacc aaaagaaaga tggtcacact cacatcatgt attaattttt catggaagaa
      901 gaatgtgtaa agctagaaat ccagaatgtg caagttgccc aataaaagaa gattgtaatt
      961 attataaaga acttaatgaa acgaaataac ttttagaaag taagttgtac aagagggatt
     1021 ttctgatata ataatacttg tgaaaatgtt agataaaaaa ctattatata catgaaataa
     1081 aaattattat taacattatt ttatataata gtataatatt attgttattc tgtttataaa
     1141 ataaaatttg ataaataaaa gcgatactta gggaggatta aaatgtcatt aaatatagta
     1201 ttagtagaac cagaaatacc tcaaaataca ggaaatataa taagaacctg tgcagcaaca
     1261 ggttcaactt tacatttagt aaaaccactg ggtttttcat tggatgataa acacctaaaa
     1321 agatgtgggc ttgactattg ggatatagct gatatacaat attacgatag ctttgaagaa
     1381 ttacaagaaa agtatccaaa tagtaaatac tttttttcta caacaaaggc taaacagtca
     1441 cattcagatg tgaaatatga agaaaattgt tttatagtat ttgggaaaga aacaaaggga
     1501 ttaccagaac ctttgttgaa agcaaatatg gatacttgta ttagagtgcc tatgttggat
     1561 gttgaaaaag ctaggtcatt aaatttatca aattcagttg ctattgtagt gtacgaggct
     1621 ttaagacaaa taggataccc aaatatgagg tagtttcaac aagcctaaat gagaaaggag
     1681 catacttaat gaaaaatcta aagataatct gtgacagttt atcggacgtg ccaaaagatt
     1741 tgttagataa atatgatata gatatagttt cattaacagt tatactggag gaaagagaat
     1801 acaaggatag aattgatata tctggagatg aattttataa aaggcttaga gaagaaaatg
     1861 tatatccaaa aacttctcaa gctacttatg cccaatttaa ggaaattttt gagaaatata
     1921 caaatgagga tagagagata ctttatatag cagggtctgc cacagctaca ggtacgtatc
     1981 aaagtgctgt aatggcaaag aatgatgtag atggtaaaat atatacatat gactctaact
     2041 tgctttgtat gggaactggt ttacttgtaa taaaagcagg agaacttgca agtgaaggaa
     2101 agtctgttgc tgaaatattg cctgttttgg atgaattaaa agagaaggct tttgttatat
     2161 tttcagtaga tacattggaa tatttacaaa agggaggtag aatatcatct actaaggcag
     2221 caataggaag tatattaaat ataaaaccga ttcttgaaat aagagatgga ttagtttcgc
     2281 aaatatctca agtaagagga aagaagaatg taatttctaa gatgatggaa ttgataaaat
     2341 caaattgtgg tgatgattta tcagaccaaa ttgtgtatat aggatatagt gatgatttta
     2401 aggaaaagga acaattatct aaggttatgc aagatgaatt aagacctaaa gaaattagat
     2461 ttttccaaat tggtgtgtgt ataggtgcac atgctggtcc tggtgttact ggtatcatat
     2521 gcttaaaaaa taaataatat atttttgggt gacattattt atttttttat taaattagga
     2581 tagaatatta attattgaga tgttaaattg gatttatttt ggatgaaatt aagtgttgat
     2641 atattagatt tagtttgtag ggaagtgtgt aaatagataa gaatctattg cacacttttt
     2701 tatagtaaga tttattaaaa aaatttcaaa atgtacttgt aaattgtata taaaaaaatt
     2761 gtaaatagga tgtaaaagta aataggatgt aaaagtaaat aggatgtaaa agtaaatagg
     2821 atgtaaaagt aaataggatg taaaagtaag taggatgtaa aaataaatag gatataaaaa
     2881 tatcgtaaat gtatgaggag tgaaatttaa tgcgtttcaa aagaatattg atattgatgt
     2941 taactgtagc aatgatagct ggaatgttgg ttgggtgtgc taataaccca agcaataatc
     3001 ctagctctag caaagataat aaaagtgata taagcgataa aagtaattca aaaaaatctg
     3061 tatcaatgat actagatatt gaaggaacaa ataatgaagc tatgaataat tcagctttat
     3121 tagcattaaa caatgctcaa aagaaactta agatagatac aaacaaagtg gaatcagatg
     3181 attcctcaac tttttctaat agtatagata ttttatgtaa tgacaattac gatttaatta
     3241 tagctgtagg agctagattt gcaaaaccat tggagatggt agccaagaaa tatccaaagc
     3301 aacaatttgc aattatagac tatgagtatg acaagcaacc ttctaatata acttcaattt
     3361 cttatgaaga taataaatct ggatatctag ctggattaat tgctggaaaa atgactgaaa
     3421 gcgataaggt tggttttata ggaggaacaa agagttctag tagagataaa tttgaatcag
     3481 gttttagaga gggtgttaaa ttttctaata gtagtataaa ggatatttca gttgagtatg
     3541 cagatgtttt taaagatagc aaatcagtag aatctatagc taaaaaaatg atggataatg
     3601 gagtggatat tattttttct actactgaag atgattcaaa agctgtaata gatgcagtaa
     3661 gggcaaaaaa taaaaaagtt atagcaacga ataaagacca acatgagtta gctccagaaa
     3721 atgttataag ttctattgta aaagattttg agaatcctac ttataatctg atacaaagct
     3781 ttgtaaaagg aaattataaa ggtggaaaag ttatagaaaa cacagttaag tctggaagta
     3841 ctgggcttgc ccaaaatagt tctcaaaata ttcctccaga tgtgttagaa tatgttaata
     3901 agaacaataa ataatctact ataatatcca agaatactta tgactttagt tatgaggtga
     3961 attggatgtt ataatagaca tatatagaaa gacatatgca gaggtaggtt agacctatct
     4021 aaatcaggga gtgaattttg tgaaaataaa aaggatattg atggcttgct ttgtagtagt
     4081 ttttacaatt tttatattag ctatatgtaa ttttgatttt gggtttgata aagtaaataa
     4141 gaataattct aatatagata gtgctaaatc gaaacagagt gagtctaaaa gtttgccaac
     4201 ttcagcagaa aaggataaat caaatagtaa aagtaagaat aaagaagatg acacagaaga
     4261 tgattcatat gtaagaaaaa ctattggagt attgttagat accgaaaact taaataacga
     4321 atcatataat aacaatgtac tatcagcctt aaaaagggct gatgaagatt ttgatgttag
     4381 gataaaactt gaaaaaccaa agacttctaa aagttttaaa gagagtatag aaaaactgca
     4441 taagtatgaa ccagatttga taatagcagt tggagccaga tttgcagagc caattaaaga
     4501 agctgctact aaatatccca aacagcaatt tgcaatagtt gactatactt ataaagttca
     4561 gccagttaat gtaatttctg tatcatttca agataatgtt actggatatt tagcaggact
     4621 tattgcaggt aaaatgactg atactgggaa agtcggtttt ataggaggag ttgagggttc
     4681 tagtagagat aagtatgaat ctggatttag aaaggggctt aaaacttcaa ataaagatgc
     4741 tgagttacta gttaagtatt caaatatatt tgaaaaccct gaatcagcta gaactattgc
     4801 aaaggaaatg agagattctg atagagatgt tgtattttca gctacagaag atgatagtaa
     4861 aagagctatt caggtagcaa gagaaaatgg tggtaaagtt atagctatta ataaagacca
     4921 atacgatatg gctccagata atgtaataag ttcaattata aaagatgtag aacaacctgt
     4981 ctatgaactg ataaagaatg ttgcaaaaga tggttttaag ggaagtaaag taatggaatt
     5041 taaaataaaa gatggagaac ttggcttgac ttcaaagagc agtagaaata taccaccaga
     5101 tgttttggaa tacataaaaa aagagtataa caaagtgaaa gatacatatt aattattgat
     5161 gtagaagatt ataaaaatat actgcttaag tttagattag caataaaaag atatatttat
     5221 taaatttgtg ctaactgtac aaaatatatt gattaagttt agattagtga taaaaagata
     5281 tatttattaa atttgtgtta acagtatgga atatactgct taagtttaga ttggtgataa
     5341 aaggatatat ttattaaatt tagtctaact gtacaaaata tattgattga gtttaggtta
     5401 gcaataaaaa gatatattta ttaaatttgt gctaactgta cggaatatac tgcttaagtt
     5461 tagattagtg ataaaaagat atatttatta aatttgaacc aactgtacaa agctatacta
     5521 attaagctta gaataactat ataaaattat aacaataagt gtgtgggcag aaaaattctg
     5581 ctacacactt tatttatatt cacataaaaa atttatcaaa aaaaagaaaa aggagtaaaa
     5641 aatggaattt acaagagaac catttatact agatgacgat ttgtgtctag ctttggtgga
     5701 caaaagaatt agttatctaa tggaaaatga attaaaatta agaaatatag agataataaa
     5761 aacaatagaa tgtaaagaat tatatgaaag cataaaatat cacccggata tatgtatttg
     5821 taatttagga aaaggagata taattgttgc gccgaatgta tataatcaat acaaagagtt
     5881 attaagtaga tataaattta atataattaa gggagaagct aatttattaa atagataccc
     5941 ttatgatata ccttacaata ttgcgatagt tggtgactat gctatacaca attttaagta
     6001 tactgacaaa gcaatattag attatattga aaataataaa cttaaaaaga taaatataga
     6061 gcagggctat tctaaatgct caatctgtat agtagataga acgtctataa taacatcaga
     6121 taaaggtatt tggaaaagta tgaagaatac agaaatagag tgtttattga tagaaaaagg
     6181 tcacataaat ttatttgaga tgaattatgg atttataggt ggatgtacag ggttgatttc
     6241 taaggataaa ctagcttttt gtggagatgt taaaaaacat cctgactatg aacgtataaa
     6301 aagttttgta gaaagtaaaa acaaggagat agttacactt agctgtgaaa atttactaga
     6361 tataggttct atagttcctc tcatgacaag aaaggagcga taactttggt tgagaaggat
     6421 aatatttctt ccatgaaatt tttgaggaat aataatgtaa acgatagatt aatatatacg
     6481 aatgacattg tagaagtagt gatagattct gcaaagcaag aagtgttaaa taaaaaaata
     6541 cttaatggta ctaaagctga acaaagagct ttaaatattg atgagatatc aacggaaata
     6601 actagtatga taataagtat aatgaaagag aaacttttga aagaatacat tctaaaatgc
     6661 tatgggcgta catatcctga agataaaaat aatatataca tatattcact gaacatattt
     6721 gctaaaaaag agatttttat gagagaaaca gtatttacta gggtgagaaa ttatatagaa
     6781 gaaaatgatt ttataaacgt agaaggtttt attagattta gaatgagaga atttatgaaa
     6841 tatatttctg ctataggtga tattgcagtt gaggagtatt taataaagaa agaccaagat
     6901 gaatttataa gagttttaaa atactttata gatactcaag aagaaaagat tgatttgctt
     6961 aaagttcata taatggaaga taatacattt gttttatacg ataaaaatgg aaataagata
     7021 gacagtatag atgatgaaga aattataaat atggtaataa gagaaaattt aaattatgag
     7081 gactttttaa taagtacctt attaactcta tgtcccaaaa aaatagaaat attggatttg
     7141 ttaaataata attgctcaaa ggaaattata gatactgtag aagcaatttt tgaaaataaa
     7201 gttagtataa ttatggaaaa ctagaatcta agcatataaa agctatccat ataaaattaa
     7261 aaaaattctt tttaaatcga cacaaaagac ttataattgt atataaagct atagtatgac
     7321 tagagttatt aagtataaag aatatctact tgattaatac aatatagctg gcaaatatac
     7381 aattgataag gagtaatttt ataatggata atttaattag gatactgata ttagctgtta
     7441 ttggtggatt tataggttat gttacgaatg tagttgctat aagactgata tttagaccaa
     7501 tagagcctat taaaatacct attttaaata tagaaatagt aggtcttata ccaaagagaa
     7561 gagcagaaat agcagcaaat gtaggagaaa taattcaaga agaatttctt tctatggacg
     7621 aaatacttgc aaatatcatt actgacgaag ataaggaaga agttgtaaga tacataaaag
     7681 caagggtaaa aattataata catgagaaag tttcatttat cccgagtggt ataaaaaaca
     7741 tgatacaaga ttatctagga gagataattg agtctgaagt taagcaaagt atagatgaat
     7801 tgagtaaaaa tataataaat aaagcaaatg aacgtataga tatacaaaaa atggtcgaag
     7861 ataagattaa tgaactagat ttatatgaat tggaagagat aataataaga atagctaaga
     7921 aagaattaaa acatatagag tttttggggc ttgtcttagg gtttttaata ggtatagctc
     7981 aagggttaat tactatgttt atttaaatat atgagaagta gcatttattt aagtactaat
     8041 tatatttacc tatttgagtg aattacttat taataacatt tagatattaa ttgtatttac
     8101 ctatttgagt aaattactta ttaatagcat ttagatatta attgtattta cctatttg
//
LOCUS       DACXMZ010000182         8072 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.183, whole genome shotgun sequence.
ACCESSION   DACXMZ010000182 DACXMZ010000000
VERSION     DACXMZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.183"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..633
                     /locus_tag="JD016_15140"
     CDS             <1..633
                     /locus_tag="JD016_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAU5000967.1"
                     /translation="GTGKSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRV
                     FQDPSLGTCPSMTVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDV
                     QVKFLSGGQRQSLSLIMSCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITT
                     LMVTHNLKHALHYGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            764..1786
                     /locus_tag="JD016_15145"
     CDS             764..1786
                     /locus_tag="JD016_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAU5000968.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKDKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(1950..2507)
                     /locus_tag="JD016_15150"
     CDS             complement(1950..2507)
                     /locus_tag="JD016_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAU5000969.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTIAVGNPCRVIKKIEE"
     gene            complement(2513..3589)
                     /locus_tag="JD016_15155"
     CDS             complement(2513..3589)
                     /locus_tag="JD016_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAU5000970.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3590..4273)
                     /gene="modB"
                     /locus_tag="JD016_15160"
     CDS             complement(3590..4273)
                     /gene="modB"
                     /locus_tag="JD016_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAU5000971.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4422..5234)
                     /gene="modA"
                     /locus_tag="JD016_15165"
     CDS             complement(4422..5234)
                     /gene="modA"
                     /locus_tag="JD016_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAU5000972.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5427..6356
                     /locus_tag="JD016_15170"
     CDS             5427..6356
                     /locus_tag="JD016_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU5000973.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWGDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6517..6966)
                     /locus_tag="JD016_15175"
     CDS             complement(6517..6966)
                     /locus_tag="JD016_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000974.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARVLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7169..>8072)
                     /locus_tag="JD016_15180"
     CDS             complement(7169..>8072)
                     /locus_tag="JD016_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAU5000975.1"
                     /translation="LIVSYIFGKLVYNSSVGLEQLIKNEDIVKIFSERKDKPLEKLKN
                     YKTSELMIQSSNNYKLESLFITSNIKTRDTMILVHGIGSSYYEMLKVAYRYLDKGYNV
                     LVYNQRNTGNSGGDNYTFGLYERYDLDSLVKFVKNKFPQGRLGVHGFSMGAGTAAMHS
                     EINSKDDKVDFYILDSPYSEMKDAIRMGVLEKRIPDILINYVVTCGDLYNKFKSGFWY
                     SDVKPYESVEKSNVPILFIHGTKDTVCNYQNSKKMYDLVKHDKKDLWLIEGIGHVDGF
                     EHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2783 a         1339 c          982 g         2968 t
ORIGIN      
        1 ggaactggta aatctacttt attaaatata atatccgggc ttataaaaga atcttctggt
       61 caagtattat taaataaaca taacttgtct aatttagctg aacacagaag aactcaaata
      121 gtaagtagag tttttcaaga cccaagtctt ggaacttgcc cttctatgac tgttagagaa
      181 aacttgtctt tagctcttaa caaaggaaaa ctattaaatc taagaaaatg tcttcgttac
      241 aaaagagatt tcttagaaaa tttattggaa ggtgtatctc ttgatttaaa aaaatacctt
      301 gatgttcaag taaaattttt atcaggaggt caaagacaat cattatcact aattatgtcg
      361 tgcttaacaa atccatcagt tttattactt gatgaacata cagcagcact tgacccaaaa
      421 acatcaaatg aagtaattga attgactaat aagattgtaa gggaaaaaaa cataactaca
      481 cttatggtta ctcacaactt aaaacacgct cttcattatg gtgatagatt gataatgctt
      541 cataagggtg aagttgtatt agatgtaaaa ggtaaagaaa aagaacaatt aacagttgaa
      601 gaaatcttag aaaaatttga atatgcagtt taaaataata tatctattgt attaaactcg
      661 gctttaaaat aaagttaaaa ttacaataat tatggttact ttgtaataat tcatatgaat
      721 atatgaacat atataataat aaatttttga gggggatata aaaatgatta ataaaaaaag
      781 attggcaagt ttgattttag ctggtgctct tagtatatct atgcttacag ggtgttcaca
      841 aggaggagac tctggtaatt caaaacaaga aagtaattct aaagataaag aagttaaaaa
      901 aattggtata actcaactgg ttgaacatcc agcattagat gccactagga caggttttgt
      961 aaaagcatta gaaaagaacg gatttaaaga tggagaaaat attgatatag acttccaaaa
     1021 tgctcaaaat gacatgccta ctacacaaag tattgcgagt aaatttgcat ctgataagaa
     1081 ggacttaata tttgctatat ctactccatc agctcaggca gcatttaatg ctactaaaga
     1141 tatacctata cttattactg ctgtgagtga ccctgttgct gctggattag ttaaaactct
     1201 tgaaaagcca ggcacaaatg tatctggaac atcagatttt gtttctgtag acaaaggttt
     1261 agaacttctt aaaatatttg ctcctaaggc aaaaactata ggagttatgt acaatactag
     1321 tgaagtaaat tccaaagttc aagttgatgc tttaaaagaa tatgcatcta aaaatggatt
     1381 taaagttgtt gaaaaaggta taactacttc aaatgaagtt aaccaaggta tttctagttt
     1441 agttggcaaa atagacgtat tatatgtccc tacagataac ttagtggctt cttctatgcc
     1501 tatagtctct aaaatagcta ctgagaataa aatacctgtt atagcagctg aatctggtcc
     1561 agttgaaaaa ggtgctcttg cttgtcaagg tatcaactac gaaaaactag gttacaaaac
     1621 aggtgaaatg gcagttaaaa ttttaaatgg cgaatcagta tctgatatgc ctgtggctac
     1681 atcagatgat acagatatca ttgttaatga agatatatta aaagctttag gtatggaaaa
     1741 accttctaat gaaaatattt cttatgtaaa gactaaacaa gaatagaaag atattttcta
     1801 tatacgatta atatgcctgt ttctataaat taatttatag aaacaggcat atatttttat
     1861 tttaatgtat tatctattta aagattatat gtaaatgctc ttaatttgtt tgtataaatt
     1921 ttacttgtat aaactttata caatcatatt tattcttcta ttttctttat tactctacat
     1981 ggatttccaa cagctatagt atttggtggt atatcttttg tgactacact tcctgcacct
     2041 attactacat tatcacctat agtaattcct ggtgttatga ttactccccc acctatccat
     2101 acattatcac ctatttttac aggagaacca tactctatac ctgaatttct aagttgtgca
     2161 tcaattggat gataggctgt atagatttga acgtttggag ctaacattac attgtcacct
     2221 atttcaattt tacatacatc taaaaaaata caatcataat ttgcaaagaa attttcgcct
     2281 acatgtatgt tatatccata atcacaccta atgttttgtt ctacatttat ttgcttgcca
     2341 actgaaccaa atagttgtct taaaatatct tctctttttt catattcatc ctctaaagta
     2401 ttattaaaca atctagttaa ttttttacaa tactctcttt cttttactaa caattcatca
     2461 tttgcatagt atcctttccc agacaacatt ttttcttttt cagtcatata gtttactcct
     2521 ttttgttata attgtatcta aagcaaaata aatattcctg tggaaaatct aaatatattc
     2581 tgtcaccttt cttaaatctc attttacttt tttccaattc tatttgaata ggtacacatt
     2641 cgttagataa atcagttttt ctaacatata tagtatatgt aaaaggattt tctacaatgt
     2701 taatcaattc gaaacataac ttttcattta aatcttcatt agaatccaag accttaatat
     2761 gatgctccct gattcccaca tattctatat tatcacctat ttctctattt aatgtcaact
     2821 cgcatcccca gtctgtagca taaatagtat ttttatccag tctatttaat ttggatatat
     2881 ttttacaccc tgttatctta gcttctataa ggtactttgg agattcaaag agctcttttt
     2941 taggtctctt tggaagacct tctcctttgt tgtacactat aatatcatca caaactcggt
     3001 aagcttcttc aatatcatga gtaacaaata gtatatctcc cttgtaatct tttaacatat
     3061 tcataagttc tttttccata ttgcttctta aatgatggtc caatgctgag aaaggttcat
     3121 ctaaaagtaa tatatctgga gatgttgcca atgctctagc tagtgctacc ctttgttgtt
     3181 gaccaccaga taattgccaa gggtatctcc cctcaaaccc atttaattta agtatatcta
     3241 agtaccttgc tactatttcc tttttttcac tcttacttag ttttaaaagc ccaagttcta
     3301 tattttgact tacagtcata tgtggaaaca aagcatagtt ttgaaataaa aatcctacct
     3361 ttctatcttg tgttgataaa tttatcttct tttcagaatc aaatagaact ctatcattta
     3421 atacaatttt acctcttgtt ggtttctcaa gccctgctat acattttaat gtcatacttt
     3481 ttccagaacc agattctccc aaaaacccca gagtaccttt ttcttgttta atttctacct
     3541 ttagtttaaa agaggataaa tctttttcaa tatccacgta taagctcaac tatccacacc
     3601 tctttcctat aattttctgc tgaaattccg accaataatt tagtaataaa atcattgcta
     3661 tagatattgc tacaataatc ataacccaca tcatagcttt attcatatct ccgccttcaa
     3721 cagcaaaaaa tatagctatt ggcatagttt gagtttttcc aggtatattt ccagctatca
     3781 taagtgtcgc accaaattcg ccaagagctc tggcaaacga cagcaccaat ccaccaatta
     3841 ttccaggcca tgctaatgga actgcaatct taaaaaatac tttccattca ttaagaccta
     3901 atgttctagc agctgacagc atattattat caatctgttc aaaagctgac cttgaagttc
     3961 tatacatcat aggaaaagac accactactg ctgaaattac agttgctgtc caactaaata
     4021 ttaaagtcct atcgaaattt aataaaaact ttcctataaa tccattcttc ccacatagta
     4081 ataataaaaa gaaacctaca actgttggtg gcaaaattag tggaagtgta aatagtccat
     4141 ctataagacc cttccactta cctctatagt tagccataat atatgacaca aatattccta
     4201 ttacaaaagt tattattgtt gacaaaatag atgtcttaag tgatatccat aagggtgacc
     4261 aatctgttcc catctttccc tccttaattt taaatggaaa tagtgaaact aataactcta
     4321 ttaatttcac tatttccatt ttattataat acacaattat tttcaatttt catttattta
     4381 tatataattt atacttaatt acacataatt tatttctaag tttattctac ttttttatat
     4441 ccaaattctt caaaaatctt ttgtcctgac tcacttaata aaaattcttc aaatttctta
     4501 gcagcatcta cgtttttact agctttaatt actgaaactg gatatgtaat aggcgaatga
     4561 gttttttcgt ctgttttttc aactactttt atcttatcat tattgacagt atcactgaag
     4621 taaacgaatc caacatcagc atttcctgat tgcacccatg ctaaaacttc ttttacatct
     4681 tttgcaaata ctagcttatc ttttaactta tcttttaaat ttaaattagt taacacctca
     4741 tctgcatact taccagctgg aacactttca gcttcaccaa cagcaatttt cttcacttta
     4801 tctgtagtca aatctttcat acctgataca ctactgtcag cagaagaaat aagaactaaa
     4861 tcatttttta ctaaatcttt catagtatca gaaactaata acttctcttc atccaaagct
     4921 ttcatttgtt tttgacctgc tgaaataaat aaatcacaag gtgctccttg ttctatttgt
     4981 tgttgtaatg agcctgatgc tccatagtta actgtaagtt ttacatttga atcaactttt
     5041 ttatattctt cttcaatctt tgccattgct tcttttaaac ttgcagcagc cgatatattt
     5101 aactctactg aatcactact tttagttgtt ttttcttgtt ttccatcatc ttttttgtct
     5161 tcattactat tgcacccaac tgtccccaaa gttaaacatg caactaatcc taatattccc
     5221 aatatttttt tcataacaat ctcccttttt gtaaatttga tacacatttt aacatgtttt
     5281 attatgtttt atcatgtttt gttatatata gtttaattat atatacatga taataaattt
     5341 tcaagtaatt tatttacact acttttaaat attttgctac attttccttt ttttaatgtt
     5401 aaaataacct tatatggagg tggattatgg actcattatc atctttgaca cctgtcgagg
     5461 tagctgaatt attaaagata acaaaaaata ctgtatatga attaatcaaa agaggtgaac
     5521 ttccttctta taaagtaggg aaaaaaataa gagttgatac taaagatgtt gaagaatata
     5581 tcaacagtca aaaaactggt aaagtaaagg atacacattt caacaataaa aatatagata
     5641 taaataataa tttcaaaatt attatttctg gtcaagatgt aatattagac attttagcta
     5701 ggtctgttga aaaaaaattg gatggaataa gtacatttcg ctcttatata ggtagttata
     5761 atgctttgta tgagttatat aatggcagag tttctatagc atcatcccat ctatgggatt
     5821 tcgaaacgga tgaatataat agcacttttg ttaaaaagct attgccagga ataccatgtg
     5881 tacttataaa tctagcttat agaatgcaag gtttctatgt tgctaaagga aatcctaaag
     5941 agataacaac ctggggggat ttaacaaaac caagtataac cattgtaaat agggaaaaag
     6001 gttctggaac tagggtttta ctagatggta aacttagact tctaaacttt aatggtaaac
     6061 atataaaagg ttatgataat gaagaattat cacatcttgg agttgcaagt actgtttctc
     6121 gtggaatagc agatgttggt ttgggaaatg aaaaagctgc tttacaagta aataatatcg
     6181 actttattcc tcttcacaaa gagcgctatg atttagttat taaaaaagaa gacttacaaa
     6241 atccagttta ccaaactata attaacataa taaactctcc tgagtttaaa gctgaacttc
     6301 aaggtcttgg cggatatgat ttaactgata ctggaaaaac tatagcaaaa gtttaagatt
     6361 aaaatcgcaa tctattttat aagatttata atttttcaca aaatttataa cttaaacacg
     6421 aaataaattg cgattttagt gtctcaaaaa atttatgtct ttcattttat catccaatac
     6481 aaaatttaat ataattaatt gcgcaatcat actatattaa tatatattta cataatagta
     6541 tttttcttta atgtagatta acatatgata tgttttaaac atcaataccc ttgctttctt
     6601 atatatatgt ggcaacacat atgagtttaa taaattatat acaatgttgc tttcttcttg
     6661 tattttttca accaacaagt tatttataaa attacctttc tcattttcaa taatctcact
     6721 ccaatttatc ttaactttta atatatatct tcccactagg tatacacact ctacaataaa
     6781 taataaaaat acaatgcagt cagtacctgt tgtcttatat aaattacccg atactaatgg
     6841 ttttgcgaag aaattataaa aattcaaagt atcacccgta taatcaaaaa gtattaatag
     6901 cactcctaga actctcaata ctttaatatt caacatgttt ttatctttat tatttttgtt
     6961 tttcatatat actacctctc ttttctttag atttgattgt aatctaatac taactctttt
     7021 atttgaggta taaatgaatt taattggtag ttattttcat ttttactaga aattaattca
     7081 cattactaaa caaaacttat atttaaacat aaaaatagcc tatctatttt ttaataaata
     7141 caaaaataaa taagctacta atattcaatt aacttttatc acttaaaaca ttactatcta
     7201 taaatttaaa tattttattg aagtatactg tactatcatg ttcaaatcca tcaacatgac
     7261 caataccttc tataagccat aggtcttttt tatcatgttt aactaaatca tacattttct
     7321 tgctattttg ataattacaa acagtatctt ttgttccatg tataaacagt atgggtacat
     7381 tagatttctc aactgactca tatggtttta catcactgta ccaaaatcca gatttaaact
     7441 tattatataa atctccacaa gtcaccacat aattgattaa tatatctggt attcgttttt
     7501 ctagtactcc cattctaatt gcatctttca tttcagaata aggagaatcc agtatataaa
     7561 aatcgacctt atcatctttt gaatttatct ctgaatgcat agcagctgtc cctgccccca
     7621 tggaaaatcc atgcactcct aatctccctt gaggaaattt attttttaca aatttaacta
     7681 gactatctaa atcatacctt tcataaagac caaatgtgta attatctcca ccactattac
     7741 cagtatttct ttgattataa actaatacat tataaccttt atctaaatat ctatatgcta
     7801 cctttaacat ttcatagtaa ctacttccaa ttccatgaac aagtatcata gtatctcttg
     7861 tctttatatt tgaagttata aaaagacttt caagcttgta attattagaa ctttgtatca
     7921 ttaattcaga tgtcttgtag ttttttaatt tctccagtgg tttatctttt ctctcagaaa
     7981 atatttttac tatatcttcg ttttttatta actgctctaa accaactgaa ctattataca
     8041 ctaatttgcc aaatatataa gatacaatta at
//
LOCUS       DACXMZ010000183         7951 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.184, whole genome shotgun sequence.
ACCESSION   DACXMZ010000183 DACXMZ010000000
VERSION     DACXMZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.184"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(264..1424)
                     /locus_tag="JD016_15185"
     CDS             complement(264..1424)
                     /locus_tag="JD016_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAU5000976.1"
                     /translation="MIMINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFER
                     GIILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYL
                     LLVALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFS
                     YFGLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLL
                     YSMVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKL
                     SAIMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLI
                     ALSIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(1421..1897)
                     /locus_tag="JD016_15190"
     CDS             complement(1421..1897)
                     /locus_tag="JD016_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAU5000977.1"
                     /translation="MSIMFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFA
                     MIAKDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQ
                     GQEVNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(1902..2390)
                     /locus_tag="JD016_15195"
     CDS             complement(1902..2390)
                     /locus_tag="JD016_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000978.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSSF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIRQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(2404..3735)
                     /locus_tag="JD016_15200"
     CDS             complement(2404..3735)
                     /locus_tag="JD016_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HAU5000979.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGSIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(3749..5314)
                     /locus_tag="JD016_15205"
     CDS             complement(3749..5314)
                     /locus_tag="JD016_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS alpha-glucoside transporter subunit IIBC"
                     /protein_id="HAU5000980.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(5484..6269)
                     /locus_tag="JD016_15210"
     CDS             complement(5484..6269)
                     /locus_tag="JD016_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAU5000981.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(6436..7650)
                     /locus_tag="JD016_15215"
     CDS             complement(6436..7650)
                     /locus_tag="JD016_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAU5000982.1"
                     /translation="MDENYMKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFN
                     YVYNVVKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNK
                     HTEVIDNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILK
                     DVIKGQIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQ
                     GIVSKLIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKD
                     FNDFEDTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGD
                     GGCIDPVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNC
                     IRKALELENINY"
BASE COUNT         2826 a         1347 c          808 g         2970 t
ORIGIN      
        1 tatataaata aatccttaag taaatattac attttaactg ttagcattac acttttgtaa
       61 catatggaag actattataa ttataatgga aatatcttaa aataaaagca tgatttactt
      121 ctttgtataa acaaagaaaa gaagctccta agaagcctct aatctataca attattcttc
      181 tctataaaaa accaaaatac acattgattg tggcaaatac agatactata aacaagtgat
      241 attactacta tatattaaaa caattattcc tgaagataat tcaataagac tatcaagcaa
      301 taagctaagc atccaaaaag acttaatacc attaatgtat aattataccc aatattaatg
      361 ctcaaaaaac cagcaacagt tggaataatc agagatgaac ctcccataaa tatacttaaa
      421 gctattaatt ggctactact tctaagtttt ttaggagaaa cttcaaatat taatcttttt
      481 gaagtaatta aaattaaagg agtagaaaac acttgaaaaa cacacagtat aataatgata
      541 tttgaatcat ttgttaaagc aaataataga aactgaactg ttatcataat agcacttaat
      601 tttaataagt ttaaactgct atattttttt aataaataag aaccagccag atatgttggt
      661 atctctaaca atgactgtaa agaccacttt aaagatatct gagaatttgt agcatttaaa
      721 gctatcattt tatctattac agtacagtta tttgctacca ccattgagta taataagaat
      781 aaaatacata ctagtaagat atatttttta tcttttaata attcaataat atctttatta
      841 gttacattta ttttcttatt aatcttttca atatcaggta ataatagtat atttataata
      901 agtaatacta caattaaacc aatcgctaca gataatccaa aatatgagaa tctaagaatc
      961 aagtaagatg ctaccatact tccaatagcc catccaatcg acccaaaagt cttaataaat
     1021 gacaaataat tattaatatt atctccacaa cctattatcc atgtatcata taaactacaa
     1081 caagtattta ataatcctcc tgataaagca acaagtaata agtatattat aaaagttgca
     1141 gattcagtag aaaacataat aactgatgca attccataaa ctgataaact aattaaaaca
     1201 atttttttca ttgtttgata cttatcagct aatactccaa ataataattg aaacaatata
     1261 ttggttattg catagctcga taatataatt cctctttcaa acgctgagta acctaatttt
     1321 gataagaaag gtgtgaattt tgttgaagca aaggcaaaaa taatatatga gaaaaaatag
     1381 aatatgataa atttaaattt aatcgcttta ttaatcataa tcaatcaaaa ttgataactt
     1441 taataccact atttccttcc tgatttaagt ttgaaagatg atattttacc gtattaggat
     1501 tctctacaat aactaaagga atatataaag tattttcaag taacttatta tcaatttcta
     1561 ctaacttttc ccccatatta acttcttgac cttgtttaac ttctaattta aagttttctt
     1621 tttttgttgt tgcactatcc aatccaatat gaataagaat ttgaacatca ttttgcaatt
     1681 taattcctat agcatggtta gtttgagcaa taaaacttat tactccttta gtcggtgatt
     1741 taagaatatt gtcctttgct atcatagcaa atccatctcc cattacctta gttgaaaata
     1801 ctttatcagg aatttctcca agattaatta tttttccatc aatgtatgaa ttaattgtaa
     1861 ttgcttcttt ttttctttta aagaacatta tagacactcc tctacataat taactttatt
     1921 ctaattatca ttacaaataa cgatattaag catactgcaa ttgaggaaaa aagagcctta
     1981 tagctctttc cccattgtag ttctttaata ctgcttatga tttgtctaat tccaacaaat
     2041 ttataaatat aggtaaacat gataacaata gttattgtgt aaatttggaa tatctgccct
     2101 aataagacta aatagaaagc aatcatgttt tcttttatct ctttattctt ttttatattg
     2161 taaataacat atgcactgta tactgcacta aagttagaga ttaaatttaa acttgcatta
     2221 acatcattgt ttaaaagttg tgaaaatgaa atatttgtaa atgatgatgt caattctaaa
     2281 tacactatat ataaaacagg taatattaga aaacaaacca taaccactga aagaaatata
     2341 ttttcatctt ttaatactgc tgtgatattt tttattttat ttgttatcat gtttttgttc
     2401 tccttattta aaagttggcc agaaatcctt attagccaca atcatttcat ctaagatttc
     2461 ttttgctact tttgcacttg gaacagtttt actcatagta aacgactgcc ataatttttg
     2521 ataacttttt tctatccatg cttctacagc taatttttca gaagcaactt gttgttccat
     2581 taaacctttt tggaaagttg gaattttacc tatagacata ggttcaacca tatcttttcc
     2641 aacaaggcaa ggaatttcaa ccattgcatc atctgcaaaa ttttcaatag ctccattatt
     2701 aggaacaatc agtaaaaatc tttcctgagt attaaatgct aatgcacaag ctaattcaac
     2761 aataaattga gaatgttcac ttgcatcaaa ccatgtatct cttgctgtgc cattttttat
     2821 aattctttca cattcttcga atgtatcctt ttctcttcta tccataattt catttgctct
     2881 tgtataattt ggattagcat gttcaacagt ttcatcagcc attaaataat attttaaata
     2941 agtattaggt aatgtagttg gttctatttc taataagtct tttgctgctc tcatagtttc
     3001 taaccaacta gattctttgt gttgaccttg aggtgtaaat tcctcatatc cttttttagc
     3061 acaatattct tgtaatttag gcattaaatc atttccttct ttgtccttaa tacttgtcca
     3121 ccacccgaaa tgattcaatc cataatatct aacatcaaag tctttacgag aatttaaacc
     3181 taaaactcta gcaaatattt cctctaaaca tactggcata tcacaaatat taataattct
     3241 tgaatttggt cttaatacac gacatgcttc tgcaactata gctgctggat ttgaataatt
     3301 aagcatccaa gcatttggag aatatttttc catataatca atcatttcga ttattgaacc
     3361 tattgttctt aatccatagg caattccacc tgccccacaa gtttcttgac caacaacccc
     3421 atattttagt ggaatttttt catcttgttc acgtaaagca tataaaccaa cacgaatttg
     3481 tgccattaca aaatcaatat cagtaaatgc ttcttctgga tttgttgtat aactaaattt
     3541 aatttctgga tttttctctc taacaattat tgcacatgct tcagcaactt ttctttgtct
     3601 ctcatcatta ttatcatata atttaattga ttcaatagga aatttagcaa cactttcaat
     3661 taacattgtt acaattccag cagtataagt actaccccca ccagcaatta gtatcctctg
     3721 tctttttctt tccatctttc cttctccttt ataataaatt ttcaaattct tctcttacac
     3781 ttggtactga aagcccaata ataatctgaa tagcttttcc attatgcact aaaccatgtg
     3841 cacctactgc cttaaaatct tcttctttag aaataattga gtcatctttc aaggttaatc
     3901 tcaacctcgt agcacagttt gtaacatcta caacattttc tgctccacca actaatttta
     3961 tgtattcaga tgcttgttga gaggctttac tcaatttatt tcctttttca tctttagttt
     4021 ctaagtattc atttttagta tatagttttg tttcttcact atcttcctct ctaccaggag
     4081 ttttcatatc aaacttctta attaagaaag taaatacaaa gaaccagata aatgaaaata
     4141 taatccctac aacaacttga acaacatata ccttccagtg atacattcca agaggaagcc
     4201 aatttaatac aacccagtta ataattcctc caccaaaatc accaacaact cctagggcaa
     4261 acgaaatcgt ttgcaagcat gcatctaaaa atgcatgtac taggaataaa agtggtgcta
     4321 caaataaaaa tgtaaattct aacggctcag taattccagt taatactgca gtaagagtag
     4381 caggaatcat taaacctaat acttttttct ttttttctgg ctttgcagtt acatagaaag
     4441 caccaaatac acctaatgaa ccaaacacct tcgataatcc ggataatgaa aatccgattg
     4501 gatacatttc ctttagagtc ttagctgaag tttgaaaatc ctgaatatga gtagcccaat
     4561 atactgatgt accacctgga acaacaacac tatcataaca aattggtgca tatacaaaat
     4621 gatgtaatcc agttggtatt aaaatctttt gtaagaatgc ataacaccaa actcctatat
     4681 ttccactatt tataaaaaac gtttgcaaat gtctaataac attttgaata tgaggccaaa
     4741 taaaacaagt taataatgca actggtatca taacaaagaa acaaattgca actacaagtg
     4801 atgagcctct aaataatcca ataaaatctg gcagttcttt atcataaaat ctattatgaa
     4861 tataaatgac aatacctgat attaacaaag caccaaccat accagtatct aatgttttaa
     4921 taccagcaat gcttacaagt ccacttccag cacttatttc agctgcgaaa tcaactccaa
     4981 agctttttcc ccaaaaactt aacatagaac ctacaaaata attaaaagtc aaatacgttg
     5041 ctaaagcctc catacaagct cttgcttgtt gcttttttgc aagtgctatt ggtaaagaaa
     5101 tagcaaataa taatggcaat tgattaaata ccatccaagc tccactagat attacacccc
     5161 aaaattggta ccaaatattt tctggtgtag caagtgaacc cataattgac tgattttgaa
     5221 atacaattga gaatccaacc attatcccag caaaagcaaa taatagtgtt ggtgtaaaca
     5281 ttgcccctcc aaaacgctgt attttcttca tcatataaat tactcccctc ctcatatttt
     5341 tagtttatct ttggccttag atatctatta ttttaacagg ttaataaata tatacaatct
     5401 ttagggtaca ttcggagctt attcacagtc aaataagttt ttgcacattt ttatgttcaa
     5461 attctcataa aaaaaaagaa tgactacaat ttgcaatcaa tcccttctgt aatcattcta
     5521 tgccttttat aaatactata tttaataaat aataactcaa tcaatataaa atataacatt
     5581 gtcaatttat aactagggtg catttgtaaa aatgagatat tagtcgctga aatataaatt
     5641 gattcatcac ttaaatcagt caatgtatta tttttaaact ctgtaattga aattatttta
     5701 gttcctatta attttaatct tctagcaata ttaagtgtat tctcattctc accacttagg
     5761 gatataataa tcataacatc atttttactg acaatattgt aaaaagaatc atcaaatgtt
     5821 cttccctcaa aatcatatat tatttcttga catgataaga acattctctt tatctgtttt
     5881 gcaaccacat tttgaatatc acctgaacca taagcgtata ttgttcttga ttcataaaga
     5941 agtttacaca atgcatcata atctttattt ttttgatctt caacatactt aattattgaa
     6001 ttacatgcaa tatcaaatga ctgattttct atgacatgaa ccttttctga ttcccagctt
     6061 aataatactt taaactcact gaaacctttt aaatctaatt ttttaacaaa tcttgtaata
     6121 gttgcacgag acacattaca tttcatagca agttcattta ttgacatctt actacaatgt
     6181 tttttatttg cagttatata tttccatatt aataaatctg tatcattcaa tttattataa
     6241 tttaaaaaaa tcatttcttc taatctcata aactattacc ccctttaatt aaaaatcgtc
     6301 ataaatatta taccaatatt caacattaaa ttacagcgta ttcttatatt tactatttag
     6361 cccataaaat tgactataaa aaatactgct ataattgatg tttaaaaaaa tgtagtttta
     6421 attttattct tttatctaat aattaatatt ttcaagttct agtgcttttc ttatacaatt
     6481 atcatcttta aaatcagcag tccttttaca atcttttact ttaaattctg gaatagtttt
     6541 aaattcctca tcacatattc cattcttatt taaatacata caacttgcca ttctaactat
     6601 tagaccacta ttttttaaat taaacaatac agggtctata caccctccat ctccgccagt
     6661 agtttcacca actaaagtcg caaatttatt ttctttacaa aacattgcaa acgactctga
     6721 tgatgaatat atttttctat caacaagtaa gtatatatta cccttaaatt ttgaatcatc
     6781 tgtagatttt atagtatagg aagtatcttc aaaatcatta aaatctttta taatttcttt
     6841 aggtgcattt tcctttacac ttgctggtaa attttctaca ggatttaatt ttatatttct
     6901 tgcatttgta tattttttta ctatttcatt attatttcta taaattctat aaccatttat
     6961 tttgacatca ttttttatga gtttagatac gattccctgc caatacctat cacttccacc
     7021 taaattacct cttatatcaa taattaaagc tttatgtttt tctaaagtgt taatataatc
     7081 tccaattatt tttaaatcat tatttataga gccattcttt gatgacattg atggtaaata
     7141 catatatcca atctgaccct taataacatc ctttaaaatt aattcttttt ttacaaaaat
     7201 atcatctggc atttttattt taagattcat agagtcatat ctatccacaa cttttttatc
     7261 attaagaaaa tcataccaat cattattaga gtatgatttc ttaaaaagtt catatctttt
     7321 cttattatct attacttctg tgtgtttatt atttaaatca gataatatag atgacagttc
     7381 ttctataaat tcttcatcat tttcagtgtt ttttattcgt tttgtatatt catccttatt
     7441 tgctaaccaa tcaatattat taagcctctt atttacacct agatatggat atcctgtctt
     7501 tacaacatta tacacataat taaagtcatc caccttttcc tcttcagaca actgattctt
     7561 ttgagaataa gtagaattac ctaataatat tataaatgct aaaataatta ctaaaaataa
     7621 tactttcttt ttcatgtaat tctcatccac tttcagattt tatattttaa tacattgtat
     7681 cattatttta ttatcaccta aacagttaaa agaaattctt gaagatatat tccactatta
     7741 ttctatttaa aaatttttat ttgtattttc aaaattattt catgaaagtt ataaaataac
     7801 aattatattc aaatatctat tttactgcta tatcctgaaa gtaaattctt atcagtgata
     7861 tactaaaaaa gatatgctaa aaaatttatc cattataatg acttaaattt agatacttta
     7921 ctgattttta tttattagtt gagaaagata t
//
LOCUS       DACXMZ010000184         7941 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.185, whole genome shotgun sequence.
ACCESSION   DACXMZ010000184 DACXMZ010000000
VERSION     DACXMZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.185"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            309..764
                     /locus_tag="JD016_15220"
     CDS             309..764
                     /locus_tag="JD016_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAU5000983.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSSLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            813..1397
                     /locus_tag="JD016_15225"
     CDS             813..1397
                     /locus_tag="JD016_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HAU5000984.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1402..1725)
                     /locus_tag="JD016_15230"
     CDS             complement(1402..1725)
                     /locus_tag="JD016_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5000985.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSN
                     YLENL"
     gene            1877..2395
                     /locus_tag="JD016_15235"
     CDS             1877..2395
                     /locus_tag="JD016_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HAU5000986.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2572..3240)
                     /locus_tag="JD016_15240"
     CDS             complement(2572..3240)
                     /locus_tag="JD016_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000987.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3355..4770
                     /locus_tag="JD016_15245"
     CDS             3355..4770
                     /locus_tag="JD016_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAU5000988.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTILDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKEFSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4969..5451
                     /locus_tag="JD016_15250"
     CDS             4969..5451
                     /locus_tag="JD016_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5000989.1"
                     /translation="MKTNTDVIMEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFT
                     NVVGCTIHQYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQ
                     VYRNKDEFYPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA
                     "
     gene            5448..6032
                     /gene="clpP"
                     /locus_tag="JD016_15255"
     CDS             5448..6032
                     /gene="clpP"
                     /locus_tag="JD016_15255"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAU5000990.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6344..7420
                     /locus_tag="JD016_15260"
     CDS             6344..7420
                     /locus_tag="JD016_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAU5000991.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7487..>7941
                     /locus_tag="JD016_15265"
     CDS             7487..>7941
                     /locus_tag="JD016_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HAU5000992.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGAT"
BASE COUNT         3027 a          930 c         1331 g         2653 t
ORIGIN      
        1 ttaagattat gagttttata attttgatat agggatacta ttttcataaa tattacaaca
       61 tgtagaagta tatgtctagt attgttgaat tttcaatgat atattaaggt tctaatatat
      121 tatacaaaat aaaaacatta attcaactta aataatttta agtattatat gtgttttgat
      181 taaagaaaac taaaatttta aggaaaaata acatatttgt attaaatttt attgaaatga
      241 ttaaatttat ttacttaatg taaattatta gctaaaattg tattaaataa ttaaggaggt
      301 gagtagctct ggatgaaata gatttaaaaa taattaattc tctaaaaaag aatagtaggg
      361 cttcaacctc tgaaataagc agacaaatta atttatcagt accagcagtc tcagagcgta
      421 taaggaaaat tgagcagtca aatttaattc aaaagtacac tattcaaatt aatcgggaaa
      481 aaacaaaata taagcttttg gcttttatat ttatcaatac tgatttatct aaaagtatga
      541 aagaatttaa gaaaactata ttggaatatg atactgtatt agaatgtcat catatttcag
      601 gagagtatga ttacttaata aaagtattag cagaagatac aagtagtctt gaagagttta
      661 tatcagtgac tttgaaggaa attgggggga ttcaaaaagc taatacaacc attgttttat
      721 ctacaattaa agaagaattt aatacaataa aagatatttt ttaaataatt tactagtaaa
      781 gtgttaatta tattttagga ggaaaataga atatgaaagt tttagttttt ttagcaaaag
      841 gttttgaaac tatggaattt agtgtatttg tagatgttat gggatgggct cgtaacgatt
      901 atgggcatga tattgatgta gtgacttgtg gatttaaaaa acaagttatg agtacattta
      961 atatacaagt attagttgat aagacgatag aagaagtatg tgttgatgat tatgatgctc
     1021 tagctattcc tggaggattt gaagaatttg gattttatga tgaagcatat gattcatctt
     1081 ttttaaatct aataagggag tttaattcaa aagaaaaaat aatcgcttct atttgtgtgg
     1141 cagcattgcc agttggaaaa agtggtgttt taaaaaatcg taaagctact acatatcatt
     1201 tgaaaaatgg taaaagacaa agacagttaa gtgagtttga tgtaaatgta gttaatgaac
     1261 caattgttgt agataaaaat attataacct catattgtcc agaaacagct ccacatgtag
     1321 cttttaagct tttagaaatg ttgacatcta aagaacaaat ggatgaagtt aaattagcaa
     1381 tgggatttaa actgtaatgg tttatagatt ttctaagtaa ttagaacccc agtcacacat
     1441 ggaatctaaa attggaataa tactctttcc aaaatcagat agcgagtatt ctacttttgg
     1501 aggaacaaca ggataaacca ctcttataat taaaccatct gattctaatt cacgtaattg
     1561 ttgagttaac atcttaggcg ttgctttagg aattaatttt tttaattcat tgaacctcaa
     1621 tatagtgtct ttaagatgcc aaagtataag tgtcttatat ttaccaccaa tcaatgcaag
     1681 tgttgcttct attgggcaac tgtaattatc taaacatttt ttcatttttg ggctccttta
     1741 tttattttaa agtatctttt taggtactat attacaaaaa agtgcattat tgtaatatag
     1801 taccatagtg ataaaattat agcataggtt aaataaatca tcaacttagc ctaataaaac
     1861 aatagggggt aaataaatga atttcgttga acttgcaaaa aaaagatatt cttgccgtaa
     1921 ttatcaagat agaaaggttg aaaaagaaaa acttgagaag gttttagatg ttgcaagaat
     1981 agctccaact ggtggtaatc gtcaacctca aagattgatt gttattcagg aaaaagaagg
     2041 tataaataaa ctttctaagg ctgccaatat atatgatgct ccattagcaa ttcttgtatg
     2101 tggagataaa gacaaagttt ggacaagacc ttttgatggg aaacaactta cagatattga
     2161 cacaagtata gttacagacc atatgatgtt acaggcaact gaacttggat tggcaagtgt
     2221 ttgggtgtgc tattttaatc cagatataat tagagaagag tttagtttgc cagataatct
     2281 agaaccaata aatatattat tgatgggata tgaatctaag ataccagaga gcccagagag
     2341 acatgaaaaa actcgtgttc ctttaagtga aattgtttct tatgaaactc tttaattata
     2401 aaattaagtt attcattagg ataaatgtaa taaagtgttt attataaaca aaattcaaaa
     2461 taaataatat ctacatataa ttaattttag tgaagtatat ctagtttaac tagaatgtat
     2521 acttcactaa tttatttatt caaaatattt atgaggattt taatatatta actaaaatat
     2581 atcaattgaa ttaactttta aatctggaaa agcacttgaa atatagtgtt ttatgtcatt
     2641 taaatggtct tgaatttgct tttctgatat tttatcaggt ccatatacaa aatacataat
     2701 atttttagta tcttttgtta tagctgttcg attatcaggg ccattaagaa tactagataa
     2761 tataccaata ttatctgata aaaataaatc atcttttgtt aaaatatgtt ctttaccacc
     2821 tataccataa aaatgttcac ttccatttgc taattctatt ttcaaaggta attccatttt
     2881 atcaaggtca tgacctgctg ttagaagaag atttttcaat tctgctaaaa acattgactc
     2941 aattgttaca cctacatctg ggataccttt tgattttaat agaatagatt ctaattgaag
     3001 caatacaggg taagttttct tgaatttttt ataatacttt atataacttg aaacaggctc
     3061 tgttttaatt ttttctttac gattgtattc tgggtgttgt tctataatat ttttaattgt
     3121 agagttttta agaactaaaa aattttcttt attaggtttt gaatgtgtaa tatttataac
     3181 cataactcca aatttaatat tgggataaat ttctttaact ttatgtgata tttctaacat
     3241 aatatagact cctttttatt ttatatttta tgatataatc ttagcaaatt aaaagtggat
     3301 tttgtagttc catttttata gtaattttat agaaccactt ttggaggtat tagaatgtgg
     3361 attacgatta atagaaatag taatatatca ttatcaagac aaatttattg tgagataaaa
     3421 gatatgattc tagatggaag cttaaagttt gagcaaaaac tgccatcatc taggacttta
     3481 tcaaaagaac tatctgtatc tcgtaataca attttagatg catataatca attaattgca
     3541 gaaggatatt tggaaacaag gaatggatat ggaacaattg tagctaaagg aatatcaggg
     3601 cataaagttg attttaagtt aaatgatact tttagagatg aaaaagattt acatactaat
     3661 aaacaaaaaa attatataga ttttcgttca ggagttccag aattaaaact atttccacgc
     3721 aaagaatttt caaagctata taaacaaata ctgtatgatt tatcagattc ggatttcaga
     3781 tatggaagta cagctggtgt atgggtttta agagtggaaa tatctaagta tttatttcgt
     3841 actagaggaa ttagatgtaa tcccaaaaac attatgattg tttctggttc aacacaaggt
     3901 ttatctttaa tttcacagct tttatacaaa agtaataaaa aggttgctgt agaagaccct
     3961 attcataaag gcttattaaa ggttatttca tctattggat attcagttgt tggaattaaa
     4021 gtagatgata agggaatgaa taccaactta ataaaatcaa taaaagggat ttcttttgtt
     4081 tatactacac cttctcatca atacccatta ggagggattt tacctattca aagaagacta
     4141 gagcttatta aatgtgcaac agagaatgat tactatataa ttgaagatga ttatgatagt
     4201 gaatttagat atgagggtca accaattagt tctctttatg aacttagccc aaataaagta
     4261 atttacatag gttcttttag taaaatatta tctccagcta ttcgtgttgg atttatgtta
     4321 ttaccagaca atattttaga aagctataaa accttaaaaa tgtactctga tgttcataca
     4381 gaaacaatat tgcaacatgt attagctgaa tttattggaa atggaggttt agagaagtac
     4441 atatttagaa tgaagaagtt gtacaataga aaacgtaagt acctcataaa agttttaaac
     4501 acggaattta aaaatgaatt taaaataaaa ggtgaagcag cagggcttca tatagttgct
     4561 catttctata atgttaaatt tacggaaagt ttaattcata aaatttttga agcaggtata
     4621 aaagtttatc ctatacaaca atatgcaatt gagaatatat tagaatatga ccatgaaatt
     4681 atactaggtt atgctcatct tagttttgat gaaattgaaa aaggtgtaaa aatactcagc
     4741 aaaataatac atatgaatca aggtacatag ataaaacata tagcaatcta catataatag
     4801 atgtaattaa gtttaaactt taaaagaaat atatattaat atttttgtat aaaaatacct
     4861 gatatttttt tatcgatact ttattgctat acactaaatt aagatagcaa tttatattaa
     4921 gaaaaaactt tttgcatagg ttataatact taaaaggaga ggatagagtt gaaaacaaat
     4981 acagatgtta ttatggagat aattgattat atagaagaaa atcttacaga aaatctaaat
     5041 ttagataaaa tagcttatat tgctaattat tcaaaatacc atttacacag aatgtttaca
     5101 aatgttgtag gatgtactat ccatcaatac atacagagga ggagacttac tgaagcagct
     5161 agacaactta tatttacaga caaatatatt atagacatag ctattacagt aggatattct
     5221 acacatcaat cttttacact agcatttaga aatttatata aagaatctcc acaagtatat
     5281 agaaataaag atgaatttta tcctattcaa cagagatttg ttttaaatct aaatcatcaa
     5341 attcaaagcg aaaaatgtat ttcttatgca aatatgaatt ataaacaaag aagagatgaa
     5401 atcaattctt atcttaactt taatattaat tgtgaggtga aagtagcatg agtttagtgc
     5461 cacatgtaat tgaacaaaca ggtcaagggg aaaggtctta tgatatatat tcaaaattat
     5521 taaaagatag aattatattt ataggagaag aaattaatga tgctattgca agtttagtag
     5581 tagctcaatt actgttttta gaatcagaag acccagattc agatataatt atatatataa
     5641 atagccctgg tggctctgct acatcaggat ttgctatata tgatactatg aattatgtca
     5701 gatgtgatgt atcgactatt tgtattggta tggctgcaag catgagtgct tttttacttg
     5761 caggaggaac acatggaaaa agatgtgctt tacccaattc agagattatg attcatcagc
     5821 caatgggagg agctaaaggt caagcaacag atgtaaaaat agctgtagat aatatattga
     5881 aaataaaaga acgattagat aaaattcttt ctgaaaatac aggtaaatca atagaagata
     5941 ttaggagaga tacagataga gataatttta tgactgcact agaggctaaa gagtacgggc
     6001 tgattgatta tataatgaat aggaatgaat aaagtatcaa agtatcatat taaaatgtct
     6061 gttttacaag tatatatata atattaaata agagtgtctc aaaatgaact ttttaattta
     6121 tgggacactc ttttttgtat gaaattaaat atggttttat catttcaaca attattacca
     6181 aatttcaagt ttaataaatt ctactataaa aaatacataa aagataaggt tctttagtaa
     6241 ctttctgtcg tcaaaagttt ttataaattt aataacaaaa caagagaaaa taccatatat
     6301 tacttatgtc tgcaatatat agaagacaaa ggagttgtac attatgatta caataagttt
     6361 gtgcatgatt gttaagaatg aggaagaagt tataggcaga tgcttagaat gtgtaaagga
     6421 tatagtagac gaaattataa ttgtagatac cggttctaca gattccacaa aagaaatagt
     6481 ctcaaaatat acagacatgg tgtacgattt cgaatggata gatgatttct ctgctgcaag
     6541 aaatttttca ttttctaaag cttctaaaga ctatactatg tggcttgatg cagatgacat
     6601 aattttagaa gctgatagag aaaaattatt aaagattaaa gaaagcttgg atacttccat
     6661 tgatattgtt atggcaaaat acaatgtatc ctttgatgaa aatggtaacc caactctctc
     6721 gtattataga gaacgattgt ttaaaagaag ttctaatcat aaatgggttg accctattca
     6781 tgaagtagta cctctttttg gagaggtatt ttattctgac attgcaatat ctcataaaaa
     6841 gcttcataga caggaccctc taagaaatct tagaattttt gaaaaaatga tttcagaagg
     6901 aaaaacattg gagccaagac accaattcta ttattctaga gagttgtact ataatgctag
     6961 gtataaagaa gcaatagaag gttttactaa gtttttagac tcatcaagag gatggattga
     7021 ggattgtatc agtgcttgca gagatttagc cacatgttat tacttaatta atgatgaaaa
     7081 aagtgcctta tattcattgt ttagaagttt cgaatttgat gagccaagag cagaaatttg
     7141 ttgtgacata ggaaagcata tgtttgatag acaaaaatat aaagaagcca ttttttggta
     7201 taaggtagca ttaacaagag ataagaatga tactaatgga ggttttaagt caaatgattg
     7261 ttatggatat ataccatcta tacaattaag tgtctgttat gacaggttag gagaatcaga
     7321 taaagctatt tattatcatg aaaaaacaaa agaaatcaaa cctaacgata gtgctgtttt
     7381 gcacaatgaa aattatttta gtaaatttaa aaatagttga taggtcacaa tcatactaaa
     7441 tttgacatat tatatataga gtttaaaagc tcaaatatat caggaggtgc ttttaataat
     7501 gagtagaaat aaatattttg gaccatttga tgataatgat tacaacaatg gctatgataa
     7561 atatgatgat tgtaacaatg gtcgtgatga ttataatagc tgtgattgcc atcattgctg
     7621 tccaccatca tgtgtaggtc caacaggccc aatgggtcca agaggtagaa ccggcccaac
     7681 aggtccaacg ggtccaacag gtccaggagt aggggcaaca ggcccaacag gaccaaccgg
     7741 tccgactggc ccaacaggaa atacagggaa tacaggagca acaggattaa gaggtccaac
     7801 aggagcaaca ggggcaacag gcccaacagg agcgacagga gctatagggt ttggagtaac
     7861 aggtccaaca ggcccaacag gagcgacagg agcaacagga gcagatggag taacaggtcc
     7921 aacaggtcca acgggagcaa c
//
LOCUS       DACXMZ010000185         7783 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.186, whole genome shotgun sequence.
ACCESSION   DACXMZ010000185 DACXMZ010000000
VERSION     DACXMZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.186"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(58..885)
                     /locus_tag="JD016_15270"
     CDS             complement(58..885)
                     /locus_tag="JD016_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAU5000993.1"
                     /translation="MKIKDNKAVFHVFIRIFIIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(933..2369)
                     /locus_tag="JD016_15275"
     CDS             complement(933..2369)
                     /locus_tag="JD016_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HAU5000994.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(2456..3325)
                     /locus_tag="JD016_15280"
     CDS             complement(2456..3325)
                     /locus_tag="JD016_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5000995.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(3483..4136)
                     /gene="phoU"
                     /locus_tag="JD016_15285"
     CDS             complement(3483..4136)
                     /gene="phoU"
                     /locus_tag="JD016_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAU5000996.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(4164..4928)
                     /gene="pstB"
                     /locus_tag="JD016_15290"
     CDS             complement(4164..4928)
                     /gene="pstB"
                     /locus_tag="JD016_15290"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAU5000997.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(4947..5771)
                     /gene="pstA"
                     /locus_tag="JD016_15295"
     CDS             complement(4947..5771)
                     /gene="pstA"
                     /locus_tag="JD016_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAU5000998.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(5773..6690)
                     /gene="pstC"
                     /locus_tag="JD016_15300"
     CDS             complement(5773..6690)
                     /gene="pstC"
                     /locus_tag="JD016_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAU5000999.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(7071..7544)
                     /locus_tag="JD016_15305"
     CDS             complement(7071..7544)
                     /locus_tag="JD016_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001000.1"
                     /translation="MFVICISIYLAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQ
                     ILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGG
                     IGFKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIHTYCKHQVS"
BASE COUNT         2479 a         1443 c          826 g         3035 t
ORIGIN      
        1 tattatatgt tttattttat aaataattta ctttatcttt tactatattt aaatttatta
       61 taagttttct tgccagaaat ttttaggtgt tatattttct gtaattggca atattgtata
      121 tcctctctct tttaagattc ttataatatt atttaaagca tttaaacttt gctctttctc
      181 atgaattaat actactaatt catctctttt tcttccataa tataatatat tacttactat
      241 ctgacttgta gaagacttcc aatcctgtgt atccaaattc cagtcccaaa ttaaataatc
      301 atttgccata agtttattat atgaaccttc tggcatatat ggtttacttc cgtatggtaa
      361 tcttattaat tttgaactct gaccagttat ttcttgtaga gtattattgc atgtattaaa
      421 ttccccaact gttacttctg ggcttttata taacttatgt atatcatgac ttacactatg
      481 aaaacctatt gagttttctt cttccaagac tctccttact tcttccttgt ggtttttcat
      541 attctgatta agcatgaaaa atgtcgcttt gacatcattt tcttttaaaa tatcaagtag
      601 tgcatttgta tattttgatg gaccatcatc tattgttatg tatgctactt tttcagaagt
      661 agcccctgga ccttttgttt cttcttgaga tgctttgtat atgatttctc ctaaagtatt
      721 actttgagaa agcagttcca gtgtattttc accttttgaa cttatatttt caactggaac
      781 attattaatt ttttcctcca ataatgttcc agttaaaatt aaaacagcta taataaatat
      841 ccttataaat acatgaaaaa cagccttatt atctttaatt ttcatccccc atatctccct
      901 tttataaaag cataagttta agacttaaaa agttattttt tatatttatt ctctttctta
      961 ttacttttta tatctctact aaatattttt ttaacctttt cttcttcttt ttctcctaaa
     1021 gattctactc ttttgattcc caaagaagtt aaaaattgta atacatttaa attatcaaat
     1081 ggagtttcca tattcgtact ttcactatat tcatcaacat tcttagtacc ttcttccaca
     1141 cttaaaatac gcttcggacc tcctacacag cctcctacac accccattcc ttctataaat
     1201 gtagcttcta tttctttatt tagaactttc tttagaccgt ccatgcactc ttttgttcct
     1261 tcaaatgcct tttcttcaaa ttttatattt ttgtcaattc ttctaactga taacttaact
     1321 gctttactta cacctcctgt tctagcataa actctacctg aaagggacgc ctctactcta
     1381 ttactctcat ccattattgc tggatttaac tctaatgcat caaatatttc ttgcaactct
     1441 ttaaatgtaa gcacaaaatc aactgcatct tttatatctt ctaacattgc ttctttcttt
     1501 tttgccatac aaggacctat gaatacaact ttagcatctg gatttaaaat ttttacagct
     1561 ctcccacaag caatcatagg agaaacggat ggtgatacat tctccaatat ttgaggaaaa
     1621 ctattttgta tcaaactaac ccaaatagga cagcaacaac tagttataaa gcatccttct
     1681 tcgtcctttt tcatatgctc ataataatca tatgcttctt ttgcagtaag catatctgct
     1741 gccaaagcaa cttctatcat atcttcaaat cctatctttt ttaatgcagt tcttaattta
     1801 ccagggctta catctttccc aaactgtcct acaaaagctg gtgcaactat tgcatataca
     1861 gggctcttta tatcttttaa caattttatc attggtataa attctatttt atctgaaatt
     1921 gcacccaact tacatcttga tacacaatca ccacaagcac tacatacatc ttcctcatta
     1981 caattaatat gcttacttct tatcttacat gactcatctt ctttattatt ttcatcacaa
     2041 tatttgcaag catctgatat tacagatatt attggtcttt tgacttcatc taaatttaca
     2101 gcttcatgta gtgactcttt taaattttta tctgtatctt ctgtaacatc caatcccata
     2161 gttaccctaa ttaaatttac catagtatct ttatcaaatt ttccactaaa ataattattt
     2221 aaattatcct ttaaatcttc atttaaaatt ttattaggta agttttcaat atcctctata
     2281 tcatcattcc acactgcttt tacaagttcg ccaaaaagct tcattttata tatttctagt
     2341 attttagaat tgtcatttct agtattcata aaaaatagta cctccataaa ttaaacttat
     2401 atatgtttaa tatttacaaa gatactattt tttatttatt atttaactac tattattaat
     2461 atgatttatt tagctttctc aacaactcta tttcaatcat tattttcatt agatgatagt
     2521 tgaatacagg gcttttttct tcatctatat tccagtctag cttatccaca ttatatgttt
     2581 tcttaaggta tttatatgta tgctctttca aataaagttt attttcaagt aactctatag
     2641 actgcatatg aaaatgtatt tctctacata tccttaaggc attctcaatt ttttctatat
     2701 tagtttcact caaactataa tccaactcat caataagttt tgaatatatt tcttctaatt
     2761 tttctacttc ttcttcaaat ttctctacat ttaaattttc ttttctaaga attttatctt
     2821 caataagcaa cattgaaagt tcttctatct ttttaaactc atctataaca ctatttagat
     2881 aatttggtct agcaacataa taattaacaa gtactccaaa caaaacaccc acagatgtat
     2941 ctaacaccct atgtaaagaa taatacacag ggtcactatt tcctacacct agatgtatag
     3001 ccagaaaggt tacacaggca actactactg actttatttt aaacagattg cataaatata
     3061 ttgagcacat tactcctaga ccacataaaa atgggtctcc agctttaatg atggcaaata
     3121 tgaatcctat tataccacct aaaatagtac cttccactct attgattccc gtctttaaag
     3181 aaccttttac tgtatcttga acagaaatta tacatgctac cagagaataa aacatattct
     3241 ctactaaata gtcaccaaga atcatacata aagtgacact aattcctgtt tttatagtcc
     3301 tcatacccat cttttgaagt ttcaaagttc cacctcctaa aaagcacctt atataaaata
     3361 cgcatcctca tatttaatat gttcctagta tatcacaatt ttttaaattt acccattaat
     3421 catatttttt tatcaattct ttcttaattt agacaataaa aaaactgctt taaagcagtt
     3481 ttttatccta tctcaaccat tttaccctca attgcaaaaa ttatcttctc acatacattt
     3541 gttatatggt ctccaattct ttcaaggtat cttcctataa gtagaagttt taccccttgg
     3601 tctatggtat ttgagttttc atgcattatt tttaaacaat caatttgtac ttgttcatac
     3661 aatccatcta caatatcatc tttagttcca acatcataag ctatacttgc atcaaataag
     3721 attaaagctt ctctagtctg agacatcata gacttacatt cttttgccat cttaggaata
     3781 tctattagct cttttatata agcctctcca cctatcttca acatttctct agctatattt
     3841 gctgcataat ctcctattct ttccagctct atagatatat tacctaatga ataaataaat
     3901 cttaagtccc ttgccattgg ttgctttaat actattagct ctatactctt atctcttatt
     3961 tcttctctta atttatttat ttcttcatca tcttctacta cttttcttgc accctctaaa
     4021 tccttctcta tcatacaagt cacagacttt tctattgctt cttcacattt ttccatcata
     4081 agcactgttt gttcttttaa agtatcaata cttatatcta aactattatt gaccattgtc
     4141 taactccttt atagttatat attttaacca aatctacctg ttatatagtc ttctgttctt
     4201 ttatctacag gagtggtaaa catagttttt gtatcgctaa attctataac ctctccattt
     4261 aagaaaaaag ctgtttcatc tgatatacga gcagcttgtt gcatattatg tgtaactatg
     4321 actattgtgt agtctttttt taaatcctct ataagttcct ctacttttaa tgttgaaatt
     4381 gggtctaagg cagatgtagg ttcatccata agaataactt ctggtctcat agcaattgct
     4441 cttgctatac atatacgctg ttgttgacca ccagaaagtc ctagtgcaga tgatttaagc
     4501 ctgtctttaa cctcatccca aatagctgca ccttttaagc tttcttctac aattttatct
     4561 aattgcttct tatctcttag tccatgtgta cgaggaccat atgccacatt atcatatata
     4621 ctcataggaa atggatttgg cttttgaaat accattccta ctttagtacg aagatttata
     4681 acatcatctg aggtatatat gtcctctcca tcaacagaaa tgtttccttt aattgttaca
     4741 tcttcgatta agtcattcat tctatttaac gttcttataa aagttgattt cccgcatcca
     4801 gatggaccta ttaaagcagt aaccttattc tcttttatat ccatatttat tttctttaat
     4861 gcttgcttat caccataaaa taaatccaaa tcttttacac tcatttttat cttatcgatt
     4921 aattccatta ttcacacctc ttaaatttaa taattagctt tatttaattt cttagcaaca
     4981 aatcttgcta gcatgtttag tgctaataca attactatta aaacaattcc tatactggct
     5041 gctgttgcta tatctccact ctctttagtc agtaagtatg agtgtacagt taatgttctt
     5101 gcactatcaa atattccacc tggcatcttg gctacagtac ctgctgtaag taatattgca
     5161 gctgattctc caattatacg accaactgat aagattactc cagataaaat tcctggtatt
     5221 gcacttggaa gtactacctt atataaagtt tgaaattttg tagctcctaa tgccaatgat
     5281 gattctctat aagcttgagg aactgttttt aaagcttctt ctgttgtacg aattattact
     5341 ggtaatatta ttattgcaac tgtaagtgaa cctgcaacta tggaatatcc catttttaat
     5401 gtaactacaa agaaaattcc tccaaataat ccatatacta tagatggtat ccctgataaa
     5461 ctttcagttg caaatcttat agctgtaact gcttttcctt ttttagcata ttcttgtaaa
     5521 tatatagcag ccaatattcc tataggagtt gctacaacta tagaaactac tactgtatat
     5581 aatgttgtaa ttatcattgg aagtatacca ccatcacctg atgctgaata attgctaaat
     5641 aagaaattaa aattcatgtt gccaattccc tttataaata taaatcccac tataactact
     5701 aatgatacta ttgtaaatag tgctgataga tatactaatg acttcaatag attctctttg
     5761 aactttctca tcttaattta ccgccttatt tgaaagttta tttaaaacta agtttaaaat
     5821 taatataaat gtaaacaata ctactccagt tgcaaatagc atttcttgat gtgtaccaaa
     5881 tgcgtatccc atttcaagtg ctatattagt tgtaagtggt cttacactat ctgttattgc
     5941 agttggcata actggtgagt tgcctgcaac aagtattact gccattgttt cacctaaagc
     6001 tcttcctact cctaacacta ctgcggctaa tactccagat tttgcagcag gtaatacaac
     6061 tttgaatatt gtttctattt ttgatgcacc aagtgctaat gaaccttctt tgtatgcttt
     6121 tggtactgct cttattgctg tttctgatac agatattatt gttggtaaca tcattactgc
     6181 taatactatt actattgcta acaaactttg accttttgga agattaaata tactttgtat
     6241 agttggaact atcactgcaa gaccaaatac accatataat actgaaggta tacctgctaa
     6301 aagttcaact gctggtgaca ttatttttgc taatctttta ggtgctactt ctgctataaa
     6361 tacagaggtt aatataccaa caggtactcc tataacaagt gctccaagtg ttgcaaatat
     6421 tgatgctact atcatagatg atattccaaa tttattggca gaaggaaccc aatctgcacc
     6481 agttaaaaag tctataaatg aatatccttc aaatataaat ggtcttaatc ctttataaaa
     6541 tacgaatcct attatcaaaa gtaaacttgc aactgctact aaagcactaa gtagaaaaat
     6601 attttttgct actttttcaa ttacgtattt tgtcttattg ccattattgt catttttttc
     6661 taaacctact aatctagatt ttactatcat aataccctcc cattaaaata tacttatcga
     6721 ttttcgttct gtattcaaaa tttaattttt tataattcgt cttcatcaaa tttcctatac
     6781 ttttattcta ctttttttaa gttaaatcta tattaggatt aagttaagca agtgtaaaat
     6841 tatatttact ttttaacatt aattttactt ttaaaactat atttgacatt tactttacaa
     6901 tatactgttg tttctctata ttaaaatagc agatatagat aataatacag tttatgtata
     6961 atttattacc tatatctgct attaagattt gttaactaaa tttaactttt atactacata
     7021 tataattatt gtatcctcta gtttatatca ctattatttc catcaaattg ttacgatact
     7081 tgatgtttgc aataggtatg aatagccaaa aatataaaaa taggtatttc tatcactgag
     7141 aaaaacagta taatgtgtgg tgtccaaaaa ctcatacctc ctaagtgtaa gaaattaaat
     7201 gatgtcattt gatataataa actaatctta aaaccaattc cccctggcgg taataggtat
     7261 ccaatccaac taacacctat tcccatagga attaagcaaa tagaaaaagc aatcagcata
     7321 gaagtaagag aatccttaca ttttgcagat aaaaataatg ttaaactaac cattgcaagt
     7381 attgaaatta gaccaccaat agctataaga atctgtgctt gtagtaaatt tatatttact
     7441 agactaatga cagaaaacaa catttgaaat gaggttttca aacattcagt accaaaagct
     7501 aaatcagaaa ttgccaaata gatagaaata catataacaa acattattat aaatatagaa
     7561 aatagagcta atattttagt aaacgctaga caaatatgtc catgtttcgt acaacgaagg
     7621 atgctatctg attgtgtctg atactcacca gaaaaagtag gtgcagcaat tgcaacatat
     7681 ataattaata atataaaaat ataaaatatt atataatcga aagcatcttc tggatttatt
     7741 aagaaattct taatcttatt atttaactat tttattattt aat
//
LOCUS       DACXMZ010000186         7749 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.187, whole genome shotgun sequence.
ACCESSION   DACXMZ010000186 DACXMZ010000000
VERSION     DACXMZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.187"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            67..378
                     /locus_tag="JD016_15310"
     CDS             67..378
                     /locus_tag="JD016_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAU5001001.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      471..563
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            663..1457
                     /gene="thiD"
                     /locus_tag="JD016_15315"
     CDS             663..1457
                     /gene="thiD"
                     /locus_tag="JD016_15315"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAU5001002.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1481..2269
                     /gene="thiM"
                     /locus_tag="JD016_15320"
     CDS             1481..2269
                     /gene="thiM"
                     /locus_tag="JD016_15320"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAU5001003.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2292..2924
                     /gene="thiE"
                     /locus_tag="JD016_15325"
     CDS             2292..2924
                     /gene="thiE"
                     /locus_tag="JD016_15325"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAU5001004.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3063..3365
                     /locus_tag="JD016_15330"
     CDS             3063..3365
                     /locus_tag="JD016_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAU5001005.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3431..4186)
                     /locus_tag="JD016_15335"
     CDS             complement(3431..4186)
                     /locus_tag="JD016_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5001006.1"
                     /translation="MGMLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMN
                     NININNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQL
                     LEAILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLP
                     NEIVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETI
                     LIGDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4174..5136)
                     /locus_tag="JD016_15340"
     CDS             complement(4174..5136)
                     /locus_tag="JD016_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001007.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5140..5598)
                     /locus_tag="JD016_15345"
     CDS             complement(5140..5598)
                     /locus_tag="JD016_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HAU5001008.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKVQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5954..6310
                     /locus_tag="JD016_15350"
     CDS             5954..6310
                     /locus_tag="JD016_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU5001009.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6382..7098
                     /locus_tag="JD016_15355"
     CDS             6382..7098
                     /locus_tag="JD016_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001010.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7091..>7749
                     /locus_tag="JD016_15360"
     CDS             7091..>7749
                     /locus_tag="JD016_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5001011.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYVFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     L"
BASE COUNT         3007 a          905 c         1263 g         2574 t
ORIGIN      
        1 tacacaccat tttttttaat tctataatat aatatttata tatttaatgt taggatgtga
       61 tttaaggtgt attttacata tattataaga tgtaaagatg attctctata cactggatat
      121 accagtaata tagttagaag aatgaatgaa cataaattag gaattaattc aaaatacaca
      181 agagcaaagg gatttgaaaa actagaagtt tattttgtga ccaacacaaa aagcaatgct
      241 atgaagttag aatattatat aaaaaaatta actagaaata aaaaattgtc tataataaaa
      301 aacccaagta tacttataaa tttaattgat aataaagaag attatattat tggtaaggaa
      361 atagaacaat taacataaat atttatagta acattataat gggtgcatat ggtgtaaaaa
      421 cttgacaata aatactattg taatataatg aagttataaa tatagtaaat taaaatagct
      481 aggggagcca agagctgaga ggtataccga cccttacacc tgatctggat aataccagcg
      541 taggaaagct tagtaaaaat tcaagtatgt attatttgtg atatatgcta tttctaagct
      601 atacctattg taggtatagc ttttttaatt aagttattta aattaaactt taggaggcaa
      661 caatgagtaa ttataaaata ccaacactta caatagccgg ctctgattca tctggaggag
      721 ctgggattca ggctgatttg aaaacgttta gtgcaattgg tacttatggt atgagtgtaa
      781 taacagctat aacagcacag aatactaaag gagtttttgc tgtagaggat ttaaataaga
      841 aaataataaa aaaacaaata gaggcagtct ttgaagatat acctccaaga gctgtaaaaa
      901 taggaatggt ttcaagtcct gagattatac ttgaaatagt tgagaatcta aagaaataca
      961 atccaaaata tttggttgtg gacccagtaa tgatatctaa aagtggatat tatctattaa
     1021 agccagaggc aaaagaaaac ttaattaagt atttgatacc acttgcatac ataattacac
     1081 caaatatacc agaggcagaa gaaataacag gaataaaaat acataatgta gatgatatga
     1141 agagagtagg agaagaaata ctacaacttg gacctaaatt tgtacttatg aaagggggtc
     1201 atttagatgg agaagcagtg gatattttag ttggtaaaaa tatatttaaa gtttataaaa
     1261 gtgaaagaat tgataaaaaa aatacacatg gaacagggtg cacattatct tctgcaataa
     1321 catcttatct agcattagga tatgaaataa cagaagcagt aaatctaagt aaaatctata
     1381 taacagaagc aataaaaaga agttttgata taggtcatgg agtaggacca gtacatcatt
     1441 tttataaatt tgagtaataa aaaaatattg gaggtagaat atgtataatt taattaaaga
     1501 tgtaaaaaaa ttaaatccat tggtaattca ttatactaat aatgtaacta taaatgattg
     1561 tgctaatgta actttagctg taggagcaag tcctcttatg agtttttcat atgaggaagt
     1621 agaagaaatg gtaagtgtag caaactctgt agtaataaat ataggaacta tgaactcaaa
     1681 tatgttggat ttattcctat tagctggaaa agcagcaaat aagtacaata aacctgttgt
     1741 tttggaccca gttggagtat ttgcaagcaa agcgagagca gaacttacaa gtagactttt
     1801 aaatgaagtc aaatttagtg ttgttaaagg gaatgtatca gaaataaagt ttataggtgg
     1861 ctttaatgta aggggtaaag gtgtagattc atttgatgaa gaagaagatt ctactgagat
     1921 aataagaaag attgctgaaa agcttgaatg tgtagttgta gcaactggaa aaatagatat
     1981 aataactaat ggtaaaggta catataaaat aaacaatgga actgataaat taaaaggaat
     2041 tacaggaact ggatgtatga ctgctagctt gattgcaagt tttatggctg ttactgagaa
     2101 tatattagaa gcagcaacaa tgggtgttct tacaatgtct ttaagtgggg aattagctaa
     2161 tttaaataat ccaccaatag gtacatttaa agaaaatctt atgaatgcta tttatcaaat
     2221 ggatatagat actttaagta aaaattctaa tattgaattt ttaaattaat aaagagtaag
     2281 ggagacttaa aatgattgat aaagaatctt taaaaaaatg tttaaagttg tatttagtaa
     2341 ctgactctga aatgcttaaa ggtagagact tttataaatg tcttgaagat gcaatatctt
     2401 ctggaataac aactgtacaa cttagagaaa aaaatgcttc tggaagagag tttttaagaa
     2461 aagctatgaa acttagagaa ataactaaac gatatggtgt taagtttata ataaatgata
     2521 gagtggatat agcacttatt tgtgatgcag atggagtaca tgtaggacag agtgacattg
     2581 atgttagaga ggttagaaag cttataggaa acaataaaat tctaggagta tcagcaagaa
     2641 ctttagaaga agctatttgt gcaaaaaatg atggcgcaga ctatttaggt gtagggtcta
     2701 tattcacaac atctactaaa ttagatgcaa aatcagcttc gtttgaaact gttaaagaaa
     2761 ttaaagaaaa agtagacatg ccatttgtac taattggagg tataaattta gacaatatag
     2821 ataaacttaa atgtttagaa agtgacgggt atgctataat atcagctata ttaaaagctg
     2881 aagatatctc taaagaagtg gaaaagtgga cattaaaaat ataaaattgg ctctggtagt
     2941 gaaaaaaatg gaaaaatttg atataatcta agagtaaaat ttaagcaatt ataaatgata
     3001 aacttttcta catatacttt ttaatcaaga tatatgtaga tttggttaaa aaggagctat
     3061 aaatgattag gatattgtta gtgtgtgttg gaggcatgtc ttctacccta ttggtaaata
     3121 aaatggagaa ggatgcaaaa aaaagaaata tagactgtaa aatatgggca gttggagaag
     3181 gagatataaa atcggagctt gataactttg atattttact tcttggacca caacttagat
     3241 ttatgcttga tgacgtaaag tctatagttg gagatagagc tccagtatct ataatagata
     3301 tggtaaacta tggcacttgt aatggtcatg ctgtgttaaa ttcggtttta gaaatattaa
     3361 aataaataat aaaagtggat tctatataat agagtccatt tttattattt gttattaata
     3421 ggcttaaaac ttagcttacc atttcttttt ttaatctcca catagcaagt gaaaaacttg
     3481 aacagcacaa tattaaaaat agtaggataa caggcagtat atctccaata agaatagtct
     3541 caccaaatat taagctacgt aaagcgttaa taacatgggt aaatggattg agcatcactg
     3601 ctacttttaa tcctccagat aaactttcta tagggaaaag agctgaactt aaaaagaaaa
     3661 taggaagtac aattgcagtc ataattgttt cataaactat ttcatttggc aataataaac
     3721 ttatagaata agctagactt gataaaaaga aggctgtcat aaatatcagt actatcataa
     3781 gtaggattcc accaatgcca gattctatcc ttactgagaa gaatatactt actatacaca
     3841 aaatagttac ttctatgaaa gaaactaaga tagcttctaa taattgacca aggacgattg
     3901 aatacctact gataggtgca attaagactc tatagaaact tccactgttc ttcattataa
     3961 agtttataat accaccacta ctacaagaag aaaacacaac cagtacaatg atacctggta
     4021 gtataaaagc agtataatta ttgatattta tattattcat agtttggttt gcaatagaac
     4081 tataaagcac aagccaaaga aaaggttgta atatagtaac tacaaatgag attggatttt
     4141 taaaacgcca cttcatacta cgccataaga tatttaacat tcccattcct cctttaagct
     4201 ttttttagaa cttgtaagtg ctaaaaatac atcttcgaga cttggctcta caatttctat
     4261 tgcatcaaat tctatatcat gttctaaaag ccatttattt attaaagtaa aagcagttct
     4321 actatcgttt acacttataa aaattgaatt ttctcgtacg ctcataaact ttactaaacc
     4381 agtactttta attgaatctt tatatttttt tatatcttca gtattatgaa aagttatacg
     4441 tagtatattt tgtctaatgt aactacgtaa acttgatggt gttccttgtg ctaaatcctt
     4501 tccattcttc ataatacaaa tattatcact caattgttca gcttcttcaa gataatgagt
     4561 agttagaaat atagttgtgc catactcatc tctaattttt aataacatat cccacataga
     4621 ttttcttgaa tcgacatcca taccaacagt tggttcatct aaaaatagga ttttaggaga
     4681 tgataccata ttcatagcta tgtccaatct acgttttaca ccccctgaat atgatgaagt
     4741 aggatatttt aagtaactag ataagtcaaa cttatcaatt aaagagtcta ttctttgttt
     4801 tgcaatctgt ggttctactt tatacatttt actttgaaat accatatttt ccataaggga
     4861 taaatgttca tcaatagaga tttgctgtgc tacacaagca atttgagtac gtatccaaga
     4921 aggattatct actaaatctt ttccaaacat tgttacattt ccagatgtag gtttgtaaaa
     4981 ggtagtcaaa atatttataa gagaagattt accagcacca ttaggaccaa gtaaagaaaa
     5041 aatttctcca gcatttacat taaagcttag accatttaaa gctttcacac catttttata
     5101 ttcttttact aaattgtcta tttcaatcgc taacatatat tattcctcct ctttaagtgg
     5161 aaatattatc tctgtaatat agttatctgg gtttcctttt atcaacatac ctggtccttt
     5221 aataaatact tctctccaag gagtttgtac ttttatatta ttttcatgaa tatatttaaa
     5281 tatcatttca tacgtttttg gcaaattact ataagagcct gtgtgagtaa aaaataaagc
     5341 ttttgtttta ggaaattctt ttgtagttat acccatacga tttggagttt cagcagttgg
     5401 aacacataat tccatttctg caacaccagt tttttggtca acatcatagt agcaaaagaa
     5461 tggtgcaccg ttagactttc cttttatagc tttaaataca tttggaaaat actttgttgc
     5521 ctcagtcata ggtccattat aacgcatggt agccactgtg actgattcga tatttttgat
     5581 ttcaattttg caattcattt gtttacctcc taatgtgtgt tcatattctt actagatttt
     5641 taagcattaa tacatataag attttgatta gttgaaaaca tataatctta tctcgtttgc
     5701 ttgttttcaa gtctaacata acataaaata tatatcctct catttgatgc tccattttgt
     5761 aaagttatta tttaagcttt actttagatt ttttagcaaa ttaaaataaa gcaatttata
     5821 aaataagtat ttacaaatta agtgtattaa tcgtataata cacttaatag gatactagat
     5881 aaagtctgaa ataaaggcgt atttttttaa agtaagtgta ctatcgtatt aatacactag
     5941 aataggagga atcatgaatt ttgaattaga taatacaact cccatttatt tgcaaatagt
     6001 aaagtatata aagagacaaa tagtaacagg agagttgaaa ccaggagaga caataccatc
     6061 aagaagagaa atggcactta atctaaaagt aaacttaaat acagttcaaa gagcatataa
     6121 ggagatgggt gatatgaata taataaatac ttttaaaaat tatcaaagca gtgtaacagt
     6181 tgatgaaaat atattaaaaa atttaaaatt agagcttata aatgaatcac tatctgtttt
     6241 tatagaagat atgaaagcaa taaatgttag caaagaagaa gttttaaaaa taatagaaga
     6301 taagtattag ttttttaaca aatagaaaac ttagtttaga aagtttattt ataagcatat
     6361 tggatgtgga ggatagtaaa tatgatagaa ataaagaatg tcagcaaaac atataaaagg
     6421 atgcaagggc ttaaaataag aaagatagaa gctttaaaaa atgtaagttt taatatagaa
     6481 aagggaaaaa taactgcatt acttggtata aatggtgttg gtaaatcaac tatgttaaaa
     6541 gcaatagcag gacttataaa cattgatagt ggagaaattc gtatagatgg agaaaaaata
     6601 aatgaaaaag tatacaataa attggctttt gttccagatg tacaatctca ctttagcaat
     6661 acaacaataa aagaaacatt tgaatttatg gaaatttttt attcaaagtg gaataaagaa
     6721 aaatcaaaag aaatgatgga catatttaaa ttggatgaag atgaaataat tgataacttg
     6781 tcaaaaggaa acattgcaag agtaaaatta atacttggat tttgccaaga cccagagtac
     6841 atacttcttg atgaaccatt tacaggaatt gatttgttta agagggaaga gtttatagga
     6901 gtaatagcgc aatatatgga ggaaaatcaa gccattatca taactactca tgagatagta
     6961 gagatagaaa gtttagttga tgaggttgta attcttgatg aagggcagat tataacttca
     7021 tttaatgcag aagaattaag agaaagagaa ggtaaatcta tattagataa gatgagggag
     7081 gtatataaga atgaataaac ttctatatct aataaatgta gatttgagaa gaagcaataa
     7141 gttttatata gcttatatat catttttttc attaattaca cttagtttaa atttattcga
     7201 aattaataaa tttaaaaatt ctaaatttat tatgaactta gcaatagaag attttggagg
     7261 tatattttat ggactaggga tacttagtaa tcttggtttt atccaaagta tattgttctt
     7321 tgggatattg ggacttttta tatactatgt ttttatgtgg aaaagagagt tcttttcaaa
     7381 taatcaaagt atttatacac ttatgatgtt acctcaaaat aagtttaaga tttgtatatc
     7441 taaatcaata gccttgataa caatgatata tggttttttg ataagtcaga cagcaacatt
     7501 atttatatgt aaatatatat ttaatttcat ttttagaaat atgcctataa tcaatatgaa
     7561 ctttgccaaa gatttatatt atttaaactt gaagagtata ccaattgatt ttatctcttt
     7621 tatggctatt tatgtatttt tactacttat agctatttct attgtaaatt gctgtatatt
     7681 atttatcttt tcctatataa ataaatttta tgtaatttta atcactttgc tgattatagc
     7741 aggcttata
//
LOCUS       DACXMZ010000187         7747 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.188, whole genome shotgun sequence.
ACCESSION   DACXMZ010000187 DACXMZ010000000
VERSION     DACXMZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.188"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(224..2503)
                     /locus_tag="JD016_15365"
     CDS             complement(224..2503)
                     /locus_tag="JD016_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAU5001012.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2774..4228)
                     /locus_tag="JD016_15370"
     CDS             complement(2774..4228)
                     /locus_tag="JD016_15370"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAU5001013.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4392..5261
                     /locus_tag="JD016_15375"
     CDS             4392..5261
                     /locus_tag="JD016_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAU5001014.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5474..7546)
                     /locus_tag="JD016_15380"
     CDS             complement(5474..7546)
                     /locus_tag="JD016_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAU5001015.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PVLANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNDLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT         2628 a         1294 c          822 g         3003 t
ORIGIN      
        1 ttactatcta aagtattttg ctataatccc atatactctt acctcaaaaa cattcaaatg
       61 taaaaaatac cacatattaa taaactttgc ccaataattc ctaaaataat atcctaacaa
      121 tgatttatgt ttttataata gtatattttt acataaaaaa gtgaacattg attaacaaag
      181 ttcacttttt tataaatttt tatgttataa ttcacaattt tatttaattt gtttcttttc
      241 tatttaaact atatctatct aatattaatt tttcatattc ttctattggc attggtctag
      301 caaataaaaa tccttgagcc atatcacaac ctataaacct taaaaactct gcttgctcta
      361 cagtttcaac accttctgaa acaactttta tatcaagttg ctttgccatt tgaacaatac
      421 ttgaaactac aatttgactc ttttttgcat ttcccactgt tataaagaat tgcctatcta
      481 atttcaatac atcaattggc atatctttaa gcatatttaa agaagaataa ccagaaccaa
      541 aatcatccat agaaatttta aatccaacag atttcaaagt ttgcattatt ttaaataata
      601 tttttgtatt gtcaaatatt gcactttctg tcaattcaat ctcaattaaa tttggttcaa
      661 tttcatattc ttttgttatt ctttcaagct cagaaataaa gcctggattc tcaagatgta
      721 ctcttgataa gtttattgat ataggaaatg tggggaaatt ttcattttcc caacgtttaa
      781 aatttttaca aacttgggta aatacaaaca tatccaattc tgttataaaa ccatttttct
      841 caaaaatagg aataaataca cctggactta ttagaccttt tttaggatgt tgccaacgta
      901 caagtgcttc agcacctaca attttagtag ttgataaatc aaccttaggc tgtaaataaa
      961 ctttaaattg ttcatttttt aaagcatcat gcatttcttg ttcaatatca cgttcttcta
     1021 tgagagtttc ttttaaattt tcagcataaa tctcataaac ttttaaatat tttcttttta
     1081 ctgtagtttt tgcaatcata gctttgtcaa gacaggtaga aatatttgtt tctccctctt
     1141 caacaaaata tacacctatg gatggaatta aattgagttc tactcccaaa cttgttttta
     1201 tcattgacaa ttttttacgt ataagttcac atatttctat aattgactct ctattatttt
     1261 tcttttcaaa tattatagca aaattgtcat ttgaaattct tgaaaataca gattgctctt
     1321 taaatatatt cttaattatc tctgctattt cctttaatac tctatctcct tcttcatacc
     1381 caaacctatc atttactagc ttaaacttat ctatatcaaa ataaattaaa actagattct
     1441 ttttctcatg agaggataaa aattttcccc cttcaagatt aaatttattt gaattgccaa
     1501 tacctgtaac gttatcttta aaagcaaatt cttccaattt ttccttactc tttttatgta
     1561 taaatagtat ataaattgtt aatgccgtaa agattataat caagactaca catgctgata
     1621 atgtaagttt tataatagca tatgatttct cagatatagc agttttagga acttcagaaa
     1681 aaagatacca atcattgatt cctattgggg cataacttaa ataatatcct tttccaaaca
     1741 tactataact taatgaacct gatttaaagt tttttatatt tgacttcata ttattgatac
     1801 tagattttga tattgaatca cttctgccaa tgtaagaaaa taaattttct ttagtagagt
     1861 tggcgttttt actgcttgga tttaaaacaa tatcaccatt acttctagtt atataagaat
     1921 atccttttcc attaaatgta ggtacagaaa gtattttttc atacaacttc gtactatgtg
     1981 tggcaaatat aactccttta acctcattat ctttataaat tggtacagaa tatacattaa
     2041 tattactatt ggtattttta tcatcttcta ttacatcttt taattttcct gttataacca
     2101 cttctccttg cattgatttt aagaaataat ctcttttaga gaagtcttct atataaccat
     2161 ctgtagtgta tgctgtacca tttggtaaaa taactcccat tcttttaaat gaactattaa
     2221 tagcttgtct ttttagtata gaaaggatat tatctacttc aaaagactct tctccttcaa
     2281 taaatattga aattgactct agtaacttta tatctccatt aatttgtttt tttaacacat
     2341 tgacgctttg atttgctatt tctgaaagat attgattcgt ttcttcacct aatgtcttat
     2401 tcaaccttga accataaatc aaacaggaaa ttgtaattac taagatactt atgacagcaa
     2461 ttatggacaa accataccta ttattttttg catcacttaa cattatgctt cctgcctttc
     2521 tcgaatctat gtatgtataa tcattatctt atgacaatta ttgtaaacat tttataataa
     2581 tacttatttt tccatattta ctgtaattat acaaaattat aaactttatt acaacaataa
     2641 attaattaat atagatatta gtgtttatat tattgcatta tctattttac aattatttac
     2701 atataaaaaa atgtaatatc aaagtgaagc ttgatattac atttttgttc ttgtattttg
     2761 tttaattttt atgctacatc ttacttaaac actcatttgc aacttcttta tcatcaaaat
     2821 gatgcttaac tttacctata atttgataag tttcatgtcc cttacctgtt ataattacaa
     2881 catcatcctt ttttgccata cttatagctt ctgctatagc ttgttctcta tcaacaacaa
     2941 catggtaatt ttcttttttc ttgtcaattc cttcaagtat atcttttatt attgcctctg
     3001 gttcttcagt tcttggatta tcagaagtta taatacataa atcagaatat ttttgtccaa
     3061 ttgctcccat caaaggtctc ttttcagtat ctctatctcc accacaccca aacacagaaa
     3121 ttatttttcc ttctgcaaat tgttgagttg tctttagtac attttcaagt gcgtcaggag
     3181 tatgagcata gtctacaatt acgcttatac ctttatcatt tggaactgtt tcaaaccttc
     3241 ctgatactcc attagaaagt ctaaggcctt ctttatatat tggtttaggt atgccaagta
     3301 catagcaagc agctataact gctaaagtat tatatactgt aaacattcct ggaactggta
     3361 taaatattac ttcttcatat gaaggtgtta ttaatctata agatactcca tccgaatctg
     3421 attttatatc tctagccata aaatcagcct tagtatcaac accataagtg taacaaggta
     3481 catttatacc ttttatattt tcatatatct tctttccacc ttcatcatct atgtttatta
     3541 tatttgccat agtagtctta aaaaataatt tttcttttgc ttttctataa tcctctaaat
     3601 ctttatgaaa atctaaatgg tctggtgtta aatttgtaaa tatgcctagt ttaaagtctg
     3661 tttcatcaac tctatttaat gccaatgaat gtgatgaaac ttccattgca cagtagtcac
     3721 atccattatc caacatatta ttaaaatgat attgtaaatc tatactttct ggagttgttg
     3781 aattggaaac aatgtctttt tctccatcaa aaatttttat agttcctata agtccaactt
     3841 tattcttatt taaagttaat atctcattta gtatagtagt tatactagtt tttccattag
     3901 tacctgtaac tcctataacg ttaaattttt gagatggatg attgtaaaaa ttatcagcta
     3961 cttttgccat atcttttctt gtatctttta cctttataaa tgtacatcct tttattgcta
     4021 catcttcttc aacaagaaat gcacttgctc ccttttctat ggcatctttt atatacttat
     4081 gaccatctga cacaaatcct tttatacata taaataatgt accttttgta acctttcttg
     4141 aatcatattg tacattatta atatctatat ttaattcacc cttgacattg attatatcca
     4201 acccctgtat aatatcattt aatttcataa atgctttcct ccctttaaac tctttctgct
     4261 aaaattacac tacatataat attatattaa gaatttttta ctatgtgaat ggttaaaaat
     4321 gcttgaaaca ttgcaaaaaa tgctctataa ttttctataa tataactgag gaaaggtctt
     4381 ctataaaaac tatggataat aaaatcgggt acttaaacga taactttaaa atatttaata
     4441 ttagagataa aaaagatata caatttgaat accataatca tgattttaat aagataatta
     4501 tatttataaa tggaaatgta acttattttg ttgatggtat tccctataaa ctaaagcctt
     4561 gggatatact ctttatcagc aataatgaaa tccataaacc agttatagat tcaaatgtct
     4621 tttatgagag aattgcaata tggattagcc cagaatttat gaaaaaaaat agtgatgcta
     4681 gtagtgactt agaaaaatgt tttaaaatag ctacagagaa taaatgcaat agattgcgtt
     4741 taaatatgca tgatattgaa tctattaaga gttttattga gcaaataaat atttccgaat
     4801 ccaatactga atttggaaat gaaatattga gaaatacatt atttatacaa cttatgatat
     4861 tcttaaacag aattttccta aaaaatgaaa atatggaatc aacagaagat gttttttatg
     4921 atgaaactat taagaaagta ttaaactata taaattcaaa tatagatgat aagctttcaa
     4981 tagatagcat atcgtctgaa ttttttataa gtaagtatta tcttatgaga aaatttaaat
     5041 ctcatactgg aacgtctata cacaattata tagttcaaaa aagattaatt ttggcaaagt
     5101 cactaataac caatggtcac cttatgagtg aagtttgtaa taaatgtgga ttcaacgatt
     5161 attctagttt tgtaagggca tttaaaaaaa actacggggt atctcctaaa aattatataa
     5221 acaataaaaa tcctttggat attactcttt tagatgaata gataatttat gtaaatttta
     5281 ttttaataaa acttgaagta ttagtgtatt tatcttacta atacctcaag ttttttaatg
     5341 ttttaaaata tatcaaaata taatgatata tatttctatt acttaaaata tatattaaat
     5401 aaattgcatg ttaactgaac ctatttatat atttattaaa tcccacataa cttttagtta
     5461 cactatcata tagctatcct attttttcaa tatcatctct taatatactt actctattct
     5521 cctcttttgc tgtggttact ccttttttaa tgcttaattt ctgattttgt tctaaattcg
     5581 taaatactac aggacttatt gttgatagat catttttttc aatatattct aagtccattt
     5641 taactaattt ttctcccgct tttacttcat ctcctacatt cacaaatact tcaaatccat
     5701 ttccttctaa atttacagtt tctatcccta aatgtataag tatttctact ccactttttt
     5761 tggattttat tattattgca tgcttagttg gaaatataac tccaatagtt ccatcaactg
     5821 gtgaataaat caatccatca ctagatttaa ttgcaaatcc atcccccatc agtttacttg
     5881 aaaatacaga atctggcact tcttctatat ctaatatctc tccatttgtt aatgaatcaa
     5941 tcttttctac taaagcattt tccatatttg caacttccat attttttttc actttatttt
     6001 tatcaacatt tttagtaaca gtactttcaa cctttgtttt tccctctatt atatctttta
     6061 tatcagactt tattgaatcc gattttggtc caaatatagc ttgaacattg ttgccaataa
     6121 ccataacacc tgctgctcca agtgatttca atttttcttt atctacttta tttatatcat
     6181 ttacaaccaa tctaagtctt gttatacacg catctaggct ctttaaattg tctttatttc
     6241 ctaaagcaat caaaatctct gatgctaatt cattcttaga catttcaatg acttcagcgt
     6301 tagtttctga ctcatcttct cttcctggtg tctttaaatt aaattttctt atcataaatc
     6361 taaatacact atagtataat actgcaaaac caatacctac caagatagcc aagtaccatg
     6421 gagttctatt aggaagaaca ccaaataata agaaatctat taatcctcct gaaaatgtga
     6481 atccaatttt aactcctaat aattgcataa ttgcgtatga taaacctgaa aataaacaat
     6541 gtattgcaaa taatactggt gcaacaaata aaaacgcaaa ctctattggc tcggttattc
     6601 ctgttaaaaa agaggttaat gctgctgatg ccattatacc tgctacaaat tttttctttt
     6661 ctggctttgc ttcatgcacc atagcaagag ctgctgctgg aagaccaaac atcataaatg
     6721 gaaatgcacc tgtcataaaa gtacctgctg tgaaatttac tccatcttta agttgagcaa
     6781 agaatatctt ttggtcaccc attacaagct caccagcttt atttacatat tctccaaatt
     6841 gataccaaaa tggattatgg aaaacatgat gcaaaccaaa tggtattaat agtctttcca
     6901 aagaaccttc cataaatgct gctagtgttt gatttgcttc tataacattt tttgagaaac
     6961 taagtaatcc ctcttgtatt ggaggccaaa taaaagacaa tataattcct attgctatag
     7021 caactaatga agtaacgatt ggtacaaatc ttttacctgc aaaaaaacct agatactgag
     7081 gtaattcaat tttataaaac tttttataaa gataagcagc cactatccct ataataatac
     7141 caccaaatac ccctgtttga attgttggta ttccaacaat atttgcatac atagaattgt
     7201 ctaccatatc ttctgtaaca cccgccataa cacccataga tgtattgaga attaaaaaac
     7261 ctactatggc tgccaatgct gcaacacctt ctccttcagt taaaccaaca gctaccccaa
     7321 ctgcaaatat aagtggtaag ttattaaata taatttgacc tgaactttcc attatactta
     7381 ttataagttg aaatgtagga ttcgccaaaa ctggaaaatg agccaacaat gcgtctcctg
     7441 ctaaagcatt actcacgcca agtaatatac ctgctgcagg aagtaatgca actggtagca
     7501 ttagagattt tcctactttt tgtaaaacac caaatacttt tttcatttac ttttctctcc
     7561 ttattatttt atattttttt aaataaaaaa gacttaagtt tataaaaagt tacattagta
     7621 tacttaattc taaaattaaa cttatgttaa tataaacaaa cctatattaa cctaatttca
     7681 gaattaaaaa taaactatta taattttttt ataaacataa gtcttgcctg cattatcagt
     7741 aacaatc
//
LOCUS       DACXMZ010000188         7689 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.189, whole genome shotgun sequence.
ACCESSION   DACXMZ010000188 DACXMZ010000000
VERSION     DACXMZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.189"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            199..720
                     /locus_tag="JD016_15385"
     CDS             199..720
                     /locus_tag="JD016_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU5001016.1"
                     /translation="MDIAKLILNPARLRILQYIRLHGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKRDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            754..1530
                     /locus_tag="JD016_15390"
     CDS             754..1530
                     /locus_tag="JD016_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAU5001017.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSANM
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1872..2825
                     /locus_tag="JD016_15395"
     CDS             1872..2825
                     /locus_tag="JD016_15395"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HAU5001018.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2843..3862
                     /locus_tag="JD016_15400"
     CDS             2843..3862
                     /locus_tag="JD016_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HAU5001019.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3893..4144
                     /locus_tag="JD016_15405"
     CDS             3893..4144
                     /locus_tag="JD016_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001020.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4426..6231
                     /gene="lepA"
                     /locus_tag="JD016_15410"
     CDS             4426..6231
                     /gene="lepA"
                     /locus_tag="JD016_15410"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAU5001021.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6371..>7689
                     /locus_tag="JD016_15415"
     CDS             6371..>7689
                     /locus_tag="JD016_15415"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAU5001022.1"
                     /translation="MIFMINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPN
                     DWDITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDS
                     IEFTNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDA
                     LRMLRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLL
                     KSTGLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGK
                     KVTKTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRK
                     IIKKLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECF
                     NLKDLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILK"
BASE COUNT         3074 a          868 c         1383 g         2364 t
ORIGIN      
        1 tttcattagt atgtatgata gatgcatcaa acaatataac taatatgtta gaaggttata
       61 tgtaattaat gtcagtgttt atatagaata gcatttgtag catttattat agttgtaatt
      121 actatttata tcatagactt gaatagtcta aaaaaataga ctataatatt tttatagact
      181 aaagaaaggg ctgataagat ggacatagca aaactaattt taaatcctgc aaggcttcgt
      241 attttacaat atattcgact tcatggcagt gttcgtacat ctgatattgt aaagtattta
      301 aatgatattc ctcgtgcaac agtttatcac catgtaaaaa ttttagaaga gaacaatatg
      361 attgaagtaa ttaaagaaaa tcgtgtgcgt ggtactattg aaaaggttta tgcactaaag
      421 gaatatacaa catctatgga aggagagacc ttcgttgcac tttctacagc attttatgta
      481 ggtctgatgc aggaaatgaa tgaatacttt agcagaaaag agcaagacca taaaagggat
      541 aatgtatttt tctcttcagc attactatat atgagtgata atgaatatga aaatcttctt
      601 aaaagtatag ctgatttatt gaaaccatat atagaacaaa aacctaaatc aaatctaaaa
      661 cttagaaaac tttcaattat atcttctcca cctgtagctg ataatgataa aacaaaataa
      721 catgcaaccc atttttataa aaggaggtta tatatgaaat ttcatgagtt tggaaataaa
      781 aacagaccac atattatgtt gattcatggg ggagggaatg catggtggaa ctatttgcga
      841 caagcagaag ttttgtctaa caaatatcat gtgatacttc ctacattgga tggtcatgga
      901 gaagaatatg aaacaacata catttctaca gaggatacag cagataagtt gatatcatat
      961 atagatgaaa attgcagtgg acatcttttc gctttgtgtg gggtatcact tggtggtcaa
     1021 attgtcatgg aacttttatc tcgaaaacct aatattacta aaaaagcaat tattgatggt
     1081 agtatttgct acccaagacc tcttatggca aggttttgta ttgctggtat tcgatttttt
     1141 ggaggacttt tgtttagtga gaaggcttgt cgctttcaaa ttgctatgat gccaaaattg
     1201 ttacctgaaa atatgcaata tccagatgaa ataaaagcat attatatgac agatatgcca
     1261 catgtaagaa aagaaacatt ctataatatg tatcgcactt acatgatgaa ctatacactt
     1321 aaagagaatg ttaaaaaaac cacagcacaa gtaatgtact ggtatggaga aaaagaaatg
     1381 aaatgtgtta aaaaatctgc aaacatgttt cagtcttatg tgccctcttg tgagatttat
     1441 gaagctaaga gctataatca tgggtattta tcactttatc ttccaaatga atggttggaa
     1501 attgcagaac catttttcca aaaggaataa aggtgttagg tgattattgt ataattattg
     1561 ctgtttttta atatagtaaa taaatcaggt ataaatataa aaaattattt tagttaatat
     1621 attagtgaaa gcttaatagg aaaattataa atagattaaa ggtagcgcct atataaatag
     1681 ttgctacctt tttctgtttg taaataaatg atagataaga aggtttactg acaaatcact
     1741 gactaaaccc aattttctta cattgaaagc cttaatttta cttgttttaa ataaatttac
     1801 aatttcttgt tataaaatac taaattttgt taacaattat aattagcatt taatttatac
     1861 tggaggttta tatgattagt gtaagaacag atttagcttt agaagctagt gaaatgtgtg
     1921 aaaagtctca agaaggaagt agtattcctg gtgtaaaaat agaaactaaa gaattagaaa
     1981 attgcattgt aactaaagtt gaagtaatag acaggcaagg cgcagagata atgaataaag
     2041 atataggaaa gtatataact ctggaaagta atcttatgaa gtttgatgat gatgagtcaa
     2101 gagaagaaat gataagttat ttaaaagagg agttagtaga tatatttggc caagacaaaa
     2161 ataaaaaaac cctggtaatt ggtcttggaa atagaaatat tacttctgat gcgttaggac
     2221 caaagagtgt atcaaaaaca cttgtaacta gacatctttt taaaaattat aataaagact
     2281 atgatgatga tttcactgag gtatcagctt taagcccagg agttatgggt gttactggaa
     2341 tagaaactag tgaaatagta aaatcattgg ttgagaaggt taagccagat agagtagtag
     2401 caatagatgc attggcttca agaaaaatgg agagagtaaa ctctactata cagatatcaa
     2461 cagcaggaat atcacctggt ggaggtgttg gcaatactag aaaatctcta actaaagaaa
     2521 ctttaggcgt ggatgttatt gcaataggcg ttccaacagt ggttgatgca gcaactctta
     2581 caatagatgt cttagatatg gcaatagaca atttaatagc tcaatcagaa gaaacagaaa
     2641 gtttttatga aatgttgaaa aaacttaaag aagaagaaaa atatcatcta ataaaagatt
     2701 cgttagaccc atatgacaag aacttaatag taacaccaaa ggatatagat gatacaatcg
     2761 aaaatctatc tataataata agtgaaggct taaataggtc tttacatcca ggtagattgg
     2821 tataaaataa ggaggagcaa ttatgtttaa aaaatgcatt aaagtagtaa ccttgacttt
     2881 tatattagta tgcattttac caggaaaatc attagctttg aatcaagatg attttttaaa
     2941 gtttttagta aattcatctt atccagaagc taaagtcgaa ggaaatgata cagaaaataa
     3001 aaagaataat aaaaataaag aaacaagtaa ggaaaataag gaagaaagta aagaagaaaa
     3061 cactaaatct aaagatgcaa gtaaagttga taataaaaaa gagagtgaaa aagaatatat
     3121 aaaactttat gttggtaaag aaaatgttcc agatattgag tcaaaaaatt cagacacaac
     3181 tgaaactaac acaacatcta gttcagatta taaggatgat ttaagagtaa ccaaagaaaa
     3241 tccaagaatt ttgatatatc atactcatgg atgtgagact tattcaaatt cacctgatgg
     3301 aaattatcat tcaagagata agaaaaattc agtaatggaa gtaggaagtg cattaactag
     3361 tgctttagat agcaaaggat ggggtgtagt ccatactact aaataccatg actatccatc
     3421 ttataataac tcttatgcaa gtagcttaaa aacaatacaa agtatattac ctaaatataa
     3481 ctcagtggat atagcgatag acttacatag agatgctagg gatttaacta atccagccac
     3541 taaggaaaaa gaccatttaa aatatacaac tatgataaat ggagagagag tatcaaagtt
     3601 cttctttgtt gtaggaggta aaaatacaaa tagaaaacaa cttagagctt tagcagaaga
     3661 tataactgct tttgctgaga aaaagtatcc tggtctagta tctccaatag tagaaaaaga
     3721 ctatgctaga ttcaatcagt ttgcagttaa gaatcatatg ttagtagaaa tagggaataa
     3781 cgcaacaagt gtagaagaat ctaaggctac tacaaagtat ttagccgaga ttttggatga
     3841 atattttaaa caaaaaaatt agagctttaa gttataatta ggagcatgct aaatgagccg
     3901 attagaaaaa aatatagaaa gtaaaagtaa actaagaaaa tacaagtata ttatgagaat
     3961 cttatttttg attactatga ttataggtac atcagtttgt gtatttatag tagacagtaa
     4021 tgcaaaaagt atgctaggaa tgaaacaata tgattttcca agtattaaat ttgatataac
     4081 aaaatttgat tttgacatac caaattttga tatacatgaa atcataaaaa aatttaataa
     4141 gtagtaataa gtgaataagg tttattgatt aaaataagcc ttattttagt tatatatttt
     4201 taatgtataa aataaaaatt cattaactgt attttatagg atttacttta agtttacgat
     4261 attatataat atttagtgtt agacttgata atccacatat aatgtggata attttgttta
     4321 gtgccaatta gtttatattc aaaaatttta aatttggtat ataaagagtt tttagataat
     4381 gtaaaataat aactataaga ataatcaaga ggagggaatt ttaaggtgga caataaacaa
     4441 agtagaacta gaaattttag tataattgca catatagacc atggaaagtc taccttagct
     4501 gatagattaa tacaacaaac tgggcttgtt agtgaaagag acatgaaaag tcaattactt
     4561 gacaatatgg accttgaaag agaaagagga atcactataa agcttcaaaa cataagatta
     4621 atgtataaag cgaaggatgg aaatgaatat tatttaaatc ttatagatac acctggacat
     4681 gtagacttta actatgaggt atcaagaagt ttagctgcat gtgaaggagc actattagta
     4741 gttgatgcag ctcaaggtgt tgaggcacaa actttagcca atgtttatct tgctatagac
     4801 caagatttag aaatattacc aataataaat aaaatagatt taccaagtgc aagaccagag
     4861 gaagtaaaaa atgaaataga agatttaata gggcttgatt caagtgaagc tcctcttatt
     4921 tcagctaaaa ctggtttaaa catagaagat gttttagaag atattgtaaa aaatgttcct
     4981 ccacctaaag gagataatga agctccatta aaggcattaa tattcgattc ttactatgat
     5041 gcttacaaag gtgttgtagc ttatgttaga gtatttgaag gaactgttaa aaagggtatg
     5101 acaataaaaa tgatgaatac caataaaaag tttgaggtaa ctgaagttgg tgtcatggct
     5161 ccaggtcaaa ctgaacttag tgaattatca gcaggtgatg ttggttatat tgctgctagt
     5221 ataaaagata taagaagttg tcgtgttggt gatacaataa cagattcaaa taatcctaca
     5281 gaagaacctt taccaggata caaaaaagct actccaatgg tatattgtgg tatatatcca
     5341 ggagaaggag aaaagtatga gaatgttaga gatgctcttg aaaagcttca agtaaatgat
     5401 gctgcacttg aatatgaagc tgagacttca gcagcactag gatttggttt tagatgtggt
     5461 ttcttaggtc ttttacatat ggagataatg caagaaagac ttgagagaga atttaacctt
     5521 gatataatta ctacagctcc atctgttata tatagagtta caaaaatgga tggagaggtt
     5581 gtaatgatac aaaatcctgc aaacttacca gaaccatctg agataaagat gatagaagag
     5641 cctatagtaa aaggagatat aatagttcct aaagactatg tgggtgttgt tatggagctt
     5701 tgccaggaaa gacgtggaaa tatgttaaat atggaatata tagatgagag aagagtaatg
     5761 cttcactatg accttcctct caatgaagtt gtttacgatt tctttgatgc tttaaaatcg
     5821 agaactagag gttatggttc tcttgattat gaggttaaag gttatgtagc atctacactt
     5881 gtaaaacttg atatattaat caataaagag caagtggatg cacttagttt tattgtccat
     5941 gagacacgtg ctttccctag aggaaaggca atgtgtgaga aattaaaagg agaaattcca
     6001 agacatcagt ttgctatacc aatacaagct gctgttggta ataaggttat tgcaagagaa
     6061 acaataagtg ctctaagaaa agatgttctt gctaaatgtt atggtggaga tatatctcgt
     6121 aagaagaaac ttcttgaaaa acaaaaagaa ggtaagaagc gtatgagaca aattggttct
     6181 gttgaagttc ctcaaaaagc atttatgtct gtgcttaaat tagatgagta aatccttata
     6241 aatttagata aagatattta aaagctagag tgattttaga attatttcta aattagtcta
     6301 gcttttttat ggtatatttt aagtaaactc atagagaata taagtaagaa tattaaagaa
     6361 gaataaaaag gtgatattta tgattaatat agaaattcct aaaaaagtag actatataat
     6421 taaagaatta gaaaagaatg gttatgaagc ttatatagtt ggaggatgtg ttagagattg
     6481 tctattagaa agagttccaa acgattggga tataacaact agtgctagac cagaagtggt
     6541 agtagaatta tttgaaaaaa caattccaac tggtatacaa catgggactg taacagttat
     6601 gattgaacat gaaccatttg aagtgacgac ttatagaatt gatggaaatt atagtgatgg
     6661 tagacatcca gattcaatag aatttacaaa taacatagtt aaggacttgt caagaagaga
     6721 ttttactatc aattcaattg catacaattc aaagacagga ttagtcgacc catttaatgg
     6781 ctatgaagat atacaaaata agtatatacg ttgtgttgga aatccagtag atagatttga
     6841 agaagatgca cttagaatgc ttagagcagt tagattttca gcacaactca attttaaaat
     6901 agctgaaggt actaagcaaa gtattcataa aaaagcagat ttgataaaaa atgtttctat
     6961 tgaaagaatc cagacagagt ttaataaaat attagtatct gattcatcta aattaaactt
     7021 attaaaatca acaggtttgt tgaaatttat aattcctgaa atatgtgaat tagaagatgt
     7081 tactcaacac aatccatatc acatctatga tgtacaaaaa catactttaa tagctacaga
     7141 agtaattgaa gatgaactgt atttaaaact tacaatgttg tttcatgatt taggtaaaaa
     7201 agtaactaaa acaactgata aaaatggtgt tgaccatttt tatactcata gtagagaatc
     7261 tgtaaagatt gcaaagaaga ttttgaaaag acttaagtat gataattata ctattaataa
     7321 agttttaata ttaatacaat atcatgatta tagaatagaa cctaaaagga agataattaa
     7381 aaagttatta aataaacttg aagatgttga attatttgaa gatttaataa aagttaattg
     7441 ggctgataca cttgcaaaaa atccaaagta tgcaaaacaa aagatattaa atcttattga
     7501 gtgtgaaaaa gaatttaaac atataataaa tcaaaaggag tgctttaatc ttaaagattt
     7561 agctattaat ggaaaagatt tgatatctat aggtataaaa ccaggtaaag atataggata
     7621 tattttaaat aagatgcttg aaatagtcat aaataatcca gagttaaatg aaaaagaaat
     7681 actaaaaga
//
LOCUS       DACXMZ010000189         7680 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.190, whole genome shotgun sequence.
ACCESSION   DACXMZ010000189 DACXMZ010000000
VERSION     DACXMZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.190"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            454..531
                     /locus_tag="JD016_15420"
     CDS             454..531
                     /locus_tag="JD016_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001023.1"
                     /translation="MDIWSPSLEIGRSNDYLEREYTISD"
     gene            986..2344
                     /locus_tag="JD016_15425"
     CDS             986..2344
                     /locus_tag="JD016_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine permease"
                     /protein_id="HAU5001024.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2451..3245
                     /locus_tag="JD016_15430"
     CDS             2451..3245
                     /locus_tag="JD016_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAU5001025.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3238..5544
                     /locus_tag="JD016_15435"
     CDS             3238..5544
                     /locus_tag="JD016_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAU5001026.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPVFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5683..6960
                     /locus_tag="JD016_15440"
     CDS             5683..6960
                     /locus_tag="JD016_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAU5001027.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7143..>7680
                     /gene="yqeC"
                     /locus_tag="JD016_15445"
     CDS             7143..>7680
                     /gene="yqeC"
                     /locus_tag="JD016_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HAU5001028.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKN"
BASE COUNT         3005 a          838 c         1440 g         2397 t
ORIGIN      
        1 ttatagatat atatatctat aaatagatag aaacttgtat attttatgtt gcagatacaa
       61 atatagtaaa ggagagtaca ttggtacaac tgagatacat agcttattaa gtttttctgt
      121 cttatgagaa ttatattata aaatattcat aaatattttg agtttaaatt aaataattat
      181 tttatgctta gattaattga tatttaatta atttaataac aaaaacagtg aaagagtaaa
      241 taatattatt tacagggggt aaagcaaagt atgttaaata aattgtactt ttggccccgt
      301 acatagagaa atctatgtag gacacaactt taaaaccagt aaatcctaaa gccttgcaac
      361 tacaataaaa gatagaaaga aacttttatg aatgtggtga aaaccagaaa caatagtgag
      421 gatggtatat ggtgagagcc taagtactta aaaatggaca tttggtcgcc aagcctagaa
      481 ataggaaggt caaacgacta tctcgagaga gagtacacta taagcgattg atagtggaaa
      541 cgggttgctc ttagcaggta atgctgaaga tgaagaaata gtctacgctc atatgaaagt
      601 atgaggtcac tggatttgaa gtgacatcta taaagtagcg tttataggta aatgtttaaa
      661 acttgaaaca tgtaaatact tatgaaaact tataacttat aatgattaag attgaaagat
      721 ttaaattgaa ttaaatttac tataaaaaat taaaaattca tagctataat actcttatat
      781 ttgtaaagca tggtatataa tgtatacaaa attaaagaat acgtttattt gttaataatt
      841 aaatttattg catctgtttt ttaagactaa aaacttaaat atatgttgga gtatagttga
      901 tatttatgaa catcattaat ttaatatatt agatttttaa gtatttatat aattcaataa
      961 gttttatgta acgggagttt ttattatgaa agcaaaaagt acatcaaaat ttgaattaga
     1021 cggaatacca ccactaaagg aagcaatacc tttagggtta caacacttaa tggcaatggt
     1081 aataggtagt tgtttaccag cgatattgat tgcaaatgca gctggactta atcataagat
     1141 gtcaacatta ttgattcaag ccgcattagt ttttgcagga ttatcaactt tattacaatt
     1201 atatccaata cctttgttta aaggttttaa attaggagga ggaataccag ttataatggg
     1261 agctacagct atgtttatag gagctggggt atctgttgca agtaagtatg gactaccagt
     1321 attatttggc tctcaaataa tagctggtat agtaataatt ataattggtt ttggtcttaa
     1381 aaaaatacga tttatattta ctccagctgt atcaggaaca atagttgcat gtatgggtct
     1441 tggacttttt ccaatagcta taaaaaactt agctgggggt atgggcaatg agacttttgg
     1501 gaatccaatt aattttatag ttggaatatc tgtatctctt atgatattag gattaaataa
     1561 gtatggaaaa ggattattta aagatgcatc aatattagtt agcataattt ttggatacat
     1621 tttatcatta attttgggta tagtaaattt ttcttcaatt caggagttta ctttggttgc
     1681 acttccaaaa ccacttgctt ttgggctaga tataagactt gaagttgttg taatgttttc
     1741 aataatatat ttagttgaga tagctgatat aatgggtgct tgtacacttt ctgcggtagg
     1801 agggcttaat agacaagtaa cagatgaaga actttcttct gctgttttag gaaatggagt
     1861 actctctaca ataggtgcat tattttctgc aactccaatg gggatgtttg gacaaaattc
     1921 tgcaatcgtt tccaatacaa aggttgtaag taaatttgta ctagctattg gaggaattgg
     1981 attatttttg gcaggaatat cgcctttatt agctaattta ataagaacaa ttcctccctg
     2041 tgtggttgga ggagcaacac tcgttatatt ttcaactctt acaacatctg gtttaagact
     2101 tgttagtatg gatggattta atcaagaaaa tagcatgata ttaggtcttt ctatggcttc
     2161 tggtattgga ttcatggtag ctcctcaagt tcttgaaaaa tttccaaaat ttatagagac
     2221 attacttgca gattcaagtg tagtttcagg agcaatggta gccataataa ttcaagcttt
     2281 atatatggta aaatttcctg gaaataaaag tgataaggtt gaggataaaa ataaaagttt
     2341 ataatgtaaa ggtttaatat atgtgaatta aatatactac aattataaat ataaataaaa
     2401 gatttattaa aaacttttgt gttttaataa gttaatgatg gggggataag atggttacta
     2461 taaaggaata ttcagttcca cagagtttag aagaagcata taaaatttta atttctaaaa
     2521 aaaataatgt tattttaggt ggatgtggat ttataaaatt aagtaataaa aatataggaa
     2581 cagcgataga tttaaaagat ataaatctaa attatataaa agaggatgaa aaaaatattt
     2641 tgataggtgc agatacttca cttagaagtt tagaaattga taaaactata aaaaattact
     2701 gtagtggaat actttcaagc gctgtttcta atatagttgg tgttcaattt agacaagggg
     2761 ctagaattgg tgcaagtgta ttttcaaagt atggcttttc tgacttaata ccagcgcttt
     2821 tggttgttgg agcgaaggtg agattataca ataaaggaat actagagtta agtgagtttt
     2881 tggatagtga attaagtagg gatatattgg tcgaagtaat actaccaaaa aaagatgcaa
     2941 tagctgtttt tgactcaata agaaaatgca caggagattt ttctgtatta aattcagcaa
     3001 tgctaaaaga aaatgatact tacaaaatag caattggggc aagacctcaa aaagctagat
     3061 tagcacttga agcgagtaat atattaaata aagaaaaaga tatagataag gcatctattg
     3121 ttgctagtaa ggaacttact tatggaagta atatgagagc tagtaaagag tacagaaagg
     3181 atatggcaag tgcattagtt aaaaggatgt ataatgctat tgaggaggga atgtataatg
     3241 attaataagg ggataagaaa gatagatagt acagcaattg tcacaggaaa acctctatat
     3301 actgaagacc tcattatgca taaagatgtt ttgacattaa aactacttag aagtccacat
     3361 gctcatgcta agataaagaa tatagataca tcaaaagccg aaaaaattcc aggggttgtg
     3421 ggtatttata cgtataaaga tgtaccacag gtcagatatt gtgttccagg tcaaacagaa
     3481 ccagaagcat caccttatga tagagtttta cttgaagata cagttagatt tattggagat
     3541 gaagtagctt tagtggctgc tgaagatgag aaaacagcac ttaaagcctt aaaaatgata
     3601 aaggtagatt atgaggtttt aaagcctgtg tttgatgcaa gagaagcaga aggaaatgaa
     3661 gttgttatac atccagaaaa agacatagta gagccatacc cttttggatt ggatgcaaaa
     3721 aataatatag tatcaaaaga attttttgaa tttggagatg ttgaagctaa gcttaaagaa
     3781 agtgattttg tggttgaatc ttcatacaaa acccaaagtc aatcgcattg catgatggaa
     3841 actcataggt catattcata taaagatata aatgatagaa ttgttgtagt ttcatcatta
     3901 caatcaccac acaatgtaaa gagaatactt agaaaagtat tgggtatacc gtcttcaaaa
     3961 ataagagtaa ttaaacctag aataggtggt ggatttggag gaaagaatat agcagcaaca
     4021 gatatattta ctgcatttgt tactatgaaa actggtagac cagcaaaatt tgtatttaca
     4081 agaagtgaaa cttttgtagg ttctaatagt agacatgaaa tgttttttga tgttaaagtt
     4141 ggtgcagata aagaaggaaa tttaaaagca atagagctaa agtcattaaa caatacaggt
     4201 gcttatggtg ggaatggtgt ctctgtttct tccgaatctg gacacaatac cttaccaata
     4261 tataatgata ttgatgctgt tagatttaat gctagaacag tatatactaa taggttacca
     4321 gcaggagcac ttagagggta tggtgctaca cagggaatat ttgcaataga tagtgctatt
     4381 gatgaattgg cacataagat tggaatggac ccacttgaat ttagaattaa aaatataata
     4441 gataggtata ccaatggaaa agcagctgaa gacgaaatat taagttgtga gctgagacaa
     4501 tgtatagaaa agggcaagaa attaattaat tgggatgata aatatccatg taaagatata
     4561 ggaaacaaca aagtaagagc agtaggtatg gcagtagcaa atcatggttc tggtatacca
     4621 cgtgtagata tggcaacagt cactataaga atggatgaag atggcacata taaactatta
     4681 tctggtcttg ctgacttagg tcaaggggct gatacaattc aagctcaaat tgctgcggaa
     4741 gctttaaata ctactgtaga tagaatttct atttatacag gtgattcaga tttatgtccc
     4801 tatgatacag gagcatttgc ttcttgtaca acaacagtta caggaaatgc aacaataaag
     4861 acagcaaata aattaaaaga tatattactt tcagttgcag agtcaaagct aggatttaaa
     4921 aaagaagatt tagagttggg aaaagacagg gtttttgtta aaaacgataa tagcaagttt
     4981 gttctattag aggaattggg tagagaatct gttggaggaa taggatttgc tagtgatgat
     5041 gtaccagtag ttttggaagc aacagaatct tttggcatgt tgaaaaaaat aaaaccattt
     5101 atagcaggat ttgcagagat agaattagat aaaaatacag gcgaatataa agtcataaat
     5161 tttgcatgtg tacctgattg tggaacagtt ataaatccaa ctttagctag aatacaagta
     5221 gaaggtggtg tgatgatggg tatagggttt gcaatgtatg aagaccctgt atttgataaa
     5281 gatgggagat tattaacaga tagtttccta cagtataaag ttcctacaaa aaaagaagtt
     5341 ggaaatatat tggtagattt tgctgagaat ttcgacccag gaggtcctta tggtgctaaa
     5401 tcattaggag aaatagttat acatacacca gcaccagcta tagcaaatgc aatatttaat
     5461 gctacaggag ttcgtttaag agagcttcct atgacttttg aaaaagttta tatgggtatg
     5521 aaaaataata ataaacaatt ataataaata ataaggtgta taaaagcaag taaatttaaa
     5581 ttattttgta aaataaagta attaaatgga cattatatat ggtgttttgt aaaaataccg
     5641 tatataatgt ctagtagagt atattctaaa ggaggtgagt tcatgtcaaa cagaataaaa
     5701 aatatacact atggaactca gagacaacaa ttaaaaaact taactaaaaa agaatatcaa
     5761 gctctacgag agatgtgctt cttagctaag aacttatata atgttggtac atataatgta
     5821 agacagcatt atttcaatca aggagaacat cttaactatg aagctaatta tcatgtatgc
     5881 aaagaaaatg agaactataa gcttctcaat agtaatatag cgcaacaaat attaaaagaa
     5941 gtagacagtt cgtttaggtc attttttggg ttaattaaat tagccaaaga aggaaaatat
     6001 gactttagag ggataaaatc acctaaatat ttagataaag aagggttttt tagcataata
     6061 ataggtcaaa taagaatcaa agaagatggg attttaaatg tacctatgtc tccagcattt
     6121 aaaaaactat atgggaaagt aagtataaaa gttccacaaa atatactaga gaaaaaaatt
     6181 aaagaaataa gaataatacc taaacataaa gctaggttct ttgaaattca gtattgttat
     6241 gaaataccaa aatcagagga agtttcaaat caaaaaaatg cactagctat agacttgggt
     6301 ttagaaaatt tatgtacatg tgttaccaat ttaggtgata gttttatcat agatggaaaa
     6361 aggctaaaat ctgtgaatca gtgggcaaac aaacagaatg ataaaataga agccttaaaa
     6421 ttaaaaaaga atataactgc aataacaaga aaacaattta aagtttggaa taagagaaat
     6481 aatcaagtta aagactatgt aaataaaacc tgtttttata taattgatta ttgtgtaaaa
     6541 aataatattg gaaatttaat tgtaggatat aatgctaagt tagaaaaaag tgataatatg
     6601 gagagaaaaa tatatagaaa ttttataaga atcccatttg gagagataaa aggaaaatta
     6661 gaatatctgt gtaaaataaa aaatattaat tttattaggc aagaagaaag ctatactagt
     6721 aaatcagatt tttttgctga tgattttcta cctgatatca atgtggacaa tgttaaaaaa
     6781 tatgacttta aaggggaaag aataacacgt ggacagtaca aatccagtgt tggaatcatt
     6841 ataaatgcag atgtaaatgc gagtcttaat atactaaaaa aatcacaagt agttaattta
     6901 tctaatttac agaataatcc tcaaatatta aagcaaccac agagagtaag aatatcttaa
     6961 ttatatttaa aagataaact gatagcgtaa gcttacagct tagcggcgaa ttggatccgc
     7021 cttgttgttt acaacagaat ctatttactt tcgagtttat ggaatttcaa aaatcacttt
     7081 tactaaaaag gtctaactat aataattaag agattagaaa actggttata agaggtgaaa
     7141 gtgtggaact atcaaatata atagaaaaga atgaaataat aactgtagtt ggagcaggag
     7201 gaaaaacttc gtttataaac tattttgcaa atttttatag agataaattg aaagttctcc
     7261 ttacaacaac aactaaaata tatgtaccaa acgattatga caatataatt attacaatag
     7321 atggaactgt tattccatct atttgtcatg gaataacagt atgtggcagt tatataaata
     7381 atgaaaataa attagtaagt attgattcct ctattttgga tgaaattgta gaccaatttg
     7441 atttagtttt aatagaggga gatggctcaa agagaaaaaa acttaaaggt tggaatgcta
     7501 aagaaccagt agtatatcat aaaactacaa aaaccatagg gatattagat ataacatcat
     7561 ttggaatgaa tattaatgaa gagaatatac atagagtaga aatatttaag aaaatagcaa
     7621 atttagatac aagtagtatt aatagtagtt ctacagtaag tattgaaaac ttaaaaaata
//
LOCUS       DACXMZ010000190         7658 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.191, whole genome shotgun sequence.
ACCESSION   DACXMZ010000190 DACXMZ010000000
VERSION     DACXMZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.191"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            47..1759
                     /gene="ptsP"
                     /locus_tag="JD016_15450"
     CDS             47..1759
                     /gene="ptsP"
                     /locus_tag="JD016_15450"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAU5001029.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            2082..3632
                     /locus_tag="JD016_15455"
     CDS             2082..3632
                     /locus_tag="JD016_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAU5001030.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            3658..6360
                     /locus_tag="JD016_15460"
     CDS             3658..6360
                     /locus_tag="JD016_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAU5001031.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNAYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSAQQIVHKQCANDILSCIENCGLDKKYISVEVTEKVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQIAKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(6866..7531)
                     /locus_tag="JD016_15465"
     CDS             complement(6866..7531)
                     /locus_tag="JD016_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001032.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
BASE COUNT         2937 a          762 c         1307 g         2652 t
ORIGIN      
        1 tttataaagt ttatgtgcta gatgatattt tttaggaggt agggttatgg cttacaaagg
       61 aataggtgct tctcctggtg ttgctttagg gaaagcgtta gtagttgaac atagtgaatt
      121 agtaattgaa aaaaaatcta tagataatgt tgaagctgaa attgctaaat tagaagatgc
      181 agttgcagtt tctaaagaag aattagtaaa agttaaggaa aaggcttctg aagaattagg
      241 agcagaggaa gcagaaatat ttgaagcaca tttattagtt ttagaagacc cagagttaat
      301 tggttctgca atagataaaa ttaaaactga aagtgtaaat gcagagtatg ctttaaatga
      361 aataaaagag atgtttgtta gcatgtttga gtctatggac aatgagtaca tgaaagaaag
      421 agcggcagat ataaaagatg ttacaaatag aatacttaga catatattag gaattaaagt
      481 tgttgattta tctgctttaa gcgaagaagt tgtattaata gcacatgatt taactccatc
      541 agacacagca actatgaata aaaaaatggt tttaggattt ttaactgata taggtggaag
      601 aacttctcat actgctatca tgtctagaac tttagaaata gctgcaatcg ttggattaaa
      661 tgatataact tcaaaagtta aggatggaga ttttgtggtt ttcaatggag acacaggtga
      721 agtaatcgtg aatcctgatg aagagactat aaataaatat actgaattaa aagctaagta
      781 tgaagatgag agaaaagcat tacagctatt aaaaggaaaa ccatctgtaa ctttagatgg
      841 aaaacatgtg gaattagctg gtaacatagg aacacctaac gatatagaag gtctaataaa
      901 gaatgatgct gaaggtgttg gtctttatag aacagaattt ttatacatgg atagagattc
      961 tttcccaaca gaagaaatac aatatgaagc atacaaagct gtattagaag gaatggatgg
     1021 aaagcctata gttataagaa cattggatat aggtggagat aaagaattaa gttatttaag
     1081 tatggaacct gaaatgaatc cattcttagg ttacagagca ataagacttt gcctagatag
     1141 aaaagatata tttaagactc aattgagagc tttatataga gctagtgttc atggaagatt
     1201 aagaatcatg ttcccaatga tttcatcttt agaagagttg ttacaagcta aagaagttgt
     1261 aaaagaagtt ttagcagaac ttgattctga aggtgtggct tatgctaaag atgtagaaat
     1321 tggtatgatg atagaagttc catctgctgc tgtgatatct gatgtattgg ctaaacacgt
     1381 agatttcttc tctataggaa ctaatgattt aattcaatac acttgtgcag ttgatagaat
     1441 gaatcaaaag ataagttatc tttacaatca atttaatcca gctgttttaa gacttataaa
     1501 gactgttata gacaatgcac ataaagaagg aaaatgggca ggtatgtgtg gagaatctgc
     1561 tggagaccaa aaaatgatac caatactttt aggtatggga ttagacgagt tctctatgtc
     1621 tccaatatca atacttcctg ctagaaagtt aataacttca gttaaagagt cagatatgaa
     1681 aaaattagct gatgatgtac ttaatatggg tactgctgaa gaaataaaaa gctatataga
     1741 aaagactttt aatatataat taatgttata tagttactaa aattcatttt tctaaataac
     1801 aaaattatat tttacgaata aaaaaactat agtatatcat atatactata gtttttttgt
     1861 tatataaatt acgtatcaaa atttgtaaaa tgaatagata taattactaa atatgtgata
     1921 gtattggcta taataggata taaaaaataa tattgtaata aaaagtttat atttagtaat
     1981 gaaaatttaa cgttttactt gaaattatgt gttttgtaat gtattattaa ggttctaata
     2041 ggaacatttt catcaatttt tctatagtgg gggtaggaat tatgaggtac aaaaaaagaa
     2101 atatagataa gtcaagtgaa ctttcatttc aatctctagc aaaatatttt ataactttct
     2161 atatagtaat tatattaatt attctttcta tgttttgtat aaatacaata tgggttatga
     2221 aaaaagataa tcaagattta ttatctacaa cgtcaaatcg tattgaagat agggtaggag
     2281 aaacaattaa gctattgaac tctttgagta aagataatta cataatagat aatagtgtga
     2341 atggcataga taaaagtaaa gaactttcaa tgtatagtga tgaatttggg tatatgatga
     2401 tatgttttgt tgataagaat ataattgtat gggatgaaga aggtggtaat attagtctag
     2461 ctggtcgtga ttatatgcag aaactttttg caacaaaaaa atatcaagtt acagatagtt
     2521 ttgcagcagg ggctgatgga aaaactttaa actacactat tgcatcacct ataataaaga
     2581 atggaaatgt agatggttgt gtatttgcag caatttactt tgatgagata aaggatatac
     2641 tcaaacaagg aaaaggtcaa cagcaacaga atttcatatt atttggaagt gaaaatcaaa
     2701 taatgaattt aacaagtgat gaaactgcat atggagagaa gtatatgaag ttatcgcaga
     2761 aaacaaaata tcttggaacc aatgcaaaaa aagtagaaaa ggatatgttt aataataaat
     2821 caggtgatta ttgggctttt gaaaatggaa aattatactt tatgtcctat aaaccaatta
     2881 agaacacaaa ttggagttta ttttgtaaag ttaatttctt agatattttt gcattaaatt
     2941 taattcctat gggagctatt ctatctgtta taactttagt atttattatt ttgatttgta
     3001 tggagaaaag cttcatgaaa aaacgaatgg aagttattga tatgctttta ggttctgttc
     3061 aagaattaga aaaacgtata tatcaaacag aaagacctag tgaagtagac tttaaagaaa
     3121 ttattcagct cacaagtaaa ggtctgactg atggattaac tggtgttgta acacgtactg
     3181 tattttttga ccaaatttat aatcgtgttg aaatggagga aaatgataaa ttatctgtaa
     3241 tttcatttgt tgatttagat gatttgaaat gtattaacga cttatatgga catgaaattg
     3301 gggatatggt tttgaaaaat gttggatatg tacttcgtga atatgagata aaatataatg
     3361 gtttaattgg gcgctatggt ggagatgaat ttgttatgtt gttatctggt tttacatcga
     3421 gagaagaggt ggaggattta ttggatgaga ttgctattcg cttacatact gaagtactaa
     3481 cagatgatgg tatcattcaa actcattgta gtgttggagc tgtagtatgt agtgtaaaag
     3541 acaataaaga tatagataat ttattaaaac aggctgatga agctttatac tatgttaaac
     3601 gtaatggaaa aggtaattat catttagttt aatgtatgtg gaaggagttt atatcatatg
     3661 aaaaatgtat ctaaaaaatt ttttgtatgt actattattg tattaataac atttttatgt
     3721 tttagtaaat ttgctattta tgtttttgac aaacaagaaa agaaagaaac aaaagtagta
     3781 gaaaacatgt taaatgccta caagaataac attattttaa atctgactga aaaatttaat
     3841 tatagcaaag acttgacatc agttttagtt tcaaacaatt attctataga taaattaaaa
     3901 cctattgcag atgaaattct taaaaacaat tcttgcatat atatttcagt tgtagaaaaa
     3961 tataatgtag ctgcattttt ttctaattct gtcaaaaaag atattatcaa tactccaata
     4021 caggaactga attattctta ttcattagca aattctatca aaggattagt cgtagaaggt
     4081 ccttctaaag accagttttc aaatggagat gtgtttttaa ttactaatcc tatttataaa
     4141 aatggagagt actggggaga aattacagta gctttagatg ccaaaaaagt tgtagattct
     4201 tttaacttaa ataaattaaa agaatctgga tttgagtatg aactttggcg tgtacaccta
     4261 gataatggtt caaaatctac aatagaaatt tctactaaag attttaattt ttccaaatca
     4321 catgaaatta agtttaaact tccgactgtt tggactctta gcattgtgta caaaaatgga
     4381 tggaataata agaatataga ttttattgta tttgttatat tgttaattat ttccatatta
     4441 ataggtgtga tgacatttat ttacattgaa aagaagtcga ttagtaagga attaaatcaa
     4501 cagagtctta aaaatttaga aactggtatt ttgaatagag aaggtttttt aattaagtta
     4561 caagagcatc tgaatagaaa aaataatgaa atagtgttgc tgtatatatg tattactaat
     4621 tatagtaaat ttgctccagt tatgagcttt gaaaaaagac aagattatct tacacatata
     4681 gtaaaaaatc tagataatta cataaaaagt tctcatttta ttgctcgtat aagtgaggaa
     4741 ggttttgttc tagctttaga agaaaattgt gataaaaatg aattgcttaa cattgaaaaa
     4801 gggatttcct tagagctttt acgaaaaatt aaagtagaaa ataaacaagt atttttaacg
     4861 attggttatg gctcatctag gtgtgttgat gatagaaaag atgcagaatc tctgttactt
     4921 tcagctattg aagattatga gagaagattt ccagcaatgt ctcctattca tgattttact
     4981 aaaaagtgtg aagatatgct tgatgaaaag aaacagattg tatttggtga atatccagat
     5041 ttagaaataa acaagttgtc taaagtaata tacaaatatt ataaaaaagt acagcgtatt
     5101 atgtataaag attataatat tgatattgga aataggcgag ggtatatgcg tgatgtcgaa
     5161 gtattaattg attataattc tgagagaatg ttatacttat ttttaataga catatgcaag
     5221 tttggaaaat ttaatgaact gttttctatt gaaataggag atgttatttt aaaagaagtt
     5281 agtaacaaat taaaaacaat tttttctaat tatctatatc gtattaatgg agatgtattt
     5341 ttaggcattt caatagataa agaatcttac ttagatactt tacataaggt taatgaagta
     5401 tttaatagtt ctttaagtgt agaagatgta ctttttgatg taaaatgtaa tattggtatt
     5461 tgtcaatatc catatcatgg tgaaaatcca tatgaccttc tggaaaaggt acaaattgca
     5521 ttggagtatg caaaaaagga tgtcaaaagt aatgttaaag tttatgataa ggaattgaca
     5581 gaagagattt accatgaaaa taatattatt aaggatttaa aaaaaggtct ggaagaacaa
     5641 acactagaag tttggtatca gccaattttt aatgttagta aaaactgtta tataaaagct
     5701 gaggcactaa ttagattgag aaataaagag ggcggttatt attctgccta tgaagttatt
     5761 acttatgcag aaaagcatgg acttattaag gatgtaggtc tatatgtact aaaaaaggct
     5821 tgtgatgtaa ctacaaaaat aaaagataaa aatatagact ttgaacaaat gcaattaaat
     5881 ttatctgctc agcagattgt acataagcag tgtgcaaatg acatattatc atgtattgag
     5941 aattgtggtc tagataaaaa gtatatttct gttgaagtta cagaaaaagt acttatggaa
     6001 acttttgaaa ttgtaaatgt tactcttgat aaactaaaaa aagaaggcat tagcatagct
     6061 ttagatgagt ttggttctgg atttagtagt ataaattatc tggcaaattt acctattgaa
     6121 aatttaaaaa tagataagga attagtagaa caaattacct attctaaaaa acaatttgaa
     6181 ttattaaaaa ctatagttca aattgctaag attaatggca tggatattgt agcagaaggt
     6241 gttgaaacaa aagaaactct ggatttagtt gtaacttcag gtgtgaattt gattcaaggt
     6301 cattattttt cagaaccttt aagtgaagat aaatttattg aacttatgat tgaagaataa
     6361 aggttaataa aataaagatt taaggtattg aaaaataatt aaaagtattt aataatagaa
     6421 ttagggaaga ataagtttat aagtaaaaac aattgattag tagttaataa aataaatgtg
     6481 atatccaaat aaatcctaaa tctaatttga gttttaatat agatagtcta aagtttgatt
     6541 attaaagatt taattgtaag ttgttacagt tgatgatatt gattagagat taaagtttag
     6601 agtagaaagt taccttaaat ttaaaaatgc aagataaccc aatctaaaaa ttataatcac
     6661 tcgataggtt taaggattta tttgtttatt ataaatagga gggtgtctca aaatgaactt
     6721 tttagtttat gaggcactct cttttatata gaattaaata tattttatta tttcaacaat
     6781 gaaaaagaag cttatctcta ttttgagaca gcctctttgt atactattat taatttaatc
     6841 aataacataa tttaaaatta taatactagt taagatttcc tgctttatta gtaaataatt
     6901 ctttaatagc attgtttctg gaaactaaat cgttatcaga gttgtttgtt tttttcataa
     6961 tcactgaata acgtttttta tttataaaaa atattaaaat cttatcatca tcaaaattgt
     7021 attctttata tctgtcgata gactcataac ctagatttag aatgtttttg tcttttatac
     7081 tatctacatc taaaatatat gatgggtcag cctttagtaa tatattaatt aaacattttt
     7141 cttcaccagc acagaataac tccatcttac ttgatataga tttagattgc tctgaagtta
     7201 tttttgcagg aataaggttg tcattatcat ctcttagaag ataaccatgt gtgtcttgtt
     7261 gatatactaa attaaccttt ttcttaggaa cactaatcat tttatctaaa tgtacataat
     7321 gatgattttc aacaaaacct tcatttaata tttcaacaaa gttattaatt ttacctaggc
     7381 aaaaagattc tatagattct ttacagaatt tatttgtaaa tggtattatg tttacgtttt
     7441 cctcgtctgt attccatgct atagcataat ccaacgaata tacttcttcc tctatatctg
     7501 caaaattata ttgtactatg tttcctttca aaattactcg acctccatct tataatatat
     7561 ttaatatact ttataaaact tatattatat ttaaataata tcataaattg aataaatatg
     7621 atatagtaaa taagaaaaaa tttgaaaaaa atcactat
//
LOCUS       DACXMZ010000191         7656 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.192, whole genome shotgun sequence.
ACCESSION   DACXMZ010000191 DACXMZ010000000
VERSION     DACXMZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.192"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            230..1531
                     /locus_tag="JD016_15470"
     CDS             230..1531
                     /locus_tag="JD016_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAU5001033.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1607..1882
                     /locus_tag="JD016_15475"
     CDS             1607..1882
                     /locus_tag="JD016_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAU5001034.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3249..3479
                     /locus_tag="JD016_15480"
     CDS             3249..3479
                     /locus_tag="JD016_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAU5001035.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3472..4053
                     /locus_tag="JD016_15485"
     CDS             3472..4053
                     /locus_tag="JD016_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAU5001036.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(4633..5310)
                     /locus_tag="JD016_15490"
     CDS             complement(4633..5310)
                     /locus_tag="JD016_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAU5001037.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5499..6863)
                     /locus_tag="JD016_15495"
     CDS             complement(5499..6863)
                     /locus_tag="JD016_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAU5001038.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTIFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            6991..7512
                     /locus_tag="JD016_15500"
     CDS             6991..7512
                     /locus_tag="JD016_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAU5001039.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         2988 a          870 c         1320 g         2478 t
ORIGIN      
        1 atatattaat aattggtagt aaagtatatc aaataagata ttgctattat gtagtaaatt
       61 atataaaaca atgtatcaga ataaatagta tattaataat ttgtagtata tcatgacgaa
      121 taatacagtc ataacaaata gtgagtcata acaaataatg tatttataat tgataatgta
      181 tttaaaaatc aaaatataaa aacttataag gaaagagaga ttaatttata tgggtaaatc
      241 aaaagatgtt gaaatagaga agtctatttt aggcacaatt cttttagaca ataaattaag
      301 ctataagcta gatgaattaa atgaaaacat gtttatgaat gatatatgtc ttgagatatt
      361 taaaattatg aaagaactta aaaaagaaaa catagtgatt gatgtggcca ctgtgaaatc
      421 aaagatagat agaaaatctc tagcgataaa aacaagtgat gtgactaatc taattacttg
      481 gggtcagaat tttggtcttg atggtcatat taaaatttta aaagaaaatt tagctagaag
      541 aagcataaat caaaactgcc aaaacctttt acacagccta aaccttggag aaaatatcga
      601 tacttgtatt tacaaatttg aaagtaatat aaaggagatt ttggacaagg atacttatga
      661 aaatgatgat gtaaatagta tagcagggaa ggtgcttgat tttttagaaa acaaaaaaga
      721 tattggattt aaatttggta taaaattact tgataccact ataggaggtc tttttaaagg
      781 agagctaacc acaatagcag ctaaatcggg ggttgggaaa actgctttgg cacttcaaat
      841 aatgttaaat tcatttaagc aaggtaaaaa aactttattt ataagtagag aaatgaccag
      901 tgagcaagta tttatgagaa atatatgcag agtaactggt gtaagtacaa ggaatatgaa
      961 atctaaagaa atagatgaaa atgattggaa acttattgta aatgctatag gtgatttaag
     1021 cgaaaataac ttaatttata ttaatgataa gatagataca attagtgcaa ttagaaagag
     1081 gataaggcag gtaaagccag atttattaat agtggattat gtgcaactcc ttacatctca
     1141 gaaaagcatg gataagagag aaagggaggt agctactttt tcgagagaac ttaaaaatat
     1201 gactttagat tttaatatac ctgtaatcca attaagtcag ttaaatgatg aaatgaaaga
     1261 ttcaagacct tatggagata gacctatgag ggatagcaaa gccatctacc acgatagcaa
     1321 caatgtggtt tatatacatc aattaaaggg gagtgattat gaagaagcag tgagagatat
     1381 tggagaaagt gaggaggcag ttagagcatc agaatacagg ggaattaaga tggttgattt
     1441 aatagttgcg aagtgtagag atggacagac taggcataag catttttgtt actttgggga
     1501 taagttacat tttcaagaac taaattatta ggtcaataaa gtagtaataa caagcttatt
     1561 aaaagtcggg aaatagcaaa tagattataa aatatgaggt gaaaatatgt tggaattaag
     1621 aaaaattata gaattacttg aagaacacaa gatagaagta agtattccaa aggagttaca
     1681 taagtatagt ttattggaat accttgaatt gtgtcaacaa agtgggggaa catattttta
     1741 tattaaaaca ctggaagggg ctttaaaaga ggctagaaat gagcttatga ttaaattgtt
     1801 taatgggaaa aattatatgg aattagcgag gatgtttgaa atgagtgtta ttaatgtcag
     1861 gcatattgtt agaaaaaaat agatattttt ggagaataca agtgtgatat acttctgaaa
     1921 tagaagttat tatagttgaa ataaaattgt ggttaataaa ttaaagttgt gtataagttt
     1981 aagattatag aggagtataa gtgtgattaa cagcatgatt ttgtgaataa aattgaggat
     2041 atatatgagt taaatgtgtg attaataagg taaggttgtg tataagatta tgagtattta
     2101 aaaggagggg ctataattaa tagtgtagag ttgtgtgttt gcttaagatt atataataat
     2161 ttaatagatt aaactatact aataaggtaa aatcgtgtgt ttagttaaag cttattataa
     2221 aatcttgata tgtgaataaa gttaagatta taatagattt aacctgtggc taatagaata
     2281 aaattgtgaa taaagttaag attataatag gtttaatctg tgactaatag agtaagagtg
     2341 taaatagaga taaacttcaa tatgagctta acttgtggtt aatagagtaa ggtgtaaata
     2401 gagataaact tcaatatgag cttaacttgt ggttaataga gtaaggtgta actaaagata
     2461 aacttcaata tgagcttaac ctatgattaa tagagtaaga atgtaaatag agataaactt
     2521 caatgtgggt ttaacttgtg gttaatagag tagggtgtaa ctaaagataa acttcaatat
     2581 gagtttaact tgtgattaat agagtaaggt gcaactaaag ataaacttca atatgagctt
     2641 aacctatgat taatagagta agaatgtaaa tagagataaa cttcaatatg agcttaacct
     2701 atgattaata gagtaagaat ataaatagag ataaacttca atatgggctt aacttgtggt
     2761 taatagagta aggtgtaact aaagataaac ttcaatatga gcttaaccta tgattaatag
     2821 agtaagaatg taaatagaga taaacttcaa tatgggctta acttgtggtt aatagagtaa
     2881 gagtgtaact aaagataaac ttcaatatga gtttaacttg tggctaatag aataagattg
     2941 tgaataaagt tatagtttta gatgggtatt aacctgttac taatagaata tgattaggtg
     3001 taaaattata gagctgtata agaaaatatt atagatatat taaatattag ataataaata
     3061 tttatataga tacacaaaag aaaaggcgat atgaagctaa acttaatgac ttcatatcgt
     3121 tttttatttg aaactccttt gtatttagag tgtgattttt aatattgagg ttttatgtga
     3181 ttaagtcaca gataaaaagt aagtatcttg atataattct tatagattaa taatataggg
     3241 aggttattat gtctaataaa aaaaagaagt atgcaaatat tttagcaaat gagtgcgttg
     3301 cttgtggtag ctgtataaaa gcatgtccaa gaagtgctat ctcagttcca tgtggcatta
     3361 gtgcaaaaat taatagagat ttatgtgttg gatgtgggat ttgtgaaaag atatgtccag
     3421 catctgtaat tgagattata acaatattaa aagaggaaga aggtaagtgt catgaataat
     3481 aaaaaacatt ggtatgacta tttatggatt ttttcttcaa tatatttgat attgggattt
     3541 gtaaatatta ttttcgcttg gattgggcta atttgtttct ttgttccact agctatttct
     3601 attgttaagg gaaataaagc ttactgtaat aagtattgtg gaagagggca actgtttaat
     3661 ttgcttggaa ataaattgaa gctgtcaaga aatagagata ttcctaaatt tattagaagt
     3721 aagtggttta gatatgggtt tttgacattt tttatggtaa tgtttgctaa tatgcttttt
     3781 tcaacttatt tagtgtttga aggaagtaga aattttaaag aagtagtgac tttgttgtgg
     3841 actataaaaa tgccttggca atgggcgtat catggaactt tagtttcacc ttgggtggta
     3901 cagtttgcat ttggttttta tagtgttatg cttacatcaa ctgttcttgg tttgattact
     3961 atgatattgt ttaaacctcg ttcatggtgt gtttattgcc ctatgggtac tatgactcag
     4021 atgatatgta aggcaaagag taagtctgta tagaaaggcc ttatgtgata tttacagttt
     4081 tgttcaagta gagatgagta gtaggtttga atatatatga caaatatatg agtatagggg
     4141 catagattta gtttgtgtaa ggcaaactga tttatgcttc ttttttatgt cttagattat
     4201 tggctaagta gaaacaatga atttagatat taagaattat tcagttattt gaagaaaata
     4261 gaataataat taacattata tgaatggata ttcattacat ataagccagt taatagcaag
     4321 atttatttaa tatgaaatag gtcaagtatt taaactaaat aaggtcatga caattaatat
     4381 aagtatagat ataaaaagat tagaagttat aacttataaa aggtattcta tataaatagt
     4441 atggagaaaa tagtcaaatt aacttattag aaacaaaaaa tatacataaa aaaataaatg
     4501 taaatatcaa ataagatact atataaaaat agatataagt atctttttta ttattgaaat
     4561 tatgaaaata tatttgactt tacacaaaaa agagcgcctc atgaactaaa aagttcattt
     4621 tgagacacac ttttacagtg tcaaagaacg aagtttatcc ttatccaata cttttatacc
     4681 accacgagat aaagacacaa taccttctct agcaaaatat ttcaacattc tcgatacaac
     4741 ttctctggca ctacccatat acttagcaat ttgttcatga gtcatattta tagtgctact
     4801 gccattttta gctatctcat caattaaaaa agatgccaaa cgcttatcaa aactcataaa
     4861 aagaatttgt tgcatagccc acataacatc agaaaaacgt tcggtagcta atttatatga
     4921 aaataactct gcatgaatat tttctgtaga taattttgca aaaacagagg aactaatctg
     4981 tataatatcg caatccgttt cagcatctac atagacatca aaagttattg tttttagaat
     5041 acaggaagct gataaaatac atacatctcc atcatcaagt cggaacagtg taacctctcg
     5101 accttcatct gaaagtatgt atgtacgcac tgttccagac tttataatca aaataccaat
     5161 acagtcacta tcaccacaat gtactggatt cccttttttg taatgcgagg tatttgctga
     5221 agaaattaat aattcttttt gtaagtcagt aagtttatcc caaaaaggca agctatgtgt
     5281 aagtatatca atttcatttt gtgaaatcat acaatatctc ctttcttaaa ttgttataat
     5341 cccatgatac aagtgaatag ataaattgac aagtagtttg ttaagaggat tatttagtca
     5401 gatagtaata gtatgattta tgtagaaaaa atatgataat atatattctt gctattaatg
     5461 agaaagtaat atgttttgat gatagtaggt ttgtataact aatgcatacc gtttattttt
     5521 aaaagattaa gttccttatg taaccttata gacataaaac cagcaattat agcagccatc
     5581 aaatcagcta ttggttgtgc atataagata ccatttaacc catagaatac aggaataatc
     5641 aaaattatag ggataaaaca gacaccttgt ctacacatac ctaaaaatcc accttccttt
     5701 gcttttccta gtgcgaggaa taatgtagaa tacactgtat agaatccaaa tagcataaat
     5761 gaaaacccat ttgccctaag agctttacta ccattttcta ttaatagaat gtcaccactt
     5821 gtaaattgtg aaattataga ttttgggaag agtatcaata taagaccaag cactacacaa
     5881 aatattgtag tccataatat ggaagttttt attgctatat gtaatctatc gtagttcttt
     5941 gaaccaaaat tataaccagc aattggttgg aaacctttta taaaaccaaa tacaacaaga
     6001 ctacctaaag atgttattcg tgttacagca cccattccag ctatcacaga atcaccatac
     6061 tttgtagcct ccatattagt taatgtaatg gcaagactag tcaataattg aaatattaag
     6121 gtaggtatgc caatttttag gatttctgaa aaaatttctt ttgaaaaaca gcattcgtta
     6181 attctaaaac taaatatact tttcttgctt aaaatatatc ctacataaac aagtgttgaa
     6241 gcaatctgtg agatagctgt tgcaatagaa gcacccctta cccccagacc aaaagtataa
     6301 atgaatatcg ggtctaaaat catattaagc aaagcaccta ataacatagc acacatagtt
     6361 gttttagcag ctccttcact tgtgacaata ttagtcatag tgacattgaa tatattaaaa
     6421 atagatgaca cgacataaat gctagcataa gatattgcat aaggcataat actttttgta
     6481 gctcctaaca attttaaaat tggattgaga aaaatcataa tcacaataat aactaatgta
     6541 cccactataa tactactgta aatagatgta cttaccactt ttcctgcttt ttcattatca
     6601 ccatgtccta aaagtctggc aatatatgaa gcagctccat ttccaaacaa taatcctaat
     6661 cccacaacaa gctgacctaa tggaaatgca acggatatag ctcccatttg acttgttcca
     6721 agtccaccca caaagtatgc atcaaccaaa ttgtataatg cagttatcat catacctatc
     6781 atggtaggta atcccataga taacaatgct tttgagattg gggcagaccc taataattca
     6841 attcttttgt tactttcttt catgcttaac actcctcttc tattgacatt aagtctaaat
     6901 tataatacta taaattatag taagtatgga aaatagtact ataatttata gtatttgtca
     6961 atagtaagaa aactaaagga gatgattttt atggctacaa tagatttaat tgtattggga
     7021 atagtaaagc aacaacccaa gagtgcttat gatattcaaa aggcagtgga atacaggaat
     7081 atatcacgtt gggttaaaat aagtacacca tcaatttata aaaaagtaat tcaattagaa
     7141 caaaagggat atattgaaag taaaactgtg aagaatggaa aaatgccaga aaaagcagtt
     7201 tattcattaa ctgaattagg tgagacacat tttttagatt taatgtttga tacatctagc
     7261 aaaccaataa atattctttt agattttaat gctgttatag taaatttaaa tagccttaca
     7321 ccagaaaaac agaaattatg tctcaataat attgaagaga atgttcaaga gttaaagaaa
     7381 actattgagg aaaatataag tataaagaca gaagtaccag agacagcaaa agcagtgttg
     7441 gagcaacagt tggtgctagc acaagcattg gaaacctgga taaattcttt aaaagataat
     7501 atgggaaatt agaattagag aagctaactt aataatcatt aaacaaaaac cagcatattt
     7561 aataatgaga tattagaaaa aataaaacaa atgatataaa ctgaaaagta tatttaaaat
     7621 aaagctcact taattgaggg agaatgttaa actttt
//
LOCUS       DACXMZ010000192         7566 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.193, whole genome shotgun sequence.
ACCESSION   DACXMZ010000192 DACXMZ010000000
VERSION     DACXMZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.193"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            92..168
                     /locus_tag="JD016_15505"
     tRNA            92..168
                     /locus_tag="JD016_15505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:126..128,aa:Arg,seq:tct)
     gene            178..253
                     /locus_tag="JD016_15510"
     tRNA            178..253
                     /locus_tag="JD016_15510"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Gln,seq:ttg)
     gene            262..337
                     /locus_tag="JD016_15515"
     tRNA            262..337
                     /locus_tag="JD016_15515"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:295..297,aa:Lys,seq:ttt)
     gene            340..428
                     /locus_tag="JD016_15520"
     tRNA            340..428
                     /locus_tag="JD016_15520"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:374..376,aa:Ser,seq:tga)
     gene            432..507
                     /locus_tag="JD016_15525"
     tRNA            432..507
                     /locus_tag="JD016_15525"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:465..467,aa:Phe,seq:gaa)
     gene            514..587
                     /locus_tag="JD016_15530"
     tRNA            514..587
                     /locus_tag="JD016_15530"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:548..550,aa:Met,seq:cat)
     gene            602..678
                     /locus_tag="JD016_15535"
     tRNA            602..678
                     /locus_tag="JD016_15535"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:636..638,aa:Ile,seq:cat)
     gene            696..772
                     /locus_tag="JD016_15540"
     tRNA            696..772
                     /locus_tag="JD016_15540"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:His,seq:gtg)
     gene            781..856
                     /locus_tag="JD016_15545"
     tRNA            781..856
                     /locus_tag="JD016_15545"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:814..816,aa:Lys,seq:ttt)
     gene            864..937
                     /locus_tag="JD016_15550"
     tRNA            864..937
                     /locus_tag="JD016_15550"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:896..898,aa:Cys,seq:gca)
     gene            945..1021
                     /locus_tag="JD016_15555"
     tRNA            945..1021
                     /locus_tag="JD016_15555"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:979..981,aa:Arg,seq:acg)
     gene            1034..1109
                     /locus_tag="JD016_15560"
     tRNA            1034..1109
                     /locus_tag="JD016_15560"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1067..1069,aa:Val,seq:tac)
     gene            1185..1598
                     /locus_tag="JD016_15565"
     CDS             1185..1598
                     /locus_tag="JD016_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001040.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            1907..5338
                     /locus_tag="JD016_15570"
     CDS             1907..5338
                     /locus_tag="JD016_15570"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAU5001041.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5580..7508
                     /locus_tag="JD016_15575"
     CDS             5580..7508
                     /locus_tag="JD016_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAU5001042.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3008 a          932 c         1504 g         2122 t
ORIGIN      
        1 agttcagtgg tagagcgcaa ccttgccaag gttgaagtcg cgagttcgaa tctcgtttcc
       61 cgctccaaat tttatgttta tgttgtaatg tgggtgcata gctcagctgg atagagcaac
      121 gcccttctaa ggcgtgtgtc cggggttcga atccctgtgc gctcaccaca tatatattgg
      181 ggattagcca agtcggtaag gcacatgact ttgactcatg tatgcgtagg ttcgagtcct
      241 gcatccccag ccaaacagta tggtctatta gctcagtcgg tagagcacct gacttttaat
      301 cagggtgtcc cgcgttcgag tcgcggatag atcaccaatg gagaggtgtc cgagtggttt
      361 aaggagctgg tcttgaaaac cagtgacttc gaaaggggcc gtgggttcga atcccaccct
      421 ctccgccaaa tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg
      481 gtggttcgat tccgcctctg ggcaccaaaa tgtggcggta tagcttagtt ggctagagcg
      541 ttcggttcat acccgaaagg tcataggttc gactcctatt accgctactg aaatttaatg
      601 tgggccatta gctcagttgg ttagagcgcc cggctcataa ccggtaggtc tggggttcga
      661 gtccctgatg gcccaccaca ttaaattaac ttatggtggg tatagctcag ttggttagag
      721 cgccagattg tggctctgga ggtcgtgagt tcgactctca ttatccaccc caacttatat
      781 ggtctattag ctcagtcggt agagcacctg acttttaatc agggtgtccc gcgttcgagt
      841 cgcggataga tcaccaaaaa ggcggcgaca tagccaagtg gtaaggcagt ggactgcaac
      901 tccttgatcc ccagttcgaa tctgggtgtc gcctccaata aaatgcgctc atagctcaac
      961 tggatagagt gtctgactac gaatcagaag gttaggggtt cgagtccctt tgggcgcacc
     1021 atatgaatag cgagggatta tagctcagct gggagagcac ctgccttaca agcagggggt
     1081 cacaggttcg agccctgtta atcccaccag tgcaaacctc tagtaaaact actagaggtt
     1141 ttatttttga ttgaaaaagt ttttatattt aggaggtaaa aaatttgtat atagtaatag
     1201 gaaatgaaat agttgatagt gaagaactta aaataattat agataaaaat agtgaattta
     1261 aagttgaaaa agatttaagt aaaagcacaa aaagagaaga tgtaatagca tatcagttaa
     1321 gtatagattt aaattaccta gattctctta taaatgagca atgtaattta gccagtctat
     1381 ctgatgaaga gaaatttgat gaatatatga ctttgtctga tgaacttgca ctggatttag
     1441 aagagttaat gccaaaatat acaataataa atgctagagc atataaatta gacgaggtag
     1501 atggaattgt aaaaataata ctagctgtag cgtatgcaga tttaggacac ttaaaacttt
     1561 ctgatgtagt aaagaggcta tctagacaag tagattagga tatgaatatg aactatatta
     1621 aaaaaatgta taaaatctta cgtaaagatg aaaataatta tgtaaacata gaaaaaaaca
     1681 gtataaaatc aagtcaattg tagaaatttt cacataaatt catctgaaat tctataaaaa
     1741 atgccttaat tttaatgagg taaatcgagt ttacacagtc taaaaattac ttatatttat
     1801 aaatttatct tattaattaa ggaactttct taaacatctt caaaaataaa gtattttagt
     1861 ctataatctt taatattaca caaacaataa aggagaagaa tagagtatgc ttaaaaagtt
     1921 taataaaata ctagtggcta atagaggcga aatagctata agaatattta gagcctgttc
     1981 agaattgggg ataaaaagtg ttggtatata cagtaaggaa gataagtatg gattgtttag
     2041 aacaaaagcg gatgaatcat atttgatagg agaaggtaaa gggccaatag atgcatattt
     2101 agatatggat ggaataatag acctagcaaa gagaaaaaaa gttgatgcta tacatccagg
     2161 atatggtttt ctagcggaga atgcagaatt tgctcgtaaa tgtgaagaga atggaattac
     2221 ttttatagga ccatcatcta aggtaatgaa tatgatgggg gataagatta actctaaaaa
     2281 aatcgcaaaa gaagttaatg ttcaaactat accaggagta gaaaaagcaa taagaagtac
     2341 tgaagaagca aaggaagttg caaacaaaat aggatatcct gttatgataa aagcatctaa
     2401 tggcggtggc ggtagaggta tgagaattgt acatcgcgaa gaagacttag agcttgaata
     2461 tgaaactgct tgtagtgaat caagaaaagc ttttggagaa gatattatat ttatagaaaa
     2521 atatatagct gatccaaaac atatagaagt tcaaatatta ggtgataatt atggaaatat
     2581 agttcactta tatgaaagag attgttcagt tcaaagaaga catcaaaaaa taatagaata
     2641 tgcaccagct ttttcgttag atgataaagt aagaaaagag atatgtgaag atgcagttaa
     2701 gttatcaaaa catgtgggtt attctaatgc gggaacatta gaatttttag tagatgcaaa
     2761 tggtgggcat tattttatag agatgaacac aagagtacag gttgaacata ctgtaacaga
     2821 gatggttaca ggaatagata tagttcaaag tcaaatacta atagcccaag gttacagctt
     2881 agatagtgaa gaaattaata taaaatcaca agatgatgtg gaaataagag gttactctat
     2941 ccaatgtaga ataactacag aagaccctaa gaataaattc atgcctgata caggtaaaat
     3001 tcaagtttat agaactggtt caggatttgg tataagacta gatggaggaa atggatttac
     3061 aggggcaaac ataagtcctc attatgatag tttattggtt aagacaatat cttgggatag
     3121 aacattccaa ggagcaataa ataaaactat aagatctata aaagaactta gagttagagg
     3181 tgttaaaact aatgtaggat tcctagttaa tgttttaaat aatcctatat tctcaaatgg
     3241 taaatgtagt actaagttta tagatgaaaa tccagattta tttgaaataa cagaaagtaa
     3301 agacagagga acaaaattac ttcaatttat aggagatgta attgtaaatg ataatgcttg
     3361 taaagaaaaa ccattatttg atgcattaca tgacccaaga atggataagg atggttcaaa
     3421 atcagaagga agtaaaatac ttttcgataa gttaggtaaa tctgcatata tagaaaagat
     3481 aaaaaatgat aagaaattac tattaacaga tacaacaatg agagatgcac atcaatcatt
     3541 attagcaaca agaataagaa catatgactt attaaaagct gctaagccaa cagaaaaata
     3601 tcaaaaggat ttattctcac ttgaaatgtg gggaggagct acttatgatg ttgcatatag
     3661 atttttaaaa gaatctcctt ggcgtagatt acaaaagcta agagaagaaa taccaagcat
     3721 aatgttccaa atgttgctta gagcatcaaa tggagttgga tataaaaact atccagataa
     3781 tgtaatagaa gaatttacaa aagaatctgc tagacaaggt atagatgtat ttagaatatt
     3841 tgattcatta aactgggttg aaaatatgaa accatctatt aatacagctt tagaaactgg
     3901 aaaaattgtt gaagcaacta tgtgttatac aggtgatata ctagataaga ctaaaactaa
     3961 atacaatcta gaatattata ttaaaatggc acaagaatta gaaagtttgg gagcagatat
     4021 aatagcaata aaagatatgt caggtcttct taaaccatat tcagcttaca cactagttaa
     4081 agagttaaag aaaaatgtaa aagcaccaat acatttacac actcatgata caagtggaaa
     4141 tggtgtagca acttgtttaa tggcgtcaga agctggagta gacataattg atgctgcatt
     4201 agaatctatg gcaggactta caagtcagcc atcacttaat gctatagtag aggcacttaa
     4261 gaatacagag agagatacag gaatagattt atttggatat gatgagttag gaaaatatta
     4321 taaggattta agaaaagttt ataataaatt tgaaagtgat ttaactaatt cttgtgcaga
     4381 aatatataat tttgaaatac caggaggcca atatacaaac ttaaaacctc aagctgatag
     4441 tttaggatta gttaatagat ttgatgaagt taaagaaaaa tataaagaag ctaatgaagt
     4501 tgtaggagat ataataaaag taactccatc ttcaaaagtt gtaggagact tagctatctt
     4561 tatgactaaa aataaattag ataaagataa tataatagaa gaaggtaaaa atttatcatt
     4621 cccagattct gttgtggatt actgtaaagg tatgattggt caaccagaag gtggaatacc
     4681 aaaagattta caagaggtag ttctaaaagg tgaagaagct ataacagtaa gaccaggttc
     4741 attattacca gcagaagatt ttgatgaaat agctaaatat ctaaatgaaa agtatgatat
     4801 aaatgctaat ataagaaatg ttataagtta tgctttatat ccaaaagtat atgaagatta
     4861 tatcaaacat ctacaacatt ataatgatat atctaaactt gaaagtgatg tattcttcta
     4921 tggattaaat aaaaatgaag aatgtgaagt agaaatagaa gaaggtaagg tattaacaat
     4981 aagattagtt gaaattggtg aagttaagga aaatggattt agaactatag gatttgagtt
     5041 aaatggaatg gttagagaag tagaaataaa agataagaat ttctctggaa aaattaataa
     5101 tgttgaaaaa gctgatatga atgaccctct tcaaattgga gcaagcatac caggaaaagt
     5161 tataaaaata atggttaaag aagaagatga agtaaaagca aatcaaccat taatcgttat
     5221 agaggctatg aagatggaaa ctataatagt tgctaagact gatggagtaa ttaagtcaat
     5281 caaagtaaaa gaagatgata tggttgaaga taaacaatta ttaatgataa tgaagtaatt
     5341 taattaaaat aaattaaaaa gcactctgaa atgtttaata aaaaatgttt tagagtgttt
     5401 ttatttattt taattaaata tttgttaaaa aaaagaggac aattacctcc agatgtagaa
     5461 ataaagctag taaggaaaat gttatctaaa aattctaaat atagtaaata ttttccataa
     5521 tatttcttag gaaataacag caaagataaa atacataaat ttaaaaggtg ggtgcttata
     5581 tgaaggttta tgatagtaaa atgttgagaa atgtagcggt attaggacat agcggatgtg
     5641 gaaaaacaaa tctaatcgaa actatagctt acacagcaaa cactaataag atacctaaat
     5701 taacagacaa ggtaaatatg acttatagca tgggattaat acctatagag tataatgatt
     5761 ataagtttaa cttattggat accccaggat attttgactt tagcggagac gttgtgtcat
     5821 ctcttagagc aagtgatgca gcaataatag taatagatgc aacagcgcct attcaagttg
     5881 gtacagagaa atcattagaa ctaactgaga gtattcctaa gataatgttt ataaataaaa
     5941 tagataatga aaaggcaaga tacaaagatg caatagcaat gttaagagaa aaatacaata
     6001 ataagatagt tcctatgata agtcctatat acaaagataa aaactttgta aagttacata
     6061 atgtttttga aaacatagat gatttagaag gtgaatttaa ggaacaagct atgagtgtta
     6121 aagaagcatt aatggaactt atagcagaaa ctgatgacca aatattagat aaatatttta
     6181 atggagaaga actaactaca gaagagatac aaaaaggtat tattataggt atacaaagag
     6241 gtgatattat acctgtaata tgtggttcaa ctataaataa tataggcaca aaggaaatat
     6301 tagatacaat atcaagttat ttagaaccta tctttacaga ggaaagcaaa ccatttaggg
     6361 gattagtatt taaaactatg gttgacccat ttgttggaaa gatgtcatat ataaaaatta
     6421 ctgagggtgt actttctaag gataaagatg tttttaatat aaataaaaat gtaaaagaaa
     6481 aaatagctaa tatatataca ttgagaaata gtgaattagt agaaatagaa aaagctaaag
     6541 ctggtgatat agtagttata actaaagtta attctctaaa aacaggagac actatatctg
     6601 cagataaaga tgcagaagct ttagaaaaga tagattttcc taaaccacaa atatattatg
     6661 cagtaactcc taagaataaa ggtgatgaag aaaaagtagc atctgtatta aataaactag
     6721 tagaagagga ccctacatta cattggtata gaaatacaga gacaaaacaa gcacttttag
     6781 gaggtcaagg agaacttcat ataaaaacaa ttaaaaataa aatgaaagat aagtttggtg
     6841 ttgatgttga attgaatgac cttaaagttc cttatagaga aactataaaa ggaacagctg
     6901 atgtacaagg aaaacataaa aagcaatcag gtggtcatgg tcaatatgga gacgtaaaaa
     6961 taagatttga aagatgtgaa tctgattttg aatttacaga agaaatattt ggtggttctg
     7021 taccaaaaca atatatacca gcagttgaaa aaggtttaaa agactctatg caaaaaggta
     7081 tattagcagg atatcctgtt acaaacataa aagctacgct ttatgatggt tcataccatg
     7141 atgtagactc ttctgaaatg gcctttaaaa tggcagcaag tgcagccttt aaaaaaggta
     7201 tggaagaagc acacccaata ctactagaac ctataatgaa acttaagata actgttccag
     7261 aagaatatat gggagatgtc atgggtgata taaataagag aagaggaaaa atatttggaa
     7321 tggaaccaga tgacaaaggg aaacaaataa tatttgcaga agctccacaa gcagagacat
     7381 ttaaatatgc aatagattta agagcaatga cacaaggtag aggatacttt gaaatggaat
     7441 tagagagata tggagaggtt ccatcacagt ttgcagaaaa aataattggg ctagctactg
     7501 caaagtaatg aacaaaataa atagaaaaat aattaatata tattattaaa aatattttta
     7561 gggttt
//
LOCUS       DACXMZ010000193         7469 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.194, whole genome shotgun sequence.
ACCESSION   DACXMZ010000193 DACXMZ010000000
VERSION     DACXMZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.194"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            99..1076
                     /locus_tag="JD016_15580"
     CDS             99..1076
                     /locus_tag="JD016_15580"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAU5001043.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1069..2037
                     /locus_tag="JD016_15585"
     CDS             1069..2037
                     /locus_tag="JD016_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HAU5001044.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLVVSALIAVNALGDVYENGKVIAGALNDKRTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2221..2811
                     /locus_tag="JD016_15590"
     CDS             2221..2811
                     /locus_tag="JD016_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAU5001045.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2830..3600
                     /locus_tag="JD016_15595"
     CDS             2830..3600
                     /locus_tag="JD016_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HAU5001046.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3737..4705
                     /gene="dusB"
                     /locus_tag="JD016_15600"
     CDS             3737..4705
                     /gene="dusB"
                     /locus_tag="JD016_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAU5001047.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4814..5293
                     /gene="greA"
                     /locus_tag="JD016_15605"
     CDS             4814..5293
                     /gene="greA"
                     /locus_tag="JD016_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAU5001048.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5308..6837
                     /gene="lysS"
                     /locus_tag="JD016_15610"
     CDS             5308..6837
                     /gene="lysS"
                     /locus_tag="JD016_15610"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAU5001049.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7059..7340
                     /locus_tag="JD016_15615"
     CDS             7059..7340
                     /locus_tag="JD016_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001050.1"
                     /translation="MKIFRIIYEKVKRIIYENTIYKKLLGECLMFQVINFLCLFCVGF
                     LVLDVMIYFCKDAMDIFEGTTNVNKVKSINVWADRVRANSYSYENKIAK"
BASE COUNT         2974 a          755 c         1515 g         2225 t
ORIGIN      
        1 gaaatttaaa attaatagta ttaaaagtat attaataatg tataattatt tattagaaga
       61 aaataagtca tgaaattatg tcatattaga ggtgaaatat gcgagataaa ataatagaga
      121 ttatattaaa taataaatct gaatttattt caggagaaga attatcaaaa caattagggg
      181 tgtcacgtgc agctatatgg aaacatatga aagccttaaa agaagaggga tataatatag
      241 aatctgtaaa taaaaaaggg tatcgattag cggaaaatcc aactgattta ttaagcccac
      301 agaatattta ctataaatta gatacagagt ttattggaaa aaatattgaa catcttgaca
      361 ctatagattc aacaaatgaa catgctaaaa ggatagctat aaatagtgta gatgggactg
      421 tgattttaag tgaacatcaa gatatgggga aaggacgatt gggaagaaga tgggattcaa
      481 aacctcatga aggaatctgg atgagtatta tattaaaacc agatatggaa ccattcaaag
      541 ccccatttat aactttaata gcaggagcaa gtgtaacgaa agctttaaac aatctaggag
      601 caaatacatt aataaaatgg ccaaatgatg ttattttaaa tggaaaaaaa atatgtggta
      661 tattaacaga gttatctgct gaaattgaaa gaacaaatta tgttgttctt ggaattggaa
      721 taaatgttaa aataatggat tttgaacaag aaatagctga caaagccaca tcattgtata
      781 aagaagggta tatgttatcc agagtagata ttgttaggaa tgtattgact gaatttgaaa
      841 aattatattt agattatact gtaaataatt ccaaagaaaa aaccttaaaa atatgtagag
      901 attattctgc aattatagat aaaaatatat atgtattaaa aggagataat agagaactag
      961 tgaaatgttt agacataaat gaagagggaa atttattagt tcaaagaagt gatggaagta
     1021 taaaagaaat tatttcaggt gaagtttcta taagaggaga aaaaggatat gtataataat
     1081 attttggata tagaaggctt aaaggttggg caggttgagg atagaaatgg tcttactggg
     1141 tgcacagtag taatatgtga agaaggagct acttgcggtg ttgatgtaag gggctctgct
     1201 ccagggacta gagaaaccga tttattagac cctgtaaata tggttcaaaa agttcatgca
     1261 gtagttttgg ctggaggttc tgcatatggt cttgaatcca cttgtggtgt aagtagatac
     1321 ttagaagaaa aaggaattgg ttttgatgta ggtgtaggta aagtgcctat agttgtagga
     1381 gcagttttat ttgatttagg agtagggagc tttaaatgta gaccagattt aaagatgggt
     1441 tatagagctt gtgaagttgc aaatactgat attttaaatc aaggaaatta tggagcaggt
     1501 tgtggagcca ctgttggaaa gataagaggt caggaatatg ctatgaaagg cggaataggt
     1561 agctattcta taaaattaga taatggtcta gttgtatctg cattaattgc tgtaaatgct
     1621 cttggagatg tttatgaaaa tggtaaggtt atagctggtg ctttaaatga taaaaggaca
     1681 gaaacaattg atacatataa attaatgaaa caaggtgaaa gtaaaggtgg atttagtgta
     1741 gataatacta caattgggat aattgcaaca aatgctaagt taaccaaagc tggatgtaaa
     1801 aagatatctc aaatgggaca tgatggatat gctaagtcta tttttcctat ccatacacct
     1861 catgatggag atacaatatt cacaatatca acaggagaaa tagaagcaga tataacatta
     1921 gtaggttcat tggctgtaga ggttgtggaa aaaagtataa taaatgcagt aaaaaaagcg
     1981 gatagtgtag aaaatataat ggcatataaa gatatagtaa aaaaacgaga aatatgatga
     2041 aattagcata tttttcttgt caaattgttg acaatggcaa ctcatcttga taaaattatt
     2101 cttgttagag cgggcatcct acaatggtag cttgactcat agttataaga aaaaaacgaa
     2161 aaaaattaaa aaattaagta gtccggcatc ttaatgagct ggaacggagg tgtatttttt
     2221 atgatgaaca caagtgcatc agtaagaaaa gtaaatgtaa gaaagatgac tataatagga
     2281 gtactatcag ctatttctat tatgctatct atgactccat tgggatttat tccagttgga
     2341 cctacaaaag caactataat gcacatacca gttattattg gagctattat ggaaggacca
     2401 atagttgggg cagcaatagg atttatattt gggatatcaa gtctcttaaa tgctataaca
     2461 aacccaacag taacatcttt tgtttttata aatccattag tttcaatatt accaagagtt
     2521 atgataggtg tattagctta ttatgtatat cagttaataa ttaaggctac aaataaggta
     2581 tacatatcag gtttaattac aggggcaata ggttctctgt taaatacagc tggagtttta
     2641 ggaatgatat atgtcttata tgctgacaag tatcttcatg caatgggtaa aagtggttca
     2701 gcaggaaaag taataatggc tcttgccgca gcaaatgggg tgccagaagc aatagtaggg
     2761 gctttagtag tagctgctgt tgccactgta cttaagaaaa gcaaaaaata gaaagaaggt
     2821 taatgacata tgcttctagt atttgatgtt ggaaatacta atatggtttt aggtatatat
     2881 aaaggtgaca aattagttaa ttactggaga attaaaacag atagggaaaa aacgtctgat
     2941 gaatatggaa tcctgataag taacctattt gattatgata atgtgaatat aagtgatatt
     3001 gatgatgtta taatatcatc tgtagttccg aatgttatgc attctcttga aaacttttgt
     3061 ataaagtact gtaaaaaaca accattaata gtaggtccag gcataaaaac aggtctaaat
     3121 ataaaatatg ataatccaaa acaagttggg gcagatagaa tagttaatgc tgtagcaggg
     3181 atagaaaagt atggagcacc aagtatactt gttgattttg gaacagcaac tacattttgt
     3241 gctatctctg aaaaaggtga atatttgggt ggaacaatag caccaggaat aaaaatatct
     3301 agtgaggcgt tatttcaaag tgcgtctaaa ttacctagag tagaattagc taagccaggt
     3361 atgactattt gtaagagtac tgtatcagcc atgcaatctg gaataattta tggatatgtt
     3421 ggtttagttg acaaaataat aagtatgatg aagaaagaat tgaattgtga tgatgttaag
     3481 gttatagcta caggtggatt agctaaactg attgcttcag agacgaaaag tatagattat
     3541 gtagatggtt ttttaacact agaaggattg agaataatat atgaaaaaaa ccaagaataa
     3601 ttaatataca gggaattggg ttatatatcc aattcccttt ttatgataga tttctagcta
     3661 tatgtggtat attataatga ggaaataaga atttaaagtt aaatcacaaa ataaaggcaa
     3721 aaataggaga aaaacaatga aaataggaaa tttagaattg aaaaataaag tttttctatc
     3781 acctatggca ggtgtaacag accttccatt tagattaatt tgtaaagaac agggatgtgg
     3841 gcttttatat acagaaatga taaatggtaa ggcactttgc tatgatgatg aaaatactaa
     3901 aaaaatgctt aagatagaag aggaagaaca tcctgtagca gttcagatat tcggttcaga
     3961 acccgagttt atgggaagag cagcagagat aatgaatgat tattcaaatg agattctcga
     4021 tataaatatg gggtgtccag ctccaaaagt agttaaaaat ggagatggtt cagcacttat
     4081 gaaaaatcct aagcttgcag aagaggtatt gagggcagta gtgaagaact caaaaaagcc
     4141 agtgacttta aagattcgaa aaggctggga tgataatagt gtaaatgctg ttgaaatagc
     4201 aaagatagca gaggattgtg gaataagtgc tttagcaata catggaagaa ctagagaaca
     4261 gttttataca ggcaaagcag actgggatat aattgcagag attaagaaaa atttgagtat
     4321 acctgttata ggaaatggtg atgtatttac aatagaagat tcaataaata tgttagacaa
     4381 aactggttgt gatgctataa tgataggtag gggtgctcaa ggtaaccctt ggatatttaa
     4441 aagaataaac cattatatga atacaggcga gattttgcca gagccaactc ttaacgaaaa
     4501 aataagtact gccattaagc atttaaaact tgcagtagaa gaacatggag agtatgtagc
     4561 tgtaagggaa atgagaaaac acatcgcttg gtatttaaaa ggacttagaa actctgcaaa
     4621 attaagggat gaaattaaca aaatagaaga ctatcaagaa gttgtgtcga aattggagta
     4681 ctatatggaa gactccttga cataaaaata atagtgactt ataatatagc gcataaatta
     4741 aaaaaagatt ataaaatttt taaaatataa ttaggaaaat tcctaagaaa tttgtgaagg
     4801 agttgtattt ataatggaag aaaataaaga gtttttattg acacaagagg gatatgataa
     4861 attagaagaa gaattagaaa acttaaaggt tgtaaagaga aaagaagttg cagagagaat
     4921 aaaagttgct atatcttttg gtgatttatc tgaaaatgct gagtatgatg aagctaaaaa
     4981 ggaacaagca caagttgaag aaagaatttt aaaattagaa aatatggtta gaaaagctgt
     5041 tatcatagat gaaagcaaaa tagaccttaa tgttgttaca ataggatcaa tagttaaagt
     5101 taaggattta gaatttgatg aagaggttga atatactata gttggttcga cagaagcaga
     5161 cccatatgat ggtaaaatat caaacgaatc tcctgtaggg aaagcacttt taggtagagc
     5221 tgcgaaagaa gttgtggaag tgcaagttcc tgatggagtt gctaagtttg aaatattaga
     5281 aataagaaga taatttggag gaaaaaaatg aaaaataatc aacaaagcaa tgaagaagcg
     5341 caaattcaag aagaccttag tgaagtactt caagtaagaa gagataaact aaaaaaatta
     5401 caagagtcag gtagagatcc ttttaaagaa agtagatatg atagaactca ttattctatg
     5461 gatataaaag ataactttga ttctcttgag ggtaaaacta caaaaatagc aggacgtata
     5521 atgagcaaga gaatacaagg gaaagctggc tttatagata ttcaagacca agagggaaga
     5581 atccaatctt atgtaagact agatgctata ggagaggaag aatatagtgt attttcaaca
     5641 tatgatattg gagatatagt tggaattgaa ggagaaatct ttaaaactaa aaaaggtgaa
     5701 atatctgtaa aggctaaatc tgtagtatta ctatgtaaat ctttacaagt tttaccagaa
     5761 aaatatcatg gattaaaaga ccaagaacta agatatagac aaagatatgt tgacttaata
     5821 gtaaatccag aagtaaaaaa tgctttttta attagaacaa aagcattaaa agcattaaga
     5881 gcatacttag atgatagagg atttttagaa gttgagactc caatattaaa tactatagca
     5941 ggtggggcaa atgctagacc atttataact aatcataata ctttacatat accaatgtat
     6001 ttaagaatag cgaatgaatt gtatttaaaa agacttattg ttggtggatt tgataaagtt
     6061 tatgaaatgg gaagaatgtt tagaaatgaa ggtatggatt taaaacataa tcctgaatat
     6121 actgcaatag aattatatca agcatatgca gactatactg atatgatgga aataacagaa
     6181 aatgttattg cacatatggc tgaagttgct acaggaagta tgatagttaa ctatcaagga
     6241 acagaaataa actttacacc accttggaaa agaatgagta tggaagattg tgttaaagaa
     6301 tattctggtg tagatttttc tactataaat acagatgaag aggcactaga agttgctaga
     6361 gaaaaaggta tagagataaa acctggaatg agaagaggag aagtaataaa tgctttcttt
     6421 gaagaatttg gagaagataa attaatccaa ccaacattta taactcacca tccagttgaa
     6481 gtatctccat tatctaagag aaatgttgaa gacccaagaa gaactgatag atttgaggca
     6541 tttgctaata agtgggagtt agctaatgca ttttctgaac ttaatgaccc tattgaccaa
     6601 aaaggaagat ttatagacca attgagaaaa agagaattag gtgatgatga agcttttgaa
     6661 atggatgaag atttcttaaa agcattagag gtaggtctac ctcctacagg aggactagga
     6721 ataggtatag atagagtaat tatgttatta actaactctc catctataag agatgtatta
     6781 ttattcccga ctatgaaacc tattgataac aatccaaata aagaagaaga aaactaagaa
     6841 atattagcat tctactaagt tttaaataaa gtttagctat taaattatca ataaatagct
     6901 aaattttatt taaaatgtca gtgtggaatg ctttttatat gcttcaaaga ttcttttaga
     6961 tatttaattt ataaattacc aacaaacgtt agaaattgat attagttttt gttgattgag
     7021 actttatatt tagaaaatat gattgaaagt aatttattat gaaaatattt agaataattt
     7081 atgagaaggt aaaaagaata atttatgaga atactatata taaaaaatta ttgggggaat
     7141 gtctgatgtt tcaagtaatt aattttttat gtttattttg tgttggattt ttggttcttg
     7201 atgttatgat atatttttgt aaggatgcaa tggatatttt tgagggtaca actaatgtta
     7261 ataaagttaa gagtataaat gtttgggcag atagagtgag agctaattca tattcttatg
     7321 aaaataaaat tgcaaaatag gtaaaattat atatgcatgt attgtttgta atatcaaggg
     7381 ttatatctat tagttttaat tatatttatt aaaaaaacca aaagttataa ccttttttaa
     7441 aataaaaaac aaaaaaagta ttgacgaaa
//
LOCUS       DACXMZ010000194         7402 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.195, whole genome shotgun sequence.
ACCESSION   DACXMZ010000194 DACXMZ010000000
VERSION     DACXMZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.195"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..935)
                     /gene="bclA2"
                     /locus_tag="JD016_15620"
     CDS             complement(<1..935)
                     /gene="bclA2"
                     /locus_tag="JD016_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HAU5001051.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGPTGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGSDGAVGP"
     gene            complement(1313..1852)
                     /gene="hpt"
                     /locus_tag="JD016_15625"
     CDS             complement(1313..1852)
                     /gene="hpt"
                     /locus_tag="JD016_15625"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAU5001052.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRHINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2180..3463)
                     /locus_tag="JD016_15630"
     CDS             complement(2180..3463)
                     /locus_tag="JD016_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAU5001053.1"
                     /translation="MEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIVENRMLVSP
                     SIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIAEDYMDNTP
                     LSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNEPKVSASND
                     AAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQKTGIGVGYG
                     IKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTAILPIVAYN
                     KKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGISWLMNGTEK
                     QIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVPPLNGIVGS
                     SVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            3688..3843
                     /locus_tag="JD016_15635"
     CDS             3688..3843
                     /locus_tag="JD016_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase"
                     /protein_id="HAU5001054.1"
                     /translation="MEKHMTISQKDKKKSQNEILYHFETAQMYYFLTYKNHFLIHKKR
                     FCKTLIV"
     gene            complement(3912..5687)
                     /locus_tag="JD016_15640"
     CDS             complement(3912..5687)
                     /locus_tag="JD016_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAU5001055.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GVSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(5899..6468)
                     /locus_tag="JD016_15645"
     CDS             complement(5899..6468)
                     /locus_tag="JD016_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAU5001056.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(6652..7287)
                     /locus_tag="JD016_15650"
     CDS             complement(6652..7287)
                     /locus_tag="JD016_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAU5001057.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREISDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2154 a         1452 c          813 g         2983 t
ORIGIN      
        1 ggacctactg ctccatctga tcccgttggg cctgttgctc ctactgctcc ggttggacct
       61 attgccccag ctactcctgt tgctcctgtc gctcctgttg gacctaccaa accatttgct
      121 ccggttgctc cggttgggcc tgttgggcct accaaaccat ttgctccggt tgctccagtt
      181 gggcctgttg ggcctgttgg acctaccaaa ccatctggac ctgttgctcc tactgctcct
      241 gttggaccta cctctccatc tgctccggtt ggacctgttg ctcctattgg gcctgttgcc
      301 ccagttgttc cggttgctcc agtcgctcct atacttccgg ttggacctgt tgctcctgta
      361 tttcctgttg gtcctgtagc acctgttact ccatttgctc cggttgctcc tgtatttcct
      421 gttggacctg ttattccatt tgctccggtt gctccggttg ctcctgttag tccggttgga
      481 cctgttgctc cagttgctcc tgtattccct gttggacctg ttattccatt tgctccagtc
      541 gctcctgtat ttcctgttgg tcctgtagag cctgttattc catttgctcc agttgctcct
      601 ttgtttcctg ttggacctgt tattccattt gctccggttg ctcctgtatt ccctgtcgga
      661 cctgttattc cattcgctcc agtcgctcct gtatttcctg ttggtcctgt agagcctgtt
      721 gttccatttg gtccagtcgc tcccatattt cctgttggac ctgttattcc attcgctccg
      781 gttgctcccg tatttcctgt tggacctgtt attccatttg ctccggtcgc tccagttgca
      841 cctctaggcc ccgtcggtcc tgttggacca gtagcacctg ttggccctgt tggacctaca
      901 tcttgatata aacttggacc tgaaatatca ctcatataat taatcctcct tttttaaagt
      961 tagagtatta ctctataaat attgagtatt actctatata attatatgaa tcgacaattt
     1021 cttatgtgat aatgtatcta tttgcttata tcaacatata tgctttgatt cttgaatttc
     1081 attacttttt tgtaaaatct tttacaatta ttagtactct tttacaatta ttaatacaat
     1141 taaaaaactc atttcccatc aattatttaa aattttttta ttatgttata aaaaatagat
     1201 aattaaattg cataaaatta atttaatact actttaatta ctttgtaaat aaaaaaacct
     1261 caacacgatt aataaaaatc atattaaggc atccacaaaa ttatataatt gcttatctat
     1321 cttcatcagt aacattgaat atataaggaa catttctata ataatcacca aaatcaaacc
     1381 catatccaac cacaaactta tcttctattt caaatccaca ataatctggc actaaatcga
     1441 cttttcttct gcttggttta tccaaaagaa cacagcattt taaactagca ggatttttag
     1501 cttttagatg ttttagcaca aaatccatag taagtgcaga atcagtaata tcgtctacaa
     1561 ccaaaacatc atatcctgct atatcagtag tcacatctga aactacttta acccttccag
     1621 tactttcttc attgtttcca taactagatg tagtcataaa attaactcta agtggtaaat
     1681 ttatatgtct aactaagtct gcacaaaata taaagctacc ttttaataga gaaactacca
     1741 taagattttt atctttataa tctttttcta taattgctcc taattccctt aatttatctt
     1801 taattttttc ttctgaatac aatacttccc atttttttgt ttctatatcc ataaaaagac
     1861 ctccaattgt aataatcctt cttaatttta taagagattg ctcacttttt caatactctt
     1921 attcaatatt agtcaatatt attgagtttt tatgttcatt caacaattat taacaatgta
     1981 tcttatattt taagtagttc atatatccac actaaattac atactatata ttttagacaa
     2041 aaaaagaaaa aacttgcact aacaagtttt ttctattata cataaattta tacataatca
     2101 tattttatta gagggtacta attaaattta tttatgctat ttttgttata cctcataata
     2161 caaaacatat tataaagtac tagtcaactt tattcatatc attcattaca tttaagatta
     2221 tctcatctgt tatagacata cctttgtcac ttactctacc taagttttgt atactttgct
     2281 caacacttga accaactata ccatttagtg gtggtacaaa acaatcttgc ttagctatta
     2341 tagcactttg tatagctgca gatgctgcag aagataattt taatgcacaa ccagctttcg
     2401 caccgtcaca tatcattcca cttaagtcag ctatcatatt ttctatagct ccttctattt
     2461 gcttttcagt accattcata agccaagaga taccagcagt tgctccagta gaagcagcaa
     2521 caccacatcc acatacagca gataatcttc ctgtatagtt cttaatataa ccagttacta
     2581 gatgagatat agctaaagct tttgctagtc tttcatcaga ttgtgggaat tttttattat
     2641 aagcaacgat tggaagaatt gctgttaatc catgatttcc acttccattt gaactcataa
     2701 caggcatttt tacccctgcc attcttgcat ctgaagctcc agcagttaac atcattgcat
     2761 aatttataac atcattacct aaaagacctt cttctataga ctttttaatt ccatatccta
     2821 caccaatacc agttttttgt tttaatccat attcagccat ctcttcattc atttttactc
     2881 catctagtaa aaattctata tctttaaaat caattccttc tacatttttt attaattctt
     2941 gtatagttac tgtatccatt aaactttcag ctttttctgc cgcatcattt gaagctgata
     3001 cttttggttc attgtctaat aatacttctc catctttttc taagaacgta aaattatcat
     3061 gtttagttct tattctcact ttagcaatat gattttttcc ttttaacaca acttctataa
     3121 atactttctc tcttgtatca gcaggtgtta ttgatagtgg agtattatcc atataatcct
     3181 cagctatttt aacttcctct tttgttagag tttctaacac acttaatcca ttttcagaat
     3241 gtccacctac tatacctaga gctgctgcaa tttttaatcc taatctttca gtacctggta
     3301 tacctacgca cataccattt ttataaatac taggactaac taacatacga ttttcaacta
     3361 tttcttctcc taataattcc tttgcctttg cacatgctag agctaaggct actggttcag
     3421 tacaccctac tgctggttta acttctgcct ttaacatttc cactaattct tttcttatat
     3481 ctctcattat atataaacct cccaattaag tttagattta gtactcaaat aattaattaa
     3541 ataatttctt atctatattt atagcaaaat acatgccata ttttttatat ctttttatat
     3601 ttttagtctt atattctata accattggta tgactgcttc tatttatgta ttttttttat
     3661 ttacatagag tatttatatt ctttaaaatg gagaaacata tgacaatttc gcaaaaagac
     3721 aaaaaaaagt ctcaaaatga gattctatat cattttgaga cggctcaaat gtattatttt
     3781 ttaacttata aaaaccattt tttaattcat aaaaaaagat tctgcaaaac acttatagta
     3841 taagtttaaa gctttcttta aagttatgaa tttagaaaaa tttaaatttt atatagcttt
     3901 ttattatatt actatgaaat tatattgtat ttttcaattt ttctatataa tgtagctctt
     3961 gatattccaa gtgctttagc tgttaattct ttatcttttt tataatcttt gtacttattt
     4021 aaagctttta ttatctcaat cttctctagt tcatcaagtg gaattatcct atcgctttta
     4081 acttctaact cttctacaga ggtattctta attttttgag gaattacatt cattgtgatt
     4141 attgaagaag aagacatatt cacactatat tcaataatat tctgcaactc tcttatattc
     4201 cctggccact tatattccat catagcatca acaaccctgc tatctatgtc aataacattt
     4261 ttatctaatt tttttgaata ctctttaacc atatgcttga ttataagtgg gatatcttct
     4321 tttctctctc ttaaacttgg taattttata ggtataacat ttaatctata gtataaatct
     4381 tctctaaaag tgccattttg aaccattttt tctaaatctt tatttgtagc agcaataatt
     4441 cttacatcta ttgaaacatt tgatttactt ccaattttat taagttcctt ttcttgtaat
     4501 actctcaata actttgactg taaatgcaaa ctcatatctc ctatttcatc taaaaatata
     4561 cttcccttat gagctacttc aaacattcca agctttccac cttttttagc tccagtaaaa
     4621 gctccctctt catatccaaa taattcactc tctaataaat tgtctggtat agcagcacag
     4681 tttacagcta taaaaggatt atctactcta ttgctatggt tgtgtatggc tcttgcaaat
     4741 agctccttac ccgttccact ttccccagta ataagtactg ttgatgttga tttagaagct
     4801 attagggctt cctttttaga atttcttata gctacacttt ctccaattat gttttccaat
     4861 tttattctat aatctttatt catcttattg tagtttttta tagcatctct tttatctata
     4921 aaatcaataa catatccctt taactcattt tttaatacta ttttattgat gttgtatatt
     4981 cccttcgaat catatccgta tcttttataa aaaaaactac aggatttgtc tttcttaaag
     5041 ttatttatag aaggtttctt tataaaatct aattcgacaa atatgttttt gccagttata
     5101 ctatccttta tattgaaaag ctctttaaat tttgaattat atttatcaat ttttccatcc
     5161 acatcaatag acacaacagc tttgtccata ttgtttaaca gtgtctctaa ttttttcttt
     5221 tctaattcaa gctcataagt tttaatttgt gcttttaatt tattagatat taggtctgcc
     5281 attttgccta aaaagtttat aagaccactt ttattatttt ctataatctg tgcttgttcc
     5341 tttgtaaagg ctataaggcc tataacacca tgagagactc catcacatat aatgggacaa
     5401 cacacctctg caaactctac acaagcccca cttttatagc attccctaca tatatcacta
     5461 ctctttgggt catcaattag tattgtaatc tgctctgtaa gtgatttttt aaaagccgta
     5521 tatttatcta ctttctcacc tattttgtct atatatactc ctgtacctgc aatcctaaat
     5581 aactccttat ttactatagt tacatctata ttgagtactg atactatagc ttcagctata
     5641 ttttgaatat cagattttat atattctaaa cctatcataa tcatcattcc ttatttttct
     5701 ttaacataag tatttgtctt atttctttaa gaatcaagta tatcttattt aaagtaaatt
     5761 ggattgacat caggattaca aagagcataa caattattat taactcataa tttatttcca
     5821 tctaaacatc tccttataac aaaagactag gcttaaccta gtcttttata ttatatccat
     5881 atttatattt taactcattt atcttctttt tcccacataa atatatttta atttatttga
     5941 ctctttaatc ataccatcaa agtttaatct tgaaaatata ttaaatactt catgtggata
     6001 gtgaatcttt gcaacagaac cacaaaattt atgtttaaga tagtttaact taacttttaa
     6061 attctttaaa tttaaatcct ctaatataac ctctccattg ccctttaaca ctctttgtat
     6121 ttcagatgaa gctttttcac aatctgtaat aacattaaat tggtcaacta ttattatctt
     6181 gttgaactga ttatcatcaa atggtagaaa ctctaactta cctttaacta cattgcaatt
     6241 ttcattatac actagagaat aacagacatc ttcataatta ctattctcca gtatagttac
     6301 agaagttcca agacctctca atctcttgcc taggtttcct atatgaccaa caagcaatat
     6361 tttatcacct acattatact caattgtatc caaaaaacat tctaattcat cagatgtata
     6421 tttagcttta accggctgtt tatttatctg acataatatg cttttcacaa tgcttcacct
     6481 cttcatttcc tcaattataa attatgtaca gattacatta taataaatgg cgagcaacta
     6541 ttcaagagat atttcattag tattttttta aacaatcttt atacatttta atatgatatt
     6601 ataccaaatt tattaggatt ttattcataa aaatcagtct attttttatg tttattcatt
     6661 gttattttca tccaattttt ttattttcga tatggatact tcatatgcca tttttttgac
     6721 cacattattt tcatctattt ttttctcata ttctctactt tgaaccctac cccaaagctg
     6781 tattctatca ccaaccttta gattctttgc aaatttagca tttctacccc aaactattga
     6841 tggtatataa tcagatttat tataaggtct gtttatagca actaataaat cgcttatttc
     6901 ccttcctaaa ggtgtcttcc tatacacagg ttccttacat acatatccat ctaaaaatat
     6961 actattaggg tctttatttt cctcgtctat gctttttatt tctctgacaa acactgtaag
     7021 cactaatcta tttttattat ttaatgtttt gttatatgat cttaattggc ctacgacatt
     7081 gactaaattt tgtttatcaa ggtcaatatc ttggagaagt ctttctgata cagtcattgg
     7141 aagtatatca aaagaatcgc ttagtctgtt tatttttatt ttcatattat aaaatttttc
     7201 tccaaatatc tcatggctaa attctagctt attgtctaac tcccctctta agtttactac
     7261 gttattctca tctttaacag ctatcatatt ttatccctcc acaattgtta attcttatat
     7321 ttctggtatc agcttatata ataaatatat ttataaggga tgtattttat tacaaatctt
     7381 tgattgctgt ttaaaagttt tt
//
LOCUS       DACXMZ010000195         7342 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.196, whole genome shotgun sequence.
ACCESSION   DACXMZ010000195 DACXMZ010000000
VERSION     DACXMZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.196"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            635..1144
                     /locus_tag="JD016_15655"
     CDS             635..1144
                     /locus_tag="JD016_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAU5001058.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            1172..1366
                     /gene="rpmI"
                     /locus_tag="JD016_15660"
     CDS             1172..1366
                     /gene="rpmI"
                     /locus_tag="JD016_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAU5001059.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1422..1778
                     /gene="rplT"
                     /locus_tag="JD016_15665"
     CDS             1422..1778
                     /gene="rplT"
                     /locus_tag="JD016_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAU5001060.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            2103..2633
                     /locus_tag="JD016_15670"
     CDS             2103..2633
                     /locus_tag="JD016_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001061.1"
                     /translation="MDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATLYRYLN
                     KMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQYMMGFL
                     REFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHEEGRRM
                     HSIGVIITPPKKDEGK"
     gene            2635..2726
                     /locus_tag="JD016_15675"
                     /pseudo
     CDS             2635..2726
                     /locus_tag="JD016_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
     gene            3068..4993
                     /locus_tag="JD016_15680"
     CDS             3068..4993
                     /locus_tag="JD016_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide phosphodiesterase"
                     /protein_id="HAU5001062.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            5394..7298
                     /locus_tag="JD016_15685"
     CDS             5394..7298
                     /locus_tag="JD016_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide phosphodiesterase"
                     /protein_id="HAU5001063.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3095 a          722 c         1382 g         2143 t
ORIGIN      
        1 agcatgtatt gattaagtat tgaataaaat attacaaatt tagttgaaat gaaaatgata
       61 aaaaatcttg tactatattt tacaaatcat ataaatagag aaattgttgt agcacaaagt
      121 agtaggtagt atattagaat ataatatagg atttgttgtg tagtatagta gcatattaga
      181 atataatata ggatttgtta tgtagtatag tagtatatta gaatacaata tagaatttgt
      241 tgggtagtat agtgttatat tagaatacaa tataggattt gttatgtaga atatatagag
      301 tcatgtagaa tataaatata taaaaggatt ttgggtataa atttaaaaat taatagcaaa
      361 atgagtagta tagacatata attagtagta ggaagtaatt aaatttttcc taaacaaaaa
      421 aaatagttgc aaataatttg attaagtgat atagtagata aggtaagaat aaaacaaaga
      481 agaagtccgc ttctcacctt acgccggagg ttagtaaggt aaatataagt taaagcttaa
      541 ttttaattta agtttgttta aatttggtaa cttatattgc ggatgattac gtcatccgct
      601 ttttatttta taaaaatttt ggaggtgtcc tacaattagc aaagaattag caatcaatga
      661 acaaataaaa gataaggaaa taagagtcct ttcaccaaca ggtgaacaac ttggagtaat
      721 gcctactaaa gaagctcaag caatggctaa tagcaaaaac ttagatttgg ttcaaatctc
      781 gccgaatgca aatccaccag tatgtaagat aatggattat ggaaagttta gatatgagca
      841 agcgagaaaa gagaaagaag ctaagaaaaa acaaaaaact atagttgtaa aagaagtaag
      901 acttagacca ggtattgaac aaaatgattt aaatacgaaa gctaataatg ctataaagtt
      961 ccttaaaaaa ggggataaag tcaaagttga acttagattt agaggaagag aattaggaca
     1021 taaagacata ggtaaagaag ttatgcttaa gtttttagat ataataaaag agtttggaga
     1081 accaacaaaa gcccctgcat ttgagggtaa taatatggtt gtaattatag atccaaaaaa
     1141 atagatgatt aactgagagg aggaatttgt catgcctaaa atgaaaactc atagaggtgc
     1201 agcaaagaga ttaaagaaaa caggaactgg aaagttaaag agagctaaag cttttaaaaa
     1261 acatatatta actaaaaaat ctgcaaaaac taaaatgaac ttaagaaaat caactttagt
     1321 aagtgatgga gatgctaaga gaatagcaca attattacca tactaagata cagattgtaa
     1381 ataataacta tagaagaggg atataaaagg aggtcataac gatggcaaga gttaaaaaag
     1441 ctatgaacgc tcgtaaaaag cataagaaaa tattaaaact tgcaaaagga ttcagaggat
     1501 ctagaagcaa attatataga ccagcaaata cattcgtaat gaaagcatta aaaaatgctt
     1561 atataggaag aaagttaaag aagagagact tcagaaaact ttggatacaa agaataaatg
     1621 cagctgctag aatgaatgga atatcttatt caagattaat gaatggatta aaattatctg
     1681 gtgttgaagt taacagaaaa atgttatctg aaatggctat acaagatcca gaaggatttg
     1741 caaaattagc agaagttgct aaagcaaaat tagcttagta ttgaatcatg agatgtaaag
     1801 tctttgagtt atataaataa ttcaaggact tttttatttt ttgaaaaata cttattagtg
     1861 tcatgtacaa ccaattttat aatgataaat aaaataagca atacaataga attttatgag
     1921 atttattata agaattgtta caattattta tgatagctat aaaaagtcat agtaaaaatg
     1981 atattgatgt atgttgatat atgtataaag tttccaatca atcttgaatt aatattatca
     2041 acatgataat attatcttat taataatata aataaggggg acataatatg aatgaaaaac
     2101 taatggattg ttttgtaaac ccaattaaat gcaagttatt gattgaaatt ttttcaaata
     2161 atcgaactac agctaaacaa ttaggagaaa aatttcaaga tatatcaaag gcaacacttt
     2221 atcgttacct taataaaatg ttgaaagatg atattataaa agtggttgaa gaaaataaaa
     2281 taaggggaac tgttgaaaaa gtatatgcta tgaagttaga tatagctgat gatgtgaaca
     2341 aaattataga tgagaactct ggagaagaat atgctcaact gtttactcaa tatatgatgg
     2401 gatttttaag agaattttat gaatacgctg aaagaaagga aaaggatatc ctaaaagatg
     2461 gttcaggatt ttctgtctgt ccagtatatg ctagtattga tgagatagtt gaagtgagta
     2521 aaaaaattgc tagtatacta gaacctctaa gaaataactc acatgaagaa ggtagaagaa
     2581 tgcattctat aggagtgata attactccac ctaaaaagga tgaagggaaa taaatctgat
     2641 ttacatgggg tagagccaat ttatacaggg aatcgtttta tgatttacat atgtatccag
     2701 agcaaagtat ctctgcttgg attgtacttc attgtaccat ttcatccaat gacttggagt
     2761 ggtacaatga agttttttaa tttttcatat taatgctaaa gaaatgaact ttagaccaca
     2821 tatgcaatta ggttgcatta tacaattaga ccacatatac aaaataaaaa tcagtatttt
     2881 tatagcagat aaattgactg ttattcttct taaaaatcat ctattagtta ttgttagcat
     2941 attgaaagaa attatagtaa tctattgaat aaatgaatat tgcccataat catataaact
     3001 gctatagttt aaaataaagc aggacgttaa attttatagt aatctaaaaa tttaagaggg
     3061 ggattttatg aaaataaaat ttgttattat gccattggtg atatttttaa tggtattatt
     3121 tgtggggtgt caaagtacaa aagatacaga agttaaagca aaagatacag aaattaaaat
     3181 attaggtaca ggtgatttgc attcaatttt gacagactct atggtttcgt atgtaaatga
     3241 ggaaagggaa aagaataaaa atctgcttat ggtagatgcg ggagatttct atggcacaca
     3301 aagtagagaa atgtgggaat ggtcttctgg aaaaaagtta attaatatta aaagaaatgg
     3361 tagagcagag tatgatgaca taattaagag tagtaaagat gaagtaccta tagttaaaga
     3421 tatggcaaag ttgaaatatg atgctgtagt tttgggagat aatgaatttg tttcaaatga
     3481 taagcaaagt cttgataaac tagtttctga ttttaaaaat aataatatgc cattggtatc
     3541 agcaaatata tatgaacaat ctggagaaaa ttatgtacaa ccatatgtaa tgaaaaatat
     3601 taagacagat gagggcaatg taaaagttgg aatactaggt cttactataa aagaagtagg
     3661 ggaaaccctt ggtcttgagg attttgaaaa ggataaaaaa gctagagagc ttggagaaca
     3721 agctgattac aaaggaaaat tatatgcaaa tgatttagta gaagatgcaa aaaaatggat
     3781 aaaagttatg gaaaaagaaa gtccagacat aatagttgca gttgtacact caggggaaga
     3841 acctaaaaca gatagaaatc caggaaatag aataaaagag ttagcaacta cagtggatgg
     3901 aatagatgca attgtagctg gtcatactca tgaaaaaata gatgaacaca aatataaaaa
     3961 taaatctgga gatgaagtaa ttgttacaca accaggttgg catggagata gagtatcaga
     4021 gattaatttc aaattaaata aagagaatgg aaagtgggac ataaaggaaa agtctagtaa
     4081 attaaaaata attgataaag aagtaagcat agttgctact gcagatttac attcacattt
     4141 ctctgataaa ttaacagttg accttgttaa tgaacgtagt aacccaattg aacctgttgt
     4201 agtagatgca ggcgacttct tagaccctca aacagatgaa atgactcaat ggtataaaga
     4261 gtggaaaagt ataagagata ataatagtga taaaacgatt agtagatgcc caatggtcat
     4321 ggatatgaat cagggaatgt atgatgcagt agtacttgga aatcatgaat ttgtatctga
     4381 aaaagatgaa ttttggtcag atgaacgcct tttagatgct gttatagagg attttgaaca
     4441 ggtggcaatt tctgtgttat cggcaaatat atataggcaa tctggtaaaa attatgttaa
     4501 accatatatt ataaaagatg tggagacgag tgaaggtaat gtaaaagtag gaatattggg
     4561 acttactata aaagaagtag gtaaaggtct taaaaatgta aacttacaag agcaatttca
     4621 atataaggat aaattgtatg caaatgattt agtggaagat gcaaagaaat gggtaaaagt
     4681 tatgaaaaag aaaaaaccag atataatagt tgcagttgta cattcaggag aagaaccaaa
     4741 aaatccaaaa catccaggaa atagaataaa agaactagca actacagtag atggaataga
     4801 tgcaattgtt gctagtcata ctcatgaaaa aatagatgag catgaatata aaaataagtc
     4861 tggagaaaaa gttatagtga cacaaccagg tgagcatggt aaatattatt ctaaaattac
     4921 ctttaaagta aataaagaaa agggttcgtg gattattaat gataaattca gtaaaacaat
     4981 tgaggttgaa taactttata aaatttatag ataaagtaaa ttatctgata ttgacatgat
     5041 atataagata aagtttctaa tattagtata atgcataatg tgaaatattt taataataag
     5101 agaataaaca agacaaataa aaatatttta acatgtaaga tatagtatga actattatac
     5161 taatgcttac tttataattt gaacatatta gaataagtct tatttgaaaa ttttagtgta
     5221 ttagagtaag tattatttta ccagtaaaaa atattgagaa ctatatgtag atttgcttaa
     5281 aaaggaattt acatatagtt cttttcgtat aataaatagt acaatcttat atggtaaaga
     5341 taaagtgtga tattaaattt tattatcatc caaaatctaa ggaggagtct cttatgaaaa
     5401 taaaatttat tatagtagca tcaataatat ttttaatagt attacttgtg gggtgtcaaa
     5461 gtactaaaga cacagaaatc aatgtactcg ccacaagtga tttacattca attattccag
     5521 ataatttaat ttcctatgta gaagaggaga gaaaatatga tgagaatcta cttatggtag
     5581 atgcaggaga tttttttgac atgcaaagtg gtgagatgaa taagtggttt actggtcaaa
     5641 aattagtaag atttaaagat gggataccag agtacaaaaa aatagctgaa ccacgtgaag
     5701 gagaagttcc attggctaaa aaaatggcaa agctgaagta tgatgcagtt acatttggga
     5761 atcatgaatt tgtatctaat gacaagcaga gccttgataa attggtttct gattttaaaa
     5821 ataacaatat accattagta tcagcaaacg tatatgaaca atctggagaa aactacgtaa
     5881 agccatatgt aatgaaaaat ataaatactg acaagggaac tgtaaaggtt ggtatacttg
     5941 gtctgactat aaaagaagta ggggaacgtt caaggtttaa aagttttgaa caagataaaa
     6001 aggctagaga acttggggaa caacctcaat acaaagggaa attatatgca aatgatttag
     6061 tagaagatgc aaataagtgg gtaaaggtta tggaaaaaaa agaaaagcca gatataatag
     6121 ttgccattgt acactcaggt gaagagccaa aaaatcctaa aaatccagga aatagaataa
     6181 aagaactagc aacaactgta gagggaatag atgctattgt agcaggtcat acacatgaaa
     6241 aaatagaaca gcatacatat aaaaataatt ctggtgaaga agtaatagta acacagcctg
     6301 gagctcatgg taatagtata tcaaagatta attttaagtt ggataagaag aatgataagt
     6361 ggtttataaa agacaaatat agtgaattaa aggtttttgg aaaggaagta aatataatta
     6421 caacttctgg tctgaatcca aaattttcag atgaaatggc agttaatttg tttaatgaaa
     6481 gagatagaga tgttcaacct atctttatag atactggaga ttttttaaat actaatacaa
     6541 aagaaatgac taagtgggct aaagagtggc aatacaatga aaataaaggc atctataaaa
     6601 aaataaatga atggccaatt gtatatcttg gttatgatgc tgtagtactt ggtagccatg
     6661 aatttacaga ggataaagag acttttgagg aaaaaaaatc tacattggat tatattatag
     6721 aaaactttga agaaatgaag ataccagtat tatcagctaa tatatatgat gaatctggag
     6781 aaaattatgt aaaaccttat ataattcata atgtagaaac tagggaagga aatataaagg
     6841 ttggagtatt aggtcttact actactgata aggatgaaga agacttaagt aactcaagct
     6901 taagcaaaga ctcaaagact tcgaaaattc ctgaagaaac aggaaagtta tatactaata
     6961 atctagtaaa agatgcaagc gaatgggtaa aagttatgca aaaggaaagt cctgatgtga
     7021 ttgttgcagt tgtacattca gataataata gtactctaaa aaatccaagt agtgaagtca
     7081 aaaaacttgc aatgtcagta gatggaatag atgctattgt tgtaggtcat acgaaaaata
     7141 aggtagaaga acatatttac aaaaataagt ctggggatga ggttatagta acacaatctg
     7201 gagaagattg ttattcaaaa ataagtcttg aactaagaaa agaaaatggc aagtggaata
     7261 tcataaacaa atatagtaaa gcaataaaaa tgaagtgaat ttattgatat ataaaccatt
     7321 attaatactg atttttataa ta
//
LOCUS       DACXMZ010000196         7335 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.197, whole genome shotgun sequence.
ACCESSION   DACXMZ010000196 DACXMZ010000000
VERSION     DACXMZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.197"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(11..709)
                     /gene="yunB"
                     /locus_tag="JD016_15690"
     CDS             complement(11..709)
                     /gene="yunB"
                     /locus_tag="JD016_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HAU5001064.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            923..3532
                     /locus_tag="JD016_15695"
     CDS             923..3532
                     /locus_tag="JD016_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAU5001065.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVEPEVAYVVTDMLESVITEGTGGVAALPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSIST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDGGGNNGGG
                     STTPPDGGGTPAT"
     gene            complement(3807..4742)
                     /locus_tag="JD016_15700"
     CDS             complement(3807..4742)
                     /locus_tag="JD016_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HAU5001066.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(4786..5931)
                     /locus_tag="JD016_15705"
     CDS             complement(4786..5931)
                     /locus_tag="JD016_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAU5001067.1"
                     /translation="MNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPELAFEEYKSQ
                     KALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPGMGHACGHNI
                     IGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFNNVDAALILH
                     PSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSVNSMRLHLKD
                     YARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKGAAISTGTNV
                     EIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGNLTHEIPAIQ
                     AYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            complement(5945..6364)
                     /locus_tag="JD016_15710"
     CDS             complement(5945..6364)
                     /locus_tag="JD016_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001068.1"
                     /translation="MFIYIIICFGQMIALKTQAQDLLIGSVSGFLLVIIAIIIKNFVK
                     KPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTPLLAFAGISVGNQLDI
                     LKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            complement(6424..7266)
                     /locus_tag="JD016_15715"
     CDS             complement(6424..7266)
                     /locus_tag="JD016_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HAU5001069.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
BASE COUNT         2483 a         1273 c         1042 g         2537 t
ORIGIN      
        1 caactttata ttacttgttg gtattctttc cagtgttagg taaaacattt cctagatttt
       61 tttctggaat atttacataa ctttctggaa catcacctac tattatagtt tcacaaatag
      121 gtatttgagc tttaatttgt tttgttgaag ttataagtgg tattactact ttaacttgtg
      181 tttgagcttc taaatatatt ctatgtctag tttgatttat acctgaagac tcaaattccg
      241 ttttaaagtt tactgatact gttcctatag gttcaataga tacttcaagt ttaggaccgt
      301 atttagctaa tataggactt cctaatgctg ttcctatagg tatgtaagaa cttgctgtct
      361 tatccttttt taattcatct tgtatttcta aagctattgc agatgcaatt tcattcatca
      421 ttatagtgtt tgcttgtatc atagttattt taccattatt atcaagttgt atatccatta
      481 aatcttcata atttattttt ccttctacca tctcagcaac agctttattt atcgacctat
      541 ttgctagttc aagagcttta gtctctgcta aaactgttat tgtcggtctt aaagttttat
      601 ctatatatat aaaactgcct ataaatacag acaagaaaag aattactaaa aaaatagcta
      661 tgatttttct aaattcattt tttgtttttc tatctactat tctattcaaa attatcccct
      721 cctatttatt tatatgtagc tttggcataa tttttataat atttgtcaaa aataaataaa
      781 ataaaaatat agacttttta atatattacg tttatacata tacaatgtac agaatattat
      841 agcaaattca ttttttctat taattacaaa actactccta tttgtgttat aatattgcat
      901 taagtaagga ggttaaaatc ttatgaataa cgataatgat aaaaatggaa ataaaatcag
      961 gagaaaaaaa gttagctcat ctggtaatac aaataaacca attaatagaa cttcagctaa
     1021 caagactcgt tcaacaaaaa acaagaaaaa atctaaaaaa agcgataaat ttaaaaaact
     1081 aagagtattt gggatagtat ttttagtttt actagttgtt ggtacagcag gtactgctgg
     1141 tctagtattt gcgtcactaa gagatgtaac tccagttaca gaagctgttt tagataaaca
     1201 gacgaaccaa acaactacta ttaaatacgc taatggaaaa acattatcta cagctccaag
     1261 tgtaaataaa aagacacccg ttccattaga taagatttcc ccttatcttc aacatgctgt
     1321 aattgcaata gaagatgagc gtttttatga gcataagggt gtcgatatta aaggtttatt
     1381 taggtcagta ttaaagaccc ttacaggtac taagcaaggt ggtagtacta ttcctatgca
     1441 ggtatccaaa atgctgttaa caactgaaca gcaaactata cctcgtaaaa taaaggatat
     1501 ttactatgca catgagatga gtaaaacagt aagtaaagat aaaatactag aaacttattt
     1561 aaataacttc ttcgttggta gaggtctagc aggtgctgag gctggtgcac gtggttattt
     1621 tgataagtct gcagctgacc ttactctagc agagtcagca cttcttgctg gttcaactaa
     1681 aaatccatca agattttcag cctataaaac ttctaagtta gagggaaatg aaactaagga
     1741 ggatttagag aataaattat tattttttgt taacacaact gatgatgatt tagatgaccc
     1801 tactcaagtg gactttgata tgattgaaaa aataaaatct tgggagctta tatctaatga
     1861 tacttataga caattaaaag caggtacatt agttgttaga aaggctgtaa gtaatcctga
     1921 agctaaaaaa agacaagaaa ttgttttaaa gaaaatgtta gaattgaaat atataaaaca
     1981 aagtgaatat gatgaagcaa tcaaagctaa aatagaaata aaattgcctc aatcatcaga
     2041 taaagtttct tcatctgtag aagaccttat cgaaagtgaa gttataaatg ctttaatgga
     2101 acaaggacat acaaatgatg aagctcaaaa cttattctac aatggtggtc taattgtaaa
     2161 cacaactata gaccctaaaa tgcaagatgc tttagaagaa gaatttgata gaaatagcaa
     2221 tttcccagga catatggtcg gtcctgatgg agttagccaa ccgcaagctg caatggtaat
     2281 cttagattat aaaaatggtg aaataagagc actagctgga ggaagaaata taagtggtag
     2341 aaaaacttta aatcgtgcta ctaaccctca tcagccaggt tcttctataa aacctttagc
     2401 aatatatacg ccagctattg atactctaaa aataacacaa gcaactgctc taagtgattc
     2461 tcgaggagga tataagtttg aagaaaataa taaatggaat ccaagaacta ctactgctgg
     2521 tcatggttct atgagtttac gtaaagcact tgctaaatct tcaaacacga tagctgtcaa
     2581 aactgctgaa atgcttggag actcttatga tgaatgtgta gatataatga tggattactt
     2641 aaagaacttt ggtataacaa ctttaaagaa taatcactca ggttcatctg aagcaagtga
     2701 tagaaaattc ccatcattga cactaggtgg tatggcgaat ggtattactc cacttcaaat
     2761 ggctgctgct tatggtacat tggcaaatca cggtatttat gtagaacctt ctatttttac
     2821 tactataact acctttgatg gtcaattatt agttaaaaat gctcctgagg aacataaagt
     2881 tgtagaacct gaagtagctt atgtcgttac agatatgtta gaaagtgtaa ttactgaagg
     2941 tactggtggt gttgctgcac ttccaaaggg aatgcctgta gcaggtaaaa caggtactac
     3001 aaatagtgca tatgatgctt ggtttgttgg ttacactcct tattatgttg gtgcaactta
     3061 tattggagat gatgctggta gaaaagatga ttctggtaac actataaaac gtagagaagt
     3121 accacatgga agtattagta ctgcaaaact atgggaaaaa attatggaaa aaatacatgc
     3181 taatttaacg gttactgaat ttgaagttcc taaaaatgta tattttacaa aaattaattt
     3241 agaggatgga ggaaagcaat cttctggctc taaagcagca tttattgaag gtactgctcc
     3301 tactaaagta agttctcaac catcatctga agatacaaaa aaaccagaca ataaccaacc
     3361 agaagaaaat aataataaca ataataacac aggtgataat ggtggcggtt ctacacctcc
     3421 tgataatggc ggaaacaatg gtggtggctc tactactcct cctgatggtg gtggaaataa
     3481 tggtggtggc tctactactc ctcctgatgg tggtggaact ccagcaactt aataaaaaat
     3541 agaatttaaa taaaaatata aggagttgtc tcaaggtgat taaaaaaatc actttgaaac
     3601 gctccttttt ttataattac aatatattat ttgtattcat aaaaaagaga atcccaataa
     3661 ccagaatcct ccttttttat ataaacaaat aaatatttta tttttaaagt ttttaactaa
     3721 gtgctttatt cttactttta ttcctaattg tttatatctt cttaaatagt tgtatcacaa
     3781 attatataaa cttaatattt ttctaattac tgtataccaa atgtttgaaa tctatttaat
     3841 ttgccatatc tcttaataag catttcccac tcttctttat catagaaatc acacttattt
     3901 ctaccaaata gcttggcaac ttcaattgca aatctcccag ctgaatctaa atcactaaaa
     3961 ttagttgcac ctgtagcaca tccagcaaca ggcatctctg ttgtaattgc tactcctact
     4021 attggtgcat ctgtagctac tgctggttgt aaaatactat ttaagtgata cagattattt
     4081 ccatatggtg ttatatcttg aatagacaaa gggaatacaa atggagattt ccctgttaca
     4141 gtctgcataa tatctaaaag gttatcacta gtttttaata tgtagccttc tttaacagtt
     4201 ggcgatattg caaatcctct agtattgatt actctatttc ctttagtagt atctatagaa
     4261 agtattgcat cacaactact atcaacttca ttttcattca tagtcatcat atcaattgga
     4321 gaatccataa aaggagtagg atagtgttct ttagttggag catctgggca aatatgggtt
     4381 gaaattacaa catcaccatc taatatatcc ccttttcgtt gcatatccaa aagtttagca
     4441 gccactgcaa ttgctaccaa tgctccatca ccatctgaaa caaaacctat aacttctggt
     4501 cttgctccaa tccctcctag tctacctaaa atgccaagag taggagcatc tccacctttt
     4561 aattttccat ttttcccagg aattgtaagc tttactaaat ctgttgagcc tttacttgaa
     4621 gacaattctt ttacagttac atctacttca ccataacctt gtaagtaagt ttttacttct
     4681 tccccattag catttgcttt atctaaaatg tcataaacct ctattacttg ttttaatatc
     4741 atgtctaatc tgatttcctt tcttaatata ttttaaaatt atatattatt tactatacaa
     4801 aatatctact ccacacattg ctagcacctt aattgctttt ataagtgcta ctttgccttc
     4861 ttcaccacca gctgcaacag caaattcatc tgtatgagtt gcagtatttt cttttaattt
     4921 tatatatgct tgtattgcag gtatctcatg agttaaattt cctgtatcag ttgaacctat
     4981 accttgactt aaatctcttt caacatagtc ttcacccaaa acttcaaaat tttgtctaac
     5041 tatattcact aattcagaat cattcttaat ctctttgtat atttcatcta attgaactat
     5101 ttctacatta gttccagtag aaattgcagc tcctttagca caatttttaa gcatttcaca
     5161 aatctcattt aaatattcta tgcttaaagc tcttacatta aatatagctg tagatttttc
     5221 gggaattata ttatgtatgc tacctccatc agttataatt ccatgtactc ttgcataatc
     5281 tttaagatgt aatctcatgc tatttacact gttaaatagt gctattactc cacttaaggc
     5341 acttttccct tcccaaggaa aagcagcagc atgtgatgcc ttaccagtaa aatcaaattt
     5401 taaattaact tgagcaaatg atatatcatc aggcattgaa gcatcacttg gatgtaaaat
     5461 taatgcagca tctacattat taaatacacc ttctttaatc atagttattt ttccacctac
     5521 tctttcttct gcaggtgttc caaaaacctt tacagtccct tctatattat gttttttcat
     5581 gatttctttt aatatgatac ctgcaccaac agaacttgtc cctatgatat tgtgcccaca
     5641 tgcatgtccc attcctggaa gtgcatcata ttcagctaaa aaagcaactg ttttcccatt
     5701 agttccattt gaatacacag cttcaaaaga tgtttctaat cctcctacac ctttagttac
     5761 attaaattca ttttcttcta acaaatcaca taatgctttt tgtgacttat actcctcaaa
     5821 tgctaattct ggatttgaaa ataaatagtc agaaactttt tcaaatgatg tctttttctc
     5881 ttcagataat aactttattt cctctttaat ttttgctttt aattcattca tatttactac
     5941 ctccttaaag cattccattt aatcttaaaa ctgtttgaga tataaaagca gaaccaaagt
     6001 aagtagatgt cataactata aatgatataa ctactaactt ccaactcaaa gtctttaata
     6061 tatctaattg gttacctaca gagattcctg caaaagctaa taatggtgtt actgtagaca
     6121 taaaatttat tttccctaca tttgttgcta tgatatcccc cactggagat attggaagcg
     6181 ataaaacgaa tgcaactaat gtagcccatg caaaacctgg taaattaggt tttttaacaa
     6241 aattctttat tataatagca attatcacca acaaaaatcc agatacactt ccaatcaaca
     6301 aatcttgagc ctgagtctta agtgctatca tttgtccaaa gcaaataata atatatataa
     6361 acaatactat ttttatctgc tccataatag tcttaaaagt tttttccata tatatatcct
     6421 cctttacaat ttattacttg ttcttcttgt ttttcttcct ctaaatcttt caataactgg
     6481 agctactttg ctataatagt agttggctac tggtaaagat aagaataatt ctatatatat
     6541 tccagtaaca gctgtaatca atccactagt agctgaatat gctaaaatct gctctgacat
     6601 ttgtggaacc gtattcatta atgatgctgt tgcagctccc atcattgaac cactacccat
     6661 tcctgatgcc attgctaaag catatggatg aaatcctaaa agtacaccca ctggtgctaa
     6721 aaatgaaaat aataatgttc caaatatact accacttatg taagttccta gtgttcctat
     6781 accttctggt gagtttaatc catacttatc tccaataatt cctagtgctg tgtcacgaca
     6841 taatgaaaaa gtaccaccta atgcacttct tccaagaccc aatgctactc caacaggtaa
     6901 tgctatcaac attgtaccca tatgaccaaa ttcttgtaat aaaagtgctg gtcctgcttg
     6961 aagtaatata ccaactttag gccctgcatt aattccaaac attatcccta atggatatag
     7021 tgtcaaagat aataaatatg gagatacatt tatttcatta tcccctataa acttttttaa
     7081 ggctggtata actctcccta ataaatgagg agaaacagcc attcctatga atatagccca
     7141 tagtatacta agtaaagtta cattaattcc cataacttta aatgaaatag gtccaattaa
     7201 ttctgccaat attgtgatta taaaggctgc tattatggca tttctaatcc ctgctttttc
     7261 tttcatttct atacctcctt gtaattttta taattttctt ataatataat tgttagtgat
     7321 tttattatat aacct
//
LOCUS       DACXMZ010000197         7240 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.198, whole genome shotgun sequence.
ACCESSION   DACXMZ010000197 DACXMZ010000000
VERSION     DACXMZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.198"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            129..1634
                     /locus_tag="JD016_15720"
     CDS             129..1634
                     /locus_tag="JD016_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAU5001070.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            2024..3121
                     /gene="dinB"
                     /locus_tag="JD016_15725"
     CDS             2024..3121
                     /gene="dinB"
                     /locus_tag="JD016_15725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAU5001071.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEFNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            3384..4520
                     /locus_tag="JD016_15730"
     CDS             3384..4520
                     /locus_tag="JD016_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087374.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAU5001072.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            4507..6060
                     /locus_tag="JD016_15735"
     CDS             4507..6060
                     /locus_tag="JD016_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAU5001073.1"
                     /translation="MFLANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGI
                     IISRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNT
                     YIGITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAF
                     IGAGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPK
                     KNYIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNT
                     NLKVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDT
                     MEKENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENRE
                     DGLVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDK
                     NFFVPYYAAPLVREDTLKKYPELEKVMNMFSGKINEETMRELNYQVDELGKSPEEVAH
                     SFLVKEGLV"
     gene            6394..>7240
                     /locus_tag="JD016_15740"
     CDS             6394..>7240
                     /locus_tag="JD016_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAU5001074.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHI"
BASE COUNT         2752 a          784 c         1292 g         2412 t
ORIGIN      
        1 ttaatcttaa ttgtataata ttactttgag tgaaaaagta ttaacatttt tttataagat
       61 gttttactaa aaaaatatat ataaatatac tgttatcaac aagaaattta atttagtgag
      121 gtgttaagat gaaagtagga tttgaccaca aaaaatattt agaagagcag tctaaatata
      181 tcttagaaag agtaaataat tttgacaaac tttatttaga gtttggagga aaattgatgg
      241 gagaccttca tgcaaaaaga gtacttccag gatttgatga aaatgcaaag ataaaagtat
      301 tacaacatat gaaagaaaaa gttgaagttg taatttgtgt ctatgcagga gatatagaaa
      361 gaaacaaaat aagaggagac tttggtatca cttatgatat ggaagtttta agattaatag
      421 atgatttaag aggttatgaa ctagatgtaa atagtgtagt aattactaga tttgaaggac
      481 aacctgctac aactgtattt ataaataaat tagaacgtag aggaattaaa gtttataagc
      541 attatccaac aaagggatat ccgtcagatg ttgagactat agttagtgat gagggatatg
      601 gagcaaatga atatatagaa actacaaagc caatagtagt agtcacagca ccaggaccta
      661 atagtggaaa acttggtact tgtctaagtc agttgtatca tgaaaataaa agaggtaatg
      721 tagttggata ttctaagttt gagacattcc cagtttggaa tgtacctgta aaacatcctt
      781 tgaatatagc ttatgaagca gctacagttg atttaaaaga tgtgaatatg atagattcat
      841 tccaccttga aacgtatggt gaaatgtcag taaactacaa tagagatttg gaattatttc
      901 cagtgcttaa aaaaataata gaaaagataa ctggaaaaga atcaattttc caatctccaa
      961 ctgatatggg tgttaataga gttggctttg gaataataga tgatgaagta ataagaaaag
     1021 cttctataga agaaataata agaagatact ttaaaactgc ctgtgagtat aaaaagggac
     1081 aagttgataa aggtgcttat gatagaatga aaatgattat ggaagagctt aacttaaagc
     1141 cagaagatag aaatgttgta ttacctgcta gaaactacag taataaattg aaagaaagtg
     1201 ccgacaagaa tgatacttgt cctgtagttg cattagaatt agaagatggt acaatattaa
     1261 ctggtaaggg ttctgattta atgaatggaa ctgcagcagt agtattaaat gccattaaac
     1321 atttagccaa tatatctgat gatatgcact taatatcacc tgtaatatta gaacctatca
     1381 taaacttaaa aacaaagact ttagctagta gaaatgtttc acttagttgc caagaggtgt
     1441 taactgcact tagtatttgt gctgtaacta atccaactgc acaatttgct atggaaaagt
     1501 tagcattatt aaaagggtgt caagctcatt ctactactat attaaataga gatgatgaac
     1561 aaacatttag aaaacttggt atagatgtaa cttgtgaccc agaatatcct tcacaaaatc
     1621 tttactatag ttaaaattat ataaaaaaat atgggctgtt ttaaagtaat agtttgagca
     1681 gtccattaca attataataa aatcagtagg ctgtcttgta taagacagtc ttttgttttt
     1741 aaaacatccc ataaattaaa tatatatact gttatccata aggaatttta aatttagttc
     1801 cttggaatac ctattgtaaa gcttttatat agtttttgca agtgttttta aggatttttt
     1861 ataaattata ttgaagttaa tggattatag atggttttat ttatggaaaa tatgaatata
     1921 taaagaaaat aatgaaaaaa taccaaaaag aaacataagt ttggtataat taaaaagtaa
     1981 tataaataaa attaatctat ttatacttta aaaggtgata ataatgaaac attatagaaa
     2041 aataattcat atagatatgg acgcttttta tgcatctata gaacaaagag ataataaaaa
     2101 attaaaagga agacctgtta ttgtcggtgg aaatccacaa agccgaggtg tggttgcaac
     2161 gtgttcttat gaagctagaa aatttggtat ccattcagct atgccatcag ctgtagctta
     2221 caataggtgt ccatatgcag tttttgtaag acctagattt gatgtttata agagtgtatc
     2281 agagcaaata agagatatat tttatagata cacggattta gttgaaccat tatctttaga
     2341 tgaagcttac ttagatgtaa caaaaaataa aaaaaatatt gattcatcaa ttgagatagc
     2401 aaaacaaata aaaaaagata tatttagaga agttggtctt acttcttcag ctggtgtgtc
     2461 atacaataag tttcttgcta agattgcatc agacttaaga aagccaaatg ggttaactgt
     2521 aataaccgaa gaaaatgccc aggacttttt agataaatta cctgtgaata aattttttgg
     2581 tgttggaaaa gttacttcaa atactcttaa aaatttaggt ataaaaactg gatatgattt
     2641 aaggtgttta aatttatttg aacttgaaaa tatattcaaa aaaagaggtt atgaattata
     2701 taaatttgct agaggaatag acgatagacc agtggaacca aatagggtta gaaaatctgt
     2761 tggggctgag actacattaa gtcataattt ggatattgat gaagaagaaa cacgcaacat
     2821 tcttgatgaa ctttgtgaag aagtatgtca tagattaaaa aactctgaga agtttggaaa
     2881 aacattaaca ttaaaaatta aatatgagga ctttacaaag ataactagaa gccttagttt
     2941 agaacactat attgacgaat ataatgatat aagaagtggt gtagataatt tgttgagaaa
     3001 tgttgagttt aatggaaaac aaattaggct attgggagtt acaatatcaa acctaagtga
     3061 taaaaaagaa acttataaag atattacatt atttgaatac atggatagta tccaaatgtg
     3121 agttttgttt aaatgaaata acactagctt taatactata taattgaggc tagtgttatt
     3181 ttatgaatat ttttatattt tttgtttttt gacattatat attatatact atatataaat
     3241 actttatatt tgtagcaata ttcagacaaa tagtgaccta aaaatcgtac tttgtttaca
     3301 taaataaaat agtatgatat aattataaaa acaacttata atattgaaga aggttgcttg
     3361 ttcgaaaaag gggatgataa attatgatag agataagaaa tgtaacaaaa aaaataggaa
     3421 ataatgttat attagatgat atttctctag ttgtagaaac aggtacattg gtagttttga
     3481 ttggttcaag tggttgtgga aagactacta ctttgaagct tataaacaaa ttaataaaac
     3541 ctacatctgg tgagatatat attaatggca aaccaatttc tcaggaaaat gaaatagagc
     3601 ttagaagaaa aataggatat gttatacaga acacaggcct attccctcat cttacaataa
     3661 aagaaaatat agagctaatt ccacgcctaa aaaaggaaaa atccgttgaa gaaattgaaa
     3721 aaagaacgtt gcaactactt gagatggtag gacttgatag tgatgaattt ttgaataagt
     3781 atccttcaga acttagtggt ggacagcaac aaagaatcgg tgttgcaaga gccattgcta
     3841 ctgatgctga aataatattg atggatgagc cttttagtgc tttagaccca ataacgagaa
     3901 catctttaca agaacaatta ttttctcttc aagatgaatt gaaaaaaact ataatatttg
     3961 taacgcatga tatggatgag gctttaaaga tagctgataa aatttgtatt atgaaggatg
     4021 gaagaatagc acaatatgat actcccgaaa atatacttag aaagcctgca aatgactttg
     4081 taaaagattt tatcggagaa gatagagttt gggataatcc agaatatata aaagcaagag
     4141 atattatgat aaaaaatcct attgctgtta attctactag aactgtaact caaggaatag
     4201 aaattatgag aacgagtaag gttgatagct tactaattat agatagagca aaaacattaa
     4261 aaggtattgt tactgtaaaa gatatgaaag atattgatga taagtcaata ttacttgctg
     4321 atattatgtc aagtgagcct ttgcatgtaa atgaaggtga taatttagta gaaatactaa
     4381 atgtaatgaa tcgtaattcg gttggatata ttccggttat ttctgatgaa aataaactgg
     4441 tagggcttat tactagaagt agtttacttt ctgttttaag tgaacaattc ttagaaatgg
     4501 aggtgagtgt tcttggctaa tttttttaac tttatactat tacaaaaaga taagattata
     4561 gagcttttaa tacaacatat gagtctaact gttacatcta ttttaatagc tataatagtt
     4621 ggtgttcctc ttggaataat tatttcaaga atctcatctc ttagaaagtt tgtacttgga
     4681 tttgtaaact tagtccaagc agttccgtct atggcacttt tgggtcttct tgtacctata
     4741 cttggtatag gaagtaaacc agccatattt atggtagtgg tttattcatt actaccaatt
     4801 gtaaaaaata cgtatatagg tataacaagt attgacccag tagtattaga atcagctaaa
     4861 ggtattggtc ttacaagaaa tcagacatta tttaaaatac aattcccttt agcacttcct
     4921 ataataatgg gtggagttag aatatctgca gttacagctg ttggtttgat gactcttgca
     4981 gcatttatag gtgctggtgg tttaggatat ttagtatttt caggggttca aactgtaaat
     5041 aataatatga ttttagcagg ggcaatccca tcatgtataa ttgcactgtt agttgattat
     5101 ttatttagta aaattgaagt tgcagttact ccaaaaggat taaatccaaa agcacctaag
     5161 aagaattata tagcacttaa agtaatatct gtagtggttg ttgtttcaat gttgttcgtt
     5221 gtttttagtt ctagtttttc aagtaaaaaa gatacaataa ctattggttc taaagattat
     5281 acagaacaac ttatacttgg taatgtttat gctgaattag tagaaaaaaa tacgaatctt
     5341 aaagttaaga agaatttaaa tctaggtggt tcatctgttg cgtttaatgc aattaaatct
     5401 ggtgagttag acatgtatgt tgactataca ggtacacttt tggtaaatgt aatgaaacat
     5461 gctccgatta aagatgctga tgaagcatat aatgttgtaa aagatactat ggaaaaagaa
     5521 aatcagttaa ctttacttga ccctcttgga tttaataata cttacacatt agctatgatg
     5581 cctgaaactg ctgaaaagta tggaataaat actatttctg atttgactaa atatggtaaa
     5641 gaatttacat tctcaccaac tttggagttt gaaaatagag aagatggtct tgtagggctt
     5701 tctagagatt atggtttaaa attcaaagat gtaaaagcaa tgaatggaag tttaagatat
     5761 acagctttag ataataatga atctcaggtt atagatgcat ttttaactga tggtcttatt
     5821 aaaaagttta atcttaagat attagaggat gataaaaatt tctttgtacc atattatgca
     5881 gcaccattag taagagaaga tactttgaag aaatatcctg agttagaaaa agttatgaat
     5941 atgttttcag gaaaaataaa tgaagaaact atgagagaac ttaactatca agtggatgaa
     6001 cttggtaaat caccagaaga agttgctcat tcattcttag ttaaagaagg cttagtataa
     6061 aaaattaata gtttatatca aaaatttaag ggagtgtctc aaaatgaact ttttagttca
     6121 tgaggcactc ttttttgtat gaagtcaaat atattttatc atttcaacaa taaaaaagaa
     6181 gattatcttt attttgagac agtatcttat ttttatgtat acaaaaacca tacctacgat
     6241 ttttatttat aattaagttt caaatagaat gaacaagtac aaccaatttt gaatataaat
     6301 atagtaaatg tgcttatata cgattttatt gtctggttga ttgatattga tagatagctt
     6361 tctttattga atcgaaggga gggggaattt gttatggata actataagaa agtgaagcaa
     6421 gtattgtgga taattttatt tgctaatttt gcagtagcat tattaaaaat cattattggt
     6481 aatcaaataa aaagctatag tatgacagca gacggatttc attctttgtc agatggagca
     6541 tctaatatag tggggcttat aggtattttc tttgcatcta aaccaaaaga taaaaatcat
     6601 ccttatggac ataaaaagtt tgaaattata accagtttat ttataagtgg tatgttgttt
     6661 gttatagcaa ttaagataat tcttagtgca gtgttaagaa tagctaatcc agttgtacct
     6721 gctataacta tagaaagttt aattgcttta attattacac tttttataaa tatttttgta
     6781 tgcatgtatg aatatagaat tgggactaaa ctaaatagtt atgttttgat ttcggattca
     6841 ttgcatacaa gaagtgatat atttgtgtct ctaggtgtat tggttacttt agttggtgtt
     6901 aagttaggat ttccagtaat aatagaatct atagttccta ttattatttc tgcattcata
     6961 atttattcgg cttatggaat atttaggcca tctattggaa ttttggtcga tagagtggct
     7021 gtagatgagg attatataaa agaaattgtg tttgaattta atgaggtaag ggatgttcac
     7081 aatatcagaa gtagaggtag caaaagttct atttatattg acatgcatgt aatggtagac
     7141 ccatttataa gtgtagagca atctcatgat ttaacacata aaattgagaa acaaatacaa
     7201 gaagaaatta atgaaaatgc tcaagtaata gtccatattg
//
LOCUS       DACXMZ010000198         7231 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.199, whole genome shotgun sequence.
ACCESSION   DACXMZ010000198 DACXMZ010000000
VERSION     DACXMZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.199"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            43..1152
                     /locus_tag="JD016_15745"
     CDS             43..1152
                     /locus_tag="JD016_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAU5001075.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            1435..2826
                     /gene="hydA"
                     /locus_tag="JD016_15750"
     CDS             1435..2826
                     /gene="hydA"
                     /locus_tag="JD016_15750"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HAU5001076.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            2820..5381
                     /gene="xdh"
                     /locus_tag="JD016_15755"
     CDS             2820..5381
                     /gene="xdh"
                     /locus_tag="JD016_15755"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HAU5001077.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            5533..6879
                     /gene="rpoN"
                     /locus_tag="JD016_15760"
     CDS             5533..6879
                     /gene="rpoN"
                     /locus_tag="JD016_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAU5001078.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT         2902 a          833 c         1493 g         2003 t
ORIGIN      
        1 tctaaatatt aaaaatattt aatttaaggg agagataata atatggctaa tttaaaagtt
       61 aatttatatg ggatggaatt tttaaaccca attatgacag cggcaggacc tggagcaaaa
      121 gatggtgctc tctgtcaaga ggcagcaaaa ggtggagcag gagctatatg tacaaaaact
      181 atatctgtat taccagctga tgtaccaaga ccatgtatgg caaaaactaa tagtggtttt
      241 ttaaacacag aattatggtc agagcttcca aaagagcaat ggatagagga agaatacaaa
      301 aaggtaaaag aaacagggct tccagtaata gtaagtatgg gatattcagc agagcaaata
      361 gctgaggttg ctcctttagt aaagccttat gcagatgcag ttgaattatc tactcattat
      421 gtaggtacag acgttgcacc aatagtaaat gctttaaaag ctgcaaaagc tgttttagat
      481 gtaccagtat ttatgaaaat gagtcctcat actgacattc aaactattgc aaaagctgtt
      541 gaagaagcag gtgcagatgg acttgtaatg ataaactcat ttggaccatg tatggcaata
      601 gatgtaaata caggataccc tataatggga agtaaagctg gatacggatg gttatcaggt
      661 gcaccaataa gacctctagc aataagatgt atatatgaag cttctcaagt tgtaaatata
      721 ccaataatcg gtgttggtgg aatcacttgt ggaaaagacg tggctgaaat gatgatggct
      781 ggagcaagtg ctgttcaagt atgtacagaa gcaatcctta aagggccttc tatctatgga
      841 aaaattgcta aagaattaaa tgaattttta gatgagaatg gatatgctga tgttaatgaa
      901 ataaaaggat taactcataa gaaaatggca gctagagggt ttagaactca ttcagtagct
      961 ccagtagtgg atgataacaa gtgtatcaaa tgtaagcaat gtgtaacaag ctgtgtatac
     1021 gaagctttag aagttacaga taagttgaac atagatgcag acaagtgttt tggatgtgga
     1081 ttatgtgtaa ctagatgtcc aaaaggagct ttaagcatct gctacaatca agtagaagct
     1141 atgaatgagt aataattata tagaattttg gtaattaact tagagtaata ggtattagga
     1201 taataaaata tatcagataa acaaacacac attatgcatt aataatacag tctaaagtta
     1261 ataattttat tatattgatt taactaaata ttagtttagc tgaatattaa tttaattagg
     1321 tattggttta atcttaatat agatttactg cgtataagtg aactaactag attactttaa
     1381 gttaattttt tcatataaaa taaattgata cattacaagt aaggtggagt ctatatggga
     1441 attgttttaa aaggtgggaa agtagtaaca agtgaaaaaa cttactgtgc agatgtaaga
     1501 atagaagatg aaaaaatagt cgatataggt cttgatatat caaaagaaaa tgatgaaata
     1561 gttgatgtta gtgattctta tgttatacca ggaggtatag atacacacac acactttgac
     1621 ttagatacag gcgttactaa gacagcagat aactttgaga caggaacaaa ggctgctata
     1681 gtaggaggaa ctactactat attggatttt gctacacaag ctaaaggatg ttctttgaaa
     1741 gacggattag aagaatggca tgaaaaagct aaggataaat cctattcaga ttatggcttt
     1801 catatggcta taacagattg gaatgactcg gtttgtaatg aaatggagga tatggtgaaa
     1861 gagggagtat cttcatttaa actgtatatg gcgtataaag gaagccttca agttgatgat
     1921 ggtgtaatct ttgaagcatt gagaaaagca gaagagatag gagggattat tggtttccat
     1981 tgtgaaaatg gggacataat atgtgaactt gtggataaag caaagtcaga aaataattta
     2041 tctccaaaat atcatcaatt aactagacca gctataatgg aggcagaagc tacatcaaga
     2101 cttatgaaga tagctgaagt tgcaaactct aaagtatatg tagttcactt aagctgtaaa
     2161 gaatccttag aggaggtttt aaaagctaag caaagaggtg tggatgtaat agttgaaact
     2221 tgtcctcaat accttctatt aaatgatgaa ttatatgatt tagatggatt tgaaagtgct
     2281 aagtatgtaa tgtcaccacc tttaagaaat aaagaaaata atgaaatatt atggaatgct
     2341 attttagata atcaaataga tactatagga acagaccatt gttcttttaa ttataaggga
     2401 cagaaggagc ttgggataaa cgatttcagc aagataccaa atggttctcc aggtgtagaa
     2461 cataggatga gtcttttata cacatatgga gtctgtaaaa ataaaatatc tatcaacaag
     2521 atggtagaag taacatcaac taatgcagca aagttgtttg gaatgtatcc taataaagga
     2581 actatagaaa taggcagtga tgcagatata ttagtcttaa atccaaacaa aaaatccaaa
     2641 atagaagcga aaaaacaatt acaaaatgta gattatacac cttatgaagg gtatgaagtg
     2701 gattgtcaaa tagaacatgt ttatttaaga ggaaaagaag tagttagaga tggagaatta
     2761 atctgtaata ctccaagtgg gagatatata aataggcgtc taacaaaggg ggctagagaa
     2821 tggtagaatt tatattaaat ggagagaaag ttgttgcaaa agaaggcaaa aaacttttag
     2881 acttccttag agaagatatg aatataactt cagttaaaaa tggatgttca gaaggtgctt
     2941 gtggaacatg tatggttata atagatggta aagctactaa agcatgtgta tttaaaactg
     3001 ataaattagc tgggaaaaac ataacaacta tagagggatt atcagagaga gaaagagatg
     3061 tttttgcata ttcttttgct agtcaaggag ctgtacaatg tggattttgt attccaggta
     3121 tggtaattag ttcaaaagca cttatagacg ttaataataa tcctactgaa gatgatataa
     3181 agaaagcttt aaaaggtaat atatgtagat gtacagggta tgtaaaaata attaaagctg
     3241 tcgatttagc agcaaagatt tttagagaaa atatagatgt gccaaaagta aactgtaaag
     3301 gtctagtagg tgaaaatctt cctagagttg atgcagttgg caaagcactt ggatattctg
     3361 aatatgtaga tgatatgaga attgagggta tgatttatgg ctcagcagtt agagctaagt
     3421 atcctagagc tttagttaaa aaaatagata tagaagaagc aaaagcttat ccaggagttg
     3481 ttgcagttct aacagctaaa gatattccag gaactataaa ggtaggacac ttaaagaagg
     3541 attgggatac tttaatacca gaaggtgaaa taactagata cttaggagat ggaatagttt
     3601 tagtagcagc agaaactaga gaagcgttag aagaggccaa aaagctagtt aagatagatt
     3661 atgaggaatt aacaccatta agtactacag cagatgcact tagagaagat gctccaaaga
     3721 tacatgagac agggaatata cttgttaagg aacatttagt aagaggaaac gctaaagaaa
     3781 agatagaaaa ttcaaaatat gttgtaacta aaagatatac tacacctgct acagaacacg
     3841 catttttaga gcctgaatgt gcagtagctg gtccttatga agatgatgga gtaataatct
     3901 attcaacaga ccaaggggtg tttgccactc aacatgaatg tgcagatatg ttagggttac
     3961 catatgagaa ggtaagagtt ataaacaaaa tggtaggagg agggtttggt ggaaaagaag
     4021 acatgagtgt tcaacaccat gcagccttac ttgcatacca tacaaagaga tttgtaaaag
     4081 tacagttaac aagaaaagaa agtattattg ttcatccaaa acgacatgcc acagaaatgg
     4141 aatttacgac agcatgtgat gaaaatggat acttaacggg tatgaaggca acgataatat
     4201 ctgacacagg tgcatatgcc tctcttggag gaccagtgct acaaagactg tgtactcatg
     4261 cagcaggacc atacaattat caagatattg atataacagg aacagcagta tatactaata
     4321 accctcctgg aggagcattt agaggatttg gagttacaca gagttgtttt gcaactgaat
     4381 gtaatctaaa tttacttgct gaaatggttg gaatatctcc ttgggagata agatataaaa
     4441 atgcaataag acctgggcaa gttttaccaa atggtcaatt tgcagatgaa ggaacagcaa
     4501 tggcagagac actagaagct gtaaaagaag agtatgaaaa gcatccacgt gcaggtatag
     4561 cttgtgctat gaaaaatagt ggggttggag ttggtatacc tgatgttgga agatgtagat
     4621 tggtgataaa agatggcaaa gttcacataa gaacaagtgc ttcttgtata ggtcaagggc
     4681 ttggtactgt tatgactcaa gtagtttgtg aaactgctag cctacttcct gaaaatatag
     4741 tgcatgaagt tccagatact catttaacac cagactctgg taatactacg gcttcaagac
     4801 agacagtatt tacgggagaa gctactaagg ttgcatcaaa taagttaaaa gaagctttag
     4861 actctggcaa aactttactt gatttagagg gagaagagtt ttatggagaa tatgaaagta
     4921 ttacagataa aatgggttgt gacaaagaaa atccagtaag tcatgttgct tatggatatg
     4981 caacacaagt tgtaattctg gatgaaaatg gaaaactaga aaaagtagta gcagcacatg
     5041 atgttggaag agctataaat ccaatcagtt tagaaggtca aatagaaggt ggagtagtta
     5101 tgggtcttgg atatgctcta accgaagatt acccagtagt caactctgtt cctacagcta
     5161 agtttggtac attaggttta tttaaatcta cagatatacc agaaatacaa gctttaatag
     5221 ttgaaaagaa caaagattta ttggcttatg gggcaaaagg tgtaggagaa atttgtacag
     5281 ttcctactgc accagcagta caaaatgcct attatgtatt tgataaagaa tttagaactg
     5341 agctaccact acaaaatact ccatatagta aaaaaaagta acaaaaaaaa gaaatattaa
     5401 ataaaaaaat agatgaaatt gtaaataaaa aaaacccttt tgcataaggt atattttaaa
     5461 tgatagaata tacatataca ggagggtttt tcttttataa aataagttta ataaaattga
     5521 gaggtgagca gaatgaattt taataatagt ctagaattaa ctcagtctca gaagctaata
     5581 atgactacac aacttaagca atcattatca atattaaata tgagtaagtt agagttagaa
     5641 gaagagataa aaagagaagc agagaataac ccattagttg aagtcgagaa aagtggggag
     5701 ataaattggg aagagtatat aaaagacatg gataggtcaa gacgtttaga tagaacagag
     5761 ataagctata atcctgataa tgaagttaat ctagaaaatt tagttaaata ttcgtcaaat
     5821 ttatatgaag atttaaaatt tcagataagc ctgtataaac ttacagacaa agaattggaa
     5881 gtctgtgagt atataattga tagtttagat gaggatggat atttaagaac tgaggaaaaa
     5941 gtaattgttg ataccttaaa gatagatgaa gaactttttg aaaaatgttt aattagtgtt
     6001 cagcagttgg aacctagtgg agtaggggca agaagtcttt cagaatgtct gatgatacaa
     6061 atggctagtt taggaatata taatgaaatt ttagaggaaa tcgttacaaa agacttaaat
     6121 ttaattgcaa ataataaata caaagagata agtaaaaagt acaatatgtc attacaaaaa
     6181 tgtgttgaac taataaacat aattaagaca ttagacccca aaccaggaag aacttgttca
     6241 gtagagaaaa gtgtatacat tcaacctgat gtaacagtag aaaaaattga tgatgaattt
     6301 attgtatatt taaatgagaa agattcttat caaataagaa tcaatagtta ttataaagac
     6361 atattaaaaa attcacagtc tgatgaaagt gcaaaagaat ttataaaaga aagactaaat
     6421 tcagcaacag gacttatgaa aaatatagag agtaggaaaa ctacagtact taagattgca
     6481 gaagagatag taaaagctca agatgaattt ttgagaaaag ggacaaaata tataaaacct
     6541 ttgaaaatga aagacatagc agaaaaatta gaatttcatg aatctactat aagtagaggt
     6601 gtaaatggta aatatatgct aacacctttt ggcgtatatg aatttaggta ctttttcagt
     6661 agcgccattg agactgagaa taatgaaatg gcatctagta caagcataaa aaaaattata
     6721 aaggaaacaa taaaagacga aaataaaaag aaacctttaa gtgatgacca tatatctaag
     6781 ttattaaaag aaaaaggaat caatgttgca agaaggacag ttgccaaata tcgtgaagaa
     6841 ttaggaatcc catcttcaag taagagaaaa gaatactaat aagtaaaaga caaatatcag
     6901 tatggtaagg ggaaaaggct aattaagaga tgaatgtaga ttgggctaaa aattttataa
     6961 agtaaaataa tagaatatgg aaaagtatta aaatgctata aggtattaaa aaactgggga
     7021 acattaaaac attaagatag caaagaacat aaaaaactaa agaagattaa aacattaaag
     7081 aagattagag ctttgaggaa tattagaact ttaaagaata ttaaaattta taaaatataa
     7141 aaatactaaa aagaattgaa aaactgggga acattaaaat attgtaaaat gctaaaaaac
     7201 tagaaaacat aattaaataa gtgcttataa a
//
LOCUS       DACXMZ010000199         7209 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.200, whole genome shotgun sequence.
ACCESSION   DACXMZ010000199 DACXMZ010000000
VERSION     DACXMZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.200"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            408..980
                     /locus_tag="JD016_15765"
     CDS             408..980
                     /locus_tag="JD016_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HAU5001079.1"
                     /translation="MKKLVKILINIICIITLTFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNSNYEFKDYLNYIT"
     gene            complement(1991..2653)
                     /locus_tag="JD016_15770"
     CDS             complement(1991..2653)
                     /locus_tag="JD016_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001080.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(2670..5225)
                     /locus_tag="JD016_15775"
     CDS             complement(2670..5225)
                     /locus_tag="JD016_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAU5001081.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLLRLMGAKKRNVRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGIISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLIAVVVSNRDFESMNIIEGIKENE"
     gene            complement(5459..5653)
                     /locus_tag="JD016_15780"
     CDS             complement(5459..5653)
                     /locus_tag="JD016_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HAU5001082.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSSDDIERITKEKYTKEN"
     gene            complement(5665..5844)
                     /locus_tag="JD016_15785"
     CDS             complement(5665..5844)
                     /locus_tag="JD016_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001083.1"
                     /translation="MIHVEISEKEFFRKIEEVFDKGEGNVEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(5849..6106)
                     /locus_tag="JD016_15790"
     CDS             complement(5849..6106)
                     /locus_tag="JD016_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001084.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQSSQVLH
                     YSMTKDKKGTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(6367..7135)
                     /locus_tag="JD016_15795"
                     /pseudo
     CDS             complement(6367..7135)
                     /locus_tag="JD016_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
BASE COUNT         2432 a         1101 c          729 g         2947 t
ORIGIN      
        1 ctcctgcatt tcctgcatta gtactttctt ctatagtatc tgcgaaagta tttgacgcat
       61 taaatgatgc tactgatcca agtcccattg agatacttaa aatcattgac agtgctttct
      121 ttttacattt ttgttgcata aattccccct ccccttaata atttttaata tacactgtat
      181 acatccttat atacactcta ataattctta atatacactg tatacatcct tatatacact
      241 ctaataattt ttaatatata cttaaaactt atatttgtca atgaatatac agtttttttc
      301 aaccttatat attataagta ttcattattt gctaaataac tacaataaaa attttaatta
      361 tataaaattt atttaatctt ttatataata atacaagggg taattaattg aaaaaactag
      421 taaaaatatt aataaatatt atttgcatta ttacgctaac tttctctagc ctttcgattt
      481 acataaaatt atctgaatat aaaaaagctg atgaagtata tacggaactc agagaaaaca
      541 ctatcaataa tagcaaacat caagaacttt atgataaaaa taatgattat agattctggc
      601 ttaaaataaa caacgtaaat atagactatc ctgtagtaca aggatataac aatgatttct
      661 acctaactca tgatttttat aaaaattatc ttcctttagg tagtattttc atggattata
      721 gaaataattt tgaaaatgat aagagtttaa tagtctatgg tcattatatg aaaaataaaa
      781 ctatgtttgg tcagctagaa aactatactg atgaagtatt tttcaaagaa aataacttag
      841 tagaaataaa ttataaagta caaacttata cttatgaaat tttttcagta tatactgctg
      901 acttaattaa cagagattat ttaagtatac actttaatag taattatgag tttaaagatt
      961 atttgaatta tataacctaa aaatcagtac acaaatctga tataaaggta gatatttcag
     1021 ataaaattat aactttgtat acttgtaact atgaatttaa agatgctctt actatagttc
     1081 atgctaaatt aatttcaaaa aaagagactg ataagtaggt ttgtgtaaat gcagacttac
     1141 ctacagtctt ttttgaacgt aaaattggta agtatggaat aatatttata ttaaactttt
     1201 gtaggcaaag aaatgaaaaa tcaaagagaa ataattatta cagatttaga ttaccaagct
     1261 gaattaagaa agtgtaaaac tatggaggat gtagttggtg aagatagtat ataatagttt
     1321 aacttataaa ttatcttggt gatattaatg aagtttcaaa gtatatatta tttattgata
     1381 taaataaaat taaatctgtt gctaatagat gtacctttgt ttggagacaa actattgaag
     1441 aaatataact aaaatgttga agtagctacc tgactttatt ttgaaatgcg aacaagattt
     1501 tggcattaag ataatttaga aagaatcact ggtacacaag gatgtgaact tagaatgaat
     1561 agaagtattc aggcataagg agctttcacc caaataaaac atagtatgaa ctttaaaaga
     1621 attctaatca gagacaatca aaatgttctt tcatagttta tacctgtagt gttagacata
     1681 gtatttctaa gtttcacaaa taagataatc attaataaat gtagtatgta tattcatcct
     1741 ctcaaaaata ttttttaatc ttacactttg taaattattc tattttaaaa tggtattttt
     1801 atacctttat ttcttatgac cgttttcaag ctcaaatttt gcataaatat aaattaatga
     1861 aaagcaagta aaaaaagaac tgtcgcacag caatttaaaa ttactcattc gacagtcctt
     1921 ttttcgtgga ttaatggtgg agaagaatca caagaataat acccacgatt aaacctttat
     1981 acaatacatt ttatatgact ttaccatcac taatagttat tatacaatca gccatcttag
     2041 ctatttcatc attatgagtt atcataacca acgtctgctt gtattcattt acacaatact
     2101 ttagtaaatt catcacctca ttagttgttt tactgtctaa atttccagta ggttcatctg
     2161 caaaaattat tgatggctta ttggcaagtg atcttgctat tgcaactctt tgttgttgac
     2221 cacctgataa ttcattagga aatttattaa tctgactttc taatcctaac ttagttatta
     2281 aatcattaat ataattatta tctatctttc ctttagcaat taaaatagga agaactatat
     2341 tatcataaac attgataaca ggtattagat tatagttttg gaatataaac ccaaattctt
     2401 tacttctaag attagatagc ttttcatcac ttaaagtata taaatctaaa ttatccataa
     2461 atacattacc taaagttggt ttatcaagcc ctgccataca gtgaagtaat gtgctttttc
     2521 cagaaccact ttgtccaatt attgcactaa atttatgggg ttctatctct aaagtaattc
     2581 catcaacagc ctttatttca ttttctcctt ttccatatat ttttttaata tctataactt
     2641 ttaacatctt catacattta cctcccaaat tattcattct cttttatacc ttcaataata
     2701 ttcatacttt caaaatctct attacttaca actactgcta ttaaacatac aaatacagat
     2761 atagcaaaga atagtaaaga ttccttaatt gggaatgtaa aagacataaa attctctatt
     2821 ccatagttta tatacgcatc attattccaa tttgtaaatt taactagatt tataaatgat
     2881 ggaatcatag ctattataaa gctaaaaact gcatatgcta atgcttcata tataagcatt
     2941 tttttcatat ttttaagact aacacctaat gctctaagcg ttgaaatttc tttttttcta
     3001 attagtagat ttgttcttat agtacagaaa atattaattg atgctattat taaaactagt
     3061 gcaattattg ctatttgtga tttaatgtaa cttttatata tactttctga catttcatga
     3121 taacttaatc tactattaaa tagagaccct ggtatacttc ttgaaatttt ctgtatctca
     3181 ttattaacaa tctcatcata tggattttct aaatttatgc ctaattgagt gtatttttgt
     3241 tctcctgtta tgtttttaga gtgaatatta cttgttataa tttctagatc tggcataaaa
     3301 tcgccatctc ccatagcaat ccattcatcc tttaaaatta cagccactct taccttcatc
     3361 tctttataaa caattgtatt attttcttca acaggaattt ttacagttat tatgtctcct
     3421 atatttaatt ctttgaatat aggctttata gtatgatcat caactatatc ataaaaataa
     3481 ttatatactg ctacatttgg atatccatca gtgtcctttt taatatcatc taagctacct
     3541 tcttcaacaa agtcatttaa cttttcatct atataatcat catcataaag ctttaaaact
     3601 aatttatctt ccattatctc ttttttttct actccattat aggttgtaaa ttctttgttt
     3661 atatccttat tattaaaagt aaacattcca tttgttattt gaattttatg tatactttta
     3721 acacccttaa ttttttctat attctctaca tcactatccg tgtattttga tatttgcggg
     3781 tctgcattta ctccaaattg cattgttata ttatatctat tttgcatatc ttcaatttta
     3841 ctgttgacct cttcttgcat acttgagaat gtagtaatga atatatatcc tcctaaagca
     3901 atagacaaca tagatatagc agctctaacc ttatttcttc cgatattttt aagtcccata
     3961 aatccataaa atccaaatat tttgcttact agtctgtcta ttccaagtct acttctgtga
     4021 tttttaattt tgtctgtatt tattattgat tcaataggtg atatcttgcc gattttaaaa
     4081 gttggtataa tacaagatat aaaaacagaa attattccaa ccaatatagc ttttataact
     4141 atttcattat ttatatataa tttagggtta agaccagctt cactgtataa aacttcgtta
     4201 taattactaa ttcctatata tgaatatgta accccagcta ctaacccaag tagtatacct
     4261 attattgtta taattaatgc ttgatagatt atcatacacc ttacgtttct tttctttgcg
     4321 cccatcagtc taagcagtcc catttctttt actgattgat ttagtgtaat attgaaaata
     4381 ttaaatatga aaattataga tactgtagtt attaaaacat ttcttttaaa aacattcgga
     4441 tcattcttat catcttctac ataacgaagt tcccttacaa gaggtacatt tggtagaaag
     4501 cttttttgtc ctaacttata ttttttcata agataatcac attcaccttc aatatttctc
     4561 catccacttt taaaacttaa tatactatta taactaatcg catcatgagg tataatgtta
     4621 tttgtttcat cattgccata agttactgct tcgtattgcc aaaccacatc atagtaccct
     4681 tttggttttt ccataactcc tactatttta aattctttat ttatagaaaa taattgatta
     4741 cttccgttag tatctttata gttctttttt attgttaaat ttaatgtaga tcctactcct
     4801 tcacttaaat tcattgcttc tagtgctttt ttctctaata ctatttcatt gttgttttta
     4861 ggtagtctac cttctattat tttgttgtat ctacttttta gatactcttc gtttgatgaa
     4921 gttaatataa atgagtttcc ttttttatct gaaatacgtc ctagattttg taccgaatta
     4981 ccctttgata ctttttcatc agtttctaat tttttagcat catcaagtgt caaatcacta
     5041 aactctacat ggtatccact gtaaagttca tgtattgcta ctacattgtt ttcattttgt
     5101 gaatctttta taacactagg tccaaatatc aacataacac ctaacataac tcctattatt
     5161 aaaagtaatg ttctaagttt atttttcttt aaatatgcta gtgacatttt taagtaaatc
     5221 cccatataat cctcctattt tactataaat tatttatcca actcatctgt taagtagatt
     5281 ggtggatatt ttaaatcttt taattcttca ttattttttg aagcttacta ccatcttcct
     5341 catgataaac ttgcagtatc accttattta catgtagtgc ataataatca ccattataaa
     5401 aatattagta tttataacct ggatagtttt tgtaaaatga tttataaagt atcccatcct
     5461 aattctcttt tgtatacttt tcttttgtaa tacgctctat atcatcactt gaaactcctt
     5521 gatatgtcat atatttatct atatattttt cagcttcatt ccattttcta aaatagttgt
     5581 tgtatataat aaatcctatt atggctataa tgcttataat taaagttaat aaaatagctt
     5641 tgtattttct catagtgaat cttcctactt caatttaaat tctttaataa gttttaattc
     5701 taaagactta gttttatcat caaatttctt aacttgaatt tttatatctc ccaaattatc
     5761 aacctctaca tttccttcac ctttatcaaa tacttcttca atttttctaa aaaattcttt
     5821 ctcacttatt tctacatgaa tcaatgatct atatgttata aatagccatt atgaatttat
     5881 catcttctga cagtttttca ttataagaaa aatctttaac acattgaatt gataatattt
     5941 cctttgtacc cttcttgtcc ttagtcattg aataatgtaa aacttggcta gattgttctt
     6001 ttgtatactc caagtttccc aaagacaaaa caaaacttcc atctgattta ttttcagcta
     6061 ccctaagacc actattttct attgattctg atattttctt tgacaagtct tcttgatact
     6121 agaaatctgt tcaatagtat atattttttc atttgtatct gaaatcttat ttgtgcatcc
     6181 tgttattaaa cctagtgtta agcttatagc tataaccata gttattgtaa tttttttatc
     6241 ttcatataat cctccttata tattgaaaac tcgttatttc tcataaaaaa aaagacgcta
     6301 tctttttcta ttaaaagata acatccctat atgtttagta tcaatatatg ttccccaact
     6361 ggtaggttaa tcgacccaac tatcagtatt ttgatttaat ggtatataaa tattcaatat
     6421 aaatttgtct tttttatctt caaataataa atctcctcta tattttttta tagaagattt
     6481 gatgctttga atccctattc catgtacaag tttatctttt ttatctgtta ataataaact
     6541 tttattcttt ctaattgaat ttattttact attctcacat ttaattacga aataggactt
     6601 tgagatagtt ccttttattt gtatatattt atcaatactt ttatcatgta tcttgtcgca
     6661 agcttctatt gcattatcta agtatttgca aatatattag atacatctat tggttttata
     6721 aaattcactt tggaaaaatt gatatcacaa atgaatttaa tttttctttt ttcacttaca
     6781 tcactttttt catttattat tatatccaat ataacattac ctgtattgta aattgattta
     6841 aattctttaa tttaatcttt taaactattt acatacccat caattttcct tttttatatt
     6901 tgagattttc tatacaatat atatgattat ttatatcatg ataaagcttc ttaactttag
     6961 cgtgtgattc ttgaatactt agataatgag aatactgcat atctaactta ttttttatta
     7021 cttcattttg caattttaac ttattttcct taattatttt tataaacact ataataatga
     7081 ctaagctact agctagtatt aacctaggta tcacaatata cccaactata tatatttcct
     7141 aatacccacc aaatagatac atttttctat ttatataaat tattaatata gtaaataaat
     7201 ttgttatta
//
LOCUS       DACXMZ010000200         7173 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.201, whole genome shotgun sequence.
ACCESSION   DACXMZ010000200 DACXMZ010000000
VERSION     DACXMZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.201"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            53..1453
                     /locus_tag="JD016_15800"
     CDS             53..1453
                     /locus_tag="JD016_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAU5001085.1"
                     /translation="MELDVIKKLIEQTNSKHNNFVKKADEAEKYYKNQNDIIRDRSPN
                     NVGKVNTANNPLRNADNRIPFNWFGFLVNQKVSYLFTYPPTFDVGDDGINSKITDILG
                     DRYPKEAKTLGKNASICSKAWLHVWEDDNNDFQYANIDPRQIRAVYSSDLNRKLLAVL
                     REYKKTDDEGKEYVIYEYWTDECCYTYQNKDGNSNINGLEILNKFIEKNLDNKLETQT
                     NVYKHNFGEVPFIEFLNNDLEVRDLDNVKHLIDVYDKVYSGFVNDIEDIQEVIFVLTN
                     YGGADLTEFLKGLKEYKTIDLQSSGADDKSGLSTITINIPIEARDSLLKTTEKQIYVQ
                     GQGVDPKPENFANTSGVALKFLYTLLELKAGLMETEFRLGFAKLVRIICKHIGYSPKR
                     VLQTWTRNMIQNDLELADICSKSVGIVSEKTNLKNHPLVDNAEEEEKQIKKEKEYNQQ
                     EYNSIIPNNSGVINET"
     gene            1443..2471
                     /locus_tag="JD016_15805"
                     /pseudo
     CDS             1443..2471
                     /locus_tag="JD016_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
     gene            2785..3045
                     /locus_tag="JD016_15810"
                     /pseudo
     CDS             2785..3045
                     /locus_tag="JD016_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197899.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3292..3564
                     /locus_tag="JD016_15815"
     CDS             3292..3564
                     /locus_tag="JD016_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001086.1"
                     /translation="MFKKKYIKKPSKISVRNIIAFIITVIGIALGVFIGINIIMAHVL
                     GIANMVDNNTFTCVRLVYNLVGVISGYLIGKAIYLIASLISYIIYE"
     gene            3615..3845
                     /locus_tag="JD016_15820"
     CDS             3615..3845
                     /locus_tag="JD016_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001087.1"
                     /translation="MKEYVIWFKSGNCVSGITDEYVADKLMKDFIEADSDCRNLKGYL
                     DEDGTTIIDLSQIEAISINNCSENNNIGFSKS"
     gene            3871..4464
                     /locus_tag="JD016_15825"
     CDS             3871..4464
                     /locus_tag="JD016_15825"
                     /inference="COORDINATES: protein motif:HMM:PF06810.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001088.1"
                     /translation="MKGELNKMKKGELIALGLSEEDAKKVEAESLKELENYINKIEYE
                     KVKEELKASKEAIEGFKDGMTKEQIEELKKGYETKLTAKDEEYQKKLKEKEQKEFDMA
                     LENELIKLNVHSTKAAKAELDLEKIKYENGAFTGLKEQTDTWSTQKSFLIKTGETKIN
                     YNPDNGKKNTLSRAENIAKEKNEEGSKNPYADAWSIK"
     gene            4480..4860
                     /locus_tag="JD016_15830"
     CDS             4480..4860
                     /locus_tag="JD016_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001089.1"
                     /translation="MYFKEVNFDNTPEFLASQHYINFSKTALDTDVVADENGKKYVLA
                     GSLLGESGKVVKITRGGSSGSYTYTLSEDPVGIVFSTVDVTYGPQPVASMVEGYVITE
                     RLQGEYVKEAIDTIKTKLPNIKFM"
     gene            4872..5891
                     /locus_tag="JD016_15835"
     CDS             4872..5891
                     /locus_tag="JD016_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HAU5001090.1"
                     /translation="MARVEELLSVQELINYTKTRKLKETMGDLLFPTQKIEGLEIKMI
                     KGASNLPVSASVHAFDTEAEIASREGANLSIAELALVKRKIKLDEKDIIVLEEPRNSQ
                     EETQMINQIFNDVDNLVSSVNTRIEAMRMEVLTTGELNINENGVKASLKYGTPTNHKE
                     TKTWSSGTPDILGDIYNMTDKIVVDTGFTPTRSLTSKTILNTILRDEKLRKAIFGVNS
                     DKLLTLKELNVFLASQSLPPIFTYDERYRVQGKDGKYTTKRFLDENKFILMPDGKMGD
                     TFFGLTAEELELRKNPSIDISSVGNIIVEQYSTADPVAKWIKAVATVLPSFPYADQVF
                     MGTIN"
     gene            5902..6246
                     /locus_tag="JD016_15840"
     CDS             5902..6246
                     /locus_tag="JD016_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001091.1"
                     /translation="MEVERLKKLLGFSREDDSKDTILEFILEDVEEMVKNYCNVPTIP
                     EQLNSTILRMAIDMYKNESLGSEDIALGSISSISEGDTSVSYRSSASEFKESLLKDYK
                     SQLNRYRKLRWK"
     gene            6246..6608
                     /locus_tag="JD016_15845"
     CDS             6246..6608
                     /locus_tag="JD016_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001092.1"
                     /translation="MMDKTRKAIEMLYRDKCTIVEYQPIKDPVTKRANNKEVVVLENQ
                     PCKLSYKNITSATDGKVAKLVQTIKLFISPNINIKAGSKLIITTQNNITKEYIRSGES
                     AIYPNHQEIILELFKDKA"
     gene            6608..7033
                     /locus_tag="JD016_15850"
     CDS             6608..7033
                     /locus_tag="JD016_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAU5001093.1"
                     /translation="MARWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTTYASYVEYGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
BASE COUNT         2973 a          752 c         1334 g         2114 t
ORIGIN      
        1 cttaaaaata atggggttag aatacttact ccaaatggaa gaaggtgaga aattggaact
       61 agatgtaata aaaaagttaa ttgaacaaac taatagtaaa cataataact ttgttaaaaa
      121 ggctgatgaa gctgaaaaat actataaaaa tcaaaatgac attataagag atagaagccc
      181 taataatgtt ggcaaagtaa atacagccaa taatccacta agaaatgcag acaatagaat
      241 accatttaat tggtttggtt ttttagttaa ccaaaaagta tcatatctgt ttacttatcc
      301 gcctactttt gatgttggag atgatggtat aaattcaaag ataactgata ttttaggtga
      361 tagataccca aaagaagcca aaacacttgg gaaaaatgct agtatatgct ctaaagcatg
      421 gttacatgtt tgggaggatg ataacaatga cttccaatat gctaatatag accctcgcca
      481 aataagagca gtatattcat cagatttaaa cagaaagctt cttgcagtac ttagagaata
      541 taaaaagact gatgatgaag gaaaagaata tgtaatctat gagtattgga cagatgaatg
      601 ttgttatact tatcaaaata aagatggaaa tagtaatatc aatggattgg agatacttaa
      661 taaatttata gagaaaaatt tagataataa acttgaaact caaactaatg tatataaaca
      721 caattttgga gaagttccat ttatcgaatt tctaaacaat gatttagagg taagagattt
      781 agataatgtt aaacacctca ttgatgtata cgacaaggtt tatagcggtt ttgtgaatga
      841 tattgaagat atacaagaag ttattttcgt tcttacgaac tatggaggtg cagacttaac
      901 agagttttta aaaggactta aagaatataa aactattgat ttacaaagta gtggtgcaga
      961 tgataagagt gggcttagta caattacaat aaatattcca atagaagctc gagattcgct
     1021 tcttaaaaca acagaaaagc aaatatatgt ccaaggacaa ggtgttgacc ctaaaccaga
     1081 gaatttcgct aatacaagtg gtgtagcact caaattttta tacactttat tagaattaaa
     1141 agcaggtttg atggaaacag agtttagact aggatttgct aaactagtaa gaataatatg
     1201 taagcatata ggatattcgc ctaaaagagt tttacaaact tggactagga atatgattca
     1261 aaatgattta gaactggctg atatatgctc taagagtgta gggatagtat cagaaaagac
     1321 taatttaaaa aatcatcctc ttgtagataa tgcagaagaa gaagaaaaac aaattaaaaa
     1381 agaaaaggaa tacaatcaac aagaatacaa tagtatcatt cctaataata gtggtgttat
     1441 aaatgaaaca taaagactat tggaggaaaa gatttgaaca attagaagaa gctcaaaata
     1501 acaaaagtat aaaatgctat cttgaattag aaaagcaata taaattagct atgactagta
     1561 tagaaaaaga tatattagca tggtacaaca gatttgctga aagtgaagga atatctttat
     1621 tagaagctaa gaaactacta aatacaagag aactagaaga gtttaagtgg aatgtagaag
     1681 aatatattaa atatggtaaa gaaaatgcta taaatcaaaa gtggataaaa gaattagaaa
     1741 atgctagtgc tagagttcat attacaaggc ttgaagcttt aaagttacaa atacagcaac
     1801 aagtagaagt tttatatgga aatgaaattg atggtattga taaactaatg agagatattt
     1861 atacaagcga gtactatcat acagctttta atgttcaaca aggagtaaac gttggttgga
     1921 gtttaatgag tcttgatact aatagaataa ataaaattat ttctaagcca tggactagtg
     1981 atggattaaa ttttagtgaa aggatttgga gcaaacatag acctgcttta attaatgagt
     2041 tacacactaa attgacgcaa tcaattatta gaggtgagaa tccaaaaaat ctagtcaatg
     2101 actttgctaa gaagtttaat gtatctaaat cacaagctaa gaatttaata atgactgaat
     2161 cagctttctt tgcatcagca agtagaaaag attgttttaa tgatttagat gtagagaaat
     2221 atgagattat tgctacatta gatttaagaa cttcaaatat atgcagagaa ttagatggta
     2281 aagtatttga tatgaaagat tatcaagttg gaataacagc tccaccattt cattgtcgtt
     2341 gtaggacaac gacagctcct ttttttagtg atgaagaagg ctatagagta gcaagagatg
     2401 aaaatgggaa aacatattat gtaccatcta atatgaaata taaagagtgg cataagaagt
     2461 atgttaaaaa cacttactaa gttagtaggt gtctttatta tgtaaaaaaa ttattgagag
     2521 ggtgatttga aatgcttaaa ttatatattt tatcaataat tgtgttttgt acagggcttt
     2581 atttattcaa catgaaggtt tatagtaatg aggaattaat tgaattactt aaaagtgtga
     2641 atatcagaaa aagaaaaaaa tataatttta tttttctagc tttatttcca ctacttaatt
     2701 ttattcttgg tgtgatactc atactatatt ctttgctagt tagcaatgaa gatattatta
     2761 aatatttaag gaggaataaa taagatggct aagtttaaaa agaaagcagt tgaagtagaa
     2821 gcttttaaac taggttatga tgtagaacca aaatggttta ttgagaatga tagagtttgt
     2881 aattttatgc aagaaaaatg tattaatggt catataagtt gtgatttaaa gacactagaa
     2941 ggtactatga gagctaataa aggggattac attatacaag gtgtaaaagg agaaatatat
     3001 ccatgtaaag cagatatatt tgaaatgact tatgagaaag ttgaatatag agaaaaaaat
     3061 aaattatcaa cagagatgac tttagattca acaaactttc aagaaaatat taaaaaagct
     3121 agaaaagaat tagatttatt tatacaaact ttagaaaaag cagatgataa aattaataaa
     3181 ctagcagaaa aaatgaataa atgtgattgt aaagttgata tagataagat tgtaaaacag
     3241 ttagtggaac acctaagaga aggtattgaa taagttttgg gggatggaaa tatgtttaaa
     3301 aagaaatata ttaaaaagcc aagcaaaata agtgttagaa atattatagc atttattatt
     3361 acagttattg gtatagcttt aggtgttttt ataggtatca acataattat ggctcatgtt
     3421 ttaggtatag ctaacatggt agataataat actttcacat gtgttagatt agtttataac
     3481 ttagtagggg ttataagtgg gtatttgata ggaaaagcaa tataccttat agcttcacta
     3541 ataagctata ttatttatga ataatttgtt taaaggtttt ttattaggta aaaaacaaaa
     3601 ggagggttgt attattgaaa gagtatgtaa tttggtttaa gagtggaaat tgtgtatctg
     3661 gaataacaga cgaatatgtt gctgataagc taatgaaaga ttttattgaa gctgactcag
     3721 attgtaggaa cttgaaagga tatttagatg aagatggaac aacaataata gatttatcac
     3781 aaatagaagc tatatcaata aataattgta gtgagaataa taatattggt tttagtaagt
     3841 cctagatagg gcttttttat tatgtaaaaa atgaaaggag aattaaataa aatgaaaaaa
     3901 ggtgaattaa ttgcattagg gcttagtgaa gaagatgcaa aaaaagtaga agctgaatca
     3961 ttaaaagaat tagaaaatta tattaataaa attgagtatg aaaaggtaaa agaagaatta
     4021 aaagcatcaa aagaagcaat tgaaggtttt aaggatggaa tgacaaaaga gcagattgaa
     4081 gagcttaaaa aaggctatga gactaaatta actgcaaaag atgaagaata tcaaaagaaa
     4141 ttaaaggaaa aagaacaaaa agagtttgat atggcattag aaaatgaact tattaaactt
     4201 aatgttcata gcactaaagc agcaaaagca gaacttgatt tagaaaaaat aaaatatgag
     4261 aatggtgctt ttacaggact aaaggaacag actgatactt ggtcaactca aaaatctttt
     4321 ttaataaaaa caggagaaac taagataaat tacaaccctg ataatggcaa aaaaaataca
     4381 ttaagtagag ctgaaaatat tgctaaagaa aagaatgagg aaggttctaa aaatccatat
     4441 gctgacgcat ggagtataaa ataaaaagga ggataaagta tgtattttaa agaggtaaat
     4501 tttgataaca cacccgagtt tttagcttct caacactata ttaacttttc aaaaacagca
     4561 ttagatacag atgtagtggc tgatgaaaat ggaaagaaat atgtgttagc aggtagttta
     4621 ttaggtgaga gtggcaaagt agtaaagata actagaggag gttcttcggg tagttataca
     4681 tacacattat cagaagaccc tgtaggaata gttttttcaa ctgtagatgt tacttatgga
     4741 ccacaaccag ttgcatcaat ggtggaaggg tatgtaataa ctgaaagatt gcaaggtgag
     4801 tatgtaaaag aagctataga cactataaag acgaaattac caaatattaa atttatgtag
     4861 gaggatgaaa tatggcaaga gtagaagaat tattgtcagt tcaagagctg ataaactata
     4921 caaagactag aaaattaaaa gaaacaatgg gagatttatt atttccaact caaaagatag
     4981 aaggacttga aataaagatg ataaaaggtg catctaatct tccagtatca gcaagtgttc
     5041 atgcttttga tacagaagca gaaattgcat caagagaagg tgctaattta agtattgctg
     5101 aacttgcact tgtgaaaaga aaaataaaac tagatgaaaa agatataatt gtacttgaag
     5161 agccaagaaa ctcacaagaa gaaactcaaa tgataaatca aatatttaat gatgttgata
     5221 atcttgtatc aagtgtaaat actagaatag aagcaatgag aatggaagtt ctaacaacag
     5281 gagaacttaa tataaatgaa aatggagtta aagcttcttt aaaatatgga actccaacaa
     5341 atcataaaga aacaaaaact tggtctagtg gaacaccaga tatattagga gatatttata
     5401 atatgactga taaaatagtt gttgatactg gatttactcc aacaaggtca ttaacctcta
     5461 aaactatttt aaacacgata ttaagagatg aaaaactaag aaaagctata tttggtgtaa
     5521 atagtgataa attgcttact ttaaaagaat taaatgtatt tttagcttct caatctcttc
     5581 ctcctatttt tacttatgat gaaagatata gagttcaagg taaggatggt aaatacacaa
     5641 caaagagatt tttagatgaa aataagttta ttcttatgcc tgacggcaag atgggagata
     5701 ctttctttgg attaacagca gaggaattag aacttagaaa aaatccatca atagacatta
     5761 gttcagttgg aaatataatt gtagagcagt attctactgc tgacccagtt gctaagtgga
     5821 taaaagcagt tgcaacagtc ttacctagtt ttccttatgc tgaccaagtg tttatgggta
     5881 caataaatta gaggtgttaa tatggaagtt gaaagactaa aaaagctttt aggatttagt
     5941 agagaagatg attcaaaaga tacaatacta gagtttatac tagaagatgt agaagaaatg
     6001 gtcaaaaact attgtaatgt acctactata ccagaacaat taaatagtac tattttaaga
     6061 atggctatag atatgtataa aaatgagagt ctaggaagcg aagatattgc actaggctct
     6121 atttcttcta tatcagaagg tgatacatca gtttcctaca gaagttcagc tagtgaattt
     6181 aaggaatctt tacttaaaga ttacaagtca caattaaata gatacagaaa acttaggtgg
     6241 aaataatgat ggataagact agaaaagcaa tagaaatgct gtatagagat aaatgcacta
     6301 tagtagagta tcagccaatt aaagaccctg taacaaaacg agctaacaat aaagaagtag
     6361 tcgtattaga aaatcaacct tgtaagttat catataaaaa tattacatca gctacagatg
     6421 gaaaagtagc taaacttgta cagactatta aactctttat atctccaaac ataaacatta
     6481 aagcaggttc aaaacttatt ataactactc aaaataatat tacaaaagaa tatataagaa
     6541 gtggagaatc agctatatat ccaaatcatc aagaaataat acttgagtta tttaaggata
     6601 aagcataatg gctagatggg gcagtgttga ttttagagag tttaaaagag tttgtaaaaa
     6661 gatggagaag cttacaaaga ttgatttaga taagttttgc aaggatgcag caagagaatt
     6721 agcagcacga ttacttggga aagtaattag aagaacacca gttgatacag gattcttacg
     6781 acaaggatgg aatggagtgg cttatgctag gtcgcttcct gtgtataaac aaggaaataa
     6841 ttatattata gaggttgtta atccgaccac atatgcaagt tatgttgaat atggccatag
     6901 aactaaagat ggaaaaggtt gggttaaagg acaacatttc ttaacaattt cagagatgga
     6961 actacaaagc caagttgata agattataga gaaaaaacta ttaatattgc ttaaaggagt
     7021 atttgatgct taataatata attgatggaa tatctattaa attagataaa acatttggag
     7081 agagttatac aatttatagt gaagatgtgg agcaaggtat aaatgaacct tgttttttta
     7141 ttgttccttt aaatccaagc aaagtatcct atc
//
LOCUS       DACXMZ010000201         7100 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.202, whole genome shotgun sequence.
ACCESSION   DACXMZ010000201 DACXMZ010000000
VERSION     DACXMZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.202"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(138..800)
                     /locus_tag="JD016_15855"
                     /pseudo
     CDS             complement(138..800)
                     /locus_tag="JD016_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009109549.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(960..1496)
                     /locus_tag="JD016_15860"
     CDS             complement(960..1496)
                     /locus_tag="JD016_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HAU5001094.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1569..1982)
                     /locus_tag="JD016_15865"
     CDS             complement(1569..1982)
                     /locus_tag="JD016_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HAU5001095.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKLCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCEGIISIHDRECSECQEKVK"
     gene            complement(2522..2713)
                     /locus_tag="JD016_15870"
                     /pseudo
     CDS             complement(2522..2713)
                     /locus_tag="JD016_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2754..3002
                     /locus_tag="JD016_15875"
     CDS             2754..3002
                     /locus_tag="JD016_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001096.1"
                     /translation="MEPDIKDLKTGQIETSSRLDTIQSDVTEIKSDVKINRNTLDTVI
                     KHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3617..3787)
                     /locus_tag="JD016_15880"
     CDS             complement(3617..3787)
                     /locus_tag="JD016_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAU5001097.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIF"
     gene            complement(4013..4546)
                     /locus_tag="JD016_15885"
     CDS             complement(4013..4546)
                     /locus_tag="JD016_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAU5001098.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            4863..5213
                     /locus_tag="JD016_15890"
     CDS             4863..5213
                     /locus_tag="JD016_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001099.1"
                     /translation="MGKVRIGCKKEKYFYVRNPVKSLVDRITENEQFAIYHFTHKYNW
                     RNKVFSILQRLIRLEKSKNELYMYESLVSVSFLWLIMIKNIVLPPIKHKNIINIHMQK
                     ILRYIEKYYCFTLY"
     gene            5182..5500
                     /locus_tag="JD016_15895"
                     /pseudo
     CDS             5182..5500
                     /locus_tag="JD016_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429539.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(5884..6456)
                     /locus_tag="JD016_15900"
     CDS             complement(5884..6456)
                     /locus_tag="JD016_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAU5001100.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(6518..6781)
                     /locus_tag="JD016_15905"
     CDS             complement(6518..6781)
                     /locus_tag="JD016_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HAU5001101.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(6781..7002)
                     /locus_tag="JD016_15910"
     CDS             complement(6781..7002)
                     /locus_tag="JD016_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HAU5001102.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         2502 a         1207 c          868 g         2523 t
ORIGIN      
        1 tagctttagg ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca
       61 tataggtttc cctatatgct gactatttta gctactaact catgactaaa gtcacaagtg
      121 tgcgtagcta ttttttaatc aaactggaat acagactcca ttcctaggaa attggctgaa
      181 atatagtctg ctgtcaaaga tgctgtcatc acagaaatta aaacagatac tgaaaagttt
      241 ttatggactt cttcaagtga aaacataaca cccgccagtg gtgcatgaaa ggctgctgat
      301 aatccagcac ttgccccaca ggttaacagg aatttttcct ctgtcttccc tcggtcaaga
      361 gctctagaaa tacctttacc tgccatagca ccaagctgaa tggaaggacc ttcccttccc
      421 aaagcaagac cacccatgag tgataagaat cctccaataa attttgttgg caacactcgc
      481 caccatactt gttttatttt ccctgtcatt tccccctcaa gctgagggat accacttcca
      541 gaaatcattg gctcccagtc caccaacttt gatacaatcc acgcaagtac tgcaagtact
      601 gcaaaccaag ctattatttt tattgtagaa ccttctacaa attttaaaat ttggctaagc
      661 caagtaccag cctgccctaa aagtatcctg tataaaagca caatcaaacc agccacagcc
      721 ccaaccataa gtccttcacc aatcaaaatt acctgaaagt gctctgcccg tttgataata
      781 tgagatgtat cctttttcat actgttcctc cttcatccaa taattctaca caatctgaat
      841 ctccaatgac taaagtcatg agtgtgtaca gatatttttt aatcaacaaa aatagatttt
      901 tacttactaa atataagaaa ataattgctc aatttctcaa taaatgtgaa tttattcttt
      961 taggtattta ttctccacag tcgtgcttct tccagcaaaa tatttattaa ttcttgcttt
     1021 ctctgtgtat agattttacg attgtcctca aactgtaatg ctaatttttg tttatgacta
     1081 tcatagagtt ttgctttgtc tgtaaaagta tttagataat ctcgaaaatt gatataatta
     1141 ttccatgcaa cactattcca ttcaacaaca tgaatatggt gagtacgagt atttttctca
     1201 aaatctccca tagcaaaaag taattgtttt ggtacatcct gacctcgaaa aacgattcca
     1261 cattgctcta acctttcgag gtataattta gcttcctcta aagaagcaac acctacaaca
     1321 atatcaataa taggctttgc gctaatactg ggaatagaag tgcttccaat gtgttgaata
     1381 tcaatggcag tcttatccca tatacttttc aataatataa tgacatcttt tgcactttta
     1441 tcccatatca tctgatgttc caataattta accataccac attttaaacc tatcactata
     1501 taccacctca atataatcca cctttatata tattacaatt ttttctatct tgataaatag
     1561 tgatttagct atttcacttt ttcttgacac tcgctacatt ccctatcatg aatagatata
     1621 attccttcac atttagaaca agtatatctc tccttctgct tttgcataaa cttctctaaa
     1681 ccgtactgct gaacaaattt actattttcc ataaggcttg cttgatatct cttgttataa
     1741 cttttttcaa gattttttat gagtttacaa ggaaaattga aacattcaaa acaataaaac
     1801 aacccttttt gacagacaca atcctttatt ttacacttac ggcagtgttc tggctttcct
     1861 gtatcgccgt tcaaacaacc agcacatagc tttttgtgat aacaatgctt atagcaaacc
     1921 atacaattca ttccacaagg agcaaacata atcgtatcaa tactctcttt tggcattttc
     1981 ataacatttt actcctttca aaaatccaat ttgtcgaatt gttatatatt catattgtaa
     2041 cataaaaaat agctaaaaaa ttcatgcact ttcattgcca ataaattttc aaggaaatat
     2101 gaaggtttaa aactaagaat aaaaagagat aaaaaggaag ctactaatca aaatattcaa
     2161 acacaagtaa tcaatataaa taacttaagg tatatttaaa agtatatgat attaccagca
     2221 gttttaaata caaatataac cacacatgcc tcaaaaacac ttattacagt atcttagaaa
     2281 cttagacaat acataagatt cattaataaa aaagttctca atatagatat atttaaccat
     2341 gttttgaata gcagataatt atatgattaa aaattaaata tgacatagcg tttgtggaat
     2401 ataacaccta aattatgtta taataataaa acaggagtgt gatagtgatg gatgaaaaaa
     2461 tattagagtt attacaggaa atgcaaggta atattagtaa tatgcaaggt agtatcagtg
     2521 gtatcgaagg cactattagt gatatgcaaa gcaatattaa tggtatcgaa gacactatta
     2581 gtggcatgca aggcactatt agcgatatgc aaggcagtat cagtggtatg aatggcacta
     2641 ttagcgatat gcaaggcagt atcagtagta tggatggcac tattaatagt atgcaagaca
     2701 gtattactga tatgcaagtt gaaattaaag aagtcaatag taggttagac aatatggaac
     2761 ctgatattaa agacttaaaa actggacaaa ttgaaactag tagtagactt gatactattc
     2821 agagtgatgt tacagaaatt aagtcagatg taaaaattaa cagaaataca ttagatacag
     2881 tgattaaaca tacctcttca ttagtaaaag atacatcaga tattaaaaat gatttaaata
     2941 atatagagat agtcacatct aagaattcga atgatattgc taaattaaaa tcagttaaat
     3001 aatataccat agtaaaaaat acctgaaaat ttcacatact tctttaaagt tataccaaat
     3061 tttatacaga aatactgatt atccgacaac acactttact tataaataat ttattaaaaa
     3121 atactcattt tatcctaaaa ttaacacgac taaatgagtc ttgatataat aataaaaaat
     3181 aagggagatt gaacactaaa aagttgtgta tctcccttat attctataat atagttatat
     3241 aatttttcag cctaaaataa aagtccatct ttgattaaaa cacatcccct tatcacttat
     3301 aaaaggataa aatcttgttt tcatttttcc atttatatct tcttgcttaa gagtaataat
     3361 tttttctttt gatactttaa gtgtttggtt aaatttaacc aatcgactaa gttttgttca
     3421 acatctattt ttcatatttt ccaatactta atctattttt ataaataatt cttttatttc
     3481 taactcactt acatttttta cataaaacga agcatataaa tttcaacctt atcttgtatg
     3541 gttcttaata cttaatgagt agcttaagta tcaaacatta taaattttcc ccttacattt
     3601 aacactctta ccatttctaa aatatacaaa atatattttg aacatttgta caatgatgaa
     3661 atacaataca tgaaaataca atatcataac tgttgtctaa aaatagaagt tcttcaacat
     3721 aacctttcac aaaaaacata ttatctaagt attttttgtc tgctccttgt tttcctgttt
     3781 gtagcatata aattactgca tctaaacata taactactca cacgaaaaaa gaaaataaac
     3841 ttccaaaata caagctctaa aaactacctc taaagttgta taatgtttac taggaataag
     3901 gttgtataac atatagttta aataatcatt ttttaaaatt gcaagttatt gctttcaaaa
     3961 ttagatgttt gtatatcaaa taaatgatta agaatttcta aatttaatgg ctttataatg
     4021 cactcccttt ctttgggata aaataatgtt gcacagatac tttttctaaa gataacaatt
     4081 ttatttttct atcaattcca ccagcatacc cagtcaaact cccatttgtt cctacaacac
     4141 gatgacatgg aataataata gagattccat tatgccctac tgcaccaccg attgcttgtg
     4201 ctgacatttt agatattcct ttttgttgag caactttata tgcaatttct ccataagtga
     4261 ttgttttacc atatggtatt tgttgtaaaa ttttccacac tgtagtctga aaaggagtac
     4321 ctaccatatg gagtgatggt gtaaaattgg gttcttttcc cgaaaaataa atatctaacc
     4381 atttctttgt ctgctcgaaa attggcaatt ctttttcttg atgttcttgg tcaagattat
     4441 aagcataata cttttgattc tcaaaccaaa gaccagttaa cccaatatta tctgatgcta
     4501 aaagaatatt tccaattgga ctatggtaat agctaatata ttgcatacaa ttcctcctta
     4561 tattctgaaa acttcactca aatcattaga cacttctttt ctatctacca ttatgtaaaa
     4621 acatactaat ataatttcct tctaactaca ctttcgttat aatatctatc ctcatatcat
     4681 attagtttta tatttacctg catctgttca tccttttttc cctttccctt tgatgaatat
     4741 aaaattcata catttaagct tcctactgta ttaactttta tagaagtgta tcttataatt
     4801 ttatcactaa ataggccaaa aaaatatcga tatagcatca agtaatatca taatactttc
     4861 aaatgggaaa agtaagaata ggctgtaaaa aagaaaagta tttttatgtt agaaatccag
     4921 taaaatcatt agtagataga ataacagaaa acgaacaatt tgctatttat cattttacac
     4981 ataaatataa ttggcgcaat aaagtatttt ctattctaca acgattaata aggttagaaa
     5041 aaagtaaaaa tgaactgtat atgtatgaat cattagtttc tgtttctttc ctttggctta
     5101 ttatgataaa aaatattgtt ttgccaccaa taaaacacaa aaatataatt aatatccata
     5161 tgcaaaaaat tttacgttat attgaaaaat actattgttt tacattatat tgaagggtac
     5221 tattgtgaaa acttaatttt ggagtactat caagcagtgg taatatcaat aaatcagagt
     5281 gctccagatg atttaaacta agtttaaata ctatacctta taaatatttt actgagtttt
     5341 gtttattaaa agcaactcaa tttctaaaag gcacaaatga gctaataagt gacattacta
     5401 taaaagttgt ttttcatcaa ataatctatt ttagtaaatg ttttaaagaa aaacgggata
     5461 ttctccaaaa gtgtacagaa atataaaaaa agagatatga ttttttattt cttttgatta
     5521 tccaaagagt aaataaaaaa tattgtaaaa aacttactaa caacggcaat gctttgaact
     5581 atggtaatac tttgaacttt tgattggact ttttttgcta aaataatata tttaaatagc
     5641 aaagtaatct gtttaatcaa taaaaaataa gagataaaag aaatcaacta aaacttttac
     5701 ctcttatttt gatagtttat cagagctaat atttacactc tttttatttt tgatacttca
     5761 ttaaaaatga tgcttattct cttttcattt ttaatacttc attagaactt atgcttattc
     5821 tcttttcact tttaattctt cattaaaaat gatgcttact ctccattcac ttttaattct
     5881 tcattaaaac tgatgcttgc actctgtaaa ctcataaaca tccattaatg cttcaccaaa
     5941 tctttgatag tgaactactt ccctctcacc caaaaatctc aatatatctt tcaaatcagg
     6001 gtcatcagtt aaatttatta attgatagta cgtagccaat gctttttgtt cagcagccat
     6061 atcctcatgt aaatcagtta ccgaatcgcc aaagacatta atataacttg tagtaaagtt
     6121 tactccatta gaatctactg gctgaagacc atgtccaaaa ttagcataat tactaccaag
     6181 tccagcagcc ttaagctctt ctggagtaca attgcctatt aattgatata acactgaaga
     6241 aattatctca acatgagcca tttcctctgt acctatatca gttaaaagtg cacgagattt
     6301 tccagttggc atagtatatc tttgttgaag atatctagtt gcagctgcca actcaccatt
     6361 aggtcctccc aactgagtca tgatatattt tgccattcta gggtctgctt gtcttatgtt
     6421 aactggatgt tccagtgttt tttgataaat ccacatatta tcttactcct ccttaatata
     6481 tatttaataa taaatctaat tttaaagttt aataatttta tttataaaat gatttgtccc
     6541 atggccaagg ttcagatatc catgaccaag gatattcact tgattcatat ccaaaattag
     6601 ttaatgctcc atatttagat tcataatcac acacagcttg tgtaaattta tcgcagtatc
     6661 tgttgtatga atcaagagct ttcttatccc ttggattgtt atctaaatat aaatttaatt
     6721 ctatacaggc aaattgatat tctgaaattc tctctaaaag ttctcttcta tttaatgaca
     6781 ttagtacatg ttaccccctt ttttagctct aactctattt ttaggatttg catatagcca
     6841 atcattatag ttatttgact ctattaaatc taattcagga aataaagtac ctctttttaa
     6901 tgctgttttt ggattgtatg tgtcatcata aatttgacga ttgatgtatg gtctagcaaa
     6961 ttcacaacaa tagtcttgtt tatcctcttt taaatatccc attaattatc ctccttaatt
     7021 tttagaattt taaaatagca ttatttttaa aaattgtata cttgttctat attataactg
     7081 gtagttttaa taggtcttta
//
LOCUS       DACXMZ010000202         6861 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.203, whole genome shotgun sequence.
ACCESSION   DACXMZ010000202 DACXMZ010000000
VERSION     DACXMZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.203"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            119..1861
                     /locus_tag="JD016_15915"
     CDS             119..1861
                     /locus_tag="JD016_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAU5001103.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYRELSSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(2025..2219)
                     /locus_tag="JD016_15920"
     CDS             complement(2025..2219)
                     /locus_tag="JD016_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001104.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2216..2797)
                     /locus_tag="JD016_15925"
     CDS             complement(2216..2797)
                     /locus_tag="JD016_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAU5001105.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2965..3783
                     /locus_tag="JD016_15930"
     CDS             2965..3783
                     /locus_tag="JD016_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAU5001106.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4793..5614
                     /locus_tag="JD016_15935"
     CDS             4793..5614
                     /locus_tag="JD016_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HAU5001107.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(5924..6703)
                     /locus_tag="JD016_15940"
     CDS             complement(5924..6703)
                     /locus_tag="JD016_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAU5001108.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         2563 a          911 c          877 g         2510 t
ORIGIN      
        1 attttttata ttatttataa tcttatctat atctttatgt taaattttct taacatgcat
       61 tttttatatt taatatagta aaataattaa taactatatc catgattgga ggatttttat
      121 ggttaaatcc aagaagaaaa tatttgtttt tctaatttta attgcagttt ttattatatt
      181 tgcatcatac ataagaaatt atactaatga tacaaaaaaa gattctgtta gaggaactat
      241 tccttttgaa aaaatggaat ataagcgtcc taatatacag tcaatttgcg aaaacattaa
      301 caagtgtaac gacaaacttt taacagctaa aacagctaag gaacaattaa atttatttaa
      361 tgaagttgat aaaatttatc aggattttta cagtacactt actatagcaa aaattagaaa
      421 taacatagat tcatcagacg aattttactc taaggaatat aagtatctta tgagtaaatc
      481 agtagatgtt gacatgtcat acaaagactt tcaagataga tttgtaactt ctaaattctc
      541 taaagaatta gaaaaagaac ttggaaagga tacatttaat tatctaaaga acattggaaa
      601 acttaattct aaagaagttg aaggtttgct taaaaaagag caagacctag ttgtaaaata
      661 tgaagattta ttagctaaat ctacagtatc aatagatggt atagaggtag actttgaaga
      721 agccttgtca agaccaaatc ttagccctga agaatatgtg aaaatttatt cagattactt
      781 aaaaaaatac aatcctatat ttggtaacat attcttagaa cttatacaga caagaactga
      841 aatagcaagt aagcaaggat ttaagaatta tatagattat gcctatatga acctaaacaa
      901 agactattct caagaagaag ctaaaaaatt tagacaagat gtaaaagatt acatagttcc
      961 attatataga gagctttcat ctaaaccatc tgattcttct atttatatta aagtatataa
     1021 aaatcgctca tttaggaagt ttgataaagt acttgaagat atatctccaa aactaaaaga
     1081 gagcttcgac tatatgaaaa aatatgactt gtacgattat tcttctggaa aaaacaaatc
     1141 acctggagga tatactactt atataaataa atataaagca cctttcttgt ttaatacatg
     1201 ggacaattct tttttaggtg ttactagttt tgctcatgag tttggtcatt tttataatta
     1261 ctacaacagt attaacttaa ataataagat gcaaccaagt atagatgtat gtgaagttca
     1321 ttcaacatct ttagaaattc tattttataa atattttgat gatttttttg gtaaacaatc
     1381 tgaagctata aaaaaagaac acttatcgat tgttttaaat actattattg atgcttgtct
     1441 ttatgatgaa tttcaagaaa ttatatacaa gaatccatat atgagtctaa atgatatcaa
     1501 taaattgttt tttgatttag aggaagaata tggagtttcc aataatatac ttagagaaaa
     1561 aaatgctcct ttttggatac ttgtatctca taacttccaa gttccttttt attacttgag
     1621 ttatggtctt gcatctgatg tatcacttca gatatggcaa ttatcacaag aagattacag
     1681 aaaagctgtt gatgtatata tggatttttt aaatcaaaat actgatgctg gatttaaaga
     1741 tgtggtagaa aaggtaaatt tacaattacc tttccaaaat ggaaatttag aggaaataag
     1801 ctctacttta tatgattatt ttggtataga taatccactt gaactaaaaa atgcttctta
     1861 atttctaata ctttatactc attcttaaga tttagtattg gattgaaata agtgatattt
     1921 tttcacacac aattaatgtt taataaaatt atatggtaaa gtatcagaaa tttttaagac
     1981 tcttgttttt ataatttctg atactttatt tatcaataat attattattt ttttatatat
     2041 ataattaaaa ggattgtgga aatcaataaa aatatccctg taaaaatagc actattcata
     2101 tggaacttaa tagaacttgc atctgttacc ttattattct taatccagaa cccacaaacc
     2161 atagttgaaa gaaaaagtaa aacagtgata agacttgaac caatagttat aattttcatc
     2221 ttacatcaac atcctctcca ataatatttt ctattaaaat atcaatgaat ttatctcgct
     2281 catatttatt ctttatattt ataccactgt ataactgaaa tgattctgta gataaagcag
     2341 aaatctgtat tggtaaaata atttgcaggg caatgattct tagactcatt tcatcttttt
     2401 tatttccaaa aattttacga agcataggga caatatcaag ttctgcttgc atatctccat
     2461 tggttataca ttgattcaac acaaaaggaa gcactttttc ataatgtaat ccagtatcac
     2521 acaatttttt taataaattt ttcaaatctt caataggtct tagagtatat acactatcgt
     2581 catatagctc tgctattata tttgacatca catctcctat ggcagtactc aacaaattat
     2641 cctttgtttt aaaatgataa tttattaaac caactccaac atttgcacgc tcagcaattt
     2701 ttctaactgt aatctcctca atatcatcta cttcatttaa aagattcaat gtaacttcaa
     2761 ttatacgttc tctagcatct ttatcattac ttttcataat agacctccaa aacaattaac
     2821 atttttggtt ctgaacatgt tcagtataat atttttttca tcatatgtca atataattta
     2881 attataattt tagttgactc taacattatg atatggttta tattaatatt aagctaaaag
     2941 tacctttata aaggagacaa aaacatgttt aagataggcg atttttcaaa gctaagtaaa
     3001 attagtattc gtatgttacg tcattatgat gaaataggac ttttaactcc ttctcatacg
     3061 aataaaacta atggatatag atactatagt gcagaccaac tttctactac aaacagaatc
     3121 catgcactaa aagatatggg ctttggcttg tattctataa aagaaatttt gacagaatat
     3181 aacgataaag aaagtctcat aaaatatcta aatatccacc attctcaagt aaaagaacaa
     3241 ttagaagata cacagaaaaa actattaaaa atagaaacca ctattaaaag aattggagga
     3301 aatgatatta tgaaaaatta tgatgtaact ataaagaatt ttgcaccaaa atatatgatg
     3361 acacttagaa gggttatacc aacttatcaa gacgaaggaa tgttatggca tcaagcattt
     3421 ttagagacaa aagaccagaa tgttcaaatt gaacccccaa agtattctaa agcagttttt
     3481 tatgatactg gatataaaga agattatgta gatgttgaag tacaagttgc tgtatctgga
     3541 aagtataaag atactgaaca tgttaaattt aagactgttc caagtgtgac tgctgcaact
     3601 gcaattgtta atggaaattt caatcaagtt gcagacgctt gtgaggctat tggtaattgg
     3661 atatcagata ataactatga tgtagatggt cctatgttta atatttatca tgtaagtcct
     3721 ggcaacgata gtaatcctga taattgggta acagaggtat gcttcccagt taaaaagaag
     3781 taatttttat attaattgga taatatttta atttgttaga caaattactt ttagtgttat
     3841 gtataaatac ctacaatttg attaggatat tatcttaatt tgttaaacaa attactttta
     3901 gtgttatgta taaatatcta caatttaatt aggatattat cttaatttgt cagataaatc
     3961 acttgtgatg ttatgtttga atgactagaa tttaattagg atattatctt aatttgacag
     4021 ataaatcact tgtgatgtta tgtttaaata cctacaattt aattaggata ttatcttaat
     4081 ttgacagata aatcacttgt gatgttgtgt ttaaatgact agaatttgat taggatagta
     4141 tcttaatttg tcagataaat tatttataat gttatgttta aatacctaca atttaattag
     4201 gatattatct caatttgtca gataaatcaa ttatgatttt atgtttaaat gactagaatt
     4261 tgattaggat attatcttaa tttgtcagat aaatcaatta taagattata tactctcctt
     4321 atactctagt taaatatatt ttaatcacta atataaagta gtttttttaa caaaacaata
     4381 aaagagtaaa gtacaaataa ttcctttgtg ctctactctt tttaactttc tttagacaat
     4441 gatataatga taaatataaa aaattatata ttccattcta tagaaatatg ctcaccaata
     4501 acatgtatgt tgttaatcaa tatattatat caaattatat caaaattaca taaaccttat
     4561 aggacaatca tatcttatta caagaaaaat gtcttatcca tattgaataa gtcattctgt
     4621 tcatatacaa agtaagtttc attgataaat atttaatata tgcttataaa aatttcatac
     4681 tattctttca tatttacatg tgatataatt tattaactca taattttgtt aatcagttgt
     4741 gcttgtatat tatttatata atctaatatt ttaagaaggt ggtgtaataa ctttgaaatt
     4801 aaaaattgtt ggtagtggcg gtatgtcaat tattccaagt tcattttgta aatgcaaaat
     4861 atgtgaagaa gccagacaaa aaggcggaag atatgaacga ttagggccaa gtttatatat
     4921 tgatgatata aaaatgctga tagacacacc tgaagatatt gcagtagctt gtaacagaca
     4981 aaaaatatcg gaagtaaaac atatttcaat ttctcaccat gacccagacc atgttaaggg
     5041 tattagaatt gtcgaaaaaa taggatgtga ctttatcaca ggagaaaata atccaattgg
     5101 cttttatgcg cttcctgaag taattgaaga tataaaccga acgaatctcg attgtcttaa
     5161 atattatggt gatgttataa attgcatttc tattaatgaa acttcgcatg taaagataaa
     5221 taattttgat attgatttga ttaacaataa tccagacagg aatataacct tctatgttat
     5281 caaagagaat gaaaaaaaag tcatatatgc ctgctgtaat cccaagccat ttactcatag
     5341 tgatatgtac tttgatgctg atattttaat aataagtcta gtttctgatg atggtatatt
     5401 aaatgatggt acaaaactta aagacacacc atttaaagat gaaatattta ctttagatga
     5461 gattgttgaa attaaaaact attatagaat taaaaagatt attgttacac atattgacga
     5521 aatgtgggga aaatcatatg gttattacag tgaattagaa aagaaacttg ataacattta
     5581 ttttgcatat gatggtatgg aaattatagt ctaaatcatt aagtaataag cataatgcaa
     5641 aataagaaaa cttcaattta acagtatagt taaactatgt aatatcaaca tatctcagtt
     5701 tttacaaaat atcatagtat ttatctttat aaaatttcaa gtcctacata ttttaatact
     5761 tcaatatcta aataaagtaa aatcaaaaat atattttata tacattatag acggcttatc
     5821 ggcactagat aagccgtttt gtcacaatta ttatttgctc aacaaaactt ctcataatat
     5881 ataacagatt gaatctccaa ctgatactta attaccgaaa ctcttattcc ttaatatgtt
     5941 gttgcattaa ccaaaccttc atcatcattt tttgtggcaa tagttttgct aataaatgac
     6001 tggattttgt atttatacca tacacagaaa tatccttatt tttctttgca tccttcaatg
     6061 cttttttggc aacaatgtct ggagacacta tgcctgggaa attttttgtc cctttttcag
     6121 ctacaactat acctctctta aatagatttg tactcatcca acctggacat acagcaattg
     6181 catttatccc tttactctta agttcaacat ttaatgctct tgtataattt cttataaacg
     6241 ctttagttga actataaata ttttgatatg gcaatggctg gaatgatgct tgtgaagaaa
     6301 tattcatgat atggcttccc ttttgcatat atggaataca tataagtccc aatgctacaa
     6361 cagcactaat atttaaatca atcatattaa tagattcatc tatacttaaa tcattgtatg
     6421 aacagaattt tgcaaaacca gcattgttta taaggtattt tatacataca ttttctttac
     6481 ttaaaaatgt accaatttct tttacttctt ctatttttga taaatctttt gaaaaaattt
     6541 ttaccttact tccaaattca tctactaatt gatttaatct cgccttgttt cttgcaactg
     6601 cccatatttc tgttatttct tcttcattta ttaataactt tagaaactct ttaccaaaac
     6661 cactattagc acccgttata attgcaatct tcttataact cataattatt ctcctttacc
     6721 ataagtttat acgctcaaac tgtaaactta ttttttacaa ataaaacacc ctctattcag
     6781 atgttatatt catatcattt gagatattat acttatatca ttttcaataa ttgttatata
     6841 gcataaaaaa tagtgttaat a
//
LOCUS       DACXMZ010000203         6584 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.204, whole genome shotgun sequence.
ACCESSION   DACXMZ010000203 DACXMZ010000000
VERSION     DACXMZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.204"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(619..1179)
                     /locus_tag="JD016_15945"
     CDS             complement(619..1179)
                     /locus_tag="JD016_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAU5001109.1"
                     /translation="MLKKLKKMLLITCCLFMMSSTAFAETQNSEGKSFTISGYTFMYE
                     NAPQEIKDKYKENCLDIGVEPQPNDEIFITPSELKTYSNIDMYSARSSSFTVTYHYSY
                     YKVTGDKNYTVNTSTIVKYGNSGNPVHLVQLLLKKVGYALEVDSQFGNTTLSKVKSFQ
                     GSYGLTVDGIVGSATWKKFASATNLD"
     gene            complement(1225..2208)
                     /locus_tag="JD016_15950"
     CDS             complement(1225..2208)
                     /locus_tag="JD016_15950"
                     /inference="COORDINATES: protein motif:HMM:PF05569.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HAU5001110.1"
                     /translation="MSNLILTDILRTSITSSLVICFLLLLRTTIFRIFNKGFNYYIWL
                     IVIVRLLFPFSYFTYTISDSKFQYKPILNEVDYSNTVLIIYLTIVILLAIYRFIKYLK
                     FKSLVIDLSYDIENLEINNLYNNLLKELNIEKHIKLKYTYEINTPSFFGLFDNYILLP
                     PHNYNLNELHWILKHELIHYKSKDLYIRYLVLFLKCVYFFNPFIYLLDRIIYHSCELH
                     CDEVVLKGCSLENIKSYANTILDSIERSSRDSNNYTTGLHRKSNFEKRVYDMFKNKGK
                     SGILIALILCLLSSFTYFKFDSIISKPINHSLDAPLPVNTGKEKSFTITID"
     gene            complement(2221..2583)
                     /locus_tag="JD016_15955"
     CDS             complement(2221..2583)
                     /locus_tag="JD016_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAU5001111.1"
                     /translation="MIEKIPRAELKVMMLFWDTDKVLTSRDIVNIMSEEWKQTTTLTL
                     LSRLSKRGFLEPTKKPTHTTYKILIDKKSYLEFATSDFVKNVHNDSIVSLVDSVNKEK
                     IGKDELASLRAFIKKLKK"
     gene            3826..4473
                     /locus_tag="JD016_15960"
     CDS             3826..4473
                     /locus_tag="JD016_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001112.1"
                     /translation="MNKKLEKPFVYKREYDLTGYDVEILQKYELEQAIYVYVGSSCAY
                     NMRARSSKWRYHIRTNNKSICCNIKNFIHNLELFYKMELKLSDNIINDKLYYSNIAEF
                     EEFETLEKAREVESTIISQYQFLDSINHMLKQKIILLSNKDSVLNITKNGNTNYLKVK
                     NKYIEKHKNKPIMRYHINCQFNTDGSVKSITQEFEPILELNKKNTLSRPSRVFLK"
     gene            4748..6385
                     /locus_tag="JD016_15965"
     CDS             4748..6385
                     /locus_tag="JD016_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001113.1"
                     /translation="MEQLDSKYKLKKFLMAVFRDGIGQGNNLIDNEYVRVFQNNKSNS
                     KQLELGEEFKEYSKTTFFKNIDDIVEFTFAKNIYYENTFFNLCTTDGKAGTNENLINR
                     YALGFDFDKKELGQGFNYKDIINLFTKIGLHYHILVDSGNGFHVYVLINKTNNIKLVS
                     EVTNTLINKLGADKQANLSTQVLRVPYTYNIKNTTKQVKIIHQDKNIYRYDIEKLAKK
                     YCKDVKTVGNTNTKYILDSKLPNCIVDILKNGSKDGHKNLDLQKIVVTLRLRNKSLSQ
                     VISVAREWNYISQNSLSNSELEYQVKYMYEKLKTVNFGCTGCEFNSDCWNKIESDFIY
                     SDEDTLFNMPHKHSKDLKYKNRKGVKIMTGNQLFIYNVLLNNKDRELNIDDIMELITY
                     KRKKKVKNIVMSEKTLRETLKELQHNDYITKTKGVTKLGIKDTYNVKEVRCNIDKQYT
                     ISYFVTMAVIWGIISTEELRLYTHMRYKQDLLVKDDKIKGNILRINQEELAKDLGVTQ
                     QRISNMIESLLDTKILDVWETKINDRGFMYYTYRLNK"
BASE COUNT         2683 a          691 c          917 g         2293 t
ORIGIN      
        1 ctaattagta ggtgcttttt taaaacaagt taaaaatcaa aaatagtatt agtaagcatt
       61 ggaaatgcta gattctaaaa tagaaaagta aaaaattggt gcactattta aacttatcta
      121 tatcgctttt tccgtcgttt ggttctctag ttacgataca ggggatatgc ttatattgag
      181 ttatagtact aatcagtgct taatatagtt aataaaatta tagttaccat agtttagtaa
      241 ctatgatgta tgttagttag aaacttgcat ttcatgagtt gtaatatctt aaagtcagta
      301 gttttgattt taaaatatta attgcactgt tacaaatgac tttattaata aaaaattagg
      361 cagcttaaaa aaagtttaga ataaatttgc taggctttgc taagctattc ttttaaaaat
      421 aactttacaa aacaaacaaa taaggactta taaatataga taaaaaaaga aaattgaaaa
      481 ggattataga gacttacaag gctttaaata tatattctta attgtaaaat tatgtaatta
      541 aaatatttac tttattataa aaagacatga taaacttatt atcatgtctt ttgttaaact
      601 tatttatatg taaaaattct aatcaaggtt tgtagcagat gcaaattttt tccatgttgc
      661 actaccaaca attccatcta ccgttaagcc ataactgcct tgaaaacttt taactttgct
      721 aagtgtagtg ttcccaaatt gactgtctac ttctaaagca tatccaactt tttttaatag
      781 caattgtact aaatgaacag gatttcctga atttccgtat tttacaatcg tagaagtgtt
      841 gacagtatag tttttatctc cagtaacttt gtagtatgaa taatgataag ttactgtaaa
      901 tgaacttgac ctagctgaat acatgtctat gttgctataa gtttttaatt cactaggggt
      961 aataaaaatt tcatcattag gttgtggctc tacacctata tccaaacaat tttccttata
     1021 tttatctttt atttcttgtg gtgcattttc atacataaat gtgtaacccg aaatagtaaa
     1081 gctttttcct tcactatttt gtgtttctgc aaaagctgtg ctactcatca taaataaaca
     1141 acaagtaatt agcaacattt tttttaattt ttttaacata attcctccct ataattttga
     1201 atatatttga ataccagcaa atatttaatc tattgttata gtaaaagatt tttctttgcc
     1261 tgtattaact ggcaatggtg catctaagga gtgattaatt ggctttgata taattgaatc
     1321 aaatttaaaa tatgtaaatg atgacaataa gcataaaatc aatgctatta aaataccact
     1381 cttaccctta tttttaaaca tgtcataaac tcttttttca aaatttgatt ttctgtgtaa
     1441 tccagtagtg tagttatttg aatctcttga acttctttca attgaatcta aaattgtgtt
     1501 agcataactt tttatatttt caagggaaca accttttaat acaacttcat cacaatgtaa
     1561 ttcacagcta tgatatataa ttctatcaag tagataaata aaagggttaa agaaataaac
     1621 acactttaaa aataaaacca aatatctaat gtataaatct ttacttttgt aatgaattaa
     1681 ttcatgtttt agaatccaat gcagttcatt taaattataa ttgtgtggtg gcaaaagtat
     1741 ataattatca aataatccaa aaaatgaggg cgtattgatt tcataagtat attttagttt
     1801 aatgtgcttt tctatattta gttcttttaa taaattatta taaagattgt ttatttcaag
     1861 attttcaatg tcatatgata agtcaataac caaactctta aactttaaat attttataaa
     1921 cctataaatt gcaagtaaaa taacaattgt tagatatatt attaatactg tatttgaata
     1981 atcaacttca ttcaaaatag gtttgtattg aaatttagaa tcagaaatag tataagtaaa
     2041 ataagagaat ggaaaaagta atctaactat tacaattagc caaatataat aattaaaacc
     2101 cttattgaaa attctgaata tagttgtcct taaaagtagc agaaaacaaa taactagact
     2161 acttgtgata ctggttctta aaatatctgt tagaattaaa ttagacatat atagtaccta
     2221 ttatttctta agttttttta taaaagctct caaagatgcc aattcatctt ttcctatctt
     2281 ttctttatta actgaatcaa ctaaacttac aatagaatca ttatgtacat tctttacaaa
     2341 atcagatgtt gcaaattcta agtagctctt tttatcaatc aaaattttat aagtagtgtg
     2401 tgtaggtttt tttgtaggct ctaaaaaccc tcttttagaa agcctagata atagtgtaag
     2461 tgttgtcgtt tgtttccatt cttcactcat tatatttaca atatctcttg atgtcaatac
     2521 cttgtcagta tcccaaaata gcatcattac tttaagttca gcacgtggta ttttttctat
     2581 cataatatgt tcctccatcc taatgttaaa aaaattaata aatgtaattt tattctattg
     2641 aaatagagta ggacagtcaa ttgttttttg attaatgatt tacatatgta gagtaaatta
     2701 aaagaagtat atcacttgaa aagttttaca tacgtagagc atttttatca aatctcttaa
     2761 tttttttaat tgaattatat atgtgaatat aagagtaaat caacttaaaa catgcaaaaa
     2821 attaatattt actgacatgc aactgacaaa tcaacaaaaa taggaattga taaaataagt
     2881 gtatatattg aaattgctat aatacataca aaaatggcat aaaatactaa tttttatgtc
     2941 cttttctact gacatgtaaa aaagtcttgt aagtattgga attactagat tacataggtt
     3001 ttagatgcaa aattgtcgtt ttgtaaaaaa ggctactaaa atttataaaa acaattatta
     3061 attgatataa attatcacaa aaaaagacat ctatttctat gaaaaaaaaa ttttcgtctg
     3121 catgtaaaaa tagctatagt aatgcgtacg aatgttctat tattacagaa ttatttgtat
     3181 gtaaattata ttgaaaatat ctatcagaat tagaatttta tatggataaa acagaactat
     3241 caaataggag tatatagtaa agatttatat aatatgatga cctcgtacaa gcctgtaaga
     3301 gccgtttaaa agttgcttta gtattaatta tctttgaaaa tttaaaatag gtttaaatgg
     3361 cttgttaaat atgatgtatt actattatgt ataggggggt agttatgtag ttacatgttg
     3421 ttttaaaaaa taaatttaac tatagttctt tcacttccac attacaaaaa ataaattgag
     3481 tgtgtatgtt tttaagtaaa aatgatgttt aaatattgaa aaatagagat atagtgtatg
     3541 taaaatttag aatagtgtat gtaattttag gcataaaaat atgtgattaa accttaaaaa
     3601 ccaggaatat agtgtatgca tttttaaaaa taaaaattgt aacctttttg taatctttta
     3661 ttaaaatact tactttaagt ctaaaaatta ggggagatgt aaggatttgg gaaaaataga
     3721 agatgttata atcataaata tggtattcgt aggcttaaag tcaaaaagga ggtgaaatat
     3781 aaatagattt ttagctaaat taagtaagaa ataggaggag atttattgaa caaaaaatta
     3841 gaaaaaccat ttgtatataa gagagagtac gatttgactg gatatgatgt tgaaatttta
     3901 caaaaatatg agttagaaca agcaatatat gtttatgttg ggagtagttg tgcatataac
     3961 atgagagcta gaagtagtaa atggagatac catataagaa caaataataa gtctatatgt
     4021 tgtaacatta aaaattttat acataacttg gaattgtttt ataaaatgga attaaagttg
     4081 tcagataata ttattaatga taagctatac tatagcaata tagcagagtt tgaagaattt
     4141 gaaacactag aaaaagctag agaggtagaa agtactatta taagtcaata tcaattttta
     4201 gattctataa atcatatgtt aaaacaaaaa ataattttat tgagtaataa ggatagtgtg
     4261 ttaaacataa ctaaaaatgg aaatacaaat tatttgaaag taaaaaataa atacatagaa
     4321 aaacataaga acaagccaat aatgagatac catatcaact gtcaattcaa tacagatgga
     4381 agtgtcaaaa gtattacaca ggagtttgaa ccaatattgg aattaaacaa aaaaaatacc
     4441 ctaagccgac caagcagagt atttttaaaa taatatttta agataacaac aaaatgagat
     4501 aatactacta gacaatgaca actcaactac caattgagtt tatggagcta ccaactccaa
     4561 tatcggtcta actgattaag tatctgtagt tatataataa tattgctatc aattttagca
     4621 tcttaacaat attattatac atactaagct aaaattattc aatagttgta aaagttgatt
     4681 agtcaataag tatatattta atgtagtgtt atctcttaaa aaaactagat aaggagataa
     4741 taaatatatg gaacaattag attcaaaata taagttgaaa aaatttctaa tggcagtatt
     4801 tagagatggt ataggacaag gaaataatct tattgataat gaatatgtta gagtatttca
     4861 aaataataaa agtaatagta aacaattaga actcggagaa gaatttaaag aatatagtaa
     4921 aacaactttt tttaaaaata tagatgatat agtagaattt accttcgcaa aaaatattta
     4981 ttatgaaaat acatttttta acctatgtac aactgatgga aaagcaggaa ccaatgaaaa
     5041 cttaataaat agatatgcat taggatttga ttttgacaaa aaagaattag gacaaggttt
     5101 taattataaa gatataatta atttatttac taagatagga ttacattatc atatcctagt
     5161 tgatagtgga aatggattcc atgtttatgt gctaattaat aaaactaata acattaagtt
     5221 agtatcagaa gttacaaata cattaataaa taaattgggt gcagataaac aagcaaattt
     5281 atctactcaa gtattaagag taccttatac atataatatt aaaaatacta ctaaacaagt
     5341 aaaaataata caccaagaca aaaatatata tagatatgac atagaaaagt tagctaaaaa
     5401 atattgcaaa gatgtaaaaa cagtaggtaa tactaataca aaatatatat tagatagtaa
     5461 gctaccaaat tgtatagtag atattttaaa aaatggtagt aaagatggac ataaaaacct
     5521 agatttgcaa aaaatagttg tgactttaag attgaggaat aaaagtttaa gtcaagtaat
     5581 atccgttgct agagaatgga actatatatc acaaaatagt ctttcaaata gtgagctaga
     5641 atatcaagtc aagtatatgt atgagaagct taaaacggtt aattttggtt gtacaggttg
     5701 tgagtttaat agtgattgtt ggaataaaat agaatcagat tttatatata gtgatgaaga
     5761 tactttgttc aatatgccac ataagcactc aaaggatttg aaatataaga ataggaaagg
     5821 ggttaaaata atgactggta atcaattgtt tatctataat gtgttactta acaataaaga
     5881 tagagaatta aacatagacg atataatgga gctgataacc tataaacgta agaagaaagt
     5941 taaaaacatt gttatgagtg aaaagacatt aagagaaaca ttaaaagaac ttcaacataa
     6001 tgattatatt acaaaaacaa aaggtgttac aaagctagga ataaaagata catacaatgt
     6061 aaaagaagtt agatgtaata tagataaaca atatactatt agttactttg ttaccatggc
     6121 agtaatttgg ggaataattt caactgaaga attaagatta tatactcata tgagatataa
     6181 gcaagattta ttggtcaaag atgataaaat aaaaggaaat atattaagaa ttaatcaaga
     6241 ggaattagca aaagatttag gagtaacaca gcaaagaatt tcaaatatga tagaatcttt
     6301 attagatact aaaattttag atgtatggga aactaaaata aatgatagag gatttatgta
     6361 ctatacatat agattaaaca agtagatttt tgataggatt agaattgatt ttctagtcct
     6421 atttttatgc aaaaaaacta attaataaaa atttcttttg gtaaaataat tgtacgagaa
     6481 ttgcaaaaaa aaaatggcat caaagtattg aaattaagcc gttttaaaaa tttcttttgg
     6541 taaaataatt gtacatatat atgtagtata tatatatatg tttt
//
LOCUS       DACXMZ010000204         6522 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.205, whole genome shotgun sequence.
ACCESSION   DACXMZ010000204 DACXMZ010000000
VERSION     DACXMZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.205"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            196..855
                     /locus_tag="JD016_15970"
     CDS             196..855
                     /locus_tag="JD016_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU5001114.1"
                     /translation="MRVVKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEV
                     SRTPVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEE
                     EEVFQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQ
                     GRFIVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCED
                     E"
     gene            1228..3237
                     /locus_tag="JD016_15975"
     CDS             1228..3237
                     /locus_tag="JD016_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HAU5001115.1"
                     /translation="MKKLFTSETFLEYQFISDCQISPDGGYTAFIVKKADIKENGYTS
                     QVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRNEKVKEAVKNGKQCMSYYAL
                     SLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRPIVEGLPEEEQQKKINAYNK
                     RHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAITAPMFNVVGMKISDGKILY
                     IGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGFELYQNQVILNLTDALSYGN
                     GENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGAGQTTKVDGEYIYYTSTVDM
                     DCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCLGNGLPEVYTVENGTLCKKT
                     HLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYEVGKKYPAVLAIHGGPRLTY
                     GPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQFGDIDYIDFMEFTDTVLEK
                     YTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRSIANWSGMEGTTDIGYYFCK
                     GQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDYRCYMQEAFQMFSALKIHGC
                     PTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIKKDNIGQ"
     gene            3254..5209
                     /locus_tag="JD016_15980"
     CDS             3254..5209
                     /locus_tag="JD016_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HAU5001116.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIISHTDRFKAAASQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            5225..6415
                     /locus_tag="JD016_15985"
     CDS             5225..6415
                     /locus_tag="JD016_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAU5001117.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
BASE COUNT         2442 a          781 c         1273 g         2026 t
ORIGIN      
        1 gatttaatct atagtacaat aacttgattt gcaatacaat gaattaatct atagtataag
       61 agcatgattt acagtacaat aatttaaatt gtagtacaaa agtactatca aataagaatt
      121 tctactttaa aaatattact aaaagtaatt aaaatcaaat aattcaatat aatatggcaa
      181 aagaatagaa ataaggtgag agtagtgaaa gtaaagtcaa atttagatga aattttatat
      241 cataagatta ttgaaagtct tattcgtggt gaatactctg taggtcaaaa aatattacta
      301 aatgaccttt gtgaaaaatt tgaagtaagc agaacaccag ttgtacaagc agtaaaaatg
      361 ctaaataaag atggagtgct tactattatg acaaatggta aagtgtacgt accagagtat
      421 gaatatgata tggtaaaaca agtttgtgaa acgagaacac tgattgaaac atatgcttta
      481 gaaaaaatga tgcaagaaga ggaagaggtt tttcaacaaa agctagatgt aataaaaaga
      541 tactctgata aatgcgagga gtactatcgt caagaaaaat ctgtagaact tgcactagca
      601 gatttggaat tacataaagc cattgtagaa ggggcaaata atgagatttt aaatgatgta
      661 tatgttggta tacaaggaag atttatagta gtgaactatt taattcgacc attaaaaaat
      721 cgtaattatg aaggaacagt acaagaccat tttgaaatat taaaattcat tgaagacaga
      781 gatacaaaaa aagcagtaga taaactaaga aatcatattc aaggaactat taaaagattc
      841 tgtgaagatg aataataagt tttgaagtta ctatatatac ttatctatta agttgctaaa
      901 tgtagtctaa gtataatcta attatagttt aaacataatc taaatataat ctaataaata
      961 taatctaaat atagtgtaaa taagtctaag tatgaattaa atatagatta agtatggact
     1021 aagtatagat taagtatgaa ttaagcatgg attaaatata ctttaaatta caaatattac
     1081 aaggaagcca gaaggcttcc taaaaaaata acctttataa aattttttat tttaaattta
     1141 aaatttttta ttttaaattt aaaataaaaa atgaaagagg tggtagggag tttgaaaaaa
     1201 attactaaag aaaggataga aaatactatg aaaaagctat ttacgagtga aacatttttg
     1261 gaatatcaat ttataagtga ttgccaaata tcaccagatg gaggatatac agcatttatt
     1321 gtaaaaaaag ctgatatcaa agagaatggt tacacaagcc aagtttatat attaaacaat
     1381 aaaactggcg aattaaaaca aataacgtct ataaactctg ttggagcata tgcctgggaa
     1441 gatgaaaata ctatcttgtt ccctgctttg cgaaatgaaa aagtaaaaga agctgtcaaa
     1501 aacggtaagc aatgcatgag ttactatgct ttatctttaa atggtggaga agcagaagaa
     1561 ctttttagac tccctatcaa aggtggtaaa ttgaatccta taggaaaagg tttgtatgca
     1621 gttattgatt cttatgacaa tgaccgacct attgtagagg gattgccaga agaagaacaa
     1681 cagaaaaaaa taaatgctta taacaaaaga cattatgaac atttcaaaga aattccttat
     1741 gctgtcaatg gggagggata tattagcaga aaaagaaaac gcctatatgt atacgacagt
     1801 aatataaatg agttgaaggc aattacagca cctatgttta atgttgtagg tatgaaaata
     1861 agtgatggaa aaatcttata tatagggcaa gagtttaaag atgtaaaagg tttgaaaaat
     1921 ggggtctatg tatttgatac aaacacaaac acaaatgtct gtatattaga aaaagataag
     1981 tatattataa aaggttttga attatatcaa aatcaagtga tactaaacct aactgatgct
     2041 ttatcttatg gaaatggaga aaatggagat ttttatacta ttgatattga tacgaaagaa
     2101 atgaaacttc tttcagggca tcaacatcat tgtattggaa atacagtaac atctgatgtt
     2161 aaaatgggtg ctggtcagac aacaaaggta gatggagaat atatttatta cacttctaca
     2221 gtagatatgg actgcattat tgaaagaata catattccaa caggaaaaca ggaaaaagta
     2281 acacagacag gttcagttga ttttatagat gtaaaagatg gcaatattgt atgtgtgggt
     2341 tgtttaggaa atggattacc agaagtttat acagtagaga atggaacatt gtgtaagaaa
     2401 acacatttaa atgaccatat tttagaggaa tataaaattt ctgtgccaga atacatagag
     2461 tcaaaaggaa gttctaaatg ggaaatacaa ggatacgtat ataaaccagt agattatgag
     2521 gtggggaaaa aatatccagc tgtattggcg attcatggag gaccaagatt gacatatgga
     2581 ccatatttta tgcatgagat acaggtattt acaagtgcag gttactttgt attcttctgt
     2641 aacccaaggg ggtctgaagg tcgtggcaat gcttttgcag atattaggaa acagtttgga
     2701 gatattgatt atatagattt tatggaattt acagatactg tacttgaaaa atatacagac
     2761 attgataaaa caaaattggc tgttgaaggt ggaagttatg gtggatttat gacgaattgg
     2821 ataattggtc atacaaaccg ttttgctgtt gcttgtgctc agcgttctat tgcaaactgg
     2881 agtggcatgg agggtacaac agacattggt tattatttct gcaaaggaca aacaggtgct
     2941 tctcatatgg aaaaccacga gcttcaatgg aaacagtcac ctcttgctta tgctgataaa
     3001 tgtgttacac cgactttatt tttacatggt gaaaaagact atagatgcta tatgcaagaa
     3061 gcattccaga tgttttcagc attgaaaatt catgggtgtc ctacaaaact ttgtctattt
     3121 gaaggagaaa accatgaact gtctcgttct ggtagaccaa aacaaaagct acaaagatta
     3181 gttgaaatgc tagattggtt ttctgtgtac ataaaaaaag ataacatagg acagtagaaa
     3241 ggagaatata aaaatggaaa aaataaagat tgaaagttca gatatttttg aatatatatt
     3301 ccctcatgat atttcttgtt cacctgatgg aaagcatatt gcttatatta tatcaaatat
     3361 caacgaggaa aaagactgct atgaacatga cttgtatgtg atggacatca aaacagaaaa
     3421 acaaatacat atgactcaaa caaaagatgt aacatcattt agttggattt ctaatacaga
     3481 actccttttt acatctaaaa gaaataagcc aaaagcaggt acaacagatt tttacactat
     3541 ttctatagaa ggaggagaag caaaaaaagc attttctatt ccaaaagctt gtagcgtacc
     3601 tgtatcactt ggaaataaat tgtggttatt gacaacaaaa aatccaacgg attctaaaaa
     3661 atcagaacca gatagggcag tagagggtgt agattattgg acatttacag ataaaccttt
     3721 tataagagat ggagaaaatt tttctcaaag aagacgtgta acattagaat tatatcaaga
     3781 aggtgaaaat ataacaaaag ccattacacc aaaattttgt gaagtagcag gtgtagacgt
     3841 ttcttctgat aaaaacagaa ttttatatac tggtcagatt tatgaagatt gtgcaactcc
     3901 tttttctgga ctttgggagt atcatataga cagtggtgaa accaaagaac ttgtaccaca
     3961 aggaaagtac caaataagtt tggcaaaata tattggcaaa gataaagtaa tgttgcaagc
     4021 ttcaacatta gaccgttcta ttacacagaa tcatgatatt tttatacttg atttgagtac
     4081 tagtgaaata aatatgattg ctagtccaga tggtatgtat gctacattgc tggatgtaga
     4141 tgcagtatat ggtggtggac gtagtaataa agttatagga gacaaattta ttggtgcaag
     4201 aatttgcaga actatgacag aatttaatga gtttgataca aagacaggta atatacgtat
     4261 catcacaaaa gtagatgctt ttacatcttt tgatatttac gacaacacca tgtacacagt
     4321 aatgttgaaa gattatgagt tagcagaaat atatagcatt gatatgacta caggtacaat
     4381 gaaaaaaatg actgcttttt ctaaaccata tttagatact cataaggttt cattacctga
     4441 aaaattgacg tttgtagcaa aaaacaaaga agaagttgat ggttttgtta taccacctat
     4501 agatgctaaa gaaggcgaaa aatatcctgc tgtattgttc attcatggtg ggcctaaatg
     4561 ggcatatggc tatatgttta cacacttgaa gcagtgtgta acatcaaagg gaatgtatgt
     4621 gatttattgt aaccctcatg gtggtgacgg ttatggtgaa aaattcttag aaatggtaga
     4681 aagatggggg tatgttgact atgagcatct tatggaattt gtagatactt gtattgaaaa
     4741 atatcctggg attgatgcag accgcttagg ggttgcaggg ggaagttacg gtggttatat
     4801 gacaaactgg ataattagtc atacagaccg tttcaaagca gcagcttctc aaagaggtat
     4861 tagtaacttg attacagctt ctttaattat tgactttggg gatagaatta tgaagcagac
     4921 ttgtggagat aagacgcctt ggaatcatga ggaggttctt tggaatcatt cacctattaa
     4981 atatgtgaaa aatgttaaga caccaacatt gttcttacat agtgataggg attatagatg
     5041 ttttatgggg gatactttcc aaatgtttac agcattaaaa caacttggtg tagatacaga
     5101 aatgtatttg ttccacggag atacacatgg attgtctaga aatggaagac catctaatag
     5161 aattgcacga gcaaatgcta ttgtagattg gtttgaaaga tatttataat aagggggtta
     5221 ttctatgagt gatatgttat tatggtttag tgtcgtatta gttttatttg ctattgggga
     5281 tttaattgca agcaaaacaa aagcaaaagt atcagctgta tttgttacac tacttttatt
     5341 tttgattcta tttgtcacaa aagcaatacc agctgatatt attgaaaaag caggtatgac
     5401 cgcagcagct agttggagtg ttccaatgat tatgtttagt atgggaacaa tgttgaatgt
     5461 taaacaattt atagacgaat ggagaacagt attgacagca tggcttggaa ttgtagcagt
     5521 tatagtctgt gtctcattat gtattccttt gtttggaaaa tcaacagtat tgacaagcat
     5581 accagttata aatggagctt tacctgctac tactattatg acacaagcag cactagaaaa
     5641 aggactgaca cttgcagcag ctacagcaac agttgtattt gcaattcaga agtttattgg
     5701 tacaccaata gcttcaagag cagcgttaca agaggcaaac cgtcttttag tagaatatca
     5761 tgaagcaaaa tcaaaaggca ttgatttagc aaatgttgat actgataaga aagaagcaga
     5821 gaatacaggc acaaaagtaa aacaagcttt ctgtgaaaaa tacgataaat attattctac
     5881 aaatgtttgt atatttttta tagcattatt ctcttatcta gggtatgaac tttcagagat
     5941 tattcatgta aattattcta ttgtatgtct tgttgttggt gttattgtaa cacgtattgg
     6001 tattgtgcca aaagacattt tggaaaaagg aaaaatcaag ggatttatca acatggttgt
     6061 gtttgcagct gttattcctt ctttagcaaa agtttctttg acagatttaa ttagtctttt
     6121 tgtaccaatt gtgggtatgt ttgcagcttc tatcattggt atattcctta tgatgaaggt
     6181 gttaccaggt tggaaaatta tcggttctaa acctcttgct tttggagtag ggttttgtca
     6241 gatgttggga ttcccaacta catatttaat ttcaaatgaa gtgtgtaatg ctgttggaga
     6301 aacggaagaa gaaagagcat atttgatgtc taagattatg cctaagctgg tagttggtgg
     6361 tatggcttgt atgatttcta ttgtagtagc tggtcttatg gtaccaatgt tgtagatagg
     6421 tttcaaagtt aaaaaagtgg tattaattta taggagaagc taaaaagtag tattaattta
     6481 taaaataatt ttatattagt taatttgtta taagaatatt tt
//
LOCUS       DACXMZ010000205         6513 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.206, whole genome shotgun sequence.
ACCESSION   DACXMZ010000205 DACXMZ010000000
VERSION     DACXMZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.206"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(153..2540)
                     /gene="cadA"
                     /locus_tag="JD016_15990"
     CDS             complement(153..2540)
                     /gene="cadA"
                     /locus_tag="JD016_15990"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAU5001118.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENTSSKTSIKKELILGGLNC
                     AHCAEEINDKVSKLKEVESSNLNFVNKKLTVNISNNFKEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2563..2928)
                     /locus_tag="JD016_15995"
     CDS             complement(2563..2928)
                     /locus_tag="JD016_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HAU5001119.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3127..3975
                     /locus_tag="JD016_16000"
     CDS             3127..3975
                     /locus_tag="JD016_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001120.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            complement(4412..5500)
                     /locus_tag="JD016_16005"
     CDS             complement(4412..5500)
                     /locus_tag="JD016_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001121.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5625..6353
                     /locus_tag="JD016_16010"
     CDS             5625..6353
                     /locus_tag="JD016_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HAU5001122.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
BASE COUNT         2268 a         1094 c          717 g         2434 t
ORIGIN      
        1 ataatggata ataaatataa atttatatat gcaaagcgct ccaataaaaa ggtaccaata
       61 aagttaaaag aaacttattg atacctttca ttattacata cttcaatata taattataag
      121 ttttttaaat agtatggatt tacatttatc ccttatctca tagctctcat agcatttact
      181 actgctataa gtgccacacc tacatcagca aatatagctt cccacatagt agctactcct
      241 ccagcaccta gtatcataac tataattttt actcctagag caaatattat gttttgccac
      301 actatcttat tagtcttgtt tgctatttca attgctttag atatcttgct aggctcatct
      361 gtcataagca ctacatctgc agcttcaata gcagcatctg aacctaagcc tcccatagct
      421 ataccaacat cagcacgagc taatactgga gcatcattta ttccatcacc tacaaacgct
      481 attttctctt tttcagttct tccttcatat aaatcttcta atctatctac cttttcatta
      541 ggaagtaaat ttgagtaaac tgtatccaat tctaattctt gagcaatatt ttttgctact
      601 ttctcattat ctccagttaa cattacaact tcttttaccc ctatttcttt taagcttctt
      661 atggcatttt tactatcttc ttttacttca tcagagataa ctatataacc tctatatact
      721 ttatcaacag caatatagac tactgtgcct acttctttag ctgatgaata agatatattt
      781 tcttttttca taagtttctc attacctgct aatataaaat tgccattatg ctttactcta
      841 attccataag ctgctatttc ctcataactg tctattgtat ctaaatcaat ttttttatta
      901 tagtaactta atatggactt tgctattgga tgatttgaat ttacctccgc aatagctgca
      961 tattctaata attcttcttc tgatatacct tcaggattta acttagttac attgaacaca
     1021 cctttagtaa gtgttcctgt tttatcaaat actactgtat ctacacttct taaagcttct
     1081 aaatagttac ttcctttaat aaggatacca tttttagatg caaacccaat tccactaaag
     1141 aaactaagtg gtattgataa aactaatgca caagggcaag aaacgactaa gaatatcaat
     1201 cctctatata accaatcact aaatacctca cctgagatta caagtggtgg cacaaatgct
     1261 attaataaag cagctattac tactattgga gtatagtatc ttgaaaactt agatataaag
     1321 ttttctgtct ttgatttttt aatgctagaa ttttcaacaa ggtcaagaat cttagaaact
     1381 gttgattctc caaaactctt tgtaacttca atactcaata aagcattttt atttataaca
     1441 cctgataaga tttcatctcc tttttcaact tctctcagca ctgactctcc tgtgagtgca
     1501 gaagtatcta acatagaaac tccatctact actacaccat ctaatggtac tttttcacca
     1561 ggttttacta ctattatatc acctacattt acctcttctg gtgacaccac cttaacttca
     1621 gaattaactt ttagatttgc atagtctggt cttatttgca taagtgaagt tatagacttt
     1681 ctggatttac caactgcaac accttgtaaa tactctccta ctttatagaa aagcataacc
     1741 cctactgctt ctgataactc ccctattgct aatgctccta ctgttgctag agccatcaaa
     1801 aagttctcat caaatactct tccttttgat atatttctta ttgctcttaa aagcacatca
     1861 ccacctacta ttacataggc aactataaat actatattgc taaactgact ttcataacct
     1921 gtagctgttt gatatatacc aaatatatat acaagtgctc caattataag tggaattaat
     1981 tctttcttat ttgtatcatt tacagctcct gatttttcag tagttctccc ttttactttg
     2041 tcagtagacc ctacttgtat atcaagccct ggctctgttg aatttattat ttcttttatt
     2101 ttgtttataa catcatcctc tttaaagtta ttacttatat tcactgtaag ttttttattt
     2161 acaaagttta aattagaaga ttcaacttct tttaatttag aaaccttgtc attaatctcc
     2221 tctgcacaat gtgcacagtt taatcctcct aatataagct cttttttaat tgatgtttta
     2281 ctagatgtat tttctttaga acttatttgt atatcaagac ctggctctgt tgaatctatt
     2341 atatctatta tcttttctat agtagtatcc tcattaaagc tttcctttat atttactgta
     2401 agttttttat ttataaaatt taaattggaa gatttaactt cttgtagttt agaaacctta
     2461 ttattaatct cctctgcaca gtgagcacaa tttaaccctc ctagtataaa ttctttttta
     2521 attaaattat ttgcttccat atatattcac tcctctcatt tatcatttat aagtctctct
     2581 aatatgattt aatccacaat caaatatctg acttatatga ctatcatcta atgagtaata
     2641 cactgttttt ccttctcttc taaactttac taatctagcc tgctttaata ctctaagttg
     2701 atgagatatt gcagagtgag tcatatttaa taaactcgct atatcacata cacacatttc
     2761 atttgcaaat aatgcatata atatctttac tctagtagta tccccaaata ctttaaataa
     2821 ttctgctaaa tcatataaca tttcttcatc tggcatagtt gatttagcct ctgttactat
     2881 ttcttcatgt actatattac aattacaatc atttatctct tctctcattt ttaatcctcc
     2941 attattttaa tatatgaata actgttcata tgttcattat atgttatcat ttctaaattt
     3001 gcaacaataa tttttattaa tttaatataa atagaatgca catattataa acttttttaa
     3061 tatatatatt attagaataa gtttttgtaa acttttttat agttttctag gaggtgcaag
     3121 tttaatatga gtaacaagaa aaaaaaggat ttagatacta gttacatgcc ttacttagac
     3181 ccagcgaact atgcagatta tattactaat gaagaaattc cccaagatga attgagaaat
     3241 gaccctcaaa tttctaatgg ttatacaaaa atacctaata accagaacat aaataaccta
     3301 tatcctaata atgtaaatca ttatcctaaa agtagtaatt ctccaaacaa cttgcctaac
     3361 acaaataata catccggaaa tatgaattac aatacatctg ctacaaataa tatgcctaat
     3421 aacatgaata ataatacggg tatgcctaat aatatgtcta acaatatgaa tagtaatatg
     3481 ggtatgccta acaatatgtc taataacatg aatagtaata tgggtatgcc taacaatatg
     3541 cctaataata tgaatagtaa catgggcatg cctaacaatg ttcctaataa catgaatagt
     3601 aatatgggta tgcctaacaa tatgtctaat aatatgaata gtaatatggg tatgcctaac
     3661 aatatgtctg gcaatatgaa taacaatatg tatggtaata caggtatgcc taataacatg
     3721 aacagtaata tggggtataa tatgaataat actatgggta ttcctaactt ctcatgtaat
     3781 caaaatatgc ctcctaatgt acttatgatg ataccaggag ttatatgtca taatacaatg
     3841 caaggtatgc ctgtagttat gcctagtact atgcctccta atatatatcc tactccttat
     3901 ggttctagta atatgtctat acaaggtata ccacaggcta ctaatattga agaatttgat
     3961 gaggaagaaa tgtaatatat atttttttaa gtaagttgta taaaacatta ataattgacc
     4021 ataataataa atgtattcct cataatattc ccccaatatt attataggta tagaaagttg
     4081 actgaatatg cgcttattcg gtcaacagat attaacataa tctaataaga taaacataga
     4141 aaaagtcaga taaaatttaa ttttatctga ctttttcatt taaaaatgct ctcttgtgtt
     4201 ggtgggtcag aatagtatga tactcgtatt tgagactaat attggcaaac aacataaaga
     4261 aaagtataaa ttttttataa taaactgtat aaaattttaa taattgacca caataataaa
     4321 tgtattcctc ataatattcc cccaatatta tagaaataaa tttaagcgag attaaattca
     4381 gttaatctcg cttaaataat gtctaaattt attattccaa aaaatcatca aaatctaatg
     4441 aacctgattc tgcaaaatca tataatgcat tttgctcaaa gaaaaatttc tcttcaaatt
     4501 taatatagtc gctataatta gattgactta tttcattgat ataattgcaa tcattttcgt
     4561 tataagtacc aataaaaaac ttatttccca ttaagtaata tatagctttc atatcattat
     4621 ttattatata tacattttct tttacatggt cattattaaa attaaatcta gtaaagaata
     4681 taccttcatt catattactt tttaacagaa atggattttc tctataaaat actcctgcaa
     4741 attcttcagg tgaatttaca ctgtaaacac ttacaaattc acttttagaa atctcatcaa
     4801 atacctcaaa atcatacata ggttctttat ctgtcactaa tagagaatta attctaaaac
     4861 cagtttcata acacttactt atgcaaaaag caattttaca tatataatat ccatcattat
     4921 cttcatataa agcttcactt atatatctgc cttgaccttt atcacttacc acgtttttaa
     4981 gtaatgttcc atttatatta gtaatatgca tatttgcaat ctcgtcactt ccagaaaagt
     5041 actttaaact ttctctatcc catgctataa atctcatggt catatagttt ataaattcaa
     5101 cttcctcgtc aactctttta catattttat tgtataattt ttctatagtt aaatctgttt
     5161 tatagttttt tataatatca atatactcac atttatctcc tggcaatagc ttatcataat
     5221 aaagattctt attaccaaaa taagatacta aaaataaaga ttcatccttc gttaattcaa
     5281 ctctatctga accaaatcct cccatcaatc tttcttcttc catataagct tcttcttgtg
     5341 ttgggttatt taaactcaca taatccgcta atcctaatcc ctctgcatct aataaaaaat
     5401 attgacaaaa gtgattgttt tcatcatcta tccaatttat tataagacct aaactgccca
     5461 taagtctaga atttgtagcc tttgcaaaat taaaattcat atagtcacat cctttaaact
     5521 ctaaaatact tgttttttaa tatataaatt attatacata tattttaaca acttagatac
     5581 aatttataat ataatataag gaaatgatta attgaggtgg tcttatgaaa aatatagcag
     5641 ctttttttga tatagatggt actctttata gagactctct tatggttgaa cattttaaaa
     5701 aattaattaa atacgatata attgaccaaa aaaattggta taaacatgct agagacactt
     5761 ttatggattg ggataaaaga caaggtaatt atgatgatta tctattagaa atatgtgacc
     5821 tttatgttga ttcactaaaa ggattagata aaacctgtat caactttaca agtgaccaag
     5881 taattaaatt aaaagcagat agagtttata aatacacacg ttcaaggatt gagtggcatc
     5941 taaatcaagg acacatagtt atatttatat ctggtagccc tggatttctt gttgaaaaaa
     6001 tggcaaaaaa atataatgta accgattttc taggaagtga ttatgtcttt gaaaacaata
     6061 tatttacagg cacagttatt ccaatgtggg attctataag taagaataat gctataaacg
     6121 attttgttgt aaagtatgat ttagatttat caaactcgta tgcctatgga gatactaatg
     6181 gtgatataaa tatgctaaaa agagttggaa atccaatagc aataaatcca acaaaggaac
     6241 ttttatctca aatatcactt gatgaggaaa tttctaaaaa tgttgagata atagtagaaa
     6301 gaaaagatat tatttattct ctatctacag atgtaaatat agtggatatt taatttatac
     6361 tatctatttt aaaaacttat attatatcta atatttatat ctaacatata aaagctttga
     6421 ctaaaaatca aagcttttat atgttgcact aagaatatat taaatttagc tttatgcttt
     6481 aatgtatatt catatatatc atttacactt atc
//
LOCUS       DACXMZ010000206         6503 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.207, whole genome shotgun sequence.
ACCESSION   DACXMZ010000206 DACXMZ010000000
VERSION     DACXMZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.207"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            71..700
                     /locus_tag="JD016_16015"
     CDS             71..700
                     /locus_tag="JD016_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001123.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            701..1483
                     /locus_tag="JD016_16020"
     CDS             701..1483
                     /locus_tag="JD016_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5001124.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1476..2354
                     /locus_tag="JD016_16025"
     CDS             1476..2354
                     /locus_tag="JD016_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001125.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIVNDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2387..3346
                     /locus_tag="JD016_16030"
     CDS             2387..3346
                     /locus_tag="JD016_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAU5001126.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3662..4354
                     /locus_tag="JD016_16035"
     CDS             3662..4354
                     /locus_tag="JD016_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5001127.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4364..5368
                     /locus_tag="JD016_16040"
     CDS             4364..5368
                     /locus_tag="JD016_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU5001128.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            5613..6467
                     /locus_tag="JD016_16045"
     CDS             5613..6467
                     /locus_tag="JD016_16045"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAU5001129.1"
                     /translation="MMSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKG
                     QFAEMFEVVIQLGAILAVVVLYWKKIKDSIIEFFKFIFTGGKEGKIGFRFGMNVIIGC
                     IPFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQ
                     ALKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFE
                     FDYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVL
                     AFTNIISV"
BASE COUNT         2542 a          591 c         1197 g         2173 t
ORIGIN      
        1 gacaaaattt aagtgctcat ttaaactatt aattgataat aatattcaat attaaataat
       61 aggaggcgtt atgaataaat atcttctaat aaatccagtg gcagaaagaa tgtatggaga
      121 aaagtttcac cttattaaag caaatttgat aaaaaagggg tacataatag cagaatgtga
      181 accacagtta gaatatgtaa aaaagcagta caaagagtat acaaaaaaga ctgaaaatac
      241 aatgttggat tgtagatgtc cagaaagtat aagtctttta aaaagaaata atttgataaa
      301 tgattttgaa gtacctatga tagaaccaat attagtaagg acttgtaggg ttttatacga
      361 taaatatata aaaagtaaag atgatatgtt ggtagtaacc tgtccatgta cacaattaag
      421 agactttgca agtgaaaagt ttaaggataa gggtaatgct atgttttata catggaaaga
      481 gtttgctgaa caagaaggtg taaaatcact tggaaaaata gatgaatcac caattccatt
      541 aggatatttc aaagatactt ttgaaaaagt tcttgaagta tcaagtgaag acgaaatatt
      601 aagtgaaata caaaaaataa gaaatggttt tgaagataag tatgacatag tagaaatgtt
      661 ttattgtaaa gatggttgta ataatggaga tggtttgtaa atggtcaata aatttaataa
      721 taagttttta aggctagtaa agagtgttct gatatttttt ataattcttt tattatggaa
      781 aattacaaat tatcttggta tttggagtga ttatatatta ccatcacccg aaaaggtata
      841 tagtacattt ttaaatatga taagtgatgg ttcaatattt attaatgtat atgcaagtat
      901 gaaaagagtt ttgataggtt ttgcaataag tactgctata ggtgtgcctt tagggatatt
      961 ctttggaatt tatagtggag tatacgaata ctttaaatct ctaataaatt ttttaaggaa
     1021 tacacctccc cttgctttaa taccaatgct tatattatgg tttggtatag gagaggaatc
     1081 aaaaattata attatagttt tagcatcctt tttcccaatc tttacaagca cattgaaagg
     1141 gattaaaaat tgtgactcaa aactgattga agtgggtaga gtatttgagt tttcaaagct
     1201 tcagataata tttaaaataa taataccaaa tgccattctt gatattgctg ttgggttaaa
     1261 gttagctctt ggatatagct ttagagcaat tattggggca gaacttgtag cagcttcatc
     1321 tggtcttgga tacttgatat ctgatggaaa agaaatgtct agaacagatg ttgttattgt
     1381 aggtataatc gtaattggac ttttaggaat aattacagat tacatatttt cgattattgt
     1441 gaagaaggta agtaagggaa agatggtgga agcatatgaa taatttagta tgtgactctg
     1501 gatatataat tagaaattta tataaaagtt ataaagttga caatgaagaa catgtagttc
     1561 ttagtgatat ctctttaaac atagatagaa accatataac agtaatttta ggtgaaagtg
     1621 gttgtggaaa gactaccttg cttagagcaa ttgcatcact tgaaggtata aatagtggtg
     1681 aaattaggtt tattaatgat aataaagagt atagacctaa tgttggtttt gtttttcagg
     1741 agagcagatt aatgccttgg ttaaacgtaa gtgaaaatat agcttttcat aatcaaagaa
     1801 aaaatactat tttaaacaag gtgcttagaa aatttaaaca caataaaaga attagcagta
     1861 aaattgtaaa tgataatttt agaagcttag aaacaaacga taatttaaac aaatcaaata
     1921 aatataatag gaaaagtaat attgatgtag aaaaatatct taagatgatg aacttgcaga
     1981 aatttaaaaa ctcttatcca aatgaattgt caggaggtat ggcacagaga gtaagcatag
     2041 ctagagctct ttctttcaat ccagatatgt tgcttatgga cgagcctttt tcggcattag
     2101 attattttac aaggattgat atgcaaaatg aagttataag aatacataag tcaactaata
     2161 aaggcgtgat atttgtaact catgatattg atgaagcgtt aaagataggc aagaaaatag
     2221 ttgttttcac agacgaaaga aaagtaaaag aatttaatat agaagatggt tataatagag
     2281 atttaacttc agattactat attaaattaa aaaaagatat tttaatgaca atgaaatcaa
     2341 ataaaggcat ttaaagaaat taataatatt ttaaggagta aagtaaatga aattaaaaaa
     2401 gctattgata ggtataacga tattaacact tgcaacagca tctttagctg gatgtgctaa
     2461 aaaaaataat ggagaaaaac tttcagaaat aaatttaact tatgtaaaat caccattaaa
     2521 tgttccttct ataattcaga aacaggatga tttatttgga aaggaattta aaaaggataa
     2581 tataaaagtt aacttccacg aaattactac aggtccagag caaacacaag ctttagctgc
     2641 tggagaaata gacttcttac atgcactagg tggtacatca gctttaatag ctgcatctaa
     2701 tggagtagag ttaaagatat taaacactta tagcaggtca ccaaaaggat ttatgatttt
     2761 gactaataat aattcaataa aatcagcagc agatttagta ggtaagaagg tggcaggccc
     2821 aaaagggact atattacatc aggtattgat ttcagcactt gataaagaag gattaagtat
     2881 ggatgatgtg gaatttgtta atatgggtat tccagaggca tcagcagctc tttctgatgg
     2941 cagcgtagat gctgcattga ttgctggtcc agcagctctt aaagctatga agtcaggtag
     3001 taaacttgta gcaaatggtg aaggtttagt agatggtata atagtaactg ctgttagcac
     3061 agactttgca gagaaacatc cagaaatagt tgaaagattt atgaaggttg aaaaggaaac
     3121 tttagaatat gtaaataata attttgatga agctatggaa aaagtagcta aagaagttga
     3181 tttaagtctt gaagaaacta aagaattata tgcttggtat gattttagtt tagatataac
     3241 agataaagac atatcttcgc ttgaagatac acaagacttc ttaataaaaa ataagttaca
     3301 agagaaaaaa gtgaacataa aagaattaat ctataatgct aagtagaaac tatgataaaa
     3361 taatactaag taagaaatat aataaaagtt taagttacct tattaaggta actttttcta
     3421 ttttgaatta gtaatttcaa tttattttag aatgattagt tgataataat gattaattaa
     3481 tcataaaaag atactttttt atagagtttt attaagatgt ctaataatat ggttatactt
     3541 atgaagtcag taattgtgaa gttagtaata tgattaaatt tatatattgt aaaaagatta
     3601 taatatatta aaattggact gttaatatga tgagtaaata ggtatatata ggaggaatta
     3661 tatgagttat aaagtatata ttgtggaaga tgatatatca ataagtacat tacttaaaga
     3721 ttatattgag aaatatggat ttgaagtgat tgttgaagaa aactttgatg atataatgat
     3781 tacatttgaa agttttaagc cacacttagt tctattagat gtaaatttac ctaaatttga
     3841 tggtttctac tggtgtagat gtattaggca acagtcgaat ttacctataa tttttatatc
     3901 agctagagat ggtaatgtag accaaattag agctttagaa agtggagcag atgattatat
     3961 aacaaaacct ttttactatg atgtagtaat ggcaaaaata aagagtcata taagaagggt
     4021 atatggtgaa tattcaccta aaatagacga aagaatagtg gagctagatg gattaaagtt
     4081 ttatccagag aggttagaac tagaattaaa tggtgagaat ctaattataa ctaaaaaaga
     4141 aggtatactg attgaatgtc ttatcaagag atacccaaaa gtagtaagta gagattatct
     4201 tttagaaaag atatgggatg atattgagtt tgtagaggaa aatactttaa atgtaaatgt
     4261 tagcaggata aggaaaagat ttgaggaatt aggtatagat aatgctgttc agactgtccg
     4321 agggttagga tataaattaa ataagaattg gtaaggagaa agtatgaagt tatttttaaa
     4381 agattataaa ggttatattt ttatatatta tataggcata gttatgacaa ttatttactg
     4441 taatcttatg aagtttatta gttttggaga gagtttatat attcttttat ttagttcttt
     4501 tatattgtta tgttttttaa tatataagta ttataaaaat agagaagtat ataatctgtt
     4561 tgaaaaagga gtacattctt tggaggagtc atttttgtat ttaggaagct cattcttggg
     4621 gagaaatata tcagttattt taaaacaaca acataaattg tatcaatcaa tgatacaaga
     4681 gcataaaaag atacatagtg aacatttaac tttcatcaac caatggattc atcagatgaa
     4741 aacaccaata tcagttataa gccttcagct tcaagattat gagggagaag aagtaactga
     4801 aaatataaga aaagaaatag atagattaaa taaagggtta gatatggcta tgcattttgc
     4861 aagggcagat aattttcaga aagattttat agttgaaaaa gccaaattaa atgaaattgt
     4921 atctagtatt gtaaatgaag aaaaaaggtt atttataaaa agtggaatga ttcctaagat
     4981 tcaagtagac aaatttactt atgtttatac agatgtaaaa tggatgaaat ttataatagg
     5041 tcaattaatt acaaatggag tgaaatattc taaagattat agtaactacc taactataaa
     5101 atctgaagaa aataataaat atgtaatttt aagtataata gatgaaggaa ttggaattgt
     5161 ttctaaagat ttaaaaaggg tatttgaccc attttttaca ggagaaaatg gtcgtaagtt
     5221 tggagaatct acaggtatgg gtctttatct agtaaaaaag gtatgtgatg atttaggcca
     5281 taaagtaagt ataaaatcag aagtaggaaa aggtacagaa gtttcaatct actttaataa
     5341 agatgtaaat agtttaataa aggtgtaaat tatctgttac aatagggttt ttagttaatt
     5401 ctactttttt attatacaat ttattaaatg aaatattcat agttaaataa tcaaaattta
     5461 agagagttta tttgcaaacc ttacatagat gtaaggtttg tgtaatgttt gcagatagtt
     5521 aaattttcta atctaaatta caattaatat aagaaataac ataagagaaa taacataaaa
     5581 aaagaaattt tgtaaaaaag gaaggatgtt tattgatgag tttagatgtt atttttatat
     5641 tgaaatcagt cataatagct atagtagagg ggcttacaga atttattcca gtttcatcta
     5701 caggtcatat gattttggtt ggtaatttaa tagattttaa ggggcaattt gctgaaatgt
     5761 ttgaggttgt aatacaactt ggggctatat tagcagtagt agtactttat tggaaaaaga
     5821 taaaagatag cattatagaa ttttttaaat ttatatttac tggcggaaaa gaaggaaaga
     5881 ttggttttag atttggaatg aatgtaatta taggttgtat accatttgcg attataggag
     5941 ttctatttta cgataatata aaatcattgt ttaatttaca atctgtaatt ataggattta
     6001 tagtgggtgg tattttatta ttagtagtag aaactttgtt tagaaaaaag aatcattcca
     6061 cagataatat agataaaatt actccaatac aagctttaaa ggttgggaca ttacaagttt
     6121 tgtctgcatg gccaggtatg tcaaggagtg catctactat tatgggtgga tggatagcag
     6181 gacttaattc tcctacagca gctgagtttt ctttcttttt agcagtacca gctatggtgg
     6241 cttcttcagg aaaagattta tttgaatttg attattcaat aatgacacca acattgtgga
     6301 ttgctttggt agttgggttt atagtagctt ttattgtatc aataatagtt atggaaaaat
     6361 tcgtgaattt tttaaagaaa aaaccaatga gagtgtttgc tgtatacaga ataatcatgg
     6421 gtgtagtact tgcagtatta gcttttacta acataattag tgtatagaaa aaatagattt
     6481 attatctata aaaattaaac taa
//
LOCUS       DACXMZ010000207         6499 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.208, whole genome shotgun sequence.
ACCESSION   DACXMZ010000207 DACXMZ010000000
VERSION     DACXMZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.208"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(16..1383)
                     /locus_tag="JD016_16050"
     CDS             complement(16..1383)
                     /locus_tag="JD016_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAU5001130.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(1633..2070)
                     /locus_tag="JD016_16055"
     CDS             complement(1633..2070)
                     /locus_tag="JD016_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HAU5001131.1"
                     /translation="MGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVYVFSVIGSTL
                     VSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGIILFVGIPLPT
                     TGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(2102..2731)
                     /locus_tag="JD016_16060"
     CDS             complement(2102..2731)
                     /locus_tag="JD016_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HAU5001132.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(2765..3652)
                     /locus_tag="JD016_16065"
     CDS             complement(2765..3652)
                     /locus_tag="JD016_16065"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HAU5001133.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(3681..4919)
                     /locus_tag="JD016_16070"
     CDS             complement(3681..4919)
                     /locus_tag="JD016_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001134.1"
                     /translation="MDGDILMSKILKYKNEMLLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLILHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(5303..6445)
                     /locus_tag="JD016_16075"
     CDS             complement(5303..6445)
                     /locus_tag="JD016_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAU5001135.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
BASE COUNT         2314 a         1085 c          669 g         2431 t
ORIGIN      
        1 tttatattgt atatcttatt cttttattgc tttaggtttt gatagaaaac atttataaaa
       61 tataaagaaa gaaattgctg ttatagtagc agctgttata tctgcaactg gctctgctaa
      121 aattactgca aaaagttttt cattaaagaa tgttgggagt ataaatatta aaggtattaa
      181 aagtacaatc tttctaagta gagctaaaac caaagatatt gttgcttttc caagtgctaa
      241 aaatgtctgt tgacaagcta tttgaatccc aaacataaaa gcaccagcaa aatatatttt
      301 tatactccaa ctagtcatac taagtaactg cggattctta ttaaatatac taacaaaaaa
      361 tgcaggaaat atcattaata aaagccataa aatagtagaa tatgtaaaac atgacaataa
      421 acacaactta aatgtctttt ttactctatc aagttgacct gaaccataat tataacttat
      481 tattggttgt gctccttgtg taacccccat attaggaagc ataagaattt gcattatact
      541 gctcattatg gccattgaac ctactgctaa atctcctcca tacatttgta atttactatt
      601 taaggctata agtactaaac tttctgtaga ttgcattata aatggagaga ttcccaagcc
      661 aagcactggt aagatgatac ttgcttttgg tatcatatac ttctttttta ttttaagtat
      721 acttttcttt ccaaagagaa acataagtac ccaaatagca gaaacgcctt gtgcaattat
      781 agtagctaat gcagctcctt ttacacccat attaaagcca aatataaaaa tcgggtcaag
      841 tattatgttt attaaagcac ctatagcaac tgtcatcatt ccagttgtag caaacccttg
      901 ggtatttata aaagtattca tacccattga aatctgaaca aacactgtac ctattaaata
      961 aatatttaaa tagtccattg aatatccaat tgtagattca cttgctccaa acatccaaag
     1021 taaaggctct tttccaatct taaaacctat agtcaaaagt attgctaaaa tcaccagtac
     1081 agaaaaactg ttactcatta ttttctctgc ctcatcattg ttttgctcac ccatttttat
     1141 agcagctaat ggacctcctc ccataccgat taaataacta catgcagtta ttataattat
     1201 tattggaaat gccactccaa caccagccat agcaacttct ccattttcca ttctaccaat
     1261 aaaaatccta tcgactatgt tgtataaaac atttacaatt tgtgcgataa tagcaggtgt
     1321 agcaagttta aataataatt tacccacact ttcactacct aagttagtgt ttgaattttc
     1381 cattttaaaa cctcctcaat ccaataatta aaatgtatca ctaaaatttt attctatata
     1441 aaaatttcat tttattagca gacaaattta gagtactttc cctacaagta atactatact
     1501 atatttcttt tgccatagta aacattttag aactaactga agttaaatac tttatctcat
     1561 tttttattta ttattaaaaa tttttcacac atactcataa aaaaactatc caagtatatt
     1621 acttagatag ttttatatta tatgtaaaga aagagctgaa actataaccc ctgataacaa
     1681 attgccaaga aatactccca taaaagcatc ttttattctc atttttaata tggaagctat
     1741 agcagatgca gtccatgttc ctgtagttgg cagtggtata cctacaaata gtattatacc
     1801 aattatcgaa acacttttta atttcttcat tcttgagttt atttttctat caattacatc
     1861 agcaatccct ttaaatagtt tcttccctct taaaaattgt aatacatgtc tgaatgtcaa
     1921 tatcaaggga atcgatacca atgtagaacc tattacacta aatacataaa cccatatagg
     1981 atttaagccc attgcaatac ctataggaat ggctcccctc aattctgtta caggaaccat
     2041 agataaaatt aatatgtgta aatatcccaa actattccct cctattctaa gtgacattaa
     2101 gttatgaatt tatttgctct ataacctcac catccttttt actaactaaa aatgcatttt
     2161 tagtcaatga aagcattggt aaatcactct tagagtctga atatgcatat gaattttcat
     2221 aatcaatctc tatcccaagt ttacctaata cagcctttat tctttttact ttttcaattc
     2281 ccttacagtt ttctccaatg aattgatttt tatatcttga attttcataa aaaaactccg
     2341 tgcctataac ctcgtctgca agaccataat acttaaaata tttcatatat gcatatggag
     2401 atgcagttat cataattata acattatctt cttctttatt tttgtaaata acctttttca
     2461 tagattcgtt aatttttttc attatgcaat catcaaaaaa cttttttaaa tcttcttcac
     2521 taaaatctac tattggataa aagattgcct cttttgcctt cttcaagggt atcattctaa
     2581 ttaaatacat aaatagaata aaaataatgt atggcaactt tataatagtt ttaaaggttt
     2641 tattttttaa agagtaaata aaaaaatcta tcatgctatc agtatttata acagtataat
     2701 ctacatcaag taataataca ctttttttca taagtatttc cccatcctat cactaaaaat
     2761 atcactaaaa aatcaatatt attaaacatg taattatcca taagattacg tttattttta
     2821 aagcattatc ttttaggaca atatcagtag gattattttc atttgtagtg ttaattatat
     2881 aattatatct aagtacacca taaactacaa atattgttgt aaatatcata tgtggctttt
     2941 ctggattact tgtagaatac aatgcataac atacaattgt agaacttaat ataactatca
     3001 tcatttgatt tagattttct atgtcataat cctccaatat ttttctatgt tcaactgcat
     3061 catctcttag agtttctatt tcttttttac gttttccaag agctaagtac agagataaga
     3121 aaaatgtaca taaaattatc caatttgata aactaacact tatggctaca catcctgcat
     3181 aaaccctaag tataaaacca agagatattg aaaacacatc taatagtatt atattcttta
     3241 gtttaagaga atataatata ttgtttatca aatagagact gagaattata gaaagattaa
     3301 aactaacaac tgttgataaa cagactgaaa ttaaagctaa tactacaaat agtatattag
     3361 cacttttttt gcttattctt ccacttgcca ctggtctatt acatttttta ggatgttttt
     3421 tatctttatc catgtctata gcatcattca gtgcatatac tgatgatgac attaggcaaa
     3481 aacacacaaa cgcaataaac gtatttaaaa ataaatttat atttagtatg ttgtttgaaa
     3541 aaattatagc tgaaaataca aagaaattct ttatatattg ctttattctc attaacttta
     3601 tataatctga aattttagta agaagcctta tacttgtatt ggtctgtttc ataaacttac
     3661 taccctttca atattatttt ttaaacagga tatgatttta tccctatgtt taaaatttct
     3721 ataaatccat tgacattact gattactata gtaaccaata acagcgatag aaatatatta
     3781 tacatattct tttcattttt tctaagcgct ttcgtaaagt aagccaataa aattataact
     3841 gcaaaactaa aatttatagc cataatactt gaattgacta gattatatcc tactatacca
     3901 tgtaaaatta gattgaatat aattattccc atacatagct taggaactct tttttctgaa
     3961 ttttcaataa ctgataaaac acaaaaacat aaagctaata atgtaagaat tactggtatt
     4021 gtagcaactt ctccattttg caaaaataac ccttttttaa tacttgctcc aggaaaataa
     4081 aaaaccgaat acaataatct ctctataatc attgcaatct tttccaaaat tggttctcca
     4141 cttataaatt tcgtatcata ctgtattaca tttccaaggt tcataaaact ctcataagac
     4201 ttcgttgaaa ctacacaaat atatgccatt aggataaatg taattaaatg ttttaacatt
     4261 tttcctattt ttaatttaaa ctcaaatatc atattaaaaa aatacaaata tatattacta
     4321 atagttattc caaatagagg aatagctgct aattctacta aataagaatt ttttcctttc
     4381 atcttatttg caataactat gaaaaatatt aatgaaaatt ggctatatat aaatctctct
     4441 acatttaaag tcataaataa ctgtggaaaa gaaaatatca ttatcatagt aagtaaaatt
     4501 aaagttaatc tctgcatttt tatcttttta atatgattaa agactaaagt tattgacata
     4561 gcactagtta ttatttgtag acaaattagt ccaaacccat aggcactatt aaaatctgtt
     4621 gtcccaggat ttgctattga agtacaaaat aggtatattg gatatgcaaa aattgatata
     4681 attgctgaaa acaatatgtg cttactattg tcttgactta tagcaaatac atttaaattg
     4741 aataaaactc ctgtatctgt atctaacaat acatcataag tattgtttaa atcataaaat
     4801 atagtagtct tagaaaaaaa tatcttagtt attattaagt aagttattac aactaatata
     4861 aacaaaagca tctcgttttt atattttaat attttactca tcaatatatc accatccatt
     4921 cattatatag gatttgctga cacctacaat ttattcctta tatttaatag ccatcttcat
     4981 aatgttctta cttgttttat tgtaattgaa taactcttta tttacaacca caatatttac
     5041 tttattgatg ttataatcag tcaagtattg tttatataac atagattagt gttgtataaa
     5101 atgagaattt gtaagcaata ttaatataaa atatttgaaa aactttatat aaaaatatac
     5161 aatgttgctt atggaattaa actataacaa attaaagcat aatttaagga gacaaaatta
     5221 taatctgtct ccttaaatat taaaactata ttttaaaaca ttactaaata ctatacaaat
     5281 ttcaaatata gtataaaatt aattagactt taacactatt agctccatct tcttcccatt
     5341 ttttaatttc ttttaatcta tctattatag gtaaatgctg agtacattca acttcacatt
     5401 ttccacaaga tatacattct ttagcttttg ccttactatt cattgtgtaa ttccaatgtc
     5461 ctaagccata aactaacttt gtcatttctt catcactctt tttaaacatc tgcttttcat
     5521 tataaaaaag catataggct ggggtattaa taccttttgg acatacttta caataaccac
     5581 aacctgtaca tatttcgttc atattcttat ttaatctagt ctcaatatca tgtatatcct
     5641 catctgaata aattctattt tcgtctgcaa ttttacatgc atcatctata tcttgttttt
     5701 tattaaaccc tatcaaagat atacttattt gtgaaattcc tatgttgaat cttaaagctg
     5761 attctatagt actttcgcca tcttttgata gaaaagaaag ttctttatta tactttggaa
     5821 tagttccacc acttaatgga ttcattgcca caacaccata tccattatca acagcatatt
     5881 tggcaccttt tagcctatat ggaaagttta atatatttat tcccatagtt acaccttcaa
     5941 acttattttc acttagaata tctataacct catctcctgg ttgatgagat gaaaatacta
     6001 tatggtctat caatccttct tctttacatt tcaataatcc ttcatattga cctccaggtc
     6061 tcattgctag ttcgtaatgt tccatctttc taatattcca aacatgataa aaatttattt
     6121 tagatacgcc taatcgttct attgattttt ttacagcctc tatagctttt tctgatgtat
     6181 cacatgcttt tggcttttct ttagttgata cataaaaatc ggtcttacct tcctttatca
     6241 tttgtctaaa ggctagtcca aatatatctt cacttctgtc atcacaatac cctggtgctg
     6301 tatcaaagta attaatacct ttttcatacg catatttgac taaatcagca ttttcttgat
     6361 tacttttttc taaatcaaac cttaatcctc catagccaac tactgaaacc attttccctg
     6421 tctttccata ttcaacgtat ctcattgtaa attcctcctt agatatatta agacttaatt
     6481 tctttataga aaatattat
//
LOCUS       DACXMZ010000208         6330 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.209, whole genome shotgun sequence.
ACCESSION   DACXMZ010000208 DACXMZ010000000
VERSION     DACXMZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.209"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            292..1236
                     /locus_tag="JD016_16080"
     CDS             292..1236
                     /locus_tag="JD016_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HAU5001136.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1258..1884
                     /locus_tag="JD016_16085"
     CDS             1258..1884
                     /locus_tag="JD016_16085"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAU5001137.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1918..2967
                     /gene="hisC"
                     /locus_tag="JD016_16090"
     CDS             1918..2967
                     /gene="hisC"
                     /locus_tag="JD016_16090"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAU5001138.1"
                     /translation="MREKESIRELRGYEPNHVNCEVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEI
                     SMFVGAIRDILKKEK"
     gene            2970..3548
                     /gene="hisB"
                     /locus_tag="JD016_16095"
     CDS             2970..3548
                     /gene="hisB"
                     /locus_tag="JD016_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HAU5001139.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3550..4155
                     /gene="hisH"
                     /locus_tag="JD016_16100"
     CDS             3550..4155
                     /gene="hisH"
                     /locus_tag="JD016_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAU5001140.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLRLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4152..4874
                     /locus_tag="JD016_16105"
     CDS             4152..4874
                     /locus_tag="JD016_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAU5001141.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4856..5614
                     /gene="hisF"
                     /locus_tag="JD016_16110"
     CDS             4856..5614
                     /gene="hisF"
                     /locus_tag="JD016_16110"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAU5001142.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5642..6310
                     /locus_tag="JD016_16115"
     CDS             5642..6310
                     /locus_tag="JD016_16115"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAU5001143.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2582 a          535 c         1255 g         1958 t
ORIGIN      
        1 taaatttaga atattaataa aaattatttg ggaggaaatt atcatggtga gattaaatat
       61 agtaaaatta aatacaaaat taaatcatca ccatcatcat agattaaagg gattgtaata
      121 tgaaattaat tcatagacac aagccctgtt tttagtgtaa ttggtcttgt atctatgatg
      181 ggaagtaatt ttaagagaaa attatagcca tataggtata aagcctatat ggctttttta
      241 ttgttttaaa tttgcaaata taagagtaaa tttaatgaag agggtgagaa tgtgaaattt
      301 ttgaggattg aagatgaggt tatttattct aaaaaaagat ataaattaaa aagggaaata
      361 gataaacttt ttttggatga agaatatttt caaatagaac cacaattatt tgaagaatat
      421 gatgaattta caactataaa taataaaatt ccagaagaat ctatggtaaa ggttgtaaat
      481 ggaaaagtaa tggtacttag agctgatata actacaagta taataaaaag ccttgtacca
      541 agatgggaag atggtttaaa gcttaagctt ttttataatt catcaatata taaaaataaa
      601 aatacagttg gaataaaaga gataaggcaa ataggttgtg agtatctagg agaagcctct
      661 gtagaagcag atagagaagt agtgaaatta gctcttaaga tacttgaaaa atacaataat
      721 aattttattc tagaggttgg gagtagcaag tatattcatg gtttgttaga agaattaaat
      781 ttaaataaga attgtgaaaa tcaaattaaa aatttactat atacgaagaa cactcatgaa
      841 ttaaaagtat atattgaaga attgaaaatt aagcgtgaag taaaagagtt attatcaaat
      901 attttaagtt tacaagggaa tttatataat gttattgaaa aatcgaacaa gttctattgt
      961 aataataaga tgaagcaagc tcttgaagaa ttaaagcaag taaataattt aattgaggaa
     1021 tgtaattttt tagataaggc tagatttgat ttatcaatga ttactatgct tgactattat
     1081 gaaggaatta tgtttagagg atactaccca aactcatata aagaaattct tagtggcgga
     1141 agatatgatt ctttaactaa agagtatgga aaagaaattc cagccattgg atttacacta
     1201 agtgttgatg agttaatgaa gtatgtatat aagtaaagtt tagaaggagg gattgttttg
     1261 gattatttga ctatagcact agcaaaagga cgaattgaag gggaatcatt taaaaaattt
     1321 aaaaaaatgg gtctaggaga tagtatagac acagatacga gaaaactaat ttttaaagat
     1381 gaagaaaata aaattatata tatacatgta aagccatcag atgtagttac atatgttgaa
     1441 aaaggagtcg ctgacctcgg tatagcaggt aaagatacta ttttagaaaa tgagacagat
     1501 gtatatgaaa tatatgattt aggttttgga aaatgtaaat ttgctgtagc aggattaaag
     1561 ggagatagca tatatagaga agatgaatat ttaaaagtgg caacaaagta tcccaatata
     1621 gcaaaaaagt attttaaaga gaaaggtcaa aaaattgaaa taataaaatt aaatggttct
     1681 gtagaactag ctccaatagt agggttgtca gatgtaattg tagatatagt tgagactggc
     1741 aatacactta aagcaaatgg tcttgaaata ttagaagaca tatgtaatat aagtgctaga
     1801 attatatcaa atagagctag ctatagattt aaatatgaac aaattcagaa tataattcgt
     1861 ttatttgaag aacttgataa ttaaaatata attgagcttt ataaggaggg atttaagttg
     1921 agagaaaaag aaagcataag agagttaagg ggatatgaac caaatcacgt aaactgtgaa
     1981 gtaaaacttg atgcaaatga aggaagtaag aggttgttta aataccttat taaagaaatt
     2041 tcagatagtg atatagattt aaatttatat cctgaagatt cttatagtga tttgaaagaa
     2101 tctataatag attacataaa tatttctgga gtaaataaga aaaatcttct agtaggaaac
     2161 ggttcaagtg aaataattga tttaataata catacttttg tagataaaga tgaagtaata
     2221 ttgagtttct ctccaagttt tagtatgtat tctatatata gtcagataaa tgggtctaaa
     2281 tttataggtg ttgaaagtga tgaaaattta gttataaata tagataatgt gattgaaaaa
     2341 gtaaaggaga ataatcctaa aatagtaatt gtatgtaatc ctaataatcc aactggaaca
     2401 atattaaaga gggaagagat aataaagctt ttagattcaa ctaatagtct agtggtttta
     2461 gatgaagcat atatggattt tggagaagaa agcatgttaa gtgatgtttt taagtatgat
     2521 aatctaatag ttcttagaac tttatcaaag gcatttggat tggctggaat aagaacagga
     2581 tatatgttat ctaatagtag tttaataaat tctgttgaga aagtaagacc tccatataat
     2641 ttaaattcat tatcagactt tattgctaca agagcactta gaaataaaga tgtggtaaag
     2701 gcttatataa aggaagtaaa agaggaaaga gaagttttat atgaagaaat gattggtatg
     2761 ggaattaaag catacaaatc acaagcaaat tttatattat tttattctga aatcgaaaat
     2821 ttatcacaaa aacttattga tagaggtgtt ttaataagaa aatttggtgg aaagttagaa
     2881 aattattaca gagttacaat tggtgataaa gaagaaattt ctatgtttgt tggagctata
     2941 agagatattt tgaaaaagga gaaataggta tgagaatttg gaaggtagaa agaaatactc
     3001 ttgaaacaca gattttagta gaattaaata ttgatggttc tggtaaagct gaaatagata
     3061 ctggaatagg atttttagac catatgctta ccttaatgtc atttcatggc aagttcgact
     3121 taaaagtgat ttgtaagggt gatacatatg tagatgacca ccatagtgta gaagatatag
     3181 gtatagctat aggtgaagca tttaaaaatg ctctaggtga caaaaaagga atacgaagat
     3241 attctaatat ctatatacct atggatgaat ctctatcaat ggtagcaata gatataagca
     3301 atagacctta tcttgtattt aatgctaaat ttgataccca aatgattggt agtatgagta
     3361 ctcaatgctt taaagaattt tttagagctt ttgtaaatga atcgagagta acattacata
     3421 tcaatctctt atatggggaa aatgaccatc ataagataga aagtattttt aaagcatttg
     3481 caagagcttt aaaagagggt agtgaaatag tttctaatga gatagcatct tcaaaggggg
     3541 ttttgtagaa tgaatataat agtagactat ggtcttggaa atatagattc tgtgtctagg
     3601 ggttttagaa aagctggaat agaaactaaa atttctagtg atatagatga aataaaacag
     3661 gcagattcac ttatacttcc aggtgttgga gcttttagag attctataag tgccttagat
     3721 aaactaagat tgataccaat aataaaggag catgtttcta agggtaaatt tatgataggt
     3781 atatgtctag gaatgcaatt actttatgaa aaaagttatg agtatggaga atatgaagga
     3841 ttaggactta taaaaggaag tattgataaa ctggatataa gtttaaaagt gccacatatg
     3901 ggatggaata atctaaaatt taataaggca aatgatgata tacttaaata tataaatgaa
     3961 gatgattatg tatacttcgt tcactcatat tatgcaaatt catcaaatga agaactaatt
     4021 gcattttcag aatatgaaaa gaaaattcct gctattgtta gaaaaggcaa tgtatatggt
     4081 atacaatttc atcctgaaaa aagtggagaa gtagggttaa atatactaag agcatatggg
     4141 gagatgataa aatgataata tttccagcaa tagatataaa agataataag tgtgtaagac
     4201 tcacacaagg agaatttgac aaagtaaatg tatattatga taatccatta gaagtagcat
     4261 ataaatggaa aaatgaagga gcagaatata tacatatagt agatttaaat ggtgctagaa
     4321 gtgaatttgg tgtaaataca aaaataattg aggacatagc aaacaatata gatataccta
     4381 ttcaagttgg cggtggagtt agggataagg aaaaagtcaa aagtttaata aatgctggtg
     4441 ttactagagt aatattaggt agtatagcga ttgaaaattt gaatttagtt gaagaattgg
     4501 tcaatgaata taaagagaaa attgtagttt ctatagatgc taaagatgga aaagtagcag
     4561 ttaggggatg ggaagttgta agcaatgtag actcattaac attatgtaaa caacttgaaa
     4621 agataggtgt acaaactatt gtttatacag atatatcaaa agatgggatg ttgcaaggtc
     4681 caaattttga catatatgaa agaatagcaa aggaaacatc actaaatgtt attgcttctg
     4741 gaggcgtaac ttctatagaa gacgtaaaaa gactaaaagc tatgaactta tatggtgcaa
     4801 taataggtaa agcgctttat gataaaaaga tagactttaa ggaggcacaa caattatgct
     4861 tactaggaga ataatacctt gtctagatgt gagaaatgga agagtcgtaa agggaaaaaa
     4921 atttaaagat atagtagatg ttgatagtcc agaagtactt ggaaaatttt atagtgattg
     4981 tggtgcagat gaacttgtat tctatgatat aactgcatca aatgaagaga gaaagacatc
     5041 tttagaattt gtgacaaaag ttgcagaaaa tataaatata ccattttgtg ttggtggagg
     5101 agtaaataaa ttagaagatt tcactgatat tttaagaaaa ggtgctgata aagtttctat
     5161 aaattcatca gcagtcaaaa atcctgaatt aataagggaa gcttcattaa aatttggagc
     5221 acaatgtgtt gttttatcga tagatgcaaa gaaaaatgaa gagggttcat ggagcgtata
     5281 cgtaaaaggt ggaagagaaa aaactaatct ggatgcaatt gaatgggctg ttaaaggtgt
     5341 agaacttgga gcaggagaaa tcgttgtaaa tagtatggat gaagatggaa tgaaaaatgg
     5401 atatgatata gaattattat caaagataac ttctttagta aatgtacctg ttatagcatc
     5461 tggaggcgct ggaaaaaagg aagattttta tgaggcggtt aataaatcca atgtagatgg
     5521 aattttagca gcatctgttt ttcattttgg cgagataaaa ataaacgatt taaagaaata
     5581 tttaaaagac atgggtgtag aagtaagatt ataagttaaa tactaaaagg agattagaaa
     5641 tatggataat aagtgtaaca atgtatacag tgatgaagta gagaaattta taagaagtat
     5701 aaagtttgat gataaagggt tagtccctgt tgttgttcaa gaggtagtta gcaaagatgt
     5761 actgatgtta gcatatatga ataaagatgc tataaagaaa accttaaaag ataaagtagc
     5821 ttgctatttt agtagaagta gacaagaact ttgggttaaa ggtgagacat ctggtaatac
     5881 tcaaaaagta gttaagatgt cttatgattg tgatgtagat actatactac tttttgttga
     5941 acaaactggt gtagcttgtc atacaggcaa ttattcttgt ttttacagag acttgtttga
     6001 tgatagttca aaaatggagc ttgaagtaca aacaaatata ttgaaagaat tatatgattt
     6061 aatcaatgaa agaaaaaata atccagttga aggttcttat actaattatc tttttgaaaa
     6121 aggaatagat aagattctta aaaaagtagg tgaagagagt agtgaggtaa ttatagcatc
     6181 aaaaaatacc gataaaagtg aacttattta cgaaattagc gatttagtat atcatacatt
     6241 ggttttaatg atagaaaaag gtgttgaaat tgatgaaata aaaaaagaat tacttaaaag
     6301 aagaaaatag aataagatag attaatgggt
//
LOCUS       DACXMZ010000209         6325 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.210, whole genome shotgun sequence.
ACCESSION   DACXMZ010000209 DACXMZ010000000
VERSION     DACXMZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.210"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            124..3312
                     /locus_tag="JD016_16120"
     CDS             124..3312
                     /locus_tag="JD016_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAU5001144.1"
                     /translation="MNNMKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDL
                     QNNLLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVA
                     IVIKSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVN
                     LDVLINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFA
                     DEDEKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEY
                     HPKILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDY
                     SKIYQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYF
                     DMIDGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDL
                     FTFLDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPK
                     EYKDILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKAL
                     YINDVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKV
                     LDYYKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIK
                     VLLIHGNKRDREKCFMKLENFDVILTTYGTLRNDLDKYSEIKFDYCILDEAQNIKNPV
                     ALVTESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDN
                     VKNLKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDK
                     NLKKDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFS
                     QFTSVLKNISKELDKYKIKYHYIDGKTNAKERLKLVDEFNNSMDKKVFLISLKAGGTG
                     LNLTSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQES
                     KKELINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(3597..4361)
                     /locus_tag="JD016_16125"
     CDS             complement(3597..4361)
                     /locus_tag="JD016_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAU5001145.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIINFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4517..4750)
                     /locus_tag="JD016_16130"
     CDS             complement(4517..4750)
                     /locus_tag="JD016_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001146.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(4824..6299)
                     /locus_tag="JD016_16135"
     CDS             complement(4824..6299)
                     /locus_tag="JD016_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAU5001147.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATINEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVVIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNVLVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKILFEEYSKG
                     ILKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGITNDDIGV
                     IEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
BASE COUNT         2355 a          900 c          735 g         2335 t
ORIGIN      
        1 gtaaattgtt atttacacat atattgtgat agataaaagc tattcatatc tgccttaatt
       61 ttagtcttaa atttagagtg taaattgaat gcatttagat tataatatat acagtgaggt
      121 aaggtgaata atatgaaaat agatatgata tataaactaa taagaagaaa tacagaacca
      181 tcaagatggt caagaggtgt aaaatattat actaatcaat tagttgatta tatagacata
      241 gatattgatg acctacaaaa taacctgttg acatttcgtg gtggagtcaa ttctgaagat
      301 aattatagct tttattttaa ttcaataact atagatataa agcactttaa gataattgaa
      361 aatgattgta gttgcgctga ctgtcaatca aagggcaaaa gtatttttat atgtaagcat
      421 atagttgcta ttgtgataaa aagcttaaaa caaataaaaa ataaaaaaga tgttcctcct
      481 attctaatag atatattagg tgcagataag gataagcttt ctaatacaaa ttctaaaacc
      541 agcttagatt taagcaagca attactttct ttgttagaca atactaaaag agaaagggtt
      601 aatttagatg tattgataaa tgtggattca aataaagcaa ttgctgaatt taaaatagga
      661 attgataaaa tgtacatttt aaaaaacatt actgaatttg catattcaaa acttaatgga
      721 tatgaattag attttgggaa gaaatttact tacaacccca acactcatta ttttgctgat
      781 gaagatgaga agttagtaga tattatagaa gaatatggga caaatctttc atacaacttt
      841 tcttcatcac attcaaaaaa atttatggag ataaatgcaa gctctttaaa acaattttta
      901 aatgctatta agtacaagga ttttaccctt attttttcta aagaggaata tcatccaaag
      961 atattaaatg atactctacc tgtaaggttt gattttaaaa atatagaaga taaaatagtc
     1021 ttatattcta aagattcact acctgttcca cttactaaaa aaaatgatgt acttttatat
     1081 gatggcaata tctatttatt atcacataaa aaagctcatg attattcaaa aatatatcaa
     1141 atattatcaa agactaaaga gttaaaattt gataaagagg attctaagga tgttttagga
     1201 ttgctagttc ctagattaaa atctatatct caagaagttc atttagatga taacataaaa
     1261 aataatataa caaaagattt taaatctgag ttctactttg atatgattga tggcaacata
     1321 tgttgtgatg taaagtatat atatgatgat gaagataaaa aatttgtgct ccccaacata
     1381 caaaaggaag ccactattga aaataaactt acttcttcac aatttacaaa ggaaaataat
     1441 cattatgtgt ttaaaggaac tgaccatgac ctatttacat ttttagattt acaattagaa
     1501 tctctaaagg aatttggaga tatatattat tctgagaagt ttaaattgaa aaaaatatat
     1561 aacgcttcat caattcaagc ttcaatcaat cagactaacc agaattacct agaatttaat
     1621 tttaatatat ctgatataaa tccaaaagag tacaaggata ttttgaaagc ttttcaagaa
     1681 aaacgtactt tttataaact taaagatgga aactttatag atttatcaga aagagaaaca
     1741 aaagattttt ttgaattagt tgaaaatcta gatataatgg ataaatcaaa aaataataaa
     1801 attcataaaa ataaagctct atatataaat gatgtaatac gtagtaaaaa tttaaagttt
     1861 atagatggta aaaaagaact agacagtata tgtgaaaaat ttagaaattt tgatagcata
     1921 aatttagaaa ttcccgcaaa tttaaatgca aaccttcgtg actatcaaat aaatggcctc
     1981 aattggttta aagttttaga ttattataaa tttggtggta tattagctga tgaaatgggt
     2041 cttggtaaaa caatacagac tattgcattt ttattatcat tatctaataa aaaaagtctt
     2101 attgttacac cgacatcact catttataat tggaaaaatg aatttgaaaa gtttgctcca
     2161 gatataaaag ttttattgat acatggaaat aaaagagata gagaaaaatg ttttatgaaa
     2221 ttagaaaatt ttgatgtaat acttacaaca tatggtactc ttagaaatga tttagacaaa
     2281 tatagtgaaa taaagtttga ttattgtata ttagacgaag ctcaaaatat aaaaaatcca
     2341 gtagctttag taactgagtc tgtaaaaagt ataaatgcag aaaataaatt tgcactcact
     2401 ggtacaccta tggaaaataa tctactagaa ttatggtcta tatttgattt tataatgcct
     2461 ggctatctat atagtaaagc aaagtttcag gaattgttta taaataaaga agataatgtt
     2521 aaaaacctga aaaaattgat aaaacctttt attcttagaa gaagtaaaaa acaagttatg
     2581 aaagaacttc cagataagat tgaaaaaaac ttttttgttg aattgaataa agagcagaaa
     2641 aagatatata gtgtatattc aaaagatata caagataaaa tgaaagataa aaatctaaag
     2701 aaagataaaa tagttatatt ttcttattta actaaactaa gacaactttg tttagaccca
     2761 agtatagtag taaaagatta caataaaaaa agttcaaaaa ttgaaacatg tttagaaata
     2821 cttagggata gtataaacga aaaccacaaa atattattgt tttcacaatt tacttctgtg
     2881 cttaagaaca taagtaagga acttgataag tataaaatta aataccatta tatagatggt
     2941 aaaactaatg ccaaagaaag acttaaactt gtagatgagt tcaataatag tatggataaa
     3001 aaagtttttt taatatcttt aaaagcaggc ggaactggtt taaatcttac atctgctgac
     3061 atggtaattc actttgaccc atggtggaat ccatcagtag aaaatcaagc tagtgataga
     3121 gctcatagat ttggtcaaaa aaattcagta caagttataa aattgattgc taaagggact
     3181 atagaagaaa aaataataaa attacaagaa agtaaaaaag agcttataaa tcaatttata
     3241 aatggagaac tttctaatga aggtgtatta aaaagtctat ctgatgagga gataataaac
     3301 ttatttaact aaattattta atcctacagc ttaactatat aaatttgttc taacatttat
     3361 atagttaagc taaaatatct gaataaataa aatttaatat tgttatttaa tcccaacttt
     3421 acaatctaat gatatctctt tctatacata ttgcatatac aaatgtaata acttgactat
     3481 tagaatattt tgttttaaca atactatttc atcataataa tgaagtatat gtacactcac
     3541 ttttgtaagt tttgcaatcc attttacagt tttcattatt gtaaattttg gttaaattat
     3601 tcctctaaaa atttctttgc tacatttgaa aaatctaatc cattcgataa aattgcagga
     3661 tgaccacctt tatcaaacaa atacatttta ccatttttaa tcttttcaag aagtaatgaa
     3721 aaagtatttt caaaaaaatc actagatatt agagatacaa actcatcttc tctacttcct
     3781 gttaaaagca cctctggttg catagtttct agaggcttat gaaaaaactt tcctatagtt
     3841 ttataatgtt caaaaatagc ttctgtatca ttatctacaa ctttttccca gttttctcct
     3901 tgattataaa tataaaaagc ttttgcacca tcatcttgct tagatgcttc acgctctatt
     3961 ttcacatttt gtacaaatga ctctagagga acttctcctt caaaactatc tgcaattacc
     4021 ttattcacta aatcaggtct ctcaagtgca acatttaatg caacaagagc tcctccacta
     4081 ctaccaatta tgtttacttt tttatagttc attgcttcta aaaaacatat cacttgcata
     4141 gcctcatcaa accataagtc agcagaaaat ttatcgacca tttgagattt tccatgacct
     4201 acaaaatcaa ttaatattac tttatattca tctttataaa aattgattat atcattaaac
     4261 atcttggaag aggctgtatt tccatgtaaa aaaagtaatg gtgtaccatc cccaatttca
     4321 ttatagtagc actgtttgtc gttatagtta aaatatgcca tatatttccc ccctttaata
     4381 tccctatagg tatatattta ttacttatat ttaaaatgac tattccctta gccacttaaa
     4441 aatagtataa cttagaatgt agataaaatc aacattcctt gctaatttat ctattgtttg
     4501 tctaatttat tataatttaa cttctgtaaa atttcttttt tgtacgaaaa tattccttcc
     4561 atttacaaca atcattcaaa tgaagtataa catgacgagt aggaggcata agcaaaattc
     4621 cagcaatatc tttattcccc caaaagtcca acaaccaaat agaatcttca tgctttatta
     4681 ctcctccagt caccaaaatc ctttctaagt caattggaga gacctttcgt ttcatatctt
     4741 cagctttcaa atccttaaca acttctattg tgttttttgc aacttcattt cgataacaaa
     4801 gcaattcatc aatattgact aaattatcgt atatctcttt tcactttaac catttttttc
     4861 tttataggta tctccttata tttttttaat atcaaatctc ccttataatt taatatatct
     4921 acatatgaac ataaatcttg aacttcaatt acacctatat catcattagt tatacctttt
     4981 atattactaa atgcaccaac tatatctaaa actcttattt tcttcttctt accagcattt
     5041 aaatataatt taacaacttc actttctata tttagtttta tatctttact tttgtaagaa
     5101 tttttgtcaa ctttttttag gattcctttt gaatattctt caaacaaaat tttgccatgt
     5161 gtaattctac ttttttctat atcttttaat tcatttattt tatatcctat atacgtttct
     5221 atattctcta tatatttttc atccttttca gttactatag taattgcctt tccactattt
     5281 ccttttctcc cagttctacc tattctatgt atatagtttt ctttatcttg tggtacatca
     5341 taatttatta ctaaagaaat atcatcaata tgtattcctc tagacgctac atcactacta
     5401 actaatacat taaatttttg gtttttaaaa tccttaatta caaaaattct tctttcttgt
     5461 gataaatctg catgtaactc tcttattaaa aaaccctctt tactcatctt attatataat
     5521 ttagatactt tctctttggt attacaaaat attaccacac tctttggatt ttcgctgtat
     5581 attaattctt ttaaaaggat gtacttctct cttccatttg ccttgattat tttgtcatct
     5641 atttgtcttg ttttttgatt agtatcgctc tcattttctt ctatatttat aacactgtaa
     5701 tctagcatat atttttcaca tatatatttt atctcttcat ttattgtagc tgaaaaaaga
     5761 cttactattt tctccttagg taaatttaat aatatttttt ccatttgttc aacaaaacct
     5821 ttattaaaca ttttatctgc ctcatctatt actaaatact ttacattttc caacttaata
     5881 gaacctctat ttatatggtc aagtattctg cctggagtag ctacaacaat atgaaccctt
     5941 tgtttcaatt cagctatttg gtcttttata gattgtttgc caaatatagc actacatctc
     6001 accttcttta gtctccctat atctgatatc tcatctttta cttgcaaggc taattctctt
     6061 gttggaacca ctataagagc ttgaatatta ttgtaatcta catttatatt ctcgcataaa
     6121 ggtattgcaa aacttgctgt ctttccactt cctgtttttg acctaactac taagttttga
     6181 ccttttaata attttggaat aacttctctt tgtactctag aaggtatatt atatcctaaa
     6241 ctcttcaatg acttaagtat cttttcattt aacttaaatt tttcaaatgt atatttcata
     6301 tattaccacc ttattatatt tatct
//
LOCUS       DACXMZ010000210         6276 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.211, whole genome shotgun sequence.
ACCESSION   DACXMZ010000210 DACXMZ010000000
VERSION     DACXMZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.211"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            175..828
                     /locus_tag="JD016_16140"
     CDS             175..828
                     /locus_tag="JD016_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HAU5001148.1"
                     /translation="MMKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFI
                     IEGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNV
                     FEEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKA
                     IRNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(968..2344)
                     /locus_tag="JD016_16145"
     CDS             complement(968..2344)
                     /locus_tag="JD016_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAU5001149.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2360..4273)
                     /locus_tag="JD016_16150"
     CDS             complement(2360..4273)
                     /locus_tag="JD016_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAU5001150.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4409..5041
                     /locus_tag="JD016_16155"
     CDS             4409..5041
                     /locus_tag="JD016_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAU5001151.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5180..5719
                     /locus_tag="JD016_16160"
     CDS             5180..5719
                     /locus_tag="JD016_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase"
                     /protein_id="HAU5001152.1"
                     /translation="MVFVEELWKKAIKFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(5895..6065)
                     /locus_tag="JD016_16165"
     CDS             complement(5895..6065)
                     /locus_tag="JD016_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAU5001153.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
BASE COUNT         2330 a          863 c          871 g         2212 t
ORIGIN      
        1 gaattatatt aaaataaatt atatatgaaa atatatatgt ataagtttat tctgcgtgag
       61 tagaatgaat ttgtcaagtt taatttagtt gtaattgtat cttttattaa aaataatata
      121 aattaaaaca tataaataaa atattttata caatatattg taaaggaggt aactttgatg
      181 aaagcagaaa aaacaagtat atatccaatt attaaaaata atttaaacaa aatatcaatt
      241 gttgaatata aaaagggtca aagatttgta gcatcagata aagacttaca agaagtgttc
      301 tttataatag agggagttgc attagtggag tgtactacta gaggaggaaa taaatttctt
      361 gttgacattg tacctgaaaa tgaatttata ggcaaaatca gttacatata tgaacataat
      421 ttgaaatgtg atatatttgc aaagactaat ataaaattat ttcgatttga aaaaaatgta
      481 tttgaagagt tttatctcaa accagatttt attgcactat ttcacagaaa atgtacaaga
      541 agaatatatg aattatataa aaccagaatg gtaagagaac tattttcttg cacagaagtt
      601 attgcctatt gtatatcaaa taaacaagaa aataatgtct gcaatataaa agctattcgt
      661 aatttcagtg aaatctattc aattagtaga aaaagtagat accattctct gctaaagtta
      721 actgaaaagg agatagtaaa aaaaatgggt aatacatatg aaattttaaa ctatgatgaa
      781 ttacataaat tggcatttga agtaaaagaa tttttggaag atgagtaaaa attgttactt
      841 aatcaaaata aagtataata atttctgtaa aaaatagaaa tagtctcact gaaaatcaaa
      901 aatactttag ttattttcaa tgagactgtt tagaaatatt gtatacttat aatgtatagt
      961 ataattatta atcaatttcc tttactatat taatagccca ttttttagat aatgctctag
     1021 gaacaccaaa tataaactta accacttctt cactataagt gagcaaaacg agtacaaata
     1081 taggtgcacc tttaaatgca gctataaaag ttagaggaat agaagctaac cacatacatc
     1141 ctaattctaa aaatagagca tatttagtat ctcctccact tcttaaaata ccaattataa
     1201 ccaatgtatt aaaagattta agagccatta gtattcccat tataaagaat atcttagtta
     1261 tatcagaagc taagctatca gaaacactaa acatttttaa taataatggt atatttaaaa
     1321 ttaaaataat tccaagtatt aaaccagctg aaaatacaag tatagaaaac tttttagcat
     1381 attcaatggc tcttttaata tggtcagctc caagttcatt tccaagcata atagatgcac
     1441 cagatgcaat acatacagct gtcattatga agaaattacc tgtactggtt gctatctggc
     1501 tagctgctat agcagaagta ccagctcttg catatgcaac agaatacagg acagttccaa
     1561 ttgcccatac agagtcattt acaaatatag gaaagctttt tgatgaaaaa gattttataa
     1621 aaattttatc aatagctttt agatttttta gaccaaactt taatatatag tcttttttat
     1681 aaaggtaaac ataagtaatc attaaaatta attcacatat tctagcaata acagtagcta
     1741 gagcagcacc ttttacaccc aatgcaggaa gaccgaagtt accaaatata aatccataat
     1801 ttaaaataac atttgttaca agtgcaaatg cactacaaat catacctaac ttaggatttc
     1861 taacacctct tgaaccagta ctaaatacag tacttacagc aattaatgga taactaaata
     1921 ctgcaatact aaagtaatct atacaaagtt ttaccacctc agaatcatac gaaaatatgt
     1981 gtataattgg ctttggtgtt agtaaagcag gaataacaaa tacaaaactt aaaacaattg
     2041 ccaatacaca agtaagacct gtgacttttc ttatattact cacatccttc tttccataaa
     2101 actgtgaaat aaaaactcca gcacctcctg tgataccaga taaggacata ttaaaaagaa
     2161 agaaaaattg gtttgcaact ccaactgaag ctacagatgt ttcccccaaa ctacttatca
     2221 taattgtatc tattacattt acagaagtag aaattaagtt ctgtatgata ataggtatac
     2281 ataaagatag aagtatttta taaaatcttt tattttcgtc ttttaaagaa aatagttctt
     2341 gcaaataatc ctctcctttt tacataaatt cttcccactc atcatataga gaggcaaggt
     2401 tattttctaa attaattttt tcttggctaa cttctttagc tttatctgga ttagagtata
     2461 cttcttcttg acataaaagt atatctaatt tttcaatttc aatctctaac ttttcaattt
     2521 ctttttctat attttgtctt tttattctat ttttcttttc tatttctctt tgttctctct
     2581 cttttctttt ttcatctttt atttgagttc tagttttttc ttctttttct tctataatac
     2641 tcatctcttg gatttgcctt tttttatcta tataataatc ataatttccc agatattcag
     2701 taattccatt ttcatctaaa actaaaattt tatcaacaac agtatttaga aagtatctat
     2761 catgtgatat tgtaaatata gtcccatcat aattagttaa tgcctcttca aggacttcct
     2821 tagaatcaat atctaggtga ttagtaggct catctaataa caataagttt gcatttgata
     2881 gtataagttt aagtatggca actctagctc tttctccacc actcaatgta gaaatcttct
     2941 taaaaacttc ttcatctaca aataaaaatg cccctaacat atttctcaaa gtagtttgag
     3001 taagatgagt attattttcc caaatctcat caattattgt gttatctaaa ttaagagttt
     3061 tttgttcctg atggaagtag gaaacatgta cattagttcc aaatttaata ttgccactaa
     3121 gaggttgtag ttcattagtt attattttaa ataatgtaga tttgccaaca ccatttgcac
     3181 ctataagtgc aactttttcc cctctatata tatctaaatc caagtcttta aaaagtattc
     3241 tttctccata acccatagat acatctctaa tttgaagaac atcattacca ctctggacag
     3301 aaggcgtaaa ttgtatacga gcttttttcc tataagcttc tggtttatct aaaacgtcta
     3361 ccttgtctaa tgctttttct ttacttcttg cacgctttag gtgtttttct ctaccataag
     3421 cttttagtct ttcaattgat tcttcttgct tttttatttc tttttgttgg tcttcaaatt
     3481 tcttaagctc tagttctttt tctattttag ataactcaac aaatttagag tagttaccat
     3541 tatatacttt aagttttttg ttgtgaactt caaaaattct atttacggat tggtctaaga
     3601 aatatctatc atgagatatt aacatgacag tccctttata ttgttttaaa aatacttcta
     3661 accattctat cgcttcagag tctaagtggt tagtaggttc atcaagtaac aatagagttg
     3721 gtttcttaag aagtaatttt cctaaaagaa ctctagtttt ttcaccacca gaaagtatgc
     3781 ttattggctt atccatatca ttgtcactaa atcctaatcc ctttagaact cctttggctt
     3841 cagatttata tccatatcca tttaaatctg aaaataattc tagtttatgt gaatattcat
     3901 ccatgatttt ttctataatt ggagagtttg tttttgtact ttcttcagat attttaacct
     3961 ccaattctct taaataggat tccatttcta tcaaattttt aaaaacttct agtacttctt
     4021 ccaaaatagt attatccgaa taaaaattgg tattttgttc taaatatccg atttcacaat
     4081 ctttagaagt gtaaatatct ccactatcat atccataaat tcctgaaatt attttaaata
     4141 aagtagtttt tccagtacca ttaacaccga ttataccaat tttatcgcct tcatttactg
     4201 taaaactaat attttctaat atagaatcta taccaaaact tttatttaag ttattacatg
     4261 acaaaacaat cataaatttc tacctccttg aactatctaa aattataatg ttttgatata
     4321 atataatcaa ggttatatgt attagttttg aaaaagaaat attttattgt gaatcaaaca
     4381 aaaaagagga cacttttagg aggtgaagat gttgggaaat aaaaatatat caatggcagt
     4441 tataagaagg ctcccaaaat atcatagata tcttggagac ttattagata gggatataca
     4501 aagaatatct tctaaagaat tgagtgatat aatagggttt accgcttctc aaataagaca
     4561 agatttaaac aactttggtg gatttggaca acaaggatat ggttataatg tagaagctct
     4621 tcatactgag ataggtaaaa ttcttgggtt ggatcgacca tacaacgcag ttcttgtagg
     4681 agcaggtaac ttaggacaag ctatagccaa ttatgcagga tttagaaaag ctggattcga
     4741 gataaaagct ttatttgatg caaatcctag aatgataggt ttaaagataa gagagtttga
     4801 agtattagat tcagatactt tagaagactt tataaaaaac aataatatag atattgctgt
     4861 attatgtata cctaaaaatg gagcacaaga agttattaat agagttgtaa aagctggaat
     4921 caaaggtgta tggaattttg cacctttaga tttagaagtt ccgaaaggtg ttatagttga
     4981 aaatgtaaac ttaacagaaa gtttatttac cttatcgtat ttaatgaaag aaggaaagta
     5041 gacaacttaa acatattaat aatgagcagt tgtgaagagt attgagaaag tcagaagatt
     5101 taatattaaa tcctctggct ttttgaaata ttaggataaa aatttaaata atgtatggaa
     5161 ataaacaata ttaaggagag tggtttttgt ggaggaatta tggaaaaagg caattaaatt
     5221 tcatggtcat gaatgtccag gattagctat aggatttaaa gcagcacttg cagcaagaga
     5281 atatttagga tgtaaacagt caggagatga ggaaattgta tgtatttctg aaaatgatgc
     5341 ttgtggaata gatggtatac aagtcgtgct aagttgcaca atgggtaaag gaaatttact
     5401 acttaggatg actggtaaat cagtatatca tttctttaat agggaaaatg gaaaatctat
     5461 aagaatatat gtaaagtcaa agccatttca aatggataga gaaggataca aaaagtactt
     5521 attagaaagt gattttgaag aaatatttga gattacagat actaagttaa gacttcctga
     5581 aaaagcaaga atatttaaat ctcttaaatg tgaagtgtgt ggtgaatttg catctgagca
     5641 atatataagg attgaagaag gaaagaaagt atgtcttgat tgctttaata aatatgaaag
     5701 attttatgag gagtattaaa attataaatt agattaaagt taaaagtatt agacatgtgc
     5761 taacagtatt ttaagatgaa ttaggtagca acataataaa gtatataaat aatattaatt
     5821 gaatcataca taattaaaaa gaggctgatt agtatttata aaataaaatc agtctcttta
     5881 tgaataacga attattattc tggttgaggt gcatctacag gacaagctcc tgcacaagaa
     5941 ccacagtcta tacaagaacc agcatctata acatatgcgt cgtctccagc tgatatacag
     6001 ctaacagggc attcagcctc acaagcacca cagcttatac aagcgtcagt aattttatat
     6061 gccattttga aatcctccct tttatatgta ttgtaattca ataacattat aacaaatatt
     6121 attgaatata acaattaaat taattctaca acaaaaactt tattatgata ttcttgtttt
     6181 aaagttttac aataaagttt aatctgctta tatttttatt tattttaaag aagtttattt
     6241 taaaaataaa attaaattta aaaagttatg tattga
//
LOCUS       DACXMZ010000211         6276 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.212, whole genome shotgun sequence.
ACCESSION   DACXMZ010000211 DACXMZ010000000
VERSION     DACXMZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.212"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            54..1340
                     /locus_tag="JD016_16170"
     CDS             54..1340
                     /locus_tag="JD016_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAU5001154.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1452..1925
                     /locus_tag="JD016_16175"
     CDS             1452..1925
                     /locus_tag="JD016_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1584..1586,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HAU5001155.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            1957..3267
                     /gene="grdB"
                     /locus_tag="JD016_16180"
     CDS             1957..3267
                     /gene="grdB"
                     /locus_tag="JD016_16180"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3004..3006,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HAU5001156.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3524..5056
                     /locus_tag="JD016_16185"
     CDS             3524..5056
                     /locus_tag="JD016_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HAU5001157.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5067..6212
                     /locus_tag="JD016_16190"
     CDS             5067..6212
                     /locus_tag="JD016_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HAU5001158.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
BASE COUNT         2397 a          764 c         1321 g         1794 t
ORIGIN      
        1 tttgagaaaa actcaaatgc atatacacaa attaaataac aggaggtgca agtatgcgtc
       61 ttgaattagg gaaaatcttt ataaaagata ttcaatttgg tgatgtaaca gaagtaaaag
      121 atggagtatt attcatcaat aagcaagaga tgctacaaga aattggtgga gatgagcata
      181 ttaaatctat agatatagaa ttagctcgtc caggtgaaag cgtaagaata acacctgtaa
      241 aagatgttat tgagccaaga gtaaaagttg aaggtaatgg gggaatcttc cctggtatca
      301 tgtctaaagt tgacactgta ggagaaggaa aaactcatgc tttaaaaggt gtagctgttg
      361 taactactgg aaaaatagta ggtttccaag aaggtataat agacatgact ggagaaggag
      421 ctaagtacac tccattttct aaattaaata atgtagtagt aatagctgaa cctattgatg
      481 gtttaaaaca atacgctcat gaaaaagctg ttagaatgat aggattcaaa gcagcaatgt
      541 acttaggaga agtagcaaga aatcttactc cagatgaagt ttcagtatat gaaactaaac
      601 ctcttcttga atcaataaaa gaatatccag agcttccaaa agtaggatat gtttacatgc
      661 ttcagactca aggtttatta catgatactt atgtatatgg agtagatgca aaacaaatag
      721 taccaacttt attatatcca acagaattaa tggacggagc aattgtaagt ggtaactgtg
      781 tttctgcatg tgacaaaaac ccaagttatg ttcatataaa caatggggtt gttgaagact
      841 tatatgcaag acatggtaaa gatataaact ttgtaggtgt aattataact aatgaaaatg
      901 tttatctagc agataaagaa agatcttcaa actggacttc taaattatgt aaatatttag
      961 gattagatgc agttatagtt tcacaagaag gttttggaaa tcctgatact gaccttataa
     1021 tgaactgtaa gaagatagaa atgcaaggtg ttaaaacagt tatagttaca gatgaatatg
     1081 ctggacgtga tggaggttct caatcacttg cagatgcaga tgtaagagct gatgctgttg
     1141 taacaggtgg aaatgccaac caagttgtag ttcttcctaa gttagataaa gttattggac
     1201 atttagaagt agtagatgta atagctggtg gttctgatgg aagtttaaga gctgatggaa
     1261 ctattgaagt tgaaatccaa gctataacag gtgcaactaa tgaaacagga tttggtcatt
     1321 taactgctaa aggatattaa gaagtatttt attaaacgat tgagtaatgt taataaaaca
     1381 tattatagat aattataata agtgttatag atatataaaa atatattaga aatataataa
     1441 ggaggattat tatgagttta cttagtaata aaaaggttct tataataggt gaccgtgatg
     1501 gtataccagg acctgcgata gaagaatgtg taaaaacagt agaaggagca gaggttgttt
     1561 tctcatctac agaatgcttt gtctgaacag ctgctggggc tatggactta gaaaatcaaa
     1621 acagagttaa agatgctgct gataaattcg gagctgaaaa tgttgtgatt ttactaggtg
     1681 ctgctgaagc cgaagctgca ggtcttgcag ccgaaacagt aactgctgga gatccaactt
     1741 tcgctggacc acttgctgga gttgccttag gattaagtgt ttaccacgtt gttgaggaac
     1801 caataaaatc attatttgat gaaagtgtat atgaagacca aataagtatg atggaaatgg
     1861 ttttagaagt tgaagaaata gaagaagaaa tgtctggtat aagagaagaa ttttgtaaat
     1921 tttaatgcct aaaagcaaaa ggggtgaaac taagagatgg gaaaactaaa agcagtacat
     1981 tatattaatc agttctttgc tggtataggt ggagaagaaa aagctgatac taagccacat
     2041 gtggcagaaa ctttacctcc aataagttta caacttgata aattattagg tgaagatatt
     2101 gaaatagttg gaacagtagt ttgtggagat agctatttca atgaaaatat agatagtgca
     2161 agtgaagaag ttttatcaat ggtaaaaggt tttgaaccac aacttttcat agctggtcct
     2221 gcatttaatg ctggtagata tggggttgct gctggaacaa taactaaagt tgtaaaagat
     2281 gctttgaata taccagcttt aacaggtatg tatgttgaaa atccaggagc agatatgttc
     2341 aaaaaggatg tttatgtagt agaaacttct gattctgcag caggtatgag aaaggcactt
     2401 cctaagatag ctaaacttgc agtaaaactt gctaatggag aagaaattgg aactcctaaa
     2461 gatgaaggat acatagctag aggaataaga gttaactact tccatgaaga cagaggttct
     2521 aagagagctg ttgacatgtt agttaagaaa ataaaaggtg aaccatttga aactgaatac
     2581 ccaatgccaa actttgatag agttgaccca agtaaagctg taaaagattt atcaaaatgt
     2641 aaaatagctt tagttacttc tggtggtata gttccaaaag gaaatccaga tagaatagag
     2701 tcttcttcag cttctaaata tggaacttat tctatagcag gagttatgga tttaacagaa
     2761 gagacttatg aaactgcaca tggtggatat gacccagtgt atgcaaattt agatgcagat
     2821 agagtattac ctgttgatgt tcttagagat ttagaaaaag aaggtgtaat tggaaaatta
     2881 catgagactt tctatacaac tgtaggtaat ggtacatctg ttgcaaactc taaaaaatat
     2941 gcttctgaaa ttggagcagc attagtggct gatggtgtag atgcagtaat attaacttct
     3001 acatgaggta cttgtactcg ttgcggtgca acgatggtaa aagaaataga aaaaactgga
     3061 cttccagtag ttcatatgtg tacagtagta cctatttcat taacagtagg agctaataga
     3121 atagttccaa ctatagcaat acctcatcca cttggaaatc cagcgcttga ccctacagaa
     3181 gaaaaagccc taagaagagg ccttgtagaa aaagcattaa atgctttaac tacagaagta
     3241 gatggtcaaa cagtatttga aaaataaaaa taacacacac atatattata tagataaaaa
     3301 tataaacaca cactaaaaat aaaaaataac tatacacaaa aaaaataata tagatttcat
     3361 gaatggataa attggagtta ttcattcatg aaatcttaaa aaatgccaaa atacataaac
     3421 aataatagaa tcaaatatac aataaaatac aatgattaga gcttttaata agtttaataa
     3481 gattaaaaaa taagtgttca taaaatttat ggaggtgcac aaaatgactt atccagtttt
     3541 aaaaggggca ggctatgtac ttattcatac tccagatatg atagtgcaaa atggaagtac
     3601 ttgtacagtt gaaagagcaa caaatccaga ttcagaattt ttaaaagaag taagtaatca
     3661 tataagaagt tatgaagatg tagttaacta catgccaaat caagtttata taggaaatag
     3721 aagaccagaa gaattaagag atttaccaat gccatggtgt gaacaaaaga ttgagggaac
     3781 tagaaatggt aagtttggag aaataatgcc tcaagatgag ttcatagctt taatgcaaat
     3841 aagtgatgca tttgatttag taaaattatc acaagaattt attgatgaag taaagccaaa
     3901 aatcgaaaat aactaccctg aaattgcacc atttgtaggt aaattaaagg gtgatgatat
     3961 agaagaaggt aaagaattag tagcaacaca catagctgaa ggattatacc atgatggaaa
     4021 atttgttggt tatgtaaaaa gagcacatga tgtagatgtt aacttaaatg cccatacaat
     4081 gtttgaaaat ctagtagtaa aagcatctgg agttttatct gctattcaaa tgctaagaca
     4141 tagcaaaata gaccctgctg aaatagatta tgttattgag tgttcagaag aagcttgtgg
     4201 agatataaac caaagaggtg gaggaaactt tgctaaatca atagctgaga tagcaggact
     4261 tcaaaatgca actggttctg acacaagagg attttgtgca gcaccaactc atgcattaat
     4321 tcaagcagca gctttagtaa aagctggcat acataaaaat gtaatggtag tagcaggtgg
     4381 agcaagtgct aaacttggaa tgaatgctaa agaccatgtt aaaaaaggtc ttcctgtact
     4441 tgaagatgtt gttggaggat ttgcagtact tgtatctgaa aatgatggtg taaatccagt
     4501 tataagaact gatttaacag gtaagcatac tgttggaact ggttcttcac cacaagcagt
     4561 tatgacagca cttataactt caggtcttga tagagctaat ttaaaaataa cagatgttga
     4621 tgtgtattca gttgaaatgc aaaacccaga tataactaag ccagcaggag caggagatgt
     4681 tccagaagca aactacaaaa tgataggggc acttgctgtt aaacgtggag acttagaaaa
     4741 gaaagaattg aaagattttg tttctaacaa aggattacca ggatgggcac caactcaagg
     4801 acatatacca tcaggagctc cttatatagg atttttaata gatgatttaa ctacaggaaa
     4861 tagaaataga gctatgatag ttggaaaagg tagtttattc ttaggaagaa tgactaactt
     4921 atttgatgga gtatctttta tagctgaaag aaatacaggt gttactgaag agacttcagg
     4981 aatatctaag gatgaaatta agaagataat agctgaatct atgaaaaaac tagcattaga
     5041 tatgttagaa gaataggggt gaggatatgt caaaaaaagt aatcgcagat gtattcttag
     5101 aagtagctaa tgcaatagaa agcggcgaat ttggcaaaaa agtaaaaatt ggtgttacta
     5161 cacttggaag tgaacatggt gtagaaaaca tggttaatgg tgcacaatta gcaaaatcta
     5221 atttatttga tattgtgctt attggaccaa aagtggaaac tgaccttgaa gtagttgaag
     5281 ttaatgatga aaaagaaatg catgcaaaaa tggaagaatt attagattca ggatatattg
     5341 atgcttgtgt tactatgcat tataattttc caataggtgt atctacagta ggtagagtaa
     5401 taactccagc aaaaggaaaa gaaatgatac ttgcaacaac aacaggaaca agtgctacta
     5461 atagaataga ggcaatggta agaaatgcca tttatggtat tgctacagct aaatcaatgg
     5521 gtaacaaatg cccaaaagtt ggaatactta atgttgatgg agcaagacaa gttgagaagt
     5581 gcttaaaaga attaaaagat aatggatatg acatggagtt tgcggattca attagagcag
     5641 atggtggatg tgtaatgaga ggtaatgatt tacttgtagg tgcacctgat gttatggtta
     5701 ctgatacatt atctggaaat atatttatga aagtattttc ttcatatact acaggtggag
     5761 attatgaagc tcaaggcttt ggatacggac ctggagttgg agaagactat gatagaaagg
     5821 tacttatagt atcaagagca tcaggttctc cggtagtagc aaatgcttta aaatacgctt
     5881 atgatgttgt aaagggagat ataagtaatg ttgcaagaaa tgaatttgca aaggttaaaa
     5941 aagcaaaatt tgatgacatt atatcctctc ttactaaaaa agaagttaaa gctgaaaaag
     6001 tagaagttaa aatgccagat aaggaaattg ttactcgtca aatagctggt gttgatataa
     6061 tggatttaga agatgcagta tctgagttat ggaaaaacgg aatctatgct gaaagtggta
     6121 tgggttgtac aggtccaata gttttagtaa atgaagctaa aggagactta gcagttgaaa
     6181 ctttagttaa agctggatat actgcaaaat aagtacattt tttatatgta aataagaaca
     6241 tatagttata attttattga ttataattat atgttc
//
LOCUS       DACXMZ010000212         6271 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.213, whole genome shotgun sequence.
ACCESSION   DACXMZ010000212 DACXMZ010000000
VERSION     DACXMZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.213"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            105..986
                     /locus_tag="JD016_16195"
     CDS             105..986
                     /locus_tag="JD016_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAU5001159.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEADQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRISNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1368..2171
                     /locus_tag="JD016_16200"
     CDS             1368..2171
                     /locus_tag="JD016_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001160.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            2183..3313
                     /locus_tag="JD016_16205"
     CDS             2183..3313
                     /locus_tag="JD016_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAU5001161.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNENHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            3317..4726
                     /locus_tag="JD016_16210"
     CDS             3317..4726
                     /locus_tag="JD016_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001162.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            4949..5446
                     /locus_tag="JD016_16215"
     CDS             4949..5446
                     /locus_tag="JD016_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HAU5001163.1"
                     /translation="MFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPIYSNN
                     TVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRGGKCE
                     ELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVSSYIS
                     SHIPQ"
BASE COUNT         2562 a          550 c         1102 g         2057 t
ORIGIN      
        1 ggtataaaga ttaaaataca taatcaaata tgtataagag gttttaaata gtattgttaa
       61 tgaacttgat tattgtttat aattacaaat tggaggagat aaacatgaaa attactgaag
      121 gatatatgcc ttttaaaggt tttaaaacgt attaccgtat agtaggagaa aatacggaag
      181 ggaagaagcc attagtatta cttcatggag gtccaggttc tacacacaac tattttgagg
      241 tattggataa gatagctgaa agtggtagac aagtaataat gtatgaccaa attggttgtg
      301 gaaattcatt tgtagaaggg catcctgagc tatttaatgc agatacttgg atagaggagc
      361 ttattgaact aagaaagcat ctaggacttg atgaaattca tctgttaggt caatcttggg
      421 gaggtatgca ggctatttgg tatgctattg aatataaacc aaaaggtatt aagtcttaca
      481 tcctttcatc tactctttct tctgcaaaac tttgggagaa agaacaaaaa agaagaatat
      541 catatatgag tgaggctgac caaaaagcat tacttgatgc agtaaatact ggagattact
      601 ctagcaaaga atataatgat gcattagaaa gatttatgga aatgtattgt gcaggtgaag
      661 tgacagaaga ttctccagag tgcctgagaa gacctaaaaa atcaggttct gaagcatata
      721 ttgtaggatg gggacaaaat gaattttctc ctacaggaac tctttctgga tatgaattta
      781 cagatagatt acatgaaata aaagagcctt gtctagtaac tagtggagca atagatttat
      841 gttcaccata tattgcaaag actatgtatg atagaatatc taatagtaag tgggaattat
      901 ttgagtattc aagacatatg ccattcgttg aagaaaatga gaaatatata aaagtcttaa
      961 ctgagtggtt gaatgcgaac gattaaaaac tatataaatt tatataaaaa aatgagtgtc
     1021 tcaaaatgaa ctttttagtt cattaggcac tctttttgca tgaaatcaaa tatattttca
     1081 ttatttgaac aataaaatga ggcttatatc tattttggga aagtaatttt ttattactag
     1141 cgttagattc taatctaatt gtatacttat tgaaataata gaaatatatt ggtgtttata
     1201 acttaatttt attgtttttt agaaattttt ttatatattg gtataattat tctgtttaaa
     1261 tttgattaaa aagtatatta attataataa gaaacaatta tataaatagg aatagattat
     1321 tataattaat atagatataa aaataattat agaaggaaag gcacattatg gaaaaattga
     1381 gagttattga aatgataaaa aataatccca atattatagc tacaatagat aatccaactg
     1441 atgaaatgaa attgttagct attaaagaaa atgggcttgt actagaatat ataaataatc
     1501 caaccagaga gatgcaagaa ctagcaatag ataataatat tagagcaatt aaatttatag
     1561 ataatccaac agaagatatg atgataaaag cagtaaatga gggatggagt atactggatt
     1621 atatcaaaaa cccaactgat aaagttattg aaatggctat aaatcaagca ggatgggcaa
     1681 ttaaatatgc taaaaatcca agtgaagaat tacaactgtt agcagtaaga aaaaattatg
     1741 attcgataaa gtttataaaa caaccttatg aaagtgtaca agaggaggca gtgagaataa
     1801 gttatgatgc cttgagatat atagattcac ctagttataa tgcagaactt atagctataa
     1861 aaaataatga agcagctatt agctttataa ctgatttaga taaaaataaa atgttggaat
     1921 ttttaaaagt gaatatttta gtgattaagt acattctgaa aaaagtatct aaagatgaat
     1981 tagagagtgt attaaaagaa gtattatcca aagaagatgt tgaggaaaag tatattagag
     2041 attttttgaa ttgtagtgtt atagatagaa atagtgaaag tataccaata gataaaatta
     2101 tgtttattta taaatatgga agtaagacag caaaaaaaat agctgtagat gagaaattaa
     2161 agattatgta ggagagagaa aaatgaattt tatagacaca ttaaaaagcg ttgacttaat
     2221 attatccact ggagaagtac ctttaatagt aggtgaaagt ggaataggaa aaacagcttt
     2281 agcaaataag cttgccaaag aaaatgattg gagtttaatt gttatagatg gaaacttact
     2341 taaagagggt gaaataggtg gtcttccaac tatagaatct tatgtaggag ttaattctaa
     2401 tggatataag actgaaaaga aaacaacagt gtatgctgtt cataacaaat taagagaaat
     2461 tgatgaagag atatctaaaa gtaagacagt tcttttattt atagatgaga taaatcgttg
     2521 tgagcataca gtacagcaag aacttatgaa tttaatttta aatagagaaa ttaatggata
     2581 taaactgcat gatgatgtaa agattttagc agcaatgaat ccatcaagta aatatggttc
     2641 agattttgat tatcaagtgg tagatatgga tgcagcccaa gaaaataggt ttgtttggtt
     2701 aaatatggag agtgaccata cacaatggat aaaatgggca atagatgagg gtattgaaag
     2761 aaaagttatt gagtttattt ccacttttcc agaatatctg cataaaataa atgaagatga
     2821 tgtaagagct actcctagaa gttatgagag agtatctaaa atttataaag tttataaaga
     2881 aaaaaataat tctattccta gagcagtatt tttaaatgtt gtaaagggta atgtaggtaa
     2941 agttattgca gaagaattta taagttttat tgaatcaaac tctgaaccat taatatccta
     3001 tgaagatgtt tttttaggag aatctattga tgagtctatt gtagaaagag taaaaaatga
     3061 gaatcataca agactttatc tatcagctat gaacatttta aaagacttag aattaaatat
     3121 taagaatgat aaatatgaat caaaccacta tattaataga tttattgaat ttttaaaaat
     3181 gtatcctgta gatttaatga taggtattat gaaggatatt agaaacagtt atattgaagt
     3241 atataaaaaa gctatagaaa atgaagagtt tgtaaaatca tattttgaat cttatagttt
     3301 aataagggga taatgtatgg aaaattattt tgataaacaa gcaaaagagc tttataataa
     3361 agcaagtgaa attataaata ctcattctat gctaaaggca aatcgtgcta atgagaaatt
     3421 tgatatagat ataccacaag attttaagta tgagtttttt agtcttgtag ataaagtgaa
     3481 tttaagtctt atggaagaag aagataattt ttatggatat tttttgtttc agatgtcaag
     3541 agaaataaga tttgatataa gtagtcctac aggtgtgaat tttaagggag ctaaatatgt
     3601 tatatatttt aatccaataa tctttttaac gcttgacata aagcaaatgg agactacaat
     3661 taaacatgaa attcatcata tattatctat gcatttgatg agagcaaagg agttaaaagg
     3721 aaagtatagt acattagcaa ttaatatggc aatggatata gtagtaaata aatttctaaa
     3781 caatttacca ccatatgcaa ctaccttaga gtgggtaaat ttaaagtatt ccttaaaact
     3841 tgaaccatat gaaccttttg aatattatgt agaaaagatt cagactgaat tagatttgat
     3901 ggaagtggat gaagatggtg aggaagatga tagtgataaa tatggagata tagaaattga
     3961 ttatagcccc gaaagaactc atgatatatg ggatgaatcc aatgagatag atgaaaatac
     4021 ccttagagaa tttactgaaa aatttattaa cagttctcaa aaaggtaaag ttcctgctta
     4081 tttagaaagt atgatatcat cacttaaaaa tagtaagggt gaattgccat ggaatttata
     4141 tcttaataga ataatgggaa cagttgaaaa taataaaaag aagactataa caagaagaaa
     4201 tagaagacaa ccaaatcgtt tagatttgag gggagaactt agaagtcata agtcagaaat
     4261 agcagttgca cttgatgtaa gtggaagtat tagtgatgaa gagtttaaac aagctattaa
     4321 agaagttctt agtatagtga aaaactataa tcatgaaatt acaattatag aatgtgataa
     4381 tgaaattaga cgtgtatata aagtgagaac tgaaaaagat ataaaagata gaattaggat
     4441 aggtggaggc actagatttt ctccagtctt tgagtatgct aataataaaa agattaattt
     4501 gttagtctac tttactgatg gtaaaggtga aaataaacta caagtaatac ctagagggta
     4561 taaaatttta tgggttattt ctggaagagg agataaaatt tcattaaaag aaccttatgg
     4621 agcagttaaa aaacttacta aggttaaaac aaatgatgaa atattagaaa taaatgatgt
     4681 aagaaatgat gggtactcaa tgaatagcca agcaccaatt ttataggtat tcacaggaga
     4741 taagctttat taatttattt tgatatgctt gttataaaaa tctaaaaaat acattaatca
     4801 atggttaatg tattttttaa ttttaataat ataagtttta cttacattta agtcttaaaa
     4861 tgtgttaaaa tataatatat agcagatagt tttggcaagg tggatttagt tgtcaaaact
     4921 atttttttta ttaaggatgg tgatgttttt gtttaagatt gatgattata taatgtatgg
     4981 tatgacagga gtctgcaaag tcatggatat aacaaatgaa cgatttacaa atggaataaa
     5041 aaaagagtat tatgtattga gtcctattta ttctaataat acagtaataa aaattcctgt
     5101 agataatgaa aaagtaccaa tgagaaagtt actttctaaa attaatgtgc tttcattaat
     5161 aaatgatatt cctaatatgg atacatcatg gatagataat gaaaaattga gaagtgagca
     5221 atttaaaaaa atactcagag gtggtaaatg tgaagagtta ataaaattag taagaagtat
     5281 agataataac agagagtatg taaaatctat tggtaaaaaa acacatcaag cagatgataa
     5341 tataatgaaa gaagctgaaa ggttattaaa tgaagaattt gcaactattt tagatatttc
     5401 tcctaatgaa gttagttcat atatttcaag tcatatacct caataaagtg ttttagtatc
     5461 ctttatttaa tctattttaa gtattattta tgtatgctgg ttttggtaaa ataaaatata
     5521 taattaaatt atataaaaaa taataatgat tgtattgaag aattacagaa tagtgcggag
     5581 agcctactcc tttaggtgtg agatggatat cacttaaaga tttaaattgt tattgtagat
     5641 gtttttaata taaaacaaga ttaataagtg tattgtaagc tttaagtgaa attgatacta
     5701 taatgagtta cttagatttt aaaatatata atattataaa aaatcctttt aggttttgaa
     5761 tgaataagat ggatactatg ttgaatagaa tccatcttat ttaagtttta tttatgttgt
     5821 ttatttgtaa gcattatttt tgtacatgaa aacacagtgt aaaaatatgc ttagaattat
     5881 gaagttttat taaaaagata ttcgacaaaa taagactaaa aatattctta aacttataac
     5941 taagataaat attgtatatt tatagtgtag cctgaaaaat tgtgaatgta cgaaatttat
     6001 ttagtatttt attgaaacaa agtagaagaa gtactatagt ttatctttga atagattgtt
     6061 attttttagt gaaaaagtta ttaggttaat acataaatat aatagcttat aaatagttta
     6121 agcatgttaa aaaagtagtt attagagata tatagttaca ttgcttataa gatacttaaa
     6181 atgatagcat tcacttatat ttagaaaatt aagataaaaa atcattttta tatagtaaag
     6241 catacctaaa tttagaaaat aattgaattt a
//
LOCUS       DACXMZ010000213         6195 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.214, whole genome shotgun sequence.
ACCESSION   DACXMZ010000213 DACXMZ010000000
VERSION     DACXMZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.214"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(41..2062)
                     /locus_tag="JD016_16220"
     CDS             complement(41..2062)
                     /locus_tag="JD016_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAU5001164.1"
                     /translation="MMTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAML
                     TLLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDL
                     KRAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKE
                     LISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDD
                     FYISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVA
                     TKEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKK
                     VKSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHL
                     RDGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLL
                     AGYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFIL
                     PYDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINA
                     IYKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDE
                     GRSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKISIKEAA"
     gene            complement(2330..3106)
                     /locus_tag="JD016_16225"
     CDS             complement(2330..3106)
                     /locus_tag="JD016_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAU5001165.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     NTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(3163..4134)
                     /gene="asrC"
                     /locus_tag="JD016_16230"
     CDS             complement(3163..4134)
                     /gene="asrC"
                     /locus_tag="JD016_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HAU5001166.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(4147..4938)
                     /gene="asrB"
                     /locus_tag="JD016_16235"
     CDS             complement(4147..4938)
                     /gene="asrB"
                     /locus_tag="JD016_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HAU5001167.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(4939..5946)
                     /gene="asrA"
                     /locus_tag="JD016_16240"
     CDS             complement(4939..5946)
                     /gene="asrA"
                     /locus_tag="JD016_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HAU5001168.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
BASE COUNT         2022 a         1132 c          547 g         2494 t
ORIGIN      
        1 tactttattt taaatatatg gctattctat aatttaatat ctaagctgct tcttttattg
       61 aaattttagt tatattactt attctataca tttctttgac accatttaga gtttgcagta
      121 agtttagaca tattatcttg tcacctagac ttttagtatt tacaatactc tctaaaatta
      181 cacttcttcc ttcatcatac ttatctacat tgtaatttga aatataatta gtatctctca
      241 atattttttc aacatcttca tcttttattt tatttttatt aacatttata tatagattat
      301 acatattact tattagtaat ttctgatttt ctaaaaaatc ctcttgatgt ttatctttat
      361 atatagcatt tatcaacata agtctatctt caataagtga tggtatcaat aataaatttt
      421 ttatatagta aacatcagct acattttcta tagacttatt gacttcatca attatatttt
      481 taactattcc tttatacatt tctataacat gttgatagcc tgttttagca tcatatggta
      541 atataaactt atttactatt agtgcaataa ttgctcctaa tgctacatat gaaatcctgc
      601 ttatggttaa aacatttggg tctcccataa ctactgctga accaagagct gaaacagtta
      661 cacataccat taattttcta taatcaacta cataagagtt tatataccct gctaaaagaa
      721 ctattaacga ccttaatgta ctatctttta ttattgaaaa taatacaata aatattacag
      781 ctcctataaa tgtaccttct attctttttc tagacctttg tatacaattt tctgcgtaag
      841 gttgtgttaa tgaaaagaca gttaacatta tccatcttcc atctcttaaa tggaaataat
      901 ccataataaa acctgctaca gctgtagcta aagcaatctt tactgcataa gaaaatctta
      961 tagaatttaa attaaagttt ttcttatata ttgatagtcg tttaaattca tgaggtatct
     1021 ttgattttct ttcagctttc ttttcgtctc ttgattttac ttttttataa tctactaaaa
     1081 actcatataa attttctaaa acactataaa tttcttttaa atttatattt ttagtatcct
     1141 gagcaataac ataatctcta aatcctaata tctctttatc agttatggta tctttatttt
     1201 ctaaacacat cttaatatag tttatttctt tcgttgctac aactaaaaca tctgatttac
     1261 tatttatgct actttcttct aaaatatttt ttatatcttc atcttcaaat tctttactat
     1321 cctttttgta tctttccagg attaaactta ttctttctaa tgaaaataaa atattcatta
     1381 tgtctatacc tctaatactg atataaaagt catcttttct attatcaaaa ataaccattt
     1441 tcaaattttt agatatagaa tttattctat aattacagct atctattttt ctcatactag
     1501 atttaattat agaactatca tcattaatag tattttttaa aagactaatt ttttctaata
     1561 attcatcaca tatagatatc agttctttat ctccgacatt tttcatccta tttctattca
     1621 taacaaattg agcaatcatt attataactg ctccaacagc taaagccgac aatctcatag
     1681 gaaattcact ttttcctaca ggtatactta ccataaatat atacattaga ccaaagggta
     1741 catatactgc tctttttaag tcataactaa atacataccc tattataaat aatgctataa
     1801 aatttaataa aactcctaaa tacatatttt gtactgcaaa aaatgatagt attcctaaga
     1861 ttatatttat tgctaaatac ttcagtaaat ttttaattgg agaaatagtt aaatctcttt
     1921 ccaagagtgt tagcatagct gttactatac ctacacctat aagtgtattt ttacttccaa
     1981 ataaatatat aaatatagaa ataaatgcta ttataaaaat aaacaacttt gtttttgata
     2041 ttactagttt ctttgtcatc atatcccccc taaaaatttc aacaagacca attttatcat
     2101 atttgttatc ttattcaaaa tgcgcttttt tacggtagga ctttaacttt ttttatattt
     2161 agaactatat aattcatata taaacgtatt ttttatatat ataactgata tattttcgta
     2221 tgctcccata ttttttttgt ttcaaatatt ttttcaaaaa tataaaaata actctctcaa
     2281 caatactaaa aatgtattta tcaagagagt tataatttat acaataaatt taattaagct
     2341 tttctttacc taatatataa gttccaagac ccataaataa agctcctcca acaatatttc
     2401 caagtgttac agctactaaa ttataaatag ctcctccaat agtaactgta tttattgttg
     2461 gtgaaaataa tgaaacacta taaattgtca tatttgctac actatgctca aagcctgatg
     2521 ttataaaagc aaataaacat aagaatatca taattatttt tgcagtatca ctattagttc
     2581 taaacgaaca caacactgat acacatacta gaatattaca taaaatccct ctaaagaaaa
     2641 gagctgtaaa tggcatggat gctttactag ctgctgtatt tgcaaaaaat tcagctacag
     2701 gtcctttgtc tactagacca gttcctacaa atataatgcc taaaaccaaa gctccgatta
     2761 aattacctac ccaactgtat gcccatattt tagaagtatc ttttatactt acacctttat
     2821 ttaacatacc agcagacata accatattgt ttccagtaaa taactctgtt cccgtcatta
     2881 taaccaaact tagtgctatt gcaaatgata accccattac tatcttagtc ataggtgaac
     2941 ctgcatcagt caaaagtcct ccaatagtaa atatcaaaag tatacctata cctacataaa
     3001 gtcctgcaaa cgcagaagat accaaatatt ttactttact tgtgtttaat aaatttatct
     3061 tatttatagc tgcattagtt aatttatcca aagtttcttt atgcataatt ccctcttcct
     3121 tctattaaat tttaatttat tcaaatatat gttttactca atttatagat atttaattcc
     3181 actccagtat atgttttcgt acattttagt tatttctgga agctctacac cttcaagtgc
     3241 ccatttttta aattccataa atcctgttct atcaattata tatcctatat gttcttttcc
     3301 tcctggtgca tctttatcta tatacttttc tacatatcta taagtattaa gtattatctt
     3361 tattatgctg tcctcatcta cccataaaag aaaatcttct gctagtctag ggtttttctt
     3421 tcctgttcta cccattatag ctaatctata gtattttttc tcatctctag tccatgcatt
     3481 tgttgggcaa tttagtacac actctccaca gcctatacac ttatctttat ctcttaccac
     3541 cttattattt tctgttttta atgctccaac agatatctta gaacattttt ttacacatgc
     3601 tccacaattt acacatctat ccttttcata tattggtaaa gccataccaa taattccaaa
     3661 gtcattcatt ctagctttta tacaatcatt tggacaacca gttaaagcca ctttaaaatg
     3721 taaatcattt ggaaatatag ctttttctat tcttttagca aattcagttg tattatattg
     3781 agcctttgga cagactttat ttcctataca agctgctata tttcttgtac ctgccgctgg
     3841 ataacctgcg tttttctcac tttgatttat attcattttt tcaataacag gttgtataat
     3901 tttatttatt tcttccatat cttccatatt tattcctaat atttcaaacc cctgtcttgt
     3961 agttatgtgc acctgtccat tgccgtactc acttgcaatt ttagaaacta cagctaaact
     4021 ctcagggtca ataagacctc caggtactct aactcttaaa gaagtctcat attttgtttt
     4081 tgtaattcta taagcatttt tcattacttt tttggtattt aaatctctta tcacaaactt
     4141 caccccctaa tcaataagat ttttagcata tgaataatca aatactggtc catctaaaca
     4201 tatgtaagta tcatccattt tacaatgtcc acatttacct attccacaac acatttttct
     4261 ttcataagaa acccaaatgt tcttttcatc taaatctctt tttaaaaatt cttctactgt
     4321 aaatttcatc atcattggag gtccaactac aactatagaa gtattgttta tatctttcat
     4381 atccaattct ggtatgtatt ttgttactaa tcctacatta ccactatatc cttcctctgc
     4441 tccatctaca gtaactaaaa tatcaagttt tttactccat ctctttaaat catctgcaaa
     4501 taaaatatct tttggtgatt taaatcctac tattaattta aacttttcgc atttttcttt
     4561 attattataa aaatattcaa ctattcctct tacaggggct aatgcacttc ctcctgccac
     4621 tactacaatt tctcttcctt cataaaggga tacatcaaat ccatttccat aaggacctct
     4681 tataaaaaaa gaatctcctt ctttataatt aaacaactcg ctagttactt ttcctacatt
     4741 tcttattgta aagtcaatat aatcctctcc atatcctgat actgatattg gactttcacc
     4801 gtatttaggt atagatatct cataaaactt tccaggcaat acaccttttg tattgcagtt
     4861 tactctaaaa gtccattctt tatcagtatg tttaactata tctagtattt ttgcacttac
     4921 tggtatatat gggttcattt atacttcctc cttactaatt actaaatcat ttattttttc
     4981 tatacacttt gaaaatgata tgtattgcgg acaggcatca tcacatcttc cacaaccaac
     5041 acacatatga tatccgaatc tttttttaaa atcatgtatt ttatgcatag ttttaaatct
     5101 cattctttca ccttgttttt ttctaaaaga atttccacct gctatatctg tatatccatc
     5161 tacttgacaa gaagcccaaa cccttcttct ctcacctaca ttttcgtttt ccttatagaa
     5221 aatatcctgc attgtaaaac atgaacatgt tggacataca aaattacatc taccacatcc
     5281 tatacatctt gtatcatatt catcccatat tgaatttcct atcatttcat ttgaatcaat
     5341 attctctggc acatctacag atattaaatt atcagttaca taatcaacat taaattcagt
     5401 tgcttctcca ttaaatactt caaattcact atcctttata tctaagtaca gtgtatctcc
     5461 ttctatattt aatccaattg aataattatc agttttgtta gaatccatgc tcacacaaaa
     5521 acagtttcta aaactttcct tacaccctac aagtgcaaac tttactttat ctctgatatt
     5581 tttataaaaa taatcttttt cttcaccatt atttagatat atttcatcaa gtctttttat
     5641 accatttatg tcacaagctc ttaagaaaat taaaattttc ttgttgtcta aatcgctttc
     5701 cttaaactct ttttcagtaa aataaaacaa tacttgattt atgggaagta caacttcttt
     5761 tggtgaaaag tttgctttct tagcaaattc catttcttca aaagcgttta cctccttgta
     5821 tcttataaca tcagtatctg aaaaagtacc tttaaatggg attaaaactg gtgcaaatat
     5881 cttgtagtct ttttttagat aattcagacc ttcattgaac ttagatgaat ctaattttag
     5941 tttcattttt atcacctttc ttatatttta gttttactta cttgctatct tgtttaaatc
     6001 ttaacacttt attttctatg cttccgtgat aattatcaca gtttcaaaaa taataaaaaa
     6061 aatattaatg gagtttatta agttctgtta atattttttt attatctaca ttattcgatt
     6121 tcaaatctaa tcacttaaat acaatcatta agtacaataa aactactatc tactcaattt
     6181 tttgaataaa aaaga
//
LOCUS       DACXMZ010000214         6190 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.215, whole genome shotgun sequence.
ACCESSION   DACXMZ010000214 DACXMZ010000000
VERSION     DACXMZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.215"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            136..1158
                     /locus_tag="JD016_16245"
     CDS             136..1158
                     /locus_tag="JD016_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAU5001169.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            1218..2225
                     /gene="gap"
                     /locus_tag="JD016_16250"
     CDS             1218..2225
                     /gene="gap"
                     /locus_tag="JD016_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAU5001170.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            2447..3649
                     /locus_tag="JD016_16255"
     CDS             2447..3649
                     /locus_tag="JD016_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAU5001171.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            3675..4418
                     /locus_tag="JD016_16260"
     CDS             3675..4418
                     /locus_tag="JD016_16260"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAU5001172.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            4427..5959
                     /locus_tag="JD016_16265"
     CDS             4427..5959
                     /locus_tag="JD016_16265"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAU5001173.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLREDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         2478 a          727 c         1241 g         1744 t
ORIGIN      
        1 ataaaaaaat aaaaaagttt ttgaaaaact attgaaatct atacgcaaat cctataaaat
       61 aaaaatatct tgtgggactt aaataaacac tagggacaaa aaaagtcccg aaaggaaagc
      121 aataaggagt atgttatgaa gaacttatta gaaattcaaa agaaattgat tccacaggca
      181 attgaactta tggaaagaag atactctatt ttaagacaaa tatctttaag tgaaccaata
      241 ggtaggagaa cattatcaaa tgtattagag attagtgaaa gaataatcag gtcagaaact
      301 gagtttttaa aagaacaagg acttatagac gtagctgtat ctgggatgac tatcactaaa
      361 gaaggttctg atttgttaga taaacttaag gacatcatga gtgacatcat gggattgtct
      421 aagttacaag atagagtcag agaaaagctt ggaataaaaa gggtaatatt agttcctgga
      481 agctgtgatg aaaatggaag tttactaaaa gatgtaacta gatatggatg cgaatatttc
      541 ttaggcattt taaaagatgg agatatcgtt tcaataacag gaggaagcac tatgttggag
      601 ttttcaaacg ttattaaaac tgataagaga tatccaaact ctacaatagt tccagctaga
      661 ggaagtatgg gaactgatgt agagatgcaa tctaacaata tagttgcttc aataagtaag
      721 aaattacatt ctaattgcaa acttttacat ataccagatg agttggatga aagtgcaatg
      781 aaaactctta gccaagtgcc agagattaag acaactttgg agtatattaa aagaacagac
      841 attttagttt ttagtatagg tagagcagat gtcatggcaa agagaagaaa acttccagaa
      901 gacaaggttg atgagatatt atctaaaaat gcagttggag aagcatttgg atattatttc
      961 aacaaagatg gagaaatagt ttacaaactg aacactgttg gaattgattt agaaacagtt
     1021 aaacaagtaa aagaaactat tgcaatcttt gcagggactg aaaaagtaga agccttatta
     1081 gctatatcaa atatgcataa aaacatggtt cttgtaacag atgaagaaag tgcttataag
     1141 atattgtcat tattataaaa gtcaagacct agcacagagc tagaaaacat acatttactt
     1201 tataggaggt agcgaaaatg gttaaagtag ctataaatgg atttggaaga ataggaagat
     1261 tagcattaag aaaaatgatg gagcaacaag ataagttcga agttgttgca ataaacgact
     1321 taacagacgc taaaatgtta gctcatttat ttaaatatga tacagctcaa ggaagattta
     1381 acggagaaat agaagttaaa gaaggtgctt ttgtagtaaa cggaaaagaa ataaaagtaa
     1441 cagcagaaag aaatcctgct gacctaccat gggctgaatt aggagtagat atagtattag
     1501 aatgtacagg attctttaca tctaaagata aagctgaagc tcatatacaa gcaggggcta
     1561 agaaagttgt tatatctgca ccagctactg gagatttaaa aacaatagta ttcaacacaa
     1621 acagtgacat attagatggt tctgaaacag ttatatcagg agcttcttgt actactaact
     1681 gtttagctcc aatggctaaa gtattaaatg ataaatacgg tatagaaaaa ggattaatga
     1741 ctactataca tgcatacact aatgaccaaa atactttaga tggtcctcat ccaaaaggtg
     1801 atttaagaag agctagagct gctgctggaa acatagttcc aaacactaca ggagctgcaa
     1861 aagcaatagg cttagtaata ccttcattaa aaggaaaatt agatggggca gcacaaagag
     1921 taccagttgt aactggttca ataactgaat tagtttgtac attaggaaaa aatgttacag
     1981 ttgaagaaat aaatgcagct atgaaagaag cttcaaacga atctttcgga tatactgaag
     2041 aaatgttagt atcttctgac ataatcggaa taagctacgg ttcattattt gacgctactc
     2101 aaacaaaagt tatggaagta gatggaaaac aattagttaa agttgtttct tggtatgata
     2161 atgaaatgtc ttatacttct caattaataa gaactttagg atactttgca caattagcaa
     2221 aatagtcagt tttgagaaaa tagaaaagta gattgacaaa gtccgagttt gtagtttaat
     2281 actacggact caggactttt tttaagaaaa aatatataaa atacacctaa ataatgttac
     2341 atactatata taaaaatatg ataatatgat atatttaata gggtaaaagt atgtactata
     2401 ttatacttgg caataaaaaa ttactttatt aaaaggagag atatttatgt caatgcttaa
     2461 caaaaaaact atagaagata ttgatgtgtg cggaaaaaaa gtattagtaa gatgtgactt
     2521 taatgtgcca ttacaagatg gtgtaattac tgatgaaaat agattgaacg gagctttacc
     2581 aactattcaa tatttaattt ctaaaggggc aaaagttata ttatgctctc acttaggaaa
     2641 gccaaaagga gaagctaaac cagaattatc tttagcacca gtagcaaaga gattatctga
     2701 gatgttagga aaagaagttg tatttgcagc agacgataat gttgtaggag aaaatgcaaa
     2761 gaaagcaact gaaaaaatgg aaaacggaga tgtagtatta ttagaaaata ctagatatag
     2821 aaaagaagaa actaaaaacg aagagaattt ctctaaagaa ctagcatcac ttgctgaaat
     2881 atttgtaaat gatgcatttg gaactgctca tagagcacat tgctcaacag ttggtgcagg
     2941 cgagttttta caagagagag tttgtggata cttaattcaa aaagaattaa aattcttagg
     3001 agaagctgtt gcaaaccctg taagaccatt tacagctata cttggaggag ctaaagtttc
     3061 tgataaatta gctgttataa acgaattatt agaaaaagtt gataatctaa taataggtgg
     3121 aggaatggct tatacattct taaaagctca gggttatgaa gtaggaactt ctttacttga
     3181 aatagataaa gttgaatatg ctaaagaaat gatggaaaaa gctaagaata agggtgttaa
     3241 cttattatta ccagtagacg ttgttatggc agaccacttt gcaccagatg ctactccaat
     3301 agtaacagaa gatgctaatg taaaagaaga ttacatggga ctagatatgg gacctaagac
     3361 tatagcaaac tttgtaaaaa ctataaaaga atcaaaaact gtagtatgga atgggccaat
     3421 gggagtattt gaatttgaaa actttgcaaa tggtacatta tcagtagcta gagctatggc
     3481 agagttgact gatgcaacta ctgtaattgg cggtggagat agtgcagcag cagttaatca
     3541 attaggattt ggtgacaaga tgactcacgt atcaacaggt ggaggagcat cactagaatt
     3601 tttagaaggt aaagaattac caggtattgc agcattagat aataaataaa ctaatatata
     3661 gaggagattg taaaatgaga aaacctataa ttgcaggaaa ctggaaaatg cataaaacaa
     3721 taaaagaagc tttagaattt gtaaatgaag ttaaagataa agtaaattct gataaagtag
     3781 aagctgttat atgtgcacca tttactttat taaaggattt aaaagaagct actaagggta
     3841 caaatataaa gataggtgct caaaatatgc actttgaaga aaaaggagca tttacaggag
     3901 aagtttcacc tcttatgtta aaagaaatag atatggacta tgttgtaata ggacattcag
     3961 aaagaagaca atacttcaat gaaactgatg aaactgtaaa caagaaagtt ttaaaagcat
     4021 tagaagtagg aatagaccca atattatgtg ttggagaaac tttagaacaa agagaagctg
     4081 gaaaaactaa agatgtttgt aaagttcaag ttgaaaaagc tttagaaaat gttcttaaag
     4141 atgatttagc aaaagtagtt gttgcatatg agccaatctg ggctattgga actggtaaaa
     4201 ctgcaactgc tgaagatgct aatgacgtta tatcttatat aagagaagtt ataaaaggat
     4261 tatatggaga attagctaat gaagttagaa tacaatacgg tggaagtgtt aaaccttcaa
     4321 atgtagccga aataatgggt caaagtgata tagatggagc tttagtaggt ggagctagtt
     4381 tagctagtaa tgattaccta gaccttgtta atttctaagg agtgaaatga tgaaaaaacc
     4441 agttgcttta attattatgg acgggtttgg atacaataag gatgttaagg gaaatgcaat
     4501 tgctgagtcc aaaactccta atttagatag aataaaaaaa gagtatccaa acacattgat
     4561 aaatgctagt ggtcttgatg ttggtctacc tgatggtcaa atgggaaatt cagaagttgg
     4621 acacactaac ataggtgcag gtagaatagt atatcaagat ttaactagaa taacaaaatc
     4681 tataaaagat ggtgactttt ttactaataa agttttatgt gaagcaatgg ataatgcaaa
     4741 agaaaactct cttcatgtaa tgggactttt gtctgatgga ggagtacatt cacatataga
     4801 ccatcttaag gctataatca aaatggcaaa agataaaggc gtacaaaagg tgtatgttca
     4861 cgcatttact gatggtagag atactgaccc tcaaagtgca ttagaatatg ccaaagaagt
     4921 acaagctagt atggatgaaa ttggagtagg tgaatttgct acagtttctg gtagatacta
     4981 cgctatggat agagataaga gatgggaaag agtagaactt gcatacaatg ccatggttag
     5041 aggtattggt gaaaaagcaa attctattga ggaagctatt caaaactctt atgatgatgg
     5101 aaaaaatgat gagtttataa tgccaacagt tattatgaaa gatgataaac cagttggaag
     5161 tataaaggaa aatgattcaa ttatattctt caactttaga ccagatagag ctagacaaat
     5221 aactagagct ttagtttgtg aagagtttga tggatttaaa agagaagata ttaaaaactt
     5281 ctttgtatgt ttaacagagt acgatataac tatagagaat gtgcatatag catttggacc
     5341 tcaatcctta gccaacactc taggtgagta tctagctaaa aatggaaaaa ctcaacttag
     5401 agctgctgaa actgaaaagt atgcacatgt aactttcttc ttcaatggtg gagtagaaga
     5461 gccaaataaa ggtgaagaaa gactattgat accttctcca aaggttgcaa cttatgattt
     5521 aaaaccagaa atgtcagcat atgaattgac agataaagct ttagataaat taagagaaga
     5581 taagtttgac tttatagtat taaactttgc aaatcctgat atggtaggtc atacaggaag
     5641 tatagaagcg gctataaagg ctgttgaaac tgtagatact tgtgtaggaa aattgataga
     5701 taaaatagta gagttaggtg gaagtgcaat aataactgct gaccacggaa atgctgaata
     5761 tatgttagac ccagaaacag gaaaaactgt aacggctcac tctattaatc cagtaccatt
     5821 tattgtagtt ggtcaagaat atgaaagtgc aaaattattg gatggaggaa gattatctga
     5881 tatagcaccg actgttttag atatgatgaa gttagaaaag ccagaagaaa tgactggtca
     5941 ttcacttatt tcaaaatagt cgattctgat aagtagagaa taatatataa actacaatat
     6001 aaatttaaaa ataatgcagt ttaggtttat tttgtaaagt ttagacttta tgacataaga
     6061 tttagatatt gtaaggtgta gatgaagata tattttataa aatttacaag atagacaatt
     6121 tataaaataa aaaattacaa tatagataat aatgtaaata atgatataaa taataacaag
     6181 atagacaata
//
LOCUS       DACXMZ010000215         5998 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.216, whole genome shotgun sequence.
ACCESSION   DACXMZ010000215 DACXMZ010000000
VERSION     DACXMZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.216"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            120..407
                     /locus_tag="JD016_16270"
     CDS             120..407
                     /locus_tag="JD016_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001174.1"
                     /translation="MKKFLYALYSFIVIIANFRLKEKNYNFILLAIFFILLLIQNGVL
                     KTGYQNRATFYQDNYFNGEDKNDKIKFMTIKIFIFLSLPLCFNILFNYLTS"
     gene            complement(946..1497)
                     /locus_tag="JD016_16275"
     CDS             complement(946..1497)
                     /locus_tag="JD016_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5001175.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1688..2650
                     /locus_tag="JD016_16280"
     CDS             1688..2650
                     /locus_tag="JD016_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAU5001176.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2945..4789
                     /locus_tag="JD016_16285"
     CDS             2945..4789
                     /locus_tag="JD016_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAU5001177.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVSISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            5111..5695
                     /locus_tag="JD016_16290"
     CDS             5111..5695
                     /locus_tag="JD016_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAU5001178.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2350 a          585 c          920 g         2143 t
ORIGIN      
        1 aataaaaatt aaaaaatatt taatgctatt acaataggaa ctagaactac acttaataaa
       61 tatacagaaa ttagatttgg ctcaatataa aatacaaata tagaatttag gtgtttttta
      121 tgaaaaaatt tttatatgct ttatatagtt tcattgttat tatagctaat tttagattaa
      181 aagaaaaaaa ttataacttt attttattag caatcttttt tatattgctg ttaattcaaa
      241 atggagtttt aaaaacaggt tatcaaaata gagctacatt ttatcaagat aattatttta
      301 atggagagga taaaaatgat aagataaaat ttatgactat aaagattttt atatttttgt
      361 cattaccatt atgttttaat atactattca attatttaac ctcttaaatt atatttagat
      421 ataggagtct tatgataaaa gtaagcttat gtgttattta tattaaatat aaagtgcata
      481 tagtgataaa acaaaagttt catataaatg aagaaaaaaa tataaaataa tttatttaac
      541 tacttaaaaa taaactaaaa gggataataa aatagctttt aggttatggt aataaaggta
      601 tttaaaaaga gtgcataagc actccaattg tgagaaatac atacacaaat cattattaca
      661 acaatgaaaa atgtacatat aagagacgtt ttgtatttgt tttaagtgat aatattttaa
      721 tactaactaa ataattatca caatagtaaa aaagaaaaag gagtattttg tactccattt
      781 gtcaaaaata taaaactttt atataagctt attataacat aattgataaa agtgtaaaga
      841 tatataatga atttataaag aaaaataaaa taaaaaagat aaatcctaat caaaggattt
      901 atctttttgt atagaaaata attattctat tattaaatgg catggctaaa ctggataata
      961 atttattatt actgctttca atgttttttc tccttgatta tggtatgtat gaattgattc
     1021 agctgaaaaa caaatagagt cattttctct aagttgataa atagagtcat ttacttctaa
     1081 ttttaattga ccttctataa tcataatata ttcctgtgat ttttcagaat gaccaacaga
     1141 tgtataacta tgaccttctt ctaattccat ttgaaataat tcgaagtttc tagttggtgt
     1201 attgggataa taacaataaa gacgataatc tttattttct gaaacttgta catctatatc
     1261 agtttttgat acaataaaag tttcattttg aggttgttcc agtattgcag tatatggaac
     1321 atttagtcca cttgcaattt tccaaattgt gtttactgtt gggtttgagt ctcccttttc
     1381 aatttgagaa agcattactt tacttacgcc agataattca gcaagttgac ctagacttaa
     1441 gttacgttca ttacgtaatc tatttaaatt tttagcaata acagtattaa tactcatagt
     1501 agctccttaa aatgtaaatt attgacaaat ataatttacg aatgtataat ttatttaaca
     1561 ttatttataa tttacaaatg tataatataa tgaacgttaa atacactgta catccgtaat
     1621 ataaattata ctgcataaag agttgttttt caacggatga tagttgagag attaaggggg
     1681 aattgtaatg cgtgtactta ataatgttac agaggctata ggcaatactc atatgttaca
     1741 tttatccaaa atagcagatg aatttggagt tcaaggtaat atttatgcaa agatggaata
     1801 cttaaatccg ggatttagta aaaaggaccg tgtagcatta caaatgatta ccgaagcaga
     1861 aaatgcaggt ttattagttc caggtcaagc agttgtggaa ttaacaagtg gtaatactgg
     1921 aacaggacta gcaatagctt gtgcatgtaa aggatataaa tttatagcgt gtatgtcaaa
     1981 aggaaattct atggaaagag caagaatgat gaaagcttta ggtgctgaag tagttcttgt
     2041 agaccaaatg cctaattcag ttcatggaca ggtatctggt gaagatttgg cacttgtaga
     2101 aattgaggct caaaaaatag caaaagaaag aaatgcattt agagcagacc aatttaattt
     2161 agaagcttgt aatcatgcac atgaaaagta tacagcagaa gaaatgtggg aacaattgga
     2221 tggaaatatt gatgtatttg tagattttct tggaagtggt agtacttttg caggatgttc
     2281 aaaaacactt aaaaaacata attcttctat caaatgttat gttgttgaac ctgaaacagc
     2341 agcaatttat tcaggtaaag aaataactga ccaagggcat aaaatacaag gtggaggata
     2401 ttctatggat ttgccattag tagacaagaa acttattgat ggttatattc aagttacaga
     2461 cgaggaggca actgatgtag caagaaaatt agctaaatta gaaggtgtat ttgcaggatt
     2521 ttcttcaggg gcaaatgtat gtgcagcaat taagttatta aagagcagtg aaaaaggaaa
     2581 aaatatagtt ttaactctta atgatagtgg tttaaaatat ttaagtactg atttatatga
     2641 agaattgtaa taatagcaca atagttatgc aatgtttttt aaattaagta tatagattta
     2701 aattataaaa agtatctcaa tgtaattttt gaaagttata aaggagatac ttttttattt
     2761 tgtaaatatg gagtatccta cttataagta ttaagctata tagatattga tgattagcta
     2821 tgcaataaaa aagagtatgt gtttttgcat ttatacatca taacttggaa taatacatat
     2881 taatgttcta aatgtattta aaatatatga taaaattaga acaaatagat acaaggagat
     2941 aaaaatgcaa aagagaattt taagaaattt tatatttacg ggtaaaaata ttttaagtaa
     3001 acttgttttt ataataaaat ataattttag tacattaatt ttatttgaat tatttcataa
     3061 aggattagcc ttatttttta tatggccatc tattagatat atatttgata ttttaataaa
     3121 aagtttacat atagtctacc taaatatgga taattttata aaagttttga ccaatcccat
     3181 gtcaatagtt ctgataatac tatcagttat aatactagct ttttatgcat tttttgaatt
     3241 tacctctgta attatttgtt ttaataaatc gataaaatat gaaaaaatag ggttatttga
     3301 actctttaaa atatcattta agaactctat aaaattgtta tatcctaaaa atatactatt
     3361 atttatattt gtgatattaa taattccatt agtaaataca gttttaattt cagggtttat
     3421 aggaaaaata caaataccag agtacatact agattatata aagtctgata taattttaaa
     3481 tataatctat atgatttttc ttttaataat atatatttcg gttattagat ggatttttag
     3541 cattcatgag ataactttaa atacagaaaa ttttaaaaga gcaagaaaag aaagtgttaa
     3601 tcttataaag ggaaagatta taagaacaat tatttattca ttaatattat ctttagtagt
     3661 agctattatt ggatatgcga tttatcatag tggaattata tttataggca tatggactaa
     3721 atattgtact gatatggaat ttttgaagaa tgtatttata aatagatgta ttgcatttga
     3781 agaatatttt agattgattt tatcaatatt catttttata ttggacattg gatttatctc
     3841 agcaacttat tatcaatata aaggagtaag tatttctaaa aataatatta aaaaagagaa
     3901 aaaatctata tcaaaaattg tttttaattt agtattaata ttattaattt cttatataga
     3961 gtcagttgca ttttcttcac atataaatgg tatgtttaat acgtcatttt cattttatac
     4021 aacagcaatt gctcatagag caggagctga aatggctcca gagaatactt tggcagcaat
     4081 agaagaagct tcacatgcta aagctgatta tgcagaaata gatgttcaag aaactaaaga
     4141 tggagaactt attgtacttc atgattcaaa ctttaagaga actacaggag tagataaaaa
     4201 tgtttgggat gtaaattata atgaagtaaa gacatatgat gcgggaagct tttattatta
     4261 tggaaaaggt tattatgatg aaaagtttgt tggggaaaag ataccaacac tcaaagaaat
     4321 gataaaatat tctaaaggta aggtgaaatt attaatagaa ataaaactta atggacatga
     4381 aaagggagac gtagtaaaaa aagttgttca gttaattaaa gaaaataatt ttgaaaatca
     4441 atgtatagtt gcatctatgg ataaaactat attacaaaaa gtaaaaaaac tggatgagaa
     4501 tttactgaca tgctatttta tggcaattgc atatggtgat ttttcaaagt taaattatgt
     4561 tgatatatat ggaattgaat caacttttgt taataaaaca gtagtagaaa agattcataa
     4621 aatgaacaaa aaaatcttcg tttggactgt taatagtgac agtttaattg agaaaatgct
     4681 tgacctaaat gtagatagta ttattacaga taatccatac cttatagaga gtgcaattta
     4741 ttggaagaaa aatggattta ttagtcaagt gtcagattat ttattttaaa tattctataa
     4801 ttgtattatc cagtaaaaga gatattatta aaatcaataa tatctctttt taacataaat
     4861 aaggttgtta aattaaggtt attcttataa aaattgtgct tatcagctat aaacaaggaa
     4921 taaaagtgaa aaaattgctc tgagattatt acagttaaaa gagttttaag atgacagaga
     4981 gatagattgt caactgtata tttaataaat cttgttattt attaaatttt atataagtaa
     5041 aatatataaa ttaataaatc ttatgctaga atatatatga aaaacaatat aaatttggag
     5101 gattaaaaac atgacagagc gagaaaaaat gttggcagga gaactttacg attgtggtga
     5161 tattgagttg ttaactcaat ggcataaagc aaaagaccta gtaagagatt ataaccaaac
     5221 agattcctcg gatttggaaa aaaaagaaaa aatattaaat gagttacttg gtggtaaggg
     5281 taaaaactta tggattactg caccgtatta tgtagattat ggaaataata tttattttgg
     5341 aaataattgt gaagtcaata tgaattgtac gtttttagat gataataaaa ttatcattgg
     5401 ggataatgca ctaatcgcac caaacgtaca aatatatact gcatttcatc caactaatgc
     5461 tggtgataga tttggtaatg ccaaagaaga tggttcattt gaattttgta aaactcaaac
     5521 agctccagtt actattggag ataatgtttg gattggcggg ggtgctatta tcatgccagg
     5581 tgttactatt ggagataatg tagttatagg tgcaggaagt attgttacaa aagatattcc
     5641 aagtaatatg attgcatacg gaaatccatg ccgtattatt cgagaaaata aataaagtta
     5701 aattttcttg caaaatctca aaaaatacct ttcttacttt atacataggg tattttctaa
     5761 tactttcaca tccatattca tttgttaaat ttttgtggaa tgtagttttt atcacttcta
     5821 tcttacattt ccaccatttt tttataaaag tcattccttt atagatttat ttataatagt
     5881 atatattcta tttgatgttt gaataagcac ttatttttaa gtaaaatact aacttttagg
     5941 ttagtatttt gctatagtat gatgattaag taagaataaa atactataat acataaag
//
LOCUS       DACXMZ010000216         5993 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.217, whole genome shotgun sequence.
ACCESSION   DACXMZ010000216 DACXMZ010000000
VERSION     DACXMZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.217"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            98..664
                     /locus_tag="JD016_16295"
     CDS             98..664
                     /locus_tag="JD016_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HAU5001179.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKLVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            704..1285
                     /locus_tag="JD016_16300"
     CDS             704..1285
                     /locus_tag="JD016_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HAU5001180.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1342..2259)
                     /locus_tag="JD016_16305"
     CDS             complement(1342..2259)
                     /locus_tag="JD016_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAU5001181.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIEVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2389..4020
                     /locus_tag="JD016_16310"
     CDS             2389..4020
                     /locus_tag="JD016_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HAU5001182.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4023..4970
                     /locus_tag="JD016_16315"
     CDS             4023..4970
                     /locus_tag="JD016_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAU5001183.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5255..5518)
                     /locus_tag="JD016_16320"
     CDS             complement(5255..5518)
                     /locus_tag="JD016_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HAU5001184.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5617..5862)
                     /locus_tag="JD016_16325"
     CDS             complement(5617..5862)
                     /locus_tag="JD016_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001185.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         2154 a          822 c          980 g         2037 t
ORIGIN      
        1 tagccaactt agcctaaata cgtatataat atagctaata tggttcttat acttgatacc
       61 atcaataaaa ctaaaaatat aaaaaggaga acttaaaatg agtgttagtt tacaaaaagg
      121 acaaagagtt agcttagtaa aagataataa tcctgtaaaa aatctagtag taggcttagg
      181 atgggatatg aataaattgg gtaaaaaaaa ttatgaccta gatgcatttg caatagcatt
      241 gactaaccaa gataaaatga atgttagagg tgacttggtt tattttggaa atcttaaaca
      301 cccttctaaa gctattatac acactggaga taacttaact ggaaaaggtg aaggtgatga
      361 tgaacaaata attgttaatt tagaagacat acctgaatat gtacataaat tagtttttgg
      421 agtttctata tacaaagcta aaaaaagaga ccaggatttt ggtcaaatga acaactcttt
      481 tataagatta attgattcaa attccaagca agaattattt aaatataatc ttcaagaaga
      541 cttttctggt aaagtaacag ttttggcagg agaaatttat cgtagaaatg aagaatggaa
      601 atttaatgct ttaggaattg gtcaaaatga agagcttcgt gaattaatta atacatataa
      661 gtaataatta caataaaaaa tttataatga ggagttggtt taaatgggta taactttagc
      721 aaaaggacaa aaagtaagtt taacaaaaag taaccctggt ctaaaaaaag taatagtagg
      781 actaggatgg gatattaata aatatgatgg tggctttgat ttcgatttag atgcctctgc
      841 atttttaact ggaactgatg gaaaagtaac gaatgatgga gactttatat tctataataa
      901 tttaaaacac gcttctggag ctgttgaata tatgggtgat aatagaacag gtgttggtga
      961 tggtgatgat gaacaaataa atgtagactt atctaaaata cctcaaaata ttgctaaaat
     1021 atcatttagt gttactataa atgaagcaat tacaagaaga caaaatttcg gacaagttga
     1081 aaattcatat ataagaatat ataatgaaga aactaatgag gaacttatta aatatgaatt
     1141 gggtgaagat tttagcatag aaacagcaat agttgtggca gaactatata gacataatgg
     1201 agagtggaaa tttaatgctt taggttctgg atttgaggat ggacttgcag gattgtgtaa
     1261 aaattttggc gttaatatag gctaatatat ctaaataaaa ataagagtat attgatatac
     1321 tcttattttt atttagatat attataaact gtttacactt ctactcttct ttctcgcttg
     1381 ttttttccca tattctactg aaatatcaaa aaatgctgaa atattttctg taaaaaactt
     1441 gcttctatga tatgcaatat caaatgtcct atgtaaatta ataccttcaa tatcacaagc
     1501 ccacaaaaga tgctttttta aataatcctc aataagtaat tcagatataa ctgcaatgcc
     1561 aaaattatct acaactgctc ttaatatagc ttcagagtta taacatgacc atctaatatt
     1621 cattgggatt tttaattctt ttaattgctt ttctaattgt gctcttgtac cacttcccaa
     1681 ttcacgcata attaatggtt gatttgaaag ttctgaaact tctatagagt ctcttttata
     1741 aaactcatgc ttgtgtgaac atataagtac aagattatca ttaataatgc tttttgtaac
     1801 aatatctggg tgcttaattt ttccttctac aatcgcaata tctaattcac tattaagtaa
     1861 tttttttgca atcagtcttg tatcttctac aaatacttca ggttctataa gaggatttac
     1921 cttgtataat tctaacatta tcgggcttat tacacatgta ccaacagtta ctgttgcccc
     1981 aactcttata cggatatttt ttgcacaatg cttcatacaa tcattcattt cttcatttac
     2041 agctaaaaga tgcctcccat atccaagaag ttttttccct gcctcagtta aatataattt
     2101 ttttgaaatt ctatcaaata gcttaacacc atactctttt tctatttctg aaatagcttg
     2161 gctaactgat gactgggtaa tatacatatt tcttgctact tcactcattt taccacattc
     2221 agcaacctta acaaagattt caagactacg aattgtcata ttatcacaac cttagcaaac
     2281 attagtaaat gcttatgatt ttattaatat tttagtattt tacaaatata ttgtcaagaa
     2341 gtataatttt attatagagt tataaagtag attggagtgt gatatattgt gaaagtatta
     2401 attataggtg gagttgctgc tggaacaaaa actgctgcaa aaataaaacg tgaaatgggt
     2461 gataattgct ctgttacaat actaaataaa ggtgatgata tttcttatgc aggttgtggt
     2521 cttccatatt acgttggtaa gataattgaa gataaatcat ctttaatcgt aaatacacct
     2581 gaaagttttt ctaaactaac aggagcagaa gttcgttgtg gagttgaagt tacatctatt
     2641 gatagaaatg caaaacaagt aactttgaaa acgctttcaa caggagaaaa agaaaatcta
     2701 agctatgaca aacttgtaat tgctacaggg gcagacccaa taaaaccacc aatacctgga
     2761 atagatttac ctggagtatt ctttatgcgt actccaaatg atgctataaa actaagagat
     2821 tctatcgaaa acggtattaa gagagctgtt gttattggtg gaggtttcat tggtcttgaa
     2881 attgctgaaa atctttctgc aatgggtata cgtgtaagtg ttgttgatat ggctgaacat
     2941 gttatgcctg gatttgatac tgattttgct gaatacgttg aaaatcatat ggctgataaa
     3001 ggcataatga tttttacagg tgaccaggtt gtaggtattg aaggtgaaaa taaagttgaa
     3061 aaacttcgta ctaaaaatag agctattaaa acagatttag tggttatgtc agttggaatc
     3121 cgtcctaaca caggttttct aaaagacaca ggtttagaat ttgcccctaa taatacaatt
     3181 attgcagatg aatatatgct tacaaatgac aaagatatat acgtagttgg agactgtgct
     3241 tttgtaaaaa attctttaac aaataaacct acatggtcac ctatggggtc atctgcaaat
     3301 catgagggta gaatttgtgc acaaaatata tctggaaaat ctaaaactta caatggtgtt
     3361 cttggaacaa cagtagtaaa attacctgag ctcaatgcag gaaaaacagg tctaagtaaa
     3421 gaaatagctg aaaaagaagg ttataatgtt tgctctgtaa ccatagctac tgatgataaa
     3481 gctcattatt atccaggtgc atctaatttt attataagaa tggttgctga aaaaaatact
     3541 agaaaattat taggtgttca agttatggga cctggagcgg ttgataaaat agtagatatc
     3601 gctgctactg caataacttt aggcgctgac ctttattcat tagaaagtat ggaccttgca
     3661 tatgcaccac ctttttcaac agctattcac ccttttgtag tggcagtaaa tgttttacaa
     3721 aataaactta atggagaact tgatagtgtt ttacttgacg aaattgagga ttttgacagt
     3781 tggactaaac ttgatgtttc aaaagctcct tctattccta gtttacgtta tattcctgtt
     3841 ggtgaaatta atggagaaat tgaaggcctt gctaaggatg aaaaaattct tcttatatgt
     3901 gctaaaggta aaaatagcta tatggcgcaa aatcgcttaa gacgctttgg ctatacaaat
     3961 acgaaagttc ttgaaggtgg aatactattt tatccaaatc ttaaggtaat ggaggagtaa
     4021 atatgagtaa tgttacagca gaacaaaaaa aggaagtaaa aggtagaggt tttctaccaa
     4081 gcagggatgg agaacatttt gcagcacgta taataactgt aaatggtgta ataaatgcat
     4141 cacaagcaaa aaaaattgca gaagctgctg aaaaattcgg aaacggtcag ttagcattta
     4201 ctactagact aacagtagaa ttacctggtg taaaatttga agatattgaa gctctaagtg
     4261 aatttattgc tagcgaaaat ctaataacag gtggaactgg atcaagggtt cgtcctgttg
     4321 tggcttgtaa aggtactgtt tgtgttcatg gtctagctga tacacaggca cttgctgctg
     4381 aaatacacga agaattttac aaaaattggt atgatgtaaa actacctcat aagttcaaaa
     4441 ttggtgttgg tggatgccct aataactgta ttaaacctga cctaaacgat cttggaattg
     4501 ttggtcaacg tgtacctgac tatgattcag agctttgtgt tggttgtaaa aaatgtgctg
     4561 ttgtagaggt ctgtcctgtt aaagcagcta aattaacaga taaaggtaaa cttgaaatag
     4621 atagtaatct atgtaataac tgtggtaagt gtatagaaag ttgtaatttt gatagtattg
     4681 aagaaaaaga atctggttat aaagtctata taggagggaa atggggaaaa tcagttcgtc
     4741 ctggaacaca gattgataga ttattttcaa aagaggaact tatgacttta attgaaaaag
     4801 caattctttt atttagagaa cagggtaaca caggtgaacg ttttggaata actatcgata
     4861 gaatcggtgc tgataaattt atagaaatgc ttttatcaga tgaagttttg gaaagaaaag
     4921 aagagatact agaggctctt ttacacctta caggtggagc tgtatgctaa caataaatat
     4981 tttaattgta cagataacct atggaaaaaa ttttagttaa gaataattaa aattctatat
     5041 atgatatatg ataaatgagg ctatctcaaa acacagatac gcctcttttt cattgttgaa
     5101 atgataaagt atatttaagt ttatacaaaa aagagtgtct cgtgaattat aaaattcatt
     5161 ttgaggcacc cacattattt tataataatt gcttattaat tttattttaa ctatataaaa
     5221 agtacaaaaa gtaagaatat ccaatattct aaaattaatt ttgaattgta ttgattgaaa
     5281 agtttactat tccactattt ccatcttgat ttactactag ataccatgtt ccagtattag
     5341 gaactctcat tctacatgga tttttctttg caaatccacc atagtaatca taatttcttt
     5401 gatttttaaa tttcacataa tttatatggt ctaacaatat tacattggct tgtttctcta
     5461 aatttacctc tacaatatcc ccttttttta agttcatctt actataacta tattccattg
     5521 aattactcct ccagtatcga ataatctttg ttatttactc atctcaaata tgtaaaaatt
     5581 catttaaaat attacaaata ctatataatt agactattat ccagttttaa ccttatttac
     5641 tacaatccaa aggttattaa aaatattatt ggcaacaact tctccatccc atacaactct
     5701 ttcttcttta ttttctaata aagcatagta cccagagtca gaactttcat ctttatagac
     5761 tgtaacataa aactcttcat cactatattc aggtatattt ttaaagtctt cagatatttt
     5821 acatgttaat atataacctt ctaattcttc atctataaac aaagcaattc ctccaatctc
     5881 tatattttta ctaattatac catattatgt cataaacttt ttattttatt acaaatttct
     5941 attttttctt tactttataa ataaattata taaattttaa ctttttaact tta
//
LOCUS       DACXMZ010000217         5993 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.218, whole genome shotgun sequence.
ACCESSION   DACXMZ010000217 DACXMZ010000000
VERSION     DACXMZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.218"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(139..546)
                     /locus_tag="JD016_16330"
     CDS             complement(139..546)
                     /locus_tag="JD016_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001186.1"
                     /translation="MSKVAKAAFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1020..1910)
                     /locus_tag="JD016_16335"
     CDS             complement(1020..1910)
                     /locus_tag="JD016_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAU5001187.1"
                     /translation="MIMDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEE
                     VELAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKY
                     DYKKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLRE
                     PTIDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIH
                     EVEQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIE
                     AKAIRKLRHPSKLKQLKHFY"
     gene            complement(2195..2671)
                     /locus_tag="JD016_16340"
     CDS             complement(2195..2671)
                     /locus_tag="JD016_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001188.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            2938..4113
                     /locus_tag="JD016_16345"
     CDS             2938..4113
                     /locus_tag="JD016_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HAU5001189.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4194..4997)
                     /gene="proC"
                     /locus_tag="JD016_16350"
     CDS             complement(4194..4997)
                     /gene="proC"
                     /locus_tag="JD016_16350"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAU5001190.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5037..5621)
                     /locus_tag="JD016_16355"
     CDS             complement(5037..5621)
                     /locus_tag="JD016_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU5001191.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         1996 a         1020 c          675 g         2302 t
ORIGIN      
        1 tttagtttga ttagctttag gggtttccag caattcaagt agtattggat aggttctatc
       61 ctatctctac atataggttt ccctatatgc tgactatttt agctactaac tcatgactaa
      121 agtcacgagt gtgtgtagct attttttaat caatatttta gtaaatttta cggtaagtaa
      181 aaacctctct ccttcaaatc ctatagcaaa tatacttaca agttgctttg agaaaataac
      241 tcctaatata gctacaacta tgcatatacc aactattata tttaaaacat tgtttaaaaa
      301 ctttagtgct tgcttttcac cttgtttact acttatatct tgatacattg gaataaaagt
      361 agtaactata gcagtaccta ttgctgcaaa tataatattt ggtatattca tagctgttaa
      421 ataactctct gtatataagc ctgtaccata aattgatgat aatactaatt ctctacccat
      481 tccaagaatc ttagagatta tagtaactat catcaaataa aaagctgctt tagctacctt
      541 gctcatttta tcactccact tctaacatac ctctttttaa tcaagtaaaa tttagctcat
      601 tagatataat tctatcaact aatttgcctt gcttagccct atcaaaatat aattaacacc
      661 tatttcttaa tatatcacaa actacattat tcttcatatt tttttatttc tccaataagt
      721 tttacatttt tcctatataa attgtatagc ttcaattata ttcaaagaaa tggagttgct
      781 acccgataac tttaaaagta aaaaacagat tttattatct ttttaaacta tattctaaca
      841 tattttgtcc atattgagaa attttactaa actattatgt actttattaa ataaataaaa
      901 taacagcaca cctataaaat acaagtgtgc tgttcataaa cagattttat aaaaaaataa
      961 ttcctaagta gagattaaca tgctttctca tactctcata tataatgtgt ataaaaatat
     1021 taataaaaat gctttaattg ttttaactta cttggatgtc ttaattttct tattgcttta
     1081 gcttctatct gtcttattcg ttctctggta actccaaata cctttccaat ctgttctagt
     1141 gttttagggt catcatcatc aattccaaat ctcattctta aaacttgctg ttcttgctct
     1201 ccaagacctg taagtagttc ttctatcttt tcttttaaat tatgctgttc aacttcgtgt
     1261 attacaacat ctttaaaatc agcatctgat ggtataaatt caactaaact actatcctca
     1321 tcttctttta atggagtatc caaagataca acatttttat ctcttcttaa taattccaac
     1381 actttgtcaa cttctaagcc acatgcatct gcaatctcat ctatagttgg ctctcttaat
     1441 aatacattta aaagttcttg tttctttttc tttacaaaat ttattctctg atgaagatgt
     1501 ataggtatcc taatagtatt ttcgcaatcg tcgatatatc ttgttatact ttgttttatc
     1561 caccaagttg cataagtgct aaatttgtat ccttttttat aatcgtactt ttctacagct
     1621 tttataagtc ctatatttcc tgcttgaatt aaatccaaca tatcaatact atctctttta
     1681 tatctctttg ctatgctcac taccaaccta taatttgaat ttataaattt ttcttttgct
     1741 actgaagatg agttgattat ttccttagcc agctctacct cttctcctgc tgacaacatt
     1801 ttgtattcct ctatttcctt taaatacatt ttcatcgcat ttgatacatt tgatgaaccc
     1861 acacaaatat aattttcata attattttct tttttagtat ccataatcaa accccttcta
     1921 ttcatagaat ttgctaaaac aaaaataaat aatttaaacc tacataatct acaattacat
     1981 aatatcccat tctttaaatg aattgttttg tatattatgt atacaaattt ataaaaacta
     2041 tacctataat attattctac ttctaacagc aatatcctcc ttatatatat aatttatttt
     2101 attatatgtt gttatattct tagttttttg aatgatatat gtctaaaaaa agactgttta
     2161 ctaaacagtc tttttttata tatatgctca atatttaatt ttcatgatga tgattacatc
     2221 cacatccaca attttcatca tggtgatgat gttcttctaa atcttttacc tgttgttcca
     2281 tttcatgaaa ctcttttacc attatatcat aagttggaag agctgcctca gtatcgtatt
     2341 cttcccctct ccaatcagcg tacatcatag catctccatt agttaaaacc aatctacctg
     2401 acatagttat attatccact tcttctaaaa attctatttg tttttgtaaa acttctcctt
     2461 ctaactccac atcttcatct aaacattgca ctatcattac aggtgcataa taatcttctt
     2521 catctttaac atttacaaca atatctaaaa ttccttctgc accttcttca aattctgcca
     2581 actcaacatt tgtatcaatc atatcttgag gtactaataa atcttctatt aaatatttta
     2641 ttactctatc ttttactaat tcttttgaca tttatttttc tccttccgat tctataacat
     2701 tatcataatc atttctatat tatagtatac tacattaatt gttttttcaa tctcttacaa
     2761 aaattatttt ttcattatct tccttatatt ccctttttta ttacaaagtt gtaatcgcat
     2821 tgatttaaaa aatagtttat atttagtata atagaaatgt agcacatatt tttattcaat
     2881 atattaataa aaaaatagat tgaaaatgta atgtatgtta aatctaggag gaatattatg
     2941 aagtttaaaa aacaaattag ttctattgtt gtgtctacta tgcttgtact tgggagtatg
     3001 aacctttctt atgcagacgg tacaaatgta gttacacttg gagctaactt atcagagtct
     3061 caaaagcagc aaatgcttaa ttactttgga gtaaaaaaag accaagtctt agttttagat
     3121 gtaacaaatg cagaagaaag acagtattta caaggtgttg caacagaggg tcaacttgga
     3181 aaagttacta tctcttgctc atacgttgaa cctactaaaa aaggaaatgg aattaatgtt
     3241 aaaacagcaa acttaacttg ggtaactagt tcaatgattg cttcaaccct tacaacagct
     3301 ggtcttgaag atgcaaatgt tattgctgct gctccatatc ctgtaagtgg tactggagca
     3361 ttgacaggaa tcatgaaagc atttgaggat gcttcaggaa agaaacttga tgaaactaag
     3421 aaagaaattg cttctgaaga attggtagtt acaggtgact taggagatga tatagggcaa
     3481 gaaaaagcaa caggtgtaat gaatgatata aaaacagaaa ttgtaaagaa tggtacaaaa
     3541 gataccaatc aaatagcaaa tacaataaat aatgttgtaa ataattacaa tattactatt
     3601 acaccagaac aaaaagaaca aattaaaggt gtaatgaaaa aaatagctga ccaaaattac
     3661 gattacaata gcatgaaaaa tacactagat aatgtatcag ataatgtaaa tgaacaatta
     3721 aagaaattag gtgaaagtgt taaaggttct ggtatactag atactattgg tggatggttt
     3781 agtggaatag gagactggtt ctctggttta ttctcaggcg gagataaaaa agatttggga
     3841 atcttaaata acactaatga tgagtctctt ggctctgatg ctgtaataaa ttctacagaa
     3901 ggtgttcaaa taaatccaca aaataatact aattctaaca atgatagtga tacaaatagc
     3961 tcaaaaaatg atacttcaag taataatgaa aataacgata gttctcaaac aaatgaaaac
     4021 aacacaacaa ataattcaga aaatcaatca aataataatg actcagttgg tttctttgaa
     4081 aaaataaaaa attggctttc aggcttattt tagaaattaa acaaataaaa agaattgcct
     4141 aaaatggaaa ttttctataa aaggcaattc tctttattta caataaattt ttattactca
     4201 ctcatttttt tagatttttc tatacatgca catattgctt ttatcacaga agctctaaaa
     4261 ccttcttctt caagtacttt aactgcttct atagtagttc caccaggtga acataccata
     4321 tcttttaatt caccaggatg ttttcctgtc tcaagtacca tttttgcaga acccataaca
     4381 gcctgtgatg caaacttata tgcttgtggt cttggcattc ctgcaattac tgctgcatct
     4441 gctatagctt ctataaataa aaatacatat gctggtgcag aaccacttgc accaattaca
     4501 gcttctataa gatactctgg tactacctca gtatttccaa aagaattaaa tacttctgta
     4561 actgcttcta agtcttcttt gtttatattt ttatttatag aaagagaaga catggcttca
     4621 tttacaagag ctggagtatt tggcatagtt ctaactattt ttttatcttc tcctataata
     4681 gactctatat cttttataga ttttcctgct gctattgtaa ctataatttt attgtcatct
     4741 ataaagtctt ttatctcttc caaaatatca tcatatatat ctggtttaac agctacaatt
     4801 actatatcag aggttctagt tacttccttc gagtcttttg tagtatttat tccaaaattt
     4861 gcatctattc tatcaagagt tccttgacta tatgctgatg ctataaccat attaggttca
     4921 actaattttg aatttactat tccccctatc atagcgcttg ccatattccc tgcacctatg
     4981 aatccaattt ttttcacttt agtacctcct tattgactaa tagtaatcgt taaaatttaa
     5041 gcttctttaa ctgcatcaga cttttcaaat ggatttggta tacttactac caaaattttc
     5101 actaccttat tagtatagtt gacccaatta tgattgttct tttcactatt atagtgtatt
     5161 gtgtctcctg gatacatttc tatttgttca tctcccaaaa acactgtaag agtaccctct
     5221 aatacataca caaactcctc accttcatgt acgtaacaac aaatttcctc actactctta
     5281 cttggaagaa tttcaattaa tcttggtagc attgtttttt cctttaaact agatgataaa
     5341 tgataatgta taaatgttga gttttctaca tcaaatactt ctttttcata acttcttaat
     5401 acagctcttt tatgctcttt tggcttcata aaaaagtaag ttaattctac atctagtaca
     5461 cttgctattt ttcctaagga atctgttgcc acacttgtta atcctctttc aagttgtgat
     5521 aaaaaaccaa ttgacaagtt tgttttttca ctcatatcct tcagcgtcat ttgcttttga
     5581 gttctgagtt gctttatttt tgctcctatg tccttattca aaaccatcac ccctaaataa
     5641 ttttaatttt ttcataatat aaatacaaaa atataaatat aggtactgaa cctatcttta
     5701 tttcatttaa aaaaattttt ataagtattg ctataattta tcaatctttt tttgtctaca
     5761 tttttacgat tatttacgat ttatttttca catatgtata tttaatcttg ccaactaaag
     5821 atacattttt tataagcatt tactctttat ataataaaac ttaagttgtt catgagtctt
     5881 cttctggttt aattattaaa atttcaaatt atattattat ttctacacat tattacatcc
     5941 tttagtacaa ttataacata attttacaat ttaatgatat ctattcaatg tct
//
LOCUS       DACXMZ010000218         5971 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.219, whole genome shotgun sequence.
ACCESSION   DACXMZ010000218 DACXMZ010000000
VERSION     DACXMZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.219"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            125..937
                     /gene="modA"
                     /locus_tag="JD016_16360"
     CDS             125..937
                     /gene="modA"
                     /locus_tag="JD016_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAU5001192.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            947..1738
                     /locus_tag="JD016_16365"
     CDS             947..1738
                     /locus_tag="JD016_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5001193.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFLSLTCLMLFEIFFNREVDKN"
     gene            1740..2435
                     /locus_tag="JD016_16370"
     CDS             1740..2435
                     /locus_tag="JD016_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAU5001194.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2422..3153
                     /locus_tag="JD016_16375"
     CDS             2422..3153
                     /locus_tag="JD016_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001195.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNSHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            3467..4267
                     /locus_tag="JD016_16380"
     CDS             3467..4267
                     /locus_tag="JD016_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HAU5001196.1"
                     /translation="MTFKNKKFTINKYEILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISIFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            4807..5523
                     /locus_tag="JD016_16385"
     CDS             4807..5523
                     /locus_tag="JD016_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5001197.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            5504..>5971
                     /locus_tag="JD016_16390"
     CDS             5504..>5971
                     /locus_tag="JD016_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAU5001198.1"
                     /translation="MKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSNINYYY
                     REFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVKFLENV
                     ISLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIYE"
BASE COUNT         2321 a          724 c          844 g         2082 t
ORIGIN      
        1 ttcttaacta tacataacaa aacataacaa aacattataa aacataacaa aaacttagac
       61 atcgttttct tagtatgata tactaaaaaa gaatttacac taatttaata agggggaata
      121 tcttatgttt aaaaaacttt cagtactagc tttagctatg attatttcat taagtattac
      181 tgcatgtaat tctaaagaaa acaaagctaa tgatgaatct aatactaaag tgacaacagc
      241 tgatgattca aaagaaaaag agactctttt tgtctattca ggggcaggtt taaagaaacc
      301 aatggatgaa atagcaaaaa aatttgagaa agaaaataat gtcaaagttg aatatagcta
      361 tgcaggttct gcacagctaa tagctcaaat tgagacaagt cacaaaggag acgtatttat
      421 tgttggttct gaaccaattt attcaaaggc ctttgaaaaa gaacttgtag gtgaacataa
      481 aacggttgca catcatacac ctgcaatagt tgtacctaaa ggaaatccag ctaatattca
      541 aaaattggaa gacttaaata aagatggagt taaacttatt ttgggagaca aagagtctaa
      601 tgctataggt aagactacac aaaaaatact tgaaaaaaat aatttaaaat ctataaatga
      661 taatgttgtc tctacagctg caactgtaaa tgaaatgatt gtacagttaa catcaagtaa
      721 agctgatgct acaatagcca caaaagattc ggtctttggt aatgaagatg tagaagttat
      781 agaaattgcc caagataaaa atatagacca actcatatca gcaggaatcg taaactatag
      841 tgataaaaaa gatttagcta ataaatttat aaattatata gagtcagacg aagctaaaca
      901 gatatttaaa aaatatggtt ttgaacctgt taagtagtga ttaaacttga aaaataaatt
      961 ttttactact ctacttactg gattttttgc tatagtcttt ttgtttattg tatcaccact
     1021 tcttatgtta atcattaaag ggataagtta tgttcctata tgcttaaaat cagtggaagt
     1081 acagtatgca ataacactta gtattaaaac atctttaata tctactatag tatgcctttt
     1141 attagctatt ccagtagcat attttttaca tattactaaa ttaccattta aaaaactgat
     1201 tatacagata ataaatctcc ctatgtctct tccacactta gtatcaggaa ttgctctttt
     1261 acttttgttt ggaagaatgg ggattggaga ttccatttat aagattttta agcttgattt
     1321 tatatttaca aagcaaggta tagttttagc acaaatattt gtaaatcttc cacttactat
     1381 taaaatactt cacacttcat taaatgaaag taatgaaaag atgatatttg ttgcacgtac
     1441 ccttggatgt aatagttggg aagcatttag atttataatt cttccaaatc taaaaactgg
     1501 aatcatatct gcaactgtaa tgacttggtc aagagcactt ggtgaatttg gtgcagtagc
     1561 tatgatagca ggttctacac gtatgaaaac tgaaattatt cctacatcta tatatttaaa
     1621 tatgtctact ggtgatatag acattgctat cggaatagct gtaattttga tatttctctc
     1681 acttacatgc ttgatgcttt ttgagatatt ttttaataga gaggttgata agaattaata
     1741 tgttaaaaat aaaaaattta tctcttgatt tacctaattt taaattacaa gaaaatagtt
     1801 taaatattaa taatcatgaa tactttgttt tattaggagc tagtgggtca ggtaaaactc
     1861 tatttctaga aacattagca ggaagatatc catctgctaa aggaaatgta tcatacaaag
     1921 aaaatttaat atcaaactta cctccagaaa atagaaatat aggatttgta tatcaaaatt
     1981 ttgaattatt tccaaatatg aatgttgaag ataatataag gtttccactt aaattgagaa
     2041 aaatacctat agatgaacag attcttaagg ctaaccactt gtctaaatta atgcagatag
     2101 aagatataaa aaataggtat cctaaaaatt taagtggagg cgaaaagcaa cgagttgctt
     2161 ttgcacgtgc cttaatagct gaacctgata tattactttt agatgaacct atgagttcat
     2221 tggattatat tacaaaaaaa catataagca aaattttgaa ggatgtctac ataaaataca
     2281 aaccaacagt gattcatgtt actcatgata tttctgaagc aatgtttttt gctcataaga
     2341 taggaataat gaacaatgga aaaataaatc atatttttga tttaaatgaa gaaataaaaa
     2401 ataaaggaga atcttttttc tatgaatata tttgatattt tgagagatta tctattagta
     2461 caagtcgata aatataattt aaatatggat atgataagta ttgtgtccaa atctctatct
     2521 tcaaaagaag caattggaaa tactaagcgt aaagatttcc caattatagt tggtaaagaa
     2581 ataatgcttg aagctgactt taaaggagct aaaggacaag catttacttc tactccatct
     2641 acatttgaag gttctttaaa agacatatta tcattagatt tacatgacaa ttctcatgat
     2701 agaagtcttt ttatagcatc attaaatgca gttatgaaat acttaggcaa gacggataga
     2761 accattcatt gtaaaaataa cgagccagag gtatgtgcaa aaaaattccc tgagtttata
     2821 aaaatggaat ttggtaatcc aaaagtagca attataggat atcaaccagc cataattgat
     2881 aatattaagg actttttcga aacaagagta ttagatttaa atcctgaatt tgtggatact
     2941 attcaatata atgttaagat tgaagatggt atacgagatt atgaagatgt aatatcttgg
     3001 gctgatttag tgatttgcac aggaagtaca ttatgtaata actcaattat aaattttcta
     3061 tcacttaaca aaccagttta ctattatgga actacgatag ctggtgcatc aaatatttta
     3121 ggtttaaaaa gactctgttt ttgtagtaaa taagcaatca tatcatcaag gagtacacaa
     3181 atatatatgt gtactcttta aatttaaaaa gttattactt ttattctaag tactaaagta
     3241 aaattctatt aaaatgaatc ccgaataaat gtacaatccc tttaaatctc aattcctatc
     3301 aatcattaat attttgatta gtcttataca taaatgtttt ctttatttta aactaataaa
     3361 aaaattttat tccattaatc tactctatat ttttcagcat ttaagaaata atttttagta
     3421 ctttgtttga taatataact aagacaactt ttcaggagat gattaaatga catttaagaa
     3481 taaaaaattt actataaata aatatgaaat tctatctatt attctagttt tatgtttgat
     3541 tttaaccgct tttagtagct atgtgaatta tagtcataaa aaatttaaag attatgttgt
     3601 cttctatgtt cagcatcaag atgatgaagt gttgtgggct gggagtgcta ttgttaatgc
     3661 tataaaagaa agaggaaaaa atcatgtatt tgtggtttta gtatctactg gatgtggtat
     3721 atccatcttt gataaatata aaaaatatga aaacctaaca aatatacaaa aatcagaata
     3781 tagaaataga gaatttttag cttctcttaa tagccttggt gtaataaggg aaaatataat
     3841 attattacca gaaacaaata caaatggtaa tactgacttc aattacatgg aaaaaattgc
     3901 tcttacattt gaagaaaaat ttaaaaatgt aactcatata gcacatactt ataagttaga
     3961 tgaccattta caacatttaa aaaatggttc tgttatccaa aatttatata atgctggtaa
     4021 aataaaagat gctaaatatt ttgtaaaacc taaatttgct gataaaataa cttttaataa
     4081 taagatagtt tataaagctc attctactga agactatgaa aaggttaaaa atgcctgtca
     4141 acaatacaaa attgttgatg aattagaata tagagaaggt attggctata agtctgacca
     4201 taaatcattt gataagttat taaataataa agatgttcct gctatacttc atacacctaa
     4261 tttataagaa atatttattc ttttaattta agttataaaa aggaaattat ttgagatttt
     4321 gtgatatagc actttttgtg ctatactact ttttctcata caactattac ccatatctaa
     4381 tttcatcatc tcttaaaata tataaagata tcatcattaa aacctataag ccttattaaa
     4441 tgctttatac atacctacat taaactacct gtttatatta aactacatta aaataaatat
     4501 ttgttataaa ttcttaataa ttatgaaact catttgttat ttatatttgt taatatatgt
     4561 ttaaacctaa aaaattaata aaccaaaata acataagaag tgtgatagat taccctagat
     4621 attttcataa acttttacct tcagattaat atgggcgtct atacttttaa atttactata
     4681 tgcattgaca atatattaaa agtactagtt tttatttcat aatgaatggg cttactgttt
     4741 tctaatacct ctttttggta taaaataaaa attggttaaa aataaaattt caagggggtt
     4801 aataagttga atcaaaatat attggttgta gatgatgata gagagatagt tgatgctata
     4861 gagttctatt tgagacctga taactttaat gttttaaagg catatgatgg acttgaagca
     4921 ttggacatac taatcgaaaa tgatattaag ttaataataa tggatgtaat gatgcccaat
     4981 ttagatgggc ttaaaactac acttaaaata agagaaaaaa ggaatatacc aataattctc
     5041 ttatctgcaa aatcagaaga tatggacaaa attataggtc ttaatatggg tgctgatgac
     5101 tatataacta aaccatttaa tccactagaa cttactgctc gagtaaaatc tcaacttaga
     5161 aggtatataa atttaggtga ttttagtaat acaaataact caaatgatac taattatatt
     5221 ctaaaaagtg gtggcctaga gctaaataca gatacaaaaa tagtttcttt agatggtgag
     5281 gaaattaaaa tgacaccact tgaatataaa atacttacac ttcttatgtc acgaaaaggt
     5341 aaaattttct cttctcatga gatttatgaa aatgtctgga atgaggatgc ttatagttgt
     5401 gagagaacta ttgctgttca tattagaaga attagagaaa agattgaaat taatccaaaa
     5461 gaaccaaaat atttaaaggt ggtgtggggc attggttaca aaattgaaaa acttaattat
     5521 taatgattct aaagcaaaat ttattttatt attattactg tttctattag tttctatatt
     5581 atttgaagca gtttatgatt tgaataataa cttttctaat attaattatt attatagaga
     5641 gtttgctctc ccttattttt attttttcgg tactcctgta catttgtcaa aagtagaatt
     5701 agagtttgtc tgcataggta ttatattatc aagtttttta ttgactatac tatgttttac
     5761 attgtataaa aaaaataaac aaactaaagt caaattctta gagaatgtca taagcctttt
     5821 ttctaaggta aaaatagaac ttacacttat attaactttg atactattaa ccttaaggtt
     5881 tcatgcattt acaagtaata aaatgtttta caacactaat ttaataatct acaattttat
     5941 aattatatct atagtctata taatatatga a
//
LOCUS       DACXMZ010000219         5958 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.220, whole genome shotgun sequence.
ACCESSION   DACXMZ010000219 DACXMZ010000000
VERSION     DACXMZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.220"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            196..1560
                     /locus_tag="JD016_16395"
     CDS             196..1560
                     /locus_tag="JD016_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAU5001199.1"
                     /translation="MDIKELVKMQRKYYNTSKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKMM"
     gene            1720..2886
                     /locus_tag="JD016_16400"
     CDS             1720..2886
                     /locus_tag="JD016_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAU5001200.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            3003..3785
                     /locus_tag="JD016_16405"
     CDS             3003..3785
                     /locus_tag="JD016_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAU5001201.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3904..4557)
                     /locus_tag="JD016_16410"
     CDS             complement(3904..4557)
                     /locus_tag="JD016_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAU5001202.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4581..5945)
                     /locus_tag="JD016_16415"
     CDS             complement(4581..5945)
                     /locus_tag="JD016_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HAU5001203.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMLKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2234 a          752 c          906 g         2066 t
ORIGIN      
        1 ataattttaa aaaatgttta ttttaaaata aattatttta taagcttact tatacttaat
       61 aaggggcttt ttttattgga agatttatgt tatattgaga tgaatttatt atgatataaa
      121 aaataatgtt aggataaatt taaatctttt tctaaaaatg tattaatata attcttaaag
      181 aaaggatttt taataatgga tataaaagag ttagttaaaa tgcaaagaaa atattataat
      241 acaagtaaaa caagagatat atcgtttcgt atagaacagt tgaagaaact taagttagtt
      301 gtatcacaaa atgaagaaaa aatactttta gcactaaaga aagatttaaa taaatcggat
      361 tttgaaggtt ttatgacaga agtaggcatg ttttatagtg aattaaactt tgctataaag
      421 aatataagaa aatggagcaa aataaaaaga gttaaatcaa gtatggttaa ttttccatct
      481 atatccaaag ttgttccaca accttatggt gttactctca taatgtcacc ttggaattat
      541 ccatttcaat tagcacttat accattagtt tggtcattag cagcagggaa ttgtgttata
      601 ttaaaacctt ctgaatattc tgtgtcaaca tctagtgtag ttaaagacat agttgaagat
      661 actttttcaa aagaatatgt tgcagttgtt caaggaagtc aagaagaaag tgaaaagtta
      721 ctattggaaa gatttgatta tatatttttt acaggaagta ctaatgttgg taaaataatc
      781 atgaaatcag catcagagca tttaactcct attactttag aacttggagg aaaaagtcct
      841 tgtatcattt taaaagatgc taatatagac cttactgcca aaagacttac ttggggaaaa
      901 ctaataaatg caggacaaac ctgcgtggct ccagattatg ttttagtgca tgaagataga
      961 aaaaatgagt taatagagaa aataaaatat tatactaata agtattttgg ggataatcca
     1021 tgtaataatg agcaatttcc taaaatcata aatcagagac attttaatcg aatattatct
     1081 cttattgata aagataagat agtctatggt ggaaattata ataaggagac attaaagata
     1141 gagccaacta tagtagacaa tgttaattgg gatgataata taatgaagga agaaatattt
     1201 gggcctatat ttcccatttt aacatataaa gatttggatg aagtaataca aaaaattata
     1261 caaatgccaa atccattggc actttatatt tttacaaaaa ataagtacct agaaaataaa
     1321 cttttagaaa tgattccagc aggaggatgt tgtatcaatg atacagttac tcatatagct
     1381 acgaattact taccttttgg tggtattggt gaaagtggta tgggctcata tcatggaaaa
     1441 gctggattcg atacatttac tcactataag agtgtgttaa aaaaattaaa tttggatgtc
     1501 cctattagat atgctccata cgataataaa ttaattaagg ttcttaaaaa gatgatgtag
     1561 gttatattgt agaataataa atataaataa aattgagtat ttgtattctg aatatatttt
     1621 atttttaatt tggtatattt tatttttaat ttggtatatt tgttataaaa gtggtgattt
     1681 tgtgataaaa tttaattata tagaaataag gaggaaaaaa tgaaaattaa ttggaataca
     1741 aaatacacaa caatagcaat ttatacattt ataatagctg ctagtagtat tatattttat
     1801 ctagtatcat cacaaattga tgtattttct aataatttag atgctatatt tacaacatta
     1861 caacctttta taataggatt tgctattgca tatcttctta attttatttt aaagttttat
     1921 gaagacagaa tttttataaa aagtgagaaa ttaaaaaaac taaaacaaag ttcaaaaaga
     1981 ggcttgggtc ttttattaac atatgcaaca gctacattga tactttactt atttatgcat
     2041 ttcgtattgc cacaagtaat tgagagtatt gttggactag caaatgatat acctatgtat
     2101 gtaaacaatg caactaagct tattgataaa ttaatgactg atttaaatct tgatgagcaa
     2161 tactttaatt tggcagttga taagtggaat gagtttgtta catatattat aaagtttgta
     2221 acagatttaa tcccaatatt aggaagtatg ttaaagaatg tagcctctag tatatggaat
     2281 attgtactag gattaattgt ttcagtatac ttattaattg ataaagaaaa attctatgga
     2341 ttaagcaaaa aaataactta tgcagtattt acagaaaaac aagctgctag aattttagaa
     2401 ttaactcata gaagtaacta tacatttgga agatttttag gtggcaaaat attagattca
     2461 ttcattatag gtattctaac atttgtaata cttacattag ttaaaatgcc atatacatta
     2521 ttaatatcag ttataattgg tattacaaat attatccctt tctttggacc tttatttgga
     2581 gctataccat ccacgctaat agtgatgttt gtatctccta taaaagcttt ctggttattg
     2641 ttaattatat taataataca acagatagat ggaaatatta taggacctaa aatacttgga
     2701 gattcaattg gtatatctgc attttggatt ttgttctcct tattggttgc aggaaaatta
     2761 ttaggtttta ttggaatggt tataggagtt ccaatgtttg cagtaattta ttctataatt
     2821 aaggatattg ttgaaagtaa attggacaaa aagggattac cgacagatac ttccgattat
     2881 atgtagctaa aaaatattat gtataattaa aaatatataa attaagcaat atttaaagca
     2941 ctgagaaagt taaactcttg gtgcttttta gatataaaat tattatagaa gaggtgtttt
     3001 ttgtggcatt tatagaatgt aaatttaaat ctaaatatac tggaggagaa actgatataa
     3061 ttgtaatttt gcctgtagca aatggaaaag aactttttag tggtaaagag atatatgatt
     3121 ttgaaggtaa atttaaaaca ttatacttat ttcatggttt agaagatgac caaagctcat
     3181 ggattagata tactaatgta gagcattatg ctaaaaataa aaatatagcc ctagtaatac
     3241 caagagtaga aactagtttt tatactgata tggcgtatgg acaccaatat tttacttttg
     3301 taagcgagga attacctaaa tttgttagag ctatatttcc tttatcagat aaaagggaag
     3361 ataactttgt agctggtatg tctatggggg gatatggagc tttaaaatta gcgctttcaa
     3421 aaccaaaaga gttttcggca gttgcaagtt tttctggttc gcctgatatg attcatgagt
     3481 tagaaaatca acaaatagaa accaatgcag acattctatt tcacgatttt ggtagaccag
     3541 aagacgctag aaatagtgag aatgatttac tttacatact taagaacctt aaagaaagaa
     3601 atgaagatat tcctaaaatt tatcaatttt gtggtgatag agatttttta tataaaaata
     3661 atcagatgtt taaaaaatat gcagattctc tgggtgtaga tgttgaatat gaagagtgtg
     3721 taggggggca tgaatggcgt ctttggaatt tatggataaa gaaatttata gatataattg
     3781 gatagtacac ttatggtatt aacaggtata ctataaaata tctttaaata tgataaagac
     3841 tgaaatccta aaatcaaatt atgattttaa aatttcagtc tctaagaatt taataaaatt
     3901 ttattaagtt cttacacgag ttattattgg agtaaaattg tcaactatat ctatatagta
     3961 tttactcaat tcctctaaaa tctcttcgca taaatccgta ctatgaccaa gattaataag
     4021 aaactgtttt gtaaaatgct ttattacagc ttgagaagat atatcgtact tgtgtgaagt
     4081 ataaactcta attatttcag gtttcatagc aagtatacca atacaagaaa gtattaattc
     4141 atcatgttct atagattctt tatcattaac atatcttttc atgaccattt cggttacact
     4201 tctttgatag cttaaaatag cttcttcata agctatatcc aaataaaaat ttctatatgc
     4261 ttcgtttttt agaaggaaac aaaggtcagc attcatagca agaaagaaca tcatcatagt
     4321 accttcttca tggtagttat agattaaaga ctgcatttca tttcttatat cttcatatat
     4381 tatagctcct atttcgcttt tacttgagaa gtaatagctt attagaccta aatttatatt
     4441 agctctttct gcaatttgcc tagttgttgt cttatgataa ccttgctcaa aaaataattc
     4501 tctagcagca cttataatac tagcttttgt ttctttgccc ttttctgatt ttgacatatt
     4561 tactgtgtat acctctcttt tcaatctttt tttgaaaatt tgctaactat aaatattgta
     4621 atagaaccac ctattatagg aaccaaaatt ctgtcaaata cagtaagaat accaatcata
     4681 tttaataatg ccaatataac acttactata acagcataga tagctccttt ttttgtagca
     4741 cctttccagc aaagaccaat tacaaatggt gcaaatatta cacctgtaca taatggagcc
     4801 ggccaaaaca ataattctaa taaactatca aatttaagtg ctatgattgc acctattata
     4861 gctgtcaaag ctgatgcaat ttgagaatac tttagagtat tttttaaatc attaaattct
     4921 atattaggat gtaataattt gttataaata tctcttgata taatagcact cattgatgtc
     4981 attaatgaat cggcagtaga cattatggca gccaatatag cagctaagaa taatacagat
     5041 attaaaggtg gcaaagtagt cgatatcatt tgaggtaaaa cctgaacaga tgatatgttt
     5101 ggaaataaac taccaccaat agctccaatt attcctatag ggaaagcaag aaataatagt
     5161 ataaatcctg caattacact acttttaact gcatttttag cattatcagc acattgatat
     5221 cttaaaaaac tttcataaga aaatattgag cctaaaacta atggtaaaac tgtatacata
     5281 aaatcactaa tcgatttatt tcctggaaag aatggttctt ttaacattga ttgtaacata
     5341 agtaaagggt tgtcgccaac taattttaag cttatataag ctactattgg caaaaacact
     5401 atacaaatag caagttgaat aaatgctgat actgcaactc cccacattcc tgatgtataa
     5461 gtatatataa tgataactat aactcctata attacagcta tatcataagg aataccaaat
     5521 gcagtagata cagaaccaaa agaaatcaat tgactaccca taatagctag tatagatatt
     5581 ggtgttacaa gactttgata agctcttaaa aatacactat gaccatatct agtttcaata
     5641 taatcagcag gtgttatatc acaagataag cttcttaatt ttcctgcgaa aaatgcattt
     5701 ataaagcaag ctaaagctgc acctatagta gaatacattc caacccaaag tcctctttca
     5761 gctccccatt gaactccacc aactaagcac cctcctccaa aagatgtagc agcaatggtt
     5821 gcagctagta taggcaaagg cagatttcta cctgcgatta ggaaatcgga tgctgtttta
     5881 actttttttg aaaaataaaa accaatccat atgtatattg cacacgtaac aattaaaagt
     5941 aacatagtct tttctcct
//
LOCUS       DACXMZ010000220         5851 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.221, whole genome shotgun sequence.
ACCESSION   DACXMZ010000220 DACXMZ010000000
VERSION     DACXMZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.221"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            231..1409
                     /locus_tag="JD016_16420"
     CDS             231..1409
                     /locus_tag="JD016_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAU5001204.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            1418..2074
                     /locus_tag="JD016_16425"
     CDS             1418..2074
                     /locus_tag="JD016_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HAU5001205.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADKIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            2074..2739
                     /locus_tag="JD016_16430"
     CDS             2074..2739
                     /locus_tag="JD016_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HAU5001206.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            2844..3620
                     /locus_tag="JD016_16435"
     CDS             2844..3620
                     /locus_tag="JD016_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HAU5001207.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGVTGQACLLDGGYTAQ"
     gene            3682..5016
                     /locus_tag="JD016_16440"
     CDS             3682..5016
                     /locus_tag="JD016_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HAU5001208.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGAGKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            5512..>5851
                     /locus_tag="JD016_16445"
     CDS             5512..>5851
                     /locus_tag="JD016_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HAU5001209.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PGNNKPESNKP"
BASE COUNT         2129 a          831 c         1019 g         1872 t
ORIGIN      
        1 ttttatccta taaatttttc ttatacacta tataaaaaat tagacttaga caaaacgctt
       61 ttttcgccgt aatctataat tagaaacagg aaaacctaaa gcattaggaa taaaagtaat
      121 tgaaatactt aaaaacaagc ttgtattaaa aaagtacttt attatagtgt tccctaattt
      181 ctttattcag aaaattttta attatcattc aagaaaggta actattagat atgaaagatg
      241 tagtaatcgt atcagcagtc agaacaccta taggaagttt tggaggtgta tttaaaaata
      301 cttcagctgt tcaacttgga actatcgctg ttaaagaagc gatatcacgt gtaagactta
      361 atttatctga aatagatgaa gtaataattg gaaatgttct tcaaacaggg cttggacaga
      421 atgttgctag acaaattgct ataaatgcag gtataccaaa ttctgttcca agctacacag
      481 taaataaatt atgtggttca gggcttaaaa gtgtccagct agctgcccaa tcaatcacat
      541 ctggtgaaaa tgatgtggta attgcaggtg gtactgaaaa tatgagccaa gctccttata
      601 tcgttccaac tgctcgcttt ggtagtaaaa tgggtgacat aaccatggta gatagtatgc
      661 taacagatgg ccttattgat gctttcaatc agtatcatat gggaataact gcagaaaata
      721 ttgcaactaa atttgaattt actagagaaa tgcaagataa attggcatta gaaagtcaaa
      781 ataaggctga gaatgctata aaaaacaacc gctttaaaga agaaatcgtt ccagtagatg
      841 ttttaatacg tcgaggaaaa atagaaacta ttgataaaga tgaatatcct aaattaggta
      901 tgacatttga aggtttagct aaacttaaac ctgcattcaa aaaagatggc actgtaacag
      961 ctggaaatgc ctcaggtatt aatgatggtg cagctatgtt aatactaatg agccaacaaa
     1021 aagcagacga actaggtatt agaccacttg ctaaaataaa atcctatgct tcagctggtg
     1081 ttgaacctga agtcatgggt actggtccaa tccctgcaac tagaaaagca ctgaaaaaag
     1141 ctggacttag catcaatgat atagacttaa tagaagcaaa cgaagcgttt gctgcccaag
     1201 cattagctgt taaaaatgaa cttcaaatag attcatcaaa gttaaatgtt aatggtggtg
     1261 caatagcatt aggacatcca ataggagcaa gtggagctag aatacttgtt accttaattt
     1321 atgaaatgca aaaacgtaaa gttgaaactg gacttgcaac actttgtatt ggtggtggtc
     1381 aaggaatatc aatggtggtt tcacgatagg aggaaatatg aataaaatag ttagcattga
     1441 tgaagctcta tctcatgtaa aagatggaga tacaataatg gttggtggat tcatggccaa
     1501 tggttcacca gaaaatttaa ttgattattt atgtagtaaa aatataaaag atttgacact
     1561 aatttgcaat gatactggct ttattgacaa aggagttggc aaaatggttg taaataagca
     1621 atttaaaaag ataattgctt ctcatgtagg attaaataaa gaaactggaa gacaaatgaa
     1681 caacaatgaa acagatgttg agttagttcc tcagggaact cttgctgaac aaatacgtgc
     1741 agctggttat ggtctaggag gtatactcac accgactggt attggtactc ttgttgagga
     1801 gggaaagcaa aaaattgttg tcgatagcaa agaatacctt cttgaaaaac caatttatgc
     1861 agatgttgct cttctatttg catcaaaggt cgataaagca ggtaatctag tttataaagg
     1921 ctctatgaac aattttaata atttaatggc atctgctgcc aaaattacaa tagttgaagc
     1981 agataaaatc gttgaaattg gtgatattga cccaaatgaa gttaatacac caggtatttt
     2041 tgtgaattac atcgttgaag gaggtaattt ataatggata aattagaaat gcaagaatat
     2101 attgcaaata gagtatctaa agaattaaaa gacggagccg ttgtaaacct tggaataggt
     2161 ctaccaacca aagtagcaaa ttatattccg gataatgtaa acgttatctt acaatctgaa
     2221 aatggctttt taggtttagg ttcagcagaa gatggaaatt caagtgatga aactattgta
     2281 aatgcaggtg gtcaaccagt tactatactt cctggaggat gtttctttga tagtgctact
     2341 tcttttggaa taatcagagg aggtcatgtt gatataactg ttcttggcgc acttcaggtt
     2401 gacaaatatg gcaatatagc aaactatatg attccaggta agatggttcc aggtatgggt
     2461 ggagctatgg acctagtaac tggagctaaa aaagttattg tagcaatgga acatacttca
     2521 aaaggttctg caaaaatatt aaataattgt actcttccac ttactgcaac aaatgctgta
     2581 gatttgatag ttactgaaat gggagttatg gaagtaacat cagatggaat tttacttaaa
     2641 gaaataaatc cagctttcac attagatgat gttatttctg ctactgaagc tcctttaata
     2701 ttgtcagatt cattaaatgg aaaaatatct gttgtttaaa ataattaaaa ctagaaaata
     2761 aaaatctctt agacgtatta catataaatt tgtattgctt aaaatattaa tataaaaata
     2821 aaataaaatt ggaggaaatg aaaatggtta aagataaagt tgtattcgta acaggagctg
     2881 caagtggaat aggtaaacaa atagctgaat cgttcttaaa aaatggtgct aaagtaatgt
     2941 tctctgatat aaatcaagaa gctttaaata ataccataac agagttacaa aaaaaaggat
     3001 atgattgcat gagtgtaaag tgtgatgtaa ctaaggaaga agaaattaac cacgctatag
     3061 acaaaactgt tgaaaagtat ggcagattag atatattaat aaacaatgct ggacttcaac
     3121 atgtttctat gatagaagat ttcccaactg ataaatttga atttatgata aaaataatgc
     3181 tgacagcacc ttttatagct actaaaaaag catttcctat aatgaaaaaa caaggctttg
     3241 gtcgtataat aaatatggcc tcaatcaatg gtgtaatagg atttgctgga aaggctgctt
     3301 ataactcagc aaaacatgga cttattggac taacaaaagt tgctgctctt gaagctgccg
     3361 attctggtat aacagttaat gctttatgcc ctggatatgt agatactcca cttgtcagag
     3421 gtcagttcaa tgacctttca aaaactcgta atataccatt ggaaaatgtc ttagaggaag
     3481 ttttataccc acttgtacca caaaaacgcc tattagatgt tcaagaaatt gccgactatg
     3541 ctatgttctt agcaagtgat aatgctaagg gtgttacagg acaagcttgc ttactagatg
     3601 gaggatatac tgcacaatag ttattatatt tagggcttga agaaagtatt cttcaggtcc
     3661 ttctactaag gagggatatt tatgagtatt atcgcgccaa ttggtataat tattggtatc
     3721 atagctatta tatatttttc agttaaagaa attcatataa ctatagctgc tccaatagca
     3781 actttaatag ttgttatttt aaataaaatg gatgttgtaa cgtcaatgct aggtgctggc
     3841 aaaaacaatt atatgggtgc acttggaaac tatataatga gttattttgc aatattctta
     3901 ttaggctcaa tacttgctaa atttatggaa gaaagtggag ctactgtatc aatagctgat
     3961 tttattttaa ataaatttgg ctctaatcat ccttatagag ttttagttgc tatatttata
     4021 gtaagtttta tattaacgta tggtggtata agcttatttg tagttatgtt tgcggtcatt
     4081 ccacttgcaa gaactctatt taaaaaactt gatatttctt ggaaccttat tcagatacca
     4141 ctttggcttg gtattgcaac aattactatg actataattc ctggaacacc agccattcaa
     4201 aatgttatac ctattcaata tctaaatacg tctcttactg cagctactat accaagtatt
     4261 gtaggtagta taggttgtgc tacgtttgga cttgtctata tgaaatatgc tctaaacaaa
     4321 agtattagaa aaggtgaaac ttatgcaact tatacttcag aattagaaga agaatcttta
     4381 gatagagaat taccaggatt ttttgcaagt atttttcctc tattagcact cgtaattata
     4441 gctataactg gaagtacttt tggttcagaa tttatgaaaa aaaatgtaat ttacatagct
     4501 ttaatagttg caattctttt atctgctttt ttattccata aatttctatc atctaagata
     4561 cagacattga gcataggtgc tagtggttct ataggtccta tatttgctac atcttctgca
     4621 gttgcatttg gagcagtaat aatggctgca cctggttttg atgtatttag caaacttata
     4681 atgtcaatac caggtagtcc acttataagt cttactgttt tgacttcaac aatgtcagca
     4741 ataacagggt cctcttctgg tgcattagga atagtaatgc caaactttgc agattattat
     4801 ttaagtactg gtctaaatcc agaattaatt caccgtgttg ctacaatagc ttcaaatata
     4861 ctaactatag ttccacaaag tggtgttttt cttacattct taagcttatc taagttaaca
     4921 cataaaactg gatttaaaga aactttcatt gtagttgcag ttggttcact aatagcagaa
     4981 attattgtaa ttacattagg attactaatg tattaatata aaacaaatta aaattcataa
     5041 tatgaaaggt ggtgtctcaa agtgaacttt ttacttaatg agacactctt tttatatgaa
     5101 ataacatatg cctatcattt aaacaataaa aaaggcctta tctctatttt gagacagacc
     5161 ctttttattt cttaaagatt taaataatta taatttgttg ctaatacgaa agcatctact
     5221 gaataaattt aaccaaataa aaaaaaattt atcataacta taaatttacg ttatggaatt
     5281 attataaaaa tattattttc tctaactaag taattccata ctatacaggc ttttaataca
     5341 aattgtcaaa cacactgaaa tatacctatt tattccatat atatcttttt tacatatctt
     5401 tacagtaaaa aaagtcttaa tcttatgtta gtatagattc acgggtaacc ccctacacta
     5461 aaggttacaa atcagtaaca tttttagaat ttttagatta agaggtgttt aatgatgaga
     5521 agaaatatac caacaaaatt aaaaactata ttagctttag gagtgttaac tttagcaaca
     5581 gcttcaagtg ctgttactgc aagtgcttca tcacttgaaa ataatgtaaa tataagtaac
     5641 aaaagtttag tatatagtaa tactaaatac aagttttctg caaaaccaaa ttgttctgta
     5701 aaaccagaac ttaagcctga taaagataac tcttgtaagg ataagaatca taatgacaag
     5761 aattgcaaca acactaataa acctgaggac aacaataatt cagactctac taataagcct
     5821 ggtaacaata aacctgagtc taataaacct g
//
LOCUS       DACXMZ010000221         5737 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.222, whole genome shotgun sequence.
ACCESSION   DACXMZ010000221 DACXMZ010000000
VERSION     DACXMZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.222"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(221..2053)
                     /locus_tag="JD016_16450"
     CDS             complement(221..2053)
                     /locus_tag="JD016_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001210.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2046..4292)
                     /locus_tag="JD016_16455"
     CDS             complement(2046..4292)
                     /locus_tag="JD016_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001211.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SIYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4360..4821)
                     /locus_tag="JD016_16460"
     CDS             complement(4360..4821)
                     /locus_tag="JD016_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAU5001212.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEKEFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(4994..5116)
                     /locus_tag="JD016_16465"
     CDS             complement(4994..5116)
                     /locus_tag="JD016_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001213.1"
                     /translation="MLYNQDTVEYDVVSKFILGQLFYLKIYEKVYFKLCNKKYK"
     gene            complement(5126..5692)
                     /locus_tag="JD016_16470"
     CDS             complement(5126..5692)
                     /locus_tag="JD016_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HAU5001214.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1820 a         1071 c          628 g         2218 t
ORIGIN      
        1 tttccttact acatattata ataaaataaa cactttgtaa agacataaaa ttatgtggct
       61 atctcaaaat atagataatc ctctttttca ttgttaaaat gataaaaata cactttattt
      121 catgcacaaa agagtgcctt atgaattaaa aagttcattt taaggcaccc tcatttattt
      181 tatcttatta attacatgtc tatgctaaga tagttttttg ttacattgct tcatcttctt
      241 caaattgaga attgtaaaga ttggcataga atccgccttt ctttagaagt tcctcatgat
      301 ttccttgttc aactatatct ccttcattca ttacaagaat catatcagca tcacgtatag
      361 ttgataatct gtgggctata acaaaacttg ttctaccttc cataagatta tccatagctt
      421 tttgaatcaa tacttctgtt ctagtatcga cagaacttgt agcttcatct aatattaaaa
      481 ttttaggatc agctaaaata gcacgagcta tagtcaataa ttgtttttga ccttgagaaa
      541 cattacttgc ttcttcattt aatttcatct tatatccatc tggtaatgtc ttaataaatc
      601 tgtgaacatg tgcagattta gcagcctcta taacttcttc atctgtagca ttcaacttac
      661 catatcttat attttccata attgaaccac taaataacca tgtatcttga agaaccatac
      721 caaacatttc tctaagctca ctacgattaa aatctttaat attatgacca tcaatcagta
      781 ttgaaccgct attaacatca taaaatctca ttaacagttt aactattgta gttttacctg
      841 ctcctgtagg tccaacaata gcaacttttt gaccaggttt aacatttact gagaaatcat
      901 ttattatagt cttgtttggg ttatatccaa agttgacatt ttcaaaatca attttacctt
      961 ctaaaccatc tattgaaact gcattttcaa caatttggac ttcttcttcc tcttctaaaa
     1021 actcaaatac tctttcagaa gctgctgctg ttgattgcaa taaattggca acctgtgcaa
     1081 tctgagtcat tggctgtgta aagtttctta catattgtat aaatgactgt atgtcaccaa
     1141 cctcaattgt tttcttaatt gcaagccaac ctcctaagat tgataccatt acatatccca
     1201 agtttccaat aaacatcata agtggttgca ttatacctga taaaaattga gatttccacg
     1261 ctgaattata caggctatca tttagtttat caaactcttc aattacttca tcctcacgat
     1321 taaatgcttt tactattgtt tggccactgt aagtttcctc aacttgacca tttacatttc
     1381 ctaaatattc ttgttgatat ctaaagtatt tttgtgattt ctttattatt cttgaaatta
     1441 caaacattga tattggtaat attaaaactg ccactaatgt cataattcca cttatagata
     1501 acatcattat caaaacacct atcatagttg ttactgatgt tataagctgt gtcatacttt
     1561 gatttaaact ttgatttaaa gtatctatat cattcgtaat tctcgataat atttcaccat
     1621 gcgtcttagt atcaaaatac ttcattggca atctatccaa ttttgctgaa atctcttttc
     1681 ttaaattata ggatactttt tgagaaattc cactcattat aaatccttgt ataaaagaga
     1741 acagtgcact tatcaaataa aggaaaagta aaaaggttaa gatttttcca attgcattaa
     1801 aatctatacc tacgtttcct ccagaaactt tactcactaa cccttcaaat atctttgtag
     1861 ttgcctttcc taaaattttt ggacctacta tagaaaacga tgcactacca atagcaaata
     1921 caattactaa aattattgat attttaaatt tagataaata tgcaattagt tttcgcatag
     1981 tccctttaaa gtcatttgct ttttgtcctg ctcccataga ttttcccata ggacctcttc
     2041 ccattctact cattagctag ctcctccttt gaaagttgtg ataaagctat ttgctcataa
     2101 acttcgcaat tttttagaag ctctttatgt gttcctattc caactatttc gccctcattt
     2161 aaaacaatta tctgttctgc atttaagata gtactaattc tttgtgcaac tattagaact
     2221 gtactttcag cagtttttga ttttaaagcc ttacgaagag ctgcatcagt ttttaagtca
     2281 agtgctgaaa aactatcatc aaatatataa acttctggat tttttgctat tgctcttgca
     2341 atagaaagtc tttgtttttg acctccagat acatttgttc caccttgaga aatttcagtt
     2401 ttaaaagctt ccggtttaga atctataaat tctgtagctt gagctatttc agctgctgtt
     2461 ctcatttctt catctgttgc gtcttttcta ccatatttta agttggattc tatagtacca
     2521 gaaaacaata ctcccttttg aggtacatat ccaatttttt cccttaaatc atgttgagaa
     2581 acatttttta tattaactcc accaagtaat atttctcctt cagtaacatc aaagaaacga
     2641 ggaattaaat ttataagagt tgacttacca ctaccagtac ttcctataaa ggcagttgtt
     2701 tctcctggct ttgctgtaaa agatatattt gttaatatag gttcttctgc gtctggatac
     2761 atgaacgaaa catttttaaa ttcaacaaga ccctttttat cttccttaaa ctcttgtgcc
     2821 tctgattttt ctgggtcttt tatgcttata tctgacacta atacttcatc tatacgtgta
     2881 gctgaaacgg atgctcttgg aagcataata gataccattg atatcattaa gaatgacatt
     2941 attatttgca ttgtatattg tataaatgcc atcatatctc caacttgcat tgctccagaa
     3001 tcaacactgt atgcacctct ccaaactata agaactgtga taaggttcat tataagcatc
     3061 atagcaggca tcatacatgt cataactcta tttacaaaaa gatttgtttt agttaaattt
     3121 ttatttgctt tatcaaatct ttcttcttca tgtttttcag tactaaaagc tctgataact
     3181 ggaataccag taattatttc acggcttact aagtttaact tatcaacaag cttttgaacc
     3241 agtttaaact taggcataac cactgagaat aacactacaa ttaatgataa gatagcaact
     3301 actgctactg ctataatcca ggccattgaa gtatttgtgt ttaaaacctt tatcactcca
     3361 cctatagcca ttataggtgc atagaatact attctaagca acataaccat caacatttgt
     3421 atctgttgaa tatcatttgt actacgagta attaatgaag ctgtagaaaa ttcattcatt
     3481 tcagtatttg aaaatgacat aacttttcta aatactccac ttcttaaatt tctacctaca
     3541 cttgcggcta ctcttgctgc taaaaatcct actgttattg tagctaccat actaatcatt
     3601 gctataccaa gcattttagc accagtaaat aatatataat tattttgagt tttttctgta
     3661 tcaataccta ttttctcata ttctgacctt acatatgaaa cagagctttg tgttatcata
     3721 ctctctggca tactttcaaa gcctttgctc atctctttag tcaatgtatc taactgttcc
     3781 tttggcattg ctgataacaa tttaaatata tcaacatctc cactttgagt agcttgtggt
     3841 ggaagtttac tcattaactg agctttcata gcttttacct ctttactatc gccctcaagc
     3901 cctgatacta ttaaaatagc tttaccaaaa ataacattta aatcatctat agtgtctttg
     3961 tctttagtat ttaactcata aatagattca tcttttagac ctgggtactt atctttatac
     4021 ttaacataat cctttttatt cagcaaagta taattatcta gtactttatc cttactactt
     4081 ttatccataa ataaagtaat tttatttaat tcactctctc taatcacaga tggaacagag
     4141 ttttctactc caccttgttg tatacctaca tttactatat tggatgtgta ttctggtaaa
     4201 gaaaggtcac ataccgcttg aattaccaat agacagacta ttattactat tgataatgcc
     4261 gatttcttta aatatttaat taatttaaac atacatttct tcccttctgt aatattaatt
     4321 agtctaataa cattaaattg aaaaacatta ttttaaactt taaaaatatt tttcatcttc
     4381 atgcttatga catcctgctt ttaaattatc tcgtacttgc attagaagtc ttctaagtac
     4441 tattttttct tcttcactaa atcctaaaaa aaactctttt tccaattcct tgttaaattc
     4501 ttcaacttta ttacatattt ctaaaccttt gtctgtcaaa aaaatcctag atactctttg
     4561 gtccttttca tcttgttttc tttccacaag tccagctttt tccatcctct ttatcataac
     4621 tgtagttgta gatgctttta ctttcctttt atctgctaat tctttttgac tttgcccatc
     4681 tttcatcttt aaagaaaaaa gtatttgggg ttgaccatga tataagccta atttatcaaa
     4741 ataaaaatga gttcttataa aatatattcg cattaattgc aagaaaacat catttaattt
     4801 ttcttcagaa ttgctattca tacttgccct ccttattttt tatattactt agtcagctaa
     4861 gtattatttt actatactgc atgcattttt tcaatatatt tataaaaaat tcatataaat
     4921 ttcatgtatt aaatattttt atatcatata gttataattt taataaataa tagtaagatt
     4981 tttaattagt cagctactta tattttttat tgcaaagctt aaaataaacc ttttcatata
     5041 ttttcaaata aaaaagttgt cccaaaataa attttgaaac aacatcatat tcaacagtat
     5101 cttgattata taacattatg atactctact tatcttcata tccatttaca acaacatcta
     5161 acttcccagt ttcaggtgat ataattagac catgtacaat aataccatct ggaattaaag
     5221 gatgcccttt tattaaatca atactatctt ttacagattc ttctgcagaa tcgaatccat
     5281 gtaaccattg ttttgtatca attccagcac tacgaagtgt aagtattaca tcattagata
     5341 ttcctctatc taaaatttta ccaagtaact tatccgtatc aacattacac ataccacatc
     5401 catgatgccc tacaaccata acctcatcta catcaaattc gtaaattgca actaaaacac
     5461 ttctcataat acttccaaaa ggatgcatta tagtagctcc tgcatttttt attagtttca
     5521 catctccatt tttcaaattc atagccttag gcaaaagctc agttaatctt gtatccatac
     5581 aagataagac tactattttt ttatttggat gttttgatgt cacatattgt tcatattctt
     5641 tgttctctac aaatgattta ttatattcta atatctcttc aagttttctc atatgtaata
     5701 tcaccctctt taaaaatttt tttattatta tatcact
//
LOCUS       DACXMZ010000222         5717 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.223, whole genome shotgun sequence.
ACCESSION   DACXMZ010000222 DACXMZ010000000
VERSION     DACXMZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.223"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..475
                     /locus_tag="JD016_16475"
     CDS             <1..475
                     /locus_tag="JD016_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAU5001215.1"
                     /translation="KQLINVDHQLLKIADVGYYVETPKTKSGNRVIPMSEKVLEAFQR
                     VLNKRKYAQPVILEGYTKFLFLNRNGLPKVAVNYESMFRGLVRKYNKTQKVALPKVMT
                     PHTLRHTFCTTLANAGMNPKALQYIMGHSNINMTLNYYAHTTSETSITEMKRLIA"
     gene            complement(775..975)
                     /locus_tag="JD016_16480"
     CDS             complement(775..975)
                     /locus_tag="JD016_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5001216.1"
                     /translation="MKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNPSLKL
                     AMDIAKVFDTTVENLFEFIEEE"
     gene            complement(988..1404)
                     /locus_tag="JD016_16485"
     CDS             complement(988..1404)
                     /locus_tag="JD016_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001217.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(1472..1885)
                     /locus_tag="JD016_16490"
     CDS             complement(1472..1885)
                     /locus_tag="JD016_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001218.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            complement(2701..3330)
                     /locus_tag="JD016_16495"
     CDS             complement(2701..3330)
                     /locus_tag="JD016_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAU5001219.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(3409..4371)
                     /locus_tag="JD016_16500"
     CDS             complement(3409..4371)
                     /locus_tag="JD016_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HAU5001220.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLIALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRSEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(5133..>5717)
                     /locus_tag="JD016_16505"
     CDS             complement(5133..>5717)
                     /locus_tag="JD016_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001221.1"
                     /translation="DSGNGGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPDPDN
                     GGNSKPDPDNGGNSKPDIDTDGDGKPNINIDTDGDGKPDINIDTDGDGKPDINIDTDG
                     DGKPDVNIDIDGDGKPDINIDTDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQNI
                     QTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT         1976 a          943 c          632 g         2166 t
ORIGIN      
        1 caaacagctt atcaatgtag accaccaact tttgaagatt gcagacgtgg gctactatgt
       61 ggaaacgcct aagacgaaaa gcggcaaccg ggttattcct atgagtgaaa aagtgttaga
      121 agcatttcag cgggtgctga acaagcggaa atatgcacag cctgttattt tagagggcta
      181 cacaaagttt ctgttcctta accggaacgg tttgccaaaa gtggcagtca actatgaaag
      241 tatgttccgg gggcttgtaa ggaaatacaa caaaacgcaa aaggtagcat tgccaaaggt
      301 aatgacaccg cacaccttac gccacacatt ctgcaccacg ttagcaaatg caggaatgaa
      361 cccaaaggca ttgcagtaca tcatgggaca ttccaacatc aatatgacgc tgaactatta
      421 cgcgcatacg acttccgaaa cgtcgataac agaaatgaag cggctgattg cctaagtagt
      481 aaacggagaa tttactactc cacatactac ttttgaacac caaaacatac ggggatataa
      541 gaagatttga gagtatcttc cacaatgaaa aatgccggaa aagcctgtaa caataggcta
      601 taccggcata taagaacttt tgaaaagata atcaaaaatt actttgtgat ttttaataaa
      661 acaattataa tctgacctta atcacatgtt atattatata aatattttta acaaacttaa
      721 taattaaata actatatttt attatccatc ttataaatgt ttataaattt tccactattc
      781 ttcttcaata aattcaaaca aattttctac tgtagtatcg aatactttag cgatatccat
      841 agctaatttc aaagaaggat tatatttccc attttctaag tgtacaatag tctcacgtct
      901 taccccaacc aatttagcaa gttcattttg cgtcataagg agtttttctc tgtattcttt
      961 aatttttgtt ttcatcaata ttgccactta gttacatatc ccccttttat caaagtatat
     1021 atacaacact aatttagata aagactgtat aaataaaact gcaatgagga ttatacctgt
     1081 atctaggttt gatatattta tattacttgt ataaggtgtt gcaaagaaaa tagatattat
     1141 tactatagaa tatatcatta tttttattgc aatcttatta acctttgata gtataaattc
     1201 agatgattca tcaactaaat cattatattt tttattataa attgaataac atccaaaaac
     1261 tattaaccaa ctcaaccctg gaagagaagc agtgcttgga cctttaaaaa tatacttagt
     1321 ggctaataca gctataaaaa aataagttat tacaagcaac tccaggataa gctttgatct
     1381 tacaccagtt tctgatttag acatataaaa tcactcctta aaaattatat tctctatact
     1441 ctttaatttt cgtttttatc aatattatca cttagttgca tattcccttt ctatcaaaat
     1501 gtatatacaa aattaattta gataaagatt gtataaataa aatagctaag agaattacac
     1561 ctatatccaa atttgatata tccaaacctc ttgtataatt tgctgaaaag aaaacagcta
     1621 ttagcaaaat agaatataac attatttttg atgcaatatg atcaaccttt aataacataa
     1681 gttttgacgc ctcgtcaact aaatcattat attttttatt atacatgaag tcacatgccc
     1741 aaaaagcgaa taaaattata aaagtaatac aaagatttcc ctctttaaaa ataaactttt
     1801 ttaataatga gtctataaaa aaataggata tcataagtaa ctctgaaaca agatttgttt
     1861 taacaccaag ctctgattta gacatataaa atcacccctt aaaagttata tatttataac
     1921 accttatgtt ataaatatat aacttttcca aataaattgc aatagaatat tgaaaatatt
     1981 caataatatc ttcataaata tattttataa aagattttat aataaacatt acaatacagt
     2041 actaaattag tttatttcat ctatcatctc aatcatatca tttactaact attattctaa
     2101 tgagcatata acattgaaat ttataattat acaacctaaa tgcctcccaa tgtaccctca
     2161 aaaacatcat ttcctgtaac tgaagtataa ttggtatatt ttatcactga aataaaaaaa
     2221 cactagtcaa taaagactag tattttcaag tattttatca ttcttttttt aattattgga
     2281 gacgaatcat cagaataatg cccataattt atctatatgc aaattctaaa tttaaaataa
     2341 agtttcagat aaatttacta ttctatgcta tgatacaaca gaaaaaactg aaatctatga
     2401 gtggtaactt catataatcc tagttcctac agtaatcaaa actgaggttt attctatatg
     2461 taactaatga taaattttat tataggagtt attagctcta tttgtgttac tataagtagt
     2521 aattatatat atgataattt tttaacactt ttctattgct atatagaaag tggtttagaa
     2581 tttaaattca ccaaaaagaa atctatatta gaaaccacta ctctattcca gcagattgta
     2641 attttttgtt ctttatatca caatgctata ccatattaaa ttgaaaaata aattttaaat
     2701 ttatttgtta caattcatta attcaaaaaa atattttgag tcatgagttt tgtaaagatt
     2761 tagccatgaa tttaatgctt ctgattcaaa aacatctaaa agtttcaata cttgaaacgt
     2821 aaattccaac ggagcaattc ctgaagccgt aactaaattt tcatcactaa ctacagattc
     2881 cgttttataa tatttttctc cagagtaatt tggtattacc attttaagaa actctaagtc
     2941 attacttgta tgatttctaa aatctaatag ccctttttta gctaagccta atgtagctcc
     3001 acatatggct gcaactatag aatcttgtat taaagcttct ttaacttttt ctaatattat
     3061 ctcatgattc atagataacc aagtatttcc ccctggcaaa attaataaat ccttactttc
     3121 catattaaac tcatcaactg taatttcagg taaaactttt aacccaccca ttgttataat
     3181 aggattttta tcaattccta cagtaattac ttttagtggc tttaatcctt ttttaaaata
     3241 tcttcctgta tttaattcag cagttaaata tgccacttcc caatctgaca tagtatcaaa
     3301 tacatacata tatacttttc tatttttcat cttttttact ccttatgtaa ttaatttata
     3361 tatctaatta taaaataact taactgacat attcagtcag ttaagctatt aaatttgata
     3421 ataatcaatt aattctagta aatcttcaat tattttcttc ctaaggctta gtggttcaat
     3481 tacttgtatt gtctttctat aaggtaaaag tatatttggt atgtacttgc atattacttc
     3541 ctcttcaagc ataaatatta ttttattagg tgttctttct ttcaagtagt gccctaaaaa
     3601 ccaatgttgg cttaaactat ctaaagaatc tatactccct tctacaacta attgaattat
     3661 attttgttca tcttttattt caggtataat tccttttata aaaaattcac ttgctgaaaa
     3721 ttctgtcggt ctgttaaatg tatttgatgt ttctatgatt tcactaatcc tatccactct
     3781 gaaactacgt acctcacttc tcatattaca aaatgcaact acgtaccaat tattattcca
     3841 gtgaacaatt ccatatggat ttataattct atattttgat tttgctttac gacctgcatg
     3901 atagttgatt tctacagata gctcctttaa tatactattc tccaattttt taagtactga
     3961 ttctatagac actttcccaa ttggttttat tacttcaagg gctattaagt ggtttgttaa
     4021 aatatcttct tgtttttgat ttgaatacat ttttaacttt aatatcgatt tatctaatgc
     4081 ttcgtctaag aagtatcctt cttttttagc aaatagtgct gcatgaagta gagatttttg
     4141 ctcttctata tcaaaaaaaa gaggtgcttt agtaaaatta tttaaaaggc tatacccacc
     4201 attatgacct gtatctgaaa ttataggtac tccactcgca gaaagtacat caatatatcg
     4261 gtacactgtt cttatattta tttctaattt ttcagctatc tgttttgctg taattttttt
     4321 gtcagagttt aacatccaca gtatagacat catattgtca attttagcca tgtagtttca
     4381 cctctttcta tttgttatat aaagttatta tataactttt tgattgattt tttacaaaaa
     4441 aacattagta aactgctaat aatttatact aagatttatt tcaaatattt atagatagtc
     4501 tttttctata gttaagttat ccattaaatt acatagtagt atatccattt tagttgatat
     4561 attttcagct acctcccttg aatattatta caataagtct aaatcttctt tcttccataa
     4621 atatattatg aggaaagact atcatcttta atggtttaat agtataaaag atattaattt
     4681 aaaaaaatct atttttatat cagatgggta ttgtttttta taaaactgaa aaaatcacct
     4741 aatagaatct atgatttttt aacataacct ccataaatta gtaccaatta tcacttcaaa
     4801 ttcaaatttt tatctgaaga atatacaatc atatttccat actcaacata atattgagtg
     4861 tttggcttgt atttacaaat tattattcta acaaaaacaa ctatacatag attaaaatca
     4921 tcttctaaac ataactacta aaattgaatt aatgatatca attccattaa tctcttttaa
     4981 taaaaaattc tcttttctat atgaaaatta tttcaaaact tttaagttaa atacaaaaat
     5041 gactataaat agatttttat ttacacaaat ctatttatag tcactatagt ttatccaata
     5101 aaataataaa ataaatatgt acacaataaa tgttatttac gtcttaagta tttatttgtt
     5161 agatttttaa ttaaaaaaaa caagctaatg aacattaatg ctaaatctag cattatattg
     5221 ctttgatctc ctgtttgtat attttgacct acattactat ctaaactttc atctgtatct
     5281 atatttatat ctggttttcc attcccatct gtatctatat ttatatccgg ctttccatcc
     5341 ccatctgtat ctatatttat atccggtttt ccatctccat ctatatctat atttacatcc
     5401 ggctttccat ctccatctgt atctatattt atatctggtt ttccatctcc atctgtatct
     5461 atatttatat ctggttttcc atctccatct gtgtctatat ttatattcgg ttttccatcc
     5521 ccatctgtat ctatatctgg tttgctattt cctccattat ctgggtctgg tttactattt
     5581 ccaccgttat ctggatccgg tttgctattt cctccattat ctggatctgg tttaccattg
     5641 ccaccgttat ctggatctgg tttgctattt cctccgttat ctggatccgg tttgctattt
     5701 cctccattac ctgaatc
//
LOCUS       DACXMZ010000223         5710 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.224, whole genome shotgun sequence.
ACCESSION   DACXMZ010000223 DACXMZ010000000
VERSION     DACXMZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.224"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            109..1434
                     /gene="cdeA"
                     /locus_tag="JD016_16510"
     CDS             109..1434
                     /gene="cdeA"
                     /locus_tag="JD016_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HAU5001222.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(1529..2323)
                     /locus_tag="JD016_16515"
     CDS             complement(1529..2323)
                     /locus_tag="JD016_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5001223.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMSLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2324..3199)
                     /locus_tag="JD016_16520"
     CDS             complement(2324..3199)
                     /locus_tag="JD016_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001224.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSESDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3203..3571)
                     /locus_tag="JD016_16525"
     CDS             complement(3203..3571)
                     /locus_tag="JD016_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU5001225.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3754..4413)
                     /gene="deoC"
                     /locus_tag="JD016_16530"
     CDS             complement(3754..4413)
                     /gene="deoC"
                     /locus_tag="JD016_16530"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAU5001226.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4449..5636)
                     /gene="murJ"
                     /locus_tag="JD016_16535"
     CDS             complement(4449..5636)
                     /gene="murJ"
                     /locus_tag="JD016_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAU5001227.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAKRKLH
                     LKK"
BASE COUNT         1905 a          899 c          631 g         2275 t
ORIGIN      
        1 attttttgtt gattttaagc tgtttatcgt ataaataaaa tttttcttga ctatatagtt
       61 actatatagc ttattatata tagagagtaa taaaaggagg taaaatttat ggaaaattta
      121 tttacaagaa aattcactac ttttgaattt ctaaaatttg tttctcctgc aattatatcc
      181 atgatattta tatctttgta cacaataata gatggcatct ttgtatcgac attagttggt
      241 tctgatgctc ttgctagtat aaatattgta ctacctataa ttaaccttgt ttgtggattt
      301 ggaataatga tggcaactgg tggaggtgct atcgtttcta tacgtatggg tgaaaataga
      361 caggatgaag ccaactctac attttctttt atagttttgt tttcattgat tgttgggatt
      421 ttattcacag taatctcata tttctttatc aaagaaatat ctattttgct tggtgcaaca
      481 gataagttat taccatattg tataacttat ggtaaagtta tgattttatg tacaccattt
      541 tatattttaa aatttatatt tgagtacttt gcaagaactg atggaaattc taaatttagt
      601 ttatttctat cagtcattgg tggtgtaaca aatataatat tggattatgt atttattaaa
      661 tattttggaa tgggtctttt aggagctgca gttgcaactg ctataggtat tattttaact
      721 tgtgttttag gtattattta cttcttatct aataaatcta cactaaaatt aagaaaacca
      781 aaaaccgatt ttagacttat aagagatact atgataaacg gttcttctga aatggttaca
      841 gaattatcta caggaattac aacattctta tttaatgtag tagctttaaa attagcagga
      901 gaaaatggac ttgctgctct tactatagta ttgtatgctc attttttaat gacatcagtc
      961 tatctaggat tcgctgctgg agtgtctcca ttaataagct ataattttgg tgctgaaaac
     1021 agtgataaat taaaagaaac atttaaacat tctctaaaat ttatatttgt ttcttctctt
     1081 ttagtgttta ttattgcttt agtatttgca ccatttattg ttagggtctt tgtaagtcca
     1141 gataacacag tatttaaact agccctacaa ggattaaaaa tatttgcatt tgcttttttg
     1201 tttgttggta taaatatatt tgcatcagga ttttttacag catttcacaa tggaaaaatt
     1261 tcagctatta tatcttttag tcgtgccttt gtttttataa tcataggaat cataattctt
     1321 cctcctatgt taaacatgac tggattatgg cttacagttc catttgctga ggttataacc
     1381 atatttatat ctattctatt tataaaaaaa tataaatgta gatataagta ttaaaaatta
     1441 atacttaaaa tcaacattta ttaaaactaa taaacctgct ttaaaataca aatttacaaa
     1501 tttgattttt aagcaggttt tattttagtt ataataagtc atttgcatac tctttaattg
     1561 gtgcttttct aagtaataaa attataaata ttatgcaaat aactataatt cctacttttg
     1621 ttccaaataa aataaaagga ttctgaaatg aataaataat tttttctaaa tacaatagca
     1681 ttttatgcat atttttagtt gcaaaattaa ctatataaat taccattaat atcggtaatc
     1741 ctatcaatat agtaatacta gtaacttttc taactctata tgtaaaagct ccaagtaaca
     1801 ctcctataaa acctatacat atttcattta tacatatttg tacaaaataa tttaaataag
     1861 ttagtgacat acttgatgtt gaaggatcta tcagtgctga tatagatgtc caaatcatat
     1921 ctctctttag taaaactgat agttctatac tataacctgt tataatttct ataaataact
     1981 tcgataaaaa aatcaaaact atacctaaaa tagaaatcac aaagcttaaa ataattaaat
     2041 ctatgacaat agcttttata caactttttc tatctgctct tatacttaaa gctcctgaaa
     2101 agtctttatt tgctatattt actccataca caaatagcag tatagttata tatgttaaaa
     2161 taggattcac aatcgtggat aaattagttt tagatgcttc tccaaaaatc ttgagaataa
     2221 acactggcaa aatattcata acaattccca caatcataaa tataattata tacaatttag
     2281 tatttttatt aaaaaatttt atatatggct ttaattcatt caattacaat tcctccttct
     2341 tagtcatgtt aacaaacata tcttgtagcc ctatgtatcc tacatcaata tttgaatttt
     2401 taagtatttt ttcatcactt tcactaaaat caccatagta aaaataagca actgcatttc
     2461 caaatgcctt tttaggtttt atatttttta tacattctaa tttttctaac tcttctttat
     2521 ttccagtaat ataatgagct ttttgtttta tgacatcaat ttcttcatct ataataattt
     2581 ttccctcatt taatattatt acatgctcca gtaaatcatc tatttcatca atcaaatgag
     2641 ttgatattat tattgtcctt ggatttttta tataactatc taaaattatg ttataaaact
     2701 cttgtctatt tactgcatcc aaaccaattg ttggctcatc aaatatggtg atagctgaat
     2761 tagaacaaat acctattata tttgaaagta tagttttcat acctcttgac agttgtttat
     2821 actttttttt agtatttaaa tcaaaatatt tgcataattc ttcttctaag cctttatcat
     2881 aacttttata aaaactagaa gaaaattcaa atatctgacc tactttaaaa tctggactat
     2941 aaaattcttt ttccctgaca atacaaactt cttccaaaac ttttatatct tcttttgggt
     3001 tctttcctaa tatttctatc tcaccttcat tttgaattaa ctgatttacc attatattta
     3061 ataatgttgt ttttccagct ccattcttac ctaataaacc atatatctta tttttttcaa
     3121 agtttagaga catattatta aaaatatttt gcttcttaaa actgtgagat aaattttcta
     3181 tcttaataga actactcata tattaatcct ccttgaaatt aattataata tcaaccaatt
     3241 cttctctatt tatttctaat ttgttagctt cttgaagtag gttttttaca aaattatgtt
     3301 taaaattttc ttttctagct tcttttataa tagtttttgc accatctgat acaaacatcc
     3361 ctatccccct cttcttatat aaaatatttt tatcaaccaa tatatttata ccttttaata
     3421 ctgtagctgg attaatttta taaagctttg aaagctctgt tgttgatgga acctgttctt
     3481 cttcttttat tccatttgat agtatctcat cttcaattgc tcgtgctatc tgaataaata
     3541 taggttcttc attatttaat actaatttca taggttcctc ctttctccat tagttagtta
     3601 ctcaactaat taactatata acttataata tcttgaaaaa tattttctgt caatactaaa
     3661 taaaaaacag agggaaaatt ccctctgttt tttgttcttt atatttataa ttaaatttta
     3721 atttttgtaa tcctatccca taagttaaaa actttaataa tctgttgtat cttcattttt
     3781 taatatttta catattgagc tagttcctaa cctttctgct cctgcctcta taaatttttc
     3841 tgcatcagaa attgatttaa ctccccctgc agcttttatt tttacatttt ttccaacatg
     3901 ctctttcata agctttatat cttctaaagt agctccacct gttcccattc cagtagatgt
     3961 ttttataaaa tctgaacctg acattgttac tatttcacac atttttattt tttcatcttc
     4021 atctaaataa catgtctcta taatgacttt taatacttta tctcctatag ctttttttat
     4081 ttcttttatt tcttttccta tattatcata atctttattt tttatatctg aaatatttac
     4141 aaccatatca acttcatctg ctccattttc aatagcatct tttgcttcaa atacttttgt
     4201 agcagtagtt tgatatccca aagggaaccc tatcactgta catatcttta ttttaccttt
     4261 tagatattct gatgctcttt tcacatatga tggtggtatg caaactgatg ctacactcat
     4321 atctacagct tcatcacata aaattttaat atcttcccat gtagttgtag cttttaaaat
     4381 agtatggtct actgttttta atatatgttt cataaaatcc tcctaaattt ataattaata
     4441 taattagatt acttttttaa atgcaatttt ctctttgcaa tgtttagtat catatcaacc
     4501 tcttcaactt taaaaataat cattagtagt gtataaattc cacctccaac tataactgaa
     4561 atagctagtg atacaaactc attaaatgtt cctactccca aaattccaaa tataaattta
     4621 tacgtaaagt atgataaaac acccataatt aaagatgcaa ctaccgattt caaagttgct
     4681 tttattattt tatcttgtcc aaaatatcca acttttctct ttaggttcaa gaagagtaac
     4741 aatatacatg caattgatga tgaactagtt gcaagagcaa gaccaccatg agccattggt
     4801 tttattaaaa ctaagtctaa cactatattt acccctacag atataatccc atttaccata
     4861 ggtgtttttg tgtcttgtaa tgaataaaaa acctttccca aaatatctct taagccaaat
     4921 gcagtcatac caactgcata aaaaattaag gcagttgctg tcatttgggt tgccctcgca
     4981 tcaaaagcac ctctctcaaa aatgattcta acaacaggag ttgcaaaaaa aatagaagca
     5041 actgaaatag gaatcataga aattattatc atatttatac tggatttaac tgaactagta
     5101 aacttctttt gattattttc tgcacttaac ttagataaca taggatacat aacagatact
     5161 attgaagctg taaatgtccc tgtaacaaaa ccatttaatc tatctgcata agtaagtgct
     5221 ggaatgcttc ctttaactaa tgttgaggct aaagttgtat ctaccaatga gttcacttga
     5281 ttaacagcaa cacctataaa tactggtgaa agtaatgcta gtaatttaat aaggctatca
     5341 tctttaaagt ttaagtagta taagtatttg tagttcgttt ttttaacaaa aaacatgtaa
     5401 aagagcagtt gaactaccat agcaaccaca gcaccaattg gaagtatgta tggtccaaat
     5461 atggtactta acattattga aataattatt actatattat agggtatact tccaaatcca
     5521 actacaataa agttctcttt aatttgtaaa aatgctgaca taacacttgt aatacctatg
     5581 aatattattc ctgttctgaa cctcccatga ctaaagtcac agggttcctg cttcatagaa
     5641 ttttgcatac taacatatgt cttactaatt ctccacaggc tatccccgta gttcctacgg
     5701 ttcttacata
//
LOCUS       DACXMZ010000224         5694 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.225, whole genome shotgun sequence.
ACCESSION   DACXMZ010000224 DACXMZ010000000
VERSION     DACXMZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.225"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..518)
                     /locus_tag="JD016_16540"
     CDS             complement(<1..518)
                     /locus_tag="JD016_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAU5001228.1"
                     /translation="MIVRIKFNPSFKEANFTKKRYRAMKGSAGSGKSVNVAQDYILKL
                     GDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIKSKVT
                     GNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGILTNP
                     NLYYQMTFTFNPV"
     gene            complement(508..1191)
                     /locus_tag="JD016_16545"
     CDS             complement(508..1191)
                     /locus_tag="JD016_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU5001229.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWASE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETRELIQEISIKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDELKIS
                     VDYGDRNDS"
     gene            complement(1321..1518)
                     /locus_tag="JD016_16550"
     CDS             complement(1321..1518)
                     /locus_tag="JD016_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001230.1"
                     /translation="MDDLRGKIIKELDEFNIDADDEFLDYGVEYVESFTGKSAISEDL
                     LIRGVVLMANYIVNMENKDLD"
     gene            complement(1986..2468)
                     /locus_tag="JD016_16555"
     CDS             complement(1986..2468)
                     /locus_tag="JD016_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001231.1"
                     /translation="MNKKTLFQEVEGRLYNYKKLESQIRIKDIYIKKLENEFCGCKAQ
                     SYEEKTGETYNINSSVENEVIKREEDLNRLKEDKKTLEIEKETIECALTSLNSFETEF
                     FNEMYMNNEKINMDYMSNAMHIDRSHCFRIRKRIVCKIMDMLYPKIKEFELPIFSWKA
                     "
     gene            complement(2572..3312)
                     /locus_tag="JD016_16560"
     CDS             complement(2572..3312)
                     /locus_tag="JD016_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAU5001232.1"
                     /translation="MNNLKLIENEGLIKVYTTDEDIKVVDGRELWEGLEVEKDFSDWI
                     KSNLENVDAAEGIDFSTLKGKTSEQGGRPAIEYILNLEIAKEICLVAGASPRANKELK
                     RNSKNYRKYLIAVEEKYKVSNNLTKTQLNQINDIVSNALCEMQTKHDAQLEQFKKESS
                     QYYRPTSKTKYDISSYIKDRLDIPRANEEFDLVKKRTLLILGADKWEDIPKDVLLKSL
                     NIIDESIRIIKSERKTKQISFFEKDNFC"
     gene            complement(3375..3782)
                     /locus_tag="JD016_16565"
     CDS             complement(3375..3782)
                     /locus_tag="JD016_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAU5001233.1"
                     /translation="MKVFLVIDGEPVGKERPRFNLATKRTYTPQKTKDYEELIKWLYQ
                     SKVRHYFTGYIKMTLRCYYSIAKSNSKKIKEQKRNNVLRPNKKPDIDNVVKIIADSLN
                     EIAYKDDTQIVEVVASKYYSDKPRVEVILEDIS"
     gene            complement(3779..3892)
                     /locus_tag="JD016_16570"
     CDS             complement(3779..3892)
                     /locus_tag="JD016_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001234.1"
                     /translation="MKRRRCSWCGKLFYLEEKSKDVYCCKECRKKAKKVKK"
     gene            complement(3908..4249)
                     /locus_tag="JD016_16575"
     CDS             complement(3908..4249)
                     /locus_tag="JD016_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HAU5001235.1"
                     /translation="MANIYCENYNCKNYFEDMCMLERIEINNFKECESYLEGKNELYK
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEVLEEELTK
                     INSHLELEDLR"
     gene            complement(4348..4677)
                     /locus_tag="JD016_16580"
     CDS             complement(4348..4677)
                     /locus_tag="JD016_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001236.1"
                     /translation="MKIFLLTILLIIICILANYVKNRIYKKSINNLKYKYSVGEKIIY
                     HQINCYYNRMVGCEILEKCYSTKFRKRNTPLYKVKAYVGDNNTTWVIPEWRIECLATT
                     YGEFPKY"
     gene            complement(4759..>5694)
                     /locus_tag="JD016_16585"
     CDS             complement(4759..>5694)
                     /locus_tag="JD016_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAU5001237.1"
                     /translation="EGRVSQDIEAFFQKKISKVSRDNDIRIAVFNDYSNNLIKSCCEQ
                     IIYDESSFLNNSKEIASYLFEIMKLNATLESCDLAICLYSQKDEKKVAILKLDYNKSY
                     THSIEFKDDKFNIQMSKNEINIQETKTVKIAALVGLSGMNDKYHLRVLDKDAEKEEAN
                     SKFVTEFLNATKIKDDKYKTKKFKNTAENWITNALSNDIKQAEDVRSILNYTLREKHE
                     IDINDFVDKTIKDDKLKDSFKEHMEEKGLVEGFSIDKKWVDKKLKKRNIKTDNGFEIK
                     GNLTDFEDPMKYTVRQNQNGSIDIVIKNVTFYEEK"
BASE COUNT         1659 a         1057 c          572 g         2406 t
ORIGIN      
        1 actggattaa atgtaaatgt catttgatag tataggttag gattagttaa aatacctctt
       61 aaacggtcgt ctagtatgtc tatgtcactt tccataagtt ctgtagcttc ttcacaccaa
      121 acccatgtta attttccttt cgagaagtta attgatttta atttttctct ttgttttgca
      181 tcattaactc ctctgaaaat tatagagtta ccagtaactt tactcttaat ttctaaagga
      241 tttaaagtag ttttccaata cttatcagct tgtttaccat aaatacgatt tatagctcct
      301 gtaagctctg catacgttga atacttatgt gtagcttctg attttctaac tactaataag
      361 ttagctcctt gatacttttt atctcctaac tttagtatat aatcctgtgc cacattaaca
      421 gattttccac tacctgctga acctttcatt gctctgtatc tttttttagt gaagttagct
      481 tctttaaaag atggattaaa ttttattcta actatcattt ctatcaccat aatctacact
      541 tattttcaac tcatcatctc caatatcatc cttacttagg ttatcaacct cacattttaa
      601 cttctcaact ctagtttttt gctcctctgt agccaaattc caatccttat gaatcatttc
      661 atcatactgt ttaattaaac ttctaagttc actcatagct ctactctgtg cattaagaaa
      721 agatgcttgc ctatcccatg caaattgaaa ttcatactct atcttctcac cattttctgt
      781 gctttcatgt ttctttaatt ccttaatcat ttcttctttg tctttaacat acattatctt
      841 ctgtgctctt attattgctg catattgaat tgttatctgt tcccaaagaa tatcaaattt
      901 atcttttata gatatttctt gtatcaattc cctagtttct tcaggtagat attttgagaa
      961 gaaaccaaac ttttcagcat tcttattacc aggtggacca gtagcatttt tattacctat
     1021 gggtgcaccc cttttatttt taggtgcacc cttctttttt tcactagccc agttgtatct
     1081 tttaatccat gactttaaag tgtttaaact aatgtcatac tttgctgata tttccttttg
     1141 tttcatacct tttaggtaat cttgttttac cttttctttg acatcttgca catcaccacc
     1201 tcgtttgttt gtcgttttgg gaataaaaaa agacctagaa attaatctga gtcttttcta
     1261 ctgtcggagt taccgacatc taaaatttat taaatttttg tattaaaaaa gacctagaaa
     1321 ttaatctaag tctttatttt ccatatttac tatataattt gccataagca ctactcccct
     1381 tatcaataaa tcttcactaa tagcactttt ccctgtaaaa ctctcaacat attcaactcc
     1441 ataatctaaa aattcatcat ctgcatctat attaaattca tctaattctt ttattatctt
     1501 tcctcttaaa tcatccatat ttttattctc cttttttaaa aataaaagac taagcttgcc
     1561 cttgcttagc cttttataat agggaggtac atatattatg ttgcaagttc taagaatcga
     1621 acttagatta aacaccagcc cttgcatggt gagtgaggtt accaagcccc actcgattta
     1681 gacacctgaa ttaagataca aaactgtatg aattttagtc tcaattcata tactattttt
     1741 agtgtatccg tagattaatt taaatagaaa aaaacttaag gattgaacat agttagaatt
     1801 gaactaacag catcctcatg ccctgcctag tctgttcata taagctagag taatccctta
     1861 accctagcct aatatatatt tagttttgag agggaaatct ttatttccac gatattatta
     1921 tctcatgttt ttaccaataa aaagtctcac gatagtctcc aaaaagtctc aaaatagtct
     1981 catttttaag ctttccatga aaaaatgggt aattcaaact cttttatttt tggatataac
     2041 atatccataa tcttacaaac tatccttttt cttattctaa agcaatgact tctatctatg
     2101 tgcatagcat tagacatata atccatgttg attttttcat tattcatata catttcattg
     2161 aagaactctg tttcaaagct atttagactt gtcaatgcac attctatagt ttctttttca
     2221 atctctaatg ttttcttatc ttcttttaat ctatttaaat cttcttctct tttaataact
     2281 tcattttcaa cacttgaatt tatattataa gtttctccag ttttttcttc ataactttga
     2341 gctttgcatc cacaaaattc gttttctaat tttttaatat atatatcttt tattcttatt
     2401 tgactttcta gttttttata gttatataat ctaccttcaa cctcttgaaa tagtgtcttt
     2461 ttattcatac ttccacactc ccatcaattt tttatgttat aataatattt gtatataaaa
     2521 gttttatatt tttgacaagt ggagtgtgaa agcacccctt ttttcttttt attaacagaa
     2581 attatctttt tcaaagaaac taatctgttt tgtctttctt tcagatttta taattcttat
     2641 tgattcatct ataatattta gggattttaa taacacatct tttggtatgt cttcccactt
     2701 atcagctcca agaattaata aagttctctt tttaactaaa tcaaactcct cattagctct
     2761 aggaatatct aatctatctt ttatgtagct tgaaatatca tacttagttt tgcttgtagg
     2821 tctataatat tgtgaggatt cttttttaaa ttgttctagc tgtgcatcat gcttagtttg
     2881 catttcacat aatgcgttac ttacaatatc attaatttga tttaactgtg tttttgtaag
     2941 attgtttgat actttgtatt tttcttctac tgcaattaga tattttctat aatttttaga
     3001 atttctttta agttctttat ttgctcttgg acttgctcct gctactaaac aaatttcttt
     3061 tgctatttct aaatttaata tatattctat tgcaggtctt ccgccttgtt cagaggtttt
     3121 cccctttaaa gtggaaaagt ctattccttc cgctgcatct acattttcta agttactttt
     3181 aatccagtca gaaaagtctt tttctacttc taacccttcc caaagttccc taccatctac
     3241 aacctttata tcttcatctg ttgtatatac ttttataagt ccttcattct cgattaattt
     3301 taaattattc atacttataa tcctcctcca aattattttt atctaatgat ttgataaacg
     3361 aatttttccg ttgttcaact aatatcttct aatataacct caactcttgg tttatcactg
     3421 taatatttac tagctacaac ctcaacaatc tgtgtatcat ctttataagc tatctcattg
     3481 agtgaatcag ctataatctt gaccacatta tcaatatctg gttttttatt gggtcttaac
     3541 acattatttc ttttctgctc tttaatcttt ttactgttac tttttgctat agagtaataa
     3601 catcttaaag tcatttttat gtatccagta aaataatgtc taactttaga ttgatataac
     3661 cattttatta attcttcata gtccttagtt ttctgtggtg tataggtcct tttagtagcc
     3721 aagttaaatc taggtctttc tttaccaact ggttctccat ctattacaag aaaaactttc
     3781 attttttcac cttcttagcc ttcttcctac attctttaca acaataaaca tccttagatt
     3841 tttcttcaag ataaaataac ttaccacacc aactgcatct tcttcgtttc ataagctcac
     3901 ttcctattta gcgtaaatct tctagctcta agtgagagtt tatttttgtt agttcttctt
     3961 ctagaacttc caaacactta tttttatttt ttaaaatact atttgtagaa cggcatttta
     4021 ctgtaatacc agttggaata tgagtaactt caacagaata atctttactt ttcaccattt
     4081 tcaaatcttt aggatgtata gtatatccgt tttctaattt atatagctca tttttacctt
     4141 caagatagct ttcgcattct ttgaagttat taatttcaat tctttcaagc atacacatat
     4201 cttcaaagta gtttttacaa ttataatttt cacaatatat attagccatt taacacactc
     4261 cttttataag tcaaagtaag tctataacat tctagtttca ttcataaact taccttgact
     4321 atttatactc caaccaattt ttattattta atatttagga aattctccat aagtcgtagc
     4381 aagacattca attctccact ctggtataac ccacgttgta ttgttatcac ctacatatgc
     4441 ttttacttta taaagcgggg tatttctttt tctaaatttc gtactataac atttttctaa
     4501 aatttcacaa ccaaccattc tgttatagta acagtttatt tgatgatata taatcttttc
     4561 ccctacagaa tatttatatt ttaaattatt tatagatttt ttatatatgc gatttttcac
     4621 ataatttgct aatatacaaa taattattag cagtatagtc aataaaaaaa tcttcataag
     4681 ttattcctcc taactagttt aaatttacat cctcttatta tttacttctc cttctctagc
     4741 caatagtcac atgagtacct acttttcctc ataaaatgtt acattcttaa taactatatc
     4801 tatagaccca ttttgatttt gtcttactgt atatttcatt gggtcctcaa aatcagttaa
     4861 gttacctttt atttcaaagc cattgtcagt ttttatattt ctctttttaa gctttttatc
     4921 aacccatttt ttatctatac taaatccttc aacaagacct ttttcttcca tatgttcttt
     4981 aaaactatct tttaacttat catctttaat tgttttatca acaaaatcat ttatatcaat
     5041 ttcatgcttt tctctcaaag tataatttaa tatacttctt acatcctctg cttgttttat
     5101 atcattacta agagcattag ttatccaatt ctcagctgta tttttgaact tcttagtctt
     5161 atacttatca tcttttatct tagtggcatt taagaactct gtaacaaact tagaattagc
     5221 ttcttccttc tctgcatcct tgtctaaaac cctaagatga tatttgtcat tcattccact
     5281 caatccaacc aaagcagcaa ttttaaccgt cttagtctct tgtatattaa tttcattttt
     5341 agacatctgt atattaaatt tatcatcttt aaactcgatt gaatgagtat atgacttgtt
     5401 gtaatcaagc tttaatatag caactttctt ttcatctttt tgagagtata agcaaattgc
     5461 taagtcgcaa gattctaatg tagcattcaa cttcataatc tcaaacagat aagatgctat
     5521 ttctttagag ttatttaaaa atgaactttc atcatatata atctgttcgc aacaactctt
     5581 aattagattg ttactatagt cattaaatac agctattcta atgtcattat ctcttgatac
     5641 tttgcttatt tttttctgaa agaaagcttc aatatcttga ctgactctac cctc
//
LOCUS       DACXMZ010000225         5688 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.226, whole genome shotgun sequence.
ACCESSION   DACXMZ010000225 DACXMZ010000000
VERSION     DACXMZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.226"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            219..401
                     /locus_tag="JD016_16590"
     CDS             219..401
                     /locus_tag="JD016_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001238.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            complement(689..912)
                     /locus_tag="JD016_16595"
                     /pseudo
     CDS             complement(689..912)
                     /locus_tag="JD016_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431039.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
     gene            1233..2147
                     /locus_tag="JD016_16600"
     CDS             1233..2147
                     /locus_tag="JD016_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HAU5001239.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            2537..3184
                     /locus_tag="JD016_16605"
     CDS             2537..3184
                     /locus_tag="JD016_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HAU5001240.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(3351..3920)
                     /locus_tag="JD016_16610"
     CDS             complement(3351..3920)
                     /locus_tag="JD016_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAU5001241.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(3968..4825)
                     /locus_tag="JD016_16615"
     CDS             complement(3968..4825)
                     /locus_tag="JD016_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAU5001242.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFFKR"
     gene            complement(4836..4985)
                     /locus_tag="JD016_16620"
     CDS             complement(4836..4985)
                     /locus_tag="JD016_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001243.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(4978..5562)
                     /locus_tag="JD016_16625"
     CDS             complement(4978..5562)
                     /locus_tag="JD016_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAU5001244.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         2087 a          856 c          658 g         2087 t
ORIGIN      
        1 atatctacta actcaaatat tcataattaa actttaaact ggataaattt tcttttaaga
       61 atgaaagttt atccagtttt gcgattataa taaccagttt tacatatcca cttgtaaaat
      121 atatctaagc aatatatcat actatataga tttatgagtg aaataatatc tgctatttac
      181 tgaatatttt tatttttcgt tttttagggg gattagttgt gtttaaagat aagattgatg
      241 aatgtgttca tataatgact gcatatattg ctagtttaaa agaatactat tcattcatag
      301 aaactcaaat tggtgatttt attaaaaaat atggagaaga tgttgtagag ttatgtctcc
      361 atagagtgat gattctacta tgcgaatgtg gtttggcata aaattaaatt tattaaaatg
      421 ggtaagaaga atcctatata atattcttct tacccatttt aatatatcaa tttcatagct
      481 tatcgtttaa aatattttac atccattact agacaacttt acattctctt tttaattcta
      541 gtatatttaa atcattttag ttgctcctta tttattctta ttacttgttt tattttataa
      601 ataaaataat tctttttaat aatgtttttt gttgatattc ctatcacaca tccaattatt
      661 tatcattgtc ttgaaattgt ttgcatactt aatttaatcc aattgaaaca ccccagcata
      721 gaatcccctg taaaatagaa gttggattaa atcctaacat ctatacagaa aatactactc
      781 caagcacaag caatataatg gaatatattt atcacttaat tttttatact ttttaaatcc
      841 catacctaaa acattaagtg cagctattag aattaataat tgttgtggta taaaattatg
      901 cttgcttaat atttattcat tttttactat aaaagttctt gttttacaaa aaacatttgt
      961 tttgtatcaa aataatactt ataaattaca taattctcat aaaaatttct agtattactt
     1021 gtactcttaa taaataatag ccaacaagta ccttaaatca ttatatctat atctaataag
     1081 aaacacagta agataatttt ttcgcccata acttaataaa aactgttgat attagtagca
     1141 attgaattta gttaatttat ttataatata cataaattta aatattacga ataaagtatt
     1201 ataaatagga tactttctaa agggaggaaa ttatgataga taatcaaaaa tatgttattt
     1261 tatcactaga attacattta tttttttcaa gaattatgaa agagcatgct ctttttttag
     1321 aagcaggatt cacaaataaa aattataatc ttgctatgga agctgaccac tataaaaagc
     1381 aatttgaaga tttattatca tacactgtta gtgctagtaa tggtataatt agacctgata
     1441 tattatattc agaagagctt gtaactactc tcacatcagt tgcagaacaa aaaacagaag
     1501 agtttacagg gatagaaata aacaaaaaca tcactacaag agaattaaat ctacaaagtg
     1561 gtgtaaaccc acaagttggt caagatttag tgaactatgt agctcaactt aactctgatg
     1621 caataagatt acttgatggg cttattaatt ttaaagaaag agtcttagat ggtgtactat
     1681 catgtactat atttacatca aactaccctc tacttcttga acatataata catgaagcaa
     1741 atttatatcg ttcttatgta gttgaccttg aaaataaaat agatattgag tcaaaaaacg
     1801 ctaaagaaat agaattattc tgggaccata ttatgatgga acatgctctg tttatgagag
     1861 gattactaga cccctcagaa ggtgaactaa taaatacttc aaatgatttt gctataaaat
     1921 ttaatgaatt aattgaaaaa acaaacgaaa tgactgattc taatatcaag aacattacag
     1981 aagaaactct aaatgaaact gttgagttta aagattttaa agaagcagga gcatcaggaa
     2041 tagaacagtg taagataaaa tctataatat taccactttt agcagaccat gttttaagag
     2101 aggcaaatca ttatattaga atattggaga gttataaaaa catgtaatta aaaattactt
     2161 ttaaaataat ctttgcatat ttagataaaa taaaggtaca gttaactgta cctttatttt
     2221 ttataaactt ataaaattta aacaagttat tcataaaata aaataataat catctataac
     2281 ccacaacttt attttatatt aaataaaaag tattagtgat tatgttttat aggtatttct
     2341 ttattcagtt atttattaaa aactaatagt aaatcttata ctaatcgcat atttatgtta
     2401 taactatatt gtaactattt tcgtaatctt aggaaaaggt tacttataaa attaacctta
     2461 tacaataata tatcgtataa tcttaattag cataatagtt acaatattaa aatttaaata
     2521 tgggagtgat tttagtatga taagacaaac accattagag aaacgatatg acaagctagg
     2581 tcctcatatt gtaacagcac tcaaaaaacg ttattttgat gcttattact gtaaaactag
     2641 aaatgaagct ttgcaacaaa ttttagattt aattcctcaa aatcatgtag tatcatgggg
     2701 aggatctgaa acacttaaag aaatgggcgt acaaactgct gttagagaga aaggatttaa
     2761 agttattgat agagatttag caaaaactcc agaagaaaga actgaaatta tgcgtcaagc
     2821 acttctatgc gatacttttt tgatgagtag caatgcaatt agtgaagatg gacaactctt
     2881 taatattgat ggaaatggca atcgtgtagc tgcaatgata tttggtccaa aaaatgttat
     2941 agttgtagct ggtatgaata aaattgtaaa aaacattgat gatgcaatac aaagaactag
     3001 aacaattgca tctccagcag ttgttcagcg ttttcaagat gtcaatacac catgctatat
     3061 taatggaagt tgtatagact gcacaagtcc tgaaagcatt tgtgcataca tggttactac
     3121 aagaatcagt cgtccagcaa aaaaaataaa ggtaatattg gtaggagaaa atcttggtct
     3181 ataaaatata catacatgta aatagctcta taaataataa aatcatatga aataacttat
     3241 ttctaaaagt ggtagaataa aagcttctac aagactttca ttctaccact tttatattct
     3301 tttaatttgt gtttctatat tattaattgc tccgattttc atatatatta ctaactattt
     3361 ttattttcat aatttttttg ttcttcatca agctgtttat aataatcttt attatagtca
     3421 gctagataat ctataccacc tcctgtaatc acatttaata atttcattgc agattcttga
     3481 ttaactttac aaacataatt atcaccagat ttaacaagct ctaatgtaat attaaattct
     3541 tttttctttg cagaatcaat atctttcata gttggatttg gcatcatttc tcctagttta
     3601 ggaggattct tttttaaaat agaaaccata tcagtattgt taatttttaa atctacagtc
     3661 actttatcct tattattctt tatatctgaa atttcccaag tcatattttt agaatacatt
     3721 ttaaatcctt ctttatcttc atatataaga ttacctaacc ctgattttgg gaaatactta
     3781 tcaacagtat ctgcatcttg tgtttttatt gaattaaaca tactatccac ttcatttttt
     3841 atcttattat cattagtaca accagctaac accagtactg atattgcaaa cattataata
     3901 catataataa atttcttcat taaattatat ccccctatac ttttttgcac aaatattatt
     3961 ctctttttta tcttttgaaa aaagttgtag taattgcatt ttgtggacaa acttttatac
     4021 attcaccaca cctaatacag tctggactat ttacatcttt atatattggt atatcaagct
     4081 tacattttgt ttgacatgct gtacaatttg tacatttatc tttatcaatt ttaaatctat
     4141 atattgaaat aggattaaaa aaaccataga ttgctcctaa agggcatata tacttgcaaa
     4201 atggcctata agtaatgatt gacatcaata atatcaatag caaaatactt aacttccagc
     4261 taaataaaaa tcctattgat tctcttaaat tctcatttcc taaaataagt ggtattcctc
     4321 ccaaaagtgt tcctgaaggg caaatccatt tacaaaacca aggatttcca ccccctgcta
     4381 catttacaat agcaactggc aaaataatta caaacattat taagactata tactttagat
     4441 attttagata tttatcacca taaacttttt ttatctttac tggaaagggt actttatgta
     4501 gtaaatcttg tacaagtcca aagggacaaa gccaaccaca aacaaatctg ccaaaaaaag
     4561 caccaaatgc cattaaaaaa ccaacaacat aaaatgagaa tttatactct ctgcttgata
     4621 aaactgcttg taatgaacct attggacatg ctccaagtgc acctggacat gaataacaat
     4681 tcaatccagg tacacataat tttttacttt ctcctccata tatagttcct tctaaaaatc
     4741 catttacata tccatttgtt aatgctgtaa atgcaacttg agtaataagt cttatattat
     4801 tatttacttt agataataac ttcattcctc ttctcctaac caataccaat acactcaaga
     4861 caaatcataa ttgctttagt taatactgta tttgtttctc cacgatatgt acctaatgca
     4921 ataaatataa tactaattgc aataatagaa atgccttttt tattatcttt aataaaatta
     4981 ctcattggct tttaccattt ttagtctttc atctataatt tttttccaat cttccttact
     5041 atttgcacct acaaccttct cacccacaaa gtttcctttc ttgtctacaa aataagttgt
     5101 tggaaactcc ataactcttt taaaatcagt ttttttaagt tcttcagata cagtcaattg
     5161 ttggtaagtt gcatctgact ttttcataat gtctttaact aaatttttct cttcatttga
     5221 aagaccagtt ctcatatcag ttttatcaac ttctacaact aatcccttta tagctacatt
     5281 ggaattatta ctctcatatt cttttgataa tgcttcaagc tctggcattt cacccacaca
     5341 tggtccacac caagtagtcc agacattaat aagagtaagt tctttatttg aaaatatctc
     5401 ttttgttacc ttatttccat caacatcctc cgtatctaaa ttactaaacc attcactatt
     5461 attcttgttt ttttctactg gcttagattc ttttttgcta catccagaaa tcatacctaa
     5521 aacacaaata catcctaaaa ctactatgaa aaaccttttc atatctcaca ctcctagaat
     5581 ttatatagta tataaaatcg ctgcttgaaa taaaaataac ttatcttcta tattaaagta
     5641 attatattta ataaataaga gctaatttaa aaaaagatat acagttaa
//
LOCUS       DACXMZ010000226         5660 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.227, whole genome shotgun sequence.
ACCESSION   DACXMZ010000226 DACXMZ010000000
VERSION     DACXMZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.227"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            265..1536
                     /gene="serS"
                     /locus_tag="JD016_16630"
     CDS             265..1536
                     /gene="serS"
                     /locus_tag="JD016_16630"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAU5001245.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            1860..3365
                     /locus_tag="JD016_16635"
     CDS             1860..3365
                     /locus_tag="JD016_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAU5001246.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            3479..4177
                     /locus_tag="JD016_16640"
     CDS             3479..4177
                     /locus_tag="JD016_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAU5001247.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            4480..5550
                     /locus_tag="JD016_16645"
     CDS             4480..5550
                     /locus_tag="JD016_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001248.1"
                     /translation="MKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGLIGALIS
                     MVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILIVMISGA
                     GSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAVGLISLF
                     KHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIESKKDAF
                     WGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSVALILGI
                     YTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPYTGYMGL
                     LILVCMVYKAITCRKKSTQSQE"
BASE COUNT         2292 a          559 c          942 g         1867 t
ORIGIN      
        1 gaaaaagtca aaatagataa ttgattagca aaatagatat aattataatg aagttaaatc
       61 tagcaatatt ctcctaaaat taaatttcat ttatttaatt aaatataatt tgacaatggg
      121 caaaaaagtg agtaaaatat aaataaaata aaaataacag gttcggataa agaaaaaggt
      181 ttcttaacct caagcatgaa gtatattatc taagttattt gagattaaga gataaatcca
      241 atcttaggta ctgaggagga aaaaatgctt gatataaaaa gaataagaga aaatttagat
      301 tgtataaaaa aagcaatgga aagaaggggc gaaaaagact ttaaccttga tgaagtagtt
      361 aagttagatg atgaaagaag aaagattctt caagaagttg aagttatgaa aaatgaatta
      421 aatacagcat ctaaaaatat accaaatctt ataaaagaag gtaaagatgt agaaaatgaa
      481 aagattaaat taaaagagtt atctgataaa attaaggtaa tagaccaaaa tcttaaagaa
      541 gtagaagata agatggaata cctattaatg agaataccaa atgtaccaca tccagaagta
      601 ccacaaggtg aaactgatga agataatgtt gaagtaagaa cttggggaaa gactactacc
      661 tttgattttg aatctaaggc tcattgggaa attggaacag aattaggtat acttgatttt
      721 gaaacagcta gtaaaataac tggttcaaga tttactcttt acaaaggatt aggtgctaga
      781 ttagagagag cattattaaa tttttactta gacacgaata caaaagtaaa tggatataca
      841 gaagttattc caccatttat ggcaaataga aatagttttt taggtacagg acagttacct
      901 aagtttgaag aagatatgtt taaaatagaa ggtatggact attttatgat tccaacatca
      961 gaagttcctt taactaatat acatgctaat gaaatactta aatttgaaca attaccgata
     1021 aattatacag cttatactcc atgttttagg tcagaagctg gttctgctgg aagagataca
     1081 agaggtttag taagacagca tcagtttaac aaagtagaaa tggttaaaat agttgcacca
     1141 gaagaatctt acaatgagtt agaaaagctt actaacaatg cggaaactat gctacaatta
     1201 ttgaatctgc cttatagagt agtaaagata tgtacaggtg atttaggatt tacagcttct
     1261 tttaaatatg atgtagaagt ttggatgcca agttacaata ggtatgttga gatttcatct
     1321 tgttcaaact gcgaagattt ccaagctaga agagcaggaa taagatttaa aagagataaa
     1381 gattctaaag ctgaatatgt acacacatta aatggctctg ggttagctat tggaagaagt
     1441 gttgcagcta tattagaaaa ttatcaacaa gaagatggtt ctgtggttgt tcctgaagtt
     1501 ttaagaccat atatgggagt ttctgttatt aaataagagt taaattatgt atttttaagc
     1561 aatacaataa tatagaggta ataaaaaata attaatataa aagtgatact tctaatatag
     1621 aagtaataaa aaaaaagtta atataaaagt gatacaataa tatagaagta ataaaaaata
     1681 gttaatgtaa aagtgataca ataataatag aagtaataaa aaaaaataat taatataaaa
     1741 atgtgtacaa actaatgaat attgatatag taagctatca tatgaaagag tatgtacaca
     1801 ttttttttac taatttttca caatatgaac ataaataatt tataggaaag gaatctttta
     1861 tgaaaattca aaatactgag tggggatata ttgaatggaa acatacttat gatgaaaata
     1921 atccaaaaca agctatgaat atatacattg cagtaactat gcctggtaag aaacatttta
     1981 atcatgttca ttatggtcaa gagcagatga tatatatttt agagggagag ggtttgtata
     2041 ttataaatgg agtttggaag ccattttatc aaggtatgat tttttatata gaatctggta
     2101 gcacacatga aactataaat acaggtgata gagaaataaa agaacttatt gtttcaaata
     2161 atgtagatga tgtaggagag agtgaagtta tagatattaa tcctaataat tatttaaaga
     2221 agaccttgat aaattatagt gaaagtacat taaatttgta tgcagcagta gaatcaattc
     2281 gtggtcaatt tatagacccg tttaaaattc cattaattat atatgatgat tcatggaata
     2341 tagttttaaa aaatccttat tttccacttt tttgttttga aaaatgtaat cctatgaaat
     2401 ttcctcaaaa ttgtgattgt atgaatcaaa aaagtagcaa tcaatttgta tgtgaatatg
     2461 ggataactat atataatata ccaatactgt acaaaagcaa tagcataggt gttataagag
     2521 gagggtatgt actattatct gatttgaatt tagatacaga gcataataat ttatatgata
     2581 ttccagaggg agctgctaga agtataaaac gcctcttgaa acaaatatct aaaaatataa
     2641 taaatttttg ctcttttaat gatataagaa aggatttaca agaaaaagag aagactatag
     2701 caagaacata tcattatgga gagcaacttg aaatgaattt aaaagtagct caagatatgg
     2761 ttactaattt aagaataaat catcactttt tatttaatac attaaattca atggcaagca
     2821 tagcactaga tgatggtagt tatgatttgt atagtgcaat aatagattta tcaagaatgt
     2881 ttagatatac aatgaggtct gatttaagat ttgtagaatt agaatctgaa atattataca
     2941 ttaaaaatta tttgaattta cagaaattac gttatggaga tgcacttaaa gtaaaatatc
     3001 ttattccaga gaagttatat aacctttctg tgccttttaa ctttattcaa cctattgtag
     3061 agaatgcatt tacacatgga tttagagata ttgacacaga aaagcgaatt gaaataattg
     3121 caagattaga ttctcagtat gctattatag agattcataa taatggaact atcttagatg
     3181 gaaataatat agacaaaata aaggcaggta ttcgtagtaa taatggtcat ggtttgtctt
     3241 taatatatac aaaattcaca tctgcttatg gaaataattt tgatatggat ataaagtcga
     3301 gtgataatga aggaacttac ataatgatta aaattcctat tgaaaattat aaggaatatg
     3361 tatagaactt gtgaaaaatt aataagtttt ataaatgtta agaaatatat tagatgtttt
     3421 tatgttgttt tatatatctt acttttttat attattaagc taaaagggag gtattctcat
     3481 gagaagagca ctaataatag atgatgaaga agctgttcta aaaataataa cacattttat
     3541 agaaatgaaa gatatgccaa taaaaattgt tggtaaagca acatctggtg atgaagcagt
     3601 agataaaatc attggtttag agccagacat agtttttata gatattcaaa tgccaatata
     3661 caacgggtta gaagtaatag aaaaaacatc tcatttatct aaaaaaataa actttgtagt
     3721 aataactgca tttaactatt ttgaatatgc tcaaaaagct ttgagattaa atgtaaaaga
     3781 tatactcctt aaaccattgg atatgaaaga atttactaag tctgtagaaa aaattattgg
     3841 atatcaatat accaataatg atttattaaa tgagatactg gaatatataa atctaaacta
     3901 tagcaatgac ttaaagttaa atgattgtgc tagattattt ttaaccaatc catctaatat
     3961 atctaggatt tttaaaagta atttaaatac aacttttata tcatatctaa atcatataag
     4021 aatagaaaaa tctattgaat tacttgaaaa tactacaaaa agtataaatg agattgcaga
     4081 aatagttgga tataatagcc taaataactt ttataaaaac tttaagatag aaaaaggaat
     4141 gactccaaaa gtttataaac tgaacactaa aaattgaaat tgataaataa aaagtcaata
     4201 tagataattg tttgagtgtg tctgaataag tatactttaa ttatgataaa aattaaagcg
     4261 ctttaaacat atttaataat attaaacctt ataaaagctg tacctattta tataaaaaaa
     4321 tatatataaa taaaaagaaa aagatataca aaataaaaaa agtgaattaa atactgtttc
     4381 aaaaagacag aattttcatt taattcactg agagtttata tttcttaaag gaaggtaact
     4441 ttataatgga ggtaatttaa tatgtcagac aaagtgaata tgaaaaatat tatctgtatt
     4501 gcaggcgctt atctagcaac agcaattggt tctgggtttg ctactggtca agaaattcta
     4561 caattttttg cctcacaagg gtatatgggt ttaattggag cattaatatc aatggtattg
     4621 ttttcatttc ttggtgctga agttataact agaggtagag aattaaaact taaagaacca
     4681 acaagaattt acacttttta ttgtgggaag attttaggaa aattttacga atggtttagt
     4741 ccaatatttt tatttgggat tcttatagta atgatttcag gtgcaggctc aactttaact
     4801 gaatactatg gtataaatcc ttacttagga agagtattga tggctgttgc tactttaatt
     4861 acagtgtcta tgggtttaga aaaattagta agcatacttg gacgtattgg accagtgata
     4921 gcagtattta caattgctgt tggattaata agcttgttta agcattttga cggtttatct
     4981 atggttggag aagctatgaa aaatattgaa gttacaaaag catcagaaag tccaatcgtt
     5041 tctggtgtaa tatataatac attaaatatt attgtaatga ttgttttttt aacaggaatg
     5101 ggagcttcta ttgaaagtaa aaaggatgct ttttggggag gtttattagg tggaatagtg
     5161 tttatgactg ctggtatagt tatgtatttg gctatgattt cagatatagg aaatttgtat
     5221 atgaaagaaa taccatcact ttatttagca gataatatat ctccgattgt aggtgtatgt
     5281 ttttcagttg cattaatatt aggtatctat acaacagctg ttccactttt atggtctgta
     5341 accaatagaa ttgttgaaga tgaacatcct aaatttaaat tggtaacaac agtaatagct
     5401 attttggctt gtataggagg ttttttaccg tttggtaaat tagtaaacat tttatatcca
     5461 tatacaggtt atatggggct tttaatttta gtctgtatgg tgtataaagc aattacatgt
     5521 agaaaaaaat caacacaaag tcaggaatag agccacttat taatagtttt tatttaaaaa
     5581 aattttttat ataatccgaa tttaaaaagt caaaatagtt aaataaattg caaaacatga
     5641 taatatattt aattaaaata
//
LOCUS       DACXMZ010000227         5653 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.228, whole genome shotgun sequence.
ACCESSION   DACXMZ010000227 DACXMZ010000000
VERSION     DACXMZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.228"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            9..860
                     /locus_tag="JD016_16650"
     CDS             9..860
                     /locus_tag="JD016_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAU5001249.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(993..2606)
                     /gene="ggt"
                     /locus_tag="JD016_16655"
     CDS             complement(993..2606)
                     /gene="ggt"
                     /locus_tag="JD016_16655"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAU5001250.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLGLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2831..4768)
                     /locus_tag="JD016_16660"
     CDS             complement(2831..4768)
                     /locus_tag="JD016_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5001251.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEIEKINLHIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4755..5522)
                     /locus_tag="JD016_16665"
     CDS             complement(4755..5522)
                     /locus_tag="JD016_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001252.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
BASE COUNT         1901 a          912 c          631 g         2209 t
ORIGIN      
        1 tacattcttt gaaaaacatt atttcagcaa aagaacttat atcaaaacta gaaaaaaatg
       61 ataatctagt tgtaatagat tgtagatttg atcttatcaa tagaacttat ggtattgatt
      121 cttataaaaa aggacacatc aaaggcagtt ttatcttaga tatagacaag gatttatcat
      181 ctcctacaaa agaacatggt ggtaaaaatc ctttacaaga ccctttaata ctaaaagaaa
      241 aattagaaaa aatgggtgtt gataatgaca ctacgattgt aacttatgat gatggagatt
      301 taaatggagc ttgcagatta tttttccaat taaaacattt ggggcttaaa aatgtctatg
      361 tactggatgg gggaataaca tctttcgtaa aggaaggtgg agaattggaa gagaaagtga
      421 atataccaag ttgtacaggt aaggaaatta gaccaaatat aaacaatgat ttagttgttc
      481 caatggaata tgttaagtct aaattataca aaaaagatac tgtaataata gattgccgtg
      541 ctaatgagag ataccaaggt ttagttgaac ctgcttactc aaaagctggt catataccaa
      601 gtgcaaaaaa ttatttttgt aaggatttaa taaaaagtga ctttgaaaat ggttcactta
      661 aagatataga atttttaaaa aaattcttta aagatttaaa taattatgat gaagtaattt
      721 tatcttgtgg ttcaggaata tctgcttgtg ttaacagtct tgctcttaga gaactggata
      781 tacctcacaa gatttatatt ggtagcttta gtgactggat tagttatgat gacaatgaaa
      841 tcaaaactgg tcaagaataa ggctcataat ctatataaaa tcagcatatg atagcttaaa
      901 ttttgtaaag gcgtcagtca ttgtagactt tcgcctttac ttattatttc atatactctt
      961 taaatttatt atatatattt tagttgataa tattaataaa ctgcaatatg accatcacat
     1021 cttgactccg tcccacaaat ataactttcc tgttctgttt taaatattat ttgacctcgt
     1081 cccatgtcga cagtatcatg aattactttt atttcatgac ccttttcaat taatgaatct
     1141 attatatgct ttggcatctc tggttcaact tctatttcct ttccttttat ccactgccat
     1201 cttggagcat ctagcgcctc ttgtggattt aatccaaaat caatcatatt tgttaacact
     1261 tgaatatgtc cttgtggttg cataaaagct cccattacac caaatggtcc tattgcttta
     1321 tcttctttac ctaaaaatcc aggtattata gtatgataag gttttttaaa tggttttact
     1381 acattgtggt gtttagggtc taaattaaaa ttatttcctc tactatgtaa agcaatccca
     1441 gttcttggta tcacaattcc tgaaccaaaa tttatgtaat tgctctgtat atatgaaacc
     1501 atatttccat ccttatctgc agtacaaagg tatactgtac caccacaata tggctctcct
     1561 gcagttggat acaaagcctc tttattgtca atcaataaac ttcttttttt ggcatactct
     1621 tggcttagta attcctgaat ctttaccttc atatgctcta tatcagttac atatgtttta
     1681 gaatctgcaa aagctaattt taaactctct ataattttat gtatgtcttc tacattttct
     1741 atatttcctt ctaattccag ttcttttaat atgttaagtg ccattaaaac tgttattcca
     1801 tgaccatttg gtgggatttc aaatatttta tatcccttat attctgtact tataggttct
     1861 acccatgttg gataaaaaca ctctaagtca ctctttctta tagccccatt aaatttttta
     1921 gaatattcat ctattttatc agcaagtctt cctctataaa agctttcagc ctgagtattt
     1981 gctatctctt ctaatgtact tgcctgttct tcacatataa atatatctcc agcctctgga
     2041 gctttaccat ctttagaaaa tgtatcaaac catggtttga actcttctcc aataagttct
     2101 ttttcataga gttcatatga ttttttccat accttagcta cattaggaga tactacgtat
     2161 ccctctcttg catagtttat cgctggagat aagcattcca ttaagccaag tttaccatat
     2221 tttttattta attctgacca agctgctggt attccaggta cagttactgc tccaaaccca
     2281 tattttggca tttctttcat attattataa ttttttaatt ccatattttc tggagcaaag
     2341 ccactagaat ttaaaccata caatcgtttt tcttcctcta tccaaacaag ggcatatgca
     2401 tctcctccaa tcccattgct tgtaggttct actactgcta atgtggcggc tgttgcaact
     2461 gctgcatcaa cagcatttcc accctttttt agtatttcaa gtccagcttg agatgctaat
     2521 ggtgttgatg ttgcaactgc tccattttta gcatacacga catttcttat tgatgagtat
     2581 ttatgttggt aggcattaaa cttcataaat ttcagctcct cgtcattttt tatttgatat
     2641 tctgcttaga tttattatat aacatcaagt aatgtaactg atacaaaatg atataaaata
     2701 tagacttttt cctaacttta attttatctt taaataaata tcttaaactc aattctagta
     2761 ttttctatct gcacttaaat aagttatttt attcaaaaca tatatctaat tcttttgact
     2821 taaaaataat ttattctttt atcatgttta gagcatattt taaagttaaa aaataatata
     2881 taaaatatcc tataaaataa atgactagag ttatgataat tggtacaagt aatgaccttg
     2941 gtataaactt ttgtgctata gacatagcaa ataaattatg aactgtacct acaactaatg
     3001 gcattaaaaa tataactttt aactgttttg atattatttt ctcaatatct ttattatttg
     3061 ctccaagttt tattaacata atatatcttt ccttatcatc atatatattt gataattgtt
     3121 taaataagat tatactacct gtacataaaa gaaatactac agacaaaaat atgcctataa
     3181 acagcattag accacttaat tttatcaagt tattataact ttcaatactt gatgtaaaat
     3241 taaaaggata agtaaatttc atattttcac ttactatatt ttttaatttt aaagtcaaat
     3301 ccagttcttt tttttcattt tttatatcaa ttaatcttat taatctagaa tttccatgta
     3361 attttaactg attaaatacg tcatctttta ctacaattaa atcaaacgta gcttgtgaat
     3421 ttatttctgg ctccaagatg cttttttgta ctttaaacag atttatatca tactcatttg
     3481 tatctttaat atgcaagttt attttttcaa tctcaaagtc tttaaaaaat atttttttgt
     3541 atgaatcaga aacatagtac acagattcct ttgaagttaa ttcttcataa ttgttattct
     3601 gatattgtct taatttttta aaatcacttt ctctaataat atctacagac gttctatctt
     3661 ctatataact atttccattt ttataataca tttcaatatt tatatctttg tctataagtt
     3721 ctattgtttt atcaaagatt atcttattat cttttttata tttatataat aatttgtcaa
     3781 tttcgttatt tacataagaa tttcctaaat ttatggtata actgtattta taattttcac
     3841 ttatgttcct atctatatcg taataaagtg atatggtaaa ccctagcata gttacacttg
     3901 tagctattaa tattgctatt acagccaata cactactatt gctctttaat ttaaatccaa
     3961 gttcagaaaa tgcaattaaa ttctgtccct tataataaaa actttttctc ttcttaacag
     4021 catcaataat catatacatc gttgatgaaa aaaataaaaa agttccaggt attattgtaa
     4081 ctaatataaa aactgaaagc ataatatttt cagcaataaa ataactaatt gacattaaat
     4141 aagaaatagc tattaataaa acaccaagta cacattttaa ctttataaat tttttaaact
     4201 tatacttttc tggatattta ttaaataact ctataattct cttatttctg ataactatag
     4261 tatttcttat tccaataact ataaatatta ttaaaaatac cgctagtgtc tgaactaagg
     4321 cctttatact taagttcata tttactacta tcatttcatt cattaatttt acaagcatca
     4381 tagtcataag ctttgaaaat aacaatccta aaaatatacc tattaatatt gcaactatac
     4441 ccatcaaaaa agtttcatat aaaaatattt ttcctatctg atttttcctc attccaagta
     4501 aactataagt tcctacttct cgcctacgtc tattgaaaaa aaatgagttt gaataatata
     4561 taaataaaaa tacaaatacc actattacaa ttgaacttgt attaatactc atcctagttt
     4621 tctctccaag tgaactcaac gcttcattat actctataga tttaaaacta aaaaatacaa
     4681 atacactaaa tacaattgaa ataaagtaca ttaaataatt aaaaaaacta ttctttacat
     4741 tttgcattgc aattctaaat aaagtcattt ttattaactc ctccaattaa ggaaactgta
     4801 tttactattt tattatagaa ctctctgtta ctttctccct ttacaatttc tgtgaatatt
     4861 attccatcct ttataaagag tattctattg caaaaactcg cacttatagc atcatgagtt
     4921 accatcaata ttgttttatt gctgtctttg tttaatttta atagacaatt caacaaatcc
     4981 ctagcagatt tagaatctaa tgctcctgta ggctcatctg caagtactaa tgatggtttt
     5041 gttgctatag ctctacatac agctcctctt tgcttttgtc caccagaaac ttcgccaggg
     5101 tatttatcta atatagattc tatatttaat tcttttgaaa tctcttttat tctattttct
     5161 attactgata ctctttcatt ttttaaagaa agaggcaaca ttatattttc ttttattgta
     5221 agtgtatcca aaagattaga atcttgaaat acaaagccta gattatcacg tctaaaatct
     5281 gccaattcat tttgcttcat tttaatagta ttctttccat ttatataaat atctcctgat
     5341 gaaggtaaat ctattgtaga aataatattt aaaagtgttg atttaccagc accggaagac
     5401 cccataatac caacaaattc tcctttataa accttgaaag ttattcctct tagagcttca
     5461 tgttttgaac cttttcttcc atactccttt tttatatttt caacagataa tatttcttcc
     5521 atagttaata atcacctttc atcacaatta gttatttatc aattctcaca aatatattat
     5581 atatattaaa atggctttta aacatttaat tatattaatt taaggttaca ttttcgtaag
     5641 tattaattat att
//
LOCUS       DACXMZ010000228         5619 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.229, whole genome shotgun sequence.
ACCESSION   DACXMZ010000228 DACXMZ010000000
VERSION     DACXMZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.229"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1501
                     /gene="polA"
                     /locus_tag="JD016_16670"
     CDS             <1..1501
                     /gene="polA"
                     /locus_tag="JD016_16670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAU5001253.1"
                     /translation="KPYNINLENMYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVK
                     SKEELLGKGVKSKNYEDLEFEDLAEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVE
                     MPLVEVLGHMEYEGIKVDKSILDELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGV
                     ILFEKLELPIIKKTKTGYSTNADVLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNI
                     INPESNRIHSSFNQTITTTGRISSTEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDAD
                     YSQVELRVLAHMSQDENMIEAFKHHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFG
                     IIYGKSDFGLADDLNIPVPKAKEYIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNR
                     RRYIPEIKSSNFMERNRGKRFAMNAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQ
                     VHDELIVEAIDDEIDIVKKIVKEEMENAVNMDVHLDVDLNVGSSWYETK"
     gene            1510..2112
                     /locus_tag="JD016_16675"
     CDS             1510..2112
                     /locus_tag="JD016_16675"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAU5001254.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            2105..2659
                     /locus_tag="JD016_16680"
     CDS             2105..2659
                     /locus_tag="JD016_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAU5001255.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            2659..4374
                     /locus_tag="JD016_16685"
     CDS             2659..4374
                     /locus_tag="JD016_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAU5001256.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            4447..4659
                     /locus_tag="JD016_16690"
     CDS             4447..4659
                     /locus_tag="JD016_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAU5001257.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            4860..5459
                     /locus_tag="JD016_16695"
     CDS             4860..5459
                     /locus_tag="JD016_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001258.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
BASE COUNT         2408 a          536 c         1010 g         1665 t
ORIGIN      
        1 aaaaccatat aatataaact tagaaaatat gtattttgat ataacaatag ctgagtactt
       61 aatagattca acttcatcta cctcatatga ttgtagtgca atagctatga agtatttatc
      121 aaaaaaggtt aaatcaaaag aagaattatt aggaaagggt gttaaatcta aaaactatga
      181 ggatttagaa tttgaggatt tagcagagta tatgggtcaa ataatatgca cagttaaaga
      241 aacagttcct atgatggaaa gaagtctaat ggatatgagt atggatggtc ttttttatca
      301 tgtagagatg cctttagtag aagtcttagg tcatatggag tatgaaggta taaaagtaga
      361 caaaagtata ttggatgaat tgagtgttga atttaaagaa ataatagcta cattagaaaa
      421 ggaaatttat gaattatcag gagagccatt caatataaat tcaccaaaac aattgggtgt
      481 tatattattt gaaaaattag agcttccaat aattaaaaaa actaaaactg gatattcaac
      541 taatgcagat gttttagaaa aattaagaga taagcatcca ataatagata agataactga
      601 gtatagacaa atagtaaagt taaattctac ttacgttgaa ggacttttaa atataataaa
      661 tccagaaagt aatagaatac attcatcatt taatcaaact ataacgacta caggaagaat
      721 ttcatctaca gaaccaaatc ttcaaaatat acctataaaa atggaaatgg gtagaaaaat
      781 aagaaaagtg tttattgcag atgataactg taagttggtt gatgccgatt actcacaagt
      841 agagctaaga gttcttgcac atatgagcca ggatgaaaat atgatagaag catttaaaca
      901 tcatgaagat attcatacca aaacagcttc acaagttttt aatgttccta tggaagatgt
      961 tactagtgaa ttgagaggtg cagctaaagc tgtaaacttt ggtattatat atggtaaaag
     1021 tgactttggt ctagctgatg atttaaatat acctgttcca aaggcgaaag aatatatcga
     1081 aagttatttt gctaaatacc ataaaataaa agagtttatg gacacaacag tagaaaaggc
     1141 tagtgaggat ggatatgctg taacaatttt aaacagaaga agatatatac cagaaataaa
     1201 gtcaagcaat tttatggaaa gaaatagagg aaagagattt gcaatgaatg caccaataca
     1261 aggaagtgca gcagatataa taaaaattgc tatgataaat gttcataaaa aattggaaga
     1321 gaacaatttg aaatcaaaac ttatactaca agttcacgac gagttgatag ttgaagcaat
     1381 tgatgatgaa atagatatag ttaagaaaat agtaaaagaa gaaatggaaa atgctgtaaa
     1441 tatggatgtt cacttagatg tagatttaaa tgtaggtagt tcttggtatg agacaaagta
     1501 ggtgatttta tgttgatatt aggacttaca ggaggaatag gctgtggaaa aagcagccta
     1561 tcaaatatat ttagaaatct taacattcct atagtggatg ctgatattat atccaggaag
     1621 atatttgagg ataagttgtt attggaaaaa gtatttgtcc attttgggca aagtataaaa
     1681 aatgatgatg gaactctaaa tagaaaagct cttgggaaaa tagtttttag tgatgaagaa
     1741 aaacttaagg aattaaataa cttgactcat ccacgaataa gagaaaaaat aataagtgaa
     1801 atagaaaaat tgagaaaaaa aggcgaaaat atagtagtgt tagatgcggc aatattggta
     1861 gaaagtggtt ttttagatat ggttgacaag ctattagtag ttacttgtaa acaagaagta
     1921 caaattagta gaatacaaaa gagagataat tgcagtgagc aggaagcact tagtaggata
     1981 aattcacaga tgagtcagga agaaaaatcc aaatatggtg attatataat agataattcg
     2041 ggaacaataa ctgaattaga aagtaaagca cataaattta ttgagtacat gaaggagaat
     2101 tggcgtgaat agtaagaaag tattgattct atctattttt ataatcttat ttggggcact
     2161 attaatggaa agcaaagtaa tacataaatt tttatatcct aaaaaatatt cagagtatgt
     2221 agaaaagtat tcgaaagaat ttaatttaga tgaaaatata gtttacagtg ttattaaagc
     2281 cgaaagtaag tttaatagtt ctgctgtttc aaaaaaggaa gcaaaaggat taatgcaaat
     2341 attagacata actagagatt ggggagcaga ggaactaaat ttaaaaaatg tggatatttt
     2401 cgacccagag actaatataa gacttggctg ttggtattta agtaagttat acaaagaatt
     2461 tggtaaatta gatttagtga tagctgcata taatggtggt tcaggtaatg tgaaaaaatg
     2521 gttagaaaat aatgaatata gtaaagatgg cgaaaatcta catgatatac cttttaagca
     2581 aacttcaaaa tatgtagaaa aagtaaaaaa taattacgaa cattataata agatatatgg
     2641 caagaaagga aaaaactaat gaagagaata aaagtcttaa cagtcgtttt agctataact
     2701 cttatggtag ctggttgtag taatactaaa acaaaacaaa gtagtagtga ttcaagttta
     2761 caaagtcaaa gttctataga tgctaagtat ataaacttaa caatggtaac tccaaaaact
     2821 ataaatccta taactaatac tgataagtct gttggttata taatgaattt agtttatgat
     2881 agtttattta ctatagatga gaactacaat gtaattcctc aattagttaa ggagtataat
     2941 atagctcagg atggaatgag tatagatata aagctaaaag atgcaaagtg gcatgatgga
     3001 aaaaatgtta cttcaaatga tgtaaactat actattggtt taattcaaaa aagtgcagat
     3061 agtccatata atgaatttac taaaaatata gcttctgtga atattaaatc agataaagat
     3121 tttacaataa aatttaaagc tagatatgct ttttcaattg atagtttaat ttttccaatt
     3181 gtatctcaaa atcatttaga ttcaaaagat gtaaatgaca agaataataa tatgatagga
     3241 aatgggaaat ataaaataga atcatataca gaaagagaag gaatggtttt atctgtaaac
     3301 aaagattact atgaagaagt tccaaaaacc atgaagaaca taaaagtagg tatggtgcct
     3361 aatgaagatg caaggacatc tatggttatg gctcttgata gtgatataac caatgttaca
     3421 ttaaatgatt taagtaagtt tcaagaaaaa gagtttaata taacaaaata tcaaggaaga
     3481 gattatgagt gtgtattatt taattataat aatccttttt tcaaagatgt taattttaga
     3541 aaagcaattg cacactctat agataaagat agaattatta gtgaaggtta tatggatgat
     3601 gctactccag ttaattttcc acttaattca aaatcaaaat actataatag tgaaatgaag
     3661 gatttagagt ttaataaaga taaggcgata gagtgtcttg ctaaggtaga atatgcaaat
     3721 gtaaactcag taaatcaaaa tgacaataaa gtaaaaaatc agaaaaaatc agctaaaaag
     3781 aactcaaaga agcttacgcc agaagaagaa gcaaaagcta aaaaagcaga agaagataga
     3841 atcaaaaaag aagctgaaga gaaaaaaaat agagaaaaag aggaagtaaa gaaaagtctt
     3901 tctgaaatga acttaaagat aatagtaaac agagataata gtgagagaat aaaaactgca
     3961 aacataataa atgaaaactt aaaagctata ggaataaata gtacagtaaa tcagctgtca
     4021 gataaggata tggaaaatgc tcttaattct aaaaattatg atttagcatt agttggatgg
     4081 aaactatcaa ttataccaga tgcaagtagt attatagcga gtagtggata tactgatgat
     4141 aagttaaatg gatatatgtc tgcacttaca agttctacgt ctgaaattga aacaaaaaaa
     4201 gcatacaaag atgtacagac atatataaaa gataatgcaa catttataag tttagctgtg
     4261 agaaacaact atattgtaag taatagtaga ttaaaaggta aaattactcc aaatgatttt
     4321 gatgtatatg agggaatatc taatttagat ataaaatcga atgaatcaaa ttaaatgatt
     4381 aatttctatt taaaaagtat aaactatatg atagaatacc cttatagggt ataaggaggt
     4441 aattaaatga agaagaaact attagtagaa ggtatgagtt gtggtcattg tgtaaaccat
     4501 cttaaaactg cattaacaga agatatagat ggtattgaag ttttagatgt agatttagaa
     4561 aataagtgtg catctgtaga tatgaaagat gatgtatcta ttgaaaaatt aaaagaagtg
     4621 atagcagaat taggatttga actaaaggga attgaataga aattgataaa ctaaaaaaag
     4681 acctctattg ggggagtaat agaggtctaa aaatggggga gattgagtat gtttgatttt
     4741 acattactat tatctcccct tttaaattaa tatatacata aaaagtaaaa aaattttgta
     4801 atgactcaca tttcttgagc tttttgaata tactataaat agctaaggga gggagtatca
     4861 tggaatctag gggatataaa gaggatttaa aaaaaatgat acttgatatg gcccacaaag
     4921 aacttgagag ttatgtatca caaaaaggag caaaaaaata ctttgaaggt aacttggata
     4981 agattattaa taataatata aaaaaattca atcaaagtat tggtcttaat agagtatatt
     5041 taaaaatgtt aaaaaagtgt gcatatcaag aaagtgtaac ttcaatatca aggactataa
     5101 agaaagaagc attatttaaa tcaaaaaaag agctatttgc tttttcagag tacttaaaac
     5161 ttgatattaa caagcgactt tcatataatc aaattttaag aaaaatatca agatatatat
     5221 actacaatag aagttcatat gctcaaaaat acgttgtttt caaaagagga gatgaagaat
     5281 atcttttaga accagagaaa ataaaagatg aattaatatc aagctatagg tcaaagacta
     5341 gagaagatat gaggtcaata gcaaaacttt tggatataga agtagaagat gattataatg
     5401 ctgaagatat tagaaaaaaa gtaataaatt atattatcaa agaaaaactt aaaagttaga
     5461 gttttaaaga ataagttctg ccaatttgca tgactaagta taacttgggt ataaattttt
     5521 tatgcatttg aaagagctta ttcttttttt gacatattct tttaaataat ttaccatgat
     5581 ttggtaattg gtcatcatca ttcaaatttt taacttaat
//
LOCUS       DACXMZ010000229         5590 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.230, whole genome shotgun sequence.
ACCESSION   DACXMZ010000229 DACXMZ010000000
VERSION     DACXMZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.230"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            18..416
                     /locus_tag="JD016_16700"
     CDS             18..416
                     /locus_tag="JD016_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001259.1"
                     /translation="MKKLDAESLEKMAKQKNIDKDKIEKMADSYKGKSESELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDTIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            complement(539..1555)
                     /locus_tag="JD016_16705"
     CDS             complement(539..1555)
                     /locus_tag="JD016_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAU5001260.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(1808..2008)
                     /locus_tag="JD016_16710"
     CDS             complement(1808..2008)
                     /locus_tag="JD016_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001261.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(2042..2221)
                     /locus_tag="JD016_16715"
     CDS             complement(2042..2221)
                     /locus_tag="JD016_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001262.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(2403..2609)
                     /locus_tag="JD016_16720"
     CDS             complement(2403..2609)
                     /locus_tag="JD016_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001263.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(2657..3082)
                     /locus_tag="JD016_16725"
     CDS             complement(2657..3082)
                     /locus_tag="JD016_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001264.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            3254..3481
                     /gene="acpP"
                     /locus_tag="JD016_16730"
     CDS             3254..3481
                     /gene="acpP"
                     /locus_tag="JD016_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAU5001265.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(3625..4638)
                     /locus_tag="JD016_16735"
     CDS             complement(3625..4638)
                     /locus_tag="JD016_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAU5001266.1"
                     /translation="MIYMIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKR
                     IAKDEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESC
                     DLAICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGA
                     LISLSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWIT
                     NALSNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKNDELKDSFKEHMEEKGLDES
                     FSIDKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNITFY
                     EEK"
     gene            complement(4643..5545)
                     /locus_tag="JD016_16740"
     CDS             complement(4643..5545)
                     /locus_tag="JD016_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAU5001267.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIYEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
BASE COUNT         1849 a          973 c          579 g         2189 t
ORIGIN      
        1 tagaaagggg tttttttatg aaaaaattag atgcagagtc attagaaaaa atggctaaac
       61 aaaaaaatat tgacaaagat aaaatagaaa agatggctga tagttacaaa ggaaaatcag
      121 aaagtgaact tatggaggaa cttataaaaa taggtaaaaa cttagatgga agagatgaag
      181 tagtatcaaa atttaaagcg tttttagatg aaaatcaacg caagaaattg gatactataa
      241 tggaaaaaat ctcagatgca gaaaatcaaa gagatactaa accagctaaa actaagaagg
      301 ctaaacctac taaaggtaag tcaaattcac ctgcaccaaa aaatacacca ggtagtcaac
      361 aaaaaaaatc taaaagttta tttaagaaaa ctaaaaagtc taaccctcat gaatagataa
      421 aaataaacta tttaagcaat tagataatta tttttagtta atctattatt aaatttgata
      481 ttattgacat aaaaaatcag gtagtgccta aggtactacc tgattgattt taatagtttt
      541 atttttttct ttctgtaaca ctttctctat caacaattct ataaggtaat tctattttct
      601 tattttcaat taattcttta tttattaatt ttataaccat tcttattgct actgctccca
      661 tatcgtatag gggttgatat actgtagtaa gtttaggtct atacattcta gctatcttaa
      721 catcattaaa tcctgcaact gatatgtctt ctggaacctt atatccagca tcacatatag
      781 catttatagc acctatggca gcctcatcac ctgttacaaa agccgcatga ggtattattc
      841 catcatctaa tagttctttc atactattgt atccactctc ataatcagtt ccaccatact
      901 taatcaaact cttgtctaat tctatattgt tatttgaaag tgctttctca taaccagcaa
      961 gtctttccat ttctaaaaca gtatcatctt tactagtcat tataaaagct atcttttcat
     1021 gacctttctt tataagatgc tctgtcatat caaaagtagc ttcttcgttg ctagtactta
     1081 ctgtatatat atcataattt ctagcagttt tacttatata tgtcgctggt attccacaat
     1141 tttgtatata gttgatatgt tcttgttcaa ctatccacga aatcataact ataccttcca
     1201 cttgttttgc tctcaataga tttatactct taagttcttg ttccttatca gagtatgtat
     1261 tcgctaaaag aatatcatag tcatacattt tagcaacctc ttctatccca tttaacacct
     1321 cattaacaaa agaatctgaa acttctggaa ctattacccc tattaattga ctcttctttg
     1381 ttactaagct tctagcaagt ggatttggta tatatccagt ctcttttata acctctaaaa
     1441 ctttttgttt gacttcatca gttacaggtt ttgaatcatt tataactcta gatacagtag
     1501 atattgacac tcctgcttgt ttagcaacat cttttatcgt tatattgcct ttcatcttca
     1561 tcctccttat tttactaaat ttatctaaaa ctgaaccaaa tttttgaagg ttcctatttt
     1621 aactacattt atataagttt ttattttacc ataaaagcaa gtacatttaa atacattata
     1681 ttttatatta taccctatat tcaacaaatt taataatatt cattcttaat ttaaatcata
     1741 ttaaaaaata aaataaaaaa atgccggtat aaaataccag cattttttta ttctaatgat
     1801 ttaagtatta gcagccgcaa cctacttcac catcaaagaa tccaccacat gaacaagtta
     1861 ttagcaataa tattaagaaa ggtatccatg acattatgtc aatgtctctc cagcaatctt
     1921 caaaagctaa gaataggaag aatagtacta ttatccacca tcctcctcca ccaaagcatc
     1981 cttcttctcc aaaaaatcta tctaacataa caaagccctc ctttttttaa taactgtgtt
     2041 cttattctaa gcatccacca cagcagcaaa gtattaataa aactattagg aatggtatcc
     2101 atgccattat gtcaaattct gcccagctat ctccaaaagc taagaatagg aagaatagta
     2161 ctattatcca ccatcctcct ccaccaaaac atccttcttc tccaaagaat ctatctaaca
     2221 ttatacgtta cctcctgttg atattttgtt gagcttttcg ctcctaacat atactatgca
     2281 tttggtttcc attttgctaa tgtattttta ctattttttt aaatttttta atttgaacta
     2341 aaaaaggtaa aaccataact tgtataagtt actggtttta cctatttaat aatcaatatt
     2401 ttttagcaac aacaaccatc atcgaagtta aataataaca atactattaa tataacccat
     2461 atccatatat tgtcatcgca taataagtct tctaaaatgt ctgtgcataa tagtaagaat
     2521 actataacta ttattatcaa cattttattc ccaaaaaatc cttcgcatcc tccaaagaaa
     2581 cgttccatat tgtcttctaa ttttgtcata actaatcctc cttgaatcat taagagacta
     2641 agttttgtct ctttaattaa atatatgtag taatagacag ataagttact tctaaaaagt
     2701 ttttattgat tagtttattt atttcttttt caccaagttt attgtatcta tcaacttcat
     2761 ccagataatc ttttttctcc tctgatatag tatagattaa actttctatt gattttattt
     2821 tgtactcctt atatctttct atcaagaagg atgtttttga attttctatt cctttcttta
     2881 catgcattaa ccttgatatg tagtcttctt tttcattaga tacataactc tccttttcag
     2941 ctacatcttc aaggattaaa ttggtctcca ttacataatc ctctatacgt tgcatatcta
     3001 aagttattga attataataa aaatttactg ctataagtaa aaacattcct actgaagtta
     3061 gtaatagtcc tacatatttc atagcatttc ctcctaagtt atatttgtta tcaataaagt
     3121 ttattcatca aataatactt tattactaca tttataaaat tttattattt tttatagatt
     3181 ccatattgaa aaattcttga tattatatta aactaacatt aaaattaatt aaaactgctt
     3241 taggaggctt aaaatgtttg agaaaataaa agaaataata gcagaacaat taggggtaga
     3301 caatttagat gagataacta tggaagcatc tttaatggac gacttagaag ctgattcatt
     3361 agatgctgtt gaaattataa tggcccttga agatgaattt ggaatagaaa taccagacga
     3421 ggaagctgag aactttaaat gtattggtga tatatgtaaa tatatcgaag aaaacaaata
     3481 gtatcgtttt gtctacaaat ataaatagtc tgtgtaaact tctacataat aaattatatt
     3541 agagttcgca cagactagat tacatttatt tacaatatat aaaatacacg ctcttatatt
     3601 taatttatat actaagtata taaattactt ttcttcataa aacgtaatat tctttattat
     3661 tatatctata gttccatctt gattttgttt tacagtatac ttcattgggt cttcaaaatc
     3721 agttagattc ccttttatgt caaaaccatt atccgttttt atacttcttt ttttaagctt
     3781 tttctcaacc cattttttat ctatactaaa actttcatca aggccttttt cttccatatg
     3841 ctctttaaag ctatccttta actcgtcatt tttaattgaa ttatcaacaa agtcatttat
     3901 atcaacttca tgtttttcct tcagtgtata atttaatata cttcttacat cttcagcttg
     3961 ctttatatca ttactaagag cattggttat ccaattttca gctgtatttt taaatacctt
     4021 ggtcttgtac ttatcatcat ctattttttt agcatttaaa aattctgtta caaacttaga
     4081 ttctgaccct tctttttcag catccttatc taaaagcctt agatgaaact catcatttat
     4141 accacttaga ctaatcaaag ctccttgctt ttgtcttaaa gtctctggga taccaatttc
     4201 atttgaaacc atttgaatat taaatttatc atccacaaat tcaattgaat gagtgtacaa
     4261 ctttttataa tctaatttca gtattgctac actcttttca tctttaatag tgtataagca
     4321 aattgccaaa tcacaagatt ctagtgcagc attaattttc ataacatcaa acaagtaaga
     4381 tgcaatttct ttagaatttt tcaaaaaagt actttcatca tatattattt gttcacaaca
     4441 gtttttaatt aaattgtcat tataatcttt aaaaacacct tttcttaaat cctcatcttt
     4501 tgcaattctt tttatagctt tctggaaaaa accatctact tcttgattaa ctttgccttc
     4561 aaagtcattt aatattggaa catcactatt tttgtccaaa acatgtatta taaatttatg
     4621 tattatcata tatatcaccc cattatctta atttcgatat taactctttc atatcctctg
     4681 gtatatctgc tttaaatttc aatttttctt ttgttcttgg ttgttcaaac tctaacttat
     4741 atgcatgaag tgcttgccta tttattagct cttcatctac ataaccatac aactcatcac
     4801 ctattattcc atgacctata tgagccatat gtactcttat ttgatgtgtt ctaccagttt
     4861 ctaagcttac ttctaataca gttgcatttt ctagtctttc tattacttta tagtgagtaa
     4921 cagatgactg tcctctttca tctattatcc tctttattga atcttctgta ggtctataaa
     4981 ttggctcatc tatagttccc tcattttctt ttataattcc atcaactact gttatatact
     5041 ttttttcaac tttattttca ctcattgctg tagacaaagt atgatgtgca taagcatttt
     5101 ttgctactat aactaaccct gaagtattca tatctaacct atttacaaat cttattttta
     5161 cgttttcttt ttgattatct atatagtaac taataccatt tgctattgtt ttatcatagt
     5221 ggctttttgt tgggtgaact accataaacg gaggtttatt caccataatt atatcaaaat
     5281 catcataaat tatctgtaaa tttaaatctt gaggttcaaa atttgctttt tcctcgtata
     5341 tttttacttc tattaaatca ccactataca cctttaaact cggtttttta tatactccat
     5401 ttactaaaac actcttttcc ctcttcattt ttgataatga cctaactgag aaatttagct
     5461 tgtccaataa aacttctttt aaagtcattt cttcttcatt tgtatacgat ataagattat
     5521 atctttgatt ctctttttta aacaaacttt tacctacttt cttttatatc ttcctacttt
     5581 tttatatctt
//
LOCUS       DACXMZ010000230         5583 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.231, whole genome shotgun sequence.
ACCESSION   DACXMZ010000230 DACXMZ010000000
VERSION     DACXMZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.231"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            55..1236
                     /locus_tag="JD016_16745"
     CDS             55..1236
                     /locus_tag="JD016_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAU5001268.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1378..2295)
                     /locus_tag="JD016_16750"
     CDS             complement(1378..2295)
                     /locus_tag="JD016_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001269.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2548..3342
                     /locus_tag="JD016_16755"
     CDS             2548..3342
                     /locus_tag="JD016_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001270.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3343..5403
                     /locus_tag="JD016_16760"
     CDS             3343..5403
                     /locus_tag="JD016_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAU5001271.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2136 a          631 c          916 g         1900 t
ORIGIN      
        1 attagaaata ataaataaat aatacattgt acttaaaagg gggagaaatt taagatgaac
       61 aaaaaatata ttaaagcagg gaatttaatt gatggaaccg gaaatagtat tatgcaaaat
      121 aagggtataa ttattgatgg agatactata gttgaaattg aagatataag agatgattta
      181 aatggatatg atgtgtacga ttacttagat aaaactgtta tgccaggcat tatgaattgt
      241 catgttcact tgacaatgga gccagttgga aatccaaaga tatattatga taatgtatca
      301 gatgtagagc ttgtagtaaa tacgataaaa caacttgatg cttatttgga ttcaggagta
      361 acatatatta gaagcttagg ttgtcccaaa tatgtggatg tacaacttaa aaatcttata
      421 gacaaagggt ctatagatgg tcctggtata gttacttcag gtccagttat atgtatgaca
      481 ggagggcatg gatattattt tggtattgaa agtgatggag tggatgaatg tagaaaatca
      541 gccagaacag ttttaaaaaa tggagtagat tgtgtaaaga taatggcaac tggaggtgta
      601 acaactgatg gagttgagcc agggtcacca caattaactt tggaggaaat gaaggctgct
      661 gtagatgaag ctataaaagc aggtaaaata actgccacac atgcacaagg aagaactggt
      721 ataaaaaatg cagtgcttgc aggaattact tctatagaac atggtgttta tttagatgat
      781 gaaataattg atttgatgct tgaaagagga acttacttag ttccaactgt ggctgcacca
      841 tattttattc taaatgcagg aaaagaaagt ggaataactg aagctactct tagaaagtgt
      901 gagattgttg ccattccaca caaagagtca ttcctaaaag catataaaaa aggaattaaa
      961 attgcagttg gttctgatgc aggaacttct tataatgaac atggaaaaac atattatgaa
     1021 atgaaactaa tgtcagatta tggtatggat aatatggata taatagttgc tgctacaaaa
     1081 acagcatctg aacttttaag aattgataaa aattatggaa ctctagaaaa aggaaagaaa
     1141 gcagatgtta tagttgtaaa tggaaatcca gtagagaata tagacgtact tgcagatgtg
     1201 ttagcagtat ttaaacttgg taaaaaagtt agataaatag tataaaaata aaaaagagtg
     1261 tctaaagaaa tttttaaata attatagaca ctctttttta ttagtaataa ttaaatcttt
     1321 tatactttag aaaatagatt ctattttata tttagatata actctttaaa atcattctta
     1381 tgcaatataa tctatagaat ctacacatac aaagtatatt cttgaatcat tagaatttcc
     1441 aagtactata acatcatttt tcttacctag aacttcaaca ccagttagaa ccaaactacc
     1501 agctactaca gtaacttgat tatttatatt tgacatataa agattatcta ggataccttt
     1561 attacaacag caatcatcac aatcgcagtt gcaattacat tctttgtaat gttttttatc
     1621 aagatagtaa cctaaagatg ctatgaaatc ttcataatct gcaggtgtaa tatcaaatgc
     1681 aacagcttta agattacaca atgatgctaa atctacgttt gctaatggag tagatgcagc
     1741 aagtataaaa tcaattatag cagcaagtat tgcatctaat atagccccaa ggtctatcgt
     1801 aggaggaata actgcatcaa taagtgcaat taattcagct actcctggta tagttcctat
     1861 agtattaatt aatccctcaa gagttaaagg gactggaata ggataagata ctctaccagc
     1921 tatatctatt aaatcacagt tacaagcaga aaatctttca aaagtaccat caagtccaga
     1981 taaattatct tttggaggag ctgaaagatc tatacctacc aaattagcac ctactataaa
     2041 gaaatctgag ataaaagcaa attgattaaa gtttacaaaa ggtcttagag catcatatct
     2101 tagaagttct aaggcctctc tcatactttt tttacaacaa cattgtttta ttttaggttc
     2161 tttatattta tttggataat tatagtatct atcagctact tgataatttt catagtatct
     2221 ttcatttgta tttggataat cttcctcata ttcttgtgta aatctaaagt cctctctaca
     2281 tttattattt tccactaaat acactccttt tatattttaa tttaaatgta tagataatta
     2341 agcttatcta tatagtaata ttatatttat aatgtaaaaa aatgttacaa aagatattat
     2401 aaacgaataa aaaataataa tttgtcagat aaacttgtaa atttttatga ataatccatt
     2461 ctaaaacaaa aaactgtgta taaaaaaggt ttatatgtta tatttatata aatataacat
     2521 atgaacgaga aaggagtggt ttgataaatg attgaggtag ataaattgtg taagtctttt
     2581 acaagggttg taaaagatga taagaataaa agttcaataa gaaagctaaa gaaagtaaaa
     2641 actaaaaaag aagaattttt tgctgtaaat aatgtttcat ttaaagttat ggaaggtgaa
     2701 attgttggaa tattaggtcc aaatggtgca ggaaagacta cattacttag aatgttaggt
     2761 ggcatattaa caccaacatc aggaagtata aatatttctg gatatgatta ttcaatagat
     2821 agaaattcag caaaaaaaga aataggttat ctctctggaa atacaaaact atatggaagg
     2881 ctttctccga gagaacttct aacaactttt gcaagtctat atgagatgtc aaaagaagat
     2941 atagaagaat caattgaaaa tattgtaaaa ataatggata tgagtgaatt tatagataat
     3001 agaattgaaa atctatcaac tggtcagact caaagaacat ctatagctcg ttgtttaata
     3061 cattctccaa aagtatatat ttttgatgag ccaacactag gtctggatgt tttaagtagt
     3121 gcatctatta tagattttat gaagaatgaa aaactaaggg gaaaaactgt tctttactca
     3181 actcattata tggaagaagc agaaacttta tgtgacaaga tttttatgat tcacaatggg
     3241 aaaataattg catcaggaac accacagagt ttaaaagagg aatctggagt aaataatttg
     3301 agagatgtat tcataaaact tgccagaaag gaggacattt aaatgagaag taagattgta
     3361 aaatacatat ttaaaaaaga aatgctagac atattgagag ataaaaagac tttatttatg
     3421 atgattgttg tgccacttct tttatatcct ttacttatgt tattaatgat tcaagtaatg
     3481 aatatgtctg caagtagtat gaatagtaag gacattaata tagcttttaa taataaacct
     3541 aataaggttt tagtatccat gattgaagat gataatttag aaaaagatac taaaaatact
     3601 tcaaatgata aaggcaaaat aagagtgtta gatgtagata actataaaaa gtcattagaa
     3661 gaaaataaaa ttgatgctta tataaagatg gaagaaaaaa aatcatctat aaattatcaa
     3721 atatatataa attcttctca agaaaattct tctaatgcac taaaaagaat tgaaactgtt
     3781 ttggataaat ataaaagatt taccatagaa aaaactttat ctgaaaaagg attagatgta
     3841 caagctactt tagaaccaat aacatatagc actgtagatg ttgcaaagac ggaagaagtg
     3901 gcaggatact ttttaggtca gatacttcca tttatattaa taataggagt tttactaggt
     3961 tcaatatatc cagcaataga tgttatggct ggagaaaagg aaagaggtac attagaaaca
     4021 ttatttacac tgccaatatc aaacttagaa ctggttatgg gaaaatatat ggcagtttct
     4081 cttagtgcaa tagtaacagc aattttaaac attttatcta tgggactgac tatggcattt
     4141 attttagtta gtggaggtat tagtagccaa tttggctttg gaaactttaa ttatggagac
     4201 ttagtcttac caattataac tactttaatt tgcatatgtc ttttttcaat ggtagtttca
     4261 gcaataagta tgtgtgtatg ttcattagca aaaagtttta aagatgctca aaactacata
     4321 acacctctta tgttaattat tatgatacca tcatatgttt ctatgatacc aaatatagta
     4381 ttagatagag tgacagctgt aatacctgta gtaaatatat ctttacttat aaaaagtgta
     4441 ttatctttta aaagcgattt aaatttaata gccttagttt tagcatcaaa tgtagccttt
     4501 gttatattgg caatcatact actgtctaag atgttcaatt ctgaggaaat actttttggt
     4561 aatagtaaga gtttttcttt cttagagaaa agaagtaata taaaaaaagg gtctatacca
     4621 actgtaagtg atggtgttat tttatatgca gtaggattag ttctattgat ttatgttggt
     4681 tctttagttc aaattaaatt tggtatggca ggtattataa tgacacaatt catgataatt
     4741 tctttaccat tattatttgc atactatatt aaagctgatt ttaaagaggt atttagcttg
     4801 acagttccta aattaaagca tgtgtttggg agcattgttc tttggattgg tggatacata
     4861 cttattatga ttataacaca gctaatttta ttcttatttc ctcaaaatat ggaagttgct
     4921 gaaagtttaa ataaggctct ttttatcaaa gatagtgttt tactgaattt actagcaata
     4981 gctttattac cagctatatg tgaagaaatg ttttttaggg gatttatatt ttcttcattt
     5041 agtaaatcaa aagataaaaa taagtctatc aagctagcta taatatgcag tggggtacta
     5101 tttggtatta tgcatatgga ttttattaga ataattccaa catcaatatt aggtattata
     5161 tttgcatata gtgtgtataa atctggttca atatttgtat caatgttatt gcatttttta
     5221 aataatagtg tagcagttgt gttaaatcat tatacaactg gaaatataac caacttatat
     5281 aaatttgttg aagttgattt ttcgaactta aatgtactta aatttgcctt aattcttcta
     5341 ataagtctta tattaattat gttaggatta agagttttag atagaaagtc tttaagaaat
     5401 taagtataaa gttattatat agattaaaat attaataagc actaaagtat atggatatat
     5461 cttgtatata aaaatacttt agtgctattt tatatatttt aattatatga attatttatc
     5521 gtttcattta tgtaatgaaa atgttatata aatgaaacaa taaataattt ttattttaga
     5581 aat
//
LOCUS       DACXMZ010000231         5501 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.232, whole genome shotgun sequence.
ACCESSION   DACXMZ010000231 DACXMZ010000000
VERSION     DACXMZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.232"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            108..2609
                     /locus_tag="JD016_16765"
     CDS             108..2609
                     /locus_tag="JD016_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAU5001272.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2769..3437
                     /locus_tag="JD016_16770"
     CDS             2769..3437
                     /locus_tag="JD016_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5001273.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3421..4698
                     /locus_tag="JD016_16775"
     CDS             3421..4698
                     /locus_tag="JD016_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAU5001274.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5021..5320
                     /locus_tag="JD016_16780"
     CDS             5021..5320
                     /locus_tag="JD016_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001275.1"
                     /translation="MILQINICTLGKINIGSIIFTEEVVLMDRLKRDMKNMKEDLKEG
                     VKKTAYVMKNTAEDLKDDAQGAMVNLEMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         2317 a          556 c          979 g         1649 t
ORIGIN      
        1 tataataaaa atatagattt ctaaataagt aatttatcaa tattacatat caaaataaaa
       61 cgttacaaat tagctgaaaa tatattgaaa gaaaggaaga ttttattatg agttcaaata
      121 aagtggtaga aagttataaa attacaggta tgacatgtgc agcttgtgca aaggcagtag
      181 agagagtaac taagaagatg gatggagtat atgaccaaag tgtaaatata gctactgaaa
      241 aacttaaaat agaatatgat aattctaagg ttaattttga tgatataaag caggttgtag
      301 aaaaagcagg atatggaatt ataaaggaag aaagtaataa aaaaattgat atgaaaattg
      361 atggaatgac atgtgcagct tgtgcaaaag ctgtagagag agtggtaaaa aaattggatg
      421 gagtagaaag tattagtgtg aatattgcaa cagataaagc taatatagac tatgacccat
      481 caaaagtaaa attatctcaa ataaaagcag ctattgaaaa agctggatat aaaccaatag
      541 aagaggtaag aaataaggtt gatgtggatg aagataaatt gagaaaagaa agagaaatga
      601 agtctttatt tgttaaattt atagttgcaa tagtatttgc agtacctctg ttttatatag
      661 caatgggacc tatgataata aagcctatag ggccatggcc tttgccagaa attataaatc
      721 ctatgacaaa tacttttaat tatgcattaa ttcagttaat tttagtaata ccagtaatga
      781 tagctggtta taaattttat ataaatgggt ttaaatcact tttttcttta agccctaata
      841 tggattcttt agttgcaata ggaacattag ccgcattttt atatagtcta tatacgacac
      901 tacaaatagc caatggacaa attcaaggta tgcaccatca tcagttgtat tatgaaagtg
      961 caggaataat aatagcactt atcttacttg gtaaatattt agaatcaaaa tcaaaaggaa
     1021 aaacatcaga agcaattaaa aagcttatgg ggctacaacc taaaactgca atagtgttag
     1081 ttgatggtaa agaagttgaa actcctatag aagaagtaga gataggagat atactattag
     1141 taaaaccagg aactaaaata cccgtggatg gagttgtaat agaaggatat acatcagttg
     1201 atgaatcaat gcttacaggt gaaagtatac cagtagaaaa gaatgttgga agtaaggtga
     1261 caggagctag tattaataaa aatggtgtta ttaagtttaa ggctgaaaaa attggtggag
     1321 atactgctct tgcccaaata ataaagttag ttgaagatgc acaaggtaca aaagcaccta
     1381 ttgctaaact tgccgatact gtatctggat attttgtacc aatagtaatt gcaatagctg
     1441 tagttgcatc attattgtgg tttttaatag gaggaaaaga tatagtattt gtacttacta
     1501 tatttatatc agtacttgta attgcttgtc cttgtgcatt aggattagca acaccaactg
     1561 ctataatggt tgggactgga aagggcgcag aaaatggaat cttgataaaa ggtggagagg
     1621 cactagaatc tgcacataaa gtaaatacag tgatatttga taaaacaggt actataacag
     1681 aaggaaagcc aaaagtaaca gatatagtat taaataataa tgtaaaagaa gaatatctta
     1741 tcaagattgc ttcaagtgca gaaaaaggtt cagagcatcc tcttggagaa gcaatagtta
     1801 agtatggaga agagaagaat ataaaatttg aaaaagttga taattttaaa gccataccag
     1861 gtgctggtat acaagttact attaatgatg aaagtatatt acttggaaat agaaagctta
     1921 tgaatgacaa taatataaag cttggggatt tagaagaaaa atcaaatata ttggcaagcc
     1981 aaggtaaaac acctatgtat atagctgtag atggaaattt atcaggtata atagcagttg
     2041 cagatgtagt caaagaaagt agtaaaaaag caatagaaat acttcatgat atgggaataa
     2101 aagttgcaat ggtaactgga gataatgcga aaactgcaaa tgctatagca aatcaagtag
     2161 gtatagatat ggttttggca gaggttttac cagaagataa gtcaaaagaa gttgaaaaac
     2221 tacaaaatca aggaaagttt gttgcaatgg taggggatgg aataaatgat gccccagctc
     2281 ttgcaaaagc agatataggg atagcaatag gaagtggaac ggatgtagcc attgaatctg
     2341 cggatatagt acttatgaag agtgatttaa ttgatgttcc aacagcaatt aaacttagtc
     2401 atgaaactat aaaaaatata aaacaaaacc tattttgggc atttggatat aatacaattg
     2461 gtatacctgt agcagcagga atattatatg tatttggagg acctttatta aacccaatga
     2521 ttgcagcagc agcaatgagc ttaagttcag tttcagtcgt atcaaatgca cttagactta
     2581 aaaacttcaa ggcatataag agagattaga ttaataaatt aaataaaatg tatccaaaag
     2641 agctatcttt aaattaaaag gattaattta caaattaaga tagctctttt ttcatgtaaa
     2701 attcacaaca ataagataaa atataagtaa tatacgaaat tttaaataaa atagaaagga
     2761 ttgatagaat gaattcgtca atacttgtaa ttgaagatga ttcaaatata caagagttaa
     2821 tatcagaatt tttaagtgca gaaggttatc aagttgatac agcaaatgat gggttggaag
     2881 gtatacaaaa gtttaagcaa ggaagttatg atttagtcat attagatata atgatgccaa
     2941 acttagatgg ttatggagtt tgtaaaatga taagaaagtc atcgagcgtt ccaattatat
     3001 ttttaactgc tttaaatgat gaaggagacc aacttaaagg gtttgactta gaatgtgatg
     3061 attatataac aaaaccattt tcatttaatc ttcttataaa aagagtagaa gcaattttaa
     3121 ggcgaagtaa caagactata aatgacaaat ttatagtttt tgaaaaatta aaattagatt
     3181 taaacacata tattgcagag atagatggag aacctataga acttacacta aaagagttta
     3241 atatattaaa agctttgata gaaaagtatc cacaagttat aactagggaa ggtcttttgg
     3301 atagtatatg gggttatgac tattatggag atactaggat tgttgatgca catataaaaa
     3361 atataagaaa aaaaatatca ttaccatata ttaaaactgt aaaaggaata ggatatacac
     3421 ttgagaagga tatttgataa gtgggaaaag ttaagtataa agtataagtt attttcaata
     3481 acgacatcat tattaatagc attagcttta ataatatatc taattttata ttttttattg
     3541 ccatcatatt atcatgagta taaaattgag tctcttcaag aaagtttgaa atctttagta
     3601 gatagttcaa ttcatttcga tacatatact ttagaagaaa gactttatta tatggctaaa
     3661 gaccaaaatt tggctatttt attaaaagat aaccaaggta aaattgtata tggtaagaat
     3721 gaagtagtta ttttaagata tagtaagtac atgattaata gtcttgagga tgagtataga
     3781 acttctatac ctatatatac taaagatgca aaagatggtc catatacttt agaactcgtt
     3841 atgccattac aaccaattga cgaagcaaac gaagttataa gaaaactaat gccatacatc
     3901 atatcaatag caatattgat agcgattatt ggagcatata tatattcaat tgtaataaca
     3961 aagcctctta taaacataat agagagtgag agagaacagg aatatagaag gaaggatttt
     4021 gttgctacaa tatcacatga actaaaaact ccaataacca taataagtgg tcaaattgaa
     4081 ggtatgattt atagtgtagg taagtataag gatagggata cttatctgaa aaaatcgtat
     4141 gagtgtacac aagaattaaa agatttagta aatgaaatga tagaggtttc aaaatcagaa
     4201 atacttgaaa aagatttgaa attggtatca ataaatataa gtgaattatt aaatagactt
     4261 gtaaagagac aagtattttt aatagaagaa aaacatatga aaactatttt aaaaatagaa
     4321 gaaaatttgg aaattaaggc agaccaagag agaataacta aggcaatcaa taatataata
     4381 aataatgcca taaaatattc tcctgaagaa tcagagctta taattagact ctatgataaa
     4441 aataaacgaa taagtaaaaa gacttctaat caaagagtgg ttttagaaat tgaaaatact
     4501 ggtgtaacta tcgagaaacg ttatttagaa gaaatattta atccatttta tagaatagaa
     4561 aaatctcgtt cgagaaaaac tggaggaagt ggacttggac tttatatagt cagccaaatt
     4621 tttaaaagtc atgggtttga ttatagcata aaaaacaaag aaaactctgt agtattcaca
     4681 gtagaattta aaaattaatt acttattata aagaatatat aactttcaat aatagttcat
     4741 tttaaaatca caaaaaattc ataatgaaac tgtattatat aaatataaag aagcttaaaa
     4801 ccataaaata caccaactat aatgatgaga tttccttaca tcctccatat aagtcatcat
     4861 tatagtaacc caacaattaa gttactatga atttaaaaaa taacaccgag catataatct
     4921 atgcttagtg ttatttttat tttgaaataa aaaatattta atttattgaa atgtatagat
     4981 ttagatagtt taaactatat ttaagactta aaaattataa ttgatattac aaataaatat
     5041 atgtactttg ggaaaaataa atataggaag tattatattt acagaggagg tagttttgat
     5101 ggatagatta aaaagagata tgaaaaatat gaaagaagat ttaaaagaag gtgttaaaaa
     5161 gacagcttat gttatgaaaa atacagctga agatttgaaa gatgatgcac aaggtgctat
     5221 ggtaaactta gaaatgaaaa aagatgagat gatggaaaga tatgaacaaa agaaattttc
     5281 taaagaatta gagaagcaaa tgaagaatga aatgaaataa gcttttattt atgaataaca
     5341 aattattgta ttcatataaa aagtagccag taatattaaa tagtttattt aaggaaatag
     5401 actttctaaa aataactgta ttagatttag aaagtctatt tcaaattagt tttataaata
     5461 gacataagga taaagtagat gttatagaac tagattttaa a
//
LOCUS       DACXMZ010000232         5428 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.233, whole genome shotgun sequence.
ACCESSION   DACXMZ010000232 DACXMZ010000000
VERSION     DACXMZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.233"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            142..1347
                     /locus_tag="JD016_16785"
     CDS             142..1347
                     /locus_tag="JD016_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAU5001276.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1418..1639)
                     /locus_tag="JD016_16790"
     CDS             complement(1418..1639)
                     /locus_tag="JD016_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001277.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(1780..2379)
                     /gene="recR"
                     /locus_tag="JD016_16795"
     CDS             complement(1780..2379)
                     /gene="recR"
                     /locus_tag="JD016_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAU5001278.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2392..2742)
                     /locus_tag="JD016_16800"
     CDS             complement(2392..2742)
                     /locus_tag="JD016_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAU5001279.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(2801..4438)
                     /gene="dnaX"
                     /locus_tag="JD016_16805"
     CDS             complement(2801..4438)
                     /gene="dnaX"
                     /locus_tag="JD016_16805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAU5001280.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4515..4779)
                     /gene="ffs"
                     /locus_tag="JD016_16810"
     ncRNA           complement(4515..4779)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JD016_16810"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4817..4908)
                     /locus_tag="JD016_16815"
     tRNA            complement(4817..4908)
                     /locus_tag="JD016_16815"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4872..4874),aa:Ser,seq:gga)
     gene            complement(4909..5364)
                     /locus_tag="JD016_16820"
     CDS             complement(4909..5364)
                     /locus_tag="JD016_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HAU5001281.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
BASE COUNT         1723 a          977 c          630 g         2098 t
ORIGIN      
        1 catttctttc acttgtaatt tttgcaatct tgtattagat taatattaaa aataatttat
       61 caataacctt attcacaaag acagttttta tatatcaaaa tataatataa atactatgct
      121 acattttagg aggattttat tatgagcaaa aaagtattaa taatgtctgc ttccactggt
      181 ggtgggcata atagggctgc tctagctatt aaagaagaac ttacttccaa aacacttgat
      241 ggagagccaa ttgaatgtga aataatagat agtttgaaat tagttaataa tacaatggat
      301 aaaataattt caagaggata cgaaaaatct gctatatata ctcccaaagc ctatggaagt
      361 gtatatagat tatctgaaac aaatcttttg tcaaaaaatg aatttaaaga taatctacta
      421 ataacattca tggctaaaaa atttaaaaag cttattagaa gcgaaaaacc agacttgatt
      481 ataggaacgc atccttttcc aatgattgct ctaagtacat tgaaaaaaaa ctttaattta
      541 cataataatg aatcaaatgc ttacacagag catttttata aacattatac aaataccatt
      601 aatgttcctc cgcttatttc tgtattaacc gattatacta ctcattctac atggatacaa
      661 aatgaaattg attactatat tgttggtcat gaatatgtaa aagaattatt ggtgtttgat
      721 ggagttgaac cgagtaaaat tagaacattt ggaatccctg ttgaaaaatc ttttctctct
      781 cacagagata aagatattgt tctttctgag ttgaatttat ctcctgataa attaactgtt
      841 ttacttatgg ggggtagctt tggggctgga aatataaaag agacacttga tgaattactg
      901 gatactgata gagactttca aatacttgta attactggta agaatgaaag cctaaaagaa
      961 aaaatcgaaa aaaaacttat gtctcgttat catgataaaa atgtatgtgt tcttggatac
     1021 actgataaga tgaatgatat tttagcttct atagatgtat tagtaagcaa acctggtgga
     1081 cttactacaa ctgaggctct tttaaaagat gtacctatga tagttcctta ttatatacca
     1141 ggtcaggaag aagagaatct tgatttctta tctaattgtg gtgcagcact tagaactaca
     1201 aaaaaatata acttgtcaat attactaaaa gttttaatag atgaccctag cagattagaa
     1261 atgctaaaaa gaaatattaa atcaataaga aagtcaaatt cagcccaaaa tatagctaat
     1321 ttaattttaa atataatttc tggttgataa aataaataaa atccctatag tatatatatt
     1381 atctattagg gattttttat tttctaatta tttaatatta aattggttca taaaagtctt
     1441 cttcataatc atactgataa tttttataat tatttctaaa ttcagcattt ttatttcgta
     1501 catttttttt agtttttttc ttattagaaa aacttatatc ctcaaaatca tcatatttag
     1561 gtttatcttt tgatttcatt tttttaactc ttttttcttt cattgcatta tgttcttcta
     1621 agttcataat tggtttcatg ttgcacccct atcaaaattt ttaaatgatt tatacaacaa
     1681 attatacaat acagaagaaa tttattcaat aaatctaaat caaaattaat ttgaatttaa
     1741 ctcaaaaaat tacaaaaatc gaggtattaa cctcgatttt tatatttctc ttctgccttc
     1801 tagtgctttt gatattgtga cctcatctgc atactctaaa tcgcctccaa caggaagacc
     1861 atgagctatc cgagtgactt tgattcccat tggttttact aacctagcaa tatacatggc
     1921 tgtcgcttca ccttcaatag taggatttgt tgccattatt atttctttga cttcctgatt
     1981 gccaagtctt tggataagtt cctttatttt taacatatct ggtccaattc catccattgg
     2041 agaaattact ccatttaata catgatactg ccctttaaac tctctcgttc tctccatagc
     2101 tgctacatct cttggatctt ccacaacaca tataacactt gaatctctac ttttatttga
     2161 acacatgcta catgggtctg tatctgttat attacaacaa attgagcaat atcttatttc
     2221 tctttttgca tctataatcg ctttagataa agcttccaca tcattagtgt tcatattaat
     2281 aatatgaaaa gccaatcttt gagcagtctt tcttcctact ccaggaagct ttgaaaattc
     2341 ctctataagt ctcgttatgg gacctgtata aacttgcata aataccacct actagaataa
     2401 cccaggtata ttcatacctc cagttacctt actcatttga cttgcttgta tatcatcaat
     2461 atttctcaat gcttgattta ctgcacttaa aactaaatct tgtagcattt ctatatcatc
     2521 tggatcaact acttctggct tgatagttat atctataact tcttttttac cattaacttt
     2581 tacagtaact gctcctccac caactgaagc ttctacttct ttactttcta actcttgttg
     2641 agctttttgc atattttctt gcattttttg agcctgcttt aacaagttgt tcatatttcc
     2701 accaccaggc atcattccgc ctccaaaacc ttttttagcc ataaacacta ttcctcctaa
     2761 acttattttc attttattat tatatcataa tagtatttta ttatttactc tcattttcat
     2821 caatactatc ttttatttct aatatctctt cactcactat gttctttaat atttcttcac
     2881 caatgtcctt tttctcttta acttcaagat ttatatcttt aacttctgat gtcagtacta
     2941 tttttacatt aaaacttcta tttaaaacat ctcttattgt tttttctaaa taatttattg
     3001 tatcttgaga gctcagtcta gtttttgcaa aatcaaattt atcatcaaat ataacataca
     3061 acatattaga atatacatta aaacttttta cttcacttaa gagtgcgtat attggcattt
     3121 ttttatcttt tttaatttgt ttaagtatat tcttccaaga tgactctata agtataacgt
     3181 cctcattttt tacatcttca taaactacat tttcttgttt tacatctatt tctctatcga
     3241 cctcatactc tttttcttta ttattcccaa ttttttcaga cttaaagttc cccagttcaa
     3301 tcattttttc taagttctca actcttttta taagagcttc tttactttca tcaaacattg
     3361 gttgagcaat tttcattatg gttatttcca ctaaaaccct tggatttgaa gatgacttta
     3421 tatcatcctg agttattgat aatatattta aaatccttat taaatcattt atatctatat
     3481 ttttagactg aattttaagt tgttctatag tttcttctgg taatgaaatt atttcatcca
     3541 attctgatga aaccttacat accattaaat ttctaaaatg atcaattaag tcatttataa
     3601 gatttcttat atctttgccc cataagacaa attcatttaa tatctcaaga gactttttag
     3661 tgtcctgctc tacaatacat tgagacaatt caaataattg ttctatattt acgcttccca
     3721 ttaattcaac aacatcttta taatctatct tactttctcc aaaagacata cattggtcta
     3781 atatactaag tgcatctcta agagctcctt gagaatttct agctattaaa ttaagagctt
     3841 tatcatctat atcaattcct tcatcttcac aaatcttttt cattctaagt gtcatatctt
     3901 taactgtaac tcttttaaaa tcgtatcttt ggcatcttga taaaatggtt gcaggtattt
     3961 tgtgaggctc tgtagttgct aatataaaaa ttacatatga tggaggttcc tctaaggtct
     4021 ttaataatgc attaaaagct ccttgagaca acatatgaac ttcatctatt atatatactt
     4081 tatatttagc attggctgga gaatatttca cactttctct aagctctctt atatcatcta
     4141 cactattatt tgaagctgca tctatttcta caacatccat tgtattatct tttaatacag
     4201 attcgcatat ctcacactca ttacacggtt cttcatttat tttattcaaa cagtttactg
     4261 ctcttgaaaa aatctttgca gtagatgttt taccagtacc tcttgttcca caaaacaaat
     4321 aagcatgtcc tatattatca ctatatattt gattctttaa agtcttaata atatgatctt
     4381 gaccaataac atcttcaaat ttttgtggtc tatatgctct atataatgct ttatgcatct
     4441 ttttacctct ctttagcaaa attaataaaa aaatccctca ttatctaagg gattagtctt
     4501 ttatatatta aatgctgtac accatctatc gacttaattc cataggcgtt accaaaacag
     4561 ttagctcaac ttaggcaccc ccacggcaca caaaagtgat cacttaccgc tgcttccttc
     4621 cggatctgac ggggttcatg agctttcatt gcgcaggacc taagcatcaa caccacttat
     4681 ttagggcggc cctacagaaa ataagccttt agatggaatt caatcctgct atagcggatt
     4741 gcaggttaca gggcaccgct acctccccat ctagtacagc aaaattacaa ccatatttaa
     4801 ttactttcat tgtttctggc ggagagagtg ggatttgaac ccacgataca gttttaccca
     4861 tatactcgct ttccaggcga gcgccttcga ccactcgacc atctctcctt aatctctttt
     4921 tctcaatgcc ttaaaaaatt cttgcaatat gatcgagcat tcttcttgca aaacatcaaa
     4981 agctactttt acattattat tattaaaata atctaattta aactcatgct gtttttctat
     5041 atatgaattt tttacatgtc ttgtccctat tataagcttt tttattctag aattaactat
     5101 tgcgccacta cacattatgc aaggttccat tgtaacatat aaatcacaat cagtcaacct
     5161 ccaaccacct aatttttctg atgcttgttt tatcgctaaa atttctgcat gtgccgtaga
     5221 gtcttttaac gtttctgtaa ggttatgagc tctagctatt atttgattat ctttaactat
     5281 aatagcacct attggtgttt cttttttatt atatgcttta tatgcttctt ttaatgcttc
     5341 tttcatataa aatgaacttt ccataattcc tcccaaaact tataacaaac ttatcttatc
     5401 ataattcatt ttataattca acttaatt
//
LOCUS       DACXMZ010000233         5400 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.234, whole genome shotgun sequence.
ACCESSION   DACXMZ010000233 DACXMZ010000000
VERSION     DACXMZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.234"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(292..474)
                     /locus_tag="JD016_16825"
     CDS             complement(292..474)
                     /locus_tag="JD016_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001282.1"
                     /translation="MITKDNTLKCEDFNIQYCALNNITPKYHPPFTIIEYPNYFKKEN
                     IRNGKPCIYKIKNTNF"
     gene            complement(742..870)
                     /locus_tag="JD016_16830"
     CDS             complement(742..870)
                     /locus_tag="JD016_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001283.1"
                     /translation="MNEILEIVNSHKHFLKLHKIGCIKYLLKVRIEYIKEKVILYE"
     gene            987..1361
                     /locus_tag="JD016_16835"
     CDS             987..1361
                     /locus_tag="JD016_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU5001284.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYEED"
     gene            1363..2220
                     /locus_tag="JD016_16840"
     CDS             1363..2220
                     /locus_tag="JD016_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001285.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            2223..2861
                     /locus_tag="JD016_16845"
     CDS             2223..2861
                     /locus_tag="JD016_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAU5001286.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            3872..4606
                     /locus_tag="JD016_16850"
     CDS             3872..4606
                     /locus_tag="JD016_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAU5001287.1"
                     /translation="MNLAKGLDVLKISSNVLGEDKVMYIPAIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIETLKNLIIE"
BASE COUNT         2080 a          568 c          795 g         1957 t
ORIGIN      
        1 ctaatagtta taatttgttt ttatttgcta aaatgcaact ttaatttgat ttatgtataa
       61 tatagggtat ttaataatat ttttgaagaa tttttataaa aaattaaaaa gaggagaaaa
      121 aagggagtgc cacaaagtga actttttagt tctaaggcac tcttttttgt acgaaatcga
      181 atatattttc aacgttttaa aagtgagaaa gagacttatc tctagtttga gatagccaat
      241 tttaattatt aaaattattt tttgttttat ttatcaaaga gtattcgtgt tttagaagtt
      301 agtatttttt attttatata tacaaggttt tccgtttcta atgttttctt ttttaaaata
      361 attagggtat tcaattatag taaaaggtgg atggtatttt ggagtgatat tatttaaagc
      421 acagtattga atgttgaaat cctcacattt tagagtatta tcttttgtaa tcaaaaaaat
      481 cttctatact tatagcaagg ttacttctgc aaatattcca tacataataa tatcccaatt
      541 taaatttttt taatatagag tttacctcta gtctacactc ttgaataact ctttcctcta
      601 tccaaattct tataaattca tcccatttat tactatttat ttattattat ctaaattttt
      661 tagtatgttt gtaattagaa atggatttat tcctttatgt tttttataaa tagtttataa
      721 cttaatttat cattaaacaa atcattcata caggattact ttttctttta tatattcaat
      781 tcttactttt aataaatatt taatacatcc tatcttatgt agttttaaaa agtgtttatg
      841 gctattaaca atctctaata tttcattcat atttattttg tcatagtaat attttacatc
      901 ttttatattg acaaaaaagt gctctaagtg tatagtgtgt atatgataac atcacactga
      961 aagtgataaa aagggggtga taaaaaatga aagtgctcat ttctaacaca tcagataagc
     1021 cattatacca acaaattaaa gagcaaatta aagaagcaat atttactgaa gaactgaaag
     1081 aaggggatgc aatgccatcc ataagaaatt ttgctaatga tttaaaagtt agtattttga
     1141 ctattagacg agtgtatgaa gaacttgaaa atgaaggctt tcttacacgc caagttggaa
     1201 taggaacatt tatttcaact ggaaatttag aaatactaag agactcaaag agacgtatag
     1261 ttgaacaaaa aatggcagat atgattaaag atgcaaaagc actaggaatt acacagatag
     1321 agttacaaga aatgatggat attctatatg aggaggatta atatggaaaa tattttagaa
     1381 gtttataact taaataagac ttataccgat ttttcattat ctaatataag ctttgcatta
     1441 ccagaagatt gtataaccgg atttataggt gttaatggag caggaaaaac aacaacaatc
     1501 cgttcgatat tggatgtcgt aaagcgtgat agtggaaaaa taaaaatctt tggtaaagat
     1561 ttcgatggaa atgagaagga attaaaaaat cgaattggag ttgtctttga tgattcatat
     1621 ttctatgagg agttgtcaat ggcagaaatg aaaagtatta ttgcaccttc atatgataat
     1681 tggtgtgaag aagattatca acaaaatatg aagcgtttta atcttaaccc aaaacaaaag
     1741 attgatacac tttcaaaagg aatgaaaatg aaatttgctt tagcattagc attatcgcat
     1801 aaagctgagt ttttaattat ggatgagcct acaagtggtt tagacccact aatccgtagt
     1861 cagatacttg acatactaac aggatatatg aagaaatgtg gtcgtggtgt tttcttttcc
     1921 actcatataa catctgattt agacaaaata gcagatatga ttgttatgat tgacaatgga
     1981 cgtattatat tagagcgtga aaaagatact ctattggacg aacatagaaa agtaaaaggg
     2041 gatattcaat ttctaaatga tgaaaccaaa aaattatttt taaatttaga aattacaggt
     2101 tatggtttta tagggattac ctcaaaaatt aatgaagtgc aaaaaaatat gccagatatt
     2161 ttaatagagc gtcctacaat tgaagatatt atgcttgcat atattgaaag gagagagtaa
     2221 ttatgttgat tcatttagtt aaaaaagata ttctgattgt aaaaaaatat gtattgttga
     2281 tattatttat tgcaatagga atcccattat ttatattatg gcgtttacct ccatttgcac
     2341 aaatattggg atttgtacta tcaattatat tttcagaatt tatgctgtgt cagtatctat
     2401 tattaaagga gagtcaatat tcaaaagcat ctgtattact ttgttcaact ccttatccta
     2461 gacgtggctt agttcaatct aagtatgttt tatttgcaat ggtatttatc tattgtacat
     2521 tagtttactg gattgaaaat ttacttattc cacaaatagg aacttttgac ttaacatata
     2581 ttttgattgt tttattaata agttctatta tttatggagt ttatatgcct attcaataca
     2641 aattaggata tgaaacaaca aaattctttt tcgtggtgat aatcatggct tcatcatttg
     2701 tattaccatt aactatagct cacagcaatt ttgaaattcc atttatatat cagtatccat
     2761 ccatgatagt gaatattgta ttgttccttt taagtttaat tatactgtta gcttcaatgt
     2821 ttatttctat taaaattttt agtaataagg agcttgtata aggtaagggg gacttttata
     2881 tgccacattt accagattgg gctgttttac tttgtatctt acttccaata gtatataaag
     2941 cttgtcagat atttaaaagt aaaaaataga gtacatattt atattgccat ttttattgag
     3001 tgaagttaga tggtatgaat ttaaaaggtt acttgaaaat aaagttactt ggtgcgggag
     3061 agtaacatca taatattata gattatatgt ttcttgtgaa tcaatttgta ttttttaata
     3121 gggaatatcc agtaattttg ttttccattg attatttttc aattatgagt atgattgtgt
     3181 gtattgtata ccatctttta aggtgttttt aaaaaattat atcgtcaaaa tataaatcta
     3241 aggaatccta taaataaagc tttttaattt tagaaaacta ataaaaagta ggaagttaat
     3301 aaaaagaagc tgtctcaaaa tagagataag cttctttttc attgctgaaa tgacgaaaat
     3361 ataattgatt tcatataaaa aagagtgctt tatgaactat ttattttgaa cattagatac
     3421 acttgcactc aatttagtta ctcctaagct ttatgaacta aaagtttatt ttgaagctta
     3481 tttttattaa aagagatttt atttttctaa ataatcatct atatacagta ttgcgtttct
     3541 tttatttatt cataatttat tttcttatta aagtttttct atattcatat tttccatatt
     3601 actttacttt taattttata cgaattatca aaaataaaat agttattaga aaaaaataaa
     3661 agtaattgca aagcttgcaa ttacttactt ctatattaat tactatacca taaaataaca
     3721 aaaaaatcaa gtcgagtaat ccttcaactt aagtgatata ctaggtatta ataattttta
     3781 attaataaaa tataactaga ggaaaatcta cattttctca atttctttaa ttaaattgac
     3841 ctctaattaa gtaattataa ggagaaaaat tatgaattta gcaaagggat tagatgtttt
     3901 aaaaatttca tcaaatgtat taggtgaaga taaagttatg tatattcctg caatctacac
     3961 agaagatgat gcaacactta ttgacacagg tcttccgggt caaggtgatt taataattga
     4021 tgctcttaat aaaagtaaca cgagctttga taggttaaaa aatataataa ttactcatca
     4081 tgatatagac catataggaa acataaatta tctaagagaa aaatctaaga ataatatcaa
     4141 agtatatgca tacaaaagtg aaatcagcta tataacagga gaagaaactc cttttaaact
     4201 ttatatgttg gaacaaatgg tagataaaat tgatgataag atgcttagta tgctaaatgt
     4261 tatgggactt ggatttaaat catcatatac taaagtagat gtgtcattag ataaccatga
     4321 aaaattgaat ctaggtgagg aaatagaagt tatacataca ggaggtcaca ctagaggtca
     4381 catatgtctt tatttaaaag agtctaaagt acttattgct ggagatttgt tacaggtaga
     4441 aaatggagaa cttaagcctg ttgatgtaat gcacagcaac aaacaagagt taaaagatgc
     4501 tattaaaaat ataagtaatt atgatataga aacgatagtt tttagtcatg gtggattata
     4561 tcaaagaaac ataattgaaa cattgaaaaa cctaattatt gagtagtact atatgattta
     4621 caataatttc ttaaattact taaaattata tctaaagatg tagaatcaaa attaaatata
     4681 agtaataaga attttgcttt aaaattatac ttaagtgtta agggtgatac gtagtattac
     4741 aagtatttta gaatatagtt ttttatagta attttaaggt tctacaatac ttcagttgaa
     4801 aaatatttaa cagcatagta taatgggaaa gaaaatattt taaataaact ttagtataca
     4861 aagtgagtaa aaatatttaa atcatatatc ataatgcaga gtgaggtaaa atatttaagt
     4921 tatatatcat aatacagagt gaagaaaaat atttagataa tgtaccataa tacaaagtga
     4981 gtaaaaatat ttaaagcatg tatcataatg caaagcgaag aaaaatattt aaataatgta
     5041 ccataatgca gagtgagcaa aaatatttaa ataatgtgtc ataatacaaa gcgaataaga
     5101 ttatttaaag catgtgttat aatacaaagc gaataagatt atttaaagca tgtgttataa
     5161 tacaaagcga ataagattat ttaaagcatg tattataata taaagtgagt aaaaatattt
     5221 aaatcatgta tcataataca aagtaaagaa gattatttaa accatatatc ataatgcaaa
     5281 gtgaagaaaa atgtttaaat tatgtatcat aatataagca gagtgaagaa aaatatttaa
     5341 ataatatatc ataatgtaga gtgagtaaaa atatttaaat catgtatcat aatgcaaagt
//
LOCUS       DACXMZ010000234         5386 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.235, whole genome shotgun sequence.
ACCESSION   DACXMZ010000234 DACXMZ010000000
VERSION     DACXMZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.235"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            28..2259
                     /gene="pflB"
                     /locus_tag="JD016_16855"
     CDS             28..2259
                     /gene="pflB"
                     /locus_tag="JD016_16855"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAU5001288.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            2419..3153
                     /gene="pflA"
                     /locus_tag="JD016_16860"
     CDS             2419..3153
                     /gene="pflA"
                     /locus_tag="JD016_16860"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAU5001289.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3275..4945)
                     /locus_tag="JD016_16865"
     CDS             complement(3275..4945)
                     /locus_tag="JD016_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAU5001290.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKYQLSK"
BASE COUNT         1906 a          791 c          796 g         1893 t
ORIGIN      
        1 catttaaatc taaggaggaa atacttaatg aatgcatggc aaggatttaa aacaggaaga
       61 tggactaaag aaataaatgt tagagagttt atacaattaa actattctcc atatgaggga
      121 aatgactctt tcttagctgg tgccactgaa aatactaaaa aattatggga cgaagcaatg
      181 gtcctattta aaaaagaaag agaaaatggt ggtactttag atgttgatac aaaaactgtt
      241 tctggaatag ctgcatatgc tccaggatat ttagataaag aattagaaac aatagttggg
      301 ttacaaacgg atgccccttt aaaaagagct gtcatgccat atggtggtat aaagatggtt
      361 gaaaattcat gcgaagcttt tggatatgaa ttagacccag aaataaaaga tatatttact
      421 aaatacagaa aaacacacaa ccaaggagtt tttgacgttt atactccaga aatgagagct
      481 gctagaaaat ctggtataat aacaggtctt ccagatgctt atggtagagg tagaataatt
      541 ggtgactaca gaagagttgc tctatatggt gttgatgctt taatagaaga taaaaatgag
      601 caaaaaaaat cattagaagt ttcttgcatg gatgaggaag ttatcagatt aagagaagaa
      661 ataactgagc aaatatcagc tttaaatgaa cttaaaaaaa tggctgaatc ttatggattt
      721 gatatctcaa aaccagcaac taactcaaaa gaagctgtac aatggttata ctttggttat
      781 ttaggtgctg ttaaagacca aaatggtgct gcaatgtctt taggtagaac atctactttc
      841 ttagatatat actttgaaag agatttaaaa gctggaatcg taacagaaga agaattacaa
      901 gagtatatgg accattttgt tatgaaatta agaatggtta agttcctaag aactcctgat
      961 tacaataacc tattctctgg agacccaact tgggtaactg agtgtatagg aggtatggga
     1021 atagatggta gaacattagt tactaaaaac tcatttagaa tgttaaatac tctatataca
     1081 ttagggcctt ctccagaacc aaacttaaca gttctatggt caactaaatt acctcaagga
     1141 ttcaaagatt tctgttctaa agtatctata gatacaagtt cagtacagta tgaaaatgat
     1201 gatttaatga gagcatactg gggagatgac tatggtatag cttgttgtgt atctgcaatg
     1261 agaattggta aacaaatgca gttctttgga gctagagtta acttagctaa aactttatta
     1321 tacgctataa atggtggtgt agatgagaaa tctggtgttc aagttggacc aagatttgaa
     1381 cctataactt ctgagtactt agattatgat gaagttatga gtaaatttga accattcact
     1441 gattggttag caacattata tgtaaatact ttaaatgtaa tacattatat gcatgataaa
     1501 tattcttatg aagcattaga aatggcatta catgatagag atatatttag aactatggct
     1561 tgtggtatgg ctggattatc agtttgtgca gactcattat ctgctataaa acatgctaag
     1621 gttaaaacta ttagaaatga acaaggaata gctgttgatt ttgaaataga aggagattat
     1681 ccaaaatacg gaaataatga tgatagagtt gatagtatag ctgtagagtt agttgaaagc
     1741 tttatgaata aaataagaaa gaataaaact tatagaaatt cttatccaac tcaatctata
     1801 cttacaataa cttcaaatgt tgtttatggt aagaaaactg gtaatacacc agatggtaga
     1861 agagctggtg ctccatttgc tcctggtgct aacccaatgc atggtagaga tactaatggt
     1921 gctttagctt cattatcttc agttgctaaa ttaccatatg aacatgctca agatggtata
     1981 tctaatactt tctctatagt acctgctgct ttaggaaaag atatgactga aagaataaac
     2041 aatctttctg caatgatgga tggatacttc gctcaaaatg ctcatcactt aaatgtaaat
     2101 gtatttgata gagctacttt agaagatgct atggaacatc cagaagaata tccacaatta
     2161 actataagag tatctggata tgcagttaac ttcataaaat taactaaaga gcaacaatta
     2221 gatgttataa acagaacatt ccacggtaaa atggcttagt ctttaaattt ttatacttta
     2281 actaagatta ctactgtgta aaaaataaaa ataaaaggct atcttatatt tgaattattt
     2341 ctggtataag atggcctttt ttagagaaat attataacta ttgataaggt tattaaaata
     2401 tattgtgagg tgaactctat gattaaggga aagatacatt ctatagaaac gtttggtact
     2461 gtagatggac caggaataag atatatatta ttttttcagg ggtgtcctct tagatgcaaa
     2521 tactgtcata acagggatac atgggatatt aaatcaggaa aagaatacac agttgatgaa
     2581 ataataacgg atgctcttaa gtatacttct tttatgaagt tttctggtgg aggtataact
     2641 gcctctggtg gtgaatccac tcttcaacca gaatttttaa gtgaattatt taaaaaagca
     2701 aaagaaaatg atatacacac ttgcttggat acatctggat ttgtagatat agaaactata
     2761 gacccagttt tagataatac tgatttggtt ttacttgatt taaaacatat ggttgaggaa
     2821 aaatctattg atttgactgg tgttggtatg gataaggcat taaaacttgc tagacactta
     2881 gaatcaagaa atattcctgt atggataaga catgttttag ttcctggcat tactgatgat
     2941 atagataatt tagaaaaact aggtcaattt gtagctacat tgaaaaatgt agaaagattt
     3001 gaattgctac cataccattc tatgggaata cacaaatggg aaagtttagg tatcgattat
     3061 gaactaaaag atgttcctga tgccactaag gaagatgtcc aaaaagctag tgaaataatc
     3121 tctaaatttg gagtgaaagt atataatagt tagtgtagta tattaatcct ttatagaaga
     3181 cataacaaaa tagggtaaag tgtaaatttt taataaactt caaaacttcc actttgccct
     3241 atttttatat actatatatc tcattttata tacatcactt tgatagctga tatttataaa
     3301 attcagaggc acttacaggt cttccaaata aaaaaccctg aataatatct acacctaagc
     3361 tttttactat atcatactct tcataagact ctattccctc aacacaaact tttaaattta
     3421 taatatgagc taatttaata atatattcaa gaaaagtata ttcataacta ttatatgtaa
     3481 tattcttaac aaaacttctt tcaattttta ttatattaac agacagttct ttaaggtaat
     3541 ttaaagatga atatcctgta ccaaagtcat ctattgcaat atatactcct atacccttta
     3601 aactaataaa tttatcattt agtaaattta tatctggtat ccaacaattc tctgttaatt
     3661 ccaaaattaa aaattcaggt cttaattgat actctactaa acattctact ataaaatctc
     3721 taaaaaaatc ttcctttaac tgaatatacg atacatttac agatatttta aagtctggat
     3781 ttattttatg ccattcttta cactgtttta cagcttcttt tataatccac ttcccaactg
     3841 gtataattaa attactctgt tctagtattg gtataaattc aacaggagat acttctccat
     3901 aagtactcga atgccatctt aaaagtacct ctgcccctat tacctctttt gtaacagcat
     3961 ttacctgggg ttgaaaaaac agctcaaagt cattaaaatt attttcaacg cactctctta
     4021 gtttttgttg cattgaaatt acctttagtt tattttcata gagctcttgt gaaaaaaact
     4081 taattctatt tttaccacta atttttgcta tatccaatgc aatatcagca tgtttaaata
     4141 aatctaaata attatctcca tcttctggat acatagcaac gcctgcagtc actgtgcaat
     4201 agtacttatt tgattcaatt tcatgctgag tatttgtata catctgtatc ttttcatata
     4261 ttttctctat agtttcttta gtacacatag ggtaaaaaaa tgcaaattca tctccatcta
     4321 acctatacaa tcttacatct tttggaagat attctaatac ttcagtagca attgcacgca
     4381 atactttgtc accataagaa tgtccatatt tttcatttat atttttaaaa ttatcaatat
     4441 ccataataaa catagcacca tttttataaa ttttctcttt tataagataa ttcattctat
     4501 gttcaaattg ttctctattc cacgttcctg tgatagaatc aaatttattt ttttcaccaa
     4561 tattctttat tctcccgact agaaatatag tttttgggtc atccgacaca taaactttac
     4621 ctctacaaga aatccatact atttcttcat atttattaat cagcctatat tctaaattgt
     4681 gctcaccttt ttttccatgt agtaattctt gtatatcatc tttccatatt tgaacatcat
     4741 ccggataaac aattttactc caacaattga ccaaatcaga ttctatctct ttacttaagt
     4801 taaattcatc aaaaattgct gaagaaattt taaatttatt cctatttata tcccaaaaaa
     4861 atatataatc ttctgtactt tgagataaaa gttcaaagaa aatttctata tggtctaaac
     4921 ttaaagagtt cgattcttct ctcatctttt ccccctacaa tattactatt agtgtagttt
     4981 aatcaacaaa atatccttat ctagattata ctattttact aattttttgt ctcatcaaac
     5041 acatttcccc aactttagtt atcttatcaa atctcttact cttaactaag ttttttgaga
     5101 ttcataaagt aatttttcta taatcattac tggtgattta tttttacctg ctaaaacacc
     5161 atgttaaatc atttattaaa tcattttctc ttaaaattct atttttcgtt atataaagag
     5221 ttatcagttc tttattctat aaaacttttc tattactctt gaatttattt tatttttttc
     5281 tccgcctatt ctctttggat ttggtattgt ttttctatag attttggcaa agaagtgtct
     5341 cttcctataa tataagattt tgatatttct tcatcatcta taaatt
//
LOCUS       DACXMZ010000235         5383 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.236, whole genome shotgun sequence.
ACCESSION   DACXMZ010000235 DACXMZ010000000
VERSION     DACXMZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.236"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            180..1016
                     /locus_tag="JD016_16870"
     CDS             180..1016
                     /locus_tag="JD016_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAU5001291.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1369..2946
                     /locus_tag="JD016_16875"
     CDS             1369..2946
                     /locus_tag="JD016_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAU5001292.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2939..4123
                     /locus_tag="JD016_16880"
     CDS             2939..4123
                     /locus_tag="JD016_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAU5001293.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPIGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEESEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     NL"
     gene            4141..4740
                     /locus_tag="JD016_16885"
     CDS             4141..4740
                     /locus_tag="JD016_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAU5001294.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4764..5234
                     /locus_tag="JD016_16890"
     CDS             4764..5234
                     /locus_tag="JD016_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAU5001295.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         2018 a          561 c         1028 g         1776 t
ORIGIN      
        1 atgaaaatat gataaaatat gacaaattaa ttgatgacaa taaaaaatat tttgttattg
       61 tagtattata gggtaaattg caaaatatat acgaaagtaa aattttaaat attgacatta
      121 aattaaatat actatactat gtaagtgtga tgagagaatt tttttagagg ggatttatag
      181 tgaatataaa aaagatattt aataacaatg tagtagtaag ctccctagaa gatggcacag
      241 aaataattgt cactggtgct ggagtaggat ttaagaaaaa agtaggcgat ttgatagatg
      301 aaaatttgat ttctaagaaa tattttgttc aagacgatca aagggacaaa tacaatcaaa
      361 tacttaataa gacctctatt gaatacttta aaatttcaga agaaattata gaaaaggcta
      421 atgaagtttt aaatactcaa gtgaatgatt caataatatt agccttaact agtcacatag
      481 aatttgctgt ccaaagagaa aaacaaggta taaaattacc aaacttaata ttgaatgaga
      541 caaaacaact ttatagggaa gaatttgaat ttggattatg ggcaattgat gaaattgaaa
      601 aaaggatagg aataaaattg cctgaagatg aagcaggata tattgctatt cacataatca
      661 atggtttgga aaattctcaa aaagatgaag gtattaatat actagagttt tcaaaacaag
      721 ttattcagat aattgaagaa gtacatggat ttaaattgga tgtaaattca ttaaattata
      781 ctagacttat aactcactta aagttttttg ttcaaaggat tctccgaaaa gaaacttaca
      841 atgatagtga tattgaagat atgtataagt tagtgaataa aaattacaat aaacctaaag
      901 tgtgtactga agaaattgca gcattggtat tagacagatt tgaatataag attagtaaag
      961 aagaagaatt atatttaatg attcacataa ataaaataac aaacagtggt tgttaggatt
     1021 gtaactgata aagcaggcaa aacctaagtc attgagctta actattagat taagtttttt
     1081 gatttaggtt tttttattag atacaatcaa aatacagaca gtgactttta ggattgtaac
     1141 tggttaagca ggcaaaacct aaatcattga atttaactat taggttaagt tttttgattt
     1201 aggttttttt attagataca atcaaataca gacagtgact tttaggattg taactggttg
     1261 agcaggcaaa acctaaatca ttaaacttaa ttattaaatt aagttttttg atttaggttt
     1321 ttctataaat ttgattatgt ttttagataa aaataggagg ggaaaattat gaaaaaaaat
     1381 aagttaggtt tatggaactt cttccaaatg ttaggaaaaa cgtttatgtt cccaatagca
     1441 ctacttagtg tttctgggat gatgctagga cttggggcag gttttacaga ccctaagatg
     1501 attgaaatgg ttccattttt agggaatgaa tatgttaact tagttttaaa cttcatgtta
     1561 acaataggat tatttgcatt taacaacttg ggagcattat ttgcaatggc aattccacta
     1621 ggattattaa aaaaagaaaa agagtttggt gctttttcag gattagtagg atttatagca
     1681 atgcatatag gtacaaattt ttatttaaca agagctgatt tgttagtacc agcagaacag
     1741 atgtcaacta atggtcaagc tatgattatg ggtatacaaa catataatac aagtgtttta
     1801 ggtggtattg tagcaggatt aattgtttat gcaatatatg ataaagttag taatttaaag
     1861 ataccagaat cattaggatt ctacagtgga ccaagattag ttccaattgt tagtttaata
     1921 gttatgagta tagttggatt attaatacct attgtttggc caccattttt caatgcattc
     1981 caaagtttag gaagatggat ttcttcagca ggaccattag gatatttttc atatgcagtg
     2041 gcagaaagag ttacaattcc atttggacta aatcatcttg taacatctgt attccgtttt
     2101 acaccaatag gtggaactgc tgttattgat ggagaaacgt actttggtac attgaacatg
     2161 tttatggcat atgtagaaaa taatcagatt atacctcttg atttagctgg taaaatggaa
     2221 caaggtaaat tgatgattca atatggatta gctggtgctg cattggctat ctaccgtaca
     2281 gcaaaaccag aaaatagaaa ggcacttaaa ggacttttaa tatcaggagt gttgacagtt
     2341 attataggtg gtataagtga accaatagag tttctattct tattcatatc accagcatta
     2401 tttgctttcc atacatttat gaatggtcta gcaaacatgg tacttccata cttaggcgtg
     2461 ttgatgggat tcacaggaga tttaattgca tttataagtt ttggtgtgtt aagaggaaca
     2521 gctacaggat ggccaatagc agtattagta gcagttttat attttgcaat atactatttt
     2581 gtatttaagt gggcaattat taagtttaat ataaagaccc caggacgtga ggataaagaa
     2641 ataaaagtat ctgaaggaaa tggttcagat ttcaaaaatt tgtctacata taaaggacaa
     2701 caaatgatat ctgctttagg tggtcaatct aatatattat cactagataa ttgtgtaact
     2761 agattacgtt taaaactaca agatgtatca ttaataaatg aggatgcaat taaagaggct
     2821 ggtggtatag cagttgttaa attagatgag catacaatac aagtaattat aggaactcaa
     2881 gtgtattctt taagaaagca gatggataaa gctatggaag gtcatttgga ggcatgtaat
     2941 gattaatttt gataaaaaag tagaccgatt gggtacatat tgtacccagt gggactatgt
     3001 agaagataga tttggtaaga aggatttgct tccttttaca atttcagata tggatttaga
     3061 gtttccattt gaaataacag aggtgttgca ggaaaggtta aatcatagag ttttgggata
     3121 cagtagatgg aaacatgatg attttagaaa ttcagttacg aattggtata ttaaaagatt
     3181 taattgtagc gttgatagta actggatata ttatagtcca agtgtaatgt attcaattag
     3241 caaacttatt gagatgttaa gtaatgaagg agatggaatc cttataaata ctcctgcata
     3301 caatgctttt tatgatgtga taacaaataa caatcgtaat attataaagt cacatcttgt
     3361 aaataggaat ggattctatt caattgattt taatgatttt gagaaaaagt gtaaaaaatc
     3421 taagatattt attttatgta atcctcacaa tccaattggt agagtatgga gtgagttaga
     3481 acttgttaag atgataggca tttgtaagaa taatgatgtc aagataatat cagatgatat
     3541 acacatggat attgtatatg acaatagaat gacacctatc ttaaatgttg caaaggacta
     3601 tttagattct gtattcattt gtacatcagc gtctaaatct ttcaatatac cagcattgac
     3661 tgggtcatat gtgattatac caaatgaagg tattagaaat aaatttgaaa atatcactag
     3721 ataccgtgat tttgtaaatt cgccagcaat tctagcaata ttagccacaa tggtctgtta
     3781 tgatgactgc gaatattggt tagatgaact attaaaatac cttaaagaga atcttaaata
     3841 tacgattgat tatataaaag ataatttaga accattgaaa ttggtcatgc cagaaggctg
     3901 ttactttgct tggatagatt tttccgaact taaaataagt ggtgagaaat tccaaaaatt
     3961 attgatggat attggagaag ttgctattat gtcggggtct gtatatggtg aagaaagtga
     4021 gttttattta aggttaaatg tggcatgttc aagagaaaaa cttgaagatg gtttaaatag
     4081 gattaaaaaa actatggatt atattgcaaa aagaaattta tagtaggaag ggagatagtt
     4141 atgggatatt taaaaacaat ggttgatgtt atgactactg aaatgaatgc cataagatgt
     4201 ttaatagatg aagcaggaat agaatatgaa agtatagtta gtgaaattgc aaattgtgaa
     4261 ggcaaagtaa tttttatggg tgtaggaaaa tctgcacata taggtaagaa attggcagct
     4321 acatttgcta gtactggtac acctagcttt tttgtacatg caacagaggc agtgcatgga
     4381 gatttaggaa tgattgaaag taaggatatt acaattttaa tatcaaatag tggaaattct
     4441 atggaagtgg taaactgtat aaaatatatt aaagcaatag gttctaagac aattgcattt
     4501 acttcaaaca gaaattcagt actggcaaaa gagtgtgatt atgctttaat atatcctgca
     4561 aaggatgaag cagatcattt aaatctagca ccaacaacat catctacaat tactttggtt
     4621 ttaggagatt ctattgcatg tgcattatct aagagtagta attttggttc ttcagatttt
     4681 tataagtacc atccaggagg aagtttaggt gagaagttaa aaacagctaa caatggataa
     4741 aagcaaaaag gagagtagtt tttatgttca aaatgtttaa aaaaaaatca ataaaatcac
     4801 ccatatcagg aaaagtaata gagttatcta aaatacaaga tggtgtattt tctcaaaaaa
     4861 ttatgggaga gggtgtagct atagattcta caggagacat tgtatatgct ccaatcaatg
     4921 gaaagatagt agtagtagca gagactaaac atgcatttgt aatagaatta gaaaatggga
     4981 tggaacttct tattcatgtt ggtttagata ctgtaaattt aaaaggcgaa ggttttgagg
     5041 tattggtgag tatgggagaa caagtgaagg aaggaacacc aatgttgaaa attaaccgtc
     5101 ctttgattga atcaaagggt atatcactta ttactccagt tacaataact aatcattcag
     5161 agtacaatat gaatacatgt aatgtaggca atagtgttga aggtgggaaa gatactgtaa
     5221 ttgagtttaa ataaagaact aaaatataag gttaatattt ataaatacct ataaacttat
     5281 gttttataag attgattgta tagatttgtt gtataaaaaa gaactcttta attataaatt
     5341 aaggagtttt ttttattttg taaatattgt ttagatttat ata
//
LOCUS       DACXMZ010000236         5366 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.237, whole genome shotgun sequence.
ACCESSION   DACXMZ010000236 DACXMZ010000000
VERSION     DACXMZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.237"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            68..970
                     /locus_tag="JD016_16895"
     CDS             68..970
                     /locus_tag="JD016_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HAU5001296.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKDRGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
     gene            1582..2595
                     /locus_tag="JD016_16900"
     CDS             1582..2595
                     /locus_tag="JD016_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HAU5001297.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKIFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            2947..3516
                     /locus_tag="JD016_16905"
     CDS             2947..3516
                     /locus_tag="JD016_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HAU5001298.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(3852..4913)
                     /locus_tag="JD016_16910"
     CDS             complement(3852..4913)
                     /locus_tag="JD016_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAU5001299.1"
                     /translation="MILYPIIIGLNFLFINNPIYEELIHSKEYILILSIVQIISALIK
                     LLFWFLIHIFLKNRLVNIKQYLSNKIWIFIDMVCISSFLSILIAIILPPTNLIPMGSN
                     KNIILGNLPYGTYLIILSAILANIGVILLLQPLIENVKMKIEKQANHLKEKYYNLLEN
                     QQIQIRKMRHDMNNHFQMIESYLEIEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNS
                     ILNLRHTSLEENGVDVHFNIDIDNHLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRK
                     VVLKARCEKEYFSYLLTNNKVNPINRKQELFISDKKDSNSHGYGIENIREIVNKYNGK
                     LDISYTESEFSLFLYIRLF"
BASE COUNT         1976 a          535 c          884 g         1971 t
ORIGIN      
        1 ttagtatgca aaattctatg aagcaggaac cctgtgactt tagtcatggg aggttcagat
       61 tggaaatgtg ttgccacaag attattatgg attaatagtg attttaggtg ttattcttgg
      121 agttatgggg gcattttaca actggtttac tctatatgtg cagtctttat acaagaagat
      181 tcctaaaatt ggtacctttg gaaaactgtt gattccattt ataatggctg gtattcttgg
      241 gcttgttatg cctgatgttc tggggagcgg acacaattta gttgaagaac ttacaagtca
      301 tgaatatatt tttggaatgg ttgttttgat ttttgttata cgttttatat tttcggctgt
      361 tagttttggg tcaggtgcac cagggggaat attttttcca ttgcttgttt taggagcatt
      421 tataggtgga atatttggta tggtaggtgt taaagttttt ggaatgaatc ccgattatgt
      481 aaataacttt gtgcttttgg ctatggcagg atattttaca gctattgtgc gtgccccttt
      541 gactggtatc atattaattt ttgaaatgac aggttctgtt agtcaaatgc tttcactatc
      601 agtaatttca attgtggcat atattgtggc tacacttatg aagtctaaac ctatttatga
      661 aagtcttctt gagaggttac ttgaaaaaaa tggagaacct gtacataaag ataggggcga
      721 aaagatacta gaccagttct ctgttatgtt tggctctcct gttgcgaaca aggcaattca
      781 ggatgtacag tggcctgaaa attgtctttt ggtagcaatt caaagaggtg gagaagaaat
      841 tattcctaga ggtaagacaa ttcttagacc aagtgatgta attgtgacta tgacagatga
      901 gagagatagt ggtgctgtgt atgataagat ggaaaatttg tgtaaagaac atacggcaca
      961 tattttttag ggagaatgat gaatgtttaa taatgataga atatttaatg aagatttgat
     1021 taaaaaattg gtaatttgaa agagagttaa ggaattaata taagaaaatg aaaatgtgat
     1081 atcttagatg taaaagagtt tgaagaaaat aattgtatat atttgttaat gagattatat
     1141 ggactaatgg tatatttata ctgtgctaaa agttgtacta ttgtatgagg aattggaata
     1201 aagggtttga aaaaataggt agaagatttt tcagatattc ttattgggat attttaatta
     1261 atatatttta attgcgatat tttgggaaga atagaattta tttataaatg aaaataatta
     1321 aaaggggtta aggttgtatt agcccctttt gcttatagag tttggaaatg gtatcttgat
     1381 agtagttgtt tcatgcttat tatattttat tacatatttt aaaattgacc ttattactcc
     1441 taaatcattg ttatatcaac ttttaaaaca ctttcaagac aatcgcctat tgaaaacaaa
     1501 gttaattaaa aacctacata tcagtttagt attcgtgtag atgattatat aaatataaaa
     1561 gtataagaaa ggtgagtatc tgtggttagt tcatttttaa atgatatctt tagaacaatt
     1621 ttagtaagtt gtatttttat agttatttta ttagtcttta gaataacttt atttaagatt
     1681 ttcagtaaaa aatttaatta ctatatatgg cttattgtga ttattaagct atcattacca
     1741 tttatgaatt atacttttat atttaatgag tcaaaatata atggtaatat taataagata
     1801 agtttaggca attttaatag tatttctatt gtatatagta ctgttttagt ctctgtttgg
     1861 attgctatta cgatatttta tttagtttat actttattga aatatattaa gcttaaaaat
     1921 atgataaatg atttgtctta tgatgttgag gatgataaga taaaaaatct ttatgaaaat
     1981 ttattaaaag agcttaagat aaataaaaaa atacggctta aatattcata tgaagttgaa
     2041 agtccagtat tttttaattc gtgtgtcatt ttaccaccat gtgattatac attaaaagag
     2101 ttaaactgga tatttaggca tgagttgatg cattttaaga gtaaagacct ttatattaag
     2161 tatttggtga tatttttaaa atgtgtttat tggtttaatc cttttgtgta tataatggag
     2221 aagtttatag atttggattg tgaattttat tgtgatgaaa gagttttgaa gaattgtacg
     2281 atagaagaga agcaggatta tgcattgaca ataattagtg caatgagaaa aggttcaaat
     2341 agttcaaata aatttgtggc tggattacat aaggagtcag atattaaaaa aagagtgttt
     2401 agtatgttta atgaaaagta cagaaatggt attttgatag cagttatttt atgtttatta
     2461 tcttctatta catattttaa aattgattta attactacta aatcaatatt ttatccactt
     2521 tcaaaacaaa cacctattga aaacagaact aattcaaaac ctacatatca gattaatgtt
     2581 tatatagata attaaaaaat acaaagttta acaagagatt aatataaata attttaataa
     2641 gtatctttca catatgttaa atttacttta attatattaa agccaatgaa ttattcttta
     2701 gaagctccaa tgccagtaaa tacaggtaaa gaaaaaatta ctacaattgt agattaaaaa
     2761 tgttctaata tttaaagaaa tgcaaaagtg taagaagggt aaaaatctgt tataatacat
     2821 taaaaactaa atttagaaaa atttacataa ttagaaaaat gatttgataa aaaataaagt
     2881 taaatattaa ctaagtaaat ttgaaaaaga aaaaatataa agtacaaatt taaaaggagg
     2941 taactaatga aaaagaagtt ggaagatatg actttagagg agttatggaa cttgtttcct
     3001 atatttttga ctactcacaa agaatgttgg aaaaattggt ttaaagaaga acaatataga
     3061 ctaactggaa tattgccaat gcaaaaaatt gttagaataa gtcatattgg aagtacttct
     3121 gtgagtacga tttatgccaa gcccattgtt gacatactga ttgagataat tcctgattat
     3181 aatatgaaag atattaaaga tttgattgag tcgaatggat atatttgtat gtctgagagc
     3241 agtagtaggc tttcacttaa taaagggtat acagtagatg ggtttgaaga acgagttttt
     3301 catttgcata taaggtatgt aggtgataat gatgagttgt tttttagaga ttacttaatt
     3361 gaccacccag atgttgcaaa ggagtatgaa aaacttaagt tggacctttg gaagaagtat
     3421 gagcataatc gtgatgctta tacagatagt aaactagatt ttgttaggga gtttactgaa
     3481 aaggcaaagg tgttgtattc taatagatat aattagaaat aggtacaatt agatatttat
     3541 cttatatatt gtattatatt ttttaagaaa gtaattaata gaaaaagggc ttgtttcaaa
     3601 atggagagat aaatcccttt ttcattgttg aaaggataaa aatatatttg atttcatgta
     3661 aaaaagagtg cctcatcaac taaaaagttt attttgagac acacacattt ttactaaggt
     3721 aaataaaata tcacatttga ttaaatttta agttaatgaa tatatgtgta aatatttaat
     3781 tataagttat tataatttta catagtatca aacaatctaa tttgtattat ataagataat
     3841 ctaattttag actaaaataa tctaatataa agaaataaag aaaattcact ctcagtataa
     3901 gagatatcca attttccatt gtacttatta acaatttctc taatattctc aataccatat
     3961 ccgtgagaat tactatcttt tttatctgaa ataaatagtt cttgttttct attgatagga
     4021 tttactttgt tgtttgtcaa taaatagcta aaatactcct tctcacaacg tgcctttaat
     4081 accacttttc tatttgacaa atcaggaatt ttcaaagatg cttctatagc gttatctaaa
     4141 gaatttgaaa aaatagtaca taaatctata ggttctatac ccaagtgatt gtctatatct
     4201 atattaaagt gcacatcaac accattttct tccaaacttg tatgacgaag atttaaaatt
     4261 gagttaagag cttggttttt gcaaaattgt ttgccactaa gagtacctat acctattttt
     4321 aactgattaa aatacttttt agctccagca atatcttcaa tttctaaata cgattcaatc
     4381 atttgaaagt gattattcat atcatgtcgc attttgcgta tttgaatttg ttgattttct
     4441 agtaagttat agtatttttc ttttaggtga tttgcttgtt tttctatctt catctttaca
     4501 ttttcaatca atggctgtaa aagaagtatc acaccgatat ttgccagaat tgctgacaag
     4561 atgattagat aagtgccata aggtaagttg cctaagataa tattcttatt actacccata
     4621 gggattaaat tagtaggtgg cagaataata gcaatcaata ttgataaaaa tgaagatata
     4681 cacaccatat caataaaaat ccatatttta tttgataagt attgttttat attgaccaat
     4741 ctattcttta ggaaaatatg tattaaaaac caaaataata atttaattag agcagaaatt
     4801 atctggacta ttgaaagtat taaaatatac tcttttgagt gtataagttc ctcatatata
     4861 gggttgttta taaaaagaaa attaagccca attataatag gataaagtat catgacaact
     4921 gatattatag gaattaattt gcttttgcta catattatat aagtagtata aaatatccga
     4981 atagtgtaaa caaaatattt acggggtcgg aaagccatat tatcatttga ctaataatag
     5041 agtagcccaa aatcaatatg attttgaaaa tccaatgatt cctaaagtct ataaaatagg
     5101 aagtcaactt ataaagaaaa aaggctatga aaatattgat taatattaga ataaaattta
     5161 tgcttgtaga gattaaagaa taagtgccca tttaatatac ctttctatta attaagattc
     5221 tggaaactaa gttttaaaaa attaaatctt aaaaattaaa attctggaaa ttagatttca
     5281 aagaattgat tttgaaaaat aaaattctag agattgaact tcaaaaaatt gagttttaaa
     5341 agattgaaat tttaaaaatt aagttt
//
LOCUS       DACXMZ010000237         5349 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.238, whole genome shotgun sequence.
ACCESSION   DACXMZ010000237 DACXMZ010000000
VERSION     DACXMZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.238"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(204..1388)
                     /locus_tag="JD016_16915"
     CDS             complement(204..1388)
                     /locus_tag="JD016_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAU5001300.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLIKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="JD016_16920"
     CDS             1761..4112
                     /locus_tag="JD016_16920"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HAU5001301.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKKKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="JD016_16925"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="JD016_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAU5001302.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2093 a          598 c          851 g         1807 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttacct tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaattta
      241 tctaatccag ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttatta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtatgggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caaggttgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagagcat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaaaagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaacac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actattccct aattttagct
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgcc ttgaggtaca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta ggaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gcttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tatcgataca ttatttaata
     4861 gattcttatg ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat
     4921 ctagatatgt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatatta agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac
     5041 taaacacata agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat
     5101 gtaatattta tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat
     5161 aatgtggata atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg
     5221 gataatatta taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt
     5281 tgttgactct aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca
     5341 aataaagca
//
LOCUS       DACXMZ010000238         5344 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.239, whole genome shotgun sequence.
ACCESSION   DACXMZ010000238 DACXMZ010000000
VERSION     DACXMZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.239"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(214..930)
                     /gene="dapD"
                     /locus_tag="JD016_16930"
     CDS             complement(214..930)
                     /gene="dapD"
                     /locus_tag="JD016_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAU5001303.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     NNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(1154..1903)
                     /locus_tag="JD016_16935"
     CDS             complement(1154..1903)
                     /locus_tag="JD016_16935"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAU5001304.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(1970..2857)
                     /locus_tag="JD016_16940"
     CDS             complement(1970..2857)
                     /locus_tag="JD016_16940"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAU5001305.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(2974..3975)
                     /locus_tag="JD016_16945"
     CDS             complement(2974..3975)
                     /locus_tag="JD016_16945"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAU5001306.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(4400..5281)
                     /locus_tag="JD016_16950"
     CDS             complement(4400..5281)
                     /locus_tag="JD016_16950"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAU5001307.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDALL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPFVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
BASE COUNT         1759 a          969 c          652 g         1964 t
ORIGIN      
        1 ttgaaggaaa taaaaatact tgtcagcaag aaataaaaat gatataagaa tgaataagag
       61 aattgtaaaa aaataaaatg attataaata aaagataaaa atattgtaaa aaagagggtg
      121 cctctggtga attaaattca cacagagaca cctttttaat taatttttta aattagtata
      181 ttattgttta attaatagca tagtactatt taattaatct aaatttctaa ggtcttccat
      241 aagcttagtt ttgtcagccg ttttttcatc tttcattttt ataactttag caggagaacc
      301 agcaacaacg gcaccagctt ctacatcagt agttacaaca gcaccagctg caacaacagc
      361 acctttaccg attctaacac cttcaagaat aactgcatta gctcctatta aaacatcatc
      421 ttcaactata acaggagtag cagaaggtgg ctctaaaaca ccagcaacaa cagcaccagc
      481 acctaagtga acatttttac caagagttcc tctagctcca ataacagcat tcatatctac
      541 catagaacct tcaccaataa cagctcctat atttataaca gctcccatca ttacaacagc
      601 atttttttct atagtaacca tgtctcttat tattgcacct ggctcaattc ttgcatgttc
      661 acttaaagta ttttttagag gaatagcaga atttcttcta tcatattcta catgagtatc
      721 tgttatatca tttccatgtt tatctaatac ttccattata tcctcatagt cacctacaaa
      781 tatataagaa ccattatttc caaatacctt aaatttatca gttgattgga agtgtaaaga
      841 gttagtattt acatatacct taacaggtgt tttcttttta acttctttta tataccttgc
      901 tatttcataa gcatcgttta aatttaccat ttgttaattc tccttgtatt tcattatatt
      961 tcttttatta tatacttatt tgtcaatttt agtatatact tacttcttat tatttatatt
     1021 ttattttagt tagtttttat tgtatagaca tagtattaaa taaatttatt atatatgttt
     1081 gatttgtatt gcatattatt atatatatat tgtatatgtt taatttatat tgtgtattat
     1141 tatatttata ctattatccc aacatgctgt ccatattata aaatcctggt ttttgcttaa
     1201 ctaagtattt ggcagcagct atagaaccac ttgcaaagat accacgagat tgagcttgat
     1261 ggcttattgt aagaacctca ttgtctccag caaatagagc atcatgttcg ccaacaatat
     1321 ttccacctct tatagcatga ataccaactt catttggttg tctttttgca ctacgaccat
     1381 atctaccata attattttca acgtttggca aaacctcttt tatagcgtta gcaatcataa
     1441 cagctgtgcc acttggggca tctacttttc tgttgtgatg tttttctatt atttctatat
     1501 caaatccttc tagtaattta gctgcatctt taaccaattt tactaataca tttacaccaa
     1561 gagacatatt atatgattgg aatacaggaa taactttagc cgcttcacgt attttgttca
     1621 tttcgtcatc attatatcca gtagtagcaa ttactaaagg tgttttagtc tttaaaacgt
     1681 aagataagat attatctaaa ttagagtgat gagaaaaatc tataactaca tcagcttcct
     1741 caactatagt actcatatct tcataaattt taaggtcgcc ttcatagcta tccagacctc
     1801 tagctgcaac acaaaccaat tctaattcat tatcttctct tatagtatcg gctaaaatct
     1861 tacccatttt tccatcataa ccagttgcta taacttttaa cataatttcc tcctatgatt
     1921 aagggctaac ttttgaccaa acttactttt taccatgcat taatctttac tatgcattaa
     1981 cttttagccc aaagttaact aattctttct ttaatacttc taaatttgct gggtccattt
     2041 ctgctagagg taatcttaaa tctccaacat tgaatcccaa taaattcatt gcagtcttaa
     2101 ctggtatagg atttacttct ataaataaag ctgcgattag agcatccata ccaagttgta
     2161 attttctaga accttcaata tcgccttcaa agaatttagc taccaaatcg tgagtttctt
     2221 gagggcaaac attagcaagt actgatataa ctccttgacc tcctaaagat aataaaggaa
     2281 gtatagtatc atcattacca gagtagatag caaagtcatc tggaactaaa ctagctattt
     2341 ctgcaacctg tcctaggtct ccactagctt cttttacagc aactatgtta tcaattttag
     2401 caagttcaac tataaggcta ggacttatat ttacacaagt tcttccaggg acattgtaaa
     2461 gtataattgg aagtttaaca ctgttagcta ttgtttcaaa atgacgctta agtccagctt
     2521 tattagcttt attgtaataa ggtgttatta ttaatagagc atctactcct aatttttcag
     2581 cttcttgact taagttaact gagtgcatag tatcattaga accacttcca gctataacag
     2641 gaactctttt attaactttt tcaacaacat acttaatagt agcaagttgt tcttcatcag
     2701 tcatagtatt agcttcacca gtagtaccac aagatactat agcatcagta ccattagcta
     2761 tttggtattc aactaattca cctaatttgt caaaatcaac tttgttatcc tttgtaaatg
     2821 gtgtaactag agcaacagca gaacctttaa atatcatata aaatcaatcc cctctttttt
     2881 atttttgtgt tttatattac agttacttct aatgcttgtt tttgagttac tattaatatt
     2941 ttacaaaatt atatatttat acaagtttgt atattatact aaatcgtatg ataaagccat
     3001 ttcagctatt tgaattgcgt tagttgcagc accttttctt atattatcag caacaaccca
     3061 aagatttaca ccattatcaa cactaaaatc tcttcttatt cttccaacaa atacttcatc
     3121 tctatcatca aattctacag cagttggata ttcgttttta gcaggattat caactactac
     3181 aacacctgga gcttcttcaa gtgtttttac taaatcatct aactcaaatg gattgttaaa
     3241 ctcaacattt atagattcac tatgaccatt ttttactgga actcttacag tagttgcagt
     3301 tatttttaaa tcgtagttat caagtatttt catagtttca tttaccattt tcatttcttc
     3361 tttagtgtat ccattttcag tgaatgaatc tatatgagga agacagttgt aagagatttg
     3421 atgagggtag aatccagtag gattaccgtt cattcctttc tctaaatctt caacaccttt
     3481 aacaccagaa ccagatacag cttggtaagt agagtaaaca actctcttaa ttttgtattt
     3541 atcatcaaga gctttaaggg gaaccatagc ttgtatagta gagcagttag gattagctat
     3601 tataccctta tgatttttta tggcttctgg attaacctca ggaactacta aaggcacatc
     3661 tggattcatt ctccattgac tagagttatc tactactaag attccatttg aagcagctat
     3721 tggagcatac ttttcactaa tacttccacc agcagagaat aatgctattt gtatgcctct
     3781 atcagtaaat gaagtttcct ttaattcttc tactatatat tctttaccac aaaacattac
     3841 tttagaacca gctgatttag aagaagagaa taaaaataga ttttctattg gaaaatttct
     3901 ttcttctaat acttttaaaa atgttctacc taccattcca gtagcaccaa ctattgctac
     3961 atttactttt ttcatataaa ttcaccccaa ttttaaatta ttaaaacatt attttatttt
     4021 agttatacaa gtaagatatg ttaatatttt tatatatcca aaaactttaa tattatatta
     4081 acaactttaa ttatatacaa aaaacgtaca aaggtatact ttgtacgtct aatttatcac
     4141 tcaaagtaaa ccctgtagct catcacctac aaaaataagt gacagtatta cacatgttat
     4201 gtgcaatccc agaaaatatt attgcaagaa atattttctt cggctgtaaa tcctttttcc
     4261 aatcattaac acctgccagc tactattgga tactcttagt tacagctacc tctaccctag
     4321 gttaatagga tattaaaatt tgattattct aataataata catatttaag aaaaaatcaa
     4381 cttatttata taaatattgc taaaatttaa atccaaaatt tgtcaattct tgttttaaca
     4441 cattaagatt agtttcttcc atttcagcta gaggaagtct taaatcacca acattaaaac
     4501 ctaaaatgtt catggcagtt ttaacaggaa ctggatttac ttctataaat aaggcagcaa
     4561 ttaaagcatc catatcaagt tgtaattttt ttgagccttc aatatcacct tcaaagaatt
     4621 tagttactaa atcatgtgtt tctttaggac atatgttagc aagtacagag ataacaccac
     4681 ttccacctaa tgataatagt ggtagtatag tatcatcatt accggagtat atagcaaagt
     4741 ctttaggtac taatttagct atttctgcaa cttgagctaa gtctccactt gcttccttca
     4801 cagcaactat attatctatt ttagcaagtt cggccacaac aaatggcttt atattcactt
     4861 tagttcttcc tggtacgttg tataaaataa ttggcaactt aacgctattt gctattgttt
     4921 caaagtgaag tttaagacct ttctcattag tcttattata gtaaggagtt attattaaaa
     4981 gagcatccac ccctaatttt tcagcttctt gactcatatg aacagacaac atagtattgt
     5041 ttgaaccagt acctgctata acaggtattc tcttattaac tttttcaact gtatatttta
     5101 ttacagcaaa aatttcagat tcactcatag tagtcgcctc acctgttgtt ccacaaacaa
     5161 ctagtgcatc tgtgccattc tcaatgtgat attcgattaa ttcacctaat ttttcaaagt
     5221 tgacattatt atcctctgta aatggggtaa ctaaagcaac agcagaacct ttaaataaca
     5281 tataatcaaa tctccttttt tatctaaatt taatttttta agtacaataa taattatagt
     5341 acaa
//
LOCUS       DACXMZ010000239         5293 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.240, whole genome shotgun sequence.
ACCESSION   DACXMZ010000239 DACXMZ010000000
VERSION     DACXMZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.240"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            136..279
                     /locus_tag="JD016_16955"
     CDS             136..279
                     /locus_tag="JD016_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001308.1"
                     /translation="MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNK
                     KRH"
     regulatory      complement(482..579)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            790..1179
                     /locus_tag="JD016_16960"
     CDS             790..1179
                     /locus_tag="JD016_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAU5001309.1"
                     /translation="MEQQEVVNKLRRGELMLMEYLWRKKSILSKKEIIEAMKDRYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFQFSYEVLVTKKEYLNARKEDRQVTKYDSFFIRMF
                     TTMHKKEKMTEEQIKKYIENMEKIGKE"
     gene            1271..1654
                     /locus_tag="JD016_16965"
     CDS             1271..1654
                     /locus_tag="JD016_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAU5001310.1"
                     /translation="MGFKKLPDSELKVMKFIWGLDKNKVASGEVVEAMKQIYDWNKNT
                     TLTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTAL
                     NDEEDLSDDKLDMLEQWVKNWEEDE"
     gene            1803..1927
                     /locus_tag="JD016_16970"
                     /pseudo
     CDS             1803..1927
                     /locus_tag="JD016_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2372..3763
                     /locus_tag="JD016_16975"
     CDS             2372..3763
                     /locus_tag="JD016_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAU5001311.1"
                     /translation="MRNKIIIGITFSLVLLMSGCASKQTAEINVLATTDLHGVIPYEL
                     TSYVKEERKKDKNLTLVDAGDFFDSGEVSGSSMDSYFSARYKDIDNNTERYIETPISK
                     DMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTYKKNGDSYTKPYII
                     KNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQEGYNGELYMNDLVE
                     DAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVDGIDAIVAGHNHVQ
                     IEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDKSSKLIKFEKNQES
                     DKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDIYKTIGYKWRNISS
                     PKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVTIYPNKNDKFKIEK
                     GKQEYSINLKHIQ"
     gene            complement(3911..4261)
                     /locus_tag="JD016_16980"
                     /pseudo
     CDS             complement(3911..4261)
                     /locus_tag="JD016_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            4256..4777
                     /locus_tag="JD016_16985"
     CDS             4256..4777
                     /locus_tag="JD016_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HAU5001312.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKIFTDIITISQMTPGPL
                     AVNTSTFVGLQIVGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(4906..5142)
                     /locus_tag="JD016_16990"
     CDS             complement(4906..5142)
                     /locus_tag="JD016_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001313.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     YKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
BASE COUNT         2090 a          567 c          843 g         1793 t
ORIGIN      
        1 atgaagaata gatatttgat aattgtaaaa ttatgttata attaaaatgc aagaatatat
       61 cttgtaagaa actacaatcc aagagtggag cttcattttc tacattccat cccttaaaag
      121 gaaggaggtg gagaaatgag cgaattttta ctaggagtgt tagctagttt aacagctagc
      181 tttattacat atattatttc cagaaaagta aaaagccact ctggcaggag tgactttgag
      241 cttgatgtaa aaatcaagtt taataaaaaa cgacattaat atttagttaa tgaaacttca
      301 ctctaggcta atagattgta gtttcttttt ttgtttttga tacgaattta catctttatt
      361 atatcgcatt ttaagaaaaa ataaaactgt gaatgctaga aatatatttt attttatctc
      421 taagtatata tattattatc cccctaaaaa actttcataa aaacaaaaag aacaatatct
      481 ctttggcaac tggctgacat aactcataag atatttatat aagtcttagt ccctatagct
      541 ttgcgtcact aaatttctct agttttgccg atttagtttt actctacaac tacaataata
      601 cattagaatg agtcattatg caacatgctt gtttgaaaat taaataaatt tataggtatg
      661 aataatattt tttgtatgta aaaacaaact aaaaacgtag aatatatggt aaaacttacc
      721 tgtagttttt gtcctaaaaa gctcctatta ttaacttaaa ttaagtttaa tttagggaag
      781 tgacataaaa tggaacaaca agaagtagtt aataaactgc gcagaggaga actaatgtta
      841 atggaatact tgtggaggaa aaagtctatt ttatctaaaa aagaaataat agaagcaatg
      901 aaagataggt ataagtggag gaaaagcact acagaaattc ttttaaaaag attagtaaag
      961 atgaaaacat taaaaaagaa gaggataggt tttcaattta gttatgaagt attagtaact
     1021 aaaaaagaat atttaaatgc tagaaaagaa gatagacagg taactaaata tgatagtttt
     1081 tttatacgaa tgtttactac tatgcataaa aaagaaaaga tgacagaaga acagataaaa
     1141 aaatatatag aaaatatgga aaaaattggt aaagaataat ttatatatat attaattatt
     1201 tttaaaataa atttgttata atatttaaat atacataaac atacataaat ttacaaaggg
     1261 ggtgtggctt atgggattca agaaattgcc agattctgag ttaaaagtaa tgaagtttat
     1321 ttggggatta gataaaaata aggtagcatc aggagaagtt gtggaagcaa tgaaacaaat
     1381 ttatgactgg aataaaaata caacattaac aatattgtct aagcttgcaa agaaaaacta
     1441 tttgtattct caaaaaacaa gcaagtgtac atattatact atcgctgtaa gggaaaaaga
     1501 ttatttaaaa gttgaaacaa aaaaattttt tagttttttt cataataatt ctttaaaaag
     1561 ttttttcact gctttaaatg acgaagaaga cttaagtgat gataaattag atatgttaga
     1621 gcaatgggta aaaaattggg aagaggatga ataaaatgaa tgtgatacta aaatgatact
     1681 aatatgtttt atagcgtgtt aaaaaatata gaaataatag aaataataga agaaaattca
     1741 tgtaatagat gaggttttat cagattaaaa agaaaaacaa tagaatggga ataaaatata
     1801 gaatgccaaa aatccacatg aatacaggat ttgaggtctc tcttacttct atgttatact
     1861 ggtttgatac tatcaatatt tgccagtatc ttatggatta ataagcaaaa aacatcctgc
     1921 tgaatgataa attcagcggg gtgtttttat tttgttttag aactcctttg ctctgaaact
     1981 cgaagtattt aacactgatg atgttgttat cttttagttc cttttgtgtc atgctctgaa
     2041 atacaaattg atacaataaa aaagtaattt cttaatcatt gatggagata tgtatttatg
     2101 ttactcttga tggtaatttg agtaatattg ttcatagctt tttatattca cttaattaga
     2161 aaaccatttg gtataatcat aaataaactt gatgatataa tagaaaagat ttataattat
     2221 tagaagtata taaaagtatt ttatgtctat gaaatgtatg tctataaaac atatagatga
     2281 cttttgtata ttaatgtatg aatattgata ttatagaaga attgagttgt caatgatact
     2341 aaatcaaata tttatagaag ttggagggat tatgagaaat aaaataatta taggaattac
     2401 tttttcactt gtattactaa tgtctggatg tgcttcaaaa caaacagcag aaataaacgt
     2461 attagcaact actgatttac atggagttat accttatgag ttaacttctt atgtaaaaga
     2521 agaaagaaaa aaagataaaa atctaacttt agtagatgca ggagattttt ttgatagtgg
     2581 cgaagtatct ggttctagca tggatagtta ttttagtgct aggtataagg atattgataa
     2641 taacactgag cgttacattg agacacctat ttcaaaagat atgaaagagg taggttatga
     2701 tgttgcagtt cttgggaatc atgaatttat atccaacaat aaattttatc tagataatat
     2761 gatatctgat tttgagaaac aaaaaataaa tattttatcg gcaaatacgt ataaaaaaaa
     2821 tggagatagt tatacaaaac catacataat aaaaaatatt aatacacctg aaggaaacgt
     2881 aaaactagga attttagggc ttactataaa agaagttggt gagaggaaac agtggaaaga
     2941 tggaaaactt gttgactcta agtctttaga actaaaagac caagagggtt acaatggtga
     3001 attgtatatg aatgatttag tagaagatgc taaaaagtgg gttggtataa tggaaaaaga
     3061 gaagcctgac attatagttg ctgtggcaca tacaggtgag aaacctaaga aacctaaaaa
     3121 cccaggaaat agaatacaag acttagctca acaagttgat ggaattgatg ctatagttgc
     3181 aggtcataat catgttcaaa ttgagcaaca tgattataaa aataaatcag gagaaaatgt
     3241 tatagttact gagccaggaa agcatgggga atgtatttct aagattaatt ttaaattaga
     3301 aaaaaacaat gataaatgga atatagttga caaatctagt aaattaatta aatttgaaaa
     3361 aaatcaagaa agtgataaat ttggagaatt tatttcttca attaataaag taagtaaaga
     3421 taagaaagaa ataagattat cagaagtaaa gactcctgaa tgggataaaa tatatgtttt
     3481 taaatctgga acatctgcag atgatatata taaaacaata ggatataaat ggagaaatat
     3541 atcatctcca aagggtgatg acatggtgca gattgttgta atgaataata ataagcctgt
     3601 tagctatgta tatggtgaag ctgaagttat gccaattaat ataaaatgtg atgaatcaga
     3661 gtataaagat aatgttgtaa ctatttatcc taataaaaat gataaattta aaattgaaaa
     3721 aggtaaacaa gaatatagta ttaatttaaa acatatacaa tagaaacttt tatcttattt
     3781 aagtttagat tgtacttaaa ataaagtgtg tattatttta aaaattgatg ataattaaaa
     3841 tcacaacatt aaggaatagt acacactttt tatgctaaaa aatagaatgt taaaaaatta
     3901 aacctttaaa ttaaagtaca ttgcttatat tatccaatat tatcttcaaa ggttgtgtaa
     3961 tatttttatc cttatgtaat attgctaaca ttttattttc catatctatc ccatctaatt
     4021 caatcaatcg caatttttta tgaaagagtg aatctaataa taaattttct ggcaaaatgg
     4081 ttatacctaa ccctgcttct gcagctttaa taagtgcaaa agaattaaca ctttcccaaa
     4141 caggataagc tttttggttt gaaagagata atacactgtc caaagtattg cgaatggcac
     4201 ttccttgctc acgtaacaat aacggaaacg tgcataactg tcgtagagat aaggcttgtt
     4261 tagtattggt ggtggttatg caatcatacc tttaatccaa gagaaagtag taaatcaaac
     4321 tggatggata agtgagaaaa tatttacaga tattataact atttcacaaa tgacaccagg
     4381 gccactggct gttaatactt ctacttttgt tggacttcaa attgtagggt tttggggagc
     4441 agttgcagcc actttaggtt gtgtattatg tggtgtagta atatctctat ttttatataa
     4501 tttttttcaa aagcatcaga actctactta tatatttgaa atattaaatg gtctaaaatc
     4561 agcttcatta ggtttaatta tctcagctgc ggctacaatt ataattcttg cattatttgg
     4621 gacgaataaa gtgtacttca attctatatt tgagatgcta aactggactg cactaataat
     4681 cttttttgtt acactactga tagtacgtaa gtggaaaatt aatcctattg tgattataat
     4741 aataagtggt atactgggaa tattttttta tttataagtt tttgctaagt aaaggatatc
     4801 tattttttta tatcttaaat ccaaatagcc aataaagtaa ggctatttgg atttttaata
     4861 aattctgaaa agataatcag aaacccatat aacatttaaa ttatatcact ggttttctac
     4921 aaaccaatca taagcttctt ggcaaaattt ctcactatct atatagtaac tattatgaac
     4981 attattaatt tcaacaagtt tctttttata ttttagtgaa ttataatagc tatcaaaata
     5041 ttgggtttta aaatatctat catgaagtgt tttaaagaca agagtaggta tgttgactcc
     5101 atcttttatc ttactatctt ctgcaataaa gcattctaac attgtattaa ttatctttgt
     5161 actatttttc caaatatgag gatttttctt ttcaaatatt ttataaaatt cattatcata
     5221 gacttcatta taacctttat atttaggtgg ttttactgtt atgtgaaaat taggaagaaa
     5281 cttaaagaaa aaa
//
LOCUS       DACXMZ010000240         5217 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.241, whole genome shotgun sequence.
ACCESSION   DACXMZ010000240 DACXMZ010000000
VERSION     DACXMZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.241"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            491..1798
                     /locus_tag="JD016_16995"
     CDS             491..1798
                     /locus_tag="JD016_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001314.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1884..2146
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2272..3075
                     /locus_tag="JD016_17000"
     CDS             2272..3075
                     /locus_tag="JD016_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAU5001315.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3113..3724
                     /locus_tag="JD016_17005"
     CDS             3113..3724
                     /locus_tag="JD016_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAU5001316.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3756..4427
                     /locus_tag="JD016_17010"
     CDS             3756..4427
                     /locus_tag="JD016_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAU5001317.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4726..5088
                     /locus_tag="JD016_17015"
     CDS             4726..5088
                     /locus_tag="JD016_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAU5001318.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         2107 a          464 c          921 g         1725 t
ORIGIN      
        1 gggaatattt acagtttaat ggcagtttta aaataataaa ttaaagctgt catttttatt
       61 taaaaatttt tagaaaaagt acaatagtgt taatgtgtga attagataag gtgtatataa
      121 tagtttagat gatatgatat aaaatatgtt gtaaattaaa tgttttcttt gacaatatat
      181 acacaaggat gataatatct atatgaataa aagatagtgt gtaatttgat aatatgtaat
      241 tataacatta tgatataatt acaataaagt atgtaataat ctgtagaaat ttcaagctag
      301 ttatctaagt tttcggataa ctaaatctaa acggaataca taatgtatta aataaatgat
      361 gatactaaaa tgatactatt tttatagaaa tttattgagg tatataatta atgaaaataa
      421 tatattaaat tatataaatt aaattatata atatattgag ttttacataa aagcaaaggt
      481 gggaataaaa atgaaagaaa ttgctgatgc tataacatca gagtatttaa gctatggtat
      541 caatgaagat attagtatct atgaaggtag attttgcata tatttagata aaaaatatag
      601 atgtaatggg aaaatttatt ataaaatgac acctccaata tctataagtt ttaaagcaga
      661 cataggttgt gttgaagaga tagacaatga agatgacaat ttagctttag attatgataa
      721 tgctattctt gaagttcatg gatataaaat tatttctata acaataaata ctttaagtga
      781 atttagtgta gaaggttata tcaatgatga ctgcataaaa tctaagaact cctatgttga
      841 atatgtagat tttaatataa taaacttaga taaaatacct ggaaaactca ttaaatacaa
      901 tgataaggta tacgcaggta gaattgaatt tgatataaat gattatgttg taactataga
      961 caagcgatat gattatagaa aagaattaaa atcagaatta aagtctaaat ctggagcaat
     1021 aataactcat ataggcagga ttagaagaaa agatggtaga atttttagaa caaataatac
     1081 aataaatctt ttagatagga tatcaacagc tttgagtttt atgtgtggaa gatatgtagg
     1141 gttttgtttg gccaaagggt atagaagtgg taatgaagtt tatagaatat ggaatgaaaa
     1201 tcaaatatct ccttttagat atgtaccaac atggtcagat acactttcaa attatcataa
     1261 tatggaaaaa tatataagcc ttatgtgtaa aaagctagaa gatttttatt atggttctgc
     1321 tataaagagt gttgttgatt ggtacataga atctcttgga agtgctacca tggaaaacaa
     1381 tataatatct gtacaaattg cacttgaaac tttgtcatat gtaattctag tggaacaaaa
     1441 taagatactt acagatgaag catttgactg taatttggca tctaaaaata taagactctt
     1501 acttgataca tgtaagatac catatggtaa acatgaatta aatatatttg ataatataat
     1561 aaaaaataaa tttgatgatg gagtggattt agttatttat ttaagaaata gcatagttca
     1621 tccaagtaga aagactcata gagctgtgtt agaggttgaa gatatttgga atataatttc
     1681 aataggaacg agatatattg aacttgtttt actctttatt ttaggttata gaggagaata
     1741 ttcaaacaga ttagtagaaa gatgttatgg tgaagtggaa gttgtacctt ggaactagat
     1801 ttttatattt attttaaaat atatattgac atatattgcc ttatgctata aaatcaatat
     1861 cataataata attcagaata acattaaatg ctaagaaaag aaatagtaaa agtagttaat
     1921 cttaaacaga gagtttccga ttggtgagag gagcaataga ttttactttg aacacatctt
     1981 tgagcaggac tctgaaagag tagaaatact ttattaggag atttccggaa tccttgcacc
     2041 cgttatagtg ctagagtata ataattttat tgtacttaaa gaggttagtg tcgtgaggca
     2101 ttaatgaaca gaggtggaac cacgagagtt aactcccgtc ctctagcaga agctagaggc
     2161 gggagttttt taatataatt ttttaagcgc gcagagaatt atattgcaat agatacactt
     2221 tttaaaaaat aatcaaaata ttttaaaaat taaaagacga gggagaatat tatgaaaaat
     2281 atattaaaaa aagtaggaat atttacaatt atgttaggtt tattaggtgg agttgtaggt
     2341 tgttcaaaac cagataatga aaaagataag gatgcttcaa aggaatctaa aaaagaagta
     2401 gttgtagggt ttgataatac gtttgtacca atgggatttt tagatgaaaa gggagataca
     2461 gtaggctttg acgtagactt agctaaagaa acatttaaaa gacttggaat ggaagttaag
     2521 tttcagccta tagattggtc aatgaaggaa actgaattaa atgattcaaa aactgtagat
     2581 gtattatgga atggatattc tataacagac gaaagaaaga aaatagtatc atatacagaa
     2641 ccatatttac aaaataaaca gataatagtt actctaagtg actcaaaaat aaattcaaaa
     2701 gctgatttaa aagataaaga agttggaaca caacaaggtt caacagcact tgatgcagtg
     2761 gaaaaggata aagattttat gaatagctta aaaggtggag caccagttct ttatgatact
     2821 tatgataaag cacttagaga tttagagata ggtagaacta gtgcagttgt tggtgatgaa
     2881 gttttaatta gatactatat gggacaaaag ggtgaagaca aatacaaggt attaaaagac
     2941 gattttggat tagaagatta tgttgtagca acttctaaag aaaatccaga actttgtgag
     3001 aaaataaatg aaacattaaa agaaatgaaa aaagatggaa cttttgacaa gatttatgat
     3061 aagtggttta aatagaagat aagttagctg ttaagaaggg ggcactaaag gcatgttaca
     3121 aggtttagag atagttatag caatgttttg tataacattg atagtatcaa ttccattggg
     3181 gattggagtt gcatttttaa gattatcaaa gaataaatta gtaagtggta ttacacaatg
     3241 ttatatattg ataatgagag gtacaccttt acttttgcaa atgatagtca tattttacgg
     3301 attacctctt ttaggaattg tatttgacag atttacagct ggagtagtag ctttcttctt
     3361 aaattatgca gcatattttg cagaaatatt tagaggtgga atacagtcaa tcgatagagg
     3421 acaatatgaa gcttcaaaag tactaggatt tgataagttt actatgtata agagagtaat
     3481 ctttccacaa gtttttaaaa gaatactagc acctatttca aatgaagtaa tcacattagt
     3541 aaaagataca tctttagtat atatattagg attaaatgat attttaagaa tatctcaaat
     3601 agcaatgaac agggaagcaa gtctgttacc attatttgag gcaggagcga tatatcttat
     3661 atttgtagct atacttacta agggatttga attacttgaa aaaaaatatt cttattatag
     3721 ataaagttac aatattatag gaggaggttt tttatatgtt gaaaatcaaa aacttaaata
     3781 aatcatttaa aaaaaataga gtacttaaag atatttcttt tgagttagaa gaaggtcaga
     3841 ttggagtttt acttggtaag tctggtgcag gaaagaccac aatattaagg tgtataaatg
     3901 gtcttgaaga atttgatagt ggagagatta taatagataa tgaggtaata aaaaataaga
     3961 gagatatggc aaaaatacga ggaaaaatag gtatggtttt tcaaaatttc aacttatttc
     4021 ctcatatgac agtccttgaa aatataattg aatccccagt taatgttttt aaagttccta
     4081 gaaaagaggc tgaagagagg gctagagaac ttttaagatt agttgattta gaagataaat
     4141 taaattctta tccatttgaa ttgtcaggag gacagcaaca gagagttgct atagctaggt
     4201 catgtgcatt gatgccaaaa gtcctttgtt ttgatgaacc tacttctgca cttgatattg
     4261 atactattca aagagtagtc aatataatga acagacttaa agacaaaggt atgacaatat
     4321 taataattac acatgatgtt gtcttctcaa ataatgttgc agataaaatt ataagtataa
     4381 aagatggtat agtagaaaat gttcaaataa aagaaaaaat tgtttagagt tttataaata
     4441 taaactaggg tagaatttta taatataaat aaagggcaag taaaatcaaa tatactatgc
     4501 ccttttgtta tctcaaaatt taatcttata ttttatgttt aagttacatt ataatttata
     4561 ataaaaataa aaggactatt taaatatata atgtgaataa atgtttaata atttgaaata
     4621 tgataatttt ttatgttgaa agcttataat gattatgata taaattggtt atagtataca
     4681 atttatgggt ataacaataa aaaattagaa aggaattttt ataaaatgat taaattatat
     4741 ggatatacaa aatgctctac tgttaaaaaa gctaaaaatt ggcttaagga aaataattta
     4801 gaatttgaag atatagatat ggttcaaaac cctcctagta aagaggagtt aaaatcaatt
     4861 tataagacta gtggatatga tataaagaaa ttttttaata caagtggtat gaaatataga
     4921 gaattgggtc ttaaagatat tgtaaaaact gaatctgatg ataaactttt agaaatattg
     4981 gtaagtgatg gtatgcttat taaaagacca ttacttttgg atggtaaaaa tgtattattg
     5041 ggctttaaag aagatgtatg gaaaagtact ttgctaaaag aagattagga agagataggt
     5101 gtgttgatta aaattattaa ttaatacacc tataaattta tgtattaaat ctaattttta
     5161 taaatattaa ttagcaaatt caaatcaaaa aaattcatat caacaaacat attactt
//
LOCUS       DACXMZ010000241         5202 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.242, whole genome shotgun sequence.
ACCESSION   DACXMZ010000241 DACXMZ010000000
VERSION     DACXMZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.242"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            767..1300
                     /locus_tag="JD016_17020"
     CDS             767..1300
                     /locus_tag="JD016_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU5001319.1"
                     /translation="MDSKVIMVETDRLILRRYIESDLQDLFEYLSNSEVLEFEPYKPM
                     TIEEVKDSLEWRISTDEMIAVELKSTNKMIGNVYLGKRDFNSLEIGFVFNKNYWKKGY
                     AKESCEKLIELAFQSGIHRIYANCDPDNKNSWGLLEKMGFVQEAHLKKNVFFWRDNNN
                     QPIWKDTFIYSKLNSPK"
     gene            1531..2439
                     /gene="trxB"
                     /locus_tag="JD016_17025"
     CDS             1531..2439
                     /gene="trxB"
                     /locus_tag="JD016_17025"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAU5001320.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(2529..4352)
                     /gene="typA"
                     /locus_tag="JD016_17030"
     CDS             complement(2529..4352)
                     /gene="typA"
                     /locus_tag="JD016_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAU5001321.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            4567..4806
                     /locus_tag="JD016_17035"
     CDS             4567..4806
                     /locus_tag="JD016_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001322.1"
                     /translation="MEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIED
                     VLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            4954..5145
                     /locus_tag="JD016_17040"
     CDS             4954..5145
                     /locus_tag="JD016_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HAU5001323.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         1941 a          623 c          845 g         1793 t
ORIGIN      
        1 ttgtaatcaa attgagtatt agaaaaatat ttttttaact tttttaaaag tttaaaaacc
       61 tagattttca tagtgatgaa gctgttttta atgaaagtaa tcaaaagata actattaaat
      121 atctgtaata ttacaatttt atactacact atattacaaa aaaagtttac aaaactttaa
      181 tttgaaaaat gtaattatat ggtatatcat ataaatataa gtaaatagtg ctaatattta
      241 aactattaat cataattggt tcaacaaaag tatatagtta ataatgttta atttgaagaa
      301 attgttagtg tgaaattaca tatatattca aattaaattc ataaatattt cacataataa
      361 tagtatactg taaacaaaag gaggtattaa atatgggaat agtacttata acagcagcat
      421 tagttttggt tatgtatgga ctttcaattc tatcatatat atcagaaagc aatatattag
      481 aagacactgt aaatgataac caataaggtt ttgaattata taaataagtg agaactgcaa
      541 ataaaagttt atttttattt gcagtttttt tatatatatg taaaataaag ttaattaata
      601 aaaaataatg cttatattga aataaaggtt tattaaaatt tttatgatga tatgttcaca
      661 tttacctgat agacatttag atattaggtt taagaaaata tagtgtgttg tacatataag
      721 ctgaataata tttagtaaaa aaattaaagt ttgaagggga agtgtaatgg atagtaaagt
      781 gataatggta gaaactgata gattaatttt aagaagatat atagaaagtg atttacagga
      841 tttgtttgaa tacttgtcaa attctgaagt tctagagttt gaaccatata aacctatgac
      901 aattgaagaa gtaaaagata gtttggagtg gagaatttca actgatgaaa tgatagcagt
      961 tgaactgaaa tctactaata aaatgattgg taatgtatat cttggaaaaa gggattttaa
     1021 ttcgttggaa attggattcg tttttaataa aaactattgg aaaaagggat atgcaaagga
     1081 aagctgtgaa aagttaattg aattagcatt tcagagcgga atccatagaa tttatgctaa
     1141 ttgtgaccca gataataaaa attcttgggg gttattggaa aaaatgggtt ttgttcaaga
     1201 agctcatctg aagaagaatg tatttttctg gagagataat aataatcagc cgatttggaa
     1261 agatacattc atttattcaa aattgaatag cccaaaatag atgaacttaa gtttggtaag
     1321 tttatttatg tcagtttaaa gtatataatt tcatagttta agaatgaaaa atagcatttt
     1381 acagtgtagt aattaacttt taaattaata ctttttgtac actaataaaa aataatgttt
     1441 tgtttatagt atatatggat aataatagta aagtataaga atgatgagaa taatatataa
     1501 gaaataataa tataaagaga ggtaaacgtt atggtagata tcattgtaat tggtgctgga
     1561 ccagcaggtt taacttcagc aatttatgct atgagagcag ggttaagcgt tacagtattt
     1621 gaaaaaaata tttatggagg tcaagttgca agtacctctg aggtagaaaa ttatccagca
     1681 gttcaaaaga tttctggggt agaattttct aataatatat acaatcaagc agttgctcaa
     1741 ggtgttgata ttcagtttga tgaggttgaa gagataaatt tagagggaaa agtaaaggtt
     1801 gtaaaaactt cttcaggtga acataaagca aaggctgtta ttcttgcaaa tggagttgaa
     1861 agacgtaaat taggttgtgc aggggaacaa gaatttacag gtagaggagt atcttactgt
     1921 gcaacatgtg atggagcctt ttttaaagat aaagaagttg ctatagtagg tggaggaaat
     1981 actgcactag aagatgcttt atttttagct aataattgta caaaagtgta tttaattcac
     2041 agaagagata gttttagagg tgaagaagtg ttggagaaat ctgtgaaagc aagagagaat
     2101 attgagatac tttacagtca tggtgtagaa aaaatagaag gagaaaagac tgtatcgaaa
     2161 atagaagtaa aaaacttaaa gactgaagaa aaaagaacta tagatgttag tggtattttt
     2221 attgctatag gtcttaaacc taataataaa atgtttgaaa atgttcttga tttggatgaa
     2281 ggtggatata taatttcaga tgagtcttgt acaacatcag tagaaggtgt atatgtagca
     2341 ggagatagta gaactaagtt tttacgtcaa ataataacag cagcatcaga tggagctatt
     2401 gcagccgttc aagcagctaa ttatataaat gttgaataat ataaatttca aataaaaata
     2461 gccatataaa ctgaaataat ttttcagtac tatatggcta ttttgttaat tagaggtaaa
     2521 tgtacgtttt atttaccttg tctagatttg ttatatctta atctatcatg ttcatttaag
     2581 aatctttttc taagtcttat agaatcaggt gtaatttcaa ctaattcatc atcttctata
     2641 aactctagtg cttcttcaag agtaaatatt ctaggaggag ataattttat agcatcatct
     2701 gaaccagaag cacgtacgtt actcattttc ttgtttttac aagggttgac taccatatca
     2761 tcttttctgg aattcatacc aattatcata ccttcgtaaa cttctacacc agggtctaca
     2821 aacattacag ctctatcact taaggcatta agagaatacc ccatagtaac accaggtcct
     2881 tgtgctatta aaacaccatt tcctcttgaa ggtatttctc ctttaaattc ttcaaatttt
     2941 tcaaatgaac gaactaaagt accttctcca cgagtagcgt ttataaattc acttcgatat
     3001 cctaaaagac cacgagtagg tgctaagaac tctattttta catagtctcc ttctattgac
     3061 atagactcca tcataccttt tctcatgttt aattcattta taatagtacc agagtaaact
     3121 tcaggacagt ttacaacaac tctttctatt ggttccatta attttccatc ttctttatgc
     3181 attaaaactt caggtttaga aacaccaact tcataacctt cacgtctcat attttcaagt
     3241 agtatagata agtgaagctc tccacgtcca gaaactttgt atccatcagt agtatcaaga
     3301 ggttcaactt taagaccaac atttacttca agttcctttt caagtctatc tttcaaatgt
     3361 ctagttgtaa cgaattttcc actttttcca acgaatggag agtcgtttac taagaagttc
     3421 atagaaagag taggctcttc tatttttatc atttccatag gtagtggact atctaaatcg
     3481 catatagttt cacctataga tatatcagga ataccagcta taacaactat ttctccacta
     3541 gtagcttcat caacttcaac ttgttttaaa ccttcataca cagaaagttt tgatactttt
     3601 cctttagata caacagaatc ttctctacat atagcaactt gtgtattgtt tttaagagta
     3661 cctttgtaaa ttctacctat accaagtcta cctacataat catcatatgc aagagctgat
     3721 atttggaatt gtagaggttc attatcataa ttaggataag cttcaacgtg atttacaata
     3781 gttttaaata gaggagaaag gtcttcacta tcatcatcca tttcaagttt agcaatacct
     3841 tgtttagcaa taccatatat tataggaaat tcgcattgct catctgttgc atttaagtct
     3901 acaaataaat cgaaaacttc attaactact tcttccgctc tctggtcttt tttatcaatc
     3961 ttgtttataa ataatattgg ttttaaaccg aactctaaag atttttgtaa aacgaatcta
     4021 gtttgaggca ttgggccttc actagcatca actaatagaa taactgtgtc aacagttttc
     4081 ataacacgtt caacttcaga agaaaagtca ctgtgtcctg gagtatcaac tatgtttatt
     4141 ttgtaatctt catagtttat tgcacagttt tttgaatata tagttatgcc tctttctttt
     4201 tcaaggtcgt tactgtccat aacgcagtct ttaactactt cgttctttct aaaaacaccg
     4261 ctttgagaaa ggaaagcatc aaccaatgtt gattttccag cgtcaacgtg ggctataact
     4321 gcgatattta taattttatg tttttgtgac atttttgttg ctcccttcga ttaaaataac
     4381 ttattaatta tagcacaata tattattagg tacaaggagg aatatattaa attgtattaa
     4441 tatagtttaa attggataaa actgttagtt ttgcatctga aaattattct gtttaaaaaa
     4501 attttttaga caaataataa ccttaataga taaaaagatt aataaaagag taaaggatga
     4561 tatatgttgg aagtaaccaa aataataatt aaatattttg caagtgaaag tgatactttt
     4621 gtaaaagaag aagtttgtaa ttttgaagaa cttcttaagt ggtctcataa atttattgaa
     4681 ggtaaaaaga tagaagatgt tttgtatata aagtctaaag aaaactataa agtaaatgtg
     4741 attaagtctt tagaaattaa ggaattggaa atagtgagaa aacaacagat accaccgtac
     4801 atgtagtatt aataatatat aaaattaata tgtaatactc gcaataggat ataaatggta
     4861 tatatggtaa taatttatat taaaaaaact tttataaagt ttatatttgg gtataaaagt
     4921 attaattaga cattaattag gaggaactta aatatgataa gagtatgtcc aaactgttca
     4981 aatgtggatg taaataagct aaaaactttg gttggagaag aaaatgtaaa gactggttgt
     5041 ataggtcagt gtagagcatt taaaaaagaa gctgttggaa taatagacaa tgagcttgaa
     5101 ataaaggaaa cagaagcaga gttttttgaa gcatgtaaag aataaatttt aaataaaata
     5161 taaattataa taaatagagc atctaaattg tataagcatt aa
//
LOCUS       DACXMZ010000242         5146 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.243, whole genome shotgun sequence.
ACCESSION   DACXMZ010000242 DACXMZ010000000
VERSION     DACXMZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.243"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            65..1528
                     /locus_tag="JD016_17045"
     CDS             65..1528
                     /locus_tag="JD016_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HAU5001324.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1584..2720
                     /locus_tag="JD016_17050"
     CDS             1584..2720
                     /locus_tag="JD016_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAU5001325.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            2956..4071
                     /locus_tag="JD016_17055"
     CDS             2956..4071
                     /locus_tag="JD016_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAU5001326.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEILEGQINKSNVVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4223..5092
                     /locus_tag="JD016_17060"
     CDS             4223..5092
                     /locus_tag="JD016_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAU5001327.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         1974 a          538 c          983 g         1651 t
ORIGIN      
        1 aaatattgaa atatagctgt ttaaattatt gtctataaat ataaaatgaa tggtggtgag
       61 ttaagtgacc gatttagtta acaccattaa tggaattgtt tggtcacccg tattagttgc
      121 aatgtgtttg tgtataggat tatatttttc attgaaatta aaattttttc aaataagaga
      181 tgtaaaagaa atgtttactc tcttgcttga aggagatagt tctcatgttg gtatatcatc
      241 atttcaaggt tttgctacat ctatggctgg aagaataggg actggaaata tagctggtgt
      301 agctgtagca atagcaatgg gaggtccagg agcattagta tggatgtgta taatggctac
      361 attaggttca gcaactgcat ttgtagaatc tactctagct caaatatata aagatgagca
      421 tgatgggcag tatagaggag gacctccata ttattttgaa aaaggtcttg ggtggaaaaa
      481 gtgttcagtt ttattttcaa tagttgcaat aataagttat acatttttta tgcctggtac
      541 acaatcaaac actttatatc tagcaatgaa tcaagcattt ggattttcaa aaatgacaat
      601 agcagtagca tcttctattg cattagcgat aataattttt ggaggaatta aaaggattgg
      661 tttatttgct gaaaaagtag tacctataat gggtggagca taccttatta tgacattagt
      721 aataattata acaaatatta gtttagttcc agaggctatt gtaacaatgt ttaaatctgc
      781 atttggattt ggttcagttt ttggaggaat gctaggttca gcaatatctt ggggagtaaa
      841 aagaggtata tactcaaatg aagcaggaga aggttctggg acttatggtg cggcagcagc
      901 agaagtaagt catccagcca aacaaggatt agttcaagca ttttctgttt atgtagacac
      961 attattaata tgtatggcta caggtattat gattgtaata acaggaatgt ataatgttac
     1021 aggtgtttgc acagcactgc caggtgtaga accgggacca caatatgtac agagtgcaat
     1081 aaatacatta catccacaat taggtgatat ttttatagca atagttttat tattatttac
     1141 atttacaact ttattagcct tctcatatat gatggagact aatgtatctt acttagtaac
     1201 tagaattaaa gcagaaaaac atgaaaagta cataattaat gtctgtagat ttatgcttat
     1261 agcatgtgca gcaatgtgtt gttttataga accacttaca agctgggcat taggtgatat
     1321 atgtataggt gtattaacat atataaattt aattgccatc tttacactaa aaaaaccagc
     1381 tataaaggca tattatgatt atataagaca gaagaagatg ggaataaaaa ggagtgatag
     1441 aacttttaac cctatagagt tagatataag aaatgctgag ttttgggaaa atagatactt
     1501 aaaagaggat aagcatgagt taaattaaag ttataataaa atataaaaat tactaaataa
     1561 atttagtggg aggtaagatt ggtatgaaag aaagaataat acaagtggct gattcaaaga
     1621 aagaaaaaat tctagggcta tgtcaatctt tatatgatga accagaaatt gctttgcaag
     1681 aatataaatc tgctaaaaaa atatcagaat ttctaagaga agagggtttt gatgttgagg
     1741 aaaacttggc aggtatggct acagctttta aagcaaccaa gaaaaatgga gatggtccta
     1801 aaatagcatt tatagcagaa tatgatgctc ttccaggcaa tggacatgca tgtggacatc
     1861 atttaattgc atctatggga gttggagctg gaatagctct ttcaagtata ctggatactt
     1921 acaagggaga agtaagtata attggaacac ctgctgaaga aactggagat ggaaagcctt
     1981 acttgataga acatggtgta tttgatggct atgatgcagc aatgatgata catccaaatt
     2041 caaaaacttg tgtaacacct gaaattatag caataggagg tctagatttc atatttactg
     2101 gaaaagcatc tcatgcagga gctaagccat ataacggaat aaatgcttta gatgcagtag
     2161 tactactata taataatata aatgcactaa gacaacaatt agtagatgga acgagaatac
     2221 atggtattat actagaagca ggaactgctg caaatgtaat acctgatatg ggaaaagtaa
     2281 gattagagat aagggcaaaa gaacaaaatt actttgatga agtagtagaa aaagtgaaaa
     2341 attgtgctag aggtgctgca atagccactg gatgtgaact tgaattttat cactttgaac
     2401 caacttgcca aggattaaat gaaaataaag ttttggtaga catatttaca aagattatgg
     2461 aggaatttgg aatatatgaa gatgaacaag taatgttagg ttcaacagat atgggaaatt
     2521 taagtcaaat aatgccatgt atacatcctt tgatgaagtt ttctgaaaat ggagaagaac
     2581 tacatacaaa agaatttttg gaggcttcta taaattcata tgcaaaagat agagtaattg
     2641 atggtataaa aatattagct ttaacaggat ttaatttatt tgaaaatcca gaattattga
     2701 aaaaaatgaa ggaagagtag aacttagact atttttaatt atactcttta aaataagaag
     2761 agtgtctcaa aatgaacttt ttagtttatg agacactctt ttttgtatga aattaaatat
     2821 atttttatta ttttaaacat gaaaaagagg cttatctgtt tttatatgac cttttttaac
     2881 ttgtagcaca aaatatcatg aatgattaca attcgaatga taaaatcgat tataagatat
     2941 ataggagggg tacatatgga aactttagaa gtaagaaata ttgaattgag tggtacaaat
     3001 tatgagatag gatacagact aggagaattg gttgctaata tgccagagat actagaaggt
     3061 cagattaata aatctaatgt ggtaagcaaa aaagaagaaa aagagatgat agagttgttt
     3121 gataaatatt gtccaggtct taatgaagaa ttgcaaggtt ttgctgatgc aattcaagtg
     3181 aattgtaatc aaattttata ttatactatg acttatctta aaccaggttg tagtcaagtt
     3241 gcattagcac cagaattaac tgaaaatggg catgtactat ttgcacgtaa tttcgatttc
     3301 tcacacaata tggaggattt tgttttatgt aaaactaagg taaatggaaa atatgcacat
     3361 ataggtacta ccgttatgca atttggaaga ggtgaaggta tgaatgaatg tggcttaggt
     3421 gtaagtcagt catcatgtgg tattcctgta ggcaattcag atggtttaag aaaaccagca
     3481 attgtaggtt tacagttttg ggctgttatt agatatttgt tagaaaattg caaagatgta
     3541 gatgaagcat tagaatattt aaaagatatg ccaattgcat acaatattaa tttattgctt
     3601 gcagataagt ctggaaacat tgcacttgtg gagactttag atgggaaaaa agaagtaaat
     3661 ataatcaata gtttagaaag taaaagagaa tattttttac actctactaa tcatacacat
     3721 attgataaac ttcataaatt agagccacaa tcaatgaaaa attctattca tagatataaa
     3781 ttaattaaag aatatataaa taagtctaaa aaaattggag agaaagagtt aatgaattta
     3841 ttgtcttcaa aatatccaaa tggcttatct tgtaattact ataatgattt ttttggaaca
     3901 ttaaaaagta tagttatgga tttaaatata ggaaagttta atattttatg gggtggacta
     3961 gagaataaat gggagagcta ctatttaaaa aatgacatta aatctataac acagaggata
     4021 aacataaata ttgaaaaagc accatctgac ttttttgatt tcattgaata aaaaatgtat
     4081 ttaattattc taaataatta ggatatataa atcaactaaa agtttttatt tatcgtattg
     4141 aattagttat aatattttag tatagtaaaa aatagtgcta ttttacatat ttaaagagtt
     4201 taagtagaag agaggtgcca tattggatta cttaaagtat tttgaaaaag ctgtaatgta
     4261 tattgagaat aatttacatg aaaatataac tgtaaatgat gtggcaaaag aaactggata
     4321 ttcttattat catttaacac gcctatttaa gtctatgttt ggtgagagtg taggaagtta
     4381 tataaaaaaa agacgattag taagtagtac aaaagaactt ttatatagtg ataaaaaagt
     4441 gatagatata gctattggta gtggatttga atcttcggaa gcatttagca gagcatttaa
     4501 atctatatac aaggttagcc ctatagaata tagaaaaaat agaattgatg tatttgttgg
     4561 taaaaagaaa aaattagagt tagattttat gagacatctt gttggaaata taacgattaa
     4621 accagttata aaagaaatag aaaaaataaa ggtaataggt ataaaggata aagtaatttt
     4681 agaaaataat tcattacctg acttgtggga gaaattcaga aaagtacatc atattgtccc
     4741 aaatactttg ccatcaaaga gagtttttgg aatctgtgaa gcaatctctg aaatacacct
     4801 agttagtgaa agtatggaat ttaatgagat aataggactt gaagttaata gttatgatgt
     4861 gattccaaat tcatttgttt caaaaactat taaggaagga aagtatgcta tatttactca
     4921 tactggtagc ttagataatt tagacaaaac ttatgaatat atatggggta cttggttttt
     4981 aagttcaaaa gaagaacttg atataagaga tgattttgaa gtatatgatg aacgattttt
     5041 aggtcctagt aatgtgaact cacaaattga tatttatata cctataaaat aatctataag
     5101 aaaactaaaa gataatttga ttaagaaata gatgttcgta aaagag
//
LOCUS       DACXMZ010000243         5120 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.244, whole genome shotgun sequence.
ACCESSION   DACXMZ010000243 DACXMZ010000000
VERSION     DACXMZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.244"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            119..1645
                     /locus_tag="JD016_17065"
     CDS             119..1645
                     /locus_tag="JD016_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAU5001328.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1667..2230
                     /locus_tag="JD016_17070"
     CDS             1667..2230
                     /locus_tag="JD016_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001329.1"
                     /translation="MYMNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGT
                     FILSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCI
                     VAFSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGL
                     EDTMIDMVDALIGTIITIPFIVKKSNI"
     gene            2752..3795
                     /locus_tag="JD016_17075"
     CDS             2752..3795
                     /locus_tag="JD016_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001330.1"
                     /translation="MLKKWFGIVKKRQKSESAKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            3906..5048
                     /locus_tag="JD016_17080"
     CDS             3906..5048
                     /locus_tag="JD016_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAU5001331.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKSTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2158 a          423 c          920 g         1619 t
ORIGIN      
        1 gaaaactaag tgatttttaa tttagctttt tattaagctt attttttata ctatttataa
       61 taaataaact ccatgatggg aaaactatta ctatttaata tgtaaaggag tattttaaat
      121 gaatgaatca tattggtttt taaactcaag tccaaaagaa tataacaaat taggtgaaaa
      181 tataaaaaca gattgtctta tagtaggtgg tggaataact ggattgacaa ctgcatattt
      241 attagcaaaa gaaggtaaaa aagtagtgct tgttgaggct gataaaatag gatatggaac
      301 ttctggaagg aatactggta aggtaacttg tcagcatgat atattttact ctaagataga
      361 gaaaaaatat ggattagata aagcaaaatc atattataat gcaaataatg aagctttaaa
      421 tttagttgaa caaataatag aagaaaacaa tataaaatgt gattttaaaa gggaaacttc
      481 ttttgtattt acagaaaaag aggatactat aaagaatatt aaagatgaat atcgtacatg
      541 taaaaaaata ggaataaatt gtgagtatca tgaaactata gaaaatattc cattagatat
      601 aaagggggct attagtttta ctaatcaagg tcaatttaac ccaaagaagt atattgatgg
      661 attggcaaaa gcagcagtaa atttagggct aaaaatatat gaaaatacac cagtagtaga
      721 ccttgaaaaa ggaaaaatat gtagggttaa aacaagagag gataatataa tagaagctga
      781 gaatgttata atttcatctc atagtccatg gtatgatgga ttgaattttt attttgcaaa
      841 agaatatgca gagcgtgctt atttaatggt tgcagttttg gaaaataaat tagcagatgg
      901 tatgtttata agtatagatg acccatcaat aacttttaga caatataatg atggaagtga
      961 aaatttactg atttttggag gaggagacca taaagttggt cagggaggaa ctgagaaaga
     1021 aatatttgat gatttagagc attatggtaa agaagtcttt aaggtaaaag attttaaggg
     1081 gaaatggtct gctcaagaca atatgagttt tgacaatgtt ccttatattg gttatatcaa
     1141 caaaagagaa gacaatattt atgtagctac agggttctct aaatggggaa ttactaatgg
     1201 aactgctgct ggaataataa taaaagattt gataataaat aataattctg attataaaga
     1261 tacttttaat ccatccagat taggtagcta cttttcaaaa gattttataa aggaaaatgc
     1321 aaacgtagca ataaattatg taagtggaaa attaaaaata ggtagtggag atatgcctaa
     1381 aaataatgga gaaggaaaga ttgttaatat tgatggaaaa agatatggtg tatataagga
     1441 tgataatgga gatttttata ttgtagacac tacatgtact catttaggat gtgaacttaa
     1501 ttttaacagt gaagaaaaaa catgggactg tccatgtcat ggttctagat ttgactataa
     1561 gggaaatata cttgaaggac cagctcttaa gccattaaaa ctttatggac atggtgataa
     1621 tgatgttaat cctaagttat tataaaatta tttttaggaa ggtaaagtgt atatgaataa
     1681 acttttaaag ccaataacta tactattgta tatagtttat attttatctg ccgtattttt
     1741 tgtagcaaac aaagaatatg gaagtctagg tctggtagca attagtttag ttggtacttt
     1801 cattttgagt tttataaata agcaaacgga aagattattg agtaaagaat tatatataat
     1861 gcttgcttta tttataatgt tttcatcatt gttaggtact tgttttaatt tttatgatat
     1921 aaattattat gatgattttt tgcatttatg gtcaggtata atatcttgta ttgtggcttt
     1981 ttctataatt aaatactttt atacacaaag agagataagt aaaatgagta aagtgttttt
     2041 agttatattt ttatttatgt ttagtatggg agtagcgagt ttatgggaaa taggtgaatt
     2101 tttaatggat acttttgttg ggactactac acaggccgga gggttagaag atactatgat
     2161 tgatatggta gatgctttaa taggcacaat tataactatt ccttttatag taaaaaaatc
     2221 taatatatag ttttaaattt tttatattaa ttataatgga tttaattaat catgttgagt
     2281 ttcaaggtct atatgattaa ttaaatccat ttttgttatt tataattatt attgaatata
     2341 tttttacata taagatataa ggtgatagat agaaattgtt ggattatgta aataattaat
     2401 atataatacc attatgggca ataaatacaa atcaaatgat aatatagtct atttatgtaa
     2461 atgttatatt gtatactaag tataatagta agttttttga tagtggtgta gaaagtaaaa
     2521 atctactagc ctaaattgat gtatggatta gtttaggaac agtaattgca tatcttaagt
     2581 ttgatgtcat gttgggctac tagaaatagg tcatcttttt cacttgaata tcttttcttt
     2641 aaacatggag agtatcatat caaaaacata tgaaaattaa gtgatttata agaacagtaa
     2701 tattttatat gatagtatag aatagtaata aagtattagg aggaacattg agtgttaaaa
     2761 aagtggtttg gtattgtgaa aaaaagacaa aaaagtgagt cagcaaaaga agaaggtgaa
     2821 gtaatattaa aaaacgaaaa aatattatct gaagaaaagt tgatagatga agaaggagtt
     2881 gtagttaata ttgataatga aatattaact aaagtagaag tagtaaatga tgataatgaa
     2941 atagaagaaa aaataataga agaagattgg ttaattagtg aaaataccat aaaattagat
     3001 gataaagaag caattattaa tgataaaaac atagaattat gtaaaaaaga agttcaagtt
     3061 gaaagtgaaa aaataggttt aaataagttt gaaggacttg accaaaatga aaattataat
     3121 ctagaaaaaa atgttattga agaaaaaaaa gtaagtgaat gtttgacaga agaagattta
     3181 gagtacataa aagaaattaa aataaaaaga ggaaaaagta taaaagctat aaacttgtat
     3241 actaaagaag agtgggtttt tgacactcat atacagtgta gtaaaaaact caaagttcca
     3301 ttagggtaca taagagaaaa tttaaaatat ggatatatgg attactttgg agatgcaata
     3361 aattatttaa gtgaagtatt aaatatagat gaatactgca aaagtgaatg gagctatctc
     3421 gataatagca aatctccatc tgagatattt aatattctaa acaataaaat atttagtata
     3481 aggctttcta atgaaaaaag aaatgaaatt ttgacaaatg ataaaattga agcattaaaa
     3541 atgaattata gatttgaatg tattgatgaa gaatatgatg aatattttaa aaaatataag
     3601 tctataatca aaagaggtgg aaagaaaaaa gttgaattag taaataaaaa aggtgacatt
     3661 ttagaaatat ttaagtcttt agaagaatgt gccatttatt tgcaaaagga aaagaatgaa
     3721 gttatacaaa tgttaaaata tggagataca aaagtaggaa gaaactttat aaggtatagt
     3781 ttgagaagta tttaatttca ttttaaattc ataagagttt catttctgat tcacacattt
     3841 aatattattt taatgtaaaa taataaaggt gaagtattgc acaattaaaa ttaaggaggg
     3901 tattaatgtc tgaagaaaaa aatccaaaag aaaatacatt agatgaatct aaaaattcag
     3961 ataatgtaaa ttctaaaaaa gatgatgtgt cagatgaaaa aagtactgat aacaaagtta
     4021 aaaaaaggga aataaagcta aacaacaaaa cacttaaagc tattgtagca attgttgcag
     4081 taggagctat atgtttaggt ggaggctatg tttttggcaa agaagctgga agaaaattgc
     4141 ctgctacttc aaaacactat aatagcagtg aggtagtagc tactattggg gataaaaaaa
     4201 taacaggtga aatgttaaga aagagaatgg agccattatt ttatttaaat gcaaaaacta
     4261 ctatgactga tgacgaaata cgttcatatg aatcaaatat gattgaaact atgattacat
     4321 ctgatatatt atcctctgaa gcagcaaaag agaaaataag tataactaat gaccaactag
     4381 atgctcagta taataattta atgagtagta tagaatcagc aatgggaatg actaaagaag
     4441 agtttttaaa acagtttgat ttgactgaaa aatatataaa agatgatatg aaaaaagagc
     4501 tagtagcatc aaaatattta aatgaaaaat caaaagttac tgataaagaa gcagagaact
     4561 attacaataa aaacaagagc aattatttac aagttagagc ttcacatatt ttaataaaaa
     4621 ctgttgatga taaaggtaag caagttagta gtagcaaaaa agctgaactt aagaaagaag
     4681 ctgaagaaat acttaaaaaa gcccaagctg gggaagattt tgctactcta gctaaaaaat
     4741 actctgaaga tagtagtgca gaatcaggtg gagatttagg tttctttggt aaaggtcaga
     4801 tggtggaatc ttttgaaaaa gcagcctttg cattgaagaa gggagaggta tctaacaaac
     4861 ttgtagaaag tgattatgga tatcatataa ttaagaaaac tgatgaaaaa tatcagccat
     4921 ttgaagaggt aaaatcagat ttagtaagca gtttaacatc agaaaaacaa aacctactta
     4981 tacaaaatct aaaagaaaaa tataatgtta aaattacaga taaatatcaa atgaaagcta
     5041 aagaatagat aaagtcaatt tattaaaaaa aagaaaaatt aactgtatgg ataaaagcta
     5101 tttttactct caatatagag
//
LOCUS       DACXMZ010000244         5107 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.245, whole genome shotgun sequence.
ACCESSION   DACXMZ010000244 DACXMZ010000000
VERSION     DACXMZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.245"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(129..329)
                     /locus_tag="JD016_17085"
     CDS             complement(129..329)
                     /locus_tag="JD016_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAU5001332.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(554..730)
                     /locus_tag="JD016_17090"
     CDS             complement(554..730)
                     /locus_tag="JD016_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001333.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(784..873)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1053..1253)
                     /locus_tag="JD016_17095"
     CDS             complement(1053..1253)
                     /locus_tag="JD016_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5001334.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1255..1605)
                     /locus_tag="JD016_17100"
     CDS             complement(1255..1605)
                     /locus_tag="JD016_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HAU5001335.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1767..1956
                     /locus_tag="JD016_17105"
                     /pseudo
     CDS             1767..1956
                     /locus_tag="JD016_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2433..2603)
                     /locus_tag="JD016_17110"
     CDS             complement(2433..2603)
                     /locus_tag="JD016_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001336.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESRYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(2992..3480)
                     /locus_tag="JD016_17115"
     CDS             complement(2992..3480)
                     /locus_tag="JD016_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAU5001337.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSITIPNDKNMIITFLSNKS
                     DN"
     gene            4284..4658
                     /locus_tag="JD016_17120"
     CDS             4284..4658
                     /locus_tag="JD016_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAU5001338.1"
                     /translation="MKEEMLIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            4763..5107
                     /locus_tag="JD016_17125"
                     /pseudo
     CDS             4763..5107
                     /locus_tag="JD016_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088802.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         2053 a          630 c          586 g         1838 t
ORIGIN      
        1 tttataaaaa gtatttagac taagtattat ttttaagtat ttttttgttg aagttatttt
       61 ttcctttttt acaaaatgcg taaataaaaa aaagactctt aaaagagtct ttccgaaaaa
      121 tctatatatt ataatatagt tatattttct gcttgaggac ctctagcgcc tttaactata
      181 tcaaagctta ctttttgacc ttcttctaat gatttaaatc ctgaagtttg tatagctgag
      241 aaatgagcaa atacgtcatc tccactttcc attgatataa atccaaatcc tttttcatta
      301 ttaaaccatt ttactattcc attattcatt aaaaatactc ctattaacct atatcaagat
      361 atacttgatt tacttgtgta ttaccacaaa tactattgta tcatagatta acataaatgt
      421 ctatttaatt ataattttta gttaacattt ttatttattc ttaatatgaa aataaattta
      481 aacattttat attaccaata tattaatgta aatgtagata aaactaaaaa gggttttcta
      541 gaaaaaaatc tgtctaatta tacttactta ccaataccat cacttgatgc aagcaagatt
      601 ccacaacttt ttctccgtac tctaatataa aatcatctaa ctgtgtatct ataaaatcac
      661 aataatcata tgaactacca atataagcag ttaacatatg tgtacattta tccattttat
      721 ctctaaacat aatgtattta tccccctaaa aaactttcat gaaaaacaaa aagaaaatat
      781 ctttttggca actggctgac ataacttatt ttataagcgt tagtccctat agctttgcgt
      841 cactaaattt ctctagtttt gccgatttag ttttattcta cagattaata atacaataga
      901 attagttatt attcaacata tctagctgaa aaattaataa atttatatat gaacgttaaa
      961 ttatctgaaa aaataaaaag cccctctgtc aggagtgact taaaaattga atttaagttt
     1021 gtatttaaaa cacattttta ataatgcaaa aactaatcaa aaataaacaa ttcatcaata
     1081 ggtgtatcta ctatttttga tatatctatt gctaatttca aagaaggatt atactttcct
     1141 gcttctaaac gcataatggt ttctcttcta actcctactt tttctgctaa ttgttcttga
     1201 gttaattctt ttaattgtct atattttttt aggttacaag tgaatttagg catattattt
     1261 ttcctctaaa atataaagtt ttatagcata aacatttaaa gtgattgcat atgtaattga
     1321 tagtaaaaca cacattaatt ctatattcct agacactata gtaatacacc ataaaatgaa
     1381 taattctata atagcaagct gtcctgtaaa tgccatagct gctttttcat cttgtacata
     1441 cctttcatct gctttatcac catttatttt tgctataata aagtttgaga aaaaagcaaa
     1501 aaatccaata tatgtaaggt cagcaacatt gccagatgaa aaatatctaa gacttaaaaa
     1561 tccaaggaaa ccacaaaaac ccataaaaaa ttttttctga atcatttttt atcctcctta
     1621 gtaattttaa aagagatata tttatatctt tatgatacaa atatatctct tttatattct
     1681 tttgtcaata gaaaatcagt attatacaat atttgtactg caccataatt gttagagcaa
     1741 aaatctactg cttgagatgt ttatgtatga ctaaatatga ttttaatttc aaattaaaag
     1801 tagctaaatt ttatttgaat agagaaggaa gagatatttt aatagtaaaa caatatggag
     1861 tttataacca ttctcaagtt gaaagatggg ttaatatatt aggagagtgt gcactaaaaa
     1921 taaaaaaaat tttctatact atatggttca agttagatgt tataaactat atgatgataa
     1981 ctaaatatag tgtacaataa atcgctaagt attttggaat aaacaatagc acagttatag
     2041 caacatgaaa ataaaaattt ttacatagtg gtgttgatgc cttttctaaa atgaaaggaa
     2101 gaccataatg aataaaaaca aaaacactaa agaaaaaata aattaacaag agaacaagaa
     2161 ttagaatatg aaaataaatt tttaaaagat gagcttgagt ttttaaaaaa gatgtgtgtc
     2221 tcagggaagc cacattaaaa aacagtggta tcattattaa tcaaaagaaa gttagatgta
     2281 ttatcaaaaa attagaactt aaatgcatag cattttttca taaatcacat aaatataact
     2341 cctataaagg tataattgga aaagtaaaaa ataataaaac aaatagaaga tttaaaacat
     2401 caatagcata tcagaaaata acaaatgata tgctagaatt tataaaatat atctaatgat
     2461 aaaaatggaa aatcaattat aaaaaaggca tatctcgatt cattttttgt tatatagtca
     2521 aataagaata ctatagatga aagatacgat tttgcatatg tattaagtaa gtattctgat
     2581 gtttttggtt gatacacatg caataaagaa gttgtgtttg cagataaatt agcaaaacta
     2641 tagtataagt tgcaacaata aagaaaatga aaattatcat actaaagcat aaggcatgtt
     2701 acaattttat tagataggac attaacatac actaaagtac tttttattat aaagagtact
     2761 tatttttttg aaattcatta atgtataaac tatcaagaat attactcaac ataatttaaa
     2821 aacaacttga ataggtattt ttaaattatg tgatataata aaaaatagaa gtgtatgcca
     2881 atacacttct gtagttataa ctaagcattc tattcttgag gagtgctttt ttcattttta
     2941 taaccaactc tttttacttt aacaataatt atcagaccta tatatttatt tttaattgtc
     3001 tgatttatta gataaaaaag ttattatcat atttttatca tttggtattg ttattgaact
     3061 accataatct tcttctaatt tatctagacc acttttactt atttctccat ctataattat
     3121 gtttaattct aagtattctt cactaccatt agatgacact aaagtgtacg aaccattata
     3181 aggcatgcct aaattttcta ttttatgtgc caaagggtct tcatcaccaa taccagagtt
     3241 tactgttatt tttccatctt ttttagtcca ccatattatg tttccttcag tatattttga
     3301 ttcatctgca tagtagctaa gagacgcact ttttattgca cttatatcag cttcaagttt
     3361 agctatcttt gctttttcta tatttttaaa taatgctgga agtgctacta ttgccaatat
     3421 tcctattata gcaattacta ctaataattc cactagagta aatccttttt tatttttcat
     3481 ttatttcccc tccatataaa attaataata tataaatttt tattttgttg atttctactt
     3541 tctatatttt accatatata gacaaaaaaa tcactttcta tgacaaatat actattattt
     3601 tacaatttat ggaaatgatt taaaatgaca tttcttgtta gaaaagatat aagaaatgga
     3661 ttaaaataaa gaaaactaca gtcatatcaa tgtcaataat attatttaaa agcagatgaa
     3721 aataaaaaga ggactcagaa aataatgtct atttaattat acttacttac caataccata
     3781 acttgatata aacaagatta tactacattt ttttatgctc tagtataaaa tcatctaact
     3841 gtgtatctat aaaatcaaaa taattatatg aactatcagt ataataagtc aaaatatgta
     3901 tacatgaata catattttaa aagatatata ttattacata cctaaaaaac tttcataaaa
     3961 acaaaaataa aatatgcttt tagtaacttg ttaacataac ttataattta ttttataagc
     4021 attagcctat atagctttgt gctactaaat ctctttgatt ctaacgattt agtgctattc
     4081 tacaactaaa ataatacaat agagctattt attgtttggt agcattgttt gtaaattaaa
     4141 caaatttata catatgaata ttattttaca ttcaaaaaaa cagactaaat atgtagaata
     4201 tttggtaaaa cttacctcta gtttttgtct taaaaagctc ctattattaa cttaagttag
     4261 aagaattaag gaagtgacat aaattgaaag aagaaatgtt aatagaaaaa ttattaagag
     4321 cagaattaat agtaatggag tatttatgga aaaaagacac tctaatgcct aaaaaagaaa
     4381 ttgtaaaaga aataaaacag atttatggat ggcataaaag tacaataaaa atactactaa
     4441 aaaggctagt tgctaaaagg tatttggcta gatatattat aaattctcaa tctcattata
     4501 aaataataga tagtaaagaa tattatactt ttaagaaaaa ggtactaaaa tctagtaaaa
     4561 gcataaaaat aatgcgttca cttaccacta cacataaaaa tgtaagtaaa gagaagttag
     4621 acctattaga tgagtattat agaaacttaa aagaatgata tagtaaaaat cgaatgtaat
     4681 tatttacctt aattcttttg tttgtaaaac tttattgata cagaatttaa catatacata
     4741 aattggtaaa ggaatgtata ctatgaaaat tagtaaatta ccagaagcgg aattaaaagt
     4801 tatgagatat atttgggaat cagaaaaggt attaatatca agagaaatca ccaagtctat
     4861 ggaacaaaaa tatgaatgga aagatacaac tatatttaca gtattaaaaa gattactaaa
     4921 gaagagagtt tttagaagcg gagaaaatag ataagtgtac acactataat gtattagtaa
     4981 aaaaataata tcagagattt taaacaaaag aatttttaaa gaatacacat aaaaattcaa
     5041 tattaagttt aatgtcagca ctatgtagta aggatgatga agtagatggt gatattttaa
     5101 atttatt
//
LOCUS       DACXMZ010000245         5043 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.246, whole genome shotgun sequence.
ACCESSION   DACXMZ010000245 DACXMZ010000000
VERSION     DACXMZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.246"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            31..1914
                     /locus_tag="JD016_17130"
     CDS             31..1914
                     /locus_tag="JD016_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAU5001339.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKESILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGDFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2138..2818
                     /gene="pgmB"
                     /locus_tag="JD016_17135"
     CDS             2138..2818
                     /gene="pgmB"
                     /locus_tag="JD016_17135"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAU5001340.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2815..>5043
                     /locus_tag="JD016_17140"
     CDS             2815..>5043
                     /locus_tag="JD016_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAU5001341.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHIKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIK"
BASE COUNT         2082 a          455 c          957 g         1549 t
ORIGIN      
        1 taaattatta taaaggaggg aaattttaaa atgtctaata aaaataaaga aaatataact
       61 ttaagtaatc aaataaaagg aacaattcaa aatagtttaa caaaagaatc tatattacat
      121 ataccaatga gcaattatgc ctatggttat gatagagaaa cactacatat aagacttaga
      181 actaaaaaaa atgaagcgaa aagagtaatt ttacgaatag gagaccaata tgtgtgggat
      241 aaaggaggtg caggaggagg aaatttaaat gcatctggag ttggttggtc tggtggaaca
      301 aatatagtaa tgagtaaaga agtagaaact gaattatttg actattggat agctaagtgt
      361 aagcctttaa ataaacgctc acgatatggc tttataatag aaggtcaaga ggaaaaactt
      421 ttatttacag aaaaaagaat aatagagttg ggaaatgaaa atgatgaaaa ggaattatgt
      481 gaaataggtg atttttttgg atatccatat ttaaattata tagatatacc taaagtgcca
      541 aattgggtga aagatactgt ttggtatcag atatttccag atagatttgc aaatggtgac
      601 ccgtctataa acccagaagg agtagataaa tggggagcta ttccaaccag agataatttt
      661 acaggtggag atttacaagg tgtgatagaa cacttagatt atttgagtga tttgggaata
      721 aacggaattt acttttgccc tattacaatt ggaaaaacca atcatagata tgatacggta
      781 gattatatgg aggtagaccc tacattagga gataaagaga ctttaaaaaa actaatagaa
      841 gaagcgcata agagaaatat aaaaataatg ttggatgctg tttttaatca tataggatat
      901 tattctaaac agtggcaaga tgtagttcaa aataaggaaa attctcgata taaaaactgg
      961 ttttatataa aagatatgag taaaattgat actccaatag aacaaataga tgaaaaaaat
     1021 ataccttatg aaacctttgg gtgtgaaaaa tatatgccta aacttaatac agaaaattca
     1081 gaagtaataa aatatttatt ggatgtaggc aagtactgga tacaggaatt tgatatagat
     1141 gcgtggagat tagatgtttc aaatgaagta gaccatgttt tttggagaaa gtttagagaa
     1201 gaagtcaaaa aagtaaaacc agacatttat atattgggag aaatttggca tggtagttta
     1261 ccatggttaa tgggtgacca atttgattca gttatgaatt acttgatgtc agaagccatg
     1321 aaaaaattct tttgtacaga tgagataaac gccgaagaat ttaagtatat gataaatgat
     1381 gtaatggtaa gttatccaat acaagttagt gaagttattt ttaatttact tggaagtcac
     1441 gatactacaa gaatattgac ttatgctaat ggaaatattg atagatttaa acttgcatat
     1501 atgtttatgt ttgtacaaac aggatgtcct tgtatatact atggtgatga aatcggaatg
     1561 gaaggagaat taagttcaat atctgaaggt caaagaaaat gtatggaatg gaatgaagaa
     1621 aaatggaata aggatatatt tgattttatg aagaagatta ttagacttcg taaagaaaat
     1681 aaagaactta ggtctatatc aaatgaatgg gtgttagcag ataaggaaaa tggaacgatt
     1741 atattgaaaa aagaatctat ttctataata ataaataatt catctaaaaa tataattttg
     1801 acgttaccag attatttagc aaataaaaaa gtaaaagatt tatatgaaga agaaatagtt
     1861 gatttaaaag aagaactaat gttagagaaa tacaagttca ttattttaaa ataatacaaa
     1921 aaagtattta ttttagattt catgttaaat tattatacag tttaaacaag tctttataaa
     1981 tgataaaaaa tatggtctac aagtgagtga attgaaagta gattttaaat taaagactga
     2041 gttagagtag ataattttat aaataatatc ttaaatacaa gatagaaaat aaattgatta
     2101 ttagtctaaa aatttcttgt agaaagagga aataagaatg attgaagcat ttatatttga
     2161 tttagatggt gtaattacag atactgcata ttatcactat atggcttgga gaaaactagc
     2221 tcataaagtt gggatagata tagacacaaa atttaatgaa tctttaaaag gcataagtag
     2281 aatggagtca ttggacagga tattagaatt tggaaataag aaatattcat tttcagaaga
     2341 agaaaaagtg agaatggctg aagaaaaaaa taattactat gtatcattga tagatgagat
     2401 aacttcaaat gatattttgc caggcataga aagcttgtta attgatgtaa aatctaataa
     2461 tataaagata ggtctatctt ctgcaagtaa gaatgcaata aatgtattaa atcatttggg
     2521 tataagtgac aaatttgatt ttatagctga tgcaggaaaa tgtaaaaaca ataaaccaca
     2581 tccagagata tttcttatgt cagcgaaagg gcttaatgta aatccacaaa attgcatagg
     2641 aatagaggat gcaagtgcag gtatagacgc tataaactca gctaatatgt tttctgttgg
     2701 tgttggaaat tatgaaaact taaaaaaagc aaatttagtt gtagattcaa caaatcaatt
     2761 aaagtttgaa tatatacaag aaaagtataa tgagtatata gttaggagaa ttatatgaaa
     2821 tgtttattta gtaatgtaga tgagtggaag attatacaag ataagattga aatcaaagaa
     2881 aatagattag cagagtccat aatgagtata ggcaatggat atatgggtat gagaggaaac
     2941 tatgaggaaa attatagtgg agatacacac aaggggtctt atatagctgg agtatggttt
     3001 ccagacaaaa ctcgtgttgg ttggtggaaa aatgggtatc cagaatactt tggtaaaatt
     3061 cctaattcag tcaattatat aggtgtaaga attttcatag atgatgtaga attagattta
     3121 gcaaaatgta aagtagaaga tttttataga gaattagata tgaaaaatgg tattttaaat
     3181 cgtaggttta ttgtaaatat aaatgataaa aaatttgaag ttagggttgc taggttttta
     3241 agtatatctg ttaaggaact tgcagtaatt aaatacagta taaaatcatt aaattctaat
     3301 actaaaataa aatttgtacc atatttagat tcaaatataa aaaatgaaga ttcaaactat
     3361 gaggaaaagt tttggaatgg aatttcttca agttctttag aatataaagg tagtattctt
     3421 tctcgaacaa aggagaatgc atttaataca ccagtattca ctgtgggttc aatgatgtat
     3481 attaagacaa atggtgatgt agaagctgta tctcatgaga gtgattataa ttactgtgaa
     3541 aattcgataa caatggatgt tttaaaaaat gaggttgcaa gtatagaaaa atatgtatct
     3601 ataacctcaa ctagagatta taaggaaagt gagctattaa gaaaatgtga agacatttta
     3661 aatagagaac tatctaaaag ttatgatgat atattaaatg aacatataaa gttatggaat
     3721 aaaagatggg aaagtgcaga tgtaaaaata aatggagata aaagtagcca acaaggtata
     3781 agatataatt tattccaact attttcaaca tattatggag aagattcgcg attaaatata
     3841 ggtccaaagg gatttactgg tgaaaagtat ggaggagcta cttattggga tacagaagcg
     3901 ttttgtgttc cagtatatct aggtgtagca gataaggagg taactagaaa tcttttagaa
     3961 tataggtaca atcagttaga acaggcaaag gataatgcaa aaaaactagg gcttaaaggt
     4021 gctttatatc caatggtaac atttgatgga gtagaatgtc ataatgaatg ggagataaca
     4081 tttgaggaaa tccaccgaaa tggaagtata gtatatgcaa tatataacta tacaaattat
     4141 acaggtgact ataaatatat aaaagaaaaa ggaatagatg taataataga aatagctaga
     4201 ttttgggcaa gtagagttca tttgtctaca aaaaaagatt tgtatatgat acatggagta
     4261 acaggtccaa acgagtatga aaacaatatc aataataatt ggtatactaa ttatatagct
     4321 aaatggtgtt tagattatgc tgtagaaaat atcttacgat tagagaaaga gtgtaatgaa
     4381 tgtattgaca gaaataatgt gactagggaa gaagttgagt tttggaaaat aatatcaaat
     4441 aaaatgtatc tgccgtatga tgaggaacta caaataataa ttcaaaatga taatttttta
     4501 gataaagaat ttgtagaagt aaaggattta ccaaaagaaa attttcctct taatcaaaat
     4561 tggtcttggg ataagattct taggtcgtgt tttataaaac aagcagatgt attacaggga
     4621 atttactatt ttggaaatag atttactaaa gaagaaaaaa agagaaattt tgacttctat
     4681 gaaaaatata ctgtccatga atcttcttta tcttctagta tttattctat tattgcctca
     4741 gaaatagata acctagaaaa ggcttatgaa ttatattcaa gaacagctag gcttgattta
     4801 gataattaca ataatgatac aaatgatgga ctgcacataa cttcaatgtc tggttcatgg
     4861 ctttccattg tacatggatt tgcaggcatg agaacatgga atgaaactct atcttttgac
     4921 cctaagttgc caaaagagtg gaaaaattat tctttcaata taaattatag agataatatt
     4981 attaatgtag atgtatgtag tgatttaatt aaaatagaaa atttaaaagg taaaccaata
     5041 aaa
//
LOCUS       DACXMZ010000246         5032 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.247, whole genome shotgun sequence.
ACCESSION   DACXMZ010000246 DACXMZ010000000
VERSION     DACXMZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.247"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            351..581
                     /locus_tag="JD016_17145"
     CDS             351..581
                     /locus_tag="JD016_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HAU5001342.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            594..743
                     /locus_tag="JD016_17150"
     CDS             594..743
                     /locus_tag="JD016_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HAU5001343.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            1000..1437
                     /locus_tag="JD016_17155"
     CDS             1000..1437
                     /locus_tag="JD016_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HAU5001344.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            1694..2587
                     /locus_tag="JD016_17160"
     CDS             1694..2587
                     /locus_tag="JD016_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAU5001345.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            2765..3589
                     /locus_tag="JD016_17165"
     CDS             2765..3589
                     /locus_tag="JD016_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAU5001346.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            3715..4296
                     /locus_tag="JD016_17170"
     CDS             3715..4296
                     /locus_tag="JD016_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAU5001347.1"
                     /translation="MKADSTKEQLINATIQLLSSHRDVSSITAREIVAKANVNLAMIN
                     YYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFDFIGEHSQFI
                     KISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSFIQLLFLRSN
                     AFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            4400..4576
                     /locus_tag="JD016_17175"
     CDS             4400..4576
                     /locus_tag="JD016_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001348.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            4731..5032
                     /locus_tag="JD016_17180"
                     /pseudo
     CDS             4731..5032
                     /locus_tag="JD016_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429518.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1949 a          592 c          666 g         1825 t
ORIGIN      
        1 actaatacta acaagcaaaa cactaatatt gacaattgaa atactaatac taacaagcaa
       61 gacactaata ttgacaattg aaatattaat actaacaagc aaaacactaa tattgacaat
      121 tgaaacactg atactaacaa gcaaaacatt aatattgaca attgaaatat taatactaac
      181 aagcaagaca ctaatattga caattgaaat attaatacta acaagcaaaa cattaatatt
      241 gacaattgaa atattaatac taacaagcaa aacattaata ttgacaatta aaataccaat
      301 attaacaagc aaacaattaa aatataaatt attatatcgg aggtaattat atggaaatta
      361 agaaaaaatt agtcatgagc tttaaaaatg taaaggataa acaagtttct ttttctatag
      421 aaaatccaaa ggatgattta actgaagaaa atataaaatc tgtcatggat ttgatagtat
      481 ctaaaaatat tttttcggtt ggaggatttg acctagcttc tgttgttgaa gccaaggttg
      541 tggaaacaaa taccactcct tatgacttag ttatagaata gggggctaat tttatggatg
      601 atagtttaca actgttaata accaatgttg gttttcctat agctctatcc atgtacttat
      661 tggttagaat tgagggtaaa ttaaatactt tatcaaacag tattgataca ttatccaaaa
      721 atattttgat atttaagaag taatttgaca atatatgtaa gtcataaaat aggtctattt
      781 gtaaaataat tagacatatt ctatgtgaaa tactaggata tttggataat ttatccaaat
      841 atccatagta tttttttgaa ttttaccacc tcaaatttga atttcactgt tttatttttg
      901 aatttcacta tgcttaattt gaaatttaga acgcatctag ttggactcaa taagatataa
      961 tgctattcta atgacagtaa taaaatgagg tgatgattaa tgaaaataca tgtcgaacaa
     1021 ggtattaatt gtgatgatat tgaaataacc ataaaatgtt ctgctctaga taataacatt
     1081 gaaaaaataa tttcattatt gagtacaccg ttacttgata taaaaggtaa aaaatcagga
     1141 gagatttata aactcaatgt ctgtgatatt ttatactttg agacagttgg aaataaaaca
     1201 tttgcttatt atgaagatga tgtatttgaa attgatttaa aaatatataa attatcaaaa
     1261 ttattagcta atacatcctt ctcacgaata tctaaatcca tgattttaaa tatggactac
     1321 ataaaaagta taaaagtaat atcacataat cgcatgcaag ctactttaaa aaacaacgaa
     1381 aaagttatta taaatcgtcg ttatataaaa aatattaaag aaaagctaaa catttaataa
     1441 aaatctaaag taatttatta atatcactaa ttgcaaattt attgacctat ttaataatta
     1501 ataataatat aatatattca atattaaata ctaattaatt atatttagtg taaaaataat
     1561 taatcaaaat ataaaaaata ggatattatt ataggcaata actaacatca agcgataact
     1621 taatacatat aaaaataaat aaacaattaa taaaaattaa aacttcatta taaaacggaa
     1681 ggatagtgaa aaaatgcaac tatataacac cccctttaat acaaacaaac acttaatcta
     1741 tttctttaca tttacgctag tattgaactg gatattcagt cttacacctg cgttgctcgg
     1801 aattacaaat acaatacttg gagaaatcat tttttatctt gcatatacat caccagctat
     1861 tgtaggtcta ttttttattt ctacaatcta tccacaaagt gcaaaacaag attttattta
     1921 tagataccta agctttaaag aaattagaat aaaatggata ctattaacaa tattattttt
     1981 tgctttaact tttattactt cgttaataat cggaaattac tttaatgtaa aaacttctga
     2041 tatagattgg atacacatag cagtgtttag accttctaaa atagcttata tgctattagc
     2101 aagttttata ttgagtatac ttagtcaaga agctggatgg agaggatatg ccattgacaa
     2161 attattagtt agatttggat ttataggctc ttcaattatt ttagggcttg tttgtggaat
     2221 ctggtatcta gggtcgtatt ttacacctaa tcaaatacca tataatttgg cacaatactc
     2281 cctatttgat gcttttttat ttataccaaa cattgtactt ttaaacttta taattaattt
     2341 tgtatatatc aatacaagta gaagcatttt atcagctggg ttagttcata tgatgtacta
     2401 ttttttcaac attcaattac ttctacacta ccctattaaa ttatgtgtaa tagctcaata
     2461 tgtacaaata atatttggtt taatatttct tgtatatatg atttcttcta ataaatttaa
     2521 acagaaggta aattctgaaa ttgaacagat aaagtcggat gattttgaat ttgagttaga
     2581 ctggtaattt taatatgaat tataaattaa gataatctat gatttgatac gattactact
     2641 taaatataat ttactctcta taatttattg caacatctgg aaaataattg tatttccaca
     2701 aacgcttgaa gtatataaaa acaaaatata tactgattat aagtgaaaag gaggtcatat
     2761 tttcatgttt aaaataggag aggtttcaaa actaacacaa atttctattc gtatgttgcg
     2821 ttattatgat gaacttggta ttttaaaacc tgcaaaaaca gacaaatata caggtcatag
     2881 attatactca gttgaacaga tttcaatatt acaaagaatt gtattattga gagattcaaa
     2941 atttagcgtt gcagaaatag caaatatcgt acataattgg aatgatgaat ttgtaattaa
     3001 ggaattgaat agaaaaaaga atgaaataca aaaggaaata aagcaggaac aacaaagaat
     3061 aaataaaatt gataaattta tagaagctat aaactgtgat aaagatgaaa ttcattacaa
     3121 tgttgtcttt aaaaagattc caagttataa aatcatatca cttagagaga taattccaga
     3181 ctaccaatct gaaggtatac tctgggaaaa gctatctaaa tttatcaaag aagaacatat
     3241 agaagtatca cgtcaatcca acaataacat agctttttac catgatgaag aagtcaaaga
     3301 taatggtgta gatattgaag ttggaatggt tgtaaagaag attggaaaga acaaatctgg
     3361 atttatatat cgtgagacag aagaaattga tatgatggca tgtactatgg tttatggtcc
     3421 atatgaaaat atagctggtg catatgaatc gttctgttat tggttagata aaaatagtga
     3481 ttatcaaata tcaggtataa atagacaaat cggtcataaa ggtgaacata atgaaataaa
     3541 tcctgaaaat tatcttacag aaattcaaat cccccttata aaagtctaat cgattagtac
     3601 ttaaatactt aagtactaat cacccattga catcccaaaa ttttcatgat agtatctaaa
     3661 cgtgtattaa acatgtttaa tttttttatg gagaaacttt taaggggaaa aattatgaaa
     3721 gcagattcaa caaaggaaca attaattaat gccacaatcc agttactatc ttcacataga
     3781 gatgtttcaa gcattacggc tagagaaatt gtagctaaag caaatgtcaa tttagctatg
     3841 attaattatt attttaaatc taaagaggag ttaattaata tttctattaa aaaattttta
     3901 aagcgtcata tggatgaata taaggctaca catgatacta aaaatttagc acctaaacag
     3961 caattaaggg aaatactaat tgcatttttt gattttatag gtgaacatag tcaatttata
     4021 aagatttctg taccctatat attgatacag gaagaaattg tttatccatt tgagatacta
     4081 cctttaatta aggctcacta tgaaaatcag aataacaaaa aaactgaagc tgaatacaga
     4141 actattgcgt atcaaataac ttcatttatt cagttgcttt ttcttcgttc taatgctttt
     4201 ttcaagtata gtggtattga tattcttaat gttgaacaga gaaatggttt aatagatttt
     4261 caattagatt tattattggt agatattaca aaataatact atattaattt tttatgtaca
     4321 tttttttatt ttatggtaac gtttgaaaat attctcattg actaaaaata cttgcattaa
     4381 ataaggggga ggaattttaa tgttttttac atttatagga gtaatatcag caattttatt
     4441 gttgtgtaca attatatgtg gtttatggat aaaacttaat aatcgtaata atgactctaa
     4501 ttctcacttt actttttcaa tactttcaat tgctttttca ctgttaattg tggtggcctt
     4561 tttagtaaga aattaatgtc tttgttatgc tggggtttat tattatagat ttttattatt
     4621 gcatgatttt tactatatta tagtattttt attatattac agggtcttat ttattatagt
     4681 gttttgttgt tacagcattt tattatatta cagagtccta tttattatag gatttttatt
     4741 atattgtaaa atcttattta ttataggatt ttgttatatt acagactttt attatattgc
     4801 aatctcttat ttattgcagt gttttgttat tacagtattt ctattatatt gcaaactctt
     4861 atttattatg gtgttttgtt attgcaacat ttctattata ttgtaaagtc ttatttatta
     4921 cagttcttta ttatactaca gacttttatt attacaacct cttatttatt acagttcttt
     4981 gctatactac agacctttat tattacagca tttctattac attgcaaagt ct
//
LOCUS       DACXMZ010000247         5007 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.248, whole genome shotgun sequence.
ACCESSION   DACXMZ010000247 DACXMZ010000000
VERSION     DACXMZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.248"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1767
                     /locus_tag="JD016_17185"
     CDS             <1..1767
                     /locus_tag="JD016_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001349.1"
                     /translation="NGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSISLL
                     VSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIETM
                     MLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVVLS
                     QFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYKEI
                     MVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQGI
                     LKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSIEH
                     IKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTLLK
                     ILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVYKS
                     NLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDADM
                     YIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKLLE
                     SGRHDELMDKKGVYFNLFCNCK"
     gene            1789..3210
                     /gene="ccpM"
                     /locus_tag="JD016_17190"
     CDS             1789..3210
                     /gene="ccpM"
                     /locus_tag="JD016_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HAU5001350.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVNKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSENSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            3239..4309
                     /locus_tag="JD016_17195"
     CDS             3239..4309
                     /locus_tag="JD016_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HAU5001351.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            4325..4576
                     /locus_tag="JD016_17200"
     CDS             4325..4576
                     /locus_tag="JD016_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HAU5001352.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            4627..4830
                     /locus_tag="JD016_17205"
     CDS             4627..4830
                     /locus_tag="JD016_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001353.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
BASE COUNT         2038 a          451 c          803 g         1715 t
ORIGIN      
        1 aatggtaaat ttcaattttt taaaggtgta agaaattgtt ttaatatgat ttatctacat
       61 gataaaagat atttatttat ctctatgttt atggcaattt ttcaggggat tgttccatct
      121 atttctttgt tagtaagtca aaatatcatt aataatatgc aaacattttc taaaagtgta
      181 aaagaaatat tagttttgtt aattatgtat ttaatcataa atataattcc ttcaattttt
      241 caaagttttt atggatatta tagaataaag tttaataaaa attttgaaaa acatattgaa
      301 acaatgatgt tagagaaatc tttaactttg agtttaaaag attttgagga tgataatact
      361 tatgacatga taaatagagc tcaatctcaa aaaggattta atattactat actatctgaa
      421 aaccttattt caataataag agaatcagta tctacacttt ctcttattgt tgtattatca
      481 caatttcaac tttggctaat aatagttgtt attgttattc ctgtattgaa atcattatat
      541 tccattaaat taggaaagga acaatatgat gttagtgtaa acagaacttg gaaagaaaga
      601 aaggcctggt acatagatta tttgttgtca aaaggttatg cttacaaaga gattatggta
      661 tttagaattg gttctaaact tattaatcat tataaaaaat taaaagagga tattatatct
      721 caagatatga atatagctaa aaagaatttt attgctacgc tattatgtac agttttagat
      781 tcaattgtat ctgcaggaat tataggattt ataataattc aaggtatttt aaagaagatt
      841 ttaataggag atgcgactac atatatacaa gcagttcaat ctataaagga taatgttgaa
      901 tcaatatttt cgttatttag ttcagtaata caagatcttt tttatacaga tttactgttt
      961 aaatttctag attataactg taaagaagac tcaattgaac atataaaaat atttgaaaaa
     1021 ataggggata taaataaaat agagttaata aatatttcat ataaatacaa tcattcatca
     1081 gagtattctt taaaaaatat taatttaact ttaaataaaa atgatcctat tgctatacta
     1141 ggaaaaaatg gttcaggtaa aacgacactt ttaaaaatat tattaggttt ttatgatgat
     1201 tatgaaggtg acatcttgat aaatggaaaa aatttaaagg atattgaaaa agatgaatac
     1261 attagaaaag taagttgtat gtttcaagat tatattaaat atgaggctac attaaaagaa
     1321 aatgtcgcca tgggagatgt atataagagt aacttggaag aaaaagttaa aagtaaactt
     1381 aaagaaacta aattgaaacc aagtatttat gaggaagatg gattagaaac tattttagga
     1441 acctggtttg gtaagaagca actttctatg ggtgaatggc aaaaagtagc aatatctaga
     1501 actcttatga aagatgctga tatgtacatt tttgatgaac cagattcatc tttagattct
     1561 gaagcgggaa atgaactcct ttctttatat aaaaaaacat ttgaaaataa gattggaata
     1621 tttataacgc accatatcca acatgcaaaa aagataacta acaatattgt agttcttgaa
     1681 aatggaaagt tgttagaaag tggaagacat gatgaactta tggacaaaaa gggtgtttat
     1741 tttaatttat tttgtaactg caaataaata ttaaagggag gttaattaat gaatcaaaaa
     1801 ccatttatac acacatttaa agctggaaaa tcatatttta tttatgatgt taatacggat
     1861 aagattttaa aggtaaatgc tgctgtttat aattatctaa ataaaattga gcataattta
     1921 gaaaaagaaa gtgactggaa catagaaata gaagacgaaa ttaatacctt gattaattat
     1981 ggatttctaa aaaataaaag ggtaaataaa acaatgcacc ctgaaactaa gtatataaaa
     2041 tatcatatag aaaataatct atcctctatt attttacaag taacccaaaa ttgtaattta
     2101 cgttgtgact attgcattta ttctggttca tacagcaaca gagttcatac aaataaaaga
     2161 atgaatatag atgtagcaaa aagaagtatt gactatttga ttaagaattc tagtcaatta
     2221 aatgaagtta gtgttggctt ttatggagga gaacccttac ttgagtttga tttgattaag
     2281 cagatagtag aatatgtaaa agtagttgga gaaggaaagg aaatatactt taatctaact
     2341 acaaatgcaa ctttatttaa tgaggaaata atagaattct ttgtaaaaaa taatataaca
     2401 actatgatta gtttagatgg aggaaaagaa attcatgata aaagtagaaa atttgcaaat
     2461 acaggagaag gatctcatga tgttgtatta aaaaatgtac gttacataaa aaataaatat
     2521 ccagaatatt ataaaaagaa tattacattt aatacagtga taaattcaga aaatgattac
     2581 atttctatag atgattttat atctaaaaat gagactttaa aagattcaaa ctttttagct
     2641 tccactctta ataataacta ttctgataaa gaaatagttt catcagatga atatatatta
     2701 ggtaagagtt atgatagatt cttgacttta ctcaacaaat taaaatttat aaaagatgag
     2761 agtatgtcac ctttatcaag aatagaaaga gccttattag atgattttag atatgagaaa
     2821 aatattgaag gtagattaga gttaccagat gtgtggcatc atggtggacc atgtatacca
     2881 ggagctttta gaatatttgt aactacagat ggagaatttt ttccatgtga gagagtaagt
     2941 gaaaattcta aaatgggtat gattggaaat ttagatagtg gagtagatat agaaaaggct
     3001 gaaaaacttt taaatatagg aaaattatat gaagagcaat gttctgattg ttgggcctat
     3061 agttattgtg atatatgtat agctaagatt gatgatacta aatgtaatga aaatgaaata
     3121 aaaccacatg attgtgacag aattagacat tctacagaaa ataatatgaa agattactgc
     3181 gttttgaaat ctctaggata tcaatattaa attttctaat gaaaggaaag atgggattat
     3241 ggaaaaagta cttatatatc catttaatta taattttttt actattttaa atcattgcaa
     3301 taaaatttca gatatagaaa ttaattcact ggttagtcct tctggctggg ggttatgcaa
     3361 taaggagtat aatgttaata atattattac taaagttaca aatgattttc aaagtgaact
     3421 taaaaagaat tctttggtat ggtttgttga ttcaaaagaa aatctagact tagaaaaatt
     3481 tataattcct aaattaaaag tagctctaaa agaaggtaaa aagatattat atacacgtga
     3541 tgatcttaat aaaatatcta ggtatatatc tcattatgaa ttcaaaaaga ttaatgaatg
     3601 gaatttagaa aaatatgaaa taagagatat agagggtata tcacagataa acacaccaat
     3661 aatatttatt tgtggtcttt atggagggtt agatactttt gatgtaaata taaggatagg
     3721 agaagaacta agaaaaaggg ggttttcaat attacaattt ggccctaaaa aagagagcta
     3781 tttatttaaa atgattccta taccagagtt tatgtttgat gattcgataa gtagtaagac
     3841 aaagattatt aattttaacc attttataaa agataaagag gtagaggcaa agccagattt
     3901 aataattata gaagttccag gagaattatt ccctttctct agaaaaatgg ttggtaattt
     3961 tggaattaga gcttttgaaa tttctaatgc agtaataaat gattgtggta tattatgtat
     4021 tccatcaagt atatatgata gagaatattg tgaagaactt aaatatttta ttaaaggaag
     4081 acatggtata aatatagact attttagtat agagaaaaaa gttatagata catatgagac
     4141 agaggtgaag caaaaatttt catatatgtc attagatgaa aacttttttg ttgaagatat
     4201 agaagattat gaaaaaacat tttttataca tgataataaa caattactag aattagtgga
     4261 caatatagtg aatcaattaa aagagtatgc taaagtaata tctatttaga attggaggtt
     4321 aattatgaat tacactgaaa aggaattact actagagaat atattttttg atgtgactgg
     4381 aattaatttt aataaaaata acaatttaaa aaaagaatta tttttttcac cgtctttaca
     4441 tatacagcca agagaattac ttttagtatt ttgcgaggta atagaaaact ttaatataaa
     4501 aattccagag gaaaaacttt tgaataggca atttacgagt tttgataatg ttctaaaaat
     4561 aatcaatatg gtataggtta ataaaggtat aaagtaagtg atcttataga aaaggggagg
     4621 atatgaatga gttttggaaa aagaatagaa atgaataata taggtgttga gacaagaggt
     4681 gtttgtacta ttatgtgtcc ttatacttgt aagatgatgg catcttcatc ttgtaactgc
     4741 aagaataatg attataatgc aagaggagga tcatataata catattataa taaattgata
     4801 catggccaaa gaactcctga tggaatgtaa atatttcagt aataagtata ctattaaatt
     4861 gatttagcat atttgattaa atttatcttt cttactttat ttccttttat tataaaaata
     4921 tgcagttgat tataattaag agtgtgattt tataataact gcataaatag tatctgttat
     4981 ataaatcacc tcttcaatta ttatagt
//
LOCUS       DACXMZ010000248         4977 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.249, whole genome shotgun sequence.
ACCESSION   DACXMZ010000248 DACXMZ010000000
VERSION     DACXMZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.249"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(128..1318)
                     /locus_tag="JD016_17210"
     CDS             complement(128..1318)
                     /locus_tag="JD016_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAU5001354.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(1578..2009)
                     /locus_tag="JD016_17215"
     CDS             complement(1578..2009)
                     /locus_tag="JD016_17215"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAU5001355.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            2122..4350
                     /locus_tag="JD016_17220"
     CDS             2122..4350
                     /locus_tag="JD016_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAU5001356.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVNVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(4461..4946)
                     /locus_tag="JD016_17225"
     CDS             complement(4461..4946)
                     /locus_tag="JD016_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAU5001357.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
BASE COUNT         1769 a          773 c          671 g         1764 t
ORIGIN      
        1 aataaaaagc aggtatcaat caaatagtaa catgtattat actatacatt actatcaata
       61 ttgtacctgc tttcatatta taagcaataa agaaaaatct atttattagg ttttataata
      121 aaaacaatta taaaactctt cttgcattta catatctact tgaatagtag ctactattta
      181 tattagatat tgttactttt tttgatgaac ctgaagatgc atgtatcata tctccatttc
      241 ctatgtatat tccaacatga cttacagaac cattatttga accttgtgta tcaaagaata
      301 ctaaatctcc acttctaagg tctcctctat ttacatatgt tccatactta ctttggtctc
      361 tagatactct tggtatacta actcctacag attttttcat aacgtactgt gtgaatccag
      421 aacagtcaaa actatttgga ccttctgctc cccatacata tggttttcca agtagtgtct
      481 ttgcaaaact tattacacta tcagctcctt gacttggaga attagtcgaa taagatggaa
      541 ttgaagtatc aacagaacct ttttcagcaa ggtattgact tgacacatat ccagtttgat
      601 tgccataact tacttttgtc catcctccac tttcagaaat acttccaacc tcagtaccat
      661 aacttaatgt agctatttta gaatgacttg tacctggtcc tgttctaacg ttcaatgact
      721 ttgctgttac aacctttgta ccctttacaa ggtcatttga actgttttca gcattttcat
      781 ttgtagagtc atttacacta gttcctaaat atttacttga aacataacct actctaccat
      841 tagaacttat ttttgaccat ccatttgact cagatatgta tccgacttca gtcccatatc
      901 ctagtgttga tattttagaa ctacttgtac ttggtcctgt tctaaaattt aatccttttg
      961 ctgttacaac ttttgtcgac ttaactgaag tatcactact ttcattgcta cttccaaggc
     1021 tatttattgc tacataattt ccatgcacat aacctgtgtt tccattatat ttaactttta
     1081 cccaagaacc ttcttttcct acgtactcaa ctttatcacc tttgtaaagt ttccccattg
     1141 attcatggtt tgtacctggt ccttttctaa aatttaccgc attccctgtt attgttctgc
     1201 tttctatact cacactctct acttcagact cattagcata tgctaaattc cccatagtag
     1261 gcattattat cgcagttgcc attatactgg ctgctattct cttttgatta aaattcataa
     1321 ctttccactc atctcctctt ttctactatc tttatttcac ttcaaaatat aaaagaaata
     1381 attgacacta ttaaaatatc aattatttct tttatattaa tattattaca aatttgtaac
     1441 tatttgtcta tagtaatatt tgcttaccat aaattaccat gtataaattt taatctttaa
     1501 tagcctgtaa tttagtaata tagccatctt ttaccttttt taaaaatggc tatatttttt
     1561 aacttttttg taacttttta aaatccagtt gagccaaacc caccttttcc tctttcggag
     1621 tcacttaatt cttttacttc ttctatgtta atatcataga taggttttac tatcatttga
     1681 gcaattttca tatgcttttc aaccttaaaa tcatttttac catgatttat taatataatt
     1741 ttaagctctc ctctgtatcc ttcgtcaata gttcctggtg tatttaatac agtaacagaa
     1801 tgctttaagg caagcccact tctaggtctt acctgcgctt ctgtcatagt aggaagctct
     1861 atacatatcc cagttttgat aagtttagtc tctccaggag gtattacaac ctcttctata
     1921 gaatacaaat ccataccagc atcacctttg tgagcaaaat taggtattat agcatcatca
     1981 tttaattttt ttacttttaa attgtacatc ttatatctcc tttcaacttt ctataaatat
     2041 tataacttaa atttcgacct cttgaagttt tttatttttt tgtggtaata taattattag
     2101 tgttttaaag gaggattttt tatgaaactt gtacttgctg agaaaccatc tgtggcaaaa
     2161 actatagcat catttttagg tgcaaagacc agacaagatg gttactttga aggaaatgat
     2221 tatataatta cttatgcagt tggacactta gtatcattat atgatatgaa ggattatgat
     2281 aaagataaat actctggctc ttggaaaatg aacaatttcc catttgttcc tgaggataag
     2341 tttaaattta agattgatag ttctaaaact aaacaattca acactgtaaa aaagcttttg
     2401 aatagaaaag atgtagaata tgtaataaat gccactgaca atgaccgaga aggagaactt
     2461 atagcatttt taatattttt actggcaaaa aataaaaaac cagtaaaaag aatcttggta
     2521 aatgaatgga caccagaaga tattacaaga ggtataaaaa acctaaaaga tgaagatgaa
     2581 atgagaaatc ttcaagctgc tggatatact aggcttatta ctgactggtt gattggaata
     2641 aatttcactt ctgttgctac attgaaatat ggaaatggga aactattaaa cataggtagg
     2701 gtaattcttc caacagttaa attagtctat gatagagata tggaaatact aaactttgtt
     2761 cctaaaactt attatgaaat agaagggcat tttaaagctg aagctggtga atataaaggt
     2821 aaatatgtta agggtaaaga aagtaaattt gatacacttg aagatgcaaa taaaattata
     2881 gcttcaatta catctgaaac tggtaaaata ctagataaaa aagttactat gtcaaaggaa
     2941 tatgcaccaa aactttttag tttgacatca cttcaaggat atataacttc taagtattcc
     3001 aattttacat ctgataaggt tttaaatgtc tgtcaatctc tctatgaggg taatggtaag
     3061 ggtggataca taacttaccc tagaactgat tccattttct tagaagaaag tttggcttca
     3121 aaagcttctc aaactttaga taaattaaag gttggtcttg aatatgaaaa taaaattaaa
     3181 ttttctaaaa ctaaaagagt ttttgattct tctaaagtag actcccatag tgctataatt
     3241 ccaacttata taattccaaa aagtttatct aaggatgaac aattagttta tgatgccata
     3301 aaagatagat ttgtagcaaa ctttatgcct cctgctgaat atgagaatac agaaattaag
     3361 actgaagtag ataattgtac tttcttaaca aaaggtaagg tgcttaaatc aaaaggatac
     3421 cttgaggtat ataataagga agaaaaaaac gatttacttc cacttgtaaa taaagatgat
     3481 gttgtaaatg tgttagaaat aaaaccatta actaagcaaa ctactccacc taaaccgtat
     3541 actgaggaca cacttttaaa ggctatgaaa aattgtggta aaaatgtgcc tgaagaagat
     3601 acaacagtgc tatctggata ttcgattgga acatcagcca cacgtgctga tgtgttaaag
     3661 aagataagtc aagtgggcta tgtaaagaaa aaaggtaaat cttattctat aacagacctt
     3721 gggaaaaatt tagttgaaat ttttcctgtt aaagacttat ttgatgtaga ttatactgga
     3781 aaacttgaaa aaagtttaag cgatattcaa aaaggaaagt atacaagaaa agaatatctt
     3841 actaatatca ttagttttat atatcaaaat gtcaatatta taaaacaaga tgcttcaaaa
     3901 aatataaaca cagaagcata tactttcgat tcaaaaacta aaaaatttac taaaacaaaa
     3961 gaaaaaaaac agaaaaaaga tgtaactaat actgaaaaag aagttgctat atcaaaggat
     4021 tctaataaca aaaaagaagt taaaaatggt gatgaaaatc aatctcttgg caaatgcccc
     4081 atttgtcaag gtgatgtact agaatttgac aaaggctttg cttgtaaaaa tcataaagaa
     4141 tgtaaatttg ttatttggaa aaatgataag tatttaagtc tttataaaaa gaaagttaac
     4201 aaaacaatgg tcaaaaatat tttaaaaaaa ggtgaaacta aagtaaaatc tttaactgct
     4261 aaaaacggta ataaatttga tgctacatta aaatatgata aaaatccaga tactggctat
     4321 ttcaattgga aaatagagtt tgacaactaa atattcataa gaaaagaaat agcagtagta
     4381 taataatcta tattatatta ctgctatttc ttttcttatg aagttttaac ttttttaata
     4441 gtgactttta caatcacact ctaacctaat ttctttacta tttctgataa ctttaatgta
     4501 tgttcatcta ataaagttag ttcctcttca gaaagattag ataatttttt tactagagat
     4561 tctttgagaa tagcttttac tgcatcaaat aatttaaatg ctttatctgt taattcaacc
     4621 ctaagaatcc ttctatcttt tgggtcagga aatttattga ctaaaccatg ttccactaat
     4681 ctgtcaataa tcggagtcat atttgatttt aaaattccta aattagaagc tatttgagaa
     4741 attgatgatg aatttgatgt cataagataa agaattacct tagcatgtga tggagccatt
     4801 ggacattctt caaaataagc tttcattata tcagagttat tttgaaactt ttttaacaaa
     4861 tcgctttctt taaatatatt attttcaagc acaaataaaa aatctaaaaa agagtccgct
     4921 attttaagta tattgttgtt atccatattt gtaacatccc ttcaatagat aatataa
//
LOCUS       DACXMZ010000249         4972 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.250, whole genome shotgun sequence.
ACCESSION   DACXMZ010000249 DACXMZ010000000
VERSION     DACXMZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.250"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            46..1113
                     /locus_tag="JD016_17230"
     CDS             46..1113
                     /locus_tag="JD016_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HAU5001358.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            1132..2292
                     /locus_tag="JD016_17235"
     CDS             1132..2292
                     /locus_tag="JD016_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HAU5001359.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            2305..4401
                     /locus_tag="JD016_17240"
     CDS             2305..4401
                     /locus_tag="JD016_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HAU5001360.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(4560..4904)
                     /locus_tag="JD016_17245"
     CDS             complement(4560..4904)
                     /locus_tag="JD016_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAU5001361.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
BASE COUNT         1891 a          576 c          920 g         1585 t
ORIGIN      
        1 aattttaaaa attgaattat aacttcaaaa aataaggagg gtaatatgaa gataacagat
       61 atcaaatttg aaaagcttag aattaaatta aaaaaacctg tagtagtttc atttggagta
      121 attgaatatg gagaaagtgt aattttaaaa attgaaactg atgaagggta ttgtggattt
      181 ggcgaagcag cacctcttgc agcagttaca ggtgaagttt tggataatgt tctttcagta
      241 ttacttatgt ttaaaaaaga gttaataggc aaagacccac tagatattga aaccatacat
      301 accatcatgg atggaattat tataggtaat acttctgcaa aagcagctat tgacatagct
      361 ttatatgata ttaaaggaaa aattatgaat gcaccccttt ataaagttct tggaggattt
      421 gatagtaaag tccaaacgga tattactata agtatagata agccagaaaa aatggcagta
      481 gaagctttag aaagagtaaa agaaggtttt aggatattga agcttaaagc aggtataaat
      541 cctgaagacg atattgaagc tgtaaagtta attagagaag ccgttggaga gagtataaga
      601 attagaattg atgcaaatca aggatggaac gtaaatagca gtataaatac tatcaaaaag
      661 ttagaagaat ttaatgttga tgcaattgaa caagcacttc cacattggga tttagatggt
      721 actgcttaca taagaaataa aagtaacaca aaaattatga tagatgaatc attacattca
      781 ccaatagatg caattaaggc tataaaaaag aatgcagttg atacatttaa tattaagctg
      841 atgaaatcaa gtggaatata tcctgctata aaaatcaaca acatagctga ggcaagtgga
      901 gtaaattgta tgttaggatg tatgctggaa actagaattg gtataacagc agcagcaaat
      961 ttaatagcat ctaaaaagaa cataacagaa gctgaccttg atagttttat gttttgtgaa
     1021 gaattagaag gtatatctgg aggatttgta atggatggag atattatgaa tcttgtcaat
     1081 aaaccaggtt taggaataga agtaaactta tgataaattt ggaggttaaa tatgaataca
     1141 cttttttcaa atccaggtag tatacttgct gtaatgacat caatgattgc attgggtttt
     1201 tacttacaaa gatacaaagc cattaaaagc ttaggaccag cacttacaat aatcataatg
     1261 ggaataatac tttcaaactt aaaagtagtt ccagtaagta ctgagttata tggaactatt
     1321 tcaacatatg ctattccagt ttctatgact ataatgttga tgagtgttga cttaaaagaa
     1381 atgacaaaat tatcaagaga acctttaatt gcaatttttg tagctgtatt aactgtaagt
     1441 atcatggcat ttttatttgg attagtattt gcagaaaaaa tatcagaagg ttggaaggtt
     1501 gctggaatgt ttgtaggtac atatactggt ggaagtgcta atcttacagc tattggaaca
     1561 ggtcttaatg taagtagaca aactcttgct gcagcaaatg cagcagacta tgtaattgga
     1621 gtaccaacat tgatatttat gtttgctcta ccagcaatat taaaaaattc aaagaagttt
     1681 aaaaaacttt ggccatatca tgtagaagaa tcagaattag aggattgtca aaatgaagaa
     1741 tttatggagt caaaagaatg gagtattaaa gatatagctt ggatgttggc tataggtttt
     1801 gttgttacag aagtttcaac aatacttgct ggatatttca attcaagttt tagtagtgca
     1861 gcaagaatac ttttagttac aacaatttca attataattg ctcaattaaa gccagtaaag
     1921 aaactaaagg gaaatttaga tttaggatta tttgttgcat tattcttttt atgtacaata
     1981 ggtttttcag tagatataaa agagttttta ggttcaactt ttactataac actttactgt
     2041 tttagcataa tttttgcttc atttgttttc catttaggca taactagact tttaaagata
     2101 aagtatcaat atgtaatact ttctatagta ggagcaattg ctgatggtcc tacttcagct
     2161 ttagtagctg cttctgcaaa gtggaattcg cttgtaagtg tagcagtcgt aatgggagta
     2221 attggtggag tattaggaaa ttatgcaggt atatcagtgg catatgcaat aaagatgtct
     2281 ctaggattat aggggggagt ttaattgata aaatttaaat tatacatatc tggactatac
     2341 agtggaaatg taatctttga tggagattta ttaattgaaa agttgaatcc ttttacaaat
     2401 aaaatagaaa gtttaaaacc aataagcaaa gaagaaaata catactattt aaatcttaca
     2461 aaaatagatt taaagtcttt atttaataac tttgatgtat atactaaaag cttagtaaac
     2521 acagataaag acacagttat aaataactta ggagaaacgc attctaaact taatgaatat
     2581 atatgggttc aaagaaacaa aaagttccca ctagatataa ttattgtaga taataaaata
     2641 gtaggtttta tctgtttatc cagagaaact tgtactatat taattatgga tggatatgaa
     2701 gagtatactg tattaaaaga atgggaaaaa actcataaaa atgaagagat atattctata
     2761 agatttggtg gaaattatat gattgatatg aaagatggta taaaactttc tacagatgta
     2821 tatctccctg attttgtaga ttctaccaaa aaagcaccaa caattcttat gagaactcca
     2881 tatggaaaag aaaatgataa agaaatatat tataaatatg tacaaagagg atatgctgtt
     2941 gtaatacaag atgttagggg aagaaatgaa tcagaaggta agtgggaacc tatgattcat
     3001 gagagggaag atggagatag taccataaat tggatagtat cacaggaatg gtcaagtgga
     3061 atagttggaa tgcttggagc atcatatctt ggttatgtcc aatgggcagc tgcatcttca
     3121 ggcaataagc atcttaaagc tttagttagt atagtaacat caggaagccc ttttattgat
     3181 ataccaagaa aaggaggagc ttttgtatct ggtatgcttg catgggcatt tatggtatca
     3241 agaaataaag ttgatagaag caaaatggta agagatgatt gggatgatgt tttaaacata
     3301 cgaccaatag aaaatatacc tgttgaagct cttggatata gaattgaatt tctggaagaa
     3361 tggttaaaga gagtagaaaa agatgagtat tgggatttga tggattggca tttacaaaaa
     3421 gataaaataa atgtacctgc attggttgta tctgggtggt atgatgataa tagtatggga
     3481 actacagagg ccttagatgt tataaaggac tatgaaaaag gaaagagaaa ggctattcta
     3541 ggtccatgga tgcataattc aaatacttta agagatataa atggtatttc tttaggaaat
     3601 agttcattaa gatatgattt ggattataat tatctattat ggtttgataa atacttaaaa
     3661 ggtatagaaa ataacataga tacgactgct ccagtagagt attactctgt aggttctaat
     3721 aagtggaaga cagaagaaaa ttggccaata ataaataaga tagataaaag tatgtatcta
     3781 ataagtgatg gaaatgctaa cacatcttta ggaaatggta gattagtatt tgataatgat
     3841 ttagaagaaa agtatgatag ttatatatat aatccaaaag acccatcagt acaactaatt
     3901 gatatgtcag agaatgaagt tggagttcca aataactaca aagatttaga aaaaagaagt
     3961 gatatgttgt gttatacatc agatgctttt agtgaagaat ttactgtaac aggtgatata
     4021 aagttagagt tttttgcatc aagttctgcc aaagatactg actgggttat aaaaattatg
     4081 gacgtggatt tagatggaaa ttcaattaaa ttagctgatg gtatacttag tgctagattt
     4141 agaaatagtt tttataagtc agagtttatg gaagaaggag agatttataa gtttacgatt
     4201 ataacttcaa aaatatcaaa cacgtttaaa gttggtcata aaattagact agatataacg
     4261 tctagtgcta aaaactttat tttccaaaat agcaacacga cagagggata taatagtatt
     4321 gaatatatag aagcaaagaa tacgatttat catggaggta aatatccatc taaattgata
     4381 ttgccaatag agaataagta aatttatata gattaaatag tgaggaggtg tctcaaaatg
     4441 aattttttag ttcgtgagac actttctttt atacaaaatc aaatgtattt tcatcatttc
     4501 aacaatgaaa aagaggctta tctctctgtt ttgagatagc ctcctataag ctaaattgat
     4561 tattttgtag ctgcaacaac gctcatttca actaacaagt gctctctagc aagtctagct
     4621 tcaacacaag ctctagcagg ttcaaatcct ttttcaaccc aagcatccca aacagagttc
     4681 atagcttcaa agtctttcat atctcttaaa taaatagtaa cagatagcaa atgttgctta
     4741 tctgaaccat atttgtttaa taaatcttct attttagcta aaacttcagt tgtttgttca
     4801 attactcctc cttcagcatg agtttgtcca cataaataaa ctgtattgtt atgaactaca
     4861 gctctactca ttcttccagt tccttcatat ctctttattt ccatatttta aatacctcca
     4921 tagttaaaaa atctattaaa aatctaaata ataaattaaa aaaagtctat ac
//
LOCUS       DACXMZ010000250         4899 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.251, whole genome shotgun sequence.
ACCESSION   DACXMZ010000250 DACXMZ010000000
VERSION     DACXMZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.251"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(73..1425)
                     /locus_tag="JD016_17250"
     CDS             complement(73..1425)
                     /locus_tag="JD016_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAU5001362.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            complement(1624..2952)
                     /gene="ssnA"
                     /locus_tag="JD016_17255"
     CDS             complement(1624..2952)
                     /gene="ssnA"
                     /locus_tag="JD016_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HAU5001363.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            complement(2980..4563)
                     /locus_tag="JD016_17260"
     CDS             complement(2980..4563)
                     /locus_tag="JD016_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HAU5001364.1"
                     /translation="MRILIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            complement(4579..>4899)
                     /locus_tag="JD016_17265"
     CDS             complement(4579..>4899)
                     /locus_tag="JD016_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAU5001365.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKS
                     VYKK"
BASE COUNT         1417 a          995 c          581 g         1906 t
ORIGIN      
        1 ttaatatctc tgaattattt ttattaatac ttaatgactt taagtcaata tagattttta
       61 cttgttttat ttttattttg cttcttcaac tttattattt ttttcttttt tagaacctag
      121 ttcattgaag aatatgttta aaagcactgc tgataaacta cctgttgtta ttccactatg
      181 gaatattgtt tgtacccaag ttgggaaatt atgatagaaa tcaggatttg ttagtggtat
      241 catagacaaa ccaacactaa ctgctactat aaccccattt ttagttccat caaatttcac
      301 tttgcttaaa gtttttactc ctccaaccat taccattcca aacattgcaa atcctacgcc
      361 tccaagaaca ggcttaggta ttgctcctat tattgctgca aattttggta agaatccaag
      421 tactagcaat ataacccctg cgtaaactgc tataaatctt gattttatac ctgtaagacc
      481 tactattcct gtattttgac caaatggagt tattggaaat gaattgaata ttccagataa
      541 tgctgtagat attccatctc caaatagacc tcttttaata tttttacctg ttacttcttt
      601 gtctaccatt tcatgaatag ctatcatatt accaactgat tctgtcataa gtaccaacat
      661 aatcaaacac aatgatagta ttgcagatgg ttcaaatcta ggcattccaa attttaaagg
      721 tacatttaag ttaagccaac caactccatt tactgcacta aaatctgcca ttccaactgc
      781 catggaaagt agtgtaccaa gtactatacc taccaatata gctgtgctat tccaaattcc
      841 ttttaagaat ttaaatgaaa gtaaagttat tgccaatact gctaaagcta atgctaatga
      901 gtttggttca accgcttcta atttgttgtc agttatccat tttatcccaa ctggaaatag
      961 tgaaatacct attaccataa caactgttcc agtaactaca ctagggaaaa atcttattaa
     1021 tttttcaaat actggagcaa gtaatataca aaacaatcca gctactatta cagagccaaa
     1081 taccgtctgt attgcaactt gtgaatcact atatgatgag gctattgctg tcatacctgc
     1141 cacagcggca aaactagttc cttctattac tggtatttta gcacctacaa tatttcctat
     1201 accaacactt tgtatgagtg ttcctatacc tgccataaac aagcaagcat ttattaaaaa
     1261 aacaatacta gcatgatcta gtcgtgcagc acttcctatc atcataggaa ctgctactgc
     1321 tcctgcacac attgctaaaa cgtgctgtag tgaaaatacc caagatttgg atacaggtaa
     1381 cctttcatct actggagcta ttttagggct tttatctcga gacattttgg gtctcccttc
     1441 tatatttaat ttaatattct attccgtata ttttttattt atatacttca tttaaattcc
     1501 tatttttgtt taagctctgt ttttaacatc taaattcttg tttcttactt gtatattaat
     1561 ctgaacctcc catgactaaa gtcacagggt tcctgcttca tagaattttg cataatcaat
     1621 tatttaaatt ctattccata gattttgact taattctcta ctttttgcta gaatactttc
     1681 ttcatctatg tttattaact ttctatcttt cataagtacc tttccattta taatagttgt
     1741 atctacgctt cttccactca ttccaaaaat agtgtgtccc cctattgtat tttctgtcaa
     1801 tggagtatgt ggtatataat caactactat aacatctgca taagcacctt ttttaagtac
     1861 tcctaggtca tttttataat aagctttagc tatttttcta ttgttatcaa ataacattgt
     1921 ttgtgattct tcaaatccaa ctcttgggtc ttttaagtga tgtttgtgaa ttatattggc
     1981 aacttttaat gattcaaaca tatcgctagt atatccatca gttcctaatc ctactgtaac
     2041 tccccttttt aataactcta ttgcaggaga acatccaact gcatttccca tattagattc
     2101 tggattatga actacaaatg tatttgtttt atttaaaaga ctgatttctt ctgcacttat
     2161 atatatacaa tgaactgcta ttgttttttc ttttagtact ccaaaatcat tgaatctttc
     2221 taaaaccctt tttccatatt tatctacatt atattgtaag tctgctagtc cttcagcaac
     2281 atgtacatga taacctgtat caagaccagc catctctgaa gcacatttat ttaatgtatc
     2341 atcagatagt gtaaatgcag catgaagccc aaacattcct tttatcatgt ctgtatcatc
     2401 tttttgagcg tattttataa agtttacgtt ttcattgata ccttgttgga ttgtatttcc
     2461 accatctcta tcagacactt cataacaaag acacgttcta attcccaaat cctttgcaac
     2521 atctgatatt gtaaacaaac tgccttcaat tgcatttgga cttgcatgat ggtcataaac
     2581 tgttgttaca ccatttttta tacattctat atacgttgca tatgcactgt attttgtatc
     2641 ttccagtgta agctgtttgt caagtctcca ccataaattg tttagtatct ctaaaaagtt
     2701 ctcagaaggt tttcctggaa ctgccattcc tctagcaaat gagctgtata tatgcatatg
     2761 tgaatttatt agacccggca tgattatttt cttgcctgca tctataaatt catgtactgg
     2821 atatttagct ttcatttctt ctgtagtacc tatatcttca attagattat catttattac
     2881 tacacatcca tcttccatat agggatttaa agaatcttgg cttattagcc tcccattccc
     2941 cactaaaatc ataaatttat tcctccttaa agctaagatt taatttttaa gtacttttcc
     3001 tgctttaatt ttattgtatt ttccttcttc aacaacacaa tttccattta taattacgta
     3061 atctatacct gtagggtact gcatagagtt gataaaatca tcacaaccac taactgtatc
     3121 cttgtcaaat ataactatat ctgcaaaata tccttctttt aattcgcctc tatcttttat
     3181 actaaagctt gtagctgcct ttttagtcat tttataaaca gcttcttcga ttgttaacac
     3241 attcaactct ctaacatatc ttcctagtat tttagggaaa gaaccataag ctcttggatg
     3301 tggtttacct gctaaaagac catctgtaca cacattttgc tcgtctcttt tcatgaatcc
     3361 tattatatgt tcttctaatc catagaaatc taccatccca actgcatttt cttcttcttt
     3421 taataaatca aacgttgcat ctaatgggtc ttttcctctc atttttccaa tttctaataa
     3481 gctcttacca attgtatctt tattcttttc tgtttttacg cttgttacaa atatttggtc
     3541 tattccagca aattctataa aattatccca gccttctatt ccatttgcta tatctttttt
     3601 catttttgct ctttcgtttt catcacttaa tctttctatt aacttatcag ttcctccaga
     3661 atgtgcccaa ggaggtaaaa ctactccaag cattgtactt cctgctgcat atggatattg
     3721 gtcaaaagat actcttattc cttccttttc tgctttttct aaaagctcta taacttgtgg
     3781 tatatacttc cagtttgctt taccacaaac cttaaaatgt gaaaaatgaa cttttactcc
     3841 tgattgtttt ccaatttcta tgatttcttc cattgaagtt aaaattgtat ctgcttcact
     3901 tctttggtga actacaaata ctccgtcata ttttgctact accttacaca tctctataat
     3961 ttcttcagtc aatgagtatg cacaaggtat atatataagt ccagtagaca atcctatagc
     4021 tcctgctttt aattctctct ctgtgatttc acacattttt tgaatttctt ctttagtagc
     4081 aggtctatct tcaagtccca tagcttccat tctaacgttt ccatgaggta ctaaataagt
     4141 ttcatttaaa cctaccccat tgtaatccat catttttaaa taattctctg ttgtttcata
     4201 tttccaatca atttcatcac tttcaccatc tagacctgct aaattttttc tccaagaaga
     4261 tatatgctct tgaggtagag gggccataga tataccatct tggcccaaca cttctgttgt
     4321 aattccctgt ctaatcttta cctcattata tggattaact aaaataacta ggtctgaatg
     4381 gctatgagta tctatgaaac ctggacatat aactctacct tttgcatcta taactttgtc
     4441 agcttcttcg tttaaatctt gtgatatttt taatattttc tcgttttcta ttaaaatatc
     4501 tccttcatat gctggtgttt tatttccatc tactataagc ccatgtttta ttaaaattct
     4561 cacaaaacca tctcctaatt attttttata aactgatttt aatataccat aatatccttc
     4621 tgcacctttg aataattggt ctaattctat atactcatct attgtatgag caagattttc
     4681 cttagaagga ccaaatccta tagttcttat tccagcctca cctgcgtagt gacttccatt
     4741 cgtacagaat gagtattgag ttatttctga atctattcca gcttctttta atcccttata
     4801 tgctgcttga acaaattcat cttcttcgtc atataaccaa cctgggaaga atctttcact
     4861 ttctatagta tttcctgtat agcaatcagc tttttcaac
//
LOCUS       DACXMZ010000251         4776 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.252, whole genome shotgun sequence.
ACCESSION   DACXMZ010000251 DACXMZ010000000
VERSION     DACXMZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.252"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(406..1047)
                     /locus_tag="JD016_17270"
     CDS             complement(406..1047)
                     /locus_tag="JD016_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAU5001366.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            complement(1290..2093)
                     /locus_tag="JD016_17275"
     CDS             complement(1290..2093)
                     /locus_tag="JD016_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAU5001367.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDVHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            complement(2249..3424)
                     /gene="thiI"
                     /locus_tag="JD016_17280"
     CDS             complement(2249..3424)
                     /gene="thiI"
                     /locus_tag="JD016_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAU5001368.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEESTILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            complement(3470..4621)
                     /locus_tag="JD016_17285"
     CDS             complement(3470..4621)
                     /locus_tag="JD016_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAU5001369.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
BASE COUNT         1480 a          925 c          455 g         1916 t
ORIGIN      
        1 tattataaaa aaaaagaacc cttttataac taagggttac tttttttctc tattttttct
       61 tattatatca ctattttttc cctatttcaa tattttgaca ttttttccat tatactgaaa
      121 agatatctta aatttttaac ttggataaat tttaaagcat atacttaaaa ttaacatact
      181 tgttttctat tttattacac ttaatcctta tttttatcat tctacaaaac aaaaataaaa
      241 aagaataaga taataaataa aatacttatc accttattct ttatttaatt attttacaac
      301 aaaaaatcta aactactttt taaatagctt cctcttccat caatttattt gtatattact
      361 gatttaattc ttcaagtaac tgattggttc ttgcaatact ttatcctaaa ctctggaaac
      421 tttataaact acctctctag catttggtaa atttccatct ggaataccta cagcaatacc
      481 aaatggtaca gaataccctt ctggtatatc aagtagttct ctgtattttt tatttgtatc
      541 aaacttcatc tttattgact ctgattcttt tacatcttct ttatccaata cactcacaag
      601 accttcaata tccccttgtt caacaggtgt aaataaacct ctaaccattc ctattataca
      661 acttccaagc cctagtgctt gtgcagctgt tgttatattc cctgtaagta ttcctatatc
      721 atatggagcc catgaactac tttcatcata agaaataaat aaaacggttg gagcaccata
      781 aaataactta aagtttttat ttatctcaac attactcacc tcatgaatcc tagataaagt
      841 atcatcacta atctctttta aaagtttctg gtcttgcaca acagttatgt gccaaggctg
      901 tttattacat cctgaaggtg ctgcaaatgc acagtgtaat aatgtgttta cttgctcatc
      961 actaatttgt tctgtcgtaa attttcgtat acttctacga ctttgtatta aatttattgt
     1021 ttggttgtct tgaaaattat tattcatatc tattcccctt tttatgtatt tcttagtaca
     1081 aatactaaat cttcagaaaa ttaatcttat aattaaattt ctttacaata ttattataat
     1141 tatttttagc ttgtatgtga agacttaaaa atataaacac ttttaatata tctcaattat
     1201 atatataaat gaaaatctgg tattcgtcat ctatagaaac tgctcttttc tatagtttaa
     1261 tcaataccag attaataaaa catgatatat taatttaaag gaccccatcc ctctttagaa
     1321 tgaatctctg tttttacaga cttaacacta tctgtgtcaa catcccatga cctacctgca
     1381 acagaaatac catttttacc tttataccat ctaccttcat cataatcgta aactagggaa
     1441 gtataccaat tacccccagt cttattattt gcaaatacaa ctacagtagc atcagattta
     1501 gtaggattat atgcatatgg ttgtatatat agtgtatcct tacttaatcc atattttgcc
     1561 ttaaattcat ctggaattgt aggccaccca tttttatatt tattgtctaa taaatatttt
     1621 ctaaaatcat cattgccctt tgaagctcct ggtattatag aagggtcttg tgcaaatgat
     1681 gcaatcaaat ctttcatact ttgatgcaca tccctagcca tttcaacacc gtctggatgt
     1741 tttgtacaag taacatacac ttttgcaatg ctttctattc cagttattcc atcataacca
     1801 tcatcaaacg ttgctgaata tattccacct gacttacact ttggttctgt ttcaaagtac
     1861 ttaccatctt tgttttctaa tacctctttt atgacttcat tcttgtcttt acttgattcc
     1921 tcagccattg caataacaat ttgagtcttt atattttctc tattagaaag acatgtaact
     1981 gctttacttt tttctatatt tctaaataac gctggaacag ctactataac taaaatgcct
     2041 attatagata taactaccaa caattcaatt aatgtaaaac cctttttatt cataataatc
     2101 attcccatta aataaaatta tagtacttat ttttaaatac tacatattaa taaattgtct
     2161 acataaatcc tttcaacaga actactaata tacttacaga actactaata tactcaatat
     2221 tcaatttagt aattaaataa cttgtatgct aaaactcaat atcctctacc tctattttgt
     2281 caatagcatc ctgtactaat ttttcaatgt caagtgctgt ttcagatttt tctattttct
     2341 ctaatctagg tttagtgacg ggctttctag gagaaaatac actacaacaa tcttcttcag
     2401 gcactataga tgtttcaaaa gtccctattt ttttggctat atcaactata tctgatttat
     2461 ccattgctat aagtggtcta aacactggta agtctacaac ggcattagta caagtaagtc
     2521 cttgtatagt ttgagatgca acctgcccaa tactttcacc tgtaattaaa gcatcacaat
     2581 gtcttttttc agaaagtctc tgcgctatcc tcatcataaa tcttcttgaa agaatagtag
     2641 attcttcttc attacaattc tcgccaattg ctttttgtat ttctaagata ttaactttat
     2701 gaagccttac tctaccacag tattttgcca gtattttagc taaatcctta actttttcct
     2761 gtgacctttc acttgtaaat ggataactat gaaagtgaac tgcctctact tccatacctc
     2821 tttttgcaac catccatgta gctactgggc tatctatacc tcctgataaa agagacatag
     2881 ctctaccatt cgttccaagt ggcaatccac catatccagg tatagtatca ctatagacca
     2941 tagtatgaaa ttctctgtat tcgcacttta ttttaacttc tgggtttctt acatcaacat
     3001 taatcctatc tcctaccttt gataataagt atccacctat atccatactc atttcttgag
     3061 aagtcaaccc gaaagactta tctcctcttc ttgactctac cttaaaagtt ttatatccag
     3121 cctctatttt ttcttctaac atttttaaag ctatttcttt taatgtatca taatcttttt
     3181 tagctcttac tccagggcac acacccacta tcccaaatac ttttctaact tcttcaatta
     3241 cttcttcata atcatagtct tctaaatcaa catatattct tccatactct ttatatacat
     3301 taaatcttcc aattggtttt aacatatttc ttatatttct tataagccta ttctcaaata
     3361 tatatctatt ctttcctttt actccaattt ctccatactt tactattaat atattataca
     3421 ccatttcacc tcttaaacta atttattatc actatttata cagtattctt tattttcttt
     3481 tcattattga ccttaagtca caaattgatt ctttcaaaac tttcacagct tctaacattt
     3541 cttcttctgt attcatatca gataaactaa atcttattgt tccatttatt tcttcatttg
     3601 taagacctat agcatttaat acgtaactac ctttcttttt agaagaacat gcagagccag
     3661 ttgatacata aatacctttt tgctctaagt aatgaagtaa aacttctcct tttgtccctc
     3721 taaatgatac atttaatata tgacatactc catcttctgg agaattgatt tttatatctt
     3781 ctatattatc aattatttca ttttttaata tattttttaa gttatctact ttcgaaataa
     3841 cagcatctaa atctttgttt agtattctaa cagcttctcc aagaccatat attccaggta
     3901 cattttctgt tcccgaccta attccaattt cctgaccacc acctgttaac ataggcttaa
     3961 ggcgattatt ttcttttata tacataaatc caattccctt tggaccatga aatttatgtg
     4021 cacttacact cataaaatct atattgtatt tagatggtct aaagttaatt tttccatatg
     4081 actgaatagc atcaacatgg aaataaattt tttcttttaa acccttcaaa tactttccaa
     4141 cttcacctat tggctgtata gttcctattt cattgttaac atgcatcatg cttactaagc
     4201 atgtagttga ctttatcgca ttttttaaat cctctatatt tattttccca tcttttccga
     4261 cctctaaata agttacttca aaaccatctt cttctaaatc ttttaatgta tttaaaacag
     4321 aaggatgttc tatgttagta gatattatgt gattttttct ttttttattt aaactagtaa
     4381 tacctcttat tatagtatta ttacactctg taccacctga tgtaaaataa atctcctttt
     4441 ctttcgctcc tagagattta gctatatctt gtcttacttc ttttatagct ttttcaactt
     4501 ctacaccttt tctatggaca gaagaaggat ttccatattc cgtattaagt gcataaacca
     4561 ttttatctat aacttcttga taaggttttg tagttgcgct attgtctaag tatatttcca
     4621 acttaattac acctaccttt atttaaaatc ttcaactcta actattatac cactaattat
     4681 agtatttttt ttgttaatct ttattaaaaa gacaatctta aattataaaa tattatttca
     4741 tgaaaagcta gttttttcac tttattacaa attttt
//
LOCUS       DACXMZ010000252         4758 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.253, whole genome shotgun sequence.
ACCESSION   DACXMZ010000252 DACXMZ010000000
VERSION     DACXMZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.253"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     misc_binding    306..543
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            607..2526
                     /gene="thrS"
                     /locus_tag="JD016_17290"
     CDS             607..2526
                     /gene="thrS"
                     /locus_tag="JD016_17290"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAU5001370.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3294..4385
                     /locus_tag="JD016_17295"
     CDS             3294..4385
                     /locus_tag="JD016_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAU5001371.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT         1880 a          475 c          852 g         1551 t
ORIGIN      
        1 atattaagta ttgattgtat ttaagtatac aaaacctaaa aagagtacaa aaattagaag
       61 ttagttttaa taaaaattag ttttagtatt tatctagtca tatctatcat gcatgtggat
      121 acgttttaaa aattttaaat ttatgaaaat attgtaatat cactataaat caagagatta
      181 atttaatata aaaatataag aatattggtt tacagctgat tgagatgaaa aataatgggt
      241 aattaatata ttacattaat gttgattgat gtataactat ctgttataat gaaaatatat
      301 taaaaatcat agtgttgaag aggaagagta gagtagaaac cattctcaga gaatttgggt
      361 ctatggctga gagcccatgt aataggtcta tttgaaaact accttggagc tgagcttatt
      421 agcagtattt gttttgtact atacgtgaaa gtgctcgggt gacgacctta tagtcatcaa
      481 gagggttgta tatacttgta tacgccaact tgggtggaac cgcgaattaa tatcgtccca
      541 aacatatttg gggcgatttt ttattacaaa aaatctaaaa attatttcaa taaggaggaa
      601 aaaacaatga ttaaagttgc tttaaaagat ggctcaataa aagaatttga aaatgctatt
      661 tctgttatgg atgtagctaa atccattagt gaaggattag ctagaaatgt ggtagcagcg
      721 tctgtgaacg gagaggttgt aggtctggac cacattatag atactgattg tgacttgaat
      781 ttatttaagt ttgaagataa agaaggtaag gaagttttta gacatacatc agcgcatata
      841 ttagctcaag ctataaaaag actttaccca gaagcaaagc ttgctatagg accaagtata
      901 gaaaatggat tctactatga tatagattta gaccatagat tagttccaga ggacctagag
      961 aaaatagaag ctgaaatgaa aaaaatagct aaagaagatt taaaaataga aagatttgaa
     1021 cttcctagaa atgaagcttt agagcttatg aaagaacaag gagaagacta taaggtagaa
     1081 cttatatctg atttacctga aagtgaaata atatctttct acaagcaagg tgattttact
     1141 gatttatgta gaggaccaca tttaccatct actaaaaagg taaaagctgt taaattacaa
     1201 agtgtagcag gtgcatattg gcgtggtgac gaaaataata agatgcttca aagaatatat
     1261 ggaacttctt ttgagaaaaa caaagactta gaagagtact tacatttact agaagaagct
     1321 aaaaagagag accacagaaa attaggtaaa gaattaggat tatttatgat acctgaagaa
     1381 ggtccaggat tccctatgtt tttaccaaaa ggtatggaac ttaaaaatga attattaaaa
     1441 ttctggagag aaatacacag aaaagctgga tatattgaga tagaatctcc aatcatatta
     1501 aatagaaaat tatgggagac ttcaggacac tggtatcatt acaaagaaaa tatgtataca
     1561 gttaaaatag atgatgagga ttatgctata aaacctatga actgtcctgg tggattaatt
     1621 tactataact ctcaactaca ttcatataga gatttcccaa tgagagttgc agagcttggt
     1681 agagttcata gacatgagtt atctggtgca ttacaaggat taatgagagt tagagcattt
     1741 actcaagacg attcacatat attcatgtta ccagagcaaa ttaaggacga aataaaagga
     1801 gtagctaact taatagatgg tatatacaaa acttttggat ttgaatataa tttagaatta
     1861 tctacaagac cagagaactc tatgggaagc gacgaagagt gggaagcagc agaaaatgga
     1921 ttaagagaag ctttagaaga gttaggtcta ccatatacta ttaatgaggg tgatggagca
     1981 ttctatggtc ctaagataga cttccatcta aaagattgtt taggaagaac ttggcaatgt
     2041 ggtactatcc aattagatat gcaacttcca agacaatttg ataatactta tattggtcaa
     2101 gatggtgaaa agcatagacc agttatgatt catagggttg cttttggtag tatagaaaga
     2161 tttataggta tattaataga gcattatgct ggtaaattcc cagtttggtt atctccaact
     2221 caagttaaga tacttcctat atctgataaa tttatggatt atgcaaatga agttaagaaa
     2281 gagttatttg ataagggtat aagagttgaa ttagatgata gagctgaaaa aattggattt
     2341 aagataagag aagctcagct tgaaaaagtg ccatacatgc taatagtagg tgaaaaagaa
     2401 gtagctgata ataatgtttc tgttagatca agagataaag gtgaaatagg tagtattaaa
     2461 ttagatgaat ttatagctag tatttcaaaa gaaattgaat caagagaaag tattattcaa
     2521 gactaatata tattatatta gtagctaaga tattgttata taagataagt ttaatcaata
     2581 tgttgatatg ttgatgcata gtgattaaat ttatgaaaag atgagtataa ttagaggtac
     2641 agttaatcat atttttatga ttaatgtacc tctagttttt tgtatggtag atttattaat
     2701 aaatatatca tagaagtgtt ttgaacatgg aaatatttat gtgcattctt taaataaaag
     2761 tttatttttt gagtgataat taagtgcttg ttggtaaggt tatggctgta aaaatcataa
     2821 ctttattttc atatttgttc cattttcata aaaattatta tccctttaag aaaatttctt
     2881 aatcctcttg aaatatttcc tttttagtct aaagttaaat ttctttcttc aaaatgaata
     2941 tttaaatgaa ctaaaattta agtacatttt ctaatcttat gagtctattt ttcatagata
     3001 attgccctat aatagaagaa tctatgttgg agctatgtgg tgacaataac agcattgtca
     3061 gtgattaatt aatgctttat aaatgattaa ttagtgtttt atgttatttt tggacttacc
     3121 ctctatacat agattaattt atttaacttt agatatagag agaatgtcaa gtacatacac
     3181 agacattaga gtttaagaaa gtgtttatgg agcaagaact tttaaaagag gtagtgactt
     3241 agtttaattt caagaaatga taaatacaaa agaggaaaaa gagaggtgtt gaaatgatag
     3301 aaaaagtaga agaaaaatct aagattatta aaacatcaaa agaatataag cgaactataa
     3361 ttgaagaaaa agataaagaa tggaccaaaa aattaaatga tatacttagt agaagtagtt
     3421 tttctaatga tgaattagat aaaatcaagg cgttaattcc aaaagagatt ttgactaatg
     3481 aaaatgttgg agaagttgtg actgaagatg gatatgctaa gcctgagtat gatgaggtat
     3541 ttaaatcatt gttcacttta aataatgaat atgatttact tgtaaatttt ataaatgata
     3601 tcctaaaaga tgctccatat gctaacagga atatcaaacc gttcactcaa ataaagagaa
     3661 taattagagt tgaaactgac ccaacaatta attacattgg agagaaaaga cctagacttg
     3721 atattttggc tgaagatgaa gaaaataatc atataaatat tgagatgcaa agagcttttg
     3781 agaacgatta tttggaaaga gctgaatatt atcttagtcg tgtacatggt agaaagttag
     3841 aagaaggtaa agagtataaa gaaattggaa aaactattgg aatacatata ctaaatcatg
     3901 taaaatataa tcacatagaa gattatgtaa attgcctgag attaacaatg gacgggcatc
     3961 cagatattta ttctagtaaa actgcattat attttataga gcttccaaaa attcataaat
     4021 ctgactatat agtaaataga attatgctat ggggaaaact tattagtaat ccttctaact
     4081 tagatgtaag agttatagct aagaatgatt ctattattaa aaaagcactt gatagattaa
     4141 aagaattagg tagcaatgaa gaatatttat taaatctgaa aagaggtgct tatatcatga
     4201 ataaaagtag aaattttaaa gaagagattg aaagagagac agcaattaga gttgcaaaag
     4261 aaaaagatta taaaataatt ataatgttga aaaaaaatgg ttttaaatta gatgacatat
     4321 taaataaatt taatgatttt gacttaagtg aagatgagat aaaagaagta tatgaaaata
     4381 attgatgata aataaaaagt gaacttatat aagtttgctg aatcggagtc tggaaatatt
     4441 tatgtgtatt ttttaatgaa tgaaaattta ttttaaaaac aataatttag aagtacagtt
     4501 aattatattt ttatgatact gtacttctag ttttttctat tgtagattta ttatttacta
     4561 ataaatctac aatagaaatg tttttgttgt aagaatcatt ttatctaatg attgtttttt
     4621 ataatagtta aatttccatt tgtagtgttt aaatataaat ctataacagc atttctataa
     4681 atgttataaa tcactataat gattcaactc aaagttactg cttaaagaag cagagatttt
     4741 atcaaggtat atcatgga
//
LOCUS       DACXMZ010000253         4719 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.254, whole genome shotgun sequence.
ACCESSION   DACXMZ010000253 DACXMZ010000000
VERSION     DACXMZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.254"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     misc_binding    45..276
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            384..1865
                     /locus_tag="JD016_17300"
     CDS             384..1865
                     /locus_tag="JD016_17300"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAU5001372.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            1875..2768
                     /locus_tag="JD016_17305"
     CDS             1875..2768
                     /locus_tag="JD016_17305"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAU5001373.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            2895..3953
                     /locus_tag="JD016_17310"
     CDS             2895..3953
                     /locus_tag="JD016_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HAU5001374.1"
                     /translation="MIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVIS
                     GVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEAV
                     KAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIVV
                     EGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSSS
                     DVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAIR
                     NKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAYK
                     IDKLSTVEDVINELISEL"
     gene            complement(4108..4620)
                     /locus_tag="JD016_17315"
     CDS             complement(4108..4620)
                     /locus_tag="JD016_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAU5001375.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
BASE COUNT         1753 a          588 c          754 g         1624 t
ORIGIN      
        1 ttgtctgttt tataaactag aataattaat aatattaaga atattgaaag gcattgatta
       61 ggaagagtaa atctgaatcg attaccagag aaggaaacta ctagctgaaa tgtttcccta
      121 attagaagga ttgaaaacta cctaggagct tctatccgaa gttatcttaa tgattttagt
      181 aggaatagac gtttttatta cttgcgttaa agtatcaagt ggatttttta aaatcaattt
      241 gggtggaacc gcgggaaaca gattctcgtc ccttttttag ggatgagggt ctttttttat
      301 tacctaaatt cagtttttaa aatattctgt atctaattta attagctaat taaaaattta
      361 aaatatatgg aggattataa aaaatgtatt attcaagtac gagaggcacc gaagaaaaag
      421 taactgcatc acaagcaata ataaaaggaa tatcaagtga tggtggtctt tatgttccaa
      481 gtgaatttcc caatgtaaaa aatgaactta taaatttagt aaatttaacg tattctcaga
      541 tagcattttt tgtacttagt aaatttttat gtgattttac tgaagatgaa attaaaaatt
      601 gtatagaaaa tgcatatgat gaaaagtttg attgtagtag tatagctcca ttaaataaag
      661 taaatgatac ttatttctta gaactatatc acggtccaac tttagcattt aaagatatgg
      721 cacttactat aatgcctcat cttcttaaaa catctataaa gaaagataat ttagagaaag
      781 atgttgtgat tcttactgct acatctggag atacaggcaa agctgcactt gagggattta
      841 aagatataga taaaataaag ataattgtat tcttcccaga agatggtgta agcccagtac
      901 aaaaacttca aatgaaaact caaactggaa aaaatacata tgtagttggt ataaaaggaa
      961 attttgatga tgcacaatca ggtgtaaaga agattttttc tgataaagaa tttaatcaaa
     1021 aactattgga taataattat atattatctt ctgcaaattc aattaatata ggaagacttc
     1081 ttccacaagt tgcatactat ttttattcat atatgaaatt agttagcaat agtgagataa
     1141 atttaaatga taaaataaat tttgttgtcc caactggtaa ttttggtaat attctagctg
     1201 gatattatgc caaacaaatg ggtcttccaa taaacaaatt gatatgtgca tcaaatgata
     1261 ataatgttct ttatgatttc tttaaaactg gtgtatatga taaaaacaga acattgaaat
     1321 taactacttc tccttctatg gatatattaa tttcaagtaa tttagaacga ttactgtttg
     1381 aaatatcaaa tagaaatact gatattgtaa ataagctttt atcagacctt agtgataaag
     1441 gagtctacaa aataagtgat gatatggaaa aaaatttagc ctcattctat ggagaatatt
     1501 caaatgaaga agaagttaaa agcacgataa acaatgtgtt taaaaattat aattatttga
     1561 tagatactca tactgctgtt gcatacaatt gttatgaaaa atataaaaaa gaaacttctg
     1621 ataacacaaa aactgttatt gtaagtactg caagtccatt caagttttct gaagatgttc
     1681 taaagtctat agattgtgat tttaaagacc ttgatgactt tgctataatt gatagattat
     1741 caagtatttc taaaatagat attccaaaac ctattaaaaa tcttaaaaat gcagaaatac
     1801 tacataaaga tatttatgaa aaagatgaat taaaactagc tataaaaaaa ttcttaaagg
     1861 tataggtgat tattatgtta gagattatag ttcctgcaac aagtgctaat ataggtccag
     1921 gatttgattg tcttggaatt gcgttaaata tatacaataa attttatgtt gaagaaattg
     1981 aaagtggtct tgaaatagaa ggttgtgaag atgcttacaa aaacgaaaac aacttagttt
     2041 atacatctat gaagtacttt tttgatagag ttaaaccaga aaaaatacct gctggtatca
     2101 aaataaaaat ccaaagtgaa gtacctatat gtagaggtct tggcagtagt gcctcttgta
     2161 tagtagctgg agttatagct gcaaatgctc tatcaggtgc taatttagat aaaaatcaat
     2221 tactaaatat agcttcagaa atagaaggtc acccagataa tgttgctcct gctattttag
     2281 gaaatatgat tgtatctgtt actgataatg agaacataca ttatgatatt ataaaaattc
     2341 ctgaagaact taaattttgt gcaatgatac ctaattttaa gttatcaaca gaaaaagcta
     2401 gaggtgtttt accaaaggaa ataccatatt ctgatggggt attcaatgtt agtagagttg
     2461 ctttactaat atctgcctta ctaaacaaaa actttgacct acttaaagta gcttgtcaag
     2521 acaaattgca tcaagattat agaggaactc taattgagaa ctacaatgat attgttgaaa
     2581 aatctgaaca attaaatagt attggagtat ttttaagtgg agctggacct actataatgt
     2641 ctttaataaa agaaaatgat gatagttttg tggataatat gaaaaattac ctacaaaaac
     2701 taaaatctga ttgggaaata aaagagttat gttgcgattc taatggagct gttttaaata
     2761 taatataatt atcgtcttct atacaaaata taactactct tttattataa tttgtagtat
     2821 aataattagg actaggtaat aatattaagt attataatct agttctttaa acatgtttac
     2881 attgaggagg gattatcata aaagagttaa taattagaga tttagttgca aaagttccta
     2941 taattcaagg tggaatgggc gttggtatat ctaggtcatc actagctggt gcagttgcta
     3001 agcttggtgg tgttggtgta atttctggag ttcaaattgg atatgatgag gaagattttg
     3061 aaactaatac tataaatgca aatttaagag ctataaaaaa acatatttct aaggccaaag
     3121 aaatctcaaa tggtggtatt attggtatta attttatggt agctatgaaa gaatatgaaa
     3181 catacgtaaa ggaagctgtt aaagctggtg tagaccttat aatatctgga gctggattac
     3241 ctaataagct accatcatta gtaaagggaa gtaatgtaaa aatagcaccc atagtttcaa
     3301 ctgcaaaagc tgctaatgta attttaaaaa tgtgggatag aaaagagaaa actactgctg
     3361 acttaatagt tgttgaaggt ccaaaagctg gtggtcacct tggatactct aacgaggaac
     3421 ttgataacat agattctata gactatgata aggaatttgt tgaaatttta aaggttgcaa
     3481 atacttatgg agaaaaattt ggtagaaaca taccagtagt tgctgctggt ggtataactt
     3541 ctagtagtga tgttaaaaaa tatatagata tgggtgcaag tggtgtacaa gttggtacaa
     3601 gatttgttgc tacttacgaa tgtgatgccc atgagaactt taaaatggca tacataaatg
     3661 ctagtgaaga agatgttcat atagttaaaa gtcctgtagg tcttcctgga cgcgccataa
     3721 gaaacaaatt tatagaagaa gttaaaataa atcgtccaga aataaagaag tgctataatt
     3781 gtttgattcc ttgtaatcca aaagaaactc cttactgtat ttctcaagct ctaatcaatg
     3841 cagttaaagg agatgttgaa aattcacttt tattttgtgg aaatgatgcc tataaaattg
     3901 ataaattaag tactgttgaa gatgttatca atgaattgat ttctgaatta taatttaaag
     3961 aaaactaaat taagatttta tcaagctaag gagtttaaat acgttattta ttaatcttta
     4021 taaacatatt tatgtcctta gcttttttgt gtcaatttcc ttttatggaa attttaattt
     4081 aattagaaat agtattaacc tttataacta atcttctttt acaagtacat tggttatttc
     4141 agctatatat aaagtatgat aatctttttg tggataccat cttccatcta taccttcagc
     4201 aataaaacat tgaggttcaa gtttctgact atacattttt ttgcatataa tagccatttt
     4261 agattcttta aaataaggtg tatcatttaa gtgttctata ttaaatccaa tctgtgatat
     4321 cttatcctct tctctaccag atacttttcc acaatatgaa agctctttac gaaaatcttc
     4381 atcaaaaaat gtaagagaaa atttatctgt attatctata aattccttag tataacgctg
     4441 tggtcttatt actacaaaag caacatcttt tccccacata actccaagcc ctccccagct
     4501 agcagtcatt gtattcactt taccttcatt ttctgctgtt ataagtgtcc aatctttacc
     4561 tatcaatcca aatggattat ttttaatctc ttctgccaaa atttctttaa aatcactcat
     4621 atctctaata acctctcatt ctcttatatt tatattcctt ttattttcgt gataaattat
     4681 aaatgaagtc tagttttgaa aataaaatac ttttttaaa
//
LOCUS       DACXMZ010000254         4622 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.255, whole genome shotgun sequence.
ACCESSION   DACXMZ010000254 DACXMZ010000000
VERSION     DACXMZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.255"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..591
                     /locus_tag="JD016_17320"
     CDS             <1..591
                     /locus_tag="JD016_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HAU5001376.1"
                     /translation="ATGVTGATGPTGPTGATGATGATGASAIIPFASGIPLSLTTIAG
                     GLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRDGTITSISAYFSTTA
                     ALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGSISSGIVTGLNI
                     AATAQTRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            760..1521
                     /locus_tag="JD016_17325"
     CDS             760..1521
                     /locus_tag="JD016_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HAU5001377.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            1830..2108
                     /locus_tag="JD016_17330"
     CDS             1830..2108
                     /locus_tag="JD016_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001378.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            complement(2219..2398)
                     /locus_tag="JD016_17335"
     CDS             complement(2219..2398)
                     /locus_tag="JD016_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001379.1"
                     /translation="MQFPFYKITKLIESYEKVEYNQVFFSKQDLLILINFNFKHIKSY
                     YFLLDTLQPALLATV"
     gene            2401..2976
                     /locus_tag="JD016_17340"
     CDS             2401..2976
                     /locus_tag="JD016_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HAU5001380.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            complement(3082..3915)
                     /locus_tag="JD016_17345"
     CDS             complement(3082..3915)
                     /locus_tag="JD016_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HAU5001381.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGTDYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPLGPCHYGIGTLFMQVEYPFCNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(3983..4612)
                     /locus_tag="JD016_17350"
     CDS             complement(3983..4612)
                     /locus_tag="JD016_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HAU5001382.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         1705 a          597 c          745 g         1575 t
ORIGIN      
        1 gcaacaggag ttacaggagc aacaggccca acaggcccaa caggagcaac aggagcaaca
       61 ggagcaacag gtgctagtgc aataatacct tttgcatcag gtataccact atcacttaca
      121 actatagctg gaggattagt aggtacacct ggatttgttg gatttggtag ttcggctcca
      181 ggattaagta tagttggtgg agtaatagac cttacaaacg cagcaggaac attgactaac
      241 tttgcatttt caatgccaag agatggaaca ataacatcta tttcagcata cttcagtaca
      301 acagcagcac tttcacttgt tggttcaaca attacaatta cagcaacact ttaccaatct
      361 actgcaccaa ataactcatt tacagctgta ccaggagcga cagttacact agctccacca
      421 cttacaggta tattatcagt tggttcaatt tctagtggaa ttgtaacagg attaaatata
      481 gcagcaacag cacaaactcg attcttacta gtatttactg caacagcttc aggtctttca
      541 ttagttaata ctgtagcagg atatgcaagt gcaggaattg caataaatta gattagataa
      601 gaaaaggctt ctaatatcgt attgatatta ggagcttttt taagaatgat tgatgaacat
      661 agttttaata ttaattagtg cgcatattat aaaatactat tatataatat aaaataataa
      721 cattaaattg gaaatataga attatctagg ggtgatacta tgaaaaataa gattgaaata
      781 tcaatgaaca actttgtaaa aaattatcat aaatcaaata atataactac aaagtggcaa
      841 gaacttttgt ttgcttatgc atctgctgaa gatgatatgt ttcagaagct aaaaagtgtt
      901 gtaggaacat ctcatgctat gccaaaagat tttctggaaa atggaaaaac tataattaca
      961 tattttatac cttttgatga aagtgtaaat aatagtaata ttggagggtt tgaatgttct
     1021 gaaatatggg caagagcgta catagaaact aatatgttga tttcaaattt aaatgatttc
     1081 ttatgtgaag aactagagaa gataggggct aattcatatg ctattcctgg gtcgtataat
     1141 tttgacagtg aaaaattggt aagtgactgg tcacaaagac atgtagcatt catagcaggt
     1201 cttggaactt ttggtttaaa taatatgctt attacagaaa aagggtgttg tggaagaata
     1261 ggaagcataa tcacaagttt agaattagag ccaactaaaa gaccagaaaa tgaatactgc
     1321 ttgtataaac ataataatag ttgcaaaaaa tgtgtcaata aatgcgtgaa agaagcttta
     1381 aaagaagatg gatttaatag gaataagtgt tatgaaatgt gtatgataaa tgaaaaaaaa
     1441 tacacttata taggtggatg tgatatttgt ggaaagtgta tagttggagt tccttgttca
     1501 gtaataaacc catcaagata atggatttaa gttgactaat aattttaaag atagtttata
     1561 atgaaaaaat tttagttatc aaaataaata taatataaaa actaaataaa tgtgtttaaa
     1621 aaatggataa ataatttgtg ttgattgaca accagttata ctgttgatag acttatatgt
     1681 atcgctgtga tatataagga gggttgagat tttatgtttg atttaatctt tatttttgca
     1741 tagcaagggc tattgcaact atgaaattta atatataaat agccctttgc tattcctaat
     1801 gaaaaattac tatttaatag gagagcaaaa tgagctataa aaaagcaata catatattac
     1861 cagaagaatt acttgaatta atacaggaat atgtagatgg tgaatgtatc tatattccta
     1921 gaaaatctaa taataaaaaa gaatggggaa gtaatacacc tactcgtgaa gagttgtcaa
     1981 ttagaaatat gcagatttac gaagactatc aagttggata tgatttgcaa tatttatcgg
     2041 aaaagtatta tttatctttg aaaagtatcc aaagaattgt attacaagaa aaaagaaaag
     2101 atatctaagg atgagccagt atagttttat aattatactg gctcattttc ttttgataaa
     2161 agtgtttgag gtatagtttc ttttaagtgg atgatataat ttaagtaatt tcaataactt
     2221 atacagtagc taacaaagct ggttgtaaag tatctaataa gaagtagtag ctttttatat
     2281 gtttgaaatt aaaatttatt aaaattagga ggtcttgttt tgaaaaaaat acttgattat
     2341 attccacctt ttcataactt tctattaatt tagtaatctt atagaaagga aattgcattt
     2401 atgaaaaaaa ttacttggga ggtggaatat atctcccagt tacctgtaat taggtattgt
     2461 aaaaaatgtg gaaaaaagac tgaatatatt tgttcaggtt tatttagagt taatgctcaa
     2521 cataaatatt tggatatatg gttaatatac aagtgttcaa attgtgattc aacatggaac
     2581 tcaactatat attctcgtgt taatccaaaa agcttaaatc cagaaatact agaacaattc
     2641 catacaaacg attataatct tgttaagcaa tatgctatga atatggaact tttacataga
     2701 aatggagcag aagttggtat tccagagtat aaaatagtag gacaagaaat taatattaat
     2761 gaccttacac aagttcatat tattagtaaa tatccatgtc agttaaaggt atcaacatta
     2821 ttacgtgaga aactaggttt atctaggaag gtatttgacg aaatgttgga ttgtgggatt
     2881 attagaagta actcaggagt tgatttaaag aaaggtaaat taaattatga aattatgtta
     2941 gatataggca aggagaagca aaagtcttca aattgattag aataactaac ttaatatatt
     3001 ttcaggaaca tcatttaaaa aaatagtgac tttaaaaaat ttaaagccac tattttttaa
     3061 tatgttttat ctatataact attatttttc taggtttttt aatatttcgt aatagtgtgt
     3121 tgtggctatt tcacttgact cgcctttttt acaaaaagat aatttagata gttttggaac
     3181 tacacgttca acgacaattt tattacctat cattttagat tcaagtatct tcaatgcttc
     3241 taaaaatctc ttatcctttt ttgcacgatt ataaaatgat aaaacgtaga cataaaggaa
     3301 taggttataa ttacagaaag gatattcaac ttgcataaat aatgtgccaa ttccatagtg
     3361 acaagggccg agtggttttt taatagtcca atggtcaagt agaaaatcta cagctttgtc
     3421 aagttttgat tctttattta gataattact aaaacggaat gcatttaata tattaagagt
     3481 tggtagtgga tttgagtatt cagtttctgg acctcgacca aaactaaatt tattgcaacg
     3541 ccacccacca tcagtatact gtgtatctaa aaaatgttga aacgtttttt gcattctaat
     3601 gtcagatgca tatcccatat gacagagtac gttagcagca tttgcagtat gacaagggta
     3661 aatacctcca tttggatata gtttgaaacg tccatcttct cgccatgtgc taaatatcaa
     3721 gtcagcacat tctttaagta aagtatcatc agggttcaca cctaactcta atagataaag
     3781 tacactatca agagtagaga aaggagaacc ttttattaaa cgcttatcag gtgtagtcca
     3841 atagtctgta ccattatcat acctgtgtga taatattatt tcaatatctg ataaagattg
     3901 attttctaca ttcatttttt actccatttc atattatatt ctattaaatc tatcaaaaac
     3961 tttattataa tattatttat atttataatt gatttatttt atttaaaatt gcatttatag
     4021 ctgtatcagg aacaactgct aaatcttctg cattgaagta ataaactata ttagaatcaa
     4081 ataatatatt tgaatttgga ggaaattcat catctccaaa ccaaagtata aataccatag
     4141 atgtattacc catatatgta aacctataag ctaaatctcc catgtttact ttttcagctc
     4201 caattttctc cataactaac ttatatttgt ctatttgaga tgtaaaaact tctgaaagcc
     4261 tatatagagt cctgtttaaa aaatttggat aataaacatt gcctcctggt atttctttaa
     4321 aagttacata tttattagtt ctaggaatac ctttagcatt gattaaatat ctaactatca
     4381 agattttaag ctcaaagtta ttgcaaaggc taccattact attccaaact tctccacttg
     4441 gatagtctat aatatattgt ctatcaaaaa aattgagtgt gaatctattt aaatcattat
     4501 tgaaaggtat attacttaaa ttactaattg tatgagggtc taattctttg agtttttttc
     4561 gaacatagtc aaatggaaca ttataattat tttttgttat cttttcatcc ataatttttt
     4621 cc
//
LOCUS       DACXMZ010000255         4605 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.256, whole genome shotgun sequence.
ACCESSION   DACXMZ010000255 DACXMZ010000000
VERSION     DACXMZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.256"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     regulatory      125..229
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            439..2913
                     /locus_tag="JD016_17355"
     CDS             439..2913
                     /locus_tag="JD016_17355"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HAU5001383.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSILRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYIATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            2913..4370
                     /locus_tag="JD016_17360"
     CDS             2913..4370
                     /locus_tag="JD016_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase subunit 2"
                     /protein_id="HAU5001384.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDRYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         1845 a          505 c          868 g         1387 t
ORIGIN      
        1 taaaagagaa ataattgata ataaaaactt aacaaaaaaa ttaatacata aaaaaaaata
       61 aatataatgt gttgacattt aattgaaata ttcatacaat aaaattgaaa ataaatattt
      121 ccaaatgaga ttagcgagag agtcctttat acgaaaaggc gccgaagaag aaatttaaga
      181 aatgccaatt tttagagaag ctttcaggca aaaatatcgc taattgatgg gactctgaag
      241 agacttaagt taagatacta taagtcaccg aagaagcaaa ataatattaa attattaaat
      301 ctttcaggta tacaaacagg gatatatagg ctaggattat tttccatgct gatatatctt
      361 ttttttatga aaaagtacag gaaataatat caaagttact taaatacagt aggtctaatt
      421 ttctagggag ggttatatat gaatgaattg aaaagagtat ctttgtacaa tatacacaaa
      481 gagcttggag caaaacttgt tgaatttgct ggttgggaaa tgcctttaga atatgagggt
      541 ataaataaag agcatgagaa ggtcagaaaa agtgcaggta tatttgatgt atctcatatg
      601 ggagaggtac agataaaggg agcagagtca gaaaaattta tacaaaacct agttacaaat
      661 gatataagcg cattaaagat aaatgatatt atatatacac ctatgtgtta tgaaaatggt
      721 ggagtagttg atgatttatt aatatataaa tttggtgaag aagattattt attagtaata
      781 aatgcaggta atatagataa agatgtagca tggataataa aacaaagtga aggatacaat
      841 gtagatataa aaaatatatc tagtgaagtg agtcaattag caattcaagg acctaaagca
      901 gaagaaattc ttcaaaaaat cacagatata gacttaaact caataaaatt ttataagtca
      961 attccatcaa caaaagtatg tggttgtcct tgtttagttt caagaacagg atatactggg
     1021 gaagatgggt ttgagatata ctgtaaaaat aaatatgtag aaataatttg gaatgaagtt
     1081 ctaaaggttg gaggagaaga tatatgtcca gctggtttag gttgtaggga tactttgaga
     1141 tttgaagcag ctctgcctct atatggacat gaaataaatg aacacatatc tccaattgaa
     1201 ggtggtttat ctatctttgt gaaaacaaat aaagaatcat ttattggcaa atcaatttta
     1261 tcaaaagaaa aagaaagtgg agctaaaaga aagttagtag gatttgaaat gcaaggtaaa
     1321 ggaatgccta gaaatggata tgatataagg attggtgata aaacagttgg atttgtaaca
     1381 acaggatgtg catctccaac gacaggaaaa attttaggta tgggaattat agattctgaa
     1441 tatgcaaaag taggaaatga aataggtata gcaatcagaa aaaaagttgt tcctgctgtg
     1501 atagttaaga aaccttttta taaaaaacaa tataaaaaag ataatattat attaaacaaa
     1561 gaaaataaat tttcatatat acctgctaca agtgaagata aatcaaaaat gttaaaagta
     1621 gttggattaa attcggttga tgaattattt tcagatatac cagaagaagt aaaattaaaa
     1681 agagatttaa atttggaaat tggaaaatct gaattagagg taagcaaaat agtaaaaaga
     1741 ttgtcagaag aaaatttaag tttagaagac ttgacttgtt ttcttggtgc tggtgcatat
     1801 gaccattata taccatcaat tataaaacat ataacttcaa gatctgaatt ttatacagca
     1861 tatactccat atcaagctga aataagtcag ggaactttac aagtagtttt tgaatttcaa
     1921 tctatgattg cagaaattac aggtatggaa attgcaaatg catcaatgta tgatggagca
     1981 actgctgcaa tagaagcatg tataatggca atgaatcaga ctagaaaaag taaaatagta
     2041 gtgtcaaaaa ctattcatcc tgaaactttg tcaatattaa gaacttactt acaatataaa
     2101 gattgtgaga tagtagaaat tgacttttgt aatgaatatg gcactacaga tatagaaaaa
     2161 ttaaaagctt ctgtagacaa agatacagca tgtgttctta tacaaactcc taatttcttt
     2221 ggaattattg aagaaatgga agaaatagaa aagataactc atgaaaataa ggctatgtta
     2281 attatgagtg tagacccaat atcattagga gttttaaaaa caccaggtga gataggtgca
     2341 gatattgttg ttggagaagc tcaatcactt ggaaaccctc ttaattttgg aggtccttat
     2401 gtaggatttt tagctagcaa atctaaatat actagaaaaa tgccaggaag aatagttggg
     2461 caaagtttag atgtagaagg taaaattgca tatgtattaa ctttacaaac tagagaacag
     2521 catgtaagaa gagaaaaagc aacttctaat atatgttcaa atcaagcttt aaatgctctt
     2581 gtagcttcaa tatatatagc tactatgggt aaagaaggat ttaaagaggt tggtatgcag
     2641 tctatgaaaa aagcacatta tacttacaat aagcttgtac aaactggaaa atataagcca
     2701 atatttaaag gtaagttttt caaagaattt gctgtacaag gaaatcttaa tatagaaact
     2761 ataaatgata aactcttaga agaaaatata ttaggtggtt ataatttaga atacaattat
     2821 ccagaattaa aaaattcaac tttgctttgt gtgactgaga aaagaagcaa agaggaaata
     2881 gataagttag ttggaataat ggagggatta taatgaaaga atacaatagt ttgttgatag
     2941 atatatctaa aaaaggtagg aaagcatact cacttccaaa gttagatata gatgatataa
     3001 aaatagaaaa tatgattgac caagatatgg caagacaaag tgagttgaat ttgccagaag
     3061 ttggtgaatt agaattagtt agacattata cattattatc aaataaaaac tttggagttg
     3121 atacaggttt ttatcctttg ggttcttgta ctatgaaata taatcctaaa ataaatgaag
     3181 atatggcagc acttccaaac tttactggaa tgcatccata ccaatcttcg gatacagctc
     3241 aaggttctct ttctttgatg tatgatttat caagaagact tgcagaaatt acaggtatgg
     3301 atgaagttac actacaacct tcagcaggtt ctcatggtga gtttacaggg cttatgatta
     3361 taaaagcata tcatgaaaat agaggtgacc ataagagaac caaagtgata attccagatt
     3421 ctgcacatgg aacaaatcca gcaagtgcag ctatggcaaa ctttgatgtg attcaaatag
     3481 catctgataa aaatggagct gtagacataa atgcattaaa agaggttttg aatgatgaag
     3541 tagcagctct tatgcttact aatccaagta ctcttggttt atttgaaaaa aatataaaag
     3601 aaatagctac tcttgtacat gaagcaggag gacttttgta ctatgatggt gcaaacatga
     3661 acgctattat ggggataaca agacctggag atatggggtt tgatgtggtt catcttaatc
     3721 ttcataagac tttttcaact cctcatggag gtggaggtcc aggagctggt cctgttggag
     3781 ttaaaaaaga attagtacct tttttaccaa taccagtaat tgaaagaaaa gaagataggt
     3841 atgtactaaa ttatgataga gaaaaatcta ttgggaaaat taaaaatttc tatggtaatt
     3901 ttggagttct tgtaagagct tacacatata tactaactat gggaagggat ggacttaaag
     3961 aggctagtga aatggcagtt ttaaatgcta attatataaa ggaaagtata aaagatgatt
     4021 atattttgcc aatagatacc ttgtgtaagc atgagtttgt attaggtgga ttgccaagag
     4081 gagaggccaa tataaaaact atcgatattg caaaaagact actagattat ggataccatc
     4141 ctccaacaat gtactttcct cttattataa atgaggcact tatgatagaa cctacagaaa
     4201 gtgagagtat agagacatta gatagtttta tagaagctat gaaaagtgtt gctaaagagg
     4261 caaaggaaga accagaacta ttgaaaactg ctccacataa tactttggtc aaaagagtag
     4321 atgatgcaag ggcagtaaag aaacctatac taacatggtc acaaagataa atagttaaaa
     4381 agtagagatt taaagtataa agagaattaa atttgtaaga aaaagctatc taacttaatc
     4441 tagtttagat agcttttttg tatattagta tgtatggtat tttctatata aagttttaaa
     4501 ttatattagt gcttatatag aatattgata tgatgattat gtatagatat taataagaat
     4561 tgttgaattt atcttgcctt aactttattt actcttttat attaa
//
LOCUS       DACXMZ010000256         4583 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.257, whole genome shotgun sequence.
ACCESSION   DACXMZ010000256 DACXMZ010000000
VERSION     DACXMZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.257"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            159..3698
                     /gene="nifJ"
                     /locus_tag="JD016_17365"
     CDS             159..3698
                     /gene="nifJ"
                     /locus_tag="JD016_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAU5001385.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            3940..4068
                     /locus_tag="JD016_17370"
     CDS             3940..4068
                     /locus_tag="JD016_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001386.1"
                     /translation="MLYTLKLREQIIFIVCIFGFDNFKIDTKEYLICVFYHLEILF"
     gene            complement(4090..>4583)
                     /locus_tag="JD016_17375"
     CDS             complement(4090..>4583)
                     /locus_tag="JD016_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HAU5001387.1"
                     /translation="KPDNNKPESNKPEDNNNSDSTNKPDDNNNSGSTSESFSAYQKEV
                     VDLVNIERAKAGLNPLTLDSSISNVATKKSQDMIDNNYFSHNSPTYGSPFDMLKKFGI
                     SYKTAGENIAMGQKTPKEVVNAWMNSEGHRKNIMNPNFSKIGVGVAQKSGGSIYWTQI
                     FVG"
BASE COUNT         1625 a          638 c          858 g         1462 t
ORIGIN      
        1 aaggaatttt atctaatctg tagtattata ttagaagata gagtatttgc gcgactatat
       61 gtgtgtttat atttttgttg tgtaaattat atgtaaatat aaaaaaatag acttgttata
      121 aacaagtaca attgtagcta tcaaggagga tacatattat ggctaagttt atgaaaacac
      181 ttgatggaaa tacagctgca gctcatgttg cctatgcgtt tacagatgta gcagctatat
      241 atccaatcac accatcttca actatggctg aagtagttga tgagtgggca tcacaaggaa
      301 gaaaaaatat atttggacaa acagttaatg ttgttgaaat gcagtcagaa gcaggtgcat
      361 caggaacatt ccacggttct ttacaaggag gagctttaac ttctactttt acagcatctc
      421 aaggtttatt attaatgata cctaacatgt ataaagttgc aggagagtta ttaccaggag
      481 tattccatgt aagtgctcgt gctttagcat ctcaagcgtt atcaatattt ggggatcacc
      541 aagacgttat ggctgctaga caaactggat gtgtattatt agcttctggt tcagttcaag
      601 aagtagctga tatagcacct gttgcacatt tagctgcaat agaaggtaga ttaccattta
      661 tacatttctt tgatggattc agaacttcac atgaaatcca aaaagttgaa ttactagaaa
      721 atgaagatta tgcaagttta ttaaactttg aagcagttca agcatttaga gataatgctt
      781 tatctccaaa tcatccagtt gctcgtggta ctgctcaaaa cccagatata tatttccaaa
      841 ctagagaagc ttctaataaa tactatcaaa atatagtagg tatagttgaa aaatacatgg
      901 aaaaaatgag taatttaaca ggtagaaaac atagtttatt tgattactat ggagcagaag
      961 atgctaaata cgtattaata gctatgggtt cagtaacaga aacaatagaa gaaactatag
     1021 attacttaaa tgctaaggga gaaaaatatg gtttagttaa agttcattta tatagaccat
     1081 tctcaatgaa acatttctta gatgctatgc catctactgt tgaaagaata tgtgtacttg
     1141 atagaactaa agagccaggt tcaactggtg aaccattata cttagatgta cgtgatgtat
     1201 tctatggaaa agaaaatgct cctatgataa taggtggaag atacggatta ggttcaaaag
     1261 atactactcc ttcagatata aaaactgttt ttgataactt agtttcagaa caacctaaaa
     1321 atggatttac agtaggtata gttgatgatg taactaatac ttctttaact ccatctgagc
     1381 caataaaaat agcttctaaa ggaactataa gatgtaagtt ctggggatta ggttctgacg
     1441 gtactgttgg agctaataaa caagctatca aaataatcgg tgaccatact gaaaaatatg
     1501 ctcaagctta ctttgattat gactctaaaa aatctggtgg tataactatg tctcacttaa
     1561 gatttggtga tactccaata agagcgactt atttaataga tgaagctgat tatattgcct
     1621 gtcataaaca atcatatgtt tatcaatatg atttattaaa aggtcttaaa aaaggtggta
     1681 catttgtact taacactata tgggaccaag caggacttga agaacattta ccagctcata
     1741 tgaaacaata tatagctaaa aatgatataa aattctatac agttaacgct gttaaactag
     1801 gacaagaaat aggtcttgga aacagaataa acatgataat gcaatctgca ttctttaaat
     1861 tagctgaaat aataccagaa gaagatgctg ttaaatactt aaaagattct atagttaaag
     1921 cttatggtaa aaaaggtgaa aaaatagtta acatgaacta tgctgctgtt gatgcaggaa
     1981 taaatgcttt agttaaagtt gaagttccag cttcttggga aaatgctgtt gatgcagata
     2041 aaggagaagt taaagaacca gaatttataa aaaatatact aagaccaatg actgcacaag
     2101 aaggaaacaa cttaccagta agtacattca atggtataga agatggaaca ttcccttgtg
     2161 gtactgcagc ttatgaaaaa cgtggtatag ctgttgatgt tcctgaatgg ataatagata
     2221 actgtattca atgtaaccaa tgttctttta tatgtcctca ttcatgtata agaccagtat
     2281 tagttactga agaagaatta gctaatgctc cagaaggatt tgaagctaaa aaagcattag
     2341 gtaaaggatt agaaggatta aaatatagaa tgcaacttag tccacttgac tgtacaggat
     2401 gtggaaactg tgctgacata tgtccagcta aagaaaaagc tttagttatg aagccaatag
     2461 atactcaact tgatacagag ttagataatt gggcatttgc agtaaatcct gatgaggtag
     2521 ctccaaaagg tgatgttatg cctgcaaaca ctgttaaagg aagccaattt agacaaccat
     2581 taatggagtt ttcaggagca tgtgctggat gtggagaaac tgcatacatt aaagtagtta
     2641 cacaattata tggagataga atgatgatag ctaatgctac tggatgttct tctatatggg
     2701 gagcttctgc accatcaatt ccttatactt gtaaccatga aggtaaaggt ccatcttggg
     2761 caaactcttt attcgaggat aatgctgaat atggatttgg tatgtacaca gctgttaagc
     2821 aaataagaaa taaaatagta gatgctatga cagagttagt ttctatggat atctgtgaag
     2881 atgctaaagc agtatttaca gagtggttag attctagaaa tgatggagaa gcttctaaag
     2941 tagcaagtgc taaggttgtt gaattattag aaaaaccagc ttgtgattgt acagatgaaa
     3001 aagctaaaga attagtaaaa gctataaaag atagaaaaga ttatctagtt aagagatctc
     3061 aatggatact aggtggagac ggttgggcat atgatatcgg ttacggtgga ttagaccatg
     3121 ttcttgcatc tggtgaagat gtaaatgtac ttgtatttga tacagaagta tattcaaata
     3181 caggaggtca agcttctaaa gctactccag ctgctgctat ggctaagttt gctgcttcag
     3241 gtaaaaaatc tagaaagaaa gatttaggaa tgatggctat gacatatgga aacgtatatg
     3301 tagctcaagt tgctatgggt gctgataaaa accaatttat caaagccgtt atagaagctg
     3361 aaaaacatga tgggccatct ttaataatag cttatgctcc atgtataaac catggattaa
     3421 aagaaggtat gggaagaact gtagctaacg aagcacaagc tgttgcttgc ggatactggc
     3481 atttatacag attcaatcca gaagtaaaag ctgaaggtaa aaacccattc actttagatt
     3541 caaaagaacc aactgcaagc ttcaaagatt tcatacttgc acaagtaaga tattctgcta
     3601 tagctaaaca attcccagag gaagctgatg gattatttgc taaagctgaa gctgatgcta
     3661 aagaaagata tgaaggatac aaaaaattag ctgaataata ttaaagtatt ataatgctta
     3721 tatagaattg ataaaagaga gtgtctcaaa gtgagctttt tagttcatga ggcactcttt
     3781 ttgtatgaaa tcaaatatat tttaatcatt tcaacaatga aaaaggcctt atctctattt
     3841 tgagacagcc tttttttatg aagatattaa gatcctgttt ttacctatta aatatctttt
     3901 taagttttcc attttcatct tgccaatact ttttatatat tgctctacac actaaaatta
     3961 agagaacaaa taatatttat agtatgtata tttggatttg ataattttaa aatagataca
     4021 aaagagtatc tcatttgtgt tttttatcac ttagagatac tcttttgata tgtatggaaa
     4081 aagagtattt tatcctacaa agatttgtgt ccaataaatt gaaccaccac ttttttgtgc
     4141 tactccaaca cctattttac tgaaatttgg attcattata ttttttctgt gaccttcaga
     4201 attcatccat gcatttacaa cttctttagg agttttttga cccatagcta tattttcacc
     4261 tgctgtctta tagcttattc caaatttttt caacatgtca aatggagaac cataagttgg
     4321 agaattgtgt gaaaaatagt tgttatcaat catatcttga gatttttttg tagcaacatt
     4381 tgatatagaa gaatcaagag taagaggatt taatccagct ttagctcttt ctatatttac
     4441 taagtcaaca acttcttttt ggtaagctga aaaactttca cttgtagagc ctgagttgtt
     4501 attatcatca ggtttgttag tagagtctga attattgttg tcttcaggtt tattagactc
     4561 aggtttattg ttatcaggtt tat
//
LOCUS       DACXMZ010000257         4565 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.258, whole genome shotgun sequence.
ACCESSION   DACXMZ010000257 DACXMZ010000000
VERSION     DACXMZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.258"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            105..1463
                     /locus_tag="JD016_17380"
     CDS             105..1463
                     /locus_tag="JD016_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001388.1"
                     /translation="MNPFVLMFAAVITGILFGKIKFGKFNFGVSGALFTGLFIGWLAY
                     SLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAAVGLLAAKDMKAVLKK
                     YGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTGALTSSPGLAAALETA
                     GKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQEQIDKYIAYAEAGVGI
                     GHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEARDSVSGKTIPEVPFNI
                     ITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGYIGKIGVVNFRMEEKV
                     LNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVGVTAMMIGFLVGKYVF
                     KLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPFALLTKVILVIVLHKL
                     PM"
     gene            1490..3034
                     /locus_tag="JD016_17385"
     CDS             1490..3034
                     /locus_tag="JD016_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HAU5001389.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            3065..4456
                     /locus_tag="JD016_17390"
     CDS             3065..4456
                     /locus_tag="JD016_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAU5001390.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIVKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
BASE COUNT         1748 a          508 c          937 g         1372 t
ORIGIN      
        1 tattggggag aaaggagaaa tatatttttt aataatattt gttaatttaa taacgaaaaa
       61 tgaaagggag tgatttagtg aagtttgaat tgatggattt tttaatgaat ccttttgtcc
      121 ttatgtttgc tgcagtaatt acaggaattt tatttggaaa aattaaattt gggaagttta
      181 attttggggt atcaggagca ttatttacag gattatttat tggatggctt gcatatagct
      241 tggggaatct aataatagca aaaggggaaa cagcagcagg atataaggca gcaactgtaa
      301 tgatggggaa tggaattatt tcttctgact tctttgattt ctttttaata atatttgttg
      361 cagcagttgg tctattggca gcaaaagata tgaaagctgt attaaagaag tatggagcaa
      421 gatttgtaat tttaggagtt cttataactt ttataggtgg ctctatgact tatgcaatga
      481 ctttactatc aagtgataaa ggaagtagtg cttatgaagt ttctggcgtt tacacaggag
      541 cattaacaag ttcgcctgga ttagctgctg ctttagaaac agctggtaaa catgcagaag
      601 atgtatcaaa agaatttgaa aaagcatcaa taaaagataa aaaagaaatt ttaaaagtag
      661 tcgacccaga aggcaaattg gatgtgaata caactacatc actgactcaa gagcaaatag
      721 ataagtatat tgcatatgct gaagctggtg ttggtatagg tcatgcagtc gcatatcctt
      781 ttggagtttt aatagtaatc ttaggtgtaa atttccttcc aaaattattt aggatggacc
      841 taaaagaaga aagaagaaaa tatgagaagg aaatgaagga agcaagagat agtgtttctg
      901 gtaaaacgat accagaagtt ccatttaata taataacatt tttcttgaca tgtttagctg
      961 gatacttagt tggaggaata catgtattta tgggaccact agggtacttc acgctaggtg
     1021 caacaggagg ttcactaatt gtatctctag ttcttggata tataggtaag ataggggtag
     1081 taaatttcag aatggaagaa aaggttttaa atattttaaa acaaataggg ctagtatttt
     1141 tcttagcaat agttggatta agatatggtg gaaaagtagt tgattcaatt atgacatctg
     1201 gtatgcatct agccttagta gcaatagcag taggtgttac agctatgatg ataggattct
     1261 tagttggtaa atacgtattt aaattaaatt ggattttatt atctggagct gtctgtggtg
     1321 gtatgacatc aacaccaggt cttggagctg ctgttgatgc tcttgatagt gatgacccag
     1381 cagcaggtta tggtgctacg tatccatttg cattattaac taaagttata ttggttatag
     1441 ttttacacaa acttccaatg taaataagat tttatatggg gggatattta tgaataatga
     1501 aggagcattt gaagaattaa gtggaagatt aagaaataaa gaattgtgtg atttaataat
     1561 gaatgcagat gaagctgcta aaattataga ggatggcatg gttattggag ttagtggatt
     1621 tacaccttca ggctatccaa aagcagtacc attagcagtt tcagaaagag ctaaaagtgg
     1681 agaaaagata aaattaaccg tatattcagg agcatcttta ggtccagaag ttgatggagc
     1741 atggtcagaa gcaggtataa ttgaaagaag acttccttat caaactaatt caatacttag
     1801 aaataatatt aataaaggtg ttgttgatta tatagatatg cacctaagtc attctactca
     1861 atttttaaat tatggaacta tacctaaagt agatgtagct atagtagaag cacttgcaat
     1921 aactgaagaa gggaatatta taccaactag tgggataggt aattctccat cttttataaa
     1981 aagtgcagat aaagtaatag tagagataaa tcttgcaaaa ccaatggaaa tggaaggtat
     2041 ggcagatata tatattacgg aaaatccacc aaatagaaaa ccaatagaaa taaatcatcc
     2101 taaggataga attggaacaa catatatacc ttgtggtttg gataaaattg caggagtagt
     2161 aattaccaat atgcaagata agacccgtcc attgggggtt gtagatgaag cttcgaaaaa
     2221 aatatcaaat aatattattg cttttttaag agaagaagta aaggcaggca gattatcaaa
     2281 gaatttactt cctcttcaat ctggtgtagg aagtgtagca aatgcagtat tatatggatt
     2341 atgtgaatct gaatttgaaa atttaacttg ttatacagag gttgtacagg actctatgtt
     2401 ggatttaata agaatgggta aagttacaat ggcatctact acatctgtaa gtccatctcc
     2461 agaaggatta ataaaatttg aaaaagatat tgattttttt aaggataaga ttatattaag
     2521 acctcaagaa attagtaaca atccagaaat agccagaaga attggtgtaa ttgcaatgaa
     2581 cactgcaatt gaagttgata tttatggaaa tgtaaattct actcacataa tggggtcaaa
     2641 gatgatgaat ggaattggag gttctggtga ttttgctaga aatggagcaa taactatatt
     2701 tagtactgag tcaattgcaa aaaatggtga tatatcatca atagtaccta tggtatctca
     2761 cgtagaccac actgaacatg atgtaatggt aattgtaaca gaacaaggat atgctgattt
     2821 aagaggtctt gcacctagag aaagagctat aaaaataata gaaaattgtg cccatccaga
     2881 ttataaagaa caacttaggg attatttaaa tagagcttgt aaaagtggag caaaacaaac
     2941 acctcacata ttggacgaag ctttatcatg gcatagtaaa tttatgagta caggtacaat
     3001 gaaaaaggct gaaactttta aaagtgcatt ataaaatatt taataaattg gaggtagatt
     3061 ttaaatggaa aaagcagtgg aaaactttga agatttaagt aaggaataca ttaatggtta
     3121 tattgagaga gcaagaaaag ctcaaagaga gtttgaatgt tatactcaag aacaagtaga
     3181 taaaatagta aaaatagttg gaaaagtagt gtattataat gctgaatatt tagctaaact
     3241 tgctgtagaa gagactggaa tgggagtata tgaagataag gtagctaaaa ataaaagcaa
     3301 agcaaaagtt atatataaca atttaaaaga taaaaaatca gttggtataa ttgatataga
     3361 tagagaaact ggaataacaa aggtagctaa accagttgga gtagtagcgg caataactcc
     3421 ttgtacaaat ccaatcgtta cacctatgag caatgcaatg tttgcactta agggaagaaa
     3481 tgcaattatt atcacacctc atcataaagc aattggatgt agtacaaaaa ctgtagagat
     3541 gattaatgaa gaattagaaa aaataggagc tccagaaaat ttaatacaaa tacttgacca
     3601 acaatcaaga gaaaatacaa gaaatttaat ttcatcagca gatgtagtaa ttgccactgg
     3661 aggaatggga atggtaaaag ctgcgtactc aagtggaaaa ccagcacttg gtgtaggggc
     3721 aggaaatgtt caatgtatca tagatagaga tgtagacatt aaagaagcag tacctaaaat
     3781 catagcagga agaatttttg ataatggtat catttgttca ggtgaacagt cagtaatagt
     3841 agcagaagaa atgtttgata aaataatgga tgaatttaaa aataacaaag gttttatagt
     3901 tagagataaa gtacaaaaag aagctttcag aaatgctatg tttgtaaata aatctatgaa
     3961 taaagatgca gtaggtcaat cagttcatac aattgcaaaa attgcaggtg ttgagatacc
     4021 agaagataca aaaataatag taatagaagc tgatggacca ggagaagagg atattattgt
     4081 aaaagaaaaa atgtgtccag ttatatctgc atacaagtat aaaagttttg aagaaggtgt
     4141 agctattgca aaagcaaatt taaatgtaga aggaaaagga catagtgtat ctatacattc
     4201 aaatacagtt aaaaatatag aatatgcagg agaaaatata gaagtatcaa gatttgttat
     4261 taatcaatgt tgtgctacta gtgctggagg aagtttcttt aatggtcttg cacctactaa
     4321 tactctaggt tgtggttctt ggggaaataa cagcatctct gaaaatcttg attataagca
     4381 tctaattaat atttctagaa ttgcttatta tatgccagaa aatgaagtcc ctacagatga
     4441 agaactttgg ggataaaatt tagacaaaaa atattcattc taaattaaag taggaatata
     4501 ttttaattta gaatgaattt aagacataaa ttaaatttaa attagtttat tggactaaat
     4561 ttaaa
//
LOCUS       DACXMZ010000258         4524 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.259, whole genome shotgun sequence.
ACCESSION   DACXMZ010000258 DACXMZ010000000
VERSION     DACXMZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.259"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            145..1143
                     /locus_tag="JD016_17395"
     CDS             145..1143
                     /locus_tag="JD016_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HAU5001391.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            1505..2032
                     /locus_tag="JD016_17400"
     CDS             1505..2032
                     /locus_tag="JD016_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HAU5001392.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            2059..2655
                     /locus_tag="JD016_17405"
     CDS             2059..2655
                     /locus_tag="JD016_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HAU5001393.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIDVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(2720..4303)
                     /gene="asnB"
                     /locus_tag="JD016_17410"
     CDS             complement(2720..4303)
                     /gene="asnB"
                     /locus_tag="JD016_17410"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAU5001394.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     YERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(4368..4505)
                     /locus_tag="JD016_17415"
     CDS             complement(4368..4505)
                     /locus_tag="JD016_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001395.1"
                     /translation="MKNKKFNVITLIILDNHIYAINKEQISTNSKRIYFEKSRFFSDY
                     K"
BASE COUNT         1591 a          699 c          651 g         1583 t
ORIGIN      
        1 atcaaaataa cacagggaaa acaatttcaa aactaaattt ttaaccttaa aaattttgta
       61 taaaaaatat aaccttatat gctacatatt atttctaaat gaaaaattac cataataatt
      121 taataattac aggagggtat tgatatgaaa atactagtat ttggagcacg cgattatgaa
      181 gaaccagtaa taaaaaaatg gtctgaagaa cataaggatg ttcaagtgga tatttatcct
      241 gaaaacatga ctgaagaaaa tgtagttaaa gctaaagggt atgatggtat atctatacaa
      301 caaactaact atatagataa tccttatatt tatgaaactt taaaagatgc tggggttaaa
      361 gttatagctt caagaactgc aggggttgac atgatacatt ttgatttagt taatgaaaat
      421 ggacttatcg ttacaaacgt tccttcttat tcacctaatg caatagctga attagctgtt
      481 actcaagcta tgaacctttt aagaaagact cctctagtaa agaaaaaagt ctgtgaaggt
      541 gattaccgtt ggatagctga acttcttgga acagaagtta gatctattac agttggtgtt
      601 ataggtacag gaaaaatagg tgctacttct gcaaaattat tcaaaggcct aggagctaat
      661 gtaattgcat ttgaccaata tccaaatagt gatttaaatg atatattaac ttacaaagat
      721 tctttagaag accttctaaa agaagctgac cttataacat tacatactcc tttacttgaa
      781 ggaacaaaac atatgataaa taaagatact ctagctataa tgaaggatgg agcttacata
      841 gtaaatactg gccgtggtgg tttaattaat acaggggatt taatagaagc actagagtca
      901 ggaaaaatta gagctgctgc ccttgataca tttgaaactg aaggattgtt cttaaacaaa
      961 aaaatgaatc ctggagaatt aactgaccca gaaataaata aacttctttc tatggaacaa
     1021 gttatattca ctcatcacct tggtttcttc actagtacag cgattgaaaa tatagtttat
     1081 tctagtttaa gtagtgctgt agaagttata aaaacaggaa ctgctactaa tagagtaaat
     1141 tagtatttta taaatatatt ttttcattaa ttagctatta tctaacaaaa tagcctttaa
     1201 tttcccaaat ataagagagt cagctcagta tatattgagt agactctctt atattatttt
     1261 ttatattaaa cttgtactct tcttattcat accaaattaa atttatatta acttattcta
     1321 actactccta ctctatatat tttacctatt tttacttttc taattatata catttgaatg
     1381 ggtacaatct gtattttaca aaaaaaacac ctaacaaact atgaaaattg tatgattaat
     1441 attaatatat ttataagaaa acataacaat tatgttagct ctggattttt agggagggag
     1501 tatattggaa aaaatgaata aagttacttc taaaaaaact acctatattg ttacttcagc
     1561 attatttgca tccataatat gcctgacaat agcatatata ttacatattc cagttggtgg
     1621 aaacaatgga tatgttcata taggagatgc ttttatttat ctagctgcaa ctattttgcc
     1681 aacaaactat gctatagctg cttctgcaat tggtgctggt ctagctgatt tatctacagg
     1741 tgctgctatc tgggtaatac caactataat cataaaacca atacttgtat tatttttcac
     1801 ttctaaaagt gacaagataa taaataagag aaatatagtt gcttcggttg tagcaggtat
     1861 agtaggatta gttttataca tgtttgctga aggtataatt attggaagtt ttacaagtgc
     1921 atttgtaatg agcttacttg gcctattaca gccaatagga agttttattg tttttataat
     1981 cttaggtatg gcacttgata aacttgattt taaaaaacgt tattttaatt aagaatttaa
     2041 atttatggag ggaaaattat gaatatctta tatacaattg aaaatagtat ctatgtaaat
     2101 ataactaata catgtccatg tagttgtgtt ttttgtataa gaaacgaaaa agatgaagtt
     2161 gcaaatagtg gtagtttatg gttggagcat gagccaagtg tagatgaagt aaaagaagcc
     2221 ttcaataaat acaatttaga cgactatgat gaaatcgtat tttgtggata tggcgaacca
     2281 ttaatgagaa taaatgaatt gatagatgta gcaaagttta taaaagaaaa aagtagcata
     2341 aagataagaa taaatacaaa tggtctaagt gatttaatac ataacaaaaa aacagctatt
     2401 ctgttaaaag atgttataga tgcagtatca ataagtctta atgccccaaa taaagaagct
     2461 tataatagag ttactcaacc aaagtttgga gaaaaatcat ttgattatat gttagatttt
     2521 gcaagagatt gcaaaaaata tataaaagaa gtagcttttt cagtagtaga tgaaatatct
     2581 ccagaagaaa tagaagaatc taaacaactg gcaaaaaaac ttgatatacc attaagagtt
     2641 agacataaga attagcaagt aaaattataa aacattaaga atcacctgaa tggtctttca
     2701 ggtgattctt cctcttgtat taacatttta caaaatagat ttcataccac actaggaatg
     2761 tgtaaatcat ccaaatcttt ctccaattat cagagtttcc actaatgtag tcattaaaaa
     2821 tctcattaat ctcatctatg ttaaagaatt tatctgctat ctcactattg aatttatctc
     2881 ggatatcctt attatattgg tcatcagcca tccatatacg aattggcaca ataaatccta
     2941 atttatttct aaaagcaatc tcttctggta atgtttttga tgcagctgta cgaaaggcaa
     3001 ttttattctc ttttcctttc actttatatt ctgatggtat tctggatgca atatcaaata
     3061 ttcttgtatc tgtaagaggc attctgactt ccaatccaac tgcagtactc attttatcta
     3121 tattaaaata aatatctcct tccaaccata tttgaatatc tacatttgac atgttagtaa
     3181 gtgtatctag ttcttctgtt tcttcataaa ttccttttac cacttcaata ggaagcacat
     3241 caggattgta tttcttaaga atttttcttt tctcaccctc attcataatg tttgtatttc
     3301 caatataaaa tgtcttcaaa ttctctccat aacactctgc cttatggtat atattatatc
     3361 caccaaaaaa ctcatctgct ccttctccag agtaacagat atttgtgttt tcagctgctg
     3421 ccttgcatcc aatagcaaaa acgatagcag atgcatctcc agttggttgc tccatatggt
     3481 acattacata aggtactgac gcaaaatact cttcaggtgt aattatatat ctcgaatttt
     3541 tacacccaag aatattagct gtctgttgtg ctaattttga ttcatcaaaa cgctcttcct
     3601 cataaccaca tgaacctgtt ttttctatat ctgacattgc caatatataa gatgaatcaa
     3661 cacctcctga taagaataac tctgcagttt cattctctga ttttacctct ggcataattt
     3721 tctgaagtgt tgtatgaatt tcatctgtcc attcctcaat agacttgctg tggtcaggat
     3781 gaaattcagg tttccaataa cgaccaatac atactgtttt attctgatag acaaggtaat
     3841 gtcctggcat aagcttcttt actccacgga agaatgtatt ttcacctgct acatatgtga
     3901 aagacatata aatctgtaac atgtcttcat ttaactcttt cacaaatcct ggttgctcca
     3961 taatcttacg aatagatgaa ccatagagaa gcttcccatt ttcagtctca taataataga
     4021 aaggttttac tccaaactgg tctcttaggc agaagagttt cttttccaca gtatcccata
     4081 atgcaaaagc aaacattcca tgcatgtgat ttgccatatc atatccccat gtcttatatg
     4141 cttccactaa aattgctcgt tcacgctcat atctacataa tgaagaatcg attccaagtt
     4201 cttcacaaag aactttccag tttcgaatat gtccattgta ttctagaatt tttatcatat
     4261 atgtgtcttt tcgtgttaaa tacttttctt ctaaatttat catatgtgta tcctttcgta
     4321 ttaaaacatt taacttctaa atttattaaa tgtttatata tatttattca cttataatca
     4381 ctgaaaaatc gacttttttc aaagtaaatt ctttttgaat tagtactaat ctgctcttta
     4441 ttaatggcat aaatatgatt atcaagtatt atcagtgtta taacattgaa ttttttattc
     4501 ttcataaata ttgccccctt tata
//
LOCUS       DACXMZ010000259         4502 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.260, whole genome shotgun sequence.
ACCESSION   DACXMZ010000259 DACXMZ010000000
VERSION     DACXMZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.260"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1439
                     /locus_tag="JD016_17420"
     CDS             <1..1439
                     /locus_tag="JD016_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAU5001396.1"
                     /translation="LSDAEAASEKLSDRSEADRLKAACEASKAVCTSLVKEKKTVAPP
                     KLFDLTSLQREANRLFGYTAKQTLDLAQALYEKRLLTYPRTDSSYLTDDMGDTAAGII
                     KLLCGKFPFMAGKDFTPELGKVLNSKKVSDHHAIIPTMELAKTDLAALPESEKNILTL
                     AGARLLMATAAPHTFEAVTAIFECAGQSFTARGKTVLSDGWKEIDRKYRAALKNKPEA
                     DNADSDTEKTLPPFTEGQTFENPAATVTEHDTTPPKPHNEASLLSAMERAGSEDTDPD
                     AERKGLGTPATRAAVIEKLVKGGFVERKGKQLLPTKDGINLVCVLPDTLTSPQLTAEW
                     ENNLTQIAKGKADPAVFMEGIEDMARELVKTYPFLSDDKSQMFKPEREALGSCPRCGS
                     PVYEGKKNYYCSNKECIFTMWKNDRFFEERKVAFTPKIAAALLKSGKVNVKKLYSPKT
                     GKTYDGTIVLADTGGKYVNYRVELPKKK"
     gene            1681..>4502
                     /locus_tag="JD016_17425"
     CDS             1681..>4502
                     /locus_tag="JD016_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAU5001397.1"
                     /translation="MASLRDTVKDYQEELRDGIAWVAFWKMGRSWNAEYFHLEMGDYL
                     YPEDRSRMEEIKQADPAAVVVNGYYSGYLGEDRNLDELTAGVRRHYENGYSNIGEFIE
                     AHDDRLPPELIEEARDAAHAAGLPFSEKAYRDGEEPDPYLFDGSMSMEDYELMHRMIE
                     NERSERMEETILSGYLSNLGKYTEGRPAGEWVSFPTTAEHLKEIFDRIGIDGKNYGEL
                     HITEYQSSIAGLAGKLTELESLDELNYLGELLKMQFDDDREKFVAAMAYGDHTRNLQD
                     IINLAQNLDCYWLYPSVQSEEDYGHYLIEELDELELPEEAKKYFMYEEYGRDAAINDG
                     GRFTEQGYIYNNRNTFTQWYDGRDVPEEYRVTPQPPVQEKEQADLDAAAVQPAFATEQ
                     PPVLPIILSSEKPADKMKEITDRLEQGILGIYESDRYADYLRTMSKFHNYSFNNTILI
                     AMQGGNLVKGYKQWEKEFDRHVKPGEKAIKILAPSPFTVKKQVEKIDPDTQKPVFDKD
                     GKPVTEEKEVKIPAFRVVSVFDISQTEGKELPALTYELTGNVEQYKDFFAALEKTSPF
                     VMGFETLSGGVKGRCNYEEKRIIINEGMDELQNIKTAIHEIAHATLHDTALAMPERPD
                     RRTREVQAESVAYAVCQHYGLDTSDYSFGYIAGWSSGKELAELKGSLETIRSTAAKLI
                     DTIDGHFAEIQKAQDKEQTTEQAQPAQEAAKQPEAEAAAPEVPEEIAPAQEKEAQTEP
                     EADTGASSEAPQPPQPEQATPAAPYYPINEAAAKRAKDMNSFSDYKQGSATAEYRHYV
                     DEAVQLAERQKQRVDPMYHDKIDSLLDTYARKLAANMNKGYEIDARVPSILIAGGSNF
                     PTRKKEKQNAARDSNYREWQDIQGLLDKIRSTGMGGISADDPQAVQKLEKKLESLEKS
                     QETMKAVNAYYRKHKTLDGCPHLSPE"
BASE COUNT         1325 a         1113 c         1177 g          887 t
ORIGIN      
        1 ccttatccga cgcggaagct gcaagtgaaa aactgtctga ccgttccgaa gctgacaggc
       61 tgaaagctgc ttgcgaagcg tcaaaagcag tctgcacttc ccttgtgaaa gagaaaaaga
      121 ccgtagcccc tccgaagctc tttgacctta cttctttaca gagggaagca aaccgcctgt
      181 tcggatatac ggcaaagcag acccttgacc ttgcacaagc cctgtatgaa aaaagacttc
      241 ttacttatcc gagaacagac agcagttatc ttactgacga catgggcgac acagcggcgg
      301 gcattatcaa gctgctttgt gggaaatttc cttttatggc gggaaaagac ttcacgccgg
      361 agcttggaaa ggtgctgaac agtaaaaagg tatcagacca ccatgctatt atcccgacta
      421 tggagcttgc aaagaccgac cttgccgcgc tgccggaaag cgaaaaaaat atccttactc
      481 ttgccggggc gcgtctgctt atggcgaccg ctgcaccgca tacctttgaa gcggttacgg
      541 cgatttttga atgtgccgga caatctttta ccgcaagggg aaaaacggtg ctgtccgacg
      601 gttggaaaga gattgaccga aaatacaggg cagccttaaa gaacaagccc gaagcggaca
      661 acgcagatag cgacactgaa aagaccttgc caccatttac cgagggacag acctttgaaa
      721 atccggcggc aacggtaacg gagcatgaca ccacaccgcc aaagccccac aacgaagcgt
      781 cgctgctctc tgctatggag cgcgccggaa gtgaggacac cgacccggac gcagaacgca
      841 aagggcttgg aacgcccgcc acccgcgctg ccgtcattga aaaactggtt aagggcggtt
      901 ttgtggaacg aaaaggcaag cagctacttc ccacaaagga cggtatcaac cttgtgtgtg
      961 tcctgccgga cactctgaca agcccgcagc ttaccgcaga atgggaaaac aatctgacgc
     1021 agatagccaa aggcaaggca gaccccgccg tatttatgga gggtattgag gatatggcgc
     1081 gggaactggt aaagacctat ccgtttcttt ctgatgataa atcgcagatg ttcaaaccgg
     1141 aacgggaagc attgggaagc tgcccccgct gcggttctcc cgtttatgag ggcaagaaga
     1201 attactattg cagcaacaaa gaatgtatct ttaccatgtg gaaaaatgac cgtttctttg
     1261 aggaacgcaa agtagccttt accccgaaga ttgccgctgc actcttaaaa tccggcaaag
     1321 taaatgtgaa aaagctctat tccccaaaga cgggcaaaac ctacgacgga actatcgtat
     1381 tagctgatac gggcgggaaa tacgttaact accgggtgga gcttccgaag aaaaaataac
     1441 tgaatagcaa gcataggaaa agagtaccct tttccgtaaa atccctgctt tctctaccga
     1501 gaacggcggg gatttttgct tgttgggaag tgtcccaaac cctctgtttt gtagagggtt
     1561 ttaccctctg aaatcttgaa aaaatatcta aactctttgg caaaaacgcg ggcgcggggt
     1621 actgaatgat gaaaccggaa catacagcgt ccgcgctgcc gtttggaaag gaggttacac
     1681 atggcaagtt tacgggacac cgtaaaggac tatcaagaag aattacggga cggtatcgct
     1741 tgggtggcgt tttggaaaat gggacgttct tggaacgctg aatatttcca ccttgaaatg
     1801 ggcgactacc tctacccgga ggacagaagc cgcatggaag aaatcaagca ggctgaccct
     1861 gccgccgttg tggtaaacgg ctattattcc ggctatcttg gcgaggatag gaaccttgac
     1921 gaactaaccg ccggggtgcg tcgccactac gaaaacggat atagcaatat cggggaattt
     1981 atcgaagccc acgacgacag gctgccgccg gagcttatcg aggaagcaag agacgccgcc
     2041 cacgctgcgg ggctgccttt ttctgaaaaa gcctaccggg acggcgaaga acccgacccc
     2101 tatctatttg acgggagcat gagcatggag gactacgagc ttatgcaccg catgattgaa
     2161 aacgaaagga gtgaacgcat ggaagaaacg attttaagcg ggtatctttc taatcttggg
     2221 aagtacaccg agggcagacc tgcgggcgaa tgggtatcat tccccacgac tgccgagcat
     2281 ttgaaagaaa tctttgaccg tatcgggata gacggcaaaa actacgggga gctacatatc
     2341 acagaatacc agtcctctat tgcgggactg gcagggaaat tgacagagct tgaaagcctt
     2401 gacgagttga actatttggg cgaacttttg aaaatgcagt ttgacgacga ccgggaaaaa
     2461 tttgtcgcag ctatggcata cggcgaccat acaaggaatt tgcaggacat catcaacctt
     2521 gcacaaaacc ttgattgtta ctggctttat ccgtccgtac agagtgagga agattacggg
     2581 cattatctga ttgaagaact ggacgagttg gagctgcccg aagaagcaaa aaaatatttc
     2641 atgtatgagg aatacgggcg ggacgctgct atcaatgacg ggggcagatt taccgagcag
     2701 ggctatatct acaacaaccg caacaccttt acacagtggt acgacgggcg ggacgtgccg
     2761 gaggaatacc gggtaacgcc gcagccgcca gtacaggaaa aggaacaggc agaccttgac
     2821 gctgctgccg tacagcctgc atttgccaca gagcagcccc cggttctccc gattatccta
     2881 tcttctgaaa agcccgccga taaaatgaaa gagattaccg accgactgga acagggcatt
     2941 ttggggattt atgaaagcga ccgctacgcc gattatctgc gtaccatgtc aaagtttcac
     3001 aattacagct ttaacaatac aatccttatc gccatgcaag gcggcaatct tgtgaaaggc
     3061 tataagcaat gggaaaagga atttgaccgc catgtaaagc ccggagaaaa agctatcaag
     3121 atacttgcgc cgtccccgtt taccgttaag aaacaggtgg aaaaaatcga cccggacacc
     3181 caaaagcctg ttttcgataa agacgggaag cctgttaccg aggaaaagga agtcaagata
     3241 cccgcttttc gtgtggtatc tgtctttgat atttcacaga ccgagggaaa ggagcttcct
     3301 gcccttacct atgagctgac gggtaacgtg gaacaataca aggatttttt cgccgccctt
     3361 gaaaaaactt ccccgtttgt tatgggattt gaaacactaa gcggcggcgt aaaaggacgc
     3421 tgcaattatg aggaaaagcg tatcatcatc aacgagggta tggacgaatt gcagaatatc
     3481 aagaccgcaa tccatgaaat cgcccacgcc acgctgcatg ataccgctct tgccatgccg
     3541 gaacgccccg accgccgcac ccgcgaggta caggcggaaa gcgtcgccta tgctgtctgc
     3601 caacattacg ggcttgatac gtcggactat tcttttgggt atatcgccgg gtggagcagc
     3661 ggaaaagaac ttgcagaact gaaaggctct cttgaaacaa tccgcagcac agccgcgaag
     3721 ctgattgata ccatagacgg gcattttgca gaaatccaaa aggcacagga taaggaacag
     3781 accaccgaac aggcgcagcc cgcacaggaa gccgccaaac agcccgaagc agaagcagcc
     3841 gcgccggagg ttcccgaaga aatagccccg gcacaggaaa aagaagcaca gacagagccg
     3901 gaagctgata caggcgcaag cagcgaagca ccacagcccc cacagccgga acaggctaca
     3961 cccgccgccc cttattatcc tatcaatgaa gctgccgcca aacgtgcaaa ggatatgaac
     4021 agtttttccg attataagca aggcagcgcg acggctgaat acaggcatta tgttgatgaa
     4081 gccgtacagc ttgcagaaag gcagaaacag cgggtagacc cgatgtacca tgataaaatc
     4141 gacagcctgc ttgataccta cgcccggaaa ctggcggcga acatgaacaa aggctatgag
     4201 attgacgcgc gtgttccctc tatcctgatt gcaggcggct ctaactttcc cacaaggaag
     4261 aaagaaaagc agaacgcagc ccgcgacagc aattaccggg aatggcagga catacagggg
     4321 cttcttgata aaatccgcag taccggtatg ggcggtatca gcgcagacga cccgcaggca
     4381 gtacagaagt tagaaaagaa actggaaagc cttgaaaaat cacaggaaac catgaaagcg
     4441 gtaaacgcct attatcgtaa gcacaagacc cttgacggct gcccgcattt gtcgccggaa
     4501 ca
//
LOCUS       DACXMZ010000260         4479 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.261, whole genome shotgun sequence.
ACCESSION   DACXMZ010000260 DACXMZ010000000
VERSION     DACXMZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.261"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..87
                     /locus_tag="JD016_17430"
     CDS             <1..87
                     /locus_tag="JD016_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAU5001398.1"
                     /translation="EYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            87..281
                     /locus_tag="JD016_17435"
     CDS             87..281
                     /locus_tag="JD016_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001399.1"
                     /translation="MEGGDYDASVAHYCLHKFKWKPHEYTDLPDFERAFVAASIDIKV
                     EEEIKEEKKIAKEARRSRRR"
     gene            718..951
                     /locus_tag="JD016_17440"
     CDS             718..951
                     /locus_tag="JD016_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001400.1"
                     /translation="MNKDIELTEKDLYCIARHIQVYVFKKDDEVIKKEDNPCCRCKHK
                     FKCYENSVCIYHCSVFQKLSKITGLKMGFGVKL"
     gene            1011..1538
                     /locus_tag="JD016_17445"
     CDS             1011..1538
                     /locus_tag="JD016_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAU5001401.1"
                     /translation="MREGKRRRGCLFWFIIIIIFSGVVGAIAGNSTNNGSTEKQKEDL
                     TKYIGEEGNIGDLKLTVNSISKASEIPVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDNSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     gene            1680..2240
                     /locus_tag="JD016_17450"
     CDS             1680..2240
                     /locus_tag="JD016_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HAU5001402.1"
                     /translation="MFCSNCGAEITGIGKFCSSCGVAVETEIIEDNNIKSNDLIVDAN
                     GIEINMTEIYRKYRKEKVNAIKNVMEISGLNIKEAKKIVDFSFEELKINFIDDTMSNS
                     EKEKIIQNQNRKNNIEKAQQESVACCPKCGSTSLTAQKKGFGIGKAMVGASLTGGIGL
                     VAGNLGAKKVRVTCLNCGKQFWAGKK"
     gene            2318..>4479
                     /locus_tag="JD016_17455"
     CDS             2318..>4479
                     /locus_tag="JD016_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAU5001403.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMTTAMNIVLSSFEQLQRTSSNAVN
                     ANSIIRAREELARAEAGFDRLERQIRESDNQQRKLNEDINKGASSTDRLVGSAKKLAA
                     TYLGIRTLGGLGNLSDQMTSTNARLGMINDGQQSDAGLNKMIFQSAERSRASYLDTAQ
                     IVSRIGMNAGKAFSSTKEIVGFAEQLNKKFVIAGASTEEMNSALLQLTQGLSSGVLRG
                     EELNAVFESAPNIIQSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAAAEQTNAEFE
                     KMPYTFSQIWTSIKNNAIMIFGVIQKKIEQSMSSKGFRTFIDNFINSLYVLGNVAYNI
                     FNEIISILGSPFFQAFVNAIIVGVSLIVQALGWIITQALNIANVFAQNWSIIAPIVLG
                     VAAAMLVYNNALLLSIANKVKDIALSAKSLVMSFAHIVAESYRAAALVATTIAQDGLN
                     AAMAACPITWILYGIIAIVVAFFVAIAIFNHFAGTSVSAIGVVAGAISVAASFIGNLF
                     IATGNLIIDIVALIYNTLAGFAEFFANFLDDPIGSVIRAVSGMANAVLGIIRSIASAF
                     DTVFGSNLADAVSGWQDKLQGWTDKVAGEAKIKVERMDPNKLHFDRFNYGKAWDAGYK
                     WGDKLETNIKDKFDISKMAEDAKKKLGLDDLWDKKYGLGDGFGSAGLNSPLNDAAKGA
                     KDTAGNTAKMAKTMDKSQEDLKYLRDIAEQEVINRFTGV"
BASE COUNT         1713 a          507 c          911 g         1348 t
ORIGIN      
        1 gaatatacag acctttcaag tgaggtagga gaggtgaatg gttttgatag gacttttgag
       61 gataaagtag aagaagcaaa aaactaattg aaggaggcga ttatgatgct agtgtagctc
      121 attattgcct tcataaattc aaatggaaac cacatgaata tacagattta ccagacttcg
      181 agagggcatt tgttgctgct tctatagata ttaaagtaga agaagaaata aaagaagaaa
      241 aaaagattgc taaagaagct agaagaagta gaagaagata aaatataggt aaaatatgta
      301 agaattatat gttataataa ttgtagcaag aagatgtaat ctacaattta tagagtggag
      361 ttcatactgg gataaaacct acttcctaat gaaaggaggt gggaagtatg gacaattttt
      421 tacttagtat attagctagc ttgatagcta gtttaattgg atatatcgtt tgtagatgta
      481 tcaaaaacgt aaaaagccac tctactcgtg gcaagagtaa aagtggctgg gaacttggtt
      541 ttaaaataaa gttccgcaaa tttaaataat ttatattttt taaaattatg aacttcactc
      601 taccgctaaa tagattgtag ttcttcttgc ctttattata ccacaaatta gaaaaaatat
      661 gcaaagtact tgttttaata gtaagtactt tttttatgtg aaaaagaagg tgattgaatg
      721 aataaagata tagaacttac agaaaaagat ttatattgta ttgctagaca tattcaagtg
      781 tatgtgttta aaaaagatga tgaggtcata aaaaaggaag ataatccatg ttgcagatgc
      841 aagcataaat ttaagtgtta tgagaatagt gtatgtatct atcattgttc agtatttcag
      901 aagttatcta aaattacagg tcttaaaatg ggttttggag tcaaactata atgtaaaaat
      961 tttgaatata tagtataata ttcttataaa atataattta gggggatatt atgagagaag
     1021 gaaagagaag aagaggatgt ctattttggt ttatcattat tattatattt tctggggttg
     1081 ttggagcaat agcagggaac agtactaata atggaagcac tgaaaaacaa aaagaagatt
     1141 taactaaata tattggtgaa gaaggtaata taggagattt aaaattaact gttaatagta
     1201 tttcaaaagc tagtgaaata ccagtagctt ctggttattt ggcgtacact ccagatagtg
     1261 gcaaatatgg tattataaat ttaacaataa aaaatcaaac taaggaaagc caatctttta
     1321 tgctaaattc attcacatta ataggaccag ataattcaaa atatgttcca tctttattga
     1381 ttgatgtagg aaataaatat ataactatgg atactgtaaa tcctaattta gatgtaacag
     1441 gtaatattgc atttgaaatt cctaaggatt tactagtttc tgattgtaaa ctaaaatata
     1501 gtggttcaga gcaagaattt atattaaaag aaaaataata tatagattta aaatcaaaaa
     1561 tactaaaaac acttactaat gtaggtgttt ttttaatgaa aggatgtgat gataatgtaa
     1621 aaattttact aatatagtat aatattctta taaaattatt atactggggg ggaaatgtta
     1681 tgttttgttc aaattgtggt gcagaaatca caggcatagg caagttttgt tcaagttgtg
     1741 gggttgctgt agaaactgaa attattgaag ataataatat taaatcaaat gacttaatcg
     1801 ttgatgctaa tggaatagaa ataaatatga ctgaaattta tagaaaatat agaaaagaaa
     1861 aagtaaacgc aataaaaaat gtaatggaaa taagtggttt gaatataaag gaagcaaaaa
     1921 aaatagtgga tttttctttt gaagaattaa aaattaattt tattgatgat actatgagta
     1981 attcagaaaa ggaaaaaata atacaaaatc aaaatagaaa aaataatatt gaaaaagctc
     2041 aacaagaatc agttgcttgt tgccctaagt gtggttcaac atccttgaca gctcaaaaaa
     2101 aaggttttgg tatagggaaa gcaatggtag gagctagtct aactggtggt ataggtttag
     2161 tagctggaaa tttaggagca aagaaagtta gagttacatg cttgaattgt gggaagcagt
     2221 tttgggcagg caaaaaatag atgtattaat tataaaaaca cttactaatt tagtaagtgt
     2281 ttttattatc taaattaaca gaaaggagag tgaaaaaatg gctacaatac agacatcaat
     2341 tcgcatattt gatggaatga cacctgcatt tcggcatatg acaactgcta tgaatatagt
     2401 actgagttca tttgagcaat tacaaagaac ttctagtaat gcagtaaatg ctaatagcat
     2461 aataagagct agagaagaac tagcaagagc agaagctgga tttgatagat tagaacgaca
     2521 aataagagaa tcagataatc aacagcgaaa acttaatgag gatataaata agggtgcaag
     2581 ttctacagat agattagttg gaagtgcaaa gaagctagca gcaacttatt taggtataag
     2641 aacattagga ggtctaggaa atttaagcga ccagatgaca agtactaatg cgagacttgg
     2701 catgataaat gatgggcaac aatcagatgc tggacttaat aaaatgatat ttcaatcagc
     2761 tgaaaggtca agagcatctt acttagatac tgcacaaata gtgagtcgta taggcatgaa
     2821 cgcaggaaag gcgtttagca gtacaaaaga aattgtaggt tttgcagagc aattaaacaa
     2881 aaaatttgta atagcaggcg caagtactga ggaaatgaac tcggcattgt tacagctaac
     2941 ccaagggttg agttctggcg tattaagagg tgaggaactg aatgctgtgt ttgagtcagc
     3001 acctaacatc atccaatcga ttgcagatta tttggacgtg gacataggaa aaataagagg
     3061 aatggcatca gagggaatgt taacagcaga tattgtaaaa aactcattac ttgcagcagc
     3121 agagcagacc aatgcagagt ttgaaaaaat gccttacaca ttttctcaaa tttggacttc
     3181 aattaaaaat aatgcaatca tgatatttgg tgttatacag aaaaaaattg aacagtctat
     3241 gtctagtaag ggatttcgaa cctttataga taattttata aactctttgt atgtacttgg
     3301 aaatgttgct tataacattt ttaatgaaat tataagtata ttagggagcc cgttttttca
     3361 agcatttgta aatgcgatta ttgtaggtgt tagtttaata gtgcaggcac taggttggat
     3421 aataacacag gcattaaata ttgctaatgt gtttgctcag aactggagta ttattgcacc
     3481 aatagtactt ggagttgcgg ctgctatgtt agtgtataac aatgcgttat tacttagtat
     3541 tgcgaataaa gttaaagata ttgcactatc tgccaaatct ttagtcatga gctttgcaca
     3601 tatagtagca gagtcttata gagcagcagc tttagtagca actacaatag cacaagacgg
     3661 attaaatgca gcaatggcag cttgtcctat tacttggatt ttatatggaa ttatagctat
     3721 agttgttgca ttttttgtag ctatagcaat atttaatcac ttcgcaggca ctagtgtatc
     3781 tgctataggt gtagttgcag gtgcaatatc agttgcagcc tctttcatag gaaacttatt
     3841 tattgcaaca ggaaatttaa ttatagatat tgtagcttta atatacaata ctttagcagg
     3901 ctttgcagag ttctttgcta attttttaga cgacccaatt ggctctgtta taagagcagt
     3961 atccggaatg gctaatgctg tattaggcat tataagaagt atcgcaagtg catttgatac
     4021 cgtgtttggt tcaaacttgg cagatgcagt aagtggatgg caagataaat tgcaaggttg
     4081 gactgataaa gtagcaggag aagctaaaat aaaagttgaa agaatggacc ctaataaact
     4141 gcattttgac agatttaatt atggaaaagc atgggacgca ggatataaat ggggagataa
     4201 gttagaaact aatataaaag ataaatttga tattagcaaa atggcagaag atgcaaagaa
     4261 aaaattagga ttggatgatt tatgggataa aaaatatgga ttaggagatg gatttggttc
     4321 agcaggatta aactctcctc tcaatgatgc agcaaaagga gcaaaagaca cagcaggaaa
     4381 cacagctaaa atggctaaaa caatggataa aagtcaagaa gaccttaaat atcttagaga
     4441 tattgcagag caggaggtaa taaatcgatt tacaggagt
//
LOCUS       DACXMZ010000261         4468 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.262, whole genome shotgun sequence.
ACCESSION   DACXMZ010000261 DACXMZ010000000
VERSION     DACXMZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.262"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     misc_binding    78..312
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            362..1381
                     /gene="pheS"
                     /locus_tag="JD016_17460"
     CDS             362..1381
                     /gene="pheS"
                     /locus_tag="JD016_17460"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAU5001404.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            1398..3791
                     /locus_tag="JD016_17465"
     CDS             1398..3791
                     /locus_tag="JD016_17465"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAU5001405.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVRYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSIIYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            3816..4406
                     /gene="zapA"
                     /locus_tag="JD016_17470"
     CDS             3816..4406
                     /gene="zapA"
                     /locus_tag="JD016_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAU5001406.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         1761 a          518 c          919 g         1270 t
ORIGIN      
        1 tgtattggat atatattaaa aatagtcaca taatttgaat ggaaatgata taatactaaa
       61 ataaacaata taatattgta aatgcaatga aagaggaaag tattttgatt aaacttagta
      121 aagagataaa cacctaggct gggagtgttt tctaagaggt catgagaagt tccctctgga
      181 gtaacagagc tgaaatttta cagtaggctt tgacgtcaaa aacgcgttaa gtttgttaga
      241 ggtggtttga tgatttttaa attgttaaac tactagggtg gtaccgcgaa actatagcct
      301 ttcgtcccta gacagggatt gaggggcttt ttttatacaa aaaaacgaaa gggtgatatg
      361 tgtgcaagaa aaattacttg ctttacgtga agcagctttg gctgaaataa aagaagcaca
      421 aagcatagaa agtgtagaaa gtttaagagt taagtactta ggaaaaaaag gtgagataac
      481 tgccatactt aaagaaatgg gtaaattatc tgctgaagaa agaccagtag ttggtaaggt
      541 tgccaatgag gtaagagaaa acattgaact tagcataaat tctaaaaaag aagaaataaa
      601 tgctattgaa aaagaaagaa aattaaaaga ggaagtgata gatgttactc aaccaggaaa
      661 agttttaaag gttgggaaga agcatccaat aactcaaatt atagatgaag taacagatat
      721 atttatcgga atgggattct ctatagcaga agggccagaa gttgagactg ttgaaaacaa
      781 ctttgacgca ttaaacgctc ctaaagacca tccatcaaga gatatgagcg atacattcta
      841 tatcaatgat ggagtattac ttagaactca aacatctcca gttcaagtaa gaactatgag
      901 aagtcaagag ttaccaataa aagtaattgc accaggtaga tgttttaggt cagactcgcc
      961 agatgctaca cactcaccaa tgttccatca gatagaaggg cttgttgttg gaaaagatgt
     1021 tactatggca gaatttaaag gaactatgga tatcttcgtt gaaaaattgt ttggttctga
     1081 tatcaaaact aagtttagac ctcacaactt cccatttaca gaaccaagtg cagaggttga
     1141 tgttacttgt ttcaaatgtg gtggtaaagg ttgcccaatg tgtaaatatg aaggttggat
     1201 agaaatatta ggttcaggta tggttcatcc aaatgtgctt agaaattgtg gaatagaccc
     1261 agaagtttat agtggatttg catttggagt tggggttgaa agacttgcaa tgcttaaata
     1321 cgaaatagat gatattagat tattattcga aaatgatatg agattcttaa atcaatttta
     1381 attaggaggg tatgtagatg ttagtatctt taaaatggct tagagactat gttgatatag
     1441 acatggatgt aaaagagttc gctgataaaa tgacaatgac aggaactaaa gttgaaacaa
     1501 tagattatta tggtgaagaa atagaaaata tattggttgg aaagatttta gaaataaaac
     1561 aacatccaaa tgctgataag ttggttgtaa ctaaagtaga tattggagat aaagttgttc
     1621 aaatagttac aggagctaca aatatatcag aaggagatta tattccagta gctgtaaatg
     1681 gttctaagtt acctggagga gttgaaatca aacagactga tttcagaggt gaattatcag
     1741 atggtatgat gtgttcagca gctgaactag gtatagatga acattacatt gaggagtata
     1801 aaagaggtgg tatatatatt ttagaccacg aagattctta tgaattagga aaagatataa
     1861 aagatgtttt aggattaaaa gatgctttaa tagattttga attaacttca aacagacctg
     1921 attgtaaatg catgatgggt atagctagag aagcagctgc aactatagga acaaaagtaa
     1981 gatatcctga aatcgaagta aaagaaagtg acgaagagat agatttcaaa gttgagatag
     2041 ataatccaga tttatgtaga agatatgttg ctagaatggt tacagatgta aaaatagaac
     2101 cttctccata ttggatgcaa agaagactta cagaagcagg agtaagacct ataagtaaca
     2161 tagtcgatat aacaaacttc gtaatgttag agcttggtca accacttcat gcttttgata
     2221 taaatcaagt agagactgga agaatagtag taagaaatgc taaagatgga gagaaacttg
     2281 taacattaga tgatgttgag agaacattag acaaagatat gctagttata acaaatggag
     2341 aaaaatcact tggtttagct ggtgtaatgg gtggtgctaa ctcagaaata acttctaata
     2401 cgaagactgt actttttgaa agtgccaatt tcaaaccaga aaacataaga atgacagcta
     2461 aaaaagttgg tattaggtca gaagcatctt caagaaatga aaaagactta gaccctaatc
     2521 ttgcagagat agcagcaaat agagctgcac aacttgttga aatgttagga gcaggaaaag
     2581 ttttaaaagg tgttgtagat gtatatccaa ataaaccaga acctaaaaaa ttggtagtaa
     2641 atcctcaaag aattaaccac ctattaggtg tagatgtacc aatggagcag tttgtaggaa
     2701 ttttagaatc attagagttt aaatgtaatt tggtagctaa tgataaatta gaaatagatg
     2761 taccaagctt tagaacagat atggaacaag aagctgatgt atgggaagaa atagctagaa
     2821 tttatggatt tgagaatata ccttctgtac aattagaagg aaatacgaca gcaggaatta
     2881 aaacttcaaa acaaaaattc atggatgctt taaaagataa ttcaactgct gtaggattaa
     2941 atgaaatatt aacatattca tttgtaagcc ctaagggagt agataagata agagtaccag
     3001 aaggtaatgc aaagagaaat tttgttaaat tacttaaccc attaggagaa gaaacttctg
     3061 taatgagaac tactttaata ccaaatatgt tagatgtttt atcaactaat gtatctcata
     3121 aaatagaaga agtatctgca tttgagtgtg gacatatatt catcccacaa gattcagaat
     3181 tacctaagga agaaaataga atgtgtgtag gtatgtatgg taaagatgtt gacttctttg
     3241 cactaaaagg aactatagaa actatacttg taaatgtcgg atttaaaggt tatgaaattg
     3301 aacctcaaga taataatact accttccacc ctggtagatg tgctaaaata gtgtataata
     3361 ataaatatgt aggtacatta ggtgaattgc atccagatgt tatagaaaac tacaatctag
     3421 gtcaaagagt ttatgtcgct gaaatagata ttgactttgt atttgataat tctgatagaa
     3481 caaagaatta tgtgccatta ccaaaatatc catctacatc aagagatata gctcttattg
     3541 ttaaagatga tgtatttgta aaacaaatag aagatataat aaaggaaaat ggtcaaggat
     3601 tagtagaaag ctacaagtta tttgatgttt acaaaggttc tcaaatagaa gctggataca
     3661 agtcaattgc atattcaata atttatagaa gtaaagataa aactctaaca gatgaagatg
     3721 tagctaaggt gcatgataag atattaagtg aattaagcga aaaattaaat gctaacttaa
     3781 gatcaaatta acaactaatg tcgggggagg ttaggatgaa caaagtaatg gttaaaatcc
     3841 atggcgctga atatccaatg gttggagaca aatcagagaa gtttatgatt agtattgctg
     3901 attttgtaga caaagaaatg gataaaataa cacgtcaaaa cccgaaattg agcttatcag
     3961 ttgctgcgat attaactgca ttaaatatat cagacctttt atttgaatgt tcagatgaga
     4021 atgagaagtt gattaaagcg aatgaagaat tgagtaagaa ggttggagca tctaatgaag
     4081 agcttcaatt agaaatcaaa tctttaaagc ttacgatagc agaaaaagaa gcagaaaatc
     4141 gtgaagctga aactaagatg aaagagttaa ttgagattat agaaaataaa aagcaagaaa
     4201 tattcgaact tagtaatacg acagaaggtt ctagagcaga gttagatgct tataaaaaca
     4261 agattgagga gttaagtact caacttgaag aagcaaatga aagagctact atagctgaaa
     4321 acttagcttc tgagtttcaa aacaaagctt atgatttaca gttaaaatgt acagggttaa
     4381 ataatgatgc aaaaaatgtg gaataaacta aaagatttgc tgtctcttgt tttttgagac
     4441 agcttctttt aatgtataaa tataaatg
//
LOCUS       DACXMZ010000262         4465 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.263, whole genome shotgun sequence.
ACCESSION   DACXMZ010000262 DACXMZ010000000
VERSION     DACXMZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.263"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(36..581)
                     /locus_tag="JD016_17475"
     CDS             complement(36..581)
                     /locus_tag="JD016_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HAU5001407.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(699..2522)
                     /locus_tag="JD016_17480"
     CDS             complement(699..2522)
                     /locus_tag="JD016_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001408.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KSGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMCEGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(2535..4310)
                     /locus_tag="JD016_17485"
     CDS             complement(2535..4310)
                     /locus_tag="JD016_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001409.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1446 a          795 c          393 g         1831 t
ORIGIN      
        1 agtgagatac tcctaaaatc tactttatat taaatttatt ttccaaaata tctatttaat
       61 aatcctaaaa atgctttacc atgtctagct tcatctttac acatttcatg tactgtatca
      121 tgtatagcat ctaatcctaa ttcttttgct ttcttagcta attttaattt tccatcagtt
      181 gcaccatact cagcatcaac tctaactctt aagttttctt ttgtatctgc aactacaact
      241 tctccaagaa gttctgcaaa tttagcagca tgttcagctt cttcaagagc tattctctta
      301 taagcttctc ctacttctgg ataaccttct ctatcagctt gtctactcat tgctaaatac
      361 attcctactt ctgtacactc accaacaaag ttagctctta atccttcgat tatttcttca
      421 tctacacctt gagctactcc tattctgtgc tcatcagccc aatttttttc gcctttcatt
      481 tcttcaaatt tatcagctcc aactttacat actggacatt gtgctggtgc agcatctcct
      541 tcatgtatat atccacatac tgtacaaaca aatttcttca taattacttt cctcctaaat
      601 tttatatatc aaattaaatt ttttatttat taagttagtc tatatatacc cgcaccatat
      661 acctttaaac aattttttta aaagtttttt ctaaatactt attttccctg tgtcaataat
      721 atgtttctat aataatgtcc ctttaatttt atcaattctt tatgatttcc tgattccaca
      781 attctaccat tttctaaaac tattatatta tcacaatttg atatagtact taatctatga
      841 gctataatta tacttgtctt tccataagtt aaattattta ttgcttcttg aattttagct
      901 tctgttgcta tatctatgtt acttgtagcc tcatccaaaa taagtattgg agcatttttt
      961 aatactgctc tagcaatact tattaattgt ttttgacctt cacttaaatc ttcaccccta
     1021 tccgataata cagtactata tccttcacac attttaacta taaatttatg tgcatttgct
     1081 attttactag catatataac ttcttcatca gtagcctcca gatttccata ccttatattt
     1141 tccataactg tatcattgaa tatataacct tcttgtggaa ctactccaaa acaagttctc
     1201 aaatagtttc tgttaataga gtatatatcc ttaccatcta aaaatatact tccttcatct
     1261 atatgataaa aatttgtaat tagatttata atagttgttt ttccacttcc cgtctctcct
     1321 atgatggcat ttgatgtgcc agatttgatt tttaggttta tattatctaa aattttcttt
     1381 tttgaatcat aagaaaaact tacatcttta agctcaattt ctccctttac aaaatgagag
     1441 tttaaattac catctttatt atcgttaatc tcacttttaa acatgttatg cacttcttgc
     1501 tcttcttgtt catctataat ttcaaaaact ctttcacatc cagctatggc tgaaagtaat
     1561 gtattaaata gatttgctaa ttcatttaaa ggtcttgtga attgtcttac ataacttaaa
     1621 aaactagcta taacccctat ggttataata tctttagatg caagaatgcc tcctataata
     1681 catatagttg caaatcctat attactaatc acattcataa taggcattaa aagagaagtg
     1741 taaatttgcg atttaaggga aatatttaga agtctatcat tgacttcttt aaaatcattt
     1801 acagattttc tttcataatt aaaagatttt actacattta tgtttgatat catttcttca
     1861 atatgtgcat ttagttttcc caactcaact tgttgttgct taaaaaaaac tctagttctc
     1921 tttgcaataa aattactcac aaacattact atcggtatta atattacact gcaaaaggtt
     1981 aaaattgggc ttaatacaaa catcataata aaagtcatca atatgtttaa gattcctgta
     2041 attaactgta ctaatgaatt tgatattcct gtactgatat tatcgacatc atttgtcact
     2101 ctactcataa tatctccagt agaactttta tcaaaaaagg ttattggtag atttttaaat
     2161 tttaaaaata aattacttct catttctctg actatacttt gagataatct agctgtacat
     2221 atccccttag acagtttaac tgaaaactca attatataaa atgaaagtaa aataattatt
     2281 ccttttaaga aaatggattt tgaataagta tcgatatcta ttatgtcaac tacctttcca
     2341 atagtatatg gtattacagt tgatataatt gaatcaatta ttactagcaa aaaaataaat
     2401 ctagtaatct tcttatcttt tttaaagtac ataaaaattc ttttaaacgt ttttttaaaa
     2461 tctttaggct tttctttttt ctttaatcta ctgaccttct gatttccagt acttaaattc
     2521 aatatacttc ctccttaatt tacatcttca cttactaaat tctgcaacgc aaaaattttt
     2581 ttataaattg gagaactaac cagtaaatct tcatgtgtag caaatccaac cacctcaccc
     2641 tcatccaaga ctaaaatctt atcacagtca attatagaac taatcttttg tgaaatagtt
     2701 attacagtac atttcttact ttccttttct cttaaattac ttatatactc ttttatattc
     2761 tttcttataa ttgtctctgt attaatatcc attgcactaa aacaatcatc aagaattaat
     2821 atttgtggtt ttttggctat agctctagct atagtaattc tttgtctctg tcctcctgat
     2881 aaatttttac ccctttggaa taaaacttca tcataagtat tttcgagttt tgttataaat
     2941 tgatttgcat ttgctatttc acatgatttt acaacatcct ctttgcatat atctctattt
     3001 cccataacta cattctccaa tatacttttt ccaaacacac tactattttg aaatactaca
     3061 cctattcttg cccttaaact ttcttcagaa atactgtata tactacgtcc tccaatctta
     3121 atatcacctt tatcaattat atataatttt tgaattaaat ttactaaaga agtctttcct
     3181 gaaccagttt gaccaatcac accaatattt tgaccttctt ctattttaaa ggatacgttt
     3241 tttaatattt tttcatctgt atttttgtta tacgaaaaat ctacattttc aaaaattata
     3301 tcacagttta caaactttaa atctttttct ccaacatcta aactttcttc tctctcaatt
     3361 acttcttcat tctcatcaac caaaacctca cttattctct cataagatgc ttttgcccta
     3421 acaaaaacat taaaaacatg tgaaatcatt ataagagatg ttaatattct agtcatatag
     3481 tttacataag caactataat cccaatttta atttctcctg aataaattaa atgccttcca
     3541 attagtaata ttattattat ggaaacatta atgattattg taattgtcgg tctaaaaaat
     3601 gacatcatag ttgtagcttt tctactattt ttaaaaagtt catcatttaa ctcttcaaag
     3661 ctttggttct cataatcgta tctacaaaat agtttaacta cttttattcc acttaaaaac
     3721 tgtcttaatt ttgaatttac tttatctgta ctttcttgta catttttaaa taatctatac
     3781 cctatcttca tattaagcaa tattataata aatataatta aaactgcaat tagaataatt
     3841 attgacatct ttaaatttaa ttttatagct ataattaaac ttcctataca cattattggt
     3901 gactttataa aaactctcat cattccatta acgaattgtt gcatttgatt gatatcatta
     3961 gtaattcttg ttattaatga agctctacca aatttatcta tttgttttaa tgagtatgta
     4021 tttatctttt caaatacatc ctgtcttaaa tcataagaaa aatgctgaga aactcttgcc
     4081 gagattacac ttcttattat tgcaaatatt gcacctaaaa aagttatacc tatcattata
     4141 gctcctgttg atagtatgta gtctaagttc ttatttgaaa cccctatatc tattattttt
     4201 gccattatag ttggttgagc taaatcacaa aaagcttcca atgttaaaaa aaatactgct
     4261 gttagaaata atactatata tttttttatg tattttttaa aatacctcat tcaatcatcc
     4321 cttcatagaa ttttctcatt accaaaagta tatcataatg gctaatttaa ttgattatat
     4381 caattataaa tatttgattt attattaatt tttataccat atattttata aattatttgt
     4441 ttttatttcc tatatttata tatat
//
LOCUS       DACXMZ010000263         4456 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.264, whole genome shotgun sequence.
ACCESSION   DACXMZ010000263 DACXMZ010000000
VERSION     DACXMZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.264"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(476..1633)
                     /locus_tag="JD016_17490"
     CDS             complement(476..1633)
                     /locus_tag="JD016_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HAU5001410.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            1787..2356
                     /locus_tag="JD016_17495"
     CDS             1787..2356
                     /locus_tag="JD016_17495"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAU5001411.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            complement(2538..2954)
                     /locus_tag="JD016_17500"
     CDS             complement(2538..2954)
                     /locus_tag="JD016_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001412.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(3180..4274)
                     /locus_tag="JD016_17505"
     CDS             complement(3180..4274)
                     /locus_tag="JD016_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001413.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
BASE COUNT         1553 a          770 c          481 g         1652 t
ORIGIN      
        1 aagataatga acatttatat tataacctat ttttatatcg ataagtatga aattgtttaa
       61 ccaagtcaaa atgatatata aatttatatt taaatgtgtt tagagtttaa tttattttca
      121 atgtttaaaa aagttattct atgcatctaa tttaattagt aaactgaggt tctagttaga
      181 taaattatat tataatgaat atataatatg cagtttgcga tattttaaaa gtaaatgata
      241 taatatttgg aacaactggg ttgcaatgtg tttttaaaaa atatcaaaat aaggctatga
      301 tttttaaaac catagcccta tctgtaaaaa tttaattatc acttaaaaaa taaattttta
      361 tttaaagaat acacataaat atttccagac tccctcatga actaaaaagt tcattttgag
      421 acatctctct tataatgttc tttattttat agtataagtt tataaaaatt aaatcctatt
      481 tatcaatctg ttctctaagc cacttagttg ctacatctaa ctgtttattt tcatctttct
      541 cttcaatagt aggctttata cctttttcat tgatataatc cccattaggt gtaaaatatt
      601 gagcagtagt tagcttgtat cctgttccat ccttcaatct tacaactgat tgtaccaacc
      661 ctttaccaaa agttgtagtc cctacagaaa taccttcttt attatcaacg attgcagctg
      721 ttaatatttc agcagcagaa gcactttctc cattagttaa cactactatt ggaagtccca
      781 atttttcttt atcagatttc aggtactcag tatttccctt attatctttt gtatatacta
      841 tggttccttc accaattaac tcatcagcaa cttctttaca tatatctagc attccaccag
      901 gattatttct aaggtctatt actaatccct tgatattttt tgcttttaat tcttttaaag
      961 ctttatcaaa gtcagtatat gtacttgata taaattgggt aatttctata tatcctaaat
     1021 cattatcaat tactttactt tctatactct tctcaattat ctggtctgtt tttactttaa
     1081 agttcaattg tttatcttct cttaatatag tcagttcaac tgttttacct tttttaccct
     1141 tcatcatacg aacagcttca tctgaatttt tatatgagac agattttcca ttgactttta
     1201 ctaatttatc ccctgatttt acacctgctt tctctgctgg agaatctttc atgggaacta
     1261 ctactaagcc accatctttt gatgccccta tatacatacc tacaccaaca taagaacctt
     1321 cactatcatt aattagattc tccatttcat ctttagtata ataaccagaa tacttatcat
     1381 ttgttcctag aaatagacct tttgctgctc catttaccaa atcagtatct gatacttttt
     1441 tataaaaatc ttgttttata atagattcta gtcctaacaa cttatcatat tttttatagt
     1501 cttcatagag ttctttcgat attacaactt tattccccaa tgtcaactga aatgctgatg
     1561 taaccatagc tgttataatt actaaaatca cccctaaaaa aatagctttc ttctttgaaa
     1621 tcacaagata catctccttt tacataaact attattatta tatgactagc tgttattttt
     1681 attacattta cccaaatata ttgctttatt acaataaatt atactaaaat ttatattaaa
     1741 tacaattgct gtacacatta taacatatta agggaggaat tattttatga aaaaagaatt
     1801 tagaaaaaaa gttattgaat caagaaaaaa taagagtgca aacttcgtac ttagtaattc
     1861 aaatttaatc actgaaaaat tacttcaaat ggaagatata agaaatgcaa ctaatataat
     1921 gctttatcta aattttaaca atgaagttca aacagataat ttaatcaaaa aacttttgtc
     1981 tctaaagaaa atagtatcaa gtcccatcac aataaaagaa aatcatacat taatccctac
     2041 tcaaataaca gatttaaaag agggattgag aattggagca tatggaataa gagaacctaa
     2101 cgaaaaatca cctacaattg atattaaggc cttagatgtt gtaattgtac cagctgtagc
     2161 atatgatata cattgctata gattaggata tggtggagga ttttatgata gatttttaga
     2221 aaacttaaga aaagatgcta taactatcgg catagcattt gacttccaga tattcgattc
     2281 tattccaaaa gaaactcatg atgcacaatt agactacata gttactgaaa ctagaattat
     2341 cactccaaat agttagaata ttaagtattt ccagacccca tttaatgtag taataaatac
     2401 tactgttaat atcttacttt ataagcattt taagtcatgg cttttacaac aataagaacc
     2461 atatacaatt tacttacatt aataaaaacc atgactttat tgtacaatat gcagttattt
     2521 aattataaaa taccttctta tttcgtcgca tttaattcta ttctatctgg aagttcactc
     2581 atatttgttt caacaactat gtatggaaaa gaatcttcct tatctttata ctttatcttg
     2641 actctcaaaa catcctttcc ttcctcaacc acactttcta ttttgtccat ttctatacca
     2701 tatttattat ttcctctagt cactataatg tatattttgt tatcgatttt agccgctaat
     2761 gctctttctt catttgtata cttatccatc acatctaata tcttttctgg tattgaattt
     2821 ctttgtaata ctgtataatc tatgggttta gatttctcta caaacatact tggtatgaat
     2881 ataaatcccc ctataacgaa tacaagagct ataactccat aaattatacc tttaatatta
     2941 aactttacat tcaatcttat cactcctccc atatattcta aatatatgat ttataaactt
     3001 atcttagact ctaaattatt gattagtata tatttataca taattataaa tatatgtaat
     3061 gtaactaaaa aacatgtttt ttctatttca taataaacga gctaaaaaat aaaaagagct
     3121 atatgaaaac aatgaaaatg ttttcatata gctcttgagt ttacaatatt aaaaaatact
     3181 tatttgtcac tgattccttc ttgatatcca gatgtttttg taatctgtct ataaagaaca
     3241 caaaccagta ttatgacgcc catatatcca gtatatggat ataaaatacc aaccagctta
     3301 tcaaatggta ggaagccacc tataagacct aatatagctg ctattaaagt tattaattta
     3361 aacttaggat gtttatcatc tacaaatcta ttagttactg accataataa aggtacagct
     3421 gttgtatata tacctaatat aagaattatt gagaataaaa ctccaattac tggtgaaatc
     3481 ttatctgcta agaataatga tggtattgct tttgaatata agtttcctat atcagaaaga
     3541 atagctaagt gcatcattat agctgcagcc ataagtgcta ctccacctaa tataccacca
     3601 caaatagctt cttttttgtt agcagcactt gctcctaatc cagttaagaa tgccataaca
     3661 acaattgtgt tataagtagt atatattaca ccagatagat aggcatttgg aactggttta
     3721 gctatcttta cagtgctaag aacttgtgca gcttctccta tattacctat gtttttagct
     3781 aaacttatac caccaactaa aagtgtaaag attattatta taggccctat tccacctagt
     3841 atctttgata atttatcaag acctagtgaa acagttaata atgctactac tgccatacct
     3901 actctaccta caaatggatt taatccatag tattcagtta aagttgctcc tgctcctgaa
     3961 atcattacta cgaaaacacc aaataagaat aaaggtccaa accattcaaa gaatattcct
     4021 aaatacttac cacaataaac acgataaatt ttgataggtt ctttaagttg aagctcttta
     4081 ccttttgaca taactgaagc acccatccat gagaataata ccattgaaat tatcccacca
     4141 ataatacctg caaaaccata aaaagagaag aattgcatta tttcctgtcc tgttgcaaat
     4201 ccagaaccga ttactgttgc tacatacgca cctgcgaaac tgatgactgt tttaacattt
     4261 actttttctg acatatctca gttacctccc catgtgaaat tttttcttct gattaaacaa
     4321 ttcgataaac tacctacaat ttcctttaac ataaaataca tgataactgt tttatagctt
     4381 tttagtataa taacttaaaa cttataatct gattttatac cctactcctc ctctcataat
     4441 ttttttagat tctttt
//
LOCUS       DACXMZ010000264         4388 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.265, whole genome shotgun sequence.
ACCESSION   DACXMZ010000264 DACXMZ010000000
VERSION     DACXMZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.265"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            231..1310
                     /locus_tag="JD016_17510"
     CDS             231..1310
                     /locus_tag="JD016_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001414.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDNINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="JD016_17515"
     CDS             1634..2002
                     /locus_tag="JD016_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HAU5001415.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVEKVLEFGNLVITVTHVYAINKSLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2110..2916
                     /locus_tag="JD016_17520"
     CDS             2110..2916
                     /locus_tag="JD016_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAU5001416.1"
                     /translation="MSTMEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLF
                     DFQIQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDRQNMKKVVLIGQSA
                     GGFVAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNI
                     ISKMCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDK
                     DKTGKVREYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQL"
     gene            3308..4198
                     /locus_tag="JD016_17525"
     CDS             3308..4198
                     /locus_tag="JD016_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAU5001417.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKEIIKAYKRSESYTQIKFF"
BASE COUNT         1676 a          475 c          828 g         1409 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttgtgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaacagt aggaaatatc
      121 gtatgagtga gaatatcgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagtatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacaa cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggtt gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacccg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtacgg taaaaataaa tctactaact
      901 ggattgtaaa gcatggttgt agaactctac taaaaaaagg caatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat taaatagtta tacaatagcc atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaagatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaaag gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaatca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaagt agcgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatactttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgagcactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccag atacagtact tttcaaaaaa tcataatgta
     2281 ttagtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gatagacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggat taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaattgttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgatgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagagaatat aatgagcagt ggcataataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttataatata taaaaattac atactcatta
     2941 ggtaaattat gtagtatatg tgtttataga tggttataga aataaatatg taataaacta
     3001 cttaacactt aaaaagtgga tttgtccaga gcataataca aagcatgata tataaatttt
     3061 taagcgaaat acatcaaaag aagtattaga attagtggag ataaaacagc tattagagat
     3121 tataaataaa tagttttaaa ggtactacga atatagagga aggtaaatca tacgttattc
     3181 aaggattata aatgattagt ttaaaagtac tatcggatag gagtctccat atttaaacct
     3241 tagtaaaggt agttaaagta gtagtttaaa aactaaaaaa ttatataaag gaattaggta
     3301 taagaaaatg attgagagta taccagcaaa acaaatttta caaaaagtga aatttgacaa
     3361 tacaagatgg tttggaattg actataatat gaatctctat cgtggttgtt ctcatggatg
     3421 tatatattgt gatagccgta gcacaatata caacatagag aattttgaca aagttagata
     3481 taaagaaaat gtgatagaaa ttttaagtaa agaattgcgt tcaaaaagaa aaaaaggggt
     3541 agtaggtatt ggagctatgt ctgacacata taatcctttt gaaaagcaac tttgtattac
     3601 aaagcaagca ctagatttga tttcggaaaa tcattttgga gtgtctattg atactaaaag
     3661 tagtttggtg gttagagata taccgatttt gcagaaaatt aaaaaaaata attctgctat
     3721 tgtaaagttg actataacca ctgcaaatga tgaattatca aagaaaatag aaccttatgt
     3781 aaatccaagt tctgtacgat ttgaggcagt gaaagaactt aatgattctg gaattttttg
     3841 tggcatatta ttaactccta tgcttccatt tttgactgat aataaagatg aaatacgagc
     3901 aattgtagaa aaagcacaca aggctaatgc aaaatttatt tattgtatgt atggtgtaac
     3961 aatgcgtagt ggtcagagag agttttttta tgagcattta agagatatat caccaaaatt
     4021 ggtacttaaa tatcagaaaa catatggttt aaactatgta tgtactgtac aaaataaaga
     4081 ttgttgtgag aagttgttaa gagaagaatg tgcacgttat ggtattttga cggatatgaa
     4141 agagattata aaagcgtaca agaggtcgga gtcatatact caaataaaat ttttttaaga
     4201 taggctttgg atgagatata atagtggtga ttgatgtgtt gatattatag ttgattgtta
     4261 gagaggatat tatagatgaa tattagtatt aatattttag gtaattgcta gagctgatat
     4321 tatagatgaa tattaatatt gatattctag gtaattgcta gagctgatat tatagatgaa
     4381 tattaata
//
LOCUS       DACXMZ010000265         4372 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.266, whole genome shotgun sequence.
ACCESSION   DACXMZ010000265 DACXMZ010000000
VERSION     DACXMZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.266"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            330..2429
                     /gene="nrdE"
                     /locus_tag="JD016_17530"
     CDS             330..2429
                     /gene="nrdE"
                     /locus_tag="JD016_17530"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAU5001418.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGNKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDKWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2442..3413
                     /gene="nrdF"
                     /locus_tag="JD016_17535"
     CDS             2442..3413
                     /gene="nrdF"
                     /locus_tag="JD016_17535"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAU5001419.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLENEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3489..4115
                     /locus_tag="JD016_17540"
     CDS             3489..4115
                     /locus_tag="JD016_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001420.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDTRKVSPESLKTVLRFEVEREV"
BASE COUNT         1757 a          457 c          793 g         1365 t
ORIGIN      
        1 agctatttag agataattta aatttttaaa caggacataa taagcttgta ttgttttata
       61 tttgagttga taaaaatttc tttaaataaa aataatgtca attataaagt aataaaagtt
      121 tagatataaa aaaatttgtg tatatactat atgtagtata taatagataa agcactgact
      181 atatgtagtg cttttgtttt tgaaaaatac ttattgcatt tctataatta tagattaatt
      241 ttgggatttc aactgataag taccaaaaga aataataagt aaattaagtg aaattatggc
      301 acaagattat gttaaaagga cggtaaataa tgaattggat agagcttaac aatcaagtga
      361 taattaaaaa tgaacaaggc aagtaccaat tggaaaagga taaggaagcc ttaagtagtt
      421 acatagaaga atttatacag ccaaagttga gaaaatttaa taacctagaa gaaagattga
      481 agtatttaat agatgaaggt tattattcaa aagaagtaat aaatcagtat agcatagatg
      541 ttattaaaaa tatatatgat atgattgata aacataagtt tgaatttcaa tcatatatga
      601 gtgcaaataa gttttatcaa aattatgcat taaagagcaa tgatggaaag gaaatattag
      661 aaacatataa tgataaagtg ttaattgtag ctttgacact aggaaatggc aataaagatt
      721 tagctttaaa cttggcagat aaaatagtaa aacaagaatt tcaaccagct acacctactt
      781 ttttaaatgc tggaagaaaa agggctggag aaatggtatc atgtttttta ctatctgtag
      841 aagatagtac agaaggtata tcgtatgcta tatcttcatc aaatcactta tcaaagattg
      901 gtggaggagt tgcactaaat ttatctaaac ttagagcatc tggcgaatct atcaaagata
      961 tagaaggtgc agctggaggt gttgttggag tagctaaaat gttagaacaa tcatttagtt
     1021 actttaatca aatgggagct agacaaggtt ctggtgcggt gtaccttact gtattccatc
     1081 ctgattttga attgttgatg gatactaaaa agataaatgc agatgaaaaa ataaggcttg
     1141 ctacattatc attaggagct ataataccag ataaatttat ggagttggca gaaaaaaatg
     1201 aagtagcata tgcattttat cctcatactg tgtataagaa atatggagtt tctcttgatg
     1261 agatagaaat ggataagtgg tatgataaat tggttaatga tagtgatata agaaaaaaag
     1321 aaataaatcc taggcagatg ttgacaaaaa tagcacagat gcaacaagaa agtggatatc
     1381 catatgtagt gtacatagat acagctaata gagaacatac attaaaagat gttggaatta
     1441 ttaaaatgtc taacttatgt tgtgagatat ttcaatatca aacaccttca gaaatagagg
     1501 ggtatggagg aaaaaatgaa tggggacaag atataagttg taacctaggt tctttaaaca
     1561 tagccaatgt aatggataat aaaactatag aaagtacagt tgaaacagca ataagagcac
     1621 tgtcatttgt agcagatagc acagatataa aaccagtgcc aacggtaagc aatagtaaca
     1681 gcaagtctca ttctatagga ttaggagcta tgaatttaca tggatactta gtaagagaaa
     1741 atattttata tacttcagat gatgctatag atttttctaa tgtatttttt gcaatggtta
     1801 gatactattc aattaaagct tctatgaaaa tagctataga aagaaatcaa acttttgaag
     1861 gatttgataa atcagaatat gttaagggaa gaaatagcaa ggttttatca aaatactatg
     1921 aacaatctta tctacctaag tcagaaaagg taagagcatt atttgaaggg atttatatac
     1981 caacaaaaga agactggaca aaattattgg atgaggtaaa agaaaaaggg atatataatg
     2041 catatttaat ggcagtagca ccaactcaaa gcattagtta tgttcaaaat gctacatcaa
     2101 gtataatgcc aattactgag ccagtagaag taagaacata tggagactct actacaatat
     2161 atccaatgcc atttttaaca aatgataata tgttgtatta tcaatcagca tacaggatgg
     2221 atatgagaaa ggttatagat ttagtggcaa cagttcaaaa ccatgttgac caaggtatat
     2281 caacaacact gtttgttaca gatgaaaaga caactagaga tatagctaga cattacatat
     2341 atgcctataa aaaaggtttg aaaagtttat attacacaag aacaaagatg acaagagata
     2401 ctcatgagtg tctagtgtgt tcagtatagg gagagaaata tatgacattt aatttacata
     2461 aaatacataa tgcagttaac tggaatagag aagaagatgg atttacacaa gctttttggg
     2521 aacaaaatgt aaaacaattt tggcttcctg aagaaatatc tgtttctaaa gacataaagg
     2581 tatggaatga actaagtaat aaagaaaagg aattatataa aaaagtattg ggtggtttaa
     2641 cattactgga tacaaaacag gggaataatg gaataccatc tatgatgagt ttaacagaaa
     2701 acctacaaag aaaagctgtc ttgtctttta tggggactat ggaagaaata catgcaaaaa
     2761 gttattcttc tatatttatg actctacttt ctaatttaga aatagatgaa ttatttgaat
     2821 ggatagaaac agaaccaaca ttacaaagaa aggctgattt agttttagct caatacgaaa
     2881 atactacaaa tcaagaggga ttatacctat caatggttac aagtgtattt ttagagagtt
     2941 tcttgtttta ttcaggattc ttttatccat tgtatctatc tggtcaagga aagatggtag
     3001 ctagtggaga aataatatcg cttattctta gagatgagtc tttacatggt aagtatatag
     3061 gattgctagc acaagaaata tacgattcat ttgataaaat ggataaggag atgttagaag
     3121 agaagatgta ttctatactt tatagcctta tggagaatga gatagagtat acaaatgtta
     3181 tatacagaga aagtgggctt gaaaatgagg ttgtgaactt tttaaaatac aatgcgaata
     3241 gagctttaga aaatctaggg tttgagaagc tatatactgt agatgctata aatccaattg
     3301 tattaaatgg tcttagcact gaaaccaaaa cacatgattt tttttcaact aagggtaatg
     3361 gataccaaaa aggagtgtat gaggaattag aggatgaaga ttttataata taatttataa
     3421 ttatgacaca ctgttgtcac ttttagcgtg ataatatcaa tgtataaaat atatatggag
     3481 gtatttttat gaaatatgaa tggagaaaaa gagataaaga gttatattta cctaaaaaga
     3541 atcctgtaat agttgaaata ccagaacaga aatttatcat gttaaaaggg agtggagacc
     3601 caaattctaa agaatttact gaagcaatag gagtacttta ttctttggca tatgcaataa
     3661 aaacaatgcc taaaaaagga attgtaccag aagggtattt tgactatacg gtttttcctt
     3721 tagaaggtat atgggataag ggagaaaaaa gtaaaggctt agatgtactt attaaagaag
     3781 atttaattta cactataatg atacgtcagc cagattttgt tacacaagac ctgttggata
     3841 aagctactga tatagtaaag aagaaaaaac ctcataatct tcttgaaaaa gttagatttg
     3901 ggtctattaa tgaagggcta tgtgttcaaa tgcttcatat aggttcatat gataatgaac
     3961 cagaaagctt tgacattatg aacgaatttt gcaaaaaaaa taaacttata agaaaatctt
     4021 acacacatcg tgaaatttat ataagtgata ctagaaaagt atcgcctgaa tcattgaaga
     4081 cggttttacg ttttgaagta gaaagggaag tataaatatt ttgaataatt tataagagtt
     4141 ataaaaagtg aagtctgttg agacttcatt ttttgatgca attttaaaat taaatttgat
     4201 gtttcataat attttaaaaa ctttctactt ttattagtgt gaataactgt tgctattaaa
     4261 taataatact aattatataa gtattattat ataatattta tagattatta tataaataga
     4321 ttattagtaa gatgtatgca ttattaatta aaataaatta catatactat tt
//
LOCUS       DACXMZ010000266         4352 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.267, whole genome shotgun sequence.
ACCESSION   DACXMZ010000266 DACXMZ010000000
VERSION     DACXMZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.267"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            112..1587
                     /locus_tag="JD016_17545"
     CDS             112..1587
                     /locus_tag="JD016_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAU5001421.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1674..2087
                     /gene="speD"
                     /locus_tag="JD016_17550"
     CDS             1674..2087
                     /gene="speD"
                     /locus_tag="JD016_17550"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAU5001422.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2117..2968
                     /gene="speE"
                     /locus_tag="JD016_17555"
     CDS             2117..2968
                     /gene="speE"
                     /locus_tag="JD016_17555"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAU5001423.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            2958..3836
                     /gene="speB"
                     /locus_tag="JD016_17560"
     CDS             2958..3836
                     /gene="speB"
                     /locus_tag="JD016_17560"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAU5001424.1"
                     /translation="MKNNFYHRNTFMSMNKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDAAVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4016..4216)
                     /locus_tag="JD016_17565"
     CDS             complement(4016..4216)
                     /locus_tag="JD016_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAU5001425.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1717 a          494 c          748 g         1393 t
ORIGIN      
        1 tgttaatata ataaatatca tgagtttaat aaaagtaaaa ataaaaaata gaaataataa
       61 ataataaata ttaaaaatat ttgaatatag agaggagatg aatttgaaat catggatagc
      121 gagtttgcaa atgcagttga attaagctat gagtgtgctc cattattaga gtcgttaaag
      181 gaatactcag agaaagatat agcttgtttt gatgtgcctg gacatgtaaa aaatagaggt
      241 gtagctatat taaataaata tctaggtgaa agtctcatga aaatggatat aaattcatca
      301 ccaactatgg ataatgtatc tgcgccaaat gggattataa aaaatgcaca agatttgcta
      361 gcacaagcat atatggctga tgaggcattc tttataacga atggcacaac tcaagcaata
      421 catgcaatga ttttgagtgt tataaaacca ggtgaaaaag tattacttcc aagaaatata
      481 cataaatcag taataaatgc gttaatactt tgtggaggaa taccaatatt tatacaacca
      541 gaatttgatg aaaaactagg tataagttta aatattactc ttgaaaaagt tagaacagag
      601 atagaaaaag attgtaatat aaaagcatta tttttcctta atccaactta ctatggagtt
      661 tgtgcagacc ttgagagtat tatagaacta tgccataaaa ataatgtttt agtgttagtt
      721 gatgaagctc atggtgcaca ttttccattt catttagatt tacctccttc tgcaataagt
      781 ttgggagctg acatggtagc tgtaagtata cataaaactg gaggagcatt gacacaatct
      841 tcagcactct tattgaatag agataatgta agttttgaaa aggttcttca atcaatcaat
      901 atgctacaat caacatcagc atcttatttg cttatggcaa gtatagatgg tgccagagtt
      961 aatctagttg agaacggtga aaagcaactg tcaaaagcat taaatctatc taggtatgca
     1021 aaatctaaat taaacaaaat tgatggaata aaagttttat ctacagaaat attgaaacaa
     1081 aaaggtgtta aatttataga tgaaacaaaa ttgtgtataa atgtaaaaga attaaatttg
     1141 actggatttg aagtatacga tttattatat aaaaattttt caattcaagt agaattggga
     1201 gattcatata atatactggc acttgtgtcc attggaacaa ataaatcaga tatagataga
     1261 ttagtaaaag cactttctat aattgcaaag atttatagaa aagaatcaac attaaatgaa
     1321 tttaatatgg ttcaaataaa tccaattatt aaacttaatc caagagaagc tttttatgca
     1381 ccaaaggaaa gtgtagaaat caattcatgt atagatagaa tttgtggaga atcaataatg
     1441 gcatatccac caggtatacc gattattgct ccaggagaat tgataacaga agaaattatg
     1501 gagtatatta ttttcttaaa aaatagcaat gcatatctta cagatgtaca agataaaaac
     1561 ttagatagaa tattagtaat aaaataaatt actttatttt gcaagggcaa gtatttgtta
     1621 agtagagttt atcaaataga acttattaaa agtataaagg agttggggtt gaaatgaaat
     1681 ttgaaacact aggaagacat atactagtag aatactataa ttgtgatgaa aacgtactta
     1741 aagacccacg tcttatagaa aattttatga atgattcagc attaaatgca aaagcgacaa
     1801 ttgtggattc tgtattccac cattttaatc cttggggggt ttcaggagct gttataattg
     1861 ctgaatctca tttgacaata catacatggc ctgaatatgg ttatgcagca gcggattttt
     1921 ttacttgtgg tgatatagac ccatggaaaa gttttgaatt attagagcaa cttttaaagt
     1981 ctgaaagaag cgagtctaca gaaattccaa gaggcttaac tacaaaaatt aaaaaatatt
     2041 caaaaaaaga tttaggtaag attactcata aaccagaagc agtataaaac atacaagaga
     2101 aagtgaggat tattatatgg aactttggta tacagaagaa tggacagaaa atgtaagatt
     2161 ttctataaaa gtaaataaac atctatttga aggaaagtct caatttcaaa ggatagatgt
     2221 atttgatagt gatgaatttg gaaaatttct aacaatagat ggattaatga tggtaacata
     2281 taaagatgaa tttatatacc atgagatgat aactcatgtt cctatggcta caaatttaaa
     2341 cataaagaaa gtattagtca taggtggagg tgatggaggt acagtaagag aattaagccg
     2401 ttatcctcaa atagaaaaga ttgacatggt tgaaatagat aaaatggttg tagatgtatc
     2461 taaggagtat atggatatat gctcatgtaa attagatgat aaaagagtga gtttatattt
     2521 tgaagatggt gttaattttg ttaagtgtgc acatgataaa tcatatgacc tgataatagt
     2581 agattcaact gacccaatag gaccaggcga agggcttttc tcaactgatt tttataagga
     2641 ttgttataga atactgactg atgatggaat tttagtaaat caaagtgaaa gtccgtattt
     2701 tgactttaat gctaaagaga tgaaaagagc taataaaaag ttaaaacaga tatttccaat
     2761 atcagaagtt tatcaagcac atataccaac atatccatct ggtcactggt tgtttggatt
     2821 tgcttcaaaa aaacttaatc cagtaaaaaa tcaagataga aatggatggg agaaattaag
     2881 tttaaaaact aaatattata atagtgatat acatttaggt tcgtttatgt tacctcaata
     2941 cgtgaaggag atgctagatg aagaataatt tctatcatag gaacactttt atgagtatga
     3001 acaaaaatta tgaagaatct aatcttatag tatttggtgt tggatttgat ggaactactt
     3061 ctaacagacc tggggctaga tttgcaagta gctctatgag aaaagaattt tacggtcttg
     3121 agacatatag tcctttttta gatttggatt tagaggatta taatatatgt gattatggag
     3181 atttagaaat tagtgttgga agtacagaac aagtcttaaa agaaatctat caagagacat
     3241 ataagattgt tagagattca aaggtaccct ttatgattgg aggagagcat ttagttacat
     3301 taccagcctt taaagcagta catgaaaagt acaatgatat atatgtaatt cattttgatg
     3361 cccatactga cttgagggaa gaatataata atagtaaaaa ttctcatgca acagtaatta
     3421 aaagaatatg ggatattgta ggtgataata aaatatttca atttggtata aggtctggga
     3481 caaaagaaga atttaaattt gctacagaag aaaaacacac atacatggaa ataggaggaa
     3541 tagatacgtt tgaaaatata gttaatatgc taaatggaaa gaatatttat ctaactatag
     3601 atttagatgt attggatgca gctgtgtttc caggaacagg tacaccagaa cctggtggtg
     3661 taaattatag agagtttcaa gagattttta agattataaa aaactctaat ataaatatag
     3721 ttggttgtga cattgtagag ttaagccctg attacgatac aacaggtgta tcgacagtta
     3781 tagcttgtaa aatcttaaga gagttatgct taataatatc tgataaaatt aaatagagta
     3841 ttttatataa atagtatata ttataatata agaagttatt ttaaaaaagt tagtttaagt
     3901 ttgacgcttt tctaaagtaa cttcttattt ttgtaattaa aaataaatat aataaacaac
     3961 aaaaaagagc cctcgatagc tccttttcta aaatatatat atttaaatta aataattata
     4021 ttctatttac gttttcagct tgaggtcctc tattaccttg agttatatca aagctaacct
     4081 tttcgccttc ttctaaagtt ttgtatccat cattttgtat agctgagaaa tgtacgaaaa
     4141 catcatcttc tccctctaca gatatgaacc caaatccttt ttcattgtta aaccatttta
     4201 ctattccgtt tttcattata agaaaacctc cgcattatta aaaaatacaa actaaaaaaa
     4261 taagtctgtt ctattaatat aacataaatt ttaaataaaa gcaaataaaa tttttaaaaa
     4321 aattaattac ttttagaatt taataaaaat tg
//
LOCUS       DACXMZ010000267         4297 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.268, whole genome shotgun sequence.
ACCESSION   DACXMZ010000267 DACXMZ010000000
VERSION     DACXMZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.268"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(462..680)
                     /locus_tag="JD016_17570"
     CDS             complement(462..680)
                     /locus_tag="JD016_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5001426.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(681..1088)
                     /locus_tag="JD016_17575"
     CDS             complement(681..1088)
                     /locus_tag="JD016_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001427.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(1174..1662)
                     /locus_tag="JD016_17580"
     CDS             complement(1174..1662)
                     /locus_tag="JD016_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HAU5001428.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     IAVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(1867..2988)
                     /locus_tag="JD016_17585"
     CDS             complement(1867..2988)
                     /locus_tag="JD016_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAU5001429.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIEKGIAKGKR
                     EEKFNIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(3361..3531)
                     /locus_tag="JD016_17590"
     CDS             complement(3361..3531)
                     /locus_tag="JD016_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001430.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(3703..4146)
                     /locus_tag="JD016_17595"
     CDS             complement(3703..4146)
                     /locus_tag="JD016_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU5001431.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         1451 a          687 c          389 g         1770 t
ORIGIN      
        1 tatttggaag taatttcata tttatttaac ttaatttttt tgtgataaaa taattctaat
       61 tttcatatag atattattat aaattttcta gtttatggag tcattcattc tataattcac
      121 aaatatgtat tttttaatct attaaagtat tcattttctg gcttataagc atggacttct
      181 tatctataag cttggttttt catctaataa gttatttatt ctttaatcta tgagcttgat
      241 ttttcatcta taagtcattt attttttaat ctatgagttt ggtttttcat ctataaatca
      301 ttcatttttt aatctataag cttggttttt acctataatt aattcattct ctaatctata
      361 agcttggttt ttcatctata agtcatttat tctctaatct ataagcttgg ttttttacct
      421 ataattaatt cattctctaa tgcataagta atacatgaaa tttaaatatt attttcttca
      481 ataaattcaa acaactcttc tacagttgta tcaaatacct tagcaatatc cattgccagt
      541 tttaaagaag gattgtagcg accattttct aaatgtacaa tagtttcacg tcttactcct
      601 actgactttg caagttcacc ttgtgtcata aggttctttt ctctatagga tttaattttt
      661 gtctttagta atactgacac ctaattgtat atcccctttc tgtcaaaata tataaataga
      721 attaacttca acaaggattg aataaacaaa atgactaata aagacattcc tatatttaaa
      781 tttgacacat ttatgtccaa agttacaaga aatatagata ctatagctac agaaaaaaac
      841 aatactttta ttgcaatatc attaactctt gatagtatac attttgatgc ctcatcaatt
      901 aaatcatcat actttttatt atagacccac tgagctccca tagctaatac ccaaataatt
      961 ataggaagat attcaatact acttccttta aaaaaatact tatttactaa tgtagctcca
     1021 atacaataag aaattcccaa tatttctgaa ataagtgttg atttaatacc agtttgttgt
     1081 ttagacatat aaaatccccc ttaaaaagtt atgtatttat aacacctctt gttataaata
     1141 tataactttt ttgaataaat ttcaataaaa atattatttt gcaacaaatt cttttattaa
     1201 ttttacatta gatagaggca ctttagatgt cactccttgc aatgtcaaat cttctatatt
     1261 aacttcattt aattttgaac acagttctaa gtcaaatatt ctttcccaac ttaccttctt
     1321 ttcaatttgt tcatcttcat aaagctctac agcaatatcc attaaaggta aatgccataa
     1381 atcaaattct gataataaca cattgtcttc acttatttct atttcaagta aaacaccttt
     1441 ttttcctttt tctaaataac cactacatct taaatcaggt ctcttcgtcc aaacccatat
     1501 catactatca tcaaattcat atattctatt tgtcatttga ctatacatcc atttgtatgg
     1561 aatatcaaag tcttctaata taaaagaata atcatttgat agatatttat tgtttttatt
     1621 gttattccaa aattctaaag tctgtatagt ataaaatttc atatataaat caaccccttt
     1681 gtttttctat aagaaggaga tggttcaaac ttttattttg aattatctcc tttatttata
     1741 cacctctttg tacctattat ttatatatct ctttatatct accatattta aataacaaag
     1801 ttttatatta atttaaattt aaaagaggaa gctttgtatt tatctagtgc catctcaatc
     1861 atagtattag ttttgattat agactttttc aacctctgat ttattcaaat catctataat
     1921 atcgcatatt tcactcaaat tgtatccttt attttttaat tttatgatga tattaaattt
     1981 ctcttctctt ttcccttttg caataccttt ttcgatacct ttttcgatac ctttttcaat
     2041 accttcttga aaaatttctt ctttaaaatt tctactttta ttcataatat acgcacctct
     2101 ctttaaattt aataaatagt cctcattact acctaattct tttaatctat caagtgctct
     2161 tttaataaca taatccgttt tagatagtat tctaatatct atatgagaag gattatcaat
     2221 aagtttaccc catattagaa ctctgtttgc tatacaactg gatttacgaa tttttggaag
     2281 ttctataaaa tataatgcag ttttactaga aaaaatatct ggatgcccgt ccattgttaa
     2341 tctcaggcaa tttacataat cttctatgtg attatatttt acatgattta gtatatgtat
     2401 tccaacagtt tttccaattt ctttatattc tttaccttct tctaactttc taccatgtac
     2461 acgactaaga taatattcag ctctttccaa ataatcctct tcaagagctc tttgcatctc
     2521 aatatttata tggttacgtt cttcatcttc agccaaaata tcaagtctag gtcttttctc
     2581 accaatgtaa ttaattgttg ggtcggtttc aaccttaatt attcttttta tttgagtgaa
     2641 ctgtttgata ttcctattag catatggagc atctttgagg atatcattta taaaatttgc
     2701 aagtaaatcg taatcattat ttaaagtaaa caatgattta aatacctcat catactcagg
     2761 cttagcatac ccatcttcag ttacaacttc tccaacattt tcactagtca atatttcttt
     2821 tggaattaaa tccttgattt tatctaattt ttcattagaa aaatcacctc tactaagtat
     2881 gtcatttaat tttttggtcc attctctgtc cttttcttca attatagttc gtttatactc
     2941 ttttgatgtt ttaataatct tagatttttc ttctaatttt tctttcatat caacacctct
     3001 tttttattat tacccatttc ttcataccaa agtctgaact tctaagtaaa atttgcatta
     3061 ttgaaataag aaccttataa acttatatat cattactaaa gttaagactt taaagagaag
     3121 cacttagatt acaatagatt ttagatagct ttctttttaa aactcttgtt gataaatgaa
     3181 tgaatataag ggtatgtatt aaaatagaga tactcatctt tttcattgtt taagtaatga
     3241 aaatatattt tatttaatac aaaaaagagt gccttataaa ctaaaagttc acttcgagac
     3301 actcacttac gttttgtttc ttaaacttca caatattaca tacttattta ttaatttaat
     3361 ctatgcatac ttaacccaat aataaagtac atatatccaa ctcattaagc catgaaaaat
     3421 agcccatgga atagatttcc atgcagtata agaaataatc attgctaaac aagaaccaaa
     3481 agaaataccc tttttaacag ttgattttat agtattatta tttgaatcca taattaatcc
     3541 tccaaaattc aaaataaaca agttattata tatattattt gaatataata caatgtaaca
     3601 gcttctaaat aaattatact cttattatac ataatttata aattataatt ccgataatta
     3661 tatgaattaa ttaattatat taacctaaaa atattatcct atctaaaagt tatatgatag
     3721 atacaacatg tcaacacatt gtaattctcc ttcaaagatt ggttctggat aattatctac
     3781 aaaaaagttt ttaattttat gtgaataaac aaatccaaac ttctcataaa aaggaactcc
     3841 gctttcagat gtaccaacaa ccattgatga atatttttta ctataaacat ctaaaagatg
     3901 ataaatcatc tttgaggcat aacccatatt ttgaaaatgt tcatatgtag caatgttttt
     3961 taattcacat atttctttgg aaatttcatt tacaactgct atacaagtaa gttctttatt
     4021 atcatacaat gcgtacatag ttcctttatt gagatattta tcaatcattt cttcacatgg
     4081 atcggctaac atcaaaaggt caatttgact ttttttattt atttctatag tttttatttc
     4141 tatcataagt tctccaccct cactttattc aaaccttatt ttagcaaact tcttacagta
     4201 cttatttagt tcattttgat atatattttt acttttttaa aatatagtga taattcccta
     4261 gaatattaat actaataata aattataata tcagctc
//
LOCUS       DACXMZ010000268         4148 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.269, whole genome shotgun sequence.
ACCESSION   DACXMZ010000268 DACXMZ010000000
VERSION     DACXMZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.269"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            374..1648
                     /locus_tag="JD016_17600"
     CDS             374..1648
                     /locus_tag="JD016_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAU5001432.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPCLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYADIPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYVKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1855..2172)
                     /gene="trxA"
                     /locus_tag="JD016_17605"
     CDS             complement(1855..2172)
                     /gene="trxA"
                     /locus_tag="JD016_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAU5001433.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2439..3191
                     /locus_tag="JD016_17610"
     CDS             2439..3191
                     /locus_tag="JD016_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAU5001434.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3178..4059
                     /locus_tag="JD016_17615"
     CDS             3178..4059
                     /locus_tag="JD016_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAU5001435.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
BASE COUNT         1621 a          539 c          573 g         1415 t
ORIGIN      
        1 tatattttcc attatgttct aatcaatttc tttactattt ttacaacttt attagtcata
       61 ttcataatta tttctaatta gtgtttaaca ttttatgtaa aatttataaa aaaatgtata
      121 taaattaaaa taagtgacta tactaaaaat atgatacgga taaacaataa gttcaaccta
      181 tctcccccaa aatcaagtga gctacagttt cccccaactg tagctcattt tcgtgtaatt
      241 taataaacat ctcaacaaga attaattaac gaaacccctt ataaaaacat aacatatatt
      301 aaacaagctc ttgattaaaa cttgagcttt tttaataaaa aacagaatag tttattagat
      361 aaggggtttt tttatgcaat tcatggatgt atctaaatac attgaaggtg caaatcttaa
      421 agttaaaact aaaaatggtg aaactgttaa aagaatatat tttaacaact ctgccactcc
      481 attagtttta aaaaacgtag tggataactt aaactgtgag attccttgcc ttacttatat
      541 taatgctcca ggaataatct cagaaaaaaa tactttaaaa tatgaaaatg tacgctctac
      601 tatcctcaaa cttattgatg gagacgaaga aaaggattct gttatttatg tcaaaaatgc
      661 tacagaagga ataaacttct tagcaaaatt ttttaaagat gaaaatccag ataaatttgt
      721 tattaccaca gcaatggagc atatggctaa ttacctgcct tttaaagtta attttcctac
      781 aaaagttgtt ggaattacag aaaaaggaga acttgattta tgtcaattag agaatatact
      841 taaaaattat gctggtaatg tatctctcgt tacagttaca ggagctagta atgtcacagg
      901 tataaccact cctatatatg aaatagcaag aatagctcat aagtatggtg caaagatatt
      961 ggtggatatt gtgcaagtaa ttcagcataa acctttttca atgatgccac atgaatgtga
     1021 tgagcatata gattttgttg tttttagtgc tcataaatgt tattctccac taaatggagg
     1081 agctctggta ggacctaaaa aattctttga aaaatttaat cctgtactat atggctcagg
     1141 ttctaccaaa tttgtaaatg ataacaaaat actatatgca gacatacccc aacgttttga
     1201 agcaggatac ccagagtatt ttggaactct tgcaatggga aaagcactga acactttaaa
     1261 taaaattggt ctaaacagaa tttcaaaata cgaaagagaa ttatttttat atgttaaaga
     1321 agaaatgagt aaaataccta atataataat atatggagat aaatcaaata atgtaattat
     1381 cccaaatata tcttttaata taagtaatat gtattacaaa gatgtagcaa aatatttagt
     1441 tgataacttt ggaatagaaa ctggagctgg agtagtaggt gcagatattt atgtacaaaa
     1501 attattaggt ataagtccaa aagaagcata tgtcagattt atgagggaaa atcctgtagg
     1561 tcttgttagg ataagcttag gaatgtacaa tacttttgat gaggtggata gctttataca
     1621 tgctattaaa actttggctt ttaaataagt tttttctatt cttttttatc aataaataga
     1681 gagtagtttt gttttcacaa aaaagcaaat tatatcaaca gtcaatttca ttagcaatta
     1741 gacactcatt tgtaactata caaactattt tacactatac aaacaaaaaa ctcattacaa
     1801 tgagtttttt tgtttatata tgagatttat tttaattact tcaatttact ttaattacat
     1861 atatgattca attttgtttt ttaaattctc cttaggcata aaacctatta atctatctac
     1921 aggttttcca tccttaaata ttagcatagt tggtactgta gatacttcaa atttctgagc
     1981 taattctata ctttggtcta tgtccacctt caagaagttt gctttttcaa ccatctcatt
     2041 tcctaaagct tcatatattg gtgatagcat cttacaaggt ccacaccaag tcgcaaagaa
     2101 atctacaact actaaaccat ctttattttc tacttcattt ataaattcat tattatttat
     2161 tatttttacc ataatataca tctcctttat tatcttcttg atattaatat actacgccct
     2221 atataacagt tcaattaggc acttttttgt aaccgcctat ttttaatagg tttaatatta
     2281 tgataaatag atttataaga ttaaatattt atgagactaa atatttctaa tatattttat
     2341 ttttttctta atatagttga cattttttcc cttaaattat aaaatcaaat taataactac
     2401 ataggtagtt actaatcttt tatcaaagga gtttctatat ggataatata attaatgaat
     2461 tacaaaatat aggatttact aaatacgaat ctcaaatata catatcatta ttaaaggaaa
     2521 gccctttaac aggatatgaa ataagtaaat tatcaggtgt accacaatca aaagtctatg
     2581 aaaatataac aaagctatta aacaataata taattatcaa tgttggaact gaccctatta
     2641 agtacatacc aataaaccct aatgaattat taaaaaataa gaaaaaagaa tttgacaatt
     2701 ctatggataa tctaaagaat agcttacaga tattaaataa aagtaaaagt gtagaatacg
     2761 tattaaacat aaaaggagat aaaaatatat taaaaaaagc aatagaaatg ataaaaaatg
     2821 cgaaacaaga aattataatt tcttgccctt atgaagaaat tcttgaatta aaaaaagaac
     2881 tcctaattgc ttacaaaaaa aatattaata ttgaaatact acttttagaa aacaaaagtc
     2941 tgaaaggtct tgataatatt catattcatg gaggtaataa aactacactt gaaaataaaa
     3001 tgctttacaa aggaattatt ttgattgtag ataatgatga aatattgaca ggaaacttat
     3061 cagaaggcag tgaggccatt tcaatttgga caaaaaactc taacatttta tatattggag
     3121 ttcaatacat aaaacatgaa atttatattt cagaacaaat aaaaggaggt tataaaaatg
     3181 aacaaaatta agattaaaca agttgatgct tttacaacta taccttttgg tggaaatcct
     3241 gctggtgtcg ttactgatgc ctctaacatt tctgatgaaa ttaagcagaa tgtggctaaa
     3301 gaaatgaatt tatcagaaac tgcatttgtt tcagagtcaa atattgcaga ttttaaagta
     3361 caatttttca caccaggttt agaagttgat ttatgtggtc atgcaacaat aggtactttc
     3421 catgcacttt tggaagaagg gaagcttaat actaataaga gtatatttta tcaagaaaca
     3481 aaagctggtg tattatctgt agagcttaag aaaataaata atgaaaatat atttatgatg
     3541 gaacagaata cacctgaatt tgaaaattta gataattgca gaaaagaact tgcacaaatg
     3601 ataggtgtgc atgaagatag acttcttgaa tatcctatta taaaggtaaa tactggactt
     3661 tggtggcttg tatttggtct aaaatcttta gatgatttaa aaaatatctc tccagattta
     3721 aataaaatca aaaaatttag tggtgataat aatataattg gtataactcc attttgtata
     3781 gaaactatag ataaaaagtg tgactaccat ttaagatcta tagcaccata tgttggagtt
     3841 acagaagacc ctgtatgtgg aactgggaac gcttgcgtat catcttatat agtacatcac
     3901 aatattttag ataaaactag ttttattggt gagcaaggta actttataaa cagacctgga
     3961 aaggtatatg taaatataac tagagaaaac aatgacataa cttctgtaaa aattggagga
     4021 agtgcttata ctgttttaag tggtgaaatt atgtattaat cccataccta ataaaaacaa
     4081 ataaaaatac tagctaacta aagctagtat ttttatttgt tatttttatt taataatcat
     4141 actctaac
//
LOCUS       DACXMZ010000269         4094 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.270, whole genome shotgun sequence.
ACCESSION   DACXMZ010000269 DACXMZ010000000
VERSION     DACXMZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.270"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            60..2414
                     /locus_tag="JD016_17620"
     CDS             60..2414
                     /locus_tag="JD016_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAU5001436.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEKSLKNTSNKVNYASDELDMALHKVNYVSDELD
                     TTSNKVNYVSDELDMALHKVNCVSDELDMTSNKVNCVSDELDMTSNKVNYVSDELDMT
                     SNKVNYVSDELDITSDKTDYISNELYNLSNKSITDNIPIKMAVITKSNMLNQLPDLVE
                     LERVFSKIEFKVCFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVK
                     PAHKLMDEYYFFMELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFT
                     IHNPVAWEDKKFETPSGKYELYSESIKNLGISPTPVYISNKYKGIEDKNISFRLLTNH
                     HADTLFSQHFMDKKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGN
                     YIVKMYVGWWKKHGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            2502..2837
                     /gene="spoIIAA"
                     /locus_tag="JD016_17625"
     CDS             2502..2837
                     /gene="spoIIAA"
                     /locus_tag="JD016_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HAU5001437.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            2851..3279
                     /locus_tag="JD016_17630"
     CDS             2851..3279
                     /locus_tag="JD016_17630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HAU5001438.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3302..4066
                     /gene="sigF"
                     /locus_tag="JD016_17635"
     CDS             3302..4066
                     /gene="sigF"
                     /locus_tag="JD016_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HAU5001439.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
BASE COUNT         1677 a          397 c          786 g         1234 t
ORIGIN      
        1 aaattatagt atatatgtta atttttaata aggtaagaaa gtagaaagaa ggatatttta
       61 tggatataat aaaaaaatta agtcatgggt gtacattaga ttgtcatgac tgttgtaaat
      121 tcaatgttta tactaaagga aataatgtag taaaaattga aggagataaa aatcatcctt
      181 atacaaaagg ttttatatgc aaaaaaggga tggcccattt agatagatta aatcataaag
      241 atagaataaa gactcctatg cttaaagttg atggagtatg gaaagagatt tcttttgata
      301 aagctataga aataatggca gaaaagctta catactataa agaaaagtat acatcaaagt
      361 ctgtcatgca ttatgaccaa tatggaagtg ggtctgtgct aaaatacata ggagatatat
      421 tttttaattt ttatggaggt gttagcagac ataagggggg tccctgttgg agtgcaggga
      481 tgcatgctca aaaatatgat tttggagtag ctaagagtca tgctatagaa gatatgttaa
      541 atagtaaaag tatatttgtt tggggaaaaa atccagcata tactacaatt catactatgc
      601 aaataattaa aaaagctaaa gaaaaaggga taaagatagt agtgattgac cccatatata
      661 caaaaacagc tcaaatagca gataagtatg tacaagtaaa tcctggaaca gatggagctt
      721 tagctatagc tatggcaaaa attatagtag aagataaatt atatgatgaa gaatatataa
      781 actcgtatgt tattggtttt gaagaatata aaaaatattt atcttcattg gaattaagtt
      841 ttttaattga tgaatgtggt gttaaagaga atgatataag agagttagtg gatttatata
      901 ctaataaata ttcaagtata aatgttggat atgggcttca aaaatataaa aatggtggaa
      961 acacaataag agcaatagat gctttaggtg ctataactgg tcaaatagga tttagtggtg
     1021 gaggggtaaa ctatgctaat aaagtttatc cgagtgttat aaattctgac ccttacaata
     1081 gccaatcata tggtgaagat agggagtttt atgtaagtaa tatcagtaaa tttatagaaa
     1141 aatctttaaa aaatacatca aataaagtaa attatgcatc agatgaactt gatatggcat
     1201 tacataaagt aaattatgtg tcagatgaac ttgatacaac atcaaataaa gtaaattatg
     1261 tatcagatga acttgatatg gcattacata aagtaaattg tgtatcagat gaacttgata
     1321 tgacatcaaa taaagtaaat tgtgtatcag atgaacttga tatgacatca aataaagtaa
     1381 attatgtatc agatgaactt gatatgacat caaataaagt aaattatgta tcagatgaac
     1441 ttgatataac atcagataaa acagattata tatcaaatga attgtataat ttatctaata
     1501 agtcaatcac agataatatt cctataaaga tggctgtgat aactaaaagt aatatgctga
     1561 atcaactgcc agatttagta gaattggaga gggtgttttc aaaaattgaa tttaaagtat
     1621 gttttgatat gtttataact gatacagcta ctttatgtga catgtttata ccgtgtacaa
     1681 atacactgga gagtgaagat ataatttata gctcaatgac caatccatat ataacctata
     1741 atgaaagagc tgtaaagcca gcacataaat tgatggatga atattacttc tttatggagc
     1801 tagctaaaaa gatgggatta aatgattatc cttttgtaga aaagagaact tatttagaaa
     1861 aagtgattga accacttaag cgttttgaca aaaatctgga tatagaaaaa cttaaaaaca
     1921 attactttac tatacataac cctgttgctt gggaagataa gaagtttgag actccatctg
     1981 gaaaatacga gctttattca gaaagtataa aaaacttggg aataagtcca acacctgtat
     2041 atataagcaa taaatataaa ggaatagaag ataaaaatat ttcttttaga ttattaacta
     2101 atcatcatgc agatacttta tttagtcagc attttatgga caaaaaatct atagctcaag
     2161 catatataaa tcggagaatg gctaaaaaag taggtattga ggacaaagat attgtgatat
     2221 tgaggtcaaa aaaagcaaaa ataaatgtac aaataaatat agatgatgga gtaggtaatt
     2281 atatagtaaa aatgtatgtt ggttggtgga agaaacatgg aaatcctaat agtttaacag
     2341 acactggtat atcagatttt gggggtcaag taacatataa tgaaagtatg gtagaaataa
     2401 tacgacaaaa ctagttaaat ttcattgaag gaataaaaat ataattatag aattgattaa
     2461 aagttactat ttttacaaaa ctattgaagg agggattttt gatggtaaat tattccttag
     2521 aacataagaa tctatacata gaatttatgt gctcagaact agaccaccat gtagccaatg
     2581 aaataaggga agaaatagat aacttattaa gtgtaaatca agtaaaaaat gttgtattta
     2641 attttgaaaa tattaatttt atggattctt caggtatagg cgtcatcata ggtagatata
     2701 aaaagatttc taatgaaggg ggacgagttt cagtaattaa tataagttca cgagttaaga
     2761 agatttttga tttatctggg ttaaataaaa ttattggcat ttatgatacg tatgaagaag
     2821 ctctaagttc tttgtaggga ggagtatgtg atgaataata ttatggaagt taaattttcg
     2881 gcaatatctg aaaatgaaag tttagcaaga gttatagtag catcctttgc agctaagtta
     2941 gacccaacag tagatgagtt agtagatata aaaactgctg tctctgaggc tgttacgaat
     3001 gctataatac atggatatga agaagacagt tctaagtttg ttttcttaag atgtgagata
     3061 gaaggaaata ctataaagat tatagttgaa gatgaagggt atggaataga aaatgtggag
     3121 aaggcaatgg aaccacttta tacatcaaaa ccagaacttg atagatcagg aatgggattc
     3181 acagtaatga aaagctttat ggacgatgta gaggtaagtt ctgtaaaaga taatgggact
     3241 agaattgaaa tgactaaaaa aataaatgtg cctaaataaa gtccaaaagt gaggttttat
     3301 tatggaagta actgttgcca gagaagaaaa aaaacctctt cttagtcatg aagagactct
     3361 agaacttatt gaaaaagttc aaaatggaga tgaagaggca aaagaaatat taatatcaag
     3421 caatttaggt ctagttagga gcgttgtatc tagattttta aatataggat atgatagaga
     3481 agatttattt cagttaggtt ctataggctt aataaaatca atatataaat ttgacccaaa
     3541 atttaatgta aaattctcta cttatgcagt accaatgatt ttaggtgaaa taaagagata
     3601 cttaagagat gatggtatga taaaagtatc aaggtcgtta aagcaaatag ctgtaaaggc
     3661 aaagatggaa agtgaagctt taactaaaaa attaggtaga gagccgagta tagaagaatt
     3721 agctaaagca atagatgtag aaaaagaaga tttagtaatg gctatggaag ctaatttcaa
     3781 tgttgagtat ttgcaaggag ttattcatga agaggaaggt tctccaatat gccttattga
     3841 taaaataagc atgaaaggag aaagtgaaga agaaaaagta gtggataata tattgttaaa
     3901 agacatactt ggaagactgg ataagagaga gcgtcaaata atagttctca gatactttga
     3961 agacatgact caaagtgaaa taggagagat gctcaatata tcacaagttc aagtatctag
     4021 gatagagaag aaagtattgt ctaagttaaa agaatatata tcatgataat acttatgata
     4081 tatagaataa caaa
//
LOCUS       DACXMZ010000270         4047 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.271, whole genome shotgun sequence.
ACCESSION   DACXMZ010000270 DACXMZ010000000
VERSION     DACXMZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.271"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            253..657
                     /locus_tag="JD016_17640"
     CDS             253..657
                     /locus_tag="JD016_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="HAU5001440.1"
                     /translation="MDKTLKKDLNQEYTESKSLIMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            674..1270
                     /locus_tag="JD016_17645"
     CDS             674..1270
                     /locus_tag="JD016_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001441.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            1307..3901
                     /gene="clpB"
                     /locus_tag="JD016_17650"
     CDS             1307..3901
                     /gene="clpB"
                     /locus_tag="JD016_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAU5001442.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
BASE COUNT         1705 a          393 c          717 g         1232 t
ORIGIN      
        1 taatgtattt tatgaaagtc tgccatatta aatagattta aattttttta aaaaatttat
       61 aaatattgtt caaaaatcct gccaataaat tggaaatact aaattaaaaa aataataaag
      121 tttagcttat agctaaataa aagtttactt ataaggtgaa ctttttgcat tttcagtttt
      181 gacatacata tttttttgta atatatttat attatcaatt agagataagg agagtgagaa
      241 gagatattta atttggataa gacattaaaa aaggatttaa atcaggaata tacagagagc
      301 aaaagtttaa ttatgaataa aagtaagtat atgaatgttt gttttaagaa ttatattaaa
      361 gtagtagaag gaatgtatac taagagggat ttaattccat taaaattaat aaactctatg
      421 gataaaaata ataagattag aaaaacatta tatgaattat cttgtgaatt tgaatatcca
      481 aaaacttctt tatcttcatt gtttacggag ctgaaaaaaa tggatttttt acaaagagtg
      541 aaaaatggtg agtatatgat aaatccacat atagcatata aaggtagtag agctgataaa
      601 gaaagtttat tagcagaata taatcagata aaaagaccta ataaggttag taaataagga
      661 agaaggtttt tatatgagtt taaaacttga tagaaatgtt ctacagtggt ttgactatgt
      721 atttgagaat gaaaaaacta gtttgagaca ctataatttt aattgtacac taaaggagat
      781 tagttctacc agtttgaaca aagtagcttt tattttagaa aaaaataact ctaagtactg
      841 gaagctttat tttgaaatac cagctgaagt tactttaaaa cttaagcaaa acattcatcc
      901 tttatttagg gaatatatat acgaacaaat atcactgtat aacaataatc aaatttacaa
      961 ttttgtaaat tcaaacatat taaaagtgtt taataatatt gctatttatc aatataatat
     1021 tttagaaaat ctatacacta ttgattttaa aaagagtttt atagataaat gtcagtattt
     1081 attaataggt gaaaaaagac ttatagatga agacttgtat ttaatagcaa aatcaaaaga
     1141 agtattcgat ttttttaatt cagatggaac atttaattta acattgagtt tcgatataca
     1201 aaaaaacgag aatttacttg atagtctttt agagcttaga aaatcaataa ttataaatga
     1261 gagaatttaa tttatttgga tataatatta aaatggaggg atttatatgg acgtagaaaa
     1321 aatgacttta agagttcaaa aaagtttaaa tgaggcttat aacgaagcag taaaaaatca
     1381 taaccaacaa gtagatgtaa ttcatctttt ctcagcactt attaatcaag aggatggatt
     1441 gataccaaat ataatcgaaa aaatgaatat atcaattgat tctttgagaa atactgtaaa
     1501 ctttgaaatt gataagcttc caaaagtata tggagaaggt gctgactcac aaggagtatc
     1561 agctactaga aaaataaatg aggtgcttat aaaggcagag agtatttcta aagaatttaa
     1621 agattcatat ataagtgtag agcatgttat gcttgcaatg atggaaacag aatcaaaatc
     1681 agctgttggt aaaatattaa aacaatataa tataaataag aatgactttt taaatatttt
     1741 atcacaagtg agaggtagtc aaagagtaga aacacaagac ccagaaggca catatgatgc
     1801 acttgctaga tatggaacaa acttagtaga tttagccaag aaaaataaat tagaccctgt
     1861 aattgggaga gatgaagaaa taagaaggat tataagaatt ttatcaagaa gaacaaaaaa
     1921 taatcctgta ttgattggtg agccaggtgt aggtaaaact gcaattgtag aagggttagc
     1981 agagagaata gtaagaggag acgtcccaga aggtttaaaa gataagattg tattctcact
     2041 tgatatggga gctttaattg ctggtgcaaa atatagaggt gaatttgaag aaagattaaa
     2101 ggcagtttta aaagaagttc aaagttctga tggaaaaatt atactgttta tagatgagat
     2161 tcatactatt gttggagctg gtaaaacaga aggttcaatg gatgcaggta atcttattaa
     2221 acctatgctt gcaagaggtg aattgaactg tattggtgca actacttttg atgaatatag
     2281 aaagtatata gaaaaagata aggctcttga aagacgtttt cagcctgtaa ttgcagaaga
     2341 acctactgtt gaagatacaa tatctatact tagaggttta aaagaaagat ttgagataca
     2401 tcatggagta agaatacatg ataatgctat tgtagcagca gcaaaacttt ctcatagata
     2461 tatacaagat agatttttac cagataaagc aattgattta attgatgaag ctggagctat
     2521 gattcgtagt gaaatagatt cattgccaac agaattagat gttgttagaa gaaagttatt
     2581 tacattagaa actgaaagag aagcattact taaagaaaat gatgataaaa gtaaatcaag
     2641 attggatgat attcaaaaag aaatagcaga gttaaaatct aaaaatgatg aaatgacagc
     2701 aaaatatgaa aaagaaaaga gtcaaatatt agatattaaa aacttaaagg ctcaattaga
     2761 cgaagctaaa ggtaaagctg aaaagtatga aagagaatat gatttcaaca aagcagcaga
     2821 ggttaaatat ggtgaaatac ctaagttaga agaacaaata aagcagtacg aagaaaatat
     2881 gaatgatggt tctgaaaatt ctcttttaaa agaagaagtt actgaagaag aaatatctag
     2941 cattgtatca aaatggacag gtataccagt aactaaacta gttgaaggag aaagagaaaa
     3001 attacttaaa ctagaagatg aacttcataa aagagttatt ggacaagatg aagcagttac
     3061 agcagtttca aatgcagtaa tacgtgcaag agcaggatta aaagatgaaa gaaaaccaat
     3121 tggttcattt attttcttag gccctacagg tgttggtaaa actgaacttg caaaaactct
     3181 tgctagaaat ttattcgata gtgaagataa tataataaga atagatatgt ctgaatacat
     3241 ggaaaaacat gctgtatcaa gactagttgg acctcctcca gggtacgtgg gatatgaaga
     3301 gggtggtcaa cttacagaag cagttagacg tgctccttat tctgtaatac tatttgatga
     3361 gatagaaaaa gcacatgagg atgtatttaa catgttctta cagatattag atgatggtag
     3421 acttactgat aataaaggta agacagtaga ctttaaaaat actcttataa taatgacttc
     3481 taatatagga agtaattatc tattagaagc aggaggaaat ataacagaga ctactaacaa
     3541 tttagtaatg aatgaaatga agcatagatt taaacctgag tttttaaaca gagtagatga
     3601 tattataatg tttaagcctt tagaccaaga aaatatcaag aaaatcatag atatatttat
     3661 gaaagattta aaaaatagat taaaagaaaa agatattact attgaagtta caaattcagc
     3721 aaaagatgtt atggtgagag aaggatatga ccctgtatat ggagcaagac ctcttaagag
     3781 atatatagga aatacattag aaactataat tgcaaagaaa cttattgctg gagatgtgta
     3841 taatggatgt actatagtta tagatggaaa agatgaaaat attgaagttt tagttaaata
     3901 ataaaagaat cagttgagca taatatacta aatattttgc tcagctgatt tttatttata
     3961 aactatagga aaagaaatat tttatcaaat taataaaaca tttttaagtc aatatgtcca
     4021 aattaaattt aactcaaagg aagtatt
//
LOCUS       DACXMZ010000271         4021 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.272, whole genome shotgun sequence.
ACCESSION   DACXMZ010000271 DACXMZ010000000
VERSION     DACXMZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.272"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            127..330
                     /locus_tag="JD016_17655"
     CDS             127..330
                     /locus_tag="JD016_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU5001443.1"
                     /translation="MISYAPLWKLLIDKKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            494..1648
                     /locus_tag="JD016_17660"
     CDS             494..1648
                     /locus_tag="JD016_17660"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAU5001444.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1661..2665
                     /gene="rseP"
                     /locus_tag="JD016_17665"
     CDS             1661..2665
                     /gene="rseP"
                     /locus_tag="JD016_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAU5001445.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2848..3903
                     /gene="ispG"
                     /locus_tag="JD016_17670"
                     /gene_synonym="gcpE"
     CDS             2848..3903
                     /gene="ispG"
                     /locus_tag="JD016_17670"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAU5001446.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKRVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
BASE COUNT         1563 a          399 c          780 g         1279 t
ORIGIN      
        1 tttaacatat ttatatttaa taaactataa ttgaaatcta tttagataaa taattattta
       61 tctaaataga tgaaaatttt ccaaaataga taaaatgtga tagaatatat tttgcgaggt
      121 gataacatga tttcatacgc tccattatgg aaattgttaa ttgacaaaaa aataaagaaa
      181 atggaatttg taaatatatc aggtataagt atttctgttt taggcagact aggaaatgat
      241 aaatctgttt ctatggatac aatggagaag atatgtttag cactagactg taaaatagaa
      301 gatgttgtgg aaataaaaaa gggcatttaa taatactaga atattttata atattccaca
      361 aaatacaaaa aataatagat taattttaat ctgtggaata atatatgaaa tttctatgaa
      421 tagtaaaaat aattgtttta gtagtgttat aatgttttga cagggtatat ttattattag
      481 aaaggattat tatatgaaaa agatatcaat tttaggttct acaggctcta taggtaaaca
      541 aactttggat gtggttagag aacatagaga taagtttgag atagttgcaa tatcagctaa
      601 cagtaatatt gaattattgc tagaacagat agtagagttt aaacctaaat atgtaacagt
      661 ttttgaggaa aataaagcat taaaattaaa agaaatgttg cctaaaaata tagaaataga
      721 agttttagca ggaatggaag gattaaaaat aatatcatca cttgatgaag tggatgtcct
      781 tttaactgct gtagtaggta tgataggatt agtcccaact ctttgtgcta taaagaaggg
      841 aatagacata gcattagcga ataaagaaac gttggtaact gctggtgaac ttgtaatgaa
      901 agaagcagaa aaatacaatg taaatattct tcctgttgat agtgaacata gtgctatatt
      961 tcaatgtcta aatggagaaa ataaaaaaaa tatagaaaag ataatactta cagcatctgg
     1021 tggtccattt agaggaaaga aaaagggaga acttgcaaat ataactaaaa atgaagcttt
     1081 gaagcatcca aattggagta tgggaagaaa aataagtatt gattcttcaa cgcttatgaa
     1141 taaaggactt gaagtgatag aagctagatg gttatttgga gtagaacaag aaaatataga
     1201 tgtagtagtt cacccacaaa gcataattca ttcaatggtg cagtatacag atagctcaat
     1261 aattgctcaa ttaggatgtc cagatatgag actaccaata cagtatgctc ttacatatcc
     1321 agatagaatg gaaagtagct ttgaaagaat gaatttttca aaatttagta ctttaacttt
     1381 tgaagaacca gatttagaaa cttttccatg tttaaaattg gcatatgagt gtttaaaaat
     1441 gggaggaact tattcttctg ttttaaattc agcaaatgaa gttttagtaa gtgaattttt
     1501 agaggataaa attggtttct atgacatacc atattacata gagaagactt tagaagttca
     1561 tagtagcata agtgaaccaa cattagaaga aatcttagaa acagatagat ggagtagagc
     1621 ttatgttgca aatctgataa aaaaatagga aggtgaattt atgactataa tagccgcatt
     1681 aatactattt agtataattg tattaataca tgagttagga cattttatat ttgcaaaaag
     1741 aagtggtatc aaagtaaatg agttttctat aggtatgggg cctaagatat atagtgtaaa
     1801 aaaagataca gagtattcta taagagcact tccaattggt ggatatgtca gcatggaagg
     1861 tgaagatgaa gaacaaataa gtccaaattc ttttggaaac aagtctatat tacaaagatt
     1921 tagtactatt gtagcaggac caatattcaa tataatatta gcagcaatac ttttagtccc
     1981 agtatttttg tatataggtt ctccaacgac taaattagga aagataatgc cagatactcc
     2041 tgcacaggct gtagggcttc aagtaggaga taagataaat aaaattaatg gtaattctgt
     2101 taagacttgg gatgaagttg caaatataat aaatacttct tctggtggag aattaaaact
     2161 tagtataact agagatggaa gtgataaagt tgtaaatgta acacctaaaa ataacaatgg
     2221 aaagtatgaa atagggattc aaccacaaag agaaaaagac tttttggggt caatagtaaa
     2281 tgcatgtaaa actactgtag atatgacaaa acagatgcta acatttctag gtcaaatgat
     2341 tacaggacgt gtaccaggag ggattggcaa tgctgtagca ggaccagtag gtgttattgg
     2401 tatggtatca gatgctgctc gtacaggcat cataaatgta gtgtatttag cagccgtcat
     2461 aagcttaaat ttaggtatag taaatctatt accaatacca gcacttgatg gatggagaat
     2521 acttatgctg ttactagaag ctgttagagg tggtaaaaaa ctcgacccaa ataaagaagg
     2581 catgataaat gttgttggtt ttggagcttt aatgttattt atgcttttta tcacatataa
     2641 agatatacta agattatttc aatgatttag ttttttagag attttaagaa aataggctat
     2701 gtagttttaa ataagaataa attaattttt atatatgaaa tttattctta tttaactaga
     2761 tagcttttta atttataata tacatgtagg ttataatatt ttaatattta aagagattca
     2821 atataaaata atatatagag gtggaatatg agttacgaga gaagaaagac gagagaagta
     2881 agtgtaggaa ctgtaaaaat aggaggagaa aatcctataa gtatacaatc tatgacaaat
     2941 actgatacaa gagatgcaga tgctacaata gctcaaataa agagattaga ggaagctggt
     3001 tgtgatatag ttagagttgc tgttcctgat attaaagctg ctaaaaatat agcaaagata
     3061 aagtctagtg taaatatacc aattattgca gatatacatt tcgattataa gctagccctt
     3121 gaagctatag aacaaggtgt ggatggaata agaataaatc ctggtaatat tggaagtata
     3181 gagagagtta agatggtagt tgagaaatgc aaggaaagaa atttaaaaat tagaattgga
     3241 gttaatggag gttctttaga gaaagaactt ttaaaaaagt atggctctgc aactgctgaa
     3301 gcattagttg aaagtgcaat gggacatatt aagattcttg aagacttaga ttttcataat
     3361 atagttatat ctctaaaatc atccgatatt tataaaactg ttgatgcata tgaattaata
     3421 tcaaaaagag ttgattatcc tcttcatatt ggaattacag aatctggaag tgttcacaaa
     3481 ggaacaataa agtcttctat aggggtagga gcacttcttc ttaaaggaat tggagatact
     3541 gttagaatat ctttaacagg cgaccctgtt gaagaggtta ttgttggtaa acagatttta
     3601 agaagtttgg gacttttaaa tgataagatt aaagttatat catgccctac atgtggtaga
     3661 tgtaatatag acttaattag tgttgtaaat gaggttgaag aaaagattgg tagtatggaa
     3721 aaaaatatca ctgttgcaat tatgggctgt gctgtaaatg gacctggtga agccagagag
     3781 gcagatatag gtattgctgg gggtaaaggt gaaggtcttt tatttaaaaa gggtgaaata
     3841 gttagaatga ttgatggtaa taaattagtt gatgagttgc tagaagagat tgaaaaatta
     3901 tagtatacaa ttcatatata tattaattga ctagattttt aaatactttt gtaataagtt
     3961 tttatttaca gtatttaatt atctagtcat tttttatttt ataaacttca ttaaatgtaa
     4021 g
//
LOCUS       DACXMZ010000272         4004 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.273, whole genome shotgun sequence.
ACCESSION   DACXMZ010000272 DACXMZ010000000
VERSION     DACXMZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.273"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            121..816
                     /locus_tag="JD016_17675"
     CDS             121..816
                     /locus_tag="JD016_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAU5001447.1"
                     /translation="MAYDFYLDGVQLPITPGKLEIKTTNKNKTVDLINDGEVNILKTP
                     GLSEISFEAEFTHNKLPFYKGAFKDVQFFLSKLELLKTDCKPFQFIVSRQLGNKVLFN
                     TNRKVSLEEYNIVEDADNGSDFKVAIKLKQYRDYSTKKLVPAPPKKTNETGRPSVKIE
                     PKRVDSVNAPSNKTYTVKSGDSLWSICQKQLGNGSLYKKVYELNKTMLDKANKGKNLS
                     KYTIYKGQVLKLG"
     gene            809..2701
                     /locus_tag="JD016_17680"
     CDS             809..2701
                     /locus_tag="JD016_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HAU5001448.1"
                     /translation="MDDELVLANDRDVRLVIAHWEDFYEPVVLDNITWEIERRGSPSK
                     LEFTIVMDDILQFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRIEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRLTLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEVEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLIPVFLDLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNSNNKDEKKNGTAQNTTKTNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLKKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSSTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            2715..2975
                     /locus_tag="JD016_17685"
     CDS             2715..2975
                     /locus_tag="JD016_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HAU5001449.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPIKIVFGTIESISPLKVKIEQKL
                     SIGEFFLIQTDTFKRYTDKKVGDKLVLIRMQGGQQYLILDRM"
     gene            2980..3399
                     /locus_tag="JD016_17690"
     CDS             2980..3399
                     /locus_tag="JD016_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HAU5001450.1"
                     /translation="MIPTDNIDYDIEDVSIINFDVRQEPSKTFKLHIEKNRVDGICND
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNIKGKVHCKFMVHTKYGNIKAEKVVSV"
     gene            3400..>4004
                     /locus_tag="JD016_17695"
     CDS             3400..>4004
                     /locus_tag="JD016_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HAU5001451.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAVELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISEGIYKMKCETAGPIFELGQLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKIQDIGGVKVYPVW"
BASE COUNT         1630 a          429 c          773 g         1172 t
ORIGIN      
        1 aatataaaca acataagtaa agatacagat gtcgacggta tagttaatgt actaacagaa
       61 aaattaaacg atgctatggt tgtatcagca gaaggaatag tttagagagg aggtgtaatc
      121 gtggcttatg atttttactt ggatggagta caattaccaa tcacacctgg caaattagaa
      181 ataaaaacaa caaataaaaa taaaactgta gaccttataa atgatggaga agtaaacata
      241 ttaaagactc ctggtctatc tgaaataagt tttgaagcag agtttacaca caataaatta
      301 ccgttttaca aaggtgcttt taaagatgtt caattctttt tgagtaaatt agaactatta
      361 aaaacagatt gtaagccatt ccaatttatt gtatctcgac aacttgggaa taaagtacta
      421 tttaatacaa atagaaaagt atctttagag gaatataaca tagtagaaga tgcagacaat
      481 ggttcagatt ttaaggttgc aataaagtta aaacaatata gagattactc aactaaaaag
      541 ttagttcctg ctccacctaa aaaaactaat gagactggta gaccaagcgt aaagatagag
      601 ccaaaacgag ttgattcagt caatgcacca agtaataaga cttatacagt caaaagtggt
      661 gattctcttt ggtcaatttg tcagaaacaa cttggtaatg gttcactgta taagaaggta
      721 tatgagttaa ataaaacaat gctagataag gcaaataagg ggaagaattt aagtaaatac
      781 accatttata aagggcaggt gttaaaactt ggatgatgaa ttagtgttag caaatgatag
      841 agatgtaagg ttagttattg ctcattggga agatttctat gagccagttg tccttgataa
      901 cataacatgg gaaatagaaa gaagaggttc accttctaag ttagagttta caatagtcat
      961 ggatgatata ctacaattct gtgaaggtaa ctctgtaagg ctgtattata aaggaatagg
     1021 catattttat ggatatatat ttcagaagaa aagagataaa gaaaatcaca ttaaaattgt
     1081 tgcttacgac cagttgagat attttaagaa taaagatact tatgtatata gtaataaaac
     1141 tgcatccgaa cttgtaaaaa tgttagctaa agattttaat ttgaaataca atgttataga
     1201 agatactaag tataaaatat ctaggataga agaaaataaa acactctttg atatgatatt
     1261 aactgcacta gatgatactt taagagagaa aaaagaaatg tatgttttgt atgatgattt
     1321 tggaagatta acactaaaaa atgttgcttc tatgaaatta gatacagtta tgaataatga
     1381 tgtaatagaa gattttgact ataattcaag tatagatagt gatacttata caaaaattaa
     1441 acttgtgaga gacaatgaag aaacaggaaa aagggatgtt tatattgcac aagattctac
     1501 gcatatgaga agctggggaa tacttcaaat gtttgataca gttgataaaa atatgagtga
     1561 agtagagata aaacaaaaat gtgatatact tctaaaatta tataataaga aaactaagtc
     1621 attaagttta aaaaatgtgc ttggtgacat tagagttaga gcaggttgtt taatacctgt
     1681 ttttttagat ttaggagata ttgagttaca aaattatatg ttagttgaga aagtaaaaca
     1741 tacatttgaa aataattcac attttatgga tttaactctt gttgatggag acgaatttgc
     1801 ttcttattct tcatcttcat atagtagtgg aaatagtaat aataaagatg aaaagaaaaa
     1861 tggtacagca caaaatacta cgaaaacaaa tacaggtaaa aaagttcctg ctatatttac
     1921 tgcatattat ccagggaaca atgcaatgga aggtggaaaa acagattgca atggaaagcc
     1981 acttgatgta aaatcaagaa ctgttgctgg tccaatgaat cgagaaggag ttaagaaaac
     2041 ttggtatact gatgattttt taaaaaaaca tccagttttt gaatatggag ataaagtaaa
     2101 aattatactt cctggtactg cctatgacaa caaagtatat acagttaaag ataatggagg
     2161 aagaatatat gttgaaacaa acggaacata tcatatagac atactattag ctaatgctag
     2221 tgaatgtaaa aaatttggta gaaagaatgg atatataatt ataggtggag atgaagaaca
     2281 aacatatcaa gttgaaggta ataaccaaag tagtacaaat aataactcta aagaagataa
     2341 attaattagt atagcaaaaa gtaaactggg ttgtaattat gtgtatggag cagaaggtcc
     2401 taataatttt gattgcagtg ggtttactca atggtgttat aaacaaatag gtataaaaat
     2461 tcctcgtact gcttctgcac aaagtaaagc aggaaaagca gtagatttaa aagatagaag
     2521 caagtggaaa gcaggagatt tattgtgcag aattggtgga ggaagtagta atcatgttgt
     2581 aatgtatatt ggaaacaatc aaataattca ttcaccacaa acaggagatg tggtaaaaat
     2641 agagtctgtt aattcatata gaaaaggaaa agcatataca catgtgagaa gatttatata
     2701 agtgaggtga taatatgagt caagatttat tacagataat aaaaaaagca gcaatggatg
     2761 cagtagaaac aagtaatcca ataaaaattg tatttgggac aatagaaagt attagtcctc
     2821 taaaagttaa gatagaacaa aaactatcta ttggtgaatt ttttctaata caaacagata
     2881 catttaaaag atatacagat aaaaaggtgg gagacaaatt ggttttaatt cgtatgcaag
     2941 gagggcaaca atatttaata ttagatagga tgtgatgaag tgataccaac ggataacatt
     3001 gactatgaca ttgaagatgt atcaataatt aattttgatg taaggcaaga accaagtaag
     3061 acatttaaac ttcatataga aaaaaacaga gtagatggta tttgtaatga tgtggaagca
     3121 ttaaaacaga ccatcttttt aattttaaat actgaaaggt atgagcactt aatatacagt
     3181 agaaattatg gagtcgagtt gaacgacctt attggagaac ctatatcctt tgtaatacca
     3241 gagttagaga gaagaatcaa agaagcacta attcaagatg atagggttga aaatgtagat
     3301 aattttgagt ttcaaaatat aaaaggaaag gtccattgta aatttatggt ccataccaaa
     3361 tatggaaata taaaagcaga gaaggtggtg agtgtataat tgtttgagtt aatgacattt
     3421 gaaaatataa ttaaaagaat gttagatagt gtaccagata cttttgataa aagggaaggt
     3481 tctataatat ataatgctct tgctcctgtt gcagtggagc ttacagaaac atacattgca
     3541 atggatgaat tactagacca aacattcgta gatactgcta gttattacta tttagagaag
     3601 agatgtaaag agagaggaat tacacctttg ccagccacta atacgattgc taaaggcgtt
     3661 ttcaatatag atattccact tgattctagg tttaatttag gagagtataa ctatgtagca
     3721 attgagagaa tatctgaagg tatatataag atgaaatgtg aaactgctgg acctattttt
     3781 gaacttggtc agttgattcc aattgaatat atagacggtc ttgaaacagc agagttaaca
     3841 gaaatactga taaatggaga agatgaagaa tctgaggaca gtttaagaca aaggtactat
     3901 gatagtttaa attcacaaag tttcggtgga aatatacaaa actataagga tgaagttaac
     3961 aaaatacaag atattggagg agttaaagtt tatccagttt ggaa
//
LOCUS       DACXMZ010000273         3982 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.274, whole genome shotgun sequence.
ACCESSION   DACXMZ010000273 DACXMZ010000000
VERSION     DACXMZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.274"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..282
                     /locus_tag="JD016_17700"
     CDS             <1..282
                     /locus_tag="JD016_17700"
                     /inference="COORDINATES: protein motif:HMM:PF02142.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001452.1"
                     /translation="NSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKIRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            540..1589
                     /gene="carA"
                     /locus_tag="JD016_17705"
     CDS             540..1589
                     /gene="carA"
                     /locus_tag="JD016_17705"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAU5001453.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            1782..>3982
                     /gene="carB"
                     /locus_tag="JD016_17710"
     CDS             1782..>3982
                     /gene="carB"
                     /locus_tag="JD016_17710"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HAU5001454.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTVEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYL"
BASE COUNT         1647 a          403 c          789 g         1143 t
ORIGIN      
        1 aatagcttaa gagaaaatgg aatagaggct gatatagtaa atagagtaga agaatctaga
       61 ccaaacatat tagatgctat aagaaataaa caagttgata tagtaataaa tacaccaact
      121 aaaggaaatg actctacaag agatggattt aagataagaa gaacagctat tgaattttct
      181 actgaaataa tgacatcttt ggatacacta aaagctttag tagaagttaa gaagaaacat
      241 ttaaataaag atgagttaaa agtttacaat atagctgaat agaaattaag ttgattgtat
      301 agtttaagaa tttaattatt aaataaaaaa aaagaggtta ttttttctag gagtttttac
      361 ttttgagata acttcttttt tttaagaaat ttgtagtata atagaaaaag ataaaaaata
      421 aatgtaaata agtataaaac tagaatgtaa aagtgaaaaa tagatataga tatttaaaag
      481 cttatattta gttaatgttc tttagtttgt ctaaagagaa ttataggagg tactgtagta
      541 tgaaagcaag gttaatatta gaagatggaa cagtttttat aggaaaggca tttggatatt
      601 tagaagaaag tgtaggagaa gtagtattta atacttcgat gataggatat ggtgaagttt
      661 taacagaccc ttcttactat ggtcaaatag ttactatgac ttatcctctt gtaggaaatt
      721 atggtataaa tttatcatca gcagagtctg aaaaagttca agtaaaggga ttcatagtaa
      781 gggagaaaag tgattctcca agtaatttta gatgtgaaat tgatatagac caatatttaa
      841 agcaaaataa agttatcgga cttgaaggaa tagatacaag agctttgact aagatactta
      901 gaaataatgg aacaatgaaa ggtataatta cattagaaga cagtaaatta gaagatgtta
      961 aacataaatt ggataagttt tcaaatacag aagcagttag aactgttaca agaaaagaag
     1021 tagagcatat aaaaggaaat ggtccaaaag tggctgttat ggattttgga gtaaaaagaa
     1081 atatattaag atcatttata gctcgtggat gtgatataac aatatttcca gctacaacat
     1141 ctccagaaga tgtattgagt ataaaccctg acttaatatt cttgtcaaat ggacctggag
     1201 acccagaaga cttagaagat gttatagaaa atataaaagc tcttattggt aaaaaaccta
     1261 tagttggaat atgcttagga catcaacttt tagctttagc tcttggagga aaaactgcta
     1321 aacttaaatt tgggcataga ggtggaaatc atccagttaa agatttggaa gaaggaaaag
     1381 tatttataac ttctcaaaat catggttatt atgtttcaga agttcctgaa caaatgaaag
     1441 tgacacatat aaatttaaat gataatactg ttgaaggaat gcgacatgag aagctagatg
     1501 tatacagtgt tcaataccat cctgaagctt gcccaggacc aaaagataat gactatattt
     1561 ttgataaatt tttagaatta gtaaaataaa taacattaga atagctaact aaaaaacaag
     1621 aataacttaa atcaaaaagt ttggtttaat taacatagat gtattaaata gatgtattga
     1681 tgagtaaatt tgaacttaag ataaaataaa gtaattaaag ttgattgtat agatgatgaa
     1741 gataagaact gtgaaaattg tttagataag gagaaagagt tatgcctaaa ttagatagta
     1801 taaagaaaac tttagtatta gggtcagggc caataataat aggtcaagct gcagagtttg
     1861 actattcagg aacacaggcc tgccaagcat taaaagaaga gggaattgaa gttgtattag
     1921 taaatagtaa cccagctact ataatgactg ataaagaaat tgcagataaa atatatatag
     1981 aaccattaac agtagaattt atagaaaaaa taatagaaaa agaaagacca gacagtttac
     2041 ttgcaggtat gggtggtcag acaggattaa acttagctgt agaattacat gatgctggta
     2101 tattagataa atataatgta aaagttatag gaacatctat agaatctata aaaaagggcg
     2161 aagatagaga tttatttaga gaagtaatga aagaaataaa tcaaccagta atagttagtg
     2221 atatagttac aaatttagaa gcaggtcttg agtttgccaa taagatagga tatccagtag
     2281 ttgtaagacc agcatatacg ctaggtggaa ctggtggagg tatagctgat actgaggaag
     2341 aattaagaga aatactttca catggactac agctaagtcc tgttggacaa gttcttcttg
     2401 aaaagagtat aaaaggttgg aaagaaatag aatatgaagt aatgagagat ggaaatggaa
     2461 actgtataac tgtatgtaat atggaaaatg tagacccagt tggagttcat acgggagaca
     2521 gtatagttgt tgctccaagt caaactctaa gtgatgaaga gtatcaacta cttagaaagg
     2581 catcaataga cataataaat gcaatagagg ttcaaggtgg atgtaacgtt caaatagctt
     2641 taaacccaca tagtttagaa tatgcgataa tagaaataaa cccaagagtt agtaggtcat
     2701 cagccctagc atcaaaagct acaggatacc caatagcaaa agtagcagct aagatagctt
     2761 taggttatac tttggatgag atagaaaatg cagtaactaa aaaaacttat gcttgttttg
     2821 agccaacatt agattatgtg gtagtaaaaa taccaaagtg gccatttgat aaatttagac
     2881 atgccaatag aaaattagga actaagatga tggcaactgg tgaaatcatg agtataggaa
     2941 gtaactttga ggctgctatt cttaaaggta taagatcact tgaaacagga aaatattcat
     3001 tggttcatgc tccatcagaa ggtagaacat tagaagaatt aaaggctaga gtagtagtac
     3061 ctgatgatga gagactattt gacttagctg aaatgataag aagaggctat aaagtagaaa
     3121 tgatatcaga aataactggt gttgataagt ggtttataaa taaatttaaa tggatagtag
     3181 aacaagaaga aaagttaaaa gttctaaaaa tagaagactt agataaagaa tatttacatg
     3241 aattaaagaa aaagggattt tctgataaag gtatatctga cttaatgaag ataagcccag
     3301 aaaaattata tgaattgaga agtttatata atatacaacc agtttataaa atggttgata
     3361 catgtggagg agaatttgaa gctctatcac cttattacta ttcaacttat gaacaatatg
     3421 atgaggtagt agttagtgat aagagaaaag ttgtagtatt gggttctggt ccaataagaa
     3481 taggtcaagg aatagaattt gactattgtt cagttcactg tgtaaaatct ttaagaaaaa
     3541 tgggaataga aactataatt gtaaacaata accctgaaac tgtaagtacg gattttgata
     3601 catctgataa gttgtatttt gaaccattaa cagaagaaga agttttaaat ataatagaaa
     3661 aagaaaaacc agaaggtgta atactacagt ttggtggaca aacagctata aaattggcta
     3721 agtttttaca tgaaaaaaat atacctatac tgggaacaga ctttagagat atagatgcag
     3781 cagaagatag agaaaaattt gatgatttac tagagagact agatataaat agaccaaaag
     3841 gaaaaggtgt atggacaact aatgaaggtg ttgaaattgc taaggagtta ggttatccag
     3901 tattagttag accatcttat gtacttggtg gtcaaggtat ggaaataact tataacgaag
     3961 aaaaactaat tcagtatttg ga
//
LOCUS       DACXMZ010000274         3973 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.275, whole genome shotgun sequence.
ACCESSION   DACXMZ010000274 DACXMZ010000000
VERSION     DACXMZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.275"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            31..660
                     /locus_tag="JD016_17715"
     CDS             31..660
                     /locus_tag="JD016_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HAU5001455.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            644..1042
                     /locus_tag="JD016_17720"
     CDS             644..1042
                     /locus_tag="JD016_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YtfJ"
                     /protein_id="HAU5001456.1"
                     /translation="METTLETIKGSIDANTIIGDPIKTDTTVVVPISKVTIGFGIGGG
                     EYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETRIMSLDSNINL
                     VDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1290..1730
                     /locus_tag="JD016_17725"
     CDS             1290..1730
                     /locus_tag="JD016_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAU5001457.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1746..3197
                     /locus_tag="JD016_17730"
     CDS             1746..3197
                     /locus_tag="JD016_17730"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAU5001458.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALILLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3288..3956)
                     /locus_tag="JD016_17735"
     CDS             complement(3288..3956)
                     /locus_tag="JD016_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HAU5001459.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1659 a          358 c          678 g         1278 t
ORIGIN      
        1 aaatagtaat tttttgatga taatataaac atggagctta atgaattatt taattttaag
       61 tatttaataa tagctgtaat ttttattata agtgtaacta tgttgatttt aatatctcat
      121 ataaacatct taataatagc tgatataaaa aacaaagata tttgtttaaa gcttaatata
      181 aaatacatgt ttaatttaat aaatataaat agacaacttt atccagctga aaatagtaaa
      241 aataatgata aaaaagaggg gatgaagaat aatatagatt caagtatatt attagctgat
      301 gaccttttga gcatttatag attattaaaa aagataaaaa tacatgaact ttattcaaat
      361 attaattttg gaactggaaa tattggctta acatcttctg tatatgtatt gataaatact
      421 ttgtatggaa atttatttaa tatgattgat gcagaaaaaa tgtatttaaa tgtaaatcca
      481 gattttacaa aagattatgt tttaggaaat ataaggatac atataaggcc aagaataaaa
      541 gctttattta atattataat aatgattaat aaaattatga ataaaaataa aggaaataag
      601 gaaggtgata gtaatgagag caacaggttc gatacagagt cttatggaaa caactcttga
      661 aacaattaaa ggttctatag atgcaaatac tattatagga gaccctataa aaacagatac
      721 tacagtggtt gtacctatat caaaggttac aattggcttt ggtataggag gtggagaata
      781 ctcaaaagga tatgatgata aagataggga aattgaatta aaaaatgaaa aatcagatac
      841 gaattttgct ggaggtagcg caggggctat atctgtacaa ccagtagcat ttgtagtagt
      901 tgaaagtgga gagactagaa taatgagttt agatagtaat atcaatctag tggataatat
      961 attatctata actcctagag tattggagaa aattcaaaat atatcacaaa gtaataagaa
     1021 tcaagataaa aacaatatgt aagaataaaa tatttttaaa tatgtatata gttttaagta
     1081 aagtagtggg gttaatataa atcccactat tttgttgtgt actttaaaca tttatatcta
     1141 ataagatttt agtttaaaac aatttaaata gataataaag ttgatatata gtataattat
     1201 aaaaagaaaa gaagtattat attatagata tataaacgca tttgagatat aagagggagt
     1261 tataaatcat tagttttgag gtgaatgtta tgagcgataa agaattatct attttgttgt
     1321 tagaaagtat gaataatttt cataatttaa taaagattat taataacgaa agatataaaa
     1381 aagataaggt tcttacagaa agacagttct ttgccttagt aaaaataaga aaacatgata
     1441 aaatagagct taaaaatcta agtagagatt tgcatgtatc tacttctagt ctgtgtatac
     1501 tacttaataa attggttgag caagagtatg tatataggga agaagatagt agagatagac
     1561 gaaatacttt ttatggaata actaaaaatg gagaaaagat attggataat gaaatactaa
     1621 agtttgtatc tataataagt gataaaatgg attgcctaga tatagataat aaagataaat
     1681 tatttacttc tttggaagag tctaaaaata taatagaaca attattttaa aaaggagaat
     1741 ttattatgaa tataaagtat tctaaagaga tattaaaaag tttagaaaat gctataacag
     1801 tagtagctct atttgaggga gaagatgttt ctacgacatt tgataaaata aatatgataa
     1861 gcagaaattt taattctaaa gaaaatagta tagatactgt aacattaatt gaaaataaca
     1921 atttggtaaa tgtttgtctt gttggttttg gtaagagaga gttattgaat agagaaaaaa
     1981 ttagaatcat aggtgggaat ttgtgtaaaa aattaaaaaa tatattgaaa aatgaagcat
     2041 ataagagtag tgatgttaat gtactgaatc ttaatttaaa tagtgatgaa ataggttgtt
     2101 ttgtagaagg gatattactt ggaaattata agtttgataa gtataagaca aaaagtaagg
     2161 attttgaaag aaaagaagtt gatactataa ctatatttac tgaaaaagaa gttgagtgtc
     2221 aaatagaaaa ggctaaaata ttagctaatt caactataat agctagaaat ctagttaatg
     2281 aaccatcaaa cgtaatatat ccaaaaacat tagcagtaga aacagttaaa cttggtgcag
     2341 agtttgggtt taatgttgat gtttatgaag aagaaaaaat aaaggcattg ggaatggatg
     2401 cttttttcgc tgtttcaaga ggttcaatta ataaaccaag attcatagta atgagatact
     2461 ttggagataa agaaagtaca gatatattag gtctagtagg taaaggattg acttatgata
     2521 ctggtggata ttctttaaaa tcaaatgctt ctatgctaga tatgaagaca gatatggctg
     2581 gtgctgcgag tgttataggt gctatgtgtg ctatatctca aagtaaatta aagaaaaatg
     2641 ttatagcagt agtggcagca tgtgagaatg ctttatcagg aggttcatac aaacctggag
     2701 atataattag ttctatggca aagaaaacta ttgaagtttt aaatacggat gcagaaggta
     2761 gacttacatt agctgatgca atatactaca ttataaacaa tgaaaaagta acaaaggtgg
     2821 ttgatgtagc tacattaact ggtgctgctt taatattgtt aggtaatgtc gcaactccta
     2881 tagtaacaaa taatgatgat ttttattgtg aattagaaaa agctgccact ttatcagggg
     2941 aaagggtatg gaagatgcca atttatgatg aatttaaaga tatgataaaa ggagaagaag
     3001 cagaccttaa aaacactggt ggtaaaaatg ctggatgtat aacagcagga gcttttattg
     3061 gtgaatttgt tgggaatact ccttggatac atatggatat agcggggaca agtacatcat
     3121 caaaatcaat tggatacaaa gcaaaggggg ctacaggaga acctgttaga acattgtact
     3181 atttggcaga aatctaaaaa acaattataa aatataattt ataaagccac gtggatattt
     3241 ttgtaattta agattatcta tgtggcttta ttttttattt aaataaatta tttactcttt
     3301 ttcttttttt caagtataaa agtatctcta ttaaataaaa gagcaggtat gtacataata
     3361 ctagtaacaa aaataaacgt aaaaatagca gttgtgctat gacttaaaaa accataatag
     3421 tacctagagt tggtaaatgt gtttagtaaa aatatgaatg aaaaataaat aaaacatata
     3481 gctaagttat ctctaagtgc atatctgtta actctaaatt ttctaacatc aaccatatat
     3541 atatctgcat aaattatcaa gcacttagag aagataaaga taaaatatat taagctagga
     3601 aattcatagc ccaagttttt agaaaaaatg agttctccaa ctgaacaaac taaactccaa
     3661 gaaaaaaata tattaaaaag atgatactgc ttaaaaatta gtatggctat acacagatat
     3721 atacaaatat aattgattcc aattggtaaa gagtttagag tactataatt acctgaagaa
     3781 ataagtaata attgctctag agccataatt attacaagta atgtacaaat tatcttctca
     3841 actgtatagc tataaggtaa aatatttttg gttagttttg gacaatagta taaaaataat
     3901 ccaaatataa agagacttac aaattgttca ctagaaaaaa gaaaaatttt aaacatataa
     3961 cattctctcc tat
//
LOCUS       DACXMZ010000275         3966 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.276, whole genome shotgun sequence.
ACCESSION   DACXMZ010000275 DACXMZ010000000
VERSION     DACXMZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.276"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(331..741)
                     /locus_tag="JD016_17740"
     CDS             complement(331..741)
                     /locus_tag="JD016_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001460.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(957..2339)
                     /locus_tag="JD016_17745"
     CDS             complement(957..2339)
                     /locus_tag="JD016_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HAU5001461.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVEVGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2350..2709)
                     /locus_tag="JD016_17750"
     CDS             complement(2350..2709)
                     /locus_tag="JD016_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAU5001462.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEIAKLKQIVEKLK"
     gene            complement(2914..3693)
                     /locus_tag="JD016_17755"
     CDS             complement(2914..3693)
                     /locus_tag="JD016_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAU5001463.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         1397 a          661 c          382 g         1526 t
ORIGIN      
        1 tgttaacatt ataaattggg tttaatactt ttactttgac tcgatttact tttattctac
       61 ttcttacttt atttttaact tagactttat ttttttatta cttatttgag tcttatttat
      121 tttattatca ctccatatgt tcaattacat ttgtctaaat tctttgatac ttttattaca
      181 taattttaac aagcttaagt gttatttggg tagtgcagtt gataattatt taaataatca
      241 tcaactgcat gtttatatca ttacagtagc ttaatgtatt taatcatact tacttaggat
      301 tttgaaatat cttagagatt atatttaaca ttagtcaatt gctgcataat tgtaagcaat
      361 tgaaatttga gacccatctt cattttctat agtgataggc ttcataattt ttaatccttc
      421 agtttttatc ttctctaatt cttgttccat tttcttaatt gtttcctcaa ggtctttttg
      481 acttacatct ttgactgcat tagggtcttt agcatagtct tttaattctt gaatatgttt
      541 agcatactct tcataagtat aatgctcaac accatacttt tccaattcag tagggtcagt
      601 tattcctgat tttattagag attcaagttt aggtaaagca atattagatg aatcatttcc
      661 atttgcaaat actgctccag ttcctcctag taccattgca gataccatca ttgcacttat
      721 aagggatttt tttattttca taaccaatct tcctttctta gagatttatt tgggtagtgt
      781 agtttgaaat gactattgca atagttatca accacatcct tagactatca cagtagtcat
      841 gtttatgtca agccttttta aaaaactttt caataaaaaa tactatctca aaacaaaatt
      901 aattattttg agatagtatt ttaattctaa atcttttata ttactaagat ttatggttac
      961 tctatccatg ttctatattg ttctttatta actgctaaca aattcccttt tcttgatatg
     1021 acaaagcgat tatcattata gccacttcgc tcaattgaaa cttctcctga atcagtataa
     1081 agtacatcct tactattact agatttattt acatacgttt cttcactatt ctcagaatta
     1141 gttactttgc tcatagatga ttcttcatgc tctctaaatt caattacaga tacttttgcc
     1201 gtactaaaat gagtctctcc atcactttta gctttactaa caagaatatc agattctact
     1261 ttgggagaat ataaatcagt tgatttacta cttattgaaa atccaatttt atcactttgt
     1321 tcattttttg ttaaaccaaa agattcaacc tcattaatct ctttattttc tgttgcaaaa
     1381 acaagacctg tcccacaaac taatacagtt gaaactaaaa aagtaacata agttgatttt
     1441 ttaaatttca taatattaac tattctcttt tctatttttg tctttccaaa tccactacat
     1501 attgtcattg aaaatggatt attctcctct gctagactta ttagtgtggt tgcataatca
     1561 gccctatatt cttcaccaag tatactcata accttttcat cacaactaaa ttctatgtct
     1621 ttatttagta aaaaaaacat cacccaaact aagggattaa accaatggac aacaagagct
     1681 acaagcataa ataactttaa cagattatca aagctattaa tatgaaccat ttcatgagtt
     1741 aaaacaaagt ctaaagtttg attattagca taattcattt gctttggcaa aataattcgt
     1801 ggttttaaaa taccagatgt aagtggtgtc ctagtaaatt cagatatgca aacaataaat
     1861 cttctaaaac ggctattctt acacatccat tttttaatga actcatgatt ttttatatga
     1921 attgaagttt taaaaagatt aattatttta aaatatctaa agcataaaag tatgctaaac
     1981 ataatagtaa ctataaaaga caaaatgccc aacatttcca acatcataat attttcaata
     2041 ttagacatta tttcaaattc gtcagaattg tttttacctg tagagtcact agctttctta
     2101 agagtataat tttctgttga tgtattaccc acttccacat caaaagtcga ttcaattata
     2161 ctataaatac taaaaccaga atttaaactt actggtatta gaagtttaaa taaaacaatc
     2221 aaccacaata atacaaatac ctttttaggt aattttttaa ataaacatgc tcttataaaa
     2281 ataactatta aaagtaatac tccagaagta aaactcattt ctaacaattt aatgaacata
     2341 aatcttacct cactttaatt tttcaacaat ttgtttaagt tttgcaatct catcacttga
     2401 aagattttct ttattaataa aagctgaaag aaatttttct gaagaccctc caaacatttt
     2461 attaattaaa gtttctgttt cctgtttttg tacttcctct ttgcttatta aagctttaca
     2521 aacaaaattc ggctcaattc tctctattgc accttttgac actaattttt ttataacggt
     2581 atacgtagtg tttctattcc atcctgtttc ttcttttaaa atttttacta actctccagc
     2641 cttgctttct cctctctccc atagaacttc cattacttta agttctgaat caaataattt
     2701 tatctccata taaaatcgct cctcaaatat agttatcaca atagtcatta ctataataaa
     2761 aaactatcat agtagtctat actatgtcaa tcatttattt tttaataata aaaactgtct
     2821 ttttaaaaaa ttcttataaa agacagtttt atcaatgtta tactttaatg tttaattaaa
     2881 gccatatttt atttaaatat acaactatct atcttagtac atagtataag atttttcttt
     2941 atctaatagt atatcctcta catctgggtt ttcttttact aattgtctat aaaaattaca
     3001 attagtaaca cccacatatg gaattagcca tagtagacct attccaagag tagctattga
     3061 aagtatatac catcctataa aactaaagtt tagtacaaat aatttaagta catttccttt
     3121 catcatatct aaactagcac ccattgattt aaatacaccc atattatcct tgtcttcaca
     3181 aattaagaaa gtagcttgag aaacatataa tagcaatata taaaccggta acataagaac
     3241 aagtattata agaactagag ttattccagt agaaataata gtccctattc caacattatc
     3301 acttgataaa tattcaattg ctccactagt aagtaaaact ccaaatgata aaaacgctcc
     3361 tataattgtg attataaaag aataaatata aacaataatt gattcaaata aataaaaacc
     3421 aaaacttctt aaataaattc tcccacttac aaagatatct cctatcttga ctttttcatc
     3481 tttagtaatt ttcaagaaca tacatgtaaa tcctgctgaa acgcaaaaag ctattacagc
     3541 cactacaata gtagtcaatg gttcaaaagg taatcctaca attgatatta atactatcaa
     3601 tgcaatatat actgccatag aaattgcaca tatcccccag tttccagata actgagcttt
     3661 agatactcgt tttaattctt gccttgatac cataaaaaac acccctttca ttttataaaa
     3721 aaacttttaa aaatataaat aatcttttaa cgtataatat taatatatca tattttcgac
     3781 tatataagat acaattattc accatgtttt atcaagatat gccataattg tattactagt
     3841 tttatatagt caacattacc aaatacatca aataaatttg attaattatt caacaagtaa
     3901 taaaataatt tctattaatt atactttatt tagtaaacaa attatttaat aaaaaaatta
     3961 cctaat
//
LOCUS       DACXMZ010000276         3934 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.277, whole genome shotgun sequence.
ACCESSION   DACXMZ010000276 DACXMZ010000000
VERSION     DACXMZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.277"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(198..581)
                     /locus_tag="JD016_17760"
                     /pseudo
     CDS             complement(198..581)
                     /locus_tag="JD016_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      807..904
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            959..1135
                     /locus_tag="JD016_17765"
     CDS             959..1135
                     /locus_tag="JD016_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001464.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            1361..1561
                     /locus_tag="JD016_17770"
     CDS             1361..1561
                     /locus_tag="JD016_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAU5001465.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            2180..2362
                     /locus_tag="JD016_17775"
     CDS             2180..2362
                     /locus_tag="JD016_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAU5001466.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIIF"
     gene            2433..3278
                     /locus_tag="JD016_17780"
     CDS             2433..3278
                     /locus_tag="JD016_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAU5001467.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(3502..3666)
                     /locus_tag="JD016_17785"
     CDS             complement(3502..3666)
                     /locus_tag="JD016_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001468.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         1488 a          483 c          451 g         1512 t
ORIGIN      
        1 atagtgttac ttatatttta tatatatggc tatttttcaa cattttacaa tatataacat
       61 ttattttttt aacttttgtc agtaaaatgt cagcaaaaca ctttatccac aatttttata
      121 taagttttcc acaacttatc accaatatta tccacataaa aaaggtagca cttatattat
      181 tagctacctt ttatttatta atctttatat ttccagcttt ttatccaact tcctatttct
      241 attatttttt cttcagttat ttcttctcca tcatgtaatt ttgaaattaa ttctgataaa
      301 ggattgttat gtaaacctct aaaaactctt tttccctcta ttttgagata ttctctctct
      361 tttattaata ctgtatagtg tacatgcctc ccaattctct atgatattaa aaaacctttt
      421 ttggttaatc tagctaaaag tataattgta gttgtatgct tccaaccata tgttttttct
      481 atttcatctg caacatcttt tgatattaca gttttataac cagtattcca tatgcatttc
      541 ataattttta attctgtatc tggtaatttt ttatttagca taatatattc cttctttcaa
      601 tttatgtcac ttcttcaaat ttatctagct taaattataa taggagcttt ttagaacaaa
      661 aactatattt aatttttacc aggtactcta cgtgtttagt ctgtttttaa gtgtaaaata
      721 atgttcatat ctataaattt atttaacttt caaacaatcc tgttgaataa taactaattc
      781 tattgtatta ttttagttgt agaataaaac taaattggca aaactagaga aatttagtga
      841 cgcaaagcta tagggactaa gacttatata aatatcttat gagttatgtc agccagttgc
      901 caaagagata gtgttctttt tgtttttatg aaagtttttt agggggataa taatttatat
      961 gtttggaaat aaaatggata aatgtacaca tgtattgact gcttatatta gcagttcgta
     1021 tgattattgt aattttttag atacacagtt agatgatttt atattagagt acggagaaaa
     1081 tgtagtagag tcttgtttac accaagtaat ggtattggta agtaagtata attagataga
     1141 cattgatttc tagaaaaccc tttttagttt tatctacatt tatattaata tatagcaatg
     1201 taaaacgttt aaatatattt ttaatattaa gaataattaa aatgttaact aaaaaatata
     1261 attaagtaga catttctatt aatctatgat acaatagtat ttgtggtaat acacaattaa
     1321 atcaagtata tcttgatata ggttaatagg agtattttta atgaataatg gaatagtgaa
     1381 atggtttaat aatgaaaaag gattcggttt tatatcaatg gaaggtagag atgatgtgtt
     1441 tgctcatttc tcagctatac aaacttcagg atttaaatca ttagaagaag gtcaacaagt
     1501 aagctttgat atagttaaag gtgctagagg tcctcaagca gaaaatataa ctatattata
     1561 atatatagat ttttcgggaa agactctttt tagagtcttt ttttattgtt aatatatgta
     1621 agataatcat tggtgaagta aataaggtat attgagtaat gttcttgata ttttatatgc
     1681 taatgaattt caaaaaaata agcactccta taaaaagagt gctcttaatg taaatataaa
     1741 ccttcattgg tccagcctaa taccccgtat tctccaagtt ctaactattt tttatataca
     1801 ttatcacttt catccatatc taacacaatt atttcttctt tagaatcaat ttgtaaaata
     1861 tgaggactgt gtgttgtgac aattatttgt gtttttagga gaattgtttt taatgtaata
     1921 aaaattttac ataccacata gttgatataa aaccccaaaa taaacttagc atctccaatg
     1981 tttacacata tacaatgctc ttaaaattgc agtgaacgcg caatagtgca attgataata
     2041 cttattatat attctcaatg ccttatattt caactgctaa acctcaattt gtcacaaata
     2101 tcgctcactg caaaatttct atatttttat tatatcataa catataatta atattttcaa
     2161 ttaattttta catttaatta tgtttaaatt ctataataat gcaactttaa tgtttctttc
     2221 tggaactaat ataaaaacta tttcagaaag attaggtcat gcagatataa aaataactat
     2281 gaatagctac tctcagtttt tagaagagat ggacagagaa gcatttgaaa atttgagcaa
     2341 aatactattt attatatttt aaagaagatg acttattcaa tatttgatgc tagaatataa
     2401 ttaataaaaa tttaattata ggtgattgta ttatgaatat tattaaaagc ataactttac
     2461 tcttaacatt attaattgtg atattaatga caggctgttc cccaagagaa agtgctagtg
     2521 aagttgcaaa aaaatatatt gataagatga actctggtga ctatgaaaca gcatatagtt
     2581 tattaagtaa agatagtaaa aaaaaaatta gtatagaaaa tcttgaagaa tttcaaaata
     2641 ttttagttag tacaaaaaga ataattagtt ataaaatagg taaagaaaaa atcataaaaa
     2701 aatataaaca tgatggaaaa gaatataaaa atgttgttaa attagaagaa acttttaata
     2761 taaaaaacct tctttataaa aaagatgatt ctctaaaagt aaatagatat tttgtagttg
     2821 aaaataacaa atacaagcta ttattgtatg aaagtttcaa aaaagaattt ggtattaata
     2881 gtatggacct tgctcaactt aagcttaatg attttggact aaaatacttt aaagaagttg
     2941 accttatact aaaaaaagct attgctatta atcctactga ttcaaagctt tattatgccc
     3001 aagcatgtaa ttatatgaat tttgatggat ttcctggtta ttcagataca acatataatg
     3061 ctttagattc aattaataaa tctttaaagt atttagataa aaatgattct gactacaaaa
     3121 aaaatgcaag taatatatat aacttaaaag gagttattct tatgtttcac aaaagatttg
     3181 atgaagcaca acaatattta aataaagcct tatactttaa caaagataat gaagacccaa
     3241 aatataatct aaatacaata aaaaaacatc taaactagat atttaatttt tgaatttata
     3301 aaatattttt ttatgtataa ttcagcactg aatcataatc tctactaaac taattacaag
     3361 tttaaattat aaagacttat ttctatataa atattactct ataacaataa gtttcttatt
     3421 taaattttaa aatattatct aaaaatcata aaaaagaagg tataaagagc taccctcaag
     3481 ctcaatatac cttataaata tctaaacttc attttttact aatttttcta tatctttgat
     3541 aatatgttct ttagattttt ttccagctat ggcatcaaaa attgcaacag cctttttctc
     3601 tccaaacttt tctgctacat cattaaagtc taaaaatcta acactttctt ttggtttacc
     3661 aaacatataa cacctctcat acatttttat atatttatta taacttctat agacttattt
     3721 ttcaccaata aacgttagac aaattagtcc ctttattttt ttaaactagc tttaccttca
     3781 aaattcttaa caaaaacagt ttattctact ctataaataa aaatagacaa tttaatttgt
     3841 aggaaactga taatagaata cagtaaataa aactttgttt aatctatctg aaaaagttat
     3901 ctattaaaac tgctatgaat ttataaagaa tatt
//
LOCUS       DACXMZ010000277         3933 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.278, whole genome shotgun sequence.
ACCESSION   DACXMZ010000277 DACXMZ010000000
VERSION     DACXMZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.278"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            136..1146
                     /locus_tag="JD016_17790"
     CDS             136..1146
                     /locus_tag="JD016_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001469.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            1282..1770
                     /locus_tag="JD016_17795"
     CDS             1282..1770
                     /locus_tag="JD016_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAU5001470.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            1946..3874
                     /locus_tag="JD016_17800"
     CDS             1946..3874
                     /locus_tag="JD016_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAU5001471.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
BASE COUNT         1521 a          387 c          724 g         1301 t
ORIGIN      
        1 attaatttat tttttaacat acaaatgatt ttatgaaaaa aaacaagact caaaaatttt
       61 tatattcttt gatagaataa aattgagagt aaatttattg aattttataa aatttataag
      121 gggtgagtct aagatatgca tgaagataaa aatgatatat ttgaatcaat agatactgag
      181 gattttgata ataaaacaga agaagaatgt ttagagctag taagaaaaaa tggattgaat
      241 ttaatataca taaaaaatca aacagaagag atatgtctag aggctgttag acaaaattgt
      301 aatgcaataa aatgtgttca gaatcagaca gaaaaaatat gtatagaagc agttaaacaa
      361 gattggagaa tgttagaatt tgtagaagag ccaacagaag aaatttgcat gttggctatt
      421 aaccaagatg gaacagcatt aaaatatata gaaaatcaga cagaggagtt atgcttaaga
      481 gccgttgaaa aaaatggtgc agctttggag tatgtaaaag aacaaacaga agaaatatgt
      541 atagaagcag ttagaaacag cgaatttgga ttggcaagag tgaaaaatca aacagaaaaa
      601 atatgtatgg aagcagttaa gcattgtagc tataatctaa aatgggtaga aaatcaaaca
      661 gaagaaatat gtatggaagc agtgagacaa aatgggcttg atttgaaatt tgtaaaaaat
      721 caaacagaaa caatctgttt aagagcagta agacaaaatg gaatggcatt agaatttgta
      781 aaggaacaga ctgttggtat atgcttaaag gcagtaagac aatatggaat ggcattaaaa
      841 tttgtaaaag aacaaacaga agaaatttgt acagaagcta taaaacaaga taaaagagca
      901 cttagttttg taaaaggaga taaggaaaaa tacaaggctc tatatgataa taatgaacca
      961 tttgcaaaga gatatgttag aaatgtaata gaaaaagaaa acagagcatt gattaaaaaa
     1021 ggagaagaaa atgcaaaaat caaaattgca ggaaaacttt atgatagagg catgtctttt
     1081 gaagatatat cagatatagt tgagattgaa ataagcaagt taaaagtgag tttaggtgtt
     1141 ttttaaagta ataaatgtag gtgtttttta tgggaaatag ttaataaaaa aataatcgac
     1201 tagctctttg taggacagca aaattagtga tataatttga tgtataataa attattttta
     1261 ttagataagg gggaattaaa gttgaagatt acagtattat accaaacaag aagtggaaac
     1321 acagaaaggg tcgctaagct gatagaagag ggcgctaaaa aggtggatgg agtagatgta
     1381 aagcttatga gacttgataa tattgattta gattatttaa atgaaagtaa agcagtaata
     1441 tttggaacac ctacttattt agcaaataca acttgggaag taaaaaaatg gtttgatgaa
     1501 gattcaaaaa aagttaattt agctggaaaa ttaggagctg tttttgctac atgtgattat
     1561 atatgtggag gtccagatgt tgcgatactt actatagtag gacatttgat ggttaaaggg
     1621 atgcttgtgt attctggagg tggttcttta ggaaagccat ttatacattt aggacatgta
     1681 cattcaattg aaggtccaga gttacaagat gagaaggcta taatatttgg tgaaagaatt
     1741 gctactaagg caaaagaact atttgcttaa tttctatatg catagtaatg attagagtaa
     1801 atttagggtt ttaatggaga tttgaagtaa aagttataaa ataaaaaatt ttccataaaa
     1861 ttactcaaga tgaattataa tataattata aataaattat attattaaaa tatttaaaat
     1921 caaacgtcaa tagcgaggga agtttatggt atggcatata gaatatgaga ttttttctgc
     1981 aattatagtt atttttttaa tggtatactt ttttagaagt aaatttatac ctactttaca
     2041 aaacaaaata tattgtgcac ttttaatatt ctcattttta tttataattt caaatatttt
     2101 aggttcattt tgtctaaata atatagacaa aattccaatt tttattactt ttttattgaa
     2161 tcagatatac ttattgttat tgccaatacc agcagcactt gtatcatttt atgttatggc
     2221 tattatttat caagatataa ggtatatgaa aaaaagatta ttatttttat ctataccact
     2281 tataatttgt ataatcttat ctattacaaa tccttttacg catattctat ttggattgtc
     2341 aaaagaaaca ggttatatac gaggttgtgg atatgttgca acttttgcat cttttttctt
     2401 ttatgcaata tattcagcat ttttatcttt taaatataga aaagcaatgc atgaatcaaa
     2461 aatatgggct attagaggtt ttcttatagt ttcaattgtg gcaattatct tacaggcaat
     2521 ttttttacag tatcttctta caggaactgc atgtgcatgt agtttacttt tgacatatct
     2581 ttctatgcag aatcgaggtc ttattattga tgaccttact ggtgtactca atagacaatc
     2641 ttttattcaa gagctagatt tgaatatcaa tactgaacaa tcaggattta ttattacaat
     2701 tgcgcttgat gattttaagt ttatgaatga aacttttgga acaaaaaatg gagatattgc
     2761 actaaaagaa gttggaaaat atttgattca gatacatgat gtaaaccatg tatatagaat
     2821 agggggagat atattttcta ttgttgtcaa tagtcaatta ggtatagaac cagatgatat
     2881 tatcagaaag atagaagaaa gatttaaaaa gccttggcta gtagaagaga taagttttaa
     2941 tttagcaact agtattgctg ttgtttatta cccagaaaat gcagaaacga cagaagatgt
     3001 aattgttgct atagatttct ctatacatga agcaaaacgt tctgagtcaa ggcgagttgt
     3061 ttatgcagat gcgtctataa gtgaaaagat aaagaagaaa catgttatta aaaattgttt
     3121 aaaagaagca ttaaaaaatg atgggtttga agtgtactac caacctatat tttctaatag
     3181 caggggcagg tttacttcag cagaggcatt acttaggcta gaacacaaag agttaggttt
     3241 tatacctcct gatgaattta ttccaattgc agaacaaaca ggattgatta actcaattgg
     3301 attagtagtc tttgagaagg tatgtagatt tatagctagt gatgagtttg aagatttaaa
     3361 attggataat atagctgtga atctttctgt agtacaatgt atgcaaaaaa acttagctga
     3421 tgatttactt tatcttatga ataaatataa tgtatcacca actaaattta aacttgaaat
     3481 tactgagact gtggctatgg gctcttttaa tataattaaa gaaacaatgg aaagattaat
     3541 tgatttaggt gttaagtttg cacttgatga ttttggaatt gcttattctg gagttactaa
     3601 tatgctttct ttaccatttt cagtaattaa gttagataag agtttgattt ggtctatgaa
     3661 tgaagatagt aggcataaac ttacagttga gacgattata gcacttataa ataaattgaa
     3721 tatgaaggct gtagcagaag gtgttgaaac cattaagtgt gctgaagatt taatagctat
     3781 ggagtgtgaa tatttgcaag ggtattattt ttctaaacca gttccagaag atgtttttaa
     3841 aaatttgttg aaagaaaata cttttacaag ttaacaccct ttattttttt aattacaact
     3901 ttgttcattg acactgccag cgatatatta taa
//
LOCUS       DACXMZ010000278         3903 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.279, whole genome shotgun sequence.
ACCESSION   DACXMZ010000278 DACXMZ010000000
VERSION     DACXMZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.279"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            51..923
                     /locus_tag="JD016_17805"
     CDS             51..923
                     /locus_tag="JD016_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001472.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            920..2209
                     /locus_tag="JD016_17810"
     CDS             920..2209
                     /locus_tag="JD016_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5001473.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2229..2783
                     /locus_tag="JD016_17815"
     CDS             2229..2783
                     /locus_tag="JD016_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5001474.1"
                     /translation="MLCMIPLIQGVANLDRISTAICLENYVAIIGIVMLVPVFSLEQS
                     KEIDEIVSSKALSQVIVQLIRILMSIIGIVVLIIGFVNVLIYLGCNFPVVEYIIGSFA
                     SAMFIGSLGMFFSNLFNNTIIGYMISIGYLILNMMTKGKYVGKFFIMSMTQGSFDEKY
                     WLILGSVVLIIASLLIKPIKRKIR"
     gene            complement(3050..3514)
                     /locus_tag="JD016_17820"
     CDS             complement(3050..3514)
                     /locus_tag="JD016_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU5001475.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFISLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPVVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
BASE COUNT         1460 a          467 c          593 g         1383 t
ORIGIN      
        1 atttataaaa ataaaattct ataaaattat ttctgggggt gtatttatat atggaaatga
       61 aaatagcagt atccaatctt agtaaaagat ttgggaaaaa acaagctctt gataatataa
      121 atcttgaaat cgacagtggt atgtttggtc ttttaggaag aaatggagca ggaaaaacca
      181 catttatgag aattttagcc actttattat caaaaacaga aggttcaata catatgtgtg
      241 gagttcctat tgagaatact aaggaaatta gaaatatggt tggttatttg ccacaagatt
      301 tttcaatgta tcctaacatg acagtatata aagcaatgga ttatttagca gttttatcgg
      361 atataccact aagaaaaaga agaaaaatta tagcaaagtt attaagcaaa gtaaacctta
      421 ctaattatca aaatgtgaaa gtaaaatctt tatctggagg tatgaataga aggcttggga
      481 tagcacaagc attaattcat aatcccagag ttttaatagt agatgagccg acagcaggtc
      541 ttgacccaga agaacgtata agatttagaa atttactatg cgaaattgca aatgatagaa
      601 tcgttatttt atctacacat attgttggag atatagaagc tacatgtgaa aacgtagcta
      661 tattgaatag tggaaaagtc atatacaatg gctcaacaga agcattagca aaactagctg
      721 aagggaaagt atatagtatt gaagtagata aaaaagacat tgaaaatata aaaagtagat
      781 ttatagttat aggaatgttg actcatggtg gaaaggctat acttaggatt atatcagatg
      841 ataaaccttt tgagacagca gtaaattgta atccaactat tgaagatggc tatatgctta
      901 taatgggagg agataatata tgaatctatt tataaaagaa tgtaaaaata tattaaagag
      961 tatggtatat tatattttta ttgtagtttt agtttttttc tatatttctc aacttggaag
     1021 ttcagtttca aatgatgtaa gtaaaaatat tcctaatgat ggaaatccat tagttgctcc
     1081 acaaaaagta gagaatattg aggaatatta ctctgtacac aatcaatatc catatggtca
     1141 aaaagaatct atgaaaacag aaaaggtcat tccaaatgca actataaacc taattagaga
     1201 atatcaaaaa aacacatata caacgtatcc aacaggtgta gttaagtata taaaattaga
     1261 ccaaaataaa acgaagacca tagaaaaata tatagaagaa ataactggta aaagtattag
     1321 cgaaataaat tcactttgga aaactgatga aagtataaaa atagattatt ctaaagtatc
     1381 tcttgaaaga tttgaagaaa taatgcaaag agtagacgac attttaggag gagggtctgc
     1441 atatgctttt gacaagttac aaagatttgg agtagttcca gttacctatg atgaagctat
     1501 gaagaattac aactatacaa tacaaaaaga taaagttagt aatgggtatg caagaatttt
     1561 ttgcgattat ataggtatag ttttaagtat atttccagta tttgtggcag tttttatgtc
     1621 acttaaagat aagcgttcaa gaatgagaga gattatttat tcaaaacaag tatcttcatg
     1681 caaaatagtt ttatcaagat attttgcact tgtctttatg atgatattgc ctgtattaat
     1741 tttaggtatg aaagatttac ttcctttgat tttctttgct aaagataaag gtatatctat
     1801 tgataactta gcatttataa aatatacatt atggtggata ctaccaactc ttatgatagt
     1861 tacagctgta ggaatgagta ttactatatt aacagataca ccagtagcaa ttattgtgca
     1921 attaatttgg tggctttaca gtataaattc tacaggactc aaaggtgatt attcaacaat
     1981 gggactaatg ataagacata atagtgtatt aaatggggat attattatac aaaacttcaa
     2041 tcaaattatg attaatagaa ttgtgataag cttgggagca ttggtgcttg ttttgttagc
     2101 tatattctta tatgagcaaa aaagaagggg gaatcttgat gtttgggcaa agttttcaaa
     2161 actgtttagt tctaataaaa gaaaacttca aattaagcac tttaactaat ttaatatgtt
     2221 caattatatt gctatgtatg attcctctaa tacaaggagt agctaattta gacagaattt
     2281 caacagctat atgtttagaa aattatgttg caataatagg gatagtaatg ttggtacccg
     2341 tattttcgct agagcaatca aaagaaattg atgagatagt atcttcaaaa gcattatctc
     2401 aagttattgt acaattaatt aggatactga tgtcaattat aggtatagtg gtattaatca
     2461 taggttttgt aaatgtttta atatatcttg gatgtaattt tccagtagtt gagtatatta
     2521 ttggaagttt cgcatcagct atgtttatag gaagtttagg aatgtttttc tcaaatttat
     2581 ttaataatac aatcataggt tatatgatat ctataggata tttaatttta aatatgatga
     2641 ctaaaggcaa gtatgttgga aaatttttta taatgtctat gactcaaggt agttttgatg
     2701 agaaatattg gctgatactt ggaagtgttg tgttgataat tgcatctctc ttaataaaac
     2761 caataaaaag gaagattaga taataaaatg agagcttaga taaaataaag ttaatacaaa
     2821 taaaaagtac atttatgaaa tataaatcta tttttatata cacaaaccta cttacaatct
     2881 ctttatatat gtagatagat ttatatatcc gtagtataaa atatatatgt atacaaaatt
     2941 ttatcaaaag ctctcaaatt tatatttcaa agacacttat tgctatggcc tacacatatg
     3001 tattttttca ttttaattgt tgtattaaca ataagactat tgttatagac tattgatacc
     3061 tcataacaaa aaattctcca tctcctaaat gcgttttctg taccttagtc tcaactattt
     3121 taaatccttg agactcataa cattttacag ctctcatgtt ccatgttctt acatttaata
     3181 caactggttt agtactatat ttatttctac attcttgtaa tcctaggcca attatttctt
     3241 taccaattcc cattccgcaa taattcggtt tcataccaat accaaagaat acagagcttc
     3301 cttcatctaa taagcttata aaacctatca attctttatt gtcatttata aaacttataa
     3361 aattttttct tttatcttct tttgctagtg aaaaattctt ctttttaatt tcctcccatg
     3421 gaggtagatt gtatattgaa tatttacctt catatttcca attagtaact tcaatagcat
     3481 gttcttcagt aaatttaact ttatttaaat tcatatgtcc tcctattaaa atatttaagg
     3541 taattaatgg tattttaaag ttttagaaaa taagtaaaac tatttatatt tatccataaa
     3601 atctttaaaa attttactaa caattcttgt accctgtact ttttattgga ttcacagaag
     3661 actataaact aattttgaat taccttctaa ttcaaaaaca tcacgatata tataatttct
     3721 tagcttgatt aaattctgtt aaatccgttt cttatagttg tataatcaac atcaatatct
     3781 ttatgtgtat tagttaattt attggcttta ggaacttatt attaatatat tgtatttact
     3841 ccaagtagta ggtaaattat aggcaaatta tcaaatgtat atctatccat gcttaaactt
     3901 tag
//
LOCUS       DACXMZ010000279         3867 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.280, whole genome shotgun sequence.
ACCESSION   DACXMZ010000279 DACXMZ010000000
VERSION     DACXMZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.280"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            124..1779
                     /gene="ilvD"
                     /locus_tag="JD016_17825"
     CDS             124..1779
                     /gene="ilvD"
                     /locus_tag="JD016_17825"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAU5001476.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELETLAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRMAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIGNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGIVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            1904..2026
                     /locus_tag="JD016_17830"
     CDS             1904..2026
                     /locus_tag="JD016_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001477.1"
                     /translation="MKTIHLYYKEYEYSIEMYLLDLVEVIQLFKSLMWNAFRSI"
     gene            2123..2752
                     /locus_tag="JD016_17835"
     CDS             2123..2752
                     /locus_tag="JD016_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAU5001478.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTFTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            2778..3590
                     /locus_tag="JD016_17840"
     CDS             2778..3590
                     /locus_tag="JD016_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAU5001479.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELSKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1496 a          443 c          730 g         1198 t
ORIGIN      
        1 aaatcgtaat aaattttcat tagtatatgc taatattttt ttacaatttt cgtaattaat
       61 taatagaaaa atatattata ataatattat gaaaatttaa aaaagtaaga aagaggtaat
      121 attatgagaa gtgatataaa aaaaggaata gaaggagctc caaagagagc gttaatgtat
      181 ggaatgggtc ttactaaaga agaaattgaa agaccattaa taggtatagt aaatgcacaa
      241 aatgaggtga ttcctggtca tctgcatctt gatgaaatag cagaagctgc aaaaaatgga
      301 gtaagaatga gtggtggttt gccattagaa tttcctgcta taggggtatg tgatggtata
      361 gctatggggc atgttggaat gaattattcc ctggcttcaa gagaattaat agcagattca
      421 atagaagcta tggctatggc acatggtttt gatgctttgg tacttatacc taattgtgac
      481 aagatagtgc caggtatgtt aatggctgct gcaagactta atataccaag tattgttgtg
      541 agtggagggc ctatgcttcc aggtaaaaaa aatggaaaag tatatgattt taactctgca
      601 atggagggtg ttggagcatg taaagatgga acagtaagtg aagaagaatt agaaacgtta
      661 gccatgaact cttgtcctgg atgtggttca tgttctggtc ttttcacagc aaatagcatg
      721 aattgtttaa cagaagcttt aggcatgggt ataccatata atggtactgc tgcgtcacat
      781 tctggagaaa gaaaaaggat ggcaaaatat gcaggtatgt atgttatgga gttacttaag
      841 aacgacataa aacctagaga tattttaaca atagatgctt ttaaaaatgc tatagctgtt
      901 gatatggcaa tggctggttc tacaaataca gtacttcact tacctgcaat agcttatgaa
      961 tcaggaatag agcttaactt agattttttt gatgaaataa gtgaaaaaac tccttgttta
     1021 acaaaattaa gtccaagtgg aaaacatcat attgaagatt tacatatggc aggaggaata
     1081 ccagctataa tgaacgaact ttcaaagata aatggaataa atttagattg caaaaccgta
     1141 acaggcaaga ctatagggga aaatatagga aattgtgaaa tagaaaatga agaagtaata
     1201 catacattaa agaatccata tagtaaccaa ggtgggcttg caatattgaa aggaaatctt
     1261 gctctaaatg gagctgttgt aaaaaagtca gcagttgcag aagaaatgtt agttcatgaa
     1321 ggaccagcaa gagtttttaa ttcagaagaa gaagctgtaa atgctatttt tggtaaaaaa
     1381 ataaacaaag gtgatgttat agttataaga tatgaaggtc caaagggtgg tccaggaatg
     1441 aaagaaatgc tatctcctac atcagcagtt gcaggaatgg gacttgataa gcatgtagca
     1501 cttcttactg atggtcgttt ttcaggggca actagaggag catccatagg ccatatttct
     1561 ccagaagcta tggaaggtgg tttaatcgga atagttgaag aaggagatat aatttctata
     1621 aacataccag acaaaaaatt agagttaaaa gtagatgaag ttgaaataga aaatagaaaa
     1681 ttaaaattta aacctttaga accgaaaata aagcatggat acttaagtag atatgctaaa
     1741 ttggtaacat cagcaaatac aggagcagtt ttaaaatagt agactatatt tatgtaaatt
     1801 agaaaaagtt aagaaaatat taattggata tcttaggtgt tatagcacta atgaggtatc
     1861 ctttttattt tgtatgaaaa acacagcaaa atcttgatta tctttgaaaa caatacatct
     1921 ctattataag gaatacgagt acagcataga gatgtatctt ttagaccttg ttgaggtaat
     1981 acaacttttt aaaagtttga tgtggaatgc ttttcgttcc atctgaacta tgaagaaatt
     2041 gaaagtcagt taattcttga cataataaat attaaaatgt ataataaaaa acaaacagac
     2101 agtctgtttt taggagggaa atatgaatag agaagaaaaa agtaaaaata gtaaagaaaa
     2161 aattatccaa tcagcatttt cactattttc gtctaaagga tatgattcaa catctacaca
     2221 agatattatc aatttatctg gtttatctag aggtgcaatg tatcatcact ttaaaactaa
     2281 agaagatata ctgagaagtg tcacaaaaga actttactca caaatgaata attttttaga
     2341 gcatcttgtt gctgatgaca cctttacagc aaatgaaaaa ataatagaat tggttgttca
     2401 tagtgcgaat gattacacac gtagaaaaat ggtacattgt agctggttag aaaaaatccc
     2461 attcgcttta atagaggaag ttcgtaatct caacaatgta gttgcaccca atattgctaa
     2521 gataattaaa caaggtgttg aaaataaaga attttcttgt gaatatccag aggaattagc
     2581 tgaaatgctc gtttttagta ttgatatttt acttgaccct gtattattta aacgtgaata
     2641 cagtgaagta tgtaataggt tggatttttt attattcatg ttaaaaaaaa tggatattcc
     2701 tctaattgat gagtacggta ttcaaaaatt taaagattta tttaaacaat gataatagga
     2761 aaagtgagga atagactatg ttttataatg caaaaagctg tcaaatgatg ataggaagta
     2821 atacagtgaa ttatattgaa tttggtagtg gtaagaagac actaattatt ttaccaggat
     2881 taggagatgg tttatttcct ttacatggaa aaatacaggc gattgctttt gcttttagat
     2941 ataagcaatt tgcaaaagat tataaggttt atgtatttag tagaaaaaat caaattacag
     3001 aaaaatattc cacaagggat atggctaaag accaagcaga tataatgaaa aaacttggaa
     3061 ttatgaaagc agaagtaatg ggtgtttctc aaggtggaat gatagctcaa tatctggcaa
     3121 ttgattatcc tgagttagtt gagaaacttg tgttggcagt tacttcttca aagcaaaatg
     3181 atactatcca aaatgttatt tgtagctgga ttgatatggc gaagaagcag aattacaatg
     3241 atttaatgat tgacacagca aagaaatcct attctgaaag atatttaaaa aaataccaat
     3301 tatttattcc ttttttagga aaagtaggta aaccaaagga ttttaaacga tttattattc
     3361 aagcgacctc ttgcatagaa cataatgcct tttccgaatt aagtaaaata acatgtccaa
     3421 ccttaattat aggaggtgct aatgataaaa ttgttggaaa taatgcttct tttcatttag
     3481 cagaaaagat taaaaaaagc gaaatattta tctatgaggg attgggacac gctacatatg
     3541 aagaagcaca ggattttaat gaaagagttt tagagttttt aaataagtaa acacctttgt
     3601 taaaaatata catatgaaga ttggaaattt aatgttaaaa atagaaacat gaaaatatgc
     3661 ttaactggta tttttactat tcataaccaa tttttgatac attatctact ataaatacaa
     3721 atatagcttc aatgtgatta tatattagtg tattggtaaa gcacttatac aaacagagga
     3781 attttgtaaa ttcatattat acccacattt gttaacttat gaaaatataa tcaaaatttt
     3841 tatgagattt ttatataaaa aatataa
//
LOCUS       DACXMZ010000280         3859 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.281, whole genome shotgun sequence.
ACCESSION   DACXMZ010000280 DACXMZ010000000
VERSION     DACXMZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.281"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            29..775
                     /locus_tag="JD016_17845"
     CDS             29..775
                     /locus_tag="JD016_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001480.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            775..2691
                     /locus_tag="JD016_17850"
     CDS             775..2691
                     /locus_tag="JD016_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5001481.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNKNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            2970..3203
                     /locus_tag="JD016_17855"
     CDS             2970..3203
                     /locus_tag="JD016_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAU5001482.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSIFISTKW"
     gene            3399..3827
                     /locus_tag="JD016_17860"
     CDS             3399..3827
                     /locus_tag="JD016_17860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAU5001483.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
BASE COUNT         1556 a          382 c          610 g         1311 t
ORIGIN      
        1 aaattaaatt aggaaggata gttaaataat gaaaatttta actgtaaata atttaagtaa
       61 agtatatggt aaaaaaataa ttttcaacgc attaaatgac ataaatttta gtatagaaga
      121 tggagaattt gtaggaatta tgggtccatc tggtagtgga aaaactactc ttttaaatat
      181 gatttctact attgacaagc caactacagg aactatggaa ttaaagggca agaatccttt
      241 attgttaaga ggtgaggaac ttgcattatt tagaagaaga gagttaggtt ttgtatttca
      301 ggattttaat ttattggata cattaactat aggagaaaat atagtattgc cattgacatt
      361 agataaggtc agtgtaaaag agcaagatga gagattgaat gaagtatcta caatattagg
      421 tataaaagat ttattaggaa aaagaacttt tgaagtatca ggtggtcaag ctcagaggac
      481 tgctattgct agagcattga ttaacaatcc atcaatactt ttagcagatg aaccaacagg
      541 taatttagat tcaaagtcat caaaggttgt aatggagcta tttcaaaaga taaataaaga
      601 aaataaagtt actactatga tggtaactca tgacccatta gcagcgagct actgtagtag
      661 aatcttattc ataaaagatg ggtctatata taatgaaata tataagggta gtagtagaga
      721 gcagttttat caagagataa tggatgtact tacattattg ggaggcgata actaatgaat
      781 ataaaacagt ttgctattaa caatatttca agaaatatta aagcgtattt aggatactta
      841 gcaagtattg ttatatcatc ttcactgcta ttttctttta ttatgtttac aagtcatcca
      901 gatttagatg tatctcaatt tcctgattat ttaaaagaag gtctcaagat gagtaagata
      961 attgcatatc tattcttatt cttctttgtg ttttattcag ttagtgtgtt tcttaaaagt
     1021 agatataaag aatttggaat actttatatt acaggtatat caaagcgcca agtaatgaaa
     1081 ttaatcttta tagaaaatat gataataaat atagtatctt ctgtaattgg aataataata
     1141 ggcttaatct ttgcaaagat atttttagtg gttatgtcta cttttttaga attatcacca
     1201 ttaaaattct atataccatt taatgcgata ttgtctactt taatctattt tatgatttta
     1261 gctgtattaa catcaatatt tacttctttt atagttaaag aaaatcaggt gttgaagtta
     1321 cttaaaggta caaaaactcc aaaaccagag cctaaaacat cactaatgct tgctatatta
     1381 agtatttgtt tgtttataat agcgtattat aatgctatta cctcaaccga ccaatataca
     1441 acatatctgc gcttagttcc agttacatcc cttactatag tagcaactta cttgttcttt
     1501 tctcaattta gtgtattttt tatgaagaag ctaaaattaa acaagagatt ttatagaaaa
     1561 aatacaaata tgctttggat ttcgaacttg atttataagg taaaggataa tgctagaata
     1621 tttttcttga taactataac ttctgcagtg gcatttactg ctataggtac agtttattcc
     1681 ttttggaaag atgtagagcg tcaaattaat ttaatatatc ctaacacaat atattattca
     1741 acaatgacat tgcataatga ccctaaaaag cctgatagca aagaaaaaga caaggaaaga
     1801 atgatgttta tagaaaataa tctaaaaaaa gagaatgtag actatacaag gattaatggt
     1861 gaatttaaaa ctgtatttcc caagaaaagt gattttaatg tcagaattat gaaagaatct
     1921 aagtacttag aaattacaga aactataggg gttaagccta taagttttga agataatgaa
     1981 tgtatatcat tattatcatc aagtttacca ggaaataaga acgtcaaaga aaatattatt
     2041 gtagggaata gaggtttaaa ggtggcaaaa caagtggaag aatgtgttat gccagcatat
     2101 tacaaaaata tgtatgtggt aaaggataat ttttatgata gtatagaatc aaaatttata
     2161 gtggatagat ttactgcatt tgaagtagag gattcaagta aaactataga gatatgcaga
     2221 gaatttgaaa ataaatttaa taatgagtca gggatgcaac catatttgtt tttctctaaa
     2281 gaattaatga taggaactgg gaaattaatg tattctacat ttatattttt agcaatattt
     2341 ataggtttaa ttttctttgt tactacaagt agttttttat ataataaatt ttatatggat
     2401 tgccaagttg ataagaaaaa gtatgaacag ttaaataaat tgggtatgac atataatgag
     2461 attaaaaaag cttcaacaat tgagattgga atagtatttt tgtttcctta tgtagtggct
     2521 gttatacatt caatatttgc acttactgca cttaaaaatt cttttgatat agaagtagca
     2581 tcatcagctg ttttggtaat gggaagcttg ttcttggttc aaatagttta ttttttaatt
     2641 gtaagaaata attatttaaa agagataagg ttaaatttag tcaattttta gttactatta
     2701 ttaaatgata ataaaaaggg tttagaatta attaatatta ctggaattat agatgtttat
     2761 tttttcagta atatttttta ttgtattaac tattaaaatt ttgtacaaat ataaaatata
     2821 atatggtaaa tgtcaaaatt ataaaaaaat tctacttata taaaatataa agaggttata
     2881 tgtacatatg tgtagaaata aataataaaa atatcaatta atgttattag ggggggttac
     2941 tcattagtaa attagctaat acacttagaa tgatgatatt aattcaaagc agaggcaaaa
     3001 tgaaatgcaa agaattatca gaagagttgg aagtaagtga aaggcaaata aaaagttaca
     3061 agatgtattt agagcaagct ggtatattta taaattcaac acctgggatt tatggtggtt
     3121 atgaaataga taaatgtaat agcatatcat taattaaatt attagatagt gaagtttcaa
     3181 tttttatatc aactaagtgg tagataagta gaatattagc aatatcaacg agtatttaga
     3241 aacttatttt atattaataa agcagtatat aaaattgtag aatcaacaat agcatatact
     3301 aaaacatcca ccttatatta actaaatcat atctaaataa aatacactta cctacaaaca
     3361 ttataaaatc aatacaaaaa tgaggtgaaa caaaatttat gataaagaaa ttaaacaata
     3421 aagatataaa taaaatcatg gaaatatggg aaaaaagtac aatcaaagca catgacttta
     3481 taagtaaaga atactggcaa aataactata atactgttaa aaacgaatat atacctatat
     3541 cagatacatt tgtatatgat gatggagatg aaataaaagg atttataagc ataatagata
     3601 aaagctttat aggagcttta tttataaaac ccaaatacca aaatctaggt atcggaggta
     3661 aacttttaga ttatgcaact aaaaaatata aaagtctaag cttagcagta tataaagata
     3721 ataaaaaagc agttgtgttt tataataaaa aaggttttaa tatagtaaaa gaacaagtaa
     3781 atgaagattc aggatttaaa gagtacataa tggaatatag taaataatat gattacattc
     3841 tattaaaagg ataattaat
//
LOCUS       DACXMZ010000281         3857 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.282, whole genome shotgun sequence.
ACCESSION   DACXMZ010000281 DACXMZ010000000
VERSION     DACXMZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.282"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            85..759
                     /gene="fsa"
                     /locus_tag="JD016_17865"
     CDS             85..759
                     /gene="fsa"
                     /locus_tag="JD016_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAU5001484.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(881..2275)
                     /locus_tag="JD016_17870"
     CDS             complement(881..2275)
                     /locus_tag="JD016_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAU5001485.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(2353..3699)
                     /locus_tag="JD016_17875"
     CDS             complement(2353..3699)
                     /locus_tag="JD016_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAU5001486.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNTVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
BASE COUNT         1528 a          531 c          531 g         1267 t
ORIGIN      
        1 ataaaaaaca ataagcaaaa tgttgaaaat aataccaaat caatatataa taagaataaa
       61 gaaagattaa agggggatat tttaatgcaa ctaatcttag atacaggtaa tgtggaggag
      121 attaaggagt tatgtacttg tttaccaata gatggtgtaa ctacaaatcc aagtattgtt
      181 tcaaaagaaa aaaagaattt taagcaactt ataaatgaaa ttggtgagat tataggtgag
      241 gatatgccta tacatgctca agtgttaagt acaaagtatg aagaaatatt agaagaagca
      301 ttatatataa gtagtttgag aaaaaatata tatgtaaaaa tacctgtaac acaagatggt
      361 ttaagagcta taaaagacct tcatagaaaa ggtgtaaaaa taactgctac tgcaatattc
      421 actgctcatc aaggtttttt agctgcaaag gctggagcaa attatattgc accttatgta
      481 aataggcttg ataatatatc tggtgatgga gtagctatgg tttcagaact tataaaaata
      541 atagatactt ataaaatgga tacaaaagta cttgcagcca gttttaaaaa tgcacaacaa
      601 gttatagaat taatgcaaca tggggtgcat agtgttacag taccatatga tatttgtaaa
      661 tctatgatga atcatccatt aactgattgg agtgtagata agtttataga ggattgggag
      721 aatacttttg gaaaaggaag taaaacaaat aatatttagt attatggtaa aagagtagtt
      781 ttgtccaatt ttacaagaac ttatataaaa agatgaaaat atgaattttc tttttaataa
      841 tttcattatt atcttaagtt aattcatatt ttcatctttt ttagatttct agtcctataa
      901 atttccacca agggaatgtt atacatatta ttataaagta ggttaaagga atcaaagtta
      961 aaccgtattt gaaaataacc ttgctattat acagattttc accatgacca accattattg
     1021 tagcatgatg aaatggtaat atgtactgta tgcttactat aatgtagaca aatagacata
     1081 aagtaacact attttccttt agtatatgta aacttccaag taatggtatt actacagatg
     1141 atgtagtgac agcacttcca agacatatat tcaagattat tgtgagagtt atcaaaaaca
     1201 atattgaaat aatttctgaa gagtttggta taaactttat caaataattt cccattatat
     1261 ctacaactcc agagtacttt aacactcctc caattgaaaa tgctgctata aaaaaaataa
     1321 ggaccttcca atttatttct tttaaagtac taaatccaac tatttttctt agtaataaaa
     1381 gaagtacaca aattgacatt ataattaatg tgtttatgtt atgtatgaac tgagttaaaa
     1441 acataaaaaa cattattcct aggcataaaa atacgtgttt ttgctctttg tttagcaaat
     1501 taaattcttt ttttaaatta gaatatgaaa aatgaagttt attaccctca agattttcat
     1561 tagaaataaa ttttattgaa tgcaattctt ttttaaagac aaagataaat aaaaaataag
     1621 ttataaaaca agtaattata gttggaacag acatataaaa agcccattga ccccaattta
     1681 tattacaatt tccaagttct agtacaacat aatttagaag agtgtctcca tttacaaaaa
     1741 acattgatgt aaaggtagat gctgtaaata tactaaataa aagtatagat tttgaatctt
     1801 cagttatctg ttgttctttt aaaaactcct tgtaaaaagc agatacaaga ataacccttg
     1861 gaaatggttg tgggataaaa aatattaaaa gtatacctaa aatatacgat aaaagaatca
     1921 tttttttagg ggtatttaca gtatttaaaa ttaacttctc agaaataatt tttgcgacac
     1981 ctgtttttgt aactgcatta gtaattatgt atgcaagaat tattatgtaa aaattttctg
     2041 taaataaaaa ctgtaaaata acctttattg gtgcaatgtt aaatataaac gctagaatta
     2101 caaaaaacaa tgcaacaaaa gttttttcaa ctgagtctac agcccatatc aagatagtta
     2161 aaaaagataa gcatatagaa aattttatgt tgaaactgta attaagagag aagtagagaa
     2221 ttatggtagc tataacagtc atataaaatt gttttggctt aagaataata cccattttta
     2281 tattcacctc cataatttat tatatcataa tggaggtgaa tacaatgaat gtgaatacat
     2341 tttataaaaa gattattcat tgattctatg atttacaata gctttagttt tccaatgtcc
     2401 tttcttatag tagataactg taacaataag acctaaagtc caaccaaaag gaatacccat
     2461 ccaaattccg tctgttccaa aaaatttaga aagcatataa gaagctggaa gtcttccaag
     2521 ccataaagat actattgagc ttataagtgc aaacatggaa tcaccagcac ctttcattgc
     2581 acttgaaagt acaaaagaag ttccaataaa taagaagaat ggtcctatta taaataagta
     2641 agaagagcca attttaatga catctttatc agtattgaat agagcaatta gattttccct
     2701 aaatagcaaa attaaagcaa taactgtaac tgctaaaact aaggtcattt ttatagattc
     2761 tcgaatgcct tttttaactc tttcattttc tccagcacct atgttttgag caacaaaagt
     2821 agaaacagca gaaccaagat tcataatagg taagcttata aaattttcta tcctaagacc
     2881 agcaccaaaa gctgccatag cagatgtacc ataactatta actaaaaatt gaagagccat
     2941 atttccaatt gaaaacaaca tttgttgaac tccacttgga agtcctaatc taagtccatc
     3001 tttaaatatt ttgttatcat atacaattcc tttgatttta aatttaagca cttcatgttt
     3061 tttatttaaa taaattatac ttattataaa tgatactgcc tgagccatta tagtggcaag
     3121 tgcaacgccc tcaacaccca ttcttagcac tacaataaaa gttaagtcta aaactgtatt
     3181 tagaattgtg gctattatca gaaaataaag tggagttact gaatctccaa gtcctctaag
     3241 tattgcactt actgaattat atccagcaga aaataatatt ccactaaata ttatagtaag
     3301 atacaatttt gattgagcaa aaacggattc tggtgtattc ataagcttaa gcatggggcc
     3361 accaaatact attccaagaa tacttattaa tatagaacca ataaataaaa gtatatatgt
     3421 agtatcaata gtagctttta ccttttttaa atctcctgag ccataaaatt gtgacactaa
     3481 aatagaaaat ccaaggctaa cacccataag cgctgccacc aataaaaaca ttatagggtt
     3541 agctccagag actgccgcca ttgcttcttt tccgataaat ctaccaacta ttatactgtc
     3601 agctgtattg tatagttgtt gaaataaact accaataagc ataggcatgg caaaaaagaa
     3661 tatggacttt ccctcatgac ctgtagttaa atctttcacc ttattcctcc aaataaaaaa
     3721 attattaatt tattatagta taatcttaac ataaacttta tatccaaact ataaataaat
     3781 tgtatcaaaa tatcatatat ttttttaaaa tgtaaagaat ataattataa tttgaattta
     3841 ataaatcaat atttaat
//
LOCUS       DACXMZ010000282         3853 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.283, whole genome shotgun sequence.
ACCESSION   DACXMZ010000282 DACXMZ010000000
VERSION     DACXMZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.283"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(144..539)
                     /locus_tag="JD016_17880"
     CDS             complement(144..539)
                     /locus_tag="JD016_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5-phosphate oxidase"
                     /protein_id="HAU5001487.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            680..1072
                     /locus_tag="JD016_17885"
     CDS             680..1072
                     /locus_tag="JD016_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5001488.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(1172..1588)
                     /locus_tag="JD016_17890"
     CDS             complement(1172..1588)
                     /locus_tag="JD016_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HAU5001489.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(1603..2514)
                     /locus_tag="JD016_17895"
     CDS             complement(1603..2514)
                     /locus_tag="JD016_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAU5001490.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPVKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(2696..3718)
                     /gene="splB"
                     /locus_tag="JD016_17900"
     CDS             complement(2696..3718)
                     /gene="splB"
                     /locus_tag="JD016_17900"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HAU5001491.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
BASE COUNT         1293 a          657 c          429 g         1474 t
ORIGIN      
        1 attattgaaa tacacatcta ataactaaaa atttttacat attcattatt taaaaatctt
       61 tttattgcat ttaaattaaa tctatcaata tttcagtaat aacgttttct taattttcac
      121 tatggatgtc gattcaatta taattaaagc tctacatcaa tctttttacc actcattgaa
      181 caacaactaa cttttgcctt atcaatagaa aatatttcaa acttaccatc tcctacagag
      241 tacatctgac ctaaacttgg cattatttca agtgccttag cttgtgtttc ttttgatgta
      301 acaaatactg cttcacctaa aatacgtaga gtattaaggt tttggtctat acaacaaagt
      361 tcaacctttg gattaaaaat aagttgctta aacatatcct tttgattaga tgtgcaaaat
      421 gtaagtttac cattgtattc cataacaaat cctaaaggac gcacatgtgc ttgattgttc
      481 tcatcaactg ttgctaaata ataaactcct gccttgttca aaaattctaa tgctttcata
      541 attcttacac cccttttaaa tacttgtatt aaggattatc ctttgatata attttaatat
      601 aaaatacaga aaaaacaagt atgcagtttt ttctgataaa gtataaaaaa gatactacta
      661 taactaagga gtaattaaaa tgagtataaa tactgaaaaa aatataacta aaaacgttga
      721 agaattaacc tgtccaatcc gttatgcatt agacattgtc ggtggtaagt ggaaattacc
      781 tattatatgt atgttagcag ttgaaaatcc aattagatat agtagcataa aaagaaaatt
      841 ggatggcatt acaaatacta tgctagctca atcattaaaa gatttagagt ctactggtat
      901 tgttcatcgt aaacaatata atgaaattcc tccaaaagtt gaatatacat taacaagtaa
      961 aggtaaaagt attgtgccta ttttacaaca atttgctaat tggggtgcta ctaatatgca
     1021 agaaaaaaac acatgtggac ttagttgcaa agaatgtaga aagataaagt aaatttaaat
     1081 ttactaaatt attcatatta tagactatat attaatttat ctgattgaat ataaaaacag
     1141 gatgacttat atattcatcc tgttctatat gttaagctct aaatttaatt agttcaagca
     1201 tatcgtttaa taccaataaa ctatctactt gaatcattag ccatttactt ccattaaaaa
     1261 agtttacctc attatatatt tttttgatat aatcacaaca agtattaata aataaatcaa
     1321 cctctactct attgctttca cttacaataa ccaaagcttt aaaataacct tcttgtggat
     1381 ataaagtaca tagtgattta cctctttttt tatattttac attccaacct ctttgcattg
     1441 aacatttact gtattcaagc ttaggttcta cattataagt agaaaccaaa gttttattta
     1501 acttgtccca taatggatta ttaataaatt cattaatttg ctcaaatgta ggtttagtat
     1561 cttgaaaaaa cgcatcttgc caattcatat tcatcatcct ttctattgtt caataggaac
     1621 cggaacaatc caatgttcca gatatgctgc ttcaggtgta gtggggtaat acatctcaat
     1681 tgcaaaatca gtagttgtta aattatgttt cttcatccat ctttccataa atttatctgc
     1741 tttaaaaaca gctgaattaa ctaactcatt aaagttttct gcctcaaatc cacatactaa
     1801 atattcttta actggcaact caaaagtact atatccttct actgatgtac ctgactctac
     1861 ctctacacct gccatataca tacaatttcc ttcttttgca tttcccatat aaagagcagc
     1921 aaattcattt ccacctggaa tttgatttgg tatattatgt ctttgacttc caagtttcat
     1981 ccataattcc tctactattg caattccagt tgtttcaccg cctaccaact ggcaaatagg
     2041 tacttctatt tctattccaa caaatgtcct tggattactc aatgtcttac gattaacttc
     2101 aatcactata tcatctgcaa tcagtggaac accttcatca accatagcat aatttaaaat
     2161 taattctggc ttaataaagt gattgagttt tactggattt gctctatatt tttctggagt
     2221 taatccatat gcttttttaa aggaacgtgt aaatgtctca tgactagcaa atccaaaatt
     2281 taaagctaca tcaagtatac gattcttatg ttctgctaag tattcagaag ctcttgcaag
     2341 acgacgaagc tttatatact ccattacagt cttatttacc aatcttttaa atagtctttg
     2401 atagtaaaat ggtgataaat ttgctttatt tgctaattca ttaattgata tttcttctga
     2461 taaatttgct tctattaaat ccaatgtaat ttgaatagac tcccatgcat gcatactata
     2521 actcctcctg atatttttgt tactcttatc ctttatagta tcaaaactag aaaaaatgtt
     2581 cttgatagat agtgcccttt ttaattttct tatatgatag tgctaaatct cattgcttta
     2641 ttataaacat aaaatatata aaaataaaat atagcaaatc tttaggtaag atttgctata
     2701 ttatatattt tattaaacta tttttaaata ctccatttat ttccttaaga aagaactctt
     2761 tcatatcatc taattgttct ttatcataca cgtacttacc atatccaaat tgaccgtatt
     2821 taaactttct atcttcattt tccattggca atagtgtatc tggataaatc tctagtattc
     2881 tattttttgc tttagttgta tatctatgag atataacttc aaatataatt tttttatcaa
     2941 aatcctttgg taaagcttct tttatactta taatcagatt tttatactct tctttccaac
     3001 cttcatataa aaacacaggt gctattatga aaccaatctg ataaccagcc ctagcaagtt
     3061 ttacagctga ctctattctt ttatttgcag aggctgtata atgctcatgt tcatttataa
     3121 ttctaggagt attgatacta aatcgtattt ctgtatgatt attatgttca gcatccaata
     3181 agctatccac atcactgtat ttagtaacaa atctaaatct tcctttttcc tctcttccaa
     3241 aaaactctat tgttttcttc aaagaattag tatatggctc tactggaact gggtctgatg
     3301 tagctgcccc ttcaaatatg gttacctgtg gctttctatc ttcaatatat ttttttgtct
     3361 gttcaagtat ttcatctaca tttacaaaca cccttataaa tggtttatca cctaattgtg
     3421 tgttcaaata acaatactca catcttccca tacatccact gacaagtggt agttgataat
     3481 tagcagaagg tttacatgtt tgaaacttca gactttttct aatcccaact accatagtct
     3541 gcttcccact tctatattgt tcatatagat tttcacctgg aatatgttct ttaatcctat
     3601 ttgatgatat attaattatc tctacatctt tattatctct aaatttattg tatatttttt
     3661 tacccacatc agtgtctaaa gttccttttt cgaatattat tcttttagga ttaaacattt
     3721 atttcacctc tacttaaagt ttttaaatcg aagaatttta taaaatatta cttaattaat
     3781 cttccctttt ttaaactcct ttatttatta gaaagtaaaa taaaagctta agacaagttt
     3841 attatatata taa
//
LOCUS       DACXMZ010000283         3838 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.284, whole genome shotgun sequence.
ACCESSION   DACXMZ010000283 DACXMZ010000000
VERSION     DACXMZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.284"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            80..682
                     /locus_tag="JD016_17905"
     CDS             80..682
                     /locus_tag="JD016_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HAU5001492.1"
                     /translation="MENYKFVFDLDSTLTKQEILPEISKCINKYELMQNLTNETMLGN
                     LSFEESFKKRVDLLKCIPISKVKSIVAKINLNEKIVKFIKENPDRCTVVTNNLDIWIC
                     DLMKELSLENKYYSSIAHSNGDFIDKIKVIIKKEDIIKKMKGPIVAVGDGSNDIKMIE
                     NADIGIGFGGVRPIAPGILKVCDYAFYEEERLCQLLKQLL"
     gene            655..1299
                     /locus_tag="JD016_17910"
     CDS             655..1299
                     /locus_tag="JD016_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HAU5001493.1"
                     /translation="MPTTKTIVVNCAGRGSRLGLNSNKSLIDIDGKPLIIRQLECLDD
                     YDDIRIVVGFQAEKLIEVVCSYRKDIMFVFNHDYLNTKTAASLSLGKKYSRDTVISLD
                     GDILFFKEDFDNFVNTDGEIIGVCQPYTKDPVYVKTDFINDGEFATEFSRTNGDYEWT
                     GLFKIYSKNLSSGTNHVYKMISPNLPLKIAHVELREIDTPEEYNEALKWYRNKK"
     gene            1312..2052
                     /locus_tag="JD016_17915"
     CDS             1312..2052
                     /locus_tag="JD016_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAU5001494.1"
                     /translation="MGNATRIYEKHVDIKKDTVQDFWNKRAKKHIPENPYISVKCNDQ
                     NPDYAKNLDKYEKEIIIPKLNITRNSNILDIGCGVGRLADDIVPICNYYLGTDFAEDL
                     ISIAKKRFSDNDCDFQVCDFINTLYDTKVKSKMPFDTILLAGVTMYINDDELKIALKN
                     LLGILDKKATIYISTPIGINKRLTLKEFYSKELNSEYNVIYRTIEEYEEILKPLLDEG
                     FEIVESKSFLTDINQYSDTERHYFILKR"
     gene            2081..3565
                     /locus_tag="JD016_17920"
     CDS             2081..3565
                     /locus_tag="JD016_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HAU5001495.1"
                     /translation="MNLMNYNKKNEVLNLIQILIEGIDYIKNNNLSESIVNSSLEAIN
                     YMKKYINNSGRSNKNLLNLIDETYVKILRLSMYKNIRLIEDCELIISNLNYLSNIIEN
                     SLNKKTKIVFMPYNAKMWNSLESIWKSAVLDEQCDCYVVPIPYYKLIDTPNGITQIYT
                     YEGNDFPEDVPVIHYDDFDLSKEKPDIIYVHNQYDDCNNATMVDSNYFSYNLKQYTNM
                     LVYVAYGILGTYPVSFYLNFYELIASRNFDKVIVQSPAFEIIAECSGINKNQILTMGS
                     PKFDSLIYNLKQKNINKNYESKLKGKIIFLWTTNLMKIPNGKDGVIDEIENVFDIIEN
                     SQEYGLIYRPHPLELEYVKSKVPECFNRYKTLLDSISIKNNIILDDSVSYYESFNLSH
                     ALITDRSSVLIEYIQTKKPILIYDIDMERGYYDSRIFDIFSNYVVGEEDMDLIKFMNL
                     VKNNNDYKLNQRLNSLNSVLSNTDGSCGEKIHTNVLEYVLNNHI"
BASE COUNT         1570 a          375 c          585 g         1308 t
ORIGIN      
        1 ttgaaatttc taactcaata ttgtttgatg tacttatcac tcaaaaatag gattttatgc
       61 ttcaatgaaa gggaatgaaa tggaaaatta taaatttgtt tttgatttag attcaacatt
      121 aactaaacaa gaaatacttc ctgagatttc taagtgtata aataaatatg aacttatgca
      181 aaatcttaca aatgaaacta tgctaggaaa tttatctttt gaggaaagtt ttaagaaaag
      241 agtcgattta ttaaaatgta ttcctatttc taaagttaaa agtatagttg ctaagattaa
      301 tcttaatgaa aaaattgtaa agtttataaa agaaaatcca gatagatgta ctgttgtgac
      361 aaataattta gatatttgga tttgtgattt aatgaaagag ctttctttag aaaataaata
      421 ctattcatct atagctcatt caaatggtga tttcattgat aaaattaaag ttataataaa
      481 aaaagaagat attataaaaa aaatgaaagg tcctatagtt gcagttggtg atggcagtaa
      541 tgatataaaa atgatagaga atgctgatat tggaattgga tttggagggg taagacctat
      601 agctcctgga atattaaaag tatgtgatta tgcattttat gaggaggaac gtttatgcca
      661 actactaaaa caattgttgt aaactgtgca ggtagaggaa gtagactagg attaaattca
      721 aataagtcgt taatagatat agatggaaag ccattaataa taagacaatt agagtgtctt
      781 gatgattatg atgacatacg tattgtagta ggctttcaag cagaaaaatt aattgaagtt
      841 gtatgttctt atcgtaaaga tataatgttt gtatttaatc atgattactt aaacactaag
      901 actgctgcaa gcttatcttt aggcaaaaaa tattctagag atactgttat atcactagat
      961 ggtgatatac tttttttcaa agaagatttc gataattttg ttaacacaga tggagaaatt
     1021 attggtgtat gtcaacctta tactaaagac cctgtctatg ttaaaacaga ctttattaat
     1081 gatggagagt ttgctacaga attttcaagg acaaatggag attatgagtg gacaggtttg
     1141 tttaaaatat attctaaaaa tttaagctct ggtacaaatc atgtatataa aatgatttca
     1201 cccaacttac cacttaaaat agctcatgtt gaattgaggg aaattgatac accggaagaa
     1261 tataatgaag ctttaaaatg gtataggaac aaaaaataaa ggagatttta tatgggtaat
     1321 gcaacgagaa tttatgaaaa acatgtagat ataaaaaaag atactgttca agatttttgg
     1381 aataaacgag ctaaaaagca tattccagag aatccctata tttcagtgaa atgtaatgac
     1441 caaaatcctg attatgctaa aaatttagat aaatatgaaa aagaaataat aattcctaaa
     1501 ctaaatataa ctagaaattc aaatatattg gatataggtt gtggggtagg taggttagct
     1561 gatgatatag ttcctatatg taactattat cttgggacag attttgctga agacttaata
     1621 tctattgcta aaaaaagatt ttctgataat gactgtgatt ttcaagtatg tgattttatc
     1681 aatactctat atgatacaaa agttaaatcc aaaatgccat ttgatacaat tctattggca
     1741 ggtgtaacaa tgtatataaa tgatgatgaa ttaaaaatag cattgaaaaa tttattagga
     1801 atattagata aaaaggcaac aatatatata tctacaccta ttggaataaa taagagatta
     1861 actttgaaag aattttactc gaaagaatta aattcagaat ataatgttat atatagaact
     1921 atagaagaat atgaagaaat tttaaaacca ttactagatg aaggatttga aattgttgaa
     1981 agcaaaagct tcttaacaga tataaatcaa tactctgata cagaaaggca ttattttata
     2041 ctaaagcgat agttttaata taaataaaat caaaattaag gtgaatctga tgaactataa
     2101 taaaaaaaat gaagttttaa atcttataca aatacttata gagggtattg attacataaa
     2161 aaataataat ttaagtgaat caatagttaa ttcatctctt gaagctatta attatatgaa
     2221 aaaatatata aataattctg gaagaagcaa taaaaacttg ttgaatttaa ttgatgaaac
     2281 ttatgtaaaa attttaagac tttccatgta taaaaatata agacttattg aagattgtga
     2341 acttatcata agtaatttaa actatttatc aaatataata gaaaatagtc taaataaaaa
     2401 aactaaaatt gtatttatgc cttataatgc taaaatgtgg aatagtttag aaagtatctg
     2461 gaaatctgcg gttttggatg agcagtgcga ctgttatgta gttcctatac cttattacaa
     2521 gttaattgat acgcctaatg gtataactca gatatataca tatgaaggta atgattttcc
     2581 tgaagatgtt ccagtaatac attatgatga ttttgattta tctaaggaga aacctgatat
     2641 aatatatgtt cataatcaat atgatgattg taacaatgct acaatggtag atagtaatta
     2701 tttttcttat aacttgaaac aatatactaa tatgcttgta tatgttgctt atggaatact
     2761 tggaacttat cctgttagtt tttacttaaa tttttatgaa ttgatagcat ctagaaattt
     2821 tgataaggtt attgtgcaat ctcctgcatt tgaaattata gcagaatgta gtggtataaa
     2881 taaaaatcaa atactaacaa tgggatctcc aaaatttgat tctttaattt ataatttaaa
     2941 acagaagaat ataaataaaa attatgaatc taaattaaag ggtaaaataa ttttcttatg
     3001 gacaactaat ctaatgaaaa tacctaatgg caaagatggt gtcattgatg agattgaaaa
     3061 tgtatttgat attattgaaa atagtcaaga gtatggtctt atatatagac cacatccatt
     3121 agaacttgag tatgtaaaat ctaaagttcc agaatgtttt aatagataca aaactttgct
     3181 agattcaata tctataaaaa ataatattat tttagacgac tctgtatctt actatgaatc
     3241 attcaactta tcccatgctt taataacaga taggtcttct gtgttaatag aatatattca
     3301 aacgaagaaa cctatattaa tttatgatat agatatggaa cgtggatatt atgattcaag
     3361 aatattcgat atattttcaa attatgtagt aggagaagaa gatatggatt taataaaatt
     3421 tatgaactta gtaaaaaata ataatgatta taagctaaat caaagattaa attctttaaa
     3481 tagtgtacta tcaaatacag atggaagttg tggtgaaaaa atacatacta atgtattaga
     3541 atacgtttta aataaccata tttagttaaa attaattaat aattagataa atgtaggctt
     3601 ggaaaggagt taaaacatta tcaattttaa taaatataag ataataaagg gcaaattaat
     3661 taaagtttga ccttttttaa atttattttg ttactctata caacatttta gactgtattc
     3721 ttatataacc agtctaaaat aaaatttatc aaaagtagag aaaattacaa attttttatc
     3781 cctaaaagtg aaaataaaag ggaaaacttt aaatagatat aaaatcctaa gcatataa
//
LOCUS       DACXMZ010000284         3833 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.285, whole genome shotgun sequence.
ACCESSION   DACXMZ010000284 DACXMZ010000000
VERSION     DACXMZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.285"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            148..1260
                     /locus_tag="JD016_17925"
     CDS             148..1260
                     /locus_tag="JD016_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAU5001496.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1468..1944)
                     /locus_tag="JD016_17930"
     CDS             complement(1468..1944)
                     /locus_tag="JD016_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001497.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2143..3768)
                     /locus_tag="JD016_17935"
     CDS             complement(2143..3768)
                     /locus_tag="JD016_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAU5001498.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALLNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         1339 a          614 c          422 g         1458 t
ORIGIN      
        1 attaaaattc taatatttga cataatatat acatttttta aataaatata tacaatgtat
       61 taaaatgtat gcaactctat ttcaaaatta ttttgtgata tatatatgtt ataataaatg
      121 tataaaattt tgattggtgg gttatatatg aatgcattta ttagaaaaag aaataaaaac
      181 tatgttgttt acctggaatt tgaagatgaa aagacaggta aaagaaaaca aaaaaatatg
      241 ggtacttttg ataaaaaaag ggaagcaagt aaaagactaa atgaggtaaa agaaagctta
      301 tataatgatg gttttttagt accaagcgaa attacagtgt cagaatttct attagatttt
      361 ctaaataaat atagtgaaaa tatatcttta gctacatata ataattatgt tagtatttgt
      421 aagaattaca ttaatccatc tattggaaaa tataaaatac aagacttaca tccggttcac
      481 atacaaaact acatagataa actatcttat aaactaaatc ctcaatcaat taagattcat
      541 ataaatatat tgaaacttgc aataaaaaga gcttatagac tcaaattaat aaaagaaaat
      601 gttatggata gtatagaggc tccaagatat aaaaaattta aaaatgagat ttatgataga
      661 gaacaaatga taaaattatt agatttagca aaaaatacag aaatggaatt acctattaat
      721 ttggctattg gattaggtct tagaatttca gaagttttag gtctaacttg ggataatatt
      781 gattttgaag aaaatacaat aacagtaaat aagataactt cccgaattaa tggttctgtt
      841 attctcaaag agccaaagac agaaagctct gttagaaaaa tatcttctcc caaagaatta
      901 atgtctttat taaaagaata taaaataaaa cagaataaaa atttattaaa atcttccatt
      961 agaaataata ataatttgtt gtttttcaat aagaaatgtg aacctattgc tgaagatgtt
     1021 atgagtaaaa aatttaaaag atttttagaa aagaatgaat tacctcatat tagattccat
     1081 gatttaagac actcacatgt tactttactt ataaattcta aagtacctat aaaggttata
     1141 tccgagagag taggacactc aaatataagt actactctaa gcgtttattc tcatgtactt
     1201 aaagaaatgg ataaagaggc ttctgacaaa atttctgaaa gtttatttaa tgctaattaa
     1261 tattaattta tcttagcttt aaattagctt agcttattga gatagattca ttaatattaa
     1321 atgtatctat ctcaaattaa taactactta aataatctgt tttattttaa tttatcacct
     1381 cagttattat acttttttac gaaaaattat actttacaat aaaaaagata ttcccttata
     1441 gaaaatatct ttttaaatac tatattatta tatacttgct aacattggaa tatctaacat
     1501 cttcttcatt acatcatcta ctttttttgc tttatattca ttgtatccaa ttaaatatcc
     1561 tttttcatat gaacttttta aaatctcttt ttcatataaa ttatcagatt tggcttctgt
     1621 gacacttaaa ttataaagcc ctttacaccc atcttcataa cctttattat accctttttc
     1681 tgcaaaatag tttttatgat ttttttcgtt atgtactgaa gatattgtta ctccagaatt
     1741 ttcttttgta ctagcataag gacaaaaacc tttattgtgt tgatgtgctg aatgtcctcc
     1801 acaataataa cgtgttaact ctgatttatt tttattatga tgacctcctg atgaattatg
     1861 ccctcctgag tgagcatatg aaaaattccc tacactaatc atagctaaac taattactac
     1921 agctactaac cttttcttaa ccataatcta ttccccccaa aatgttatat atttttatta
     1981 tatcatctac tatattatat ttaaattctt aatatacaaa atattactct attctgctaa
     2041 taaacatgtt taaacaacat aaaaaacctc ctaatatttg attaaaaatt tacacactca
     2101 tgcatagcat gtctcaaata ttaaaaggtt ttatactaat aattatttat cttttccata
     2161 cattttagct aattggtctt gtatctcttt actttctaaa tactcatcaa agtccatttt
     2221 tctatccatt attccatttg gagttatctc tataattcta tttgctatag tagatataaa
     2281 ttcatggtca tgagaagtaa ataaaagcac tcctgggaac ttttcaagtc ctttatttac
     2341 agaagttata ctttctaggt ctaagtggtt ggtagggtca tctaatacaa gtacatttgc
     2401 atttgaaagc attagcttac taagcataca tctaaccttt tctcctccag atataacttg
     2461 agcttctttt aatgcttctt cacctgagaa taacattcta cctaaaaatc ctcttataaa
     2521 actttcactt ttttcttcag agaactgtct aagccagtca accaaagaat attctactcc
     2581 gtcaaaaaac ttattatgat tttttggaag ataatcttga gaagtagtta ctccccattt
     2641 atactctcca ctgtctggtt caagttctcc cataactatg tttaaaagtg cagtagtagc
     2701 tatttcatct cccataaata ctactttatc atctctatcc aatctaaatg acacattatc
     2761 aagaacctta accccatcta tagttttagt taaattgtgt acttctaaaa cttcattccc
     2821 aatttctctt gcaggtgtaa atcctacata tggatatctt cttcttgatg gttgtatatc
     2881 ttcaacttct aatttctcta actgtttctt tcttgatgtt gcttgtttag cctttgaagc
     2941 attagaacta aatctagcta taaattcctt taattgagct atcttttctt cagtcttttt
     3001 attttggtcc ttagccaatt gtaaagccaa ttgacttgac tcataccaga aatcatagtt
     3061 tccaacgtac atttgtattt taccaaaatc tacgtcaact atattagtac atatttgatt
     3121 taggaaatgt ctatcatgtg atactattat aactatagat tcttctaaat ccattataaa
     3181 attatttaac cagtttattg atttaaaatc taagtggttt gtaggttcgt ccattaatag
     3241 tatttcaggc ttaccaaaaa gtgattgtgc taacaatacc ttaacctttt ccccaccaac
     3301 tagttccttc atttgtttgt aatgcatatc tttcgttatg ccaagaccca taagtatttt
     3361 ctcagcatta gtttctgcat cccatccgtc aagctctgca aactcacctt caagttcggc
     3421 agcttttatc ccatcttctt cattaaagtc ttccttcata tacaatgcat ctttttcttt
     3481 cattatgttc caaagtctct catgacctct tataacaaca tttaaaacag tttcttcttc
     3541 atattcaaag tggtcctgtt ttaaaactga cattctttct ttatcagtta tagatatact
     3601 acctgtatta ggctctattt ctcctgataa tatttttaaa aaagtagatt ttcccgcacc
     3661 attagctcct attattccat agcaattacc tttagtgaat tttaagttaa catctttaaa
     3721 tagttcctta tcaccaaatc taagtccaac acctgtaact tgtaacatta atatattttc
     3781 ctttcttttt gctcataatt tattttatca taaaactttg ataacatttt tat
//
LOCUS       DACXMZ010000285         3825 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.286, whole genome shotgun sequence.
ACCESSION   DACXMZ010000285 DACXMZ010000000
VERSION     DACXMZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.286"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            91..1143
                     /gene="ytvI"
                     /locus_tag="JD016_17940"
     CDS             91..1143
                     /gene="ytvI"
                     /locus_tag="JD016_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HAU5001499.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFHINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            1234..2460
                     /gene="pepT"
                     /locus_tag="JD016_17945"
     CDS             1234..2460
                     /gene="pepT"
                     /locus_tag="JD016_17945"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAU5001500.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGMDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            2667..3719
                     /locus_tag="JD016_17950"
     CDS             2667..3719
                     /locus_tag="JD016_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HAU5001501.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVIEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT         1484 a          400 c          699 g         1242 t
ORIGIN      
        1 ttatacactt ctttttattt tgtattcaaa taaaactagt atgaaatgga caaaataaaa
       61 atataaatta gaagaaagga gatttttaag atgagcatac tagaaaataa ttttttatac
      121 aaattaaaaa acaacactat ctttttttgt atatatacat tgttatttat ttttatttac
      181 aaagcgtttc catacatagc tccatttttt ctaggtgcta taatagcact tatgattaat
      241 cctatatcac aaaagttaga aaataagttt catataaata aaggtatctc tactcttgtt
      301 ctaagttttc ttgcagtggc tatagtcagt acagttacta caataatagt aataaattca
      361 atgaaagaat taatgggatt tttaaataat atatctgcta atcctgagga tataagtaat
      421 actattatgt attttttaaa taaaataaat gactttatga aatcatttca agaaatagcc
      481 aattttgatg tagaacaatt agtaaacaaa ttcagtggtg aagtaatgca aattacaaaa
      541 aatcttctta ctagtatact tggattggct acttctattc catatattat aatatttata
      601 attaccttgt ttattgctac gtactttata gcaaaagacc ttgataagat tgaaaatagt
      661 ttttataata tgtttactgt tgatgttaga aagaaggtaa aaaacgtaaa gaaagaggca
      721 gggctttcac ttgttggata tataagggcc tatacaatac ttatggctat aacatttttt
      781 gcaatctggg gaagttttgc tttatttgga ttgaaatacg gtctaatagt agggtttgta
      841 ggagctttaa tggatttaat tccattccta ggtataataa caatatatct tccagtaatt
      901 gtttattatt ttcttataaa aaattatttt atagctataa gtatgactgt gatatttttt
      961 gttttgtctt tgattagaga aatacttgaa cctaaactag tatcagtaaa tgttggacta
     1021 aatccattag ctaccctagc agcgattttt attggaatcc aagtaaaagg aataattgga
     1081 gtaatttttt gtttgggttt ggtatgtatg catgatatac ttaagaaggt ggatattttt
     1141 taattataat aattgataga tttttttaaa aataaaattt tattcacagg taaataaaaa
     1201 aatattaatc ttattaagaa agagggtata tacgtgaaac aaaaagtagt ggaaagattt
     1261 ttaaaatatg tatcatttga tacaacatct aattctcagt gtgagaactg tccatcttca
     1321 gaaggtcaaa gagttttagc taagtatata gttgaagagc taaaaacaat gggtgttgat
     1381 gatgtgagtt tagatgaaaa tagttatata atggctactt taaaaggaaa tactgatggt
     1441 atggatacta taggattcat atcacattta gatacaattg aagatgttag tggaaaagat
     1501 attaaaccaa gaataataga aaattatgac ggaaaagaca tagttttaaa tgaagcgtta
     1561 aatgttatta catatgtaaa ggattctcca gagttggaag aatttaaagg tgacgattta
     1621 atagtcactg atggaacaac tctacttggc tctgatgata aagctggtat agctgagata
     1681 gtgactgcaa tagagtatct aataaatcat ccagaaatta agcatggaga tataaaaata
     1741 ggatttacac cagatgaaga aattggtaga ggtgctgatt tatttgatgt tgaaaaattt
     1801 ggagcaaaat atgcatatac tttagatgga ggaatagttg gagaacttga atgtgaaaat
     1861 tttaatgctg caaatgcaac cattactata catggtagaa atgttcatcc aggttcagct
     1921 aaaaataaaa tggtaaatgc tatacatata gctgctgaaa tatcagaaat gtttccagca
     1981 gatgaaagac cagaaacaac agaaggatat gaaggatttt ggcatttaaa ttctataggt
     2041 ggaaatgtcg agaatgtaag tatggcttat attattagag accattgtag agaaaaattt
     2101 gaaaatagaa aatctattat gatagagaat atagaaaaaa taaataagaa atacgataat
     2161 agagtggaat tggatttaaa agactcttat tacaatatga aagaaaagat tgagccagtt
     2221 atgtttatag ttgatatagc taaagaagct atggaggaat taggtataaa accacgttta
     2281 gttcctgtaa gaggtggtac tgatggagca agattatctt ttaatggatt accttgtcca
     2341 aatatattta ctggtggatt aaacttccat gggaaaaatg agtgtatacc agttagttct
     2401 atggagaaag ctactaaact aattgtaaga atagctgaaa aatatgctga aagagtttag
     2461 ttaaaaagta tttaaaaatt aagcatttaa agttataata gctatatcat tctttataag
     2521 tcaagctgat tataagcttg tgttgtactt aaaatggtat agctattttt gtaattattt
     2581 ttataaaaga taattatttt tagtttaggg tatcaataaa aagtatgaat atcaataggt
     2641 attaacattt aaaaggggga attgtattga aaattaaatc taaaaaaata atagcatttg
     2701 cactttcact tgggattatg ttgccaagtt gtgtatatgc agacactatt agtaaaaatc
     2761 aaaatgcaca ggtagatatg aacaattatc ttcaaggtag aaaatctcca aaggaggaaa
     2821 aacttattgg tgaagataga tttgatacag cgataaaaat atctcaaagt ggttggaata
     2881 atggttcaga aagggtgttt ctagttaact caaattcatt gccagatgca ttggcttcaa
     2941 ctcctttggc aagtaaattg gatgcaccaa tactgcttac aaataaaaat agtataccat
     3001 ataatgtaat agaggaaata aaaaggctta atccagcaga agttatttta attggaagtg
     3061 aaggagctat ttcatcaagt gtaaaagaat ctcttgaaga tatggaagta ttagtcagta
     3121 gaatcggtgg agcagataga gaagaaacat ctttacttct tactagacag ctagatgata
     3181 ctggtgattt aggtgtctca aaagtggcag tggttaatgg gtataatgga ctggcagatg
     3241 caacttcaat atcaagccca gctgcaagtg ataatacagc tataatatat acaggtaagg
     3301 atagcataag aagtgaggca aagaatttta ttacacaaaa ctcaactaag acatacatag
     3361 taggtgggga gtacagtata agtaaaaaac ttgaaggaca gttagtaaac tcagaaagac
     3421 tagcagggac agatagaaaa gatactaatg cgaaagtttt agaaaagttt tatggaaaat
     3481 caagtaaggt taataatatg tattttgcaa aagatggaag tggtagagaa gctgatttag
     3541 ttgatgggct tgctgctgga gtattagcat caaaaacaaa gtcacctgta gtacttgcaa
     3601 gtggaagttt aagtagtgca cagaaaagtt ttataaaaaa agtaaaggct gataaatttg
     3661 tacaagttgg tggaggtaaa aatagtaaac catatgcaga agcattagcc ttacagtaat
     3721 attgtgtaaa tcaaaattaa tatataataa tagcaagtaa accaagttgc acttcaatat
     3781 atataataaa ataagtaaac taacctatgt ttaaatataa tatat
//
LOCUS       DACXMZ010000286         3788 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.287, whole genome shotgun sequence.
ACCESSION   DACXMZ010000286 DACXMZ010000000
VERSION     DACXMZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.287"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     repeat_region   154..1891
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=352..380
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            2193..2318
                     /locus_tag="JD016_17955"
     CDS             2193..2318
                     /locus_tag="JD016_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAU5001502.1"
                     /translation="MKIISERLGHTNIKITINRYSHLLNDMDKKASENIIKVLFK"
     gene            2538..>3788
                     /locus_tag="JD016_17960"
     CDS             2538..>3788
                     /locus_tag="JD016_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAU5001503.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKL"
BASE COUNT         1515 a          518 c          483 g         1272 t
ORIGIN      
        1 tttttataat tattctactt ttatatttta atcattaaaa ctaaagtcaa tattatgtgg
       61 taattattta ctacaaatga taaaaaataa ttatatttta aaggagaaaa tgctgtataa
      121 attattgggg tattttacct atacttgttg caaatttaca taccacatag ttaatataaa
      181 acaaatttca aagagattgg acaacgaata aaaattgcaa tttatatacc atatagttaa
      241 tataaagcat atttgtgtcg atatatagtt ctaactaagt ataagattta catacctcat
      301 agttaatata aaacttggag ttgtaaaagc tgtttctatg ttcagtcgag tatttacata
      361 ccacttagtt aatataaaac atcctttagt ataatatttt caagtaaaat ttctaaattt
      421 acataccact tagttaatat aaaaccatac aactgacaaa agcactataa atacatactg
      481 agatttacat accacttagt taatataaaa ctattgttga ccttgtaaaa tcttcaaaga
      541 agtccatgat ttacatacca cttagttaat ataaaacact tataaattga gtaatcgggg
      601 tggtgattag tttatttaca taccacttag ttaatataaa actagaggtt aagtgtgcaa
      661 attatgagtt gaaaaaataa tttacatacc acttagttaa tataaaacta taatttgttc
      721 aactaaccca attggcgaag ataacattta cataccactt agttaatata aaacacccat
      781 gaaagctata ataaaaaaat tgctggatga atttacatac cacttagtta atataaaact
      841 gtagagataa gcaggagtgg tctaacagta aatcaattta cataccactt agttaatata
      901 aaacaaagga gctgttccca attatagcta ttttggagct tatttacata ccacttagtt
      961 aatataaaac gtgaaccttg tatttgcaac attaacatct aaaatataat ttacatacca
     1021 cttagttaat ataaaacatt aaaaatacta ataaagcaga tataaaacct agcgaattta
     1081 cataccactt agttaatata aaacaaatca cgaccgtacc tttagtaaaa tagcaattat
     1141 catttacata ccacttagtt aatataaaac tcaaataata gctcaataga cctagttata
     1201 aaagacattt acataccact tagttaatat aaaacccaaa tatttagaga tggtgtagag
     1261 tgctaaaaat ttatttacat accacttagt taatataaaa ctatgtttca aagctaaaag
     1321 tagacatcac ctctttattt acataccact tagttaatat aaaacaatgt tataccacca
     1381 aacttggcac tattatttgc acatttacat accacttagt taatataaaa cgatgttgaa
     1441 gttgacggag ttaaaaaaag tattttaaat ttacatacca cttagttaat ataaaactat
     1501 cgaaaacacg actagcccac acactaaata ctgctaattt acataccact tagttaatat
     1561 aaaacgaagc aagtaaaaga ttgaaaatag ataaagtatt atttacatac cacttagtta
     1621 atataaaaca cgagtgcatt aggtataaca gttggatttg taataattta cataccactt
     1681 agttaatata aaacttcgca gtctgtaatt gttgttggta gagctaaatc gatttacata
     1741 ccacttagtt aatataaaac ccgcatggca agaaacgact gtacaggatg ttagaaaatt
     1801 tacataccac ttagttaata taaaacagaa gatggattgg atgtttatgt tcctaataat
     1861 tcatttacat accacttagt taatataaaa ccccaaaata aactaagtat tttcagtact
     1921 tacacacata caactctctc aaatttgcag tgaaccatga gtagtgcaat tgataacatt
     1981 tatcacacac cctcaatccc ttacattgca actgttaaac cctactttat caaaaatatc
     2041 gctcactgca aaatttatat atttttatta tatcataaaa ttcaaactat aaaattaaaa
     2101 tggaataatt tatcaattta agaacaccta catatttgta aatacttact atattatcta
     2161 ttctaatagt tcttcttcta gctagaacta atataaaaat catatctgaa agattaggac
     2221 atactaatat aaaaataact ataaatagat attctcatct tttaaacgat atggataaaa
     2281 aagcttctga aaatataatc aaagttctct ttaaataaca ttccatttta tgtaagtagg
     2341 cattatattt ttcttaaatg tcacttaaat gtcagttata tataataata gtaaacaact
     2401 gtatttcact aatatttgct attatctaat agtatttata gtttagattt gatataatac
     2461 atgtggtaaa attttgattt gtaaaacaaa attttaccta catatattaa aacaaattta
     2521 aaactaagga gacctatatg ttaaaagtat taaaaggaaa tataattttt acaaaaactt
     2581 cagaaacttt cacagtattt gaaaatagtt atattatact ggaaaaaggt aaagtaaagg
     2641 gaatatttaa agagatacct aaggaataca aaaacctaaa agtagtagat tacactgata
     2701 agcttataat acctggtatg aatgacctcc atgcccatgc ctctcaattt aaaaatcttg
     2761 gaatggctat ggataaagag cttttgccat ggcttgaaac ttatacattt ccagaagaat
     2821 caaattataa agatatagaa tatgcaacaa aaatgtataa aaaatttgta aaagaacttt
     2881 ggaaatctgg aactacaaga atagcagtgt ttgcaacagt acataaagaa gcctctatga
     2941 aacttatgga tatattcaaa gaagttggtc ttggagcata cgttggtaaa gtaaatatga
     3001 atatgaattg ccctgatact ttattagaaa acactcagga ttctattact gatacagaag
     3061 aaattataaa taagtatagt gatacagatt ctattgtaaa accaataata acacctagat
     3121 ttataccaag ctgtacaagt gaacttttaa aaggtttagg agatttatgt ttaaaatata
     3181 atattcctat tcaatcacac ttatctgaaa attatgatga gattgaatgg gttagaagtc
     3241 tagaacctga atctaaattc tatggagatg catataatag atataattta tttggtcaaa
     3301 ctcctacttt aatggctcat tgtgttcatt gttctcagga tgaaatagat ttaatgagag
     3361 aaaataatgt tatggcagtt cactgtccag cttctaactt taatgttggt agtggtgcta
     3421 tgcctatcag aaaattaatc gataataata ttagattagc acttggatca gatataagtg
     3481 gtggacatac attgtcaata tttaaagcaa tggtatctgc aatacaatta tctaaattat
     3541 attgggttaa ctctggtaag aagtataatt tcttatcttt atctgaggct ttttatattg
     3601 caaccaaaag tggtggaagt ttctttggaa aagttggtag ctttgaagaa ggttatgatt
     3661 ttgatgcatt gattattgat gattcaaatt taaatcatga caattattct atacttgaaa
     3721 gacttgaaag atttatatat gttggtgatg atagaaatat cattcataga tatgtatgtg
     3781 gaaaacta
//
LOCUS       DACXMZ010000287         3732 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.288, whole genome shotgun sequence.
ACCESSION   DACXMZ010000287 DACXMZ010000000
VERSION     DACXMZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.288"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            43..711
                     /locus_tag="JD016_17965"
     CDS             43..711
                     /locus_tag="JD016_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001504.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            870..1376
                     /locus_tag="JD016_17970"
     CDS             870..1376
                     /locus_tag="JD016_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HAU5001505.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGNRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1504..2520
                     /locus_tag="JD016_17975"
     CDS             1504..2520
                     /locus_tag="JD016_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HAU5001506.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2874..3578
                     /locus_tag="JD016_17980"
     CDS             2874..3578
                     /locus_tag="JD016_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HAU5001507.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1607 a          318 c          650 g         1157 t
ORIGIN      
        1 atattaggta ttttaaataa attttttaaa gggtgatttt ttatggaaaa tataactagt
       61 tattttacaa caatactatg tgtatttata tgcttatcat ctgtatttat atttactcaa
      121 ctagctagag tatttattaa taagaagaag attaatcaaa aaattaaatc aagaaacgga
      181 tttaggtatg atagagattt tattgaagcc agaagagaag aaatacatat aaaggataac
      241 aataataata aaaataagag taacaataag aaattaaagg aagaaaaagt atttaaatac
      301 gataatggtg atttatataa aggtgagttt gttgatggaa agaaaaatgg atttggaata
      361 tatatttttt caagtaagga aaagtatgaa ggattatgga aagatgacaa gatgcatgga
      421 ataggaaaat atacttatag agatggttca atatatacag gtgaatttaa atacggatta
      481 aaaaatggtt tgggaaaatt aacatatccc aataatgaca tttataaggg gtatttttta
      541 gataataaga aaaatggtaa gggtgtatta tataaaaatg atggaaataa acaagcaggg
      601 atatgggaag atgatgaaca atgtaaaagt ttagatttta aagatttaaa taataataaa
      661 ataaataatg tttatcaaaa tagattaaat aacaataaat taaaagttta attattctaa
      721 taaaaaagtc tttatttatg atggaatata ggatatatct atgttgtaat ttgtaaaatc
      781 ttcctatatc atataaaatt aagtattgat tagtctatta cataaatata gaatataaat
      841 atatccattt aataataagg agaagagata tgaaagagta tgtagttgtg gatttagaaa
      901 ctacaggatt agatccatat aaaggttgtg aaataataga aataggaata acagaaataa
      961 aaaatgaaca aattgtaaaa aactattcaa ggttgataaa acctaagggg ataatttctt
     1021 catttataac agaattgaca catataagta atgaaatggt agaaaatgaa gaaccactag
     1081 aattagtttt acctagattt agaaaataca taggtaatag aactataatt gcacacaatg
     1141 cgaaatttga tttaaaattt ttaaattact atcttagaat gttagattta gaaccaataa
     1201 acaattatat atgtacagta gagttattaa aaaaatgtaa aagttataaa ggaaaaaata
     1261 agaaacttga aacagcttgt gcttattaca acatagagaa tataaatgct catagagctg
     1321 acagtgatac attagcaact gcaaaactat ttttgaaaat aaaagatgaa tattaattat
     1381 aaatatagag aaaattagtg aaaatatcct ttataaaatt gacccttaaa cttacaattt
     1441 tgttctttga tttattttgt agaacaatgt ataatgtacc ttatatgaag aagggagact
     1501 aaaatgggtt attacaatga taatgataat aataaaataa gacgtaaaaa aattagtaac
     1561 aacaaagata gtaataaaaa atctaaacat tcttatactt cggcaaataa aaaaaataaa
     1621 agtcctaata caagttccag taaaagaatg agtgaaaaag tagattcaca gaatcttata
     1681 aaaagagata gagatagttt tacaagagat aatgtaagac ctttaaatac agattacaat
     1741 caaagagaaa gaagaagagt cgaatataaa caaagagaga aaaaattaaa aagtaaaaaa
     1801 agaaaaaaac aaaaaaggat tagaatagtt agggcattat tatctattat gttagttgca
     1861 ctaattatat tttcagtggc aggtgcaata tttacagttt cagctataaa agggtcacca
     1921 gaagttacta aaaagttaat taggtctaaa tatataagta gtgaggttgt aagtataaaa
     1981 caaatgccaa atgatttaaa acatgcaata gtttccatag aagatgagag attttataag
     2041 cataaaggag tagatgttat ttctctagtt aggtcagtat taaacaatgt gtttacagat
     2101 acaacacaag gtgcaagtac tatagatatg caagtttcaa aaaacctgct tactagtaat
     2161 gataaaacta tgaagcggaa aataagggat atgtataatg ctaagcagat gaataagatt
     2221 atgactaagg atgaaattct tgaagcatat ttaaataata tgtatctagg taaaaatgca
     2281 tatggagctg caaagggagc tgaagtctac ttcggtaaaa aagttagtga attaaattta
     2341 gctgagtgtg ctatgttagc tggtatgaca aataatcctg caaggtatat tgaccatgga
     2401 gaggctaaga agagacaagt gattgtactg tataagatgc atcagcttgg ttatataact
     2461 gataaggagt atagaatagc aaaagctgat gacactccat ttaaatcaga aattgattaa
     2521 aattgtaaga atatagtatt ttcaatagtt taaacaaaca aaaatgtaat aaaaaaggtg
     2581 atttgccacc catttgccac cataattata atctaatggc aaattgtttt aatgtacaaa
     2641 ctagataata gaatattgtc ttaaatgcta tattatgcta aaatttaatt gtattttgta
     2701 aattaaaaaa cttataattt agttttctag ggttccgcat tttataatgg tcaggtccaa
     2761 gagaaaacgc acaattgttg tgtacacgga aggataaaag cctgggagat atttaataat
     2821 atttctatag gcttttttat ttttacatat tttaaatttg aggaggtttt ctaatgaaaa
     2881 aatttgttat tgaggatgca ttttggaatc tatttcctag tgcaaaaatt ggagttgtta
     2941 tttgtaatga tatagataac tctataaaag atgaagacta taagaaaata attttgaagg
     3001 gagaagaaga agcattaaaa tacttggagg actcagaatt tagcaacaac caagtgatta
     3061 aagtatggag agaagcattt aaaaaattta aaacgaaaaa aggagcaagg tcttctattg
     3121 aagcattatt aaaaagagtt cataatggaa ataatatagg aactattaac cctttagtgg
     3181 atatctacaa ttttatctca ttaaaatatg cattaccttg tggaggggag gatatagata
     3241 aatttatagg agatattagg ctaactagag caattggtaa tgaggagttt atcccactag
     3301 ggacagatga aaattcattt ccatatgaag gagaaataat ttataaagat gatggtggag
     3361 caatttgtag atgttggaat tggcgtgaag cagtgagaac aatgcttact gaagatacta
     3421 acaatgcctt tttatgcatt gaattagtag atgaaagtag atttaaagaa tttgaaaatg
     3481 ctttaaatga attagctaaa atagttcaag aaaaattggg tgggaattgt aaaatttcaa
     3541 ttttagatat tgataataaa gaggtatcag tggattaata tggaggtgct gttataatgg
     3601 actattctag ttataaagat acttatattt agggtaagaa ggtctatttt agatataacg
     3661 attatcaaag gatataatat agaaaataaa aatgtagaat ataaaaagct tttaaaataa
     3721 gaaaaaaatg ta
//
LOCUS       DACXMZ010000288         3731 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.289, whole genome shotgun sequence.
ACCESSION   DACXMZ010000288 DACXMZ010000000
VERSION     DACXMZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.289"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            365..1054
                     /locus_tag="JD016_17985"
     CDS             365..1054
                     /locus_tag="JD016_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5001508.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            1054..1971
                     /locus_tag="JD016_17990"
     CDS             1054..1971
                     /locus_tag="JD016_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAU5001509.1"
                     /translation="MELFLVVISIVLFAFYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHIFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            2044..2970
                     /locus_tag="JD016_17995"
     CDS             2044..2970
                     /locus_tag="JD016_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001510.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            2967..3647
                     /locus_tag="JD016_18000"
     CDS             2967..3647
                     /locus_tag="JD016_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAU5001511.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSILGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITILMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIIFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
BASE COUNT         1427 a          391 c          614 g         1299 t
ORIGIN      
        1 agtttgtttt taattattta aaatccaaat ttgattattt ttaaagaaac tatgttctgt
       61 ataaaactag aatgtggttt cttttttatg tataaaatat tttttagcaa gagttaaaag
      121 gtgttttata atataaaata gttaattgta gatgttaata aaagaaaatt atgtatattt
      181 cagataaaga taagagagta tactatattt taattatttt ttcaataaag cttattgaaa
      241 agaactttaa cttagaagag tatcaccaaa ttgcagatac aatatcataa acagttattt
      301 attgacaacc ctttttaata aatatatcat ttgaagtata ctgacgaaaa ggagttgaat
      361 aataatggac atcttattgg ttgaagatga taagaatatt cgagaaatgg tatacgattt
      421 tctgattagt gagaattact ctgttaaaac tgccattgat ggaaatacag ctattgaaat
      481 atttaaaaat tattcatttg atattatttt actggacttg atgttaccaa agtctagtgg
      541 ttttgaagta ttaaaatata tacgaagtgt gagtgttgtt cctgttatta ttactactgc
      601 gaaagatagt gacacagata aaatgctagg gcttaatctt ggtgcagatg attatattac
      661 aaagcctttt tcaatcgtag aattgcttgc aagaattaaa gctaatatta gacgtgcaac
      721 agtgtacagc agttcaagaa actctgaaga tatggttatt cagtataaag atttgattat
      781 aaacatatca aaatatgtag tttcaaagaa tggaattgac ttaaacttaa cacatacaga
      841 atttgaaatt gttaaattac tagcgactaa tcaaggacgt gctttttcaa aggaacaatt
      901 atacaacact atttggaaag aaccttatta tggaaatgaa aatgtgctaa atactcatat
      961 taacaggtta agaaataaat tgtctgatgg tatagaaaaa aataattata ttaaaactct
     1021 ttggggaatt ggctacaaaa tggaggaaga ataatggagt tatttttagt tgtaatttcg
     1081 attgtactat ttgctttcta cttaaaagaa cgaaataagt caaagaaaat ggaaagtgat
     1141 tataagtata tcaattcaag acttcgtgac ataacaaatg atgatgaaat taattatatt
     1201 ttagtaccat ctgatatttc gattgtaaaa gaaacagcag tagatataaa taaacttctt
     1261 gaaaagtttt acagcaggca aattaattat aatagaagtg aaaaagcgat attgcaaata
     1321 tttacaaaca tatcacacga tttacgaact cccatcacag tattaaaggg ttatattgaa
     1381 atgctatatc ttcaaagtca aaaagaagaa ttatcttctt caatgagagc aactgtggaa
     1441 aaaatgcaaa caaattcaaa tgagcttgtt aattcaataa ataacctgtt taatatggca
     1501 aaaatccaat ctggagatat gatattaaat cttcaaaaat ccaatctaac acaattatgt
     1561 catgagatta ttttagagtt ttatgatttg ctagataaag aaaactttaa tgttgaagtt
     1621 aatattgaag atagacctat atatgcaaat gttgacacag aagcattaca acgtatactc
     1681 aaaaatttaa ttgataatgc tataaaatat ggttcagatg gtagattttt agggatttca
     1741 ctatatgaaa aggaaaaaca tatttttata gatattgagg ataaaggttc aggtatttct
     1801 gaaactgaaa aagagcatat ttttacaaga gcttatactg ctgacagaaa aaaaggcaat
     1861 ggtttaggtt tggctatttc tcaagggctt gtaaactcta tgggaggttc aatttatgta
     1921 aatagtagac ctaatgtaaa aacagttttt acggtaaaat tgaaaagtta gaaaaaagtt
     1981 agatttaaaa gagagaaaag ataattttat tttgtatact gtttttagaa aggagggagt
     2041 atgatgaact atatcataga gacccatcaa ttaaagaaaa tatataaaga taaagctgtt
     2101 gttaatgctg ttaatattca tgtgaaaaag ggagaaattt atggatttgt tggaccaaat
     2161 ggagcaggta aaagtacagt aatgaaaatg ttgctaaatc ttgttaagcc aaattctgga
     2221 gaaatagtta tgttcagtaa gaaagtggca gaaactgatt ttgaaatatt aaaaaagatt
     2281 ggtactatta ttgaaaatcc gtatttatat gagaatttga ctgctaaaca gaatcttgat
     2341 ttacactgtg aatatatggg ttattataac aaagaacata tatcagaggt attggaatgt
     2401 gtcggattgt caaagcaatc aaacaagaaa gtttcaaaat attctttagg gatgaaacaa
     2461 cgtcttgcaa tagctagagc aattcttact aaaccagaat tattgattct tgatgaacct
     2521 ataaatgcac tcgacccaga ggggataaga gaaatgcgag aattattccg taagttaaat
     2581 acagattatg gaataactat atttatttct agtcatattc tttcagaggt agaacaaatt
     2641 gcggacacta ttggaattat tcaagatggt aaacttatta aagaaatttc tatgtcagat
     2701 atacacaaat atcaaacaga ttatgttgaa gtagatgtag ataatgtgga acttgctgga
     2761 tacttgttag aaaaagaatt tggaataaaa gactttaaaa ttacttcgga aagttgtatt
     2821 gaaatatatg atttacgaaa agatgtaaaa gaaatatcca aaatatttat tcaaaatcaa
     2881 attggtataa atagcattgg acgtaaacaa agttctttag aagattactt cttccaaact
     2941 acaggtacag gaggtaaaga aacactatga tacatttaat gaaactagag ctaaaaaaag
     3001 ttggactaaa aagatatttt ttattttcaa tactggggat tttcttatgt atgttttttg
     3061 tctttactgg attgaatgat aaatctactt caatatatga ttatgatgtt acatttagga
     3121 caatcagttt atttttctgt ttttactata ttatattatt tgctgtgttg aatgtggcat
     3181 atataataaa tgaatataca aataaaacaa tcttgatttt attctcttat ccacttgacc
     3241 gtaaaaaact tattttatca aagttacttt tgattacgat tcttatgata tcatcaatga
     3301 tagttggata tatctgttgt tttacattcg ttgttgtgtt ggataagtat tttaatttag
     3361 ttattggaga ttttcatatt tcaattttat cttattggat accaggagca attaaaagta
     3421 ttattatatt ttgctcatta ggtatatgga cattcgttgt tggaatgata agaaaatcaa
     3481 tacctgttac ccttgtcagt tcaattattt ttatcaattt aagacaattt attttagctg
     3541 gtacaaatac aactcaagac agtttgattt ctgtttttat tatagttggt ataactgtga
     3601 ttggtatata ttatatacta aatcataaag ttactgaaat tgactagata ctgtggctag
     3661 gaaggtagtt attaattgta tcttcttggg ataataatga tttgctatta tatcagctat
     3721 tattaatatt a
//
LOCUS       DACXMZ010000289         3681 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.290, whole genome shotgun sequence.
ACCESSION   DACXMZ010000289 DACXMZ010000000
VERSION     DACXMZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.290"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            784..1536
                     /locus_tag="JD016_18005"
     CDS             784..1536
                     /locus_tag="JD016_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001512.1"
                     /translation="MLKVNQLYKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            1526..3637
                     /locus_tag="JD016_18010"
     CDS             1526..3637
                     /locus_tag="JD016_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAU5001513.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVINKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDNYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMNEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANKDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLSNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         1379 a          533 c          480 g         1289 t
ORIGIN      
        1 actacagact tttattatta caatctctta tttattacag ttctttgcta tactacagac
       61 ctttattatt acaacatttc tattatatta caatctctta tttattacag ttctttatta
      121 tactacagac ttttattatt acaacatttt attattttaa cttctttact ataatatagt
      181 aatattttaa tatctcaaaa tcatatcaat cgcttttcag catagtctaa aatacttcca
      241 ctttctaaaa ttatctatca tacctatact aacaatactt tttaaccttt ttattataca
      301 tttcaattgt ttttaacaac ctatttattt ccaaaactcc aatatatttt ctcaattaca
      361 ttgtgttaat aacccctaaa tcctttcaaa ttatttacaa tacattccat taaataatct
      421 tactgctcta taattctttt tttcttttta accctactat acattattta catcattttt
      481 ttcaatacaa cattcgttat tctataacct ttttactttt actatatacc ttttttctaa
      541 ctacaccttc aatgttccac atcatattac tttagcacaa caattaatac ctaaaaataa
      601 ccctctaaat cctccaataa tataatcaat ttaactataa taataacctc tcacactatc
      661 caaacaatat attttcaaac attacaaaaa aacatccaac tattacaata tagctcatat
      721 ccctcctccc ctttcaaatg ctatcattga cttacaagat aaatgaaagt gaggatgata
      781 tatatgctta aagtaaacca attatataaa tcatataaaa caggtagtaa aacatatcct
      841 gtattaaaaa atatttcttt tgaaatcaac aaaggagagt ttgtcgcaat catgggttca
      901 tcaggaagtg gaaaaactac tcttttaaat tgtatttcat gttttatccc aattgataat
      961 ggggaaataa ttcttggaaa taataatata agaaatttaa aagaaaagga actttcagaa
     1021 gttagaaacc aacaactagg atttgtattc caagatttca tgctatttga tggtcttagt
     1081 gtatttgaaa atatatgtgt tccacaaatt atcaaaggta gtcaagtctt acaaatggaa
     1141 aaccgtgcaa ttaatctatg tgagctattt ggtattgaac atatcagaaa aaaataccct
     1201 gccgaaatat ctggtggaga aaaacagcgt acagcagtag caagagcact catgaacaac
     1261 ccagatttaa tattagcaga tgaaccaact ggtaaccttg atagtaagtc ttgtcgtaca
     1321 gtcattgaat catttttaaa agcaaagcaa gaattagatg ctactatact catggttaca
     1381 cacgatagct ttgcagcaag cttctgtgac cgagttattg tgcttaaaga tggaataatt
     1441 tataaagaag ttgtaaaatc aaattcaaga cataaattcc tagatgaatt actagaagtt
     1501 ttaaaagaaa tgggaggcgc tcaagatgac aattaataaa ttatacagta aacttcgagc
     1561 ctataattgg aagaactata caactttatt cttttgtata atactttctg tggtattagt
     1621 aacttcatat gctctaattt attttagccc aacaatacaa aacatacttc caactggtgg
     1681 agatagcaga aaacaggcta caatgatatt ctcagttgct atattaggat gcgctgtctt
     1741 tactacatac gcttcttccc tctttttaaa atttaaaagc aaggaaattg gtattttcat
     1801 ggctctagga acagataaga agaaattaaa aaaacttcta ttttcagaga tattgttaat
     1861 ttcaatagta tgctgttttt taggtcttat attaagtatt cctgtatctt ttggtatatg
     1921 gaaaatattt caattagtga ttattgatac cagagaaatg acatatcatt ttggaactca
     1981 aggattcata tatggattta tatttagtgt atttgtgaca ttatgcatat ttatactttc
     2041 tgtaaaattt attaatcgta caaatataat tgatatacta aatgaccaga gaatatctga
     2101 accaatacag gatgtaaagg actggtatgg tatattaggt ataattttta ttatactcgg
     2161 tatttttcta ggatatctcg taccttcact aactataaac aaattaaatt atagactacc
     2221 atctgtatgg agctttactt atctactctc tcttattgga atatatatga ttatgacata
     2281 catagttgta catgctaaaa ggggaaaaca tccagagaaa tactataaaa acatcatatc
     2341 aactagcatg atgagattta tgggaagaca aacagtaaaa aacatgtgtg ttatttcatt
     2401 tttgattgca ggagcattat tcgctgtatt ctatgttcct aatatggtta caggtatcta
     2461 tgatactctt aaaaataacc ctatagatta tacttttacc tacaatcaaa gagataaaca
     2521 ggtatcaaaa aaagatattg aacaattagc agataaacac aatataaaaa ttcaaaacta
     2581 tatggaaatt ccttctatag aattaattgt tgatggtgta gaatcaatat atcataaaga
     2641 tggaactatc gaaaaggtat atatgaaaaa aagctcctat gatatgttct ttaattctaa
     2701 agatttttcc aagctaattg gcaaaccaat aactataaaa aatggtgaat atcttgctgt
     2761 tataaataaa ggaaatgaaa atcaagatta taaatatacc aaatttatat caagcccagt
     2821 cacagaaaaa gaaatcccct ttagatatgc tggaaaaata atatttaatg gttctttttt
     2881 tcaaaataga agtatgaacg cctatgtcct taatgatgag gattataaat catttactaa
     2941 agatttatcc ataaaaaatt acaataattc tatactattt aatgtagata aagataacta
     3001 tgcttttgcc aatgacttaa agaaagaaat tataaaaaga acatctaagg aaatgggcat
     3061 gttcagtggt tatgatgaat acgtaaaaaa acgttatgaa tcaactggaa gaaaatattt
     3121 tatgaatgaa gaatatccaa gtggtgaagg tcatttaaaa ctagacccaa acaacaatca
     3181 gttgtatctt aattggaaat actatcctaa ctttaaaatt ttacaaagcc aagatatgat
     3241 aaaaaacatg gcagtcttct taatgttatt catatatgtg gcaatcatat gttttacagc
     3301 agtaggaatc attgcatata caaggggtgt tactatagct ataaatcaca agcaggtctt
     3361 tatggattta aatcgattag gagcaaacaa ggattatata aagttctgta ttagaaatca
     3421 attaaagaaa gtattttcat accctcttat tgcaggaagt tttttaattt atctgtttaa
     3481 ttttatgatt atgttaagta atgatggtag aatcgtcaat agtgaggtca atgctctttt
     3541 gataaactta ggttttatag ggatttgtgg ggtttatatg taccttatat atctattaac
     3601 ttacaagaaa ttcaaaaaaa ttattggtat tttataataa ggaaggttgt tagattttta
     3661 aagtaataaa atcattggtg t
//
LOCUS       DACXMZ010000290         3670 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.291, whole genome shotgun sequence.
ACCESSION   DACXMZ010000290 DACXMZ010000000
VERSION     DACXMZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.291"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(80..445)
                     /locus_tag="JD016_18015"
     CDS             complement(80..445)
                     /locus_tag="JD016_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAU5001514.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            complement(503..1009)
                     /locus_tag="JD016_18020"
     CDS             complement(503..1009)
                     /locus_tag="JD016_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAU5001515.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     misc_feature    complement(1052..1180)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1232..1930)
                     /locus_tag="JD016_18025"
     CDS             complement(1232..1930)
                     /locus_tag="JD016_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAU5001516.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     gene            complement(2001..2426)
                     /gene="rplK"
                     /locus_tag="JD016_18030"
     CDS             complement(2001..2426)
                     /gene="rplK"
                     /locus_tag="JD016_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAU5001517.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            complement(2465..3007)
                     /gene="nusG"
                     /locus_tag="JD016_18035"
     CDS             complement(2465..3007)
                     /gene="nusG"
                     /locus_tag="JD016_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HAU5001518.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            complement(3027..3248)
                     /gene="secE"
                     /locus_tag="JD016_18040"
     CDS             complement(3027..3248)
                     /gene="secE"
                     /locus_tag="JD016_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAU5001519.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            complement(3274..3423)
                     /gene="rpmG"
                     /locus_tag="JD016_18045"
     CDS             complement(3274..3423)
                     /gene="rpmG"
                     /locus_tag="JD016_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAU5001520.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
BASE COUNT         1006 a          738 c          448 g         1478 t
ORIGIN      
        1 ttctttataa ttccaaatac tttctttata gttttttata aataaaaaaa gatacccaat
       61 gggtatcttt ttaaagaaac tacttaactt ctactttagc tccagcagct tctaatttct
      121 cttttatttg atcagcttct tctttagaag ctccttcttt aactgtctta ggagcattgt
      181 caactacttc tttagcttct tttaatccta atcctgttat ttctctaact gcttttataa
      241 ctccaacttt tgaagaacca acgtcagtta atactacatc aaactcagtt ttttcttcag
      301 cagctggtcc accagcagct gcaccagcta ccattactgg agctgaagct gatacaccga
      361 atttttcttc agcagcttta actaattcat ttaattctaa aactttcata ttttctatag
      421 cttctaatat ttgttctatt gtcattttta gcacctccga attttatttc aaattaatat
      481 ttataatttt ttagttaagc aattaagctt cttcttgacc ttctgccttt ttcgctattg
      541 catctattaa gtatgcgaag tttgatactg gagcttttaa gctaccaagt aatttagcga
      601 taagaacttc tcttgatggt atgttagcca tttcaacaat cttagattca tcatagaatg
      661 caccttccac aatacccatc tttaatttca tttttggatg agaatccatg aatcctttta
      721 atattctagc tggagcaact ggatcatcat atccaaatgc tatggcatta gttccaacta
      781 ataattcatc attaaattgc tctataccaa cttctttagc tgctcttcta actaaagtat
      841 ttttgtatac tttgtaatct acaccagctt ctctcatttg ttttcttaac tcagttactt
      901 cttcaactgt taatcctttg taatctacaa caacagcagc agaagatttt tgtaactttt
      961 caactatctc agaaacaact tctgatttaa tttctatagc ttttctcata tagatgtgca
     1021 cctccttcac caaataggct tagtatttac cgcaacatta taaaaagctt ttgttgccaa
     1081 aagtagacat caaaagcttt agtaatctca atttatctaa aacctcggcg ggatattaag
     1141 ctctcgcacc cactatctac ggcaaatcta ttctttttta cattgacaag tgatataata
     1201 acatttttgt caacctgtgt caacagtttt attattcagc tgttcttgct gggtttattt
     1261 taactccagg tcccattgaa gaagctactg ttatacttct taaatattgt cctttagcag
     1321 cagctggttt agcttttatt atagcatcca ttaatgcagt aaagttttca gttaattttt
     1381 ctccaccaaa tgatactttt cctactggaa cgtgtattat gttagttttg tctaatctgt
     1441 attcaacttt accagctttt atttcatcta tagctttagc tacatcaaat gtaactgttc
     1501 ctgatttagg gtttggcatt aaacctttag gtcctaatac tctacctaat ctacctacta
     1561 cacccatcat atctggagta gcaactacta tatcaaagtc aaaccagttt tcaccttgta
     1621 ttttttgaac taattcttca gctcctacaa agtcagcacc agcttgttca gcttctttag
     1681 ctttttcacc tttagcaaaa actaaaactc tttttgtttt accagttcca tgaggtaata
     1741 caactgcacc tcttacttgt tggtcagcat gtcttgagtc aacacctaat ttaatgtgtg
     1801 cttctactgt ctcatcaaat ttagctccag ctatatctga aactaatgtt aatgcttctg
     1861 atgcatcata aaatttagtt ctatctactt tttgtaatgc acctgcatat cttttacctt
     1921 ttttagccat tttattttcc tcctcgtggt taatttcggc ttctagcctc ccactttata
     1981 aaaaaaagtg tatcttttaa ttagtcttcg attacaacac ccatgcttct agcagtacca
     2041 gctatcatac tcatagcagc ttctactgat gcagcattta agtcaggcat ctttaattca
     2101 gctatttctc ttacttgagc tgaagtcatt ttagcaactt ttttcttgtt tggttctcca
     2161 gaacctgatt ttaagtttaa tgctttttta attaaaactg cagctggtgg agtttttgtt
     2221 ataaaactga atgatctatc ttgatataca gttataacaa ctggtattat cattccagct
     2281 tgatctgcag ttttagcatt aaattctttt gtgaatccca ttatattaac accatgttgt
     2341 cctaatgctg gtccaactgg tggcgctgga gtagcctttc ctgcaggtat ttgtaatttt
     2401 atttgaccta taactttttt agccattttg agcgcacctc ctcagatttt tctataatat
     2461 agtattagat tttttctata ccttcaagct ctattacaaa tagagtcctt ttaccaaatg
     2521 catttatgca tactttgact tctctatttt ctaagtcaat ttcttctatg ttacctattt
     2581 gtccactaaa tggtccttga gatactttta ctgtatctcc tatttcaaaa tcaatatcgc
     2641 tatttactac tttagggtct gttgtgtcta tccccatagc tttgacttcg tcttcactta
     2701 atggcactgg ttttgaacca ggtccaacga aacctgtaac tcccttagta tttcttacta
     2761 cataccaaga ttcatcagtt attatcattt taaccaaaac ataacttgga taaactttac
     2821 gttgtctagt tttttctttt ccagttttag tagtttccac tacttcttct gttggaacaa
     2881 ctacttgtct tatgcagtct tccatacctc ttgttttaac tgctttctca attgtagctt
     2941 taactttatt ttcatgtccc gaataggtat gaactacata ccaacttgct tcttgtaatt
     3001 ctgacattta acttacctct tttcgtctat tttagtaata acgctagtgc accagataat
     3061 atagtgtcta taccccatac taatatagtg catgaaataa ctacagttaa aactatactt
     3121 gtgtttttaa acagttcctt ttttgtcggc catgtaactc tttttaattc ttgtttagtt
     3181 tcttttaagt atccaaacaa actaaacctt tttttagttt ttgcaccgtc atttttttgg
     3241 gcagccattc catactcaca tccttataaa aaactatttt gtttctttat gagttgtatg
     3301 ctttttacag aatctacaat atttttgtaa ttctattctg tctggattgt ttttcttatt
     3361 cttagtagtg ttgtagtttc tttgcttaca ctctgtacat gctaaagtta ctttaactct
     3421 catctgatac acctccaact ttgtaataca ataatatata ttgttaatat atgtgaatat
     3481 aatatcttta aaatatgtat ttattaaaaa tctacagtat tcaagatatt attaacttcc
     3541 tataatcatt accttgtata agttatcaca aaatcctatg ctgtgtcaat gtttttgtat
     3601 ttctatgtta aaaaaagagg agcataaccc ctctttttgt tattcctttt aatccatcag
     3661 tttagtaaag
//
LOCUS       DACXMZ010000291         3624 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.292, whole genome shotgun sequence.
ACCESSION   DACXMZ010000291 DACXMZ010000000
VERSION     DACXMZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.292"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            170..1546
                     /locus_tag="JD016_18050"
     CDS             170..1546
                     /locus_tag="JD016_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAU5001521.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1857..2528
                     /locus_tag="JD016_18055"
     CDS             1857..2528
                     /locus_tag="JD016_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAU5001522.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2717..3514
                     /locus_tag="JD016_18060"
     CDS             2717..3514
                     /locus_tag="JD016_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAU5001523.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1372 a          402 c          671 g         1179 t
ORIGIN      
        1 tatatataaa aatttaaaat attccaaaca gaaattataa aaattactat cattgtcttt
       61 atacaatatt tatactataa tagaataata atttagagga aagctctaaa aataaacaaa
      121 ttaagggtct taacagtagt gattttatgt taaggaggta taaagaaagt tgaaaacaat
      181 agttagaaga cttattctct acgcagatgg aatgcgtcct actcaaataa tggtatctgg
      241 gtttgctgcg attattgtaa tcggagcatt gttgttaaca ctcccgatag cgtcgcagag
      301 tggagagagt ataggcttat taaatgctct atttacggct acttcagcgg tttgtgttac
      361 tggtctagtt atggtagata cagcaactta ttggagttta tttggacaga tagttataat
      421 aacattgata caaattggtg gtcttggatt tatgaccgta gccacaatgt tttcacttat
      481 ggcacgaaaa aaaatacaac taagagaaag attattaata caagagtcat taaatcaagc
      541 tgatttatcg ggacttgtaa gacttacaag atttgtatta ataataacca taacaataga
      601 gggaataggt gcactggttt tatcaactgt ctttattcca caatttggtt tgtctaaagg
      661 gatatggtat agtgtatttc acgcaatatc agcgttttgt aatgccggat ttgacttgat
      721 gggcagtgta agtggcccat ttacatcgtt aaattcttat gtaaacaact ttacagtatc
      781 aatgactgtt tgcgcactta tagtgcttgg agggctagga ttccctgttg tacttgatat
      841 tgtaagaaaa agaagatttt caaaattaaa tgtgcattct aaggttgtat tgttttcaac
      901 tgctacactt atttttgtgg gtgcattatt tatttttctt atagaattta atcaaaaagc
      961 tactatggcg gacttgcctt taaagggaaa agtactatca gcaatatttc aatcagtaac
     1021 agcaagaact gctggtttta atacgcttga tttagctact ttacgagaaa gtagtgtatt
     1081 tgtaatgata attttgatgt ttattggagc atcacctgca tcaactggtg ggggtataaa
     1141 aacaacaaca ctagctgttt taatcattac agttagaagt tttctatcag gaaaatcaga
     1201 tatagaagct tttgaaagaa gattagctcc atcaactata aaaaaatctc ttggtatatt
     1261 tgtaattagt atatcagcag ttatttttgg aacattgatt atttcaataa ctcagcctaa
     1321 ttttacatta gttcaatcag cttttgaggt tacatcagca cttgctacag ttggttcaag
     1381 tttagctgga actccaaact taaatgcttt aggtaagatt ataataataa tttttatgtt
     1441 tatgggtaga gttggttctc ttactttatt tatggctata ctttctggtg gtagaagaaa
     1501 gagtcaacca atcagatatg cagaaggtaa aattatggtt ggttaagtta ccaaggagta
     1561 gtataaattt ataatggatt tagagtagca ctgtaaatct gttgataaaa taaatgagcc
     1621 tagatgtaat tatatgaaga aataaaatca actaacgaac aaaattaatt aatgaaataa
     1681 aaaaacaggg aaataaaata caaataaaaa ggtaaatcaa aattaaatta atctgtgtat
     1741 ataaaattat atattgtgta taagttttgt atgtgtaaag tatataagtt ttgtatgtgt
     1801 aaacaattaa tttaagagat attatataaa ttgtgtatta gggaggaagt ttcgagatga
     1861 agcaatatat agtcattggg tgtgggagat ttggaagttc agttgcgtct actatgcatc
     1921 ttttaggaca tcaagtaatg gcaatagaca aaaatgaaga ttcagttcaa agtatatctg
     1981 acaaggtaac ccattcactt atagtggatg ttactgatga gcaagcgtta aggtcattag
     2041 gtttaggtaa ctttgatgta gcagtagttg caataggttc tgatataagg gcatctataa
     2101 tggcgactct tatagccaaa gaaatgggtg tagagttgat aatatgtaag gcaaaggatg
     2161 aattacaagc taaagttctt tataaaattg gtgcagatag agttgtattt ccagaaagag
     2221 atatgggagt aagagttgca cacaatttag tttcggataa tatattagac catattgaac
     2281 ttgacccaga gtattcaatt gttgaaatcg taactccaaa tagttgggtt ggcaagacac
     2341 ttatagagct tgaattaaga gctagatatg agataactgt acttgctata aaaacaggta
     2401 aaaatataaa tgttacacct tctccagatg aggaacttac agccggaagt atcctagtta
     2461 taatcggtca aaatactagt ataacagcga taacatctgg aaataagggg ataattagaa
     2521 gaagataatt tactatttaa tatatattta attgcaatga aagtagatag tatcatataa
     2581 ttatgaatag ttatatgata ctattttttt aatcgaaggt agtatttatt tgtaagatta
     2641 gataaagaga agttaaatta aaagtaagga ggctgtgcta atataaaaat ttataagtta
     2701 ttagcattta gataaaatga ttacaaatat aaacagtaag gataatgaaa agttaaagta
     2761 tacaagagca ctattaaaat caaagaatag gaataaagag tcaaagttca taatagaggg
     2821 atacagaata gtaatgcttg cacttgaatg tatggcaaac cttgattatg tatttatcaa
     2881 tgaagaattt gaaaataaga aagaacatgt aaaactatta gaagatttgg ataaaaaaaa
     2941 cacaaagata tacaagacta ctaataaaaa ctttaaagaa ttagtggata cagaaaatac
     3001 tcaaggaata ataggtgtag tttcatttaa gaaaaaaaaa ttaagtgaaa gtataaataa
     3061 aaaagataaa tttgtattga ttttagacag aatacaagac ccaggaaata tggggactat
     3121 aataaggact gctgattctg ctggagtaga tgccataata gcactaaagg gatgtgtcga
     3181 tatatacaac ccaaaagtaa ttaggtctac tatgggttct atttttgata tgaatataat
     3241 tgatgcttca caagatgaaa ctgtggacat gcttaaatca ttggatttta atatagtttc
     3301 aagttactta aatacagaaa atttttatga caaaatagat tatggctcaa aagtagcatt
     3361 ggtgatagga aacgaagcaa atggaataaa tgaagaactt gtatcaaagt ctgatatttt
     3421 ggttaagata cctatatatg gtaaagccga gtcgttaaat gctgcgataa gctctgctat
     3481 actgatgtat gaaataaaaa aatacttaat ttaatgtatt gtaataaata taatgtcatg
     3541 ttataatctt gaattaaata gtagagtatc aaaaatagtc aatatatatt aaaaaaataa
     3601 ttaaattaat tatatattag atgt
//
LOCUS       DACXMZ010000292         3593 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.293, whole genome shotgun sequence.
ACCESSION   DACXMZ010000292 DACXMZ010000000
VERSION     DACXMZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.293"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            90..1586
                     /locus_tag="JD016_18065"
     CDS             90..1586
                     /locus_tag="JD016_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAU5001524.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            1720..2646
                     /locus_tag="JD016_18070"
     CDS             1720..2646
                     /locus_tag="JD016_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAU5001525.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNDVSQNGIQRSTKSSIKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            2764..2955
                     /locus_tag="JD016_18075"
     CDS             2764..2955
                     /locus_tag="JD016_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001526.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            3178..3528
                     /locus_tag="JD016_18080"
     CDS             3178..3528
                     /locus_tag="JD016_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001527.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
BASE COUNT         1443 a          345 c          676 g         1129 t
ORIGIN      
        1 tataataatt tatagaaata aataaaaaat acaatataca taatagtaga aatatagcaa
       61 aaaggtgtga tttataggga ggattactta tgtttgaatt tgaaacgcaa ctaaaaaggt
      121 taaaacatga agttttaaca gcagtagcaa gactatctat tgaagaaaat ttaacaaaag
      181 aaaatataga aaaaattcca tatgaaataa ttaaaggaga aaaggcgaga tatagatgtt
      241 gtgtttataa agagagagaa gtagttcttg aaagggcgca acttgcatta agtttaactc
      301 caaatagtaa atatggagat ataaatccag attcagttag tttggacaca gatgagcaaa
      361 ttatatatat agttgaagca gcatgtgata gatgtccaat aaatgaattt acagttacag
      421 agctttgtag agggtgtttg gctcatagat gtaaggaagc atgtaaattt ggggcaattt
      481 cttatataaa tgggagagca tatataaatc atgatttatg taaggcttgt ggaatgtgca
      541 aaagttcatg tcaatatgat gctatatcag aagttgtaag accttgtaaa agcgtttgtc
      601 caacaggggc tttggatttt aatagaaaca ctatgaaagc aatgatacat gaagataatt
      661 gtataaattg tggagcatgt atgtcagcat gtccttttgg agcaatatct gataagagtt
      721 tgatagcacc tgttgcaaaa aaacttgcaa agaaagaaaa tatgtatgca atagtagctc
      781 cagctataac aggtcaaata gatagtaata ttacatatgg tcagttaaaa aatgctataa
      841 aatcactggg atttgtagat atgatagaag cttcttgtgg agcagatgca gttacagttc
      901 atgaaagcaa tgaacttatt gaaagaataa aatcaggaga taattacatg acaaattcat
      961 gttgtccggg attttttaag tatatagaga agatgttccc agatgaaaaa gatagaatat
     1021 catctacagt gtctcctatg gtagcaacag gaagattagt aaaaaaaata gattctgatg
     1081 ctaaagttgt atttataggt ccatgtacag ctaaaaagag tgaggcaaca gagaaaagtt
     1141 taaaagattc agtagattat gtacttactt ttgaagaact aatggcatta tttgaagcat
     1201 ttaatataga tttatcaaaa tgttcaaatg aagatataaa tgatggttcg atttttggaa
     1261 ggggatttgg agcatctggt ggacttgcca aatcagtaca gaactatatt gctgaaaagg
     1321 ggcttagttt tgattttgaa cctataaaag tatctggtcc aagacaaatt aaaaagacaa
     1381 tgacaatggc taaattggga agattagaag gtaattttat agaaggaatg atgtgtgaag
     1441 aaggttgtat taatggagca ggtaagtttt tatcaggtgt aaaagctaaa aatacttttg
     1501 atagaaaaaa tggacaatct actaaaaaga gtgttttatc taatgataag attaaagaat
     1561 ttgaagatgt aaatttagaa agataattgt tttggtacag tcattttttt attatgccac
     1621 aaatatgcct aaaatttgcc ctttttatat gtgtatttta cagagttaag gttgttatac
     1681 tataaatatg taataaataa aatttatagg agcttttgga tgaggaaaat agtattaaaa
     1741 gatatcctgt ttttagttac tacattatct ataatagtaa tattattgct tgtaacaaat
     1801 ataactacta atagtaagat aaaatcaaca agtgaatctt atttaaaaag tgacaaaaat
     1861 gatgtgagtc aaaatggtat ccagaggagc acaaagagta gcataaaaaa tactgcattt
     1921 aagactacta tactaaacac tggaaagtct gattgcatat tggttgagat aggtaacaaa
     1981 gtcataatga ttgatacagg agaagataaa aatggaaaac aaatagtgga taggttaaaa
     2041 gaaaaaggaa taaatacact tgactatttg attttaactc atttagataa ggaccatata
     2101 ggtggagtag atagtgtgtt atctagtgtt aaagtaaaga atattataca agcaaattat
     2161 aagaaagata gtaagcaata tgatgaatat attgactcac ttaaaaaagc tgatatagaa
     2221 ccagtgctac taaaagataa tatgaatatt gtaatcaata gtgcagaaat aaacatacat
     2281 ccggcttcaa aaagcaagta cgagagttca aatgattatt caataatcac taatataagc
     2341 tatggagcac ataagttttt atttgcagga gatgcagagg aagaaagatt gtcagagttt
     2401 ataaatggaa atactttaaa atatgatttt gttaaaatgc ctcatcatgg aagatatgac
     2461 aaattaactg aaacattttt agagtctatt tcaccccaat atgctgttat tacatgttca
     2521 gagaaaaaag aaccagagga aggtgtttta aaaatattgg aaagattgaa tataaaaaca
     2581 tttttgactt ctaatggaga ggtagttatc aatagtgatg gtaagacatt gagtgtaaat
     2641 caataatttt tttaaaaata tctaaaaaaa tttaaaaaaa tcattagcaa atattacccc
     2701 ttaaacatag tatattttag gagctgaaac tccacataca aaatgactag gaggtaacat
     2761 attatgttgg atagattttt tggtgaaggc ggatttggtg gatgtgcttg gtggataata
     2821 atattattct tcttattctt agcattccaa gattgttggg ctgaaatgga cttatgtgca
     2881 tggatacctt tcttaatatt attattaata ctttgtgaat gtggtgacgt ttttgatgga
     2941 ggatgtggat gctaaaaaaa tcaatttaga tgatttaata aagcttatgg tgggtataaa
     3001 taaaatacca aaccaatcac aatctagtgt ttgaaggtag tcggaaatcc cccgctacct
     3061 tctttttttt attgttaaaa tttatttttg aattatttca tttacatata ataattttta
     3121 cgataaagtc tgatataatt aaaaagaaaa taaaatttat ttataggaga tgtaaaaatg
     3181 gctatagtgg ataagatttt caatgagata aaacttatgg ttgcaagact tttattggga
     3241 aaaaaataca gtgaatatga agaaaaagat ttagtgtata atacagaaga agaagttata
     3301 cttataactt taaaaagatt agtttttcag ggaaatgtaa acgaagcaga agaaatttta
     3361 tttgataaag ctaagagtgt aaatagtgaa aatatgcagt atatagcaat agaattttat
     3421 actatgctta tggaaaagac tgatgaagaa ttagaagcta tgaatttttc taaacaagaa
     3481 gtttatcaag gtatagaaga tattagaaaa gtattgaatt tagaataaaa gatatatgaa
     3541 aatattgaaa taaattaatg ctaaggtgag aatattatac ttatatataa aaa
//
LOCUS       DACXMZ010000293         3587 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.294, whole genome shotgun sequence.
ACCESSION   DACXMZ010000293 DACXMZ010000000
VERSION     DACXMZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.294"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            98..1678
                     /locus_tag="JD016_18085"
     CDS             98..1678
                     /locus_tag="JD016_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAU5001528.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            1852..2298
                     /locus_tag="JD016_18090"
     CDS             1852..2298
                     /locus_tag="JD016_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAU5001529.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2319..3362
                     /locus_tag="JD016_18095"
     CDS             2319..3362
                     /locus_tag="JD016_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAU5001530.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1450 a          335 c          686 g         1116 t
ORIGIN      
        1 gtgattttat ttatgatatt tatgggatta aaaagatttc aatagatatt tttggtaagt
       61 atttaagaag tctttaactt tataggagtg ataatttatg ctaataggta catctaaatt
      121 aagcagaaat gttgatactg catgtgtaga agaagtaggg attccaatga ttgtattgat
      181 ggaaaatgcg gttatatctg caatgaaaaa tatggatata gacatgtata atagttatac
      241 agttgtatgt ggtgttggaa ataatggagg agatggtctt ggaatagcaa gacatctaaa
      301 tacatatgga aaagaagtaa acgtattttt agtaggtaat cttgaaaaac taagtgaatg
      361 ctctaaaata aattacaatg tacttttaaa tatgggaatt aatattataa atattaataa
      421 aaatgattta agtgaaaaaa attatggaga tgaaaatgaa gtagataaaa ttattaaagt
      481 tgagaaacaa aatattataa aaaataaaaa tttaacaatt tttaaagata gagtatctaa
      541 aagtgatgtt gtagtagatg ctatatttgg gactggatta aaacgtgata taactggtat
      601 ttttaaagaa gttattgaca tagtaaacga taatagcaaa aatacatatt ctatagatat
      661 tccttcaggt atagatagcg ataatggaaa tgtattagga acttgtatta gagcaaacaa
      721 aacgattagc tttgagtttt ataaaagagg ttttctaaac tatgacacta gcacacttat
      781 aggtgatgta gttgtagaaa agataggtgt tccagatttt attacaagaa aataccatga
      841 tgaagagttc ataactgata agacttttgt aaaaaataat attagaagaa gagataagca
      901 tggttttaaa agtgattttg gaaaagtatc tatagttgca ggttcaaaag gattttatgg
      961 tgcaagtttc atagcaactg aatctgctgt taagagtggg agtggacttg taacattaat
     1021 tagtagtgaa gatgtgcaag aaaaggtatc tattagactt acagaagcaa tgactttaaa
     1081 ttttgaagaa gaaagattaa ataaattact taattctagt gatgcaattg gttttggtcc
     1141 aggtatggga gataatagtc aaacatttga taaattactt aagatagtag aaaattctaa
     1201 ttgcccaata gtattagatg cagatggatt gaacgttatg aaggacagat gttataagtt
     1261 tttagaatgg aaaaatagat ttgtaattac acctcatttg ggagaaatgg ccaggttgac
     1321 aggagattca attgggtata taagagaaca tagagtagat gttgcaaaag aatttgctca
     1381 aaaatataat ctggtggtgc ttttaaaagg ttatcaaact gtcataacag atggtaaaaa
     1441 aacatatata aatcctacag gaaatagctg tatggcaact ggtggaatgg gtgattgttt
     1501 gcttggaatg attacttcat ttataggtca aggtatggat atattggaag ctacagttag
     1561 tggagcatat atacatggct atataggaga taaattgagc aaagcaatgt atacggtaaa
     1621 tgctacggat ttaataagta acatttcatt aacaatgaaa gagttgttgg atatataatt
     1681 ggtgatattt gatactactt tgtaacaatt ttaacaaagt atgttgatat aatactatta
     1741 attaattaat ttattttatt gttttgtaat ttaaatttat attcaaaaac attgtataaa
     1801 catataattt atatagggtt ttataattca tatgaaaaga gggtagatag tatgaagtta
     1861 atatggttga tagtagcagt cttatttgga atagcagaaa tgttaacacc aagcttaaca
     1921 ttgatatggt ttagtgtagg agctgtaata cttatatttt taagtagctt tatagagagt
     1981 attttcttac aaattttaat atttgcagta atttctatag caatgttagt cgtagctact
     2041 aaaaaaatag taaaaaaaga taaaggctat aaatctaaca ctaatttaca agcaatgatg
     2101 tctaaaaaag gtattgttac agaagagatt tctccaaata acacaggtct agttgtagtt
     2161 gaacatgaaa catggacggc tatttctata gatggtgaaa agatagaaaa aggttctaca
     2221 gtagaagtat taaaaattga aggggtaaaa ttagttgtta aaaaagtaga cgccacagtt
     2281 aatgtgacaa accaatagaa taaaggagga tttatagtat gggtactaaa attgttttaa
     2341 gtattgtatt aatagtagta gtcgtagcaa taagtttaac ttgtataaga gttataaaac
     2401 aatctaaagt aggtataata atgagacttg gtaagtttca aaaagtggct gaaacaggtg
     2461 tacatttctt aataccattt ttagataaaa tggcatatgt aattgacctt agagaaattg
     2521 taatagattt tccacctcaa ccagttataa ctaaagataa tgtaactatg cagattgata
     2581 cagttgtata ttataaagtt acagacccag ttagatatgt gtttgagata gctaatccaa
     2641 tagctgctat tgaaaattta acagctacaa cactaagaaa tataattggt gaacttgatt
     2701 tagatgaaac attaacatca agagatataa taaatgtaaa aatgagaact atccttgatg
     2761 aagcaacaga taaatggggt ataaaagtaa atagagtaga gttaaaaaat ataatgcctc
     2821 ctcaggatat tcaagttgca atggaaaaac aaatgagagc agaaagagaa agaagagagg
     2881 caatacttca agcagaaggt aataagtcag ctgcaatact tcaagcagaa ggtgaaaaac
     2941 aatctgcaat attaacagca gaagccaaaa aagaagctat ggtacgtgta gcagaaggtg
     3001 aaaaagaatc tgctatatta gtggcagaag gtgaagctga agctataaga cagacagcta
     3061 ttgctaaggc acaaggtgaa gctgaaatga taaaaagaac tcaaatggca acagcagaag
     3121 gtttaaaatt agttttttca gcaatgaaag aggctgatat agacaacaat attctagcat
     3181 taaaatctat ggaagctctt gaaaaaatgg ctgaaggtaa gtcaacaaaa ctggttttac
     3241 cttcagaagc agttaatttc ctaggaacat tcaaaggaat aaaggaagtt atgagtgatg
     3301 ataacaaaga agtacttgat ataaaagaag ttttaaatga taatgaatca ttaaaaaaat
     3361 aaaagagttt tgttatcata ggaaggatat ggaaattaaa ggtcgaatta attaaatata
     3421 gcacaaaaat gcacgttgaa aagaagagga cttataaatt tttaagtctt cttctttgtt
     3481 tttattagaa agatgaaatt ataaaaataa attaacagaa tctttataca atttatcctt
     3541 tactaatgta aaaagtgtta tataatatta tataaaacat atagcaa
//
LOCUS       DACXMZ010000294         3556 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.295, whole genome shotgun sequence.
ACCESSION   DACXMZ010000294 DACXMZ010000000
VERSION     DACXMZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.295"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            8..661
                     /locus_tag="JD016_18100"
     CDS             8..661
                     /locus_tag="JD016_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAU5001531.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            790..2073
                     /gene="brnQ"
                     /locus_tag="JD016_18105"
     CDS             790..2073
                     /gene="brnQ"
                     /locus_tag="JD016_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAU5001532.1"
                     /translation="MSKQMRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLI
                     GFVVADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCAT
                     TYEMGIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVL
                     IVKGIISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSD
                     KDQIGMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQV
                     GKVILGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSI
                     IAISVPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVP
                     FIQSLPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2275..3462
                     /locus_tag="JD016_18110"
     CDS             2275..3462
                     /locus_tag="JD016_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HAU5001533.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
BASE COUNT         1296 a          396 c          659 g         1205 t
ORIGIN      
        1 ggggaaaatg actaaaatta ttaaagtgca aaaacctaaa tttacaggag aattagaaat
       61 tattgaaaat cttgaagata tacttgaaga tatatttaat gatgaaaaaa tatttaataa
      121 aattatagaa aatgaagtta taagtgattt agattctgta agagttagct ttgaatcttg
      181 tatatttaga aatgtgtctt ttgaaaattg taatttaaaa aagattgatt tacttgatgt
      241 ggtatttgaa aactgcaatt tatcaaatac attttttgat ggaggaagta tatacagagt
      301 tgaatttaaa aatagtaaat tgataggagt tagatttgat gattgtatac ttaaaaacgt
      361 tttgttcaag gatgttttag gtaagtattc taacttttca ttctctaaat ttaagatagt
      421 ttctatctta gattcaaatt ttgaaaattc tgtatttcaa gaagtaaaag gtgacaatct
      481 agtatttgag tatacaaatt taaaaaaagc aaactttaat ggaactaaac tttctggcat
      541 agattttact actaataata ttgagggaat tgagattggt atagatgata ttagaggagc
      601 aatttttgat gtgtcacaag caattgattt aacgaaattg atgggaatta tcataaaatg
      661 aaaaaaatat tcataaaata aattgggaaa acgttaacaa tgtgtggatt tttaccagat
      721 tgacattttt ctgaaaataa agtaagatta tcttaatatt caaaatattt tcaaaggggg
      781 taatttagtg tgagtaagca gatgagagat atttttatag ttggttttgc tttattttct
      841 atgttctttg gtgcaggaaa tcttatgttt cctccatttt tagggatgga atccggcaaa
      901 gactggttaa ttcctcttat tggatttgtt gttgcagatg ctggtatagc aatgcttgtt
      961 attatagcaa cagcaagatg taagggaagc atggatgatg tacttaggag agctggtaaa
     1021 gggttagcaa gagtaatgag tgtagctgct ctagcatgtt tagcacttct tgtaatacca
     1081 agaacttgcg caacaacata tgaaatgggt attatgccta tatttggaga ccatggtcca
     1141 gtagctaaag ctgtattttc aataatcttc tttggtctaa ctttagcatt tactattagg
     1201 tcttctaagg ttatagacat aataggtaag tatttaactc cagcgttatt attagtatta
     1261 tttgttttaa tagtaaaggg tataatttct ccagcaggac ctatgagtcc tgagcatatg
     1321 atagataaaa atctatttgg tgaaggtata tcacaaggtt atcaaacaat ggacgctcta
     1381 ggtgcagcat ctatggcaac tataatgttg atgtctataa tagctaaagg atatacatca
     1441 gataaagacc aaataggaat gactattaaa gctggttttg tagcctgtgt atttctagca
     1501 gtagtatatg gtggtcttac ttatttaggt gcaacagttt caactttata tgatactagt
     1561 atacctcaag catctttatt agttgaaata actagtatat tgttatatgg tcaagtagga
     1621 aaggtaattt taggtgtaat tgtggcactt gcctgtctga ctacttctac aggtttaaca
     1681 gcatcaatat caacttattt tgaaggaata agtaaggcga gttataaaca actggttata
     1741 ggtatatgtg tagctagtat gcttatatca aacttaggtg ttgattctat aatagccata
     1801 tcagttccaa tcctacaaac tatatatcct gttttattag caatagttgt tatggagttg
     1861 gctggtaaac atataaagaa tgataatgct tttaaaggtg cagcatatgt tacattagtg
     1921 attagtttat tatcagctat aaatggaatg acaggagctg ttccatttat acaaagcttg
     1981 ccacttgcag gtttgggctt taactgggta ataccagcta tattgggtgg aatagttggt
     2041 aactttataa aaagcaacaa acaagtagca taataatttg taattaattt aattttataa
     2101 tatattaatt tttagtacta tgggtaggtt taatctaaac ctaatctacc catagtactt
     2161 tttagattgg attataatag catgtatata attttttata atataatttt tctttgaagt
     2221 atgataaaat tagtaatagt gataagaatg tacaaattta ggaggtttta attaatgctt
     2281 agaaaaataa cagcatttat gctatccata tgtttaatta ttggacttac aggttgtagt
     2341 ttacttgtag gcaagaaaga taaagagaaa attatagatg attttaaagt tgcagtggta
     2401 actcaaccat tatctgaaaa taaagtacaa tacaatatgg tggaagagat ggcaaaagaa
     2461 tatgaggaag agaataaaat tgataaagac aaagatggtc aaactaaagt taaacaaact
     2521 ataaagcatg ttgtattgcc agagaatttt acttctaata tagacagtgc tattaataag
     2581 atagtaaagt tggctgacga taaagaagtt caggctattg tagtttcaac tgaccaagca
     2641 gggcttttac ctgctttaca aaaggtaaag gaaaagcgtc cagaaataat aactatttct
     2701 gcccctatgg gagatgataa aaaccaatta agccaatttg ttgatgtgaa tctaggggtt
     2761 agtgcagagg aaagaggaaa agtattagct gaacgttcaa aagaaatggg agcaaaagct
     2821 tttattcatt atgcctctac agatgatttg aaagatgtta atatagccaa aagattagaa
     2881 atgattaaag agacttgcaa aaatatagga ttgccatttg tacaagttaa tactccaaat
     2941 ataaatactg aagaagacaa aaacaaagtt aagcagtttt taaatgaaga tattgaaaaa
     3001 caagttaaaa aatatggaaa agatataaat gtgtttggtg taaatgagta tatggatgaa
     3061 gttatattga ctaaggcttt agaattaaag tatatagttg cagagcaaag caatccatca
     3121 ccaatacaaa catatcctag tgttatgggt ctaaaaatat ctgaaaaaga tgctcaaaat
     3181 tatgataaaa taaacgacat gataagtgaa aaggctaagg cttttggcat gtcaaataga
     3241 cttggtggat atcctatgcc aatggatgct tttctaccat cagtagcaat ttatcttgct
     3301 actgaaatgg tgaaacaaga tttaacacaa gaagatgtat gtgacccaga ttatttagag
     3361 gcgtttacag aattaagatt tggaatcggt tcagaattta ctcctttaac agaggtactt
     3421 tataattatc aatcagtaat tttaagccaa ttaatttatt aatattaagg acgtgtctca
     3481 aaatgaactt tttagtttat gaggcactct ttttctgtta aatcaaatat atttttatct
     3541 ttttaacaat taaaaa
//
LOCUS       DACXMZ010000295         3544 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.296, whole genome shotgun sequence.
ACCESSION   DACXMZ010000295 DACXMZ010000000
VERSION     DACXMZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.296"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(192..1208)
                     /locus_tag="JD016_18115"
     CDS             complement(192..1208)
                     /locus_tag="JD016_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAU5001534.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            complement(1391..1753)
                     /gene="spoVAE"
                     /locus_tag="JD016_18120"
     CDS             complement(1391..1753)
                     /gene="spoVAE"
                     /locus_tag="JD016_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HAU5001535.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            complement(1767..2780)
                     /gene="spoVAD"
                     /locus_tag="JD016_18125"
     CDS             complement(1767..2780)
                     /gene="spoVAD"
                     /locus_tag="JD016_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HAU5001536.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVCTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            complement(2947..3384)
                     /gene="spoVAC"
                     /locus_tag="JD016_18130"
     CDS             complement(2947..3384)
                     /gene="spoVAC"
                     /locus_tag="JD016_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HAU5001537.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
BASE COUNT         1148 a          639 c          380 g         1377 t
ORIGIN      
        1 ttgttaaaat aacggatttt caaataattg aataaaatta ttcattcata ttataaaaac
       61 tatctcaata aataaaggag tacaatgtac ttatatacac ctactccttt taatttttat
      121 ttatattatt tgttaaaaaa cttcacataa tctataatat ttttaataaa cgtttgatac
      181 ttaaaagctt actactttat tcttttaacg ctatttcgta ttattaacct tggatataat
      241 tttcttggaa tgtgttctgt actcttttta tttattatat ttactaaact atctactcca
      301 ttttttataa cttgctctct tgggtcatct attgttgata aatgcgacct aaaatatgat
      361 attaattcaa tattattata ccctattatg gaaatatctt ctggtacatt tataccataa
      421 gtagttttta actcttgtat tatagaaatc gctggtacat cttgttgtac aaatatagct
      481 gaagcttttt ttattttatc aatgtatttt tctaaaaatt caacttgaga ttcaaaatcc
      541 attttagatt taatgacttc tgtctttaaa tttgcttctt ccatagcatt caaatatccc
      601 ttggttctat cttcatgcat ttttgaactc ttatcatctg aagttactgt tattatatct
      661 ttatgaccat ggtcaattaa atgcttggta gctatataac ctccatacac gttatcgtcc
      721 caaaagaaat tatctacttc tcctataaaa gatggtttaa aatatataaa tacatgtgga
      781 aaattattgt cttttaaaaa atcatactct tcttttttta ttgattgaga cacaattact
      841 aatccgtcaa ccatttgtcc atttaccatt ttatatacat tactatcacc actaatgtta
      901 ttattaggct gtattataaa atcgtattta ttagtctcaa tagaatttat agaactatcc
      961 attatatcac taaaaaactt tctagtatct tgatgattaa aattatttga aaagattacc
     1021 cctatcctgt tagtttcttt ttttgctaaa tttcttgcat ttacattaaa atggaaccct
     1081 agtctatttg cctcttctac cactttcttt tttgtttttt cagatacatt aggattattg
     1141 tttaagcacc tagaaacagt tgaataactg atacctagat tccttgcaat atctttaata
     1201 gttaccatct ttatccccta cttcacttca ttttattttg ataatataat actacactat
     1261 gttgtaaata attgtcaact tcaattatgt aaacgcttct ttatataaaa aataggtgga
     1321 atctaaaaga tatccacctg ctttataaaa agttattatt tcaaaaactt attattcttt
     1381 taataaagat ttatggtttt gcttttggag taaatactac tgacataata tatccaaaga
     1441 atatagctgc tgctattcca ccagctgtag cagttgtacc tcctaaaaaa atacctaaaa
     1501 aaccatcact ttgaattgat tttattactc ctgtagcaag tgaatatcca aatcctatta
     1561 taggaactgt tgcacctgct gctgcaaact taactaatgg ctcataaagt cctagaaaag
     1621 ttaaaactac accagttgtt acatacgtta ccataacata tggagtttgc attttgaaat
     1681 aatccatcat tacttgggct attaaacata taattccacc tacaataaat actcttacat
     1741 agtccattaa catattatat cacttcctac tcatttacta ttactactgc atgtgcaaca
     1801 catggtattg tctgcttttg tagtgtactt gtcggtgaaa gtagtgctcc tgttgataca
     1861 agcattactt tgttaaattc tttttttctc aacttatcat atatatatcc tgcaaacact
     1921 gtagctgaac atccacatcc acttccacca caatggacat cttgttcttt aaggttaaac
     1981 atctctatac cacagtcagt gcaaactttt gatatatcta cacccttttc ttttaaaaag
     2041 tcttctgcta tctgttttcc aagagtacct aaatctccag tagcaattat atcaaagcta
     2101 tttgggtcat cattagtatc ttcaaagtaa gagtagatag tatctattgc tgctggagcc
     2161 atagctggtc ccatattatt accatcatca ataccctcat ctattacttt accaacagta
     2221 acatacttta cttttggacc gtctccatta ggagctaata atacacttcc tgctcctgta
     2281 actgtccact gagctgtcat tggcttttga tttccaagtt caagtgggaa tctaaattgt
     2341 ctttctgctg tacaatagtg acttgatgta cctgacagta ctaaatcagc aaaaccacca
     2401 tctataagca tagaacctat acaaagacct tctgccattg tagaacaagc tccatatata
     2461 cctaaaaatg gaatagccag ttctcttgct gcaaatgatg ctggaactat ttgatttatt
     2521 aaatcgccac ctagcatata atttacatct gacaattttt tacctgcctt ttggatagct
     2581 tgttgagatg cagtctctac cattttactt tcagctaatt cccaagtctt ttcaccatat
     2641 aaatcgtcag tcataatcat atcaaaatag tcctttagtg gtccttcacc ttccttagga
     2701 cctactatag ttccagttga tattatggtt ggtttatttt ctaacttgac tgttcttttt
     2761 ccaattcttt tatttttcat attttcacac ctttttaatt ttgcatttta ctattcaaat
     2821 ttttaatatt aattaaaaat attaattaaa tctgcacaat tatttttcaa ttagttactt
     2881 tagtagctat tttcagattt tataccttct tcataattaa taaaatttaa attaaataaa
     2941 aatcatctaa aacatcttaa aaatataata aataattcca cataaaatag aagaacctat
     3001 accataaact aaaactggtc ctgcaatttt aaataagttt gcacctacac ctaatacata
     3061 gccttctctt ttatactcca ttgctggaga tactattgag ttagcaaaac ctgttattgg
     3121 aattatagaa ccagctccag ctctttttcc tattaggtca taaactccta atccagttaa
     3181 aaatgcccct ataaatatta atgttattga tgttgcagaa ctagcaccta gtttatcaag
     3241 acctccaact ttcatataca aatcatttat tgcttgccct atcatacaaa taattccacc
     3301 tacaataaat gcaagtgtat agttttttaa atatgtaggt tttgggctta tttggtctac
     3361 atatttttta taatttttat ccatactaaa cacctctcta aagtattatt tgttaatttt
     3421 aaaaaaataa acctattatt tattttgact atctaaagtt tggtcaaaaa ataaaaaaga
     3481 tgcctaataa agaaaaaatc tatttttcca taaagacacc ttgtttttat atttttactt
     3541 tttt
//
LOCUS       DACXMZ010000296         3491 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.297, whole genome shotgun sequence.
ACCESSION   DACXMZ010000296 DACXMZ010000000
VERSION     DACXMZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.297"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1130)
                     /locus_tag="JD016_18135"
     CDS             complement(<1..1130)
                     /locus_tag="JD016_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAU5001538.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            complement(1517..3454)
                     /gene="htpG"
                     /locus_tag="JD016_18140"
     CDS             complement(1517..3454)
                     /gene="htpG"
                     /locus_tag="JD016_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAU5001539.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
BASE COUNT         1089 a          628 c          344 g         1430 t
ORIGIN      
        1 tataaagact tatggtacat ttcctttatt tgttccttat taaaatactt tggatgtctc
       61 ttaatatttg caactgatat tttcatacaa ttatcagtta accaatctat atctgatttt
      121 tcaataccta aatcacttag ttttatatct aaatctattt ttttaaggaa atttctaatt
      181 gcatctgaac aatcttctgc acaagttcct cctaatacct ttgaaatatt accaaatctt
      241 tcaatatcac tatcccatga actttctata atagtaggag tcaaagctgc aagtcctttt
      301 ccatgcatta catctctaag accacttaca ggatgctcca ttccatgtgg caaggctgta
      361 cccgctgtac caatcgtcat tcctccaata gtacttgcaa gtgttatttt ttcccatgct
      421 tctaaatcat taacatcatt atataccttt attaggtttt ctgatataag ttcaatccca
      481 taaatagctt gtacatctgc aagagggttt cttccattag aaagatatgc ttccatatta
      541 tgggcaagtg catcaaatcc aactgatgca attatatgtt ttggcattgt aaccataagt
      601 tctgggtcaa ttattgatgc ttttgcatac atactattct tctttataga ttttttatct
      661 ttagtttctg aatttgtaag tactgcaaaa caatttcctt cactacctgt acctgatgtt
      721 gttggaacta agattatagg taaagcttct tcaccttgtc taattccaaa tatataatca
      781 tttaaatctc ctgggttttt ataagaaaaa gctatactct tggcagcatc cataatgctt
      841 ccaccaccaa gccctaaaac acaatcacat ccagttgatt ttataatttc tactcctgaa
      901 tatatagtag ttataagagg attttgttct actctatcaa aaacttcata ttctatttta
      961 gagtctttta gtaaatctat tgttttatca agtaatccag ttttctttgt actattttta
     1021 cctgttacta tcaatacttt tttgccatat ttagatactt cttttcccac atcattaatt
     1081 ctgcctctcc caaataagat atttactggc aaattataat taaaattcat tctaaaacct
     1141 cctatttcta ctttagtaat aattttatgt ttttaacaaa gtgtaattat gtatttaaac
     1201 ataaactaaa aaacaatctt ttacttaatt atatgtatta tcttattata tatcaagtta
     1261 actatattta actaaataga gttttttatt agaacctact taaatattta aaattaacca
     1321 aagataaaaa aataaagaac ggtctgtctc aaaatagaga taaatatatt tttcattgtt
     1381 gaaatgatta aaataaattt aatttcacat aaaaaaagcg cctcatgaac tgaaaagttc
     1441 attttgagac acttctttct catcactgat attgaatact ttttataaat ttgcttcaat
     1501 gtattcaaat tcaatcttat aattctatca aaagactcat aagcttatta cttctttgta
     1561 caaatgaatc taactcatct ggctttaatt ctttattaga aagtaaagct aaatctgcta
     1621 tctggttaca aataagcgta atcttatctt tcttttcctc atcatcttta agagaaacca
     1681 attttttgat tattggactg ttctcattta ttacaagagt tttttcttcc tcaaaattca
     1741 ttcctaaatc cattccagca aatcttgact gcatttctgc cattctaata gattgttctg
     1801 atactaatac catagctgga gtgtcttcat ttttcaatcc ttctacagaa taattcttca
     1861 ctctttctcc gacagcttct ttaaacagac cttctatctt agctatctct tctttgtttt
     1921 cttcactatc tttattttca tttttatctt ttaatacatc agaaaggtct gcatctattc
     1981 tattaaactt aactccattc tctttatatt ccataaatga aataaaatga gtatctattg
     2041 atgagtttaa aacaacagca cttaagtcgt attctttaaa tagtttaatg tattgagatt
     2101 gctgttcctt atcacttaca taaaatacct tattttcatg tttgtcctta catttttcta
     2161 aataatcttg taatgttata tactcatcat caatagtttt gaataatata cattctttta
     2221 ccttctcata gaaactttca tcttttaagc aaccatattt aataaatatt tgtatatcat
     2281 cccaaaactt attgtattct tctctttcat ttttacaaag agatttcaac ttatcagcta
     2341 cttttttaat tatatgttta gaaattttgc ttacatccct gtcattttgt aagaaacttc
     2401 ttgatacatt taatggtaag tctgggcagt ctataactcc ttttaataaa agtaaaaatt
     2461 ctggtataac ttcttttata ttgtcagcca caaacacttg attgttgtat aattttactt
     2521 ttccttcagt cagttcaaac tcatttttta atttagggaa gtaaagtata ccctttaaat
     2581 tgaaaggata atctacattt aaatgtatcc aaaataaagg ttcatcaaat acattgaata
     2641 cctttctata aaactcttta tactcctcat cagtacagtc ttttggagat tttaaccata
     2701 gaggattagt gtcgtttaaa ggctcaatta cttttgcatc tacaatctct tcttttccat
     2761 cttccttttg ttttttagct ttttcttctg cttctttagc ttctctttct aatctttcaa
     2821 catcttctaa atatatttct actggtaaga aagaacagta tttatttatt attcctctta
     2881 cagtaaattc atctaaaaat tctttactat catcatcaat aaatagtgtt atagtagttc
     2941 ctctatcatt tctagcatct gattcagata tttcgtattc tgtaccacct tcagatatcc
     3001 atcttacagg agtcgctcct tctgtatatg aaagtgtatc tatttgtacc tttttagaca
     3061 ccataaatgc agaataaaat cctaatccaa aatgacctat tatatcattt gactcttcca
     3121 ttttatcctt atatttatta aagaaatctt cagcacctga aaaagcaact tgatttatgt
     3181 atttttttat ctcttcttca gtcataccaa ttccattgtc aataaattta agtgtacctt
     3241 ctcctttatt tacagatact gtaattttat aatctgatga cttattttct gatatttctc
     3301 ctagtgatac aagtctttta tgtttactta ctgcatcaca tccattactt ataagctctc
     3361 ttataaatat gtctttatca gagtataacc attttttaat tataggaaaa atattttctg
     3421 tatgaattga aatactccct ttttcaaatt ccatttttta tccctcctca ttttataatt
     3481 aatattttaa a
//
LOCUS       DACXMZ010000297         3421 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.298, whole genome shotgun sequence.
ACCESSION   DACXMZ010000297 DACXMZ010000000
VERSION     DACXMZ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.298"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            56..1048
                     /locus_tag="JD016_18145"
     CDS             56..1048
                     /locus_tag="JD016_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAU5001540.1"
                     /translation="MELKDRSLVNNQYKGKIKSANLSVDYEINQVMKDIDKLGLNTVN
                     VPIIINVESINSDIMSIDNNSKEKAIKLIKKLNKKGISTILEAYPWIENGKLYETDWN
                     PINKKRFFYTWQNAILNELIIDVANPLDVNVLNIGSNFVHLEEYQQNWGEIIDFVQSK
                     FDGLVTYRTNWWYTKKDDLSSKLFYEQKLNNNFFDKLDFISIAAYFELSNKPVNTVDE
                     LISALHSSTVNNRGQNIKQEIYNLYKAHRKPIFFGELGFSNRESASSQPWNHTPSKVV
                     NGEEQARCFEAYKKVFENEDWINGFSVFCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            1102..2226
                     /locus_tag="JD016_18150"
     CDS             1102..2226
                     /locus_tag="JD016_18150"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAU5001541.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2304..3278
                     /locus_tag="JD016_18155"
     CDS             2304..3278
                     /locus_tag="JD016_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAU5001542.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         1428 a          333 c          597 g         1063 t
ORIGIN      
        1 tattttaatc tcattaataa tcttaatttt tatatttata gtttactttg taagtattga
       61 gttaaaagat aggagtctag taaataatca atataaaggt aagattaaat cagccaattt
      121 atctgtagat tatgaaataa atcaagttat gaaggatata gataaattgg gattgaatac
      181 agtaaatgta cctataatta taaatgtaga gtccattaat tctgatatta tgtcaataga
      241 taataatagt aaagaaaagg caattaagtt aataaaaaaa ttaaataaaa aaggtatttc
      301 aacaatatta gaggcatatc catggattga aaatggaaaa ttgtatgaga ctgactggaa
      361 ccctataaat aaaaaaagat ttttttatac ttggcaaaat gccatattaa atgaacttat
      421 aattgatgta gcaaatccac tggatgtaaa tgttttaaat attggttcta attttgtaca
      481 tttggaagaa tatcaacaaa attggggtga aataatagat tttgtacaat ccaaatttga
      541 cggtcttgta acgtacagaa ctaattggtg gtatacaaaa aaagatgatt taagtagtaa
      601 gttgttttat gaacaaaagt tgaacaataa tttttttgat aagttagatt ttatatctat
      661 agcagcatat tttgagttaa gcaataaacc tgttaataca gtggatgaat taatttctgc
      721 cttacactca tctactgtaa acaatagagg acaaaatata aaacaagaaa tatataacct
      781 ttataaagct catagaaaac ctatattttt tggagaatta ggtttttcaa atagagaaag
      841 tgcaagttct cagccgtgga atcatactcc ttcaaaagtt gtaaatggcg aagaacaagc
      901 aaggtgtttt gaggcatata agaaagtttt tgaaaatgaa gactggataa atggtttttc
      961 tgtgttttgt gtaggtaaaa tagatgacga aaaaaatttt tacccatcaa aagagagtat
     1021 aaaagttatt aagtcttggt atgaataatt ttgaaagtgt agaatttata tagattaaat
     1081 agatgattag gagagatata aatgaatgac atcaaggtta tgactgtatt tggaactaga
     1141 cctgaagcta tcaaagtagc acctttaata aaggaacttg aaaaaagaga gaatataaaa
     1201 agtattgttt gcgttacagc tcaacataga gaaatgcttg accaagttat agaaaccttt
     1261 aatataaatg tagactatga tttggatata atggaaaaag gacaaagcct gaatgatata
     1321 acatgtaaga ttttaaataa acttcccttg attttaaaca aggaaaatcc aaatataata
     1381 ttggtacatg gagatacaac cacaactcta gctacaagtt tgactgcatt ttataataaa
     1441 actcttgttg gtcatataga agctgggctt agaacctatg ataaatattc tccttttcca
     1501 gaggagttaa atagacaact tacaggtatt attgctgata tgcactttgc tcctactaat
     1561 ttggctagaa aaaacttgat ttctgaagga aaacctaata ataatatatt tgtaactggt
     1621 aatactgcta ttgatgcttt aaagatgaca ataaaagaaa actataatca tcctataatt
     1681 gatgagattg gtaacgatag gatgattttg ttaacctctc acagaagaga aaatttgggt
     1741 aaacctatga aaaatatatt cagagctata aagcgaatag tagatgactt tgaagatgta
     1801 cagatagtat atcccataca tttaaatcct aaaatacgaa ctatagctga tgagatattt
     1861 ggaaaatttc ctgaaaaaat tcatattata gagcctttag atgtagctga ttttcataac
     1921 tttttaaata aatcttatat gataatgact gatagtggag gaatacagga agaagctcca
     1981 tcattaggta agccagttct tgtgttgcgt gataaaacag aaagaacaga aggaattgaa
     2041 gcaaagacac ttaaattagt aggtactaat gaagatagaa tatataattc agtgagcgat
     2101 ttattaataa ataaagataa ctatgttcag atgagtaaag catctaatcc atatggagat
     2161 ggaaatgcaa gcaaatatat agtggatata ataattaaaa aatttaattg caaatattta
     2221 aattagataa gggctaaaac attaactaat tgatataaga tttatgataa tattaaggaa
     2281 tacaattttt aagggggagt aatatggcta gaaaattaat aaaaaatttg ggtaaaagta
     2341 aaagtgtaaa gagagtgaaa cttttattta agaagatttt tattactgta ttcatagtag
     2401 caagtatagt tgctattttt aatataacga aatattttga ggaattatac aaagtaaggg
     2461 atttaaaaag tactaaaatt gaatactata tggatgtggc tgatgaagca ggagatggaa
     2521 aggtccaatt aagttggaag gccttacttg ctatagacat ggtaatacat gatgaagatt
     2581 taagtaatat aaaaaagaaa gacacattgg atatagggga aaagtttata gtagaagata
     2641 aaaatgataa aggcgaaaag gtgtataagg taaaaaagtt taataaggta ttaagcgaat
     2701 tgaaatttga ctcttctcaa aaaagtagag caagaaaata catgaaagat ttagaataca
     2761 cataccttgg aaataaacaa ttagatagta gtgatgaaaa aattaaattt ataaagaagt
     2821 tagaagactc agctataaga gaatatattg attatggaat attgccctct ataacaattg
     2881 gacaagctat attagaatct ggttggggaa attctaaact tacaaaacag agtaataatt
     2941 tatttggtat aaaagcagat aaagcatgga aaggaaaaag tgtagaaatt tcaacttcag
     3001 agcattataa tgaaaaaatt gtagctagtt ttaggtcgta taattcatta caagattctg
     3061 tcaaagacca cagtttattt ttaattaata ataaaagata tagaaaacat gggttgtttg
     3121 aggcaaaaga ttatattagt caagctcaat cattagaaaa tgcaggatat agtacagcag
     3181 aagataaaaa aggaaatcgt atatatgcag agctattaat agatgttatt aggagttata
     3241 atttgcaatt gatagataat aaagttgaga caaaataatt gttaacttta tatgaggaga
     3301 aaacaagata agattctttt ctacaaaaag aatagaaaaa ttgtaaaaaa aactaaaaaa
     3361 atgtattgac ttgttattta aaaggtgata ttattattac tgtactcgaa agagaacaaa
     3421 a
//
LOCUS       DACXMZ010000298         3399 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.299, whole genome shotgun sequence.
ACCESSION   DACXMZ010000298 DACXMZ010000000
VERSION     DACXMZ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.299"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..198)
                     /locus_tag="JD016_18160"
     CDS             complement(<1..198)
                     /locus_tag="JD016_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HAU5001543.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFF"
     gene            complement(202..1338)
                     /locus_tag="JD016_18165"
     CDS             complement(202..1338)
                     /locus_tag="JD016_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HAU5001544.1"
                     /translation="MEEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRVP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(1370..1915)
                     /locus_tag="JD016_18170"
     CDS             complement(1370..1915)
                     /locus_tag="JD016_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAU5001545.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            2239..3009
                     /locus_tag="JD016_18175"
     CDS             2239..3009
                     /locus_tag="JD016_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001546.1"
                     /translation="MKVNENNIDEELDELSRKVYLESPDFWISISDKIIDKVFDEMLL
                     FFAIKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYLLSL
                     NKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDITYKF
                     SSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGIIAK
                     KKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
BASE COUNT         1215 a          527 c          378 g         1279 t
ORIGIN      
        1 aaaaaaacta atcaactgaa acattatatt tttagaatat attaaaattg ggatattttc
       61 acctaaagaa atttgttttc ctagttcctc actataactc aatatatttt cataagtata
      121 tttttctcca tctataatta aaaaattttt atttttatgc ctctcttttt ttattcttaa
      181 aagttcataa tagttcataa tctacaccct ttcaagcagt aaagctgaac ctagaccacc
      241 agctgcagct atagagcaaa ttccatatct acccttagtc ttttctaatg cttttaaaag
      301 atgtaatgca ataattgcac ctgaagcacc atatggatgt ccatacgcta aagctccacc
      361 aaatatatta tatctatcta ttaactctgg atactttctt tgaaacaaca catctataac
      421 tgcaaatgct tcattaaatt caacagcaga tacatctaaa taattaagac tttctatctc
      481 taaaagttta tccattgctt gtatcgctga tgttggactt aaggttgcat ctgttcctat
      541 tgtgcatgta ttaataattt ttgctttagg cttctttttt acatgttcta aatacttctt
      601 agaacatatt ataataaatg aagctccatc attaataaga catgaatttg ctgcatttgt
      661 aatagtttct tttcctaaaa ttgatggcac cctatctaat aacctttgac tcatcttatc
      721 acgtatacct tcatcatagg tactattatt tataggagat ataatatcct caagtatttt
      781 ttcttctctt gcttcttttg ctcttttatg actttccttt acccaaaaat ccaagtctgc
      841 tttctcaatt tgatatagct ctgctactct ttccgcccct tcgagcatac tattttggct
      901 attatcatca ggagaaaatt gagcaactgt ataatttgga ttatttgtat tatatcgctt
      961 atcattctta tgatatgtgc gcattggttg tagagaacta ctttcaaaac cacctgcaat
     1021 aattaaatca cactgaccag atttaacctt tgaaaaagct atgtctatac tcatcatagc
     1081 agaagcacac tgcatatcca cagtaaaagc aggtacttca tttgacactc ctgctgttaa
     1141 agtcattaat ctagctatat tccctcctgt cccaactgca tttccacata taatctcatc
     1201 aattttatca atttcatact ttttaattaa atcttttaaa acacttgctc ctagtaattc
     1261 aggttgaaca aattgaaaaa ttccattttt tagacctata tgacttctca atcctcctaa
     1321 tataaaaact tcctccatac attacaccct tccttatatt atagatttat catagatttc
     1381 ttctgagtac attgttcagt ttagtgccta aaaaagatgc aataaccgac tttattgtat
     1441 ctccaattaa aaatggtact actgctgcaa aaagtgctgc ttctactgtc atttgattaa
     1501 aatagcacat aaatactgtt cccataaaat atataatagg cattccaaca aaaattgtta
     1561 ttagtatata tctcttaata tcattatttc gtccttttaa taaactaata attagtgctg
     1621 caaacaaaaa cccaaaataa aaaccacctg ttgggcttaa caactttcca ataccagatg
     1681 tacctcctcc aaatactgga atcccaatta aacctatcat tatatataca ccaactgtac
     1741 taaatgcttg tcttggtgtt aaaatcaaag ctattaaatt tatcattatt gtttgtgctg
     1801 ttactcctgc tggagtaaat ggcaatggta tatatatgta agatgatata cacaataatg
     1861 ctacacatat tgacatttta gttaaatctt gaatatttac tttagtattt ttcattttta
     1921 taattccctt catatgtctt atttcattta gtttatatgt acatatacaa agcttctatt
     1981 aaagctttgt tttagtataa aataaagacg tttttttgtc aactatcata agtatttagg
     2041 tttacattta atttataagt aaactttata ctgttacttt atattttaat agtcatttat
     2101 aaaactctgc ttaaaaacag ctctatttag actatacttg taaattataa tatgatatat
     2161 ttttatataa gattttattt tatataaaaa atagtaacaa atagtttata cacaagatat
     2221 ttaaaaggaa gtgatgatat gaaagttaat gaaaataata tagatgaaga gttagatgaa
     2281 cttagtagga aagtttactt agagtcccca gatttttgga taagtatttc tgacaaaata
     2341 atagataaag tatttgatga aatgttatta ttttttgcaa ttaaatataa ctatttcaat
     2401 atagtatgct ttgctattga aaacaattat ctggatttaa acatgccttc aaaaaacaaa
     2461 aaatacacta acataatgga gcatttgtta gatacatcaa aacagaatga agataaaaaa
     2521 atttataatt atcttctaag cttaaataaa ggtaataacc tagatgaaag accaatttta
     2581 gatgaattaa actgtaatat tagtagtgat acttttgaaa aaaaagatat atatcttcca
     2641 aagtacttat gtccaagttg taattctaat atatttgata caggttataa agtatcaaat
     2701 gacataacat ataaattttc ttctaaagaa gctgaaccta ttgaaatgtg tagtgaagtt
     2761 tcttctatca gatgttgtaa ttgtgatgaa ttattagaag atattacact tgataaacta
     2821 tataacctat gcaaaattca aaactgtaat aattgtggtt ctaatttaac taaggtagga
     2881 attattgcaa agaaaaaaat gaactttgat gagaatactg aaaaatttaa agatgtctca
     2941 acatcttact gttgtggaaa ttgtgactct gaaataaact tatctcaaag agaacatttt
     3001 aagatttaaa tatttatagg ctaaagtaat aagattacat tattagatac tgtataagtt
     3061 ttttaaatct gtaaaaataa atacacttat aagaattaaa attgctatga aactagttct
     3121 tgatgaacta atttcatggc aattttctat aatctaataa ttattaaatt ttaacaaacc
     3181 tttattagca ttccataatt atttaaaatt ctttcaaata ccctatagtt tataggtgaa
     3241 agtatcttta atattatatc aaacttatta tctttaaata gtaaatttgt taaatctgaa
     3301 atacaccata caattttatt ttcctctttt gtagctatat caattctgat taacttacat
     3361 ctataataaa tatctttatc ttcatatagt ctattttaa
//
LOCUS       DACXMZ010000299         3360 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.300, whole genome shotgun sequence.
ACCESSION   DACXMZ010000299 DACXMZ010000000
VERSION     DACXMZ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.300"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            107..634
                     /locus_tag="JD016_18180"
     CDS             107..634
                     /locus_tag="JD016_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAU5001547.1"
                     /translation="MERRCNMNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIV
                     QDIALIRARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIK
                     DVIVNHPTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEET
                     IEAIVKELKDNGILS"
     gene            646..1527
                     /locus_tag="JD016_18185"
     CDS             646..1527
                     /locus_tag="JD016_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HAU5001548.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     misc_binding    1655..1860
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1986..3257
                     /gene="serS"
                     /locus_tag="JD016_18190"
     CDS             1986..3257
                     /gene="serS"
                     /locus_tag="JD016_18190"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAU5001549.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
BASE COUNT         1375 a          319 c          587 g         1079 t
ORIGIN      
        1 tcttttttta tttattataa aagttaagat taaatagaat ataaagtcaa tattgattta
       61 taataagata aaatataaaa ttatatagta taggtaaagt ttttatttgg aaagaaggtg
      121 taatatgaat tctaatgaaa gaagaagtaa attaatagat attttaaaag aatctaagca
      181 tcctgtaaaa ggtggaacac tagctgaact attaaatgta agtaggcaag taatagttca
      241 agatatagcc cttataagag ctagaggatt tgaaataata gctacacctc aaggatatat
      301 aatatataat caaccgtatt ttacaaaaca aataaagtgt aaaaatcata aggattctaa
      361 agaaatttat gatgaattaa agataattgt agatttaggt ggtattataa aagatgtcat
      421 tgtaaatcat ccaacatatg gtcagataac tgcagaactt aatatttatt ctaaaatgga
      481 tatagacaac tttatgaaga aagttgagac aaatgaattt aaacaattat cggttctgac
      541 agaatgtagc catattcata caattgaggc aataaaagaa gaaactatag aagctatagt
      601 aaaagagtta aaggacaacg gaatattatc ataaggagga gttgaatgaa ttttttatca
      661 ttggctaaga ctgcctcaac aaatagcaat ataactaaga gaactttaga aaatatgctc
      721 gataatttta gtgataatat accaaatatt atatatgcaa taattgtatt tgtgataggt
      781 atttatatat ctaaaatagt aagaaggatg gtttcaaagt ttctaatcaa atatggaatg
      841 agtaaaggtg taaataattt tattgtatat ggaatatata tatctatgtt atctattata
      901 tcattgataa gcttaggtat tatagggata caaactacat cagttgtagc tgtgttagga
      961 gctgctggat ttagtatagg tttagctttt aaagaaatat tatcaaattt aggctctggt
     1021 atgattattt tattttttaa gccatttaat ataggtgatt atattcaagg ttcaggtgta
     1081 gaaggtacag tttctgacat acaaatattt agcactgttc ttaagacgcc agataataaa
     1141 actataatta tacctaattt tcaactgaca agtaataata taattaatta cacacatcaa
     1201 aacaaaagaa gaatagattt ttcatataat atttcatatg atagtgatat agatgtagtt
     1261 aagtctatat taaatgaaat ttttacaaac gaaaaaagaa tattaaatga tccaaagcct
     1321 ataataggtt taaactctat aggaaataac accatgcaaa tagttgctag accttgggta
     1381 aaaactgatg attactggga tgtatacttt gatgtaatgg aaaaggtaaa aaataaattt
     1441 gatgagaatg atataaaagt tccatttgtt ccttcttcat tattatttaa taatacaggt
     1501 aatctaaata caatggataa aagataaggt cttaattaaa tagaaaaaat gaatttaata
     1561 taaaaaatta aagaaaattc taaaaaaatt tgaataatag tattgaaaga tgttaatata
     1621 tataatataa tgattatcaa ataaaacaat tagttaaaaa gctgtgaagg aaaatagtaa
     1681 aaaacagatt gtattcagag aatgagtgga tggtgaaaac tcattacaat ggtttttgaa
     1741 tctactctag agcttctagt taaagctaga cgtttaccta cgttataggt ctgagaggat
     1801 tatacatttt agtatagtca attagggtgg caacgcggat aaaagatttt cgtccctttt
     1861 atagggtaaa cggaggtctt tttttatttt taaaatttgt ttaaactaag agtttacaag
     1921 tataatgttt gtaaattaag ttggaattat aacttatatt atcaaaatta ttaggaggaa
     1981 ttaatatgtt agatattaaa agaataagag aaaatctaga tgatatcaaa aaagctatgg
     2041 aaagaagagg tgaaagagaa tttgaccttg atgctgtagt ggaattagat aataaaagaa
     2101 gagaaatact tcaagaagtt gaagttatga aaaatgagtt aaatgtaaat tctaaaaaaa
     2161 tacctcaatt aataaaagaa ggaaaagatg taacagaaga aaaggcaaga ttgaaagagt
     2221 tatcagataa gataaaaggt attgatgaga aagtaaaaga agtagaagca aaaatggaat
     2281 atacattaat gagaatacca aatgttcctc atccagaggt accacaaggt gaaacagatg
     2341 aagacaatgt tcaaataaga acttggggag aacctactaa atttgatttt gagcataaag
     2401 cgcattggga tataggtaca ggtctaggta ttttagattt tgaaagagca gggaaaataa
     2461 ctgggtcaag gtttacttta tataggggat taggagctag attagaaaga tctttaatga
     2521 atttcttttt aaatactcat acagctaagc atggctatac ggaagtctta cctccattta
     2581 tggccaatag aaatagtttt atagggactg gtcaacttcc taaatttgaa gaagatatgt
     2641 ttaaaataga aggattagag tatttcttaa taccaacagc tgaagttcct gttactaata
     2701 tacatgctaa tgaaatatta gatgttgcag agcttccaat taaatactgt gcatatactc
     2761 cttgttttag atctgaagca ggttcagcag gaagagatac aagaggttta gttagacaac
     2821 atcaatttaa taaagttgaa ttagttaaat ttgttaaacc agaagattct tataatgaat
     2881 tagaatcatt aactcatgat gctgaaacta tgttacaaat gttaggtctt ccatatagag
     2941 ttgtaaaaat atgtacagga gatttgggat ttacagctgc atttaaatat gatttagaag
     3001 tttggatgcc aagttacaat agatatgtag aaatatcttc ttgttcaaat tttgaagatt
     3061 tccaagcaag aagagcagga ataagattta aaagagataa aaaatcaaaa gcagaatatg
     3121 ttcatacatt aaatggttct ggattagcga taggaagatg tttggctgct atattagaaa
     3181 attatcagca ggcagatggt tctgtagtag ttccagaaga attaagacct tatatgggtg
     3241 ttgatgttat aaaataaatt tatattaatt gtatatgtag agtatgcttt gatgaagaga
     3301 atatattatg agtaaacaat aaataaaaaa actatgaaat tattataaat attaatttca
//
LOCUS       DACXMZ010000300         3291 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.301, whole genome shotgun sequence.
ACCESSION   DACXMZ010000300 DACXMZ010000000
VERSION     DACXMZ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.301"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            244..1512
                     /gene="eam"
                     /locus_tag="JD016_18195"
     CDS             244..1512
                     /gene="eam"
                     /locus_tag="JD016_18195"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HAU5001550.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(1605..1961)
                     /locus_tag="JD016_18200"
     CDS             complement(1605..1961)
                     /locus_tag="JD016_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001551.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(2112..3206)
                     /locus_tag="JD016_18205"
     CDS             complement(2112..3206)
                     /locus_tag="JD016_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAU5001552.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         1348 a          433 c          468 g         1042 t
ORIGIN      
        1 taaaaataga aaaaattaat catttgttaa aaaaaatgca taatttattg taatttattg
       61 acgaaggttg aaaaatattc ataaatgtgc gaaataacag aaaaaatgca aaaattaatt
      121 caatttcatt tgttataaaa tctatatggc aaaattaaat ctgcctatca gaatgatata
      181 aacacatcag aaaggaggac gctatcaaat aatacaagta gttaactttg atagcaaaaa
      241 attatgaatg aacaaactag aatatcctta gagagagctg ctgaattaaa atcaaaaatt
      301 gatgattata ttcaggctag aaaaacgatt aacagaggtc ttgaaaaaga agaagagata
      361 aataaacgaa aacagaaaat attaagtatc ttaaatggaa ctgaagagga ttggaataac
      421 tacaaatggc aattatcaaa tagaataaca gatgtagata ctttatcaaa aattataact
      481 ctaactaaaa aagaaaaaga atatataaaa gaggttggta ctcaatttag atgggcaata
      541 tctccatatt atttgagtct tatagaccca gaagatatat gtgacccaat aaaattactg
      601 tctataccaa cacatataga gttggaagat gaacaagaag atttggaccc aatgggagaa
      661 gagtatacaa acccagcagg atgtataact agaagatacc cggatagatt aataataaat
      721 gtaacaaatg agtgtgctat gtattgtaga cactgtcaga gaagaagaaa tattggacaa
      781 caagattctc ataagtcaaa agctattatc caagaatcta tagactatat cagagaaaat
      841 gaagaaataa gagatgtact agtaactggt ggagatgctc ttacattaaa agatgattat
      901 ttagagtgga ttcttagcca acttaaagag ataccacatg ttgattatgt tagattaggt
      961 actaggactc ttgttacaat gccacaaaga atcacagatg aattttgcaa tatgctaaaa
     1021 aaatatcacc ctgtatatat aaatactcat tttaatcatc caatggaaat aactaaggaa
     1081 tctaaagaag cttgtgaaaa gttagcaaat gcaggagttc cattaggaaa tcaggcagta
     1141 ttattaaatg gaataaataa tgataaattt gtaatgagat gtttaaatca agaattactg
     1201 aaaataagag taaaacctta ttatatattc caaagtaaac atgtaaaggg aacaaaacat
     1261 ttcaatacat cagtagatga tggtcttgaa atcatggagt atttaagagg atatacatca
     1321 ggaatggcta taccaacata tatagtaaat gctccaaaag gaggaggaaa gactcctttg
     1381 cttcctcaat accttgtatc aaaaggaaca gattacgtta tgcttagaac atgggaagga
     1441 aaagttataa aaatggaaga tgaacctgct gtagatataa agaaacttat aaaagaacaa
     1501 gcacaggatt aaaaaaatat ttaaagttta taaaaagaaa caactttaaa ataaaaagaa
     1561 aatggatgtc aaatttatat caatttgcat ccatttttta ttagttattt attattttgt
     1621 tgttgattaa ctaattcaag atatccactt gggtggaagt aatatatctc agaagtattt
     1681 tcatttacaa agtaaccaat atcaccatct gcatctggta aaaatacata accattaaga
     1741 tttttagctt ttaatgcttc aaaatcacta gcatttcctt gatatatgta atcaacttca
     1801 ttgttgtatt ttactaaaag ttctttagct tgttcttgag tcaatttagt attttggctt
     1861 acaggaacat tcttttgaga atctgcataa gaagattcat ttactggtgc agttaataac
     1921 aaacaagata aaactaataa agatataagt gcttttttca tactaaaacc ctctttcatg
     1981 tttaattata taagattcat tacttaataa tattgtatgt ttcctataat taaatattac
     2041 cattaaagag aataatatta cagttacaca gctgtaactt ttgattacaa aatcgtaatt
     2101 aaattgtatt tttattccat ttgtatttta tcatgtctac gatttacaaa ctcacagaag
     2161 tatatcttta taacgcccat cacaggagca gatagaatca taccaggtat accaaagaat
     2221 cctccaccaa cagtaactgc taggattgta aagaatgggc taagacctag ttgtccacct
     2281 acgatttttg gctctataaa tgtaacttca acttgttgaa ttattattaa gtaaattaaa
     2341 gaagttaaag caattgttgg attggagaat aaattaatta ttacaactgg tgccatacct
     2401 attactggac caaaatatgg aaccatattc atgacaccca taagtgtacc aaataatagt
     2461 gcatattcag attttaaaaa gtaaagacca attccagaaa gtactccaac tataaaagaa
     2521 tctaaaatct tacctgtaaa gtatttacca atatttaaat ttaagctttg gcatagttct
     2581 attataaagt ctccatattt tttacctaag gaaatataaa ctactttttt tgtaaacagt
     2641 aagaattttt ctttttctaa taatatatag aaacaaatta taaaacctaa aagaaattgt
     2701 actataaatt ttccaactga aaatgtagtg ctaaatatcc caccaagtga accaataagc
     2761 aagttactaa atttaggcat tacactcata attttatcac catactcttt taatgtatta
     2821 gggtcaactg attgtaaact cttacctaag tcgaataaaa actgctctgt tttatctatg
     2881 tatgtaggaa tttgagcaac taaatcagct aaactattta ctatggatgg aactgtaaat
     2941 aatataaagg aaattagtat aactatgagt acaccataag taagaagtaa cgaatatatt
     3001 ctcttaaaat ttaatttact ttctaagaac tttactagtg ggttgaatat atatgctaat
     3061 acaaatgcta ttataaatgg tgttagtagt gacattaata aacttatcac tccaaaaaaa
     3121 tatttgtaat tatcaataac tttaatcaag atatatgaaa caactacagt gagtacaata
     3181 tttagataaa tttttttatc ttgcaaaata acacaacctt tcttatattt attaaattta
     3241 gatatactta aatctaagtt aaagttaata tttatagctt attatacaat a
//
LOCUS       DACXMZ010000301         3291 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.302, whole genome shotgun sequence.
ACCESSION   DACXMZ010000301 DACXMZ010000000
VERSION     DACXMZ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.302"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            68..1237
                     /locus_tag="JD016_18210"
     CDS             68..1237
                     /locus_tag="JD016_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAU5001553.1"
                     /translation="MNELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(1400..1942)
                     /locus_tag="JD016_18215"
     CDS             complement(1400..1942)
                     /locus_tag="JD016_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HAU5001554.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(2090..2374)
                     /locus_tag="JD016_18220"
     CDS             complement(2090..2374)
                     /locus_tag="JD016_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAU5001555.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(2437..3201)
                     /locus_tag="JD016_18225"
     CDS             complement(2437..3201)
                     /locus_tag="JD016_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAU5001556.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         1104 a          458 c          484 g         1245 t
ORIGIN      
        1 ttagtatgca aaattctgtg aagcaggaac cctgtgactt tagtcatggg aggttcaggg
       61 tagaaagata aatgaacttt gtaaagatga agttgtagag ttttgcaata gaataaaagg
      121 taaagtggaa agtgttgcaa taagtggagt atttgctcca gtaaataaac aacaagaaga
      181 agaagttgca aaatgggtta gagaaatttt aggggatatt cctatatctt tatcaaatga
      241 aataggtaat ataggtatat tagaaagaga aaatggagct attttaaatt ctgcattgtc
      301 tcaagtaggt aaagctgtat ctttaggatt tgaaaaagca cttaaagact taggtataga
      361 tgctcaaata tatttcagtc aaaatgatgg aacattaatg aatttagagt atacaatgaa
      421 atacccaatt tttacaattg gatgtggaat aacaaattct ataagaggag cttccttctt
      481 atcaaaagta aaaaatgcaa tagtattaga tgttggtgga actacaagcg acttgggtgt
      541 actatataat ggattcccta gagaatcatc tattccagtt acaattggag gagtacatac
      601 aaactttaga atgccagatg ttttaactat tggccttgct ggtggtacat gtgtaaaagg
      661 cgaaaaagat aatataaaga taggtcctga aagtgtggga tatagaataa ctgaagatgc
      721 aataatattt ggtggagaca ctctaacttt atcagatgtt gttacaaaat tagatatggc
      781 gtttgaagat agaaaaagca atgttgaaaa cttagataaa gagtattgta atgaggtata
      841 taagaaggtt attttaaaat tagaagatgc tgtagaccaa atgaagacta gtcaagatga
      901 tgttgaattg gttttaactg gtggaggaag tataattgtt cctgaaaaac taaaaggcat
      961 aagcaatgtt ataagacctc ctcattatac tgctgcaaat gctattggtg cagctttagg
     1021 acaaataagt ggagatgtag aaaaagtata cagtttagat aaaatgtcga gagaagatgc
     1081 catagaagat gcaaaaaaag aggctgtaaa taaagctcta aaagctggtg ctaaagaaga
     1141 tactatagaa gtattaaata tagaagatgt tccacttgca taccttcctg gtaatgcact
     1201 tcttataaaa gtaaaagttg ttggtgattt aatttaaaga ataaatataa gtatttccag
     1261 aattatataa atatttcttg actcattttt aaacttattt tatcaataaa aacagcataa
     1321 cttttgacta tattcttata agtttccaaa cagccgatat atacctattt tttgaggttt
     1381 atcggctgtt tgttatcttt tatcgcattc caactatttt tcttaccgta tttgctgtac
     1441 gaattgtaat cttatcactt acatttgaac tggcagtctt tgaccaccaa tttgtctttt
     1501 tataatcttt acgactaaaa gaccaaaaga tagcattttt ataatgatat actttttcat
     1561 actcttcttt aggatttcca acctcatcat aaaactcgtc ataagataag tcaggaaaca
     1621 taaaaataag attatcatat atttctttgt tgttatctcc ccaccaatta ggagcattat
     1681 ttagtatatc ttttaactct tcttgtgaaa taataaaaac tggtatttcc aattcaaact
     1741 gtttttttat cattaatctt attgtatttg agataacctt gctgtcatct acatcactta
     1801 aaaagacaac attaccacta ttaaggtatg tagaaacttc tgaataacca agttctacaa
     1861 agcttgcttt taattcagac ataattatct tgttcttacc acttatattg atacctctta
     1921 ataatgcgat ataacttttc atataataat ttcacctcta aacattcaca tttttctatg
     1981 tagtacaaac tggaatttgc atcttttata attctttcaa aacttcttca tatgtaattt
     2041 ttttaatata aaaaaatcgg tgcttaattt gtaccgattt ttgtttttct tagtaaagaa
     2101 ctcgtttttt tgcttctgct atagctctag cagcactttt tgcttgcatt cttttttctt
     2161 cttctttttt aaattcattt attgtttcat ttagattctt atcatgttca tttttttctt
     2221 tatcttcgtt ttctctgtat actaatatat aatctattat tgattcgttt attatttgag
     2281 atatactttt attttcatta attcctattc tcttaacttc cttaagtaga gtctcatcaa
     2341 tatgaaccgt tgtttgcttt gtattttttg ccaccttaat cacctcttaa aaaaaattct
     2401 aacattaaaa ttttttttag tcaataaatt ttaatattaa aattctttaa atgttttatt
     2461 tgattttcta aaattttcta ttgtatttat catcttattt tttatatttt cacctagtac
     2521 agaagacttt agtctaggat tttcgatatg gtcataaatt ggttctaaaa attcgacttc
     2581 gacaggaaca gagttaattt taccaatagg ttggtatcct tcataagtat cttttgaatt
     2641 ttttattact agtggaacaa tagggcattt tgctttatat gctatcttta aagctccact
     2701 tctaaactcc gaaaccaatg aatttggttc tttaatccat gttaaatctc cttctggaaa
     2761 cactgccata ctttgacctg taagtatatt ttgagaggct tgggctatac tctttatacc
     2821 ctcacgattg ttttttctgt ttacatatac acatcttagt aattttgccc attctttaaa
     2881 tataggtata tttctccaaa ctggttcatc tgcaattaca catccaattg gtctttctac
     2941 acttgctgtt agtatgaagc tgtcaagcat actagaatga tttacaacaa atagaagtgg
     3001 ttcttttggc agtgtttcct taccaattat attaagctct atgttcacta tatctaaaga
     3061 ttttttagct tgtttttgca taaattcaaa tttttccttt aagctaaatt tatcaggatt
     3121 ctttttaatt ttaataagcc taggtataga agatgtaaaa cctataagtt gatatgtagc
     3181 aaattttaag taattattca taaaattatc tccttaaaat aaaattattt attactgtaa
     3241 agattttaac ataaaataaa atttaagtct attttaattc aattttgtaa c
//
LOCUS       DACXMZ010000302         3276 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.303, whole genome shotgun sequence.
ACCESSION   DACXMZ010000302 DACXMZ010000000
VERSION     DACXMZ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.303"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            171..1403
                     /locus_tag="JD016_18230"
     CDS             171..1403
                     /locus_tag="JD016_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HAU5001557.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFATSYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            1417..2223
                     /locus_tag="JD016_18235"
     CDS             1417..2223
                     /locus_tag="JD016_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HAU5001558.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2335..3204)
                     /locus_tag="JD016_18240"
     CDS             complement(2335..3204)
                     /locus_tag="JD016_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAU5001559.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
BASE COUNT         1172 a          389 c          575 g         1140 t
ORIGIN      
        1 ggactattta tctatttata acttaaatta tatcaaattg ataacaaact agatattata
       61 cttttttgta gaatagaatt acaattatat aaagaaaaat tctattaata gtgaaaaaaa
      121 agtagaaaaa agtagaaatt attcttttat aaagaaaggg agggataata ttgcaagaga
      181 gagaagaatt agttttaaat ccagttccaa aagaggaaag agttggatgg ttagctccat
      241 tgtttaatat gcttggttct aacatagcta tatctgaact aatggttggg ggaactttaa
      301 ttttaggaat gactctttct aatatgatta ttacatcaat tatagggaac ttaatattag
      361 ttgcaattat tatgatacaa ggttatatag gttacaaaga agggttaaat acgtatgtat
      421 tagctaaggg ggcatttgga gagataggag gaaagtatct tatatctcta ttacttggta
      481 taactagttt tggatggttt ggtgtacaag caggggttgc aggactatca gtacaaaaga
      541 tattcccaag tgtaaaccta acattagtta cagttatact tggattattt atggttgttt
      601 ttgcactata tggctttaaa gctatggcta aatttaatta cttagttata ccaccactta
      661 tgatattaat ggtgtgggga gtttttaaag ctttttctac ttatggagtt gaagctattt
      721 ataattatac tcctcaaaca actatgagta tggttgaagg tcttaatatt gtagtaggtc
      781 ttgttatagt tggtgcaatt atatcacctg accagttaag atatacgaga cgagtaaaag
      841 atatttggat tatcagtttt ttaggtctag ggattatttc attattccaa caagttgcag
      901 ctggtgtaat gtctatggga gcacctactt gggatataac agaagtattg gctaatttag
      961 gttttggatg ggtagcgttt gttattttaa tacttgcatc ttggtcaaca aacgtatcaa
     1021 atgcttattc tggtggatta gcattaaaaa ctatttttcc aaatgtaaaa agaaatgttt
     1081 tgactttagt tgcaggtcta ataggtacaa taatagcagc tactggtata atatttaaat
     1141 ttcagtcatt tttatctttc ttgggaatcg cagttcctgc aatagcagga attatgtggt
     1201 gtgagtatta ttttatacaa ggaagaactt ataagcatag agaaggaata aattggattg
     1261 cagtaataag ctggttaata ggttttgcaa cgtcgtatta tagttcaaaa ataaatttct
     1321 taatacctcc tataaatgga attgtagtga gtatggttat ttattatata ttaatgaaat
     1381 gttttggaat caaagataaa taaagggggc aattacatgg gtcgtaaggt gaaaataggt
     1441 ataattcaac aacacagtgt acttggaaat gttaaaaaaa atatagaaaa agcagtagag
     1501 atgatagatg acttaggaaa acaaggggca gatataattt gtttaccaga actatttgct
     1561 acaggatata atttagagtc tttaggtgga gttaaaacat tagaattaat aagagaacat
     1621 aataagtaca tagaggaatc aatgtcagaa gctgctaaaa gaaataatgt atacctgata
     1681 tctccatatg gaaccttaga aaaagggtct acacatgttt ataattcagc agttatattt
     1741 gatagaaaag gtaagattat gggagaatac tgtaaaaatc atctgtggtc attagaagca
     1801 gtatacttta aaggagggga aaaagtagag gtatatgatg ctgattttgg tagatttggt
     1861 gtaatgatat gttatgatgc aggttttcca gaagtatcca gagaattgac attaaaaggt
     1921 tctgaaataa tatttatacc atctgcatgg agaatacaag atgaagatat gtgggattta
     1981 aatgtatctc aaagagcttt agagaacact gtatatacag taggtgtaaa tttagtaagt
     2041 aatgactcta atttaatact atttggaaag agtaaaatat gcaatcctag aggtactgta
     2101 ataactcaat tagatacata cagggaaaaa tatgtattat cagagattga tttagatgaa
     2161 ataaaaaagt atagggaaga tatcccctat ttgaaagaca gaaaaagtag ctataacata
     2221 taattctata ctataataca agttcaatat aaaatgataa aaagaggatg tatcaatatt
     2281 aagattaaat ttattatact ttgatatatc ctctttaata ttatcaactt tgatttaaat
     2341 atttttaaaa tagttgataa gaaagttttt gaacaacaac acagctttag attcatagtt
     2401 ttcgctaaac catgaaagat ttaaatatct tttaaattca atatctttga tatgtaacaa
     2461 cttaatctca gggtcttgag ataatcccca tgattttcca catataaatc caactccttg
     2521 aagtgatttt ataagagcat aaattgtata tggtgaatca gactcaaatg ctattttggg
     2581 tttgaaatta gctgattcgc acaggatatc aattacctct ctataattct cacccttcgt
     2641 tataactata aagttttcat ctgaaacttc gcttagatat acttcatcct ttaatgataa
     2701 aggatggttt attgaaactc ctaatagtat ttcttcgcat gttaaagtta tgctattttc
     2761 tgagtttagc tttgtaaatg aagaagatat gtacaaatca aaatcacttt ttttataaga
     2821 tgatggcaaa gtatgtgaca cattaaaagt aatattggga tatagttttc tgaaattact
     2881 caaaagtgat ggtaacacat tagccgctgc aggagataat atctttactt ctcctgaatt
     2941 ttctaaagaa gtgtcttgta actccttttt tgaactttct actaaatcta aaatgctatc
     3001 tactcttttt agaaacaatc ttccatactc atttaaaaca atatatcttc cttttctgtc
     3061 aaataactga actccaagtt ctttttctaa ggaagatata gttttgctta aagctggctg
     3121 tgctatattt aacttgtgtg atgcttttgt tatgtgctcc attctagcaa cagtttgaaa
     3181 atacttaaga tgcaataaat ccatagtata aaatacacct cttttaatat gacttgagtt
     3241 atatcaattt tataatcaac tccacattat acatta
//
LOCUS       DACXMZ010000303         3265 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.304, whole genome shotgun sequence.
ACCESSION   DACXMZ010000303 DACXMZ010000000
VERSION     DACXMZ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.304"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(55..627)
                     /locus_tag="JD016_18245"
     CDS             complement(55..627)
                     /locus_tag="JD016_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAU5001560.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(656..922)
                     /locus_tag="JD016_18250"
     CDS             complement(656..922)
                     /locus_tag="JD016_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HAU5001561.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            complement(922..1131)
                     /locus_tag="JD016_18255"
     CDS             complement(922..1131)
                     /locus_tag="JD016_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HAU5001562.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            1400..2260
                     /locus_tag="JD016_18260"
     CDS             1400..2260
                     /locus_tag="JD016_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAU5001563.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2308..>3265
                     /locus_tag="JD016_18265"
     CDS             2308..>3265
                     /locus_tag="JD016_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HAU5001564.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMF"
BASE COUNT         1255 a          376 c          535 g         1099 t
ORIGIN      
        1 acacaacagt aaattataaa ttatcaaata ataatagtta tacaaataaa aattttagaa
       61 cattttttga gattcttcta actcgtaagc atccattaag gcttcaccaa atctttgata
      121 gtgaattatt tctctttgac ctaagaattt taatacatct ataacatcaa cgtcatctgt
      181 taggtttata agttgataat aagttgcaag tgctttttgt tcagctgcca tatcctcatg
      241 taaatcggtt acgggatttg acataactgc tataggtctt acatcaaatg gaacaccatt
      301 tgaatctgtt ggataaattc catatccatt ttgagcatag tttgaaccaa gacctgcagc
      361 ttttaactct tctgggcttg catcagaagt taactgataa accatagtac atataagctc
      421 aacgtgagct agttcttctg taccaatatc tgttaaaagt gcacgcatat taccagtagg
      481 cattgtatat ctttggctta aatatcttaa agatgcagca agttctccat ttggcccacc
      541 aaattgagtt ataagaaatt tagccattct agggtcacaa gtttttatat taactgggtg
      601 ttgtatagtt ttctgatata tccacatgaa ttttcaatcc tccaatactt gatgtctaat
      661 aattaaattc tctgtcccaa ggccaaggtt ggtcagccca ttgccaaggg catctacttg
      721 gagcataccc aaagttagtt aaaggaccgt atttattttc gtaagcatat ctagcttgcg
      781 caaattgatt acataatgaa ttataagtgt tgatagcatt tttatcatca gggtgattgt
      841 ctaaatataa gttcatatca acacatgcaa aagatgtttc ctgaactttt ctcataagtt
      901 cagctctaga tgagtctttc attatcttct acctcctcta tatttttttg gcttagcata
      961 taattctttg ttgtacattt cagactctag taagtttagt tctggaaata tagttccaaa
     1021 ttttaatgca tcagaaaggc agtacatttc tgtgaaaatt tgattattaa tgtatggtct
     1081 agccaaagat ggaccacaat tttttttgat atctctagtc ttattttcca agatgaaaac
     1141 ctccttgttg taaatagata ggtaactcta tctagtgtat aatattcaat catttaaaaa
     1201 tcgttactaa taataaaata ttaaatgaga aaaataaaaa agataataaa agaaagaggt
     1261 tatttatgtt attataagtt tatgtgttat tgtaaactat aaattattta gtaaaattaa
     1321 agtaaaaatt atagtatttt gtggatgaag gtagactttt ttgtaaaata ttattaacat
     1381 aaatatacga ggtgattaga tgagtgaact atcaggaaaa ggctgcgaaa ttatagttcc
     1441 ttttgaggaa agacttccag taagagatat agaaaaaagt ataataaaaa aatatagaaa
     1501 aaatttatgg tctaagttta tgaaggcaat aagagattat aaattggttg aagaaggtga
     1561 taagatagct gtagcgattt ctggtggaaa agacagtatt cttatggcta aaatgtttca
     1621 agagttaaaa aaacatggac aagttaattt tgatgttgaa tttattgcta tggaccctgg
     1681 atatcatgca aatataagac aattacttat agataattgt gagtatctaa atataccaat
     1741 acatttattt gattcaagaa tatttgagat agcagatgaa atagcaaaag attatccatg
     1801 ctatatgtgt gcaagaatga ggagaggagc attatattca aaggcagaag aattagggtg
     1861 taacaaactt gcattaggtc atcattatga tgatgtaata gagacaacaa tgcttaactt
     1921 attgtgtgct ggaaatttta aaacaatgct tcctaaactt aattctacta attttgaagg
     1981 tataaaaatt ataagaccac tttattatat aagagaagaa catattataa gatttattca
     2041 aaatagtggt atatggccat taaattgtgc atgtatggtt gctgctaaaa aaacaggtaa
     2101 taaaagatat gagataaaag acttaataaa gagcttagaa tcaaatttta aaaatgtaga
     2161 aaaatctata tttaaggcag cagaaaatgt aaatcttgat tcagtacttg gttggcaaaa
     2221 agatggggag aaacattctt tcttagaaaa ttttgaataa aaatagttaa aaagaatcac
     2281 tataaacaat tggaaagggt gtttatattg aaaagagtat atatagattt tgaaatgaat
     2341 atgccaaatt ctaaatcaaa aagagcagta tttaattctg atataattgc aatcggagca
     2401 gttatgtatg atgaaaaaac aaaaaatatt gataaattta aatctttaat aagaccagta
     2461 agtaatgagg agttgtatcc tcatatacaa gaattaacac atatatcttc tgaagaattg
     2521 aagagtgcac caagttatga agaggtcatg agaaagttta aaaaatggct tggcatattt
     2581 tctgatatta aaggaatata tacttttggt aatttggatt tgacatgttt caataacaca
     2641 gatatgagaa gtgctaaaaa aaataaccat ccaaggtttg taaataacat tagagattta
     2701 tttgtagata taaaagaaaa atatataaat tgtggtatga gatgtatgaa ctatatatca
     2761 ttgaaaaatt tacttgaatt tgttaactta gagtttagtg gagatgccca tgacccatta
     2821 aatgatgcat ataatttatt tattttggat gaggcaatca caaataatgt agatatacaa
     2881 aatcttttaa taatacgtga cataataaga ccaccattta acgatataaa ttctaattta
     2941 gataattgtt ttaacaaatt taaggagtca ttatataaaa aagaaggaaa ttataatatt
     3001 gtagattttt ctgttgaaat aataaaaact gtgagaatgt acttattaac aatcatagat
     3061 gtaaatatac aaaatttaga aattatgaaa gatataagta aaaaaatgga tactatagat
     3121 aaattgaaag atatagagga aggctatttt tatttattag aagatgtgta cttcgatatg
     3181 aaagatgtac tagaagattt aatgctttat agaatgaatg aagatgagta caagtatgag
     3241 ataaaaaata taataaaaat gtttg
//
LOCUS       DACXMZ010000304         3264 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.305, whole genome shotgun sequence.
ACCESSION   DACXMZ010000304 DACXMZ010000000
VERSION     DACXMZ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.305"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..359)
                     /locus_tag="JD016_18270"
     CDS             complement(<1..359)
                     /locus_tag="JD016_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009109553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAU5001565.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGY"
     gene            complement(380..1459)
                     /locus_tag="JD016_18275"
     CDS             complement(380..1459)
                     /locus_tag="JD016_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HAU5001566.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            complement(1513..2802)
                     /locus_tag="JD016_18280"
     CDS             complement(1513..2802)
                     /locus_tag="JD016_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HAU5001567.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     misc_binding    complement(2965..3176)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1078 a          658 c          358 g         1170 t
ORIGIN      
        1 tcatatccac cagataccaa tattgaatct aattctattt tttctcttaa gtcttctggc
       61 caatctataa atggaagaat ggcagtagtg gctggctttc ctattcttat catagccaca
      121 ttattaagtc tttttctttc tacaatagca tttgtacaat gagttgtacc aagcatagca
      181 tattttatta atgaatggtc aatcttactt tcatctataa ctttttttaa ggcattgtaa
      241 acaccagttt caacatcttt tgtagttgcc accttaactg aatgaattac ttttaattcc
      301 ccatccagta tacaagcatc tgtatttgtt cctccaacgt caattcctac acgatacatc
      361 aaaatttcct cctaaacttt tattttcctt gtaattcttc tactgtttta tattccacat
      421 catatccaaa gtatccaggt ccaactgtct caattccttt ttctgttctc cacttttcat
      481 tacaaggaaa agctactacc ataacacgag agccatattt tattctctct gtagttattg
      541 gaaatccagt ttctaaatct aatactgcta ttaaatctgg tgtaatacaa agtggtgtat
      601 cacctttttt ggcaattaaa ttctcatttt ggaataatat agaatactct ccatcatact
      661 cattaatccc agcaaaaaag gcatgtcctc tagtaaatcc acctttcaac tctctcttaa
      721 tatcaattac ttttccttca aataacttgt atccacacaa ttcttttagt atattctcaa
      781 cagcaccttt tgaatcagat ttattttcaa gtaaaaaccc tcctatagtt tcagctattg
      841 taagagtatt acctatagca gcttttttaa cttgagctcc acttaaatta tagtcactaa
      901 tagaagaact tcctcccatt tctacagtta aagttctagc taatctttct gaccaaattc
      961 catccacagg ataaaggatg gcagaattac ctttttcatc agacattacg gcaggagatg
     1021 cctcatatcc ttcaagataa aaagttacca tttgagcttc agggaaagct ctacccatag
     1081 aatctgcatc taagatagga agtccttttt tagcggcaac tgctataggc ataagtgagt
     1141 ttatccctcc tatttctata ggaattataa catccacttt tctatttaat aatttctcca
     1201 ttatttctaa aggggtagtt aactctactt ctgaaagtaa tttttctatt gtaattacag
     1261 gagaacccat accagatact ggaactacca aagcatcatc atcaagttca tctaaactta
     1321 ttacttttac aggaccatac tttttaatgg cttgttttgc aaccaattta cctacatatg
     1381 ggtcacctcc acctccagtt ccaagcactg ctgcaccaac tgctatttta tctacacttt
     1441 cttctgttaa aaatctcatg ttactttctc ctcatatttc taatttattt tatactttat
     1501 aaacaaattt ctttatactg ctttgtctat ttcttgctta ttttttgttg aacaaagcac
     1561 ttttccaata atagattgta ctacaaaccc tgctataaaa ccatctaaag gtgctatagt
     1621 agtaagagtt ataaatccat atgttgaaag gtaagttatt aatataccaa ttatccaaga
     1681 cactattgaa cgtttaacta ttggtttata taatactcct ttatcaaagc ctttaagttc
     1741 ttgctttact atgtaatatt ctgctgtata tacaccacca acaggtgcaa ttacaattcc
     1801 aagtaaattt aaaaatccta taaaaccttc atatattcca aatacagcca ataatgtcgc
     1861 tataatacca aggactatag ttaatacttt ctcaggagcc ttcttaagta caagagctat
     1921 gcttaatgat gctgaatata cattacttgt atttgtagtc cattgtgcta atgtaagtac
     1981 tattatacct ggtatagcaa ttcctaatgt aataaaaatt ctcataatat ctgaagttcc
     2041 catacatttt actaatacta tcgaaactat tatcataaaa gcatttccaa attgtattcc
     2101 caaaaagctt gaaagagcac agtctcttct acttttagcc caacgactta tatcaggaga
     2161 tacaattgca ccaactatta ttatactcat tgacagagaa actccacctg caaatgtcat
     2221 agtagatttc atattatcat caactgaaat tccatttgct ctaaaagcta agaatatagt
     2281 tagcatcata agaactaata aaaatggtac tgaatacaca ctcaacttct ccattgcctt
     2341 atatccataa attgcagtac tcatcatcaa gagaccacct attaggctta aaatctgcat
     2401 actgacatct aaattaaata tatcttttat tattatctgt gcattttgag caaagaaacc
     2461 tacttgaact ccaaaccaac ctaatagaga aacccctata actagagaaa atatttttgc
     2521 tccagcttct ccaaatacat actttgttat catagatgta gataaacttg tagatgaacc
     2581 aaccaaagag catgtagtat acaaaattgc agaaaaaaat gaaccaacaa ttactgacaa
     2641 tattgcttgt ttcattggca tactctgtgc caactcagcg cctaaaataa cagatgataa
     2701 atctacacct attgctacta aaactgaaaa catagctacc caaccacgtt tttgattctc
     2761 tggaatctgt tccagagcat agttaatttt ttcttcttcc attttcatcc ctccatttaa
     2821 atcgtaaaac tactgacctt agcatatttt acaattttct aatttataaa tttattaact
     2881 atcatatttt tataattttc atttaatata tagttttaaa ttttctgaaa taagtttata
     2941 ataaaaaacg cctcttggaa aaatccaaga gacgaatata aatccgtgtt accactctta
     3001 ttagtaaaaa ttaattttac tcactcattt tcttaaggcg aattaaacca ctattctaac
     3061 ataattacaa tagtattctc caaagtcctt ttcacataaa ttccaatatt agttctcagc
     3121 caaatctaac tctctgtaaa attcctttat gctactttct tcttctacga atatataata
     3181 ttgatttgta gttaatttat gatttatatc ataatatata tgcaatattt tgtcaactta
     3241 aaaagtcaat tacttgcatt tttt
//
LOCUS       DACXMZ010000305         3256 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.306, whole genome shotgun sequence.
ACCESSION   DACXMZ010000305 DACXMZ010000000
VERSION     DACXMZ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.306"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..87
                     /locus_tag="JD016_18285"
     CDS             <1..87
                     /locus_tag="JD016_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAU5001568.1"
                     /translation="EYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            87..281
                     /locus_tag="JD016_18290"
     CDS             87..281
                     /locus_tag="JD016_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001569.1"
                     /translation="MEGGDYDASVAHYCLHKFKWKPHEYTDLPDYERAFVAASIDIKV
                     EEEIKEEKKNAKEARRSRRR"
     gene            351..1118
                     /locus_tag="JD016_18295"
     CDS             351..1118
                     /locus_tag="JD016_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAU5001570.1"
                     /translation="MGLFGKKKADICCICDTEIGVLNIEDGWICNSCFKEYCDALSMT
                     KAPKILKKLDIEKTISSTKKNNELQKIFNPTNNIENYIEFDEDNKKWLVSKRSENDKK
                     APIIHSYENIVKFEILEDGESIVSGGVGRAIVGGALFGATGAIVGGVTGKKTARKVVN
                     SFKIKITINDIDNPVEYIELINKKTKTNSSVYEKAYKDAHKILSTLSAITQSIKVADN
                     INSNSVADEILKYKNLLDIEAITQEEFDTKKKELLNL"
     gene            1185..>3256
                     /locus_tag="JD016_18300"
     CDS             1185..>3256
                     /locus_tag="JD016_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAU5001571.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMTNAMNIVLSSFEQLQRTSSNAID
                     ANSIRTAREELARAEAGFDRLEQQIREADGQQRRLNKDINKGASSTDKLVANAKRLVA
                     TYIGLRSVGGLINLSDQMTSTNARLNMINDGQQSDGGLNKMIFQSAERARASYLDTAK
                     IVTRIGMNAGKAFSSTKEIVAFAEQLNKKFVIAGATTEETNSALLQLTQGLGSGVLRG
                     EELNAVFESAPNIIQSIADYLDVDIGKIRSMASEGMLTADIVKNSLLAAAEQTNAEFE
                     KMPYTFGQIWTSIKNNVVMIFGVIQKKIEQSMSSRGFRTFIDNFINGLYVLGNVFFNI
                     FNGIISILSSPFFQAFANTIIVCVSLIAQALGWVITQALNIVNVFAQNWSIISPIILG
                     IASVFIIFQAVLLATKIATLGNVIANIAHAAASSFSALMTNIQAAALMRANGATLSAT
                     IATWGLNAALLANPITWVVLGFLAFIVIIFVAVAAMNKFAGTSLSAIGVVTGALAGLT
                     AFIINSVFFWVNVFISFAEFFTNVLDHPVYSVKKLFFNLATAVLNNLISMTQGCDEFA
                     TNLANSIIDGINGALKAWNKFVDVLNKFGGLGDKLGLGKADMVGHTKSITSTLQKAKG
                     DLNKWLGAEPEGYKSFKQLEYKNVGDWAKNGYAFGQNLENKIKDAFDIRKIAEKAKKD
                     LGLDDLWD"
BASE COUNT         1236 a          395 c          649 g          976 t
ORIGIN      
        1 gaatatacag acctttcaag tgaggtagga gaggtgaatg gttttgatag gacttttgag
       61 gataaagtag aagaagcaaa aaactaattg aaggaggcga ttatgatgct agtgtagctc
      121 attattgcct tcataaattc aaatggaaac cacatgaata tacagattta ccagactacg
      181 agagggcatt tgttgctgct tctatagata ttaaagtaga agaagaaata aaagaagaaa
      241 agaagaatgc taaagaagct agaaggagta gaagaagata atatctaatg taaaaatttt
      301 actaatatag tataataatc ttataaaatt attgtactgg aggaaaactt atggggttat
      361 ttgggaagaa aaaagcagat atatgctgta tttgtgatac tgagatagga gtgttgaata
      421 ttgaagatgg ttggatatgc aattcttgtt ttaaagaata ttgtgatgca ctttctatga
      481 ctaaagcacc taaaatttta aaaaaattag atattgaaaa aactatatca tcaactaaaa
      541 aaaacaatga actccaaaaa atatttaatc caactaataa tatagagaac tatatagaat
      601 ttgatgaaga taataaaaaa tggcttgtgt ctaaaagaag tgaaaacgat aaaaaagctc
      661 ctatcattca ttcttatgaa aatattgtga aatttgaaat tttagaagat ggagagtcta
      721 tagttagtgg aggagtggga agagctatag ttggaggagc tttgtttggt gctactggag
      781 ctatagttgg aggagtaaca ggcaaaaaaa cagcaagaaa ggttgttaat tcatttaaaa
      841 ttaaaataac aattaacgat attgataatc ctgttgagta tattgaattg attaataaaa
      901 agactaaaac caattccagt gtttatgaga aagcatacaa agatgcacat aaaattttat
      961 caacattatc cgctattaca caaagtatta aagttgcaga taatataaat tctaattctg
     1021 tagctgatga aatattaaaa tataaaaatc ttttggatat tgaagccatc acacaagaag
     1081 aatttgatac taagaaaaaa gaattgctaa atttataata tataagcact tacttaaaaa
     1141 taagtaagtg cttatattat gttaaaaaag aaaggaggtt aaaaatggca actatacaaa
     1201 catctatccg aattttcgac ggaatgacac ctgcttttag acacatgact aatgctatga
     1261 atattgtatt aagttcattc gagcaattac aaagaacatc tagcaatgct atagatgcta
     1321 atagtattag aacagctaga gaagaactag cacgtgcaga agctgggttt gatagattag
     1381 aacaacaaat aagagaagct gatgggcaac agcgaagact taataaggat ataaataagg
     1441 gtgcaagtag tacagataaa ttagtagcaa atgctaaaag attagtagcg acttatattg
     1501 gattaagaag cgttggcggt ttaattaatt taagcgacca aatgacgagt actaatgccc
     1561 gtttaaacat gataaatgat ggtcaacaat cagatggcgg acttaataaa atgatatttc
     1621 aatcagcaga aagagcaagg gcatcttatt tggatactgc taaaatagta acacgtatag
     1681 ggatgaacgc aggtaaagcg tttagcagca caaaagaaat agtagcattt gctgaacaac
     1741 ttaacaagaa atttgtaatc gcaggagcaa caacagaaga aacaaattca gcattactgc
     1801 aattaacgca aggattggga agtggtgtac ttagaggaga ggaattaaac gctgtatttg
     1861 agtcagcacc taatataata caaagtattg ccgattatct tgacgtggat ataggaaaga
     1921 ttagaagcat ggcaagtgag ggaatgttaa cagcagatat tgtaaaaaac tcattacttg
     1981 cagcggcaga gcagaccaat gcagagtttg agaagatgcc ttatacattt ggacagattt
     2041 ggacatcaat aaaaaataac gtagtcatga tttttggtgt tatacagaaa aaaatagaac
     2101 aatctatgtc tagccgaggt ttccgaacct ttatagataa ttttataaat ggattatatg
     2161 ttttaggtaa tgtgtttttt aacattttta atggaattat aagtatatta agtagtcctt
     2221 tttttcaagc tttcgcaaac actattattg tatgtgttag cttgatagcg caagcattag
     2281 gttgggtgat aacacaagcg ttgaatattg ttaatgtatt tgcacagaat tggagtatta
     2341 tttctcctat aattttgggg atagcttctg tttttataat atttcaagca gttttactag
     2401 caactaaaat agctacctta ggcaatgtga ttgcaaatat tgctcatgct gcagcttctt
     2461 cattttctgc actaatgact aatattcaag cagcagcatt aatgcgtgca aatggagcga
     2521 ctttatcagc aacaatagct acttggggat tgaatgctgc acttttagct aatccaatta
     2581 catgggtagt attaggattt ttagcattca tagtcattat atttgtagca gtagctgcaa
     2641 tgaataagtt cgcgggaaca agtttatctg ctataggtgt tgttacagga gcattagcag
     2701 gtttaacagc atttattata aatagtgtgt ttttttgggt taatgttttt atatcgtttg
     2761 ctgagttttt tacaaatgta ttggaccatc cagtttattc agtgaagaaa ttatttttta
     2821 atttagctac agcagtatta aataatctta tatctatgac acaaggatgt gatgaatttg
     2881 ctactaattt agcaaactct atcatagatg gaattaatgg ggcattaaaa gcttggaata
     2941 aatttgtgga tgtcttaaat aaatttggag gacttggaga taagctagga ctcggaaaag
     3001 ctgatatggt tggtcataca aagtctatta caagtacact acaaaaagct aaaggtgatt
     3061 taaataagtg gcttggagca gagccagagg gatacaaatc ctttaaacaa ctagagtaca
     3121 aaaatgtcgg agattgggct aaaaatggat atgcctttgg tcaaaactta gaaaataaaa
     3181 taaaggatgc ttttgacatt agaaagatag ccgaaaaagc aaaaaaagat ttaggcctag
     3241 atgacctttg ggatga
//
LOCUS       DACXMZ010000306         3232 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.307, whole genome shotgun sequence.
ACCESSION   DACXMZ010000306 DACXMZ010000000
VERSION     DACXMZ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.307"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            171..1538
                     /locus_tag="JD016_18305"
     CDS             171..1538
                     /locus_tag="JD016_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAU5001572.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            1563..2171
                     /locus_tag="JD016_18310"
     CDS             1563..2171
                     /locus_tag="JD016_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HAU5001573.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            2219..2590
                     /locus_tag="JD016_18315"
     CDS             2219..2590
                     /locus_tag="JD016_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAU5001574.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            2769..3107
                     /locus_tag="JD016_18320"
     CDS             2769..3107
                     /locus_tag="JD016_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAU5001575.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
BASE COUNT         1383 a          237 c          569 g         1043 t
ORIGIN      
        1 attaaaatta aaagtaaaaa actaaataaa ttaatgttaa tttgaaatat atatgaaggt
       61 ttttggagaa tacttactta tgataggaaa aaatgttaaa ataatagtgt aatcaaatat
      121 aaaggactga aataattaac agttattaaa tattaggagt gtaaaaattt atgggattaa
      181 aagaatttga ttttattgaa gagtctattg atatgttgag gacaatgtca ccaacattag
      241 aaactatatc agatgaaata gaagagtatt ttgaaaatat cttggatgaa aagaatcaag
      301 aatatataaa tgtaacctct agaataaaat cagaatcaag tttaagagaa aagataatta
      361 gaaatagata tttaaaaaaa tatggagaag caagtaatct tattcataat gtttctgact
      421 taataggcct aagaattgag tgtagattta ttgaggatga aaacaaaata tatagacttt
      481 tgagaagata ttttaataaa acagatgaca aaataaacta ttataataaa gagaataaga
      541 atattaaatt aaaactatct gagagacaac ccaataagca aaaaaatgga tttgaaatat
      601 ataaaataga tggagttttt gcgtatttag atagagaagt aaaatttgaa cttcaaatta
      661 aatctcttgt aaatgtgttt tggagtgaaa tagaacataa aataatttat aaaaacaata
      721 catatttact tgctgataaa tttataaagg atatgatgga ctctataaag aataatctta
      781 ctatgataga taatcagctt ttaagtattt ataaaaactt tcattcaggg aagtctttta
      841 atatgaaagt aagtaaaaaa gaaattgaaa aattatttgc gaaattagtc tatgacgctt
      901 tttctgaaaa gatgaataaa agtataggtt ttgttgtaga ttttaaaaaa ccatgtgaga
      961 ctatattgaa ctattctttt aataaacaag atataagtga tgaagtttta ggtaatttta
     1021 tgcttgatga gtttgcaaga ttaaatgaaa ttgtaaacaa ggatatagat tttaatgagc
     1081 aaatagaatt tgaaagagaa ccaacatttg atgataagtt ttgtaaagac ctaggaaatc
     1141 attttagaag tagattaaac acagaatttc catggaacct attttttaga atattatttg
     1201 aaatagagcc atataacaat accaaagact ttgaaaactt tgtagagttt ataaaagaaa
     1261 atgtgctact agaagaaaat agggagcaat tattatgcca atttgaagaa gatagcaaac
     1321 ttataattga agatatatat gattgtatat ttaatagtat aagtgagatt gatagtgttg
     1381 aaatcttata taactataat cttgaaaaaa taaactttac atcaagtgaa atcataaatt
     1441 gtatatgtag agaatatgag tgttatggtg aatacttaga agaaaaagaa aagcttatga
     1501 atacatttaa agaaaagatt attcaaatct ttgagtaata ggttaatgct gaagtggcgc
     1561 taatgcgcca tttttcaaat atatatgaat taagtaaaat tattttggag gaggcgattt
     1621 gtatagagaa aacaggtgta attttggcag ggggaagaaa ttctagaatg ggtagagata
     1681 aagccttttt agagttacat gataagcttt ttattgaaat tgcaatagaa gcatttaaaa
     1741 actttgatga gttgataata atatctaaca atgaagaatt atattctaaa tacgatatta
     1801 aagtttataa tgatatagta aaagatgttg gtccaattgg aggaatatat actgctcttg
     1861 aatatgcaaa gtatgatata gttacaattg catgtgatat gccatatcta aataatcaaa
     1921 tagtcgaaag aatagctaat aaaatggatg ataaaagtgt gatttcagtg acaaatggaa
     1981 agttacaacc attgtgttct ggatataaaa agagtataat aaataaagta tctttatgta
     2041 taagggaaaa tgatttaaag ctcagaagct ttatagataa aatagataaa agttatatat
     2101 actttgatga agaagattta tttttaaatg taaacactgt tgatgagtat gaaaaattaa
     2161 ctaaagttta attagctatg gttgtaaaat agagtaggat ttatatggag gagagtttat
     2221 gaatttactt aaatgcgatt cttgggcagt aatattaaat aatagcgata aagaaagtaa
     2281 agcatataaa atattagatg aacttaaaag gaatatgtac aaagttgttg ctatagatga
     2341 agagaaaaaa cctattgaag gaatagatgt gtatgaatgt ttaaaagatg ttccacacaa
     2401 tattgatgtt gtagctatta ttgataaaca atctaagatg gatgtgattt tagaagaagt
     2461 agagcttttg gatatacaaa atatgtggtt tgaaaaagga agctttagtg aaatgatgat
     2521 taaaaagact aaagatttaa aattgaatat agaatataat ttaagtttat atgatgagtt
     2581 gactagataa aattatattt cttaataata aaaatataat tagttaataa aacatattat
     2641 tttatagtat agattggtta ataaaagtac agattggtta atgaaacatt aaattacaat
     2701 atttgttaaa aaaattgtga gttacatgat ataatgtatt aaataggata ctaaggagtt
     2761 gatatattat gttaatagta actacagaaa aagtagaagg taaaaagata tcaaaggttt
     2821 taggattagt gagaggaagt acaataagag caaaacatgt tggaaaagat ataggagcaa
     2881 gttttaaaaa tcttgttgga ggagaactta ctggatataa tgaaatgctc actgaagcaa
     2941 gacaaattgc cataggtaga atggttgaag atgcagaagc taaaggtgca aacgcagtaa
     3001 tagcatttag actgtcttca gcttcagtta tgcaaggggc agcagaaatg cttgcttatg
     3061 gaacagcagt tgttttagaa gatgataata gtattcttga aaaataaaat ataaatagtt
     3121 aaaaagaggc atcttttaaa aaattttatg gatgcctttt tttatttgaa aaaactaatt
     3181 tgagaagaaa ttttaactat tatgacaata aaattagggt gatattatat aa
//
LOCUS       DACXMZ010000307         3212 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.308, whole genome shotgun sequence.
ACCESSION   DACXMZ010000307 DACXMZ010000000
VERSION     DACXMZ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.308"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            28..639
                     /locus_tag="JD016_18325"
     CDS             28..639
                     /locus_tag="JD016_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAU5001576.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            937..1275
                     /locus_tag="JD016_18330"
     CDS             937..1275
                     /locus_tag="JD016_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HAU5001577.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(1299..1435)
                     /locus_tag="JD016_18335"
                     /pseudo
     CDS             complement(1299..1435)
                     /locus_tag="JD016_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424152.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
     gene            complement(1681..3117)
                     /gene="miaB"
                     /locus_tag="JD016_18340"
     CDS             complement(1681..3117)
                     /gene="miaB"
                     /locus_tag="JD016_18340"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAU5001578.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT         1099 a          473 c          374 g         1266 t
ORIGIN      
        1 ttaataatat tattttagga ggtaaaaatg ataaacgata ttaaaaataa attcaaaaat
       61 tacaaaccat atataaatgg atacaagaat atgaagagag catctgtttt aattccaata
      121 gtagaaataa ataacacaca ctatatatta tttgaagttc gctcaaaaaa tttgaagcat
      181 caacctagcg aaatttcttt tccaggtggt aaaatagaat caggtgagac tccatatgaa
      241 gctgttatta gagaaacttg tgaggaactt ggaactttta gtgacaatat agaagtaata
      301 tcaagtcttg acctccttat aacaccagta aagtttatca tacatccata cttaggatat
      361 ataaaaaata ttaataattt aaatataaac aaggatgaag ttgaccatac ttttttggtt
      421 cctataaaat atcttttaga gaatcctcca aatacttata gtaatactgt aaaagttttt
      481 ccagatgaaa atctaccata tggttcaatt ccaaaccaaa aagattataa atttgaaagt
      541 ggaagttatc ctattctgtt ttataaatat aaagattatg tgatttgggg aatgacagca
      601 aaaatactag aaaacttttt aaatttttta tcagaataac actcataata aattatcaaa
      661 acttttacat aagaaattta taaaagtttc taaatttaac aattctaaaa tattaataac
      721 tatattttgt agcacttttt tattttatta ttgtttgaat atttgatggt tttcaatgca
      781 atttcaacat aaatactatg tattagtgta tactgttgat gaattaaagt atgataatct
      841 atttaataaa gtatgaagta aatgatttta atgtgccttt atggtaaaat tactaataag
      901 taataaaatt aattaaaata tatggaggtg cttataatgt ttgctgtagg atttgttgtt
      961 ataattgcac ttgccttttt cattttaagt gtactattat ttaatggaaa agccagtatg
     1021 ctactagcag gttataactc tatgagtaag aaagaaaaag cacgatacga taaaaaaaaa
     1081 ttatgtagaa gttctagcat tgttactttt attgtctcta taatgctatt tattatggcg
     1141 tttttgggat atagaataga gagtggtcaa atggacgaaa aaaaaatggt tcccttttct
     1201 ataatattta taatagtcat actttccagt gtatttttta atatcctcta ctctaacaaa
     1261 aaatgtaaaa aataaatagt tgtaagttga gcagttaatt ataaaagatt gactgctttt
     1321 tcatgtatat tgactactct tattaaatat tttaacattt aaaatctatg taaaaataat
     1381 gaatatagta caaacttcta ataatgattt tacctttgtg gttacagtcc actatatata
     1441 atttaatatg ataacaggga attactaatg acaactaggt aagcttttat aaaatataat
     1501 aaaacaaaaa tagatgtaca atgaaattat atcttatatc aaatttattt ttattaatag
     1561 caaaaactta cattagcata acatatgaca aattcgctta aaataaaaat ttttaatata
     1621 atttgttcta tagtacatct attttgatta gtttacataa ttttattatt ttgtacaata
     1681 ttaattattt actaatatcc catttaatga aaaagttcta ggttcagtaa ttttgaccat
     1741 aactagctta ccaattaaat catcatttcc accttcgaaa tttacgagtt tattttgtct
     1801 agttctacca gcaaacttat tttcattatt tttacttctt ccttcaacta gaacttcaac
     1861 tattctatct ttatatccat catttatttc tgcagaaatc ctatttacag cttctaacac
     1921 cctattaaat ctatcatgct ttatatcttc tggtatttga ttttccattt tagcagcagg
     1981 agttccctgt cttttagaat aaataaatgt aaaagctgaa tcatatctaa ctttttcaac
     2041 cacatctaaa gtgtccaata aatcttcttc tgtttctcct gggaaaccaa tcattaagtc
     2101 tgtagaaaaa gcaatacctg gaacttcctt tttagctttt tctattattt ctaaataata
     2161 ttcctttgta taatgtctat tcattttttt caaaaggcta ctgcttccac attgtattgg
     2221 tagatgtaaa aactcgcaca ccttatcaca atctctaatt gcgtatatta cttcatcaga
     2281 tatatctttt ggatgagatg tcataaatcg aaccctttca attccttcta tatcattcac
     2341 ttttcttaaa agttctgaga atgaaactgg attttcaagt gtcttaccat aagaatctac
     2401 attttgtcca agtaaagtga tttctttcgt tccatttgca acaagctctt ttatctcgtt
     2461 aattatatct tcaggagttc tacttctttc tcttcctctt gtgtatggga ctatacaata
     2521 tgtgcagaaa ttattgcatc catacattat atttacaaat gcttttaatt caaattttct
     2581 attacttcta agaccttcta ttacttctcc atccacatcc catacatcca ctaacatttt
     2641 gtcagaattt attgattctg taagtaattg cggaaattta tatagattat gtgttccaaa
     2701 tataaggtct acatgtttat atttttttct taattcctct acaacatgtg gttgttgcat
     2761 catacatcca catacagcta ttttcatatc tggatttttt ccttttagat gttttaattg
     2821 tcctaagtta ccatacactt ttagctctgc attttctcta actgcacagg tgttatatat
     2881 tattaaatca ctttcttcaa ccatcattga catttggtat cccatctctt ctaacattga
     2941 acataatttt tctgaatcat gttcattcat ttgacacccg aaggtttgag taaacacgag
     3001 ttttctcttt ccagttctgt tgaagtattc ttcattttgt ctgtgtattt gttctatata
     3061 tttatcttgt tcttgtattt tctctatagg tattgttact tgttttcttt tgctcacgtc
     3121 tattcctcct agttaatttc ttatattaca cataacctta attatatcaa ataagaaaaa
     3181 tcttttaaag atattattat ttttctagtt gt
//
LOCUS       DACXMZ010000308         3195 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.309, whole genome shotgun sequence.
ACCESSION   DACXMZ010000308 DACXMZ010000000
VERSION     DACXMZ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.309"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            151..1230
                     /locus_tag="JD016_18345"
     CDS             151..1230
                     /locus_tag="JD016_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAU5001579.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVEILRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATRLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            1343..1942
                     /locus_tag="JD016_18350"
     CDS             1343..1942
                     /locus_tag="JD016_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001580.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(2129..3001)
                     /locus_tag="JD016_18355"
     CDS             complement(2129..3001)
                     /locus_tag="JD016_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAU5001581.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
BASE COUNT         1231 a          332 c          520 g         1112 t
ORIGIN      
        1 ctaactaaat aaaaactaat gaagtattaa aaaaaatgaa attaataaaa tatgaaaaaa
       61 ttttaggaat aaagtatctg ttagataaaa ataagattaa ttagtgatat aatataatta
      121 agataaaatt tattatttgg aggtaaaatc atgagcagag taaaaaatgt agtagaattg
      181 ttggaaacta aaggtgttga tgcactatat ctaacaaaga aaactaatgt aaactatata
      241 agtggttttc ctgatgaaga agcatatgca gtgatttgca aagatggaaa ctttttagtt
      301 actgatagta gatatatgga gcttgcagaa aaggtttgca aagattttga aattataaat
      361 tggcataatt ttgatagaag tgtggcaaag gcagttaaaa gtgtatgtga taaagtgggt
      421 ataaagaaac ttgggtttga aagaactaat atagtgtttg ataaatatga agaacttaaa
      481 aatctgattg agaaggataa tggagaatta atacctacag aaaatattgt agagattttg
      541 agatatgtaa aagacaaaga tgaaatcaaa aatacaagaa aagcttgtga aatagcggat
      601 aaggcattag aagaattgat tccacatata aaagctggag ttagcgaaat tgaactggct
      661 acaaggttag agtactttat gaaaatgaac ggagcacaaa atatagggtt tgaaactata
      721 ctgatatcag gagcaaagac ttcattgctt catggtaagc ctagtgataa gattatagaa
      781 aaaggtgatt ttgtacttat agactatggt gctatgtata atggctatat atctgataca
      841 actagaactt ttatagttgg aggagcttct gaaaagcaat tagaaatata taatttagtt
      901 aaggaagctc aaaatgtagg ggttgagaac atgaaagctg gtgtacatgc gacaattcca
      961 gatgctgaga taagaaaagt tgtcaaaaaa tatgaagact attactatca aggtatagga
     1021 catggagtcg gtagagatgt tcatgaagaa cctttcattg gtaattatgg tgataaaatc
     1081 atagaagagg gatgtataat aacaatggaa ccaggtattt atttccctgg atggggtgga
     1141 gttagaatag aagatactgt tttgattact aaaaatggtc cagaaagact tactaaattc
     1201 cctaaagatt tgatgatttt agacaaataa tttaatttat atatattttt ttacataaaa
     1261 tataacataa tctcactatt aagctctatt tacatattat agtaaagagg gcttaatttt
     1321 attgtgagag gtgatagtat atatgtataa aaaacataaa tttataaaaa atataaaaaa
     1381 agcctggtat aaaatcaaat taattgatag atgccttata attgtaatgg caatattaat
     1441 gtttcaatct atatataatt tatttgttaa tgaagtaaat tcacaagata ctacaactat
     1501 tgatgtagta gtaagaacca cttcagcagc tatatttggg tattttttaa gtgctaattt
     1561 tataaagaga cctttaagga agtatacaaa tactgatgta tcttcaaatc tttttataga
     1621 taataattta tccccaaaag gtcaagagtc tagtcaaaat aatataatga atgtcaaaaa
     1681 taccattgga tttacttcag aagctgataa ttatgaaaag aaaataccta ttaataataa
     1741 tgaaggtttt gaagaaggag aaactagtga attgcaaatt attatagcta ctgtaatttg
     1801 tatagtagca ttaacagtac tttttgttgt aagaaatttt acaactacaa catcagcttc
     1861 tttaggtact atttcacaga tgagagattt tgtctcagga tgcgtgggtt ttttgctagg
     1921 ttgtccaagt aaacctgatt aaaaaagtat tatcaataaa aggtatttta aaaagtatca
     1981 gtataataat atttagattt aaatgtatac aattataatt ctgtaacatt ctatagtgta
     2041 tttttatact gatactctaa actatactat ttattaaaat attttaacat tgtatttaaa
     2101 caaaatacta tatctgtata atataaattt agtttatatg aatagatgcc atatactcaa
     2161 taaaaagttt catactatga gatatgtatt gatttcttct ccaaagaacc ccaatatttg
     2221 cagtaattgc acgttctaat ggtattgaac agagattttc tgtattttga aatatttctt
     2281 tatacaatat tgttgaagcc aagtcatttt ctagtaaata acgtatggta gaaagttgat
     2341 ttgagtgtaa aattatattt ggcttagctt tttcaagtct ttttgtaata atcttatagt
     2401 gataagaacc ttcagataag attacaaaag gtacattttt taacatataa ttcgatataa
     2461 tgggatgttt tgccaattca tttttgggat ttgtaactaa gtataattct gttttataga
     2521 ttgtctttga atctatatca tcataaatat cactgtcaat tataccaata caaaaatcaa
     2581 gtgtagattc ttcaatcatt ttagctccaa caaatgttgg aacttcaaaa attttaagct
     2641 ctatatctgg ataaattaca ttaaatctag gaagtatttt tgaaagcaaa aacgtaccta
     2701 aaactgtagg aatacctatt ttgagagaag cttttctctt ttttcctaaa tcaagtgcgt
     2761 cacattgaaa atcctcaaat tgttttataa aatgttttga cttgtctaaa aaaatttttc
     2821 cttcaggcgt taactctatt ttattaccta ttctgtaaaa aagttcaaag ccaagttctt
     2881 tttctaattc tttaattgct aatgttatgg atggttgaga aatatgaata ctttccgcag
     2941 ccttagtcat gtttttatat ttacagacag tttgaaaata gtataaatag tttatattca
     3001 tttggtatta gttcctttca tttacatatt agtgacaagt atataattaa caatagcata
     3061 agttttgtat attgttgtaa aaaaatagaa tagttttaaa ctatcatagc ataagttttt
     3121 gatattttca atctattaag aatagtgcta ttattaaatt aacaaaagag aatagctggt
     3181 atggtttaga gtaaa
//
LOCUS       DACXMZ010000309         3194 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.310, whole genome shotgun sequence.
ACCESSION   DACXMZ010000309 DACXMZ010000000
VERSION     DACXMZ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.310"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     misc_binding    94..304
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            530..1345
                     /locus_tag="JD016_18360"
     CDS             530..1345
                     /locus_tag="JD016_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAU5001582.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            1373..2050
                     /locus_tag="JD016_18365"
     CDS             1373..2050
                     /locus_tag="JD016_18365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAU5001583.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            2109..2831
                     /locus_tag="JD016_18370"
     CDS             2109..2831
                     /locus_tag="JD016_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAU5001584.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         1270 a          328 c          602 g          994 t
ORIGIN      
        1 tagataattt acaagtaaaa attattaaaa aaataaaaaa cttattgaca aaaatattat
       61 aaggaagtac aattgtatta acaatttaag gaaacaaatc caatgataga gaaagtagca
      121 tatatggatt tacagcgagt taggggttgg tgtaagccta gcaatgaagt ctatgtgaag
      181 agagctctgg aggagattat cagaaacttt agtatgataa ttggtttacc cgccttaagg
      241 gattgagagg ataagtactt acttatcaaa aagagtggta acgcggatat aattcgtctc
      301 ttagctgtaa agctaaggga cttttttgat tttaaaataa aaatatccat acagcttaaa
      361 agctaacagt cacaaatttt gaaagtaata taagtattta tcaataagag tggtaacgcg
      421 gtaaagtcgt ctcttagtca ttaaagactg agagactttt tttgtaactc gaaacattta
      481 agtattaaaa tctgagggaa attataatta aaagagaggg gtagttaaaa tgaaagtatt
      541 taaaaaatta ttaagtttag gattagtatt aggattgaca ttatcgctag taggatgtag
      601 tggtggagga gaaaaaacaa agttagaaca aataaaagac aatggaaaat tagttgttgg
      661 tactagtgca gaatttcctc catttgaatt tcataaggta gtagatggaa aagattcaat
      721 aaaaggattc gatataatgt tggctgaaga atttgcaaaa gagcttggtg taaaggttga
      781 aattaaggat atgtcatttg atggattaat aggtgcttta aatgcagacc aagttgatat
      841 tgttcttgca ggtatgtctc caacaccaga gagagaaaag agtgttgatt tttcagaatt
      901 atattattta agtagaaatg cagttatagt taaagatgca gatatagaca aagtaaaaac
      961 tgaagatgat ttaaagaaac ttagagttgg agttcaagca ggaagtatcc aagaagaata
     1021 tgtagttaac actttaaaaa tgacaactac aaaatctttg aaagcaatac ctgatttaat
     1081 tacagaatta aaaaatggaa acatagatgc agttgttact aatgaagcag tttctttaat
     1141 aaatgttaaa aaatatgatg gaataaaaat ggcaaatact gaagttggga aagatgtaac
     1201 agaaggtatg gcagcagcaa tcaaaaaatc tgacaataac aaagatttta tagaattatt
     1261 aaacaaaaag ataaaagaat tacaagatgg caagaagata gaagaatttt taaatgaagc
     1321 atctactgaa gctgcttcaa attagtaaga aagttatgtg ggagatgaaa ttatgagttt
     1381 agattttagt tttttaagta gatttgggac ttcttttttg gaaggaacag gtgtgacagt
     1441 atcaatttct cttgtggcat tatgctttgg atttataata ggtataatta tctgtatggc
     1501 aaaaatatca aagagcaaag tattaagagc aatatcatca atctatattg aggttttaag
     1561 agggacacca ttattagtac agatatatat agtatggttt ggtttacctc aattaggaat
     1621 aagattccct atgttgtttg gtataccaag tgaatttata gctagtgcat ttgcattatc
     1681 agtaaactct ggtgcatacg tggcagagat acttagatca ggtatacaat cagttgataa
     1741 tggacaaatg gaagcaagta gatcattagg attaaattat tggtcaacta tgagatatat
     1801 aataatacct caagctataa aaaatatatt accttcatta gcaaatgagt ttattacact
     1861 tgtaaaagaa tcttcaataa tatctgtaat aggagttgtt gagataatgc gtactgctga
     1921 tatagtaaaa aatgcagcat ttagagcatt agagccatta atagtagcag cagcaattta
     1981 ctttgttata acatttactc tatcaagatt agttggatta ttagaaaaga aactatctgt
     2041 gtctaactag atgagagcat gtttagcatt tagggaaaac atagaatagt aaaaaggaaa
     2101 gtgataatat gattacaata aaaaatttaa gtaaatcttt tggagattta aatgtattaa
     2161 aaaatataga cttagaaatc gctaaaggcg aaataatggt tatagtaggt ccaagtggtt
     2221 ctggtaaaag tacacttctt agatgtatga atttactaga aatacctaca ggtggagaaa
     2281 taatatttga aggaaaaaat ttggtcgata aaaaaactaa tattgatgag gttagacaaa
     2341 atataggtat ggtgtttcag aactttaatt tattcccaca taagactatt ttagataata
     2401 taacattagc accaattaaa ttaaaaaaga tgactaagga agaagcagaa aaaaaagcag
     2461 aaattttgtt gagtagagtt ggtcttttag ataaaaaaga ttcatatcca tcacaattat
     2521 ctggaggaca aaagcaaaga atagcaatag ctagagcact tgctatggaa ccagatatga
     2581 tgttgtttga tgagccaaca tctgctcttg acccagaaat ggtaaacgaa gttttggatg
     2641 ttataaaaga acttgctaaa gaaggtatga caatggcaat tgtaactcat gagatgggat
     2701 ttgctaagga agttgcagat agagttattt ttatagatgg aggaagtata cttgaagata
     2761 atactccaga agaagttttc ggaaatccaa aacatgaaag aacaaaagca tttttagcaa
     2821 aagtgctata atatataaaa aggatgttat tgatattaga aattcaatac atcctttttt
     2881 agtgatggtt aatattaatt ttaaaattaa agttatatta atattaactt tatttttacg
     2941 attttttgca gaaaaaaaaa gagctcgggg taagctcttt ttttccaccg aattaatttc
     3001 cgttactaat ccgattttta ataaatataa aatatatcct tatgaaaggg tattgggaat
     3061 agatatatta actaactagg gttaccaggg ggataacctt aatttattat aacaaaatgc
     3121 gacacaaaaa tcaatatttt ttttgaaata aattataaaa gttattttta taacaagtaa
     3181 gcttataaat ttaa
//
LOCUS       DACXMZ010000310         3114 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.311, whole genome shotgun sequence.
ACCESSION   DACXMZ010000310 DACXMZ010000000
VERSION     DACXMZ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.311"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            81..623
                     /locus_tag="JD016_18375"
     CDS             81..623
                     /locus_tag="JD016_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAU5001585.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            637..>3114
                     /locus_tag="JD016_18380"
     CDS             637..>3114
                     /locus_tag="JD016_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAU5001586.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKE"
BASE COUNT         1265 a          400 c          491 g          958 t
ORIGIN      
        1 tagcttacat attgattaat aaattttaaa ggagtataat aaacagtgtt cataataatt
       61 ttaataaaag aggtggtaat atgccaaaga ttttagaaaa tgtaaaagaa gatatattaa
      121 aagtgtctcg ggatatgatt ttagaagaag attactcgaa tattagcata aggaaaatag
      181 ctcaaaggtg tggtataagt gcagggacag tgtataatta ctttaattca aaacaagaaa
      241 taatagaata tatcacaaaa tctgaatggg atttattgat aaggcgaatt gaatactcca
      301 ataaaaacac tgaagattac ataaaaaaac taaacattat ttttactgaa ataagaaatt
      361 ttataaacaa agttcataat atccaataca atgacttttt aaacactttt gaaacagaga
      421 gattttttga aatgaaaaaa cacaaagacg actttcatga acagctttca aataaagtat
      481 atgaagcttt agaaaaagaa agttttttta ataatgacaa attagtatgc aatatcataa
      541 taaaaatgtt tttttcatat tcaacatatc ttcatataga atttgaagat ttaaaaccct
      601 atattgaaaa attaatacat taaggtggtg tttaccttga atcataatac tagtattaag
      661 aacaaggaca gtaatccaaa aatattttta gtacttacac tttatctatt aggtatattt
      721 atgggagcta ttgatactgg aatagtatca ccagcaagaa cgattataca aaatagcctt
      781 ggagtaaatg aaaaaacggg aatttggatg attaccatat acacattaac atacgcttct
      841 gtaattccta tctctggaaa attagcagat aaaataggaa gaaaatatgt gtatctagtt
      901 agtatatttc tatttggtag tggttcatta atatgtggat tatcatcact attttccaat
      961 ttctatattt tattaattgg tcgtgttatt caagcaattg gtggtggtgg aatcatgcca
     1021 attgctacag cagaatttgg aactaccttc ccagaaaaca aaagaggcat ggctttaggt
     1081 ttagttgggg caacatatgg tattgccaat attttaggtt caagcattgg tagcacaatt
     1141 ctaagtatct ttggaactca aaattggaag tggctatttt ttgtaaatct tccaatttgt
     1201 ttaatcatac ttattggtgg agttttctgt attaaaaaca ataagtctga atctaaagaa
     1261 aaaatagata agcttggtac attgatgttg atatgtataa tcgtatcact attatatggt
     1321 cttatgaata tagacttttt taattttaaa aattctatac aagatatatc agtttatcca
     1381 tacttattga catttattgt acttatacct attttcatat ttattgaaaa tagagctaaa
     1441 gaccctattt taagctttga atatttttta aatccgagaa tattaatagt acttatttta
     1501 tctttaattg ttggtattgg aatgatgggt atggtattcg ttccacagta tgctgaaaat
     1561 gcattaaaga taaatgctgg ttctggggga tattttgtag ctatattggg attatttgca
     1621 ggtattgctg ctcctttaag cggaagactt attgacaaat ttggggcaaa aaaaatacta
     1681 ttactaggat tttctgtatc tatgataggc tctctttacc tagttttaat tgctctaaaa
     1741 accaatactg tatttagtgt atgtgtaagt ttaatattta tgggattagg aatgggattt
     1801 actatgggaa ctcctctaaa ctatatgatg ttatcaaata caagattaga agaatctaat
     1861 tcagcacttg caacattatc cttgatacgc tcaattggta cttcaatttc tccagctata
     1921 atgattggat ttatagcaca tgcaggatta tctgtgcaag ataatattat ggacttagtt
     1981 ggaaagccct caactcctaa aatagttcaa cttgaagaac ttgaaaacat gatagatgac
     2041 ttaaaatcag accctgaaat ggcaaaaaat ttaaaaaatg tctctatccc aaatatgaat
     2101 gatacttcaa atataaatat ggacatgaca ggtggcaaac ttccaaaatc cttacttaat
     2161 aaagttcagt cagcagatac aaccaacatt accaatataa caaaagaaat atcaacaaca
     2221 atgtttgatg aaaaagttcc atctataatt actaaaatac agaaaaatgt acaaaaagga
     2281 atagatggaa cacaaagtgg aataaacggt attgaagatg gtgaaaataa actaaatagt
     2341 ggtattaaag gagttcaaac tggtattgac aatatggtaa aagctagagc tggtattaat
     2401 caaggaatag atggtgtaaa aaaaggtatt gctggtgttg ataaaggtat aaaaggtatg
     2461 gaacaaggtc ttgaaaaaca agatgaggcc atcaaggaac ttacaactgc ttataataaa
     2521 atacctacta cagaaaaaac tcctactgat aacgaaaata actcaaatga acaaaaccag
     2581 agtaatacca gtaattctga atcagacgac actaagaatg attctacaga aaataagaat
     2641 aacaattcca gcgagaataa accaaatgta aatgaacaaa aagaaagttt aaatgctcaa
     2701 atacagcaac taaaaaagac aagagatgac ttaaatacaa agttacaaaa aaataaaact
     2761 caaaagaacg aattaagtaa aaaactaaga tcaatggaat ctcaaaagaa agagctacaa
     2821 agcaagttaa ataattctat aagccaaaag aaagacatgg aaaaaagcct tgatacaatg
     2881 caacagcaaa aaaaatcact ccaagcagtt ttagcaaaga cacaagaggt aaaaactgaa
     2941 ataccaaagg cttttgataa atcaaaattg gattatataa attccataga aaataataga
     3001 acaaaaatag aaaacacact acaatctact ctaaactcag gatttaaaca gatgtatata
     3061 acagtattct gtgtaaatct actagcattt ataatattat tgttctataa agaa
//
LOCUS       DACXMZ010000311         3106 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.312, whole genome shotgun sequence.
ACCESSION   DACXMZ010000311 DACXMZ010000000
VERSION     DACXMZ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.312"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1068
                     /locus_tag="JD016_18385"
     CDS             <1..1068
                     /locus_tag="JD016_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HAU5001587.1"
                     /translation="DLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMG
                     ALSSGSTSVPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVL
                     YEGGSIVISSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQ
                     LLFEDTARNLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIR
                     EGKIFVNTEYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKIENE
                     VKKIPQIEDAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIF
                     LNSFPYTSSEKIDRLALLKML"
     gene            1144..2019
                     /gene="dpsA"
                     /locus_tag="JD016_18390"
     CDS             1144..2019
                     /gene="dpsA"
                     /locus_tag="JD016_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HAU5001588.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            2022..2612
                     /locus_tag="JD016_18395"
     CDS             2022..2612
                     /locus_tag="JD016_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HAU5001589.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(2819..3019)
                     /locus_tag="JD016_18400"
     CDS             complement(2819..3019)
                     /locus_tag="JD016_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001590.1"
                     /translation="MKKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
BASE COUNT         1194 a          387 c          479 g         1046 t
ORIGIN      
        1 gatttatatg atgataacaa taatgaagta gcaaaaaaag ttaatttttc tatttttaat
       61 cctaaattta atatatatat gtatcaatat aagaagctta taaattattt agataaaagt
      121 atttgtatgg gagctttatc ttcaggttct acaagtgttc caaaggtatt atatagaaca
      181 tatgagagtt gggctggatt ttttcctata caaaatggta tttttaatat ctctggggaa
      241 tcaatcttgt ttgttaatgg aacacttagt tttacaggga atctaaattc tataatgtca
      301 gtattatatg aaggaggtag tattgtaata agttcaggtc ttaattgtaa atcatggata
      361 aggacaataa aacaatataa tgtaactaac atatacctta tacctacaaa acttcaactt
      421 ttggttaaac acttaaaaga accagtactc aaagttgtaa gtatttttac agggtctcaa
      481 ttattatttg aagatactgc aagaaatcta aaaaaatata tgccaaagtc tgaaattatt
      541 ttatactatg gggcaagtga attaaattat attacatatt tatgctatga tgaactaatt
      601 gaaaaacctc taagtgtagg cagaccattt ccaggaatag atatatatat tagagaagga
      661 aaaatttttg tgaatacaga atatgcagta tataatgcaa ccaaacctta ttctgtcaat
      721 gatattggat attatgataa tgatggatac ttaatatttg aaggaagaag tgatgacatt
      781 ataaatattg gaggatttaa agttagttct actaagattg aaaatgaagt taaaaaaatt
      841 cctcaaattg aagatgcaat tgtattgccg tactcagaca ctataagagg aaatcaaatt
      901 gtattatttg taataactat agacaaaatt acgaagaaag atttgttgat taaaatgaaa
      961 gataatctaa tgaaaaatga aatcccaaag aaaattatct ttttaaattc atttccatat
     1021 acctcttctg aaaagataga tagattagca ttattaaaaa tgttataagt ataatattta
     1081 gtaattttag tgaaccaatg tattgataaa aacatattgt atatagtaaa ggaggcttac
     1141 aatatgtcaa atcaatacga tataactgtt attgggggag acctgcgaca agtgtacatg
     1201 gtaaaaaaac taattaataa agggtattct gtagtcactt atgggatatc aaatgaaatt
     1261 ataaatggaa tcgttggaaa atcaacttca ctagctgaag cactatccaa aagttccatt
     1321 attttatgtc caattccatt tacaaagaat caagttaata ttgaaaatac ggatgattgt
     1381 cttgatgcta ctattgataa cttgctaatt aatttatctc ctaatcaaat attatttgga
     1441 ggtaacatac caactaaagt ttgtaaattt tgcgagcaac actctattca gatttatgat
     1501 tttatgaaaa tgaatgatgt tgctatttta aatgcagttg ctacagctga aggggctata
     1561 gcagaagcta ttaaaagaag tataatcaat atacatcaaa gtaactgttt agtattaggt
     1621 tatggaagat gtgcacaaat acttgcaaaa aaattatacg ctttggatgc aaatgtatgt
     1681 gttggcgcaa gaaataatga tgcaagaagt acggctaatg catttggata ttccagtatt
     1741 tctttaagtc aacttgatga aaaactttta caatttgatt atatttttaa caccatacca
     1801 gagtgtattt taacgaaaga aaggttagaa aaagtatcac aagaagtaac tattatagat
     1861 atagcttctt ctcctggagg agtagattat tctgttgtta aagaagcagg gattaatgcg
     1921 actctttgcc ttgggcttcc tggtaaatat tcaccaaaga catctggtga aattcttgtt
     1981 aacgctatag aaaatattat aaatgaaaga agggattaga aatgagactt gaaggactta
     2041 caattggagt aggatttact ggttcgtttt gtacttatga taaaatattt atagagttag
     2101 agaatttggt taaagaagga gctaatgtac acactatatt ttcagatgta tcacaaaata
     2161 tcgattgtag atttggaaat tctgaagagt ttatgaaaaa agcatatgaa cttacaggaa
     2221 ataaacctat agttacgatt gaagaggcag agccatttgg acctaaagga attgctgata
     2281 ttataataat agctccttgt actggaaata ctgctgcaaa attagcaaat ggtattacag
     2341 attcaccagt attaatggca gcaaaaggtc atcttcgtaa tgataaacca ctagtaatat
     2401 caatatcaac gaatgatgct ctaagcttta atttcaaaaa tattggcatt ctccttaatt
     2461 ctaaaaatat atattttgta ccttttggtc aagataattg caaagcaaag cctaattcta
     2521 tgattgcaca tactgagcta ataattccaa ctatagaact tgctctagag aacaaacagc
     2581 tacagcctgt tataaaaagt ccacatcaat agaattggtt cttataatat tatgtattag
     2641 ataaaatact agcaaagagg ctgtctcaaa atagagagaa gcctcttttt cttgttgaaa
     2701 tgatgaaaat atatttaatt tcatacaaaa aaatgcctca tggactaaaa agtctatttt
     2761 gagctaatct ccgtaatttt tggtatatta aaattattta cttaatccaa tttttaaatt
     2821 agaatttata tcgttttgac tcttcaaata tcaatctaac tgttgttgat acaaaagaag
     2881 aggacataat taaaacaaat accgatagtc ctctaataaa caaattatct acataataag
     2941 ttactattcc aatacctcca atcgttaaaa ttacacttat aatcaaagaa aaaacgctaa
     3001 ttgtttttaa ttttttcatg cttatcctcc ttcttataaa cttcaaattt atctatataa
     3061 tattaaatat attttatttc atagtatata aaaatcatga atatat
//
LOCUS       DACXMZ010000312         3098 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.313, whole genome shotgun sequence.
ACCESSION   DACXMZ010000312 DACXMZ010000000
VERSION     DACXMZ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.313"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            104..1546
                     /locus_tag="JD016_18405"
     CDS             104..1546
                     /locus_tag="JD016_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HAU5001591.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1588..1941
                     /locus_tag="JD016_18410"
     CDS             1588..1941
                     /locus_tag="JD016_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001592.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKFYYKIKTFKN"
     gene            2132..2953
                     /locus_tag="JD016_18415"
     CDS             2132..2953
                     /locus_tag="JD016_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HAU5001593.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT         1196 a          315 c          551 g         1036 t
ORIGIN      
        1 acaaatatat aattattaaa ttataataaa tgactatagc gtagatgtaa tactatgtta
       61 tagtcatttt tgaattaaac ttgatatatc aagggggaag aatatgagaa caattagaag
      121 agttttagca ttaggtttaa cattagctat atttctaata aatgtaccta atgtagatgc
      181 attaacatca gatacaatta aaggtaacaa tatatatgag acggctggat taatagcaga
      241 taaaaagagt tatgatacag caattatggt aaatatggat aattctatag cagatggtct
      301 ttcagcaagt ggtcttgcag gtgctgttga tgctccaatt ttgcttgcac aaaaaaataa
      361 gataccaaat gaaacaaaac aaagattaaa aaatgttaaa aagatataca taataggtaa
      421 agaattatca ataagtaagt cagtagaaac tgaactaaaa aacacagggg cacaggtaac
      481 tagattgggt ggagatgaca gaataaagac tagttatagt gttgctaaag aggtaaatgg
      541 tatcaaaaaa gttgatgaag tgatattaac taatgcatat aaaggagaag cagatacaat
      601 aagtgctgca cccgtatcag taagagatat agctcctata gtacttacag atggaaagag
      661 tgtgcctttt tcaacaagca atgtaaaaac atatgcagta ggtgggagta tatcaatgag
      721 cactagttta gttaataaaa ctaatgctaa aagacttggt ggttcagata gatatgatac
      781 aaataagaaa gttataaaag agttttatcc agatgcatca gaattttatt tgagtgatgg
      841 atatgattta gtaaatgcac ttacaggttc tacaattgct aaggaaaatc caattgtatt
      901 ggtgtcagaa agtagtgata agtctatatt agcaggagca gataagatta ctagattagg
      961 ttcaataagt gacagtgtgt ataataagtg tgtttctgct gcacaaaata atggcgattc
     1021 gtctacaaaa ggtgagtcac ctatgaaaaa tgagacaagc atattaggtc agccaacagc
     1081 tagtttagaa gcgtgcttaa aatgggcaaa atctaaaaaa gctaatgatt tatttataga
     1141 gttaatacca atattatatg atactgctgt tcaggaaggt gttaatcctg ttttggcagt
     1201 agctcaatct gcaaaagaaa ctggtttttg taattttggt ggagtattag atgcatcatt
     1261 taagaatcct tgtggactta aaacttctgt aggtggttct gatactgata agaatgccca
     1321 ttcaagattt gatacttggg aagaaggaat attagctcaa attcaacatt tgtgcttata
     1381 tgcaggacaa gatggttacc cactttcaaa tccagtagac cctagacatg aaaaatcttt
     1441 atttggtaag gctaaaacag ttgaaagttt atctaataat tgggctggag gtcaatatgg
     1501 acaagattta gtaagaatga tgggagaaat tgaggcaaca aaataattga agattatcaa
     1561 atatccaata aataaggatt gattattatg aaaaagagta atatttttag tatcatattt
     1621 cctacagtca taatggtgct aatgttttta acttttgcta cagatatatt taatattcca
     1681 gatattcaat ctaaaagtat ttttgttgtg ggaatggttt tgatttttcc aatttctttt
     1741 ttgatacaag ggataatttg tgtattaaat aagactaatt ggattttatc tcttattgtg
     1801 tctttagtaa cttatatagt attaatgtat atatttttaa atgattctgc atatatatat
     1861 gttttacttt atacagcttt ttatatgatt ggacatataa cagctaaatt ctattacaaa
     1921 attaagactt ttaaaaatta aattgaagta tataaaagta acattaggag aagtatcctt
     1981 aatgattttc taaaatcaga aataggtgct tctctttttt gtaaaaataa aagtatgtaa
     2041 ataaaattta accattataa ttttctttta acaaatgtat acaataaact tgttgtataa
     2101 ttaagataga taaaataaag ggagggataa tatgaattta atagataatc atattcatac
     2161 taatttttca agtgatggaa aggattccat ggaagataca ataaaaaaag ctataagtat
     2221 tggtgttaga tatttgactt ttacagacca tttagaacat gatgaagata gaggattttg
     2281 cataaattat aatgactatg ttccagtatt taataagttt aaagaaaaat ataaaaagga
     2341 catagagtta ttactaggtg ttgaagtggg ttacagaaaa catctaaaaa atgaaataga
     2401 agaaataata aattctaatc cttttgattt tgtattgtgc tcaactcata caattgacaa
     2461 tgtacctgtt ccatcaaagg cgtattttaa aggtcttagc aaagaagatg cgtattataa
     2521 atattttaat agcatacttg aaactaatcg tgaatttgga gattacaata tatatggtca
     2581 tttggattat atatctaggt atggaatata ttcagataat agagttatat acaatgactt
     2641 taaagatgtt attgatgagg tcttaaagtc aataatcaat aatgggagtg gtatagagct
     2701 aaatacttct ggttatagat atggattaaa tgctatacat ccaaatgaag atattctaaa
     2761 aagatataaa gaacttggag gatttatagt tactgtaggt tctgattctc atagggtaga
     2821 agatatatgt aaagattttg atgtggccta tgatatgctt aagtatcttg actttaaata
     2881 tgtatcttta tttaaagaaa gagaaactta ttttgtaaat atagaaaaag ttaaatctaa
     2941 caatatagct tagagtgtgt tttgaagtaa attaatggat agttaaaaag ttaatagtta
     3001 taatattgta tcctctagaa aaatactaga ggtttttatt ttgctaaaat aactaaatag
     3061 aattaaattt aaatagtata gcttatttta aattgaat
//
LOCUS       DACXMZ010000313         3093 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.314, whole genome shotgun sequence.
ACCESSION   DACXMZ010000313 DACXMZ010000000
VERSION     DACXMZ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.314"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     misc_binding    28..290
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            366..3032
                     /locus_tag="JD016_18420"
     CDS             366..3032
                     /locus_tag="JD016_18420"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAU5001594.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPEKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         1234 a          348 c          609 g          902 t
ORIGIN      
        1 aatatttgta tataataata tgtaatatta aagactgaga agaagaggag taagtaaata
       61 ctactaaaag agaggaaaat tcactggctg taagattttc taagtataag atttactgaa
      121 tgtagctttg gagtttctaa actgaactat aagtaagttt agacggttag aaccgttaaa
      181 ttctttgaga gtcaaagttg ataaaataac attaaccaat aatttaatta tatgttaatg
      241 tattttaagc tttggaatta aggtggtaac gcgagctttt cgtccttttt aaagaggatg
      301 aagagctctt ttttatttct aaagatattt aaaaaattta tttataaaat acaggaggca
      361 ggacaatgga aaatacgaat ttatcaaaaa cttataaccc aaaagatttt gaggctaggc
      421 tatataaaaa atggatggat gaagggtatt ttaaatctaa accaaatcca gataaaaaac
      481 catttacaat aatgatgcca ccaccaaata taacagggca attgcatatg ggacacgctc
      541 ttgaccatac acttcaagat atattaatca gatggaaacg tatggatgga tatgaagctt
      601 tttggcttcc aggaactgac catgcttcta tagcaacaga agttaaagtt gttgaaagaa
      661 taaaaaaaca agaaggcaag acaaagtatg aaataggtag ggaagaattt ttaaagagag
      721 cctgggaatg gaaagatgaa tttggaggaa aaatatctaa tcagcttaaa caactaggtg
      781 attcttgtga ttgggataaa gaaagattta ctatggatga agggtgtaat gaagcagtta
      841 tagaattttt tgtaagccta tatgaaaaag gtcacatata cagaggaaat agaataataa
      901 actggtgccc agattgcaaa actactttat cagatgcaga agttgaacat gaagaacacg
      961 acggaaactt ttatcatata aagtacccac taaaagatag tgaagacttt ttagaaatag
     1021 ctacaactag acctgagaca atgataggag atactggtat agcagtaaat ccagaagatg
     1081 atagatataa acatttaata ggaaaaacag caatacttcc attagtagga agagaacttc
     1141 caatagttgc agatagttat gttgatttag agtttggaac tggtgcagtt aagatgactc
     1201 cagcacatga ccctaatgat tttgaagtag gattaagaca taatcttgag cagttaaata
     1261 ctatgaatga agatggaact atgaatgaag tttgtggaaa atatgaaggt atggatagat
     1321 ttgagtgtag aaaagctatc gtagcagact taaaagaaca aggttaccta attaagataa
     1381 aagagcataa tcataatgta ggaacttgtt atagatgcca tacagtagtt gagcctagat
     1441 tatcagagca atggtttgtt aagatggaag aacttgcaaa accagctata gatattctta
     1501 aaaaaggaga attagaattt gttccagata aatttgataa aacttattta caatggctag
     1561 aaaatataag agattggtgt atatcaagac aattatggtg gggtcatcaa ataccagctt
     1621 attattgtca agagtgtgga gaaatagtag ttgctagaaa aatgccagag aagtgtccta
     1681 aatgtggaag cactcatttt aaacaagatg aagatgcctt agatacttgg ttcagttctg
     1741 cattatggcc tttctctact ctaggatggc caaataagac tgaagcttta gattattatt
     1801 atccaacaag tgtacttgtt acaggatacg atataatatt cttctgggta gttagaatgg
     1861 catttgcagg tatgttctgt atgaatgaaa agccatttga ccatgttttg gttcatggat
     1921 tagtaagaga ttctcaaggt agaaaaatga gtaaatcttt aggaaatggt atcgacccat
     1981 tggagataat cgagcaatat ggagctgatg cactaagatt tacattaaca actggaaatt
     2041 ccccaggaaa tgatatgaga ttctatatgg aaagagtaga atttgctaga aactttgcaa
     2101 ataagttgtg gaatgcatca agatttgtat ttatgaatat tgatgaagat ataattaaaa
     2161 acatgactag agaaagtgtt aaagaggact taactctagc tgataaatgg ataatatcaa
     2221 gagcaaataa tattgtaaaa gaagcaacta ataacatgga taagtttgat ttaggaatag
     2281 cacttcaaaa gatatacgat tttacttggt cagaatactg tgactggtat attgaaatgg
     2341 ttaaacctag attatatgga gaagatgcaa atgctaaatc tgcagcatta tatacattaa
     2401 cttatgttct tgaaaaaata ttaaaattat tacatccata catgccattt ataacagagg
     2461 aaatatatac tcatcttcct actgttgaag gatgtataat agtatctgaa tggccaaaat
     2521 ataatgaaga agataacatg gctgaagaag aagatatgat gaacttattg atggaaggta
     2581 taagaagtat aagaaatgtg agagcagaaa tgaatgtacc accttctaag aaagctaaat
     2641 tgataataat cccaagtgaa gaaaaaatag aagctattga gcttggtaaa gactatttta
     2701 taactttagc atctgcttca aatgtagaga tagcaaaaga taaatctaat gttccagaag
     2761 atgcagttgg agtagttatt gatggtgttg aaatatttat acctcttaat gaattagttg
     2821 attttgaaaa agaaattgag agattatcta aagaaaagaa aaagttagaa ggcgaaataa
     2881 agagagttaa tggaaagctt gcaaatcaag gtttcttagc aaaagctcct gaaagtttaa
     2941 ttgaagaaga aaaagcaaaa aaagaaaaat ttgaagagat gattaaatct gttgaggaaa
     3001 gattaacaaa tttagaatct aaaataaaat aaatataaga attagtaaaa acatacaaaa
     3061 agtatatatt ctatactttt tgtatgtttt tct
//
LOCUS       DACXMZ010000314         3016 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.315, whole genome shotgun sequence.
ACCESSION   DACXMZ010000314 DACXMZ010000000
VERSION     DACXMZ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.315"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(323..1381)
                     /gene="rsgA"
                     /locus_tag="JD016_18425"
     CDS             complement(323..1381)
                     /gene="rsgA"
                     /locus_tag="JD016_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAU5001595.1"
                     /translation="MKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAVCVN
                     GVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRKAAG
                     KVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCDNVY
                     RKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINCLMG
                     GEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKSFAD
                     IEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSEYLA
                     KKEKKFKNISKINKKIRR"
     gene            complement(1387..1788)
                     /locus_tag="JD016_18430"
     CDS             complement(1387..1788)
                     /locus_tag="JD016_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HAU5001596.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      2584..2680
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2734..2910
                     /locus_tag="JD016_18435"
     CDS             2734..2910
                     /locus_tag="JD016_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001597.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
BASE COUNT         1047 a          522 c          381 g         1066 t
ORIGIN      
        1 ttagcaatat tgcagttttg ctcttaagaa acatctctat catacataag catatactct
       61 attatgtatt agtataaata tgacttttct tatcttacat aagacctctt tataaaggag
      121 cctatcgtaa caaatatgta tacattatca catgtatata tcatcatata aaaaatcaaa
      181 ctttattaaa aaaattaata aattgcttta aataatacca caaaccaaat ttgccatcaa
      241 aatcacatta aagtaacaaa ttgatggcaa atttaatata ttaatagtat atagactcat
      301 ataatgaaca aaaaaactac acttatcttc taatcttctt atttattttt gagatatttt
      361 taaatttctt ttcttttttt gctaagtatt cgcttttatt gtctgcaaat actgcttcag
      421 ttttaagcct acgataggac tcccatcttt taggcgaaag ttcttgatga gcaatagctt
      481 cccagatagc acagcctggt tctgaggtgt gagtacaatt acgaaaatga cattttccaa
      541 aataagcttc tatatctgca aaacttttat ctaaaccttc tgaaacatcc cacattccca
      601 actctctcat acctggtgtg tcaataacaa taccaccacc tgaaattaaa aatagctctc
      661 gtctagtcgt ggcatgtctt cctttatcat catctatgcg aatttccttt gtatttaaaa
      721 attctccacc catcagacaa ttaattaatg aagattttcc aactcctgaa gaaccaagta
      781 aggcaacagt acagcctggt ttaatatact tagaaatctg tttataacca tctggctcta
      841 tagatgaggt aacaactaca tctacaccca aggctatgga attaactact tgtaatttac
      901 gatacacatt gtcacataag tctgatttag taagtactac tactgggata gctccacttt
      961 cccatgttaa agacaaatac ctctccaatc tatgaatgtt aaaatcctta ttcagtgaca
     1021 tgcacaaaaa aacaatgtca atattagctg caaccacctg ctcctcattt acctttccag
     1081 cagccttacg cacaaacaag ctttttctat gaagcacatg atgaataata gcattaccat
     1141 ttttattatt gcaacgatcc aacataacga aatcaccaac tgtgggaaaa tcagattgta
     1201 tagtagcctc aaaacgcagc tttcctgaaa cctctgcaaa cagtacacca tttacacata
     1261 cagcttgata aaagttcttt tcctgcgaga taattcgagc aaggtataaa tcatgatatt
     1321 ccatactttg agtaataaaa ttcctattta gaccatagtc ttgtaaattg atttgtttca
     1381 taccattcat tctgtttcct ccttttccaa cttttcaatg attctttcta gtgggaaagg
     1441 gggctgtgcc agtggtttta atgcaattga cattaatttc actgaattat catcagctgc
     1501 aatacgatta gaactaagat gtccacagcg tgtacaacga tgaataattg cccactcacc
     1561 attttttctc acccacactc caataggttc catattagca tcacaatctg cagcacggtc
     1621 accaggaatg atatctacat gaagactatg taaacaatgt ggacaatgat ttctatgttg
     1681 tgtaccagct ccttcaggaa ctacaaggca accacaattt ttgcaaataa agttatccat
     1741 acaggaattt tcctgataat aagctcgttt tctattttta taattcattt ttatttcctc
     1801 catatcgagt attttctaca aaaaaagtcc acaggtattt atatctgcct ttttatgtgg
     1861 tatacatatt tacctacaca aaaacagaaa acccctgtgg actagaaaat ttaatatctt
     1921 ctatttcaac ataattatat tcaaaataat actgtgtaaa attaataaaa gtgtagcaga
     1981 ttaaagccca ctacaccaca atttaacaaa gtaatatgta atattattat ttgttgtcta
     2041 atgttagtta tctataaagg cagagtaaga cacaacaatc aaatgtatca aaaatacact
     2101 taaaaattct gattaaacag aacccatatg cgtctccaac tatttatttg ttggttacca
     2161 ttatatataa ctctgacatc aggcaagaca ctccttcact aataattatt attgaaattt
     2221 tatcataaaa tataagtatg ttcaagtata atttaatata ttcatatatt tatgtttgtc
     2281 tcattttctt attctataaa atctagtggt attttaaaat ttaatgttca cctagtatta
     2341 ataataaaaa ttgactgcta ttatttgttt tctcatttta tgcctattgt tcaatttatc
     2401 tcacttaaat taataacaac tgtcttttca tacaaaacct acaatcaagt ttttccatat
     2461 attctacatg catagtctat ttttttgata taaaataacg ttcatatttg taaatttatt
     2521 taattttcaa ataagtatat tgaataataa gcaattctat tgtattattg taattgtaga
     2581 ataaaactaa atcggcaaaa ctagagaaat ttagtgacgc aaagctatag ggactaacgc
     2641 ttataaaata aactatgagt tatgtcagcc agttgccaaa aagagatatt ctttttgttt
     2701 ttatgaaagt tttttagggg gataataata tatatgttta gagataaaat ggatagatgt
     2761 acacatatgt tgactgctta tattggtagt tcatatgatt attgtgattt tatagataca
     2821 caactagatg attttatact agagtacgga gaaaatgtgg tagaatcttg tttgcatcaa
     2881 gtgatggtat tagtaagtaa gtataattaa atagatttgg ttttctagag aacccttttt
     2941 agttttatct actttaaata gccttatgtt agaaagctaa ttaagataga tttctagtat
     3001 tcataatttt attttt
//
LOCUS       DACXMZ010000315         2994 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.316, whole genome shotgun sequence.
ACCESSION   DACXMZ010000315 DACXMZ010000000
VERSION     DACXMZ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.316"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            153..1046
                     /locus_tag="JD016_18440"
     CDS             153..1046
                     /locus_tag="JD016_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase"
                     /protein_id="HAU5001598.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            1489..1980
                     /locus_tag="JD016_18445"
     CDS             1489..1980
                     /locus_tag="JD016_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001599.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYTGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSF
                     KIE"
     gene            1999..2955
                     /locus_tag="JD016_18450"
     CDS             1999..2955
                     /locus_tag="JD016_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001600.1"
                     /translation="MDAMRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYT
                     IIVTNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQKGFYIGRIDASCKK
                     IISFKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLN
                     ISNTLKAPKNLRNILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCIEVEIDYSC
                     KNRSNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCF
                     ICSPVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT         1315 a          263 c          419 g          997 t
ORIGIN      
        1 tgtgatagaa aactatatat tataacaata gtagtgcttt taattaaata tatttcataa
       61 atcttcataa aatcttcaaa atttcgtaaa gaatatgtca caaaagtaag gtatattata
      121 ttcagcagat agtataaggg aggacaatat ttatgaagaa taaaaaattc attgttataa
      181 tactgataat atcaatttta ataggtgtat tagtgaagga atattcatct agagagataa
      241 aaaaggatga tataaatgtc agtaagtata taaaatatgc tgatttagca agtaaaaata
      301 atgcacaagt taactggaag tatgtagctt ccattgttgc tgtgttaaat aaaaataact
      361 taaaaaacgt taaggattca cagatacaag aagtatcaga tttatttgtt aagaattttt
      421 ctaaaaataa taaaataaat aagttaagtg atattttgga tgagttagaa tttagtaata
      481 gacaaaaaag attggttgat aattatatag acaatcttaa ggattatggt ataaaaccag
      541 aaagattgaa gtctgataca aaatatatga agtttattga tgaaattaaa acagaagcaa
      601 tacaaaatta taaggattat aaaatacttc catctataac tattgcacaa gctataatag
      661 aatctagctg gggtaagtca actttggcaa aacaatataa caatctattt ggtataaagg
      721 ctgacgctta ttggaaaggt aagtcagtga ctttagaaac aaaagaacat ttagatacaa
      781 ttatagatga taaatttaga atatatgatg ataaaaatga atctataaaa gaccatgcaa
      841 aatttttagc cacaaataaa aggtataaaa acaatggtgt atttgatgca aaaacgtata
      901 tatatcaagc taaagcatta gaaaaggcag gttatagtac tgcgaaggat gaaaatggca
      961 atagtatata tgcagctcgt ctaatagaat taattcaaca gtataatctt caattaatag
     1021 acagtgaaat tcaaagtgaa atgtaacttt gattaaattt ttattgataa ggctatgatt
     1081 tttaaaatca tagccttatt ttgacatgct tttacttgta attattaaat cgatatcatt
     1141 atctttcaaa atttacttta tactgtaaat caaattattt aagttaaaac ttggaattat
     1201 ttataaaagg attatttact ttatctaaat tctgcactta aagaacatct tttcccttaa
     1261 gactgaccgt tagtctataa aaattataat tacttataaa aaagtgaata gagagatata
     1321 tatataattt tcagaaagac ttttacttgt aaattgaaat taatgaagca aaaaaggatt
     1381 ctttcttaaa agtaaataac aactagatag tgaataataa aaaatggtaa tttgaaataa
     1441 tttttatgaa tagtattaat atatagatac agggagattg atgcgattat gtatgaaaat
     1501 aataaggtaa tatacaacct tgaaagatgt gaaaaactag aaaagactct ttatttatct
     1561 caatttaata catataaaaa ttttaaatta gcttctaaaa aaactgtaat aaattcatta
     1621 gagacatatg tttacaatat gaatattaac aatataagtc ttgttgatac tatagaatgt
     1681 atatcattag aagatgaaca acttacagga aagaagttga ttttagttgg tgatatagat
     1741 attgatgcaa tattagatta tacaggcaat aaaagaaata gaaattctag taaaaaaaat
     1801 acatttaaat taaaaatacc ctttagtaca tttatacaaa tgcctagaaa gatagaaaat
     1861 aaagataaaa ttaatttaaa gtatcttata caagatatta cttcatctat tttagatgat
     1921 aatttattta ttagtgttac tgcaattata agttatgaaa atagtttcaa gatagagtaa
     1981 tctttaaatt atagaaaggt ggatgcaatg agaataacga aaactacttt taatgatgta
     2041 ttttcaaatg atgcaaatgg aagtattata aaagtagaaa aatatgttga taaaaaatgt
     2101 gcatctgtat ttgagatttt gacctacact attattgtta caaatacaag taggtacaaa
     2161 actggaaata tatttttcaa agattatatt tctaaatata tagagtttat aaataatact
     2221 gtaaaagtta atggcattat aaaacgtgga ttagacccac agaaaggttt ttacataggg
     2281 agaatagatg caagttgtaa aaaaataata tcttttaaga gtgtagtttt accaaattct
     2341 gcacatagga ttataaagaa tagtgctaat atatactatt attataagtg taatctagat
     2401 aaattcccaa caagaatatc aatagaaagt aatagagttt atactaatgt aaataaaatt
     2461 gtattcaaac aattgaatat aagtaatact ttaaaagctc ctaaaaatct aagaaatata
     2521 ttaaaagtaa atgtaaatag taaaatattt gatgttaaac ctatgaaaaa tcttataaac
     2581 aaaggtagta atttgaaatt atgtaacttg atagtttttg ggtgtataga agttgaaata
     2641 gactatagtt gtaaaaatag gagtaacttt aaaacagtaa ataagtctaa tgacattaaa
     2701 aaagataagt attataaaaa tgatatacaa aataacaatg aaaatataaa cgattgtaaa
     2761 aagcaaataa gttttggtga taataaggta aaaaaaataa caaaaacttt tggattttct
     2821 tgtttcattt gttctccagt tggaattatt tatgaagata tgaaaaatat aaatataaaa
     2881 attgaacata catcaataaa tgaattaaat ccagaagaat tatttgtaaa tacatcactt
     2941 ttattgtatt attaagtatt ttaaagctaa aacttaaact tattaaaata gcat
//
LOCUS       DACXMZ010000316         2966 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.317, whole genome shotgun sequence.
ACCESSION   DACXMZ010000316 DACXMZ010000000
VERSION     DACXMZ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.317"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            161..886
                     /locus_tag="JD016_18455"
     CDS             161..886
                     /locus_tag="JD016_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP--phosphocholine cytidylyltransferase"
                     /protein_id="HAU5001601.1"
                     /translation="MKAIILAAGLGTRLKPLTSECPKPLVKVNGKPMIETQIECLLEK
                     GIKDIKVAVGYLKEKFYYLAEKYNVELIYNNKYDIYNNIYTMYLLREHLPDSYVIEGD
                     VYICNNFIDPDIKESTYFTLYKENFNNEFIFKFDENNKVYDMYVGDNKGYILCGVSYW
                     SLTDGNIIAKKLEETIENGDFESLFWDDVVMKNKDALNMYIQKIDFKDCFEIDNEKDL
                     RFSEKCIVQNNKFTMKKILQYSK"
     gene            904..>2966
                     /locus_tag="JD016_18460"
     CDS             904..>2966
                     /locus_tag="JD016_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAU5001602.1"
                     /translation="MGILDEIMNQEHFDIKTSLDYINEYEKLLPFDSEIHTARAVTYF
                     FGEEYKLAEEHIRKALKFRPNNYDNNFYLANILIAMGEYPDAIKSAVLSVTFLKHFAK
                     IQGYDYINDKLFYYNEQVKTLIEYCIENIKDTNVLEDLKRFFGFIELSIAKFPVCFYG
                     DKWNILVGEYLNKDLNSEYNDYICFYSDTYNNMLSDFQKKLYTVINNSEISSICPIES
                     FKSMTAKEFSLDNGKYIIPIATTEYNQVIDFKIKNTNFKSQQVDPPGFFKYYRVNNGI
                     SLNSDKDVIIGKPIKLGFKEKNKKLVFTIFIDALSQKYLLETKYKSMPYTKAFFDKGI
                     IFENCYSAGEWTYPSLTSVVTGLYTTNHHFLHSSAGYKYPDDIDLFSEIMKKNEYFTA
                     NISGTYALAPYSSGFRGFDRTVFKNSLGFSDSVIINDAIKHIESFKETNQFVFMSLSD
                     VHKILDDIENYSVNYDIVNQTSLDIETSLSKRSSFVSAWQKYDEEAIKKYDVALRELD
                     RQLKGIYDYILQNYSEEEYLISIFSDHGASFLGKDDFLLKSNITNSVLMVRGSGSASN
                     GNTEYISNMDLIPIILKLANIEHDLSKYDCVLPKTLGGKGREFTYSESIYPGQTYKAM
                     VNNDKYCYILETKGITDIDGTINFDEYSDSVFDKKEDKEVKDNDILEYYREIVYNHIK
                     RNIKY"
BASE COUNT         1187 a          273 c          486 g         1020 t
ORIGIN      
        1 tttagacctt tagaataaat aaaaataaac attaggttta aaatgaaatt taatatatac
       61 taataaaaaa tttctatcgg aatatttgga gattttataa gtgatactaa cttaaaacct
      121 ctaaaacatt acaatattca attggaaggt aggattaaat atgaaagcaa taattttagc
      181 agctgggctg gggacacgat taaaaccatt aacttcagag tgcccaaaac cattagttaa
      241 agttaatggg aagccaatga tagaaaccca aatagaatgt ctattagaaa aggggataaa
      301 agatataaaa gttgcagtag gttatttaaa agaaaaattt tattatttag cggaaaaata
      361 taatgtggaa ttaatatata acaataaata tgatatatac aataatattt atactatgta
      421 tttattaaga gaacatttac ctgatagcta tgttatagag ggagatgtat atatatgtaa
      481 taactttata gatccagata taaaggaatc cacttatttt actctttata aagaaaattt
      541 taacaatgag tttatattta aatttgatga aaataataag gtttatgata tgtatgttgg
      601 tgataataag ggatatatat tatgtggtgt atcctattgg tcattaacag atggaaatat
      661 tatagcaaaa aaattagagg aaacaataga aaatggagac tttgaaagtc tattttggga
      721 tgatgttgta atgaaaaata aagatgcttt aaatatgtac attcagaaaa tagattttaa
      781 agattgtttt gaaatagata atgaaaaaga tttgagattt tcggaaaaat gtatagtgca
      841 aaataataaa tttacaatga agaaaatttt acaatactct aagtaggtta ggaagggatt
      901 tgtatgggaa ttttagatga aataatgaac caagagcatt ttgatataaa aacatcacta
      961 gattatatta atgagtatga gaagctacta ccatttgata gtgagattca cactgcacgt
     1021 gcagttacat atttttttgg agaggaatat aaacttgcag aagaacatat tagaaaagca
     1081 ttgaaattta gaccaaataa ttatgataat aatttttatt tagcaaatat attgattgct
     1141 atgggtgaat atcctgatgc cattaaatca gctgtgttaa gtgtgacatt cttaaaacat
     1201 tttgctaaga ttcagggata tgattatata aatgacaagc tattttatta taatgaacaa
     1261 gtaaaaactc ttattgagta ctgcattgaa aatataaaag atacaaatgt acttgaagat
     1321 ttaaaaagat tttttggttt tattgaacta agtattgcta aatttcctgt ctgcttttat
     1381 ggggataaat ggaatatttt agtgggagaa tatttaaata aggacttaaa tagtgagtat
     1441 aatgattata tatgttttta ttctgacact tataataata tgttatcaga ttttcaaaaa
     1501 aaattatata ctgttattaa caattctgag atatcttcaa tatgtccaat agaaagtttt
     1561 aaatctatga ctgcaaaaga atttagttta gataatggga agtatattat tccaattgct
     1621 acaactgaat ataaccaagt tattgacttt aaaattaaaa atactaattt taaatcacaa
     1681 caagtagatc cacctggttt ttttaagtat tatagagtaa ataatggcat tagtttaaat
     1741 tcagataaag atgttataat tggtaagcca ataaagctag ggtttaaaga aaaaaataaa
     1801 aagttagttt tcactatatt tatagatgct ttatctcaaa aatatttatt ggaaaccaaa
     1861 tataaatcaa tgccttacac aaaagctttt tttgacaaag gaattatatt tgaaaattgt
     1921 tattcagcag gagagtggac atatccatct ttgacaagtg tagttacagg gttatataca
     1981 actaatcatc attttttaca ttctagtgca ggatataaat accctgatga tatagatttg
     2041 ttcagtgaga ttatgaaaaa aaatgaatat ttcactgcaa atataagtgg tacatatgca
     2101 cttgcaccat atagcagtgg ttttagagga tttgatagaa ctgtttttaa gaatagttta
     2161 ggattttctg attcagtaat aattaatgat gcgattaagc atattgaatc atttaaagaa
     2221 accaatcaat ttgtgtttat gtctttgagt gatgttcata aaatattgga tgatattgaa
     2281 aattatagtg ttaattatga tattgttaat caaacatctt tagatataga gacatcttta
     2341 tcaaaaagga gttcatttgt aagtgcatgg caaaaatatg atgaagaggc tattaaaaaa
     2401 tatgatgtgg cgcttaggga attagaccgt caattgaaag gaatttatga ttatatatta
     2461 caaaattact ctgaagaaga gtatttaatt agtatttttt cagatcatgg agcttcattt
     2521 ttaggtaaag atgatttttt attaaaatca aatataacta atagtgtatt aatggttaga
     2581 gggtctggaa gtgcctctaa tggtaataca gagtatataa gtaatatgga tttaatccct
     2641 ataattttaa aattagcaaa tatagaacat gatttatcta agtatgattg tgtacttcca
     2701 aaaacactag gaggtaaggg gcgtgagttt acctatagcg aatcaatata tcctggtcag
     2761 acttataaag ctatggtcaa taatgataaa tattgttata tattagaaac aaaaggcatt
     2821 actgatatag atggaaccat taattttgat gaatacagtg actctgtttt tgataaaaaa
     2881 gaagataaag aagttaaaga taatgatatt ttagaatatt atagagagat agtatataat
     2941 catataaaaa gaaatattaa atacta
//
LOCUS       DACXMZ010000317         2950 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.318, whole genome shotgun sequence.
ACCESSION   DACXMZ010000317 DACXMZ010000000
VERSION     DACXMZ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.318"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            42..1229
                     /locus_tag="JD016_18465"
     CDS             42..1229
                     /locus_tag="JD016_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAU5001603.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPKKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            1255..1947
                     /locus_tag="JD016_18470"
     CDS             1255..1947
                     /locus_tag="JD016_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAU5001604.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCREGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(2164..2466)
                     /locus_tag="JD016_18475"
     CDS             complement(2164..2466)
                     /locus_tag="JD016_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HAU5001605.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(2488..2898)
                     /locus_tag="JD016_18480"
     CDS             complement(2488..2898)
                     /locus_tag="JD016_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAU5001606.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
BASE COUNT         1013 a          464 c          347 g         1126 t
ORIGIN      
        1 gtatattatt tgcattataa aaataccata cggaggataa tatgaaaaaa atatctaatc
       61 aaaaattact tttactttct attgttacat ttttctggtt ttcacaatat gtatatatac
      121 ctttccaaaa tccatattta agtgcatgta atgtatctac aaatatgatt ggtataataa
      181 taggtgcata tggaatctca caatgtttat taaggcttcc tgttggagta tttgctgatt
      241 ctataaataa acataaaata tttatactgt ctggaatttt ctttgctggt ttagcatctc
      301 ttataagagt cttttctcct ggaggcaatg gttttcttat agctaacatt ttatctggaa
      361 tatcatcatc aacttggatt tcttacatgg ttcactatac caactttttt tctacagaac
      421 atcaacaaaa agctacaagt acgataatat tggtaaacaa tctaggaatg ttgattggat
      481 ttgttatgag tactttattt tatgaaaaat taggaatgac agccttatgt atcatgagtg
      541 tattgtcttc agttattgga acgattctag ccttaaatat aaaaaatcat aattcaaaaa
      601 aaatacctaa aaaaatatca attaaaaaat atttatctat ctgcaaggat aaaagacttg
      661 tatttttttc ctttatggca ttaattcaac agggtattca aatgtctaca acaatgtcat
      721 ttactactca aattcttaaa aacttaggtt ctagttctac cctcataggt ctatcttcta
      781 tattttacat ggtgtgtgct gttgcttctt ctggctttgc ttctactaaa ttttgtacta
      841 agtatgggcc aaaattttgg attcctttag tttttatttt agtttcagcc tattgctttt
      901 tagttccaac aataaaaaat attaatataa tatttgtact acaagtacta ccaggtatat
      961 ctacaggtat actcctttct taccttacta gcgagtctat gaaaaatgta ccaaaacatc
     1021 agaaatctac tgcaatgggt ttttttcaag caatttatgc tataggaatg tctgtatttc
     1081 caatgatagt tggtaaaata tcggaatata cttcaataca atatggatac tttatcttgg
     1141 ctattatagc tattttggcc tctatcctgt cttatacata ttataaaaaa gtagatttaa
     1201 ctcttaacta tgatataata caatcataaa aaaattatat gtttaggagt ttttatgaaa
     1261 agcttaaatg ccactgaaaa aattattgat tatattaaat taaacattct aaatggaact
     1321 tttaaaatta atagtaaatt accaagtgag agaaagattg ctgacctatt taacataagt
     1381 agaatacctg taagaaatgc cataaatata ctctgtaggg aaggaatact tagagctgtg
     1441 ccttattcaa gtcctatagt tgaaggattt aaaaaagttg acctatttga tgatggtgaa
     1501 atctataaaa atcataacat acaagaattt tatgtagaat ctttaagagc aagacaactt
     1561 atcgaaagcg aagctactaa actagcaatt ttaaatgcta catctaaaga acttcaaaaa
     1621 ataaaatata cttacctaaa gtcaatagag gaattggata aggtttctca tggtttgata
     1681 gaagaatgtt atgatgctga cttgcaattt cacaaagaga taattttagc ttctcataat
     1741 ccaattttta taaaatatta tgagttaata cctaaaacag tttcttctaa ccaatacttc
     1801 ggttttaaat atagaaattc tctcaaggat atgattagcc accataacaa gataatgatt
     1861 gcatttgata gtaaagactc ttacttaggt tatacatcaa tgtacaatca tttagaagat
     1921 gtaattcagt tatttcaaca tgattaaact tatattttac cttaaatgca actaacatat
     1981 aaaaatggag tgtctcaaag tgaacttttt agttatgagg tactcttttt tgtatgaaat
     2041 caaatatatt ttcatcattt caacaatgaa aaagactctt atctatattt tgatacaacc
     2101 tctttatttc ttgtgtaaaa tatataaaaa ggcgtcgctc tattcttagt gcgacaccca
     2161 cgattaattt aagtctatta aaatttcact tactatagat tttgcatcct ctattatata
     2221 ctttggatca acttcataaa attctctatc tataaatcta tcaatcttct ctatgctttc
     2281 tatagaatca cttgtaacaa ataacactat ggatgcatct tctatatcat aaatagaaag
     2341 ttcatttttt atattatcat tttcttgaat ttcataaact aaatcaaatc ctaaatcatt
     2401 tgcagctgtc ttaagagaat ttaaacttct ttcactttca caaactgcta ctatcttttt
     2461 attcatattt caaatacctt tctaattcta ctccggtaca atcatcacta tacattttgc
     2521 ttgtttaact acacttgctg ttactgaacc tatcattctt gtaagacctt ttttagtaga
     2581 ttttgtcata actataaaat ctacattatc ttcttttgct tttctaatta tctcatctcc
     2641 tggatatcca aaagtaaaat gtactttaac atcatactct cccattatct ctgctgcttt
     2701 atccaagatt cttcttccta aattttcaga attttttatc tcttcagcca gggatatgcc
     2761 atcaataaat actagttctt ttacattcat tatttctact tcaacttcat cttttttaaa
     2821 tattcctttt ataaattcca aagaatgcat acttctttct gtcccatcaa ttggcactaa
     2881 tattttcttt ttattcatgt cacatcttcc tttctcactt taggataatt aaataaaata
     2941 tataataaat
//
LOCUS       DACXMZ010000318         2947 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.319, whole genome shotgun sequence.
ACCESSION   DACXMZ010000318 DACXMZ010000000
VERSION     DACXMZ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.319"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            109..477
                     /locus_tag="JD016_18485"
     CDS             109..477
                     /locus_tag="JD016_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAU5001607.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            480..851
                     /locus_tag="JD016_18490"
     CDS             480..851
                     /locus_tag="JD016_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAU5001608.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(995..1450)
                     /locus_tag="JD016_18495"
     CDS             complement(995..1450)
                     /locus_tag="JD016_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001609.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1489..2754)
                     /locus_tag="JD016_18500"
     CDS             complement(1489..2754)
                     /locus_tag="JD016_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HAU5001610.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1089 a          407 c          407 g         1044 t
ORIGIN      
        1 attggaatta tttataaaat accccaaaat ttaaaaataa tggttgaaaa atctaattaa
       61 gagtactata atttaattaa gtaattactt aatcaaaacg aggtacttat ggaaaacttg
      121 gttaaaatat ttaaagcatt gagtgatgaa actagactta acatacttat attagtgtcc
      181 aagagaaata tatgtcaaaa ggggatttct aaatatctag ggatttcaga ttctgctgtt
      241 tcacaacata taaaaatatt aaaagatgtt gggataataa caggatataa agaaggatac
      301 tatgttttat atcatattaa taaagaatca tttaatatat gtgtagattt tataaattct
      361 atgttaagta attctagtga aagctttata gatgtatttg atgtaaatac aatacatcta
      421 ggatgcagta aagaatgtaa atctataaaa aaatgttgta agagaaggga gaaataataa
      481 tgaaagtatg tataccagta gaggagaata aaggattgga tagtaaacca tatggacatt
      541 ttggttctgc tccaattttt gttgtgtgtg atttagaaag tggagaagtt aaatctttag
      601 ataatggaga tttagatcat gaacatggaa aatgtcaacc attaaaagca ttgtcaggga
      661 cagctgtaga tgcagtagtt gttggtggaa taggacaagg agctataatt aaattaaatg
      721 gaatgggaat aaaagtttac agagcagaag gtgatactat aagtgctaat ttagacttac
      781 ttaaaaatgg aaaattagta gagtttccat cagaccatac ttgctcacat gacggatgtg
      841 gacatcatta aaaaaaacag ctgatgaaat atatacttca tcagctattt tttcttttaa
      901 taaatacgat tttattcttt taaaaccaaa atttagattt ttatgttttt tgtagtatcc
      961 ttaataatat ttcaagttta caagtttact acttttagat tttttttgta tgtttttcta
     1021 tatagtcaat atcatttaat atgtcattga gttctttatt aagtgttttt aaatcatcac
     1081 catctacatt gtagtttaaa aatagttcct ctacattatc aatgatagaa ttgacttttc
     1141 tagtaacttc aacaaatgtt ttaaagttac tactattttt agcatttaga aattttttta
     1201 aatcctttga tataaatgat actatttcat attcatcatt aaatagacct ctaccaatag
     1261 gatatggttc aattctctgt aaaaaatatt ggtctttagc attaatatta taagtataag
     1321 atatttccca accatctcct aaatcaaact tgataaaata taatgtacta cttacttttg
     1381 atattcttgt ggcaccaagt tgtcttaaaa gaacttcaat cgaatcacct tgaacagtgt
     1441 taatattcat atatacacat cccctttagt attattatac tatatttttt aaaattttat
     1501 aattaaataa acagtagata taataataga tataatcata agtggaaaac ctattttgaa
     1561 atactcctta aaagagatag gatgaccgtg tttattacct atacctgcta gtacaacatt
     1621 agcagatgct cctattagtg ttccatttcc tccaagacaa gctccaagag atgttgccca
     1681 ccatagtggc attacatcaa taccttcagc ttgcatagtc aaaataagag gaattagtgt
     1741 tgcaacaaat ggaatattat ctaagaaaga agagactata gctgataacc ataatatcaa
     1801 caacattgta aatacaagat gtccttctgt aagtccaatc aatgcctgag ccaatttgtt
     1861 tattatccca acttctacta atccaccaac tactacaaat aatcccataa agaataaaat
     1921 tgtagaccat tcaatacctg ccataatttc atctacatct tgttttccta ttaaaagcat
     1981 tatagatgct cctgtcagtg ctacaacaga agaatctatg tggattgtac tgtgaaacat
     2041 aaaaccaaaa agtatgaatg ctattaatat aaggcttttt ataagtagag gtttatcttt
     2101 aacagatttt ttttcatcta gttttaatat tgcattttta gctctttcat tcacaacaag
     2161 ttcttttcca taaataaatc taaagcatat tatagttata gcaagtatta ctataacagc
     2221 aggacctaaa tttattacaa aatccataaa actaagattg gcagcactac caatcataat
     2281 atttggaggg tctcctataa gagtagctgt tcctcctata tttgaagcta aaatctgtgt
     2341 tattaaaaat ggaactggat taagtcctaa tatttgtgta ataactatag tcattggtcc
     2401 aataagtaag acggttgtaa cattatctaa tacagctgat aaaatagctg ttataatagc
     2461 aaagcaaatc attatcttcc atgggtcacc tttagctttc tttgcagtcc agatagctat
     2521 atattcaaat aaccccgagt ttttgacaac agctacaaat agcatcatac caactaatac
     2581 accaatagta ttgaaatcta tatggcttaa tccttcatca agtgtaagta tattgaatat
     2641 tatcattaat agtgcaccac caacagctgc tacggatctg tttacctttt ctgtaataat
     2701 agctgccata actattaaaa atatacatat agctattatt tgttgaaatt ccataatata
     2761 agtacctcca tttgtattat tttgtgctta atgctagtac tatatatagt attacttttc
     2821 tattgttttt tcaatggatg tatgaaagta tattatttaa caaagttttt gaaaaattga
     2881 cagaatttaa agaaaatttt tgttatttaa ataaatttta aaattattcc attgataata
     2941 atattaa
//
LOCUS       DACXMZ010000319         2944 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.320, whole genome shotgun sequence.
ACCESSION   DACXMZ010000319 DACXMZ010000000
VERSION     DACXMZ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.320"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            373..1650
                     /gene="hflX"
                     /locus_tag="JD016_18505"
     CDS             373..1650
                     /gene="hflX"
                     /locus_tag="JD016_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAU5001611.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1883..2380
                     /locus_tag="JD016_18510"
     CDS             1883..2380
                     /locus_tag="JD016_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAU5001612.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIEIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2430..>2944
                     /locus_tag="JD016_18515"
     CDS             2430..>2944
                     /locus_tag="JD016_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAU5001613.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYI"
BASE COUNT         1224 a          260 c          523 g          937 t
ORIGIN      
        1 taaataacta cttaaattta agaatagggt taaatataaa atgataatta catataagtt
       61 aattaaacta atgtattttt aatatatatt tagaatttta atccttctta tgatataagt
      121 agcttgtttt gtattactta gatttgtaag gagttttttt attgaaaaaa ctgcataata
      181 tatttgggag gaggtgtaag aaagtgattt tttatataag attgagaaaa taatgcacga
      241 ttatggttat tataatagta aataagaagt gtcatttaag gataagataa aaagcaaaat
      301 tcttgatgaa tttaattata gagccataat ctcgtgctat agattaaata aaaaatagca
      361 gataaggaga atatggatat ggcgaaaaaa ggaataacag taggattaaa cataaataat
      421 aaatctgagg actttaatga actaatgatt gaacttgaaa acttatgttc agcatgtgat
      481 attgatgttg tagggagtat aactcaaaat gcaaagcagg ttaatagagc attttacatt
      541 gggacaggta aagttgagga aatattaaac ttaataaaga aagaaaatat agagatagtt
      601 atattttaca atgaactttc tacatcacaa ctaaaaaatc ttgaagaaaa gcttaattgt
      661 gaaattatag atagaacggc attgatactt gatatatttg cacaaagggc aaaaactaga
      721 gaagctaaac ttcaagtaga agtagcaagt ttaaaatata tgttaccaag acttataggt
      781 tcaaatgaaa atttaggacg tcaaagtggt ggagttggta ctaaaaatag aggttctggt
      841 gagaaaaaat tagaacttga ccgtagaaga atagaagaaa agataacatc tttaaatagg
      901 gagttggatg atttaaaatt ccaaagagaa actcaaagaa gtatgagaag aaaatcaaat
      961 cttccaaatg tagctctagt cgggtataca aatgctggta aatcttcaat aatgaataaa
     1021 ctagtagata tatttaaaaa ctctgaagaa aagaaagtgt ttgagaaaaa catgttattt
     1081 gctacacttg aaacttctgt cagaaatata gttcttgcaa ataacaaaga atttttatta
     1141 tctgatactg ttggttttgt aagtaatcta ccacatgact tagtaaaagc atttaggtca
     1201 acattagaag aagcatgtga ggcagacgta ctgttacatg ttattgatat ttcaaatcca
     1261 agctataaaa gtcatattaa agtgacagaa gatactttaa aacaaatagg tgcagatggt
     1321 atacctatga tacatgtgta taataaaatt gatttaatag atgtagaagt attggataga
     1381 attttagata gtatcgataa agaaggtata tttgtttcag ttaaaaaaga tataaatata
     1441 gataaaatga taaaatgtat ttgtgatagc atatttaaag attatgtaag atgtaagttc
     1501 ttaattccat atgataaagg tcatgtagtt tcttatttta acgaaaatac gagcattata
     1561 aatacagaat acagagaaga tggagctatt ttagatgtag aatgtagcaa tatagaatat
     1621 aataagtata aaaagtatgc tttagagtaa attttgaaaa acttattata gcctaaaata
     1681 tatttaattt ataatttatt ataaattgat tgtaaatatg attttatgaa acggtatagc
     1741 cataatgcta tgccgttttg ccatttataa taaaaataaa tttattgaca taatagtaac
     1801 taattactat aatataaagt aattagttac tatttaaaat tatcaaaaaa ttaaaatcta
     1861 agataaagag aagtgtataa taatggatgc agaaagttta atgaaaaaat atatggtaga
     1921 tgatgatagt agaaaacaag ctatatttgc aagtatattt gtaatgcaaa ataaacttca
     1981 aactacatgt gataaattag acccagaatt aacaatgaaa caatggttac ttttagctat
     2041 tgcggactct gtagatgaaa ctcttacatt aaccaaactt ggagaattaa tggggtgttc
     2101 aagacaaaat gtaaaaaaac tagctattgc tttagaaaag aaaggtttta tagaaattga
     2161 gcaaaatgaa aaagattcaa gagctatctg tcttgtgaca aaagatagag tgcaagagta
     2221 ttctaaaaaa gtaagaggta tgcaggaaaa agtattacag cttttatttg aagattttac
     2281 agagaaagaa gtggaacagt tattttttgg aattatgaaa ttaagtatag gaattgaaaa
     2341 tgtgaaaaat tatgtagaaa ataatgacgt tggagaataa gcaagtatca aatcaacttt
     2401 gtatgacatc agagaggggg gtaatttata tgaaaaatgt agtagtgatt tataagagta
     2461 agtatggttc aacaaaaaaa tatgctaaat ggctttcaga attattgtct tgtgatattt
     2521 ttgaaactaa gaatattaat atagaaaagg ttatgaagta cgatacgatt attttaggag
     2581 gaggtcttta tgcttgtaat atagcgggaa tttctttctt aaaaaaaaat tataaagaat
     2641 tgcagaaaaa aaatttagta gtatttggcg taggagctgc tccatatgat gatgaagtaa
     2701 taaagaaatt aagagaagta aactttaagg atgaattaaa caaaattcct tgcttttatt
     2761 gtcgtggggc atgggatgag acaaaaatgt cttttataga ttatactctt tgtagttttt
     2821 ttaaaaaatc tatagctaaa aaagaacgtc aaggtcttaa accagcagaa gcacctttat
     2881 tgtctggaga agcctttgat tggtgtaata aagaaaattt aactccaata cttgattata
     2941 taag
//
LOCUS       DACXMZ010000320         2935 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.321, whole genome shotgun sequence.
ACCESSION   DACXMZ010000320 DACXMZ010000000
VERSION     DACXMZ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.321"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            468..1529
                     /locus_tag="JD016_18520"
     CDS             468..1529
                     /locus_tag="JD016_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAU5001614.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(1645..2619)
                     /locus_tag="JD016_18525"
     CDS             complement(1645..2619)
                     /locus_tag="JD016_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001615.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
BASE COUNT         1106 a          408 c          447 g          974 t
ORIGIN      
        1 tttttaatca aattttaagt tcaactcctt ttttatattt ttatagatat atcctatatt
       61 ttcccagaaa acgtattgaa tatacaaatt ataagaagta taatgttgtt gggagagaat
      121 gtatgaaatg tatacaaagt tgattttaac tgtattattg ataattctct ttactcttac
      181 cattattaat gattgaagac aatggtattt attataggtc aaagattccg aatgtattaa
      241 tcatcatcat tttctcaaac taacagcaaa caccctgatt tgcgagttgt attgtataca
      301 aattatctag aaaatatcta ttagattttc catgaaattt aaatatttca cgaatgtatg
      361 aatggagaaa tatacatgtt gacaatccac ctttttaaat cctaaattaa ttttattaat
      421 ggtagagaat aggaagtaac aatgttttat aaaggagaat attaagtatg aagaaaatta
      481 ggaagttagc taccaagtta ttgattacaa caatagtttt aatctcaggt atgagtatga
      541 cagtttatgg tatgacagcc aaagaggtaa cagcaaaaac accaaaaagc tatgtcactg
      601 gaaccaatag cgtatatggt ccaaagctgt cacaagccca attaaactca gtagcacaag
      661 caacagcaga ttttatgaac aagaaaatca caaagaatat gaccacagat gctaagattt
      721 tagttgccta caatcacatt aaaaataaca caacatatgt tgactggaat gcagtagagg
      781 gagcaaatac tgcctataca ttggttacta aaaaaggtgc atgctcagga atggctagga
      841 gcatgaaagc tttgtgtgat gctatgggaa ttgaatctta ttatgtacac agcacatcaa
      901 atgaccacca gtggaatctt atccgttttg gtgatgggcg tttgtatcat gtagatattg
      961 atgctaataa atcagcagga aaggatatca tatacaagag tcttagccac ccattaccat
     1021 ttgataagac agcatatcca aaagttggtg caatagtaca gacagtaaaa ccagaacaga
     1081 cacagaaacc agcaccacct gggaagcaca ctatgttacc acatcaagaa ggggcaaagg
     1141 ctgttggaaa ttttggaaaa aaaggagaag atataaatga aaaacatgta atgtatgaag
     1201 gtgttcgatg tagtgtagtt aattcatcag taaatacaat tacatttaca ggtagtgaat
     1261 caaatttaga cccatcaaat cttacgattg ctgtaatagg atttatgaac acaccatatg
     1321 ggatgtttgc attagcacca gagggtaaac ctgttgaata tccttttaaa tatgataagc
     1381 cattttcttt agattttagt ggtaaaaatc gttctggtgc agaattagcg aagtactgta
     1441 aaaaaaatga tgtgactatt aaaatagaaa tcactgatga aaaagctaat attggacttg
     1501 gtcaaataat gtatgttcga tatgaataat gatttaatca tgacagtaga ataagttata
     1561 aacattgcgc cctcttacta ggggcgcatt taatattata tctgatattt tgtagtcagg
     1621 tcaactgcta gatttctccc ctttttattt ataaaaatta ttatctctaa gcatctgagc
     1681 atgtttacga ttaactaaaa actgttttaa ctcagtcaag cataggaata aaatagctct
     1741 attttcaaac tctgttctgt caacaggtag aggctggttt ttcatactaa gcattagtct
     1801 ctttaattct tcaagaagaa gattgcctgt ttcagcttca aattcagtat aacctatctt
     1861 atgtataaaa gcagatagaa tatgagcttg tggtggtgta gaatttagtt gagatacata
     1921 actatacaaa ttttctaaga tatcccgttg actttttata atatccatat gctctaagaa
     1981 gaatctagta tcacttaata aagtattatt aatattatcg taagtttcag aaatactgct
     2041 gtcaataagt gaatttaatg tatataaatc tctactatat ccattttctt ttttaggatt
     2101 tacaataata tctgcaatgt ctataagtac aatactcact tcttcctgta actttttttg
     2161 aacttcatgg attttatgta tatttgttgg aatatacata tttattaata cacctattcc
     2221 agttcctact gcaaatattc cagcttcatt taaaatccat ttgatagaag tttctccttg
     2281 aaggtagtaa tgggatgcta tgactacaca catagcaata acatcttgaa tatttaacaa
     2341 aaaacaaagt ggtataatta taaatataaa tattgaaaat gaagtcaaat tatatcctaa
     2401 aatattaaaa cataaatatg aaaaaatagt acataaaaga aaaccataaa tctttccaat
     2461 ggagccttta agagtttctc ttttagtgtc ctttactgta agtagtacta taactccagc
     2521 aaccattgaa tattctaatt ttaatagcca agcaataaat atagcaagag aacatccaag
     2581 agataatttt attactttaa gtgaattaat attattcaaa tgcaaacctc ctgtaaatca
     2641 atataataaa attctaacat aaaactaata ttgcttgaaa ggtaagtaac tatacttaat
     2701 tattttattg ccctatttgt ttagatacaa tcaatataat tttattttat gaaaattttt
     2761 tgtaaaattt taaatttaga gactaacatg agaccacaaa gaggaagttt attataggaa
     2821 gaaaacttaa aattgatttt aagcagcaaa tgaaaataat caaatatgta tatactatta
     2881 ttaaaaaata aaattatata gttaatatga ttttataatt ggtttagtat agaga
//
LOCUS       DACXMZ010000321         2867 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.322, whole genome shotgun sequence.
ACCESSION   DACXMZ010000321 DACXMZ010000000
VERSION     DACXMZ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.322"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            81..1190
                     /locus_tag="JD016_18530"
     CDS             81..1190
                     /locus_tag="JD016_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAU5001616.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1567..2751)
                     /locus_tag="JD016_18535"
     CDS             complement(1567..2751)
                     /locus_tag="JD016_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001617.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1095 a          438 c          404 g          930 t
ORIGIN      
        1 atttactaaa aatacttaca aatatgatat tattaattgg ttaataaaaa agaaaatttt
       61 tcatgaaagg taagataaca atggatttca aaagcttagg gattagtgaa aatacaataa
      121 atattctaaa aaaatcagga ataacaacac ctacacctat acaaaaagaa agtattaaac
      181 taataaaaga aggaaaagat gtaatagcag aagctcaaac tggaacagga aaaacactag
      241 cttttttact tccgatattt gaaaatatat cattagatat aaatgatata caagtactta
      301 tactttctcc tacaagagaa ctagctattc aaataacaga agaagctatg aaacttaaag
      361 aatctaaaga tgtaagtatt cttgcagcat atggaggaaa agatattggt tctcaaataa
      421 agaaactaaa aggaaatatt catatgatta tagctacacc aggtaggctt ttagaccatc
      481 taaatagaaa aacaatagac cttagtaagt tgaagacttt tgttttagat gaagcagacc
      541 aaatgttgct tatgggattt aaaaatgaag tggaagctat tttaaaagag acttcaaaca
      601 aaaagcaaac actatgcttt tctgcaacta taaattctca agtaaaaaaa ttagcttata
      661 gatacacaaa aaatcctgtt gtagtttcta ttcaaaaaga agaaatcact cttaataata
      721 taaaacaaga agtagtagaa actacagata gaaaaaaatt agatgcctta tgtaaggtac
      781 tagatgaaga taatcctttt atggcaataa tattttgtag aactaagaga agggttgaca
      841 atcttgaaga agctcttgct ataagaggat ataattgtca aaaacttcat agtgatattg
      901 ctcaatcaaa acgagaaaga ataatgaaat catttagaaa tttagatata caatacttaa
      961 tagctacaga tgtagcttct cgtgggcttg atatatctgg agtaagtcat atatataatt
     1021 acgatttacc agaaacacca gaagactaca tccaccgtat tggtagaact ggtagagctg
     1081 gtgaagaagg ctatacatgt gcttttattg accctaaaaa tgaacgaatg ttaagtgaaa
     1141 tagagacggc tatagaatct aaaattagta gaagaattat agaattataa tttatagatt
     1201 gcttaaaata ccaatgccac acaaacactg taatatatag aaagctacag aaaacatgta
     1261 tcaatctata ctttgctgtt atactatttt atattaaaga aaaaagaggc tatccaaagt
     1321 aggattagtc tcttttattg tcaaaataat gaaaatatat ttgacttcat ataaaaaaga
     1381 gtaccttata aactaaaagt ccattttgag acaccctctt tttatctttt attcttagtt
     1441 ttatattgct tgtttatagt tgtttaatat tagatttttc aaataatctt aataaacatg
     1501 cagatatatt atctatagta ttcttattta tagtattctc tttagcaata ttttttgcta
     1561 ctacatctaa tttacatttt catttaattc cgctggccat ttagcttctg gattttcctt
     1621 tgcaaacttc ttattcttat aatgtcctag tgtaaggaat ggaattacta tagtgaatag
     1681 accaagataa ccacaatatc catatccata tttaacaata ttacttaatc ctgtaagtga
     1741 tataagcatt gacactacca ttattgcaca agctgtaaat acccttcttt tcattcttga
     1801 agaaaggaaa gaaagtgctt tatggtcttc aaatctattt acaagaccaa atatagtagt
     1861 tactcctgta gacattaaac aaaggaataa tgcaacatta taaattatgt atatagtatg
     1921 atttccagat tgacctgcaa cataaagagt agggagtgtt gtttgcccag cagcaataat
     1981 ttctctatac catcccatta gcatagttac agcaagtcct aatgcaattg cattcataac
     2041 aaatgaaatt atcattgcct tagtagcttc ttttgagttc ttaagaagtg gtcttgaaca
     2101 tcctgcaagt gtaggtataa caaccgattg aaaacctgca taaataaaag tattaagtat
     2161 tggcttgata tagccaggtg tgaaactcgt tctatttgat aatatttcag ttatttctgg
     2221 tactttagct tttataccaa caataaaaat agtaagagta catactagaa tagctattgt
     2281 cattgctgta gctgctttag atacaagcat agcaccaaat atagtaaaga caagaagtac
     2341 aactccaata ataaatacag ctacaaaata atttactcct atactttgaa atactgctgc
     2401 tgcacctgct ataacagcac ttacagctat taaaaccatt agatagtaat atatctccca
     2461 tattatttca agttttggat atggtctcca aagctcacaa aataaatcct tataattcct
     2521 acaattgtta ttattataca ttatgataac ttctctcatg gtagcagtaa gtataatcat
     2581 tgctaaaatt gccataagcg gtgctgtcca tccatactgc acatagtact gagtttcttg
     2641 attccctgta gcaaatcctc ctcctgcatg tgaactaaaa agaacgcttg caacaccaaa
     2701 aatggctaac atagagcccg ttacttttgc tttctttaca cttttttgca tgaaatcatc
     2761 ctccctaaaa tgatattgaa agtttttatt ttaacacttt attgataaaa caataacatt
     2821 tttgctcatt aaattcaact atatttttcc aatgacttta cattttc
//
LOCUS       DACXMZ010000322         2864 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.323, whole genome shotgun sequence.
ACCESSION   DACXMZ010000322 DACXMZ010000000
VERSION     DACXMZ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.323"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            82..2439
                     /locus_tag="JD016_18540"
     CDS             82..2439
                     /locus_tag="JD016_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAU5001618.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2525..2842)
                     /locus_tag="JD016_18545"
     CDS             complement(2525..2842)
                     /locus_tag="JD016_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAU5001619.1"
                     /translation="MSEFKNVTAAKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1073 a          324 c          474 g          993 t
ORIGIN      
        1 aataaaataa aaaaattgag aattttataa tatatttgta gatttgagta taaaaaagtg
       61 ctaagttgaa aggagtaaaa tatggataag gttaatttag aaaggtatag ttgtgatata
      121 aatgttggtt taaataaaaa tcaacttcaa agtagaatag atgataattt aattaatgca
      181 tttgataatg gaaaaaccaa aacatataaa caaattttta aagacaatat atttacactt
      241 tttaatctca taaatattgt tttagtctgt ttattagttt cagttggttc ttttaaaaat
      301 accttattta tatttattgc tgtaattaat actattatag gagtaataca agagataaga
      361 gctaaaagat tattggacaa tctatctgtt atagtttcaa ataaggtatc tgtcataaga
      421 gaaaaagaga aaaaagagat tgatgtacaa gaattagtat tagatgatat tatgatttta
      481 aaaacaggta atcaaatttg tgcagattca aaagttttaa atggaaaatt agaggtaaat
      541 gagtctttgg ttacaggtga atctgatatt attataaaaa ataaaggcga ttttttatat
      601 tctggaagtt ttgtagtttc tggagaagct tttgtaagag ttgaaaatat aggaaaagat
      661 aactatgcaa ataagatagc taatgatgca aagatttcta aaaaacataa ttctcaactt
      721 agaaattcat taaatgcaat attaaaaatt gtaggtataa taataattcc tttaggaatt
      781 atattgtttg caaaacaaca ttttggaaat ggagattcta tagctacatc tgtagtaggt
      841 acagtggcag ctgtaaatgg tatgatacca gagggattaa cattacttac gagcattgct
      901 ctagctgtag gaactataaa acttgctagt cataaaactt tagtgcaaga actgtactgt
      961 gtagagactc ttgctagagt tgatacacta tgtcttgata agacaggtac aattacagaa
     1021 ggtaaaatgc aagttgatga tatagtaatg ttggaagaag tggatattaa tgaaataatg
     1081 gggaatatat gtaattctct caaagatgat aatgcaacac taaatgcaat aagagataag
     1141 tataatgttt tagatacgta taaagctaag aatttaatac cattttcttc agaaagaaaa
     1201 tacagtgggg ttacctttga aaataaaggt acatatttat taggtgcatt tgaatttata
     1261 tttaaggaaa aaaataaaaa acttaggata gaagttgaaa gatattctaa aaaaggaaac
     1321 agagtaatag ttttagctca tagtgattct tatatggaag ataataatat tcctgaagat
     1381 ataaagccac tagcttttat attgatattg gataaaatta gagatgaagc taaggaaact
     1441 ttagaatttt tttacaatga aggtgtagat ataaaaatta tatctggtga caatcctata
     1501 acagtaagtg aggtagctag aaaagcaggt cttaaaacag ctagtaattt tattgatgca
     1561 acaacactta aaaatgatag tgatatatac aaagcagttg ataaatacag tgtttttgga
     1621 agggtaagcc cacaacagaa aaaacaaatg attttagcat taaaaaaaca aaatcataca
     1681 gtagctatga ctggagatgg tgtaaatgat gtgctagcac ttaaagaagc ggattgtagc
     1741 attgccatgg catctggaag tgatgttact aagaatgtgt ctaatttagt cttattagat
     1801 tcaaactttg catcaatgcc aaaggttgtt atggagggaa gaaaagtaat taataacatt
     1861 caaagagcat cttctttgtt tttagtaaag acaatttttt cattcttctt gtcgttatta
     1921 acattattct tcttcagtag acagtatcca tttgttccta tacaattatc actcattggg
     1981 gctgtaacag tgggtatacc atcattcttc ttagctcttg aagcaaacaa aagtagaatt
     2041 tcaggaaatt ttttactcaa tatattaaaa aatgctttac caggagcact atgtgtgaca
     2101 gtaaatgtaa ttattgttta caatatagtt gaatatttag gttatagttc aagtgtattt
     2161 tccacaatgt gtgctatatt aactggtgta tgtggtcttt taatattaag acaacaatgt
     2221 ttgccattta ataaagaaag attattttta atagtaagta tgactattgc atttgttatt
     2281 gggattctat ttttaggtgg attattctca tttgtagcat tgagtagaga attaattctc
     2341 ataactatgg cattagcaat attaatgcca attatgataa aagttatgtt atttgtctta
     2401 gatgagatat tagagacaat tgttccagat ttaaattaat tttaaaatga tataaataag
     2461 agcagtttta gttgaactgc tctttttaga tgtgtttaat tttatgtaag aatatgtatt
     2521 tttattaatc agctatatat gagcaacagt agtcaacaac ttcattaacc tttaagtcaa
     2581 aacttgaatc actaggaaca ttaaattttt gcccttcttt ataagttaca aactcattgc
     2641 ttccaggcag ttttacatcc atacttccag caagcatctc cattatttct tcttctctag
     2701 tagaaaaagt gtattctcct ggcaacatta atcctaaagt ttttctttct ccattaggca
     2761 gtattattac tctgctactt accttaccat caaaataaac gtttgccttc tttgctgcag
     2821 taacattctt aaattcactc attagataaa acctccaaaa ttat
//
LOCUS       DACXMZ010000323         2861 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.324, whole genome shotgun sequence.
ACCESSION   DACXMZ010000323 DACXMZ010000000
VERSION     DACXMZ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.324"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..387
                     /locus_tag="JD016_18550"
     CDS             <1..387
                     /locus_tag="JD016_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAU5001620.1"
                     /translation="TGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEYLKI
                     SGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDELER
                     LKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            377..1870
                     /locus_tag="JD016_18555"
     CDS             377..1870
                     /locus_tag="JD016_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAU5001621.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSIKYYLELEKQYKLAMSNIERDI
                     LIWYNRFTENEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKVISKPWTSDGLNFSERIWGKHRPALVNELHTKLTQSIIRGENPKKLV
                     NDFAKRFKVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKYVKNNSKQTGAKYTKGDIEWNIRREEEAELYYDNIRNRKDDISKISKNTNWSE
                     KSIGQIKNHIFYNTHIMRDGTRRMLDSDYSMSVAWQRLINGTYEDIDILLLKHEYLES
                     IFEKKYNISNLEAHRMTEKKHDWYKELIKQKGEFEEDDCLNELIRKE"
     gene            1848..2078
                     /locus_tag="JD016_18560"
     CDS             1848..2078
                     /locus_tag="JD016_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001622.1"
                     /translation="MNLLEKNNEYVIYSYGYEENKLDGRIKIYLDDFYNYEIIKESKD
                     EHISKSATLKAISKLIKAAKNNDLKKEMSYQC"
     gene            2388..>2861
                     /locus_tag="JD016_18565"
     CDS             2388..>2861
                     /locus_tag="JD016_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HAU5001623.1"
                     /translation="MDWLEELLEGIKIENNKIDVASLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDMEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKI"
BASE COUNT         1231 a          276 c          528 g          826 t
ORIGIN      
        1 acaggtgaca aatcgggtgt agcacttaaa tttttatatt cactacttga cttaaaatgt
       61 tctaagactg aaaagaagtt taaaaaagca attagagagc ttttatggtt tgtgtgtgag
      121 tatttaaaga taagtggtag taagagctat gattataaaa cagttcaaat tacttttaat
      181 cactctatga taataaatga agctgaaaag atagatatgg cagctaaatc aactggaatt
      241 gtatcagatg aaactattgt ttctaaccat ccttgggtcg aggatgttaa tgacgaactt
      301 gaaagactta aaaaacagga agatactcaa aaagagtatg atgatttaat tcctaataat
      361 caagatggtg ttatagatga aacataaaga ttattggagg aagagatttg aacaattaga
      421 agaagctcag aataataaaa gtataaaata ttatcttgaa ttagaaaaac aatataaact
      481 agcaatgtct aatatagaaa gagatatact tatatggtat aacagattca ctgaaaatga
      541 gggaatatct ttattggaag ctaagaaact gctaaataca agagaactag aagagtttaa
      601 atggagtgta gaagaatata ttaaatatgg taaagaaaat gctataaatc aaaagtggat
      661 gaaagagtta gaaaatgcta gtgcaagagt tcatataaca aggcttgaag ctttaaaact
      721 gcaaatacag caacaagtag aagttttata tggaaatgaa cttgatggta ttgataaact
      781 aatgagagat atttatacaa gtggatacta tcatacagct tttaatgttc aacaaggagt
      841 aaacgttggt tggagtttaa tgagtcttga tactaacaga ataaataaag ttatttctaa
      901 gccttggact agtgatggat taaacttcag tgaaaggatt tggggtaagc atagacctgc
      961 tttagtaaat gaattacata ctaagctaac tcaatcaatt attagaggtg aaaatccaaa
     1021 gaagctagta aatgactttg ctaagagatt taaggtatct aagtcacaag ctaagaactt
     1081 aataatgact gaatcagctt tctttgcatc agcttcaaga aaagattgtt ttaatgattt
     1141 agatgtagag aaatatgaga ttattgctac attagattta agaacttcaa atatatgcag
     1201 agagttagat ggaaaaatat ttgatatgaa agattatcaa gttggaataa cagctccacc
     1261 atttcattgt cgttgtagga caacaacagc tccttggttc gaggatgaag aaggctatag
     1321 agcagcaaga ggagaagatg gaaaaacata ttatgtacca tctagtatga agtataatga
     1381 gtggtatgag aagtatgtta aaaataatag taaacaaact ggtgcaaaat atactaaagg
     1441 tgatatcgag tggaatataa gaagagaaga agaagcagaa ctatattacg ataatattag
     1501 aaatagaaaa gatgatattt ccaaaatatc aaagaataca aattggtcag aaaaaagtat
     1561 aggtcaaatt aaaaatcata ttttctacaa tactcatata atgagagatg gaactagacg
     1621 tatgttggat tctgactata gtatgtcagt tgcttggcaa agacttataa atggtacata
     1681 cgaagatatt gatattctct tattaaaaca tgaatacctt gaaagtatat ttgagaaaaa
     1741 gtataatata agtaacttag aagcccatag aatgactgag aaaaagcatg attggtataa
     1801 agaattaatt aaacagaaag gagagtttga agaagatgat tgtcttaatg aacttattag
     1861 aaaagaataa tgaatatgtt atatatagtt atggatatga agaaaataag cttgatggaa
     1921 gaataaaaat atatttagat gatttttata attatgaaat aataaaagag tcaaaagatg
     1981 aacatataag taaatcagca acgttaaaag ctatttctaa acttataaaa gctgctaaaa
     2041 ataacgattt gaaaaaagaa atgagttatc aatgttagaa gcacttactg aacaataaat
     2101 tagtaggtgc ttttattatg taaaagttta aaaaagtagg taattttaat gtaaatatta
     2161 actcaagtta tagctgtagt ttgtgtagta caaatcttta ttaattgtat tgctaatgtc
     2221 aatgtaggta ttctttgcaa taaattaaaa gaaaaaaatg aagctaatat agataaagtt
     2281 tctgatgaaa ttctaaagag agtaggagaa gaattaaata aatcactaga caaaagtctt
     2341 taaagacttt ttttattgtg taaaaaatga aaggagatat ttaaaagatg gattggttag
     2401 aagaattgct agaaggaata aaaatagaaa ataacaaaat tgatgtagct tctttacaaa
     2461 agtctataga aaagaaaata aaagagacta caattactca agaagattat acaaatcttg
     2521 aaacacagct taatacagct aatgaaacta ttaaaaagtt tgaaggaggt atgacaaaag
     2581 aagatgtaga gaatctaaaa acaacttatg aaactgataa gaaaactttg gaagaaacct
     2641 acaaaaaaga aattgaagaa aaagacttta attactggtt aggtgatgct tttaagtctg
     2701 ttaaatgtag ggatgaaata gcattaaaag ctcatttaga catggaagca ctaagaaata
     2761 gtaaagatag acaaaaagct tttgaagagc aaataaaccc tttgaaacag gataaagatt
     2821 atttgtttaa tgcaacacta gaaggtgaag aacctaaaat a
//
LOCUS       DACXMZ010000324         2859 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.325, whole genome shotgun sequence.
ACCESSION   DACXMZ010000324 DACXMZ010000000
VERSION     DACXMZ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.325"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            158..1081
                     /locus_tag="JD016_18570"
     CDS             158..1081
                     /locus_tag="JD016_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HAU5001624.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1056..1883
                     /gene="panB"
                     /locus_tag="JD016_18575"
     CDS             1056..1883
                     /gene="panB"
                     /locus_tag="JD016_18575"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAU5001625.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1902..2750
                     /locus_tag="JD016_18580"
     CDS             1902..2750
                     /locus_tag="JD016_18580"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAU5001626.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PDNIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1148 a          334 c          463 g          914 t
ORIGIN      
        1 aaaatggtaa gatatttatg gatggtatct tgttataaga actatacttt aaatgttttt
       61 aattattttg tcttatgtaa ctcatatcct caggaatgag attagaaatt aagatgttgt
      121 atcacaaagt ggaggtgata ctattaaaat aggctttatt ggttctggaa aagttggaac
      181 atctttggca aattattttt tatcaaaagg ttattgtgtt tcaggctttt atggaaggaa
      241 tcaattatct ttattagaat caacaaattt aacaaaaaca aagatttaca gcaatttgaa
      301 agatataatt tatgaaaacg atatattatt tattacaaca tcagatgatt ctattgaaat
      361 tatagacaaa aaactttcca aatttaattt aaaacataaa tacatttgcc acacaagtgg
      421 ctctttaaaa tctagtatat tgttctgttc aaaaaaagca ggtgcattaa tttattctat
      481 ccatcctata tttgcatttt ccaataaaaa taccaacata aaaaaaatga aagacatttg
      541 tttttccata gaaggtgata atgaacaaga tgatttagtt attcaaaatt tcatagatga
      601 tttgggaaac caatttttta taagagataa ggatacttca tccacttatc atttagctaa
      661 tgttttagtt tcaaatttag tattatcttt actagatata ggagtaagtt actttattaa
      721 attaggttta tctgaagagg aatctttaaa agctattagt cctttagtaa aaaaaaatat
      781 agaaaatata cttgataatg gctttactaa agctcttact ggtcctgttt ctcgtggaga
      841 tataacacca ataaggaaac atctatctgt attggataaa aaagatgagg atatttacaa
      901 aatactatcc ttaaacctac taaaaataat tgccttagga aatgacaatt cattaaaagg
      961 tataaaaaat attactatag aaaatgcaat cgaaaattta ataagtaaat ctgaaaaata
     1021 caaagaaatt tacaaacttt taggaggaaa aaacgatgaa aaataccata cagactttta
     1081 agaatgctaa atatgaagga aaaaaactat ctatgcttac tgcttatgac tactcaattg
     1141 caaaaataat ggatgagtgt gatataaatg gtattttaat aggagattca ttaggaatgg
     1201 ttataaaagg tgaagaaaac actctatctg ttaccataga tgaaattata tatcatacaa
     1261 aagctgtaaa aaatggtgta aaaaatgcct taatcgtaag tgatatgcct tttctatctt
     1321 accatgtttc tatagaagat gcagttaaaa acgctggtag acttataaaa gaaggaggag
     1381 ctcatgctgt aaagttagaa ggtggctcaa acgttataaa gcaaatagaa agcattgtaa
     1441 atgctcaaat acctgtaatg gggcatttag gattaactcc tcaatcagta aattcatttg
     1501 gtgggtttaa ggttcaagga aataccagtg aaacagcaag acaacttatt gaagatgcta
     1561 aattaataga aaaagcaggt gcattctcta ttgtccttga aggagtgcct acgaaaatag
     1621 ctgagatggt aactaattct atttcaattc ctacaatagg tataggggca ggaattaact
     1681 gtgatggaca aattttagtt tatcaagata tgttgggaat gtttggagat tttgttccta
     1741 agtttgtaaa gcagtatgca aatataggag atataatgaa agattcaatt aaaaattaca
     1801 tcctagaagt taatacaggt gcatttccac aagaaaaaca cagcttttca ataaatgaat
     1861 ctgagttaga aaaattatac taatattaag gatggtacaa aatgcttgta aaagaaatta
     1921 aattactaag aaatataatc aaagattgga gaaaacatgg ctattctata gggcttgtta
     1981 caacaatggg atttttacat gaaggacacc aaagtcttat aaaaaaagct gtaaaagaaa
     2041 atgataaagt agtagtgagt gtatttgtaa atccaactca atttggacca aatgaagatt
     2101 ttaatagcta tccaagagat atagataaag attttaaata ttgtatggat agtggagcaa
     2161 cagtagtctt taacccatca ccagaagaaa tgtatttaaa aggtaattgt actaccatta
     2221 atgtttctgg acttacagat tttctttgtg gagctaaaag acctgttcat tttggaggtg
     2281 tctgcctagt tgtttcaaag tttctaaata tagtaacacc tgataaagct tattttggag
     2341 agaaggatgc ccaacaatta gctgtaatta aaagaatggt caaggattta aacatagata
     2401 cagaaataat aggctgtcct ataattcgtg aaaatgatgg attagcaaaa agttcgagaa
     2461 atacatatct ctctgaggaa gaacgaaaat ctgctctaat attaaataag agcttaagtt
     2521 tagcaaaaga agaattagta aaaggcaatc taaatccgga taatataaaa gaattaataa
     2581 cagctaaaat aaattctgaa cacttagcaa aaatagacta tgttgaaatt gtagatagtg
     2641 agacattaca acctgttaag caaatagaac attctatact tgtggcaatt gctgtgttta
     2701 taggaaaaac cagactaatt gataatttta catttgagtt aaatatatag tctataattt
     2761 tatataatat atctaaaaaa taataaattg cttagaaaat aatagattac aataactaaa
     2821 attttaaaat taaattcctt aaaagtatga ttaattata
//
LOCUS       DACXMZ010000325         2829 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.326, whole genome shotgun sequence.
ACCESSION   DACXMZ010000325 DACXMZ010000000
VERSION     DACXMZ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.326"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..254)
                     /locus_tag="JD016_18585"
     CDS             complement(<1..254)
                     /locus_tag="JD016_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5001627.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKIS"
     gene            complement(1063..2100)
                     /locus_tag="JD016_18590"
     CDS             complement(1063..2100)
                     /locus_tag="JD016_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAU5001628.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(2197..>2829)
                     /locus_tag="JD016_18595"
     CDS             complement(2197..>2829)
                     /locus_tag="JD016_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAU5001629.1"
                     /translation="GTGKSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIVSRV
                     FQDPSLGTCPSMTVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDI
                     KVQYLSGGQRQSLSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITT
                     LMVTHNLKHALQYGDRLIMLHKGEVVLDVKDKEKEDLTVEEILEKFEYAV"
BASE COUNT          954 a          434 c          366 g         1075 t
ORIGIN      
        1 ttagatatct taaacttaat gtgaagaatc cctgtaacaa gaccagcagt acaaccacat
       61 aagatagcca ttaaagttgc tactacagga gaaataccat tagtaagaga aaatgctaca
      121 atagaagcac ccattgtgta gctaccatca gcagacatat ctggaaaatc taagattttg
      181 taagttatat aaacccccag tgccataatc gataaaatta aactttgcgt cattacagat
      241 ataatacctg acataaataa aacactcctt tgaaaatatt tttataattt taattatttg
      301 ctaattttgt gtataaaaaa accacatagt gtacaaaact atgtggtaaa aacacgaata
      361 agccacatag tttaatctat gtggctctta tatagttaaa tgaaaacttt ataaacaata
      421 tagccaacat agatacaatc ccaaaatttt agtagtctta aggggtctgt atctatgtta
      481 aaacgtaggt acaaacccta aaaatagtgg ctataataat aataatattt tctattgttt
      541 aataaagtat tcataattat ctctcccttt taatatttta attagtcaga aaattatatg
      601 tattctcgac ttgttataaa ttataaatta tacaataata aaagtcaact gctaaattta
      661 attaaaatac taaataatgt atactcagaa tacttttaaa ttaatgtatt gtttgaaaaa
      721 aacgttatcc taagttataa gtgaatgtgg aaattttatc aaaatattta ctaaatatgg
      781 aataactaga catgcatatc aatcaaatat cataaaaaaa agctgtctgg agaatattaa
      841 aattcttttg gacagctcaa gtagtgatta gaaagttata taactttcat aatcagtcgt
      901 caatatagac gacttttatg gaaaatgaat aactttatat gcaatattca aattgctaca
      961 ataagcacaa ttttcaaagg gttagggtgg ataagagtac ttattatata aagttttcat
     1021 attacctact tcatattacc tattttttga aattaataag ttttattttg cagattttgc
     1081 attttcatct gactttatat actttatatt atctgctgat aacttttgca tgtcaagtgc
     1141 ttttagtgta tcttcgttaa ttattatttc agtttcatct aatgtagtaa ctggtatatc
     1201 gccaacagat tttccttcta atacttcaat agcaagttct ccagccttat aacctagttt
     1261 ttcataatct attccacaac aagctaaagc accagaagat acagaacctt cttcagaggc
     1321 tattatagga atcttgtttt cattagcaac tttagaaact attggcatag aagaaactat
     1381 taaattgtca gtagggacat ataaaacatc tattttgcca actaaactag aaatagcttg
     1441 gttaacttca cttgaagagc tgattccttt ttcaactact tcataattat ttttcttagc
     1501 ataatcatgt agagaatcaa cttggatttt tgaattaact tcactagtat tgtatataac
     1561 ccctattttc tttgcttttg gagtcaatgt tttaaccaat tctaatgttt tatcaattga
     1621 aagataatca gatgtaccag aaacatttcc acctggtttt tcaagagatt taacaagtcc
     1681 agcttctaca gggtctgtaa cagcagtcat tattatcgga atatctttag tagcattata
     1741 agctgcttgt gcagatggag tagatatagc atatatcaaa tcttttttgt cggatacaaa
     1801 cttactagca atactttgtg tagtaggcat atcattttgt gcattttgga aatctatttt
     1861 tatattatct ccatctttat agcctttatc ttcgagtgct ttgatgaatc ctttctttgc
     1921 tttatccaga gatggatgtt ccactaattg agtaatacct atattttttt gttttttgct
     1981 atctgtttct ttattttcat ttgaagcatt ggagccatca ttttgagaac atccagtaag
     2041 cattgataca ccaagtactc ctgctataat taaacttaat atttttttac tttttatcat
     2101 tttattttcc tcccttaaaa tttattaatt atatttgttc atatattcat atgatagatt
     2161 ataaatattt ctgattaaaa ctaagatttt aatttcttat actgcatatt caaatttttc
     2221 taagatttct tcaactgtta aatcttcttt ttctttatct tttacatcta atacaacttc
     2281 acccttatga agcattatca acctatctcc atattgaaga gcgtgtttca agttgtgagt
     2341 aaccataagt gtagttatat ttttctctct tacgatttta tctgttaact ctattacttc
     2401 atttgatgtt tttgggtcaa gtgctgctgt atgttcgtct agtaataaga ccttaggact
     2461 tgctaagcta gacataatta gtgatagtga ttgtctttga ccccctgaaa gatattgtac
     2521 tttaatatct aaatattttt ttaaatccaa agaaattcct tcaagtaagt gttccaagtc
     2581 attcgtttta tgacgaagac atttttttaa gttcaataat ttccctttat taagtgctag
     2641 agataaattt tctctaaccg tcatagaagg acaagttcca aggcttgggt cttgaaaaac
     2701 tctacttact atttgagttc ttttatactc agctaaattt gttatagtag tactatctaa
     2761 agtaatttga ccagaagatt cttttataag cccagatatt atatttaata aagtagattt
     2821 accagttcc
//
LOCUS       DACXMZ010000326         2752 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.327, whole genome shotgun sequence.
ACCESSION   DACXMZ010000326 DACXMZ010000000
VERSION     DACXMZ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.327"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            293..796
                     /locus_tag="JD016_18600"
     CDS             293..796
                     /locus_tag="JD016_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HAU5001630.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            843..1802
                     /gene="moaA"
                     /locus_tag="JD016_18605"
     CDS             843..1802
                     /gene="moaA"
                     /locus_tag="JD016_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAU5001631.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            1806..2273
                     /gene="moaC"
                     /locus_tag="JD016_18610"
     CDS             1806..2273
                     /gene="moaC"
                     /locus_tag="JD016_18610"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAU5001632.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKF"
     gene            2312..2743
                     /locus_tag="JD016_18615"
     CDS             2312..2743
                     /locus_tag="JD016_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HAU5001633.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         1144 a          252 c          536 g          820 t
ORIGIN      
        1 atttgtagag aaaaatatgg acaatgttat aataattata gaaattaaaa gtacttacat
       61 tcattatgta tgtgacatgt ataattagtt ttatatttaa tacagttttg aagtaatatc
      121 ttcattttga gaacgttgga ataataaaaa atgtattatt ataggtgata taaataaaaa
      181 ataaatataa tatgttatat tgctgataga aaagagaata aaagaaaata ggttttatta
      241 aaagagattt ttatttatag gacttaatca atgtatagag gggtgagaac atatgtttaa
      301 tgttgcaatt ataacactta gtgataaagg atatgaagga aaaagagaag atataacagg
      361 aaaaaagtta actgaatttg ttgaaaatac aggagcttat aaagttactg aatatgtact
      421 tataaaagat gataaggaaa tgctaaagga aaatattata agactttgta atagtaataa
      481 aatagattta attcttacaa atggaggtac tggtttttca aaaagagata taaccccaga
      541 agcaactaag gaagtgttgg aaaaagaaat accaggtctg agcgaatata tgagaatgaa
      601 atccacagaa attactaaaa aggccatact aagtaggggt gtaagtggaa taagaaataa
      661 ttctataata ataaatttac ctggaagtcc taaaggtgca gttgaaaatc taagttttat
      721 aattgatgtt ttagaccatg gcatagaagt tttaagagga gaagctacag aatgtgccac
      781 taaaaaaagt gaatagtata taatctagat agataaaaaa tagacaaaga ggtagataaa
      841 gtatgattga taagtatgga agaaaaatag actaccttcg tatttcactt acagataagt
      901 gtaatttaag gtgtgtttat tgtatgccac cagatgtaaa atttgataaa aattatataa
      961 atgaaaatct aagctttgaa gactacaagt ttattataaa ggctatggct gaacaaggta
     1021 taacaaaaat tagatttact ggaggagaac ctcttttata tcctaaatta aatgagctta
     1081 taaaatttac aaaagaagaa tgtggaataa ataacatagg aatgaccact aatgctattg
     1141 ggctttctga tagaatcttt gagcttgaaa aatgtggctt aaataaagtt aatataagtt
     1201 tagattcttt gaaggaatat aagtataaat ccgtaacaag aggaggaaac ttaaaagatg
     1261 tcttaaaatc gattgaatct tgtttaaact taggtataaa agtaaagtta aattgcgttg
     1321 ctataagtgg gtttaatgat gatgagctta aagattttat gtttttaaca gttaaatcac
     1381 caattgatgt aagatttata gagcttatgc cattaggtga ggctaataga gtttataaga
     1441 agggttattt taatgtaaaa gaaatgattg aaaaaatata tggtttatat aaagtcaaaa
     1501 atgatgaaaa aagtacagca gaatattata tgtttaaagg agctaaagga agaataggaa
     1561 ttataactcc attaagctgt tcattttgta acacttgtaa tcgtattaga cttacttcta
     1621 gtggtgcaat aaaattatgt ctacattcaa aagaagaaat agatataaaa ccatttttac
     1681 ataaaccatt gttatttaga gaatctatga aagatataat aaaacaaaaa ccagaaaaac
     1741 atcatttaat agaaaataaa tgtagtgata caaataggcg tatgtatgaa attggaggat
     1801 aagtaatgtt aacacatata aatgaaaaag ggtatgccaa gatggtggac ataagtgaaa
     1861 aagatgatac taaaagaacg gccgtagcta cagctacaat tactcttagt aaggaagcat
     1921 tagaaaaagt aaaaaatggt cagataaaaa aaggagacgt attgtctgta gctcaagttg
     1981 caggtataat gggagctaaa aatacatctt caaacatacc aatgtgccat cagattaaat
     2041 tagttggatg tgatttagaa tttgatgtag atgattctat gtctgctatt aagatatacg
     2101 caacctgtaa atctcttggg aaaactggag tagaaatgga agctttaaat gcatgttctt
     2161 tagcagcact tacaatttat gatatgtgta aagcagttga taaaaaaatg gttataagta
     2221 atatccatct tgtggagaag actggtggaa aatcaggaga ttttaagttc tagtaaatta
     2281 ataatatatt gggttaaaat ttggaggaaa tatggcgaag gtagtgtcaa taaatataag
     2341 tgagaaaaag ggtgtaatta aagttcctgt acaatgtgca gagctaaaaa taaatcatgg
     2401 gatagttgga gatgcacatg caggaaactg gcatcgtcag ataagcttac ttggaaaaga
     2461 aagcatagat aaaatgaaaa tgaaaggctt tgaccaatta aaatatggag attttgctga
     2521 aaatgtaact acagaaggga tagaagtttt ttccctgcct gttggtacaa agttaagaat
     2581 agggcaatgt gaagttgaag taactcaaat agggaaaaaa tgtcacaatg gatgtgaaat
     2641 taagaaattg acaggtgact gtgtgatgcc acgagaagga atttttgtaa aggttctaaa
     2701 agaaggaatt ataaatgtga atgattcaat agatgtaatc taatgaagtc ac
//
LOCUS       DACXMZ010000327         2746 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.328, whole genome shotgun sequence.
ACCESSION   DACXMZ010000327 DACXMZ010000000
VERSION     DACXMZ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.328"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            424..2235
                     /locus_tag="JD016_18620"
     CDS             424..2235
                     /locus_tag="JD016_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HAU5001634.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAVLSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQIENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVRKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2323..>2746)
                     /locus_tag="JD016_18625"
     CDS             complement(2323..>2746)
                     /locus_tag="JD016_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001635.1"
                     /translation="IPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVAT
                     EKTFINGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEY
                     VELTNDSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1046 a          280 c          404 g         1016 t
ORIGIN      
        1 caaaaaaaag gaatatattt acataatatg ataaaataaa ttcaaagagt taaattgtag
       61 gtaaatagta ttttaattat ttgttaaaat atagattatg tatatgccaa atttaaaaag
      121 agacataaag acattataca aatatattat tggggtgata ttttataaga aaagaagtga
      181 tgcagtaaat taattgagca atttaaaaat gatttaatga tagtgttgat gaatgtttaa
      241 aaagaaattt tagaaaaacg tatattgctg tttgtgttgg aggtgatgaa tttgtataat
      301 ttataggata taaatgatta aactatttaa gataaataca tatcactgta aaatattgat
      361 aatcattttt tataagagat tgattatttg caaattatat atattacaaa tgaggtaaca
      421 ataatgaaga aaaaatatga ttttttaatt tattctgcta tttggctagt tattattatt
      481 ctttttatat tttctctata tcatagtata gagcatatta aaattataag tcacactggt
      541 actattcgca gtgaaactca aaaggttgtt aagcaagaat tgaataatga aagaaatgat
      601 gacttgataa aacgactgga taatattctg ataaagctaa gaacaggtaa tggagaaaat
      661 ggatttcaaa gatgtgataa taaagaattt caacaaaaac taaatcaaat ggattctatg
      721 tgggaatcta tgaagaaaga aattataaaa gttagaaatg gtgcttcagg tgataaatta
      781 tataaattga gtgaagagta ttctgtgttg tctaatcaaa ttgtttttat atctgaaaaa
      841 cactctaatg caaaattgta ttcttttgct actgcactat ttatttatct tatattctca
      901 acaataagtc ttttaatttg ggaatattat aataaaaaaa ggtttaaaag aatattttat
      961 actgataatt taacaaaaat aaaaaatcag gttgcatttg aaaacagagc gattgaaatt
     1021 ttgcacaatg catctaacaa agagtatgta ctgctaaata ttgatattga caattttaaa
     1081 tatattaatg atacacatgg atatgaatat ggagataaag ttctaataat agttgcagct
     1141 gtactttcta aaacttttaa tatcaaagaa acttgtgcaa gaattggttc agataatttt
     1201 gtaatacttg caaaatatag agattctctt ttagaggata ttcgggaaat gctaactaat
     1261 gctataatct ctgaattaga tatgaatgta actcaaacta tatcttattg tataggagcg
     1321 tacttagttg aaattgataa tttaggttat aaatccatca attctataat ggataaggca
     1381 aatattgcac acaaggtaag taaaacaaga ggaatttcct ccacagtatg gtataatgaa
     1441 aatctgttaa aacagttaca aatagaaaat agtatttata attatatgta taaagcgata
     1501 gaaaatgaag aatttcatat gtatttgcaa cctaaatttc aaatatcttc tttaaatgtt
     1561 gtgagcgcag aagctcttgt aagatggttt tctccagaac ttggtttttt atcaccagat
     1621 gaatttattc cattgtttga aaaaagtgga tttattattg aattggactt ttatatgtta
     1681 aaaaaagcgt gttcatttgt aagaaaaacg tttatgaaaa aaaatcaata tacatatccg
     1741 attgcagtta atttttcaag ggttactatt taccaaaata gtttttatca aaggttttta
     1801 gatactgtta aagaatatga aattcctttt aagtatatag agatagaggt aactgaaagt
     1861 gcttttaatg aaatatctca accagttatt tcaattttag aggaacttaa aaagttagga
     1921 tttttaattt caatggatga ttttggttca ggatactctt cattaagtct tttatgttct
     1981 ctatctatta atggattgaa gttagataaa agcttactta aagaaacctt taaccgggag
     2041 aaagtatata gtattattca gtgtattatt gaaatgtcac atcgtatagg catgtctgtt
     2101 gtatgtgaag gtattgaaac taaaaaagac ttggaatttt taaatacagt taaatgtgat
     2161 gtaggacaag ggttttattt ttctaaaccg atagaagaaa aagaattttt taacaaatat
     2221 gtaactaaaa aataatataa ttatttttat cagtctatac attgagtcat tttatatctc
     2281 gttaaatgac tcaatgtact gttatgtatt ttaaaatata aattatttaa aacgtttttt
     2341 gtacttatat agaaattctt ctatattttt tgattgttct cttatttcaa ctgcttctat
     2401 atctagtaaa taaagtatag aatcatttgt aagttcaaca tattcaccat cttctgaacc
     2461 gagtgcatgt ctctcaattt ttaatccttt ttcttcataa tatttttttg tttctatata
     2521 tagtgattct cgcttttgta tgtagtcttt cacttcatta tttggaaagt tatatgaatc
     2581 cactaatgtt tcgccattta taaaagtttt ctcggtagca actttttcat cccaacaatg
     2641 gatttcttcc caagtaaaaa atatctcagt tccatctgga atgaatgaaa acctggtaat
     2701 aatatataaa taattattga aatttacttc ataatattca ggaata
//
LOCUS       DACXMZ010000328         2739 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.329, whole genome shotgun sequence.
ACCESSION   DACXMZ010000328 DACXMZ010000000
VERSION     DACXMZ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.329"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            110..1330
                     /locus_tag="JD016_18630"
     CDS             110..1330
                     /locus_tag="JD016_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAU5001636.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHISDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEKKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            1367..2716
                     /locus_tag="JD016_18635"
     CDS             1367..2716
                     /locus_tag="JD016_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAU5001637.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPIVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
BASE COUNT         1037 a          344 c          454 g          904 t
ORIGIN      
        1 tatatttatt caatattctt ataaaaaaca aaattttcca tcgaaaatta tggcataata
       61 attgcttatt atattaataa aacaataaaa agcaattgga ggaataaaaa tgcaacaggc
      121 tttgaaatta aaatatcaaa ccaaagaatt aggaaaagat tttgtaatag aaagttgtaa
      181 ttctataaaa ccatggctta taaacataag aagagaatta cataaaattc cagaattagc
      241 tcttgaagaa aatcttacta aacagaaggt aatttcttat ttaaaagaaa tcggaattga
      301 ttatatggaa tttacaaaac acaatggaat tatggcatat attttaaaag aaagtgcaga
      361 taaaacgatt tgtataagag cagatatgga tgctcttcct attgaagaag aaaacaacat
      421 accttataaa tcaatacatt ctggaaaaat gcatgcttgt ggccatgatg cacatacaac
      481 catgctactt ggagcatgta aagttttaca ttcaataaaa gataaactta atgtaaatgt
      541 aaaattctta tttcaaccag cagaagaagg ttttggagga gcaaaatttt tagttgagga
      601 tgggtgttta gaaaacccca aggccgatta tatattcgga cttcatgtta tgccacatat
      661 agaaacaggc cttatcgaaa caaaatatga tacattaaat gctagtgtag acactataaa
      721 aataagtatc aaaggtaaaa gagctcatgg tgcatatcct gaaaacggta ttgatgcaat
      781 agttacagct tcacaaattg ttacatcatt acaaactata ataagtagaa acttagaacc
      841 aaataatgct gctgttttga ctataggtaa aatatatggt ggtgatgcac acaatgtaat
      901 atgtgaagat gtaaaattag aaggcacttt aagaacctta aatagcaaaa caagaaactt
      961 tatgattgat aaaatagcta aaattgttgg agatactgca tctgcatttg gttgtgttgg
     1021 gactttacat ataagtgatg aaaactatcc tgctgttata aatgaaaaag aattagtaga
     1081 tacagttata tcaagtacta aagaattact tggagaggaa aaatttatat tgagaccaaa
     1141 cccgtcttta ggtggtgaag acttttcttt ttatacagaa cattgtaaag gagcattctt
     1201 ccatttagga tgtaaaaatg agaaaaaggg cttaatttca cctcttcata cctctagctt
     1261 taatatagat gaagattgtt taccaatagg tgttatgatg catgtaatga atactttgta
     1321 ttttaattag tacagtaatt aataaaagaa taggagataa attaatatgg aaaaagctaa
     1381 gaaattaggt ctatttagta tgatattatt agggattaat agcataatag gttcaggtat
     1441 attcttacta ccaggtaaag tttataatct agcaggtcaa aacagtatgt ttatatacat
     1501 ttttgctaca ctcttagttc tatcaatact tttatgtttt gcagaagttg gaagcatgtt
     1561 tgataagaat ggaggagcat atctatactc caaaaaagct tttggagatt ttataggatt
     1621 tgaagttggt acaatgtctt gggttattag aattatatca tggtctactc tagcagtagg
     1681 atttgcaaca gctttaggtt cattttggcc agaatctgcc actgaataca aaggttatat
     1741 agctgcaata ctcgtaacat tactttcaat aaatagctta tttggaatta aaagcacaaa
     1801 gataatgaat aatgtaataa ctatagctaa attagtacca ttaatagttt ttataatagt
     1861 tggaatattc tttataaaat ttgtaaatat tgttccttca ggaaatgtag ttaactctag
     1921 tatgggtcca gccattatat tagtatttta cgcatttacc ggctttgaat catttattgt
     1981 ggcaagtgga gaaatggaaa atcctaaaaa aaatcttcca gtcgcattaa taaccacaat
     2041 atttatatgt gcaataattt atatattaat tcaaattgtc tgtatgggta tacttggaga
     2101 tagactattt gaaaacagta ttccaattgc agatgcttca agtgtatttt taggcaatta
     2161 tggtaaggtt ttcatatctg tagcaacatt aatatctata tttggtataa atataggttc
     2221 atcaatagta actcctaaat gtggttcttc tctagcagaa gaaggttctc ttcctgcatt
     2281 tataggaaaa acaaataaat atggtgctcc atatgtagca ataataatat ctttaatttg
     2341 ttgtatacca cttgtgctaa ctggaagttt tgaacaactt gcagtaatga gtgtaattgc
     2401 aagatttgct cagtacatac caacttgttt atcagtaata gttttaagaa aaagaacaga
     2461 tgttaaggcc tcttttaaga ttccatttgg ccctgttata ccaatagtag ctattttagg
     2521 tagcttatgg ttgttacaac aagcttgggc agaagatatt tcaaaaccta taactcaaaa
     2581 tagagtttta ataggattag gggcaatgtt gttaatagct cctctatata tatttatgaa
     2641 aaaaaataaa gaaatcgagc ataaaaataa atcagaagat tctaaattat atatttcgag
     2701 agaaaaaatt caataatatt ttatactatt acttctcat
//
LOCUS       DACXMZ010000329         2713 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.330, whole genome shotgun sequence.
ACCESSION   DACXMZ010000329 DACXMZ010000000
VERSION     DACXMZ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.330"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            188..2323
                     /gene="rnr"
                     /locus_tag="JD016_18640"
     CDS             188..2323
                     /gene="rnr"
                     /locus_tag="JD016_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAU5001638.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1175 a          256 c          509 g          773 t
ORIGIN      
        1 acatcataat ttgtttgaag ttagtatgac aaagcttaca ttaaatcaaa aatataaata
       61 agtaatttta acttataata ataagaaaat atagaaaatt atgtgataag taaaaaatgt
      121 tttgtctgaa gaaatataaa agacaatata aataataata taatgaaaag taaaacagga
      181 gggaaaaatg ataccaggac ttaaagaaag tttgctagga ttaataaatg aaagtgcata
      241 taatcctcta aaaaaagaag aattagcaga aatatttgat atacattcat cagaaatgcc
      301 aatgttttat aattttttaa atgaattaga agaagatggt tatatctgct ttactaaaaa
      361 aggaaaaata gtatctccca atcaaatggg atactttgtt ggaaaatttg tatcacacaa
      421 aaagggtttt ggatttgtag aatctgatgt tgaatataca caggatttat ttatctcaag
      481 tgacaatata aatggtgcta tgcataatga tagagttatg gcagaaatag tagtacctgc
      541 aactgaagaa aagagagcag aaggaagaat agtaaaagta ataaagagag aaataactag
      601 aatagtaggt actttccaat caagtaaaac ttttggattt gtaacaccag acaacaagaa
      661 atttactaaa gatatatata tacctaaaaa acattttagt ggagcagtag ataatgataa
      721 ggtagtatgt gaaattacag tatggccaca agaggataga aaaccagaag gcaaaattat
      781 agagatacta ggtcaaaaag gtgaaagagg cgtagaaata gattctataa taagagaaca
      841 tggtcttcca gaagaatttc caaagaaagt attgcaagaa gcagaagctg tagctgttga
      901 gattcctcaa gaagagataa aaagaagaag agatttaaga gatttaaaca tatttactat
      961 agatggagat gacgcaaaag acttagatga tgccatatca atagaagtat tacctaatgg
     1021 aaacttcaaa ctaggagttc acatagctga tgttacacat tatgtaagag agaaaaataa
     1081 acttgacaag gaagccttaa aaagagctac atcagtttat ttagtagata aagtaattcc
     1141 aatgttgcca aaaactcttt caaatggagt atgtagctta aatccatttg aagataagtt
     1201 aactctttct atatttatgg aaatagacca taaaggaaat gtggttaaac atgaaatatg
     1261 tgaatctgta ataaattcaa aagctagaat gacttataca gaagtatctg atatattaga
     1321 aaaggatgat gaaaaactta aaaagacttt tgaacatgtt gttgaagatt ttaaaaatgc
     1381 tgaaatttta gctaggattc ttatgtcaag aagggaaaaa agaggagcta tagacttcaa
     1441 tttcccagag gcaaagataa ttctaaatgg aaaaggcgaa gttgtagata taaagccata
     1501 tgaaagaaga atatctaata agataataga agaatttatg cttataagta atgagacaat
     1561 tgccgaacat ttttactgga tgggaatacc gtttgtgtat agaatacatg agacaccatc
     1621 tttggaaaaa atggaagaat tgagtaagtt tatatctaca tttggatata taataaaagg
     1681 tgataaagag gaagtacatc caaaagcatt acaaggtata atagagaaaa taaagggcaa
     1741 aaaagaagaa ggagctatca gtactataat gcttcgttct ttaaaacaag ctaagtactc
     1801 accagaatgt agtggacatt ttggattagc agctaaattc tactgtcact ttacttcacc
     1861 aataagaaga tatcctgacc ttcaaattca tagaataata aaagaatctt taaataataa
     1921 aataagtgga aaaagacaag aacagcttac tactatagtg gattatgcat ctgttcaatc
     1981 atccgagaaa gaaagaaagg cagagttagc tgaaagagat gtacatgatt tctataaggc
     2041 tctttatatg aaggataaag ttggtcaaga atttgagggt gttgtatcta gcgtaacttc
     2101 atttggaata tttgttgagt tagataatac tatagaaggg ttgataagac ttgcaaatat
     2161 gaatgatgat tactatattt atgatgagag ttcttatagt gttttaggag aaagaactaa
     2221 gaagtcatat aagataggag accttgttaa gataaaggtt gaaagtgtta atgtagattt
     2281 taaagaaatt gactttgaga tagttgaaaa aatagaggag tagataaaac agctaaaata
     2341 agagataaca agttgtagta tttatgatat gtataaatat atagtgcaca atactctata
     2401 aaataaatga taaaaatagg taattgagaa atatctaatg gaaatagtta actgaaagat
     2461 aaataattaa gaaatattta ttgaaagtag ctaattgaaa aataagtaat tgagaaatgt
     2521 ttaatagaag tggctaattg aaaaataagt aattaagaaa tgcttaatag aagtggctaa
     2581 ttgaaaaata agtaattgag aaatgcttaa tagaagtggc taattgaaaa gtaagtaatt
     2641 aagaaatgtt taatagaagt ggctaattga gaaataaata attaagaaat gtttaataga
     2701 agtgactaat tga
//
LOCUS       DACXMZ010000330         2665 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.331, whole genome shotgun sequence.
ACCESSION   DACXMZ010000330 DACXMZ010000000
VERSION     DACXMZ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.331"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            65..763
                     /locus_tag="JD016_18645"
     CDS             65..763
                     /locus_tag="JD016_18645"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAU5001639.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            1206..2180
                     /locus_tag="JD016_18650"
     CDS             1206..2180
                     /locus_tag="JD016_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU5001640.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKELSLKAKGLLSQMLSLPEDWD
                     YTLAGLSLINRESIDAIRTAVWELEKAGYITRRQGRDEKGKMTAIEYTIYEQPQPPAL
                     DCPVLENPTADKPILENPTPDNPTSENPTQLNKELLKTNLPKKEKLNTDVSSTHSIPF
                     HSLNPSPLEDAAQPPERKRKEATDAYSVYEEIIKDNIEYDYLIQDRYLDRDRIEEILA
                     LILETVCTKRKTIRIAGDDYPAELVKARFMKLNSEHIRFVLDCMQENTTKIRNIKQYM
                     KAALFNAPSTIGSYYTSLVSHDMADGTFYGKPKKSGIPDYTCNEGESL"
     gene            2205..2423
                     /locus_tag="JD016_18655"
     CDS             2205..2423
                     /locus_tag="JD016_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001641.1"
                     /translation="MTQKTGALIFDETADRYDIRFDVNDYYGGLHCGDCMEVFVRGKW
                     KPTRMEYGDNWYLVGIRAADLSGLRVRI"
     gene            2517..>2665
                     /locus_tag="JD016_18660"
     CDS             2517..>2665
                     /locus_tag="JD016_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAU5001642.1"
                     /translation="MQEETNEKTIALYIKTGKLTAQQLQKAMKALLAQMKKQHDRQKI
                     PHGKQT"
BASE COUNT          905 a          548 c          531 g          681 t
ORIGIN      
        1 tttatgcttt tatagatttt tattttatac ataaatatat ttatatataa aggagataat
       61 ttttatgaat gaaaaattaa ttggtatttt ggctggaatg ggtccaaggt caacagcccc
      121 ttttgttgat ttaattattg atgagtgtca atctcaatat ggagcaaaat atgatgatga
      181 atttccaaag atgatgatat attcattacc taccccattt tatattgacc gtccaataaa
      241 tcatgaactt atgaaagaaa ctataattga tggattacaa aagcttgaat caactggtgt
      301 aaattttatt gcaatgccat gcaattcagc acatatatac tttaaagaat taaaagagtc
      361 tattaatata ccaatattaa atattgtaga agaaacagtt aaaaaattac ctactatctc
      421 acaaaaagta actctttttt cgactagttc aacttttgag tctacaattt accagaatgg
      481 tataatacat aatggacatg aatttatatt taaagatgaa tggcaaataa aattaaacaa
      541 tcttattcaa aatataaaaa tggataaaga aaatccacac aatattgata tttggaataa
      601 acttatcgaa gacgttaaaa atgaatctat agaaaatata gtaattgctt gtactgattt
      661 aaatgtagtt ttagaaaaat catatccttc aataaatatt gttgattcat caaaatgctt
      721 ggctgaatca gttgtcaata aatatctaaa attagttaaa taaatttttc atgcactata
      781 ttataaaaaa tatatgttat aagttttgtt aaaattagtt gattgtcttg aaaaatgctt
      841 gatttacggg catattcgca ggtttgcaaa ttgattttac tactcattta ctacttttaa
      901 cggattgagc cttgatatgc aaaaagcacc tgcgggctgc cggatagacc gctacaccac
      961 cttacgcggc acgtcgaaaa gaaaaaataa aataagcacc acctataagg cggcgtttct
     1021 cactcctaca ctggagaaca ggaacgctgc ttttttatgc cctcatgtta cgcagtaagg
     1081 gcaaatgaag ccttgattta tgcggctctt agagcgcgga aatgagaaat gcaagggttg
     1141 ataccttttc cctcaaaacc gcgtttctac tgcgtaacaa atccaaccaa aggagtgatg
     1201 aagctatggc agttttccgc gtggaaaaga acaaaggtta tacggttatg agcaaccacc
     1261 atttacgcaa caaggaactt tccctaaagg caaagggctt gttgtcgcaa atgctctcac
     1321 ttcccgaaga ttgggactac acccttgcag ggctgtccct tatcaaccgg gaaagtatcg
     1381 acgctatccg caccgctgta tgggagcttg aaaaagccgg atatatcaca aggcggcagg
     1441 gacgcgacga gaaaggcaaa atgaccgcta tcgagtacac catttacgaa cagccacagc
     1501 ccccagcatt ggattgtccg gtattggaaa atccaacagc ggataagccg atattggaaa
     1561 atccgacacc ggataacccg acgtcggaaa atccaacgca attaaataaa gaactattaa
     1621 aaactaactt acccaaaaaa gaaaaattaa atacagatgt atcaagtacc cattccattc
     1681 ctttccattc cctaaatccc tctcccttag aggacgcggc acagccgccg gaacggaagc
     1741 gaaaggaagc gacagacgca tacagcgtgt atgaggaaat catcaaggac aatattgagt
     1801 acgactatct gatacaggac agataccttg accgggacag gatagaggaa atccttgccc
     1861 ttattcttga aaccgtctgc accaaacgaa aaacaatccg tatcgccggg gacgactacc
     1921 cggcagagct tgtaaaagca aggtttatga aactgaacag cgaacatatc cgctttgtac
     1981 ttgactgtat gcaggaaaac accaccaaaa tccgcaacat caagcagtac atgaaagctg
     2041 cccttttcaa tgccccgtct acgattggca gctattacac gtcccttgta tctcacgata
     2101 tggcagatgg tactttttat gggaaaccga aaaaatccgg catacccgat tacacctgca
     2161 acgagggcga aagcctgtaa accaacccaa aggaggattt gattatgaca cagaaaacag
     2221 gagctttgat ttttgatgaa accgctgacc gctacgacat tcgctttgac gtaaacgact
     2281 actacggggg cttacattgc ggcgactgca tggaggtctt tgtgcggggc aaatggaagc
     2341 ctacccgtat ggagtacggg gacaactggt atcttgtggg tattcgggcg gcagaccttt
     2401 ccgggctgcg ggtacggatt taacagggcg gcgtgaaaac ggacgctctt ttttcatgcc
     2461 cgccccgctt cccggcggcg ggcataccac catagaacat gaaaggagga cacccattgc
     2521 aggaggaaac caacgaaaag accatagccc tttacatcaa aaccgggaaa ctgacggcac
     2581 agcagctcca aaaggctatg aaagccctgc ttgcacagat gaaaaagcag catgacagac
     2641 agaaaatccc gcatggcaaa cagac
//
LOCUS       DACXMZ010000331         2659 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.332, whole genome shotgun sequence.
ACCESSION   DACXMZ010000331 DACXMZ010000000
VERSION     DACXMZ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.332"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(27..1004)
                     /locus_tag="JD016_18665"
     CDS             complement(27..1004)
                     /locus_tag="JD016_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HAU5001643.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(1210..1473)
                     /locus_tag="JD016_18670"
     CDS             complement(1210..1473)
                     /locus_tag="JD016_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001644.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQTMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(1543..2616)
                     /locus_tag="JD016_18675"
     CDS             complement(1543..2616)
                     /locus_tag="JD016_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HAU5001645.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
BASE COUNT          794 a          522 c          265 g         1078 t
ORIGIN      
        1 atattaatta atttaagcag tatttattaa agttcaaatc cttgctctat aatcattttt
       61 ttatcatttt caatcccact tccaacagtt gtcaaataat caccaacaat tgcagaattt
      121 actcccccat taaatccaac tttgtcatat ccctttaatg atagccttcc accagcatac
      181 cttatgtaca cattaggaat tataaaccta aatactgctg ttgtttttag tatttccatt
      241 ggttctaata catcataatt ttccattggt gtattcttaa taggatttaa aatgtttatt
      301 ggaaatgatt ttacatttaa ttctcgtatt tcaaaagcca tttttagtct ctgttcttca
      361 ctctcattca ttccaattat accgcctaca catacatcta gcccagcttt tttagcatct
      421 tttatagttt ttatcctatc tttatatgtg tgtgttgtac atatattatg aaaattatct
      481 ctacacgtct caacattgtg gtgatatgta gtcacacctg ctgacttcaa cattttagct
      541 tgctcataat ctataatacc taaagatgca cataatttta aattagtatt ttccctcaat
      601 ccttcataga tatttaaaat attattaaac tcctttcctg acataccttt accacttgtg
      661 accaatgaga atctatgcac tcctttactc tccatctctt tagctctatt taagatttca
      721 ttgtaatcta ataatgaata ttcttctaca ccagttttat aataagaaga ctgagcacaa
      781 aatttacagt cttcactaca tttgccagat ttagcattca ttatcgtaca taaatctgct
      841 tttcttccca taaaatattc tcttatttca tttgcagatt ttaatagaat atcaaaatca
      901 tttttattat ttgtatcaag gcttattaaa ttaagagctt cttcataact tatttctttt
      961 tctctcaata ctttattttt taattttatt atgtattttt ccatatatcc tcctatttgt
     1021 caacttaata tttttatgta gtttacttat tatatatagt attgcatata taacatttat
     1081 tggtcaactt tattttagac acaagtttac tatacacaaa aaaatactgc ttcaaaataa
     1141 tattagtaaa tggattatac atattattat taaagcagta ttttttatta gaatataatt
     1201 cttactggtt tataaaacta tccttgtctt aactccatcc aagctttcca ttgtaataac
     1261 ctctaagcct tcatattctc taaatagcca ttttaaatcc ttcatggttt gattaacctg
     1321 actttttgtt agattattta taaaatattc ttttctagtt tcctcatact ctttgctagt
     1381 caaaaagttt cttagaaaat taaacgcaaa tacacttgga atattgttta cattagcctt
     1441 cttgctgttt tccttctcat agtaaatttt cataaatgca tactcctttg atacttttag
     1501 atttctctta caataattat atgcatttaa gtgctactat tcttataatt ttattaattc
     1561 atattcttta cttcccatac ccatttcaca tgcatagtct attattctag ttccatttac
     1621 atgaggatgt tcatgtttga aaacatcatg acccatttcc ttacaaatca aatcatagca
     1681 tgcttgttca atagctacag ggtctgttga tgctaatatt cctatatcat gggcaattgg
     1741 tctacctgac caaggtacac aatcacaaag tggagttaca ttcataacaa aagttatata
     1801 ccctaccttg tcttttttat ttgatactgc tccataagcg tattctgcca tcttttcgac
     1861 aaacacatct gagtcacttt cccattgtat agttactgct ctagttggac agacagttgg
     1921 acattctcca catccataac atacatcaga atctataact gcttttttat ccactatact
     1981 tatagctttt gtaggacaag aattaacaca ctttccacaa cctacacact tcttttcctt
     2041 tactactgga gtaacatcag aatgttgcat ttgttttcct gctgcacttg cacatcccat
     2101 agctaagttt tttaatgctc ctccaaaacc tgctgcttca tgacctttaa aatgactcat
     2161 tactatcatt gcagaagaat tataaatttc tccacctatt tttacacttt caaaatgttt
     2221 tttgtctatt tttacattct cataatttct actatataat ccatctgcaa ttattactgg
     2281 tgcatttaca actgcataag caaatccatt ttctatagca gttgtaagat ggtctacaga
     2341 atttgttcta ctccctgtat acagtgtatt tgtatctgtt aaaaatggct ttccttcgca
     2401 gtctttaacc ttatcaacca cttgtctaac tgccacagga ctcatatatg tagtatttcc
     2461 cttttctcca aagtgaagtt taattgcaac ttggtcattc ttttctatta aatctttgaa
     2521 tcctgcttca tcaaataatc ttcttacatt gtttggtacg tttttatttt gcgaatttga
     2581 gtataaatca caaaaatata cttttgactt ttccatatct acaccccttt aaaatattta
     2641 cttatactaa ttttatcac
//
LOCUS       DACXMZ010000332         2627 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.333, whole genome shotgun sequence.
ACCESSION   DACXMZ010000332 DACXMZ010000000
VERSION     DACXMZ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.333"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>2627
                     /gene="cbpA"
                     /locus_tag="JD016_18680"
     CDS             <1..>2627
                     /gene="cbpA"
                     /locus_tag="JD016_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HAU5001646.1"
                     /translation="GNAEEDSNAGNAEGDANAGNAEEDMNALVVEEATAEDPTSVGDF
                     KISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNI
                     KTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLIIDDSVVN
                     RDIEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGG
                     NTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGTITAKGAYHG
                     AGIGGGYYTTTKNALSPSVVDSSTGSGYSGNITINGGLSYSYGGAHGSAFGDGCVNPG
                     TNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLGGAVAYGD
                     LEKKTKVFMNKISLKYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDDQGVLYLWFS
                     DQFKGSEVSVNLVVIDKITGGNIKPDDFYVKDIGSGSQFLKQYEEFTIDDTPPYLIIK
                     RYDGLPFEEDVEKSFLDWILKDGGIPIITPKGEKLTDKKFMTISSQRLNEETMEPDTN
                     LGSDDLTKQVDAGKYQLSITSTQFSNVYPFNEAYWGHRAYYKAEIIPADTETSLVVEE
                     SKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVELKTHNKG
                     SESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLKLDVTNVD
                     VDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNGDGIWKP
                     DKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPNLNKPDPD
                     NGGNSKPDPDNGGNSKPDP"
BASE COUNT          987 a          310 c          578 g          752 t
ORIGIN      
        1 aggaaatgca gaagaagatt ctaatgcagg aaatgcagaa ggagatgcta atgcaggaaa
       61 tgcagaagaa gatatgaatg cattagttgt ggaagaagcc actgcagaag atcctacatc
      121 tgtaggagac tttaaaatta gtggtggaga aataaatgtc gattttaaat ttgcaagtgg
      181 cgttttaact attcttagtg gaaaacaaat gacaattcaa aataataatc caaatactcc
      241 aacaacacat aggattgtta tacctaaaga tgtaaaagct gaattaatat ttgatggagt
      301 aaatattaaa actgcatcta atagcccatt tactttaaca ccagataaag atggtggtgc
      361 atatgcacat gttatattac aagatggtag tgaaaacaag ctagagtcag gtgatgtata
      421 ttatccaggt atgagagcag gaaaaggtac tactctaatt attgatgatt ctgttgtaaa
      481 tagagatatt gaaggagagt ttatagttcc tgagggtgga aaaattccta gagatgtaac
      541 tctagaaaat ggagacaagt taaaaaaagg agatcgcctt acactactag atagtaataa
      601 tagaggaagt ttaaaagttg ttggaaatta ttattctgca ggtattggcg gtggaaatac
      661 tgaaagtgga ggaaatatta ttattaatgg tggtgctata actgctatag gtcaagaatc
      721 gagtcgtaat acaggatatg gagctggaat aggtggaggc ggtaatggta gtggtggaga
      781 tatcacaatc aatggtggta ctataactgc taaaggtgca taccatggag ctggaatagg
      841 tggaggatac tatacaacaa caaagaatgc attaagtccc tcagtagtag attctagcac
      901 aggttctggt tatagtggaa atattacaat taatggtgga ctttcctatt catatggagg
      961 tgcacatggt agtgcatttg gggatggatg tgttaatcca ggaacaaata gaggatatcg
     1021 tatacttgta actggaggaa cgattttacc ttatatgaca ggagtgggag attggatttt
     1081 tgacctaagt gctacaacgg gagatgttat agtaacaggt ggttcattaa aagcaacaac
     1141 attcaaaagt ttaggtggag cagttgcata cggtgattta gaaaagaaaa caaaagtttt
     1201 tatgaataaa atatctttaa agtatttagg acaagataaa gttagtacta cattagttga
     1261 tgattttaaa atgctaattg atggaaaaat ttatccttat ggagcaccta gttatacaga
     1321 tgatcaaggg gttctttact tatggttttc tgatcaattt aagggatctg aagtatctgt
     1381 taatttagtt gttatagata aaattactgg aggaaatata aaaccagatg atttttatgt
     1441 taaagatata ggttcaggtt ctcaattttt aaagcaatat gaagaattta ctattgatga
     1501 tactccacca tatttgatta ttaaaagata tgatggatta ccatttgaag aagatgtaga
     1561 aaaatcattt ttagattgga tattaaaaga tgggggtatt ccaataatta ctcctaaagg
     1621 tgaaaaactc acagataaaa aatttatgac tattagttct caacgtttaa atgaggaaac
     1681 aatggaacca gatacaaatt taggaagtga tgaccttaca aagcaagttg atgctggaaa
     1741 atatcaatta agcattacat caacacagtt ttcaaatgta tatcctttta atgaagctta
     1801 ttgggggcat agagcatatt ataaggcaga aattattcct gctgatacag aaacttcatt
     1861 agttgtagaa gagtctaaag ctaaaactga tgtttttaaa ccaacagata cgtttacatt
     1921 aaatgttact atttcaccag ataagaaaga aggaccagat tgtgcctctc caagaggtta
     1981 cgtacaattc tatattaatg gaaaggaata taaagatcca gtagaattaa aaactcataa
     2041 taagggtagt gagagtcata attatagtac tgcatctatt gaatggagac ctttagatag
     2101 caatcatcat atttttacta ctgaacaaac tattactgta aaatatattg gagatgatat
     2161 aaattacatt gaaggtagtg aggatgaaaa gaagttaaag ttagatgtga caaatgtaga
     2221 tgtagatggg gatggaatac cggatataaa tatagacact gatggtgatg gaaggccaga
     2281 tatcaatata gatacgagtg gtgattggaa gccagatatt aacatagata ctgataatac
     2341 aggtgaatgg aagcctagta cagaaggtgg aaatggagat ggtatttgga aacctgataa
     2401 aaatatagat accaatggag atggaaaccc tgatacagat tataatcgtc cagcaataga
     2461 tacagataat gatggagtag atgattattg gaagcctgat aaaaatgtag atactggctc
     2521 aggaggttat gatacaggta atccaaatct aaataagcca gatccagata atggaggaaa
     2581 tagcaagccg gatccagata atggaggaaa tagcaagccg gatccag
//
LOCUS       DACXMZ010000333         2627 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.334, whole genome shotgun sequence.
ACCESSION   DACXMZ010000333 DACXMZ010000000
VERSION     DACXMZ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.334"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            39..1238
                     /locus_tag="JD016_18685"
     CDS             39..1238
                     /locus_tag="JD016_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001647.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILSSKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            1244..2059
                     /locus_tag="JD016_18690"
     CDS             1244..2059
                     /locus_tag="JD016_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001648.1"
                     /translation="MMKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNY
                     IIENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTELWFMFGIIACICYAFFIWYRD
                     FNGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGN
                     VTNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLIS
                     FLFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            2070..>2627
                     /locus_tag="JD016_18695"
     CDS             2070..>2627
                     /locus_tag="JD016_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001649.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNIS"
BASE COUNT         1127 a          206 c          392 g          902 t
ORIGIN      
        1 ctaattaaaa agaaagtatc tctttaagga ggggtggtat gaaattaaaa tctaaaatat
       61 ctatacttat tatatttttg actttagcaa tatattttag tataggttta ttaaataatg
      121 aagataattt aaagataaaa actataaaag gtgactctaa tgaattagac catataaaca
      181 taaattttgg atatgctttg aatggatata taaattcata ttatacaata aatggaagca
      241 acaaaactaa aaagactgaa ttaataaaac tacaaaagga tagtaagaac acaaataatt
      301 taattgatgt atctagtggt tcagatttaa ttgctcatgt tggatttgaa atgaatgagg
      361 agtcaaaaga tataaacttt gtgatagatg agaaaaaaga agatttagaa aaaggtgtat
      421 ttccatcaga aggtagtgat aaagaaattg aatatgaaaa attagatatg aataatttta
      481 caaggacaag atataaagta gataaaagta cagatgatta ttatgcaaag atactatcat
      541 caaaaagata taaaaaatac ttatatgtat ctgtacttta tgctacaagt gattcatcag
      601 aaaataacaa agaatattca agtactatag atataataaa aattaatcca gaaaataaaa
      661 aaatcaagac tataaaaagt ataaatttaa gtaattattt ttccgaaaaa taccaaaaca
      721 tagagaacat aggtgtaagt tatgctagca aatatgagga taagatgtat attttagtaa
      781 atgtactaaa tacaaacata ggtgataata gtaattctaa atttcattta cttgagtata
      841 atcttgaaga aaataaggaa agcataatgg aattaaaaac tgatagcaaa atggctttag
      901 caaaagcaaa gttagaagct aataaattat atatggtaat gtcgaatgat tatgataata
      961 gttcaattac agagttaaat ttacttacct ataatatatt agataaaagc ttagatgaag
     1021 ataaatatgt tttagataat agagaaaaaa gaggaattag aagtattgaa attgattcaa
     1081 ataaaatcgt tgcatataca tcaaggctaa actctatatt atcttgggat gataaattta
     1141 tgctatatat aataaataaa aattctaagg aaattgtata tgaaggagaa ttacaattta
     1201 acagttcaaa tagtatgtta gaatttgaaa taaagtaggt gagttgatga aagatttaat
     1261 aagattattc gaatttgaat taaaaagaaa tataaagaat tatatattta taataatttg
     1321 ttgttgttct tttgtaattt taaacatagt aaaaaattta aatgactata attacataat
     1381 agaaaatgca gttaaaagtg aacagttaac aaaaatcgga aatacgatag aaacggtaaa
     1441 tgttacaggt ttctcaagct ttagaaatat aatgggttct acagaattgt ggtttatgtt
     1501 tggtataatt gcatgtattt gctatgcttt ttttatttgg tatagagact ttaatggtag
     1561 aagtaaatct atatatacac ttataatgct acctaaaaat agaatcaata tttatatatc
     1621 taaattatta aatatattat ttctagtgta tagctataca gtagtactta caatatcttt
     1681 gtttattgca agtaagttgt tacctagaca tatgcttggt aatgtcacaa actatggttt
     1741 tgtacaagaa acaatatatg agttaaaaat gttacttcca tatagctttg aaatactatt
     1801 tgtagagtat atatttttat tgataggatt tgtatctgta gtatttacat caattcttat
     1861 aaataaatct atttataaag taagcacttt aataagtttt ctatttttag ttatagaaat
     1921 tttagtgttt ataatttcta tagaatttat tataccttac gaatacagtg atgtatttac
     1981 tgttctatat tcaagtataa acatatttgt atgtagtctt atatctaata aacttttgaa
     2041 gcaaaagatt gacttttagg agggatatta tgaaagcaaa aaatatagga aaatatacaa
     2101 tatgttttat aatagtatgt tttttaatga ttactatgac cttaatattg agttatttta
     2161 ataaggataa actatctgta aaaactttaa aaggcaatgt aaaagatata gggagagtaa
     2221 gtgtagtata tacacctaat ataagcaaat tttcttatga ggaggtaatt ataagtaaaa
     2281 atggagtaag aaataataca aacccaaaat caagaattag aaatgattat ttaaatattg
     2341 acaaaaaata cagtgacttt gttaatgata aaggaaaaga atttatatat aaagatgatt
     2401 tgaatatagg agaagcgtat acctctgtta agtctgattt agtacaagga atgaatgctt
     2461 taaatgattg tataaaggtt tcaaataaaa atttaaaaag tggggtagaa gaaaattttg
     2521 atattaattt aaataagtct gattttaaag aaaatagttg gaaaacctta tttaacaaaa
     2581 tatataaagg taatttatat atggtaatta gtgaaactaa tatttca
//
LOCUS       DACXMZ010000334         2627 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.335, whole genome shotgun sequence.
ACCESSION   DACXMZ010000334 DACXMZ010000000
VERSION     DACXMZ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.335"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            119..2137
                     /locus_tag="JD016_18700"
     CDS             119..2137
                     /locus_tag="JD016_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAU5001650.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIR
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT         1092 a          303 c          435 g          797 t
ORIGIN      
        1 tagaagttta ttttttatgt acttatttat aattttgggt aaaaacactt tgatataatt
       61 tcaaaaatta aaaggttaaa aaacaaagta gaaatttatg ttaaataagg agattaatat
      121 gagaagaaat acaaaattat taacaacagg gattctttca atggcaatcg tcgcacctac
      181 aatggcattt gctactgaat ctaatgctat ggaaaataac gctgatttaa atataaactt
      241 agagaaaaaa agtatcgttt taggtagtac ctcaaaagtc agtgttaagt ttaaagaaaa
      301 accagatgca gatagcatta cattaaagta taaatgctat gatatgccat tggatacaac
      361 tttaaactac aatcaatcaa ctgagtcata tgaaggaact atcaattata ataaagaccc
      421 agaatatcta aatgtttggg aactacaagg gataacaata aacagcaaaa ataatcctaa
      481 aactttaaac aaacaagaac tagaaaagat gggattaaat ttaaaagact ataatgtaac
      541 acaggaatgt ataattgaag atataacttc tagaaaagat gtaaataaat atttaagaaa
      601 aacttctgca cctattacag aacttacagg aagtgataga tacgaaacag cagttaaaat
      661 aagtaaagaa ggctggaaaa atggttcaga taaggtagtt ataataaatg gggatgtaag
      721 tatagatggc attatatcaa ctccactagc aactacatat aatgctccaa tacttttggt
      781 tgaaaaaaac aatgtaccta atagtgtaaa atcagaatta aagcgtttaa atcctagaga
      841 tgtaattata attggagatg agaatgctat ttctaaaact actgctaatc aaattaaatc
      901 gactgtaaat gctagtcaaa cacgtctaaa gggctctaat agatatgaga catctttatt
      961 gatagcaaag gaaatagata aaaatcatga tgtggaaaaa gtatacataa caaatgctaa
     1021 tggcggagaa gtggatgcac ttactatagc agcaaaagca ggtcaagaca agcaaccaat
     1081 tatattaact gataaaaata gtattacaga caatacatat aaatggttaa agagtgagga
     1141 tttacaaaat gcttatttta taggtggccc tcaaatgata tcaacaaatg ttataaataa
     1201 ggtaaatgat ataactaaag ataatgttac taataataga gtatatggag cagatagaca
     1261 cgaaacaaat gcaaacgtaa taaaaaaatt ctatacagat gatgagttag aggctgtttt
     1321 agtagctaaa tcagatgtac ttgttgatgc tttagcagca ggtccattgg ctgcgaactt
     1381 aaaatctcca atacttataa caccaaagac atatgtatct gcataccata aagaaaactt
     1441 agaagctaaa tcggcaaata aggtatataa aataggtgga ggattgactt ctaaggtaat
     1501 gagctctata gcatcatcat tatctaaaca caatacgact ccaacagaac caggaaatag
     1561 tgggggcaag acagttatga ttgacccagg acatggtggt tcggacactg gaacaacagg
     1621 taaaccatta gggggtatta gagaaaaaga ctatacatta aatacttcac ttgcgacaac
     1681 tgaatattta cgttcaaagg gattcaatgt aataatgaca agagacacag ataagacttt
     1741 atctcttgga aatagaactg ctttatctaa ctctttgaga ccagatttat ttacgagtat
     1801 acactataat gcatctgata caactgggaa tggtgtagaa gtattttata agcttaaaga
     1861 taaagatgga ggaactacta agactgtagc tacaaatata ttaaatagaa ttttagagaa
     1921 atttaatcta aaaaatagag gtgcaaaaac aagaacacta tcaactgacc ctacaaaaga
     1981 ttatttatat gttttaagaa ataatgatat gccagctgta cttgtagaat gtgcattttt
     2041 ggataatgag aaagatatga gtttacttaa tacatcaaat aaagtaaaag aaatgggtac
     2101 acaaataggt aaaggcatag aagattcatt aaaatagtct acaatgagag catagaaaaa
     2161 tttaaaataa attaatctgc aattttaaat ttaaactaaa gctataagat aagaatgtaa
     2221 ttataatagt aataaagact gtatatacat tgtgcatata agtgcgaaat gaggtatata
     2281 cagtcttttt atgattaatt gtaaaaggat ttatattata tgaagaattt acatatctta
     2341 aggctacagt taattgttaa gagttggagt attcatttgt aatacacaat ataagcttaa
     2401 ttactatatt ttattgtgtg tttgtgtgtt ttttgtggat ttttatgtca ataaatgata
     2461 tagtatagat aattaaatgt attaaatata tttaattttt ttattttttg agttttataa
     2521 gtttattttt atatattact tatatattat ttatattttg ggttagatac actttgacat
     2581 attttcaaaa gttaaaagat taaaaataaa gtagaaattt atgttaa
//
LOCUS       DACXMZ010000335         2613 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.336, whole genome shotgun sequence.
ACCESSION   DACXMZ010000335 DACXMZ010000000
VERSION     DACXMZ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.336"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(216..1466)
                     /locus_tag="JD016_18705"
     CDS             complement(216..1466)
                     /locus_tag="JD016_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HAU5001651.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1498..2439)
                     /locus_tag="JD016_18710"
     CDS             complement(1498..2439)
                     /locus_tag="JD016_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAU5001652.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          788 a          496 c          244 g         1085 t
ORIGIN      
        1 taaaacctta taaatataca aatacaatta tatgaatttt catcctcaat tatagcatag
       61 aatatgacta tctatatcct aaaattatcc atttctaaaa aaaaggtaac tattattaag
      121 aatatcaact tatagaattt atattccaca tacgattttc aatcaaaaaa acaagggcta
      181 aaaagtgaat tgtgcacttt ttagcccttt attaattatt catttatttc atgtaagaat
      241 agaccacatc ttactttagg ttcaaaccat gttgacttag ctggcattat cttatttgca
      301 tctgctactc ttattaaatc ttcaatacta gtaggataca ttgcaaatgc tactttcata
      361 tcttcattaa ctcttttttc taattcttca agacctctaa ttccaccgat aaaatcaatt
      421 cttttatcta ctctaggatt atcaattcct aaaatagcat caataacata gtcttgtaat
      481 atagaaatgt ctaatgagtc aacagggtct tctatttcta ataaattttt atttgctttc
      541 attttatacc acttatcacc taggtacatt ccgaaagttc cttttttgtc aggtttatat
      601 ttttcttttc cttctatttc ttctagttca aaattttctt ttattttatt tataaattct
      661 tcctcgctta aaccatttaa gtctttgata accctattat aatccaaaac cattaaattc
      721 tcatctggtg caatcattgc tatgaagtaa ttaaattctt cttttccagt ataatttgga
      781 tttttagctc tcatttcttt ggctacattt tctgctgaag ctgtcctatg atgaccatct
      841 gcaatgtata gataatctaa tttttcaaat ttatctacta ggttatctat tatatcatca
      901 ttatcaatct tccatactgt atgctctatc ttgtcatctg ttatgaaatc atataaaggt
      961 tcattattat ccataaaatc attgattata tcatctatca ccctttgatg tttgtaagtt
     1021 acaagtatag ttcccgtatt agcatcacag tattttatgt gtttagttct atcaatttcc
     1081 ttttctggtc gagtgtactc atgttttttt attgttccat tcaagctttc atcaactgat
     1141 atacatgcta ctataccaac ttgagctcta ccatccatga tttgtttata tatatatata
     1201 cactctttgt catcttttac tagaatttct tgttttcgaa actcatctaa attttctcgc
     1261 gcttttaaat aaactttttc atcatataaa tctatatttt catctaaatc aatttctgat
     1321 ttatctatat gcaaaaatga atatggattc ccttctgcaa ttcttctagc ttctttacta
     1381 ttcataacat cgtaaggaag tgctgctact ttatctacta tatatttatt tggtctaatc
     1441 gccttaaatg gtttaatcgt tgccattact aatttcctcc taattataca aaatatttta
     1501 taatgctacc cctattagat tgttgaagtc aagaaagaaa tcttgaatca catttactat
     1561 ttcgtctcct attctttctt gagcttctac tgtagatgcc ccaatatgag gtgtaggaga
     1621 aactcttgga tgatttacta agtctaaatt taatgtaggt tcttgttcat aaacatctat
     1681 agcagctgct gataatttac cttcatctaa tgcttttagt aaatcttttt catcaactaa
     1741 accgcctctt gcacagttta ttaaataagc ccctttcttc atcttattta tttctttttc
     1801 agttattact gcccccttat tcttatcaaa tggtatgtgt aaagataaga aatctgcatt
     1861 ttctaaaact tcttccatat cacagaattt ataattgtta aagccttgcg cctcacccat
     1921 tatgtcagta tatataacat tcatacctaa taattctgct ctctttgcaa cttcttttgc
     1981 aattcttcca aaacctataa gtccaagtgt ctttccatta atttctgtac ctttatactt
     2041 tttcttctcc cattttccat ctctcatagt tacatttgca gtatttatga atcttgcaag
     2101 aacaaataat tgacctattg taagctctgc aacagatata ctgcttgcat ttggagtatt
     2161 cataactttt atgccattag cttgagcata tttaacatct atattatcca gacctactcc
     2221 acctcttact atcaacttca actttttacc ctctaatgct ttatctatta catctttagt
     2281 aactttagta gctgaacgta ctacaagtac atcaacatct ttaagtttat ctcctaaaac
     2341 atctttttca taaaactgct ctatcacatt aaaatctaac tctctcaatt ttctcgctgc
     2401 ttctttctct attccatcag taactaatat attatacata acaaactcct ccaattttta
     2461 ttcacttaat ttttccaatt tttatttttt aaagtatttt catcaataaa aaactttact
     2521 taattcaact ttctgtttat ataattttca aaatattttt ttattaaagt catatcaaat
     2581 ttttctattg tttgttaaaa atttaatatg taa
//
LOCUS       DACXMZ010000336         2603 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.337, whole genome shotgun sequence.
ACCESSION   DACXMZ010000336 DACXMZ010000000
VERSION     DACXMZ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.337"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            448..927
                     /locus_tag="JD016_18715"
     CDS             448..927
                     /locus_tag="JD016_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAU5001653.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            924..1703
                     /locus_tag="JD016_18720"
     CDS             924..1703
                     /locus_tag="JD016_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001654.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1953..2453)
                     /locus_tag="JD016_18725"
     CDS             complement(1953..2453)
                     /locus_tag="JD016_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU5001655.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT         1019 a          357 c          316 g          911 t
ORIGIN      
        1 aatgacattt ctaactctta attctcattt aagtttcatt gttttttctt aaaagcctac
       61 ttttagtcaa gatactgtta tctttactca ttatttaaag aacttatttt aaatctgtac
      121 atgaagtaaa taatccaaaa gcattgttat tctaactact caacattaaa tacttactat
      181 tttctaatta ctcaatataa agacttacta taaaactaaa tacataaatg gcttctaaag
      241 tttaatatat ctacatggct ttatatatga caggaaaaat actagaaaaa atatattata
      301 gaatacttgt atataatagg caaaactttc aaacttaaat ttatcaaata atcataaaaa
      361 aagtgaacca tctatagtaa aattttatct aataagtgaa aggcaaaaaa gtagcgctac
      421 gatttgcata gaagggaagg aacttagttg gataaaacta catttacaaa caatatactt
      481 gaatcagaac agactttgta tcgagtatct aagtctattc taggaaacga ccaagactgt
      541 gaagatgccg tcaacaatgc tattttaaaa gcatatgaaa agttggattc tcttaaggaa
      601 gaacaatact ttaaaacatg gctgattaga atagtcataa atgaatgtaa ttcattaaga
      661 cgcaaacgat taaaatctct atcatttgaa gatgtcttca aaaacaaaaa aattgatgaa
      721 aaagatgact atagcgattt gtatactgct attcaaagtc tacctaaaaa aattaaaatt
      781 ccaatagtcc tttactatat tgaaggttat tcagtagatg aagttaagga aatattagat
      841 atacctcaag gaactgtaaa aagtagatta tcaagaggaa gaagattatt aaaaactaaa
      901 ttagaaaaca cggaggtaat catatgaata aaaatatatt aaataattcg tttccagaag
      961 ttccagaaag ttttcataat agtttaagta aaacactaaa tagtttacca gaaagagagg
     1021 aaaattataa aatgattaat aataaaacac acaaattacc atttagaaaa ggcttagttg
     1081 caactttagc cattactttg atattgagca caacagccct tgcagtagga catatatata
     1141 gtttggttgg aaaatcaagt actaaggcta cctatacatc tatcccaact acagaacaag
     1201 taaaaaaaga ctttaacttt acccctaata tagtttctga atttagtaat ggatataaat
     1261 ttaaaggagc atatactgta aataaaaaag catttgacaa agaaaataat gttttaggaa
     1321 aatcaaaagc acttagtttt gaatataaaa atggcaatga tgaaatatct cttgacacag
     1381 caaataatgt acttgggaag agttctgaca atctaaaagt tgtagatact tacaaagata
     1441 ttaatatttc ttatgaagca ttcgcacaaa agtttgtacc agaaggttat aaaatgacag
     1501 aacaggataa aattgatgaa aaatcaggca aatacacatt tagtgtaggg ccaaataatg
     1561 aatcagacaa agttgaagtt aataaattta agtttttgac atggaaacaa gatggagttt
     1621 actattcttt tacagttcaa gactcaaatt tatctatgaa tgacttagtt aaaatggctc
     1681 atgaagtaat agatgcaaaa taaacataag taataataag ttgtttatga gtttattatt
     1741 tatgacttta atttttttat tagtaaacat ggttgtatct tccatcttta tattttcttt
     1801 aaatttaata tataaaattt tagatattta gctaccttca aaagaaccat tattaaaaag
     1861 cccttaaaaa tatttctttt atatggaatt ttaagggctt taactttatt ttatacaagt
     1921 gtatatggaa ttttaaagac tttaacttta ttttatataa gtgtaagctc catttgaatt
     1981 acttcttctt ttacaggtct atcattccat gtaaaaccac tagatattgt atttacctct
     2041 acaaacccta aagatttata aacatgaata gctctactat tacttttaaa tacatcaagc
     2101 tcaatttttt catacccata ttcttttgat aaatcaataa tttgttttaa aagactactt
     2161 ccgatacctt tttccctata ctctttaaga atacccatgc ctaaataacc aactttttca
     2221 gttttaggta aaacgtcaca ccaaccaata cagttatctg attctaaatc aattataaac
     2281 agttgtggta agtttttatt tataatattt tttataaatt caactgtttc ttcaaaagga
     2341 aaggcttcat ccatagctag atatttcctc tcttttgcta ttttatcaaa agtttgccaa
     2401 tacgaataaa tatacttttc ttctgccttt ataaatttta tgccttctat caacttaaca
     2461 tttccccttt ttaatttttg attgtagtta acttatttta tatgattcta ttttataatg
     2521 ctatataaat tactatatat tagcttaaaa tatctttgta tatttctata aaattaaaac
     2581 caatatatct atctcttgta tct
//
LOCUS       DACXMZ010000337         2590 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.338, whole genome shotgun sequence.
ACCESSION   DACXMZ010000337 DACXMZ010000000
VERSION     DACXMZ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.338"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..897)
                     /locus_tag="JD016_18730"
     CDS             complement(<1..897)
                     /locus_tag="JD016_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HAU5001656.1"
                     /translation="MCFRFMKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDN
                     EFNTIDNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDV
                     TIYNWGDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLL
                     NMKKIFSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYI
                     PYFEDENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLS
                     SSFKLIKLNEFEEYPDFILKLGD"
     gene            complement(922..1269)
                     /locus_tag="JD016_18735"
     CDS             complement(922..1269)
                     /locus_tag="JD016_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001657.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1487..2443)
                     /locus_tag="JD016_18740"
     CDS             complement(1487..2443)
                     /locus_tag="JD016_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAU5001658.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPIRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
BASE COUNT          882 a          376 c          294 g         1038 t
ORIGIN      
        1 atctcctaat tttaagataa aatcaggata ttcttcaaat tcattgagtt ttattaattt
       61 gaaagaagaa gatagatttt taaagttaat aattttagag ttattcacta atacacttct
      121 ttttttaaaa taaatttctt ttttacttat atttagtata gttaatattt gtttaagata
      181 tattattata tcttctggag ttctatcaaa tttgagttca aaattttcat cttcaaagta
      241 gggaatataa tataaaggtg ttttgagagc aggtaattta ttatataatt cttcaagaat
      301 atcgatattt ttaggggagt taaagaaaaa gcatttacat acattcatta aatccacagc
      361 atctgaaagt gagttatgtt taatatctcc ttctacagaa aaaatttttt tcatattcag
      421 taaagatatt tgcttagata agattctatt attgtatgta atatgttgag atataaaagg
      481 ttgaatgtca atcatcatat taaataactc taagcattta cctgtatatt tatatactct
      541 aaatgaagtt agaaggccag ttatatcaaa atcaccccaa ttatatattg taacatcatc
      601 aaatttacaa aaccatttat aaaaatcatc cataacatat aaaagattag gagcattatt
      661 gatatctagt tggttcaatt tggttaaatt tttacattta tttgaaagta ttttattgta
      721 ttttggctta actaaagagt aataattatc tattgtatta aattcgttat caactaaaac
      781 tccaccaata gaaattaatt ccgcaccatt atattctttt ggtttatttt tattatcatc
      841 tagtatatta aattcaaagt ctataaataa cttgtatttc ataaatctaa agcacatcct
      901 caggtaaatt atttattgag cctaagcatc ataagaatca atagatataa tgactctaaa
      961 catcaaaggt tcattacaac ttttatttat ttcataattt aattcaaata aatcttttaa
     1021 gatataattt ataagtattc tttcccaacc catttttaaa ttaactttag agtctattaa
     1081 tatattttct atctttttaa atatttctat atttgctggt attatactga gctcaatatt
     1141 acttttaaac gggttagact gtaaatctgt tataagtata ttttttaatt cctctgtaag
     1201 ttcagaaatt aaactatcac ctatgtattt ttttatgatt tcctctctaa tttcttttaa
     1261 atttagcaat attagtacct gctacttaca tagtagcttt tcctttcctt ttaatactat
     1321 tattcatttt tgaatcttat ttagatatat tttaagatta aaagtcttta gctatataat
     1381 tttttaccca tttttaactt atattaacat attacaggct tatatctcca ccctgcttac
     1441 attgaggatg gagtatattg cctattttga taaaaatgat aggttgttat ttgcctattc
     1501 ctttttctga taatttttct aacatatctt tggtcatttt atctaaatca taactaaact
     1561 taaagttcca ttcttttact gcactactac tgtctagaga gttaggccaa gaatcagcta
     1621 tactttgtct tataggatct acatcatatt ctatcacaaa atcaggaata tattttttta
     1681 tagaatcagc tatttcttca ggagaaaagc tcatagcagt aatattaaaa gcatttctat
     1741 ggatgagttt atcagctgga gcttcgatta aatccactat tgattgaagt gcatcaggca
     1801 tatacatcat atccattttt gtaccttctg ctatgtaaga tttatatctc ttatttttta
     1861 atgcctcata atatatatca actgcataat ctgtagttcc tccaccagga ggcgtaacat
     1921 aagaaataag acctggaaat ctaacacctc tagtgtctac tccaaatttt gagtgataat
     1981 aatcacatag taattctcca gaaactttag tcacaccata catagtatta ggtctttgta
     2041 atgtatcttg aggagtcata ttttttggag aattttcacc aaaagcagca attgaactag
     2101 gtgtaaaaaa tttacaatcc aattctttgg ctatttcaag accatttatt aatccattca
     2161 tatttaagtt ccaagcttct agaggtttac tttcagcaac tgcagaaagt aaagatgcta
     2221 gatgaattat agtatctaca tcatattttt ttgcaatttc aaaaaaacga ttcttatctg
     2281 taacatctaa tatctcaaat ataccagatt cacatactgg attaccttct tttactctcc
     2341 ttgaactagc gattacattt tgttctccat actcatttct aagctttatt gttaattctg
     2401 aacctatttg tcctaatgca cctgttataa gtattttttt caattttaca acctcctaag
     2461 ggtttaattt atgtagtact acttattttt attttagcaa atcttttgtt taaatacaaa
     2521 gaattaaaga taataatata cgacttaatt gaatgaagtg cagtaagtgc ctaaaaattt
     2581 aaagctaata
//
LOCUS       DACXMZ010000338         2531 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.339, whole genome shotgun sequence.
ACCESSION   DACXMZ010000338 DACXMZ010000000
VERSION     DACXMZ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.339"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            227..1846
                     /locus_tag="JD016_18745"
     CDS             227..1846
                     /locus_tag="JD016_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAU5001659.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2220..2414
                     /locus_tag="JD016_18750"
     CDS             2220..2414
                     /locus_tag="JD016_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAU5001660.1"
                     /translation="MINYMVEIIYSEKISEIENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT          948 a          290 c          373 g          920 t
ORIGIN      
        1 aaatactaaa aatctaagtt caagtattta aattgctata tatcataaat tttctaaaat
       61 taacaacaac ttaaaaattt aacattctct taacgtatac ttaacaaaac attaatattt
      121 ttattgacaa aatttgttat tctatattat ggaaacaata aattttagtt aataaagttt
      181 ataattcaga taaaaaaatt attaatttct taagggagga aatatcgtga atatagtaat
      241 tagccttatc ggtggacttg gactttttct ttacggaatg agtttaatgg gagaaggttt
      301 acaaaaatca gcaggagaca aattaaaaaa aattattgaa ctcttaacaa gtaatgttgt
      361 tatgggtgta ctggtaggta cagtcgttac aggtattata caaagttcaa gtgctactac
      421 tgttatggtt gtaggatttg taaatgcagg tataatgaat ctaagtcaag ctattggtgt
      481 aataatgggg gcaaatatag gtactacagt tactgctcaa ttagtttcat ttaatcttga
      541 aggaattgca ccaattgctt taggtatagg tattttattc tatttattta catcaaatca
      601 aaaaacaaag catttatctg agatacttat aggtttcggt atattattta caggaatgga
      661 gtttatgaaa gatgcagtag ctccacttgc tgaatataaa gcattcactg atgcactttt
      721 atattttagt aaaaatcctg tattaggtat attggcagga tttgccataa ctggtattgt
      781 tcaaagttca agtgcatcta tgggtatgtt aatagcacta gcttctcaag gaatacttcc
      841 tctatcatct gctttaccta ttctttatgg agataatata ggtacttgtg ttacttcact
      901 tttatcaagt gttggtgcaa gtagaaatgc tcgacgtgca gctgtaatgc atttaagttt
      961 taatgtaatt ggtacaataa tatttatgct tgttttaaat aaacctatat cagccatagt
     1021 tactcatttt gacccaactg atacagctag acaaatagca aatgcacata ctttatttaa
     1081 tcttacaaat gttataatat tattaccatt ttctaaatac atagttaaat tagcaaatag
     1141 acttatacct attaaagaaa ctgaatcaga aatagtaaat aatactaagt acttagatga
     1201 aaggatgttt agcactccat ctatagcact tggtaatact gtacaagaag ttgtaagaat
     1261 gggtcataaa gctacaaact ctttggaaca ttcaattgca ggatttttaa ataagagcaa
     1321 tgaagatatt aataaagctt ttgagtcaga aaaagttgtc aataagttac agaaagatat
     1381 attgaactat ttgttaaaac tttctaaaga acctcttaga gatgatgaac gctttagaac
     1441 agatttgctt tttaatactg taaatgacat tgaaagagtc tctgaccatg cagaaaatat
     1501 agctgaattg gccatgagtg ttaaggaaat gaacattagt ttttctgata gtgcgataag
     1561 agaaatatac gaaatataca ataagacaat tactaatttt aaggatgcct taatagtttt
     1621 agatgttaaa gattttgagt tagcaaataa agttttagaa gttgaaaatg aagtaaatta
     1681 tttagaaaaa acttttagaa attcacatat gattagacta aataatggaa gttgtacaat
     1741 tgatgctggt gtactttatc ttgacttact tactaatcta gaaagaattt ctgaccactc
     1801 aacaaatata gtaaaacaag tactaaaact taaacaaaaa atatagtgat tatttttata
     1861 tataatctta atcaatgaga agtaagaagc tatagataat gcttatatca tttatggctt
     1921 cttatttttt atgtaatact caaattttta tagtattctc ttattattaa acagtttctg
     1981 cctagaatat tccatatata tatgacttct ttcataaaac ttgaactgcc cataaataaa
     2041 gtcacaagta tacataacta ttttttaatc aattataacc aatatttgtt ttatttatct
     2101 tcattttaat tcttcattca ataatgtaat aagattattt tttatttgga tactattata
     2161 tcatctaaat atgttattat atatttggat attttcatat taataggagg gattctacaa
     2221 tgataaatta tatggtagaa attatatata gcgaaaaaat aagcgaaata gaaaacatgt
     2281 tgaaaattga aaatatacaa tatgaattta cttctcgtga tggctttata ttttttggtt
     2341 tttatgatat gacagacgca tatagatttg agactttatg ttcagatgaa aaaattcgat
     2401 ttaaaagaat ataatagata aaaaagtaca tatttctaaa ctatttgttt ttcaaattta
     2461 ggttgtgttt taatgtagtg ttgatattat aaacaatttt aataataaaa aagtagggac
     2521 attatattaa t
//
LOCUS       DACXMZ010000339         2528 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.340, whole genome shotgun sequence.
ACCESSION   DACXMZ010000339 DACXMZ010000000
VERSION     DACXMZ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.340"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..387
                     /locus_tag="JD016_18755"
     CDS             <1..387
                     /locus_tag="JD016_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAU5001661.1"
                     /translation="TGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEYLKI
                     SGNKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDELER
                     LKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            377..1774
                     /locus_tag="JD016_18760"
     CDS             377..1774
                     /locus_tag="JD016_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAU5001662.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAMNSIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIRHGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKYRPTLVNELHTKLTQSIIRGENPKNLV
                     NDFDKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKYVKHNSILEIKNSAIIDSIKEDIKNGKYNLNIHDGKQGKHLKEHNNYIEGRSY
                     LTITKEEAQELVNKHAGNGIIKFNRSGEWDKKELIEVDKNIGVNVNNITGEKTLTNKF
                     KIHYSKTGTHIVPAL"
     gene            1789..1989
                     /locus_tag="JD016_18765"
     CDS             1789..1989
                     /locus_tag="JD016_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001663.1"
                     /translation="MKLWEYVGKNVQITCVDKQIIRGKCDGYTQALDNEPEIASISIA
                     RDGYGIEVYENEIESIENINKE"
     gene            2055..>2528
                     /locus_tag="JD016_18770"
     CDS             2055..>2528
                     /locus_tag="JD016_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HAU5001664.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTITKEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKI"
BASE COUNT         1107 a          245 c          476 g          700 t
ORIGIN      
        1 acaggtgaca aatcgggtgt ggcacttaaa tttttatatt cattactgga ccttaaatgt
       61 tctaagactg aaaagaagtt taaaaaagca attagagagc ttttatggtt tgtgtgtgag
      121 tatttaaaga taagtggtaa taagagctat gattataaaa cagttcaaat tacttttaat
      181 cactctatga taataaatga agctgaaaag atagatatgg cagctaaatc aactggaatt
      241 gtatcagatg aaactattgt ttctaaccat ccttgggtcg aggatgttaa tgacgaactt
      301 gagagactta aaaaacagga agatactcaa aaagagtatg atgatttaat tcctaataat
      361 caagatggtg ttatagatga aacataaaga ttattggaga aagagatttg aacaattaga
      421 agaagctcaa aataacaaaa gtgtaaaata ttatcttgaa ttagaaaagc aatataaact
      481 agctatgaat agtatagaaa aagatatatt agcatggtac aacagatttg ccaaaaatga
      541 aggaatatct ttattagaag ctaagaaact actaaataca agagaactag aagagtttaa
      601 atggagtgtc gaagaatata ttagacatgg taaagaaaat gctataaatc aaaagtggat
      661 gaaagagtta gaaaatgcta gtgcaagagt tcatataaca agacttgaag ctttaaagtt
      721 acaaatacag caacaagtag aagttttata tggaaatgaa cttgatggta ttgataaact
      781 aatgagagat atttatacaa gtggatacta tcatacagct tttaatgttc aacaaggagt
      841 aaacgttggt tggagtttaa tgagtcttga tactaataga ataaataaaa ttatctctaa
      901 accatgggca acagatggat taaactttag tgaaagaatt tggggtaagt atagacctac
      961 tttagtaaat gaactacata ctaagctaac tcaatcaatt attagaggtg aaaatccaaa
     1021 aaatttagta aatgactttg ataaaagatt taatgtatct aagtcacaag ctaagaattt
     1081 gataatgact gaatcagctt tctttgcatc agcaagtaga aaagattgtt ttagtgattt
     1141 agatgtagag aaatatgaga ttattgctac attagattta agaacttcaa atatatgtag
     1201 agagctggat ggaaaaatat ttgatatgaa agattatcaa gttggaataa cagctccacc
     1261 atttcattgt cgttgtagga caacaacagc tccttggttc gaggatgaag aaggctatag
     1321 agcagcaaga ggagaagatg gaaaaacata ttatgtacca tctagtatga agtataatga
     1381 gtggtatgag aagtatgtta aacataatag tatcttagaa ataaaaaata gtgctataat
     1441 agatagcata aaagaagata ttaaaaatgg taaatataat ttaaatattc atgatgggaa
     1501 acaaggaaaa catttaaaag agcataataa ttatatagaa ggaagaagct atttaactat
     1561 aacaaaagaa gaagctcaag aacttgtgaa taagcatgct ggcaatggaa tcataaaatt
     1621 taatcgaagt ggagaatggg ataaaaaaga acttatagaa gttgataaga atataggggt
     1681 caatgttaat aatattacgg gtgaaaaaac tcttacaaat aagtttaaga tacattattc
     1741 caaaactgga acacatatag taccagcttt ataaggagga aaaataaaat gaaattatgg
     1801 gaatatgtag ggaaaaatgt tcaaataact tgtgtagata agcaaataat aagaggaaag
     1861 tgtgatggat atacacaagc tttagataat gaaccagaaa tagcaagtat atcaatagct
     1921 agggatggtt atggaattga agtttatgaa aatgaaatag aatctatcga aaatataaat
     1981 aaggaatgac taagcatgta cttaaaaata agtagatgct tttattttgt aaaaaatgaa
     2041 aggagaaatt taaaatggat tggttaaaag aattgctaga aggaataaaa gtagaggata
     2101 acaaaattga tgtagcttct cttcaaaaat ctatagaaaa gaaaataaaa gagactacaa
     2161 ttactaaaga agattataca aatcttgaaa cacaacttaa tacagctaat gaaactatta
     2221 aaaagtttga aggaggtatg acaaaagaag atgtagagaa tctaaaaaca acttatgaaa
     2281 ctgataagaa aactttggaa gaaacctaca aaaaagaaat tgaagaaaaa gattttaatt
     2341 actggttagg tgatgctttt aaatctgtta aatgtagaga tgaaatagca ttaaaagctc
     2401 atttagatat agaagcacta agaaatagta aagacagaca aaaagctttt gaagagcaaa
     2461 taaatccttt gaaacaggat aaagattatt tgtttaatgc aacactagaa ggtgaagaac
     2521 ctaaaata
//
LOCUS       DACXMZ010000340         2526 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.341, whole genome shotgun sequence.
ACCESSION   DACXMZ010000340 DACXMZ010000000
VERSION     DACXMZ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.341"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1265)
                     /locus_tag="JD016_18775"
     CDS             complement(<1..1265)
                     /locus_tag="JD016_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAU5001665.1"
                     /translation="MKERIKGAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKAEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGI"
     gene            complement(1265..>2526)
                     /locus_tag="JD016_18780"
     CDS             complement(1265..>2526)
                     /locus_tag="JD016_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAU5001666.1"
                     /translation="PASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDT
                     GRNVYLQPSLASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDP
                     KSERGNWKETLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTG
                     ISSRDGEKFPVLRKAVRSVTQSDSRGLLHVIDELRREDTPISRNIADHIDSFTDYDFA
                     HLLFSDGTVENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFAL
                     DFIHSDRSIFKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKE
                     SLKNNIGLKFAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVG
                     VVQIHPVFEELLHAFDTRPPVQRNEVE"
BASE COUNT          709 a          606 c          525 g          686 t
ORIGIN      
        1 tagataccag cgaagcatac tatctgcaag aacgccacca tgaaaaacgg ataatccgta
       61 gaaatgttat aaaacatact ggaaatgctg aacgccaccg ttacaataag cgtaattcct
      121 gcccgtgtca ttatggtatt aaacactctc acgattgcct gctttgccat gctttcatag
      181 ctcggtatca tggaaagtag aaaactgata ggtaaaaaca ttgcaaagat aataaaaagt
      241 atctggctga acaacatcat gcccgtaagc aagaatacaa atattgttat ccccaagttg
      301 aagaacagta ggaaaaacac catacccaag cggtttacca cctgcggtat tgtcagattg
      361 ttgttgtcgt tgtcctcgat ttctgctttt acgacttctt ctctggtttt cccgtcctcg
      421 tcctctgggc ttgccgatac cagagcttct acacggtctg tcccgatttc ctctgcattg
      481 ctgttaccga attgcagaag tagccacggc tgttccactt gaatagaaaa taagctgtcc
      541 cgtatcaagt ctacgctgtc cttgccctcg ctgtcagagt tagggagcat gatttttgtt
      601 cccaagtcaa gtgaagcggt actgatgtct gatgaaaact catttatctt cttgatgtag
      661 tcgggagcgt aggcaataaa ggaagcggac agcacgaaca ccaccacaaa gttgataaca
      721 gcgtgaagtg ccttgcttgt ttcccgtttt atcagtcccg tataagcaac atacattccc
      781 accactaaga taatgagaag caggaaaccg acataaaagc ccgtggaaga aaagccgttc
      841 tcggttacac ctgcaagggt ctgtatgctc tgcccgatac tgtctgccat atcgttaata
      901 aaatcaagtt tataggcttc ctgcaccaca tacccagttg cgttagataa gtaaaggctt
      961 atcgtccaga caaagttggt aatacagtaa agtccgtact gcaccgattt tccaatcccg
     1021 tccagccagt tccacggaag ccacgaccag ctattatcta cataaaagtc aagctggtag
     1081 ttggaaaggg gatattttga ataaagattt tctgcattta tggtatcgtc cacaagcccc
     1141 gtcgcatgag ccaccgtccc cagaagtgaa agcaggataa gggagagtgc caccacgaac
     1201 agagccattt tgagaaagtg gaaaatcttc ttttttgtga acgcaccttt tatcctttct
     1261 ttcatcactc cacctcattt ctctgtacgg gcggtctggt atcaaaggcg tgtagcagtt
     1321 cttcaaagac tgggtgtatc tgcaccacac cgacacgccc gtataagtcc tgcaataagc
     1381 attgtccgtt ctccaaatca cgaagccgtt tctggttgtt ttcgtcgtct ttgtcaatac
     1441 cgaaaaactc taaggtctgc tttatctcgt tgatgtcggt actacgaaag gcaaacttca
     1501 agccgatatt gtttttcaga ctttcctttg aaacatcata ggcggactgg gtaacaaaat
     1561 aaacgcctgc ctgcatagct cgtccagcac gaaccagctt atttgataag gtttcgcctt
     1621 gtgccacatt taagaacgcc cacgcttcgt caaggtctac aatcttaaaa atgcttctgt
     1681 ccgaatggat aaaatcaagg gcaaaggtac taatcacaat cagcatagac accgacaata
     1741 attcaatggt cgtgtattcc tcaaaagtgg tatctttatc tggcaacaca aggtctgcta
     1801 cttgaatgat attaagctga ttatccagac tgatagcatt ttctaccgta ccgtccgaaa
     1861 acagcaggtg tgcaaagtcg tagtccgtga agctgtcgat atggtctgca atatttcttg
     1921 atatgggcgt atcttcacgg cgtagctcgt ctatcacatg gagcaagccc cgactgtcgc
     1981 tctgggtaac ggaacgaacc gcttttcgta acacggggaa tttttcgccg tccctagagg
     2041 aaatacccgt aaggaatgtt aagatgtcga ttgccagact ttcagcgtct tttacattct
     2101 tcataatcac aaacgggtca agaagtcctg cattgtcctt atcgctggtt aggtttacga
     2161 tattgatttc atgggcgatt tctgggagcg tttctttcca gttgccacgc tcacttttag
     2221 ggtctaacag aagtgcttga ccgccaaaca gcaccgcata atataccaga agattattgc
     2281 agaacgactt accaccgcca agcgaaccga caaaagcaga agcaagggca tttgtaactg
     2341 tgccttttac gccctgcgaa gctaaagacg gttgcaggta cacatttctt cccgtatcaa
     2401 cggaatagcc catatagata cccgtagttt cccctaactg ctgtgtcgca ccaaaaccaa
     2461 gcccagctaa aaaatctgat tttacatact gcacatagtc attgatatat cgcttgctgg
     2521 ctggta
//
LOCUS       DACXMZ010000341         2504 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.342, whole genome shotgun sequence.
ACCESSION   DACXMZ010000341 DACXMZ010000000
VERSION     DACXMZ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.342"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            121..1635
                     /gene="glpK"
                     /locus_tag="JD016_18785"
     CDS             121..1635
                     /gene="glpK"
                     /locus_tag="JD016_18785"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAU5001667.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            complement(1749..2378)
                     /locus_tag="JD016_18790"
     CDS             complement(1749..2378)
                     /locus_tag="JD016_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU5001668.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
BASE COUNT          930 a          289 c          463 g          822 t
ORIGIN      
        1 cttcttacta aatatctatt atattaaatt tgtatttaag tatgattatt tattgaaata
       61 tgtgatatta aatttttctt tttaataaat taaatattat atgataagga gaatatggtg
      121 atggaaaaga agtatgtaat ggcattagac caaggaacaa ccagttcaag agctatacta
      181 tttaataaaa aaggcgaaat agtgaaaatt gcacaaaaag agttcaatca aatttatcca
      241 aaagctggat gggtagagca tgaccctatg gaaatatggg ggtcacaaag tggggtaatg
      301 agagaagtta tagaaacagc aggtataaga ccagaagaaa ttgcttcaat agggataact
      361 aatcaaagag agactacggt tgtttggagt aggtatactg gtaaacctat ttataacgca
      421 atagtatggc agtgtaggag aacgtcagaa atatgtgatg aacttaaaaa taaaggattg
      481 gaagaatcaa taaaagaaaa aacaggtctt ttaatagatg catacttttc agcaacaaaa
      541 gtaaaatgga tattagataa tgtagaagga gctagggaaa aagcagaaca aggagagtta
      601 ttatttggaa ctatagatac atggttaata tggaatttaa ctcgtggaaa agtacatgta
      661 acagattact caaatgcatc tagaacaatg atgtacaata taaatgattt agaatgggat
      721 gaggatatat tacgagaatt ggacatacca ataagcatgt tgccattagt aaaaccttct
      781 agctatgttt atggtcatac agatgagcgc atgttatcag gagctaaaat acctattgcg
      841 ggatgtgctg gagaccaaca agcagcatta tttggtcaaa attgtgttga agaaggtact
      901 gcaaaaaata cttatggaac gggttgtttc ttacttatga atacaggcag taacatagtt
      961 aaatctaagc atgggttact tacaacaatt gcttggggag ttgatggaaa agtaacatat
     1021 gcattagaag gaagtatatt tattggagga gcatctgttc aatggcttag agatgaatta
     1081 aagataattg aatctgcaaa agatagtgaa atgtatgcaa atagggtaga ggatactaat
     1141 ggtgtatatg tagtgccagc atttacaggt cttggagctc catattggga tatgtatgct
     1201 aggggttcaa ttttaggact tacaagagga gcaaaaaaag aacatatagt aagagccaca
     1261 ttggaatcaa ttgcttatca gacaaaggat gttttagaag caatgcaaaa cgattcaaaa
     1321 ttaaaactaa aatcattaaa agttgatggt ggagcaagta ataataattt tttaatgcaa
     1381 tttcaatcag atatattaaa tgtagatata gatagaccca aaatagtaga aactacagct
     1441 ttaggagctg cttatttagc agggttgtct gttggattct atattggaag aaatgaaatt
     1501 acttcaaagt ggtcagtaga aaaagagttt aatccaaata tgagtgaaga gaagagatgt
     1561 aaattatata aaggatggaa aaaagctgta tcgagagctt taagctggga aaaagaagat
     1621 gaattggata tttaaaaatt aatcaaagtt aaatttattt gaatttaaaa agagctttat
     1681 gtaaaatatg aagctctttt tttgttgtta atcttgcatt tttatataat cacactaaat
     1741 tatataattt atttttcgtt ttcatataaa aacattggta tagaattgta aatgtgttgg
     1801 tcaccaacta ctaagagaat gtctccttct aaaaattcaa tatatggtcc aggagatatt
     1861 ataagttctt cacctctttt aattgcaact attgtagcac ctgtattttg ccaaaattta
     1921 gtttgtgcag ctgtttgacc aatatatttg caattagaat ttatggtgaa ctgaacgggc
     1981 actaaaggat tgattatttc aagtctgtta gaatagtcta aaattttatt tatagtatct
     2041 aatatttcat tttctaaatt agatttagta tctattaaat tataaagtgt tgatttatat
     2101 gatgatatat ttgtaatatc tctatatttt tttataaaag aagaagcttt gtctggagat
     2161 agtacaataa cacctttacc ctttatgctt ttaacaacgc caatgtcctc taaaattttg
     2221 acagctcttc ttatcgtttc tggagataca ttgtatttac cagccaaaac agagcgtcca
     2281 aatagtactg aatcctcttg tatttcaccc gtgtatattt tatttgcaat atccaacgct
     2341 attttttggt atattggcat cgtatagttt ttatccattg tttttccccc aatacaaaat
     2401 ttatgttaaa aaaatatata atcttaacaa tactattgta taatttatag gtttatatgt
     2461 ctacaatatg tacaaatatt gtgcatgttt taaaataata tttt
//
LOCUS       DACXMZ010000342         2442 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.343, whole genome shotgun sequence.
ACCESSION   DACXMZ010000342 DACXMZ010000000
VERSION     DACXMZ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.343"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..887)
                     /locus_tag="JD016_18795"
     CDS             complement(<1..887)
                     /locus_tag="JD016_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAU5001669.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKKNYNI
                     SPTKYRNEHREVSNVILKE"
     gene            1029..>2442
                     /locus_tag="JD016_18800"
     CDS             1029..>2442
                     /locus_tag="JD016_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAU5001670.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINFPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVICISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYN"
BASE COUNT          831 a          341 c          303 g          967 t
ORIGIN      
        1 tcttctttta aaataacatt actaacttct ctatgttcat ttctatactt agttggagat
       61 atattataat tctttttaaa taagcgacta aaataattta tgctatcaaa gccacatctc
      121 atagcaattt ctgtcatatt taaatctgtt tcctttaaat aatatatagc tttatcaagc
      181 ctgattccat ttataaaatc gattgtagtc ttacctgtta tttgcttaaa tgttctacaa
      241 aagtgatgtg cactcaagtt cacacgacta gcaagttctt ttaatgtaat tttatccata
      301 tatttttctt ctatcaaatc aaagatacat tcaaattttt ctaaagaata ctttcttttt
      361 ataaattcat cttcagaata tattttatca atatgacttc ttaataagag tactattaat
      421 tgatagatat tagcttttat agataactca tatccaactt ctttattgtg atattcatca
      481 agtatagttg taatacattt taaaatcaga gaatcatttt gaattaaatt attaaataca
      541 atatgattta aagccaatgg tgctaagtac ttggtttgta acaaatcaac ttgattacta
      601 aaaaggaatg tgggttcaat acgaatacta tagaatttta aattatctga taagctttct
      661 aaataatgta attcattact gtttgctatt acaatatttc caggcactac cttaaaatta
      721 tccaatccac attcaagata tgctatacca tcaataaaat aatatatttg tagatgctca
      781 tgccaatgtc ttttaaagtt tgctcctttt ttaatgtcat cctttaaaaa aatatctata
      841 aagttttctt gacctttcct gttaaaatat gtatttttat ccttcattat tcccccctca
      901 tcaaaatagt gctaattata agcaatatag ttattgtatt cattatttca tatcgctatt
      961 atataaaatg taactatcat tgtaaacatt aacataatgc cagtcattat aataaggagg
     1021 taataaaaat gacaaactct tttaaaaaag atgataatag aaaaaaaatc acagttttat
     1081 ttgtagtcat tgccatgact tttatggcaa cattagattc tagtattata aatgttgctc
     1141 tcccagttct tgcaagcaaa ctaaatgtat ctctagcatc tattgaatgg gtaatagcaa
     1201 gttactcaat tattatttgt tcaacactat tattttttgg aagacttgga gatattattg
     1261 gtaaatcaag agtatttcaa gtaggtacta ttctctttac atcagcttca ttactatgtg
     1321 gattaagtaa ttctttaact ttacttatag tttgccgctt tattcaagga ataggcgctt
     1381 ctgcctacat ggcaaacaat catggaatta tcacagaact atttccaaaa gaaagcagag
     1441 gaaaagccct agggatttta gttactgcag ttgctattgg aaatatggtt ggaccatctg
     1501 tgggaggatt tattttgtca atatttgatt ggaatgttat attttttata aattttccaa
     1561 tcggtcttat tgtaatcttt ttaaatacaa aatttcttcc taatagtaaa aaatcttcag
     1621 aaaatatgga taaaactggt gctatacttc aatttttagg aacaacatta ttttttagtg
     1681 ctctaattag tgctcaacaa acaggattac taaatccata tatattaatt gcactattat
     1741 tatcaattat ttttattata ttatttttga tattagaaaa aaaacatcct caaccgcttc
     1801 ttgaccttga aatttttaga aattttaaat tttcattgaa tttaatttgt gctcttacat
     1861 cattcatttg tattgcatca tcaagtattt taattccatt ttatttacaa agtactatga
     1921 aacttccccc tattcaagca ggtttattta tgatactatc acctttgatt ttagccattt
     1981 tttcacctat atttgggaat atatcagata aaattaagtc agaaaaaatt attttaattg
     2041 gtcttctcgt tatgagtttt ggattctttc taatgtcaag attgaaagaa agttctgccc
     2101 ttattttatt tgtaatttgc atatcgataa tatcaatagg acaagctata tttcaacctg
     2161 ctaacaatgc tttgataatg tcaagctgtt ctagaagtaa gctaggagtt gtaggaagca
     2221 taaattccct tgtaagaaat ttaggacaag ttataggtat tactatttct acaacacttt
     2281 tatataattt tatgagtatt aaagctggat atagggtcaa tgattatgtg attaataatg
     2341 ataaaatatt tgtatttgga atgagaaatg tttatattat tgttacactt gtttgcttaa
     2401 taggtgcaat attaattggc ttttatttat ttaaatataa ta
//
LOCUS       DACXMZ010000343         2430 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.344, whole genome shotgun sequence.
ACCESSION   DACXMZ010000343 DACXMZ010000000
VERSION     DACXMZ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.344"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            309..2204
                     /locus_tag="JD016_18805"
     CDS             309..2204
                     /locus_tag="JD016_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HAU5001671.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     VDLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGRNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         1064 a          244 c          443 g          679 t
ORIGIN      
        1 atatatattt aagtaataag agtaaagcaa aaattagtgc atagtagtat ataaaaatgg
       61 agtttgaata cttttgttag tatatgattg tttagtagaa aatcactata aaataggatt
      121 tctatctttg agcataatta tagagagcta agtaggggga aggggttcaa gaagatatta
      181 ttagtgtttg tgttagtaaa ttatgtaaac acataaaata tattgaaaaa taatattata
      241 tataatatta tttatgtgtg ggaaatacga atataaaaaa taaaaaataa tattcggagg
      301 acataaatat gttaagcaac aaaaagagaa gtatggcaat agtaatggca ggtgctacag
      361 ttatgagtgc agcagcacca atattcgcag acaatactgt gacagaaaat gttgataaaa
      421 attacacagt aagtgcgaaa gattctgcta aactaatcga agaggttaga aaagcattag
      481 aagttaagtt tgaagataca aaagcaggag caaatgtaaa tgatagagtt tacgatataa
      541 aagtagacaa tgtaaactta actaatgcaa ctcaattaca aaataaaata aactctttaa
      601 cagaagggca aagtttaaaa gttactattc aagataaagg gcatcaagtg ttaggtggaa
      661 aagtagttga ctataaaatt gaaaactaca aaactgctca agaaatagta gatgcagtta
      721 atgcttacaa tgcaacttta gcagaagatt ctgataacaa gttaacagca actataaaat
      781 ctacaaatac agttgaagta aaaagagcaa aagattcagc aaatgttatt acattaaatg
      841 taggagacca acatttagat ttctcaaaag tgataacatc agaagaaggt acttttgaag
      901 gttatgaaaa gagttatagt gacatagatt caaaagaatt acatacgata acagttaaaa
      961 acgtagattt acaagacata tcagcagaag aattatttga tggaattaga ttaactacat
     1021 taggaagaga aatagttaat aaagttaaaa atggatatgc tttaacattt gaaaatgaag
     1081 caatccttac tcaagaacaa gaagatagtg atgataaaga taaaccagaa aaatcaagct
     1141 ttgatatagt tttaagcaag gcaaatgaaa agccagaaac aataagtgtt tcaagtaaaa
     1201 accataagtt agttagagac ttacacaaag tattaactga tgtaaaagat ggaaaagaat
     1261 taaaagtaga agttttatct ggtgattcaa gatttacaac agcagtagaa gttagtaaag
     1321 agagattcaa agatggagaa gcagaagcta taattttagt tggagaagat gctatagttg
     1381 atggattagc atcagcacca cttgcctctc aaaaaaatgc accaatatta ttatctaaaa
     1441 aagattcact accatcagaa atagaagctg aaatattaag agtacttgga agtaatctat
     1501 cttctaagaa aatatatata gtaggtggag aatctaaagt atcaaaagaa actgaagaaa
     1561 aactttctaa attaggtgta agtaaagttg agagagtttc tggagaagat agatttgaaa
     1621 cttctttaga aatagcaaaa caattaaaag atacatttaa gactgctttt gtagtaggtg
     1681 gaaatggaga agctgatgct atgagtatat cagctagagc tgctcaattt ggtgctccaa
     1741 taatagttac aggtaacgaa ttagatgcaa atgctgaaaa attattaaaa ggaaaagaat
     1801 tagaaatagt gggtggagaa aattctgtat caaaagaagt tgaagacaaa ttagttgata
     1861 tagatttaaa taataaagtt gaaagattag ctggagaaaa tagaaaagat actaatgcta
     1921 aagtaatcaa taaatactat gcaggtgcaa ctaaagcata tgtagcaaaa gatggttatg
     1981 taggtagaaa tggacaatta gttgatgcac ttacagcagc accacttgca gctagttcaa
     2041 aggctccaat agtattaact acagaagaac tttctaaatc acaagaagaa gtagttgagt
     2101 taagacttaa gaatgctact aagttagtac aaataggtga aggaatagct aaaaacgcta
     2161 ttgaaaaaat agcagaaaaa ataaacttat ttactaagaa ctaataagat ataggaatta
     2221 atattaaaag tttatttaga taatatatga ttatacaatg cgttaatatg taagacatag
     2281 tgtagatatt ataaaatata caaataacaa gataaaataa taaaaataag gtgtctcatt
     2341 tagaattaga ttgaggtacc ttattttatt ttttgattat aatgttgaga aattcatatt
     2401 gcaaaaatta ttataaaaag atacttctta
//
LOCUS       DACXMZ010000344         2404 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.345, whole genome shotgun sequence.
ACCESSION   DACXMZ010000344 DACXMZ010000000
VERSION     DACXMZ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.345"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(155..910)
                     /locus_tag="JD016_18810"
     CDS             complement(155..910)
                     /locus_tag="JD016_18810"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAU5001672.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1350..2339
                     /locus_tag="JD016_18815"
     CDS             1350..2339
                     /locus_tag="JD016_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAU5001673.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
BASE COUNT          873 a          302 c          359 g          870 t
ORIGIN      
        1 taccaataaa taagataatc tttaagaaaa atgattatat ttaaaaggta agcataaaat
       61 ttataaactt ttagaataga aatctcaatc aattagatat tcaaattgat gaatatgaat
      121 acctaaatag attgagattt attatgtaca aaacttatcc taatatatct gacattttat
      181 aaagcccaga ttgcttacca attaaaaatt tagctgcaac tatagaaccg tttgcaaata
      241 cttcatcaga ttgagaatga tggcttaatg taagaacttc attatcacct gcaaatataa
      301 catcatggtc gctgattata ttgccacctc ttatagagtg tattccaatt tcatttggtt
      361 ttcttttaca atctctacca tatctaccat gtgtattttc actttcaggc aagacttctc
      421 taattgcatt agctatcata gtagcagttc cactaggggc atcttctttt ctattgtgat
      481 gtttttctat gatttcaata tcaaaacctt ctaacatctt tgaagcatcc ttaactaatt
      541 ttattaatat atttactcca agagatgtat ttgatgataa taatatagga atgttttttg
      601 atgcttcttc tattttattt aagtcatttt cgtcatatcc agtagtagct aatactacag
      661 gtgtattatt ttttagagca tatgacaata ttggttctaa agtctcatga tgtgaaaagt
      721 ctataattac atcagttttt tcagttacat cagtcattgt tttgtaaagc tttgtatcac
      781 tatgatgacc tttttcatca gcagacctag ctacaccaca aaccaactct aattcattat
      841 ctttttttac acattctgct aaaactcttc ccattgttcc attatagcca ctaattataa
      901 ctttcaacat aaattcctcc tatgaaatta ataaaaattt gttatatttc tataagttta
      961 ttttatcact aatataattt atataaaata atattagtga taataagctt acaatttatc
     1021 ttacattaaa aaatttaagt ggatttttca aaatatagat aaatttcttt ttcattgtat
     1081 aaatgataaa aatatatttg actttatacc aaaaagagtg ccccatgaac taaaaagtta
     1141 attttgaaat accatattca tatatcatat gatttatttg gaaatcaata ttaatacatt
     1201 aaaataacca aatacatgta ttcataaaga tgtataaaaa atatataaac atatagtatt
     1261 aattttaaat tcaacaattt aattgttaaa agtgttatta ttaaattaga gatatcttaa
     1321 cttaatatac tgagatggga gtgatagata tgttaaaaga agaaaaaact tatcaagaga
     1381 aagacgcaaa agtattcaat ggagtggcag ctttgataat gattttatta tttttagtag
     1441 tttcaatagg tatcatggtt tttgggatag taaaacttga tagtggattt actggaatag
     1501 gttcacttat gttaattttg ggacttgtat ttatagtagt agattttatc ctattttttg
     1561 ggctaaaaat gattaatcct aaagaagcta tagttctagt tttgtttggt aactattatg
     1621 gaactataaa aaaagaaggg tactattggg tgaatccatt ttgttcagca ataaacccag
     1681 cagtatcaag aattaatgta tcaaaatctt catctgatga aaaggtagat atatcaacta
     1741 attcaagggg caaaaaagta tctttaaaaa caatgactct taataatgaa aagcagaaag
     1801 tcaatgattt attaggaaat ccaattataa taggagtggt tgtaatttgg aaggttatag
     1861 atgcaacaaa ggcagtattt aatgtggata attataatac attcttatct attcagtgtg
     1921 attctactat tcgtaatgtt tcaagacttt atccatatga tgtatctgaa gatggagatg
     1981 aaaaatctct tcgtggtagt agtcaggaaa tagcagatag attaaaagat gaattacaat
     2041 caagggttga tattgcagga attgaagtgt gtgaagtgag aattactcat ctttcttatg
     2101 caccagagat tgcagcagca atgttacaac gtcaacaagc agaggctata atagcagcac
     2161 gtaaaaagat tgttgagggg gctgtaagta tggtagagat ggcattgact aatttaagtg
     2221 aaaataatgt tgtgactttg gacgaagaac gtaaagcagc tatggtaagt aatctgttag
     2281 ttgtgctttg tggcaataaa gatgcacaac cagtggtaaa cagcagttct atttattaaa
     2341 ttataaaaag tttaatagtt tattttattg atatatagaa aatttagtag ttcattttat
     2401 taaa
//
LOCUS       DACXMZ010000345         2400 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.346, whole genome shotgun sequence.
ACCESSION   DACXMZ010000345 DACXMZ010000000
VERSION     DACXMZ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.346"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..402
                     /locus_tag="JD016_18820"
     CDS             <1..402
                     /locus_tag="JD016_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAU5001674.1"
                     /translation="VLFNTNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLV
                     PAQPKTTNETGRPNVKIEPKRVDSVNAPNAKTYTVKAGDSLWSICQKQLGNGSLYKKV
                     YELNKSMMDKANKGKKVPKYTIYKGQVLRLV"
     gene            395..2350
                     /locus_tag="JD016_18825"
     CDS             395..2350
                     /locus_tag="JD016_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HAU5001675.1"
                     /translation="MSDELVLANDRDVRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRIEENKTLFDMILTALDDTLRE
                     KKEMYTLYDDFGRITLKNVASMKLDTVMNNDVIEDLDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSAHMRSWGILQMFDTVDKNMSEVEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNANNKDEKKNGPVQSTTSKEDTDMANKINKLLKGKLSNTGNIFVKYSNAYK
                     VNPALMAAISMHESARGTSNIANTKNNFFGMKKNGDYMSFSSVDEGIKRGISNLSRNY
                     IHIGRKTLESIRNKYSSSSDKEWVKCVGAFYKQITGSTYNSNSAGTGVGSNEEAEKNL
                     KDVTYQIQGNNQSSSTNNSSKTDKLINVAKSKLGCKYVYGATGPNTFDCSGFTQWCYK
                     QIGIKIPRTVATQSKAGSAVDLKDRSKWKAGDLLCRVGGGSSNHVMMYIGNGQMIHSP
                     QTGDVVKIQSVDSYRKGKAYTHVRRFI"
BASE COUNT          984 a          277 c          465 g          674 t
ORIGIN      
        1 gtactattta acactaatat aaaagtatct cttgaagagt atgctatttc agaagacgca
       61 gataatggtt cagatacaaa agttgcaata aagttaaagc aatatagaga ttattcaact
      121 aaaaagttag ttcctgctca acctaaaaca actaatgaga ctggcagacc taatgtaaaa
      181 atagagccta aaagggtcga ttcagtcaat gcaccaaatg ctaaaacata cacagtcaag
      241 gcaggggata gtctttggtc aatttgccaa aaacagttag gtaatggctc actgtataag
      301 aaggtatatg agttaaataa atcaatgatg gataaggcta acaagggcaa aaaagtacct
      361 aaatatacca tctataaagg gcaggtgttg aggcttgtct gatgaattag ttttggcaaa
      421 tgatagggat gtaagattag tcatagcaca ttgggaagat ttctacgaac ctgtagtttt
      481 ggatggtatc acatgggaaa ttgaaaggcg aggaacacct tcaaagttag aatttacaat
      541 agtcatggat gatatattag agttttgcga aggtaactca gtaagattat attacaaagg
      601 tgtaggcata ttttatggat atatatttca aaagaaaaga gataaagaaa atcacataaa
      661 aattgttgct tacgaccagc taagatattt taagaataaa gatacttatg tatatagcaa
      721 taaaactgca agtgaacttg taaaaatgtt ggctaaagat tttaatttaa aatacaatgt
      781 catagaagat actaagtata aaatatctag gatagaagaa aataaaacac tctttgacat
      841 gatactaact gcacttgatg atactttaag agagaaaaaa gaaatgtata ccttgtatga
      901 tgattttgga agaataacat taaagaatgt tgcatcaatg aaattagata ctgttatgaa
      961 taatgatgtc atagaggacc ttgactataa ttcatcaata gatagtgata cttacacaaa
     1021 gattaaactt gtgagagata atgaagaaac aggaaaaagg gatgtatata ttgctcaaga
     1081 ctcagctcac atgagaagtt ggggaatact tcaaatgttt gatacagtag acaaaaacat
     1141 gagtgaagta gagataaaac aaaagtgtga tatacttcta aaactatata ataagaaaac
     1201 taagtcatta agtttaaaaa atgcacttgg agatattaga gtgagagcag gttgtttagt
     1261 acctgttttt ttaaatctag gagatattga attgcaaaat tatatgttag ttgagaaagt
     1321 aaaacataca tttgaaaata attcacattt catggatttg accttggttg atggagacga
     1381 atttgcttct tattcctctt caagctacag tagtggaaat gcaaataata aagatgaaaa
     1441 gaaaaatggt cctgtacaaa gtaccacaag taaagaagat actgatatgg ctaataagat
     1501 taataaacta cttaaaggta aattatcaaa tacaggaaat atatttgtta aatattcaaa
     1561 tgcttataaa gttaatccgg cactcatggc tgctatatct atgcacgaat cagctagagg
     1621 gacttcaaat attgcaaata ctaaaaataa tttctttgga atgaaaaaaa atggagatta
     1681 catgagtttt tctagtgtag acgaaggaat aaaaagaggt ataagtaatt tatcaagaaa
     1741 ctatatccat ataggacgaa aaactttaga aagcatcaga aataaatatt cttctagttc
     1801 agacaaagaa tgggtaaaat gtgtaggtgc attttataag caaataacag gaagtactta
     1861 taattctaat agtgcaggta caggagttgg aagtaacgaa gaagcagaaa agaatttaaa
     1921 agatgtaact tatcaaattc aaggcaacaa ccaaagcagt agtacaaata atagctctaa
     1981 aacagataaa ttaattaatg tagcaaaaag taaattaggt tgtaaatatg tgtatggagc
     2041 tacaggacct aatacttttg attgcagtgg atttactcaa tggtgttata aacaaatagg
     2101 tataaaaatt cctaggactg ttgcaacgca aagtaaggca ggcagtgcag tagatttaaa
     2161 agatagaagc aagtggaaag caggagactt attatgtaga gttggtggag gtagtagtaa
     2221 tcacgtaatg atgtatattg gaaatggtca aatgattcat tcaccacaga ctggtgatgt
     2281 agtaaaaata caatcagttg attcatatag aaaaggaaaa gcatatacac atgtgagaag
     2341 atttatataa gtgaggtgat aatatgagcc aagatttatt gcaaataata aaaaaagctg
//
LOCUS       DACXMZ010000346         2363 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.347, whole genome shotgun sequence.
ACCESSION   DACXMZ010000346 DACXMZ010000000
VERSION     DACXMZ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.347"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            79..2169
                     /locus_tag="JD016_18830"
     CDS             79..2169
                     /locus_tag="JD016_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001676.1"
                     /translation="MNKEYFSRKNTGGAINYIGTAFQDCVSLIYLFDNIDMEDYKEIT
                     FEQINDFTIIFKDYEVSVQVKSNYFDFKSVSTLLEKLEIKHGKKYVFVCSKYDKQFRA
                     FAIKLQQMKNVINSSRSNNEKEEIIEEIKNIAKKNGLNYEKAINVEFKELSLENRIDL
                     AKLKIYEWEKRNNLDIDVDALFDSLISNITQNLCTYRGFLSKNYINELALKYKSRKID
                     KNSTEYIKFVDNNVNSILEALKFDSSLYEMFTQQLNMTRFYISQNDFKNALKEIETIA
                     KYEQEFQKYKFFLLNINEDYKKLKNYCIEVLKNDESNYYANYYLGIEYKKIYKYKKAI
                     ACFLNAIDTMDTFDVNYNLAEVYEKSGQINNSLKYYNRCLEKDSMNIRANLNISKLLP
                     YNKAIEHLDIVISLDPEIYEAYLYKGKILRFLENIDEAFSNFQKYLKYREELEVIKEM
                     ALCMIDMNKKESNVFLSSWILESMQEELRELKDGESIIIYDILWNSSKVIICTKKEDD
                     FIVQSPISSFKLMNSNTNNLIVVGLVEDRFLKATSEFLRKNGRKAEKGKEYLPCFIKS
                     YDNKYDFDKFINSILEQKILHINKEYILNDEHGNEVEFREYISYESDVNIMMNDYNSH
                     RFVEVNIGSSTINGYFKKVGDGFFAFESKLEEGTPFNEGIIILECYEASKIIHIKMSL
                     DNIHSKKTLLYKYI"
BASE COUNT         1036 a          159 c          366 g          802 t
ORIGIN      
        1 gggatataag tttttataat aattaattga ataggaatta gttaaattta ttgttaataa
       61 ttaaggagga gtaaaaatat gaataaagaa tattttagta gaaaaaatac aggtggtgca
      121 attaattata taggaactgc atttcaagat tgtgtaagtc taatttattt atttgataat
      181 atagatatgg aagattataa agagataact tttgaacaaa taaatgattt tactattatt
      241 tttaaggatt atgaagtatc tgtacaggtg aagtcaaatt attttgattt taaatcagta
      301 agtacattat tagaaaagtt ggagataaaa catggtaaaa aatatgtatt tgtatgttct
      361 aaatatgata aacaatttcg agcatttgct ataaaattac aacaaatgaa aaatgttata
      421 aattcttcta gatcaaataa tgaaaaagaa gaaatcatag aagaaataaa aaatatagct
      481 aaaaaaaatg gattaaacta tgaaaaagct atcaatgtag agtttaaaga gttatcttta
      541 gaaaatagaa tagatttggc aaaactaaaa atatatgaat gggaaaaaag aaataaccta
      601 gatatagatg tagatgcttt atttgattca ttaatatcta atattacaca aaatctatgt
      661 acatatagag gttttttaag taaaaattat ataaatgaac tagcattaaa atataaatca
      721 agaaaaatag ataagaattc tacagaatat attaaatttg tagataataa tgtaaattca
      781 attttagaag cattaaaatt tgattctagc ttatatgaaa tgttcacaca acaattaaat
      841 atgactaggt tttatatatc tcaaaatgac tttaaaaatg cattgaaaga aatagaaact
      901 atagcaaaat atgaacaaga gtttcaaaag tataaatttt ttctattaaa tattaatgaa
      961 gattataaaa aattaaaaaa ctactgtata gaggttttaa aaaatgatga aagtaattat
     1021 tatgctaatt attacttagg aatagaatat aagaaaatat ataagtataa aaaggctata
     1081 gcatgttttt taaatgcaat agatactatg gacacatttg atgtaaatta taatcttgca
     1141 gaagtatatg aaaaaagtgg tcaaattaat aatagtttaa aatattataa taggtgttta
     1201 gagaaagata gcatgaatat aagagctaat ttaaatatta gtaaattgct tccatataat
     1261 aaagcgattg aacatttaga tatagtcata agtttagatc cagagattta tgaagcatat
     1321 ttatataaag gaaaaatttt gagattttta gaaaatatag atgaagcatt ttcaaatttt
     1381 caaaaatatc taaaatatag agaagaatta gaagtgatta aagaaatggc attatgtatg
     1441 atagacatga ataagaaaga atcaaatgta tttttaagtt catggatatt agaatcaatg
     1501 caagaagaac ttagagaact taaagatgga gaatctatta ttatttatga tattttatgg
     1561 aatagttcaa aagtgataat atgtacaaaa aaagaagacg atttcatcgt acagtcacct
     1621 atttcatcgt ttaaacttat gaatagtaat accaacaatt taattgtagt ggggcttgtt
     1681 gaggatcgtt ttcttaaagc tacatcagag tttttaagaa aaaatggtag aaaagcagaa
     1741 aaaggtaaag agtatctccc ttgttttatc aagagttatg ataataagta tgattttgat
     1801 aaatttataa attcaattct agaacaaaag atattacata taaataagga atatatattg
     1861 aatgatgaac atggaaatga agttgagttt agagaatata tatcctatga atcagatgta
     1921 aatataatga tgaatgatta taatagtcat agatttgtag aggttaatat aggaagttct
     1981 actataaatg gttattttaa aaaagtagga gatggatttt ttgcctttga aagtaaatta
     2041 gaagaaggga ctccatttaa tgaagggata attatacttg aatgttatga agcaagtaaa
     2101 attatacata taaaaatgag tctagataat atacatagta aaaaaacatt attatataaa
     2161 tatatttgat gtagtgggtt agggttttta ttgcaaataa aagtgtaata atattgtata
     2221 agcaaattaa atatatgtaa aaggtagtga tacaataaat attttatttt agtcaattat
     2281 ctttcaggat gtatttattg cttagagtta gttgatttaa gatttcttta agttatgtgt
     2341 taaaatgaga gaaaaagata aat
//
LOCUS       DACXMZ010000347         2353 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.348, whole genome shotgun sequence.
ACCESSION   DACXMZ010000347 DACXMZ010000000
VERSION     DACXMZ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.348"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(102..248)
                     /locus_tag="JD016_18835"
     CDS             complement(102..248)
                     /locus_tag="JD016_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HAU5001677.1"
                     /translation="MSPYIINSFQQNSCIKQYNFDNLNLSNKKESVYNDFIKVALRHF
                     LSYN"
     gene            251..2263
                     /locus_tag="JD016_18840"
     CDS             251..2263
                     /locus_tag="JD016_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAU5001678.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT         1044 a          226 c          341 g          742 t
ORIGIN      
        1 atttttttgg gtgtatatta taatttaatc gaatagaaat gaaaaatata catacagttt
       61 aagttttaag caacaattag ggggatttgg tgacaagatt tttaattata agaaagaaag
      121 tgtcttaatg caacttttat aaagtcatta tagacacttt cttttttatt tgataaattt
      181 aaattatcaa aattatattg ctttatacaa ctattttgtt ggaaactatt tattatataa
      241 ggagaaataa atgaaaaaat cagtagctct ggtaaatgat agtagaaaag atttaataga
      301 ctttttagaa aataatttga aattagtttt tggagattct ataaatatta atagatactt
      361 cataaatgaa ataaatgata atgatattat aaatgatgat gtgatattag taatgtcagt
      421 agaaagatta gacaaaatta taaataatat actagataag aaaaaagtga ttgtggtaag
      481 aagaactttt agggaagata aaatttataa tctactatct ttaccacagg gtacaaacgt
      541 tctaatcgta aatgattctg atgaaactac tctagaaact ataagtttat tttataaaat
      601 tggagtgact aatattagac caattccata tatgaatgat aataactata aaaatataaa
      661 aatagctata actccaggag ttccagaaaa agtacctagc tttatctcag atatatttga
      721 tttaggtcac agatatatag atatttctac atttatagaa ataataaatt tattacaaat
      781 tgattcaaag gaaatacaat ctaatctagt taaatactca gaagaaataa taagtttaga
      841 tactggtata aaagataaat ataaggaatt atttttaaaa atagaagaat tagatacaat
      901 attaaattta tcaaaagatg gtatattatt tacttcaaaa gatggtgaaa taaatacata
      961 taatagtaaa gtaaaagata ttttagacat aaatgaggat atatatggaa aatacattga
     1021 ggatatattt gtagatagtt taaaagtttt attgagtgag aaagagatac tagataaagt
     1081 tgtcgtattc aataagaaat atataaatgt aaataaaaaa aacatataca atagagatga
     1141 aaaaatgggc acatattata gtttacaaga aataacatac ataaaaaaat tagaacaaaa
     1201 tctcactaaa aaattaagag aaaaaggaca aatagcaaaa tatacattta aggatataaa
     1261 aacaaatagt cctaaaatgt ttgaatgtat agatttagct aagaaagttt ctaaatctga
     1321 tttaagcata cttatcagag gtgaaagtgg aactggtaaa gaactcatag ctcagtctat
     1381 acataacaac tcaaatagaa aaaatcaacc ctttatagca gtaaattgtg cagccgttcc
     1441 tgaaaattta ttagaaagcc agctattcgg atatgacaag ggaactttta ctggaggtct
     1501 aaaagatgga aaacaaggat tatttgaact tgctaataat ggaacaatat tcttagatga
     1561 aataggcgat atgcctttag aattacaaac aaaactactt agagttcttc aagagaagca
     1621 gataatgcct gttgggtctc acaatgtaat taatatagat gttagaatca tatctgctac
     1681 aaataagaat ttagaacaaa tgattgataa tagccaattt agagaggatt tgtattatcg
     1741 attaaataca attccaataa atatacctcc tctaagagaa agaaaagaag acatattaat
     1801 tattatggaa gatttaatta ataagaaatt agtaatcaca ccagaagcaa aaaaacttat
     1861 acaaaactat atgtggaaag gtaatataag agaactccaa aatgttacat cctatttaaa
     1921 cattatgtgt gaagatattg tattagagaa agatttacct ccaaacttaa ggtcttcaga
     1981 taacaaaaat acatcattaa aactaaaata tagtaagaat gatatattga atatattgga
     2041 gatactaatt ttaaataagg aatcagatgt tggtattggt aggggattaa ttttaaaagc
     2101 actactcgat aagaaccttc aaattacaga aggaaaaatt aaaaaaatat ttgaatatct
     2161 aaaaaaagaa gaacttataa tttgtagttc aggaagatat ggaagtaaaa taactcaaaa
     2221 aggagaagat ttttacaata aattaaaata taaaggtcta taaaattata atttatcaaa
     2281 caaagaaatc tattcaggat taaagtactc cttcaatagg tttttttatt tttcaagtac
     2341 cctaaaatta taa
//
LOCUS       DACXMZ010000348         2344 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.349, whole genome shotgun sequence.
ACCESSION   DACXMZ010000348 DACXMZ010000000
VERSION     DACXMZ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.349"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            265..1836
                     /locus_tag="JD016_18845"
                     /pseudo
     CDS             265..1836
                     /locus_tag="JD016_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein CwpV"
BASE COUNT         1027 a          312 c          413 g          592 t
ORIGIN      
        1 aaaaccctat tttttataac aattcattaa cataaaaatc aaacttacct taaaatataa
       61 atttacaaat ttttcaatca ataagaaaat ttctttattc cttacataat gactgcaatg
      121 taacaaaata attacaaatt aattatgaaa tcattatagt tagaaacctc tttaaaatta
      181 gatattaagt atcctttaga attagaacgg gaacaaagtt aaaaaattaa gtaatatcaa
      241 atggacaagg gggaagaaaa taaaatgaaa ataccaaaga agattgctgc aatgcttaca
      301 gttacaatga tagctggaag ttcaacagca ggcatagcta gtgcacaaac tgtggcaaca
      361 aatttaacag ggcaagaaag atatgaaact gctgttaaaa taagtcaaga tggatggaaa
      421 aatgctgatg aggtagtaat cgtaaacgac tcatctatag cagatgctct atctgcaaca
      481 ccatttgcaa aagcaaaaaa tgctccaata cttttaacaa gtaaagataa attaaatgat
      541 aagacgaaag cagaaataca aagattaaaa gctaaaaaag tatacttaat aggtggaact
      601 tctgtattaa gtacaaatat agaaaaagaa ataaaaaatt taaaaatatc ttttgagaga
      661 atctctggag ttgagagata tcaaacatcc ttagaattag caaagaaatt agatgctata
      721 agtgatgtta agaaaatagc tgtagttaat ggtgaaaaag gacttgcaga tgctgtaagt
      781 gtaggagcac cagctgcaca aaacaatatg ccagtaatac ttgctgattc taaaaatgga
      841 acagcagttg cagacaaatt tataaaagat gctggaataa ctcaatctta tgtagtaggt
      901 ggagagagtt caatttctga agctgttaaa aataaactac caaatagtac aagactaggt
      961 ggaacagata gaaatgatac aaatgcaaaa gtaataaaag aattttataa aaagacagat
     1021 ttaaaaaatg cttatgtaac taaagatggt atgaataaac aagaccaact tatagatgca
     1081 ctagcagtag gtgtattagg tgctaaaaat caatcaccag tagttttagt tggaaagaac
     1141 ttatcagctt cacaaaaatc tctagttaac tctaagagtt ttgataagat aactaaagtt
     1201 ggtgggaatg gtaatgagac agcatttaat gaaatgaagt cattacaaga agtaaaaaca
     1261 gttgaagcta aaacaatttc tgaattaaaa tcagctatag ataaagctac tgctaatgat
     1321 gttataaact ttaagccaac tagtgaggtt aaagaagcat ttacaataca aactgataaa
     1381 gcagtaacag taaacttaaa tggtacatat acaaagactg taactatcaa catgcctaat
     1441 ggagatgtaa ataactatgc aaaagtggat gatgtagtta tagatgatgt aaaagatgga
     1501 acttttgtta actatggaaa aataactaac ttaaaggtta atgataaaaa tggagccaag
     1561 atagaaaata attctaaagg tgagattggt agtttaacag tagcatctgg agcaagtcaa
     1621 gtaaaagtga ctaatggtgg taagataact acagttacaa ataattctaa agggactact
     1681 atagataata aaggtacaat aagtagtgtt aagggagata attctccaac aataagtgga
     1741 aatagtccaa gttctaattc atcaggagga agttcatcaa gtggaggttc ttcacatgga
     1801 ggaggctcat cttcaggagg aagttcttct aatcaaacat cagtaaataa tgaagcagca
     1861 aaaataacaa gcgtgcctac accagcaaaa gatgcaacta gattaactat gccatcagta
     1921 tcaagtggat atactatagc aataaaaaca tcaagtaatg agagtgttat aaaaaaagat
     1981 ggaacaataa tacctccaaa tacagcaaca acagtaaaat tagtatttac ggtaacacat
     2041 acatcaagtg gtaaaacagc agatacaaaa gaactatctg taacagtacc agctaaatca
     2101 acagatgaag aattagtaaa agcagaggca ttaaaaataa caagtgtaac tgcaccagca
     2161 aaagatgcaa ctaaattaac tatgccatca gtatcaagtg gatatagtat agcaataaaa
     2221 acatcaagtg atgaggatgt aataaagaca gatggaacaa tagtgccacc agatgcagaa
     2281 aaaacagtaa aactagtatt tacagtaaca catacagcaa gttcaaaaac agcagataca
     2341 gcag
//
LOCUS       DACXMZ010000349         2343 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.350, whole genome shotgun sequence.
ACCESSION   DACXMZ010000349 DACXMZ010000000
VERSION     DACXMZ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.350"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            80..2174
                     /locus_tag="JD016_18850"
                     /pseudo
     CDS             80..2174
                     /locus_tag="JD016_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT          937 a          237 c          392 g          777 t
ORIGIN      
        1 agttataagt aaatcttcta aaaagaataa ttttaacaat gttgttataa agagcagtaa
       61 ctatggagga gataagtata tgaacaaaca taattttgaa gttatattaa atcaactaca
      121 gattaatata tatgttacaa atattcatac taatgaaatt atttttatga acaaaaaaaa
      181 tgaaagaaga atataatatt ttagatccag aaggaaaagt ttgttggcaa gtattatatc
      241 ctgaaaaaaa ttctacttgt agtttttgta aagttttaga attattaaag aatgataaaa
      301 aaggagtatt aataaagtgg tatgaaaaat gtaataagtt aaatcgtgtt tttgaaaatt
      361 atgacagttt aattacatgg caagatggca cagttgttca tatgcatcaa tcaatagata
      421 ttgcaaactc aacaagctta aataaaccaa ttaagattaa tgaatttcat gaaatatcaa
      481 ataataaaga agaaaaagga gtttttaact tttcaagaga caattttgat tataattcta
      541 cattgcttta cgatgctttg attagaggta cagatgaata tatatacatt tgcaatatga
      601 aaacaggcgt atttcgatat tcaccatcac aagttgaatt atttgactta ccaggagaaa
      661 ttgttgaaaa tccgcttatt tactggaaaa aaattgtaca tccagaagac tggaatcgtt
      721 tttataagtc aaacactgaa ataggtaaaa accagatgga ttatcataca gtggaattta
      781 gggctaaaaa tcgtagtgga gaatatatat ggttaagatg tagaggtcaa ctaatgcgtg
      841 atgaatttgg agaaccaagt atttttgcag gaataatgac gcaattaggt aaacaaaata
      901 agattgattc attgacacag ttactgaatt atcatgaatt tatgagtgta tttgaagata
      961 aaatttcaaa tcctatgatt gaaaaattat gcatagtatt attagatatt gacgatttta
     1021 aaaatgttaa tgagatgtat gatagggatt ttggagataa tataataaaa acactagcac
     1081 agtctataca atctatttta ccagataatg ctgaacttta taaacttgat ggggacgaaa
     1141 tgggcattct tgttgataat gttgaagaaa atgaaattct aactctatat aatcaaatac
     1201 agaatatgat tatacacctt caactatgga gaaaatatgg attaaatata acaatttctg
     1261 ctggttgtgt aatatatcca aaacatggag atacagtaaa agaactttat aagtgtgcta
     1321 gttactcttt acaatatgcg aaagagcatg gtaaaaacag gcttgttttt ttctcccaag
     1381 aaatattaaa aaataaaatg tattcattag aaatgatgcg ggatttaaag gcatcaatta
     1441 atgatgattt tagggggttt agtcttcgat tccagccaca agtagataca gaaagtcata
     1501 aaataatagg tgtagaagtt cttttaaggt ggactaatga caaatgcaaa gcaatttctc
     1561 ctttggaatt tattccaata ttagaagaaa atgatatgat aaatattgta ggggcttggg
     1621 tgcttcgaat ggcattacgt acttttagga aatggattga ttactatcct ttttttaaag
     1681 tgagtgtaaa cgtttcagct gttcaaatat tagaagacac atttattgaa gatatagtaa
     1741 aaattataga tgatgaaaat tttccttatc aaaatcttgt tttagaattg acagaaagtc
     1801 atacagtaca aaatatgagt attttacagt ttaaatttaa ggctttacaa gatttaggaa
     1861 tttatattgc tatggatgat tttggtactg gatactcgtc tctagaagtt ttaaaatttt
     1921 ctccaataga tatagtgaaa attgatagag tatttgttaa agatatatta aagagtaagt
     1981 ttgatgctac atttatacat tttattgttg caatttgtca tgatgtagga ataaaagtat
     2041 gtttagaagg tgtagaaaca caagaagagt atgatttagt taaacaaata aagccagatt
     2101 acatacaagg atatttattt ggaaaaccgc agacagcaac agaaatcttt gaccttttaa
     2161 agctagataa ttaattctaa tttatcaata atatcactga aaagctttaa aatcaaaaat
     2221 acattaaatt attatattga agatatatca attataaatt gcaatataag aaaatagcat
     2281 ttcaatggat gttaaattgg gaatagtata tgattaaagt attttaaaaa gggataacca
     2341 taa
//
LOCUS       DACXMZ010000350         2276 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.351, whole genome shotgun sequence.
ACCESSION   DACXMZ010000350 DACXMZ010000000
VERSION     DACXMZ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.351"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(197..1489)
                     /gene="brnQ"
                     /locus_tag="JD016_18855"
     CDS             complement(197..1489)
                     /gene="brnQ"
                     /locus_tag="JD016_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAU5001679.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGIK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          763 a          376 c          247 g          890 t
ORIGIN      
        1 tttataatta taaatgaatc ttttttattt tattatcttt ataaatcata ctactccaaa
       61 aataaatttt taaatttatc aaataggatt aataatgttc ttttatctac aataatttaa
      121 taattctttt aaatataaaa aggaattgcc tatataattt aaaggcaatt ccataattat
      181 caattagttt ttattattat gcagttttac tgcttttaga tggaataaag tttcctatta
      241 ttcctgctat tacaactgga actatccagt taaatcctaa tgaagcaaat ggtaagctat
      301 taactagagg aatttttata ccaaaacttg ttgcaactgt taatacactt actaataatg
      361 ccatgtatgc agcacatttg aacacgttgt catttttaat tttattacta aataatgcta
      421 gtataactag agttatagtt gctggatata ccatacttag tataggagaa gagaattgta
      481 ttattgtact tactccaaag tttgatatga ttgcactaaa tacacaaact actataacta
      541 tatgctcata ctttaatttt ccatctgtaa gtgttgagaa atattgacca gctgctgaag
      601 aaagacctat agctgttgtt aaacatgcta atgcaactat aatagccaat aaaattttac
      661 ctgtagttcc aagtaaagct gctgttattg caactattaa agctgtttgt tgaacatctt
      721 gtccatattg ttgagacaca gttgctccaa gataacataa tccaccataa actaatgcca
      781 ttcctattac tgcaattaca cctgcactta ttgtggcttt catcttttct ttctcacctg
      841 tataaccttt agctattact gacatgatta taattgatgc aaatacacta gctgccaatg
      901 catccattgt ttgataacct tgagttatac cttctgcaaa tacattttgt attaaagaac
      961 tgtctcttat ttctcctaat ggagatacga tacctttaac tattaatact gctaaagcta
     1021 taagtaaagc tggagttaat attgacccaa ctatatctac aacttttgat ggtcttattg
     1081 ttaaaaccaa tactagagca aagaatacta tagagaatat tactgggctt attccagaac
     1141 ctattgtagg taatataccc atttcataag ttgttgctgc tgttctaggt attgctaaaa
     1201 gtggccctat acagattacc attgcacatc ctaatgcaat acttaatcct ttgcctgctc
     1261 ttgagaacat cttcgatgtg tctccattga acttagttgc tgctataact gctaataatg
     1321 ccaaaccacc atctgcaaaa aggaatccta tgaaagctac tagccaacta cttccagata
     1381 aaactcctag atatggaggg aagattaagt tccctgcacc aaaaaacatt gcaaatagtg
     1441 caaatccaat tacaattaca tctttaaaat tgatttgttt ctcacccatt taaagcaccc
     1501 ccatttgaat tttactacct tttactaaaa aataagtcca atctcttaat taaatgaaaa
     1561 ccttttgacg agattttatt taattataca tttttgactt aaatttgtca attatgtttc
     1621 ataatttata aaatcttcac aatatttaat acaactttct taattaaaca aaatcaacct
     1681 gtatacttta aacatagttg gttttttgtt ttttctctaa tttttcttga taaaattaac
     1741 tattccgaat aattaaaata ttaagacttt ttagattatt cgatttatta ttaagttgta
     1801 ttgctttata tactgtatat atcactctcc ttttttacat tcatataatc tttttaattc
     1861 aatttaaatt ttaaatattt tgtgtttatt atataatctg aaaaaaaagc agtcaatagc
     1921 atttttaatt tttttaattt tcaaaattat gattcttaaa aaatcgtttt ccttttatca
     1981 taacacctat ttacgttaat tcttgtagta ttttgtattt attttgctaa attgacagtt
     2041 aactttattc aatttttatt ctacttgcgt agatttgcct cttgtttttt actcttaaaa
     2101 ccattttttc tgaaaattcc ttattatgat ttatcaactt atttttcttc ataacaatta
     2161 ctctctaact tccaacttac ttaaactttg cagttgctag tattcacgtt tacatactta
     2221 tatgtcataa ttaactaaaa attcttaata tatctctcaa tgaaaacaat ttactc
//
LOCUS       DACXMZ010000351         2240 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.352, whole genome shotgun sequence.
ACCESSION   DACXMZ010000351 DACXMZ010000000
VERSION     DACXMZ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.352"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..618
                     /locus_tag="JD016_18860"
     CDS             <1..618
                     /locus_tag="JD016_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAU5001680.1"
                     /translation="KFQTVLYKKSDADYEGIVSIENKIKDKDLVESSLIYWVTGAIAG
                     CDINKSNTNKKYDGEFDIDVNYTQIQLEEALKSGKFIFHKVGDEVHVLEDINTFVSFT
                     DDKNDDFSSNQSIRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELEN
                     IRAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            635..1105
                     /locus_tag="JD016_18865"
     CDS             635..1105
                     /locus_tag="JD016_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001681.1"
                     /translation="MAQTINAKDTVSAKKAECFVTIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            1177..1617
                     /locus_tag="JD016_18870"
     CDS             1177..1617
                     /locus_tag="JD016_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAU5001682.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELRVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            1617..1811
                     /locus_tag="JD016_18875"
     CDS             1617..1811
                     /locus_tag="JD016_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001683.1"
                     /translation="MRGGDFDASITHYCIQKLKWKPSEYMNLEVNERALAAASILVKI
                     EDEEEAMKEAERERKRGRRR"
     gene            1958..2119
                     /locus_tag="JD016_18880"
     CDS             1958..2119
                     /locus_tag="JD016_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001684.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
BASE COUNT          909 a          216 c          443 g          672 t
ORIGIN      
        1 aagttccaaa cagtactata taagaaaagt gatgcagatt atgaaggtat agtatctata
       61 gaaaataaga ttaaagataa agatttagtt gaatctagct tgatttattg ggtaactgga
      121 gctatagctg gatgtgacat aaataaatct aatactaata aaaagtatga tggtgagttt
      181 gatattgatg ttaactatac tcaaatacaa cttgaagaag cactaaagag tggtaaattt
      241 atatttcata aagttggtga tgaagttcat gtattagagg atataaatac ttttgtatca
      301 tttacagatg ataaaaatga tgatttttca agtaatcaaa gtattagagt acttgaccag
      361 attgctaatg atattgcaac tttatttaat gaaaagtatt taggtaaagt tccgaatgat
      421 aaggcaggaa gaataagttt ctggaatgat gttgttaaac accataaaga attagaaaac
      481 attagagcaa tagaggattt caaaactgat gatgttagtg tggagcttgg aaacgataag
      541 aaaactgtca tagtatctga tgctgttaag gtaataaatg ctatgagtaa actgtatatg
      601 acagtttcag taagctagaa aggagagtgt tagaatggct caaacaataa atgctaaaga
      661 tacagttagt gcaaagaaag ctgaatgttt tgtaactata gaaggcaaaa gatataactt
      721 tatgcaagct atagatttag aggctaaaat ggaaaaaaat aaaagtgaag ttccaattct
      781 aggaagaaca acaaagggaa ataaaacaac tgggagtaca aatactggaa gtgcaacatt
      841 tcattataat acttctattt ttagagaatt actttacaga tataaagaaa ctggtgagga
      901 tatttatttt gacatacaag ttacaaatga agaccctaca tctgctgtag gaagacagac
      961 agtagtactt aaagattgta atatggacag tggaataatt actaaatttg atgctgatgg
     1021 tgagtattta gatgaagata tggatttcac ttttgaggat tgggaattag tagaaaaatt
     1081 taatttattg gcaggaatgg agtaaaagac acatttataa attatatatg tgtatttttt
     1141 atatgaaaaa ttaaaataaa aggagattag aataatatga gtaatttaag tgctttttta
     1201 agtcaaaatg caataaaggt tgataatgta aaatatgtag cgagtaacag atttttagat
     1261 aaagaaggga aaccagttga atgggaatta agagttttat catctgaaga agacgaagca
     1321 ctaagaagaa aatgtactaa aagagtaaaa gtgattggta acaatggtaa gcatactgga
     1381 caatatacaa gtgaaattga ctacaacagt tatgtagctg aattatgtgt agcatctaca
     1441 gtatttccag atttaaagga tgccgaactc caaaatagtt atggagtaat gggagaagct
     1501 cagttattaa agacaatgct tacagcaggt gagtatgtca attatacagt aaaagtgaat
     1561 gaagtcaatg gatttgatac atcttttgag gataaagtag aagaagcaaa aaactaatca
     1621 gaggtggcga ttttgatgct agcatcactc attattgtat tcaaaaatta aagtggaagc
     1681 caagtgaata tatgaattta gaagttaatg agagagcgtt agcagccgcc tcaatacttg
     1741 taaagataga agatgaagag gaagcaatga aagaagctga aagagagaga aagaggggac
     1801 gaagaagata gcaaaataaa aaaataaata tagaataggt aaaatatgta ataattatat
     1861 gttataatat ttttagcaag aagatgtaat ctacaattta tagagtggag ttcatactgg
     1921 gataaaacct acttcctaat gaaaggaggt gggaagtatg aataactttt tacttaatgt
     1981 aatagctggc gttattgcta gtttaatatt ttgcttaatt tgtaaagtat ttctaaaagt
     2041 aaaaagccac tcaactcgtg gcaagagtaa aagtggctgg gaatttgatt ttaaaatcaa
     2101 gttccataag ttcaaataga ttcatttaat tatgaacttc actctaccgc aaaatagatt
     2161 gtagttcttc ttgcttttat tataccacaa attagaaaaa atattgttta tataaaataa
     2221 aaaataaaaa tttttattaa
//
LOCUS       DACXMZ010000352         2167 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.353, whole genome shotgun sequence.
ACCESSION   DACXMZ010000352 DACXMZ010000000
VERSION     DACXMZ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.353"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..282
                     /locus_tag="JD016_18885"
     CDS             <1..282
                     /locus_tag="JD016_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAU5001685.1"
                     /translation="LEKLIRNQNIKIDYITLSHIYETKCKEGLNPKGIELLKEAKKIT
                     DIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIKKTISNYNEKLN"
     gene            470..1864
                     /gene="yfcC"
                     /locus_tag="JD016_18890"
     CDS             470..1864
                     /gene="yfcC"
                     /locus_tag="JD016_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAU5001686.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
BASE COUNT          769 a          259 c          363 g          776 t
ORIGIN      
        1 ttagaaaaat taataagaaa tcaaaatata aaaatagatt acataacatt atcacatata
       61 tatgaaacta aatgtaaaga aggattaaat ccaaaaggaa ttgagctttt aaaagaagct
      121 aaaaagatta ctgatattaa aataattgca cttggaggaa ttttaccatc taatgtcaaa
      181 gaaacattaa aatactgtga tgattttgct ataatgtcaa ctataatgag gtctaaagat
      241 attaaaaaaa ccatatcaaa ttataatgaa aagttgaatt agtaaatata gtatattgaa
      301 tttttaactt aaataaaaat aaaaaagtta tcttttaaat tttaaaaggt agctttttta
      361 ttttatatct ttgctaataa aaaattgtaa aaagggaaaa ctttagtcag agagattaaa
      421 caatatccta gaaagattta aaatttagta aagtatggag gacattataa tgactgacag
      481 taaaaagaaa cgatttatta taccacacac ttttacaata atatttttac taatagtctt
      541 aatggcaata ttgacttgga tagtaccaag tggtgaattt agtagagaag atgtagaagg
      601 tagaatggta gttgtaccag ggacttatca aagtgtagaa agtaatcctc aaggatttgc
      661 agatgttttt aaagcaccaa tagaaggctt tatcgattca gctgaagttg taggttttgt
      721 tttaatagtt ggaggtgctt ttggtattgt aaataggact ggagctatag aagcaggaat
      781 agcttcggtt atctctaagt ttaagggaaa agaacttctt ataattccaa tatcaatgat
      841 tttatttgga cttggaggta caacatttgg gatggcagaa gagacattac cattttatat
      901 gatatttgtt ccactcatga catccttggg atatgattct ctaacagcaa tagcaattgt
      961 atttatagga gcagcagcag gagcagccgc ttcaacaata aatccatttt cagttggtat
     1021 tgcacaggca ctagctcaac ttgctcctgg ttcaggagta acttacagag tagttatttt
     1081 tatagcatat atactaataa gtatagcatt tgttatgtac tatgctaaga aggtaaagga
     1141 aaatcctgaa agttcaatag tatatgaaat agataaaaat aataaatcaa tacttaatca
     1201 aacttcaaca gaaataaaag aatttacttt taaagaagta tctgttcttg ctatatttgg
     1261 aatagggatg gcaataatga tttggggtgt tttaagtcaa ggatggtaca ttccagaaat
     1321 atcaatgtgt tttgctgcaa taggtgtgct ttctggattg gttggtagat tatctcaaat
     1381 tgaaatctca gaaagtttta tagatggagc aaaagattta gcatctgcag ctcttgctat
     1441 agctttggct cgtggaatcg taataattgc tcaaaatgga tttataatag atactatatt
     1501 aaattccgca gcagcctttc taggtgggct tccaaaggtt atatttgtat atttatcatt
     1561 tttcttggaa gctgcattgg catttttaat tccatcatct tcaggtcttg cttcacttac
     1621 agtacctgta cttgctcctc ttggtgattt ggttaatata tcggctcaag tgatagtaac
     1681 cgcttatcaa tttggtgtgg gtcttacaaa ctttataact ccaacttcag gagtactgat
     1741 gggagcactt gcagtagccc atattccatt ttcaaaatgg gtaagattta taatgcctat
     1801 cttgttgatt ttgacagctg tatctctagt ctttttaaca ataggtattt atatagggtt
     1861 ttaattaagt gtagttttat ttaagatatt tagtaatggt ataaaatgat aatatgtttt
     1921 caataaattg ccactaattt aattagtggt aatttgtttt ttgtatttaa aaataaatgt
     1981 atttacttgt attttgaatt aatatctact tagacattgt tttaattgtt atattttcat
     2041 ttaattaagc attgatttat cattttttat atttcatata acaatgcaaa aaataatttt
     2101 tatatagtaa atgacaaaag tttacaataa atgtatattt tcttactcat tccttaaaat
     2161 tactcat
//
LOCUS       DACXMZ010000353         2125 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.354, whole genome shotgun sequence.
ACCESSION   DACXMZ010000353 DACXMZ010000000
VERSION     DACXMZ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.354"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(39..1394)
                     /locus_tag="JD016_18895"
     CDS             complement(39..1394)
                     /locus_tag="JD016_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAU5001687.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT          770 a          379 c          238 g          738 t
ORIGIN      
        1 agtagattaa cctataaaac ataataattg cttactaatt atttatcatc ctcaacaagt
       61 ctacttaatg tcttatcaga taagaataat aatatcgctg caacaaatag tattactgga
      121 attatagtat atactttaat catatcaaat ttaagagtaa atgcatatat atatccatat
      181 cctttaccag ctataaaagt agcaaatgtc catactccca taagaacacc tagaagcttc
      241 ttaggtgcat acttacttac aaaagagtta ccaagtggtg agaataacat ttcaccaaga
      301 cttaaaagaa taccaaacat gattatccat agtatactag ctttagcagt ttcaggagct
      361 ccaactcctc ttgaaaattc tgcaccaaca agcattaaga atgcaagacc taagaatatt
      421 aatccaagac ctgtcttttt aaataaactc atatctcctt gtggtctact agcaagttta
      481 aaccataatg ctccaagaac tggtcctaat actatacata caagtgaatt taatgagtca
      541 aaccaagcaa gaggaacatc aaatcctcca acattcatgt ttacaaatgc tgcaccataa
      601 tcataaactg caagataagt tagataccag aatacccaga aaattacaga gaagaatgat
      661 ataagtataa tagcccaaat tctcttctta tccatgcttg taagaggaga cttatctttt
      721 tcttcaattt tttcagcaac tacaccttct ttaaaaggtc tctttcctgc attcccaagg
      781 aatctccatc catatatgaa ccatattgca cctattacac aaagtacagc tgctataaag
      841 aaacattgag aaaatccaag tacaccattt tttgcaaatg ttttaaggta aagaatacca
      901 actgctatag taccaataaa agaacctata ttaacaaaag aatactgtac tgaaaatgct
      961 gtatcaagtt cttcttgact atcaaaaagc tgtccattta ccgcagatac gttaccttta
     1021 aagaatgcag taccaattga aactaagatt atcattgcat ttaccataga aactgtagtt
     1081 gcaatagaac caagatagta tcctactccc atgataagca tacctatagg tatagtatat
     1141 ctagctccta tccatctatc tgatatatat cccccaatta taggtgcaag gtatgtgaat
     1201 gcaacaaggt tggcttgcat tatagcagct tgtcctttat ctattccaag accaccatga
     1261 actatagctg ttgtaaggaa tatatatatt aagtactttg aagcataata tgctgctctt
     1321 tcaaatgaga aagttgttga gcagatataa aatcccatag gaaatttacc cttctttttt
     1381 actgttgagc tcataatttt aaatcctttc ttaattgtat ttttattttt tacgttttgt
     1441 ccaaattctt tcttttatga aagatataat attattattt tgtaaaacaa tttgtaatta
     1501 ttattaagat aattgcgcat ttatttgtag agtacatatt ctcatggaat aaaattaata
     1561 cacttcttaa tcataaattt aaattatata aattcactat tttcagatat agttataaat
     1621 taatatattc tcatctaata ccatgaaaaa accttcgcca tatgcaattc ttatttttta
     1681 cgtgatagta ttttacaaac taaaatcccc cttttgtagc aacttatgta attacatttt
     1741 agtgcttttt taaaggagtc tcgaataaag caacatatat tttgttcaaa taatgttttt
     1801 atttattttt taaatttata tattacttca cccaaccaat tctcaactta aataactaaa
     1861 ctactatcta ttcacagatt aggcttattt cttacacatt agtttataat aaacactgtg
     1921 aatctaaaca taaaaactgt tattttaaag taattaccca tatattcgct ttgatactat
     1981 ttattgaggt tatactcttt atgtataata tattatttat aaaatacctc aaaatatatt
     2041 attcataaaa tacctcaaac tgaaaacttt ttcacgaata tttatttttc acctcccctt
     2101 ttttatttca ccatgtaaat cgtac
//
LOCUS       DACXMZ010000354         2099 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.355, whole genome shotgun sequence.
ACCESSION   DACXMZ010000354 DACXMZ010000000
VERSION     DACXMZ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.355"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            58..2082
                     /locus_tag="JD016_18900"
     CDS             58..2082
                     /locus_tag="JD016_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAU5001688.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT          889 a          249 c          302 g          659 t
ORIGIN      
        1 cttgccttat actttaaata ctttaaaaat aaatactatt ttgttaggag aaataaaatg
       61 gataaaaaga agttaatcct tataacatat gataaattga atagtgacca ctacaaagaa
      121 gaattaacaa acttttttgg agacgaaata atcatagaaa cacaaaatat acttgatgga
      181 ataaaagaaa atttagaggg agatgtagtg ctttctctat cacctttaac tagtaatttt
      241 ttaataaaac attttaagga agatattgaa attattcatg gaacgaaagc actttcaaaa
      301 cttggatatg aaaaaatgat gaaactgcct ccaggtacaa aatcgttact tatgacaacc
      361 aataaaactt cagcatttga gatggctact tatctatata aaattggtat aaatcatata
      421 gattttgtac cgacatatcc agattgtgat gaaatatatg atttagatac agctataaca
      481 ccaggtcaga ttagatttat accaaaatac ataaaaaata ttgttgattt aggttggaga
      541 aaaatctcat tagatacata tatgtcatta ttagtagtat taaaattaaa aaatgagaaa
      601 tttatagaaa aactatacaa attgtccaag gagacactaa gtcatgattt cttaaataca
      661 tcattagata atatatctaa attaaaaact atactttaca tgaccattga tgaaataggt
      721 gatggtctaa tatttttcaa cacctttaac aaggttactt ttgtaaataa gtctttactt
      781 aatatgttgg aactagatga aaagcttata aaatcacctt ccttaatgga atatatgcca
      841 aagagtttct tagataaaat tacaaagaac ttaaatatcg ataatatgat tatatacata
      901 gacgaaatag ataaaaaatt catactttct aaaaaaccat tttatcttta taaaaatata
      961 gagggttgct taattacatt aaaagatgta aacaatatag aaatattaga acaaaaaata
     1021 agaagtgatt ctgtcaaaag aggttatgta gctaaatata aatttaataa tattattgga
     1081 aatagttcta ttataaaaga ttgtattaaa aaggcaaaaa aaatggctct tacagataat
     1141 cctatattaa taacaggtga aacaggaaca ggtaaagaag cctttactca gtcaatacac
     1201 aatcattcaa atagaaaaaa taaacctttt gtagcaataa attgtgcttc attaccaagt
     1261 gaattacttg aaagtgaact atttggatat gaagatggtt cttttactgg agcaaaaaaa
     1321 ggtggaaaaa aaggattatt cgaattagct cacactggca ctatattttt agatgagata
     1381 ggagatatgc cacatgattt acaggttaaa cttttaagag tcttacaaga aaaagaaatt
     1441 agaaaaattg gtggtacaag tattattcca attgatgtga gaatattagc tgccacaaat
     1501 aaagatttag agaaattaat tatagaaaat aaatttagga tggatttatt ttataggata
     1561 agtatgttca ctttagattt acctcctctt agaaagagat tagaagatat acctcttctt
     1621 ttagaatcat ttttaaaaga acttccctat aaaaacataa aactcgataa gagtctttta
     1681 gaagctttaa attcatacac ttggatgggg aatattagag aactgagaaa ttgtgttgaa
     1741 tatatggctt acatgggttc caattatctt acaataaatg atttacctca aaacatatct
     1801 tcaaaattaa ataataatca tatgtcaagt aatatgtcca tattcaatga tttaaaccaa
     1861 tatgacaaaa acatatgcat ttctatccta aaatcattgc acatgaaacc aatggggagg
     1921 acaaagttaa tgaaatttat ggaatataat gtcacagaat acgaagtaag aaatatgcta
     1981 gaatatttga cacgcaatgg atacttaatt tctagtaagg gaagaaaagg ctcatcaata
     2041 actgagaaag gcataaaaat aatagaaaat aatataattt aaacctatag taattcatc
//
LOCUS       DACXMZ010000355         2087 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.356, whole genome shotgun sequence.
ACCESSION   DACXMZ010000355 DACXMZ010000000
VERSION     DACXMZ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.356"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(19..438)
                     /locus_tag="JD016_18905"
     CDS             complement(19..438)
                     /locus_tag="JD016_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5001689.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            737..946
                     /locus_tag="JD016_18910"
     CDS             737..946
                     /locus_tag="JD016_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5001690.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            966..1187
                     /locus_tag="JD016_18915"
     CDS             966..1187
                     /locus_tag="JD016_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001691.1"
                     /translation="MNILVCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKY
                     SSTCSILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(1404..1802)
                     /locus_tag="JD016_18920"
     CDS             complement(1404..1802)
                     /locus_tag="JD016_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU5001692.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
BASE COUNT          773 a          245 c          268 g          801 t
ORIGIN      
        1 tcaaaagcaa cataaaaatt atttatctat tttctcatct ttatttttgt tatactcaat
       61 ttttcctaaa tccttctctt tttcatattc atgaagtttt aattcaatca taccttctat
      121 tttagcttta tcataatcat ttaattttct aaaattgttt aaaagcttta tttcattaga
      181 gtttatgcta ttaagtggat agtttgagga ggaatcgcaa attaaatctg atttatgtga
      241 tagattatct ccatttaata gccagtctac tgaaacatta aatatctcag ctatagattt
      301 taatatttca taatttggtt ttctaatgtt tctctcaaat ttacttaagt tgtcacagcc
      361 caacatttct tctagttcat attgtttaag gttttttgct tttctcaaat aaacaattct
      421 ttctcctaaa gtatccataa acactctcca ttcaattaat gtcaaaaaga ctttttaaga
      481 tgtaaatagt ttcaaattaa aggtcaaaat gacataaaaa ccattgactt aaggtcaaaa
      541 tgactttata attaacttaa tgatacgaat ttacatccta attttagcac aaagtaatca
      601 aaaaatctta tttagtatta aataaattta tatacttaat atgtgtacat attaaaaata
      661 tatactaaat agagggggtg cgtaagctaa aataatataa aagtaaatat aaatcgctta
      721 gaaaggaagt tgataaatgg atgctcgaaa aaaatggata ccttttttgg gagtgcaagt
      781 caagcaaaga cttattgaat taaatatgac tcaaagggaa ttagcgaaga aaataggtgt
      841 taatgaaaac tatttgtcag ctattttaaa tggaagaaga acaggtaaaa aatataaatc
      901 atcaatttat caattactta atatagaata ttcagaagat gattaataaa tagtatataa
      961 agtaggtgaa tattcttgtg tgcaaattgg attcagatgg ggttatagag tgttgtagag
     1021 caattgatga ttttattaca gcactcagta atataaaaag cttaaatatg gaaagattaa
     1081 atactttaac taaatattct agtacatgtt caatccttct taaagagggg aattatgaag
     1141 gatgtacaat tgtgtataga aagatgttgg aagaattaaa aacatgagta atgcatttct
     1201 taggaatcta agttatacat agagatgtat tatatttttc aaagtactta aactaaaata
     1261 tggataagat aatctaaata ttataaatgt gcttgaaatt agactatact tgtttttaaa
     1321 taatccaata tccatatttt agtaatatac tacaaaaaaa gaaggttaat agatgatgta
     1381 aaatcgtatc aaattatgta tgtttaaacc attttatctt cattattatt agaggaatgc
     1441 ttttttaagt ctttatattc agatatctta agttcaagta ttccttctat ttttatttta
     1501 tcacgttcgt ttagttgtct gtatagattt aatatcgtca tttcatcatt agtaacatgt
     1561 aagtaatctt ctttatcttc ttttacacta ctattgacat ttaccttctc tttaccatag
     1621 agaagccagt cagtcgtaac attaaaataa tcagctattg acattagtat atcacaatta
     1681 ggttttctat ctcctgtttc atacttgcct aagttttcaa attttaaaat atccataagt
     1741 ttgcgctgag taagtttttt ggagtttctc aaataagcaa ttctttttcc taaagtatcc
     1801 acaaaataca ctcctttctt ttatgagtaa tgtctaaatg acatttaaaa ttaaaaatat
     1861 ataaatttat aatataaaac tactaaatta aagtctaaat gacattttgc ttaaattaat
     1921 atgctcataa tatgatttta acatattata gttgaaaata tatggtttat tttgatttgt
     1981 atatataaca ataggtttaa ttgttataaa aatgtaaagg ggtgtatgaa tagattgtat
     2041 aaatttattt cgataaacta agattgcttt ttgattgtct gtaaaag
//
LOCUS       DACXMZ010000356         2066 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.357, whole genome shotgun sequence.
ACCESSION   DACXMZ010000356 DACXMZ010000000
VERSION     DACXMZ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.357"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            176..976
                     /locus_tag="JD016_18925"
     CDS             176..976
                     /locus_tag="JD016_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAU5001693.1"
                     /translation="MELERPLLYDEVENKDDKNNNRKFNIKNKIFKGIGILFVLILLG
                     FFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKGRKVSKGLILYPGAKVEIES
                     YAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSYNNIEHWVIGGHSLGGVAAS
                     NFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKDGVVNFKSLVESKQKLPDDT
                     TYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANSIIKFLKNIS"
     gene            1085..1942
                     /locus_tag="JD016_18930"
     CDS             1085..1942
                     /locus_tag="JD016_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAU5001694.1"
                     /translation="MPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIKPLEV
                     IKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYGSPKS
                     IEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKSGIKL
                     SATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGEFTIL
                     EDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYLSNQK
                     LKREEYRRL"
BASE COUNT          856 a          201 c          370 g          639 t
ORIGIN      
        1 catatattta tgaattttat atgaaaatag ttaaaatatt gatttttttc attggttgaa
       61 aaaacaattt acttaggata aactataata gtattcaaat attatggggg ttagatttat
      121 gagtaaagat gtaaaggtaa agcgtaataa gaaatatgat acatttagta taactatgga
      181 acttgagaga cctttacttt atgatgaggt tgagaacaaa gatgataaaa ataataatag
      241 aaaatttaac ataaagaata agatatttaa gggaataggt atactatttg tattgatttt
      301 gcttggcttt ttcatatgga taaataaaac atatgaacct caaaagctgg caaaagaagc
      361 tctagtcagc aatagtaaag ttgaagtaac tgtaaatgaa aacatttcat ttacaccaaa
      421 aggaagaaaa gtttctaaag gtttaatttt gtatccagga gctaaagtag aaatagaatc
      481 ttatgctcca cttgcaagaa aaatagcaga aagtggatat gaggttgtaa tcgtaaagat
      541 gccacttaat ctagctatac ttggaactaa taaggcacag aaagttatgg attcttacaa
      601 caacatagaa cattgggtaa taggaggtca ttcattagga ggagttgcag catcaaattt
      661 tgcacgtgat aataaattaa ttgaaggcgt tgtattttta gcttcttatc caatgggtga
      721 tgagttgaaa gaattaggaa aaaaagttat atctatatgg gggagcaaag atggagtagt
      781 caattttaaa agtcttgtag aatcaaaaca aaaacttcct gatgatacaa cttatgttga
      841 gattgaagga ggaaatcatg cacaatttgg agactatgga aaacaaaagg gagataatga
      901 tgctattata agtcaagaaa aacaacttaa tattactgcc aatagtatca ttaaattttt
      961 aaagaatata tcttaaacta aaatttaaaa aatataattt tataagttat tatttaaagt
     1021 aaatattaat aatataatag aatgaatatt ttattacaag ggggatttta atatggaata
     1081 cgatatgcca ctttacagac caccaagtga ggcttatagt cttataatac aagtcacact
     1141 aggatgttct cataacaaat gtacgttttg tagtatgtac aaatctaaaa aatttaaaat
     1201 aaagccacta gaagtaataa aaaatgaaat tgatatattt agaagatatt ataagcatgt
     1261 agaaagaata ttcttagcag atggagatgc attgataata ccaatggaga aattaagaga
     1321 tattatttta tatataaaag aagtttttcc agagtgtgaa agaattacta tgtacggttc
     1381 tcctaaatct atagagaaaa aatctgatga tgaacttaaa gaattaagaa aacttggtgt
     1441 taaaatgatt tatctagggc ttgaaagtgg aagcaatgaa gtattagaag atattaagaa
     1501 aggttttagt agtgaacaat taataaaagt tggtagaaaa gttaaaaaaa gtgggattaa
     1561 attatcagcc actgtaatag caggtcttgg aggaactaaa aaaacacatc agcatgcagt
     1621 tgatacagga aaaatgttag gagcaatttc accagattat gtgggtgtac ttagtttaat
     1681 ggtagaacct aatactgaac tttacgattt acttaaggca ggagagttta caatactaga
     1741 ggataaggct gtgttacaag aaattaaaga gatgataaag aatatagatg ctaatgaaaa
     1801 aattgtattt agaagtaatc atgcatcaaa ttatgctaat ttaaaaggtg tattacctga
     1861 tgataaacaa cgtctaattg atgaaattga ttactatcta agtaaccaaa agctaaaaag
     1921 agaagaatat agaagactat agtttttgat atatcaatct atattgatag gtaaatacta
     1981 agattattta gatacaaaaa atcatgctac attaatagtt gcttactatt gatgtagcat
     2041 gattttttta ttaaaaaaca aaatgg
//
LOCUS       DACXMZ010000357         2040 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.358, whole genome shotgun sequence.
ACCESSION   DACXMZ010000357 DACXMZ010000000
VERSION     DACXMZ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.358"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..434)
                     /locus_tag="JD016_18935"
     CDS             complement(<1..434)
                     /locus_tag="JD016_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001695.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPHSEDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNF"
     gene            complement(427..852)
                     /locus_tag="JD016_18940"
     CDS             complement(427..852)
                     /locus_tag="JD016_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAU5001696.1"
                     /translation="MARWGSVDFREFKRVCKKMEELTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            complement(852..1199)
                     /locus_tag="JD016_18945"
     CDS             complement(852..1199)
                     /locus_tag="JD016_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001697.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQLIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(1193..1594)
                     /locus_tag="JD016_18950"
     CDS             complement(1193..1594)
                     /locus_tag="JD016_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001698.1"
                     /translation="MENNLIDEIEKRLESFGYILKDGDKWLIDFIREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNNILTFRCLRW"
     gene            complement(1604..1837)
                     /locus_tag="JD016_18955"
     CDS             complement(1604..1837)
                     /locus_tag="JD016_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001699.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYSKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(1837..>2040)
                     /locus_tag="JD016_18960"
     CDS             complement(1837..>2040)
                     /locus_tag="JD016_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HAU5001700.1"
                     /translation="AGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGV"
BASE COUNT          609 a          403 c          203 g          825 t
ORIGIN      
        1 tcaaaattat cacctctctt caaaccaccg aataactcta ctgtatccat cttatctgtg
       61 tcattacttt ttatagtaaa gtagttataa tcaacaaaga agtgaagaac attgtctata
      121 atttcaaaat tcatatttgt acctctgact aaatctccat caatttctat atactctaat
      181 tcctccagta gcatctcagc tatctcattt atttcaaaat tcttatcttc tgaatgaggg
      241 aaataatgta catcaaaaga atttttcttt aattctctcc cgcttggata tggtgtcttg
      301 cttggattta aaggaacaat aaaaaaacaa ggttcattaa taccttgctc cacatcttcg
      361 ctataaattg tatatttttc tccaaacgat ttatctaact ttactgatat tccatctata
      421 atattattaa gcatcaaaca ctcctttaag caatattaat agttttttct ctattatctt
      481 atcaacttga ctttgtagtt ccatttctga aatagttaag aaatgttgtc ctttaaccca
      541 tcccttacca tctttagttc tatgcccgaa atttacataa cttgcatatt cagtcggatt
      601 aacaacttct ataatataat tatttccttg tttatacaca ggaagcgacc tagcataagc
      661 cactccattc catccttgtc gtaagaatcc tgtatcaact ggtgttcttc taattacttt
      721 cccaagtaat cgtgctgcta attctcttgc tgcatccttg caaaacttat ctaaatcaat
      781 ctttgtaagc tcctccatct ttttacaaac tcttttaaat tctctaaaat caacactgcc
      841 ccatctagcc attatgcttt atccttaaat aactcaagta ttatttcttg atgatttgga
      901 tatatagctg attctccact tcttacatac tctttatcat ttataataag ttttgaacct
      961 gctttaattt ctatatctgg agatataaag agtttaatag tttgctctag cttagctact
     1021 ttcccttctg tagcagaaac tatattttta tatgaaagtt tacatggttg attttctaat
     1081 acaatcactt ctttattgtt agttcgtttt gttacagggt ctttaattag ctgatactca
     1141 actatagtac atttatctct atataacatt tctattgctt ttctagtttt acttaccatc
     1201 ttaagcacct aaaggttaat atattattct taccataagt agtaagataa gctattaagc
     1261 tatcaaagcg ttgttctggt gtttgagaac cacttcctat agcaaaatct acctttgtat
     1321 caccttctga tatagacttt tctacagctt caaagttaat gctttctata tctaattgac
     1381 ccatattttt cttggtaaat aagaactctc caactatcat atcaacttca atttctttca
     1441 attcaattgg catagttttt atattacaat ctagtttaat aatattttct attttttctc
     1501 ttataaaatc tattaaccac ttatctccat cttttaatat atatccaaaa ctttcaagtc
     1561 ttttttctat ttcatcaatc agattatttt ccataatttt cacctacttt ttagtaagct
     1621 tatttttctc tttaagctgc ttattttctt cttctaaaga ctcaactttt gaccttaaaa
     1681 tattattttc agctattaaa tcttttacat ttaatgactt gctatacttt actgccttac
     1741 cagtttcatc tatcaaatca tatcccatct ctaagaaatc atctatttta cactcttcta
     1801 tagttaatat tctatttaat ttccttactt gtgccattat actccagctc cttcaacaac
     1861 aaattgtatt gcatcagctt ttttatttaa tataaataca tcctcaaaac tttcttcaaa
     1921 gtagaagtat tttccctctg taactgctgt tggttcgtct aacttagaga actgataaga
     1981 aacaggtgta attattgcac ttgggtgaac taaggacata aatatttgtt tagctcctgc
//
LOCUS       DACXMZ010000358         2024 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.359, whole genome shotgun sequence.
ACCESSION   DACXMZ010000358 DACXMZ010000000
VERSION     DACXMZ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.359"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..384
                     /locus_tag="JD016_18965"
     CDS             <1..384
                     /locus_tag="JD016_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAU5001701.1"
                     /translation="EHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNIKINLNI
                     KGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIERGIVECTGVFGNMKSI
                     YLKKTNGNLIELSNHRITESKPWKI"
     gene            372..1310
                     /locus_tag="JD016_18970"
     CDS             372..1310
                     /locus_tag="JD016_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAU5001702.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSILTVFLIIFILKQNNLI"
     gene            complement(1694..1918)
                     /gene="secG"
                     /locus_tag="JD016_18975"
     CDS             complement(1694..1918)
                     /gene="secG"
                     /locus_tag="JD016_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAU5001703.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
BASE COUNT          735 a          272 c          252 g          765 t
ORIGIN      
        1 gaacatgtgg tcattactaa taataatata gaaaattata tcaattttta taaaaaattt
       61 ggtttttcat ctaaagaaat gagcaaaaat tataaactta atgctagaaa tattaagata
      121 aatcttaaca taaaaggtta taaattttta ccttatgtta aaaattcaaa agcaagtaga
      181 gataacttat gttttaaaat aaacacaaat attgatgagc caaaaaaata cttagaacaa
      241 aatgagattc aaattgagcg aggaattgta gaatgtacag gtgtctttgg taatatgaaa
      301 tctatttatt tgaaaaaaac taatggaaat ttaattgaat tatcaaatca tagaatcacg
      361 gagagtaaac cttggaaaat ttaatcctat caataaatgt tgttcttcct ttgtttctca
      421 caatgtcact tggatatgtt ctaaaaaaat taggtctatt tgatactcat actttaaata
      481 aaatgaataa tgtctgcttc aagtctttct tgccattatt attatttttt aatatataca
      541 atacagattt agatggttct cttaatttta agcttatggc tgtagctgtt ataattattt
      601 tttcaatctt cttaatctta gtctttatta ttcctaaaat tgaaaaagat aataagacaa
      661 ggggtgcatt aatacaaggt gtatttcgta gcaatttcgt tatatttggt attcctgtga
      721 caatttcact atttggcgat aagagtattg gagtcacatc tttattaatt gcagttattg
      781 tacctatgtt taatgtatta tctgttgtaa ttcttgaaat ttttagaggt ggcaaagtaa
      841 atataaaaca tatgattaaa agcgtcttaa caaatccact cataatcgcc tcttttatag
      901 gattgacaat gctaatttta aatattaata tacctacatt tttacacaaa actattggtg
      961 atatttctaa aatagctact cctttatcac ttataattct aggcggctct tttagttttt
     1021 ctgctataaa ggggcatata aaacccatta tattgggcgt atgtggaaaa cttatacttg
     1081 tacctgctat atttttacca attagcattt ttcttggatt tagaaatgta gaattagctt
     1141 cacttatgat aatgttatct gctccaactg cagtttcttc atttacaatg gcacaacaga
     1201 tggattctga cagtgaatta gctggacaaa ttgtagtatt tacttctgga ttttctatac
     1261 ttacagtatt tttaataata tttattttaa agcagaataa tcttatttaa tgatatttga
     1321 taacacaaat tggtattatg agaaacatat ataaaattga tttaactgcg taaaaacata
     1381 ttattatact atttttacta tataatataa ctttgtgtat attatctcat taaacatggt
     1441 gaaagtagtg ttgctatata ttttttagtt tgaagaaaat atataaatac aaacaatata
     1501 taaatacaaa taataaacgc caacaaatag tcaatctata aaataaaaac tggactaatc
     1561 atataaaatc attagtccag cttttatttt acgtactttt tatgttgatg tacttttatt
     1621 aaaaactaaa tttaattttt aaatgtctag caaatgcact aattaaataa tggccagtta
     1681 ttcaacaata tcactacttc ttaactataa gtaatgccaa tgcatttata aaaaataata
     1741 ctgctgttat ctttgtaact ctagcaagaa atgcttgttt tccttttggt ttaaagtaag
     1801 cttgacttcc ttctccacca aattctgaag gtaaagcatt cttagaatct tgaggcatta
     1861 tgctaattac tagtaaaatc cctagtacta cttgcacacc cattaaaatg ttgctcattc
     1921 ttgcccctcc tatctgttta ttacttaaat ttcattactc tttagtgtat cacaataata
     1981 tagccatttc aagactatta tgctataaat aacatacaaa aaaa
//
LOCUS       DACXMZ010000359         1994 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.360, whole genome shotgun sequence.
ACCESSION   DACXMZ010000359 DACXMZ010000000
VERSION     DACXMZ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.360"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            124..1437
                     /locus_tag="JD016_18980"
     CDS             124..1437
                     /locus_tag="JD016_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAU5001704.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            1455..1991
                     /locus_tag="JD016_18985"
     CDS             1455..1991
                     /locus_tag="JD016_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAU5001705.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
BASE COUNT          763 a          200 c          397 g          634 t
ORIGIN      
        1 tatgtagtta gttggatttg tgtatttaaa taatgtataa tttatgctga ttactgctaa
       61 aaaagcataa taatattgca ttgtaaggtt ttataaactt ctaattgaaa ggggataatt
      121 caaatggata agagatattt agaatccgat gtatttgaag cttttcaaga aagactttct
      181 tatcttttta atgaatttga ttatatctat gtatctttta gcggaggaaa agatagtggt
      241 ttactgctaa atttagtttt ggattataag agaaagaata atataagttc caaaatagga
      301 gtatttcatc aagattttga ggctcaatat agtttgacta ctgaatatgt tgaaaaagta
      361 tttgaagata atattgaaga tattgaacca tattgggtgt gtttaccaat ggctacaaga
      421 actgctgtag gaaattatga aatgtattgg tatccatggg atgatgagaa agaagagtta
      481 tgggtaaggg aaatgccagt caaagaatac ataataaatc aaaaaaataa tccttttact
      541 tattataaat ataaaatgag acaagaaagt ttggcaaaac aatttggtag atggattaga
      601 gatatacatg aaggtgcaaa agttgtttgc ttattaggaa cacgagcagc agaatctcta
      661 caacgttatt gttctattgt gaataaacag catggatata aaggaaagtg ctggataact
      721 aaggaattta aaaatgtctg gacggcatct cctatatatg attgggaaat agaagatata
      781 tggttagcac actataaatt tgactatgac tataataaaa tttatgactt attctataaa
      841 gctggagtag ctccaagaaa aatgagagtg gcatcacctt ttaatgattc agcaaaagag
      901 agtttaaatt tatacagaat attggaccca gatgtatggg tgaaattggt tgctagagta
      961 caaggagtta attttacttc tatttatggc aaaacaaaag ctatgggata tcgtagtgtt
     1021 aaattgccat atggacacac atggaaaagt ttcacatatt ttttgcttga taccttacct
     1081 gaaaatataa ggaatagcta tattaaaaaa ttttcaacat ctattgagtt ttggcagaat
     1141 acaggtggtg gtttgtcaga aatagttata cgtgaattgt tgagaaaagg ttataaaata
     1201 gaattaaatg gagtttcaaa tcatacaaaa gataaaaaga gtagagtcat atttttggat
     1261 gatatacctg atgatacgga tgatattaaa tttaccaaag atataccaag ttggaaaaga
     1321 atgtgttatt gtattttaaa aaatgaccat atgtgtagat ttatgggatt tggtcctaca
     1381 aaagaacaga gaattagaac ggaatatatt aagaagaaat atgaaaaaat tcattagata
     1441 ggagttaaga tgctatggaa tataaaagtc ctgtatatgg aatcatcgca gtgccaatcg
     1501 aaaagataga atctaacgac tataatccaa atgcagtagc cccaccagaa atgcagttgt
     1561 tgtataaaag tataaaagaa gatgggtata caatgccaat tgtctgttat tataatgagg
     1621 aaaaggataa gtatattatt gtagatggtt ttcatagata tagagtaatg cttgaaaatg
     1681 aggatatata tgatagagaa aatggacttc ttcctgtttc agtaattgat aaatctcttg
     1741 atgaaagaat ggcaagtact attcgacaca atcgtgcaag aggttctcat aatgtggatt
     1801 taatgagtaa aattataact gaactttgtg aaattggaaa atctgatgaa tggatatcaa
     1861 agcacctggg tatggaagtt gatgaaattc ttagattgaa gcaaataact ggattagctt
     1921 atttatttaa agataaagag tttggtaatg catggattcc ctttggctct gatatagaag
     1981 aagaaaatta aata
//
LOCUS       DACXMZ010000360         1977 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.361, whole genome shotgun sequence.
ACCESSION   DACXMZ010000360 DACXMZ010000000
VERSION     DACXMZ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.361"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(49..1266)
                     /locus_tag="JD016_18990"
     CDS             complement(49..1266)
                     /locus_tag="JD016_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAU5001706.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(1457..1870)
                     /locus_tag="JD016_18995"
     CDS             complement(1457..1870)
                     /locus_tag="JD016_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU5001707.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT          675 a          325 c          225 g          752 t
ORIGIN      
        1 aagtgatttt ttagattata tattttaata tactttctta tataagaatt attttaaatc
       61 aagattatct agtattttta aaaatatttt tacagcagta tccaagtcat ctacagaaac
      121 actttcatta ggattatgac ttacgccccc agcgcatctt ataaatacca taccaatatc
      181 agttatatta tccatttcct gagcatcatg gccagcacca ctataaatat aaaaaggatt
      241 aagatttaaa tctataaaac ttttttctat gatttttgtt attttattag aacatggtac
      301 aggaacattt tcaaaagcaa gttcacttgt ataattaagt tttctatttt cacatatatg
      361 tgatatttca ttaaatatct tttctacaga attatcaaga atttcttgtg aacaagagcg
      421 tacatcaaga gtaaattcag ctctttcagg taccacattt gatgaagatg gatagaaatt
      481 catctttcct acagtagcaa ccaaatcagc agtcgtttta gctacttttt cgatagctaa
      541 tacacattca caggcacaac atccagcatc aagtctcata ttcattggaa gtgtaccagc
      601 atgtccagac ttaccattta ctgatatctt aaaactttta aatcctgtaa tacttgatac
      661 aattccagca ggaagagatt cattttctaa aacaggaccc tgctcaatat gaaattctaa
      721 atatgcatct acatcttttc taggatgcaa atttgttaag ttttttgtat taaatccaaa
      781 ctttgttaca gcttcttcaa gtgtaattcc attttcatca acagatttaa gaagttcctc
      841 tttaaaagta ccagcaaaaa ctttacttcc cattaaagaa gtttcaaatc tagttccttc
      901 ctcttcagcg aaagcaatta gcttaatatt gaaaggataa gacctattat tgtgttttgc
      961 ttcttcaaga ccaactagag gcactattat acctaacata ccatcaaata tacctccaca
     1021 ctttacacta tcttgatgag atgcaactac taatgtggga gcatttggaa agcttgattt
     1081 atatgtacct ataacgtttc caatgtcatc aatttcaata tcaagattta aaccttccat
     1141 ccatgaacta attaaatcaa tagatttttt atgttcttga gtcaagataa gtcttgtaag
     1201 accatttgtt gttgaactta tttttcctaa tgtttgtaga taatccatac atttttttcc
     1261 taacatattt acctcctaaa aatataattt ttatttaagc taagattagc ttttatactt
     1321 ataatttttg aactttcaaa taattttgta tgtatagtaa aatactattt tagtattaat
     1381 aatgatataa aaacataaag tctgtagacg aagagcagaa gttttaattt aaaaatatga
     1441 aatatgattt ttattattat agtatttttc tataataata tctctttcca gttagaggat
     1501 aattttcaag tgaaaatact ttatggtatc caaatttctc ataaaattta ggtgcttgaa
     1561 aactgaatgt atctaaaaaa acatatttac aacctcgttc tttagcaatt ttttcagcct
     1621 tatataaaag ttgacttcca gtacctttat atctaagctt ctcactaacc cataagtatt
     1681 ttatagaaag ccaattacca tgagtaacac ctataaaacc tgcaacaatt ttacctgctt
     1741 tatctcgaat atatattcct aaatccactg ggtttttatc ttcaagtcta gcaagattat
     1801 attttaataa tccttgaaaa atagtatctt catcttgttt ttctattttt tctgtaattt
     1861 ctaattccat atttgaactt tctccttgta taatcttttg tatattggta ttttttaata
     1921 aaatcagtag aaagaatata gtttaacatt attctaatag gcatttatgc taatgtc
//
LOCUS       DACXMZ010000361         1973 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.362, whole genome shotgun sequence.
ACCESSION   DACXMZ010000361 DACXMZ010000000
VERSION     DACXMZ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.362"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            97..1008
                     /locus_tag="JD016_19000"
     CDS             97..1008
                     /locus_tag="JD016_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HAU5001708.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1183..1680
                     /locus_tag="JD016_19005"
     CDS             1183..1680
                     /locus_tag="JD016_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAU5001709.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPADKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
BASE COUNT          818 a          143 c          350 g          662 t
ORIGIN      
        1 aataatttag ataagattct aagattttgt ttatttaaaa attagtatga ttaatttaga
       61 ttaaataaat ttaataattt tagtaggtgg taatgaatga aaataaatag attaacagaa
      121 ataatagtaa ttctattaaa taaaaaatta gtaacagcta aagagttggc agatagattt
      181 gaagtttcta caagaaccat atatagagat atagaaactc tgtctatgtc gggagtacca
      241 gtatatatga cgaaaggcaa aggaggaggg atatctttaa tagaagaata ttctatagac
      301 aaggccatat tgtcaaaaaa agataaagag agtttaatag tggcactgaa aacattacag
      361 gcgactaagt atccagaaat aaattcagta gtaaataaaa ttggctcaat atttggagag
      421 cagaattttt caaactggat tgaaatagac tttactgaat ggggaagtaa ttttaatgaa
      481 gatgataaat ttacaaaaat aaaagaagct attttaaggc gtaatactat aaattttaat
      541 tatgtaaata gtttgtcaag ccagacaaat agaactgtcg aaccactgaa gcttatgtat
      601 aagagtaaga cttggtactt gtatggattt tgtaaattaa aagatgattt taggatattt
      661 agaattagta ggataaggaa tttatctata aaggatgaag tgttttcaag aaagataata
      721 gaagaagtat gtttgaatga ttctaaagta attaaggaaa atactataac gttgaaattg
      781 aggtttaaag aaaagatgct ttttagagta tttgatgatt ttaataagga tttaataact
      841 aaaaatgaag atgatacata tgatgtaatt acagaatttc ctattggtga atggatttat
      901 ggatatattt tatcatttgg tgacaatgtt gaagtacttg aacctaaaga tgttagggat
      961 aatgtaataa ctagactaag ggagttgtct aaaatttatt cattataata taatgctaga
     1021 ttaaagattt tattaaatat ataatatctt gcatttagta tataatatct cactgagttt
     1081 aattatatta aaaatatgac ataaaggtgt cgtatattat aaattataat tgaattagtt
     1141 caaaataaaa tagattaata aaaacaaagt gaggtataaa gtatgaatta tgaaatagta
     1201 gagataaatg agaaaatagt tataggaata tcgaaggaga ctacaaataa agatggacaa
     1261 gcggtaaatg atataggtga attgtggaaa aaatttatgg gcaaagggat atataatgct
     1321 ataaagggta aaaagaatga caagacaata ggtctatata cagattacca aggtgatttt
     1381 acaagtccgt atagctttgt tgcatgttgt gaagtaaata gtaattctga taaagagaag
     1441 aatttagaag aattaaacac taataatact gttggagaaa gtattattag taaagttata
     1501 ccagcagata agtatgcaaa gtttgttata gtaggaggtc aaaaagaagt aggagatttt
     1561 tggtttaagt tttggcaaat ggattttgat aggacttata taagtgattt tgaagagtat
     1621 caatgtaata cttttgatac aaagaaacaa gaagttcata tttacatagg aataaagtag
     1681 atttgttatt actatattga atgaagtatt gagtatagta catttattat agatagtttt
     1741 attatttata actataagca gtaaatctgt aaaagattta ctgctttttt agtaattaat
     1801 agtatcagta tcactttgaa tcttcagaca tattcttcat ttaatattgt ttattgataa
     1861 atgacacttg taaatataat gatttgttat ttaatttaaa atatgaaaaa aagtattata
     1921 aacacattag aatacttctg gatttatggg tatacaattt gaaaaatata aaa
//
LOCUS       DACXMZ010000362         1968 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.363, whole genome shotgun sequence.
ACCESSION   DACXMZ010000362 DACXMZ010000000
VERSION     DACXMZ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.363"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            62..460
                     /locus_tag="JD016_19010"
     CDS             62..460
                     /locus_tag="JD016_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001710.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    896..1159
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1310..1861
                     /locus_tag="JD016_19015"
     CDS             1310..1861
                     /locus_tag="JD016_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HAU5001711.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          827 a          186 c          296 g          659 t
ORIGIN      
        1 taatttttag ttatatatat attataatga actagttaaa aatttttatg agggggaaaa
       61 tatgaataag aagaaaaaat tgattgggat agctatatct tttctactta taatcagcat
      121 tataaatcca ttacaagtag aagcagaagc tattattgag tctagtgtat acatttcttc
      181 aataaaagag aattatacat atgataatac taaatttaaa actattaaat ctaaaagtag
      241 acataaaagt aggaaatata ataaaagtaa agtaaagttg aaaaaacgaa gtataagaac
      301 taaaaagtat ggatatcaaa ctataaataa atataaaaga caaaacgcaa gaagacataa
      361 tatcctaaca aaattaatat tgtccataat agtcatattt ttggttatag ttttagtagt
      421 gctaatattt attaaaagaa agaagaaaaa attttactaa aaataattaa aagaatatca
      481 tttttttaat cattggtatc tatttaataa tatgatgtaa ttaaaatgga gtaatattct
      541 gtttcaaagg aggcatataa atacaggaat gagtattaaa tgatggttaa ttaagtaaat
      601 ttattgataa atgtacttaa ttaactttga agaatttaat tcctcatgaa tcaaaatata
      661 aaattacatc aacagttatt cacaaaatta atataagaag aattagagcc aaagagcaga
      721 ctattagacc attaaagtaa ttattcatag aattttgtat atgaaaagaa atgtatagta
      781 taatttactt tttttataaa aaatagatta caaaatatat tttttataaa aaggtattga
      841 cattttttag aaaaaagaat atcattattt taacaacaaa attcaataca ataaattaaa
      901 tcctatgaca aggaagagta gttaaattta ggttcagtat agagagctga ggatggtgga
      961 atcttagcaa gaaaaaattt gatgaatggc ccttagagga ataaattgaa atcatagttt
     1021 tgagagtaga ctttatcgtc agtcgcacgt tatagcgaat aaggtatgta agtacctgat
     1081 aatgagatgt atgaaaattg aattttatat taatttttat ataatttagg tggcaacgcg
     1141 gataatctcc gtcctattag tttaggacgg agttttttat tttactgaaa atttaaaaga
     1201 tagaaggtgg tgagaaagat gttatgaatt tcaaaagcta aagatgatga tatagtaggt
     1261 tggcaaaaaa ataaattgaa aattaaaaaa ataggaggaa gtacaaaaaa tgaaaacaaa
     1321 tacaaaaaaa ttaactttaa atgcaatact tttagcgatg ggattattaa tacatcaaat
     1381 cacaccagct ataggattac caatgaagcc agatgtacca ctagcaatgt tatttgtaat
     1441 attggtttta aatagagatg attataaaac ttgtttgata gcgggtatag taacaggaat
     1501 atttacagca cttacatcaa gttttccggg aggtcaaatt cctaatgtaa tagataaaac
     1561 tcttacagca aatatagtat ttttattaat gaatattagt tataatatgc cttttataaa
     1621 aaaattagct aagaaaacac aagatactgt agtagttgca ataataatgc caataggaac
     1681 tcttgtaagt ggaactatct ttttattagc agctcaagct atagttggtc ttccaggggc
     1741 ttcatttaca gctttattct tagcagtagt attaccagct gttttaataa atctagttgc
     1801 aggaatattt ttattcaaga ttgttagtct atcaataaga agagttagtt atcaagctta
     1861 aacacgaaat aatattttac tttataaagc attataataa ctaaataaca taagaataaa
     1921 gtagattata actattttat tcttatgttg tttaaagatt tggtatta
//
LOCUS       DACXMZ010000363         1964 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.364, whole genome shotgun sequence.
ACCESSION   DACXMZ010000363 DACXMZ010000000
VERSION     DACXMZ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.364"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            160..735
                     /locus_tag="JD016_19020"
     CDS             160..735
                     /locus_tag="JD016_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001712.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            833..1831
                     /locus_tag="JD016_19025"
     CDS             833..1831
                     /locus_tag="JD016_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HAU5001713.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
BASE COUNT          811 a          171 c          335 g          647 t
ORIGIN      
        1 gctttattct atatgattct aagatagagg atttataagt gtttaaggtg aattaaatat
       61 aagacaactt cttaaagcaa atgggtgtgg tatttaaatc taaatgtaga catataatat
      121 taaatatgct aatatatcaa aataataagg ggggaattta tgaagcttaa agaagcattg
      181 attacatgtg ttgtatgtag tgtatgttta actccattat tggtaaatgg agaaacaaaa
      241 atagatgcag atcattgtaa agaatctata gtaaatatag atactatagt aaagtcaggt
      301 aaaagcttta attccaagta taatcaaggt atggaagatt ataaaaatag tgatgagtat
      361 aagttgaaag tacaaaagga gaaactacaa aatcagttag gtattaaaat aaagaagtta
      421 gtacctataa cttttgaagt tagttattac acaagtctta atcaagaaaa tggatatgga
      481 gcgataactg ctacaggaga tgatttagaa gatggttttg tagcaaacaa tcacttaagt
      541 tttggaacta agattattgt agatggaaaa ttaaaaactg ttatggatag ggggtctagt
      601 aagtatttta gtaattctaa tgcaatagat gtatttgtgc ccaaatggag taatgaaagt
      661 gaatcagagt actatgatag agtcaataat atgggaagac attacaaaaa aggctacatt
      721 attgttgaag aatgatgttc tttgaagtaa tatgtattga ttgtagtatt taatatatat
      781 ataataaata taaaactatt agtacaatga gatatattac taggagggca atatggaaca
      841 atcgtttata aatggaaaag taaacttatt aatagaaaat tacaaagcat taaatgaagt
      901 taaaggttct tggcaaatgg gattgataca acatagttgt gcacttgcat ttacccttaa
      961 gaacaagaga ataagtccta gattagtgga agaacgaata gagcttatta aaaaaaatac
     1021 tggattattc tctaatttta gaggatataa tatgttttat atggcaacac tactttcgtt
     1081 tgaatcaaat ccagaaagta gttttaagat gattttagat atatacaaag aacttaaaag
     1141 tgaaaagttt tggggcgata catatttacc acttacagca tcaatagttt atgaaaatag
     1201 agagaaaatg gattatctta catgtatatc aaaaatgaaa ataatatatg attacatgag
     1261 gaaaaaacat ccatttctaa cttctagtga tgactattgt aatatagcac ttattgcaat
     1321 tcattcaaaa aatttagatg aagatttaga atacatagaa aaatgttatg aatttctaaa
     1381 tgaaaatggt ttttataaag gaaatgattt acaagcatta tcccaaattc ttttatttag
     1441 tgatgataga actatgctta aatgtaaaaa gactattgaa cttaaaaaag catttaaaga
     1501 aaatgattgt aaaatgaatt attatggata tccaataata ggagcaattt cattattaga
     1561 ttatagagaa gatgaaataa tagaagagat aaaaaatgtt tcaaataaac ttaaagaaga
     1621 aaaaggcttt ggaaattggt ctttaggaaa aagtaataga gtaatgatta gttctgctat
     1681 tgtagcttca atttatgcag atttgatacg aaatgagaat aatataggct ctataactaa
     1741 taatatattt ttaaatattg ttgttgctat tcaaattgca tgtgtaatgg cagcaacaag
     1801 tgcagctata gcatcttctt catcaaatta ataagattta ttgaagttgg atatattaaa
     1861 tttaataata caatttatat gtttaaaaag aagatagtat tacattttaa ttaactgtga
     1921 tattatataa tatgaaacat ataattaata tatagatttt aggc
//
LOCUS       DACXMZ010000364         1942 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.365, whole genome shotgun sequence.
ACCESSION   DACXMZ010000364 DACXMZ010000000
VERSION     DACXMZ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.365"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            53..427
                     /locus_tag="JD016_19030"
     CDS             53..427
                     /locus_tag="JD016_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAU5001714.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            424..1287
                     /locus_tag="JD016_19035"
     CDS             424..1287
                     /locus_tag="JD016_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001715.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1287..1925
                     /locus_tag="JD016_19040"
     CDS             1287..1925
                     /locus_tag="JD016_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAU5001716.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT          743 a          204 c          298 g          697 t
ORIGIN      
        1 tatatttata taatgtgtat atactatata tacaatttaa ggaggtgtct tattgaatat
       61 agttattagt aattcgagtg ggaagccaat atatgaacag attactacac aaattaaaag
      121 tatgattata agtggagagt taccagaagg ctcagctctt ccaagtatga gactattggc
      181 aaaggaactt agaataagtg taattactac aaaacgagca tatagcgatt tagaacgaga
      241 cggatttatt gagacagtta ctggtaaagg cagttttgta tctagtaaaa acatggattt
      301 tgtcaaagaa gaacaatttc gtttaattga agaataccta caaaaagcag ttgatacagc
      361 aaaaagtagt gatattacat atgacgaatt aaaagatatt ttatttttat tatataaagg
      421 agaatgataa gtatggatgc aataactata aaaaatttaa ataaaactta taaagatttt
      481 agcctacaag atatatcttt ttcagtacca aaagggagtg taatgggatt tgtaggtgaa
      541 aatggtgcag gaaaaacaac tacattaaaa gcaattttaa atttaatatc ttatgatagt
      601 ggaaatatag aaatctttgg tcttgatagt aaaaaaaatg aaaaagaaat taaagagcaa
      661 attggggttg tatttgaggg aagcaacttt catgaaaatt taaacactga ccatgtttca
      721 aaaatcatga gtaaaatcta taaaaattgg aatgacacct tatttaaaga ctacttaaaa
      781 aaattgagag taccagacaa taaattaatt aaggagtttt caaaggggaa taagatgaaa
      841 cttagtattg ctgtagcctt atctcataaa ccaaaattat taatactaga tgaggcaact
      901 agttcacttg accctatcgt tcgtgaagaa atactagata tatttttaga ttttatacaa
      961 gatgaagaac actctattat cttatcttca catattacaa gtgatttaga taagatagca
     1021 gactatatta catttataca taaaggaaaa attgtattta gtgaaaataa ggatgaatta
     1081 attgatacta tgggagtttt aaaatgtaaa cctagtgatt ttgataacct ttcaagagag
     1141 gattattcat attatcgtaa aagccaattt ggatatgaag tacttcttaa agataaacat
     1201 agatttatct caagacatcc aaactgtatt gttgataata cgtctataga agaaattatg
     1261 ttattttatg tgagaggtga taaataatga aaggtctaat tttaaaagat ttacttaatt
     1321 tgaaaggaaa tataaagttt atacttttat tcatcattat gtttggcttt atgtcttctt
     1381 taggagatgg aaatgttaat aactttatag gtgttattat agttttatgt actacaatga
     1441 ttgtttcaac attttcatat gatgatttga ataaatggga tagttatgta cttacaatgc
     1501 ctattaatcg caatgatatt gtattaagta aatatttaac tatgttaata tttagcttta
     1561 taggtgttct tgtttcactt attgttagtg taactattgg ttattttaaa aatacactta
     1621 tattaaatga aacactactt attaatgcac taatcttatc aatttcagta tgttttggta
     1681 gccttattct accacttatt tataaatttg gtacagaaag agctcgttta ttaatgattt
     1741 tatgcttttt agttccaaca ctagctttat tagtttttaa aagtatttta gaaaatatta
     1801 gttcacctat ttctatagaa attatactta atacactagt atattctctt ccatttgttg
     1861 ctattttact atttgtgatt tcatatttta tatcatcaaa aatctatagt aaaaaagaag
     1921 tataaaatat actatatata tt
//
LOCUS       DACXMZ010000365         1887 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.366, whole genome shotgun sequence.
ACCESSION   DACXMZ010000365 DACXMZ010000000
VERSION     DACXMZ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.366"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            128..244
                     /gene="rrf"
                     /locus_tag="JD016_19045"
     rRNA            128..244
                     /gene="rrf"
                     /locus_tag="JD016_19045"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            251..325
                     /locus_tag="JD016_19050"
     tRNA            251..325
                     /locus_tag="JD016_19050"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:283..285,aa:Asn,seq:gtt)
     gene            330..404
                     /locus_tag="JD016_19055"
     tRNA            330..404
                     /locus_tag="JD016_19055"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:363..365,aa:Glu,seq:ttc)
     gene            410..485
                     /locus_tag="JD016_19060"
     tRNA            410..485
                     /locus_tag="JD016_19060"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:443..445,aa:Val,seq:tac)
     gene            491..567
                     /locus_tag="JD016_19065"
     tRNA            491..567
                     /locus_tag="JD016_19065"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:525..527,aa:Asp,seq:gtc)
     gene            577..651
                     /locus_tag="JD016_19070"
     tRNA            577..651
                     /locus_tag="JD016_19070"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:609..611,aa:Thr,seq:tgt)
     gene            666..750
                     /locus_tag="JD016_19075"
     tRNA            666..750
                     /locus_tag="JD016_19075"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:700..702,aa:Tyr,seq:gta)
     gene            760..843
                     /locus_tag="JD016_19080"
     tRNA            760..843
                     /locus_tag="JD016_19080"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:794..796,aa:Leu,seq:tag)
     gene            873..949
                     /locus_tag="JD016_19085"
     tRNA            873..949
                     /locus_tag="JD016_19085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:907..909,aa:Arg,seq:tct)
     gene            957..1032
                     /locus_tag="JD016_19090"
     tRNA            957..1032
                     /locus_tag="JD016_19090"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:990..992,aa:Gln,seq:ttg)
     gene            1121..1209
                     /locus_tag="JD016_19095"
     tRNA            1121..1209
                     /locus_tag="JD016_19095"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1155..1157,aa:Ser,seq:tga)
     gene            1213..1288
                     /locus_tag="JD016_19100"
     tRNA            1213..1288
                     /locus_tag="JD016_19100"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1246..1248,aa:Phe,seq:gaa)
     gene            1295..1368
                     /locus_tag="JD016_19105"
     tRNA            1295..1368
                     /locus_tag="JD016_19105"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1329..1331,aa:Met,seq:cat)
     gene            1383..1459
                     /locus_tag="JD016_19110"
     tRNA            1383..1459
                     /locus_tag="JD016_19110"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1417..1419,aa:Ile,seq:cat)
     gene            1483..1559
                     /locus_tag="JD016_19115"
     tRNA            1483..1559
                     /locus_tag="JD016_19115"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1517..1519,aa:Pro,seq:tgg)
     gene            1567..1643
                     /locus_tag="JD016_19120"
     tRNA            1567..1643
                     /locus_tag="JD016_19120"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1601..1603,aa:His,seq:gtg)
     gene            1652..1727
                     /locus_tag="JD016_19125"
     tRNA            1652..1727
                     /locus_tag="JD016_19125"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1685..1687,aa:Lys,seq:ttt)
     gene            1735..1808
                     /locus_tag="JD016_19130"
     tRNA            1735..1808
                     /locus_tag="JD016_19130"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1767..1769,aa:Cys,seq:gca)
     gene            1815..1886
                     /locus_tag="JD016_19135"
     tRNA            1815..1886
                     /locus_tag="JD016_19135"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1847..1849,aa:Asn,seq:gtt)
BASE COUNT          474 a          421 c          498 g          494 t
ORIGIN      
        1 tttgatgttc attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaat
       61 aaaaaaagta ttgacaaaaa tagtaaaaaa tgttaatatt atacttgtcc tagcaacaag
      121 aaaaaaatgt ggttataata gcggagaaga tacacctgtt tccattccga acacagaagt
      181 taagttctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      241 acgtgaatgt tccaaggtag ctcaacggtg gagcaaccgg ctgttaaccg gtaggctgtg
      301 ggttcgagcc ccacccttgg agccattatg gctcgttggt caagaggtta agacaccgcc
      361 ctttcacggc ggtaacaggg gttcaattcc cctacgagtc accatttgtg ggatcatagc
      421 tcagctggga gagcacctgc cttacaagca gggggtcaca ggttcgagcc ctgttggtcc
      481 caccatttgc ggcctggtag ttcagttggt tagaatgcca gcctgtcacg ctggaggtcg
      541 agggttcgaa ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc
      601 agctgacttg taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt
      661 aatacggagg atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact
      721 tcggtggttc gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca
      781 gacgcactag acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca
      841 ccatttttgt ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa
      901 cgcccttcta aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg
      961 gattagccaa gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg
     1021 catccccagc caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata
     1081 gttaggttca attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt
     1141 taaggagctg gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc
     1201 tctccgccaa atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc
     1261 ggtggttcga ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc
     1321 gttcggttca tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat
     1381 gtgggccatt agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg
     1441 agtccctgat ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt
     1501 ggtagcgcac atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa
     1561 tataaggtgg gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag
     1621 ttcgactctc attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct
     1681 gacttttaat cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac
     1741 atagccaagt ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt
     1801 cgcctccaaa atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc
     1861 tgtgggttcg agccccaccc ttggagc
//
LOCUS       DACXMZ010000366         1877 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.367, whole genome shotgun sequence.
ACCESSION   DACXMZ010000366 DACXMZ010000000
VERSION     DACXMZ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.367"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            101..715
                     /locus_tag="JD016_19140"
     CDS             101..715
                     /locus_tag="JD016_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAU5001717.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDIAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            733..1095
                     /locus_tag="JD016_19145"
     CDS             733..1095
                     /locus_tag="JD016_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5001718.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1201..1698
                     /locus_tag="JD016_19150"
     CDS             1201..1698
                     /locus_tag="JD016_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HAU5001719.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          711 a          212 c          322 g          632 t
ORIGIN      
        1 aacgtttttt gacaagtctt tattttgtct aaattaaaca aactttttta caaataaata
       61 cataaaattt aaactaatct atttattgga ggagataaaa ttggaagaag taattgcaaa
      121 aattattact atagaaaatg gattcaaaga aattgaaaac attgctaaaa aaattgtaaa
      181 aaataatgac tcaaaaaagt gttattatct atcaatctac ttatataggt cagaatatta
      241 tcaagtaaga gaattggctg tgtttatact tggttttata tcagtaacta tttctgaagc
      301 tttgttattt ttaaaagata atgtgagtaa agatgaaaac tggagagtac aagaaatact
      361 agctaaatca tttgattatt attgcagtga agtaggatat gaaaaggcac ttcctgttat
      421 aaaagaatgg cttaattctg atagtccaaa cataaaaagg gctgtgacag aaggtcttcg
      481 aatttggact agtagagact attttaaatc taatcctgac attgctatat cactactttc
      541 tagtttaaaa gaagatgata gcgaatactt gagaaagtct gtaggtaatg ctcttagaga
      601 tataagtaaa aaatatccag atttagtaaa aaaagaatta gattcatggg atatatcaaa
      661 taaaaaagtt gagtttgtat ataagcttgc ttcaaaatgt atcttggcaa agtaaaatgg
      721 aggtgaactt atttgaaact tcgtgatgag tatacttgcc cattagaact aacacatgat
      781 atgataaaag gcaaatggaa atctattata ttatggagat tacgcatagg accaacttcc
      841 ttatcaaaac ttgaaaagga aatcaatggt attaatcaaa agatgttact tgaacaactt
      901 aaagaattaa ttgaatttgg tcttgtagat aaaaagacat atgaaggtta cccacttaaa
      961 gtcgaatact cacttacacc tattcgaggg aaacaaatat tggatgcttt ggctataatg
     1021 caagatatag gaattagctt tatgttggat aatggtatgg aagatattct taaggaaaaa
     1081 ggtgttttaa agtaaataga tacccacaaa aaagtaagta atttaccatt attttcaact
     1141 ggtatataat taaattatag atatattcta ttaatttatt ataataggag gtaaattatt
     1201 atgaaagtaa ctagtacagg tatagtaaat ggtgttatag aagataaata cggtaaaaga
     1261 ggaactcagt tcaatgaagg tggtatgcct acatattcac tacctctaaa gtttgaagat
     1321 gctccaaaga atacagtatc ctttgctgta tttttagaag ataaagactc tgttcctgta
     1381 tgtgggtttg catggataca ttggttagct gtaaatataa caaaagatga gttaaatgaa
     1441 aatgaaagtg taacagctac tgattttgta caagggacta ctagttggca tggcaaacta
     1501 ggaggtcttg acagattaga ggccagtgta tatggtggaa tggctcctcc agacagacct
     1561 catgtctatg aaatacatgt atttgcactt gatactattc ttgaccttga aaaaggattc
     1621 tatatgaatg aatttttcaa agctattgaa gggcatgttt tagatacatt tacactaaaa
     1681 ggaagttatt ctaattaatt aggttgacta tagctaatca ttaattaaat gctacattta
     1741 attgatgatt agcttaattg ttattagaat attttctgca ctttatctta atatatattt
     1801 tagttgactg gtattttaat ttctattaga ttattatcaa tttatataaa ttgattttag
     1861 cttaaaactt acttatc
//
LOCUS       DACXMZ010000367         1830 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.368, whole genome shotgun sequence.
ACCESSION   DACXMZ010000367 DACXMZ010000000
VERSION     DACXMZ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.368"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            96..443
                     /locus_tag="JD016_19155"
     CDS             96..443
                     /locus_tag="JD016_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAU5001720.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(593..1642)
                     /locus_tag="JD016_19160"
     CDS             complement(593..1642)
                     /locus_tag="JD016_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAU5001721.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
BASE COUNT          618 a          278 c          250 g          684 t
ORIGIN      
        1 agaattttat tcctattgtg taaagtgaga atactatttt cttatattgg tataataata
       61 gtaaatctat aatatagaaa agaggtattt ctagtatgca tgaaaataaa aagaatctat
      121 tagacggaag tttagctcaa tataatgata cagcagttcc aaaaatacca gtatttgctg
      181 gtaatgatat aacttcagaa gtttattatt ttaaacctaa tcaagttctt aatgctcata
      241 gacaccctaa tggtgaacaa atatttgtgt tcctaaaagg agaaggtaaa atgaaacttg
      301 gtgagcatga atgtgatgta aaaaacggcg atactgtatt tgtaccaaca ggagagtggc
      361 atgaaattac taatggaagc aatgaagaaa tggtagccgt tcaaattaca aaaattaatg
      421 ctggagcaga atacagagga tagatatata agtacagtta gtactttaat atctatttta
      481 ttatataaac tttaatctca aatataaaaa gagtctttta aaaggctctt tttatatttt
      541 tgttaatttt cattataaaa aaaagaaaat atctagctaa aactaatata ttttatttaa
      601 tattttttat agcattgttt gcaaactcag tatttacgat atctttataa ggtgcttcct
      661 tatctagttc tttagcttct ttcataacag tcatcaattt cttcaaacct tcctctttta
      721 atacaggatt ttgagaccat gcatctattg atttatatct atctacaact gttgttaaat
      781 cttctaaaga tatatcttca aagaaaggtt gcatagcctt agctacttct ttgctagatg
      841 cttgttgaat ccataattga cctttataaa cagcgtttgt aaatctttga attaaatctt
      901 tattgttttt catgtatgat ctcgtagtag agtatgctgt aaaaggtatt tctccagaat
      961 cttttcctat agatgtaact atatatccac taccttcttt ttccattgct gttgctgtag
     1021 gttcaaatgc tgttgtaaag tcaccctctc cacctgcaaa agctccagcc attacaccaa
     1081 attgtacatc agttcttaca ttaacattgc ctgcttttag attttctcca atagtaagac
     1141 ctttttcttt tagaacatat tcaaaagtca ttagtggaac tccacctttt ctaccaccta
     1201 atatttctgt tccttttaaa tcattgtaag agaaattagg catttttttc ctagcaacta
     1261 gaaaactacc atctcttttt gtcaattgag caaagttcac tgcataatcg tttttgtttt
     1321 gattatatac atatatagat gcttcaggac ccattaaacc tacgtttgct tcacctgaaa
     1381 tcaaagctgc catagtttta tctgcacctt gggtatttat aaggtcaatc tttatacctt
     1441 catcttcaaa gaatcctttc gtaatagcag catactgtgg agcataaaaa acagagtgag
     1501 taacttctgc tatagttact ttatccagat ttttagacga agaagattca tcttttttgt
     1561 cattagagtt aaaatcacat ccagttagac atattgtact tacaatgata gagcttgcta
     1621 ttgcgactat ttttttaaac ataataaata cctcccataa gacatacgaa tagtatatag
     1681 tattaaaaat ttattgtagt tgttacaaaa atgtatctat gcttataata tttctacaaa
     1741 aacttaataa taattttcag ttttatgttt agttttttta gtaatgtgta atattgaata
     1801 aattatattg attatttata aactaagtaa
//
LOCUS       DACXMZ010000368         1815 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.369, whole genome shotgun sequence.
ACCESSION   DACXMZ010000368 DACXMZ010000000
VERSION     DACXMZ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.369"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            106..1704
                     /locus_tag="JD016_19165"
     CDS             106..1704
                     /locus_tag="JD016_19165"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HAU5001722.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
BASE COUNT          701 a          219 c          274 g          621 t
ORIGIN      
        1 attttttcac taaatttttt tctttttaca acaaatactc actacctttt gatataatat
       61 ttttatcaat tattattata ttcaagtttt gaaaggagta ttaaaatgag tttattagtt
      121 ttcggacata aaaatccaga tacagattca atatgttctg caatttcatt agcacattta
      181 aagaacaaac taggaataaa agcaacagct tatgctttag gagatatcag aaaagaagct
      241 aaatatgcct tggattactt taaggtagat gcacctgaaa ttttagataa tgttagaata
      301 caagttagag atttaaatta tgataaagta gtacctttaa caccaacttc atctatatta
      361 gaggcttata accttatgga tgaaaaaaat gttaaaacac tacctgtaac ttatgatgat
      421 ggaacttttg ctggtatcat tactatgaaa gaaatagcta aaaatctact tcatcaacac
      481 tttacaacta tacacacatc actatcaaac atttgtaaaa accttaatgg tactattctt
      541 gtggactgtg gtgaagatat aaaaggtaga gtagtcactt tatcatttgg aatggagact
      601 ctaatagaaa ctttaaaaga aggagacata gctattgttg gagatagata tgatagtatt
      661 gaatatgcta ttgatacaaa agttaaactt ttaatactta ctaatcatac agagatttca
      721 aaagatttgc tagctcttgc taagataaat ggagtgtctg ttgtttctgt tgaaagtgac
      781 aattataaaa cttctaatgt aataaatcaa tgtagtttct tagactctat agaagctaca
      841 gaaaatttaa ttacattcaa agaagatgat tatatggatg aaataaaaga gattatgcta
      901 gaaacaaact tcagatctta tccaatatta gatgatgata ataagttttt aggtttagtt
      961 tcaaaaggtc atctattaaa cccatctaaa aagaatgttg ttttagttga ccataacgaa
     1021 tatgctcaaa gtgctgatgg aattgaacaa gcaaacatag ttgaaataat tgaccatcat
     1081 aaacttggtg gtatttctac tgatgttcct atgtcattta gagttatgcc tgttggatgc
     1141 aatagtacta taatttatca aatgtacaaa gaaaataatg ttgagatacc ttatgaaata
     1201 gctgggcttc ttttatctgc tatattatca gatacattat tatttaagtc tccaacaaca
     1261 actgatatag ataagaaagc ttgtgaagaa ttaagtaaaa tagcaaaagt agacatggaa
     1321 aaatacgcta tggatatgtt taaatgcggt acttctttag atgaatatac tatagaagaa
     1381 atagtaaata tggactttaa agaatttaat atgagtggac atagagttgg tattggacaa
     1441 gtatttacct tagacataga ctctatattt gctaaaaaag atgagttttt atcttatata
     1501 aattcgactg attatgataa attaattctt tcagttacag atataatcaa agaaggttct
     1561 tatttaatct ataaagcaga agatagctta attgctaacg catttggtgt tgaaggtgtt
     1621 caaggtacat ttgcaccagg tgttgtatca agaaaaaaac aattagtccc taatttaact
     1681 actgcaatca aaaattttaa ataatataaa atttttaaat aacgaaaact gtctcaaatc
     1741 tatatttgag acagtttttt attatatata tatattgata ataaaggtaa ataaaaatcc
     1801 ataaatttaa aattt
//
LOCUS       DACXMZ010000369         1814 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.370, whole genome shotgun sequence.
ACCESSION   DACXMZ010000369 DACXMZ010000000
VERSION     DACXMZ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.370"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            74..652
                     /gene="mocA"
                     /locus_tag="JD016_19170"
     CDS             74..652
                     /gene="mocA"
                     /locus_tag="JD016_19170"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HAU5001723.1"
                     /translation="MIYMINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKID
                     FHQVVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQ
                     PFIDNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTIL
                     KNSSKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            649..1668
                     /locus_tag="JD016_19175"
     CDS             649..1668
                     /locus_tag="JD016_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HAU5001724.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT          728 a          164 c          347 g          575 t
ORIGIN      
        1 ttatatatat agataatatc aatagagata ttataaaaat catagaacta tcaatatatt
       61 ataaatacca aggatgatat atatgataaa tgcagtgata atggcatcag gctttgccag
      121 aagaatggga gcaaataaac ttttattaaa ttataaaggc tgttctataa tagaacatgt
      181 atttagagaa gttagcaaga ttgattttca tcaagttgta gtagtaagtc aatatgaaga
      241 ggttttgtta ttgtgtaata aatatggttt tgaatatata gataataaaa atgcaaatat
      301 aggtcaaagt gaatctataa aattaggagt tttaaatagc tcaaaatgtg atggttatat
      361 gttctttgta ggagaccagc catttattga taacttatat atagataaaa ttataaatac
      421 ttttaaatta gatagagatt ttatagtaat tccaagatac aaagatatgt gtggaaatcc
      481 agtcatattc ccatatagta agaaagaaga attgttggca cttaaagaag atgaaaaagg
      541 taaaaccatt ttaaaaaatt catcaaaaat aaagtatgta gaagttccta aagaaatgtt
      601 atttgatatc gatacaaaaa tggactatga gagaattggg ggtaaattat gaagctaata
      661 aaaactatag atgctgttgg acaagtttta tgtcacgaca taactcaaat aatacctgga
      721 gaatttaaag gaagaaaatt taaaaagggc cacatagtaa aagaagaaga tattccagtg
      781 ttattatctc ttggtaaaga taatttgtat gtatgggaaa agtcagaagg aatggttcat
      841 gaaaatgaag gagcattgtt tttaaaagaa ctaactgctg gagaaaattt agattttagt
      901 gaaataaaag aagggaaaat agattttata gcagcatgtg atggtctatt aaagatagat
      961 gttgatgcac tttttgattt gaattgtgtt ggagaaataa tgatggctac tcttcataat
     1021 aattttatag taaataaagg tttaaaagta gctggaacaa gagtgatacc tttagttata
     1081 gatgagaaaa aattagaaga tgcaaaaaaa gtagttggta atagaaaaat agtaaatgta
     1141 gtacctttta aacctaagaa agtaggaatt gttactacag gaaatgaagt gttttatagc
     1201 agaattgttg ataaatttgg accagtaata gaagaaaaag taaaaggctt tggttgtgaa
     1261 gttataggac agactatatg cccagatgat aaagagatta taaagagtgc aataaaagaa
     1321 tttataagtc aaggagcaga acttatctgt tgtactggtg ggatgagtgt agacccagat
     1381 gatgttacgc caacagctat aaaagaaact ggagctgatt tagtgacata tggttcacca
     1441 atattaccag gagctatgtt tttacttgct tattacggag aagttccaat tatgggtata
     1501 ccaggatgcg caatgtatca caaaactaca gtatttgata tagttttatc tagggtttta
     1561 attgatgaaa agcttgataa gtatgatata gctagatatg gtcatggtgg cctttgtatg
     1621 aactgtgatg tatgtactta tcctgcatgt aattttgcta aaatataaag ttattaaaaa
     1681 agagtattta caaattaagt actcttttta aatatgaaac aaaacagcta gaatgatgtt
     1741 ttaaaaaaag catatttatt tagaaattat aataacttat tttcaatgtg taaaagaatg
     1801 aataaagttg caat
//
LOCUS       DACXMZ010000370         1778 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.371, whole genome shotgun sequence.
ACCESSION   DACXMZ010000370 DACXMZ010000000
VERSION     DACXMZ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.371"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            118..747
                     /locus_tag="JD016_19180"
     CDS             118..747
                     /locus_tag="JD016_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAU5001725.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYKTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            722..1126
                     /locus_tag="JD016_19185"
     CDS             722..1126
                     /locus_tag="JD016_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAU5001726.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            1129..1605
                     /locus_tag="JD016_19190"
     CDS             1129..1605
                     /locus_tag="JD016_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAU5001727.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEVRLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
BASE COUNT          749 a          197 c          236 g          596 t
ORIGIN      
        1 attatccata aaaatatatt aacttatatt atttaataat ttattgacag tattaaaatt
       61 aaattataca ataacagtgt aaattaactt aacaccgtta agcaaagagg tgattatatg
      121 agtatcaaag aaagacgtaa aaatgaaaaa gaagaaatga agaaaaagat tatggatgct
      181 agcatcgaaa ttataaacca acatggatat gaaaatttat caataagaaa aattgctaca
      241 aaaatagaat attctccaac tacaatttat ctttactaca aagacaaagc agagattatc
      301 agcgatatga ccaataaact ttataaaaca gtagaaagta atgctattga cattatgaat
      361 aattgctcat cactaccaat agataaacaa atcatagaaa ttatgactag ctttataaaa
      421 actctttcta gtgaaccaga gatggcaaaa gctatcatgc atagtagaat gaatattata
      481 tttgcatctg aaaatacaaa caatacgcct agcaataatg gtataaaaat gcttgataaa
      541 ttcctttcca ttggaattga acaaggaata ttcaaaaaaa atatagataa aagctcatgg
      601 atgcttataa gtgcattgtt aggattcgtt ttatgtgttg ttgaaaataa actatattct
      661 ttacataatt tttctcaact tatagaaaat ttcttagata ttttagtggg aggactttac
      721 aatgaaaatt cgcaacaagg ctactaataa aaagaaaaaa tatgtaaaaa tatctattgc
      781 tctattcttt gtatttatat gcattattat tgtaattgta agcaacttaa agccagaaaa
      841 cttggtagta aaagatatag atattaataa tgttaaaaat ggcatttata ctggaagtgc
      901 tgataataac ttggtaaaag ctactgtgtc tgttgaagtt aataatggta aaatccaaaa
      961 tattaatatc ttaaaacacg accatctttt aggtaaacct gctgaaaaaa ttacaacaag
     1021 tattattaaa caacagtctt tagatgtgga tgctataact tctgcaactt atagcagtaa
     1081 tactatacga aaagctgtag aaaatgcttt aagaaaggga gaataattat gaatactatt
     1141 attatatatt caagtaaata tggttgtaca aaagactgtg caaatatact aaaaaataaa
     1201 ctatcagata atgtcactat tgttgatatt aataataata ataataaaat tgagttatca
     1261 aaatttgata aaattattat tggaagttct atatatgttg gttcagtttc aaaaaaaata
     1321 caagtgctat gtaatgataa tgtagagtta ttaaacaaaa aacaagttgg cattttcctt
     1381 tgctgtggct tttctgaaca agcagataaa tatttaaaat caaattttcc atcctcctta
     1441 ttagaaagtg caaatgctat tggaattttt ggtagtgaag ttcgattaga aaaaatgaaa
     1501 tttttagata aacttattat gaaggctgtt tcaaaaggta attatgacag ttttagaata
     1561 tcacaggata atatagataa ttttttaata aatcttaata gctaaaataa aactaattat
     1621 ttctatgata aattctttta tttataagta atattgctca aaatagtctt taaactttat
     1681 caaaatttta tttttcttag tgaaatctaa tgaaactagc acttaatata tactttatta
     1741 atcataacaa atatgtttta ttaattataa caaaaaga
//
LOCUS       DACXMZ010000371         1778 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.372, whole genome shotgun sequence.
ACCESSION   DACXMZ010000371 DACXMZ010000000
VERSION     DACXMZ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.372"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            127..1419
                     /locus_tag="JD016_19195"
     CDS             127..1419
                     /locus_tag="JD016_19195"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAU5001728.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT          689 a          188 c          356 g          545 t
ORIGIN      
        1 taaatttgta cctaattttc aaaagcaact ttatgtacta taatatagaa agtagattaa
       61 aatagtagac ggatgtttac agtaaatgaa aatttaacta atagaaaatc gaagggagat
      121 atacaaatgt cagttattga attagtatat gctagagaag tattagactc aagaggaaat
      181 ccaactgtag aagttgaagt agtattagaa gatggagcaa tgggaagagc aatagttcca
      241 tcaggagcat ctacaggagc ttttgaagct gttgaattaa gagatggaga taagggaaga
      301 tacttaggaa aaggtgtaga aacagctgtt gctaacgtaa atgaaataat agcacctgaa
      361 atagaaggaa tggatgcatt tgaccaacca gctatagatg caatcatgat agaattagat
      421 ggaactccaa acaaaggtaa attaggagct aatgctatat taggtgtttc aatggcagta
      481 gctagagcag ctgctgatga aataggttta ccattattcc aatacttagg tggagtaaat
      541 gctaaacaat tacctgttcc aatgatgaac atattaaacg gtggagagca tgctgataac
      601 aacgttgatg ttcaagaatt tatgatatta ccagtaggag cttgttgttt caaagaaggt
      661 ttaagaatgg gagcagaagt attccattca ttaaagaaag ttttaggaga aaaaggatta
      721 gcttgtggtg taggtgacga aggtggattt gctccaaact taggttcaaa cagagaagct
      781 ttagaattaa tagttgaagc tataacaaaa gctggatata agccaggtga agacgttatg
      841 ttaggtcttg acgttgctgc tacagaaatg tacaacaaag aaactaaaaa atacgtttta
      901 gcaggagaag gaaaagagtt aactgctgct gaaatggttg ctttatatga agattggtca
      961 aacaacttcc caataataac tatagaagat ggtttagacg aagaagattg ggatggatgg
     1021 aaattattaa ctgaaaaatt aggaaataaa ttacaattag ttggtgacga cttatttgtt
     1081 actaacacag agagattaga aaaaggaatt gaaaatggag tagctaactc tatattagtt
     1141 aaagttaatc aaataggtac aataactgaa actttagatg ctatagaaat ggctaagaga
     1201 gcaggatata ctgctgttat atctcacaga tcaggagaaa ctgaagattc tactatagca
     1261 gatttagctg ttgcagtaaa tgctggtcaa ataaaaactg gtgctccatc aagaactgat
     1321 agagttgcta agtacaacca attattaaga atagaagaaa tggttggaga gcaagctaga
     1381 tattgtggat taaaatcttt ctataactta aaaaaatagt tattaaaatg gttactaaaa
     1441 aagattaatg aagagagaat ataagatatt taagctcatc ttataagata taaaagagat
     1501 aagtgttaat tgatttttca attcacttat ccttttttat attttattac ttaattttat
     1561 tcatagattc agaaaaaata tgaataaatg tttctatctc atttttatct aattgcatag
     1621 gaggttttta ttaaaaaata aaagtgaaga catattttga tttaatcata attgtcatta
     1681 aagtattatt gctaaatgtg gattatttat tttaaattat gttgaaatta aaagttatat
     1741 caatatttta aaatgattgt tttattttgg tggctatt
//
LOCUS       DACXMZ010000372         1754 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.373, whole genome shotgun sequence.
ACCESSION   DACXMZ010000372 DACXMZ010000000
VERSION     DACXMZ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.373"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(141..566)
                     /locus_tag="JD016_19200"
     CDS             complement(141..566)
                     /locus_tag="JD016_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAU5001729.1"
                     /translation="MARWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            complement(566..913)
                     /locus_tag="JD016_19205"
     CDS             complement(566..913)
                     /locus_tag="JD016_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001730.1"
                     /translation="MVSKTRKAIEMLYRYKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(907..1308)
                     /locus_tag="JD016_19210"
     CDS             complement(907..1308)
                     /locus_tag="JD016_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001731.1"
                     /translation="MGNNIIDDIEKRLESFGYILKDGDKWLIDFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLVAYLTAYGKNKILTFRCLRW"
     gene            complement(1318..1551)
                     /locus_tag="JD016_19215"
     CDS             complement(1318..1551)
                     /locus_tag="JD016_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001732.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKVVRYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(1551..>1754)
                     /locus_tag="JD016_19220"
     CDS             complement(1551..>1754)
                     /locus_tag="JD016_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HAU5001733.1"
                     /translation="AGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
BASE COUNT          514 a          346 c          179 g          715 t
ORIGIN      
        1 gataggatac tttgcttgga tttaaaggaa caataaaaaa acaaggttca ttaatacctt
       61 gctctacatc ctcactataa attgtatatt tttctccaaa tgatttatct aactttactg
      121 atattccatc tataatatta ttaagcatca aacactcctt taagtaatat taataacttt
      181 ttctctataa tcttatcaac ttggctttgt agttccatct ctgaaattgt taagaaatgt
      241 tgtcctttaa cccaaccttt tccatcttta gttctatgcc cgaaatttac ataacttgca
      301 tattcagtcg gattaacaac ctctataata taattatttc cttgtttata cacaggaagc
      361 gacctagcat aagccactcc attccatcct tgtcttaaga atcctgtatc aactggtgtc
      421 cttctaatta cttttcctaa gagtcttgct gctaattctc ttgctgcatc tttgcaaaac
      481 ttatctaaat caatctttgt aagcttctcc atctttttac aaactctttt aaactctcta
      541 aaatcaacac tgccccatct agccattatg ctttatcctt aaataactca agtattattt
      601 cttgatgatt tggatatata gctgattctc cacttcttac atactcttta tcatttataa
      661 taagttttga acctgcttta atttctatat ctggagatat aaagagttta atagtttgct
      721 ctagcttagc taattttcct tctgtagcag aaactatatt tttatatgaa agcttgcatg
      781 gttgattttc taatacaatc acttctttat tgttagttcg ttttgttaca gggtctttga
      841 ttggctgata ctcaactata gtacatttat atctatataa catttctatt gcttttctag
      901 ttttacttac catcttaagc acctaaaggt taatatctta ttcttaccat aagcagtaag
      961 ataagctact aagctatcaa agcgttgttc tggtgtttga gagccacttc ctatagcaaa
     1021 atctaccttt gtatcacctt ctgatataga cttttctaca gcttcaaagt taatgctttc
     1081 tatatctaat tgccccatat ttttcttggt aaataagaac tctccaacta tcatatcaac
     1141 ttcaatttct ttcaattcaa ttggcatagt ttttatatta caatctagtt taataatatt
     1201 ttctattttt tctcttacaa aatctattaa ccacttatcc ccatctttta atatatatcc
     1261 aaaactttca agtctttttt ctatatcatc aattatatta tttcccataa ttttcaccta
     1321 ctttttagta agtttatttt tctctttaag ctgtttattt tcttcttcta aagactcaac
     1381 ttttgacctt aaaatattat tttcagctat taaatctttt acatttaatg acttgccata
     1441 ccttactacc ttaccagttt catctatcaa atcatatccc atctctaaga aatcatctat
     1501 tttacactct tctatggtta atattctatt taatttcctt acttgtgcca ttatgctcca
     1561 gctccttcaa caacaaattg tattgcatca gcttttttat ttaatataaa tacatcctca
     1621 aaactttctt caaagtagaa gtattttccc tctgtaactg ctgttggttc gtctaactta
     1681 gagaactgat aagaaacagg tgtaattatt gcacttgggt gaactaagga cataaagatt
     1741 tgtttagctc ctgc
//
LOCUS       DACXMZ010000373         1746 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.374, whole genome shotgun sequence.
ACCESSION   DACXMZ010000373 DACXMZ010000000
VERSION     DACXMZ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.374"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            99..368
                     /locus_tag="JD016_19225"
     CDS             99..368
                     /locus_tag="JD016_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HAU5001734.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            394..>1746
                     /locus_tag="JD016_19230"
     CDS             394..>1746
                     /locus_tag="JD016_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HAU5001735.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDETDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
BASE COUNT          639 a          193 c          398 g          516 t
ORIGIN      
        1 aaaaagtggt ttactaacaa ataaatgaaa tatatgttaa cacatagttc aatttgtaaa
       61 aatataagat ttataaaaaa ataagggagg atattattat gaaagcaatt ttaacagtaa
      121 ttggaaagga taaaccaggt atagtggcag gaattagtga tgaattatac aagcaaaata
      181 tcaacatagt agatatatca caaaaaatat tacaggatta ttttactatg attatgatag
      241 ttgatttaga gaaatcatta aattcttttg agaaaacggt tgaggacttg gttgaaagag
      301 gtaagaaatt atcagtggat gtaaaaattc agcgagaaga aatatttaat tcaatgcata
      361 atctgtagta gacagtaagg ggaggtgata atagtgaatg taggaaatat acttgaaaca
      421 ataaagatga tagatgaaga acagttggat ataagaacta taactatggg gatttcttta
      481 ttagattgta tagaccctga tggagataaa gctagagaaa aaatatacaa taagattatg
      541 agttctgcta aagatttggt gaaggttgga aaagatatag agcgtgaatt tggtatccca
      601 attgtaaata aaagggtatc agtaactcca atttccatta ttgcaggtgc aacagatgaa
      661 actgactatg taaagtttgc acagacattg gataaggctg cagaggattt aggtatagat
      721 tttattggtg gattctcagc attagttcaa aagggatata caaaaggtga taagatacta
      781 attaagtcaa ttccaaaagc tctagcaagt actaataaag tatgtgcttc agtaaatgta
      841 ggttctacaa gatgtggtat aaatatggat gcagtgaaag aaatgggaga aatcataaaa
      901 gaaactgcag aattaacaaa agatgcaaaa ggttttggtt gtgcaaaatt agtagtattt
      961 tgtaatgcag tagaagataa tccatttatg gcaggagctt tccatggtgt tggtgaggct
     1021 gatagaatta taaatgttgg tgtaagtggc cctggtgttg ttaagagagc tttagagaaa
     1081 gtaaagggag agccttttga tgttgtttca gaaacagtta aaaagactgc atttaaaatt
     1141 acaagagttg gacaacttgt ggcaaaggaa gcttctagta gattggatgt tccatttggg
     1201 ataatagact tatcactagc tcctacacct gctattggtg attcggtagc aaatatatta
     1261 gaggaaatgg gtcttgaagt tgttggtact catgggacta cagcagcatt agcactttta
     1321 aatgatgctg ttaaaaaagg tggagtaatg gcatgttcac atgttggagg acttagtgga
     1381 gcgtttatac cagtatctga agatgcaggt atgattgatg ctgttattaa aggtgcttta
     1441 agtatagata agcttgaagc aatgactgct atttgttctg taggtcttga tatgatagct
     1501 gtgccaggaa atactacagc aggtacgctt ggagccatga tagctgatga ggcagcaata
     1561 ggtatgatta ataataagac tacagccgtt agaattatac cagccccagg atgtgatgta
     1621 ggtgatatgg tagaatttgg aggactttta ggacgagcac cagtaatgcc tataaataca
     1681 aattcatctg aattatttac tcaaagagga ggaaggattc cagctcctat acattcattt
     1741 aaaaat
//
LOCUS       DACXMZ010000374         1734 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.375, whole genome shotgun sequence.
ACCESSION   DACXMZ010000374 DACXMZ010000000
VERSION     DACXMZ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.375"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            106..1434
                     /locus_tag="JD016_19235"
     CDS             106..1434
                     /locus_tag="JD016_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAU5001736.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          585 a          204 c          318 g          627 t
ORIGIN      
        1 attttatttt aataaataat aaagctattt ttctatatat tggcataata tatgcttatt
       61 taactatgaa attaaaatta aataggatta atgaggagga aaaaaatggg aagtaatgaa
      121 ctgaaaaaga ctcttggcgt atcagcagca ttatcaactg ttgttggttc cgttatagga
      181 gcgggcgtat tttttaaacc acaagcagtc tatacattaa ctggaggagc tccaggtctt
      241 ggtatattgg catggttaat agctggaatc ataactataa cagctggtct tacagctgca
      301 gaggtatcag ttgcgatacc aaaaactggt ggaatgatgg tatatataaa agaaatttat
      361 ggagaaaaat taggtttttt aactggatgg atgcaaattg tattatttta tccagggatg
      421 atggcagcat taggtgttat atttggagaa caagcatcag cactaattgg tagtccatca
      481 cttttacttc caatagcaat tggaattata gtaatagttg cagggcttaa tatgttaggt
      541 tcaaaaactg gaggagtcat acaaactgtt tctacaattt gtaagttaat accacttatt
      601 ttaataatgg ttgttggatt tattaaaggt ggtggaaata atccaatact tacacctatg
      661 gttggtgaag gattaagcct gggtagtgta ctaggtcaag ttttaattgc tatattattt
      721 gcttttgatg gatggatgaa tgttggtact ttagctggag aaatgaagaa tcctggaaaa
      781 gaccttccaa aagctataat aggtggattg tcagttgtta tggctgttta ttttataata
      841 aatttagcgt atctttgggt attaccagct aatgaattag caaattatgc ttctcctgca
      901 tcagcagttg ctgaagttat atttggttca atgggtggaa aaataatatc tgtaggtata
      961 ttaatttcag tgtttggagc attaaatgga tttttactaa ctggttcaag ggtggcatat
     1021 actttagcta cagataagac tttaccaaag tatggtatat tttctaaatt aaattctgct
     1081 caagttccag caaatgcaat agcattagta tctgttattg catctatata tgctttgtca
     1141 ggtcaattta atttattaac tgacttagct gtatttgcta cttggatatt ctatgtatta
     1201 acttttatag gggttatgaa acttagaaag actcatccaa atataccaag agagtataaa
     1261 gttccattat atcctattgt tcctataata gctatagcaa gtggtatatt tgttgtagtt
     1321 aatcaattgt gttttgcagg tatgaaaact actatgatat ctataggagg tcttgtaata
     1381 acagctatag gcttaccagt gtatgcttat atgactagag gagtaaaaag ataaaccaag
     1441 tagtttacaa atgcttcata attaaaataa aagagactgt ctcaaataga gataagtctc
     1501 ttttttattg ttaaaatgat aaagatatgt ttaattttac aaaaaaagag cgttccatga
     1561 actaaaaagt ttattttaag gtattcattt tatttttagt tttttgatta taaaattaaa
     1621 catctaaaaa attatttatt ttaaaatact ttattttaaa gtgtttttta tcaaactaag
     1681 tggtatctgt acaacaaata ttatacatag cttttatgaa aattaaatct ttct
//
LOCUS       DACXMZ010000375         1728 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.376, whole genome shotgun sequence.
ACCESSION   DACXMZ010000375 DACXMZ010000000
VERSION     DACXMZ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.376"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            113..1603
                     /locus_tag="JD016_19240"
     CDS             113..1603
                     /locus_tag="JD016_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAU5001737.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
BASE COUNT          664 a          183 c          351 g          530 t
ORIGIN      
        1 tatattcgat aaaataaaat ttaaacatat aatattttat gtatatatta ataaaaggac
       61 atattgtgtt ataatggtat cgtataaaat catttacggg ggtggtatta aaatgtttca
      121 atttgaaaca caaatacaca aattaagaca tgatgtttta actgcaatag ctagacttgg
      181 aaaagaagat aaccttacca gagaagcaat ggataatatt cagtatgaaa taataaaagg
      241 tgagaaacct acatacagat gctgtgttta taaagaaaga gctataatat ctgagagagt
      301 aaatgtaagt atggggttac aaccaggtca agataagata gatttagata caatggatat
      361 agaagaagat gaacaaatta tatatatttt ggaagctgca tgtgacagtt gtccaataaa
      421 tgaatttaca gtaacagata tttgtagagg atgtctagca catagatgta aagaagcttg
      481 taaatttgga gctataacac atgtcggagg aatggcctat ataaatcatg aattatgtaa
      541 ggcttgtgga atgtgtaaaa aagcttgtca atatgatgcc atctcagagg ttgttagacc
      601 atgtaagagt gtttgtccaa ctaatgcctt aggatttgat agagaaaata tgaaagctat
      661 gatacatgag gataaatgtt taaactgtgg agcatgtatg tcagcatgtc catttggagc
      721 aatatctgat aagagtttga tagctcctgt agctagaaag ttagttcaaa aagagaaaat
      781 gtatgcggtg gtagctccag ctataacagg tcaagttgaa gcaaatgtta catatggtca
      841 aataaaaaat gctataaaat cactaggatt tgttgatgta ttagaagcag cttgtggagc
      901 agatgcagtt acagttcatg aaagcttaga atttgctgaa agaatggaaa agggagaagc
      961 ttttatgaca aattcttgtt gtccaggatt tgttggttat atagaaaaaa catacccaaa
     1021 tcatgtaggg aaaatatctt ctacagtatc tcctatgata gctagtggta gatttataaa
     1081 aagtcatgaa ccagatgcta aagttgtatt cataggacca tgtacagcca aaaagagtga
     1141 ggcagctaag aaagaattaa aaggagctgt tgattatgta cttacttttg aagaaataat
     1201 ggcattattt gaggcatttg atatagactt ggctaagtgt tcaaatgaag atatagatga
     1261 tgcatcaatt tttggaagag gttttggagc tgctggtggt cttacaaaag ctatagaaaa
     1321 ttatcttgct gaaaaaggta ttggtgttaa ttttgaacct gtaaagattt ctggttcaag
     1381 agaaatcaag aaaactatga ctatggctaa tttaggaaag ctagaaggta attttataga
     1441 aggaatgatg tgtgaaggtg gttgtataaa tggttctggt aaattcttat caggtgcaaa
     1501 ggttaaagcc acttttgata gaaaaaattc tcaatcaact aagaaaagtg tcttagcaaa
     1561 tacaaagata aaagatttta gtaatataaa tttagaaaaa taaagataat acaaagcaac
     1621 aaaaatacat attgttaata gagttgttct aagttgaggt ttaacttttg gacaactcta
     1681 tttttattta tatatttaat tattgaacaa ctaaaataaa aattatat
//
LOCUS       DACXMZ010000376         1721 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.377, whole genome shotgun sequence.
ACCESSION   DACXMZ010000376 DACXMZ010000000
VERSION     DACXMZ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.377"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(258..515)
                     /locus_tag="JD016_19245"
     CDS             complement(258..515)
                     /locus_tag="JD016_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAU5001738.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            complement(530..670)
                     /locus_tag="JD016_19250"
     CDS             complement(530..670)
                     /locus_tag="JD016_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001739.1"
                     /translation="MRNFIKVNTKINFKAKKLFIRFNKTNKYSLVNNVLNKNFVKSIK
                     IS"
     gene            1095..1316
                     /locus_tag="JD016_19255"
     CDS             1095..1316
                     /locus_tag="JD016_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HAU5001740.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
BASE COUNT          663 a          239 c          161 g          658 t
ORIGIN      
        1 atttttcccc tttatacaag caattgtatc attttatcta ttttttagcc aacaaatatt
       61 ttcgaccatt ttctttgtat tctcaatata tctccagtat ttaacaaatt tttttataga
      121 atatacaatg tatttaaatt aataagtagt aaatttagtt aatttgtcta aaaaaacttt
      181 ataaaacatc ttctttgtct ttactcttac gtttaacaag gctattttca ttatgtttaa
      241 acattatttt attatattta aacactattt tcattgagta ttcgcttaat attgcttaca
      301 cacatattat atctaatagc caattcattg tggttgactc cattaaattg ttttactata
      361 tccctatttc tagcatatct aaaaagcccc ttatacgtag gtatataagt atttgttcct
      421 ccatacaact tggcaacctc tatgaactta tcaattccga ttatttctac cattgcatga
      481 aattgaactg gtatatcctc cacttttatc ttcataagtt acacctcctt taagatattt
      541 ttatagactt aacaaagttt ttatttaata cattattgac taaactatat ttattagttt
      601 tattgaatct aataaataat ttcttagctt taaaattaat ttttgtatta actttaataa
      661 aatttctcat aacttataac tttgttagct ttaataaaga ttttaatggt ttaaaagtaa
      721 aatgttaata ttggactttt aactttttta tttacagatt aaatttaata aaatttttaa
      781 cataagcttt aacatttaat ttaacataaa ctttagtacc taacatttaa ctctacacat
      841 atgaaaatcc atacaaaact atctcaacta agcaggaata tatacaaata ctatttcaaa
      901 acactaagaa catatcttca caaataactt gtaaactttt tcatcatcaa aaaagtaaaa
      961 tttttcctat aaaaatttac acaaaaaatc tttagaaatt actgtttaac tccgatatta
     1021 tcttagatga tagtattata gctaacaaag aaagtcgaca aatctttgaa attaaatttt
     1081 taggaggtaa ttttatggaa gttgtttcaa caaaaaattt atctagtata aaactaaaat
     1141 tggatgctgg ttttgatgat aagggtaagg cagttgttaa aagtaaatca tttgcaaatg
     1201 taaaagcaga ggcattaaat gaagatgttt atgctgttgc agaagcaata gcaagtcttc
     1261 aagagaaccc tattgtagat attttaaaat tagatagtac atcattatct aaatagtacc
     1321 ttttaaacgt actctgcttt acaaattaaa aaaattaact ctttatataa aagattaagt
     1381 tatataacgc acttaatacg ctatagattt ggcagattat aacttcaatt ttgattataa
     1441 ctttaatata ttaatgcttt gaaaaattaa tatattaaac gtatttcaac ttaaacatgt
     1501 tagctatttt agatgctacc ctactttaat ctaattaatt gaaataaatt aaatattttc
     1561 tatatcagca ataactagag tcaacaatta aaatactaat attgacaatt gaaacactga
     1621 tactaacaag caaaacacta atattgacaa ttgaaatatt aatactaaca agcaaaacac
     1681 taatattgac aattgaaata ctaatactaa caagcaaaac a
//
LOCUS       DACXMZ010000377         1721 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.378, whole genome shotgun sequence.
ACCESSION   DACXMZ010000377 DACXMZ010000000
VERSION     DACXMZ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.378"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            181..621
                     /locus_tag="JD016_19260"
     CDS             181..621
                     /locus_tag="JD016_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HAU5001741.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(809..994)
                     /locus_tag="JD016_19265"
     CDS             complement(809..994)
                     /locus_tag="JD016_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001742.1"
                     /translation="MNKNSNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKK"
BASE COUNT          658 a          246 c          170 g          647 t
ORIGIN      
        1 tttacatttt tttccaatat atataaatat catatttttc tattagctaa aactaattac
       61 tttatatttc tacgtattag tataataata cataaaaact tgaataatgc ataaaaactt
      121 gacatactgt tgtcataatt actttgctat aattaaaagt aaaaatggag gtaaattcta
      181 atgtatgaac gtatgcttga taaaagcaat aaaccctgtt tgtctcaaat aatagaatat
      241 ataggtaaag atggatatgc acgattgtgt aagttggaaa cttacttgaa aactcaatat
      301 aatttatcaa gagaacttcg atttcctttt ggaaacaatt atggttgggg ttacaaatat
      361 agccataaat cttttcattt attttatgct ttttttgaaa aaggtgcatt tactgttctg
      421 attcagatag gtgacaagca agttccagca gtagaaaaaa cgttggtagc tttgacacca
      481 aaagctaaag agctctgggt cactcgttat ccttgtggca ataatggagg ttggattaag
      541 tatcgtattt tagaagatgc agacttactt gatattattc aatatataag agcaaaaaaa
      601 tctccaccta aacaaaatta acttttattt tttgtgattt aaatatagtg tatataaatt
      661 ccattctttc catataaaaa aaataatata aaaaatatct attagtataa cttatttagt
      721 atgtcttatt ctacttttta cagataatta aaaagtttta tcataaataa atcataacta
      781 gacatcctag taaatcaaaa ttaatttatt attttttatt agaatttttg ataatagtcc
      841 ctatacatat gccaattatc attccaaaaa ttagaccata acacattgtg atacctacat
      901 ttcctttaga aatacctata gcacaaccta tagccattcc aagtatcatg cctataaaca
      961 tacatataat aactaaattg ctgtttttat tcatagtttt tctccattca atatatttta
     1021 aattgcctag ctcgccttct tagcttaaaa caaagaaata taaattaact ttctttcatt
     1081 ctaatatctg cttgtataaa tgtcaaacaa cacatttctt gtcataacct cctctttttg
     1141 aagtaagtat aatctcccag acacaataat atcaatttcc taaaaccatt taagtaactt
     1201 ttatatttac ataatattta caatcataaa ctatattgtt attcatttat acatcataaa
     1261 cactccctcc taatcattct tatacttaaa ataatgttca tttataataa tatatctaac
     1321 gcactttaat tgaaaaaact aaaactttga ctttcattcc catatataaa taagtatact
     1381 ttttgatgct ctattacaaa tcaactaaat taattgatat aaattatcat tttctgttga
     1441 aaataccatt cgcacatgct atacttaatt acataaatgg taataaatgt catatgattt
     1501 tacaaatcta ttaataacaa ttattacttt tactgtattt taaatttttt ggactaaata
     1561 tcaagaactt tctaaaatac aaagtacaaa aaatttatat aagttataaa tttatatgaa
     1621 ctagataagc tcaaacaata tgttatgtat aaactgtgta gtattcggtt ataaatttat
     1681 ataaattata taagctagat aaagtattac ctcaactata t
//
LOCUS       DACXMZ010000378         1677 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.379, whole genome shotgun sequence.
ACCESSION   DACXMZ010000378 DACXMZ010000000
VERSION     DACXMZ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.379"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..199)
                     /locus_tag="JD016_19270"
     CDS             complement(<1..199)
                     /locus_tag="JD016_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001743.1"
                     /translation="MGIFIFLKLNCKYYCVIYILILNVCFIRNKLIEKIVLTMKAKKV
                     LMKIKNFIVPFYIFFYIFYYAL"
     gene            complement(379..1086)
                     /locus_tag="JD016_19275"
     CDS             complement(379..1086)
                     /locus_tag="JD016_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAU5001744.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          541 a          287 c          150 g          699 t
ORIGIN      
        1 ttaaagcata ataaaatata taaaaaaata tgtaaaatgg tactataaaa tttttaatct
       61 tcatcaatac cttttttgct ttcattgtta aaactatttt ttctattagt ttatttctta
      121 taaaacaaac atttagtatt aaaatatata tcacacaata gtatttgcaa tttaacttta
      181 aaaaaataaa aatacccaac tgacctaagc aactcgattg ggtataaata atctaaaaaa
      241 tcatttttgt ataaactctt tatttaactt acatttttat ttaccttcta ctttaattca
      301 acttatattt ctatgcttct tatactctca ttcaacttat atttctactt atcttatact
      361 tttataaatt catcacaatt atttagttct tgctatcaaa gaaattgtat taaaatactt
      421 tccattctct acttccatca tttttttgtc atttatagca tatgaattat cacaatttat
      481 ccaatccttc caagcttcat catgacaatt taaagcttca catttctcta caatcacaga
      541 ctccgattca gaccaaagtt tattccacca tgtaattgaa tggaagttca tattatcttg
      601 ccaaaatgga atgagttcat ctggtataca atccacgaaa tcttctttta atccaggcat
      661 tgccatagca agtattcccc cctcttttac aagaggtgat atatgattgt ctaaaaatcc
      721 ttctttgttt ccaaaatagt gataagaatc gatacttatc accgcatcaa agaaaccttc
      781 tgcataaggt agttcatgtg cctctgcctg tattggaaat attttatcat ccaatttaaa
      841 ctctttaaat cttttatagt tttcagttgg ttctatccat aaatctgttg caaatacagt
      901 agcatcaaat tcctttgcta aaaatatcga agatatacct ttaccacaac ctaagtctaa
      961 aatacgcatt cccttttcta gttttatctt acttgttaat tcttccaata tttttataca
     1021 attaggtccc atcatatttt tcatgagaaa gtccttatcg tatttattac tctttgtaaa
     1081 tatcattcta tctttccttt ccctatcagt tattatttat taaattttat acgtttgatt
     1141 ttttattttt tatatgcttc ttatattgag caatctcttt tttattcatt ctttaacaga
     1201 caattttaat gtgtaatttt atccactctg tgatagtaac tttaatgaat aatcttatct
     1261 actgtgatag ttaactttga tgggtaattt tatctactct atgataatta ttttagtgag
     1321 taattttatc tactccacag caatcgcttt aatgagtaat cttatccact ctatgataat
     1381 tattttagtg agtaatttta tctactctac agtaatcgct ttaatgagta atcttatcca
     1441 ctctatgata gttattttag tgagtaattt tatctactcc acagtaatcg ctttaacgag
     1501 taatcttatc caatctataa tagtcacttt aatgagtaat cataattttt atatcatttt
     1561 agatacttac tctaataaga agtcattttc taatacttaa taagttttaa ttacttccac
     1621 tttaacctat atactaatta acttatttaa tttagtgcat atttgacact ttacctt
//
LOCUS       DACXMZ010000379         1636 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.380, whole genome shotgun sequence.
ACCESSION   DACXMZ010000379 DACXMZ010000000
VERSION     DACXMZ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.380"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(88..1479)
                     /locus_tag="JD016_19280"
     CDS             complement(88..1479)
                     /locus_tag="JD016_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAU5001745.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
BASE COUNT          588 a          308 c          182 g          558 t
ORIGIN      
        1 caaagaggaa attacattta attttatatt taaatgtaac ttcctttatt taaagtaatt
       61 catttattta caataattcg tcatgaatta tttttctttt gttagtaaat ccatagccat
      121 taatcttgtt aatacatttc ttattcccaa taatattaaa ccaaatatta ttaaaactgt
      181 tgctattcct ccaaatatct gtaaaaaacc aagtgtggct gtaaaaccag caatctttcc
      241 tgataataaa cttgcaaaaa aacttgttag ataccaggct cccatagaca acgtagcata
      301 cttagctgga gctaatttat tgaacattgc aagtcctata ggagataaga ataattcacc
      361 tactgttgta aatatataag tcaatactat aaacaatata ctagccttta ccattccatc
      421 tggaccatta gctaatgtag caaataccat aactataaac ccaacaccca ttattatcat
      481 tcctaatccc attttaactg gaatagagaa atcacctctt ttggtatgag ataatttaac
      541 ccatagggta gctaatactg ggcataaagc cacacataat atagcattta gtgatgtaag
      601 ccatgctact ggcatttcaa atccaaatag agttctattt acattatccc ttacataaat
      661 cataaaagaa ctttgtgttt gatagtatgc tgtccagaat aagaccacaa ataaaaataa
      721 aataaccatt gctattatcc tatttttctc caacttagta agtggtccat actcaatttt
      781 ttcaccttta ttacctttag tcacaggata ttttccgact tcatttaata attttggtgc
      841 aagaagtgta aatataatca tacctaacat catgcctata ccacaaatta aaaatgccat
      901 cttatatcca taagatacta tatctccact tgctcctctt actgcaaata cattatcaga
      961 aagcaatcca catattattg gagctataaa tgctcctaca tttactgcca tatagaataa
     1021 actgtatgct gcatcttttc ttgacacatc agttttagga tatatatccc caactatatt
     1081 tgagcaactt gatttaaaaa aaccatttcc taaaataatt attgatagac ctacccaaac
     1141 cattgcaata gatgtagatg aagcaaatag tacgaaatct cctattgcta ttaaaagtgc
     1201 acctatagaa atacattttt gttgacctaa aactttatct gctaaccagc caccgactaa
     1261 tggtgtaaga tatataagac ctgtaaataa gccaaacatg gatgttgctt cttgagtaga
     1321 aagacctaaa ccaccttgag ctacacttgc tgtaaaaaat aataccaata aagctgttaa
     1381 accataataa ctaaatcttt cccacaaaat tgtcatgcaa ataagccaaa aactcttcgg
     1441 atgaccactt tttttagata cgctttctgt acttgacata aaaaaacgcc cccttttgtt
     1501 ttatagttaa atttaatatt aatattgctt taaccatttt agcacatact tataatatca
     1561 tcttttctat gtttactcaa tagattatcg cctgtatttt cattttttta ttattatttt
     1621 taaaattatg aaaaat
//
LOCUS       DACXMZ010000380         1609 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.381, whole genome shotgun sequence.
ACCESSION   DACXMZ010000380 DACXMZ010000000
VERSION     DACXMZ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.381"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            97..1533
                     /locus_tag="JD016_19285"
     CDS             97..1533
                     /locus_tag="JD016_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HAU5001746.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          655 a          166 c          274 g          514 t
ORIGIN      
        1 taatttgcaa ttttacataa gtttgttaag tacataacta atttaagaat atgtatttat
       61 atattaaaaa tttattatga gggggacaat tttattatga aattctataa aagaatatta
      121 acattgactt taagtatttc ttttgtattt gcaaatattc aattagtaga tgcaatatca
      181 tctgtagaaa aaatacaagg aaaaaataag tatgaaatcg caggtaaaat agcagataaa
      241 aatgcatata aaactgctat attaattaat actagtaact ctattgcaga tggattaagt
      301 gcaagtggac tagcaggagc tttaaatgcc cctatactat taactgaaaa aaatactata
      361 ccaactgaga catctgcaag attaaaaaat gtatcaaaag tttatataat tggaggaaca
      421 tattctatta gtacatctgt cgaaaattca ttaaaatcta agaaaatgaa agttgtaaga
      481 attaagggaa atgacagaat aaaaacaagt tataatgttg caaaagaaat aaattcaata
      541 aaaaaagtca atactgttat gctaaccaat gcatataaag gagaagcaga tgctataagt
      601 attgcatctg tagcagcaag agataaagca ccaataattc ttactaatgg acaaagtata
      661 cctttttcaa ctagtggttt aaagtcttat gtaataggag gaacagcttc aatgagtacc
      721 actttagtaa acaatactaa atctactaga ctagggggga gtactcgttt tgaaactaat
      781 aaagcaataa taaataagtt ttataaggat gctagagagt tttatatagc tggagcttat
      841 gaattaacaa atgcacttgt aggatcttct ttatcaaaac atggtccaat ggttttagtt
      901 aatgatggta gcaataaaag cattttaaaa aatgctaaga agataacttc tataggatat
      961 atagattcaa atatagtaca acagtgttta aatataacaa atggaattgg agatattaat
     1021 actggggttg taaaaaatgt taaacctact acaaagacta taaaagatgg aatgtataag
     1081 gttggaaaag atatctcagc aggtgaatac ttaataactt caaactcagg aagttatgct
     1141 tcttattatg aagttacaag tgatagtaca ggtaatgctg atagtatact ttcaaatgat
     1201 atttttagtg ggactagata tatcacctta aagaatggtc aatatattaa aattgaggat
     1261 tcaacaatga cattagcaaa gtatgctaaa gcccagaagg ccaagaatgg taagtttgga
     1321 aatggtatgt ataaaattgg attagaaatt cctgctggag aatatattat aatgtcaaat
     1381 tcttctgatg catattatga agttagaaat gatagcttag gtaatgcaga gggaatagtt
     1441 actaatgata ctttttcagg aagaagatat ataacggttg aggaaggtca atatttaata
     1501 ttaaatgatt gttatttaat tgaaaatgaa tagatgtaat tgtatttaat agtaaatcat
     1561 atattttatt taaggaaagg tgttttaagt tttaatttaa aacatcttt
//
LOCUS       DACXMZ010000381         1580 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.382, whole genome shotgun sequence.
ACCESSION   DACXMZ010000381 DACXMZ010000000
VERSION     DACXMZ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.382"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            49..783
                     /locus_tag="JD016_19290"
     CDS             49..783
                     /locus_tag="JD016_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAU5001747.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGDFAFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRRSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(926..1417)
                     /locus_tag="JD016_19295"
     CDS             complement(926..1417)
                     /locus_tag="JD016_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAU5001748.1"
                     /translation="MNNQQQEVYKKLDELNIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMNKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          585 a          189 c          235 g          571 t
ORIGIN      
        1 tttagtagag tgttgctaag tgaatatatt taaaatggag gaaactttat gaaacaaaat
       61 aaatatgatg atgatagatt ttttaataaa tacagtaaaa tggaacgttc tacaaatgga
      121 ttagcaggtg ctggagaatg gcatgtttta aaaaaaatgt tacctgattt caaaggaaaa
      181 agagttttag atttaggatg tggatttgga tggcattgtc aatatgcagt tgagaatgga
      241 gcaatttctg ctgttggagt tgatatttca gaaaaaatgt taaaagaagc tagaaacaaa
      301 acgcaacttg ataatataaa ttacatatgt atgcctattg aagatattaa ttttccaaaa
      361 gatagttttg atgtagttat tagttccttg gcatttcatt atattcaatc atttgaagat
      421 atatgtaaga atataagtaa ttgtctttca aataaaggtg attttgcatt ttctgttgaa
      481 catcctatat ttactgcata tggaagtcaa gagtggtact gtgataaaga tggcaataat
      541 ctacactggc cagtagatag atattttgaa gaaggagtac gtaggtcaaa ttttctagga
      601 gaagaggtta taaaatatca taaaacatta acaacttatt taaatacgtt aatgaaaaca
      661 ggatttgaaa tattagaaat tattgaacct caaccagaag aaaaactgtt agatacaatt
      721 cctgatatga aagatgagct acgtagacct atgatgttac ttgtatctgc aaaaaaaaga
      781 taagtaaata taaataatca ttattgtaga aaaaaagttg tagaaaataa ttatactaat
      841 ggagatgatt ataccagtgg tattaattac atcagtagta aggaggcaaa agcattcgtt
      901 gcttttgcca ttttttaaat tgcaattata cttctacgta gtatatttcg tttccatttt
      961 cttctataag ctttttcata tcttcaaatt ttaaaaatac agtagcagta ttatcatttg
     1021 gatgtatacc tattttattc atttttaata aatccttatc aaatacaact tttacaacag
     1081 cctctttatc atttaatata ccaaaaggag tcacagctcc tttagtaagt ttcaagtatt
     1141 tttgaagtct ttcttctgat gcaaaactaa gaggtttact atcaatttgt tgacgaatat
     1201 cttttaaatc tgcttttttg tctttaccta atacaactaa aaaatgttct tttcctttag
     1261 catcacgaag aaataggttt tttacaacaa attccatatg taatgttttt aattcttcca
     1321 tttcttcaat tgtataaact ggtggatgat ttacagcctc ataaggtatg tttaactcat
     1381 ctaatttttt atatacttcc tgttgttgat tgttcatgat ttcctcccat tagaaattta
     1441 ttttatatat aaataatatt atcacagaaa actttaaaag aaaacgtttt atataatata
     1501 gacaatagat tatattttta taaagaagca agaatactca ataaaactta aagaaattaa
     1561 acaatttcta aaaatacctt
//
LOCUS       DACXMZ010000382         1577 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.383, whole genome shotgun sequence.
ACCESSION   DACXMZ010000382 DACXMZ010000000
VERSION     DACXMZ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.383"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            134..1537
                     /locus_tag="JD016_19300"
     CDS             134..1537
                     /locus_tag="JD016_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HAU5001749.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT          658 a          183 c          306 g          430 t
ORIGIN      
        1 gtatattgtt tgagttgtat ggataactat ggtatcatat ataaaatatg gtaacaaaat
       61 tgtaactttt gattaaaaat aagtatgagt actaataaaa aatatgaaaa gtacattggg
      121 ggtagaaaat aatatgaaga atagtaaaaa gatattagct ataggactta cactattttt
      181 agtaatggta aatactccta tggtaaatgc gttgacatca gttgagcaaa taaaagggaa
      241 tgacagatac gagacagcag caaaaatagc agataagcaa aactacaata cagcaatact
      301 aatcaactca gataatagct tagcagatgg tctaagcgca agtggtttag ctggagcctt
      361 aaatgcgcct attttgatga caaaacaaaa ccagattcca aacacaacta tggaaagatt
      421 aaacaaagca aagactgtgt atataatagg ttcagaatca acaataagta aaaatgttga
      481 gaatcagtta ctatccaaaa agaaggttgt acaaagaata tttggtgaaa atagatttga
      541 tacaagtata aaaatagctg aaaaaatcaa agaaattaag ccaatagata aagtaattat
      601 agccaatgga tttacaggag aagcagatgc gataagtgca tcaccagtag ctgctagaga
      661 tggagtacct ataatactta cagatggaaa tagtgtggga tttgacacga caggcttaaa
      721 gagttatgcg cttggttcta gtgaaataat cagcgacgaa ttagttaaga gcacaaattc
      781 tattagattg ggtggcacag atagatttga aactaataag atagttatac aagagtttta
      841 taaaaatagt aaagaatttt atctaagtaa aggcttacaa ttaacagacg ctttggctgc
      901 ctctacaata gcaaaaaatg ctcctgtagt tttagttgaa aatggcagta acaagagtat
      961 tttaagtggt gcagacaaat tgactgtact aggaggcata aatcagaatg ttatcaagca
     1021 atgtataaat caagcttcac caaatcaaca aggtttatat tacaatccaa atgatagagc
     1081 atttaaagag agaataaagg gtaaagtata tgctcttaca aagcagtata gaaaagagaa
     1141 tggagtaaga gcgttatctg tagcaagcag attagagggt ctagcaaatg attggtctaa
     1201 tttaatggca aataagaaga ctttatctca tacgataaat ggaaaaaatt catattctac
     1261 ttttttgaaa tacttagatt ggagtgagat taaacctgga tatatagcag ttcaaggtga
     1321 aaatataatt aaatataaaa ttccagataa acctgtatat acaaatagag atgcagatga
     1381 tataggaaac tttattttta atgagtggaa gacaaatcca gaagaaggca ctaatatgtt
     1441 gcataaaggg tatgaaataa tgggatttgg tatagctata acaggagata agaatctata
     1501 tgctacacat gaattttatg gaagatataa agaataagaa aatatacaca aaattatgct
     1561 tgtaaggaag ctatata
//
LOCUS       DACXMZ010000383         1574 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.384, whole genome shotgun sequence.
ACCESSION   DACXMZ010000383 DACXMZ010000000
VERSION     DACXMZ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.384"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            173..874
                     /locus_tag="JD016_19305"
                     /pseudo
     CDS             173..874
                     /locus_tag="JD016_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
BASE COUNT          614 a          170 c          205 g          585 t
ORIGIN      
        1 ttgactaaac ttattgacta ataaattaga tgaaaagttg agacttactg aaaataaata
       61 caatgaaata tatactacaa gtattaaatt aaatctgaac tttacatact attagaataa
      121 gtattataag attataatga tttaaaaata ttaaaataat tgaggtaata acatgaacaa
      181 ttccttttta gataaattga ttgagacaaa agaacttaaa aattcacttt ataatgtgtt
      241 aaaacacaat tttctttatc attctaataa gatagagggc agtactttta ctactgaagc
      301 attagcactt ttactcgata agaatgttgt tacaggaaga catactttag atgatgtaca
      361 agaaactgtt aattctagtt atgtatttga tacaataatt gactcactga aagagaaaat
      421 aacacataat ttcttaagga atctacattc aagtttaatc ttcaatacaa ccctacattc
      481 caggtagatg tctggtatat ataaaactat acccaatatg atactaggaa ctgatgtaag
      541 catagcacaa ccatttgaag ttgagccaaa gcttgatgaa ttaattgaat ggtactatag
      601 tcagagtgaa gtatctatta aagttatagc tgaatttcat tatagatttg aattgataca
      661 tccatttcaa gatggtaatg gtcgcatagg tagatttgtt atgctaaaac agatgttaga
      721 aaataatctt cctataaaaa ttgtatcttg ggatagtgag gaattgtata gaaactcctt
      781 aaattcttgt agtttaggta attatgtccc tttaatcgaa tatttatcaa gtttagaaga
      841 ttttagagaa gtatataaaa tgttatggaa ataattgttt tcagcaaaaa aagtttttat
      901 ataaatatat ttttatctat gctatttaac gctgatttct tagttattct tagcttggac
      961 tatatccact attttaacta tataaaatag tggatatagt ttaactatct ctttataact
     1021 atttttatgt atcttagttg tttcttaata tggggacagt tcataaaact aaaagtgatt
     1081 tttaatttta tcatttgtaa tttaccatta catcagaaaa atgcttatca ttagaatttt
     1141 cataaaaggt acctattttt ttcaattaac taaatcaatt ctattagtct taatctttcg
     1201 tatttcaaat tgataatata agaaaataaa aaataaaaaa ttttaaattt tttatttatt
     1261 gcgaaaaaaa taaaaaggtg ctatactttt tgtaacatat tattcaaatt attaagagat
     1321 agtaaacatt tatagtttaa tttgtagtac actagcatga tttgcagaac aatgaattaa
     1381 tctatagtat aatagtttaa tttaaagtac aacgatttaa tctatagtac aataacttga
     1441 tttgaagtac aacgatttaa tctatagtac aataacttga tttgcaatac aatgaattaa
     1501 tctatggtat aatagtttaa tttgaagtac aatgaattaa tctatagtat aatagtttaa
     1561 tttgaagtac aacg
//
LOCUS       DACXMZ010000384         1549 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.385, whole genome shotgun sequence.
ACCESSION   DACXMZ010000384 DACXMZ010000000
VERSION     DACXMZ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.385"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..285
                     /locus_tag="JD016_19310"
     CDS             <1..285
                     /locus_tag="JD016_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAU5001750.1"
                     /translation="QSSGDVAKESLKNNIGLKFAFRSTDLGEIKQTLEFFGIDKDDEN
                     NQKRLRDLENGQCLLQDLYGRVGVVQIHPVFEELLHAFDTRPPVQRNEVE"
     gene            285..>1549
                     /locus_tag="JD016_19315"
     CDS             285..>1549
                     /locus_tag="JD016_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAU5001751.1"
                     /translation="MKERIKGAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINEENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTQNGFSSSGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGADRVVALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISKDYPFFMVAFLQIVCFAGI"
BASE COUNT          428 a          320 c          368 g          433 t
ORIGIN      
        1 caatcttctg gggacgtggc aaaggaaagt ctgaaaaaca atatcggctt gaaatttgca
       61 tttcgtagta ccgacttggg cgagataaag cagaccctag aatttttcgg cattgacaag
      121 gacgacgaaa acaaccagaa acgcttgcgt gatttggaaa acggacaatg cttgttgcag
      181 gacttatacg ggcgtgttgg tgtagtacag atacaccccg tctttgaaga actgctacac
      241 gcctttgata ccagaccgcc cgtacagaga aatgaggtgg agtgatgaaa gaaaggataa
      301 aaggtgcgtt cacaaaaagg aagatacttc actttctcaa aatggctctg ttcgtggtgg
      361 cactctccct tatcctgctt tcacttttgg ggacggtggc tcatgcgacg ggacttgtag
      421 acgataccat aaacgaagaa aatctttact caaaatatcc gctgtcgaat tatcagcttg
      481 atttttatgt agataatagc tggtcgtggc ttccgtggaa ctggctggac ggtatcggaa
      541 aatcggtgca gtacgggctt tactgtatca ctaattttgt ctggacgata agcctttatt
      601 taagcaatgc cacgggctat gtagtgcagg aagcctataa gctggacttc atcaacgata
      661 tggcagacag tatcggaaaa agcatacaga cccttgcagg cgtaacacag aacggttttt
      721 ccagctctgg cttttatgtt ggtttcctgc ttctcattat cttagtggtg ggactttatg
      781 ttgcctatac gggacttatc aaacgggaaa ccagcaaggc acttcacgcc gttatcaact
      841 ttgtagtggt gttcgtactg tccgcttcgt ttatcgccta tgctcccgat tacatcaaga
      901 agataaatga attttcatca gacatcagta ccgcttcact tgatttggga acaaaaatca
      961 tgctccccaa ctctgacagc gagggcaagg acagcgtaga cttgatacgg gacagcttat
     1021 tttctattca agtggaacag ccgtggctac tcttacaatt cgggaacagc aacgcagaag
     1081 aaatcggggc agaccgtgtg gtcgctcttg tatcggcaag tccagaggac gaggacggga
     1141 aaaccagaga ggaagtcgtg aaaacagaaa tcgaggacaa cgacaacaac aatctgacga
     1201 taccgcaggt ggtaaaccgt ttaggtatgg tgtttttcct cttgttcttt aacttaggta
     1261 tcacgatatt tgtattcttg cttacgggca tgatgttgtt tagccagata ctttttatta
     1321 tctttgcaat gtttttacct atcagctttt tactttccat gataccgagc tatgaaagca
     1381 tggcaaagca ggcaatcgta agggtgttta ataccataat gacacgggca ggcataacgc
     1441 tcattgtaac ggtggcgttc agcatttcca gtatgtttta taacatctcc aaagattacc
     1501 cgttctttat ggtagcgttc ttgcagatag tatgtttcgc tggtatcta
//
LOCUS       DACXMZ010000385         1528 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.386, whole genome shotgun sequence.
ACCESSION   DACXMZ010000385 DACXMZ010000000
VERSION     DACXMZ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.386"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            55..>1528
                     /gene="cls"
                     /locus_tag="JD016_19320"
     CDS             55..>1528
                     /gene="cls"
                     /locus_tag="JD016_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAU5001752.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSA"
BASE COUNT          579 a          187 c          240 g          522 t
ORIGIN      
        1 tgtgttatta tatataatta cttattttaa taaatatttg aaggagggaa ttttatgttt
       61 gatatatcat ttttcgaaat gtcaatatat gaaatagcag ttactacaat atatataatt
      121 aatttcatgg ttatactaaa ccttatcttt agagaaaaaa gaaacataaa tacaacttta
      181 acatggcttc ttatattagt attgattcct gctcttggat tcatacttta tatagctttt
      241 ggcagaaata tttcaaaaaa caatatgttt agattgaaag aaaaggatga taagataatc
      301 aaaagtaata tactagatac tcaggttaaa ctccaatcta catctgaaat tgattcagat
      361 atacatcaac ataaggatat gatttatgca ttggctaatt caaataatgc tcattatacc
      421 aataataacg atgtgtggat ttatgctgaa tctagtcaat tttttaacag tcttcttgag
      481 gaacttaaaa aagctaaaaa atatataaac atacaatttt acattttcaa agatgacaaa
      541 atcggaactg aaataataga cattcttata gacaaagcta aagaaggtgt tgaagtaaga
      601 cttttatttg atgcagttgg tggtagaaca ctaaaaaata gtacactttc cagacttaaa
      661 gaaagtggtg ttaaagttgg aagcttcttc ccatcattct tgaaaattgt aaactttaac
      721 ttaaactata gaaatcaccg aaaaattgtt gtcattgatg gtaaggtagg atttgtaggt
      781 ggatataatg ttggtgatga atatttaggt agaaatccaa aatttggtct atggagagat
      841 acacatacta agcttactgg tgattgcgta attgacctaa atatgagatt catccttgat
      901 tggagatata caactaaaga agacttagat ttagaaaagt actttataga agaagataca
      961 aattctaatc atccttctga aaatattggg attcagatag tatcaagtgg tccagatata
     1021 tcagaacttg atgagattaa atatggttac cttaaaatga ttcagaaagc tagaaagtat
     1081 atatacatac agagtcctta tttaatactt gactcaactt ttatagatac tttaaaaata
     1141 gcttgtttat ctggtgttga tgtgagagtt atgattcctt ctaaacctga ccatcccttt
     1201 gtatactggg catcatattc atatgctgga gaacttttaa agtttggagc taaaatctat
     1261 acttatggtc aaaatgcatt tttacatgct aagacaatag ttatagatga ctctatatgc
     1321 tctataggaa cagctaatat ggatatcaga agttttgagc ttaatttcga agttaatgca
     1381 tttatgtact cttcaaaaaa agcattggaa caaagaatta tatttgaaaa tgatatttta
     1441 aattctaagg aaataacatt agatatatat aattctaggt ctacttatat aaaaatcaag
     1501 gaatcaattt caagactatt atcagcag
//
LOCUS       DACXMZ010000386         1501 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.387, whole genome shotgun sequence.
ACCESSION   DACXMZ010000386 DACXMZ010000000
VERSION     DACXMZ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.387"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            36..509
                     /locus_tag="JD016_19325"
     CDS             36..509
                     /locus_tag="JD016_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAU5001753.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGIMTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(878..1435)
                     /locus_tag="JD016_19330"
     CDS             complement(878..1435)
                     /locus_tag="JD016_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAU5001754.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSFL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
BASE COUNT          544 a          206 c          163 g          588 t
ORIGIN      
        1 ttataattaa aataataata aaggtgttgt ttataatgtt caaagaatac ttaataagag
       61 aattaaaaga agatgatatt aaaaatgcat tagacttaat ctggaacgta tttgaagaat
      121 ttgaatctcc agattattgt gatgaaggaa ttatgacatt tcatcaattt atagaatata
      181 actctattat tgaaaaaatc aaagaaaaaa ctctcttctt ttggggttgt tttgactcta
      241 aaaaacttat tggagtctta gctattagaa atttaaatca tatatgtatg ttatttgtag
      301 ataaaaacta tcataaacat ggtgttgcaa aaaaattaat gactacaggc atacaatact
      361 gtatttgtaa taaatttaat ttaactgaaa taactgtaaa ttcatcacct tatgctgttg
      421 aagtttatca taaattagga tttaaagata ctgctgttga gcaaattgtt gacggtatac
      481 gttttacacc tatgaaattg ttaatataat aaattaaata aaaagcaaag tctcttaaat
      541 attttatagt tactttgctt ttaaaccaag atttaaaata atactatctt ttttgttttt
      601 taataatata ctttaaaatc cttactaata tgaactataa tttatttttt gatatccttt
      661 ttgattaaca tataaattaa ttcaattatt aaacataaac tatgttgttt ctctttatat
      721 ctcttctttg taaacaatat tgggcatttg aaaataaata ttcttaaaat atactgttct
      781 cctgttgatt ttcctttggt acaaatgata aaaatttaat ttacatgccc cataatgaag
      841 tgttcaataa ataagttaaa ataatttatt tacctcctta atcatatttt ttatgaatac
      901 ctaataacac agtatcaata gaatcgacta aaccttttat agcttcttct ttactcaaaa
      961 atgatttccc aagttgcaaa gtcgaataac catgaagtac actcattatt aagtttaata
     1021 cttcacctgt attctttgta tttagattac aagaaagaat aagtttttct aataaggatt
     1081 tatattcatc aaagatttct aatgtttcat tatttccatg ccaagtagcc cactgaattg
     1141 tttcataaac accaggatta gaaattataa aattaaagta tgctatactt atacacttta
     1201 tagctgtatc tccaaaattt ccaatagccg cctttatcat ttgactattc atctctctca
     1261 ttcctttatg agctacttca agtaataaat catctagact ttctatatga ttgtacaaag
     1321 atggtgtacg tattcctagc ttttctgcaa taacttttaa agaaactttg ttaagcccat
     1381 ctttattaac tatatctgat gtagcttgta taactatttc tctactaaca cgcatgattt
     1441 tccctctttc atttaaaatt aattacttta ttagtttaac taatctaatt agtttcgtca
     1501 a
//
LOCUS       DACXMZ010000387         1497 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.388, whole genome shotgun sequence.
ACCESSION   DACXMZ010000387 DACXMZ010000000
VERSION     DACXMZ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.388"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            68..1408
                     /locus_tag="JD016_19335"
     CDS             68..1408
                     /locus_tag="JD016_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAU5001755.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGMGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          580 a          113 c          245 g          559 t
ORIGIN      
        1 tcgtatataa ttaaattata cataaaatat aaaaaaataa tttagtaata cgttaggaga
       61 aagtattatg ataaagtcaa gcttattttg gaatattttc attgcgatat cctttatgat
      121 agaatggttt ctttgtaaga aaatgctaga ttatacaagt gagaaaagga tttctaacaa
      181 aaaaataaac ttttgtatgg taggtattat tgtttttgct gacataatat atttttttga
      241 tgtacctcca aatataagag taattatatc tatgataatt actattgcat tttataaaat
      301 ctcatatgat gtggggttta taaagtctgt attaatatcc ttaatttatt ggatgttact
      361 aatcgccaca gatgctttga gtatatcttt aacagtttgg cttaattcta taaatgacat
      421 gagtagtatg gggatgggag atatatacag aattgagtct ataatattgg gaaagatact
      481 tttgattgtg tctttgcttt tatatagagc cttaaagata aaacttaata tagaaataaa
      541 taaaaggaca ttattttata tagttattcc tatagtcgct aatatagcat ctttttttgt
      601 aatttttgaa tacatattta atccagggtc tggtagctta atacataatt tccaattcgt
      661 tatgatttct gttgtattgc ttatatcaag tatcacttta gttttaattg ctaaaaaatt
      721 acagcaatat aatactttag ttgtagaaaa caatatgatt aaaaatactt tgaagtatga
      781 aaatgagtat tatgaagata tagaaaagaa atatttaaaa actagattac taagtcatga
      841 tatgaaaaat cacatattgt gtataaatgc tatgattaaa aaaggtattg atgttacttc
      901 ttatttaaat aatttgcagg tagaaattca gagtaatgac ttgttattta atactggtaa
      961 ttttatatta gatgctatat taagtgaaaa gaaagaggtt tgtgataaga atgatataga
     1021 actaaaaata ggaataaatt ttagttgttg tgaagctgtt gaattagtgg atatatgtca
     1081 tatattttca aatattttag acaatgcaat tgaggcatgt aataaaattg atagtggaaa
     1141 tagagttatt tgtgttaaag gtgatgttat gcaaaatttt tatttaataa gagttgaaaa
     1201 tactaagatg aataagatta ttactaaaaa tggagatatt ttgacagaca aaaaaggttc
     1261 tcttcatgga cttggaataa aaagtgtaaa aaactctgta aataagtatg gtggtaatgt
     1321 tgtaattgaa tatgatgata aaaaatttgt agttaaaatt gctattccac ttagaaatgt
     1381 gaagaaagca gttaatcatg tgatttaaat gtatataaat ttgatgtgta gaaatttaat
     1441 atataagaat taatgtataa aagtgatgca tagaagttta atatataaga attaatg
//
LOCUS       DACXMZ010000388         1482 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.389, whole genome shotgun sequence.
ACCESSION   DACXMZ010000388 DACXMZ010000000
VERSION     DACXMZ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.389"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            61..1299
                     /locus_tag="JD016_19340"
     CDS             61..1299
                     /locus_tag="JD016_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001756.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT          629 a          137 c          254 g          462 t
ORIGIN      
        1 tttatataaa aatacttatc tagttactag caattattag tagaaatggg ggaaatagag
       61 atgaataagg gattttacaa attaaattca gtggtattga actttgatat taagaatgat
      121 gttaacaaga taagatttaa tcattatata atgagaaaag aaaacataca aagacttcaa
      181 ggaactctgc caaacggaca attttatatg acagtgagaa gagctgctac agatttacaa
      241 ctttctattt ctactataag taggcttgta aaagagtttt tggatttagg gataataaga
      301 ttagtgtcta agggtgtaaa aggtaatcgt tctgtatata gttacgtaag tatagagttt
      361 aatggaagtt tatttgatga tagcaaatat atggaacttg actgtgtatg taatgatgaa
      421 tttattgata atagtggcat agagtttgat tgtgatagca aagataattc ttttaatgga
      481 gatataaatg tagaaactgt gcattgcatt aatgagtatt tgtctgatga tgatgtagag
      541 cataattata acaatttcca taacaaagat actataaagg ataaagccat ttctaatatt
      601 agcactatca aaaaatatat tgatgaatct tttgatacaa ataaatcaga aaattctaac
      661 ttacaacagg agtcaataaa tttcaacttt aatgatacat gcaatgaaat agataaaaga
      721 acgaataata gtatagaaaa taatcaaaat aaaaggccta tgagattgag tgacatcagt
      781 cttttaagtg ataaaggtat gacaaataaa aagaattact ttaatcataa tcatggtata
      841 caaaaaggga ctaaatctgt aacaaagaaa aaagaatcct taaataaatt taataaaaaa
      901 aatatacagc aaagcattat aaatatgcta aataaaaaat caggtaagaa ttttaaaatc
      961 gattctccaa taagcataaa acttatcaat gaaaggctaa aggaaggcta tgtcttagaa
     1021 gatttttaca aagttattga ggtaaaagtg gctaagtgga aaaataccat aatggaaatg
     1081 tatataagac cagaaacttt gtttagctat aagtttgaat cctatgtaaa tgaaaacata
     1141 tctatgaaaa gtcaaagtaa tgttcaccca tgtaaagaag gcaatagtaa tgatagcagt
     1201 aattatatac attctgatta tacaacatat gatggcagtg tgcgtgagag aaaaaaatat
     1261 gagaatgtaa gatactggaa tcctatatgt gaaatttaat tagtttaaga atgtatttat
     1321 gataaataag atattaatag agaatagtga gctatagcaa atgatatatc aatagttggt
     1381 agtaagttat attaagcaag atattactat caggtagtga attgtattga acgatatatc
     1441 aataattggt agtaaattat attaagtaag atattactat ca
//
LOCUS       DACXMZ010000389         1474 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.390, whole genome shotgun sequence.
ACCESSION   DACXMZ010000389 DACXMZ010000000
VERSION     DACXMZ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.390"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(192..452)
                     /locus_tag="JD016_19345"
     CDS             complement(192..452)
                     /locus_tag="JD016_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001757.1"
                     /translation="MYLSIIFKLKMYKNTYIQLDIGIRGFQWWIRGESISGYLIVLLV
                     FVKLFIQIFTWNFIYFFMVFLSIFFYSWGIIGGFITIIKYIL"
     gene            942..1187
                     /locus_tag="JD016_19350"
     CDS             942..1187
                     /locus_tag="JD016_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HAU5001758.1"
                     /translation="MLIHTKNKTFDAICILEKIEKDLNIEELVRCHKSYIINLNYVEN
                     IKSNTAILDSQEEVPISRYRYKDIKEGFLKFIGDRIC"
     gene            1181..1453
                     /locus_tag="JD016_19355"
     CDS             1181..1453
                     /locus_tag="JD016_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001759.1"
                     /translation="MLNHINKILLILSLFTPIMILKSILTYDKDTDKTKKISFFTVLL
                     AYLITKIIILFNVDKTHNYNMKKNLYDIIYYLIFIIWELTIIIFIR"
BASE COUNT          692 a          151 c          173 g          458 t
ORIGIN      
        1 tgattaatta tatagttaag agatatattt aaaattagta ttgaagaatt aaatagattt
       61 gttaaagcta taaatgtaat taaaggaaat aagtgaatat tgtcatcaag ataaagttat
      121 gattaggata taacgaataa atgaagtggg taaatagtgc tgaaatttta atgaacaact
      181 agttggattc attataagat atatttaatt atagttataa atcccccaat tataccccaa
      241 ctgtagaaaa aaatacttag aaaaaccata aaaaaatata tgaaattcca tgtaaatatt
      301 tgtataaaca gctttacaaa aaccaacaaa acaatcaaat aaccacttat agactcccct
      361 cgtatccacc attgaaatcc acgaatacct atatccaatt ggatataggt atttttatac
      421 atttttaatt taaatattat tgataaatac atttaaaaag tgttaaccta aagtgaataa
      481 tctaaataaa aatacaatac taggagaggt catcattaca atagcagctt gtgatgatga
      541 aaaagaaata caaatcgtaa taaaaaatca agttgataat atattaaaaa ataaaaacaa
      601 agaatatcga attaaaaaaa tatagctcaa aagaagaacc attacaaagt taattgagag
      661 gatacactac tcttagatat aaaaatgcca gaactaaata gtatcgatat cgcaagaaac
      721 ataagaaaag tagataataa tatttataac atctctaata aattatgtac aggaaagata
      781 tgaagtaaga gcttattgat acttattaaa gccaataaat tttcaagaac tagaaaaaca
      841 catattaaca tgtatcaaaa aaattgaaaa aaataaaaat aagtctaaca catacaaaat
      901 ctactctagc aaaaaaatag atagaagttc aaaaaaagaa catgctaata catacaaaaa
      961 ataaaacttt tgatgctata tgtatactag aaaaaataga aaaagattta aatatagagg
     1021 aacttgtaag atgtcataaa agttatataa ttaacttaaa ctatgtagaa aatataaagt
     1081 caaatactgc aatattagac agtcaagaag aagtacctat tagtagatat agatataaag
     1141 atataaaaga ggggtttcta aaatttattg gtgatagaat atgttaaacc atataaataa
     1201 aatattatta atattatcat tgtttacacc aataatgata ctaaaaagta tattaactta
     1261 cgataaagat acagataaga ctaagaaaat atcttttttt actgtattat tagcttatct
     1321 tataactaaa atcattatat tatttaacgt tgataagacc cataattata atatgaagaa
     1381 aaatctatat gatataatct attatttgat atttattatt tgggaactta ctataattat
     1441 ttttataaga taggaattgt taaatatata gttt
//
LOCUS       DACXMZ010000390         1392 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.391, whole genome shotgun sequence.
ACCESSION   DACXMZ010000390 DACXMZ010000000
VERSION     DACXMZ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.391"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            535..924
                     /locus_tag="JD016_19360"
     CDS             535..924
                     /locus_tag="JD016_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAU5001760.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT          612 a           91 c          231 g          458 t
ORIGIN      
        1 atttagggag taggaagttt agtaataatt tgtagcaagt aagaaattta gaaagtagga
       61 agtttagtag taatttatag taagtaagaa atttagtaag tgagaagttt agttataatt
      121 tatagtaagt aagaaattta gtaagtgaga agttcagtaa taagttgtag taaataaaaa
      181 gtttaggaag taggaagttc agtaataagt tgtagtaaat aaaaaattta ggaagtagga
      241 tttactgaaa tacaaatatt taaaatcaaa gagtttaaaa gaaaggagag ctactggaca
      301 agtatccaaa atagatagct gagtacaaaa tatttcataa aattaagcaa tataatacaa
      361 gagaattttg acattattta tatatattta ccgtgtacaa atagctaaat ttaggattaa
      421 gattgaaaaa tatcttataa gtctgagctt atataaatcc gtataagatg tatttaatcg
      481 aaaaatttta ttatggatat tgcttgtcta tattctgagt agcaaggttt tatcatgact
      541 ataaaaaagt tgccacaatc agaattaaag ataatgaaat ttatatggaa atcagattcc
      601 aaagtaacat ctagagatat tgttttagga atggagcaaa aatatcaatg gaaacaaacg
      661 acaactttga ctcttttatc tagattagta gtaaaaagat ttctaaattc tcaaaagata
      721 gataaatata cgcattatga agttttaatt aaagaaaagg aatacatagg agttgagaca
      781 agagatttct ttagaaatat tcatgattct tctataaaaa gtttattgtt gtcattacat
      841 gaaaatatta atttgagcaa agatgatatt ttacttattg aagagtggat aaaaaatcta
      901 aaagaagagg agaaagacgt ataagcaaac gtatttctta tagcatcaat ataaagtgta
      961 tgtaagtgta tttatgtaat atattagtct acttaagtaa tatgacaaat gattataagt
     1021 ggattatgaa actataatgt gaatctaaat aaattaaata taaattaata gtggatattc
     1081 tatttaatta aatttaaaaa gctaccttta cttataagta taattaaggt agctttttaa
     1141 atagaggtag tgtaattaaa gagaattttt aaatataaat tgtgaagctt taatatagat
     1201 gataatatat attttgcaaa tatgacacag tgttttcgta tttaagatga taaaataaat
     1261 aggaattaat gatgatattg aaaaaataaa aataaaggat taatataaga tattaaagag
     1321 atagagataa aagtgttaac agaagatatt gaaaaaataa aagattgata taagatactg
     1381 aagaagtaga aa
//
LOCUS       DACXMZ010000391         1381 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.392, whole genome shotgun sequence.
ACCESSION   DACXMZ010000391 DACXMZ010000000
VERSION     DACXMZ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.392"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..332)
                     /locus_tag="JD016_19365"
     CDS             complement(<1..332)
                     /locus_tag="JD016_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAU5001761.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKAIEWELKAIDSD
                     RDRQLRKDSTIRVPVLNKKGKATGQYTSETDFNTYTLKLCVETVVFPDLHDAELQNSY
                     GVMGAEEL"
     gene            complement(337..594)
                     /locus_tag="JD016_19370"
     CDS             complement(337..594)
                     /locus_tag="JD016_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001762.1"
                     /translation="MIKIKDKYEIKDSISFDYSNKRPLEERVSEMYKKAGKYLIDISD
                     KLATDTIDGSSLKPIIIKFEINEAGVATIEKQTKYLVMEVE"
     gene            complement(647..1117)
                     /locus_tag="JD016_19375"
     CDS             complement(647..1117)
                     /locus_tag="JD016_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001763.1"
                     /translation="MAQQIKARDTISASKAECFVTIKGKRYNFMQAINLEAKMEKNKS
                     EIPILGSTTKGNKSTGSKYSGNATFYYNTSIFRELLYEYKETGEDIYFDIQITNEDPT
                     SSVGRQTIILEDCNMDSGIIAKFDADGEYLDEDMDFTFENWKLVEKFNIANGME"
     gene            complement(1139..>1381)
                     /locus_tag="JD016_19380"
     CDS             complement(1139..>1381)
                     /locus_tag="JD016_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAU5001764.1"
                     /translation="ATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNMRAIEDFKA
                     DDVSVEPGSDKKTVVVSDAVKVISAMSKLYMTVSVS"
BASE COUNT          398 a          285 c          138 g          560 t
ORIGIN      
        1 aatagctcct ctgcccccat tacaccataa ctattttgaa gttctgcatc atgtaaatct
       61 ggaaatacta cagtttctac acacagtttc aaagtataag tattaaaatc tgtttcacta
      121 gtgtattgcc ctgttgcttt ccctttttta tttagtacag gtactcttat agttgaatct
      181 tttcttaatt gtctatctct atctgaatct attgctttaa gttcccattc gattgctttt
      241 ccatcttctc ctataaacct ttcacttgcc acatactttc tattctctac ttttattgca
      301 ttttgactta aaaaagcgtt taaatctccc atattcttat tctacctcca tcaccaaata
      361 ttttgtttgt ttttctattg ttgcaacacc agcttcattt atttcaaatt ttatgattat
      421 tggctttaat gacgaaccat caattgtatc tgttgctaac ttatctgaaa tatctataag
      481 atattttcct gcctttttat acatctcgct gacacgttct tccaaaggtc ttttattgct
      541 ataatcaaaa gaaattgaat cttttatctc atatttatcc ttaattttaa tcatctccta
      601 ttcttataaa taaaaaatac acatacataa atcataaatg tgtattttac tccataccat
      661 ttgctatatt aaatttctca actaatttcc aattctcaaa agtaaaatcc atatcttcat
      721 ctaaatactc cccatcagca tcaaatttag caattatgcc tgagtccata ttgcaatctt
      781 ccagtattat agtttgacga cccactgaac ttgttgggtc ttcattggta atttgtatat
      841 cgaagtaaat atcctcacca gtttctttat actcatacaa caattctcta aatatagagg
      901 tattataata aaatgttgca tttcctgaat atttacttcc tgttgattta tttccttttg
      961 tagtactacc taatataggt atctcactct tattcttttc cattttagct tctaagttaa
     1021 tagcttgcat aaaattatat cttttacctt ttatagttac aaaacactct gccttagatg
     1081 cacttattgt atctcttgct tttatttgtt gtgccattat tattctcctt tcttattgtt
     1141 aactaactga aacagtcata taaagcttac tcatagcact aataactttt acagcatcac
     1201 ttactacaac agtcttcttg tcgcttccag gttctacaga aacatcatca gctttgaaat
     1261 cttctattgc tctcatattt tgcagttgtt catgatgctt aactacatca ttccaaaacg
     1321 agatacgacc agatttatca tttggtactt cacccaagta ctttgtatta aataaagtcg
     1381 c
//
LOCUS       DACXMZ010000392         1306 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.393, whole genome shotgun sequence.
ACCESSION   DACXMZ010000392 DACXMZ010000000
VERSION     DACXMZ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.393"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..486
                     /locus_tag="JD016_19385"
     CDS             <1..486
                     /locus_tag="JD016_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAU5001765.1"
                     /translation="YFDNMRLSQDFGFNHANVVLRIGFKDGELYICNEIYVHEMDTSE
                     IIKIANSRGLEKTLFMYCDSAEPDRIKMWKSAGYKAKGVKKGPGSVKAQIDYLKQLRI
                     HVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEFMDDAMAALRYSIDNKLKNNGISFL
                     K"
     gene            492..>1306
                     /locus_tag="JD016_19390"
     CDS             492..>1306
                     /locus_tag="JD016_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAU5001766.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFVERGNSFVQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTL"
BASE COUNT          537 a          135 c          243 g          391 t
ORIGIN      
        1 tattttgaca atatgaggtt atcacaagac tttggattta accatgcaaa tgtagtactt
       61 agaattggct ttaaggatgg agaattatat atatgtaatg aaatatatgt acatgaaatg
      121 gatacttcag aaatcataaa gattgcaaat agtagaggtt tagaaaagac tctatttatg
      181 tactgtgata gtgctgaacc agatagaatt aagatgtgga agagtgcagg atataaagct
      241 aaaggagtta aaaaaggacc aggaagtgtt aaagctcaaa tagattattt gaaacaatta
      301 agaatacatg tacatcctag ctgcactaat accataaaag aaatacaaca atggaaatgg
      361 aaacaagatg aaagaactgg attatatctt gatgaaccag ttgagtttat ggatgatgca
      421 atggctgcgc ttagatattc tatagataat aagcttaaaa ataatggaat aagcttctta
      481 aagtaaagga ggtgttaaat atttatataa gtgaaacaga tttaataaaa gttcagttaa
      541 aaaaagagag cacctttaac ctagtaaaag tcatagaaca ctacatctta aagcataggc
      601 cagaaaaata taaacaagga gaagaatact attatggtaa tactgatgta aacaataaga
      661 gaagatatta tctcttagat ggagctaagg ttgatgattt tactaaggtt aataataaag
      721 caattaacaa ctaccataag cttttagttg accaaaaagt gggctatagt gttggaaatc
      781 caatcgtatt taatgcagat gatgataatc tcactaagct tttaaatgac ttactaggag
      841 aagagtttga cgatacaata acagaactat atctcaatgc tagtaataaa ggggttgaat
      901 ggttacatcc atatattaat agaaaaggtg agtttaaata tgtaataatt ccagctgaag
      961 aagcaattcc tatttgggat agtaaaagac agagggaatt agttgcattt attaggtttt
     1021 attatattga agatatagat ggaaataaaa taaaaagagt tgagtactac acagaaaatg
     1081 atgtaactta ctttgttgaa agaggtaata gttttgttca agaattttta tatgatgaat
     1141 atggaaaaat gactgatata caagaaggtc attttagaat aaataacaaa gaacagggtt
     1201 ggggtaaagt tccatttata ccttttaaaa ataatgaaaa gtgtgtctca gatttaactt
     1261 tctataaatc attaatagat atatatgaca ataatatttc tacact
//
LOCUS       DACXMZ010000393         1302 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.394, whole genome shotgun sequence.
ACCESSION   DACXMZ010000393 DACXMZ010000000
VERSION     DACXMZ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.394"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            191..1288
                     /gene="ychF"
                     /locus_tag="JD016_19395"
     CDS             191..1288
                     /gene="ychF"
                     /locus_tag="JD016_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAU5001767.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT          505 a          141 c          239 g          417 t
ORIGIN      
        1 atatttttat ttaaattaaa tacaaatatt atttaaatca atatgcttta aagtctagtt
       61 tacaagcttg atatatatga tgtataatca atatgtatgt ctataattaa aacaattttg
      121 caatcctata gattgattgt ttaaagttat ataaagaata taaaaaagaa taagagagag
      181 gacgattaat atgaaattag gtatagttgg tttaccaaat gtaggtaaaa gcacactatt
      241 taacgcaata actcaagcag gtgcagaatc tgcaaattac cctttttgta caatagaccc
      301 aaatgtggga gttgtatctg ttccagatga aagattaaac aaactacaag aattatataa
      361 ttctgaaaaa atagttccta ctgctataga attttgtgat atagcaggtt tagttagagg
      421 agcttctaaa ggagaaggtt taggaaataa attcttatca catataagag aagttgatgc
      481 aatagttcat gttgttagat gttttgagga tgaaaatgtt gtacatgtag atggttctgt
      541 agacccactt cgtgatatag aaactataaa cttagaatta attttctcag atatagagat
      601 tcttgaaaga agaattaata aaactcaaaa agctgctaag gctgataaaa ctttaggttc
      661 tgaacttgat ttattaaaat caataatgtc tactttagaa gaaagtaagt gtgtaagaac
      721 tatggaattt acagaagatg aacaaacatt cgtaaattca ttggatttac ttacatctaa
      781 gcctgtaata tatgcttcaa atgtatcaga agatgattta gctgataatg gagaaaataa
      841 taaatatgtt caacaagtta aagcatttgc agaaacagaa gatgccgaag ttgttgtagt
      901 atgtgcacaa atagaagcag aaatttctga acttgattct gctgaagaaa agaaagagtt
      961 tttagaaaca ttaggtcttg aacaatcagg gcttgataag cttataaaat cttcttatgc
     1021 tttacttggt ttaatttcat tcttaactgc tggtccaaaa gaagttagag catggactat
     1081 aaaagttgga agtaaagctc ctcaggcagg aggaaaaata cattctgata tagaaagagg
     1141 atttatcaga gcagaaacaa tagcctttaa tgatttggtt gagcatggta ctatggctgc
     1201 tgctaaagaa aaaggattgg ttaaattaga aggtaaagaa tacatagtta aagatggaga
     1261 tgtaatatta tttagattta atgtttaaaa tgattaatcg ta
//
LOCUS       DACXMZ010000394         1302 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.395, whole genome shotgun sequence.
ACCESSION   DACXMZ010000394 DACXMZ010000000
VERSION     DACXMZ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.395"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(201..515)
                     /locus_tag="JD016_19400"
     CDS             complement(201..515)
                     /locus_tag="JD016_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001768.1"
                     /translation="MMKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSI
                     ISRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNK
                     TK"
     gene            complement(568..1161)
                     /locus_tag="JD016_19405"
     CDS             complement(568..1161)
                     /locus_tag="JD016_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAU5001769.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT          458 a          194 c          164 g          486 t
ORIGIN      
        1 ctaatttaag aaatatagaa aattaattta aagaaataca atacattaat ttttaagtaa
       61 cattaaatta atgtaagtaa aataatgttc atatgaagat ttaaaggtgc attttctgaa
      121 gctaagtata tgttgtagag tgttatgtct ctctatccgg tgaagtttaa tagctcaaga
      181 aaatgcacta tttatgatga ttattttgtt ttatttccaa ttttggaaat aactattcta
      241 attaaatcta aaattatacc ataaataaga aaagaaagta tagatgctat gtaccaataa
      301 atcgaaattg accctgtaat tgttctacat gggatttgca tacctgaatc cataaaaaca
      361 aaaaaactat caactctgga aataatagac aaaataggat ttaaacctat tagaacaata
      421 ctttttgagt cttgaccaat ttgatgcata taaatctcaa ataatgcaat taaaaaaaac
      481 caaaatgtaa aagatttaat aatttttttc atcaaaatac ctcacataat ataaattctg
      541 tttgcactat ttgcttacta caactattta tctccattca gtcacatcac ttaattgtaa
      601 tctaggtctt cttgatggtt cttcatcagg ataacccaaa gatattgctg ccactaattg
      661 actatctgta tttaaccatt cacataattc acgatatgca aagtatacat cacaaatcca
      721 aagactacct agacctaatt ccacagctgt aagcgacata ttctgaattg cagctccaat
      781 tgattgcatg tttgccattt catagaatcg ttcttcaggg gatagctttt ctaaaggtga
      841 tttacctaat atatttaaaa taaaaattgt tactggagct tgtttcatga tttcaactgt
      901 gtaatttgca ccagcgatat gttgacgact tcctggaaga agcccattat cattaatttc
      961 attttgaata cctttgctca tggctttaag catagattct ttttcctttt cagtgatgac
     1021 aacaaaacgc catggttgcc tgtttttaga tgaaggtgca tttattccag cttcaattat
     1081 tttctctata gtttcatgtg aaatgctttg atttttatat tttctgatac ttcgtctttt
     1141 tgatatagaa tcacttatca ttttaagtaa cctccgttta ttttttggaa aaatctattt
     1201 aatttttatt ttctatttta attattttag cattaatcag aaaaaaagtc attgatttta
     1261 gtatacaatt attaatgatt atattaagaa ttgtatcaaa aa
//
LOCUS       DACXMZ010000395         1249 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.396, whole genome shotgun sequence.
ACCESSION   DACXMZ010000395 DACXMZ010000000
VERSION     DACXMZ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.396"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            23..295
                     /locus_tag="JD016_19410"
     CDS             23..295
                     /locus_tag="JD016_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001770.1"
                     /translation="MVDMIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVN
                     YILENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(732..1109)
                     /locus_tag="JD016_19415"
     CDS             complement(732..1109)
                     /locus_tag="JD016_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAU5001771.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT          513 a          168 c          108 g          460 t
ORIGIN      
        1 gatgatataa ttttcttaag aggtggttga tatgataaaa aatacaaaac aaattacagt
       61 agaagttaat gaaattttat tacaagaaat gtctaagata actaatgacg aaaataatat
      121 tttgacagat ttgataaatg aatcaattgt aaattatatt cttgaaaaca aaaaacataa
      181 tattgaaaat aaagatttag aagaagtgat taaattttac aaaaaagaaa atactgaaaa
      241 acctaaaagt gcagcaagag ctatagctga agcaaaaact catactctgt attaatcaaa
      301 taaaaaagtt cctaaaaact aggaactttt ttatattaat aattattaat atatctcaat
      361 atttatctca aatctatcaa tataagcatg tttaatacaa tctattgtat acttatctta
      421 ccctagaaat aatcaaaaca aaaataaaat tatgcccttt atatctaaaa caattaattt
      481 acatgattaa tatacaaaaa ttctgacttt taaattcgta tacaatatca aactaaaata
      541 atattaatga caaacccaat ataaattaaa ttcaaacagg tttgatttta catcaaactt
      601 attgtattaa tattaaataa caagacaaaa atatttataa aaatcacaaa ttttacataa
      661 aactttatat ttgattcatt taactaatta ttgtaaaaac tatttatttt atgataatta
      721 actacaccaa attattcatc ttttttaata tctttaaccc atttttcaac agagttcaat
      781 tcttcttcat ttaccccctt tttgaacaag gatttcataa aactctcaat agaacttcca
      841 tgcatattat ctaaaaaagt ttttgtttca aaatgtaaat actcttcttc tccaataaga
      901 actgtataat gtgtatgtct atcaattttt cttgcactta agaattgctt atttactaat
      961 cttgataaaa gtgttagtgt tgtagtttgc ttccaattat atagtttttc catagcttca
     1021 atgacatctt ttgatgctac tgtaaaatct gaactccaaa taaatttcat gacttttaat
     1081 tctgctggtg gtatcttagt aatagtcata tgattttaca ctcctctcaa acttctacat
     1141 aattaaatac tatattaata gacttttcga gtcaaaggtt ttatttaaaa tatctcatcc
     1201 aaatttatta atacaaaaaa tttatatttt tattcaaaat taaacacac
//
LOCUS       DACXMZ010000396         1244 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.397, whole genome shotgun sequence.
ACCESSION   DACXMZ010000396 DACXMZ010000000
VERSION     DACXMZ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.397"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(119..385)
                     /locus_tag="JD016_19420"
     CDS             complement(119..385)
                     /locus_tag="JD016_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HAU5001772.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(427..1236)
                     /locus_tag="JD016_19425"
     CDS             complement(427..1236)
                     /locus_tag="JD016_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5001773.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT          429 a          179 c          148 g          488 t
ORIGIN      
        1 tattctatta atttaattaa ataaccttta aatttaatta taaaaactgt tgcagaaatt
       61 tctacaacag tttaaaaaat ttaccttaca aaaacttatt ttataacaag agagtatatt
      121 ataaggctag tacagcccat tttttgcatt gagaaattct tttgccatca tcaccttgtt
      181 ctttgtcata tgcaaattga ttaagcattt cacatggaaa tgtatcacat agaccacaat
      241 gtgtaaggtt tttatcttca caacaagatt ttacaggaca ttgttcaccc caaaatggtt
      301 tctcaatctc tgtacaacct ttacaattga ctttttcctt gtatccacat tcataacata
      361 aaattccaca tcttgattct atcattttaa gtcactcctt aaatataaat tttatatgat
      421 tgtatatcaa aatttatact tttcattgta aaaatcggac aaatttttta tataggttgt
      481 tggattaaca ccacaaatag atttaaagtc atttataaaa tgagattggt cgtaataacc
      541 aatatcttgt gctaaagttg caaatggtat tttattattt tgtatttctt gtaatacaag
      601 gtttatacga agtaatctaa ggtatgattt aacactcata ccaagagaat tattaaaaat
      661 tctatttaaa tgtcgttcac tataacaact aatttgtgaa atgtttttaa cagacataat
      721 agagttttgt tttctagcat tttcaagaat tggtatagtc atatctacta tatttgattt
      781 caataagtat gataagaata acatatctaa ttgttctact aattccttta tagtacttgt
      841 tctttcaaaa atggaattaa cctctaagct aaataatgtg ttaaaatctt caagtgaaat
      901 ttttaaatct gtagattctc tttgagaaag acctgtcaaa taatatactc cacctgggcg
      961 aaactcaacg aatactctaa aaagtacatt ttctatatca ttttttacaa tagtagtttt
     1021 tgttgtggct ccccagaatg cgctttctat accttttttg tcaaatttaa atatcataca
     1081 tccactagca tcaggtatta atgtaagatt ttctttaata cttgtatctg gaatagatat
     1141 tgtatagtgt gctatatact gtcttaacaa tttatgtggc aaaatatatg tatactgttt
     1201 gcaggtattt aaaatgttat tgattatttt attcataagt atat
//
LOCUS       DACXMZ010000397         1242 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.398, whole genome shotgun sequence.
ACCESSION   DACXMZ010000397 DACXMZ010000000
VERSION     DACXMZ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.398"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            157..867
                     /locus_tag="JD016_19430"
     CDS             157..867
                     /locus_tag="JD016_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5001774.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNTITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          497 a          101 c          195 g          449 t
ORIGIN      
        1 atcgtaattt aagtttaaat attaaagaga atttctataa gtagtttaat cgacaattta
       61 aatttataaa gaaatttaaa tttgaaagct agtgagtaca gtttggaaat tgaaataaag
      121 taaactaaat gatattttag atttggaggt gatattatgt tcagggtagt tatatgtgat
      181 gacgaaaaaa tacaaagaag catactaaaa gagtttacag aaaaactttt gtctgaaaga
      241 tgtctaaatt atcaaatact ggaattttcc tgtggagaag atttaatatc caaataccca
      301 gaaaaaattg atattatatt tttagatatc caaatgaaag atatcaatgg aattgaggtg
      361 gctaaaaaaa taagaaagtt tgatgaaaaa gtagaaatta tatttacaac ggctttttca
      421 gagtatgccc cccaaggtta tgaagttcgt gcttatagat atttagtaaa gcctatagag
      481 tatagtaact ttgcaatggg tgtaaatctt tgtattgata accttcaaag aaagaaggag
      541 cgttatatag ttttaaatag taaaaaagga tttaatagaa tactgataga ttctatttta
      601 tatgttgaaa ctgtaaagag agatttagtt gtacatacaa taggtagaga ttataaagct
      661 aatatgagca tgaaacaagc agaaaaatta ctgtgtgaaa atggcttttt tagatgtcat
      721 acaagtttta ttgttaattt aagaagaata gaagatctta gggataatac gattactatt
      781 aatgaacaat ttatacctat aagtaagtat agacttaaag atttaaaagt agcattaaca
      841 agcttgttat gtgatataat gtattaattt taaaaaggta attgaaaata tcgtaaaaaa
      901 gatttaatgt catcacaggg ctattgattg aaactgtctt aatatataaa atgttattta
      961 tattaagaca gttttattat gcaaaatagc agtttataga tttttaatat ggtttaatat
     1021 gtatatgtag agtttccatt ataatagcat gattattaat tagtagcttg attatcaata
     1081 tactattatt gatttttata tgcattatat gtatttttag aatatgatta cctttttaaa
     1141 ataatgacta tttataaaac tctatagata tttgtttcat ttagctacgc atactttaat
     1201 taattatatt aatatttaat ggagtacaat ataaatatta aa
//
LOCUS       DACXMZ010000398         1234 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.399, whole genome shotgun sequence.
ACCESSION   DACXMZ010000398 DACXMZ010000000
VERSION     DACXMZ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.399"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            176..>1234
                     /locus_tag="JD016_19435"
     CDS             176..>1234
                     /locus_tag="JD016_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HAU5001775.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDI"
BASE COUNT          486 a          176 c          218 g          354 t
ORIGIN      
        1 attcagactt atgaaaaggt agaaaatgaa tgttttcata aaatatataa aataaattag
       61 atataaaaaa taaattttat gttgacatag ttaattcagc ttgatattat agttattaaa
      121 aataaggaca gtaaaaacgc tatccatatt acaaactaaa aaggggggaa ttataatgag
      181 taagaaatta tttattcctg gtcctattga tgttaaagaa gaagtcttgc aaaagatggc
      241 tacaccaatg ataggacata gaggaaaaga tgcatctatg ctccaaaaga gtataagtga
      301 aaagatgcaa aaactattct atacaaacaa cacgatactt ttatccacgt catccggaac
      361 tggtttaatg gaaggctcca ttcgttcctg tacatccaaa aaggcagcag tattttcctg
      421 tggatccttt ggtgatagat ggtacaaaat ggcagtggct aataatgtcc cagcggatat
      481 atttaaagtt gaattaggag aggcaacaac gccagaaatg gttgataagg tgctttctac
      541 cggagaatac gatttaataa ctgtaactca caatgaaact tcaactggaa taagaaatcc
      601 aatagaagaa attggagaag ttgttaaaaa gtatgaagat gtaatctact gtgtagatac
      661 tgtaagttca gccggtggaa taaaagtcga ggttgataag attggaatag atatttgtat
      721 aacatcagta caaaaagcac tgggattacc accaggaatg tctatttgca cattttctca
      781 aaaagcaatc gatagagcta agcaagtacc ttttagagga gtatatcttg atttgttagc
      841 aatgtatgaa tacttaatca agaaaaacta ccaataccct tcaacaccat ctctttctca
      901 catgttcgct ttagatttcc aattagataa tatattagat gaaggcctag ataacagatt
      961 caacagacat gaagacatgg caaacttagt tagaaactgg gctaaaaaac acttccaaat
     1021 ttttaccaat gaaaatcact tatccaatac acttactgtt atagaaaata ctcaaggtat
     1081 tagtgtatca aatttaaatt caaaattaca agaacgagga ttccaaatag caaatggcta
     1141 tggagattta aaagaaaaaa cttttagaat ttcacatatg ggtgactata ctgtcgaaga
     1201 tgtacaagaa ttattagata atattgatga tata
//
LOCUS       DACXMZ010000399         1216 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.400, whole genome shotgun sequence.
ACCESSION   DACXMZ010000399 DACXMZ010000000
VERSION     DACXMZ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.400"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            255..1211
                     /locus_tag="JD016_19440"
     CDS             255..1211
                     /locus_tag="JD016_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAU5001776.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELREGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFIKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
BASE COUNT          504 a           99 c          170 g          443 t
ORIGIN      
        1 gcacatgcat tttatttttt agctttttta gtatcaataa ttttttagta tcaataatat
       61 gaaatatgtt ttaaaataag tatatagtgt aatctgtaat aaaaactttg gttaaataat
      121 aaatgtattt aacacttacc ttataataaa tttaatataa atattaaata ataattatta
      181 aaatagtgta aaataattac agtaaggatt ataaaaaatt tttagaatgt aaattgaaat
      241 ttaggagtag atgtatgtat atactgaatt taaatacaag agagactatt gaagatttta
      301 gagataaatt ttatgtagca gaaaattctt atcttatatt aagtgctcca aaaaatttaa
      361 aattgcttaa ggaaactttg gatatagatg aaataacttt taatgattgt cttaaatttg
      421 atgaaataac caaacttgat ttatttgata attacgattt tttaagccta aatacatttg
      481 aactaagaga aggagaagct gttatagaag aggtaaatat gtatttatca gataatttta
      541 tattggttgt agtgaatgaa gaacactttt tatttgaatt tataaaaaat attattttga
      601 agaactctca acttgaaaaa aatccagtta ttaatctatt taagataaac tacttgattc
      661 ttagagaagt tataaaaaat ggttttgagt ctttggaaaa agttgaagag ttaattcttc
      721 aaatagaaga tgaaatgatg gacaacatta ataagaatca tgtatctaga ataagtgatg
      781 taagaggatt aacaagaatt atagttaaga atactagacc tcttttgtat attggggata
      841 gaatagttaa ggaaaatata agatatttaa aatattcaaa tgtaaaaaaa tataatttag
      901 aaaattttca aggcatagat tttggaattg acaaattata caatttcgca ttgtcaacac
      961 gagagttagc agataaatta ttagatattt attcttccag agtaggtgag aaaacaaata
     1021 acttaattac aaaattaacc ttgttaacag ccatatcagc accacttaca attataactg
     1081 gtatatatgg aatgaacttt agatatatgc cagaattaaa ctggatttat ggatatccag
     1141 caactttatt ttttatgcta tgtataattt ttgttggaat tataatattt aaaataaaaa
     1201 aattattgta aattac
//
LOCUS       DACXMZ010000400         1206 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.401, whole genome shotgun sequence.
ACCESSION   DACXMZ010000400 DACXMZ010000000
VERSION     DACXMZ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.401"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            19..276
                     /locus_tag="JD016_19445"
     CDS             19..276
                     /locus_tag="JD016_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001777.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(386..1066)
                     /locus_tag="JD016_19450"
     CDS             complement(386..1066)
                     /locus_tag="JD016_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAU5001778.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYLIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          496 a          148 c          132 g          430 t
ORIGIN      
        1 gtaaatggag gaattgatat gataataatt agaagtcaag ataaaactaa tttaatgcag
       61 ataaaccaaa ttaaaataga tggtagtcaa gtttatgcag tatttgaaag taaaattgat
      121 actgtaaaaa taggagatta tgaaaataat acaagagcta tacaagtgtt ggacaatata
      181 caaaacttca ttgaaaatgg aacaaaatat gattatataa catcaaataa agtcagatac
      241 aatgttaata aaatatttca gatgccagct aaatagttag tgtgcatttt aatttaataa
      301 ctaaagccca ctttcttttg tagtatgaaa gagggcttta catatttgta aatatcgaag
      361 tacacttgtt ttatcaataa attatttaat aaggtataat ggaccaatta aacaataaaa
      421 attctcctaa aacagcaagt attaaatata taatagcttc atttttgtaa agagaggtaa
      481 ttttttcgct tatgtcatca tcaattgtaa tagcaatttc atcttgaata atatttttat
      541 atatagatgg gaaaatatct tttttcttta taagattaaa taaattattt acacctaccc
      601 atatcaaata tcctaaaaca gcacctaatg tgttcatcat taaatcatca atatctgtag
      661 ctcttaagtt aaacaattga caaagttcaa ttgctaatga aaaacctaat ccagttaatg
      721 aaacattgat aatatttcta aaatttttcc aaattaaagg tagtaaaaat cctaaaggca
      781 taaacataat tatatttaaa atatatgtta agatgccaac tgagtcaaaa ggtattagat
      841 ttatttcatc aattctaaaa agcccactat accttcctat atcccatatg ctaccaattc
      901 cagctttact aagtgctaga tagatatata ataaaaaaat atatacccat actaaatgta
      961 tcattacatt cttttcaact ttcaaatttt ttcgttttat aacgactatt tggtatatta
     1021 agcatggtaa tataacgcaa ataaatgaat aaaatgtcca tatcatttta ttttgtcccc
     1081 tttcaaattt atgtttttag ttttataagt actatcttta tattaaactg taagttttaa
     1141 aaatctttta ataaaatctt aaaaatttat taatttataa aaatatatta ttaagattaa
     1201 aaaata
//
LOCUS       DACXMZ010000401         1189 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.402, whole genome shotgun sequence.
ACCESSION   DACXMZ010000401 DACXMZ010000000
VERSION     DACXMZ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.402"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            65..1105
                     /locus_tag="JD016_19455"
     CDS             65..1105
                     /locus_tag="JD016_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAU5001779.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT          479 a          124 c          235 g          351 t
ORIGIN      
        1 gatataatta aatagataat gtgaatgatt attatataga atacttattt ggagttgata
       61 ttttatggac aaacacagtg attcagtaat gtatggtgga atggaagtat ataatatttg
      121 gggaacaaca acagtagact ccataattgg agagtctgat gtaatgcgtg ctcttaagaa
      181 tagaatttgt aaaattggaa atagtgattc tactgtagct ataactggag aaagtggcac
      241 tggaaaagaa ttaatagcaa gagccattca tctcgcagga aacagaggga ataaaacatt
      301 cgttgctata aactgtgctg ctataccaga acctttacta gaaagtgaac tttttggata
      361 tacaaaagga gcttttagtg gagcagattc aaaaggtaaa attggtaagt ttgaattggc
      421 caatggaggg gttgtatttt tagatgagat aggagatatg ccaattcaac tacaatcaaa
      481 gttattgagg gtattacagg aaaagaagtt tacgagaata ggttcaaatg aactaattga
      541 tatagatgtt agaatcatat cagctactaa tagagattta tttgagttgg ttaaggaaaa
      601 taaatttaga gaagacttat actatagatt gaatgtaata cctttagaag tgcctccact
      661 tagagaaaga ggagacgata tacaaatatt aataaacaat tttatagaca aatattcgag
      721 aaagatgaag aaaaacgtgt accatataga gccagatgtg gaacaaatgt tgcttaagta
      781 tccttggcca ggaaatatta gagagttgga aaatactata gaactggcat taaaccttct
      841 tgaggaagat ggaataatac ataaaggaat aataaataga aagatacttg aatattttaa
      901 gttaaataat ataaatataa agttacttga ggaaagtgac tatgagataa gtatggaaca
      961 agacatactt acacttgaag agtttgaaag agcctatata aagaaagtgt taaagtttta
     1021 cggaaatgat actaagtcta aaaaactggc tgccaaaaaa cttggaatat cactagctac
     1081 attgtacaga aaaatagatg tataataatt aggcttttta ttctcaattc taaaatttga
     1141 aattataaaa ttgataaaaa acacttggaa aactattttc taagtgttt
//
LOCUS       DACXMZ010000402         1188 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.403, whole genome shotgun sequence.
ACCESSION   DACXMZ010000402 DACXMZ010000000
VERSION     DACXMZ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.403"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            376..>1188
                     /gene="bclA1"
                     /locus_tag="JD016_19460"
     CDS             376..>1188
                     /gene="bclA1"
                     /locus_tag="JD016_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HAU5001780.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVT"
BASE COUNT          488 a          139 c          203 g          358 t
ORIGIN      
        1 acaaaaataa agttttttat aatttttact tataaaaaaa tattgttatg gtaatatatt
       61 ctatttacaa ttttataact ttggtttaca ataatataga caataaatcc taaaaaacat
      121 aataagaaaa aagtgcttaa tattttagaa agactggttt agtttacacc agctaagcca
      181 gtcaaggaat aatttaaagt gtaatatata ataatgaaaa agaacatgct attagaaatt
      241 tctaatgaca tgttcttttt ttgtagaaat cagcattatt atataaaaat ttaatactat
      301 aaattttaat tatattgtaa ctaagcatgt attttaaaca tagtatgata tagacccaaa
      361 atggagggat tataaatgag aaatattata ctttatttaa atgatgatac ttttatatct
      421 aaaaaatatc cagataaaaa ctttagtaat ttagattatt gcttaatagg aagtaaatgt
      481 tcaaatagtt ttgtaaaaga aaagttgatt acttttttta aagtgagaat accagatata
      541 ttaaaagaca aaagtatatt aaaagcagag ttatttattc atattgattc aaataagaat
      601 catattttta aagaaaaagt agatattgaa attaaaagaa taagtgaata ttataattta
      661 cgaactataa catggaatga tagagtgtct atggaaaata tcaggggata tttaccaatt
      721 gggataagtg atacatccaa ctatatttgt ttaaatatta cgggaactat aaaagcatgg
      781 gcaatgaata aatatcctaa ttatgggtta gctttatctt taaattaccc ttatcagatt
      841 cttgaattta catctagtag aggttgtaac aaaccgtata tacttgtaac atttgaagat
      901 agaattatag ataattgtta tcctaaatgt gagtgtcttc caattagaat tacaggtcca
      961 atgggaccaa gaggagcgac aggaagtaca ggcccaacag gagtaacagg cccaaccgga
     1021 agtacaggag cgacaggaag cataggacca acaggcccaa ccggaaatac aggagcaaca
     1081 ggaagtatag ggccaacggg agtaacaggc ccaaccggaa gtacaggagc gacaggaagt
     1141 ataggaccaa caggagtaac aggtccgaca ggaaatacgg gagtgaca
//
LOCUS       DACXMZ010000403         1175 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.404, whole genome shotgun sequence.
ACCESSION   DACXMZ010000403 DACXMZ010000000
VERSION     DACXMZ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.404"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..146
                     /locus_tag="JD016_19465"
     CDS             <1..146
                     /locus_tag="JD016_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HAU5001781.1"
                     /translation="IEQKASFDEFFLIQTETFKRYTDKKIGDKLVLIRMQGGQQYLIL
                     DRM"
     gene            151..570
                     /locus_tag="JD016_19470"
     CDS             151..570
                     /locus_tag="JD016_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HAU5001782.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLNMERNKVDGICDD
                     VEALKQTIFLILNTERYEHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNVKGKVHCKFTVHSKYGNIKAEKVVSV"
     gene            571..>1175
                     /locus_tag="JD016_19475"
     CDS             571..>1175
                     /locus_tag="JD016_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HAU5001783.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATNTIAKGVFNIDIPLDSRFNLGE
                     YNYTATERISEGIYKMKCETAGPIFELGQLIPIEYIDGLETAQLTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKIQDVGGVKVYPVW"
BASE COUNT          464 a          127 c          226 g          358 t
ORIGIN      
        1 atatagaaca aaaagcatct tttgatgaat tctttttaat acagacagaa acatttaaaa
       61 gatatacaga taaaaaaata ggagataaat tagtattaat taggatgcaa ggaggacagc
      121 aatatttgat tttagatagg atgtgataga gtgttaccaa gtgataattt agattatgat
      181 attgaagatg tatcaataat taattttgat gtaaggcaag aaccaagtaa gacatttaaa
      241 ttaaatatgg agagaaataa agtagatggt atttgtgatg atgttgaagc attaaaacag
      301 accatctttt taattttaaa tactgaaagg tatgagcacc tcatttattc ttggaattat
      361 ggagtcgagt tgaacgacct tattggagaa cctatatcct ttgtaatccc cgaacttgaa
      421 agacgaatca aagaagcact aattcaagat gatagggttg aaaatgtaga taattttgaa
      481 tttcaaaatg taaaaggaaa agtacattgt aagttcacag ttcacagtaa atatggaaat
      541 ataaaagcag agaaggtggt gagtgtataa ttgtttgagt taatgacatt tgaaaatata
      601 attaaaagaa tgttagatag tgtaccagat acttttgata aaagggaagg ttctataata
      661 tataatgctc ttgctcctgt tgctatagaa cttacagaaa catacattgc catggatgaa
      721 ttactagacc aaacctttgt agatactgct agttattact atttagagaa aagatgcaaa
      781 gagcgaggaa tcacacctct tgaagccact aatacaattg caaaaggagt ttttaacata
      841 gatattcctc ttgattcacg ctttaattta ggtgagtata attatacagc aactgaaagg
      901 attagtgaag gaatatataa gatgaaatgt gaaactgctg gacctatatt tgaacttggt
      961 cagttgattc caatagaata tatagatggg cttgaaactg ctcagttaac tgaaatatta
     1021 attaatggag aagatgaaga atcagaggac agtctaagac aaagatacta tgatagttta
     1081 aattcacaat catttggtgg aaatatacaa aattataaag atgaagttaa caaaatacaa
     1141 gatgttggag gagttaaagt ttatcctgtt tggaa
//
LOCUS       DACXMZ010000404         1169 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.405, whole genome shotgun sequence.
ACCESSION   DACXMZ010000404 DACXMZ010000000
VERSION     DACXMZ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.405"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>1169
                     /locus_tag="JD016_19480"
     rRNA            <1..>1169
                     /locus_tag="JD016_19480"
                     /product="16S ribosomal RNA"
BASE COUNT          299 a          264 c          357 g          249 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtactag gtgtcggggg ttaccccctt
      601 cggtgccgca gctaacgcat taagtactcc gcctgggaag tacgctcgca agagtgaaac
      661 tcaaaggaat tgacggggac ccgcacaagt agcggagcat gtggtttaat tcgaagcaac
      721 gcgaagaacc ttacctaagc ttgacatccc aatgacatct ccttaatcgg agagttccct
      781 tcggggacat tggtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg
      841 gttaagtccc gcaacgagcg caacccttgt ctttagttgc catcattaag ttgggcactc
      901 tagagagact gccagggata acctggagga aggtggggat gacgtcaaat catcatgccc
      961 cttatgctta gggctacaca cgtgctacaa tgggtagtac agagggttgc caagccgtaa
     1021 ggtggagcta atcccttaaa gctactctca gttcggattg taggctgaaa ctcgcctaca
     1081 tgaagctgga gttactagta atcgcagatc agaatgctgc ggtgaatgcg ttcccgggtc
     1141 ttgtacacac cgcccgtcac accacggga
//
LOCUS       DACXMZ010000405         1133 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.406, whole genome shotgun sequence.
ACCESSION   DACXMZ010000405 DACXMZ010000000
VERSION     DACXMZ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.406"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>1133
                     /locus_tag="JD016_19485"
     rRNA            <1..>1133
                     /locus_tag="JD016_19485"
                     /product="23S ribosomal RNA"
BASE COUNT          299 a          250 c          342 g          242 t
ORIGIN      
        1 ggatgtgatg gggagcgaaa tttagtagcg aagtagctga tttcacactg tcaagaaaag
       61 tctctatcga ggttaaaggt acccgtaccg caaaccgaca caggtgggtg aggagagtat
      121 cctaaggcca gcgagagaac tgttgttaag gaactcggca aaatgacccc gtaacttagg
      181 gataaggggt gccaccatca ggtggccgca gagaataggc ccaagcgact gtttaccaaa
      241 aacataggtt tctgctaagt cgcaagacga tgtataggag ctgacgcctg cccggtgctg
      301 gaaggttaag gggatctgtt agagcaatcg aagcagtgaa cttaagcccc agtaaacggc
      361 ggccgtaact ataacggtcc taaggtagcg aaattccttg tcgggtaagt tccgacccgc
      421 acgaaaggcg taacgatttg ggcactgtct caacaacaga ctcggtgaaa ttgtaattcc
      481 ggtgaagatg ccggatacct gcgacaggac ggaaagaccc catggagctt tactgtagct
      541 tgacattggg tcttggtact acatgtacag gataggtggg aggctttgaa accaggacgc
      601 cagttttggc ggagccatcc ttgggatacc acccttgtag tactgggact ctaaccatag
      661 gccatgaatc tggtcttggg acactgtcag gtgggcagtt tgactggggc ggtcgcctcc
      721 caaaaggtaa cggaggcgct caaaggttct ctcagtacgg tcggaaatcg tacgtagagt
      781 gtaaaggcaa aagagagctt gattgcaaga catacaggtc gagcaaggat gaaaatcgga
      841 cttagtgatc cggtggttct gcgtggaagg gccatcgctc aacggataaa agctaccctg
      901 gggataacag gcttatctcc cccaagagtc cacatcgacg gggaggtttg gcacctcgat
      961 gtcggctcat cacatcctgg ggctgtagta ggtcccaagg gttgggctgt tcgcccatta
     1021 aagtggtacg cgagctgggt tcagaacgtc gtgagacagt tcggtcccta tccgtcgcag
     1081 gcgtaggaaa tttgagaaga cctgtcctta gtacgagagg accgggatgg acg
//
LOCUS       DACXMZ010000406         1129 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.407, whole genome shotgun sequence.
ACCESSION   DACXMZ010000406 DACXMZ010000000
VERSION     DACXMZ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.407"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            16..1056
                     /locus_tag="JD016_19490"
     CDS             16..1056
                     /locus_tag="JD016_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAU5001784.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          445 a          138 c          189 g          357 t
ORIGIN      
        1 ttaggaggaa acaatatgaa tttaaattta actaatacta taaatttaat gaaaaaatat
       61 ttggacattc caagcccagc tggatatact gaaaatgcag ttttagaaat caaaaaagat
      121 tttgaaaatt taggtcttga cactattctt acaaaaaaag gtgcacttat agctactctt
      181 cctggtgaag atgattcaaa tcaaataaca atatctgcac atatggatac acttggagct
      241 atagtaaaag aaatatcatc tgatggtact cttaaatacc ataaagttgg tggtggatgt
      301 tgggcagcta tagagggaga aaactgtact gttataacta gaaaaggtaa aaaaatcaga
      361 ggaagtgttg tatttaagta tgcttctact catatatatg gtcaaacaaa agctgccact
      421 gaaagaaatg aagaaacaat gattattaga cttgatgaaa aagtatttac aaagcaagat
      481 gtattagatt taggaataag tgttggggat tttgtatgcc tagaccatag atttgaggct
      541 acagaatctg gttttataaa atctagatat atagataata aatcagctgt agcaatggtt
      601 cttgaaatat gtagatattt taaagaaaac aatttaacac ctaagcatac tactaacttt
      661 tatataagta attatgaaga aattggacat ggtgtttcta aatgtttacc tgaaaaaaca
      721 aaagaatttg tagcaataga tatagcacct actggaccaa gtcaaacatc tagtgaacat
      781 ggtgtaacta tagttgcaaa agataattta tcattatatg attttaattt aagaaacaag
      841 cttgtcgatg ttgcagaaga caataattta gactacgctg tcgatgtatt tactttctat
      901 ggttcagatg cttcagaagc tattcgttgg ggagaagatg ttcaaatagc atgtgttggt
      961 cctggaacaa acaatagtca ccattatgaa agaacacata tagaagctat tgaaaataca
     1021 ctaaaattac ttataaatta tatgcttaaa gaataattaa ttatatattt taccaaagtc
     1081 aattcttaac aaataattaa ctttaatttt tatttttata caaaaattg
//
LOCUS       DACXMZ010000407         1115 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.408, whole genome shotgun sequence.
ACCESSION   DACXMZ010000407 DACXMZ010000000
VERSION     DACXMZ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.408"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(466..642)
                     /locus_tag="JD016_19495"
     CDS             complement(466..642)
                     /locus_tag="JD016_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001785.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT          440 a          122 c          131 g          422 t
ORIGIN      
        1 aaacttatat aaataaaatg cttgtttttc ttgtattatt tatataaata ttgaaatttg
       61 tctagttata cgtttttatt gaccagttat gcagttgata ttgaatattc taaatattgg
      121 tgttatttta taggtggttt aaacatatta gggggatttt atatatgatt tgtatgattt
      181 atagagaatg tataaaatag taaaggttta gagccatgtt tagtggattg cagaatatgt
      241 atatagaatg attcttgtaa attaagttag acataggaaa tatataattt gtattggatg
      301 attaatttta tggtgtatgc ttttaacttc tataatggga aagtaaagat gtactcccaa
      361 tacatcttta ctttagcaga agttatagtt acttataaag tacaaaaaat tatatattta
      421 ttagagtaga taaatctaaa aaggtttttc tagaaaaatt tagttttaat tgtatctact
      481 tactaataat aagacttgat gtaagcaggt ttcaactacc gtttctccat attctgatat
      541 aaaatcatct aactgtgtat ctaaaaaatt acaataatca tatgaactac tgatgtaagc
      601 agttagtata tgtgtacatt tgtctatttc tccaataaac atattatcca tccccctaaa
      661 aaatttagta aaaataaaaa gtcgcattct attagtcaat taacataatt tataaaattt
      721 tttagtacta gtttctataa cttaactcct taaggttaaa tattcccagt tttaccaatt
      781 tagttttatt ctacaagtat ataatactag aagatgctcc attattcaac atgcttgttt
      841 gagaatagaa aatattctaa aattataatt gtttttaaaa ataagattca agataaataa
      901 agttatatgc aactatttaa taatctttcc ataataagga cagtaggttc tattcttaaa
      961 aaactccatc tttaaattta aagaataact acaatttgaa caacaataaa atttccaatc
     1021 aataaaaata tcaaaaataa taaaaataat tgaaaatgta taatttccca gaaaaataat
     1081 aaatgtaact ccaataataa acacacctat aaatt
//
LOCUS       DACXMZ010000408         1111 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.409, whole genome shotgun sequence.
ACCESSION   DACXMZ010000408 DACXMZ010000000
VERSION     DACXMZ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.409"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(290..>1111)
                     /locus_tag="JD016_19500"
     CDS             complement(290..>1111)
                     /locus_tag="JD016_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001786.1"
                     /translation="TVMKEDMSEVKQEINVLKGNMSEVKQEMIVMKEDMSEVRQEINI
                     MKEDMSEVRQEINTMKEDMSEVRQEMTVMKEDMSEVKQEINVLKEDMSEVRQEINIMK
                     EDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNFMQNK
                     INNINEDMNGIKDEVSIANEKLYGIEIKIDSLESEDKSMKEIQIEQNNILGSLLHNSE
                     INKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVKLKSVK"
BASE COUNT          296 a          194 c           91 g          530 t
ORIGIN      
        1 ttagtgccaa tatttgcata catttgtata gtataaaatt tttatgttct cattgataaa
       61 attttactta tttcaagcgt tctattagat tttatcatct aaattttagg ttttattttt
      121 ataaattaat tgtatttaca taaaaaaaaa gacctaaaaa caaattttta acggtctttt
      181 ctcaattttt tattttcatt tctaaaattt ataataaata gtaagaacac tataaattct
      241 tttatataaa attcttagtt tgtggatgat aaattagtga attaaaatac tactttactg
      301 attttagttt gacaatatca ctccaatttt tactagtggc taactctact aaatttaagt
      361 ctcttcttat ttcagctata tcctctttta tagaattagt gtcacctttt atatatgcta
      421 tgttatgttc tatattatca tgagtagctt tatttatttc agaattatgt aataaacttc
      481 ctaaaatatt attctgttct atctgtatct ctttcattga tttatcttca gattctagac
      541 tatcaatctt aatttcaatt ccatataatt tttcatttgc tatggatacc tcgtccttaa
      601 ttccattcat atcttcatta atgttattta ttttattttg cataaagttt acttctgatt
      661 taacttcgga aacatcttct ttcattacag ttatttcttg tctcacttct gacatgtctt
      721 ttttcattac atttatctct tgttttacct ctgatgtatc ttctttcatt acggtcattt
      781 cttgtcttac ttcggacata tcttctttca ttatatttat ctcttgtctt acttcggaca
      841 tatcttcttt taatacattt atctcctgtt ttacttccga catatcttct ttcattacag
      901 tcatttcttg tcttacttcc gacatatcct ctttcattgt atttatctct tgtcttacct
      961 cggacatatc ctctttcatt atatttatct cttgtcttac ctcggacata tcttctttca
     1021 ttacaatcat ttcctgtttt acttctgaca tatttccttt taatacattt atttcctgtt
     1081 ttacttccga catatcttct ttcattacag t
//
LOCUS       DACXMZ010000409         1062 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.410, whole genome shotgun sequence.
ACCESSION   DACXMZ010000409 DACXMZ010000000
VERSION     DACXMZ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.410"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..653
                     /locus_tag="JD016_19505"
     CDS             <1..653
                     /locus_tag="JD016_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001787.1"
                     /translation="KNTNNKLKEVTKNNEEIVPKNLEGLSIEQLIEIRDKAIDKNLEG
                     LREFGEDSVKKRKDSILNFEFGLVAFVNKNYSNLTEKEKLIRQINIVNYENSINDIMD
                     PSGSFFELANQNYQVNEFLLKNGKDYKEWSYEGLDFLIDHLEELCKFVDFDVKDVIDI
                     IDPTIKTDLSEEQKKSLNDKLKKISSSDTLSEEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(810..1016)
                     /locus_tag="JD016_19510"
     CDS             complement(810..1016)
                     /locus_tag="JD016_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001788.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT          443 a          118 c          134 g          367 t
ORIGIN      
        1 ataaaaatac taacaataaa ttgaaagaag ttactaagaa taatgaagaa atagtaccta
       61 aaaacttaga aggtttaagt atagaacaat taatagagat tagagataaa gccattgata
      121 aaaatttaga aggtcttagg gaatttggag aagattcagt aaaaaaacgt aaagattcaa
      181 ttttaaactt tgaatttgga ttagtagctt ttgttaacaa gaattatagc aacttaacag
      241 aaaaagagaa attaataaga caaattaata ttgttaacta tgaaaactct ataaatgata
      301 taatggatcc ttcaggttca ttctttgaat tagcaaatca gaattaccaa gtgaatgagt
      361 ttctgttaaa aaacggaaaa gattataaag aatggtcata tgaaggtcta gattttctaa
      421 ttgatcatct agaagaactg tgcaaatttg ttgattttga tgtaaaagat gtaatagata
      481 ttattgatcc tacaataaaa acagatcttt cagaggaaca aaagaaatct ttgaatgata
      541 aattaaagaa aatatcttca agtgatactt taagtgaaga agttaaaaag gagataaaaa
      601 actgggaaaa caatttaaat aatttaaata tgaataaaaa taattctaat taattttctc
      661 ataagaaaaa tgcttatatc gtgataatgt gaaatataca taattataaa caaaaaaggg
      721 gctgtgacac taaaaaatag tgctacgccc ttttttattt gtaataatca tcaatacaaa
      781 ttattttatt atatgtaatt atttatgtct taaccaattt tatcgaaaat aacagtcttc
      841 tcttttaaag agatttttcc tacttgatat ccatattcat ttaattcctt tttatatttc
      901 aaaaacgaat ggtctatctc tatcccagca atatctttta tttcttcaaa agttaattta
      961 aacgattgac tattgttttt cttaacatat tcccataaag aactatattt actcatctta
     1021 tatcactcct tttaaaatca tatcttatct attaatttat at
//
LOCUS       DACXMZ010000410         1057 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.411, whole genome shotgun sequence.
ACCESSION   DACXMZ010000410 DACXMZ010000000
VERSION     DACXMZ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.411"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            152..>1057
                     /locus_tag="JD016_19515"
     CDS             152..>1057
                     /locus_tag="JD016_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAU5001789.1"
                     /translation="MLNLQSKWVNFLKPTYVSDEIKRKKLQVIIFFSITYGLSYSLGL
                     LAICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFYSSVILFFIIYFFIF
                     IIESLKLISINQFQLMYLMLILISSLCVIVYSYKITSLYIFKNFKIGILLFFYFLFSQ
                     IIFGLIISDNQFDYKNFLNYILLPIISLVYIYPFLCEEYGWRSFLQSILFDRFGKKIG
                     IVMVGTCWSLWHLPLQFTLYSPQTPIIGAIAHLIYGIGLSIFLGYVYMKTKNIWFCSI
                     MHVLINSLGLIFNDSEIIINYHSIIQ"
BASE COUNT          376 a           93 c          135 g          453 t
ORIGIN      
        1 atacatattc aaaaaaagaa ttataagagt ttagtcagca gtcgtgtaag taataaggac
       61 tttataaata tatagaaata taatgatatg agtataagta gaaaaatttt atgattttta
      121 caattatttt ttataaaaaa ggaggttatg tttgttgaat ttacaatcaa aatgggtaaa
      181 ctttttaaag ccaacatatg tcagtgatga aataaaaaga aaaaaactac aagttataat
      241 atttttttct ataacttatg gtttatctta ttcactaggt ttattagcaa tatgttttaa
      301 tcatttaata gattcagaaa ttttagctca atttatgatg atattgccat tatcttcagt
      361 ttctattgca aaattttaca cagagggaag aaataatgat aaatataatt tctattcatc
      421 agtaatatta ttttttatta tttacttttt tatatttatt attgaatcat taaagttaat
      481 aagtattaat caatttcaat taatgtatct tatgttaatt ttaataagta gtttatgtgt
      541 tattgtttat tcttataaaa taacaagttt atatattttt aaaaatttta agataggtat
      601 tttgctattt ttttactttt tgttttctca aattattttt ggattaataa taagtgataa
      661 tcaatttgat tataagaatt tcttgaatta tattcttctt cctataatat cattagtata
      721 catttatcct tttttatgtg aagagtatgg ttggagatct tttttgcaaa gtattttatt
      781 tgatagattt ggcaaaaaaa tagggattgt aatggtaggc acttgttgga gtttatggca
      841 cttaccttta caatttacac tatatagtcc tcaaactcca ataattggag ctatagccca
      901 tttgatatat ggtattggac tttctatatt tttaggttat gtttatatga aaactaagaa
      961 catatggttt tgctctataa tgcatgttct aattaatagc ttaggattaa tttttaatga
     1021 ttctgaaata attattaatt accatagtat tatacaa
//
LOCUS       DACXMZ010000411         1056 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.412, whole genome shotgun sequence.
ACCESSION   DACXMZ010000411 DACXMZ010000000
VERSION     DACXMZ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.412"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(181..966)
                     /locus_tag="JD016_19520"
     CDS             complement(181..966)
                     /locus_tag="JD016_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001790.1"
                     /translation="MNFENDFITSEFRKKYIGLVLDNAQNINKVYTTTFPDKEIIKQV
                     IDKDEKDILIFSHHAMGYIASDEGFPFHDIPLSYMEEMKNRRISFYVLHSPLDNYSDY
                     STSVSFAKAMGLEIVKPFCKYDDKIEVGVICKTSLKSIEEIEELIKKSVGHDVKLYDY
                     GDSVLKDGLVAIAAGGGSYPFVAREVAELGINLYITGFTKPLKHFEPVLEFHQIAKDN
                     LINVIGATHYSTEKFACMGMVDYFKNLGLESEFLQGKYYLEDL"
BASE COUNT          384 a          169 c          104 g          399 t
ORIGIN      
        1 tatcgaaaaa taatttaaaa gacatcaaaa ctaaatcttc taatcagcaa ttaaaagtct
       61 attttcttag tttaaaaaat aaagatagat tttttcaaaa cactagataa ataggtaaaa
      121 aaataaacag ttagtaaaat aaatttttac taactgttta tttttattgt ttaatatgtc
      181 ctataaatcc tctaagtaat attttccttg caaaaattct gattctaaac ctagattttt
      241 aaaataatcg accatgccca tacacgcaaa tttttctgta gaataatgag tagcacctat
      301 aacattaatt aaattatctt ttgctatttg atggaactct aatactggtt caaaatgttt
      361 taaaggctta gtaaacccag taatatatag atttatacca agttcagcaa cttctcttgc
      421 tacaaatgga taacttcctc cccctgctgc aatagcaact aaaccatctt ttaatacaga
      481 atctccataa tcatacaatt tcacatcatg accaacagat tttttaatta attcctcaat
      541 ctcttctatt gattttagtg atgttttaca tataactcct acctcaattt tatcgtcata
      601 tttacaaaat ggtttgacta tttctaaacc catagctttt gcaaaactaa cagaagttga
      661 atagtcacta tagttgtcta gtggagaatg taatacataa aaagaaattc tacgattttt
      721 catctcttcc atataagata gtggtatgtc gtgaaatgga aaaccttcat ctgatgctat
      781 gtatcccatt gcatgatgtg aaaaaattaa aatatctttt tcatctttat ctataacctg
      841 cttaattatt tctttatcag gaaaagtagt tgtgtatact ttattaatat tttgagcatt
      901 atctaatact aatcctatgt atttttttct aaattctgaa gtgataaaat cattttcaaa
      961 attcataaaa ctccaatcat cattaatatt tttaatatta aattctaaat ctaatttttt
     1021 ataaagttca attgccttaa taataaccac tccttt
//
LOCUS       DACXMZ010000412         1054 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.413, whole genome shotgun sequence.
ACCESSION   DACXMZ010000412 DACXMZ010000000
VERSION     DACXMZ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.413"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            124..912
                     /locus_tag="JD016_19525"
     CDS             124..912
                     /locus_tag="JD016_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAU5001791.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
BASE COUNT          405 a          125 c          190 g          334 t
ORIGIN      
        1 agtgttttgt aaataaaatg taataaataa ttaattaaca aatataaatt ttaaaataag
       61 ttagatattt agaaaaatcg aaatttgtag gatttaaagg acatattcaa ggaggagtta
      121 ataatggaaa aattacaagg aaaaattgca gtagttactg cggcaacaaa aggtattgga
      181 ttagcatcag cagagatatt agcaaaaaat ggagcaactg tgtacttagc agctcgttca
      241 gaagaattag ctaatgaagt tataaataaa ataagtgcag aaggtggttg tgctaagttt
      301 gtttacttta atgctcgtga agaagagacc tttacctcaa tgatagaaga ggtagttaaa
      361 aaagaaggta agatagatat acttgtaaac aattttggtt caacaaaccc ttctcttgac
      421 aaagaccttg taactgggga tacagataat ttctttgaca cagtaaatac taatttaaaa
      481 agtgtgtatt taccatgtaa agcagcaatt cctcatatga taaagaatgg aaaaggaagt
      541 atagtaaata tatcaagtat aggttcagta ttacctgatt tatctagaat agcttactgt
      601 gtatcaaaag cagcaatcaa ctcattaact caaaatatag ctacacaata tgctaaagac
      661 aatgttagat gtaatgctgt acttccaggg cttattgcaa ctaaagcagc attagacaat
      721 atgtctccag agttcataaa agaattttta aagcatgttc ctttaaatcg tataggggaa
      781 ccagatgata tagcaaaagc tgttctattc tatgctagtg atgactcatc atttataaca
      841 ggggatttac ttgaagttgc aggaggattt ggtttaccta ctccacaatt tgcagataat
      901 atattaggat aattaaatta ctaatatgca atgaaagctt atgcattagt tatagtagaa
      961 atgatatttt tataaaggcc atagatataa tttatattgg aaaggtttga ggttcaaaat
     1021 tccaagttaa atttatttat ggtctttatt ttgt
//
LOCUS       DACXMZ010000413         1040 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.414, whole genome shotgun sequence.
ACCESSION   DACXMZ010000413 DACXMZ010000000
VERSION     DACXMZ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.414"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            1..864
                     /locus_tag="JD016_19530"
     CDS             1..864
                     /locus_tag="JD016_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAU5001792.1"
                     /translation="MMLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITG
                     LGVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMA
                     TLDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGI
                     VDCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISK
                     KIMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIH
                     EALGNGFKKIE"
BASE COUNT          373 a          115 c          158 g          394 t
ORIGIN      
        1 gtgatgttat tgaaaaaaga tagaataact ttttcagaaa ttttaatatt aattgtaggc
       61 tgtatattaa tggccatttc tcttaactta ttttttaatc cacatgcaat cgctgctggt
      121 ggtataacag gacttggagt tgttttaaat tcattattcg gtgtagaact ttggattgtc
      181 aatttacttt taaatgttcc attattcata tttgcttaca aaatactttc aaaaaaagat
      241 tgttttaaaa cggttttagg aataattttt cttaccatag cacttaagct tactgcaaat
      301 atggctacat tagatataac aaatgatatg tatcttgcaa ttatatctgg ttctatttta
      361 atgggagttg gtcaaggact tatttttaga ataaatggct ctacaggtgg aactgattta
      421 atggcactcc tactaaataa atattttcct acatttagta tacctgtttt aatgggtatt
      481 gtagactgcg ttgtagttgt tttatcaggt atagtaaata gacaggttga aattgcactt
      541 tattcaactg tggctttata cattttagta aaagttagtg acttgcttat tgaaggattt
      601 aattattcca aatcattcac tattatatct gacctttcaa aagatataag taaaaaaata
      661 atggaagatt tagatagagg tgcaactatt ctaaaaggag aaggtgcata tactggagaa
      721 aataaaaatg tacttttagt agtagttgaa aagaaagaag ttgtcgaatt gaaaaaatta
      781 gtaaaaaatg tcgaccctaa tgcttttatt ataattacag atatacatga agctttaggt
      841 aatggattta aaaaaataga ataactaaca aataaatagc aagtataatt aacatataca
      901 aattcaactt tttatatgtt atattatact tgctatttat atattataat tgttgttttt
      961 aagataaaaa ttaaatgtat caattattgt tttatacaag tatttccaaa tccgttctca
     1021 tttaaatagt atgcttagaa
//
LOCUS       DACXMZ010000414         1001 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.415, whole genome shotgun sequence.
ACCESSION   DACXMZ010000414 DACXMZ010000000
VERSION     DACXMZ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.415"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            28..>1001
                     /locus_tag="JD016_19535"
     CDS             28..>1001
                     /locus_tag="JD016_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HAU5001793.1"
                     /translation="MSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEEKREPVKKDIT
                     ISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDLTVVNLEGPLT
                     TASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDTQFILDENKIN
                     YFGLGKDVVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIESLKKKSDVVVVYFHWG
                     IELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYSLGNFCFGGNK
                     NPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL"
BASE COUNT          416 a          106 c          180 g          299 t
ORIGIN      
        1 aaaaataaat tagtttacat atgtattatg agtctattgc tatttgtagt aggatgttct
       61 aataatccaa ataaggaaag taccattaaa aaagactcta agaataacca aagccaagtt
      121 aaagaagaaa aaagagaacc tgtgaaaaaa gatataacta taagtttcgc tggtgatgta
      181 actatgggga actataaagg ttcatcatat tatggcagtt ttgaccatga atttaaaaga
      241 caaggagaag attacaatta ttttcttaaa aatgtaaaac ctgtatttga aaaagatgac
      301 ttaacagtgg ttaatttaga aggtccactt actacagcgt caaatgcaaa agaaaaaaaa
      361 tttgcattta aaggaaagcc tggatatgta aatattttaa aatctggaag tgtagaggca
      421 gtaagtattg caaataatca ttcagaagat tattttgaag aaggaatgaa agatacacag
      481 tttatattgg acgagaataa aataaattat tttggattag ggaaagatgt tgttgtagat
      541 gtcaaaggaa ttaaagtggg tctattaggg tataatggat tatcaactga atataatgaa
      601 aaaaatttaa aacaaatgga agatgatatt gagtccttaa aaaagaaatc ggatgttgtt
      661 gtagtgtact ttcattgggg gatagaatta gattattatc cagataaaaa acaaaaagat
      721 tttgctcatt atgctataga caagggagca gatttagtta tggggtctca cccgcatgtg
      781 attcaaggaa tagaaaagta taaaaataag tatatagctt atagtctagg gaatttttgc
      841 tttggaggaa ataagaatcc ttctgataca gatagttata tatatcaaca aacctttact
      901 ttttcagata ataaattaac ttcaattaaa gagccaaata taataccaac ttcaataaca
      961 tctagtaact ccagaaataa ttatcaacca aaaatattgg a
//
LOCUS       DACXMZ010000415          985 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.416, whole genome shotgun sequence.
ACCESSION   DACXMZ010000415 DACXMZ010000000
VERSION     DACXMZ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.416"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            123..263
                     /locus_tag="JD016_19540"
     CDS             123..263
                     /locus_tag="JD016_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001794.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(260..631)
                     /locus_tag="JD016_19545"
     CDS             complement(260..631)
                     /locus_tag="JD016_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAU5001795.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
BASE COUNT          374 a          105 c          120 g          386 t
ORIGIN      
        1 aaattttgga taatcaaaat atattttata aaatcaaaat acaattacat aaactttatt
       61 aaaaatttgt tgggaatttc tgactaacat taagaacgta agtttgttgg gggatggtat
      121 atgtggaaaa gtcaaacact actgttacaa aaaaagaaaa aattgaagat ataggaatga
      181 aattagaaca tttattgagt gaagatatta ctttttatga attattactt gagattttgt
      241 ataaagaaaa agaagagaat taattctctt ctttaaggaa tctttttaaa taggcattat
      301 caatcatttt ttctatagct tctctatctt ctttttcaag agaatatatt ttttctaaga
      361 gcttttttgt tttttcactt actgataagt tctctatgat taatttatct tctttgctta
      421 tttttgtatt atcatttacg cctaataaat aatctgttga tgtttctaat gcttttgcta
      481 atctaactat tatttcactt ttaggttctc ttaaattatt ttcataacgt gacaagctag
      541 cctcagttat accgacttta gtagcaagtt ctttttgatt tatatctaat tcttttcgta
      601 gatttgctat tctatctcct aaactttcca tatgtacatc actccttacc gaaattataa
      661 gttattttga taaaaagtaa aataaaatta ccaaaaatat aaaaaaaaag ggttaaaata
      721 ataaaatata ttgaaaatta ccatttgtat atgtataata tgaattatac aaatggtaat
      781 ttgatgagag gtggtatgtt gaaataagaa actgaattaa ttaataaata tatagaaagt
      841 tttgaaaacc cttaattgag atataaacaa tatgtacaaa attttttgca agtaaaatta
      901 ccaaatgtat aaaataaatc agtagcatag ataattttat gctttaatac attagattat
      961 ctaatttagt tataatggtc aaaaa
//
LOCUS       DACXMZ010000416          964 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.417, whole genome shotgun sequence.
ACCESSION   DACXMZ010000416 DACXMZ010000000
VERSION     DACXMZ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.417"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     repeat_region   49..540
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=116..144
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
BASE COUNT          380 a          126 c           88 g          370 t
ORIGIN      
        1 atataactag ataatcataa gataacacat taaaagaaaa ttataaaaac ttacattctg
       61 tatagttaat ataaaacata agtaatataa atttatgtaa agcaataaaa gatggattta
      121 cattccatat agttaatata aaactttaaa tcatctttat gaacttcaaa agtaggaagc
      181 gtatttacat tctatatagt taatataaaa ctagtaaaat caaactatga aagaataaat
      241 gtttttaata tttacattcc atatagttaa tataaaacat caatactttg atgcagtttt
      301 taactagttt ttcaatttac attccatata gttaatataa aactgataag attcttgtta
      361 ctgctgttcg aaagttcaaa tttacattcc atatagttaa tataaaacgt ttgaggattt
      421 attagcaact catagtgaaa agtatattta cattccatat agttaatata aaactaaagt
      481 ttgaggtgag ttttttgatt tatttttatt catttacatt ccatatagtt aatataaaac
      541 accaaaataa acttagtatt ttcaacactt acacgtatac aactctctta aatttgcagt
      601 gaacgagcaa tagtgcaatt gataacgctt atcatgcact ctcaacgcct tatatttcaa
      661 ctgttacgcc aaattttttc acaaatatcg ctcactgcaa aatttctata attttattat
      721 atcataaata tattactttt gaatattgat accaatttat accataataa aagcaaaaaa
      781 acgtaatcta tctacattag atttaaattc tataacttaa agttttttac ataatattta
      841 tgcttgttta atcaatctaa tatatcattt ttgtatattg cttttaatgg tcttttttct
      901 taaataaata tctatctgaa caagcatagt ttaattaaaa acttttgtat tcccttaaaa
      961 ctgt
//
LOCUS       DACXMZ010000417          940 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.418, whole genome shotgun sequence.
ACCESSION   DACXMZ010000417 DACXMZ010000000
VERSION     DACXMZ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.418"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(164..655)
                     /locus_tag="JD016_19550"
     CDS             complement(164..655)
                     /locus_tag="JD016_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HAU5001796.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKELSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          347 a          148 c           83 g          362 t
ORIGIN      
        1 taatataaat atttataaag acataagaca aatttaccta cagatttaaa tttaacttat
       61 atattttaaa aggctaatat aatatgtatt ttcttctata caatcaaaag gcttcatatc
      121 taagatatgt gatgacacta atattcacaa tttttagtaa ttatcacatt ataaaaaata
      181 ttacagctag tccatcatca gatgcttctt gtataaactt tttaagctca attagatttg
      241 cccatacata ttcaaaaaaa tcatcttcac tctcatcatt cataacagga taaatattat
      301 tttctaacat ctcattaata ttaaatcttt ttctaaatgc ttgttcttcc aaactgttca
      361 ttgcttttga tagttctttt acatcatttg ctgtaatcaa catgacagga aattctccat
      421 caatttccat aagtatgttt ccacttagta ctaatttgct aaatatatta tcatcatctc
      481 caccaaaagg acaacctgtc aaagtaaagt gaatggcatg ccatgcttta tcaatattga
      541 tagtactatt ttcgttggca tcttcaaaaa caaaatcttg taaattttct tctgcttgct
      601 gtaatttaaa aacattctct tcacttattt tagtataaca tcctatcatc cccataatta
      661 cattcctcct aagtagcgtt tttttaattg taacttataa ttaaacttag ttacaataaa
      721 atatattgag aaaagtatta tttcatctta aatttaaata cctatattat caactaaaat
      781 tctgtgttta tagcgtaagt attattattt tttcctaaaa atcaaatgaa tcagaagttc
      841 tataggcaat attgactata aataagaact attactttta aaaaaaaatt taagaaatta
      901 tactgtagaa tcatgctcta cttattagct ttaaataaaa
//
LOCUS       DACXMZ010000418          936 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.419, whole genome shotgun sequence.
ACCESSION   DACXMZ010000418 DACXMZ010000000
VERSION     DACXMZ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.419"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(156..839)
                     /locus_tag="JD016_19555"
     CDS             complement(156..839)
                     /locus_tag="JD016_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAU5001797.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT          370 a          149 c           75 g          342 t
ORIGIN      
        1 taattaattt atacactgct tactatactt aaaatacttc tattaactta tacattgctt
       61 actatatttt aaattcttta attaatctat acaatatttg ctatatataa agtatatcaa
      121 ataacagctt catttttttg taaatttaca gaaagttaat tttcattaaa tgaatcatct
      181 ttattgataa acataattcc aaagaccact gccaaaataa aaaaacatat tagtattgat
      241 ttttccacga aaatttttgt aaaaaaagtt cctaaaatag ctccagatat aaagaatata
      301 ataatagcat aatactgtaa actctttttt atcataaaaa tattcttagt atgtatataa
      361 tgaaataaag tttctgtagc acttcttaaa tttccagtac acattgtcgt tgcaaaacta
      421 ttcccattta cttttctaaa actctctact tgcattgaac aaataaaaga aatagcaaca
      481 tttacaaaca tattaaatct tcctctagga ataaatgaaa caataagtat tgtaatcatt
      541 tcaattataa tgataacctg acgccaatga atctttgtct tattatttgt aaaatacttt
      601 cttatcttct cggctataaa tacacctaac ataaatgcca aaataggaat caaataataa
      661 aatgattcct ttacattacc ttctgctata ttaagcccaa ataaaactat atttccagtt
      721 tgtgcattag caaagacttt acctcttacc acataagtat aaatatccaa aaatcctcct
      781 gttattgcca acaaagaaca taaaaagaat gattcagaca tctgactctt gcttgtcata
      841 tataaacctc tcgctttcta atgatagata caaaatcaaa atcattttag attctatttt
      901 tcattgatat aaaaaaatta tttatattta aatatt
//
LOCUS       DACXMZ010000419          935 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.420, whole genome shotgun sequence.
ACCESSION   DACXMZ010000419 DACXMZ010000000
VERSION     DACXMZ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.420"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            307..777
                     /locus_tag="JD016_19560"
     CDS             307..777
                     /locus_tag="JD016_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HAU5001798.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKEVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
BASE COUNT          450 a           62 c          159 g          264 t
ORIGIN      
        1 aaaaaataaa agattgatat aagatactga agaggtagaa acaaaagtat taatagaaga
       61 tattgaaaaa ataaaaacaa aagattgata gaaagtactg aatagataga gataaaagtg
      121 ttaatagaag atattgaaaa aataaaagat tgatataaga tactgaagag gtagaaacaa
      181 aagtgttaat agaagatact aaaaaataaa aaataaagga ttaataaaag gtactgaaga
      241 gatagagaaa aaaatgttaa tagaagatac tgaaaaaata gaaataaaaa attaggagga
      301 aaatttatga aatatcaggt ttcattatta gcagtgaagg atattgaagt atcaaaaaaa
      361 ttttacaaag aattatttga gcaagaaatt gaattggact taggtaaaaa tgtaacattt
      421 aaaggaggat ttgctataca agaagacttc gcatggctta cagggattaa tccagataca
      481 attatacaaa aatcaaataa tatggaactt tattttgagg ttgatgattt tgacgaattt
      541 attgaaaaac tcaattatta taaagaggta gagtttgtcc ataaaccact aaaacatgaa
      601 tggcaacaaa gagtagtgcg tatttatgac ccagataagc atattattga gataggagag
      661 tctatggaag ttatagctag aagatattta tctgagggat attctgtaga agaaacttct
      721 aaaataattc aacatccaat taaatttgta aagcagtgtg aaaatagtaa ggaatgattg
      781 tttaaattat aattatttag tagaaattaa ttgtggtaga actaattcaa gataatatat
      841 aggactagta tttgacaaat gtcataaatt aaattaaaat aaagaaaata ttaataaaga
      901 aaaaataaat ataatattaa aatatattaa taaat
//
LOCUS       DACXMZ010000420          927 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.421, whole genome shotgun sequence.
ACCESSION   DACXMZ010000420 DACXMZ010000000
VERSION     DACXMZ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.421"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            38..742
                     /gene="cwlD"
                     /locus_tag="JD016_19565"
     CDS             38..742
                     /gene="cwlD"
                     /locus_tag="JD016_19565"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HAU5001799.1"
                     /translation="MRKYIKHIIFSFAMICLVVVSIFEIKNISEDVIKYMPVTNKTII
                     LDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGLVILTREDDSSLYKEEN
                     NKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELS
                     KCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGFLSNEKECKLLTDETYQ
                     EKIAWAIYIGIQKYLS"
BASE COUNT          406 a          101 c          148 g          272 t
ORIGIN      
        1 tagtgttttt ataagtatac atatacaggg tgatgttgtg agaaagtaca taaaacatat
       61 aatttttagt tttgcaatga tatgtctagt agtagtatcc atatttgaaa ttaaaaatat
      121 ttctgaagat gttatcaagt atatgccagt aacaaacaaa actataattt tagatgctgg
      181 tcatggaggc attgatccag gtgcattaaa taaggataag agtacatctg aaaaggatat
      241 taacctagca ataacactta agcttagaga gcttatagaa tcaagtggtg gccttgtaat
      301 attaactcga gaagatgaca gtagccttta taaagaagaa aataataaaa ctacaagaca
      361 aaaatataat gagaatttga aaaatagaaa agaaataata tctaattcta atgccaatat
      421 gtttgtctct atacatctta atgcatttga acaatcaaaa tactatggag ctcaaacttt
      481 ttatcctaaa gacaaacaag acagtaaaga attatcgaag tgtattcaag aagaacttaa
      541 aagagtagta gataagacaa acaacagaga agtcaaacca agggatgata tatatctttt
      601 gaaagaaaat aatattccat cagtactgat agaatgtggt tttttatcaa atgaaaaaga
      661 gtgtaaactc ttaactgatg aaacatatca agaaaaaata gcatgggcaa tctacatagg
      721 aatacaaaaa tatttaagtt gaaataacta taaaaatagg tctaaatatg taaatagctt
      781 gtattaaagc agttaaaatg cattaatata ggctattttt attttgacaa aaaaatattt
      841 aaaataaaag ttaaaaagtt gttgacttag aataatatag atgatattat atatgagtgc
      901 ccaaaaggag caccaaaata agacaaa
//
LOCUS       DACXMZ010000421          914 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.422, whole genome shotgun sequence.
ACCESSION   DACXMZ010000421 DACXMZ010000000
VERSION     DACXMZ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.422"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            69..839
                     /locus_tag="JD016_19570"
     CDS             69..839
                     /locus_tag="JD016_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAU5001800.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT          299 a          111 c          190 g          314 t
ORIGIN      
        1 tattgttatt atataattat tgccataaaa agacaaggga aagtataatt tttaaggggg
       61 aaggatttat gagtaaagga ttcttgacac ctggggaaac tgcacaagca actataagtg
      121 caggcattaa aaaagcaaat atttcgacac aaaacgcaat tctattaggt ttatttggag
      181 gagcatttat aggttttggg gcattgggga gtatgatagt tagtcaaact tttggcaaga
      241 tagacccagg ggtagctagc tttttaagtg ctatggtctt tccagtaggt cttatgcttg
      301 tagtagtggc aggagcagaa ttattcactg gaaacacatt aatggctatt gcacttatgg
      361 acaaaaaaat aacattagga aaaatgttaa gaaactgggg actggtttat tttgcaaatt
      421 tagtaggttc aattttgcta gttgcacttg tgttttattc ttctacattg acaggagatg
      481 cagctacaaa agctatagct gtggcagaag ctaaggttaa ttcaactata gcatcaatgt
      541 ttttaaaagc aatactttgt aatattctag tcgttttagc tgtatggatg gctacagcat
      601 ctcaagatat aatgtcaaaa ttggcttcat gttggacagt aataatgcta tttgtattat
      661 gtggattcca acatagtgtt gcaaacatgt tttttatacc aatgggaatg gcattaggag
      721 caaatataac tatggttcaa tttataacta atttggtatt tgtaacactt ggaaatatcg
      781 ttggaggttc catagtagtt ggagggattt attatctttg ctatgtcaaa aatagttaga
      841 taaataatac gaattaaatt gccttttaaa gcattgatat cagtgatatt gatgcttttt
      901 ttaaattatt tata
//
LOCUS       DACXMZ010000422          912 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.423, whole genome shotgun sequence.
ACCESSION   DACXMZ010000422 DACXMZ010000000
VERSION     DACXMZ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.423"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            99..174
                     /locus_tag="JD016_19575"
     tRNA            99..174
                     /locus_tag="JD016_19575"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:132..134,aa:Met,seq:cat)
     gene            182..256
                     /locus_tag="JD016_19580"
     tRNA            182..256
                     /locus_tag="JD016_19580"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:215..217,aa:Glu,seq:ttc)
     gene            272..345
                     /locus_tag="JD016_19585"
     tRNA            272..345
                     /locus_tag="JD016_19585"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:304..306,aa:Gly,seq:tcc)
     gene            351..426
                     /locus_tag="JD016_19590"
     tRNA            351..426
                     /locus_tag="JD016_19590"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:384..386,aa:Val,seq:tac)
     gene            432..508
                     /locus_tag="JD016_19595"
     tRNA            432..508
                     /locus_tag="JD016_19595"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:466..468,aa:Asp,seq:gtc)
     gene            519..593
                     /locus_tag="JD016_19600"
     tRNA            519..593
                     /locus_tag="JD016_19600"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:551..553,aa:Gly,seq:gcc)
     gene            603..676
                     /locus_tag="JD016_19605"
     tRNA            603..676
                     /locus_tag="JD016_19605"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:637..639,aa:Arg,seq:tct)
BASE COUNT          253 a          202 c          215 g          242 t
ORIGIN      
        1 gaagtggcgg aactggcaga cgcacaggac ttaaaatcct gcgggactta cctctcgtac
       61 cggttcgatt ccggtcttcg gcacttataa aacaatatcg cggggtggag cagttggcag
      121 ctcgtcgggc tcataacccg aaggtcgtag gttcaagtcc tgcctccgca accattaaaa
      181 cggctcgttg gtcaagaggt taagacaccg ccctttcacg gcggtaacag gggttcaatt
      241 cccctacgag tcaccaaatt caattaaata tgcgggtgta gctcaatggt agagttctgg
      301 ccttccaagc cagctgtgag ggttcgatcc ccttcacccg ctccaaaaat gggaccatag
      361 ctcagctggg agagcacctg ccttacaagc agggggtcac aggttcgagc cctgttggtc
      421 ccaccattcg cggcctggta gttcagttgg ttagaatgcc agcctgtcac gctggaggtc
      481 gagggttcga accccttcca ggtcgccaaa ttaaataagc gggaatagtt cagtggtaga
      541 gcgcaacctt gccaaggttg aagtcgcgag ttcgaatctc gtttcccgct ccataataac
      601 ttgggtgcat agctcagctg gatagagcaa cgcccttcta aggcgtgtgt ccggggttcg
      661 aatccctgtg cgctcattaa gaaaagttta ctataaatta tagtaaactt ttatattgtt
      721 taaaaagaca caggaaacat gtatattacc tgtgtctttt tatttataaa ctttaaacta
      781 tgtaaaaatt acagtatatt ctaatagagt tacagtatat tcattaatat tgtaaacata
      841 cggagggtaa tgtataatag tctacaagat gtttatgaag gcaatcagtt aaatataata
      901 aatataacta at
//
LOCUS       DACXMZ010000423          888 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.424, whole genome shotgun sequence.
ACCESSION   DACXMZ010000423 DACXMZ010000000
VERSION     DACXMZ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.424"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            49..137
                     /locus_tag="JD016_19610"
     tRNA            49..137
                     /locus_tag="JD016_19610"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:83..85,aa:Ser,seq:tga)
     gene            155..245
                     /locus_tag="JD016_19615"
     tRNA            155..245
                     /locus_tag="JD016_19615"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:189..191,aa:Ser,seq:gct)
     gene            254..330
                     /locus_tag="JD016_19620"
     tRNA            254..330
                     /locus_tag="JD016_19620"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:288..290,aa:Pro,seq:tgg)
     gene            416..491
                     /locus_tag="JD016_19625"
     tRNA            416..491
                     /locus_tag="JD016_19625"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:449..451,aa:Trp,seq:cca)
     gene            552..628
                     /locus_tag="JD016_19630"
     tRNA            552..628
                     /locus_tag="JD016_19630"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:586..588,aa:Pro,seq:tgg)
     gene            635..711
                     /locus_tag="JD016_19635"
     tRNA            635..711
                     /locus_tag="JD016_19635"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:669..671,aa:Ile,seq:gat)
     gene            715..790
                     /locus_tag="JD016_19640"
     tRNA            715..790
                     /locus_tag="JD016_19640"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:748..750,aa:Phe,seq:gaa)
     gene            798..874
                     /locus_tag="JD016_19645"
     tRNA            798..874
                     /locus_tag="JD016_19645"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:832..834,aa:Met,seq:cat)
BASE COUNT          212 a          205 c          255 g          216 t
ORIGIN      
        1 acttttaatc agggtgtccc gcgttcgagt cgcggataga tcaccaatgg agaggtgtcc
       61 gagtggttta aggagctggt cttgaaaacc agtgacttcg aaaggggccg tgggttcgaa
      121 tcccaccctc tccgccaaaa aaatgactga atttggagaa gtactcaagt ggctcaagag
      181 gatcccctgc taagggatta ggctgggtaa ccggtgcgag ggttcgaatc cctccttctc
      241 cgccattcaa aaacgaggtg tagcgcagtt tggtagcgca catggtttgg gaccatgggg
      301 ccgggggttc gagtcccttc acctcgacca acttaagctt ttagttcagt tggttagagt
      361 gcccaactca gggctagtaa ttctagggtt tgagtccatg aaggttattc ttcatagggg
      421 tgtagctcag ttggtagagc gtcggtctcc aaaaccgtgc gccgggggtt cgagtccctc
      481 cacccctgcc aaataaaaag gacttgaaaa aaattggatt ttagagtagt ataagatagt
      541 aaataagatt tcgaggtgta gcgcagtttg gtagcgcaca tggtttggga ccatggggcc
      601 gggggttcga gtcccttcac ctcgaccaag aaaagggcct atagctcagg tggttagagc
      661 gcacgcctga taagcgtgag gtcgctggtt cgagtccagc taggcccacc atttgcccag
      721 atagctcagt cggtagagca ggggactgaa aatccccgtg tcggtggttc gattccgcct
      781 ctgggcacca gaaatacggc ggtatagctt agttggctag agcgttcggt tcatacccga
      841 aaggtcatag gttcgactcc tattaccgct accatctaac tatatcaa
//
LOCUS       DACXMZ010000424          886 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.425, whole genome shotgun sequence.
ACCESSION   DACXMZ010000424 DACXMZ010000000
VERSION     DACXMZ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.425"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            96..860
                     /locus_tag="JD016_19650"
     CDS             96..860
                     /locus_tag="JD016_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAU5001801.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
BASE COUNT          363 a           86 c          137 g          300 t
ORIGIN      
        1 taaattaatt ttgtatttca tgttaaaata gttgatatat tgtattttat cttataatat
       61 ttactataat actatattgc gaaaagaggt agaaaatgtt tgcagaagaa agaatccaag
      121 ctatattaaa tatcctaaaa aaagaaggta gagttactgt caaagagctc agttctaaat
      181 ttaatgttac agaagattgt ataagaaaag atttaaaaaa tatagaaaag atttcttcaa
      241 taagaagaat ttatggagga gctgtactag ctagagaaac actagagaat caggatacaa
      301 aagacagaaa agaaattaat attccaacaa agaaaataat agcagaaaaa gcatttaatt
      361 taattgaaga tagagaaact atatttttag atatctctac aattaacatc ttgcttgcaa
      421 agttgttagc tgaaagtaat aaaaaagtaa cacttgttac aaatatgctg gatatactaa
      481 atgtagttac ttcaaaacca aataatttaa atataatttc cactggaggt ttattaaatc
      541 tcagtttaga cggttttgtc ggtactccaa ctatagagtt tatatcgaaa tataagtttg
      601 ataagacttt tatgggaagt tgtggagtag atgtattcaa ctcttcactt acaacttttg
      661 aaattgaaga tggtcttacg aagaaggcta taataaattc aagtaaaaaa gtatttatag
      721 ttatggaaga taaaaagttt aaatttgatg gaaatttcaa gtttgctcat ttagaagata
      781 ttagttctat aattacagaa aaaacgccta ctcctgatat agtagatatt ttatcagaat
      841 ttaatgtaaa tgttttataa gtttaattat atattttata ggattt
//
LOCUS       DACXMZ010000425          876 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.426, whole genome shotgun sequence.
ACCESSION   DACXMZ010000425 DACXMZ010000000
VERSION     DACXMZ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.426"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            134..676
                     /locus_tag="JD016_19655"
     CDS             134..676
                     /locus_tag="JD016_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAU5001802.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT          369 a           74 c          147 g          286 t
ORIGIN      
        1 gacaaatatg ttaaaaaaat gataatcacg gagaactttt accttcttca attaatatat
       61 tattattagt aataacttta aaattatatt actattatat aaaaattggc ttaaaagaag
      121 gtgaaaaaag taaatgaaaa tatgcataac agtaggtcat agtatactaa aaggtggtaa
      181 aagtacaggt gtaaatggta tagttgatga atacaattat aataaaaaat tagctcctat
      241 gttagctgag atgcttataa gtcaaggtaa tacagtagat gtaataattt gtccagaaag
      301 gcactttatg tcagaaagag aagaattttt ctatagagtt ccaaaagtaa acagtggtaa
      361 atacgattta ttagtagagc tacatttaaa taaagctgat ggaacacaat gtggtacaga
      421 agtgctttac tatggcactg aaggattaga atatgctaaa agagtatcta atagacttgg
      481 agaattattt gaaaatagag gagctaaaaa aagagaaaat ttatatatat tgagaaatac
      541 aaatccagtt gcaatacaga ttgaaagttt tttctgtgat aatgtgaatg attgtaacaa
      601 agcaaatgaa tcaggttatg attatatagc acgattgatt acagaaggta tattgaataa
      661 agatataaat atgtgagatt atggtgattt aggtattgat taataccttt taatattaga
      721 tataaataaa aatacaatgt attagtataa tttaaacttt caaaagatag attagtttga
      781 ttattgacaa gagatacctc cttgaaaata ttgatttatc agtatattta aggaggtatt
      841 taaatataaa aatttaatta atttgctact aaacta
//
LOCUS       DACXMZ010000426          872 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.427, whole genome shotgun sequence.
ACCESSION   DACXMZ010000426 DACXMZ010000000
VERSION     DACXMZ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.427"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(65..775)
                     /locus_tag="JD016_19660"
     CDS             complement(65..775)
                     /locus_tag="JD016_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5001803.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTLDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT          283 a          123 c          106 g          360 t
ORIGIN      
        1 ctaaaaacta agctttaaaa aattaagttt caaaaataaa attctagaga ttgaacttca
       61 aaaattaaga ttctaacata taccttgtaa attttcctac aacctcttga tattttttac
      121 ggcttatagg aattttttca ttctgaactt tggcataagt ttgagttata ctatctacat
      181 ggtttagatt gacaatatac ctttgatgcg tacgtataaa attagaggga agaacagttt
      241 ccatatcatt taattttccg tagtattcta atgttttatc aagggtaact acagttaatt
      301 ttcttagctc acttttaaag tagaggatat cttttgtatt aattttacaa agattagttc
      361 caatctgaac tgtaataaag ttttctttcc attgttctaa tttttttagt gcttctttta
      421 atactttaca tattttttca gttttataag gttttaagat gtagttcaaa gctcctacat
      481 catatccatg aaagacatag tcagaaaaac ctgtgacaaa gatgattata gtatcttttg
      541 aatgttttcg tatagttttt gctatgtcaa cgccattact ttttccaagt tcaatatcta
      601 aaaaaataat atctatgtaa taattttcgt gttctagatt gtatactaga ctttctccat
      661 ttgaatattc ttttatttgg taggggtagc cccacaaatc catttctctt gtaatgattt
      721 tattcagcat gtttctttgt tgtttttcat catcacaaat acaaatcgaa gtcaattttt
      781 tatctccttt caagttttat atttatatag ttaagtatat aatatatttt gctacctaga
      841 tatataaaat gaaataacaa ttatatccag at
//
LOCUS       DACXMZ010000427          853 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.428, whole genome shotgun sequence.
ACCESSION   DACXMZ010000427 DACXMZ010000000
VERSION     DACXMZ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.428"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            39..>853
                     /locus_tag="JD016_19665"
     CDS             39..>853
                     /locus_tag="JD016_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAU5001804.1"
                     /translation="MLNIYISETDLIKAQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAVNNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGIEWLHPYIDRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFVQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTL"
BASE COUNT          352 a           80 c          157 g          264 t
ORIGIN      
        1 cttaaaaata atggaataag cttcttaaag taaaggaggt gttaaatatt tatataagtg
       61 aaacagattt aataaaagct cagttaaaaa aagagagtac ctttaaccta gtaaaagtaa
      121 tagaacacta catcttaaag catagaccag agaaatacaa acaaggagaa gaatactatt
      181 atggtaatac tgatgtaaac aataagagaa gatattatct cttagatgga gctaaggttg
      241 atgattttac taaggttaat aataaagcag ttaataatta tcataagctt ttagttgacc
      301 agaaggtggg atatagcgtt ggaaatccga tagtatttaa tgcagatgat gataatctca
      361 ctaagctttt aaatgactta ctgggagaag aatttgacga tacaataaca gagttatatc
      421 ttaatgctag taataaaggt attgaatggc tacatccata tattgataga aaaggtgagt
      481 ttaaatatgt gataatacca gctgaagaag caattcctat ttgggatagt aaaagacaga
      541 gggaattagt tgcatttatt aggttttatt atattgagga tatagatgga aacaagataa
      601 aaagagttga atattataca gaaaatgatg taacttactt tattgaaaga ggtaatagtt
      661 ttgttcaaga atttttatat gatgaatatg gaaaaatgac tgatatacaa gaaggtcatt
      721 ttagaataaa taataaagag caaggatggg gcaaagtacc gtttatacct tttaaaaata
      781 atgaaaaatg tgtatcggat ttaactttct ataaatcatt gatagatata tatgacaata
      841 atatttctac tct
//
LOCUS       DACXMZ010000428          847 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.429, whole genome shotgun sequence.
ACCESSION   DACXMZ010000428 DACXMZ010000000
VERSION     DACXMZ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.429"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(100..789)
                     /locus_tag="JD016_19670"
                     /pseudo
     CDS             complement(100..789)
                     /locus_tag="JD016_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
BASE COUNT          300 a          213 c           83 g          251 t
ORIGIN      
        1 catcaaaaca ccttttcaat tctttattcc taattttatt accctctaat ctaaatatta
       61 tatgtctatg aaactgtcta aagctcaaga tttctagcat tattttcgac gtagcaacac
      121 tacagtctca caatgtggcg tatttgggaa catatccata cacttaacca tcttaatctc
      181 ataaccatac cccttaaaat caaccaaatc aacaaccaaa gacttaggat tacaagaaat
      241 ataaacaatc tccttagcac caaatccaca aatatcccta atagcatcct tatgaatgcc
      301 tggtctagga gggtcaacaa taatcaaatc aggcttgtcc tttaaatcct taacaacctt
      361 agccacatcc ccagcaataa actcacaatt agtaagtcca ttcaacttag cattctcatt
      421 agcagcaaca acagcttcct caactatctc aataccatac actttcttag cagcaccagc
      481 cataacctgt ccaatagacc cagtaccact atacaagtca aatacaactt tatcattgta
      541 atctccaata aaatccctag caatactata aagaacctct gctcccttag tattggtttg
      601 gaaaaacgaa aatggtgaaa tcttaaactt aagacctaaa atttcttctt gtatataatc
      661 tcttccatac aatactctta actcatcaca ttggactgca tctgctaaac catcattaat
      721 agtatgaagt acacctacta actcagcttt taaatcaatc cctaaaagca tatctttaaa
      781 ctcattcatc tgaacctccc atgactaaag tcacagggtt cctgcttcat agaattttgc
      841 atactaa
//
LOCUS       DACXMZ010000429          842 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.430, whole genome shotgun sequence.
ACCESSION   DACXMZ010000429 DACXMZ010000000
VERSION     DACXMZ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.430"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..644
                     /locus_tag="JD016_19675"
     CDS             <1..644
                     /locus_tag="JD016_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001805.1"
                     /translation="RFNLGLQNGEQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDS
                     YSNFIIYDIEDNTFNISNKFMDNLNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTD
                     MSNSITNLVYDINSNEIKLEDYEILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSI
                     SGELKGFKGIVDVVGTKPVKFQVFDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT          355 a           63 c          130 g          294 t
ORIGIN      
        1 atagatttaa tttaggatta cagaatggag aacaacaaaa tatcaatgaa attaaatttt
       61 ctattggaag taaattatac ttagaaatag taaataataa aagtaaaaat ggatatattg
      121 aaggacatga tagttactct aactttatta tatatgatat agaagataac acttttaata
      181 taagtaataa atttatggat aatctaaaca aagaaaagaa tgcaaatcca aaggagattg
      241 gcttattaag taaaactaag ctagattata tggtaaaaaa caataaattg aatcttatag
      301 ttacggatat gagtaattcc ataactaatc ttgtttatga tattaattct aatgagatta
      361 aattagaaga ttatgagata cttgatttaa aagtagatta taatgaagca tcatctggag
      421 ttaggaaagt taagctactg gatgataaga tatattctat ttcaggtatt aagaacagta
      481 tatcaggaga attaaaagga tttaaaggga ttgttgatgt agtcggaact aagccagtaa
      541 aatttcaagt atttgatatg aaacttaaaa agaatattta tgaagctgag ctaataaatg
      601 gagatataag tttacaagaa aagttatttt ttgttgcaaa ttaagtgtta gaattaattt
      661 tagaacttta aacttttata tgtatcacag ataagtataa gtaagggagt gtctcaaaat
      721 aaacttttta gctcatgaga cacttttttt atatgaaatc aaatatattt tcattgttta
      781 gatttatttt gataatgaaa aaaagattat ctttatttta agaaagacat ttatttataa
      841 tt
//
LOCUS       DACXMZ010000430          810 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.431, whole genome shotgun sequence.
ACCESSION   DACXMZ010000430 DACXMZ010000000
VERSION     DACXMZ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.431"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            557..>810
                     /locus_tag="JD016_19680"
     CDS             557..>810
                     /locus_tag="JD016_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5001806.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKIS"
BASE COUNT          271 a           93 c          112 g          334 t
ORIGIN      
        1 taatctttta tgacttattt atatttttca tttttataaa aaaacaataa ttaagaatat
       61 aactatattc tacatgtaaa aattttttta tttaaactca attttctgat aatttgataa
      121 tttttcaaaa tttatagttg acttttattt tttactgtat taaactaatt actatacaaa
      181 acagaaaaca aaattttcaa aattattaaa ttaaaaggag aggtaaatat gaagacttta
      241 ttaaacaaac aagtacgatt ttttagtagc caatttagtt agggtttgta cctaagttaa
      301 gacaaattgt cataacatag atacaagccc cttaggatta tgatttttaa ggattgtatc
      361 tatgttggct atagtaattg ttgttaaagt ttttatttta tcatcataat atagagccac
      421 atggattata ctgtgtggct tattcgaatt tttgccatac agttatatat actgtgtggt
      481 ttttttatat caaaatcatt ttttaaaacc aaatttaaaa aaccatttaa aatattttca
      541 aaggggtgtt ttatttatgt caggtattat atctgtaatg acgcaaagtt taattttatc
      601 gattatggca ctgggggttt atataactta caaaatctta gattttccag atatgtctgc
      661 tgatggtagc tatacaatgg gtgcttctat tgtagcattt tctcttacta atggtatttc
      721 tcctgtagta gcaactttaa tggctatctt atgtggttgt actgctggtc ttgttacagg
      781 gattcttcac attaagttta agatatctaa
//
LOCUS       DACXMZ010000431          801 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.432, whole genome shotgun sequence.
ACCESSION   DACXMZ010000431 DACXMZ010000000
VERSION     DACXMZ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.432"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            179..745
                     /locus_tag="JD016_19685"
     CDS             179..745
                     /locus_tag="JD016_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU5001807.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
BASE COUNT          292 a           99 c          123 g          287 t
ORIGIN      
        1 ttattttctt tatattaatt taacaataat tcattttcat agacttttat ttggatttaa
       61 ttaaatttta attgtcaaaa tctactatgt attcttattg ccactacttc tatgttgtgc
      121 tataattact ttgttttaag cgaatatttt atattaattt tattatttgg aggttcttat
      181 gctaggtgaa aagatgagaa atataagaaa aagcaagaaa aaaacattaa gtgatgtttc
      241 caaattgaca gatttatcta taagttatat ctctcaaata gaaagagatg ctattgaacc
      301 atccttgtct tcattgagaa aaatcgctga agttttagac actcctttat atatgtttat
      361 ggatgacaat aatactgatg atttagtcat tagaaaagaa gatagagtta tgatgaaatt
      421 tccaaagagt gaaatgtttt acgagatagt atctccaatg cctacaactg agtttacacc
      481 ttcaatttta tttgtagagt ttgaacttaa accagaaagt gaggatgcca aagattatat
      541 atatcatgca tcagaagaaa ttatggtggt aacgagtggt gtggttgata tttgtatggg
      601 tgaaaaggta gttagattaa atccaggtga ctcaacattt ataaaagcta atgtaccaca
      661 taaaatcatc aatccaagta aagatacaat agctcgtgga tatggagtta tatcacctcc
      721 aatttggcca ataaaatcta aataaactca atataacaca cctacttttt gtagataata
      781 taatagattt aaatattgct t
//
LOCUS       DACXMZ010000432          794 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.433, whole genome shotgun sequence.
ACCESSION   DACXMZ010000432 DACXMZ010000000
VERSION     DACXMZ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.433"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            111..488
                     /locus_tag="JD016_19690"
     CDS             111..488
                     /locus_tag="JD016_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAU5001808.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT          333 a           83 c          109 g          269 t
ORIGIN      
        1 aataacccta tggtaaaaat tggaaaatat tattatcgac aaaatctttg acttaaaaac
       61 tatattgcga tagtattaat ttaataaaag tttgagaggg gcgtaaggtt atgacaattt
      121 caaaaatacc acaagcagaa ttaaaagtta tgaagtttat ttgggaaaaa aatgatacag
      181 taacatcaaa agaaactatt gaagctatgg aacgaaaata tggttggaaa caaacaacta
      241 cattgacact tttatcaaga cttgttaaaa aacatttttt aagtgcagaa aaaatagata
      301 gatacactca ttatacaata gttgttggac ataaggagta tttaagtgtt gagacaaaag
      361 attttttaag caatatacac gacaattctt tacagagttt aatttcagca ctacatgatg
      421 atgaagttgt tgataaagat atgttggatt tatttgaaag tcattttaaa aatttagaag
      481 aagagtaatt atatcaggat agaactctaa gaatttaatt aatctatggc tgaaaaccat
      541 aaatggtatt atttaatcag ctactcacta ttcataaagt aatattaaat aggttctcta
      601 attagtaaaa tgcctaaaat aattggaata gggagtatca agttaaatga taccacttat
      661 ttaaataaga aatgattttt aaaatttaaa ataattttat aacttgtcaa tattcattaa
      721 tctaaagtat tatttctatt taaccatatc aaaatatatt ttactatttt taattattac
      781 aatataaacc tgtc
//
LOCUS       DACXMZ010000433          787 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.434, whole genome shotgun sequence.
ACCESSION   DACXMZ010000433 DACXMZ010000000
VERSION     DACXMZ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.434"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            261..>787
                     /locus_tag="JD016_19695"
     CDS             261..>787
                     /locus_tag="JD016_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001809.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYK"
BASE COUNT          353 a           60 c          103 g          271 t
ORIGIN      
        1 ataaatatga gaacttaaat tctcttatct atgtgaaaat aaacatctct taaaatgaaa
       61 ttctgtaaat ataaatgatt ttttagtttg aaggattaga ttaacaaaat tataaatttg
      121 caaataagct atattaaaaa atataataat tttttatata acttattctt ttataacttt
      181 taattttaaa agaggagaat gtatataaga gtattcaatg tactagaaaa aatcaaaaga
      241 aatattagaa gaaatggata ttgagtaatc aaagaaaaga ttttgatatt acaaatgata
      301 tgtttcaaga aagcattcct atcacaacaa aaattattct tgaagacatg ccttcaaatg
      361 atgaattaaa tcatgttttt tcaaaaggtt gtgaacgaaa aatgaaaaag agaaaaattg
      421 tattaatcac actactatta ataggtgttt tgttattagg aagtatccta tataatcttt
      481 ttttaggaaa aacagctaat atttcaatgc taaaggaatc ttggaatttt gatataccaa
      541 tacctaacaa agaaattgaa gtatttgata ctcaagatag tattaatgga gatggacaaa
      601 gttattttat tcaaggattt tctgaaaaaa atttcaaaaa agtttttaat ttaaaaggtg
      661 gtatagttgt aagcaaagat aatattaatg aaatagaaaa atatattgat aaatttaaaa
      721 gagattctgt taacattaat aaaagtaata aaaataaaat tgaagaagat tttaaaaaat
      781 ataagtt
//
LOCUS       DACXMZ010000434          776 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.435, whole genome shotgun sequence.
ACCESSION   DACXMZ010000434 DACXMZ010000000
VERSION     DACXMZ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.435"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            79..>776
                     /locus_tag="JD016_19700"
     CDS             79..>776
                     /locus_tag="JD016_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAU5001810.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTILENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          288 a          108 c          128 g          252 t
ORIGIN      
        1 aaattctata ttacaacatt attttatagt ttgattaatt taacaatcac taatatatca
       61 aactgaaagg agatttttat ggaaaaaaaa tatttcgaaa ttcatggagt tccttcaatc
      121 ctttggggaa atccttcaag taaattatat atttacatac atggacaatg tggcaataaa
      181 gaggaggctg aagcttttgc taatattgca gttccacatg agtgtcaagt tttaagtgtt
      241 gatttgcctg aacatggaga acgtaaaaga gaaaaaaatt catttaaccc atggcatgtt
      301 gttccagaat tagtatctat tatggaatat gcaaagtgcc actggaatca aatttcgtta
      361 tatgcaaata gcattggagc atggtttagt atgttaagct ttgaaaaaga gaacttaaga
      421 gagtgtattt ttgtatctcc aattctagat atgcaacagc tcatttcaaa tatgatgctt
      481 tgggcaaatg tatcagaaga acagcttaaa catgaactaa ttatcccaac ttcatttggg
      541 catactcttt catgggaata tctacaatat gtaaaacaac atcctatcac tcaatggaat
      601 gtaccaacaa aaatattata tggtaaacat gatgaactta ctgaaatcgg tgttgtagag
      661 aaatttgtag aaaagtttaa ttgcaacctt actattctgg aaaatggcaa acattggttt
      721 aacacaccac aagaattaaa ttcattatat gaatggttaa atactttagt taaata
//
LOCUS       DACXMZ010000435          773 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.436, whole genome shotgun sequence.
ACCESSION   DACXMZ010000435 DACXMZ010000000
VERSION     DACXMZ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.436"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            81..548
                     /locus_tag="JD016_19705"
     CDS             81..548
                     /locus_tag="JD016_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAU5001811.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          282 a           45 c          150 g          296 t
ORIGIN      
        1 tactagacct tattgaaacc tttatagttt aattctaatt atatattata aataaagtat
       61 gtgtatatta aggaggtaat atgaagtttt taaatgtttt aatagtagtt gaagatattg
      121 agaaatcaaa aaagttttat tatgatgttt tggggttaaa ggtaatctgt gattttggag
      181 aaaatgttgt attagaaggt aatatatcat tacaagaaaa aaagctttgg ttagaattta
      241 ttaataagag tgacagtgaa gttaagttca atggaaatga tgctgagtta tattttgagg
      301 aagataattt tgatactttt gttgaaagat tgagtacaat gaaagatata gattatgttc
      361 atctagctat agagcatagg tggggtcaaa gagcaattag attttatgat ttagatgggc
      421 atattattga ggttggagaa actatgtcat ctgtgtgtag acgtttttta gatagtggtc
      481 tttcaataga tgaggtagct aaaagaatgg atgtaactgt cgaatatatt gaatcagttt
      541 tagaatagat ggattatatt aaagtaaaac tatattttgt atagaagtaa gggctgtaag
      601 taaacttgta tggaaacaaa tttacttaca gtttttgttt ttgtaagata cagaatataa
      661 tatttgttat aattttattt ggatttttaa aaagtgcatg attttcctta catattataa
      721 tagaatttaa tatttattgg atgatttaaa tttagaaaaa tataagtatt agc
//
LOCUS       DACXMZ010000436          753 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.437, whole genome shotgun sequence.
ACCESSION   DACXMZ010000436 DACXMZ010000000
VERSION     DACXMZ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.437"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>753
                     /locus_tag="JD016_19710"
     rRNA            <1..>753
                     /locus_tag="JD016_19710"
                     /product="23S ribosomal RNA"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DACXMZ010000437          748 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.438, whole genome shotgun sequence.
ACCESSION   DACXMZ010000437 DACXMZ010000000
VERSION     DACXMZ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.438"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            169..675
                     /locus_tag="JD016_19715"
     CDS             169..675
                     /locus_tag="JD016_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAU5001812.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          300 a           75 c          132 g          241 t
ORIGIN      
        1 tacacttata aaatatgaaa actacttatt gcgaaagtaa ttgatttgtt gtattataat
       61 ttacatgaat tatgtaggac aatcaaaagt cagtattata agagtacata tattcaaatt
      121 tttaacaatt aatttatata attaaaaaat gaagggtagg atgaagtcat gggttttaga
      181 gaagtaaaaa ttgaggaatt acagtttaat ccatttacta agattggcaa agaatggtta
      241 cttataacag ctggtgattc agaaaagttt aacacaatga ctgctagctg gggtggtgtt
      301 ggagtctatt ggggtaaaaa tgttgttaca acttacataa gaccacagag atatacaaaa
      361 gaatttgtag atagtaatga tacatttaca gttgccttct ttgatgaaac gtatagagag
      421 gctttaaata tttgtggtac tatttctggt agagatataa ataaaataga aaaagcagga
      481 cttacaccat attttgtgga tgatacagta gcttttgaag aagctaatat gattatagta
      541 tgtaaaaagc tatatcatgg taatatgcca ccagaaaact ttgatgctaa agaaaatgat
      601 aaaaaatggt atccagagaa agactaccat actatgtaca tttcagaaat tataaaagtt
      661 cttgtaaaag aataattata ataaaaaata tatgaaaaga acttattata agtaaatgac
      721 tgtgctttct tatctgatat ctaagtag
//
LOCUS       DACXMZ010000438          744 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.439, whole genome shotgun sequence.
ACCESSION   DACXMZ010000438 DACXMZ010000000
VERSION     DACXMZ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.439"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            48..>744
                     /locus_tag="JD016_19720"
     CDS             48..>744
                     /locus_tag="JD016_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAU5001813.1"
                     /translation="MSEKRRDNKNRILRTGESQRKDGRYAYKYIDTFGKPQFVYSWKL
                     VPTDKTPAGKRDDIALREKEKEIQKDLDDGIDHIGKKMTVCQLYAKQIRHRANVRHGT
                     KQGRKQLMRILQEDKLGACRIENVKLSDAKEWALRMKEKGYGFKTINNHKRSLKAAFY
                     TAIQDDCIRKNPFDFQLNTVLEDDTEPKEPLSPTQEAAFLSFVQHDKVYQKYYDEIII
                     LLGTGLRISELCGL"
BASE COUNT          250 a          158 c          180 g          156 t
ORIGIN      
        1 tagtcaagca tatcaaggct ctttccatta tggaaaggag cataacgatg tcggaaaaaa
       61 gacgggataa taaaaatcgt attctccgca ccggagagag ccagagaaaa gacgggagat
      121 atgcctacaa atatatagat acctttggaa aaccgcagtt tgtgtattct tggaagttag
      181 tacctacgga caaaaccccg gcaggaaaac gcgacgatat agcgttgagg gaaaaagaga
      241 aagaaatcca aaaagacctt gacgacggta tcgaccatat cggaaagaaa atgacggtct
      301 gccaactcta cgcaaagcag atacgccacc gggcaaatgt gcggcatggt acaaagcagg
      361 ggcgaaaaca gctcatgcgg attttgcagg aggataaact tggagcctgc cggattgaaa
      421 atgtgaagct ctccgacgca aaggaatggg cgttacgcat gaaagaaaaa ggttacggct
      481 tcaagactat caacaaccac aagcgttcct taaaggctgc cttttacaca gccatacagg
      541 acgattgtat tcgtaaaaat ccatttgact tccagttgaa cacagtcctt gaagatgata
      601 cggaaccaaa ggaacctcta tcccctacgc aggaagcggc ttttctgtcc tttgtgcagc
      661 atgataaagt ctatcagaaa tactacgacg agattatcat actgttaggg acagggcttc
      721 gcatttcgga actctgcgga ctga
//
LOCUS       DACXMZ010000439          738 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.440, whole genome shotgun sequence.
ACCESSION   DACXMZ010000439 DACXMZ010000000
VERSION     DACXMZ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.440"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     regulatory      3..96
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            179..664
                     /locus_tag="JD016_19725"
     CDS             179..664
                     /locus_tag="JD016_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAU5001814.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          322 a           64 c          122 g          230 t
ORIGIN      
        1 tattcagata taggcaatat tagataaatc taatgacgca aaactatagg gactaaaatc
       61 tttatgtaat aaaggtcatg tcagccagtt gccaaaaaga taataattct ttttgttaaa
      121 agtattttta gagttatata ttttaataac ttaatatttg gcaagaggag ggcttaagat
      181 gatatctgaa ttaatgttaa aaaattttcg caaatctagc atcaatttta agactaaaat
      241 taaattgaat ttaagagaaa aaccagacat aaatagtttg aaattaaaaa gtataccaga
      301 aggtaaaatt gttaagttaa agtgtgtaga tggaatatgg gcagaagttg agagtaatta
      361 tgacaaaggt tggttacttt ataaatattt agaaagatta agtaatgcta aaaacaatag
      421 taatgataca ataaaaaata gaaaaaagaa ttacatagga aatataagaa caaatggatt
      481 aagtttagaa cttagaaatg atagaacatt agaatctaaa gttataacta caatccctga
      541 tggttttaaa gttgaagtat gttatagcgt tggaaaatgg gcaagagtta atatcaataa
      601 aaatgaaaaa cgatattctg gatatgtata taatcaatac atagaagagc atttaagttt
      661 ataatacgat actagcaagc tttaaatata ttaataaatc gcaaaaaaga atcccattaa
      721 tgggattctt ttttgtag
//
LOCUS       DACXMZ010000440          727 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.441, whole genome shotgun sequence.
ACCESSION   DACXMZ010000440 DACXMZ010000000
VERSION     DACXMZ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.441"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(9..200)
                     /locus_tag="JD016_19730"
     CDS             complement(9..200)
                     /locus_tag="JD016_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001815.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            505..711
                     /locus_tag="JD016_19735"
     CDS             505..711
                     /locus_tag="JD016_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAU5001816.1"
                     /translation="MFKKKGGYTFTNFLSKVRVEKSKKYILKKNLSILDVAVLIGFNS
                     QNYYSIIFKKFNNFTPIEYKNIYN"
BASE COUNT          281 a           91 c           62 g          293 t
ORIGIN      
        1 ttttatgttt atctatgttt taaatacaca aaatattctt ctacttcatc tccatttttt
       61 acttttacta aatattcatt ataatgagca ttatatggct gtgtcatttt gcattctatt
      121 acattttcta tgtcttctaa ttctaaaagt tctacatcct ttaaaaattt tctttctatg
      181 agttccatta atctttccat aatatatcct cacttcccct ataattaaga taaactatat
      241 atcctcttaa tacagagttt atctgtttat tatattatat ccaatatatt acagtttcta
      301 acatcattca taaattattt taacaaatag agattgattt tttaataaat atgccttgat
      361 aaaatgaaac atagaccaac tcttagtttt atgtaaagaa aacttaaaga aaactataga
      421 ttatattcac aaaaattact cagaagatat aacagtaagt aatatatgta atgaattaca
      481 acttaataaa agttgttttt ttctttgttt aagaaaaaag gtggatatac ttttactaat
      541 tttttaagta aagttagagt tgaaaaaagc aaaaagtata tattaaaaaa aaacttgtct
      601 attttagacg ttgctgtact aattggattc aatagtcaaa attactatag tataattttt
      661 aaaaagttta ataactttac accaattgaa tataaaaata tttataacta aaaaatatca
      721 ttctaaa
//
LOCUS       DACXMZ010000441          722 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.442, whole genome shotgun sequence.
ACCESSION   DACXMZ010000441 DACXMZ010000000
VERSION     DACXMZ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.442"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="JD016_19740"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="JD016_19740"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..365
                     /locus_tag="JD016_19745"
     tRNA            291..365
                     /locus_tag="JD016_19745"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
     gene            372..457
                     /locus_tag="JD016_19750"
     tRNA            372..457
                     /locus_tag="JD016_19750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:406..408,aa:Leu,seq:taa)
     gene            473..548
                     /locus_tag="JD016_19755"
     tRNA            473..548
                     /locus_tag="JD016_19755"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:506..508,aa:Met,seq:cat)
     gene            556..630
                     /locus_tag="JD016_19760"
     tRNA            556..630
                     /locus_tag="JD016_19760"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:589..591,aa:Glu,seq:ttc)
     gene            640..713
                     /locus_tag="JD016_19765"
     tRNA            640..713
                     /locus_tag="JD016_19765"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:672..674,aa:Gly,seq:tcc)
BASE COUNT          208 a          155 c          171 g          188 t
ORIGIN      
        1 attcattaag atatatgtgt agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agccaaaaaa agccgaagtg gcggaactgg cagacgcaca ggacttaaaa tcctgcggga
      421 cttacctctc gtaccggttc gattccggtc ttcggcactt ataaaacaat atcgcggagt
      481 ggagcagttg gcagctcgtc gggctcataa cccgaaggtc gtaggttcaa gtcctgcctc
      541 cgcaaccatt aaaatggctc gttggtcaag aggttaagac accgcccttt cacggcggta
      601 acaggggttc aattccccta cgagtcacca attaaatatg cgggtgtagc tcaatggtag
      661 agttctggcc ttccaagcca gctgtgaggg ttcgatcccc ttcacccgct ccaaaaatgg
      721 ga
//
LOCUS       DACXMZ010000442          705 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.443, whole genome shotgun sequence.
ACCESSION   DACXMZ010000442 DACXMZ010000000
VERSION     DACXMZ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.443"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(14..>705)
                     /locus_tag="JD016_19770"
     CDS             complement(14..>705)
                     /locus_tag="JD016_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAU5001817.1"
                     /translation="GKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLLKKYPNNVD
                     IIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRYLEKPLKYS
                     LFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHTEKGIYTFK
                     SSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSRYKIKLLRE
                     KLNSCLVDVII"
BASE COUNT          242 a          102 c           50 g          311 t
ORIGIN      
        1 gcaaaaattt acctcaaatt attacatcta ccaaacaact atttaacttt tctctcaata
       61 attttatctt ataacgactt acaattattg aattttgatt tataaataca atattatttt
      121 ctatatctag catatttacc ttcattaaat ttataaggta gcttttatga catctaaaaa
      181 aattatgttc ttctagattt gattctattt ctttcatact actcttaaaa gtatatattc
      241 ctttttcagt atgtatagtt aaatcatttt ttaaaacttc taaatataaa atatcatcta
      301 cctttatgaa atatatcttg catttacatt ttatagataa aatagcttca tttttgtagc
      361 attcttttaa atatgatgat attgcttctt taaatagaga gtattttaag ggcttctcta
      421 aatatctata aactcctatc tcataacctt tctttatgta tttatttgat gaggatacaa
      481 atataattct tacactttca tcaaattttt tgatttcttt tgaaacatta aatccatttt
      541 catcatttaa gtctatatcc ataaatatga tatcaacatt attaggatac ttttttaata
      601 aatcgcctcc attttcaaac tctttgatat tgtattcaaa attgtaatta taaaaaaagg
      661 tatttatata atcatttaga attaccctct ctgatttttt acctt
//
LOCUS       DACXMZ010000443          660 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.444, whole genome shotgun sequence.
ACCESSION   DACXMZ010000443 DACXMZ010000000
VERSION     DACXMZ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.444"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            70..>660
                     /locus_tag="JD016_19775"
     CDS             70..>660
                     /locus_tag="JD016_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAU5001818.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT          265 a           68 c          145 g          182 t
ORIGIN      
        1 ataaattaaa aaagtgaaat atcaaaataa aacgtcaaca aattaaaaat tatatggggg
       61 aatatcaaaa tgttaaatga aacaagaaaa aaacaattaa ctgaagtatg ccaagactta
      121 ataagaaatg ctagttattc aggtcaagaa gaaaacgtag taaaagctat agaagaaaat
      181 tttgctttac taggttttga tagttggtca agagatagat atggaaatat agtaggatgt
      241 ataaaaggta acaagccagg taagaaaata ttatttgacg gtcatataga tacagttcca
      301 gttccagatg caagtaaatg gagtgttcct ccttttgaag gaaaaatagt tgatggaaag
      361 atatatggta gaggaacttc tgatatgaag gggcaagtaa gtgctatgat ggctgctgtt
      421 tcatactttg cagaagatac taacaaagac tttgaaggtg aattgtatgt agcaggtgtt
      481 gttcatgaag aaatatttga aggtgtttca gctagagaga taagtaaggc tgttcaacct
      541 gattatgttg taataggtga atcttcagaa ttaaacttaa agataggtca aagaggtaga
      601 ggagaaatag tagtagaaac ttttggtaaa ccagctcact ctgctaatcc agaaaaaggt
//
LOCUS       DACXMZ010000444          654 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.445, whole genome shotgun sequence.
ACCESSION   DACXMZ010000444 DACXMZ010000000
VERSION     DACXMZ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.445"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(78..269)
                     /locus_tag="JD016_19780"
     CDS             complement(78..269)
                     /locus_tag="JD016_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HAU5001819.1"
                     /translation="MAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQ
                     SAGTAATLTIFRIADTVMT"
     gene            complement(525..654)
                     /locus_tag="JD016_19785"
                     /pseudo
     CDS             complement(525..654)
                     /locus_tag="JD016_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089747.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
BASE COUNT          177 a          137 c          115 g          225 t
ORIGIN      
        1 gtaaaactca atatataaaa atacttttgt catttaaata aagcctaaat agtttttaag
       61 attattttag acacgtttta agtcataaca gtatcagcta ttctaaatat cgtcaaagta
      121 gccgcagttc ctgctgattg cccatttcgt atccctactg ttgtagatga aaaattattt
      181 attgtaaaag tagtcccacc aaataaaaaa ccagcaaaat atccagaaag tactcctccg
      241 cctcctggct cagtagtaag ccctgccaaa tcttttcctc ctaaaattgg gaataggata
      301 gcgaaagaat attgatttat acttgatgaa cctggttgta atgctactga aactgatata
      361 taatacacac cattttcttg tactgtaaac gtaccatttg catctactgt tatacttgaa
      421 ctatttccta ttattgtttg ccaacctgtt atagcatctc cattatttag tgtttgtgga
      481 gatggtgcat ttacgaggaa gttagcaact ggctgagagg aattgcctgt tgctcctgtt
      541 ggacctgttg gacctgttgg gcctgttact cctgtcgctc ctgttgctcc tgttggacct
      601 gttactcccg ttggccctat acttcctgtc gctcctgtat ttcctgttgg gcct
//
LOCUS       DACXMZ010000445          648 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.446, whole genome shotgun sequence.
ACCESSION   DACXMZ010000445 DACXMZ010000000
VERSION     DACXMZ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.446"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            231..599
                     /locus_tag="JD016_19790"
     CDS             231..599
                     /locus_tag="JD016_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAU5001820.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT          283 a           56 c          112 g          197 t
ORIGIN      
        1 agtcacagaa aatagagtta atttaatata taataaaagc atatcaagta aagctttaaa
       61 ttaaagtaac tttatataaa aagtaatata aattgataaa attataaatt tatttatagt
      121 gaatatgggg tggattttat agatacaaaa atagtatata tgatatgtag accattagca
      181 tgtttaaagt agcaaaacat ataaaataaa atttgaaagg aagtataaaa atgacaagac
      241 catcaatata tcatagccaa ggatacactt gtgcagaggc attaataaag tcttataatg
      301 aggaacacaa cacagatata ccaatctcaa taggaagtgg tatgggggtt ggaatgaatg
      361 ttggaagtgt ttgtggagct gtaaatgctg ctgttgttat aatagggtat ttaaatgggc
      421 gaaatagtaa tttagatgaa aatatagcaa ggaagtattc aagagagcta ttgaatagag
      481 taagaaataa atacagctca gaaatatgtg tatctcttaa aaaaaataaa gtaacttgtg
      541 aagaaataat aaatttcaca tatgatgctc taaatgatat gctaaaaaat caaaaataat
      601 tctaagaaac aagtatttta cattcgatag aatacttgtt tttttatg
//
LOCUS       DACXMZ010000446          644 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.447, whole genome shotgun sequence.
ACCESSION   DACXMZ010000446 DACXMZ010000000
VERSION     DACXMZ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.447"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            40..>644
                     /locus_tag="JD016_19795"
     CDS             40..>644
                     /locus_tag="JD016_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAU5001821.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFFFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNIS"
BASE COUNT          226 a           56 c           85 g          277 t
ORIGIN      
        1 tataaaaaat ataatttaat catttttgag gatattatta tgttagatgc atttataaca
       61 attgtttctt ctattataga aatatggtca tgtaaaaaag tttttgatta tactagtcag
      121 tcaaagaccg acttaaaaaa actaaatatg tatttcttct tttctgtttt tctcattatt
      181 tcttctttat atataaatat aggagctaat agtagaatct tgatatgtat atttgtaatg
      241 ttcgttttct ataagtctaa ctataaggtt aatatatata aatgtctggt aatctcattt
      301 ttttattggt taatattaat aggtataaat ggattaagta tgttattagt agcaacagta
      361 agcaatttgg ataatatgaa tgttttactt agtggcagtt tatatagata tgaagcaata
      421 ttcttgggaa agataatgtt aggtatatta ctttatgttt ataaggcttc ttttaaaaat
      481 tttaaaataa gtaaaaaaga gatatacata tgtattccta tgatagcaaa tttattgtca
      541 ttttttgtac tttatgatta tattattaat cttgtaaaaa tctctcctga aagaagaact
      601 gcttttttag taatagtttt actattaata ttgtctaaca tttc
//
LOCUS       DACXMZ010000447          630 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.448, whole genome shotgun sequence.
ACCESSION   DACXMZ010000447 DACXMZ010000000
VERSION     DACXMZ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.448"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(82..564)
                     /locus_tag="JD016_19800"
     CDS             complement(82..564)
                     /locus_tag="JD016_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAU5001822.1"
                     /translation="MMSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEAT
                     LQGTIYGILMFILGVSYFIIIKSLIEVLGSSEHSLFVKENVKRFRTIGYLLLLNSLIE
                     FISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI
                     "
BASE COUNT          253 a          104 c           49 g          224 t
ORIGIN      
        1 tattataccc taatatattt taaattacta gttaatcact cttttcgggt tatttatctc
       61 ctatatatta aagtgtcaca cctaaatagt taaatcatta tcctctttta tctttattgc
      121 ctttacaaat ccatctgcaa ttacaaaact cataagagaa gttataaaat atacaaatac
      181 tggaacagta aaataaaatc caaatcctaa atctagaaat ctcattcctt ttccagtagt
      241 accaaaagtt gaaataaact caattagaga gtttaaaagc aaaagatatc caatagtcct
      301 aaatcttttt acattctctt ttacaaataa gctatgttca gaactaccta atacttctat
      361 caatgatttt ataatgataa aatatgatac acctaaaata aacattaata ttccataaat
      421 agtaccttgt agagttgctt ctccactaaa acagaggtac ataaaacttt taaatcctcc
      481 tgcaacaatt actaatccta aaagaaaaaa taaaatttgt aatataccac ttagtaaatt
      541 ttttataagt ttattagaca tcatgccaac tcctttaaaa tattttatat atagtataat
      601 acattatatt atatcgtttt tcaataaaaa
//
LOCUS       DACXMZ010000448          535 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.449, whole genome shotgun sequence.
ACCESSION   DACXMZ010000448 DACXMZ010000000
VERSION     DACXMZ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.449"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(17..>535)
                     /gene="bclA2"
                     /locus_tag="JD016_19805"
     CDS             complement(17..>535)
                     /gene="bclA2"
                     /locus_tag="JD016_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001089747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HAU5001823.1"
                     /translation="AIGPTGPTGANGATGATGATGATGANGATGPTGATGATGVLAAN
                     NAQFTVSSSSLVNNTLVTFNSSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLA
                     GFMSITTNFNGVTQNNFIAKAVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGT
                     SAAGYVSVYRIQ"
BASE COUNT          148 a          112 c           93 g          182 t
ORIGIN      
        1 cttttcatca tataaactat tgtattctat aaactgatac atatccagct gcagaagtac
       61 ctgtcgtgcc tgaaccaaat gtaaaactta aagtaacagc tgctgctcta gcatcaacta
      121 aaaagcttaa acttacactt acatctgatg aagtaagtgt atttactgct tttgcaataa
      181 agttattttg agttactcca ttaaagttag tagtaattga cataaatcct gcaagtgaaa
      241 gtgtggcacg tataccgaaa gatacattgt atatccctcc aactgcaaga tttatagtac
      301 tacttgttgg aaaagttata ttagttccat ttataaatga tgaattaaat gtcactaatg
      361 tattattcac taaacttgaa gaggacactg taaattgtgc attgtttgct gctaacactc
      421 ctgttgctcc tgtcgctccg gttggacctg ttgctccatt tgctcctgtt gctcctgtcg
      481 cccctgttgc tcctgtcgct ccatttgctc cggttgggcc tgttggtcct atcgc
//
LOCUS       DACXMZ010000449          519 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.450, whole genome shotgun sequence.
ACCESSION   DACXMZ010000449 DACXMZ010000000
VERSION     DACXMZ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.450"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(153..452)
                     /locus_tag="JD016_19810"
     CDS             complement(153..452)
                     /locus_tag="JD016_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_006589390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001824.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
BASE COUNT          195 a           70 c           53 g          201 t
ORIGIN      
        1 atattttata aactaatata ttttacaaat tgttgagtaa tattttatga tataaatcag
       61 aagaggcact tctataatag aaagtgctca gctatgaata agattaataa tatatttgcc
      121 ttagatatat agacttttat aaatattaaa gattaatcaa atctgatgtt tgaaacttta
      181 tcccaattaa tcttccttga atagtcttct ggatgtgata atctaactat atgagttgaa
      241 ttatcatagc tgtaataaat tgtagtagta ccatcaactg gattcttttt gtatccttga
      301 tacacagcac tatttggtac tttcttttca acaaagctat atgcagatga agtatcaata
      361 gtttctattt tattaggaaa tataaacata taaaaagcta ctactataaa aatacatatg
      421 catgtaatac tataccattt aatccatttc ataaaattcc tcctacttta ccaaaatata
      481 ctattattat ttccatatat ttataatatt tatacatac
//
LOCUS       DACXMZ010000450          467 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.451, whole genome shotgun sequence.
ACCESSION   DACXMZ010000450 DACXMZ010000000
VERSION     DACXMZ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.451"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            15..248
                     /locus_tag="JD016_19815"
     CDS             15..248
                     /locus_tag="JD016_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001825.1"
                     /translation="MKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLYL
                     EIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVD"
BASE COUNT          194 a           60 c           83 g          130 t
ORIGIN      
        1 ggaagtaatc tatgatgaaa atagcagtca taacaaccga atttttaaaa gagtttgtag
       61 ataattctat aaaaaagtta aatattaatg cagaaattga aatatatatc tatagagatt
      121 tttctcatgt tggagattta tatttagaaa tagaagatag gtttgatgga tttgcagtta
      181 gtggccccat ccccaaaaaa gctattacaa aaaaggctgg aactattaaa aaacctctag
      241 ttgattaaaa aatagctacg cacactcgtg actttagtca tgagttagta gctaaaatag
      301 tcagcatata gggaaaccta tatgtagaga taggatagaa cctatccaat actacttgaa
      361 ttgctggaaa cccctaaagc taaccaaact aaaacataag gatgaaataa acctaagtgt
      421 gaaagtcacg aaagtagaaa aaattggtta gatagtataa ggttaaa
//
LOCUS       DACXMZ010000451          438 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.452, whole genome shotgun sequence.
ACCESSION   DACXMZ010000451 DACXMZ010000000
VERSION     DACXMZ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.452"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            129..245
                     /gene="rrf"
                     /locus_tag="JD016_19820"
     rRNA            129..245
                     /gene="rrf"
                     /locus_tag="JD016_19820"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            251..339
                     /locus_tag="JD016_19825"
     tRNA            251..339
                     /locus_tag="JD016_19825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:285..287,aa:Leu,seq:taa)
     gene            351..427
                     /locus_tag="JD016_19830"
     tRNA            351..427
                     /locus_tag="JD016_19830"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:385..387,aa:Ile,seq:cat)
BASE COUNT          139 a           84 c          102 g          113 t
ORIGIN      
        1 aaacttatgg atttttaata gttttataag gcaaaaaatc gaataaaaaa agtattgaca
       61 caataagaca aagatgttat tataatactt gtcttagaaa aaaagttaaa tgaaaattta
      121 gacaataatc tggttataat agcagagagg atacacctgt tcccattccg aacacagaag
      181 ttaagctctc tagcgctgat ggtacttggt gggaaactgc ctgggagagt aggacatagc
      241 caggtgatgt gccgaagtgg cggaactggc agacgcacag gacttaaaat cctgcgggac
      301 ttacctctcg taccggttcg attccggtct tcggcaccac aaattattgt gggccattag
      361 ctcagttggt tagagcgccc ggctcataac cggtaggtct ggggttcgag tccctgatgg
      421 cccaccacaa taatttaa
//
LOCUS       DACXMZ010000452          422 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.453, whole genome shotgun sequence.
ACCESSION   DACXMZ010000452 DACXMZ010000000
VERSION     DACXMZ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.453"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>422
                     /locus_tag="JD016_19835"
     CDS             <1..>422
                     /locus_tag="JD016_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAU5001826.1"
                     /translation="QNELYIIEVNPRASRTVPYISKVSGVPIVDLATKCMLGAKLKDL
                     GYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVLGVGENLEEALYKGFL
                     AAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYT"
BASE COUNT          164 a           45 c           94 g          119 t
ORIGIN      
        1 caaaatgaat tatatataat tgaggttaac cctagagcta gtagaacagt tccatatata
       61 agtaaagtaa gtggagtacc aatagttgac ttggctacta agtgtatgtt aggagctaaa
      121 ttaaaggatt taggatatgg tacaggagtt tacaaagagc caaaattagt atcagtaaaa
      181 gtaccagtat tctcaatgtc taaattatct aaagttgaag taagtctagg acctgaaatg
      241 aagtctacag gagaagtcct aggtgtagga gaaaatttag aagaagcctt atataaaggg
      301 ttcttggcag caggtagaca tatgtctgat gaaagaggag tagtacttgc tactgtaaat
      361 aatcatgata aagatgagtt tatagagata gcaaaagata tgaaagaatt aggatatact
      421 tt
//
LOCUS       DACXMZ010000453          420 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.454, whole genome shotgun sequence.
ACCESSION   DACXMZ010000453 DACXMZ010000000
VERSION     DACXMZ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.454"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..68
                     /locus_tag="JD016_19840"
     CDS             <1..68
                     /locus_tag="JD016_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HAU5001827.1"
                     /translation="PKINDFGFNFTGVRPHENNNK"
     gene            80..>420
                     /locus_tag="JD016_19845"
     CDS             80..>420
                     /locus_tag="JD016_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HAU5001828.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQ"
BASE COUNT          179 a           58 c           73 g          110 t
ORIGIN      
        1 agcctaagat aaatgatttt ggttttaatt ttactggggt aagacctcat gaaaataata
       61 ataaatagga ggaaataaaa tggcagcact aaattatgca aaagaatatt caaatgtttt
      121 agcacaagca tatccttata ctttaaactt cggggatttg tatgcaacac caaataatgg
      181 aagatataga tggactggtt ctaaaacaat agaaatacca actatatcta caactggaag
      241 agtagattca aacagagata caatagcagt agctcaaaga aactatgata atgcttggga
      301 acctaaggta ttaactaatc aaaggaaatg gtcaacattg gttcatccag cagatataaa
      361 ccaaactaat tatgtggctt caataggcaa tataacaaaa gtatataatg aggaacaaaa
//
LOCUS       DACXMZ010000454          403 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.455, whole genome shotgun sequence.
ACCESSION   DACXMZ010000454 DACXMZ010000000
VERSION     DACXMZ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.455"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>403
                     /locus_tag="JD016_19850"
     CDS             <1..>403
                     /locus_tag="JD016_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAU5001829.1"
                     /translation="YFDNMRLSQDFGFNHANVVLRIGFKDGELYICNEIYVHEMDTSE
                     IIKIANSKGLEKNLFMYCDSAEPDRIKMWKSAGYRAKGVKKGPGSVKAQIDYLKQLRI
                     HVHPSCINTIKEIQQWKWKQDERTGLYLDEPV"
BASE COUNT          167 a           38 c           83 g          115 t
ORIGIN      
        1 tactttgata atatgaggtt atcacaagac tttggattta accatgcaaa tgtagtactt
       61 agaattggct ttaaggatgg agagttatat atatgtaatg aaatatatgt acatgagatg
      121 gatacatctg aaataataaa gattgcaaat agtaaaggtt tagaaaagaa tctatttatg
      181 tactgtgata gtgctgaacc agacagaatt aagatgtgga aaagtgcagg atatagagct
      241 aaaggtgtta aaaaaggacc aggtagtgtt aaagctcaaa tagattattt aaaacagtta
      301 agaatacatg tacatcctag ttgtataaat acaataaaag aaattcaaca atggaaatgg
      361 aaacaagatg aaagaacagg gttatatctt gatgaaccag ttg
//
LOCUS       DACXMZ010000455          402 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.456, whole genome shotgun sequence.
ACCESSION   DACXMZ010000455 DACXMZ010000000
VERSION     DACXMZ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.456"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
BASE COUNT          134 a           57 c           31 g          180 t
ORIGIN      
        1 tatgctaatt atcagcattt actaatttaa tcactattag ttatttcttt tattaaatat
       61 ctttactttt ttacttatta tgtcttctag ctttacattt ggattatggt atttctttct
      121 atttttagtc acatctttct attgaattac ttgcttaaaa caccaatgca cacatgccag
      181 ataatgttca caattatgta gaaatcttgc tctaccttct tcaatgttga tattatctct
      241 cctagttgat attatctttc ctaaaatttc ttttaattaa aaaacaacat tagttgatat
      301 atcaagtata ttttaagcaa ctgaaaaaaa caataataat tgatatatca actaattaat
      361 gtagattaat tcatttttat aatttatata ctttttaatt aa
//
LOCUS       DACXMZ010000456          399 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.457, whole genome shotgun sequence.
ACCESSION   DACXMZ010000456 DACXMZ010000000
VERSION     DACXMZ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.457"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            133..273
                     /locus_tag="JD016_19855"
     CDS             133..273
                     /locus_tag="JD016_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001830.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
BASE COUNT          182 a           23 c           68 g          126 t
ORIGIN      
        1 tgtagatata tcctaattat gtcaaaaaat aaaaaaattt ttatttatta gagtttttgg
       61 aaataataat aacagatatt ataattaatt aacagttttt gtaagtattt gacaatagag
      121 agggtgtaac aaatgggaat aatagagtca gcatcaaaat tggctgagat ggttcattta
      181 ttggctgttg aaaaggggat aactgatata gaagcatggg atgaagctgt gaaagaatac
      241 agcaaaattt atgaagaaag aagaaatgaa tagaatgata aaaaatgaaa acttgatgat
      301 aaaaaataac tttatttaaa gaaattcaat ttttatagaa gtattaaaaa taatataaat
      361 aaaaagatgg tatttcaaat gtaagtaaaa aatcatttt
//
LOCUS       DACXMZ010000457          393 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.458, whole genome shotgun sequence.
ACCESSION   DACXMZ010000457 DACXMZ010000000
VERSION     DACXMZ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.458"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            122..>393
                     /locus_tag="JD016_19860"
     CDS             122..>393
                     /locus_tag="JD016_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAU5001831.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFYQGTFKDVQFFLSKLELLKTDCKPFQFIV"
BASE COUNT          160 a           49 c           72 g          112 t
ORIGIN      
        1 aatacaaaca atataaatag cgatacagat gtggatggaa tagttaatgt cttaactgaa
       61 aaactgaacg atgctatggt tgtatcagcg gaaggaatag tttagaaagg agagtgagaa
      121 aatggcttat gatttttacc tagatggagt acaactacca atcgcaccac ccaagcttga
      181 agtcaaagtg acaaataaaa acaagacagt tgatttaata aatgttggag aagtaaatat
      241 attaaaaaaa gaaggactat ctgagataag ttttgaagca gaatttacac acaataagct
      301 accattttat caagggactt ttaaagatgt tcaattcttt ttaagtaaac tagaattact
      361 aaaaactgat tgtaagccat ttcaatttat tgt
//
LOCUS       DACXMZ010000458          384 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.459, whole genome shotgun sequence.
ACCESSION   DACXMZ010000458 DACXMZ010000000
VERSION     DACXMZ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.459"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>384)
                     /locus_tag="JD016_19865"
     CDS             complement(<1..>384)
                     /locus_tag="JD016_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAU5001832.1"
                     /translation="FVEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDTELL
                     ESSLVYWTTGAIAGCDINKSNTNKKYDGEFDVDVNYTQIQLEEALKSGKFIFHKVGDE
                     VHVLEDINTFVSFTDEKNDDFSSNQS"
BASE COUNT          120 a           82 c           33 g          149 t
ORIGIN      
        1 actttgatta gacgaaaaat cgtcattctt ctcgtctgta aaacttacaa aagtatttat
       61 gtcctctaac acatgaactt catcaccaac cttatgaaat ataaatttac cactctttag
      121 tgcttcttca agttgtattt gagtgtaatt tacatcaaca tcaaactcac catcatactt
      181 tttattagtg ttagatttat ttatatcaca tcctgctatt gctccagtag tccaataaac
      241 taaactagat tctaataatt cagtatcttt aatcttattt tctacagaca ctacaccttc
      301 ataatctgca tcacttttct tatatagtac tgtttgaaac ttagctccta ccttatctct
      361 cattctcttt gtaaattcta caaa
//
LOCUS       DACXMZ010000459          350 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.460, whole genome shotgun sequence.
ACCESSION   DACXMZ010000459 DACXMZ010000000
VERSION     DACXMZ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.460"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(39..332)
                     /locus_tag="JD016_19870"
     CDS             complement(39..332)
                     /locus_tag="JD016_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001086633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001833.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
BASE COUNT          133 a           74 c           18 g          125 t
ORIGIN      
        1 aaacatatat caaaatcaaa atattattaa tttatacttt aaagattccc atctttatca
       61 tattttattc ttataagaaa atataatcct attactacta actctatcac taaaaatact
      121 ccataggtaa gaattgaatc aatactatct aacccatttc taaaaaaaat taatgctata
      181 cctgctgcta taatccctat agcatttttt cttactgcat tttgcccaac tccataaaaa
      241 cctggcattt ttccttttat ttcgacagca gttaaaatta tcccaaacac tataaataat
      301 actccaataa gcatcataat catactaatc atctaattac ctccttcact
//
LOCUS       DACXMZ010000460          342 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.461, whole genome shotgun sequence.
ACCESSION   DACXMZ010000460 DACXMZ010000000
VERSION     DACXMZ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.461"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>342
                     /locus_tag="JD016_19875"
     CDS             <1..>342
                     /locus_tag="JD016_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAU5001834.1"
                     /translation="KFQTVLYKKNDADYEGVVSVENKIKDTGLLESSLIYWTTGAIAG
                     CDINKSNTNKRYDGEFDVDVNYTQIHLEEALKTGKFIFHKVGDEVHVLEDINTFVSFT
                     DEKNDDFSSNQS"
BASE COUNT          133 a           31 c           66 g          112 t
ORIGIN      
        1 aagtttcaaa cagtattata taaaaagaat gatgcagatt atgaaggtgt agtatctgtt
       61 gaaaataaga ttaaagatac tggattatta gaatctagct taatttactg gactactgga
      121 gctatagcag gatgcgatat aaataaatct aatactaaca agcgatatga tggtgaattt
      181 gatgtagatg ttaattacac tcaaatacat ttagaagaag ctttaaaaac tggtaagttt
      241 atatttcata aggttggaga tgaagttcat gtgttagagg atataaatac ttttgttagt
      301 tttacagatg aaaagaatga cgacttttca agtaatcaaa gt
//
LOCUS       DACXMZ010000461          321 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.462, whole genome shotgun sequence.
ACCESSION   DACXMZ010000461 DACXMZ010000000
VERSION     DACXMZ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.462"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
BASE COUNT          141 a           37 c           24 g          119 t
ORIGIN      
        1 ctatgattaa gcatcaattt tattttatac tttttgattt taatactata catctaaatc
       61 ttatttaggg ggaacaatta tttatatgat ataaaaatgc taatttgaat gaacttgtct
      121 tataatcagt tttagaaaat acaccattct acaaagtatg aatcaaaagg tgaaaaaata
      181 ctaaaaataa taaatcaaca tcaattttaa tcaatatatg aaaaacttat accaagtatg
      241 cataaaaact aatataaata ttattttaac aactaatact tacaccttta tatattatat
      301 catatttcaa taaataaatt g
//
LOCUS       DACXMZ010000462          314 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.463, whole genome shotgun sequence.
ACCESSION   DACXMZ010000462 DACXMZ010000000
VERSION     DACXMZ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.463"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..227
                     /locus_tag="JD016_19880"
     CDS             <1..227
                     /locus_tag="JD016_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAU5001835.1"
                     /translation="KDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLAT
                     GNLNISLKKNKCKASTKNKSEKRGVVDVTN"
     gene            214..>314
                     /locus_tag="JD016_19885"
     CDS             214..>314
                     /locus_tag="JD016_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAU5001836.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGE"
BASE COUNT          125 a           47 c           41 g          101 t
ORIGIN      
        1 tcaaagatac tactgcaata attgttggct cttttatata ccaattcact atatactttg
       61 caatgagttt aggaatgtta tcaactgact taaaactaat aactgccata gttataatag
      121 ctttcctagc tactggtaac ttaaatattt cactaaaaaa aaacaaatgc aaagctagta
      181 ccaaaaataa atcagaaaaa agaggtgttg tagatgttac aaattaaaaa tctttcaaag
      241 agtttcccta atccatatgg tgaacctaat actatctttg aaaatttatc aatcgatata
      301 gaagatggtg aatt
//
LOCUS       DACXMZ010000463          309 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.464, whole genome shotgun sequence.
ACCESSION   DACXMZ010000463 DACXMZ010000000
VERSION     DACXMZ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.464"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            21..309
                     /locus_tag="JD016_19890"
                     /pseudo
     CDS             21..309
                     /locus_tag="JD016_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          111 a           30 c           56 g          112 t
ORIGIN      
        1 attaataagg aggggtcaaa atgcctagca tgattatgga agttgtagga gtactattta
       61 tagctattgg attaatcttg acattcattg aaataaaagg aaatttccct tctttttatg
      121 gttttggtca aaattaaaat gctgtaggta ttatagcagc aggaattgct ttaattttct
      181 ttagaaatgg catagatagc atagattcta tagctactta tggtgtattt ttagcaatag
      241 aacttgttgt aatagttgca tatttcttaa taagaaaaaa atttactaaa gaagataaac
      301 atttaagaa
//
LOCUS       DACXMZ010000464          305 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.465, whole genome shotgun sequence.
ACCESSION   DACXMZ010000464 DACXMZ010000000
VERSION     DACXMZ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.465"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>305
                     /locus_tag="JD016_19895"
     CDS             <1..>305
                     /locus_tag="JD016_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAU5001837.1"
                     /translation="KYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVIT
                     DLQDNDYITWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLAT
                     "
BASE COUNT          116 a           39 c           67 g           83 t
ORIGIN      
        1 aaatatagtg gtatcagagg aaatgactta aaagtaacag ttacaacaaa tatagatgat
       61 aatgctaagt ttgatgttgt aacactttta gataataaga aggtagatac tcaaatagca
      121 aaggttatta cagacttaca agacaatgac tatatcactt ggaagaagga tgcaacacta
      181 gaagcaagtg caggacttgt atttactggt ggaactaatg gcgaagctgt gacaggagca
      241 gagtaccaag ctttcttgga taaaatagaa agctatagct ttaatgcttt aggatgtttg
      301 gctac
//
LOCUS       DACXMZ010000465          293 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.466, whole genome shotgun sequence.
ACCESSION   DACXMZ010000465 DACXMZ010000000
VERSION     DACXMZ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.466"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
BASE COUNT          127 a           28 c           32 g          106 t
ORIGIN      
        1 gtcattaaat atgccaatag ttttatatat tattgatttt attaaaaaaa gagatataca
       61 aactaataag ttgtaatctc tcaaatataa aaaaacaata aattagctca ttggattttt
      121 tagtccataa tataattata gattattcaa caaacaatct atagaaattg tggtattaat
      181 gtttaaataa ccaacttttt ctaaaataac ctttatagga atatatgagt tcataagtaa
      241 agatagcgaa ataagaaacc agtttggtat gtaaaatcta gatttttaat taa
//
LOCUS       DACXMZ010000466          291 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.467, whole genome shotgun sequence.
ACCESSION   DACXMZ010000466 DACXMZ010000000
VERSION     DACXMZ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.467"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            28..102
                     /locus_tag="JD016_19900"
     tRNA            28..102
                     /locus_tag="JD016_19900"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60..62,aa:Thr,seq:tgt)
     gene            117..201
                     /locus_tag="JD016_19905"
     tRNA            117..201
                     /locus_tag="JD016_19905"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:151..153,aa:Tyr,seq:gta)
     gene            211..284
                     /locus_tag="JD016_19910"
     tRNA            211..284
                     /locus_tag="JD016_19910"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:243..245,aa:Gly,seq:tcc)
BASE COUNT           74 a           76 c           71 g           70 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaaatgct ggtgtggctc aacggtagag cagctgactt
       61 gtaatcagca ggttgtaggt tcgattccta tcaccagctc catgtaaaac taatacggag
      121 gatttcccga gcggccaaag ggggcagact gtaaatctgt tgtcatcgac ttcggtggtt
      181 cgaatccacc atcctccacc aattaaatat gcgggtgtag ctcaatggta gagttctggc
      241 cttccaagcc agctgtgagg gttcgatccc cttcacccgc tccaaagaaa g
//
LOCUS       DACXMZ010000467          269 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.468, whole genome shotgun sequence.
ACCESSION   DACXMZ010000467 DACXMZ010000000
VERSION     DACXMZ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.468"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>269
                     /locus_tag="JD016_19915"
     CDS             <1..>269
                     /locus_tag="JD016_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAU5001838.1"
                     /translation="NKILPGAYINFISAKRATSSLSDRGIVAMPIELDWGINEEVFQV
                     TSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKA"
BASE COUNT           98 a           24 c           54 g           93 t
ORIGIN      
        1 aataaaatac ttccaggtgc ttatataaat tttatctcag ctaagagggc aactagttca
       61 ttatctgata gaggtattgt agcaatgcca attgagcttg attggggtat taatgaagaa
      121 gtatttcaag taaccagtga tgattttgag aagtattcag taaagtattt tggatatgat
      181 tatactcatg agaagctgaa aggtttgaga gatttattta aaaacataag attaggttat
      241 ttttataaat taaataaagg tgttaaagc
//
LOCUS       DACXMZ010000468          269 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.469, whole genome shotgun sequence.
ACCESSION   DACXMZ010000468 DACXMZ010000000
VERSION     DACXMZ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.469"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>269
                     /locus_tag="JD016_19920"
     CDS             <1..>269
                     /locus_tag="JD016_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAU5001839.1"
                     /translation="NKVLPGAYINFVSATRATSSLSDRGIVAIPLELDWGIDEDVFQV
                     TSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKA"
BASE COUNT           93 a           27 c           61 g           88 t
ORIGIN      
        1 aataaggtcc tacctggtgc atatataaat tttgtaagtg ctacaagggc aaccagttca
       61 ttatcggata gaggtattgt tgcaatacct ttagagttag attggggcat agatgaagac
      121 gtatttcaag taaccagtga tgattttgag aagtattcag tgaagtattt tggatatgat
      181 tatactcatg agaagctgaa aggtttgaga gatttattca aaaatataag gttgggatat
      241 ttttataaat taaataaagg cgttaaagc
//
LOCUS       DACXMZ010000469          266 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.470, whole genome shotgun sequence.
ACCESSION   DACXMZ010000469 DACXMZ010000000
VERSION     DACXMZ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.470"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>266
                     /locus_tag="JD016_19925"
     rRNA            <1..>266
                     /locus_tag="JD016_19925"
                     /product="23S ribosomal RNA"
BASE COUNT           89 a           47 c           75 g           55 t
ORIGIN      
        1 aagggtgcag ggcggatgcc ttggcactag gagccgatga aggacgtgat aagctgcgat
       61 aagcttcggg gagttgcacg taaactttga tccgaagatt tccgaatgag gaaactcact
      121 tagagtaatg tctaagtatc attaagtgaa tacatagctt aatgagggga actcagggaa
      181 ctgaaacatc taagtacctg aaggaagaga aagaaattcg attccgtaag tagcggcgag
      241 cgaacgcgga ttagcccaaa ccaata
//
LOCUS       DACXMZ010000470          265 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.471, whole genome shotgun sequence.
ACCESSION   DACXMZ010000470 DACXMZ010000000
VERSION     DACXMZ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.471"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
BASE COUNT          110 a           22 c           35 g           98 t
ORIGIN      
        1 agattagaag tgtttgataa ttacagtaca tgttagatat atcattgtaa attaacataa
       61 ctttgaatat aataatgttc agaaggcaat atttttgagg tatgaatcca ataatatagt
      121 cttatgattg tagatatcaa aaaatataag ttactaggaa tacgttttgt agatttatat
      181 atgattaata actaaagcta taaataaaca aaataaaaag agtatgtttc aacctatagc
      241 ttttagattt aaaatatact ctttt
//
LOCUS       DACXMZ010000471          243 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.472, whole genome shotgun sequence.
ACCESSION   DACXMZ010000471 DACXMZ010000000
VERSION     DACXMZ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.472"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
BASE COUNT          107 a           10 c           49 g           77 t
ORIGIN      
        1 tataattata tagaatgttt caattaaaca aggtaaaaac tataatctta aaattaaaat
       61 taacattatt cttaagaagc aagaaattta gaaagtagga agtttagtga taagtggtag
      121 taagtaagaa atttagtaag tgggaagttt agtagtaata atttatagca agtaagaaat
      181 ttaggaagta ggaagtttag taataatttg tagcaagtaa gaaacttagg gagtaggaag
      241 ttt
//
LOCUS       DACXMZ010000472          232 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.473, whole genome shotgun sequence.
ACCESSION   DACXMZ010000472 DACXMZ010000000
VERSION     DACXMZ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.473"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>232)
                     /locus_tag="JD016_19930"
     CDS             complement(<1..>232)
                     /locus_tag="JD016_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20 family protein"
                     /protein_id="HAU5001840.1"
                     /translation="NVIQKIQQLVPPTHPVLGDGILVLTDIKSSPYPGASVVPDYCKA
                     TFDRRLLVGETREGVLAPIQALLDEMMKEDPE"
BASE COUNT           66 a           41 c           37 g           88 t
ORIGIN      
        1 aattctgggt cttctttcat catttcatct aataaagctt gtattggagc taatacgcct
       61 tctcttgttt ctccaactaa taatcttcta tcaaaagtag ctttgcaata atctggcact
      121 actgatgctc ctggatatgg agaagatttt atatctgtta aaactaatat tccatcacct
      181 aaaactggat gagttggagg tactaattgt tgtatctttt ggattacatt ag
//
LOCUS       DACXMZ010000473          220 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.474, whole genome shotgun sequence.
ACCESSION   DACXMZ010000473 DACXMZ010000000
VERSION     DACXMZ010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.474"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>220
                     /locus_tag="JD016_19935"
     CDS             <1..>220
                     /locus_tag="JD016_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001087426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HAU5001841.1"
                     /translation="KLMEKMTEARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDGG
                     TSLNRQTTDIDTVKIIKVPSNLMKTAYD"
BASE COUNT          103 a           28 c           34 g           55 t
ORIGIN      
        1 ataagttaat ggaaaaaatg acagaagcta gagtacctga aaatggaaga atattgtatg
       61 ttactccagt agtaaataca cttatcaaaa atgcaaaaga gatacaaaga acagtaaaca
      121 taaaagatgg tggaacttct ttaaatagac aaaccacaga tattgacaca gttaaaataa
      181 ttaaagtacc atctaatcta atgaaaactg catatgattt
//
LOCUS       DACXMZ010000474          216 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.475, whole genome shotgun sequence.
ACCESSION   DACXMZ010000474 DACXMZ010000000
VERSION     DACXMZ010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.475"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>216)
                     /locus_tag="JD016_19940"
     rRNA            complement(<1..>216)
                     /locus_tag="JD016_19940"
                     /product="23S ribosomal RNA"
BASE COUNT           42 a           61 c           47 g           66 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgt
//
LOCUS       DACXMZ010000475          215 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.476, whole genome shotgun sequence.
ACCESSION   DACXMZ010000475 DACXMZ010000000
VERSION     DACXMZ010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.476"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>215
                     /locus_tag="JD016_19945"
     CDS             <1..>215
                     /locus_tag="JD016_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001090112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001842.1"
                     /translation="KNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGD
                     STTMYPSQNISDEIKEKIVEYTKKIAL"
BASE COUNT           97 a           22 c           40 g           56 t
ORIGIN      
        1 aaaaatccag tacttataga taaatatcta actggtagag aaatagaagt agatgctata
       61 tgtgataagg aagatatatt gatacctggt ataatggaac atttagaaag agctggagtt
      121 cactctggag atagtacaac aatgtatcca agtcaaaata tatctgatga aataaaagaa
      181 aagatagtag aatacactaa aaaaatagct ttaga
//
LOCUS       DACXMZ010000476          214 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.denovo.477, whole genome shotgun sequence.
ACCESSION   DACXMZ010000476 DACXMZ010000000
VERSION     DACXMZ010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.denovo.477"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
BASE COUNT           64 a           33 c           17 g          100 t
ORIGIN      
        1 tttagctgtt tacttgctta tttaatagtt ttatttcttt gattaaatat ctttcagaat
       61 ctaagccaga tgtctcaaaa aaactgattt ataatataat cattttttct gctattctta
      121 tttttccagt tttagtttca tattaaaata tacttttcat agacactgca ttctttcata
      181 ctcattttca catttaacta tacaagtagt ctta
//
LOCUS       DACXMZ010000477        14231 bp    DNA     linear   BCT 15-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile strain CD07839
            SAMN09400641-rid8188303.guided.086, whole genome shotgun sequence.
ACCESSION   DACXMZ010000477 DACXMZ010000000
VERSION     DACXMZ010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09400641
            Sequence Read Archive: SRR7296935
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-SEP-2019
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000581637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000581637.1
            Genome Coverage       :: 136x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/08/2019 21:29:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 4.9
            Genes (total)                     :: 4,000
            CDSs (total)                      :: 3,921
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 28 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 28 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09400641-rid8188303.guided.086"
                     /strain="CD07839"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2017"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            206..823
                     /locus_tag="JD016_19950"
     CDS             206..823
                     /locus_tag="JD016_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAU5001843.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIIKPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            931..3462
                     /locus_tag="JD016_19955"
     CDS             931..3462
                     /locus_tag="JD016_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAU5001844.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKKALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNIKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3896..4597
                     /locus_tag="JD016_19960"
     CDS             3896..4597
                     /locus_tag="JD016_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanS"
                     /protein_id="HAU5001845.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4587..5729
                     /locus_tag="JD016_19965"
     CDS             4587..5729
                     /locus_tag="JD016_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAU5001846.1"
                     /translation="MKSKNIKVNPDFTQVRNKIFMRTALMAFIAIIFIWILYSFILYG
                     KFSIWIVSFFENAFRMEYKAALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTK
                     YFSEINQGIDSLIKEDVGEVALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELI
                     VYLAHDLKTPLTSVIGYLTLLRDESQISEELRKKYLSISLDKAERLEDLINEFFEITR
                     FNLSNISLEYSTVSLTRMLEQLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVF
                     DNLLRNAVYYSFEDTTIEITAVQEDDYIKLKFINYSNTIPEEKLERIFEQFYRLDTSR
                     NSNSGRAGLGLAIAKEIVELHNGTITAHSENNIITFKVIIPIVGNL"
     gene            5990..7090
                     /gene="vanG"
                     /locus_tag="JD016_19970"
     CDS             5990..7090
                     /gene="vanG"
                     /locus_tag="JD016_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HAU5001847.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7087..7893
                     /locus_tag="JD016_19975"
     CDS             7087..7893
                     /locus_tag="JD016_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAU5001848.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7911..10049
                     /gene="vanT"
                     /locus_tag="JD016_19980"
     CDS             7911..10049
                     /gene="vanT"
                     /locus_tag="JD016_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HAU5001849.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            10363..10983
                     /locus_tag="JD016_19985"
     CDS             10363..10983
                     /locus_tag="JD016_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAU5001850.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(11149..11685)
                     /locus_tag="JD016_19990"
     CDS             complement(11149..11685)
                     /locus_tag="JD016_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAU5001851.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(11798..12502)
                     /locus_tag="JD016_19995"
     CDS             complement(11798..12502)
                     /locus_tag="JD016_19995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAU5001852.1"
                     /translation="MKKKILIPVIMSLFIISQCITSFAFTPENNKFKVKPLPYAYDAL
                     EPYIDKETMKLHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVR
                     NNAGGAYNHKFFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWA
                     WLVATKDGKLSIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVV
                     NWNGALENYKNLKSQD"
     gene            12675..>14231
                     /gene="asnB"
                     /locus_tag="JD016_20000"
     CDS             12675..>14231
                     /gene="asnB"
                     /locus_tag="JD016_20000"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAU5001853.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRME"
BASE COUNT         5468 a         1506 c         2441 g         4816 t
ORIGIN      
        1 gaaataaaat atatttttat tatttcaaca atgaaaaaga ggcttgtttc cattttgaga
       61 cagtataatt tttatgtaac tttaatttta gtaaatataa agttttaaaa ttgataaaat
      121 aggaggaatt tttaatataa aagggtaaat aaagggttta gagggcataa tatagaataa
      181 attaatatat ttaaggaggg tttttatgtg taaaaaaacg tatttattag tggtaagtat
      241 gcttatagcg ggtttattaa ctgcatgtag tgggagtcca actaaggatt caggacaagc
      301 acaagataat acaaaaaagg aggcaaatgc atcaaataac gctttaaaag atgaaaaaaa
      361 taatgaaaat ttaatggaac aagattttaa agtgccatac acagatgcaa taaacatatt
      421 taaagataaa tataaggatg ctgatatagt agatttaagt cttgagagag atttaaataa
      481 atttgtttat actgtggaag gtgtagatga taataatgaa tataagatga aaattgatgc
      541 taatacaaaa gatgtattag aagataaaac agaaaagttg gattcagaag atttaaatgg
      601 agttgctaga aaagaaaaat tagatttaaa tgacataatt aaacctcaac aagctatgga
      661 aattgcatta aaagaacaaa atggaatggt taaagaatgg agtttagaca aagatttaga
      721 tgttactttt tataaaataa gaatagataa agataaaaat gaatatgaca taaaagtaga
      781 ttctaaaaag ggaactgtat tgaaggtaga aaaagaagat tagaagattt aaactatctt
      841 taaaataatt tcttttttga ataaattttg tttaaaatat aacaagcagg gtataaagag
      901 actaaataac tattttagga ggagtacggt atgtcattga aatcactaaa aataaaagaa
      961 aatttatatt gggtaggttc acttgaccct gatttaagag tatttgacat aattatgtat
     1021 acaccttatg ggactactta taattcatat gtattaaaag gaacagaaaa aacagtttta
     1081 tttgagacag ttaaagataa gcattttgac aattatattg aaagattaaa tgatttaaat
     1141 atagattttg aaaaaataga ctatattgtt gtaagtcata ctgaaccaga ccatgcagga
     1201 agcgttgaaa agcttttgga tttagctaaa aatgcaaaag tagtagcttc agaaacagca
     1261 attaaatatc ttaaggaaat cgtaaacaaa gactttgaat atgttgcagt tacagatgga
     1321 gatacattat caattgggga caaaacttta gaatttttct ctgtacctat gcttcattgg
     1381 ccagatacca tatacacata tataaaagag gataaaactc ttgtaacatg tgattcattt
     1441 ggtagtcact atagtaatga taagatagta aatacacttg atgaaaatga agaaaaggat
     1501 tacttggatg ctttaagata ctattatgat tgtataatgg gtccatttaa accatctatg
     1561 gtaactgcta tagaaaagat aaaagattta gatatagata ctgtatgtcc aggtcatggt
     1621 ccagtattaa ctgaaaatcc tagaaaaatt atagaccttt attacaactg gagtgtaaat
     1681 gaacaaataa agttagaaaa agaagttaca atttgttatg tttctgcaca tggatataca
     1741 aagattatgg cagaagctat aaaagcatat attgaaaaaa atagcaacta taaagtaaat
     1801 ttatttgatg taatagagca taagcaagaa gatattttgg caaaaatagc agtatcacaa
     1861 ggtgtattat ttggaacacc aacaatatta ggagatgcgt taaagcctat atgggatata
     1921 ttaatatcgt taaacccagt tcttcatggt ggaaaagttg cttcagtatt tggttcatat
     1981 ggatggagtg gagaaggtat agaaaatgct atggagagaa taagtcagct tagaatgaca
     2041 gcagtaaaac cttttgctgt taactttaaa ccatcaaatg aagagattga taaattacat
     2101 tcttatacag gtaaattcct agataagtta aattctactt ttggaagtaa aaagaaaact
     2161 aagaaattta aatgtgtaat ttgtaatgaa gtttttgaag gtgatagtgc tcctagtgta
     2221 tgccctgtat gtggagctaa ggaagaccaa tttatagaag ttgaagaaga tgaagttact
     2281 tttagaaaag atactgatga atatttcgta atagtaggaa atggagcagc tggtttttat
     2341 gcagcagatg ctataagaaa aagaaataaa acttgtaaga taactatgat ttctaatgaa
     2401 gatgagttga cttactatag acctgcactt tctgatggta taaatgaaga acttggttca
     2461 gatttctaca tggaagataa agattggtat gataaaaata atatagtagt aatattagga
     2521 acgaatgtag ataaattaga tgaagtgaat aaaactatta tagtgaatga tggagctatt
     2581 aaatttgata agcttgtaat agctacagga agtagaaact ttataccacc aattaagggc
     2641 catgatttag aaaatgtgtt tactcttaga aatataaaag atttatatag tgtaaaaaaa
     2701 gcactagaaa aatctaagaa agttgtagtg attggtggcg gtttattggg acttgaagct
     2761 gcatgggaat ttagactaaa aggattagaa gtagtagtag ttgaagccat ggatagcata
     2821 ctatcaaaac aacttgataa agaaggtagt aaaatattag aacaatgtgt aagagataca
     2881 ggaatagatg taagattggg tgtagcagtt gatggtattg aaggtgatgg aaaagctcaa
     2941 aaagttgtat ttaaagatgg agatagtgtc gactgtgata tggtagtatt ttcaattggt
     3001 gtaagagcaa acactcaaat ggttcaggat acatctgtaa aaattgacag aggtattgtt
     3061 gtagataaga cattacagac aaatattaaa gatatatatg cttgtggaga tgttgcacaa
     3121 gtgggaaata ttagtttagc aatatggcca tcttcagtag aaatgggaaa aatagcaggg
     3181 gcaaatgcaa gtggagataa tttgacattt gaaagtgaag tatatccagt ttcattagat
     3241 gcaatgaatg taaaagtatt tagtattggt aatatacaaa attttgataa agaaatttca
     3301 tcaaaagatg aaggtcaaag aatatataaa aaattattta tgaaagatgg ttcattagtt
     3361 ggagctatac ttataaatga tttaagctgt actgttaaat tgataagatt aatatctgaa
     3421 aaaggtgatt ttgaggatat tatgaaggct gatatactat aatttaaata cattaaaata
     3481 aaaaggagtt gtcttaaaat tgattttttt ctaatcaaat tttaaattca actccttttt
     3541 tgaattttta tataaaataa catggaaatt ttagtaatgt ctgtggttac atacataggt
     3601 ttaattgttt tttatatgga ttgtctctgg tataataaat tgaattgtgt ttgactgata
     3661 gatattttta ttgaaagtta atacctgtta tgcgataata aggacgtgtt aggggagttt
     3721 ttataataac aattaatctc agtttttgtg ttccaatggt agcatcaaca cattctgatg
     3781 tagtagttct tttcttataa aaattagtta caaatatgct tgtaattatt tgatatgtaa
     3841 atttaccaat attataaatg atgacctcga taataaggag agtgaaaaat aatctatgaa
     3901 cgaaaatatt ttgattgtgg atgatgaaaa agaaatagca gatttaattg aagtatattt
     3961 aaaaaatgaa ggttttacag tatacaagtt ttatacaggt aaggaagctc tagaatgtat
     4021 tgaatctaaa aatttggata tggctatttt agatattatg cttcctgata ttgatggatt
     4081 tcatatttgc cagaaaattc gagaaaaata ctattatcct atcattatgt taacagctaa
     4141 aattgaagat atagacaaga taatgggttt aaccataggt gctgatgatt atataacaaa
     4201 gccatttaac cctttggaag ttgttgcaag agtaaagaca caattaagaa gatatatgcg
     4261 atataataat tcttatgaac aacagtcgat aattgttaat gagtatgata ttaaaggatt
     4321 gattatcaat aaagaaacac ataaatgtag tttatttggg aaagaagtag cattaacacc
     4381 gattgaattt tctattcttt ggtatttatg tgaacatcaa ggaaaagtgg ttccttctga
     4441 agaattgttt gaagctgtat ggggagaaaa atatcttgat aataataaca cagttatggc
     4501 tcatataggg cgattacgtg aaaaaattca tgaaccttct aaaaacccga aatttataaa
     4561 aactgtatgg ggagtaggtt atacaattga aaagtaaaaa tattaaagtc aatcctgact
     4621 ttactcaagt gagaaataaa atttttatga gaacagcttt aatggcattt atagccatta
     4681 ttttcatatg gattttatat tcttttatat tgtatggtaa gttttctatc tggatagtat
     4741 cattctttga aaatgctttt cgtatggaat ataaagctgc attgaattta tatcagcaga
     4801 ttttccgtaa tagcatggag ctcttcatga tactagctat ggtgattgtg ttttttataa
     4861 tattccgtgt ttatttaaat tggtttacaa aatattttag tgaaataaat caaggtattg
     4921 actctcttat caaagaagat gttggagaag tggctttatc tccagagtta ttggcaatag
     4981 aaaagaaaat aaactcaatt aaacatatac ttgaacaacg taaatttgag acacaaatgg
     5041 cagaacaacg taagaatgaa cttattgttt atcttgcaca tgatttaaaa acaccactta
     5101 catcagttat agggtatctg acactacttc gagatgaaag tcagatttct gaagaactgc
     5161 gaaaaaaata tttatctatt tcgttggata aggcagagcg tcttgaagat ttaattaatg
     5221 aattctttga aattacaagg tttaatttat caaatatatc acttgaatac agtacagtaa
     5281 gcttaactcg tatgcttgaa caactaacat atgagtttaa acctactttg atgagtaaga
     5341 atttaaaata tacactgaat gttacaccaa atatgatgat aaggtgtgat gttaataaaa
     5401 tgcaacgtgt atttgataac ttattacgaa atgctgttta ttatagtttt gaagatacta
     5461 ctattgaaat aacagctgta caggaggatg attatataaa gcttaaattt ataaattata
     5521 gcaatacgat tccagaggaa aaacttgagc gtatttttga acaattttac aggttggata
     5581 catctcgtaa ttcaaacagt ggtagagctg ggttgggact tgctatagct aaagaaatag
     5641 tggagcttca taacgggaca ataacagctc atagcgagaa caatataatt acctttaagg
     5701 ttattattcc gattgtagga aatttgtaag aatttaagta taaaaaaata ggaaattctt
     5761 taggataaat aaataaagag cactcttgtt attgatataa ttactgtatc aaaacaagag
     5821 tgcttttatt ttgatacagt aattatatca atagaaaaat gtgaaaatat attatgtgga
     5881 acagatgcaa ttgtaagtga aggttgattg gatttgaaat agaaaaagta ttttaacatg
     5941 tttctgtaga ttgtataaaa ataaataaat caacaaagga gaatcaaaga tgaataataa
     6001 aaaaattgca gttatatttg gtggaaactc aacagaatat gatgtatcat tacaatcagc
     6061 ttttgcagta cttgagaatt tgaatacgga aaaatatgat attataccaa ttggaattac
     6121 aagggagggt aattggtatc attatattgg agattataat aatatccaga ataacacttg
     6181 gtttgaaagg gatactgata taattccagt tattgtttcg cagaaccgtg tcaaaaatgg
     6241 tattattgaa tttttaattc ctgatgctgt ttctcagaat catattccaa atagtgctat
     6301 tgaatcatgt gaaaatgata ttaaatacac taaacttgat tttgtatttc ctgtattgca
     6361 tggaaaaaat ggagaagatg gaacagtgca ggggttattt gaattggcag gtatacaggt
     6421 tattggatgt ggtactcttt cttcagcatt atgtatggac aaagacaaat cacataaact
     6481 tgttcattca gaaggtatta aagtaccaaa atcaatatta ctaaaatact cagataaatt
     6541 taatgtggct gaactgtcaa aagacttaac ttatccatta tttataaaac ctgtgcgtgc
     6601 aggttcatca tttggtatta gtaaaattta taaaaaagaa gagttacaag tagcagtaga
     6661 cttggctttt gaacatgata atgaagtaat aattgaggaa aatattgatg gttttgaggt
     6721 agggtgtgca gttttaggaa acaatgactt acttgttgga agagtagatg aaatagaatt
     6781 gtcaaaagga ttttttgatt atacagaaaa atatacattg aaaagttcaa aaattcatat
     6841 gccagctaga attgatttgg attgtgagaa aagaatacaa gagacagcaa aaattattta
     6901 tagaactctt gggtgttctg gatttgctag agtagatatg tttttaactc catctggtga
     6961 aattattttt aatgaagtca atacaattcc tggctttact tctcatagtc gttatccaaa
     7021 tatgatgaaa ggtattggat tgtcttttga agaaatattg gacaagataa ttgaggtgta
     7081 cataaaatga tgaataatat tttattagat aaaaataaag aagataaaat gacaaatact
     7141 atcttattga ctaaagataa agtttatgat gggaatctaa ttctagtaaa tgctttttta
     7201 ccagttaaaa catctgaaga tatagattta attcctgtag atacaaggtt tccaagtatt
     7261 cttatgaaac gtgaagctac aaacatttta caaaatattt taaaaagtat atgtggaata
     7321 aatgaaatag tacctgtaag tggatatcgt actgcagagg aacaacaaga tatatattca
     7381 agttctctgc gtgacaatgg aaaagacttt actaaaaagt ttgtagcact tccaaatcac
     7441 agtgaacatc agactgggct tgctattgat ttagggttaa agaaagaagt tatagatttt
     7501 atctgccctg aatttcctta taatggtatt tgtaatgagt ttcgtaaggc agcactacat
     7561 tatggtttta tagagcgtta tcagtgtaaa aaagaagaaa taacaggaat atctcaagaa
     7621 ccatggcatt ttcgttatgt gggatatccg cattctgaga ttatcaataa aatgaatctc
     7681 gctcttgaag aatatataga atatattaag agattttcgt atacaaattg tttaaaaata
     7741 gaagataaag aaaaaattat agaaatcttt tatgtgccta ttcatactaa agaagaagtg
     7801 agaatctcta tacctgaaca atgtgtatat caaatatcgg gtaacaataa ggatggtttt
     7861 attgtgacat tttggagaaa tagaaaggac taagaaatgg aggaacaaga atgattagga
     7921 ataaatctta tgcagggctt gattatttta ggattatagc tgcattactt ataattgcaa
     7981 ttcatacatc tccattatta tcatatagta aaatggctga ctttgtgtta actagtataa
     8041 ttgctcgtat tgctgtgcca ttctttttta tgctatcagg cttttttcta atttcaacat
     8101 ataattataa ttgtaataaa ctgaaatctt ttctaaaaaa tgtattattt atttacggga
     8161 tagctattgt aatttatatt cccttaaata tttacactgg atatttttcc atgaataatt
     8221 tagtgcccaa tattattaag gacattgtat ttgatgggac tttatatcat ttatggtatc
     8281 tacctgcatc aattattggt gcaagtattg catactttct tgtaaaaaaa ctaggtttaa
     8341 agtactcatt agtaatcact attgttattt atgtaattgg tatgtttggt gatagttatt
     8401 atggtttttt agaaaaaata ccactattaa agaatttata tgggtatatt tttgaagtat
     8461 cagattatac acgaaatggt atattttttg cacctgtatt tttcatactt ggtggaataa
     8521 tatctaataa atctaattat atgtcaacta aaaatagctt gattggattt cttatatcat
     8581 tcttacttat gttatgtgag ggaatgtttt tacataaact aggggtacag cgtcatgata
     8641 gcatgtatat tatgttattg ccatgtatgt atttcttatt tacagcctta acatttttga
     8701 aaggttctcg agctaggtta ttgcgaacat catcattaat tatttatatt atacatccta
     8761 tgatgattgt tgttattcgt atgttttcta aaatattcgg aatacaaact gtcttggttg
     8821 acaatagcct tattcattat atagtggtaa gtatagcatc ttttatagct tctataatta
     8881 ttatattgat tcttaaacct ctaaaaaaag acttgaaagg agaacataag gtaaatacca
     8941 atagagcttg gattgaaatt gatttaaata atttggaaca taatgtaaag atgatacaaa
     9001 aagctatgcc agaagattgt gaattgatgg cagttgtaaa agctaatgcc tatggtcata
     9061 gtgcttttga agtagcgact tgtgcaaacc aaataggtgt aaaagcattt gcagtagcaa
     9121 caatagatga ggggattgat ttacgtcgat atggaatact aggagaaata ttgattcttg
     9181 gatatactaa cccaataagg gcaaaggaac ttcataaata caatcttaca cagagtgtta
     9241 ttgaccttaa tcatgctatc tctttaaaca aacagaaata caacataaaa tcacacatta
     9301 aaattgatac aggtatgcac agactgggtt ttgatgttaa tgatgtgagg tcaattttaa
     9361 aggcatttaa attaaaatac ttagatatat gtggcatatt tacacacttg tgtgtttcag
     9421 atagtctgaa agatgaagat gtagacttta caaataaaca aattgaatgt ttttataagt
     9481 taattgattt tctgttaaaa aaaggtataa aaattcctaa agtacatatt caaagtagtt
     9541 atggtttttt taattatccc aatcttaagt gtaattatgt aagaattgga attgcattat
     9601 acgggatatt aagttctcca aatgatacga ctaaattgca acttgattta cgacctgttt
     9661 tatccttaaa atcacaagta attcttatta gaaaaataca aaaagatgat agttttggat
     9721 atggaagagt ctttattgca agcagagata gcgtaattgc tatattatct attggctatg
     9781 ccgatggtat acctagaaat ttatcctgtg gaaaaagtta cgtaataata aatggttgta
     9841 gagcagcaat agtgggtcga atttgcatgg accaacttgc tgttgatatt acagatatac
     9901 ctaatgtaga agttggtaat acggcaataa taataggaag agataattta tctgaattat
     9961 ctgcttctga agttgcaaat aactcatgta gtatatcaaa tgaattattg agcagagtag
    10021 gaaggaggtt aaatgttata aagaagtaac tcaattaaat agtaatattt gttgtactgt
    10081 gtaatttatt ttactctgac tagacatttt tagtaatatt atgtctaata tatatttttt
    10141 ataacttaca tgacaattta agctataaaa aggtgatgtt tcagattttt attttgaaac
    10201 atcacctttt ttgttataat atataataaa ataattatac cttaactgta aacttatata
    10261 gattatttac tttttaatat tagattcatt ttttgagatt ggtatataat aaacatatag
    10321 agtatataat aatagttaaa gttaaaaaag gggaaaagca atatgcagta tatacaagaa
    10381 ctacttgatt tgactcaaaa ttattgggtg ttagccacaa taatcggctt attaagtgca
    10441 tttatagaaa gctttatacc agccttacca cttgttgcta tagttacagc aaatgctgca
    10501 atacaaggat tatttattgg atgtttgtta tcatggattg gctcaggact tgggacaact
    10561 tcattattct tattaataag cagatttaat gatagtaagc tttttaataa gttgagaaat
    10621 agtaaaactg aaaaagcaat aagttggatg gataaacagg gatttaagtt attatttata
    10681 gcgtatgcct gtccatttat gcccggttgt ttagttacta tcgcatcagc ttttggtaaa
    10741 aaagatttaa aagattttgt gccagcaatg ttggcaggaa aatttgtaat gtttatagtt
    10801 ataagttacg tggcaagtga tatagaaggt tttataacaa gtcctttaaa gatagcatca
    10861 tttatattat tggtgttttt gtcatggaag attggaaata gagtaaataa aaatcttgaa
    10921 aatcataatt atgatttcca tcataaaaaa catcacaatg ataaagatga taaaatgatt
    10981 tagcttgtac gaaataaaac gtatcttaaa atgatttagt actaaattat tttaagatac
    11041 gttttatttt ttgtattaaa gtacaaaatt tttagttctt ttgtaaacta aaatatataa
    11101 aacttattta tacttagttc aaaatattgt tatttatttt ttctacaact ataataaaat
    11161 ataaaattga tatccaatca aaaaaataac tatgaaattt aatagcttag atatgaattt
    11221 ttctgttttt gagttagttt ttataggtag taaagaaatg tttatggtaa tgggataaaa
    11281 caattcaata ccttctttag taataaggtc aagacacaag tgagatagat atcctattat
    11341 aaacccactg gacaaacatg agaaaactat attattgtta gtgaagattg tcagagattt
    11401 aaaaaaacta taaataagta ataaggcaat tagactgtga gtaaaccctc tatgtctaaa
    11461 cctacttcca aataatttgt atattaacat aaatttttta cttatgtaag aacctctcat
    11521 atctatatct ggaaataaag aacctaaata tgcaaaataa atataaatta taattaatac
    11581 tattttatat gcaatatcgt atttaactag gtatagattg tttataaagg ggagtatcaa
    11641 aagtgcgaat atataacctc ccctttcatg tgtttctttt atcatataaa aatcctcctg
    11701 aatcgtcact ataaacatta tattggtaaa tttagactat cttttattat attatatagc
    11761 aaacataagt ttttttcaaa aggattttaa tatttaatta atcttgagat tttaaatttt
    11821 tgtaattttc taaagcacca ttccaattta ctacattaaa ccagttgtca atgtattcat
    11881 ttcttctatt ttgatatttt aaatagtaag catgctccca aacatcaagt cctattatag
    11941 gagttaggtt tttacttaca gggctatcct gatttggagt agtcataata gataatttcc
    12001 catctttagt agctacaagc caagcccaac cagaaccaaa gacatctaaa gcagattttt
    12061 ggaactcttg cttaaatttt tcaaaagaac caaagtctct atcaatagct tcttttaaag
    12121 attcagaagg tatggttttt tctggtgtca ttatatcaaa aaagaattta tgattataag
    12181 ctccacctgc attatttctt acagttgtag caatatcttt aggtaaagaa tctaaatttt
    12241 gcaataattc acataaagaa taattataaa gctcaggata tttttcaaga gcagcattta
    12301 atttatcaac ataagcttga taatgcttat catgatgcag tttcattgtt tctttatcta
    12361 tataaggttc aagtgcatca tatgcataag gtaatggttt aaccttaaat ttgttatttt
    12421 caggtgtaaa agcaaatgaa gttatgcact gtgagattat aaataaagac ataataactg
    12481 gtattaatat ttttttcttc ataaaaatca cctcctaata tagtatttct agaaataaaa
    12541 aaaatatgaa atattaacag ataatttata agaatttaat agcaatttca ttttaaattg
    12601 aataagtttt aaagccaaag acaaaactat gaaatataga ataagactaa aggacagaaa
    12661 ggaattttga ttatatggac ggcaatattt caatttatta taaaggtgat atatctaaaa
    12721 aagaagatat taaatttaaa ataaaagaat ctgtaaataa tacaagactt tctagtagaa
    12781 tagagtataa ggatgaggaa aaaacttttg attttgaaag atttaatgta attgatgcta
    12841 tttatggaaa taaaccattt gaaaaagatg gaaaagttat tgtttttaat ggaaatatat
    12901 ataactatca tgaaataaaa gaagaattaa ttaaaaaagg acacaatttt actacaaatg
    12961 atgatattga agtcttatta gcatcttata tagaatttgg taagaattgt gtacataaga
    13021 ttaaaggaat gttcaacttt ataatctatg atagagaaaa tgaaagtata tttggagcaa
    13081 gagatttatt tgggataaaa ccactttatt atataaataa ggaaaatgca ataatttttt
    13141 cctcagagta taagttttta cttgaatata tgaaaaattt aaatataaat gaaagaagct
    13201 tacaaagtta tttttcattt caatatgtac ttcctgaaga tactatgata caaggaataa
    13261 ggctgatacc agcaggtcac tattttaggg tagaaaatgg tatactatct ttaaagagat
    13321 ataataaact tgagtttaga agtagtacaa aattttttta tactaaaaat cacttaggca
    13381 atagagatgt gaacgaggat gatataagaa atatagtagt agattcaata gtaactcata
    13441 tggaagagga gaaagaaatt ggaacatttc tgtctggtgg aatagattca tctattataa
    13501 ctactgtggc ttcacaaatt aatccaaata taaaatcttt ttctgcaggc tttagtgtca
    13561 aaggatatag tgaattagaa gtagcaaaga aaacagctga taagttaggt atagaaaata
    13621 tccagattaa tataacacaa gatgaatata taaaatcatt accgaatgta atttattctt
    13681 tggatgaccc tatagcagac ccttcagaag taggtcttta tttcttaatt aaagaggcag
    13741 gtaaacatgt taaagtagca ttgtctggag aaggagcaga tgaattattt ggaggatata
    13801 atatatataa agagtatagt actatgaaat cagttgtcaa ttcacctact tatattaaaa
    13861 gtatattagg aagagtttca gaacttatgc caaacataaa aggaagaaat tatctttata
    13921 gagctacaac accattagaa aaaagatata taggaaatgc aaaagttttt gaaaacagcg
    13981 aagtaaaaag attctttttt aagtataagg aaaagaatat atatgaatac ctgttgtcta
    14041 atttgtatag agatgcacaa aaaaataatt acgattatat aagtaaaatg caacatatag
    14101 atgtgaatac atggcttcaa ggcgatatac ttcaaaaaat tagcaaactt tctacagctg
    14161 agcaggttga acttagagta ccatttttat ataaagatgt atttgatgta gcaaaaaatt
    14221 taagaatgga g
//
